LOCUS       DAZFKF010000001       135986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFKF010000001 DAZFKF010000000
VERSION     DAZFKF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 135986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 135986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..135986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.001"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(231..1823)
                     /gene="lsrK"
                     /locus_tag="ACHKA1_000001"
     CDS             complement(231..1823)
                     /gene="lsrK"
                     /locus_tag="ACHKA1_000001"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO5677721.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1902..2855)
                     /gene="lsrR"
                     /locus_tag="ACHKA1_000002"
     CDS             complement(1902..2855)
                     /gene="lsrR"
                     /locus_tag="ACHKA1_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO5677722.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3104..4639
                     /gene="lsrA"
                     /locus_tag="ACHKA1_000003"
     CDS             3104..4639
                     /gene="lsrA"
                     /locus_tag="ACHKA1_000003"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO5677723.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYVRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAVGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRFSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4633..5661
                     /gene="lsrC"
                     /locus_tag="ACHKA1_000004"
     CDS             4633..5661
                     /gene="lsrC"
                     /locus_tag="ACHKA1_000004"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO5677724.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKTAS
                     SGKKREAA"
     gene            5661..6653
                     /gene="lsrD"
                     /locus_tag="ACHKA1_000005"
     CDS             5661..6653
                     /gene="lsrD"
                     /locus_tag="ACHKA1_000005"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO5677725.1"
                     /translation="MRIRYGWELALAALLVIEIVSFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLSLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6665..7687
                     /gene="lsrB"
                     /locus_tag="ACHKA1_000006"
     CDS             6665..7687
                     /gene="lsrB"
                     /locus_tag="ACHKA1_000006"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO5677726.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7714..8589
                     /gene="lsrF"
                     /locus_tag="ACHKA1_000007"
     CDS             7714..8589
                     /gene="lsrF"
                     /locus_tag="ACHKA1_000007"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO5677727.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8613..8903
                     /gene="lsrG"
                     /locus_tag="ACHKA1_000008"
     CDS             8613..8903
                     /gene="lsrG"
                     /locus_tag="ACHKA1_000008"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO5677728.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8960..9718
                     /gene="tam"
                     /locus_tag="ACHKA1_000009"
     CDS             8960..9718
                     /gene="tam"
                     /locus_tag="ACHKA1_000009"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO5677729.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9722..10636)
                     /gene="yneE"
                     /locus_tag="ACHKA1_000010"
     CDS             complement(9722..10636)
                     /gene="yneE"
                     /locus_tag="ACHKA1_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO5677730.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10833..12284)
                     /gene="uxaB"
                     /locus_tag="ACHKA1_000011"
     CDS             complement(10833..12284)
                     /gene="uxaB"
                     /locus_tag="ACHKA1_000011"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO5677731.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12511..13929)
                     /locus_tag="ACHKA1_000012"
     CDS             complement(12511..13929)
                     /locus_tag="ACHKA1_000012"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5677732.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14068..14427)
                     /gene="yneG"
                     /locus_tag="ACHKA1_000013"
     CDS             complement(14068..14427)
                     /gene="yneG"
                     /locus_tag="ACHKA1_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO5677733.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14427..15353)
                     /gene="glsB"
                     /locus_tag="ACHKA1_000014"
     CDS             complement(14427..15353)
                     /gene="glsB"
                     /locus_tag="ACHKA1_000014"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO5677734.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15417..16805)
                     /gene="sad"
                     /locus_tag="ACHKA1_000015"
     CDS             complement(15417..16805)
                     /gene="sad"
                     /locus_tag="ACHKA1_000015"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO5677735.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVTQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIAAAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16906..17787
                     /gene="yneJ"
                     /locus_tag="ACHKA1_000016"
     CDS             16906..17787
                     /gene="yneJ"
                     /locus_tag="ACHKA1_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5677736.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            17865..18980
                     /gene="yneK"
                     /locus_tag="ACHKA1_000017"
     CDS             17865..18980
                     /gene="yneK"
                     /locus_tag="ACHKA1_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO5677737.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICNE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPARNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMINPGVPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQE
                     SPHFQDLFLFFASMLLHYHQFMNPNESDLNNVLMPASLSDDKIIKHMARKTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDIPDRH"
     gene            19131..20321
                     /gene="ydeA"
                     /locus_tag="ACHKA1_000018"
     CDS             19131..20321
                     /gene="ydeA"
                     /locus_tag="ACHKA1_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO5677738.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20346..21011)
                     /gene="marC"
                     /locus_tag="ACHKA1_000019"
     CDS             complement(20346..21011)
                     /gene="marC"
                     /locus_tag="ACHKA1_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO5677739.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            21223..21657
                     /gene="marR"
                     /locus_tag="ACHKA1_000020"
     CDS             21223..21657
                     /gene="marR"
                     /locus_tag="ACHKA1_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO5677740.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21678..22061
                     /gene="marA"
                     /locus_tag="ACHKA1_000021"
     CDS             21678..22061
                     /gene="marA"
                     /locus_tag="ACHKA1_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO5677741.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            22093..22311
                     /gene="marB"
                     /locus_tag="ACHKA1_000022"
     CDS             22093..22311
                     /gene="marB"
                     /locus_tag="ACHKA1_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO5677742.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(22342..23241)
                     /gene="eamA"
                     /locus_tag="ACHKA1_000023"
     CDS             complement(22342..23241)
                     /gene="eamA"
                     /locus_tag="ACHKA1_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO5677743.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGALLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            23436..24623
                     /gene="ydeE"
                     /locus_tag="ACHKA1_000024"
     CDS             23436..24623
                     /gene="ydeE"
                     /locus_tag="ACHKA1_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO5677744.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVDSVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGLVLTNLPPSSLFIILALVIIAAWVLMLKGIRARPWRQP
                     ALC"
     gene            24750..24845
                     /gene="mgtS"
                     /locus_tag="ACHKA1_000025"
     CDS             24750..24845
                     /gene="mgtS"
                     /locus_tag="ACHKA1_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO5677745.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            24845..24950
                     /gene="mgtT"
                     /locus_tag="ACHKA1_000026"
                     /pseudo
     CDS             24845..24950
                     /gene="mgtT"
                     /locus_tag="ACHKA1_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
     gene            complement(25065..25880)
                     /gene="dgcZ"
                     /locus_tag="ACHKA1_000027"
                     /pseudo
     CDS             complement(25065..25880)
                     /gene="dgcZ"
                     /locus_tag="ACHKA1_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
     gene            complement(26128..26520)
                     /gene="ydeI"
                     /locus_tag="ACHKA1_000028"
     CDS             complement(26128..26520)
                     /gene="ydeI"
                     /locus_tag="ACHKA1_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO5677746.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            26796..27314
                     /gene="ydeJ"
                     /locus_tag="ACHKA1_000029"
     CDS             26796..27314
                     /gene="ydeJ"
                     /locus_tag="ACHKA1_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO5677747.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(27359..29404)
                     /gene="dcp"
                     /locus_tag="ACHKA1_000030"
     CDS             complement(27359..29404)
                     /gene="dcp"
                     /locus_tag="ACHKA1_000030"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO5677748.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMLQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGSLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWGFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDEVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            29541..30287
                     /gene="ydfG"
                     /locus_tag="ACHKA1_000031"
     CDS             29541..30287
                     /gene="ydfG"
                     /locus_tag="ACHKA1_000031"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO5677749.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            30376..31062
                     /gene="rspR"
                     /locus_tag="ACHKA1_000032"
                     /gene_synonym="ydfH"
     CDS             30376..31062
                     /gene="rspR"
                     /locus_tag="ACHKA1_000032"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO5677750.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            31239..31442
                     /gene="ydfZ"
                     /locus_tag="ACHKA1_000033"
     CDS             31239..31442
                     /gene="ydfZ"
                     /locus_tag="ACHKA1_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO5677751.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(31477..32937)
                     /gene="ydfI"
                     /locus_tag="ACHKA1_000034"
     CDS             complement(31477..32937)
                     /gene="ydfI"
                     /locus_tag="ACHKA1_000034"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO5677752.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLFLLAHGAKATVAKYSVK"
     gene            complement(33027..34394)
                     /gene="ydfJ"
                     /locus_tag="ACHKA1_000035"
     CDS             complement(33027..34394)
                     /gene="ydfJ"
                     /locus_tag="ACHKA1_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFO5677753.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQ
                     PSRSL"
     gene            complement(34452..35471)
                     /gene="rspB"
                     /locus_tag="ACHKA1_000036"
     CDS             complement(34452..35471)
                     /gene="rspB"
                     /locus_tag="ACHKA1_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO5677754.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHVDGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWVINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(35483..36697)
                     /gene="rspA"
                     /locus_tag="ACHKA1_000037"
     CDS             complement(35483..36697)
                     /gene="rspA"
                     /locus_tag="ACHKA1_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO5677755.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTITPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(36903..37229)
                     /gene="ynfA"
                     /locus_tag="ACHKA1_000038"
     CDS             complement(36903..37229)
                     /gene="ynfA"
                     /locus_tag="ACHKA1_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO5677756.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            37364..37705
                     /gene="ynfB"
                     /locus_tag="ACHKA1_000039"
     CDS             37364..37705
                     /gene="ynfB"
                     /locus_tag="ACHKA1_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO5677757.1"
                     /translation="MKITLSKRIGLLAFLLPCTLALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            37740..38300
                     /gene="speG"
                     /locus_tag="ACHKA1_000040"
     CDS             37740..38300
                     /gene="speG"
                     /locus_tag="ACHKA1_000040"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO5677758.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(38303..39013)
                     /gene="ynfC"
                     /locus_tag="ACHKA1_000041"
     CDS             complement(38303..39013)
                     /gene="ynfC"
                     /locus_tag="ACHKA1_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO5677759.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            39121..39426
                     /gene="ynfD"
                     /locus_tag="ACHKA1_000042"
     CDS             39121..39426
                     /gene="ynfD"
                     /locus_tag="ACHKA1_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO5677760.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            39624..42050
                     /gene="ynfE"
                     /locus_tag="ACHKA1_000043"
     CDS             39624..42050
                     /gene="ynfE"
                     /locus_tag="ACHKA1_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO5677761.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            42111..44534
                     /gene="ynfF"
                     /locus_tag="ACHKA1_000044"
     CDS             42111..44534
                     /gene="ynfF"
                     /locus_tag="ACHKA1_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO5677762.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPKMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            44545..45162
                     /gene="ynfG"
                     /locus_tag="ACHKA1_000045"
     CDS             44545..45162
                     /gene="ynfG"
                     /locus_tag="ACHKA1_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO5677763.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            45164..46018
                     /gene="ynfH"
                     /locus_tag="ACHKA1_000046"
     CDS             45164..46018
                     /gene="ynfH"
                     /locus_tag="ACHKA1_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO5677764.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            46061..46675
                     /gene="dmsD"
                     /locus_tag="ACHKA1_000047"
     CDS             46061..46675
                     /gene="dmsD"
                     /locus_tag="ACHKA1_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO5677765.1"
                     /translation="MTHFSQQDNFSVVARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGKLARLTLAQWQSQLLIPVAVKPLFR"
     gene            46836..48128
                     /gene="clcB"
                     /locus_tag="ACHKA1_000048"
     CDS             46836..48128
                     /gene="clcB"
                     /locus_tag="ACHKA1_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO5677766.1"
                     /translation="MHHLHIYPNLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(48081..48776)
                     /gene="bioD"
                     /locus_tag="ACHKA1_000049"
     CDS             complement(48081..48776)
                     /gene="bioD"
                     /locus_tag="ACHKA1_000049"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO5677767.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(48901..50121)
                     /gene="mlc"
                     /locus_tag="ACHKA1_000050"
                     /gene_synonym="dgsA"
     CDS             complement(48901..50121)
                     /gene="mlc"
                     /locus_tag="ACHKA1_000050"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO5677768.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(50256..51149)
                     /gene="ynfL"
                     /locus_tag="ACHKA1_000051"
     CDS             complement(50256..51149)
                     /gene="ynfL"
                     /locus_tag="ACHKA1_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5677769.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            51256..52509
                     /gene="ynfM"
                     /locus_tag="ACHKA1_000052"
     CDS             51256..52509
                     /gene="ynfM"
                     /locus_tag="ACHKA1_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5677770.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(52558..52677)
                     /locus_tag="ACHKA1_000053"
     CDS             complement(52558..52677)
                     /locus_tag="ACHKA1_000053"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO5677771.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            52933..53241
                     /gene="asr"
                     /locus_tag="ACHKA1_000054"
     CDS             52933..53241
                     /gene="asr"
                     /locus_tag="ACHKA1_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO5677772.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            53334..53417
                     /locus_tag="ACHKA1_000055"
     CDS             53334..53417
                     /locus_tag="ACHKA1_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5677773.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            53517..54338
                     /gene="ydgD"
                     /locus_tag="ACHKA1_000056"
     CDS             53517..54338
                     /gene="ydgD"
                     /locus_tag="ACHKA1_000056"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO5677774.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(54377..54706)
                     /gene="mdtI"
                     /locus_tag="ACHKA1_000057"
     CDS             complement(54377..54706)
                     /gene="mdtI"
                     /locus_tag="ACHKA1_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO5677775.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(54693..55058)
                     /gene="mdtJ"
                     /locus_tag="ACHKA1_000058"
     CDS             complement(54693..55058)
                     /gene="mdtJ"
                     /locus_tag="ACHKA1_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO5677776.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            55470..56504
                     /gene="tqsA"
                     /locus_tag="ACHKA1_000059"
     CDS             55470..56504
                     /gene="tqsA"
                     /locus_tag="ACHKA1_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO5677777.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(56529..57917)
                     /gene="pntB"
                     /locus_tag="ACHKA1_000060"
     CDS             complement(56529..57917)
                     /gene="pntB"
                     /locus_tag="ACHKA1_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO5677778.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(57928..59460)
                     /gene="pntA"
                     /locus_tag="ACHKA1_000061"
     CDS             complement(57928..59460)
                     /gene="pntA"
                     /locus_tag="ACHKA1_000061"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO5677779.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            59984..60928
                     /gene="ydgH"
                     /locus_tag="ACHKA1_000062"
     CDS             59984..60928
                     /gene="ydgH"
                     /locus_tag="ACHKA1_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO5677780.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            61114..62496
                     /gene="ydgI"
                     /locus_tag="ACHKA1_000063"
     CDS             61114..62496
                     /gene="ydgI"
                     /locus_tag="ACHKA1_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5677781.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            62533..63255
                     /gene="folM"
                     /locus_tag="ACHKA1_000064"
     CDS             62533..63255
                     /gene="folM"
                     /locus_tag="ACHKA1_000064"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO5677782.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(63252..63587)
                     /gene="ydgC"
                     /locus_tag="ACHKA1_000065"
     CDS             complement(63252..63587)
                     /gene="ydgC"
                     /locus_tag="ACHKA1_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO5677783.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            63716..64435
                     /gene="rstA"
                     /locus_tag="ACHKA1_000066"
     CDS             63716..64435
                     /gene="rstA"
                     /locus_tag="ACHKA1_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO5677784.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            64439..65740
                     /gene="rstB"
                     /locus_tag="ACHKA1_000067"
     CDS             64439..65740
                     /gene="rstB"
                     /locus_tag="ACHKA1_000067"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO5677785.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            65816..66745
                     /gene="tus"
                     /locus_tag="ACHKA1_000068"
     CDS             65816..66745
                     /gene="tus"
                     /locus_tag="ACHKA1_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO5677786.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHNPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(66742..68145)
                     /gene="fumC"
                     /locus_tag="ACHKA1_000069"
     CDS             complement(66742..68145)
                     /gene="fumC"
                     /locus_tag="ACHKA1_000069"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO5677787.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLNEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(68288..69934)
                     /gene="fumB"
                     /locus_tag="ACHKA1_000070"
     CDS             complement(68288..69934)
                     /gene="fumB"
                     /locus_tag="ACHKA1_000070"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5677788.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            70133..71308
                     /gene="manA"
                     /locus_tag="ACHKA1_000071"
     CDS             70133..71308
                     /gene="manA"
                     /locus_tag="ACHKA1_000071"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO5677789.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIL
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            71409..72917
                     /gene="ydgA"
                     /locus_tag="ACHKA1_000072"
     CDS             71409..72917
                     /gene="ydgA"
                     /locus_tag="ACHKA1_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO5677790.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEEMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(72962..74227)
                     /gene="uidC"
                     /locus_tag="ACHKA1_000073"
     CDS             complement(72962..74227)
                     /gene="uidC"
                     /locus_tag="ACHKA1_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO5677791.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(74266..75639)
                     /gene="uidB"
                     /locus_tag="ACHKA1_000074"
     CDS             complement(74266..75639)
                     /gene="uidB"
                     /locus_tag="ACHKA1_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO5677792.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(75636..77447)
                     /gene="uidA"
                     /locus_tag="ACHKA1_000075"
     CDS             complement(75636..77447)
                     /gene="uidA"
                     /locus_tag="ACHKA1_000075"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO5677793.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNYASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(77838..78428)
                     /gene="uidR"
                     /locus_tag="ACHKA1_000076"
     CDS             complement(77838..78428)
                     /gene="uidR"
                     /locus_tag="ACHKA1_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO5677794.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(78657..79424)
                     /gene="hdhA"
                     /locus_tag="ACHKA1_000077"
     CDS             complement(78657..79424)
                     /gene="hdhA"
                     /locus_tag="ACHKA1_000077"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO5677795.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(79536..80564)
                     /gene="malI"
                     /locus_tag="ACHKA1_000078"
     CDS             complement(79536..80564)
                     /gene="malI"
                     /locus_tag="ACHKA1_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO5677796.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            80739..82331
                     /gene="malX"
                     /locus_tag="ACHKA1_000079"
     CDS             80739..82331
                     /gene="malX"
                     /locus_tag="ACHKA1_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO5677797.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGIVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            82341..83513
                     /gene="malY"
                     /locus_tag="ACHKA1_000080"
     CDS             82341..83513
                     /gene="malY"
                     /locus_tag="ACHKA1_000080"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO5677798.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNATFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            83617..84618
                     /gene="add"
                     /locus_tag="ACHKA1_000081"
     CDS             83617..84618
                     /gene="add"
                     /locus_tag="ACHKA1_000081"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO5677799.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRSQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLDHGIRASIN
                     TDDPGVQGVDIIHEYNVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(84653..85693)
                     /gene="ydgJ"
                     /locus_tag="ACHKA1_000082"
     CDS             complement(84653..85693)
                     /gene="ydgJ"
                     /locus_tag="ACHKA1_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO5677800.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKSGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            85936..86061
                     /gene="blr"
                     /locus_tag="ACHKA1_000083"
     CDS             85936..86061
                     /gene="blr"
                     /locus_tag="ACHKA1_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO5677801.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            86334..86549
                     /gene="ydgT"
                     /locus_tag="ACHKA1_000084"
     CDS             86334..86549
                     /gene="ydgT"
                     /locus_tag="ACHKA1_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO5677802.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            86635..87075
                     /gene="ydgK"
                     /locus_tag="ACHKA1_000085"
     CDS             86635..87075
                     /gene="ydgK"
                     /locus_tag="ACHKA1_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO5677803.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            87152..87733
                     /gene="rsxA"
                     /locus_tag="ACHKA1_000086"
     CDS             87152..87733
                     /gene="rsxA"
                     /locus_tag="ACHKA1_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO5677804.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            87733..88311
                     /gene="rsxB"
                     /locus_tag="ACHKA1_000087"
     CDS             87733..88311
                     /gene="rsxB"
                     /locus_tag="ACHKA1_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO5677805.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            88304..90430
                     /gene="rsxC"
                     /locus_tag="ACHKA1_000088"
     CDS             88304..90430
                     /gene="rsxC"
                     /locus_tag="ACHKA1_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO5677806.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAISRA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEEPVDPRKAAVE
                     AAIARAKARKLEQQPANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAVPEEQVDP
                     RKAAVAAAIARVQAKKAAQQKVVNED"
     gene            90431..91489
                     /gene="rsxD"
                     /locus_tag="ACHKA1_000089"
     CDS             90431..91489
                     /gene="rsxD"
                     /locus_tag="ACHKA1_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO5677807.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGMLAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLTLCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            91493..92113
                     /gene="rsxG"
                     /locus_tag="ACHKA1_000090"
     CDS             91493..92113
                     /gene="rsxG"
                     /locus_tag="ACHKA1_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO5677808.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            92117..92812
                     /gene="rsxE"
                     /locus_tag="ACHKA1_000091"
     CDS             92117..92812
                     /gene="rsxE"
                     /locus_tag="ACHKA1_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO5677809.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADSLLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            92812..93447
                     /gene="nth"
                     /locus_tag="ACHKA1_000092"
     CDS             92812..93447
                     /gene="nth"
                     /locus_tag="ACHKA1_000092"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO5677810.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            94057..95559
                     /gene="dtpA"
                     /locus_tag="ACHKA1_000093"
     CDS             94057..95559
                     /gene="dtpA"
                     /locus_tag="ACHKA1_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO5677811.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
     gene            95665..96270
                     /gene="gstA"
                     /locus_tag="ACHKA1_000094"
     CDS             95665..96270
                     /gene="gstA"
                     /locus_tag="ACHKA1_000094"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO5677812.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(96314..97177)
                     /gene="pdxY"
                     /locus_tag="ACHKA1_000095"
     CDS             complement(96314..97177)
                     /gene="pdxY"
                     /locus_tag="ACHKA1_000095"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO5677813.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(97236..98510)
                     /gene="tyrS"
                     /locus_tag="ACHKA1_000096"
     CDS             complement(97236..98510)
                     /gene="tyrS"
                     /locus_tag="ACHKA1_000096"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO5677814.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(98639..99295)
                     /gene="pdxH"
                     /locus_tag="ACHKA1_000097"
     CDS             complement(98639..99295)
                     /gene="pdxH"
                     /locus_tag="ACHKA1_000097"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO5677815.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(99354..99683)
                     /gene="mliC"
                     /locus_tag="ACHKA1_000098"
     CDS             complement(99354..99683)
                     /gene="mliC"
                     /locus_tag="ACHKA1_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO5677816.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNSRR"
     gene            complement(99781..100890)
                     /gene="anmK"
                     /locus_tag="ACHKA1_000099"
     CDS             complement(99781..100890)
                     /gene="anmK"
                     /locus_tag="ACHKA1_000099"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO5677817.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            101164..101631
                     /gene="slyB"
                     /locus_tag="ACHKA1_000100"
     CDS             101164..101631
                     /gene="slyB"
                     /locus_tag="ACHKA1_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO5677818.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(101678..102112)
                     /gene="slyA"
                     /locus_tag="ACHKA1_000101"
     CDS             complement(101678..102112)
                     /gene="slyA"
                     /locus_tag="ACHKA1_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO5677819.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            102313..102549
                     /gene="ydhI"
                     /locus_tag="ACHKA1_000102"
     CDS             102313..102549
                     /gene="ydhI"
                     /locus_tag="ACHKA1_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO5677820.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            102552..103409
                     /gene="ydhJ"
                     /locus_tag="ACHKA1_000103"
     CDS             102552..103409
                     /gene="ydhJ"
                     /locus_tag="ACHKA1_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO5677821.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVFSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            103409..105421
                     /gene="ydhK"
                     /locus_tag="ACHKA1_000104"
     CDS             103409..105421
                     /gene="ydhK"
                     /locus_tag="ACHKA1_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO5677822.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(105422..105943)
                     /gene="sodC"
                     /locus_tag="ACHKA1_000105"
     CDS             complement(105422..105943)
                     /gene="sodC"
                     /locus_tag="ACHKA1_000105"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO5677823.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(106024..106920)
                     /gene="ydhF"
                     /locus_tag="ACHKA1_000106"
     CDS             complement(106024..106920)
                     /gene="ydhF"
                     /locus_tag="ACHKA1_000106"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO5677824.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(106969..107208)
                     /gene="ydhL"
                     /locus_tag="ACHKA1_000107"
     CDS             complement(106969..107208)
                     /gene="ydhL"
                     /locus_tag="ACHKA1_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO5677825.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            107311..107910
                     /gene="nemR"
                     /locus_tag="ACHKA1_000108"
     CDS             107311..107910
                     /gene="nemR"
                     /locus_tag="ACHKA1_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO5677826.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRNSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKTIIATSAV"
     gene            107948..109045
                     /gene="nemA"
                     /locus_tag="ACHKA1_000109"
     CDS             107948..109045
                     /gene="nemA"
                     /locus_tag="ACHKA1_000109"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO5677827.1"
                     /translation="MSSEKLYSPLQVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSQEQIAAWKKITAGVHAENGHIAVQL
                     WHTGRISHASLQPDGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRTRFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     TRLQRKAGLNPQRAESFYGGGAEGYTDYPTL"
     gene            109126..109533
                     /gene="gloA"
                     /locus_tag="ACHKA1_000110"
     CDS             109126..109533
                     /gene="gloA"
                     /locus_tag="ACHKA1_000110"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO5677828.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            109636..110283
                     /gene="rnt"
                     /locus_tag="ACHKA1_000111"
     CDS             109636..110283
                     /gene="rnt"
                     /locus_tag="ACHKA1_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO5677829.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            110376..114992
                     /gene="lhr"
                     /locus_tag="ACHKA1_000112"
     CDS             110376..114992
                     /gene="lhr"
                     /locus_tag="ACHKA1_000112"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO5677830.1"
                     /translation="MADNPNLSSPLPDVFSPATRDWFLSAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDVAHFE
                     STSGATSNRVQSSDIFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQHTVAAAAMDALQVDEWYSRVRRATPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILSARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRTSQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLTVKRIFPAMISGQERLACMDDAARLRDALGVQLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(115043..115390)
                     /gene="grxD"
                     /locus_tag="ACHKA1_000113"
     CDS             complement(115043..115390)
                     /gene="grxD"
                     /locus_tag="ACHKA1_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO5677831.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            115725..116552
                     /gene="mepH"
                     /locus_tag="ACHKA1_000114"
     CDS             115725..116552
                     /gene="mepH"
                     /locus_tag="ACHKA1_000114"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO5677832.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            116680..117261
                     /gene="sodB"
                     /locus_tag="ACHKA1_000115"
     CDS             116680..117261
                     /gene="sodB"
                     /locus_tag="ACHKA1_000115"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO5677833.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(117407..118576)
                     /gene="ydhP"
                     /locus_tag="ACHKA1_000116"
     CDS             complement(117407..118576)
                     /gene="ydhP"
                     /locus_tag="ACHKA1_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5677834.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(118742..118831)
                     /gene="cydH"
                     /locus_tag="ACHKA1_000117"
                     /gene_synonym="ynhF"
     CDS             complement(118742..118831)
                     /gene="cydH"
                     /locus_tag="ACHKA1_000117"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO5677835.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            119130..120155
                     /gene="purR"
                     /locus_tag="ACHKA1_000118"
     CDS             119130..120155
                     /gene="purR"
                     /locus_tag="ACHKA1_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO5677836.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(120152..121084)
                     /gene="punR"
                     /locus_tag="ACHKA1_000119"
     CDS             complement(120152..121084)
                     /gene="punR"
                     /locus_tag="ACHKA1_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO5677837.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            121197..122408
                     /gene="punC"
                     /locus_tag="ACHKA1_000120"
     CDS             121197..122408
                     /gene="punC"
                     /locus_tag="ACHKA1_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO5677838.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAAMLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            122699..123847
                     /gene="cfa"
                     /locus_tag="ACHKA1_000121"
     CDS             122699..123847
                     /gene="cfa"
                     /locus_tag="ACHKA1_000121"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO5677839.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(123887..124528)
                     /gene="ribE"
                     /locus_tag="ACHKA1_000122"
     CDS             complement(123887..124528)
                     /gene="ribE"
                     /locus_tag="ACHKA1_000122"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO5677840.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            124743..126116
                     /gene="mdtK"
                     /locus_tag="ACHKA1_000123"
     CDS             124743..126116
                     /gene="mdtK"
                     /locus_tag="ACHKA1_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO5677841.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(126157..127413)
                     /gene="ydhQ"
                     /locus_tag="ACHKA1_000124"
     CDS             complement(126157..127413)
                     /gene="ydhQ"
                     /locus_tag="ACHKA1_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO5677842.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIIENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            127721..127797
                     /locus_tag="ACHKA1_000125"
     tRNA            127721..127797
                     /locus_tag="ACHKA1_000125"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:127755..127757,aa:Val,seq:gac)
     gene            127802..127878
                     /locus_tag="ACHKA1_000126"
     tRNA            127802..127878
                     /locus_tag="ACHKA1_000126"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:127836..127838,aa:Val,seq:gac)
     gene            127986..128291
                     /gene="ydhR"
                     /locus_tag="ACHKA1_000127"
     CDS             127986..128291
                     /gene="ydhR"
                     /locus_tag="ACHKA1_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO5677843.1"
                     /translation="MATLLQLHFSFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            128417..130021
                     /gene="ydhS"
                     /locus_tag="ACHKA1_000128"
     CDS             128417..130021
                     /gene="ydhS"
                     /locus_tag="ACHKA1_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO5677844.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADVAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEAIRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(130033..130845)
                     /gene="ydhT"
                     /locus_tag="ACHKA1_000129"
     CDS             complement(130033..130845)
                     /gene="ydhT"
                     /locus_tag="ACHKA1_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO5677845.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARIAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(130849..131634)
                     /gene="phsC"
                     /locus_tag="ACHKA1_000130"
     CDS             complement(130849..131634)
                     /gene="phsC"
                     /locus_tag="ACHKA1_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO5677846.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(131631..132299)
                     /gene="ydhX"
                     /locus_tag="ACHKA1_000131"
     CDS             complement(131631..132299)
                     /gene="ydhX"
                     /locus_tag="ACHKA1_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO5677847.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(132363..133001)
                     /gene="ydhW"
                     /locus_tag="ACHKA1_000132"
     CDS             complement(132363..133001)
                     /gene="ydhW"
                     /locus_tag="ACHKA1_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO5677848.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(133014..135116)
                     /gene="ydhV"
                     /locus_tag="ACHKA1_000133"
     CDS             complement(133014..135116)
                     /gene="ydhV"
                     /locus_tag="ACHKA1_000133"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO5677849.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(135137..135763)
                     /gene="ydhY"
                     /locus_tag="ACHKA1_000134"
     CDS             complement(135137..135763)
                     /gene="ydhY"
                     /locus_tag="ACHKA1_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO5677850.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLTGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT        33603 a        34977 c        33157 g        34249 t
ORIGIN      
        1 attaaacccc ttttgcatcc attaatgtct ttaggaatat tcgctataaa ataagggtta
       61 tgcttaatgc tttaagaaaa atagcaactt tcccttgaat atagtactgg tgatggaacg
      121 atgaatctgc aagctggcat ttaacaagcc agctctaaaa gaagggaaat aagaaaaact
      181 atactcaaaa actaacagcc acggtcatca tgatgtggct gtcaatgaaa ctataaccca
      241 ggcgctttcc ataacgatgt cgtcagtcca tgatcaacca gccccagctg atcctgataa
      301 actgcctgcc acttatcgcg tgaatcctga taaagttcat gcttttccgg gtctggtgtg
      361 tgcgtccgtt cccagcgaac caggcgttct ccggtttctg ccattgatga aaaaattccg
      421 gcaccgacgc cagctgcaat ggcacatcct aatgcggtgg cttctttgac caccggaata
      481 ttgacgggta atcccgagac atcagcgaga atttgactcc ataatttccc ttttgaacct
      541 ccgcctgcaa agactaacga tgaaggatga atattcgaga aatcagcaat ttgctgcaag
      601 ttacacgctg atacaatcgc cgcattttct tccagcgcac ggaacaatgt cgctttgtta
      661 catttatccg ggtcaatgga caagttaata aaggaaggcg cagcgtgata ccaggtttta
      721 aagcgcattc tgtcggagaa gatcggcatt acgccccacg acccaggcgg cacccgactg
      781 gccatctctt ccagcagcgt ataggtgtcg atgcctaaac gttcggcaat cagtttttct
      841 tcggcacaga aagcatcgcg gaaccagcgc atggtgagtc cggtaaaaaa gcttatagat
      901 tcagcttgta ccatgccagg aataacatga ggattaacgc gcacgttcat ttctgggtct
      961 gtcaccggcg cggctaaatt tacaacttgc tgccagaatg tgccgccaag aaccgcggtt
     1021 tgtgccggac gcacaacgcc taaccccagg caaccaagct gcacgtcgcc tcctccaacg
     1081 accaccggag tgcccacctt cagaccgcag agttccgccg cttgtgaact taccacgccc
     1141 agcaatgtgc cggtttcttt gacaggagaa agaatatcgg cacgcaggcc agccatatcc
     1201 agcaatgcag gtttccagtc acggatggtt agatcaagaa gtcccgtggt gccagcgtta
     1261 gagggatcca ccgccagttc gccgctgagc atataggcca gccagtcgct gatcatggtg
     1321 atggttgatg cctgacggta aatatcggaa cgatggtgcg ccagccaaag taatctgggg
     1381 atggcactta aagccagtgt ttgtccggtc gcgcgataaa cttcgttttc aaaggtattg
     1441 ttgtgcagtt ctttaagttc gctaacttcg cgcgctgctc tggcatccac attggcgcag
     1501 gcccagatcg gtgttccttc attgttatat aaaacaatgc cttcacgcat agaacatgcc
     1561 gaaacggcag cgatatactc cgggtctatg ccggcgttgt gcagcgcctg gcgcatacac
     1621 tcacacgcca gttgccagtt tttgttgaga tcaaattcca tagaaccagg aacgtccggt
     1681 actgccagat gccgccactc cgcctgtccc actgctattt gattgccttc caggtcgaat
     1741 atcacagccc gaatacttcc ggtgcctgca tccagcgcca tcaggtagta ctttgattct
     1801 gaaggggtaa agagtcgagc cattagatta tcctcggctt atgcgttctg tctgttcctc
     1861 tatacgttct ccatcattcc cggtaataag gtcatgcaaa tttaactacg taaaatcgcc
     1921 gctgctgtgt cctgatcggt aaccagtgcg ttgatataac cgcctttcat tgcagcggca
     1981 attgcttcgg ctttattttc tccccctgcc acgccaaccc ggacgggtat ggtcttcagc
     2041 gcgcttaaag gtaagccaat cagttcgtta tgtattttga tatccgtgac aacgtcacct
     2101 tttgcatcaa aaaagtagcc taaaatgtcg ccaaccgccc cttttcggcc aatcattaac
     2161 tgttcgccct ggctgatata accggagcga atgatcgtcg cgtcgtcttg ttgactcacg
     2221 gccccaatgc cgacaatcgc cacatccgct gcttgcgcgg ctaacagaac atctttgacg
     2281 caattttcat tttttagcgt acgggcaatg tcagcggagg atgcccgcaa cggagccgga
     2341 ataatattca cactgcacgc cgcgttaagc tgcccgatac ccgtcatata ggaaccgacg
     2401 ccaccggaga gcgtgaccag gcgaatttgc tgtgacgaaa taaaaccact taagcgttgc
     2461 agcgtattca tggttgcctc gccaaaacca atcgccagca tctgttgtgg ttgaagtaaa
     2521 ctcatcaaca tatgcgccgc gcctatcccc agtcgcccgc cgacatcagc atccgcaagc
     2581 ccagggatca cccggacatg ttgcagcgaa aactgacgac gtaattgtgt ttcatattcc
     2641 agacagcctt caaagcgaga attaatctgc acgcgaatga tgccggactg atgccctttc
     2701 tccagtaatc gcgacacttt caaacgggtc aggccgagac gatcgctgat ctcactctgg
     2761 gtcagcccgt cgtggtagta aaaccacgcg atccgcgcga cctgttcttc ttcacacatt
     2821 ccctgttctg aaattgccga atcgttgatt gtcataattt attcttcact ttgaacatat
     2881 ttaaatcttt aatgcaattg ttcagttctt gcgcatttat atctgtgatg gcaaccacag
     2941 tttgactcta cgagcatgaa caaacgcaac cgtgaaaatc aaaatagcat aaattgtgat
     3001 ctattcgtcg gaaatatgtg caatgtccac ctaaggttat gaacaaatta aaagcagaaa
     3061 tacatttgtt caaaactcac ctgcaaaact gaacggggga aatatgcaaa cgagtgatac
     3121 ccgcgcgtta ccgctacttt gcgcccgctc ggtttataaa cagtattcag gggttaatgt
     3181 cctgaaaggc atcgatttta cgttgcacca gggggaggtc cacgccctgc tcggcggcaa
     3241 tggtgccggt aaatcgacgt taatgaagat tattgccggt attacccctg ctgatagcgg
     3301 tacgctggag attgggggca acaactacgt cagattaacg ccagttcatg ctcatcagct
     3361 gggtatttat ctcgttcccc aggaaccgct gcttttccca agcctgtcga taaaagaaaa
     3421 catcctgttt gggctggcaa aaaaacagct ctccatgcag aaaatgaaga acttgctggc
     3481 ggcgctgggc tgccagtttg atctgcatag tctggcagga tcgctggatg tcgccgatcg
     3541 ccaaatggtg gaaatcctcc gcgggctgat gcgcgactcg cggattctga tcctcgatga
     3601 acctaccgcc tcgcttaccc ctgcggaaac cgaacgcttg tttagtcgct tgcaagagct
     3661 gcttgctact ggcgtgggta ttgtttttat ctcgcataag ctgccggaaa ttcgccagat
     3721 tgccgatcga attagcgtga tgcgcgacgg aaccatcgcc ttaagcggca aaaccagcga
     3781 actgtctacc gacgacatta ttcaggccat cacgccagtg gtacgggaaa aatcgctctc
     3841 tgccagccaa aaattatggc tggagttacc tggtaaccgc ccacaacatg ccgtaggaac
     3901 gtcggtgctg acactggaaa atctgactgg cgaaggtttt aggaatgtca gcctgacgct
     3961 aaatgctgga gaaattctgg gtctggctgg gctggtgggt gccggacgca cagaactggc
     4021 cgagacgctc tatggtctac gtactttgcg tggcggacgc attatgctga atgataaaga
     4081 gatcaataga ttctccactg gagaacgttt actgcgcggt ctcgtttatc tgccggaaga
     4141 tcgccagtcc tccggactga atctcgatgc ttcgctggcc tggaacgtct gcgcccttac
     4201 tcataacctt cgtggattct gggcgaaaac cgcgaaagat aatgccaccc tggaacgata
     4261 tcgtcgggcg ctgaatatta aatttaacca accggaacaa gctgcacgga cattatccgg
     4321 tggcaaccag caaaaaatcc tgattgccaa atgcctggaa gcctcgccgc aagtattgat
     4381 agtcgatgag ccgacgcgcg gcgtggatgt ctcggcccgt aatgatatct accagctgtt
     4441 acgcagcatc gccgcacaaa atgtggctgt gctgcttatc tcctccgacc tggaagagat
     4501 cgaactgatg gcagatcgtg tgtatgtgat gcatcagggc gaaattaccc actctgcact
     4561 gaccgggcgc gatattaatg tcgagactat tatgcgcgtt gccttcggcg atagtcagcg
     4621 tcaggaggcg tcatgctgaa gtttattcag aacaaccgtg aaatcacggc actgctggcg
     4681 gtggtgctgc tgtttgtatt acccggtttt ctcgaccgcc agtatttaag tgtgcaaacg
     4741 ctgaccatgg tttatagcag cgcgcaaatc ctgatcctgc tggcaatggg cgcgacgctg
     4801 gtaatgctta cgcgcaatat tgatgtttca gtgggttcga ttaccggaat gtgcgcggtg
     4861 ctgttgggga tgttactgaa cgcggggtat tcactacctg ttgcttgtgt cgcgacttta
     4921 ctgcttggtt tgctcgcagg atttttcaac ggtgtcctgg tcgcgtggct aaaaatccct
     4981 gccattgttg ccacccttgg cacgttaggg ttgtacagag gcatcatgtt gctgtggact
     5041 ggcggtaaat ggattgaggg gttacccgcc gaactgaaac agctctccgc cccgctgctg
     5101 tttggcgttt ccgcgattgg ttggttgacg ataattctgg tggcatttat ggcctggctg
     5161 ttggcaaaga cggcgtttgg acgcagtttt tatgccacgg gcgataattt acagggcgct
     5221 cgtcaactgg gcgttcgtac ggaagccatt cgcattgtgg cattttcgtt gaacggctgc
     5281 atggcggcac tggcgggaat tgtgtttgct tcgcagattg gttttatccc caaccagacc
     5341 ggtaccgggc tggaaatgaa agcaattgca gcctgcgtgc tgggcggtat tagtttgctc
     5401 ggcggttccg gtgcgatcat cggtgcggta ctcggcgcat ggttcctgac gcagatcgat
     5461 agcgtactgg tgctgttgcg cattccggca tggtggaatg attttatcgc gggtctggtt
     5521 ctgctggcgg tgctggtgtt tgatggacgc ctgcgttgtg cgctggaacg taatctgcgg
     5581 cggcaaaaat atgcccgctt tatgacgcca ccgccatccg ttaaaactgc ttcgtcaggt
     5641 aaaaaacggg aggctgcata atgcgtattc gctacggttg ggaactggct cttgccgcac
     5701 tgctcgttat tgagattgtc tcatttggtg caattaaccc gcggatgtta gatctcaata
     5761 tgttgctgtt cagcaccagt gactttatct gcattggcat tgtcgcccta ccgctgacga
     5821 tggtgattgt cagtggcggg atcgatattt cgtttggttc gaccatcggc ctctgcgcca
     5881 ttgcattggg cgtactgttt caaagtggtg tgccgatgcc gctggcgatt ctcctgagct
     5941 tactgctcgg cgcattgtgc gggctgatca acgccggatt aattatctat accaaagtta
     6001 acccgctggt gattacgctt ggcacgctgt atctgtttgc cggaagtgcc ctgctgcttt
     6061 ccgggatggc cggagcgacg gggtacgaag gtattggtgg attcccgatg gcgtttacag
     6121 atttcgctaa cctggatgtg cttggactcc ccgttccgct gattatcttt ctgatatgtc
     6181 tcctcgtttt ctggctctgg ctgcataaaa cccatgccgg acgtaacgtg tttttgattg
     6241 ggcaaagccc gcgcgtggcg ctttatagcg cgattccagt taaccgcacc ttatgtgcgc
     6301 tctatgccat gacggggctg gcgtctgcgg tcgccgctgt gctgctggtg tcgtattttg
     6361 gttcagcacg ttccgatctc ggtgcgtcgt ttctgatgcc cgccatcacc gccgtggtgc
     6421 ttggcggggc caatatttat ggtggttccg gttccattat cggcaccgcc attgcggttt
     6481 tattagtggg atatttgcaa caaggtttgc aaatggcagg agtgccaaat caggtgtcca
     6541 gcgccctttc cggtgcgcta cttatcgtcg ttgtcgtagg tcgttccgtt agcctgcatc
     6601 gccagcaaat taaagagtgg ctggcgcgtc gggccaataa cccattgcca taaaggatat
     6661 cttcatgaca cttcatcgct ttaagaaaat cgccttactt agcgctcttg gcattgccgc
     6721 aatctctatg aatgtgcagg ccgcagagcg tattgcattt attcccaaac tggttggcgt
     6781 gggatttttt accagcggtg gcaacggcgc acaacaagcg ggtaaagagc tgggcgttga
     6841 tgtgacctac gacgggccga cagaacccag tgtttctggt caggtacagt tgattaataa
     6901 cttcgtcaat cagggttata acgccattat cgtttctgcg gtttcgcctg atggcttgtg
     6961 tccggcactg aaacgcgcca tgcaacgtgg tgtgagagtg ctgacctggg actctgatac
     7021 taaaccggag tgccgctctt actacattaa tcagggaacg cccgcccagt tgggaggtat
     7081 gttggtggat atggcggcgc gtcaggtgaa taaagacaaa gccaaagtcg cgtttttcta
     7141 ctcaagcccc accgttacgg accaaaacca gtgggtgaaa gaagcgaaag cgaaaatcgc
     7201 caaagagcat cctggctggg aaattgtcac tacgcagttt ggctataacg atgccactaa
     7261 atcattacaa accgcagaag gaatattaaa agcgtatagc gatctcgacg ccattatcgc
     7321 ccccgatgcc aacgccctgc ccgctgccgc acaagccgca gaaaacctga aaaatgacaa
     7381 agttgcgatt gtcggattca gtacgccaaa cgtgatgcgt ccatatgtgg aacgcggcac
     7441 ggtgaaagaa tttggcctgt gggatgtggt tcagcaaggc aaaatttcag tttatgtcgc
     7501 ggatgcatta ttgaaaaaag gatcaatgaa aacgggcgac aagctggata ttcagggcgt
     7561 aggtcaggtg gaagtctcgc caaacagcgt ccagggctat gactacgaag cggatggtaa
     7621 tggcatcgta ctgttaccgg agcgcgtgat attcaacaaa gagaatatcg gcaaatacga
     7681 tttctgatgt gcattactta accggagtaa gttatggcag atttagacga tattaaagat
     7741 ggtaaagatt ttcgtaccga tcaaccgcaa caaaatatcc cttttaccct gaaaggttgc
     7801 ggtgcgctgg attggggaat gcagtcacgc ttatcgcgga tatttaatcc gaaaacgggt
     7861 aaaaccgtga tgctggcttt tgaccatggt tattttcagg gaccgactac cggacttgaa
     7921 cgcattgata taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc
     7981 ggtattttgc gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca
     8041 ggtgcgaact ctatcctggc ggaattaagc aatgaagccg tggcgttatc gatggatgac
     8101 gccgtgcgcc tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa
     8161 catcagtcga tcaaaaatat tattcagctg gttgatgccg gaatgaaagt gggaatgccg
     8221 accatggccg tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc
     8281 gcgactcgaa tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa
     8341 ggttttgaac ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa
     8401 ttaccggagc gcgaggcgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt
     8461 gtggatatgg ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta
     8521 caggcggtgg ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa
     8581 aaacagtaac tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg
     8641 ttcatgaaga caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg
     8701 tacaggaaga aggcaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct
     8761 tttatatcta cgaagcctat aaagatgaag acgcagtggc gttccataaa accacgcccc
     8821 actacaaaac ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc
     8881 tgttcaatgg gttgatgccg tgaggcgaat ttatcagttt tatctacaat tggggtaacg
     8941 cgctgacggg agtaaataaa tgtctgactg gaacccctct ttatatctgc actttgccgc
     9001 tgaacgatcg cgtccggcgg tggagctgct tgccagagtg ccgctggaga atatcgaata
     9061 tgttgccgat cttggctgtg gcccaggtaa cagcaccgcc ctgctgcatc aacgttggcc
     9121 tgctgccagg ataacgggca tcgactcgtc tccggcgatg attgctgaag cgcgcagtgc
     9181 tttgccagac tgccagttcg tggaagcaga catccgcaac tggcaaccgg aacaggcact
     9241 cgatctgatt tttgctaatg cctcactgca atggctgccc gaccactacg aattgtttcc
     9301 tcacttggtt tctttactta atccgcaggg cgtgctggca gtacagatgc cagataactg
     9361 gctggagccg acccatgtgc tcatgcgcga agttgcctgg gaacaaaact acccggatcg
     9421 cgggcgtgag ccgttggctg gcgttcatgc ttactacgat attttgagcg aagccggatg
     9481 tgaggtcgat atctggcgaa ccacttacta tcaccagatg ccgtcgcacc aggcgattat
     9541 cgattgggta acagccactg gattacgtcc gtggttacag gatctgaccg agagcgaaca
     9601 gcagcttttt cttacgcgct accatcagat gttggaagag cagtatccac tgcaagagaa
     9661 cggacagata ctgctggcat ttccgcgtct gtttattgtt gcccgccgta cggagtaaat
     9721 atcacgtcag ttggtaatgg cgatcgggaa gaatttttgc tggaatttcg gcttcatcgt
     9781 tcatctgtaa gaggtcaatt tcaatagcgt tgcagatggc atccagcggt aaatcattgt
     9841 tttcagtacc gaacggatct tccagttctt ccgccagaca atccagcgaa ataaaagtat
     9901 aggaaatcag cacagagata aaaggcgtca tgtaatgcag gtccacgacc agcgcgaacg
     9961 gcagcatgat acaaaacaga taaacggtac gatgcaaaat cagggtgtag gcaaagggaa
    10021 ttggcgtgta ggcaatgcgc tcgcagcctg ccagaaccgc tgaaatatcg ttaagccgat
    10081 cgttgaggct aataaacagg atatctgaaa gttgtccatt gcgtcgctga accgccaacc
    10141 actcccccat tattaacaag atgcggttag ctggagagtt tgaagccagt acacgctgaa
    10201 gatcttcagt cttgagataa tgagccagca cttccgcctg tggctgtttg cgtaatgtca
    10261 tgcgtaaaca gtgggcgaag gcgatttgca gccgggcaaa ctcccttaca cttgccgaat
    10321 ctggcaatgt cgtttttacc tcgcgcagta acgaccgtga ggcaatcatc aactgccccc
    10381 aaagttttcg cgcttcaacg taacgggcgt aaccggcatt attacgaaaa ccaagaaaaa
    10441 tggcgatggc gacaccgaga atgctgaacg gtgcgagggt gaatttgatg cccagatgcg
    10501 tgtaccaggg cagcatgaaa ataacagcga tagaaaagag aaaattgagt agtaagcgcg
    10561 aggatatctt ggataatact gagccatgcc agacaaaaat acggcgcagc cagtgttgtt
    10621 gtggacgaac aatcatggtt atcttcaggc gtggaaaagt cgccctatta aacgtgatta
    10681 cgatcacatt ctcaagacac gacttacaaa ttacctacaa gcttacaaca ttaacaggcg
    10741 ctgcatgtgg cacctgttaa tgataaaggt atataggatg ttgtaactaa tatagtaaaa
    10801 taagaaaccc ggtcgaaacc gggttcagag tattagcaca acgggcgtac tgcttcgcgc
    10861 atcccttttt cgagaatcgc atccaggtca ttagccacct gctcgaccag accaggcact
    10921 tgcgtcaggt cctgctccca gtggtctttt tctgccaaca caatcgctac cagttcctgg
    10981 gtaccgataa cacggtcacg atgctgactc cagagttgct gataacgttc cagccagtga
    11041 gcatcgtcct gtaccggata tgtttctccg ttacgctcac cacgatagaa cgcaatcaat
    11101 gccgctaatg cgaaagtaag gcgcgccgga agtgtgccgt ttgccttctg ccctgccagt
    11161 agctgcggca ggatgcgggt gcggaacttg gtcataccgt tgagcgcgat agacagcagc
    11221 tgatgcttaa tgtacgggtt acggaaacgc ccggtgactg cactggcgaa agactccagt
    11281 tcatcgcgag gcaaatccag aaccggaata atttcttcgt agatggcttt ttcaacgaat
    11341 gcgcaaattt cagcatcgtt catcgcttca cctacggtat ccagccctgc ctggaacgcc
    11401 accggcacca acgcggtgtg tgcaccgttg agtattgcca ctttgcgttc tttatacggt
    11461 ttaatatcgt caacaatcag cacgttgagc ggatatttgt ccagacgcag ctcggtcgct
    11521 aaggatttcg gtccctgaat cacaaacagg taaaagtgtt cagcggtgtc gaggaaacca
    11581 tcgtgataac ccagttcttc ttccagttta gctacttcat cacgcggata accggtaacg
    11641 atacggtcca ccagcgtaga acagaagctg ttagcctgat ccagccattg aataaatgct
    11701 tctggcagtg cccactcttg cgcatagcgc agcaccagtt cacgcaacgc gtcgccattg
    11761 tagtcgatca actcacacgg gatgatgatc caacctttat ccagcgcacc gttgaaatgg
    11821 ctaaaacgtt cgaacagcag acgggtcagt tttgccggat aacttaccgc tggcgcgtca
    11881 tcgaatttat cgcccgcatg gtagctgata cccgcttcgg tggtgttaga gaaaacaaag
    11941 cgcatttccg ggttgtgcgc cagtttcagg aattcatcgt attcactgta gacgctgatt
    12001 tcacgattaa cagagcgaat cagacgcgcg tcgctgaccg cttccccctt ctcattcagg
    12061 ccacggataa tggtggtgta cagaccatcc tgcgtgctca gtgacggcgg gaatgaagtt
    12121 tcaatcggac gaacaacgac cacaccagaa ttcagatcgg tgtgctcatt caggagatcg
    12181 atttgccagt caacaaaggc gcgcaggaag ttaccttcac caaactgaat gatacgttct
    12241 ggatactgtg caccgggaaa atcgcgacga tttagtgttt tcacaatggg ttcccttctg
    12301 attagtcata caacctgttt gaattggtac gacaggttag caaactttaa tacgccgaac
    12361 ccctgttttg atcaactcct gatgattaat gagcagtttt atgagaaaag tgtggcgcgg
    12421 atcatggttt aatcgaggaa aaacgccttt tcctggatca taaagtggta gaacacattg
    12481 catttaaatc gctctgaatg aacaaagatg tcagacaact tcctcaccgt aacgcatagt
    12541 gctggtacgg ttgcgcccat ctttcttcga gcgatacaga cacgtatcgg cttcgaccat
    12601 caatttatta aagtcatcgg tcagcgttcg gtacgatgcg cgaccactac cgacgccaat
    12661 acttaccgtg agataaagcg ttttttgttg ccaggtgaat ggttgcagtt caacgccttt
    12721 acggattttt tccgccatta gcagaccatc gacaggattc accgacggca ctgcaacagc
    12781 aaattcttcg ccgcccatgc gcgccaccag ccccttatca ccgacaatct tctgaatatg
    12841 ccgggcaaac acgcttaaca ctttatcgcc acattcatgt ccatagttat cgttaatact
    12901 tttgaaatag tcgatatcaa gcagcatgac agtcagatgt tgtgtctgtt tcagcgatgg
    12961 acttttcagc gcctcataaa gaccggaccg tgagtaaacc tgagtcagaa aatcaaagtc
    13021 ggctcgcagc gcaacttgct tcattagcga attgatcgct gccacgctaa aagaaaccat
    13081 aattgggcat atcgccatcg tggcaatacc gagacgtgcg gagaacattt ccggaatgga
    13141 gaacggcgaa ccgaccgaga tatcaatcac cgaattagcc accagtacga tttccaccgc
    13201 accggtgaca aaggtcaaca gacatgttac ctgcggcgta tagcgcactg cacaccagat
    13261 taatgcaggc agcgggaacg ccagactccc cgctccgcca atgactaccg aggcaataac
    13321 cgacacaata agcgcgatgg cgggcatcat ctgttctgct ttaaagcgcg gcagcactcc
    13381 aggaatagcc aacgtcagca tacaaggcac gatcaacacg cccgttgaga attgctcact
    13441 gaaccagtcc gcaagcaaag gccagaaatc cagactatca atactgaccg aaccaatcgc
    13501 cccgacaata gcgcataata atgcggcaat cagacagtaa ttaaatagcc gtaaggcact
    13561 tactggctca tatttatttt tccccaaacg cttatcccgc acgaccagta aggcgacggt
    13621 aacaataaac atcatattgg agaaattgat agccagtgaa accagtcccc attcggtggt
    13681 tatggcatca taaaccagca tcgccacata actgatcgca tagtaatgca gtcgattaag
    13741 ccagacatag cgggcaaaca cccccgccat tacgccattc agcggccaga aaagagaaag
    13801 ttcgtgaacc agccgtaact ctgcaccaat aaaatagaac agcgtggtta gcgcaaaaat
    13861 agcgatgcta ttacgtagca gatgaccttc ctggaacaca cggaaagttg aaatggggtg
    13921 tacatgcatt aattatctgt ccatacaatt gcgggtggca attatctcaa cgtaataagt
    13981 aaattctcat aaaggatagc atgttcgctg acagcggcgg caaataaagg taccctggga
    14041 atgctatttt gtcgttttcc gcaattctca tggctgattc atctgtacaa ccagccagtg
    14101 atagattacc gccacgatat aacgttgttg ttcttcgctt agcgaaacac cctgcggaat
    14161 gttatgccac ttagccagac aaccgcgaca gcaggtcgcg gtggcgtgct gagcgataaa
    14221 caccggatgc ccgcgcatgg gggtttgctt accgtcatta gccggtaatg ccggagcaag
    14281 acgtttagcg acaaaatccg ccgcatgttg ttcgatgact ggcgcgcctt tctccagaca
    14341 atactgacgc tctttcatgc ccaaacgaaa gcgagatcga aattttgaac gggataaacg
    14401 cgcgaagagt ggatcgagag actgcattaa taaaccgaac gccctaactg tttcgtcaat
    14461 tgttcaagga cggcgatacc cgcaagcgag ttacctgcat catccagttc cggactccag
    14521 actgcgatgg ccatttcatg cggaacaatc gccacaatac cgccaccaac gccagatttc
    14581 gccggtagtc ccacccgcca ggcaaactcc cccgcgttct ggtacatacc actggtcgcc
    14641 atcagcgcgt taatttgccg tgcctgcatt ggcgtcacca caggttcatc aatatgaata
    14701 gctttcccct gattagccag aaagacaaac gtccgggcca gctctacaca gctcattttc
    14761 agagcgcagt aatgaaagta gttttgcaga acggttgtca cgtcatgatg gaaattgcca
    14821 aacgacttca tcagccaggc gatagccgca tttcgcgcgg aatgttcaaa ttcggaacgc
    14881 gctaccaccg tatcgtagga aatatcagac acaccgctta agccgcgcac gacttccagc
    14941 atacgttgcc gtggtgcgct taatcgccct tgcaacatat cgcagaccac cagcgcaccg
    15001 gcattaataa acggattacg cggaataccc tgctccattt ccagttgcac taaagaattg
    15061 aacggtgatc cagacggatc tttgccgacg cgttgccaga tttcctcttc ggagtaatga
    15121 cgcatggcga caacgagact cagcactttg gaaatagact gaatggaaaa acgttcttgc
    15181 gcgtctccgg cctgaaaaag ctgtccgtca acggtacaga tagcaatccc caatcgggaa
    15241 ccgtctactg tagccagcgc cggaatataa tccgcgactt taccctgacc aatgagcggc
    15301 cgcacttgcc gcaagatgtt ttctaaaatt gcattatcca tggcgactgc cactttctac
    15361 tcctggaccg caggtctgaa aagacctgcg agtatatcag agctgaatat gtcgcgtcag
    15421 atccggtctt tccacaccgt ctggatatta cagaattcgt gtaagccgaa atgggaaagc
    15481 tcacgaccaa agccactctt tttcacgcca ccaaaggcca ctcgcgcatc gctggcacaa
    15541 taaccattga taaacacccc accgcattcc agacgtgccg ccatctgtct ggcctgtgtt
    15601 tcgtcagtgg taaaaatggt cgctgaaagg ccgaactcac tatcattagc cagttccagt
    15661 gcatgttctg catctttcgc aatggtgatt gccgcaacgg ggccaaacat ttcttcccga
    15721 aacgcggtca tttctggggt aacattcgcc agaaccgttg gcggatagta gttacctgcc
    15781 ccagccatct tttccccgcc cagtaacaaa cgcgcaccct gcgccagggt tttctccacc
    15841 tgatgatgca gctcatcacg taaatcgaaa cgagccattg gtccgagagc gttctcttcg
    15901 tcacggggat cgcccatttt caaggctgcc gcagctgcca caaaacgttc ggtaaatgcc
    15961 gcagcaattc cctcttcgat aataaagcgt tttgccgctg cacaaacctg tccggtattc
    16021 tgataacgtc cggctaccgc cgctttaacc gccagttcca gatcggcatc gttaagcaca
    16081 ataaatggat ccgaaccgcc cagttccagt acgcattttt tcagtgccgc tccagcctgt
    16141 gcaccaatag ccgctccagc acgaacactt ccggtcaccg tgatagcagc aatgcgcgag
    16201 tctttaatca tctgagtgac accgtcattg tcggcattca gccagccata tacgccttgt
    16261 gggattcccg catctttaaa cacctgggca atgagctgag cacagcccat cacattcggc
    16321 gcatgtttaa gtaagtaacc gttaccagca agaatgatgg gaacagcgcc acgcatcacc
    16381 tgccataacg gaaaattcca cggcataatc gccagaatcg tccccaacgg tcgatactca
    16441 ataactgcct gctgattttc caccagcgta ggttccgcct tcagcattgc cggaccatgt
    16501 tctgcatacc agtcacacaa attcgccgat ttcgccactt cagcgcgcgc ctggttgatt
    16561 ggtttgccca tttcgcgggt gatcatttgc gccatttctt cgctacgggc gcgcagagcc
    16621 ttaccgatat cacgcagttt ttcagcacga taatctatat ttgtctcgcg ccagtcacga
    16681 aagcctgctg ccgccagctg aagtgcgttt tcgatatcat cagcgccagc ccacggcaac
    16741 acagaaagtt gttcacccgt ggcaggattt atcgaaattg catgagttgc cggagtaatg
    16801 gtcatcgggg tatctccttt atgagtcatg gtatgaagat acgcagattt actcttgctt
    16861 taaaatgaat aatattaagc cacttattca cgaatcgaga atattatgga tctgacccaa
    16921 ctggagatgt tcaacgcggt tgccgaagct ggcagcatta cgcaggctgc agcaatagtg
    16981 catcgcgtgc cgtcgaatct cactacccgt ctacgccagc tggaaacaga actgggggta
    17041 gatctgttta ttcgcgagaa tcagcgttta cgtctatctc ctgccgggca taacttttta
    17101 cgctatagcc aacaaattct cgcgttagtg gatgaagcgc ggagcgttgt cgctggcgat
    17161 gagccgcaag gtttattttc tctgggttcg ctggaaagca ccgcagcagt tcgcattccg
    17221 gccacgctgg cggaattcaa ccgtcgttat cccaaaattc agttttcact ttccaccggc
    17281 ccttccggca ctatgctgga aggtgtgctg gagggaaaac ttaatgcggc gtttattgat
    17341 ggacccatta accatactgc cattgacggg ataccggtat accgcgagga gctgatgatc
    17401 gtcacgccac aaggtcatgc gccagtaatc cgtgccagtc aggttaatgg cagtaacatt
    17461 tatgccttcc gcgccaattg ttcgtatcgt cgccacttcg agagctggtt tcatgctgac
    17521 ggtgccgctc cgggaactat ccatgagatg gagtcttatc acggaatgtt ggcctgtgtg
    17581 gtcgcaggag caggtattgc gcttattccg cgctctatgc tggaaagtat gccggggcat
    17641 caccaggttg aagcgtggcc gttagctgag caatggcgtt ggctaacaac ctggctggtc
    17701 tggcgtcgtg gtgcgaaaac gcgtccgctc gaggcattta ttcaactact ggatgtgcct
    17761 gactcgacaa aacagggtta ccaatgagct atttttgata gttcttgcgg ctaatatgct
    17821 ctatatagtg atgttccgat gacttatgac tatatggggc aaatatggtt acgccagtaa
    17881 gcatcagcaa ttacatatct cttcctgatg attttcccgt tcgtaatatt gcaccgcagg
    17941 taaaagaagt tttaaaggat tttattgatg cacttagtac aataatatgt aatgaagagt
    18001 ggcgcacgtc tttaaacatc aactcagcca caaaaaagat atttaataac cttgacaact
    18061 tatcatatat tcagaggact tcttttcggg gtaacgacac gctatacaat gaaaaggttc
    18121 agtttaaact cacttatccc gccaggaatg gaagacacaa agaaaatatt gaatttcagg
    18181 tagtaataaa tttaagccct atttatttag ataatttccg ccatgatgga gaaattaata
    18241 ttttttgcgc tcccaaccca aagcctgtca ctatggggcg cgtatttcag accggcgtcg
    18301 agcgtgtgct ctttctgttt ctgaatgatt ttattgaaca atttccaatg ataaaccctg
    18361 gcgttcccat caaaagagcg catacaccac atattgaacc cctgcctccg gatcaccata
    18421 ccgctgcaga ttacttacgc cagtttgatt tgcttgtcct gaattttatc tctcgcggta
    18481 attttgttat actcccccga ttatggaata actctgaggt tcacagatgg ttcgtcaata
    18541 aagatcctaa cttgatcacc gccattctcg acataacgga cagcgaatta aaagaggatt
    18601 tattgcaaag cctgatggat tcattaggtt ctaacaaaca tgtactaccc gaagtctgca
    18661 tctgcttttt atccctttta gcagaacaag aatctcctca ttttcaggac ttgtttttat
    18721 ttttcgccag tatgttattg cactatcacc aatttatgaa tcccaatgaa agtgatttga
    18781 ataacgtgtt gatgccagca tcattaagtg atgataaaat tatcaaacat atggcgcgca
    18841 agaccctcaa actttttgta aaaaatgaaa caccgccaaa agttactcat gaagacctgg
    18901 tgaaaaacag gcctcgctcc cctgtcagac cacctatacc cgcaacctcc aaaacgccag
    18961 acattcctga tcgtcattaa accgtgatgt ttaccgtctc tctgacgcgt gaaagaatca
    19021 gcgtcagaga aacggaaaac gcgatccaga tcacaaatgc attgtattca catcattaac
    19081 cgttttaaga tcatttcatc actttttcgc aactcacccg ataatctgtt atgacaacaa
    19141 acactgtttc ccgcaaagtg gcgtggctac gggtcgttac gctggcagtc gccgccttca
    19201 tcttcaacac caccgaattt gtccctgttg gcctactctc tgacattgcg caaagttttc
    19261 acatgcaaac cgctcaggtc ggcattatgt tgaccattta cgcatgggta gtagcgctaa
    19321 tgtcattgcc ttttatgcta atgaccagcc aggttgaacg gcgcaaatta ctgatctgcc
    19381 tgtttgtggt gtttattgcc agccacgtac tgtcgttttt atcgtggagc tttaccgttc
    19441 tggtgatcag tcgcattggt gtggcttttg cacatgcgat tttctggtca attacggcgt
    19501 ccctggcgat ccgtatggct ccggccggga agcgagcaca ggcattgagt ttaattgcca
    19561 caggtacagc actggcaatg gtattaggat tacctctcgg gcgcattgtg gggcagtatt
    19621 tcggttggcg aatgaccttc ttcgcgattg gtatcggggc gcttgttacc cttttgtgcc
    19681 tgattaagtt acttccctta ctgcccagtg agcattccgg ttcattgaaa agcctcccgc
    19741 tattgttccg ccgcccggca ttgatgagca tttatttgtt aactgtggtg gttgtcaccg
    19801 cccattacac ggcatacagc tatattgagc cttttgtgca aaacattgcg ggattcagcg
    19861 ccaactttgc cacggcatta ctgttattac tcggtggtgc gggcattatt ggcagcgtga
    19921 ttttcgggaa actgggtaat cagtatgcgt ctgcgttggt gagtacggcg attgcgctgt
    19981 tgctggtgtg tctggcactg ctgctacctg cggcgaacag tgaaatacac ctcggggtgc
    20041 tgagtatttt ctgggggatc gcgatgatga tcatcgggct tggtatgcag gttaaagtgc
    20101 tggcgctggc accagatgcc accgacgtcg cgatggcgct attctccggg atatttaata
    20161 ttggaatcgg ggcgggtgcg ttggtaggta atcaggtgag tctgcactgg tcaatgtcga
    20221 tgattggtta tgtgggcgcg gtgcctgctt ttgccgcgtt aatatggtca atcattatat
    20281 ttcgccgctg gccagtgaca ctcgaagaac agacgcaata gttgaaaggc ccattcgggc
    20341 cttttttaat ggtacgtttt aatgatttcc aggatgccgt taataataaa ctgcacaccc
    20401 atacatacca gcaggaaccc catcaggcgg gagatcgctt caatgccacc cttgcccacc
    20461 agccgcataa ttgcgccgga gctgcgtagg cttccccaca aaataaccgc caccaggaaa
    20521 aagatcagcg gcggcgcaac catcagtacc caatcagcga aggttgaact ctgacgcact
    20581 gtggacgccg agctaataat catcgcaatg gttcccggac cggcggtact tggcattgcc
    20641 agtggtacaa aagcaatatt ggcgctgggt tcatcttcca gctcttccga cttgcttttc
    20701 gcttccggtg aatcaatcgc tttctgttgc ggaaagagca tccgaaaacc gataaacgcg
    20761 acgattaagc cgcctgcaat tcgcagaccg ggaatcgaaa tgccaaatgt atccatcacc
    20821 agttgcccgg cgtaatacgc caccatcatg atggcaaaga cgtacaccga ggccatcaac
    20881 gactgacgat tacgttcggc actgttcatg ttgcctgcca ggccaagaaa taacgcgaca
    20941 gttgttaatg ggttagctaa cggcagtaac accaccagcc ccaggccaat tgctttaaac
    21001 aaatctaaca ttggtggttg ttatcctgtg tatctgggtt atcagcgaaa agtataaggg
    21061 gtaaacaagg ataaagtgtc actctttagc taaccttgca tcgcattggc caaaacttga
    21121 accgatttag caaaacgtgg catcggtcaa ttcattcatt tgacttatac ttgcctgggc
    21181 aatattatcc tctgcaacta attacttgcc agggcaacta atgtgaaaag taccagcgat
    21241 ctgttcaatg aaattattcc attgggtcgc ttaatccata tggttaatca gaagaaagat
    21301 cgtctgctta atgagtatct gtctccgctg gatattactg cggcacagtt taaggtgctc
    21361 tgctctatcc gctgcgcggc gtgtattact ccggttgaac tgaaaaaagt gttgtcggtc
    21421 gacctgggag cactgacccg tatgctggat cgcctggtct gtaaaggctg ggtggaaagg
    21481 ttgccgaacc cgaatgataa gcgcggcgta ctggtaaaac ttaccaccag cggcgcggca
    21541 atatgtgaac aatgccatca attagttggc caggacctgc accaagaatt aacaaaaaac
    21601 ctgacggcgg acgaagtggc aacacttgag catttgctta agaaagtcct gccgtaaaca
    21661 aaaaaagagg tatgacgatg tccagacgca atactgacgc tattaccatt catagcattt
    21721 tggactggat cgaggacaac ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt
    21781 cgggttactc caaatggcac ctgcaacgga tgtttaaaaa agaaaccggt cattcattag
    21841 gccaatacat ccgcagccgt aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg
    21901 agccgatact ctatctggca gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa
    21961 ccttcaaaaa ttactttgat gtcccgccgc ataaataccg gatgaccaat atgcagggcg
    22021 aatcgcgctt tttacatcca ttaaaccatt acaacagcta gttgaaaacg tgacaacgtc
    22081 actgaggcaa ttatgaaacc gcttttatcc gcaatagcag ctgcgcttat tctcttttcc
    22141 gcgcagggcg ttgcggaaca aacccagcaa ccgctcgtta cttcctgtgg cgatgtggtg
    22201 gttgttcccc catcgcagga acaaccaccg ttcgatttaa atcacatggg tacaggcagt
    22261 gacaaatcgg atgcgctggg cgtgccctat tataaccaac aagccatgta gtatattcag
    22321 gccccggcat atcggggctt gttaacctct tacctttacc gctttacgcc accgcaagcc
    22381 aaatacattg atatacagcc cggtcataat gagcaccgcg ccgaaaaatt gcagacccgt
    22441 taagcgttca tccaacaata gtgccgcact tgccagtccg actacaggca ccagtaacga
    22501 taacggtgca acccgccagg tttcatagcg tccaagtagt gctccccaga tcccataacc
    22561 aacaattgtc gccacaaaag ccagatacat cagagacaag atagtggtca tatcgatagt
    22621 gaccagactg tgaatcatgg ttgcggaacc atcgagaatc agcgaggcaa caaagaaggg
    22681 aatgattggg attaatgcgc tccagattac cagcgacatc accgccggac gcgttgagtg
    22741 cgacatgatc tttttattga agatgttgcc acacgcccaa ctaaatgctg ccgccagggt
    22801 caacataaag ccgagcatcg ccacatgctg accgttcaga ctatcttcga ttaacaccag
    22861 tacgccaaaa atcgctaagg cgatccccgc caattgtttg ccatgcagtc gctcaccgaa
    22921 agtaaacgcg ccaagcacga tagtaaaaaa cgcctgagcc tgtaacacca gcgaagccag
    22981 tccagcaggc ataccaaagt taatggcgca gaagagaaag gcaaactgtg caaaactgat
    23041 ggttaatcca taccccagca gcaaattcag tggtactttc ggtcgtgcga caaaaaagat
    23101 ggccggaaaa gcgaccagca taaagcgcaa accggccagc atcagcggtg gcatgttatg
    23161 aagccccact ttgatgacca caaaatttag cccccatacg accactacca gtagcgccaa
    23221 caccccatct tttcgcgaca ttctaccgcc tctgaatttc atcttttgta agcaatcaac
    23281 ttagctgaat ttacttttct ttaacagttg attcgttagt cgccggttac gacggcatta
    23341 atgcgcaaat aagtcgctat acttcggatt tttgccatgc tatttcttta catctctaaa
    23401 acaaaacata acgaaacgca ctgccggaca gacaaatgaa cttatcccta cgacgctcta
    23461 ccagcgccct tcttgcctcg tcgttgttat taaccatcgg acgcggcgct acgctgccat
    23521 ttatgaccat ttacttaagt cgtcagtaca gcctgagtgt cgatctcatt ggttatgcga
    23581 tgacaattgc gctcactatt ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata
    23641 agttcgacaa gaaacgctat atgttactgg caattacggc cttcgccagc ggttttattg
    23701 ctattcccct ggtggacagc gtcacgctgg tggtactttt tttcgcactc atcaactgcg
    23761 cctattccgt ttttgccaca gtgttgaaag cctggtttgc cgacaatctt tcatccacca
    23821 gcaaaacgaa gatcttctcg atcaactaca ccatgctcaa tattggctgg accgtcggcc
    23881 cgccactcgg cacgctgttg gtcatgcaga gcatcaatct gcccttttgg ctggccgcta
    23941 tctgctctgc gtttcccatg tttttcattc aaatttgggt aaagcgcagc gagaaaatca
    24001 tcgccacgga aacaggcagt gtctggtcgc cgaaagtttt attacaagat aaggcactgt
    24061 tgtggtttac ctgctctggc tttctggctt cttttgttag tggcgcattt gcttcatgca
    24121 tttcacaata tgtgatggtg attgctgatg gtgattttgc cgaaaaggtg gtcgcggttg
    24181 ttcttccggt gaatgccgcc atggtagtta cgttgcaata ttccgtgggc cgccgactta
    24241 acccggctaa catccgcgcg ctgatgacag caggcaccct ctgtttcgtc atcggtctgg
    24301 tcggttttat tttttccgga aacagcctgc tattgtgggg tatgtcagct gcggtattta
    24361 ctgtcggtga aataatttat gcgccgggcg agtatatgtt gattgaccat attgcgccgc
    24421 caggaatgaa agccagctat ttttccgccc agtctttagg ctggcttggt gccgcaatta
    24481 acccattagt gagtggccta gtgctaacca acctgccgcc ttcctcgctg tttatcatct
    24541 tagcgttggt gattattgct gcgtgggtac tgatgttaaa agggattcgt gcaagaccgt
    24601 ggaggcagcc cgcgctttgt tgatttaagt cgaacacaat aaaaatttaa ttcagccttc
    24661 gtttaggtta cctctgctaa tatgtttctc attgagatga aaattaaggt aagcgaggaa
    24721 acacaccaca ccataaacgg aggcaaataa tgctgggtaa tatgaatgtt tttatggccg
    24781 tactgggaat aattttattt tctggttttc tggccgcgta tttcagccac aaatgggatg
    24841 actaatgaac ggagataatc cctcacctaa ccggcccctt gttacagttg tgtacaaggg
    24901 gcctgatttt tatgacggcg aaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat
    24961 ggatagattt gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg
    25021 ttgacacagt agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc
    25081 acattttgtt cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt
    25141 gcccggtctg ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactgaca
    25201 cctgctgtca cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact
    25261 aactggcaaa ttctgacacc tgcacgacat gcttcttcat cattagccgc tttgacaata
    25321 atgataaatt cttcgccccc gtagcgataa accgtttcgt aatcacgcgt ccaactggct
    25381 aagtaagttg ccagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta
    25441 accaatttaa atcggtcaat atccaacaac attaaataaa gattcagagg ctcagtgttg
    25501 cgtaactgat gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca
    25561 tccatattgc tacggattgt cagcaaataa attttgtaat cggttaatgc cgcagtaaaa
    25621 gaaagtaacc cttcctgaaa agcggcgaaa tgcgcgtcct gccagtgatt ttcaacaata
    25681 gccagcatta attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga
    25741 acgtaaggta attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg
    25801 cacagtccat aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg
    25861 ctgtgaaaca tactcaccag ccctttttca cagttccttg tgcgcgctat tctaacgaga
    25921 gaaaagcaaa attacgtcaa tattttcata gaaatccgaa gttatgagtc atatctgaga
    25981 taacattgtg atttaaaaca aaatcagcgg ataaaaaagt gtttaattct gtaaattacc
    26041 tctgcattat cgtaaataaa aggatgacaa aaagcataac ccaaacccta atggcccagt
    26101 agttcaggcc atcgggctaa tttattttta tttctgcaaa tgagtgaccc gaacgacggc
    26161 cggcgcgctt ttcttatcca gactgccgct aatgttgatc atctggtccg gctgaacttc
    26221 tcgtccatca aaaacggccg caggaataac aacattaatt tcaccgctct tatcgcgaaa
    26281 aacgtaacgg tcctctcctt tgtgagaaat caaattaccg cgtagtgaaa ccgaagcgcc
    26341 atcgtgcatg gtttttgcga aatcaacggt catttttttt gcatcatcgg ttccgcgata
    26401 gccatcttct attgcatgag gcggcggtgg cgctgcatcc tgttttaaac cgccctggtc
    26461 atctgccaac gcataaggca tgacaagaaa acttgctaat acaatggcct gaaatttcat
    26521 actaactcct taattgcgtt tggtttgact tattaagtct ggttgctatt tttataattg
    26581 ccaaataaga atactgccaa ttgttataag gcatttaaaa tcagccaact agctgtcaaa
    26641 tattcagaga atttaactca ctaaagttaa gaagattgaa gagtcttaaa catattttca
    26701 gaataatcgt atttatatgt ttgaaaatta ttatattgga cgagcataca gaaaaagcaa
    26761 atcaccttta catataatag cgtggacaaa aaacagtgaa cattaataaa gataaaattg
    26821 tacaactcgc agataccgac actattgaaa acctgacatc cgcgttgagt caaagactta
    26881 tcgcggatca attacgctta actaccgccg aatcatgcac cggcggtaag ttggctagcg
    26941 ccctgtgtgc agctgaagat acacccaaat tttacggtgc aggctttgtt actttcaccg
    27001 atcaggcaaa gatgaaaatc ctcagcgtaa gccagcaatc tcttgaacga tattctgcgg
    27061 tgagtgagaa agtggcagca gaaatggcaa ccggtgccat agagcgtgca gatgctgatg
    27121 tcagtattgc cattaccggc tacggcggac cggagggcgg tgaagatggt acgccagcgg
    27181 gcaccgtctg gttcgcgtgg catattaaag gccagaacta cactgcggtt atgcattttg
    27241 ctggcgactg cgaaacggta ttagctttag cggtgagatt tgccctcgcc cagctgctgc
    27301 aattactgct ataaccaggc tggcctggcg atatctccag gacagccatt ggtggtgttt
    27361 atatgttcaa gccacgatgt tgcagcatcg gcataatctg aggagcctta ccgcgccatt
    27421 gtcgatacag gcgttccaga tcttcgctgt tacctctgga aaggatcgcc tcgcgaaaac
    27481 gcagcccatt ttcacgcgtt aatccgccct gctcaacaaa ccactgataa ccatcatcgg
    27541 ccaacatttg cgtccacaga taagcgtaat aacctgcggc atatccgcca ccaaaaatat
    27601 gggcgaaata actgctgcga tagcgtggcg gtatagcagg aagatccata ttttccgcca
    27661 ccagcgcccg caattcgaaa tcatcgacat cctgcattgc ttcgttttct tccaggcaat
    27721 gccagcgcat atcgagaagt gcggcgctaa gcagttcgct catctcatac cctttgttga
    27781 acaggctggc attacgcatt ttctgttgca gttcgtcagg cattgctgcc ccgctctgat
    27841 aatgccgggc gtagcgagcg aatacctgcg gatgcgttgc ccagtgttcg ttgatttgcg
    27901 acggaaattc gacaaaatca cgcggcgtgt tggtaccgga gagcgtggca taacgctggc
    27961 gggcaaagag gccgtgtagc gtatgaccaa attcatggaa taaggttatg acttcatccc
    28021 agagtaacaa cgcaggctca ccggcagcgg gtttctgata attgcagacg ttataaatta
    28081 ccggatgcgt ttcattaagc gttgattgct caacaaaatt gcccatccat gcaccgccgc
    28141 tttttgaatc acgggcgaag aaatcaccgt aaaataacgc cagccccacg ccattatgat
    28201 caaaaatttc ccacacccga acgtcaggat ggtagacagg aatatcaaaa cgttcgacaa
    28261 acttaatacc gaagagctga ttcgcggtcc agaatacacc ttcattcaac accgtgttta
    28321 attcaaaata tggcttgagc tgcgcctcat caagatcaaa tttctcccgc cggacctgtt
    28381 cggcataaaa tccccagtcc cacggctgcg cgctaaaccc gccctgctgc ttatcgataa
    28441 ccgcctgtat ggaggctaat tcatcgctcg cacgttgacg cgccgctgga acaatttccc
    28501 gcataaagtt aagtgctgct tcaggtgttt ttgccatctg atcggcgatt ttccatgcgg
    28561 cataatgagg aaaaccgagt aatgtagcct gttgtgcacg gatctccacc agacgttgaa
    28621 tgatagcgcg ggtatcattg gcatcatttt tttccgctcg cgtccagccc gcaataaaca
    28681 gtttttcacg cgtcgcacga tcgcgcattt cggcaagcgc cggttgttgg gtggtattca
    28741 gcagcggaat cagccatttg ttatccagac ctttctcgcg agccgcctct gccgccagcg
    28801 caatctcttg ctcactcatt cctgccagct gcgcgatatc gttcacaacc agactgccgg
    28861 atttatttgc tgccagtaac cgctggttaa actgactggt cagtgtcgca gcttctgtat
    28921 tcagtacttt taattttgct ttatcagctt gtgcaagttt ggctccggct aggacaaaac
    28981 gttgatgaat cacctccacc aggcggatgg attcactatc aagccccaga gattcacggc
    29041 gttgccagac agcatctacc cgcgcgaata attcaccgtt cagatagata tcattagcca
    29101 gttccgccag ttcagcggaa aactgctcgt caagacgctg taattcatca ttggtatgcg
    29161 ccgcagtcat ggcaaaaaag acgctggtaa cgcgggtaag taattctccg ctttgttcca
    29221 gtgccagaat agtattgttg aaatcaggtg tttgcgggtt aagcgcgatg gcagcaattt
    29281 ctgcccgctt ttgcagcatt ccctcatcga atgccgggcg atagtgatga ttggcaattt
    29341 gatcaaaatg gggagccaga tacggcagtg tgctttgcac aaggaaagga ttcattgttg
    29401 tcattttctt ctcctgaacg cgaggtgttc catagcgtag gcttactgat aacggagtgc
    29461 aatcttgcaa accagtatta cccgctctta agcatcccgt gctatgttat tgacacacaa
    29521 aagcgttgag gaacagtgag atgatcgttt tagtaactgg agcaacggca ggttttggtg
    29581 aatgcattac tcgtcgtttt attcaacaag ggcataaagt tatcgccact ggccgtcgcc
    29641 aggaacggtt gcaggagtta aaagacgaac tgggagataa tctgtatatc gcccaactgg
    29701 acgttcgcaa ccgtgccgct attgaagaga tgctggcatc gctccctgcc gagtggagca
    29761 atattgatat cctggtaaat aatgccggcc tggcgttggg catggagcct gcgcataaag
    29821 ccagcgttga agactgggaa acgatgattg ataccaacaa taaaggcctg gtatatatga
    29881 cgcgcgccgt cttaccgggt atggttgaac gtaatcatgg tcatattatt aacattggct
    29941 caacggcagg tagctggccg tatgccggtg gtaacgttta cggtgcgacg aaagcgtttg
    30001 ttcgtcagtt tagcctgaat ctgcgtacgg atctgcatgg tacggcggtg cgcgtcaccg
    30061 acatcgaacc gggtctggtg ggtggcaccg agttttccaa tgtccgcttt aaaggcgatg
    30121 acggtaaagc ggaaaaaacc tatcaaaata ccgttgcatt gacgccagag gatgtcagcg
    30181 aagccgtctg gtgggtgtca acgctgcctg ctcacgtcaa tatcaatacc ctggaaatga
    30241 tgccggttac ccaaagctat gccggactga atgtccaccg tcagtaattt ttatacccgg
    30301 cgtaactgcc gggttattgc ttgtcacaaa aaagtggtag actcatgcag ttaactcact
    30361 cacaagcaag aacgaatgac cgtcgaaacg caacttaatc ccacacagcc tgtcaatcag
    30421 cagatttatc gtatccttcg tcgcgacatt gtccattgcc tgattgcccc aggcacaccg
    30481 ttgtcggaaa aagaagtttc tgttcgtttc aatgtgtcac gccagccggt tcgtgaagcc
    30541 tttattaaac tggcggaaaa cggcctgatt caaattcgtc cgcagcgtgg cagctacgtc
    30601 aacaaaattt ccatggccca ggtgcgcaac ggcagtttta tccgtcaggc cattgagtgc
    30661 gcggtggcgc gtcgggcggc gagcatgatt accgaaagcc agtgctacca actggaacaa
    30721 aatcttcacc agcaacgcat tgccattgag cgcaagcaac tggatgattt ttttgaactt
    30781 gatgacaact tccatcaact cctgacgcag attgccgact gtcaactggc gtgggatacc
    30841 attgagaacc tgaaagcgac cgttgatcgc gtgcgctata tgagtttcga ccacgtttct
    30901 ccaccagaaa tgctgttacg ccagcatctt gatattttct ctgccctgca aaaacgtgat
    30961 ggcgatgcgg tagaacgtgc aatgacgcaa catttgcagg aaatcagcga atccgtgcgc
    31021 caaatccgcc aggaaaacag cgactggttt agcgaagagt aattcatttc ctctcatccc
    31081 atccggggtg agagtctttt cccccgcctt atggctcatg catgcatcaa aaaagatgtg
    31141 agcttgatca aaaacaaaaa atatttcact cgacaggagt atttatattg cgcccgttac
    31201 gtgggcttcg actgtaaatc agaaaggaga aaacacctat gacgacctac gatcgtaacc
    31261 gtaacgcaat caccactggc agccgtgtta tggttagcgg caccggtcac actggcaaga
    31321 tcctgtcgat tgatactgaa ggtctgaccg ctgagcaaat ccgccgcgga aaaaccgtag
    31381 ttgttgaagg ttgtgaagag aaactggcac cactggacct gattcgtctc ggcatgaact
    31441 aagcgtgtga atgccgccga tggcggcatt gcttttttac ttcacggaat atttcgccac
    31501 ggtcgctttc gcaccatgcg ctaataaaaa caagtacgct tccgtcactt ttgcagtaaa
    31561 caagctattg tctggcaaat catcaccaaa gatcgcctta atcgccagca atgactggac
    31621 gcgcgctttc ccttcggcac tactttgtac agccttctga ataacaggta acagtgggtc
    31681 actgatttca atcggatttc cctgttcatc aacaccaccg acataacgca tccaacccgc
    31741 gacgcccagc gccagcagat cgaacttgct gtcatgcgcc agatgccagc gaacagaatc
    31801 caacatccgc tgtggcaatt tctggctgcc atccatcgca atctgccagg ttcgatggcg
    31861 taacgccggg ttgctatagc gtgcaattaa tcggttagcg taatcttgca aatcaacgcc
    31921 ctgcactttc agcgtcggcg cttgttcctg caacatcaag gcatacgccg cataacgata
    31981 atgttcatct tccatacagt cattaatgtg ctgatatccg gcaagataac ccaaatacgc
    32041 caggaatgaa tgactgccgt tgagcatgcg caacttcatc tcttcataag gcagcacatc
    32101 actaaccagt tcggctcccg ctttttccca ttccggacgt ccggcaacaa agttatcttc
    32161 gattacccac tggcggaaag gttcacaggc aacgccagca ggatcgcgca caccggtaag
    32221 ctgttcgatt ttcgctagcg tatcctctgt cactgcgggc acaatacggt ccaccattgt
    32281 tgatgggaaa gtcacgttat cttcgatcca ttgtgccagt tttacatcaa cggcttgtgc
    32341 gtatgaagtg acaacgtcac gcataacatg accgttttct ggcatgttgt cacatgacat
    32401 gacggtaaat gcgggaagtc ctgccgcttt acggcgagcc agcgcctcaa caatcacccc
    32461 tgttgctgtt ttcggctggt ggggattttg cacgtcggca gcgaccatcg ggtgatcgag
    32521 cattaactgt ccggtcgccg gagagtggaa ataccctttt tcggtgattg tcagagagac
    32581 aatcgcgatt tgcggttcac acatcgcagc caacacggtt tctaagccat ccatctgtac
    32641 gtgcaaggct tttttaacga cgccaacgac gcgtgccgtc cacgcatcgg ccgacatttc
    32701 cgcaacggta taaagattat cttgctgttg taaatcggca atttgctgtt cgccaccgat
    32761 taagttgacc tcataatatc cccagtcact gaaatgttct gtagcaagaa tatcggcata
    32821 cacgccctga tgcgcacggt gaaatgcacc aaagcctaaa tgaacaattc ttggggccaa
    32881 gttattacga tcataaacag ggagcgtcgc ttttgctgat aacaaattat ttcccataac
    32941 tattccttaa atataaatat ggcaagctac atgttttgtt atatgaataa aaaatcccct
    33001 ctccggtaag agaagggatt gaggatttaa agacttctgg aaggttgcgc agctcttaca
    33061 acacgcggtt gatcttccgc agcgtcttcc agcgcactta aatcacggtc tttcacttct
    33121 ggcattttca gcgcagagat taaaccaatc actgaatatg ccatgatcat aatggcgatc
    33181 ggataccagg attccgtcat ggtgcagaaa atacccgcaa ggataggacc aaaaccggaa
    33241 gcgataagac caccaatttc tttagaaata gccatccggg taaagcggtt tttacagccg
    33301 aacatttctg ccatggtaat gttttccaga gcaaataatc ccagcaccgc acagttatga
    33361 atcacaatca gtgcaaccat aatggtgctc ggggcatagc ttttatctac gatgatagaa
    33421 agcattggcc atgccagcac aatcgcagag gtattcataa taatatacgg gatccggcga
    33481 ccaattttat cggataacca accaaggaac ggaatggtca taaagccgag aatcgaactg
    33541 atcatcaatg catctgttgg aattgctttg ttaaacaata acgtctgtac taaatagcct
    33601 gcaaggaaag tctgaattaa cccggagtta cctgcctgac caaaacgcag ccctgttgcc
    33661 agccagaagg atttgctctg gaacatgcta ccagcaggtg caggttttgc tgtcggttgg
    33721 ttgctgtcgt taaccttctc aaagaccggg ctttctttca gattcatacg caaccagata
    33781 gcaaagacca tcacgacaac gctcgccagg aacggtatac gccatcccca cgccagcagt
    33841 tcctctttac tgagaatgaa gaacataaag gcccagattg ccgttgcgct caaggttccg
    33901 cagttagttc ccatagccac aaatgaggag ataattccgc gcttaccttt tggcgcatat
    33961 tccgccagca tcgtaccggc accggaaatt tccgcacctg cacccaatcc ctgaataata
    34021 cgcagcgtca ccagcaagat gggggcaaaa acaccaatct gtgcataggt cggtaacact
    34081 ccaattaagg tggtacagat ccccatcatg gtgatggtaa taaagagcac ttttttacgc
    34141 cctattctgt cgcccatttt gccgaaaata aatgctccga caatacgcgc cacataacct
    34201 gcaccgtagg ttcccattgc cagaattaac gccattgccg ttgatgattc aggaaaaaat
    34261 atttcatgaa acactaacgc tgcgccgagc gaatataact ggaaatccat aaattcaagc
    34321 gcagtgccca gccatcccga tactgctgct ttcaccaaat ccttagtgct tctttcgtgt
    34381 ttttctattg tcataatggt tatctctaaa aaagaggtaa gatgcgtact acttactcgc
    34441 cgttattggt attattcaga aaaagtgagt aagactttgc agcaatgttt ttgatcctgt
    34501 tcaaataaac taatggcatc agcaacatgc tggaaatcaa acgtatgggt aattaatttt
    34561 tctggtttaa ttaacccttt acttaaccag tcgataacga ccggaaattt atttgcattt
    34621 aagcgtgaag agaaaataga gagttctttt ccggtaattc cttgctgaat cacttcagaa
    34681 ggttcactgg agaagcccat caatacaata cgtgccgctg gagaagccag cgttacggcc
    34741 tctttcagga tagaaggatg acaagccgca tcgataatta atgtcggctt tatgcctttt
    34801 tcagcgaaaa tctcgccaag cggtgtctgg ctgttattaa tcacccaatc agccccgctc
    34861 tctttcgctt tttccagtcg ttcatcaatg cgatcggcaa caatcacatt tttaacgtta
    34921 tagacgcctt ttaatacctg aacgatcgtc aggccgattg gaccggcacc ataaaccaga
    34981 acggtatcat tttcagtcgg ttgaccatgt ccggttacgt tagccgcaat ggtaaaaggt
    35041 tcgatcatca ccgcatattg atcggccact gcttcaggaa ttttccacgc attttttgcc
    35101 ggaaccacag catattcact gaaaccaccg tcaacatgca cacctaatac agcgagtgtc
    35161 gtacaaacgt tcggcttacc tatagagcac ggatagcaat gaccacagct gaccaccgga
    35221 tcgacagcaa cacgttcacc gactctggcg ctttccacac cttcacccac cgcatcaatg
    35281 acgccaaaga attcatgtcc aatgacgcgc ggatatttcg caaaaggatt atgcccacga
    35341 taaatatggc tatctgaacc acaaattccg gcaagtttta cttttactcg aacttcaccc
    35401 gctgacgggg tgggtatttc acgttcgata atcgccagtt gattcggttt ttcaattaaa
    35461 atgcttttca ttaacttact ccttaccagt tccacagcgt gccatcttcc agacgtgcga
    35521 ctggtagata agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatgc
    35581 caagacccgg tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga
    35641 agacttcgag catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc
    35701 acagatcaaa gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg
    35761 agccagtacg tacctgatac aacgaagcaa aatcggcaat ccggcgcata ccggtaattc
    35821 cgcctgcatg ggtcagcgtg gtgcggatat aatcgataag ttgctcttca atcagttgct
    35881 tgcagtccca gatgctgttg aagacttcac ccaccgcgat gggtgtgatg gtatgttggc
    35941 gaatgagacg gaagcattcc tggttttccg caggcgtcgg gtcttccatc cagaacatgc
    36001 gataatcttc aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca
    36061 tgtcatgcag caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg
    36121 gcatgaaatc gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg
    36181 ttgcgggttc ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag
    36241 ggatgccgca ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat
    36301 ccagagcttc atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag
    36361 acgcgccgcc gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca
    36421 gcgccatatc aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac
    36481 ctttatagaa aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagctgcg
    36541 gacaaaggtg atcctgcaaa taagaggcca cagaaagctc acgtccattg agggtggcat
    36601 ccccaaggcc cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg
    36661 gacaggtaac aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt
    36721 cgtgatgcat gaaatctacg caactgagct actaccatac aagtatgaag atcaaaaaaa
    36781 gccggagtga tcacaaaaaa aggcgtatat tttcgccatg aatggttgac aaaagatgaa
    36841 atagagtacc ttttatcagc tgacgcttcc tcgtatctta ttgataaaat agatttatgt
    36901 ccttacgtgc gcccccagcc ggcaacaatg atcaacatgc cgcaaagcgc aatcaacgca
    36961 cccgtccagt cataaagagt cagtttcacg ccatccacaa cgcgcagcca catcaacgcc
    37021 gtgcagacat aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac
    37081 gttaacaacc agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg
    37141 gcgtttcgtt ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta
    37201 gcaaaaaata gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta
    37261 tagggcgaca taatatcatc aatataaaca ccctcctggt acgttttgcg ttcgcagtgg
    37321 atggtgtaga atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag
    37381 caaacgaatc ggcctgctcg cttttctgct gccttgcaca ctggcattga gcacaactgt
    37441 tcatgccgaa actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca
    37501 cgccgctatg gaaaaagagc aatggaatga cacgcgcaat ctgcgtcaga aagtgaataa
    37561 acgcactgaa aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga
    37621 gcaaagcgcc aacatcaatg cctactggga gccaaatact ttgcgctgcc tggaccgtcg
    37681 aactggccgc gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta
    37741 tgccaagcgc ccacagtgtt aagctacgcc cgctggagcg tgaagattta cgctatgtac
    37801 atcaactcga caataacgcc agtgtgatgc gttactggtt tgaggaaccc tacgaagcct
    37861 ttgttgaact ctctgatctg tatgataagc atattcacga tcagagcgaa cgacgctttg
    37921 tggtggaatg tgacggcgaa aaagccggtc tggtggagtt ggtggaaatt aaccatgtac
    37981 atcgccgcgc agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa
    38041 cccgtgccgc caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt
    38101 atctgatcgt tgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta
    38161 cggttgaagg tgaattgatg catgagttct ttattaatgg tcaatatcgt aatgccattc
    38221 gcatgtgtat attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc
    38281 tgaagccgac cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc
    38341 gttcgacggc gggttttact ccttcatcaa caatgatcag ctgacagtcc accggattgg
    38401 cgtgactgtc atattcacag ctctgtttta cattaccaac tcgttgatta ttcagtaaag
    38461 taaccgctgt gtaatctaat tttttgatcg ggtccgttga tggcgtggcg ctgacagata
    38521 atgttttgtc gttacttttc gtggttttac ccagtggata accctgatca tcatagcgat
    38581 attccatctg catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc
    38641 aactcacccc ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct
    38701 tctccagcgt ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat
    38761 tattttccag atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtccccg
    38821 aaacacgttt cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg
    38881 gaccacgcag cggatcaaaa tcgaattcat tagagaaact ggccatctca ggagtaaatg
    38941 aaagtgttac ctctgtgcgg tcacatcctg tgagtaatat cgcgagtaag catggcagta
    39001 atttgtattt cacatcagtc accagagagt agagatgatt ctcaatcata gtagcaaata
    39061 cagtacttta cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg
    39121 atgaaactct caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc
    39181 gccgcgccgg gatcctgtga gcgcatacaa agcgatatat cacagcgcat tatcaataat
    39241 ggcgtacccg aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct
    39301 gattcccagg tcgtcgggca ttgcgctaat gatacgcata aaattctcta cacccgcacc
    39361 accagcggta acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg
    39421 cagtaataca ttatcgactg aacgccggat acgacgcatc cggcgatttg aacatataac
    39481 ataacccacc ttattactca tacccttcta ttgatatgga ttaataattc ttaacccaaa
    39541 atgggtagac tccctctatt gttagcgcgc taaatattca atatataaac ttttatataa
    39601 cgataaagaa cagggagtga gttatgtcca aaaatgaaca aatggtgggc atcagccgca
    39661 gaacactcgt taaatctacc gcgataggtt ctctggcgct ggctgcaggc ggtttttctt
    39721 tgccgttcac cctgcgcagt gcagcggcag cagtacaaca ggccagcgaa caagtggtct
    39781 ggggtgcctg ttccgtcaac tgtggtagcc gctgtgcact tcgtctacat gttaaagata
    39841 atgaagtggc ctgggtggaa actgacaaca ccggcagcga tgagtatggc aaccatcagg
    39901 tacgcgcctg tttgcgcggt cgctccatcc gccggcgtat taatcatccc gatcgcttga
    39961 attacccaat gaaacgcgtg ggtaaacgcg gtgaaggcaa attcgaacgg attagctggg
    40021 atgaagccct ggatactatc gccagtagcc tgaagaaaac cgtcgaacaa tatggcaatg
    40081 aggctgtata tattcagtac tcttcgggga tcgttggcgg caatatgacc cgctcttcgc
    40141 catcagcctc ggcggtcaaa cgcctgatga actgctacgg cggttcactc aatcagtatg
    40201 gctcctacag caccgcacaa atttcctgtg ccatgcccta cacctacggc agtaatgatg
    40261 gcaacagcac cacggatatt gaaaacagca agctggtcgt gatgtttggt aacaacccgg
    40321 cagaaacccg catgagcggc ggtggcatca cttatcttct tgaaaaagcg cgcgagaaat
    40381 cgaacgccaa aatgattgtt atcgatccgc gatataccga tacggctgct ggtcgtgagg
    40441 atgaatggct tcctattcgc ccgggcaccg atgccgcgct ggttgcgggg attgcctggg
    40501 tattgattaa cgaaaatctc gttgatcaac cttttctcga taaatactgc gtcggctatg
    40561 acgaaaaaac cttacccgca gatgcaccaa aaaatggtca ctataaagcc tatattcttg
    40621 gtgaaggtga tgataacaca gcgaaaacgc cgcagtgggc ttcgcaaatt accggtatcc
    40681 cggtggaccg tatcatcaaa ctggcgcgtg aaattggcac agcaaaaccc gcttatatct
    40741 gccagggctg ggggccgcaa cgccaggcaa acggcgagct gactgcacgc gctattgcta
    40801 tgttgcctat tttgacgggc aatgtcggca tcagcggcgg aaatagtggc gcgcgtgaat
    40861 cgacctatac cattaccata gagcgcctgc cggtgctgga taatccggtc aaaacgtcaa
    40921 tctcctgctt cagctggacg gatgctatcg atcatggccc gcaaatgacg gcaatccgcg
    40981 acggcgtccg cggcaaagat aaactggatg tgcccattaa gttcatctgg aactacgcgg
    41041 gaaataccct ggttaatcag cattctgaca tcaacaaaac gcatgaaatt ctgcaagacg
    41101 aatcgaaatg cgaaatgatt gtggtcatcg aaaactttat gacctcatcg gcaaaatatg
    41161 ccgacattct gctgccagac ctgatgaccg ttgagcagga agatattatt cctaacgact
    41221 acgccggtaa catgggatat ctcattttcc tccagcctgt caccagcgaa aaattcgaac
    41281 gcaaaccgat ttactggatc ctgagtgaag tcgcgaaacg tcttggacct gacgtctatc
    41341 aaaagttcac agaaggtcgc acgcaggaac aatggttaca acatctgtac gccaaaatgc
    41401 ttgccaaaga tccggcgtta ccgtcttacg acgaactgaa aaaaatgggt atctataagc
    41461 gtaaagatcc caatggtcat tttgtcgcct acaaagcatt tcgtgacgac cccgaggcaa
    41521 atccacttaa aacaccttcc ggtaagattg aaatttattc cagcaagctg gcggaaattg
    41581 cccgtacctg ggaactggaa aaagatgaag tgataagccc actgccagtt tatgcctcta
    41641 cctttgaagg ctgggactca cctgaacgta gaaccttccc actgcaactg tttggtttcc
    41701 attacaaatc ccgtactcac tcgacctacg gcaatattga tctcctgaag gctgcctgcc
    41761 gtcaggaggt gtggatcaac cctatagatg cgcagaaacg tgggattgcc aacggcgata
    41821 tggtgcgggt gtttaaccat cgtggcgaag ttcggctacc agccaaagtg acaccacgca
    41881 ttctccctgg ggttagcgct atgggccagg gagcctggca cgaggccaat atgtctggcg
    41941 ataaaatcga ccatggaggc tgtgtgaata cgctaaccac tctgcgccct tcaccactgg
    42001 cgaagggaaa cccgcagcat acgaatctgg tcgagatcga aaaaatataa cccactacaa
    42061 ccataaattc tggcatgaca ttttgtttga aaagcaataa gtgagtaatg atgaaaatcc
    42121 ataccacaga ggcgctcatg aaggctgaaa tcagccgtag aagtctgatg aaaacctccg
    42181 cacttggcag tcttgcgctg gcaagcagtg ctttcactct gccattttcc caaatggtcc
    42241 gggcggcaga ggctccggta gaagagaaag cggtctggag ttcctgcacc gttaactgcg
    42301 ggagccgctg tctgttacgt ttgcatgtga aagatgacac cgtgtactgg gtggagtctg
    42361 atacgacagg tgacgacgtc tacggtaatc atcaggttcg agcgtgttta cgcgggcgct
    42421 ctatccgccg acggatgaat catcctgata ggttgaaata tcccatgaag cgcgtcggca
    42481 agcgcggtga aggtaaattt gaacggataa gttgggacga agccctggat accatcagtg
    42541 ataatcttcg gcggatcctg aaagattacg gcaatgaggc tgtacatgtc ctgtacggaa
    42601 caggtgtaga tggcggaaac atcaccaact caaacgtccc gtaccgtctg atgaactctt
    42661 gcggtggctt tctcagtcgc tatggtagct acagtaccgc acagatcagt gccgcaatga
    42721 gttatatgtt cggtgccaat gatggcaaca gcccggatga tatcgccaat acgaaactgg
    42781 tcgttatgtt cggaaataac ccagcagaaa cgcggatgag cggcggtggt gtcacttact
    42841 acgtcgagca agcccgcgaa cgttcaaacg cacgcatgat cgtcatcgat ccacgttata
    42901 acgacactgc tgccgggcgt gaagatgaat ggctgcccat tcgccctggc accgatggcg
    42961 cactggcctg tgcgattgcc tgggtactga ttactgaaaa catggtcgat cagccatttc
    43021 tcgacaaata ttgtgttggt tacgatgaaa aaacgctgcc cgccaacgca ccacgtaacg
    43081 cgcattataa agcctatatt ctgggcgaag ggcctgacgg catagctaaa acgccggaat
    43141 gggcagcaaa aatcaccagc atcccggcag aaaaaattat ccagttggca cgagagatcg
    43201 gttcagcaaa acctgcttat atttgtcagg gttgggggcc acaacgacac tccaacggcg
    43261 aacaaacatc ccgcgctatt gccatgcttt ccgttctcac cggtaacgtc ggcataaacg
    43321 gcggcaactc aggcgtacgc gaaggtagct gggatctggg ggtagaatgg ttcccgatgc
    43381 tcgagaatcc tgttaaaacg cagatttccg tctttacatg gacagatgcc atcgaccatg
    43441 gtacggaaat gaccgcgacc cgtgatggtg ttcgtggaaa agaaaaactg gatgtcccca
    43501 tcaagttttt atggtgctac gccagtaaca cattgatcaa tcaacatggc gacatcaatc
    43561 acacccatga ggtgcttcag gatgacagca agtgcgagat gattgttggc attgaccact
    43621 tcatgacggc ctcggctaag tattgcgata tcctgttgcc cgacctgatg ccaacagagc
    43681 aagaagacct tatctctcat gaatctgcag ggaatatggg ctatgtgatc ctcgcccaac
    43741 ccgcaacctc agcaaaattt gaacgcaaac ccatctactg gatgctgagt gaagtcgcca
    43801 aacgcttagg accggacgtt tatcaaacct ttactgaagg tcgcagtcag catgaatgga
    43861 tcaaatatct ccatgcgaaa acgaaggaac gtaaccctaa gatgcccgac tacgaggaga
    43921 tgaaaacgac cgggatcttt aagaaaaaat gcccggaaga acactacgtc gctttccgcg
    43981 cattccgtga agatccacag gcaaacccgt tgaaaacacc ttcggggaaa atcgaaattt
    44041 attctgaacg actggcgaag attgcagata cctgggaatt gaaaaaagat gaaattattc
    44101 atccccttcc tgcgtatacc ccaggttttg atggctggga cgatcccctg cggaaaacct
    44161 atccactgca gttaacgggc ttccactaca aagcgcgtac ccactccagc tacggcaata
    44221 ttgatgtgtt acagcaggcc tgcccacaag aggtgtggat caaccccatt gatgctcagg
    44281 ctcgcggtat ccgtcatggc gataccgtgc gggtatttaa caataatgga gaaatgctga
    44341 ttgccgcaaa agtgactccg cgtattctgc ctggcgttac cgccatcggg caaggtgcgt
    44401 ggcttaaagc ggatatgttt ggtgaccggg tcgatcacgg cggcagtatt aatattctga
    44461 cctctcaccg cccttcaccg ctggcaaagg gaaacccgtc gcacagcaat cttgtccaga
    44521 tcgaaaaggt ttaaggagta gcccatgacc acacaatatg gattttttat cgattccagc
    44581 cgctgtactg gctgtaaaac ctgcgaactg gcgtgcaaag attttaaaga tcttggcccg
    44641 gaagtcagtt tccgccgtat ttatgaatat gctggcggcg actggcagga agataatggc
    44701 gtctggcacc agaacgtgtt cgcttattat ctctccatct cctgtaacca ttgcgatgac
    44761 cccgcatgta caaaagtctg ccctagcggc gcaatgcata agcgtgaaga tggctttgtg
    44821 gtggttgatg aagatgtttg tatcggctgc cgctactgcc acatggcttg tccgtacggc
    44881 gcgccacagt acaatgctga aaaagggcac atgacgaagt gcgatggttg ttattcgcgc
    44941 gtcgccgagg ggaaacaacc catatgtgtc gaatcctgcc cgctgcgtgc acttgagttc
    45001 ggtccaattg aagagctgcg ccagaagcac ggcactctgg ctgccgtcgc gccactgccg
    45061 cgcgcgcatt tcacaaaacc caatatcgtt atcaaaccta acgccaacag ccgcccgacc
    45121 ggcgatacca ctggttatct ggctaatccg gaggaagtgt aaaatgggaa atggatggca
    45181 tgaatggcca ctggttatct ttaccgtatt gggtcaatgc gtagtaggcg cgctgattgt
    45241 tagcggaatt ggctggttcg ccgccaaaaa tgacgcagac cgtcagcaca ttgttcgtgg
    45301 catgtttttc ctctggctgt tgatgggcat cgggtttatt gcctctgtca tgcacctggg
    45361 atcgccactg cgggcgttta actcgctgaa tcgaatcgga gcttctggtc taagtaacga
    45421 gattgctgcc ggttccatat tttttgccgt tggcggacta tggtggctgg tggcggtcat
    45481 cggtaaaatg ccgcaagcgt tggggaaact ctggttactt gtcagcatgg cgctcggcgt
    45541 cattttcgtc tggatgatga cctgcgtgta tcaaatcgac actgtgccaa cctggcataa
    45601 cggttacact acgctggctt tcttcctgac ggtattgttg agtggtccaa tacttgctgc
    45661 cgcaattctg cgggcagccc gcgttacatt taataccaca ccatttgcca tcattagcgt
    45721 tctggcgctg atcgcttgtg ccggggtgat tgtgctgcaa ggtttgtctc tgtcgtctat
    45781 tcactcatcc gtgcaacaag ccagcgcact ggtaccagat tacgcctcct tacaggtctg
    45841 gcgtgtggta ttgttatgcg caggtctagg atgctggctt tgtccgctca ttcgtcgccg
    45901 tgaacctcac gttgccggac ttattctggg gttaatcctc attcttggcg gtgagatgat
    45961 cgggcgtgtg ctcttttatg gcctgcacat gacggtagga atggctattg caggttaaca
    46021 aattggtgcg gggcaacccg cacatttcag gatgttagga atgacccatt tttcacagca
    46081 agataatttt tctgtcgtgg cgcgcgtgtt gggcgcgctg ttttattacg ctccagagag
    46141 tgcggaagcc gcacctctgg ttgcggtact cacccgtgac ggctgggaaa ctcagtggcc
    46201 tctaccagaa gcgtcattag cgccgctggt gacggctttt cagacacagt gtgaagagac
    46261 tcacgcccag gcctggcagc gtttgtttgt cggcccgtgg gcactgcctt caccgccgtg
    46321 ggggtcggtc tggctggatc gcgaatctgt gctgtttggc gattcaacat tggcacttcg
    46381 tcagtggatg cgcgagaaag gcattcagtt tgaaatgaag caaaacgaac cggaagatca
    46441 ttttggttca ctgctgttga tggctgcgtg gttggcggag aatggtcgcc agacagaatg
    46501 cgaagaattg ctggcatggc acctttttcc gtggtcaaca cgttttcttg acgtttttat
    46561 cgaaaaagca gaacaccctt tctaccgtgc actgggtaaa ctggctcgct taacgctggc
    46621 gcaatggcaa tcacaactgt taattcctgt cgcggttaaa ccgctgtttc gataggatca
    46681 ccataatatt gccggatggt gacgattacg cgcaattcgg caataatacg ttacagtgtc
    46741 acgttttttt ttatctctta aagcacgcac tgcttttgcg gctggcctct tttgccgcaa
    46801 aatagtcgcc cgtgtttcat tgcccatttc tgctcatgca tcatctacac atctatccga
    46861 atctgcgcac tatgttccgc cgtctgctta tcgcaacagt cgtcggtatt ctcgcggcct
    46921 ttgccgttgc cgggtttcgt catgcgatgc tgctactgga gtggttgttc ctcaataatg
    46981 actccggcag tctggtcaat gcagcgacaa acctttcccc ctggcgacgg ctgctaactc
    47041 cggcgctcgg cggactggcg gcgggtttgt tgctgatggg ctggcagaaa tttacccagc
    47101 aacgccctca tgcgccgacc gattatatgg aagcgttgca aaccgatggt cagtttgatt
    47161 acgcagcaag cctggttaaa tcgcttgcct ctctgctggt agtaaccagc ggcagtgcaa
    47221 ttggtcgcga aggtgcgatg attcttttag ctgcccttgc cgcctcctgt tttgcccaac
    47281 gttttacgcc acgccaggag tggaaattat ggatcgcctg tggggccgcg gcgggaatgg
    47341 ctgcggccta tcgtgccccg cttgctggca gtttatttat agccgaagtg ctgtttggca
    47401 ctatgatgtt ggcctctctc ggcccggtga ttatttccgc cgtcgtggca ttgctggtta
    47461 gcaatctgat taatcatagc gacgcgttac tctacagcgt acaactctca gtgacggttc
    47521 aggctcgtga ctatgcgctg attatcagta caggtgtgct ggcaggtctg tgcggaccac
    47581 tgttgttaac gttaatgaac gcctgtcatc gtggatttgt gagtctcaaa cttgcgccgc
    47641 cctggcaact ggcactaggc gggttgatcg tgggtctgct ttccctgttc acacctgcag
    47701 tgtggggcaa cggctatagc accgtacaat cctttttaac cgccccgcca ctgttaatga
    47761 tcattgccgg gatcttcctt tgtaaactgt gtgccgtgct ggcgagtagc ggttccggcg
    47821 cacccggtgg tgtctttaca ccgacgctat ttatcggtct tgccattggc atgttgtatg
    47881 gtcgtagcct gggattatgg ttccctgatg gcgaagaaat tacgctttta ctcggattga
    47941 ccgggatggc gacactgttg gcggcaacca cgcacgcgcc gattatgtcg acgttgatga
    48001 tatgtgaaat gaccggggag tatcagctac tccccggttt attgattgcc tgcgtaattg
    48061 cgtcggtaat ctcgcggacg ttacaccgtg actctatcta ccgccagcac actgcgcagc
    48121 atagctaagc ggatgtattg ccccagttca cgctgttcgg cgcgcggcag ataaggcagt
    48181 tcaccaatga gcggtgccgg aagtttttta ccgagcacat caatgatttc cgcataatgc
    48241 gccagtcctg ggttgattcg gttagccacc cagccaatga gcggcagccc gtcgttggca
    48301 atcgcctgag ctgttagcag tgcatggtta atgcaacctt cctgaatacc gacaaccatc
    48361 aacaccggca gttgttcctg caccacccat tcagagagtg gacgcaaatc attcatcaga
    48421 ctgcgccagc cgccagtccc ttctaccacg acatgatcga ctttatcggt caggtttgcc
    48481 aggccgtttg aaatgagggt gtaattgatt gggcaactgt gcgctacgct actttcttct
    48541 tcgcttaacg cgataggatt aactgcttca taaggcagtt cgatggttga aacactctgc
    48601 aacaccaggg catctttatt acgcagccct tcgggtgtct ctttgctccc cttcgctacg
    48661 ggtttatatc ccgcaaccgt ttttccctgg gaggctaacg cttgtagcaa tgcgcgggaa
    48721 accaccgttt tccctacaga agtgtctgta ccggtaataa agaaacgctt cagcatcact
    48781 aactccaccg ttatgcttca caaatataaa ccaggaaaat aattaacctt gaaagtctaa
    48841 gttatgcttt cctggcccaa attgagatag cgcaaatttt ggtagaacag ttaaaaaatg
    48901 ttaaccctgc aacagacgaa tcaacaaaga accgttatac atcgcgtctt ttaccagcgc
    48961 agcccctgcc atcgtgccct ggttagaaaa ctgagtactc tcaacgctaa tgtgctgact
    49021 atacgcagga agggcctgct gacggatgct atctgagatg accgggaaga ggatatctgc
    49081 cgctttactt aacggtgagc caatcagtat tttttgtggg ttaaataaat tcaccatgat
    49141 ggcaagaatg cgccctacat gcgcgcccac cccggtaatg atgtcttttg ccagcagatc
    49201 gccgcgcaat gccgcctgac acaatgagtc cacggttaac ggttgcccat gtaacatcga
    49261 gctcatggat tgattgagac gcagctgtgc cagctcaaga atactgtcca cactggcgat
    49321 ggtttcgagg cagccgtggt tcccgcaata acagcgttta ccatatgggt ctacctgtgt
    49381 gtgacctatt tccacgaggc tactgctgcc tgcgtgtagc agatgaccat cggtaatgac
    49441 gcccgccccc acgttgtgat cgataaccac ctgaatcaca tcgcgcgccc cgcgtgaggc
    49501 accaaacaag gcctctgcca tcgtccatgc gctgatatca tgctgaatat aaaccggaac
    49561 gccggtatgc tgctccagag cctctccgag cggcatctct tttacatcct cgtagaacgg
    49621 catgcgatgt acaatgccat tttccgtatc aataattccc ggcagggtta tggcaatcga
    49681 agttagacgc tcaagttttt tctggtggcg gataaaaaac tgatcgatat gggaaatgat
    49741 acgatccagc aatggcgagt catcttttaa cgccagttcc tgcgactctt ccaccaccag
    49801 tttgctgctc agatcgcgca gagcaaggaa aatctccccg cgactaatgc gcagagaaag
    49861 atagtgccag gcttcagttt caaccaccag ccccaccgcc ggacggccac ggttccccgc
    49921 ttctttgatt tccagctctt gcaccaggtg tgcttcgagc atctcacgga caattttagt
    49981 gatactggca ggagccagct gcgccagacg ggaaagatcg atacgcgaga ctggaccaag
    50041 ctgatcaatc aggcgataaa ccgcgcccgc gttggtctgc tttatttgat caatgtgccc
    50101 aggctggttt tcagcaacca ccgcatactc cctatatttt cgcgctccga aataatctgt
    50161 aggctatggt gaagcacttc aatacgtgtc gtcaaatttt tacttaggca tgtgattaac
    50221 agcacatttt tcgggctttt tcgctgaaat ttccctcacc tgagagcatt cagcagatga
    50281 atacgaaagt tacgcgcagc tggactttgt tcatgatgtt tcggccagac caaccacatt
    50341 tcagaaaccg catcctcttc agcaatcggc acccagcgca tttcgttgag ctgaacacgt
    50401 ttaaatgacg caggcaaaat tgaaacaccc agaccggcgg aaaccagacc gatgatggtc
    50461 attgcctcgc ccacctcctg agtgatgacg ggcgtcaaat ggtaacgtcg catcagcccg
    50521 agaatatcgt catacagccc tgtcccgacg tgcggatcaa aaaagacaaa gggttctttc
    50581 gccagttcag ccagcgttac attcgggtta tttgccaggg gatgatcgtg cgggatcatc
    50641 gccataagcg gttcatggac gatgactgcg tgttcaagcg tctccggtaa cgctgtgtta
    50701 cgcagcaatc ccatatccag cgttccttca atgagcggag cgatttgctc gcgagtgttc
    50761 atttcgcggg tttgtaaatg gacatcggga taatctcggc gaaacagcga taacgtatcg
    50821 gacaccgccc gaataaaagg agccgacgaa gtaaaaccaa tgcgcaactc ccccgcttca
    50881 ccctgatgca gtctttcagc gcgagcggca gcgtcatcca ccatagacag gatttgccga
    50941 ctatctgcaa gaaactgttt tcctgctgcc gtcagcaata cactgcgatt ggttcgtgcc
    51001 agcagtcggg caccaatttg ttgctccagc gcctgaatct gctgacttag cggcggttgc
    51061 gaaatattca ggcgggcagc ggcgcgcccg aaatgcagct cttccgcaac agcaacaaag
    51121 taacgcagat gacgaagttc aatattcata tttaaaacat cttatttgag attattaata
    51181 tattagacag aacaattcga ttttcctaac ctatgtataa gcctgatcca caggcatatt
    51241 tagcaaggat ttcaagtgag ccgtactaca actgttgatg gcgctccggc aagcgacact
    51301 gacaagcaaa gcatttctca gccaaatcaa tttattaaac gcggtacgcc gcaatttatg
    51361 cgcgtcaccc tggcgctgtt ctctgccgga ctggcaacat ttgcacttct ctattgtgtg
    51421 cagcctatcc ttccggtgct ttcgcaggag tttggcttaa cgcccgcgaa cagtagtatt
    51481 tcactgtcca tttccacggc gatgttggct attggtttgc tgtttactgg ccccctatcc
    51541 gatgccattg gtcgcaaacc agtgatggtc acggcgctac tgttggcctc catttgtacg
    51601 ttactttcga caatgatgac cagctggcac ggcattttga ttatgcgcgc cttgattggg
    51661 ctttcgttaa gtggcgtggc agctgttggc atgacttatc ttagcgagga aatccacccc
    51721 agtttcgtgg ccttttcgat ggggttgtat atcagcggca actcaattgg cggcatgagc
    51781 ggacgcttaa ttagtggtgt cttcacggac tttttcaact ggcgaattgc tctggcggct
    51841 atcggttgtt tcgcgctagc ctcggcgttg atgttctgga aaatcctccc ggaatcacgc
    51901 cattttcgcc cgacctcgct gcgacctaag tcgttgttta tcaactttcg tttgcactgg
    51961 cgtgaccggg gattaccgtt attgttcgca gaaggctttt tgctgatggg gtcgttcgtc
    52021 acgctgttta attacatcgg ctatcggttg atgctctccc cctggcatgt cagtcaggcc
    52081 gtggttggct tattatcgct ggcttatttg accggtacat ggagctcacc caaagccgga
    52141 accatgacca cccgctatgg gcgcggtccg gtgatgttgt tttcgacggg ggttatgctg
    52201 tttggtttac tgatgacctt attcagctcg ctgtggctga tctttgccgg aatgttactc
    52261 ttctcagcag gattcttcgc agcccactca gtagccagca gctggatcgg cccccgcgca
    52321 aaacgcgcta aaggccaggc ctcctcgctg tatctgttca gttactatct ggggtcgagt
    52381 attgccggga cgctgggtgg tgttttctgg cataactatg gctggaacgg cgtcggcgca
    52441 tttattgctc tgatgctggt cattgctctg ctggtcggga cgcgtttgca tcgtcgtctg
    52501 cacgcctgaa aaataagtcc ggactgcggt aaatacccgt ccggacttat tgcctgctca
    52561 aaccaacgtt aatagccatc ctaaaataga cgaagcgcca gccaattccc gcagcgcgtc
    52621 tagcgtcatc aggattataa gtacccaaat aaacggattc attttgctgt gtgtcattta
    52681 ttactgatgc gcagttattc taccgctttg taagtagtaa aatagttaac ccgatcaaga
    52741 ctactattat tggtagctaa atttccctta agtcacaata cgttattatc aacgctgtaa
    52801 tttattcagc gtttgtacat atcgttacac gctgaaacca accactcacg gaagtctgcc
    52861 attcccaggg atatagttat ttcaacggcc ccgcagtggg gttaaatgaa aaaacaaatt
    52921 gagggtatga caatgaaaaa agtattagct ctggttgttg ccgctgctat gggtctgtct
    52981 tctgccgcct ttgctgcaga gactgcgacc acacctgctc cgactgcgac gaccaccaaa
    53041 gcagcgccgg cgaaaactac acatcataaa aaacagcata aagcagcacc tgcccagaaa
    53101 gcgcaggcgg ctaaaaagca tcataaaaat gcgaaagctg aacagaaagc ccctgaacaa
    53161 aaagcgcagg cagcgaagaa acacgccaag aaacacagcc atcagcaacc ggcaaaacct
    53221 gctgcacaac ccgcagcgta agttttcaac agtaatgctg gcgcgccccc tcgcgcctga
    53281 aaattacggt gctaagcggg taacgtttag caccgccttt agcggagggt agtatggtgg
    53341 gccgttatcg ctttgagttc attcttatca tccttatttt atgcgcactg attgccgccc
    53401 gtttttatct ttcctgactg tagttatctg attttactcc cactttcatc ccgtcccgtc
    53461 tatagtattt atgagggttt gcttttaata atcataatta cccaccagag tgtgatatgc
    53521 gtacaaccat tgctgtagtg ttgggtgcaa ttagtttgac gtctgctttt gtgtttgcag
    53581 ataaaccaga cgttgccaaa tcggcaaacg atgaggtcag caccttgttt tttggtcatg
    53641 atgatcgtgt gccagtgaat gacacgaccc aatcaccgtg ggatgcggtt gggcaactgg
    53701 aaacggccag cggcaattta tgtacggcga cgctgattgc acccaatctg gcattaacgg
    53761 caggacactg tttattgaca cctccaaagg gtaaagccga taaagcggtg gcgctgcgtt
    53821 ttgtgtcaaa taaaggtctt tggcgctatg agatccacga catagaaggc cgcgttgatc
    53881 cgacactggg aaagcggtta aaagcagatg gggatggttg gattgtacct cccgcagccg
    53941 cgccgtggga cttcggattg attgtgctac gtaatccccc ttctggcatt acgccgttgc
    54001 cgttatttga gggagataaa gccgcgctta ccgccgcatt aaaagcggca ggtcgtaaag
    54061 tgactcaggc aggataccct gaagatcatc tcgatacgtt gtacagtcat caaaactgtg
    54121 aagtgactgg ctgggcgcaa acgtcggtga tgtcacatca gtgcgatacc ttgccgggtg
    54181 acagcggttc gcctctgatg ttgcataccg atgacggctg gcaattaatt ggggtgcaaa
    54241 gttcggctcc tgccgcgaaa gatcgctggc gcgccgataa ccgggccatt tctgttaccg
    54301 gttttcgcga caagctggat caactgtcgc aaaaataatg ttcaaacgct gcccgacagc
    54361 gcgggcagcg tcttcatcag gcaagtttca ccatgatcat tccagccaac agcaagacca
    54421 ggccaatcca gcctttacga tttaaccgtt gaccaaacaa gatccaacct gcggctaacg
    54481 tggcggcaat accaaacccg ccccacaatg cataagcgac agacaagtcg atccctttaa
    54541 cggcctgaga aagcgcacta aaggcagcca gcaccgccgc aagggagagc aagccaaata
    54601 ttttgcgacg aaagccgtca gaaaatttca aaaagacgtt agcaacgatt tccagcacga
    54661 ttgccaatgc cagccaggcg gcgtgaaccc attcaaactg cgccatggtt cacctccagt
    54721 tcaggtttac gcgctttacg ggtacctgat tttatcaaca caatcccggc gaccagggtg
    54781 gttaacccgg caattttcat cagcgataaa ctttcgtcga ataacaaaac gctaaacaag
    54841 gtaataaata aaataccgat accttcccac agcgcataag ctacgcctaa ggcgattttt
    54901 ttaacggcga aagagagaaa tatatacgac agagaaatca tcaccagcat taaaataaag
    54961 ccgccatttc cctcactgac gctcgcccat ttcatggaca gcgtaccggt aatttctgta
    55021 gcaatagcca gacctaataa aatccaataa atatacattg tccttctcct gcaagagaat
    55081 tattttaatt ttcgcttaat tcagcgaaac caaagtaaag tggtcaagcc ccgcgcttag
    55141 cgcaagcttt ggcagaagaa aggactaaag cgcgttgcgc cagtgctgct cacctacgag
    55201 caagatagtg gatgaggtac gaagatgggt aaatgtagaa aataacgtcc tgaacaaatt
    55261 gtccataata ttacaattat ccgcagtgtt gcttctcgtc atcgcggatg ataattgtcc
    55321 tcggtagttg aacacgcctg atttgtatca tagcttaaga attaactcaa aatattttca
    55381 tttctttacc tgagcggttt gattttcgtt atgatgacgg agcgaaaaag acattattat
    55441 tagcaaagga agaaaaaacg gggacaagca tggcaaagcc gatcatcacg ctcaatggcc
    55501 taaaaatcgt cattatgttg ggaatgctgg tcattattct ctgcggtatc cgttttgccg
    55561 ccgagatcat cgtgccgttt attctcgcat tatttattgc tgttattctt aacccgctgg
    55621 tgcaacacat ggtccgctgg cgtgtgccgc gtgtactggc agtgtcgatt ttgatgacca
    55681 tcatcgtgat ggcgatggtg ttgctattag cttacttggg ttccgcgctc aacgagttga
    55741 cgcggacgtt accgcaatat cgcaactcta ttatgacgcc gctgcaagcg cttgaaccgt
    55801 tgttgcaacg cgtagggatt gacgtctcag ttgaccagct ggcgcattat attgatccga
    55861 acgcggcgat gacgttgctc accaacttat tgacgcagtt atctaatgcc atgtcatcaa
    55921 tatttttatt gctgctgacg gtgctgttta tgctgctcga agtgccacaa ttgcccggaa
    55981 aatttcagca aatgatggcg cgtccggttg aagggatggc ggcgattcag cgtgcgattg
    56041 acagtgtgtc tcattatctg gtgctgaaaa cagccatcag catcatcacc ggcctggtcg
    56101 catgggcgat gctcgccgcc ctcgatgttc gcttcgcttt tgtctgggga ttgctggcct
    56161 ttgcgcttaa ttacatccct aatattggtt cagttctcgc ggcaatcccc cctatcgctc
    56221 aggtactggt gtttaatggc ttctacgaag cgttgttggt gctggcggga tatctgctga
    56281 ttaatctggt cttcggcaat attctggagc cacgcatcat ggggcgtggg ctgggacttt
    56341 ccacattggt ggtatttttg tcgttgattt tttggggatg gttgttagga ccggtgggta
    56401 tgctgctttc cgtgccgtta acaattattg tcaaaattgc gcttgaacaa acagcgggag
    56461 gtcaaagcat cgccgttctg ttaagcgatc tcaataaaga gtgacggcct ccgcagaggc
    56521 cgtcagggtt acagagcttt caggattgca tccacgctgg ctttggcgtc accaaacagc
    56581 atgtgggtgt tttccttgaa gaacagcggg ttttgcacac cagcatagcc agtgttcatc
    56641 gaacgtttaa agacaatcac gttctgcgct ttccacactt ccagcacagg cataccagca
    56701 atcggactct tcggatcatc ctgcgccgcc gggttaaccg tatcgttagc accaatcacc
    56761 agtacggtat cggtatcagc aaagtcatcg ttgatctcgt ccatttccag cacgatgtca
    56821 tacggtactt ttgcttcagc cagcaatacg ttcatatgtc caggcaaacg ccccgcaacc
    56881 gggtggatac cgaaacgcac gttgatacca cgagcgcgca gtttctcggt aatttcagcg
    56941 acaggatatt gcgcctgcgc gactgccatg ccgtaccccg gagtaatgat cactgaatgg
    57001 gagtttttca gcagttccgc tgtctcttct gcggtgattt cgcggtgctc acccacttcc
    57061 tgatcatcgc cagtagaaga gccgtcggtg ccgaaaccac ccgcaataac gctgataaag
    57121 gaacggttca tcgccttaca cataatgtaa gaaaggatag cccccgaaga accgaccagc
    57181 gcaccggtca caatcagcag gtcgttgctg agcataaagc ccgcagccgc agccgcccag
    57241 ccggagtacg agttcagcat cgacaccacc actggcatat ctgcaccacc gatggaggcg
    57301 actaaatgcc agccgaatac cagcgcaatg gcggtcatta tcagcaatgc cagcacttgc
    57361 aggccgacgc tgtccgtgcg aacaaataca atcagcagca ggaaggaaac gaccagagcc
    57421 gccaggttca ttttgtgacg gtttggcagc atcaacggtt tagacgaaat cttgccacac
    57481 agtttgccga acgccaccac cgaacccgtg aacgttaccg ccccgatgaa gataccgagg
    57541 aacacttccg tcaggtgaat attgaccaga atcggtgcca ttcccgcgtc atgatgcaga
    57601 tagctgttaa agccaaccag cactgccgcc agacccacga agctatgcag gatcgccacc
    57661 agttctggca tttcggtcat ttcaactttc ttcgccagac ggataccaat tgccccacca
    57721 atgaccatcg ccagcaagat ccagccaacg ttacccgtat ccggtccaaa aatggtggcg
    57781 attagcgcaa tcgccatccc ggcgataccg aagttgttac cctggcgaga cgtttcatgt
    57841 ttcgaaagcc cggccagact gaagataaac aggatcgcgg caacaatgta tgcagctgta
    57901 actaatcctc cagacatatg ttacccctta atttttgcgg aacattttca gcatgcgctg
    57961 agtcacggtg aagccaccga aaatattaat gctggctata agcaccgcga taaaactaag
    58021 gaagctaacc cagccgccct ggccaatctg caacagtgct ccgacaacaa taatccctga
    58081 aatcgcgttg gtgaccgaca tcagcggtgt atgcagcgcg tgcgatacat tccacaccac
    58141 gtaataaccg acaacgcagg ccagcgcgaa aacggtgaag tgcccaagaa actctttcgg
    58201 cgcaacgctt gccagccagc cgaaaaggat gattgccagc gccatcaacg cgtatttacg
    58261 ccacggtgaa caggcacatt tttcctcagt tttcacttcc ggtgccgctt tttgtgccgc
    58321 ctgcggctga gctgatacct gaatcggcgg tgccggccag gtaatttcgc ccgcacggat
    58381 cacggtcacg ccgcgaatca ccacatcatc aaaatcaaca gtgatattgc cgtctttctc
    58441 tttgcacaac agtttcagca gattaacgag gttagtaccg taaagctgtg aggattgcgt
    58501 cggcagacgg cccggaagat cggtatagcc aatcactttg acaccatttt ccgtagtgaa
    58561 gatttcaccc ggcacggtgt attcacagtt gccgccgttt tgggctgcca ggtcgacaat
    58621 cacactgccc gccttcatgg agtcaaccat ttcacgggta attagcttcg gcgctggttt
    58681 gcctggaata agcgcggtgg tgacaatgat atcgacctct tttgcctggg cggcaaagag
    58741 ttccatttcc gctttgatga acgcgtccga catcactttg gcatagccat cgccgctacc
    58801 cgcttcctct ttaaaatcca gctcgaggaa ttccgcgccc atactttgaa cttgttcttt
    58861 cacttccggg cgggtgtcga atgcacgcac aatcgcgccg agactgtttg ctgcgccaat
    58921 ggcggccaga cctgcaacac ccgcaccaat caccatcact tttgccggtg gcactttccc
    58981 ggccgcagtg atttgcccgg taaagaagcg cccaaattca tgtgccgctt caacaatggc
    59041 gcgataaccg gcgatgttcg ccatcgagct taatgcgtcc agcgattgtg cgcgtgagat
    59101 acgcggtaca gagtccatcg ccatcacggt cacgttacgt tccgcaagtt tttgcattaa
    59161 ttccggattc tgcgcaggcc agataaaact caccagcgtt gtcccaggat tcagtaacgc
    59221 aatttcatca tctaacggcg cattgacctt cagaatgatc tctgactgcc agacgctatt
    59281 cccttctaca atttcagcgc ccgcttgcac aaacgcttta tcgtcaaaac ttgccagttg
    59341 acccgcgccg ctctctaccg cgacggtaaa acccagtttc agcagctgtt ccactgtttt
    59401 tggcgttgct gcaacacggg tttcattggt taaccgttct cttggtatgc caattcgcat
    59461 gatattccct tccatcggtt ttattgatga tggtttgcct gtgtcaggag ccacacaagc
    59521 cgctcatgta cgagctaaat gttactccgt taaaataaat tagtaacaaa cgccctataa
    59581 cgtactgaaa attatgcctg tgatctagcg ccaaaaatca gtatttcggc gtgaactcgc
    59641 aaaatattaa cgattcagcc gtgatagtgg gataaacacc ttagaacgcc ggataaagac
    59701 tgataattgt cttcgacggt cgggtaaaac gagacatcgc cccggcacga atcactactt
    59761 aacattaaat taacttatac aattcagttg cttcagtagt aatgatgctg atacggctgt
    59821 tttttaagca tagacggtca tttgagcagg attaaaaatg gcttaaggaa tgtgatatga
    59881 aaaatgacgc agacagttac accgtttaaa tgcaataatc agccacgttt ctcgttaata
    59941 acaataccag tacctggttt gcgcaaggcg aaggattatt tttatgaagc ttaagaacac
    60001 cctcctggcg tcggcactgc tttctgctac ggcattctcc gttaacgcag caacagaact
    60061 gacaccggag caagcggcag cggttaaacc ttttgaccgt gtagtggtta ccggtcgttt
    60121 taatgctatt ggcgaagcgg tgaaagccgt ttctcgtcgc gcagataaag aaggtgccgc
    60181 ctctttttat gttgtcgaca cttctgattt tggtaacagc ggtaactggc gtgtggtcgc
    60241 tgacctctat aaagccgatg ctgaaaaagc agaagaaaca agtaatcgcg taattaacgg
    60301 tgttgtcgaa ctgccgaaag atcaggctgt tctgattgaa ccgtttgaca cggtcaccgt
    60361 ccagggcttc tatcgtagcc agccagaagt caatgatgcc atcaccaaag cggcaaaagc
    60421 gaaaggtgcc tactctttct acatcgttcg tcaaatcgat gccaaccagg gcggcaacca
    60481 gcgtattact gcattcatct ataaaaaaga tgctaagaaa cgtatcgtcc agagcccgga
    60541 tgtgatcccg gcagattccg aagcaggacg tgcagctctg gctgccggtg gcgaagccgc
    60601 gaagaaagtt gagatcccgg gtgttgcgac taccgcatca ccaagttctg aagtcggtcg
    60661 cttctttgaa acccagtcat caaaaggcgg gcgttacacc gtcacgctcc cggatggcac
    60721 taaagtcgaa gaactgaaca aagcgaccgc agcgatgatg gtcccgttcg acagcatcaa
    60781 attctctggc aactacggca acatgaccga agtctcctat caggttgcga aacgtgccgc
    60841 gaagaaaggt gctaagtatt accacatcac ccgccagtgg caggaacgtg gtaataacct
    60901 gaccgtcagc gcagatctgt ataaataact attcagttac tcctataagg cggcttgatg
    60961 ccgcctttcg catttttagt cacaaatttc cctgcgacca ttgcatgcag acacatctct
    61021 tcgtaaaatc ccgcgcttca gtggcaaact ccatttttat gcgttttgcc ctaatattta
    61081 ttcttcttat cacgttttaa tcactggata tcgatggaaa agaaactggg actgagcgca
    61141 ctcaccgcgc tggtattaag ctcaatgctg ggcgcgggtg ttttcagtct gccgcaaaat
    61201 atggcggcag ttgccagccc ggcagcactg ctcatcggct ggggtattac tggcgctggc
    61261 attttattgc tggcctttgc catgctgatc ctcacgcgca ttcgtcccga acttgacggc
    61321 ggtatcttta cctatgcccg cgaagggttt ggcgagctaa tcggcttttg ttccgcatgg
    61381 ggatactggc tgtgcgcggt catcgccaac gtctcctatc tggtgatcgt tttttccgcg
    61441 ttaagctttt ttacggacac gccggaattg cgcctgtttg gcgatggtaa tacctggcaa
    61501 tcgatagtcg gtgcatcggc tttattgtgg attgttcact ttttgatcct gcgcggggtg
    61561 caaaccgctg ccagcattaa cctggtggcg acactggcaa aattgttgcc gctgggtctg
    61621 tttgttgtgc tggcgatgat gatgttcaaa ctggatacct tcaagctcga cttcaccgga
    61681 cttgcacttg gcgtacctgt ttgggaacag gtgaaaaaca ccatgctgat caccctgtgg
    61741 gttttcattg gtgtggaagg tgccgtggtt gtttctgcgc gtgcgcgtaa taaacgtgat
    61801 gttggcaaag cgacactact ggcggttctc tccgctctgg gcgtttactt gctggtaacg
    61861 ctgctttcgc tgggtgtggt ggcacgtcct gaactggctg aaattcgtaa cccatcaatg
    61921 gccggtctga tggtggaaat gatgggacca tggggcgaaa tcatcatcgc tgccggtttg
    61981 atcgtttccg tttgcggcgc gtacttaagc tggaccatca tggcggcaga agttccgttc
    62041 ctggctgcca ctcataaagc attcccacgc atttttgcgc gccagaacgc acaagctgcg
    62101 ccatccgcct cactgtggtt gaccaacatc tgcgtgcaaa tttgtctggt actcatctgg
    62161 ctgaccggtt ccgattacaa cacgttgctg accatcgcct cagaaatgat tctggtgccc
    62221 tatttcctgg tcggcgcgtt cttgctgaaa atcgcaaccc gtccactgca taaagcggta
    62281 ggtgtcggtg cctgcattta tggcttatgg ttattgtacg cctctggtcc aatgcacttg
    62341 ctgttatccg ttgttctgta tgcaccgggg ctgctggttt tcctgtatgc gcgcaaaacg
    62401 catacacatg ataatgtact gaaccgtcag gaaatggtgc tgattggcat gctgttaatt
    62461 gcctcagttc cggcgacctg gatgctggtg ggataacgtt catcccatcg ttggatagta
    62521 aaggagataa cgatgggtaa aacccagccc ttgccaatat taattactgg cggaggtcgt
    62581 cgcatcggcc tcgcccttgc atggcatttc attaatcaaa agcaaccggt gattgtcagc
    62641 tatcggacac actatccagc cattgatgga ctgattaatg caggtgcgca gtgtattcag
    62701 gctgattttt cgaccaacga cggtgtgatg gcgtttgccg atgaagtact aaaaagcacc
    62761 catggtctgc gtgctatttt gcataacgcc agtgcgtgga tggcggaaaa accgggtgcg
    62821 ccactggccg acgtactggc ttgcatgatg cagatccacg ttaatacccc atacctgctc
    62881 aaccatgcgc tggaaagatt actgcgtggg cacggacacg ccgccagcga tatcattcac
    62941 tttaccgatt atgtggtgga gcgcggtagc gacaagcata ttgcgtatgc tgcaagcaaa
    63001 gcggcactgg ataatatgac ccgctcgttt gcccgcaagc tggcaccgga agtgaaagtg
    63061 aattctattg cgccatcgct gatcctgttt aatgaacatg atgatgccga atatcgacaa
    63121 caggcgctga ataaatcact gatgaaaacc gcgcctggcg agaaagaagt gatcgacctg
    63181 gtcgattact tacttaccag ttgctttgtc accggacgca gtttcccact tgatggcggt
    63241 cgtcatctgc gttaatgcag ctttatccag catataatca acacccatgc gcttatcccc
    63301 caacacgcaa cagaaccaac aaaagcggcg ggcaagcgca tcattccggt gaaataccac
    63361 agcgacacca ggtaaacaaa atagggaata atcgaccaca tactaaaaat gatggttgcg
    63421 cgtaaggctt caatgccgcg ttcgctggca acaatataat gcgcgatgag cgcaaaggtc
    63481 gggaaaagtg gaatcagccc ggcgatataa taatttttcg tttttgctaa aacaccaatc
    63541 aacagcacta ccagcgcacc gagcgcggct ttgattacca gccccatctt tttaccttaa
    63601 cacttccata acaagtcatc agtagaatac ctgatgaaaa cttgtttaga aacgattgat
    63661 agtaagtaaa aacagcgcgg tgtattgtga cgtttttata tctaccgtga atgttatgaa
    63721 cactatcgta tttgtggaag atgatgcgga agtcggttca ctgattgccg cgtacctagc
    63781 aaaacatgat atgcaggtta ccgtagagcc gcgcggcgac caggccgaag aaaccatttt
    63841 gcgagaaaat ccggatttgg tgttactcga catcatgcta ccaggcaagg acggcatgac
    63901 catttgtcgt gatttacgcg caaagtggtc tggaccgatt gttcttctaa cctctctcga
    63961 tagcgatatg aaccacatcc tggcactgga aatgggtgcc tgcgactata ttctcaaaac
    64021 gacgccccct gctgttttgc tagcgcgttt acgtttgcat ttgcgtcaga atgagcaagc
    64081 cacactgacc aaaggtcttc aggaaacgtc tctgactccc tacaaagccc tgcatttcgg
    64141 cacgttgacc atcgatccca tcaaccgcgt agtcaccctg gctaacactg aaatctcgct
    64201 ctcgacagct gatttcgaat tattgtggga attagctacc catgccgggc aaatcatgga
    64261 ccgcgatgca ttgctgaaaa atttacgcgg cgtcagttat gacggcctgg atcgtagcgt
    64321 ggacgtggct atttcgcggt taagaaaaaa actgctcgat aacgccgcag aaccttatcg
    64381 cattaaaact gtgcgtaaca aaggctatct ctttgcgcct catgcatggg aataagcgat
    64441 gaaaaaactg tttatccagt tttacctatt attgtttgtc tgcttccttg taatgtctct
    64501 gctggttggg ctggtgtaca aatttaccgc cgaacgcgcg ggcaaacagt cgctggatga
    64561 tttgatgaac agttcgctgt atctgatgcg cagcgaattg cgtgagatcc ccccacacga
    64621 ctggggtaag acgctgaaag agatggattt aaatctctct ttcgatctgc gtgtcgagcc
    64681 actgagtaaa taccatcttg atgatatttc catgcaccga ctgcgtggcg gcgaaattgt
    64741 cgccctggac gatcagtaca cgtttttgca gcgtatcccg cgcagccact acgtgctggc
    64801 agttggtcct gttccttatc tttattacct ccatcagatg cgattgctgg atatcgccct
    64861 gatcgctttt attgctattt ccctcgcctt tccggtgttt atctggatgc gtccgcactg
    64921 gcaggatatg ttaaaactgg aagcagcggc gcaacgattt ggcgatgggc atctcaatga
    64981 acgtatccac tttgatgagg gttcgagctt tgaacgactt ggtatcgcat ttaaccagat
    65041 ggcggacaat atcaacgcct tgattgccag caaaaaacag cttattgacg gtatcgctca
    65101 cgaactgcga acaccgttag tgcgcctgcg ttatcgactg gagatgagcg ataacctgag
    65161 cgccgccgaa tcccaggcgt tgaatcgtga tatcagtcaa cttgaagctt taattgaaga
    65221 gctgctgact tatgcccgac tcgatcgccc acaaaacgag cttcatctta gcgaaccaga
    65281 cctgccgttg tggctatcaa cgcatctggc agatattcag gcagtaacgc ccgataaaac
    65341 ggtacggata aaaacgctca tgcaaggcca ttatgcggcg ttggatatgc gcttaatgga
    65401 gcgcgtgctg gataatttgc tcaataacgc cctgcgctac tgccattcaa cggttgaaac
    65461 cagcctgcta ctgtcgggga atagagcgac attaattgtt gaggatgatg gcccagggat
    65521 tgccccagaa aaccgcgaac atatctttga accttttgtt cgcctcgatc ccagccggga
    65581 tcgctcaacc ggcggctgcg ggctggggct ggcaattgtc cactctatag cactggcaat
    65641 gggcggtacg gttaattgtg acaccagcga actgggtggt gcccgcttct cgtttagctg
    65701 gccgttatgg cataacatcc cgcaatttac ctctgcttga cactacgcgc acggtggtca
    65761 agtcaccacc actgtgctat aaattaagga tgttgtaact aaagtggtta atattatggc
    65821 gcgttacgat ctcgtagacc gactcaacac tacttttcgc cagatggaac aagagctggc
    65881 tgcatttgcc gctcatcttg agcaacacaa gctattggtt gcccgcgtgt tctctttgcc
    65941 ggaggtaaaa aaagaggatg agcataatcc gcttaatcgt attgaggtga aacaacatct
    66001 cggcaacgac gcgcagtcgc tggcgttgcg tcatttccgt catttattta ttcaacaaca
    66061 gtccgaaaat cgcagcagca aagccgctgt ccgtctgcct ggcgtgttgt gttaccaggt
    66121 cgataacctt tcgcaagcag cgttggtcag tcatattcag cacatcaata aactcaagac
    66181 cacgttcgag catatcgtca cggttgaatc agaactcccc accgcggcac gttttgaatg
    66241 ggtgcatcgt catttgccgg ggctgatcac ccttaatgct taccgcacgc tcaccgttct
    66301 gcacaacccc gccactttac gttttggctg ggctaataaa catatcatta aaaatttgca
    66361 tcgtgatgaa gtcctggcac agctggaaaa aagcctgaaa tcaccacgca gtgtcgcacc
    66421 gtggacgcgc gaggagtggc aaagaaaact ggagcgagag tatcaggata tcgctgccct
    66481 gccacagaac gcgaagttaa aaatcaaacg tccggtgaag gtgcagccga ttgcccgcgt
    66541 ctggtacaaa ggagatcaaa aacaagtgca acacgcctgc cctacaccgc tgattgcact
    66601 gattaatcgg gataatggtg cgggcgtgcc ggacgttggt gagttgttaa attacgatgc
    66661 cgacaatgtg cagcatcgtt ataaacctca ggcgcagccg cttcgtttga tcattccacg
    66721 gctgcacctg tatgttgcag attaacgccc ggctttcata ctgccgacca tctgttctgg
    66781 ccgtacccag ctgtcaaact cggcttcgct aagatacccc agcgcaaggg ccgcagcttt
    66841 taaggtcagc ccttctttat gcgctttttt ggcgatctcc gcggctttgt cataaccaat
    66901 gtgggtgtta agcgcagtca ccagcatcag cgattcattg agtaattgat tgattcgctc
    66961 acgattcggt tcaataccca ctgcgcagtg tttgttaaaa ctttccatgc catctgccag
    67021 caagcgcacc gattgcagga aattatggat caccatcgga cggaagacgt tcagttcaaa
    67081 gttaccggaa gcgccaccca tgttgatcgc cacgtcgttc cccatcacct gacagcagag
    67141 catggttaat gcctcacact gtgttgggtt cactttccct ggcatgattg agctgcccgg
    67201 ctcattttcc gggattgaga tttcaccaat tccgcagcgc gggccggagg ccagccagcg
    67261 gacatcattg gcgattttca tcagtgacgc agccaaccct ttcaacgcgc cgtgcgcctg
    67321 aaccagggca tcacaggtcg ccagcgcttc aaatttgttc ggcgcggtaa caaacggtgc
    67381 acaggtaatg actgccagtt catctgctac gcgacgcgca tactccggat gggtatttag
    67441 tccagtaccc accgctgtac cgccaagagc cagttccgct acgtgaggca ggctgtattc
    67501 gatatgtttg agattatgct cgagcatcgc tacccagccg gaaatctcct gccccagcgt
    67561 taacggcgtg gcatcctgca agtgagtacg accaattttg acgatatcgg caaatgcacg
    67621 ggatttctca ttcagtgtct gtgtcagggt tttaagctgc ggaatgagtt gcttgcgcag
    67681 cgccagcagc gccgcaacgt gcatcgccgt cggaaagaca tcgttggaac tttggctttt
    67741 gttcacgtca tcgttagggt gaactttacg ttccatcccg cgcacaccgc cgagtaattc
    67801 actggcccgg ttagccagca cttcgttcat gttcatgtta ctttgcgtgc cggagccggt
    67861 ctgccagata gccagcggga attcgtcgtc atgctgtcct gccagtactt catccgccgc
    67921 ctgacgaatg gcgctcgctt tctcttcaga caacaagcct aaatcttcat taacttttgc
    67981 cgctgcacgc ttggttagcg ccagcgcatg aatcagtgag gtgggcattt tctccgtcga
    68041 aatgcggaaa tgctccagcg agcgttgagt ttgtgcgccc cacagcttat ctgccgggac
    68101 atcaatcgcc cccatcgaat ctttttcgct gcgtactgta ttcatggcct gctcctcacc
    68161 tgattaattt tttctttctg ttttgctttc gttaagcaac ttttagctca cttattattt
    68221 accatttgat aacaaatgtt tggtctttcg tgccatgtaa aaaaaccgcc ccgaagggcg
    68281 gctctgttta tttcacacag cgggtgcatt gtgtgagttg tatctgctga aagaagtcat
    68341 ttcctttatc atccacaaga ataaacgccg ggaaatcttc cacttcaatt ttccagatgg
    68401 cttccattcc cagttccgga tattcaacac attccaggct cttaatactt ccctgcgcca
    68461 atacagcggc cggaccaccg atactgccaa ggtagaagcc gccgtgtttt ttacaggcat
    68521 ccgtcacctg ctggctgcgg ttgcctttcg ccagcatgat catacttccg ccctgcgctt
    68581 gcagttgatc gacataagaa tccatccgtc cggcggtcgt tgggccaaga gaaccggagg
    68641 cataaccttc cggcgttttg gccggacccg cgtagtaaat cggatgatct ttaatgtact
    68701 gcggcagccc ttcaccgtta tccatccgct ctttcagttt ggcgtgggca atatcacgac
    68761 caacgataat cgtgccgtta agcgataagc gtgtagaaac gggatactgc gacaactgtg
    68821 cgaggatctc tttcatcgga cggttaaggt caacgcgcac cgcttcgcct tctcccgctt
    68881 tgcgcagctc ttccgggata tatttgcctg gattatgttc cagtttttcg atccagatcc
    68941 cctgacggtt gatcttcgct ttgatattac ggtcagcaga gcaggagacg cccataccga
    69001 ccgggcagga tgcgccgtga cgtggcaggc gaatcacgcg gatgtcgtga gcgaagtatt
    69061 taccaccaaa ctgcgcaccc agaccaagat tttgcgcttc gatcagcaat tctttttcca
    69121 gttccacatc gcggaacgcc tgaccgtgct cattcccttc cgttggcagt tcatcatagt
    69181 atttcgcgga agccagtttc accgttttaa ggttcgtttc tgcagaagtt ccaccaataa
    69241 cgaacgcaat atgatacgga ggacaggccg ccgtacccag cgtgcgcatc ttctcaacca
    69301 ggtaattttt cagtttcccc ggcgtcagta acgctttggt ttcctgatag agatacgtct
    69361 tgtttgccga accaccacct ttggcgatac agaggaattt gtactcgtcg ccatcaacgg
    69421 cataaagatc gatctgcgct ggtagattgg tgccggtatt cacttcttta tacatatcca
    69481 gcggcgcgtt ttgcgagtag cgcagattat cttcgatata agtgttatag acaccgcgcg
    69541 ccagcgccgc ttcatcacca ccaccggtcc atacacgctg ccctttttta ccaacaataa
    69601 tcgcggtgcc ggtatcctga caggttggca gaacgccttt cgccgcgatg tcggagttac
    69661 gcaggaattg cagcgccaca tatttatcat tttcgctggc ctccgggtca cgcagaatgt
    69721 cggccacctg ttgttggtgc gccggacgca gcataaacga cgcatcatga aacgcctgac
    69781 gcgccaacag agttaacgct tcgggtgcga ctttcaaaat ctcctgccct tcaaattcag
    69841 atacgctaac gtgttcgctg gttagcaggt aatactcagt atcatctttt ttgagtggaa
    69901 aaggagcctg ataatgaaag ggtttgtttg acattgttct ctcacttact gcctggtttg
    69961 gttatgctct gggcgggtgt tccgttgccc tgttaaaagc gagtaacaat atcctacaca
    70021 cttttttaac aaaaactgag actagtacga ctttttgcgg ctccaggtta cttcccgtag
    70081 gattcttgct ttaatagcgg gattaatttc cacattaaaa cagggattga tcatgcaaaa
    70141 actcattaac tcagtgcaaa actatgcctg gggcagcaaa acggcgttga ctgaacttta
    70201 tggtatggaa aatccgtcca gccagccgat ggccgagctg tggatgggcg cacatccgaa
    70261 aagcagttca cgagtgcaga atgccgccgg agatatcgtt tcactgcgtg atgtgattga
    70321 gagtgataaa tcgactctgc tcggagaggc cgttgccaaa cgctttggcg aactgccttt
    70381 cctgttcaaa gtattatgcg cagcacagcc gctctccatt caggttcatc caaacaaaca
    70441 caattctgaa atcggttttg ccaaagaaaa tgccgcaggt atcccgatgg atgccgccga
    70501 gcgtaactat aaagatccta accacaagcc tgagctggtt tttgcgctga cgcctttcct
    70561 tgcgatgaac gcgtttcgtg aattttccga gattgtctcc ctactccagc cggtcgcagg
    70621 tgcacatccg gcgattgctc actttttaca acagcctgat gccgaacgtt taagcgaact
    70681 gttcgccagc ctgttgaata tgcagggtga agaaaaatcc cgcgcgctgg cgattttaaa
    70741 atcggcgctc gacagtcagc agggtgaacc gtggcaaacg attcttttaa tttctgaatt
    70801 ttacccggat gacagcggcc tgttctctcc gctgttgctg aatgtggtga aattgaatcc
    70861 tggcgaagcg atgttcctgt tcgctgaaac accgcacgct tacctgcaag gcgtggcgct
    70921 ggaagtgatg gcaaactccg ataacgtgct gcgtgcgggt ctgacgccta aatacattga
    70981 tattccggaa ctggttgcca atgtgaagtt cgaagccaaa ccggctaacc agttgttgac
    71041 ccagccggtg aaacaaggtg cagaactgga cttcccgatt cctgttgatg attttgcctt
    71101 ctcgctgcat gaccttagtg ataaagaaac caccattagc cagcagagtg ccgccatttt
    71161 gttctgcgtc gaaggcgatg caacgttgtg gaaaggttct cagcagttac agcttaaacc
    71221 gggtgaatca gcgtttattg ccgccaacga atcaccggtg actgtcaaag gccacggccg
    71281 tttagcgcgt gtttacaaca agctgtaaga gcttactgaa aaaattaaca tctcttgcta
    71341 agcttattaa aggcttataa caccttcagg cggccagtcc gcctgatttc attttatgga
    71401 taatcattat gaataaatcg ctggtagcgg taggcgtcat tgttgcgcta ggcgtagtct
    71461 ggacaggcgg cgcatggtat acaggcaaga agattgaaac ccatctcgaa gagatggtcg
    71521 cgcaggcgaa cgcgcaactc aaactgacag ctcctgaatc caacctggaa gtgagttatc
    71581 aaaactatca tcgcggcgta ttcagcagcc agttgcaact gttggtgaaa cccattgccg
    71641 ggaaagaaaa tccgtggatt aaaagcggtc agagcgtcat cttcaacgaa tcggttgatc
    71701 atggtccctt cccccttgcc cagcttaaaa aactgaacct gatcccatcg atggcatcaa
    71761 ttcaaaccac gctggttaat aacgaagtaa gcaaaccact gtttgatatg gcaaaaggtg
    71821 aaacgccttt tgagattaac tcgcgcattg gttacagcgg tgattccagt tccgatattt
    71881 cgctcaagcc gctgaattac gagcaaaagg atgaaaaagt cgcctttagc ggcggcgagt
    71941 tccagttaaa tgcggacaga gacggcaaag ctatctccct ttccggggag gcgcaaagtg
    72001 gtcggataga cgcggttaac gaatacaacc agaaagtaca gttgaccttt aataatctga
    72061 aaaccgacgg ttccagcacg ctggcaagtt ttggtgagcg cgtaggaaac caaaaactgt
    72121 cactggaaaa aatgaccatt tcagtggaag gcaaagaact ggcactgctg gaaggcatgg
    72181 agatcagcgg taaatcggat ctggtcaatg acggtaaaac gatcaatagc caactggatt
    72241 actcgctaaa cagcctgaag gtacagaatc aggatctggg cagcggcaag ctgactttaa
    72301 aagtcggcca gattgatggt gaagcctggc atcagtttag ccagcaatat aacgcgcaaa
    72361 ctcaggcgct gctggcacag ccagaaattg ccaacaatcc cgaactttat caggagaaag
    72421 tgacggaagc cttctttagc gccctgccgc tgatgttgaa aggcgatccg gtgattacta
    72481 tcgcgccgct aagctggaaa aacagtcagg gtgaaagtgc gctgaatctg tcgctgttcc
    72541 tgaaagaccc ggcaacgact aaagaagcgc cgcaaacgct ggcgcaggaa gtagatcgtt
    72601 cggttaaatc tctggatgcg aaactgacca ttccggtgga tatggcaact gagttgatga
    72661 ctcaggtagc gaagctggaa ggttatcagg aagatcaagc gaaaaaactg gcgaaacagc
    72721 aagttgaagg tgcatcagca atggggcaga tgttccgtct gaccaccttg caggacaata
    72781 ccatcaccac cagcctgcaa tatgctaacg gtcagataac gttaaatggg cagaaaatgc
    72841 cactggaaga tttcgttggt atgtttgcaa tgccggcatt aaacgttccg gtcgtacccg
    72901 ctattccgca gcagtaattc acagccaaaa cgccggaggt ttttctctcc ggcgttattt
    72961 cttacttcag cataaagtca tactgaatac tcattttcac atcccagtaa ttcaggcgat
    73021 cgcccagcgt actgccatcg cgcttttccc gccctttatc ggcagcaaga aagaaattca
    73081 tcccctgtaa cggcccttcg ctgacccgat agttcatacc acctaagata cccgcgcctt
    73141 caatccgcac atctcgttta ctggattcat agccgcgagt cacaatcggt gcaaatgtca
    73201 gcgtaaattg cggggttaaa cgatagttga cgcccaattg ccaggactgc attccttcac
    73261 ggttacgatc aagacttaat gaaaagggat agccaatgtc ggtatccacc acggcaccat
    73321 tcattgcatg gcgcaaataa ccaccactgc caaatacgct tccccacggc gcattccagg
    73381 tcaattgtcc actaaccaac gccgtttcat tgggctggct ggtattacga cttaaacctt
    73441 cgagctgcgc gtaaaagccg agcaactccc ctttcaacgt ataattcggg tcgagcggta
    73501 tgcttttctc gagaatactt tgtatctgat tggaaacatc tttctgtacc gatgccccca
    73561 gagaatagcg actggtatca tcatgccaac tggcggctaa aaaagaacgt gcgcgatgtt
    73621 tatcgtattt tcccgtctgg ctgttataca ccccttcatc acgaataccg gttgttgaat
    73681 cagtccataa aaatactcgt gaacgccaca ttccccacag atcaatattt ggcgttaaag
    73741 cccaataacc taatgctcct tcagaaactg tcggtaatgt acgttgcgaa ctgccagcca
    73801 ttaacggaat acgataaggt aagctaccat aactataatc cgtcccgaag cgacctaatt
    73861 taagcaggct attttcactg ggttttattt ttactgcccc cagggcaaag ccaaaacttt
    73921 tatcaccatc aagataccac cgggtactca tatcagcacg agcacctaat ttataaacat
    73981 aatacgctcc gccttcaacg ccaataatat tggaataata accactattg aaatcgagca
    74041 atcctccctg tacccatgcg taaatatctc ttcccgcgcc agcactgggc ttatcagccc
    74101 tgcgcaattc gtttttcagt ctgatgcgta gtcccgcttc atcgtcagcc agttgcgccg
    74161 cgtaagcaga ggtaaggcca gaggcagccg tcaggcaaat aacggccatg gccactattt
    74221 ttctcatagt tgcacctttg atgttctgat tatttttatt gaatattaat tagtgatatc
    74281 gctgattaat tgctgctgca cttttttacg attatcaatt tcaaccacga tttctttgaa
    74341 ttttttatcc gtgagcggat aaaaccagat aataacgaat gccagtagca taaatccgca
    74401 aggtactaag gcaattgatg tgcgaatgcc cataataact tccggcgttt gcacctgatt
    74461 ggcgatatat ccgcttaacc ccaaaataaa ggcaggaatt gaacctccga ttgcctgacc
    74521 gcatttacgg gtaaatgaga atagtgaata ggtgagccct tcaattcgca cgccggtcag
    74581 gtattcaccg tattctacgg tatcagcttc cagcgcccac ataacagtca tggtaacgcc
    74641 ctgaccaatt gaagcgatgg ccaacgcaac caacgccacc ggcagtgacc agacggaaac
    74701 ccagaagaat agcagataac cgcaggttcc cagcaaagcg ccaatcagga aggtattctt
    74761 tttaccgatc ctcgcgacca tccccggcac caacggtgcc gatgccacag taccaaccag
    74821 gttttgcacc agtaccagca cagtgaacag cccggtatca tttaacacat agcgcacgta
    74881 gaacaacgac gaggcgctga ccgcgaaggt cgaaatcagc acacacagcg caccgatgca
    74941 caacataaac agcgggcgat tccgtttcag ggtttgcaga ctgatcttca atgacggctg
    75001 cgccacgata cgtaccacat tctcacgcgt cgatttgaag cagatgaagt aaagcaccat
    75061 tccggcaatc gccagcacaa tcgtccagaa atggtatacc gacaccatct cttccgggct
    75121 ggagttctta atgctcggtc ctatcagaaa tgccaggcag acaaaggtca atgaagcggc
    75181 aatcccacga gccgcgccca gacgggcgcg ggattgtggt tgttgggtca tcgcggtagc
    75241 aagtgaacca taaggaatat tcaccaggct gtagcaaagc ccgaggccca tgtaggtcaa
    75301 atatgcatac accactttgc taccatggct ccagtcggtc ggcacccaga ataccagcac
    75361 gctgaagatc attaacggcg cagtaccgaa gagtaaaaac gggcggaatt ttccccagcg
    75421 ggtattcaca ctgtccacca ctcgtccggc aaagacgtcg gcgaaggcat cgaatacccg
    75481 caccagtaac agcatggtgc ccgccgcagc ggcaccgacg ccagcgacgt cggtgtagta
    75541 acttagcagg aagagcgccc ccatagcgaa ggcgaagtta ttggcgacgt caccgaggct
    75601 gtagccaacg atggtgcgcc aggagagttg ttgattcatt gtttgcctcc ctgctgcggt
    75661 ttttcaccga agttcatgcc agtccagcgt ttttgcagca gaaaagccgc cgacttcggt
    75721 ttgcggtcgc gggtgaagat gcccttcttg ttaccgccaa cgcgcaatat gccttgcgag
    75781 gtcgcaaaat cggcgaaatt ccatacctgt tcaccgacga cggcgctgac gcgatcaaag
    75841 acgcggtgat acatatccag ccatgcacac tgatactctt cactccacat gtcggtgtac
    75901 attgagtgca gcccggctaa cgtatccacg ccgtattcgg tgatgataat cggctgatgc
    75961 agtttctcct gccaggccag aagttctttt tccagtacct tctctgccgt ttccaaatcg
    76021 ccgctttgga cataccatcc gtaataacgg ttcaggcaca gcacatcaaa gagatcgctg
    76081 atggtatcgg tgtgagcgtc gcagaacatt acattgacgc aggtgatcgg acgcgtcggg
    76141 tcgagtttac gcgttgcttc cgccagtggc gcgaaatatt cccgtgcacc ttgcggacgg
    76201 gtatccggtt cgttggcaat actccacatc accacgcttg ggtggttttt gtcacgcgct
    76261 atcagctctt taatcgcctg taagtgcgcc tgctgagttt ccccgttgac tgcctcttcg
    76321 ctgtacagtt ctttcggctt gttgcccgct tcgaaaccaa tgcctaaaga gaggttaaag
    76381 ccaacagctg cagtttcatc aatcaccacg atgccatgtt catctgccca gtcgagcatc
    76441 tcttcggcat aagggtaatg cgaggtacgg taggagttgg ccccaatcca gtccattaat
    76501 gcgtgatcgt gcaccatcag cacgttatcg aatcctttgc cgcgcaaatc cgcatcttca
    76561 tgacggccaa agccagtaaa gtagaacggt ttgtggttga tcaggaactg ttcgcccttc
    76621 actgccactg accggatgcc gacgcgcagc gggtagatat cacactctgt ctggcttttg
    76681 gctgtgacgc acagttcata gagataacct tcacccggtt gccagaggtg cggattcacc
    76741 acttgcaaag tcccgctggt gccttgtcca gttgcaacca cctgttgatc cgcatcacgc
    76801 agttcaacgc tgacatcacc attggccacc acctgccagt caacagacgc gtagttacag
    76861 tcttgcgcga catgcgtcac cacggtgata tcgtccaccc aggtgttcgg cgtggtgtag
    76921 agcattacgc tgcgatggat cccggcgtag ttaaagaaat catggaagta agactgcttt
    76981 ttcttgccgt tttcgtcggt aatcaccatt cccggcggga tagtctgcca gttcagttcg
    77041 ttgttcacac aaacggtgat acgtacactt ttcccggcaa taacatacgg cgtgacatcg
    77101 gcttcaaatg gcgtatagcc gccctgatgc tccatcactt cctgattatt gacccacact
    77161 ttgccgtaat gagtgaccgc atcgaaacgc agcacgatac gctggcctgc ccaacctttc
    77221 ggtataaaga cttcgcgctg ataccagacg ttgcccacat aattacgaat atctgcatcg
    77281 gcgaactgat cgttaaaact gcctggcaca gcaattgccc ggctttcttg taacgcgctt
    77341 tcccaccaac gctgatcaat tccacagttt tcgcgatcca gactgaatgc ccacaggccg
    77401 tcgagttttt tgatttcacg ggttggggtt tctacaggac gtaacataag ggactcctca
    77461 ttaagataaa aatactggtc aacctttaat ccgattagtt aaccaattct gtgttgcaca
    77521 cagttatctg tgagaaaccc atggaagaga aatggaaata aaaagaactg acaccgcgat
    77581 caaaaaacca aaaactgcaa tacccacagc gtgatataga tcgcattaat ctttaaaacg
    77641 gcgttgacag aagccataaa ataagcaatc gaacgttcgt tcgagcatat tgggaacgat
    77701 aaactctcat gacatatggc tacagtgaat attttaggga ggaattcccc gctccggcga
    77761 gattgtattt tgtacaggga aattaaagta tgaaacgcgg gctcagttgt caccagcccg
    77821 cggaaagaga gagagaatca ggatgcggtt aagataccgc caatcatcgc gcgtaatccc
    77881 tgagcaaacg acgcttcacg cgccaggtct tccgcttcga tatcggccag tacaccgtag
    77941 gtcagatcca gcagtaaacg tgaagtcatt gccgtgttga tgtctgggct tatctcgcct
    78001 ttttgctggg catcacgcat ccgctgagca acaaattcag tgatcgtcat atgcttattt
    78061 ttaagcatgg cggcgacctg tgggttacgc atcccttccg ccataatttc gaccaccagc
    78121 gcccgttgcc caaaggcttc atgggtgaga gagacaatag actcgaccat atagtcaaca
    78181 aaatgaatcc cttcaatcgg ttcccggaaa cgggccagcg ccctctcctg gtcctgtaag
    78241 ataatcgcct gaatcaaggc ttctttggag atgaaatggt gatagagcgt cccgggacta
    78301 atggcgcagg atttacagat ggctttcatc gaggcactgt gaaatccatt ttctgaaaaa
    78361 atctctctgg cagcattgag gatccgggtc cgtgtcggtt gtgcttcagt ctgcatgtta
    78421 tccatcatta aaccattata gttagcgtga ataactagtt taccgcgttg tcaggtgagc
    78481 gcctatcatt gctgttgaat agtgcgaagg cacactctat tcgtatgctt ttcatccagc
    78541 tattggttaa ccaatagccg tgatttggct gccagttgtt tagcagagac gttgatgaat
    78601 tgcacacgct atcccggtaa acaacacata cggctgttta ccggttcgtt agtgtattaa
    78661 ttgagctcct gtaccccacc accggagacg gtgagaattt gtccgcttac ccagctggct
    78721 gcaggcgagc aaaggaacag cgctgcgtta gcaatatctt gcggttggcc cagacgtctg
    78781 atcggcgtgt gctgtaacat tttttgttca atttctggtg taataacgga tttcagggca
    78841 tcggttaata ttgcccccgg cgcaatgcca ttcacccgaa tatttttttc acccaggtca
    78901 aatgccatat ttctgaccag atgactggcc gcagctttag atgatgcata ggaagtcatg
    78961 tttatatttt tattttctgc cgccatagaa gtgatggtaa gaataacgcc accgccattt
    79021 ttttccattt ctggcgcaac aagttgtgac agatggaaaa aagaaaacac attcagttca
    79081 taagcacggc gaaaatccgc cattggcata tcaaacggtt taggtccacc gccaccggcg
    79141 ttgttaacca gaatatcaac tttacccagc ttactgatag caaagtctgc cagtgcagag
    79201 agttcctgtt cggaagtaat atcacaacgg caggcaaatg cctgaccacc cagttgttga
    79261 atttcgtcta caacatggtt agctgcgtcg gcgttaatat cactgaccac cacagatgcg
    79321 ccagctgtcg cgaatgtaat ggcgatttct ttaccaatac ctgcacccgc acctgtgatg
    79381 atggcgcatt ttccgtcgag tctcaggttg tcagaattaa acacataaac ctcctgtgaa
    79441 cttcagtaaa aataaaaggc accgcctgtg tgccacattt tttatcatta accttgagga
    79501 aaggacacta ccaggtatag cagataaaga gaggattatt tcgccgcaat gagccgggcg
    79561 ggaataataa gattgcgtga atgcgtctct tcatgggtga ttttttgcat catgcgatcc
    79621 gcaagtgtgg taccaagttc ccgcgcaggc gtgctggccc aggtaacggg tatatcatca
    79681 agtgtggttg gtgtcgcatc ggtaaatgcc gccagcgaaa cctgttgctc aaagtaacga
    79741 tcgactccgc tttccccgct ttgccgccct gctttcagca aaccaaacca tgcccccatc
    79801 gcaatagttt cgttatagca aaccacggca ctgatggtcg ggttatgacg taaaagcgcc
    79861 gtgatagctt ccgcggcttg cttctggctg gaagtgcact ccaacaccca atcgctgtga
    79921 aacggcaggc caaattttag tagagttgca caatagcccc ccacccgttc tgcacgggtt
    79981 aatgaggaac tttgccctcc cagccaggcg atccgctgat gcccattgcg aatgagatgc
    80041 tccgtcaaca actgtgcagc ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga
    80101 taactggcac gggaagcgaa aatcaccggg atagcttttt cttctgccat ccgtcgcagg
    80161 tcatcgctgc ttcctgcagc cccggcaatt accacaccat cgacaccctg attcagtaac
    80221 agtgaaaacc gctgtgccag ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc
    80281 cgtccctgcg cttccagagc ttccgtcaat ccggccgtca attcggcgta aaacggcgca
    80341 gataaatcac ggacgatcaa accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc
    80401 tggcgattgc gcacaaatcc cagctcttca atggcggcgt taacgcgttc tcctgtggcg
    80461 gtagagattc gccctttgcc actaagcacc agcgaaacgg tacttaccga cacgcccgca
    80521 gccagcgcaa catcatgaat ggttattttt ttggcggtag ccatgcagat gacctactcc
    80581 ctgattttgt gacagataaa acgttttacc ttttatttat cttatacccg ctattatcgt
    80641 tgcgtaatgt gatttatgcc tcactaaaat ttgataaaac gttttatctt ctcgcgcaat
    80701 ttactgaatc cagattgttc tctacgagga gtcgttttat gacggcgaaa acagcaccga
    80761 aagtcacgct gtgggagttc ttccagcagt taggcaaaac cttcatgtta cccgtggcat
    80821 tattgtcgtt ctgcggcatt atgctcggca ttggtagttc ccttagtagc cacgatgtca
    80881 tcaccctgat cccggtcttg ggcaatcccg tgttgcaggc tatctttacc tggatgagta
    80941 agattggctc gtttgctttt agtttcctgc cagtcatgtt ctgtattgcc atcccgctgg
    81001 gcctggcacg cgaaaacaaa ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa
    81061 tgaacctcgc ggtaaacttc tggttgacca ataaaggcat tctgccaacc acggatgccg
    81121 ccgttctgaa agccaataac atccagagca ttcttgggat ccagtcgatc gacaccggga
    81181 tcctcggtgc ggtgatcgcc ggtattatcg tctggatgct gcatgagcga ttccataata
    81241 tccgcctgcc ggatgcgctg gcattcttcg gcggtacgcg cttcgtacca atcatctcct
    81301 cgctggtgat gggcattgtc ggtctggtga ttccattagt ctggccaatt ttcgccatgg
    81361 gtattagcgg cttaggccat atgatcaata gcgcgggtga tttcggaccg atgctgtttg
    81421 gtaccggtga acgtctgctg ttgccgtttg gtctgcatca cattctggtg gcattaattc
    81481 gctttaccga cgcaggcggc acgcaggaag tctgcggtca aaccgtcagc ggtgcactga
    81541 ccatcttcca ggcgcaattg agttgcccga ccactcacgg tttttctgaa agcgccacgc
    81601 gtttcctttc gcaaggcaaa atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag
    81661 ctatgtatca ctgcgcgcgc ccggaaaatc gccataaaat taaagggctg ctgatttctg
    81721 gcctgatcgc ctgtgtcgtt ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg
    81781 tagcgccagt tctgtatgtc atccacgcgc tgttaaccgg cctcggcttc accgtcatgt
    81841 ctgtgctcgg cgtcaccatc ggtaataccg acggcaatat catcgacttc gtggtgttcg
    81901 gtattttgca tggtctgtca accaagtggt acatggtgcc agtggtggcg gcaatctggt
    81961 ttgtcgttta ctacgtcatc ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg
    82021 ggcgcgatag cgaagtggcc agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat
    82081 caggttacaa cgttccggca atcctcgaag ccttaggcgg tgccgacaat attgtcagcc
    82141 ttgataactg cattacccgt ttgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc
    82201 aggcactgaa ggacaatcgg gcaattggcg tagtacaact taatcaacat aacctgcagg
    82261 ttgttatcgg gccacaagtt cagtcagtaa aagatgaaat ggccggtctg atgcatactg
    82321 tccaggcata aggataagat atgttcgatt tttcaaaggt cgtggatcgt catggcacat
    82381 ggtgtacgca gtgggattat gtcgctgacc gtttcggcac tgctgacctg ttacctttca
    82441 cgatttcaga catggatttt gccactgccc cctgcattat cgaggcgctg aatcagcgcc
    82501 tgatgcacgg cgtgtttggc tacagccgct ggaagaacga tgagtttctc gcggctattg
    82561 cccactggtt ttccacccag cattacaccg ccatcgatcc tcagtcggtg gtgtatggcc
    82621 cttctgttat ctatatggtc tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg
    82681 tggtgatcca cacacccgcc tatgacgcat tttacaaggc cattgaaggt aatcagcgca
    82741 cagtaatgcc cgttgcttta gagaagcagg ctgacggctg gttttgcgat atgggcaagc
    82801 tggaagccgt gttggcgaaa ccagaatgta aaattatgct cctgtgtagc ccacagaatc
    82861 ctaccggaaa agtgtggacg tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc
    82921 atggtgtgag ggttatttcc gatgaaatcc atatggatat ggtttggggc gagcagccgc
    82981 atattccctg gagtaatgtg gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa
    83041 gtttcaatat tcccgcgctg accggtgctt acgggattat agaaaatagc agcagccgtg
    83101 atgcctattt atcggcactg aaaggacgtg atgggctttc ttccccttcg gtactggcgt
    83161 taactgccca tatcgccgcc tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct
    83221 atctgaaaga taacctgacg tatatcgcag ataaaatgaa cgccacgttt cctgaactca
    83281 actggcagat cccgcaatcc acttatctgg cctggcttga tttacgtccg ttgaatattg
    83341 acgacaacgc cttgcaaaaa gcacttatcg aacaagaaaa agtcgcgatc atgccggggt
    83401 atacctacgg tgaagaaggt cgtggttttg tccgactcaa tgccggctgc ccacgttcga
    83461 aactggaaaa aggtgtggct ggattaatta acgccatccg cgctgttcgc taaccccaat
    83521 tgcgcaacgt aaaaaatcgt tgcgcaatcg tggattttta ccctgctttg tttttataat
    83581 ggtgcgcact tttatatcca gaaaaagagt gcgaccatga ttgataccac cctgccatta
    83641 actgatattc atcgccacct tgatggcaac attcgttccc agaccattct tgaacttggc
    83701 cgccagtata atatctcgct tcctgcacaa tccctggaaa cactgattcc ccacgttcag
    83761 gtcattgcca acgaacccga tctggtgagc tttctgacca aacttgactg gggcgttaaa
    83821 gttctcgcct ctcttgatgc ttgtcgccgc gtggcatttg aaaacattga agatgcagcc
    83881 cgtcacggcc tgcactatgt cgagctgcgt ttttctccag gctacatggc aatggcacat
    83941 cagctgcctg ttgcaggtgt tgttgaagcg gtgatcgatg gcgtacgtga aggttgccgc
    84001 acctttggtg tgcaggcgaa gcttatcggc attatgagcc gaaccttcgg cgaagccgcc
    84061 tgtcagcaag agctggaggc ctttttagcc caccgtgacc agattaccgc acttgattta
    84121 gccggtgatg aacttggttt cccgggaagt ctgtttcttt ctcacttcaa ccgcgcgcgt
    84181 gatgcgggct ggcatattac cgtccatgca ggcgaagctg ccgggccgga aagcatctgg
    84241 caggcgattc gtgaactggg tgcggagcgt attggacatg gcgtaaaagc cattgaagat
    84301 cgggcgctga tggattttct cgccgagcaa caaattggta ttgaatcctg tctgacctcc
    84361 aatattcaga ccagcaccgt ggctgagctg gctgcacatc cgctgaaaac gttccttgat
    84421 catggcattc gtgccagcat taacactgac gatcccggcg tacagggagt ggatatcatt
    84481 cacgaatata acgttgccgc gccagctgct gggttatccc gcgagcaaat ccgccaggca
    84541 cagattaatg gtctggaaat ggctttcctc agcgctgagg aaaaacgcgc actgcgagaa
    84601 aaagtcgcag cgaagtaaca aaaatggatg gtgcaaattg caccatccat tttcacgcaa
    84661 ggcacaaagt cgcgcgatgt ttggcggatt cgatgcccag ctcaatcaac tccattacct
    84721 ggattgcctg gcttgccgga accggatttt caccatcgcc atttaacgca tcacgaatag
    84781 ccgcatagta agccggataa ttcccaggca ccgtcaacag cgtttcttcg acacgttcct
    84841 caccttccac gcgggtaagt acgccatcac gcatatcgta gccccagtct tcctgcggca
    84901 gacgctcgcc acttttcaga cgttcttcct gtggatcgag gccatatttc acataactgc
    84961 ctcgggatcc atgcacgata taccgtgctg actcagcggc tgccagcatg gtaccgtgta
    85021 aaatgactcg ccgctgtgga taggacaaga tggcgtggaa ataatcggtc gactgcgctc
    85081 caggccgtaa ctgtgccaag tccaccgtca tgctgaccgg taaaccaaat aatgtaatag
    85141 cctgatcaag aagatgcggt gctaaatcgt accagatacc actgcctggg ccaccctgtt
    85201 cacgccaacg atcgcgcacc tgcggacgga agcggtcaaa atgagactca aagtaagcga
    85261 cttcacccag cacgccttcc gcgagtaaac cttttagcgt caagaaatcg ctatcccagc
    85321 gacggttatg gaatacagac agcacacgcc ccaggctttt tgccagcgca tccagctctc
    85381 gcgcttgtga cagtgtcacg gtaaagggtt tatcaacgac cacatgttta cccgcctcaa
    85441 gcgctgcttt ggctaacggg aaatgggtat cgttgggtgt aggaatgaca atcaggtcta
    85501 tgttgggatc gttaaacaga tgcttcggct cagagacaac cgtaaccgtt ggccagtcgg
    85561 cttttacttt tgtttcatca ctgctggaga ttaccgccag ttccagcccg ggcgtgcccg
    85621 caatcagggg cgcatggaag gttttgctcg cataaccata cccaatcaac ccaacacgga
    85681 tgttgtcgct catgtcattg cctctcattg aagtatgatg gctatttgac actatccttt
    85741 accctcgctc aacagtttaa taacctgcca gcaataaggg atgttgttta acttaagtca
    85801 aaaaaatagc gatttttcca acgacaaaag ctaaatatcg caaaaacctc agtaaaaatc
    85861 ttgctggagc tattattgct aagtaacatt taccccctga agttaatgga tcaatcaaca
    85921 gagatgtgag ttgtaatgaa tcgtcttatt gaattaacag gttggatcgt tcttgtcgtt
    85981 tcagtcattc ttcttggcgt ggcgagtcac attgacaact atcagccacc tgaacagagt
    86041 actacggtac aacacaagta agctctgcgc ttgtagagcg acatgctgcc cgtcagggtg
    86101 catgttttca cttgtcggat attaaaccag gaatttatta tcttgttcgg tgttgttggt
    86161 gattgtcagg gatagtaaag cgcgttactc ttttcatagc agtttttcat ccctgaatat
    86221 ttgccatcag cgcattctta ttattgtaat aacacaagcg ttcacttttg gtgaaatttt
    86281 atcatcacta ataataccta cccctttggt attaattatt ttatggacct tttatgactg
    86341 ttcaggacta cttattaaaa tttcgcaaaa tcagttcact cgaaagtctg gaaaaacttt
    86401 acgaccatct taattacacc ctgacggacg atcaggaact gatcaatatg tatcgtgctg
    86461 ccgatcaccg tcgcgcagag ctggtttctg gcgggcgttt gtttgacctc ggccaggtac
    86521 cgaagtccgt ctggcactat gtccaataaa gaaagtagcg atcggttata aaagccttat
    86581 aataacgccc ctaaaattag atttgcctgt ttgcgtcgct attctggaga acctatgacc
    86641 accacaacgc cacaacgtat tggaggctgg ttgcttggcc cgttggcctg gcttttagtc
    86701 gctctgttaa gtacgacgct ggcgttgttg ctgtataccg ctgcgttatc ttctccgcaa
    86761 acatttcaaa cgctcggcgc acaagcactg acgacacaaa tcttatgggg cgtttctttt
    86821 attactgcta tcgccatgtg gtattacaca ctgtggttga ccatcgcttt ctttaaacgt
    86881 cggcgttgtg ttcctaaaca ctatattatc tggctgctta tttccgtatt gctggcagta
    86941 aaagcgttcg ctttttcacc cgtggaagat ggcatagccg ttcgccagtt gctgtttact
    87001 ttgttggcaa ccgcattgat cgtcccctat ttcaaacgct cgtcgcgggt gaaagcgacg
    87061 tttgtgaatc cgtaataacc ttacagttaa cctgttgtcg cctgctctgg attaacggat
    87121 aataggcggc ttttttattt caggccgaaa aatgactgac tacctgttac tgtttgttgg
    87181 aactgtactg gtcaataact ttgtactggt caagtttctc ggtctctgtc cgtttatggg
    87241 agtttccaaa aagctggaaa ccgcgatggg catggggctg gcaacaacgt ttgtgatgac
    87301 gctggcgtct atttgcgcct ggcttatcga tacgtggatt ttgatcccac ttaatctgat
    87361 ttacctgcgc accctggcat ttattctggt gattgctgtg gtcgtgcagt tcaccgagat
    87421 ggtggtgcgc aaaaccagcc cggtgcttta ccgtttgctg gggatttttt tgccgcttat
    87481 caccaccaac tgtgccgtgc tcggcgtggc gttgctgaat atcaatctcg ggcacaattt
    87541 cttgcagtcg gcgctgtacg gtttttccgc cgctgtcggt ttttcgctgg tgatggttct
    87601 cttcgcagcc atccgcgaac gccttgctgt ggctgatgtc ccggcacctt ttcgcggtaa
    87661 tgccattgcg ttaattaccg caggtcttat gtctctggcc tttatgggct ttagtggttt
    87721 ggtgaagttg taatgaatgc tatctggatt gccgttgccg ccgtgagcct gctgggcctg
    87781 gcgtttggcg ccattctggg ttatgcctcc cgccgttttg cggtggaaga cgatccggtc
    87841 gttgagaaaa ttgacgaaat cttaccgcag agccagtgtg gtcagtgcgg ttatcccggc
    87901 tgtcgcccct acgcggaagc catcagctgt aacggtgaaa aaatcaaccg ttgcgcccca
    87961 ggtggcgaag ctgtgatgct aaaaattgcc gagttgctta atgtcgagcc gcagccgctg
    88021 gatggcgaag cgcaagagct aacgcctgcg cggatggtgg cggttattga tgaaaataac
    88081 tgtattggct gcactaaatg tattcaggcg tgtccggtag acgccatcgt tggcgctacc
    88141 cgtgccatgc atacggtaat gagtgatctc tgtacgggct gcaatttatg tgttgatccg
    88201 tgcccgacgc actgcatctc gttgcaaccg gtcgcagaaa cacctgactc ctggaaatgg
    88261 gatctgaaca ccattcccgt gcgtatcatt cccgtggaac accatgctta agttattctc
    88321 tgcattcaga aaaaataaaa tctgggattt caacggcggc atccatccac cggagatgaa
    88381 aacccagtcc aacggtacac ccctgcgcca ggtacccctg gcgcagcgtt ttgtcattcc
    88441 actgaaacag catattggcg ctgaaggtga gttgtgcgtt agcgtcggcg ataaagtatt
    88501 gcgcggccag ccgcttaccc gtggtcgcgg caaaatgctg cctgttcacg cgcccacctc
    88561 gggtaccgtt acggctattg cgccccactc tacggctcat ccttcagctt tagctgaatt
    88621 aagcgtgatt attgatgccg atggtgaaga ctgctggatc ccacgagacg gctgggccga
    88681 ttatcgctct cgcagtcgcg aagagttaat cgagcgcatt catcagtttg gtgttgccgg
    88741 gctgggtggt gcgggattcc cgacaggcgt taaattgcag ggcggcggag ataagattga
    88801 aacgttgatt atcaacgctg ctgagtgcga gccgtacatt accgccgatg accgtttgat
    88861 gcaggattgc gcggctcagg tcgtagaggg tattcgcatt cttgcgcata ttctgcagcc
    88921 acgcgaaatt cttatcggca ttgaagataa caaaccgcag gcgatttcca tgctgcgcgc
    88981 ggtgctggcg gactctcacg atatttctct gcgggtgatt ccaaccaaat atccttctgg
    89041 cggtgctaaa caattaacct acattctgac cgggaagcag gttccacatg gcgggcgttc
    89101 atctgatatc ggcgtattaa tgcaaaacgt cggcactgct tatgcagtga aacgtgccgt
    89161 tattgatggc gagccgatta ccgagcgtgt cgtaaccctg actggcgaag caatcgctcg
    89221 cccgggcaac gtctgggcac ggctggggac gccagtgcgt catttattga atgacgccgg
    89281 attctgcccc tctgccgatc aaatggtgat tatgggcggc ccgctaatgg gctttacctt
    89341 gccatggctg gatgtcccgg tcgtaaagat taccaactgt ctgttggctc cctctgccaa
    89401 tgagcttggc gaaccacagg aagaacaaag ctgcatccgg tgtagcgcct gtgctgacgc
    89461 ctgccctgcg gatcttttgc cgcaacagtt gtactggttc agcaaaggtc agcaacacga
    89521 taaagctacc acgcataaca ttgccgactg cattgaatgt ggtgcttgcg cgtgggtttg
    89581 cccgagcaat attcccctgg tgcaatattt ccgtcaggaa aaagctgaaa ttgcggctat
    89641 tcgtcaggaa gaaaagcgcg ccgcagaagc caaagcgcgt ttcgaagcgc gccaggctcg
    89701 tctggagcgc gaaaaagcgg ctcgccttga acgacataag agcgcagccg ttcaacctgc
    89761 agccaaagat aaagatgcga ttgctgccgc tctggcgcgg gtgaaagaga aacaggccca
    89821 ggctacacag cctattgtga ttaaagcggg cgaacgcccg gataacagtg caattattgc
    89881 agcacgggaa gcccgtaaag cgcaagccag agcgaaacag gcagaactgc agcaaactaa
    89941 cgacgcagca accgttactg atccacgtaa aactgccgtc gaagcagcta tctcccgcgc
    90001 caaagcgcgc aagctggaac agcaacaggc aaatgcggaa ccagaacaac aggtcgatcc
    90061 gcgcaaagct gccgtcgaag ccgctattgc ccgtgccaaa gcacgcaagc tggaacagca
    90121 acaggctaat gcggaaccag aagaaccggt cgatccgcgc aaagccgccg tcgaagccgc
    90181 tattgcccgc gccaaagcac gcaagctgga acagcaaccg gctaatgcgg agccagaaga
    90241 acaggttgat ccgcgcaaag ctgccgtcga agcggctatt gcacgcgcca aagcacgcaa
    90301 gctggaacag caacaggcta atgcggtacc agaagaacag gttgatccgc gcaaagcggc
    90361 agttgccgcg gctattgccc gcgttcaggc caaaaaagcc gcccagcaaa aggttgtaaa
    90421 cgaggactaa atggtattca gaatagctag ctccccttat acccataacc agcgccagac
    90481 atcgcgcatt atgctgttag tgttgctcgc agccgtgcca ggaatcgcag cgcaactgtg
    90541 gttttttggt tggggtactc tcgttcagat cctgttggca tcggttagtg ctctgttagc
    90601 cgaagctctc gtactcaaac tacgcaagca gtcggtagcc gccacgttga aagataactc
    90661 agcattgctg acaggcttat tgctggcggt aagtattccc cccctcgcgc catggtggat
    90721 ggttgtgctg ggtacggtgt ttgcggttat catcgctaaa cagttgtatg gcggtctggg
    90781 gcaaaacccg tttaatccgg caatgattgg ttatgtggtc ttactgatct ccttcccggt
    90841 gcagatgacc agctggttac cgccacatga aattgcggtc aacatccctg gttttatcga
    90901 cgccatacag gttattttca gcgggcatac cgccagtggt ggtgatatga acacactacg
    90961 cttaggtatt gatggcatta gtcaggcgac accgctggat acatttaaaa cctctgtccg
    91021 tgccggtcat tcggttgaac agattatgca atatccgatc tacagcggta tgctggcggg
    91081 cgctggttgg caatgggtaa atctcgcctg gctggctggc ggcgtgtggt tgctatggca
    91141 gaaagctatt cgctggcata ttcccctcag cttcttagta acgctgacgt tatgcgcaac
    91201 gttgggctgg ttgttctcac cagaaacact ggccgcaccg caaattcatc tgctgtctgg
    91261 agcgaccatg ctcggcgcat tctttatttt gactgacccg gttaccgctt ctacgaccaa
    91321 tcgtggtcgt cttattttcg gcgcgcttgc gggcttatta gtctggttga tccgcagttt
    91381 cggcggctat cctgacggcg tggcttttgc cgtcctgctg gcgaacatca cggttcctct
    91441 gatcgattac tacacccgtc cgcgcgtcta cggccatcgc aaagggtaaa ccatgctgaa
    91501 aactatccga aaacacggca ttacgttggc gctatttgca gcgggttcaa cagggttaac
    91561 tgcggccatc aaccagatga ccaaaacgac gattgctgaa caggccagtc tgcaacaaaa
    91621 ggcgttattt gatcaggtgc tgccagccga acgctataac aatgcgctgg cacagagttg
    91681 ctatctggta actgcgccag agttaggtaa aggtgagcat cgggtttaca tcgccaaaca
    91741 ggatgacaaa ccggtagccg ccgttctgga agcaaccgcg ccagatggct attccggtgc
    91801 gattcagctg ctggtgggag ccgattttaa cggcacggta cttggcacgc gcgtgacaga
    91861 gcaccacgaa acgccagggc ttggcgataa aatcgaactg cgcctttctg actggatcac
    91921 ccattttgcg ggtaaaaaaa tcagtggtgc ggatgatgcg cactgggcgg tgaagaaaga
    91981 tggtggtgat ttcgaccagt tcaccggcgc gacgattact ccccgcgcgg tggttaatgc
    92041 ggtaaaacgc gccggattgt acgctcagac gttaccggca caactttctc aacttcctgc
    92101 ctgtggagaa taaagcgtga gcgaaattaa agacgttatt gttcaggggt tgtggaaaaa
    92161 caactctgcg ctggtccagt tgctcggcct ttgtcctctg ttggcggtca cgtccactgc
    92221 cactaacgct ctgggtttag gacttgcgac tacgctggta ctgacgctga ccaacctgac
    92281 catttcgacg ctgcgtcact ggacgccagc cgagatccgc attcccattt acgtgatgat
    92341 catcgcctcg gtggtcagcg ctgtacagat gctgatcaac gcctacgctt ttggcctgta
    92401 tcaatcatta gggattttta ttccgctgat tgtcactaac tgtatcgttg tgggccgcgc
    92461 tgaagccttc gccgccaaaa aaggtccggc gctttcggca ctggacggct tttcaattgg
    92521 tatgggcgca acctgcgcca tgttcgtgct gggttcacta cgcgaaatta tcggcaatgg
    92581 cacgttgttt gacggtgcag attcgctgtt aggtagctgg gcaaaagtat tacgcgtgga
    92641 gattttccac accgactccc ctttcctgct ggcgatgctg ccaccaggtg catttattgg
    92701 cctgggactg atgctggcag gaaaatacct gattgatgaa agaatgaaaa agcgccgtac
    92761 tgaagcagct gccgaacgtg cattgccaaa cggtgaaaca gggaatgtct gatgaacaaa
    92821 gcaaaacgcc tggagatcct cactcgcctg cgtgagaaca atcctcatcc caccaccgag
    92881 cttaatttca gttcgccttt tgaattgctg attgccgtac tgctttccgc tcaggcaacc
    92941 gatgtcagtg ttaataaggc gacggcgaaa ctctacccgg tggcgaatac gcctgcagcg
    93001 atgcttgaac tgggcgttga aggggtgaaa acctatatca aaacgattgg gctttataac
    93061 agcaaagcag aaaatatcat caaaacctgc cgtatcttac tggagcagca taatggcgag
    93121 gttccggaag atcgtgctgc gcttgaagcc ctgcccggcg taggtcgtaa aacagccaac
    93181 gtcgtattaa acactgcatt cggctggccg actattgctg tcgacacgca cattttccgc
    93241 gtttgtaatc gtactcaatt tgcgccgggg aaaaacgtcg aacaggtaga agaaaagcta
    93301 ctgaaagtgg ttccggcaga gtttaaagtc gactgccacc attggttgat cctgcacggg
    93361 cgttatacct gcattgcccg caagccccgc tgtggctctt gtattattga agatctttgt
    93421 gaatacaaag agaaagttga catctgaaga aaaggggtaa caccgattac cccattgata
    93481 acctttcttt atcctctttt aaaacatctt tttaacgtca atgatgccat tgcttagcgt
    93541 tatcatcagg taatccgttt gcggataacc aaaaatgcag gttaattgtt tttttaatag
    93601 cgaaattttc tattcattcg tgatcaagat cacgatgctg tggcaatgta aaaacattgc
    93661 aagaatcagg ttaaaatcat ttaaatttac acacgcaaca aatattgacc tacaaaacat
    93721 tacactggct atttttcaga aactggacta tctcactagg aaaaacgcta aatagcagaa
    93781 catatcgccg tcaagccatc atccccctct caatgcagtg aaaaaatctg ccgttacgtt
    93841 ttttgaaaaa tttaacgctg gataacattt cccggaatgg ttgaattccc cgcctcagtt
    93901 atatgtaaca gattattaca aaggacttgt ctgaaagtgc aagatagtga acattacctg
    93961 ccgtttcccc tcccactata acaattgcgc gtatgttttt aggcataacg cgagaaagca
    94021 cccccgttaa tatgggatgt aaaaaaagag gtaaaagtgt ccactgcaaa ccaaaaacca
    94081 actgaaagcg tcagtttgaa cgctttcaaa caaccgaagg cgttctatct catcttctcg
    94141 attgagttat gggaacgttt tggttattac ggcctacaag gaattatggc tgtttacctg
    94201 gttaaacaac tgggtatgtc tgaagcggat tcaatcaccc ttttctcttc ctttagtgcc
    94261 ctggtttatg gtctggtcgc tatcggcggc tggttaggtg acaaggtact gggtactaaa
    94321 cgcgtaatta tgctcggcgc tattgtgctg gcgattggtt atgctctggt tgcctggtct
    94381 ggtcacgacg ccggtatcgt ttatatgggt atggcggcta ttgcggtcgg taacggcctg
    94441 tttaaagcta acccgtcttc tctgctttct acatgctatg agaaaaacga cccgcgtctg
    94501 gacggtgcat tcaccatgta ctacatgtcc gtcaacatcg gctctttctt ctctatgatc
    94561 gctaccccgt ggctggccgc gaaatacggc tggagtgttg cgtttgcgtt gagcgttgta
    94621 ggcctgctga tcactattgt taacttcgcc ttctgccaac gctgggttaa acagtacggt
    94681 tcaaaaccag acttcgagcc tatcaactac cgtaacctgc tgctgaccat tattggtgtt
    94741 gtggcactga tcgctatcgc cacctggctg ctgcacaatc aggaagttgc gcgtatggcg
    94801 ctgggcgttg ttgcctttgg tatcgtggtt atcttcggta aagaagcctt cgcgatgaaa
    94861 ggtgctgcgc gtcgtaaaat gatcgttgcc ttcatcctga tgctcgaagc cattatcttc
    94921 ttcgtgctgt acagccagat gccaacgtca ctgaacttct ttgcgattcg taacgttgaa
    94981 cacaccattc tgggcctggc cgtagaacct gagcagtatc aggcactgaa cccgttctgg
    95041 atcatcattg gtagcccgat tctggccgct atctataaca agatgggcga taccctgccg
    95101 atgccaacta agtttgcaat cggtatggtg atgtgttctg gtgcgttcct gattctgccg
    95161 ctgggtgcaa aattcgcgtc tgacgcaggt atcgtgtctg taagctggct ggtcgcaagc
    95221 tatggcctgc agagtatcgg ggaacttatg atctctggtc tgggtctggc aatggttgct
    95281 caactcgttc cgcagcgtct gatgggcttc attatgggta gctggttcct gaccactgcc
    95341 ggtgcaaacc tgattggtgg ttatgttgcg ggtatgatgg ctgtgccgga taacgttacc
    95401 gatccgctga tgtcactgga agtctatggt cgcgtattct tgcagattgg tgtcgctact
    95461 gccgttattg cagtactgat gctgtttacc gcgccgaaac tgcaccgcat gacgcaggat
    95521 gacgctgctg acaaagcggc gaaagctgct gtagcgtaaa attcagggaa actcttttac
    95581 aagccgctaa cttttcgtta gcggcttttt ttttgttcag caacgcacta ccatacttta
    95641 aaccacagac aaaaggagtt accgatgaaa ttgttctaca aaccgggcgc ctgctctctc
    95701 gcttcccata tcaccctgcg tgagagcgga aaggatttta cgctcgtcag tgtggattta
    95761 atgaaaaaac gtctcgaaaa cggtgacgat tactttgccg ttaaccctaa gggacaggta
    95821 cctgcattgc tgctggatga cggtactttg ctgacggaag gtgtagcgat tatgcagtat
    95881 cttgccgaca gcgtccccga ccgccagttg ctggcaccgg taaacagtat ttcccgctat
    95941 aaaaccatcg aatggctgaa ttacatcgcc accgagctgc ataaaggttt cacacctctg
    96001 tttcgccctg atacaccgga agagtacaaa ccgacagttc gcgcgcagct ggagaagaag
    96061 ctgcaatatg tgaacgaggc actgaaggat gagcactgga tctgcgggca aagatttaca
    96121 attgctgatg cctatctgtt tacggtactg cgctgggcat acgcggtgaa actgaatctg
    96181 gaagggttag agcacattgc ggcatttatg caacgtatgg ctgaacgtcc ggaagtacaa
    96241 gacgcgctgt cagcggaagg cttaaagtaa tagccgtcgg cccacagttc gttgtgggcc
    96301 gaaatgagat atttcagagc tttgttgcgc tgaagtaatg ttctggtttg gcaatacgat
    96361 cctgagcagc aaccacttgc aactcatatt cctgcattgc tttggtggtc accatgattt
    96421 cgtatactgc agcggtcaca tgttccaggg cctcctgcag cgttgccccc tgaagcagtt
    96481 tcaccagcag taaaccgctc gtcacatcac caacacctac cggctggcgc ataccaaaat
    96541 ccaccagtgg acggctgata tgccaggctt catcggcggt gaccagcagc atttcaaaac
    96601 ggtcacggct gtagccagct cgcgccaggt gtttaaccaa cacaatttgt ggcccttgcg
    96661 caatgagttc gcgcgctgcc agaaccgctt cttcgacgtt atttaccgca tgctcacaga
    96721 gtatttccag ctcaacaaga ttcggcgcaa tgatatcgct ggcaggcaaa ccgtgccgca
    96781 catgaaactc tgcgacaccc ggtgcaacga tacagccttt ttccggatga cccattaccg
    96841 gatcgcaaaa atatttcgcc tgcggattcg cggctttcac ctgacggacg ataccgagga
    96901 tatgttcacc ctgctccgcc gatcccagat agccacttaa tacggcatca caggtgtgta
    96961 atttatcaat ggcggcaatg ccttgcacaa tttcggttaa atggctgggc ggcatcacgc
    97021 agccagtcca tttgccgtat tgggtgtgat tagaaaattg aacggtgttc agcggccaga
    97081 cgttcgcgcc caggcggcgc atcggaaact ctgccgcact gttacccgca tgaccataaa
    97141 caacgtgaga ctggatagcg agaatatttt tcatcatgtt ccctgtataa aaaccagggg
    97201 gagtgatttc tcactccccc tttccactta atgcattatt tccagcaaat cagacagtaa
    97261 ttctttttgc cgcgacgcaa taaggtaaaa cgaccaaaca gacgatcttc ttctttaaag
    97321 aagtattcag gatcggactg tttttcaccg ttaatggtga tggcattgga ggcgatagtt
    97381 ttacgcgcct gaccacggga aggttgcagt tcagaatcga ccagcgcctg catcaggtct
    97441 gcgccctttt ccatctcaac catcggtacg ccgtcctgcg ccagctgttc gaagtccgct
    97501 tcactcagcg cactcaagga accgctgaac aggcattcgg taatacgttt tgccgcctgc
    97561 aaaccttctt caccgtgaac cagacgggtc acctgctccg ccagtacata ctgggcgcgc
    97621 ggtgctttac cgctgttttt atcttcttct tccagggcgt tgatctcttc aatgctcata
    97681 aaggtgaaga acttcaggaa gcggtaaacg tcggcatccg cagtgttgat ccagaactgg
    97741 tagaatttgt acgggctggt tttcttcgga tccaaccaga ctgcgccgcc ttcagtttta
    97801 ccaaatttgg tgccatctgc tttggtgatc agcggaacgg tcaggccaaa cacctgattc
    97861 tgatgcagac gacgggtcag gtcgatacca gaagtgatgt taccccactg gtcagaacca
    97921 ccaatttgca gcaccacacc gtactgtttg ttcagacagg cgaagtcata accctgcaac
    97981 aggttgtagg aaaactcagt gaacgaaatc ccctgatctt cacggttgag acgctgctta
    98041 accgcttctt tgttgatcat ctggttaacg gagaagtgtt tgccaatatc gcgcaggaag
    98101 gtcagcacat tcatattgcc gaaccagtca tagttgttcg ccgcgatagc agagttttct
    98161 ccacagtcga aatcgaggaa cggggcaacc tgcttgcgga ttttgtccac ccactcctga
    98221 acagtttctt cggtgttcag cttacgctcg gcagctttga agctcgggtc gccaatcaga
    98281 cccgtcgcgc cgcctaccag cgcaaccggc ttgtggcccg cctgctggaa gcgtttcagg
    98341 cataacaatg gaacaagatg ccccaaatgc aagctgtcag cggtaggatc gaagccgcaa
    98401 tagagcgcga tcgggccttg cgccagtcgc tctgctaacg cttcctcgtc cgtcacctgg
    98461 gctaccagcc cccgctcttg caattgttta atcaagttac tgcttgccat caaaatctcc
    98521 atgtatataa cgactgcacc tttgccggta cacgactttt cgccagatgc gaaagagaca
    98581 tagaataaag tgccagaatc aggagtacca gcgattaaag caagattttt gcatcttttc
    98641 agggtgcaag acgatcaatc ttccacgcat cattttcacg ctggtacaaa aagcggtcat
    98701 gcaggcgatg ctcaccgccc tgccagaact caatctgttc aaggctgacg cgaaaaccgc
    98761 cccaaaagct cggcaatggc acttcgccct gttgaaactt ctgcttcagc tccaggaatt
    98821 tactttcaag gataccgcgg gcagaaatgc gactggactg cttcgaaacc catgcaccaa
    98881 tctggctatc acgcgggcgg ctatgaaaat atttcatcac ttcgagagtc gaaagtcgtt
    98941 ctgctttacc gatcaccatc acctggcgtt caagggtatg ccacgggaac agcaggctaa
    99001 cgcgcggatt attttcaatt tgatgtgctt tacggctacc gaggttggtg taaaacacca
    99061 tgcctttttc gtcgtaatgt ttgagtaaaa cgatgcgctg ataaggctga ccatgttcat
    99121 ccacggtagc gaccaccatc gcggtagggt ccgccagttt ggcttcacaa gcctgagaga
    99181 gccaacgttc aaaaagggtt aatggatcgg cgggaagatc gcggcggcgt aacccgcctt
    99241 tggtgtattc acggcgcaga tgcgcgattt gctgcaattc gtcgttatca gacatggttt
    99301 tctttacgga ttgtcagtgg gtgacgctat tgtgcgccgc ccctggaaaa atctcaacgc
    99361 cgtgaatttt gtaactgaca gttattcaag acgatgcggt cgcgtttata gacagtcgct
    99421 tcctcgcctt tcgaccagaa aacatagatt ccgtcagtgt aacgtgcacc agaggctgaa
    99481 atgccctgtt tgagatgcag tagttgatta tcgtaaacaa aactgacttc ctggcgcgga
    99541 ttattcagtt tgaccgtcaa cggtttttca tcacactggt attccagcgt atcggtttgc
    99601 atgcgctcaa ccagttgatt gaacgcgcta cagccggtga gaagcaccgg caaacagatc
    99661 agtaacagtt ttttcataga catttcccga agactttcct ggtctggagg gcaatacgcc
    99721 ctccctaacg ttccaagtgt aacggcagac gcggtaagaa aaattcagtt aactctgata
    99781 tcacgggtta gccgggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga
    99841 aggcagattt cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc
    99901 ttccatgtca tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctggcag
    99961 taatgccgcc agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa
   100021 tcgttcgcag ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc
   100081 cagtgtggcc tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag
   100141 ccagccgtag ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatatgg
   100201 gtcgctgagc atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc
   100261 atctttatcg taaggtttac cggcctgacg ccagatccag gcatccatca gcatgttacc
   100321 aggaccggta tcgtagccgc caaccggctg cccaggaatg agcagtgaca gattggcgat
   100381 gccgccaata ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa
   100441 cgcaggtacc agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc
   100501 gacaaccgta attccggtgc gtgccacaat ttgattgtta tcgccaatct gtaaagtgtg
   100561 tggtgccacg cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat
   100621 atctctcgcc tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg
   100681 cccgagttga gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat
   100741 atccagtaca gcctgtttca gagacaccgg gatcggccag ctcaaacttg ccagctgtgc
   100801 gacccggtgt tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga
   100861 cataacgcca ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg
   100921 caccactcaa acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg
   100981 tagtaaaaca ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga
   101041 tcctactttc attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat
   101101 gccatatact ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt
   101161 tcaatgatta aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt
   101221 aataacgaca ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat
   101281 gtcagctatg gaaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc
   101341 aacgttatcg gtgcaattgg cggtgctgtt cttggtggtt tcctgggaaa tactgttggt
   101401 ggcggaaccg ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt
   101461 cagggcgtac agagtgcaat gaacaaaacg caaggtgtcg agctggaaat tcgtaaagac
   101521 gatggtaata ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa
   101581 cgtgtcgtac tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg
   101641 cgtgtggtca ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa
   101701 tgatattatg ctcaagtttt gcgatgagcg taatcagttg ttccagttcc tctgcggaga
   101761 tgccatgtaa gatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagcg
   101821 gctctgcctt ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac
   101881 gcgaaattaa ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga
   101941 tgccaatcgc ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt
   102001 gtaacgtaac ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag
   102061 cacgccatat gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct
   102121 ccttataatt agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat
   102181 tgctaaaaca aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg
   102241 cgtaaatttt tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa
   102301 ggaatctact ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg
   102361 ttcggtgcgt ccgtctactt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc
   102421 tggcttgtcg tacaccgcct gcttcgtggc tggatctacg ccggtgacat ctggcatccc
   102481 ttgttaatgg atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt
   102541 gcgtggtaac tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcggtag
   102601 ttgcggttct tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg
   102661 acgggaaaat acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg
   102721 agctgaatat aaaagacaac cagctggtta acgcggggga tcttctgctc acgatcgata
   102781 aaacgccctt tcagatcgcc gagctgaacg cacaggcaca gctggcgaaa gcgcaatctg
   102841 acctcgcaaa agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt
   102901 ctgccgaaga actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttgatg
   102961 ccgcacaggc gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc
   103021 cggtgagtgg atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga
   103081 aaccgctgtt tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa
   103141 ccaagttgcg ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca
   103201 aaacgttaca gggtcacgtt ttcagtatcg gtcgggcgat ttatgatcag agcgttgaaa
   103261 gtgactcaag ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac
   103321 gcgttcccgt ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca
   103381 ccacctgtag cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt
   103441 gccctggttc agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgctat
   103501 gtgtctggcg ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac
   103561 ctcggctgca gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg
   103621 catcgctggc agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa
   103681 tgagccgtgg ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctgggcctg
   103741 tgcgcacttc acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat
   103801 catcgccttt ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc
   103861 gcgcgtttgc gaggtgattg tcggtatttt gtgcggcggc atgatgatga tgatcctgcc
   103921 gagcagttcc gatgctactg cccttttaac cgcattgaaa aacatgcacg cccgattact
   103981 ggaacatgcc agtttactct ggcagcctga aacaaccgat gccattcgtg cagcacatga
   104041 aggggtgatt gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca
   104101 ctatcgtttt cgccagcaaa acgcgcgcct taatgcgctg ctccaccagc aattacgtat
   104161 gaccagtgtc atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc
   104221 cacacgagaa attctcgaac agttgctgac ggcgctcgcc agttcgcaaa cagatgttta
   104281 caccgtcgca cgtattatcg ccccgctacg cccgaccaac gtcgccgact atcggcacgt
   104341 cgccttctgg cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt
   104401 acatcgtctg caaagcggtg tagatgatca taccagactc ccacggacat ccggcctggc
   104461 tcgtcatacc gataacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat
   104521 gatgattggc gcatggagta ttgcttcgca atgggatgcc ggtgccaatg cattaacgct
   104581 ggcagcaatt agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact
   104641 gctgatgcgc acgctggtgt tactttctct attcagcttt gtggtcaaat ttggcctgat
   104701 ggtccagatt agtgatctgt ggcaattttt gctgtttctc tttccactgc tggcgacgat
   104761 gcagcttctt aaattgcaga tgccaaaatt tgccgcactg tgggggcaac tgattgtttt
   104821 tatgggttcg tttatcgctg tcactaatcc cccggtgtat gattttgctg attttcttaa
   104881 cgataatctg gcgaaaatcg ttggcgtcgc gttggcgtgg ttagcgttcg ccattctgcg
   104941 tccaggatcg gatgctcgta aaagccgtcg ccatattcgc gcgctgcgtc gggattttgt
   105001 cgatcaacta agccgccatc caacactgag tgaaagcgaa tttgaatcgc tcacttatca
   105061 tcacgtcagt cagttgagta acagccagga tgcgctggct cgccgttggt tattacgctg
   105121 gggtgtagtg ctgctgaact gttctcatgt tgtctggcaa ttacgcgact gggaatcgcg
   105181 ttccgatccg ttatcgcgag tacgggataa ctgtatttca ctgttgcggg gagtgatgag
   105241 tgagcgtggc gttcagcaaa aatcactggc ggccacactt gaagaattac agcggatttg
   105301 cgacagcctt gcccgtcatc atcaacctgc cgcccgtgag ctggcggcaa ttgtctggcg
   105361 gctgtactgc tcgctttcgc aacttgagca agcaccaccg caaggtacgc tggcctctta
   105421 attacttaat tacaccacag gcatagcgtt caccgccacc gcccagcggt ttaggttgat
   105481 cggacatatt atcgccgcca acgtggacca tcagcgcttt gtctttgatt tcatccagtg
   105541 atttcagacg aggcgcgatg acggcatcgg tagctttgcc gtcattattg actaccagtg
   105601 caggcagatc gcctaaatgc ccggcacctt ctggcccttc atgtttgccg gtattttgtg
   105661 gatcaagatg cccgcctgcg gattccgcgg cgctggcttt gccatctttg gtggctggct
   105721 ggcagcttcc tttggcatga atatggaagc catgttcacc ggggggtaat gctttcagat
   105781 cgggcgaaaa ctccagacct ttatcggttt cagtaatggt gacgctacca attgactgcc
   105841 caaccccttg cgacgtgacg aggttcatct cgactttttc actggcagct tgtgcgccgg
   105901 ttgcaacaac cagcgccaga atagccagac taaaacgttt cataggacct ccgttcattg
   105961 tgcattcctg aataagtgta aaccagtgac acatttttga acggcggcta ttcctaaaag
   106021 tgcttacggt acgtcgtacc ccagtgccgc tttacggata cgaaaccatt gttgacgggt
   106081 cattttcagt gtttctgctt cgacagctgc ccgtacgcgc tcaattttac ctgagccgat
   106141 aattggcagc ggctgcgacg gtaaacgtaa tacccaggcg taaaccacct gttcaatcga
   106201 gcccgcgttt aactcctccg ccaccacagc cagttcatca cgtagcggct ggaaataatc
   106261 atcattaaac agacgaccac caccaaggca ggaccacgcc atcggacgaa cacgcagttg
   106321 ttgcagttgg tcgagcgtgc catccagcag taacggctga tgtaccgggg atatttccac
   106381 ctgattagtg gcaagggtaa acggcaggcg tgattgcaac agggcaaatt gcgcgggcgt
   106441 gaagttggat acgccaaaat gacgcacttt gccgctctga tgcagatgtt tgaacgcgtc
   106501 cgccacttca tcggcatcca ttaacgggtc cgggcggtgg attaacagca gatccagatg
   106561 atcggtcgcg agattaatca gcgactgttc ggcgctttta ataatatggt cgcggtcagt
   106621 gatgtaatgg ccaatgacgt tttcttcacg cgcagtcgta gcaatgccac atttgctgac
   106681 gatttccatc cgttcacgca gatgtggggc cagtttcagc gcctcgccaa acgccgcttc
   106741 gcactgatag ccaccataaa tatcagcatg gtccacggtg gtcacgccga gatccagatg
   106801 ctcttcaata aaactgacca gctggcgggc ggacatattc cagtccatca atcgccagta
   106861 gcccatcaca aaacgggaaa actccgggcc ttgcggcgca atagtaatac gctgaaccat
   106921 attcgcttcc tcttatcaga tatgagagga gtatacgcaa gattaggctc aaaagagtga
   106981 tggttgctcc ggttcgtctg atgacgctgg cttatttgca cgtaatttgc gcattaatcg
   107041 ctgccgacaa aggcgcagca cctcttgttt ttcgccatcg ctcattttat tccagttaaa
   107101 acgctcatcc cggcttcgaa aacagccgcg acaaaacccg cgttcgtcag actggcaaat
   107161 tccccggcac gggctctgga cgggaaagaa ctctaattgc tccgccactt cgccctcctc
   107221 agataagatt attaccatta ttgaagctgt taatgtccaa agtagcaact ttgcttgcgc
   107281 tagaccgact ggtctactac actccaacac atgaacaaac acaccgaaca tgatactcgt
   107341 gaacatctcc tggcgacggg cgagcaactt tgcctgcaac gtggattcac cgggatgggg
   107401 ctaagcgaat tactaaaaac cgctgaagtg ccgaaagggt ccttctatca ctactttcgc
   107461 tctaaagaag cgtttggcgt tgccatgctt gagcgccatt acgccgcata tcaccagcga
   107521 ctgacggagt tgctgcaatc cggagaaggt aactaccgcg accgcatact ggcttattac
   107581 cagcaaacac tgaaccagtt ttgccaacat ggaaccatca gtggttgcct gacagtaaaa
   107641 ctctctgccg aagtgtgcga tctgtcagaa gatatgcgca gcgcaatgga taaaggtgct
   107701 cgcggcgtga tcgccctgct ctctcaggcg ctggaaaatg gtcgtaatag ccattgttta
   107761 accttttgcg gcgaaccgct acaacaggcg caagtgcttt acgcgctgtg gctgggtgcg
   107821 aatctgcagg ccaaaatttc gcgcagtttc gagccacttg aaaacgcgct ggcacatgta
   107881 aaaaccatta ttgcgacgtc tgccgtttag caggcatttt tttatcacca gacgaccggg
   107941 agcctttatg tcatctgaaa aactgtattc cccactgcaa gtgggcgcga tcacggcggc
   108001 aaaccgtatt tttatggcac cgctgacgcg tctgcgcagt attgaaccgg gtgacatccc
   108061 taccccgttg atggcggaat actatcgcca acgtgccagt gctggtttga ttattagcga
   108121 agccacgcaa atttctgccc aggcaaaagg gtatgctggt gcgcctggca tccatagcca
   108181 ggagcaaatt gccgcgtgga aaaaaatcac cgctggcgtt catgctgaaa atggtcatat
   108241 tgccgtgcaa ctgtggcata ccggacgtat ttctcacgcc agcctgcaac ctgacggtca
   108301 ggcaccggta gcaccttcag cacttagcgc gggaacacgc acttctctgc gcgatgaaaa
   108361 tggtcaggcg atccgtgttg aaacatccat gccgcgtgcg cttgaactgg aagagattcc
   108421 tggtatcgtc aacgatttcc gccaggccat agccaacgcc cgcgaagccg gttttgatct
   108481 ggtcgagctc cactctgctc acggttattt gctgcatcag tttctttcgc cttcttcgaa
   108541 ccatcgtacc gatcagtacg gcggtagcgt ggaaaatcgc gcacgtctgg tgctggaagt
   108601 ggtcgatgca ggcattgaag aatggggtgc cgatcgcatt ggcattcgcg tttcgccaat
   108661 cggtactttc cagaacacgg ataatggccc aaatgaagaa gccgatgcgc tgtacctgat
   108721 tgaacaactg ggtaaacgcg gcattgctta tctgcatatg tcagaaccgg attgggcggg
   108781 aggtgaaccg tatactgatg cgttccgcga aaaagtacgc acccgtttcc acggtccgat
   108841 tatcggcgca ggtgcataca cagtagaaaa agctgaaacg ctgatcggca aagggttaat
   108901 tgatgcggtg gcatttggtc gtgactggat tgcgaaccct gatctggtta cccgcttgca
   108961 gcgcaaagcc ggacttaacc cacagcgtgc tgaaagcttc tatggcggtg gtgcggaagg
   109021 ctacactgat tacccgacgc tgtaatccaa cattgcgagc ggcgcaaagc cgccgctata
   109081 ctaaaacaac attttgaatc tgttagccat tttgaggata taaagatgcg tcttcttcat
   109141 accatgctac gcgttggcga tttgcaacgc tccatcgatt tttataccaa agttctgggc
   109201 atgaaactgc tgcgtaccag cgaaaacccg gaatacaaat actcactggc gtttgttggc
   109261 tacggcccgg aaaccgaaga agcggtgatt gaactgacct acaactgggg cgtggataaa
   109321 tacgaactcg gcactgctta tggtcacatc gcgcttagcg tagataatgc cgctgaagcg
   109381 tgcgaaaaaa tccgtcaaaa cgggggtaac gtgacccgtg aagcgggtcc ggtaaaaggc
   109441 ggtactacgg ttatcgcgtt tgtggaagat ccggacggtt acaaaattga gttaatcgaa
   109501 gagaaagacg ccggtcgcgg tctgggcaac taatctcctg ccgggcgcga actcatcgcg
   109561 cccgcatctt tactgcatcg acaagtaata tttgtcataa tgcgcgctgc aatttatccg
   109621 tattaagaga atcagatgtc cgataacgct caacttaccg gtctgtgcga ccgttttcgt
   109681 ggtttttatc ctgttgtgat cgatgttgaa acagccggat ttaacgccaa aaccgatgcg
   109741 ctgcttgaga ttgccgccat caccctgaaa atggatgaac aaggctggct gatgccggac
   109801 accacattac atttccacgt cgaaccattt gtcggcgcaa atttgcaacc agaagccctt
   109861 gccttcaacg gcattgaccc gaatgatccc gatcgcggcg cggtcagcga atacgaggcg
   109921 ctgcacgaaa tttttaaagt tgtacgtaaa ggtattaaag cgagcggctg taaccgcgcc
   109981 attatggtgg cgcacaacgc caatttcgat cacagcttta tgatggccgc cgctgaacgc
   110041 gcctcactga aacgtaaccc gttccaccct ttcgccactt ttgacactgc tgcactggcc
   110101 gggctggcac tcggacaaac cgtattgtca aaggcttgcc agaccgctgg catggacttc
   110161 gacagcaccc aagctcactc cgcgctgtac gacaccgaac gcactgcggt gctgttttgt
   110221 gaaatcgtca accgctggaa acgtctggga ggctggccgc tacctgccgc cgaagaggtg
   110281 taatcgagtc gatgcctgat gacatgcaat gattcaggca tctatagtga ggctattcca
   110341 cgcatcatgc atgatattca cggggaatag cgttaatggc agataatcca aacctttcat
   110401 cgcccctgcc ggacgtgttt tcaccggcga cccgcgactg gtttcttagc gcctttaaac
   110461 agccgaccgc tgtccagtcg caaacctggc atgtggcggc gcgaggcgaa catgcgctgg
   110521 tgattgcacc gaccggctcc gggaaaacgc tggcagcatt tctctacgcc ctcgatcggc
   110581 tcttccgcga aggcggcgaa gatacccgcg aggcgcataa gcgtaaaacc tcacgcattc
   110641 tctatatttc accgataaaa gccctgggca ccgacgttca gcgcaacttg cagatcccgt
   110701 tgaagggtat tgccgatgaa cggcggcggc gcggcgaaac ggaagtcaat cttcgcgtgg
   110761 ggatccgtac tggcgatacg cctgcacagg aacgcagcaa actcacccgt aatccaccgg
   110821 atattctgat caccacaccc gaatcactct atctgatgct gacctcccgc gcgcgcgaaa
   110881 cgctacgcgg cgtcgaaacg gtaattattg atgaagtcca cgcggtagcg ggcagtaaac
   110941 gtggtgcgca tctggcgtta agtctggagc ggctcgatgc gctgctccac acctcagcac
   111001 agcgaattgg cctttctgcc actgtgcgct cagccagcga tgtggcagca tttcttggcg
   111061 gcgatcgccc ggttacggta gtcaacccgc ccgcaatgcg ccatccgcag atacggattg
   111121 tcgtaccggt cgccaatatg gatgatgtct catcggtcgc cagcggcact ggcgaagaca
   111181 gccatgccgg ccgggaaggc tccatctggc catatattga aacgggtatc cttgatgaag
   111241 tgttgcgcca tcgctcgacc attgtcttta ctaattcgcg ggggctggcg gaaaaactga
   111301 cggcacgatt aaatgagctt tacgccgcac gcttacagcg ttccccttct atcgccgttg
   111361 atgtggccca tttcgagtcg acctccggcg caacctctaa ccgtgtgcaa agtagcgaca
   111421 tttttattgc ccgctcacac cacggctccg tctctaaaga acaacgagca atcaccgaac
   111481 aggcgctgaa atcgggtgaa ttacgctgcg tggtcgcaac ctccagtctc gaactgggga
   111541 ttgatatggg cgctgtggat ctggtgattc aagtggcaac gccgctttct gttgccagtg
   111601 ggttacaacg cattggtcgc gccggacatc aggttggcgg tgtatctaaa gggctgtttt
   111661 tcccccgaac ccggcgtgac ttagtcgatt ccgcagtcat tgtagagtgt atgttcgcag
   111721 gcaggctgga aaacctgaca ccaccgcata atccactcga cgtccttgcg cagcacaccg
   111781 ttgccgccgc ggcgatggat gcattacagg tagacgaatg gtactcccgc gtacgccgtg
   111841 ccacaccgtg gaaagatctg ccaagacgtg tttttgacgc cacgctggat atgctttccg
   111901 ggcgctatcc ctctggcgat ttttctgctt ttcgaccaaa actggtctgg aaccgggaga
   111961 ccgggatatt gtccgcccga cctggcgctc agctgttggc ggttaccagc ggtggcacca
   112021 ttccggatcg tggcatgtat agcgtgttat tacccgaagg tgaagaacag gccggttcgc
   112081 ggcgggtggg tgaactggat gaggagatgg tatatgagtc gcgggtgaac gatattatca
   112141 ctctcggcgc tacctcatgg cggatccagc aaatcacccg cgatcaggtg attgtgactc
   112201 ctgctccggg tcgttctgcc cggcttccct tttggcgtgg tgaaggtaac ggacgtccgg
   112261 ctgaattagg cgagatgatc ggcgattttc ttcatttact ggcggatggc gcgttctttt
   112321 ccgggactat tcccccgtgg ctggcagaag aaaatacgat cgccaatatt caggggttga
   112381 ttgacgagca gcgcaacgcg acgggtattg ttccggggag tcgtcatctg gtcctcgaac
   112441 ggtgccgtga tgaaattggc gactggcgta ttattttgca ctctccctat ggaagacggg
   112501 tgcatgaacc ctgggcgctg gcgattgccg ggcgaataca tgcgctatgg ggcgctgacg
   112561 cgtctgtggt cgccagtgat gacggcattg ttgcgcgcat tcctgacact gatggtaaat
   112621 tgcccgacgc cgcgattttt ttgtttgaac cagaaaagtt gctgcaaatt gtccgcgagg
   112681 cggtaggcag ctcagcactt ttcgccgccc gttttcgcga atgcgccgcg cgggcgttat
   112741 taatgccggg ccgcacgccg ggccatcgca ccccgctctg gcaacaacgg ctgcgcacca
   112801 gtcagttgct ggaaatcgct cagggatatc cggattttcc ggtcattctc gaaaccctac
   112861 gcgaatgtct gcaagatgtt tatgatcttc ccgcactgga acgtttgatg cgtcgcctga
   112921 acggtggcga aattcaaata tccgatgtaa cgactactac gccctcgcct ttcgccacca
   112981 gtttattgtt cggctatgtc gcggaattta tgtaccagag cgacgccccg ctggcagagc
   113041 gccgggcatc cgtactgtca ctggacagcg agttactgcg caatctactc gggcaggtcg
   113101 atccggggga attactcgac ccgcaggtca ttcgccaggt ggaagaagag ttgcaacgac
   113161 tggctcctgg cagaagagcg aaaggtgaag aaggattgtt cgacctgctg cgcgaactgg
   113221 ggccaatgac cgttgaagac ctggcgcaac ggcatacagg cagcagtgaa gagattgcgt
   113281 cgtatctgga aaatcttctt acagtaaaac gcatcttccc ggcgatgatt agcggacagg
   113341 agcgtcttgc ctgtatggat gatgccgcca ggctgcgcga tgccctcggc gtacaactac
   113401 cagagtctct gccagagatt tatttgcata gagtcagtta cccgcttcgc gacctctttc
   113461 tgcgctatct ccgggctcat gctctggtca cggctgaaca actggctcat gagtttagtc
   113521 tcggtattgc cattgtcgaa gagcagcttc agcaactgcg cgaacagggt ctggtgatga
   113581 atctgcaaca agacatctgg gtgagcgatg aagtatttcg tcgtctgcgt ttgcgctcgc
   113641 tgcaagccgc cagagaagcg acgcgtcccg ttgcggccac gacctatgcg cgattgctgc
   113701 tggaacgtca gggcgtatta cccgccaccg atggtagccc ggcgctcttt gcctcaacat
   113761 cgccaggcgt ttatgagggc gtggatggcg tgatgcgggt gatcgaacag cttgccggag
   113821 tcggtttacc cgcctcactc tgggaaagcc agatcctgcc tgcccgcgta cgcgactatt
   113881 cgccagaaat gctcgatgaa ttactggcaa ccggtgcggt tatctggtcg gggcaaaaaa
   113941 aactgggtga agatgacggc ctggtggcac tgcatctaca ggaatatgct gcagaatcgt
   114001 tcactcccgc tgaagcggat caggcgaatc gttcggcgct gcaacaagcg atagtcgctg
   114061 ttctggctga cggaggagcc tggtttgcac aacaaatcag ccaacggata cgcgacaaaa
   114121 tcggcgaatc ggttgatccc tcggcactgc aagaggcgtt atgggcgctg gtctggcaag
   114181 gcgtcatcac cagcgatatt tgggcaccgt tacgcgccct cacccgcagc agttccaacg
   114241 cacgcacctc aactcgccgc agtcaccggg ctcgtcgtgg gcgtcctgtc tatgcacaac
   114301 ccgtctcgcc gcgggtatct tacaacacac caaatctggc tggacgctgg tcgttattgc
   114361 aggtggagcc actaaacgat accgaaagga tgctggcgct ggcggaaaat atgctcgacc
   114421 gctacggcat catcagtcgt caggcggtga tagccgaaaa tatccctggc gggtttccat
   114481 cgatgcaaac gctttgtcga agtatggaag attccgggcg aattatgcga ggtcgttttg
   114541 tagaaggtct gggtggcgcg caattcgctg aacgtctgac tattgaccgc ttgcgcgacc
   114601 tggcgacaca agccgcgcaa acgcgccact atacaccagt ggcgctctcc gccaacgatc
   114661 cggctaatgt gtggggaaat cttctgccct ggcctgcaca tccggcaacg ctggtaccaa
   114721 cgcgtcgggc gggtgcgctg gtggtcgttt ctggcggcaa attgttactc tatctggcgc
   114781 aaggtggcaa aaaaatgctg gtctggcagg aaaaagagga attactcgcc ccagaggttt
   114841 tccacgcgct gactaccgca ctgcgtcgcg aaccacggct gcgctttacg ctaacagaag
   114901 tgaatgatct accggtccgg caaacgccga tgtttacgct gctgcgtgag gcgggatttt
   114961 caagttcgcc acaagggctg gattggggat agagaaagga ctgacggatg cccgttcgca
   115021 tccgtcagta ttgcaggacg gattattccg cgtccggctc ttcagacttg tatttagcgg
   115081 cagtttcttt gatcagctgc tgcagttcgc cacgctgata catttcgatc acgatatcac
   115141 aaccgccgac cagctcgccg tcaacccaca gttgcgggaa ggtcggccag ttagcatatt
   115201 tcggcagttc cgcacgaatg tccggattct gcagaatatc aacataggca aaacgttcgc
   115261 cacatgcggc aagcgcctgg actgcctggg cagagaaacc gcagctcggc agtttcggtg
   115321 aacctttcat gtacagcagg atcgggtttt cagcaatctg gcgttggatt ttttcgatag
   115381 tggtgctcat tgtcttgctt cctcaaactt ctttacggca gtaatctgac attgtagcgg
   115441 gtcagtgcag catcggaaaa taacattttc atcacgcttt agctatttta tccctttgct
   115501 ctatttgttg catttcaaat attcgtttct tctctgatgc gcggcaaact ggcgtattta
   115561 taaccatttt tagctttcac tgctgcattt ttttgcgctc gccaacgaaa cgtatttttt
   115621 aacaataaaa gctattaact ttctcttctt ctatgcatta gaatcatcaa gttttgtaaa
   115681 tcagacgcag gcatgataga cctgccttaa cagagggacg ctcagtggcg cggataaacc
   115741 gtatttcgat cacgctctgt gctttacttt ttaccaccct gcctttaacg cctatggccc
   115801 atgcttcaaa gcaagccagg gagagttctg ctaccactca tatcaccaaa aaagcagata
   115861 aaaagaaaag cacggcaacc accaaaaaga cccagaaagc cagccagaaa acagcgaaaa
   115921 aagccgccag taaaagtacg accaaaagca aaaccgcttc ttccgttaaa aaatcttcca
   115981 ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc cgtcaataaa acggcctcag
   116041 ccagctttac cgaaaagtgt accaaacgta agggctataa atcgcattgt gtgaaagtca
   116101 aaaatgccgc gtcaggaact cttgccgacg cgcacaaagc gaaggtgcaa aaagctacga
   116161 aagtggcaat gaataaactg atgcagcaaa ttggtaagcc atatcgttgg ggtggcagct
   116221 caccgcgtac cggttttgat tgcagcggcc tggtttatta cgcttataaa gatttggtaa
   116281 aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct gcgcgatgca gcgccaatcg
   116341 aacgtagtga actgaaaaac ggcgacctgg tctttttccg tactcaggga cgcggtacag
   116401 ccgatcatgt cggtgtgtat gtcggcaacg gcaaatttat tcagtcaccg cgtacgggtc
   116461 aggaaattca aatcacttct ctcagtgaag actactggca gcgccactat gttggcgctc
   116521 gtcgggtaat gaccccaaaa acacttcgct aaaactttac cctgttgtta cggcaacagg
   116581 gtaagttcat cttttgtctc accttttaat ttgctaccct atccatacgc acaataaggc
   116641 tattgtacgt atgcaaatta ataataaagg agagtagcaa tgtcattcga attacctgca
   116701 ctaccatatg ctaaagatgc tctggcaccg cacatttctg cggaaactat cgagtatcac
   116761 tacggcaagc atcatcagac ttatgtcact aacctgaaca acctgattaa aggtaccgcg
   116821 tttgaaggta aatcactgga agagattatt cgcagctctg aaggtggcgt attcaacaac
   116881 gcagctcagg tctggaacca tactttctac tggaactgcc tggcaccgaa cgccggtggc
   116941 gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt ttggcagctt tgccgatttc
   117001 aaagctcagt ttactgatgc agcgatcaaa aactttggtt ctggctggac ctggctggtg
   117061 aaaaacagcg atggcaaact ggctatcgtt tcaacctcta acgcgggtac tccactgacc
   117121 accgatgcga ctccgctgct gaccgttgat gtctgggaac acgcatatta catcgattat
   117181 cgcaatgcac gtcctggcta tctggagcac ttctgggcgc tggtgaactg ggaattcgta
   117241 gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag cattgcctga tgcgcttcgc
   117301 ttaccatgcc aacgcggatg aggcgttaac agcgcattcg gcaagataaa tcgcacgttg
   117361 tcagcaactg taacgcagaa ggttatcctt ctgcgttttt gtttaattag ctgttagcaa
   117421 cgcaaactgt ttcaggttgt tttctggctg acataaacac cagcaataat gccagtcccg
   117481 cgacaatcgc ccccatcacc ggcacaaagc tgtatcccag cccagcggaa attaccgcac
   117541 caccagcggc tgctcccagc gcatttccaa gattaaaggc accaatattg actgatgaag
   117601 acagacctgg cgcttcactg gcgacacgca tcacgcgcat ctgtaacggc ggtacgaccg
   117661 caaaggttgc agcgccccac accaccatgc taatagctgc gccgaactca ttgcgggcca
   117721 ggaacgggat tgccagcata atcaccatca gcagcaacaa aaagcctttc aacgtgccgt
   117781 taactgaacg atctgccagt ttgccgccga gatagttacc gatagagaat ccgacaccaa
   117841 tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg ggtaatactt tgcagtaccg
   117901 gagagatata ggtgtagaga gtaaacattg caccagctcc cagtaccgtc gtcagcaatg
   117961 cagacagcac ctgcggacgc attaataccg ccagctcttt tttcacttca ggtcgtgccc
   118021 ctgcaccacc tttaggtaat gagaagaaca gacttaccat tgaaatcact cccagccccg
   118081 ccgttgccag aaatgacatc cgccagccga tggtttcacc caaccaggtc gccgccggca
   118141 cgccaccgat atttgccagg gttaacccca taaacatagt ggcaactgcg ctggcctgtt
   118201 tatgttttgg caccacgctt gcagccacga ctgaacccaa accaaaaaat gctccatgat
   118261 tcaggctggt caaaatgcgt gaaagcatca gggtcatata atccggcgcg atggcggaaa
   118321 gtacgttgcc gagcgtgaaa attgccatca ggaaaatcaa cgcactgcgg cgggcacgat
   118381 gagaaagtag aagcgtcatc agcggcgcgc caaccattac gccaactgca taggcactga
   118441 ttaacattcc ggcagcggga atcgagacat ccacaccgcg cgcaatgacg ggcaacaagc
   118501 ccattggcga gaactccgtt gtcccgatac caaacgcgcc aatcgccagc gccagcaacg
   118561 gatagttaat tttcatgcct tatctccacc tcttcgcgtc attacgcgat attcattaaa
   118621 gtggcgaaag catgacagca atcacaaaaa aatgaaaata acaaaaagag aaaacacttt
   118681 tgccattttg ctaacaaaca ggaaggagat gcgagggaga acgcgctccc tcgagaggaa
   118741 atcagtgcag cgcggcagtc aaacccacgg ctacgatcaa accgaggacg ataatcgttg
   118801 ttaccagtga aaatttaagg tcggtgctca tcaagttttc tcctttttta ttaccacaca
   118861 aaaagtgata ttacgcattt ttacacactg tgatgaaaaa atctcccgtc atttataatg
   118921 ataagtgttt ttaccacttc cccttttcgt caagatcggc caaaattcca cgcttacact
   118981 atttgcgtac tggccattga ccccttcctg acgctccgtg tcgtttttcc ggcgtaccgc
   119041 aacacttttg ttgtgcgtaa ggtgtgtaaa ggcaaacgtt taccttgcga ttttgcagga
   119101 gctgaagtta gggtctggag tgaaatggaa tggcaacaat aaaagatgta gcgaaacgag
   119161 caaacgtttc cactacaact gtgtcacacg tgatcaacaa aacacgtttc gtcgctgaag
   119221 aaacgcgcaa cgccgtgtgg gcagcgatta aagaattaca ctactcccct agcgcggtgg
   119281 cgcgtagcct gaaggttaac cacaccaagt ctatcggttt gctggcgacc agcagcgaag
   119341 cggcctattt tgccgagatc attgaagcag ttgaaaaaaa ttgcttccag aaaggttaca
   119401 ccctgattct gggcaatgcg tggaataatc ttgagaaaca gcgggcttat ctgtcgatga
   119461 tggcgcaaaa acgcgtcgat ggtctgctgg taatgtgttc tgagtaccca gagccgttgc
   119521 tggcgatgct ggaagagtat cgccatatcc caatggtggt gatggactgg ggtgaagcaa
   119581 aagctgactt caccgatgcg gtcattgata acgcgttcga aggcggctac atggccgggc
   119641 gttatctgat agaacgcggc catcgcgaaa tcggcgttat ccccggcccg ctggaacgta
   119701 acaccggcgc aggccgcctt gccggtttta tgaaggcgat ggaagaagcg atgatcaagg
   119761 tgccggaaag ctggattgtg cagggtgatt ttgaacctga atccggttat cgcgccatgc
   119821 agcaaatcct gtcgcagccg catcgcccta ctgccgtctt ctgtggtggc gatatcatgg
   119881 caatgggcgc actttgtgct gctgatgaaa tggggctgcg cgtcccgcag gatgtttcgc
   119941 tgatcggtta tgataacgtg cgcaacgccc gctattttac gccggcgctg accacgatcc
   120001 atcagccaaa agactcgctg ggtgaaacag cgttcaacat gctgttggat cgtatcgtca
   120061 acaaacgtga agaaccgcag tccattgaag tgcatccgcg cttgattgaa cgccgctccg
   120121 tggctgacgg cccgttccgc gactatcgtc gttaatcacc cgttgcggga gtctcttccg
   120181 gctcccgcag ccactcctta ttcagcgtct cactatcgcc gagatactca agcaaccagg
   120241 ttaacgcagg cgacatatca ttttgctgcc atgtcagaca acatgccgaa tccggaaagg
   120301 ggttttccag ttctaatgct acccacttcc cctcattaag ccacggtttg gcgaaatgtg
   120361 ttggcaccat ccctatgcat aatcctgccg agatacaggt tgccgatgat tcccagtcag
   120421 gcacgacaag tcttttttgg ttatccagca accaggtaat acgcttaggt agcgttcgcg
   120481 aggtgtcttc gcgcaccaac gacggccagt tgcgcaacgt atcatcgctg aacgggccat
   120541 ccatcaacgc cagcgggtga tggctggcaa caacgcaact ccagcttagc atccccatat
   120601 cccggaaggc ataacgaccg cctaccggaa tcgcgcgtgt tgcgccaatc gccagttcca
   120661 cgcgcccgtc ggaaagcgca tcccagacac cgttgaacac ttcctgaaag acaagaagtt
   120721 cgacatcatc aaaatggcga taaaaatcaa cgatcatctg ccgtgtacgt tctggcctga
   120781 caatattatc cactgcaata gctaactgac cacgccagcc gttcgctatc tgctgacatt
   120841 gctggcgggt gatctgcatt tttttgacaa cagagcgacc ttctttgaga aaccacgccc
   120901 cggcagcggt cagttccaca tcacggtgac gtcgttcaaa cagcggcacc gccagccact
   120961 cttccagctg acgtacggta tagctgaccg cagaaggaac gcgatgcaac tcctgggctg
   121021 cggcactaaa actaccatta cgcgctaccg catcaacaac ttcgagtgaa tattctgacc
   121081 acatagtctg cctgcaaaat ttttgaaacc agtcatcaaa tattaccgtt tcacaacact
   121141 aatttcactc cctacacttt gcggcggtgt ttaattgaga gatttagaga atatacatgc
   121201 aacctgggaa aagattttta gtctggctgg cgggtttgag cgtactcggt tttctggcaa
   121261 ccgatatgta cctgcctgct ttcgccgcca tacaggccga tctgcaaacg cctgcgtctg
   121321 ctgttagtgc cagccttagt ctgttccttg ccggttttgc cgcagcccag cttctgtggg
   121381 ggccgctttc cgaccgttat ggtcgtaaac cggtattatt aatcggcctg acaatttttg
   121441 cgttaggtag tctggggatg ctgtgggtag aaaacgccgc tatgctgctg gtattgcgtt
   121501 ttgtacaagc tgtgggtgtc tgcgccgcgg cggttatctg gcaagcgtta gtgacggatt
   121561 attatccttc acagaaagtt aaccgaattt ttgcgaccat catgccgctg gtgggtctat
   121621 ctccggccct ggctcctctg ttaggaagct ggctgctggt ccatttttcc tggcaggcga
   121681 ttttcgccac cctttttgcc attaccgtgg tgctgattct gcctattttc tggctcaaac
   121741 ccacgacgaa ggcccgtaac aatagtcagg atggtctgac ctttaccgac ctgctacgtt
   121801 ctaaaaccta tcgcggcaac gtgctgatat acgcggcctg ttcagccagt ttttttgcat
   121861 ggctgaccgg ttcaccgttc atccttagtg aaatgggcta cagcccggca gttattggtt
   121921 taagttatgt cccgcaaact atcgcgtttc tgattggtgg ttatggctgt cgcgccgcgc
   121981 tgcagaaatg gcaaggcaag cagttattac cgtggttgct ggtgctgttt gctgtcagcg
   122041 tcattgcgac ctgggctgcg ggcttcatta gccatgtgtc gctggtcgaa atcctgatcc
   122101 cattctgtgt gatggcgatt gccaatggcg cgatctaccc tattgttgtc gcccaggcgc
   122161 tgcgtccctt cccacacgca actggtcgcg ccgcagcgtt gcagaacact ctacaactgg
   122221 gtctgtgctt cctcgcaagt ctggtagttt cctggctgat cagtatcagc acgccattgc
   122281 tcaccaccac cagcgtgatg ttatcaacag tagtgctggt cgcgctgggt tacatgatgc
   122341 aacgttgtga agaagttggc tgccagaatc atggcaatgc cgaagtcgct catagcgaat
   122401 cacactgatc tatatcgata tacttatact taggctgcta acaaaatttt gttgtatgat
   122461 tgaaattagc ggcctatact aatttcgagt tgttaaagct acgataaata ttatgttttt
   122521 acggggacag gatcgttccc tactcactat ggatggtcat ttcggcaagg gttcctcctt
   122581 tccctctgtt ctacgtcgga ttatagactc gcggtttttt ctgcgagatt tctcacaaag
   122641 cccaaaaagc gtctacgctg ttttaaggtt ctgatcaccg accagtgatg gagaaactat
   122701 gagttcatcg tgtatagaag aagtcagtgt accggatgac aactggtacc gtatcgccaa
   122761 cgaattactt agccgtgccg gtatagccat taacggttct gccccggcgg atattcgtgt
   122821 gaaaaacccc gattttttta aacgcgttct gcaagaaggc tctttggggt taggcgaaag
   122881 ttatatggat ggctggtggg aatgtgaccg actggatatg ttttttagca aagtcttacg
   122941 cgcaggtctc gagaaccaac tcccccatca tttcaaagac acgctgcgta ttgccggcgc
   123001 tcgtctcttc aatctgcaga gtaaaaaacg tgcctggata gtcggcaaag agcattatga
   123061 tttgggtaat gacttgttca gccgcatgct tgatcccttc atgcaatatt cctgcgctta
   123121 ctggaaagat gccgataatc tggaatctgc ccagcaggcg aagctcaaaa tgatttgtga
   123181 aaaattgcag ttaaaaccag ggatgcgcgt actggatatt ggctgcggct ggggcggact
   123241 ggcgcactac atggcgtcta attatgacgt aagcgtggtt ggcgtcacca tttctgccga
   123301 acagcaaaaa atggctcagg aacgctgtga aggcctggat gtcaccattt tgctgcaaga
   123361 ttatcgtgac ctgaacgacc agtttgatcg tattgtttct gtggggatgt tcgagcacgt
   123421 cggaccgaaa aattacgata cctattttgc ggtggtggat cgtaatttga aaccggaagg
   123481 tatattcctg ctccatacta tcggttcgaa aaaaaccgat ctgaatgttg atccctggat
   123541 taacaaatat atttttccga acggttgcct gccctctgta cgccagattg ctcaatccag
   123601 cgaaccccac tttgtgatgg aagactggca taacttcggt gctgattacg atactacgtt
   123661 gatggcgtgg tatgaacgat tcctcgctgc atggccagaa attgcggata actacagtga
   123721 acgctttaaa cgaatgttta cctattatct gaatgcctgc gcaggtgctt tccgcgcccg
   123781 tgatattcag ctctggcagg tggtgttctc acgcggtgtt gaaaacggcc ttcgagtcgc
   123841 tcgctaaagg ctattctatc gcccccgctc cgggggcgat ttcagatcag gcttctgtac
   123901 ctggttgatt catggcattt tctcgtgccg ccagcacacg ttctaccgta tctaccactg
   123961 cctgagtttg tggatcgatt tcaatgttga cgcgtgcgcc aagttttttc ttcccaagag
   124021 tcgtgcgttc cagtgtttcc ggaattaaat ggacgcaaaa acgcgttggc gtgacttcgc
   124081 cgacggtcag gctaataccg tcgatgccaa taaatccttt gtacaggata tatttcatca
   124141 actgactatc ctggacttta aaccagatct ggcgattatt ttccgaggtt aatattttcg
   124201 ccacttcagc ggtggtcata atatgacctg acattaagtg tccgccaatt tcatcactga
   124261 atttcgccgc acgctcaacg tttacccaat cccccacttt taaatcgcca agattggtaa
   124321 tgcgtaacgt ttctttcatc aggtcaaaac tgacatggtt gccgttaatt tccgtcacgg
   124381 tcaggcagca accgttatgc gccacggacg caccggtttc caggccgtcc agcatgtggt
   124441 cgggtaactc caccacatgc gtacgaaaat ttggtttctc gtcaatcgac accagttttg
   124501 cggtgccctg tacaatcccc gtaaacatac ttacaactcc tgaaatcagt taagacattc
   124561 tgttcagcac aatagcaggt ggcaaacgcc cttaccagtg aaggggtaag aatgactatt
   124621 ttttcactgg agaattaata aatcctcgct acaatagact gaatttcccc tgcttcttct
   124681 ttttgctgcc cattcaggcg gctttttagt ctctcatata actacaaata aaaggtgttc
   124741 acgtgcagaa gtatatcagt gaagcgcgtc tgttattagc attagcaatc ccggtgattc
   124801 tcgcgcaaat cgcccaaact gcgatgggtt ttgtcgatac cgtgatggcg ggcggctaca
   124861 gtgccaccga catggcggcg gtcgctatcg gtacttctat ctggcttccg gcgatcctct
   124921 ttggtcacgg actgctgctg gcattaacgc cggttatcgc gcaattaaat ggttccggtc
   124981 gacgtgagcg cattgcgcat caggtgcgac aaggtttctg gctggcaggt tttgtttccg
   125041 ttctcattat gctggtgctg tggaatgcag gttacattat ccgctccatg gaaaacatcg
   125101 atccggctct ggcggacaaa gccgtgggtt atctgcgtgc gttgttgtgg ggcgcgccgg
   125161 gatatctgtt cttccaggtt gcccgtaacc agtgtgaagg tctggcaaaa accaagccgg
   125221 gtatggtaat gggctttatc ggcctgctgg tgaacatccc ggtgaactat atctttattt
   125281 atggtcattt cggtatgcct gagctcggtg gcgttggttg tggcgtggct actgcggcgg
   125341 tgtattgggt catgttcctt gccatggttt cttacattaa acgcgcccgc tccatgcgcg
   125401 atattcgtaa cgaaaaaggc accgcgaaac ccgatcctgc ggttatgaaa cgactgattc
   125461 aactcggttt gccgattgcg ctggcactat tctttgaagt gacactgttt gccgtcgtgg
   125521 ctctgttagt gtctccgctc ggtattgttg atgtcgcagg acaccagatt gccctgaact
   125581 ttagttcact aatgttcgtg cttccaatgt cgctggcggc agcggtaact atccgcgtag
   125641 gttatcgtct gggtcagggc tcaacgctgg atgcgcaaac cgctgcgcgg accgggctta
   125701 tggtgggtgt ctgtatggca accctgacgg ccatattcac ggtttcactg cgggagcaaa
   125761 tcgccctgtt gtacaacgac aatcccgagg ttgtaacgct ggctgcgcat ttgatgttgc
   125821 tggcggcggt atatcagatt tctgactcaa tccaggtgat tggcagtggg attttgcgtg
   125881 gttataaaga tacgcgttcc attttctata tcacctttac ggcttactgg gtgctgggct
   125941 tgccaagcgg ctatattctg gcactgaccg atctggtcgt tgaacctatg gggccagcag
   126001 gcttctggat aggctttatt attggcctga cgtcggcagc cattatgatg atgctgcgta
   126061 tgcggttcct gcaacgtatg ccgtcagcca tcattctgca acgagcgtcc cgctaataaa
   126121 gacaaggcgc aaccttcacg ggttgcgcct gtatttttac gcaggctgga gcgttgcgcc
   126181 aatcccgtct tcgtctggct gtagtttcag tgcattacac agagtttgcc gactggcatc
   126241 tgttatctca acgcgtatat ccccggtaac ttcccctttc acattgatga agtcaggtga
   126301 aatttctttc gcactggttt gcaggcaaat cgctccctct ccgcgcaggc gtttcacttt
   126361 cagttgcaca tgcgttgccg caccagtgat cagccacagt tttccgccat tcagattgat
   126421 gttaccaata ctgaaagtga cactgtcacc ttttaccgta ctgccgtcga gaatcaatac
   126481 accgccctgc tgtacagtaa tcgttgattg ttgcagatct gcgccatagc caataatcat
   126541 tgaagcgccg tccagtaatg caacactccc ggtcagttca accggtgcgc gatcttcctc
   126601 tacgacaaaa ttttcgtccg cgctggtggg tgacaacagg atcactgttc caccttgtat
   126661 taccgcattt tccagcgtac cggcatgcac ttcagcccga ccacccacat taacggacag
   126721 gttttgcgtc ttaccggaac tgtagagctg aagaccatct gtccccagac gatagatggc
   126781 tccattctca ataatagtgt cagagtcttc accaccgaga ttattcatgg tgccaccagc
   126841 aagcaacgta tcactggctt tccccgccag cataaaaacg ccgccctgat taatctcaca
   126901 attgctggct tccccacctg gatacaccaa caattcacca ccttcatcta ccacgaccgc
   126961 tgaggttgtc ccattgacca acaggccgcc ttcttgatcg agaataattt tttccgcgcg
   127021 atgtccttcc agtacacgca ggttaccgcc attttccagc aacaaaccgc aggcataacc
   127081 tttatcaacg ctgaattcac cttcgggatg gcggccatta accgtagcga gcgtagagag
   127141 actaattgcg cctccctcgg actgaacaat atctgtggca atggcaccgt cgctgaccga
   127201 tagcgtgcca ttgttaccgt actgtgtgca tttggcgatc ccaccggttg ccactttcaa
   127261 ccagccagcc cccgtgacca cggtattttc ggctcttccg ccagcttcaa caatcaactc
   127321 gccttcagta agttgcgtcg cgccaatgac ctcgccggtc ttaacaattt gcacattccc
   127381 gtcgctcatc aagtttttcg catcagatcc catgatttat tcctttgctg catctgtgtg
   127441 cctttattgc tacctaagtg taaaggctac ggaggattta tccacgacag atttgagatg
   127501 gtggcaaaca actctgttta aactctgata cacgaattat tgggttgtat cagatgtaaa
   127561 tgcgatcctg aataaaaatc gcccttgcaa atcaacaaaa tatagccaat ttgcttaaaa
   127621 gctctgcaaa cggtcgaaat agcgcagaaa attacgtttt gcctcttgcc accttcccac
   127681 tctgccgcta atattcgtcc ccgttgtcac ctacaacgtt gcgttcatag ctcagttggt
   127741 tagagcacca ccttgacatg gtgggggtcg ttggttcgag tccaattgaa cgcaccatcc
   127801 tgcgtccgta gctcagttgg ttagagcacc accttgacat ggtgggggtc ggtggttcga
   127861 gtccactcgg acgcaccaga ttttcttaat ctggtcttct cctttttccc tctgcttctt
   127921 ctctgtatcc aatacgttaa aagatttaca ctgtcttcgt atgcgttatc agaaggagaa
   127981 tcgctatggc aactttgtta caacttcatt tttctttcaa tggcccattt ggcgacgcaa
   128041 tggctgagca gcttaaaccg cttgctgagt cgattaatca ggaacctggt tttctgtgga
   128101 aggtatggac cgaaagtgaa aagaaccacg aagccggtgg gatctacctt ttcactgatg
   128161 aaaaaagcgc ccttgcctat ctggaaaaac acactgcccg actgaaaaat ctcggcgttg
   128221 aggaagttgt cgccaaagtt ttcgatgtca atgagccact gagtcaaatc aatcaggcaa
   128281 aactcgcctg acagatttta atcaagggcg gttagcgccc ttttcatccc tgtctgaaat
   128341 ttctcaaatt ctaaaaattt caaccaaact tatctgataa cactaaattc gaaagaatgc
   128401 gtacaggtaa gtaacaatga aaaaaattgc tattgtgggt gccgggccta cgggtatcta
   128461 caccttattc tcgcttctac agcaacaaac tccactttct atttctatct tcgagcaggc
   128521 tgatgtggcc ggtgtcggga tgccatacag tgatgaggaa aactcaaaaa tgatgctggc
   128581 aaatattgcc agtattgaaa taccgccgat ttattgtacg tatctcgaat ggctacaaaa
   128641 gcaagaagcc agccatctcc agcgttatgg cgttaaaaaa gaaaccttgc acgatcgtca
   128701 gtttttaccg cgaattctgc tgggcgaata tttccgcgat caatttttac gattagtaga
   128761 ccaggcacga aagcaaaaat ttgcagtggc tgtttatgaa tcatgccagg ttaccgatct
   128821 gcaaattaca aatgctggcg tcatgctcgc tacaaatcag gatttaccca gcgagacgtt
   128881 tgatttagcg gtgattgcca cgggtcacgt ctggcctgat gaagaagaag caatccgaac
   128941 gtattttccc agcccgtggt caggcctgat ggaagcaaag gtcgatgcgt gtaacgtggg
   129001 tattatggga acatccttga gcggactgga tgcggcaatg gcagtggcaa ttcagcatgg
   129061 ttcgttcatt gaagatgata aacaacacgt cgtttttcac cgcgataacg caagtgaaaa
   129121 gctaaatatt acgttaatgt cgcgcacggg tattttaccc gaagccgatt tctattgccc
   129181 tattccctac gagcccttac acatcgtcac cgatcaggca ttaaatgctg agattcaaaa
   129241 aggcgaatat ggccttttgg atcgggtatt tagattgata gtagaggaaa tcaagtttgc
   129301 tgatccagac tggagtcaac gcatagcctt agagagcctg aatgtcgatt cctttgctca
   129361 agcctggttt gccgagcgca aacaacgcga cccatttgac tgggcagaaa aaaatctcca
   129421 ggaagtcgaa cgcaataaac gagaaaaaca tactgttccc tggcgttatg tcattctgcg
   129481 tctgcatgaa gccgtacagg aaattgttcc acatctgaat gaacacgacc ataaacggtt
   129541 cagtaaaggc cttgcccggg ttttcatcga taattatgcg gcaatccctt cagagtctat
   129601 tcgtcgccta cttgccttac gtgaagcggg aatcattcat attctcgctc tcggtgaaga
   129661 ctacaaaatg gaaattaacg agtcgcgcac cgtcctgaaa acggaagaca acagctactc
   129721 gtttgacgtt tttattgatg cccgcgggca gcgtccgctt aaagtgaaag atattccttt
   129781 ccctggacta cgcgaacaat tacagaaaac aggggatgaa atccctgatg ttggtgaaga
   129841 ttatacgtta cagcaacccg aagatattcg tgggcgcgta gcgttcggcg cgttgccctg
   129901 gttgatgcac gaccagcctt tcgttcaggg acttacggca tgtgcagaaa ttggtgaggc
   129961 gatggctcgg gcggtcgtaa aacctgcctc ccgtgcacgt aggcgtcttt cgtttgatta
   130021 aaggtaaagc tatcagcact caacaaactg atgctgttca ttaagacgat agcggactcc
   130081 ggctcgaata ttgttttcac cttcaatggc gacggcgaaa cgaactcttt cgccgtcatg
   130141 atatgccagt gcggcgcttc ctcctggccc gagaataatt gagtcgacca cgcccgtgct
   130201 cactaccgtt gaattttcac cgctggcaat gatgtgtaca ttgtcgccgc tgttggcaat
   130261 tctggcattt gcgccaaagc tggcgatttg caccaggtca ccattactag cgacatgaca
   130321 tcgttcaccc aatgtacaaa cgcgtactcg cattccggta ttcgcaatcc gactgctatc
   130381 tccagcgctg cttatccgcg aagaattgcc ggttacagca atccgtgcgc gctcaccgga
   130441 gctgccaatg tgactgttaa agccgacact tccaatccgc acgctataac ccatactggc
   130501 aatttgtgca gcatacccag cggttgcgat tctggcgtta tcctcacaac agccgatgcg
   130561 agcatgatcg tcgctgcggg caaactgact gcaaaatgct ctctctccgg gatctgtggc
   130621 cagtttttgc atagaggaaa cttcctgatg cgcgaagttt tcatccgcca gccatttttt
   130681 ccacgcatac tcaacaagac tctccgccca gtccgtatat ccttcctcaa tcagtgcatg
   130741 atgaatatca gcataactac cgccgcgcgg gaaatggcgc aggaaccagc gatacatcac
   130801 cgctccgata cgccattctc gcaggtctgc tcgtgtaatg atcattcgtt agtgccggtg
   130861 atagccatcg accatgcttt taaaggtttc gtgtggcgta cgccccgtgg tgcaaagata
   130921 aagatgaccg aagataaaaa agaggcttat aaatgccaga gcaaaatgcg cctgcaataa
   130981 ccagtatctt acgccaggaa acacatctcc cacggcttgc ggatagagac acagcagccc
   131041 cgtcaatagt aacaacggca gcaatccata catgacacca acataggcaa cctgctgtaa
   131101 gggattaaat ttagactggg ttgttgccgg gaaaggatgt tcttccccct gcataatgcc
   131161 aaacaaataa aatcgcgttt gttttgccgc ccgctccagc caaccctgac gacgaatgcg
   131221 atagtggtgg ccattacccc caacggcatt gatcagaaca aagccgagcc agcatgccag
   131281 taacaaaaat ccgcaaactt catgcaccgc aaccagactt ttaaccgcag ttgcgcccac
   131341 cagcgcaaag tgatttatca gcccacttgc cagcaacaat acaaagagta acgcattcga
   131401 ccagtgccat aatctgaccg ctttgctgta aagataaatc ttctcaccat gatcggtggc
   131461 tgatttcttc acgccacgag cacgaagcaa ggcgtgcagc cccagcacca accacatccc
   131521 aaccagcaat actccggcaa tgatcaacca caccggccaa aattcgggag tgaactgtgg
   131581 cacatagttc gctaactggc tctgaaactg ttcagcatgt tgcgacgggt tcatacattt
   131641 tcctttttaa tcaaatgttg accgaaccgg cgatacagat gcggtttccc ggcaccaggt
   131701 agctgatatt gatagtattt attatcctgc aaccacgcct gaatttctgg gctatcttcg
   131761 cgaccaaaga tcaatgcgtg ttccgggcag gcgctgacgc aaatgggcgg aaagcctttt
   131821 gccagtcggg actcagcgca gaaatcacat ttatctgcaa ctttagtgac agggttaagg
   131881 tagcgaacct ggtacggaca agcgccaatg caataactgc agccgatgca ctgcgatttt
   131941 tctacccgca cgatcccctg ctcatcacgc cacgacgccc ctgtcgggca tacgtcgatg
   132001 cacggagcat cttcgcagtg ctgacatgac tggcgaaaaa agtgatactg cgtttcgttg
   132061 tcgttatcgg tgactggaat atgtgctatc gataaacggc ttccctgagc ggggacgtgg
   132121 ttagttttac ggcatgcgcg ggcgcaaata ttacaaccat tgcaccgtga ctcgtcatga
   132181 atcatggcat accgaacttc cttttcttgc ctcgtgttcg ctaacagaga agacgcagaa
   132241 ccagtaaaaa agattactgt tcccatcccc agaacaaatt tgcgtcgagt gaaggacatc
   132301 tgattatccc ctaatgcagt ggctgcaact gtccacccta tccatgacat ctccctgaaa
   132361 tttcaggggt tctgaaactg ctctacctcg aaccattcgc ataagccata agccttgcca
   132421 taggtcataa atcgctcgct aaacaagtac aacaccgccg tcgatgactc cacctgccgt
   132481 atatcggcat attcaggtgc aacatccatc gcggcgatcg cggcttcaat ctgcgcttcc
   132541 tggaagtaat atggcgcttg catcagcatg gcgactttgt aaggacgagg atacgtctgg
   132601 cactcaaaac gaacggcgtg tgcaatggcg cgacaaatgt cctgctccac aacttgcaaa
   132661 gacatcgccg cgtagttttc actcatggcc tgcgttgagt aatagtaatc atcctgactt
   132721 cccgtcatac aagcgatatc cgcgtagtct ccgttctgct taatgctttc cagcaaaact
   132781 gacagttcct cttctgcaac cgaatagggt ggcgagagaa acacggcgcg cgccaccagc
   132841 tgcccagcca cagaatgctg gcgaatgaac tctgccagga tttcagccgg ttctggttcc
   132901 gttaccgtat caacggggtg acgcatcccc tgcaaccatt gccgcttcgc ctcgtcaact
   132961 tcgctgactt tcgctaacgg caattcatcc tgatggttca tcttacccat tccttacgca
   133021 ggcagtagat tgtgtgctgc cagatcggcg gcaatatctt ccagccccag acgctgcaat
   133081 gtttcgcggg ttgggcaacc aagctgtggg tcccagccca tttctttgta gaacatcgtg
   133141 agcgaagcat gcatatcgtc acgatccatt ttgtcagtac cttcagtaaa gaccgggatc
   133201 tgcggatcct tgtcgaatac ccaggaacag ataagatcgt gttcattacg catatctttg
   133261 gtttgcatca gtttcaccgt gtaggcacga tgcaatgtaa agatacgctc tgcagctaaa
   133321 tctaattttt cctgagtcat ctcttcgcca gtgatcgctt tgaagaattt ggcttcaagc
   133381 gccagatcgc cccggtaatt acggcttttc agcggcgaaa cggtcattgg ccagacccag
   133441 ttgcacaggg tgacggcgtt atgcaaacag acccgcaaca gcgaccactt agcatattta
   133501 atttttgcgt cattgatcgg cgtgtagttt ttggtttcat cgtaagcatc ttcagatcca
   133561 aaaagttctt tcgccacttc acgttgcagt ttcaatggca agccggaacc aataaagttg
   133621 atatgggtat gcgtcatgca gtcacggttg aacatacagt taacaatgga accgacctgc
   133681 gctgacgctt cattggcatg gtgaaccgga tagccaaacg gcgaccagag tttatttttc
   133741 gcgtagcccc agtactcttc acccaaattc cagcgttctg cgatggcata tgaaccatca
   133801 gccaggtggc tcagctcacc cacacgatgc gccagacggt agtaaaaatc tttaatgaag
   133861 ttaacgtcac ccgcttccag ttgatcccag cgaatttccg catactcttc agctggcaga
   133921 acacgcttga acacaccttt gctgtaacaa taagtaaagt cgcgatgcag ctgcccgtag
   133981 ttacaccaca ggccgtagtc atcgaacaaa ttcagaccaa ccaggttacc aatcacacga
   134041 ccatcgtctt tatcttcaaa atctttcggc ccgttcggga agatggtggt atggacaaag
   134101 tttgctacac aagtgttacc acctgtactg ggcacgccaa actcttttac ccgaggaata
   134161 ttcatttggg tcatacaacg gatcgggcaa gagtggcagc cgctcatttt tactgtgtat
   134221 ttctctgccg ccggacctaa gtcaaaaacg gatttatagg tacgaaagcc gaccgtattc
   134281 tgattgcctg gcggaatttc acccgtttca attggaccgc cttcagccgc gccccaaaac
   134341 agccctttac gtgctgtcca gcgtgacttg ggatctgaat actctgccca cgattgcggc
   134401 gtacttggca cgacatggtt gttattcgca ccgataagtt cagtcatcat gtaatcattg
   134461 agacgcttca tctcctgacg atcggcaatg ttgacccctt tcgtcccttc aaccgcaatc
   134521 gctttcaggt ttttcgaacc cattattgcg ccagttcccg caccaccgct gtggttacgg
   134581 ctattcaaca tgccagagag aggaacaagg ttttccccag cctgaccaat agccgccaca
   134641 caggtttccg gactggtcaa tcgacaaatt tcttccgtcg ttgcgcgcgt cccttttccc
   134701 cataagaaat cggctttttc caggctaact ttgtcatctt taatcttcag ccataccggt
   134761 gatttcgcct tcccttcgat aataatgacg tcgtatccag cgaatttcat ttgcgctgca
   134821 aaaaagcccc ccatatgggc atcgacgact aaatttcctt tggtaaaagt agaaagtgag
   134881 gtgatattta cgcgagaact acagggggca ccagatccag ttaatgggcc ggtagcaaag
   134941 actaatttat tcgcttcatc gaaaggtttc gtgcctggcg gtacttcgtc atacataatt
   135001 ttgtagccga agcccatgcc accgacaaaa cttttaaact tactggaatc ttcgagggta
   135061 atatttcctg tcgtgagatt gactcttaat atattacctg tccaaccgtt agccatgatt
   135121 ttttcctttg caagattcac acagtaatat ctttccactc gataattttt aacgcccctg
   135181 ttgggcaggc gtttgcgcat tcaccgcata acacacattt cgaggatttt ttactttcgg
   135241 tatttacggt ggccatcatc cacgggcatg ccgtagtaca ggcgctacag ccaatacaac
   135301 gtttatgatc gacggtaata cagccttctt tctgctgcca ggtaatcgca ccaatcgggc
   135361 agacgttcat gcattgcggc tctttgcatt gacggcaggt gtccgcggta tagttgagat
   135421 cgccatacag gccgccgcca gagccaaccc cgttgtcgcc aaagaaataa ttgcgatgga
   135481 ttttgatacg ggagaagaat gtccctactg agccatcgtt gaagttggta caagagattt
   135541 cacagcggtg gcaacccgtg catcgcgctc gctgggtcac cagcacccct ttgggtgtat
   135601 ttatcaaccc tactgtgccg ctatcaatat cttcctgttt gcaacccaaa agtgacagta
   135661 acgcaggggc aattgttaga ccagtaaggc cttttcctga tatacgcaag aattctaatc
   135721 gcgttaagcc aatatctaat agtggacgat caaccgggtt catttatttc gcatcctctt
   135781 cgcaaacaca gtgctgacaa ttcagcagcc tgatattaag cattcaacta atacgctgct
   135841 gactctatcc ccaacaccag atattttccc taacccgata gtgtatgaga aaagatttaa
   135901 cgccgataaa ctaatccttt atggttagga gaaaaaataa tgtggttatt ctgggctctt
   135961 aacattgatc gttatcaatt aaaatt
//
LOCUS       DAZFKF010000002       111488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFKF010000002 DAZFKF010000000
VERSION     DAZFKF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 111488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 111488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..111488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.002"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..961
                     /locus_tag="ACHKA1_000135"
     CDS             <1..961
                     /locus_tag="ACHKA1_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO5677851.1"
                     /translation="KKAREGESAYVLYDDKHKIEGFMYLKENDDANDINPALPPGRHL
                     KIGTFKFESKGTLRGQRFLKKAFDHAFSSKSDDIYVTVFDKHVHLIKLFQTYGFYIHG
                     EKETHNGKEFVYARSLHEPYGDILLDYPRIMTSRANKYLLAIYPEYHTRLFPDSKLVN
                     ESPDIVKDISHANSIHKIYICGMRSVMGMKRGDIIVIYRTGDKKGPARYRSVASTLCV
                     VESVKNISEFLSEDSFVDYCIRFSVFSEDELRKIYKERRYPFIIRFTYNLSLPKRPNR
                     AILIDHVGLNGSRAFRWSHFKLTNEQFLKIIELGKINESFIIH"
     gene            939..1316
                     /locus_tag="ACHKA1_000136"
     CDS             939..1316
                     /locus_tag="ACHKA1_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO5677852.1"
                     /translation="MKVLLSIKPEFAEKILNGTKRFEFRKGIFKNPQISTVVIYATMP
                     LGKVVGQFRIESILSDEPESLWKKTEKHAGISKQFYDSYYSGREKAYAIKIGEVERYK
                     EPIPISALGSNIKPPQSYLYLPA"
     gene            1797..2003
                     /locus_tag="ACHKA1_000137"
     CDS             1797..2003
                     /locus_tag="ACHKA1_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO5677853.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            2078..2374
                     /locus_tag="ACHKA1_000138"
     CDS             2078..2374
                     /locus_tag="ACHKA1_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO5677854.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPLHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            2380..3165
                     /gene="bet"
                     /locus_tag="ACHKA1_000139"
     CDS             2380..3165
                     /gene="bet"
                     /locus_tag="ACHKA1_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO5677855.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            3162..3842
                     /locus_tag="ACHKA1_000140"
     CDS             3162..3842
                     /locus_tag="ACHKA1_000140"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO5677856.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVTPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            3839..3997
                     /locus_tag="ACHKA1_000141"
     CDS             3839..3997
                     /locus_tag="ACHKA1_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HFO5677857.1"
                     /translation="MTHPHDNIRVGAITFVYSITKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKQEDI"
     gene            3994..5058
                     /locus_tag="ACHKA1_000142"
     CDS             3994..5058
                     /locus_tag="ACHKA1_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HFO5677858.1"
                     /translation="MSQVGNHSFEFPASQGVQGGTVTLFLTIPGRSLARFLASDNYGH
                     TLARSQREINPNRVRKFLNYLTNADSRNEPFIIPPLVGNCDSNIEFVPFGNTNVGIAR
                     IPLDAEIKLFDGQHRAAGIEIFCRSSPSTLMVPMMLTMNLPLKTRQQFFSDINNNVSK
                     PSATINMAYNGRDDIAQGMISFLTQHTVFADITDFEHNVVPLKSNMWVSFKALTDATS
                     KFARNGNQQLEMGYIESVWEAWITLTQIDSIRHGVHHATYKRDYIQFHGVMINAFGFA
                     VQQMMVNHSIAEITSMIEKLCATTSSAEREDFFLMDNWAGICTKASQEKLSVIANVAA
                     QKAAANRLIQAFTKGSLETT"
     gene            5212..5433
                     /locus_tag="ACHKA1_000143"
     CDS             5212..5433
                     /locus_tag="ACHKA1_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5677859.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(5644..6246)
                     /locus_tag="ACHKA1_000144"
     CDS             complement(5644..6246)
                     /locus_tag="ACHKA1_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5677860.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            6489..6833
                     /locus_tag="ACHKA1_000145"
     CDS             6489..6833
                     /locus_tag="ACHKA1_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5677861.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            6957..7157
                     /gene="torI"
                     /locus_tag="ACHKA1_000146"
     CDS             6957..7157
                     /gene="torI"
                     /locus_tag="ACHKA1_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFO5677862.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            7401..7574
                     /locus_tag="ACHKA1_000147"
                     /pseudo
     CDS             7401..7574
                     /locus_tag="ACHKA1_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7669..8916)
                     /locus_tag="ACHKA1_000148"
     CDS             complement(7669..8916)
                     /locus_tag="ACHKA1_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO5677863.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(8988..9902)
                     /locus_tag="ACHKA1_000149"
     CDS             complement(8988..9902)
                     /locus_tag="ACHKA1_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO5677864.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDERGMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            10118..11551
                     /gene="cscA"
                     /locus_tag="ACHKA1_000150"
     CDS             10118..11551
                     /gene="cscA"
                     /locus_tag="ACHKA1_000150"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO5677865.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(11559..12554)
                     /locus_tag="ACHKA1_000151"
     CDS             complement(11559..12554)
                     /locus_tag="ACHKA1_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5677866.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGSVTRIPCPLLIRCST"
     gene            12785..13003
                     /locus_tag="ACHKA1_000152"
                     /pseudo
     CDS             12785..13003
                     /locus_tag="ACHKA1_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            13021..14349
                     /gene="dsdA"
                     /locus_tag="ACHKA1_000153"
     CDS             13021..14349
                     /gene="dsdA"
                     /locus_tag="ACHKA1_000153"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO5677867.1"
                     /translation="MENAKMNSLIAQYPLVKVLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELIAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMT
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(14457..15995)
                     /gene="emrY"
                     /locus_tag="ACHKA1_000154"
     CDS             complement(14457..15995)
                     /gene="emrY"
                     /locus_tag="ACHKA1_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO5677868.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(15995..17158)
                     /gene="emrK"
                     /locus_tag="ACHKA1_000155"
     CDS             complement(15995..17158)
                     /gene="emrK"
                     /locus_tag="ACHKA1_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO5677869.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            17574..18188
                     /gene="evgA"
                     /locus_tag="ACHKA1_000156"
     CDS             17574..18188
                     /gene="evgA"
                     /locus_tag="ACHKA1_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO5677870.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            18193..21786
                     /gene="evgS"
                     /locus_tag="ACHKA1_000157"
     CDS             18193..21786
                     /gene="evgS"
                     /locus_tag="ACHKA1_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO5677871.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAEQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADFYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVVHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQQND"
     gene            complement(21842..22987)
                     /gene="yfdE"
                     /locus_tag="ACHKA1_000158"
     CDS             complement(21842..22987)
                     /gene="yfdE"
                     /locus_tag="ACHKA1_000158"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO5677872.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(23061..24005)
                     /gene="yfdV"
                     /locus_tag="ACHKA1_000159"
     CDS             complement(23061..24005)
                     /gene="yfdV"
                     /locus_tag="ACHKA1_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO5677873.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(24075..25769)
                     /gene="oxc"
                     /locus_tag="ACHKA1_000160"
     CDS             complement(24075..25769)
                     /gene="oxc"
                     /locus_tag="ACHKA1_000160"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO5677874.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(25823..27073)
                     /gene="frc"
                     /locus_tag="ACHKA1_000161"
     CDS             complement(25823..27073)
                     /gene="frc"
                     /locus_tag="ACHKA1_000161"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO5677875.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISIDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSDFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(27585..28199)
                     /gene="yfdX"
                     /locus_tag="ACHKA1_000162"
     CDS             complement(27585..28199)
                     /gene="yfdX"
                     /locus_tag="ACHKA1_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO5677876.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQKTAAAERISE
                     QGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNVNDDQ
                     YIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQYLMP
                     LKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            28495..28770
                     /gene="ypdI"
                     /locus_tag="ACHKA1_000163"
     CDS             28495..28770
                     /gene="ypdI"
                     /locus_tag="ACHKA1_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO5677877.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSNSGGGSETSYKEPEKTSAM
                     TTTHSTKFQPSQAILFKMREDAPPLNLTEETTPPFPTKASYLIHPVR"
     gene            complement(28847..29089)
                     /gene="yfdY"
                     /locus_tag="ACHKA1_000164"
     CDS             complement(28847..29089)
                     /gene="yfdY"
                     /locus_tag="ACHKA1_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO5677878.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            29442..30362
                     /gene="lpxP"
                     /locus_tag="ACHKA1_000165"
     CDS             29442..30362
                     /gene="lpxP"
                     /locus_tag="ACHKA1_000165"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO5677879.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            30718..30789
                     /gene="ypdK"
                     /locus_tag="ACHKA1_000166"
     CDS             30718..30789
                     /gene="ypdK"
                     /locus_tag="ACHKA1_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO5677880.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(30854..32092)
                     /gene="alaC"
                     /locus_tag="ACHKA1_000167"
     CDS             complement(30854..32092)
                     /gene="alaC"
                     /locus_tag="ACHKA1_000167"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO5677881.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVSGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            32469..34166
                     /gene="ypdA"
                     /locus_tag="ACHKA1_000168"
     CDS             32469..34166
                     /gene="ypdA"
                     /locus_tag="ACHKA1_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO5677882.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            34181..34915
                     /gene="ypdB"
                     /locus_tag="ACHKA1_000169"
     CDS             34181..34915
                     /gene="ypdB"
                     /locus_tag="ACHKA1_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO5677883.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            34928..35785
                     /gene="ypdC"
                     /locus_tag="ACHKA1_000170"
     CDS             34928..35785
                     /gene="ypdC"
                     /locus_tag="ACHKA1_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5677884.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRENVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(35788..38283)
                     /gene="ptsP"
                     /locus_tag="ACHKA1_000171"
     CDS             complement(35788..38283)
                     /gene="ptsP"
                     /locus_tag="ACHKA1_000171"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5677885.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGEIRYLPLL
                     LGLDLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVIGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(38308..39345)
                     /gene="ypdE"
                     /locus_tag="ACHKA1_000172"
     CDS             complement(38308..39345)
                     /gene="ypdE"
                     /locus_tag="ACHKA1_000172"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5677886.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(39345..40430)
                     /gene="ypdF"
                     /locus_tag="ACHKA1_000173"
     CDS             complement(39345..40430)
                     /gene="ypdF"
                     /locus_tag="ACHKA1_000173"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5677887.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLATIVNEIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKTSFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSTESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDEAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(40445..41692)
                     /gene="fryC"
                     /locus_tag="ACHKA1_000174"
     CDS             complement(40445..41692)
                     /gene="fryC"
                     /locus_tag="ACHKA1_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO5677888.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(41714..42040)
                     /gene="fryB"
                     /locus_tag="ACHKA1_000175"
     CDS             complement(41714..42040)
                     /gene="fryB"
                     /locus_tag="ACHKA1_000175"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO5677889.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(42259..43224)
                     /gene="glk"
                     /locus_tag="ACHKA1_000176"
     CDS             complement(42259..43224)
                     /gene="glk"
                     /locus_tag="ACHKA1_000176"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO5677890.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPIEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            43428..44685
                     /gene="yfeO"
                     /locus_tag="ACHKA1_000177"
                     /pseudo
     CDS             43428..44685
                     /gene="yfeO"
                     /locus_tag="ACHKA1_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
     gene            44800..45126
                     /gene="ypeC"
                     /locus_tag="ACHKA1_000178"
     CDS             44800..45126
                     /gene="ypeC"
                     /locus_tag="ACHKA1_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO5677891.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(45267..46505)
                     /gene="mntH"
                     /locus_tag="ACHKA1_000179"
     CDS             complement(45267..46505)
                     /gene="mntH"
                     /locus_tag="ACHKA1_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO5677892.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            46841..48043
                     /gene="nupC"
                     /locus_tag="ACHKA1_000180"
     CDS             46841..48043
                     /gene="nupC"
                     /locus_tag="ACHKA1_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO5677893.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(48093..50282)
                     /gene="pdeA"
                     /locus_tag="ACHKA1_000181"
     CDS             complement(48093..50282)
                     /gene="pdeA"
                     /locus_tag="ACHKA1_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO5677894.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(50490..50565)
                     /locus_tag="ACHKA1_000182"
     tRNA            complement(50490..50565)
                     /locus_tag="ACHKA1_000182"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50530..50532),aa:Ala,seq:ggc)
     gene            complement(50605..50680)
                     /locus_tag="ACHKA1_000183"
     tRNA            complement(50605..50680)
                     /locus_tag="ACHKA1_000183"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50645..50647),aa:Ala,seq:ggc)
     gene            50901..51260
                     /gene="yfeC"
                     /locus_tag="ACHKA1_000184"
     CDS             50901..51260
                     /gene="yfeC"
                     /locus_tag="ACHKA1_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO5677895.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            51262..51654
                     /gene="yfeD"
                     /locus_tag="ACHKA1_000185"
     CDS             51262..51654
                     /gene="yfeD"
                     /locus_tag="ACHKA1_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO5677896.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(51706..53121)
                     /gene="gltX"
                     /locus_tag="ACHKA1_000186"
     CDS             complement(51706..53121)
                     /gene="gltX"
                     /locus_tag="ACHKA1_000186"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO5677897.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            53380..53455
                     /locus_tag="ACHKA1_000187"
     tRNA            53380..53455
                     /locus_tag="ACHKA1_000187"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53413..53415,aa:Val,seq:tac)
     gene            53500..53575
                     /locus_tag="ACHKA1_000188"
     tRNA            53500..53575
                     /locus_tag="ACHKA1_000188"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53533..53535,aa:Val,seq:tac)
     gene            53580..53655
                     /locus_tag="ACHKA1_000189"
     tRNA            53580..53655
                     /locus_tag="ACHKA1_000189"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53613..53615,aa:Lys,seq:ttt)
     gene            53686..53811
                     /locus_tag="ACHKA1_000190"
     ncRNA           53686..53811
                     /ncRNA_class="other"
                     /locus_tag="ACHKA1_000190"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            53931..54272
                     /locus_tag="ACHKA1_000191"
     CDS             53931..54272
                     /locus_tag="ACHKA1_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO5677898.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(54263..55189)
                     /gene="yfeR"
                     /locus_tag="ACHKA1_000192"
     CDS             complement(54263..55189)
                     /gene="yfeR"
                     /locus_tag="ACHKA1_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5677899.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            55279..56277
                     /gene="yfeH"
                     /locus_tag="ACHKA1_000193"
     CDS             55279..56277
                     /gene="yfeH"
                     /locus_tag="ACHKA1_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO5677900.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            complement(56274..56492)
                     /gene="ypeB"
                     /locus_tag="ACHKA1_000194"
     CDS             complement(56274..56492)
                     /gene="ypeB"
                     /locus_tag="ACHKA1_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO5677901.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(56494..58509)
                     /gene="ligA"
                     /locus_tag="ACHKA1_000195"
     CDS             complement(56494..58509)
                     /gene="ligA"
                     /locus_tag="ACHKA1_000195"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO5677902.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(58580..59578)
                     /gene="zipA"
                     /locus_tag="ACHKA1_000196"
     CDS             complement(58580..59578)
                     /gene="zipA"
                     /locus_tag="ACHKA1_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO5677903.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            59808..60569
                     /gene="cysZ"
                     /locus_tag="ACHKA1_000197"
     CDS             59808..60569
                     /gene="cysZ"
                     /locus_tag="ACHKA1_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO5677904.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            60754..61725
                     /gene="cysK"
                     /locus_tag="ACHKA1_000198"
     CDS             60754..61725
                     /gene="cysK"
                     /locus_tag="ACHKA1_000198"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO5677905.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            62109..62366
                     /gene="ptsH"
                     /locus_tag="ACHKA1_000199"
     CDS             62109..62366
                     /gene="ptsH"
                     /locus_tag="ACHKA1_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO5677906.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            62411..64138
                     /gene="ptsI"
                     /locus_tag="ACHKA1_000200"
     CDS             62411..64138
                     /gene="ptsI"
                     /locus_tag="ACHKA1_000200"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO5677907.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            64179..64688
                     /gene="crr"
                     /locus_tag="ACHKA1_000201"
     CDS             64179..64688
                     /gene="crr"
                     /locus_tag="ACHKA1_000201"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO5677908.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(64731..65582)
                     /gene="pdxK"
                     /locus_tag="ACHKA1_000202"
                     /gene_synonym="thiJ"
     CDS             complement(64731..65582)
                     /gene="pdxK"
                     /locus_tag="ACHKA1_000202"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO5677909.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLVEA"
     gene            65687..66055
                     /gene="yfeK"
                     /locus_tag="ACHKA1_000203"
     CDS             65687..66055
                     /gene="yfeK"
                     /locus_tag="ACHKA1_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO5677910.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(66058..66969)
                     /gene="cysM"
                     /locus_tag="ACHKA1_000204"
     CDS             complement(66058..66969)
                     /gene="cysM"
                     /locus_tag="ACHKA1_000204"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO5677911.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMADRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPTEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQRAGI"
     gene            complement(67089..68186)
                     /gene="cysA"
                     /locus_tag="ACHKA1_000205"
     CDS             complement(67089..68186)
                     /gene="cysA"
                     /locus_tag="ACHKA1_000205"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO5677912.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDNAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(68176..69051)
                     /gene="cysW"
                     /locus_tag="ACHKA1_000206"
     CDS             complement(68176..69051)
                     /gene="cysW"
                     /locus_tag="ACHKA1_000206"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO5677913.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEENHEH"
     gene            complement(69051..69884)
                     /gene="cysT"
                     /locus_tag="ACHKA1_000207"
     CDS             complement(69051..69884)
                     /gene="cysT"
                     /locus_tag="ACHKA1_000207"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO5677914.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(69884..70900)
                     /gene="cysP"
                     /locus_tag="ACHKA1_000208"
     CDS             complement(69884..70900)
                     /gene="cysP"
                     /locus_tag="ACHKA1_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO5677915.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(71204..71995)
                     /gene="ucpA"
                     /locus_tag="ACHKA1_000209"
     CDS             complement(71204..71995)
                     /gene="ucpA"
                     /locus_tag="ACHKA1_000209"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO5677916.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPVSVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(72124..72981)
                     /gene="murR"
                     /locus_tag="ACHKA1_000210"
     CDS             complement(72124..72981)
                     /gene="murR"
                     /locus_tag="ACHKA1_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO5677917.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKQLALEQTCALFDFARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHYTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            73145..74041
                     /gene="murQ"
                     /locus_tag="ACHKA1_000211"
     CDS             73145..74041
                     /gene="murQ"
                     /locus_tag="ACHKA1_000211"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO5677918.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISSNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRKVLDKE"
     gene            74045..75469
                     /gene="murP"
                     /locus_tag="ACHKA1_000212"
     CDS             74045..75469
                     /gene="murP"
                     /locus_tag="ACHKA1_000212"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO5677919.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSSQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            75474..76778
                     /gene="pbp4b"
                     /locus_tag="ACHKA1_000213"
     CDS             75474..76778
                     /gene="pbp4b"
                     /locus_tag="ACHKA1_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO5677920.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(76836..77735)
                     /gene="yfeX"
                     /locus_tag="ACHKA1_000214"
     CDS             complement(76836..77735)
                     /gene="yfeX"
                     /locus_tag="ACHKA1_000214"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO5677921.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(77831..78406)
                     /gene="yfeY"
                     /locus_tag="ACHKA1_000215"
     CDS             complement(77831..78406)
                     /gene="yfeY"
                     /locus_tag="ACHKA1_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO5677922.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(78467..78916)
                     /gene="yfeZ"
                     /locus_tag="ACHKA1_000216"
     CDS             complement(78467..78916)
                     /gene="yfeZ"
                     /locus_tag="ACHKA1_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO5677923.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGFGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(78903..79328)
                     /gene="ypeA"
                     /locus_tag="ACHKA1_000217"
     CDS             complement(78903..79328)
                     /gene="ypeA"
                     /locus_tag="ACHKA1_000217"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO5677924.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            79542..80411
                     /gene="amiA"
                     /locus_tag="ACHKA1_000218"
     CDS             79542..80411
                     /gene="amiA"
                     /locus_tag="ACHKA1_000218"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO5677925.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            80415..81314
                     /gene="hemF"
                     /locus_tag="ACHKA1_000219"
     CDS             80415..81314
                     /gene="hemF"
                     /locus_tag="ACHKA1_000219"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO5677926.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGTEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRKEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(81320..82372)
                     /gene="eutR"
                     /locus_tag="ACHKA1_000220"
     CDS             complement(81320..82372)
                     /gene="eutR"
                     /locus_tag="ACHKA1_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO5677927.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(82418..82918)
                     /gene="eutK"
                     /locus_tag="ACHKA1_000221"
     CDS             complement(82418..82918)
                     /gene="eutK"
                     /locus_tag="ACHKA1_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO5677928.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(82931..83590)
                     /gene="eutL"
                     /locus_tag="ACHKA1_000222"
     CDS             complement(82931..83590)
                     /gene="eutL"
                     /locus_tag="ACHKA1_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO5677929.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(83600..84487)
                     /gene="eutC"
                     /locus_tag="ACHKA1_000223"
     CDS             complement(83600..84487)
                     /gene="eutC"
                     /locus_tag="ACHKA1_000223"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO5677930.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCTTTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(84508..85869)
                     /gene="eutB"
                     /locus_tag="ACHKA1_000224"
     CDS             complement(84508..85869)
                     /gene="eutB"
                     /locus_tag="ACHKA1_000224"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO5677931.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(85881..87284)
                     /gene="eutA"
                     /locus_tag="ACHKA1_000225"
     CDS             complement(85881..87284)
                     /gene="eutA"
                     /locus_tag="ACHKA1_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO5677932.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(87281..88507)
                     /gene="eutH"
                     /locus_tag="ACHKA1_000226"
     CDS             complement(87281..88507)
                     /gene="eutH"
                     /locus_tag="ACHKA1_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO5677933.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHILGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            complement(88724..89911)
                     /gene="eutG"
                     /locus_tag="ACHKA1_000227"
     CDS             complement(88724..89911)
                     /gene="eutG"
                     /locus_tag="ACHKA1_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO5677934.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVCLYA
                     AAQ"
     gene            complement(89901..90737)
                     /gene="eutJ"
                     /locus_tag="ACHKA1_000228"
     CDS             complement(89901..90737)
                     /gene="eutJ"
                     /locus_tag="ACHKA1_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO5677935.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRIPLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(90748..92151)
                     /gene="eutE"
                     /locus_tag="ACHKA1_000229"
     CDS             complement(90748..92151)
                     /gene="eutE"
                     /locus_tag="ACHKA1_000229"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO5677936.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(92163..92450)
                     /gene="eutN"
                     /locus_tag="ACHKA1_000230"
     CDS             complement(92163..92450)
                     /gene="eutN"
                     /locus_tag="ACHKA1_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO5677937.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPHGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(92557..92850)
                     /gene="eutM"
                     /locus_tag="ACHKA1_000231"
     CDS             complement(92557..92850)
                     /gene="eutM"
                     /locus_tag="ACHKA1_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO5677938.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(92889..93905)
                     /gene="pta"
                     /locus_tag="ACHKA1_000232"
     CDS             complement(92889..93905)
                     /gene="pta"
                     /locus_tag="ACHKA1_000232"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO5677939.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTE
                     PLMFAAAMVSAGIADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(93902..94705)
                     /gene="eutT"
                     /locus_tag="ACHKA1_000233"
     CDS             complement(93902..94705)
                     /gene="eutT"
                     /locus_tag="ACHKA1_000233"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO5677940.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLGHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(94702..95403)
                     /gene="eutQ"
                     /locus_tag="ACHKA1_000234"
     CDS             complement(94702..95403)
                     /gene="eutQ"
                     /locus_tag="ACHKA1_000234"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO5677941.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESSPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWDNAFFPWTLNYDEIDMVLEGELHVRHAGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(95378..95857)
                     /gene="eutP"
                     /locus_tag="ACHKA1_000235"
     CDS             complement(95378..95857)
                     /gene="eutP"
                     /locus_tag="ACHKA1_000235"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO5677942.1"
                     /translation="MKRIAFVGTVGAGKTTLFNVLQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(95870..96205)
                     /gene="eutS"
                     /locus_tag="ACHKA1_000236"
     CDS             complement(95870..96205)
                     /gene="eutS"
                     /locus_tag="ACHKA1_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO5677943.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(96498..98777)
                     /gene="maeB"
                     /locus_tag="ACHKA1_000237"
     CDS             complement(96498..98777)
                     /gene="maeB"
                     /locus_tag="ACHKA1_000237"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO5677944.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            99066..100016
                     /gene="tal"
                     /locus_tag="ACHKA1_000238"
     CDS             99066..100016
                     /gene="tal"
                     /locus_tag="ACHKA1_000238"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO5677945.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            100036..102039
                     /gene="tkt"
                     /locus_tag="ACHKA1_000239"
     CDS             100036..102039
                     /gene="tkt"
                     /locus_tag="ACHKA1_000239"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO5677946.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(102134..103177)
                     /gene="ypfG"
                     /locus_tag="ACHKA1_000240"
     CDS             complement(102134..103177)
                     /gene="ypfG"
                     /locus_tag="ACHKA1_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO5677947.1"
                     /translation="MRYRNLLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(103303..103878)
                     /gene="nudK"
                     /locus_tag="ACHKA1_000241"
     CDS             complement(103303..103878)
                     /gene="nudK"
                     /locus_tag="ACHKA1_000241"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO5677948.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(103946..105925)
                     /gene="aegA"
                     /locus_tag="ACHKA1_000242"
     CDS             complement(103946..105925)
                     /gene="aegA"
                     /locus_tag="ACHKA1_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO5677949.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            106131..107831
                     /gene="narQ"
                     /locus_tag="ACHKA1_000243"
     CDS             106131..107831
                     /gene="narQ"
                     /locus_tag="ACHKA1_000243"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO5677950.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            107995..111108
                     /gene="acrD"
                     /locus_tag="ACHKA1_000244"
     CDS             107995..111108
                     /gene="acrD"
                     /locus_tag="ACHKA1_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO5677951.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(111207..111266)
                     /gene="ypfM"
                     /locus_tag="ACHKA1_000245"
     CDS             complement(111207..111266)
                     /gene="ypfM"
                     /locus_tag="ACHKA1_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO5677952.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        28012 a        29238 c        27217 g        27021 t
ORIGIN      
        1 aaaaaaagcc agagaaggag aatcagctta tgtgctctat gatgacaagc ataaaatcga
       61 aggttttatg tatctaaaag aaaatgatga tgcaaatgac attaatccag cgctcccacc
      121 aggacgtcat ctaaagatag gaacattcaa atttgaatct aaaggcaccc ttcgcggaca
      181 acgatttcta aaaaaagcgt ttgatcatgc attttcatca aaatctgatg atatttatgt
      241 tactgttttc gacaaacacg tccatctaat aaaacttttc caaacgtacg gattttacat
      301 tcatggtgaa aaagaaacac ataacgggaa agagtttgta tatgcgaggt ctttgcatga
      361 gccttatggt gatattttat tagattaccc tcgaataatg acatcaaggg ccaacaaata
      421 tttactggcg atttatcccg aatatcacac tagactattc cctgattcaa aacttgtaaa
      481 tgaatcacca gatattgtca aagatatatc ccatgctaac agcattcata aaatttacat
      541 atgtggaatg cgttctgtga tgggaatgaa aagaggagat atcattgtca tctatagaac
      601 cggagacaaa aaagggccag ctcgctatcg ttctgtagcc agtacattat gtgtagttga
      661 gagcgtaaaa aatatttctg aatttttaag cgaagatagt tttgtagact attgtattcg
      721 ttttagcgta ttttctgaag atgaactcag aaaaatctat aaagaacgtc gatacccttt
      781 cattataaga ttcacataca atctgtcttt gccaaagaga cccaatcgtg ctattttaat
      841 agatcatgtg gggctaaatg gttcgcgtgc attccgatgg agtcacttta aactcacaaa
      901 tgagcagttc ttaaagatca tcgagttagg caagataaat gaaagtttta ttatccatta
      961 agcctgagtt tgcagaaaaa atattgaacg gaacaaagcg gttcgagttt cgtaaaggta
     1021 tattcaaaaa tccgcaaatt agcaccgttg ttatttatgc cacgatgcca ttaggtaaag
     1081 ttgttggtca attccgtatt gaatcaatac taagtgacga accggaatct ctttggaaaa
     1141 agacggaaaa acacgcaggt atttctaagc aattttatga ctcatattat tcaggtagag
     1201 aaaaggccta cgcaataaaa attggtgaag tggaaagata taaagaacca attcctatct
     1261 ctgctctagg tagtaatatt aagccaccac aatcatatct ttacctacct gcgtaagaat
     1321 cccggccacc gtgccgggtt ttcttttgtc ccctcccctc atcacacaaa ccgttcgaaa
     1381 aaccaccaca acctcccttc agttatcgct atgcgatgca agtcacaaaa ttaattcttt
     1441 ttgctatcaa acagttaata tcaaaacaca tcaatcaata tcaataagta ttgatgcaac
     1501 caatagcaat agctattatc accatgtcgc aacaacacaa cgatacggca atcacctgat
     1561 tcaccgttgc gatgaccgct tagatccgca gcttgaattt cagcaggctc cggggagtgc
     1621 gaggggtgaa gcggacgcgt gaacgtcggt gtgaccagct gaaatcaact caacacctca
     1681 tacctcagtc gcttcaacga ggcggcttag ttatgacaac cggcggccat ccaccgcctg
     1741 aatacgcgca gaagtctcta tatgttcagc agcccagctt acgggcagga gtttttatgg
     1801 ttcatcaaca ttacggaacg cagaccgtta atcgcggtgc ggtcatgcca ggaatgctgg
     1861 tcaaacacaa agatggtacc tggactgcat cagctaattt acgcggacgg ctttatctgc
     1921 atcgcggcat cgagcgcact tatacccgtg atttgctcgt ggaagttttt ctcgacggac
     1981 gcggcaacgg cctgaatcac taatcccctt tcctgtttac ctaatcagcc cggcatttcg
     2041 caggcgatat tttcacagct atttcaggag ttcagccatg aacgcttatt acattcagga
     2101 tcgtcttgag gctcagagct gggcgcgtca ctaccagcag atcgcccgtg aagagaaaga
     2161 ggcagaactg gcagacgaca tggaaaaagg cctgcccctg cacctgtttg aatcgctatg
     2221 catcgatcat ttgcaacgcc acggggccag caaaaaagcc attacccgtg cgtttgatga
     2281 cgatgttgag tttcaggagc gcatggcaga acacatccgg tacatggttg aaaccattgc
     2341 tcaccaccag gttgatattg attcagaggt ataaaacgga tgagtacagc actcgcaacg
     2401 ctggctggga agctggctga acgtgtcggc atggattctg tcgacccaca ggaactgatc
     2461 accactcttc gccagacagc atttaaaggc gatgccagcg atgcgcagtt catcgcattg
     2521 ctgatcgtcg ccaaccaata cggccttaat ccgtggacga aagaaattta cgccttccct
     2581 gataagcaga acggcatcgt tccggtggtg ggcgttgatg gctggtcccg catcatcaac
     2641 gaaaaccagc agtttgacgg catggacttt gaacaggaca acgaatcctg cacatgccgg
     2701 atttaccgca aagaccgcaa tcatccgatc tgcgttaccg agtggatgga tgaatgccgc
     2761 cgcgaaccat tcaaaacccg cgaaggcaga gaaatcacgg ggccgtggca gtcgcatccc
     2821 aaacggatgt tacggcataa agccatgatt cagtgtgccc gtctcgcctt cggatttgct
     2881 ggtatctatg acaaggatga agccgagcgc attgtcgaaa ataccgcata cactgcagaa
     2941 cgtcagccgg aacgcgacat cactccggtt aacgatgaaa ccatgcagga gattaacact
     3001 ctgctgatcg ccctggataa aacatgggat gacgacttat tgccgctctg ttcccagata
     3061 tttcgccgcg acattcgcgc atcgtcagaa ctgacacagg ccgaagcagt gaaagctctt
     3121 ggattcctga aacagaaagc cactgagcag aaggtggcag catgacaccg gacattatcc
     3181 tgcagcgtac cgggatcgac gtgagagctg tcgaacaggg ggatgatgcg tggcacaaat
     3241 tacggctcgg cgtcatcacc gcttcagaag ttcacaacgt gatagcaaag ccccgctcag
     3301 gaaagaagtg gcctgacatg aaaatgtcct acttccacac cctgctggct gaggtttgca
     3361 ccggtgtgac tccggaagtt aatgctaagg cgctggcctg gggaaaacag tacgagaacg
     3421 acgccagaac tctgtttgaa ttcacttccg gcgtgaatgt tactgaatcc ccgatcatct
     3481 atcgcgacga aagtatgcgc accgcctgct ctcccgatgg tttatgcagt gacggcaatg
     3541 gccttgagct gaaatgcccg tttacctccc gggatttcat gaagttccgg ctcggtggtt
     3601 tcgaggccat aaagtcggct tacatggccc aggtgcagta cagcatgtgg gtgacacgaa
     3661 aagatgcctg gtactttgcc aactatgacc cgcgtatgaa gcgtgaagga ctgcattatg
     3721 tcgtggttga gcgggatgaa aagtacatgg cgagttttga cgagatggtg ccggagttca
     3781 tcgaaaaaat ggacgaggca ctggctgaaa ttggttttgt atttggggag caatggcgat
     3841 gacgcatcct cacgataata tccgggtagg cgcgatcact ttcgtctact ccattacaaa
     3901 gcgaggctgg gtatttcccg gcctttctgt tatcagaaat ccactgaaag cacagcggct
     3961 ggctgagaag ataaataata aacaggagga tatatgagtc aggttggtaa tcattcattc
     4021 gaatttccgg catcgcaagg tgtacagggt ggtactgtta cactcttcct taccatacca
     4081 ggaagatcgc tggctcgttt cctcgcttca gataattacg gccatacact ggcacgctct
     4141 cagcgagaaa ttaatccaaa tcgagtacga aaatttttaa attatctcac taacgcagac
     4201 tcaagaaatg agccttttat cattccccct ctcgtaggta actgtgattc gaatatagaa
     4261 tttgtaccgt ttggcaacac aaatgttggt atagccagaa ttcccctcga cgccgaaata
     4321 aaactttttg atggtcaaca tcgtgcagct ggcattgaga tattttgccg aagttcccca
     4381 tcaacgctca tggttcccat gatgcttaca atgaatctgc cgctaaaaac ccggcagcag
     4441 ttcttttcgg acataaataa caacgtttct aagccatcag cgaccatcaa tatggcgtat
     4501 aacggccggg atgatattgc tcagggaatg atatccttcc tgacccaaca tactgtattt
     4561 gccgatataa ccgattttga acacaacgta gtgccattaa aaagtaatat gtgggtgagt
     4621 ttcaaggcac tcactgatgc aacgtcaaag ttcgctagga acggcaatca acaacttgaa
     4681 atgggatata tagaatctgt ctgggaggca tggattacac taactcagat tgactcaatc
     4741 cgacatggtg tacaccacgc tacgtacaag cgcgattata ttcagttcca tggagtaatg
     4801 attaacgctt tcggttttgc ggttcaacag atgatggtta atcattccat cgcagaaata
     4861 acttctatga tcgaaaaact atgtgcaact accagctctg cagaaagaga ggattttttt
     4921 ctgatggata actgggcggg gatctgcacg aaagccagcc aggaaaaact atctgttatt
     4981 gccaatgtgg cagcgcagaa agcagcagca aacagactga tacaagcttt taccaaagga
     5041 agtctggaaa caacttaatg aatcaacatt gtctcatatc agcatgctgt acggcgtctt
     5101 taaggaacgg tgagcatgaa aaacaaaatc atcatggagc tacaggctcc ttttttatta
     5161 ttcgcattca ccctcaagcg tattaaccaa caattcaggg attaatgaaa gatggcagac
     5221 atcattgatt cagcatcaga aattgaagaa ttacagcgca acacagcaat aaaaatgcgc
     5281 cgcctgaacc accaggctat atctgccact cattgttgtg agtgtggcga tccgatagat
     5341 gaacgaagac gcctggccgt tcagggttgt cggacttgtg caagttgcca ggaggatctg
     5401 gaacttatca gtaaacagag aggttcgaag tgagcgaaat taactagaag ccaaagataa
     5461 aatcatcgct gagcaggaga aaatcgctaa cggagaaaag acagtaagtc agtatatgaa
     5521 aaccgcatga tatcatcaga taaaaaccga tcgtaaagcg aaatattaat accagaataa
     5581 acgagtcgag gtaaattata ttacctcgat aaattaacta aaacttgccc gctatatact
     5641 atctcattca gtatcatcac gcgcggtctg tgcatatgtc actaccgcac ctaatgtatt
     5701 aattttcttt tcaacataga taatattatc gtactcataa ttgccatacg gatagcaaat
     5761 gcgaatattc tcatgtagat cggggtcatc cacctcagct ccagaacaac tttttgaact
     5821 accggaagta taccgatacg gtgcaacata agacgatgtc tctccaggca aaaaataagt
     5881 tagtgttgta aggggtataa tcagaaaaaa tccagaaaat atgcacatcc ctgcataaac
     5941 cttaaggtat gctgacagac tcttccagcc gctttgtttt actatcccct tcttaaccca
     6001 aaacagagat aacagaaaag ctattcccat gctaaacaga atgtaatagt gggatatact
     6061 ctgattaaga aacgtgaccc tgtagatatc tgcccgccac cagaagaaaa ggaaaataaa
     6121 gatcagccct gaaactgtca tgcaaatcaa ataaggatac gaatcttttt tcatgtttag
     6181 cgcccataaa atttttcctg caccggacaa atttaccatc cattttttgc gcagaaaata
     6241 gctcattact tactgcacaa taatacacaa aattgcgtaa attttttgca tggattttag
     6301 ctctttcagc cgacgtttaa ggggtaaata gcatttccta aaagcaactg caccaaccca
     6361 acagaatggg ctaccgctta cgttgagagc aaaaaagtgt atagcagcaa tgaacagcat
     6421 cctcgcactg acggggattt cttttatctg agctcgctac ggcgagtttt gttttatgga
     6481 gacaagaaat atcagatttg gctatgaagg ttttgaaatg gcaatcaact ggcgatgtcg
     6541 gcatcagtag cgcaactctt gcctcaatcg catgtggcct gaaaaagaat atctatggtc
     6601 atcacttcgg cgctccccat gacgcagcag actttcggcg atgcgttgca cttgttgagc
     6661 agattccaga aatcagagat tcattcgaca aggttgcaaa gcgcgttccg gcattcaaag
     6721 gaatcctcaa cgaatgggat tcactcgttg ctctgttgaa gtctgaaatg aagacgtacg
     6781 ggaacaaagc accagagact tacagaagaa tcagcgagct acgcaaggac taacccgcct
     6841 cacactcgat gaggcatttt catttatcaa gatatccaga cctaccatcg ccgcatcaat
     6901 gcggtttttt tattacctga tttgcaggtt cgattcccta ttcggagata gcactcatgc
     6961 aacacgaact acagcctgat tccctggttg atttgaaatt catcatggcc gatactggct
     7021 tcggtaaaac cttcatctat gaccggatta agtccggaga cctgcctaaa gccaaagtta
     7081 tccacgggcg agcaagatgg ttatatcgtg accattgtga attcaaaaat aagctcttaa
     7141 gccgcgccaa tgggtaaaat agcgggtaaa atatttctca catctaaaaa acaccattct
     7201 aatcaacccc ctgccgcctc aagtagatgt ctgcagggga caccagatac ccttcaaacg
     7261 aaatctacct tcaccccgta aaagatgggt ttggcagcac acttgcccta tatctactca
     7321 tttttactgc aacaggttga aatctcagca ctgtcagaaa gcgctgatga ctaaacagcc
     7381 ttgggccggg cgatgtaacc atcacacaga atcctgatag cgaaatatgg cgtgactcga
     7441 tacttcactc cgcaatgcat tccttgatga attcgcaggc ccgtgataca cgggacaggt
     7501 cgctgaatga cgacaatgtc ctggaaatca gcgaaccgcg tatccgaagt acatttgagc
     7561 gactgtacca gaacataggt gattattagc agggctaagc attttagtat tattattttc
     7621 cggttgaggg atatggagat atcgacaaca accggaaaaa gtttacgtct atattgctga
     7681 aggtacaggc gtttccataa ctatttgctc gcgtttttta ctcaggaaga aaatgccaaa
     7741 tagcaacatc aggcagacaa tacccgaaat tgcgaagaaa actgtctggt agcctgcgtg
     7801 gtcaaagagt atcccagtcg gcgttgaaag cagcacaatc ccaagcgaac tggcaatttg
     7861 aaaaccaatc agaaagatcg tcgacgacag gcgcttatca aagtttgcca cgctgtattt
     7921 gaagacggat atgacacaaa gtggaacctc aatagcatgt aacagcttca ctaatgaaat
     7981 aatccagggg ttaacgaaca gcgcgcagga aaggatacgc aacgccataa tcacaacacc
     8041 gataagtaat gcattttttg gccctacccg attcacaaag aaaggaataa tcgccatgca
     8101 cagcgcttcg agtaccacct ggaatgagtt gagataacca tacaggcgcg ttcctacatc
     8161 gtgtgattcg aataaacctg cataaaagac aggaaaaagt tgttgatcaa aaatgttata
     8221 gaaagaccac gtccccacaa taaatatgac gaaaacccag aagtttcgat ccttgaaaac
     8281 tgcgataaaa tcctcttttt ttacccctcc cgcatccgcc gctacgcact ggtgatcctt
     8341 atctttaaaa cacatgttga tcatcataaa tacagcgcca aatagcgaga ccaaccagaa
     8401 gttgatatgg ggactgatac taaaaaatat gccggcaaag aacgcgccaa tagcatagcc
     8461 aaaagatccc caggcgcgcg ctgttccata ttcgaaatga aaatttcgcg ccattttttc
     8521 ggtgaagctg tcaagcaaac cgcatcccgc cagatacccc aagccaaaaa agagcgcccc
     8581 cagaattaga cctacagaaa aattgctttg cagtaacggt tcataaacgt aaatcataaa
     8641 cggtccggtc aagaccagga tgaaactcat acaccagatg agtggtttct tcagaccgag
     8701 tttatcctga acgatgccgt agaacatcat aaatagaatg ctggtaaact ggttgaccga
     8761 ataaagtgta cctaattccg tccctgtcaa ccctagatgt cctttcagcc aaatagcgta
     8821 taacgaccac cacagcgacc aggaaataaa aaagagaaat gagtaactgg atgcaaaacg
     8881 atagtacgca tttctgaatg ggatattcag tgccataatt acctgcctgt cgttaaaaaa
     8941 ttcacgtcct atttagagat aagagcgact tcgccgttta cttctcacta ttccagttct
     9001 tgtcgacatg gcagcgctgt cattgcccct ttcgccgtta ctgcaagcgc tccgcaacgt
     9061 tgagcgagat cgataattcg tcgcattcct ctctcatctg tagataatcc cgtagaggac
     9121 agacctgtga gtaacccggc aacgaacgca tctcccgccc ccgtgctatc gacacaattc
     9181 acagacattc cagcaaaatg gtgaacttgt cctcgataac agaccaccac cccttctgca
     9241 cctttagtca ccaacagcat ggcgatctca tactcttttg ccagggcgca tatatcccga
     9301 tcgttctgtg tttttccact gattagtcgc cattcttctt ccgagagctt gacgacatcc
     9361 gccagttgta gcgcctgccg caaacacaag cggagcaaat gctcgtcttg ccatagatct
     9421 tcacgaatat tgggatcgaa gctgacaaaa cctccggcat gccggatcgc cgtcatcgca
     9481 gtaaatgcgc tggtacgcga aggctcggca gacaacgcaa ttgaacagag atgtaaccat
     9541 tcgccatgtc gccagcaggg caagtctgtc gtctctaaaa aaagatcggc actggggcga
     9601 accataaacg taaatgaacg ttccccttga tcgttcagat cgacaagcac cgtggatgtc
     9661 cggtgccatt catcttgctt cagatacgtg atatcgactc cctcagttag cagagttctt
     9721 tgcattaacg caccaaaagg atcatcaccg acccgaccta taaacccact tattccgcct
     9781 aatctggcga ttcccaccgc aacgttagct ggcgcgccgc caggacaagg cagtaggcgc
     9841 ccgtctgatt ctggcaagag atctactacc gcatccccta aaacccatac tttggctgac
     9901 atttttttcc cttaaattca tctgacttac gcatagtgat aaacctcttt ttcgcaaaat
     9961 cgtcatagat ttactaaaac atgcatattc gatcacaaaa tgtcatagtt aacgttaaca
    10021 tttgtgatat tcatcgcatt tatgaaagta agggacttta tttttataaa agttaacgtt
    10081 aacaattcac caaatttgct taaccaggat gattaaaatg acgcaatctc gattgcatgc
    10141 agcgcaaaac gcactagcaa aacttcatga gcaccggggt aacactttct atccccattt
    10201 tcacctcgcg cctcctgccg ggtggatgaa cgatccaaac ggcctgatct ggtttaacga
    10261 tcgttatcac gcgttttatc aacatcaccc aatgagcgaa cactgggggc caatgcactg
    10321 gggacatgcc accagtgacg atatgatcca ctggcagcat gagcctattg cgctagcgcc
    10381 aggagacgat aatgacaaag acgggtgttt ttcaggtagt gctgtcgatg acaatggtgt
    10441 cctctcactt atctacaccg gacacgtctg gctcgatggt gcaggtaatg acgatgcaat
    10501 tcgcgaagta caatgtctgg ctaccagtcg ggatggtatt catttcgaga aacagggtgt
    10561 gatcctcact ccaccagaag gaatcatgca cttccgcgat cctaaagtgt ggcgtgaagc
    10621 cgacacatgg tggatggtag tcggggcgaa agacccaggc aacacggggc agatcctgct
    10681 ttatcgcggc agttcattac gtgaatggac tttcgatcgc gtactggccc acgctgatgc
    10741 gggtgaaagc tatatgtggg aatgtccgga ctttttcagc cttggcgatc agcattatct
    10801 gatgttttcc ccgcagggaa tgaatgccga gggatacagt taccgaaatc gctttcaaag
    10861 tggcgtaata cccggaatgt ggtcgccagg acgacttttt gcacaatccg ggcattttac
    10921 tgaacttgat aacgggcatg acttttatgc accacaaagc tttgtagcga aggatggtcg
    10981 gcgtattgtt atcggctgga tggatatgtg ggaatcgcca atgccctcaa aacgtgaagg
    11041 ctgggcaggc tgcatgacgc tggcgcgcga gctatcagag agcaatggca aactcctaca
    11101 acgcccggta cacgaagctg agtcgttacg ccagcagcat caatctatct ctccccgcac
    11161 aatcagcaat aaatatgttt tgcaggaaaa cgcgcaagca gttgagattc agttgcagtg
    11221 ggcgctgaag aacagtgatg ccgaacatta cggattacag ctcggcactg gaatgcggct
    11281 gtatattgat aaccaatctg agcgacttgt tttgtggcgg tattacccac acgagaattt
    11341 agacggctac cgtagtattc ccctcccgca gcgtgacacg ctcgccctaa ggatatttat
    11401 cgatacatca tccgtggaag tatttattaa cgacggggaa gcggtgatga gtagtcgaat
    11461 ctatccgcag ccagaagaac gggaactgtc gctttatgcc tcccacggag tggctgtggt
    11521 gcaacatgga gcactctggc aactgggtta acataatatc aggtggaaca acggatcaac
    11581 agcgggcaag ggatccgcgt cacacttccc ccttcacgac cttcaataat atgcaatgca
    11641 gcttcccgcc cgataatgtc atgtggaagc tgaattgtgg tcagcggcgg taaaaacaga
    11701 tgcccgacgc caaccagatt atcaaagccc attacggcga catcctgcgg gatacgtacc
    11761 cccttagcca ggagaacctg ataagccaca aaggctgcgc gatcgttacc acatatcaga
    11821 acatcaaaat ctggtttgcc cgatttgaag tggtcattga gtaaacttgc gagatcggtg
    11881 tagtgatcat cacctgttgc catgtgaaat tgtttcacct cagccagatc tcgtccagca
    11941 tcacgccagg cctgctcaaa tccctgccga cgataccctg ttgccaacgc actttccggt
    12001 agccagaagc ataacggttg acgatagccc gccgcgagca aatgctgtgt tgattcatat
    12061 tgtgcagtgt aatcatcagg gatataactg ggtaacgctg ggtcatccgc cacacagttc
    12121 gccaatacaa tattttcacc atacagagac tcaggcagcg tgatatgtcg cagccccatt
    12181 gtagtataga taatgccatc cggacggtgg gcaagcagct gacgtgccgc gcgggcagcg
    12241 tcatcttcag aaaaaatatt gattaaaaaa ctattccagc cgaactcgct ggcggtttgc
    12301 tcaatggcaa gcagaatatc aacagagaaa ggagtggtag cagtgtcctg cgccagcacg
    12361 gcgagagtcg acggcttacg tccttgagcg cgcatcttac gggcggaaag atcaggaaca
    12421 taattcaggg tctggattgc ctgcaatacg cggtcacgcg ttgcaggacg cacagattct
    12481 gcattatgca tcacccggga gactgtcatc atcgacactc ccgccaggcg tgcgacatcc
    12541 tttaatgaag ccatacccaa gccgtttgcc gtaaaacggg cactgtagca gaaacagacg
    12601 tcactggcga gatccaacgc cctatcacct gacacggcaa tacaataaaa aataacaata
    12661 attcccggat aattgtaccc aattccgcct ctgttctcgc caacgagtaa ccatataggc
    12721 aagtaagtaa aacatgccaa ctggcatcgc caacctggag aagaaaatat aaatatggat
    12781 cattgtgctg ccgctgtatc ccgacatcag cccggaaatt attgcgattg ctatcggttc
    12841 aggtgcgatt ggctgcacga tcgttacgga ctcgcttttc tggctggtga agcaatattg
    12901 cggcgctacg ctcaatgaaa catttaaata ctatacgaca gcgacattta tcgcttcagt
    12961 cgtcgctctg gcgggcacat tcctgctgtc atttatcatc taagcgcaaa gagacgtact
    13021 atggaaaacg ctaaaatgaa ctcgctcatc gcccagtatc cgttggtaaa ggttctggtt
    13081 gctcttaaag aaaccacctg gtttaatcct ggcacgacct cattggctga aggtttacct
    13141 tatgttggcc tgaccgaaca ggatgttcag gacgcccatg cgcgcttatc ccgttttgca
    13201 ccctatctgg caaaagcatt tcctgaaact gctgccactg gggggattat tgaatcagaa
    13261 ctgattgcca ttccggctat gcaaaaacgg ctggaaaagg aatatcaaca accgatcagc
    13321 gggcaactgc tactgaaaaa agatagccat ttgcccattt ccggctccat aaaagcacgc
    13381 ggcgggattt atgaagtcct ggcacacgca gaaaaactgg ctctggaagc ggggttgctg
    13441 acgcttgatg atgactacag caaactgctt tctccggagt ttaaacagtt ctttagccaa
    13501 tacagcattg ctgtgggctc aaccggaaat ctggggttat caatcggcat tatgagcgcc
    13561 cgcattggct ttaaggtgac agtgcatatg tctgctgatg cccgggcatg gaaaaaagcg
    13621 aaactgcgca gccatggcgt tacggtcgtg gaatatgagc aagattatgg tgttgccgtc
    13681 gaggaaggac gtaaagcagc gcagtctgac ccgaactgtt tctttattga tgacgaaaat
    13741 tcccgcacgt tgttccttgg gtattccgtc gctggccagc gtcttaaagc gcaatttgcc
    13801 cagcaaggcc gtatcgtcga tgctgataac cctctgtttg tctatctgcc gtgtggtgtt
    13861 ggcggtggtc ctggtggcgt cgcattcggg cttaaactgg cgtttggcga tcatgttcac
    13921 tgcttttttg ccgaaccaac gcactcccct tgtatgttgt taggcgtcca tacaggatta
    13981 cacgatcaga tttctgttca ggatattggt atcgacaacc ttaccgcagc ggatggcctt
    14041 gcagttggtc gcgcatcagg ctttgtcggg cgggcaatgg agcgtctgct ggatggcttc
    14101 tataccctta gcgatcaaac catgtatgac atgcttggct ggctggcgca ggaagaaggt
    14161 attcgtcttg aaccttcggc actggcgggt atgaccggac ctcagcgcgt gtgtgcatca
    14221 gtaagttacc aacagatgca cggtttcagc gcagaacaac tgcgtaatac cactcatctg
    14281 gtgtgggcga cgggaggtgg aatggtgccg gaagaagaga tgaatcaata tctggcaaaa
    14341 ggccgttaat aacgtttcaa cgcagcatcg ccatcctttc cctgggtgag cgatgctgcc
    14401 gatggcgcag acttaagatc cccggtctta cccgctataa ccccctgctt ttaccatcac
    14461 ccaacgcctt tcgctgtaaa cggcggtttc gcaaaccaaa ccaaaaccgt aagtaagata
    14521 aaagcaatag ccgccatacg gaaaatctca tttgcagaaa tagaaagtga ctgctgggtt
    14581 atttcattat taatttcatt aagaactcct gaaagcgaac catagtattt atccataatt
    14641 tgtgacgatg aattaaacac ggggttaaat tgatcgatgg ttgctgtcaa ctgactatgg
    14701 tgtaacgatt cgcgtcgtcc ccacagcgtc attgtcaacg acgtaccaac ggatcctgac
    14761 aaggtacgaa aaaaattact catactcgag gcattggcaa atttattatc tggcaagcct
    14821 gaaaacgaaa tcgttgttaa gggtaaaaag aaacaggcaa cggcgaatcc ctgaaaaaac
    14881 tgcggcaaaa tgatacctgt aaaatcaatc gttggcataa atgtcacaga acgccagtaa
    14941 tagcaaaccg catacatcaa aaaactaaat gtcaccaaca cccgcatgtc aattttgttg
    15001 ccataacgtc ctatcaaagg cgaaatcaat agtggcatga tgccgatggg cgcataagca
    15061 agtccggccc atatcgcatt ataccccatc gtttcctgga gtaactgcgg cataaggact
    15121 atcgctccag agtaaaataa atacgcgcag gtgatactca caataccaat ggtgaaatta
    15181 cgggacttaa acaaactgag gtcaagaatc gggttctctg aggtcgactc ccaaatgact
    15241 aaagagatca gagaaataac tgatactact gttaatatta ttattgtact cgagttgaac
    15301 caatccagat cgcgcccttt atcaagcata atttgcaagc caccaacacc gagcactaac
    15361 agggtcagtc ctggtagatt cattttgacc ggtgaagtct cagtttctct tcctttaagt
    15421 aaggttaagc ataatgtcag gacgataatc cccataggga cattgattaa aaatatccaa
    15481 ccccagctaa agttatcgca aatatatccg cctaatatcg gcccacatat cggagcgata
    15541 atcacggtca ttgaccataa tgccagagca aatgttcttt tttctggtgg ataattcctt
    15601 aataataaac tctgtgacag tggaattaac ggccctgcca ttaacccctg aacgactcta
    15661 aaaaatatca gcacatcaag attggtcgat aaactacaca ttaatgaaga cagcgaaaaa
    15721 aaagtgactg aaagtaaaaa taaccttaat tcgcctattc tttgtgccag cctgccggta
    15781 acagggatcg caatggcatt tgctacgcca aacgaggtga taacccaggt gccttcgtct
    15841 gttgatgctc ctagaaagcc agatattgtc ggtattgcga cgttagaaat agtggaatcc
    15901 aacatttgca taaacgtcgc taatgacaat gcaatagtga cgcaccataa cgtcccaccg
    15961 gttaatggtg ccggagttga tttagtgatt gccattaaag ttgtccatta tgcgaaataa
    16021 tgttgctaat ttctttttct atcggactgg tatcgataac taaagcctta ctggtataag
    16081 ccggcatgga cgtcacggtt gaagccagct caggcatctc agcaatgtct tcgttcttcg
    16141 tatcaatagt tgcagtcatc gataaaccaa tgcgcaaggg gtgttccatg agttcttttg
    16201 gatcaagaga aacttcaacc ggtacacgct gaacgatttt gatccagttc cctgtcgcat
    16261 tttgtgcagg taataaggag aacgcattgc cggttcccat attgatccct gtcacccggc
    16321 catgaaacac aacattctca ccataaagat cgctgataat attgaccgat tgaccaatcc
    16381 gtacatccgt gagttgtgtt tctttaaagt tggcattaac ccacatttga cgtgctggta
    16441 cgacagccat taacgattgt ccgggactca ctgtttcgcc gacctgaaca cttcgctggg
    16501 caatatagcc ggtaaccgga ctcttaatat ccgtacgttt aagcgccaac caggcttctt
    16561 tggttgcatc cgcagcttca acgacttgtg gctgacggtt taatggtgtg ttcattacta
    16621 aagctttatt cgctttataa gcctggatag cggcattcaa tgccgctttg ctacttatta
    16681 acgtatcttt ggtatgctcc agcgtttctt ttgaaataac cccctgcttc gctaaaggca
    16741 cacgacggtt ataatcttct aaagattgtt gatactgaat acgtgctgaa gcgacttcgg
    16801 cactgtattg tttatcctgt aagtacagtt tattcgtttg ccgaacaata tttgccagat
    16861 tatttttagc tttattgagt gcgatagtgg catcagtttt atccagtgaa actaaaatgt
    16921 caccctgtcg aacgtagttc gtatctttat gattaactac agtgacacta cctgagactt
    16981 gtgcagaaat tggatctgca ttccccgtga cataggcgtc atctgtacta atcatgtctt
    17041 ctaattccat tgaccaatag gcataagcac ctgaaaacgc aataaataaa actatcgcca
    17101 ataaagaaaa gtattttctt ctgttagaat gttttttatt tgaattaatc tgttccactt
    17161 ttatctctca tttcgcatag atgaaattta tgaattgaat gaaaagagta ttcattcttc
    17221 atatcaaaca cagtgcatgt gttgcacatt atcccaggaa cgttggaggc ctaatttaaa
    17281 tgaggtgcaa aaaaaacaac agaggactaa accgtggctt ttgcaataca attcttacgc
    17341 ctgtaggatt agtgagaaga cttatagtgc taatgtgaaa ctataaatca tcggtacaat
    17401 ccctgatttt attgttgaca tttcatatat gccgactatt tatatggtat acttgtcgaa
    17461 ttatcttaaa ggaagctcaa attttcttat ttttattgag aaaatgagat gatgccttat
    17521 gtctgtatta ctacagggag aagggagatg cttcattgca aagggaataa tctatgaacg
    17581 caataattat tgatgaccat cctcttgcta tcgcagcaat tcgtaattta ttgatcaaaa
    17641 acgatattga aatcttagca gagttgactg aaggcggaag tgccgttcag cgggtggaaa
    17701 cacttaagcc tgatatcgtc atcattgatg tcgatatccc cggagttaac ggtatccagg
    17761 tgttagaaac gctgaggaag cgccaatata gcggaattat tattatcgtc tccgctaaaa
    17821 atgaccattt ttacgggaaa cattgtgctg atgctggcgc taatggtttc gtgagtaaaa
    17881 aagaaggaat gaacaatatc attgcggcta ttgaagctgc aaaaaatggc tactgctatt
    17941 tccccttctc tctcaaccgg tttgttggaa gtttaacgtc cgaccagcaa aaactcgact
    18001 ccttatcgaa acaagaaatt agtgtcatgc gctatattct tgatggcaag gataataatg
    18061 acattgctga aaaaatgttc atcagcaaca aaactgtcag cacttataaa agtcgcctga
    18121 tggaaaaatt agaatgtaaa tcactgatgg atctttacac attcgcacaa cgtaacaaaa
    18181 tcggctaacc acatgaagtt tttaccctat atttttcttc tctgttgtgg tctttggtcg
    18241 accataagtt tcgcagacga agattacatc gaatatcgtg gcatcagtag taacaaccgt
    18301 gtcacacttg atccactacg tctgagcaac aaggaattac gttggttagc gagcaaaaaa
    18361 aatcttgtga ttgcagtaca caagtcccaa acggctacgt tgttgcatac cgattcgcag
    18421 caacgggttc gtggtattaa tgctgattat ttaaatcttt taaaaagagc gttaaatatc
    18481 aaattaacac tccgggaata cgccgatcat caaaaagcaa tggacgcgct ggaagaaggt
    18541 gaagtcgata tagtgttatc acatttagtt gcttcgccgc ctcttaatga tgacattgct
    18601 gcaaccaaac cactgataat tacctttccg gcgctggtca ccacccttca cgattcaatg
    18661 cgaccgctta cctcatcaaa accagtaaat attgctcgag tagcaaatta tccccctgac
    18721 gaggtaattc atcaatcatt tccaaaagca acaattatct cttttacaaa tttatatcag
    18781 gcattagcat ccgtctcagc cgaacagaat gattacttta ttggtagtaa catcattacc
    18841 agcagtatga tttcccgcta tttcactcac tccttaaatg tagtgaaata ttataactca
    18901 ccgcgtcaat ataatttttt cttgaccaga aaagaatctg tcgttcttaa tgaagtactc
    18961 aatcgatttg ttgatgcttt gacaaatgaa gttcgctatg aagtatcaca aaattggctt
    19021 gatacaggaa acctggcctt tctgaacaaa ccattagaac tcactgaaca tgaaaaacaa
    19081 tggattaagc agcatcccga tttaaaggtg ctggaaaatc cttactcgcc cccctattct
    19141 atgacggatg aaaatggctc ggttcggggc gttatggggg acattcttaa tattattacc
    19201 ttgcaaacag gtttaaattt ttctccgatc accgtttcac acaatatcca tgctggaaca
    19261 cagctgaatc ccggcggatg ggatatatta cccggtgcta tttatagtga agatagagaa
    19321 aataatgttt tatttgctga agccttcata acaacgcctt acgtttttgt catgcaaaaa
    19381 gcgcctgaca gtgaacaaac attaaaaaaa ggaatgaaag ttgccattcc atattattat
    19441 gagcttcatt cgcaattaaa agagatgtat ccggaggtag agtggataaa agtcgataac
    19501 gccagcgctg catttcacaa ggtcaaggaa ggcgaacttg atgctctggt cgcgacacag
    19561 ttaaattcac gttacatgat cgaccattac tatcctaatg aactttatca ttttcttatt
    19621 cccggcgttc agaatgcatc actttcgttc gcttttcctc gcggagaacc ggaacttaag
    19681 gatattatta ataaagcact gaatgcaatc cccccaagcg aagttctgcg cctgacggaa
    19741 aaatggatta aaatgcccaa tgtgaccatt gacacatggg acctctatag cgagcaattt
    19801 tatattgtta cgacattatc cgttttatta gttggcagta gccttttatg gggattctac
    19861 ctgttacgct cagttcgtcg tcgtaaagtc atccagggtg atttagaaaa ccaaatatca
    19921 ttccggaaag cactctcgga ttccttaccg aatccaactt atgttgtaaa ctggcaaggt
    19981 aatgtcatta gtcataatag tgcttttgaa cattatttca ctgcggattt ctataaaaat
    20041 gcaatgttac cattagaaaa cagtgactca ccctttaaag atgttttttc taatgcgcat
    20101 gaagtcacag cagaaacgaa agaaaatcga acaatataca cacaggtatt tgaaattgat
    20161 aatggcatcg agaaaagatg cattaatcac tggcatacat tatgcaatct gccagccagc
    20221 gatcatgctg tttatatttg tggttggcaa gatattaccg agacgcgtga tttaattcat
    20281 gcactcgaag tagagagaaa taaagcgatc aatgcaactg tcgcaaaaag tcagtttctg
    20341 gcaacaatga gtcacgaaat aagaacacca ataagctcca ttatgggctt cctggaactt
    20401 ctgtcgggtt ctggtcttag caaggagcaa cgggtggagg cgatttcact agcctacgcc
    20461 accggacaat cactcctcgg cttaattggt gaaatccttg atgtcgacaa aattgaatcg
    20521 ggtaactatc aacttcaacc acaatgggtc gatatcccta ctttagtcca gaacacttgt
    20581 cactctttcg gtgcgattgc tgcaagcaaa tcgatcgcat taagttgcag cagtacattt
    20641 cctgatcatt acctggttaa gatcgaccct caggcgttta agcaggtctt atcaaattta
    20701 ctgagtaatg ctctcaaatt taccaccgag ggggcagtaa aaattacgac ctccctggtt
    20761 cacattgatg acaaccacgc tgtaatcaaa atgacgatta tggattctgg aagtggatta
    20821 tcacaggaag aacaacaaca actgtttaaa cgttatagcc aaacaagtgc aggtcgtcag
    20881 caaacaggtt ctggtttagg cttaatgatc tgcaaagaat taattaaaaa catgcagggt
    20941 gatttgtcat tagaaagtca tccaggcata ggaacaacat ttacgatcac aatcccggta
    21001 gaaattaccc aacaagtggc ggctgtcgag gcaaaagcag aacaacctat cacactacct
    21061 gaaaagttga gcatattaat cgcggatgat catccgacca acaggctatt actcaaacgc
    21121 cagctaaatc tattaggata tgatgttgac gaagccactg atggtgtgca agcgctacac
    21181 aaagtcagta tgcaacatta tgatctgctt attactgacg ttaatatgcc gaatatggat
    21241 ggttttgagt tgactcgcaa actccgtgag caaaattcgt ccttacccat ctgggggctt
    21301 acagccaacg cacaggctaa cgaacgtgaa aaagggttaa attgcggcat gaacttatgt
    21361 ttgttcaaac cgttgaccct ggatgtactg aaaacacatt taagtcagtt acaccaggtt
    21421 gtgcatattg cacctcagta tcgccacctt gatatcgaag ccctgaaaaa taatacggcg
    21481 aacgatctac aactgatgca ggagattctc atgactttcc agcatgaaac gcataaagat
    21541 ctacccgctg cgtttcatgc actagaagct ggcgataaca gaactttcca tcaatgtatt
    21601 catcgcatcc acggtgcggc taacatactg aatttgcaaa agttgattaa tattagccat
    21661 cagttagaaa taacacctgt ttcagatgac agtaaacctg aaattcttca gttgctgaac
    21721 tctgtaaaag aacacattgc agagctggac caggagattg ctgttttctg tcaacaaaat
    21781 gactaaatag cggctcccac aatgttcaaa tgtgggagct atttaccagc acatcttaca
    21841 tttatgatga gaactcctgg cgaatttgtt ccccatgctg gtcgagcgtt gccgctcccg
    21901 gcataacatg cgggtccgcg cagccactga tttttatcgg attacccggc atcattattc
    21961 ccccggcttc aatcaacata tttctcgcct gagtttgtgg caaattaatg gcctcagcca
    22021 cacttaataa cggcgcgacg ggcacaccaa cttcatgtat tctggctaac caaacttcag
    22081 ctgcctgtgt ttttaacgtc cgctcaatat attgtttaag aatagcctgg ttttgtacgc
    22141 gtaaaatatt gctgctaaat ctgggatcat taaccagttc cgtaagctcc agtgcctggc
    22201 ataacgcaga gaaaagcttg tcattaccac aacaaatcgt aatcggctta tcctgagtat
    22261 cgaaaacatc aaaaggtgcc atgtaggggt ggcgatttcc caaacgttgt ggtgacttcc
    22321 cagtcgcgat atatgccatc agcccgtgct ccagaaaact cagcgtggca tcaaacatcg
    22381 ctatatcgac atgcgcccct ctctggctct tttcgcggcc ataaagtgca ctcactattc
    22441 cactgaataa atagacaccg ccacatagat ccgcaagaga agtgccaacg cgcactggcg
    22501 gagcatcagg gtatcccgtt tccatcatta tcccgctcat tgcctgaatg atggtatcgt
    22561 aggcaggagc atcttttagc ggaccggtat gtccgaaacc tgacgatgaa gcatatatga
    22621 ggcgcgggtt gatttcttgt aacctttccc atgaaaaccc cagtttttcc attgtacctg
    22681 ggcgaaaatt ctcagctaat acatcagctt gtttaagcat atttataaat atacttttat
    22741 cgtgatcatt ctttaaatca agaaccacac tctctttacc atgattaata aaactgtaat
    22801 agagtgactg tccatccaca tagggaccaa atgtgcgggt atcatcacca tgacctggtg
    22861 gctcaacttt aattaccctt gcgcccatat tacaaagaag ttgagttccg aaagggccat
    22921 taaggacatg tgtcatatcg ataactaata agccttcaaa cggccctttg ctttcattat
    22981 ttgtcatttg cttatgcctt gaaaaaagcc gaaggtgaaa agacacttcg gcatttatgt
    23041 aaaatcaata acatgattaa ttatgaaacc agtcgactga cataaatcca caagggagcc
    23101 gtgacgacaa aacccagtac gctcacagcc aatgacgctg taccagtgcg ggtgtagaca
    23161 ttaaaccggc tggcaataat gattccggag aatgccggcg gtaatgcgcc tgccagtacc
    23221 atcatctgca gatgttcgct gttcaaatga catgccatac caacaagaag cagcgccagt
    23281 ggcatcagaa tcagcttcag gaaggtgtta taagcaattt cggcactgaa ctcgaattta
    23341 tgtgcagcca gagtcaaccc cgcagcgaat accgctaccc ctgagttagc cttcgcaatc
    23401 agattaaagg ttgggtccca tgctgccgga atttttaccc caaccagcac caggatcgtt
    23461 gccagaacag gtgcccatac tacaggctcc tttgccgcag aaattaatgc gctcagatta
    23521 ctattcttct taccatccgc tcctgaggaa ggattcagca aatacagacc aataggaata
    23581 gtaattgcgt taacaataat agaaataatt gctaccacta aacctgttga tacggaatca
    23641 ccataaatag gatcgagaac tgcaaatccc aggaatccaa tggtaggtga acctgcaatt
    23701 aatgcacata cagctgcttc tgcatgggta cgtttaaaaa atttgtagca accaaaccag
    23761 gagaaaaaga aacatccgac aataaccaca agtgatacaa gggtcagacg agtgtccgca
    23821 aaaatcattt ccctgtttgc ccgggtaata gaaacaaata gagccgcagg aagagcgtag
    23881 tttaatacca gtttattaaa tgcccgcgct tgatcttctg aaaaagtttc tcgtctgccg
    23941 ctaaaataac ccaacagcat gattacgata ataggcaata aatcgccaat aaaaaatgtt
    24001 agcataatag tatttccgat aagggtcccc tcaccttcgc ataatgagtt ggcaagagga
    24061 gacgatgtca gtatttaatt accagcgact tgttttgggt taagtttggt aatatggcca
    24121 ctttcagttc ctgctgcagg gtcgatgacc acattaataa tggtcggttt gcgcgactga
    24181 ataccggtgg ttaaagcatg acgaagttca tctgtcgtgg tgacgttata gccaacgcca
    24241 cgaaacgcat ccattaattt gtcatacctt gcatggtgca acagatctgt tggtgatggt
    24301 gcaccagcgc cactgagatc aacaccgtct cctctgtaga tgccgccatt attaaaaata
    24361 acgatcgtca ccggcaggtt atatcgacaa atcgtttcaa tctccatccc actgaaacca
    24421 aaagcactat caccttcaat ggcgacaacc ggagaaccag aggtcacgct agcaccgatg
    24481 gcatagccca taccgatgcc catgacaccc caggtaccac aatccagacg acgacgtggt
    24541 ttatacatat caataatatt tcgtgcatta tccagggtat ttgcaccttc attaactaaa
    24601 taaatatcct ggttctcacg caatacatcg cgcacagcac ttaatgcatt aaagtaattt
    24661 aatggttggg tatctgtact taatttttca tgcatttttt gtgcattttg ctgcttgtgg
    24721 atatttaaaa tatcgcgcca taccagtgga gtcgtaaatg tgttttgttt cagttctgcc
    24781 agcatacctt gcatactgga tgcaatatcg ccaacgactg gcacagcaat ggggcggttg
    24841 ctgtcaattt cctgcggttc aatatccagt tgaataaact gtgtatctgc cgcccatcct
    24901 tttttaccgt gtgccagtaa ccaattcagt cgtgcaccaa caagcatgac aacgtcagca
    24961 tttgccaggg caaacgaacg cgcagctgcc gcagaaagtg gatgcgtatc ttcgaggatc
    25021 cctttcgcca tagacattgg caggaatgga atctgggcac tttcaataaa ttcacgaagc
    25081 tgttcgtcag cttgtgaata cgccgcgcct ttgccaagga taattaatgg ccgttcagct
    25141 tttgctaaaa gcgaaattgc gctagtgact gacttcgggc atggcaataa tgctggcgac
    25201 ggattttcaa cttttacaat cgtggttaac gcttcgtctt tttccatcgt cgcggccagg
    25261 acatttgctg gcaaatcaag ataaactccg ccagggcgac ccgatactga gacccggata
    25321 gcgcgtgcca atgcaatgcc aagatcctgc ggctgattaa cgcgaaatgc tgctttggca
    25381 tacggttttg ccgcattcat ttggtccagc tcttcataat caccttgctg taggtcgacg
    25441 atcgcgcggt cgctggagcc gctaatcatg atcatcggaa aaccatttac cgttgcgttg
    25501 gccaatgcgg tcaaaccatt gaggaatcct ggcgcagaca ctgtcaggca gatccctggt
    25561 ttttgggtaa gaaaaccgct tgccgcagcg gcatagcctg ccgactgctc atgacgaaaa
    25621 ccaatataac gaatgccttc cgcctgggca tggcgcgcca tatccgtcac aggaatacct
    25681 acaacaccat aaatagtgtc aatattattc tgttttaatg cttcaacgat gatatgcata
    25741 ccatctgtca tttgaagttg atctgacatg attaataacc tcgaaatttt tataaattgg
    25801 gagccagaag cccccgttaa tatcagatgg cgtggttttg cttcattgca gcaatttcat
    25861 cgtcgctata acccagctcc tgcaatacag cagcggtatg ttcacctaat agcggcgcag
    25921 ctttaatatc cggcgtaaag tcagagaatt tcattggaca accaactgtc agatattttc
    25981 cacgcaacgg ttgttccact tcgacaacac tgccactttg gcgcaaagag ggatcaattg
    26041 aaatttcttt catacttaaa accggtgcac aaggaatatc aaattgagtc aaataggcca
    26101 ccgcttcatg tttatcaata gtgacagtgt atttttcaat ttcagcaaaa atatcgaaaa
    26161 tatgtggctg tcgggcatgg gctgtactgt atgccggatc ggtaatccaa tctggtttgc
    26221 cgatggcttt acaggtgttt tcccagtttt gctcctgaat agtgaaataa atataggcgt
    26281 taggatcggt ttcccaacct ttacatttca ggatccagcc aggctgaccg ccaccacccg
    26341 cattacctcc gcggggaact gcatcaccaa atgtgccatt cggatactgc gggtattctt
    26401 ccagataacc caatttatcg agacgctgct ggtcgcgtaa tttcacgcgg caaaggttca
    26461 atacggcatc ctgcattgac atggtgactc gttgcccacg ccccgttttt tcgcgatgca
    26521 gcaaagcagc aagtaaaccg atcagtaaat gcattccggt gttgctgtca cccaacgctg
    26581 cagcgcttac cagcggcgga ccgtcccaaa aacccgtagt ggatgccgcg ccacccgctg
    26641 cctgagcaac gttttcatag gcttttacat tcacataagg cgaacactca tcaaaccctt
    26701 tgatcgaacc aaaaatcaga cgtggattga tttcttgaat atgctcccag gtgaaaccca
    26761 tgtgatcaat ggcccctgga tgaaagttct cgactaagat atcagcttcg cggatcagct
    26821 tttccattac ctctttgcct tccgctgttt tggtatttaa ctcaatagaa cgtttgttac
    26881 tgttaagcat ggtgaagtaa agcgcatcga tatcaggaat atctcgcagc tggtgacgcg
    26941 ttacgtcacc aacgccggga cgttcaattt taataacgtc agcgccaaac caggccagca
    27001 tttgagtaca agatgggcca gattgcacac cggtgaaatc gagaacttta attccttgaa
    27061 gtggagttga catagcaata cctattagtt aattaaatat gtgttaattt agcgtttatt
    27121 taaggcataa cgcctctata aataatccgc aaaatatcgt cgtagcggca ctcgatattt
    27181 ttacattcct gtatcaagtg ctgaaaatca tttcatcgct acttccaatt cctttctgca
    27241 tggtaagtag tgaggacgaa aagaagaata tgataaatat tggttcgtgg taatcagaga
    27301 gagtcgcgac atttgtgatg ttattcttac aactgtaaga agcgccggtg atttacaagt
    27361 aagggtatga ttacgctttt taaagcgtac cataaaatat aacatattaa atgatttgtg
    27421 atgacacggg attattgcgg tttactttga ggaatataaa atgcgagttt atagaacagc
    27481 gagcgccaaa taattttatg accacaacct gtcgtattgt ggtcataaaa aattgtcggc
    27541 tggagggagt taaatccttt acctcaatat actgcctgtc agcattagtt cacaaacagt
    27601 gcttcgctat cgacgatgat tccatcttcc gcacctttaa gcgccaggtt tgcctcgtag
    27661 tattgctttt tatcaagcag tttctgcgca tcagcaagtg cattacgagt ctgtttaagc
    27721 ggcatcagat attggttttc cattacacca acaccggcca ggcgaagctc ttccattgcg
    27781 ccctttttat cccctttcgc cattttttca ttggcaattt ttatcgcggc ttctttttcg
    27841 ggtgtcgcta cataactctc agaaattccg acagatgcat taatgacaat atattggtca
    27901 tcattgacat tggtcttttt acctggctta gcgaactttg cccattcggt actgtcatct
    27961 gacaacaaag ctgaagcttc attggtcagt tcttttgcct tttccggatc gccatgaaac
    28021 agagccaggc gcgctacctg cacgtcacgc atcgcatata aaccttgttc agagatacgc
    28081 tctgctgctg ccgttttctg ggtttgctgt tgtgccgcta ccggtgcatt atcagcagcc
    28141 catacagttg aagatgccag aattgctgtg accatcgtag ccataattaa acgtttcata
    28201 cttacctcat tctttaacag ttttagaaaa cgccgccatc atattgggtg agcatgaaat
    28261 gagaaatcag agagcaacgg tcattttgaa agggttatct tacagttgta gttattatcg
    28321 caagaagtat tttagtctta caattgtagg aatatgcttc ctgtcacaga gaactattct
    28381 tatgtaccac aaagagatgt catttattat atcgatgtaa atattgatta attatatgta
    28441 aatacagcca taacatcgta cattctcagt gaaccatttt caggataact ttctatgaaa
    28501 gtaaacttaa tactttttag cttattttta ttggtctcta ttatggcatg caatgttttt
    28561 gcattttcta attctggtgg tggtagtgag acgagctata aagagccaga aaaaacgtca
    28621 gcgatgacga caactcactc tacaaaattt cagccatcac aggcgatttt gtttaagatg
    28681 agagaagatg caccaccatt aaacctcaca gaagaaacca cgcccccttt tccgacaaag
    28741 gcgagttatc ttattcatcc tgtgcgatag cgtctgtcac atggcaggtg agcaatcatg
    28801 tctgatgctg atgtgaacac ctgcccaaat gagccatcct gacccactac cgcctgccat
    28861 accccaccat aaagcgaatc atcaagccac aagccggggc aagaaacata aatatccccg
    28921 tcaccagttc attaccgcca gttaaccaca tggtgaagta ataaatgagt gcagcgagga
    28981 tcaccatgcc gaagaaagaa gagaccgccg ataccacgtt cagtacctga ccgatgtagc
    29041 cgcttacgca gacaatacac agggcgagaa acatccataa ataaatcata gctcaacctc
    29101 ctgccgcgct aaaaaacctg taacttcagt cactttaata gtaagacaat ccttaacctc
    29161 tggcaatttt tgctaagcat caatgaaaac agcatgttaa atgcaacact attgcatgcg
    29221 gaaaattttt tactttgcac gattaataat cagtagctga aagcagtcag ccagtgataa
    29281 agtagagcca ccgcattcat gctcctcgtg gttatgtcct gactagtctt tacactcttt
    29341 acaggaacca ttgtcgtaca tgatggccca accaattgag tgtaccgctg tcgacatgga
    29401 atcttctgcg atacaacaat tcgtatctac agaaggtaac tatgtttcca caatgcaaat
    29461 tttcccgcga gtttctacat cctcgctact ggctcacatg gtttggactt ggtgtactct
    29521 ggctttgggt acagcttcct tatcctgttc tctgctttct cggcacgcgt attggcgcaa
    29581 tggcgcgacc attcctgaaa cggcgtgaat ctatcgcccg taaaaacctg gaactttgct
    29641 tcccgcagca ttctgcggaa gaacgcgaga agatgattgc cgaaaacttt cgttcactcg
    29701 gcatggcgct ggtagaaacc ggcatggcgt ggttctggcc cgacagtcgc gtacgtaaat
    29761 ggtttgatgt tgaagggctg gataacctta aacgcgcaca aatgcaaaat cgcggcgtaa
    29821 tggttgtcgg cgtccatttt atgtcgctgg aactgggcgg ccgcgtgatg ggactttgtc
    29881 agccaatgat ggctacctat cgtccacata ataatcagct gatggaatgg gtgcagaccc
    29941 gtggtcgcat gcgctctaac aaagcgatga tcggcagaaa taatctgcgc ggcattgtca
    30001 gtgcactgaa gaaaggtgaa gcggtatggt ttgctcccga tcaggattat ggtcgtaaag
    30061 gcagctcctt cgcgccgttc tttgcggtag aaaatgtcgc cacaaccaat ggcacctatg
    30121 ttctctcccg tctctctggc gcagccatgt tgaccgtaac gatggtaaga aaagcggatt
    30181 acagcggata tcgtttgttc atcaccccag agatggaagg ctacccgaca gatgaaaatc
    30241 aagccgctgc ctatatgaac aagattatcg agaaagaaat catgcgcgca ccggagcagt
    30301 acctctggat ccaccgtcgc tttaaaacgc gcccggtggg agaatcgtcg ttatacattt
    30361 aacaaactgc tctgactctt tacctcctca cataagccat aataacctca tggtttttat
    30421 ggcttttttc ttaaggtttt ttccttcgct gaatatctaa ttattaccgt cagaaattaa
    30481 actgtcgccg atatgagaca cctgtaagtt actaaaagta aataagaaaa tgcctcttgt
    30541 ctcgtttcag ttttaggcgt acattagcgc cgtctcgagc agtttagctc agaattctgg
    30601 ggtgatgccc acggtaaaat acaggacttt taaggtcgtt ttgcccggtg tcaactcact
    30661 gtatcaggtg taatgaagtc attcaggcgt aacagcaatt acgcggagag atgtaaagtg
    30721 aaatatttct ttatgggcat ttcttttatg gtcatcgttt gggccggtac ttttgccctg
    30781 atgatctaaa agcaaactgt gcaatgaaaa acaggagcca tcaggctcct gtttgcattt
    30841 ctgaggtcgg tgtttattcc gcgttttcgt gaatatgttt gctgctggcg ggtagtaaac
    30901 cgtcggcacg gaacatcgct ttaatcccac gaatcgcctg acgaatacgg tcgcggtttt
    30961 caatcagtgc aaagcgcaca tgcgtatcac catagtcgcc aaagccaatc cccggagaga
    31021 cacagacctt cgcttcgtta agcaatttct tggcaaattc cagcgagccc atggcagcat
    31081 acggttccgg tattttcgcc cagacataca tcgaagcctt cggcatttcg accatccagc
    31141 ccgcttcatg cagcccttta accaatacat cacggcggcg tttgtactgt tcggcaatgt
    31201 cgcgcacgca ctgttgatcg ccctccagcg ccgcaatcgc cgccacctgc aacggcgtaa
    31261 aggtgccgta atcgtgatag cttttaatac gtgccagcgc actgaccagc gttttgttgc
    31321 caaccataaa gccgatacgc cagcccgcca tgttgtagct tttcgacagc gtaaagaact
    31381 cgaccgccac atcgcgtgca cctgacacct gcatgattga cggcgctttc cagccatcgt
    31441 agacgatatc ggcataggcc aggtcatgga ccaccagcac atcgtagcgc ttcgccagcg
    31501 ccacaacctt ttcgaagaac tccagttcca cgcattgcgc ggttgggttc gacgggaagc
    31561 cgaggatcat catcttcggt ttcggataac tttcacgaat ggcgcgctcc agttcgttga
    31621 agaaatcgac accttccacc agcggcactg agcgtacctg agccccggca atcaccgcgc
    31681 cgtaaatatg aatggggtaa cttggattcg gcaccagcac tgtgtcaccg tgatccagtg
    31741 tcgccagcat cagatgcgcc aggccctctt tcgaaccaat agtgacgatg gcttctgatt
    31801 ccgggtcgat ttcaacgtcg tagcgatcct gataccagcg ggaaatggcg cgacgtagcc
    31861 gcggaatacc gcgtgaagtg gagtaaccat gcgtgtccgg gcgctgggcc acagtacata
    31921 atttttcgac gatatgcggc ggagtcgcac cgtccgggtt acccatactg aaatcgataa
    31981 tatcttcgcc gcgccgacgc gcagccattt tcagttcagc ggtaatgtta aaaacatagg
    32041 gcgggagacg atcaatgcgc gtaaagcgac gttcagggcg agtgtcagcc ataatttcct
    32101 cagattaacg ttagcgcccg gaccgtccga gcgacgctgc cacgatggtg gctgttttga
    32161 aaatagcctg attaatttct gtctgtccag cgttttagaa catttttatt aatgacgtta
    32221 aatgaggaac tctgctttaa aaacaggaaa attatctgcg ctaacaagaa aattaccact
    32281 cattttatta acataataat ttcaataaat ttactaacca gaagtgcatt atcaagagat
    32341 gcattgctaa tacaccattt atctatcaat ccccttttaa aattgtggtt tttcctcaaa
    32401 aacggcctgc tggtcatcag ccttcaggtt acctatcata gaggtttaat ccttattcag
    32461 agtcacccgt gcacgaaata ttcaacatgc tgctggcggt cttcgatcgg gcagcgttaa
    32521 tgcttatctg cctgttcttt ctcatccgta tccgcctgtt tcgcgaactg ttgcacaagt
    32581 cggcacactc accgaaagag ttgctcgccg ttaccgccat tttctcgctg ttcgccctct
    32641 tcagcacctg gtccggcgtt cccgtagaag gctcgctggt gaacgtacgt attatcgcgg
    32701 tgatgtccgg cgggattctg tttggcccgt gggtgggcat cattaccggc gtgattgcgg
    32761 gtattcatcg ttatttaatt gatatcggtg gcgtgacggc gatcccctgc tttatcacca
    32821 gcattctggc tggttgtata tcgggctgga tcaacctgaa aatccccaaa gcacagcgct
    32881 ggcgcgtcgg tattctcggc ggcatgttgt gtgaaacgtt gaccatgatt ctggtgattg
    32941 tctgggcacc aactaccgcg ttggggatcg atatcgtctc taaaatcggg attccaatga
    33001 tcctcggcag cgtctgtatc ggctttattg tgcttctggt gcaaagcgtt gagggcgaaa
    33061 aagaggccag cgccgcgcgg caggccaagt tggcgctgga tatcgccaac aaaacgctac
    33121 ctctgtttcg ccatgtcaat agcgagtcat tacgcaaagt ttgtgaaatt attcgcgatg
    33181 acattcacgc cgatgcggtg gcgattacca ataccgatca tgtgctggcc tatgttggtg
    33241 tgggtgaaca taactatcag aatggcgatg acttcattag cccgactacc cgtcaggcga
    33301 tgaattacgg aaaaatcatc attaaaaaca atgatgaagc ccaccgcaca ccagagattc
    33361 attccatgct ggtgatccca ttgtgggaga aaggcgttgt gaccggaacg ctgaaaattt
    33421 actactgcca cgcgcatcag ataacctcgt cattacagga aatggcggtt ggtctgtcgc
    33481 aaatcatctc cacgcaactg gaggtttcac gcgccgagca gctacgtgaa atggcaaata
    33541 aggcagagct tcgcgccctg caaagcaaaa ttaatcccca ttttctgttt aacgctctga
    33601 acgctatttc atcgtcgatc cgtctgaatc cggataccgc tcgccagctg atctttaatc
    33661 tgtcgcgtta tctgcgctat aacattgaat taaaagacga tgagcaaatc gatatcaaaa
    33721 aagagctgta tcaaattaaa gactatattg ccattgagca ggcccgcttt ggcgacaagc
    33781 tcacggttat ctatgatatt gatgaagagg tgaattgctg cattcccagc ctgctgatcc
    33841 agccgttggt ggagaatgcc attgtccacg gtatccagcc ttgcaaaggt aaaggcgttg
    33901 tcaccatcag cgttgcagag tgcggcaatc gggtacgcat tgccgtgcgg gataccgggc
    33961 acggcatcga tccaaaggtg attgagcggg tcgaagcgaa tgaaatgccg ggcaataaaa
    34021 ttggcctgct gaatgtccat catcgcgtga agttattgta tggcgagggg ctgcatatcc
    34081 gccgcctgga accaggaacg gaaattgcgt tttacattcc taaccaacgc accccagtcg
    34141 cctcacaggc tacgttattg ctttgagccg gagtgatatt gtgaaagtca tcattgttga
    34201 agacgaattc ctggcacaac aggaactgag ctggctaatt aaagagcaca gccagatgga
    34261 gattgtcggc acctttgacg acggtctgga cgtgttgaag tttttgcagc ataaccgcgt
    34321 cgacgccatt tttctggata tcaatattcc gtcgctggat ggtgtgttgc tggcgcaaaa
    34381 catcagccag ttcgcccata aaccgtttat tgtgttcatc accgcgtgga aagaacatgc
    34441 ggtagaagcg tttgaactgg aggcgtttga ctacattctc aaaccgtatc aggagtcacg
    34501 cattaccggg atgctgcaaa aactggaagc ggcctggcaa caacagcaga ccagcagcac
    34561 gcctgccgcg acggtaacgc gtgagaatga caccattaat ctggtgaaag atgagcgaat
    34621 aatcgtcacg ccaattaacg atatctatta cgccgaagcg cacgagaaaa tgacctttgt
    34681 ctatacgcgg cgtgaatcct acgtaatgcc gatgaacatt accgaatttt gcagcaaact
    34741 gccgccgtcg cattttttcc gctgccatcg ctcattttgt gtcaatctga acaaaatccg
    34801 cgagatcgaa ccgtggttta ataacaccta tattctgcga ctgaaagatc tggattttga
    34861 agtgccagtc agccgcagca aagtaaaaga atttcgccag ttaatgcatc tttaatcggg
    34921 aactttcatg aaagcaccag gcttgcccgc cgatcaacaa tttttcgccg atctgttcag
    34981 cggcctggtg cttaacccgc aactactggg gcgcgtctgg tttgccagcc agcctgcctc
    35041 gttgccggtg ggcagtttat gtattgattt tccccgtctg gatatcgtgc tgcgcggcga
    35101 atacggcaat ctgctggaag caaagcagca acgtttggtg gaaggagaaa tgctgtttat
    35161 tccggcgcgc gcggctaatt taccggtcaa caacaaaccg gtgatgctgt taagcctggt
    35221 gttcgctccg acctggcttg ggttatcgtt ttacgatagc cgcaccacgt cgttgttgca
    35281 tcctgctcgc cagatccagc ttcccagcct gcaacgcggt gaaggtgaag cgatgcttac
    35341 cgccctcacc catcttagcc gttcgccgct ggagcaaaat atcattcagc cgctggtgtt
    35401 aagtttgctg catctttgcc gcaacgtggt gaatatgccg ccgggtaatt cgcaaccgcg
    35461 cggcgatttt ctctatcaca gcatttgtaa ctgggttcag gataattatg cccagccgct
    35521 cacccgtgag aacgtggcgc agttttttaa tatcacgccc aatcatctgt caaaactgtt
    35581 tgcgcagcat ggaacaatgc gttttatcga atatgtgcgt tgggtgcgaa tggcgaaggc
    35641 gaggatgatt ttgcagaaat atcatctgtc gattcatgaa gtggcgcagc gttgcggctt
    35701 tccggacagc gactattttt gtcgcgtctt ccggcgtcag tttggcctga cgccgggaga
    35761 gtacagcgcc cgttttcagg gctaacgtca gaaggttaat tctgtttcca gcagcgtcag
    35821 gatactttgt gcatcttgcg cggcaaacaa cgactggcgg aagtttttat tcaccagttt
    35881 acgcgccagc tgcgagaaga ctttcacatg attcatccct tcgttagcgc ccagcgtcag
    35941 catgattacc agttcgactt cgcccatctc tgactgccag tcaaccggct tcaccagccg
    36001 ggcaatgctg atactggaat gacggatcca ctgagatttg gtgtgcggga tcgccacgcc
    36061 aaaaccaacg ccggtggtga caatctcttc ccgctgccag acatcctctt ccagctcgaa
    36121 cggatgttca gtgcgcccga taacgccgag gttaccgcac aggaactgga tcgcctgctc
    36181 tttattgcta aaggcctgat caacgaagat attctccagc gccaacaatg ggcgaacgtc
    36241 ttcttccggc gtaaaggcgg tgagtaacgc ttcgatttcc tgcgcgctgc gacattcaca
    36301 cgcctgacgt gccagttccc gacacgcctc gctatccagt tgacgcagct ggcttttcac
    36361 cgccggaatg cgcgggctac tcatactcag ctcatccagg tctagcccaa gcagtagcgg
    36421 cagataacgg atttcaccgc ccagttcacc gcaaatgcct acccatttgc cccgctgatg
    36481 cgcagtggta acaatctgct gcaacatgcg caggaacgat ggcgtaatcg ggttataaag
    36541 cggcgatacg cgcgggttat tacgatcgac cgcatacaga tactgggtca tatcgttgga
    36601 gccgatactg aagaaatcga cctcatcgca gaagtgatcg atgatgtagc acaccgacgg
    36661 cacttccacc atgataccca gcgtaatcgt ctctgcatga cgcaggccat cgcgcttaag
    36721 ctcaacgatc gctttttgga tctcgccctt cacccataag atctgatcga ggctgtgaac
    36781 catcgggatc atcaactggg cgttgccgaa actggcggcg cgcaaaatgg cccgcagttg
    36841 agtgcggaac aggccagcaa attccgggta aatacgtacc gcgcgatagc cgaggaacgg
    36901 gttctcttcc tggggaatat tcagataagg aatgctttta tcgccgccga tgtccattgt
    36961 gcggaagata atcggcttgt cgcccgccgc cagtagcacc tgctggtagg cttcaaattg
    37021 ctcctgctcg tccggcgcgc tgtcgcgatc catatagagc atttcggtac ggaacagccc
    37081 gacgccttcc gcgccgttgg caaacgcgcc aggcgcttcc agagcggtgc caatattcgc
    37141 cgcgatatca atacgcttgt tatcacggga ataagccagc tgcgcggcag cctgcgcctg
    37201 ttgtttttgg cgtttatccg ccagcgtctg cgcgacctga taataaccgc tcaccgcgtc
    37261 attcgggtta atcgccagca cgccgcactg ggcgtcaagc actgcaggtt gcccggcata
    37321 acgggcaatc gcatccagcg gcaggccgct cagtaccggg atcgccgagg cgcgggccag
    37381 aatcagggta tgcgaggtgc gcccggtttt ctccagaatc atgcccgcaa gattttttaa
    37441 atccaggctt aaaaactggc ttggggttaa atcctcggcc accagaatag tcggtttttc
    37501 cagcaccaga ttgttgcgcg gcttcagttc cggccaggta atatgcagca actgttcgct
    37561 aatatcgcga atgtcgctaa cacgttcgcg cagataatcg ctggcagaag cagaaagttt
    37621 ggcgcaaacc tgctccatat tgctgatgat cgccgccccc agcccctgat gctgttctgt
    37681 catcaggcga cggatattgc ctgcaaattc atcatcctga atcagcgaca aatgggcgct
    37741 gaggatagtt ttgctttcgc cgtcacgctc acgcaattgc tggttcagtt gctcggcaag
    37801 cgttgccagg ctgtgctcca gccgggtgga atcttgcgca ctggcgggga ttgcccgata
    37861 actgtcgagg ctgtcgcttt gtaacagggt cagcgtaccc acgccgacgc cgcttgccag
    37921 cacattgccg tacagtaaat ccgggttaag gcggcttaat gaacgcggca gcggatgcgc
    37981 cgtcagttct gcctgcgtag gctgaacgct gtcgctgtcg ataaagcgca cctggatgta
    38041 ctcttccagc acgcgccgcg cctgctcttc atcgctgccg ctaatgttca ggctgcaact
    38101 gtcattaaat agggtgccgg tgccaatcag cgccagcgag cttttggcat cggcctttgc
    38161 gttctggcga tggttaataa aagtgatttc gctttgccac tggctgcact gttctttaag
    38221 ttcccacgcc ggacgagcat gtagaccgtt aggcaaagga cagagaaatt gaatcgttaa
    38281 cataaccgct ccgggttaat caggagatca tctgaaatcc gtcagttgaa caaccgtctc
    38341 acgcgtaaga cgttgaataa gggcagataa aagttgctcc atctgcaaaa tgtcgcggca
    38401 atcggcaatc gatgcggcgc aatgtccgtg gcgggtggca ggccccatca ccactgtggg
    38461 tacgccggta ccggttaagt gcaccgctcc accgtcagtg ccgccgttac tgaacatatc
    38521 cgcctgtaac ggcacgccaa tttctgccgc cacggtttcg atccaggcgg tgagttttgg
    38581 cggcgcaatc agtgacttgt cgcttaacac cagcatcggg ccgttaccaa tctggcgatg
    38641 gttagccgcg ccataatcaa agtttttcgc ccagcaggcg gtatcaagga caatggcgac
    38701 gtccggcgac accgcgcggg tggcagtttg cccgccgcgt aatcccacct cttcgctgga
    38761 acttgccacc agccacactt ccgcaggcag ttcggcgtcg tgcagctcac gtagtaacgt
    38821 caccagcaga tagcaaccga ggcggtcatc aaaggctttc cccatcaccc gctggtgggg
    38881 gagaacctga aaagtggtat cgaacgtgac gcgatcgcct ggacgaattc ccgcctgcat
    38941 cacttcgtca tacgagcgcg cgccaatatc cacgcgcatg gcgctgacat cattcccctg
    39001 ccggtcgcca tcaagcagac cgggaatttt gcactcttca cgggtggtga tgcgcaccgg
    39061 ctgcagctgg cgggcagcca tgcgtacgtt gccaaccggc agtacatcaa tcgccccttc
    39121 gcgggagatg ctgcgcacca taaatcccac ttcatccata tgcgcacaga tcatcacctt
    39181 cggacctgtc gattcattca ggcggatcag caccgatccc agaccatcaa atcgcacttc
    39241 tttttgcagg cgatccgctt cttccagcag gatctgccgt acttcctgtt ccgaggaggc
    39301 gatcgcatct gcctcgctca acgcttttaa tagcgataaa tccattatgc ctctcccgtg
    39361 agcaacactg ttttcggcat ggcgtagagc acttctgcgc cttgcggggt gaccagcaca
    39421 acatcctcga tgcgcacgcc cccttgccct ggcaaataaa tccccggctc cacggtcagt
    39481 aacatgcctg gctgtagcgt cgtggtatcc cgcggtgaaa agcgcggatc ttcgtgtact
    39541 tcaatgccaa tagcatgacc ggtgttgtga ccaaaatagt cgccaaatcc tgcctcggta
    39601 atcacccgac gcgcggcttc gtcaacctgc tggcagcgca caccggggcg aattgcagag
    39661 attgctgcga gctgtgcctg caaaacaatc tgatagacgt taaacagcgg atgagattcg
    39721 gtgctcaccc cttcgccatt caccagcaag gtgcgcgtca tatcagagca gtagccctga
    39781 tacagcgcac cgaaatcgag agtgacaaac tcgcccgctg caacaatctt gtcgctggct
    39841 ttgccgtgcg gcagcgcccc acgccagcca ctggcaacaa tggtgtcaaa agaggttttt
    39901 tctgcgccct gctggcgcat aaaccactcc agttcagcgg ctatctcgcg ctcgctcatc
    39961 cccgcctgaa taaagcggcg aatatgctct gcaccgcgat cggcaatccc acaggcaagg
    40021 cggattttct ccacctcctc tggcgttttg atttgccgca gcacatccgg cgtggcgcta
    40081 accagtttcg cattgagttc agactgccag cggtgcgcgg tttcccaact cacctgctgg
    40141 ccttcaaaac cgagcgtttg caactgttca tcggcaatga tttcattgac gatggttgca
    40201 agcgtgtgtg tcgcgtcaag caaatgcagc tggtaacctt gcgtacgagc ttctacatcc
    40261 gcgtaatagc gcgagtccac cagaatgtgc gcactttcac ggctaatcag cacatagcct
    40321 gatccggtgg agatccccag atgcggctgt ttgttctgcc gtgaggagag aagcactgca
    40381 tccagttgtt gcgccttaag ccagtcgcgc agcgaagcga gtaatgtcat ctggattttc
    40441 cttcttacag gctatcgatt aacaatttgc ctttacggaa catcatcaga cgcaggaaca
    40501 ccaccatcag cgcagtaatg actgccccca gcgcgatccc cgccatatag acgcccaggt
    40561 tagtgaccag cggccatgcc cagatagcgg attccgggaa ccattgcact gcgcccagcc
    40621 agacagcggc ggttgagccg acaatcgcgc cgaccatata cgacggaatg gcggtgatgg
    40681 ggctttccag cgcaaacgga atcgcaccct cactgatccc cataaaggca aggaacatcg
    40741 cggtcttacc ctgcggataa agttgtgcgt tgaacaggcg tttgccggtt aaacgacggt
    40801 caataatggt cgccagcccc agaccaatcg gcggaataac gatagcgata gaacgtgcgg
    40861 tgaccggtaa tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg gctttgttga
    40921 tcgggccacc aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc
    40981 ccttctcacc tgcggcagtc agcacagtac ggataccgcc gttgatccag ccaccaaagg
    41041 gggtgatgac gtagtacatc gccagcatga caaaaatagc ggaaagaatc ggtagcaaaa
    41101 atgtggtttt gaacgctaac aggaaatccg gtaactggat tttctggttc atccatttca
    41161 caaggtaacc agcaacgata gaaataatca gggcaccaat gaaggtggac ggcaccggcg
    41221 aagaggtcgc ccattgcata gtgctgggat cgaagttcag cagttgcgtc ggctgggtag
    41281 acatcaagcc gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca
    41341 caaatgcagc aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca
    41401 accaggcaaa tttcagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat
    41461 ccatgatgcc gatatcagca ggaattttaa gccagctata agcaatcagc tggctgaaag
    41521 cgaggatcac accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat
    41581 gctgcgggag ttcaccccag aagctgcttt tagaggcctg cggattctta actgccgctg
    41641 ccgcaccgga tgcgccaggc acaacggttg cactgcgttt tttaatggcc ataacaatta
    41701 tccctgtcga atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgatgc
    41761 ccgcagcgtt tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac
    41821 gttcgttatc ttccggggta actgccacgg aatggatgat gatggtcgct tcggcgatat
    41881 cctgcgccgt caggcgattc tggataccgt ccgcgccctg ggtttcaatt ttcacttcat
    41941 aaccggcttc taccgctgct tcttccagcg cctgagcggc cataaaggtg tgagccaggc
    42001 ccatcgggca ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg
    42061 atagttgacg agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag acaaataatg
    42121 catttactgg agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg
    42181 gacacaaaga aaaattgaat taactgtatg atttaaaaga ttatcgggag agttatctcc
    42241 cgatataaca ggaaggattt acagaatgtg ccctaaggtc tggcgtaaat gtgcaccgga
    42301 accgagaaga cccggattgt catggacgat gagatacacc ggaatatcat ggacatattc
    42361 tttaaagcgc cctttatctt caaatgcggc acggaaacca gaggctttga agaactcaag
    42421 gaagcgcggc acgataccgc ccgcaataaa cacgccgcca aatgtcccga gattgagcgc
    42481 cagattgccg ccaaaacggc ccataatgac gcaaaacagc gacaacgcac ggcggcaatc
    42541 ggtgcagctg tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag
    42601 gcggttgtcg gctttaacaa ttgcgcgata caaattcact agcccagggc cagaaagcac
    42661 gcgttccgcc gaaacatggc caatttccgc acgcagtatt tcgaggataa tggcctcttc
    42721 ttcactattc ggcgcaaaat caacgtgacc gccttcgccc ggcaggctta cccaacgctt
    42781 atcgacatgg accagatgcg caaccccaag ccccgttccg gcaccgtaaa ccgcaatagg
    42841 cttaccttct atcggctctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg
    42901 aatcgccatt gatacagcgg taaaatcgtt aataatttcc agatggctaa aaccgagatt
    42961 ctttttcatt tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc
    43021 ggtaattggg caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag
    43081 ataaacgcga atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg
    43141 cgagatttca ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc
    43201 accgactaat gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt
    43261 ctcacactgt aaatacctgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa
    43321 tatcgatctg ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt
    43381 tgcaagctgg gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc
    43441 gagccagaac catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc
    43501 ttattctgat tgtggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac
    43561 tgccgggaac tctggggata gcccaggatt cacccctctg gatcatcggt gtattaacgc
    43621 taacgggtat tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagacc
    43681 ccgcctgtga accgctgatc ggcgcaccgg ttccgccctc tgcgctacct ggacttatcg
    43741 tagcattaat tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcatga
    43801 ccgtcaatat cgcccttgcg gttgccattg gcgctcgtct gttaccgcgc gtcaaccgaa
    43861 tggagtggac tattttagcc tctgccggaa ccatcggcgc gctgtttggt acacctgttg
    43921 cggcggcgtt gatattttcg caaaccttaa atggcagtag tgaagttccg ctatgggatc
    43981 gtctttttgc gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc
    44041 atccccattt ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca
    44101 gcggtgcaat tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac
    44161 cacggttgca cgcgatgatg catcaaatga aaaatccggt gctcgtgctg ggtattggcg
    44221 gatttattct cggtattctg ggggttattg gtgggccggt ttcgctgttt aaaggactgg
    44281 atgagatgca gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg
    44341 cggtgattaa actgaccgcc ctggtcgttg ctgccgccag tggctttcgc ggtggacgaa
    44401 ttttcccggc agtgttcgtc ggcgtggcat tagggttgat gctgcatgag cacgtccccg
    44461 ccgttccagc ggcaataacc gtttcctgcg ctattctcgg catcgtgctg gtggtaacac
    44521 gcgatggctg gttaagcctt tttatggcgg cagtcgttgt acccaatacc acattgctac
    44581 cgctgctctg tatcgtcatg cttccggcat ggctgttatt agcaggtaag ccgatgatga
    44641 tggtcaatcg tccgaagcaa cagccccccc cacgataacg tttagcaaaa aatgctctcc
    44701 tttatgattt aagagttatg gcgatgatac ggggctttac ctccccgtaa tattgcgtta
    44761 acaggccgct gacggcttcg atcgtgaagg agaataacaa tgttcaggtc actgtttctg
    44821 gcggccgccc tgatggcatt taccccgctt gcagcaaacg caggcgaaat caccctactg
    44881 ccatcaatca aattacaaat tggcgatcgc gatcattacg gtaattactg ggacggtggt
    44941 cactggcgcg accgtgacta ctggcatcgc aattatgaat ggcgtaaaaa ccgctggtgg
    45001 cgtcatgata atggctacca tcgtggctgg gataagcgta aagcatatga gcgtggctat
    45061 cgtgaaggct ggcgcgatcg tgacgatcat cgcggaaaag gccgcggaca tggacaccgc
    45121 cattaaacac attcgccaat ggagcacaat gcctgatgcg acgctagcgc gtcttatcag
    45181 gcatacaaac cggctcttta acgcgtaggg cggataaggc gtttacgccg catccgccaa
    45241 tagtgccaga tgcgacgctc attcaactac aatcccagcg ccgtccccac caacaaccag
    45301 atattcagcg ccacgaccag cactacaatc acccagcctg tctgttttac acgtttgctg
    45361 ttcaccagat cgcccatcaa cttgctgtca ctggtgaaaa tcagcagtgg aaccagcgcc
    45421 agggcgatac caaaacttaa cagtacctga ctcataacca gaatccgtgt cggatctaat
    45481 cccatcagaa tgacaataaa tgacggcagc atggtgactg tacgacgcac ccacagcggg
    45541 atatgaaagc gaatgaagcc ctgcatcacc acctgccccg ccagtgtccc caccaccgtt
    45601 gaggacagtc cggcagcaac caggcttaat ccaaagaccg ttgccgcagc atggcttaac
    45661 agcggttgca gcgtcagata agcctcatca agatcggcaa caccagtatg accagaaaag
    45721 tggaacgccg ccgcagctgt agccatcatc gccagattga caaaaccggc aatcgtcatg
    45781 gcgatagcca catcccattt ggtggcggaa taacgttgtt gacgcgaacc gccatgtaaa
    45841 tgctgagtga gcgaagagtg caaataaatc acatgcggca taatcgtcgc ccctaacacg
    45901 cctgctgcca ggaagaccgc ctccgaagta ggtaaactcg ggatcaccat tcctttaccc
    45961 agctgcgcca ggttaggctg ggagaaaatc aactcgacaa tgtaagccgc ggcaacaaac
    46021 aacagtaacc cgccaatcac tttctccagc ggtttttgcc cgcgacgttg cagcattaaa
    46081 atcaggaaag tcgcgatccc cgtcagcacc gcgccctgca acaacgaaac accaagaatg
    46141 agtttaaaac cgatcgccgc accaataaat tccgccaggt cggttgccat cgcaataatt
    46201 tctgcctgaa cccaatagaa ccacactacg ggacgcggat agtgatcgcg aatctgctcc
    46261 gccagatttt taccggtggc aatccctagt ttggcagaga ggatctgaat cagcatcgcc
    46321 atcaggttgg cccaaacgac aacccacagt agctgatagc cgaagctggc acccgcctga
    46381 atattggtcg caaagttacc gggatcgata taaccaatcg ccgcaatgaa cgcaggtccc
    46441 attaatgcga gcctcatctt gcgcgccgcc cgtccgctgc tactctcaac gcgatagttc
    46501 gtcatcttgt gcctctaaaa catagccttt gctatgtttc atgctatgcc aaacgagaat
    46561 gattatcaaa ttcatttaaa tgggttgtgg tgatttctct gatagaccag gattatgact
    46621 acgaaaagat tgatgacttg aatgttatgg ttatgtttaa tgttagcaca tttacataac
    46681 tttcagcttc catacacaac atagcagaaa tgtatgacag ctcactattt ttgaagcttg
    46741 tcacaggacg tcattatagt gtgtgtcaga tctcgttttc cttaaccatg ttacatagaa
    46801 tgtgcacgga aatttaacct gcctcatatt tggagcaaat atggaccgcg tccttcattt
    46861 tgtactggca cttgccgttg ttgcgattct cgcactgctg gtaagcagcg accgcaaaaa
    46921 aattcgtatc cgttatgtta ttcaactgct tgttatcgaa gtgttactgg cgtggttctt
    46981 cctgaactcc gacgttggtt taggcttcgt gaaaggcttc tccgaaatgt tcgaaaaact
    47041 gctcggattt gccaacgaag ggactaactt cgtctttggt agcatgaatg atcaaggcct
    47101 ggcattcttc ttcctgaaag tgctgtgccc aatcgtcttt atctctgcac tgatcggtat
    47161 tctccagcac attcgcgtgt tgccggtgat tatccgcgca attggtttcc tgctctccaa
    47221 agtcaacggt atgggcaaac tggaatcctt taacgccgtc agctccctga ttctgggtca
    47281 gtctgaaaac tttattgcct ataaagatat ccttggcaaa atctcccgta accgtatgta
    47341 caccatggca gcaacggcga tgtccaccgt ttctatgtcc atcgttggtg catacatgac
    47401 catgctggag ccgaaatatg tcgttgcagc gctggtactg aatatgttca gcacctttat
    47461 cgtgctgtcg ctgatcaacc cttaccgtgt tgatgccagt gaagaaaaca ttcagatgtc
    47521 caacctgcac gaaggtcaga gcttcttcga aatgctgggt gaatacattc tggcaggttt
    47581 caaagttgcc attatcgttg ctgcgatgct gatcggcttt atcgccctga tcgccgcgct
    47641 gaacgcactg tttgccaccg tgactggctg gtttggctac agcatctcct tccagggcat
    47701 cctgggctac atcttctatc cgattgcatg ggtgatgggt gttccgtcca gcgaagcact
    47761 gcaagtgggc agtatcatgg cgaccaaact ggtttctaac gagttcgttg cgatgatgga
    47821 tctgcagaaa attgcttcca cgctctctcc gcgtgctgaa ggcatcatct ctgtgttcct
    47881 ggtttccttc gctaacttct cttcaatcgg aattattgca ggtgcagtta aaggcctgaa
    47941 tgaagagcaa ggtaatgtgg tttctcgctt cggtctgaaa ctggtttacg gctctaccct
    48001 ggtgagtgtg ctgtctgcgt caatcgcagc actggtgctg taagaccata cataaaaaag
    48061 ccggggataa ttccccggtt tttttacgcc tgttaatcag ctaatggctg cgggcgacca
    48121 atcaaatacc cctgcagata ttgcaccccg agcttatgca ataacgcctg ctgctgttgc
    48181 gtctcgacaa actccgcgac cacactcaat gacttcgctt tcgccagatc ggtaattgat
    48241 ctcacaatca tcgcatccag cgtattcgtg acaatatctt tcacaaagac gccatcaatt
    48301 ttgatgatat cagcctgcaa acgctttaac cgttcgtagt tggcatagcc ggtgccaaag
    48361 tcatcaatcg caatccggaa accaaactta tgcagctgct cgatgttgta catgctgctt
    48421 tctgcgttag aaaacgcctg ctcctcggtg atctcaagaa tgaccgcctg cggggagatg
    48481 tgataacgct taaatagacg aataatccgc ccggcaatat tcttttgcag cagcgtgagc
    48541 ggcattaaat taactgaaaa gcgcgggcct tttttgtcgc aagggtgtgt tgccagccac
    48601 ttcaacaagg attccagcac ttgcaaatca aaacgcgcgc taagattaaa ctgagcaata
    48661 aggggcagaa acttatccgg ggtcataatg ccgccgtcat atttcagtcg cgcgaggatc
    48721 tcatcataac cttcaccctc tttgttgcga attggctggg cgtagagcag caaatcaccc
    48781 tgatctaacg ccgtgcgaat ggtattcagc aatagcacct gtttagtggt ctgcccggaa
    48841 accatctcct ctcggctatc cagcgccagc acatgatgat gtgcgcagga ttgctccgcc
    48901 agccagctta actgccccaa caagggttgc agggtttcct gatttccatc aaaacgcccc
    48961 caggcagcac catagcccat atccagcccg gtattgttcc agtgaatttg ccgactattc
    49021 aggatgttaa ccatatgctg gagtcgccct tccgtttccg gcccgctcag cactaacagc
    49081 agttcactac ccggcaattg atacaacttt tcgttttcct gcatcaatgg cagcagcgta
    49141 cggtaaattg atcggataca gtgaacgcgc atcatcaacc cgtaatgacg actcataaac
    49201 tcaagattat caatgcgcag gcagcaaaaa ctcttgcccg cctcttgctc cggcgcttgt
    49261 tccaacgcac gaaagttggg caggagcgtt aacggatcgg tcagcgcctg caaatgccag
    49321 cggcgattaa gccattcact gcgatgataa atgcgcacca tatagagcag gcaaacgcta
    49381 aaggaaatca gcaccgcgag aataaatgcc agcgaatatt cggtttccac cccttgcaaa
    49441 aagttctggt tgtaattcag caggcaaagc gttgaaacag cccaggtgag atttaaaaac
    49501 ggatagcgaa gcttaccgac cccgagggta aagatgatga agaaaacggg taccaggtaa
    49561 cctgcaataa agtcgttttc ataaggtgtg cacagcagaa gtagcagcac gcttaacgct
    49621 gccagccagc ttaaggtaaa tgcgcgtttc tctttgccca atgacggagc gatatccctg
    49681 cgccacaata tctgcgcaaa gtggggactt acaatcatgc gagtgagata gtagaaaagc
    49741 atgttgtaaa tcagaacagc ggtgaaaagg cttagtaaat ccacgaccgt gaaaatggca
    49801 tccgcatcgc cgaaaaaggt ggatatcttt agcgggaaat caaagaagct ccccacaaga
    49861 tacatgctgc atttgatgcc gatcggcgtc accaaaccaa gccagaacag acgttgccag
    49921 acatatcggc tggtcaatcc ataacgccaa cgcgtcccca gctgccagcg taatattgcg
    49981 caggcacaca acacgacaaa tgtctggctg ccgagcatga ccgcagtctg taaaaaagag
    50041 agatgaaagt tccacagatt ggtgcaaaac atcccgcata aaacgggaac gacaccgcgc
    50101 cagccaaaga taaacaagat tgacagcatt acgcacagtg gcatccacgc cagaaagata
    50161 taactggaat ggataatggc aagtggcgaa ataaaacggg atagctgaat aagtaccacg
    50221 gtgagcgtaa acgctagtgt gaatatcttg atatttttta tcaggttatg ctccacaaac
    50281 atgagtacac tactctcact gatcggggtt ttcaggcaca caaataataa gtttattcgc
    50341 attgcgaagc aaacaggggc catcctgttg ccgatttttc atccaatgat gctttcacca
    50401 ggcggcacta acccggacgt agactgtatt acaaaagcgg caaaaagcag agacaaaaaa
    50461 cccccgcttt gcagcgaggg ttggaaattt ggtggagcta agcgggatcg aaccgctgac
    50521 ctcttgcatg ccatgcaagc gctctcccag ctgagctata gccccacgat gcgtttacgt
    50581 accaaatttg ctgggtgcaa aatttggtgg agctaagcgg gatcgaaccg ctgacctctt
    50641 gcatgccatg caagcgctct cccagctgag ctatagcccc gtcacgtaaa gcttgtcgag
    50701 ttgacgggcg gcatcatatg aattccgccc gaatgtgtca acggcaaatt gcagcgtgta
    50761 gtttcaatcg ctgaaaaatc aggcaaatga acaattttgg aaaaccgctc gcattcagta
    50821 gttattcatg tcacggtttc ctgtaaagtg gtgctataaa atgaactact aatagaccca
    50881 catacattca gggaattgtt atgttcaagg agaggatgac gccagatgaa cttgccagac
    50941 tgaccggcta tagccgccag accattaata aatgggtacg caaggaaggc tggacgacgt
    51001 caccaaaacc aggcgtccag ggtggcaaag cccgactggt tcacgtcaat gaacaggttc
    51061 gtgaatatat tcgtaatgct gaacgtccag aaggtcaggg agaagcgcct gccctttctg
    51121 gtgatgcgcc gcttgaagta ttgctggtga cactggcgaa agagatgacg ccagttgaac
    51181 aaaaacagtt tacatccttg cttctgcggg aagggattat cggattgtta caacgcttag
    51241 ggattcgcga tagcaaataa tatgaaaaga ttacgcaata aaatgaccac cgaagaactg
    51301 gctgaatgcc tcggtgtggc taaacaaact gttaaccgtt ggatcagaga aaaaggctgg
    51361 aaaacggaaa aatttcccgg cgtgaaaggc ggtcgtgcca gacttattct ggtcgatacg
    51421 caagtttgcg agtttattca gaacacgcct gcgttccata acacaccaat gttgatggaa
    51481 gctgaagaga gaattgctga atacgctcct ggagcacgcg ccccggcgta tcgacaaatc
    51541 atcaacgcga tagataatat gacagacatt gagcaggaaa aagtcgcgca atttttgtca
    51601 cgtgaaggaa ttcgcaattt cctcgcccgt ctcgatattg acgaatcagc ataaataaaa
    51661 aacggcagga tattatctcc tgccgtttat ctttttacac gctaattact gctggttttc
    51721 gcgttcagca ataaaggcca gcgctttgtt gatacgctca atgctacgtg tcttaccgat
    51781 tgcgtgaacg gtaacatcca gtgccggaga ctgccccgca ccggttacgg cgacacgcag
    51841 cggcatacca actttaccca tacccacttc cagctcatcc gccgtcgcct gaatagcgtg
    51901 atgaacgttt tcagctgtcc agtcagtaat cgcggccagt ttgtcacgaa ctacttccag
    51961 cggctgacgc gctaccggac gcagatgttt tttcgcggca tcggcatcga actcagcaaa
    52021 atcttcgtag aaataacggc agctctgtgc catctctttc agcgtcttgc agcgttcgcc
    52081 cagcagtttc accagatcag ccagctgcgg gccgttacgg gtatcgatat tttcctgctc
    52141 aatgtgccac tgtaagtgag tagcaacata ctccggcggc agcgcgttaa tgtagtgatg
    52201 gttcagccac agcagcttgt cggtgttgaa cgcactggca gatttgctga cggcattcaa
    52261 agtgaagtat ttgatcatct cttcacgagt gaagatttcc tgatcgccgt gggaccagcc
    52321 cagacgcacc agatagttca gcagtgcttc tggcaaataa ccgtcatcac gatactgcat
    52381 tacgctgact gccccgtgac gtttggacag ttttttgccg tcatcgccgt tgatcataga
    52441 aacgtgcgcg taaaccggta ccggcgcttt cagggcttta agaatgttga tctggcgtgg
    52501 cgtgttgttg atatggtctt cgccacggat aacgtgggtg atttccatat cccagtcatc
    52561 gacaaccaca cagaagttat aggttgggga accatcggta cggcggataa taagatcgtc
    52621 cagttcctgg ttgctgaact cgatcggacc acggatctga tcgtcaaaaa caacagaacc
    52681 ttcctgcggg ttagcaaaac gtacgacaca cggctcatca tcagcgtgat gctcatgact
    52741 gtggcggcag cgaccgtcat aacgcggctt ctcacctttc gccatttgct cttcgcgcag
    52801 cgcttccagg cgctctttag agcaatagca tttataagct gtgccctctt ccagcatctg
    52861 atcgatcacc gcgttgtagc gatcaaaacg tttggtctgg tagtacggac cttcatccca
    52921 ctccaggctc agccagttca tgccatccat aatggcttcg atagcttccg gcgtggaacg
    52981 ctcaagatcg gtgtcttcaa tacgcagcac gaactcaccg ccgtggttac gtgcaaaaag
    53041 ccaggagtaa agagcagtac gcgcgccgcc aacgtgcaga tagcctgttg ggcttggcgc
    53101 gaagcgagtt ttgattttca tgaaatggcc ttacgtttag aaagatgccg acaaccggca
    53161 aatcctggaa aaattgagtg ggcgatattc tatcactccc gcctgattcc tcaatgtagt
    53221 tcgggttgca accttacgca ttattgtttt cgttgacaaa ttgcgcaaca cggtctgttt
    53281 tgcgatcatc ttgcttaaat ttacgacgaa cgaataattt ctttagaaaa tgcgttgact
    53341 cattttgaac tctccctata atgcgactcc acacagcggg ggtgattagc tcagctggga
    53401 gagcacctcc cttacaagga gggggtcggc ggttcgatcc cgtcatcacc caccaactac
    53461 tatatgtagt ctccgccgtg tagcaagaaa ttgagaagtg ggtgattagc tcagctggga
    53521 gagcacctcc cttacaagga gggggtcggc ggttcgatcc cgtcatcacc caccacttcg
    53581 ggtcgttagc tcagttggta gagcagttga cttttaatca attggtcgca ggttcgaatc
    53641 ctgcacgacc caccaatgta aaaaagcgcc ctaaaggcgc ttttttgcta tctgcgatac
    53701 tcaaagattc gaacctgcag caggttcgag ttgagcgcag cgaaacaacg gagccgctcg
    53761 cggcgacggc ccgaagggcg agcgaagcga gtcatcctgc acgacccacc aatgtaaaaa
    53821 agcgccctaa aggcgctttt ttgctatctg cgatttgtaa gattgatttt tcttctcaag
    53881 ttccttgttc ttctaccgat attacctcgt acagtgacga ggactgaaca atggccacta
    53941 tctcttcaac ttcaattcct tccatacaaa cacagagtag taacagggca tcacaaggta
    54001 gcgatgttgc ttcacaaatc gcgcggataa gtcagcaaat catcaaactc actcaacaaa
    54061 ttaaagagat tgtagacact agcggcagtg ctgaagacaa acaaaaacag gccgaattga
    54121 ttcaacagca aatcacccta ctggaaacac aactggcgca gttgcagaga cagcaggcag
    54181 aaaaggcgca ggaaaaagag cagcgtttgt ctctgaatgt atcccttcta aatcctgttg
    54241 aaaaaacgac tcatatcgat atctatatct gatacaacgg atccccttcc cgcccagcca
    54301 ttagcgcatt gccctggtct cgtaccacgt cccataacgc ttcagcggcg gtggaaagtg
    54361 aacggttctt cctgcgcacc agcatcaact ggcgttcaac aaccggcgta atgcgcttta
    54421 ccaccagcgg gctaccttca ggcaatggta gcgccagtgc gggaagaata ctgatgccga
    54481 tgcctgcggc taccatcggg aatagcgtcg ccggatgtcc aatctcctgc acaatattcg
    54541 cctgaatacc attgcgcgcc agcgctgcgt caatcagcgg tcggctgcct gacgcgtaat
    54601 cctgcaacac caatttcgct ccctgtaatg cctgccaggg tacataatct tccgcggcca
    54661 aagcgctatc ccgatggcaa agcagaaaga aaggctcgga aagaatcgct tcacattgca
    54721 gatcgcccac tgggcctgga tcgatgacga tgccaaagtc gacatctccc tggcgaatac
    54781 tttccatcac ccactgctgc ggacgatcgt gcaggacaaa ctgaatatct ggatagcggc
    54841 gatggctttc agcaatgcat tgcggaataa gatgcgcgga aatggtctgg ctggcagcga
    54901 cccgaacttt tccgctcagt tgttgcccca tacgcccggt atcgcgcaac gtgctgttaa
    54961 gttcatccag cagtcgctca agacgcaaag ccaactgctg ccctgcatct gtaagcacca
    55021 cttcacgcgt ggttctgtcc agcaggcgaa caccagtatg attttccagc tccttcacac
    55081 tgtgacttac tgccgactgg ctcaggccga tacgctctcc tgcacgacta aaacttttct
    55141 cctgcgctac tgtgacgaaa acctttaatt gttttaaaga ataattcatc tgttttcttc
    55201 atgaatggat gcaataaatc aattttattt ctcaaactgg aagaagcaca atagaaccat
    55261 cgatcatctg gagtctttat gaaacttttt cgtatcctcg atcctttcac cttaaccctg
    55321 atcacggtgg tgttgctggc ctctttcttt ccggccagag gcgatttcgt ccccttcttt
    55381 gaaaatctga ccaccgcagc tattgccctg ctgttcttta tgcacggcgc gaagttgtcg
    55441 cgtgaggcga ttattgctgg cggtggtcac tggcgactgc atttgtgggt aatgtgcagc
    55501 accttcgtgc tgtttccgat tctgggtgta ctgtttgcct ggtggaaacc ggtaaatgtc
    55561 gacccgatgc tctactccgg ttttctctac ttgtgcattc tcccggctac cgtgcagtct
    55621 gcaatcgcct tcacgtcaat ggcgggcggt aacgtcgcgg cggcggtttg ttctgcgtca
    55681 gcatccagcc tgctggggat tttcctttca ccattgctgg ttggtctggt gatgaatgtt
    55741 cacggtgcag ggggcagcct tgagcaggtc ggtaaaatta tgctgcaact gctgctgccg
    55801 tttgtgttgg ggcatctttc ccggccgtgg attggtgact gggtgtcgcg caataaaaaa
    55861 tggattgcga aaactgacca gacgtccatt ctgttggtgg tttatacagc gttcagcgaa
    55921 gccgtcgtta atggtatctg gcataaagtt ggctggggat cattgctgtt tatcgtggtg
    55981 gtcagctgcg ttcttctggc tatcgtgatt gtagttaacg tctttatggc acgccgactg
    56041 ggcttcaata aggcagatga aattactatc gtcttttgtg gttcgaaaaa gagtctggca
    56101 aatggcatcc cgatggcaaa cattctgttc cccacatcgg tgatcggtat gatggtgctg
    56161 cccctgatga ttttccatca gatccaattg atggtctgtg cggtgctggc gcgtcgatac
    56221 aaacgccaga ccgaacagtt acaggcgcag caggaaagca gcaccgataa agcttaaagc
    56281 ggacgcttca ggggctggac cagttgcgtc agcccctcgg ttttaatcag cagcgtgatt
    56341 tgcattagct caccgagctt ccctgccggg aactcatctt tgcgggcaaa ccacagcaga
    56401 tactcctccg gcaggtcgat taagcgacgc cctttgtatt tgccaaacgg cattatcgta
    56461 ttggctattt caatcagctg ctctttttcc atctcagcta cccagcaaac gcagcatttc
    56521 cgcttcgtcg atgacttcaa tgcccagttc ctgcgccttc gccagtttag atcctgcagc
    56581 ttcacccgct atcaccagat cggttttctt cgacacgctg cccgcgactt tcgcgcccag
    56641 ttcgaccagt cgagctttag cgtcatcacg cgacatctgg cttaagctgc ccgtaagcac
    56701 cacggtttta ccagcaaacg ggctgtcaat ctcttccgcg ttgataacga tcggcgcagg
    56761 ccagtgaaca ccttccgcca acagctcgct gatgacattg cggttgcttt cttcggcaaa
    56821 gaagttgtga acgtgggatg caacgacaat gccaacatca ggcacctttt gcagctcttc
    56881 aatcgaagcg gcttccagcg cttccagcgt gccgaaatat gccgccagac ctgctgcggt
    56941 ggcctcgccg acttcacgga tgccaagtgc atagaggaag cgagcaaagg tggtttcttt
    57001 cgctttttcc agcgcgttaa ccacgttttg tgccgatttt ggccccatac gctccagtcc
    57061 ggtaagtttg cctgcggtga gtttgaacag atccgccgga gtgtggacat attctttttc
    57121 aaccagctga tcgatgattt tgtcgcccat tccgtcaaca tccatcgcac ggcgggaaac
    57181 aaagtgtttc agcgactctt tacgctgcgc accgcaaatc aggccaccgg tacagcgggc
    57241 aaccgcttca ccttccacac gctcaacgtc agaaccacat accggacaat gcgtcgggaa
    57301 tacaacctca cgagtatctt ccgggcgttc agaaagcacg acgttcacca cctgcgggat
    57361 cacgtcgcca gcgcggcgaa tcaccacttt atcgccaatg cgtaaaccaa gacgttcgat
    57421 ttcatccgca ttgtgtaagg ttgcgttact caccagcacg cctgcaacat ggacaggttc
    57481 cagacgcgca acaggcgtaa tcgcgccagt acgcccaacc tgaaactcga cgtcacgcac
    57541 aaaggtcatc tgttcctgcg ccgggaattt aaacgctacc gcccagcgcg gggctcgcgc
    57601 gacaaagcca agctgctcct gctgtgccag tgagttgacc ttaatcacca cgccgtcgat
    57661 atcaaagccc agcgtcgggc ggtcttcttc cactttgtga tagaacgcca gcacttcttc
    57721 cgccgattca caaagcgtta cccgatcgct gaccggcaac ccccactttt taaattgcag
    57781 taaacggcca agatgagtat ccggcagctc gccaccttcc agaacaccaa cgccatagca
    57841 gaaaaaagtg agcggtcgct tcgctgtaat acgcggatca agctgacgca gtgaaccagc
    57901 tgccgcatta cgtgggttag caaaaacttt cccgcccgtg cgtcgcgcat cttcgttaat
    57961 cttttcgaac cccgcctgcg gcaggaacac ttcaccacgc acttccagac gcgccgggat
    58021 attctctccg tgcagcttca gtggaatggc gcgaatggta cgcacgttag acgtgatatc
    58081 ttccccggtg gtgccatcgc cacgggtcgc ggcactgact aaaacgccat tttcatacag
    58141 aatactgaca gcaaggccat ccagcttcag ctcacagcac caggtgactt tctcgttgct
    58201 tttcagacgg tcctgcactc gtttgttgaa ggcaagaaag ctttcttcat caaaaacgtt
    58261 atccagcgac agcattggca cttcatggcg aatctggctg aaagccgcca gcggcgcagc
    58321 acctacacgt tgggtaggcg aatcaggcgt aatcagttct ggatgtttgg tttccagctc
    58381 gcgcagttcg cgcatcagcc tgtcgtattc agcgtcggga atttccggcg catccatcac
    58441 atgataaaga tattcatgat ggcgaagcgt cgttcgcagt tctgtcagtt gttgttcgat
    58501 tgattccata tcgcaccatc aatgctaaaa acccccgaca agcgggggtt cgaagaggag
    58561 ttaatttgcc ttaagtgtat caggcgttgg cgtctttaac ttcgcggatg atgtcctggt
    58621 actcacgcaa tttctgcgga gtcatcatac ggcgctgatc gtcaagcacg acaccgccca
    58681 cttcatcggc aatatgctgc gcagattgca gcatcagctt gaagttctgc agctcgtcac
    58741 cgtaagacgg tacctgcata aagatagtga cacccggagt agtgaaatcc ttcatttcag
    58801 gatcaaaggt tcccggtttc accatattcg ccaggctaaa taacgccggg ccgctgccat
    58861 ccgggctaag atgacgatgg taaatattca tatcgccaaa aatgaagccc gcttgttgaa
    58921 tgctgttaag aagcagttca ccgtttagct cgctaccgtg atgcgccgcg acgttcatga
    58981 taatcaccgc ttctttgcgc ttcggtttat ccataactgg agccggttcc gctacaggct
    59041 caggctgtgg tgccgctacg ggttctgcag gctggaaagc ctgttgtgcc ggttgcggtg
    59101 ctgaatgcac tggctgcggc gctggcgcga cctgtggcga aactggctgc tgcaacggct
    59161 gttcaggctg cggctgatag gcaggctgct gcacgggttg ctgcaccggc tgcgctggac
    59221 gcggagcatg ttgcggcggt acctgcgctt caggcggctg ctggaccggt tgacgcggct
    59281 gcgcagacgc ataaggcggt tggtactggt gttgcggcga cggacgagca gcctcatgct
    59341 cctgggcgtt agccggggca tgattcacgc ggtgaacacg aacctcacca acgccctcat
    59401 catcttcgac atcctcgtca taagaatcgt cgtcacgttt tgacttcatt cgttttaatg
    59461 gccgatcgcg gaacatagaa gatcgttctt tacggctggt ccagaaacca tgtaccagta
    59521 aagcgattat ggcgatcgcg ccaacaatga ttaatatcag acgcaaatcc tgcatcatta
    59581 tattctctgt tgttctaaca ccttgccacc acggcaaaca tttactcact aagagtattt
    59641 gccgattacc tcaagtgcaa gtgcactatt aactttcaca gcacaaagat agatgaaatc
    59701 gtgctttttg ctgttttttc gaacatatcc taactgtcca ttgcgcaatt acccggtaaa
    59761 atacgcacaa ttttcctggg attggtcaaa aggagctcat cctgattatg gtttcatcat
    59821 tcacatctgc cccacgcagc ggtttttact attttgcgca aggctggaag ctcgtctcgc
    59881 aacctgggat tcggcgtttc gttattttac cgctgctggt caatattttg ttgatggggg
    59941 gcgcattctg gtggctcttt acgcaactcg atgtctggat cccgactctc atgagttatg
    60001 ttccggactg gttacaatgg ctgagctatc tgttgtggcc tctggctgtc atctctgtgc
    60061 tgttagtgtt tggctatttc ttctccacga ttgctaactg gattgccgct ccgtttaacg
    60121 gtttattggc tgaacaactg gaagcacgat tgactggcgc tacaccgcca gataccggga
    60181 ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg cgaatggcaa aaatttgcct
    60241 ggtatctgcc gcgcgcaatt gtattactaa ttctttactt catccctggt attgggcaaa
    60301 ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat gttagccatc cagtattgcg
    60361 attacccctt cgataaccac aaagtgccgt ttaaagagat gcgcaccgcc ctgcgcacac
    60421 gcaaaatcac caatatgcag tttggtgctt taaccagcct gtttacgatg atcccgctgc
    60481 ttaatctgtt catcatgccc gttgccgttt gtggcgcgac ggcgatgtgg gtcgattgct
    60541 atcgcgataa acacgcgatg tggcggtaac aatctaccgg ttattttgta aaccgtttgt
    60601 gtgaaacagg ggtggcttat gccgcccctt attccatctt gcatgtcatt atttcccttc
    60661 tgcatataga tatgctaaat ccttacttcc gcatattctc tgagcgggta tgctacctgt
    60721 tgtatcccaa tttcatacag ttaaggacag gccatgagta agatttttga agataactcg
    60781 ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca tcggtaacgg acgcattctg
    60841 gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt gccgtatcgg tgccaacatg
    60901 atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg ttgaactggt tgaaccaacc
    60961 agcggtaata ccgggattgc actggcctat gtagctgccg ctcgcggtta caaactcacc
    61021 ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc tgctgaaagc gttaggtgca
    61081 aacctggtgc tgaccgaagg tgctaaaggc atgaaaggcg caatccaaaa agcagaagaa
    61141 attgtcgcca gcaatccaga gaaatacctg ctgctgcaac aattcagcaa tccggcaaac
    61201 cctgaaattc acgaaaaaac caccggtccg gagatctggg aagataccga cggtcaggtt
    61261 gatgtattta ttgctggcgt tgggactggc ggtacgctga ctggcgtcag ccgctacatt
    61321 aaaggcacca aaggtaagac cgatcttatc tctgtcgccg ttgagccaac cgattctcca
    61381 gttatcgccc aggcgctggc aggtgaagag attaaacctg gcccgcataa aattcagggt
    61441 attggcgctg gttttatccc ggctaacctc gatctcaagc tggtcgataa agtcattggc
    61501 atcaccaatg aagaagcgat ttctaccgcc cgtcgtctga tggaagaaga aggtattctt
    61561 gcaggtatct cttctggagc agctgttgcc gcggcgttga aactacaaga agatgaaagc
    61621 tttaccaaca agaatattgt ggttattcta ccgtcatcgg gtgagcgtta tttaagcacc
    61681 gcattgtttg ccgatctctt cactgagaaa gaattgcaac agtaatgcca gcttgttaaa
    61741 aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc ctgcttcaaa ctttcgcccc
    61801 tcctggcatt gattcagcct gtcggaactg gtatttaacc agactaatta ttttgatgcg
    61861 cgaaattaat cgttacagga aaagccaaag ctgaatcgat tttatgattt ggttcaattc
    61921 ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc agcggtcaac acccgccagc
    61981 aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa aaactggcgc taacaataca
    62041 ggctaaagtc gaaccgccag gctagacttt agttccacaa cactaaacct ataagttggg
    62101 gaaatacaat gttccagcaa gaagttacca ttaccgctcc gaacggtctg cacacccgcc
    62161 ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc tgaaattact gtgacttcca
    62221 acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca gactctgggc ctgactcaag
    62281 gtaccgttgt gactatctcc gcagaaggcg aagacgagca gaaagcggtt gaacatctgg
    62341 ttaaactgat ggcggaactc gagtaatttc ccgggttctt ttaaaaatca gtcacaagta
    62401 aggtagggtt atgatttcag gcattttagc atccccgggt atcgctttcg gtaaagctct
    62461 gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa atttctgccg accaggttga
    62521 tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca tcagcccagc tggaaacgat
    62581 caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa gccatctttg aagggcatat
    62641 tatgctgctc gaagatgagg agctggagca ggaaatcata gccctgatta aagataagca
    62701 catgacagct gacgcagctg ctcatgaagt tatcgaaggt caggcttctg ccctggaaga
    62761 gctggatgat gaatacctga aagaacgtgc ggctgacgta cgtgatatcg gtaagcgcct
    62821 gctgcgcaac atcctgggcc tgaagattat cgacctgagc gccattcagg atgaagtcat
    62881 tctggttgcc gctgacctga cgccgtccga aaccgcacag ctgaacctga agaaggtgct
    62941 gggtttcatc accgacgcgg gtggccgtac ttcccacacc tctatcatgg cgcgttctct
    63001 ggaactgcct gctatcgtgg gtaccggtag cgtcacctct caggtgaaaa atgacgacta
    63061 tctgattctg gatgccgtaa ataatcaggt ttacgtcaat ccaaccaacg aagttattga
    63121 taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa gcagagcttg ctaaactgaa
    63181 agatctgcca gctattacgc tggacggtca ccaggtagaa gtatgcgcta acattggtac
    63241 ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa ggcgttggtc tgtatcgtac
    63301 tgagttcctg ttcatggacc gcgacgcgct gcccactgaa gaagaacagt ttgctgctta
    63361 caaagcagtg gctgaagcgt gtggctcgca ggcggttatc gttcgtacca tggacatcgg
    63421 cggcgacaaa gagctgccat acatgaactt cccgaaagaa gagaacccgt tcctcggctg
    63481 gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg cgcgatcagc tccgcgctat
    63541 cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc ccgatgatca tctctgttga
    63601 agaagtgcgt gcactgcgca aagagatcga aatctacaaa caggaactgc gcgacgaagg
    63661 taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg gaaacaccgg ctgccgcaac
    63721 aattgcacgt catttagcca aagaagttga tttctttagt atcggcacca atgatttaac
    63781 gcagtacact ctggcagttg accgtggtaa tgatatgatt tcacaccttt accagccaat
    63841 gtcaccgtcc gtgctgaact tgatcaagca agttattgat gcttctcatg ctgaaggcaa
    63901 atggactggc atgtgtggtg agcttgctgg cgatgaacgt gctacacttc tgttgctggg
    63961 gatgggtctg gacgaattct ctatgagcgc catttctatc ccgcgcatta agaagattat
    64021 ccgtaacacg aacttcgaag atgcgaaggt gttagcagag caggctcttg ctcaaccgac
    64081 aacggacgag ttaatgacgc tggttaacaa gttcattgaa gaaaaaacaa tctgctaatc
    64141 cacgagatgc ggcccaattt actgcttagg agaagatcat gggtttgttc gataaactga
    64201 aatctctggt ttccgacgac aagaaggata ccggaactat tgagatcatt gctccgctct
    64261 ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt ttttgcggaa aaaatcgttg
    64321 gtgatggtat tgctatcaaa ccaacgggta acaaaatggt cgcgcctgta gacggcacca
    64381 ttggtaaaat ctttgaaacc aaccacgcat tctctatcga atctgatagc ggcgttgaac
    64441 tgttcgtcca cttcggtatc gacaccgttg aactgaaagg cgaaggcttc aagcgtattg
    64501 ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat tgaatttgat ctgccgctgc
    64561 tggaagagaa agccaagtct accctgactc cggttgttat ctccaacatg gacgaaatca
    64621 aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga aaccccggtt atccgcatca
    64681 agaagtaatt cttgccgcag tgaaaaatgg cgccgattgg cgccattttt ttatgcttcc
    64741 accagcggtg gcaaaatcaa ttcatcgctc tcatgttgct gggtgtagcg catcacttcc
    64801 agtacacgca aacccgcgcg gtgcaccgcg tcggttaacg ccttcccttt cagcaagcca
    64861 ctgatgagct gagcacaaaa caggtcgcca gtccctttca ggtcggtttt tacccgtgaa
    64921 tgggaaatga cattcacgct gtcggcagtg accaccacaa cctgcatctc ctgattttct
    64981 tcattaccgg aggcgctggt aatcaccacc cattttagtg tgtctgaaag cagacttttt
    65041 gcggcagcaa tggcactgtc gagatcgcgg caatttttac cggtcaggat ttccagctca
    65101 aagatattgg gggtaattcc ctgcgccagc ggcagtaaat attgtcgata cgcctcggga
    65161 aggtcaggtt tgacataaat tccgctatca atatcgccaa tcaccggatc gaccatgatc
    65221 aataggtcag gatggtcttt gcgtagcgca gtcagccact cggcaaggat tttgatttgc
    65281 gatgccgttc ccatatagcc cgtggttaca gctcgaagtt ggcgcagcgc atcacgctcc
    65341 tgaagcgcac gcaaatagcc gctaaaccat tcgtccggaa tcgcaccacc gtagaaagtg
    65401 tcataatgcg gcgtattgct cagcaatacc gtcggcacgg caaagacatt caggccgttc
    65461 tgtttgatag caggcacggc aatgctgttg cctacgctgc cgtacactac ctgcgactgc
    65521 acggcgacga tatccgcctg cagtgccctg ctcttatcgt taaacaacaa caaactactc
    65581 atttaatttt ttctccttgc cgatgatcct catcgtattc caaccgaaac tttacctgat
    65641 tctggcagtc aaatcggcta ttacaaaaca aggataaggt aattcagtga agaaaatcat
    65701 ttgtctggtc attacactac taatgacact ccccgcttac gcgaagttaa ctgcccatga
    65761 agaagcccgc atcaacgcca tgctggaggg attagcacag aaaaaggatt tgatatttgt
    65821 gcgcaacggt gatgaacata cctgcgatga agcggtttct catctgcgtc tgaagctggg
    65881 caatacccgt aaccgcattg acactgccga gcagtttatt gataaggttg cttcgtcgtc
    65941 atcgattacc gggaagccgt atattgtgaa gatccccggt aagagcgatg agaacgcaca
    66001 gcctttttta catgcgttaa ttgcgcagac ggataaaaca gtgcctgcgc aataatctta
    66061 aatccccgcc ctctgactaa aatgctcttc cccaaacacc ccggtggaaa ggtagcgatc
    66121 gccacgatcg cagatgatcg ctaccaccac cgcaccaggg ttagcttttg ccacccgcag
    66181 tgctccggca accgcgccgc cggagctgac gccacagaat attccttccc gcaccgccag
    66241 ttcgcgcatg gtgttttccg catcgcgctg atgaatatcc agcacctcat ccaccagaga
    66301 agcgttgaaa atccccggca gatattccgt aggccagcgg cgaatgccgg gaatgctgct
    66361 gccctcttcc ggttgcaggc cgacaatggt caccggtttg gattgttcgc gcataaagcg
    66421 tgacacgccg gtgatagtgc cggttgttcc catgctggag acaaaatggg taatgcgccc
    66481 gccagtctgc tgccagattt ccggcccggt agtggtgtaa tgggcgtaag ggttatcggg
    66541 attattgaac tgatcgagca gctttccttc accacgatcc gccatctcca gcgccagatc
    66601 gcgcgcacct tccatgccct gctctttggt gaccagaatc agttccgcac cataagcacg
    66661 catcgccgca cggcgttcct ggctcatgtt gtcgggcatc agcaatttca tgcgatagcc
    66721 tttaagcgcg gcaatcattg ccagcgcaat gccggtgtta ccactggtgg cttcgattaa
    66781 cacatcaccc ggtttaattt ccccgcgctt ttccgcctcg acgatcatcg aaagcgccgc
    66841 acgatctttt accgaacctg ccgggttatt gccttccagt tttaaccaca cttcactgcc
    66901 gttattcggc cccattcgct gcaacttcac cagaggcgta ttgcctattg tttgttctaa
    66961 tgtactcacg atctctatcc atacgtggtg ttgcctgatg cgacgcttgc gcgtcttatc
    67021 aggcctacac cattaaaaag cccggatcgc gggaagcgcc tccgggcgtt taacattcac
    67081 tcaacctatc aggcgctttg tgcgagagca agttcctcat ctcgggtttc gatacgctcg
    67141 tcgccgttat acagccgcgc atgttgcaga ccaacgaata aacgctcgcc acgctgcggg
    67201 gcattgtcgc catgcatcac gaccgtcagc ggttcgttgt accaccccag cggctgcacc
    67261 actaattggg tgtagtgacc tttcgggctg gcttccagta cctgtaccgg cagcggcgaa
    67321 tcgaggctgg tacggcggct gatatccact tcccaagggc gcaggaagag atccaccggc
    67381 ccctgatacg caggtgtgta gcccagcggc cagcgatgcg cgccaacatg gaactgcccg
    67441 ccgcgaatgg ttccctgcag gcggttcact tcgcccataa attcgagcac aaaacgggtc
    67501 gccggttcgc gccatacctg atccggcgcg tcagcctgtt caatattgcc ctggctcatc
    67561 acaactacac gatcagctac ttcggtcgct tcttcctgat cgtgggtcac aaaaacgctg
    67621 gtgaatttta gttcttcatg gagttgacgc agccagcgac gcagctcttt acgcacctgc
    67681 gcatccagcg cgccaaacgg ttcatcaagc agcagaattt gcggttccac agccagcgca
    67741 cgcgccagcg ccacacgctg tttctggccg ccggaaagct gcgccggata acgatccgcc
    67801 agatgagcaa gctgcaccat ctccagcaat tttgtcactt tcgctttgat cgccgcggca
    67861 ttcgggcgct cgcgacgcgg cagcaccgtc aggccaaaag cgatattgtc gaacaccgtc
    67921 atatggcgga acagcgcgta atgctggaac acgaaaccga ctttacgatc acgtgcgtgc
    67981 aggcggctca cgtcggtgcc gtggaagcga atatgcccgc tggtttgatg ctccagccca
    68041 gcgataatgc gcagcagcgt ggttttcccg gaaccggacg gccccagcaa cgcgaccatc
    68101 tgacctgaag gaatatccag tgagatatcg ttcagcacct gggtgcgacc aaacgacttc
    68161 ttaatattgg caatctcaat gctcatgatt ttcctcctgc tgcgcgcgtt tttcctgatt
    68221 ctccaggcgc cactgcaaca tactctttaa aaacagggtg ataatcgcca tcagcgtcaa
    68281 cagcgcggcg gcggtaaagg agccgactgt gttgtagtcc tgctccagca attcaatctg
    68341 taacggcagc gaaagtgttt cgccacgaat cgagccggaa accaccgaca ccgcgccaaa
    68401 ctcgccaatc gcgcgggcgt tggtcagcac tacgccataa agcagcgccc agcggatgtt
    68461 cggcaatgtg acacggcgga acatctgcca gccggatgcg ccaagcaaaa tcgccgcttc
    68521 gtcttcctga ctgccctggc ttaacatcac cggcaccagt tcgcgcacca caaacggaca
    68581 cgtcacgaag atagtgacca gcaccattcc gggccaggag aacataattt gcagattatg
    68641 ttcatccaac cagccgccga gcgggccgtt ggagccgtag aacagcagat acaccagacc
    68701 ggcaaccacc ggtgacacgg caaaaggaat gtccagtagc gtcagcagta actggcgtcc
    68761 ggggaagtta aagcgcgtca ccagccaggc cagcagaatg ccgaacacca gatttaccgg
    68821 cacggcaatc aacgcaatca tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc
    68881 cagattctgt aaaaccggca tcagcccctt gctgaatgcc tgcacgaaga tgtaaatcat
    68941 cggcaccagc aagatgaacg ccgaaaccag catcccgatg ccaatcagaa accatttgcc
    69001 ccagttaatc gggcgcgcgt cataacgctt caactgtgtc acttccgcca ttaatgacct
    69061 accacacgcc gaccaaagcg actttgcaga gtgttaattg agaacagcag cagcagggat
    69121 gccgcgagga tcaccgaagc aatcgcgctc gccgccgggt aatcaaactc ctgcaagcgc
    69181 acaaagatca tcagcgacgt cacttctgtc ttccaggcga tattcccggc gataaaaatc
    69241 accgcgccaa actcaccgag gctgcgggta aacgacagcg ccacacccgc caccagcgcc
    69301 ggagaaagct ccggtaacac cactttgcag aaactctgcc agcgcgtggc accgagcgtt
    69361 tccgccgctt cttcatattc cgggcctaac tcttccagca ccggctgcac ggtacgcacc
    69421 acaaacggaa tgctggtaaa ggccatcgcc accgcaatcc caagccaggt gtaggtgact
    69481 ttgatatcaa acttcgccag ccattcaccg taaaaaccgt ttacggaaaa gagcgaggcc
    69541 agcgttaaac cagcgactgc cgttggcagc gcaaagggta aatccatcag cgcatcaagc
    69601 agcgtgcggc cagggaagcg ataacgggtt aaaatccacg ccatcagcag gccaaaaacg
    69661 ccgttaaaaa tcgatgccac aaacgctgac agcagcgtca ctttgtaggc cgcgaccacc
    69721 tgcgggttgg tgatcacctc ccagtactgc gcccagctca tctgagccag ttgcatcacc
    69781 agcgcggaga gcggcagcag caaaatcagg cacacaaaca gcagactggt gccgaggctt
    69841 aaggtaaagc ccggcagcac acgtctggaa gagacagcaa acatcagtta cgccccgccg
    69901 ctaacagctt gtctaactcg ccgccgctgg tgaagtgggt tttcatcact tccggccagg
    69961 agccaaactt gtcttccacg cggaacaact cggtctgcgg gaatttgtct ttcagtttgt
    70021 ccatgacttc cgggttattc acgcggtagt aatagtcggt gatgatggtt tgcgcctgcg
    70081 ggctgtagag ccagttcagg taggcttttg cggctttttc cgtaccgttg gcctgcacgt
    70141 ttttatcaac ccatgccacc gggaattccg ccagaatgtt ggttttcgga atcaccactt
    70201 caaaaccctg cgcttcatac tgtttacgga tgttgttcac ttccgactcg aagctgatca
    70261 gcacatcacc caggccgcgc tcggcaaaag tggtggtcgc gccacgaccg ccggtatcga
    70321 acacttcaac gtttttcagg aactgagtca taaactgttc ggttttggct ttatcgccac
    70381 cgtcggcttt gtccgctgcg ccccacgcgg ctaaataggt gtaacgcgcg ttacccgacg
    70441 ttttcgggtt cgggaaaatc agcttcacat cggagcgcac caggtcgttc cagtcgtgga
    70501 tattcttcgg gttgccctta cgcaccagga agcccattgt ggagtagaac ggcgagctat
    70561 tattcggcag gcgcgtttgc cagtccgccg ggatcagctt gcctttgtca tgcaggattt
    70621 gtacgtcggt cacctggtta taagtgacaa cgtcggcttt taagccctgc aaaatcgcca
    70681 gcgcctgttt tgatgacccg gcatgagatt gttttatcgt tagtttatcg ccgccgttat
    70741 ctttggccca ttgttgctca aacggcgggt tcagggcggc aaacagctcg cgggagacgt
    70801 cataagaact gttcagcagt tccgttgcct gtacatggcc cgccagcagc agagaagcga
    70861 ccagcgcgag tgagttcttt ttcagtaagt taacggccat tgcgcaccct tatatattta
    70921 atgactttct aatagccatc atatttataa cggacgtgaa aggagtaacg gttttatata
    70981 ccgtttggtg atttggaagt tgaaaaggga ataagaactt ggttcaacag gcgtaattgt
    71041 tgcagtcagt ttggacacga acagcgcgga gaaaccggag cgtacacata gtacgtgagg
    71101 atttcgagca ctgcccgggg ccaaaatgac aaataaaata gcctggtgaa cgtagttcaa
    71161 gacgaaatcc tccccacaaa tgcagggagg aaacagaggt gaatcagata ccgacgctaa
    71221 ccgtctccgg cagtgtgctg ccgccatcaa tcacattctg tgtaccggtt aaatagctgg
    71281 attcatccga tgcgaggaag gccgccagtt cgccaacttc caacggatcg gcgaggcgac
    71341 gcatcgggat tgctttcgcc atttcagtca gcaccgattc tggatcttcc gggttcgact
    71401 ggcgggcaat gctttccgcc attggtgtgc gaacgtatcc agggcatatg gcgttaacgc
    71461 gaataccaga ctgcgcatac tccaccgcca gcgatttagt caggccaaca atcgccgctt
    71521 tcgttaaggc atacgccgtt tcacccggat cggccaccat atcgccagtg accgaagaca
    71581 tcatcacaat gcgaccatct ttgcgggcaa tcatctccgg cagcaccgcc ttcgtgacgt
    71641 tccatacgcc tttaatattg atatcaatat ggaaatcgcg atcttcatcg ctcatatcga
    71701 ggaaactgcc cagacgacaa acgcctgcgt tattcaccag gatatcaatg cgcccttctt
    71761 tttccttcgc gcgtttgata gctgcggcta ccgacaccgg gtcacgcaca tcggcgacaa
    71821 ccgccgtaca gcgatgacca cgaccacaca gttcgtccgc cagcttttcg atctcagggg
    71881 agatatccag caagattagg tttgcgccat gacgcgcaaa agttctggca attccttcgc
    71941 caattccctg caatgcgccc gtaatcagtg ctgtcttgcc cgtgagttta cccattttaa
    72001 tgctctcctt gttaatacag agcgttacac tccattccct ctgaaaatac aggataaaca
    72061 aaatattgct gcggttcagc tcactaattc aggaaaatct caatccagtc gctgctttat
    72121 gcatcatttc aggcgttgtg ttagctcact gctgcgctga atcattttca atgactcgac
    72181 atcattgagt tgcacaaggc caacaaacag taaatcggta acagaatttt gcgccgtgcg
    72241 ggtagacatc gacgagctac gccattcggt ttcgccagaa acggtatcga gggtgtaatg
    72301 cgccagccgc cgcagcgggg aatcggttaa cgaggtaatg gcaatcaccg ttgccccctg
    72361 ctttcgtgct gcctcggcgc acaagacaat ctctttttta ctgccgctgt aagagatagc
    72421 gatttgtaca tcgccttttt tcagcgcctg ggaaacggtg gcttgtacat gtgtgtcggc
    72481 ttcgcaggcc acgcgatagc caattttcat cagtttgaac gacagatcgc gccccaccag
    72541 cgccgacccg cccaggcccg ttatctggat aaacggtgct ttactgatga cctcaatgat
    72601 tttttgcagc cgcgcgaagt caaacaacgc gcaggtttgc tccagcgcca gctgcttttc
    72661 gcgattcagt ttacgggcaa tcacttccag cgaatcgtca ctggtaatcg aactgtgcag
    72721 gtgcagcgcc gtagcgttcg ttttctcccg gctggcgctg tattcgccaa ttaacgccat
    72781 acgcaattca gtaaaccctt gcgcgccgag cttctgggca aacttcacaa tactcgactg
    72841 gctaatgcca agctgtttcg ccatcttgcg cgaagagacg gatttcagct cactcacgtt
    72901 ggcccgcaga aagtcagcaa ttttttgctc gttcccggta aattcacttt ccgcattgcg
    72961 tattttcgtc aggtacaaca tcttccactc tcctgggctg gcagcaaaaa ttccgcttaa
    73021 gttcttatga cgctcttcac actctgcgag tgtagactca atgattcctt taagtcggtc
    73081 aatacgggat atatgattcc atacatatgc aattatttga tcgtaaatag taaggtcacc
    73141 accgatgcaa cttgaaaaga tgattaccga aggctcgaac gccgcttcgg ctgaaattga
    73201 ccgcgtatcg acgctggaaa tgtgccggat tatcaacgat gaagataaaa ccgtaccact
    73261 cgccgttgag cgcgtactgc cggatatcgc cgcggcgatc gatgttattc acgcccaggt
    73321 cagcggcggc ggtcgtctga tttatctcgg tgcgggaaca tccggtcgtc tggggattct
    73381 ggatgccagc gaatgtccgc cgacctacgg cgtgaaaccg ggtctggtcg tgggtttgat
    73441 tgctggcggc gaatatgcca ttcagcacgc ggtggaaggc gcggaagata gccgggaagg
    73501 cggcattaac gatctgaaaa atattaattt aacggcgcag gatgtggtgg ttggcattgc
    73561 tgccagcggt cgcacgccgt atgtgattgc cggactggaa tatgcacgcc agctcggctg
    73621 ccgtacagtg ggaatttcca gtaatccggg gagtgccgtt tcaaccaccg ccgagtttgc
    73681 cattacaccg attgtaggtg ccgaagtggt cactggttct tcgcgaatga aagcaggcac
    73741 agcgcagaaa ctggtgctca atatgctctc caccgggctg atgattaaat ccggcaaagt
    73801 gttcggcaac ctgatggtcg atgtggtcgc caccaacgaa aaactgcacg tgcgccaggt
    73861 caatatcgtc aaaaacgcta ccggatgtag cgcagagcaa gcggaagcgg cgttaattgc
    73921 ctgcgagcgc aactgtaaaa cggccattgt gatggtgctg aaaaatctcg atgccgcaga
    73981 agctaaaaaa cgcctggatc aacacggcgg ctttattcgc aaggttttag acaaggaata
    74041 actcatggcc aaagagatca gcagtgaact tctgaacacc attcttaccc gtgtcggcgg
    74101 accgggaaat atcgccagtt gtggtaactg tatgacgcgc ctgcgtctgg gtgtacatga
    74161 cagttcactg gttgatcccg atatcaaaac gctggaaggc gtgaagggcg tcattttgac
    74221 cagcgatcag gtgcaggtgg tttttgggcc tggtaaagcg catcgtgccg caaaagcgat
    74281 gagcgagctg ctgggagaag caccggtaca ggatgccgca gaaatcgccg cccagaacaa
    74341 acgtcagtta aaagccaaac aaacctccgg cgtgcaacaa tttctcgcca aattcgccac
    74401 catcttcacg ccgcttatcc ccggttttat tgccgccggt ctgctgctgg ggatagcgac
    74461 gttaattgcc acggtgatgc acgttccggc agatgctcag ggcacgctac ccgacgcgct
    74521 gaattttatg aaggtgttca gcaaaggttt gttcaccttc ctggtgattc tggtgggcta
    74581 taacgccgcc caggcatttg gcggcacggg cgtcaatggc gcaattattg ccgcactctt
    74641 tttgctcggt tataaccccg ctgcaaccac cggttactac gccggttttc acgatttctt
    74701 tggtctgccc atcgatccgc gcggcaatat tatcggcgtg ttgattgccg cgtgggcctg
    74761 cgctcgcatt gaaggcatgg tacgccgctt tatgccggac gatctcgaca tgctgctgac
    74821 ctcgttaatc accctactga tcaccgctac gctggcctac ctgatcatta tgccgctggg
    74881 cggctggtta ttcgaaggta tgtcgtggct gtttatgcac ctgaacagta atccgctcgg
    74941 ttgtgcggtt ttagccgggc tgttcctgat cgccgtggta tttggcgtgc atcagggctt
    75001 tattcctgtt tacctcgcgt taatggacag ccagggattc aacagcttat ttcccatcct
    75061 ttcaatggca ggcgcgggcc aggtgggcgc ggcgctggca ctctactggc gggcgcaacc
    75121 gcacagtgca ttacgcagtc aggtacgcgg ggcgattatt cccggtctgc ttggcgttgg
    75181 cgaaccgctg atttatggcg tcaccctgcc ccgcatgaaa ccgtttatta ccgcctgttt
    75241 aggcggcgcg gcgggcggtt tgtttatcgg attgatagcc tggtggggtc tgccgatggg
    75301 cttaaacagc gcctttgggc cgtctggtct ggtggcgctg ccgctgatga cttcctcaca
    75361 aggcatcctt ccggcaatgg cggtttatgc tggcgggatt ctggtggcat gggtttgcgg
    75421 gtttattttc accacgctct ttggctgccg taacgtcaat ctggactgat atcatgaaac
    75481 ggacaatgct ctatctttct ctgctggctg ttagttgtag cgtcagcgcc gcaaaatacc
    75541 ctgttctgac agaaagttcg ccagagaaag cagggttcaa cgtcgaacgg cttaaccaga
    75601 tggatcgctg gattagccag caagtggacg ccggttatcc cggcgtaaac ctgctgatta
    75661 taaaagataa tcagattgtg tatcgcaaag cctggggagc ggcgaaaaag tacgatggca
    75721 gcgtattgat ggaacagcct gtcaaagcca ccaccgggac gctgtatgac ctggcttcca
    75781 ataccaaaat gtacgccact aactttgctc tgcaaaagct aatgagcgaa ggcaagctgc
    75841 atcctgacga tcggattgcg aaatatattc cgggatttgc cgatagtcca aatgacacca
    75901 tcaaaggcaa aaacacgctg cggatttctg acctgctgca tcacagcggc ggtttcccgg
    75961 cggacccaca atatccgaat aaagcggtcg cgggcgcgtt atattcccag gataaaggcc
    76021 agacgctgga gatgatcaag cgcacgccgc tagagtatca gcccggcagc aagcatattt
    76081 acagtgatgt cgattatatg ctgcttggat ttatcgtcga gtcggtcacc ggtcagccgc
    76141 ttgaccgcta tgttgaagag tcgatttatc gcccgctcgg cctgacacat acggtattta
    76201 acccgctgct gaaaggcttt aaaccgcagc aaattgccgc cacggaattg aacggcaata
    76261 cccgcgatgg agtgatccac ttcccgaata tccgcacctc cactctctgg ggtcaggtgc
    76321 acgatgaaaa agccttttat tcgatgggcg gcgtttccgg gcacgcaggt ttgttttcca
    76381 ataccggcga tattgcggtg ttaatgcaaa cgatgctgaa cggcgggggc tatggcgatg
    76441 tgcagctgtt cagtgcggaa acggtgaaga tgttcaccac cagctctaag gaagatgcca
    76501 cttttggcct cggctggcgc gtgaatggta atgccaccat gacgccgacg tttggcacgc
    76561 tggcaagccc gcaaacctac ggtcacactg gctggaccgg aacggtgacc gttatcgatc
    76621 cggtgaatca tatggcgatt gtgatgttaa gcaacaagcc acattcgccg gttgccgatc
    76681 cgcaaaagaa tcccaatatg ttcgaaagcg gtcagctgcc gattgcaacg tatggttggg
    76741 tagttgatca ggtgtatgcg gcgttaaagc aaaagtaata aaaaggcctg tcagtaatgt
    76801 tactgcctgg cctttaatca atgaatcaga aacgcttaca gcgccatcaa cttgtccagc
    76861 gacggtgcga aataatagcc gccggttacc ggtttggtga aacgcaacat cgcatcacgc
    76921 ttaccatcgg tatcgccaaa catgctcagc agttgctgct caatgttatg cagacgcgcg
    76981 cagtaggcgc agaagtacag accgtgcgta ccactggcgg tgccgtacgg caggctctga
    77041 cgaacaattt tcagcccttt gccatcttct ttcagatcaa cgcgggtcag gtgagaagtt
    77101 tccgggcgtt cgtcgccgtc gatctcttcg ttggcctctt tggtgcgccc gatcatcatc
    77161 tcctgatcgt gaacgctcat acggttgagc tgcttcaggt tgtgctccca acgctggaca
    77221 aacacatagc tgccgcccgc atccacgccg tctttgataa ccgccacttc gcgacgcgtc
    77281 tcttcacccg ccgggttttc cgtaccgtca acaaagccgc tcaggtcacg ctcttcaacc
    77341 caacggaaac cgtggatctc ttctttcact tcaatgcagt cgccgaaagc ttccatcgcc
    77401 gcctgagcga cagagaagtt tacgtcgtga cgcaaagaaa gaatgtggat caacacatcg
    77461 aactgggtcg ttggcgcaag gcctttaccg tagcccggaa aatctttcag ctcttcggcc
    77521 ccaacgccgc cgctcagagc gcgccaggtg ttgttaccaa aggcaaccac cgcaccaaga
    77581 tgcgcgtccg ggaatttcgc ttcaaaagtc gccagtttgt cggcaaatgt tttactggcc
    77641 gcacgcaggg cgtcaacttc ccctttcacg ttggcttcga tccaaatcgc cgcgcggcaa
    77701 tgttctggca aaatgccact ctgaacctga gacattgttc ctcctgaaaa taataatgcc
    77761 acgcaatccg tggcattgtt gagcgctatt ttacgtgttt tttcgcggcg gcggtttgtt
    77821 cagacgcaaa ttaacgccgc cagataattt tactgacttt ccagtttttc agggtatcgt
    77881 ccgaaggcat taacccttca ggaccactcc attccccgct gaactggtag ctaatatgtt
    77941 gactgccttc ggctttacat tcgacggcac gattatcatc accatcagct ttttggcaat
    78001 tgccaaatgc tttgctgtaa aggtcgctaa acggtgtacc gattttaaca ccggtgtcag
    78061 caggaatatc gctatccagc acgtcaatgc gactgatcgt gccttgatcg ccgttaatca
    78121 ccatcgccac gttgtcgccc ttcatcactt caaaaaagcg caccacgttg ccgttcgcgg
    78181 ttttcattcc gctgcgcagg cgataatcgc catcaagcgc atcggcaatg gcttgttctt
    78241 gcagtggtgt ggacgccgtt aattcaccaa cgccctgctc gctcactttg gtggacgacc
    78301 caaaccagtt ccacgggtta gcggcagacc agttaactga cgacatcgtg gaacagccgg
    78361 tcagcatcaa tggcatagcg cataacatta aacgcagcga tttcatgtca cttcctttgg
    78421 ttattcaata acgttgcttg gagtgcaaat tcaccaaaaa gtgccgttat tcttttactt
    78481 cattaaaaca agcgcgtaaa cgtcgattgg tcagcagcca gattaacgcc acaatatccg
    78541 ccactaccag cgccagaccg aaaccgctaa cggattcacc gttcagccac agccacggtt
    78601 gccagcaaag taaaaccacc tgcgccaaca gcaacagaaa atagagtaca cgccaggtgc
    78661 gaggaaacgt agcccgccga ccgctcaaca aaaacgccag caccgccgga atgccaggaa
    78721 tcagccccag ccagaaatta tcgtgatcgg gataaaacag atttagcagc gcagtaccct
    78781 gctcgcgcga cgcaccggca atgacaaaca gcacccaggt tcgcgcctga agcaatagca
    78841 caagccagaa gagcaagggt aaacgcaggc gaccgtgcgc atcataatgg acaggatgaa
    78901 actcagtact cttcatcttc aatcaaacgc ttacccagac tcagcacgtc ggcgtgttca
    78961 tatcccaggc gttcatacat tccgagcacc atgtcgttat cttccggcac gttgatctga
    79021 attttcgggc agccgcgagc aatcagcttt ttctccagcc ggttaagcaa cgcattggca
    79081 atcccacgcc cacgaaactc tggatgcacg ccaagataat aagcagaccc gcgatgcccg
    79141 tcataaccgc ccatcaccgt tccgaccact tcaccgttca cctcagcgac cagaaacaaa
    79201 ctgacgtcat ggttcatctt acgctcgatg tccatttccg gatcgttcca cggacgcagc
    79261 aagtcgcaac gctcccaaag ggtgatgacc tcttcgaaat cttcctggcg aaatacgcgt
    79321 atctccatgg tattcgttac ctttttgcgg gttaaaaggc tgattatggc gtgaacggtc
    79381 gaattagcca atatctgacg aaaatcggtt gaaaaagtgg cataatgggg agttgtcaac
    79441 tattgaaatg aaaagtaaaa caattctcaa cagcaaacgg tcgtaacgga ttacgcgata
    79501 cgatataaca tctggaactt tattattaca actcaggccg tatgagcact tttaaaccac
    79561 taaaaacact cacttcgcgc cgccaggtgc tgaaagccgg tttggctgcc ctgacgttgt
    79621 caggaatgtc gcaagccatc gccaaagacg aacctttaaa aaccagcaac ggacacagca
    79681 agccgaaagc taaaaaatct ggcggcaaac gtgtcgttgt tctcgatcca ggccacggcg
    79741 ggattgatac cggagcgatc gggcgcaacg gttcgaaaga aaaacatgtg gtgctggcga
    79801 ttgctaaaaa cgtccgttcc attttgcgta atcatgggat tgatgcgcgt ttaacgcgtt
    79861 ctggcgatac gtttatccca ctttacgatc gcgttgaaat cgcccataaa catggcgcag
    79921 atctgtttat gtcaattcat gccgatggct ttaccaaccc gaaagctgcc ggtgcttcgg
    79981 tatttgccct ctctaaccgt ggggcaagta gcgcaatggc gaaatacctg tctgaacgcg
    80041 aaaaccgcgc cgatgaagtt gccggtaaaa aggcgactga caaggatcac ctattgcagc
    80101 aagtgctgtt tgatctggtg caaaccgata ctattaaaaa cagcctgacg cttggctcgc
    80161 atattctgaa gaagattaag ccggtgcata aactgcacag ccgcaacacc gaacaagccg
    80221 cgtttgtggt gttgaaatcg ccgtcggttc cttcggtgct ggtggaaacc tcgtttatca
    80281 ccaacccgga agaagagcgg ctgttaggca cggcggcgtt tcgtcagaaa atcgccacag
    80341 cgattgctga aggcgtgatc agttatttcc actggttcga caaccagaaa gcacattcga
    80401 aaaagcgata agttatgaaa cccgacgcac accaggttaa acagtttctg ctcaaccttc
    80461 aggatacgat ttgtcagcag ttgaccgccg tcgatggcac agaatttgtc gaagatagtt
    80521 ggcagcgcga aggtggcggc ggcgggcgta gtcgggtgtt gcgtaatggt ggtgttttcg
    80581 aacaggcggg cgtcaacttt tctcacgtcc acggtgaggc gatgcctgct tccgccaccg
    80641 ctcatcgccc ggaacttgcc gggcgcagtt tcgaggcgat gggcgtttca ctggtagtgc
    80701 atccgcataa cccgtatgtt cccaccagcc acgcgaatgt gcgctttttt attgccgaaa
    80761 aaccgggtgc agagccagtc tggtggtttg gcggcggctt cgatttaacc cctttctatg
    80821 gttttgaaga agatgccatt cactggcacc gcaccgcccg cgacctgtgc ctgccgtttg
    80881 gcgaagacgt ttatccccgt tacaaaaagt ggtgcgacga atacttctac cttaaacatc
    80941 gcaaagaaca gcgcggcatt ggcgggctgt tctttgatga tctgaacacg ccagatttcg
    81001 accactgttt tgcctttatg caggcggtag gcaaaggcta caccgacgct tatttaccaa
    81061 ttgtagagcg acggaaagcg atggcctacg gcgagcgcga gcgcaatttt cagctctacc
    81121 gtcgcggtcg ttatgtcgag ttcaatctgg tctgggatcg cggcactctg tttggcctgc
    81181 aaactggcgg gcgcaccgag tcgatcctga tgtcaatgcc gccactggta cgctgggagt
    81241 atgattatca gccaaaagat ggcagcccag aagcggcgtt aagtgagttt attaaggtca
    81301 gggattgggt gtaactccct cacccccatt cccgcatccg ctgatgcaac gtcaacgacg
    81361 gcttctcggc aaacagctgc tggtaatccg tggcaaattg ccccagatgc cagaatcccc
    81421 actgcatggc ggcgtctttt accgttgtgc tttgcgacca cgggcttatc agttcgcggc
    81481 gtacggcgtt caggcgaatg cgtttcagcc acgcgttcgg gccaatgcct aaaatagcgt
    81541 gaaacgcgtt ttgtagcgtg cggcggctga catgcagttg attacacaag tccagcaccg
    81601 tcaccggttc ggacatgttt tccagcacat attcacgggc gcgggaaagt aatctacggt
    81661 aactctgatg actgatgctt tccgccgtca ccattggttg cgcttcttcc agcatcgccc
    81721 ccatcgccat tagcaaatta tcccccagca cttttcgcac tgcaggctga tggagatttt
    81781 ccgggttctc gcaaaacgtc gccagcgcct gttggacaaa gccccacagc gcggctttat
    81841 gctgctcttt cacttccagc gccgattggc tacgcagcat atgtaatacc cgatccggat
    81901 tgtgcaaaaa gttagcctga cgggtgatga catcttcaga cagcactacg cccaggatcg
    81961 tgtaatcgtc cggcgtactt aattcaaact cggttccacc ggggcgcgtc gctatttctg
    82021 cgcttcccag acattgcgaa ccgataaatc cctgctcacc gcgcgtcgcc ggaatgccaa
    82081 accagaacga gttaggccag accaggcacg actgacgcag cgccagaccg gtgtattcac
    82141 gaaaaacctg aatatcatcg agtagaattt ccgtaaattc accatgaaac ttgcccggat
    82201 gcagctgatc gtaaatctgc tgccaggcgg taatcgttaa agcatgttca tagacatccg
    82261 ttgtctgtcg ttgatgaaca ttatccactt cgaccttcgg cgtgagcttc aggttttcgg
    82321 gtaagggttc atgataaaga tggtgcaaat tggctgtacg ggtctttttc atgatgttaa
    82381 tgccgggtgt tgtaggacac ccggcacctc cgacaggtta atggggcttg agacgataac
    82441 gactactgcg tttacgtaac gtcccggcag aaaagagctg ttcgagcgca tttctggctt
    82501 tttccagcgg ccagccaaag tgggcagcca cttctcctgc cgtcattccc tgacgtactg
    82561 atgttaacag cgccaacaac tcgtcagcag attccgcgac gataactggc gcgtcgggtt
    82621 cctttacggg ttgcttcggc tggcggttaa agccagtaac cagccactgg gtgtcatcgt
    82681 ctggccgacc gatctccttg cggctgatga cacgcccggt acgcatcgcg gcagcacaac
    82741 ctgcgtccag cgcagcacga cacgccgcca gatcgccttc caccaccagc gttaagcgac
    82801 cagggtcaag cacttcgtga ctgagcagac gaacgttagc tgctttgagc atggcatccg
    82861 ctgcatctat cgcagcgacc atgccgtcca cttccagcaa tcccagtgca ttgatcatcg
    82921 gcaacctcca ttacgcacgc tggattggat tacgcgcgat ttccagcact gcatcggtaa
    82981 aggcgttaca ggccgcttta cacgctgcct ggctaccggt taaaaatgct gccgagtagt
    83041 tagtttcaga cggtggcggg acataggtca ccagctgtac gtcggcagat ttcaacgctg
    83101 catcaatgcc gtaggtcgct tccagcggcg gtgccaccag atacgccatc ggatcgccaa
    83161 gcgtgatccc ggcggttgat gagagataag aaccggtacg cgaaactaca tgtgccagga
    83221 atgccgtatc ttgcgcatca ttagcccact ggaaagcagc gccattttca atatgcgcaa
    83281 ccatcgcatc cagaccggca cgcacttccg ccgggtttgg cccaccaagc ataatcagca
    83341 cttcaccggc agtcggtgac gggccgtgtg cagcgcccgc atacagcgag cggccataca
    83401 ccacttccac catcgcctgc ttggtcgctt catcagcggc aatgtaggtg acgtcatcgg
    83461 agtcggcaga aatcagaccg aggctacgaa tatgcggcgg caatttcagt tcacgcgcaa
    83521 aatcggcgtt aacggaggca atcacccgca tggcggttac cgacggtcga atcaaatcta
    83581 aagctggcat gatgcctcct tatcgggtca tgttgatgcc ggatgctttc tgctccagca
    83641 tacgtttcgc caaatccaca atgacggctg cggcttcaac cggcggcgtg ccgccctggt
    83701 gaatgttaga gatgcaggtg cgatcggcct caacggtggt cgccatacgc ggcgagtaaa
    83761 cggcgtagca ggagaggctt tctgactgac ccaaccccgg acgttcgccc accagcagga
    83821 tcactacttt cgcgccgagg atctcaccga tctgatcttc aatcttcacg cgaccataac
    83881 gaacaaagaa tggtgtaccg actttcagac cggcctgttt caggcccgcc atcagcggtg
    83941 gcaggatctc ttcgtagttc acggtgatcg catcagttga caggccatca gaaataacga
    84001 cctgcacgtc cggattggca acacactgcg ctttcagcgc ttcaacagct tctgcacaca
    84061 ggcggcggcc catatccggg cgagtcaggt agagattttt gtcgctaatt tcggagcgga
    84121 cttccagcaa gccttgtgct ttcacccact cttccggcac ttctttcagt acggtatctt
    84181 tcgaacgaga gtgatcggcc aggaaacgca gcagcgcctg cgtacgtgga cgcggaccgg
    84241 cacgaccggt acaaacgcgg gccacggtgc tgcggcgcag ttctgttaat acgtctgcgc
    84301 gatgcgggtt ttcaacacca atccacgctt ttgcttccgc agaacctaaa tccagcgcgc
    84361 agctttccga ggtcaccggt gccgcacagg tggtggtggt gcactttgct tctgacggcg
    84421 cgggggccgt ttgtcccatt gacgccatca cgctgcgtac aatttcttca atctgttttt
    84481 gatccatgat gtgttatctc cgcgtcatca gaagaacagt gacggatcgc ccgcccgttt
    84541 ggtcaggcga ccgtttgcca taatgcccat gctttccagc cagcgttcaa actccggtga
    84601 cggacgcagg ttgagtaact gacgcacagt ggcggtatcg tggaatgcgg tggtctggta
    84661 gttgagcatg atgtcatcgc ccaacggcat ccccatgatg tagttgcagc ctgcggtggc
    84721 gagcaggatc atcaggtttt cgttgaggtt ctggtcagcg tcagcgtggt tggtgtaaca
    84781 gcagtcacag cccatagaga tgccgctcag cttgcccata aagtgatctt ctaagcccgc
    84841 acggataatc tggcggtcgt tgtagagata ctccggccca ataaaaccga ccacggtgtt
    84901 gacgataaac ggatcgtaat gacgcgccag cccatagtta cgtgcttcca tcgtcacctg
    84961 gtcagcgccg aagttagcgc cagcggatag cgcagagcct tgtccggttt cgaagtagag
    85021 gcagttttcc ccggcgatac ggttgaactc tgcgcccact gcgcgcgctt cgtcgagcat
    85081 cgccagttcc acgccaaact ctttcagccc tttttcgctg ccacagatac tctggaaaat
    85141 cagtccgcca ggcgcgccgc gacgaatcgc ttcgatctgg gtggtgacgt gcgccagtac
    85201 gcagccctga gttgggatgt tgaatttgtc gatcacgcca taaatggtat ccagcacgcg
    85261 gcttaagttt tccacatcgt cagtcaccgg gttaacgccg atcaccgcat cgcccacccc
    85321 gaaggaaagc ccttcgtaga tttgcgcggc gatactttgc acgtcgtcac gggtgtcatt
    85381 tggctgcaaa cgggcgctaa aggtgcccgg aataccgatg gtggtattgg cctttttgat
    85441 taccggcatt ttcttcgcgc cgtagatcag gtccgcgttg gagcaaatct tcgctaccgc
    85501 cgcgaccact tccgaggtca gccctttgcg ggtaaaggca atgtcgtcca cgctggtttc
    85561 atcgctcagc acatactcac gcagttcgct gatgctccag tttttaatct ggttgtaggc
    85621 cgtttcgtta acatcgtcct gaatcagccg cgtcacgcag tcatcttcat aggcaatcac
    85681 cggattattg cggatgtccg ctacggtcat ttccgacaac acctgctttg ccgccacgcg
    85741 ctcctgtgag cttgccgctg cgacgcccgc cagcacatcc cccgaacgca gttcgttggc
    85801 tttagccagc acctctttta catccttaaa ctgatataca ttgccgaaca atgtggtctt
    85861 tagtttcata agtcgttccc tcaggaagga aatgcgagtg atttcaccgt caccggcaca
    85921 accgatccgc caaaaagagg cgtaccaatg tcgatatagt cccccgcgcg gacaatcact
    85981 tcgtcaatga ctgccaacgg gagttgttgt agctgtgggc gcaacaacat gcccagagct
    86041 ttaccaaagt cctgcccggc caccaccagc aggggatgcg gattcggaaa acgcgcgacg
    86101 aaatcgacca acgcgttgat gaccgtcagt accgcggcgt aacgcacagg cagcgaggcg
    86161 ggaagcgcca gcacgtacgc gtcagttttg ggatcaagat ccagctgaag cagcgcctgt
    86221 tgccaggcac tcaccagatc cgtttcatca attgggatcg ccaccggcaa attgcgcagc
    86281 ggcagttgta cgccctcaag ccagattgtg ctgccagaga gcgaaagggt atgtgcaccc
    86341 gcaccaatca ccgtggcgcg cacggtttgc gccggaaact gtacgttcat ctcacgcagg
    86401 cgcggatggt catgcagcgc cgttgccagc agcgggccaa tatcggcaaa acagaacggg
    86461 tcggcgggct ggtggcgata acattcgccc acgccgccag aaagcgtaat gatttcgggc
    86521 gtaacacctg cgggcagcaa accggtttgc attaatgcct gcgcgagcgg cgaaagcgtt
    86581 ccgtcaatca cttcgacaat cagctctgcc atccgccggg taacctgcac cagctgagcg
    86641 ccggtcagcg aacgggcgtc ggtgcctgcg ccgaagcact catccacaat catctgcccc
    86701 ggtttatgag cgtaaaccac gcgcccctgg ctgtcggttt ccagcaggcg accaccgacg
    86761 ttgaggcagg cagtgccgct gatttttccg gcatcgaaca gggcgtagtt cgcggtgcca
    86821 ccgccgatgt cgatattcag tacccgacac agccgttgtt cagaaagggt ttgtgccccg
    86881 gctccgtgac cggcgatcac ggattcgagg tgcggcccgg cgctggcaac gacaaaatcg
    86941 cccagcgact gagagagcgc catcaccgcc gggcgagcat tgcgggtttt cgcgctttca
    87001 ccggtgatga tgatggcacc agaatcaacg ctttccggcg caatgcccgc agcctgatat
    87061 tgctctaaaa tcagcgattt cagttccgct tcttttaaac caccctgttt atcgacaggg
    87121 gtaaagaaca ccgggctttg ccagctaatt tcgcgtttga tgaattcgta gcgcggcacc
    87181 tgcgacaccg ccgcacggtt aaccagttcc aggtgggaga aaatcacctg ggtggtggtg
    87241 gtgccgatat cgataccgac gctcagtagc tggcgagtgt tcacgattgt gcctccgctt
    87301 cggttttagc cgcggtcgcg tcgtcttttg gcaccagcat catcgccacg ccaatcgccg
    87361 ttacgccgcc gatcaacttg ccgacaatca tcgggaagat cattgcattc atgttggcag
    87421 cggcgaagcc taagtggtcg cccagggcga aagcagcgga aacggcgaag gcgcagttga
    87481 tgactttgcc gcgggtatcc atctgcttca tcatgccgaa catcgggatg ttgttggcaa
    87541 gcgttgccac catgccggct gccgcgatgt tgttcatatt cagcacttta ccgacgctca
    87601 tcagcggttt ttcaaaccag cgggtcagca gcagcaccat cggatacgcc cctaacagaa
    87661 cgcaggagat agaaccgata acttcaatgg cgcgcatcac ctcaccgggt ttatcgccag
    87721 gggccataaa gataggatcc agaccgggga tcagttccca gccaagcagg aatttcacta
    87781 ccgcagcggc aagaccgagg gtgatcaatg caacgaggaa tttggcgaag atctggaagc
    87841 cgttgatcat tttttccggg atgaatttca gccccagcgc caccagaatc gcaacaatga
    87901 tcaccgggat catgttcatc aggatcaggg cgaaagtgaa ttccaccggc tggccgttga
    87961 tctgcacacc ggagtacata gctaccagac caccagcaat acagccaatc ggaatggtca
    88021 caatgcccgc cagcacgccg agcgccagat aacgacggtc agaaggttcg ataatgccga
    88081 gcgccaccgg aatggaaaac acaatcgttg gccccatcat cgacccgaga attaaccctg
    88141 agtatagcca cgctgctacg tcgccgcccg ccagctcttt ggcgaggaag aagccgccca
    88201 tatcgcacgc cagcagcgtt ccggcgaaca ttgacgggtt tgcgccgagc atttcgtaaa
    88261 ccgggataat taccggcccg agtatgtgag ccagtaccgg tgccagcgcg gtcataccga
    88321 ccatcgccag gcccagtgcg cccattgcca taaagccttc ttcgaactga ccgcctgatc
    88381 cttcgatact tttaccgaac ttaccgagga aacgagcaga accgccgaac tgtgacagga
    88441 tcctgtctac ggcagctatc agcataaaga acatcatgat gtacatgatg atttcgttaa
    88501 ttcccatcgc ctttactccc tgttagttgt tatttattag cggatgctgc gtgaacgcct
    88561 tatccgccct acatgtgcaa tcccgtaggc cggataagac gcggcaagcg tcgcatccgg
    88621 caattgcacc gcgccactgg cggatgcggc gtgaacgctt tatccgctct acatgtgcaa
    88681 tcccgtaggc cggataagac gcggcaagcg tcgcatccgg catttattgc gccgctgcgt
    88741 acaggcagac aatctgctcc aggctggcgg tacgcgggtt gctgcgcaga caaatatctt
    88801 cctgcgcggc ctgcgcccat gcaccgtaat gcgcagatgt cgcgccaaca tcgcccagtc
    88861 gtttaccaat cccaacttcc gcaatcagct cacttaccgc gttaatagcg tcacgatcgt
    88921 cggatttttt agttcgcagt gcccgaccaa tctgactaaa gcgttcgcga caaaccatcc
    88981 ggttaaactc catcaccgtt ggcagcaaca tagcgttcgc cagaccgtgc ggaatatgca
    89041 gcgccgcgcc cggctgatgc gccatcgcgt ggcacaaccc aagacccgca ctggaaaacg
    89101 ccattcccgc catacatgaa gccagcaaca tgctctcgcg cgcggcaagg tcgtgaccgt
    89161 agcccaccgc tttcggcagc gatttgccaa tcatcgcaat ggcaccaatc gccagactgt
    89221 cggtaaacgg tgtagcgttc agggcgctgt atgcttcaat ggcatgggtt aacgcatcaa
    89281 tgccggtcat cgccgtgaca tgcgacggca caccttcggt caatgcggcg tcgaggatcg
    89341 ccacatccgg catcagcgag gcatgggcta acacctgctt gcgcccgctc accgcgtcga
    89401 taatcaccgt tacatttgtg gtttcagagc cggttccggc ggtagttgga atggcaatca
    89461 gcggcaagcg cggttgcaga acgctggttt ctgacatctc tgccagcgtg ctatccgggt
    89521 tcgtcaccag caacgccacg gctttcgccg catccagcac cgagccgccg ccaaatgcga
    89581 tcaccccgtc acaacctgat tcacgcaact gcgccacggc tgcacacacg tcggtaatgc
    89641 acggttcgcc caccggacat ggccagagcg tcatggcgat acctttaacg gccaggctac
    89701 gcgtcagccc ggcggtcatc cctgcctgat gcaaaaagct gtctgccatc acgaacagat
    89761 gtttcagccc acgcgtttgc gcttgctgtc cgcaactgct caccgagccc ggaccacaaa
    89821 gcgtcaccgg tggaacacta aatgttttta cccgttgcag attcagggta tcgaacgcct
    89881 gaaagagcgc ggtctgcaat tcattttgca tatagcccct ccgctttctc tcgcccacta
    89941 ctggcgatcg ccagcggggt cataaacaaa ctgtgctgcg gtaaatgcac ctgtaacgcc
    90001 gggaattgtt tgcgaaacag ttctgccacg cccggttgca tacaagagcc gcccgccagc
    90061 cataaatcag taatgccctg cccttcaata tgccgggcga caatgtccgc cattttttcg
    90121 tacaccggtt tcaccgcggg ccaaatctcg tcaccgtgac cgcgcttgta ctgctctgcc
    90181 tcttccagcg gaatacgacg atttccggcg agagtcagag agatgtgatg tccgccagtc
    90241 gcttcatccg ccgagtacgt caccttgccc tttttcacga tggcaatgcc ggtagtgccg
    90301 ccgccgatat ccactacacc ggcgttgtcc agttgcagca gatccgcgac tgcggttggc
    90361 tcatccagca catggctcac ttccagtccg gcagattcca gcacgttgat ggaaatacgc
    90421 gggtccgtac ctggaggaaa tgaagtcgcc gcatggctaa aacgacgacc gaattgctgc
    90481 tcgagcgtgt cgagatggcg acgaacaatg gtgaccgcgc cgaagaaatc ccagacgatg
    90541 ccgtcgcgaa ccacatcggc ccagtcgagg cataccgcca ctggctgacc gtcgcggtcg
    90601 acaaccatcg acaccacatc gcaggtgccc agatccaccc ccagccacag cggagattcc
    90661 gtcgcggcgg gcgtctggtt acacagcgtt gccgccgttt gcagacgtgg ggtgagccat
    90721 tgttcgtcgt gcgccatctg ttactcctta aacaatgcga aacgcatcga ctaatacaca
    90781 gcgacgcaga cggacaaacg tccgcgcgct ggttacccct tcaccggttg gcgtggtgat
    90841 ggtcatggta gtccagcctt ccccgcccag acccagcccg gcaatgcacg gtccgttctt
    90901 aacgaaaatg ctggtatcaa tggcattcgc catctggttc atgttctcga tattgcgcga
    90961 gtgcattgcc gccgtgtggt ggcaaccgcc ctccagtttc accgctagcg caatggcatc
    91021 cgccacgttg gcgacgcgca cgacgggcaa caccggcatc atcagttcag tcacggcaaa
    91081 cggatgttct gcggtggttt ccacaaacag caggcgcgtt tcttgcggaa ctttaaggcc
    91141 gattgccgcc gcgattttcg ccgcatcgcg accaacccag tcacggctga cggtgccttt
    91201 tccgcgctcg tcgatatttt tcagcaacac cggttgcagc tgctgcgcct gttctgcggt
    91261 cagtttcacc gcgtgctggc cttccatcag acgcatcagt tcatcggcta cgctatcaac
    91321 aacaatcagt accttttcgt cggcacaaat gatgttgtta tcgaaagacg cgcctttgac
    91381 gatggactga gcagcacggg cgaggtcagc ggtttcatcc accactaccg gcgggttgcc
    91441 agcgcctgcg gcaatcagac gtttattagt gtgtttacgc gccgcttcta ctaccgcttc
    91501 gccgccggtc accaccagca ggccgatacc cggaaacttg aacaagcgtt gcgcggtttc
    91561 gatatccgga tttgccacag taaccagtaa gttttccggc ccgcctgcgg caacaatcgc
    91621 ctggttgagc agcgtgatcg cccgctggga gacttttttc gccgccggat gcggggcaaa
    91681 aatgacgctg ttgcccgcgg caatcaggct gatggcgtta ttaattacgg ttgccgccgg
    91741 gttagtggac ggcgtcaccg aagccaccac gccccagggc gcgttttcaa ttaaagtcag
    91801 gccgttgtcg cccgtcagca cttgcggaga gagacactca acgcctggcg tgccgcgtgc
    91861 ctgagcgacg tttttggcaa atttatcttc aacgcgaccc atgccggttt cactgacggc
    91921 aagttccgct aaatctctgg cgtgtttttc gcctgcttca cgaatggcag caatggctaa
    91981 ctggcgcatt gccacgctct ttaacccttg ctgggcgact ttggctgccg caacagcgtc
    92041 atccagggac gcgaaaacgc ccatctcatg aacggcggcg ggcggcgtgt cactgctttg
    92101 cattttcagc agtaccgctt tcaccacctg ttcaatatcc tgttgattca tgatgttctg
    92161 ccttatttgt ggaaaattac ctgaccgcca gacaccacct catcgacgat gccaatcacg
    92221 cacagatcga ccggtgacgt ttcgctttta tgcgcctggc gggcggaact gccactcacc
    92281 agcaacaccc actccccggt tcccgcgcca atattgtcga tggcgacggc acattgcccg
    92341 tcaggattac catgtggatc aatcatttcc accatcagca atttgtcatg cgccagtccg
    92401 tgatggcgta cggtacaaac aatttgtcca gtgacgactg ccagtttcat acccgcctcc
    92461 gtggcgtatt tcaggtaaaa gctcccccta ccctccgcgg aaggtaaaat gaaaaatgag
    92521 agagcgtgac gcccgaatcg acgtcacaca gggtgattac aggttgctgc tatcgccttt
    92581 caggccgatc gggaacactt cttccaggtc gccgtgcgga cgcgggataa cgtgtacaga
    92641 taccagctcg ccgatacgct gtgccgctgc ggcacctgca tcggtcgccg ctttacaggc
    92701 tgcaacatca ccacgaacca tggcagtaca caggccgccg ccaatctgct taacaccaac
    92761 cagcttgacg cgcgcagctt tcaccatggc gtcagaagcc tcaatcagtg caaccaggcc
    92821 ccgggtttcg atcattccta atgcttccat tgtgctttcc tctttatcag ggtccagaac
    92881 gggaccgttc attcaaccag tgtttgtaaa ctgctttcgc ggttcacttc tgtctgacgc
    92941 ggcacagctg ccaccagcgc cagctcgata atttcctgca cgctacaacc acgagagaga
    93001 tcgtgcatcg gcgcggcaag tccttgtatc aatggcccga cggcacgata tccgccaagt
    93061 cgctgtgcga ttttgtaacc aatatttccg gcttccagcg acggaaaaac catcacattg
    93121 gccttgccct gtagcgggct ggcaggcgct ttttgcgccg ccacttccgg cacgaaggcg
    93181 gcgtcaaact gcaactcgcc atcgacaacc agctttggtg cgcgctcacg gacgatttct
    93241 gtcgcctgct ggacgttggc aacgcagggg tgacgggcgc taccgttact ggaaaacgac
    93301 agcatcgcca cgcgcggctc ttctccggtg atggcgcgcc aggtttcggc gctggcaagc
    93361 gcgatatccg ccagctgcgc cgccgtcggc tgtggtacca cgctgcaatc ggcaaagccc
    93421 aacgcggggc cactgtactg tggcagcatc aggaaaatgg atgagagcgt tttacagcct
    93481 ggctgcaagc cgataatgcg taatccggca cgcagcacat tcgccgtgga agagaggttg
    93541 cccgcgatac agacatccgc tataccggcg ctgaccattg cggcggcgaa catcagcggt
    93601 tcggtgagtt tttccagcgc atccggcggc gttttttcgc ccgcgcgggc cagccagcga
    93661 tgagcaaatt cttcccgcat tgcgaggttg ccatgcggat caatcacctg tagcccgtcc
    93721 atcgctacgc catgactgag cgcaaactga cgaagttcaa acggattggc gaccagaatg
    93781 ggcgttgcca gaccttgttg atgtaaatat tgcgccgctt tcagcacacg ttgatctaac
    93841 gcatccggaa aaaccactct ggcgggcgct cgcaacgcca gttcacgaca acgttcaata
    93901 atcattgcgt ttcccccagt cgttgttgga tctgcttcac cgttagcggc tgcttcgtta
    93961 cgctcaggat catcatcacg tagaccgtgc tcgaaaggcg gttcagcgcc tgcaagatgt
    94021 ccgggcgtaa tacttcaaag ctgcgggtga taaacacctg cgccgctacg gtttcggttt
    94081 cccgcacttt ggtgcgtagc agattgagca acgccgcatc gcgcccgtgg ctggcttccg
    94141 gcaccagatg atcgtgacca agataacgca acggctgatg agaaagccgg tgcagatctt
    94201 cgtcagaaag cccgacaatc gcctggcagc ccagcggttc ccccagcgca tcggcgcgca
    94261 taatgttgcc aagacgcgaa cggatatccg ccagccacgg ctgccacggt tccgccagtt
    94321 caatttgcag ccacactgcc agcgcaatgg tgctgtcgag aaccgcgcga aaacccagac
    94381 gcggatcgct tttagcgacc attttttccg ccgacagatg agtcagcgta tccggctttt
    94441 ttgccaccgg ctggcggcac agttcgcagc acgcctgcgg atgttcatcg ctactggtca
    94501 gcccgtgaac gggctgtggc tgctgctgct catcgtcaac aaacaggcgg ccctgctcgt
    94561 caataaactt gatgcgcaga tggcggcttt ccagtaactc ccgggcagag ggcgtcaggc
    94621 gactgtccgc agggagatgg atctctgcgc cttcgctgag cgtatggttc gctcttagcc
    94681 atgcttcggt gatgaaatct ttcataggga ttgccagtta gccggccagg cgacatacag
    94741 gaatttcacg ctggatgtcg taccaaattc gatgctggag cctttcggga taaacatcac
    94801 atcgcccgct ttggcgatca tggtttctcc tgcgtggcga acatgcagct ccccttccag
    94861 caccatatcg atttcgtcgt agttcagggt ccacgggaag aatgcgttgt cccactgcat
    94921 aaaaccggcg gccatgctgc tgccgtcgtc tccggtcaca aggtcagtta agcccacgca
    94981 atgcggctct gcgccgtcga aacgaccaaa cttgacgctg ctaccatcaa tcactttgat
    95041 gccgcctttg ccggtcaccg atttgaagct cggctgcatt gcaccctgct ccagcgactg
    95101 tttttccttc atcacttttt ccatcaactg cgcgaccagg ctttcggtaa actggccttc
    95161 cggcagctgg gcaatgatgg tttcgcggat gcgctgactt tcggttttat cggctggcac
    95221 gctggcggga acggacgccg ttaccgggga cgattcatca cattcggtga tggtaaaacc
    95281 cagtagatcc gccacttcac gggcttccgg ggtaataatg ctggcgcgca gaaccacgga
    95341 cattgcctgt tcgccgcgcg cgtgtgcttc acgaatatca ttcgctgtga tgagtttttt
    95401 cacctgcttc ctcctgtttg gtgagagatg ccagataatc caccagttgc tgcacgcttt
    95461 gcgggtcgtg gctgtttagc tcgaaaatcg gctcctcgaa gcccgtctca agcaataact
    95521 ttcgcgttgc ggcgacgtca gcatccggca tgtccgtttt actgatgacg gcgatttgcc
    95581 gtttgctgac gccaatatcc agcaacccgg caggcaaccg actttccggg tcattcgcgc
    95641 cgtggacata gatcagcatg tccacatcct gtagcgtggt aattaaggcg tgataccagc
    95701 ggggatggct aaaatattca cccggcgtat caatatcgcc cttatcatta aattccacgg
    95761 cctgtgtttt tctggcgagg gtataattcc cctgcaacac attaaataac gttgttttcc
    95821 ctgccccgac ggtacccaca aacgcaattc gtttcatgaa tgcctcgtat taacttttgg
    95881 tcatttcaca aagcgtataa tttaataagc gacctaaacc actgacggtt tgtgataacg
    95941 cttcttctac tgcccccacc gagccataaa tcaccagcgc gccgctaaaa cggtcgagaa
    96001 aaccgatatg cacatcggca gctttcagcg ctaaatcacc ggcgatcatc gccgtttcgc
    96061 caggagttag cgtcataatg ccaattgcgc ccgcatccgg aacgccgatt ttcttcgcca
    96121 gttcctcgcc gggatgcgcg atgagatgcg ccagcgtgac ctgtttgccg ggcacaaatt
    96181 cctgaatgat gcgttcttta tccatgagtc gccgcctgtg aaaaacttgc gtgtatcgtg
    96241 acaatttttc gtcagagtaa ataacaaaat tcggcaagcg cgtttaaaag gtgaggtaga
    96301 tcactaagaa tggagagata tttactgtta tgaataaact ttcatatgcg acgcatgaat
    96361 ataaataaaa taatctcgag atattttata tactgctttt tttattattc gcaaactcag
    96421 taataagctt tactaatata ttgcagccaa accgcttacc ctgaatattc agggtaagcg
    96481 tgagagttaa aaaagaatta cagcggttgg gtttgcgctt caaccacggc cagcgccacc
    96541 atgttgacga tacgacgcac cgatgcgatc ggcgttaaca cgtgaaccgg tttcgccacg
    96601 cccatcagca ccggaccgac agtcacacct tccgagctgg aaacgcgcag taagttgtaa
    96661 ctaatgcggg cagcttccat gttcggcatc accagaatat tggcggaacc tttcaaaggg
    96721 ctgtccggca tacggtcgtt gcgaatcgct tccaccagcg ctgcatcgcc gtgcatttca
    96781 ccatcaatca tcagttctgg tgcacgttcc ctgaccagtt ccagcgcctg acgcattttg
    96841 ctcgacgacg ggcagtcaga agaaccaaag ttggagtgcg acaacaaagc aacgcgcggt
    96901 tcaataccga aacgacggac agtttctgcc gccatcaagg tgatctccgc cagctcttct
    96961 gcgtctggtt cgtcattgac gtaggtatcg gcaataaagg tgttaccact cggcagcagc
    97021 agcgcgttca ttgcacctgc ggtgtgaacg ccatcgcgat aaccaaagac atttttcacc
    97081 acgctaaaat gttcatgata atcgcccacc gtaccgcaga tcattgcatc ggcttcccca
    97141 cgctgaacca tgatcgcgcc gatcactgtc gggttactga tcagcgcacg ctgcgcctgt
    97201 tcctgagtga caccgcgacg cttcatgatc tggaagtatt cggtccagta ctctttaaag
    97261 cgcggatcgg attcgttatt gacgatctca aaatcaacgc ccgctttgat ctgcaagccc
    97321 agtttctgaa tgcgcatttc gatcacgttc ggacgaccga taaggatcgg tttcgccagt
    97381 cccagcgtta ccagttcctg agtggcatgc agaacgcgcg cctcttcccc ttccggcaga
    97441 actacgcgct tcggcgcttt gcgagcctgg gagaaaatcg gcttcataaa caggttggtt
    97501 ttgtaaacga actcagtcag cttgtcgatg tagacgtcga aatcagcaat cggacgcgtc
    97561 gccacgcccg actccatcgc cgctttcgcg accgcaggag cgatcttaac gatcaagcgc
    97621 ggatcaaacg gttttggaat gatgtattcc ggaccaaagc tcagatcctg atcgccatac
    97681 gctgaagcca ccacttcgct ctgttccgca tgggccagtt ctgcaatcgc gcgtaccgcc
    97741 gccagtttca tctcttcgtt gatggcggtt gcgccaacgt ccagcgcgcc acggaagatg
    97801 aacgggaagc acaggacgtt gttcacctgg tttggatagt cagaacgacc ggtgcagatg
    97861 atggcatccg gacgcacttc tttcgccagc ggcggcagaa tttccggttc cgggttcgcc
    97921 agcgccagga tcattggcgc acgagccatt ttcttcacca tttcctgggt cagcactttc
    97981 gggccggaac agccgaggaa aatatccgcg ccttcaatca catcatcgag ggtacgtttg
    98041 ccgtcatcca ccaccgcata tgcggctttg gtttccgcca tgtttggctc acggccctga
    98101 tagataacgc cttttgaatc gcaaaccacg atgttatgtt tttgcagacc cagcgcgacc
    98161 agcaggttca tacaggcgat tgctgcggcg cccgcgccgg aaaccaccat ccgcacgtca
    98221 gagatgtttt tctccaccac gcgcaagccg ttgaggatgg cggcagtgct gataattgcc
    98281 gtgccgtgct gatcgtcgtg gaataccgga atattcatcc gctcgcgcag tttctgttca
    98341 atatagaaac attctggcgc tttaatgtct tcgaggttga tgccgccgaa ggtcggttcg
    98401 agcgcagcga cgacttcaat aaatttgtcc gggtcgagtt catcaacttc aatgtcaaat
    98461 acatcaatcc cggcgaattt cttaaacaga acgcccttgc cttccatcac cggtttgcct
    98521 gccagcgcgc caatgttgcc taaccccagc accgccgtac cgttagagat caccgccacc
    98581 aggttaccgc gcgcggtata tttgtaggct tttaacgggt ctttttcgat ttcaagacaa
    98641 ggtgcggcaa cgcctggtga gtaggccagc gccagatcgc gctgcgttgc cagaggcttg
    98701 gttggagaaa cctggatttt ccctggaact ggaaattcat ggaaatcaag tgcactttgt
    98761 tttaactggt catccatttg gttgttcctt tcacgtaacg ttcacaaata aagtgtgtgg
    98821 gcaacagccc ctgcccacaa cgtggcgcac attattacct tgccggagtc tacagacttt
    98881 gagcaagtcc aaactctcac cattaatata atgttttggt aataatccta taacactgat
    98941 gttacctgct taatccagca ataccatgcc tgtctgctat gcttttttga tgcgtttagc
    99001 gaaatttctc agaagtgtga attaacgcac tcatctaaca ctttactttt caaggagtat
    99061 ttcctatgaa cgagttagac ggcatcaaac agttcaccac tgtcgtggca gacagcggcg
    99121 atattgagtc cattcgccat tatcatcccc aggatgccac caccaatcct tcgctgttac
    99181 tcaaggctgc cggattatca caatatgagc atttaataga cgatgctatc gcctggggta
    99241 aaaaaaatgg caagacccag gaacaacagg tggtcgcagc gtgtgacaaa ctggcggtca
    99301 atttcggtgc tgaaatcctc aaaatcgtac ccggtcgcgt gtcaacagaa gttgatgcac
    99361 gcctctcttt tgataaagaa aagagtattg agaaggcgcg ccatctggtg gacttgtatc
    99421 agcaacaagg cgttgagaaa tcacgcattc tgatcaaact ggcttcgacc tgggaaggaa
    99481 ttcgcgcggc agaagagctg gaaaaagaag gtattaactg caacctgacg ctgctgtttt
    99541 cttttgcaca ggcacgggcc tgtgcagaag caggcgtttt tctgatttcg ccgtttgtcg
    99601 ggcgtattta tgactggtat caggcacgca agccgatgga cccgtatgtg gtggaagaag
    99661 atccaggcgt taaatcagta cgcaatatct acgactacta taagcaacac cactatgaaa
    99721 ccattgtgat gggcgcgagc ttccgtcgca ccgaacaaat cctcgcctta accggctgcg
    99781 atcgactgac tatcgcaccg aatttgctga aggagctgca ggaaaaagtt tcgccagtgg
    99841 tacgtaaatt aatcccacct tctcagacgt tcccacgccc agctcccatg agcgaagcgg
    99901 agttccgttg ggagcacaat caggatgcga tggcggtaga aaaactgtct gaaggcattc
    99961 gtctgttcgc cgttgatcaa cgcaaactgg aagatcttct tgccgccaaa ctataaacca
   100021 gccacggagt gttatatgtc ccgaaaagac cttgccaatg cgattcgcgc actcagtatg
   100081 gatgcggtac aaaaagccaa ttctggtcat cccggcgcgc cgatgggcat ggctgatatt
   100141 gcagaagtgc tgtggaacga ttttcttaaa cataacccta ccgacccaac ctggtatgat
   100201 cgcgaccgct ttattctttc caacggtcac gcgtcgatgc tgctctacag tttgctacat
   100261 ctgaccggtt acgacctgcc gctggaagaa ctgaagaact tccgtcagtt gcattcgaaa
   100321 acccctggcc acccggaaat cggctatacg cccggtgttg aaaccaccac cggtccactt
   100381 ggacaaggtt tggcgaacgc cgtcggactg gcgatagcag agcgtacact ggcggcgcag
   100441 tttaaccagc cggatcatga aattgtcgat cacttcacct atgtgtttat gggcgacggc
   100501 tgcctgatgg aaggtatttc ccacgaagtc tgttcgctgg cgggcacgct gggactgggc
   100561 aagctgattg gtttttacga tcacaacggt atttcgattg atggagaaac cgaaggctgg
   100621 tttaccgacg atacggcaaa acgttttgaa gcctatcact ggcatgtgat ccatgaaatc
   100681 gacggtcacg atccgcaggc ggtgaaggaa gcgatccttg aagcgcaaag cgtgaaagat
   100741 aagccgtcgc tgattatctg ccgtacggtg attggctttg gttcgccgaa taaagcaggt
   100801 aaggaagagg cgcacggcgc accgctgggg gaagaagaag tggcgctggc acggcaaaaa
   100861 ctgggctggc accatccgcc atttgagatc cctaaagata tttatcacgc ctgggatgcc
   100921 cgcgaaaaag gcgaaaaagc gcagcagagc tggaatgaga agtttgccgc ctataaaaag
   100981 gctcatccgc aactggcaga agagtttacc cgtcggatga gcggtggttt accgaaggac
   101041 tgggagaaaa cgactcagaa atatatcaat gagttacagg caaatccggc gaaaatcgcc
   101101 acccgtaagg cttcgcaaaa tacgcttaac gcttacggac cgatgctacc ggagctgctc
   101161 ggcggttcgg cggatctggc tcccagcaac ctgaccatct ggaaaggctc tgtgtcgctg
   101221 aaggaagatc cggcgggaaa ttatattcac tacggggtgc gtgaatttgg catgaccgct
   101281 atcgccaacg gcattgcgca ccacggcggc tttgtgccgt ataccgcgac attcctgatg
   101341 tttgttgaat acgcccgtaa tgccgcgcgg atggcggcac tgatgaaagc acggcagatt
   101401 atggtttata cccacgactc aattggcctg ggcgaagatg gtccgacgca ccaggctgtt
   101461 gagcaactgg ccagcctgcg cttaacgcca aatttcagca cctggcgacc gtgcgatcag
   101521 gtggaagcgg cggtgggctg gaagctggcg gttgagcgcc acaacggacc gacggcactg
   101581 attctctcca ggcagaatct ggcccaggtg gaacgtacgc cggatcaggt taaagagatt
   101641 gctcgtggcg gttatgtgct gaaagacagc ggcggtaagc cagatattat tttgattgcc
   101701 accggttcag agatggaaat caccctgcaa gcggcagaga aattagcggg agaaggtcgc
   101761 aatgttcgcg tagtttccct gccctcgacc gatattttcg acgcccagga tgaggaatat
   101821 agggagtcgg tgttgccttc taacgttgcg gctcgcgtgg cggtggaagc aggtattgcc
   101881 gattactggt acaagtatgt tggtctgaaa ggggcaattg tcgggatgac gggttacggg
   101941 gaatctgctc cggcggataa gctgttcccg ttctttggct ttaccgccga gaatattgtg
   102001 gcaaaagcgc ataaggtgct gggagtgaaa ggtgcctgat ggtgatcgcc ggatgctgat
   102061 tgccggatgc gacgctgacg cgtcttatcc ggcctacagc gtcttatccg gcctacatgt
   102121 cccgccattt tgtttaacgg gtgatccaca acgtgggcca ggcgtctggc ccatgccagt
   102181 tatcgcaggt gggttctgcg gcgtaacgca ccaggcgaaa acgctgaccg tcaaagcgcc
   102241 agcgcgcctg aatgccacaa tcgcttaatc cgcgcccttt cgctaaggtc accagttcac
   102301 gcgatttctc atcaaatgtt gcgttcatca gttccagttc atttgtctcc tgcccgttgt
   102361 tgaacggcaa acgcaaccga accggacgcg aggctagtgg ctttttacgc gacacaatcc
   102421 atgccaaatc aatggtgtta taggcccctg cctcacagct aatcatcatc agcgctttat
   102481 catcagtcag cgcagtgaca ttcacctcac gacgtaacgg atcaagcgag cagcgcagac
   102541 cattcatccg ccagtttcca taatccagca aatcgttacg ttcttcgagt gagagtggcg
   102601 tcggcgttgg gttaaccacc gcgacctctt tcaacgcagg cgctggcggt acgctgagcg
   102661 gcggttcgtc ccctttcttt atccacgcgg tttcactgcc aacgcgcttt tgctgagcat
   102721 caataaacaa caacgccgct tttaagccac tcagagaaat ggtctgattg ccgtcgcgta
   102781 aggtgatcgc cttcccttcc tgaatcatct gcaaaaacgc ggtgatggtt gccgtatcat
   102841 cggttactaa taaccatggt gaaatccgcc acttgtcacc acttaacgcc aacggctcac
   102901 catctaacag cagccgtggc gctatctccc cttcagacgc atccggcgat ttcaacccgc
   102961 cgcgctcaat acgtaaaacg gcatcggtat gcgccccggc gctgcggctc agggtcatca
   103021 ccagtccatt atgatcgccc gtattgcgcg ccacgcagaa gttttggtta ttacaggtga
   103081 cctgccagtc ggaaaacgcc cgttgcgctg gtgccgccca caccaaagac gtcggcaaca
   103141 aagcgaaaaa aaagagaagg agattgcgat agcgcatgga cggtacgacc ccagaagttc
   103201 aaacaaacag cacaagtcgt atcttcgttg cgcaggtagc gtggctcaat cggatttgtc
   103261 ggattgagcc aataataaac aacgaaatat tattgttatt tttcagtcca ttaaatgtga
   103321 cgtttgcaaa tagttaagca ataacaccgt cttaccgtca cgtatctcgc cggttttgat
   103381 catctccagc gcctggctga acggcagctc gagcacttca atatcttcat cttcgacacc
   103441 gccaccggcg ttagcgcgct gattgtcact gtattcggcg ataaaaaagt ggattagctc
   103501 agtcacaccg cctggcgaca tatacagttc aaataatttg cgcacttcgc ccacttcata
   103561 gcccgtctct tcaatcgctt ctttacgaat gcacacttcc ggttcgtcgt tatccagcag
   103621 cccggcgcag gtttcaatca gctgcccgct ttcattgcca ttaacccagg tagcgacacg
   103681 gaactgacga atcagaacca cggtcttttt cttcgcgttg tacaggagga tcgtcgcacc
   103741 attaccgcga tcgtaaactt cacgtttatg gcggataact tcgccatctt tgcgcgtgag
   103801 atcgtaagta atgttgtgca gggtgaaata gttatcggag agaattttgt ctttaatgag
   103861 ggtgatttgt tgcgtcatac cgactccaca gcgcgaaatg aacaattata ttacgctgtg
   103921 aagtcgggtt tgtctgcgca ggctatcagt gagatttgac tgattttacc cccagccagt
   103981 caataatccc ctgtgccgca tgacgtcctt ctgccattgc ggtaaccacc agatccgcac
   104041 cacgcacggc gtcaccacca gcgaagattt tcggattggt ggtctggtaa cggtactggc
   104101 tttccacatc cgcgatgatg cggccccatt tgtctaccgt tacaccgtgc gactccagcc
   104161 acggcatccc gtgcggattg aagccaaacg ccataatcac cgcgtcggct ggcatgacaa
   104221 attcactgcc ttccaccggc actggacgcc tacgcccctg ggcatccggc tcgccaagac
   104281 gcgtacgcag gaaacgaatc ccgcaaacgt gaccttgttc attcagctca agcgccaccg
   104341 gctggacgtt aaattcgaag ttggccccct cttcgcgggc gttcttcacc tctttcttcg
   104401 agcctggcat gttagcttca tcacgacgat aagcgcaggt gacgttactc gcgccgtgac
   104461 gcagcgcagt acgcacacag tccatcgcgg tgtcgccgcc gcccagtacc acgacgttaa
   104521 gtccggcggt attgataaac ggctcttccg gtagctcttc gagccccatc acctgtttag
   104581 tgttggcgat gaggaacggc agcgcgtcat aaacgcccgg cgcatcttca ttgggtaaac
   104641 ccgctttcat ggaacggtaa gtgcctacgc caacgaagac cgcgtcgtat tgttccaaaa
   104701 gcgaatccaa agagacatct ttacccactt cacaattgag ttcgaagtga atccccatcg
   104761 cgctgaagat ttcccgacgg cgtgccagca gggatttatc cagtttgaaa gaaggaatgc
   104821 cgaaagtgag caagccaccg atttctggat ggcgatcgta caccgtcacc gccacgccat
   104881 tgcgggtcag aacatccgca caggccagcc ctgccggacc tgcaccgata atcgccaccc
   104941 gcttgtccac tttggtgaca tggcttaagt caggacgcca acctttcgcc aacgcctgat
   105001 ctgaaatgta gcgttcaatg ttgccgatag ttaccgcacc gtgctcatcg cgaatagtac
   105061 aggcaccttc acacaaacgg tcttgcggac aaacgcgtcc ggtaatttcc ggcagggtgt
   105121 tggtcaggtg agaaagctcg acggcggcgt cgatgtttcc ggctttcacc agttcaatcc
   105181 actgcggtat atggttatgc agcgggcagg tccattcaca aacgctgtgc tcgccgcatt
   105241 taaggcagcg cgaggcttcc cgttgtgcct gatcggcacg gaatggcaga taaatttcat
   105301 caaaaccggt tttgcgcgct tcaatcgcca gtttatccgg ctcgccacgc gcgggcgttg
   105361 cctgcatttg ctcgacttta ctcattaccg gcatttcttg cgccgcggta ctggcatgcc
   105421 acggttgatg ttcctgacgt gcggtacgca agcggcggga tttcgccatg cctgagagtg
   105481 cggcgtcagt gaccagctgc agcgcgtccg ccgggcaatt ctcgacacag gcaggaccgt
   105541 tttcgcgccc tgcacaaagg tcgcatttat gcgccgtggc tttaactttg cccgctgcga
   105601 cgggtgtcag gacgatttgc atcgtaccaa acggacaggc caccacgcag gatttacagc
   105661 caatacactt ttgctgattg acctgaatgc tgtcatcaac gtggctgatt gcgccattag
   105721 ggcagctacg ggcgcagggc gcatcttcac aatggtgaca ggtcactgca ctacgttgct
   105781 gctgatgttt gataaccgta attcggggat gaaaatgatg ttggctcagg acatgttgct
   105841 catcattgtg agccatgaca caagcgattt cacaagcatg acaacccaga cattgctgac
   105901 tgttggccat aataaaacga ttcataacga ccttcttttt tggttgtaaa aaccttattc
   105961 tttatatgag tgttgttatt acccgactta caggggaatc ggcaatgttc atgtgcccag
   106021 aataagtaac tatttcgcta taaactgtgg cagatcaaat aatcccatca ctgactaaat
   106081 tgcgtttcag cgaactggaa cattaatgat tttttgtgga gaagacgcgt gtgattgtta
   106141 aacgacccgt ctcggccagt ctggcccggg ccttttttta cattgtgctg ctgtcgattc
   106201 tttccacggg tatcgctctg ctaactctgg cgagcagttt gcgcgacgct gaggctatca
   106261 atattgccgg atcgctgcgt atgcagagtt accgcctggg ctacgacttg caaagtggca
   106321 gtccacaact caatgcacat cgccagttat ttcagcaggc actgcattca ccggtattaa
   106381 ccaacctcaa cgtctggtat gtgccagaag cagtaaaaac tcgctatgcg catctgaatg
   106441 ccaactggct ggagatgaat aatcggctca gcaagggcga tttgccgtgg tatcaggcca
   106501 atatcaataa ttatgttaat cagatagacc tgttcgtgct ggctttacag cactacgctg
   106561 aacgcaaaat gctgctggtg gtggcgattt ccctggctgg cggcatcggt attttcacgc
   106621 tggtcttttt tactttgcgc cgcatacgcc atcaggtggt tgccccgctg aatcagctgg
   106681 ttaccgccag tcagcgtatt gaacacgggc agttcgactc gccaccgctg gataccagcc
   106741 tgccgaatga gcttggtctg cttgcaaaaa cctttaacca gatgtcgagc gagctgcata
   106801 aattgtacct ttcgctggag gcgtcagtag aagaaaagac ccgcgatctc cacgaggcca
   106861 agcgtcgtct ggaggtgttg tatcaatgtt cgcaggcgct gaacaccagc cagattgatg
   106921 tgcattgttt ccgccatatt ttgcagattg ttcgcgataa tgaagcggct gaatatctgg
   106981 agttaaatgt cggtgaaaac tggcggatta gcgaagggca accaaacccg gaattgccga
   107041 tgcagatttt accggtgaca atgcaagaga cggtttacgg cgaactgcac tggcaaaata
   107101 gtcacgtttc atcatcagaa ccgctgctta acagcgtttc gtcgatgctg ggacgcggtt
   107161 tgtactttaa tcaggcgcag aagcattttc agcagttatt gttgatggaa gaacgtgcaa
   107221 ccatcgcccg cgaattgcac gactcgctgg ctcaggtact ttcttactta cgtatccagt
   107281 tgacgttact gaagcgttcg ataccggaag ataacgccac cgcacaaagt atcatggccg
   107341 atttttccca ggcgttgaat gatgcttatc ggcagttacg cgagctgttg actacttttc
   107401 gcctgacgct gcagcaggcg gatctcccct ccgcgttgag ggaaatgctg gatacgttac
   107461 aaaatcaaac cagcgccaaa ctgaccctcg actgccgtct gccaaccctg gcactggatg
   107521 cgcaaatgca ggtgcatttg ttgcaaatta ttcgcgaagc ggtgctgaat gcgatgaagc
   107581 acgccaacgc cagcgaaatc gccgtcagtt gcgtcaccgc gccggacggc aatcacacgg
   107641 tttatatccg tgataacggg attggtatcg gtgaaccgaa agaacccgaa ggtcattatg
   107701 gtctgaatat catgcgcgaa cgcgcggaac ggctaggtgg gacgctgact ttttcacaac
   107761 cttccggcgg cggtacgtta gtgagtatta gctttcgctc tgcggagggt gaggaaagtc
   107821 agttaatgta atgcctccta ctgaccaaag aatgcttgca cttaaggttc agtataaaag
   107881 ggcatgataa tttacattaa ctcctttttt tctccacgat tggctcgtac cttgccgcta
   107941 cagtgaagca agtcaagcct acaacgatac gcagaaacac gaggtcctct tttaatggcg
   108001 aatttcttta ttgatcgccc catttttgcc tgggtgctgg caatcctgtt gtgtctgaca
   108061 ggtaccctgg cgattttttc attgcccgtt gaacaatacc ccgatctcgc gccaccgaat
   108121 gtgcgagtga ccgctaacta tcccggcgca tcggcccaga cgctggaaaa caccgtgacc
   108181 caggttatcg agcaaaatat gactggcctc gataatctca tgtatatgtc atctcagagc
   108241 agtggcaccg gtcaggcatc tgtcacttta agttttaaag caggcaccga tccggacgaa
   108301 gccgtgcagc aagtacaaaa ccagctgcaa tcagccatgc gaaagttacc gcaggcggtg
   108361 caaaatcagg gcgtgacggt gcgtaaaacc ggcgatacca acattctgac cattgccttc
   108421 gtctctaccg atggttcgat ggataaacag gatattgctg attatgttgc cagtaatatt
   108481 caggacccgt taagccgcgt gaatggcgtc ggggatatcg atgcctatgg ttcgcaatat
   108541 tccatgcgta tctggctgga cccggcgaaa ctcaacagtt tccagatgac ggctaaagat
   108601 gtcactgatg ccattgagtc acagaacgcg cagattgcgg ttgggcaact tggtggtaca
   108661 ccttccgtcg ataagcaggc gctcaacgcc accattaacg cccagtcact gctgcaaaca
   108721 ccagaacagt tccgcgatat caccttgcgg gtgaatcaag acggctcaga ggtaaggctg
   108781 ggcgatgtcg ccaccgtcga aatgggggcg gagaaatacg attatcttag ccgcttcaat
   108841 ggtaagccag cctccgggct gggggtaaaa ctggcctccg gcgctaacga aatggcgaca
   108901 gcggagctgg tgctcaatcg tctcgacgag ctggcgcagt atttcccgca tggactggaa
   108961 tacaaggtgg cgtatgaaac cacctcgttt gttaaagcct ccattgaaga cgtggtgaaa
   109021 acgctgctgg aagctatcgc tctggttttc ctcgttatgt atctgttcct gcaaaacttc
   109081 cgcgccacgc tgatacccac tatcgccgtg ccggtggtgt tgatgggaac cttctccgta
   109141 ctttacgcct tcggttacag cgtcaacacc ttaaccatgt tcgccatggt gctggcgatc
   109201 ggtctgctgg tggatgatgc catcgtggtg gtggaaaacg tcgaacgtat tatgagtgag
   109261 gaaggactca ctcctcgcga agccacacgt aaatcgatgg ggcagatcca gggggcactg
   109321 gtcgggattg cgatggtgct ttcggcggta tttgtaccaa tggccttctt cggcggcacc
   109381 accggtgcca tctatcgcca gttctctatt accattgttg cggcgatggt gctgtcagta
   109441 ctggtagcga tgatcctcac tccggctctg tgtgccacgc tacttaagcc actgaaaaaa
   109501 ggtgagcatc acgggcaaaa aggctttttt gcctggttta accagatgtt taaccgcaac
   109561 gccgaacgct acgaaaaagg ggtggcgaaa attctccacc gtagcctgcg ctggattgtg
   109621 atttatgtcc tgctgcttgg cggcatggtg ttcctgttcc tgcgtttgcc gacgtcgttc
   109681 ttaccgctgg aagaccgtgg catgtttact acctcggtac aattgcccag cggttcaacc
   109741 caacaacaga ccctgaaagt cgttgagcaa atcgagaaat attacttcac ccatgaaaaa
   109801 gacaacatca tgtcggtgtt tgccactgtt ggttctggcc ctgggggtaa cgggcaaaac
   109861 gtggcgcgaa tgtttatccg cctgaaagac tggagcgaac gcgacagtaa gaccggcacc
   109921 tcatttgcca ttatcgagcg tgcaacaaag gcgtttaacc aaattaaaga agctcgcgtt
   109981 atcgccagca gcccgccagc aattagcggt cttggtagtt ctgcaggttt tgatatggag
   110041 ttgcaggacc acgctggagc aggtcacgat gcgctgatgg cagcacgtaa ccagttgctg
   110101 gcgctggcgg cggaaaaccc ggagctaacc cgtgtgcgcc ataacggcct cgatgacagt
   110161 ccgcagttgc agattgatat cgaccagcgt aaagctcagg cgctgggcgt tgctatcgac
   110221 gatattaacg acacactgca aaccgcctgg ggttcgagct atgtgaatga ctttatggat
   110281 cgcggtcgcg tgaagaaagt ctatgtgcag gcagctgcgc cgtatcgcat gctgccagat
   110341 gacatcaatc tctggtatgt ccgaaataaa gatggcggca tggtgccctt ctctgctttc
   110401 gcgacctcac gctgggagac aggttcgccg cgtctggaac gctataacgg ttattctgcg
   110461 gttgagattg ttggggaagc cgcaccgggg gtcagtaccg gtacggcgat ggatattatg
   110521 gaatcgttag tgaagcagtt gccaaacggc tttggtctgg agtggacggc gatgtcgtat
   110581 caggagcggc tttccggcgc gcaggctccg gcgctgtacg ctatttcctt gctggtggta
   110641 ttcctgtgtc tggcggcgtt gtatgaaagc tggtcggtac cgttctcggt aatgctggtc
   110701 gtgccgctgg gggtaatcgg cgcgctgctg gcaacctgga tgcgcgggct ggaaaacgac
   110761 gtttacttcc aggtgggcct gttaacggtc attggtttat cggcgaaaaa cgccatcctg
   110821 atcgtcgagt ttgctaacga gatgaaccaa aaaggccacg acctgtttga agcgacgctc
   110881 cacgcctgcc gtcagcgttt acgcccgatt ctgatgacct cgctggcatt tatcttcggc
   110941 gtattgccaa tggcaaccag cacgggtgca ggttccggtg gtcagcatgc ggtgggtact
   111001 ggcgtaatgg gcgggatgat ttcagccact attctggcta tttacttcgt gccgctgttc
   111061 tttgtgctgg tgcgccgccg cttcccgctg aagccgcgcc cggaataagc aataaaaaag
   111121 gcgacatgct aatgtgtcgc ctttttcaac tttccgatta agaacctgct cagcgggttc
   111181 ttgctgtttg tactttgtct caggaattac ttacgaagca taacttcgat aaagtctttc
   111241 cagttcccca gttcacgttc aatcataaca acctctctta taattatggg tattctacgg
   111301 aaacaatata ccgtggtgaa gctaatttac tcgattgctg cgatgactac ctccggggga
   111361 caaaccttat gtaaatacta tggtcctgcg gtgataattt gtatgtgata cacagcaaca
   111421 tttcgagata ttcatacggc atctaatact gatttaattc tggttaaaat acagacagat
   111481 aacaagat
//
LOCUS       DAZFKF010000003       104110 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFKF010000003 DAZFKF010000000
VERSION     DAZFKF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 104110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 104110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..104110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.003"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..289
                     /locus_tag="ACHKA1_000246"
     CDS             <1..289
                     /locus_tag="ACHKA1_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5677953.1"
                     /translation="IDITDKKLAPLINDSFSYTRDFFAYSKQEKNIFTFDNSKFVDPK
                     EKEGLMVQHSNGQLVITGKYCPEGVQTAFTQEQYDKLIRYINIFFTFPKLE"
     gene            complement(513..1589)
                     /gene="glpQ"
                     /locus_tag="ACHKA1_000247"
     CDS             complement(513..1589)
                     /gene="glpQ"
                     /locus_tag="ACHKA1_000247"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO5677954.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1594..2952)
                     /gene="glpT"
                     /locus_tag="ACHKA1_000248"
     CDS             complement(1594..2952)
                     /gene="glpT"
                     /locus_tag="ACHKA1_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO5677955.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            3225..4853
                     /gene="glpA"
                     /locus_tag="ACHKA1_000249"
     CDS             3225..4853
                     /gene="glpA"
                     /locus_tag="ACHKA1_000249"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO5677956.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVHVRNHLTGETQSLHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAQTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4843..6102
                     /gene="glpB"
                     /locus_tag="ACHKA1_000250"
     CDS             4843..6102
                     /gene="glpB"
                     /locus_tag="ACHKA1_000250"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO5677957.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELGLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            6099..7289
                     /gene="glpC"
                     /locus_tag="ACHKA1_000251"
     CDS             6099..7289
                     /gene="glpC"
                     /locus_tag="ACHKA1_000251"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO5677958.1"
                     /translation="MNDTCFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7482..8453
                     /gene="rpnE"
                     /locus_tag="ACHKA1_000252"
     CDS             7482..8453
                     /gene="rpnE"
                     /locus_tag="ACHKA1_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO5677959.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSLLQKERLMTIAERLRQEGHQIGWQEGKLEGLQQGKLEGLQ
                     KGKLEGLQEGMHEQAIKIALRMLEQGFEREIVLAATQLTDADIPNCH"
     gene            8454..8687
                     /locus_tag="ACHKA1_000253"
                     /pseudo
     CDS             8454..8687
                     /locus_tag="ACHKA1_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8728..9531)
                     /gene="yfaU"
                     /locus_tag="ACHKA1_000254"
     CDS             complement(8728..9531)
                     /gene="yfaU"
                     /locus_tag="ACHKA1_000254"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFO5677960.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVDGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(9549..10838)
                     /gene="yfaV"
                     /locus_tag="ACHKA1_000255"
     CDS             complement(9549..10838)
                     /gene="yfaV"
                     /locus_tag="ACHKA1_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5677961.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(10895..12100)
                     /gene="rhmD"
                     /locus_tag="ACHKA1_000256"
     CDS             complement(10895..12100)
                     /gene="rhmD"
                     /locus_tag="ACHKA1_000256"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFO5677962.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            complement(12115..12897)
                     /gene="yfaX"
                     /locus_tag="ACHKA1_000257"
     CDS             complement(12115..12897)
                     /gene="yfaX"
                     /locus_tag="ACHKA1_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO5677963.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(13117..14319)
                     /gene="yfaY"
                     /locus_tag="ACHKA1_000258"
     CDS             complement(13117..14319)
                     /gene="yfaY"
                     /locus_tag="ACHKA1_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO5677964.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(14419..14961)
                     /gene="yfaZ"
                     /locus_tag="ACHKA1_000259"
     CDS             complement(14419..14961)
                     /gene="yfaZ"
                     /locus_tag="ACHKA1_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO5677965.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQVGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            15240..15665
                     /gene="nudI"
                     /locus_tag="ACHKA1_000260"
     CDS             15240..15665
                     /gene="nudI"
                     /locus_tag="ACHKA1_000260"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO5677966.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEIKPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(15704..16306)
                     /gene="ais"
                     /locus_tag="ACHKA1_000261"
     CDS             complement(15704..16306)
                     /gene="ais"
                     /locus_tag="ACHKA1_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO5677967.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            16614..17753
                     /gene="arnB"
                     /locus_tag="ACHKA1_000262"
     CDS             16614..17753
                     /gene="arnB"
                     /locus_tag="ACHKA1_000262"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO5677968.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADINAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWSHVHAWHLFIIRVDEQRCGISRNALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            17757..18725
                     /gene="arnC"
                     /locus_tag="ACHKA1_000263"
     CDS             17757..18725
                     /gene="arnC"
                     /locus_tag="ACHKA1_000263"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO5677969.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHILVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            18725..20707
                     /gene="arnA"
                     /locus_tag="ACHKA1_000264"
     CDS             18725..20707
                     /gene="arnA"
                     /locus_tag="ACHKA1_000264"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO5677970.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPVCTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            20704..21594
                     /gene="arnD"
                     /locus_tag="ACHKA1_000265"
     CDS             20704..21594
                     /gene="arnD"
                     /locus_tag="ACHKA1_000265"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO5677971.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            21594..23246
                     /gene="arnT"
                     /locus_tag="ACHKA1_000266"
     CDS             21594..23246
                     /gene="arnT"
                     /locus_tag="ACHKA1_000266"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO5677972.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            23243..23578
                     /gene="arnE"
                     /locus_tag="ACHKA1_000267"
     CDS             23243..23578
                     /gene="arnE"
                     /locus_tag="ACHKA1_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO5677973.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            23578..23964
                     /gene="arnF"
                     /locus_tag="ACHKA1_000268"
     CDS             23578..23964
                     /gene="arnF"
                     /locus_tag="ACHKA1_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO5677974.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(23958..24224)
                     /gene="pmrD"
                     /locus_tag="ACHKA1_000269"
     CDS             complement(23958..24224)
                     /gene="pmrD"
                     /locus_tag="ACHKA1_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO5677975.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKVAGKTQ"
     gene            complement(24334..25689)
                     /gene="menE"
                     /locus_tag="ACHKA1_000270"
     CDS             complement(24334..25689)
                     /gene="menE"
                     /locus_tag="ACHKA1_000270"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO5677976.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(25686..26648)
                     /gene="menC"
                     /locus_tag="ACHKA1_000271"
     CDS             complement(25686..26648)
                     /gene="menC"
                     /locus_tag="ACHKA1_000271"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO5677977.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRG
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(26648..27505)
                     /gene="menB"
                     /locus_tag="ACHKA1_000272"
     CDS             complement(26648..27505)
                     /gene="menB"
                     /locus_tag="ACHKA1_000272"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO5677978.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(27520..28278)
                     /gene="menH"
                     /locus_tag="ACHKA1_000273"
     CDS             complement(27520..28278)
                     /gene="menH"
                     /locus_tag="ACHKA1_000273"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO5677979.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEVFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQRLAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRSEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(28275..29945)
                     /gene="menD"
                     /locus_tag="ACHKA1_000274"
     CDS             complement(28275..29945)
                     /gene="menD"
                     /locus_tag="ACHKA1_000274"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO5677980.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRVCDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(30034..31329)
                     /gene="menF"
                     /locus_tag="ACHKA1_000275"
     CDS             complement(30034..31329)
                     /gene="menF"
                     /locus_tag="ACHKA1_000275"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO5677981.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVFARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(31408..31713)
                     /gene="elaB"
                     /locus_tag="ACHKA1_000276"
     CDS             complement(31408..31713)
                     /gene="elaB"
                     /locus_tag="ACHKA1_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO5677982.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(31768..32229)
                     /gene="elaA"
                     /locus_tag="ACHKA1_000277"
     CDS             complement(31768..32229)
                     /gene="elaA"
                     /locus_tag="ACHKA1_000277"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5677983.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            32294..33211
                     /gene="rbn"
                     /locus_tag="ACHKA1_000278"
     CDS             32294..33211
                     /gene="rbn"
                     /locus_tag="ACHKA1_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO5677984.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            33399..34622
                     /locus_tag="ACHKA1_000279"
     CDS             33399..34622
                     /locus_tag="ACHKA1_000279"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFO5677985.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNDTLSIIKLIKNACLKLYNLAHTATK
                     HPLNSHDSDNLLFKKLFSPSKLMTIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFW
                     DKNRQLTTDEISCYLKKIAANAKNTQVNYPTDFYLPNSNSTYLEVALNDNIKSDPSWP
                     KEVQLFPINTGGHWILVSLQKIVNEKNNTQQIKCIIFNSLRSLGHEKENSLKRIINSF
                     NSFNCDPTRETPNNKNITDHLTEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQMES
                     NSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDANINHYYRNLEI
                     KHSQPKNRASSKRVS"
     gene            complement(34693..36420)
                     /gene="yfbK"
                     /locus_tag="ACHKA1_000280"
     CDS             complement(34693..36420)
                     /gene="yfbK"
                     /locus_tag="ACHKA1_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HFO5677986.1"
                     /translation="MRNKNIIMLLMSSLILSGCGPQPENKESQQQQPSTPTEQQVLAA
                     QQAAIKEAEQSAAAAKALAQQEVQQYSDKQALQGRLQEAPTFARAAKAKATHIANPGT
                     ARYQQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNQGLLPPPDAVRVEEIVNYF
                     PSDWDIKDKQSIPASKPIPFAMRYELAPAPWNEQRTLLKVDILAKDRKSEELPASNLV
                     FLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSHKAE
                     INAAIDSLDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNVGIDDPKSIE
                     LMLKKQRESGVTLSTFGVGDDNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSEMRQ
                     TLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKHITLLF
                     ELTLNGQKASIDKLRYAPDNKLAKSDKTKELAWLKIRWKYPQGKESQLVEFPLGPTIN
                     APSEDMRFRAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGVDPQGYRAEFIRLIEL
                     ADGVTDISQ"
     gene            36608..37528
                     /gene="yfbL"
                     /locus_tag="ACHKA1_000281"
     CDS             36608..37528
                     /gene="yfbL"
                     /locus_tag="ACHKA1_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO5677987.1"
                     /translation="MIIFYQPWVNALPSTPRHASPEQLEKTVRYLTQTVHPRSADNID
                     NLNRSAEYIKEVFVSSGARVTSQDVPITGGPYKNIVADYGPADGPLIIIGAHYDSASS
                     YENDQLTYTPGADDNASGVAGLLELARLLHQQVPKTGVQLVAYASEEPHFFRSDEMGS
                     AVHAASLEHPVKLMIALEMIGYYDSAPGSQNYPYPAMSWLYPDRGDFIAVVGRMQDIN
                     AVRQVKAALLSSQDLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAIMITDTAFYRNKQY
                     HLPGDTADRLNYQKMAQVVNGVITLLYNSK"
     gene            37631..38134
                     /gene="yfbM"
                     /locus_tag="ACHKA1_000282"
     CDS             37631..38134
                     /gene="yfbM"
                     /locus_tag="ACHKA1_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO5677988.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRNELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(38201..39658)
                     /gene="nuoN"
                     /locus_tag="ACHKA1_000283"
     CDS             complement(38201..39658)
                     /gene="nuoN"
                     /locus_tag="ACHKA1_000283"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO5677989.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(39665..41194)
                     /gene="nuoM"
                     /locus_tag="ACHKA1_000284"
     CDS             complement(39665..41194)
                     /gene="nuoM"
                     /locus_tag="ACHKA1_000284"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO5677990.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(41358..43199)
                     /gene="nuoL"
                     /locus_tag="ACHKA1_000285"
     CDS             complement(41358..43199)
                     /gene="nuoL"
                     /locus_tag="ACHKA1_000285"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO5677991.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(43196..43498)
                     /gene="nuoK"
                     /locus_tag="ACHKA1_000286"
     CDS             complement(43196..43498)
                     /gene="nuoK"
                     /locus_tag="ACHKA1_000286"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO5677992.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(43495..44049)
                     /gene="nuoJ"
                     /locus_tag="ACHKA1_000287"
     CDS             complement(43495..44049)
                     /gene="nuoJ"
                     /locus_tag="ACHKA1_000287"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO5677993.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(44061..44603)
                     /gene="nuoI"
                     /locus_tag="ACHKA1_000288"
     CDS             complement(44061..44603)
                     /gene="nuoI"
                     /locus_tag="ACHKA1_000288"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO5677994.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(44618..45595)
                     /gene="nuoH"
                     /locus_tag="ACHKA1_000289"
     CDS             complement(44618..45595)
                     /gene="nuoH"
                     /locus_tag="ACHKA1_000289"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO5677995.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(45592..48318)
                     /gene="nuoG"
                     /locus_tag="ACHKA1_000290"
     CDS             complement(45592..48318)
                     /gene="nuoG"
                     /locus_tag="ACHKA1_000290"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO5677996.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(48371..49708)
                     /gene="nuoF"
                     /locus_tag="ACHKA1_000291"
     CDS             complement(48371..49708)
                     /gene="nuoF"
                     /locus_tag="ACHKA1_000291"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO5677997.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(49705..50205)
                     /gene="nuoE"
                     /locus_tag="ACHKA1_000292"
     CDS             complement(49705..50205)
                     /gene="nuoE"
                     /locus_tag="ACHKA1_000292"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO5677998.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(50208..52010)
                     /gene="nuoC"
                     /locus_tag="ACHKA1_000293"
     CDS             complement(50208..52010)
                     /gene="nuoC"
                     /locus_tag="ACHKA1_000293"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO5677999.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(52104..52766)
                     /gene="nuoB"
                     /locus_tag="ACHKA1_000294"
     CDS             complement(52104..52766)
                     /gene="nuoB"
                     /locus_tag="ACHKA1_000294"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO5678000.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(52782..53225)
                     /gene="nuoA"
                     /locus_tag="ACHKA1_000295"
     CDS             complement(52782..53225)
                     /gene="nuoA"
                     /locus_tag="ACHKA1_000295"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO5678001.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(53856..54794)
                     /gene="lrhA"
                     /locus_tag="ACHKA1_000296"
     CDS             complement(53856..54794)
                     /gene="lrhA"
                     /locus_tag="ACHKA1_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO5678002.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
     gene            55714..56931
                     /gene="alaA"
                     /locus_tag="ACHKA1_000297"
     CDS             55714..56931
                     /gene="alaA"
                     /locus_tag="ACHKA1_000297"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO5678003.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            57015..57614
                     /gene="yfbR"
                     /locus_tag="ACHKA1_000298"
     CDS             57015..57614
                     /gene="yfbR"
                     /locus_tag="ACHKA1_000298"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO5678004.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(57673..59505)
                     /gene="yfbS"
                     /locus_tag="ACHKA1_000299"
     CDS             complement(57673..59505)
                     /gene="yfbS"
                     /locus_tag="ACHKA1_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO5678005.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(59592..60242)
                     /gene="hxpA"
                     /locus_tag="ACHKA1_000300"
     CDS             complement(59592..60242)
                     /gene="hxpA"
                     /locus_tag="ACHKA1_000300"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO5678006.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLDQITVTKQPNGDVIIQ"
     gene            complement(60253..60747)
                     /gene="yfbU"
                     /locus_tag="ACHKA1_000301"
     CDS             complement(60253..60747)
                     /gene="yfbU"
                     /locus_tag="ACHKA1_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO5678007.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(60830..61285)
                     /gene="yfbV"
                     /locus_tag="ACHKA1_000302"
     CDS             complement(60830..61285)
                     /gene="yfbV"
                     /locus_tag="ACHKA1_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO5678008.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQQDKTFLDDL"
     gene            61623..62825
                     /gene="ackA"
                     /locus_tag="ACHKA1_000303"
     CDS             61623..62825
                     /gene="ackA"
                     /locus_tag="ACHKA1_000303"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO5678009.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKDSGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            62900..65044
                     /gene="pta"
                     /locus_tag="ACHKA1_000304"
     CDS             62900..65044
                     /gene="pta"
                     /locus_tag="ACHKA1_000304"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO5678010.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            65234..66754
                     /gene="yfcC"
                     /locus_tag="ACHKA1_000305"
     CDS             65234..66754
                     /gene="yfcC"
                     /locus_tag="ACHKA1_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO5678011.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVIFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(66787..67329)
                     /gene="yfcD"
                     /locus_tag="ACHKA1_000306"
     CDS             complement(66787..67329)
                     /gene="yfcD"
                     /locus_tag="ACHKA1_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO5678012.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(67387..67938)
                     /gene="yfcE"
                     /locus_tag="ACHKA1_000307"
     CDS             complement(67387..67938)
                     /gene="yfcE"
                     /locus_tag="ACHKA1_000307"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO5678013.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(67994..68638)
                     /gene="yfcF"
                     /locus_tag="ACHKA1_000308"
     CDS             complement(67994..68638)
                     /gene="yfcF"
                     /locus_tag="ACHKA1_000308"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO5678014.1"
                     /translation="MSKPAITLWSDAHCFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            68774..69421
                     /gene="yfcG"
                     /locus_tag="ACHKA1_000309"
     CDS             68774..69421
                     /gene="yfcG"
                     /locus_tag="ACHKA1_000309"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO5678015.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            69478..69840
                     /gene="folX"
                     /locus_tag="ACHKA1_000310"
     CDS             69478..69840
                     /gene="folX"
                     /locus_tag="ACHKA1_000310"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO5678016.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            69861..70754
                     /gene="yfcH"
                     /locus_tag="ACHKA1_000311"
     CDS             69861..70754
                     /gene="yfcH"
                     /locus_tag="ACHKA1_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO5678017.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCTRWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(70802..71692)
                     /gene="rpnB"
                     /locus_tag="ACHKA1_000312"
     CDS             complement(70802..71692)
                     /gene="rpnB"
                     /locus_tag="ACHKA1_000312"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO5678018.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLTAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(71889..72662)
                     /gene="hisP"
                     /locus_tag="ACHKA1_000313"
     CDS             complement(71889..72662)
                     /gene="hisP"
                     /locus_tag="ACHKA1_000313"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO5678019.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGAIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(72670..73386)
                     /gene="hisM"
                     /locus_tag="ACHKA1_000314"
     CDS             complement(72670..73386)
                     /gene="hisM"
                     /locus_tag="ACHKA1_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5678020.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(73383..74069)
                     /gene="hisQ"
                     /locus_tag="ACHKA1_000315"
     CDS             complement(73383..74069)
                     /gene="hisQ"
                     /locus_tag="ACHKA1_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO5678021.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(74159..74941)
                     /gene="hisJ"
                     /locus_tag="ACHKA1_000316"
     CDS             complement(74159..74941)
                     /gene="hisJ"
                     /locus_tag="ACHKA1_000316"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO5678022.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQKIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(75162..75944)
                     /gene="argT"
                     /locus_tag="ACHKA1_000317"
     CDS             complement(75162..75944)
                     /gene="argT"
                     /locus_tag="ACHKA1_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO5678023.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(76210..76779)
                     /gene="ubiX"
                     /locus_tag="ACHKA1_000318"
     CDS             complement(76210..76779)
                     /gene="ubiX"
                     /locus_tag="ACHKA1_000318"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO5678024.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(76874..78391)
                     /gene="purF"
                     /locus_tag="ACHKA1_000319"
     CDS             complement(76874..78391)
                     /gene="purF"
                     /locus_tag="ACHKA1_000319"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO5678025.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(78428..78916)
                     /gene="cvpA"
                     /locus_tag="ACHKA1_000320"
     CDS             complement(78428..78916)
                     /gene="cvpA"
                     /locus_tag="ACHKA1_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO5678026.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(79357..80019)
                     /gene="dedD"
                     /locus_tag="ACHKA1_000321"
     CDS             complement(79357..80019)
                     /gene="dedD"
                     /locus_tag="ACHKA1_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO5678027.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(80009..81277)
                     /gene="folC"
                     /locus_tag="ACHKA1_000322"
     CDS             complement(80009..81277)
                     /gene="folC"
                     /locus_tag="ACHKA1_000322"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO5678028.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKTLPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(81347..82261)
                     /gene="accD"
                     /locus_tag="ACHKA1_000323"
     CDS             complement(81347..82261)
                     /gene="accD"
                     /locus_tag="ACHKA1_000323"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO5678029.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(82417..83076)
                     /gene="dedA"
                     /locus_tag="ACHKA1_000324"
     CDS             complement(82417..83076)
                     /gene="dedA"
                     /locus_tag="ACHKA1_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5678030.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(83159..83971)
                     /gene="truA"
                     /locus_tag="ACHKA1_000325"
     CDS             complement(83159..83971)
                     /gene="truA"
                     /locus_tag="ACHKA1_000325"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO5678031.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRFDLPKPPMGPLFLAD"
     gene            complement(83971..84984)
                     /gene="usg"
                     /locus_tag="ACHKA1_000326"
     CDS             complement(83971..84984)
                     /gene="usg"
                     /locus_tag="ACHKA1_000326"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO5678032.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(85050..86186)
                     /gene="pdxB"
                     /locus_tag="ACHKA1_000327"
     CDS             complement(85050..86186)
                     /gene="pdxB"
                     /locus_tag="ACHKA1_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO5678033.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKKAGIGFSAAPGCNAIAVVEYVLSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRNLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHIALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            86285..87280
                     /gene="flk"
                     /locus_tag="ACHKA1_000328"
     CDS             86285..87280
                     /gene="flk"
                     /locus_tag="ACHKA1_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO5678034.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDIQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(87277..88455)
                     /gene="yfcJ"
                     /locus_tag="ACHKA1_000329"
     CDS             complement(87277..88455)
                     /gene="yfcJ"
                     /locus_tag="ACHKA1_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5678035.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFSGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(88764..89984)
                     /gene="fabB"
                     /locus_tag="ACHKA1_000330"
     CDS             complement(88764..89984)
                     /gene="fabB"
                     /locus_tag="ACHKA1_000330"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO5678036.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            90143..92149
                     /gene="mnmC"
                     /locus_tag="ACHKA1_000331"
     CDS             90143..92149
                     /gene="mnmC"
                     /locus_tag="ACHKA1_000331"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO5678037.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHLHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     ISQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEVPALGASGNRQGALYPLLSKHDEALNRFFSNGFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLKLAQQQGLQIYYQYQLQDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDISEKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLVEQKDKAVSAPVYDDLFMLDALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(92270..92548)
                     /gene="yfcL"
                     /locus_tag="ACHKA1_000332"
     CDS             complement(92270..92548)
                     /gene="yfcL"
                     /locus_tag="ACHKA1_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO5678038.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(92582..93130)
                     /gene="epmC"
                     /locus_tag="ACHKA1_000333"
     CDS             complement(92582..93130)
                     /gene="epmC"
                     /locus_tag="ACHKA1_000333"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO5678039.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(93130..93939)
                     /gene="yfcA"
                     /locus_tag="ACHKA1_000334"
     CDS             complement(93130..93939)
                     /gene="yfcA"
                     /locus_tag="ACHKA1_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO5678040.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(93939..94763)
                     /gene="mepA"
                     /locus_tag="ACHKA1_000335"
     CDS             complement(93939..94763)
                     /gene="mepA"
                     /locus_tag="ACHKA1_000335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO5678041.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKRVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(94767..95852)
                     /gene="aroC"
                     /locus_tag="ACHKA1_000336"
     CDS             complement(94767..95852)
                     /gene="aroC"
                     /locus_tag="ACHKA1_000336"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO5678042.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(95887..96819)
                     /gene="prmB"
                     /locus_tag="ACHKA1_000337"
     CDS             complement(95887..96819)
                     /gene="prmB"
                     /locus_tag="ACHKA1_000337"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO5678043.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            96985..97536
                     /gene="smrB"
                     /locus_tag="ACHKA1_000338"
     CDS             96985..97536
                     /gene="smrB"
                     /locus_tag="ACHKA1_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO5678044.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(97650..98489)
                     /locus_tag="ACHKA1_000339"
     CDS             complement(97650..98489)
                     /locus_tag="ACHKA1_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO5678045.1"
                     /translation="MKIKVILFGMLLFGLNSSVQADVTFNNVQTYGASYGSGLTTSIV
                     YKIDVLTPEGAYYGTYSQGGIMVQAGVVPNLTWTGPGVPPDITIPPGRAPIVDDSYCP
                     GMTSGWVCHRLPVNVHVTGTTHGCPWVVTTSIYSTARPPQAGPYYYQGPNAHQSNCPT
                     EPVASYDISWDENTVVHNKSLSLQSTGGVIEKSLPTFLMKDGRLCDGSDFSDDRGAYC
                     RLVSQLMTFSVQGCDNNKVNVTANRHPVTDKQLHDMQVRVDTSERQSFDATCRFQYIL
                     NEL"
     gene            complement(98490..99008)
                     /locus_tag="ACHKA1_000340"
     CDS             complement(98490..99008)
                     /locus_tag="ACHKA1_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5678046.1"
                     /translation="MKTWFALIVMLLLAGNSTARADNNLHFYGNLLSKSCTLVVQGQV
                     LAEVHFPSISSRDLMSREQSDRVPVVFQLKDCKGPVGYKVRVTLTGTEDSDLPGFLAL
                     DASSGAQGVSIGMETVDGIQVPINNSAGAIFNLSNGANNLNFKAWLQAKSGREVTSGE
                     FTASLTAIFEYI"
     gene            complement(99011..99487)
                     /locus_tag="ACHKA1_000341"
     CDS             complement(99011..99487)
                     /locus_tag="ACHKA1_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5678047.1"
                     /translation="MKILLYLLLCMSSGIVNASPDITFKGTLVLPPACTISDGNTIEV
                     EFRDVIIDSIDGNNGREVVPYDIKCDAVTPGSSWDMTLTWIGTQTSYDDAAIETDVTG
                     LGIKLRQNGQPFRVNTPIQINADTKPQLEAVPVKAVDAVLTDGTFSASATLRVEYQ"
     gene            complement(99484..99987)
                     /locus_tag="ACHKA1_000342"
     CDS             complement(99484..99987)
                     /locus_tag="ACHKA1_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5678048.1"
                     /translation="MRQIFVVGVGILLLGVSGIYNIILANSKPVDLILQVHVDPPPPC
                     SVTGSSVDFQTVNINDINGSNYRKDAGYTLSCPDRKADDLRMQLRGNTTTINGEKVVD
                     VGVPGFGIRIENADNNSLFSIGENNWTPFNITSQPKLNAVPVKQSGAQLSGADFSGSL
                     TMVVDYQ"
     gene            complement(100003..100755)
                     /locus_tag="ACHKA1_000343"
     CDS             complement(100003..100755)
                     /locus_tag="ACHKA1_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5678049.1"
                     /translation="MTNLFCSAKKSTMLLALLLTTASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDEQGNRISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKV
                     TLTRSGQEFTVNNPTPYYVVISNASTQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPV
                     LSYVNDYGARMPLYFKCGGDTCQVDVEQSRKG"
     gene            complement(100778..103417)
                     /locus_tag="ACHKA1_000344"
     CDS             complement(100778..103417)
                     /locus_tag="ACHKA1_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO5678050.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAEDEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     LHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDVTHSHTRLDKETAYGK
                     GSLDGNSFRVSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNIMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQSDKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQATLTEGAIGYRKFSVISGQKAMAVLRLQDGSYPPFGAEVKNDSAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHEI
                     KPMIQEQTQQVMPTEAPVSVSAN"
     gene            complement(103499..104062)
                     /gene="yfcV"
                     /locus_tag="ACHKA1_000345"
     CDS             complement(103499..104062)
                     /gene="yfcV"
                     /locus_tag="ACHKA1_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5678051.1"
                     /translation="MSKFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTN
                     SGNYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        25786 a        28025 c        25847 g        24452 t
ORIGIN      
        1 tatcgatatc accgacaaaa agttagcacc attaataaat gatagtttca gctatacacg
       61 agatttcttt gcttactcaa aacaggaaaa aaatatcttc acatttgata attctaagtt
      121 tgtagatcca aaggaaaaag aaggtttaat ggtacaacac agcaatgggc aactggttat
      181 tactggtaaa tattgccctg aaggtgtgca aacagcattc acccaagaac aatatgataa
      241 attaataaga tacattaata ttttctttac ctttccgaaa ttagaataat actttacagt
      301 ataaactgca ttacaattta ttattgagac ctattcatac tgtttaaaga ataacaatgc
      361 ccagtgttag cttttttaac gaatgagttt tacgaaaatt atgttggcat cttttttaca
      421 ggatgcaccg gttaatgaaa agaataaccg gtagtagcat tatttcgctt tagcagagga
      481 tggcgcatga acgccatcct gcagtagcag cattactctt tattaaggaa tttaaccgct
      541 ttatcaggga aatcagtaaa caacccattt acacccgctt tgttatacag agcatcatat
      601 aactgattca catcagttgt gtattcaggc agtttatctg accgcacggt ataaggatgc
      661 actaccagct tgttctgctg agcatcttgc accatgccag tgagtttgat attacccggc
      721 tgcgatgtct cctcaatcaa catatggtaa tccggaccaa taccatctgc atattccgcc
      781 acctgtttca tggcacccgg cttaaacatc cagtcgtagt tgtaattaac ccagcttccg
      841 tccggctgtt tctgctgcgt ttcattccag tcggtatagg caatcagctg taccagattg
      901 agatccatgc ccattttggg ttccagctca ttcttaatac gcttcagctc atcagcatca
      961 aaacattgca aataaacttt atcgtcttta ccggtgtaac catatttctt cagcacttcc
     1021 agcgtttttg ccgcaatatc cttcccttcc tgatgatgga accacggcgc tttgatttct
     1081 ggatagatac cgatattttt cccggtagag tgatttaacc cctgaacaaa ttcaatctct
     1141 tcttcaaagg tgtgtacccg gaagtcggac ttacccattg ggaaacgccc tggataagtc
     1201 tgcacttttt taccgttttc aatatcgaaa ccttcggtaa atttcaacga cttaatttca
     1261 tccagcgtga aatctatcgc gtagtaacga ccgtctttgc gcgcccgatc cgggaaacga
     1321 tcggcaacat cagtaacacg atcgaggtaa tggtcatgca gaacaaccag atggtcgtct
     1381 ttggtcatca ccaaatcctg ttccagataa tccgctccct gcgcatacgc catcgctttt
     1441 gctggcagcg tatgctccgg caaatatcca ctggcaccgc gatgggcgat gactattttt
     1501 tcgttgctgt ccgccgccat tgcactgctt cccatgacta tagtgctcat catgatcgcc
     1561 atgctaaggt ttttcagcgt caatttcatg ccattagcct ccgttgcgtt tttgcagtaa
     1621 ttcctgatgg cgacgttttt cgccaatcat cacaacaatc aacaagataa ccgccagaat
     1681 gctgccgcca atcattacca taaagccgcc atcccagccg aagaagtcca cggtgtagcc
     1741 aacaatcgcg ctcgccgcca ccgaaccgcc caggtaacca aacagaccgg taaagcccgc
     1801 tgccgttcct gccgcttttt tcggtgccag ttccagcgca tgcagaccga tcagcatcac
     1861 agggccgtag atcaggaagc cgataacaat catacaaatc atatcgacgg ttgggttacc
     1921 tgccgggttc atccagtaaa cgatagtcgc gatggtcacc agtgtcataa agaaaacgcc
     1981 ggttgcccca cggttgccac ggaagacttt atccgacatc cagccgcaca gcagggtgcc
     2041 cggaataccc gcatactcat agaagaagta ggcccaggag gatttatcca gcgcgaaatg
     2101 cttaacctct ttcagataag tcggtgacca gtcgaggatg ccgtaacgca gcagataaac
     2161 gaacacgttg gcgatggcga tgtaccacag cagtttgttc ggcagcacgt actgcatgaa
     2221 gatttgcttc gcggtcagct cctgttccgc tttttcgtta tagtcgtccg gataatcatt
     2281 tttgtactct tcgatcggcg gcaagccaca ggattgcggg gtatcgcgca tcatcgcaaa
     2341 ggcgaataat gccaccagaa tggcgcaaaa agcgggcata tagagcgccg cgtgccagtc
     2401 attgaaccag gccatcccca gcaggaacag cagcggcgga ataccaccac cgacgttgtg
     2461 cgcacagttc cacactgaca caatgccgcc acgttctttc tgcgaccacc agtgcaccat
     2521 agtacgacca cacggcggcc accccatccc ctggaaccaa ccgcagagga acaacagtac
     2581 aaacatcacc gcaatgctcg acgtcgccca tggcacaaag cccataaaca acatcactgc
     2641 cgccgccaga atcaaacctg cgggcaggaa aacgcgcgga ttcgagcgat ccgataccga
     2701 acccatgatg aatttcgaaa atccataagc aatcgagatc cccgaaaggg caaaacctaa
     2761 atcaccgcgt gagaatccct gctcaaccag ataaggcata gcaagcgcaa agttcttacg
     2821 aaccaaatag taagcagcat agccaaagaa tatccccagg aaaatttgcc agcgcaatcg
     2881 acgataagtc ggatcgatct ccgcggcagg taagcgcgct ttgtgtggcg ctggtttaaa
     2941 aatactcaac attgatagcc tccgtggccc gtggtcttat ttatgattaa cagcctgatt
     3001 cagtgagaga acctgccgtt tcttgagttg ccgcgatgtt aagaaaacat tcataaatta
     3061 aatgtgaatt gccgcacaca ttattaaata agatttacag aatgttcaaa atgacgcatg
     3121 aaatcacgtt tcactttcga attatgagcg aatatgcgcg aaatcaaaca attcatgttt
     3181 ttactatggc taaatggtaa aaaacgaact tcagagggat aacaatgaaa actcgcgact
     3241 cgcaatcaag tgacgtgatt atcattggcg gcggcgcaac gggagccggg attgcccgcg
     3301 actgtgccct gcgcgggctg cgcgtgattt tggttgagcg ccacgacatc gcaaccggtg
     3361 ccaccgggcg taaccacggc ctgctgcaca gcggtgcgcg ctacgcggta accgatgcgg
     3421 aatcggcccg cgaatgcatt agtgaaaacc agatcctgaa acgcattgca cgtcactgcg
     3481 ttgaaccaac caacggcctg tttatcaccc tgccggaaga tgacctctcc ttccaggcca
     3541 cttttattcg cgcctgcgaa gaagcaggga tcagcgcaga agctatagac ccgcagcaag
     3601 cgcgcattat cgaacctgcc gttaacccgg cactgattgg cgcggtgaaa gttccggatg
     3661 gcaccgttga tccatttcgt ctgaccgcag caaacatgct ggatgccaaa gaacacggtg
     3721 ccgttatcct tactgctcat gaagtcacgg gactgattcg tgaaggcgca acggtgtgcg
     3781 gcgttcatgt acgcaaccac ctcaccggcg aaacccagtc gctacatgcg cctgtcgtgg
     3841 ttaacgctgc cgggatctgg gggcaacaca ttgccgaata tgccgatctg cgcattcgca
     3901 tgttcccggc gaaaggatcg ctgctgatca tggatcaccg cattaaccag catgtgatca
     3961 accgctgccg taaaccttcc gacgccgata ttctggtgcc tggcgatacc atttcgctga
     4021 ttggcaccac ctctttacgt attgattaca acgagattga cgataatcgg gtgacggcag
     4081 aagaagtgga tatcctgctg cgtgaagggg aaaaactggc ccccgtgatg gcgcaaacgc
     4141 gcattttgcg ggcctactct ggcgtgcgtc cactggtcgc cagcgatgat gatccgagcg
     4201 ggcgtaacgt cagccgcggc atcgtgctgc tcgatcatgc tgaacgcgat ggtctggacg
     4261 gatttatcac cattaccggt ggcaaactga tgacctatcg gctgatggcg gaatgggcta
     4321 ccgacgccgt atgccgcaaa ctgggcaaca cgcgcccctg tacgaccgcc gatctggcac
     4381 ttccgggttc acaggaaccc gctgaagtta ccttgcgtaa agtcatctcc ctgcctgccc
     4441 cgctgcgcgg ttctgcggtt tatcgtcatg gcgatcgtac gcctgcctgg ctgagcgaag
     4501 gccgtctgca ccgtagcctg gtatgtgagt gcgaagcggt aactgcgggt gaagtgcagt
     4561 acgcggtaga aaatttaaac gttaatagcc tgctggattt acgccgtcgt acccgtgtgg
     4621 ggatgggtac ctgccagggc gaactctgcg cctgccgcgc tgccggactg ctgcaacgtt
     4681 ttaacgtcac gacgtccgcg caatctatcg agcaactttc caccttcctt aacgaacgct
     4741 ggaaaggcgt gcaacccatc gcctggggag atgcactgcg cgaaagcgaa tttacccgct
     4801 gggtttatca gggattgtgt ggtctggaga aggagcagaa agatgcgctt tgatactgtc
     4861 attatgggcg gcggcctcgc cggattactc tgtggcctgc aactgcaaaa acacggcctg
     4921 cgctgtgcca ttgtcacgcg tggtcaaagc gcgctgcatt tctcatccgg ttcgctggat
     4981 ttgctgagcc atctgccaga tggtcaaccg gtgacagaca ttcacagtgg actggaatct
     5041 ttgcgtcagc aggcacctgc acatccttac tctcttctcg ggccacaacg cgtgctcgat
     5101 ctcgcttgcc aggcgcaggc attaatcgct gaaagcggtg cgcaattgca gggcagcgta
     5161 gaactggctc accagcggat tacgccgctc ggcactctgc gctctacctg gctaagttcg
     5221 ccagaagtcc ccgtctggcc gctgcccgcg aagaaaatat gtgtagtggg aattagtgga
     5281 ctgatggatt ttcaggcgca ccttgcggca gcttcgttgc gtgaactcgg ccttgccgtt
     5341 gaaaccgcag aaatagagct gccggaactg gatgtcctgc gcaataacgc caccgagttc
     5401 cgcgcggtga atatcgcccg tttccttgat aatgaagaaa actggccgct gttacttgat
     5461 gcgcttattc ctgtcgccaa tacctgcgaa atgatcctga tgcccgcctg cttcggcctg
     5521 gctgatgaca aactgtggcg ttggttgaat gaaaaactac cttgttcact gatgcttttg
     5581 ccaacgctgc cgccttccgt gctgggcatt cgtctgcaaa accagttaca gcgccagttt
     5641 gtgcgtcagg gtggcgtgtg gatgccgggc gatgaagtga aaaaagtgac ctgtaaaaat
     5701 ggcgtagtga atgaaatctg gacccgcaat cacgccgata tcccgctgcg tccacgtttc
     5761 gcggttctcg ccagcggcag tttctttagt ggcggactgg tagcggaacg taacggcatt
     5821 cgagagccga ttctcggcct tgatgtgcta caaaccgcca cgcggggtga atggtataag
     5881 ggagattttt ttgcgccgca accgtggcag cagttcggtg taaccactga tcagacgcta
     5941 cgcccgtcgc aggcagggca aaccatcgaa aacctgtttg ccatcggttc ggtgctgggc
     6001 ggatttgatc ccatcgccca gggatgcggc ggcggtgttt gtgccgtcag tgctttacat
     6061 gccgctcaac agattgccca acgcgcagga ggccaacaat gaatgacacc tgcttcgaaa
     6121 actgcattaa gtgcaccgtc tgcaccaccg cctgcccagt gagccgggtg aatcccggtt
     6181 atccagggcc aaaacaagcc gggccggatg gcgagcgtct gcgtttaaaa gatggcgcac
     6241 tgtatgacga ggcactgaaa tattgcatca actgcaaacg ttgcgaagtc gcctgcccgt
     6301 ccgatgtgaa gattggcgat atcatccagc gcgcacgggc gaaatatgac accacgcgcc
     6361 cgtcgctgcg caattttgtt ttaagccata ccgacctgat gggtagcgtt tccacgccgt
     6421 tcgcaccaat cgtcaacacc gctacctcgc tgaaaccggt gcggcagttg cttgatgcgg
     6481 cgttaaaaat cgatcatcgc cgcacgctac cgaaatactc cttcggcacg tttcgtcgct
     6541 ggtatcgcag cgtggcggcc cagcaggcgc agtataaaga ccaggtcgcc tttttccacg
     6601 gctgcttcgt taactacaac catccgcagt taggtaaaga tttaattaaa gtgctcaacg
     6661 cgatgggtac tggcgtgcaa ctgctcagca aagaaaaatg ctgcggtgtg ccgctaatcg
     6721 ccaacggctt taccgataaa gcacgcaaac aggcaattac gaatgtagag tcgatccgcg
     6781 aagccgtggg agtaaaaggc attccggtga ttgccacctc ctccacctgt actttcgccc
     6841 tgcgcgacga gtacccggaa gtgctgaatg tcgacaacaa aggcttgcgc gatcatatcg
     6901 aactggcgac ccgctggctg tggcgcaagc tggacgaagg caaaacgtta ccgctgaaac
     6961 cgctgccgct gaaagtggtt tatcacactc cgtgccatat ggaaaaaatg ggctggacgc
     7021 tctacaccct ggagctgttg cgtaacatcc cggggcttga gttaacggtg ctggattccc
     7081 agtgctgcgg tattgcgggt acttacggtt tcaaaaaaga gaactacccc acctcacaag
     7141 ccatcggcgc accactgttc cgccagatag aagaaagcgg cgcagatctg gtggtcaccg
     7201 actgcgaaac ctgtaaatgg cagattgaga tgtccacaag tcttcgctgc gaacatccga
     7261 ttacgctact ggcccaggcg ctggcttaaa ctcctttctg atgcccggta agcatgtggt
     7321 taccgggcat ttttgcgtac acgattccgt gcccaatgta tgcgttgcaa cgcagtgaaa
     7381 attcctctga aaacgtctcg caaaggctga aactggcaga tgtcaaaggc ctgggataac
     7441 cgtaatgtcg cgtcatcata aatatcaggt gacggacaac catgaccgaa tcaacaacct
     7501 cctccccgca tgatgcagta tttaaaacct ttatgttcac acccgaaacc gcacgggatt
     7561 ttctcgaaat acatctacca gagccgctgc gcaagctttg caacctgcaa accttacgcc
     7621 tggaacccac cagttttatt gaaaaaagtt tacgcgctta ctactcggat gttttgtggt
     7681 ccgtggaaac cagcgacggt gacggctata tctactgcgt gattgaacat caaagctctg
     7741 cagaaaagaa tatggctttt cggctaatgc gctatgccac tgccgccatg cagcgtcacc
     7801 tggacaaagg ctatgacaga gtcccgctgg tggtgccgtt gctgttttat catggcgaaa
     7861 cctcgcccta cccgtactca ctcaactggc tggatgagtt tgacgatccg caacttgccc
     7921 ggcagttgta caccgaagct tttccgttgg tggatattac catcgtacct gacgatgaga
     7981 tcatgcaaca tcggcgtata gctctgcttg aactgattca aaagcatatt cgcgaccgcg
     8041 atttaatcgg tatggtcgac aggatcacca cgcttttggt tagaggcttc actaatgaca
     8101 gccagctaca aacactgttt aattatctgc tgcaatgcgg cgatacctcc cgtttcaccc
     8161 gttttattca ggagattgcc gaacgttcac tactacaaaa ggagagatta atgactattg
     8221 ctgaacggct acggcaggaa ggacatcaaa ttggttggca ggaaggtaaa ttagaaggtt
     8281 tgcagcaagg taaattggag ggtctgcaga aaggtaaatt agaaggtttg caggaaggca
     8341 tgcatgagca agccattaaa attgccttgc gcatgctgga gcagggcttt gaacgtgaga
     8401 ttgtgctggc ggcaacccaa ctcactgatg ctgatattcc gaactgtcat taaaaggaga
     8461 gattgatgac tattgctgaa cggctacggc aggaaggaca tcaaattggc tggcaagaag
     8521 gtaaattagt aggtttgcag caaggtaaat tagaaggttt gcaggaaggc atgcatgaac
     8581 aagctattaa aattgccttg cgcatgctgg aacagggcat tgatcgtgac caggtgctcg
     8641 cggccaccca gctaagcgaa gccgatctgg cagcgaataa ccactaatta acacaggccc
     8701 acagccgatc cccatgggcc tttgatatca ataactacct tttatgcgtg gcccattttt
     8761 gcctgattta aacatcgcca gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac
     8821 gccaacagcg acaaagttcg ctccccacgc caggcattgc tgcgccatat caggagccac
     8881 agccagaaaa ccagccgctt tacccgcagc acggatccgc cgaatgctgg tttcaataat
     8941 tcgctgcact tccgggtgcc cggcgttatc cgggtagccc aacgacgcag aaagatccgc
     9001 aggtccaata aacacgccat caatcccttc gacgtcgagg atctcgtcca ggttatccag
     9061 tgccgtttta ctttccacct gcactaacag acaaagcgaa tcgttgactt gcgccatgta
     9121 attctcaatg cgtccccagc gcgcagcccg tgccacactg gccccaacac cacgttcacc
     9181 gtagggagga tagcgcgtgg cagacaccac ctgacgtgcc tgatcggcag tatcgaccat
     9241 cgggatcagt agagtttgcg cgccaatatc caggacttgt ttaatcagcg acttactgcc
     9301 atccaccgga cggatcacgg gttggctggc atagggcgct accgcctgta gctggtgata
     9361 aagatcctga atggtgtttg gcgcgtgctc cccatcaatc agcaaccagt cataaccgga
     9421 agtggcggca atttctgcca tataggcagt tgttgagctt aaccacagac caatttgcac
     9481 ttcgcccttg cgtaaacgtt ctttaaaggg attggttaat aatgcgttca tcgtgttcct
     9541 taacctattt aatgatgtgc cacgtcggtc tgcgcaacag tacgatttac acgcagagtg
     9601 aaaataatca gcgaaccgat gaccgccacc gctgccagcg tcagtaatcc cgccgcatcg
     9661 ctggaaaata acgtttctgc tttcacgcgc aggatcgggg caataaaccc gcccactgca
     9721 ccaaacaggt ttacaaaacc aatccccgca gccagcgccg taccggaaag cagttgtgtc
     9781 ggcatcgtcc agaacaccgg ctgcacggca ataaacccga tggctgcaac acacagcgct
     9841 acgatcgcca ttactggaga aagcagcccg gacagaccaa taccaatgcc cgccgccagt
     9901 aatgtcagcg ctgcgacatt acgccgttcg ccggttttat cggaatagcg cggaataagc
     9961 caggtcccaa acaaggccgc aacccacgga atggcggtga ccaccgacgc tgtaaagccc
    10021 acttttgttc ccagcaatgc cgcaacctgg gtcggcagga agaaaattaa tccgtaaacc
    10081 gccacctgaa tggtcaggta gataatcgcc agttgccaga ctcgcccatt acgcagcgca
    10141 tcgctcagcc gagaagtcac tttctgttgt tcttcacttg ccagttgatt gataagcaac
    10201 gttttttctt gtttactcag aaaacgtgcc tgctccggtg tgtcatcaag ccaaaagaat
    10261 gtgaataccc cagcgccgac tgccaacaat ccttcaatca caaacatcca gaaccagccg
    10321 ggatgcccca taaatccatg catctccagc agcgcgccgg aaagcggtga tcccagtgtt
    10381 aacgccagcg gtgcgcccat atagaacagc cccataatgc tggcgcgatt acgctgcgga
    10441 aaccattgcg aggtgagata aatcatacca gggaaaaatc cggcctccgc agcaccaagc
    10501 agagtgcgaa ctatcagaaa tttcgcttca gtatcggccc atgccatggc tgccgaaaga
    10561 aatccccaca gcagtgttgt cgtaccaatc caggttctgg cccccagttt gcgcatcaaa
    10621 agattcgccg gaacacccag aaacgcatat accacaaaga aaatgcctgc tcccagcgca
    10681 taagcttcat tactcaaccc ggtatcaatc tggtaggtct gtttggcaaa accgatattc
    10741 gaacggtcga gaaacgccag cacatacagc gccaacataa acggaattaa acgcacacgg
    10801 tttttcttca ccacggcgtc aagcaaagcg gtgctcataa aaagctcctt agaatatgtg
    10861 agcgttaccg ttgtcacagc aacgcccgga gtgattagtg gctgtagggg cgtttcaaat
    10921 tgcagtcacg attgagttcg acgccaaaac cgggtttatc aagcactgat ttatgaatac
    10981 gaccattcac cggaaccggc tcattgagca gaatcgggtc aaactgcgga cgcatcgttg
    11041 aacaatccgg actggtcatc aggaattcgc tgaatggcgt attagtgaag gtgatcaccg
    11101 catggtggga gtaaacagac gaaccgtgcg gcaccaccag ttgccccctg gatttggcga
    11161 ttgcggcaat ttccaccagc gtggttaagc caccgcacca gccaacatcc ggctgcataa
    11221 tgtcgatacc ggtttctgaa agcgtacgaa aagattgcag tgtgccgtgg tgctcaccgc
    11281 tggtgaccat cattccgact ggcgcgttgc gtttcagttc gcgataacct tcatactgct
    11341 gtggcggcag gcactcttcg atccatttca ggttataggg cgcgcaagcg tgggccagtt
    11401 tggtcgcata gttcacgtcc tgactcatcc agcagtcgag cattaaccag aaatcctcac
    11461 cgcatttttc acgcatatcc gcgaccatag cggcatcttt gcggatcccc gcatcgccat
    11521 catgtggccc ccagtgcgtc ggcattttgc caccgataaa gcccatctct tttgccagat
    11581 ccggacgcgc acctgtggcg tagaactgaa tctcatcacg aacagcgccg cctaaaagtt
    11641 tataaaccgg aagcccgacc actttgccga acagatccca cagagccaga tcgacacagg
    11701 aaatcgtgtt catcaccagg ccaccagagc cggagtaata cagggtggca ttgagcattt
    11761 gatcatggat cagtttgata tcactgacac atttaccctc aatgaaacgg ttaaggtgtt
    11821 tttcgacgat aaaacagccc atttcaccgg ctgtcgaaac ggcgaatccg gtctggccgt
    11881 tttctgcttc gacttcaaca accaacgtgc caagaacgtt aatgccaaat gactggcgtg
    11941 actgctcgta atcgcggtat ttactcatcg gggtggcaat atgatcgtct atccagtgat
    12001 tcgccccctg gtcgtgataa tcaccgccgc cagcgccttt ttctgctgtc gcaccgccag
    12061 taaaccaggc gcgaacctgt ttaatttttg gtagggtcat gatgttctcc attgttatga
    12121 ggcttgtaag tcaaagggac ttttccatcc caacagacgt gaaatatccc tggcgcaggc
    12181 aatggccttg cccgccagat aatcacggta ttcttcattg atttgtaagc gggtaccgac
    12241 caccgagatc gcagcggtaa gctcgttatt ggcgttaaac accggcgcag cgacacaacg
    12301 gacatcggcg taatcttcgc cgttgtcata gctccagccc tgacggcgaa tacgcgccag
    12361 ttcttcgtga agttgctgtg gatgagtaat cgttgtgggt gtcgcctgct cccagaccag
    12421 cccttcgata atactttgct gtacagttgc aggttgccag gcaagcaggc atttacctat
    12481 tccggagcga taaagcgaaa ggctttttcc ttcatgtgaa cgcacgctga tagtggctga
    12541 tgactccact ttcaaaatgt aataagcgct gccgttatca ataatcccca ggtgacataa
    12601 cagcccggtg gtatccatca gttgcgtcaa acgcggtcga gccagttccc ggagatccat
    12661 cttgcttaac gcatgaccgg aaagctccac cagcctggtc cacaaacaaa aattttcctg
    12721 gtgatcaagg ctaagaaaac gctgacgcct gagttcatta agcagcaaat aggccgtgct
    12781 tttagggatc cccaatgtgt caatgatcgt cgcagcatta caggggccaa tgcgggcaat
    12841 caaattgaga atatcaatag cccgtgtcag ggcgggaact ttgcttgatt ccaacatact
    12901 ggactccagt cttagatact ggaatcagtc tcgttgctca gggggagtaa aattgtgaag
    12961 cgcatcaaat cccgctcctc gtccaacgga ctggagaaat tacgcacaaa tttgtgaatc
    13021 agcgcacgga ttttttctta ttgagtacaa caggttggac aaattttgcc ggagggggaa
    13081 tgtgccggtg cagtaaaccg gcacagagtg gcggaatcag acggataagg tcatggactc
    13141 aacaacctca atccagccat gctcactggc gatgtcctgg ccgtttaacc aacggcgcag
    13201 catattcagt gccatcattg cgcacacttc ctgacggata gccaggctgt agcgtgtagt
    13261 gctgaaacgc acacgcagag cgaaagtgcc gtctggcgtg gctagcgcaa agttgagatg
    13321 ctcgttctcg aaacccgaaa cagccagtgc cagcccggca aaatggttgg cccgccgttc
    13381 tgtaatccag tgcgcagttt gcgccagggt ttcctcctgt gaaggaacca cttcacacgc
    13441 cagcaatgga gcacctgcgc gagaaagttg caaagccaat aaaccaccgg tgaattgctc
    13501 gctcaacgtc aggctgaact ggcggttttg caattcgcga ctgatctgcg cgggcagtcc
    13561 ttcggtgcct tcgaaaatca cgctctgtcc ggcaacacgt ttaacgtcca gccacagttt
    13621 ttccatcgcc tgttgctcgc ttgccggtcc ggtgagtttc agttcgatga tcggcattga
    13681 ggagcgatag cccattgtta cgcccggcgg cagttgtaga gtgtccaggc tttgtgccag
    13741 atcgctttcc gaacgaccaa aggtagtcaa acgcaggcaa accggcggct gtggtaaaga
    13801 aaaacgctcg cgcagacgcg gcaggatttc gtgctcgacc atcaccttaa attctgacgg
    13861 tacgccggga gtaaagaaca tcaggcaacg attaagctgc acggcaaaac cacaggcggt
    13921 gcctaccggg ttattgataa attcagcact ggcaggcagc tccgcttgtt tacggttgct
    13981 cggtgccatt actcgtccac gttcgtggaa atagcgttcc atctctttga gccaggcttc
    14041 atgcagcacc aggccttcac cttttgctgt cgcagcggcg agtgcgctta aatcatcact
    14101 ggtcggcccc agcccgccgt taacgatcag cacatcggcg tgctgactac gttcgcgaag
    14161 aatggtgact aagtcatcaa ggttatcccc caccgtattg cggcgagata atggcaaccc
    14221 ctgatgaaag aaaaaatcgg ccagccaggc agcgttagtg tcaacgattt gcccgtgtaa
    14281 cacttcatcc ccggtggata gcatttccac ttttaacatg gttatctcct gcttttgtta
    14341 tcgaatcact ataacgcaaa gggagataag gcgtagtaag aaactgaatg gcgcgacaga
    14401 atgccgcgcc ggaagggatc agaaactggc gttaacccca acatacgggc cgtcagccac
    14461 ggcgttgtcg cggttaccgt ctttacccga cagattcagg tagcgataac ccgcctcaat
    14521 actgactgga cgcataatgg tgtaacgcgc gccagcattc gcttcctcat aacttttaat
    14581 accgctggag agtgaatccg gagagtagta atactcgcca aacaaacgga agctgttgcc
    14641 aattttccac tgcaaaccac ctcctaccgc tgcggcataa ccttcatcgc cgtaattcgg
    14701 gttggtgtaa acgccttttc cgccaacggt cgccattaac ggcccgagag gcagattcaa
    14761 ccccagcccc acgcccgcga cgtcaccgtc gtcgtcgtta tgtgtccagt taccgcttaa
    14821 agccaggccc gtcgattcag tcccaaaacc gacaccaata ttggtgtatt ctttacccac
    14881 ctggccgctg atgctcattg cattgaccga tgcagaaacc aacagcattc cggccagacc
    14941 tgctagcgca atttttttca ttatcccctt tcccacaagc aattaatcaa aattcccaaa
    15001 gctgcgggat tgtactgcct ggcgcagagg attcaatgag cgaaaaaagg gatctagtca
    15061 cttattgtaa ctaaatactt cttaaataat taaacgccga aaaaataaac acactttacc
    15121 gatacacaca aaaataataa taagtgcagt tatcatcgca aaagagatta ctgtcacttt
    15181 cacatatttc tgagttaccg tattctcatg gtttctattt atttataagg aatacccctg
    15241 tgcgacaacg gactattgta tgccctttga ttcaaaatga tggtgcttat ttgctgtgta
    15301 aaatggccga cgatcgcggc gttttccccg gtcaatgggc gctttcgggt ggcggcgtgg
    15361 agcccggcga acgaattgaa gaggcactac gccgcgaaat tcgcgaagaa ctgggagaac
    15421 agctgctttt gacagaaatc aagccgtgga ccttcagcga tgatattcgc accaagacgt
    15481 atgcagatgg tcgcaaggaa gagatttata tgatttacct gatttttgac tgcgtttctg
    15541 ccaaccgaga agtgaaaata aacgaagagt ttcaggacta cgcgtgggta aaacctgaag
    15601 atctggtgca ttatgatttg aatgttgcca cccgaaaaac gttacgtttg aaaggtcttc
    15661 tttaacaatg atgacagcca tcactggctg tcagattgaa aatttagtgg ttaacgaatt
    15721 ccccatccag ataaactttg cctttttcca catgcatgac taaaccatcc agataatcag
    15781 gtttaaatgt cgcatcacgc ttatctttag caatatatgt caggcaatga ttatgggtga
    15841 aaataacgat atttttatca ggcgctttgc tttgtaagtc cttaattgca ctataaatct
    15901 cattaccgca ctgaagaagt cgtttatcta ccgtcaattt tttacccgct gaaaaccagg
    15961 tcgccgactg aatggtccgg acggtattac tggaatagag atcgaaatca gggatatcag
    16021 cactaaaagc gttgcccagt tctcgggcat cctgggtacc tttgaccgta atacctgttt
    16081 tatctgacaa gcattgattg gttgaacgat cgcaacgttc agcatggcga aacaaaacga
    16141 caaccgggtg ctgctgtgcc agtctggcca gtgttttatt gtcgatacgt ggcaaaccat
    16201 tgctactcca ggcggcatga gtacccagtc cggcaattgc agcgagcgcc agtaaaatga
    16261 tgaaatattt ttttgacttc aatgatgagc ggcaaaaagc taacacgtaa actccaccta
    16321 tagacaagcg caaccagaca attaccgtga aattgagcga catttctggc gataattagc
    16381 agttggtgta atattaaaaa tcctacgatg tcggcaaaat gcctcaaaat tttgccaaat
    16441 gcaaagccta aataagaaaa aatataaaaa tttcaatatt tacgtctaat attagtttct
    16501 taaggttaag ttaatattct atccttaaaa tttcgctcca aatggcaaaa tatacacaac
    16561 actctttata gcaaatataa gtggacaggt attcaatggc ggaaggaaaa gcaatgtcag
    16621 aatttttgcc tttttcgcga ccagcaatgg gcgtggagga actcgctgca gttaaagagg
    16681 ttctcgaatc cggttggatc acaaccggtc cgaagaatca ggcgcttgag caagcctttt
    16741 gccagttgac gggaaatcag catgccatcg cggtcagttc agccaccgcc ggaatgcata
    16801 tcacgctaat ggcgttggaa attggcaagg gcgatgaagt gattacgcct tccctgacct
    16861 gggtttcaac cctcaatatg atttccttgt tgggtgcaac gccggtaatg gtggatgtcg
    16921 atcgcgatac gctgatggtc acgcctgaag cgatcgaatc agccattacg ccacgcacta
    16981 aagccatcat tccggtgcat tatgccggtg cgccagcaga tattaacgcc attcgcgcca
    17041 ttggcgaacg ttacggcatc gcagttatcg aagatgcagc ccatgccgtc ggtacgtatt
    17101 acaaagggcg acatattggc gcaaaaggta ccgctatttt ttcgtttcac gccatcaaaa
    17161 atattacctg tgcggaaggt ggcctgattg ttactgataa tgaaaatctt gcccgccagc
    17221 tacggatgct gaaatttcac ggtctgggtg tcgatgccta tgacagacaa acctggggcc
    17281 gtgcaccgca ggctgaagtc ttaacaccgg gctataagta caatctgacc gatattaacg
    17341 ccgcgattgc cctgacacag ttagctaaat tagagcacct caacacccgt cggcgcgaaa
    17401 ttgcccagca atatcagcaa gcactggcag ctctcccctt tcagccatta agccttcccg
    17461 cctggtcgca cgttcacgcc tggcatctgt ttattattcg tgtcgatgaa caacgttgtg
    17521 gtatcagtcg aaatgcgttg atggaagcgt taaaagaaag aggtattggt accgggttac
    17581 atttccgcgc cgctcacaca caaaaatatt atcgcgagcg ttttcccacg ctgtcgttac
    17641 cgaataccga atggaatagc gaacgcatct gttcgttgcc gctgttcccg gatatgacta
    17701 ccgccgatgc cgaccgtgtc atcacagccc ttcagcaact cgcaggacaa taagccatgt
    17761 ttgaaatcca ccctgttaag aaagtctcgg tggttattcc cgtttataac gagcaggaaa
    17821 gcttaccgga attaatcagg cgcaccacca cagcctgtga atcgttgggg aaagagtatg
    17881 agatcctgct gattgatgac ggcagtagcg ataattccgc gcatatactg gtcgaagcct
    17941 cacaagcgga gaacagccat attgtgtcta ttttgcttaa ccgcaattac gggcaacatt
    18001 cagcgattat ggcgggattc agtcacgtta ctggcgactt aattattacc cttgatgccg
    18061 atctccagaa tccgccagaa gaaatccccc gcctggtggc aaaagccgat gaaggttacg
    18121 acgtggtagg gactgtacgc cagaaccgcc aggacagctg gtttcgcaaa accgcttcga
    18181 agatgattaa ccggcttatt cagcgcacca ccggcaaagc gatgggtgac tacggttgta
    18241 tgttgcgcgc ctatcgccgt catattgtcg atgcgatgtt gcactgccat gaacgcagca
    18301 cctttatccc gattctggcg aatatcttcg cccgccgtgc cattgaaatt ccagtgcatc
    18361 atgccgagcg tgagtatggt gaatccaaat acagctttat gcgcctgatt aatttgatgt
    18421 acgacctggt gacctgcctt accacaacgc cgctacgtat gctgagtctg ctcggcagca
    18481 ttattgcgat tggaggtttt agcattgcgg tgctgctggt gattttacgc ctgaccttcg
    18541 gaccacaatg ggcggcagaa ggtgtcttta tgctatttgc cgtgctgttt acttttattg
    18601 gcgctcagtt tatcggcatg ggattgctcg gtgaatatat cggcaggatc tacaccgatg
    18661 tccgcgcccg cccccgctat tttgttcagc aagttatccg tccatccagc aaggaaaatg
    18721 aataatgaaa accgtcgttt ttgcctacca cgatatggga tgcctcggta ttgaagccct
    18781 gctggctgcc ggttacgaaa ttagcgccat ttttacccat accgataatc ccggtgaaaa
    18841 agccttttat ggttcggtgg ctcgtctggc ggcggaaaga ggcattccgg tttatgcgcc
    18901 ggataacgtt aatcatccgc tgtgggtgga acgcattgcc caactctcac cagaggtgat
    18961 tttctctttt tattatcgcc atcttatttg cgacgagatt ttgcagctcg ctcccgcagg
    19021 tgcatttaat ctgcacggtt cgctgttacc aaaatatcgt ggtcgcgcgc cgctgaactg
    19081 ggtgctggtg aacggtgaaa cagaaactgg cgttacattg caccgaatgg taaaacgtgc
    19141 cgatgccggg gccattgtgg cgcaactgcg cgttgccatt gcgccagacg atatcgctat
    19201 tacgctgcat cataaattgt gccatgccgc gcgccagcta ctggaacaga cattacccgc
    19261 cattaaacac ggtaatattc tggaaatcgc ccagcgcgaa aacgaagcca cctgttttgg
    19321 tcgcagaacg ccggatgaca gtttcctcga atggcataag ccggcatccg tactgcacaa
    19381 catggtacgt gccgttgccg atccgtggcc gggtgccttc agctatgttg gcaatcagaa
    19441 attcaccgtc tggtcgtcgc gtgttcatcc tcatgccagc aaagcacagc cggggagcgt
    19501 gatttctgtt gcgccactgc tgattgcctg tggcgatggc gcgctggaaa tcgtcaccgg
    19561 acaggcgggc gacggcatta ctatgcaggg ctcgcaatta gcgcagacgc tgggcctggt
    19621 gcaaggttca cgcttgaata gccagcctgt ctgcaccgcc cgacgccgta cccgggtact
    19681 catcctcggg gtgaatggct ttattggcaa ccatctgaca gaacgcctgc tgcgcgaaga
    19741 tcattatgaa gtttacggtc tggatattgg cagcgatgcg ataagccgtt ttctgaatca
    19801 tccgcatttt cactttgtcg aaggcgatat cagtattcat tccgaatgga ttgagtatca
    19861 tgtcaaaaaa tgtgatgtcg tcttgccgct ggtggcgata gccacgccga ttgaatatac
    19921 ccgcaacccg ctgcgcgtat ttgaactcga ttttgaagag aatctgcgca ttatccgcta
    19981 ctgcgtgaag taccgtaagc gaatcatctt cccgtcaact tcagaagttt atgggatgtg
    20041 tagcgataaa tacttcgatg aggaccattc taatttaatc gtcggcccgg tgaataaacc
    20101 acgctggatt tattcggtat caaaacaatt acttgatcgg gtgatctggg cctatggcga
    20161 aaaagagggt ttacagttca ccctcttccg cccgtttaac tggatgggac cgcggctgga
    20221 taaccttaat gcagcgcgaa ttggcagctc ccgcgcgatt acgcaactca ttctcaatct
    20281 ggtagaaggt tcaccgatta agctaattga tggcggaaaa caaaaacgct gctttactga
    20341 tattcgcgat ggtatcgagg cgttataccg cattatcgaa aatgcgggaa atcgctgcga
    20401 cggcgaaatt atcaacattg gcaatcctga gaacgaagcg agcattgagg aactgggcga
    20461 gatgctgctg gcgagcttcg aaaaacatcc gctgcgccat catttcccac cgtttgcggg
    20521 ctttcgcgtt gtcgaaagta gcagctacta cggcaaagga tatcaggacg tagagcatcg
    20581 taaaccgagc atccgcaatg cccaccgctg cctggactgg gagccgaaaa ttgatatgca
    20641 ggaaaccatc gacgaaacgc tggatttctt cctgcgcacc gttgatctta cggataaacc
    20701 atcatgacca aagtaggctt acgcattgat gtcgatacct ttcgtggcac ccgtgaaggc
    20761 gtgccgcgtc tgctggaaat cttgagtaag cataatattc aggccagcat ttttttcagc
    20821 gtcggcccgg acaatatggg ccgtcatctc tggcgactgg tgaagccaca gtttttgtgg
    20881 aagatgctgc gctcaaacgc ggcatcgctt tatggctggg atattttact ggcaggtacg
    20941 gcctggccag gtaaagagat tggtcatgcc aatgccgata tcattcgtga agcggctaaa
    21001 catcacgaag tcggcctgca cgcctgggat caccatgcct ggcaagcccg tagcggtaac
    21061 tgggatcggc aaacaatgat cgacgatatt gcacgcggcc ttcgcactct ggaagagatt
    21121 atcggtcaac cggtaacctg ttctgccgct gcgggctggc gtgccgacca gcaggtgatc
    21181 gaagcaaaag aagcgttcca tttgcgctac aacagcgatt gtcgtggggc catgccgttc
    21241 cgtccactgc tcgaatcagg aaaccctggc actgcgcaaa ttccggtgac cttacccacc
    21301 tgggatgaag tgattggtcg ggatgtgaaa gcagaagatt ttaacggttg gttactcaac
    21361 cgcatcctgc gagataaagg cacgccggtt tataccattc atgcagaagt tgaaggctgc
    21421 gcttatcagc ataattttgt ggatctcctc aaacgcgcag ctcaggaagg cgtgacattt
    21481 tgccctttaa gcgaactgtt atcagagacg ttgccgctcg gacaagttgt tcgcggaaat
    21541 attgccggac gtgaaggctg gctgggttgc caacaaattg cgggtagtcg ctgatgaaat
    21601 cggtacgtta ccttatcggc ctcttcgcgt ttattgcctg ctattacctg ttaccgatca
    21661 gcacgcgtct gctatggcaa cccgatgaaa cgcgttatgc ggaaatcagt cgagaaatgc
    21721 tggcatccgg cgactggatt gtgccccatc tgttagggct acgttatttc gaaaaaccca
    21781 ttgccggata ctggattaac agcattgggc aatggctatt tggcgcgaat aactttggtg
    21841 tgcgggcagg cgttatcttt gcgaccctgt taactgccgc gctggtgacc tggtttactc
    21901 tgcgcttatg gcgcgataaa cgtctggctc tactcgccac agtaatttat ctctcattgt
    21961 ttattgtcta tgccatcggc acttatgccg tgctcgatcc gtttattgcc ttctggctgg
    22021 tggcgggaat gtgcagcttc tggctggcaa tgcaggcaca gacgtggaaa ggcaaaagcg
    22081 caggattttt actgctggga atcacctgcg gcatgggggt gatgaccaaa ggttttctcg
    22141 cccttgccgt gccggtatta agcgtgctgc catgggtagc gacgcaaaaa cgctggaaag
    22201 atctctttat ttacggctgg ctggcggtta tcagttgcgt actgacggtt ctcccctggg
    22261 gactggcgat agcgcagcgg gagcctgact tctggcacta ttttttctgg gttgagcata
    22321 ttcaacgctt tgcactggat gatgcccaac atagagctcc gttctggtac tacgtgccgg
    22381 tcatcattgc cggtagcctg ccgtggctgg gattactccc cggtgcacta tacacaggct
    22441 ggaaaaaccg caagcattcc gcaaccgtct atttgttgag ctggacgata atgccgctgc
    22501 tgtttttctc cgttgctaag ggtaaattgc ccacctatat tctttcttgc tttgcacctt
    22561 tggcaatgct gatggcgcat tacgctttgc tggcagcaaa aaataatcct ctggcgctgc
    22621 ggattaatgg ctggattaac atcgcttttg gcgtcactgg cattattgcc acatttgtgg
    22681 tctccccctg gggaccaatg aacacgccgg tgtggcaaac cttcgagagc tataaagtct
    22741 tttgtgcctg gtcgattttt tcgctatggg catttttcgg ctggtacacc ttaacaaacg
    22801 tcgaaaagac ttggtctttt gccgcgcttt gcccgctggg gctggcgttg ctggtaggat
    22861 tttcaattcc tgacagagtc atggaaggaa aacatccgca attttttgtc gagatgacac
    22921 aagaatcact tcagccaagc cgctatattc ttactgatag cgtcggtgtt gccgcaggtc
    22981 tggcatggag cctgcaacgc gatgacatca tcatgtatcg ccagacaggt gagttgaaat
    23041 acggccttaa ttatccggat gcgaaaggga gatttgtcag cggtgatgag ttcgcaaact
    23101 ggcttaatca acatcgtcag gaggggatta ttacactcgt gctttcggtt gaccgcgatg
    23161 aagatatcaa cagtctcgcc attccgcccg cagatgccat cgatcgtcag gagcgtctgg
    23221 tgctgattca gtatcgtccc aaatgatctg gctaacatta gtctttgcca gcttgcttag
    23281 cgttgccggg cagttgtgtc agaaacaggc aacctgcttt gtggcgataa acaaacggcg
    23341 caaacatatc gtgctgtggc tgggactggc gctggcttgt cttggtcttg ccatggtgct
    23401 ctggctgctg gtcttgcaga acgtaccggt aggcattgcc tacccgatgt taagtctgaa
    23461 ttttgtctgg gtgacgctgg ctgcagtaaa actgtggcac gaaccggtat cgccgcgtca
    23521 ctggtgtggg gtggcgttca ttattggtgg cattgtgatc ctcgggagta cggtgtaatg
    23581 ggcctgatgt ggggattatt cagcgtcatt attgcctcag ttgcgcaatt aagcctgggt
    23641 tttgcggcga gtcatctgcc gccgatgacg cacttatggg attttatcgc ggctctgtta
    23701 gcttttgggc tggatgcccg gatactgtta ctcgggctgc tgggttatct gctttccgtt
    23761 ttttgttggt ataaaacgct acataaactt gcgctaagca aagcctatgc cttgctcagt
    23821 atgagttatg tgctggtatg gattgcttca atggtacttc ccggctggga agggactttt
    23881 tcgctgaaag cactactggg agtagcctgt attatgagcg ggttgatgct gattttcctg
    23941 cccacgacaa aacaacgtta ctgagttttc cctgccactt tacactgccg ttcccactca
    24001 tcggggctat aacagcttgc agagaggact ttcaccgtat gcagcttttc acgattaata
    24061 caataaagcg cattctgcaa aggcgagagt aaatccccca ctttcacggc gaagtcactc
    24121 ttcacttcgg cgatcatttt tatcgcgccg ccagcatcgc agagcataag tacatgtctg
    24181 ttatcttgtt tattgcagca cgattttttg accagccatt ccattgtatt atcctgtttg
    24241 ctaagagttt tcatatcaat ttacagatta acagataaac tctttatcaa cggcaatgga
    24301 atggcagaac tgagaagtga aacggagggt taattattgc tgacgttgca cccactcttt
    24361 tagcgcctga cgtgaaattt taataccgcc gtttttcagc tccggcggca aagtcagcca
    24421 acgtaccggt tgctgaaaac gggctagctt atctttcacc cattcactaa gatcaacgct
    24481 ctcgtggtca tactccatca ccgccaccgg tcgatgacca aactccttgt cggcaacggg
    24541 gacgataaac acctgcaaaa ccgcaggatg tgcagcaatt acgcgctcga cttcttccgg
    24601 ctgaataccc tctccgccac tgaagaatag attgtctaaa cgtccgacaa tggtcagctt
    24661 gccattatgc atctcaccgc gatcgcgcgt agcgtaccag ccttcgtcat taaccagtga
    24721 aaccagttgc ccgttacgcc agtaaccttc tgccatactg gcagcccgca gccacacttc
    24781 attattaacg attttcactt cccgacccgg cagcggcgaa ccaacgtctg ccaggccgtc
    24841 ggcttctttc gcacacaccg tggaggcaaa ctcggtcaga ccatagccgc aaaagcaacg
    24901 aatcccctgc tcgcgcgcct gttccgtcaa ctcgaccggg atagccgcac cgccaagtaa
    24961 caccgctttc agggaaacgg aactgcggtt aaccagcaaa cgccagagtt gtgttggcac
    25021 cagtgaagcg tgagtacagc ctgccagcat ttgctccaat ggctgtttat cacgcaccgt
    25081 catccgcgca ccagcgtata accagcgcca cataattccc tgaccggaga cgtgaaacag
    25141 cggtaaagag agcaaccagt catcgtgatc gccaaacgga atcaacgata acaccccttc
    25201 ggcactggca agatgggcct gataagtatg gacagcggct ttcggcaacc cggtagaacc
    25261 cgaggtcaac gtcattgagc acagacgcgt cggctgccac gcagcggcat gtgcgccttc
    25321 aaccagctga atgtgcagcg acgttaacgc cggaaacgtg ttttccccat ccggcaccag
    25381 cgcaaattgc agcgtcagat tgggcagcaa ttcttcaagc aacggttgcg gcagctgagg
    25441 gttcacgggc aacacccgcg ccccgcattg cagtaacgcc agccaggcga gcagcgtttg
    25501 cggcgtattc cacgcccgca acatcacgcc gctgccctca accacaccct gcaccgcaaa
    25561 tccggaggct aattcatcga cgcgagcaca aagctcgcgc cagttgagtt gctcgtcatt
    25621 aagacgtaag gcgatggctt ctccccgcac ttgccgccag tgacgccacg gccagtcaga
    25681 gaagatcata acaaccgctc cagtgcatca acttccacga caggcagcgt gctacccggc
    25741 cagcgacgta cctgctgcgc ctgcatcaga tccagcgtgt ccagccctgg aatggtgtcc
    25801 ggcgttaacc aggcggcaat ccgcgccagt tgcgttaagc ctaagctcga ttcaatggaa
    25861 gaactgatca ccgccgtcag ccccagcgcg tgcgccgcct gtacctgctc gcgtactttt
    25921 tccagactgc ccgtgagcgt gggtttgata actaccgcgc gcacgccctc ttcagccaca
    25981 aaggcaaaat ccggcccgcg caggctttca tcccaggcaa tggcaatgcc ggtttcacgg
    26041 gcaaacgctc gcgaatcatc gcgggttttg cacggctctt cgagaaacgc gatgcggtcg
    26101 cgataatccg ggttaacgta tttggcaaac tgctgacctt tcagcggtgt ccaggcgcga
    26161 tttgcgtcaa gacgcaaatg cagatccgga attgcctcca gcaacagatt caccaccatg
    26221 ccgtcgcgca ccgcttcgta caatcccact ttgaccttcg ccactttctc gcccggcata
    26281 tcggcaagtt tgaggatcag atcgtccgga tcgccattac acagcggtgc cgcacggtag
    26341 ttggctgctt gcggcaacgt atctgtcagt tctgccaatg cacagcttac gccaaaggcc
    26401 acggaaggca tctgcggtag ctcgcaatcg cctgccagcc agttatttac ccaggcaagc
    26461 agcacacttt gcgcctcttc ccaggtttcc tgactgaagc ccggcagtgg ggagatctcc
    26521 ccccaccctt cgcgctcgcc ttcacgcagg caaacataca gcccgtcgcg ggtttttaac
    26581 cgcctgtcgc gcagaaccac ccccgcgtcc atggggatct gccagcggta tacctgcgcg
    26641 ctacgcatta cggattccgt ttgaatttgc tgaagtcagg ctgacgtttc tggttgaagg
    26701 cgttgcgacc ttcctgacct tcttccgtca tgtagaacag catggtggcg ttgcccgcca
    26761 gctcctgcag ccccgcctgc ccgtcacagt cggcgttcag cgcagctttc aggcagcgca
    26821 gcgccatcgg gctgttttgc agcatttcgc ggcaccaacg gacggtttct ttttccagat
    26881 ccgccagcgg taccacggtg ttgacaaggc ccatatccag cgcctgtttt gcgtcgtact
    26941 gacggcacag gaaccagatt tcacgcgctt ttttctgccc gacgatacga gccatgtagg
    27001 aagcgcccca gccgccgtcg aaggaaccga ctttcgggcc agtctgaccg aagatggcat
    27061 tatctgccgc gatagtcagg tcgcacatca tgtgcagaac gtgaccaccg ccgatggagt
    27121 agccagccac catcgcgaca accggtttcg gacaggtacg gatctgacgc tggaagtcca
    27181 gcacgttcag gtgatgtacg ccggaatcat ctttatagcc gccgtaatca ccacgcactt
    27241 tctggtcacc accggagcag aacgctttat cgcctgcgcc agtcagaatg atcacgccga
    27301 cgttatcgtc ataacgcgca tctgccagtg cctggatcat ctcttttacc gtcagaggac
    27361 ggaaggcatt gcgcacctgc ggacgattaa tggtgatttt tgcgataccg tcggtggatt
    27421 tttcataacg aatgtcctcg aaaccttcgg agcagtcgtg ccattcaacc ggtgcgtaaa
    27481 gcattgcttc atcaggataa atcatattgt gtcctttagt cagaaacgca agatctgcgc
    27541 cagacttgcg attacgccag cgggattttc ccgatgcgcg ttatgtccgg cgcgaggaat
    27601 gacatggcag tcggcagcca gttccgccgc cagggcgcgg aatttgctgt cacgttcacc
    27661 acataaataa taaaacgcaa atgtgcgggc gctaaggtta gcacgtaaat caggctggac
    27721 ggcgagagaa gtcgcctcca gcatggcggc aagcgttgcg ccattattgt tgctgcgcag
    27781 cgccaccagc tcccggcgtt gatcgtcatt gagcgaggca aaaacaggct gttgatacca
    27841 gtcggcaaat accgcggtta atggttcgga acgaaaacgc tgcgcccatt ggcgatcgga
    27901 acgctgacgt tccgcgcgtt gttcagcatt ttgcagcccc ggatgaccgc cttcgacaac
    27961 aaccccacaa agccccgcca gcctctggca agccgccatc atcgccaccc gtccgccaag
    28021 cgagtacccc accagccaga agttaaggat gttgtaacta accagggttt tacgcagtaa
    28081 gtcggtgaca tcatcaaatc catcgacgct aatcgccgcc gaaccaccgt gacctgggag
    28141 atcaacatac aaccgtgagt agtcggcaaa cacctcgccc acttcttgcc attcgtggca
    28201 atcgccggaa aaaccgtgca aaaacaccag ccagggtaaa cctggtttgc cgtgttttgc
    28261 ctgcgcgtgc aggatcataa atggcttacc tgcgccagaa gttgctggag cgtttgcgcg
    28321 ccgtcggtgt cgttaaccac catttcaatt accgtggtgg ttggcgtgcg ccaggcatcg
    28381 gcaagtgccg tttcaagttc ctgccagttt tgcggacgat gatatttcag ctcgaacatc
    28441 gcggcggcgt gctcaaaatg gacgttttgc ggcatcagat agaaacgctc gcgctcgctt
    28501 ttcggcgttg gcagcagcga gaaaatttgc ccgccgttgt tgttcaccac aattaatacc
    28561 agcggcgcgg aaacctggcg caataacgcc agcgcattca ggtcgtaaag tgcggagaga
    28621 tcgcccacaa tcgccagcgt cggtttgccg cttgcccgct gaacgccggc ggcagtcgag
    28681 agcagcccgt cgataccgct ggccccacgg ttgctgtaca ccgggtaacc tgccggaagt
    28741 tgcgaaagcg catcaatcag acgtaccacc aggctgttac cgacaaacaa ttgcccctgt
    28801 tcaggcagat agtcgcagac gcgatgcgcc agttgcgctt cgccaaacgc atcgcgacgg
    28861 gcaataaccg cctgcatcgc ctgctccgcc aggcgcggga tttcaacgca ccagggctgg
    28921 cgtttttctg ccggatgcag ttccagccag tcggcaatat tggcaattaa ccgacgcccg
    28981 cgatggtgcg ccggatcaag ccgcccttcg atgtcatcca caatccagta ctcttcgggt
    29041 tcacagcttg cctgccattg caggagccgt ttgccagtca ggctgcttcc cagttgcacc
    29101 acaatttgcg cctgttgcag ttcgctggtc gctttggcat tgcctaacca gagatcggca
    29161 cacggcagcg gctgtccggt ttgcgacagc acgtcgccaa tcagcggcca gccaagagtt
    29221 tgcgcccaca gtgcaacttt tttgccctct tccgcactca tgcgcccggc aaccaccacg
    29281 ccgcgttttt gccgccagaa gaaccagtcg cgctgttttt cactttccag acgaggcgct
    29341 tcacgcagcc acggcttatc gtcctgccac cagtcgccca gacgctgttg ccagctaagc
    29401 ccggtatcgt ccatttcgcc atacagcggt tcagcaaacg ggcagttgat atggactcca
    29461 cccgcatgaa gcgtaccgag agcgtggtcg atggtagaaa ccagccaacg tgcggggata
    29521 tcctgggtcg ggcgcggcaa tgaaatactg tgcgtggggt gagaggcgaa cattcccggc
    29581 tggcgaattg cctgattcgc gccgcagtca attagctccg gcgggcgatc ggcggttaag
    29641 agaatcagtt tttctccggt taacccggct tcaatcagtg ccggatagag atttgccacc
    29701 gccgtaccgg aggtgacaat caccgccacc ggctgcttgc tgactttcgc cagccccagc
    29761 gccagatgcc ccagtccacg ctcatcgaaa tgggtgtggt gaatgaatgc ggaattctcc
    29821 gccgccgcta acgttaacgg tgtagaacgc gagccagggg cgatacagat gtgtcttacg
    29881 ccgtgacgcg ttaatgcttc cagaatgacc gccgcccagc gtcggttaaa tgcgcttact
    29941 gacatgagtt tgtccggtat caatattgcg gctaagtata aggagctaca aaaatagatt
    30001 attgatatga atcggtaatg atgcgactca ttactattcc atttgtaata aagtacgcag
    30061 ccctgccgct ttgttgtcga tttcctgcca ctcttgctcg gggtcggagc cacggacaat
    30121 gcccgcgcca gcatatagcc gcacgacatt gccgctgatt tttgctgagc gcagggaaac
    30181 acagaattcg ctttgctgta atgagagata gcccgcagaa ccggcgtacc attctcgggt
    30241 gaacggttcg tgacgggcga taaactgtcg cgccagatcg cgcggtaagc cagcaactgc
    30301 tgccgttggc tgcaactgat gtaaacagat cacatcatcc gctttgttga gtgaagtcca
    30361 gatacagcgg cgaagatgct gcactttacg cagacgcagt acctgcggcg gtaaaacatc
    30421 cagcgtctgg gtatcggcct gtaatcgttg gcagatgtct tccaccacca gcatgttctc
    30481 gcgctggttt ttatcatccg ccatcagcca ctctcctaac tgctgcgcct gcttatcatc
    30541 aggatgattt gctactgttc ccgccagcgc ttcagtacgc agcgctttgt cacgccgccg
    30601 ccataaccgt tccggtgaag agccaagaaa agcattttcg ccatcaaagg ccatgtaaaa
    30661 atggtagcaa ttgagattca gtcgacgact ggccgccatc atcgccgccg cattgaccgg
    30721 acttgcgaaa tgcaggtcag ttgcccgagc aaacaccact ttgtcgagct caccttcggc
    30781 gatggttttc gttgccagtt cgattaattg cgtccagccc gttttgtccg gccagtgttg
    30841 ttctcgcgta gtggttaaat gtaacccagg caagggcttg atactcacca gtgtggcgat
    30901 aaattctctt gccttaatcg catcgtgctg aagggagctt tcgctgaata gcgtcagccg
    30961 cagcgtggct ttaccgccac agcgtcgcca ttccaggcgt ggtaaaagta aattgccctg
    31021 cgacgggtca aacgcattca gcccccaaat gcgtaagtcg gcgtgttccg ggtgctggcg
    31081 aagaaaacgt tgtgcctggt ccaacgacgt aaaacgggta atcgcgccca ggacggcagc
    31141 ttcttcatca ccattacgtt gttgccagta gaattgcggg tacgtttgct gactggccag
    31201 ccagctcaag gcatcaaaag cgtctttgag agggaaagga atatcgataa cccgaatgcc
    31261 gggtgtcgcc ggaatctctt gcgacaaatg gcgcagtaga ttttccagcg ccgtagtaag
    31321 tgattgcacg cggacctctc cctgttaaaa gcctcacatt atacggggta ctacaaaaaa
    31381 aatgcagtac cccggtgtag ggaggtttta acggcgtgcc agcaacagtc ctagtaccag
    31441 cccaacggcc gcacccacac caattccctg ccagggtttt tcgtggacgt agtcatcagc
    31501 acgataaaca gcctgcttcg cccgatagta ataactgtct gaagcctggc taacccgttt
    31561 tttcacatca tccagcgctt tttctgcacg cgctttcagc tcaacatatt tctgatcggc
    31621 gggatcgcca gaggagcgga gcacctcttc cagtgtttca ctaagcagcg tcaggtcgtc
    31681 atcgatacgt gtatcaccaa actgattaga cattctcgtt ctccatttgc gtaaaacctg
    31741 tcactaacta tagacaaggg ttattgatta cgcctgaatt acctcgcgtg ccatcccaat
    31801 gtgtgggata ccatcttctt catagatctc cgtcacaggg ataaaaccga aactctggta
    31861 gaagttttgc agatgcgcct gcgccccgag gtagacaggc ttatcaggcc agtgatgcgt
    31921 gcaagtgtcg agggttttac tcatcagttg ctgccccact ttttcgccac gcaacgcttc
    31981 actgacaatc acccgaccta taacgaccgg ctcaagatca tcatcacttt tcagaatcct
    32041 cgcatacgcc accagttcat catttttcca accgagaata tggcgattat cccctgtcaa
    32101 atcatcacca tcgatatcct gataagggca attctgctcg acaacaaaca ccgcgcaacg
    32161 cagttgtaat aaggcataaa gctgggaaac cgataattca gagtgatgca gatcttgcca
    32221 ttcaatcata atgctctcct tttagatgta tgccttatac tagccggaat attttttgaa
    32281 acgtgatgaa ctcatggaat taattttttt aggtacttcg gccggtgtgc caacccgtac
    32341 gcgcaatgtc acggcaatat tgcttaacct gcaacatccg acccagagcg gtctttggtt
    32401 gtttgactgc ggtgaaggca cccagcatca gctactgcat accgccttta accctggaaa
    32461 actggacaag atttttatca gtcaccttca tggcgatcat ctctttggtt tacccggctt
    32521 gctgtgcagt cgttctatgt caggcattat ccaaccctta acgatttatg gtccgcaagg
    32581 tatccgtgaa tttgtggaga ccgcgctgcg gattagcggc tcatggaccg attatccgct
    32641 ggaaattgtc gaaattggcg ctggcgaaat tctcgatgat ggcctgcgca aagtgaccgc
    32701 ttatccaatg gaacacccac tggaatgtta tggctatcgt attgaagaac atgataaacc
    32761 gggtgcatta aatgcccagg cattaaaagc tgctggcgta ccgcctggcc cactatttca
    32821 ggaattaaaa gcgggcaaaa caatcatgct ggaagatgga aggcagatta acggcgcaga
    32881 ttacttagcc gctccagtgc caggtaaagc gctcgctatt ttcggcgata ccggcccctg
    32941 cgatgccgca cttgacctgg ctaaaggtgt cgatgtcatg gtgcacgaag cgacgctgga
    33001 tataaccatg gaagccaaag ccaatagtcg cggtcatagc tctacacgcc aggctgcgac
    33061 actggcccgt gaggctggag tcggtaagct aatcattacc cacgtcagct cgcgctatga
    33121 tgataaaggt tgtcagcacc tgttacgtga atgcaggtca attttcccgg cgactgaact
    33181 ggcgaatgat ttcaccgtgt ttaacgttta actctgctta ttattaaaaa cagggcgaaa
    33241 cttgccctgt tatcgcaacc cgcgctacat atatccaccc acacgggcat attgagcgat
    33301 ttttgatatt tattcatctc cttacgcact atattcagag caataagttg cctcttttta
    33361 tcttcattca gcactacctt tgcaactaaa aggagcatat gatggttaca gttgtcagca
    33421 attattgtca attatctcaa acccaactca gtcagacatt tgcagaaaaa tttactgtga
    33481 ccgaggaatt actacagtct ttaaaaaaaa cagcgttatc cggagatgaa gaaagtattg
    33541 agttactgca taacattgcg ttaggttatg atgaatttgg aaaaaaagct gaagatattc
    33601 tttaccatat tgttagaaac ccaacaaatg ataccctatc gattatcaag cttataaaaa
    33661 atgcctgttt aaaattatat aatttggcac ataccgcaac caaacacccc ctcaattcac
    33721 atgattcaga taatctcctg ttcaaaaaac tattctctcc ttcgaaatta atgacaatta
    33781 tcggtgagga cattcctctc atatcagaaa aacagtcact ttcaaaggta cttttaaatg
    33841 ataagaataa tgaactgagc gatgggacaa acttctggga taaaaatcgt caattaacca
    33901 cagatgaaat atcttgctat cttaaaaaga tcgccgccaa tgcaaaaaat actcaagtca
    33961 attatcctac tgatttctac ctccccaatt ccaacagcac ttacctggaa gtcgctctca
    34021 atgataatat taagagcgat ccatcatggc cgaaagaagt ccagttattc cccataaata
    34081 ctggcggaca ctggatatta gtttcgctac agaaaatagt taatgaaaaa aacaacacac
    34141 aacaaataaa atgcatcata ttcaattcat tacgttcact aggccatgaa aaagaaaatt
    34201 cacttaagcg tatcattaac agtttcaata gtttcaattg tgaccccacg agagaaacgc
    34261 cgaataataa gaatataaca gatcatttaa ctgaaccaga gataatattt ttacatgccg
    34321 atcttcagca atacttaagc caaagttgcg gtgcatttgt gtgcatggca gcccaggaag
    34381 tgattgaaca aatggaaagc aattctgaca gcgcccccta tacgttatta aaaaactatg
    34441 ctgacagatt taaaaaatat tcagcagaag agcagtacga aattgatttt caacatcgac
    34501 tggtaaacag aaattgttat ttagataaat atggcgatgc aaatatcaat cattattata
    34561 gaaacttaga aataaaacac tcacaaccca aaaatagagc atcaagcaaa agagtaagtt
    34621 aacatcattg ttaattaatt cgaccagtca gcaaatatgc tgactggtcg aacatagttt
    34681 gctaaaaagt catcactgac tgatgtcagt cacaccatcc gccagttcaa tcaggcgaat
    34741 aaattccgcc ctgtaaccct gtggatctac cccttttgcc tgctgagccc actgtttgat
    34801 ctgctgccag gaggtattgt tcaggtattc agaaccgcgt aacttttgcc catatgcagc
    34861 tactgctgcg cgaaaacgca tatcttcaga gggcgcgttt attgttggcc ccagcgggaa
    34921 ttcaactaac tgactttctt ttccctgcgg gtatttccag cgaattttta accaggccag
    34981 ttctttcgtt ttgtccgatt tcgctaattt gttatccggg gcatagcgta acttatcaat
    35041 tgatgctttt tgcccgttca gcgttaattc aaataacaac gttatatgtt tgcctgcgcc
    35101 aatatccccc gcatcaacgt tgtcgttatt aaaatcttcc gcccgaagtt ggcgcttttc
    35161 ataaccaatc tgacggtatt ccgttaccca cgcggggtta aactcaattt gcgctttgac
    35221 atcttttgct acggtaatca acgtctgccg catttcactg ttcaatactt tctgcgcttc
    35281 agagagggta tcaatgtagc tgtagttacc gttgccaaca tcggcaattc gcaccatcat
    35341 tgcctcgttg taattgtcat cccccacgcc aaacgtcgac agagtaacac cggactcccg
    35401 ctgttttttg agcattaatt caatcgattt tggatcgtca ataccaacgt taaagtcacc
    35461 gtcagtggcc aataaaatgc gattgatacc gcccttaata aaccccttcg ccgcctgctg
    35521 ataagccagt tccagcccgg caccgccatt ggtactgcct tcggcatcca gcgaatcaat
    35581 tgcggcatta atttccgcct tatgactccc ggagatagaa ggcaatgcaa tacgggagtc
    35641 gccagcgtag gtcacgatgg caatgttatc ctgctcacga agttctttga ccaataattt
    35701 caacgaagac tggataagtg gcaaacgttc atcagaaatc attgaaccag aagtgtcgat
    35761 aagaaagacc agattagaag ctggtaactc ttcacttttg cgatctttcg ccaggatatc
    35821 aactttcagc aatgttcgct gttcattcca tggtgcaggt gccaattcgt agcgcatagc
    35881 gaaaggtatt ggcttagagg ccggaataga ttgtttgtct ttgatatccc aatcagacgg
    35941 gaaataattg actatctcct ccacccgcac agcgtctggc ggaggtaaca gcccttgatt
    36001 gaggaaacgc cttacattcg cataactgcc agtgtcaacg tcaagactaa acgtcgccaa
    36061 cggattttgc gctacctgct taaccggatt atcatcgaac tgctggtagc gagcggttcc
    36121 tggatttgcg atatgtgtag cttttgcttt agccgctctt gcaaatgttg gcgcttcctg
    36181 caatcgcccc tgtaaagcct gtttgtctga atattgttgc acttcttgct gggccaaggc
    36241 tttcgcggcg gcggcgcttt gctcagcctc ttttattgca gcttgctgcg cggcaagcac
    36301 ttgctgctct gtgggagtac tgggttgttg ttgctgactt tccttattct caggttgcgg
    36361 cccacatcct gacaaaatca aactactcat aagcaacatg attatatttt tatttcgcat
    36421 ataaaatcct tttaaataaa ctcaggcacc agacataaaa tatactcttt gtataatcct
    36481 gctaatctta ataaaataca atatcgaaac ccatagcaaa taatgattca acaacgcaac
    36541 ccattaataa tttgcatata aaaaaataat ctttgctttt attatattat ttgtgttttt
    36601 actccctatg attatttttt accaaccctg ggtcaatgca ttaccgtcaa cgccccgaca
    36661 cgcaagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg
    36721 tagtgccgac aatattgata atctgaatag gtccgcagag tatataaaag aggtctttgt
    36781 cagtagcggt gccagagtta cctcgcagga tgtccccatt acgggtggcc cctacaaaaa
    36841 cattgttgct gattatggtc ctgccgatgg accgctgatt attattggtg cgcattatga
    36901 ctctgccagc agttatgaaa acgatcaatt gacctatacg ccgggcgcgg atgataacgc
    36961 cagcggtgtg gcaggattac tcgaactggc acgtttgtta catcagcaag taccgaaaac
    37021 aggcgtgcag ctggtcgcct atgcgtcgga agaaccgcac ttctttcgta gcgatgaaat
    37081 ggggagcgcg gtgcatgcag cttcgcttga gcatccagtg aaattaatga tagcactgga
    37141 gatgattggc tattacgact ctgcgcctgg aagccagaat tacccttacc cggcaatgtc
    37201 ctggctttat cccgatcggg gagactttat tgccgtggtc ggcagaatgc aggatatcaa
    37261 cgccgttcgt caggtaaaag cggcattgtt gtcatctcag gatttatctg tttattctat
    37321 gaatgcccca gggtttattc ccggtattga tttctctgat cacctgaatt attggcaaca
    37381 cgatattccc gccataatga ttactgacac cgctttttat cgtaataaac aataccactt
    37441 gcccggtgat accgcagaca gattgaatta tcagaaaatg gctcaggtag tgaatggtgt
    37501 tataactttg ttatacaaca gtaaataata aaaatttctc gaggtgggtc cagaaaaagc
    37561 gacatgcaca ttttattttc cctcatctgt tttaagatgc gaaacatgaa attcaacgga
    37621 ggaaaaataa atgggaatga ttggctattt tgcagaaatt gactcagaaa agattaacca
    37681 actgcttgag tctactgaaa aacctttgat ggataatata catgacacgc tttcaggatt
    37741 acgccgactg gatattgata aacgctggga ttttttacac tttgggctga caggcacctc
    37801 tgcgttcgat ccagcgaaga atgatccgct gagtcgtgcc gtgcttggtg aacacagcct
    37861 cgaagatggt atagatggtt tcctgggatt gacatggaat caggagttag ccgcgacaat
    37921 cgaccgactg gaatcactgg atcgaaatga actgcgtaaa cagttttcca ttaagcgact
    37981 caatgaaatg gagatttatc cgggagtgac cttttcagaa gagttagagg ggcaactctt
    38041 tgcttcgatc atgcttgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca
    38101 agggaatcat gcgctgacgg ttattgttgg gtgatggtgt cagggcacca aaaaaaaccg
    38161 ccgaacatgt cggcggcttt ctgacttaca aactaacaga ttacatcagt ggcattgcta
    38221 accgcacaat gctaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcgg
    38281 agatcagcac cacgataccg cccgcgctgt actgccagtt tgacggtgca tcgcgacccg
    38341 gttgttccgg tgcgtgcagg tacaggctca ccgccacgcg caggtagtag tagaggccga
    38401 ttgccgaacc gacaaccacg gcacccacca gccaccacaa gtgtgcctgg acaccgaccg
    38461 ccagcacgta gaacttaccg ataaagccca gcgtcatcgg gataccggcc agagacagca
    38521 tcatcaccgt catcaccgcc gcgaggatcg gacgatgcca gaacagaccg cggtaagaga
    38581 acagggaatc agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg
    38641 cgccgaggct gctgaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct
    38701 cgccggtttg cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt
    38761 aaccgagcag acgtttgatg ttggtctggc tcagcgccat caggttaccg aagatgatgg
    38821 aggcaaaggc gataatcgcc agcaccaccc gaatcgcttc gctgtcaccc accggtgcgt
    38881 agaggaacag acgcatcacc acaccgaaga tagcgatttt gctcgccgtc gccaggaaag
    38941 tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca
    39001 gagagagttt gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct
    39061 cgttgagcat accgtcgccg aggtttttac ccagcgcgac aaacgacagg tcgccagact
    39121 gcgcatacac cagcgccata ccgaacagca ggaaagaaga cgctgcggca gaaaggatgg
    39181 tgtatttgat actggcttcc agcgaacgtt tctggcggaa agcgtaaccg accaggccaa
    39241 acagcggcaa agagatcagt tcaataccga ggaacagaga cgccagatgg tttgcattcg
    39301 ccagcaggat cccgcccagc gcggcaatta acaccaacag gtagaactca tccttgttgt
    39361 cgttatagcc ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata
    39421 ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag
    39481 cgcccgcctg gccaacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga
    39541 gcgtagcgtt gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg
    39601 tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg
    39661 cgatttacgg ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg
    39721 cggagtgcga ggtatccaga atcggctgcg gatagaagcc cagcagtacc agcagcacca
    39781 ccagcaacag gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa
    39841 tctggctttt cgctttaccg aagtaagcgc gatgtaacat cgccagcgaa taaacagatg
    39901 caaagaccag cccaaaggta gagataacgg taatcaccgg gacaacctgg aagctgccga
    39961 acagaatcat aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg
    40021 caaagaacag cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc
    40081 gcatgtcgcg ggtatggata cgttcataaa gctgaccgca gagaataaac agacccgccg
    40141 ctgacaagcc gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg
    40201 tgtagatagc aatcagtacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt
    40261 tgatatcggt ctgggcgaag gccatccacg caccgtagaa gatgccgata acacccagcc
    40321 acatagcgat tggcgcgaac tctgccgacg catttgggaa cagcggcagg gagaaacgca
    40381 gcaaaccgta agcggcagtt ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg
    40441 gagcctggga gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttgactg
    40501 cgaaggcgat gaagaagccc agcatcaaca gatattccac accactggac attggcgtat
    40561 tcaacagctc ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca
    40621 gcgccaggat ggcgatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg
    40681 ttgccgccgt gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga
    40741 agtacatcgg caccagcatc atttcccaga agaaaaagaa caggaacatg tcgatggcaa
    40801 ggaacacgcc gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat
    40861 atttttcgat ctctttccac gaacagagta ccgccagcac accgagcaga ccggtcagca
    40921 cgaccattag cagcgacagc ccgtcaatgg cgagatggat agagatacca aaacgcggga
    40981 tccacggcat gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat
    41041 aaccgccctg caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg
    41101 cgatccagcg cggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccga
    41161 taaagggaat taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc
    41221 ccgtcaggac cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt
    41281 agtgtcggat gcaccgcgaa ggaggcttat ccgacctacg ttcttatatg cccaggggca
    41341 atcccacaat ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg
    41401 atgctcatgg atgccacata ccagcgcaga taaccgttct cgcttaacag caggccttta
    41461 cctgcaaagc gggaaagaac cgccggaatg ttcatcattg agttcagcgg atcgcgtttc
    41521 agcaaccagg caatacccag gaacggcttg acgaacactt tgtcgtacag ccagtcaaag
    41581 ccccaggcgt tgtaccacca ggtgcccagc agacggcccg gcgcactgtt ggcgatggag
    41641 gtcaccagag tacgtttacc cagccacagc caggctgcca gcagaatgcc gaccaccgcg
    41701 accacgccag aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc
    41761 ggaagcacgc cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc
    41821 agcacaatca gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct
    41881 tttccgtgga agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg
    41941 accagacctg ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca
    42001 tccttactga agaagcccgc agtgaccagc ggcagtgccg acagtgctgc gccgcccacc
    42061 aggaagcaga gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc
    42121 tgttcgtgat ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag
    42181 aacgcgtggg tcatcaagtg aaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg
    42241 aacatgtagc caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt
    42301 accagcgcgg caaaaccggc cagcagcagc gtaaccgccc cgacaatacc caccagatgc
    42361 agaacttccg gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg
    42421 gttaccatcg ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg
    42481 gcaagccatg tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc
    42541 agcgtcgccc acatcagcat attattgccg tcagcaaagt gcgctggtgc cagttccacc
    42601 atttcgcgga agttcagggt gcccagttcg ttgtaaagaa tgaacagcgc gaaagcgagg
    42661 aacacgtcac ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga
    42721 tcggtgtaat agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg
    42781 tacatcagca gcaggttgtc ggcaagcacc agaactacca tgctggcgat gaacaggttg
    42841 gtgtaggcga agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg
    42901 tggatcagga aacccacacc agtgaccacc gagagcatgg tcagcgacag gccgtccagc
    42961 accagattga aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg
    43021 taagcctgct cgccgttagc gaagaaatca acgccgataa aagcggttac cagcgccgcc
    43081 aggcccacag agccaacgcc gacgatcgcc gaaacgtttt cagaccagcg cccacgggag
    43141 aacgccagca ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc
    43201 cgcgcatctc acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca
    43261 gcagcgcaag gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca
    43321 cctgaccgtc ggtctgaccc cagtagcttc cggcgaccac aaaggccagc gcggaggcgt
    43381 taatcatgat ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg
    43441 ttaagccaag aacgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc
    43501 gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc
    43561 acgctcttca cgaccgacgt ggaaggccac aaccagaccc gcgagcagca gcatagaagc
    43621 cagttccacc gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat
    43681 tggcgtaccg tcgatacctt gatcgttaac accgagaatg gcgtaaacaa tcaccaccaa
    43741 catgatggct gacaaaattg ccggaccaat ccacacctgc ggtttcagcc actggcgttc
    43801 ttgttcgatt tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat
    43861 aatggcaccc gcgtagacga taatttccag cgcaccggcg aagtaagcgc ctagtgagaa
    43921 gaacaccccg gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt
    43981 ggtatgggtg atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc
    44041 gaactccatt gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg
    44101 ttctctgctt cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta
    44161 tattccgggt atttgcccgg accggagatc agcagatcct ctttctcgta aaccagatcc
    44221 tggcgcttgt attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag
    44281 gcttcttcgc acaaaccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga
    44341 taccagcgac cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct
    44401 accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg
    44461 atacgaccac gataacgggg cggcagatag accggctctt ccgggtacat tcgcgtttcg
    44521 cgtttggcga acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct
    44581 actaacaatt cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga
    44641 cagccgccgt taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg
    44701 acattacctg gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca
    44761 taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc
    44821 caccgaagaa cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccca
    44881 cgaagaacag accgaacttc atgccggaat attcaatgtg gtaaccatcc gccagttcct
    44941 gctcggcttc cggctggtca aacgggtgac ggtgacacac cgccacgccc gcgatggcaa
    45001 aggtaataaa accaaagaat tgcgggatga cgttccacac atgcgcctgg ctgttgacga
    45061 tgtcggtcat gttgaatgaa ccggcttgcg ccaccacgcc catcagggaa agcccgagga
    45121 acacttcgta gctcagggtc tgcgcagaag cacgcatcgc acccagcagc gagtatttgt
    45181 tgttacttga ccagcccgca aacagcaccg cgtaaaccgc caaaccggcc atcatcagga
    45241 agaacaaaat cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaatcg
    45301 caaaggccag cagcagtgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat
    45361 ccgagaattt cgggatccag tcttctttaa agaacatttt gatcatatcc gcgaccagct
    45421 ggagcgaacc gccccagcca acacggttag gtccgtaacg gttctggaac agacccagca
    45481 ggcgacgttc gccaaagctc atgaatgccc cgcaggtgac aaccaccagc aggatcacca
    45541 ccgctttgag gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg
    45601 tgcctccttg agatcctcaa gatgcgcgcc agccagcacc ggagcaatgc cggacatacc
    45661 catcggcaag cccacctgcc ctgccgtcag cccttcagcg atttcaaccg gcagcgtgac
    45721 cgtgttgcca tcgtaactaa aggagacgcg tgtacctgcg ttcacaccca acttcgcggc
    45781 atccgctggg ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg
    45841 ctgtgacaat tcatcactgc caaacaggtg gtaatacggc gcaatacgcc atttcccgtc
    45901 ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc agaccattct cgctggtttc
    45961 aaacagacgc acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt
    46021 gttccacgcc tgcggggagt tccagcctgg cgcccaggca aacggcactt gcgaacggtg
    46081 cgcggtcggc tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg
    46141 ctgacgcggc tcatgaacgc taatattggc gcgcatggcg gtacgaccgc tgtaacggtg
    46201 cggttcacgg gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt
    46261 gatacctgcc agttccggga ttttcgccac aacagcgtca atcacatggt cgagctgcgt
    46321 ccagtccact tcacggctca acagggtact gtgcagcgag tgtaaccagc gccagctttc
    46381 cagcatgaca gttttgctgt cgtaataagc cggatcgtaa acctggaaga aacgttgggc
    46441 gcgaccttca ttattaatta ccgtaccgtc gctttcagca aagctggcag cggaaagtac
    46501 cagatgggcg ttttccatga tcgctgtgcg ttgatggtca accaccatca ccagcggcgc
    46561 tttagccagc gcagcattca cgcgggtagc agaagcgtga cgatgcagat cgttttccag
    46621 caccaccacc gcgtcggcgc gtccggtttc cagttcggtt aacgcttctt caagcgatcc
    46681 accgcccata atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccgac
    46741 gtcagcgccg cgacctttca gggctttcgc gacgttagcc gcagcctgaa ttacttcagc
    46801 gctaccggcg ttcgtcccgg agataatcaa cggtttcttc gcacccgcca gtgcctgcac
    46861 gatgacgtcg attttgcttt gcagttcagg ttcaataccg tcaaccgctg gcgcggagtt
    46921 atccagcgca tgggcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta
    46981 agtccacgcc gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg
    47041 cttcgcacgt tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc
    47101 tgccgccatt tcgcgcgctt tacctttcac cgcctgacgc actgccagcg cgacgcgcgc
    47161 gccggtctgg gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg
    47221 cagagcagga gtataaatgc cgccttcacg cagtactttc agcgccagtt gcagacgttc
    47281 ctgctcaccg tgagcgatac cggtgtagaa gttttcttcg cccaccagct cacgcagcgc
    47341 aaagttactt tccacgctgg cgcgcggaga accaatgccg atcactttct tcgactgacg
    47401 cagaatatct gctgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc
    47461 acgacgctgt actggctgac gcggacgatc tttcaggttg acgtaaccgt aaccgaaacg
    47521 accacggtcg cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg
    47581 cagttcgcca taacgctcgc cggggctgat gttacagccg atggaacatt gctggcagat
    47641 gctcggcgca aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt
    47701 aaatacgccg gtcgggcaaa tttcgaccag gttaccggaa aattcgcttt ccagcgtgcc
    47761 gtcttccggg cgaccgaagt agacgttgtc gtgcgcgcca taaacgccca gatctgtacc
    47821 gtcagcgtaa tctttgtagt aacgcacgca gcggtagcag gcgatgcagc ggttcatttc
    47881 gtgagagatg aacggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg
    47941 acggaagctg tgtccggtca tcacggtcat atcctgaaga tggcagttac cgccttcttc
    48001 acataccgga cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa
    48061 ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca
    48121 ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg
    48181 gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc
    48241 cagagacaga caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc
    48301 tacatgaatt gtagccatta gcatgcttcc agttttctca gtcagagact gagcgttaat
    48361 cgaaattcgg ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg
    48421 tattgctgaa cggctgtttg atccccgcct caaattcttc gcggaaatat ttgatggcgc
    48481 tctgtaacgg ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga
    48541 atcgacacag ttgctcaagt gtttcgatat cgcccggctg accttcgcca cgctccagcg
    48601 cacgcagaat tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg
    48661 actcacgggc gaaaaactct tccaggttac gcaccagcga caccatgttg atctcatggt
    48721 caaccgccat cgccagcgcc gtacccagac ggcttcccgc tttaccgata ctttcgaatt
    48781 ccatcggcag atcaaggtgc gcttcggtca ggaagtcagt ccccgcgccg cctggctgcc
    48841 aggctttaaa tttcagacca tcacgcatac caccggcgta atcttcgagg atctcgcgtg
    48901 cggtggtgcc gaacggcagt tcccacagtc ccggattttt cacccgaccg gagaagccca
    48961 tcagcttggt gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag
    49021 cgaggatcgc cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttgcccc
    49081 atacgccgga ggttgccggg aaggggggtt tcgagcgtgg gttagcacga cgcccttcca
    49141 gggagttgat taacgctgtt tcttccccgc agatgtagcg ccctgccccg gtatggacga
    49201 acagttcgaa gtcgaaacct gttcccataa tgtttttgcc aagcaagccc gcttcagtgg
    49261 cttcggcaat ggcgcggcgc agatttactg ccgcttcgat atattcgccg cgcaggaaga
    49321 tgtagccacg gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt
    49381 gcggcagttg ctccatcaac aggcggtctt tataggtgcc cggctccatt tcatcggcgt
    49441 tacacagcag gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc
    49501 cagtcgaaaa gcccgcgccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga
    49561 tttcgtccgg agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc
    49621 ggtattcgtc cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg
    49681 tttcgggagt acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc
    49741 cggggtcaga tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt
    49801 ccccaggcag caagttggca gcagcgtaaa gcggccatca aatgtcgttt gccctggttt
    49861 gatgttcagc tttttctcga gcgccgcctg aataccctga tacccgttga tatgacagac
    49921 cacgctgtcg caataacgga tcacatggcg accaaccggc tgacggaaga tctgactgta
    49981 gaacgttgcc acaccttcga cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg
    50041 gatcgcacca tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga
    50101 cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc
    50161 actcagctca aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca
    50221 tctgacataa caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg
    50281 cggatcgccg ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga
    50341 taactcatgg tgctaccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca
    50401 atcatctgga aagattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg
    50461 gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg gcggtggcgt ggtcagcggg
    50521 tgatccgctt tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga
    50581 ctctggcgca gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacca
    50641 ccaaccggga tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg
    50701 tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct
    50761 ttcgcgccat aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc
    50821 gctttctcgt aagacgccag acgtttcggc atccagtcga ggaactcacg cagcaggcga
    50881 tcccagccac gcggcaggtc atgcgctaca ccgccaatac ggaaccacgc cgggtgcata
    50941 cggaaacccg tgattgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag
    51001 aacacgggcg tcattgcgcc gacgtcctga ataaaggtcg agatatacag caggtgactg
    51061 ttgatgcgga acagttcgga gagcattacg cgaatgacgt taacgcgatc cggcacggtg
    51121 atcccggcca gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg
    51181 tattcgatac ggtcggtata cggaatgtag ctgtgccagg actggcgttc gcccattttc
    51241 tccgcaccac ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg
    51301 agctgcaaaa cgatacggaa agcaccgtgc gccgacgggt ggttcggacc gaggttgagg
    51361 aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc
    51421 agggcttcca tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta
    51481 gcgcgcgccg ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg
    51541 cggcgcaggt tcgggtgacc gtcgaatgta atgccaaaca gatcccaggt ttcacgctca
    51601 taccagttag cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc
    51661 agcgccacct tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg
    51721 gaaaaatccg cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt
    51781 aagtcaaaca gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt
    51841 aattgttcac gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag
    51901 gcatccggcc caaaacggtt gcgcagttcg ccaattaccg gatcatcaag atgatcgcgg
    51961 gtctgccagg cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt
    52021 ccgtggtgac tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa
    52081 atccctttgc cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca
    52141 atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga
    52201 tcgccaacca cccaggagag cggacgacgt tctttgccga tagattcctg caacagcatc
    52261 agtgcctgca tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg
    52321 atgaatttat cgacgccctg cacaacggaa taaatatcgt acataccacc agagttggca
    52381 caggcaccca ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata
    52441 accggtgcca ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc
    52501 gaagcacgca atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc
    52561 accatctcaa cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga
    52621 ccccagttaa ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc
    52681 agagggtcgg ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga
    52741 tctatgcggg tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg
    52801 atactgttcg tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc
    52861 gcgccaatac gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct
    52921 tccacaaagc ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc
    52981 gcttcaacgt cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt
    53041 aagcgggcgg agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg
    53101 cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca
    53161 acgataagaa agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata
    53221 ctcattgctt actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc
    53281 cacatcgatt catggggagg aataaaaaaa accttacaat cactgtagaa attcttttat
    53341 acagctaatt gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag
    53401 cttcttcaca ttagtttaca taatatcaac accattagca tttaatgccc tttcacccca
    53461 gatccttgac gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg
    53521 gaaaaaatac gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt
    53581 attttcttga tctgtgacac gcttttgtca ttccataaca aaaacgcagc aacaaattta
    53641 cgtatttttt aacatcattg tagcaggtga tttttttcag gcgattattt gtgcgtacgg
    53701 ggcgtgaatc tctggtgatt gaaaaatgaa cagttttgta cgttctgcac gatgtgaaga
    53761 aggttcttta ctaaaaaaaa gccgctgggg tttaaaacac ccccagcggc tcgtttttta
    53821 cactattgtc tcaggaatta tctatggtcc gtcgattact cgatatccct ttcaatcaac
    53881 aatgaatcat ccccttccgg agcagatatt gggctgtatt gccacggatt atggtagctc
    53941 tccatcgcct gatagatcac ctgggccagc tcattattac tggacgggtc atagcacagc
    54001 aaatattcgg tatcaggtag cggcggtaag ccatctactc cactcagcac acgcaagtcc
    54061 ggactcatca tctcgaccgg ccttgccgtc acgccaagac cggctttcac tgccgcacga
    54121 acggccggaa gcgtcgaggc gacataagcc agtcgccagg gaatatctgc tttattaagc
    54181 gtcgccagca ccatatcgcg aaacggactt ggatcatcca gcaatacaag agggatcggc
    54241 tcaccttttt gcaatacgta ttccgctgcg cagtaccagt gtgttggcga ggtacgcagg
    54301 ttcaatgcct taaacgctga aggacgatgg gtggttacca tcaaatccac ttcctgggat
    54361 tccagcattt ctgccatata ggcattacgt ttaacgcgga catccagcgc taatttcgga
    54421 taaaccgaac tcacgcgatt taacaggaaa ggtaagatcg tatcggcaga ttcatctgaa
    54481 gcaccgatag ttaacacgcc ctgaagatta ctgaacatta atgatgagca ggcctcatca
    54541 ttaaaacgca ggattttcct ggcgtaacca agaagttgaa tgccatgttc agttaacagt
    54601 ttgttgcgac cgtgacgagc gaacagttct ttcccaacga gttgttccag acgctgcatt
    54661 tgctgactta cggcggactg agtacgacac acagcggcag ctgcggcagc aaaagtgttc
    54721 agatcggcaa cagcaacaaa tgttctcagc agatcgaggt cgaggttaat tatcggacga
    54781 tttgcactta tcatatatta tcacttactg gcggctcata ctgagctggt taatgctgtg
    54841 cacacacaaa caagcaattc catttgtaat gtgcctccct ggcagtttca tccggaaccc
    54901 ggacgaaagt aaaaatgcat atgagttgca ctaaaaaagc gactcacatt gttccgttat
    54961 aatgcctgaa gtagatcaca gaatatatct tcagggatcg catatctatt aagttactca
    55021 ctcttttcta tttatgacat gcgcgtgttt gtataaatgt aaatgtgagt ccttgttcca
    55081 ctctcgtgca gcatcgctgg tcatacgcga acacgtacaa acagcaatgg tgaggcggca
    55141 tcaagagcag ggatccgtca tttatgcgag cattttaccc caaaaacctt ttatttataa
    55201 gggtcattgc gaattatctg atgcaaagtt atgttatgtt aggcaaagta atcttctatt
    55261 attaataagc acatcaaaac ctttttgaat attaaataat aattaattag catcatcctc
    55321 attcattaat tccgcttaac aatagtttca caccttgccc ccattgcgac acctcggaaa
    55381 tcatcacgcg gttaaactct ccgtaatgcg gaaatatcat catcgcgttt cattagtgaa
    55441 ttcttaaatg aggcattttc acacaattat cttacagata aaaaaaccag acttacaatt
    55501 aagaatcaga acaattcact atataacatt gcatgtaaag catatacacc tcattatttt
    55561 gtcattatta agttattaac agcacaatcg agccttcccc tctggcaaaa tcttattctg
    55621 cagaccttca aaacaccgtc ctgggggagt acattgttct aagctgactt ccacggcagg
    55681 gagtggcgat aacagcaaaa aaggtcaaga ttcatgtccc ccattgaaaa atccagcaaa
    55741 ttagagaatg tctgttatga catccgtggt ccggtgctga aagaagcaaa acgcctggaa
    55801 gaagaaggta acaaggtact gaaactgaac atcggcaacc cagccccgtt cggttttgac
    55861 gcgccagatg aaatcctcgt tgacgtgata cgcaacctgc ctaccgctca agggtattgc
    55921 gattccaaag gtctttactc cgcgcgtaaa gccatcatgc agcactatca ggctcgtggc
    55981 atgcgtgatg ttaccgtgga agatatttac atcggcaatg gtgtatcgga gcttatcgtt
    56041 caggcaatgc aggcattgct gaacagcggg gacgaaatgt tggttcctgc accagattac
    56101 ccactatgga ccgcggcggt ttcgctttcc agcggtaaag cggtgcatta tctttgcgat
    56161 gaatcctctg actggttccc ggacctcgat gatattcgcg ctaaaattac gcctcgtacg
    56221 cgtgggatcg ttattatcaa cccaaataac ccaaccggcg cggtatattc caaagagctt
    56281 ttaatggaga ttgtggagat tgcacgtcag cataatctca ttatcttcgc cgatgaaatt
    56341 tatgacaaaa ttctctacga cgacgctgag catcactcaa ttgcgccgct ggcacctgac
    56401 ctgctgacca ttacctttaa cggactgtcg aaaacgtacc gcgttgcagg cttccgtcag
    56461 gggtggatgg tgttgaacgg gccgaaaaaa cacgccaaag gctacatcga aggtctggaa
    56521 atgctggctt caatgcgcct gtgtgctaac gttcctgcgc aacacgccat tcagaccgcg
    56581 ctgggtggtt atcagagcat cagtgaattt attacccctg gcggtcgtct ttatgagcag
    56641 cgtaaccgcg cgtgggaatt gatcaacgat attccgggcg tttcctgcgt gaaacctcgt
    56701 ggtgcgctgt acatgttccc gaaaatcgac gccaaacgct ttaacattca cgacgatcag
    56761 aaaatggtgc tggatttcct gttgcaggaa aaagttctgt tggtgcaagg gacggcattc
    56821 aactggccgt ggccggatca cttccgcatt gtcacgctac cgcgtgtcga tgatatcgag
    56881 ctgtctttga gcaagttcgc gcgtttcctt tctggttatc atcagctgta atcttaattt
    56941 cactgccgga gattgcatcc ggcagtgtta tcccgccaca atgacctgat gatgtcatca
    57001 tacgtaaggt cactatgaaa cagagccatt tttttgccca tctctcccgc ctgaaactca
    57061 ttaaccgctg gccgctcatg cgcaacgtgc ggacggaaaa tgtgtccgaa cacagtttgc
    57121 aggtagcgat ggtcgcccat gcgctggcag ctatcaaaaa tcggaaattt ggcggtaatg
    57181 tcaacgccga acgtatcgct ttactggcga tgtaccacga tgcctcagaa gtgctcaccg
    57241 gcgatctccc tactccggtg aaatacttca attcgcaaat cgctcaggag tacaaggcta
    57301 ttgaaaaaat cgctcagcaa aaactggtcg atatggttcc ggaagagctg cgggatatct
    57361 ttgcgccgtt aattgacgag catgcatata gcgatgaaga aaaatcgctg gtgaaacagg
    57421 cagatgcact gtgtgcatat ctgaaatgtc tggaagaact cgcggccgga aataatgaat
    57481 tcttgctggc aaaaacgcga ctggaagcga cgcttgaagc gcgtcgcagc caggagatgg
    57541 actacttcat ggaagtattt gttcccagct tccatctttc gctcgatgag attagccagg
    57601 attcaccgct gtaagcagcg ggagtctgcg tcgcatcagg caataagcgc cggatgcgac
    57661 atcaggctct tgtcaaaacg gaaacagcat cgggatcatc accacacaaa ccgccatcac
    57721 gataatggtg aacggtaccc ccagcttcac aaagtcactg aagctgtaat ttcccggacc
    57781 taaaaccagt gtgttaaccg gtgaagaaac cggtgtcata aaggcggcgg atgctgccat
    57841 cgccacgacc atcgcgaatg gataaggcga cacccccatc gttttggcag cagccagcgc
    57901 aatcggagcc atcaacaccg ccgtcgcggt attagagata aatagcccaa taaccgccga
    57961 caagacaaac aaacaaccca gcatcatatg tggcccgtaa ccaccgccaa tgtccatcag
    58021 ccctttcacc gccagcgcga cacctcccgt tttctgtaat gccacagcaa acggcatcat
    58081 cccaacgatc aaaataatgc tcggccagtg aatggattta taggcgcttt cagcatctat
    58141 acagcggaat ttccccatca gcaggcaggc gatgatagcg gcaacaggat taggaatttc
    58201 atctgtcagc attaacgcca ccattagcac cagacagaaa atggcatggg gtgcctggct
    58261 gtgcgcgggc gatgcttcac tcacctcttc cggtaagttc agcgctacga agtcgcggcc
    58321 ctgtttggcc agcataccga tcagtttcca gttacccaca accaggatga tatcgcccag
    58381 cagcagaggc tcatccgcca gcgaaccttc cagcgccacg ccattgcgct ttagccccac
    58441 cacattcagt ccgtagcggg tacgaaaacc aatttcgcgc accgatttac caatcagttc
    58501 tgactcagga attaatgaaa tctctgccat gcccacatca agggcctggt cagaaaaata
    58561 ctcgccgcgc agtaccatcg gctccagcaa ttgctcacta caaaattgcc ggagatcgac
    58621 atcagccgca gacatatcaa taagcaaaac gtcacgcgcg cgaaattcag aaaccccatt
    58681 aacgttcacg ataacgcgac gaaaacgccg ccagcgttca acaccgatga cgttagcgcc
    58741 ataacgctca cgtaatttga gatcatccag ccgttgacca atcattggcg atccggggcg
    58801 aatagccaga cgtcgcgcac gcccggtcag tcgatattca cggataagat cgcgaaaggt
    58861 tcgacgcgtc cagccttcgc gctgcggggt ctgggtatcc cctttcagca tgaaacgcat
    58921 cactaacata tacaaaatac ccagcaccag cacgaccagg ccaataggtg ttacgctaaa
    58981 gaaactgaag ccgtgatagc cttcacgcag caattcactg ttgactacca ggttcggcgg
    59041 cgtcgccacc agcgtcatca tgccgctaat cagcccggca aaacttaacg gcatcatcag
    59101 acgtgacggc gaagtttgca tacgcatggc aacgcttaac accacgggaa taaagatagc
    59161 gacaacgccg gttgaactca taaacgcgcc aagccccgcg acggtcagca tcaacaaaac
    59221 caacattttg atttcactgt tgcccgcaac tttgaccagc catgttccca ttacggtggc
    59281 aacaccggta cggaccaaac catcgccaat aataaacaag gcggcaatca ggacaacgtt
    59341 aggatcagaa aagccggaaa atacttctgg gactgtcagc gttccgctta atgcaaacgc
    59401 gacaataaca aacaaagcga ccgcatccat acgcactctg cccgtcgcaa acaagacgat
    59461 ggcaacggcc agtaatgaaa gaacccaaat caattcaccg ttcacaactt atccttgtta
    59521 attgaggggg atgacttgat tctgccataa aaaagccccg acgagacggg gctaaatcat
    59581 gatcaggtgt ttcactgaat aataacatcg ccatttggct gtttggtcac agtaatttga
    59641 tccagactgt ggaggaccaa atcgacctca ttcaggcgcg gggtatctgc cggagcgtta
    59701 accgcaatga catggcaacc cgccgccagg ccagaaagca cgccagcggg agcatcttcc
    59761 accaccacac actcctgcgg cgcaagcccc agcagctgtg cgcctaacag atacgcatca
    59821 ggttctggtt ttccgcgctt cactcgctca gcggtcacaa acacctctgg cgcgggaagc
    59881 cccgctattt tatggcgcgc tcgcgctacc ggcatggagc cagaagtcac aatggcccac
    59941 ggaatacctg ctttattcaa atgattgagt aaggcaattg cccccggaag cgcggtaata
    60001 ccttcggttt ccgtggcctc gatttgctcc agacgcgtaa actcggcggc aatatcagcc
    60061 tcggatttgc ccgccataaa atggcgcaga gaggtgatcg cctgtttacc gtgaatgaaa
    60121 gccagcacct cttccggcgc taacccatga cgtctggccc agttgctcca cgcccgttct
    60181 accgcaggca gggaatccac cagcgttcca tcaagatcaa acagaaaacc tttgcaccgc
    60241 acgcgggcct cctcaggcgt tgataatttg attaatttcg ttggcgctca aatggtactg
    60301 acgcgggcag gcatgccaca cattaagcat gcgctgatat ttttcccaca ttggcgtctg
    60361 ggcgttaaaa ccgtgagttc cggcgtcaaa atgggtatag cgcccttcca cattaaccat
    60421 aaagcggaca taaccgaggt aacgtgcttc agtggcggcg tcaaagccga ggaaggtgac
    60481 acgacgttca tcgatggatt gctggtcctg caaattagac caggaaacat gcaacgcatg
    60541 atacatctcc ataatgtcga tgatagtgcg gcaggtttct tctttcagct cgccaaactc
    60601 gcgatccagt tcacgcatct gtaatccata accacgctca atgattgttt gcaggcgacg
    60661 gtaacgttca gcatttgccg gatcgagcat agtcatcatc ttgtactggt tagacaaaat
    60721 aagacgttgc gcgttggtca tttccattgt tgactcctgt atcactctat tacggtgaaa
    60781 aaaaagaagg ctgagtatgc cttcttttat atgcgtaatc aggggtcaat tacaaatcat
    60841 caaggaaagt tttatcctgt tgtttgaagg cgcgcttaag cgtgtcagct aatgcctggt
    60901 aatcaggctt gccttcaacg ggtgccaaca cctgtccaga ctcctgcaat ttaccgcgaa
    60961 cttcataaaa ccagttgagg attgcagggg gtaatggcgt gacagaacgc ttgcccagcc
    61021 accacaatcc ctgcatgggt aaacttaagg cgaacagggc agtggcaact gccggcccaa
    61081 gctgaccgcc cagggcaatc tgccagcaga gagtaaatac ggcgatcggc ggcataaaac
    61141 ggatcgcata acgcgtcatc ttgataacgc gattttcgac aaagaccggg gcaaggcgtt
    61201 tttccagcgg ccacgtcttt gagtaatgct gtccccggcg aaacaagcta aaaaaattaa
    61261 cagaacgatt atccggcgtt gacatgcttc acctcaactt cacatataaa gattcaaaaa
    61321 tttgtgcaaa ttcacaactc agcgggacaa cgttcaaaac attttgtctt ccatacccac
    61381 tatcaggtat cctttagcag cctgaaggcc taagtagtac atattcattg agtcgtcaaa
    61441 ttcatataca ttatgccatt ggctgaaaat tacgcaaaat ggcatagact caagatattt
    61501 cttccatcat gcaaaaaaaa tttgcagtgc atgatgttaa tcataaatgt cggtgtcatc
    61561 atgcgctacg ctctatggct ccctgacgtt tttttagcca cgtatcaatt ataggtactt
    61621 ccatgtcgag taagttagta ctggttctga actgcggtag ttcttcactg aaatttgcca
    61681 tcatcgatgc agtaaatggt gaagagtacc tttctggttt agccgaatgt ttccacctgc
    61741 ccgaagcacg tatcaaatgg aaaatggacg gcaataaaca ggaagcggct ttaggtgcag
    61801 gcgccgctca cagcgaagcg ctcaacttta tcgttaatac tattctggca caaaaaccag
    61861 aactgtctgc gcagctgact gctatcggtc accgtatcgt acacggcggc gaaaagtata
    61921 ccagctccgt agtgatcgat gagtctgtta ttcagggtat caaagatgca gcttcttttg
    61981 caccgctgca caacccggct cacctgatcg gtatcgaaga agctctgaaa tctttcccac
    62041 agctgaaaga caaaaacgtt gctgtatttg acaccgcgtt ccaccagact atgccggaag
    62101 agtcttacct ctacgccctg ccgtacaacc tgtacaaaga gcacggcatc cgtcgttacg
    62161 gcgcgcacgg caccagccac ttctatgtaa cccaggaagc ggcaaaaatg ctgaacaaac
    62221 cggtagaaga actgaacatc atcacctgcc acctgggcaa cggtggttcc gtttctgcta
    62281 tccgcaacgg taaatgcgtt gacacctcta tgggcctgac cccgctggaa ggtctggtca
    62341 tgggtacccg ttctggtgat atcgatccgg cgatcatctt ccacctgcac gacaccctgg
    62401 gcatgagcgt tgacgcaatc aacaaactgc tgaccaaaga ttctggcctg ctgggtctga
    62461 ccgaagtgac cagcgactgc cgctatgttg aagacaacta cgcgacgaaa gaagacgcga
    62521 agcgcgcaat ggacgtttac tgccaccgcc tggcgaaata catcggtgcc tacactgcgc
    62581 tgatggatgg tcgtctggac gctgttgtat tcaccggtgg tatcggtgaa aatgccgcga
    62641 tggttcgtga actgtctctg ggcaaactgg gcgtgctggg ctttgaagtt gatcatgaac
    62701 gcaacctggc tgcacgtttc ggcaaatctg gtttcatcaa caaagaaggt acccgtcctg
    62761 cggtggttat cccaaccaac gaagaactgg ttatcgcgca agacgcgagc cgcctgactg
    62821 cctgatttca caccgccagc tcagctggcg gtgctgtttt gtaacccgcc aaatcggcgg
    62881 taacgaaaga ggataaaccg tgtcccgtat tattatgctg atccctaccg gaaccagcgt
    62941 cggtctgacc agcgtcagcc ttggcgtgat ccgtgcaatg gaacgcaaag gcgttcgtct
    63001 gagcgttttc aaacctatcg ctcagccgcg taccggtggc gatgcgcccg atcagactac
    63061 gactatcgtg cgtgcgaact cttccaccac gacggccgct gaaccgctga aaatgagcta
    63121 cgttgaaggt ctgctttcca gcaatcagaa agatgtgctg atggaagaga tcgtcgcaaa
    63181 ctaccacgct aacaccaaag acgctgaagt cgttctggtt gaaggtctgg tcccgacacg
    63241 taagcaccag tttgcccagt ctctgaacta cgaaatcgct aaaacgctga atgcggaaat
    63301 cgtcttcgtt atgtctcagg gcactgacac cccggaacag ctgaaagagc gtatcgaact
    63361 gacccgcaac agcttcggcg gtgccaaaaa caccaacatc accggcgtta tcgttaacaa
    63421 actgaacgca ccggttgatg aacagggtcg tactcgcccg gatctgtccg agattttcga
    63481 cgactcttcc aaagctaaag taaacaatgt tgatccggcg aagctgcaag aatccagccc
    63541 gctgccggtt ctcggcgctg tgccgtggag ctttgacctg atcgcgactc gtgcgatcga
    63601 tatggctcgc cacctgaatg cgaccatcat caacgaaggc gacatcaata ctcgccgcgt
    63661 taaatccgtc actttctgcg cacgcagcat tccgcacatg ctggagcact tccgtgccgg
    63721 ttctctgctg gtgacttccg cagaccgtcc tgacgtgctg gttgccgctt gcctggctgc
    63781 catgaacggc gtagaaatcg gtgccctgct gctgactggc ggctacgaaa tggacgcgcg
    63841 catttctaaa ctgtgcgaac gtgctttcgc taccggcctg ccggtattta tggtgaacac
    63901 caacacctgg cagacttctc tgagcctgca gagcttcaac ctggaagttc cagttgacga
    63961 tcacgagcgt atcgagaaag ttcaggaata cgttgctaac tacatcaacg ctgactggat
    64021 cgattctctg actgccactt ctgagcgcag ccgtcgtctg tctccgccag cgttccgtta
    64081 tcagctgact gaacttgcgc gcaaagcggg caaacgtatc gtactgccgg aaggtgacga
    64141 accgcgtacc gttaaagcag ccgctatctg tgctgaacgt ggtatcgcaa cttgcgtact
    64201 gctgggtaat ccggcagaga tcaaccgtgt tgcagcgtct cagggtgtag aactgggtgc
    64261 aggcattgaa atcgttgatc cagaagtggt tcgcgaaagc tatgttggtc gtctggtcga
    64321 gctgcgtaag aacaaaggca tgaccgaaac cgttgcccgc gaacagctgg aagacaacgt
    64381 ggtgctcggt acgctgatgc tggaacagga tgaagttgat ggtctggttt ccggtgctgt
    64441 tcacactacc gcaaacacca tccgtccgcc gctgcagctg atcaaaactg caccgggcag
    64501 ctccctggta tcttccgtgt tcttcatgct gctgccggaa caggtttacg tttacggtga
    64561 ctgtgcgatc aacccggatc cgaccgcaga acagctggca gaaatcgcga ttcagtccgc
    64621 tgattccgct gcagccttcg gtatcgaacc gcgcgttgct atgctctcct actccaccgg
    64681 tacttctggt gcaggtagcg acgtagaaaa agttcgcgaa gcaactcgtc tggcgcagga
    64741 aaaacgtcct gacctgatga tcgacggtcc gctgcagtac gacgctgcgg taatggctga
    64801 cgttgcgaaa tctaaagcgc cgaactctcc ggttgcaggt cgcgctaccg tgttcatctt
    64861 cccggatctg aacaccggta acaccaccta caaagcggta cagcgttctg ccgacctgat
    64921 ctccatcggg ccgatgctgc agggtatgcg caagccggtt aacgacctgt cccgtggcgc
    64981 actggttgac gatatcgtct acaccatcgc gctgactgcg attcagtctg cacagcagca
    65041 gtaatctcgt catcatccgc agctttgcgc tgcggatatc tgaaccggaa atagtcacta
    65101 tttccggttt tttattctct taatttgcat taatcctttc tgattatctt gcttaactgc
    65161 gctgcatcaa tgaattgcgc catttcactt tgcatactta ccactttgtt ttgtgcaagg
    65221 gaatatttgc gctatgtccg caatcactga atccaaacca acaagaagat gggcaatgcc
    65281 cgatacgttg gtgattatct tttttgttgc tattttaacc agccttgcca cctgggtagt
    65341 tccggtgggg atgtttgaca gtcaggaagt gcagtatcag gttgatggtc aaacaaaaac
    65401 acgcaaagtc gtagatccac actcatttcg cattctgact aacgaagcag gcgaacctga
    65461 gtatcaccgc gtacagctgt tcacgacggg cgatgaacgc ccgggcctga tgaacttccc
    65521 gtttgaaggg ttaacctcag gatcgaaata cgggacagcc gttggcatca tcatgtttat
    65581 gctggtgatt ggcggcgcgt ttggcattgt gatgcgtaca ggaaccattg ataacggtat
    65641 cctggcgctt attcgccata cccgcgggaa tgaaattctc tttattcctg cgctgtttat
    65701 tctgttttca cttggcggcg cggtatttgg tatgggagaa gaggccgtcg cctttgccat
    65761 tatcatcgca ccgctaatgg tccggctggg ctatgacagt attaccaccg tcctggtgac
    65821 ctatattgcc acgcaaatcg gttttgccag ttcgtggatg aacccgtttt gtgtggtcgt
    65881 tgctcagggg attgccggcg ttccggtgct ttctggctcc gggttgcgca tcgtggtgtg
    65941 ggttatcgcc actctgattg gcctgatctt taccatggtg tacgcctcac gagtgaaaaa
    66001 gaatcctctt ctgtcacgcg tgcatgagtc cgaccgcttc tttcgtgaaa agcaggcgga
    66061 tgttgaacaa cgtccgttta cctttggtga ctggctggta ttgattgtcc tgaccgccgt
    66121 aatggtctgg gtgatttggg gcgtgatcgt taatgcctgg tttattccag aaattgccag
    66181 ccagttcttc accatgggtc tggtgattgg catcatcggc gttattttcc gccttaacgg
    66241 catgacggtt aataccatgg cttcatcctt taccgaaggg gcgcgaatga tgatcgcccc
    66301 tgccctgctg gtgggtttcg ccaaagggat tttgctgctg gttggtaatg gtgaagcagg
    66361 tgatgccagc gtgttaaata ctatcctcaa cagcattgcc aatgccatta gcggtctgga
    66421 caacgcggtc gcggcctggt ttatgttgct cttccaggcg gtatttaatt tcttcgtgac
    66481 gtccggttct ggtcaggcgg cgttaaccat gccgttactg gcaccgcttg gcgatctggt
    66541 cggtgttaac cgtcaggtta ccgtgctggc gttccagttt ggtgatggct tcagtcacat
    66601 catttacccg acctcagctt cgttaatggc gacgctcggt gtttgccggg tggacttccg
    66661 taactggctg aaggtgggcg cgaccctgct tggactgctg tttattatgt ccagcgtcgt
    66721 agtgatcggc gctcagttga tgggctacca ctaaaaatgt taagagccgc aattgcggct
    66781 cttttttcat tctgccgttt cagtctctac ggcttcattt ttggcattgc gcttcatcca
    66841 caacgctagc gctttcagcg agtctggagt gaactcatcg cagcgtgcgg tgatttcttc
    66901 cggcgtcagc cagcaaactt cactgacttc atcttcctgt agggcgaagg gaccgtgaga
    66961 gacgcagctg aacaatgcgc cccagacacg gcaattttta tcttcgaaat agaactgccc
    67021 gtgctcggca aaggggacac cggcaatgcc caactcttct tccgcttcgc gacgcgcgga
    67081 ttccagcagt tgctcatcgg cctgaaccac accgcctgcg gtcgcatcta acatgccggg
    67141 taaaaagtct tttgtctcgg tacgacgctg gaccagaatt ttgcccatgc catcatgcac
    67201 gacgatgtaa gttgcacgat gacgcagaca ctgtgcccgc atttgctccc ggctggcttg
    67261 tgcaatgact tcgttctctt cattgacaat atccacccat tcagtacttg ccaaacgacg
    67321 ctgttccatt atcaggaaac cttctttttc tggcgctctt acggcgcatt ttgagttgtg
    67381 ggtaaattac ggattaatcg cgacctgcgc aatgatactt tgatcattga gtgcgattac
    67441 actaagtacg tcattatcca gcatgccata actcgccgga ttaccgcctt tcggaatact
    67501 caccgagccg gggttgaagt ggaaaatctc cccccgctgt tctgctactg gcagatgggt
    67561 atgaccgtaa accagcacat cgttctggtt taaagcaggc agattttccg ggccaaaaag
    67621 atgaccatgc gtcaaaaaca gacgttgttt ttccagtaat acctgttgcc acggcgcggt
    67681 tatcgggaaa tgcagcagca tttgatccac ttcgctgtcg cagttgccgc gcacagcgat
    67741 aaccttatgt gccacttcat taagccgttc agcgactttg gctggcgcgt aaccctccgg
    67801 taaagcatta cgcgggccgt gattcaacac gtcgccaagg atcaccagcc actgggcacc
    67861 gctttgggca aacaactcca gaacacgttc cgtcgccggt aacgacccat gaatgtccga
    67921 tgcaaacatc agtttcatca ctcactcctc gtcgaaaaaa cgtaagctat gatactggat
    67981 tctgtcgttg ctatcagcca gattgcttcg ccgaaagtgc aataaaacgc tggacagacg
    68041 ctcgctgcca ctggaatgtt gcataatcca ccaggcgttc tggcacctca tcgccatgta
    68101 gtaccaggcg gttaatcatt agcgccagat cagtatcagc aatgcaccat tcaccaaata
    68161 aattcggctg acccagtact aacaaatgtt ctgccatcgc gaacagtttc tctgcactgg
    68221 cttttccctc ggccgttagt ggcgcttttt tcgcccccgc aaagacaaca tccgtcggac
    68281 gctcttcgcg gatgggcatc agatcgctgc gcaaccaggc ctgaatctgt cgtgcacgcg
    68341 cacgattttc taaatcaagc ggataaatac gttcccaggt cggtggcgca aatcgatctt
    68401 ccagatactc cgcaatggca gaagattcac tcaactcaaa atcatcgatt tgtaataacg
    68461 gcacacggcg tgtctgaccg taaccttgcc acgtcggttg caaatgttca ccgctgtcga
    68521 ggtcgatggt cttgatatga aacgacaggc ctttttcctg caacgccacc caggcggata
    68581 acacataagg ggagaaacag tgggcatctg accaaagcgt gatagcgggt ttactcatta
    68641 tgtcctcggc aataaagggg gctggctatc aaactataga gcctttttgt cactgtcacg
    68701 tgacgaatac tcacgtaagt cgccgcgctt tctatacttg tggtgttcag atgattagac
    68761 aaccggagct gcaatgatcg atctctattt cgccccgaca cccaatggcc acaaaattac
    68821 gctgtttctc gaagaagcag gtctggatta tcgcttgata aaggtagacc tggggaaagg
    68881 cggtcagttt cgcccggaat ttttgcgcat ttcgcctaac aacaaaattc cggcaattgt
    68941 tgatcattct ccagccgatg gcggcgaacc gctaagcctc tttgaatctg gtgccatttt
    69001 gttgtatctg gctgagaaaa cggggctctt tttgagtcat gaaacgcgtg aacgcgccgc
    69061 cacattacag tggttattct ggcaggtagg cggactgggg ccgatgcttg ggcaaaatca
    69121 tcattttaat cacgcagccc cccaaaccat tccttacgct attgaacgtt atcaggttga
    69181 aactcagcgt ctgtaccatg tactgaacaa gcggctggaa aactcgccct ggctgggagg
    69241 cgagaactac agcattgcgg atattgcctg ctggccgtgg gttaatgcct ggactcgcca
    69301 gcgaattgac ctcgcaatgt atccggcagt caagaactgg catgagcgga tccgttcgcg
    69361 ccctgccacc gggcaggcac tgctaaaagc acaactcggt gatgagcgtt cggatagtta
    69421 acagaaacag gttctcgtgt attatttcat cctaagtaaa acaacggaga acctgcaatg
    69481 gcacaacctg ccgctattat tcgtataaag aaccttcgtt tacgtacgtt tatcggaatt
    69541 aaggaagaag aaattaacaa ccgtcaggat attgttatca atgtgacgat ccactacccc
    69601 gccgataaag cgcgcactag cgaagatatc aacgatgcgc tgaattatcg caccgtaacg
    69661 aaaaacatta ttcagcatgt agagaataac cgtttctctt tgctggaaaa attaactcag
    69721 gatgtgctcg atatcgcacg tgaacatcac tgggtgacgt atgctgaagt ggagatcgat
    69781 aaactgcacg cgctgcgcta cgccgattcg gtatccatga ccttaagctg gcagcgttaa
    69841 ccgccatatc gggaggctgc atgaacatag tgatcaccgg agggacggga ttaattggtc
    69901 gccatttgat tccacgtttg ctggagctgg gccatcaaat cacggtaatg acgcgtaacc
    69961 cgcagaaagc cagttccgtt ctcggccctc gggtgacact atggcaaggg cttgccgatc
    70021 aaagcaacct caacggcgtt gatgcggtaa tcaacctggc cggagaaccg attgctgata
    70081 aacgctggac tcacgagcaa aaagagcgtc tctgccaaag ccgctggaat atcacgcaaa
    70141 aactggtcga tttgattaat gccagcgaca cgccaccgtc ggtactcatt tccggctcgg
    70201 caacgggcta ttatggcgat ttaggtgaag tggtggttac cgaagaggaa ccgccgcata
    70261 acgaatttac ccataaactc tgcacccgct gggaagaaat tgcctgccgg gcgcaaagtg
    70321 acaaaacgcg agtgtgcctg ctgcgtaccg gcgtagtgct ggcaccggat ggcggtattc
    70381 tcggtaaaat gctgccgccg tttcgtcttg gcctgggcgg gccgattggt tccggtcggc
    70441 agtatctggc ctggattcat atcgatgata tggtcaacgg cattctctgg ctgctggata
    70501 acgagctgcg cgggccattt aatatggttt cgccttaccc gatacgcaat gaacaatttg
    70561 cccatgcgct cggtcatgcg ctgcatcgcc cggctatttt gcgcgtcccc gcaaccgcca
    70621 ttcggctgtt aatgggcgaa tcttcagtac tggtattagg tggacaacgc gcgctgccta
    70681 aacggctgga agaagcgggt tttgcgtttc gttggtacga tttagaagag gcgctggcgg
    70741 atgtcgttcg ctgatgtggt ttactgcaaa catccgccag ttaactcccg gtgttacagg
    70801 gttagtggct ttgcgcgata agatcgtctg gtgaaagtcg ggtcagcgtc aggatgactt
    70861 cgtggtcaaa tcctttttcc agcatctcct gagcaatacg cagagcttct tcgtgtttgc
    70921 cctgcattgc gccttcttca cgtaatctgt cagcaatggt catcagtttc tccttttctt
    70981 gtggtgcgcg ttctgttatc tcaccaataa atgcacggaa acgctgggca tctcccgttt
    71041 gtaatacata attaaacagg gcttttagct gtctgtcatt agtgttccct gtaactagca
    71101 gcgaaacaat ttggtcgact aatcccaaca gatcgcgctg acgaatatgt ttctgaatta
    71161 actccaacag cgccattttg cgatgttgca taatctcgtc atccggcacc acggtaatat
    71221 ccaccaacgg aaaagccgat gaatatattt tacgggcaat cgcaggctcg gcaaattcat
    71281 caagccagca gagtgaataa ggataaggac ttctgcaacc atgataaaac agcatcggga
    71341 tcaccaatgg aagctcttta tagcccgcat caagatggtt ttgcatcgcc gcaatggaat
    71401 aacgcatcat gcgaaaagcc attaattctt ccggcttact ttggtgctct atcactacat
    71461 aaatataacc cactccctcc tgcgttttca cagaccacaa gaggtcggaa taatattgcc
    71521 gcaggtcttc atcaataaaa ctgtttggtt ccagtttaag cgtcgttaaa tcacacagtt
    71581 tgcgcagcgg cgcggtaaga tgaatatcaa taaaatcccg cgcggtgtct ggatggcgta
    71641 aaaaagattt aaataccgca tcatgcggcg tggaagttgt cgatattgtc atggcgttcc
    71701 gtcacccctc aaatttaaga tgacgcgaca ataaccacga ttaacaccca atgcagcagg
    71761 cagtttttat ctttggacag cgaattcaga agtattttta gcggactgca acttttacat
    71821 ttcagcggaa gctgactcgc aaattcccct tgaaaatact ccggcagcgt tagccaccgg
    71881 agaggcaatt atttcagcga acctttaagg aactgttgca gacgagggct ttgcgggttg
    71941 ccaaataact gctccggcgc gccttcttcc tctattttcc cctgatggag gaaaatgacg
    72001 tgagtagaaa catgacgagc aaagcccatt tcgtgagtca ctaccaccat ggttttcccc
    72061 tcttctgcca gctgctgcat aatgcgcaac acttcgccta ccagttcagg atcgagcgcc
    72121 gaggtaggtt catcaaacag taaaacttcc ggttccatcg ccagcgcccg cgcgatagaa
    72181 acacgctgtt gctgaccgcc ggaaagatgc accggatatt tcccctgtgc acgttcgtct
    72241 atcccgactt ttgccagata cttcaccgcc cgctcgcgcg cttcctgctt gctcaggccc
    72301 aacacctgaa tcggcgcttc catgacgttt tccagcaccg tcatatggct ccagagattg
    72361 aagtgctgga ataccatcgt caggcgtgtg cgcagtaagc gcagttgatt tttatcggcg
    72421 actttgagtt gaccgtcttt gtcgcgcacc agattgatcg tctggccatt gaccacgatc
    72481 gccccttcac tcggtttttc gaggaagtta atgcagcgca gaaaggtact tttccccgat
    72541 cccgacgatc caatgatgct tattacatct ccggcattcg cttgcagtga cacccctttc
    72601 agcacttcgt gttcgccata gcgtttgtgc aaatcgataa cgtttaattt attctcggac
    72661 atcgtattct cagtgcgttg aagaaggttt cacatgctgc aaccagcgtt tttccgctct
    72721 gcgaaagagg ctgatcagga cataagagat gattaaatag agcaccgcgg caatgccgaa
    72781 ggcagtaaaa ggttgatacg tggcggcgtt aatatcgcgg gctattttca gcagatccgg
    72841 caccgtggca gtaaatgcca acgcagtaga gtgcagcatc aggatcactt cgttgctgta
    72901 tgccggtaac gcaatacgca gcgcagaagg caaaataatg cagcgataca ttttaaaagt
    72961 cgagaagcca taggctctgg cggcttcaat ttccccatgc ggaaccgaac ggattgcccc
    73021 agcaaaaatc tcggtagtgt aagcgcaggt gttaagcgtc agcgccagca cggtacagtt
    73081 caggccactg cggaaaaagg cgttaagaaa ctcggtcccc ttaacaatct ccagcgtata
    73141 catgccggaa tagaacacca gcaactgaac atacagcggc gtaccgcgaa aaatataggt
    73201 aaataaccag attggaaact ggatgtattt attactggag acacgaccaa tcgccagaaa
    73261 cagcgccagg actccgccta tcactaccga caaaataagc agccacaggg tgatcgccac
    73321 accagtaaag cgataaccgt cggtccacag cagcggtttc cagtattcat gtaagatttc
    73381 gatcacagat cagccctctt cacacccacg gagtagcggc gctcaaggaa cagcagcaca
    73441 ccattggaaa cggtggtgaa aaccaagtaa atcacgccac agacgatggc gaaatagaac
    73501 ggttcccagg tacttttgcc tgccagctgc gtggctttga ccacatcttc caggccgagt
    73561 aacgaaacca gtgcggtaga tttgaggatc acctgccagt tgttgccaat gcctggcagg
    73621 gcgtaacgca tcatcgccgg aaacatgatc cgccgaaaca cttgcccacg agtaaaaccg
    73681 aacgccgtcg ccgcctctat atgtcctttc ggcactgcca taaaagcacc acggaacgtt
    73741 tcggtaaaat aagcaccgta gataaaaccg agagtgataa taccagcgac catcggatcg
    73801 atatcaatct gcccgacgcc catcgcctcc gtcaccgtgt ttagcgcaat ctgcaaaccg
    73861 tagaaaatca gcagcatcaa cactaagtcc ggcacgccac gaatcagcgt ggtgtaccct
    73921 tcgaaaatca gccccgaaag ccgattttgc gagagcttac cgccagcgcc aattaaaccg
    73981 atgattacag cgagcactac agagctgata gccagctcca gcgtgacgag cgcaccctgt
    74041 aaaataacac ctgaaaaccc atacaacatg ctgcctgtcc tgtcgtgtgt ggtgaattac
    74101 cgcctcttgt ctccctccgg ttacccgaag ggagcgggct ttctttcacc gatggcgatt
    74161 aaccaccata aacatcaaaa tcgaagtact ttttcgctaa tttctcgtaa gtaccgtcag
    74221 ctcgcatttc ggcaaaggct ttgttcagtg cctcgcgcag ttcgttatct tctttacgca
    74281 ggcccatgcc ggtccctacg ccaaacagtt tttcatcttt aacagacggg ccaccgaatt
    74341 tgtaatcttt accgacaggt tgtttgagga aaccttcgct ggcagcgacc tcatcctgga
    74401 acgcggcatc aatacgtccg gcagtcaggt cagaataaat gttgtcctgc ccctgatacg
    74461 agacgatttc aatgcctttt ggtgcccagt gttcgttacc gaacgtctcc tgggtggtgc
    74521 cctgcaatac gcctacccgt ttgcctttca gcgactcgac tgtcggctga atgtcagaat
    74581 ttttcgccac caccagacga gaatcggcag cgtacagttt gtcggtgaag gctatttctt
    74641 gctgacgttt ttccgtaatg gaaagcgatg acatgatggc gtcaatcttc ttcgctttta
    74701 aggacgggat taacgcatcc agcggatttt cgacaaacgt acattgcgta ttgatgcgtt
    74761 tgcataattc ctttgccaga tcgatatcga agccaaccag ttcgccttgt gaattttttg
    74821 attcaaatgg cgcataggtc gggtcggtac cgatgcggat tttttgcgga atcgcagcaa
    74881 atgccgcagt tgcgctggag aaggccagaa ccagagagag cgatagcacc agttttttca
    74941 taatgtcctc aactgacagt ctttttatag ggaatattta caggtttgtt acacttatcg
    75001 tgccataaaa ttgaccatca aggcaaaata tctcgccccg ttagacgtat tatttttcat
    75061 taaaaatgct taagtatgca tttcaatgca ccacgatagt gcaacaaacc tggcgtgcac
    75121 catgatgatc attccatcag gtacagcttc cctgcgacgt atcagtcacc gtagacatta
    75181 aagtcaaaat actttttcgc catcttgtcg taggtgccgt cctgacgcag ctctccaagc
    75241 gccttattga aagcagccgt cagttcagca tcatctttac gtagccctac accggtgcca
    75301 tcaccgaagt attttttgtc tttcactgat gagccagcaa aggcgaagtc tttaccagca
    75361 ggttgcttga ggaatccttc gctggcagca acttcatcct gcagtgcagc atccagacgt
    75421 cctgcagcca gatcggaata gaccaaatcc tggttggcat aggccaccac atccacacct
    75481 ttgctacgcc aggtctcgtt agcgtaagct tcctgggttg atccctgcag cacaccaaca
    75541 tgtttacctt tcagtgaatc cagcgttggc tgaatcggcg aacctttggc ggcaatcaga
    75601 cgagaatctg cggcgtacag cttgtcggag aaggcaatct cctgctgacg tttatcggta
    75661 atggaaagcg acgaaataat ggcgtcgatt tttttcgctt tcagtgaggg gatcagcgcg
    75721 tcaaagtcac tggcaaccca ggtacatttc acctgcatcc gtttgcacat ctcgttaccg
    75781 agatcgatat caaagccaac aaaatcacct ttagcatctt tcgacgagaa cggcgcatag
    75841 gtggtatcgg ttccgatacg taccgtctcc ggtagcgccg catagctgga agccgctgcg
    75901 gagagaccga ctaacaaaga cagagcgaga atcgacttct tcatacataa ccctcaagtg
    75961 aaaatggctt ttttatgttg tgtattgtgt tgtgtgtttg caggctattt catgcaggtc
    76021 ttatgccatc ttgacgacaa cagtaacatt caacgttaaa tgtgttaata agacgttgca
    76081 ttattgtcct gaagttgaag atagcaggta tagcggttga atcgcagcgt ttcgattgaa
    76141 tggcagaaac aaaatgtcga ggatttgatc gcggttgcaa aattgccctg aaacagggca
    76201 acagcggagt tatgcgccct gccagcgggc aaagagatct tcaggaaggg ttatcgcaaa
    76261 ctggtcaaga acacgattaa ccgtctgatt tatcacatca tcaagggatt gcgggcgatg
    76321 ataaaacgcc ggaacgggag gcataatcac cgcaccgatt tctgctgcct gagtcattaa
    76381 acgcagatga cctaagtgca atggtgtttc acgcacgcag agcaccaacg ggcgacgctc
    76441 tttcagcaca acatctgccg cacgggtcag taagccatca gtatagctat ggacaatgcc
    76501 ggacagggtt ttgattgaac agggtaaaat caccatcccc agcgtctgga aagaaccgga
    76561 agagatgctg gcggcaatat cgcgcgcatc atgcgtgaca tcagctaatg cctgcacttc
    76621 gcgcagggaa aaatccgttt cgagggataa ggtctggcgc gccgcctggc tcatcaccag
    76681 atgcgtttcg atatctgtga catcgcgcag aacctgtaat aaacgcacgc cataaatcgc
    76741 gccgctggca ccgctgatgc ctacaatgag tcgtttcata aatagttgcc ctgtcagact
    76801 tgcaggcaga ctttgcagga tttcggcggg agttgcaagt cagggtgcca gaccggcacc
    76861 ctgagcgaag gcatcatcct tcgttatgca tttcgagatt ttccacttcg ttctgacgtt
    76921 gcactgcttt ggcgtcgtca ttacgtaacg tatcgaggaa atcgaggtag ccctgatcaa
    76981 catctttggt gacgtagacg ccgttgaaca ccgaacattc aaactgctga atatccgggt
    77041 tttcagcgcg aacggcttcg atcagatcgt tcagatcctg gaaaatcaac ccgtcagcac
    77101 cgatgatctg gcgaatttca tctacttcgc gaccgtgagc gatcagttcc gtggcgctcg
    77161 gcatatcaat accataaacg ttcgggaagc gaatttccgg tgccgcagaa gcgaggtaca
    77221 ctttcttcgc tccggcttcg cgtgccatct cgataatctg ctcagaagtg gtgccacgga
    77281 cgatggagtc gtcgaccagc aggacgtttt tatcgcggaa ctcggcgcgg ttggcgttca
    77341 gtttacggcg cacggactta cgacgcagct gctggcccgg catgatgaag gtgcggccaa
    77401 catagcggtt tttaacgaag ccctggcggt acggcttgcc cagaatacga gcaatttcca
    77461 gcgcgatatc acacgaggtt tccgggatcg ggatcaccac gtcgatatcc agatcttccc
    77521 attcgcgggc aattttctcg cccagtttcg tgcccatatt cacacgcgcg ctgtaaacgg
    77581 aaattttgtc gatgaacgag tccgggcgag caaagtatac atactcaaac aggcacggat
    77641 tgctgaccgg attgtcagca cattgacggg taaacaactg cccttcttca gtgatgtaaa
    77701 tcgcttcgcc cggcgcgacg tcacgcagga aatcaaatcc cagcgtatcg agcgcaacgc
    77761 tttcggaagc gaccatatat tctgtacggt tctcgtcaat atcacgtttt cccagtacca
    77821 gcggacgaat accgtttgga tcgcggaaag caaccatacc gtggccgata atcatcgcca
    77881 cacaagcata cgcgccgcgg attaagcggt ttgtagcagc gatggcggcg aaaatgttgt
    77941 cggcttccag cgggtagtgg cggaagttgt ccagctcgct ggcgaagata ttaagcagaa
    78001 tttccgagtc ggaagtggtg ttgatgtggc ggcgtttttc ttcaaacagt tttttacgca
    78061 actcgtgagc gttggtcaga ttgccgttgt gggcaagcgt aatgccatac ggggagttaa
    78121 cgtaaaacgg ctgcgcttca gaggcgctgg agctgccagc cgtggggtaa cgcacatgac
    78181 caatgcccat attgccctgc aaacgctgca tatggcgagc ttcaaataca tcgctcacca
    78241 gcccgttcgc tttacgcaaa cggaagcagt tattggcatc tatggtgatg atgccggcgg
    78301 catcctgacc gcgatgctga agcaccgtta aggcatcata aatcgactgg ttaaccggca
    78361 taacaccggc gataccgaca ataccgcaca tacgtctttt tcctcgttaa gccacatctc
    78421 agagcactta cgctctgggc aagaaacttg acgagctttg cagataatca aaaaaccatc
    78481 tgatgataaa actgaactgc gggatcagct gtgatttact ccagtcttcg cttttcgaca
    78541 ccccggtaaa ggagtcgaga aagaagagaa tggcagcaac aatcaacaca ccgcgcaacg
    78601 caccgaaaca gacgcccagc acccgatcgg tgcctgacaa ccccgttttc tccaccagct
    78661 ggcctatcac gaagttcacg atagcaccaa cgatcagggt agcgataaac agtaccgcga
    78721 tggcaatccc atttcgaacc agttcgtctt caaagcccgt aaaccagact gacaggtaag
    78781 tgtagtaatg actggcaaca aagaaagcac aaccccatgt caccagcgat aacgcttcac
    78841 gaacaaagcc gcggatcagg ctaaccagag aggaaaaagc aatcaccgcg attatggcgt
    78901 aatcaatcca gaccatatgt gtcccacgat tttacgccct gtcatcctgt tcggggcgca
    78961 ttctaacaga aaaagaaaac gtttgcgtag ggatttcctt cccgcgcatc aataaaaatg
    79021 gcgctgaaaa aatattcaac gccatcgact ttttatgcct ttgcggcatc ggacaatgcg
    79081 tgtcggatgc ggcgtaaacg ccttatccga cctacggttc taaccctgcg taggcctgat
    79141 aagacgcgcc agcgtcgcat caggcaacgg ctgtcggatg cggcgtgaat gccttatccg
    79201 acctacggtt ctacccctgc gtaggcctga taagacgcac cagcgtcgca tcaggcaacg
    79261 gctgtcggat gcggcgtgaa cgccttatcc gacctacggt tctacccctg cgtaggcctg
    79321 ataagacgcg ccagcgtcgc atcaggcaag accgtattaa ttcggcgtat agcccattac
    79381 cacgccactt aagccagaaa gttgcttcaa ctcacccagc gatcctttca gcttatcttt
    79441 cgaggcatct ggcccaacca gaatacgggt aattttaccc tgcactggcg tggatggcga
    79501 cgtataaacc cgataaccgg caccgcgcag cttaccgaca atctcattca ctttatcggc
    79561 atttttcagc gcccccagtt gcacaacata ggctttaccc gtcggtgcag ctttttcttc
    79621 cacgaccggc ttcggttctg gcttcggcgc aggtggtgct tcaacctttg gtttaggcgg
    79681 ctccaccggt ttcggcttcg gtggggcgac cggtgcaggt tccggttcaa actcggtgtt
    79741 attggctgca atagtggccg gatcgagcga cggtgctgcg gcatcacctg cccgcacctc
    79801 ttccgctgcg ccttccggcg gttgcgtcgg caacgcctgg gtggcggctg gcatcatatc
    79861 aggctcatcg cgatcgcccg cttttggcac cagcgggata gccgcaaact catcctgata
    79921 atgttttttc tgcccgtcca gcagccctgg aagtacaatc acccccagcg ccaccagcac
    79981 gatcgtgccc actaaccgat tctgaaactt acttgccacc gcttctcctc gcgtcaatca
    80041 cttccatgac atgtgcgacc gtgtggaacg aaccacacac cagcacggtg tcttccgctt
    80101 tagcgtccgc cattgcggca tcccatgcct gcgcaacgct atcaaatgat ttgccgttac
    80161 ccaaatgctc aagcagttgt tctgccgtgg caccgcgcgg cccttccagt ggcgcacaat
    80221 accagtcatc aaccacgctt ttcaaccagg ccagagttcc ggcaatatct ttatcatgta
    80281 gcataccgat aaccgccagc acgcgcccgt ttttcggtag cgttttcata cgcccggtga
    80341 gatattccgc cgcatgtgga ttatgcgcga catcaaaaat aacgcgtggc gactcgctca
    80401 caatctggaa acgtcccggc aaaattgcgc tggcaatccc gtcgcgaatg gcattttcac
    80461 tgacctccag cccgctggca cgcagtgccg ccagcgccgt tgcggcattc ggttgcggga
    80521 caagcggcaa cggcaggttt tccagcgtgc cgtgagcatc gctaaacgcc cagtcatgat
    80581 cggtgacgga atagttccac tcaacgcccc gacgttgtaa cagtgcacct ttttcctgcg
    80641 ctacatcagc aatggtagaa ggcatttccg gctcaccgac aattgccggt ttttcgctgc
    80701 gaaagatgcc tgctttctcg cgaccaatac tttcgcgatc tggccccagc cagtcggtat
    80761 gatccagcgc aatgctggtt actaccgcga catcggcgtc gacaatattg gttgcgtcca
    80821 gacgaccgcc cagccctact tccagaatca ccacgtcaag ttgtgcctgt ttgaacagcc
    80881 acaacgccga cagcgtaccg tactcgaaat aggtcaggga aatatcaccg cgtgccgatt
    80941 caatctccgc aaaagaggca gtgtgggccg attccggcaa ttcctgaccc tgcacacgta
    81001 cgcgctcggt ataacgcacc agatgcggcg aactgtagac gcccactttg taccctgccg
    81061 ccatcagaat cgactccagc gtacggcagg tggtgccttt gccattcgta cccgcaacgg
    81121 taaacacaaa tggcgctggt ttcagaacgc caagacgcgc cgcgacctgg ctcacgcgct
    81181 caaggccgag atcgatagtt ttactgtgca ggttttccag ataagaaagc cacgaagcca
    81241 gaggcgacgc ggcttgagga gtgcgtttga taatcatggt atccgctgat tcgttacggt
    81301 gagaatagca aaagggcaga gccagtggcc ctgcccttat cagttatcag gcctcaggtt
    81361 cctgatccgg taccgggggt accactacgc cttcacgcgg cgcttcagga ttcggcgctg
    81421 gcagattcat caacttcgcc agaatgctcg ccagtttcag gcgcatttcc ggacgacgga
    81481 cgatcatgtc gatcgcgcct ttctcgatca ggaattcact gcgctggaat cctggcggca
    81541 gtttttcgcg aacggtctgt tcgataacac gcggaccggc aaagccgatt aacgctttag
    81601 gttcagcgat gttgagatcg cccagcatgg cgaaacttgc agaaacaccg cccatcgtcg
    81661 ggtcggtcag cacggagatg tacggcaagc cgcgctcctg catttttgcc agtgccgcag
    81721 aggttttcgc catctgcatc agcgacatca gtgcttcctg catacgtgcg ccaccagagg
    81781 cggagaagca gatcagtggg cagttatctt ccagcgcctg ctcaacggca cgcacgaaac
    81841 gtgcacccac aacagacccc attgaaccgc ccataaaggc gaactcgaat gccgcagcga
    81901 caaccggcat tccatacaga gtgcctttca tcaccaccag cgcatctttt tcgccggttt
    81961 ctttctgcgc agatgccaga cggtctttat acttcttgga gtcacgaaac ttcagcacat
    82021 ctttcggctc aagctcgcta cccagctcca caaggcttcc ttcatctaac aggctatgca
    82081 ggcgattacg cgctgtcata cgcatgtgat ggtcacactt cggacagacc tcaagattac
    82141 gttccagctc agcgcggtat aaaacctgac cgcagctatc acacttagtc cacacccctt
    82201 caggaatgct cgccttgcgg gtgggagtaa tgttgctttt aattcgttca atccagctca
    82261 ttagggacct ttctgtctga acctggttcg atgccagttt tatctttggg gacgcataat
    82321 gccatttttg cccccaacag accatgaatg ttgcacatta aaacataaca gcccgaaact
    82381 ttggataaaa aagtggtcga accgcggagt tactttttat tttgcggcgc gtgccgcagc
    82441 gcgtttgtga cggattattt cgatgacgcc cggcaaaata gaaaccacaa taatcccgac
    82501 gatcagcagc ttaaggttat cctgaaccat cgggattgta ccgaagaaat agcctgcgta
    82561 ggtaaaaagc agtacccaca acagtgcgcc gatcacgtta taagcggcga aatgacggta
    82621 cgacatgtgg cccattcccg caacaaacgg ggcgaacgtt ctgacgatcg gcacaaaacg
    82681 ggcgagaata atcgttttgc cgccatgttt ctcataaaac tgatgggttt tgtcgagata
    82741 actgcgacgg aaaattttcg aattgggatt actgaataac ttttcaccga acaaccgccc
    82801 gatagtgtaa ttgaccgcgt cacccacaat cgcggcgatc agcatcagca ccaccatcat
    82861 atggacgttg agatcgttgg tttccagtga tgccaacgct ccggcaacaa acagcaatga
    82921 atcacccggt aaaaacggcg ttaccaccag accggtttca cagaacaaaa ttaagaacaa
    82981 aatggcataa acccagacgc cgtactccgc gaccagctct gccagatgca cgtcaatgtg
    83041 caggataaaa tcgatgagga aataaatcag gtccatattt gcctatgcct tgtactcgtc
    83101 atctttcagg ctgtaactac gttggctaca gcctgaaatg ttccgagcat tattctcgtt
    83161 agtccgccag aaatagcggg cccattggcg gttttggaag atcaaaccgg tcagggtaat
    83221 ccaccgcgac cagatacagc ccttccgctt ttgccgttgc tgccgccagc gttctgtcct
    83281 ttgccgccag caactctgct atccagctct ccggctggtt gtgggcaccg acttccatca
    83341 ggctgccgac aatattcctg accatatgat gtacaaaggc attcgctttg atatcaacca
    83401 ccacataagg accgtgacgc gtgacattaa tatgcataac gttgcgccac ggggttcggg
    83461 actggcactg caccgcacgg aacgaggtga aatcattctc gcccagcaag cattgcgcag
    83521 cccgatgcat ccgttcagcg tccagcggtt cgtaaaaatg ggttacccct ttactcagta
    83581 ccgccgggcg cagccgatga ttgtagatga tatagcgata acggcgagcc gtggcgctaa
    83641 atcgggcatg aaaatcatca ggtacagctt taacccaacg cacggcgatg tcaccaggta
    83701 aattcgcatt tacgcccaac gtccacgcag cgtctttgcg ctgcgcggtt gtttcgaaat
    83761 gcaccacctg cccggtaccg tgtacccctg cgtcagtacg cccggcgcag aagacggtga
    83821 tgggttcgtt cgccacctgg gagagcgcct tttccagctt ctcctgcaca ctgcgaactt
    83881 cgttctgccg ttgccagccg taatacttac tgccgtcgta ctcaatgccc agcgcaattt
    83941 tataaactgg cggttgttgc tggtcggaca ttagtacaga tactcctgca ccagtttctc
    84001 ggcgattttt actgccatca gcgcgccgcc aaagcgaacg ttatcggcca ccgaccagaa
    84061 ctggacttgc tccggcatac cgtagtcatt acgcacgcag ccaacagaaa gatgcggcgt
    84121 acccgaagca tcccctacct gagttgggaa ttcgttctct tcagagagca caatatcttc
    84181 gccttgaaca aacgcatcac gcgcttcttc tgctgccagt ggacgcagag cttcaaagtt
    84241 gaccatctgg gcatgaccgt agaataccgg tgcctggacg acgcttgccg aaatcatcag
    84301 tccttcgtcc tgcaggattt tgcgtacttc gtcaacgata cgacgttctt cacgcacgct
    84361 accttcgcta tccggcagta acggcagcat gttgaacgcc agctgacgcc cgaagaaatc
    84421 ttcttcgtca atcggaatgc cgttgagcaa tttagcactc tgccccgcta acgcatcgac
    84481 cgcttttttg ccctgggcgg aggctgaaat caggctggta acgctgatac gtgataaacc
    84541 gccctgatcg attaacggtt tcagtgccgc cagcagctgg ctggtcagac tgtccggtac
    84601 ggcgatgaca ttccggttgc ggtaatcggt cagtacaaac gggtttactt ccggcaccac
    84661 cagcggtacg tcgggttcga gagcaaacaa tccgctgctg tcgatcacca ggcaacctga
    84721 gttggtcgct tcttcaaccc aggctgcggt agcttctttg cctgcgacaa aaaatgccag
    84781 ctgcgcctgc gtccagtcga attcagcagc atcctgcacg gtgattgtct taccaccaaa
    84841 gcgcagttgt tcgcctgcgc tttcgttacg tgccagtgca taaatttccc caaccgggaa
    84901 ctgacgttca gccagcgttt caagcagggc ttcgcccaca gcgccagttg cgcccaggac
    84961 ggcaatgttc cagccttcag acatggtggt ttactccaga aatagcaaag ctccctgcca
    85021 atgttacagc agagagcatg aagaagagat taacgtgccg gatgatgaac ggcgttaaaa
    85081 cccagtttac acagcaatga tgccgcactg gcgtcatcac aaattacata cagagatgac
    85141 cattcacggc gctcaagata gtttttgcgc agtttatcga actcacccgg tatcccggcg
    85201 actttacgca gcggtgcgtc atcgcggcgc acatcataca ccaaatgcac cagccttttc
    85261 agcgttggtt gatcgagcgg gccatgcagc gtaatgcgac caaactctgg cgcgggcagt
    85321 aatgtatcca gcgcaatgtg ctgttcatgc ccaataaact tgctataggc ttcaaacact
    85381 tgcgtagtac cgcgtgcttt accttccagg gtatagcctg caatatgcgg cgtgccgata
    85441 tccacttttg tcagcaactc cacgttaagt tccggttcgc cttcccagac atccagcact
    85501 acgcttaact tctggccttc attcaggcag gtcagcagcg cggtattatc gacgactgcg
    85561 ccacggcagg cgttaatcag aatcgctccg ggcttcaggt tacggatcag tttttcatcc
    85621 gccagatgca gcgttttgta cggcccgtct ttgaagagtg gcgtatggaa agtcagaata
    85681 tccgcatgct gaactaattc atccagcgag cggaaatcac cctcatcccc acggtcagcg
    85741 cgaggcggat cgcaaagcaa ggttttaatc cctaacgctt ccagtcgcgc ctgcaatcga
    85801 cgtccaacgt taccaacgcc cacgatcccc accgtacggt cgtgcagtga aaatccatcg
    85861 cgttcggcaa gcatcagtag tgaggagaga acatattcca ccaccgcaat cgcattacag
    85921 ccaggtgcag cggaaaaacc aattcccgcc ttctttaacc atgcttcatc gacatggtct
    85981 gtccccgcag tggcagtgcc aacaaattta atgggttttc ctgccagcaa agattcattc
    86041 actttcgtga ccgaacgcac catcagcgca tccgcgtctg ccagttgagc gacggggatt
    86101 ggacgcccgg gaaccgcgat cacctcaccc aaacggctaa ataagtcgcg ggcataaggc
    86161 atattttcat caacaaggat tttcacgttt gtgttacctg tatgagacga gagttaaccg
    86221 gacaagtgtg ccataatctc gcggccaggc atacttgcga agatttcagg tataaggata
    86281 cgtaatgata caacctattt ccggccctcc tcctgggcaa ccaccaggtc agggagataa
    86341 cctgccgtct ggcgcgggca atcagccttt atccagtcag caacgtactt cgctggaaag
    86401 cttaatgacg aaagtgacct cactgacgca acagcaaaga gcagaactgt gggcgggtat
    86461 caggcacgat attggtctgt cgggagattc accgctgctt tcgcgtcact tccctgccgc
    86521 tgagcataat ctggcgcaac gtctgctggc cgcgcaaaaa agccattctg cccgccagct
    86581 tttagcgcaa ttaggggagt atttacgtct ggggaataat cgtcaggcgg tcacggatta
    86641 tatccgtcat aactttggtc agacgccgct gaatcagctc tcaccggagc aattaaaaac
    86701 cattctcacc ctgttgcagg aaggcaagat ggttattccg caaccgcagc agcgcgaggc
    86761 gaccgatcgt cctctattac cggcggagca caatgcgctc aaacagctgg tgaccaaact
    86821 tgcggcggca acgggggagc ccagcaaaca gatctggcaa tcgatgctgg aactttccgg
    86881 ggtgaaagat ggcgagttaa ttccagcgaa actgtttaac catctggtga cctggctgca
    86941 ggcgcgtcag acgctaagcc agcaaaatac gccgacgctg gaatcactac aaatgacgct
    87001 aaaacaacct ttagatgcca gtgaactggc ggcgttatcg gcatatatcc agcaaaaata
    87061 tggcctttct gcgcaatcat cgctttcttc tgcccaggcc gaggatattc ttaatcagct
    87121 ttatcaacgg cgggttaaag ggattgatcc gcgtgatatt caaccgctgc ttaatccttt
    87181 tccaccgatg atggacacgt tgcaaaatat ggcaacgcgt cccgcgctgt ggatactgtt
    87241 agtcgcgatt atcctgatgc tggtctggct ggttcgttaa ccccgacgaa acgacagtat
    87301 cgtgacaata attcccagca ccgcagagat cgccccggca agaaataccg aagagtaacc
    87361 aaacgtggtc gccagcattc ccgccagcgg cccggagacg ccgagggcga tatcctgaaa
    87421 cgcggcgtaa ccgcccagtg cggtgccgcg aacttgtgag gggacacgtt taaccacctc
    87481 cacgcccagc gcaggaaaga taagcgaaca tccggctccg gttaacgccg cgcccgctaa
    87541 tgcgacccac gcacccgggg cttgccagag cagcaacaag cccaccgttt ctacaagcag
    87601 agagacgatc gccactttca cgccgccaaa acggtccggc atccagccaa acatgacgcg
    87661 catcacgaca aatgcgccgc taaacgcggt aagagtaaag cccgccatcg cccatccttt
    87721 gctggcaaag tagagcgaaa cgaaagtccc gataaccgca aaaccaacgc cttgtagtgc
    87781 cagccctaac cctggtttcc agataagccc gacaacgctc cacagcgatg gacgttctcc
    87841 cgccagggcc ggtactttgc gcactgtgcc gttacaggcc cacgccagta agggtaatgc
    87901 cattgtggtg agcgccagtg cggcaaaacc gtaatggcta tgaatcaaca ggccaagcgg
    87961 tgcaccaaca gcgagggcac cgtaaatcgc cattccgttc catgacatta ctttgccaga
    88021 gtgttttggc cctacgatgc ctaatcccca ggtcagagcg cctgtcagta actggctttc
    88081 accaaagcca agaatcaaac gcccgacgac caacagggca aatttgaacg gtgcggagac
    88141 gggcaaaatc gccgccagca gcaacgcgcc ccccgccaga ccacaagcta acatcccctg
    88201 aagcgccgaa cgttttgcgc catattgatc ggccagtcgc ccggcgtaac cacgcgtcag
    88261 caccgtagcc agaaactgaa tgccgacggc aatgccgacc atggtattgc catagcccaa
    88321 ttcatgatga acaaacaacg ggataaccgg caacggcaac cctacggtca tgtaggtgag
    88381 aaaaaccgca aaagcgatgc ggaagagcga aaaattggca gaagatcgtg tttcggtttg
    88441 gcttacagca gtcatgcatt actccagaat gcagcgcaag gcgaggagta tccccgtctc
    88501 atctctctgg tttcagggtt acggtgcgtt ggcaggaatt aacgcgtacg tcttttcaga
    88561 aggaaatcga caaagcggga agtttgcctg gaacaggcgg cgattgtcaa tgatgtgaaa
    88621 aagggaacca tcgggttccc tttggcgtta gtgccggatg ccgcattggc gcgtaacgtc
    88681 ggatgcgacg ctggcgcgta acgtcggatg cgacgctggc gcgtcttatc cgacctactc
    88741 cgactactcc ggcctactgc gaattaatct ttcagcttgc gcattaccag cgtggcgttg
    88801 gtgccgccga agccgaagct gttagacata acagtggtca gttcgcgatc ggtcgtttcg
    88861 gtcacgatgt tcagacccgc agcctgctcg tccagctctt caatgttgat gctcggggcg
    88921 ataaagccgt gttccagcat cagcagagag tagatagctt cctgtacgcc agcagcgccc
    88981 agagagtgac cggtcatggc tttggttgca gaaatcgccg ggctcttatc gccgaacact
    89041 tcacggatag ctgccagctc cttcacgtcg ccaaccggag tcgaagtgcc gtgggagttc
    89101 aggtaatcga ttggggtatc aacgccatgc atcgccatct gcatacaacg tactgcgcct
    89161 tcgccagacg gagcaaccat gtctgcgcca tcagacgttg cgccgtagcc aacgatttca
    89221 gcatagatgt gagcaccacg cgccagcgcg tgttccagct cttcaaccac caccataccg
    89281 ccgccgccag cgataacgaa accgtcacgg tgagcatcgt aagtacggga ggctttttcc
    89341 ggggtgtcgt tgtatttagt agacagcgca cccattgcgt cgaattcgca agccatttcc
    89401 cagcacagct cttcgccgcc gccagcaaac acgatgtcct gtttgcccag ttggatctgc
    89461 tctactgcgt taccgataca gtgtgcggaa gtcgcacacg cggagctgat ggagtagtta
    89521 acgccatgaa ttttaaacgg ggtggcgagg caggcagaaa cgccggatgc catcgctttg
    89581 gtgaccacat acgggccaac cgctttcagg ccgcgcgggc cgcgcattgc gtcagcgccg
    89641 aacacctgga aacgcgggga gccgccgccg gaacctgcaa tcaggccaac gcgcgggtta
    89701 ttctggtaag cttccggaga gaggcccgca tcagcgattg cctgctccat agaaaggaat
    89761 gcataaatgg atgcgtcgct cataaagcgc acaactttgc ggtcaatgag gccagtggta
    89821 tccagtttta cgttgcccca gacgtggcta cgcatgccgg aatccttcag ctcctgagag
    89881 aaagtgatcc ctgaacgtcc ttcacgcaga gatgccagga cttcctgctg gttattaccg
    89941 atgctggaaa caatgcccag gccagtaatc actgcacgtt tcattcaata cctctgtaag
    90001 tcgcacatag agtaagtttc gaatgcacaa tagcgtacac ttgtacgccg aacaagtccg
    90061 atcagccaat taatagagaa atttgcgcag ccttacacac atcgctaaga tcgagccacc
    90121 gcctgtaaga cgagtaactt acgtgaaaca ctactccata caacctgcca acctcgaatt
    90181 taatgctgag ggtacacctg tttcccgaga ttttgacgat gtctattttt ccaacgataa
    90241 cgggctggaa gagacgcgtt atgtttttct cggaggtaac caattagagg cacgctttcc
    90301 tgagcatcta catcctctgt ttgtggtagc agagagcggc ttcggcaccg gattaaactt
    90361 cctgacgcta tggcaggcat ttgatcagtt tcgcgaagcg catccgcaag cgcaattaca
    90421 acgcttacat ttcattagtt ttgagaaatt tcccctcacc cgtgcggatt tagccttagc
    90481 gcatcaacac tggccggaac tggctccgtg ggcagaacaa cttcaggcgc agtggccaat
    90541 gcccttgccc ggttgccatc gtttattact tgatgaaggc cgcgtgacgc tggatttatg
    90601 gtttggcgat attaatgaac tgatcagcca actggacgat tctctaaatc aaaaagtaga
    90661 tgcctggttt ctggacggct ttgcgccagc gaaaaacccg gatatgtgga cgcaaaatct
    90721 gtttaacgcc atggcaaggt tggcgcgtcc aggcggcacg ctggcgacat ttacgtctgc
    90781 cggttttgtc cgccgcggtt tgcaggaagc cggattcacg atgcaaaaac gtaagggctt
    90841 tggacgcaaa cgggaaatgc tttgtggggt gatggaacag acattaccac tcccctgctc
    90901 cacgccgtgg tttaatcgta cgggcagcag caaacgggaa gtagcgatta tcggcggtgg
    90961 tattgccagc gcattgttgt cgctggcgct attgcgtcgc ggctggcaag taacgcttta
    91021 ttgcgcggat gaagtccccg cgctgggtgc ttccggcaat cgccaggggg cgttgtatcc
    91081 gttattaagc aaacacgatg aggcgctaaa ccgctttttc tctaatgggt ttacttttgc
    91141 tcgtcggttt tacgaccaat tacccgttaa atttgatcat gactggtgcg gcgtcacgca
    91201 gttaggctgg gatgagaaaa gccagcataa aatcgcacag atgctgtcaa tggatttacc
    91261 cgaagaactg gctgtagccg ttgaggcaaa tgcggttgaa caaattacgg gcgttgcgac
    91321 aaattgcagc ggcattactt atccgcaagg tggttggctg tgcccagcag aactgacccg
    91381 taatgtgctg aaactggcgc aacagcaggg tttgcagatt tattatcaat atcagttaca
    91441 ggatttatcc cgtaaggatg actgttggtt gttgaatttt gcaggagatc agcaagcaac
    91501 acacagcgta gtggtactgg cgaacgggca tcaaatcagc cgtttcagcc aaacatcatc
    91561 tctgccagta tattcggttg ccggtcaggt cagccatatt ccgacgacac cggaactggc
    91621 aaaactgaag caggtgctgt gctatgacgg ttatctcacg ccacaaaatc cggcgaatca
    91681 gcatcattgt attggtgcca gttatcatcg cggcagcgaa gatacggcgt acagtgagga
    91741 cgatcagcag cagaatcgcc agcggttgat tgattgtttc ccacaggcac agtgggcaaa
    91801 agaggttgat atcagtgaga aagaggcgcg ctgcggtgtg cgttgtgcca cccgcgatca
    91861 tctgccaatg gtaggtaatg ttcccgatta tgaggcaaca ctcgtggaat atgcgtcgct
    91921 ggtggagcag aaagataagg cggtaagcgc gccggtttat gacgatctct ttatgcttga
    91981 tgctttgggt tcgcgtggtt tgtgttctgc cccactgtgc gcagagattc tggcggcaca
    92041 gatgagtgaa gaaccgattc cgatggatgc cagtacgctg gcggcgttaa atccgaatcg
    92101 gttatgggtg cggaaattgt tgaagggtaa agcagttaag gctgggtaac cagctttggc
    92161 attgtttgtc ggatgcggcg tgaacgcctt atccgaccta agtgtgacgt gtaggcctga
    92221 taagacgcga taagcgtcgc atcaggcatg gtgctccaga cgccgcaact tactgctgtg
    92281 acgcctgctg aaacaggttt tcccacatat cggtcaccag cgcctggtca cgcggcgaca
    92341 attcaccagc accaatggct ttttccagac tctggctaac ggtcgtatgc accgcctgag
    92401 cggagtggtc atcaccactt tccagttctg cgatggctaa tgtcaggtgg ccacgcaaat
    92461 acccactggc aaacaactca tcatcactgg catggtcaac cataccgtcg attaatgcca
    92521 gaatgcgtga ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg tggcgctggc
    92581 ttcagttgag cgcttccggc cacgggaact gttccgccgt aagttccggc gtgtgataat
    92641 aattttgtag tgctttaatg aaacgtgccg ggcgttccgg aatgccattt gttagataat
    92701 ccatcacctg cgcatgtact cgccgttgaa acaccacgcg gtccggttcg aagtcgcctt
    92761 ccagattgtc gcaactgacg ttaaacggat atccagccgc cacgcagaac aaccagtcga
    92821 gcgcctgcgg cttcacttca acatcttcaa actggctttg cgtttgggca tcgcgtccgt
    92881 ccgggcaata ccagtagccg aaatcaacca gctcacggcg cgcttttccg gcaatacacc
    92941 agtgcgaaat ctcatgaatt gcgctggcat aaaagccatg agcaaagacg attcggttat
    93001 acggtacttc cgcatcagca ggaagataga tcggttcgtc gtcgccttta atcagacggg
    93061 tattaaaatc atcggcaaag cagctattaa aaatttcaat caactgctca tagtggtgtg
    93121 tactgttcat tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc tatcgtaaag
    93181 taatttggca ctcatcaccg cggagacgat aacaatcatc gggcggatca gcttttgtcc
    93241 tttgctcaac accagtcgtg aacccatgcg cgcgccgagg aactgcccca ccagcatcac
    93301 aaagcccgtc gcccaaatca ctttgccgcc gagaataaac agcagcaaac caccgatgtt
    93361 tgacgttgcg ttgagtaatt tggcgtgagc cgtggctttg gcgaggttga atccgcacag
    93421 cgtaacaaag gccagcgcgt aaaacgatcc ggctgccgga ccaaagaatc cgtcataaaa
    93481 accgacgcag ccaccggcaa ttaacgcgaa gggtaaaccg tacatccggc gctggcgatc
    93541 ttcttcaccc agctttggca tcagcaagaa atagaggccg atacaaatca ccagaatggg
    93601 caaaatctgc cgtaagacat cagcctgaac gtactgtacc agcagcgcgc cgctcattga
    93661 gccgacaaag gtcatggcga tattgagttt ctgatcgctt aaactaacca ctttgcggcg
    93721 aataaagtag atagtggcgg aaatagagcc gccgcaggct tgcagtttat tggttgccag
    93781 cgcattagcg ggagacatcc ccgccgccat caatgccgga atggtgagta acccaccgcc
    93841 accggcaatc gagtcgataa atcccgccag catggcgaca aaaaagagaa ctcccagcaa
    93901 cagcggggaa accataaaca ggctattaaa cgtttccatt agatcacgtg ctcatccagt
    93961 agcgcctggc aggaaggcgg caacggaggc ggtgtcttct tctcaggctt tgttgttccc
    94021 ggttttggag gttcaaacca gctttgcagt tctgccccgc aaccatcgcc tggtggcggt
    94081 aaaggttgat cttcacactc cagactatcg gcaggacaac gtaatcgtac atgcatatgc
    94141 gcacgatgct ggaaccaggg tcgcactttg cgcaaccagt cgcgatcggt gcccgcatca
    94201 aggcaaagtt gttgtttaat cgccggatta acaaaaatgc gcgtgacgtc tttgtcctgg
    94261 gcggcgagtt tgatcaagct gaaaatttcc ggcttccaca gcgtggggac aacgcgttta
    94321 ccgtcgcggg atactaagtc cagtgcttgc gggcgcaaga gctgcgcgga ggtccagcga
    94381 gttttcggca gttgcagaaa gatatcgaca tccagtccgg tctggtggct ggcatgaccg
    94441 ccgttgaaac gcccaccagc gggcatcccc atatcgccaa tcagcaccgt acccatgccc
    94501 agattgctca cctggctact cagacgctgg ataaacatca ccaggtccgg gtgaccgaaa
    94561 tagcgacgct gatcggtacg catgacctga taatgttcgg actgtatcgg cagcgtgtca
    94621 gcgccgacga tacagccatt agaaaaactg cctatcgatt gtgcgctacc cggcacaggt
    94681 tgggttattt tttgccacgg cgtcgctgcc aggctgacgc tactggcaag cagggccagt
    94741 agcgcaatcg cggttttgtt cattttttac cagcgtggaa tatcagtctt cacatcggca
    94801 ttttgcgccc gctgccgtaa caggtgatcc attaatacga tcgccagcat cgcttctgcg
    94861 atcggcacgg cgcggatccc gacacaggga tcgtgacggc ctttggtgat catctcaact
    94921 tcttcgccaa agcggttaat ggtgcgcccc ggcacggtaa tgctggaggt cggtttcagc
    94981 gccatatggg caatgatttg ctgcccgctg ctgataccgc caagaatgcc gcccgcatgg
    95041 ttgctctgga aaccgtcttt ggtgatttcg tcgcgattct gactgccgcg cagcgccact
    95101 acgtcaaaac cgtcgccaat ttccacgcct ttcaccgcgt tgatgctcat cagcgcatgg
    95161 gcgatgtcgg catccaggcg gtcaaagacc ggctcaccaa gcccggcagg aacgccactg
    95221 gcaacaacgg tgactttcgc gccaatagag tcgccctctt ttttcagcgc gcgcatcagt
    95281 tcatctaacg cgtcgatttt gtccgggtcc gggcagaaga acggattttg ttcaacctgc
    95341 gaccagtctt tgatttccag cggaatgtcg cccatctggg tcaggcagcc gcggatttca
    95401 ataccaaatt tctcggcgag atattttttg gcaatcgccc ctgccgccac gcgcatggcg
    95461 gtttcgcggg cggaagaacg tccgccgccg cgataatcac gcagaccgta tttttgttcg
    95521 taggtgtaat cagcatggcc tgggcggaaa acgtctttaa ttgcgctgta atcctgagaa
    95581 cgctgatcgg tattttcgat caacaaacca atgctggtgc cggtagtaac accttcaaaa
    95641 acaccggaga gaattttgac ctgatccggc tcacgacgct gggtggtata gcgcgatgtc
    95701 ccagggcgac gacgatcaag gtcatgttgc aggtccgctt ccgtcagcgg aatgcctggc
    95761 ggaacaccat cgacgatgca gccgagcgcc agcccgtgcg attcgccgaa ggtggttacg
    95821 cgaaagagtt gtccaattgt gtttccagcc atcacggctc cgttattgtt gtgtttgcgt
    95881 gtttacttaa tctttataaa tcgcgaaatg ttcacgggcc gccagcagct gctctttggt
    95941 gagcataaac acaccatcgc cgccgttatc aaactccagc caggtgaacg gaacatccgg
    96001 atattgttcc ataagatgta ccatgctgtt gccgacttca caaatcagca caccatcatc
    96061 agcaaggtaa tctgccgcgt taccgagaat gcgacgcgtc agtttcaggc cgtcagtgcc
    96121 agatgccagg cccagttctg gctcgtgacg gtattcgttt ggcaggtcgg acatatcttc
    96181 cgcatcgaca tacggcgggt tagtgacaat caggtcgtac tgcactttcg gcaaatcacg
    96241 gaacagatcg gaacgaatcg gaatgacgtt gtggatcaga ccgtgttctt cgatgttctg
    96301 ttcggcaacc gccagcgcgt cgggagagat atctaccgca tcgacttccg cttccgggaa
    96361 ggcataagca caggcaatgg caatgcagcc gctaccggta cacatatcta aaatatgctg
    96421 cggttgcttg ctgataagtc cggcaaattt attgttgatc aattcaccaa tcggcgagcg
    96481 cggcaccagc acgcgttcat cgacgtaaaa ttcatgaccg cagaaccagg ctttattggt
    96541 caggtaagcc accggaatgc gttcgttgac gcggcggatc acgcgctcaa caatacggtg
    96601 tttttcgctg gaggtcagac gcgcggtgcg catatcttcc ggaatatcca gcggcaggta
    96661 gagcgacggc aacaccaatt gtaccgcttc atcccacggg ttatcggtgc cgtgaccgta
    96721 ccagatattt gccgcgctga agcggctaac cgaccagcgc aacatgtcct gaatggtttg
    96781 cagctcatta actgcttcat caacgaaaat tttatccacg tattcctcca gggcatgatc
    96841 gcaataattt cggcggctag tttgccatga agatgacgat aaatcagcat tcacgcgcgg
    96901 tgagtgagga aaaatacgtt taaaacgatc gattgcgcta cgagtcgggt aaactgtagg
    96961 aaaattagaa atagagacgc ataaatgaaa aagaaaacaa cacttagcga ggaggaccag
    97021 gctctgtttc gccagttgat ggcggggact cgcaagatta agcaggacac cattgtccat
    97081 cggccacagc gtaaaaaagt cagcgaagtg ccggtgaaac gcttgatcca ggagcaggct
    97141 gatgccagcc attatttctc agatgagttt cagccgttac tgaataccga aggcccagtg
    97201 aaatatgtcc gcccggatgt tagtcatttt gaagcgaaga agttgcgacg cggtgattat
    97261 tcgccggaat tgtttttgga tttacacggt ctgacgcagc tacaggccaa gcaggaactg
    97321 ggggcgttga ttgccgcctg ccgccgtgaa catgtgtttt gcgcttgcgt aatgcacggg
    97381 cacgggaagc atattttaaa acagcaaacg ccgctgtggc tggcacaaca tccgcatgta
    97441 atggcatttc accaggcgcc gaaagagtac ggcggagatg cagcgttgtt agtgttgatt
    97501 gaagtggaag agtggctacc gccggagttg ccatgaatat tagggggcgg atgcgttttg
    97561 ccatttcgcc ctgaatgctg ggaccaaatg ccggatgcgg ggtaaacgcc ttatccggct
    97621 acgaacactg tcgaggctaa taatattaat taaagttcat taagaatata ttgaaagcga
    97681 catgtagcat caaaactttg ccgttcacta gtatcaacac gcacctgcat gtcatggagt
    97741 tgtttatcag ttacaggatg tcggtttgcc gttacgttca ctttattgtt atcacaaccc
    97801 tggacactaa acgtcattaa ttgtgatacc aggcgacaat aggctccacg gtcatcactg
    97861 aagtcactgc catcacataa tcgcccatct ttcattaaga atgtcggcaa cgatttctca
    97921 ataacccccc ccgtagattg caggcttaat gatttattat gaacaacggt gttttcatcc
    97981 caggaaatat catagcttgc tactggctct gttggacaat ttgattggtg cgcattcggc
    98041 ccctgataat aatatggccc cgcctgcgga ggccttgctg tgctataaat tgatgttgtc
    98101 accacccaag ggcaaccatg ggttgtaccc gtaacatgta catttacagg aagtctatga
    98161 caaacccaac cagaggtcat accgggacaa taagaatcat ctactatcgg tgcccgccct
    98221 ggcggaattg ttatgtctgg ggggacaccc ggtccagtcc aggttaagtt aggcacaact
    98281 cctgcctgta ccataatccc cccttgtgaa tatgtcccat aatatgcccc ttcaggggtt
    98341 aacacatcta ttttataaac gatagatgtt gttagcccac tgccataaga tgcgccataa
    98401 gtctgaacat tattaaatgt aacgtctgct tgtacagaag aattaagccc gaacaataac
    98461 ataccaaata aaatcacttt tattttcatt tagatatact caaaaattgc cgtcaggctg
    98521 gcagtaaatt caccagaagt gacttcacgc ccagatttcg cctgcaacca agccttaaag
    98581 ttaagattat tagcaccgtt actcaggtta aatattgccc ctgccgaatt gttgatgggg
    98641 acctgtatcc catcaactgt ttccatgcca atgctcacac cttgcgcacc cgatgatgca
    98701 tccaaagcca gaaaccctgg caaatcgcta tcttcggtac cggttaacgt aactctaact
    98761 ttgtaaccaa caggcccttt gcaatctttc aattgaaaaa cgaccggcac acgatcagat
    98821 tgttcccgtg acattaaatc tctgctgctg atacttggga aatggacttc agccagcact
    98881 tgtccttgta ctaccagggt acaagattta cttaataaat ttccgtagaa gtgtaaatta
    98941 ttgtctgcac gcgctgtgct atttcctgcc agtaacagca ttactatgag tgcgaaccag
    99001 gttttcatct tcattgatat tccacccgta atgtggccga agctgaaaac gttccgtctg
    99061 ttaatacagc atctaccgcc ttcacgggga cagcctctaa ttgtggtttc gtatcggcgt
    99121 taatttgtat cggtgtattt actctaaatg gctgaccatt ttgccgaagt ttaatcccta
    99181 atcctgttac atcagtttcg attgcagcat catcataact tgtttgtgtg cctatccatg
    99241 tcaatgtcat atcccaggaa ctaccgggtg taacagcatc acatttaata tcgtagggca
    99301 cgacctctcg cccattattt ccatcaatgc tatcgataat tacatccctg aactccacct
    99361 cgatagtgtt tccatcacta atagtacatg ccggaggcaa tacaagagtt cctttgaagg
    99421 tgatatccgg tgatgcattc acaataccgc tgctcataca gagcaataaa tacaagagta
    99481 ttttcattga taatccacca ccattgttaa actcccacta aaatcggccc ctgaaagttg
    99541 tgcaccactt tgttttaccg ggactgcatt aagtttcggc tgtgatgtga tattgaaagg
    99601 ggtccagttg ttttctccaa tggagaatag tgagttgtta tctgcattct cgattcgaat
    99661 accaaatcct ggaaccccta catcaacgac tttttctcca ttaattgtgg tggtgtttcc
    99721 tctcaactgc atacgtaaat catcagcttt tcggtcaggg catgacaacg tatatcctgc
    99781 atctttacga taattactac cattgatatc gttgatattc acagtctgaa aatctactga
    99841 actccccgtt acgctacatg gcggaggagg atctacatgt acctgtaaaa tgagatcaac
    99901 aggttttgaa tttgcaagga taatgttata tatcccacta acgcccagca gtaatatacc
    99961 cacccccaca acaaaaattt gtctcatgtc gctctcccgg catcagcctt tgcggctctg
   100021 ctctacatcc acctggcagg tatcaccgcc acatttaaaa tacagcggca tccgtgcgcc
   100081 atagtcgttg acgtaagaca gcaccgggac gctatccatt ttgactttta acggcacaga
   100141 ggtttttggc gatatcacca tgggggaaaa tcccgctgcc ggattgccat ttttctgcgt
   100201 gctggcgtta ctgataacca cgtagtacgg cgtaggatta ttgacggtaa actcctgacc
   100261 gctgcgggtt aaggtcacct tatgctgcca cgggtttttc atgctgacgt tttccagcgc
   100321 ctttggtcgc cagaacagct tgatgcgcgt ttgcagcgct atctgtaacg tgttgggttt
   100381 attcgatttc ggcggaattt cacgcacgtt gaagtagaac acgctctcgc ggtcggcagg
   100441 cagcttgttg atatccggca tcccctgcac cttcacctgg ccgttcatca tggaatcgat
   100501 gcgctgcacc ggcggcagga ccgtcagtgg cgagctaatc ctgttaccct gttcatcttc
   100561 catccagctt tgtgccagat acggcatttt cgggtcgtta ttacgcagcg tgacgctgat
   100621 tgatttatca ctctcattaa aaatcaggcg tgtacggtct ggcgtgacag aggcctgtgc
   100681 tgacaggctg gcagtggtta gtagcaatgc cagcaacatt gtggattttt tggcagagca
   100741 aaatagattc gtcatggatc attccttaat cacgttatta attggcggat accgataccg
   100801 gtgcttccgt tggcatcacc tgctgggtct gctcctggat catcggctta atttcatgag
   100861 gcatcgaagg agatatcgcc cctgtttgct ggcatggtaa taacaggcca ttgaacagat
   100921 cggctggcag cgggtcaggc agatgaatat cgcagtgggc cacaccgccc catgaaacga
   100981 tcatatgctc gccaggtttg acgcccgcga ggtagacgtt gccgtcatcg tcaaccagac
   101041 cgacgttctg cgcgctgtcg tttttcactt ccgcgccaaa cggcggataa ctgccatctt
   101101 gcagacgcag caccgccatc gctttttgcc cgctgatcac cgaaaactta cggtagccaa
   101161 tggctccttc ggtaagcgtc gcctgcacca cggactgggt ggcttcggcg ttttccggca
   101221 ggttgttaag gtcgatatac gcctggttgc ggtagtagtt gttgacgtcg gccaccacgg
   101281 ccttaccgaa catattggtg taaaccgccg caccgtttcc ttccaccgga acaccggcca
   101341 cgccatcggc atcaatcagc aggcgcgtac cgcccatatt ctgggtacgg tggagcgcgc
   101401 caccctgcgc ggtcagcgtc gcgccgccct gtaaggaaat acctgccgag gtgtactgtc
   101461 cttcatgata gttggcgctg agatcgacct gcgccagcga tccgtcgtgg ctgtaataac
   101521 cgtcaacgct ggagtgattg tcgctggtgc ctacgttcaa ctggtaatgg gttgcgtcat
   101581 cgacacggct gaaatacccc acctggctgc tgtccgaacc gctgccgtag ttgccgttat
   101641 agctgatggt gctgctgtca ccccacggca tgctgagcga tatgtaaaca cctttatcgg
   101701 actggttgtc atactcgtag cggtatccgg tcatggaaat gctcatgttg cggatactgc
   101761 ccaggttgaa gtagtgcgag agcatgatgt tgtagttagt ctgttcgtcg cgatcccagt
   101821 aggtatggcg ggtgtagttg agataaacag atacaccggc atccctgaag ttctggttgt
   101881 aggtggcggt gtacatctct ttgtcgttgc cggtgcgcac catttcgctg tcgctggcat
   101941 cgagatactc gctcatggtc atgaagttct cttccgagaa gcggtatccg gcaaaagtga
   102001 cgcggctgtt cagttcatcg aagtctttgg aatagctcac gcgaaacgag ttaccgtcca
   102061 gtgaaccttt accgtaggca gtttctttat ccaggcgggt atgcgagtgg gtgacatcaa
   102121 aggccaccgc accaaacaca gacagatcgc gaccgacgcc aagcgccgcc gactgatagt
   102181 gttcatctgc cagcgccccg ccgtatagcg accagccgtt ggcaatcccc caggaagctt
   102241 cgccgccgga gaagaaaccg ccttccacgt ggtgccccca ctcctgcggg cggcccatca
   102301 tcagtttata gcgcacctgg ccggggcggg tcaggaacgg cattgacgca gtgttaatgt
   102361 catattcctg cacctgaccg ttctgctctt caatgcggat atgcagcgta ccggagacgg
   102421 aatcgccaag atcctggata cggaacggcc ccgccggaac ctgagtttcg taaatcacgc
   102481 ggcccatctg gctgacggtc actttcgcgg tggtgtgcgc cacgccggag atatccggcg
   102541 catagccgcg cagattcggc ggcaacattt gatcgtcggt gctgatactg ccacccacgt
   102601 agttaaagcc gtcgaaaata tcagaattca ggtagtcttc accaaggcca agtttggctt
   102661 ttagcgacgg taaggcccgc caggcgtagt agcggctcca ctcccagttt ttttgcgtgt
   102721 catcaccgtt gataacatcg tcatcattgc ttttactatg caaataatca gtctgccagt
   102781 cggcacgaag acgccatgcg ccaaggttca ccccaaccgt cccgttaccg ctaatttcat
   102841 tactgtcatc cccgccattt tcttcatgtc gtgtctgggc ggtaatactg tagtcagcaa
   102901 ttaaaccaga tataccgtca tcccagcgtg aaggcggatc ccagttgatg tcggtatatt
   102961 caaggtaagc ctggggtaat gaaatgacta acgctgactg actcaggtca gctttaatat
   103021 caatgccttc cagttgaccg ggtttcaggc atttgccgtc gtggatccat tgcaggtttt
   103081 ttgccacatc ctcttttaag ccaaactgcg cgaccagttc aggcgtcagg caggcatagg
   103141 ttttactggc atcgttctca gaggcgtacc agtaaatatc gtattcttcc gtcagcggct
   103201 gtttatttag ttgaacctgt aaattgtatt tcccaggttc gacataaccc tggctggaaa
   103261 atcgcttcag atcgattttt gtgtcgcctt ttaactccag aaaacgggaa tcaaactgaa
   103321 tttcatcttc agcccaggca ttgacataac tcccggacat tgccaatgca acgcagcagg
   103381 gaagtacccg cagtcggaaa agagaatggt caggcataca cgtatccatc cgtaattatt
   103441 ataatttgcg cgaattccat ttaaaaaccc catagcccga aagctatggg ggtatcactt
   103501 agagataagt aatctggaag gtcgtgttgg cttcaaaatt acctaaatct ggcgcatcag
   103561 cagcgcccac cagccaggct ttaaagtcca gcgtctgctt cgctttgcct ttgttggtcg
   103621 ccgtatcgtt tacgattttc tgttcgatac ccgcgccgct tttataagag gttccctgag
   103681 cgtcaccaat ggccaggctg acattgttaa atgccgcacc agtatcggta ttgtaaatgg
   103741 tgtagtaatt gccgctgttg gtagaagacg cgttaccggt gaaggtagtg gtcatcgttg
   103801 tctgggtgtc aaacacacag tcttgcagat ggatctggaa agctttcggt gtagaagtcc
   103861 cgttgttttt caggatccct gaaccaatgt cacccatatc aacttccagt ttgtgatcat
   103921 caggaacgat ggagcacggg gaatcttcaa tggtgccgta gaaacgtgct gtaccattgt
   103981 tgccagcggc gattgttgaa gtagaagcga acgcacccat taccattgtt gcagcaatag
   104041 ctgtttttac aaacttactc attatttgtc cttaaaccat aaataattaa aaatataaac
   104101 attgcaatac
//
LOCUS       DAZFKF010000004        90808 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFKF010000004 DAZFKF010000000
VERSION     DAZFKF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 90808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.004"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            92..676
                     /gene="yraH"
                     /locus_tag="ACHKA1_000346"
     CDS             92..676
                     /gene="yraH"
                     /locus_tag="ACHKA1_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5678052.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            756..1451
                     /gene="yraI"
                     /locus_tag="ACHKA1_000347"
     CDS             756..1451
                     /gene="yraI"
                     /locus_tag="ACHKA1_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5678053.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGLVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1480..3996
                     /gene="yraJ"
                     /locus_tag="ACHKA1_000348"
     CDS             1480..3996
                     /gene="yraJ"
                     /locus_tag="ACHKA1_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5678054.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYSYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            4007..5098
                     /gene="yraK"
                     /locus_tag="ACHKA1_000349"
     CDS             4007..5098
                     /gene="yraK"
                     /locus_tag="ACHKA1_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5678055.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5141..6001)
                     /gene="rsmI"
                     /locus_tag="ACHKA1_000350"
     CDS             complement(5141..6001)
                     /gene="rsmI"
                     /locus_tag="ACHKA1_000350"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO5678056.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            6066..8102
                     /gene="lpoA"
                     /locus_tag="ACHKA1_000351"
     CDS             6066..8102
                     /gene="lpoA"
                     /locus_tag="ACHKA1_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO5678057.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFVGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            8060..8455
                     /gene="yraN"
                     /locus_tag="ACHKA1_000352"
     CDS             8060..8455
                     /gene="yraN"
                     /locus_tag="ACHKA1_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO5678058.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8475..9065
                     /gene="diaA"
                     /locus_tag="ACHKA1_000353"
     CDS             8475..9065
                     /gene="diaA"
                     /locus_tag="ACHKA1_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO5678059.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            9075..9650
                     /gene="dolP"
                     /locus_tag="ACHKA1_000354"
     CDS             9075..9650
                     /gene="dolP"
                     /locus_tag="ACHKA1_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO5678060.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9764..10804)
                     /gene="yraQ"
                     /locus_tag="ACHKA1_000355"
     CDS             complement(9764..10804)
                     /gene="yraQ"
                     /locus_tag="ACHKA1_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO5678061.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10877..11512)
                     /gene="yraR"
                     /locus_tag="ACHKA1_000356"
     CDS             complement(10877..11512)
                     /gene="yraR"
                     /locus_tag="ACHKA1_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO5678062.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11640..12158
                     /gene="yhbO"
                     /locus_tag="ACHKA1_000357"
     CDS             11640..12158
                     /gene="yhbO"
                     /locus_tag="ACHKA1_000357"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO5678063.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12138..12581)
                     /gene="yhbP"
                     /locus_tag="ACHKA1_000358"
     CDS             complement(12138..12581)
                     /gene="yhbP"
                     /locus_tag="ACHKA1_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO5678064.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12632..12934
                     /gene="yhbQ"
                     /locus_tag="ACHKA1_000359"
     CDS             12632..12934
                     /gene="yhbQ"
                     /locus_tag="ACHKA1_000359"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO5678065.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12921..13424)
                     /gene="yhbS"
                     /locus_tag="ACHKA1_000360"
     CDS             complement(12921..13424)
                     /gene="yhbS"
                     /locus_tag="ACHKA1_000360"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5678066.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13418..13942)
                     /gene="ubiT"
                     /locus_tag="ACHKA1_000361"
     CDS             complement(13418..13942)
                     /gene="ubiT"
                     /locus_tag="ACHKA1_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO5678067.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14151..15146
                     /gene="ubiU"
                     /locus_tag="ACHKA1_000362"
     CDS             14151..15146
                     /gene="ubiU"
                     /locus_tag="ACHKA1_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO5678068.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMASNIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15155..16033
                     /gene="ubiV"
                     /locus_tag="ACHKA1_000363"
     CDS             15155..16033
                     /gene="ubiV"
                     /locus_tag="ACHKA1_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO5678069.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16114..17121
                     /gene="yhbW"
                     /locus_tag="ACHKA1_000364"
     CDS             16114..17121
                     /gene="yhbW"
                     /locus_tag="ACHKA1_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO5678070.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17239..18483)
                     /gene="mtr"
                     /locus_tag="ACHKA1_000365"
     CDS             complement(17239..18483)
                     /gene="mtr"
                     /locus_tag="ACHKA1_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO5678071.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18637..20526)
                     /gene="deaD"
                     /locus_tag="ACHKA1_000366"
     CDS             complement(18637..20526)
                     /gene="deaD"
                     /locus_tag="ACHKA1_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO5678072.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20519..20599)
                     /gene="yrbN"
                     /locus_tag="ACHKA1_000367"
     CDS             complement(20519..20599)
                     /gene="yrbN"
                     /locus_tag="ACHKA1_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO5678073.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20706..21590)
                     /gene="nlpI"
                     /locus_tag="ACHKA1_000368"
     CDS             complement(20706..21590)
                     /gene="nlpI"
                     /locus_tag="ACHKA1_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO5678074.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21699..23834)
                     /gene="pnp"
                     /locus_tag="ACHKA1_000369"
     CDS             complement(21699..23834)
                     /gene="pnp"
                     /locus_tag="ACHKA1_000369"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO5678075.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24081..24350)
                     /gene="rpsO"
                     /locus_tag="ACHKA1_000370"
     CDS             complement(24081..24350)
                     /gene="rpsO"
                     /locus_tag="ACHKA1_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO5678076.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24499..25443)
                     /gene="truB"
                     /locus_tag="ACHKA1_000371"
     CDS             complement(24499..25443)
                     /gene="truB"
                     /locus_tag="ACHKA1_000371"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO5678077.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25443..25844)
                     /gene="rbfA"
                     /locus_tag="ACHKA1_000372"
     CDS             complement(25443..25844)
                     /gene="rbfA"
                     /locus_tag="ACHKA1_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO5678078.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(26009..28681)
                     /gene="infB"
                     /locus_tag="ACHKA1_000373"
     CDS             complement(26009..28681)
                     /gene="infB"
                     /locus_tag="ACHKA1_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO5678079.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIESESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28706..30193)
                     /gene="nusA"
                     /locus_tag="ACHKA1_000374"
     CDS             complement(28706..30193)
                     /gene="nusA"
                     /locus_tag="ACHKA1_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO5678080.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30221..30673)
                     /gene="rimP"
                     /locus_tag="ACHKA1_000375"
     CDS             complement(30221..30673)
                     /gene="rimP"
                     /locus_tag="ACHKA1_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO5678081.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30880..30956)
                     /locus_tag="ACHKA1_000376"
     tRNA            complement(30880..30956)
                     /locus_tag="ACHKA1_000376"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30920..30922),aa:Met,seq:cat)
     gene            31304..32647
                     /gene="argG"
                     /locus_tag="ACHKA1_000377"
     CDS             31304..32647
                     /gene="argG"
                     /locus_tag="ACHKA1_000377"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO5678082.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVDNLENKGQ"
     gene            complement(32655..34280)
                     /gene="yhbX"
                     /locus_tag="ACHKA1_000378"
     CDS             complement(32655..34280)
                     /gene="yhbX"
                     /locus_tag="ACHKA1_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5678083.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVAKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKNAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34739..34825)
                     /locus_tag="ACHKA1_000379"
     tRNA            complement(34739..34825)
                     /locus_tag="ACHKA1_000379"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34789..34791),aa:Leu,seq:gag)
     gene            complement(34840..35172)
                     /gene="secG"
                     /locus_tag="ACHKA1_000380"
     CDS             complement(34840..35172)
                     /gene="secG"
                     /locus_tag="ACHKA1_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO5678084.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35400..36737)
                     /gene="glmM"
                     /locus_tag="ACHKA1_000381"
     CDS             complement(35400..36737)
                     /gene="glmM"
                     /locus_tag="ACHKA1_000381"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO5678085.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36730..37578)
                     /gene="folP"
                     /locus_tag="ACHKA1_000382"
     CDS             complement(36730..37578)
                     /gene="folP"
                     /locus_tag="ACHKA1_000382"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO5678086.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37668..39602)
                     /gene="ftsH"
                     /locus_tag="ACHKA1_000383"
     CDS             complement(37668..39602)
                     /gene="ftsH"
                     /locus_tag="ACHKA1_000383"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO5678087.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39702..40331)
                     /gene="rlmE"
                     /locus_tag="ACHKA1_000384"
     CDS             complement(39702..40331)
                     /gene="rlmE"
                     /locus_tag="ACHKA1_000384"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO5678088.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40457..40750
                     /gene="yhbY"
                     /locus_tag="ACHKA1_000385"
     CDS             40457..40750
                     /gene="yhbY"
                     /locus_tag="ACHKA1_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO5678089.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40906..41382)
                     /gene="greA"
                     /locus_tag="ACHKA1_000386"
     CDS             complement(40906..41382)
                     /gene="greA"
                     /locus_tag="ACHKA1_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO5678090.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41630..43063
                     /gene="dacB"
                     /locus_tag="ACHKA1_000387"
     CDS             41630..43063
                     /gene="dacB"
                     /locus_tag="ACHKA1_000387"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO5678091.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHTRFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(43103..44275)
                     /gene="cgtA"
                     /locus_tag="ACHKA1_000388"
     CDS             complement(43103..44275)
                     /gene="cgtA"
                     /locus_tag="ACHKA1_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO5678092.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(44291..45256)
                     /gene="yhbE"
                     /locus_tag="ACHKA1_000389"
     CDS             complement(44291..45256)
                     /gene="yhbE"
                     /locus_tag="ACHKA1_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5678093.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(45383..45640)
                     /gene="rpmA"
                     /locus_tag="ACHKA1_000390"
     CDS             complement(45383..45640)
                     /gene="rpmA"
                     /locus_tag="ACHKA1_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO5678094.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45661..45972)
                     /gene="rplU"
                     /locus_tag="ACHKA1_000391"
     CDS             complement(45661..45972)
                     /gene="rplU"
                     /locus_tag="ACHKA1_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO5678095.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            46231..47202
                     /gene="ispB"
                     /locus_tag="ACHKA1_000392"
     CDS             46231..47202
                     /gene="ispB"
                     /locus_tag="ACHKA1_000392"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO5678096.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPKEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47430..47708
                     /gene="sfsB"
                     /locus_tag="ACHKA1_000393"
     CDS             47430..47708
                     /gene="sfsB"
                     /locus_tag="ACHKA1_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO5678097.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47756..49015)
                     /gene="murA"
                     /locus_tag="ACHKA1_000394"
     CDS             complement(47756..49015)
                     /gene="murA"
                     /locus_tag="ACHKA1_000394"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO5678098.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(49070..49324)
                     /gene="ibaG"
                     /locus_tag="ACHKA1_000395"
     CDS             complement(49070..49324)
                     /gene="ibaG"
                     /locus_tag="ACHKA1_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO5678099.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49484..49777)
                     /gene="mlaB"
                     /locus_tag="ACHKA1_000396"
     CDS             complement(49484..49777)
                     /gene="mlaB"
                     /locus_tag="ACHKA1_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO5678100.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGVTCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49777..50412)
                     /gene="mlaC"
                     /locus_tag="ACHKA1_000397"
     CDS             complement(49777..50412)
                     /gene="mlaC"
                     /locus_tag="ACHKA1_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO5678101.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50431..50982)
                     /gene="mlaD"
                     /locus_tag="ACHKA1_000398"
     CDS             complement(50431..50982)
                     /gene="mlaD"
                     /locus_tag="ACHKA1_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO5678102.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(50987..51769)
                     /gene="mlaE"
                     /locus_tag="ACHKA1_000399"
     CDS             complement(50987..51769)
                     /gene="mlaE"
                     /locus_tag="ACHKA1_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO5678103.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51777..52586)
                     /gene="mlaF"
                     /locus_tag="ACHKA1_000400"
     CDS             complement(51777..52586)
                     /gene="mlaF"
                     /locus_tag="ACHKA1_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO5678104.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAKALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52796..53773
                     /gene="yrbG"
                     /locus_tag="ACHKA1_000401"
     CDS             52796..53773
                     /gene="yrbG"
                     /locus_tag="ACHKA1_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO5678105.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53787..54773
                     /gene="kdsD"
                     /locus_tag="ACHKA1_000402"
     CDS             53787..54773
                     /gene="kdsD"
                     /locus_tag="ACHKA1_000402"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO5678106.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54794..55360
                     /gene="kdsC"
                     /locus_tag="ACHKA1_000403"
     CDS             54794..55360
                     /gene="kdsC"
                     /locus_tag="ACHKA1_000403"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO5678107.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55357..55932
                     /gene="lptC"
                     /locus_tag="ACHKA1_000404"
     CDS             55357..55932
                     /gene="lptC"
                     /locus_tag="ACHKA1_000404"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO5678108.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55901..56458
                     /gene="lptA"
                     /locus_tag="ACHKA1_000405"
     CDS             55901..56458
                     /gene="lptA"
                     /locus_tag="ACHKA1_000405"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO5678109.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56465..57190
                     /gene="lptB"
                     /locus_tag="ACHKA1_000406"
     CDS             56465..57190
                     /gene="lptB"
                     /locus_tag="ACHKA1_000406"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO5678110.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            57238..58671
                     /gene="rpoN"
                     /locus_tag="ACHKA1_000407"
     CDS             57238..58671
                     /gene="rpoN"
                     /locus_tag="ACHKA1_000407"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO5678111.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58694..58981
                     /gene="hpf"
                     /locus_tag="ACHKA1_000408"
     CDS             58694..58981
                     /gene="hpf"
                     /locus_tag="ACHKA1_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO5678112.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            59099..59590
                     /gene="ptsN"
                     /locus_tag="ACHKA1_000409"
     CDS             59099..59590
                     /gene="ptsN"
                     /locus_tag="ACHKA1_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO5678113.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            59636..60490
                     /gene="rapZ"
                     /locus_tag="ACHKA1_000410"
     CDS             59636..60490
                     /gene="rapZ"
                     /locus_tag="ACHKA1_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO5678114.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60487..60759
                     /gene="npr"
                     /locus_tag="ACHKA1_000411"
     CDS             60487..60759
                     /gene="npr"
                     /locus_tag="ACHKA1_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO5678115.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            60973..61605
                     /gene="yrbL"
                     /locus_tag="ACHKA1_000412"
     CDS             60973..61605
                     /gene="yrbL"
                     /locus_tag="ACHKA1_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO5678116.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(61602..62330)
                     /gene="mtgA"
                     /locus_tag="ACHKA1_000413"
     CDS             complement(61602..62330)
                     /gene="mtgA"
                     /locus_tag="ACHKA1_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO5678117.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(62327..62980)
                     /gene="elbB"
                     /locus_tag="ACHKA1_000414"
     CDS             complement(62327..62980)
                     /gene="elbB"
                     /locus_tag="ACHKA1_000414"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO5678118.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGRECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(63210..65546)
                     /gene="arcB"
                     /locus_tag="ACHKA1_000415"
     CDS             complement(63210..65546)
                     /gene="arcB"
                     /locus_tag="ACHKA1_000415"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO5678119.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65642..66571)
                     /gene="yhcC"
                     /locus_tag="ACHKA1_000416"
     CDS             complement(65642..66571)
                     /gene="yhcC"
                     /locus_tag="ACHKA1_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO5678120.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            67246..71706
                     /gene="gltB"
                     /locus_tag="ACHKA1_000417"
     CDS             67246..71706
                     /gene="gltB"
                     /locus_tag="ACHKA1_000417"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO5678121.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIIETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTET
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSAFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71719..73137
                     /gene="gltD"
                     /locus_tag="ACHKA1_000418"
     CDS             71719..73137
                     /gene="gltD"
                     /locus_tag="ACHKA1_000418"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO5678122.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TNDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            73321..74448
                     /gene="yhcG"
                     /locus_tag="ACHKA1_000419"
     CDS             73321..74448
                     /gene="yhcG"
                     /locus_tag="ACHKA1_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFO5678123.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSLYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPAETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGVGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRLR"
     gene            complement(74508..74972)
                     /gene="nanQ"
                     /locus_tag="ACHKA1_000420"
     CDS             complement(74508..74972)
                     /gene="nanQ"
                     /locus_tag="ACHKA1_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO5678124.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(74969..75844)
                     /gene="nanK"
                     /locus_tag="ACHKA1_000421"
     CDS             complement(74969..75844)
                     /gene="nanK"
                     /locus_tag="ACHKA1_000421"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO5678125.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(75841..76530)
                     /gene="nanE"
                     /locus_tag="ACHKA1_000422"
     CDS             complement(75841..76530)
                     /gene="nanE"
                     /locus_tag="ACHKA1_000422"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO5678126.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVEMLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(76578..78068)
                     /gene="nanT"
                     /locus_tag="ACHKA1_000423"
     CDS             complement(76578..78068)
                     /gene="nanT"
                     /locus_tag="ACHKA1_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO5678127.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(78177..79070)
                     /gene="nanA"
                     /locus_tag="ACHKA1_000424"
     CDS             complement(78177..79070)
                     /gene="nanA"
                     /locus_tag="ACHKA1_000424"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO5678128.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(79192..79983)
                     /gene="nanR"
                     /locus_tag="ACHKA1_000425"
     CDS             complement(79192..79983)
                     /gene="nanR"
                     /locus_tag="ACHKA1_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO5678129.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            80363..81730
                     /gene="dcuC"
                     /locus_tag="ACHKA1_000426"
     CDS             80363..81730
                     /gene="dcuC"
                     /locus_tag="ACHKA1_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO5678130.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPTKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHKLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(81772..82269)
                     /gene="sspB"
                     /locus_tag="ACHKA1_000427"
     CDS             complement(81772..82269)
                     /gene="sspB"
                     /locus_tag="ACHKA1_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO5678131.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(82275..82913)
                     /gene="sspA"
                     /locus_tag="ACHKA1_000428"
     CDS             complement(82275..82913)
                     /gene="sspA"
                     /locus_tag="ACHKA1_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO5678132.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(83308..83700)
                     /gene="rpsI"
                     /locus_tag="ACHKA1_000429"
     CDS             complement(83308..83700)
                     /gene="rpsI"
                     /locus_tag="ACHKA1_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO5678133.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(83716..84144)
                     /gene="rplM"
                     /locus_tag="ACHKA1_000430"
     CDS             complement(83716..84144)
                     /gene="rplM"
                     /locus_tag="ACHKA1_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO5678134.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(84363..85490)
                     /gene="zapE"
                     /locus_tag="ACHKA1_000431"
     CDS             complement(84363..85490)
                     /gene="zapE"
                     /locus_tag="ACHKA1_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO5678135.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDEIRAQMDKLWLALAGAKREISPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHAVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            85684..86082
                     /gene="zapG"
                     /locus_tag="ACHKA1_000432"
     CDS             85684..86082
                     /gene="zapG"
                     /locus_tag="ACHKA1_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO5678136.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            86236..87603
                     /gene="degQ"
                     /locus_tag="ACHKA1_000433"
     CDS             86236..87603
                     /gene="degQ"
                     /locus_tag="ACHKA1_000433"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO5678137.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            87693..88760
                     /gene="degS"
                     /locus_tag="ACHKA1_000434"
     CDS             87693..88760
                     /gene="degS"
                     /locus_tag="ACHKA1_000434"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO5678138.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(88823..89761)
                     /gene="mdh"
                     /locus_tag="ACHKA1_000435"
     CDS             complement(88823..89761)
                     /gene="mdh"
                     /locus_tag="ACHKA1_000435"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO5678139.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            90196..90666
                     /gene="argR"
                     /locus_tag="ACHKA1_000436"
     CDS             90196..90666
                     /gene="argR"
                     /locus_tag="ACHKA1_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO5678140.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT        22396 a        24386 c        22529 g        21497 t
ORIGIN      
        1 gaaatatttc tacctgtata atctttaaag atgttgaaca tatattcaca ttaaatatga
       61 ttatgtactt gttacaagga taaggttata aatgaataaa gttacaaaaa cagctattgc
      121 gggcttactt gcgcttttcg caggtaatgc cgctgcaacc gatggtgaaa tagtttttga
      181 tggcgagatt ttaaaatccg cttgtgaaat caatgactct gataagaaaa ttgaagttgc
      241 tcttggtcac tataatgccg aacagtttcg ttctgtaggc gatcgtagcc caaaaattcc
      301 attcactatt cctttagtta actgcccagt gactgggtgg gagcacgata acggcaacgt
      361 tgaagcgtct ttccgtttat ggctggaaac gcgggataat ggtaccgtcc ctaacttccc
      421 taacttggca aaagtgggct cttttgctgg cacagcagca acaggtgtgg gtatccgtat
      481 tgacgatgcg gaaagcggaa accttatgcc actaaatgct atgggcaatg ataacacagt
      541 ttatcagatc cctgcggact ccgctggaat tgtcaatgtt gacctcatcg cttactacgt
      601 atcaaccgtt gaagcatccg aaatcacccc aggggaagca gacgctgtcg ttaacgtaac
      661 gctggattac cgttaatacg ttacggcgtt atctgacctt tcagataacg cccttttcct
      721 tcctctttct cgttgtatca ggttgaaaaa taactatgtc aaaacgaaca ttcgcggtga
      781 taataacctt gttgtgtagc ttctgtatcg gccaggcgct tgcagggggt atcgttttac
      841 agcgaacgcg agtgatctat gatgccagcc gcaaagaggc tgcgttacct gtcgcaaaca
      901 aaggcgcaga aacgccttat ttactgcaat catgggtaga taatatagat ggtacaagcc
      961 gtgccccatt tattataacc ccaccgctat ttcgtcttga agctggcgat gattcatcac
     1021 tgcgaattat taaaacagct gataacctgc ctgaaaataa agagtcgctg ttctacatta
     1081 atgttcgagc cattccagca aagaaaaaat cagatgatgt taatgctaac gagttgacgc
     1141 tggtatttaa aacacggatc aaaatgtttt atcgccccgc acacttgaag gggttggtca
     1201 acgatgcgtg gaaatcactg gaatttaaac gtagtgacca ttcactcaat atatataacc
     1261 caactgaata ttacgtcgta tttgccggac tggcagtcga taaagccgat ctcacaagca
     1321 aaattgaata tatcgcgccc ggagaacata aacagttacc acttccagca tctggcggaa
     1381 agaacgtgaa atgggctgcg atcaatgatt atggcggcag ttccgggaca gaaactcgtc
     1441 cactgcaata acaaatataa aaacacaggt catcagggaa tgccacaacg acaccaccag
     1501 ggacataaac gcacaccgaa acagttggcg ctcattatca aacgctgttt gccgatggtg
     1561 ctcactggca gcggcatgct ttgcactacc gctaacgccg aagagtatta tttcgacccc
     1621 attatgctgg aaaccacaaa aagtggtatg caaacaaccg atctgtcacg tttttcaaaa
     1681 aaatacgcac aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt
     1741 tcacagaaaa aaattacatt taccgccaat gcagagcaac ttctgcagcc acagtttacg
     1801 gtagaacaac tacgtgagct gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa
     1861 gatgacgata gcgtgatcaa ctcgcttgaa caaatcattc ccggtacagc tgctgaattt
     1921 gatttcaatc atcagcgact taatttgagc attccccaaa ttgcactgta ccgtgatgca
     1981 agaggttacg tctccccttc tcgttgggac gatggtatac caacgctgtt taccaactac
     2041 tcgtttacag gttctgataa ccgttaccgc cagggcaatc gtagccaacg acagtacctg
     2101 aatatgcaaa atggtgctaa ttttggcccc tggcgattac gtaactattc tacgtggaca
     2161 cgcaacgatc aggcgtcaag ctggaacact atcagtagtt atttacaacg tgatatcaag
     2221 gcgttgaagt ctcagttgct tctgggagaa agcgccacca gcggcagtat tttttccagc
     2281 tacaccttta ctggcgtgca actcgcttcc gacgataata tgttgccaaa cagccagcgc
     2341 ggatttgccc caacggtacg cggtatcgca aacagtagtg caatcgtgac tatcaggcaa
     2401 aatggttatg tgatctatca aagcaacgtg ccagcgggtg cctttgaaat taacgatctc
     2461 tacccctctt ccaacagcgg cgatttagaa gtcacgattg aagaaagtga cggtacacaa
     2521 cgtcgcttta tccagcctta ttcttcatta cccatgatgc agcgacctgg gcatctaaaa
     2581 tatagcgcga ccgctggacg ctatcgcgct gatgcaaaca gtgatagcaa ggaacccgaa
     2641 tttgctgaag ccacggcaat atatggtttg aataatactt ttacgctgta tggcggcctg
     2701 ctcggttctg aagattatta tgcgctgggg atcggtatcg gcggcacact tggcgcactg
     2761 ggcgcgttgt cgatggatat caacagagct gacacccaat tcgataacca gcactctttt
     2821 catggctatc aatggcgtac gcagtacatc aaagatatcc cggaaaccaa caccaatatc
     2881 gctgtcagct actatagcta taccaacgat ggctatttta gttttgatga agccaatacc
     2941 cgcaattggg actataacag tcgccaaaaa agtgaaattc aattcaacat cagccagaca
     3001 atatttgatg gggtaagtct gtatgcctcc ggttcacagc aagactattg gggcaataac
     3061 gagaaaaaca ggaatatctc tgttggggtt tccggccagc aatggggaat tggttacagc
     3121 ctgaattatc aatacagccg ctacactgat caaaataatg accgcgcact ctctttgaat
     3181 ctcagtattc cgttagaacg ctggttaccg cgtagccggg tttcctatca gatgaccagc
     3241 cagaaagatc gcccaaccca acatgaaatg cgtcttgatg gctcactgct ggatgatggt
     3301 cgcctgagct atagcctgga acaaagtctg gatgacgata acaaccataa cagtagcctg
     3361 aacgccagtt accgttcacc ttatggaacc ttcagtgccg gatacagtta cggtaatgac
     3421 agcagccaat acaattacgg cgttaccggc ggcgtggtta tccatcctca tggcgtgacg
     3481 ctctcgcaat atctgggcaa cgcttttgcg cttatcgatg ctaacggggc atctggcgtg
     3541 aggatacaaa actatccggg gattgctacc gatccctttg gctatgcagt ggttccttat
     3601 ctcacaactt atcaggaaaa ccgtctctcg gtagatacta cgcagctgcc cgataacgtc
     3661 gatcttgaac aaacaacaca gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt
     3721 ttcaacgcca atatcggtta tcgcgtactt gttacagtca gcgatcgcaa cggtaaaccg
     3781 ttgccctttg gcgctcttgc cagcaacgat gatacggggc aacaaagtat cgtcgatgag
     3841 ggcggcatac tatatctctc tgggatatcg agtaaatcac aaagctggac tgtacgctgg
     3901 ggaaatcagg cagatcaaca atgtcagttt gcttttagta caccggattc agaaccaaca
     3961 acctctgtat tacaaggcac agcgcagtgc cattaaggat aaaaaaatga aaagagcgcc
     4021 tcttataaca ggacttttgt tgatatccac atcctgcgct tatgcctcct caggagggtg
     4081 tggagccgac agcactagcg gtgcgacaaa ttacagcagt gtggttgatg atgttacggt
     4141 gaaccagaca gataacgtga caggacggga gtttacctct gcaacgctaa gtagcactaa
     4201 ctggcaatac gcctgttcct gctctgcggg taaggcagtt aaacttgtct atatggtcag
     4261 ccccgtactt accaccactg gacatcagac aggatattac aaactcaatg acagcctgga
     4321 tattaaaacc acattacagg caaacgacat tccaggactc acaaccgacc aggttgtctc
     4381 tgttaacacc cgattcacac agataaaaag cagcaccgta tattctgctg caacccaaac
     4441 gggtgtttgc cagggtgaca cgtctcgtta tggacccgtt aatattggtg caaataccac
     4501 ctttaccctg tatgtcacca agccatttct cggctcgatg accattccga aaacggatat
     4561 tgccgtcatt aaaggcgcgt gggtcgatgg aatgggaagc ccgtctacag gtgacttcca
     4621 tgatttagtc aagttatcga tacagggaaa tctcaccgcc ccacagtcgt gcaaaattaa
     4681 tcagggcgat gttattaagg ttaattttgg attcatcaat ggtcagaagt ttaccacccg
     4741 caatgccatg ccagacggtt ttactccagt agactttgat atcacttatg actgtggtga
     4801 tacttcaaag attaaaaact cgttgcaaat gcgcatcgac ggtacaactg gggtagtaga
     4861 ccagtacaac ctggtcgcca gacgaagaag ttcagacaat gcgcccgatg tcggtattcg
     4921 tattgaaaat ctcggtggcg gagttgcaaa tattcctttt cagaacggta tccttcccgt
     4981 tgatccttcc gggcatggca ccgtcaatat gcgcgcctgg ccagttaatc tggtcggtgg
     5041 tgagctggaa acaggaaaat ttcagggcac agccaccatt accgtcatcg tgcggtaaaa
     5101 gaagacgttg cgttttgatt aacgcaacgc ttcggcactc ttacccctgc tgctccagcg
     5161 catacttata tagcgcattt ttcttcacgc cgtgaatttc tgcggccagc gccgccgctt
     5221 ttttcagcgg cagttctgcc tgtagcagcg ccagcgtgcg cagggcatcg gcgggtaagt
     5281 cttcttcctg tgctttatga ccttcgacaa tcagcaccat ttcgcctttg cgacggtttt
     5341 catcttcctt tacccacgcc agcagctcgc caacgggcgc gccgtgaatg gtttcccagg
     5401 ttttggtcag ctcacgcgcc agaaccacgt agcgggattc gcctaatacc gcaacgatat
     5461 cttccaggct atctaacaga cggtgggtag attcataaaa aatcagcgtg cgcggctccg
     5521 cttcaatggc ttttagcgca tcacggcggc cttttgattt ggcaggtaaa aagccttcgt
     5581 aacagaaacg gtcagagggt aaacccgcgg cgcttaacgc agtgatagca gcacacggtc
     5641 cgggtagcgg caccacacga atccccgctt cacggcaggt acgcaccaga tggtagccag
     5701 gatcgttaat tagcggcgtt ccggcatcgg aaaccagcgc aatgttttgc ccctcttgca
     5761 gcttcgccag cagcgtttcg gctttttgtt gttcgttatg gtcgtgcagc gcaaacaacc
     5821 gggcattaat cccaaaatgt tgcagcaata aaccggtgtg acgagtatcc tcggcggcaa
     5881 tcagatcaac ggcctgtaat acctctaacg cacgctgggt gatatccgcc agattgccga
     5941 ttggcgtcgg tacaatgtaa agctggccct gagaattatc cgccgattgg tgttgtttca
     6001 ttgtgtcgtc cgtattgccg atttaatatt gagcattgcg taaaaaaaat atcactggat
     6061 acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg cctgttgttc
     6121 tggcagccct gattttcgtc ggttgtggca cccatactcc cgatcagtcc actgcttata
     6181 tgcagggcac ggcgcaggct gattctgcct tttatcttca gcagatgcag caaagctctg
     6241 atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa gaaggtaaaa
     6301 ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat tctcagcgtc
     6361 gcgagaaaac actgctggcg gtagagatta aactggcgca gaaagatttt gctggcgcgc
     6421 aaaacttgct ggcgaaaatt acacccgccg atttagaaca aaaccagcaa gcccgttact
     6481 ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg cgcgcgttaa
     6541 ttgctcagga accgctgctt ggcgcgaaag aaaaacagca gaatattgat gccacctggc
     6601 aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac gccgacgaaa
     6661 atattctgca aggctggctg gatctgcagc gcgtctggtt tgataaccgt aacgatcccg
     6721 acatgatgaa agccgggatc gccgactggc agaaacgtta tccgaacaac ccgggcgcga
     6781 aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg accaacaaaa
     6841 tcgccctgct gttgccactg aatggccagg cagcggtatt tggtcgcact attcagcaag
     6901 gctttgaagc ggcgaaaaat atcggcactc agccagtggc agctcaggta gctgccgcac
     6961 ctgccgcaga cgtagcagaa caaccccaac cgcaaaccgt ggatggcgtt gccagcccgg
     7021 cacaagcctc ggttagcgat ctgaccggtg aacagcctgc agcccagccg gtgcctgtaa
     7081 gcgccccggc gacaagcacc gcagcggtaa gcgcacccgc aaatccatcc gcagagctga
     7141 aaatctacga tacctcatca caaccactta gccagatctt aagccaggtt cagcaggatg
     7201 gcgcgagtat tgtggtcggt ccgttgctga aaaataacgt tgaagagttg ctgaagagca
     7261 acactccgct gaacgtactg gcactgaacc agccggagaa tatcgaaaat cgcgtcaata
     7321 tttgttactt cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt catattcgtg
     7381 accagggtaa acaagcgccg ctggtgctga tcccacgcag ttcattgggc gatcgcgtag
     7441 ccaatgcgtt tgcgcaagag tggcagaaac tgggcggcgg caccgttctg caacaaaaat
     7501 ttggttccac cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatc gctttaacgg
     7561 gtaccccgat tactcccaga gcgacaaccg actccggcat gacgaccaac aatccaacgc
     7621 tgcaaaccac gccaaccgat gaccagttca ccaataatgg cggtcgtgtc gatgcggtgt
     7681 acattgtggc aacgccgggt gaaatcgctt ttatcaaacc gatgatcgcc atgcgtaacg
     7741 gtagccagag cggtgcaacg ctgtacgcca gctcccgcag tgcgcaaggg accgctggcc
     7801 cggatttccg actggagatg gaaggcttgc agtacagcga aatcccgatg ctggcaggcg
     7861 gtaatctacc gttaatgcag caggcactca gcgcggtgaa taacgattat tcactggctc
     7921 gcatgtatgc gatgggcgtc gatgcctggt cgctggcaaa tcatttctca caaatgcgcc
     7981 aggttcaggg ttttgaaatc aacggtaata ccggaagcct gacggctaac ccggattgcg
     8041 tgattaacag gaagttatca tggctacagt accaacaagg tcaggtagtc cccgccagtt
     8101 aaccaccaaa cagaccggcg atgcgtggga agtacaagcg cgtcgctggc tggaaggcaa
     8161 aggactgcga tttgtcgccg ctaacgtgaa cgagcgtggc ggcgagatcg atctgataat
     8221 gcgtgaaggc cggaccaccg tttttgtcga ggtgcgttac cgccgttctg cgctttatgg
     8281 cggcgcggca gccagtgtga cccgcagcaa acaacacaaa ttattacaga ctgcccgctt
     8341 gtggctcgcg cgtcataatg ggagttttga tactgtggat tgccggttcg atgtggtagc
     8401 cttcaccggg aatgaggttg agtggattaa ggatgccttt aatgaccact cataattaag
     8461 gtttaaggat tagcgtgcaa gaaagaatta aagcttgctt cactgaaagc attcaaactc
     8521 aaattgcggc ggcagaggcg cttccggatg ccatctcccg tgcggccatg acgctggttc
     8581 agtctctgct caatggcaac aaaatcctct gttgtggtaa tggaacttcc gctgccaatg
     8641 cacagcattt tgctgccagc atgatcaacc gtttcgaaac ggagcggccc agcttacctg
     8701 ccattgcact aaatactgat aatgttgtct taacggcgat tgccaacgat cgcttacatg
     8761 atgaagtgta tgcaaaacag gtgcgggcgc tgggtcatgc gggagatgta ttgttagcca
     8821 tttccacccg tggcaacagc cgcgatattg taaaagcggt agaagctgcc gttacgcgtg
     8881 atatgaccat tgtggcattg accggctatg acggcggtga acttgcaggt ttgttagggc
     8941 cacaggatgt ggagatccgc atcccttcgc atcgcagtgc tcgcattcag gaaatgcata
     9001 tgctgacggt aaattgcctg tgcgatctga tcgataacac gcttttccct caccaggatg
     9061 attaaggaga atacatgaag gcattatcgc caatcgcagt ccttatttcc gcgctgctgt
     9121 tgcaaggttg tgttgccgct gccgtagtgg gtaccgctgc tgtgggtacc aaagccgcaa
     9181 ctgacccacg cagtgtcggc acccaggtgg acgatggtac cctggaagtg cgcgtgaaca
     9241 gcgcattgtc gaaagacgaa cagattaaga aagaagcgcg cattaatgta acggcctatc
     9301 agggcaaagt gctgctggtt gggcagtcac caaacgctga actttcggct cgcgccaaac
     9361 agattgctat gggcgtagac ggtgccaacg aagtgtataa cgagattcgt cagggccagc
     9421 cgattggtct gggcgaagcg tctaatgata cgtggatcac caccaaagtg cgttcgcagc
     9481 tcttaaccag cgacctggtg aaatcgtcca acgtgaaagt gaccaccgaa aacggtgaag
     9541 tgttcctgat ggggctggtg actgaacgtg aagcgaaagc ggcggcagat attgccagcc
     9601 gggtgagcgg cgtgaagcgg gtaactacgg cgtttacgtt tattaaatag cagccccttg
     9661 taatgcctga tgcgacgctt gccgcgtctt atcaggccta caacgatgca aaccgtaggt
     9721 cggataaggc gtttacgccg catccgacat gttttccctc aaatcagaat aacagcgcca
     9781 gcccgccgac aatcacgcct gacactgcca ccatcgcccc cgtcagccat aaggctttcg
     9841 ccgggaacgc tttgcgcagc ataatcagtg acggcaaact caccgccggg agcgtcatca
     9901 acaatgccag cgccggagca gttcccatac ctgccagcat catcgtttgt acaatcggaa
     9961 tttctgctgc cgtgggaatg acaaacaagc atcctgctac cgccatcgcc accacccaca
    10021 tcaggctgtt atcgacagca ccatcggcat gggggaataa ccagacgcga gcggcaccca
    10081 acaccagtac tgcaaggatg taaaccggga tcgtactcca gaaaagcgtc catagcgccc
    10141 tgccccagcg gctaaaaaat ccgccctgtg cttccggtat gtcaatttcg accggtgcct
    10201 gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt cgcaatcagc aacaccatca
    10261 ccagcccggc caccagacga atcgccgcaa aaccccagcc gaggacaaac cccataaaca
    10321 ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca ccgcccatcg
    10381 acacctgttg gcgacgcatt cccgccgcga ccggagccgc acagcaggta cacatcatgc
    10441 ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc ccaagcgtac
    10501 gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc acccccagca
    10561 ctgccgcttt ccataccgcg aggaaataga tcatcgcgta atccaacgcc gcttgccacg
    10621 gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg gcagcagtaa
    10681 acgctttgcc gtagtaaggt tgccatttca cataccagag accggcaatg acaacgagaa
    10741 agaaaagcgc gggtttccac cactgaatgg gcgttgccgc ctgagatgaa gactgaccag
    10801 tcatagcatt ccccaggaat aagttatgat aattgagcgc gtgaatatta cgctcactat
    10861 caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt aaaatagtca
    10921 cgccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg tctctcgcat
    10981 caatggattt ccagttaccg ggtaacaaac ggaacagcgg cgcaaaaagc gtttcgttca
    11041 tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc aatttcggcc
    11101 agttctgggc aattaatgct tcttccatct cccctttgac gcggttatag aaaaacggcg
    11161 agtgggcatt ggcacccata gcactgacca ccagcatatg ctgcgcgccc agccgccgcc
    11221 cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc tctttgctcc
    11281 ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatggga tcggtgacct
    11341 gcgccagcgc gtcagtcagt tgcggatcat ggggattaaa caccccgggc atatcgccca
    11401 acggacgtcg cgtcggcgcg gctattgcgt taactttcgg ttcgttaatc aacatccgca
    11461 gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc atcttcgctc
    11521 ctttacagaa ttgtctacct tgcgctccac ggctcatgca ccaggcttaa tagaccatga
    11581 ggtaattatc cccgattgtg ggaaattcgc ctcatccaat gcaacaacgc ggaggaagca
    11641 tgagtaagaa aattgccgtt ttaatcactg atgaatttga agattcagaa tttacttcac
    11701 ccgcagacga gttccgtaaa gccggacacg aagtgattac cattgaaaaa caagcgggta
    11761 aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc atcgatgaag
    11821 tgacgccagc ggagtttgat gccctgttgc taccgggcgg tcattcaccg gattatctgc
    11881 gtggcgacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc aaacctgttt
    11941 tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg gggcgcaaac
    12001 tgaccgcagt taaaccgatc attattgatg ttaaaaatgc gggcgcggaa ttttacgatc
    12061 aggaagtcgt ggttgataaa gatcagctgg ttaccagccg gacgccggac gatctgccag
    12121 cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagc cgcgtagcca aatcattttt
    12181 ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat ttcccacacc
    12241 ggggccgaca gcattctggc aaccggaaag cgacgattgt acgccttgcg cgcgaggtcg
    12301 ctttcctcac cttccagcct gcggatctca cctttaaact gcacgccgcg aattaacgct
    12361 accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc actcatctgc
    12421 gcatgacgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc atcaaacaga
    12481 taaaaagcat tagcgcacca gagttcccct tcctgctgca cacaccaggt gacaacatgt
    12541 tgttttgcca accaacggct gatggtgatg agtgtttcca ttgctgttct cttttatact
    12601 gtgggcctga ccttaacatg cgacgcagac gatgacaccc tggtttcttt acttgatccg
    12661 taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc gctatcagca
    12721 gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga cgctggcgtt
    12781 ttctgcgcca gtgggcgatc gttcgctggc gttacgggcg gaatatcgcg ttaaacaact
    12841 gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca gggtttgcag agctgttaag
    12901 cagtctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc aaccagacca
    12961 gttacgccat tcaacgcgtc atccgctaaa cgatgtacct ggaaggcgct ttcagtgcct
    13021 ggccagcggc aacgcagatc gtgatgtgcc gccagttcaa agccaaaacg gctgtacagc
    13081 gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga atcgagtcct
    13141 tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatctac cgccaacggt
    13201 gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa tgccacatag
    13261 ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa gccatcttca
    13321 cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg cagcagggca
    13381 tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca cctaccgatg
    13441 tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc agttgcagca
    13501 gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc aggtttttca
    13561 catacagccc cagctccgta tcgccttcaa tcaccagccg acgctggaag aagagcgtat
    13621 ccggatcctg tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca ctaaaactca
    13681 catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag gtaaaccatt
    13741 gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaac tccagctcgc
    13801 catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc tggcgtttta
    13861 gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa tgcacaatac
    13921 gggaacgcag tttatccaac acgagtttta ctccctgttt caacaatcat cctattttgc
    13981 catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat tgctgctacc
    14041 accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc
    14101 cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt atggagctgc
    14161 tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc gcagatgctg
    14221 tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt aactttaccg
    14281 agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa cttcacatcg
    14341 cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgtgcc gtggatatgg
    14401 cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgctg gagtacgccg
    14461 ccgagcgtta cccgcatatt gagcgtcatg tgtcagtgca ggcttcggcg accaatgaag
    14521 aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg ccgcgcgtgt
    14581 tgtcgattca tcaggtgaaa cagctggcac gggtcacacc tgtcccgctg gaagtgtttg
    14641 ctttcggcag cctgtgcatt atgtcggaag gtcgttgcta tctgtcgtcg tatctgacgg
    14701 gtgagtcgcc caacaccgtg ggcgcgtgtt ctccggcccg tttcgtgcgc tggcaacaaa
    14761 cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgatcgttat caggacggcg
    14821 aaaacgcagg ttatccgacg ttgtgtaaag ggcgttatct ggtggacggc gagcgctatc
    14881 acgcgctgga agaaccaacc agtctcaata ccctggaact gctaccggag ttaatggcgt
    14941 cgaatatcgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat gtcagccagg
    15001 tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa aacttcgtac
    15061 cgcaaagcgc gtggatggag acgctcgggt cgatgtccga aggcacgcaa accacccttg
    15121 gcgcgtatca ccgtaaatgg cagtgagaag agcaatgaaa tattccttag ggccagtgct
    15181 gtggtactgg ccaaaagaga cgctggaaga tttttatcag caggccgcca ccagcagcgc
    15241 cgacgtgatt tatcttggcg aagcggtatg cagcaagcgc cgggcaacca aagttggcga
    15301 ctggctggag atggcaaaat cgctcgccgg aagtggtaag cagatagtgc tctccacgct
    15361 ggcgctggtg caggcatcat ctgaactggg cgaactgaaa cgctatgttg agaacggtga
    15421 gtttctgatt gaagccagcg atctcggcgt ggtgaatatg tgcgctgaac gcaaactgcc
    15481 gttcgtcgcc ggacacgcgc tgaactgcta taacgcggtg acgctgaaaa tattgctcaa
    15541 acagggcatg atgcgctggt gtatgccggt ggagctttcc cgcgactggc tggtgaatct
    15601 gcttaatcag tgcgatgagc tgggcattcg caaccagttt gaagtggaag tcctgagcta
    15661 cggtcatcta ccgctggcct actccgctcg ctgctttacc gcgcgttcgg aagaccgccc
    15721 gaaagatgag tgtgaaacct gctgcattaa gtatccgaac gggcgcaacg tgctgtcgca
    15781 ggaaaaccaa caagtgtttg tactcaatgg cattcagacc atgagcggct acgtttacaa
    15841 cctcggtaac gagctggcat ccatgcaggg cctggttgat gtggtccgcc tgtcaccgca
    15901 gggtactgac actttcgcga tgctcgacgc cttccgcgct aatgaaaatg gcgctgcacc
    15961 actgccgttg acggcgaaca gcgactgtaa cggctactgg cggcggctgg caggactgga
    16021 gctgcaagcc taagtaaata gctcactttg ttaacaactt taactactct ttaatgcagt
    16081 attaaagatt aatcggtaac aaagtgagct gttatgactg ataaaaccat tgcgttttcg
    16141 ctactcgatc tggcccccat tcccgaaggt tcttcagcgc gagaagcatt ctcccactct
    16201 ctcgatctcg cccgtctggc tgaaaagcgc ggctatcatc gctactggct ggcagaacac
    16261 cacaatatga ctggcattgc cagtgctgcc acgtcggtat tgattggcta tctggcggcg
    16321 aataccacca cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca ctcaccgttg
    16381 gtcattgcag aacagttcgg cacgctcaat acactctatc cggggcgaat cgatttgggg
    16441 ctgggtcgtg ctccgggtag tgaccagcga accatgatgg cgctacgtcg tcatatgagc
    16501 ggcgatattg ataatttccc ccgggatgtc gcggagctgg tggactggtt tgacgcccgc
    16561 gatcccaatc cgcatgtgcg cccggtacca ggctatggcg agaaaatccc cgtgtggttg
    16621 ttaggctcca gcctttacag cgcgcaactg gcggcgcagc ttggtctgcc gtttgcgttt
    16681 gcctcacact tcgcgccgga tatgctgttc caggcgctgc atctttatcg cagcaacttc
    16741 aaaccgtcag cacggctgga aaaaccatac gcgatggtgt gcatcaatat tatcgccgcc
    16801 gacagcaacc gcgacgctga atttctgttt acctcaatgc agcaagcctt tgtgaagctg
    16861 cgccgtggcg aaaccgggca actgccgccg ccgattcaaa atatggatca gttctggtca
    16921 ccgtctgagc agtatggcgt gcagcaggcg ctgagtatgt cgttggtagg tgataaagcg
    16981 aaagtgcgtc atggcttgca gtcgatcctg cgcgaaaccg acgccgatga gattatggtc
    17041 aacgggcaga ttttcgacca ccaggcgcgg ctgcattcgt ttgagctggc gatggatgtt
    17101 aaggaagagt tgttgggata gtgtgtctta acgcgggaag ccttatccga gctggcaacg
    17161 ctgtcctaca tagacctgat aagcgaagcg catcaggcat tgtgtaggca gcagaaatgt
    17221 cggataaggc accgctgatt actgatacac cggcagtaaa ttaaagctcg ataaaatatg
    17281 caccagtgcg ttgccgacgc caaacaccag aatcagcgca atcatcggct tgccacccca
    17341 gacgcggaat ttcgggctgc caaagcgttt acgcgatgcg cgggccagca gtgccggaac
    17401 aattgccgcc cagatggtag ccgctaaacc agcataacca atggcgtaca ggaatccgtt
    17461 cgggaacagc aggcccccca cgacaggcgg ggcaaaggtc agcaatgctg ttttcaagcg
    17521 gcccacagct gagtcatcga aaccaaacag atctgccaga tagtcaaaca aacccagcgt
    17581 tacgccaagg aacgaactcg ctaccgcaaa gtttgagaac acgaccagca gcagatccag
    17641 actacggctg ttcagtacgc cgcttaacgc ctgtaccagc acatcaatat taccgccctt
    17701 ctctgcaata ccgataaact ccggacgcgg gatgttaccc atcgtcgcca gcaaccagat
    17761 ggtatacagc gccagcgcca tcagcgtacc gtacaccagg catttcacga tggttttcgg
    17821 atctttgccg taatacttca tcaggcttgg cacgttaccg tgataaccaa acgatgccag
    17881 acagaacggc agggtcatca acagatacgg tgcataagac gcattgcttt cggcgacgtt
    17941 gaacaatgtc gcaggctgca catgccccag caggctacca aaggtgagga agaaggtaat
    18001 gactttcgcc cccagcacaa tcgctgtcat gcgactgacg gctttagtgc tcaaccacac
    18061 cacaaacgct accaacaatg caaaaccaaa acccgccgcc cgtgccggga cgtttagtga
    18121 catctctgcg aaggtgtgat gcagaatcga accactggca gaaatatagg cataggtcag
    18181 gatatagagc acaaaggcaa tggaaatgcc gttgaccacg ttccagcctt tgcccagcaa
    18241 atctttggtg atggtgtcaa aactcgaacc gattctgtaa ttcaggttag cttccagaat
    18301 catcaagccg gaatgcagca tacagaacca ggtaaagatc agcgccgcca ttgaccagaa
    18361 aaaccacgcc ccggacatga ccactggcag agaaaacatc cctgcgccaa taatggtgcc
    18421 gccgataatc accacgccgc caagcagcga cggtgacgtt tgggtggtgg ttagtgttgc
    18481 catgagggct tctctccagt gaaaaatagt gcgactgcgt tgttatgtat tgcactgtac
    18541 cagtacacga gtacaaaaga cagaaaaaaa gccccgatgg ttcaaatcgg ggctgtatat
    18601 attattttac agactgtgtt cgctgttcag cgatgattac gcatcaccac caaaacgacg
    18661 acgaccggta gagtcatcac gacgcggagc gcggccttca cgacgttcgc cgctaaaacg
    18721 acgaccatca ccacgaccac cttcacggcg ttcaccgctg aagttacggc cgccttcacg
    18781 acgctcgcca ctgaaaccac gaccaccgcc acgacgctca ccgccagtat gcggctgtgc
    18841 atcgcccagt aattgcatat tcatcggctt gttcagaatg cgagtacgtg taaagtgttg
    18901 cagcacttca cccggcatac ctttcggcag ttcgatggtg gagtgagaag caaacagctt
    18961 aatgttaccg atgtaacggc tgctgatgtc gccttcgtta gcaatcgcac caacgatatg
    19021 acgaacttca acaccatcat cgcggcccac ttcaatacga tacaactgca tgtcaccaac
    19081 atcacgacgt tcacgacgcg gacgatcttc acggtcgcca cgcgggccac ggtcgttacg
    19141 atcgcgcgga ccacggtcat cacggtcacg gaattcacgt ttcggacgca tcggcgcatc
    19201 tggcggaaca atcagagtac gttcaccctg tgccattttc agcagtgcgg cggccagagt
    19261 ttcgagatcc agctcttcac cttctgcagt cggctgaatt ttgctcagca gtgcgcggta
    19321 ttgatccaga tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt tttccagacg
    19381 gcgtttgccc agcagttctg cgttcggcag ttctacttcc ggaatagtca gcttcatagt
    19441 acgttcaatg ttgcgcagca gacgacgctc gcggttctca acgaacagca gcgcgcggcc
    19501 agcacgaccc gcacgaccgg tacgaccgat acggtgaacg taagactcag aatccatcgg
    19561 gatatcgtag ttaaccacca ggctgatacg ctcaacgtcc aggccacggg ctgcaacgtc
    19621 ggtcgcaatc aggatgtcca gacgaccatc tttcaggcgt tccagtgtct gctcacgcag
    19681 cgcctggttc atgtcaccgt tcagcgcggc gctgttgtag ccgttacgct caagagcttc
    19741 agccacttcc agagtcgcat ttttggtacg aacgaagata atcgccgcat caaaatcttc
    19801 cgcttccagg aaacgtacca gtgcttcgtt tttacgcata ccccagacag tccagtagct
    19861 ctggctgatg tcaggacggg tagtcacgct ggactgaatg cgcacttcct gcggctcttt
    19921 cataaagcgg cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga acagagcggt
    19981 ctgatgacct tccgggatct gcgccataat ggtttcaacg tcttcgatga agcccatgcg
    20041 cagcatttcg tcagcttcat ccagaaccag accgctcagt ttagagaggt ccagagtgcc
    20101 acgtttcagg tggtccagca gacggcccgg agtaccgaca acgatctgcg gcccctgacg
    20161 cagggcgcgt aattgcacgt cataacgctg gccgccgtac agggcaacca catttacgcc
    20221 gcgcatgtgt ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca gttcgcgggt
    20281 cggtgccagc accagaatct gtggtgcttt cagctcagga tcaagattct gcagcagagg
    20341 taaagagaat gctgcagttt ttccgctccc cgtctgggcc atacccagaa cgtcgcggcc
    20401 attcagcaga tgtggaatac actccgcctg aattggagat ggtttttcgt aacccagatc
    20461 gttaagggct tcaaggatag gagccttcag gcccagatct gcaaaagtgg tttcgaattc
    20521 agccatgtag tacgtgtgcc tcaaaattaa tggcggccag tctacataac tcatcatgaa
    20581 attgatcagc aattttcatt gaaaagtgtg aaccggctca aagtaggtgt attaacgaac
    20641 aacaacgccc tcacccgtta aggtgatggc aatcaaaaaa gattacgggc tgatgtgtac
    20701 gtcagctatt gctggtccga ttctgccagg tcatcttggt cctggcccag gagcgataat
    20761 tccaacaatg cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac cgccagtttg
    20821 aacagtgccg tggcgctgtc caaatccccc agacttaggt agtacttacc taaatagaag
    20881 ttggtttcac tgagatgctc agcgagcgag gtgttatccg ttgcgtccgc cttgagcctt
    20941 tccattaacg tttgttcgct aatgttgccc aggtagaact cgacaatgtt ccatccccac
    21001 tgttccttat ccgatttttc gaagtgctgt ttcaacactt ctttagcctg cttctcatcg
    21061 agcttctgct cggcgagata aagccacaga ctacggaaag gatcattggg atcgtcttga
    21121 taaaacgcca gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata taatgcgatc
    21181 ccgcgattca agtgcgcgta gttgtaagtt ggatcaagct caagtacaga atcaaacgct
    21241 tcataggcag catcaaaatt gcctgcctgc gttaaatata tgcctaagta attgaatact
    21301 tcaggcatat ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc taatgccctc
    21361 agaccgagac tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg ttcgtcatcg
    21421 gttaaagccc gactggcaag gatttgttcc atacgtgcca gaatcacttc ctgctgtaaa
    21481 gtcggttgca atggtaccgc gaggacttca cttttacgcc aggaagtatt actgcatcct
    21541 gcaagcgtaa gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat ttcccactcc
    21601 cgaagaccac ggttgaatga acgtcctgtt cccggttgct aacaaggcgt cctgcccggt
    21661 taaaagcccc ccgccgcagc ggagggcaaa tggcaacctt actcgccctg ttcagcagcc
    21721 ggagcttccg gtgctgcagc aggttgagac tgctcagtcg cttctttaat gctcagacgg
    21781 atacggccct ggcgatcaac ttccagaact ttcaccggta cttcctgacc catctgcagg
    21841 taatcggtca ctttctcaac gcgtttgtca gcgatttgag agatgtgtac cagaccttct
    21901 ttaccgccgc cgatggcaac aaatgcgccg aagtcaacga tacgggtcac tttaccagtg
    21961 tagacgcggc ccacttcgat ttctgcagtg atctcttcga tacgacgaat agcgtgtttc
    22021 gctttctcgc cgtcggtcgc tgcgatcttc acagtaccgt catcttcgat ttcgatggtg
    22081 gtgccggttt cttcggtcag ggcacggata acagagccgc ctttaccgat aacatctttg
    22141 atcttgtccg ggttgatctt gatggtatgg atacgcggtg cgaactcaga gatatcgcca
    22201 cgcggcgcgt tgatcgcctg ttccattaca cccaggatgt gcagacgcgc acctttagcc
    22261 tggttcagtg caacctgcat gatctctttg gtgatacctt caattttgat atccatctgc
    22321 agtgcagaga taccgtcgcg ggaacccgca actttgaagt ccatatcgcc caggtgatct
    22381 tcgtcgccca aaatgtcaga cagtacaacg tagttgtcgc cttctttcac cagacccatt
    22441 gcgatacccg caacggcagc tttgattggc acacctgcgt ccatcagcgc cagagacgca
    22501 ccgcacacgg aagccataga agaagaaccg ttggattcag tgatttcaga cacaacacgt
    22561 acggtgtacg ggaatttgtc catatccggc attactgcca gcacgccgcg cttcgccaga
    22621 cgaccgtgac caatttcacg acgcttcgga gaaccgacca tgccggtttc gcctacggag
    22681 tacggaggga agttgtagtg gaacaggaag gtatcggtac gttcgcccat cagttcatca
    22741 agaacctgcg cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg cgtttcacca
    22801 cgggtgaaca gcgcagaacc gtgagtacgc ggcagcacgc cagtacgcac atccagacca
    22861 cggatcatat ctttttcacg accatcgata cgcggttcgc ctgccagtac gcggctacga
    22921 acaacgtttt tctcgatcgc gtgcagaatt tcacccagtt cgttttcgtc cagggtttcg
    22981 tcttcagcaa gcagcgtcgc gatggtttca gatttgatga catcaacctg cgcataacgc
    23041 tcttgtttgt cggtgatgcg gtaagcatcg ctcagacgag cttcagccag tgcagcaacg
    23101 cgcgcgttca gcgcttcgtt taccggctcc ggctgccagt cccaacgcgg tttaccggct
    23161 tctttcacca gttcattgat gttctgaata acaacctgct gttgttcatg accgaacact
    23221 actgcgccca gcatctggtc ttcgctcagc agttcagctt cagattcaac catcagtaca
    23281 gcggcttcag tacccgcaac aaccagatcc agtttgctct ctttcagctc gtcctgagtc
    23341 gggttcagta cgtactggtc attgatgtaa cctacgcggg cagcaccaat cgggccattg
    23401 aacggaatac cagacagaga cagtgctgcg gaagcaccaa tcatcgcgac gatatccggg
    23461 ttaacctgcg ggttaacaga aaccacggtg gcgataacct gaacttcgtt gacgaagcct
    23521 tccgggaaca gcgggcgaat cgggcgatca atcagacgcg cgatcagggt ttcgccttcg
    23581 cttggacgac cttcacgacg gaagaagcta cccgggatac gaccagcagc gtaggtacgc
    23641 tcctgatagt taacggtcag tgggaagaag tcctgacctg gtttggcttt tttctggcca
    23701 acaacggtaa caaataccgc ggtgtcatcc atgctaacca taacagcggc agtagcctga
    23761 cgagccatca tgccggtttc cagagtcacg gtgtgttggc cgtactggaa tttacgaacg
    23821 atcggattaa gcaatgtaat atcctttctc tttcttagac agtaccttac ggcactggtg
    23881 ttaatacccg atcttctgcg catcctcgcg actaatgaca accctaaccc agctctatgt
    23941 gggtaaagcc tctcattagc cgcgcgaacc tctgcaacgg aagatcattc atagcaacaa
    24001 tacattagtt tccagtgaat tgctgccgtc agcttgaaaa aaggggccac tcaggccccc
    24061 ttttctgaaa ctcgcaagaa ttagcgacgc agacccaggc gctcgatgag ctgggtgtaa
    24121 cgtgctacgt ctttacgttt caggtagtcg agcagtttac gacgctgaga aaccatgcgc
    24181 agcagaccac gacggctgtg gtgatctttt ttgtgctctg caaagtggcc ctgcaggtgg
    24241 ttgatctgtg cagtcagcag tgctacctga acttcggtag aaccggtgtc gtttgcgtca
    24301 cgaccaaact cagaaacgat tttagctgtt gcttcagtac ttagagacat tttaaaactc
    24361 caaagtatat agaatgaaag gacgccgatc tctaattcag cgatcccagt gtacgttacg
    24421 caaagtgtta aacaatttac gcgacgttaa gcggcagtat tctactcgta gcgacctgtt
    24481 atcgcaagac ggttaacatt acgccgggta ttcaaccacc agacgacgag gcgcaacgcg
    24541 gccttcatcg tcaatttcgc ccataccgat aaatttgccg ttctcacctt ccgtgacgcg
    24601 aaccagtcct tccagcggcg cgccagatgt acgaaccggg ttaccatttt tgaagtaaac
    24661 agaagacgtt aacggaagat tcaccaccgg atagtccgaa gctggactgt ccattggcat
    24721 cagtaatgga tcaagtaact ccgcagctgg aatatcctgc tgttcggctt gttcgaccag
    24781 ttcacgcaga tgttccaggg tcaccatccg ttcaaccgga tatttactta ccgccagacg
    24841 gcgcaggtaa ataacatgcg cgccacaacc gagtttctca cccaggtcgt caatgatagt
    24901 gcggatataa gtgccttttg agcagtgaat ttccagctcc agctcattgc cttcatggcg
    24961 aataaacagc aattcataaa cggtaatcgg acgcgcttca cgcggaactt caatgccctg
    25021 acgcgcatat tcgtacagtt ttttgccctg gtatttcagt gctgaataca tcgatgggat
    25081 ctgttcgata tcgccacgga aagtatccag tgccgccgcc agttgttctg cactaaaggt
    25141 taccggacgc tcttcaacga tttgtccgtc ggcgtcagaa gtatcagtac gctgtccaag
    25201 acgcgcaatg acccgatagc gtttgtcgga gtccagcaga tactgggaaa acttcgtcgc
    25261 ttccccgagg caaatcggca acatgccggt cgccagcggg tccagcgcac cggtatgacc
    25321 ggcacggttg gcgttataaa tccgtttcac tttttgcagc gcatcgttgc tggacatacc
    25381 ctgaggttta tccagcaaca aaacgccgtt aatgtcgcga ccgcgacgac gaggacgact
    25441 cattagtcct ccttgctgtc gtccgggtta acacgacgtt cttcgtcatg tttgaccacg
    25501 ctggtcacca ggtttgacat gcgcatccct tcaaccagag agttgtcgta gaagaaggtc
    25561 agttccggca cgatacgcag gcgcatcgct ttccccagca ggctgcggat gaaaccagaa
    25621 gcttcctgca acgctttgat gcccgcttta actgcgtctt catctttgtc gttgaggaaa
    25681 gtgacataca ctttggcata cgccaggtcg cgagacattt cgacaccgga aacggtggtc
    25741 atcatgccca gacgaggatc tttaatttca cgctgcagga tgagagcgat ctctttttgc
    25801 atttcctgcg ctacgcgctg cgggcgacca aattctttcg ccataataaa ttctcctgac
    25861 aaaaaagggg ctgttagccc ctttttaaaa attaatttca ggtggaaggg ctgttcacgt
    25921 tggcctgata agacgcgcta gcgtcgcaac aggcaatcca tgccggatgc ggcgtaaacg
    25981 ccttatcccg catagaaccc taaaaacctt aagcgatggt acgttggatc tcgatgattt
    26041 cgaatacttc gatcacatcg ccagtgcgga cgtcgttgta gttcttaacg ccgataccac
    26101 attccatacc gttacggact tcgttaacgt catctttgaa gcggcgcagg gactccagct
    26161 cgccttcata gataaccacg ttgtcacgca gaacgcggat cgggttgtga cgtttaacca
    26221 caccttcggt aaccatacag cctgcgatgg caccaaattt cggcgatttg aacacgtcac
    26281 gaacttccgc cagaccgata atctgctgtt tcagttccgg agacagcata ccgctcatcg
    26341 ccgctttcac ttcgtcgatc aggttataga tgacggagta gtaacgtaga tccaggcttt
    26401 cggattcaat cactttacgt gcagaggcat cagcacgtac gttgaagcca accaggatgg
    26461 cgttggatgc cgcagccagg gtggcgtcgg tttcggtgat accacctacg ccagaaccga
    26521 tgatcttcac tttaacttcg tcagtagaca gtttcagcaa ggagtcggag atcgcttcca
    26581 cagaaccctg tacgtctgcc ttcaggacga tattcacttc gtgaacttcg ccttcggtca
    26641 tgttggcgaa catgttctcg agtttagatt tctgctgacg cgccagttta acttcgcgga
    26701 atttaccctg acgatagagt gcaacttcac gcgctttctt ctcgtcacgt acaacggtaa
    26761 cttcatcacc cgcagccgga acaccggaca agccgaggat ttccaccgga atggacggac
    26821 ccgcttccag cacttcctga cccagttcgt tacgcatcgc acgaacacga ccgtattcga
    26881 agccacacag aacgatatcg cccttgtgca gagtaccttc acgtaccaga acggtagcaa
    26941 ccggaccacg acctttatcg aggaaggatt cgataaccgc accgctcgcc atacctttac
    27001 gtaccgcttt cagctccaga acttccgcct gcagcaggat agcgtccagc agttcatcga
    27061 taccggtacc cgctttcgca gatacgtgta cgaactggct ttcaccgccc cactcttccg
    27121 gcaggatgcc gtactgggag agttcgttct taacgcgatc cggatcggct tctggtttat
    27181 cgatcttgtt cactgcaacc accaccggta cctgcgccgc tttcgcgtgc tggattgctt
    27241 cgatggtctg cggcattacg ccgtcgtcgg cagcaacaac caggactacg atgtcagttg
    27301 cctgcgcacc acgtgcacgc attgaagtaa acgcggcgtg ccccggggtg tccaggaagg
    27361 tgatcatgcc gttttcagtt tcaacgtggt atgcaccaat gtgctgggta atgccgcccg
    27421 cttcgccaga ggccactttc gttgaacgaa tgtagtccag cagagaggtt ttaccgtggt
    27481 caacgtgacc catgatggtc acaaccggag cgcgcggttc agccgcagca cccgtgtcac
    27541 ggtcgctcat taccgcctct tccagctcgt tttcacgacg caggataact ttgtggccca
    27601 tctcttcagc aaccagctgt gcggtttcct gatcgataac ctggttgatg gttgccattg
    27661 cgcccagttt catcatcgct ttgatgacct gagagccttt aaccgccatc ttgttcgcca
    27721 gttcgccaac ggtgatagtt tcgccgatca caacgtcacg gttaacggcc tgagcaggct
    27781 tctggaagcc ttgctgcagc gaagaacctt tacgttttcc gcccttaccg ccacgtactg
    27841 ctgcgcgtgc ttcttcacga tcagcttttg attcagcgtg tttgttgcct ttcttcggac
    27901 gcgctgcttt cgcgttacga ccacggccac ggccgccttc gacttcacga tcgctttcgt
    27961 cttctgcctg gcgagcatgt tgagaagtag tgacgtgata atcgctggaa tcctcagtcg
    28021 gttccgcgtt atcagtccat ttgttttctt ccgccatacg acgtgcttct tcagcaacgc
    28081 gacgtgcttc ttcttcgagt ttacgacgcg cttcttcttc ggctttacgc ttgagctctg
    28141 cagcttcctg ctcacggcgg gctttttcag cctgggcgtt tttagtcata tcgtcttgtt
    28201 gattgctcac tttgtctttt tccgcagctt cacgtttcgc ttgttcagca gcttcacgct
    28261 tagcttgttc tgcggcctca cgttcagctt tttgttgcgc ctcgcgttta gccgattctt
    28321 ctgcctcacg acgggcttgc tcttccgctt cacgctgcgc ttgctcttcc gctgcaaggc
    28381 gttcagcctc ttgcggatcg cgtttcacaa aggtgcgttt cttgcggact tcgatttgta
    28441 ccgatttgct ttttccaccg gtaccaggaa tgttaagggt gctgcgtgtt ttacgttgca
    28501 gcgtcaattt gtccgggcct gaatttttct gattcaggtg gtcaatcaaa gtctgtttct
    28561 cttgtgcaga cacagagtcg tcagcagact tccggatacc tgcatcagca aattgctgta
    28621 ccaggcgttc cacggaggtc tgtcgctctg cggccagcgt tttaatcgtt acatctgtca
    28681 tgctgttcct tcctgctaca gtttattacg cttcgtcacc gaaccagcaa atattacggg
    28741 cagccataat cagtgctccg gctttttcgt cggtcaaccc ttcgatatca gccagatcat
    28801 caatgccctg ttcggcgaga tcttccagcg tacaaacgcc acgggcggcc agtttgaatg
    28861 ccaaatcacg atctacccct tcaaggttca gcagatcgtc agccggtttg ttatcaccga
    28921 ggctttcttc ctgggcctgt gcaatggtgg ccagtgcatt tttagcacgc tcgcgcagtg
    28981 cttcaacggt cggctcatca aggccttcga tttccaacag ctctttcatc ggcacatagg
    29041 ccagttcttc cagcgtcgag aagccttcct ctaccagaac agtcgcgaag tcttcgtcga
    29101 tgtcgagata tttggtgaag gtgtcgatcg ctgcgtgcgc ttccgcctga tgcttagcct
    29161 gcaggtcgtc aacggtcatc acgttgagtt cccaaccgct cagctgcgaa gccagacgca
    29221 cgttctgacc gttacggcca atcgcctgcg ccaggttacc ggcttcaacg gcgatatcca
    29281 tggtgtgttt atcttcatcc accacgatag aagcaacgtc tgctggtgcc attgcgttaa
    29341 tcacaaactg cgccgggtta tcatcccaca ggacgatatc gatacgctcg ccgcccagtt
    29401 cagtagaaac cgcctgaaca cgcgcgccac gcatacctac gcaagcacct accggatcga
    29461 tacgtttatc gttggttttc accgcgattt tcgcacgaga acccgggtcg cgagccgctg
    29521 ctttaatttc aatcacttct tcgccgattt ctggcacttc aatacggaac agttcgatca
    29581 gcatttccgg cttggaacga gtgacgaaca gttgcgcgcc acgcgcttcc gggcgaacgg
    29641 aataaagcac gccacgaacg cggtcgccag ggcggaagtt ttcacgcggc agcatatctt
    29701 cgcgcaggat cacggcttca gcgttgttgc ccagatccag agagatgttg tcgcggttta
    29761 cttttttcac cacgccggtg atgatttcac cttcgtgttc acggaactga tcaaccacca
    29821 tcgcacgttc ggcttcacgc actttctgca cgataacctg ttttgccgtc tgggtagtga
    29881 tacggtcaaa ggtaacagac tcaatctgat cttcaacgta atcgcccagg ttcaggcttt
    29941 catcttcata acgtgcggct tcaagggtga tttccttggt cggctgggtg acttcatcaa
    30001 caactaacca gcgacggaag gtgtcaaaat caccgctttt gcgatcgatc tgtacgcgga
    30061 cgtcgatctc ttgttcatat tttttctttg ttgctgtcgc cagcgcgctt tccaatgctt
    30121 cgaaaatctt ctcgcgaggt agcgcctttt cattggatac ggcttcaact acagccaaaa
    30181 tttctttgtt catcgcgggc ttttcacctc atccagacta ttaaaagtgg ggaaccaggt
    30241 tcgccttctg gatattactc agcgcgaaca cttcatcttt accttcgacg gtaactgtga
    30301 tcatttcacc gtctaccgct ttgataacgc cctgccattt acgacggttt tgtaccgcca
    30361 tacggagaac cagagtcacc tcttctccga caaaacgggc gtagtgttca gccgtgaaca
    30421 gtgggcgatc gagacccggt gaggagactt ccaggttata agcaacggtg atgggatctt
    30481 caacatccag cacagcactt acctggtggc tcacatcagc acaatcatca acattgatgc
    30541 catcttcact atcaatatag atgcgcagtg tggatgtgcg accgcgaata aattcgatgc
    30601 caaccagttc aaaacccagg gcctcaactg gcgcagtaat catctctgtt aatttttgct
    30661 ctaatgtgga caagcccacc cccaagacat aaaaaaaggg cctaaagccc agttattctg
    30721 tagtcagata acaaaaaacc ccgataaatc ggggctttat ataactgaac cctataaccg
    30781 caactgcggt ctggagcact ttccagaagg attttttcaa atcccactac gaaggccgaa
    30841 gtcttcacag tatatttgaa aaaggactct aagggaaagt ggttgcgggg gccggatttg
    30901 aaccgacgac cttcgggtta tgagcccgac gagctaccag gctgctccac cccgcgcctg
    30961 aaacgtggca aattctactc gttttgggta aaaaatgcaa atactgctgg gatttggtgt
    31021 accgagacgg gacgtaaaat ctgcaggcat tatagtgatc cacgccacat tttgtcaacg
    31081 tttattgcta atcacgtgaa tgaatatcca gttcactttc atttgttgaa tacttttgcc
    31141 ttctcctgat cccccttaag cgcattattt tacaaaaaac acactaaact cttcctgtct
    31201 ctgataaaag atgattaaat gaaaactcat ttattttgca taaaaattca atgagagcgg
    31261 aaatccaggc tcatcatcag ttaattaagc agggtgttat tttatgacga cgattctcaa
    31321 gcatctcccg gtaggtcaac gtattggtat cgctttttcc ggcggtctgg acaccagtgc
    31381 cgcactgctg tggatgcgac aaaagggagc ggttccttat gcatatactg caaacctggg
    31441 ccagccagac gaagaggatt atgatgcgat ccctcgtcgt gccatggaat acggcgcgga
    31501 gaacgcacgt ctgatcgact gccgcaaaca actggtggcc gaaggtattg ccgctattca
    31561 gtgtggcgca tttcataaca ccaccggtgg cctgacctat ttcaacacga cgccgctggg
    31621 ccgcgccgtg actggcacca tgctggttgc tgctatgaaa gaagatggcg tgaatatctg
    31681 gggtgacggc agcacctata aaggaaacga tatcgaacgt ttctatcgtt acggtctgct
    31741 gaccaatgct gaactgcaga tttacaaacc gtggcttgat actgacttta ttgatgaact
    31801 gggcggtcgt catgaaatgt ctgaatttat gattgcctgc ggtttcgact acaaaatgtc
    31861 tgtcgaaaaa gcttactcca cggactccaa catgcttggt gcaacgcatg aagcgaagga
    31921 tctggaatac ctcaactcca gcgtcaaaat cgtcaacccg attatgggcg tgaagttctg
    31981 ggatgagagc gtgaagatcc cggcagaaga agtcacagta cgctttgagc aaggtcatcc
    32041 ggtggcgctg aacggtaaaa cctttagcga cgacgtagaa atgatgctgg aagctaaccg
    32101 catcggcggt cgtcacggtc tgggcatgag cgaccagatt gaaaaccgta tcatcgaagc
    32161 gaaaagccgt ggtatttacg aggctccggg gatggcgctg ctgcacattg cgtatgaacg
    32221 tctgcttacc ggtattcaca acgaagacac cattgaacag tatcatgcac atggtcgtca
    32281 gttgggccgt ctgctgtacc agggacgttg gtttgattcc caggcgctga tgctgcgtga
    32341 ctctctgcaa cgctgggttg ccagccagat cactggtgaa gttaccctgg agctgcgccg
    32401 tgggaacgat tattcaatcc tgaataccgt ctcagagaac ctgacctaca agccagagcg
    32461 tctgacgatg gaaaaaggcg actcggtgtt ctcgccagat gatcgtattg gtcaattgac
    32521 catgcgtaac ctggatatca ccgatacccg cgagaaactt ttcggttatg ccaaaaccgg
    32581 cctgctttcc tcctctgccg cttcaggcgt gccgcaggtg gataatctgg aaaacaaagg
    32641 ccagtaattc gacatcaacc ctgcccttca ggggtggggt taccttgctt atcctctgta
    32701 aactcctttc tgagcatcac ataatcgaaa acatcatggt ttgcatccac aacccgtgag
    32761 tctcctttat agtgtgcaat cacttcctca agcgtttgcg gctgttccgg ctttgttacc
    32821 cccatccacg cattaataag gtaattattg taagctgtcg aaaagatgtt gttttccgtt
    32881 gtgcgatcca caccatcccc aagaacgggg ctataccaga taaacatcgg aacatgatat
    32941 gcctgctggc tggcctccct gcctccatga aaatagacgt tcttcttcgt agggtcacgt
    33001 tccaggccgt ggtcggcaaa atacatgacc gaggcgcggc gatcttttaa taattcaaaa
    33061 acctgaccta gcaaactatc tgtgtaatga atggagttgt catagcaggc atcctgatcg
    33121 tcctgcggtt gaaacacggc gctggattgc ggataggcgc tacaagccgg ttcatggctt
    33181 ccatttaaat gaagaacaat cagtttcttt tgctgcgtat tttgctgtaa tgcttgcgat
    33241 aaatgcggca acaacaattc atcaaatcct ctgacataaa ctgtttccat ggcacgcatg
    33301 gcgatactgg taactgctgt accattctgc cgaaaagcgg attgcgagct tagccagaaa
    33361 gtctgaaatc ctgcttgatt agccatatta ataatgttgt cggggtaatt atgaatgtca
    33421 tggctcaaaa cagaatcagc agttaaagaa aggggaaccg acagcgcagt gtaaggtgca
    33481 ccgcttattg cttgattaaa cagtttgatc tgttttcttt gtgcttcgac ttgcggtgtc
    33541 gtagagcgtg tatatccata caaagacata ttgtcgacac gtacagactc ccccacaatc
    33601 aacacgtagg tatcaatacc tgtatccctg actgataatt gaaaatacgg cactgtgttt
    33661 gcaattgaga gtaatctttg atgctctttc gctgctaaag caaaataatt gagattgaaa
    33721 aacggcgtat aggtagcaaa tcgcgacgct agtatatatg gactgaacgc atttttattt
    33781 ttggcatctt tataagcaaa ttgacaagcg gaaaataaac ttcccgaaat gacaatcagc
    33841 aataatattc ctgtcacttt ttttgtcggc aaggaaacat catattttat tattacagcc
    33901 aaaaaaagaa gggataaaaa ggcaaaagca catagataag ggctatacat tcccagcatt
    33961 ttggcaactt catccggatc actctgcagc acactaatcg caaatccatc attgaaagtt
    34021 gtaccaaagg tacaccatga atagaggcta atactcatgt ctgccgcagt cagtaacaca
    34081 aatggtgcgg caacaagcag gcgaagcgga agggatgata tacgtttaac aaccaggatg
    34141 gtcagaacga agaacagcaa tcgctgaacc atatgcgctc cggaagaggc gactaagttc
    34201 gtaataccaa aaacaataac actaagatac aacaaccaat gagatttaaa acttctagca
    34261 aatttgttga atactgtcat ccagaaatcc aagttcacaa ccaatataaa caattatcaa
    34321 caatgtgcaa ttttatatca gacatgtttt tcaactctga tccatatcag aacgaaacac
    34381 tgttagtttt ttctttttaa atgatgcggc cattgcgttc tcagtaatta aataacatta
    34441 tcaatgcgtc tctaaacatt acgcagaacg tagccatcag tagttatact gacaggagca
    34501 ggatggttta tcgagaagta aacatatccg tattcgtacc ggaactggag ttaacacaat
    34561 aaagtgccaa ttatgtcagc agtagggaaa agtttataac gaaggcgtac cggcaccatc
    34621 gtttcaaggt gccagctacg agtaaagcaa ctggacgaga tacagatacc tgacaaccat
    34681 tcctcagacc aggaccaaaa cgaaaaaaga cgcttttcag cgtctctttt ctggaatttg
    34741 gtaccgagga cgggacttga acccgtaagc cctattgggc actaccacct caaggtagcg
    34801 tgtctaccaa ttccaccacc tcggcacgga aactactttt tagttcggga tatcgctggt
    34861 cggcttagcc ggagcagctg gctgagtttg ttcggttttc gccggcgcac tcaggttttc
    34921 ccattcgcta cctttattgg ttttgttgct attgatgtta cccagcacca gactgatgat
    34981 gaagaataac gttgccagca acgccgtcat gcgggtcatg aagttaccag aaccacttga
    35041 accaaacagc gtagcggaag cgcctgctcc gaaggaggct cccatatcag cgcctttacc
    35101 ttgctgcagc atgatcagac caacaaggcc aattgccaca ataaggaaaa ctaccaaaag
    35161 agcttcgtac ataatcaacc tgttccttgc ggagttgccg cgtaccaatg cttcaaccaa
    35221 taaagcggga gtttttaatc tttcccactg aagcgggtgt gaatactaac caaagcgaat
    35281 gaccttcgca agggcaattt tatcgcattg tatcaactgc ggaaaaaaac agcaaaaccg
    35341 atgtgttcgc cgagaaaaaa ggcgacaatc gccacctttt tagccagtta tctaacgctt
    35401 taaacggctt ttactgcatc ggcgatgcgg tgtgcaaatt cagtcacctg cgcttcgtct
    35461 tcgccttcca ccatcacgcg aattaacggt tcggtgccgg atttacgcag caacacgcgt
    35521 ccacggttgc ccagcgccgc ttcaacctct gcggtcacag ctttgactga ttcatgctcc
    35581 agcggatcgc cgctacctgc ggtgtaacgc acgtttacca ggatttgcgg gaacattttc
    35641 atgccgctgc aaaggtcgtg caggctcata tggttacgcg ccatcgccgc cagcacctgc
    35701 aaaccagcaa cgatgccgtc accggtagtg gttttatcca gcaggatcac atgaccggaa
    35761 ttctctgcac cgatacgcca gcctttctcc tgcatttttt ccagtacgta gcggtcaccc
    35821 actttcgcgc gcgcaaatgg aattcctaac tgtttcaacg ccagttcaag ccccatgttg
    35881 ctcatcaatg tacccacagc gccaccacgc agctggccct gacgaagacc ttcacgcgcg
    35941 atgatataca tgatctgatc gccatcgact ttattgcctt catggtcaac cataatcacg
    36001 cgatcgccgt cgccgtcgaa ggcaataccg agatccgctt tttcagccag cacacgagcc
    36061 tggagcgcgc gaacgtcggt agcccccact tcggcattga tgtttacacc gttcggctca
    36121 caaccgatag cgataacgtt cgcccccagt tcgcgcagca cgttcggcgc gatgtgataa
    36181 gtcgcaccgt ttgcacaatc caccacaatc ttcagttcac taaggctaag ttcgttcggg
    36241 aacgtggctt tgcaaaactc gatatagcga cccgcagcat caacgatacg gctggcttta
    36301 cccagttctg ccgaatcaac gcagctgatc tccttttcca tttccgcttc gatggcctct
    36361 tctaccgcat ccggcagttt ggtgccgtcg atagagaaga atttaatgcc attatcgtag
    36421 aacgggttat gcgatgcaga tatcacaatt ccggcctctg cgcggaaggt acgcgtcaga
    36481 taagccacgg ccggtgttgg catcgggcca gtgaagagtg cggaaaggcc cgctgccgcc
    36541 agacccgctt ccagtgctga ctccagcata tagccagaaa tacgcgtgtc tttaccaata
    36601 ataatcttac gggagccgtg gcgcgccagc actttacccg cggcccaacc cagcttaagc
    36661 acaaaatcag gggtgatcgg cgcatcccct acacgaccac gaatcccatc ggtaccgaaa
    36721 tatttacgat tactcatagc gtttgttttc ctttgcagac agagtggctt ccaccacccg
    36781 catcgcttct acggtttctt tgacgtcatg aacacgaatg atgtgcgcgc cttgcattgc
    36841 ggcaatgacc gcacaggcca gactaccgct caggcgctcg gacggcccca cgttcagcag
    36901 ctgcccaatc atcgattttc gtgacatacc caccaacagc ggcaggttga aatggtgaaa
    36961 ttcagccagg cgcgccagta atgaatagtt atgggagaga tttttaccga aaccgaatcc
    37021 ggggtcgagc aacaatttct cttttgcgat acccgcctgc tcgcaacgtg ctatttgctc
    37081 aataaagtag cgattcactt ctgcaaagac atcgtcatac ttcggagctt cctgcatggt
    37141 tttgggattt ccctgcatat gcatcagaca aaccggtaaa ccggtttctg cagccgcctc
    37201 cagagcgcca ggttcggaaa gggagcggat atcattaata atgtgagcgc caacttttgc
    37261 tgactcacgg atgacttctg gtttggatgt atcgaccgag atccagactt cgaagcgttg
    37321 agcaattgcc tcaaccacag gaataacacg ttgcaactct tcttcaacgc taacttccgc
    37381 cgcccctggg cgcgtggact cgccaccaac gtcaatgatc gtcgcgccag cgttgatcat
    37441 cagattcgca tgtttcaccg catctatcag cgagttatgc gtgccaccat ccgaaaagga
    37501 atcaggcgtg acgttgagga tccccattac gtgaggatgg ctaaggtcca gtgaagtacc
    37561 ctgggcaaag agtttcatga tgttatccct ggtatgaatt gataagaaaa aaccccggag
    37621 cacgccccgg ggttttcggt acaaatacag tcatctgatg cgggaactta cttgtcgcct
    37681 aactgctctg acatggtgtt acccgggttc ggcgtacgcg gttcatcaac cggacgagga
    37741 gcctttggac taccattgtc gccagaattg ttagaagcgc ctggttcttc ccagcccgct
    37801 ggcggacgta catcgcgacg tgccatcagg tcatcaatct gcggtgcgtc gatagtctca
    37861 tatttcatga gagcatcttt catcgcatgc agaatatcca tattgtcggt cagaagctga
    37921 cgcgcacgat tatagttacg ttcaatcagt gctttcactt cctggtcgat gatacgtgca
    37981 gtttcatcgg acatatgttt cgctttcgct acgctacggc cgaggaacac ttcaccttct
    38041 tcttccgcgt acagcagtgg acccaatttc tcagagaagc cccactgagt caccatgtta
    38101 cgtgccaggt tggtcgcaac tttaatatcg ttggacgcac cggtagaaac atgttccggc
    38161 ccgtagatga tctcttctgc cagacggccg ccgtacagcg tagaaatctg gctttccagt
    38221 ttctgacggc tggcgctgat tgcgtcgccc tcaggcagga agaaagtcac acccaacgca
    38281 cgaccgcgtg ggataatcgt cactttgtgc accggatcgt gctccggcac caggcgaccg
    38341 ataatcgcat gacccgcttc gtggtaagcc gtcgattctt tctgcgcttc cgtcatcacc
    38401 atggagcgac gttccgcacc catcatgatt ttgtctttcg ctttctcgaa ctcaaccatc
    38461 gacacaacgc gtttgttgcc acgagcagcg aacagtgccg cttcgttcac caggttcgcc
    38521 aggtcagcac cggagaaacc aggagtacca cgggcaatga ttgccgcgtc gatatcgggt
    38581 gccaatggta cgcgacgcat gtgaaccttc aggatctgct cacgaccgcg aacatctggc
    38641 aagccaacca caacctgacg gtcgaaacgg ccaggacgca gcagcgctgg gtcgagaacg
    38701 tccggacggt tagtcgcggc gataacgatg ataccttcgt taccttcgaa gccatccatc
    38761 tcaaccagca tctggttcag agtctgttca cgttcatcgt gaccaccgcc cagaccagcg
    38821 ccacgctggc ggcctacggc gtcgatttca tcgataaaga tgatgcacgg tgccgctttc
    38881 ttcgcctgtt cgaacatgtc acgaacacgg gatgcaccca caccgacgaa catttctacg
    38941 aagtcagaac cggagatagt aaagaacgga actttcgctt cgcctgcaat cgctttcgcc
    39001 agcagcgttt taccggtacc cggaggaccg accatcagga cgcctttcgg gatcttaccg
    39061 ccgagtttct ggaagcggct cggctcgcgc agatactcaa ccagttcagc aacttcttct
    39121 tttgcttcgt cgcagcccgc aacgtcagca aaggtcgttt tgatctgatc ttccgtcagc
    39181 atgcgcgctt tgctcttacc aaacgacatg gcacctttgc caccgccgcc ctgcatttga
    39241 cgcatgaaga agatccagac accaatcagc aacagcatcg ggaaccagga gatgaagata
    39301 gaagccagca ggcttggttc ttcaggcggt tcaccgacaa ccttgacgtt cttggtcaac
    39361 aggttatcca gtagtttcgg atcctgaacc ggaatgtaag tggtataacg gttactatct
    39421 ttcttggtaa cgttgatttc acgtccgttg atacgcgctt cacgaacctg gtcgttattg
    39481 acctcttgta ggaaggtaga gtaatccacc ttacggccat tagactcgct gggcccaaag
    39541 ctctggaata ctgacatcag cacaacggca atgaccagcc agagtattag gtttttcgcc
    39601 atgtcactca agggattaac ctcttattac aactgtgtta aaaacagcgt caggatactc
    39661 tatatccagc atctttcaaa ctttcgtctg aaatctcccg gttagggttt acgcccggtc
    39721 gctacaatat acacttcccg cgaacgtgca cgagaagagt ccggcttacg aactttgacc
    39781 ttcgtaaaca gggagcgaat ttcccttaga tactcatcga aaccttcgcc ctggaacacc
    39841 ttcactacaa aactgccacc tggcgctaat acatcacgac acatttctag cgccagttcc
    39901 accagataca tggcacgggg gatatccacc gccggtgttc cgctcatgtt tggtgccata
    39961 tcggacatga caacctggac tttgctgtcg ccaacgcgct ccagcagtgc tttcatcacc
    40021 agttcatcac gaaaatcgcc ctgaagaaag tccacaccaa cgataggatc cataggtaaa
    40081 agatcgcaag cgatgatgcg gcctttgccg ccaatttggg tgaccacata ttgtgaccaa
    40141 ccacccggag cagcaccaag gtcgacaacc gtcattcccg gtttaaagag tttgtcactt
    40201 tgctgtattt catcaagttt aaaccaggca cgggaacgta accccttttt ctgtgcctgt
    40261 tgaacatatt tatcgctaaa gtgttcctga agccagcggc tggagctggc agaacgcttc
    40321 ttacctgtca tttaactttc ccatggggat aactcatcgt aaccaattgc gtaaattttt
    40381 acgcgcctat ttggtgatat atgggagatg gcggtagaat gacccgtttt caatcccaac
    40441 gtaagcaaaa aatacgatga atctgagtac taaacaaaaa cagcacctga aaggtctggc
    40501 acatccgctc aagccagttg ttctgcttgg cagtaatggt ttgaccgaag gggtgctggc
    40561 cgagattgaa caagcgttag agcaccatga actcatcaag gtgaaaatcg ccaccgaaga
    40621 tcgcgaaact aaaaccttga tcgtggaagc tatcgtgcgc gaaaccggcg cctgtaatgt
    40681 acaggtcatc ggtaaaacgc tggtgcttta tcgcccaact aaagaacgta aaatctcgct
    40741 gccacgctaa gattatccta aagttacaca catttgctgt gtaaaacgag gggttttccg
    40801 caggcaggag agcaaaatgc cacgctctgt tcgttgataa aaggccgcat agcggccttt
    40861 ttcctttctt tacaatacat caacatcttg agtattgggt aattcttaca ggtattccac
    40921 cttaattact tcaaattcta cttcgccgcc cggcgttttg atgaccacaa catcatcttc
    40981 ttctttgccg atcaggccac gagcaatagg tgagtttaca gaaatcaggt tttgtttaaa
    41041 gtcagcttcg tcatcgccaa cgatgcgata agtctgttct tcgtcagaat cgagattcag
    41101 caccgttacg gtagcaccaa aaataacgcg cccattgttg ggcattttgg tgacatcaat
    41161 cacctgcgcg ttcgacagct tggcttcgat gtctttaata cggccttcgc agaaaccctg
    41221 ctgttcacga gctgcgtggt attcggcgtt ttctttcagg tcgccatgct cacgcgcttc
    41281 cgcgatagca gcaatgattt caggacggcg cacagatttc agaaaatcca gctcttcgcg
    41341 taatttttca gcgccgcgta aggtcatcgg aatagcttgc atttgttata cctcttgaat
    41401 attcctgatg gggcaagtct tcacccaccc cggctgttcg gcctgcccgg cataactcct
    41461 caccgggacc agaagcaaaa aaataccgac ccgggtacaa gtcccaggtc agctacaatt
    41521 cacattttga tagtcatttt accctgaagt tcccgaaggg tcatcgttta ctttataggg
    41581 cgttgcgccg tagtatgacg gctcgattcc aggttgttag cgcgagatta tgcgattttc
    41641 cagatttatc atcggattga ccagctgtat agcgttcagt gttcaggccg caaatgttga
    41701 tgagtacatt actcaactcc ccgctggtgc caaccttgcc ctgatggtgc aaaaagtcgg
    41761 cgcgtcggcc cccgctattg attaccacag tcagcagatg gcgctgcccg ccagtaccca
    41821 gaaagtgatt actgcgctgg cggcgttgat tcaactcggc cccgattttc gttttaccac
    41881 gacgcttgaa accaaaggca atgtggaaaa cggcgtactt aagggtgact tagtggcgcg
    41941 atttggtgcc gatccgacgt taaaacgtca ggatattcgc aatatggtcg cgactttgaa
    42001 aaaatctggc gtcaaccaaa tcgatggcaa tgtgttgata gatacctcca ttttcgccag
    42061 ccacgataaa gcccccggct ggccatggaa tgacatgaca caatgcttta gcgctccgcc
    42121 tgccgccgcc atagttgacc gcaactgttt ctccgtctcg ctctacagtg ccccaaaacc
    42181 tggtgatatg gcttttatac gcgtggcatc ttattacccc gttacgatgt tcagccaggt
    42241 acgcaccctc ccccgtggtt ctgccgaagc gcaatactgc gaactggatg tcgtaccagg
    42301 cgacctgaac cgctttacgc tgacgggatg cctgccacaa cgttctgagc cgctcccgtt
    42361 ggcttttgcc gtgcaggatg gagccagcta tgccggtgca attctgaaag atgagttaaa
    42421 acaggcgggt atcacctgga gcggaacact gctgcgccag actcaggtta acgaacctgg
    42481 aacggtagtt gccagtaaac agtcggcccc gctgcacgat ctgcttaaga ttatgctgaa
    42541 aaagtcggac aacatgatcg ccgatacggt tttccgcatg ataggccata cgcgcttcaa
    42601 tgtgcctgga acatggcggg ccgggtcgga cgctgtgcgt cagatcctgc gccagcaagc
    42661 cggtgtcgat attggaaaca ccattattgc cgatggttca gggctttcgc ggcataacct
    42721 gattgccccc gccaccatga tgcaggtgct gcaatacatt gcccaacacg acaatgaact
    42781 taactttatc tccatgctgc cgctggcggg ctatgacggc tctttgcagt accgtgcagg
    42841 tctgcatcag gcgggcgtgg atggaaaagt ctcagcgaaa accggttcgt tgcagggggt
    42901 atataacctg gcgggattca ttaccacagc gagcgggcaa cgaatggcgt ttgtacaata
    42961 tctttctggc tatgcagtag aaccggcgga tcagcgtaat cgccgtattc cgttagtgcg
    43021 ttttgaaagc cgtttgtata aagatattta tcagaacaat tagtcaaaaa gaaaccccgg
    43081 cacatggccg gggttttgct gattaacgct tgtaaatgaa ctcaacgcct tcttcgtcgt
    43141 cttcgtccca gtcgtcatcc cagtcctcat catcctcttc agcaatctct tcaagctgct
    43201 ggcgatgata atcatcccac atgaattcga ctttctctgg ctgtttcgct tcttcagcct
    43261 gcacgaccgg gttttcaatg ataaaggtca tcacatccca gcagagatct ttcacgccca
    43321 gtccactcgc cgcagagatc agataatatt tatcttccca gcccagcgct tcagcgatcg
    43381 ctttcgcttt ctcttcggct tctgccttat ccagcagatc gatcttattg aagactaacc
    43441 aacgcggttt cgccgccaga tcctggctgt atttttccag ctcgctgata ataatacgcg
    43501 cgttttcaac cggatcggtg ccgtcaatcg gatcgatatc gatgaggtgc aacaggacgc
    43561 ggcaacgttc caggtgcttc aggaagcgaa tgcccagacc tgcgccttcc gcagcgcctt
    43621 caatcagtcc tggaatatcg gcaacaacga agctcttttc gttgtccatt cgtaccacac
    43681 ccagacttgg caccagagtg gtaaacggat aatccgccac tttcggttta gccgccgata
    43741 ccgcacgaat aaaggtcgat ttacccgcgt ttggcatccc caacataccg acgtcagcca
    43801 gcagcatcag ctccagcagc agctcacgct tatcgcccgg cgtaccgtta gttttctgcc
    43861 gtggtgtacg gttaactgac gatttgaaac gggtattgcc cagaccgtgc cagccgccct
    43921 tagcaaccag cagacgctga ccgtgtttgg tcatatcgcc catggtttca ccagtaccct
    43981 ggtcgattac acgcgtacct accggcactt taattgtgac gtctttaccg cgcttaccgg
    44041 tacagtcgcg gcttgcgcca ttctgaccgc gctctgcacg gaaagatttt tcaaaacgat
    44101 aatcgataag cgtgttcagg ttctcgtcgg cttccatcca tacgtcacca ccatcaccgc
    44161 cgtcgccgcc atccgggccg cctttcggaa tatacttttc gcggcggaag ctcacgcaac
    44221 cattaccgcc atcacctgca acgaccagaa tcgatgcttc atcaacaaac ttcattttat
    44281 tctccgtaaa tcattcgcct gcgcggggtt gcgataccac cgttgtatgc ttacgtaatc
    44341 cgccccaaat acgatgacca atggcggaat acatcgcgcc cgcaaccacg acaaacgcac
    44401 cgagataacc taaaaggttt aacatcggtc tggcgaagaa atcgggccag gccagtgata
    44461 aaagatctga aaaaaacagc gtaaacagtg gggtgagcgt gatgatcgcg ctcacctgcg
    44521 ctgcctgcca gcgagccatc gcttccgcca gggcgccata tcctaccaag gtattcagtc
    44581 cgcaaaaaat taaacatgcg agctgccagt ggctaagctg cgctatcact ccaggctttg
    44641 ccagagggaa gagcgcaatt gtacataaag tgtacagtaa aaacaggatc tgcggtgagg
    44701 ccagccgacg caataaaacc ttttgcgcca cgccataact cacccaaacc gtcgccgcac
    44761 cgaccccaaa gataactccc caggtgtaat cggttagctt ggtaaatatc tcgaccagac
    44821 tggtgttaaa aaacatcacc aggccgctca ggagcatcaa tgcccctaca acctgagtgc
    44881 tgcgcatttt ctctttcagg ataaatacgc tggcaaccat catgccaact ggcgagagtt
    44941 gcccaatcac ctgcgaagcg gtcggactca ggtattgcaa ggatgagctg aacaggatga
    45001 agttcccaaa cagcccggcg gtcgccactg ccaacaaaat caaccagcgt ggcttacgaa
    45061 acacgcgtaa tggcggcaac ctcttcttca ccgcaagaat ggcacccagg ccaatactcg
    45121 ccatcaagaa acggtaaaac acgattgtcg gaggttccat cacctccagc acctgcttca
    45181 ttgcgattgg caacgccccc cagcaaattg ctgtggtgag cgccaaaaga atgccaatgc
    45241 ctgcctgctg cttcataccg tattccctgc aaagcgcatt tgcggattaa cgtcgcacca
    45301 tgcgcatttt taccggttat cgaatgtaaa aagccccgca acgtgttgcg gggctttcat
    45361 ccgttaccgg gacgcgaaaa acttattcag cttcgatgct gataaattta cggtttttcg
    45421 ggcctttaac ttcgaatttc actttaccgt ctgctttagc aaacagagtg tggtcacgac
    45481 cgcaacctac gttagcgcca gcgtggaatt tggtaccacg ttgacgaacg atgatgctac
    45541 ccgccagaac ggattcgcca ccgaaacgct taacgcccag gcgtttagct tctgaatcgc
    45601 gaccgttacg tgtggagccg ccagcctttt tatgtgccat ttgaaatctc tcctcaggtc
    45661 ttaggcgctg atgccagtaa ttttcacatc agtgaaccac tgacgatggc cctgctgctt
    45721 acgatagtgt ttacgacgac gaaacttaac gattttaact ttctcgccac gaccgtgagc
    45781 aacaacttca gctttgatta cgccgccatc aacgaaagga acgccgattt tgacttcttc
    45841 accgtttgcg atcatcagca cttcagcgaa ctcaacagtt tcgccagttg cgatgtccag
    45901 cttttccagg cgaacggtct gaccttcgct tactcggtgt tgtttaccac cactttggaa
    45961 aaccgcgtac atataaaact ccgcttccgc gcacaccttt tcaatgattc agagtgcgct
    46021 ataaatattc acaatagggc gcgaatatta cgcaaaacgc acgcctttga caagtgctac
    46081 agtcaataca cgaagaaaaa aaacacaact tgtacggtaa cgtttatctg tgccattttt
    46141 tcagtacaat caccctatat tcctaaccat aaaccctaag ttgcctttgt tcacagtaag
    46201 gtaatcgggg cgaaaagccc ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc
    46261 gcgcaagata tggcgggtgt taatgcggca atccttgagc agcttaattc cgacgtccaa
    46321 ctgatcaatc agttaggcta ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg
    46381 attgctgtac tggctgcacg agctgttggc tatgagggaa atgcgcatgt caccattgct
    46441 gccctgatcg agtttatcca cacggcgact ctgctacacg acgacgttgt ggatgaatca
    46501 gatatgcgca ggggtaaagc taccgccaac gccgcatttg gcaatgccgc cagcgtgctg
    46561 gtaggcgatt ttatttatac ccgcgcgttc cagatgatga ccagcctcgg ttcgctcaaa
    46621 gtgctggaag tcatgtcaga agccgtaaac gtcatcgcag agggtgaagt tctgcaactg
    46681 atgaacgtta acgatccgga catcactgaa gaaaactaca tgcgcgttat ctatagcaaa
    46741 accgcgcgtc tgtttgaggc tgccgcgcag tgttccggaa ttctggcggg ctgtacgccg
    46801 aaggaagaga aaggcctgca ggattatggg cgctatctcg gcactgcttt ccagttgatc
    46861 gacgatttac tcgattacaa tgccgatggc gaacagttag gtaaaaacgt cggcgacgat
    46921 ctgaacgaag gtaaaccgac gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa
    46981 caggcacaga tgatccgtac cgccatcgaa cagggtaacg gtcgccatct tctggaaccg
    47041 gttctggaag caatgaacgc ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa
    47101 gaagcagaca aagccatcgc agcgttacag gtgctcccgg acaccccttg gcgagaagca
    47161 ctcatcggcc tcgcgcacat cgctgttcaa cgcgatcgtt aatcccctcc cctcatcccg
    47221 cgcagtgcgc gggatgattc cagtaaactc cataagaata cttattcagc gtaataatgc
    47281 ctgaaaaacc agtgtataca tctttatata ttctgaatat tcacactctt tacaggaact
    47341 ttttagagca atcggccatc aggagtatag tgatgctcga cagaagaagt gttctgaatg
    47401 aaagcgacta acttaaggag tgaggaaaga tggaaagtaa tttcattgac tggcatcccg
    47461 ctgacatcat tgcgggtttg cgcaagaagg gaacgtcaat ggcggcggaa tctcgcagaa
    47521 atggtttgag ttcctcaacg ctggcgaacg cattatcgcg cccatggccg aaaggagaga
    47581 tgattattgc gaaagccctg ggaactgacc cctgggttat atggccatca cgctaccatg
    47641 atccgcagac ccatgagttt atcgacagaa cgcagttgat gcgtagctac actaaaccga
    47701 aaaaatgagt ggtctggcgg tagccccgcg aacggggctg ccagctctca gacgattact
    47761 cgcctttcac acgctcaata tttgcaccta aagcgcgcag tttgtcttca atgcgttcgt
    47821 agccacgatc gatgtgataa atacgatcaa ccaccgtcgt cccttccgca atacagccag
    47881 ccagcaccag gcttgctgat gcacgcagat cggttgccat aacctgtgcg ccagaaagtt
    47941 tttcaacacc gtgacaaata acggtattgc tttcgatttc ggcgtgcgcg cccatacggc
    48001 tcagctctgg cacatgcata aagcggtttt caaaaaccgt ttcggtgatg aatccggtcc
    48061 cttctgccac caggttcaac agcgtgaact gggcctgcat atcggtcggg aatgccggat
    48121 gcggcgcagt acgtacgtta acagccttcg gacgtttgcc atgcatatcc aggctaatcc
    48181 agtcttcgcc gacttcgatg tccgctccag catcacgcag tttcgccagc acggcgtcga
    48241 gagtatctgg ctgcgcgtta cggcagataa ttttgccgcg agagatcgcc gccgccacca
    48301 ggaaagtacc ggtttcgata cgatccggca gcacgcgata gacaccgccg cctaaacgtt
    48361 ccacaccttc gatgacgata cgatcggtgc cctgaccgct aattttcgca cccagcgtaa
    48421 tcaggaagtt cgcggtatcg acgatttccg gttcacgcgc tgcattttca ataatcgtgg
    48481 tgccttccgc cagggttgca gcacacatga tggtcaccgt tgcgccaacg ctgactttat
    48541 ccatcacgat atgtgcgcct ttcagacgac catcgacgga agctttaacg taaccttctt
    48601 ccagtttgat ggtcgcgcct aattgttcga ggccagaaat gtgtagatca accggacgcg
    48661 caccgatcgt acaaccgcca ggtagtgaaa cttgcccctg accaaagcgc gctaccagcg
    48721 gccccagtgc ccagatagaa gcacgcatgg ttttaaccag atcgtaaggt gcgcagaata
    48781 cattaacgtc gcgggcatca atatgcacag aaccattacg ttctactttc gcacccagct
    48841 ggcttagcag cttcattgat gtatcgacgt ctttcagttt cgggacgttc tggatctcta
    48901 ccggttcttc cgccagtaga gcggcaaaaa ggataggcag agcagcattt ttagcgccgg
    48961 aaattgtgac ttcgccctgg agcttcgttg gcccctgaac acgaaattta tccatttagt
    49021 ttgttctcag ttaacaattc atatccgcta ccggcgaatc gcccatagct caaaagccgt
    49081 tcagtttgcg atcgcgcgcc cactccgcag gggtatacgc tttgatcgac acagcatgaa
    49141 tgcggttatc cgcaatatat tccatcagcg gaccatagac cgtctgctgt tttttaaccc
    49201 gactcatgcc gtcaaacaac tcacccacgg caataacctg aaagtggctg ccatcgccgg
    49261 aaacgtggac ttcctggagg gagagagcgt tcatcaacac gctctgaatt tcattatttt
    49321 ccatgggatc ttcaatcatc agttaataaa ccagcgaaac atcttagagc aaagttgcgc
    49381 tggcataaat aagcaaaaag cctcgctgat aaatcagaca aggctcgact tgcaggcagg
    49441 tttgccggac aggcggttaa cgccatatcc ggcctgaaaa aatttaacgg ggcagaacat
    49501 cagcaggcaa attatacaat ttcgccaggg tatacacttt gtcgtttacc ccctgaagcg
    49561 tcacattgtt gccctgcttt ttcgccagat cgataagatg gagcagcagt gccagtccac
    49621 ccgtatccac gcgggacaca cggctaagat cgatgcaggt aacccccttc accgcttcct
    49681 cacgcatttc ccaaagcggt agcagaacgt cctgatccag ctctccggat aacgccagcg
    49741 tgtcacccgt ctgcatccag ctcagtgact cgctcattat tttttctctt ccagagtgat
    49801 tttctgttga gaaatcgatt tcagttgcgc agtcaggccg tcgatacctt tggtacgcag
    49861 cagcgttccc cactcgtttt gtttggtggt gatcatactg acgccttcag caatcatgtc
    49921 gtaagcctgc cagttgcccg tctgggagtt tttacgccac tggaagtcca gacgcaccgg
    49981 cgggcggcca ttcgggtcaa taatggtaac gcgaataggc acaatggttt tatcacccag
    50041 cggctgttct ggcgcaatct gataggtttg accgtgatac atcgccagcg cctgaccgta
    50101 agcctgcttc aggtactcac ggaaagcggc aaagtaggct tcacgttgag ctggggtcgc
    50161 actcttgtaa tactggccca gcaccagcgc accggcgtat ttcacctgta cgtatggcag
    50221 cagttcctga tcaacaatgg tacgcagata atccgggttg gcccgaattt gcggttgctc
    50281 attcttcagg cgatcgaacg ttttctgcgc cgcctcgtcc atcagcttat acggattggt
    50341 ctggtctgcc gcggttgccg cactcagcgg tgcaatcacc agcaaagcga ccatcattaa
    50401 acgtttaaac atgcgtcggt tctcctgaaa ttatttcgtt gtacccacag gttcagtggt
    50461 ttcattatta cctggcgcag cagctggcgc atcgccacta ttcttattgt catcgccttt
    50521 actaccgtaa aggaactgac caatgagatc ttccagcacc atcgcagact tggtgtcctg
    50581 aattgtatcg ccatccttca ggatagcagt ccccagttcc gggtcttcaa aaccgacgtt
    50641 taatgccaga tattgttccc ccagcaggcc ggaagtacga atgctcagcg aactggtatc
    50701 aggaatatgg ttataacgtt gttcaatttc cagcgttacg cgcggcagat aggttttcgg
    50761 gtccagcgta atatccgcca cccggcccac aacaacgcca ccaatactga ccggagagcg
    50821 cgctttcagg ccgccaatgt tatcgaacgt cgcataaagc gtataggtcg gttcagtacg
    50881 tattgacgtc acgttcgccg ccttcaggca aacaaacagc gccgccagca gtgctgctaa
    50941 taaaaagata cccacccaaa tttcattttt tttcgtttgc atgaactcaa ttcccaaaca
    51001 tcaatgcggt cagcacaaaa tccagcccca gaacagccag agacgagtgg acaacggtgc
    51061 gagtggttgc ccggctaatc ccggcagacg tcgggatggc gtcgtagccg ttaaacaacg
    51121 aaatccacgt caccgtgatg gcgaacacca cgctcttaat cagacagttg accagatcca
    51181 tacgccagtc gacggcattt tgcattgccg accagaagaa cccgctatca atgcctttcc
    51241 aactgacgcc gaccagcgat ccgccccaga tccccacggc gacgaagata accgtcaaca
    51301 gtggtaatga aataacccca gcccagaaac ggggagaaat aacccgacgc agcggatcca
    51361 ccgccatcat ctccatactg gagagttgct ctgtagcgcg catcaggccg atttctgcgg
    51421 ttaacgccga accagcacgc ccggcaaaca acagcgcggc aaccaccggc cccagttcac
    51481 gcagtagcga taacgccacc agcataccca gactggtttc cgcactataa gtggtcagaa
    51541 ccagataacc ttgcagcccc aacaccattc cgatgaacac gccagaaact acaataatca
    51601 gcatcgacag gacgccgaca ttatagagct ggcgcaccag cagcggcgca tgtttgcgaa
    51661 attccggttt gccgaccagc gcattgaata acattaaccc ggcccgcccg aacgttctca
    51721 gggttttaat ccctttatgt ccgagcgacg ccagcgcatt taacagcatg agtggcttaa
    51781 ctccctggta aaagatcagc gtgataatcg ccggcaggat agcggaacgg aacaggcccg
    51841 tcagctatcc cgtccagaaa ctgacgtacg cgcggatcag gattcgcctg caacgccttg
    51901 gcactgccat gagcgacaat ttttttgtcc gccaggatcc aggcgtgatc cgcaatactt
    51961 aacacttccg gcacatcgtg agaaaccacc acacaagtca cgcccagcgc gctgttcagc
    52021 tcagaaatca gcttcaccag tacgcccatg gtaatgggat cttgcccaac aaagggctca
    52081 tcaaacatga tgagatccgg ctccagcgca atcgcacgcg ccagcgctgc acgccgcgcc
    52141 atcccaccgg aaagttcaga aggcattagt ttagccgctc cacgcagccc cacggcctcc
    52201 agcttcatca tcaccgtact atgcaacaat ggcgcgggaa gttgggtatg ttcgcgcagt
    52261 ggataggcga cgttgtcaaa tacgttcata tcagtgaaca acgccccgga ctgaaataac
    52321 atgctcatcc gtttgcgcac tgtatacagg cgcgaacgag acatcgccgg aatattctca
    52381 ccatcgaaaa ggatctcacc atgatctggt gcgatttgcc cgccaatcag acggagtagc
    52441 gtcgttttac cgatgcccga tggccccatg atcgccgtga tcttccctcg cggcacggtc
    52501 agggaaatat tatcgaagat gcagcgattg ccacgcgtaa aactgacatc gcgcatatcg
    52561 actaaattcg ccacagactg ctccataatt cacccttcgt cttgcgttga ttttctaagc
    52621 atggcgctca atttaacctt gaacccaaca tatttacaga atattacccg ccgtggttag
    52681 cgaaagctgg catttgtttt actttttagc cgcataaagt caaaattaag aatccgttac
    52741 ggctttctga aaatcttcag cggaccggcg agtatacctg aagaaaggac gttagatgct
    52801 tttagctacg gcactgttaa ttgttggttt acttttggtc gtttacagtg ccgaccgcct
    52861 ggtttttgcc gcgtctattc tttgccgaac ctttggcatc ccgccgctga tcatcggcat
    52921 gacggtggtc agtattggta catcgttacc agaaatcatc gtctcgcttg ccgcgtctct
    52981 gcacgaacaa cgcgatttag ccgttggtac agccctcggc tcaaacatta tcaatatatt
    53041 gctgatcctc ggtctggctg cgctggttcg tccttttacc gtccattctg atgttctacg
    53101 ccgtgaatta cccttaatgt tgttggtcag cgtggtggcc ggttccgtac tctatgacgg
    53161 acaacttagt cgcagcgatg gtatctttct cctctttctg gctgtgctat ggctgctgtt
    53221 cattgttaaa cttgcacgtc aggctgaacg tcaggggact gacagcctga ccagagagca
    53281 gcttgcagag ctgccgcgtg acggcggatt gcccgtcgcg tttttatggc tcggcattgc
    53341 gcttatcatc atgccagtgg ccacgcggat ggtggttgat aacgccacgg tgctggcgaa
    53401 ttactttgcc atcagcgagt tgacgatggg tctgacggca attgctatcg gaaccagcct
    53461 gccggaactg gcaaccgcaa tcgcgggggt tcgcaaaggt gaaaacgaca ttgctgtcgg
    53521 aaatatcatt ggcgcaaaca tttttaatat tgtcatcgtg ttgggtttac ccgcgctgat
    53581 aacgccagga gagattgatc cactggcgta cagtcgtgac tacagcgtga tgttgctggt
    53641 gagcattatt tttgcgttgc tgtgctggcg gcgctccccg caaccgggcc gtggtgtagg
    53701 ggtattatta actggcggat ttatcgtatg gctggcgatg ttgtactggt tatcgccaat
    53761 actcgttgaa taactggaaa cgcattatgt cgcacgtaga gttacaaccg ggttttgact
    53821 ttcagcaagc aggtaaagaa gtcctggcga ttgaacgtga atgcctggcg gagcttgatc
    53881 aatacatcaa tcagaatttc acgcttgcct gtgaaaagat gttctggtgt aaagggaaag
    53941 ttgtcgtcat ggggatgggg aaatcggggc acatcgggcg caaaatggcc gcaacgtttg
    54001 ccagcaccgg tacaccttca tttttcgtcc atcctggtga agccgcgcat ggtgatttag
    54061 gcatggtcac cccacaggat gtggtgattg ctatctctaa ctcaggtgaa tccagcgaaa
    54121 tcactgcctt aattccagtg cttaagcgtc ttcacgtacc gttaatctgc atcaccggtc
    54181 gcccggagag cagcatggcg cgcgccgcag atgtgcatct gtgtgttaaa gtagcgaaag
    54241 aagcctgtcc gttagggctg gcaccgacca gcagcaccac cgccacgctg gttatgggcg
    54301 atgccctcgc tgtcgcgctg ttaaaagcac gcggctttac tgctgaagat tttgcgctct
    54361 cacacccagg cggcgcactg ggtcgtaaac ttctgctgcg cgtaaacgat attatgcata
    54421 cgggcgatga gatcccgcat gttaagaaaa cggccagtct gcgtgacgca ttgctggaag
    54481 ttacccgcaa aaatcttggt atgactgtca tttgcgatga caatatgatg attgaaggca
    54541 tctttaccga cggtgattta cgccgtgtct tcgatatggg cgtggatgtt cgtcagttaa
    54601 gtattgccga tgtgatgacg ccggggggaa tacgcgtgcg ccctggcatt ctggccgttg
    54661 aggcactgaa cttaatgcag tcccgccata tcacctccgt gatggttgcc gatggcgacc
    54721 atttactcgg tgtgttacat atgcatgatt tactgcgtgc aggcgtagtg taaagattca
    54781 aggataaaca acaatgagca aagcaggtgc gtcgcttgcg acctgttacg gccctgtcag
    54841 cgccgacgtt atagcaaaag cagagaacat tcgtctgcta atcctcgacg ttgatggcgt
    54901 actgtcagat ggcctgattt atatgggcaa taacggcgaa gagctgaaag cgttcaatgt
    54961 tcgtgacggt tatggcattc gttgtgcgct cacctctgat attgaagtcg ctatcattac
    55021 cgggcgaaag gctaaactgg tagaagatcg ttgtgccaca ttggggatca ctcacttgta
    55081 tcaggggcag tcaaacaaac tgatcgcctt tagcgatctg ctggaaaaat tggcgattgc
    55141 cccggaaaat gtggcttatg tcggcgatga tctcatcgat tggccggtaa tggaaaaagt
    55201 gggtttaagc gtcgccgtgg ccgatgcgca tccactgttg atcccgcgcg ccgattacgt
    55261 gacgcgcatt gctggcggtc gtggcgcagt gcgagaagtt tgcgacttat tactcctggc
    55321 gcagggcaaa ctggatgaag ccaaagggca atcgatatga gtaaagccag acgttgggtt
    55381 atcattgtgc tatcactggc ggttctggtg atgatcggta ttaatatggc cgaaaaagac
    55441 gataccgccc aggtggtcgt caacaacaat gatcccacct ataaaagcga gcatacggac
    55501 acgctcgtct ataacccaga aggggcgcta agctatcgat tgattgctca acacgttgaa
    55561 tattattccg atcaggccgt ttcgtggttt acgcagccgg tacttaccac gtttgataag
    55621 gataaaatcc cgacatggtc cgtaaaagca gataaagcca agctgaccaa tgaccggatg
    55681 ctctatttat atggacacgt tgaagtcaac gcacttgtgc cggactctca acttcgcaga
    55741 atcacgacgg ataacgcgca gatcaatctg gtgacgcagg atgttacctc tgaagacctc
    55801 gtcacgttat acggaacaac atttaactcc agcggtctga aaatgcgcgg caacttacgc
    55861 agcaagaacg ccgagctgat tgaaaaggtt agaacatcct atgaaattca aaacaaacaa
    55921 actcagcctt aatcttgtgc ttgccagctc acttctggcc gccagcattc cggcatttgc
    55981 cgtaaccgga gacactgatc agccgatcca cattgaatcg gaccagcaat ctcttgatat
    56041 gcaaggcaac gtggttacct ttaccggtaa tgtcatcgtc acccagggca ccatcaaaat
    56101 taatgccgac aaagtggtcg ttacccgtcc gggcggcgaa caaggtaaag aagtgattga
    56161 cggctacggt aaaccggcaa cgttctacca gatgcaggac aacggtaaac ccgttgaagg
    56221 tcacgcttcc cagatgcact acgaactggc aaaagatttt gtcgttctga cgggtaatgc
    56281 ttatctgcag caggtcgata gcaacattaa gggcgataag atcacttacc tggtgaaaga
    56341 gcagaaaatg caggctttca gcgacaaagg caagcgcgta accaccgttc tggtaccgtc
    56401 gcagctgcag gacaaaaaca acaaaggcca gaccccggca cagaagaagg gtaattaatt
    56461 cgttatggca acattaactg caaagaacct tgcaaaagcc tataaaggcc gtcgcgtggt
    56521 agaagacgtc agcctgaccg tcaactccgg ggaaattgtc ggtctgctgg ggccaaacgg
    56581 tgccggtaag accaccactt tctacatggt tgtaggcatt gtgccgcgcg atgcgggcaa
    56641 catcattatt gatgatgacg atatcagtct gctgcctctg catgcacgcg cgcgccgcgg
    56701 tatcggctat ctgccacagg aagcctccat tttccgtcgc ctcagcgttt acgataacct
    56761 gatggcggta ctgcaaattc gtgacgactt gtctgctgaa caacgtgaag accgcgcgaa
    56821 cgagctgatg gaagagtttc acattgagca cctgcgtgac agcatggggc agtcactctc
    56881 cgggggtgaa cgtcgccgtg tagaaattgc ccgcgcactg gctgcgaatc cgaaatttat
    56941 tctgctcgac gaaccgtttg ccggggttga cccgatctcg gttatcgaca ttaaacgcat
    57001 cattgagcac ctgcgcgaca gcggcctggg cgtgctgatc actgaccaca acgtgcgtga
    57061 aacactggcg gtttgtgaac gcgcttatat cgtcagtcag gggcatttga tcgcccacgg
    57121 cacgcctaca gaaatcttac aagacgaaca cgttaagcgt gtataccttg gggaagactt
    57181 cagactctga tagggtagaa gtttgcgacg ttttagcagg agagtacgat tctgaacatg
    57241 aagcaaggtt tgcaactcag gcttagccaa caactggcga tgacgccaca gcttcaacag
    57301 gcaattcgtc tgttgcagtt gtcgacgctg gaacttcagc aggagctaca gcaggcgctg
    57361 gagagtaatc cgctgcttga gcaaatcgac actcatgaag aaatcgacac ccgcgaaacg
    57421 caagacagtg aaacgctgga caccgccgac gcgctcgaac aaaaagagat gccggaagag
    57481 ctgccgctcg atgccagttg ggataccatt tacaccgctg gtacaccatc cggcaccagc
    57541 ggtgactaca ttgacgacga gctgccggtc tatcagggcg aaacgacgca gaccttgcag
    57601 gattacctga tgtggcaggt cgagctgaca ccgttttccg acactgaccg cgctattgct
    57661 acctctatcg tcgatgccgt tgatgacacc ggttatctga ctgtcccgct ggaagatatt
    57721 ctcgaaagta tgggcgatga agagatcgac atcgacgaag ttgaagccgt ccttaagcgg
    57781 atccaacggt ttgatccggt cggtgtggcg gcaaaagatc tgcgtgactg cctgctgatc
    57841 caactctccc aattcgataa aaccacgccg tggctggaag aggccagact gatcattagc
    57901 gatcatctcg atctgttagc caatcacgac ttccgcactt taatgcgcgt cacgcgtctg
    57961 aaagaagatg tgctgaaaga agccgtcaat ctgatccagt cgctcgatcc gcgccccggg
    58021 caatcgatcc agactggcga acctgagtat gtcattccag atgtgctggt gcgtaagcat
    58081 aacggtcact ggacggtaga actcaacagt gacagcattc cgcgtctgca aatcaaccag
    58141 cactacgcct cgatgtgcaa taacgcgcgc aacgatggtg atagccagtt tatccgcagc
    58201 aatctgcagg atgccaaatg gttgatcaag agtctggaaa gccgtaacga tacgctactg
    58261 cgcgtgagtc gctgtatcgt tgaacagcag caagccttct ttgagcaagg tgaagaatat
    58321 atgaaaccga tggtactggc cgatatcgcc caggctgtcg aaatgcatga atcgacgata
    58381 tctcgcgtga ccacgcaaaa atacctgcat agtccacgag gcatttttga actgaagtat
    58441 ttcttttcca gtcacgtcaa taccgagggc ggcggcgaag cttcctccac ggcgattcgt
    58501 gcgctggtga agaaattaat cgcggcggaa aacccagcga aaccgttgag cgacagcaag
    58561 ttaacctctt tgctgtcgga acaaggtatc atggtggcac gccgcactgt tgcgaagtac
    58621 cgagagtctt tatccattcc gccgtcaaac cagcgtaaac agctcgtttg acccaaccga
    58681 taaggaagac actatgcagc tcaacattac cggaaataac gtcgagatca ccgaggcact
    58741 gcgcgaattt gttacagcca aatttgccaa acttgagcaa tattttgacc gaatcaacca
    58801 ggtctatgtt gttctgaaag tggagaaagt cacccacacc tcagatgcaa cactgcatgt
    58861 aaacggtggt gaaattcatg ccagcgcgga aggtcaggac atgtacgctg ccattgatgg
    58921 cttaattgat aagctggcac gccagttgac caaacataaa gataaactga aacaacacta
    58981 attgtccggg caattagcat gtgcatggcg gcctgttgtg cggcacaacg ggccatttgt
    59041 acggttaatg ctccgagcct gttccactgt ttgagtgggc aggttcttag gtgaaattat
    59101 gacaaataat gatacaactc tacagcttag cagtgttctt aacagggaat gtacgcgaag
    59161 ccgcgtccac tgtcagagca aaaaacgcgc cctggaaatc atcagcgagc tggcggcgaa
    59221 acaacttagc ctgccgcctc aggtggtttt tgaagctatc ctgacgcggg aaaaaatggg
    59281 cagtaccggt atcggcaatg gtattgccat tccgcatggc aaactggaag aagatactct
    59341 gcgtgccgtt ggcgttttcg ttcagctcga aacgcctata gctttcgatg ccatcgacaa
    59401 ccaaccggtg gacttacttt ttgccctgct ggtgccggca gaccaaacta aaacgcacct
    59461 gcatactctg tcgctggtgg cgaaacgtct ggcggacaaa accatctgcc gccgtttacg
    59521 cgcagcccag agcgatgaag agctgtatca aatcattacg gataccgaag gtactccgga
    59581 tgaagcgtag ttattcggta atgtctcttt tagacgttgt gaggagaaac agtacatggt
    59641 actgatgatc gtcagcggac gttcaggttc aggtaaatct gtcgccctgc gtgcgctgga
    59701 agatatgggt ttttactgcg tggataacct tcccgtagtg ttgttacccg atctggctcg
    59761 aaccctggcc gatcgcgaga tttctgccgc cgtcagcatt gatgttcgta atatgccgga
    59821 gtcaccagaa atattcgaac aggcgatgag taacctgcct gacgctttct caccgcaact
    59881 cctgttcctg gatgccgacc gtaatacctt aattcgtcgt tacagtgaca cgcgccgact
    59941 gcatccgctt tccagcaaaa acctgtcgct ggaaagtgct atcgacaaag aaagcgatct
    60001 gctggagcct ctgcgttcgc gagcggatct gattgtcgat acctcagaaa tgtccgttca
    60061 cgagctggca gaaatgctgc gtacccgtct gctgggtaaa cgtgaacgcg aactgacaat
    60121 ggtctttgag tctttcggct tcaaacacgg tatccctatc gatgcagatt acgtctttga
    60181 cgtgcgcttc ttgccgaacc cgcactggga tccgaaactg cgtccaatga caggtcttga
    60241 taagcctgtc gccgcgttcc tcgaccgcca cacagaagta cacaatttta tctaccagac
    60301 gcgaagctat cttgagctat ggttacctat gctggaaacc aacaaccgta gctacctgac
    60361 ggtcgccatt ggttgtaccg gcgggaagca ccgttcggtg tatattgcag agcaactggc
    60421 tgactacttc cgctcgcgcg gtaaaaacgt ccagtcacgc catcgtacgc tggaaaaacg
    60481 taaaccatga ccgtcaagca aactgttgaa atcacaaaca agctgggcat gcatgcccgg
    60541 cctgcaatga agctgtttga attaatgcag ggttttgacg ctgaagtgct gttacgtaat
    60601 gacgaaggca ccgaggctga agccaacagc gttattgcgc tgctgatgtt ggattctgcc
    60661 aaaggacggc agattgaagt tgaagcgacc ggtccacagg aagaggaagc actggccgcc
    60721 gttatcgccc tctttaattc tggttttgat gaagattaat cttcatcact ttgacataca
    60781 actcccttca aactcccccc gacaataaga aaatcacgta ctgaaatcgt tctcaatcaa
    60841 cgtcaattgt acatttcgtg cgctttttac tttcagaaga accttaagaa aaccttaaga
    60901 agcattgttt aggtttcgtt taagttaatc gaccatactg gagatcgtca gaaaatattt
    60961 ccaggagatg gcatgattcg cttatctgaa caaagtcccc tgggcaccgg aagacatcgg
    61021 aaatgttatg cgcatccgga agatgcccaa cgctgtatca agattgtcta ccatcgtggc
    61081 gatggtggcg ataaagagat ccgccgtgag ttaaagtact acgcgcatct tggtcgccgg
    61141 ttaaaagact ggagtggaat accgcgctat cacggtaccg ttgagacgga ttgcggaacc
    61201 ggttatgtct acgatgtgat tgccgatttt gacggcaagc cctccattac gctgaccgaa
    61261 tttgctgaac aatgccgtta cgaagaagac gttgcacaac tgcgccaatt actgaaacag
    61321 ctgaagcgtt atttgcagga taaccgtatc gtgacgatgt cgctgaagcc gcagaatatc
    61381 ctctgtcacc gcattagtga gtcagaggtt atccccgtcg tctgcgacaa cattggtgaa
    61441 agcacattga ttccactggc gacctggtca aagtggtgtt gtctgcgtaa gcaagagcgg
    61501 ttgtggaaac gatttatcgc ccaaccggcg ctggctattg ccctgcagaa agatctgcaa
    61561 cctcgtgaga gcaaaacgct ggccctcact tcgcgcgaag cttaatccag ctggtgttgt
    61621 tgcataaacg gctcaccgcc taactgatac atctgccgta aaatccacgc ctgacggctg
    61681 cgcacgtagc ccgatggtgc ggagactttg aaacgaagtg gattaggtaa tacagctgcc
    61741 agtaaggcag cttccgatcg ggtaagtttg ctcgcgggtt tgtggaaata gcgttgtgcc
    61801 gcagcttcga cgccaaacac accgtcgcca aattcggcga tattcaggta aaccgtcagg
    61861 atacgctttt tgctccagac tgtttctatc cccagcgtta atccggcttc cagccctttt
    61921 cgaacccagc tacgcccatc ccataaaaag agatttttgg ccgtctgctg agaaatcgtt
    61981 gaagcaccgc gaatacggtt ttcattgcgc tcgttgtgcg ccagggcttt ctcaatggaa
    62041 gcgacatcaa agccccagtg ctcaggaaat ttctgatctt ctgcggcaat caccgccagt
    62101 cccatccacg gcgagatttg atccatactg acccagtcag aatgtgccac gtaacgaaaa
    62161 ttgccatgca gccaggcgct gacctgtcgc tcgaccatta ccgctgagaa aggaacaggc
    62221 gcaacgctaa acaacgcgat gcccccgccc cagaaaacgg cgaggacaac cattaaccgc
    62281 agaagaaaac ggcgaacgaa actaaacacc gttaagcggc ttttactcat tcagccagaa
    62341 ccagcacgcg ggaaaccagc ttatcaatgc cgctcgccgc ttctgcaatg ttctgcgcca
    62401 gcatatatgc cggggtggtg acaatcttat tgtcttcatc aaccacgata tcatcgacag
    62461 gacacggcac atgctctgcg cccatctctt ccagcacttc tgcggtatcg atatcagtac
    62521 cgatggtcaa acgcagcggg aaatcgaaaa ttttcggcag catcgccggg gcaatacaca
    62581 taaaaccaag cggttttccg gcctgatgca tcgcttgtgc cagcgccttt aattcacggt
    62641 caacggtgca ttcgcgacca agactggcaa aattgcttaa attctttgcc gcgccaaacc
    62701 cccccggcac aatcaacgca tccagttcag cggcatcggc ctgggccaga ggacggattt
    62761 caccgcgcgt tattcgtgcc gcttcaatca gcacattgcg cgtttccgtc atcgcttcgc
    62821 cagttaaatg gttgataaca tcaacctgct gcttatccgg tgcaaagcag accgcctgcg
    62881 caccgctgcg tgaaatagct aacaacgtca acaccgcttc atgaatttca gaaccgtcat
    62941 agacaccgca tccgctcaga attacgccaa ttttcttcat tgtgatcatc ctttcgcaat
    63001 tgactgaaac acattaattt ttttaataaa aatggtacgc atcacacatt taactgattc
    63061 atgtaacaaa tcatttaagt tttgctatct taactgcgtg cggcctgaaa aacagtgctg
    63121 tgcccttgta actcatcata ataatttacg gcgcagccaa gatttccctg gtgttggcgc
    63181 agtattcgcg caccccggtc tagccggggt cattttttag tggcttttgc cacccacgct
    63241 ttcagcactt ctacgtcgtg acgccactct tctttcatct cttcaatcca ttcaccaacg
    63301 ttatcttccc aggcaggaag gtcaggagac tgaatttgct gacccagctg ttgcagatgg
    63361 cgtaacccca ctgaccccgc cgcaccttta attttatgtc cttcctcaac aatgcctttt
    63421 ttatcctgcg ccgtcagatt cgactccagc acgctgacat agcccggcat catcttctca
    63481 aacaccgcta acccgtcggt gatcagcttc ggtcctacaa gttcgagata ctgttccagc
    63541 atgggaatat cgagcaatgc ttctgattta ctgttctctt ctgtcgtcac cgtactctcc
    63601 tcatcatcct gggtatccca gaatttcttg atcatcgcgg ttagcgccgg aacagaaagc
    63661 ggcttactca gcacatcatc cattccagca ttgaggtact cttgcttgtc tttcagcacg
    63721 ttagcggtta aagccaccag cggcggtaaa tcctcgcgcg gataacgctt cgtcagttca
    63781 cgagagatat ccagcccggt catatctggc aactgaatat ccagcaacac caggtcgtat
    63841 tcgcccggtt taaacatctc cagcgccgcc ttgccggtca tggcgacatc aacgctgtta
    63901 cctaattttt ccagcacaga acgcgcgaca atcacgttca gttcaatgtc ttccaccagt
    63961 agcacattca gcgccggtaa aggcatatcg tcttcatcaa acgcatcatc gacctcttct
    64021 gccaccgacg gtgcgtggat cgtcaacgta aaggttgaac ctttgccctg ttcgctggta
    64081 accgtaatat cgccgcccat atttttcgcc agacgacgag aaacggccag accaataccg
    64141 gtgccggtgg caggtttacc gccatgactg tctttcacct ggtaatacat ggcgaagatt
    64201 ttatccagct catcctgcgg aatgccgata ccagaatctt ccacttcaaa atgcagcata
    64261 tcgccttcat cgtagcgcac gcgcacggta acctggcctt gctgggtgaa tttgacggcg
    64321 ttactgatga ggttccacag gatctgccgt aaacgcgtcc cgtcggtaat gacctgatgc
    64381 ggtaatggca gcgtaggctc caggttaaag cgcaatcctt tttgttgcgc ctgcaaggcg
    64441 gagagatttt ccagatcggc aaggaagctg gtgaaatcaa ccggctgatt atcaagctgg
    64501 accttgcgcc gttccatctt atccatgtcg ataatatcgt tgaagatatt ccccagcgtg
    64561 acggccgaaa catggatagt tttgagatat ttttcctgct cggcggtgag ttcggtatcc
    64621 agtaggatgc ggctcaggcc gacgataccg ttcagcggtg tacgcaattc gtgactgatg
    64681 gtggaaataa acgtcgtttt gtcgcggctg gcgcgttcaa gcgcatcctg ataccgctta
    64741 cgctcggtaa tgtcgcgacc aaagcccatc aaaccgtgac gtttacccac gcggtcgtag
    64801 tacggcactt tacgaatttc aaagcaggct ttgcgtccgt cgggataatc cagccactgc
    64861 tcataggtca gtgacacatt atgacggaac actttttcat cggtttcaat gactttcgca
    64921 gcggcttccg gtgagtaaac atcagcaggt ttcagatgaa ccagttgttt ttcgcttttt
    64981 ccggtcagca gctccatcgc gcggttacag ccggaaaact ctttatcttc gttacgataa
    65041 aaaaccaggt cgggtgaagc atcaaggaag gaacgtaaga atgaggattg ctgctcgagc
    65101 tgaatttgtg tctcttcgcg ctctttgatt tcaattttca gttggccgaa ggtttcctgc
    65161 agttctgctt ccgctttttc acgaacggca atttcctgat ttagctgggc aatattatct
    65221 tttaactgaa cgttgaggct caaatcgcgc tcgcgcatct cctccagttt ttgcaccagc
    65281 cgtgacagac gttgtcgtga ctcctccagt tgctcgacga ccaccgatag aaagtagacc
    65341 gcccacggcg taatcagcaa accaaagaag atagaacgaa taacatcaat gctttcgacc
    65401 tgaccatgca gcaccatggt taccgccatt tgtaccacaa tggcaagaac gacgagggcc
    65461 agcgccagca acattgagaa gcgcaccaga cctaacttca tcatcaggtc aacataatac
    65521 tgcgccagca gacgaatttg cttcattagg gaattccttc acgacaacct gccccaataa
    65581 tacccaattc tgacagttac gttataaatt gtgtgagaaa tgcggaattc ccctcaatga
    65641 cctactccgt aggaggtagc cacggacgtc ccagcgccga tccctgtacg ccatgttcat
    65701 tcagatagcg atccagctcg accattcccg tccatcgatt ttcgcaccac agcggagcaa
    65761 gcagcgtcgg gcgacgggcg ctggcggaaa tgcggtggta gatcacttcc ggtggcgtat
    65821 ggcgaatcat ttctccggca gtgagtgtgt aatcctccag ttcaataccg ttcaaacgcc
    65881 ccgcttccca ggctttcgcc ataatgctgc ctttcacaat atgcagcgga tgcagcttta
    65941 tgccgtccac gccggtttca accacgcgct ccagcgtttg caaacattca gcctgccctt
    66001 cgccaggcag gccgacaatt aagtgggaac acaccttcag cccgcgctga cgtgccagct
    66061 gggttgtacg ctgataacag gcaaaatcat ggccgcggtt gatgcgatgc agtgttttgt
    66121 cgtgggcggt ttgtagcccc agctccagcc acacttcgta accctggtcc ttatattcgc
    66181 aaagcagatc cagcaccgca tccggcacgc agtccgggcg ggtaccaaca cacaaaccga
    66241 caatattggc ctggctcacc gcctgctgat acatagaacg cagcacctga acttccgcaa
    66301 aggtgctggt atacgcctga aagtaggcca gatagcgttt agcgcggtta actaaattcg
    66361 cctggtgcgc cagttgctcg gcaatggaac gatgttgctg cgcttcatca gcaaacgagg
    66421 caacattaca gaatgtgcag ccgccacgcc cgatggtacc gtcacggtta gggcagctaa
    66481 aaccgccatg cagcgtcagc ttatgcacct tttgcccata acgacgggtg agatcaccac
    66541 caaacatatt gactaatttc tgtaactgca taatctgata gaccgcgcct tgaaaagagg
    66601 ccaaagcctg ccatttttag ctcatttcgg cgatgacctg gatcaatcgt cccggcctgc
    66661 ctttaccaac tgcataatca atcaaaatta ccgaaatttc atgcataatc acataaatca
    66721 cttttgctta tcttgtgtca gattttttta tctccttatg gattttaggc aaaaacagta
    66781 gcatgaaacg tcattaccaa ttaaggcagt ataaaatgct ggttttctcg tcagttcaag
    66841 gcaggataag ggttaacacg cctttatgac agtcaggaat tggctgtttc tctaacgacg
    66901 ctccttttat ccttaattag aaaatctatt ttaatttcag tcaattaata aagaattttg
    66961 cgctaaagca catttctgta ccaataagct tgccatttga cctgtatcag ctttcccgat
    67021 aagttggaaa tccgctggaa gctttctgga tgagcagcct gctcattata tttatgcagt
    67081 aattgagatc ccctcttcac cgtattaatc gatgcgaaaa ggacaacaag ggggcgaatg
    67141 cgaggcgcgc gtatgacacg caaaccccgt cgccacgctc tttctgtgcc cgtgcgcagc
    67201 ggttcggtag tggggttccc gcagagcctg ggggaggttc acgatatgtt gtacgataaa
    67261 tcccttgaga gggataactg tggtttcggc ctgatcgccc acatagaagg cgaacctagc
    67321 cacaaggtag tgcgtactgc aatacacgca ctggcccgca tgcagcaccg tggcgcgatt
    67381 ctcgccgatg gtaaaaccgg tgacggttgc ggcttgctgt tacaaaaacc ggatcgcttt
    67441 tttcgcatcg ttgcgcagga gcgcggctgg cgtttagcaa aaaactacgc tgtcgggatg
    67501 ctcttcctga ataaagatcc tgaactcgct gctgccgcac gccgcatcgt tgaagaagag
    67561 ctgcaacgcg aaacgttgtc gattgtgggc tggcgtgatg tccccactaa cgaaggcgtg
    67621 ctgggtgaaa tcgccctctc ctctctgcca cgcattgagc aaatttttgt gaacgccccg
    67681 gcaggctggc gtccgcgtga tatggaacgc cgtctgttta tcgcccgccg ccgcatcgaa
    67741 aagcgtctcg aagccgacaa agacttctac gtctgtagcc tgtcgaatct ggtcaatatt
    67801 tataaaggtc tgtgtatgcc ggcggatctg ccgcgctttt acctggatct tgcggacctg
    67861 cgcctggaat cggccatctg cctgttccac cagcgcttct ccactaacac cgtaccgcgc
    67921 tggccgctgg cgcagccgtt ccgctatctg gcgcataacg gtgaaatcaa caccatcacc
    67981 ggtaaccgcc aatgggcgcg tgcgcgtacc tataaattcc agacaccgct tatccctgac
    68041 ctgcacgacg ccgcaccgtt cgtcaacgaa accggctctg actccagttc gatggataac
    68101 atgctggaac tgctgctggc aggcgggatg gatatcatcc gcgccatgcg tctattagta
    68161 ccacccgcct ggcagaacaa cccggatatg gacccggaac tgcgtgcctt ctttgacttt
    68221 aactccatgc atatggagcc gtgggatggc ccggcgggca tcgtgatgtc tgacggtcgt
    68281 tttgctgcct gtaacctcga ccgtaacggt ctgcgtccgg cgcgctacgt catcaccaaa
    68341 gataagctca tcacctgcgc ctctgaagtc ggtatctggg attaccagcc tgacgaagtg
    68401 gtcgaaaaag gccgcgtcgg gccaggcgaa ctgatggtga tcgacactcg cagtgggcgt
    68461 attctgcact cggcagaaac cgatgacgat ctgaaaagcc gccaccctta taaagagtgg
    68521 atggagaaaa acgtccgccg actggtaccg tttgaagatc tgcccgatga agaagtgggt
    68581 agccgcgaac tggacgacga cacgcttgcc agctaccaga aacagtttaa ctacagcgcg
    68641 gaagagctgg actccgtaat tcgcgtactg ggcgaaaacg gtcaggaagc ggtcggttcg
    68701 atgggcgatg ataccccatt cgccgtgctc tccagtcagc cgcgcattat ttacgactac
    68761 ttccgccagc agtttgccca ggtgactaac ccgccaatcg acccgctgcg tgaagcgcat
    68821 gttatgtcgc tcgccaccag cattggtcgt gaaatgaacg tcttttgcga agcagagggc
    68881 caggcgcacc gtttaagctt taaatcgccg attctgctct actccgattt caaacagctc
    68941 acgacgatga aagaggagca ctaccgcgca gatacgctgg atatcacctt tgacgtcacc
    69001 aaaaccacgc tcgaagcgac ggtcaaagag ctgtgcgaca aagccgaaaa aatggtacgt
    69061 agcggcaccg tgttgctggt gctctccgac cggaatatcg ctaaagatcg cctgccggtt
    69121 ccggctccga tggcggttgg cgcgatccag acccgtctgg tcgatcaaag cctgcgctgc
    69181 gatgccaaca tcatcatcga aaccgccagc gcccgcgatc cacaccattt cgccgtgttg
    69241 ctgggcttcg gtgcgacggc tatttatccg taccttgcct atgaaacgct gggccgcctg
    69301 gtagacaccc acgcgattgc caaagattat cgtaccgtga tgctcaacta ccgtaacggc
    69361 atcaacaaag gcttgtacaa aatcatgtcc aaaatgggca tctccaccat cgcctcttac
    69421 cgctgctcga aactgtttga agcggtcggt ctgcacgatg atgtagtggg cctgtgcttc
    69481 cagggggcgg tcagccgcat tgggggggcg agctttgaag acttccagca ggatctgctg
    69541 aatctgtcga aacgtgcctg gctggcgcgc aagcccatca gccagggcgg cctgctgaaa
    69601 tacgtccacg gcggcgaata ccacgcctac aacccggacg tggtgcgcac gctgcaacag
    69661 gcggtacaga gcggcgagta tagcgactat caggaatacg cgaagctggt taatgagcgt
    69721 ccggcaacca cgctgcgcga tctgctggca attacgccgg gtgaaaacgc ggtcaacatt
    69781 gcggatgttg aaccggcaag cgaactgttt aaacgcttcg ataccgctgc gatgtctatc
    69841 ggcgcgttaa gcccggaagc ccacgaggca ctggcggaag cgatgaacag catcggcggt
    69901 aactctaact ccggtgaagg tggtgaagac ccggcgcgct acggcaccaa caaagtgtcg
    69961 cgcatcaagc aggtggcttc cggtcgcttt ggggttactc cggcgtatct ggtcaatgcc
    70021 gacgtcattc agattaaagt cgcccagggc gcgaagccgg gcgaaggcgg tcagttgccg
    70081 ggcgacaaag tcactcctta cattgccaaa ctgcgctatt cggtgccggg cgtgacgctg
    70141 atctccccgc cgccgcacca cgatatctac tctatcgaag acttagcgca gctcattttc
    70201 gacctcaagc aggttaaccc gaaagcgatg atctccgtga agctggtttc cgaaccgggc
    70261 gttggtacca tcgccaccgg tgtggcgaaa gcttatgccg atttaatcac catcgcaggc
    70321 tatgacggcg gcaccggcgc aagcccgctg tcgtcagtga aatacgcagg ttgcccgtgg
    70381 gagctggggc ttgttgaaac ccagcaggcg ctggttgcca atggtctgcg tcataagatc
    70441 cgtttgcagg tcgatggcgg cctgaaaacg ggtgtcgata tcatcaaagc ggcgattctc
    70501 ggcgcggaaa gcttcggctt cggcactggc ccaatggtgg cgctcggttg taaatatctg
    70561 cgtatttgcc acctgaacaa ctgcgccacg ggtgtagcaa ctcaggatga caaactgcgt
    70621 aagaatcact atcacggcct gccgttcaag gtgacgaatt actttgagtt tatcgcccgt
    70681 gaaacccgcg agctgatggc acagcttggc gtaacacgtc tggtggatct gattggtcgc
    70741 accgacctgc tgaaagagct ggacggtttc accgccaaac agcagaaact ggcgctgtcg
    70801 aagctgctgg agaccgccga accgcatcca ggtaaggcac tctactgcac cgaaaccaac
    70861 ccgccgtttg ataacggcct gctgaacgcg cagttgctgc aacaggcgaa accgtttgtc
    70921 gatgaacgcc agagcaaaac tttctggttc gatatccgca acaccgaccg ttctgtcggc
    70981 gcgtcgcttt caggctatat cgcccagacg cacggcgatc aggggctggc agccgatcct
    71041 atcaaagcgt acttcaacgg caccgcaggc cagagtttcg gtgtgtggaa cgcgggcggc
    71101 gtggaactgt acctgaccgg ggatgccaac gactatgtcg gtaaaggcat ggcgggcggc
    71161 ttaatcgcca ttcgtcctcc ggttggttcc gccttccgca gccatgaagc aagtattatc
    71221 ggcaacacct gcctgtatgg cgcgaccggt ggtcgtctgt atgccgcagg ccgcgcgggt
    71281 gaacgtttcg gcgtgcgtaa ctccggtgca atcaccgtgg tagaaggcat tggcgacaac
    71341 ggctgtgaat atatgacggg cggtatcgtc tgtattctgg gtaaaaccgg cgttaacttc
    71401 ggtgcgggta tgaccggcgg cttcgcttac gttctcgatg aaagcggcga tttccgcaaa
    71461 cgcgttaacc cggaactggt ggaagtatta agcgttgacg atttggcgat ccatgaagag
    71521 catctgcgtg gtcttatcac cgagcatgtg cagcataccg gttcccagcg tggtgaagag
    71581 attctggcga actggtcagc cttcgccact aaatttgcgc tggttaaacc gaagtccagt
    71641 gatgtcaaag cactgctggg tcaccgtagt cgtagcgcag cagagttgcg cgtgcaggcg
    71701 cagtaagggg tagctacaat gagtcagaat gtttatcaat ttatcgacct gcagcgcgtt
    71761 gatccgccta agaaaccgct gaagatccgc aaaattgagt ttgttgaaat ttacgagccg
    71821 ttttccgaag gccaggccaa agcgcaggct gaccgctgcc tgtcgtgcgg caacccatac
    71881 tgcgagtgga aatgcccggt acacaactac atcccgaact ggcttaagct cgccaacgag
    71941 gggcgtattt ttgaagcggc agaactgtcg catcagacca acactctgcc ggaagtatgc
    72001 ggacgtgtct gcccgcaaga ccgtctgtgc gaaggttcct gcaccctgaa cgatgagttt
    72061 ggcgcggtga ccatcggcaa cattgagcgt tatatcaatg ataaagcgtt cgaaatgggc
    72121 tggcgtccgg atatgtccgg cgtgaaacag accggtaaaa aagtggcgat tatcggcgca
    72181 ggtccggcag gtctggcgtg tgcagatgtc ctgacgcgca acggcgtaca agcggtcgtc
    72241 tttgaccgtc acccggaaat tggcggcttg ctgaccttcg gtattccggc tttcaaactg
    72301 gaaaaagagg taatgacgcg ccgccgtgaa atcttcaccg gcatgggtat tgaattcaaa
    72361 ctcaataccg aagtgggccg cgacgtacag ctggacgatc tgctgagtga ttacgatgcc
    72421 gtgttccttg gcgtcgggac ttatcagtca atgcgcggcg ggctggaaaa cgaagacgct
    72481 gatggcgtgt acgcagcgct gccgttcctc atcgccaaca ccaaacagtt aatgggcttt
    72541 ggtgaaacca acgacgaacc gttcgtcagc atggaaggta aacgcgtggt agtacttggc
    72601 ggtggcgata ccgcgatgga ctgcgtgcgc acgtctgtac gccagggagc gaagcacgta
    72661 acctgtgcct atcgtcgtga tgaagagaac atgccaggtt cccgccgcga agtgaaaaac
    72721 gcgcgggaag aaggtgttga gttcaaattc aacgtccagc cgctgggtat tgaagtgaac
    72781 ggcaacggca aagtcagcgg cgtaaaaatg gtgcgtaccg aaatgggcga accggacgcc
    72841 aaaggccgtc gccgcgcgga gatcgtggct ggttccgaac atatcgttcc ggcagatgcg
    72901 gtgatcatgg cgtttggttt ccgtccacac agcatggaat ggctggcaaa acacagcgtc
    72961 gagctggatt cgcaaggccg catcatcgcc ccggaaggca gcgacaacgc tttccagacc
    73021 agcaacccga aaatctttgc tggcggcgat atcgtccgtg gttccgatct ggtcgttacc
    73081 gctattgccg aaggtcgtaa ggcggcagac ggcattatga actggctgga agtttaagct
    73141 ctgtcccagc gccgcctcct cagaaaagag tcggcgctga aattctcctt tcgttctgga
    73201 aagcgcctcg cataatctga agaaaaccaa tgtaatacgc acatctggat tgttaccggc
    73261 agatgcccgg cgtatagctg attccattat tcaacactgc gccatataaa gggaaagata
    73321 atggaatcgc tctcagagag aacctcaaca ggctaccagc aaatccacga cggcattatt
    73381 catctggtcg atagcgcccg gacggaaacg gtacgtagcg ttaacgcgct gatgaccgcg
    73441 acatactggg aaattggccg acgaattgtc gaatttgaac aaggtggcga ggccagggct
    73501 gcgtatggtg cgcagctaat caagcgacta tcaaaggatt taagtctaag gtataagcgt
    73561 gggttctctg caaaaaactt acgccaaatg aggctttttt acctcttttt tcaacatgtt
    73621 gaaattcgcc agacagtgtc tggcgaatta acaccattgg gaattccgca gacaccatct
    73681 gcagaattcc catcggcaaa gatttggcag acgctgtctg ccaaatcttt ccccctgccc
    73741 tggtccacct acgtccgttt actttccgtt aaaaacgctg acgcccgcag cctttatgaa
    73801 aaagagacgc tccgctgtgg ctggtctgtt cgccagctag aacggcaaat tgcgacccaa
    73861 ttttatgagc gaacactgct gtcacatgac aaatcagcca tgctgcaaca acacgctcct
    73921 gccgagacgc atattcttcc gcaacaggcg atacgcgatc cctttgtgct cgaatttctg
    73981 gaattgaaag atgaatactc agaatccgat tttgaggagg cgctgatcaa ccacctgatg
    74041 gatttcatgc tggaacttgg ggatgatttt gcctttgttg gtcggcagcg aaggttacgc
    74101 attgatgaca actggtttcg tgtcgatctg ctgtttttcc accgccgttt acgctgcctg
    74161 ctaatcgtcg atctaaaagt gggcaaattc agctatagcg atgccggaca gatgaatatg
    74221 tatctcaact acgccaaaga gcactggacg ctaccggatg aaaatccgcc catcggtctg
    74281 gttctctgtg cagagaaagg agtcggagaa gcgcattatg cactggctgg tttgcctaac
    74341 accgttctgg cgagtgaata taagatgcaa ctacctgatg aaaaacgact cgcggatgaa
    74401 ctcgttcgga cacaggcggt gctagaagaa ggctatagac tccgttaatt tcagcggttt
    74461 cgaattctgc agacactgtc tgcagaatct ttaaaacgaa ctaagtttta agccattaaa
    74521 tcagccttaa ccttcaccac aaccttttta atctcaccag gttcgccgac aacgcatcct
    74581 ggtttatgcg gttctcccgg cataaacacg gcgaacattc ccggttttaa gttgatggtt
    74641 tgctcgtttt caatggcgct gcaaagctgg taatcatctt catggtggaa ctcttcacac
    74701 tggcgcgcag tgcctgccgt gccaaacaga atccgttcct caccttttaa taacagctgg
    74761 atatcaatgt attgctcgtg caattccgct tttttctcga cgggcgattg agtgttaaac
    74821 gtcatgacat tcataaaaat attgtcgccc tgtaattcgt aacgacccgg cgctttttct
    74881 tgcggtctgg cagctaatgc cagcgttaac gcgtcctgta acgcaggatg taacccagca
    74941 gacggtaatg actgtacttc acccatcatc ataatttttc tccctgggcc aacagcgcag
    75001 ccccaagtaa acctgcatca tggcggtagt gcgccgccag taaatcaaca tgaaatgccg
    75061 ctggctcctg cgccagatac gtttccacca gcgccagata cccttctgcc agaccaacgc
    75121 tgccaccgac caccacgcac tgacaatcag ttgtggcttt aatatcagcg atcagccttg
    75181 ccagcacatg tgcggagcgg tgaatcagct gctgcgcctg ctcgtcgccc tgtccggcgc
    75241 gcatgaaaat agttttcgca tccgcgccag ccaactcccc ctgcgctgcc gctgcaatac
    75301 cgcgaccaga agcaatcgct tccacgcaac ctgtgcgtcc acagccgcag actgggccgt
    75361 gtggatcggc aagcgtatgc ccgatgtgcc ccgccagacc gccagggccg gtaagcagtt
    75421 tgccgccgct cactacaccg ccgccgacgc cagtggaaac ggtgataaac accatctcgg
    75481 ttacatcgcc ttccagcgcc tgatactctg cccatgctgc ggcctgcgcg tcgttaatgg
    75541 caatggtcgg caaattggta agttgttcca gcgttttgac taacggaaag tgtaacaatc
    75601 caccaagatt atgcggattc agcgccagca agctgccgtc gcgaataatc ccggttgaag
    75661 cgatggcaac ccgctgcgca tgagcttgca acggagagac taatgcggat aaggcatcac
    75721 gcagtgcctg cggagtctgg ctggcaggcg taggaagttc tcggcgatcg cggatctgcc
    75781 cgtcagcgcc aatcagcgcg gcggcaagtt tagtaccgcc gatatcaatc gccagcgtgg
    75841 tcatagcacc gcctttttca tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt
    75901 aatggcagaa ccgaccgtca ccgcccacgc gccgtggcgc atcgcatcag ccgcctgagc
    75961 aggcgtgttg taacgccctt cggcaatcac ccgacatccg gcgtcgctca accttttcac
    76021 cagcgtcaga tccggctctt ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc
    76081 aataatttcg gctcccagct tttggcatgc caggccgtct tccggcgttg agcagtcggt
    76141 catcgccagt aaaccgtgat ggtggatacg tgccagcagc atttcaacag gcaccggacg
    76201 cgggcggtcg gtgccgtcaa tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac
    76261 atcttcaata taggccgtga tgcgtaccgg agaatcctcc agatcgcgtt tcacaattcc
    76321 aataatcggc acgctcacca ccgcacgcgt ggcttgcaga tttgccacac cttcgatgcg
    76381 aatggcaacc gcgcccgcct gctctgccgc taatgccatg gctgcgacga tttcgggttt
    76441 atcgagcggg ctgtcaggaa ccggctggca ggagacaatc aggccaccgt tagcagcgat
    76501 tttttgatcc agttgtgcaa gtaacgacat acatcttccc ttagcgaaag gcccggtaca
    76561 tagaccgggc aacaggatta acttttggtt ttgactaaat cgtttttggc gctgccaaat
    76621 ggcacggcac cgctaaatgg tttgccgtcg atagcgtcat gagtacgcaa tgcttccggg
    76681 cgcagccaac gctgaacgcg ggaaggcata tccagaccaa tcagcaggat caccacaaat
    76741 gtcagactga acgagagcga tgccagcgca gtacccagat ccagacgttg agcgatcaac
    76801 gcgccgatga ttggggccag tgcgccaccc aatgcgccaa cgttataggt aaagcccagg
    76861 cccgctgcac gctggtcggt atcgaaataa ccgccaatca gtttcggtaa gatcccggcg
    76921 atcccttgtc caagcatttg ctggaagaac agtaacagac cgagcaccca gacgtttgca
    76981 ccgccaatcg caaataccgg aataatcagc agctgcgagg ccaacagact acaaacgtac
    77041 gctttgcggg ttcccagcca gtcaccgagg aagccaccga cgcagcaccc caccgccgcg
    77101 ccaaagccac taaagaacag cacattggcg acagtatgcg ggttataagc cagatcggtt
    77161 ttcagatacg ttggcagcag cgcctgaatc ggccatgagt agaggaaagc aaacaacacg
    77221 accaccatca gcattacgcc agttggccag cgtttgcccg tactctgcac cataaagctg
    77281 ataaagattg cggcgcataa cagcccaaga acagcgacga tcgcggcatt ttgcaggtta
    77341 ccggcgaagc agaaccacag cgcagtagcc gccgccagtg tcattacgat attggcaatg
    77401 cgatgttcac cacggtagag aatatccacc attgtgcgta ctggtgcttt acctgcgtgt
    77461 ttctctttcc agtcttccgc ttccgggatg tttttacgca gccagagagc aaagataatt
    77521 ggcaaaatgc cgataaagaa cagcgcacgc cagccccaga ccggaaccac caggctgtag
    77581 acctgagcgg caacgacggc ccccacagag aagcctgaaa tcagaaagcc actggctttg
    77641 ttacgcagat gttttggcca gctttcaatg acataggtgg cgctggaacc gtattcaccc
    77701 gccatcccca tgccgatgac cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag
    77761 ccgcaggcca gcgtcccggc cgagaagaga acgatgctgg tgaccattgc cagacgacgc
    77821 ccgtagcggt cacccatagc gccgagcatc aggccgccga accagcgaga gataaaggct
    77881 gcggagatca gacttgccgc ctgcaccgtc gtcagcccga attcaccttg tacttcggtg
    77941 agtaccaggg cgattaaaac gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg
    78001 gaaaatgccc gccattgtgc acgattgaga tggcgatacc acgggatatt ctgggttgta
    78061 gtactcattg tgagtctccc gcggtgggcg atgcccacac gctttggtat gaaaattgta
    78121 gggtacagat gcgtttattt cccctcaccc ggtaggggcg agcgagggga aacaactcac
    78181 ccgcgctctt gcatcaactg ctgggccagc gccttcagtt ctggcagata tttttcatct
    78241 accggaccaa acggtttgcg gcacagcggc acagacacga catccatata atgcagaaca
    78301 gttttcaggc cgcggaatac gcccgttttg atcagtaaat caatgacttt attgcactca
    78361 gtttgcagtt tctgcgcggt ctggatatcg ccttctttca gcgccttcac gatcccctga
    78421 tagcgccagc ccatgatgtt gtaagtactg ccgataccac catcagcgcc tgccagcaag
    78481 ccggaagcga agatttcgtc gtaaccgtta tagagcacca gatcaggatg ttcacgacgg
    78541 atctgttcca tctgatagag atcgccagaa gtctgtttca gcgcaccgac gccaggcaac
    78601 gtaacaagtg tgttgatctg atccagggtc agttttaccc cactcagggc tggaatgttg
    78661 tacaccacca tcggcaaccc atccgctgaa tcaataattg cccgatagtg atcgcagtgt
    78721 tcttcaaagc tgaaaggata gtagaacggc gtgacggcgg agacggcatc gaagccataa
    78781 cgtttagccg atgccgcaag ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg
    78841 atgagtttaa tcttaccttt cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg
    78901 gaaaggcttt gtacaaaggc ttcgccggtc gaaccaccca cgtataaacc gtcaatgccc
    78961 tgctgaatat tgaactgaac caggcggcgc agactcgctt tatccagtgc ttgttgttga
    79021 tcaaaaggag tcaggagtgc agccattacg ccacgtaaat tcgttgccat aaatacctct
    79081 gaagtgatgc ttgtctgata aacgatatac ctttatacct gttataccag atcaattaag
    79141 caacacccca tacagaaagc tgataatgcg atctgcttca ctaaagtggc attatttctt
    79201 tttgttggtg gtctgaccga aggcgtgcca ggtagcagag acgctgttga gatgcgattg
    79261 caacgcacga tcggcttcgt caggatcatg acgccggatc gcgtcaacga tcgcaatatg
    79321 ctgttgatag ctaacgttgt tatgctcgtg cagagcctga tcagccacgg ttggacgtgc
    79381 ggcgatcagc cagtcgagca gggcaacgtg gatcgccatg aagattgggt tacctgggat
    79441 ctccgccagc acgcggtgga aatcaacgtc tgagcgaatg aatgccgcgt tgttatccag
    79501 cgactgactg ttgatttcca gtgcttttgc cagcaaatcg atttgctcat cggtggcgtg
    79561 ttcagccgca tagcgcacca ggctggattc aaagaacaga cgtaattgtt cgaaatgggc
    79621 aatcccacca ggatgagaaa ggaaatcttt cgccatgccg gaaagctcac cgatgatggt
    79681 gtccgcagaa ggacgcgaga cgcgagcgcg ttcgccgttg tttatttgca ccagaccttt
    79741 gcgttttaac gctgccagcg cttcacgcac cgaaggacgc ccgacgttaa agaacgccat
    79801 cagttcgcgt tcagacggta attgttcacc ttcgccaaat tcacgacggc ggatcatctg
    79861 ttccagctct tcttccacca tttcggagag ttttttacgc gccagcgggc ggctacgcaa
    79921 gttgcgacca attgcaggtg aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc
    79981 cattctgtaa ggtcagtgtg attaacatca tcagtgacat cctatcacag gattgaaagt
    80041 aggggaaaat ggcagggttt tctctttgtg cctcatcatt accataatta acggaataat
    80101 taactattgc gaaaaattaa tgtaacgcag ataaaaacat cccgattgaa ttatttacaa
    80161 gactattcac gagcattatg aatattatga atgtgttctt acaaaataat cataagcgca
    80221 tattttttaa tgaaaaatca cctcacctac aattaaaaac acgacatcag caccataaat
    80281 agacttgcaa aaaatataac atcattgttt tcaatctgcc atttatggga ttgaccgttt
    80341 tcttttgaca cggagttcaa caatgttcgg cataattata tctgtcatcg tattaattac
    80401 gatgggctat ttgatcctga aaaactacaa acctcaggtg gtgctggctg ccgcaggtat
    80461 cttcctgatg atgtgcggtg tctggttagg gttcggtggt gtactcgatc ccaccaaaag
    80521 cagcggctac ttgatcgtcg atatttataa tgaaatcctg cgcatgctgt ccaaccgcat
    80581 tgccggattg gggctgtcga ttatggcggt gggcggttat gcccgctaca tggagcgcat
    80641 aggggccagt cgcgcgatgg tgagcttgtt aagccgcccg ttaaaactca ttcgctcgcc
    80701 gtatattatt ctgtcggcaa cttacgtcat cggccaaatc atggcgcagt ttattaccag
    80761 cgcctccggt ctgggtatgt tgctgatggt caccttattt ccgacgctgg tgagtctggg
    80821 agtaagtcgc ctctctgcgg tggcggttat cgcaaccacg atgtccattg agtgggggat
    80881 tctggaaacg aactccattt ttgcagccca ggtcgcggga atgaaaattg ccacttactt
    80941 cttccactac cagcttccgg tcgcctcttg cgtcattatc tcggtggcga tctcccactt
    81001 tttcgtgcaa cgcgcttttg acaaaaaaga taaaaatatc aatcacgaac aggcagagca
    81061 aaaaactctc gataatgtcc cgccgctcta ttacgccatt ttacctgtga tgccgttaat
    81121 cctgatgctc ggctcgctat tccttgccca catcgggctg atgcagtcag aactgcatct
    81181 ggtggtggtg atgttactga gtttgacggt gacgatgttt gttgagttct tccgcaagca
    81241 taaattacgc gaaacaatgg acgatgtgca ggcgtttttt gacggcatgg gtacgcagtt
    81301 tgccaacgtg gtaacgctgg tggtcgcggg tgaaatattt gcgaaaggct taacgacgat
    81361 tggcactgtc gatgcggtta tcaggggtgc ggagcattct ggtctgggcg gtattggcgt
    81421 gatgattatt atggcgctgg tcattgccat ttgtgccatt gtgatgggct ctggcaatgc
    81481 gccgtttatg tcatttgcca gtcttattcc gaatatcgca gccggactac atgtaccagc
    81541 ggttgtaatg attatgccga tgcattttgc cacgacgcta gcgcgcgcgg tttcgccgat
    81601 tactgcggtg gtagtcgtta cgtcaggaat tgcaggcgtt tcgccttttg cggtggtgaa
    81661 gcggacagcg atccccatgg cagtcggttt cgtggtgaat atgattgcca caatcacgct
    81721 attttattaa gccattaaaa gacaaaacag gccaccaggg cctgttttgt attacttaac
    81781 aacgcgtaat gccggtcgac caccgcgtgg tggctgcgga ggttcatcgt caggatgagt
    81841 gtcatcatcg tgatctggct tgtcgccatc aataaccgac ataacggttt cgttgtctgc
    81901 cgatgcctct tcatcattca tgatgctggc atcttcatcg taggcagctt caggttcaaa
    81961 catcgtgcca gcgccatttt cacgggcgta gatagccagc acggcagcca gcggcacaga
    82021 aacctgacgc ggaatgccac caaagcgcgc gttaaagcgc acctcatcat tcgccagttc
    82081 cagattgccg acagcacgcg gcgcaatgtt gagtacgatt tgcccgtcac gcgcatattc
    82141 cataggaacc tgcacgccag ggagcgtcac atccaccacc aggtgcggcg tgagctggtt
    82201 atccagcaac cactcataga atgcacgcag cagataggga cgacgtggtg ttagctgtga
    82261 caaatccata cagattaact ccggcccaga cgcatttcac gttctgcttc agttaaagaa
    82321 gcaaggaaag agtcacgctc aaagacgcgg gtcatatagc ctttcagctc tttcgcaccc
    82381 gggccgctga actcgatgcc cagttgcggc agacgccaca gcagcggagc aaggtagcaa
    82441 tcgaccaggc tgaactcatc actcaggaag tacggcttct gaccgaagac cggcgcaatc
    82501 gccagcagtt cttcgcgcag ttgcttacgt gcggcatctg cttcagaagc tgaaccgttg
    82561 atgatggtgt tcatcagcgt gtaccagtct ttttcgatgc gatgcatgta cagacggctt
    82621 tcaccgcgag ctaccgggta aacaggcatc agtggcggat gcgggaaacg ctcatccaga
    82681 tattccataa tgatgcgaga ttcccacagg gtcagctcac gatccaccag ggtcggaacg
    82741 ctctgattcg ggttgaggtc aatcagatcc tgaggcggat tgtccttttc cacgtgttcg
    82801 atctcgaaac ttacaccttt ctcagccagc acaatgcgga cctgatggct atagatgtca
    82861 gtaggaccgg aaaacagcgt cattaccgaa cgtttgttgg cagcgacagc catgaaaacc
    82921 tccaggtata gtcagaattt ttactgctac cagccaccag gtggccagtc agaagttgtg
    82981 ttacccagta aggaacgact ctctttgttc gaaaatcaaa caaaaaatga gcaatacccg
    83041 acatttgggc agaaaattgg atgatagttt accagatttt gcgacctttg tggtgagtcg
    83101 atgccggaaa tggggaaaaa gagatgcgct ttagtctgaa atagttgact tagcccctta
    83161 ttggcgatgt ggtttttgtt ttgcctgtct gtcagacggc agcaaaaagc aactttccat
    83221 tttttacgct aattcaaaat ttagccataa aaaaacccgc cgaagcgggt tttttcgcaa
    83281 attgttttct gccggagcag aagccaatta acgtttggag aactgcggac gacgacgtgc
    83341 tttacgcaga ccgactttct tacgttcaac ctgacgagcg tcacgagtaa cgaagccagc
    83401 tttacgcagt tcagaacgca gggactcgtc gtattccatc agagcgcggg tgataccgtg
    83461 acggatcgca ccagcctgac cagagatacc accaccttta acggtgatgt acaggtccag
    83521 tttctcaacc atgtcgacca gttccagcgg ctgacgaact accatgcggg cagtttcacg
    83581 accgaagtac tgttccagag aacgttggtt gattacgatt ttaccgttgc ccggtttgat
    83641 gaaaacgcga gctgcggaac ttttgcggcg accagtgccg tagtattgat tttcagccat
    83701 tgcctataat cccgattaga tgtcaagaac ttgcggttgc tgtgccgcgt ggttgtgctc
    83761 gttacccgcg taaactttca gtttacggaa catagcacga cccagcgggc cttttggcaa
    83821 catgccttta accgcgattt caatcacacg ctcaggacgg cgagcaatca tctcttcaaa
    83881 ggtcgcttgt ttgataccac cgatgtggcc ggtgtggtga tagtacactt tgtcagtacg
    83941 cttgttgccg gttacagcaa ctttgtcagc gttcagaacg atgatgtaat caccggtatc
    84001 tacgtgcgga gtgtattccg ctttgtgctt accgcgcagg cgacgagcca gttcagtagc
    84061 cagacggccc agagttttac cggtcgcgtc aacaacatac cagtcgcgtt ttacggtttc
    84121 tggtttagct gtaaaagttt tcattaaaag cttacccaat aaatagttac acgttggtga
    84181 acacccaaac gtcttcaatt gttgaggttc acacgacaaa gtccggcaaa cctacccctt
    84241 cgaatagcct atgccagcac acaaaaagtt ttgggaaaaa aactttcttg taacgtgggg
    84301 tcgcaggatt atagagaagt cggggtcaaa gatcgacccc tttttgtgat ttgtggcagg
    84361 ttttaacccg ccaaatgctc gcgcttcaga tactcttcgc tttgcatctc ttgcagacgt
    84421 gacaggcaac gctggaactc aaacttcagc cgctcaccct gataaatttc atacagcggc
    84481 acttctgcac tcaccactaa tttgacatgg cgctcgtaaa actcatccac cagcgcaata
    84541 aagcgccgcg cttcgctctc catcaaccgc gtcataactg gtacatcaaa caacatgacc
    84601 gcatgaaaga gacgtgagag cgcaatatag tcatgctgac tgcgggcgtc gacgcacagc
    84661 gtagtaaaag agaccgccag cgtctggttc tcgacgccca ttgtcgctaa tggccgatgg
    84721 ttgatttcta acgttggtga aatttctcgt ttcgcccccg ccagcgccaa ccatagttta
    84781 tccatttgcg cccggatttc atcgttgagt ggcgaaagcc acagatgcgc ctgagtgagt
    84841 gtacgcagtc gataatcaac accagcgtcc acgttcatta catcacaatg ctgtttaatg
    84901 gcatcgattg caggcagaaa acgcgcacgt tgcaggccat ttcgataaag ttcatccggc
    84961 ggaatatttg acgtcgctac cagggtaata ccgcgggcga acagggcttt catcagaccg
    85021 ccaagtagca tggcgtcggt aatatcagaa acaaaaaatt cgtcaaaaca gagcacgtca
    85081 gtttcggctt taaagcgatc ggcaataatt tccagcggat cggtctgccc ctgtaaggca
    85141 gttagctctt cgtgcacccg cagcataaaa cggtgaaagt gcaggcgctg tttccgctct
    85201 cccggcaggc tttgatagaa aaggtccatc agccaggttt tcccgcgtcc tacaccgccc
    85261 cacatatata agccacgcac tggcgtatgc tttgtgtctt cgcgtttacc ccacagctta
    85321 ccgacccgcg ccattagccc actcgtcctg ggggctggtg gcctgctatt gatgagttcc
    85381 tgataaataa tttccaggcg gctgacggcc tctttttgaa cgtcgtcggg ttgatggctg
    85441 ccttcattga gcgccttcag gtattgcgat gttggggtaa cgctttgcat gatcttattg
    85501 ttattccttg aataatcggt gcgccgttgt tcacggttga cgaaaaaaag gccgttctac
    85561 actacgcgat atgcagtcgg gattccactt ctgtggcatt aacggttata gtggcataat
    85621 cagccgcagg catggagcct gaagccaaca ccctacggaa acaaaagaca acgggagatg
    85681 ttcatgacct gggaatatgc gctaattggg ttagtcgtcg gcatcattat tggtgctgtg
    85741 gccatgcgtt ttggtaatcg taaactacgc cagcaacagg cgttgcagta cgaactggaa
    85801 aagaataaag ctgaactgga cgagtatcgc gaagagctgg ttagccactt tgcccgcagc
    85861 gcggaattac tggataccat ggcgcacgac tatcgccagc tgtatcagca catggcgaaa
    85921 agctccagca gcctgctgcc ggaactgtct gctgaagcaa acccgttccg taatcgtctg
    85981 gcagagtctg aagccagcaa cgatcaggca ccggtgcaga tgcctcgcga ctattctgaa
    86041 ggcgcatccg gcctgctgcg tactggcgcg aagcgcgact aatttatttt tcgggcgcag
    86101 ccattgcgcc ctcctcttct ctccctcccc gactatcatt taatctggtg tctcattgtt
    86161 agccgtctga aaattcaata acatcaaact gttttgaatc tcttttctta tcattcaggt
    86221 acgagagcag gaataatgaa aaaacaaacc cagctgttga gtgcattagc gttaagtgtc
    86281 gggttaactc tctcggcgtc atttcaggcc gtcgcgtcga ttccaggcca ggttgccgat
    86341 caggcccccc tccccagtct ggctccaatg ctggaaaaag tgcttccggc agtggtgagc
    86401 gtacgggtgg aaggaacggc cagtcaggga cagaaaatcc cggaagaatt caaaaagttt
    86461 tttggtgatg atttaccgga tcaacctgca caacccttcg aaggtttagg ctccggtgtc
    86521 atcatcaacg ccagtaaagg ctatgtgctg accaacaacc atgtgattaa tcaggcacag
    86581 aaaatcagta ttcagctcaa tgatgggcgc gagtttgatg caaaactgat tggtagcgat
    86641 gaccagagcg atatcgccct gttgcaaatt caaaacccga gcaaattaac gcaaatcgct
    86701 attgccgact ccgataaatt gcgcgtcggt gattttgccg tggcggtcgg taacccattt
    86761 ggccttgggc aaaccgccac ctctggcatt gtttccgcat taggacgcag cgggttgaat
    86821 cttgaaggtc tggaaaactt tatccagaca gatgcttcca ttaaccgcgg taactccggc
    86881 ggtgcactgt taaaccttaa cggtgagtta attggcatca acactgcaat ccttgcgcct
    86941 ggcggcggga gcgtcggaat tggatttgcc atccccagta atatggcgcg aacactggcg
    87001 cagcagctta tcgactttgg tgaaatcaaa cgcggtttgt taggcatcaa aggcaccgag
    87061 atgagtgccg atatcgccaa agccttcaac cttgacgtgc agcgtggcgc gtttgtcagc
    87121 gaagtgttgc caggttctgg ctcggcgaaa gcgggcgtca aagcgggcga tattattacc
    87181 agcctcaacg gcaaaccgct gaatagcttt gctgagttgc gctctcgtat cgcgaccacc
    87241 gagccgggca cgaaagtgaa gcttggcctg ctgcgtaacg gcaaaccact ggaagtagaa
    87301 gtgacgctcg ataccagcac ctcttcgtcg gccagcgctg aaatgatcac gccagcgctg
    87361 gaaggtgcaa cgttgagcga tggtcagcta aaagatggcg gcaaaggtat taaaatcgat
    87421 gaagttgtca aaggaagccc agctgctcag gctggcttgc aaaaagacga tgtgatcatt
    87481 ggcgtcaacc gcgatcgggt gaactcgatt gctgaaatgc gtaaagtgct ggcggcaaaa
    87541 ccggccatca tcgccctgca aattgtacgc ggcaatgaaa gcatctatct gctgatgcgt
    87601 taatgtcgta aaccgggcat caggctgacg tgtgatgtcc ggttaactcg tggtatgctg
    87661 ctgccgttcc cttttttaat gacgcctcca tcatgtttgt gaagctctta cgttccgttg
    87721 cgattggatt aattgtcggc gctattctgc tggttgccat gccttcgctg cgcagcctta
    87781 acccgctttc cactccgcaa tttgacagta ccgatgagac gcctgccagt tataatctgg
    87841 cggttcgccg cgccgcgcca gcggtggtta acgtttacaa ccgtggtttg aacaccaact
    87901 ctcacaacca gcttgagatc cgcaccctgg gatccggtgt aatcatggat caacgcggtt
    87961 atatcatcac caataaacac gtcatcaacg acgccgatca gatcatcgtc gccttacagg
    88021 atggacgtgt atttgaagca ttgctggtgg gatctgactc tctaaccgat ctggcggtac
    88081 ttaaaattaa tgccactggc ggtttaccta ccattccgat caatgcacgt cgcgtaccgc
    88141 acattggcga cgtggtactg gcgatcggta acccgtacaa cctcgggcag accattaccc
    88201 aggggattat tagtgccacg ggtcgaatcg gtctgaaccc gaccgggcgg caaaacttcc
    88261 tacaaaccga tgcttccatt aaccacggta actctggcgg cgcgctggtg aactcgctgg
    88321 gagaactgat gggcattaac acgctgtcgt ttgataagag taacgatggc gaaacgccgg
    88381 aaggtatcgg ctttgcgatt cctttccagt tagcaaccaa aattatggat aagctgatcc
    88441 gcgatggtcg cgtgatccgc ggctacattg gtatcggcgg acgcgagatc gcaccactgc
    88501 acgcgcaggg cggtggtata gatcaactgc aagggatcgt ggttaatgaa gtgtcacctg
    88561 acggcccggc ggcgaatgcg ggtattcagg tcaacgatct gattatttcg gtggataaca
    88621 aaccggccat ctctgctctg gagacgatgg atcaggtggc agaaattcgc cctggctcgg
    88681 tgatcccggt tgtagtgatg cgtgatgata agcagttaac gctgcaggtc accattcagg
    88741 aatatccggc aaccaattaa gtcgagtgct caaaacaaaa aaccggagtc tgtgctccgg
    88801 ttttttatta ttcgctaatc aattacttat taacgaactc ttcgcccagg gcgatatctt
    88861 tcttgagcgt atccagcata ccttccagcg cgttctgttc aaatgcgctc agggtaccga
    88921 tagatttacg ctcttccacg ccgtttttac ccagcagcag cggttgagag aagaaacggg
    88981 cgtactgacc gtcgccttca acgtaggcac attcgacaac gccttgttcg ccctgcagtg
    89041 cgcgaaccag agacagacca aaacgtgcag ctgcctggcc catagacagg gttgcagacc
    89101 cgccaccggc tttcgcttca accacctcag tacccgcgtt ctggatacgt ttggtcagat
    89161 cagccacttc ctgctcggta aaactaacgc caggaacctg tgacagcagc ggcagaatgg
    89221 taacaccaga gtgaccgcca ataaccggca cttcaacttc gcctggctgt ttgcctttca
    89281 gttccgcaac aaaggtgttg gaacgaatga tatccagcgt ggtaacgcca aacagtttgt
    89341 ttttgtcata aacaccggct tttttcaaca cttcagcagc aatcgcaact gtggtgttaa
    89401 ccgggttagt gataatacca atgcacgctt tcgggcaggt tttcgcaact tgctgtacca
    89461 ggtttttcac gatgccggcg ttaacgttaa acaggtcgga acgatccata cccggtttac
    89521 gcgctacacc tgcagagata agaacgacat ctgcgccttc cagcgccgga gtcgcatctt
    89581 caccagaaaa acctttgatt ttcacagcag tagggatatg gctcagatcg acagccacac
    89641 cgggagtcac tggagcgata tcatacagag agagttctga acctgaaggc agttgggttt
    89701 ttaacagtag tgcaagcgcc tggccaatac cgccagcagc gccgaggact gcgactttca
    89761 tcctaaactc cttattatat tgataaacta agatatgttg ctccgctgcc gcgaccttaa
    89821 tccacaaaat tgccgtttac aatgaccaca tctcaagaat gtgtagtcac gcaagtttag
    89881 cgtttatgca tttaattgcc gtaatcagga acctaactta cgtaattaac agtcgcttat
    89941 gcattagcgc aacattccaa caggtggtga caatataccc taccgttcag ccaaaacaac
    90001 atcaatttga taacaattaa tttactttta agcagaattt gcatgccgtg acgcaggcat
    90061 gtttctcaat aacgaaattt gataaaatcc cgctctttca taacattatt tcagccttct
    90121 tcagggctga ctgtttgcat aaaaattcat ctgtatgcac aataatgttg tatcaaccac
    90181 catatcgggt gacttatgcg aagctcggct aagcaagaag aactagttaa agcatttaaa
    90241 gcattactta aagaagagaa atttagctcc caaggcgaaa tcgtcgccgc gttgcaggag
    90301 caaggctttg acaatattaa tcagtctaaa gtctcgcgga tgttgaccaa gtttggtgct
    90361 gtacgtacac gcaatgccaa aatggaaatg gtttactgcc tgccagctga actgggtgta
    90421 ccaaccacct ccagtccatt gaaaaatctg gtgctggata tcgactacaa cgatgcagtt
    90481 gtcgtgattc ataccagccc tggcgcggcg cagttaattg ctcgcctgct ggactcactg
    90541 ggcaaagcag aaggtattct gggcaccatc gctggcgatg acaccatctt taccacccct
    90601 gctaacggtt tcaccgtcaa agacctgtac gaagcgattt tagagctgtt cgaccaggag
    90661 ctttaatctc tgccccgtcg cttctggcgg cggggaaaat gttgcttatc cctctcaacc
    90721 ccctgctttc ccctgcgatt aatttaacga atagtgcgtt ttactgcgac atgtcattca
    90781 cacaatgaat acatacggta aaaaaagc
//
LOCUS       DAZFKF010000005        88310 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFKF010000005 DAZFKF010000000
VERSION     DAZFKF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 88310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.005"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            2..169
                     /gene="yhfL"
                     /locus_tag="ACHKA1_000437"
     CDS             2..169
                     /gene="yhfL"
                     /locus_tag="ACHKA1_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO5678141.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            465..1802
                     /gene="frlA"
                     /locus_tag="ACHKA1_000438"
     CDS             465..1802
                     /gene="frlA"
                     /locus_tag="ACHKA1_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO5678142.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAVTAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1823..2845
                     /gene="frlB"
                     /locus_tag="ACHKA1_000439"
     CDS             1823..2845
                     /gene="frlB"
                     /locus_tag="ACHKA1_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO5678143.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGEQASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2895..3722
                     /gene="frlC"
                     /locus_tag="ACHKA1_000440"
     CDS             2895..3722
                     /gene="frlC"
                     /locus_tag="ACHKA1_000440"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO5678144.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDE"
     gene            3722..4507
                     /gene="frlD"
                     /locus_tag="ACHKA1_000441"
     CDS             3722..4507
                     /gene="frlD"
                     /locus_tag="ACHKA1_000441"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO5678145.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIKPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPELVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4607..5338
                     /gene="frlR"
                     /locus_tag="ACHKA1_000442"
                     /gene_synonym="yhfR"
     CDS             4607..5338
                     /gene="frlR"
                     /locus_tag="ACHKA1_000442"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO5678146.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNQRH"
     gene            complement(5498..6583)
                     /gene="yhfS"
                     /locus_tag="ACHKA1_000443"
     CDS             complement(5498..6583)
                     /gene="yhfS"
                     /locus_tag="ACHKA1_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO5678147.1"
                     /translation="MKTFPLQSLTLNEAQQKQFALVDTICRHFPGSEFLTGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAPAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RLAGIPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAHALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6595..7899)
                     /gene="yhfT"
                     /locus_tag="ACHKA1_000444"
     CDS             complement(6595..7899)
                     /gene="yhfT"
                     /locus_tag="ACHKA1_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO5678148.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDACGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWFGLFIPA"
     gene            complement(7911..8264)
                     /gene="yhfU"
                     /locus_tag="ACHKA1_000445"
     CDS             complement(7911..8264)
                     /gene="yhfU"
                     /locus_tag="ACHKA1_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO5678149.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8275..9153)
                     /gene="php"
                     /locus_tag="ACHKA1_000446"
     CDS             complement(8275..9153)
                     /gene="php"
                     /locus_tag="ACHKA1_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO5678150.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYSFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKADGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(9150..10376)
                     /gene="yhfW"
                     /locus_tag="ACHKA1_000447"
     CDS             complement(9150..10376)
                     /gene="yhfW"
                     /locus_tag="ACHKA1_000447"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO5678151.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLDEFTTHQTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLALLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVTTQIGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10377..11540)
                     /gene="yhfX"
                     /locus_tag="ACHKA1_000448"
     CDS             complement(10377..11540)
                     /gene="yhfX"
                     /locus_tag="ACHKA1_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO5678152.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNPLEA"
     gene            complement(11624..11986)
                     /gene="yhfY"
                     /locus_tag="ACHKA1_000449"
     CDS             complement(11624..11986)
                     /gene="yhfY"
                     /locus_tag="ACHKA1_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO5678153.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSTHWPAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(12003..12908)
                     /gene="yhfZ"
                     /locus_tag="ACHKA1_000450"
     CDS             complement(12003..12908)
                     /gene="yhfZ"
                     /locus_tag="ACHKA1_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO5678154.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTTLGLEATPLTDDPRFLQATEAVVLTRVDDYPIQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(13171..14175)
                     /gene="trpS"
                     /locus_tag="ACHKA1_000451"
     CDS             complement(13171..14175)
                     /gene="trpS"
                     /locus_tag="ACHKA1_000451"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO5678155.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(14168..14926)
                     /gene="gph"
                     /locus_tag="ACHKA1_000452"
     CDS             complement(14168..14926)
                     /gene="gph"
                     /locus_tag="ACHKA1_000452"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO5678156.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(14919..15596)
                     /gene="rpe"
                     /locus_tag="ACHKA1_000453"
     CDS             complement(14919..15596)
                     /gene="rpe"
                     /locus_tag="ACHKA1_000453"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO5678157.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(15614..16450)
                     /gene="dam"
                     /locus_tag="ACHKA1_000454"
     CDS             complement(15614..16450)
                     /gene="dam"
                     /locus_tag="ACHKA1_000454"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO5678158.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(16557..17843)
                     /gene="damX"
                     /locus_tag="ACHKA1_000455"
     CDS             complement(16557..17843)
                     /gene="damX"
                     /locus_tag="ACHKA1_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO5678159.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(17935..19023)
                     /gene="aroB"
                     /locus_tag="ACHKA1_000456"
     CDS             complement(17935..19023)
                     /gene="aroB"
                     /locus_tag="ACHKA1_000456"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO5678160.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(19080..19601)
                     /gene="aroK"
                     /locus_tag="ACHKA1_000457"
     CDS             complement(19080..19601)
                     /gene="aroK"
                     /locus_tag="ACHKA1_000457"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO5678161.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(20002..21240)
                     /gene="hofQ"
                     /locus_tag="ACHKA1_000458"
     CDS             complement(20002..21240)
                     /gene="hofQ"
                     /locus_tag="ACHKA1_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO5678162.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSTKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(21152..21556)
                     /gene="hofP"
                     /locus_tag="ACHKA1_000459"
     CDS             complement(21152..21556)
                     /gene="hofP"
                     /locus_tag="ACHKA1_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO5678163.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPRWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(21546..21986)
                     /gene="hofO"
                     /locus_tag="ACHKA1_000460"
     CDS             complement(21546..21986)
                     /gene="hofO"
                     /locus_tag="ACHKA1_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO5678164.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEERDAL
                     IRLRVSHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(21970..22509)
                     /gene="hofN"
                     /locus_tag="ACHKA1_000461"
     CDS             complement(21970..22509)
                     /gene="hofN"
                     /locus_tag="ACHKA1_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO5678165.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(22509..23288)
                     /gene="hofM"
                     /locus_tag="ACHKA1_000462"
     CDS             complement(22509..23288)
                     /gene="hofM"
                     /locus_tag="ACHKA1_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO5678166.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            23408..25960
                     /gene="mrcA"
                     /locus_tag="ACHKA1_000463"
     CDS             23408..25960
                     /gene="mrcA"
                     /locus_tag="ACHKA1_000463"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO5678167.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYYGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESVWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(26121..26681)
                     /gene="nudE"
                     /locus_tag="ACHKA1_000464"
     CDS             complement(26121..26681)
                     /gene="nudE"
                     /locus_tag="ACHKA1_000464"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO5678168.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            27001..29136
                     /gene="igaA"
                     /locus_tag="ACHKA1_000465"
     CDS             27001..29136
                     /gene="igaA"
                     /locus_tag="ACHKA1_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO5678169.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLIDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            29201..29869
                     /gene="yrfG"
                     /locus_tag="ACHKA1_000466"
     CDS             29201..29869
                     /gene="yrfG"
                     /locus_tag="ACHKA1_000466"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO5678170.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEFMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            29880..30281
                     /gene="hslR"
                     /locus_tag="ACHKA1_000467"
     CDS             29880..30281
                     /gene="hslR"
                     /locus_tag="ACHKA1_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO5678171.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            30306..31184
                     /gene="hslO"
                     /locus_tag="ACHKA1_000468"
     CDS             30306..31184
                     /gene="hslO"
                     /locus_tag="ACHKA1_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO5678172.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(31247..32971)
                     /gene="yhgE"
                     /locus_tag="ACHKA1_000469"
     CDS             complement(31247..32971)
                     /gene="yhgE"
                     /locus_tag="ACHKA1_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO5678173.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYCYFYQSVWHNVLILQVIFLANGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFIAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEHSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            33350..34972
                     /gene="pckA"
                     /locus_tag="ACHKA1_000470"
     CDS             33350..34972
                     /gene="pckA"
                     /locus_tag="ACHKA1_000470"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO5678174.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(35048..36400)
                     /gene="envZ"
                     /locus_tag="ACHKA1_000471"
     CDS             complement(35048..36400)
                     /gene="envZ"
                     /locus_tag="ACHKA1_000471"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO5678175.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(36397..37116)
                     /gene="ompR"
                     /locus_tag="ACHKA1_000472"
     CDS             complement(36397..37116)
                     /gene="ompR"
                     /locus_tag="ACHKA1_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO5678176.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            37344..37820
                     /gene="greB"
                     /locus_tag="ACHKA1_000473"
     CDS             37344..37820
                     /gene="greB"
                     /locus_tag="ACHKA1_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO5678177.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            37917..40238
                     /gene="yhgF"
                     /locus_tag="ACHKA1_000474"
     CDS             37917..40238
                     /gene="yhgF"
                     /locus_tag="ACHKA1_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO5678178.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            40676..40903
                     /gene="feoA"
                     /locus_tag="ACHKA1_000475"
     CDS             40676..40903
                     /gene="feoA"
                     /locus_tag="ACHKA1_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO5678179.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            40920..43241
                     /gene="feoB"
                     /locus_tag="ACHKA1_000476"
     CDS             40920..43241
                     /gene="feoB"
                     /locus_tag="ACHKA1_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO5678180.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            43241..43477
                     /gene="feoC"
                     /locus_tag="ACHKA1_000477"
     CDS             43241..43477
                     /gene="feoC"
                     /locus_tag="ACHKA1_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO5678181.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISKTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            43680..44558
                     /gene="rpnA"
                     /locus_tag="ACHKA1_000478"
     CDS             43680..44558
                     /gene="rpnA"
                     /locus_tag="ACHKA1_000478"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO5678182.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPNLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(44585..45355)
                     /gene="bioH"
                     /locus_tag="ACHKA1_000479"
     CDS             complement(44585..45355)
                     /gene="bioH"
                     /locus_tag="ACHKA1_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO5678183.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSAREEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            45393..46076
                     /gene="gntX"
                     /locus_tag="ACHKA1_000480"
     CDS             45393..46076
                     /gene="gntX"
                     /locus_tag="ACHKA1_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO5678184.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRRRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            46135..46710
                     /gene="nfuA"
                     /locus_tag="ACHKA1_000481"
     CDS             46135..46710
                     /gene="nfuA"
                     /locus_tag="ACHKA1_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO5678185.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            47071..48387
                     /gene="gntT"
                     /locus_tag="ACHKA1_000482"
     CDS             47071..48387
                     /gene="gntT"
                     /locus_tag="ACHKA1_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO5678186.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(48432..50516)
                     /gene="malQ"
                     /locus_tag="ACHKA1_000483"
     CDS             complement(48432..50516)
                     /gene="malQ"
                     /locus_tag="ACHKA1_000483"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO5678187.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQEDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(50526..52919)
                     /gene="malP"
                     /locus_tag="ACHKA1_000484"
     CDS             complement(50526..52919)
                     /gene="malP"
                     /locus_tag="ACHKA1_000484"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO5678188.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            53531..56236
                     /gene="malT"
                     /locus_tag="ACHKA1_000485"
     CDS             53531..56236
                     /gene="malT"
                     /locus_tag="ACHKA1_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO5678189.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(56279..57295)
                     /gene="rtcA"
                     /locus_tag="ACHKA1_000486"
     CDS             complement(56279..57295)
                     /gene="rtcA"
                     /locus_tag="ACHKA1_000486"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO5678190.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFKTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFPL
                     HEQYIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGQFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(57299..58525)
                     /gene="rtcB"
                     /locus_tag="ACHKA1_000487"
     CDS             complement(57299..58525)
                     /gene="rtcB"
                     /locus_tag="ACHKA1_000487"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO5678191.1"
                     /translation="MNYELLTTENASVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDGSDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            58712..60310
                     /gene="rtcR"
                     /locus_tag="ACHKA1_000488"
     CDS             58712..60310
                     /gene="rtcR"
                     /locus_tag="ACHKA1_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO5678192.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIVSVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLEVVEDEINRLRYNWQES
                     RPSALTQLLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEALQDQHSSS"
     gene            complement(60292..61050)
                     /locus_tag="ACHKA1_000489"
     CDS             complement(60292..61050)
                     /locus_tag="ACHKA1_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO5678193.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(61067..61897)
                     /gene="glpG"
                     /locus_tag="ACHKA1_000490"
     CDS             complement(61067..61897)
                     /gene="glpG"
                     /locus_tag="ACHKA1_000490"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO5678194.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(61942..62268)
                     /gene="glpE"
                     /locus_tag="ACHKA1_000491"
     CDS             complement(61942..62268)
                     /gene="glpE"
                     /locus_tag="ACHKA1_000491"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO5678195.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRNNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            62458..63963
                     /gene="glpD"
                     /locus_tag="ACHKA1_000492"
     CDS             62458..63963
                     /gene="glpD"
                     /locus_tag="ACHKA1_000492"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO5678196.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLNVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHFARTYGSNSELLLGNAGAVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLSAEQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(64178..64768)
                     /locus_tag="ACHKA1_000493"
     CDS             complement(64178..64768)
                     /locus_tag="ACHKA1_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678197.1"
                     /translation="MNKIRLYIYPLYLGFVIVPLLVWPTVVVVGITLSFLMEMLFSNS
                     FMHRRISLLELQLWLSAEYALFFCVMCCVGWQFTRRTQSELKSRLHCWLVFAPVYLWL
                     ILWNIIFYIAPAKIALLESIRNFFLLVVWLPLNFSPFWPQPWTDFIGPISAQLGFALG
                     YYCQWRSKNRCQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(64783..65547)
                     /locus_tag="ACHKA1_000494"
     CDS             complement(64783..65547)
                     /locus_tag="ACHKA1_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678198.1"
                     /translation="MQCSPAMQALIEQIYHIFRRYPVPSRFHVCCPYCFSEEQQQALR
                     GVSLRAIPFPLLHAWGTSIGPTPQNDDEIRYLLPRLFEFIAQRQFPGIYEGCCLQRIA
                     EADNQNWRTDELQIISDFAYLYMQDWVSAKEVVELEIILEMFYRGGIKIEPLLDAIMS
                     ISGYWPTASMACLLWRNSKEYVENSDFEQSDDNKTITTQINTWARNNHPLLKERARQA
                     IENPLKQPEPMTEYQVWEDDWMIDECLCAMYDASPE"
     gene            complement(65650..67158)
                     /locus_tag="ACHKA1_000495"
     CDS             complement(65650..67158)
                     /locus_tag="ACHKA1_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO5678199.1"
                     /translation="MMPNRKWILTSLIMTFFGIPILAQFLAAVVAMLGVGLTGIIEVC
                     NILITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDIWAW
                     QPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWDYLE
                     NSRTPEAYNKIVKGDADIIFVAQPSGGQKKRAKESGVTLLYTPFAREAFVFIVNADNP
                     VNSMTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIIDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(67283..69730)
                     /gene="glgP"
                     /locus_tag="ACHKA1_000496"
     CDS             complement(67283..69730)
                     /gene="glgP"
                     /locus_tag="ACHKA1_000496"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO5678200.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(69749..71182)
                     /gene="glgA"
                     /locus_tag="ACHKA1_000497"
     CDS             complement(69749..71182)
                     /gene="glgA"
                     /locus_tag="ACHKA1_000497"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO5678201.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(71182..72477)
                     /gene="glgC"
                     /locus_tag="ACHKA1_000498"
     CDS             complement(71182..72477)
                     /gene="glgC"
                     /locus_tag="ACHKA1_000498"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO5678202.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(72495..74468)
                     /gene="glgX"
                     /locus_tag="ACHKA1_000499"
     CDS             complement(72495..74468)
                     /gene="glgX"
                     /locus_tag="ACHKA1_000499"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO5678203.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDTNGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(74465..76651)
                     /gene="glgB"
                     /locus_tag="ACHKA1_000500"
     CDS             complement(74465..76651)
                     /gene="glgB"
                     /locus_tag="ACHKA1_000500"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO5678204.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(76924..78027)
                     /gene="asd"
                     /locus_tag="ACHKA1_000501"
     CDS             complement(76924..78027)
                     /gene="asd"
                     /locus_tag="ACHKA1_000501"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO5678205.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            78219..78812
                     /gene="yhgN"
                     /locus_tag="ACHKA1_000502"
     CDS             78219..78812
                     /gene="yhgN"
                     /locus_tag="ACHKA1_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO5678206.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(78858..80153)
                     /locus_tag="ACHKA1_000503"
     CDS             complement(78858..80153)
                     /locus_tag="ACHKA1_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFO5678207.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(80060..82156)
                     /locus_tag="ACHKA1_000504"
     CDS             complement(80060..82156)
                     /locus_tag="ACHKA1_000504"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HFO5678208.1"
                     /translation="MEKHAAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRGIDSAVGILLCGSEDRAIHKFFRESAFPAEAQIHEILNVLSVNDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPGAEHCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVAAGAFTQYGFKGNLPASLQGSTGRILSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(82296..83636)
                     /gene="gntU"
                     /locus_tag="ACHKA1_000505"
     CDS             complement(82296..83636)
                     /gene="gntU"
                     /locus_tag="ACHKA1_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO5678209.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(83640..84167)
                     /gene="gntK"
                     /locus_tag="ACHKA1_000506"
     CDS             complement(83640..84167)
                     /gene="gntK"
                     /locus_tag="ACHKA1_000506"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO5678210.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(84306..85301)
                     /gene="gntR"
                     /locus_tag="ACHKA1_000507"
     CDS             complement(84306..85301)
                     /gene="gntR"
                     /locus_tag="ACHKA1_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO5678211.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTAAIIARGHRHIAYLGARLDERTIIKQKGYEQAMRDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(85525..86220)
                     /gene="yhhW"
                     /locus_tag="ACHKA1_000508"
     CDS             complement(85525..86220)
                     /gene="yhhW"
                     /locus_tag="ACHKA1_000508"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO5678212.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(86343..87380)
                     /gene="yhhX"
                     /locus_tag="ACHKA1_000509"
     CDS             complement(86343..87380)
                     /gene="yhhX"
                     /locus_tag="ACHKA1_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO5678213.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            87715..88203
                     /gene="yhhY"
                     /locus_tag="ACHKA1_000510"
     CDS             87715..88203
                     /gene="yhhY"
                     /locus_tag="ACHKA1_000510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO5678214.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGTVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT        21358 a        24134 c        22078 g        20740 t
ORIGIN      
        1 aatgaacaaa tttattaaag ttgcactggt aggtgcggta ctggctacgt taactgcatg
       61 tactggtcat attgaaaacc gtgataagaa ctgctcttac gactacctgc tgcacccggc
      121 aatttctatt tctaaaatca ttggcggttg cggtcctact gcacagtaag tctctgacag
      181 aaatcggcta acaccggttt aattgcccga cgccctctgg attttccgag ggcgtatttg
      241 tttctatatc cttcttgctc cccacccgta aaaatatttt cactttaatt tcaattggat
      301 aaccacattc atcagccaca gcgcgatcct gtgcgaaatt atgtgatctt cctccacatt
      361 acataacatc atatgttgtt atattcatca tgcattgtca tgttaccttt taaatgactg
      421 caaactctcc cctacaacgt gttggacaag aaaaaggtat cgctatggga agccaggaac
      481 tccaacgcaa gctcggattt tgggccgttc ttgcaatcgc cgtcgggaca accgtcggct
      541 ccggtatttt tgtatctgtg ggtgaagtgg caaaagcggc gggcacgccg tggctgacgg
      601 tgctcgcatt tgtcatcggc gggttaattg tgatcccgca aatgtgcgtc tatgcggaac
      661 tatccaccgc ttatccggaa aatggcgcag attatgttta tctgaaaaat gccggaagcc
      721 gaccgctggc tttcctctcc ggctgggcca gcttctgggc caacgatgcg ccgtcattgt
      781 cgattatggc gctggcgata gtcagcaatc ttggcttttt aacgcctatc gatccgttgc
      841 tcggtaaatt tatcgccgcc ggattaatta tcgcctttat gctgctgcac ctgcgctccg
      901 ttgaaggcgg cgcagcattt cagacgctaa ttaccatcgc caaaattatc ccgttcacta
      961 tcgtcattgg ccttgggatc ttctggttta aagcggagaa ttttgccgcc cctaccacca
     1021 ctgcgattgg cgcaacgggc agctttatgg cgctactggc ggggatctcc gccaccagtt
     1081 ggtcgtacac cggcatggcc tctatctgtt atatgaccgg cgaaattaaa aaccccggta
     1141 aaactatgcc acgagcgcta attggttcct gtctgctggt tctggtgctc tacaccctgc
     1201 tggcgttggt gatttccggg ctgatgccct tcgacaaact cgccaactct gaaacgccga
     1261 tttccgacgc actgacctgg atcccggcac tcggcagcac cgccgggatc ttcgttgccg
     1321 tcacggcgat gatcgtcatt ctcggctcgc tttccagttg cgtgatgtac cagccgcggc
     1381 tggaatatgc gatggcaaaa gacaatctgt tcttcaaatg cttcggccat gtgcatccga
     1441 aatacaacac gccggatgtc tccatcatcc tgcaaggggc gctggggatc ttcttcatct
     1501 tcgtttcaga tctcaccagc ctgctgggct atttcaccct ggtaatgtgt ttcaaaaata
     1561 ccctcacctt cggctccatc atctggtgtc gtaaacgcga cgattacaaa ccgctgtggc
     1621 gtactccggc tttcgggctg atgaccaccc tcgccattgc atcaagcctc attctggttg
     1681 cctcaacctt tgtctgggca ccgattcccg gccttatctg cgccgtcatc gttattgcca
     1741 ctggtctgcc tgcttacgcc ttctgggcga agcgtagccg ccagctcaac gctttgtcgt
     1801 aatgttatct ggagaaaata aaatgttgga tattgataaa agcaccgtgg actttctggt
     1861 caccgaaaat atggttcagg aagtggaaaa agttctcagc catgacgttc cgctggtgca
     1921 caccatcgtg gaagagatgg tgaagcgcga catcgatcgt atttatttcg ttgcctgcgg
     1981 atcgccactc aacgccgcgc aaacggcgaa acatctggca gatcgctttt ccgatcttca
     2041 ggtttacgcc atttccggct gggagttctg cgataacacc ccgtatcgcc tcgacgatcg
     2101 ttgcgcagta attggcgttt ctgactacgg taaaaccgaa gaggtaatca aagcgctgga
     2161 gctgggccgg gcctgcggcg cactcactgc ggcgttcacc aaacgcgcgg acagcccgat
     2221 tacctcggcg gcggaattta gcattgatta tcaggccgac tgtatctggg aaattcatct
     2281 gctgctctgc tacagcgtgg tgctggagat gatcacccgc ctcgcgccgc acgcggaaat
     2341 cggcaagatc aaaaacgatc tcaagcagct gccaaatgcg ctcggtcatc tggtacgcac
     2401 ctgggaagag aaaggtcgcc agctcggtga acaggccagt cagtggccga tgatttacac
     2461 ggttgccgca ggcccgctgc gcccgctggg ttacaaagaa ggcatcgtga cgctgatgga
     2521 atttacctgg acgcacggct gtgtgattga gagcggtgag ttccgtcacg gcccgctgga
     2581 gattgtcgaa ccgggcgttc cgttcctgtt tctgctcggc aatgacgaaa gtcgtcacac
     2641 caccgaacgc gccattaact ttgttaaaca gcgtactgac aacgtgatcg tcatcgatta
     2701 cgccgaaatt tcgcaagggc tgcacccgtg gctggcaccg ttcctgatgt tcgtgccaat
     2761 ggagtggctc tgctactacc tgtctattta caaagatcac aacccggatg aacgccgcta
     2821 ttacggtggt ctggtggaat attaatccct ctcccggccc ggcaacgggc cggatttacg
     2881 caaggagtta cccgatgaaa acaggtatgt ttacctgcgg ccaccagcgg ctgcctattg
     2941 aacacgcatt ccgtgatgca agcgagctgg gttacgacgg catcgaaatt tggggcggtc
     3001 gcccgcacgc gttcgcgccg gacttaaaag cgggcggcat caaacagatc aaggcgctgg
     3061 cgcagacgta tcagatgccg attatcggtt atacgccaga aaccaacggc tatccgtata
     3121 acatgatgct gggcgatgaa catatgcgtc gcgaaagcct ggacatgatc aagctggcga
     3181 tggatatggc aaaagagatg aacgcgggtt atacgctgat ttctgcggcc cacgcgggct
     3241 atctcacgcc gcctaacgtt atctgggggc ggctggcaga gaacttaagc gaactgtgtg
     3301 agtacgcgga aaacatcggc atggatctga tcctcgaacc gttaacgccg tatgaatcga
     3361 acgtcgtgtg taatgctaat gatgtgcttc atgcgctggc gctggtgcct tcgccgcgcc
     3421 tgttcagcat ggtcgacatt tgcgcgccgt atgtccaggc ggaaccggtg atgagttatt
     3481 tcgacaaact gggcgataaa ttacgtcatc tgcatattgt cgacagcgac ggggccagcg
     3541 acacgcatta cattcctgga gaaggcaaaa tgccgctgcg ggaactgatg cgcgatatta
     3601 ttgatcgggg ctatgagggg tactgcacag tggaactggt aacgatgtat atgaacgagc
     3661 ccagacttta cgcccgccag gcgctggaac gctttcgcgc gctgctgccg gaggatgagt
     3721 gatgaaaacc ctggcgacaa tcggcgataa ctgcgtcgat atctacccgc aactgaataa
     3781 agcgttttct ggcggtaatg cggtgaacgt ggcggtgtac tgcactcgct acggtataaa
     3841 gccgggatgc attacctggg tgggtgacga tgactacggc acaaagctga agcaggatct
     3901 cgcccgcatg ggcgtcgata tcagccatgt ccatacgaaa cacggcgtaa ccgcacaaac
     3961 tcaggtggaa ctgcacgaca acgatcgcgt ttttggcgac tacaccgaag gcgtgatggc
     4021 cgactttgcc ctgagcgaag aagattacgc ctggctggcg cagtatgaca ttgtgcacgc
     4081 ggcaatctgg ggacatgcgg aagacgcatt cccacagctg cacgctgcgg gcaaacttac
     4141 cgctttcgac ttctccgaca agtgggacag cccgctctgg cagacactgg tgccgcatct
     4201 cgattttgcc tttgcctctg caccgcaaga agacgaagcg ctgcgtctga agatgaaagc
     4261 gattgttgcc cgtggcgcag gaacagtgat tgtcacgctg ggcgaaaacg gcagcattgc
     4321 ctgggatggc gcgcagttct ggcgtcaggc tcctgaactg gtgacggtta tcgacaccat
     4381 gggtgccgga gattcgttca ttgccggatt cctttgcggc tggtctgcgg ggatgacatt
     4441 accgcaggcg atggcgcagg gaacggcgtg cgcggcgaaa accattcagt accacggtgc
     4501 ctggtaggta taacgttggc gtgagcatct tcacgccaac gtgctgttac ttgccggaaa
     4561 acgaccctat aatccgagta attcattctt tatttcaggg tcgattatgt cagctacgga
     4621 ccgctactct catcaactcc tctacgctac cgtccgccag cggctgctgg atgatatcgc
     4681 gcagggggtt taccaggccg ggcaacagat ccctaccgaa aacgagcttt gtacacaata
     4741 taacgtcagc cgcattacca ttcgcaaagc catcagcgac ttagtggcag acggcgtact
     4801 gatccgctgg cagggcaaag gcacctttgt acaaagccag aaggtcgaaa acgccctgct
     4861 tactgtcagt ggttttaccg attttggcgt ctcacaaggc aaggcgacga aagagaaagt
     4921 gatcgagcag gaacgggtca gcgccgcgcc gttttgcgaa aagctgaaca tccctggcaa
     4981 cagcgaagtg ttccatcttt gccgggtgat gtatctcgat aaagagccgc tgtttattga
     5041 tagttcctgg atcccgctgt cgcgttatcc tgactttgat gagatttacg tcgaaggaag
     5101 ctccacctat cagttatttc aggagcgttt tgacacgcga gtggtcagcg acaaaaagac
     5161 catcgatatc tttgccgcca cccgcccgca ggcaaaatgg ctgaaatgcg aactgggcga
     5221 accgttgttt cgcatcagca aaatcgcctt tgatcagaat gacaaaccgg tgcacgtctc
     5281 cgaactcttc tgccgcgcca accgcatcac cttaacgatt gataaccaac gacattaacc
     5341 gtaggccgga taagatgcgc cagcatcgca tccggcgatg ccggcgcact gaattttaca
     5401 tcccgcacgt tcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcagtgc
     5461 ggaatttagc ttgattgccg aacggtgcta tccagcatta aatactggca atactctcac
     5521 gcaaaatccg cagcaccgtc tcttcaccgc tgcgattcgg gttaatgcga atcgcacaat
     5581 gttcgagttg cggattcgcc tggcggaacg ttccggaaag gcgataaaag agcggcggga
     5641 tttcatattt cgactctgca ccgaccgggt aaggcaacgc gccacgcttt tgcgcttctt
     5701 ccagcaccct ggcggcaatc ggctgatgaa actcgacaat caacaccttc gactgcgcat
     5761 tggcaatgac ggcgcttttc acttccggca ctgcaccacc gttgagcaaa gccagcaacc
     5821 gttcagatac ccctgcctgc accgcgtgca tcactggcgc gaacaccagc ccacgcaaca
     5881 cttccaacgc atgagcgccc tggatctggc tgccgccgga gtaaagcgtg gcgcgaatcc
     5941 gactaataac atcggcatcg ccaaccactg caccaacgcc ctccggccca aacagcttga
     6001 agcaggagaa tgtcgagaca ttcgcgccgc attcacagcc gattcgcgcc accttcatca
     6061 ccgcatagtt gtcatcggtt aacgctggaa tgccagccag gcgcaacgtt gccagcacat
     6121 ctgccagcac atagctgtcc tgcggctgct ggcgcgtatg ctgcaccagc gcggcatcag
     6181 gttgttgctc gtcgacgacc tgcttcagtg ccgacaggtc attgaaatca gcagtaataa
     6241 gcgtcagccc catctgctca ataataaccc gtgtcgttgg gtaaacaggc gcgtcatgca
     6301 ccagaagacg ctgccccggt ttgagcaaag ccgccagcgc cgcacgaatc gcgccagtac
     6361 ccgccccctg taccagcgcc gccgctggtg catgaaacgc atcagccagc acctgctcca
     6421 cccgctgggt aatgcgcggt tgattcaacc ctggagttaa ccctaaatca ccgccagtaa
     6481 gaaactcgct gccggggaaa tggcggcaga tggtgtccac caacgcaaac tgcttttgct
     6541 gcgcttcatt aagcgtcagg ctttgcagag gaaacgtctt cataggtgca ttccttaagc
     6601 cggaataaac aggccaaacc agtaaagaac attcagcagg atgccggtga tcattactgc
     6661 cacgaccggt gccgccattt tctgtaccgg acggcccagg gattcgttga ggaagtaaat
     6721 ggcaaccgcg atagagaatc cggtataacc cgccatctta attgctgcga aaatcgaacc
     6781 gaccagcagc gccacttcca tcagcatatt catggcgtta cggatgttat ccgacgcatt
     6841 acgcaccgac gggtagcgtc ccagccattt gccgatagaa cgaagcagca agacttccgc
     6901 cgaaataacc actgcgccta atactgccgc aaccatcgga ttcggtgaga gatagcccac
     6961 cgcataaaca aaggtaaagc ccgcaaccgc atacacgcca gtggctaacg cggtggtggc
     7021 aatcaacggc acaaaaccca gcccgcgcat aaattccgcc agtgccgcct gattaatcag
     7081 cgtttgtgat tgttccggcg ttacgcctgc ggagtacgct ttctccagag tgaagatcga
     7141 cacttcactg ccggcgaaaa tcttcatgct ggcaacggcg gcaatcagcg cccctacgat
     7201 ggcgatatac ggtaagtttt tgataatccg cgacgtgcgc tcttcaaaca ccgaaagccc
     7261 gcaggcatcg atgtcatttt catcacgatg acgcaggtcg tgagttatcg cgatccccag
     7321 cagcatcacc atgccaataa agatttcgat ggattcaggg ttaagatgtg ggaaatagcg
     7381 cacgactacc acacgggtca tcagaacaac cacggcggcg accagacttt gcttccagcc
     7441 aaactggtag aaaatcgcca ccagcgggaa cagtgcaaaa gcagaaacca ccggcgagct
     7501 taattccccg aggctaccta atacatccac cggcagcgcg gtcagcagct ggtttactgg
     7561 caacaggcaa gtaaggatca acacgcccca gatagcgcca agaccaaacg ccatcaggct
     7621 gtttatcgcc agtacgccga ggatatcggt aggaagaaag agtaaccatg cgtttagcag
     7681 cccggttttc agggtaaaag agatccccac cgaggccaca aaaccaatgc tcagaccaaa
     7741 agcgatactc cccgcctcgc gacggttcat atagccttca atcagttgcg gcaggatcgg
     7801 gcggatgccg tcatgaaaaa cagccgccga gcgatgcgcc agaagcgatg tcatacccgt
     7861 caggcacgcc accacgataa tctgaatata cagatccata gtcgtgcctt ttatttcaga
     7921 tggttaatca gcatcggaat cgcgtgttcg acgtgctcaa cggaaaggcc aaacgccact
     7981 tttccttcag cgatcatttt ggcgatatgc tcgtctttcg ctttaatgcc tggtttggca
     8041 atggtgcaac ttttgttata gccaatcacc gcgatggcaa tcgacaatgc cgcgcccgca
     8101 ccggtattac acgcgccgat gtaataatcc agttgcccgg atttcacctt cattgccgct
     8161 tccatgtcgt tgtgaatgaa aacttcaaaa cagccaggag ccgtcgcttc aatagttttt
     8221 ttaatctgct cacgctgtaa gcctgcaacg ccaatctttt tcatgagtct gtccttattg
     8281 gaaaaattga gagggatttt cacgtaacat cacatccaca tcggcctgac tgaatcctga
     8341 ctggcgcaat tgcggaataa aggtggttag taaatagtca tagccataac caccgtcggc
     8401 ttttaaatgg gagcggcgcg taatatccat cgacagcatg acgcggttca gcaacccacg
     8461 gtcgcgtagc gcatgaagca tcgcaatacg cttttcgtcc gggtagtaac tgttcttacc
     8521 aatggtgtcg aactgcacat acgcgccgag atcgatcatc ttcaaaatgt tgtcgaggtt
     8581 gtctttcaga tcgcagtgac caacggtgac gcgcgaaaga tcaactccgt gggcttgcaa
     8641 caaagccagt tgctccagcc ccatcgtgct gaacgacgta tgcgtggaga tcgggcgtcc
     8701 ggtctggtta tgtgccagcg cggcggcaat aaataccttc tcttccagcg gcgtaatctt
     8761 cccttcgctg gagccgatct ccgcgatgat cccggctttc agttccgtgc catcgatacc
     8821 ctgctcaatt tcatcgacca tctcctgcgc cagttcctgc acgctgcggg tcgccacatg
     8881 ttccgggaaa aacgcgtcct ggtaataacc ggtacaggcc accacgttga tccccgtctc
     8941 gcgcattaca tcgagcataa attgcgcatt gcgccccatg taacggttgg tcatctcaat
     9001 cacattacgc acgccccggg ccatcaggtc gttcatctcc tggcaaatga acgaatactg
     9061 atcaaggcgg cagtccacgt tgtttttaaa gccggagaga tcaatatgca gatgctcatg
     9121 ggcgagggtg taacccgtcg gatcaaaact catagggtgt ctcctgcaaa ccggagggag
     9181 gaaagaaaag agcgaccatt ttgtggcggt ggcgcacgga aaaattcaca cacggtagcc
     9241 cccacatcag aaagcgtggt gcgcacaccg atctgcgtag tgaccagccc ttgctgataa
     9301 accagcactg gcaccacttc acgggtatgg tggctgtgac caatggtcgg atcgttaccg
     9361 tgatcagcca tgacaaccag gcaatcatct ggctgcatcg cctcaaccag gagagcaaga
     9421 ttacggtcaa caacctgcaa acgttcggca taacgtgcga cgtcttccgc gtgaccagcg
     9481 aggtcggttt cctgaatgtt ggtgcaaata aacgctgtct gatgggtggt aaattcgtcg
     9541 agagtgatat ccataatccg ctggctatcc accagatttt gccagctcac gccataagga
     9601 ttgttgacga tatctgccac cttacccacc agcacggttg gcacgcctgc ttcatacagt
     9661 ttttgtggca cctgcacttt ttcatcgacg ccatagccca tatgcacgac ctggaaaccg
     9721 ttgtcataag cgccagaacg cggcgcatta ataccaataa agcgcccttc tttgctttct
     9781 gcggcatcga gaatacgctg actgtcggtt aacaggccgc caaatgtaat gacccgaccg
     9841 acctgtacct ggtcacgcac gatacgacca attttgattg cgtcgtcaaa agagatcaca
     9901 gagagattgg cggtaatgtt atagacttgg cctaaatccg cctcgagatt atcgccaatc
     9961 gcaaccgcct gattgaccca cagaaattgc agttcatcgc cacggcgctc cacctgccaa
    10021 ccggcggata ctaatgcctg ctcaacacgg tcaatcacat cgcgaaaggg catccgcagc
    10081 ggcggtaacg ggcgcgtgcc taaaatttcc tgatgcccca taaaggtatc gccaccttca
    10141 tgttgcagct ctgccacgcc ccaggttgcg gagtctgacg gctgcatatc gcctggcgca
    10201 taacccaatg cgttgattag ccccagcttc tccagcgttg gtagctgcaa atgcggcaac
    10261 tggctcagga tgtgaccaca ggtattcgcc ccggcatcct gcggacgcac cagcgtgaca
    10321 tctttcattg cccctacgcc aaagctatca atcactaaca ccacaaatcg cgccatttac
    10381 gcctccagag ggttgccgag actgtcataa cgaccaacga ttttcggttc gccacggtga
    10441 ataccggaca ccagcaccac atcgctacgg gtgacaaaaa tctgcgtgcg gaaacagagc
    10501 accactgcac tgcttaccgg aaactcgcct gccagcgaca gggtgtagtc gatactgctg
    10561 tcatccacag ttttgagatt ggtttcagta atcttttgat tttctggcgt aaacaccagc
    10621 gcatgttgcg catgaccacg acggtagtaa ccgccgccgt agcagtagct gtcgccacgg
    10681 aaatgatggg agatttcact taaccagagc atcgcgatac gttcaggctg atcgccctgc
    10741 tggtttgccg gaatagtacc ggtcagcgca tgaccgggtt cggcatgagt cgcaccgtat
    10801 tccgccagca atggcagcga agtgcagctg gtcgctgaag gcgcgttcag ttgctcaatc
    10861 gcaataccag atttcgccag ttgatcccgt gcctgtatca gcgtgtgaag attcggtgtc
    10921 ggcaaaactt tcccggcagc ctcatcccaa agcaggcaag ggaaatgggt tagtccggct
    10981 aaatgcagcc ccggcagttt cttaatttca gcaaccacct cgtgcagcga atgctgtaca
    11041 aaaccgctct cctgccccgg ataaagaaaa tcatcgtcgc tataaacttt gagcagcaca
    11101 gactggattc gcccggcctt caccgccgcc gcagaaactt cccgcgcttt gtcgagagta
    11161 aacacggtga tgacgtcggt gccctgttca acggcgtcag caacctgatg acaagggatt
    11221 tgtaccagat gcccctgatg cgccaccggc aaaccagcgc ggcgcatgac tcgcgcctct
    11281 ttgtaatcca ccgccacaat gccgctgtag cccaacgcca gcagtttttc cgccagccat
    11341 gggttacgac cgaattgttt ggtcatcaga tacagttcaa tgccgtaaag ccgcgccgtc
    11401 tcaatcagcc gtttaccgtt ttccagtacc tgatccacgt cgatcaccca gctgtccggg
    11461 gcgatcttgc cctgctgcca caaacttagt gcggcggaaa tcagcgccgg gttctggcgt
    11521 ttcaatgctt ctacaaacat cttgttgcct cgtttctgtt ccttgaatta aatattcatt
    11581 tttttgaata tttagattgt acgacgtgga tttgtcggga gcttcaaacc tcttcgttag
    11641 cagccatcca taatccataa aggttggcca gcaaatagcc ttcttcacac gggttaactt
    11701 ccagtgcgaa ttccttcaac aacacctgat gcaattgcac aacggcaggc cagtgagtgg
    11761 attgcgccag ctctgccagc aattcgtcat ccagcggctc tatttcttca ccacggcgac
    11821 tgcgcatcag tgcactcgcc atatgtgtca tcgccatcgt tccttgctca ctgcgcaccg
    11881 gtagatggca ctctgtttcc agtacgttga cgacctgcat catgcctttg cagatgtcct
    11941 tatcaataac gcctgcatcg caaagcaggt tgagtctggt ttccatatca gatgcctttc
    12001 gtttaataac tgggttcctg ttccccactc accacccgtt gttgatgggc aagcagggcg
    12061 tgtttatcaa caactgcacg cagcagttgt tgtattgggt aatcatcgac tcgcgtcagg
    12121 accactgctt cggtggcctg taaaaatcgc ggatcgtctg tgagcggcgt cgcctctaat
    12181 cccagcgtgg tcagttcgtt ttccgccacc acgttccaga taaccgcatc aacatcgcct
    12241 ttaacaatgc gttgtaaact ctcgtgataa gagagatcga ctcgttccac atcactaccg
    12301 ccaaaaaaga catcagtcat gattttctga tccgccgaac ggttatccag ccccacgcgc
    12361 ttcacgtttg cggactcgcc tttacggcaa atcaactggt gctcgccaac gtaggtgtgc
    12421 ggccccaact ccagcgcgag gcataaccct ttttgcgtga gataactttc cgccgccagt
    12481 cgggaaacca ccgccatgtc atacacgccg ttaagtaaac actccacgcg aatatccgcg
    12541 ccgcgcatat gcgcatagta aaaaggaatg ccatcaaact gggctttcaa tccgctcgct
    12601 aaaccttcgt acaagcgggt atagggcaaa ggcatcgcac ataccacgtt gttgatatcc
    12661 acatgactca gcaatgcttt gttatccatc tcgaccagat aactaccatt gcgcccacgg
    12721 cgttcaatcc gtatcgctcc gcttgattcc agcgttttca acgcggcctg cgtcagacca
    12781 acggatgaac ggcattcagt ggccagttca tctatagttt tcagtcgatt accgcacttt
    12841 tcacccaaca aataacgggc cagcgtcgtg ataacgacgc cttctttttt gataaacgtt
    12901 cgacgcataa tatattttca ataaaatgaa tgtttatatc ttcattaaaa tgaagataag
    12961 tggcaacggt gagatgtgga aacggcgagg cgcttcacat tttttcggat tattgcaaat
    13021 gaggataaaa aaaccgggtt tccccggttt cagagtgatg acgaagtgat ttattcgaca
    13081 atgcgagcaa agggtaattc ccactggcgc ttgcctgctc ccctcaccct ctccccaaag
    13141 gggcgagggg accgtccgag cgttttgata ttacggcttc gccacaaaac caatcgcttc
    13201 gtacaccgct tttagcgtac gggaagcgtg cgcgctggct ttttctgcgc cgtctttcat
    13261 cacctgttgc aggaaggctt catcgttgcg gaaacggtgg taacgttcct gcaattcagt
    13321 cagcataccg gaaacggcat cagccacttc gcctttcaga tgaccataca tcttgccttc
    13381 gaactgtttt tccagttccg ggatgctctg gcccgttacc gcagaaagga tatccagcag
    13441 gttggaaacg cccgctttgt tctgcacatc gtagcgaact accggcggct cgtcggaatc
    13501 agtgaccgcg cgtttgattt tcttcactac cgatttcgga tcttccagca ggccaataac
    13561 gttattgcgg ttatcgtcag acttggacat cttcttggtc ggctccagca gcgacattac
    13621 gcgcgcgccg gatttcggaa taaacggctc cggcacctta aagatctcgc cgtacagcgc
    13681 gttgaaacgc tgagcgatat cgcggctcag ttccaggtgc tgtttctggt cttcacccac
    13741 cggtaccaga ttagtttgat acagcaggat gtccgcagcc atcagcaccg gatagtcaaa
    13801 cagaccagcg ttgatgttct cggcataacg cgcagattta tctttaaact gcgtcatgcg
    13861 gctcagttcg ccgaagtagg tatagcagtt cagtgcccaa cctaactgtg catgttccgg
    13921 cacgtgggac tgaacaaaaa tggtgctttt ctcaggatcg ataccacaag ccagatacaa
    13981 ggccagcgta tccagcgtcg ctttacgcag cttctgtgca tcctggcgca cggtgatcgc
    14041 gtgttggtca acgatacagt aaatgcaatg gtagtcatcc tgcatgttta cccactgacg
    14101 cagcgcaccc atgtagttac caatggtcaa ttcacctgag ggctgtgcgc cactaaaaac
    14161 gatgggctta gtcatttttc gattcctgat tttcgctatg cggaagccct aatgcgggca
    14221 gaaggtcatt tatagactga taaattacat caggctggct gagatcgata gcctcgccgt
    14281 agttatatcc gtaggttaag ccaactgatg ggcaacctgc cgcttttgct gcctgaatat
    14341 cattgcgtga gtcgccaaca aacagcatct gttgtggagc aattcccatc cgctcagcca
    14401 ccagtaacag cgggtccgga tgcggttttt tgttttgcac atcatcacca ccaatcacca
    14461 cgctgaagta tttagcgata tctaaggctt cgagcagcgg cgcgacgaac ggcgtcggtt
    14521 tgttggtgac caggcctagc ggcaggcctt tagcctgcaa cgcgcccaac gtatcggcaa
    14581 cgtgcgggaa caaaaacgtc ccctcttcgg caacctcgcc atagtagcga tcgaacagtt
    14641 tacgcagaat acgtacctgt tcttctgccg gaatgtcgtc atcaacgggc ggtttaccca
    14701 ttgttttacg ctgagtcgca cgttcctgac gcgcccaggt caatgcgcgc tccatcagaa
    14761 catctgcgcc gttaccaatc caggtaataa cgcgttcttc acctgcgacg ggcaactcca
    14821 gcgcatacag cgccatatct accgcagcag caagaccagg agcactgtcg accagcgtac
    14881 catcaagatc aaaagcgacg ccgcgaatat cttcaaactt attcatgact tacctttgcc
    14941 agttcactgc gcatttcatc aatgactttt ttatagtctg gctggtcgaa gattgccgaa
    15001 ccggcgacga acatatccgc gcccgccgca gcgatttcgc caatgttgtt caccttcacg
    15061 ccaccgtcca cttccagacg aatgtcaaag ccagactcgt cgatacgacg acgtacttcg
    15121 cgcagtttat ccagtgtttg cggaatgaaa gactgaccgc cgaaaccagg gttgacggac
    15181 atcagcagga tcacatccag cttatccatc acgtaatcca gatagctcag aggtgtcgcc
    15241 gggttaaata ccagacccgc tttacagcca ttttctttaa tcagttgcag cgtgcggtca
    15301 acatgctcgg aggcttctgg atgaaaggta atgatgctgg caccagcggc agcgaaatca
    15361 ggcacaatgc gatcgacggg tttcaccatc agatgtacgt cgataggggc ggtaatgcca
    15421 tagttacgca aggatttcag caccattggc ccaatcgtca gattgggaac atagtggtta
    15481 tccatgacgt caaaatgcac gacatcagcg ccagctgcca gggcttttgc ggtatcttca
    15541 cccaggcggg caaaatcagc cgacagaatt gagggggcaa tcaaatactg tttcatccgc
    15601 ttctccttga gaattatttt ttcgcgggtg aaacgactcc tggtttgtac aaagccagca
    15661 gttcgtccac ctttttacgt gtgccgccgt tgctgcttat actgcgtcga actttgacga
    15721 catgcaattt tgcgcgctga taccactcac gcgttaacat cgtatcgtga ttggagatca
    15781 gcacaggaat atggcgatca accagacctt cggcgatctc cgccagatgc gcttgttgtt
    15841 caagcgtaaa actgtttgtg tgatacgccg taaagttggc ggtcgcagac agcggcgcat
    15901 aaggcggatc gcaatagacg acggatgcat catctgcacg cgccatgcta tcggcgtaag
    15961 actcacaata gaaaaaggca ttctgcgctt tttcagcgaa gtgatacaac tctgcttccg
    16021 ggaaataggg ttttttgtag cggccaaacg gcacattaaa ctcaccgcgc agattgtaac
    16081 gacacaggcc gttgtaaccg tagcggttca aatataaaaa cagtaccgcc cgacggaacg
    16141 gatcctggct tttgttgaac tcttcgcgga actgatagta aacctcggcg caatttgttt
    16201 cgggaacaaa cagctcgcgt gcggcctgta cgtactcatc agtacgcatc ttcacaatgt
    16261 tatagagact gatcaggtcg ctattgatat cggcaaggat ataacgagaa aagtcggtgt
    16321 tgagaaacac cgacccggca cctacaaaag gctcaaccag acattcgccc ttgggcaaat
    16381 gccgtttaat atcatcaagc aggggatact tgccccctgc ccacttcaaa aaagcgcgat
    16441 tcttcttcat gctgactaac taattacacc ttctccggct gtggagaaag ctccgacagc
    16501 atccagcgct tcaggcaact cccgcaacga taaaccattg cgggagataa ccttgattac
    16561 ttcagatcgg cctgtacctg acgcagcggt ttcgcccacg ggtttttggc ctggacatct
    16621 gctggcaatg tagataccgc ttttttcgcc tcttctttcg aagcgtacac gccagaaacc
    16681 aggacatacc acggctgacc attacgcgtc gtttcataga caacgtagtt tttcagattc
    16741 tctttcttcg cccaaccgtt caggttgtcg tagttagagg aactgctcag ctgcagagtg
    16801 taatggctgg acggtgccga tttcaacgaa ccaacattac ctgcggtctt ccctccagcg
    16861 gctggtgttg ccgtggtttg cgccggggat gccgtctgta ctggagccgt agtcgccgtc
    16921 tcttttggtg ctggcgtaga agtcgcagcc ggtgccttcg tgctcgccac aggagcagct
    16981 ggttcagtac gcttcggcgt ctgtgctacc ggcttcggtt ccgttttcac ggttgcttgc
    17041 ggttttttcg gttcaatcac cgcctgctga cgagctggac gcgtagtggc cggacgttca
    17101 gcagtttgcg ttttcgccgt gtcacgcgat gcattgccat tgcgtactgg cgcgacagtt
    17161 gctggttcag tcggcaatgt agaattgacc gccacattgt tcaactgttg ctgattttgt
    17221 ggctgggtca gcgcattgtt caggtcaccc tgcacttcaa cacgttgttg accatccgtt
    17281 gccaccgggg tttgcccttg agtcggcgta gaagagatcg gtggcagaga aacatcctgc
    17341 tgagtatttt ccgcagacgt ggttcccggc gctggctgca cgccattcgc ctgatcggtc
    17401 gcattgccag caagatcaat actcttctcg ccagacgcgg tttgatcgct ggaagtggtc
    17461 gagggggctt ttagcgcaga accgataccg atgatcaaca gcagtagaac cagaatgccg
    17521 acgcccatca tcatatactg acgagaagcg ggtttactgg ctgctttttt gcgcttacgc
    17581 ggacgacgct ctacgcgctc ttcatccacg gtttcatctt cggattcgtc aatttcttct
    17641 tcgatttccg gttcctcatt gcgctctttt tgcgcacgag tcggacggcg atcgtcagtg
    17701 tcatcaagtt caatatcatc aaaattgatc tgcggttcgc cacgttcagt acgctcagaa
    17761 cgttcagaag attgacgaga acgaccagta cgacgatcgc tgggatcggg tttcagctcg
    17821 tcttctggtt tgaattcatc catttaacac cccactaaaa ggttaatgct taccacgttg
    17881 caattaacct gaagctaata gaccgcttga taagcggcct gacctttctt gttgttacgc
    17941 tgattgacaa tcggcaatgg cgttaagaac aagctcgtgc gaaacgccgc tgcgaacttc
    18001 actcttacca attgccaacg gaagaattaa gcgcatctct cccgcaagga ctttcttgtc
    18061 acgcagcata tgcggtaaat acgcctgcgc ggacatttcg cgcggcccat tgaccggtaa
    18121 cccagcccgc ttgagcaggg ttataatacg ctgcgtttcg gcagaactaa actgcccgag
    18181 acgttccgac gtccgcgccg ccatcaccat acccgcagcg accgcttcac catgtaacca
    18241 attgccatac cccatttcag cttcaatggc atgaccaaag gtgtgtccca gattcagtaa
    18301 agcacgtaac ccggtttcgc gctcgtcggc ggcgacaact tctgccttca gttcacaaca
    18361 acggcgaata cagtacgcca ttgccggacc gtccagacgc aacaacgcat ccagattctc
    18421 ttccagccag ttaaaaaacg caccgtcaag aataatgccg tatttgatga cttctgccag
    18481 ccccgacgct aactcacgcg ggggaagcgt tttcagacag tcgagatcca ccaccactga
    18541 agcaggctgg tagaacgcgc caatcatgtt tttaccgagg ggatggttga ccgcagtttt
    18601 gccgccaacg gaggaatcga cctgcgacag taacgtcgtc gggacttgaa tgaaacgaac
    18661 accgcgctga taactcgccg ccgcgaagcc ggtcagatcg cccactacgc cgccgccaag
    18721 cgccaccagc gtagtatcgc gaccatgcgg tttttgtaac aacgccgtaa agacggtatc
    18781 gagtacagcc aggcttttat actgctcgcc gtcagggagg ataacgctat cgacgttaac
    18841 acccgcctgt tcaagtacgc cgcggacctt atcgagatac agaggagcca gggtttcgtt
    18901 ggtgaccaac atgacctgct cgcccgattt cagcggtaag aatgaagctg gttcattaaa
    18961 caaaccagat gcgatggtaa ttgggtaact acgttccccg agagtaacga caatcctctc
    19021 cataacgcga catccacctt aattactgta cccgcagacg agtgtatata aagccagaat
    19081 tagttgcttt ccagcatgtg aataatctgg tttgcaacca ctttagcgct ttgatcatca
    19141 gtacgaatgg tcacgtcggc aatctcttca tacagcggat tgcgttcatt ggccaacgct
    19201 tccagaactt cacgtggcgg tgtttcaacg tgcagcaacg ggcgtttttt atcacgctgc
    19261 gtgcgtgcaa gttgcttttc gatggtcgtt tcaagataaa cgacaacgcc acgagcggaa
    19321 agacggttac gcgtttcacg ggatttcaca gagccgccgc cagtagccag cacaataccc
    19381 tgtttctcgg tcaactcatt gatgaccttt tcttcgcgat cgcggaagcc ttcttcgcct
    19441 tctaaatcga aaacccagcc cacatcagct ccggttcgtt tctcaatctc ttgatcggaa
    19501 tcgtaaaatt ccatattgag ttgttgagct aactggcgcc caatagtgct ttttccggca
    19561 cccataggcc caaccagaaa gatattgcgt ttctctgcca ttttttcggt actactaaga
    19621 ctattcgtta atgataaacc cgcttcgctc agagagcgcc gcaggacatg aactgaaacc
    19681 tcataagata ttgcgagagt cagactgaaa attatctcaa tactccagcg ggtttggcaa
    19741 ctgaataaat cacccatccc tctgcatatc tggtcgctgc aagcgcgctg ccttgctacc
    19801 accgctctgg cgataaatca ccgggtaaga ttagcgtaaa aaagacagca aaatgccgcc
    19861 tgaatgataa atcatcatca tggaacgcag gcagcgaaga gcaccaactc tcaaatcggt
    19921 actccttgta tgctaaatac ctgcgcgcgt caaatagatg aaacacgttc agcgtaaaaa
    19981 cattaccgct tttacggctg tttactcact ggaaaccagt cgtggcgtga taaacaccac
    20041 taactcgcgt cgttcatctt cttttccgtc atgacgaaat aattgcccga accagggaat
    20101 gtcgccaagc aacggcacgc tatcctgacc cgatttattt ttacgggtaa aaatgccgcc
    20161 cagcgccaac gtttctccgc ttttgacctc gacctgcgtt tcgatctcct gcttatcaat
    20221 cgccagcact tcgccatcgg cctgctgtag cacctgcccc ggaacgttct ggctgatgtg
    20281 taatttcagc cggatgcgac ctttttgtaa caccgtgggc gtgacctcca tccccaggac
    20341 tgcctcttta aattccaccg acgtcgcgcc actttccccg ctggaaactt gatatggaat
    20401 ttcgctcccc tgtttaatgc tggcaggctg aagatgtgag gccagcagac gcggactagc
    20461 gataatatcc agctgctgtt tttgttcgag cgcagaaagc tcaagatcca gcaaacgtcc
    20521 gttgatgcgc ccaatgttaa aaccgacatg cgttgtcgcc gtcgctacgg agaggtcgct
    20581 gccaagcgtg gtgacttgcc caacgccacc agcttgttgc gcatcggcca gcgtccattt
    20641 cacgcctaac tcacgcaaac ttttttcatt aatggtgaca atatgcgccg acagctcaac
    20701 ctgcccgacc ggcagatcca tttgcgctac ccactgttca agggcgctta acgccgtttt
    20761 gttatctcgt agcaaaaggc gattggtgcg tttatcgacg gtcatactcc ctttggtact
    20821 cagtagcttc tccccagctt tcgccagctc tccggcgtcg gcgtattgca gagtaatatt
    20881 gcgattttcc agcggcagat ttgcctgcgc ccgcgcctgc tccgcttcct gacgggcgat
    20941 attgtcattc tgccaggcaa tggaatgcac tgagagaatg ttgccctcct gacgcgttat
    21001 cagtccggcg cttttcacta cagtttgtag tgcctgcttc cagggaacat ctgttagatg
    21061 taacgacacc gtaccgctga cgtctggcga caccaccagg ttcaacttct cctgttcagc
    21121 cagcgcctgc aacacctgag ctaccggaac atcatccacc atcagcgtca ctttttgcgg
    21181 ctttgccgcc tgtacgccgg gtatcagcat caacagtagt gcggctatcc attgcttcat
    21241 ttgtatctcc ttgccgttgc cacaaccatc gtggcggttc gcagtttgtc ccggtaccca
    21301 gcgttagtac gtctggcgtc agctgtaaaa ttgtccagcc gttttccagc acatcgtttt
    21361 gctgcacccg tcgccatttc ttttgcccgt cttttattac accgatgatg cgctcgcctc
    21421 gccctaccat cccctgatag cgccactggc taagttcgct aatccggcat agatcttccg
    21481 gcggtttaaa agggtcacgc ataccggtta aaaggcacaa tgcaatacct gccaacaacc
    21541 agcgtttaac cctcatgcgg cgtctccagt tgtagcgtga acaaaagatc atcaccttcc
    21601 acgcttaatg aaaaacggct cacgctgacg ttgcgctctg ccagccgtgt aaatgccgac
    21661 ggcactgctt cccacagcgt tttcaacgcc aactcgcctc cctgcgcgga tggatgccag
    21721 aaaaccagtt gtgcgccggg caactgaaaa tccagtggcg aaaagggcag cgttttttct
    21781 tcgctgaagg gagtggtgtc aaccaggcga tacagtgtgg cccactgctg atgatgactt
    21841 acccgtagtc gaattaatgc gtcgcgctct tcatggtgtg tcgatgacag aaaaatgagc
    21901 gtaactaaca tcagcaacca gactgcccag caaagctggc ggagacgggg ggatgtggcg
    21961 aaccaccagt caaagaacat gttcatcgct aaccttcctt gttaactgat actcaaattg
    22021 ccagcgtccc tgcgcatcct gctgcgtggc cccccgctga ttgagatgaa aagatgcatc
    22081 ctggcggagt gacgtttcta gtgcgtttaa cgcggtaatg cttgttgtaa gccccttgat
    22141 ctccagcgtt ccctgctgcc agcttatcgt tgtcagccag gcgtgctcgg gtaaaagcgc
    22201 cgccagtgct tccagcgcag attgccagtc gcgggtaaat tgtcgctggc gctgcctttg
    22261 caaacgctgc tcacgaattt gttggcgctc cagcaaacgt ggtttcgtta tctgtaagct
    22321 gcgggcgagt tgttgttccc cctgaagcaa aacggcgtct acgcgagctt cggcgctgct
    22381 tgtcagacgc agtattagcg ttatcccgac ggccagcagc agaggcgcaa cgaacatcag
    22441 caaccagaaa cgaagaaaag cggtccggcg ttgctgtcgc cagggcaaaa aattaattgg
    22501 cgggttcatc agtacgcctt cccaagcgcc agccccagcg cgatggcaaa gtctccacct
    22561 ggcggcggta acggcggctg acgaacagaa acggcctccc agggatcaaa tccgccttcg
    22621 ccacatatcg cgacgctttc gggatcaacg gataacgctg ccgccagctc cttcgcgcta
    22681 gtcatcccca ccgccagttt gcgcccccag cgatagcgcg tcgcccacag ccactgttcg
    22741 ttatcacgcc aggccagaca ttgctgatga gaaggtaaaa aaggcaggaa tcgctgtaat
    22801 gcactggcat ccggggtgat cgcactaaca tgaacacgca acctttctgc cagagtaagc
    22861 agcgttgcca gctctttgct ttgcgcggca gtcacgttat aggcgggact gagtgagtct
    22921 tcgctgtaat cgaagcgcag ggagtccgga tccatatcca gctcgcgagc catcgtcccg
    22981 gacagccagg ccgtttgctc ccgctcaccg agggacatcg acgggcgcgg aaatgaccgc
    23041 tgtaatgtgc gactggcggg aaacgccaac ataatgtgat gacgctgcgg cagttcgcga
    23101 ctccacggta acaacgtttt agccagccgc tgcgcatcaa caatccgccc atctttgata
    23161 atgtcgtgcg ccaacggcaa ccgccaccag cgttgcaaaa agcattcttt tgcgccccgc
    23221 acaatagcaa ccgctaccgc ttcttgctgt tgtaaatgca aaccaatttg ccagatctta
    23281 aatgccattg tgatgatctc cttatcaccc gtcactctga cgggtatatc aatgcgtctg
    23341 gcttgccttt atactaccgc gcgtttgttt ataaactgcc caaatgaaac taaatgggaa
    23401 atttccagtg aagttcgtaa agtatttttt gatccttgca gtctgttgca ttctgctggg
    23461 agcaggctcg atttatggcc tataccgcta catcgagcca caactgccgg atgtagcgac
    23521 attaaaagat gttcgcctgc aaattccgat gcagatttac agcgccgatg gcgagctgat
    23581 tgctcaatac ggtgagaaac gtcgtattcc ggttacgctg gatcaaatcc cgccggagat
    23641 ggtgaaagcc tttatcgcga cggaagacag ccgcttctac gagcatcacg gcgttgaccc
    23701 ggtggggatc ttccgtgcgg caagtgtggc gctgttctcc ggccacgcgt cacaaggggc
    23761 aagtaccatt acccagcagc tggcgagaaa cttcttcctc agtccagaac gcacgctgat
    23821 gcgtaagatt aaggaagtct tcctcgcgat tcgcattgaa cagttgctga cgaaagacga
    23881 aatcctcgag ctttatctga acaagattta cctcggttac cgcgcctatg gtgtcggtgc
    23941 tgcggcacaa gtctattacg ggaaaacggt cgaccaactg acgctgaacg aaatggctgt
    24001 gatagccggg ctgccgaaag cgccttccac tttcaacccg ctctactcga tggatcgtgc
    24061 cgtcgcgcgg cgtaacgtcg tgctatcgcg gatgctggat gaaggatata tcacccagca
    24121 acagttcgat cagacacgca ctgaggcgat taacgctaac tatcacgcgc cggagattgc
    24181 tttttctgcg ccgtacctga gcgaaatggt gcgccaggag atgtataacc gttatggcga
    24241 aagtgcctat gaagacggtt atcgcattta caccaccatc acccgcaaag tgcagcaggc
    24301 cgcacagcag gcggtacgta ataacgtgct ggactacgac atgcgccacg gctatcgcgg
    24361 cccggcaaat gtgctgtgga aagtgggcga gtcggtgtgg gataacaaca agattaccga
    24421 tacgctgaag gcgctgccga cctatggtcc gctgctgcct gccgcagtca ccagcgccaa
    24481 tcctcaggaa gcgacggcga tgctggcgga cgggtcgacc gtcgcattga gtatggaagg
    24541 cgttcgctgg gcgcgtcctt accgttcgga tactcagcag ggaccgacgc cgcgtaaagt
    24601 gaccgatgtt ctgcaaacgg gtcagcaaat ctgggttcgt caggttggcg atgcatggtg
    24661 gctggcacaa gtgccggaag tgaactcggc gctggtgtcg atcaatccgc aaaacggtgc
    24721 cgttatggcg ttggtcggtg gctttgattt caatcagagc aagtttaacc gcgccaccca
    24781 ggcactgcgt caggtaggtt ccaacatcaa accgttcctc tacaccgcgg cgatggataa
    24841 aggtctgacg ctggcaagta tgttgaacga tgtgccaatt tctcgctggg atgcaggtgc
    24901 cggttctgac tggcagccga agaactcacc accgcagtat gccggtccaa ttcgcttacg
    24961 tcaggggctg ggtcagtcga aaaacgtggt gatggtacgc gcaatgcggg cgatgggcgt
    25021 cgactacgct gcagaatatc tgcaacgctt cggcttcccg gcgcaaaaca ttgtccacac
    25081 cgaatcgctg gcgctgggtt cagcgtcctt cactccaatg caggtggcgc gcggctacgc
    25141 ggtcatggcg aacggcggct tcctggtgga cccgtggttt atcagcaaaa ttgaaaacga
    25201 tcagggcggc gtgattttcg aagcgaaacc gaaagtagcc tgcccggaat gtgatattcc
    25261 ggtgatttac ggtgatacgc agaaatcgaa cgtgctggaa aataacgatg ttgaagatgt
    25321 cgctatctcc cgcgagcagc agaatgtttc tgtaccaatg ccgcagctgg agcaggcaaa
    25381 tcaggcatta gtggcgaaga ctggcgcgca ggagtacgca ccgcacgtca tcaacactcc
    25441 gctggcattc ctgattaaga gtgctttgaa caccaatatc tttggtgagc caggctggca
    25501 gggtactggc tggcgtgcag gtcgtgattt gcagcgtcgc gatatcggcg ggaaaaccgg
    25561 taccactaac agttcgaaag atgcgtggtt ctcgggttac ggtccgggcg ttgtgacctc
    25621 ggtctggatt ggctttgatg atcaccgtcg taatctcggt catacaacgg cttccggagc
    25681 gattaaagat cagatctcag gttacgaagg cggtgccaag agtgcccagc ctgcatggga
    25741 cgcttatatg aaagccgttc ttgaaggtgt gccggagcag ccgctgacgc cgccgccggg
    25801 tattgtgacg gtgaatatcg atcgcagcac cgggcagtta gctaatggtg gcaacagccg
    25861 cgaagagtat ttcatcgaag gtacgcagcc gacacaacag gcggtgcacg aggtgggaac
    25921 gaccattatc gataatggcg aagcacagga attgttctga ttaaaaaggc gcttcggcgc
    25981 ctttttaagt ttgctgacaa agtgcacttt gtttataccg gatacggcgt gaacacttta
    26041 cccgtctagc aacatctaga aaaatcaata tattgcagaa acttcgtaag cctgataagc
    26101 atcaggcaaa cttcacgcat ttacactcgc ccctgccctt tcaaccattc gcgcacgagg
    26161 aacagcgcgc tgacattacg cgcttcgttg aagtcagggt cttccagcaa atccatcata
    26221 tgcgccagcg gccagcgcac ctgtggtagc ggctctggct catcgccttc cagcgattcc
    26281 gggtagagat cttgcgctac cacgatattc attttgctgg aaaagtaaga cggtgccatg
    26341 ctgagcttct tcaaaaaagt cagatcgttc gcaccaaatc caacctcttc ttttagctca
    26401 cggttagcgg cttcgaagac gctttcaccc ggatcaatta atcctttcga aaaacctaat
    26461 tcgtaggatt cagttcccac tgcatattcg cggatcagga tcaggtgatc gtccacaatc
    26521 ggcacaatca tcactgcttc ccggttggtt ggacgcattc gttcataaac acgccgcacg
    26581 ccattgctga actccagatc cacgctctcg acggtaaaca gtcgggaacg ggctacagtt
    26641 tcaacattca gaatggtggg tttttgtaat gatttgctca tcgtgggatc tatgctgtga
    26701 aatcagcggt tattgtgcga tatcggacac gctttcggca atgtgaattg catgttattt
    26761 acatttatgt aacttaataa ataatcgtcc tcaaatcaaa ttaaaagtca ataggttgaa
    26821 ataactccag gaatttgctg atattccgcc ttcagagggt ttgctatgat cagcggttac
    26881 tgtgatgtgc ttaatgatgc tcaagttaaa ctccacgctt gccgatagcc aaccgcagaa
    26941 tcatgtattg tgtccggtgc gactgaccac gcctgacaga ctaagtaaga tggggaaagc
    27001 atgagcacca ttgtgatttt tttagctgct ttgctggcct gctcactact tgcgggatgg
    27061 ctgataaaag tgcgatccag acggcgtcag ctgccctgga ccaacgcctt cgcggatgcg
    27121 caaacgcgta aactcacacc tgaagaacgt agcgccgttg aaaattatct tgagagcctg
    27181 acgcaggtat tacaggtgcc tggcccaacg ggagccagcg cggcaccgat ctctctggcg
    27241 ctgaatgccg aaagcaacaa cgtcatgatg ctgacacacg ctatcacacg ttacggcatc
    27301 tctaccgacg atccgaataa atggcgttac tacctcgatt cggtagaagt ccacctgccc
    27361 cccttctggg aacagtacat caacgatgag aataccgttg aactgattca taccgattcg
    27421 ctgccgttgg ttatttcact caacggtcat acgctgcagg agtacatgca ggaaacgcgc
    27481 ggctatgcct tgcaacctgt tccgtccacg caggcgtcga ttcgcgggga agagagtgag
    27541 caaatcgagc tactcaatat tcgcaaagaa acgcatgaag aatatgcgct gagtcgtccg
    27601 cgcgggctgc gtgaagcgtt gctgatcgtc gcctccttcc tgatgttctt tttctgcctg
    27661 attaccccgg atgtatttgt tccgtggctg gcaggtggcg cgttactgct gctgggcgca
    27721 ggtctgtggg ggctattcgc ccccccggca aaatcctccc tgcgggaaat tcattgtctg
    27781 cgcggtacac cccgtcgttg gggattgttt ggcgaaaacg atcaggaaca gatcaacaat
    27841 atttcactcg gtattatcga cctggtctat cccgcacact ggcagccata cattgctcag
    27901 gatctcggtc aacaaaccga tatcgatatc tatctcgacc gccatgtagt gcgtcaggga
    27961 cgatatcttt cgctgcatga tgaagtaaaa aactttccgt tacagcactg gctgcgcagt
    28021 acgattatcg ctgcgggctc gctgctggtg ctgtttatgc tgttattctg gatcccgctg
    28081 gatatgccgc tgaaattcac tctctcatgg atgaaaggcg cgcagaccat tgaagccacc
    28141 agcgtaaaac aactggctga cgctggcgtg cgggtaggcg atacattgcg tattagtggt
    28201 acgggaatgt gtaatattcg cacttccgga acctggagcg cgaaaaccaa ttcacctttt
    28261 ttaccgtttg actgctcgca gatcatctgg aatgacgccc gctcattacc attaccagaa
    28321 tctgagctgg tcaacaaagc gacggcattg actgaagcag ttaatcgcca gctgcaccct
    28381 aaaccggaag atgaatctcg cgtcagtgcc tcattacgtt cagcgattca aaaatccggc
    28441 atggtattga ttgatgattt tggcgacatt gtactgaaga cagcggatct gtgttctgcc
    28501 aaagatgact gtgtgcgact gaaaaatgcg ctggtcaatc tcggcaacag taaagactgg
    28561 gacgcgctgg taaaacgcgc caacgccggg aagctcgatg gcgtgaatgt gttattacgc
    28621 ccggtgagtg cggaatcgct ggataacctg gtggcaacct ccaccgcgcc gtttatcacg
    28681 catgaaacgg cgcgagcggc acaatcacta aacagtccgg cccccggcgg attcctgatt
    28741 gtcagcgacg aaggcagcga ttttgttgat cagccctggc cttcggcatc actttacgac
    28801 tacccgccgc aagaacagtg gaacgctttc cagaaactgg cacaaatgct gatgcatacg
    28861 ccgtttaacg ccgagggtat cgtcacaaaa atcttcactg acgccaatgg tacgcagcat
    28921 ataggccttc atccgatccc ggatcgttcc ggcctgtggc gctatctcag caccacactg
    28981 ctgctactga cgatgctggg tagcgccatt tataatggcg tacaggcctg gcggcgttac
    29041 cagcgtcatc gcactcgcat gatgaagatt caggcctatt atgaaagctg cctgaacccg
    29101 caactgatca ccccttcaga aagccttatc gaataacacg tttgcgcggc aggttatgct
    29161 accctgtcgc gcaaattgct tcactctgga gattttcctc atgcatatca acattgcctg
    29221 gcaggacgta gataccgttc tgctggatat ggacggcacg ttgctcgacc tcgccttcga
    29281 taactatttc tggcaaaagc tggtgcctga aacatggggc gcgaaaaacg gggttacgcc
    29341 acaggaagcg atggaattta tgcgccagca atatcacgac gtacagcata cgctaaactg
    29401 gtactgtctt gattactgga gtgagcaact gggtctggat atctgtgcga tgaccaccga
    29461 gatgggaccg cgtgccgtac tgcgtgaaga taccattccg tttcttgagg cactgaaagc
    29521 cagcggtaag cagcgaattt tgctcaccaa tgcgcatccg cacaacctgg cggtaaaact
    29581 tgagcatacc ggtctggacg cacaccttga tttattactt tccacccaca catttggtta
    29641 tccgaaagag gatcagcggt tatggcatgc ggtggccgaa gctacgggtc tgaaagctga
    29701 aagaacgctg tttattgatg acagcgaagc gattctcgat gctgccgcgc aatttggtat
    29761 tcgttactgc ctcggcgtga ctaatcctga ctcagggatt gccgagaaac agtatcaacg
    29821 ccatccgtca ctgaatgact accgccgcct gatcccctcg ctaatgtgaa ggagaggcca
    29881 tgaaagaaaa acctgctgtt gaggttcgac tggataaatg gctatgggct gcccgttttt
    29941 ataaaacccg cgcgctggcc cgtgaaatga ttgaaggcgg taaggtgcat tacaacgggc
    30001 agcgcagcaa gccgagcaaa atcgtcgagc tgaatgccac gctcactctg cgccagggaa
    30061 atgacgaacg cacggtgatt gtaaaggcga ttactgaaca gcgtcgcccc gccagcgagg
    30121 cagccttgct gtatgaagag actgcggaaa gtgtagagaa acgcgaaaaa atggcgctgg
    30181 cacgtaaact taatgcctta accatgccgc acccggaccg acgcccggac aaaaaagagc
    30241 gccgcgacct gttacgattt aaacacggcg acagtgaata actgtcacct gcaagagaga
    30301 tgattatgcc gcaacatgac caattacatc gctatctgtt tgaaaacttt gccgtgcgcg
    30361 gcgaactggt aaccgtttcg gaaaccctgc aacagatcct tgagaaccac gattatccgc
    30421 agcccgttaa aaacgtgctg gcagaactgc tggttgcgac cagcctgtta accgctacgc
    30481 tgaagtttga tggtgatatc accgtacagc tgcagggcga cggtccgatg aatctggcag
    30541 ttattaacgg taacaataac cagcaaatgc gcggtgtggc gcgcgtgcag ggcgaaattc
    30601 cagaaaatgc cgacctgaaa acgctggtcg gcaatggtta cgtggtgatc accattaccc
    30661 cgagcgaagg cgaacgctat cagggcgtag ttggtctgga aggtgatacc ctggcggcct
    30721 gcctggaaga ttactttatg cgttctgaac agctgccgac gcgcctgttt attcgcaccg
    30781 gcgacgtaga cggcaaaccg gctgcaggcg gtatgttgtt gcaggtaatg cctgcgcaaa
    30841 atgcccagca ggacgacttc gaccacctgg cgacgctaac cgaaaccatc aaaaccgaag
    30901 aactgctgac cttaccggca aacgaagtgt tgtggcgttt gtatcacgaa gaagaggtga
    30961 cggtttacga tccgcaggat gtggagttca aatgcacctg ctcgcgtgaa cgttgcgccg
    31021 atgcgctgaa aacgctgcct gatgaagaag tcgatagcat cctggcggaa gatggcgaaa
    31081 ttgacatgca ttgtgattac tgcggtaacc actatctgtt caatgcgatg gatattgctg
    31141 aaatccgcaa caacgcgtct ccggcagatc cgcaagttca ttaatgagta tgtccggcag
    31201 agagagctct gccggatttt acgactaata cacctgatgc gctttgtcac tcattcagat
    31261 tcacatccag cctttcacca tccactacaa gatcgcgcca cgctttaggc ttcgttccca
    31321 gttttccatc cttcgcagct gtgagtaatt tttctttcgt tatatcacgc ggaagtaaac
    31381 tcatcgtaag ctcttgccat tctttcttgt caggatcaaa gccatagaca atgtatcttt
    31441 catcatcaaa ggcaaagagg atccgctctg ccctgccatc actattcaga tcttgctcga
    31501 ccagtacgca ggcgtctttt tcaatacagg tcatcacgtt atagcgatcc tggattaagg
    31561 ccgaccagaa tgtcgcatcc ggtttaccag aaccaggggc aattaacaca ttttcggcta
    31621 atactttttc cgatatctgt tgctgaagat gttgctctcc atctaacgcc atcagtagat
    31681 cccgcgcacg tttcgggtct ttcatatact cggcatcgct tttcagcgac tcaagcgccg
    31741 cacgtccata gcgaccgctg tgttcgagca tgtagatagt tacctggtct ggcgtgtttt
    31801 tgccgctctg ataacgcgcc atatgactgt tcacactaat gcgcatactg tccagcaccg
    31861 gcgaattaag aagcaccagt attaccaaca ccaataacga aaccgcaagg ttcactttgc
    31921 cctgaagaac aaggggattt tgccctttac gccagacgat actgacaaaa taccccagcg
    31981 accataccag taacaccagc accgccagcg cgccctgcaa gcggtcgaca gtccagccgt
    32041 actgagcgac ccgcagccat aacgcccagg cggcgatgaa cacatacagc ggagcaacca
    32101 gcagagcggt tttaatcagg caacgcaacg gccccgtcca gggaagtgac gctttttgcg
    32161 gatcgcggac aatagccatt aagatcaatt gcaaaaaggc cagcgttaac agcaacccgg
    32221 cggcggagat gtggcgagaa atcgcgctca ggcccgtaaa cggcagggtg atgataaaca
    32281 tcagggttag caatgatact aacggcagca accccgtggc gattagcgtg aacaactttt
    32341 gaatagagtc gattaaacgt gactgtgttc gcgcgaggat caccgccagc gcggtaacca
    32401 gacctaatgt aagatacata aaccagtcgg ttgcaaaaaa gagcgtattg aaaaacgtga
    32461 tgccaacaag tttaaacaac tcaccccaca gtaaaagcac cagccacgtc aggccattgg
    32521 cgagaaaaat aacctgtaat ataagtacgt tatgccatac tgactggtaa aaatagcaat
    32581 agcggctact gtcattgcgg atacgaaggc tttgttgtat ccacggtagc aacaacattg
    32641 ccatcagcag cagatagcag ccaaaatccc aaagcgcctt ttcagctctc caggggttca
    32701 tgccatcagt ttgccacttc aaccagccgc tcatccccag cgtggcaata aacaccagcg
    32761 ccagccatcc ccaaaggcgc ttctgtttaa aagagatcac ggagaagagt aaaacggatg
    32821 aaaacgcaac ggtcgcaggc acgccataaa caatccaact gtgatttttt ccggcaagcc
    32881 aggcgatcag cagatagcaa accagcccct gaagtaatcc ggtagcaatc atcccccagc
    32941 gtgttgcggg tgaaagttca acgttatcca tgttggttat ccagaatcaa aaggtgggtt
    33001 aattatcgca tccgggcagt agtattttgc ttttttcaga aaataatcaa aaaaagttag
    33061 cgtggtgaat cgatacttta ccggttgaat ttgcatcaat ttcattcagg aatgcgattc
    33121 cactcacaat attcccgcca tataaaccaa gatttaacct tttgagaaca ttttccacac
    33181 ctaaaatgct atttctgcga taatagcaac cgtttcgtga caggaatcac ggagtttttt
    33241 gtcaaatatg aatttctcca gatacgtaaa tctatgagcc ttgtcgcggt taacaccccc
    33301 aaaaagactt tactattcag gcaatacata ttggctaagg agcagtgaaa tgcgcgttaa
    33361 caatggtttg accccgcaag aactcgaggc ttatggtatc agtgacgtac atgatatcgt
    33421 ttacaaccca agctacgacc tgctgtatca ggaagagctc gatccgagcc tgacaggtta
    33481 tgagcgcggg gtgttaacta atctgggtgc cgttgccgtc gataccggga tcttcaccgg
    33541 tcgttcacca aaagataagt atatcgtccg tgacgatacc actcgcgata ctttctggtg
    33601 ggcagacaaa ggcaaaggta agaacgataa caaacctctc tctccggaaa cctggcagca
    33661 tctgaaaggc ctggtgacca ggcagctttc cggcaaacgt ctgttcgttg tcgacgcctt
    33721 ctgtggtgcg aatccggata ctcgtctttc cgtccgtttc atcaccgaag tggcctggca
    33781 ggcgcatttt gtcaaaaaca tgtttattcg cccgagcgat gaagagctgg caggtttcaa
    33841 accagacttt atcgttatga acggcgcgaa gtgcactaac cctcagtgga aagaacaggg
    33901 tctgaactct gaaaacttcg tggcgtttaa cctgaccgag cgcatgcagc tgattggcgg
    33961 cacctggtac ggcggcgaaa tgaagaaagg gatgttctcg atgatgaact acctgctgcc
    34021 gctgaaaggt atcgcttcta tgcactgctc cgccaacgtt ggcgagaaag gcgatgttgc
    34081 ggtgttcttc ggcctttccg gcactggtaa aaccaccctt tccaccgacc cgaaacgtcg
    34141 cctgattggc gatgacgaac acggctggga cgatgacggt gtgtttaact tcgaaggcgg
    34201 ctgctacgca aaaactatca agctgtcgaa agaagcggaa cctgaaatct acaacgctat
    34261 ccgtcgtgat gcgttgctgg aaaacgtcac cgtgcgtgaa gatggcacta tcgactttga
    34321 tgatggttca aaaaccgaga acacccgcgt ttcttatccg atctatcaca tcgataacat
    34381 cgttaagccg gtttcaaaag cgggtcacgc aacgaaagtt atcttcctga ccgcagacgc
    34441 ctttggtgtg ctgccgccgg tttctcgtct gactgccgat caaacccagt accacttcct
    34501 ctctggcttc accgccaaac tggccggtac tgagcgtggc atcaccgaac cgacgccaac
    34561 cttctccgct tgcttcggcg cggcattcct gtcgctgcac ccgactcagt acgcagaagt
    34621 gctggtgaaa cgtatgcagg cggcgggcgc gcaggcttat ctggttaaca ctggctggaa
    34681 cggcactggc aaacgtatct cgattaaaga tacccgcgcc attatcgacg ctatcctcaa
    34741 cggttcgctg gataatgcag aaaccttcac tctgccgatg tttaacctgg cgatcccaac
    34801 cgaactgccg ggcgtagaca cgaagattct cgatccgcgt aacacctacg cttccccgga
    34861 acagtggcag gaaaaagccg aaaccctggc gaaactgttt atcgacaact ttgataaata
    34921 caccgacacc cctgcgggtg ccgcgctggt agcggctggt ccgaaactgt aatgacttga
    34981 agctggagaa tatctatcca gtatcttata gaaagcaaaa cgggaggcac cttcgcctcc
    35041 cgtttattta cccttctttt gtcgtgccct gcgcccgcgt taccggcaca ggcagccagg
    35101 cgcgaatgga aagcccgccc cgctcgctgg tgccaagctc cagcatcccg ttatggttat
    35161 ccacgatacg ctgcacaatc gccagcccta atcccgtgcc gctaatggtg cgtgcactgt
    35221 cgccacgaac aaacggctgg aaaaggtgct tacgttgttc cggcgcaatt cccggaccgt
    35281 catcttccac ctggaaccag gcgcgattcg gctccgtccc gctgctgact ttgatccagc
    35341 cattgccgta acgggcggcg ttgaccacca tattcgccac cgcgcgtttg atcgacagcg
    35401 ggtgcatttt cacttcaatg ctgccggggt aaagcgcggt ttcaatttcc cgctcatagc
    35461 cactttcggc agcaataacc tcaccgagta ctgcgttgag atccgccatt tccatcggca
    35521 tctcctgccc ggtgcgcagg tagtcgataa actgctcaat gatggcgttg cactcttcga
    35581 tatctttatt gatcgattct gccaggtagc catcctgctc gctcatcatc tcggtcgcca
    35641 ggcgaatacg cgtcagcggt gtacgcaagt cgtgacttac ccccgccatc agcagcgtgc
    35701 ggtcatccgc cagttgctta acaccagccg ccatatggtt aaaggcgcgg gtaacggaac
    35761 gcacctcgga cgcgccatac tcacgcagcg gcggcggaat aatcccttta ccgacctgca
    35821 aagcagcatg ttcgagatcg accaacggtc ggttctggat acgaataaac agccacgccc
    35881 cgcctatcgc caatagcata atcgccaacg tatagcggaa cagcggagag aaatcgccct
    35941 gatgaatttc ggtcagcggc acgcgtaccc agatattggg cgacagccaa gttttcagcc
    36001 agacgacagg cgaacttttg ttgacctcaa cgcgcacttc cgtcgggccg cccagttgct
    36061 gcgccatctg atggcttaag aattcatagt gttgcgccca acgcagacct gcctcttcgg
    36121 cagcctcgtt ggagtagaga gagatcccca gctcacggta gatctcccga cggaaagcgg
    36181 gaggcacaac caactgcgtg ccgtcctcca gttgcagttt gtcggtcatc aacatacgca
    36241 cttcgtacgc gaggacttta ttaaactgct ggaggctcgg caaaatcgcg aagttcagca
    36301 ccaccagata agtcgtcacc aggctggcga acagcaaggt gacgatgagc aataacgtac
    36361 gggcaaatga acttcgtggc gagaagcgca atcgcctcat gctttagagc cgtccggtac
    36421 aaagacgtag cccaagcccc aaacggtctg aatgtaacgc ggatgcgctg gatcttcttc
    36481 caccatgcgg cgcagacgcg aaatctgcac gtcgatggag cgttccattg cggaatattc
    36541 acgaccacgg gcaaggttca tcagcttatc gcgggagagc ggctcacgcg gatggctgac
    36601 cagtgccttc agtaccgcaa actcaccgct ggtgagcggc atcggctcgt cttcgcggaa
    36661 catttcgcgc gtaccgaggt taagtttgaa cttaccgaaa gcaattaccg cctcttcctg
    36721 tgacggtgcg cctggtagct cgttcgcctg acgacgcagc accgcacgga tacgggccag
    36781 cagttcacgc gggttaaacg gttttggaat gtaatcgtca gcgccaatct ccaggccaac
    36841 gatacggtcc acttcttccc ctttcgccgt caccataatg atcggcatcg ggttgctctg
    36901 actacgaaga cgtcggcaaa tcgacaagcc atcttcacca ggtaacatca aatccagtac
    36961 cataagatgg aaagattcac gagtcagcag gcgatccatc tgttctgcat tagcgacgct
    37021 tcgaacctgg aagccttgtt cggtgagata acgctccagc agcgcgcgca ggcgcatgtc
    37081 gtcatcgacc accagaatct tgtagttctc ttgcattgtt tgtactccca aaggttcgca
    37141 acaatttgta agcgtgtatt cttaaaaaag ctcacgttcg tcaccagcta aatctggtat
    37201 gaatttcagc ctaaattgtt acaaagcata ttaaacagca gcttaagtat acaatttatt
    37261 cggcgaaaca ttattgattc tgttgatatg atcacgttat acccaatgtg cgcattatca
    37321 aacagacaaa gggaatcaac gagatgaaaa cgcccctggt tacccgggaa gggtatgaaa
    37381 aactcaaaca agagcttaat tatctctggc gtgaagaacg cccggaggtc acaaaaaagg
    37441 tgacctgggc cgcaagtctg ggcgaccgca gcgaaaatgc tgactatcag tataataaaa
    37501 agcgtctgcg tgaaatcgac cgtcgcgtgc gctatctcac taaatgcatg gaaaatctca
    37561 aaatcgtcga ttactcccct cagcaggaag gcaaagtctt ttttggcgcg tgggtggaga
    37621 ttgaaaacga cgatggcgtg actcaccgtt tccgtattgt cggctacgat gaaatttttg
    37681 gccgtaaaga ttacatctct atcgattccc cgatggcccg cgcattgctg aaaaaagaag
    37741 tcggcgatct ggcggtggtg aatacccctg ccggggaagc gagctggtat gttaatgcta
    37801 tcgagtacgt gaaaccgtaa ggaagagtct taacctcctg ccgatggctg gcatttttgc
    37861 cagccagtcc gtataactat cccctgattt ttgatccgaa aagatgaact caaaccatga
    37921 tgaatgattc gttctgccgc attattgcgg gtgaaattca ggcgcgcccg gaacaggttg
    37981 acgctgccgt tcgcctgctt gacgaaggga ataccgtgcc ttttattgca cgttatcgta
    38041 aggaaatcac cggcggtctg gatgacacgc agctgcgtaa tctggaaacg cgtctgagct
    38101 atctgcgcga gctggaagag agacgtcagg cgatcctcaa gtccatttcc gagcaaggca
    38161 aactcaccga tgatctggcg aaggccatca acgccacctt aagcaaaacc gaactcgaag
    38221 acctctacct gccctacaaa cctaaacgcc gcacccgcgg gcaaatcgcc attgaagcag
    38281 ggcttgagcc gttggctgac ctgctgtgga gcgatccgtc acacacgcca gaagtcgccg
    38341 ctgcgcaata tattgatgca gataaaggcg tggcagatac caaagccgcg ctggacggcg
    38401 cgcgctatat cctgatggaa cggtttgccg aagatgccgc gctgctggcg aaagtgcgtg
    38461 attatctgtg gaagaacgcg catttggttt cgacggtggt gagcggtaaa gaagaggaag
    38521 gggcgaaatt ccgcgactat ttcgatcatc acgaaccgtt gtccacggtg ccttctcacc
    38581 gcgcgctggc gatgttccgt gggcgtaacg aaggcgtact ccagctttcg ctgaatgccg
    38641 atccgcagtt cgatgagccg ccaaaagaga gctattgcga gcaaatcatc atggatcatc
    38701 tcggcctgcg cctgaacaat gctccggcgg atagctggcg caaaggcgtg gtgagctgga
    38761 cctggcgcat caaggtgctg atgcatctgg aaaccgaact gatgggcacc gtgcgcgaac
    38821 gcgcggaaga tgaagcaatc aacgtctttg cccgtaacct gcacgatctg ctgatggcgg
    38881 cccctgccgg actgcgtgca acgatgggcc tcgatccggg tctgcgtact ggggtaaaag
    38941 tggcggtggt cgatgccact ggcaaactgg tagcgaccga taccatttac ccgcacaccg
    39001 gacaagccgc aaaagcagcg atgactgttg ctgcgctgtg tgaaaagcat aacgttgaac
    39061 tggtggcgat cggcaacggt acagcttccc gcgaaaccga acgtttctat ctcgacgtgc
    39121 agaagcagtt cccgaaagtg accgcacaga aagtgatcgt cagcgaagct ggcgcgtcgg
    39181 tttactcggc ttccgagctg gcagcgcagg agttcccgga tctcgacgtt tcgctgcgtg
    39241 gcgcggtttc tatcgcccgc cgtttgcagg atccgctggc ggagctggtg aaaatcgatc
    39301 cgaaatctat cggcgtaggt cagtatcagc atgacgtcag ccagacgcaa ctggcccgca
    39361 aactggatgc agtagtagaa gactgcgtaa acgccgttgg cgtcgatctc aacaccgctt
    39421 ctgttccgct attaactcgc gtggcgggcc tgacgcgcat gatggcgcaa aacatcgtgg
    39481 cctggcgcga tgagaacggc cagttccaga accgccagca actgctgaaa gtcagccgtc
    39541 tggggccgaa agccttcgag cagtgcgcgg gcttcctgcg cattaaccac ggtgacaacc
    39601 cgctggacgc ctctaccgtt cacccggaag cctatccggt ggtggaacgc attctggcag
    39661 caacacagca ggcactgaaa gatctgatgg gtaacagcag cgaactgcgt aacctgaaag
    39721 cgtctgactt tactgatgaa aaattcggtg tgccgacagt aactgacatc atcaaagagc
    39781 tggaaaaacc gggtcgcgat ccgcgtccgg aatttaaaac cgctcagttt gccgatggcg
    39841 tcgagacaat gaacgacctg caaccgggta tgatcctcga aggcgctgtc accaacgtca
    39901 ccaactttgg cgcgtttgtc gatatcggcg tgcatcagga cggcctggtt cacatctctt
    39961 cattgtcgaa caagtttgtg gaagatccgc ataccgtggt gaaagcgggc gacattgtga
    40021 aggtgaaagt gctggaagtg gatcttcagc gtaaacgtat cgccctgact atgcgtctgg
    40081 acgagcagcc tggcgaaacc aactctcgtc gcggcggcgg taacgatcgc ccgcaaaaca
    40141 accgcccggc agccaaacca cgcggtcgtg aagcgcagcc tgccggtaac agcgcgatga
    40201 tggacgcgct ggcggcagca atgggcaaaa aacgttaaac gcccggactg gcccctaaac
    40261 ctgcgcgata aggcgttcac gccgcatctg gcaacgaacg ccttgcctga cataaaagtg
    40321 ccggagaata tctccggcat atttattcca cagccaaact cacaatatat ttcgttaata
    40381 tttatcactt cattaacaac tgaaacctta attaaacatt agccagtcct ggtaattcgc
    40441 tattcgaatt atattttcgc tgcgatataa ccttgagcca catcaacatt gagtcagatt
    40501 attattcaaa ccaacattcg cacacatttt aagtattgct gatagaaacc attctcatta
    40561 tcattgtact gttgattatt taatctctcc ttcgttggca aatcatctgg tctcatgtcg
    40621 ctgtcaaacg ccccatgagg tagttatcca gttaatgaga aacaagtagg cacctatgca
    40681 atacactcca gatactgcgt ggaaaatcac tggcttttcc cgtgaaatca gcccggcata
    40741 tcgccaaaaa ctgctttctc ttggcatgtt acctggctcc tcttttaatg tggtgcgcgt
    40801 cgctccactc ggcgacccca ttcatatcga aacccgtcgt gtgagcctgg tattacgcaa
    40861 aaaagatctg gccttattag aagtggaagc ggtttcctgt taatacggtg ataacaacaa
    40921 tgaaaaaatt aaccataggc ttaattggta atccaaattc tggcaagaca accttattta
    40981 accagctcac tggcgcacgt cagcgtgtag gtaactgggc tggcgttacc gtcgaacgta
    41041 aagaagggca attctccacc accgatcatc aggtcacgct ggtggacctg cccggcacct
    41101 attctctgac caccatctca tcgcagacct cgctcgatga gcaaatcgcc tgtcactaca
    41161 ttttgagtgg cgacgccgac ctgctgatta acgtggtgga tgcgtctaac cttgagcgta
    41221 acctgtacct gacgctacaa ctgctggaac tcggcattcc ctgcatcgtg gcactgaaca
    41281 tgctcgacat tgccgagaag caaaatattc gtattgaaat tgatgctctg tcggcgcgtc
    41341 ttggctgccc agtgatcccg ctggtttcaa cccgtggtcg cggtattgaa gcgctcaagc
    41401 tggcgattga tcgctataaa gctaacgaga atgtggaatt ggtgcattac gcacagccgc
    41461 tgctcaacga agcagattca ctggcaaaag tgatgccttc cgacatcccg ctgaaacaac
    41521 gtcgctggct gggcctgcaa atgctggaag gcgatatcta cagccgcgcc tacgccggtg
    41581 aagcgtcgca gcatctggat gccgccctcg cccgtctgcg taatgagatg gacgatccgg
    41641 cgctgcacat tgccgatgcg cgttaccagt gcattgctgc catctgtgat gtggtaagca
    41701 acaccctgac ggcagaaccc agccgtttca ccactgcggt agataaaatc gtgctcaacc
    41761 gtttcctcgg tctgccgatt ttcctctttg tgatgtacct gatgttcctg ctggctatca
    41821 acatcggcgg ggcgttacag ccgctgtttg acgtcggctc cgtggcgcta tttgtgcatg
    41881 gtattcaatg gattggctac acgctccact tcccggactg gctgactatc ttcctcgccc
    41941 agggcctggg tggcggtatt aacaccgtgc tgccactggt gccgcagatc ggcatgatgt
    42001 acctgttcct ctccttcctt gaggactccg gctatatggc gcgtgcggcg tttgtgatgg
    42061 accgcctgat gcaggcgctg ggcttgccgg ggaaatcctt tgtgccgctg atcgtcggtt
    42121 tcggttgtaa cgtaccgtcg gtaatgggtg cacgtacact tgatgcaccg cgtgaacgtc
    42181 tgatgaccat catgatggca ccgtttatgt cctgcggcgc gcgtctggct atcttcgcag
    42241 tattcgcggc tgccttcttc gggcagaacg gtgcgctggc ggtcttctcg ctgtatatgc
    42301 tcggtatcgt gatggcggtg ctgactggcc tgatgctcaa gtacaccatc atgcgcggtg
    42361 aagcgacgcc gtttgtcatg gagctgccgg tctaccatgt accacacgtt aaaagcctga
    42421 ttatccagac ctggcagcgc ctgaaaggct tcgttctgcg tgctggtaaa gtgattatca
    42481 tcgtcagcat tttcctgagc gctttcaaca gcttctcgtt gagcgggaaa atcgtcgata
    42541 acatcaacga ctcggcgctg gcttccgtca gccgggtgat caccccggtc ttcaagccga
    42601 ttggcgtgca tgaagataac tggcaggcaa cagttggcct gtttacgggt gcaatggcga
    42661 aagaagtggt ggtgggtacg ctcaacaccc tctacaccgc agaaaatatt caggacgaag
    42721 agttcaatcc ggcggaattt aacctcggtg aagagttgtt cagcgcggta gatgaaacct
    42781 ggcagagcct gaaagacacc ttcagcctta gcgtactgat gaaccccatt gaagctagca
    42841 aaggcgacgg cgaaatgggt accggggcga tgggtgtgat ggatcagaaa ttcggtagcg
    42901 cagctgccgc atacagctac ctgattttcg tcctgctgta tgtaccgtgc atctcggtga
    42961 tgggagccat cgcccgtgaa tcaagccgtg gctggatggg cttctccatc ctgtgggggc
    43021 tgaatatcgc ttactcgctg gcaacattgt tctatcaggt cgccagctac agccagcatc
    43081 caacttacag cctggtgtgc attctggcgg ttatcctgtt taacatcgtg gttatcggtc
    43141 tgctgcgccg cgcgcgtagc cgggtggata tcgaactgct ggcaacccgc aagtcggtaa
    43201 gtagttgctg cgcagccagc accaccggtg attgccatta atggcttcac ttattcaggt
    43261 gcgtgatttg ttggcgttac ggggccgtat ggaagcggcc cagataagca agacattgaa
    43321 cactccacag ccaatgatta acgccatgct gcaacaactg gaaagtatgg gcaaagccgt
    43381 gcggattcag gaagaacctg acggctgcct ctccggcagt tgtaaaagct gcccggaagg
    43441 aaaagcctgt ctgcgcgagt ggtgggcgct gcgttaacca tactccatcg cctgttttga
    43501 aaagcccggt atgcgtttgc atccgggctt ttttgcgtgc ggctttccat aaaaatgcaa
    43561 ttcttgcagc gcgacgtaag ttcctctgaa agcgtctcgc agggatgaaa actggctaat
    43621 gcacaggtgt ggagtggcgc gtagagtcgc ggcattcaaa caacaggtga aggaacgcca
    43681 tgagcaaaaa gcagagttcc accccacacg atgcgctgtt caaactattt ttacgccaac
    43741 cggagacggc acgcgatttt cttgcgtttc atttaccggc acccattcac gcgctctgtg
    43801 atatgaaaac cctcaagctg gagtcgagca gctttattga tgacgatctg cgtgaaagct
    43861 attccgacgt gctgtggtcg gtgaaaacgg aacaaggacc aggatacatc tattgtctga
    43921 ttgaacatca aagcacctca aacaaactga tcgcattccg catgatgcgt tacgccattg
    43981 ccgcaatgca aaatcacctt gatgccggat acaaaacgtt gccgatggtg gtgccattgt
    44041 tgttttatca cggtattgaa agcccctatc cctattcgct gtgttggctg gattgtttcg
    44101 ccgatcccaa cctggcaagg cagctctatg cctctgcatt tccgctgatt gatatcaccg
    44161 tcatgcctga tgatgaaatc atgcagcacc gacgcatggc gctgctggag ttaattcaaa
    44221 aacatattcg tcaacgcgac ctgatggggc tggtagaaca aatggcctgc ttattaagta
    44281 gtggatacgc taatgacaga caaatcaaag ggctgtttaa ttacatactg caaaccggcg
    44341 atgcggtacg ttttaacgat tttatcgacg gcgttgccga acgttcaccg aaacacaagg
    44401 agagtttaat gactattgcg gaaagattgc ggcaggaggg ggaacaatcc aaagccttgc
    44461 atatagccaa aataatgctt gaatccggag tccctcttgc agacatcatg cgctttaccg
    44521 ggctgtcaga agaagagttg gctgcggcga gtcagtaaag ttctgtctcg ccattcaaaa
    44581 gtgcctacac cctctgcttc aacgccacca gcaggtgaca aaactcgacc ggatgcgaaa
    44641 taaacggcgc atgggccgct ttggcgaaga tatacgactc gctgtgcggc cagagtttat
    44701 ccagcatcgg caccactttg cgcggcacca gaccgtcgag ataaccatac agtcgcaaaa
    44761 acggcatggg tacgttttgc agcggctggc ggagatcgac cgttttcagg atttccagcc
    44821 caccattgag cacgtcaacc tccggcatcg gtaacgccag aacggttttc ttcaacgccc
    44881 gcgcatcctg acgcgccgtt tcagtaccca tggtttgtaa cgcaaggaac cgttccaccg
    44941 tacgctgaaa atcatcactg agttgctgct gaaatcccgc cagcacgtcc ggttttatcc
    45001 ccggccactc ttcacgagcg ctgaaacacg gcgacgacgc cacggtgacc agcgcctgaa
    45061 cacgctcggg atgggttaac gcaatctggc ttgccaccag cccgcccaga ctccagccta
    45121 accaaatggc tttatcaggt gcctgtcgca gcacggcttc ggccatatca gcaagtgaca
    45181 tcgcaccaaa tccccggcta cgcccgaagc cgggcaggtc aacaagatgc agcgtaaaat
    45241 gcgagctaag ttcctcgtca atgcaacgcc acacttcggc attcagtccc catccgtgca
    45301 gcagcacaag atgaacattc ccctgacctt tggtctgcca ccagatgtta ttcatccgct
    45361 attgttctct tttgacttac aaggatgaac atatgctaac agtaccggga ttatgctggc
    45421 tatgccgaat gccgctggct ttaggtcatt gggggatttg ttcggtctgc tcacgcgccg
    45481 cccgtacaga taaaacgtta tgcccacaat gtggattacc cgccacacac tcccatcttc
    45541 cctgcggtcg ctgcctgcaa aaaccgccgc cctggcaaag actggtcacg gttgccgact
    45601 atgcgccgcc gttaagtctg ctcatccacc agcttaagtt ttcccgccgc agcgaaatcg
    45661 ccagcgccct gtcacgtctg ttactattgg aagtcttaca cgctcgtcgc accaccgggt
    45721 tgcaattgcc ggatcgtatc atcagcgttc cgttatggca gcggcgtcac tggcgtcggg
    45781 gatttaatca gagcgatttg ctgtgtcagc cgttatcacg ctggttgcac tgccaatggg
    45841 atagcgaagc catcacacgt acacgggcca ctgcgaccca gcattttctt agcgcccggc
    45901 tacgcaggcg caacctgaaa aatgcctttc gccttgaatt gcctgtgcaa ggtcgccata
    45961 tggtgattgt ggatgatgtc gttaccaccg gaagtaccgt cgcagagatt gcgcagttgc
    46021 ttttacgcaa tggtgcggcg actgtccagg tctggtgcct ttgtcgaacc ttgtagagcc
    46081 tcgatgatgg gcgtattata accaactaaa atagtcaact attaggccat tactatgatc
    46141 cgtatttccg atgctgcaca agcgcacttt gccaaactgc tggcaaatca ggaagaaggg
    46201 acacaaatcc gcgtatttgt gattaaccct ggcacgccta acgctgaatg tggtgtttct
    46261 tattgtccgc cggatgccgt ggaagccacc gacaccgccc tgaaatttga cctgctgacc
    46321 gcgtatgttg atgagttaag cgcaccatac ctggaagatg cagagatcga ttttgttacc
    46381 gaccagttgg gttctcagct aacactgaaa gcgcctaacg ccaaaatgcg taaagtggca
    46441 gacgatgcac cactgatgga gcgcgtggag tatatgctgc agtcgcagat caacccacag
    46501 cttgctggtc acggtggccg cgtttcactg atggaaatca ccgaagacgg ttacgccatt
    46561 ctgcaatttg gcggcggctg taacggttgt tccatggtcg atgtgacgct gaaagaaggg
    46621 atcgagaagc agctactaaa cgaattcccg gagctgaaag gtgtgcgcga tctcaccgaa
    46681 caccagcgcg gcgaacactc ctactactaa gttatcccct cttttgtaga ttgcccgatg
    46741 cgacgctaaa gcgtcatttc gggcctacgt tgaacatgtg ccagacgttg gcgcagcatt
    46801 gctttggcaa atccctccct tcgtttcccc gcgataatat gaccaacctc tcataattta
    46861 aatttacccc gctctggtga ttctcaaacg ccagatgtta cccgtatcat tcacatgggt
    46921 accaaacata ctcctgacat ctgactacaa taattagttt tagtgggtat cagtcgtggt
    46981 gccgcaatat ctctgttccc gattgggata attagagttt gtcgtcagaa aattgacgtt
    47041 acccataaca aatgaaaggc caggtaaatc atgccattag tcattgttgc tatcggtgta
    47101 atcttgttgt tgctcctgat gatccgcttc aaaatgaacg gcttcatcgc tctcgtcctc
    47161 gtggcgcttg ctgttggatt aatgcaagga atgccgctgg ataaagttat tggctccatc
    47221 aaagccggtg tcggcgggac gctcggtagc cttgccctga tcatgggttt tggcgcaatg
    47281 ctgggcaaaa tgctggcaga ctgcggtggc gcacaacgta tcgccaccac gctgattgcc
    47341 aaatttggta aaaaacacat ccagtgggcg gtggtactga ccggttttac cgttggtttt
    47401 gccctgttct atgaagtggg ctttgtgctg atgctgccgc tggtgtttac catcgcggct
    47461 tctgcgaata ttccactgct gtatgttggt gtaccaatgg cggctgcact gtctgtgacc
    47521 cacggcttcc tgccaccgca tccgggtccg actgcgattg ccaccatttt caatgccgat
    47581 atgggtaaaa ccctgctgta cggtactatt ctggcaatcc cgaccgtgat tctcgccggt
    47641 ccggtttacg ctcgcgtgtt gaaaggtatc gataagccaa ttccggaagg tctctacagc
    47701 gcgaaaacct tcagcgaaga agagatgccg agctttggcg tcagcgtctg gacctctctg
    47761 gtgccggtag tactgatggc gatgcgtgcg attgccgaaa tgatcctgcc gaaaggtcac
    47821 gctttcctgc cggtagcgga gttcctcggt gacccggtaa tggctacgct gattgccgtg
    47881 ctgattgcga tgttcacctt tggtctgaac cgtggtcgtt caatggatca gattaacgac
    47941 acgctggttt cttccatcaa aatcattgcg atgatgctgt tgatcatcgg tggtggcggt
    48001 gcgttcaagc aggtgctggt agacagcggc gtggacaaat acattgcttc catgatgcac
    48061 gaaaccaaca tttctccgct gctgatggcc tggtcgattg ctgccgtact gcgtatcgcg
    48121 ctgggttccg caaccgttgc ggcaatcact gcgggtggta tcgcggcacc gctgattgca
    48181 acgacgggtg ttagcccgga gctgatggtt attgcggttg gttccggtag tgtgattttc
    48241 tctcatgtga acgatccggg cttctggctg ttcaaagagt actttaacct gactatcggc
    48301 gagaccatca aatcctggtc gatgctggaa accattatct cggtgtgcgg tctggtaggc
    48361 tgtctgctgc tgaatatggt gatttgataa cactgactgc cggatgcatc gcatccggca
    48421 tcaaccgcac tctacttctt cttcgctgca gctctgcgcc gtctgtccaa atccttcagc
    48481 aacttgttca cgccatcatc ggcaaacatc gtctcaagcg ttgcggaaag cttgcgtcgc
    48541 cagtttttat actggtaact ggtgccagga atattcaccg gttcggccat atccagccag
    48601 tcttccggct gtagtcctaa cagagcactg ttactgtcgg caatgtagcg ctgcaaacca
    48661 cggttcagcg tcggcgtcat cgacatcaac gatgccttat gcccggcacg ttttggcaga
    48721 caaccatatt tatgcagtgc atccagcagc ccttgcttcg ccagttcgcg atcctgatac
    48781 agaccgcgca gtaccacttc atccggatac agccccaggg ttttgcccag agttagatcc
    48841 ccgctctccc agtaaccacg cagcgttggc aggtcgtgtg tcgccgcaac cgccatcgac
    48901 tgctccgggt acgctttcgg tgcacggaac gtcttctcgt ggtcgttttc gaaatagagc
    48961 actttgtagg agtacacacc gctgctgcgc agcttaccga caatctctac cggtacggta
    49021 ccgagatctt caccaatcac catacagcga tgacgtttac tttcgagtgc cagaatcgag
    49081 agcagatcat ccaccggata gtgaacatac gcgccctgat ctgccgtctc gccatacggt
    49141 atccaccaca aacgcagcat cgacataaca tggtcaattc gtaatgcgcc gcagttttgc
    49201 atattggcac gcaacagctc gataaacggt tcataggcac gcgcggtgat gatatgcggg
    49261 tccattggcg gcaatcccca gttctgcccc aacgggccga ggatatccgg cggcgcgcca
    49321 accgacgctt tcaggcaata cagttcacgg tcgcaccagg tttccgcccc accttccgct
    49381 acgccaaccg ccagatcacg atacaagcca atcggcattt catagccctg gcttatctcc
    49441 cagcaggcgg caaactggct gtaagccagc cactgcagcc agagataaaa atcaacgtca
    49501 tcgcgatgtt cttcgcagaa ctgacgcact tctggtgaat ccacgttctg atacatctct
    49561 ggccatgcag gccagcccca gcgcatttcg tcctctttca cttgctgggc atgtagcgca
    49621 tcaaaggctg cctgccagaa caggctgtcg ccttgatctg caacaaactg gcgaaacgcg
    49681 gccatctgct catcatcacg ttgcgcgaaa cctttccacg ccattcgtaa tgctgtcatt
    49741 tttagggcgg taaccgtgga gtaatcgacc cagtcggcat cccgcgcctg ttgcagcgtc
    49801 tgttgcgtgg tcggcaactg ccaccaggcc tgagcctctt cgctaagatg gaaatcttcg
    49861 acggcgttaa cgtcgatata aatcacattc agccagcggc gagaagacgg gctgtatggg
    49921 ctggcgctct ccggatttgc cggatagagc gcatgaatcg ggttcaggcc gatgaacgac
    49981 ccgccacgtt ttgccacatc caccagcatc gctttgagat cgccaaaatc cccaataccc
    50041 cagttttttt ccgatcgcag cgtataaagc tggacgcagg caccccacag cttttgttta
    50101 ttcagcaacg cctgcggttc gtaacagcgt ttcggggcga caatcaccct gcaatgcgcg
    50161 cgctggtctt cctgggtaag cgtcagcgtg tgataacctt ccggcagctt cgtcggtaga
    50221 ttgaacgctt tgcccccagt tacatggcct ttgtactgcg ttccttcttc ggtggtcagc
    50281 agccagctat attcgccgct gccctccacc accatcggca tttttttgcc gctggtataa
    50341 accatgacat tcgggactgg cgttaccgcc actttcgtgg cggtacgttg atgcatcgcg
    50401 tcaagcaaac gccgtttggt ttcggcgcta atcgactgcg gtttaccgtg ggcattgatg
    50461 taattggggc taatccccgc cgccagcgcg gcattatcca gacgtttgct ttccatcgag
    50521 cttccttagc gttttgcctg ccagatacga gcctgataat cgcgaataga gcgatccgag
    50581 ctaaacatac cgcagcgggc ggtattgagg atcgccgcgc gagtccaggc ctcctggtcg
    50641 cggtacagca catccacctg cttttgtgcc tctacatagg ctgcgaaatc cgccatcacc
    50701 agatacggat cgccgccctg tttgccgata ctgtgcagca tctggtcgaa ggcatgctta
    50761 tcgccgtcgc tgtatttacc gctttccagc tctttcaata ctgcgtccag caccttatct
    50821 ttcttccgcc atttcaccgg gtcgtagcct ttggccagaa ttgccttcac ttgttccacg
    50881 gtatgaccaa aaataaagat attttcttca ccgactttct cggcgatttc aacgttcgcc
    50941 ccatccagcg taccgacagt aagcgcacca ttgagcgcca gtttcatatt gccggtaccg
    51001 gaagcttctt tacctgcagt cgaaatttgt tcggagatat ccgccgccgg gatcagtttt
    51061 tccgccgccg aaacgcaata atccggcagg aacaccacct tcaacttatc gccaaccagc
    51121 ggatcgttgt tgatcacgtc agccactttg ttgatcgcaa agataatatt cttcgccagg
    51181 tagtagcccg gtgccgcttt cgcgccgaag aggaagacgc gcggtacgcg atcagcctgc
    51241 gggttttcac gaatttcttt gtacaacgcc agaatatgca gcagattcag gtgctggcgt
    51301 ttgtactcat gcagacgttt gatctgaata tcgaaaatcg cctgtggatt gatctcaata
    51361 ccggtacgaa ctttcacaaa ctccgccaga cggactttat tcgcctgctt gatctcgcga
    51421 tattgctgac ggaatttcgc atcatcagcg aatttttcca gattgatcag ctgatcgaga
    51481 tcgttagccc actctttttt cagtgattta tccaacagag ccgccagtgc cgggttgcac
    51541 tgtttgatcc agcgacgtgg ggtaataccg ttggtgacgt tatggaattt gttcggccat
    51601 agctggtgat attccgggaa cagatctttc accaccagat ccgagtgcag cgccgcaaca
    51661 ccgttcaccg cgaaaccgcc aaccacacac aggttcgcca tatgcacttg tttgtcgtgc
    51721 accaccgcca gtttggccca cactttttca tcgcccggcc aggttttctc caccagcgtt
    51781 ttaaagcgag tattaatttc gttaataatc tgcatgtggc gcggcagtaa gcctttcacc
    51841 agtttcacat cccagcgttc cagcgcttct ggcatcaggg tatggttggt gtaagcgaaa
    51901 gttttgctgg taatggccca ggcgtcatcc cagctcatct ggtgctcatc gatcagcacg
    51961 cgcagcagtt ctggaatcgc gatagttggg tgggtatcgt tcagctgaat aacttcgtaa
    52021 tccgccagtt cgtgcagttt acgccccgcc agatgatggc gacgcaaaat atccgctacc
    52081 gaacaggcac actggaagta ctgctgcatc aagcgcagct ttttaccggc agtatggttg
    52141 tcgtttggat agagaacttt ggtcagtttt tccgcattga tgccctgctg ttcggcacgc
    52201 aggaaatcac cgtcgttaaa tttagtcaga tcaaacggat gcgcgtgcgt cgcctgccac
    52261 agacgcagcg gctgcgccac gccattacga tagccgacaa cggggagatc ccacgcttga
    52321 ccggtaatgg taaactccgg ctcccagcgt ccgtctttcg tcactttacc gccaatccct
    52381 acctgcacat ccagtgcttc gttgtggcgg aaccacgggt agttactgcg atgccagtca
    52441 tccggcgctt caacctgttt gccatcgaca aaagactggc ggaacaaacc atattgatag
    52501 ttcagaccgt aacccgtcgc agactgaccg acagttgcca ttgaatcgag gaagcacgcc
    52561 gccagacgtc ccagaccacc gttacccagc gccgggtcga tctcttcttc cagcaggtca
    52621 gtcagattga tgtcataagc cttcaacgaa tcctgtacat cctgatacca gccgagattc
    52681 aacaggttgt tgcccgtcag acgaccaatc aaaaactcca ttgagatgta gttaacatgt
    52741 cgctgattcg ccaccggctt ggcgaatggc tgagcacgca gcatttcggc cagtgcttca
    52801 ctcactgcca gccaccactg gcgaggagtc atttcagccg cagaatttaa gccataacgc
    52861 tgccactgac gtgaaagcgc ttcctgaaat tgcttatcgt taaaaatagg ttgtgacata
    52921 ggagttccac ttttcttaga ttttcaacac aacgttatcg ctagtttgcc aggctcgatg
    52981 ttgaccttcc tcatcctgcg ggggattagg cagggaggag ttgcggggat gagcaaggaa
    53041 atgtgatctc aaccacttaa agctagtgca aaccacagga ttagcagcaa atcaatgcca
    53101 tacagcgcag aaaatctgta tctaagtgca aaaaatggcc gttgcgtatt ttcaaaaagc
    53161 ggaaggtaac tctataaatt aagtaaagga gtgaaacagt ttcacaagta aaatatccag
    53221 tgtgctccat ctcattctta atagatttat taagatcatc tttttagata gcactttcat
    53281 caggaatgaa gaagaaaccc ttgcttaaat gaatctgatg aacataaggg aaaccagtat
    53341 tcacgctgga tcagcgtcgt tttaggtgag ttgttaataa agatttggaa ttgtgacaca
    53401 gtgcaaattc agacacataa aaaaacgtca tcgcttgcat tagaaaggtt tctggccgac
    53461 cttataacca ttaattacga agcgcaaaaa aaataatatt tcctcatttt ccacagtgaa
    53521 gtgattaact atgctgattc cgtcaaaatt aagtcgtccg gttcgactcg accataccgt
    53581 ggttcgtgag cgcctgctgg ctaaactttc cggcgcgaac aacttccggc tggcgctgat
    53641 cacaagtcct gcgggctacg gtaagaccac gctcatttcc cagtgggcgg caggcaaaaa
    53701 cgatatcggc tggtactcgc tggacgaagg tgataaccag caagagcgtt tcgccagcta
    53761 tctcattgcc gccgtgcaac aggcaaccaa cggtcactgt gcgatatgtg agacgatggc
    53821 gcaaaaacgg caatatgcca gcctgacgtc actcttcgcc cagcttttca ttgagctggc
    53881 ggaatggcat agcccacttt atctggtcat cgatgactat catctgatca ctaatccagt
    53941 gatccacgag tcaatgcgct tctttattcg ccatcaacca gaaaatctca ccctggtggt
    54001 gttgtcacgc aaccttccgc aactgggcat tgccaatctg cgtgttcgtg atcaactgct
    54061 ggaaattggc agtcagcaac tggcatttac ccatcaggaa gcgaagcagt tttttgattg
    54121 ccgtctgtca tcgccgattg aagccgcaga aagcagtcgg atttgcgatg acgtttccgg
    54181 ttgggcgacg gcactacagc taatcgccct ctccgcccgg cagaataccc actcagccca
    54241 taagtcggca cgccgcctgg cgggaatcaa tgccagccat ctttcggatt atctggtcga
    54301 tgaggttttg gataacgtcg atctcgcaac gcgccatttt ctgttgaaaa gcgccatttt
    54361 gcgctcaatg aacgatgcac tcatcacccg tgtgaccggc gaagaaaacg ggcaaatgcg
    54421 cctcgaagag attgagcgtc aggggctgtt tttacagcgg atggatgata ccggcgagtg
    54481 gttctgctat cacccgctgt ttggtaactt cttgcgccag cgctgccagt gggaactggc
    54541 ggcggagctg ccggaaatcc accgtgccgc cgcagaaagc tggatggccc agggatttcc
    54601 cagcgaagcg attcatcatg cgctggcggc aggcgatgcg ctgatgctgc gcgatattct
    54661 gcttaatcac gcctggagtc tgttcaacca tagcgaactg tcgctgctgg aagagtccct
    54721 taaggccctg ccgtgggaca gcttgctgga aaatccgcag ttggtgttat tgcaggcgtg
    54781 gctgatgcaa agccaacatc gctacggcga agttaacacc ctgctagccc gtgctgaaca
    54841 tgaaatcaag gacatcagag aaggcaccat gcacgcagaa tttaacgctc tgcgcgccca
    54901 ggtggcgatt aacgatggta atccggatga agcggaacgg ctggcaaaac tggcactgga
    54961 agagctgccg ccgggctggt tctatagccg cattgtggca acctcggtgc tgggtgaagt
    55021 gctgcactgc aaaggcgaat tgacccgctc actggcgcta atgcagcaaa ccgaacagat
    55081 ggcacgccag cacgacgtct ggcactacgc cttatggagt ttaatccagc aaagtgaaat
    55141 tctgtttgcc caagggttcc tgcaaaccgc gtgggaaacg caggaaaaag cgttccagct
    55201 gatcaacgag cagcatctgg aacagttgcc gatgcatgag tttctggtgc gcatccgagc
    55261 acaactgtta tgggcctggg cacggctgga tgaagccgaa gcgtcggcgc gtagcgggat
    55321 tgaagtcttg tcgtcttatc agccacagca acagcttcag tgcctggcaa tgttgattca
    55381 atgctcgctg gcccgtggtg atttagataa cgcccgtagc cagctgaacc gtctggaaaa
    55441 cctgctgggg aatggcaaat atcacagcga ctggatctct aacgccaaca aagtccgggt
    55501 gatttactgg caaatgaccg gcgataaagc cgccgctgcc aactggttgc gtcatacggc
    55561 taaaccggag tttgcgaaca accacttcct gcaaggtcaa tggcgcaaca ttgcccgtgc
    55621 acaaatcttg ctgggcgagt ttgaacctgc ggaaattgtc ctcgaagaac tcaatgaaaa
    55681 tgcccgcagt ttgcggttga tgagcgatct caaccgtaac ctgttgctgc ttaatcaact
    55741 gtactggcag gccggacgta aaagtgacgc ccagcgtgtg ttgctggacg cattaaaact
    55801 ggcgaatcgc accggattta tcagtcattt tgtcatcgaa ggcgaagcga tggcgcaaca
    55861 actgcgccag ctgattcaac tcaatacgct gccggaactg gaacagcatc gcgcacagcg
    55921 tattctgcga gaaatcaatc aacatcatcg gcataaattc gcccatttcg atgagaattt
    55981 cgttgaacgt ctgctaaatc atccagaagt gccagaactg atccgcacca gcccgttgac
    56041 ccaacgtgaa tggcaggtcc tggggctgat ttactccggt tacagcaacg aacaaatagc
    56101 gggtgaactg gaagtggcgg caaccaccat caagacgcat atccgcaatc tgtaccaaaa
    56161 actcggcgtc gcccaccgtc aggctgccgt gcagcacgcg cagaaactgc tgaagatgat
    56221 gggttatgga gtgtgagttt agccggataa tgcattggat ccggcttaca tctttatatc
    56281 attcaatact cacccgcgtt acgccatccg tttctatcaa accaaaccgc accggcaaga
    56341 aacgctccac caccgcgata ttggtcagca gatggcatga gggatgggcg accgtaaatt
    56401 gtcccgcgcc cgccagcgcc attggcaaca ctagctggtc agcaagatat tcccccactg
    56461 ccgccgggct tgccaggtag cgtttcacct ctttcaccaa ctgtgccgca accacctcgg
    56521 cactgacgcg cttttcaccg acgacaaaaa agcgttcggt gatattttca ctttcgactt
    56581 caagcgagac ggtattaccc ggcccctggt cgcgcggcag gttatgaata tactgctcat
    56641 gcaggggaaa actccccgcc agtgtagcga tttcacgctc agcaacatgg cgcggcacac
    56701 cagccagtag cacctctcca cgcatctgca caatgttccc gcgctcgcca agttgcaagg
    56761 ttttaaacga tgccactggc gagacttctg ttgccaccac gccgcctccg gcagggtaaa
    56821 aaccgtgacg taacagcgtg gtttgctgat gaattcctat tttcgccagc agcggctcca
    56881 acacccggcg gataaaatcc gcaggcgggg ccgacgggtt atcggtaccg ccgctcactt
    56941 caacacgcga aggtccatcg gcaaaccaca gcgcaggcag caccgtttgc agcaccagcg
    57001 tacaacttcc tgcgctaccg atagcaaagc ggtaatcgcc gccacgcacg gtgccgggcc
    57061 ggaagaccag acgctgcgac cccagctccg cgccttccac cgttgcccta caaatttccg
    57121 cagctgcttt taccgcggtc agatgctggc gcaacagtcc cggttttgcc cgcccggcac
    57181 gaatgccggt gatggtaaat ggctgaccgg ttatcatcga cagactcagc gccgagcgca
    57241 ggatctgccc gccaccttcg ccctgtgcgc catccagcgc aatcatcctt ttcatcattt
    57301 atccttttac gcacaccacc tgacgcaggg tatagataac ttccaccaga tcgctttgtg
    57361 ccgccatcac cgcatcaata tctttatacg ccatcgggat ttcgtcgatc acttcggcat
    57421 ctttacggca ttccacatgc gcggtggcac gaatttgatc ttccacgctg aacatttttt
    57481 tcgctttagt acggctcata acccgcccgg caccgtggct gcacgaacag aacgactctt
    57541 catttcccag cccacggacg ataaagcttt tcgctcccat cgaccctgga ataattccat
    57601 attgaccagc acgcgcagac accgcgcctt tacgcgtcac gtagatctct tcgccaaagt
    57661 gctgttcttt ttgcacatag ttgtggtgac agttgatctc ttccattgcc agcgtttgtg
    57721 gctgtcttac cgttttctgc gtaatgctct gcaacgccgt taccacgttt tccatcatcg
    57781 catcgcggtt aaggctggca aaaagctgcg cccaggccac cgctttcagg taatcatcaa
    57841 agtattccgt accttccata aagtacgcca gatcacgcga cggcaacgtt tcaagctggt
    57901 cctgcatctc tttttgcgcc agatcgataa aataagttcc gatggcgtta ccaattccgc
    57961 gtgaaccgga gtgcagcata atccacacct gatccgaccc atcaaggcag atttcaataa
    58021 agtggttacc cgttcccagc gttcccaggt gtttatagtt gttagtgtta aggaaacgcg
    58081 gatatttttg cgttaaccac tgataaccgg cctccagctc agcccatttc gcatcgacgt
    58141 taacaggcgg gttttcccat gcgcctttat cacgtttaca acggccagtg gtacgcccgt
    58201 gcggcaccgc agtttcaatc gcctgacgca gctccgccag gttttccggc aggtcagccg
    58261 ccgttaaagc ggtacgtagt gcgttcattc cacaaccgat atccacgccc accgccgccg
    58321 gaataatcgc ccctttggtc gggatcacgc taccaatggt ggaaccttta cccaggtgta
    58381 catcaggcat taccgcaata tgtttgaaaa taaacggcat cttcgccgta ttaataagtt
    58441 gctgacgcgc atcggcttct accggcacgc ctttggtcca catttttacc gaggcatttt
    58501 cagtggtcag taattcgtaa ttcattttgt ttttctcttt tcgttggtat gtcctgataa
    58561 ttgcaaccgt cgtgccagaa aattaaataa gcagattaat tttttaattc attgttttaa
    58621 aaaagattat atctttacgt ccgtaaccgg agatttcccg caaagccgat ttaccgataa
    58681 tgaaatatcg tcttttataa ggatatctaa gatgcgtaaa acagtggctt ttggctttgt
    58741 cggcaccgta ctggattatg ccggacgcgg cagtcagcgc tggtcgaaat ggcgtccgtc
    58801 actctgtata tgccagcaag aatcgttagt catcgatcga ctggaattat tgcacgacgc
    58861 ccgctcgcgc tcgctgtttg aaacgcttaa acgcgatatc gtcagcgttt cgccagaaac
    58921 agaagtggtg agcgttgaga ttgagctgca taacccgtgg gatttcgaag aggtttacgc
    58981 ctgcctgcat gatttcgccc gtggttacga gtttcagcca gaaaaagaag actatttaat
    59041 tcacatcacc accggcaccc acgtcgcgca gatttgctgg tttctgctgg cggaagcgcg
    59101 ttacctgccc gcccggttga tacaatcttc acctccgcgc aaaaaagaac agccgcgcgg
    59161 cgcaggtgaa gtgacgatta tcgatctcga tttaagccgt tataacgcca tcgccagccg
    59221 ctttgccgag gaacgccagc aaacgcttga ttttcttaag tccggtattg ccacgcgtaa
    59281 cccccacttc aaccgcatga ttgagcagat tgaaaaagtg gcgattaaat cccgcgcacc
    59341 gatcctgcta aacggcccaa ccggtgcggg caagtcattt ctggcgcgac gtatatttga
    59401 attaaaacag gcgcggcatc agtttagcgg cgcgtttgtg gaagtgaact gcgcaaccct
    59461 gcgcggcgat accgccatgt cgacgctgtt tggtcatgtg aaaggcgcgt ttaccggggc
    59521 gcgggaatct cgtgaaggat tattacgcag cgccaacggc ggaatgttgt ttcttgatga
    59581 gattggcgaa ctgggcgcag acgaacaggc aatgctgctt aaagccattg aagaaaaaac
    59641 cttttatccg tttggcagcg atcgccaggt gagcagtgat tttcagctta tcgccggaac
    59701 ggtgcgtgat ttgcgccagc tggttgccga aggaaaattt cgcgaagacc tgtacgcgcg
    59761 gatcaatctc tggaccttca ccctgccggg gctacgccag cgtcaggaag atattgaacc
    59821 taacctggat tatgaagtgg agcgccacgc ttcactcacc ggcgacagcg tgcgttttaa
    59881 caccgaagcg cggcgcgcct ggctggcctt tgcgacatct ccccaggcaa catggcgcgg
    59941 taactttcgc gagctttctg ccagcgtcac gcggatggct acctttgcca ctagcggacg
    60001 catcactctg gaagtggtgg aagatgagat aaaccgtctg cgctataact ggcaggagag
    60061 tcgcccctcc gcgcttacgc agctactggg cgcagaggca gaaaacatcg atctcttcga
    60121 ccgtatgcaa ctggaacacg ttatcgctat ctgccgccag gcaaagtcac tttctgcagc
    60181 cggacgacag ctttttgatg tttcgcgcca gggcaaagcc agcgtcaacg acgcggatcg
    60241 gctacgcaaa tacctggcgc gttttggtct gacgtgggaa gcattgcagg atcagcacag
    60301 ctccagttga atatggtggt ccgtcagcac ctgcatcacg cttactggcg gcggggcatc
    60361 ggtgtagacg gcatctacca tgctgatgct gcccatattg accatcgcgt tacggccaaa
    60421 tttcgagtga tcgacaacca gcataacgtg gcgcgagttc tcaataatgg cgcgtttggt
    60481 gcgaacttcg tggtaatcga actccagcag cgagccgtcg ctatcgatgc cgcttatccc
    60541 cagaatgccg aaatcaaggc ggaactggga gataaaatcg agcgtcgctt cgccaatgat
    60601 cccgccatcg cggctgcgta attcgccacc ggcgagaata atacggaaat cctcttttac
    60661 catcagcgtg ttagcaacgt taagattgtt ggtgacaatg cgcaaattgc tgtgattaag
    60721 cagcgcgtgc gccaccgctt ccggcgtggt gccgatatcg ataaacagcg tcgagccgtt
    60781 ggggatttgc tccgccactt tacgggcgat gcgctctttt tcttcggtct gggtggcttt
    60841 acgatcgtgc cacggcgtgt taaccgaact ggaaggcagt gccgcaccgc catgatggcg
    60901 cagaatcagg ttttgctccg ccagctcatt gagatcgcgg cgaatagtct gcgggctgac
    60961 ggagaagtgc tccaccagct cctcggtgct gacataaccc tgctgtttaa ccagttcgat
    61021 aataccgttg tgacgttgtg tttgtttcat ttataaatcc ctggaattat tttcgttttc
    61081 gcgcattgag cgaatcaaca aaagccatcg ctaaacccac ggctaacccg gcgatgtgtg
    61141 ctccgttcgc catcgacatc ccaaacaaat caaaccatcc ggcgacaatc cagatcagcg
    61201 caaagataat taacccacgt tgcaggtaaa tgccactttg cggatcgcgt tcgccacgta
    61261 gccagacgta gcccatcagc gcatacacca cgccagaaag cccgccaaac cacggcccgc
    61321 tgaatttttg ctgcacatag ccgcttaaca gggcgctaat gagagtaata acaattagct
    61381 taccgctacc gaggcgtttt tccaccgcgc cgccgagata ccaccaccag agcaggttaa
    61441 agaggatatg catcagcgag aagtgcatta acgcgtgggt gaagtaacgc cagaactcaa
    61501 atttcagtgc tggatcgaat ggccaggcca gccataacat cacttcctga tcgccgagaa
    61561 tttgcatggc aataaacacc accacgcagg cgatcatcat cacccaggtt accggacctg
    61621 cgcgttcacg caaggcggca aagaaaggat aacggcgata atgcaggcca ctgccggtat
    61681 ggcctgcctg ccagctcgcc gccagataac gcggatctgc cgggttttcg agaaaacgcg
    61741 ccagctccgc ccgtacgcgc tcggcctggg actcatccgc cagccagaca tcgctttggt
    61801 tatgttgttg aatcgtgagg ataacaccct gcgtcgccat gtaatcaaca aacgcctgcg
    61861 ccacgcgggg gttagcaaaa gaggtaatca tcaacatcgt tgctgtcgct tattccacac
    61921 aaaaggggac agtataaagc gttacgcgcc gtacgccacc tctgcgggaa actgacgttg
    61981 ccaggcttca aagccgccgt caatgctata gaccacatcg tagccctgtt gcagcagata
    62041 ctgcgccgcg cctttgctgc tattgccgtg ataacacatc accatcaccg gagtgtcaaa
    62101 gtcgttatta cgcataaaag cgcccagcgt gtcgttggtt aaatggaaag cctgcaccgc
    62161 atgtcccatt gcgaaactct gtggatcgcg aatatcgacc agcaccgcct ctttttcctg
    62221 caacttctgg tgcgcgtcgg caacgttaat acattcgaac tgatccatgc gtctctcttt
    62281 ctttacaaac aagtgggcaa atttaccgca cagtttacgt cgaagcggca gataaacgcc
    62341 ataatgttat acatatcact ctaaaatgtt ttttcaatgt tacctaaagc gctattcttt
    62401 gctaatatgt tcgataacga acatttatga gctttaacga aagtgaatga gggcagcatg
    62461 gaaaccaaag atctgattgt gatagggggc ggcatcaatg gtgctggtat cgcggcagac
    62521 gccgctggac gcggtttatc cgtgctgatg ctggaggcgc aggatctcgc ttgcgcgacc
    62581 tcttccgcca gttcaaaact cattcacggt ggcctgcgct accttgagca ctatgaattc
    62641 cgcctggtca gcgaggcgct ggctgaacgt gaagtgctgc tgaaaatggc cccgcatatc
    62701 gccttcccga tgcgttttcg cctgccacat cgtccgcatc tacgcccggc gtggatgatt
    62761 cgcattggtc tgtttatgta cgatcatctg ggtaaacgca ccagcctgcc gggatcaact
    62821 ggtttgcgtt ttggcgcaaa ttcagtgtta aaaccggaaa ttaagcgcgg attcgaatat
    62881 tccgactgtt gggtagacga tgcccgtctg gtgctcgcca acgcccagat ggtagtgcgt
    62941 aaaggcggcg aagtgcttac tcgtactcgt gctacttctg ctcgtcgcga aaacggcctg
    63001 tggattgtgg aagcggaaga tatcgatacc ggcaaaaaat atagctggca agcgcgcggc
    63061 ctggttaacg ccaccggccc gtgggtgaaa cagttcttcg acgacgggat gcatctgcct
    63121 tcgccttatg gcattcgcct gatcaaaggc agccatattg tggtgccgcg cgtgcatacc
    63181 caaaagcagg cctacattct gcaaaacgaa gataaacgta ttgtgttcgt gatcccgtgg
    63241 atggacgagt tttccatcat cggcaccacc gatgtggaat acaaaggcga tccgaaagcg
    63301 gtgaagattg aagagagtga aatcaattac ctgctgaatg tgtataacgc gcactttaaa
    63361 aagcagttaa gccgtgacga tatcgtctgg acctactccg gtgtgcgacc actatgtgat
    63421 gatgagtccg actcgccgca ggctattacc cgtgattaca cccttgatat tcatgatgaa
    63481 aatggcaaag caccgctgct gtcggtattc ggcggtaagc tgaccaccta ccgaaaactg
    63541 gcggaacatg cgctggaaaa actaacgccg tattatcagg gtattggccc ggcatggacg
    63601 aaagagagcg tgctaccggg tggcgccatc gagggcgacc gcgacgatta tgccgctcgc
    63661 ttacgccgcc gttatccgtt tctgaccgaa tcgctggcac gtcatttcgc gcgtacttat
    63721 ggcagcaaca gcgagctgct gctcggcaat gcgggagcgg taagcgatct tggggaagat
    63781 ttcggtcatg agttctacga agcggagctg aaatatctgg tcgaccacga gtgggtccgc
    63841 cgcgccgacg atgccctgtg gcgtcgcaca aaacaaggta tgtggctaag tgcggaacaa
    63901 caatctcgtg tgagtcagtg gttggtggag tatacgcagc agaagctgtc gctggcgtcg
    63961 taaattaacg taaggtgatc aggtcagatt tcaatctggc ctgagactga tgacaaacac
    64021 aaaattgcct gatgcgctac gcttatcagg cctacgtggt ttatgcaata tattgaattt
    64081 gcatgatttt gtaggcctga gaaggcattc acgccgcatc aggcatgaac aaagtgccac
    64141 gttgtcaaca atctgaccta ctttttgatt cagggaatta ccgcaaagcc cacgccatca
    64201 tacccaacgt aacaaaactt aagcacgttc cccactcact ccacttccgt atctggcatc
    64261 tatttttgct gcgccactgg caataatatc ccaacgcaaa accaagctgt gcgcttattg
    64321 gaccgataaa atccgtccac ggctgaggcc aaaatggcga aaaattcagg ggtagccaga
    64381 caacaagcag aaagaaattg cggatacttt ccagcaacgc aattttcgct ggcgcgatgt
    64441 aaaaaatgat attccagagg atcaaccata aatagaccgg tgcgaatacc aaccaacaat
    64501 ggagcctgct tttcaattca gattgagttc tacgagtaaa ttgccaacca acgcaacaca
    64561 ttacacagaa gaaaagcgca tattcagccg ataaccaaag ttgtaattcc agaagggaaa
    64621 tacgcctgtg catgaacgaa tttgagaaca gcatctccat tagaaaactc agtgtgatac
    64681 caacaacaac caccgtcggc cacaccaaca acggcacaat aacaaagccg agatatagcg
    64741 gataaatata aagtctgatt ttattcatct ttattgtcct gattattcgg gtgatgcgtc
    64801 atacatcgcg cataaacact catcaatcat ccagtcgtct tcccagactt gatattctgt
    64861 cattggttca ggctgtttta gtgggttctc aatcgcttgt cgcgctcttt ctttcagcaa
    64921 aggatgatta tttctagccc aggtattaat ttgagtggta atagttttat tatcatcact
    64981 ctgctcaaaa tcgctatttt cgacgtattc tttactgtta cgccagagca aacatgccat
    65041 agacgccgtc ggccagtaac cggaaatgga cataatggcg tcaagtaagg gttcaatctt
    65101 tataccaccg cgataaaaca tttctaatat tatttccagt tccacgacct ccttagccga
    65161 cacccaatcc tgcatatata aataggcgaa atcgctaatt atttgtagtt catccgttcg
    65221 ccagttctgg ttatctgcct ctgctattct ctgtaaacag cagccttcat aaatgcctgg
    65281 aaattgcctt tgggcaataa attcaaacaa tctgggtaat aaatagcgaa tctcatcatc
    65341 attttgcggg gtaggaccaa tacttgttcc ccaggcatgt aataaaggga aaggtatggc
    65401 acgcagtgaa acccctctta aggcttgttg ttgttcttcg ctaaagcagt aaggacaaca
    65461 aacatgaaat ctcgacggca ccggataacg acgaaaaata tgataaatct gctcaattaa
    65521 tgcctgcatg gcggggctgc attgcataat tactccctct tccgctgatt gcgtgtaaac
    65581 ggttgataaa caaaacgagc attggccgcg ttcaggcgaa taataccggt atccatgtga
    65641 ttatcctgct taaggtaagg ttttatacat cggcacataa cctacatctt ccaccaaact
    65701 ctgcccctgc ggcgttaaaa accattccac caatttttgt gtctctgacg tcgtattttc
    65761 cctcgttacc ataaaggcat cgatgatata gggatatttg ccattgcgaa tattttccgc
    65821 agtcggtgca ataccgttaa tcgctagcaa ttttatattt ttatcagcat tcatttgcgt
    65881 cgcgtaatag cggaaggtat agcctattgc gttgtttgta ttacggtatt cggcaacgac
    65941 tttaatcatt ccctccatca cgcttgccac ttccgtttcc tgcggcgaga tcatgcggac
    66001 atttttcatg acctgtgatt gcatcactgt ctggctgcca gagtcttccg ggcgctgcca
    66061 ggtctggatc tcctgatcgt taccgccaac agtacgccag ttggtaattg caccactgaa
    66121 aatgtcacgc acctgttgtt cagtcatgga attaaccgga ttatccgcat tgacgatgaa
    66181 aacgaatgct tcacgggcaa agggggtgta cagcaaagtg acaccagatt cctttgcacg
    66241 ttttttttgc ccaccggaag gttgcgccac gaaaataata tcagcatcgc ccttaacgat
    66301 tttgttatat gcttctggcg tacgagagtt ttccagataa tcccaaacgt gaaaatcctc
    66361 tggtattaca cttaatgcat aaaatgcaga agcataaatt gggtacgcag ccgtggcgcc
    66421 atcgattcgc ggccagtttt gcctaaactg aatttgcggt ttgccacgca gcggcgtcag
    66481 tcgattattg agtttatcgg gttgccaggc ccagatatca atattttcgc gtacaccgac
    66541 accaggattg agctgtttat ataaataacc ctgataacca gcgacaaaac caagcatgag
    66601 aaggataaac aggagggaat tacggcaacg tagcccggat gtaccggtta aaggatgtct
    66661 ggttggccgg ccgtacccca gcgaaaaact gatatgcgcg ccaacgggta taatgactat
    66721 ccaccagtac tcacctccga ctactgagac taacaaaacg cccagccagg ggagcataag
    66781 atcagccaac caacccgatc ccatctggtg aaattgataa tctgccagat gcagcgcgat
    66841 tatccagcca accagggtta atagcgcggg taagagaaaa aagcattgcc gccagacggc
    66901 tatttctctg ttttctggcg cgctatcacc ggcccacacg cgaccagaga aaaatattat
    66961 tatcgccccc agcgtcagca taaagacgtt gagtagaaga taaattgttg gcgtgataag
    67021 gatattacaa acttcaataa taccggtaag cccgacaccc agcatggcaa caaccgctgc
    67081 caaaaattgc gccagtatgg ggatgccgaa aaaagtcatt atcagcgagg tcaaaatcca
    67141 ttttctattt ggcatcattc tgtccattct tcctgaatga tggaattata ctccccgaga
    67201 accctcgccc cctctggaca cttttccgaa atgatggcga aaaaaacggg accctttggc
    67261 cccgttctat ttattggtga aattacaatc tcaccggatc gatatgccag atatgatcag
    67321 cgtactcttt gatagtacgg tcagaagaga agtagcccat attggcaatg ttcagcatcg
    67381 cttttgcggt ccactcttcc tgaagctcgt aaagttcgtc aactttatcc tgacaatcga
    67441 catagctgcg ataatccgcc agtacctggt agtgatcgcc gaagttgatc agcgaatcaa
    67501 ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg atttgcgtca
    67561 gcacctgatg cagctcctca tctttctcat agtattcacg cggtttgtag ccctgacgac
    67621 gcagttcttc cacttcttcc gctgtgttac caaaaataaa gatattgtca gcaccgacat
    67681 gatccagcat ctcgacgttt gcaccgtcca gcgtaccgat agtcagcgcg ccgttaagcg
    67741 caaacttcat gttactggtg ccggaagctt ccgtccccgc cagcgaaatc tgttcagaca
    67801 gatcggccgc cggaatgatc aactgcgcca ggctaacgct gtagttcggg atgaacacga
    67861 ctttcagctt gtcgccaatc tgcggatcgt tgttgatcac tttcgctacg tcattgatca
    67921 aatggataat atgcttcgcc atgtaatagg ccgaagccgc cttaccgcca aaaatattca
    67981 cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg gtaatcacat
    68041 gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgt acatcgaaca
    68101 acgccttagg attcaccacc acattcagct gctgggcgat atactccgcc agacgctttt
    68161 tgttctccag cttcgcctga tgcacagcgt gattaaccat tgggaaatca cagtgttgtt
    68221 gcagctcatt aagcaggcta aggtcggtgc gccagttacg gcccaggtgt tcgtccagca
    68281 cggctgaaag cgatgggttc gctaccgcca gccagcgacg cggcgtcaca ccgttggtga
    68341 cgttggtgaa acgacccggg aagattttcg caaagtcggc aaacaacgat tgcaccatca
    68401 gattagagtg cagttccgat acaccgttaa ctttgtggct cacaacaacc gccagccagg
    68461 ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc agcagatcgg
    68521 tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt tcaaagatga
    68581 tctgcaggtg acgcggcaga attttaccca gcatatcaac cggccaggtt tccagcgcct
    68641 cgctcatcag cgtgtggtta gtgtaggaga agacctgaca acacacctca aacgcgtcgt
    68701 cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc gacagtaccg
    68761 gatgggtatc attgagatga atcgcgattt tatccgccag gttatcgtag gttttatgca
    68821 actgataatg gcggcttaaa atgtcctgaa tggtcgagga aaccaggaag tattcctgac
    68881 gcaggcgcag ctcacgcccg gagtaggtgg agtcatccgg atacagtacg cgagatacgt
    68941 tctcggagtg gtttttatct tccactgccg cgaagtagtc accctggttg aatttaccga
    69001 ggttaatttc gctactggct tgcgcactcc acaaacgcag cgtgttggtc gcgtcggtgt
    69061 cgtaaccagg gattatctga tcgtaagcga ctcccagaat ctcttcggtt tcaatccagc
    69121 gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag cgcgtgttgt
    69181 ggcgtttgaa ttcccacggg ttaccatatt ccagccagta gtcaggcgac tctttctggc
    69241 taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg taaccgcgcc
    69301 ccggcaaccc taacgtcgcc agagaatcga ggaagcaagc cgccagacgt cccaggccac
    69361 cgttaccgag gcccgggtca ttttcttcat caatcagctc ttcgagattt aaccccatcg
    69421 cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg ttggagagcg
    69481 tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct tgcgacaact
    69541 gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca aataacgttg
    69601 cgttcagcca ttcatgttta ttggcgacga ccgggtcctt tccaatcgta aacatcagct
    69661 tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa tatgtaaacg
    69721 gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc tcacggtacg
    69781 acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc acaaaccgcc
    69841 acagtgaagg acgggaccac agtacaaaag cacgtcgaat agcccgtaac agcgaccagg
    69901 cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc tcaagagaac
    69961 agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc gtaccgtact
    70021 tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc agaatgacgt
    70081 ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga acgcccactt
    70141 ggccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga tcgcccgcgc
    70201 cgagtagcgc cagctgcccg ccctgctcca gaaggcccgg taaggcttcc agcaccaggt
    70261 cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact ttatcgtcaa
    70321 ccttaagccc cattgcgatt tgtaactggc gcttattttc cgctttatct tccaacgtat
    70381 cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt ttctcgtcca
    70441 cgccgttcag tacgccggaa agacgccctt cacggtgacg ctgttgcaac agaccttcca
    70501 taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg accgccgtaa
    70561 tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg aattccagcc
    70621 catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt gcataaaaca
    70681 tgccttgata ggccaggttg tgcacagtaa acaccgactt cgccggacgc ccgcgcgccg
    70741 ccagatacgc aggcgcaagg cctgcatgcc agtcgtgcgc atgcaccaca tcaggacgcc
    70801 agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc gcaaaacgca
    70861 atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc ggacgatcat
    70921 agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg aacaacagcg
    70981 tgatatgccc ggcaaaggta tcacgacggg atactacctg cgcatcggtc acgccacggc
    71041 gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg atttgtgctg
    71101 cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg aacatctctg
    71161 aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc gtagcatttc
    71221 gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat cttcctctgc
    71281 gttttcacca atcaccatgc cttccggaat aacacaagca cgatcgatga cgcagcggcg
    71341 cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat caatgttgca
    71401 gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg aaccggagat
    71461 cacacaaccg ccggaaacca gtgagttaag ggtcatcccg tggctaccgg agcgatcctg
    71521 cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat tgcgatcgta
    71581 catatccagc tccggcacca cagaggccag atcgaggttc gctttccagt aagcttccag
    71641 cgtacccaca tcgcgccagt acggctcggc atccggatcg gattgtacgc aagagagcgg
    71701 gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat ctttgccaaa
    71761 gtcgtggctg gagttctcat cgcgatcgtc ttcttccagc agttcataca gatagtcggc
    71821 gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg gcattgacgg
    71881 tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa ccgccataac
    71941 gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc aacgtgcgcc
    72001 tttttcgacg tgatcgataa gcatacgcga gtagtcttgc ttgtagatat ggtcgcccgc
    72061 caggatcacc acgtattccg ctttataacg gcgaataata tcgaggtttt gggtgaccgc
    72121 atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg gcagcagatc
    72181 gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt gctgcaccag
    72241 agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccggagt tgatgcagtt
    72301 agacagcgca aagtcgataa tgcggaactt accgccgaag tgtacggccg gttttgctcg
    72361 cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca gggcaacaga
    72421 tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac taaccatgac
    72481 taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc ctgccagaca
    72541 gccgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca ctccccagca
    72601 ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa gcgatccgag
    72661 agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa aggttgagca
    72721 tatcgattta gccaacggac attgccgtcg ccttcttccc accagcgatt ctccaccaaa
    72781 gcgggaatac gcttgcgcag atggattaac gcggcggtaa atgcggttaa accactgctt
    72841 gcctgcgacc agtccaacca ggttaattgg ttatcctgac agtaggcatt gttattgcca
    72901 tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtgccctg ggagagcaac
    72961 aacgttgtta acagggcatg aatgctgtcg cgccgccgtt caaccagatc aagagtaccg
    73021 cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc gcgattttct
    73081 tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag cgtaaaaccg
    73141 tcatgtgcgg tgacgagatt aatcgcggca ctcggcagac gaccattacg tttaaaaaca
    73201 tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata atgcagccag
    73261 aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg cgggaaattt
    73321 cccacctgat aaccaccagg agcgatatcc cacggttcag caattaactt cacctgcgag
    73381 agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa ctctggcgta
    73441 cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt ttctacccaa
    73501 taacgcaggc aggcgctggc ataatccacc accgccgggt gactcaaatt gagcgtgttg
    73561 ccacaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata gctacggttg
    73621 tcgatcccac gcagcgagaa taacgggccg tcgaggtcca gttccgcact atggttgagc
    73681 acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc atcgcgaaac
    73741 tcatgcagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa catcgccacc
    73801 gggttgtaac cccagtagtt acttagcccc atgcgttgca gacgtggttc actggcaaac
    73861 tgcgccactg gcagcagttc cagcgcggta atgcccaact gtttcaaata gttgatcatc
    73921 accggatgcc cgagggcttt ataagtgcca cggatctcga ccgggatctc cgggtgcagg
    73981 tacgttaatc ctttaacatg ggcttcataa atgatggtgc tgccccacgg cgtgcgcggc
    74041 ggggcatcat cttcccagtc atagtgatca accaccacta cgcatttcgg cgcaatggcg
    74101 gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt atctttaaac
    74161 tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt aaagcgatgc
    74221 ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc cgggcgcgca
    74281 tccggcagat aaccgtgcca aatgtcgcca ctgtgccctg gcaagtcata gcgatgttcc
    74341 tggccattgg tgtcaaagac acacagctct acccgctcgg catgagcgga gaaaagtgtg
    74401 aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggag cgggtttgcc aatggcgagt
    74461 tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagc gtcaggctta
    74521 gtgaatgctg acgaccatgg ctggcaatct catcgctgtg taccgtgccg ccattgcctg
    74581 cattactgcc gtgatagtgc atggaatcgg tattgaggat ctcacgccat ttgcctggct
    74641 gattaatgcc gaagcgataa tcatgacgcg gcaccggcgt aaagttactg gcaacgatga
    74701 tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct ttgtcatcca
    74761 ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcatcgct ttatggtggc
    74821 ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag ttatcgccgc
    74881 cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc tgggcaaatt
    74941 cgttccccat gaacagcagt ttcttgcccg gaaatgccca catccagcca tagtaagcgc
    75001 gcaggttagc gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc gattttttac
    75061 cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag ttgtagagca
    75121 tcccgaaggt cagtttatcg tgatgatact gacgataaat cgggtcgagc ttcatgtagt
    75181 ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga ccgcccatat
    75241 cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc accgcaccgg
    75301 aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct tcaagattct
    75361 cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag tcacgataaa
    75421 tcatcgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca atccagtaaa
    75481 gcgcgttacc gacgaggaag ttactgactt cacggcgacc atagttgtaa atcagcgtgt
    75541 tccagtcctg atgatagcct tcgcgcggat cgctgtgttc atacaagttc gtaccatcaa
    75601 attcggcaag cgcaaagtca tcagtcggga agtggcctgg cacccagtcg agaatcacgt
    75661 tcagaccagc tgcgtgtgca gcatcaatga aataacggaa gtcgtcgcga gtaccaaaac
    75721 ggcgggttgg tgcatacagg ccggttggct gataacccca actgccatcg aagggatgct
    75781 cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa ggcaccagtt
    75841 gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga cgccaggagc
    75901 ccaggtgaac ttcatatata gagattggcg catcaaactg attcgctttt ttgcgctctt
    75961 cagtctgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc gggcgcattt
    76021 gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca tcaatcatct
    76081 cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag atgccgctct
    76141 ctttacgcag gcgcatcggg tgacggcgac cgtcccagta gttgaattgc ccaaccaccg
    76201 agacccgacg ggcgtttgga gcccagacag agaaacgcgt acctgtgacg ccatccatag
    76261 tatctgcatg cgcgcctaaa gtttcatacg ggcgcaggtg agtaccttca gataatagcc
    76321 aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatcaatc aggttttgct
    76381 gcccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc ggaatgacgc
    76441 cactaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc ccggttttcg
    76501 gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc agtcccgcgg
    76561 tggttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca attagcgcgt
    76621 taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat tgtgtcaccc
    76681 catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac ggcgcagcac
    76741 tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac tcatgtattc
    76801 cgccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat gaaacatctg
    76861 cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt taatgaataa
    76921 agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc acagcagctg
    76981 gtcgcccacg gtaaaggctg acaggaactc tggtcccata ttcagcttac gcaggcggcc
    77041 taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca tagtgatttc
    77101 ccgatcgttc ggaacgactt tcgcccacgg attgtgcgca gccagcagtt cttccacggt
    77161 cggaatcgac acatcttttt tcaatttaat agtgaatgcc tggctgtggc agcgcaatgc
    77221 cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga ggatcttgtt
    77281 ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcgagct gtttgtcgat
    77341 ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca gctcaccgct
    77401 acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg gggtcgcgag
    77461 ttcatctgct acatggccat acagatggcc catctgggtt aataactcac gcatatgtcg
    77521 cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa caagatcatt
    77581 ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac cgccaacaaa
    77641 agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg ggtcaagaat
    77701 aatgatggcg tcatctttca tgcgcagaga cgatgctgcg tcaatccagt aaccctgcca
    77761 tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct gacaggtcac
    77821 aatgatatcg agggccttta gcgcctccag atcaaaggca tcctgaagtg tgccagtggt
    77881 tccgccaaaa gacggcgcag cctgaccaag ctgagaagta gaaaagaaga cagggcgaat
    77941 ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga ccataccgcg
    78001 ccagccgata aaaccaacat ttttcataag cgttttttcc tgcaaagatg tgtgctgtat
    78061 aaatgtgccg gtctcctctt ggcacatctt tcaccataca aaaagcagcc aaagtcgcaa
    78121 gtgaaattaa tcaatgatag cgaagccatc agtaatgcga cttatcctgc tttgttagca
    78181 cgcagaaagt ccgcggcaat tatcagggaa tttgagttat gaatgaaatc atttctgcag
    78241 cagttttatt gatcctgatt atggatccgc tcggaaacct acctattttc atgtccgtac
    78301 tgaaacatac tgaaccgaaa agacggcggg caatcatggt gcgagagttg cttattgctc
    78361 tcctggtgat gctggtgttc ctgtttgcgg gtgagaaaat tctggcattt ctcagcctga
    78421 gagcagaaac cgtctccatt tctggcggca tcattctgtt tctaattgcc attaagatga
    78481 ttttccccag cgcttcagga aatagcagcg ggcttccggc aggtgaagag ccatttatcg
    78541 tgccgctggc aattccgctg gtcgccgggc caactattct cgccacgctg atgttgttgt
    78601 ctcatcagta cccgaatcag atgggacatc tggtgattgc tctgctgctg gcctggggcg
    78661 gcacctttgt catcctgcta cagtcttcgc tatttttacg tctgctgggc gagaaagggg
    78721 tgaacgcact tgaacgcctg atgggattga ttctggtgat gatggcaacc cagatgttcc
    78781 tcgacggcat tcgaatgtgg atgaaggggt aaggttcaaa caggtgctgc acacaaccag
    78841 acgcggcacc tgttttatta cctgaactga taatgacctt cgcccaatcg gataaccctt
    78901 ccctcttctt ctgcgcggtt cagccacaca ctaatttgtt cgatggttaa accagtacaa
    78961 gacgccagcc tttcaatact tatcgattcc tttgcaagga tcgccagttc cgccacaaag
    79021 agttgataaa aatcgacagg cgtatacgcg ggcattactg cgtaatttgc gtcatcttca
    79081 tgcagcgaaa acagttctgg ttgagcgtta cggagtgctc tgtcatacat cagcccagga
    79141 acgtctggca gtctgatcgt cacattctca gcctgttcag cactccatga cgccccattt
    79201 tctactaatc gtgaattggc tgattgcata tcctgatttg gtttaacctg cacaggcagc
    79261 cactgatgtt ttaatgcctc catcgccccg gttatcgttc caccggtcat tcccgcacgt
    79321 acaaccattg cgctttcagc aaggcaataa atatatttat ttcgcgccat cgcattgccg
    79381 acggtgaaac gtacctctgg gtaaaacggc gaaatcaaca ccaggttgcc tgctataagc
    79441 ccttcacgcc atttcactaa cgtactcgtt tttaacaagc tatcagctaa tacgccaaca
    79501 gcagttcccc cggcctccag tgccgacgcc attgcacatt catcaatacc tcgcgcacca
    79561 ccagagataa cgcaaatccc ctgttgggcc agtttcgcgg ccagttgttg ggtatagcgc
    79621 aaatcgtcag tcggagcatc tcgcgagcca acaatcgcca taccttccgc ttgcagtaat
    79681 gctttattcc cgcagccaaa taaaacggga ggtgcatccg ttcgcaatcg gtttttcaag
    79741 cgaacgggat aatcagcatc tccgcgggtt aagatccata aaccggcgcg ttgccactta
    79801 tctaccgcca acgccagact atgaccacgc gccagcaagc caagaatacg ctcctgagaa
    79861 atacgcggat cgctccagcc tgtaagtttc tcttgcggtt gcggcaccag aagctcggcg
    79921 ggactgatac gttggtgctt cagccataat gcaaacttcc cccattcact attactaaga
    79981 ggtttatact cactcgccgc cgcgcgagaa aaatcgctgg ttagcaggag agttgcttgt
    80041 gcattggctg aaagattcat caatttttta ccgaggtaga agcaagggca agaggataaa
    80101 ccgtcgggca acctgcctgg cgtaacagtg ctgtcagaac tgtcagcgtc catgcagaat
    80161 ccacgatatc gtcaaccagc aacgccggac caggcatcaa aggaggggta atcacaaacg
    80221 ccccgtcgag attttgacac tggtggaaac ggttttgctg cattttctgc ggtggattgt
    80281 ccacgacttt ttcaatggca tcaataaaag gtaagccaag tttcgccgcc agtcgccggg
    80341 caaaatcagg aaccaggtcg aggtgcttta atgaaggaac gcagcatacc caggttggtt
    80401 caggatgcgg attccagcgt tggcgaacca tttccgcaca tgcctccacc agttcatcac
    80461 tgaatcgccc cgtttttttc tcctgtgcta cctgctttcc ccacccggaa tctccccaac
    80521 gagaaaggat tcgccccgta gatccttgta aactcgcagg caagttccct ttaaatccgt
    80581 attgcgtaaa agccccagct gccacctgtt tattgagatt taatggcagg tcagcgtgtt
    80641 taataaataa gtttgccgca tggagtaagc cgctatcaat gtcgggcgac aacaacggat
    80701 gctgaagaca actgctacat ttaccacaat gttcggcacc tggctcatcg agtgcgcggc
    80761 gcagaaattg cattttgcac tccttggtcg tgatatagct ttcaacgtct gccagctcac
    80821 tttttctctg attcatcaaa tggttaattc gttcatggtc aggagaaaaa ctgacaattg
    80881 ttctgcgcca taatttctcg gtatacacca caggcgatgg attttcagct accaataatt
    80941 tcagtgcttt ttctatttgc ccataacgga gattcgtccg ttgttcaatg cctcgtaagg
    81001 taagaccgtc attcacgcta agtacgttga gtatttcatg aatttgcgcc tccgcaggaa
    81061 aggcgctttc acggaagaat ttatgaatag cacggtcttc actgccgcaa agcagtatgc
    81121 caacagcaga atcgataccg cgaccggcac gccccacctg ttggtagtaa ccaactatcg
    81181 aacctggcat ctggtaatga ataacaaacc ccaaatccgg tttgtcaaag cccattccca
    81241 gcgccgttgt cgcaaccagc actttaattt tatttgccag caacgcctgc tccagatatt
    81301 ccctggcagt attagaatct tccgcgcctt cgcaagtcac gccgctgtag taagcaaatg
    81361 cactgatacc atttttcctt aaccactcgg cgacaagttc ggcatcgcga gtagtcaggg
    81421 tataaacaat tcccgatttg gaaaactgag ggataaccgt tgctaaccat gccagacggg
    81481 atgactgttc acctaagact aaggcatcaa gcgccaaact ttcgcgagcc agcgttccac
    81541 gctgaatcac aatgtcaccc aattgctgac ggatatcctc aacgacacgg ttattcgctg
    81601 tcgcggttgt acccagaaca ggggtattcg caggtagttg gcgcaaaata tctaatatac
    81661 gtcggtaatc cgggcgaaaa tcgtggcccc aatcagagat gcagtgcgcc tcgtcgacca
    81721 caagcaagcc aatacgatcg gctataggat ataaaaccgt ttcgataaag tcctggtttg
    81781 ccaaacgttc cggggaaacc agcaagcaat cgacctcacc ttgcagcaac ttatcgctaa
    81841 tgcgctgcca ttcttctctg tttgtagaat tcaacgtttc agccgtaata cctaaccgtt
    81901 cagcggcggc aacctggtta cgcatcaggg caagcaaagg agagatgata attgtcgggc
    81961 cagcgccgcg atcgcggaag attttactgg cgatgaagta cacagcactt ttcccccagc
    82021 cagtgcgttg gacaaccagc agttttcgcc gttggttcac caccgcgtct atggcttccc
    82081 attgcccttc cctgaaagta gcggtggtat ttcctaacat ccgctgtaaa agcaattcag
    82141 ctgcgtgctt ttccatcttt atctcctgcg atctttaggc agtgattaga acggattatt
    82201 tgcaggtaaa tttgcgagag agatcaaaaa gatgaagaga ggagaaaaga gaaatgaatt
    82261 actgcgagca agctcgcagt aatttgtcgc agtaattaac tcaacagctg gaacgcaatc
    82321 atcccaacga tggcaccgac agtgccgagg atggtttcca tcatggtcca ggttttcagc
    82381 gtttcggctt cggtcgcgcc ggtaaattta ccgaacaacc agaaaccagc gtcgttaacg
    82441 tggctgacaa caatcgaacc accggcgata caaatcgaca gcgccgccat ttgcgcaccg
    82501 gagtagttca gttgttcaat aaccggcatc accagtccca ccgccgttaa acaggcaacg
    82561 gttgcagaac cctgaatgat gcgcactgca gcggccagca cgaagcaggt gatggcaatc
    82621 ggcaggccca tgccggttaa cgcttcaccc agcgccggac ctacgccaga gtcaaccagc
    82681 acctgtttga acacgccgcc tgcaccaatc accagcagaa tgatccccgc cggttgcagc
    82741 gcgtgaccgc aaatctccat cactttgtct tttggcatgc cctgacgcat tgccaggcca
    82801 taaatcgcta ccagacaagc aaccagaatc gcggtaaacg gatgaccaat aaactcgaac
    82861 cattcgtaag cggtagagcc ttccggcaca aaacgcgcgg caatggtttt cagccccacc
    82921 agcaccagcg gcagcaggat cagcgacagg ctgaatccga aggacggcat tttgccttcg
    82981 ccgagatgcg gctcgctgat gtcgtcagga atatgcagct caacgtaacg gctgatgaag
    83041 ttaccccaca gcggcccggc aataatcatt cccggaattg ccgcacacag gccaatcagg
    83101 atcatccagc caaaatcggc gttcatctgc gatgccagca gcattggcgc tggccctggc
    83161 accaggaacg ccgccgctgc cgccacgcct gcgaataatg ggattaccag cttcaccagg
    83221 ttcgtaccgg tgtggcgcgc cattgagaaa gcaacgctaa tcagcagaac aatcgccact
    83281 tcaaagaaca gcggtagcgc acagaccagc cccgcaaggc cgatggcata atgcgcgcgg
    83341 ctgtgaccga aggatttaag cattttgacg gcaatctgat cgactgcgcc ggtttcatgt
    83401 aagatcttgc caaacatagc tcccagggcg acaaccaccg ccaggaagcc gagggtgcct
    83461 cccatccctt tttccatcgt cgctgcgatt ttatcgagcg gcataccgga aaaaaggcca
    83521 gcccccatgg acaccaccat taaagccagg aaagcgtgca tacgcgcctt catgactaaa
    83581 aacagcagca gtaaaacaga ccctactgct gttaaaacaa gcgttaatgt agtcactact
    83641 tatttgcctt ttttaataac ctcaatggtg cttgccacaa caccttccag cggttgatcg
    83701 atatccacca ccagtacatc ggtttcgtcc gcacccggct cctgcagcgt ttcaaactgc
    83761 gtcaccaaca tttgggtttt aaagaaatgg cctttgcgcg ctttcaggcg gctttcaatc
    83821 acatcaaaat cgcctttcag gtagatgaaa gagagattcg gattaccttc acgcagcaag
    83881 tcgcgatagt gttttttcaa tgcagaacag acgatcagcg acactttatt agtgcgctgc
    83941 atagcaaacg cggcgtcgtt cagcgcctgc aaccacggtt tgcgatcgtc gtcattcagc
    84001 ggttcgccag acgccatttt ttcgatattg cgccgtggat ggaggaaatc gccatcaaga
    84061 aacgcggcat gaagttgatg cgccacttca cttgcgaccg cagatttgcc gctgcccgat
    84121 acgcccatca agacgtaaat gtggtgatca tggttagtcg tgctcaaagt ggtgccccca
    84181 caatacaaga attaaaaatg ttacgggtaa ctgttatcgg taacattctc cggccggaca
    84241 atatcagaag caatatccat ccgtgcctta agtgtataag tgtgagctac ttcaaatttg
    84301 tgggcttaaa tagatccgcc cggtgacaag gtgaaaccta aatctaacat tttcggtgtc
    84361 acagattcgc cacgaatacg cgccagaagg cgttcagcgc caatactgcc catccgctca
    84421 cgcggcgtca gcacgctcgc aagtcgtggc tccatcacct gaccaatgtc atgaccgtgg
    84481 aaaccggcaa tcgccatatc gtcaggaact tttaacccca gacgctgaca ttcaaacgcc
    84541 gcgccgaccg ccagatcatc attagtacag aacacgccat ccagctgcgg atattcccgc
    84601 cgcgcctggc gaatcagttc aataccggaa gagtaagaag aagattgctc aaccatcacg
    84661 ctatacggca ccaggcctgc atcccgcatc gcctgttcgt atcccttctg tttgatgata
    84721 gtacgttcgt cgagacgtgc gccgagatag gcaatatggc gatgcccgcg agcaataatg
    84781 gcggcggtca tctggcgtgc cgcttcaaag ttatcaaaac cgacggcaat atcgaggcat
    84841 ggcgacttgc tgtccatcag ttccaccacg ggaatccccg ccacttcaat catctttaag
    84901 gtgcgcggcg tgtgggtacg ttcggtgagg ataagtccgt cgatattcca tgagagcatt
    84961 gattcgagac gttcttgctc catttccggt ttataaccgt agtgggccag catggtctga
    85021 taaccgtgcg cgtcggtgac gctttcgatt ccgcgtaata cttccgcgaa aacctggttg
    85081 gtgagagaag gtaacaggac gccaatcgcc cggctggtgg cgttagagag gatatcgggc
    85141 gcacgattgg gaatatagcc cagttcatca agagccgcgg caatcttgcc gcgtagagcg
    85201 acggaaacct gctccgggtt gcgtaaaaaa cggctgaccg tcattttggt cacgcctaca
    85261 cggtcagcca catcctgaag tacgggtctt ttctttttca tcgtcctgaa ggtacaaaag
    85321 agatagattc tcttagttta acacggacgc atcagaacct tcccggggaa aacagtgctt
    85381 gtttacaatt attagatata cactaaatca attgcatcgc attgtgctaa cgacgcggca
    85441 aaaccccgga agcgtacatc agcacgcgac cggagcgagc gaggaagcct gcaaaagggc
    85501 aggcttcaaa gatgccgttg agttttaaac cggcggcaga tcgaacagta acacttcgct
    85561 gtcgctatcc gcatggatgg agattgcctg ctcatcccag attgccagac catcgctggt
    85621 cgaggctttc acgccgttaa tggtgacatt gcctttcacc acctggatcc agacgcggcg
    85681 ttcagcggca atctgatgca ccgactgctc atctttcagc aacgcccagc ggtacagttc
    85741 catatcctga tgcactttca atgaaccatc tcgcgcatct ggcgaaagca ccagttgttt
    85801 gccctgcacg gcatcgaagc gacgctgttc ataacgcggc gtaataccgt tttcttcagg
    85861 catgatccag atctgataca gatgcagacg ctcggtgctg cttgggttgt actctgagtg
    85921 acgaataccc gtacccgcac tcataatctg gaactcgccc gccggaacct gctctttatt
    85981 gcccatgctg tcctggtgct caacagtacc ttccagcacg taggtcaaaa tttccatatc
    86041 tttatgcggg tgagtgccga agccctgccc tgcttcaatc acgtcgtcgt taatcacgcg
    86101 cagcgcggag aagcccataa agttcggatc gtaatagttg gcaaaagaga aagtatgcca
    86161 ggagtccagc cagccatgat ttgcatgacc acgttcattt gctttgcgta agtagatcat
    86221 ttgtattcgc cccctgaatg atttcgatgg gcttagtgtg gacccgatct gcctgggatg
    86281 atagagggtg aaaattgacc cctctgttca aaaaaattga actatttgag gggccatcaa
    86341 acttacttcg cgagagtcac ggtggaggga gaggcttgct cgaatccgcg ttcaaggatt
    86401 tccagattcg taagaacttc agattccttg acgtaatttg gcgcaccgtg ggtgatggtt
    86461 tgatacaacg catcataaac gcgcccgtaa tcgcccatct ccggcttcat ctcttctctg
    86521 accgtcacgc cctcgtcatt gacatactcc agcacaccga ccgaatcatc cgctgcgaat
    86581 cccggttcgc ccggcataat attagccttc aggctggttt cctgctggtc gataccgtat
    86641 ttaataaacg aacctttctt accgtgaacg ataaatttcg gataatcgat tttcaccaga
    86701 tggctggttt tgacgatggc ttttagatcg ccataaaaca gttgcgcttc gaaagtatcg
    86761 tcaggattgg ctttattgcg caggctgcgg atgtcataag cgacgtgatc cgggcgaccg
    86821 aacagagaaa taatctggtc catcgtatgc acaccaaggc catagaacgc gccatcctgc
    86881 ggcagcccag gtttggtttc tgccaccggg cggtaatagt caaaatggct ttccacttcg
    86941 acaatctctc ccagcttgcc gctttcaatc gcttttttcg ctgtcaggaa gcaggaatca
    87001 aagcgacgat tctgatatgg cgtgacggtc agccctttgc ttttcgccag tgcaaacagc
    87061 tctttcgcct gcgcaagtgt cggagtgaac ggtttttcga ccagcacatt tttcccggct
    87121 tccagcgcgc gtttcgcgta ctcgaaatgg ctgtccgcgt gggtgcagac cacaaccagc
    87181 ttaacatcgg gatcgtttag cacttcgtcg agatcgctgg tgaaatggat atgagagtaa
    87241 atgggagcct gttcttccgg cttcgcatgg cgacgaaaaa tatgcgcgac atgccagcta
    87301 tccttgcggt taagtacata cggcagatgg taacgggtgg tgcttttgcc gaagccaata
    87361 aaggcgcagt tgatgaccat gatttcgtcc tttttaaggt ggttatttac accttagcgc
    87421 aaagcggacg tggttcctac tggagtgcgc ataacgaaca caagcacttc ccgaggataa
    87481 attgagaacg aaagatcaaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc
    87541 aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgagacaat
    87601 aataatcatt ctcattcgca cttgtccaac actttttgca aaaaaaatgc atttgactcg
    87661 cattcgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    87721 gagatagtga tacgccatgc tgaaacgcgc gattacgagg ccatcaggca gattcacgcc
    87781 cagccggagg tgtattgcaa cacattacag gtgcctcatc cttccgatca tatgtggcag
    87841 gagcgactcg ccgatcgtcc cggcatcaaa cagttagttg cctgtattga tggaaccgtc
    87901 gtgggtcacc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    87961 ggtatttgtg tcgactcccg ttggaagaat cgcggcgtcg ccagcgccct tatgcgagag
    88021 atgattgaaa tgtgcgacaa ctggttgcgg gtagatcgta tcgagctaac ggtgttcgtc
    88081 gataacgcgc cagcaattaa ggtctataaa aaatacggct ttgaaattga agggactggt
    88141 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaag
    88201 taaagaatat tctcaaattg cctgttacca atgtgaataa ttagttttgt tcatctaatt
    88261 tgagtttttc cttatattac caccatcatt ttgaaataat ttgtaacatc
//
LOCUS       DAZFKF010000006        87559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFKF010000006 DAZFKF010000000
VERSION     DAZFKF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 87559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.006"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(32..1891)
                     /gene="gss"
                     /locus_tag="ACHKA1_000511"
     CDS             complement(32..1891)
                     /gene="gss"
                     /locus_tag="ACHKA1_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO5678215.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2096..2962
                     /gene="yghU"
                     /locus_tag="ACHKA1_000512"
     CDS             2096..2962
                     /gene="yghU"
                     /locus_tag="ACHKA1_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO5678216.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3085..3333)
                     /gene="hybG"
                     /locus_tag="ACHKA1_000513"
     CDS             complement(3085..3333)
                     /gene="hybG"
                     /locus_tag="ACHKA1_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO5678217.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3346..3687)
                     /gene="hypA"
                     /locus_tag="ACHKA1_000514"
     CDS             complement(3346..3687)
                     /gene="hypA"
                     /locus_tag="ACHKA1_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO5678218.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIIYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3680..4168)
                     /gene="hybE"
                     /locus_tag="ACHKA1_000515"
     CDS             complement(3680..4168)
                     /gene="hybE"
                     /locus_tag="ACHKA1_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO5678219.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPLMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4161..4655)
                     /gene="hybD"
                     /locus_tag="ACHKA1_000516"
     CDS             complement(4161..4655)
                     /gene="hybD"
                     /locus_tag="ACHKA1_000516"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO5678220.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4655..6358)
                     /gene="hybC"
                     /locus_tag="ACHKA1_000517"
     CDS             complement(4655..6358)
                     /gene="hybC"
                     /locus_tag="ACHKA1_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO5678221.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6355..7533)
                     /gene="hybB"
                     /locus_tag="ACHKA1_000518"
     CDS             complement(6355..7533)
                     /gene="hybB"
                     /locus_tag="ACHKA1_000518"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO5678222.1"
                     /translation="MSHDPKPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7523..8509)
                     /gene="hybA"
                     /locus_tag="ACHKA1_000519"
     CDS             complement(7523..8509)
                     /gene="hybA"
                     /locus_tag="ACHKA1_000519"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO5678223.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8512..9630)
                     /gene="hybO"
                     /locus_tag="ACHKA1_000520"
     CDS             complement(8512..9630)
                     /gene="hybO"
                     /locus_tag="ACHKA1_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO5678224.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVIGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9819..10106)
                     /gene="yghW"
                     /locus_tag="ACHKA1_000521"
     CDS             complement(9819..10106)
                     /gene="yghW"
                     /locus_tag="ACHKA1_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO5678225.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10225..11112)
                     /gene="yghX"
                     /locus_tag="ACHKA1_000522"
     CDS             complement(10225..11112)
                     /gene="yghX"
                     /locus_tag="ACHKA1_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO5678226.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11269..12309
                     /gene="gpr"
                     /locus_tag="ACHKA1_000523"
     CDS             11269..12309
                     /gene="gpr"
                     /locus_tag="ACHKA1_000523"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO5678227.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLVGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12349..12843)
                     /gene="yqhA"
                     /locus_tag="ACHKA1_000524"
     CDS             complement(12349..12843)
                     /gene="yqhA"
                     /locus_tag="ACHKA1_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO5678228.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13034..13918
                     /gene="yghA"
                     /locus_tag="ACHKA1_000525"
     CDS             13034..13918
                     /gene="yghA"
                     /locus_tag="ACHKA1_000525"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO5678229.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIHLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVS
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14190..14615)
                     /gene="exbD"
                     /locus_tag="ACHKA1_000526"
     CDS             complement(14190..14615)
                     /gene="exbD"
                     /locus_tag="ACHKA1_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO5678230.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14622..15356)
                     /gene="exbB"
                     /locus_tag="ACHKA1_000527"
     CDS             complement(14622..15356)
                     /gene="exbB"
                     /locus_tag="ACHKA1_000527"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO5678231.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15608..16795
                     /gene="metC"
                     /locus_tag="ACHKA1_000528"
     CDS             15608..16795
                     /gene="metC"
                     /locus_tag="ACHKA1_000528"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO5678232.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16935..17594
                     /gene="yghB"
                     /locus_tag="ACHKA1_000529"
     CDS             16935..17594
                     /gene="yghB"
                     /locus_tag="ACHKA1_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO5678233.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17634..18533)
                     /gene="yqhC"
                     /locus_tag="ACHKA1_000530"
     CDS             complement(17634..18533)
                     /gene="yqhC"
                     /locus_tag="ACHKA1_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO5678234.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18727..19890
                     /gene="yqhD"
                     /locus_tag="ACHKA1_000531"
     CDS             18727..19890
                     /gene="yqhD"
                     /locus_tag="ACHKA1_000531"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO5678235.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19995..20822
                     /gene="dkgA"
                     /locus_tag="ACHKA1_000532"
     CDS             19995..20822
                     /gene="dkgA"
                     /locus_tag="ACHKA1_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO5678236.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21022..21948
                     /gene="yqhG"
                     /locus_tag="ACHKA1_000533"
     CDS             21022..21948
                     /gene="yqhG"
                     /locus_tag="ACHKA1_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO5678237.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSVRIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITETD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRIDEIRVLEKTADSARIHIRFTLTNGNNEEQELVLQR
                     RDGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21997..22254
                     /gene="yqhH"
                     /locus_tag="ACHKA1_000534"
     CDS             21997..22254
                     /gene="yqhH"
                     /locus_tag="ACHKA1_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO5678238.1"
                     /translation="MKTIFTVGAVVLATCVLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22297..24516)
                     /gene="ygiQ"
                     /locus_tag="ACHKA1_000535"
     CDS             complement(22297..24516)
                     /gene="ygiQ"
                     /locus_tag="ACHKA1_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO5678239.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGGRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24627..26039)
                     /gene="ftsP"
                     /locus_tag="ACHKA1_000536"
     CDS             complement(24627..26039)
                     /gene="ftsP"
                     /locus_tag="ACHKA1_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO5678240.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26114..26851)
                     /gene="plsC"
                     /locus_tag="ACHKA1_000537"
                     /gene_synonym="parF"
     CDS             complement(26114..26851)
                     /gene="plsC"
                     /locus_tag="ACHKA1_000537"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO5678241.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27085..29343)
                     /gene="parC"
                     /locus_tag="ACHKA1_000538"
     CDS             complement(27085..29343)
                     /gene="parC"
                     /locus_tag="ACHKA1_000538"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO5678242.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMLRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29481..31088)
                     /gene="ygiS"
                     /locus_tag="ACHKA1_000539"
     CDS             complement(29481..31088)
                     /gene="ygiS"
                     /locus_tag="ACHKA1_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5678243.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTILQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31197..31679)
                     /gene="ygiV"
                     /locus_tag="ACHKA1_000540"
     CDS             complement(31197..31679)
                     /gene="ygiV"
                     /locus_tag="ACHKA1_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO5678244.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(31732..32124)
                     /gene="ygiW"
                     /locus_tag="ACHKA1_000541"
     CDS             complement(31732..32124)
                     /gene="ygiW"
                     /locus_tag="ACHKA1_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO5678245.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32276..32935
                     /gene="qseB"
                     /locus_tag="ACHKA1_000542"
     CDS             32276..32935
                     /gene="qseB"
                     /locus_tag="ACHKA1_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO5678246.1"
                     /translation="MRVLLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALGERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32932..34281
                     /gene="qseC"
                     /locus_tag="ACHKA1_000543"
     CDS             32932..34281
                     /gene="qseC"
                     /locus_tag="ACHKA1_000543"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO5678247.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFVRTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(34327..34659)
                     /gene="ygiZ"
                     /locus_tag="ACHKA1_000544"
     CDS             complement(34327..34659)
                     /gene="ygiZ"
                     /locus_tag="ACHKA1_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO5678248.1"
                     /translation="MLKHEIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLDSGDNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIIIILRKLFVREMFEFWLYVCSFVICLVCGWWLF
                     WGRFIFCY"
     gene            35017..35598
                     /gene="mdaB"
                     /locus_tag="ACHKA1_000545"
     CDS             35017..35598
                     /gene="mdaB"
                     /locus_tag="ACHKA1_000545"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO5678249.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35629..35943
                     /gene="ygiN"
                     /locus_tag="ACHKA1_000546"
     CDS             35629..35943
                     /gene="ygiN"
                     /locus_tag="ACHKA1_000546"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO5678250.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(35991..37883)
                     /gene="parE"
                     /locus_tag="ACHKA1_000547"
     CDS             complement(35991..37883)
                     /gene="parE"
                     /locus_tag="ACHKA1_000547"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO5678251.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(37912..38493)
                     /gene="yqiA"
                     /locus_tag="ACHKA1_000548"
     CDS             complement(37912..38493)
                     /gene="yqiA"
                     /locus_tag="ACHKA1_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO5678252.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(38493..39320)
                     /gene="cpdA"
                     /locus_tag="ACHKA1_000549"
     CDS             complement(38493..39320)
                     /gene="cpdA"
                     /locus_tag="ACHKA1_000549"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO5678253.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(39345..39767)
                     /gene="yqiB"
                     /locus_tag="ACHKA1_000550"
     CDS             complement(39345..39767)
                     /gene="yqiB"
                     /locus_tag="ACHKA1_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO5678254.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(39768..40397)
                     /gene="nudF"
                     /locus_tag="ACHKA1_000551"
     CDS             complement(39768..40397)
                     /gene="nudF"
                     /locus_tag="ACHKA1_000551"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO5678255.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWV"
     gene            40602..42083
                     /gene="tolC"
                     /locus_tag="ACHKA1_000552"
     CDS             40602..42083
                     /gene="tolC"
                     /locus_tag="ACHKA1_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO5678256.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            42231..42902
                     /gene="ygiB"
                     /locus_tag="ACHKA1_000553"
     CDS             42231..42902
                     /gene="ygiB"
                     /locus_tag="ACHKA1_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO5678257.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            42908..44068
                     /gene="ygiC"
                     /locus_tag="ACHKA1_000554"
     CDS             42908..44068
                     /gene="ygiC"
                     /locus_tag="ACHKA1_000554"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO5678258.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(44106..44894)
                     /gene="ygiD"
                     /locus_tag="ACHKA1_000555"
     CDS             complement(44106..44894)
                     /gene="ygiD"
                     /locus_tag="ACHKA1_000555"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO5678259.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            45037..45810
                     /gene="zupT"
                     /locus_tag="ACHKA1_000556"
     CDS             45037..45810
                     /gene="zupT"
                     /locus_tag="ACHKA1_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO5678260.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(45868..46038)
                     /gene="yqiD"
                     /locus_tag="ACHKA1_000557"
     CDS             complement(45868..46038)
                     /gene="yqiD"
                     /locus_tag="ACHKA1_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO5678261.1"
                     /translation="MFIAWYWIVLIVLVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(46300..46953)
                     /gene="ribB"
                     /locus_tag="ACHKA1_000558"
     CDS             complement(46300..46953)
                     /gene="ribB"
                     /locus_tag="ACHKA1_000558"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO5678262.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(47057..47205)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            47327..47626
                     /gene="ubiK"
                     /locus_tag="ACHKA1_000559"
     CDS             47327..47626
                     /gene="ubiK"
                     /locus_tag="ACHKA1_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO5678263.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(47661..47861)
                     /gene="glgS"
                     /locus_tag="ACHKA1_000560"
     CDS             complement(47661..47861)
                     /gene="glgS"
                     /locus_tag="ACHKA1_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO5678264.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            48130..48759
                     /gene="yqiJ"
                     /locus_tag="ACHKA1_000561"
     CDS             48130..48759
                     /gene="yqiJ"
                     /locus_tag="ACHKA1_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO5678265.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            48786..50447
                     /gene="yqiK"
                     /locus_tag="ACHKA1_000562"
     CDS             48786..50447
                     /gene="yqiK"
                     /locus_tag="ACHKA1_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO5678266.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            50686..50745
                     /locus_tag="ACHKA1_000563"
     CDS             50686..50745
                     /locus_tag="ACHKA1_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5678267.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            51062..51121
                     /gene="ibsE"
                     /locus_tag="ACHKA1_000564"
     CDS             51062..51121
                     /gene="ibsE"
                     /locus_tag="ACHKA1_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO5678268.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(51241..52674)
                     /gene="hldE"
                     /locus_tag="ACHKA1_000565"
     CDS             complement(51241..52674)
                     /gene="hldE"
                     /locus_tag="ACHKA1_000565"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO5678269.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQQARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(52722..55562)
                     /gene="glnE"
                     /locus_tag="ACHKA1_000566"
     CDS             complement(52722..55562)
                     /gene="glnE"
                     /locus_tag="ACHKA1_000566"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO5678270.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYVAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTVALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSENDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPTHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDVMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(55585..56886)
                     /gene="ygiF"
                     /locus_tag="ACHKA1_000567"
     CDS             complement(55585..56886)
                     /gene="ygiF"
                     /locus_tag="ACHKA1_000567"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO5678271.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAEEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            57128..57748
                     /gene="ygiM"
                     /locus_tag="ACHKA1_000568"
     CDS             57128..57748
                     /gene="ygiM"
                     /locus_tag="ACHKA1_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO5678272.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            57812..59050
                     /gene="cca"
                     /locus_tag="ACHKA1_000569"
     CDS             57812..59050
                     /gene="cca"
                     /locus_tag="ACHKA1_000569"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO5678273.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(59231..60052)
                     /gene="bacA"
                     /locus_tag="ACHKA1_000570"
     CDS             complement(59231..60052)
                     /gene="bacA"
                     /locus_tag="ACHKA1_000570"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO5678274.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(60143..60511)
                     /gene="folB"
                     /locus_tag="ACHKA1_000571"
     CDS             complement(60143..60511)
                     /gene="folB"
                     /locus_tag="ACHKA1_000571"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO5678275.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            60616..61233
                     /gene="plsY"
                     /locus_tag="ACHKA1_000572"
     CDS             60616..61233
                     /gene="plsY"
                     /locus_tag="ACHKA1_000572"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO5678276.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(61246..62178)
                     /gene="ttdR"
                     /locus_tag="ACHKA1_000573"
                     /gene_synonym="dan"
     CDS             complement(61246..62178)
                     /gene="ttdR"
                     /locus_tag="ACHKA1_000573"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO5678277.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            62385..63296
                     /gene="ttdA"
                     /locus_tag="ACHKA1_000574"
     CDS             62385..63296
                     /gene="ttdA"
                     /locus_tag="ACHKA1_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO5678278.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLSFENLSHTRSAL"
     gene            63293..63898
                     /gene="ttdB"
                     /locus_tag="ACHKA1_000575"
     CDS             63293..63898
                     /gene="ttdB"
                     /locus_tag="ACHKA1_000575"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO5678279.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            63947..65410
                     /gene="ttdT"
                     /locus_tag="ACHKA1_000576"
     CDS             63947..65410
                     /gene="ttdT"
                     /locus_tag="ACHKA1_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO5678280.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLMITGLLWMPVVLL"
     gene            complement(65453..66466)
                     /gene="tsaD"
                     /locus_tag="ACHKA1_000577"
     CDS             complement(65453..66466)
                     /gene="tsaD"
                     /locus_tag="ACHKA1_000577"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO5678281.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            66704..66919
                     /gene="rpsU"
                     /locus_tag="ACHKA1_000578"
     CDS             66704..66919
                     /gene="rpsU"
                     /locus_tag="ACHKA1_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO5678282.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            67030..68775
                     /gene="dnaG"
                     /locus_tag="ACHKA1_000579"
     CDS             67030..68775
                     /gene="dnaG"
                     /locus_tag="ACHKA1_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO5678283.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            68970..70811
                     /gene="rpoD"
                     /locus_tag="ACHKA1_000580"
     CDS             68970..70811
                     /gene="rpoD"
                     /locus_tag="ACHKA1_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO5678284.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(70890..71396)
                     /gene="mug"
                     /locus_tag="ACHKA1_000581"
     CDS             complement(70890..71396)
                     /gene="mug"
                     /locus_tag="ACHKA1_000581"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO5678285.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            71521..71596
                     /locus_tag="ACHKA1_000582"
     tRNA            71521..71596
                     /locus_tag="ACHKA1_000582"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:71554..71556,aa:Ile,seq:cat)
     gene            complement(71650..72414)
                     /gene="nfeF"
                     /locus_tag="ACHKA1_000583"
                     /gene_synonym="yqjH"
     CDS             complement(71650..72414)
                     /gene="nfeF"
                     /locus_tag="ACHKA1_000583"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO5678286.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEDGVASSWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            72702..73325
                     /gene="nfeR"
                     /locus_tag="ACHKA1_000584"
                     /gene_synonym="yqjI"
     CDS             72702..73325
                     /gene="nfeR"
                     /locus_tag="ACHKA1_000584"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO5678287.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(73432..74952)
                     /gene="aer"
                     /locus_tag="ACHKA1_000585"
     CDS             complement(73432..74952)
                     /gene="aer"
                     /locus_tag="ACHKA1_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO5678288.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSEMVKHRASRLEDAVTVLH"
     gene            75370..76749
                     /gene="ygjG"
                     /locus_tag="ACHKA1_000586"
     CDS             75370..76749
                     /gene="ygjG"
                     /locus_tag="ACHKA1_000586"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO5678289.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(76791..77123)
                     /gene="ygjH"
                     /locus_tag="ACHKA1_000587"
     CDS             complement(76791..77123)
                     /gene="ygjH"
                     /locus_tag="ACHKA1_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO5678290.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            77342..78325
                     /gene="ebgR"
                     /locus_tag="ACHKA1_000588"
     CDS             77342..78325
                     /gene="ebgR"
                     /locus_tag="ACHKA1_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO5678291.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            78509..81601
                     /gene="ebgA"
                     /locus_tag="ACHKA1_000589"
     CDS             78509..81601
                     /gene="ebgA"
                     /locus_tag="ACHKA1_000589"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO5678292.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHVEVRAEGETLTTRQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGKRYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            81598..82047
                     /gene="ebgC"
                     /locus_tag="ACHKA1_000590"
     CDS             81598..82047
                     /gene="ebgC"
                     /locus_tag="ACHKA1_000590"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO5678293.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            82110..83543
                     /gene="ygjI"
                     /locus_tag="ACHKA1_000591"
     CDS             82110..83543
                     /gene="ygjI"
                     /locus_tag="ACHKA1_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5678294.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            83677..84747
                     /gene="ygjJ"
                     /locus_tag="ACHKA1_000592"
     CDS             83677..84747
                     /gene="ygjJ"
                     /locus_tag="ACHKA1_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO5678295.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            84764..87115
                     /gene="ygjK"
                     /locus_tag="ACHKA1_000593"
     CDS             84764..87115
                     /gene="ygjK"
                     /locus_tag="ACHKA1_000593"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO5678296.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKSDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
BASE COUNT        21465 a        23527 c        22100 g        20467 t
ORIGIN      
        1 cataatcagt acggggttgc tacaacatgt attacttttt caccacaatt aacggttcaa
       61 tatcactctc ttttttaata accagtgatt catcaccgcg caaacacgtc ccaccgtagt
      121 tgccgccaac ggtgaaggta catacctgaa tgtatttacc gtccactttc ggcaaacacc
      181 acagttgctg atagatgttt ttctgctcgg caaatttacc actggttttg tccagcacct
      241 cttcatgatg gctgacgagg tcgatgttac tgccacagcg accggcgatc ggtttcactg
      301 cgtaacctgt tttcaccaat tcatcattaa cagtgaaatc ggtatccagc aggtaacgat
      361 ggtgcgggaa cagcgaccag aggatcggca gaatcgcttt gttgccgggg atcaccgtcc
      421 acagcggctc aaagaccagc acttccgggc gcagcaatac gtcgataaga cgcacttcat
      481 tttgcggatg accggtacgg atcggcaccg cagcaaactc acggtcgcta acttcacgaa
      541 tctgatcaaa cgcggtttcc cacgcccagg ttttccacac gcagttaacc agtcgccctt
      601 ccccatcaat cagttgcccg gcagcatccc agcccagttc atccagcccg cgcaagatac
      661 gcgtttcaaa gcccgcctgg tgcagcgcct gctccataaa ctgcgcgtga tagttttcct
      721 cgatatcttt gtcctgcatg atatggacaa acggacgtgc acgactgtgt ttccaggcac
      781 cagccagttc gttaatcagc ccttccgccg gattgaagcc gttgccttta tagccctgct
      841 ccgcccaacg ttcgaggatc aagcccgctt cggtatgaca tgaggcggag tcggcgttgt
      901 actcgtaaac cttcagacca cgctcatcca tgcagaaatc catacgaccg gtgatcatat
      961 ggtgacggcg acgctgccag gagagacgca aacgtggcca gaggattttc ggaatgtcga
     1021 acagcgccag caggttgtca tctttcagca ccttgtcggt tgcgtgaaga tacatcaggt
     1081 gcagctcgtt ggtggcttta atcagctcct gctcggcgct ctcggtaatg gtgtagtaat
     1141 gatacggatc ctgattgatc acctgaccgt tggcctgcac ataggcgttt tgcagcggat
     1201 ctttttcatc cagccattta ccgtcaaact ggcctttgtt ttccagacgc gctccgctga
     1261 ttttcagcag ctcgcctgca atttccggct gcggtaagct gtattcagta tcttccgtct
     1321 ggatcatcca gcccagaatg gtggtgtcat caaaagtgtc tttaagggta tagcagccgt
     1381 tttcgaccac catctccagt tcgcgcgtcc actgttgccc ttgcggcaac ggggaatgaa
     1441 tcacgttctg ttccgcaata cggactttgt tgccatgtaa ttgggtaatg atggcgacat
     1501 ggccagtgtc tttaaattca ccgcctttat cccagataag aagcgcaccc gcgaccggcg
     1561 cacgcggcga gccgttagga aatgcctgca atggcaggat gttgtcatta accacttcac
     1621 gcaggaagcg cagcgagaaa atctcccacg ccatacccac gtcagtaaag accacaccgt
     1681 agttcagaaa gagaaaacgg cgagcaaatt caacgcattg ccatttgtgg cccatatatt
     1741 cgtcgtcgat atagctacgg aatacggcgt catcttcgta ttcctgcgga tcgagagaac
     1801 tgtaatctga agagtagatt gctaccccac ctggggcgta gcccaataat gtcccgaacg
     1861 gggcatcctg gctggtcgtt cctttgctca tcactttacc tttaacaata caacctaagc
     1921 agttggcgta attgttgtgc tctgattacc tgctcagcga cattaaccgg acagaggtca
     1981 gactaatcat acacctcatc gcagcggctg gcgcaaaggg ttaaaaaatt cacattctgt
     2041 acagcaagtg acctgctaca ctgcttcaac actaccactc agaaggcaac tcactatgac
     2101 agacaatact tatcagcccg cgaaagtctg gacgtgggat aaatccgctg gcggcgcgtt
     2161 cgccaatatc aatcgcccgg tttctggtcc gacgcatgaa aaaacgctgc cggtgggcaa
     2221 acatccatta caactttatt cgctgggaac gccgaacggt cagaaagtaa cgattatgct
     2281 tgaggagctg ctcgcgctgg gcgttactgg cgcagagtac gatgcctggc tgatacgcat
     2341 aggcgatggt gatcaattct ccagcggctt tgtcgaagtg aacccaaatt cgaagatccc
     2401 ggcgctgcgc gatcataccc ataatccgcc gatccgcgtg tttgagtcag gatcgattct
     2461 gctttatctg gcggagaaat ttggctactt cctgccgcag gatctggcaa aacggactga
     2521 aacgatgaac tggctgttct ggttacaggg tgctgcgccg ttcctcggcg gtggttttgg
     2581 tcacttttac cattacgcgc cggtgaaaat tgagtacgcc atcaatcgct ttaccatgga
     2641 agccaagcgc cttctcgacg tgctggataa gcaactggcg cagcataagt ttgttgcggg
     2701 cgatgagtac accattgcgg atatggcgat ttggccgtgg tttggcaatg tggtgttagg
     2761 cggcgtatat gatgccgcag agtttcttga tgcgggcagt tataagcatg tgcaacgctg
     2821 ggcaaaagaa gtgggcgaac gtccggcggt gaaacgtggg cgtattgtta accgcaccaa
     2881 cgggccgctg aacgagcagt tgcatgagcg ccatgacgcc agtgattttg agacgaatac
     2941 ggaagataag cgtcaggggt aagggttagc gttcgtcaca gcaagccatc caggccggat
     3001 aaggcattca cgccgcatcc ggcaatcgag cacaatgcct gatgcgacgc tgccgcgtct
     3061 tatcagacct acgaaagcta atcatcacgc gctggtaatg tcgtaatcca tttggcgcag
     3121 tgcgtctaat gtggctttgg cttcatcttc gtcgatgatg ctcatggcaa atccgacgtg
     3181 taccagcacc cactggccca gtagatcggc agggttacct tcacaaatca gggcgatatt
     3241 cacgtcgcgc ttgataccac atacttcaac ctgcgcaagc tggtgaatat cttcaccgac
     3301 agccagcacc tggcctggaa cgccaataca cattgttaac tccggttatt caacttcaat
     3361 acttttgacg atcagcgaat cgccggtatc gacacgcaac cgctcgccat gacagagcgg
     3421 acactgcgca tcgtgctgat gaatctccac cacctggctg caatcccagc accaggcctg
     3481 ggcgggttta tagatgatat gtaaatcgca cccttgcgcc accgttccgt ggcagacaat
     3541 ttcaaaacta aaacggacgg cgctctcctc aacgcaggag agcgcgccaa tttccagcca
     3601 cacggcggtg acgcgcttaa catcgtgctg ctccgcctgc cgctggataa tttcaacggc
     3661 gctctggcaa agcgacaact catgcatttt cgccactcct gcgaccaaac agcagggcgc
     3721 gacgccctgc gtgtggtaca tccggattgg tgaccggcag cgaaaggatc attcgtgcgc
     3781 agtcatccgt caggcgttga ccctcttcaa tcgacatact gtgcgaaagc ggtgacatca
     3841 gcgaacagga gaggtattgc gaaacaccgt ccagttcgcc aacggtaaag gtcatggtgc
     3901 catacggcag ttgcagaccg attttttcac tcactttgcg cagcggccag agttgatccg
     3961 ggccggggaa gataactgca ctcagcatcc acggggtgat cacacacccc gtccactgac
     4021 cttcgaacag cgtaaaatca gaaacataca ccggcattaa aggatgcaga aaagagagat
     4081 cgtgcatcga acgccgggca atttcttcaa acgctgcctg tacttgcgcc ttcggggagg
     4141 tctggaaacc tgctatctct tcagtcatga atcgcctccc gtgggatggc ttccacgcca
     4201 gattcacgca gcgcagccag aacctgctca agcgcaggtt caatcatcgc ttcgaccgtc
     4261 ggcgtcaagc cgatgtgcgg ctccagtgat tccggaatta cgccgaccag ggtcagcttt
     4321 ttcggaaact cgccggtgaa gcgcagggcc gacaagacgt cggccaggcc aagctgatgc
     4381 ggagagattt tgttggtaaa caacgccgga acttcttcat cccgcaggat catcatcgtt
     4441 cccggcgcgc tcttcttcga cacaatggca tccgcaataa tcagatgatc gcgatttgcc
     4501 atgtcgccaa gcagctccat tcccgccgtg ccgccatcga ggatctcaac ataatccggc
     4561 agaatgtatc gttgctctaa cgcttcgaca atccgcacac cgatggcttc atcggtcagc
     4621 aaaatattgc cgacccctaa gactaaaata cgcattacag aaccttcact gaaaccactt
     4681 cgttgccgtc agcatccact acgtgtaccg cacaggccat gcacgggtcg aaggagtgaa
     4741 tggtacgcac cacttccagc ggtttattcg gatcggcaac cggtgtaccc accagcgact
     4801 gctcgtaagg accgacgtca tcattgaagt tacgcggacc agagttccag gttgatggaa
     4861 caaccgcctg gtagttgctg atgataccgt ctttaataac catccagtga gagagcatac
     4921 cgcgcggcgc ttcgaggaag ccaacacctt tgaactcacc cgttgccgga atgttcggct
     4981 tcacaaaggt ggtgtgatcg cctttgccga tattggtgat cagtgcactg tattggtttt
     5041 gcaggatatc ctgcaattca cagcagtgaa cggtacgacc aataatacgg cccagcgtgg
     5101 agtgcagctg tgccacttcc agcgtgttgc cagtcagttt ctgataaatc gcaacgattt
     5161 cattcagttt gttctgggta gattcgcgac ctgccgccag tttcaccagc atattagcca
     5221 gcggacccac ttctaccgtt ttgccgtaga aagtcggtga tttcacccag gaatatttcc
     5281 cgtcgtcaga ccaaccatca taagccggaa tggtggtacc ttcccacggt gcctgcggcg
     5341 cttcgtcttt ataccaggag tgcttcgcgc tttcctgaat gcctttgatc aggtattcat
     5401 cggaatgaga agtgatcgga cgatacgagg acagatccgc attctcaatg tagccgcccg
     5461 ggaacaggaa gctgccgttt ttactgtcgg tcgggaattc cggcacgctc aggtagttca
     5521 ccgcaccttt accgcgcgtc agccattccg ggtagaacgc agcaataact gcggtatcaa
     5581 ccttataaac ctgttcaaca aagtcgctca gcttgtcgat gaaagacttg atgtacatca
     5641 ggcgctcaag gttcagcacg cccagaccgt cgaggttgat tgggttagcg accccaccta
     5701 ccgccaggtt ctgaatgtgc ggcgttttac cgcccagcag cgccacgacg cggttagcgt
     5761 cacgctggca ctccagcgcc tgcaggtagt gcgctaccgc gatcaggttc acttccggcg
     5821 gcagtttcat cgccgggtga ccccagtagc cattggcgaa aatacccaac tgaccgctgg
     5881 caaccagatc tttgatcttg ttctgaactt tagtgaactc ttccggactg ttcagatgcc
     5941 aggtcgaaac gcctttcagc atttcggagg ctttggttgg gtcagcttgc agtgcagaag
     6001 tgatgtccac ccagtccagc gccgaaagct gatagaaatg aacgatatgg tcatgcgtgg
     6061 tgtgcgcagc cagaatgatg ttacggatgt attgcgcgtt aaccggaacg tcgatattca
     6121 gcgcactttc tgccgcacga acggaagaca gcgcgtgagt ggtagtacat acgccacaga
     6181 tacgttgcac aatcatccat gcatcgcgcg gatcgcggtt tttcacgatc tcttccatgc
     6241 cgcgccacat ggtaccggaa gcccatgctt tcgaaacgac gccattttcg atttcgcaat
     6301 cgatgcgtaa atgcccctca atacgggtta ccggatcaat agtaattctc tggctcatgc
     6361 tttgctcgcc tcatgacgat tatgatcgtt ttgttttaaa ggaggaagta tcggcagtag
     6421 acgaatgagt acgatgtaag cgcaaatctc aatagccaca aaaccaatag aaatcaacag
     6481 ttcttcccag gtcgggaagt aggcgtaacc gccgcccggg ttgaatgcca ccagcgaata
     6541 ggtcagacgc caggttgcac aacctaacag cgcgctcagt gccgacagga acagcatgcg
     6601 ggaatcatta cgcagcttcg ccacacgcag aaccaccagc gggaagagca tcagcaggac
     6661 ttcaatccag aacatcacgg agtagaagtc accggcaaac gctaacgaca gcttgtcgcg
     6721 atagataagc tcgccaaagc gcagcacgat gaaaatcgcc agcaacacac tgatggtgtt
     6781 ggtcagctta acgaacagac tcttttcatc cggaccgttg ccacgcagac ccgcctgcac
     6841 cagcgaacct tcaaagatga caatcgagaa gcccatgatg aacgccgtca gcagcgagaa
     6901 cagcggcaac atttcatagc tctgccacag cggatgcacc ttgtagcccg ccgagatcat
     6961 cagcgacccc attgaagact ggtgcatggt cggcagcagc gcaccgagcg cgatgatgaa
     7021 gaacatcacc ttgttcagac gctgtagcga caccttccag cccagacgtt caaacagtgc
     7081 cggagcaaac tccagagcca tcacgccgat gtagatggtc atacagaccg ccgtctcgaa
     7141 cagtaccgag ttcacgttga agtgacccgg aatgtagaag tacggcaggt tccagtagcg
     7201 acccacgtcg atagtgatcg acaagccacc cagtgagtaa ccaaacagac tcgccaacag
     7261 cgccggacgc accagcggat ggtattgccc acggttaaag acgtataccg cccacgccag
     7321 cgcccagccg ccacaggcaa agccggtgcc aatcagcagg tcaaacgcga tccacacgcc
     7381 ccacgggaag ccgccgttca ggtcagagac agagcccaga ccgaacacca gacgcttcac
     7441 aatcaggagc atacagatga cgattaacgg tccaaaaatc atgaccggtt tactgatgat
     7501 tttgccgccc agcggttttg gatcatgact catgatcgtc tcctccgtcg tgatggtcgt
     7561 ttttggtgtt gcgacgaacc agcacggtta agcccgccag cacagccagt ggtagcatca
     7621 tgcctttata cagggtgtgt tgaatatttt cggaacgcgc accggtagaa agatcgtcca
     7681 gtttcggcag gtcgagattt tcataaggca cacccgtcag taccagaacc tgagtaccgc
     7741 cgccctcttt ctcgccgtac agatgcggat aatatttcgg caccgtatgc aggtaagtgt
     7801 cgccagattt cagcgtctga cgcggatagt ggtattcgct gccaggcttc agcgccagac
     7861 gttttttcgc ctccgccatc agctcttcac gcgtaccaaa aatcaccgcg cccgccgggc
     7921 acacttctac gcagccaggc agaccgcctt tatcgagacg ttccacacct ttctggttgc
     7981 acagctcgca cttatgcagc gcaccaaacg ggttgttgta gtcgtacttc ggcacgttgt
     8041 acggacaggc gaccatgcag taacggcagc cggtgcacac gtctttgtcg taatgaacaa
     8101 tgccggtttt cggatctttt ttcagtgcag agaccgggca cacagagaca cagttcggat
     8161 cgacgcagtg catacactgt ttcttaatgt acgcgtagcc gttctcctcc tggtctttgt
     8221 tgacccctgt gccgctggtc cacacctgaa tgatgttatt ggtatacggc gacagtttgt
     8281 cgttgttcga ccaggtctgt tccccttgcg ggttacgttc agggaagttg atatcctgac
     8341 acttggtgac gcaagcctgg cagcctacgc acaaggtcga gtcgtacaac atccccagcg
     8401 atcccggaat tggcgggcgg ttttcagcag ccgcatgact gacagacggc agcgcgcccg
     8461 tcagcaatgc cccgcaggag gctgctttaa taaaattacg tctgttcacg gttattctcc
     8521 ccgtgagtca gcgttatctt tcttttgctg acgacccagt tcacgcaccg ccatcacact
     8581 gacaccggca accagcccaa tcacaccgcc gagcaaacca atagcgcctg cagagacgtt
     8641 gccgccctct ttagcgttaa catccggttt ctgtgaacgc ggagtctgat tttcgacgtt
     8701 ggcaagctga tggatgcctt tatggaagcc gataccttct tcgttacagc catagcacgg
     8761 gtgaccaatc gccaccggcc acacaccgcc aacatcgcag aattgcagcg ttgagcagtt
     8821 gccgtaagtt tctggccctt tacagccgag gtggtacagg caccagcctt cgcggtggcc
     8881 ttcatcaccg aactctttgg caaaacgacc agcatcgaag tgcgggcgac gttcgcagtg
     8941 ttcgtgaatc agacggccat aggcgaaggt cggacggttt ttgtcatcca gtttcggcgg
     9001 tttgccgtaa gtgatgatgt gcgcaacggt cgcgaggaag ttgtgcgggt tcggcgggca
     9061 gcccggaatg ttgataacgg ttttgcctgg cagaacttct tgcaggctga ctgcgccagt
     9121 tgggttaact ccagctgcgg caacaccgcc ccacgcagag caggaaccga tagcgataat
     9181 ggctgctgcg ccttccgccg ctttgcggat gtgatccaca atcggctcac cggcaaccat
     9241 gcaataaata ccgttatctt ttaatgggat ggaaccatcc accactaaca catactgccc
     9301 tttgtacttc tcgagagcgt tatgtttgtt ctcttcgacc tgatgaccga aggcggcgga
     9361 aagcacttcg tgatactcca gagagatagt ctccagtacg aggttttcta ccgttggatg
     9421 cgttgcacga agcagagatt ccgtacaacc ggtgcactcc tgcgcgccaa tccagataac
     9481 tggcggacgc tgcgggttag taaccgattc ggccatctct gcagcggctt tgctacttaa
     9541 ccccatggtg gcggctaatg ctgcacaaag cttcatgaaa tcacgacggt taatgccgtg
     9601 agaatggatg agggtgttat ctccagtcat ttatagttat tccgttgcga agacctagca
     9661 tatattttgc ctcaatcgca aaatcaataa tgcgatcgat gcgccattta ccacacattt
     9721 attatggtta tcgatatcat gatactgcgg cggggcgcta aaacgaaggg aagaagcatt
     9781 agtgtaatta attaaagcaa gataatacgt atgtttgatc aattttcgag gcgataaccg
     9841 gccataaaaa agcgcaacgt agaacaggaa ttattctcac gaaagaaatc cattaccatc
     9901 tctttatcca gtccatagcg gcgcgtcaga atacccgctt cccaggcgct aagctggcga
     9961 tctccggtct tttcgtacat ccggtgtgag tagcctaata caaaaccgcg tttataatcg
    10021 gcacagaatt ttgtcacatt aaccgcactg tcggcatgcg ttgcttttaa tcccgccatt
    10081 aagcctttac caaaatggtt attcatcgtt cacctcattc gctatatatc gtcatatata
    10141 gcgatatttt aggcaaagaa cgacttttgc gcacgctatt ttttcatgct tgtttaagac
    10201 gtaatcccgt aagagataaa cctatcagga gagatattta tcgaaccatt tcagtgtcct
    10261 ttgccaggca agatcggcgg cagatttgtc ataacggggc gtggaatcat tatggaatcc
    10321 gtgattaacc cccggataga tatacgcctc ataaacctta ttattggctt tcaacgccgc
    10381 ctcgtaagca ggccagccct cgttgattcg ggtgtccagt tccgcgaagt ggagtagtaa
    10441 aggcgcttca atcttcgcca catcggcagt gggtgcctga cgaccataaa acggcaccgc
    10501 gcaggccagt tccggatacg cgacagccgc cgcgttcgat acgccaccgc catagcaaaa
    10561 tccggtaata cccactttgc ctgtcgcttg cggatagcgt tgcataaact caattgcggc
    10621 aaagaaatca ttcatcagtt tggttggatc aacctgctgt tgcagctcac gacctttatc
    10681 atcatttccc ggataacctc caacggaact taagccgtca ggtgccaggg cgatataccc
    10741 cgccttcgcc actcgccgtg ccacatcttc gatatacgga ttcagtccac gattctcatg
    10801 caccaccacc acggctggcg ttttgccgct catttttgcg ggtttcacca gataaccccg
    10861 tacctcgccg tgaccatttg gcgaaggata cgtgatgtac tcagcaacaa tctccgggtc
    10921 ggtaaactct acctgagtcg ccagcgcata atttggcttg agcaaatcaa acaacgctaa
    10981 tgccgtcatc ccgcccaccg catacttcgc cgcaaggttg aggaactcac gtttcgagat
    11041 tttcccgtga gcgtaatagt cgtagtaatc caacaactct tgtgggaaat ctttggcggt
    11101 taaacgcggc atcgttgcac tcctcagctg gtgttttttt aagcaaagca taagcacgtg
    11161 tttttgccca gtttttcgtc actctgtgag ccagactacg ggatacgcgc tggcgaatcg
    11221 ctaaactaga aacattgttt cgaaattgaa cggtggaaag gagaggtcat ggtctggtta
    11281 gcgaatcccg aacgttacgg gcagatgcag taccgctatt gcggaaaaag cggtttacgc
    11341 ctgcccgcgt tatcgctcgg tttatggcac aatttcggtc acgttaacgc gctggaatca
    11401 cagcgtgcga tcctgcgtaa agcgtttgat ttgggcatta cgcactttga tttagccaac
    11461 aattacgggc cgcctccagg aagcgcagaa gagaactttg gtcgcctgct gcgggaggat
    11521 tttgccgctt atcgcgatga actgattatc tctaccaagg ctggctacga tatgtggccc
    11581 ggcccttacg gctctggcgg ttcacgtaaa tacctgctcg ccagcctcga ccaaagcctg
    11641 aagcgtatgg ggcttgagta tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg
    11701 ccgatggaag aaaccgcctc tgcgctggct catgcggtac aaagcggtaa ggcgctgtat
    11761 gtcgggatct cctcttactc gccagagcgg acgcaaaaaa tggtcgagtt gctgcacgag
    11821 tggaaaattc cgctgttaat tcatcaacct tcgtacaatt tactaaaccg ctgggtggat
    11881 aaaagcggcc tgctggatac cctgcaaaat aacggcgtgg gctgcattgc ctttactcct
    11941 ctggctcagg gattgctgac cggaaaatat ctcaacggca ttcctgaaga ttcacggatg
    12001 catcgtgaag ggaataaagt tcgtggtctg acgccgaaaa tgctcaccga agccaacctc
    12061 aacagcctgc gcttattgaa tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg
    12121 gcgttaagct ggttgctgaa agatgagcga gtgacgtcgg tattggttgg tgccagccgc
    12181 gcggagcaac ttgaggagaa cgtgcaggcg ctgaataatc tgacatttag caccgaggag
    12241 ctggcgcaga tcgatcagca tatcgccgat ggcgagctga atctgtggca ggcgtcttcc
    12301 gataaatgac ctgttaataa cgggcgcgag aaccgcgccc gaatcagatc agtgattatg
    12361 acgagtcagt cggtcaagat agcccattac aaatgcagag agcacaaacg tcagatggat
    12421 aatgacgtac cacatcagtt tgttatcagg gacatttttc gcatccataa agacgcgcag
    12481 taagtgaatg gaagaaattg ccacaatcga cgctgctact ttgtttttca gcgacgttgc
    12541 gtccattttc cccagccagt tcagcttctc tttgttctcg gagatatcga gttgtgagac
    12601 gaaattctca taaccggaaa acatcaccat caccagcaaa ccgccaacca gcgtcatatc
    12661 caccagcgac agcaacacga ggatcaaatc tgattccgcc atcgagaaga tattcggcag
    12721 tacgtgaata atctcctgga agaacttcag cgccagggca actaacgcaa gcgaaaggcc
    12781 aaagtacacg ggggcaagca gccagcgaga agcatacatt gcattttcaa gaaaacgttc
    12841 catacattcc tgtcgtgtag ttaaaacggg caacagtata tcgcaattga gtaaacagcg
    12901 cgcaacagca aatcaacata tccgttaaac ctttgtcgca taattctgag ggttagggat
    12961 cttcctggtt cccttatcaa cgcatcgaga gcgtctaccc ttaaaggacg ttgaacctga
    13021 agggagaaaa acgatgtctc atttaaaaga cccgaccacg cagtattaca ctggtgaata
    13081 tcccaaacag aaacaaccga cgccaggcat ccaggcgaag atgacaccgg taccggattg
    13141 cggcgagaaa acctatgttg gtagcggtcg cctgaaagat cgtaaagccc tggtgacagg
    13201 gggcgattcc ggaatcggtc gcgctgccgc catcgcttac gcgcgtgaag gggctgacgt
    13261 ggcgatcagt tatcttcccg tggaagaaga agacgctcag gatgtgaaaa agatcattga
    13321 agaatgcgga cgcaaagccg ttctgctgcc aggcgattta agcgatgaga aatttgcccg
    13381 ttcgctggtt cacgaagcgc ataaggcctt aggtgggctg gatattatgg cgctggtcgc
    13441 cgggaaacag gttgccattc ctgatattgc agacctcacc agcgaacagt ttcaaaagac
    13501 ctttgccatt aacgttttcg cactgttctg gctaacccag gaagcgatcc acctgctacc
    13561 gaaaggtgca agtatcatca ccacttcgtc aatccaggca taccagccaa gtccgcattt
    13621 actggactat gcggctacga aggcggcgat tctgaactac agccgtggcc tggcaaaaca
    13681 ggtctcggag aaaggtattc gggtgaatat tgtcgcgcca ggcccgatct ggacagcact
    13741 gcaaatttcc ggcggacaaa cgcaggataa gatcccgcag tttggtcaga aaacgccgat
    13801 gaagcgtgcg gggcaaccgg cggaactggc ccctgtatat gtttatctgg caagtcagga
    13861 gtcgagctac gtcaccgcag aagtgcacgg cgtgtgcggc ggcgagcatt taggttaaaa
    13921 aaatgcccgg ttgtgaaaag caaccgggca tcattgtgaa ttcactctta cctgatgtgg
    13981 cgaaaacgcc ttatccgcct acaaacgcat gaagattcaa catgttgcaa tagattttgt
    14041 aggcctgata agcgtagcgc atcaggcatt gagcacctaa tgccggatgc ggcgtgaacg
    14101 ccttatccgg cctacaaacg cacgaagatt caacatgttg caatagattt tgtaggcctg
    14161 atgagcgtag cgcatcaggc aattctactt tacttcgctt tggcggtttc ttcaccgacc
    14221 agacctatct tcaggtaacc cgcctgatgc agcgtatcca ttaccttcat caacgtctcg
    14281 taatcgacgg ttttgtccgc tcggaagaag atggtggtgt ctttcttacc ttcggttaac
    14341 gcattcaacg ccgtaatcat tgtttcatcg gtgaccggat cgttaccgat aaacatcgag
    14401 ttgtctgcct tcaccgacag ataaaccggt ttttccggac gcggctgcgg cgtgctggta
    14461 gaagcaggca agttcacctt cacatctacc gtcgctaacg gtgccgccac cataaagata
    14521 atcagcagaa ccaacatcac gtcgataaac ggcgtcacgt tgatatcatg catttcgccg
    14581 ttatcgtcga ggttttcgtt aagatgcatt gccatcggat attatcctgc acgtaatttt
    14641 tgtgcgacac gaaccggatg cgcagcggcg ctggcttcca gatccaggtc acggctttgc
    14701 agcaacaata cctgcgctgc gacatcaccc agcatcgctt taaagccgcc aatctgacgc
    14761 gcgaatacgt tatagataac gaccgccgga atcgctgcca ccagaccgat tgccgttgct
    14821 aacagagctt ctgcgatacc cggcgcaacg actgccaggt tagtagtctg cgtttgcgcg
    14881 ataccaataa agctgttcat gatgccccag accgtaccaa acagcccaac gaacggcgaa
    14941 atcgcgccga tggttgccag atagccgtta ccgcggccca tttgacgacc tactgcggcg
    15001 acccgacgct ccagacgaaa gctggtacgt tctttaatac cttcgttatc gtcgctgcct
    15061 tctgacagtt ccagctcatt ctgcgcttca ttgagcaaat gcaggcttaa gcttttgcta
    15121 ccaaaatcag cggcgatatc gttggcctgg tttaaggaac gcgcttcagc cagcagttgc
    15181 tgctcgcgct taaggcgacg cttctgattg aagaactcta cgctcttact gaagaagatt
    15241 gcccaggtga ctacggaggc caaaataagc ccaatcatca cgcacttaac gacaatatcg
    15301 gcgtgctgat acatacccca gacggaaagg tccgtctgca ttaaattatt acccactgtg
    15361 tatctccagg acgcaagtca caaaatctgc gcataataat atcaaaacga cgtcgaattg
    15421 atagtcgttc tcattactat ttgcatactg ccgtaccttt gctttctttt ccttgcgttt
    15481 acgcagtaaa aaagtcacca gcacgccatt tgcgaaaatt ttctgcttta tgccaattct
    15541 tcaggatgcg cccgcgaata ttcatgctag tttagacatc cagacgtata aaaacaggaa
    15601 tcccgacatg gcggacaaaa agcttgatac tcaactggtg aatgcaggac gcagcaaaaa
    15661 atacactctc ggcgcggtaa atagcgtgat tcagcgcgct tcttcgctgg tatttgagag
    15721 tatggaagcc aaaaagcacg cgacgcgcaa tcgcgccaat ggcgagttgt tctatggacg
    15781 gcgcggaacg ttaacccatt tttccttaca acaagcgatg tgtgaactgg aaggtggcgc
    15841 aggctgcgcg ctatttccct gcggggcggc agcggttgct aattcgattc ttgcttttgt
    15901 cgaacagggc gatcatgtgt tgatgaccaa caccgcctat gaaccgagtc aggatttctg
    15961 tagcaaaatc ctcagcaaac tgggcgtaac gacatcgtgg tttgatccgc tgattggtgc
    16021 cgatatcgtt aagcatctgc agccaaacac taaaatcgtg tttctggaat cgccgggttc
    16081 catcactatg gaagtccacg acgttccggc gattgttgcc gccgtgcgca gtgtggtgcc
    16141 ggatgccatc attatgatcg acaacacctg ggcagccggt gtgctgttta aggcgctgga
    16201 ttttggcatc gatgtttcta ttcaagccgc caccaaatat ctggttgggc attcagatgc
    16261 gatgattggc actgccgtgt gcaatgcccg ttgctgggag cagctacggg aaaatgccta
    16321 tctgatgggc caaatggtcg atgctgatac cgcctatata accagccgtg gcctgcgcac
    16381 attaggtgtg cgtttgcgtc aacatcatga aagcagtctg aaagtggccg aatggctggc
    16441 agaacatccg caagttgcgc gagttaacca ccctgccctg ccaggcagta aaggacacga
    16501 attctggaaa cgagacttta caggcagcag cgggctattt tcctttgtgc ttaagaaaaa
    16561 actcaatgat gaagagctgg cgaactatct ggataacttc agtttattca gcatggccta
    16621 ctcgtggggc gggtatgaat cgttgatcct ggcaaatcaa ccagaacata tcgccgccat
    16681 tcgcccacaa ggcgagatcg attttagcgg gaccttgatt cgcctgcata ttggtctgga
    16741 agatgtcgac gatctgattg ccgatctgga cgccggtttt gcgcggattg tataacattg
    16801 ccacttttgg acaattttgc agacatttta ttgtgaaaag tcttaaattg ttgcgtccgg
    16861 gatcaaggcg tcccggacga ttcaggagta caataggcag ataaaggctt aaacgctgtt
    16921 ccacaggaaa gtccatggct gttattcaag atatcatcgc tgcgctctgg caacacgact
    16981 ttgccgcgct ggcggatcct catattgtta gcgttgttta ctttgtcatg tttgccacgc
    17041 tgtttttaga aaacggcctg ctgcccgcct catttttgcc aggcgacagc ttgttgatat
    17101 tggctggcgc attgattgcc cagggggtta tggattttct gcctacgatt gcgattctga
    17161 ccgccgcagc aagtctgggc tgctggctaa gttatattca ggggcgctgg ttagggaata
    17221 ccaaaacggt gaaaggctgg ctggcacagc ttcctgctaa atatcatcag cgcgccacct
    17281 gcatgtttga ccgccacggt ctgctggcgc tgctggctgg acgttttctt gcatttgtcc
    17341 gtacgctgct gccaaccatg gcgggaattt ccggtctgcc aaaccgccgt ttccagtttt
    17401 tcaactggtt aagcggattg ctgtgggtca gcgtggtaac cagtttcggc tatgccttaa
    17461 gtatgattcc gttcgttaaa cgccatgaag atcaggtaat gacgttcctg atgatcctgc
    17521 caattgcctt gttaaccgct ggcttgttag gcacgctgtt tgtggtgatt aaaaaaaaat
    17581 actgtaacgc ctgacgattt tccctgttcc cggttgctgt accgggaacg tatttaattc
    17641 ccctgcatcg cccgcattct tgccgcatct tcccccggcg tcacaccgaa gtaacgttta
    17701 aactcacggc taaattgcga tgcgctttca tagccgacgc gcatcgctgc tgcgctggcc
    17761 ttcatgccgt catggatgat catcatccgc gccttatgca gacggtaatt cttcaaatac
    17821 tgcaacggcg aggtgctggt gacagactta aaattatggt ggaacgccga tacgctcata
    17881 ttggcttctg ccgccagttg ctcgacgctc aggttttcgg tgtatttatt ctcaatccgt
    17941 ttcagcacgc ggctaatcag actgaagtga gtctggcgac tgaccagcgc cagtaacgcg
    18001 ccgccgcaag gtccggtcag cacgtagtac agaatttcgc ggatgatctg tttgccgaga
    18061 atacgcgcat ccagtggtcg ctccatcacg tcgagtaacc gctccgccgc gcataaaatc
    18121 tcttctgata acgtggcgga gttaatcccg ctggctgcca tcgacggctg gaaatgctca
    18181 tcttcgccaa tgtccatcaa cagttcctgt aactgcaaaa tatcgacatt gagacgcaac
    18241 cctgccagcg gcacctctga cgtggcatag gtttcgcact caaacggcaa cggcaccgtc
    18301 agcagcaggt attcattggc atcataacga aacacgcgtt cattgatata accgatttta
    18361 tgtccggaaa agagaattat gatgccaggc tcgtacatca ccggtgtacg tgcgaaaggc
    18421 gtctcgccat acaacaaacg cacatcgggc aacagttctg acaaactatt ttctttattt
    18481 ttcagtttat taactttatc cgccagcaag cggcaaatct cttcacgttt catatcgcgt
    18541 aatttcttag gaataatgcg gcaatttgat tgtgcgcaat tttgtagcat ttctccagca
    18601 ctctggagaa ataggcaaga cattggcaga aatgagcatt gagagccagg gcgctggcga
    18661 tcacaatgaa aaacatcagg cagatcgttc tctgccctca tattggccca gcaaagggag
    18721 caagtaatga acaactttaa tctgcacacc ccaacccgca ttctgtttgg taaaggcgca
    18781 atcgctggtt tacgcgaaca aattcctcac gatgctcgcg tattgattac ctacggtggc
    18841 ggcagcgtga aaaaaaccgg cgttctcgat caagttctgg atgccctgaa aggcatggac
    18901 gtgctggaat ttggcggtat tgagccaaac ccggcttatg aaacgctgat gaacgccgtg
    18961 aaactggttc gcgaacagaa agtgactttc ctgctggcgg ttggcggcgg ttctgtactg
    19021 gacggcacca aatttatcgc cgcagcggct aactatccgg aaaatatcga tccgtggcac
    19081 attctgcaaa cgggcggtaa agagattaaa agcgccatcc cgatgggctg tgtgctgacg
    19141 ctgccagcaa ccggttcaga atccaacgcc ggtgcggtga tctcccgtaa aaccacaggc
    19201 gataagcagg cgttccattc agcccatgtt cagccggtat ttgccgtgct cgatccggtt
    19261 tatacctaca ccctgccgcc gcgtcaggtg gctaacggcg tagtggacgc ctttgtacac
    19321 acggtggaac agtatgttac caaaccggtt gatgccaaaa ttcaggaccg tttcgcagaa
    19381 ggcattttgc tgacgctgat cgaagatggt ccgaaagccc tgaaagagcc agaaaactac
    19441 gatgtgcgcg ccaacgtcat gtgggcggcg acgcaggcgc tgaacggttt gattggcgct
    19501 ggcgtaccgc aggactgggc aacgcatatg ctgggccacg aactgactgc gatgcacggt
    19561 ctggatcacg cgcaaacact ggctatcgtc ctgcctgcac tgtggaatga aaaacgcgat
    19621 accaagcgcg ctaagctgct gcaatatgcc gaacgcgtct ggaacatcac tgaaggttcc
    19681 gacgatgagc gtattgacgc cgcgattgcc gcaacccgca atttctttga gcaattaggc
    19741 gtgccgaccc acctctccga ctacggtctg gacggcagct ccatcccggc tttgctgaaa
    19801 aaactggaag agcacggcat gacccaactg ggcgaaaatc atgacattac gctggatgtc
    19861 agccgccgta tatacgaagc cgcccgctaa gctttttacg cctcaaactt tcgttttcgg
    19921 gcatttcgtc cagacttaag ttcacaacac ctcaccggag cctgctccgg tgagttcata
    19981 taaaggagga acgtatggct aatccaaccg ttattaagct acaggatggc aatgtcatgc
    20041 cccagttggg actgggcgtc tggcaagcaa gtaatgagga agtaatcacc gccattcaaa
    20101 aagcgttaga agtgggttat cgctcgattg ataccgccgc ggcctataag aacgaagaag
    20161 gtgtcggcaa agccctgaaa aatgcctcag tcaacagaga agaactgttc atcaccacta
    20221 agctgtggaa cgacgaccac aagcgccccc gcgaagccct gctcgacagc ctgaaaaaac
    20281 tacagcttga ttatatcgac ctctacttaa tgcactggcc cgttcccgct atcgaccatt
    20341 atgtcgaagc atggaaaggc atgatcgaat tgcaaaaaga gggattaatc aaaagcatcg
    20401 gcgtgtgcaa cttccagatc catcacctgc aacgcctgat tgatgaaact ggcgtgacgc
    20461 ctgtgataaa ccagatcgaa cttcatccgc tgatgcaaca acgccagcta cacgcctgga
    20521 acgcgacaca caaaatccag accgaatcct ggagcccatt agcgcaagga gggaaaggcg
    20581 ttttcgatca gaaagtcatt cgcgatctgg cagataaata cggcaaaacc ccggcgcaga
    20641 ttgttatccg ctggcatctg gatagcggcc tggtggtgat cccgaaatcg gtcacacctt
    20701 cacgtattgc cgaaaacttt gatgtctggg atttccgtct cgacaaagac gaactcggcg
    20761 aaattgcaaa actcgatcag ggcaagcgtc tcggtcccga tcctgaccag ttcggcggct
    20821 aacgtgcaaa ttctcccggt ggcggtaatg ttccgctacc ggacttttca gaaatcattt
    20881 attcccctcg cgtcccgccc gttgttactc ttccttgttc aggaatgcca aatataagga
    20941 catcatcatg cagagccgga agctcttaaa agaacaactc atctatatcc gggataaacg
    21001 caacggagag gtgaaaaaca gatgaaaata atacttctgt ttttagcagc cctggcaagt
    21061 tttaccgtac acgcacagcc cccctcacag accgtagaac aaacagtccg gcatatttat
    21121 cagaactata aatcagatgc caccgcccct tattttggtg aaaccggaga gcgggcgata
    21181 acttctgtgc gtattcaaca ggcgcttacc ctgaacgaca atcttacgct gccgggtaat
    21241 attggctggc tggattatga tccggtttgt gattgtcagg attttggcga tctggtgcta
    21301 gaaagcgtag cgataaccga aactgacgcc gatcatgccg atgccgttgt gcgctttcgt
    21361 atctttaaag atgataaaga aaagaccacg cagacactga aaatggtggc ggaaaatggt
    21421 cgttgggtca ttgacgatat tgtcagcaat catggcagcg tcttacaagc agttaatagc
    21481 gagaatgaaa aaacgctggc cgctttagct tcgttgcaaa aagaacagcc ggaagccttt
    21541 gttgccgaac tctttgaaca tattgccgat tatagctggc cgtggacgtg ggtggtttcc
    21601 gactcttacc gccaggcggt caatgccttc tataaaacca ccttcaagac ggccaacaat
    21661 cccgatgaag atatgcaaat agaacggcaa tttatttacg acaatccgat ctgttttggc
    21721 gaagagtcgc tattttcacg cattgatgaa attcgagtcc tggagaaaac cgccgattcc
    21781 gcccgcattc atattcgttt tacgctgacc aatggcaaca acgaagagca agaactggtt
    21841 ttacaacggc gcgacggtaa gtgggaaatc gctgatttta tccgcccgaa cagcggcagc
    21901 ctacttaagc agattgaggc aaaaaccgcc gccagattaa agcaatgagc tgaattaaat
    21961 aacaattagc cggaacaata aaaaagggaa cactatatga aaacgatttt caccgtggga
    22021 gctgttgttc tggcaacctg cgtgctcagt ggctgcgtca atgagcaaaa ggtcaatcag
    22081 ctggcgagca atgtgcaaac attaaatgcc aaaatcgccc ggcttgagca ggatatgaaa
    22141 gcactacgcc cacaaatcta tgctgccaaa tccgaagcta acagagccaa tacgcgtctt
    22201 gatgcacagg actattttga ttgcctgcgc tgcttgcgta tgtacgcaga atgataaaaa
    22261 aatccccggc agcatgtcag ttgccgggga ttttttttaa cgtccaaccg ccgctttagg
    22321 gcgtttcttc gcaccagcat tcaccggacg agattgcgta gacgacgctt tttttgccgt
    22381 agcaggcgtc tgacgctggg tcgccatcgg cgtatgtttc gtcaacgctg gacgagtatt
    22441 gcggttctga cgacgagcct cacgcatctc ttcaatggtt ggcgcaggca ctaagcaatc
    22501 gcgacggctg ccaatcagat gctttttacc cattgcttcc agcgcctgac ggattaacgg
    22561 ccagtttgcc ggatcgtggt aacgcaacaa cgctttatgc aaacgacgct gtttgtcacc
    22621 cttcggtaca aagacgtctt cactcttata accaatcttc gccagcgggt ttttcccggt
    22681 gtaatacatg gtggtcgagt tagccagcgg cgatgggtag aaattctgta cctggtcgag
    22741 acggaaacga tgctttttca gccacagcgc caaattcacc atatcttcat cacgcgtacc
    22801 ggggtgcgca gagatgaaat acgggatcag atactgctct ttacctgcct gtttcgagta
    22861 agtatcgaac agctctttaa agcggtcata gctgcccatg cccggcttca tcatcttcga
    22921 caacggccct tcttcggtat gttccggagc aatcttcaga taaccgccga cgtgatgggt
    22981 cgccagttct ttgatatagc gcggatcttc cacggcgatg tcataacgta cgcctgaggc
    23041 aatcagaatc tttttaatgc ctttcagatc gcgagcgcgg cgatagaggt tgatcgtcgg
    23101 ttcgtggtta gtgtccatat gcggacaaat atccgggtaa acgcaggaca aacgacggca
    23161 ggtttgttca gcgcgtggcg atttgcagcg caacatatac atgttggcag ttggcccacc
    23221 gagatcggaa attacgcccg taaaacctgg aacggtgtcg cggatcgctt cgatctcatt
    23281 aatgatcgaa tcttcggaac ggctctgaat aatgcgccct tcgtgctcgg tgatagaaca
    23341 gaaagagcag ccgccaaagc agccgcgcat aatgttgacc gaaaaacgga tcatttcgta
    23401 agccggaata cgggcattgc cataggccgg atgtggcacg cgcttgtatg gcagcgcaaa
    23461 aacgctgtcc atctcttcgg tagaaagcgg aatagcaggc gggttgatcc acacatagcg
    23521 gccgccgtgt ttttgcatca atgcgcgggc acagcctggg ttggtttcgt ggtgcagaat
    23581 acgcgaagca tgggcgtaca gcactttatc gcccttcact ttctcgaaag aaggcagcaa
    23641 cacgtaggtt ttttcccacg gtttcgggcg cggtggctgc acggttacgg ctttggcttc
    23701 ctgctttttc ggtgccaccg gtttgttatc cgcgcacggc aaatcttcgc catacggatg
    23761 cgggatcggg tcgattttcc caggggtatc aagacgagta gaatccacac cgctccagcc
    23821 tggcaacgcc tcttttacga taatcgcagt attacgcaca tcgcggattt cgctaattgg
    23881 ctcgcccatc gccagacggt gcgccacctc caccagcgga cgctcaccgt taccaaacat
    23941 cagcatgtcg gctttcgaat ccaccagcac ggaacggcgc acggtatcgg accagtaatc
    24001 ataatgcgcg gtacggcgca gactagcctc aataccaccg aggatcaccg gtacatcttt
    24061 ccacgcctct ttacaacgct gggtataaac cagtgtggcg cgatccgggc gcttacccgc
    24121 gacgttatct ggcgtgtagg catcgtcatg acgtaaacgg cgatcggcgg tataacggtt
    24181 gatcatcgaa tccatgttgc cagcagtaac accgaaaaac agattcggtt tacccagacg
    24241 catgaaatcg tctttgctgc tccagtctgg ctgagcgatg atcccaacgc gaaagccctg
    24301 cgcttccagc atacgaccgc aaatcgccat cccgaagctt gggtgatcga catacgcgtc
    24361 gccagtaacc aaaatgatgt cgcagctatc ccagccaagt tgatccatct cttcacgaga
    24421 catcggcaaa aacggtgccg gtccaaaaca ggcggcccag tactgcggcc atgagaacag
    24481 gtcgcgatcc ggttggatca gggagataga gctcattttg cttccagaaa tgataaaaaa
    24541 ataatcaaag gccggggatt ataagccgga acgaaagaga aatcgaaaag tattccatac
    24601 tcgccctcct cgggcgagta tgaagattac ggtaccggat tgactaacag ttgcccaatc
    24661 gagccacggt ccgccatttc cagcgtctga ctgttgaagt agaacgggaa gtgcgcccag
    24721 gaaggctgac cgaaataaac aagcagctcc acttgtccgt caacccaaac ggtatctttc
    24781 cagcctctgt cttccgggaa cggcatcgca ccattcacgt tacggatctg gaacatcacg
    24841 ccttcaatat ggaacgcttg cggctcgtcc gcgcgtaccg tccagcgttc ccacgttcct
    24901 tgctgcgcgg tgacatcaat acggttgacg tcccacagtt gtccattaat acccgggtca
    24961 tcacccagac tgatatcgcg actgcgaatc ggcgaaccgg ccatgatttc agttggcagc
    25021 aagcgcatcg gaagactgtc tgtgaccagc ggcagaaggc cggttgggcg cagcgttagc
    25081 accagggtag aaaccagaat gctggatggc tcaaagaagc cacgaatacg atcaacaatg
    25141 ctcgccgctt cgccacaggt gatcgacact tcatcaccgt tgctcatatc caccagaatc
    25201 tcgcggcgct cgcccggtgc cagcgaaagt tgcttcaccg atacaggagc aggcaggaat
    25261 ccctgatcgc cagaaatcac atgtaacggg cgaccatcgc tcatctgtag ttgatagcga
    25321 cgagagttcg acgcgttcag cagtcgcaag cgcacccagc cacgcgagac ttcaacgtac
    25381 gggctttgta caccgttaac cagcagcgta tcaccaacaa agccgccgct tcccggttcg
    25441 ttgtattctg gcgtaccaaa gttatccagc cgtttatcct gaataatgac cggaaaatca
    25501 tccacaccat aatggttggg gataggcagc gacttgctga cttcatcttc caccagccac
    25561 attccggcaa ggccgttata gacctgctga gccgtgcggt tgggagtatt ggcgtgatac
    25621 cacagagtag ctgcgttctg gcgaatgggc agtacgggtg cccagtcagc gtttggcgac
    25681 atcatccgtg ccggaccgcc catcagcggg cctggtacct gtagcccggc caccgtcatt
    25741 gagacatttt ctgtcaggcg gttgctgtaa ataagcttaa catcgtcgcc cttccagacg
    25801 cggatagtcg gccccaggta acgaccattg attccccaga ccgacgcgcg tgtccctggc
    25861 gtaaatgacc agtgcgcacg ttgtacagtc ataaacagcg gttgcccacg gcgagattca
    25921 agcagcggcg gaacgggtag cggttgctgt tgcccggctg cgctggcctt caggggaaca
    25981 gcgcctgcac aaagtgcaat ccccgatgcc tgaatgaact gacgccgact gagtgacata
    26041 tttgctccat gtaaaactgg ctaaagtaat accgtgcagg aaagtgttcc ccgcaaaaat
    26101 acagattgcc gacttaaacc tttccggcgg cttcgcgttc tgcgacttct ttatcgagct
    26161 cggcgatttt ttgttccatt atcgaacgac aatgggcagc cagctcacga acctgatctt
    26221 tgccatactg actgacgtca attggcggca gcatttcgac aatcaccaga ccgttgtgca
    26281 gtcgattaag attaatctta ttcgaagttg tagagacgca cacgggaata atcgggacgc
    26341 ctgctgcaat tgccgcgtga aatgctccag tcttgaacgg tagcaggccg cgaccacgac
    26401 tgcgggttcc ttccgggaac atccaaatgg aaatacggcg ttttttgaaa tgattcacta
    26461 cttccgcaat agtgccgtga gctttagtgc gattgtttct gtcgatcaat aagttgccgg
    26521 ttaaccagta caactgcccg aagaaaggga tccacagcaa gctcttttta cctaccgtca
    26581 ccgtcggcgg ttgcacaatg ttcgatgctg tcaccatgtc atagttgttc tggtggttag
    26641 cgatatagat agcattgccg tagctttcag catctgcagg tttgcggcac tcaactttca
    26701 ggccgaacag cggcgcaaga cgaccaaaca tatgcccaaa ggtggccaca tgtttcgggt
    26761 tacgcgggct gaaaaggcag taaatggagc cgaatacaca gactaagatg ctgtaaatca
    26821 cggtaataat aagacgaaag atatatagca taacaccctc tgaaggtctg agagactggc
    26881 attgtacgtc acctgaaatc aggttagggc attgttaaaa gccgctctct ggaaaagcgt
    26941 attgttaatc gcaacgcatg aataaacaac ggtttcaagg gaatttttat cgtttccggg
    27001 ggttgaatac gctgccggat gacgacttaa cgtttcatcc ggcgttcctt gcaagcggga
    27061 ggaaacagcg ccctccccgg catattactc ttcgctatca ccgctgctgg cacggcgagg
    27121 agagtcgatc tcaacacgat cgatacgctg caaaccgcgc atcaacgtac cgcggcgtcc
    27181 acgttcgcca gtgactttct gtaactcttc cgggcgcagt ttaattttgc gtttcccaac
    27241 atgaatggtc agcgtgcttt gcggcggcag aacgtacagt tgcgccagac catcttctcc
    27301 acgcgcggct tctgccgatg gaatgttgat aatcttgttg cctttgccct tcgacagctg
    27361 cggcagatcg cttaccggga acatcaacat acggcctgcc tgagtgattg ccagcagcat
    27421 atcggaagca tcttcaatta ccaccggcgg cataacatgg gcattttccg gtaaggtgat
    27481 caaagcctta cctgcacggt tacgcgccac cagatcgtta aaggtgcaga cgaaaccgta
    27541 acccgcatcg gaagccatca gcagtttctg atcgtcgctt tccatcagca tatggtcaac
    27601 ggtcgcccca ggcggcaacg ttaatttgcc ggtgagcggt tcgccctgac cacgcgccga
    27661 cggcagcgta atcgggtcaa tggcatagct acgaccggtg gaatcaacaa acactaccgg
    27721 ttggttgctc ttacctttca ccgccgcttt gaagctatca cccgctttat aattcaggcc
    27781 cggcgcgtcg atatcatggc ctttagcgct gcgtacccag cccatctgcg acagcacaat
    27841 ggtgacaggt tcagacggca gcatgtcgtg ctcgctcatc gctttcgctt cttcgcgttc
    27901 ctgcaacggc gaacgacgat cgtcaccgta ggcttgcgcg tctgcctgca gttctttctt
    27961 cagcaggtta ttcattttac gctcggaagc caaaatgccc tgcaactggt cgcgctcttt
    28021 ttccagttca ctctgctcac cgcgaatctt catctcttcc agtttggcaa gatgacgcag
    28081 tttcagttcg aggatcgctt ccgcctgggt ttccgtaagg ccaaaccgca acatcagcgc
    28141 cggtttcggt tcatcttcat tacgaatgat ctcaatcact tcgtcgatat tgagaaacgc
    28201 caccagcaaa ccttcgagga tatgcaggcg cttgaggact ttctccagac gatagttcag
    28261 tcggcggcgc acggtatcgc ggcggaacac cagccattcg gagagaattt ccagcaggtt
    28321 tttcaccgcc ggacgaccat ccagaccgat catattgagg ttaatgcgat agctcttttc
    28381 cagatcggtg gtagcgaaga ggtggttcat cacctgatcc atatccacgc ggttggaacg
    28441 cggcacaatc accagacggg tcgggttctc gtggtcagat tcatcgcgca gatcgtcaac
    28501 catcggcagc tttttgttgc gcatttgcgc agcaatttgc tccagtacgc gcgcacctga
    28561 aacctgatgc ggcaatgcgc tgataaccac cgcgccatct tctttcttcc acaccgcacg
    28621 catacgcact gaaccacgtc cgttctcgta gattttacgg atctcggcgc gcgaagtgat
    28681 aatttccgct tcagtcggat aatccggccc ctgcacgata tccagcagct gatcgagcgt
    28741 ggttttcggc tggtcgatta atgcgattgc cgcctgagcc acttcacgca ggttatgcgg
    28801 tggaatatcg gtcgccatgc cgacggcaat accggtggtg ccgttaagca aaatgtttgg
    28861 cagacgggca ggtagcattt tcggctcctg caaagtgccg tcgaagtttg gcacccagtc
    28921 agccgtcccc tgccccaact cgctcaatag cagctcggaa tatttcgaca accgggattc
    28981 ggtgtaacgc attgccgcga acgatttcgg atcgtccggc gcgccccagt tcccctgacc
    29041 atcaaccagc ggataacggt aagagaacgg ctgcgccatc aggaccatcg cttcataaca
    29101 ggcgctatcg ccgtgcggat ggtatttacc cagtacgtca ccgacggtac gggccgattt
    29161 tttaaatttg gcgctggcat tcaggcccag ttcagacatc gcatacacaa tgcggcgctg
    29221 aacaggtttc agaccatcac caataaacgg caacgcacgg tccatgatca cgtacatgga
    29281 gtagtttaag taggcgtttt ccgtaaattc atgtagcgca aggcgctctg ccatatcgct
    29341 cattaattct gattcctcaa cttattcgcc agccttcaaa ggggcaatat tgccggagat
    29401 actacattat ctgccgcgtc gagtcacagg agatcagcgt gaatagcgcc gccggacgtg
    29461 ctccgacggc gataaaggca tcaatgtgcc ttgatataca actctttgct gtagacataa
    29521 tcctggggat tatgcagcgg aaaaccgcca acgtagggtt taagcagttt gattaacggc
    29581 tgatagtaga taggaatcag cggtgcctgt tggttgatga tcacttctgc ctgctgatac
    29641 aacgcattac gctttgtcgc atcagtgatc tgcgtggcct ggtttagtaa ggcgtcatac
    29701 tgcgcatttt tccagtgacc gacgttttct tcactatcgc ttttgagcgt gttcaggaag
    29761 ctggaagcat cattgtacgt cgcatcccac gactgccgag acagcatgaa atcaccggct
    29821 cgtcgggcat caagataggt tttccactcc attgtgcgca gcgtcacctg tgcacccagc
    29881 cattttttcc attcggaaga caacgctatc gcggtctttt catgcagatc gtacttgttg
    29941 tagaacagct caaagcgtag cggatgagag gcgtcgtatc ccgcctgttt cagcaaggct
    30001 tttgccatcg cgacgcgctc actcattggc ttttgcagtt catcgaacgt cgtcgcgcta
    30061 aagcctttta cctctggcgg cgtcagcgtg gttgcgggcg ttctcaaccc cagtaccttt
    30121 tgcgcaataa gctgtcgatc aaccgtaaga tatagcgccc gacgcacccg cacatcgtta
    30181 aatggcggtt tctcaaggtt gaagttgtaa tattcgctgt tcagacgcgg aataattcgt
    30241 agctcgccag gcagtgattt ttcaatggcg ggaatttgct gcgccggaac ccaggtgaga
    30301 tcgacctctc ccgcgcgata gcggttatag ccggtgaccg aattatccag cgccagatac
    30361 tcaacctgtt gcaatattgt atgttgcgca tcgcggtact ttggattttt gcgtgcagta
    30421 atcttttcgt taactaccca ctgatcaagc acaaaggcac cgttgtaaac catgttctct
    30481 ggcttactcc agctatcgcc atgtttagcg atgacatgat gaggaaccgg gaacagcgtc
    30541 ggccaggcga gcatcgtcgt gaaccaagga accggctgct caagcgtaac ttcaagagta
    30601 cgatcatccg tcgctttgac acccagcgat gtaacatctg ctttacccgc aacaatagcc
    30661 gcggcattgt taatgtgcgc ctgtgccaga tagccagcaa aagggcttgc cgttttcggg
    30721 tcaaccgcgc gctgccagcc gaggacaaaa tcctctgccg tcagaggctg accgtcggac
    30781 cactgcaaac cgctacgcag atggaaaata tagcgcctgc cgccgtccag tatctcccag
    30841 cgttcagcct gagcgggctg cacctggcct tcaccatcca tccataccag accttcaaac
    30901 agatccagca caatctgcgc ggcagtattc tcctccacct tttgcgggtc gagcgtacct
    30961 gggtcgctat gattgttgta acgaaagact tgttgcggag cgagcggtgt gttggtggga
    31021 acgtcagcag cgtagagagg agccgcactt accaggctga ccagccataa taaatttcgc
    31081 gtatacatgg tattccttac cggagaaatt ataagcccaa caacaatacg cctgtggcat
    31141 tgttcgctca aacttatcac gagtgatttg gctcacactc cggtaaagat tccccatcac
    31201 gctaacggca cataaacatc cgtttccagt cgctgctctg tcaccccttc ggcaagattg
    31261 gtgtagtgaa acagaatcgg tgctttacgc attttctcgc cgcttgcagg cagccagtgg
    31321 cgaataatgc cccatatcgt gtgtgaaata tcgtccagct cgccaacgtg gcgggccacg
    31381 gcataacgtc caccggtaag ttcaccattg ctcacaccat aacgattatc gggaatcggt
    31441 tcgctaacgc tgccgcagat atcaaagcga aacgcttccg gtgcggtggt ggcagggtcg
    31501 tcccaggcga cgccaaaagt ctggctttgg ttaacaggag aaagccccgt ctctttacgc
    31561 cacatgataa atttcgccac actgtagttg agcaactcag ggctacagcg gtgcggcaac
    31621 atcgccacag ggattgatgg gaaatcgata atgtttacat ccagtgtcag gtttgtcatt
    31681 ttgcctccct gcgaaattat tcccgggaac gcgtcccggg agcggtaaca attacggatt
    31741 tactttgcgg atctgtttga cgtcaatttc aacagaattc cagtctttat cgacttcacc
    31801 ctgaatctca accgtatctt tcggcgtcac cgtcacgccg ttccagcgtt tgtgatcgat
    31861 atcaacattg atagtaccgc tggcatcttt gaacacgtag agatcgtcag agatgcgttc
    31921 aacgatattg ccgcgcaggg ttacccaggt gtcgtcacgc agggattttg cgctttctac
    31981 agtcgttacg ctgccgttcg gcccctggaa tcctccggcc tgactttgcg ttgccgatgg
    32041 gccagaaaaa ccgccctgct ctgctgccat caccggtgcg ctgcacaggg ccattactgc
    32101 gattactgct gcgaattttt tcatgtttat tactcccttt aatgtctgtt tccgagcatt
    32161 taacaagata gtccttaaca acttcttaag ggaaaaaaat aaaatttagt gctgtacaga
    32221 gcgcgttaca acacggttta ctggcagcaa atacggttat cgcagggatg aaaaaatgcg
    32281 agttttactg atagaagatg acatgctgat tggcgacggc atcaaaacgg gccttagtaa
    32341 aatgggtttt agcgtcgact ggtttacaca aggtcgtcag ggaaaagagg cgctttatag
    32401 cgcaccttat gatgcggtga tcctggattt aaccttacca ggcatggatg gtcgcgatat
    32461 tttgcgcgaa tggcgagaaa aaggtcagcg tgagccggta ctgatcctga ccgcgcgcga
    32521 tgcgctgggg gaacgtgtag aagggctgcg tctgggagct gacgattatc tgtgtaaacc
    32581 ttttgcgttg atagaagtcg ccgccaggct ggaagctctg atgcgccgaa ccaacggcca
    32641 ggccagcaac gagctgcgcc acggtaacgt catgctcgac cccggcaaac gtatcgccac
    32701 gctggctggc gaacccttaa cactgaaacc aaaagaattt gccctgctgg aattactgat
    32761 gcgtaacgct ggtcgggtac tgccgcgcaa actgattgaa gagaaactgt atacctggga
    32821 cgaagaggtc accagtaatg ccgttgaagt gcatgtgcat catctgcgac gcaaactcgg
    32881 tagtgatttt attcgtaccg tgcatggtat tggctacaca ttaggtgaga aatgaaattt
    32941 acccaacgtc ttagtctgcg cgtcaggctg acgctaatct ttttaattct ggcctcggtg
    33001 acctggctgc tttccagctt tgtcgcctgg aaacaaacaa cggataacgt cgatgaattg
    33061 ttcgacaccc aactgatgct ttttgccaag cggttaagta cgctcgatct caacgaaatc
    33121 aacgcggcgg atcgcatggc acagacgcca aataaattaa aacacggtca tgttgatgac
    33181 gatgcgctga cctttgccat ctttacccac gacggcagaa tggtccttaa cgatggcgat
    33241 aacggagaag atattcccta tagctatcaa cgggaaggtt ttgctgacgg gcaactggtc
    33301 ggtgaagacg atccctggcg ttttgtctgg atgacctcac ctgatggcaa atatcgcatc
    33361 gttgttggtc aggaatggga ataccgtgaa gacatggcgc tggcgattgt tgccgggcaa
    33421 ttgatcccgt ggctggtcgc actgccgatt atgttaatca tcatgatggt actactgggt
    33481 cgtgaactcg cgccgctgaa caagctggcg ctggcgctac gtatgcgtga ccctgactcg
    33541 gaaaaaccac taaacgcgac tggcgtaccc agcgaagtgc gaccactggt tgagtcgcta
    33601 aatcaactgt tcgtccgcac acatgcgatg atggttcgtg aacgacgctt tacctccgac
    33661 gcagctcacg aacttcgtag cccgttaacg gcgctgaaag tgcaaaccga agttgcgcag
    33721 ctctctgacg atgatccgca ggcgcgaaaa aaagcactgc ttcaattaca ttacgggatc
    33781 gatcgcgcta ctcgtctggt tgatcaactg ctcacgctat cgcggctgga ctcactggat
    33841 aaccttcagg acgtcgcgga gatcccgctt gaagatctcc tgcaatcgtc ggtgatggat
    33901 atttaccaca cggcgcagca ggcgaaaatt gacgtgcgac tgacactcaa tgtccagggc
    33961 atcaaacgca ccggacaacc gctattgcta agtttgttgg tgcgaaattt gctggataac
    34021 gccgtgcgtt acagtccaca gggcagcgtg gtggacgtca cgctgaatgc cgataatttc
    34081 atcgtgaggg ataacggccc cggtgtgaca ccagaggcac tggcgcgaat tggcgaacgc
    34141 ttctatcgcc cacccggaca aaccgctacc ggcagtggac ttgggctatc gattgtccag
    34201 cgaatcgcca agctgcatgg catgaatgtt gagtttggga atgcggaaca aggtggattt
    34261 gaggcgaagg taagctggta acttctctgt ctatgagagc cgttaaaacg gctctcatag
    34321 attttactaa tagcaaaata taaaccgtcc ccaaaaaagc caccaaccac aaaccagaca
    34381 aataacgaac gagcatacat ataaccaaaa ctcaaacatt tcccgcacaa agagttttct
    34441 taagataata ataataagtg gcgtcagaag aaaaaatgct gcaatcattg cttgcaatat
    34501 acgatcttcg cctgagacgg aaggtatact gcaaatattg tctcccgagt caagcatcca
    34561 gtcatactta ttgaaagcaa aaaaagcatc tattaaccaa taagtaagca ttatatataa
    34621 cagagcttca aatattgttt gaatttcatg ctttaacata tgttagcact ctctaaaaaa
    34681 gtccagtcgg agatctggtt ttcgataccc tccagtaaat ctatttaaaa taatctgcgt
    34741 aacacgctga tattttcatt tttagttatt caacaccaca attatcaact cgcagaaagt
    34801 ccacaccaac gttgtaaatt aaccctgtgc tagttggaat gatgagaaat tgctatcagt
    34861 attgtcattt tcagaaaata tttatcctgc atcggtgagt cagagcaaga tcagactttt
    34921 gctaaattcg caaaagactt tgcacatttt gctaatttca ccgtaccgct ctgtgacgta
    34981 ctatagtcgg caaacgtctc accttgaggt taaaaaatga gcaacatcct gattatcaac
    35041 ggcgcgaaaa aattcgccca ctccaatggt caactgaacg acaccctgac cgaagtcgcg
    35101 gatggcacac tgcgcgacct cgggcatgat gttcgcatcg ttcgcgccga cagcgactac
    35161 gatgtcaaag cggaagtaca aaactttctc tgggctgatg tggtgatctg gcagatgcca
    35221 ggctggtgga tgggcgcgcc gtggacagtg aaaaaataca ttgatgatgt attcaccgaa
    35281 ggtcacggga ctctgtatgc cagcgatggt cgtacccgca aagatccgtc gaaaaaatac
    35341 ggttccggcg gcctggtaca gggcaaaaaa tatatgcttt ctctgacctg gaacgcgcca
    35401 atggaagcct tcaccgaaaa agatcagttc ttccacggcg ttggcgttga cggtgtgtat
    35461 ctgccgttcc ataaagcaaa ccaattcctc ggtatggaac cgctgccgac atttatcgct
    35521 aatgacgtga taaaaatgcc tgatgttccc cgctatactg aagaatatcg caagcatctt
    35581 gtggaaattt ttggttaact agagctcagg ctttagaagg agttaaccat gcttaccgta
    35641 atcgcagaaa tccgtactcg tcctggtcaa catcaccgtc aggcggtatt ggatcagttt
    35701 gctaaaatcg ttccaaccgt actgaaagaa gaaggttgcc acggctatgc gccaatggtg
    35761 gattgcgctg ctggcgtgag tttccagtct atggcaccgg attctatcgt gatgattgag
    35821 cagtgggaaa gcatcgcgca tcttgaagcg catctgcaaa ccccgcacat gaaggcgtat
    35881 agcgaagccg taaaaggtga cgtgctggag atgaatattc gtattctgca accggggatt
    35941 taaacctgcc ttgtttgccc ggccatcctg accgggcaat gttctttcct ttaaacctca
    36001 atctccgcca tgtcgccttt ctcttgcaac caattgcggc gatcttccga gcgtttcttc
    36061 gccagcagca tatccatcat cgcgtcagta cgctgatcgt cttcatcatc gatagtcagc
    36121 tgcaccagac ggcgagtgtt cggatcaagc gtggtttcgc gcaattgcat cgggttcatt
    36181 tcccccagac ctttaaaacg ctggacgttc ggcttgcctt tcttgcgttt taattgctca
    36241 agtacgccct ctttctcttc ttccgtcagc gcgtaataaa cctctttccc gagatcaata
    36301 cggtagagcg gtggcagtgc gacgtaaacg tgaccgtgtt tcaccaacgc gcggaagtgt
    36361 tttacgaaca aagcgcagag cagcgtggca atgtgcagac catcagagtc cgcatccgcg
    36421 aggatacaga ttttgccata acgaagctgg ctcaggtcgt cgctgtcagg atcgataccg
    36481 atcgctaccg aaatatcatg cacttcctgc gaggccagca cttcgtcgga agaaacttcc
    36541 caggtgttaa ggatcttacc tttcagcggc atgatcgcct gatattcacg atcgcgcgcc
    36601 tgcttggcag atccgcctgc ggagtcacct tccacaagga acagttcggt acggttaaga
    36661 tcctgcgcgg tacagtcagc cagtttgcca ggcagcgccg ggccgctggt cagcttttta
    36721 cgcaccactt ttttagccgc acgcatacga cgctgggcgc tggaaatcgc catctctgcc
    36781 agcaactccg ccgcctgaac gttctggttc agccacagga tgaaggcatc tttcaccacg
    36841 cccgaaacga atgccgcaca ctgacgcgaa gagagacgct ctttggtctg cccggcaaac
    36901 tgcggatcct gcatttttac tgacagcaca taggcgcagc gatcccagat atcttccgcc
    36961 gacaacttta caccgcgcgg caaaatgttg cggtattcac agaactcacg catcgcgtcc
    37021 agcaggccct gacgcaggcc attaacatgg gtaccgccct gcatcgttgg gatcaggttg
    37081 acgtagcttt cggtcagcag ttcaccgcct tccggcagcc acagtagcgc ccagtccacc
    37141 gcttcagtat cgccagcgaa attaccgata aagggttttt ctggcagcgt cggtaaaccg
    37201 tttaccgctt ccgccaggta atcattcaga ccgtcctgat agcaccagcg ctgttcggtg
    37261 ttgttgatct catctttaaa ggtaatctca acgccggggc acagtactgc tttggctttc
    37321 agcacatgcg tcaggcgtga aacagaaaaa cgcgggctat cgaagaaggt ttcatccggc
    37381 cagaaatgga cgctggtgcc ggtattgcgt ttaccgcaag tgccgacaac ctgtaaatcc
    37441 tgcacctttt cgccattttc aaaggcgatg ttataaacct gaccatcgcg gcgcacgtta
    37501 acttctacgc gcttcgacag ggcgttaacc accgaaatcc ccacgccatg caggccgcca
    37561 gagaactggt aatttttgtt agagaattta ccgcctgcat gcagacggca aagaatcagt
    37621 tcaaccgccg gtacaccctc ttccgggtga atatccaccg gcatcccgcg cccatcgtca
    37681 ataacttcta acgactggtc agcatgtaaa ataacgtcca cgcgttttgc gtgacccgcc
    37741 agtgcttcat ccacactgtt atcaatgact tcttgcccca aatggttagg gcgagtggta
    37801 tcggtataca tccccggacg gcggcgaacc ggctcaagcc cggtgagtac ctcaatggca
    37861 tcagcgttat aagtttgcgt catggtttaa gttagcaatt cgagttgatc gtcagagatg
    37921 gtgcagacca agaaaatcga cgatcgggtt gaaataatct tcgaagcccg tgaatgcgtg
    37981 gttgccgcct tctatgacag tctggcggca ggaagcgtag tacgccaccg cctggcggta
    38041 atccagcact tcatctcccg tctgttgcag cagccagatc aaatccggcg cttccagcgg
    38101 gtcaatctgc atgactttaa gatcgtaaat atggcgtgac tctagcacat attgctgccc
    38161 ggtgtagggg ttctcgttct gaccgagata gtccgtcagc agttcaaacg ggcgcaccgc
    38221 cgggtttacc accactgcgg gcagcataaa acattgtgac aaccaggtgg cgtaatatcc
    38281 ccccagtgac gaaccgacaa tacccagcga atcgccgcca tgttccagga caatggattc
    38341 cagcagctct gccgcgtcgg aaggatacgg cggcaactgc ggaatgatca tctcaacgtc
    38401 agggtgatgt tccgccagcc agttttttaa caagctcgct tttgcagagc gcggcgagct
    38461 gttgaaaccg tgtaaataaa gaagcgtaga catcagtagc cttctgaagc ggtatcaggt
    38521 tggaaacgtg tgtccgccag gcgatgcacc tcggtggtca gcgtgccatc agcatgtaac
    38581 tcgagagtac gccagccggg cgcgatagta tccagcgtaa agttggaaca gtgcggctta
    38641 aactgcacac aggtcgacgg cgttgccagc aggcggcgac cgttccagtc gagatccagc
    38701 tcctgatgaa tatgaccgca cagcaagtat ttgacgtgcg gaaacttcgc cagcacggta
    38761 tccagttcgc ccgcgttacg caggctgtgt tgatcaagcc aactacaacc cgcaggtagc
    38821 ggatgatgat gcagcagcag caacgtatgg cgctctggcg catcggccag tttacgttcc
    38881 agccactcaa gctgaaactc gcttagctca ccgtgcggca cgccaaacac ctggctatcc
    38941 agcaacagga tttgccattg ctcaccaata aacacgcgct tcgccgggga gatacccgca
    39001 tcctgtaacg cgctgtacat cgcgggctgg aaatcgtggt tgcccggcag ccagacgcag
    39061 ggcgcacgaa aacttgcgat gccttcagcg aaatgctgat aggccgcaga ggattgatcc
    39121 tgcgctaaat cacctgtcgc gacaatcagg tcgaattcgt gctggtgtgg acgaatcgcc
    39181 tccagcaccg cctggtagct ttcccaggtg tttaccccta acagggcttc gtgcttttgt
    39241 gcaaacaggt gagtatcggt aatttgtaaa atcctgactc tggcctcacc agccagagga
    39301 agggttaaca ggctttccaa atggtgtcct taggtttcac gacgctagta aaccggaatc
    39361 gccatcgctc catgtgctaa acagtatcgc aaccagtccg ctaaaaactg gttaatttga
    39421 tgcttttcgt cgcgttgatg caacttttta ttaggataat cataccgcgc tttgaagcga
    39481 aaaatctgct ggcttgaaca cacttcagcc accatcgcgt catgatacag acgcaccgtc
    39541 attgacggaa ggctccagta actgatcgcg ggcgcagtct gttctattgt caccagggta
    39601 gtgtatcggg tcgattccac aatcgtcagc cgatattgtg cgtttgccac ctgatagctt
    39661 acagtttcgc cgggtgcgtc attgcgcggt aacaaacggc gcaattgtga aaagttcatc
    39721 tcgcacaggc gcatcatttc aggaaagtca ggtgtgtaac gcttcattta tacccactca
    39781 ttttttaacg cttgatgatg cagctgcagc cattgcaaag cgatgaccga cgctgcgttg
    39841 tcgattttcc cctcttctac ccactggtat gcctgttccc ggcttaccac atgaacgcga
    39901 atatcttcgt tttcatcagc cagaccgtga ataccgcttg cggtcgtggc gtccacttcg
    39961 cccaccataa ttgacgaacg ctcactggtg ccccccgggc ttgccaggaa acttaacacc
    40021 ggtttggtcc gtttgactat cagccccgcc tcttcaatcg cttcgcgacg ggcaacatct
    40081 tccacacttt caccctcttc tatcatcccg gcaaccatct ccagtagcca gggggtttcg
    40141 ctagtgtcgt acgcggcaat tcgaatctgc tcaatcagca caacttcatc acgcactggg
    40201 tcaaagggta gcaagactgc ggcgtgaccg cgctcaaaaa tttcccgccg tacctcatga
    40261 ctcatttgcc cgttgaatag acgatgacga aatctataaa gatctaatga aaaaaagccg
    40321 cgataaagtg tttctcgtgc aataatttct acatcgtttt tgccaaatgt aacgggcagg
    40381 ttgtctggct taagcattgt taatgtcctg gcactaatag tgaattaaat gtgaatttca
    40441 gcgacgtttg actgccgttt gagcagtcat gtgttaaatt gaggcacatt aacgccctat
    40501 ggcacgtaac gccaaccttt tgcggtagcg gcttctgcta gaatccgcaa taattttaca
    40561 gtttgatcgc gctaaatact gcttcaccac aaggaatgca aatgaagaaa ttgctcccca
    40621 ttcttatcgg cctgagcctt tctgggttca gttcgttgag ccaggccgag aacctgatgc
    40681 aagtttatca gcaagcacgc cttagtaacc cggaattgcg taagtctgcc gccgatcgtg
    40741 atgctgcctt tgaaaaaatt aatgaagcgc gcagtccatt actgccacag ctaggtttag
    40801 gtgcagatta cacctatagc aacggctacc gcgacgcgaa cggcatcaac tctaacgcga
    40861 ccagtgcgtc cctgcagtta actcaatcca tttttgatat gtcgaaatgg cgtgcgttaa
    40921 cgctgcagga aaaagcagca gggattcagg acgtcacata tcagaccgat cagcaaacct
    40981 tgatcctcaa caccgcgacc gcttatttca acgtgttgaa tgctattgac gttctttcct
    41041 atacacaggc gcaaaaagaa gcgatctacc gtcaattaga tcaaaccacc caacgtttta
    41101 acgtgggcct ggtagcgatc accgacgtgc agaacgcccg cgcgcagtac gataccgtgc
    41161 tggcgaacga agtgaccgca cgtaataacc ttgataacgc ggtagagcag ctgcgccaga
    41221 tcaccggtaa ctactatccg gaactggcgg cgctgaatgt cgaaaacttt aaaaccgaca
    41281 aaccacagcc ggttaacgcg ctgctgaaag aagccgaaaa acgcaacctg tcgctgttac
    41341 aggcacgctt gagccaggac ctggcgcgcg agcaaattcg ccaggcgcag gatggtcact
    41401 taccgactct ggatttaacg gcttctaccg ggatttctga cacctcttat agcggttcga
    41461 aaacccgtgg tgccgctggt acccagtatg acgatagcaa tatgggccag aacaaagttg
    41521 gcctgagctt ctcgctgccg atttatcagg gcggaatggt taactcgcag gtgaaacagg
    41581 cccagtacaa ctttgttggt gccagcgagc aactggaaag cgcgcatcgt agcgtcgtgc
    41641 aaaccgtacg ttcctccttc aacaacatta atgcttctat cagtagtatt aacgcctaca
    41701 aacaagccgt ggtttccgcc caaagctcat tagacgcgat ggaagcgggc tactcggtcg
    41761 gtacgcgtac cattgttgat gtgttggatg cgaccaccac gctgtacaac gccaagcaag
    41821 agctggcgaa tgcgcgttat aactacctga ttaatcagct gaatattaag tcagccctgg
    41881 gtacattgaa cgagcaggat ctgctggcac tgaacaatgc gctgagcaaa ccggtttcca
    41941 ctaatccgga aaacgttgcc ccgcaaacgc cggaacagaa tgctattgct gatggttatg
    42001 cgcctgatag ccctgcaccc gtcgttcagc aaacatccgc acgcactacc accagtaacg
    42061 gtcataaccc tttccgtaac tgatgacgac gacggggctt cggccccgtc tgaacgtaag
    42121 gcaacgtaaa gatacgggtt atctgccgca ttcttccccc ttctcgcttc aatttcgacc
    42181 agccatcctc tattctgatg ggtatttacc actggtcccg gaagacaaaa atgaaacgga
    42241 caaaatccat acgccacgca tcgttccgca aaaactggag cgcacgccat ctgacaccag
    42301 tcgctctcgc agttgccact gtttttatgc tggctggctg tgaaaagagt gatgaaacag
    42361 tgtctctcta tcaaaatgct gacgactgtt cagctgcaaa cccaggcaaa agcgccgaat
    42421 gtaccaccgc gtacaacaat gcgctgaaag aagccgaacg tactgcgccg aaatacgcca
    42481 cccgtgaaga ctgtgttgct gaatttggtg aaggtcagtg ccagcaggca ccagcccagg
    42541 ctggcatggc accagaaaac caggcgcagg cccagcaatc cagcgggagt ttctggatgc
    42601 cgctgatggc cggttacatg atgggtcgtc tgatgggcgg cggcgcggga tttgcacagc
    42661 agccgctgtt ctcctcgaaa aacccagcca gcccggctta cggtaaatat accgacgcga
    42721 cgggtaaaaa ctatggcgca gcccagccag gccgcaccat gaccgtaccg aagacagcca
    42781 tggcaccaaa accggcgacc accactaccg ttacccgtgg cggttttggt gaatctgttg
    42841 ccaaacaaag cactatgcag cgtagcgcaa ccggtacctc ttctcgttca atgggtggct
    42901 gataccgatg gaaagagtca gtattaccga gcgcccggac tggcgtgaga aagcccacga
    42961 atacggtttc aattttcaca ccatgtacgg cgagccgtac tggtgtgaag atgcttacta
    43021 caagttgacc ctcgcccagg ttgaaaagct ggaagaagtc accgccgaac tgcaccagat
    43081 gtgcctgaaa gtggtggaaa aagtgatcgc cagcgatgag ctgatgacca aattccgcat
    43141 tccaaaacac acctggagtt ttgtgcgcca gtcatggctg acgcaccagc catcgcttta
    43201 ttcgcgtctt gatctggcgt gggatggcac tggcgaacct aaacttctgg aaaataacgc
    43261 cgatacgcca acgtcactat acgaggcggc gttctttcag tggatctggc tggaagatca
    43321 gcttaacgcc ggtaacttgc cggagggcag cgaccagttt aacagtctgc aagaaaaact
    43381 gatcgatcgc ttcgttgagc tgcgtgaaca gtatggcttc cagttgctgc atctcacctg
    43441 ctgtcgcgac acggtggaag atcgcggtac cattcagtat ttgcaggact gcgcgacgga
    43501 agctgaaatc gctaccgagt tcctctacat tgacgatatc gggttaggtg aaaaaggtca
    43561 gttcacggat ttacaggatc aggtaatttc caacctgttc aagctgtatc cgtgggaatt
    43621 tatgttgcgt gagatgtttt ccaccaagct ggaggatgca ggcgtacgct ggctggaacc
    43681 ggcgtggaag agcattatct ccaacaaggc acttctaccg ctactgtggg agatgttccc
    43741 gaatcacccg aacctgctgc ccgcttattt tgcggaagat gatcatccgc aaatggaaaa
    43801 atatgtggtt aaaccgatct tctcccgtga aggcgcaaac gtgtcgatca ttgagaacgg
    43861 caaaaccatt gaagcagcgg aaggtccgta tggcgaagaa gggatgattg ttcagcaatt
    43921 ccacccgtta ccgaaattcg gcgacagcta tatgctgatt ggtagctggc tggtgaacga
    43981 tcaacccgcc ggaattggca ttcgtgaaga ccgtgcattg atcacccagg atatgtctcg
    44041 gttttatcca catatttttg ttgaataatc aatgatgccg gatggcattc gccatccggt
    44101 aattgttagc ctatctgcac cgacagcata cttaaactgc ccatttctat accatcgacc
    44161 ggaatggtaa ttggctcctg cccatcccac gcacctaaca catacaacaa cggcagatag
    44221 tgctctggcg ttgggttcga taacgcgcca ccttcatggt cgagataatt caccagagga
    44281 tgctgctcta ctggcccttg ccacgtcaga ttcgctttca catactcatt aaacgacgtc
    44341 gcccacggat acggtgaact atcaccgtgc cacttcactg tacgcaggtt atgcaccacg
    44401 ttgccgctgg cgaccaacat tattccttca tcacgcagcg ccgcgagttt tcgccccatt
    44461 tcgaaatgcc aggcggcagg tttgctacta tcgatactca actgcaccat cgggatatcg
    44521 gcgtcaggat acatcttaat cagcacgccc caggaaccgt ggtcaaagcc ccaggcttct
    44581 ttatccagcg tcaccgggac cggcgctaac agctcaacca gatgctgtgc cagcgcaggc
    44641 gaacccggag caggataatg cgtatcgtac agcgcctgcg ggaagccacc aaagtcatga
    44701 atcgtgggcg gcgtctccat cgcggtcact cctgttccac gggtaaacca gtgagccgaa
    44761 actaccacaa tcgcttgcgg gcgtggcaat gtcatcccca acgtctgcca gctgcgggta
    44821 tacaaattat cttccagcac gttcatcgga ctaccgtgac ctaaaaacaa tgctggcata
    44881 cgtgttgaag acatgatgat atccttaact aaaggtgtca ttttgatatc ctcacaatac
    44941 gcttgttcgg cggagtaaga acccggataa caatgatgat gatcatcagt tattttgacg
    45001 atctgcctga aggtgaagat ttataaggag ttgtcgatgt cagtacctct cattctgacc
    45061 atactggcgg gggcagccac gtttattggc gcgtttctcg gcgttctcgg gcaaaaaccc
    45121 tcgaaccgct tactggcgtt ttcgctagga tttgcggcgg ggatcatgtt gctcatctca
    45181 ttaatggaaa tgcttcctgc cgcactagca gctgaaggaa tgtcgcctgt gttgggttat
    45241 ggaatgttta tcttcggtct gcttggctat tttggcctgg accgcatgtt gccacatgcg
    45301 catccgcagg atttaatgca aaaatcggtg cagccgttgc caaaatcgat caagcgcaca
    45361 gccattctgc tcactctcgg catcagtctg cataacttcc cggaagggat tgccaccttt
    45421 gtcacggcga gcagcaacct ggagctggga tttggcatcg ctctggccgt agcgttgcac
    45481 aatattcctg aaggtctggc agtggcaggc ccggtttacg cagcaacggg gtctaaacgc
    45541 acggcgattc tgtgggcggg gatttctgga ctagcagaaa ttcttggtgg cgtgctggcg
    45601 tggttaatcc tcggtagcat gatttccccg gtggtcatgg cagcaatcat ggcggcggtc
    45661 gcaggaatta tggtggcgct ctcggttgat gaattaatgc cgctcgccaa agagattgac
    45721 cctaataata accccagcta tggcgtacta tgtggaatgt cagtgatggg attcagtttg
    45781 gtgctgctac aaacggcggg aattggttaa aaacaacatc agaagttgtc cagcgcatgg
    45841 acaacttctg ctagtttcac taaagcctta ttctaccttt tcggctgtct cttctctcgt
    45901 actgtttaag tatttgttcc gcgcttcaag cagtgcgtct ctgtcctggc gaaatgcccg
    45961 acaataacgt ttcaaatgca ggaaataacc caccacaacc agaacaatca atacaatcca
    46021 gtaccaggcg ataaacatcc ttttaccctt aatatttaac aatactaaac atttagcgca
    46081 taaatttcac atatcctttt tcggatatat tcatatggtc gcataataac ggaacaatta
    46141 tatgattttg atcataatga aaaagaatgc ctgatattaa tgaaattttc tcatagaaag
    46201 agaagcggaa ttaacatctt gcatagcagg gaaaaataaa aaccgaagcc tgctacaact
    46261 tcggtttctt gattaaggca gtaaattaag cagcggtttt cagctggctt tacgctcatg
    46321 tgcctgacgg tatgccacca ggtcttcaat agtcacaagc gccatattgt gtttattggc
    46381 aaactcaatg cactctggtg cacgcgccat cgtgccatcg tcattagtca gctcacacag
    46441 cacaccagcc ggtttgaagc ctgccagcgt cattagatca atagttgctt cagtatgacc
    46501 gccacgcgtc agtacaccac cagcctgagc gcggagtggg aaaacgtggc ccgggcgatt
    46561 cagatcagaa ggttttgcgc catcggcaat cgctgcgcga acggtcgtaa tacggtcagc
    46621 ggcagaaaca ccggtagtca caccttcagc cgcttcaatg gtcacggtaa aaccggtgcc
    46681 ataggcgctg gtgttatttt ctaccatcat tggcagatcg agttgtttac ggcgatcttc
    46741 agtaatgcac aggcaaacaa taccactacc gtggcgaatg gtcagcgcca tctgctcaac
    46801 agtcatggtt tctgccggga agatcatatc accttcgttt tcacggtctt catcatcaag
    46861 caccattaca ccgcgtcctt cacgcagcgc agccagtgca ttttcaacac gttcgaaagg
    46921 cgtaccaaaa gaggaaagta gcgtctgatt catggtaaaa aaacctcact aaaattatgg
    46981 ttaccagaat cagggcagtc ttaggagtgg cggcatatag ccaaaataac gtgagcgggt
    47041 ccatgcccga cagaatcgtt actctctccc atccggactc taaccgtcgg ccccggaatt
    47101 acaccggatc tgctgtcctt tgagttcgca cccaaagcgc tcgcgggctt tcaactgagt
    47161 tgatttaccg ccggtgggga atttcgcccc gccctgagaa taagcggatt cactataacg
    47221 ctaatgatta gcggcagcaa cgcatagctt cacataattc tggtttatga cttaccctta
    47281 tcgcactaca atggcactca acacctatca gtacagggaa actacaatga ttgacccgaa
    47341 aaaaattgag caaatcgctc gccaggttca cgaatcaatg cctaaaggaa tcagggagtt
    47401 cggggaagat gtggagaaaa aaatccgcca aaccctacaa gcgcagctga cgcgtctcga
    47461 tctggtaagc cgcgaagaat tcgacgttca gacgcaagtc ctgttgcgga ctcgtgaaaa
    47521 actggcgtta ctggagcagc gcatcagtga actggaagcg cgcaataatt cggtagcaga
    47581 tcttcaatct cctccggcta tcccgcctat tgataaagca gaataaagca acacaaaaac
    47641 gggccagacg gcccgtaata tcagtgttct aattcaagtt ctctggtttg catcatctgt
    47701 tggcaatacc aggcataacg tgcgcaaaac caggttctat gttcttcatc catgttacca
    47761 gtaacggcca gaatatcgac cgggcgacct tctgcgtgca ttctggcaaa actacgcgcc
    47821 aggaaatcga aattatttaa agaattaaga ctatgatcca taagcacatc ctccggttat
    47881 cgcatacctt ttaaggccat atgctttttc tgatttcagt attccccaat cagtgtagtg
    47941 cctttaaaca taacgtgcgt aaatatattg tccccgatca cactttttag tgaaaaataa
    48001 aaaaccatat taaaagactt tctcttccag aacttctcta acatgtgtta ttattaacaa
    48061 agacaaatgt aagcaaggaa actatcccaa agtaaatatt gcgtcactaa atggacattg
    48121 gagtgacata tgattttatt cgccgactat aacacccctt atcttttcgc tatttccttt
    48181 gtactgttga tcggcctttt agaaattttt gcgcttatct gcggtcacat gctctccggt
    48241 gctcttgatg cacatcttga ccattatgat tccataacca caggtcatat tagccaggca
    48301 cttcattatc tcaatatcgg aagattgcca gctctcgtcg ttctctgctt gctggctggt
    48361 ttttttggtc ttattggcat cctgttgcag catgcctgca tcatggtctg gcaatcacca
    48421 ctgtcgaacc tgttcgtggt tcctgtcagt ttactgttta cgattattgc ggtgcattat
    48481 accggtaaaa tagttgcgcc ctggattcca cgcgaccata gttcagctat tacagaagaa
    48541 gaatatattg gtagcatggc attaattacc ggtcatcaag ccacttcagg taatccctgc
    48601 gaaggaaaac tcaccgatca atttggccaa atacattatt tactactgga gcctgaagaa
    48661 ggaaaaattt tcactaaagg agacaaagtg ttaattattt gccgactttc tgcaacgcga
    48721 tatttggcgg aaaacaatcc ctggccccaa atattgtaat ttattaatca aaaggaaatt
    48781 taataatgga tgatattgtt aattctgtgc cctcctggat gtttaccgcg attattgccg
    48841 tatgcattct gtttattatt ggaattattt tcgccaggct ctatcgtcgc gcttcggcag
    48901 agcaagcttt tgttcgtact ggtttaggtg ggcaaaaagt ggtaatgagc ggtggcgcaa
    48961 tcgtgatgcc gatctttcat gaaataatcc ccatcaatat gaatactctg aagctggaag
    49021 tcagccgctc aaccattgat agcctgatta cgaaagatcg tatgcgcgtc gatgtagtag
    49081 tcgctttctt tgtgcgggta aaaccttcag tagaagggat tgccaccgct gcccagacgc
    49141 tggggcaacg caccctgtcg cctgaagact tacgtatgtt ggttgaagat aaatttgtcg
    49201 atgccctccg tgcaacagct gcgcaaatga ccatgcatga gttacaggat acccgcgaga
    49261 acttcgtgca gggagtacaa aataccgtag ctgaagatct gtcgaaaaac ggcctggaac
    49321 tggaaagcgt ttcacttacc aactttaacc agacctcgaa agaacatttc aatccgaaca
    49381 atgcctttga cgccgaaggt ttaaccaaac tgactcagga gacggagcgc cgtcgccgcg
    49441 agcgtaacga agttgaacag gatgtagaag ttgcggtgcg tgagaaaaac cgtgatgcac
    49501 tttcgcgcaa gttggagatt gaacaacaag aagcgtttat gacgcttgag caggagcagc
    49561 aggttaaaac ccgtaccgct gagcagaatg cgaaaattgc ggcttttgaa gctgaacgtc
    49621 gtcgtgaagc agagcagacg cgaattctgg ctgaacgaca gattcaggaa acagaaatcg
    49681 accgcgaaca ggccgtccgc tcaagaaaag ttgaagctga acgtgaagtt cgcattaaag
    49741 agatcgaaca gcagcaggtc accgaaatcg ctaaccagac gaaatcgatc gctattgccg
    49801 ccaaatcgga acaacagtcc caggcagaag cgcgtgctaa ccttgcactc gcagaagcag
    49861 taagcgccca acaaaacgtt gaaaccactc gccagaccgc agaagccgat cgtgctaaac
    49921 aagttgccct aatcgctgcc gcgcaggatg cagaaaccaa agcggttgaa ctgaccgtgc
    49981 gggcaaaagc agaaaaagaa gccgcagaga tgcaggcggc ggctatcgtt gagttagccg
    50041 aagctacacg taaaaagggt ctggcggaag cagaagcaca acgtgcgctg aacgatgcta
    50101 tcaacgtact ttctgatgaa caaaccagcc ttaaattcaa actggccttg ttgcaggcgc
    50161 tgcctgcggt aatagaaaaa tccgttgagc cgatgaaatc cattgacggt atcaagatta
    50221 ttcaggtcga tggtctgaat cgtggcagcg ctgcgggtga tgcaaacacg ggtaatgtgg
    50281 ggggcggaaa cctggcggaa caagcgttat cagccgcact ctcttaccgc acacaggcac
    50341 cgctgattga ctccttgctc aatgaaattg gcgtttcagg cggctcactg gcggcattga
    50401 cttcaccctt aacctcaaca actcccgtcg cagaaaacgt agaataaatt ttcaactatg
    50461 aacatccctg gcccaaaaag ccggggattt tttatatctg cgttccgcta aaaactgcaa
    50521 atgatcaggt tgttgcaacg ttttgcgcga ccgctcgggg aaggcaaaac tgccacaggg
    50581 aaagcattgc gcgaggtccg gcgctcatca acaatcggga agtcagcaag gggctgaaac
    50641 gggaaagccc ctcccgaaga aggggcctta cagaaggaaa gggttatgat gaagctcgtc
    50701 atcatactga ttgtgttgtt actcgtaagt ttcgcagctt attaacagcc aatcagaggg
    50761 gggagaaatc ctccctcacc cttgttcctt tactttaggt tgaaaaaaaa tcagcgtcaa
    50821 gaaggtttca ttaacacata tccctggccc aaaaagccgg ggatttttta tatctgcgtt
    50881 ccgctaaaag gtgcaaatgc tcaggacgtt gcagcgtttt gcgtgaccgc tcggggaagg
    50941 caaaactgct tcagggaaag cgttgcgcgg ggtccggcgc tcatcaacaa tcggggagac
    51001 agcaaggggc tgaaacggga aagcccctcc cgaggaaggg gccttataga aggaaagggt
    51061 tatgatgaag ctcgtcatca tactggttgt gttgttactg ttaagtttcc cgacttacta
    51121 acaactcatc agagggggga gaaatcctcc cttacccttg ttcctttact ctaggttgaa
    51181 aaaacaacag cgtcaatagg cctgccatgt acgaagcgag atctgtgaac cgctttccgg
    51241 ttagcctttt ttatcctgtt ggatcttctt gataatgtta gtggtcgagc aaccgtcttc
    51301 aaagttgagc accaacactt cgccgccgtt ggcccagact tctttactcc cggcgatctc
    51361 ttccggtttg tagtcaccgc ctttcaccag caggtctggc aggatcccgg cgatcagacg
    51421 ctgcggcgtg tcttcttcaa acgacactac ccagtcgacc gcttccagtg cgcccagcac
    51481 aatcatacgc tgttcgagtg ggtttaccgg gcgggaatcc cctttcagcc gtttggtgga
    51541 ggcatcgctg ttgacggcaa caatcaagcg gtcacccagc ttgcgggcat ttgccagata
    51601 agagacgtgc ccggcatgta ggatatcgaa gacaccgttg gtcattacca ctttttcacc
    51661 acgtttacgc gctgccgcta cagccagctt cagttcctct tcggtcatca cgccaaagcc
    51721 ggtatctgcg cgtccacgta cagcgttttc cagctcgatc ggcgaaaccg tggaggttcc
    51781 cagtttgccg accaccacgc cagccgccgc attggcaaag aagcaggctt cttccagcga
    51841 attacccgct gccagcgttg ccgccaggac gccaatcacc gtgtcgcccg caccggtaac
    51901 gtcatacact tcctgcgctt gggttggcat atgcagcggc gctttacccg gttgcagcag
    51961 cgacataccc tgttcggaac gggtcactaa cagagccgag agttcgtaat cggcaatcag
    52021 tttcatgccg cgctcaacaa tctcttcttc ggtcttacat ttaccgacaa cagcttcaaa
    52081 ttccgagaga ttcggcgtta acagcgtagc gccgcggtag cgctcaaaat cggtaccttt
    52141 tggatcaatc agcaccggaa cacccgcttt acgcgcctgt tggatcatct gctgtacgct
    52201 tgccagcgca cctttggcgt agtcagaaag caccagcgcg ccaatcgaac tcagcgcctg
    52261 attaatccgt tcgtgcagcg gttgcggatc aacaccttcg aaaccttctt caaaatccag
    52321 gcggatcagc tgttggttgc gggaaagtac ccgtaatttg gtgatggtcg gatgcgtcgg
    52381 tacagaaacg aagtcgcatt tgacgttgac gtcggccaga gatttactca gcgcgcgcgc
    52441 tgcatcgtca atgcccgtca acccgaccag gcgtgcatta gcaccgagag aagcgatatt
    52501 catcgccacg ttagccgcgc cgcccggacg ttcttcgatg gtattcactt taaccacggg
    52561 caccggcgct tccggcgaga tacgactggt ggggccgtac cagtaacgat ccagcatcac
    52621 atcaccaacc accatcactc ctgcacgttc aaactctggc agcgttactt tcattcctgt
    52681 ctcctgagag attcaaaatt tgcgcgcgat aataccatac ttcattcttc caccagccac
    52741 ttctgccagc ttgcccgcac caaatcacgc tctgcggtga agcaatcctc cgacacatgg
    52801 cccggcaatt cctgtaatgc cagatgatga agttcatcgc gcagcgtcgt gtaagcacgg
    52861 gtcagcgcca tcgcttcctg ctcttccata atgtcgtttt gcgccagcaa ttccagaatg
    52921 cgcacgttgt ctgaccagcg cgttaacttc ggtttttcat gagcgtagcg caacaccaga
    52981 tattgagtaa taaattcgat atcggtaatt ccgccttcat cggctttgat atcaaagcga
    53041 tcgcgatgtt tattgccgag atgagcgcgc attttttcgc gcatttcccg cacttccgtt
    53101 tgcagagttt taccttcacg cggcagcgtc ataatctcgc ggcgcaccgc gtcaaagtgc
    53161 gcggtgagct gcggatcgcc gtacactaca cgcgcacgca ccagcgcctg atgttcccac
    53221 gtccaggcct cgtttttctg ataatcagca aatgcttctg cggatgtcac cagcattccc
    53281 gccgccccgg acgggcgtag ccgtgcatcc acttcataca aaatgccgga agaagtacgc
    53341 gtgctgaaca gatgcataat gcgttgcgcc agacgcagat aaaactgccg cccgtcgatt
    53401 tcccgctcgc cgtccgtcat tacatccatc gggcaatcat ggagaaagat caggtcaaga
    53461 tcagaactgt agcctaactc ccagccgccc agcttgccgt agccgaccac cgcaaagccg
    53521 cgaccttcac gttcattcag gtgagtcggt tttccgtaac gggcaaccat ttgaacccac
    53581 gcttgctgga ccaccgcatc tatcattgcc tccgccagcc aggttaagtg atcgctcact
    53641 ttcattaccg gcagcgtacc ggcgatatcc gctgccgcaa tgcgtaacag ttgcgcctgt
    53701 ttgaactgac gcagcgcctc aagctgttgt tcttcgtcat cttccggcac gcgcagcaaa
    53761 tactggcgca actcatcacg gtaagcatcg gtcgccgtcg gctggtagag agtgtttgga
    53821 tcgagcaatt catccagcaa taatggataa cgcgccagct ggctggcaat catcggcgac
    53881 gcggcacaca gagaaatcaa atgtttaagc gccgcgggga attcactgag caattctaaa
    53941 taggtggtgc gggtaacaat ccccaccagc aaggcggtaa tgcgcgacag cgtgacggca
    54001 gcgtcttcac gcgcacagac atcacttagc agatgtggca tcagatggtc gagcacctga
    54061 cgtcctcgcg gcccgatggt gcgcttatcc agctctttgc ggaaatcagc aatcagcgtt
    54121 agcacctgtt tgcgatcatt ttcgctaaga tgcgccagca ctggcgtggt gtcatcttcc
    54181 tgcaacgcat cctgccacag ctcacgccac tgttccgaca gcgactcttc ctgagtttca
    54241 ctttcatcgt cgccaatcaa ttcattaaac acccggcgca cattggtcat atgtgcggtc
    54301 agcgccacgg tcagttgcgg ccagtcagca aagtccatcg cccacgccag ccgcgcacgg
    54361 ttaagctcat cagaaggaag cgtctgggtt tgttcgtcgt taatgctttg cagcaggttt
    54421 tccagacgcc gcaggaacag atacgccact cgcaattgtt cagcatcgtt ttcagaaagc
    54481 agatgcagcg cggcaatgac gctgagcgtt ggcagtaaag agcgcgactg cagcgacggt
    54541 tcgcgcccac cgcgaatgag ctggaacacc tgaacgataa attcaatttc gcgaatgccg
    54601 cctgcgccga gtttgatatt gtcggtcaaa ccacgtcgac gcacttcacg ggcaatcatc
    54661 cctttcatgt tgcgcagcga ctgaatcacg ctgaaatcga tgtaacgacg gaaaacaaac
    54721 gggcgcagca tcgcacgcaa ctcgttagca tagacgcctt cgctatcgcc cataatccgc
    54781 gccttgacca tcgcgtaacg ctcccagtcg cgcccctgct cctggtaata atcttccagc
    54841 gccgcaaagc tcagcaccag cgggccactt tcgccaaacg gacgcagccg catatccacg
    54901 cgatagacga agccatcctg cgttggttga tccagcactt taatcagccg ctgccccatg
    54961 cgggtaaaaa actgggcgtt atccagttcc cggcgtccac cctgcgtaca accatgttcc
    55021 ggccaggcaa aaatcagatc gatatcagag gagaaattca gttccccgcc gcccagctta
    55081 cccatgccta aaatcagcag cggttgcgct tcgccctgcg cattgcacgg cgttccccac
    55141 tcgcggcagc aggcgtcata tagccagtca cgcgccgcaa caatcagcgt ctccgccaga
    55201 tagctgagct gctgcaatat gctctcttca gtaaccagtg ccagcgtttg cgcccaggcg
    55261 atacgtacca tgatgcgccg acggaacagc cgcagctcgc gcattaaccc ggcttcgtca
    55321 ctcacattac tgagcgcctc ctgcaaccat gcaacgtaat gctgccattc gtcggcctgc
    55381 ggcggttggc tttccagttc cgtcagccac tctggatgcg caatcacgct gtcctgcaca
    55441 aaatcactga aagtaagtac tgacttcgcc tgtgcgctaa gtgattcctc ggctaaaggc
    55501 tctggcagcc gctcaacaac ggtctgccag tactgctgta acggtgaaga gagcggcttc
    55561 ataagcgatt ttatccttgc ctgattaacg ttttccgctg tgcaaccaga acggttcctg
    55621 attgtttgcc tcattacgga aatgttcaat ttcgatgcgt tgcccggtcg caatagcgtg
    55681 atgcagcccc tgccagttct ccagccaggc ttgcgcgacg acaggatcat agtaacccgc
    55741 cagcaacagt attgagtcaa tatcacgcgt caggcgtggc aactggtcac ggtagcgatc
    55801 gcctaacggc tggcagaaaa cgcttttcag ttcagcggca tggcgggaaa gatggatatc
    55861 ggcaaagcgt ttgaaggagt cgctgatttt gccctgcgct ttggcatcta aaaatggctg
    55921 ccatgctttg cttaccaacc attcggtcaa cgccagcttc gccattgccg tttcggtaga
    55981 gtagaccgcc gtcacggcag aaaccgcaga agcaatggtc gcttcgcatt gggtcagcaa
    56041 atcacgtaag tgagtgctcg ctttacgcgg cacaatacca ccgaacagca tcagcgtatg
    56101 acggaccagg ctaatggctg ccagcacctg ttctttcgcc gcatcgttgc cgcgtaccca
    56161 cagttcttca tgatactgcc attgcgctaa cgccagctcg agcgccgctt ccagcccctg
    56221 ttccacatcg gcttttgccg caacatgcaa aatggtggtc ggtttgattt cacgcgccgg
    56281 attgccctgc gccagatgat agccacgcgc cgctttgctc aggctgccct ggcgtaatcc
    56341 ggtttgcgat accagttggt tcgccagttt cagcaccgcg cgcgtgtcgc cgctaagcag
    56401 ttccagttcc agctcacaga taggttcagc aaattcctcc gctttcacct ccccctgatc
    56461 gagagcgatt tcaattcggc tgccatcgac cgccaccagc catttttcgc gataaaaatc
    56521 ggtgctgaac agcggttgta cgcgggaagc gagatcggca ggcaattcgc cgttcggcca
    56581 gacttccgag ggaaactgcg ccaggtcgag cgtcggttcg ctcaacgcca cgttatattc
    56641 cgggcgctgg tgtaagccgc ctgtcactct tcctgcaact ttcatggtca tctcatagcg
    56701 accgttttcg ccacgaatac gtaagcccat atcgtgccca cgcagccagt tatccggcgt
    56761 ttcgtagtaa atattcagca actgcacggg gtcatggtgc tcgccgccca gcgtattgag
    56821 atggtcacgc aacgcctcaa cggcactgtg attaacaata aactttaatt cgatttcctg
    56881 agccatggcc tttacttatc ggttatgtca cataagtgac gatgaacggc gaacttaatg
    56941 cgatcttttt tgtcagtaga tagtattttg cgccaaattg ccatgcaacg ggcaatttga
    57001 cgggcgtaaa agtttgaagc agtggcaaga cctgacacag aggattccga ttgatgtcga
    57061 atgctttgcg tagcgacact gatgccacta ctatcgttcc actttccatg acaataacga
    57121 cagcctgatg ccaaaattac gcctgatcgg attaacttta ctcgcactta gcgcgactgc
    57181 cgtctcacac gctgaagaaa cgcgctatgt ttctgacgaa ctgaatacct gggtccgtag
    57241 cggtccggga gatcattatc gcctcgtggg cacggttaac gccggcgagg aagtgacctt
    57301 attacaaact gacgccaaca ccaattatgc ccaggtgaaa gacagctctg gccgtaccgc
    57361 ctggatcccg ttgaaacaac ttagcactga gccaagcctg cgctcccgtg tgccagatct
    57421 ggaaaatcag gtcaaaaccc tgaccgataa actcaccaat atcgataaca cctggaatca
    57481 gcgcaccgca gaaatgcagc aaaaagtggc gcagagcgac agcgtgatca acgggttaaa
    57541 agaagaaaat cagaaactga aaaacgagct gattgtcgcg cagaaaaagg tcgatgccgc
    57601 cagcgtacag ctggatgaca aacagcgcac catcatcatg caatggttta tgtatggcgg
    57661 tggcgtgctg gggcttggct tgctgctcgg tctggtactg ccgcacctga tcccaagccg
    57721 caaacgcaaa gatcgctgga tgaactaaat cgccttcttc gttgcactgt cttattatta
    57781 ggaattgtgt atttaacgaa gggatgatgg cgtgaagatt tatctggtcg gtggtgctgt
    57841 tcgggatgca ttgttagggc taccggtcaa agacagagat tgggtggtgg tcggcagtac
    57901 gccacaggag atgctcgacg cgggctacca gcaggtaggc cgcgattttc ctgtgtttct
    57961 gcatccgcaa acgcatgaag agtatgcgct ggcacgtacc gaacggaaat ccggttccgg
    58021 ttacaccggt tttacctgct atgccgcacc ggatgtcacg ctggaagatg atcttaagcg
    58081 tcgcgatctg accattaatg cgctggccca ggacgataac ggcgagatta tcgacccgta
    58141 caacggtctg ggcgatctgc aaaatcgtct gttgcgccat gtttcccccg cttttggcga
    58201 agatccgtta cgcgtactgc gcgtggcgcg ttttgctgcg cgttatgccc acctcggttt
    58261 tcgtattgcc gatgaaactc tggcgttgat gcgcgagatg acccatgcgg gtgaactgga
    58321 acacctgacg cctgaacggg tgtggaaaga gacggaaagc gccctcacca cccgcaatcc
    58381 ccaggtgttc ttccaggtac tgcgcgattg cggtgcactt cgcgttttat tcccggaaat
    58441 tgacgccctg tttggcgttc cggcccctgc caggtggcat ccggaaatcg acacgggtat
    58501 tcatacctta atgacgctct caatggcggc gatgctgagt ccgcaggtcg atgtccgttt
    58561 cgcaacttta tgccacgatc tcggtaaagg actgacgccg ccagaactct ggccgcgtca
    58621 tcatggtcat ggcccggcgg gtgttaagtt agtggaacaa ctatgccagc gtctgcgcgt
    58681 gccaaatgaa attcgcgatt tagccagact ggtggctgag tttcacgatc tcatccacac
    58741 cttcccaatg ctgaacccaa aaaccatcgt caaattattt gattccatcg atgcctggcg
    58801 taaaccgcag cgtgtcgagc aactggcgct gaccagcgag gctgacgtgc gcggcagaac
    58861 cggttttgaa tcagcggact acccgcaagg ccgttggttg cgcgaagcct gggaagtggc
    58921 gcagtcagtg ccgacaaaag ccgtcgttga agcgggattt aaaggcgtgg agattcgcga
    58981 ggagctgacc cgacggcgga ttgcggcggt agccagctgg aaggaacaac gttgcccaaa
    59041 gcctgaatga gtattgggca ttttgccgga tgcggcgtaa acgccttatc cggctacatg
    59101 tcaacgacag ttgtaggcct gataagacgc gcaagcgtcg catcaggcgt tgattgccgg
    59161 atgcggcgtg aacgccttat tcggcctacg caatcgtgga caacgccagg catccaacac
    59221 tattcctcaa tcaaaagaac acgacataca ccgcagccgc cacaataaag cgataaatgg
    59281 cgaacgggat aaacgaaatg cgcttaatca attgcaggaa ggttttaatc gctatcagcg
    59341 ccaccacaaa agcggtgata aacccaacgg caaacatcgg gatatcgccg gttgtcagga
    59401 agccccagct tttgtagaga tcgagcgccg ttgcgcccat catcatcggc accgccagca
    59461 ggaacgaaaa ctcggaagca gcgtaacggc tcacccccat cagcatccca cctgaaatgg
    59521 tcgccccgga acgggagaaa cccggccaca gcgccagaca ctggaaacag ccaatcataa
    59581 atgcctgacg ataggtcata tcatcaagac ccggcgcacg cggctctttc ggcttcaggc
    59641 attcggcggc aatcagcaac aaaccgccaa cgaccagcgc atacatcaca tttatcgggt
    59701 taaacaatga cttaatcgtg tcgtggaaca acagccccag taccaccgcc ggaatcatcc
    59761 ccagcaaaat gtggatcagc gttaaacgac ctttgctttc tccttcgtgc tgcaacgggc
    59821 ggccaaagtg gatgccaatc aggccaaaca gacgccgcca gaacatcact actaccgcca
    59881 gaattgatcc taactggatc acaacttcaa aggttttcgc cgtgtcgccc tcaaacccca
    59941 acaagtgacc gacaataatc atatggcccg tgctggatac cggcagaaat tctgtcaatc
    60001 cttcgaccac acccaatatt gccgctatca gcagcgagtg catatcgctc atcaataaac
    60061 ccctaaatta ttaaaatgta ccgcttgtcc gaactactgc agtatgacca ggttataacc
    60121 gtttggttta acagctgtaa aattaattat tttctttcag attattgcca cgctcaatga
    60181 ttacgccaac attcgccgcc cgcgccactg cgcctggctt gctgagtttg atacgcaccc
    60241 acggcgagtt gaagcgtgct aacagcagct ccgccacctc ttccgccacg cgctccacca
    60301 gcgcaaaacg tgccccctcg acgtggctga ccaccgtttc tgcaatgtca gcgtagctga
    60361 ggcaatccgc cacatcatca cttttcgccg ctttacggtt atcccacgcc atttcgatat
    60421 cgaacactaa cttctgttcg atggtctgtt cccagtcgta aacaccaata gtggtgatta
    60481 ccgaaagttg ctctataaat acaatatcca tcacgtcctg cctgcttttt ggctaaccgg
    60541 ataccacttc cggcgaaatg tgcgtattat ccacagattc atcgttgaac acgaattttc
    60601 aaaacggaac agcttatgag tgcaatcgcg cctggaatga tcctcatcgc gtacctctgc
    60661 ggctccattt ccagtgccat tctggtttgc cgcttgtgtg ggctgcccga tccgcgaacc
    60721 agcggctccg gcaatccagg cgcaaccaat gtgttacgta tcggtggcaa gggagcagcc
    60781 gtagcagtac tgattttcga cgttctgaaa ggaatgttgc ccgtctgggg cgcgtatgaa
    60841 ttaggtgtca gccccttctg gctaggctta attgccatcg ccgcctgtct tggacacatc
    60901 tggcccgttt tcttcggatt taaaggagga aaaggcgttg ctaccgcttt tggtgccatc
    60961 gcacccattg gctgggatct caccggagta atggcgggaa cctggttact gaccgtgcta
    61021 ttgagcggat actcgtcgct gggagcgatt gtcagtgcac tgattgctcc gttttatgtc
    61081 tggtggttta agccacaatt caccttcccg gtttcgatgc tctcttgcct gatcctgctg
    61141 cgtcatcatg acaacatcca gcgtctgtgg cgtcgtcagg agacaaaaat ctggacgaaa
    61201 ttcaaaagaa agcgcgaaaa ggatcccgag tgatttctgg tggatctaca tgacctgata
    61261 gccttcatcg ggtttgccca gccgttgctg gcaccatgcc gccagaaatt ccacgcagac
    61321 acgtaatttc atgctgcgat agagcggctc ccggtaaaca gcccagatat tggcgctttg
    61381 tgcatactcc ggcaatactt gcaccagttt gccactctcc agaaacggca acacatccca
    61441 ctcggaacgc agcataatcc ctttgccctc cagcgcccat tgcagcacaa tctcgccgct
    61501 attggaggaa agatgcccgc taaccttcac cgattttttc tcctgaccgt tccccaactc
    61561 ccatattccg tgggtcatat cgcgctcttt ggtcaccaga cagtcatgac gacttaattc
    61621 ctgtagggat tgtggctgcg gatatttttg cagatactca ggtgctgcgc ataatattct
    61681 tttatttttt gtcaaaagat gcgcaatata ataatcagga atttcgtcat taatacgaat
    61741 atccagatca atattatcct gcactaaatc aatttgtcga tcgaacaatt caaaatgcac
    61801 ctgtaactca ggataattgc gcatcagttc ggtaatggct ggcgcaatat ggctgcgccc
    61861 aaaaccaaaa ctgcaaccaa tacgaatcat cccctccggg cgcgttttga tttgcgtgac
    61921 gtcatcgacc agtcgctgat attgggtgag gatttccagc gcatgttcgt aacaacgttg
    61981 accactttca gtcagcgcca cgccgcgagc ggagcggttc agcagcgttg ttgccaacgt
    62041 attttcaagg atttggatcc gcttggtgac aaatgcgggc gtctgcccaa gcgtcgccgc
    62101 tgcggcgcta aaactcccgc tgtggacgat ttctaccagt acctgcaaat ctttggctaa
    62161 aggccagcta ttcagcatcg ttaattatcc gcagttgtga taagcgcagt gtatttcgca
    62221 aaacattgcc attccctgct ctggcgcaat attcatactt attttcgcgt tgattcacga
    62281 actgttaatt acatattcac ggtggcaaaa aatataaaac cacattttta gtggtagttt
    62341 gtggcggtga atttttccag acaaatacag aaactggagt tgccatgatg agcgaaagta
    62401 ataagcaaca ggcagtgaat aagttgacgg agattgtcgc taactttacc gccatgattt
    62461 ctacccgaat gcctgatgac gtggtggata aactaaaaca gctaaaggat gccgaaacgt
    62521 cgtcgatggg gaaaattatc taccatacga tgttcgacaa catgcaaaaa gcgattgacc
    62581 tgaatcgtcc tgcctgtcag gacaccgggg agattatgtt cttcgttaaa gtcggttccc
    62641 gcttcccact gcttggcgag ctgcaaagca tactcaaaca agccgtggaa gaggcaaccg
    62701 tcaaagcgcc actacgtcac aatgcggtag aaatttttga cgaagtgaac acaggcaaaa
    62761 ataccggcag cggcgtaccg tgggtcacct gggatatcgt ccccgacggt gacgatgcgg
    62821 aaatcgaagt ttacatggca ggcggcggct gcacgctacc aggccgctcg aaagtgttaa
    62881 tgccgtcaga aggctacgaa ggcgtggtga aattcgtctt cgaaaatatc tccaccctcg
    62941 cagtaaacgc ctgtccaccg gtactggtgg gcgtgggcat cgccacctcg gtggaaaccg
    63001 ccgccgtgct ctcgcgaaaa gccattttgc gcccgattgg cagccgccac cccaatccaa
    63061 aagcggcaga gctggagcta cgcctggaag aaggactcaa ccgtctgggg attggtccac
    63121 aagggctgac tggcaacagt tcagtgatgg gcgtgcatat cgaatctgcc gctcgccatc
    63181 cgtcaaccat cggcgttgct gtttctacag gctgctgggc gcatcgtcgc ggcacactgc
    63241 tggttcatgc cgatctctcc tttgaaaatc tgtctcacac ccggagcgcg ttatgaaaaa
    63301 gatcctgaca accccgatca aagctgaaga tctgcaagat attcgcgtcg gcgatgtgat
    63361 ctacctgacc ggtacgctgg tgacctgccg cgacgtttgt caccgccgct tgatcgaact
    63421 gaaacgtccg atcccttacg atctcaacgg caaagcgatt ttccacgctg gccccatcgt
    63481 gcgcaaaaac ggcgacaaat gggagatggt ctccgtcggt ccgacaacca gtatgcgtat
    63541 ggaaagtttt gaacgtgaat ttatcgagca gaccggcgtg aaactggtgg tcggcaaagg
    63601 cggaatgggg ccgctgaccg aagaaggctg ccagaaattc aaggcgctac atgtgatttt
    63661 cccggcaggc tgcgcggtgc tggcggcaac ccaggtggaa gagattgaag aagtgcactg
    63721 gacagaactc gggatgccgg agtcactgtg ggtctgccgg gtcaaagagt tcggcccgct
    63781 gattgtctct attgataccc acggcaacaa cctgatagcc gaaaacaaaa agctgttcgc
    63841 cgaacgccgc gatcccatcg tggaagagat ctgcgagcac gtccattaca tcaaataacc
    63901 ctcccggaga ggctcgcccc ctctcctttt tcgcaggcat aacacgatga aaccttccac
    63961 tgaatggtgg cgataccttg cgccgctggc ggtcatcgcc attattgctc taattccggt
    64021 tcccgcaggg ctggagagtc atacctggct ctactttgcc gtttttactg gcgtaatcgt
    64081 tggactgatc ctcgaacccg tgccgggtgc cgtggtggcg atggtgggta tatccatcat
    64141 cgccatactc tctccctggc tgctgttcag ccccgaacag ctcgctcagc caggctttaa
    64201 attcactgca aaatccctct cgtgggccgt ttccggtttt tctaattcgg ttatctggct
    64261 gattttcgcc gcctttatgt ttggcacagg ctatgaaaaa accgggctgg ggcgacgtat
    64321 cgcgctgatt ctggtgaaaa agatggggca tcgcacgctg tttcttggct atgcggtgat
    64381 gttctccgag ctgatcctgg cacctgtcac accgtccaac tcggcgcgtg gtgcggggat
    64441 tatctatccc atcatccgta acctgccgcc gctctatcaa tcacaaccaa acgacagcag
    64501 ttcgcgcagt attggctcgt acatcatgtg gatggggatt gttgccgact gcgtgaccag
    64561 cgccattttc ctgacggcga tggctcctaa cttgctgtta attggactga tgaaaagcgc
    64621 atctcacgcc acgctgagtt ggggcgactg gttcctcggg atgttgccgc tcagtatttt
    64681 actggtcctg ctggttccct ggctggctta cgtgctgtac ccgccggtac tgaagtctgg
    64741 tgatcaggta ccgcgctggg cagagacgga actgcaggca atgggcccgc tctgttcgcg
    64801 tgaaaaacgg atgctggggc tgatggtagg cgcgctggtg ctgtggattt tcggcggtga
    64861 ttatatcgat gccgcgatgg tcggttacag cgtggtggcg ctgatgctgc ttctgcgcat
    64921 tatcagttgg gacgatattg tcagtaataa agcggcgtgg aacgttttct tctggctggc
    64981 ctcgcttatc accctcgcga ccggactcaa caacaccggt tttattagct ggtttggcaa
    65041 actgttagca ggcagcttaa gcggttattc gccaacgatg gtgatggtgg cgttgattgt
    65101 ggtgttttat ctactgcgct actttttcgc cagcgccacg gcgtatacct ccgctctcgc
    65161 accgatgatg atcgccgccg cgctggcgat gccggaaatc ccgctgccgg tgttctgcct
    65221 gatggttggc gcggcaattg gtctggggag cattcttaca ccatacgcca ccggacccag
    65281 tccgatttac tacggtagtg gttatctgcc aacggcggat tactggcgac tgggggcgat
    65341 ttttgggctg atattcctcg tgttgctgat gattaccggc ttactgtgga tgcccgtggt
    65401 gttgctttaa gtgaggacgg tgccggaaat tgctctccgg cacccaatag ttttacgcag
    65461 ccggtaactc cgccagcggc cagcgcggac gcacgctaac accgagatcc gccgtcgcgc
    65521 ctgctttaaa ccgcaccatt ccggcatagg cgatcatcgc accgttatca gtacaaaatt
    65581 ccggacgcgc gtagaacact tcgccgcggc gttttttcat catttccgcc agtttcgccc
    65641 gcagcgtgcg gttagcactc acgccgcccg ccatgaccag tcgcttaaag cccgtctgat
    65701 ccagtgctcg cttacactta atcatcaacg tatcaaccac cgcatcttca aaggcgcggg
    65761 cgatatcagc acgcgtctgg tcgtcggtgc cgttgtcacg aatcgtgttc gccgcaaagg
    65821 ttttcagacc agaaaagctg aaatccagcc ccggacggtc ggtcatcgga cgcgggaaaa
    65881 caaagcgccc ggcagtaccc tgagccgcca ttttcgacag taacggtccg ccaggataat
    65941 ccagccccag cagcttcgcg gttttatcaa acgcttcacc ggcggcatca tcgatagact
    66001 cgccgagcag ctcgtactga ccaatgccag tcacgctgat taactgcgta tgaccgccgg
    66061 aaaccagcag cgcaacaaac ggaaattccg gcgggttatc ttccagcatc ggcgctaaca
    66121 gatgcccttc catatggtgt acagggatcg ccggaacgtt ccaggcaaac gccagagaac
    66181 gccccacggt cgcgccaacc agcagtgcgc cgactaatcc agggcctgcg gtataggcca
    66241 cagcatcaat gtcttttgcc gttaaaccag actcctttag cgccgcctgg atcaacggta
    66301 cggttttacg cacatgatcg cgggaggcca gctcaggcac aacgcccccg tagtcagcgt
    66361 gcaatttcac ctgactatac aattggttgg ctaacaaacc tttttcatcg tcgtaaatgg
    66421 cgatgccggt ttcatcgcag gaagtttcaa tacccagtac acgcatgact tgttttacct
    66481 cgctttatta ccgcgcagtg taggaccaat gcgggttgat gtaaaacttt gttcgcccct
    66541 ggagaaagcc tcgtgtatac tcctcaccct tataaaagtc cctttcaaaa aaggccgcgg
    66601 tgctttacaa agcagcagca attgcagtaa aattccgcac cattttgaaa taagctggcg
    66661 ttgatgccag cggcaaaccg aattaatcaa aggtgagagg cacatgccgg taattaaagt
    66721 acgtgaaaac gagccgttcg acgtagctct gcgtcgcttc aagcgttcct gcgaaaaagc
    66781 aggtgttctg gcggaagttc gtcgtcgtga gttctatgaa aaaccgacta ccgaacgtaa
    66841 gcgcgctaaa gcttctgcag tgaaacgtca cgcgaagaaa ctggctcgcg aaaacgcacg
    66901 ccgcactcgt ctgtactaat tccccgagag cgttgctctc cgatcagacc gagttgtagt
    66961 tgtaaggccg tgcttccgaa aggaatgcgc ggcttatttt cgtttatgaa ttgctaaaaa
    67021 tcggggccta tggctggacg aatcccacgc gtattcatta atgatctgct ggcacgcacc
    67081 gacatcgtcg atctgatcga tgcccgtgtg aagctgaaaa agcagggcaa gaatttccac
    67141 gcgtgttgtc cattccacaa cgagaaaacc ccgtcattca ccgttaacgg tgagaaacag
    67201 ttttaccact gctttggatg tggcgcgcac ggcaacgcga tcgacttcct gatgaactac
    67261 gacaagctcg agttcgtcga aacggtcgaa gagctggcag caatgcacaa tcttgaagtg
    67321 ccatttgaag caggcagcgg ccccagccag atcgagcgcc atcagcggca aacgctttat
    67381 cagttgatgg acggtctgaa tacgttttac caacaatctt tacagcaacc tgttgccacg
    67441 tctgcgcgcc agtatctgga aaaacgcgga ttaagccacg aggttatcgc tcgctttgcg
    67501 attggttttg cgccccccgg ctgggacaac gtcctgaagc ggtttggcgg caatccagaa
    67561 aatcgccagt cattgattga tgcgggcatg ttggtcacta acgatcaggg acgcagttac
    67621 gatcgtttcc gcgagcgggt gatgttcccc attcgcgata aacgcggtcg ggtgattggt
    67681 tttggcgggc gtgtgctggg caacgatacc cccaaatacc tgaactcgcc ggaaacagac
    67741 attttccata aaggccgcca gctttacggt ctttatgaag cgcagcagga taacgctgaa
    67801 cccaatcgtc tgcttgtggt cgaaggctat atggacgtgg tggcgctggc gcaatacggc
    67861 attaattacg ccgttgcgtc gttaggtacg tcaaccaccg ccgatcacat acaactgttg
    67921 ttccgcgcga ccaacaatgt catttgctgt tatgacggcg accgtgcagg ccgtgatgcc
    67981 gcatggcgag cgctggaaac ggcgctgcct tacatgacag acggccgtca gctacgcttt
    68041 atgtttttgc ctgatggcga agaccctgac acgctggtac gaaaagaagg taaagaagcg
    68101 tttgaagcgc ggatggagca ggcgatgcca ctctccgcat ttctgtttaa cagcctgatg
    68161 ccgcaagttg atctgagtac ccctgacggg cgcgcacgtt tgagtacgct ggcactgcca
    68221 ttgatatcgc aagtgccggg cgaaacgctg cgaatatatc ttcgtcagga attaggcaac
    68281 aaattaggca tacttgatga cagccagctt gaacgattaa tgccaaaagc ggcagagagc
    68341 ggcgtttctc gccctgttcc gcagctaaaa cgcacgacca tgcgtatact tatagggttg
    68401 ctggtgcaaa atccagaatt agcgacgttg gtcccgccgc ttgagaatct ggatgaaaat
    68461 aagctccctg gacttggctt attcagagaa ctggtcaaca cttgtctctc ccagccaggt
    68521 ctgaccaccg ggcaactttt agagcactat cgtggtacaa ataatgctgc cacccttgaa
    68581 aaactgtcga tgtgggacga tatagcagat aagaatattg ctgagcaaac cttcaccgac
    68641 tcactcaacc atatgtttga ttcgctgctt gaactgcgcc aggaagagtt aatcgctcgt
    68701 gagcgcacgc atggtttaag caacgaagaa cgcctggagc tctggacatt aaaccaggag
    68761 ctggcgaaaa agtgatttaa cggcttaagt gccgaagagc gatcgggaag cccccgacag
    68821 ccgcactgag aggcagcggc aaatatataa gtacgccctc gtaattatcg ttggcggtaa
    68881 ataaccgttg gatttcagcg ttaacggctg aaggacatcg ggtcaatcgc ccaacaccaa
    68941 cctcatgaaa taagtgtgga taccgtctta tggagcaaaa cccgcagtca cagctgaaac
    69001 ttcttgtcac ccgtggtaag gagcaaggct atctgaccta tgccgaggtc aatgaccatc
    69061 tgccggaaga tatcgtcgat tcagatcaga tcgaagacat catccaaatg atcaacgaca
    69121 tgggcattca ggtgatggaa gaagcaccgg atgccgatga tctgatgctg gctgaaaaca
    69181 ccgcggacga agatgctgcc gaagccgccg cgcaggtgct ttccagcgtg gaatctgaaa
    69241 tcgggcgcac gactgacccg gtacgcatgt acatgcgtga aatgggcacc gttgaactgt
    69301 tgacccgcga aggcgaaatt gacatcgcta agcgtattga agacgggatc aaccaggttc
    69361 aatgctccgt tgctgaatat ccggaagcga tcacctatct gctggaacag tacgatcgtg
    69421 ttgaagcaga agaagcgcgt ctgtccgatc tgatcaccgg ctttgttgac ccgaacgcag
    69481 aagaagatct ggcacctacc gccactcacg tcggttctga gctttcccag gaagatctgg
    69541 acgatgacga agatgaagac gaagaagatg gcgatgacga cagcgccgat gatgacaaca
    69601 gcatcgaccc ggaactggct cgcgaaaaat ttgcggaact gcgcgctcag tacgttgtaa
    69661 cgcgtgacac catcaaagcg aaaggtcgca gtcacgctgc tgctcaggaa gagatcctga
    69721 aactgtctga agtattcaaa cagttccgcc tggtgccgaa gcagtttgac tacctggtca
    69781 acagcatgcg cgtcatgatg gaccgcgttc gtacgcaaga acgtctgatc atgaagctct
    69841 gcgttgagca gtgcaaaatg ccgaagaaaa acttcattac cctgtttacc ggcaacgaaa
    69901 ccagcgatac ctggttcaac gcggcaattg cgatgaacaa gccgtggtcg gaaaaactgc
    69961 acgatgtctc tgaagaagtg catcgcgccc tgcagaaact gcagcagatt gaagaagaaa
    70021 ccggcctgac catcgagcag gttaaagata tcaaccgtcg tatgtccatc ggtgaagcga
    70081 aagcccgccg tgcgaagaaa gagatggttg aagcgaactt acgtctggtt atttctatcg
    70141 ccaagaaata caccaaccgt ggcttgcagt tccttgacct gattcaggaa ggcaacatcg
    70201 gtctgatgaa agcggttgat aaattcgaat accgccgtgg ttacaagttc tccacctacg
    70261 caacctggtg gatccgtcag gcgatcaccc gttctatcgc ggatcaggcg cgcaccatcc
    70321 gtattccggt gcatatgatt gagactatca acaaactcaa ccgtatttct cgccagatgc
    70381 tgcaagagat gggccgtgaa ccaacgccgg aagaactggc tgaacgtatg ctgatgccgg
    70441 aagacaagat ccgcaaagtg ctgaagatcg ccaaagagcc aatctccatg gaaacgccga
    70501 tcggtgatga tgaagattcg catctcgggg atttcattga ggataccacc ctcgagctgc
    70561 cgctggattc tgcgaccacc gaaagcctgc gtgcggcaac acacgacgtg ctggctggcc
    70621 tgaccgcgcg tgaagcgaaa gttctgcgta tgcgtttcgg tatcgatatg aacaccgatc
    70681 acacgctgga agaagtgggt aaacagttcg acgttacccg cgaacgtatc cgtcagatcg
    70741 aagcgaaggc gctgcgcaaa ctgcgtcacc cgagccgttc tgaagtgctg cgtagcttcc
    70801 tggacgatta atcggtaggc cggatcaggc gttacgccgc acccggcacc tgtccctttg
    70861 cacaaacgcc accttttcgg tggcgttttt tatcgcccac gcaccaccag cgcctggtcc
    70921 agctcgcgat acgcttcaac cagtttctcc agtgaaacgc gacttaaacc actgggattt
    70981 ggcagcaccc aaatctgcgt cgaaccaatg ctgagcgttt gtttccccca ctgtgcaccg
    71041 cgctggctga atccctgttc atatgcttgt ttacccagaa tcgccaacgc ttgcggctga
    71101 taatcttcaa ttttttcaat cagcttacgc ccacctgcgt gtagctcttg ctttgaaatt
    71161 tcattggctt gcaccgttgg acggtctacc agtttggtga cgccacaacg ataatccagc
    71221 agatgctgtg cctcctgcgg cttcaactga cggtcggtaa acccggcctg atatatcacc
    71281 ttccagaagc gatttgccgg atgagcaaag ggaaaaccag tcccggcgga tgaaagccca
    71341 gggttgatac cgcaaaacac gacccgtaac cctggagcca aaatatcctc aaccatattt
    71401 actcctttct aacatcatca ggaaaagtat aacggattga aaatacattg tttataaaaa
    71461 cagcaggcgc gcggtaatgg ctggattgcg acacggagtt actttataat ccgctaccat
    71521 ggccccttag ctcagtggtt agagcaggcg actcataatc gcttggtcgc tggttcaagt
    71581 ccagcagggg ccaccagata tagcaaaggc tgacgagaaa tcgtcagcct ttttcttttt
    71641 atatatcagt tactttgcgt gccagtaagc cgctgcacgt acccgctgtg ggtcatactg
    71701 ttccgcttca aagcggcggc ttaaattctt aacgacttta ccttcgccgg ttatccagat
    71761 gaagtaatca tcggcaggga tttgcatctg cgccagacgc gcatctaccg cctgctcatc
    71821 atgtgccagc cattcgatat taaaaccatc aaggtgcgcg agataatcct gacaggcgtt
    71881 atcccgcacg ctaaccagcg cactaacttg cggtttaacg gcaagtttgc tcaacgtttc
    71941 caggcggcgg cgcaatgcgg gcattccgga ttcatcgcag acatacagct gatacgcgta
    72001 atcttccggc accaccagcg aaccgcgcgg acctgccacc gtgagtttat cgcccggttg
    72061 ggcctgcatt gcccagctac tggcgacgcc gtcttcgtga ataaagaaat caatcgccag
    72121 ttcatggcgt agttcgtcat acagtggcgt atagtcacgc gacggtgggc gtggtccttc
    72181 cggccagacg atgccctctt ccgttaccgt tggcggcaca aagtgagcgt caggttgagg
    72241 aaagaagagt ttgctgtgat cgtcaaagcc gcgcgatgta aaaccgtcca gcgcctcgcc
    72301 gccaaggaca atgcgctgaa aaccggcgct gatacgctca acgcgtagca cagtcagttc
    72361 acggaaccgc agatcattgc gaacgcgctg cgggtagcgg ggggagttat tcattgttat
    72421 cgccttcgtg atggtaatca gatatatcta aataattttc gcaaatgata atgattgtta
    72481 atcatgataa atgcaagcga tttgtagaac tgatatatct atagtctgat aagacgaacc
    72541 gcctcttctc aggcatcatt actcaacgcc ggatgcggcg tgaacgcctt atccggccta
    72601 cgtgtgagat gagtccacat ttaaaaatca tttttattct tgcaagagtg ctcatatcag
    72661 atataaatta gatatatcta attaagcaaa aggaggctga tatgagccat catcacgaag
    72721 ggtgttgtaa acatgaaggc cagccacgcc atgagggctg ctgcaaaggt gagaagtcag
    72781 aacacgagca ctgcggacac ggtcaccagc atgaacacgg tcaatgttgc ggtggtcgcc
    72841 acggtcgcgg cggcggtcgt cggcaacgtt tctttggtca cggtgaatta cgtctggtga
    72901 ttctggatat tctctcgcgc gatgacagcc acggttacga attgattaaa gcgattgaga
    72961 atctaaccca ggggaattac accccaagcc cgggcgtcat ctacccaacg ctggattttc
    73021 tgcaggagca gtcgctgatt accatccgcg aagaggaagg tggtaagaag cagattgcac
    73081 tgaccgaaca aggcgcgcag tggctggaag aaaaccgcga acaggtggag atgattgaag
    73141 aacgcatcaa agcgcgttgc gttggtgctg cgctgcgcca gaacccgcaa atgaagcggg
    73201 cgctggataa ttttaaagcg gtgctggatt tacgcgtcaa ccagagtgat atcactgatg
    73261 cacaaataaa aaagatcatt gcggtgatcg accgcgccgc ttttgatatt acgcaactgg
    73321 attaatcacc gattgccgga tgcggcgtac cctacatacc ccgtaggcct gataagatgc
    73381 gccagcatcg catcaggcat tgtgctccaa ccgccgaatc ctgcataccg attaatgcag
    73441 caccgtcacc gcgtcttcca gtcggctggc gcggtgtttc accatctccg acacctgcgc
    73501 actctcttcc accagctcgg catttttctg ggtgatgagg ttaagctcat ccactgcacg
    73561 ggtcaggctg gaaagaccat cggcctgttc cagcgttgaa tggctaatct gggcaatcaa
    73621 ctgggtgacg tttttcacct gtgccacaat atcttccatc gtacgtccgg cggcgtgtac
    73681 ctgctgcgaa ccggattgca ccttatcagc actggcatca atcagcttgc gaatatcgtt
    73741 ggcagcattg gcgctgcggc tggctaaatg acgcacttcc cccgccacca ctgcaaaacc
    73801 tttgccctgt tcgccggctc gcgccgcttc caccgccgca ttcagggcca gaatattggt
    73861 ctgaaacgca atatcgttaa tcagcgaagt aatggtgcca atgcgctggg tactgtcggc
    73921 gatatcgtcc atagtcttga tcaccgtggt catcgcctcc ccgccctgca ctgccgcatt
    73981 actggcagtg attgacagtt tatcggcagc cgacgccgtg gcagagttct gtttcaccga
    74041 cgccgccatt tggttcatgg tcgccaccgt ttgctgaacg ttatcaactg tctgctgggt
    74101 atgttcgttc agttcatcgg tgcctttcgc cagcgtctca ctgccatttc tgacactgga
    74161 cacctggctt gagacatcgt taattagcca acggcacatc aggccaagct gccctaccgc
    74221 gcgtaatgtc agccccagct catcgctgcg attcagatgc tcaacactat tacgctctcc
    74281 ggtcgccacc ttcagtgcct gacgggcgac attttctatc gggcggacaa tctgccattc
    74341 aaaacaggcg cttgccaaca ataccactaa cgcacagagg atatacgtca ccaccggggc
    74401 agcaacaaac caaagcatgg ccgccagcaa gataaacatc agggtcatca ctccacgcgc
    74461 ccgccagcga agcggtaatg aaggcagttt acccagccag cctttacgca ccaccaggcc
    74521 tttatgaata cgcttaccgg tacgtccggc gttcagcgct ttgtacagcg gctccaccgc
    74581 cgcgatctct tcatccgtcg cccgggtacg aatcgacata tagccactga tttttccctc
    74641 gcgcaccatc ggtaccgcat tggcccgcac ccaataatgg tcaccatttt tgcggcgatt
    74701 tttcacgatg ccgctccagg gctccccttt tttcagggtg aaccacatat ctgcaaacgc
    74761 cgcttttggc atatccgggt gacgcaccat gttgtgcggc tgcccttgta actcttgcaa
    74821 ggtatagccg ctcacctgca caaaagtgtc attagcatga gtgatatagc tttgcagatc
    74881 ggtagtggac atcagagtgg tatcgtccgc cagcggggta ttttgctggg tgacatacgg
    74941 atgagaagac atgttcgcgt cctgtgcagg ttatatggtt gttaacttct tgtcagagtt
    75001 tatgtcggcc ccgctgcggt tatctttaac cgattaattt gatttagatc gcaatttgcg
    75061 attaaagcac aaatctaatt cgttgattta aaatactttc actctgttac tatacgaaaa
    75121 cgttaattat cttgcccaaa aatcaggcaa ttattgccct gaaaacgtgc atttgcgcag
    75181 caattatcaa atccataccc gacaaaaacc gtgcaaaata acaacggatg ttaacaaata
    75241 gcattaaata ttgcgcaaat gataaccaca tttgtgttta tcccgatttt cgcgatcgca
    75301 gccggagtgg cgcaatccct gcaatactta aatcggtatc atgtgatacg cgagcctccg
    75361 gagcatattt tgaacaggtt accttcgagc gcatcggctt tagcgtgcag cgcccacgcc
    75421 ctgaatctca ttgagaagcg aacgctggat catgaggaga tgaaagcact taaccgagag
    75481 gtgattgaat acttcaaaga gcatgtcaat ccggggtttt tagagtatcg caaatctgtt
    75541 accgccggcg gggattacgg agccgtagag tggcaagcgg gaggtttaaa tacgcttgtc
    75601 gacacccagg gacaggagtt tatcgactgc ctgggaggtt ttggaatttt caacgtgggg
    75661 caccgtaatc cagttgtggt ttccgccgta cagaatcaac ttgcgaaaca accactgcac
    75721 agccaggagc tactcgatcc gttacgggct atgctggcga aaacccttgc tgcgctaacg
    75781 cccggtaaac tgaaatacag cttcttctgt aatagcggca ccgagtccgt tgaagcggcg
    75841 ctgaagctgg cgaaggctta tcagtcgccg cgcggcaaat ttacttttat tgccaccagc
    75901 ggcgcgttcc acggtaaatc acttggcgcg ctgtcggcca cggcgaaatc gaccttccgc
    75961 aaaccgttta tgccactgct gccagggttc cgtcatgtgc cgtttggcaa tatcgaagcc
    76021 atgcgcacgg ctcttaatga gtgcaaaaaa accggtgatg atgtggctgc ggtgatcctc
    76081 gaaccgattc agggcgaagg tggcgtaatt ctgccgccgc cgggctatct caccgccgtg
    76141 cgtaagctgt gcgatgagtt cggcgcactg atgatcctcg atgaagtaca aacgggcatg
    76201 gggcgcacgg gcaagatgtt tgcctgcgaa catgagaacg tccagccaga cattctgtgt
    76261 ctggcaaaag ccctcggcgg cggcgtgatg ccgattggcg caaccatcgc tactgaagag
    76321 gtgttctcag tcttgttcga caacccgttc ctgcatacca ccacctttgg cggcaacccg
    76381 ctggcctgtg cggcggcgct ggcgaccatc aatgtgttgc tggaacaaaa cttaccggct
    76441 caggctgaac aaaaaggcga tatgttgctg gacggtttcc gtcaactggc gcgggaatat
    76501 cccgatctgg tgcaggaagc acgtggtaaa gggatgttga tggcgattga gtttgttgat
    76561 aacgaaatcg gctataactt tgccagcgag atgttccgcc agcgcgtact ggtggccgga
    76621 acgctcaata acgccaaaac gatccgcatt gaaccgccac tgacactgac cattgaacag
    76681 tgtgaactgg tgatcaaagc ggcgcgtaag gcgctggcgg ccatgcgagt aagtgtcgaa
    76741 gaagcgtaat atcaaatcgg atggcgatac gacgtcgcca tccgatcttt ttatacgata
    76801 cgtacgcccg caggcatcat ccgctccggc gttaacagca cgctttcact gccgtcatcg
    76861 gtttccgcac acaacagcat gcattccgac gtttcaccgc gcattttcgc tttttgcaga
    76921 ttgcataaga ccaccaccgt ttttcccatc aactcttctt cgctgtagta cggcaccagg
    76981 ctggtcacgg tttgcagtgt tttttccccc acatcaacct gtacgatgta cagcttgtcg
    77041 gcgttttcat ggcgtttcac ttccacaatc tttccgacgc gcatttccag acgtgcaaaa
    77101 tcagcgtaag ccacggtttc cattgcttcc tcccttcgag taaaatttta ctaaactata
    77161 gcaaaagttt ttctcaatcc tgtaggctaa aaatggagaa tgcaggcgtg atcacattcg
    77221 tgagccgctg tgttaccgtt acagcgtcaa agaaacgcgc tttatttact gaaaacaggt
    77281 gacccgataa gcacttcctc tacaatgggg gcgcacatca gggaaagtaa aaaaggtaaa
    77341 catggcaaca ctaaaagaca tcgcaatcga agctggcgta tccctggcga cagtatccag
    77401 ggtcttaaat gacgatccga cattgaatgt gaaagaagag acgaaacatc gcattctcga
    77461 gatcgccgaa aagctggagt acaagaccag tagcgcccgt aaactacaga caggcgcagt
    77521 caaccaacac catattctgg ctatctacag ctaccagcag gagctggaga tcaacgatcc
    77581 ttactatctg gcgatccgcc acggcattga aactcagtgc gaaaagctgg cgatcgaact
    77641 gaccaactgt tatgaacaca gcggattacc agacattaaa aacgtcaccg gtattttaat
    77701 tgtcggcaaa cccacacccg ccctgcgcgc cgccgcctgt gcgttgaccg acaatatctg
    77761 ttttatcgac tttcacgaac ccggcagcgg ttacgatgcg gtggatatcg atctggcacg
    77821 catcagtaaa gaaatcatcg acttctatat caaccagggc gttaatcgca ttggttttat
    77881 tggcggtgaa gatgagcctg gcaaggcgga tattcgtgag gtcgcctttg cggaatatgg
    77941 ccgtctgaaa caagtagtac gcgaagagga tatctggcgc ggcggttttt ccagttcgtc
    78001 gggttatgaa ctggcaaaac agatgctggc gcgggaagac tatccgaagg cactgtttgt
    78061 tgcttccgat tccattgcta tcggcgtact gcgggcaatt catgaacgtg gcctgaacat
    78121 cccacaggat atttcgctta tcagcgttaa cgatatcccc accgcgcgat ttacctttcc
    78181 gccgctctcc accgtgcgca tccattccga gatgatggga agtcagggcg ttaacctggt
    78241 gtatgaaaaa gcccgcgatg gtcgcgcgct gccgctgtta gtcttcgttc ccagcaaatt
    78301 aaaactgcgc ggcacgaccc gttaaatccc cttacacact gtccggcaat catttttgcc
    78361 ggacagtgct gccgtttatt ttcgtgatcc agttaaagta aatgcattta cctgctattt
    78421 tttagtaaaa attttactaa actccccagc aattacacaa actaccatca ccatgaatgg
    78481 ttccgatttc tctctaccgg gaggccctat gaatcgctgg gaaaacattc agctcaccca
    78541 cgaaaaccga cttgcgccgc gtgcgtactt tttttcatat gattctgttg cgcaggcgcg
    78601 tacctttgcc cgcgaaacga gcagcctgtt tctgccctta agcggtcagt ggaatttcca
    78661 cttttttgac catccgctgc aagtgccaga agccttcacc tctgagttaa tggctgactg
    78721 ggggcatatt accgtccccg ccatgtggca aatggaaggt cacggcaaac tgcaatatac
    78781 cgacgaaggt tttccgttcc ccatcgatgt gccgtttgtt cccagcgata acccaaccgg
    78841 tgcctatcaa cgtattttca ccctcagtga cggctggcag ggtaaacaga cgctgattaa
    78901 atttgacggc gtcgaaacct attttgaagt ctacgttaac ggtcagtatg tgggtttcag
    78961 caagggcagt cgcctgaccg cagagtttga catcagcgcg atggttaaaa ccggcgacaa
    79021 cctgttgtgt gtgcgcgtga tgcagtgggc ggactctacc tacgtggaag accaggatat
    79081 gtggtggtcg gcggggatct tccgcgatgt ttatctggtc ggaaaacaac taacacatat
    79141 taacgatttc accgtgcgta ccgactttga cgaagcctat tgcgatgcca cgctttcctg
    79201 cgaagtggtg ctagaaaatc tcgccgcctc ccctgtcgtc acgacgctgg aatataccct
    79261 gtttgatggc gaacgcgtgg tgcacagcag cgccattgat catttggcaa ttgaaaaact
    79321 gaccagcgcc agctttgctt ttactgtcga acagccacag caatggtcag cagaatcccc
    79381 ttatctttac catctggtca tgacgctgaa agacgccaac ggcaacgttc tggaagtggt
    79441 accacaacgc gttggcttcc gtgatatcaa agtgcgcgac ggtctgttct ggatcaataa
    79501 ccgttatgtg atgctgcacg gcgtcaaccg tcacgacaac gatcatcgca aaggccgcgc
    79561 cgttggaatg gatcgcgtcg agaaagatct ccagttgatg aagcagcaca acatcaactc
    79621 cgtgcgtacc gctcactacc cgaacgatcc gcgtttttac gaactgtgtg atatctacgg
    79681 cttgtttgtg atggcggaaa ccgacgtcga atcgcacggc tttgctaatg tcggcgatat
    79741 cagccgtatt accgacgatc cgcagtggga aaaagtctac gtcgagcgca ttgttcgcca
    79801 tattcacgcg cagaaaaacc atccgtcgat catcatctgg tcgctgggca atgaatccgg
    79861 ctatggctgt aacatccgcg cgatgtacca cgcagcgaaa gcgctggatg acacgcgact
    79921 ggtgcattac gaagaagatc gcgatgctga agtggtcgat attatttcca ccatgtacac
    79981 ccgcgtgccg ctgatgaatg agtttggtga atacccgcat ccgaagccgc gcatcatctg
    80041 tgaatatgct catgcgatgg ggaacgggcc gggcgggctg acggagtacc agaacgtctt
    80101 ctataagcac gattgcattc agggacatta tgtctgggaa tggtgcgacc acgggatcca
    80161 ggcgcaggat gacaacggca atgtctggta taaattcggc ggcgactacg gcgactatcc
    80221 caacaactat aacttctgtc ttgatggttt gatctattcc gatcagacgc cgggaccagg
    80281 cctgaaagag tacaaacagg ttattgcgcc ggtaaaaatc cacgcgctgg atctgactcg
    80341 cggcgagttg aaagtcgaaa ataaactgtg gtttaccacg cttgatgact acaccctgca
    80401 cgtagaagtg cgcgccgaag gtgaaacgct cacgacgcgg cagattaaac tgcgcgacgt
    80461 tgcgccgaac agcgaagccc ccttgcagat cacgctgccg cagctggacg cccgcgaagc
    80521 gttcctcaac attacggtga ccaaagattc ccgcacccgc tacagcgaag ccgggcattc
    80581 tatcgccact tatcagttcc cgctgaagga aaacaccgcg cagccagtgc ctttcgcacc
    80641 aaataatgcg cgtccgctga cgctggaaga cgatcgtttg agctgcaccg ttcgcggcta
    80701 caacttcgcg atcaccttct caaaaatgag tggcaaaccg acatcctggc aggtgaatgg
    80761 cgagtcgctg ctgacccgcg agccaaagat caacttcttc aagccaatga tcgacaacca
    80821 caagcaggag tacgaagggc tgtggcaacc gaatcatttg cagatcatgc aggaacatct
    80881 gcgcgacttt gccgtagaac agagcgatgg tgaagtgttg atcatcagcc gcacggttat
    80941 agcaccgccg gtgtttgact tcgggatgcg ctgcacctac atctggcgca tcgctgccga
    81001 tggccaggtt aacgtggcgc tttccggcaa gcgttacggc gactatccgc acatcattcc
    81061 gtgcatcggt ttcaccatgg ggattaacgg cgaatacgat caggtggcgt attacggtcg
    81121 tggaccgggc gaaaactacg ccgacagcca gcaggctaac atcatcgata tctggcgcag
    81181 caccgtcgat gccatgttcg agaactatcc cttcccgcag aacaacggta accgtcagca
    81241 tgtccgctgg acggcactga ctaaccgcca cggcaacggt ctgctggtgg ttccgcagcg
    81301 cccaattaac ttcagcgcct ggcactatac ccaggaaaac atccacgctg cccagcactg
    81361 taacgagctg cagcgcagtg atgatatcac cctgaatctc gaccaccagc tgcttggcct
    81421 cggttccaat tcctggggca gcgaggtgct ggactcctgg cgcgtctggt tccgtgactt
    81481 cagctacggc tttacgttgc tgccggtttc tggcggagaa gctaccgcgc aaagcctggc
    81541 gtcgtatgag ttcggcgcag ggttcttttc cacgaatttg cacagcgaga ataagcaatg
    81601 aggatcatcg ataacttaga acagttccgc cagatttacg cctctggtaa gaagtggcaa
    81661 cgctgcgttg aagcgattga aaatatcgac aacattcagc ctggcgtcgc ccactccatc
    81721 ggtgactcat tgacttaccg cgtggagaca gactccgcga ccgatgcgct atttaccggg
    81781 catcgacgct attttgaagt gcattactac ctgcaagggc agcaaaaaat tgaatatgcg
    81841 ccgaaagaga cattacaggt agtggaatat tatcgtgacg aaactgaccg tgaatattta
    81901 aaaggctgcg gagaaaccgt tgaggtccac gaaggacaaa tcgttatttg cgatatccat
    81961 gaagcatatc ggtttatctg caataacgcg gtcaaaaaag tggttctcaa agtcaccatc
    82021 gaagatggtt attttcataa caaataacaa ctacggcggc aaaaggagtt tgccgccacc
    82081 gctaccctac tcattttcgg agatgtgtta tgtctgatac caaacgtaat acaatcggca
    82141 aattcggctt gctctcgctg acttttgccg ccgtttacag ctttaacaac gttatcaata
    82201 ataatattga gcttggactg gcctcggcac cgatgttttt cctcgcgacg attttttatt
    82261 ttattccctt ctgtctgatc atcgcagaat ttgtttcgtt aaataaaaac tcagaagccg
    82321 gtgtctacgc gtgggtaaaa agttcgctgg gcggacgttg ggcatttatt actgcctata
    82381 cctactggtt cgtaaacctg ttctttttca cctcgctgtt gccgcgcgtt attgcttatg
    82441 cttcgtatgc cttcctcggc tacgaatata ttatgacgcc ggttgccacc accattatca
    82501 gtatggtgct gttcgccttc tctacctggg tttccaccaa cggggcgaaa atgctggggc
    82561 caattacctc cgtcacttca acgctgatgc tgctgttaac gctctcctac attttactgg
    82621 caggtacggc gctggttggc ggcgtacagc ctgctgaccc catcaccgtt gacgcgatga
    82681 tcccgaactt caactgggcg ttcctcggcg ttaccacctg gatttttatg gccgcaggtg
    82741 gcgcggagtc cgtcgctgtg tacgttaacg acgtcaaagg cggttcgaaa tcgttcgtta
    82801 aagtgatcat cctcgccggg atttttatcg gcgtactata ttccgtctcc tcggtgctga
    82861 ttaacgtctt cgtcagcagc aaagagttga aatttactgg cggatcggtg caggtattcc
    82921 acggcatggc ggcgtatttt ggtctgccgg aagcgttgat gaatcgcttt gtcggtctgg
    82981 tgtcctttac cgcaatgttc ggttccctgc tgatgtggac cgcaacgccg gtgaaaattt
    83041 tcttctccga aatcccggaa ggcatctttg gtaagaaaac cgtagaactg aacgaaaacg
    83101 gcgttccggc gcgcgcagcg tggatccagt tcctgatcgt catcccgctg atgattatcc
    83161 cgatgctcgg ttccaatacc gtgcaggatc tgatgaatac cattattaat atgaccgccg
    83221 cagcgtccat gcttccgccg ttattcatca tgctggctta cctgaattta cgcgccaaat
    83281 tagatcacct gccacgcgat ttccgtatgg gctcccgccg caccggtatt atcgttgttt
    83341 caatgctgat tgcgatattt gccgtagggt ttgtcgcttc gacattcccg actggcgcga
    83401 atattctgac catcattttt tataacgtcg gcggtattgt tatcttcctt ggctttgcgt
    83461 ggtggaaata cagtaaatat ataaagggat taacggctga agagcgccat attgaagcga
    83521 cgccagccag caatgttgat taaacagact aataaaaaat gagcaggcat tttccctctt
    83581 cctggatgag gagagggaaa atagtttctg cctttatatt tttataatta caacgataaa
    83641 aggctgtact ttttctttag ctcatggatt aacacaatga aattaatcac tgcaccatgc
    83701 agagcattac ttgctctgcc gttttgctac gccttttctg cggcaggaga agaagcacgt
    83761 ccggcagaac atgacgacac aaaaacaccc gcaattacct cgacatcttc tccttcattt
    83821 cgtttttacg gcgaattagg ggttggtgga tatatggatt tagagggtga gaataaacat
    83881 aaatacagcg acggcaccta tattgaaggt ggcctggaga tgaagtacgg ctcctggttc
    83941 ggcctgattt acggcgaagg ctggaccgtg caggccgacc acgacggcaa tgcctgggtg
    84001 cctgaccata gctggggcgg tttcgaaggt gggattaacc gtttctatgg cggttatcga
    84061 accaatgatg gcaccgaaat catgctcagt ctgcgtcagg attcctcgct ggatgacctg
    84121 caatggtggg gcgacttcac acccgatctg ggctacgtca ttcccaatac ccgcgacatt
    84181 atgaccgcgc tgaaggtgca gaacttaagc ggcaactttc gttatagcgt caccgccaca
    84241 cctgccggac atcatgacga aagcaaagca tggctacatt ttggcaagta cgatcgctac
    84301 gacgacaaat acacctatcc ggcaatgatg aacggttaca tccagtatga ccttgccgaa
    84361 ggcatcacct ggatgaatgg tctggaaatt accgacggca caggacaact ctatctcacg
    84421 ggcctgctta cccctaactt tgccgctcgc gcctggcacc ataccggacg tgccgacggg
    84481 ttggacgtac cgggaagtga aagcgggatg atggtgagcg ccatgtatga agcgttaaag
    84541 ggcgtttatc tctccaccgc ttacacctac gccaaacatc gccctgacca cgctgacgat
    84601 gaaaccacct ctttcatgca gtttggtatc tggtacgaat atggcggcgg acgtttcgca
    84661 acggcttttg atagccgctt ctacatgaaa aatgcctcta acgatcccag cgaccaaatc
    84721 ttcctgatgc aatatttcta ctggtaataa ggactgtaat ttcatgaaaa tcaaaactat
    84781 tttaacgcca gtaacctgcg ctctgctgat aagtttttcc gcccatgccg ctaacgccga
    84841 caattataaa aacgtgatta accgtactgg tgcgccgcag tacatgaagg attacgatta
    84901 cgacgatcac cagcgtttta atccgttttt cgatctcgga gcctggcatg gtcatctgtt
    84961 gccagacggc cctaacacca tgggcggttt tccaggcgtt gcgctgctga cggaagagta
    85021 catcaacttt atggccagca atttcgaccg cctgaccgtc tggcaggacg gcaagaaagt
    85081 cgacttcacg ctggaggcat acagtattcc cggcgcgctg gtgcaaaaac tgacagcaaa
    85141 agatgtgcag gtcgaaatga ctctgcgctt cgccacgccg cgcacgtcac tactggaaac
    85201 taaaatcatc agcaataaac cgctggatct ggtgtgggat ggcgaactgc tggaaaaact
    85261 ggaagcgaaa gaagggaaac cgctttccga taaaaccatt gctggcgaat accctgacta
    85321 ccagcgcaaa atcagcgcca cccgtgatgg cctgaaagtc acctttggca aagtgcgcgc
    85381 cacttgggat ctgctgacct ccggtgaatc agaatatcag gtgcataaat ccctgccggt
    85441 gcagactgaa atcaacggta atcgctttac cagtaaggcg catatcaacg ggtcgacgac
    85501 gctctatacc acctactccc acctgctgac cgctcaggaa gttagcaaag agcaaatgca
    85561 gatccgcgat attctggcgc gtccggcgtt ttacctcacc gcctcgcagc aacgctggga
    85621 agagtatctg aagaaagggt taaccaatcc ggatgcgacg ccggaacaga cgcgcgtcgc
    85681 agtaaaagca atcgaaacgc tcaacggtaa ctggcgttcg cctggcgggg cggtgaaata
    85741 taacaccgtc acgccgtcgg tgaccgggcg ctggttctcc ggcaatcaga cctggccgtg
    85801 ggatacctgg aagcaggcgt ttgcgatggc gcatttcaat ccggacatcg ccaaagagaa
    85861 tatccgcgcg gtcttctcct ggcagatcca gcctggcgac agcgtgcgtc cgcaggatgt
    85921 gggctttgtc cccgacctga tagcgtggaa ccttagcccc gagcgtggtg gcgatggcgg
    85981 caactggaac gaacgtaata ccaaacccag ccttgccgcc tggtcggtga tggaagtgta
    86041 taacgtcacc caggataaag cctggttggc agagatgtac ccgaaactgg tggcctatca
    86101 tgactggtgg ttacgtaacc gcgatcataa cggcaacggc gtgccggaat atggcgcgac
    86161 ccgcgacaaa gcccacaaca ctgagagcgg cgagatgctg tttacggtga agaaaagcga
    86221 caaagaagag acgcagtctg ggctgaacaa ctacgcccgc gtggtggaga aaggccagta
    86281 cgacagtctg gaaattccgg cacaggttgc tgcatcgtgg gaatcggggc gtgatgatgc
    86341 cgccgtcttt gggtttatcg acaaagaaca gctggataaa tatgtcgcaa gcggcggcaa
    86401 acgtagcgac tggacggtga aattcgccga aaaccgcagt caggacggaa cgttgctggg
    86461 gtactcgcta ttgcaggagt cggtggatca ggccagctat atgtacagcg ataaccatta
    86521 tctggcggag atggcgacga ttctcggtaa accggaagag gctaagcgct atcgccagtt
    86581 ggcacagcag ctcgcggact acatcaacac ctgtatgttc gacccggcta cgcagtactt
    86641 ctatgacgtg cgtattgaag ataaaccgct ggcgaacggc tgcgcgggca aaccgattgt
    86701 tgagcgcggt aaagggccgg aaggctggtc gccgctgttt aacggtgcgg caacgcaggc
    86761 caatgccgac gcggtggtga aggtgatgct cgatcctaaa gagttcaata cctttgttcc
    86821 gctgggaacg gcggcgttaa ccaatccggc ttttggcgct gatatctact ggcgcgggcg
    86881 cgtatgggtg gatcagttct ggtttggtct gaaagggatg gagcgttacg gttatcgtga
    86941 tgatgccctg aagctggcgg atacgttctt ccggcatgcc aaaggattaa ccgccgatgg
    87001 cccaattcag gagaattaca acccgctgac tggcgcgcag caaggcgcac caaatttctc
    87061 ctggagtgcc gcacatttgt atatgttgta taacgatttt ttccgtaagc aataatttgc
    87121 aacggctggc ggatgcggcg tgaacgcctt atccgcccta catattcgtt cccgtaggct
    87181 ggataagacg cgacaagcgt cgcatccggc atctgcaccg cgccaccggc ggatgctgcg
    87241 tgaacgcctt atccgcccta catgttcgtt cccgtaggct ggataagacg cgacaagcgt
    87301 cgcatccggc atctgcacct cgccactggc ggatgcggcg tgaacgcctt atccgcccta
    87361 catgttcgtt cccgtaggct ggataagacc cgacaagcgt cgcatccggc gtctgcaccg
    87421 cgccactggc ggatgcggcg tgaacgcctt atccgcccta catgttcgtt cccgtaggct
    87481 ggataagacg cgacaagcgt cgcatccggc atctgcaccg cgccggcgga tgcggcgtga
    87541 acgccttatc cgccctaca
//
LOCUS       DAZFKF010000007        85130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFKF010000007 DAZFKF010000000
VERSION     DAZFKF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 85130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.007"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(83..754)
                     /gene="queE"
                     /locus_tag="ACHKA1_000594"
     CDS             complement(83..754)
                     /gene="queE"
                     /locus_tag="ACHKA1_000594"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO5678297.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            927..1529
                     /locus_tag="ACHKA1_000595"
     CDS             927..1529
                     /locus_tag="ACHKA1_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFO5678298.1"
                     /translation="MLRIFIVIIFIFNLSGCGYNDIQTYDEQVNASWSEVLNQYQRRA
                     DLIPNLVASIKGYSSHERDVLEAVTLARSQANRASSDLQQTPGDEQKLQAWQQAQAQV
                     TRTLGQLTIISERYPELKSQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKF
                     PAVLTAKVMDYTPKKNYLPDDVAAVSKAPTIDFSQNANAH"
     gene            1543..2214
                     /locus_tag="ACHKA1_000596"
                     /pseudo
     CDS             1543..2214
                     /locus_tag="ACHKA1_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            2451..3599
                     /locus_tag="ACHKA1_000597"
     CDS             2451..3599
                     /locus_tag="ACHKA1_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO5678299.1"
                     /translation="MRIFLLLLMLCSFQVFAERLPSVNDLTGTLTTEEQSVLNQQLQT
                     LEQQNHLQVAVLVTPTTGGKNIKLAASQSYGLYHWKPIGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEEMLPPKQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWRFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSVVCFSLLLATTCKQRLAVVFNMCRVFPMMLAPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASIKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSGRW"
     gene            3596..4498
                     /locus_tag="ACHKA1_000598"
     CDS             3596..4498
                     /locus_tag="ACHKA1_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO5678300.1"
                     /translation="MIVMRLIFILLALWSLPGLAQQIAVPELRQQVTDITGTLRTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKERASRKGSSSSSSG
                     GGSSGGGFSGGGGSSGGGGASGRW"
     gene            complement(4558..5856)
                     /gene="eno"
                     /locus_tag="ACHKA1_000599"
     CDS             complement(4558..5856)
                     /gene="eno"
                     /locus_tag="ACHKA1_000599"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO5678301.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(5944..7581)
                     /gene="pyrG"
                     /locus_tag="ACHKA1_000600"
     CDS             complement(5944..7581)
                     /gene="pyrG"
                     /locus_tag="ACHKA1_000600"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO5678302.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(7809..8600)
                     /gene="mazG"
                     /locus_tag="ACHKA1_000601"
     CDS             complement(7809..8600)
                     /gene="mazG"
                     /locus_tag="ACHKA1_000601"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO5678303.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVIDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(8671..9006)
                     /gene="mazF"
                     /locus_tag="ACHKA1_000602"
     CDS             complement(8671..9006)
                     /gene="mazF"
                     /locus_tag="ACHKA1_000602"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO5678304.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(9006..9254)
                     /gene="mazE"
                     /locus_tag="ACHKA1_000603"
     CDS             complement(9006..9254)
                     /gene="mazE"
                     /locus_tag="ACHKA1_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO5678305.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNGITPENLHENIDWGEPKDKEVW"
     gene            complement(9332..11566)
                     /gene="relA"
                     /locus_tag="ACHKA1_000604"
     CDS             complement(9332..11566)
                     /gene="relA"
                     /locus_tag="ACHKA1_000604"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO5678306.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(11614..12915)
                     /gene="rlmD"
                     /locus_tag="ACHKA1_000605"
     CDS             complement(11614..12915)
                     /gene="rlmD"
                     /locus_tag="ACHKA1_000605"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO5678307.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYSRAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            12972..15728
                     /gene="barA"
                     /locus_tag="ACHKA1_000606"
     CDS             12972..15728
                     /gene="barA"
                     /locus_tag="ACHKA1_000606"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO5678308.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(15961..17301)
                     /gene="gudD"
                     /locus_tag="ACHKA1_000607"
     CDS             complement(15961..17301)
                     /gene="gudD"
                     /locus_tag="ACHKA1_000607"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO5678309.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(17322..18662)
                     /gene="gudX"
                     /locus_tag="ACHKA1_000608"
     CDS             complement(17322..18662)
                     /gene="gudX"
                     /locus_tag="ACHKA1_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO5678310.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVEAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQREAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTQNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(18664..20016)
                     /gene="gudP"
                     /locus_tag="ACHKA1_000609"
     CDS             complement(18664..20016)
                     /gene="gudP"
                     /locus_tag="ACHKA1_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO5678311.1"
                     /translation="MSSLSQAASRVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(20451..20900)
                     /gene="yqcA"
                     /locus_tag="ACHKA1_000610"
     CDS             complement(20451..20900)
                     /gene="yqcA"
                     /locus_tag="ACHKA1_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO5678312.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(20918..21700)
                     /gene="truC"
                     /locus_tag="ACHKA1_000611"
     CDS             complement(20918..21700)
                     /gene="truC"
                     /locus_tag="ACHKA1_000611"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO5678313.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(21700..22029)
                     /gene="yqcC"
                     /locus_tag="ACHKA1_000612"
     CDS             complement(21700..22029)
                     /gene="yqcC"
                     /locus_tag="ACHKA1_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO5678314.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDVPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(22651..23196)
                     /gene="syd"
                     /locus_tag="ACHKA1_000613"
     CDS             complement(22651..23196)
                     /gene="syd"
                     /locus_tag="ACHKA1_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO5678315.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23264..24112
                     /gene="queF"
                     /locus_tag="ACHKA1_000614"
     CDS             23264..24112
                     /gene="queF"
                     /locus_tag="ACHKA1_000614"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO5678316.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFQGADIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGNVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            24224..25588
                     /gene="ppnN"
                     /locus_tag="ACHKA1_000615"
     CDS             24224..25588
                     /gene="ppnN"
                     /locus_tag="ACHKA1_000615"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO5678317.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKHTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            26145..27434
                     /gene="sdaC"
                     /locus_tag="ACHKA1_000616"
     CDS             26145..27434
                     /gene="sdaC"
                     /locus_tag="ACHKA1_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO5678318.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            27492..28859
                     /gene="sdaB"
                     /locus_tag="ACHKA1_000617"
     CDS             27492..28859
                     /gene="sdaB"
                     /locus_tag="ACHKA1_000617"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO5678319.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            28971..29726
                     /gene="xni"
                     /locus_tag="ACHKA1_000618"
     CDS             28971..29726
                     /gene="xni"
                     /locus_tag="ACHKA1_000618"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO5678320.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMLALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(29781..30929)
                     /gene="fucO"
                     /locus_tag="ACHKA1_000619"
     CDS             complement(29781..30929)
                     /gene="fucO"
                     /locus_tag="ACHKA1_000619"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO5678321.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIICNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(30957..31604)
                     /gene="fucA"
                     /locus_tag="ACHKA1_000620"
     CDS             complement(30957..31604)
                     /gene="fucA"
                     /locus_tag="ACHKA1_000620"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO5678322.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDTVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            32151..33467
                     /gene="fucP"
                     /locus_tag="ACHKA1_000621"
     CDS             32151..33467
                     /gene="fucP"
                     /locus_tag="ACHKA1_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO5678323.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            33500..35275
                     /gene="fucI"
                     /locus_tag="ACHKA1_000622"
     CDS             33500..35275
                     /gene="fucI"
                     /locus_tag="ACHKA1_000622"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO5678324.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            35384..36802
                     /gene="fucK"
                     /locus_tag="ACHKA1_000623"
     CDS             35384..36802
                     /gene="fucK"
                     /locus_tag="ACHKA1_000623"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO5678325.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGIGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            36804..37226
                     /gene="fucU"
                     /locus_tag="ACHKA1_000624"
     CDS             36804..37226
                     /gene="fucU"
                     /locus_tag="ACHKA1_000624"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO5678326.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            37284..38015
                     /gene="fucR"
                     /locus_tag="ACHKA1_000625"
     CDS             37284..38015
                     /gene="fucR"
                     /locus_tag="ACHKA1_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO5678327.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(38059..39159)
                     /gene="rlmM"
                     /locus_tag="ACHKA1_000626"
     CDS             complement(38059..39159)
                     /gene="rlmM"
                     /locus_tag="ACHKA1_000626"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO5678328.1"
                     /translation="MNKVVLLCRPGFEKECAAEISDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(39152..39547)
                     /gene="ygdD"
                     /locus_tag="ACHKA1_000627"
     CDS             complement(39152..39547)
                     /gene="ygdD"
                     /locus_tag="ACHKA1_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO5678329.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(39566..40483)
                     /gene="gcvA"
                     /locus_tag="ACHKA1_000628"
     CDS             complement(39566..40483)
                     /gene="gcvA"
                     /locus_tag="ACHKA1_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO5678330.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(40834..41061)
                     /gene="ygdI"
                     /locus_tag="ACHKA1_000629"
     CDS             complement(40834..41061)
                     /gene="ygdI"
                     /locus_tag="ACHKA1_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO5678331.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            41253..42458
                     /gene="csdA"
                     /locus_tag="ACHKA1_000630"
     CDS             41253..42458
                     /gene="csdA"
                     /locus_tag="ACHKA1_000630"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO5678332.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            42458..42901
                     /gene="csdE"
                     /locus_tag="ACHKA1_000631"
     CDS             42458..42901
                     /gene="csdE"
                     /locus_tag="ACHKA1_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO5678333.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRKTFAPLTQWEEKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAEHGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(42952..43758)
                     /gene="tcdA"
                     /locus_tag="ACHKA1_000632"
     CDS             complement(42952..43758)
                     /gene="tcdA"
                     /locus_tag="ACHKA1_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO5678334.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(43996..45093)
                     /gene="mltA"
                     /locus_tag="ACHKA1_000633"
     CDS             complement(43996..45093)
                     /gene="mltA"
                     /locus_tag="ACHKA1_000633"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO5678335.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            45302..45378
                     /locus_tag="ACHKA1_000634"
     tRNA            45302..45378
                     /locus_tag="ACHKA1_000634"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45336..45338,aa:Met,seq:cat)
     gene            complement(45452..46705)
                     /gene="amiC"
                     /locus_tag="ACHKA1_000635"
     CDS             complement(45452..46705)
                     /gene="amiC"
                     /locus_tag="ACHKA1_000635"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO5678336.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            46937..48268
                     /gene="argA"
                     /locus_tag="ACHKA1_000636"
     CDS             46937..48268
                     /gene="argA"
                     /locus_tag="ACHKA1_000636"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO5678337.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(48330..50156)
                     /gene="recD"
                     /locus_tag="ACHKA1_000637"
     CDS             complement(48330..50156)
                     /gene="recD"
                     /locus_tag="ACHKA1_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO5678338.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(50156..53698)
                     /gene="recB"
                     /locus_tag="ACHKA1_000638"
     CDS             complement(50156..53698)
                     /gene="recB"
                     /locus_tag="ACHKA1_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO5678339.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESRLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLSDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYSLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDVSTAELNAKTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VREKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSARNKQVEMEFYLPISEP
                     LIASQLDTLIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAELITLMDEMFAGMTLEEA"
     gene            complement(53691..56579)
                     /gene="ptrA"
                     /locus_tag="ACHKA1_000639"
     CDS             complement(53691..56579)
                     /gene="ptrA"
                     /locus_tag="ACHKA1_000639"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO5678340.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAASIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(56755..60123)
                     /gene="recC"
                     /locus_tag="ACHKA1_000640"
     CDS             complement(56755..60123)
                     /gene="recC"
                     /locus_tag="ACHKA1_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO5678341.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(60136..60459)
                     /gene="ppdC"
                     /locus_tag="ACHKA1_000641"
     CDS             complement(60136..60459)
                     /gene="ppdC"
                     /locus_tag="ACHKA1_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO5678342.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGRAGEMTRLH
                     CPNRQ"
     gene            complement(60444..60851)
                     /gene="ygdB"
                     /locus_tag="ACHKA1_000642"
     CDS             complement(60444..60851)
                     /gene="ygdB"
                     /locus_tag="ACHKA1_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO5678343.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMYSWQTQPAVQCLQYAGTDARVCLRLLADNEALLIAGYEGVSL
                     WRAGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(60848..61411)
                     /gene="ppdB"
                     /locus_tag="ACHKA1_000643"
     CDS             complement(60848..61411)
                     /gene="ppdB"
                     /locus_tag="ACHKA1_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO5678344.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(61402..61872)
                     /gene="ppdA"
                     /locus_tag="ACHKA1_000644"
     CDS             complement(61402..61872)
                     /gene="ppdA"
                     /locus_tag="ACHKA1_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO5678345.1"
                     /translation="MKTQRGYTLIETLVAMLILVTLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISFIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(62057..62851)
                     /gene="thyA"
                     /locus_tag="ACHKA1_000645"
     CDS             complement(62057..62851)
                     /gene="thyA"
                     /locus_tag="ACHKA1_000645"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO5678346.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(62858..63733)
                     /gene="lgt"
                     /locus_tag="ACHKA1_000646"
     CDS             complement(62858..63733)
                     /gene="lgt"
                     /locus_tag="ACHKA1_000646"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO5678347.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(63884..66130)
                     /gene="ptsP"
                     /locus_tag="ACHKA1_000647"
     CDS             complement(63884..66130)
                     /gene="ptsP"
                     /locus_tag="ACHKA1_000647"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5678348.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(66143..66673)
                     /gene="rppH"
                     /locus_tag="ACHKA1_000648"
     CDS             complement(66143..66673)
                     /gene="rppH"
                     /locus_tag="ACHKA1_000648"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO5678349.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(67026..67172)
                     /gene="ygdT"
                     /locus_tag="ACHKA1_000649"
     CDS             complement(67026..67172)
                     /gene="ygdT"
                     /locus_tag="ACHKA1_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO5678350.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFGGSVLNLVAHSVDK
                     YGIG"
     gene            67358..68047
                     /gene="mutH"
                     /locus_tag="ACHKA1_000650"
     CDS             67358..68047
                     /gene="mutH"
                     /locus_tag="ACHKA1_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO5678351.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            68116..68829
                     /gene="ygdQ"
                     /locus_tag="ACHKA1_000651"
     CDS             68116..68829
                     /gene="ygdQ"
                     /locus_tag="ACHKA1_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO5678352.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            68967..69185
                     /gene="ygdR"
                     /locus_tag="ACHKA1_000652"
     CDS             68967..69185
                     /gene="ygdR"
                     /locus_tag="ACHKA1_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO5678353.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            69293..70333
                     /gene="tas"
                     /locus_tag="ACHKA1_000653"
     CDS             69293..70333
                     /gene="tas"
                     /locus_tag="ACHKA1_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO5678354.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(70365..71558)
                     /gene="lplT"
                     /locus_tag="ACHKA1_000654"
     CDS             complement(70365..71558)
                     /gene="lplT"
                     /locus_tag="ACHKA1_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO5678355.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(71551..73710)
                     /gene="aas"
                     /locus_tag="ACHKA1_000655"
     CDS             complement(71551..73710)
                     /gene="aas"
                     /locus_tag="ACHKA1_000655"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO5678356.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            74296..75327
                     /gene="galR"
                     /locus_tag="ACHKA1_000656"
     CDS             74296..75327
                     /gene="galR"
                     /locus_tag="ACHKA1_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO5678357.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(75334..76596)
                     /gene="lysA"
                     /locus_tag="ACHKA1_000657"
     CDS             complement(75334..76596)
                     /gene="lysA"
                     /locus_tag="ACHKA1_000657"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO5678358.1"
                     /translation="MPHSLFSTDTALTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            76718..77653
                     /gene="lysR"
                     /locus_tag="ACHKA1_000658"
     CDS             76718..77653
                     /gene="lysR"
                     /locus_tag="ACHKA1_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO5678359.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(77640..78332)
                     /gene="ygeA"
                     /locus_tag="ACHKA1_000659"
     CDS             complement(77640..78332)
                     /gene="ygeA"
                     /locus_tag="ACHKA1_000659"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO5678360.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEELSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(78461..79879)
                     /gene="araE"
                     /locus_tag="ACHKA1_000660"
     CDS             complement(78461..79879)
                     /gene="araE"
                     /locus_tag="ACHKA1_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO5678361.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(80194..80955)
                     /gene="kduD"
                     /locus_tag="ACHKA1_000661"
     CDS             complement(80194..80955)
                     /gene="kduD"
                     /locus_tag="ACHKA1_000661"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO5678362.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(80985..81821)
                     /gene="kduI"
                     /locus_tag="ACHKA1_000662"
     CDS             complement(80985..81821)
                     /gene="kduI"
                     /locus_tag="ACHKA1_000662"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO5678363.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVTDEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASDDTATPAKFYYNCVPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(82108..83289)
                     /gene="yqeF"
                     /locus_tag="ACHKA1_000663"
     CDS             complement(82108..83289)
                     /gene="yqeF"
                     /locus_tag="ACHKA1_000663"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO5678364.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVIDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            83544..84773
                     /gene="yqeG"
                     /locus_tag="ACHKA1_000664"
     CDS             83544..84773
                     /gene="yqeG"
                     /locus_tag="ACHKA1_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5678365.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT        20998 a        22693 c        20658 g        20781 t
ORIGIN      
        1 ggtttttcag agacgacaaa gaatacaaat gagtggaatt ctttacaagc tccacccatt
       61 tataacgctt ataaatgttt aatcaggcaa tatttaaata tttatgggtt tgcatcgaca
      121 aacgccaatt acgcgcaatg caggtttcaa tgcacaaacg tgtggcatca tccttttggc
      181 taatcggctg cagtgcaatg actcgcggtt tatcatcggt cagcgtcgcc aaaagttcat
      241 ccagtgcttc aatatcacgt acgcgtccca ctggatgctt gatttcgttg gctcgctcca
      301 gtgcctgtga caacacttca tagccgccgc gcatgttcag ctttggcgat acggtaaccc
      361 aggtattcgg tgtacagcgt acttcatgag taccactggt ttcaatctgg cagctaaaac
      421 cgttcttttc gagcaggtca gtcagtggca gcaaatcatg aatgcaaggc tcaccacccg
      481 taatcaccac atgccgcgcg gtatatccct ggcgaccaat gacagccagc aagtcttcac
      541 tgctcgccgc cccccactta tcactctctt tggtcttcgc cagaatgctg aaaagggaga
      601 cttcccgatc ctcaagcttt tcccaggtgt gtttggtgtc gcaccaggca cagccaaccg
      661 ggcatccctg taaacgaata aaaatggcgg ggacgccggt aaagtaaccc tcaccttgca
      721 gggtctggaa catctcgtta atcgggtact gcatagcatt ctctgtgaag tggataattg
      781 ttaattattg cagatcctgc cacaacaatc atgtcttatt aacattctgt tacaggcagg
      841 tttatggcaa aaagtggatt agatcataaa gaataaaatg aagcagagta ttatgtttta
      901 aggaattaat taaaagggat ttaaacatgc tcagaatatt catcgtaatt atatttattt
      961 tcaatttatc aggctgtgga tataacgata ttcaaaccta tgatgagcag gttaacgcct
     1021 cctggtctga ggtattgaat caatatcaac gtcgcgccga tcttattcct aatcttgtcg
     1081 ccagcattaa aggttattcc agccatgaac gagatgtact ggaagccgta acattggctc
     1141 gtagccaggc taatcgcgcc agcagcgatc ttcagcaaac tccgggagat gaacaaaaat
     1201 tacaagcctg gcaacaggct caagcgcagg tgactcgcac tctcggtcag ttaacgataa
     1261 ttagtgagcg ttaccctgag ttaaaatctc aggaactcta tcaaaattta atggtacagc
     1321 ttgaaggaag tgaaaatcgg attgccgttg cgcgaggaag atatattaaa gccattgaac
     1381 aatataatgt cactatccgc aaattccccg ctgtgttaac cgcaaaggtc atggattaca
     1441 caccaaagaa aaattatttg ccggatgatg tcgccgcagt aagtaaagcc ccaacaatag
     1501 attttagcca gaatgctaat gctcattaag cggggaagtt ctatgcgaaa ttttatcttc
     1561 cttatggctt tcttctgttc atctgtgttt gccacacaaa ttccagtacc tgaatcaccc
     1621 aagtatgtga atgacttaac gggcacatta acaaacagcg aagtaaacac cctgaccaac
     1681 cagattaaag ccctgacgca aaaaagccac gcacaactgg tcgtgttatt ggttgaaaca
     1741 acaggcgatg aaaccatcga gcagtacgca acgcgggtat tcgatagctg gaaaccgggt
     1801 gataaagacc gcgatgatgg cgttctactg ttagtcgcct ggcaggatca taccgtgcgt
     1861 attgaaatcg gttacggact ggaaggtatt attacggatg cgcagtccgg gaaaattatt
     1921 cgcaacagta ttattcctgc atttaaaaaa ggcgacctgg ctggcggttt acaaaagggg
     1981 atcagcgata ttgaaagtcg tcttacgggg aataatttag cgacgatagc accggccgat
     2041 caccctttac cttttagcgg ttggtgggcc ttgttagcct gggcgatagt actgacgttt
     2101 atttctgcca gaggatatat caaaacgctg ggagtaattt gcttcgcagc tattgtactg
     2161 gcctttgttt tacctatagc aggtttcagc ggaagctggg gagtactcac aacattactc
     2221 tgcttcgcca caccgttcct ggctgtcgcc atcgctttta cccccttcgg caaaaaagtt
     2281 cgggatttga tgcgtgatgc caaccagcca tcacgccata caagaagcaa ttcttcctcc
     2341 cactattcat catcgtcatc ctcttcattc aacaatgaca atttcagcgg tggtggcggt
     2401 tcatctggtg gcggtggcgc ttccgggcgc tggtaacaaa aggaattgat atgcgtattt
     2461 ttctgttact tctgatgcta tgcagttttc aggtattcgc tgaacgattg ccttcagtga
     2521 atgacttaac cgggacatta acgacagaag aacaaagtgt tttaaatcaa caattacaga
     2581 ctctcgagca gcaaaaccac ttacaggtgg ccgtgttagt tacgccaacc acagggggca
     2641 aaaacattaa acttgcagcc tcgcagagct acgggttata tcactggaaa cccattggag
     2701 agaagcgata cggcgaaggt atcttaattc tcgtcgtttg gccggaaggg ctggcgtcaa
     2761 tgaagattgg acatggactg gaagagatgt tgccgccgaa acaagctgcg caaattgttc
     2821 gttaccatat gcagccagaa ttcgaaaaaa acaatctctt tgctggatta acgggcggaa
     2881 ttgaaagcat cgcacagttt acccatatca aagcgaacct tagccctttg gatgctttag
     2941 ccaatcatct gtttgctaat ccgcaacgtt cgctgccatg tcttgcctgg acactgctga
     3001 tgatcgttgc catcgttgtc ctctggcgat ttgccagccg cccacggcgt ggcatatgga
     3061 tgatatcgat gattacccct atggtctggt ttttctcctt tcaggacgat gtcatcattc
     3121 ggcgggtatc ggtcgtttgt tttagtttat tgttagcaac aacctgcaag cagcgtctcg
     3181 cggtagtttt taatatgtgc cgcgtatttc ctatgatgct ggccccaaaa aataaaaacg
     3241 ctaaagtgaa aaaacaaaag aagactccgc aggttcgtga atcaggacga aattcattgt
     3301 ttgtcgtgat gctgataatt attgtgggat ggtgcctggt gttcgccagt attaaagata
     3361 ttgcattcat ctcaacaatt attgggttaa ttgatcattt tatcggtcca ataaccattg
     3421 ctggcatcgt tctgctgatc attgtggcca aatttactgg caatcttaag ctgggatcag
     3481 gagaaaaaag caacaggaaa acggggaatc gtaatgctca ctctcgctct tcgtcgagaa
     3541 attctttccg cggtggtggc ggttcttcag gaggcggcgg ttcatccggc cgttggtgat
     3601 cgtaatgcgc ttaatcttta ttctacttgc tttgtggagc cttcccgggc ttgcccagca
     3661 aattgctgtg ccggagctgc gccaacaagt gactgatatt actggtacgt taagaacgtc
     3721 tgaacaacaa tcactgacac agcagctgca ggatattaca cataaaacac gggcgcaggt
     3781 cgctgtatta gtagtgccgt ccaccggaga cgattccatt gaacaatatg ctacaagggt
     3841 ttttgatagc tggaaactgg gagataagca gagaaacgat ggtattttac ttctggttgc
     3901 ctgggaggat catgctgttc gtattgaggt tggctacggg ctggaagggg tggttaccga
     3961 cctgcaagcg gcgaagatta tcagggatat attgatccct gccttcaaaa gtgatgacct
     4021 gatgggtgga ttaacactgg caagcgaaaa tatcggcgcg cttctgttga atggtgaatt
     4081 accggaagac aggggggatt attacagtat caatccccct attccattat cgcttgctgt
     4141 aattatattg ctggcggtac tttcatattt tattgttttt accgatccgt cgaatttacc
     4201 ctggatcact ctcactggcg ccatttacgg gatggtattc ctctatgttg ccgagccagg
     4261 accatggacc aatttaatcg ttgcctgcgg tatgttaacg ccttttgcga tcgtaccact
     4321 ggttattttt tggcttatcg tcaataaaaa gctacgcgcc aaatacaaaa aactcagtaa
     4381 agaaagagct tcaagaaaag gttcttcttc atcctcttcc ggtggaggct caagtggcgg
     4441 cggatttagc ggcgggggcg gttcttctgg aggtggcggc gcatccggcc gctggtaact
     4501 caaccataaa aaatgccagc ctccgggctg gcatttttaa atcagataaa gtcagactta
     4561 tgcctggcct ttgatctctt tacgaccgtt gtacggtgct ttttcgccca gagcttcttc
     4621 gatacgaatc agctggttgt atttagcaac gcggtcagaa cggctcatag aaccagtttt
     4681 gatctggcct gcagcagtac caacagccag gtcagcgatg gtagcgtctt cagtttcgcc
     4741 agaacggtga gagataactg cagtgtagcc agcgtctttc gccatcttga ttgcagccag
     4801 agtttcggtc agagaaccga tctggttgaa tttgatcagg atggagttag cgataccttt
     4861 ttcgatacct tctttcagga tcttggtgtt ggttacgaac aggtcgtcac caaccagctg
     4921 gattttgtcg cccagaactt tggtctggta tgcgaaaccg tcccagtcag attcgtccag
     4981 accgtcttcg atagaaacga tcgggtactg tttggtcagt tcttccagga agtgagtgaa
     5041 ttcttcagag gtgaaagctt tgttgccttc gccagccaga acgtatttac cgtctttgta
     5101 gaattcagaa gccgcgcagt ccatcgccag agtgatgtct ttgcccagtt cataaccagc
     5161 agctttaaca gcttcagcga taacagccag agcttcagcg ttggaaccca ggttcggcgc
     5221 atagccacct tcgtcaccaa ctgcagtgtt catgcctttc gctttcagaa cttttgccag
     5281 atgatggaaa acttcagaac ccatgcggat ggcttctttc acagttttcg cgccaaccgg
     5341 ctgaatcatg aattcctgga tatcgacgtt gttgtcagcg tgctcaccac cgttgatgat
     5401 gttcatcatc ggaaccggca tggagtattt gcccggagtg ccgttcagtt cagcgatgtg
     5461 ctcgtacagc ggcatacctt tagcagctgc agcagctttg gcgttagcca gagatacagc
     5521 caggattgcg ttcgcgccga atttggattt gttttcggtg ccgtccaggt cgatcatgat
     5581 cttgtcaatg ccagcctgat ctttagcatc tttgccaatc agcgcctgag cgatcgggcc
     5641 gtttaccgca gcaacagctt tggttacgcc tttacccagg aaacgggatt tgtcgccatc
     5701 gcgcagttcc agagcttcac gggaaccagt agaagcacct gacggagcag ctgccatacc
     5761 gacgaaacca ccctccagat gtacttcggc ttcaacagtc gggttaccac gggagtcgat
     5821 gatttcacga ccgatgattt ttacgatttt ggacattagg ttttcctcaa gtcactagtt
     5881 aaactgaaac tccagacaaa caacgcgtac ccagggtacg cgttgccgtt ctaacttttt
     5941 tacttacttc gcctgacgtt tctggaactc gctggcggct ttcacaaagc ctgcaaacag
     6001 cgggtgacca tcacgtggag tagaagtaaa ctccggatgg aactggcaag ccacgaacca
     6061 cgggtgattc ggaacttcga tgatctcgac caactgatca tccccggaac ggcccgcaac
     6121 gcgcagacct gcatcttcaa tctgtttcaa cagcatgttg ttgacttcgt aacggtgacg
     6181 atgacgctca acaattgtcg gcgcattgta cagctggcga accaagctat cgtcaaccaa
     6241 ctggcactgc tgtgcgccga gacgcatggt accgccgaga tcgctcttct cgctacgaac
     6301 ttcaacgttg ccgttttcat cgcgccactc ggtaatcagc gccacaaccg ggtacttaca
     6361 gtctggcaca aattccgtag agttggcgtt ctccatgttg gcaacatggc gagcgtaatc
     6421 aattaacgcc acctgcatac ccaggcaaat gcccagataa ggaatattgt tctcacgcgc
     6481 aaaacgcgcg gtcgtaatca tgccttctac gccacgatag ccgaaaccgc caggtacgag
     6541 gattgcgtcc agacctttaa ggatttcaac gccgcgcgtt tcaacatctt gtgaatcgat
     6601 cagtttgatg ttgacgctga cacgattctt cagcccaccg tgtttcagtg cttcgatcac
     6661 ggatttataa gcatccggca gttcaatgta cttgccgacc ataccgatgg tgacttcact
     6721 taccggattc gcttcttcga agataacctg ttcccattcg gacagattcg cttccgggca
     6781 gtttaagctg aatcgtttac aaatataatc gtccagcccc tgagatttca acaggcccgg
     6841 aattttatag atggaatcga cgtctttcag agaaataacc gctttttccg gaacattaca
     6901 gaacaatgca atcttcgcac gttcgttcgc cggaacggcg cgatctgaac gacaaatcag
     6961 gatgtcaggc tggataccga tggagagcag ctcttttaca gagtgctgag tcggtttggt
     7021 tttgacttca ccagacgctg ccatgtacgg caccagcgtc aggtgcataa acagagtgtg
     7081 ctcacggcca atttcaacag ccatctggcg aatcgcttcg aggaacggca gggattcgat
     7141 atcacctact gtaccgccga tttcgaccag tactacgtca tgaccttcgc caccttccag
     7201 cacgcgctct ttgattgcgt tagtgatgtg cggaataacc tgtacggttg cgccgaggta
     7261 gtcaccgcgg cgttctttac gcagaacgtc agagtagata cgacccgtgg tgaagttgtt
     7321 gcggcggctc attttggtac ggatgaaacg ctcgtagtgc cccaggtcca ggtcggtttc
     7381 agcgccgtct tcagtaacga acacttcccc gtgttggatt gggctcatag tacctggatc
     7441 gacgttgatg tacggatcca gtttcatgat ggtcacattg aggccacggg cttcaagaat
     7501 ggctgcgagg gaggctgcgg caatgccttt acccagagag gatacgaccc cgccggtcac
     7561 aaaaatatag ttcgttgtca tgctgaacct gagaagttag gttgaaaaga cgatggaata
     7621 accaggacgg gaaagcagta tacccgaaca tgacctgtgc cacaaacttt cattatccct
     7681 cctcttcgcc agcgcactat tgaaatcagg agtgagaaaa tagccccttt ggggtaaatg
     7741 tttttgacgc aaatcaagcg cttgtcattt aaaaaatgac acaaatggcg cttgaccgcg
     7801 taattccctt agagatcaat ttcctgccgt tttacctgtt gccagacttc ttccattgtt
     7861 tcgaggtcaa cacctgtcat ttccagtcca cgcgcggcaa caatacgctc cacttcgcga
     7921 aaacgacgct cgaatttttc gttcgctttt tgcaatgcga tttccgcttt tgtccctaaa
     7981 tgacgagcca gattgaccgt ggcaaacagc aggtcaccca tttcctcctc cagtttagcc
     8041 tggtcgataa cagcctgccg tgcctcgtac atcacctcgt cgatctcttc gtagacttta
     8101 tcaaccactg ggccaagcgt cgtccagtcg aagccaacgt tggcgcaacg tttctggatt
     8161 ttttgcgccc gcattaaagc cggtaaacta cgaggaatat cgtccagcgc cgaatgctgc
     8221 gctttctgcg cgcgctcttc ggttttgatt tgctcccaac gggcaagcac ttcactactg
     8281 ttttcggcag aactgtcagc aaaaacatgc ggatggcgac gctctaattt atcgctaatc
     8341 gcagcgcaaa tatcattaaa gtcaaagcgc ccttcttcct gagccatttg ggcgtaaaac
     8401 accacctgga acagcagatc gccgagttca ccacgcagat cgtcaaaatc ttcacgggcg
     8461 atggcgtcca gcacttcgta ggtttcttca agggtgtaag gcgcaatggt ggcaaatgtc
     8521 tgctctttat cccacgggca gccgttttcc gggtcgcgca ggcgctgcat aatagtgagc
     8581 aaacggtcga tttgattcat tagaatgtcc tgaaaattgc gggtccgtca ggtggaaacc
     8641 tgtgaccaga atagaagtga gttagtaaca ctacccaatc agtacgttaa ttttggcttt
     8701 aatgagttgt aattcctctg gggcaactgt tcctttcttc gttgctcctc ttgcccgcca
     8761 ggcgatactt tttacctgat cagctaacgc tacgccatca cgttcctgac cggataaaac
     8821 aacttcgaac ggatatcctt ttgattgcgt tgtacaagga acacacagac acatacctgt
     8881 tttgttgttg tacatgaacg gactcaggac aacagccgga cgatgtccgg cttgctcgct
     8941 accttttgtc gggtcaaaat caacccaaat cagatcgccc atatcgggta cgtatcggct
     9001 taccattacc agacttcctt atctttcggc tctccccagt cgatattctc gtggaggttt
     9061 tccggcgtga tgccgttgac cagttcagca agcgtaaata cgggctcttt acgcactggc
     9121 tcaataatta atttgccatc caccaggtca atcttcactt catcatcaat attcagattg
     9181 agcgcctgca ttaacgtagc cgggatccgc accgccggtg aatttcccca acgctttacg
     9241 ctactgtgga tcataaccct ttcctcaaac cgctatcata tgtagataca gtatatatca
     9301 atctacattg tagatacgag caaatttcgg cctaactccc gtgcaaccga cgcgcgtcga
     9361 taacatccgg cacctggttg agtttaccca gcacgcgacc cagcacctgc aggttgtaaa
     9421 tctcaatggt catgtcgatg gtcgccagtt gctgtttggt gtcgctacgg ctggcaacgc
     9481 caagcacgtt caccttctcg ttggcgagaa tggtcgtgat atcacgtaac aacccactac
     9541 gatcattagc caccacgcgg accaccagcg aatatccggc ggagtagctc tcaccccata
     9601 ccgcgtcaac aatgcgttct ggcgcatggg agcgcagttc cgccagttgt tcgcaatcgg
     9661 cgcggtgtac tgaaataccg cgcccctggg ttatgaagcc gacaatctca tctccaggaa
     9721 tcggctggca gcagcgcgcg atgtggtgca tcaggttacc gacaccttca accaccacac
     9781 gaccgttatc tttactgcgg ttttgcggtg tgtagctttt ttgctgaagc tgtttcagcg
     9841 cggcggcgtc ctgctcttcg gcactcggct tattaaattg cgattgcagg aagttcacca
     9901 tctgattgag acggatatcc ccgccaccaa tcgccgccag caactcgtcg acatcattga
     9961 agttgtaacg cggcagcaga tgtttttctg cttctttcag gctgatcccc agatgttcca
    10021 gctcgtcgtc aaggatttgc cgcccagcca gaatgttttt gtcacggtcc tgtttacgga
    10081 accaggcgtg aattttcgaa cgcccacggc tggttgtgac gtaaccgagg tttgggttta
    10141 accagtcacg gctggggttc ggctgtttct gggtgataat ttcaatctgg tcgcccattt
    10201 gcagctggta ggtgaacggc acaatgcgcc cgccaatttt tgccccgata cagcggtgtc
    10261 cgacatcact gtggatgtgg taagcgaagt ccagcggcgt tgatcccgca ggcaaatcaa
    10321 cgacatcacc tttcggcgta aagacataca cccggtcgtc aaacacctga ctacgtactt
    10381 cgtcgagcat ttcgccggaa tcagccatct cttcctgcca cgcaatcagt ttacgcagcc
    10441 aggcaatccg gtcttcatgt cccgaacgtg cgccgccagc tgccgcgccc tctttatatt
    10501 tccagtgcgc agcaacaccc aactctgcat cttcatgcat ctgtttggtg cggatttgga
    10561 tctcaacggt ttttccaccc ggccccagaa ccacggtatg aatagactga taaccgtttg
    10621 gtttcgggtt agcgacgtaa tcatcaaact catccggcag gtggcgatag tgtgtgtgca
    10681 ctatccccag tgcggcataa caatcctgta aacgctcggc gacaatacgt accgcacgca
    10741 catcaaacag ctcatcgaag gcgaggttct ttttctgcat tttgcgccag atgctgtaga
    10801 tgtgtttcgg acgaccatac acttcagctt taacgccttc agctttcatc tcagcgcgca
    10861 gatgaccgac aaactcttcg atatagtgtt cgcggtcgag acggcgttca tgcaacagtt
    10921 ttgcgatgcg tttgtattcg gtcgggtgga gataacggaa gcagtaatct tccagctccc
    10981 atttcagttg cccaatccca agacggttcg ccaacggcgc gtagatattg gtgcactctt
    11041 ttgccgccag tacgcgttca tcttccggcg catcttttac ttcacgcaga tgagcaatac
    11101 gctccgccag tttgatgacc acgcagcgaa aatcatcgac catcgccaat aacatccggc
    11161 gaacgttatc gacctgttcg gaggaaacag aatcagtgtg cgtcgctttc agctggcgga
    11221 tcgccgccat atcacgcacg ccgtgaataa ggttaacgac cgacttaccg acgctctcac
    11281 gcagcacatc ttcgctgact acgttggcat ccgccagagg gaaaagcagc gccgcccgca
    11341 gcgtgtcaat gtccatactt aatgtcgaga ggatctccac catctcaaca ccacgccaca
    11401 ataacagact ggcatccgga tgcccctgcg tctgttgcag acaatacgcc caggtttcgg
    11461 ctaagcactc acacgacttc tggctggtaa tacccagact tgcgatccat ttttccggat
    11521 caaattcacc agccttattg atatgtgcac ttcttaccgc aaccatcgtc ctctccttta
    11581 gggaccagac ctgccgaaat cggcaaatcg caactattta acgcgcgaga aaagtaccat
    11641 cgattccaga tgtcccgtgt gtgggaacat atccagcatc gccagtcgcg caatggtata
    11701 tcctgctttt aataacgctt cgctatcccg agccagcgtt gcagggttac aggatacata
    11761 aactatacga ataggttcca gttttataat ttgctgcata acacctgcgg cacctgctcg
    11821 cgcggggtcc agcaacactt tatcgaagcc gtttttcgcc cacggctgct ttgtgacatc
    11881 ttcttcaaga ttttcatgat aaaacgtcac attctgtaag ccattaagac gcgcattctg
    11941 ctggcctttt tccaccagcg caggaacacc ttctacaccc accacactgg ctgcttgtgt
    12001 cgccaatggc agtgtaaagt tgcccatacc gcagaacaga tccagtacgc gatcttcagg
    12061 ttgcacatcc agccattcca acgcacgcgc taccattttt tggttcacac ccgcattgac
    12121 ctgaataaaa tcgcgcgggc taaaagttaa gcgcaaccca tttgagtcat accagggcat
    12181 ctcaccagag acggtttcga gtatctcact atcgggggcg agatacagat ccaggccttc
    12241 agaatgcgaa aagcgttcca gtttttcgcg atctgccgaa cttagcggtg cggtatggcg
    12301 caaaatcatc agcgtgccgc tggttgcctg taccagttca acatgaccaa gatggcgcat
    12361 agcttgtaag ctgcccaggc atgccctgac tttgggcagc aatgcttcaa gttggggcac
    12421 taaaatgggg cattgtttaa cgtcgacaat gtcactggag cccgctttgc gaaaccccat
    12481 ctgaagttgc tgtgttttcg gtaagtaatt cagacttaaa cgcgcgcgac ggcgatagcc
    12541 ccagggaaca tcggcgatca cttcagagac ttcgtgtttc attaatcggg cgagtgccgc
    12601 acttttgctt cgctgctgta aatccacgct ggcgtgttgt tgctgacagc cgccgcatac
    12661 gccaaaatga ggacagcgtg gcgtttcgcg ttccgggcta tcgcttaacc ggcgtacgac
    12721 tttagcgcgg gaatactgtt ttttatcttc agtaacagta acttccgcgt tttcctgcgg
    12781 caataatccg gggataaata gcgttttgcc gttatgtcgc gccacgccct gaccaaaaga
    12841 gtcgaggtcg ttgactgaaa cggttatgat ctgacgcgtc gtcgtgcgtc gttttgcaga
    12901 gtagaattgc gccattggcg agactttctc aatttaacag tgtgacctta attgtcccat
    12961 aacggaactc catgaccaac tacagcctgc gcgcacgcat gatgattctg atcctggcac
    13021 cgaccgtcct tattggttta ttgctgagta tctttttcgt cgtgcatcgc tataacgact
    13081 tgcagcgtca actggaagat gccggtgcca gcattattga gccgcttgca gtttctactg
    13141 aatatggcat gagcctgcaa aatcgcgaat ctatcggtca gttaataagc gtactgcatc
    13201 gtcgccattc cgatattgtt cgcgcgattt cggtttatga tgaaaataac cgactctttg
    13261 tcacctccaa ttttcatctt gatccctcat caatgcagct cggcagcaac gtgccgtttc
    13321 ctcgccagct cactgtcact cgtgacggcg atattatgat cctccgcacg ccgattattt
    13381 ctgagagtta ctcccctgac gaatcgccca gtagcgatgc caaaaatagt caaaatatgt
    13441 tgggatatat tgcgcttgag ctggatctta aatcggttcg cttgcagcaa tataaagaga
    13501 tctttatttc cagcgtgatg atgctgtttt gtatcggtat tgcgcttatt tttggctggc
    13561 gcttaatgcg cgatgtaacc ggtccgattc gcaacatggt gaataccgtc gaccgcatcc
    13621 gtcgcgggca actcgacagc cgggtggaag gatttatgct cggcgagctg gatatgctga
    13681 aaaacggtat caactcgatg gcaatgtcgc tggctgctta tcacgaagag atgcagcaca
    13741 atattgacca ggcgacgtcc gatctgcgtg aaacgctgga gcagatggaa attcagaacg
    13801 ttgagttgga tctggcgaaa aagcgcgccc aggaagcggc gcgtattaaa tccgagtttc
    13861 tggctaatat gtcacacgag ctgcgtacac cactgaatgg tgttattggc tttacccgcc
    13921 tgacgctgaa aacagaatta acaccaacgc agcgcgatca cctgaatacg attgaacgtt
    13981 cggcaaataa tttgctggca ataattaatg atgttctcga cttctcaaaa ctggaagcag
    14041 gtaagctgat tctggaaagt attccattcc cactacgcag cacgctggat gaagtcgtta
    14101 ccctgctggc tcattcttct cacgataaag ggctggaact gacgctcaat attaagagcg
    14161 acgtgcctga taacgtgatc ggcgacccac tgcgattaca gcaaatcatc actaacctgg
    14221 tggggaatgc aattaaattc accgagaatg gcaacattga tattctggta gaaaaacgtg
    14281 cgctgagtaa taccaaagtg cagattgaag tgcagattcg ggataccggc attggtattc
    14341 ctgaacgcga tcaatcgcgc ttattccagg ccttccgaca ggctgatgcc agtatttccc
    14401 gccgtcatgg tggcaccggg ctggggctgg tgattacaca aaaactggtt aatgaaatgg
    14461 gcggcgatat ttcgttccat agccagccaa atcgcggttc aactttctgg ttccacatta
    14521 atctcgatct gaacccgaac attattattg aagggccatc cacccagtgc ctcgccggta
    14581 aacgcctggc ctatgtcgaa ccaaactccg cagcagcgca atgcacgctg gatattttaa
    14641 gtgaaacgcc gctggaagtg gtttatagcc caacgttctc cgcgctgcct cccgcgcatt
    14701 acgacatgat gttgttaggc atcgcggtga ccttccgcga gccgttaaca atgcaacatg
    14761 agcgattagc gaaagcggta tcgatgaccg atttcctgat gctggcactt ccttgccatg
    14821 cacaagtcaa tgctgaaaaa ctcaagcaag atggtatcgg cgcgtgtctg ctgaaaccat
    14881 taacacctac gcgcctgctg cctgccctga cggaattttg tcatcacaaa caaaacacgc
    14941 ttttgcctgt aaccgatgaa agtaagctgg caatgacagt catggcggtt gatgacaacc
    15001 ccgctaacct gaaacttatc ggcgcattgc tggaagatat ggtgcaacat gtggaacttt
    15061 gcgatagcgg gcatcaggcg gttgaacggg cgaaacagat gccgttcgat ttgatcttaa
    15121 tggatattca aatgccggac atggatggca ttcgggcctg cgagctcatc caccagctcc
    15181 cccatcagca acaaacgccg gttatcgcgg taacggcgca tgcaatggcc gggcaaaaag
    15241 agaagctgct tggcgcagga atgagcgatt atctggcgaa accgattgaa gaagagcgat
    15301 tgcataattt gttgttgcgc tacaagcctg gcagcggtat ttcgtctcgc gtcgtgacgc
    15361 ccgaagtcaa cgaaattgtg gtgaacccga atgcgaccct cgactggcaa ctggcactac
    15421 gccaggcagc aggaaaaacc gatttagcgc gcgatatgct gcaaatgtta ctcgatttcc
    15481 tgcctgaagt tcgcaacaaa gttgaggaac agctggttgg agaaaacccg gaaggcctgg
    15541 ttgatttgat tcataaactg catggcagtt gcggctatag cggtgtgccg cgtatgaaga
    15601 atctctgcca actgatcgaa caacagctac gtagcggtac taaagaagaa gatttggagc
    15661 cggagctgct ggaactgttg gacgagatgg ataatgtcgc gcgcgaagcc agcaaaattc
    15721 tcgggtaacg gacgtcttgt cgagtagcaa tcggcttttt atgacgctgg tttcagattg
    15781 gtgacaaagt gcctgtgttt atgccggatg cggcgttaac gccttatccg gcctacaaaa
    15841 ccaattaaat tcaataaatt aaaaagcatg taggcctgat aagcgtagcg catcaggcaa
    15901 ttttgtgttt ggcatcagtc tcaaaccggc tcctgacaga gccggttttg gttttctgtc
    15961 ttaacgcacc atgcacgggc gcttgttatc gaacgtccag ccaggaatca gatactgcat
    16021 tcccatcgca tcgtcacgcg cgccaagccc gtgtttctga tacagctcat gggctttcat
    16081 cacttgatcc atatcgattt ctacacccag ccccggtttt tctggcacct gtaccagccc
    16141 gcctttgatc tcaaacggtt ctttggtcag gcgctgattg ccttcctgcc aaatccagtg
    16201 cgtatcaata gcagtaattt tacccggtgc agcggcggca acatgggtaa acatcgccag
    16261 ggaaatatcg aagtggttgt tagagtgtga accccaggtc aggccaaatt catggcacat
    16321 ttgcgccaca cgtaccgaac cttgcatcgt ccagaaatgc ggatccgcca gcgggatatc
    16381 aacggattgt aacgagagcg tatggcccat ctgccgccag tcggtggcga tcatattggt
    16441 tgcagtcggc aggccagtcg cgcgacggaa ctctgccatc acttcacgcc cggagaaacc
    16501 ttgctccgca ccacacggat cttctgcata agccagcgaa cctttcaggt atttaccaat
    16561 tttaatcgct tcgttcagcg accaggcacc gttaggatcg agcgtaatac gcgcctgcgg
    16621 gaagcgttgc gccagtgcca caatagactc ggcctcttct tccccggcca gtacaccgcc
    16681 tttcagtttg aaatcgttga aaccatattt ttcatacgcc gcttccgcca ggcgcaccac
    16741 cgcatccggc gtcatcgctt cttcatgacg caggcgatac cagtcgcatg agtcatccgg
    16801 ctggctttga tacggcagcg gtgtggcttt gcgattaccg acgaagaaca gataaccgag
    16861 catttcgact tcgctacgct gttgaccatc gcccagcagc gatgccacgt ttacccccag
    16921 atgctgcccc agcagatcca gcattgccgc ttctatcccg gtaactacat gaatagtggt
    16981 acgcaaatca aatgtctgca aaccgcgccc gccggcatca cgatcggcaa aagtattacg
    17041 caccagcgtc agaacgtttt tgtattcacc cagcgtttta cccactacca gcggaatcgc
    17101 atcttccagc gttttacgga ttttctcgcc gccgggaatt tcccctacgc cagtgtgacc
    17161 agaattatct ttgataatca caatattacg cgtgaagaac ggtgcgtgtg caccactcag
    17221 attcatcagc atactgtcat ggcccgccac cgggatgacc tgcatttcag taacaacagg
    17281 cgtcgtaaat tgagaactca tatttatgtc ctttttcaga atcaatgacg gccgaaaacg
    17341 ggacgtttac ggtcaaaggt ccagccgggg atcaggtact gcatcgggcc tgcgtcgtta
    17401 cgcgcaccgc caggcagacg tttataggcc tcatgtgcct tttgtacctg ttcccagtcc
    17461 agttccacgc ccagaccggg cgcatcagga acggcaattt ttccgttttt aatctccagc
    17521 ggattttggg tcaggcgaca atcgccctcc tgccaaatcc agtgggtatc gatagcggta
    17581 ggattacccg gcgccgccgc gcccacatgg gtaaacatcg ccagagagat atcgaaatgg
    17641 ttattagaat ggcagcccca ggtcagcccc cagtcgtcgc aaagctgcgc cacacggact
    17701 gcaccggaaa gcgtccagaa gtgcggatcg gcaagtggaa tatctaccgc attgagcatc
    17761 accgcatgac ccatttcgcg ccagttggtg gcgatcatgt tagtcgcgac gggcaagccg
    17821 gtcgcccgcc gaaactccgc catcacttca cgtccggaga agccctgttc tgcgccgcat
    17881 ggatcttcgg cataggtaag aacatcattc agccctttgc acagagaaat ggcttcatca
    17941 agcagccatg caccgttggg atcaacggta atccgcgcat ccgggaagcg tttcttcaat
    18001 gcacgaacag tgtcgatttc ttgctcgcca ggtaacacgc cgcccttaag tttaaaatct
    18061 ttaaagccgt agcgatcctg tgaggcttcc gccagacgca caacggcttc gctgttcatc
    18121 gctttctgat gccgcaactg ataccactca tggttgcccg gcgtattttc cagataagga
    18181 agatcggttt tggtccgatc accgatataa aacagataac cgaggacggt aatagcctcg
    18241 cgttgcttgc ctggccctaa cagttcgcag accggaacat tcagcgcctt acctaacagg
    18301 tcaagcaagg cggcttccag cgccgctacg gcattaacgc gcaattcaaa agtccaggca
    18361 cctttgccga aggtatcaaa atcggctgct tgattacctt tatgcacctg ctgaaccact
    18421 ttattcaggc gcgcgacttc ctggcccagc accatcggaa tagcttcgac aagcgtctga
    18481 taaatcacct ctccgcccgg cgcttcacca atgccggtat gcccggcgtt atcggtgagt
    18541 accacaatat tgcgggtgaa atatgcgtta tgtgccccac caatattaag caacatgcta
    18601 tcgtgcccgg cgaccggaat gactttcata tcagtaataa caggactgga ttgtgtcgtc
    18661 atcttattgc cccgcgacag gtttcagctc gatacgcttg atatcgccca ccagcaccag
    18721 gtagctcagt accgcgatta aggcatgaac accaacataa atcagcgccc cattaaacga
    18781 gccagtcgtg ccaacgatat aaccaattgc gattggcgtg acgatgccag aaatgttacc
    18841 gaacatgttg aacaggccac cggaaagacc gctgatctct tttggcgcgg tatctgccat
    18901 tactgcccaa cccagcgccc cgatgccctt accgaagaag gccagcgcca taaagccgat
    18961 gatcatccac tcaacgttga cgtagttgca gaacaccatc accatcgaca gcaacatgcc
    19021 cattacgatc ggtgttttac gcgcaatgtt cagcgacccc gtgcggcgca tcagccagtc
    19081 ggaaattatc ccacccagca caccgccgat aaaaccgcaa accgccggaa cggaagccac
    19141 aaagcccgct ttcagaatcg acatcccgcg cgcctgcacc agataaaccg ggaaccaggt
    19201 aatgaagaag taagtcaggg cgttgataca gtactgaccg atataaacgc cgatcatcat
    19261 ccgtgatccg agcagctgtt tgatctgccc ccacttcacg ctgaacggca ctttaacttt
    19321 ggtgttttgc tgatccatat tgatcagcgc gccacccgcg gcgatgtact ccagctcttt
    19381 ctgatttacc cccggatgtt gatttggctc gtggatgact ttcaaccaga taaagctgat
    19441 gacaatcccc agaccgccca taaagaagaa gacgtgtgac cagcccactt catgcgtcag
    19501 ccagcccata atcggcgcga agatcaccgt tgcgaagtat tgagcggagt taaaaatcga
    19561 caccgccgtt cccctttcct gcgccggaaa ccaggccgca acaatgcgac tgttgccggg
    19621 gaaagatggc gcttcagcaa gcccgaccag gaagcgcaga gtaaacaggg caacgataat
    19681 gccgaatcca ctaaagatat cgacgaagcc ttgcagcaag gtaaacatcg accagataaa
    19741 gatcgaccag aagtagacgc gttttgaacc aaaacgatcc agcaaccaac caccagggat
    19801 ctgcccgata acataagccc atgagaaggc agagaacaca tagcccattc ccacgggatc
    19861 aaggccgata tctttggcca tttccgaacc ggcaatagaa agcgtagcgc ggtcgccgta
    19921 gttgaaggat gtgacgataa acaacatcac cactatccag taacgagcat tcgtgcgttt
    19981 ttccacacgg ctcgcagcct gacttaaaga actcattgtt gcactcctga aaattcgcgt
    20041 tagccacgct cactctggac tgcgacatcg ccaggaaatc agaggtgacg tagggtgttt
    20101 tttgccgttt ttataggtcg ttcgccgaat acggcgcgtg tttatatctg gcaatagcag
    20161 tataaaaagc gcgccatagc ggctcaccgt gcaacaacac aacattaatg cgttcaatga
    20221 ggcccgattt tggcattagc cctggacggt ggaatccact tcacggaaat gaaaacaaga
    20281 acaagaaagg aagggttaaa acgaagaaat aaagaagagt atgaaatgga tcgcttgact
    20341 ccaggcaaac gccagtaaaa atccgcgcta tgaagcagtt tttactggca tttgcctgaa
    20401 aagattcgat tcagcaccgc taaaacgaca tttaccgctc gctgaacata tcaggacaac
    20461 agcgtgcccc actgttcgac ccacggattt gattccgttt ccggttccgg gttctcactg
    20521 gcgtcaatca acagcatttc gccaacccgc tgagcgctct gttcctgcaa caaggcatcg
    20581 aactgtttgc cgccattgca gaaatttaca taactgctgt cgccgagcgc aatcacgcca
    20641 taacgcagat tcggctggaa gcccagacta tctttgattc cctgaaagag cggcacaatg
    20701 ctatcaggaa ggtcgccctg cccggtcgtg gacgtaacca ccagaacata cttatcctga
    20761 tagggcagcc agtcgcttaa ttcaggatct tcaaataccg ttgctttgtg gccctgtgcg
    20821 gtcagaattg cttctgcttc ttccgccact aacagcgaat ttccgtacat ggtgccgaca
    20881 aaaataccaa tttccgccat accgttttct ccctggatta ggaacttatc tctccatcct
    20941 gacctgatgg cgcactgaac tcaacccttt cattttcagg aagcagaccg cgccagccaa
    21001 attgtgataa cgcctgcatc caggtgtcgt ccaggcccgc gtgaatagtc agcggctcgc
    21061 cagtaaaagg atgcgtcaat gacaactgac tggcatgcag cattaaccgc tgaagaccaa
    21121 aatgctcagc accgctgcga ttctggcgta aatcgccatg tttgctatcg ccaataatcg
    21181 gatgacgcaa atgggcaaga tgtcggcgaa gctgatgttt gcgtccggtt ttcggctcca
    21241 gttccaccag gccgtagcgc gtggtcgggt aacgtccggt cgctaccggc atttctacgg
    21301 tcgccagacc gcgataatgc gtcactgctg gctgcgggcc tttatcttcg cgggcaaatt
    21361 tatcagcgat tttgtccagt tcttccacca gtggataatc cagcaccgct tcttccatca
    21421 accagccgcg cacaatcgca tggtaacgtt tctggatttg gtgctgttca aactgttgtg
    21481 ccagcagccg tccggcctcg ctggataatc ccatcaacaa cacaccagaa gtgggtcggt
    21541 ccagacgatg agcagtaaaa acatgctgtc ctatctggtc acgcacggtt tgcatgacca
    21601 ctactttctc gtcgcgatcc agccagctgc ggtgaaccag ccagccggag ggtttattta
    21661 ccgcaaccag ccattcatcc tgatagagta tttccagcat tagctcgcat catccgcaaa
    21721 aagagcatcc agtttttcca actcagccag aataagcgcg cgttgcggat ggtccgtcgc
    21781 cagcgccatt tcataatagg gagcaacggc aaaagcgccc ggtaacggct ggttgttatt
    21841 taacaaatcg tgcattcgcg ggatcagcac ccactgcaac cactccagtg gttccatggt
    21901 gtccataaag aacggttggg tactattaaa ttgatgcggc tggggtacat cgtttcgcca
    21961 gtgctgatgt tcacgcagta acgcttcaag cgcctgcaac tggagacgaa cgcggtcatg
    22021 agtggtcata aagcaacctc aataagaaaa actgccgcga aggatagcag gaataaaaaa
    22081 agggagcact gtattcacag tgctcccggt tcgtttcgca gcattccagc tacatttgtt
    22141 gctccctgct catccttgac aacttttcct ctggccttgc ggccaatcgt tcatcctgaa
    22201 ctattgcttc ctgctcacac caccccgatg tgatacttca tcctgaagtg tccctggcct
    22261 tcctgaccca ccgaatcatc ctgaccggtt ctcattctcc atcctggagg tgtcctttaa
    22321 cgcgtcctgc gtcatcctct tcgcttcatc cagaagcctt tccctgaaac accatcctgg
    22381 tgtgtccttc agaagtgtca tcatcctgat gttcacttcg ttgtctgact ccctgtcgac
    22441 gaagatagaa tcgtcttttt cgcgaagtct tacaaggcgc ttacagacaa tgccttaagg
    22501 aaaatttcat atgaaagtta ggaattaaca agcaacatgt tgtttttaaa tgaaaataac
    22561 gattcttatc tttttaagta ttcgcttatt tcctggcgat ctcctacaga gtctgtaaga
    22621 gatctctcac aaccagatgg ggtatttgga ttacagaaga ggcttaagtt gattaaggaa
    22681 ttccgcaaga tttgaggcta aatgcgtccg tttacgtgtt cccagcgtct ctttacacac
    22741 ttcaccggag agattacaca ccgagatgac ctctaactct tcttccagcg tcgcgataaa
    22801 tagcgtgggt ggcagtttta gccttttctg agtcaccaga tgaccaatca gattttcctg
    22861 cacccgacgg aagtcgtctt cactccaggt ttgcagcaat gtcagtttga tatcgccaaa
    22921 ctgcgcgtgc atatccccgg caaactgagt cgtataaaag gtatgaattg tgggttgtat
    22981 cacaatatca aaagcgcgtt caaccgcgtt tacattttgt tccccggtaa acggctgggg
    23041 ttgccagtat acggcatctt cagtagttga aataatgcac ggtgaaggaa cgccgtacag
    23101 ttcctcactt aacggccagc ttttatgctc ttcatgccac gcatcgcagt agcgtgcagt
    23161 aaaatctttc agggcttgtg cggtcaaatc gtccactaat ttctctcttc acttaaacca
    23221 gatacacttg tcgtttagtt tatctggttt atgacggtga aacatgtctt cttatgcaaa
    23281 ccatcaggca cttgcgggcc tgactcttgg aaaatcaacc gattaccggg atacctatga
    23341 cgccagccta ctgcaaggcg ttccacgcag cctgaatcgc gatccgctgg gtctgaaagc
    23401 ggataacctg ccttttcaag gtgcggacat ctggacgctg tatgaacttt cctggctgaa
    23461 tgcgaaaggt ttgccgcagg tcgctgtcgg tcatgttgaa cttgattaca ccagcgtaaa
    23521 tctgattgag tcgaagagtt ttaagctcta tctcaacagt tttaaccaga cgcgttttaa
    23581 taactgggat gaggtgcgcc agacgctgga gcgcgactta agcacttgcg ctcagggtaa
    23641 tgttagcgtg gcgttatatc gtcttgatga actggaaggc cagccgatag gtcatttcaa
    23701 tggcacttgc attgatgacc aggatatcac tatcgataac tatgaattca ctactgacta
    23761 tctggagaat gccacctgtg gtgaaaaagt agtggaagag acgcttgtca gccacctgct
    23821 gaaatcaaac tgcctgatca cccatcaacc agattggggt tcgatccaaa ttcagtatcg
    23881 tggacgccaa attgacagag aaaaactgct gcgttacctg gtctcattcc gtcatcacaa
    23941 cgagttccac gaacagtgcg tggaacgcat ctttaatgac ctgttacgct tctgccagcc
    24001 agagaaattg agcgtttacg cacgttatac ccgtcgtggc ggtctggaca ttaacccgtg
    24061 gcgcagtaat agcgattttg tcccatcgac cacaagactg gttcggcaat aaattttttc
    24121 tcaattttgc gtgctggatt cacgcagaag gttgtgaaag gtcatcaggc agggctattg
    24181 taatcaaagg gaatgacgat attcgtccca taaggagttt ttcttgatta cacatattag
    24241 cccgcttggc tccatggata tgttgtcgca gctggaagtg gatatgctta aacacaccgc
    24301 cagcagcgac ctctatcaat tgtttcgcaa ctgttcactt gccgtactga actccggtag
    24361 tttgaccgat aacagcaaag aattgctgtc tcgttttgaa aatttcgata ttaacgtcct
    24421 gcgccgtgaa cgcggcgtaa agctggaact gattaatccc ccggaagagg cttttgtcga
    24481 tgggcgaatt attcgcgctt tgcaggccaa cttgttcgcg gttctgcgag acattctctt
    24541 cgtttacggg caaatccata acaccgttcg ttttcccaac ctgaatctcg acaactccgt
    24601 ccacatcact aacctggtct tttccatctt gcgtaacgct cgcgcgctgc atgtgggtga
    24661 agcgccaaat atggtggtct gctggggcgg tcactcaatt aacgaaaatg agtatttgta
    24721 tgcccgtcgc gtcggaaacc agctgggcct gcgtgagctg aatatctgca ccggctgtgg
    24781 tccgggagcg atggaagcgc cgatgaaagg tgctgcggtc ggacacgcgc agcagcgtta
    24841 caaagacagt cgttttattg gtatgacaga gccgtcgatt atcgccgctg aaccgcctaa
    24901 cccgctggtc aacgaattga tcatcatgcc ggatatcgaa aaacgtctgg aagcgtttgt
    24961 ccgtatcgct cacggtatca ttatcttccc tggcggtgtg ggtacggcag aagagttgct
    25021 gtatttgctg ggaattttaa tgaacccggc caacaaagat caggttttac cattgatcct
    25081 caccggcccg aaagagagcg ccgactactt ccgcgtactg gacgagtttg tcgtacatac
    25141 gctgggcgaa aacgcgcgcc gccattaccg catcatcatt gatgacgccg ctgaagtcgc
    25201 tcgtcagatg aaaaaatcga tgccgctggt gaaagaaaat cgccgcgaca ccggtgacgc
    25261 ttacagtttt aactggtcaa tgcgcattgc gccagatttg caaatgccgt ttgagccgtc
    25321 tcacgagaat atggctaatc tgaagcttta cccggatcaa cctgttgaag tgctggctgc
    25381 cgacctgcgc cgtgcgttct ccggtattgt ggcgggtaac gtaaaagaag tcggtattcg
    25441 cgccattgaa gagtttggtc cttacaaaat caacggcgat aaagagatta tgcgtcgtat
    25501 ggacgacctg ctacagggtt ttgttgccca gcatcgtatg aagttgccag gctcagccta
    25561 catcccttgc tacgaaatct gcacgtaatc tcccctcccc cggtgacgtt tgttgccggg
    25621 ttttctcgtt tttggacact tactcatcaa ctcatttcat ttgttatatg aatgtttctt
    25681 accaccctca cgcgacaaat atcatcacag ttaatatgtc atacaattta tattgcaacg
    25741 caaacgtttc cctattttca taaaccgtat tttatcgcta tgaaaaagaa attatcgcca
    25801 tgattaacta aaagtattga ttttttcagt tcaacctaca tatattgcgc gccccggaag
    25861 aagtcagatg tcgtttaatg ggcaaatatt gcccttaaat tctcttttac ttttgattta
    25921 cagagtaaag cgttgggata atctatcttc caagtagatt attgtatttg ggatcaagat
    25981 cactgataga tacataactt gtgtgtatct ttccgccctc aaattattac ggcggtaaat
    26041 gattaagcca tcgccgatag acagatttca tttttacggt caggcacctt cccgggctga
    26101 actggctaaa agctgaatta tttgcattcc tccaggagaa atagatggaa acgactcaaa
    26161 ccagcacgat tgcgtcgaaa gactctcgta gtgcctggcg caagacagac accatgtgga
    26221 tgctgggcct ttacggcacg gcaatcggcg cgggcgtgct gttcctgcca atcaacgccg
    26281 gtgttggcgg tatgatcccg ctgatcatca tggctatcct tgcgttcccg atgacgtttt
    26341 ttgctcaccg cggcctgact cgcttcgtac tgtctggtaa aaacccgggc gaagacatca
    26401 ccgaggttgt agaagaacac tttggtattg gcgcaggtaa actgattacc ctgctctact
    26461 tcttcgctat ttacccgatc ctgctggttt atagcgtggc aatcaccaat acagttgaaa
    26521 gcttcatgtc tcaccagctg ggtatgacgc caccgccgcg tgcgattctg tcgctgatcc
    26581 tgatcgtggg tatgatgacc atcgttcgct tcggtgagca gatgatcgtt aaagcgatga
    26641 gtattctggt gttcccgttt gttggcgtat tgatgctgct ggctctgtac ctgatcccgc
    26701 agtggaacgg cgcagcactg gaaacgctgt ctctggacac tgcatctgca accggaaacg
    26761 gtctgtggat gaccctgtgg ctggcaattc cggtaatggt gttctcgttc aaccactctc
    26821 cgatcatctc ttctttcgcc gttgcgaagc gtgaagagta cggcgatatg gcagaacaga
    26881 aatgctcgaa gatcctggca ttcgcacaca tcatgatggt gctgaccgta atgttcttcg
    26941 tcttcagctg tgtactgagc ctgactccgg cagacctggc tgcggctaaa gagcagaaca
    27001 tctcgattct gtcttacctg gctaaccact ttaacgcacc ggttatcgcg tggatggctc
    27061 cgattatcgc gattatcgct atcaccaaat ccttcctcgg ccactacctg ggcgcacgtg
    27121 aaggcttcaa cggtatggtg attaaatctc tgcgtggtaa aggtaagtct atcgaaatca
    27181 acaagctgaa ccgtatcact gcgctgttca tgctggtaac gacctggatt gttgccaccc
    27241 tgaacccgag catcctgggt atgattgaaa ccctgggcgg cccaatcatc gcgatgatcc
    27301 tgttcctgat gccgatgtac gcaattcaga aagtaccggc aatgcgtaag tacagcggtc
    27361 acatcagcaa cgtattcgtt gtcgtgatgg gtctgattgc aatctccgca atcttctact
    27421 ctctgttcag ctaagtcctt tcgcgccgct ttcgggcggc gcttcctccg ttttaacgcg
    27481 atgtatttcc tatgattagc gtattcgata ttttcaaaat cggcattggc ccttccagtt
    27541 ctcataccgt tggaccaatg aaagcgggta aacaatttac cgacgatctg attgcccgta
    27601 acctgcttaa agacgtgacc cgcgtggtgg ttgacgtgta cggctcgctc tctctgaccg
    27661 gtaaaggcca ccacactgat atcgccataa ttatgggtct ggcgggtaac ctgccggata
    27721 ccgtggatat cgattccatc cctggtttta ttcaggatgt gaatactcat ggtcgcctga
    27781 tgctggcaaa cggtcagcat gaagtggagt tcccggttga tcagtgcatg aacttccatg
    27841 ccgacaacct ttctctgcat gaaaacggta tgcgcattac cgcgctggcg ggtgataaag
    27901 tcgtttacag tcagacttac tactctattg gcggtggctt tatcgttgat gaagagcatt
    27961 ttggccagca ggatagcgca ccggttgaag ttccttatcc gtacagttca gcagccgatc
    28021 tgcaaaaaca ttgtcaggaa accgggctgt cactctctgg cctgatgatg aaaaacgagc
    28081 tggcgctgca cagcaaagaa gagctggaac agcacctggc gaacgtctgg gaagtcatgc
    28141 gcggcggtat tgagcgcggt atttccaccg aaggcgtgtt gcctggcaaa ctgcgcgttc
    28201 cacgccgtgc tgcggcacta cgccggatgc tggtcagcca ggataaaacc accactgacc
    28261 cgatggcggt tgttgactgg atcaacatgt ttgcactggc agtgaacgaa gagaacgccg
    28321 ctggcggtcg cgtggtgact gcgccgacta acggtgcgtg cgggattatc ccggctgtgc
    28381 tggcgtacta cgataagttt atccgcgaag tgaacgctaa ctcactggct cgttacctgc
    28441 tggtagccag cgccattggt tctctttata agatgaacgc gtcgatttct ggtgctgaag
    28501 tgggttgcca gggtgaagtt ggcgtggcgt gctcaatggc ggcggctggt ctggcagaac
    28561 tattaggcgc aagcccggcg caggtgtgca tcgcggcgga aatcgccatg gagcacaacc
    28621 tcggtctgac gtgtgacccg gtcgccggac aggtacaggt gccatgcatc gagcgtaacg
    28681 ccattgcggc agtaaaagcg gtgaacgccg cacgtatggc gctgcgccgt accagcgagc
    28741 cgcgcgtctg cctcgataaa gttatcgaaa ccatgtacga aacaggtaaa gatatgaacg
    28801 ccaagtaccg cgaaacctct cgcggcggcc tggcaatgaa gatcgttgcc tgcgattaat
    28861 cgctctccag aggcctcgtt ttgcgaggcc tcttcccgat ttctcatcca gccatagcct
    28921 gttccagtat cgaatgttac ccttatcgcc tgatctttaa gggggttatc gtggctgttc
    28981 atttgcttat tgtcgatgca ctgaatctta ttcgtcgcat tcatgccgtt cagggttcgc
    29041 cctgtgtcga aacctgccag cacgcgctcg atcagctcat tatgcacagc cagccaaccc
    29101 acgctgtcgc cgtttttgat gatgaaaacc gcagtagcgg ctggcgtcat cagcgtttac
    29161 cagattacaa agcgggtcga ccgccaatgc cggaagagtt gcacgacgag atgcttgcat
    29221 tacgcgccgc ctttgagcaa cgcggcgtcc cgtgctggtc aaccagcggc aacgaagccg
    29281 atgacttagc cgccacgctg gcggtcaaag tgacacaggc cgggcatcag gcaacgattg
    29341 tttcgacaga taaaggctac tgtcagttac tttcaccgac attacgtatt cgtgattact
    29401 tccagaaacg ttggctggat gcgccattta tcgataaaga atttggcgtt caaccgcagc
    29461 agttgcccga ttactgggga cttgcgggga tcagcagttc aaaggtaccg ggtgttgcgg
    29521 gaatcggacc aaaaagcgcc acacagctgc tggtcgagtt tcagagtctg gaagggatat
    29581 atgagaatct ggatgcggtt gccgaaaagt ggcgcaaaaa attagaaacc cataaagaga
    29641 tggcgtttct gtgccgcgat attgcccgct tacaaaccga tttgcatatc gatggcaatt
    29701 tacagcaatt gcgattggta cggtaacggc gagccggata cgccgcaaac gtcgtatccg
    29761 gcattatcac atcagcgcat ttaccaggcg gtatggtaaa gctctacaat atcctcaagc
    29821 gttgcttcac gcgggttgcc accggtacaa acatcatcca gtgccgcctg agccagtgcc
    29881 ggaatgtctt ccttgcgtac accaacatca cgcaaatgtg gcggaatacc gacatcacgg
    29941 ttgagagcaa acaccgcttc aacagcggca ttacgcgcct cttccagact cataccttcc
    30001 actttcacgc ccataacgcg cgcgatatcg cggtacttct caccggtaaa gtcagcgtta
    30061 tagcgcatga catgcggtaa caggatggcg ttcgcaacac cgtgtggagt gttgtaaaac
    30121 gcgcccagtg gatgcgccat accatgcacc aaccctaacc caacattcga gaagcccata
    30181 cccgcaacat actgcccgag cgccatttct tctccggcat ccttatcacc agcaaccgat
    30241 cctcgcagcg ccccagcaat gatttcaatc gctttaatgt gcagtgcatc ggttagcgcc
    30301 cacgcgccac gggtaatata cccctcaata gcatgagtga gcgcatcgac acccgtcgca
    30361 gctttcagcg ctggaggcat accatccatc atgtcagcgt caataaacgc cacctgcggg
    30421 atatcatgcg gatcaacgca aacaaacttg cgccgttttt cttcgtcagt gatcacgtag
    30481 ttaatggtca cttctgccgc agtgcctgct gtggtgggga ttgccagaat cggtacactg
    30541 ggtttattgg tcggggaaag tccttccagg ctacgcacat cggcaaactc cgggttgttg
    30601 cagataatgc caatcgcttt acaagtatcc tgtggagaac caccaccaat agcgatcagg
    30661 taatccgcgc cgctattctg gaatacaccg agcccttctt tgacgacagt aattgttggg
    30721 ttgggcacta cgccgtcgta aatcgcccat gccagccctg cagcatccat cttatcggtc
    30781 actttcgcca ccacgccgca ttgcaccagc gttttatcgg tgacgatcag cgccttctga
    30841 taaccacggc gtttcacctc atcggttaaa gccccaacag caccccgacc aaaccatgcc
    30901 gtttcgttca gaatcattct gttagccatc atccttctcc ttgttgcttt acgaaattac
    30961 tcttcaatcc gtaacccata ggttttgaat ttctccagca ctacggcaat ctcttcatcg
    31021 ctcagcactg gcaccgtgtc cgtaatcgcc agggtcgtca ggtaaagttg cgccagcact
    31081 tcaacttcat gcgccagcca taacgctttt tccagattca cctcacaagc gataagccca
    31141 tgatgttgta acaaagttgc cttacgattt ttgagagcca gcgcaacatg ttcagaaagt
    31201 tcgcgtgttc caaaggtcgc ataaggcgcg caaggaatag aattaccgcc agccgccgca
    31261 atcatgtagt gaatagcggg gatcggtcgg ttaagaatgg aaactgctgt gcaatgaacg
    31321 gcatgattgt gaacaaccgc gttggcatcc ggtctgcttt gataggccgc catatggaaa
    31381 cgccactcgc ttgaggggag ctttccttcc tcatgtttac cgttgccatc aataaaaaca
    31441 atatgcgact ccgtcagttt ttcatatgga atgcctgtag gcgtaatcag catcccatcc
    31501 tgataacgta cactgacgtt ccccgctgtc ccctggttca gtcccaggcg ggtcatttcc
    31561 aggcaagtgt caataatctg acgagcaagt ttatttcgtt ccattagcta cctctctctg
    31621 attcaaaaca gggcaataat gttgttcctt tcacactatt gaattagccg tttaattacc
    31681 caccatcttc ttcctgatta acaagaaaga aattcacaag cttatatttt gtgacctggc
    31741 tcaactaatc acagtaaata actgcaagtt ctctttttat aaccccatta aaaatgaccg
    31801 ctcttaaaaa tatttatcaa aacggtcatt tttctattca cctaagccag gaatgcccgt
    31861 tttcggcata aacaattaat acggtcatct ggtttgtgtt ttttatgatt tattttctga
    31921 aacgggcatg aaatttcgat tattaaagtg atggtagtca cataaagtca ccttccagct
    31981 aataagtgtg accgccgtca tattacagag cgttttttat ttgaaaatga atccatgagt
    32041 tcatttcaga caggcaaata ttcactgata tgaagcccga actcgctggt tttgcacttt
    32101 tgaaaacata accgattacg tgcttaagct tctgaaccta agaggatgct atgggaaaca
    32161 catcaataca aacgcagagt taccgtgcgg tagataaaga tgcagggcaa agcagaagtt
    32221 acattattcc attcgcgctg ctgtgctcac tgttttttct ttgggcggta gccaataacc
    32281 ttaacgacat tttattacct caattccagc aggcttttac gctgacaaat ttccaggcag
    32341 gcctgatcca atcggccttt tactttggtt atttcattat cccaattcct gctgggatat
    32401 tgatgaaaaa actcagttat aaagcaggga ttattaccgg gttattttta tatgccttgg
    32461 gtgctgcatt attctggccc gcggcagaaa taatgaacta caccttattt ttagttggcc
    32521 tatttattat tgcagccgga ttaggttgtc tggaaactgc cgcaaaccct tttgttacgg
    32581 tattagggcc ggaaagtagt ggtcacttcc gcttaaatct tgcgcaaaca tttaactcgt
    32641 ttggcgcaat tatcgcggtt gtctttgggc aaagtcttat tttgtctaac gtgccacatc
    32701 aatcgcaaga cgttctcgat aaaatgtctc cagagcaatt gagtgcgtat aaacacagcc
    32761 tggtattatc ggtacagaca ccttatatga tcatcgtggc tatcgtgtta ctggtcgccc
    32821 tgctgatcat gctgacgaaa ttcccggcat tgcagagtga taatcacagt gacgccaaac
    32881 aaggatcgtt ctccgcatcg ctttctcgcc tggcgcgtat tcgccactgg cgctgggcag
    32941 tattagcgca attctgctac gttggcgcac aaacggcctg ctggagctat ttgattcgct
    33001 acgctgtaga agaaattcca ggtatgactg caggctttgc cgctaactat ttaaccggaa
    33061 ccatggtgtg cttctttatt ggtcgtttca ccggtacctg gctcatcagt cgcttcgcac
    33121 cacacaaagt cctggccgcc tacgcattaa tcgctatggc actgtgcctg atctcagcct
    33181 tcgctggcgg tcatgtgggc ttaatagccc tgactttatg cagcgccttt atgtcgattc
    33241 agtacccaac aatcttctcg ctgggcatta agaatctcgg ccaggacacc aaatacggtt
    33301 cgtccttcat cgttatgacc atcattggcg gcggtattgt cactccggtc atgggttttg
    33361 tcagtgacgc ggcgggcaac atccccactg ctgaactgat ccccgcactc tgcttcgcgg
    33421 tcatctttat ctttgcccgt ttccgttctc aaacggcaac taactgaaca tattttccga
    33481 ataaagtgag gaatctgtaa tgaaaaaaat cagcttaccg aaaattggta tccgcccggt
    33541 tattgacggt cgtcgcatgg gtgttcgtga gtcgcttgaa gaacaaacaa tgaatatggc
    33601 gaaagctacg gccgcactgc tgaccgagaa actgcgccat gcctgcggag ctgccgtcga
    33661 gtgtgtcatt tccgatacct gtatcgcggg tatggctgaa gccgctgctt gcgaagaaaa
    33721 attcagcagt cagaatgtag gcctcaccat tacggtaacg ccttgctggt gctatggcag
    33781 tgaaaccatc gacatggatc caacccgccc gaaagccatt tggggcttta acggcactga
    33841 acgtcccggc gctgtttacc tggcagcggc tctggcagct cacagccaga aaggcatccc
    33901 ggcattctcc atttacggtc atgacgttca ggatgccgat gacacatcga ttcctgccga
    33961 tgttgaagaa aaactgctgc gctttgcccg cgccggtttg gccgtcgcca gcatgaaagg
    34021 taaaagctat ctgtcgctgg gcggcgtttc gatgggtatc gccggttcta ttgttgatca
    34081 caacttcttt gaatcctggc tgggaatgaa agtccaggcg gtggatatga ccgaactgcg
    34141 tcgccgtatc gatcagaaga tttacgacga agccgaactg gaaatggcac tggcctgggc
    34201 tgataaaaac ttccgctatg gcgaagatga aaataacaaa cagtatcaac gtaatgccga
    34261 gcaaagccgc gcagttctgc gcgaaagttt actgatggcg atgtgtatcc gcgacatgat
    34321 gcaaggcaac agcaaactgg ccgatattgg tcgcgtggaa gaatcacttg gctacaacgc
    34381 catcgctgcg ggcttccagg ggcaacgtca ctggaccgat caatatccca atggtgacac
    34441 cgccgaagcg atcctcaaca gttcatttga ctggaatggc gtgcgcgaac cctttgtcgt
    34501 ggcgaccgaa aacgacagtc ttaacggcgt ggcaatgcta atgggtcacc agctcaccgg
    34561 caccgctcag gtatttgccg atgtgcgtac ctactggtca ccagaagcaa ttgagcgtgt
    34621 aacggggcat aaactcgatg gactggcaga acacggcatc atccatttga tcaactcagg
    34681 ttctgctgcg ctggacggtt cctgtaaaca acgcgacagc gaaggtaacc cgacgatgaa
    34741 gccgcactgg gaaatctctc agcaagaggc tgacgcttgc ctcgccgcta ccgaatggtg
    34801 cccggcgatc cacgaatact tccgtggcgg cggttactct tcccgcttcc ttaccgaagg
    34861 cggcgtcccg ttcaccatga ctcgtgtcaa catcatcaaa ggcctgggac cggtactgca
    34921 aatcgcggaa ggctggagcg tggaattgcc gaaggatgtg catgacatcc tcaacaaacg
    34981 caccaactca acctggccaa ccacctggtt tgcaccgcgc ctcaccggta aagggccgtt
    35041 tacggatgtg tactcggtaa tggcgaactg gggcgctaac catggggttc tgaccatcgg
    35101 ccacgttggc gcagacttta tcactctcgc ctccatgctg cgtatcccgg tatgtatgca
    35161 caacgttgaa gagaccaaag tgtatcgtcc ttctgcctgg gctgcgcacg gcatggatat
    35221 tgaaggccag gattaccgcg cttgccagaa ctacggtccg ttgtacaagc gttaatacat
    35281 tactccctga tgtgatgccc ggtcgctccg gctaccgggc ctgaacaagc aagagtggtt
    35341 agccggataa gcaatgttat ccggctatat tgcaggagcg attatgaaac aagaagttat
    35401 cctggtactc gactgtggcg cgaccaatgt cagggccatc gcggttaatc ggcagggaaa
    35461 aattgttgcc cgcgcctcaa cgcctaatgc cagcgatatc gcgatggaaa acaacacctg
    35521 gcaccagtgg tctttagacg ccattttgca acgctttgct gattgctgtc ggcaaatcaa
    35581 tagtgaactg actgaatgcc acatccgcgg tatcgccgtc accacctttg gtgtggatgg
    35641 cgctctggta gataagcaag gcaatctgct ctatccgatc attagctgga aatgtccgcg
    35701 aacagcagcg gttatggaca atattgaacg gttaatctcc gcacagcggt tgcaggctat
    35761 ttctggcgtc ggagccttta gtttcaatac gttatataag ttggtgtggt tgaaagaaaa
    35821 tcatccacaa ctgctggaac gcgcgcacgc ctggctcttt atttcgtcgc tgattaacca
    35881 ccgtttaacc ggcgaattca ctactgatat cacgatggcc ggaaccagcc agatgctgga
    35941 tatccagcaa cgcgatttca gtccacaaat tttacaagcc accggtattc cacgccgact
    36001 cttccctcgt ctggtggaag cgggtgaaca gattggtacg ctacagaaca gcgccgcagc
    36061 aatgctcggc ttacccgttg gcataccggt gatttccgca ggtcacgata cccagttcgc
    36121 cctttttggc gctggtgctg aacaaaatga acccgtgctc tcttccggta catgggaaat
    36181 tttaatggtt cgcagcgccc aggttgatac ttcgctgtta agtcagtacg ccggttccac
    36241 ctgcgaactg gatagccagg cagggttgta taacccaggt atgcaatggc tggcatccgg
    36301 cgtgctggaa tgggtgagaa aactgttctg gacggctgaa acaccctggc aaatgttgat
    36361 tgaagaggct cgtctgatcg cgcctggcgc ggatggcgtg aaaatgcagt gtgatttatt
    36421 gtcgtgtcag aacgctggct ggcaaggagt gacgcttaat accacgcggg ggcatttcta
    36481 tcgcgcggcg ctggaagggt taactgcgca attacagcgc aatctacaga tgctggaaaa
    36541 aatcgggcac tttaaagcct ctgaattatt gttagtcggt ggaggaagtc gcaacacatt
    36601 gtggaatcag attaaagcca atatgcttga tattccggta aaagttctcg acgacgccga
    36661 aacgaccgtc gcaggagctg cgctgttcgg ttggtatggc ataggggaat ttaacagccc
    36721 ggaagaagcc cgcgcgcaga ttcattatca gtaccgttat ttctacccgc aaactgaacc
    36781 tgaatttata gaggaagtgt gaaatgctga aaacaatttc gccgttaatt tctcccgaac
    36841 tattgaaagt gctggcagag atgggacatg gagatgaaat tattttttcc gatgctcact
    36901 ttcccgccca ttcgatggga ccgcaggtga tccgcgctga tggcctgttg gtgagcgact
    36961 tgctccaggc gattatcccg ttatttgaac tggacagtta tgcaccgccg ctggtgatga
    37021 tggcagcggt agaaggtgac actctcgatc ctgaagtaga acgacgttac cgtaatgcgc
    37081 tttcactaca agccccgtgt cctgacatca tccgcatcaa tcgttttgcg ttttatgaac
    37141 gggcgcaaaa agcctttgcg atcgttatca caggcgaacg agcgaagtac gggaatattc
    37201 ttttaaaaaa aggggtaaca ccgtaatctc ataccggtac gcccgcatga cgcgggcggt
    37261 tatcgaatga tggggtgaaa aatatgaaag cggcacgcca gcaagcgata gtcgacctgc
    37321 tgctgaacca taccagcctg accacggaag ctctctctga acagctaaag gtcagtaaag
    37381 aaaccattcg tcgcgatctc aatgaattac agacgcaggg taaaattctg cgcaatcatg
    37441 gacgcgctaa atatatccac cgtcaaaatc aagacagtgg cgatcccttt cacatcaggc
    37501 tgaaaagcca ttatgcgcat aaagcagata tcgcgcgcga ggcgctcgcg tggattgaag
    37561 aagggatggt gatagcctta gacgccagtt caacctgctg gtatctggca cgccagttgc
    37621 ctgacatcaa cattcaggtc ttcaccaata gccatccgat ttgccatgaa ctcggtaaac
    37681 gcgaacgcat tcaactgatc agttccggcg gcacacttga gcgcaaatat ggctgttacg
    37741 tcaatccctc gctgatttcc caacttaaat cgctggaaat cgatctgttt attttttctt
    37801 gtgaagggat cgatagcagc ggcgcactgt gggactccaa tgccatcaac gctgattaca
    37861 aatcgatgct attaaaacgt gccgcgcaat cgttgttatt gattgataaa agtaaattta
    37921 atcgttcagg ggaagcccgc atcgggcatc tggatgaggt aacgcacatt atttctgatg
    37981 agcgccaggt tgcaacttct ttggtaacag cctgacggaa agggtagcag gccggagacg
    38041 acgccccggc cttgcctgtt atcgctcgtc gcgacgacca cccaccgcag cccagatgcg
    38101 gcggacgtgc accgtcactt cttcgcgatc gtgatacaac tgccgcgcct gaatctgagc
    38161 atttatgcca tgttcatcaa gctgtgcctg aatatacgcc agattgtgtg acacttcttc
    38221 gtagcgtttt ttcatcggca gtttgaggtt gaaaatggtt tcacggcacc agccattaac
    38281 cagccactgc gccatcaatg ccgcaacttt cgccggtttt tcaaccatat cgcataccat
    38341 ccaggagata ttgctgcgtg tcggacggaa tttgaacccg tcttcccgta gccacgtcac
    38401 ctgcccggta tccatcagac tttgcgccat cgggccgttg tcgacggaat aaacccacat
    38461 gttgcgcttc accagttggt aggtccagcc gcctgggcaa gcgcccaaat ccaccgccca
    38521 catcccgttc gccaggcgtt catcccactc atccgcagga ataaacacat gaaatgcctc
    38581 ttccagtttg agcgtggaac gactcggcgc atctgccgga aatttcaggc gcggaatgcc
    38641 catatagaac ggcgaattat tgttgctgta tgagtaaccg gtataacagc agcctggtgc
    38701 aatgaagaat acatgcacta ccggacgctt cggcgtttca tagttcgcca gcacccccgc
    38761 atcgcgcaag gcagcgcgta gcggcacggt gaatttacgg cagaatttca gcaactcttt
    38821 gctttcgttg gtatcggcaa cttcaacacg cagttcaccg cccttctcta ctacgccctg
    38881 taacatgccg acaatggggg taatacgatc ttctggcggc aaatgctgca ggagttcccc
    38941 caccacaaac cactggcggg caaaaattaa tgaactgaac ggcaactcac ggattaactt
    39001 atcgccatca tcaggttgat aacattcata aatgacataa cccgcattct ctttcacgcg
    39061 ggcaaaaccg aaaatttccc gctggccggc tttatcgcta atttctgcgg cgcactcttt
    39121 ttcaaagccc ggacggcaca gcaatacaac cttattcatg acttacgccc ttgcgcttta
    39181 aacggatagc accaactaac attaacgccc agcccgcaag gaagctcacg ccgccaaccg
    39241 gagtgacaaa cgcccacaaa cgcagatggg acagtgccag gcaataaagg ctgccgctga
    39301 acagcaccgt gcctaacgcg aggaaaacgc tactccagta aaaccagata ctgatgcgac
    39361 gctgcattgc caccgccaga cctaagatcg ccagcgtatg aaacgcctgg tattcgaggc
    39421 cggtttggat ccagcccatc tcaacggccc ccatggtttt acttaacaca tgcgcgccaa
    39481 aagcgcccag agccacaaaa atgaagccgc taatggcggc gaaaatcagc ataaaacggc
    39541 tggtcatggt cgtaccctac gtaaattatt gttcataacg aaagcggaat ttttcttgtt
    39601 cagcagcggc tttcgccagg atccattggc gaaaggcggc tattttaccc agttctgcct
    39661 ggctgtcatg acaaaccaga taaaaagcgt ttttactgac cagaacatca ttaaacgggc
    39721 aaacaagacg tccggcttca atttcagatt gcgccatcac gttatttgcc agcgccactc
    39781 cctgcccgtg aatagccgct tgcagcacca tggcgctatg gctaaaaatt ggcccttgct
    39841 gaacgttgat atgatttaac cccaattgac gagtatatgt ctgccagtca cggcgtgacg
    39901 catcatgtaa taacgtatgt ttagccagat cttccggggt cttcaagggt ttttcgccag
    39961 tcagcagtag cggcgaacac accggcaata aatattcggc gtacagtttt tccacccgta
    40021 gccccggcca gttgccccga ccataaaata tcgccacatc aacatcatcc gccagcttat
    40081 cttcctgacg atcaaccgcc tggattcgaa cgtcaattcc cggataagct gaattaaagc
    40141 tggaaagtcg cggaaccaac caatggatgg cgaaactggg gagtaaactg accgtcaacg
    40201 cccccttggc gctacgggcc tggagtttac gcgtcgcttc ggttaattgc gaaaatatct
    40261 ctttgatatc gaggaaatag ctttgacctt cctcggtcag caggagtgaa cgattacggc
    40321 ggcggaacag ttttagcccc agaaaatcct caagagactt gatttgatga cttactgcgg
    40381 cctgggtcac aaaaagctct tctgctgcgc gagtgaaact taaatggcgt gctgcggcat
    40441 caaaaactcg taaggcattt agcggtggta atcgtttaga catggctttt aaactttgat
    40501 gttaaatgaa tttaacaatt agatcacact gtgtaaccta ttagtttttt taatctgagc
    40561 cattataaat tgtccgttga gcttctacca gcaaatacct atagtggcgg cacttcctga
    40621 gccggaacga aaagttttat cggaatgcgt gttctggtga acttttggct tacggttgtg
    40681 atgttgtgtt gttgtgtttg caattggtct gcgattcaga ccatggtagc aaagctacct
    40741 tttttcactt cctgtacatt taccctgtct gtccatagtg attaatgtag caccgcctaa
    40801 ttgcggtgct tttttttacc ttgcgatcgc gaattactga tccagttcga ccatctcttt
    40861 cacgtcggta cggttgatct gctgtttgtt gccattagcg tctttatacg aaatcatacc
    40921 ggtatcgtta tcagtctgtg gtttgccgtc agagacgatg gtacgtccgt cattggtgtg
    40981 catcacatag ttcgaaccgg aacaggcgct cagggcaaaa gtcagcatac aggcagaaat
    41041 aattgcggca gtctttttca tcatcttctc cttaaagcga ttaataaatc ttaatagtat
    41101 tgattaacag gctaaaatta acgcctaaca ctattcagca tatgttactg acgcggcttc
    41161 gccaggatat ccagataatt ctgatggtta gcactctcct tgtatcaaag tgaattttgc
    41221 gtcacgatcg gtgcatcaag ccgaggagta ccatgaacgt ttttaatccc gcgcagtttc
    41281 gcgcccagtt tcccgcacta caggatgcgg gcgtctatct cgacagcgcc gcgaccgcgc
    41341 ttaaacctga agccgtggtt gaagccactc gacagtttta cagcctgagc gccggaaacg
    41401 tccatcgcag ccagtttgcc gaagcccaac gcctgaccgc gcgttatgaa gctgcacgag
    41461 agaaagtggc gcaattactg aatgcaccgg atgataaaac tatcgtctgg acgcgcggca
    41521 ccactgaatc catcaacatg gtggcacaat gctatgcgcg tccgcgtctg caaccgggcg
    41581 atgagattat tgtcagcgtg gcagaacacc acgctaacct cgtcccctgg ctgatggtcg
    41641 cccaacaaac tggagccaaa gtggtgaaat tgccgcttaa tgcgcagcga ctgccggatg
    41701 tcgatttgtt gccagaactg attactcccc gtagtcggat tctggcgttg ggtcagatgt
    41761 cgaacgttac tggcggttgc ccggatctgg cgcgagcgat tacctttgct cattcagccg
    41821 ggatggtggt gatggttgat ggtgctcagg gggcggtgca tttccccgcg gatgttcagc
    41881 aactggatat cgatttctat gctttctctg gtcacaaact gtatggcccg acgggtatcg
    41941 gcgtgctgta tggcaaatca gaactgctgg aagcgatgtc gccctggcta ggcggcggca
    42001 aaatggttca cgaagtgagt tttgacggct tcacgactca atctgcgccg tggaaactgg
    42061 aagcaggaac gccaaatgtg gctggtgtca taggattaag cgcggcgctg gaatggctgg
    42121 cagattacga tatcaaccag gccgaaagct ggagccgtag tttagcaaca ctggcggaag
    42181 atgcactggc gaaacgtcca ggcttccgtt cattccgctg tcaggattcc agcctgctgg
    42241 cctttgattt tgctggcgtt catcacagcg atatggtgac gctgctggcg gagtacggta
    42301 ttgccttgcg ggccgggcaa cattgcgctc agccgctact ggcagaatta ggcgtaaccg
    42361 gcacactgcg cgcctctttt gcgccatata atacaaagag tgatgtggat gcgctggtga
    42421 atgccgttga ccgcgcgctg gaattattgg tggattaatg acgaacccgc aattcgccgg
    42481 acatccgttc ggcacaaccg taaccgcaga aacgttacgc aagaccttcg caccgttgac
    42541 gcaatgggaa gaaaaatatc gccagttgat catgctgggg aaacagcttc cggctttgcc
    42601 agacgagtta aaagcgcagg ctaaagagat tgccggatgc gaaaaccgcg tctggctggg
    42661 atataccgtg gctgaacacg gtaaaatgca cttctttggc gacagcgaag gacgtatagt
    42721 gcgcggcctg ctggcggtgc tgttgactgc cgttgagggg aaaaccgccg ccgaattgca
    42781 ggcacagtcg ccgctggcat tgtttgatga gctgggattg cgtgcgcaac ttagcgcctc
    42841 acgcagccag gggctgaatg cgttaagcga ggcgattatc gctgcggcga agcaggttta
    42901 agcacagtac caggtcggat aagacgttcg cgtcgcatcc gacctgatta tttacccctg
    42961 acgagccgct ttcgccatca tctttttcag cgcatgagaa accgcaacaa aaccaaaggt
    43021 ggcggtcacc atcgtcgccg cgccaaatcc agatgcacaa tccatccgct tcggcccttc
    43081 tgccgtggct ttcatagcac acaccgtacc gtctgactgc gggtacacca atgcttcggt
    43141 agagaacacg caatcgacgc cgagcttacc tttactgttt ttcactacgc caaaatcgct
    43201 tttcaggcgc tcacgcaact tcgccgccag cgggtcctga atcgttttcg ccagatcggt
    43261 aacctgaatc tgcgtcgggt caatctgccc acccgcgcca ccggttgtga ccagtgggat
    43321 tttattgcgc cgacaataag caatcagcgc cgctttgggc cgtacactat caatggcatc
    43381 aatcacgtac gaataaccca cgctcatata ctgcgctacg ttatccggcg tcacgaaatc
    43441 atccaccacc gttacacggc actccgggtt aatctggcga atacgctccg ccataacttc
    43501 cgcttttgcc agcccgacgt tatcgcgcag agcgtgaatt tgccgattgg tattggtgac
    43561 gcacacatca tccatatcga taagcgtgat tgcgccaatc cccgtgcgcg ccagcgcttc
    43621 cgccgcccag gaaccgacac cgccaatacc gaccacacaa atatgcgcgt cagcaaacag
    43681 ttgcagcgct ttttcaccat acagacgcgc tgtgccacca aaacgctgac gccatgcatc
    43741 actaattacc acagacatag cacctcagaa taaaaagggc gaggttatcc ctcaccctaa
    43801 acgcataaat gcctgatgcg ctacgcttat caggcctaca taactcctgc aatattttgg
    43861 atttgcatga tttgtaggcc ggataaggcg ttcacgccgc atccggcatg aacaacgcgc
    43921 acttagtcaa taatcaggat gtgaggttac ccctcaccct gtcatatccg taaaaacggc
    43981 atacagaata tcacatcagc cactaaagac gttacctgcg cccggagccg ttttcagcac
    44041 ccaaacccgt ccatagtggt tgtaccaacc tgcgcggtgt ccggcttccg gcccgatccc
    44101 ttgatagata tcgaagtgtt ggcctttgat tgcgccaccg acatccagcg ccaccatcag
    44161 acgcaattcg tactgaccgt taaatttgcc gttattatcc agcagcggca cttctgccag
    44221 caaggttgta cctggcggaa taatggaacg atcagaggca actgaagcgc gaccaaccag
    44281 cggcaccgca cttgcccctt tcaccggtgc aaaagattgc ggtttaaaga agacaaaaga
    44341 cgggttctgt tccagcagct cgcgaacctc ggcttcactg tgtgtttcgc cccagtgacg
    44401 aatcgcctgc atcgacatat cttctttttt cacttcgcca cggtcgatca gcaccttacc
    44461 aatgctgcga taggcatgac cgtttttccc tgcatagctg aaaaagttaa gcggactgcc
    44521 atcaccaaag tcgatatacc cactaccctg cacatccata atgaagttat ccatcaggga
    44581 gttactgtaa gcgagaatat atttatcact caatgccccc gcgtagatct ccgcacgaga
    44641 cggcagacga ccacgttttg gcggcatacg gtaaatagga tactggaact cgccctggcg
    44701 ggtatggcgc gcctgaatta ccggcgtgta atagcctgta aactgcacgt ttccatagtt
    44761 gtcggcacct tccatctgcc aggcatcaat gccgaactgg cgcatattgc gggtatcacc
    44821 gcctgcgcgc agccactctt gcaccgcgtt ataaacatta ctctggttgc catacagacg
    44881 cggtgacgaa ttacggatat ggttaatttg ctcggcaaaa tcaccggcgt taatcggcgc
    44941 gccaacggca tctggctggt tcaccagaga gaaaggctgg gtaaatttcc cgtctttata
    45001 ttgctgtccg cgatcggttg gtttggaaga gcaggcggca agcattgcca caaccgtgcc
    45061 cataagaagg tactttaccc aacgtccttt cattgttctc ttcttcaggt taaaaaataa
    45121 ggcgcaacga agataacaaa ccgccgcgtt taatgaaatg agcagcctct ccctgacgca
    45181 aattttgcac aaaaaatagg ctttagtgat ttgtttttgt tcaaaatcat gccaaatccg
    45241 tgatcggggt aaaaaaaagg ttgcatgaaa acgcgagcgg agtatagtgc gcatccacgg
    45301 acgcggggtg gagcagcctg gtagctcgtc gggctcataa cccgaaggtc gtcggttcaa
    45361 atccggcccc cgcaaccaat tattgaacac cctaacgggt gtttttttgt ttctggtctc
    45421 ccataaaaaa gcgccattca gcgccttttt atcatcccct tctcgccagc gtcgccccat
    45481 cggcaaaata cgctttaatc cccgcaagaa tagactccgc aacttcctgc tggaaagtcg
    45541 ccgttttcag tttacgctct tcctcaacgt tactgataaa cgccgtttcg accagaatgg
    45601 agggaatatc tggtgccttt agtacggcaa acccggcctg ttcaacttga tttttatgca
    45661 gcttgttgat tttaccgagc ttattcagca ctgctttacc aaacttcagg ctgtcggcaa
    45721 tggtcagcga ctgcaccata tcgaacatgg tgtggtcgac atagcggtca ccgcttttgc
    45781 tcacgccacc aatcaagtcc gaggcgttct gggtttgtgc cagatatttt gccgcagtac
    45841 tggttgcacc tttggttgag agcgcaaaca cagaggaacc gctcggctga cgactggtaa
    45901 aggcgtcggc atggatagag acaaacaggt cagcacgctg tttctgagct tttgctacgc
    45961 gcacttgcaa cggaatgaag atgtcttcat tgcgcgtcat gtacaccttc atattgccct
    46021 ctttctcgat cagagagcgc agacggcgag ctatttgcaa taccacgtct ttttcgcgcg
    46081 ttttgtattt ccccaccgca ccggagtctt cgccaccgtg gccagggtca agcataatga
    46141 caatcggacg atcgcgccct gctttacctg gttgtggacc actttgcgct ggtggcacct
    46201 gcttttcgag gtcgcctttg ttgtaatcct ccagcagcgc cagcagcggg tcctgcatat
    46261 cctgtgcatt ggccggatag aggtccatca ccagacgctc tttaaacccg gcgaccggcg
    46321 caagggcaaa cagctgcggt tttacgtttt gctttaattc aaaaaccatc cgtacggttt
    46381 gcgggtcaaa ttgcccgacg cgcgccgact tgatgaacgg gtcgtctgca cgaatttgtg
    46441 ccgccatccc cttgagtacc gagttcaggt ttacatcttc gatatccacc accacgcgtt
    46501 ccgggttact caacgcgaac tgcttatatt tcagctgacg atttgattct accgtcacgc
    46561 gggtgtagct ggacgcaggc cagacgcgca ccgccacgac ctggctgacc gcagccagac
    46621 tgacctgact tacgctcaat agccacatgg cacccgcgcc ttgcagtaaa cgacgacggc
    46681 tgattgcagt gttggatcct gacatgcctc tcccgagcaa aagaaatcta agctgtgtaa
    46741 caagtaaacg actaatttga ccggaaactt tagcgaaaga cgcataatct gtcatctaat
    46801 aaacggtaaa cattcttttt atattcacgg cattactgat aaaaaagtcg ctctcgcata
    46861 aaatttacac ttgcaccctg cgaaaaaaca gaataaaaat acaataattt cgaataatca
    46921 tgcaaagagg tgtgccgtgg taaaggaacg taaaaccgag ctggtcgagg gattccgcca
    46981 ttcggttccc tatatcaata cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga
    47041 agccattgag catgagaatt tctccagtat cgttaatgat atcgggttgt tgcacagcct
    47101 cggcatccgt ctggtggtgg tctatggcgc acgtccgcag atcgacgcaa atctggctgc
    47161 gcatcaccac gaaccgctgt atcacaagaa tatacgtgtg accgacgcca aaacactgga
    47221 actggtgaag caggctgcgg gaacattgca actggatatt actgctcgcc tgtcgatgag
    47281 tctcaataac acgccgctac agggcgcgca tatcaacgtc gtcagtggca attttattat
    47341 tgcccagccg ctgggcgtcg atgacggcgt ggattactgc catagcgggc gtatccggcg
    47401 gattgatgaa gacgcgatcc atcgtcaact ggacagcggt gcaatagtgc taatggggcc
    47461 ggtcgctgtt tcagtcactg gcgagagctt taacctgacc tcggaagaga ttgccactca
    47521 actggccatc aaactgaaag ctgagaagat gattggtttt tgctcttcac agggcgtcac
    47581 taatgacgac ggtgatattg tctcagaact tttccctaac gaagcgcaag cgcgggtaga
    47641 agcccaggaa gagaaaggcg attacaactc cggtacggtg cgctttttgc gtggcgcagt
    47701 gaaagcctgc cgcagcggcg tgcgtcgctg tcatttaatc agttatcagg aagatggcgc
    47761 gctgttgcaa gagttgttct cacgcgacgg tatcggtacg cagattgtga tggaaagcgc
    47821 cgagcagatt cgtcgcgcaa caatcaacga tatcggcggt attctggagt tgattcgccc
    47881 gctggagcag caaggtattc tggtacgccg ttctcgcgag cagctggaga tggaaatcga
    47941 taaattcacc attattcagc gcgataacac gactattgcc tgcgccgcgc tctatccgtt
    48001 cccggaagag aagattgggg aaatggcctg tgtggcagtt cacccggatt accgcagctc
    48061 atcacggggt gaagttctgc tggaacgcat tgccgctcag gcgaagcaga gcggcttaag
    48121 caaattgttt gtgctgacca cgcgcagtat tcactggttc caggaacgtg gatttacccc
    48181 ggtggatatt gatttattgc ccgagagcaa aaagcagttg tacaactacc agcgtaaatc
    48241 caaagtgttg atggcggatt tagggtaagc gatgaaaatc gccggatgcg gcaggcgtaa
    48301 cactcgtgcg tcgcatccgg caattacgtt tattcccgcg aactaaacaa cgccgccaga
    48361 ccactgcgcc gctcagtacg agtggcgatt gccgcactta atatacgctc atcggcatac
    48421 agcgacagac ggcgacgcgc gcgggtcacc gcggtgtata ccagctctcg cgttactacc
    48481 ggcgtgcgtt ggctcggtaa aatcaacgcc gcatggtcga actccgatcc ctgcgattta
    48541 tgtaccgtca tcgcccacgt agtttcgtgc tctggcaagc gactcggttg cacagactta
    48601 atattgccgt ccggcatcgc aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata
    48661 ccgatatcgc cattaaacaa cccaagcgcg ctgtcattac gggcaatcat caccggtcgg
    48721 ccttcgtacc aacgagagtg cggattacga tgaattttgc gcttctgttg cataaactgc
    48781 tcaattcgct cattcagtcc agccacgcca aatggccctt cccgcagggc gcacaaaagc
    48841 tggtactcat tgaacgcctg aatgattaaa tccggctcgg cacgcgcttg cagcagatcc
    48901 agataacgtc cgtaacccgc aagagcttcc tcaagcatcg caatataatc ttcgccgctc
    48961 tgtaaaagtc gtttttcgat atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt
    49021 ttatcaccac ggttgatcgc cgcagctaac tgaccaatgc cagaatcgct gccgaaacga
    49081 tagctttttt gcagcaggca gagactgtcg cgcaaagatg ccgcttctgt gccagttcct
    49141 gccggaacgt gagtccccgt caggcggctt agctgcctgg cacgctcggc ggtaaagccc
    49201 gcgttggcat aagcgcagat atcgcccagc acagccccag cctcaaccga ggccagttga
    49261 tcacgatcgc cgagaaagat cactcgcgca tgatctggca aggcgtcgat cagtctcgac
    49321 atcataggca gatcgatcat tgacgcttca tctaccacca gcacatcaag atgcagcggg
    49381 ttaccggcat gatgacgtaa acgctggcta cccggctgcg cgcccagcaa tcggtgcaaa
    49441 gtgctggcat cttccggaat gcgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa
    49501 gccttgccga gagattcggt taagcgcgcg gcagctttac ccgttggtgc agccagacgg
    49561 atacggcagc gttcgccgtc tgccatttga attaacgctg ccagcaactt cgctacggtg
    49621 gtcgttttac cggtgccagg gccgccggaa atcaccgaga tccgccgcgt cagcgccact
    49681 gccgccgcaa ctttttgcca gttaatttca tcgcttactg gaaaaagttt gtccagggtt
    49741 tgcgccagta aagcttcatc aacctcaatg gcatgattca cttcgttgaa aaagcgtgcc
    49801 actgtgcgct cgttacacca catgcgattc aaataaagac gatcaccaca gaggatcatc
    49861 ggcgtgggtt catctccccg gctgaccgct tgtgaagcca gcaagcattc ttcccaattt
    49921 tgtagctcac cgatttcact gacacaggtc gccaacagcg gatgcgacgc ctcattattt
    49981 tccagtcgtg aaagcggcaa acaaacgtgt ccctctccgg catcatgact taacagtgcc
    50041 gccgcgaggg tgacggcagg atgttcatct cccgccacgg tcagggcaaa ttgcacatcc
    50101 agcgggcgta gctgtttgtg ctccacagct tccagtaatt gcttctgcaa tttcattacg
    50161 cctcctccag ggtcataccg gcaaacatct catccatcag ggtaatcaac tcggcgttgg
    50221 ggcgagttgt gtaaatcccc tgttgcggat gttctttatc aacgccacgc aggaacagat
    50281 aaataacgcc gccaaagtgg cgctcatagt cgtaatcagc aatgcgatgg cgcagataac
    50341 gatgcagcgc cagggtataa agctgatatt gcagatcata gcggtgtgcc tgcattgccg
    50401 ctgccatagc ctgttgggtg taagccgaac tgtcttcacc caaccagttg gatttatagt
    50461 cgagcaggta ataacgccct tcgtggcgga acaccaggtc gataaagcct tttaacatgc
    50521 cacgtacctg catgaactcc agcggcgggc agcctgcgga tagcgggtca aactggcgga
    50581 ttaacgtatc aagctgactg gcgataagcg gttcactaat cggcagataa aactccatct
    50641 ccacctgttt attgcgggcg gaaagttgac tcaggcttac gccggtttca ttgagaggtg
    50701 cctggaggac agccgtgatc cactcggtca ataccggttc ccactgcgat tcaaagccgc
    50761 cgagttccag tttttcccgc acccagttcg ggtcaaccgg ctgggtaaaa tccaggtctt
    50821 caaacaaact gtgcaagaac gtccccggtg acgcaccgcg cgggaactga tgcggtgtta
    50881 acgtcggttc ttcaacgacg ctgaccacgc ctgcggcatc aacatccagc cgtggcatca
    50941 gatcctgagc gataccgtga ccacgctgct gcaagccgga gtagctggtg acacgccagt
    51001 tatcgccggg caatcgttgt aacgtcttcg cattcagctc tgctgtagaa acatcattaa
    51061 cctgccaggg ttggttatca ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata
    51121 acgcttcaat acaggtgcga agccctgccg catcttgcgg ttcccctttt tgcagcaaac
    51181 gcccgagcgc actttggtgg acgtcggtgt cacctttttt atcgccacga cggcgcacca
    51241 gcggtgcaac gccgagacta caatgccaaa ccgaacgcgt cagcgccaca taaagcaaac
    51301 gcagatcttc cgccagacgt tcggcctccg cgagatcgac actttctggc gcagcgttaa
    51361 gatccagaac agcctcaaac gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga
    51421 aattggtaat aaatggcaac cagacaagcg aatattccag cccttttgat ttatggatcg
    51481 tgacaatctg caccagatgt ttatcacttt cgaggcgcat ttgttggctg gaggcattac
    51541 tgtctggctc gaggatatgt tgcgataacc agcgtaccag cgcatgttca ctttccagct
    51601 gcgttccggc ttcttgtagc agttcgctga tatgcaagat atcggtaaga cgccgctcac
    51661 cgcctgccgt tgccagcaag ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca
    51721 tcggcataac gccacgtttg cgccagattt gccgataacc atcgaactct tcgaccaccg
    51781 cgtcccacgc atgttcgtca ttattcagcg tttcgatatc cagcgcgttc agccccatca
    51841 ttgacgttgc cagcgcacta cgcagggtgt tctcacgttc gggcgtcatc accgcctgca
    51901 acaaccaaag catttcctgc gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt
    51961 aaacggaagg gatttccagc aacgttaagg catcgcgcac ctgggcagcc tcctggcggc
    52021 tgcgcaccag cacactgatg tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca
    52081 gcaacgcttc gccccgctgt ccggcttgta gccagtcgcg gatttgcgca gcacatacct
    52141 gcgccatggt actttgatag tcgccaacgc cgcagctttc gccttccatc agccacattt
    52201 tcatcgcagg ctgcgtttca cctttaaata caaaacgtaa cgcctgattt ttcccggctg
    52261 atttcactgg aataaacggt atttcgcgaa acatgaacgc gtcatcagtc tggctgaaaa
    52321 gcttgttcac gctgttcacc attcctggtg cggaacgcca gttggtgtct aaagtgtagt
    52381 gggcgtgaac ttcgctacgc gccttcatat aagtgaagat atccgcaccc cggaatgcat
    52441 atatggcctg cttcgggtcg ccaattagca acaatgcggt ttccggctga tggtgccaga
    52501 tacggcgaaa aattcggtac tgctgggggt cggtatcctg aaattcatcg atcattgcca
    52561 ccgggaatcg cgtacggatc gccgctgcca acacctcacc gctttcgcta cgcagcgcgg
    52621 aatcgagccg acttaacatg tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg
    52681 ctactgtttc gcggatctca gccaatgcgc gggtgatcac cagatcgcgg atcgacaatg
    52741 gttctgcaag cagttgatcg atcgcctcaa acagtggatg tcgcggggtt tcccccccgg
    52801 ccttcgtgcg atcttctaag aaacgctggg agaatttttc cagcgactcc ggcaactgat
    52861 aactgtttgt ctcttcttct gcccaggcgc tgatcttgtc gatccattta gcctgattgc
    52921 tacggttaaa cttgcgtcga tcaataccag aagattcgat cagcgcatcc agttcaccca
    52981 ctgcgtcgcg ccactgctgt tttaccgtat caatacgcgc cacaatttgc gcgtgacggg
    53041 aagccagcgt ttcatcatcg ggcggcggtg ctttgataac cggcgcttcg ccttgcagat
    53101 aacgattaat atcgctcagc aacgcctgcg gccctttcca ggtttcaaag acgacctggg
    53161 ctatttcacg cggcagcggg tagcagtggc gacgccagaa atcggcgcag gcctggtagc
    53221 gtagccgaga ctcatcttca atcagctgct gctcaaacag catgccggat tcaaaggcat
    53281 tcaggttgag catgcgctgg caaaagccgt gaatagtaaa gactgccgct tcatccatct
    53341 gccgttcggc taacaacaac cactgcgcgg cttgcgcttt atcgtcgatc tcttccagca
    53401 gacgttcgta cagtggattg tcggtggttt cacgcagaca ggcgatgcgc aactcgtgga
    53461 tattgctacg gatacgaccg cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca
    53521 gcagttcttc aacggtcagc gggcggggaa aggcggcgga accgcctagt ccaagtaaca
    53581 ggcgcaaata gagcgccgca atcgtaaagg ttttgcctgt gccggcagag gcttcaatca
    53641 ggcgctcgcc ctgtaagggc aagcgcaaag gatctagtgt ctcggcgaca tcactcattc
    53701 ttttcactca tcaggggcat tgtttgctgc aacgcgctga cgttctccca cactttccag
    53761 ccttcaggat gcacatattc ggctttcccg ttctggctgc cggaaatctg cgacagaata
    53821 gccatgcctt gcggctcgac caccgcctga tggaagaaat cagcaagttt ttgcggcgtc
    53881 agcagtttta tctgggccac gattttatca cgcgaatcga agcgcatatt gccgcgatcg
    53941 aaatctttac ttaacttcga tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg
    54001 gtaattaccg cctgctggat ttgcgcaaac tcatctggct tcatcgctcg caattttgcc
    54061 tctgcggttg ggaaaaacgc cttgtaacgc tcccacaaga atgaaggctg tttatcattg
    54121 ctttgcaaaa ggaagcccat gccccactga cgccccacgc tcattggaaa cgcaaacacg
    54181 gcatagccca attgttcttc ggtacgcaac tgattgtaga accacggctg tacgatctgc
    54241 cccaacagag agctataggc tgagctggtg tattcatcgt agccagtcgg tacaaatacc
    54301 gctgccagtg cggagtcggt gctgttaccg gctttttcaa agatgacgga ttgtttttta
    54361 tcgaccacta catctttgtt tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc
    54421 acatcgcgtg ccagcgttgt tgcctgggcc tcggtcatgt tgccgataac cataaactct
    54481 ggtcgagccc ctgattttaa ggcgtcgcga taggccagca cctctttcaa cgtaatggag
    54541 ggcaaaattt tacgccgttc atctcgcgag aagtacggca cttgcgagag catctgcgcg
    54601 ggcataatcg cctgctcaaa cgctttaccc ttttctgcgg aatccatcat ctggttatac
    54661 caggacttcg cctgctcaag ctgatcttcc gtagcggtat agctaaagta tccctcgagc
    54721 aatgcctgga acagctgcgg cagacgctgg gtgtaaccat tagcattaac cataaggccg
    54781 ttgttagcgt tggtggaaaa acttatgcca ccaaccgacg cctggttgct taactgatca
    54841 agcgccagcc ctgcgagata atcattgagc gcaaacatca cctgattgcg ggcgctgtcc
    54901 atggctttcg gattacgcaa aatcaggctg acatcagctt tgggttcgct ggcaaaataa
    54961 cggcttggcg catacaccac gcgcagattc gactcatcaa caatcagctc tggatggtca
    55021 tatttcttct ctgacttaat cagcgagaaa tcgtcaggaa tataggggtt aagctccggt
    55081 aaggagagcg caatactggc agctttttgc tgccagtcag cgaaagtttg ttcgctgatt
    55141 ttatcgacct gatacggcgc atcgacaaaa taagccgttt tattgtgcgg ctctttcggg
    55201 ctgatatacc agatacgcgc attctgcggc gtcatcatcg ccagacgttc ttttactgct
    55261 ttagcatcgt accgatcggc aatattgact gcatccagcg tatgctcaac cggaacgcga
    55321 atcatggtat ctgccagcca ttcgacgtaa tccatatcac gggtgattga cggataacgg
    55381 aagtcgatat ccagcacatt cgccagttca tcgaagtatt gtttatcgat ccctttttca
    55441 cgtaacaaat tgaggtagct aaaaatagcc gccacaacct gatcgcgatt cgccagacct
    55501 ttatcggtta aagacgcaga gatcgctaat acgccgctgt tgccgttgac gataggatcg
    55561 gagttggcgc taatgccctc aactaacccc tgcttttgca gccagtcaga cagtgtacct
    55621 gggctgcgat taccaatcag ataggtaatc aattcatccg ttttactgcg gaacttcgct
    55681 gagttgttat cgatgcgaaa ctcaacgcgc agaactttac gcggcagcgc tgggacgtaa
    55741 tgaatgataa tgcccttttg cgcgtcggtg actaccggca cggtgatttc cggttttttg
    55801 ctctctttgt tcggcacgcg accaaaggtg tccgccgcca tttttgccaa ctccggcagc
    55861 ggtttattac tgtaaataac cgccttcatc aaattggcgg aatagtactt ctcgtggaaa
    55921 tctttcagcg cctgctgcac cggattacca ggtttgtcgc ttaaagtttc gaggttacca
    55981 ccagaaaact ttgaaccggg gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg
    56041 cgcatcccgt cacgcgtacg cgccatggtt aattcagcgt tcactgcatt acgctcacgt
    56101 tcggcgtatt tcttgtcgag caagggttct gcaatagcat cggccaggcg gtctaccgca
    56161 ccgggcaagg cgtcgttctc aacttccaga tagaaagccg tgcgatacgg tgccgtgctg
    56221 gcattgtgac taccgccgtg cattttgaga tattcggcca gactgtcagc ctgcgggtac
    56281 tttttcgacc ccatcagact catatgttca aggtaatgtg ccagcccctg gtacgcctcg
    56341 ggatcttcca gcgacccaac gggcaccacc agcgccgaga gcgatttaac tgcctgcgga
    56401 tcagaaacca gcaagaccac cataccgtta tccagacgta tagcctgata ctggcggtta
    56461 tctttatcac ttttacggat ggtttcctga atcggctgcc atcccgtttc tgcctgactt
    56521 aatggtgccc aaagggcaac taacaacaat aatgctttga accaggtgct gcggggcatt
    56581 cacggacctc ataatcaact ttattttctg gccagattca acaggttaac gcaaatcatc
    56641 tgccagaatt taatcttgtg ctgcacgagt cagcctatgt ttatataacc atcagtccgt
    56701 gactggtgcg catcataaag taagcggata gattgcgcaa tttttataca gcgctcatga
    56761 ctgattaaag cgaaacagcg gtaacaggaa acgttgcgac tgttcaacga ttgcctccat
    56821 tgtctctggt gttaattgcc gccagagcct ttgataccag atatcatcac cttcgccacg
    56881 caccatcatg ttgccttcgt aagcctgaag gaatttcgta cgggcttttt gcaacgtgga
    56941 atcgtcatcc agcatggcat cgttttgcgc gtcataacag gtttttagcc acgcgccgcc
    57001 actttcaggt aacaccagca atggcgcgga catcccttca cgatacccct caatcagttg
    57061 tgagaggtaa tgcaaagcct gttcggctgc aagcggcgga aaacgccact cgccgtcttt
    57121 gcgtagaaaa aggcgacttt caccattacc accgctggca cagtagacaa ggtgttccag
    57181 ccaaagttgc attccctgcg ccacacttaa taaagaggga cgccagcgca acaagccatc
    57241 cggctgcacc tgcggcaacc aaccagttat ctgcacaccg ttgcaagcga gatcaatttc
    57301 catactctgc cccggctggc gacaggcaat gactctgtcg gcaagctgct gcatctcctg
    57361 gcactgtgtt tcccagaaaa tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg
    57421 gaagcggcgg aacaagcgtt cggcatcatc ctgctcaacc agtgcattca ataactgctg
    57481 attgatttga taacggctaa gtccttccag aataaatggc tcggtgtcgg ggatttcgct
    57541 gtcttcagta cggaagttca cctgcaaacg catctggaag aacgcccgca ccggatgtgc
    57601 ccagaatcgt tgtagcgttt ccagcggcac ggtttccggt aaggtaaacg gcagcggctg
    57661 aacaaattca gaatgtgctt taccagcctg gctggccgca ggtagccatt cacgagcata
    57721 gctttgtcgt tcgcctggct gatagttttg tggatcaaac ggcattcggg tatggaggca
    57781 agtaagatgc gcttttaccc ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg
    57841 tagataatga ctttgcccga tgtagtcgat cagttcctgc accagtaccg acgggaaacg
    57901 ttcactgtta tcctgaatgg aacgcccgat atagctgata tagagttttt gctgcgcgga
    57961 aattaacgct tccaggaaca gatagcggtc gtcatcgcga cggctacggt cgccacgctt
    58021 cggtttctgg ctcatcaggt caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc
    58081 gttcattccc agcaggcaaa ccactttgaa cggaattgaa cgcattggca tcagagtaca
    58141 aatgttaacc ggcccggcga gaaaacgctg gctgatacgt tcttgatcca ggcgctgtgc
    58201 cagttcatca cgcaatagtg acagcggcac cgcatcgcca tactgcgcac ctaaaccttc
    58261 ggcgataatc gcctgccatt gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc
    58321 cggcaggaag aaggcgttga gcatatcgcg acaaaccggc aaccactctt ccagcggacg
    58381 ctcctgtgcc agcccgcgac gccagatgtt tagctgcatt agcagtgaag ccagatgccc
    58441 caccagttct gcaattaagc cgctcgattc atcataaggt agaaccgatt gccactcgcc
    58501 ctgcgcgctc tccatcgcgt agcccaacaa catacgcgtc aggccaaatc gccaggtgtg
    58561 ttgtccggtg gcggggagtt ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat
    58621 gccggattcg ttgacccact gacgtaaata acgcagccct tcttcggtga tgtcaaaccg
    58681 cgccgccagc accggcacat ccagtaatgc cagcacgtct tccgacacaa agcggctgtc
    58741 tggcaacgat aacaggctga taaacgcttc aagtacagga tgcgactgcc gcgcccgacg
    58801 gtcggaaatg gcgtaaggca ggtaacgatc cgcaggtgcg ctaccaaaca cagcctgaat
    58861 aaacggactg tagctgtcga tatcagccac catcacgatg atgtcgcgcg gagtaagtgt
    58921 cgggtcttct tccagcatcg ccagcaggcg atcgtgcaaa acttcaactt cacgctgcgg
    58981 gctatggcaa acgtggaagg tgatactgct atccagtgga tcaagcgggc gtttgttatc
    59041 gctacgggaa aactcttcga tgttcacacc agcaacggcg cggttttcca gttccagaat
    59101 gtcagactga atgttatgca gcaggttatc tggcgtgaca tcgacaaagg cgtccagctc
    59161 ctggctgctc tccaggtcag aaaggagata aatgtagtcg cgcccaagct taccccatga
    59221 agccagcagc gggttgccga catcctgttc accatcgctg ttaaagagct gcccggcatt
    59281 ttcgctatcg cgaaatagcg gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgggt
    59341 cagcagtttc gccagataag caggatcttt aatatcgccc cagtaataac ggcaggggtt
    59401 ggtaaacagg agatggattt caatatgttt acccagcgcc tggagcgcct ggagataaac
    59461 aggcggtaac gcggaaatac cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt
    59521 cgtcgcggac tccagcgttt cgataaagcg ctgatagaga ttcgcgcggt gccagcgcgg
    59581 ttgcccgagt tcatgggtat attccaccag agccttccac aacggcgctt gccaggcctg
    59641 tgcttctccc agcccttcaa ccaaatgtcc tgtttcccat tgtgccagcc actccgggcg
    59701 atagaccaga tactggtcaa acaggtccgc cgcttttgag gaaagctgga acagttttcg
    59761 cttgtcgcta tcgtcagtca gataatgccg caacagggta aagtcttcgc gctccagcaa
    59821 ttgcggcagc agagtcatca gtttccagct catgctctgt ttgttaaagg cgctctcttt
    59881 ggggatctcc ggtaacaccc ggacgaacat atcccagata aagctcgcag gcagcggaaa
    59941 atcaatgttt gccgcaatac caaacttttg cgacagggtc atttgcagcc actgtgccat
    60001 accggtactt tgcaccagaa tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc
    60061 gacaataaac tccatcaacg cttccagcac gtccagacga ttggaatggt agacccttaa
    60121 catagcggct cctgactact gacgattcgg gcaatgcagg cgggtcatct cgcctgctct
    60181 gcccccgggt gaaactagcg taacgctgat gctgacacat cccgcctgcg atgtctgcat
    60241 tcggttgacc tgccagttgg caggtggcga aatcgcgcgc agttgcgttt gctgccaacc
    60301 atgccgccag agctgttggt actggtttct gctggcaaga ctgttcatta atgttcgctg
    60361 gaaacccgat aacgcagtga caatcatcac catcaacacc atcgccaaca ttacctccgg
    60421 caggctaaag ccttgttgat tcttcaggga agctgacata acgccccctc tttcagcgga
    60481 caaaaatcgc tccagccgcg tggcgaaaaa acaatttttc catcgatgac ttcacctgct
    60541 cgccacaacg aaacgccttc ataaccggca atcaataagg cttcattatc tgccaataaa
    60601 cgcaaacaaa cccgggcatc ggttccagcg tactgcaagc actgaactgc tggctgcgtc
    60661 tgccaggagt acatttttcc ccacgccagc gccgactgaa cgatggcctg gcggcgcaat
    60721 gactgacttt ccatgctcac gcgagaggca aaactgcgat cctgttggct cattccttgt
    60781 aatagcaagc tacccaaaac tagcagcatc aggaccagag ccagtgacga aacacccttt
    60841 tcgcggttca caggttgaat cctgtcacgc tatagctggc attcaccaca gtttgcggtt
    60901 cagacttact ggcagcacgc atattaaccg tcaacaccgg cgagaagccg ctgacatcct
    60961 gacgtacgac ctgaaaagtg tcgataatga tggcatccgg attagtgact ttatcccagc
    61021 ccttaccttc acaggatgtc gcaccgcgta gcgtttccag cacatgctcc ttcagacgaa
    61081 atccaatctg gtcggactct tttaccggtt cgcgatccca gataccgtta ctgttcgcat
    61141 cccactgcac aatgacacag tcaccctgtc cgacaatttc cagcccttcg ccggtacaga
    61201 tgccatgaca ataacccgcc ctctggagat gcttcgcgac ggtaaatacc cgcagccaga
    61261 tttcatcttc cagtgccagc ttacgggtgc tcgttaaact ttcacgctgt aacgcaggca
    61321 gaaagcgtgc agcccccaac aacaatacgc tactgatcgc catagcaatc aacacttcca
    61381 gcagagaaaa accttgctct tttacaggca tccttctgtt tctccttgct gacaaagccg
    61441 gagtcttccc cacggcgaaa ccaccagcca ccactcgccc gttgagtttt tgaagcgaat
    61501 atgcccggcc catgcggtat tgcgcaggcc aaagaaagca agcgaaggtg tcaggtcgct
    61561 catttcgact tcgggccagc gtggcacaaa gaccaatggt gaactgccat gacaggtatt
    61621 cgccccagca acggaactca caaggcacca taacgtcccc tccctgataa aactgatact
    61681 gtggtcgcgg ttatgccagt tggcatcttc acgtaaatag agcaaatagt cccgcgcctg
    61741 gctggcggtt tgccaaagcc gttgcgactg ctgccagtat tgccagccat agagtccact
    61801 tgcgcttagc gtgaccaaaa tcagcatcgc gaccagcgtt tcaatcagcg tataaccacg
    61861 ttgtgttttc atgccggcag tatggagcga ggagaaaaaa agacgagggc cagtttctat
    61921 ttcttcggcg catcttccgg actatttacg ccgttgcagg acgttgcaaa atttcgggaa
    61981 ggcgtctcga agaatttaat ggagggtaaa aaaaaccgac gcacactggc gtcggctctg
    62041 gcaggatgtt tcgtaattag atagccaccg gcgctttaat acccggatgc ggatcgtagc
    62101 cttcaatctc aaagtcttcg aaacggtagt cgaagatgga ttcgggttta cgtttgataa
    62161 tcaacttcgg cagcggacgc ggttcgcggc ttaattgcag atgagtttga tccatatgat
    62221 tgctgtacag gtgcgtatcg ccaccggtcc agacaaaatc acccacttcc agatcgcact
    62281 gctgcgccat catatgcacc agtaacgcgt agctggcaat gttgaacggc aggccgagga
    62341 agacgtcaca ggagcgctga taaagctggc aagagagttt gccgtctgcc acatagaact
    62401 ggaagaatgc atggcacggt gccagcgcca ttttatccag ttcgcctacg ttccacgctg
    62461 aaacaataat acggcgggaa tccgggtcgt ttttcagctg gttcagtacc gtagtgatct
    62521 ggtcaatatg acgaccatcc ggcgttggcc aggcgcgcca ctgtttacca tacactggcc
    62581 cgaggtcgcc gttttcatcg gcccattcgt cccagatggt gacattgttt tcgtgtagat
    62641 aagcaatgtt agtgtcgccc tgcagaaacc acagcagttc atggatgatg gaacgcaggt
    62701 ggcaacgttt agttgtcacc agcgggaatc catcctgcag gttaaaacgc atctgatgac
    62761 caaaaatgga aagcgttccg gttccggtac ggtcgttttt ctgtgtgcct tcgtcgagca
    62821 ctttttgcat cagttctaaa tactgtttca tggttcctca ggaaacgtgt tgctgtgggc
    62881 tgcgacgata tgcccagacc atcatgatca cacccgcgac aatcatcggg atggaaagaa
    62941 tttgccccat gctgatgtac tgcacccagg caccggtaaa ctgcgcgtcg ggctggcgga
    63001 aaaactcaac aatgatgcga aacgcgccgt agccaatcag gaacaagcct gagacagctc
    63061 ccattggccg cggtttacga atatacaggt tgaggataat aaacagcacc acaccttcca
    63121 gcagcagttc gtaaagctgt gatgggtggc gcggcagcac accgtaagtg tcgaaaatgg
    63181 attgccactg cgggttggtt tgcagcagca aaatatcttc tgtacgggag cctgggaaca
    63241 gcatggcaaa cgggaagttc gggtcaacgc ggccccacaa ttcaccgtta ataaagttgc
    63301 ccagacgccc ggcgccaaga ccaaacggaa tgagcggtgc tataaaatca gatacctgga
    63361 agaaggaacg tttggtgcgg cgggcgaaga taatcatcac cacaattacg ccaatcaggc
    63421 cgccatggaa ggacatgccg ccgtcccaga cgcgaaacag atacagcgga tcagccataa
    63481 actgcgggaa gttgtagaac agaacatacc caatacgtcc gccaaggaaa acgccaagga
    63541 agcccgcata gagtaagttt tcaacttcat ttttggtcca gccgctgccc ggacgattcg
    63601 cccgacgtgt tgccagccac attgcaaaaa tgaaacccac cagatacatc aggccgtacc
    63661 agtgaagcgc cacgggtcct attgagaaaa tgaccggatc aaactccgga aaatgcagat
    63721 agctactggt catctgtcac cacaagttct tgttatttcg ctgaaagaga acagcgattg
    63781 aaatgcgcgc cgcaggtttc aggcgctcca aaggtgcgaa taatagcaca aggggacctg
    63841 gctggttgcc ggataccgtt aaaagatatg tatatgatcc gcgctataac cctccgcgaa
    63901 tcagcccgcc catgccgcga cgctccataa aggctgcaac ctgatggcga acttcggtcg
    63961 ccagttgcgc ttccagacta cgctgcgcaa gattttctgc ttcggcataa tcaatgcgcc
    64021 gcagcaggta ttttacccgc gctacagaac gtccgttcat agacagatgg cgatacccaa
    64081 gcccaatgag gattgccacg cacatgggat cgcccgccat ttcaccgcac aaacggagat
    64141 cgattccatg tatttccgct tcccgggcga tcatcgccag agctcgtaac attgcaggat
    64201 gaagactgtc ataaatgttc gccacccggg tattgttgcg atcaacggcc agaatgtatt
    64261 gagtcagatc gttggtgcca acagagatga aatcgacccg ctttgccaga tgcggcagca
    64321 taaataccat tgacggtact tccagcatga tgccgatacg tggtttggga atttcgtaac
    64381 cgatcatctc ctcgacttca cgtccggcac gttcaatcag gcggcgagct tcgtcaactt
    64441 catcgaggct ggtgaccatc ggcaacagaa tattcaggtt gcccgtagcg gcgttagcac
    64501 gcagcatcgc ccgcacctgg atcaagaaga tctccggctg atcgagcgta atgcgaatcc
    64561 cacgccaacc caggcatgga ttctcttcgc tgatcggcat gtaaggcagc tgcttatctg
    64621 ctccgacatc cagcgtacgc aaggtgacgg gtttatcatt gaacatttgc agcatcccct
    64681 gatactgcgc cacctgttct tcttccgacg ggaaaccact ttgcagcatg aatgggattt
    64741 cagtgcgata aagaccgatg ccatcaatac ggctgcccag tttttcttca tgttccgggc
    64801 ttaaaccagc attgagcatg acttttatac gctcaccgct ttttaactgc gcgggtaaat
    64861 tgacgtcatc ttccgccaga cggctaagct caatctcttc actaattagc cgctgatatt
    64921 cttgcagcag taccggctcc ggatcgacca gcaattcacc gcgatagcca tcaacgatca
    64981 gcgtcctacg atgcagcacc gaaggctgaa tatccgcgcc catcacggta gggatcccca
    65041 gcgcacgtac catgatcgca gcatgggagt tggctgcgcc atctcgcacg acaacaccga
    65101 ctaagcgatc ctggggcagc tcagcaagag ttgtcgctga cagttcatct gccaccagaa
    65161 tgaaacgttc cggccaggcg ttcggccctt gattagcgtc atcaagatga aacagcaatc
    65221 gctgacccag cgcacgtaaa tcgccagcgc gctctttgag atagttatcg cttagcgcgg
    65281 caaactgttc ggcaaatttt tcaatgaccg tttttaccgc ccactctgcc accgagcctt
    65341 tatcaacctc ggcaaacaat tcgcgacgca gccgggtgtc cgaaagcagg tgcgagtaaa
    65401 gatcgaaaat agccgccgtt tctttttgtg caccggcggc aaagcgtttg ctgtagcggc
    65461 gaaactcgtt tgccgcttct tccagcgccc cggtcagtcg ttcgcgttcc agagccggat
    65521 ccagcgttga tgcctgatac acctgttcca ttaaaggtaa cgtggcatcc tgccagcctt
    65581 cggcaatcgc cacaccaggt gctgccggta atgcgcggat tcgcgtctgg cgatattgcc
    65641 caaacaaggc agtcaactgc gactgagaaa gaatagctgc catctgggtg gcaagcgtca
    65701 ccaggaagga ttcttcactt tcgtcatact ggcgcaactc tcgttgctgt accaccagta
    65761 caccaagcaa ctggcgacgt tgaataattg gtacgcctaa aaacgcgcgg aagcgttctt
    65821 cttttacgga ggggatgtat ttgaagctgg ggtgcttttg cgcatctgca aggtttatcg
    65881 gttccgccag cctgccaacc aggccgacga tcccttcatc aaacgcgagc gttacagtgc
    65941 gaccgcgtgg ttttttcagc ccccgggtcg ccatcaggta gtaacaacgt cgatcatgat
    66001 cggccaggta gaccgaacag acctcggtat ccatcgcaag acagatgtcg gtaaccagaa
    66061 tatttaacgc ctcattcagg cgtggtgcgc tggctacctt ttcgactatt tcgcgcaggc
    66121 gagtgagcat aattggcgtg acttaacctc ttttacgtcg ataagcagat gcgttttgtg
    66181 gttttggcgt attttcctgc agtgacatca ccacactcgc gaactctttc attaccctac
    66241 ggtagacatc acgtttaaat gacaccacct gtctgaccgg ataccagtaa cttacccatc
    66301 gccagccgtc aaactctggt gtactgctgg tttgcatatt gatttctgca tcgccgctca
    66361 ccagctgcaa gagaaaccat ttttgttttt ggccgataca aaccggcttc gtgtcccaac
    66421 gcaccaaacg tttcggtaat ttgtagcgca accagttacg cgttgaagca aggattcgaa
    66481 cgtctttgcg gcttaatcct acttcttcaa acaattcacg gtacatcgcc tgctctgcgg
    66541 attctccggg gttaattccg ccttgcggaa attgccagga gtgctgacca aatcgccggg
    66601 cccacattac ctgcccctgg cgattacaaa tcacgatacc tacgtttggg cggtagccat
    66661 cgtcatcaat caccggacta cctcaatata aagctttata tacgaatgat tgtttcatac
    66721 tccaggaaga cggtaaacca ctctctgcag ggcattacac actaataaca attgaataac
    66781 tcacagttat gtgcagagtt ataaacagag gaaggggtgg atagccgttt ttattcacct
    66841 tttctgtgga tagagttgtg aagaactacg gaattactac gggaaaaccc ggagcattct
    66901 gaataagccc gatatagaca ttttaaaata ttcattatca gtcaatgcat tacatgtaat
    66961 ttaagtaatg aaaaagagta attcgtgacc caggtcacac ctctcattta cgggttggcg
    67021 aaagatcaac caatgccgta tttatccaca gaatgtgcca ctaagttaag cactgaacca
    67081 ccaaaaactg gagtttcgtc gcacgtcaag gctgtaaatg gaaacagtag tggaggtttt
    67141 tcacagttat cccagctttc tgtggataac atggtgtaag atcctgttta ttttcagtga
    67201 ccagatttgg aaaacccgtt gcagtgttgc gcaactcgat taccggcaac ctaaaaagca
    67261 atataaatca gagaattgaa caacgcatgt ggaaaaagtt acactgcgaa tattcggcac
    67321 ataattgctg tttgtttttt aaccaaggta tcatgacatg tcccaacctc gcccactgct
    67381 ctctcctccc gaaactgaag aacagttgtt agcgcaagca cagcaacttt ctggttatac
    67441 attgggagaa ctggcggcac ttgccgggct ggttacgcca gagaatttaa aacgcgataa
    67501 aggctggatt ggcgtgttac tggagatctg gctaggtgcc agcgcaggaa gtaaacctga
    67561 gcaagatttt gctgctctgg gcgtggaact taaaactatc cctgtggata gtcttggtcg
    67621 tccgctggaa acaacattcg tttgtgttgc cccgttaacg ggcaatagcg gggtgacctg
    67681 ggaaaccagc cacgtgcgcc acaagctcaa acgcgtgctg tggataccgg ttgaaggcga
    67741 gcgcagcatc ccgctggcgc agcgtcgcgt tggatcaccg ttgctgtgga gcccgaatga
    67801 agaggaagac cggcagctac gcgaagactg ggaagaatta atggatatga ttgttctcgg
    67861 tcaggttgag cggattaccg ctcgtcacgg ggaatattta cagatacgac cgaaagcagc
    67921 gaatgcgaaa gcgcttaccg aagccattgg tgcccggggc gaacggattc tgacgctgcc
    67981 acgcggcttt tatttgaaga aaaatttcac cagtgcgctg ctggcccgtc attttctgat
    68041 ccagtagcca tcgctttgac ctgccgcttt ccgggcatat aattaccgct tcattttttt
    68101 ggcagggctt tttagatgtt atttgcatgg ataaccgatc ctaacgcctg gcttgcgctc
    68161 ggtacgctga cgctgctgga gatcgttctt gggatcgaca atattatttt cctttctctg
    68221 gtggtggcaa agcttcccac agcacaacgt gctcatgcgc gccgtctggg gttggcggga
    68281 gccatggtta tgcgtctggc gctgctggca tcaatcgcct gggttacgcg cctgacaaat
    68341 ccgcttttta caatattcag tcaggaaatt tctgcccgtg acttgattct gcttctgggt
    68401 ggcttgttcc ttatctggaa agccagcaag gaaatccacg aatccattga aggtgaagaa
    68461 gaagggctga aaacacgcgt ttcatcattc ctcggcgcta tcgtgcagat tatgctgctg
    68521 gatattatct tcagcctcga ttcagtgatt accgctgtgg gtctgtcaga tcacctgttt
    68581 attatgatgg cagccgtggt gattgccgta ggcgtgatga tgttcgccgc gcgctcgatt
    68641 ggtgattttg tcgaacgcca tccttcggta aaaatgctgg ctctctcttt cctgattctg
    68701 gtgggcttta ccctgattct ggaaagtttc gacatccacg taccgaaagg ttacatctac
    68761 ttcgcgatgt tcttctctat tgcggttgaa agcctcaacc tgattcgcaa caaaaagaat
    68821 ccgctctgat actccgtacg ctctcctgcg ggagggcgta ctcactcctg ttaccctcac
    68881 agtttaagat tttcctgctt tcaagatata taacgtcggt ttataaacag actattatca
    68941 taggtgggcc aaatgaggat aaccggatga aaaaatgggc agtaataatt tctgcagtcg
    69001 gactggcgtt tgctgtttcc gggtgttcca gtgattacgt catggcgacc aaagatggcc
    69061 gtatgatttt gaccgatgga aaacctgaaa ttgatgatga taccgggctg gtgagttatc
    69121 acgatcagca aggtaacgcg atgcaaatta accgtgatga tgtttcgcaa ataattgaac
    69181 gttaacaaat aaggtcagca tccggctggc cttaagattt ttctctttcc cttttccctt
    69241 ccctctgcca tttttatatt ccttatgtcg tgattataaa aaggaaacgg ctatgcaata
    69301 tcaccgtata ccccacagtt cgctggaagt cagcacgctg gggcttggca cgatgacgtt
    69361 tggtgaacag aacagcgaag ccgacgccca cgcacaactc gactatgccg tcgctcaggg
    69421 cattaacctt atcgacgttg ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg
    69481 gttaaccgaa acctacgtcg gcaactggct ggcgaaacat ggcagccgcg aaaagttaat
    69541 tatcgcctcc aaagtgagcg gaccgtcgcg caataatgac aagggcatcc gcccggatca
    69601 ggcgctggat cggaagaata tccgcgaagc gctgcatgac agcctcaagc gtctgcagac
    69661 tgattacctc gatctttatc aggtgcactg gccgcagcgc ccaaccaact gcttcggcaa
    69721 actcggttat agctggacag attctgcgcc tgcggtttcg ctgctggata cgctggacgc
    69781 actggcagag taccaacgag cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc
    69841 atttggcgta atgcgctacc tgcatctggc ggacaaacac gatctgccgc gtattgtcac
    69901 cattcagaac ccttacagtc tgttaaaccg cagttttgaa gtaggtctgg cagaagtcag
    69961 ccagtatgaa ggggtcgaac tgctggccta ttcgtgcctg ggtttcggca cgctgaccgg
    70021 gaaatatctc aatggtgcaa aacccgctgg cgcacgtaat acgctcttta gtcggttcac
    70081 ccgctatagc ggtgagcaaa cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg
    70141 tcatggcctg gatcctgctc agatggcgct cgcttttgta cgtcgtcaac cgtttgttgc
    70201 cagcactctg ctgggcgcaa ccacgatgga gcagctgaaa actaacgtcg aaagtttgca
    70261 tctggagttg agcgaagacg tattagctga aattgaagcg gtgcatcagg tttatacgta
    70321 tcctgcacca taagattaac gccggttaaa accggcgtta aatattaatg acggcgctgc
    70381 cagatccaca gcgccgttat tgccagcgca aatagcgcac cgaagccaat gccaatgggc
    70441 acgaccggga tgcctaccat taccgccagc gagtaaatgc ccagcatcaa caacatggcg
    70501 ctattttcgc caaggttttg tactgcaatc gcattccccg ccccgacgct ttttttaccc
    70561 cgctcctgta gcaacgcatt gagcggaacg acaaaaaagc cccccagcac gccaatcagc
    70621 atcaacaagg cataggctgg cagcagctcg tgttgcaggg agaaaatcag taccaccacg
    70681 ccaatcaaaa tcccggctgg catacagcgt gacacggttt ccagcgtaac cagcttcgct
    70741 gccgcacctg cgccaaccac gataccaatc gctaccatcg cgttgagata ggtcggcgta
    70801 gcgttatcgg taatgcccag cgccaccggt acccacagca ccaacaggaa acgcagcgtg
    70861 acacccgctc cccagaataa actggtgccc accagcgaaa aacgcgtttc accattgcac
    70921 catagcgagg tgcaggcatt caggaaactg cgggtcatgt tgatgagatt ccaggactgc
    70981 cccggacgcg ccgccgccag tttgggaatg tagatattgg caacgaccgc accaccgtag
    71041 gccagtgcgc atgcggccag ggcgaccagg acatgccagt cagccagcac accaccggct
    71101 acggaaccga gcaaaatcgc cgctatggta gaagcttcca ttaaaccgtt agcttttact
    71161 aacttactac ccgtggttaa ttcgccgaga ataccgtatt tcgccggtga ataggctgca
    71221 gcaccaacac ccaccagcgt atagccgaga aacggattga taccaaagca gatactggct
    71281 gcgcccagca gcttcaggcc gttggcaaac atcatcaccc ggcctttggc gaagctatcc
    71341 gccacctgcc cgacaaacgg cgcaaaaaga atgtaagcac ctacaaacac catttgcagg
    71401 atgggctggc tccactccgg atagaactgc gctttcagta acgccagagt ggcaaacagt
    71461 agtgcattat cgccaaacgc agagagaaac tgcgccacga taaccgcttt catccccttc
    71521 gaccacaacg aagtattagt gtgcactgac tcactcatcg tgttgttccg cttcgtctac
    71581 ccagcttttc aacgtgacaa agtcaggttt gccgctgcca agtaatggca tctgtttcag
    71641 ataacgaata tcacgcggta cagcaagctc cggcacaccg tgctcgcggg catactgttg
    71701 caacttatcg cgcgtcagtt cgttatctgt ggtgaaaagc accagtgcct cgcctttgct
    71761 ggcatcgctc ttaattgcag tggcatggac tttatctggc gaaacaccaa gtgccagttg
    71821 ttccaccatt tccagcgaca ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcgacc
    71881 ctgaatctgc acaaagccct gctcgtcaaa acgcacgata tcgccagtgt cgtaccagcc
    71941 gcgctccatt tcgccgcgca cattctcggc ggtgggcact tccagtacac ctggcttctc
    72001 cacccgcaga tagccgttca ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc
    72061 gataccagga accgacaaca ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc
    72121 gggtttcgcc gccatcggta cgttgataga aacgacaggc gcgcactcgg ttacaccata
    72181 gccttcaagg atgcgcaggc caaatttatc ctgccaaagc tgtttggtac tttcttgtag
    72241 tttttctgcg cctgccacca catagcgtag acgatagaaa tcatacgggt tggcgaaacg
    72301 cgcgtagtga ccgaggaaag tggaggtacc gaacaacacg gtgcaactgc ggtcatacac
    72361 caactccggc acaatgcggt aatgcagcgg gcttggataa aggaacactt ctgcacctgt
    72421 gagcagtggc gtaaacaggc ctaccgtcag cccaaaggag tgaaacagcg gtaacgccga
    72481 cataaagcga tcgttggtgg tgaagtcggc aatcgtttta atctgctcga cattcgccag
    72541 aatgctttta tggctatgga cgacgccttt cggatggcct tcagaaccgg aggtaaaaag
    72601 gatcagcgcc tcttcttccg gctgctgttt aacctgtgcc agacgcggca tcagcaaatg
    72661 agcgaagatc catactttgt cggcagtggt gacatctgct tttaaatctt ccagatagac
    72721 ccagcgcacc tgagtaagtt gctccggcag atgccagagt ttgcctttat cgagaaactg
    72781 gcgggaagtg aagatggttt tgatttcagc cgccgtaatg gcgctggtca gcccttttac
    72841 cccagcggtg tagttcatca ttgcgggaat gcggcgacgg gcgatggccc caaaaatcac
    72901 tgccgcactg atgcctgcat tgggcagcat taagccgatg cgttcgcctt caacactgta
    72961 tttttcaagg atgcgtccaa caaacagcgt tttagtaagc aatttgcgat aggagtctgg
    73021 ggtaaagttg acgtcttcga cacatttctt cccggctccg aagcggtaca ttgcactcag
    73081 taaagattcg tacagcgttt cacgcgggcg caccgccata cgcgcttcca tcattatttg
    73141 atgcagcatt tcgccagcga ttttgcgacg gtcacgggca cgcggcgcat ccggcatctc
    73201 cacctgcgtt ggtggcaaaa tatgcagagt aatttgcggg aacaagcgac gtttaaccag
    73261 acctttcagg cggctgaagt gcgtaagttc cgccccttca atacgcacag gaataaccgt
    73321 tgcaccagac ttcgccgcga caaaacccgc gccatcgtag attttcatca gcgagcctgt
    73381 ggtggtgatg cgtccttcag ggaaaatcac cactggtcgg ccctgttcca ccagacgtac
    73441 cagatgttta atcgccatag gttgcgtcgg gtcgagagga acaaagtcga taaatgattt
    73501 cagccaacgc atataccact gttgacttat tgaggtgtaa acggcaaata ccggacgtac
    73561 gggtaaaaac aatgccagca gtatgccatc aataaaagag acgtgattag gcgtgattag
    73621 aacgcgctcg cccttcaatg cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg
    73681 gcacaaattt cgaaaaaagc taaaaagcat tccaactccc tttgctctga ttcagtaaaa
    73741 gcgaatggag ggagattaca cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa
    73801 aaaaacctgc gcatccgcgc aggttggtgc aagagacagg gtacgaagag cgtaccgaat
    73861 aatctcacca atcaatacct ctgggatctt gattgtggtc tgcacgacga ctcttcgcca
    73921 gcgagaaaac gcaaaggaat gaagggaaat gcaacgaggt gtgtaaattg tcggttactg
    73981 ttacagattg atgaccggca aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat
    74041 gtgctcaacc cgaagttgac ttcacctatc aatacctctg ggatcaccac tttagcaacc
    74101 tgaagccaaa cgccaccagc ggtcaatcgc aacagcgttt cgcaaagtgt aaccaaaggt
    74161 ttgcaatctc tttttttgtc ttacttattt gcgtttggct cacattccca cgatgaaaac
    74221 acgccacccc ttgaaccaac gggcgttttc cgtaacactg aaagaatgta agcgtttacc
    74281 cactaaggta ttttcatggc gaccataaag gatgtagccc gactggcagg cgtttcagtc
    74341 gccaccgttt cccgcgtcat taataattca cccaaagcca gcgaagcgtc ccggctggct
    74401 gtgcatagtg caatggagtc tcttagctat cacccgaacg ccaacgcccg tgcgctggcg
    74461 cagcagacca ctgaaacggt cggtctggtc gttggtgatg tttccgatcc gtttttcggt
    74521 gcaatggtga aagcggtcga acaggtggct tatcacaccg gtaatttctt attgattggc
    74581 aacggttacc acaacgaaca aaaagagcgt caggctattg agcaactgat ccgccatcgc
    74641 tgtgctgcgt tggtcgtcca tgccaaaatg atcccggatg ccgatttagc ctcattaatg
    74701 aaacaaatgc ccggtatggt gctgatcaac cgtatcctgc ctggctttga aaaccgttgt
    74761 attgctcttg acgatcgtta cggtgcctgg ctggcaacgc gtcatttaat tcagcaaggt
    74821 catacccgca ttggttatct gtgctctaac cactctattt ctgacgccga agatcgtctg
    74881 caagggtatt acgatgccct tgctgaaagt ggtattccgg ccaatgatcg gctggtgaca
    74941 tttggcgaac cagacgaaag cggcggcgaa caggcaatga ccgagctttt gggacgagga
    75001 agaaatttca ctgcggtagc ctgttataac gattcaatgg cggcgggtgc gatgggcgtt
    75061 ctcaatgata atggtattga tgtaccaggt gagatttcgt taattggctt tgatgatgtg
    75121 ctggtgtcac gctatgtgcg accgcgcctg accaccgtgc gttacccaat cgtgacgatg
    75181 gcgacccagg ctgccgaact ggctttggcg ctggcggata atcgccctct cccggaaatt
    75241 actaatgtat ttagcccgac gctggtacgt cgccattcag tgtcaactcc gtcgctggag
    75301 gcaagtcatc atgcaaccag cgactaaccg cagttaaagc aattccagcg ccagtaattc
    75361 ttcgatggtc tggcgacggc gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg
    75421 taacagcgga cggctgttgt agttggatga cattgatgcg ccatatgccc ctgtatcatg
    75481 cagtaccaga taatcacccg ccttcacttc cggcaaggcg cgggtttcga catttccccc
    75541 ttcctgctgg gtaaagacat cgcccgattc acataacggc ccggcgacga cggtttccac
    75601 cgttggcgcg tgttccagag aacgaccatc agctgccaga gcactgatat ggtggtaact
    75661 accgtacatt gctgggcgca tcagatcgtt gaacccggca tcaacaagca caaagtggcg
    75721 gctacccatt tgtttgacgc tccgcacctg agtaattaat acgccagact gcgctaccag
    75781 gaagcgaccc ggttcaattt ccagtttcac agggtggccc aaatggcggg caatttgctc
    75841 acgcgcggca ttccacagac cataataatg ttcggtatca accgcctctt caccctgttg
    75901 ataaggaaca gaaagcccac cgcccgcaga aatagcctgt aaatcctgac cgaattcgag
    75961 gacctgacgc accatagcac cacacacctg ttccagatgg gcataatcaa cgccagaacc
    76021 aatgtgcatg tgaatgccga ccagctgcag atgatgacgt tgtatcacgt ccagtgcggc
    76081 gggcagatcg gtgtaccaga taccgtgctt gctgttttcg ccaccggtat tggttttttg
    76141 gctatgtccg tgaccaaacc ccgggttaac gcgcagccat acccgatgcc caggcgaaac
    76201 ctggcccagt tggtcgagca tatcaacaga acccgcattc accggaattt gcaattcact
    76261 gacgcgttca agcgtcgcct gatcgataac atctgccgta aaaacaatat catcggggtg
    76321 cgtttgcgga ttgtaacccg ccgccaacgc acgctctatt tcgcctaacg agacggaatc
    76381 cactttcacg ccctgctcac gcattaagcg caaaatatga atattggaac aggctttctg
    76441 tgcaaagcgc accacatcaa actgtttcag cgctgcaatc tgccgacgaa taatttgcgc
    76501 atcgtagacc cacaccgggc agccaaattc agcgggcaaa cgcagcagat tttcggcggt
    76561 gagagcggta tcggtgctga acagtgaatg tggcatagca aactccagat aagtgctttt
    76621 ttatgattac gccacatcat aaaaagaata aaaaatatcg atttatgtcg agtctatgca
    76681 aaaatgatat ggattaccgg attgcgagag agcgctaatg gccgccgtta acttacgtca
    76741 tattgaaatt tttcatgcgg taatgaccgc cggaagcctg actgaggcag cacacctgct
    76801 acacacctca cagccaaccg tcagccgcga actggcgcgc tttgagaagg tgatcgggct
    76861 gaaattgttt gagcgcgtac gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt
    76921 tgaagaagtg caacgatcct ggtacggact ggatcgcatt gtcagcgccg cagaaagtct
    76981 gcgcgagttt cgccagggag aactgtctat tgcctgcctg ccggtctttt cgcaatcttt
    77041 tttaccgcag ctcctgcaac cctttctggc acgttatccc gatgtcagct taaatatcgt
    77101 gccccaggaa tcaccgctac ttgaagagtg gctctcggcc cagcgtcatg atttaggact
    77161 cactgagaca ctccatacgc ctgcgggaac agaacgtacc gaattactct ctttagatga
    77221 agtgtgtgtg ttacctccgg gtcatccact ggcggtaaaa aaggtattaa cgccggatga
    77281 ttttcagggt gagaactaca tcagcctttc ccgtactgac agctatcgcc agttgctgga
    77341 tcagctattt actgaacatc aggttaaacg acgcatgatc gtagaaaccc acagcgccgc
    77401 gtcagtgtgc gcaatggtac gggcgggggt aggtgtttcg gtggttaacc cgctcaccgc
    77461 actggattat gcggcaagcg gtttagtggt gcggcggttc agtattgcgg ttccgttcac
    77521 cgtcagcctg atccgccccc tgcatcgccc gtcatcagcg ctggtgcagg cgtttagtga
    77581 gcatttacaa gcgggtttac cgaaactggt cacttctctt gacgcgattt tgtcgtcagc
    77641 tacgacagca taaaagcgac agcatcctcg gcatggatcg ccgcggtatc aaacacaggc
    77701 aaaacactga gctcttctgg caccagtaaa ccaatttctg tgcagccaaa aatgacgccc
    77761 tgtgcgccct gttctgcaag gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg
    77821 gtaaattgcc ccagacacag ttcttcaaaa ataatctgat taattttcgc ccgttcatcc
    77881 gcttcaggaa taagacaatt gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc
    77941 tgttccatgg tgtaacgcgt acccagcagc gccacacgag tcattccggc cccggtaatt
    78001 gcacgtccgg tggcatccgc aatgtgtaag aaaggcagag tgcaacgtga ctcaatggca
    78061 tccgccactt tatgcatcgt attggtacat aacacaatac cttctgcgcc cgcccgctgt
    78121 aagccaagcg ccgcctcagc cagaatgtcc ccggttttat cccattcccc gcgacgctgg
    78181 cactcttcta tttcatgaaa atcgacgcta tgtagcagca cttgcgcaga gtgaagccca
    78241 ccaagccgct gtttaatgcc ttcatttatc agacgatagt aaggaatagt ggattcccag
    78301 ctcattcctc ccagcaaacc aattgttttc attcctctct cctgatgtgt gttaccgcaa
    78361 caaggctgaa taaggaacga gacaaacgcc tcaacggcca ggtgcccaat ctctattaac
    78421 gaaaaaaggg ccggatgtac agcacatccg gcccgtgaaa tcagacgccg atatttctca
    78481 acttctcgcc tgccatcagt ttgcgttcga tatgttccag cgtgacattt ttggtttccg
    78541 gaatgagcca gaaagtgatg cctacaaacg caatgttcag cgcagtgtag agccagaacg
    78601 taccggcagc gccaatgcta tcaagtagtg tcaggaaggt cgcgccgata atcatattcg
    78661 acacccagtt tgtcgtcgtt gagcaggtga tgccaaaatc gcggcatttc agcggctgaa
    78721 tttcagagca caggatccac accactggcg cggcgctcat cgcataaccg gcaatacaca
    78781 tcatcgtcat gccaacagag agccaggaca agccactgga agccgtaccg ttatcaaact
    78841 gcatcaggca atagcccagc accagagtgc ctaacgccat cacgctgaaa ccaattttca
    78901 gagccggttt acgccctgct ttatctaccg taaagaccgc aatgaaggtc gcgaacataa
    78961 aggtcagtcc cacgaccaga gtcgcaatca tctgttgttc tgtggtcgta aagcccgcca
    79021 ttttgaagat acgcggcgcg tagtacatga tgatgttcat accggtaaac tgctgcatcg
    79081 cctgcaacaa cataccgaga aacacagcac gacggacgtt acggttgatc ttaaacagtg
    79141 cccaaccgcc ctgttttaac ttcaggcttt cacgaatttc gttgagttct tctcgcgctt
    79201 tttccgacgt atcgcgcagc atacgcaaca cttcttccgc ctcaatatga cgcccctttt
    79261 ccgctagcca gcgcgggcta tttggcagga agaccaccag aataatcagc aggactgctg
    79321 gtaaagcaag aacccccaac attgcgcgcc agttaccgct ataactgaac gctgtatcgg
    79381 ataaaaacgc cagcacgatg ccgagtgtga ccatcaactg gtacatactg atcatcttac
    79441 cgcgaacgtt ttcacttgcc atttcagaaa gatacagagg agcggtgtaa gacgcgatcc
    79501 cgacagcaat gcccagcacc acacgagcgg cgattaacat ctctacgctg gtcgcaaaag
    79561 cggaccctat agaaccgagt acaaacagga tggcccccgc catcaggctg tatttacgcc
    79621 ccaggcggaa cgacagccaa ccattaaaca gcgcaccaat tgctgcgccg agcatcatgc
    79681 tactaaccac ccattcctgc aaacgactgg tcagcacaaa gtgatcggta atgaacggca
    79741 acgctccggc gattacgccg atatcaagac caaataacaa tcctgcgacc gcagcagcta
    79801 ccgaaacaaa catattcata cgccgcgtat cccgcaaaga acgtggcgtt aaagcagatt
    79861 ccgtattgat agtaaccatt ttttcctgcc agcagagagt aagacatagt gaaaaaatac
    79921 gtgaacaact cacacaggtg tcaggtcgga aacagcataa atatggatta aattgctacg
    79981 acatgtcgtt atgtgatgga tattccaatt ttcaaattaa gttgaattat tgagattatt
    80041 attaaccacc taattttaca gcagataaaa ttcataaagt tcattaattg ataattgata
    80101 tggattattt cataaccatg atatggatta tgatgatcta caggtataaa aaaccctgcc
    80161 atgcggcagg gtcataaaag taagagaaat gaattaacgc gccagccaac cgccatccac
    80221 ggcaatggta taaccattca cataatctga agcgctggag gcaaggaaca ctaccggccc
    80281 catcaggtca ctcggcagtc cccaacggcc agctggaatg cggtcgagaa tttccgcgct
    80341 acgttgttca tctgcccgta gttgttgagt attgttggtc gccatgtaac ccggagctat
    80401 cgcattaaca ttaatgttgt gtttagccca ttcgttcgcc atcaatcgcg tcacgcccat
    80461 aacaccgctt tttgatgcgg tataagaagg cacacggatc ccgccctgga aggagagcat
    80521 tgacgcgata ttgataatct tgccgccatt gccttgcgcg ataaagtgtt tcgccgctgc
    80581 ctgagacatg aagaatacgc tcttgatatt caggttcatg acatcgtccc agtctttttc
    80641 gctgaactcg agagcatctt cgcggcgaat caatccggcg ttattcacca ggatatcaat
    80701 atgaccaaac tcagctaccg cgcgatccag cagtgctgga ataccatcaa tctttcgcag
    80761 atcggcggtc aggcttaaaa aacgacgccc cagcgccgtg acctgcttga tggtttcagt
    80821 cggttcaacg atgttaatgc caacaatgtc acagcccgct tgcgccagcc ccaacgccat
    80881 cccctggccc agtccagtat cacaaccagt gacgaccgca actttacctt cgagagaaaa
    80941 tgcacttaaa atcataacaa taccttattc gtttatgccg acaactagcg caaatcttta
    81001 acggccacat ggtccatatc atcaaagacc tggttttcac cgaccatgcc ccagataaag
    81061 gtataagctt tggttccgac gccggaatgg atcgaccagc tcggggagat caccgcctgc
    81121 tcgttatgca tcacaatgtg acgcgtttct tgcggctgcc ccatcatgtg gaaaacgcag
    81181 gcgtcatcat ccatattgaa atagaaataa acttccatcc ggcgctcgtg ggtgtgacac
    81241 ggcatggtgt tccacaagtt acctggagcc agctccgtca gccccatact caattggcag
    81301 gtttccagca catccgggac aaaatatttg ttaatcgtgc gacggttact ggtgaggtta
    81361 tcccctaacg tgactggaga tacttcgtcc ggtgtgactt ttttggtggg atacgtcgta
    81421 tgcgcgggta cgcaattgta ataaaacttc gccggagtgg cggtatcatc actggcaaag
    81481 acaacttctt ttgcaccttt acccacatac agggcgtcgc ggtgaccgat ttcatagcat
    81541 tgaccatcga cagtaatcgt accggcaccg ccaatattga taacacctaa ctcgcgacgt
    81601 tcaaggaaat agcttacgcc gagttgttta ccaacttccc cgccaacgga aaccgtttta
    81661 gttaccggca taatgccgcc aacaataata cggtcaatgt ggctgtaaac catggtgtac
    81721 tcatcggtga caaatacctt ttcaaccaaa aattcattgc gcagcccctg ggtatccagt
    81781 gtttttgcgt gcgcactgtg gatgctctgt cttacgtcca catcaacctc cgataatcag
    81841 tgaagcgtga aaatagaaaa cgatagtgaa acactgtttc gttttctcta taaggggatg
    81901 atatgcccat gggatagggt tttcaattaa aaataaaaca atgtttcatt tttaagttaa
    81961 ggattaaaaa aagtgccgtg tcgatcacga atatgtcatg agaatggaaa aatcagggag
    82021 cccagaaaat gtatgtcgac ttgccagcca tcattattca acagattaat gacggcagat
    82081 atttatcggt aaggaggaaa tgcagagcta ctcgtcacgt tcaatggtca atgctacgcc
    82141 ctggcccccg ccgatacaaa gcgttgccag tcctttgcgg gcattacgtt tcaccatttc
    82201 atgaaccaga gaaaccagga ttcggcaacc ggaagcgcct atcgggtgac cgagtgcgat
    82261 cgcgccacca ttgacattga cccgacgctc atcccattca agcatcttgc caaccgaaag
    82321 cgcctgtgca gcaaacgctt cattagcctc gataagatcg acatcagcca actgccagcc
    82381 cacacgctcc aggcaacggc gggtcgcata caccggcgca attcccatca atgctggatc
    82441 tacaccaacg ctggcaaatg cgcggatgcg ggccagcacg ggtaaattca acgctcgtgc
    82501 tttggcttcg ctcatcatca ttaccgcagc tgcgccatcg tttatggatg atgcattacc
    82561 cgctgtcaca gaaccgagac tatcaaatga aggatttaaa cgggctaagc cttctgcact
    82621 ggcgtcagtg cgtggctgtt catcggtatc aataaccaac gtctgaccat tactttgggt
    82681 cattaccggg acgatctcat ctttaaatcg tccggcgtca atcgccgctc gcgctttttg
    82741 ttgcgagcta agtgcgtaag catcctgcaa ctgacggctg atgccatatt cgcgagccag
    82801 attttcggcg gtgacaccaa tatgataatc attgaaggca tcccacaacc catcatgcac
    82861 cagactgtca accaactggc tattgccaag ctgtgcaccg gtgcggctat cagtcagaac
    82921 atgtggtgcg cggctcatgt tttcctggcc accggcgatg acaatatcag cctcgccaca
    82981 ctgtatcgcc tgagtagcca gatgcagtgc cttaagcccg gaaccgcaaa cgtcattaat
    83041 cgtgattgca gaaacgctat taggaagacc acctttaata gccgattgcc ttgccggatt
    83101 ctgccctgcc cctgcagtca acacctgacc aagaattact tcatccaccg catatgcagg
    83161 aacgccggta cgttctatta acgctttcac gaccagacta ccaagttcca cggcggaatg
    83221 acccgctaac gcaccacgaa agcagccgat aggtgtccgt aacgccccga caatcacaac
    83281 gtctttcatc acaacctcgc accaattaac agtgcaatag taaatgattg ttaattacag
    83341 ttatcttaat tgtttaaaaa aagtgatttt tatcacaaag gaaatatgcc tgagcagcag
    83401 tcagagacat aactggcacg taaggtttgc aaccactaac ccaccaatag aggggtagat
    83461 agggcgttaa tctcccatac ttaacctggt ttatggtaaa ttgccctcta ttttgtttaa
    83521 tttgtagatg atacgttcag ataatgtcta atatttggtc aaaagaagaa actctgtgga
    83581 gtttcgcgct ctacggcaca gccgttggtg caggcacgct cttccttcct attcagttag
    83641 gttcggcagg ggcagtggtc ctgtttatta ctgctctggt cgcctggcct ttaacatatt
    83701 ggccacataa agccttatgc cagttcatcc tctcatcgaa aacatcagca ggtgaaggga
    83761 taacgggcgc agtaacacac tactatggca agaagattgg taatctgatt accacgctgt
    83821 acttcatcgc cttttttgtc gtcgtgttga tatatgcagt ggcaattacc aactcactta
    83881 cggaacagct ggcaaagcat atggttattg atcttcgcat ccgtatgttg gtgagtctgg
    83941 gtgttgtatt aattctgaat ctcatttttc tgatgggacg tcatgccact attcgggtaa
    84001 tgggattttt ggtattccca ttgattgcct atttcttatt tctttccatt taccttgtcg
    84061 gtagttggca acctgatcta ttaacaaccc aggtagagtt caatcagaat acccttcacc
    84121 agatatggat atcgattccc gtgatggttt tcgcctttag ccatacgccc attatttcta
    84181 cgtttgccat agacagacgt gaaaaatatg gcgaacacgc tatggataaa tgcaaaaaaa
    84241 ttatgaaagt cgcttatctc atcatctgca taagtgtact gttctttgtc tttagctgcc
    84301 tgctttctat tccaccttcg tatattgaag cggctaaaga agaaggggtc accattttat
    84361 cggcgctttc tatgctgccg aacgccccag catggttgtc aatttccggg attattgtcg
    84421 cagtagttgc gatgtcgaaa tcattcctgg gtacgtactt tggcgttatt gaaggtgcca
    84481 cagaggtcgt caaaacaaca ttacagcagg ttggtgtaaa gaaaagtcgt gcatttaacc
    84541 gcgcactatc aattatgttg gtatcgctga tcaccttcat tgtttgttgc attaacccga
    84601 acgcgatttc gatgatttat gcgatcagcg gcccactcat tgccatgata cttttcatca
    84661 tgcctacgct gtcaacgtat ctcatcccgg cgcttaaacc ctggcgttcc atcggaaatc
    84721 tgattacgct gatcgtgggt attctgtgcg tatcggtaat gttctttagc taatatccat
    84781 tgtgaatggt tcaaaattgc tacaattttg aaccattcca tttttcataa tgttcatttg
    84841 ttattattcc gccctatcac ttctctcatc atatcctaga attatcagtt agttaaacac
    84901 acaaagagat ctgaacatct taaataagat tatttaagat gcataactta gattcgcaag
    84961 atatcttctg gtcattcaaa aacaatttgc gatatagata ttctcatacc atgagatagt
    85021 tatctggacc actctcaacc atgcaagatt aaatcagtga aacataatca tatttaataa
    85081 cgagaaatgc atttttaaat gtcttaatca gaattatagc aaatacagat
//
LOCUS       DAZFKF010000008        82228 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFKF010000008 DAZFKF010000000
VERSION     DAZFKF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 82228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.008"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1155
                     /locus_tag="ACHKA1_000665"
     CDS             <1..1155
                     /locus_tag="ACHKA1_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO5678366.1"
                     /translation="MLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTL
                     IQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPL
                     LAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELW
                     PLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSS
                     KLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKV
                     LVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNR
                     YDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1336..2826
                     /gene="dgcJ"
                     /locus_tag="ACHKA1_000666"
     CDS             1336..2826
                     /gene="dgcJ"
                     /locus_tag="ACHKA1_000666"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO5678367.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2869..3372
                     /gene="yeaK"
                     /locus_tag="ACHKA1_000667"
     CDS             2869..3372
                     /gene="yeaK"
                     /locus_tag="ACHKA1_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO5678368.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3373..3477)
                     /gene="yoaI"
                     /locus_tag="ACHKA1_000668"
     CDS             complement(3373..3477)
                     /gene="yoaI"
                     /locus_tag="ACHKA1_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO5678369.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3647..4093
                     /gene="yeaL"
                     /locus_tag="ACHKA1_000669"
     CDS             3647..4093
                     /gene="yeaL"
                     /locus_tag="ACHKA1_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO5678370.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4050..4871)
                     /gene="nimR"
                     /locus_tag="ACHKA1_000670"
     CDS             complement(4050..4871)
                     /gene="nimR"
                     /locus_tag="ACHKA1_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO5678371.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4968..6149
                     /gene="nimT"
                     /locus_tag="ACHKA1_000671"
     CDS             4968..6149
                     /gene="nimT"
                     /locus_tag="ACHKA1_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO5678372.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMSFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6204..6551
                     /gene="yeaO"
                     /locus_tag="ACHKA1_000672"
     CDS             6204..6551
                     /gene="yeaO"
                     /locus_tag="ACHKA1_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO5678373.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6573..6827)
                     /gene="yoaF"
                     /locus_tag="ACHKA1_000673"
     CDS             complement(6573..6827)
                     /gene="yoaF"
                     /locus_tag="ACHKA1_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO5678374.1"
                     /translation="MKIISFVLPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTTQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7010..8035
                     /gene="dgcP"
                     /locus_tag="ACHKA1_000674"
     CDS             7010..8035
                     /gene="dgcP"
                     /locus_tag="ACHKA1_000674"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO5678375.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            8068..8166
                     /gene="yoaK"
                     /locus_tag="ACHKA1_000675"
     CDS             8068..8166
                     /gene="yoaK"
                     /locus_tag="ACHKA1_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO5678376.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8169..8243
                     /gene="yoaJ"
                     /locus_tag="ACHKA1_000676"
     CDS             8169..8243
                     /gene="yoaJ"
                     /locus_tag="ACHKA1_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO5678377.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8302..8550)
                     /gene="yeaQ"
                     /locus_tag="ACHKA1_000677"
     CDS             complement(8302..8550)
                     /gene="yeaQ"
                     /locus_tag="ACHKA1_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO5678378.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8698..8880)
                     /gene="yoaG"
                     /locus_tag="ACHKA1_000678"
     CDS             complement(8698..8880)
                     /gene="yoaG"
                     /locus_tag="ACHKA1_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO5678379.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8884..9243)
                     /gene="yeaR"
                     /locus_tag="ACHKA1_000679"
     CDS             complement(8884..9243)
                     /gene="yeaR"
                     /locus_tag="ACHKA1_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO5678380.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9416..10054)
                     /gene="leuE"
                     /locus_tag="ACHKA1_000680"
     CDS             complement(9416..10054)
                     /gene="leuE"
                     /locus_tag="ACHKA1_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO5678381.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10181..11104)
                     /gene="dmlR"
                     /locus_tag="ACHKA1_000681"
     CDS             complement(10181..11104)
                     /gene="dmlR"
                     /locus_tag="ACHKA1_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO5678382.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFATVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQLILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11207..12292
                     /gene="dmlA"
                     /locus_tag="ACHKA1_000682"
     CDS             11207..12292
                     /gene="dmlA"
                     /locus_tag="ACHKA1_000682"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO5678383.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            12543..14153
                     /gene="yeaV"
                     /locus_tag="ACHKA1_000683"
     CDS             12543..14153
                     /gene="yeaV"
                     /locus_tag="ACHKA1_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO5678384.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGALLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            14185..15309
                     /gene="yeaW"
                     /locus_tag="ACHKA1_000684"
     CDS             14185..15309
                     /gene="yeaW"
                     /locus_tag="ACHKA1_000684"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO5678385.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15365..16330
                     /gene="yeaX"
                     /locus_tag="ACHKA1_000685"
     CDS             15365..16330
                     /gene="yeaX"
                     /locus_tag="ACHKA1_000685"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO5678386.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPLDTTHYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKIGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16384..17499)
                     /gene="rnd"
                     /locus_tag="ACHKA1_000686"
     CDS             complement(16384..17499)
                     /gene="rnd"
                     /locus_tag="ACHKA1_000686"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO5678387.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17581..19266)
                     /gene="fadD"
                     /locus_tag="ACHKA1_000687"
     CDS             complement(17581..19266)
                     /gene="fadD"
                     /locus_tag="ACHKA1_000687"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO5678388.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19471..20052)
                     /gene="yeaY"
                     /locus_tag="ACHKA1_000688"
     CDS             complement(19471..20052)
                     /gene="yeaY"
                     /locus_tag="ACHKA1_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO5678389.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(20092..20787)
                     /gene="tsaB"
                     /locus_tag="ACHKA1_000689"
     CDS             complement(20092..20787)
                     /gene="tsaB"
                     /locus_tag="ACHKA1_000689"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO5678390.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLSDIDALAYGRGPGGFTGVRIGIGIAQGLALGAALPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGVWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(20845..22755)
                     /gene="yoaA"
                     /locus_tag="ACHKA1_000690"
     CDS             complement(20845..22755)
                     /gene="yoaA"
                     /locus_tag="ACHKA1_000690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO5678391.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     KLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            22887..23231
                     /gene="yoaB"
                     /locus_tag="ACHKA1_000691"
     CDS             22887..23231
                     /gene="yoaB"
                     /locus_tag="ACHKA1_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO5678392.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            23653..23952
                     /gene="yoaC"
                     /locus_tag="ACHKA1_000692"
     CDS             23653..23952
                     /gene="yoaC"
                     /locus_tag="ACHKA1_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO5678393.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(24072..24251)
                     /gene="yoaH"
                     /locus_tag="ACHKA1_000693"
     CDS             complement(24072..24251)
                     /gene="yoaH"
                     /locus_tag="ACHKA1_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO5678394.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            24325..25686
                     /gene="pabB"
                     /locus_tag="ACHKA1_000694"
     CDS             24325..25686
                     /gene="pabB"
                     /locus_tag="ACHKA1_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO5678395.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            25690..26268
                     /gene="nudL"
                     /locus_tag="ACHKA1_000695"
     CDS             25690..26268
                     /gene="nudL"
                     /locus_tag="ACHKA1_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO5678396.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            26452..27816
                     /gene="sdaA"
                     /locus_tag="ACHKA1_000696"
     CDS             26452..27816
                     /gene="sdaA"
                     /locus_tag="ACHKA1_000696"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO5678397.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            27947..29545
                     /gene="pdeD"
                     /locus_tag="ACHKA1_000697"
     CDS             27947..29545
                     /gene="pdeD"
                     /locus_tag="ACHKA1_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5678398.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(29549..31105)
                     /gene="yoaE"
                     /locus_tag="ACHKA1_000698"
     CDS             complement(29549..31105)
                     /gene="yoaE"
                     /locus_tag="ACHKA1_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO5678399.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(31093..31251)
                     /gene="yoaL"
                     /locus_tag="ACHKA1_000699"
     CDS             complement(31093..31251)
                     /gene="yoaL"
                     /locus_tag="ACHKA1_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO5678400.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            31568..32539
                     /gene="manX"
                     /locus_tag="ACHKA1_000700"
     CDS             31568..32539
                     /gene="manX"
                     /locus_tag="ACHKA1_000700"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO5678401.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            32602..33402
                     /gene="manY"
                     /locus_tag="ACHKA1_000701"
     CDS             32602..33402
                     /gene="manY"
                     /locus_tag="ACHKA1_000701"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO5678402.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            33415..34266
                     /gene="manZ"
                     /locus_tag="ACHKA1_000702"
     CDS             33415..34266
                     /gene="manZ"
                     /locus_tag="ACHKA1_000702"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO5678403.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            34321..34779
                     /gene="yobD"
                     /locus_tag="ACHKA1_000703"
     CDS             34321..34779
                     /gene="yobD"
                     /locus_tag="ACHKA1_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO5678404.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            35090..35212
                     /locus_tag="ACHKA1_000704"
     CDS             35090..35212
                     /locus_tag="ACHKA1_000704"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678405.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
     gene            35209..35775
                     /gene="mntP"
                     /locus_tag="ACHKA1_000705"
     CDS             35209..35775
                     /gene="mntP"
                     /locus_tag="ACHKA1_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO5678406.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(35772..36581)
                     /gene="rlmA"
                     /locus_tag="ACHKA1_000706"
     CDS             complement(35772..36581)
                     /gene="rlmA"
                     /locus_tag="ACHKA1_000706"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO5678407.1"
                     /translation="MSFSCPLCHQPLTREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(36747..36956)
                     /gene="cspE"
                     /locus_tag="ACHKA1_000707"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(36747..36956)
                     /gene="cspE"
                     /locus_tag="ACHKA1_000707"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO5678408.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(36969..37112)
                     /gene="yobF"
                     /locus_tag="ACHKA1_000708"
     CDS             complement(36969..37112)
                     /gene="yobF"
                     /locus_tag="ACHKA1_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO5678409.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(37783..38070)
                     /gene="yebO"
                     /locus_tag="ACHKA1_000709"
     CDS             complement(37783..38070)
                     /gene="yebO"
                     /locus_tag="ACHKA1_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO5678410.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
     gene            complement(38145..38288)
                     /gene="mgrB"
                     /locus_tag="ACHKA1_000710"
     CDS             complement(38145..38288)
                     /gene="mgrB"
                     /locus_tag="ACHKA1_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO5678411.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            38447..38686
                     /gene="yobH"
                     /locus_tag="ACHKA1_000711"
     CDS             38447..38686
                     /gene="yobH"
                     /locus_tag="ACHKA1_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO5678412.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(38830..39621)
                     /gene="kdgR"
                     /locus_tag="ACHKA1_000712"
     CDS             complement(38830..39621)
                     /gene="kdgR"
                     /locus_tag="ACHKA1_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO5678413.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            39798..41171
                     /gene="yebQ"
                     /locus_tag="ACHKA1_000713"
     CDS             39798..41171
                     /gene="yebQ"
                     /locus_tag="ACHKA1_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5678414.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMADLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQILTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMYALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(41221..42102)
                     /gene="htpX"
                     /locus_tag="ACHKA1_000714"
     CDS             complement(41221..42102)
                     /gene="htpX"
                     /locus_tag="ACHKA1_000714"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO5678415.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(42294..44342)
                     /gene="prc"
                     /locus_tag="ACHKA1_000715"
     CDS             complement(42294..44342)
                     /gene="prc"
                     /locus_tag="ACHKA1_000715"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO5678416.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(44362..45060)
                     /gene="proQ"
                     /locus_tag="ACHKA1_000716"
     CDS             complement(44362..45060)
                     /gene="proQ"
                     /locus_tag="ACHKA1_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO5678417.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(45157..45654)
                     /gene="msrC"
                     /locus_tag="ACHKA1_000717"
     CDS             complement(45157..45654)
                     /gene="msrC"
                     /locus_tag="ACHKA1_000717"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO5678418.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            45784..47067
                     /gene="yebS"
                     /locus_tag="ACHKA1_000718"
     CDS             45784..47067
                     /gene="yebS"
                     /locus_tag="ACHKA1_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO5678419.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            47036..49669
                     /gene="yebT"
                     /locus_tag="ACHKA1_000719"
     CDS             47036..49669
                     /gene="yebT"
                     /locus_tag="ACHKA1_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO5678420.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            49749..51188
                     /gene="rsmF"
                     /locus_tag="ACHKA1_000720"
     CDS             49749..51188
                     /gene="rsmF"
                     /locus_tag="ACHKA1_000720"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO5678421.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAAQIRQAAASVGLNWDGNLRPWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            51306..51542
                     /gene="yebV"
                     /locus_tag="ACHKA1_000721"
     CDS             51306..51542
                     /gene="yebV"
                     /locus_tag="ACHKA1_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO5678422.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            51647..51838
                     /gene="yebW"
                     /locus_tag="ACHKA1_000722"
     CDS             51647..51838
                     /gene="yebW"
                     /locus_tag="ACHKA1_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO5678423.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(51839..52495)
                     /gene="pphA"
                     /locus_tag="ACHKA1_000723"
     CDS             complement(51839..52495)
                     /gene="pphA"
                     /locus_tag="ACHKA1_000723"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO5678424.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(52891..53232)
                     /gene="yebY"
                     /locus_tag="ACHKA1_000724"
     CDS             complement(52891..53232)
                     /gene="yebY"
                     /locus_tag="ACHKA1_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO5678425.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(53245..54117)
                     /gene="copD"
                     /locus_tag="ACHKA1_000725"
     CDS             complement(53245..54117)
                     /gene="copD"
                     /locus_tag="ACHKA1_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO5678426.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(54121..54495)
                     /gene="yobA"
                     /locus_tag="ACHKA1_000726"
     CDS             complement(54121..54495)
                     /gene="yobA"
                     /locus_tag="ACHKA1_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO5678427.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWRVVSVDGHKTKGHYTFSVK"
     gene            54634..54864
                     /gene="holE"
                     /locus_tag="ACHKA1_000727"
     CDS             54634..54864
                     /gene="holE"
                     /locus_tag="ACHKA1_000727"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO5678428.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            54966..55622
                     /gene="yobB"
                     /locus_tag="ACHKA1_000728"
     CDS             54966..55622
                     /gene="yobB"
                     /locus_tag="ACHKA1_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO5678429.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            55646..56308
                     /gene="exoX"
                     /locus_tag="ACHKA1_000729"
     CDS             55646..56308
                     /gene="exoX"
                     /locus_tag="ACHKA1_000729"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO5678430.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(56305..58365)
                     /gene="ptrB"
                     /locus_tag="ACHKA1_000730"
     CDS             complement(56305..58365)
                     /gene="ptrB"
                     /locus_tag="ACHKA1_000730"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO5678431.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRNGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(58574..59233)
                     /gene="yebE"
                     /locus_tag="ACHKA1_000731"
     CDS             complement(58574..59233)
                     /gene="yebE"
                     /locus_tag="ACHKA1_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO5678432.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(59559..59915)
                     /gene="yebF"
                     /locus_tag="ACHKA1_000732"
     CDS             complement(59559..59915)
                     /gene="yebF"
                     /locus_tag="ACHKA1_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO5678433.1"
                     /translation="MKKRGAFLGLLLVSVCASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVVWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(59982..60272)
                     /gene="yebG"
                     /locus_tag="ACHKA1_000733"
     CDS             complement(59982..60272)
                     /gene="yebG"
                     /locus_tag="ACHKA1_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO5678434.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            60406..61584
                     /gene="purT"
                     /locus_tag="ACHKA1_000734"
     CDS             60406..61584
                     /gene="purT"
                     /locus_tag="ACHKA1_000734"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO5678435.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(61640..62281)
                     /gene="kdgA"
                     /locus_tag="ACHKA1_000735"
                     /gene_synonym="eda"
     CDS             complement(61640..62281)
                     /gene="kdgA"
                     /locus_tag="ACHKA1_000735"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO5678436.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(62318..64129)
                     /gene="edd"
                     /locus_tag="ACHKA1_000736"
     CDS             complement(62318..64129)
                     /gene="edd"
                     /locus_tag="ACHKA1_000736"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO5678437.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(64364..65839)
                     /gene="zwf"
                     /locus_tag="ACHKA1_000737"
     CDS             complement(64364..65839)
                     /gene="zwf"
                     /locus_tag="ACHKA1_000737"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO5678438.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            66177..67046
                     /gene="hexR"
                     /locus_tag="ACHKA1_000738"
                     /gene_synonym="yebK"
     CDS             66177..67046
                     /gene="hexR"
                     /locus_tag="ACHKA1_000738"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO5678439.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            67174..68616
                     /gene="pyk"
                     /locus_tag="ACHKA1_000739"
     CDS             67174..68616
                     /gene="pyk"
                     /locus_tag="ACHKA1_000739"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO5678440.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(68747..69718)
                     /gene="lpxM"
                     /locus_tag="ACHKA1_000740"
                     /gene_synonym="msbB"
     CDS             complement(68747..69718)
                     /gene="lpxM"
                     /locus_tag="ACHKA1_000740"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO5678441.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLMIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(69838..71160)
                     /gene="mepM"
                     /locus_tag="ACHKA1_000741"
     CDS             complement(69838..71160)
                     /gene="mepM"
                     /locus_tag="ACHKA1_000741"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO5678442.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(71176..72108)
                     /gene="znuA"
                     /locus_tag="ACHKA1_000742"
     CDS             complement(71176..72108)
                     /gene="znuA"
                     /locus_tag="ACHKA1_000742"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO5678443.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            72187..72942
                     /gene="znuC"
                     /locus_tag="ACHKA1_000743"
     CDS             72187..72942
                     /gene="znuC"
                     /locus_tag="ACHKA1_000743"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO5678444.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            72939..73724
                     /gene="znuB"
                     /locus_tag="ACHKA1_000744"
     CDS             72939..73724
                     /gene="znuB"
                     /locus_tag="ACHKA1_000744"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO5678445.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(73938..74948)
                     /gene="ruvB"
                     /locus_tag="ACHKA1_000745"
     CDS             complement(73938..74948)
                     /gene="ruvB"
                     /locus_tag="ACHKA1_000745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO5678446.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(74957..75568)
                     /gene="ruvA"
                     /locus_tag="ACHKA1_000746"
     CDS             complement(74957..75568)
                     /gene="ruvA"
                     /locus_tag="ACHKA1_000746"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO5678447.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(75707..75772)
                     /gene="yobI"
                     /locus_tag="ACHKA1_000747"
     CDS             complement(75707..75772)
                     /gene="yobI"
                     /locus_tag="ACHKA1_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFO5678448.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            75843..76445
                     /gene="yebB"
                     /locus_tag="ACHKA1_000748"
     CDS             75843..76445
                     /gene="yebB"
                     /locus_tag="ACHKA1_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO5678449.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(76447..76968)
                     /gene="ruvC"
                     /locus_tag="ACHKA1_000749"
     CDS             complement(76447..76968)
                     /gene="ruvC"
                     /locus_tag="ACHKA1_000749"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO5678450.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(77003..77743)
                     /gene="yebC"
                     /locus_tag="ACHKA1_000750"
     CDS             complement(77003..77743)
                     /gene="yebC"
                     /locus_tag="ACHKA1_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5678451.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(77772..78224)
                     /gene="nudB"
                     /locus_tag="ACHKA1_000751"
     CDS             complement(77772..78224)
                     /gene="nudB"
                     /locus_tag="ACHKA1_000751"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO5678452.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(78342..80114)
                     /gene="aspS"
                     /locus_tag="ACHKA1_000752"
     CDS             complement(78342..80114)
                     /gene="aspS"
                     /locus_tag="ACHKA1_000752"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO5678453.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            80424..80990
                     /gene="yecD"
                     /locus_tag="ACHKA1_000753"
     CDS             80424..80990
                     /gene="yecD"
                     /locus_tag="ACHKA1_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO5678454.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLHRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            80987..81082
                     /locus_tag="ACHKA1_000754"
                     /pseudo
     CDS             80987..81082
                     /locus_tag="ACHKA1_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            81308..81556
                     /locus_tag="ACHKA1_000755"
     CDS             81308..81556
                     /locus_tag="ACHKA1_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO5678455.1"
                     /translation="MRVEICIAKEKISKMPKGSVPALQQEMLRRVGKRYDDVDVIVKS
                     ASNDGLTILRAVDKESAQEFVQETLQEVWETADDWFIH"
BASE COUNT        20058 a        21028 c        20092 g        21050 t
ORIGIN      
        1 atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt aagccttgca
       61 tttttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa accaattaac
      121 ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat tttccgccaa
      181 ctcagtttta tttgtttaac ctcgctggcg ctcttttgtt atggaaaaga caacatcctt
      241 gacaacaata agaaaaaaac tggcattctg ttgctggcgc tgattccttt tttagttttt
      301 ccccttctgg cacacaatct gagcagttat aacgctgact attctttgta tgtcgtcgat
      361 tactgtccgg acaaccatac tgcgacctgg ggaatcaact atacaaaaat attggtttgt
      421 ctgtgggcat ttttactgtt ctttattatc atgcgtacac gattagccag cgaactatgg
      481 ccgttaatag cattattatg tctggcatcg ctatgctgca acttacttct actgactctg
      541 gatgagtata attataccat ctggtatatc agtcgcggga ttgaagtctc cagtaaactg
      601 tttgttgtgt cttttctgat ttataacatt tttcaggaac tgcaactctc cagcaaactg
      661 gcagttcatg atgtgctgac caatatttat aatcggcgct actttttcaa cagcgtagag
      721 tcattattgt cgcgacctgt tgttaaggac ttctgtgtca tgctggttga tattaatcag
      781 ttcaaacgca tcaatgccca atggggacat cgtgtgggtg ataaagtgct ggtttcaatt
      841 gtcgatatta tccagcaaag catccgcccc gatgatattt tagcgcgact ggagggtgag
      901 gtgtttggct tgctatttac cgaactcaat agtgcccagg caaaaatcat tgcggaacgt
      961 atgcgtaaaa atgtcgaact cctgaccggc tttagtaaca gatatgatgt tcctgaacaa
     1021 atgaccatca gtattggcac ggttttttca acgggtgaca cgcgtaatat ctcgcttgtc
     1081 atgacggaag cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa caaagtgatt
     1141 attcatcata tttaagtgca aaaatattca gagccatgct ttttgcgtgg cttttgcata
     1201 caatttatta ctattacccc taaatttccc ctccccagcg gcgcagagat gagtataatt
     1261 agcgcccctg tgccaggccg caatcgaact ttatctggtt ttctcgtttc actaaccgaa
     1321 ggagtgccat ttatcatgaa attgcaccat agaatgctcc ggcattttat cgccgcaagt
     1381 gtcattgtgc tgacatcttc cttccttatt tttgaacttg tcgccagcga cagagcaatg
     1441 agtgcctatc tgcgctatat cgtgcagaaa gcagactcct cctttcttta tgataagtat
     1501 cagaatcaga gtattgccgc gcatgtgatg cgcgctctcg ctgctgagca gtcggaagtg
     1561 tcgccagaac agcggcgcgc catctgcgag gcttttgagt ctgccaataa tacccatggc
     1621 ttaaacctga ctgcccataa atacccgggc ttacgcggca cactacaaac cgcatccact
     1681 gactgcgaca caattgtgga agctgcagca ctattacccg cttttgatca ggcagtggaa
     1741 ggcaaccgcc accaggatga ttacggttca ggtcttggga tggccgaaga gaaatttcac
     1801 tattatctcg atctcaatga ccgctatgtc tatttttatg agccggttaa tgttgaatac
     1861 tttgctatga ataactggtc cttcctgcag tcaggaagta ttggcatcga tcgcaaagat
     1921 attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca tttaccagga tcagcgtact
     1981 aaacagaatg tgatgagttt gctgacgccg gtgtatgttg cagggcagct aaaagggatt
     2041 gtgctgctgg atattaacaa aaacaatctg cggaatatct tttacactca tgaccgccct
     2101 ctcctctggc gttttctcaa tgtcacgcta accgatacag attcggggcg cgacattatc
     2161 atcaaccaga gcgaagataa tctgttccag tatgtcagtt acgtccatga cttaccgggc
     2221 ggcattcgtg tctcgttatc cattgatatt ctttacttta tcacgtcttc gtggaaaagc
     2281 gttctgttct ggattttgac ggcgttaatt ttgctgaata tggtgcgaat gcacttccgt
     2341 ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga tgactggact gtataatcgc
     2401 aaaattttaa ctcctgaact ggagcagcgg ttgcagaaac tggtgcaatc cggttcttcg
     2461 gtgatgttta ttgctattga catggacaag ttaaagcaaa taaatgacac cctcggtcat
     2521 caggaggggg atttagcgat tacgttatta gctcaggcga ttaaacaatc gattcgtaaa
     2581 agtgattatg ccatccgact cggtggcgat gaattctgca tcattcttgt cgattcgacg
     2641 ccgcaaattg cagcacaact gcctgaacgt atcgaaaaac gtctgcaaca tatcgcgccg
     2701 cagaaagaga tcggcttctc ttccggtatt tacgcgatga aagaaaacga tacgttacat
     2761 gatgcgtata aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt
     2821 tcatgataac cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg
     2881 gctaaaggaa gcgtgacgca tcagcggtta atcgctttat tatcacaaga aggtgcagac
     2941 ttccgcgtcg ttacccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggc
     3001 accgctctag gccagggtgc taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat
     3061 caacatgttc tggcgatcct cgcagccgat caacaggccg atctgagcca actcgccagc
     3121 catataggcg gattacgcgc ttcgttggcc agcccagcgg aagtcgatga actcacaggc
     3181 tgtgtcttcg gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac
     3241 cctctactct tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc
     3301 gttattttga aaaccgcgga ttatttgcgc attgcgcaac cagaactggt caatttccgc
     3361 cgcactgcgt agctagcctg tccgttcaat aagcagaacg gacaaaacca gcacaaccgc
     3421 aatggcaaaa aacgatgacg tgataatcag tgtctcgacg aacatttgat cgttcatagc
     3481 atgcccccgg gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat
     3541 gacagttcgc ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc
     3601 aagcgtaaag taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac
     3661 tttgctgatc ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc
     3721 cgtttcaatt ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg
     3781 gattgaaaaa caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc
     3841 catcgccagc gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc
     3901 gctggtggca attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacgtt
     3961 aatgggcagc cagccgcaac tggttgctgg gctgctggtg ggcactgttt taggcgtagc
     4021 gctgtttcgc ggcgtaccgg ttggcccgct tattgccgcg ggtctggttt cgctgattgt
     4081 ggggaaacag tagttaatcc cgcaatatat cggcctggcg tttgccccag gcctttctta
     4141 aacatagtaa taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca
     4201 gtatcgcctt tcactaatcc ctgaagcgcc ataatcagct gtaactgctg acgccactgg
     4261 cgaaaactca accccgtctc ttttactatc agtcgggcaa gattgcgttc actcatcgca
     4321 aaaaagccag cccattgccc caatgacccc cactcgacgg gccctttcgc catcatctcc
     4381 accatcgtgc ggattttagg atgagaagaa acgggcaggt gcagctgttg ttgtggctgc
     4441 tgagggagtt catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga
     4501 gtcgtcgtcc tgtttgccag ggttaaaatc aattcccggc ataagggcga tattttcaat
     4561 gtgcaacatg ttgtcggcat tgtcactgcg ctgggttcga taaacagaaa acagagttca
     4621 gcgttagctg tcacttgatt gctatgttcc accccgcccg gtatccaaac cgcatattgc
     4681 ggcggcacca tccacaaagc attttccacc gtacaggtaa ttgcaccatg tagcgccagt
     4741 atcagctgcc ctttgcggtg ctgatgtaca ggactggtta actcatccgt tccggcgtga
     4801 atacaaaatg cgaccgcggc ttcatggtgt cggtcgggtt catagccatt gagattcaac
     4861 ctatgcatca ttttgtccga atttagcgat aatttgtcat tttagcttga ttcaacataa
     4921 caataaaaac ggtaaggtac agcctcgttt gtaacaatga gaagcatatg acctgttcaa
     4981 cttcattaag cggcaaaaac aggattgtcc ttatcgctgg cattctaatg attgccacaa
     5041 cattacgcgt cacctttacc ggcgcagcac cgttactgga tacgattcgt tccgcttact
     5101 cgctgacgac ggcgcaaacc ggcttattga ccaccctgcc attattagcc tttgcgctaa
     5161 tctcacctct ggctgccccg gtggcgcgac gttttggtat ggaacgtagc ctgtttgccg
     5221 cgttactttt gatctgtgct ggtatcgcaa ttcgctctct cccttcgcct tacttattat
     5281 ttggcggtac agcggtcatt ggcggtggaa ttgcattagg caatgtctta ctgccaggat
     5341 taattaaacg cgatttccct cattccgtcg ccagacttac cggcgcatat tccctgacaa
     5401 tgggagctgc agcggcactg ggatcggcta tggtcgtgcc gctggctttg aacggttttg
     5461 gctggcaagg cgcgttgctc atgctgatgt cttttcctct gctggctctt tttttatggc
     5521 tgccacagtg gcgaagtcaa caacatgcaa atttgagtac ctcacgcgcc ttacatactc
     5581 ggggtatctg gcgttctccg cttgcctggc aggtaacatt gtttcttggg atcaactcac
     5641 tggtctatta cgtgattatt ggctggcttc cagcgatcct catcagtcac ggctatagcg
     5701 aagcacaggc gggttcactg catggtttgc tgcaactagc cacagcagct cccggtttgc
     5761 tgatcccact tttcttacat catgtgaaag atcagcgtgg tattgcagcg ttcgttgcct
     5821 tgatgtgcgc agtgggcgcg gtcgggctct gctttatgcc agcgcacgcg atcacctgga
     5881 ctctgctttt cggttttggt tccggcgcaa caatgatact ggggttgacg ttcattggtc
     5941 ttcgggcaag ttctgcgcat caggcggcgg cactctcggg gatggcacaa tccgtcgggt
     6001 atttgctggc agcctgtggg ccgccgctga tgggtaaaat acacgatgct aacggtgact
     6061 ggtccgtacc actgatgggg gttgcaatac tttcactact gatggcgatt ttcggacttt
     6121 gcgccgggag agacaaagaa attcgctaat atccggtgct atagtgacgt gaccaatcat
     6181 gcgtggaagg gagaacaaac acgatgaata ttcagtgcaa acgcgtttat gatccggcag
     6241 aacagagcga tggttatcgc gtactggtcg accgcctctg gccgcgtggt atcaaaaaaa
     6301 ccgatttagc ccttgatgag tgggataaag aaatcacgcc gtcaacggaa ctgcgcaaag
     6361 cctttcacgg cgaagtcgtc gattttgcaa cctttcgcga gcaatatctt gcagaactgg
     6421 cgcaacacga gcaagaagga aagcggctgg cggacatcgc caaaaaacag ccgctgaccc
     6481 tgctttactc agcaaaaaac accacgcaga accatgcgct ggtgctggcc gactggctac
     6541 gtagcttgtg attttagtac agcatccggc ggttattttt caccagccgg atggtcccgt
     6601 cgccacaatg cccattcatc aatcgtttca ccgcccggta atttgcaatt gttgctgacc
     6661 ccttgcgttg tctgcactgg aatgagcgtc ccgcccttct gctggcaata gaccgacgcc
     6721 ggatttgcca taccaatctg cggcggttta ggtgcttctg gctgagaaga ggttgaacaa
     6781 ccagccagaa ccagcaagca aggcagaaca aaactgataa ttttcattta ttgatctcac
     6841 atatttatcc aggcttagag tatcgcggta tcgttttgtt ttgcagcact atttttatta
     6901 cattcactca aatcatatta cgccttgttt catctttgtt gatgatgttt tatcatgcct
     6961 gcaaagatta aataatcagc atttacccgc cgtatcctgg agttgtttcg tgtcagatca
     7021 gattatcgcc cgcgtctcgc aatcccttgc caaagaacag tcactggaaa gcctggtccg
     7081 acagcttctg gagatgctgg aaatggtcac tgatatggaa tcaacctacc tgaccaaagt
     7141 ggatgtcgaa gcgcgcctgc agcatataat gtttgcccgt aacagccaga aaatgcacat
     7201 cccggagaat tttaccgtct cgtgggatta ctcgttatgc aaacgcgcca ttgatgaaaa
     7261 ctgctttttc agcgatgaag tccccgaccg ttggggcgac tgtattgcgg cacgcaatct
     7321 tggcatcacc acatttctga gcacgccaat tcacttaccg gatggatcat tctatggcac
     7381 gctttgcgcc gccagcagtg agaagcgcca gtggagtgaa cgcgcggaac aggttttgca
     7441 gttattcgcc ggactgattg cacaatatat tcaaaaagag gcgctggtag aacagctgcg
     7501 cgaagccaat gccgcgctga tagcgcaatc gtataccgac tcgttaaccg ggttaccaaa
     7561 tcggcgggcg atttttgaaa atctgacgac gctgttttct ctcgcccggc atcttaacca
     7621 taagataatg atcgcgttta tcgacctgga taacttcaaa ttaatcaacg atcgttttgg
     7681 tcataatagt ggcgatctgt ttctcattca ggttggcgag cgccttaata cgctccagca
     7741 aaatggcgaa gttattggtc gtctcggcgg tgatgagttt ttggttgttt cactaaacaa
     7801 cgagaatgcg gatatttcgt cgctgcgaga acgcattcag cagcaaatac gcggagaata
     7861 tcacttaggt gatgttgatt tgtattatcc tggtgccagt cttggcatag tagaagtcga
     7921 tcctgaaacg accgatgcag acagtgccct acatgctgcc gatatcgcga tgtatcagga
     7981 gaaaaaacac aaacagaaaa caccttttgt cgcgcattca gcgccacatt cctgaggcgt
     8041 attcacatcc ttttgattgg tgataacatg cgaatcggta ttatttttcc ggttgtaatc
     8101 ttcattacag cggtcgtatt tttagcatgg ttttttattg gcggctatgc tgccccggga
     8161 gcataaagat gaaaaaaaca acgatcatta tgatgggtgt ggcgattatt gtcgtactcg
     8221 gcactgagct gggatggtgg taacgtcaca tctaaaaaat agcaaaggct gcctgtgtgc
     8281 agcctttgtg caatttacgc gttaactttt aatcttcctg tagataaata gcacgacaat
     8341 cgcaccaata acggcaacca cgaagctgcc aaaattgaag ccatcgactt taccaaagcc
     8401 aaacagcgtg ctgatccaac cgccgactac ggcaccgact atccccagca ggatagtcat
     8461 aaagaatcca ccaccatctt tacctggcat gatccacttc gccagaatac cggcaataag
     8521 cccaaaaata atccatgaca gaatgcccat tgtttcctca cttatctgtt ttgcattagc
     8581 ggggtagtcg ctgataaaaa gcatagcaca acatcgggag ggcaagattt gtgacgagca
     8641 tcacggagga tttttttgcg atggcgcaga aattgcgcca tcaacgatca gtgataatta
     8701 ccaaccacaa acatcatgtt cgttttccgt gtcataagaa cgcacggtat tcaccagatc
     8761 ttttatcact tcagccgcca cttctggcac cagcaaagtc atcggcgtct ctgtttcata
     8821 atcgacagaa acgccattgc tgttattggt gacggtcacg gtatacgttg ctttgcccat
     8881 gattcatttc ccgttatgaa tgactttccg ttgttgcgca ccttccatca ggacttcagg
     8941 agccacgaag aagtcaatgt tgaaataagt atcgtcagtc atggcttcaa tgttgtgcca
     9001 cttttctgga gggaacaccg caaactgccc cgcttcgata aggatcacct gatcaggctc
     9061 tgcactgtgt tcatcagcgt agccgagata tttgaccgcc ccatgcataa cggaaaggcg
     9121 tgggtaaacc cccgggcgcg ttcctttatc aagatgacgt tcgaatattc cggcaggcgc
     9181 agtttgttta ttccagaaag gcgttgagcg cgtatgaata taattctgtg ggatttgcag
     9241 catccttttc cctccttcgg tgaatgcgct gaaaacggtt tattccagcc gtttcagggt
     9301 acgcctgata atttgcattt taaataccat ttattggtta ttttttagca ccatatcaga
     9361 gaagaatcag ggaggattat agatgggaag cccgcgacaa cgcgggctga aagcatcagg
     9421 attgcagcgt cgccagtcgg gcagcgaaac ccacgaacat caaaccaatc agtgagttgc
     9481 ccactttagc cagtttcttt ttggtacgta tgtactgcgt gacaaaagca ccagatataa
     9541 tcaggaagct caaatagcag aaactcacca gttccagcgt cgtcgccaga ataaagaatg
     9601 aaattcccgt atgtggggca ttaacatcga taaactgtac gaaaaacgac acatagaaca
     9661 aaatggcttt cggattagtc aggctcaaaa ttaacgcgcg tttaaaaatg gcaccgtatt
     9721 ggggctcatc ggatttggcc tcgttatttt tacccttcag ggtcgcgtag agaattttac
     9781 tccccagata gagcaaataa aatgcaccaa gataacgtac aatgttgaat aatatcgggg
     9841 tggtcttaat taatgtcgcc actccagccc atgccagaaa catcaatacc gcatcgccaa
     9901 taaatacacc gcaggccgca agataaccgc ctttcatacc gctactgacg ctatttttga
     9961 gtacaaacag ggtatttggc cctggcacca acacaataaa aatggcccca accagatagg
    10021 tccagtaatt cagaaccccg tattcagcga acacattaac ctctttaatt atctttcgat
    10081 catgcgcgat taaaggtgaa tatgctaacc aatctgtagc agcttagaaa ggagaaaatc
    10141 aggttttaac ctgatatcaa cccgataatt gaatcattaa tcaggcatgc tccagtgaaa
    10201 aattcgggta gtgctcggca aaatactggc gtaaaaattc taccgttatc cgcactttcg
    10261 ctgacgtcgc cagccttgaa acataaacgg cccagacgtt cgctggctgg taatattccg
    10321 gtagcacttg cactaaatga ccactggcaa tgttttcgct aacatcccac caggaacgca
    10381 gagcaatccc ctgcccatcg agacaccact gatggacaat ttctccatga ttagacgaca
    10441 gtgggccggt gactttaatc gcatggtggc cttctttatt gcgtaattgc cagacaccaa
    10501 aaggatgatc gcgctcttta atgaccaggc acggcaatgc agaaagatcg gttaaatgtt
    10561 tcggcgcgcc atgttgcgca ataaattccg gcgacgcgca gagaatgcgg taattggtcg
    10621 ccagtttgcg ggcgattaaa ttaggggcga tgtcatcgcc aatgcgaata tcgagatcga
    10681 caccttcatt gaccaaatcg accagccggt cttccacatc aaaacgtaat tccagttgcg
    10741 gatacgcctt cgccagcgct gataacgccg gagccaccac ctgtcgtcca aaaccaaagc
    10801 tgctgataat acgcagcatc ccctgtggca cctgacgcac gtcagaaagt tcgtccatca
    10861 tctgaccgac atcctgcaaa atcagctgcg cccattcata aatccgctct ccttcttcgg
    10921 taatggtgac gcggcgggtg gtgcggtgta acaacaccac gtttagcgtt tgctccagca
    10981 aggcgatgcg cttgctgacg aacgccggtg aaacgcccag ttcttccgcc acggtggcaa
    11041 aaccagcccg gcgagccacc agcataaaga cgcgcaaatc attcagcagc ggtaaattat
    11101 tcatgattcg tgttttatgt ttcaccagtt acggggatta attccttttc agtcaattat
    11161 aggatggtga tgttgtcaat tttgatggtc aggaagtgag aacccaatga tgaaaacgat
    11221 gcgtattgct gcgatcccgg gagacgggat tggcaaagaa gtccttcctg aagggattcg
    11281 cgtgttacag gctgccgctg agcgctgggg cttcgccttg agttttgagc aaatggagtg
    11341 ggcgagctgc gagtattaca gccatcacgg taaaatgatg ccggacgact ggcatgagca
    11401 acttagccgt ttcgatgcca tctattttgg tgccgtcggc tggccggaca ccgttccgga
    11461 ccatatttcg ttgtggggtt cgctgctgaa atttcgtcgt gaattcgacc agtacgtcaa
    11521 cctgcgcccg gttcgtctct ttcctggcgt tccctgcccg ctggcgggaa aacagcctgg
    11581 cgacatcgat ttttacgtgg tcagggaaaa caccgaaggc gaatattcct cgctcggcgg
    11641 tagagtgaat gaaggtacag agcatgaagt cgtcattcag gaatcggtat ttacgcgtcg
    11701 tggtgtcgat cgcattttgc gttatgcctt cgaacttgcg caaagccgcc cacgtaagac
    11761 gctaacttct gctactaaat caaacggttt agccatcagc atgccgtact gggatgagcg
    11821 agtggaagca atggccgaga attacccgga gatccgctgg gacaagcagc atattgatat
    11881 tctctgcgcg cgttttgtga tgcagccgga acgcttcgat gtggtggtgg cgtccaattt
    11941 gtttggcgat atcctttccg atcttggccc ggcctgcacc ggcaccattg gcattgcccc
    12001 atccgccaac ctgaatccgg aacgcacttt cccgtcgctc ttcgagcctg tccacggttc
    12061 cgcgccggat atctacggga aaaatattgc taaccctatc gccacaattt gggccggtgc
    12121 aatgatgctc gattttctcg gcaatggcga tgagcgtttc cagcaagcgc ataacggtat
    12181 tctggcagcg attgaagaag tgattgctca cgggccgaaa acaccggata tgaaaggcag
    12241 tgccaccacg ccacaggttg ccgacgcgat ttgcaaaatt attttgcgtt aaggtcaaac
    12301 cagtttattt gaaccgcgtc actgacgcgg tttttttatt cgttctttgc agttaataac
    12361 ctgcgtcatt tcacctttta ttgtttccgt ttcgtgtttt atggctttcc gtattcttaa
    12421 ttgtttaatt tatgtaacat gcaaataatc aatttgttaa acacaacata caactcatta
    12481 ccctgcaaat aattacaact ggcaaaagcc agagacgggc ttttatgccc tggagatgac
    12541 tcatgatgag caatgttaag aaaaaagatg tgccgctgat aagcatcagc ctggtggcca
    12601 ttcttttcat cgcagctgca ttaagccttt tcccacaaca atcggccgac gcggccaacg
    12661 caatatacac ttttgttacg cgtacgttag gttccgccgt acaggtattg gttttgctgg
    12721 caatgggact ggtgatttat ttagccacca gtaaatacgg caatattcgt cttggcgaag
    12781 gaaaaccgga atacagcacg ctctcctggc tgtttatgtt tatttgtgcc ggtttaggtt
    12841 cttctacgct ttattggggg gttgctgaat gggcctatta ttatcaaacg cctggattaa
    12901 atatcgcacc gcgttcacaa caggcactcg aatttagcgt cccttactcc ttcttccact
    12961 ggggcatcag cgcctgggca acttatacgc tggcctcatt aatcatggct tatcactttc
    13021 atgtgcggaa aaacaaaggt ctgagtcttt ccggcattat tgccgcgatt accggcgttc
    13081 gcccgcaagg cccatgggga aaactggttg atttgatgtt cctgatcgcc actgtcggcg
    13141 cactgactat ttcccttgtt gtcaccgcag caacctttac tcgtggactt tccgcactga
    13201 ccggtttacc cgataacttc accgtgcagg catttgtgat cctgctttcc ggcggcattt
    13261 tttgcctaag ctcatggatt ggtatcaaca acggtttgca acgtctgagc aaaatggttg
    13321 gctggggcgc gctcctgctg ccattactgg tgctgattgt cggtccaacc gaatttatta
    13381 ccaacagcat catcaatgcc atcggcctga ccacgcaaaa cttcctgcaa atgagcttat
    13441 tcaccgatcc gcttggcgat ggttcattta cccgcaactg gaccgttttc tactggctgt
    13501 ggtggatctc atacacccct ggcgtagcaa tgtttgtcac ccgcgtttcc cgcggtcgta
    13561 agattaaaga agttatctgg ggactgatcc tcggcagcac cgtcggttgc tggttcttct
    13621 tcggcgtaat ggaaagctat gccattcatc agtttatcaa tggcgtaatc aacgtcccac
    13681 aggtgctgga aactctgggc ggcgaaacag ctgtacagca agttctgatg tcgttgccag
    13741 ctggtaaatt gttcctcgcc gcatacctgg gcgtgatgat tattttcctt gcctcgcata
    13801 tggatgcagt ggcctacacc atggcggcga ccagtacgcg taatctccag gaaggtgacg
    13861 atcctgaccg tgggctgcgt cttttctggt gcgtggtgat cactctgatc ccgctttcca
    13921 tcttgtttac cggtgcttcg ctggaaacga tgaaaaccac cgtcgtgctc acagcccttc
    13981 ccttcctcgt cattttactg gtgaaagtcg gcggatttat tcgctggctg aaacaggatt
    14041 acgccgacat tccggctcat caagttgaac attatctccc gcagacaccg gttgaagccc
    14101 tggaaaaaac accagtgctc cctgcgggaa ccgtattcaa aggcgacaac tgagcgccat
    14161 catcctaacg ataaaggtat ccctatgagc aatctgagcc ctgactttgt actacccgaa
    14221 aatttttgcg ctaacccgca agaggcgtgg accattcctg cccgttttta taccgatcag
    14281 aacgcgtttg aacacgaaaa agagaacgtc ttcgccaaaa gctggatttg cgtcgctcac
    14341 agcagcgaac tggcgaatgc caatgattat gtgacgcggg agatcattgg cgaaagcatc
    14401 gtgctggtac gcggtcgtga taaggttttg cgcgcgttct ataacgtgtg tccgcaccgt
    14461 ggtcatcagt tgttgagcgg tgaaggaaaa gcaaaaaatg tgattacctg cccatatcac
    14521 gcatgggcat tcaaactcga tggcaacctg gcccatgcac gtaactgcga aaacgtcgcc
    14581 aatttcgata gcgacaaagc gcaactggtt ccggtgcgtc tggaagaata tgccggattc
    14641 gtcttcatca acatggaccc caacgccacc agcgtagaag atcagttacc cggtctgggc
    14701 gcgaaagtgc tggaagcctg cccggaagtc cacgatctga aactggcggc ccgctttacc
    14761 acccgcacgc ctgccaactg gaagaacatt gtcgataact atctcgagtg ctatcactgt
    14821 ggtccggcgc atccaggttt ctccgactcc gtacaggttg atcgttactg gcacaccatg
    14881 cacggtaact ggacgctgca atacggtttc gccaaaccgt ccgaacagtc gtttaaattt
    14941 gaagagggta cggatgcggc attccacggt ttctggctgt ggccgtgcac gatgctgaac
    15001 gtcaccccga tcaaagggat gatgacggtc atttatgaat tcccggtgga ttctgaaact
    15061 accctgcaaa actacgatat ttacttcacc aatgaagagt taaccgacga gcaaaaatcg
    15121 ctgattgagt ggtatcgcga tgtgttccgt ccggaagatt tacgtctggt tgaaagcgta
    15181 cagaaagggc tgaaatcgcg tggctatcgt ggtcaggggc gcatcatggc cgacagtagc
    15241 ggtagcggca tttccgaaca tggtatcgcc catttccata atctgctggc gcaggtgttt
    15301 aaggactaat gacatcggcg gcggtatttt ccgccgctgg gctgattttt gatggagtac
    15361 agcaatgtca gactatcaaa tgtttgaagt acaggtgagc caggttgaac cccttaccga
    15421 acaggtgaaa cgcttcacgc tggtggcaac cgatggcaaa ccattacctg cgtttactgg
    15481 aggaagtcac gtcattgtgc agatgagcga tggtgataac cagtacagca atgcgtattc
    15541 actgctgagt tcgcctctcg atacaactca ttatcagatt gccgttcgac tggaagaaaa
    15601 ctcgcgcggc ggttcccgct ttttgcatca gcaggtaaaa gtgggcgatc ggttaacgat
    15661 ctcaacgcct aataacctgt ttgcgctaat tccctcagcc agaaagcatc tgtttatcgc
    15721 gggcggtatt ggtatcaccc ctttcctgtc gcacatggca gagttgcaac acagcgacgt
    15781 cgactggcag ctacattact gctcgcgaaa tccagaaagt tgcgcatttc gtgatgagct
    15841 agtccagcat ccgcaggctg agaaagtcca tttgcatcat tcatcaaccg gaacacgact
    15901 ggaattagcg cgattattgg cggatatcga acctggcaca cacgtttata cctgtggccc
    15961 cgaggcgcta attgaagcgg taagaagtga agctgcgcgt ctggacatcg ccgccgatac
    16021 gctgcacttt gagcaatttg ctatcgaaga caaaatcggc gatgcattta ccctggtgct
    16081 tgcccgttcc ggaaaagagt ttgtggtgcc ggaagagatg actattttgc aggttattga
    16141 aaataataaa gccgcgaaag tggaatgttt atgtcgtgaa ggggtatgcg gaacctgcga
    16201 aacggcaata ctggaaggtg aagctgacca tcgggatcag tattttagcg atgaagagcg
    16261 tgccagccag caaagtatgt tgatctgttg ttcgcgtgcg aagggtaaac gcctggtgtt
    16321 ggatttgtag tttgccgaag ccggatgtgg cgctgagcgc gcccagtccg gcttcggaag
    16381 attttactgc ggatattcct gcaataaatt gtgtaatgct tccgccatca gctcaccacg
    16441 ccagccggaa atcagctccg gcaaattgtt ctgcggtttc agtttccagt gccagttcag
    16501 cagttggttg atttgccgac gcgatgccag caattcggcg ctgatcttgt gcgtttcgct
    16561 cacgtcagta atcagcgact taatcgcttt aaacgcttta cgataacccg gcatgtccat
    16621 caggttaagc atcggctgcg gtaaggcctc ttccggcaat gtctgcgctt tttccaccag
    16681 cgccagcagc gttttaccgt gaaagcggat ttcgctaccg gataaaccca ggctgtccag
    16741 ttcgcctaaa cttcccggca tataacgcgc taccgaccac aaatgctctt cacgcacgac
    16801 aaagttcacc gccagatcgc gctcacgcgc cttgcgcagt cgccagtcgg ctaacagttg
    16861 cagacaggcc agttggcgtg tgcgtaattg ccaggcattg gtgatatcac gccaggcatc
    16921 ttccggcgca acgacttcct gacgacgcat ttgcatcagg cggcattcat ccagcgccgc
    16981 aggtagccag ccggaggcct ccgtttctac cataagcttg gcggtgatcg gtaacagata
    17041 ccagacatcc gccgctgcat attcacactg acgttcggtc agcggtctgg ccagccagtc
    17101 ggtgcgcgat tcactcttgt ccagcgtaac gccggaatac tcttccacca tggaagcgaa
    17161 accccatgac atcgggcgtc cgcagaaggc agcaaggatt tgcgtgtcaa tcaggggttg
    17221 tggtaattcg ccaaagacat tgaggaacac ttccagatct tcactgcctg catggagaaa
    17281 ttttgtgatg gacggatcgc gcaggatcgc tttcagcggt gaccagtcgg tgatcccgag
    17341 aggatcaatt agcgccagat gctcaccatc aaaaagttga atcaacccca gctgcgggta
    17401 ataagtgcgc gtacgaacaa attcagtatc cagggctatc gccggaaagg cacggacggc
    17461 ttcacacaaa gaagccagcg catcgtccgt ggtaatcatt tggtaattca aattgtattc
    17521 tctttagtgg gcgtcaaaaa aaacgccgga ttaaccggcg tctgacgact gacttaacgc
    17581 tcaggcttta ttgtccactt tgccgcgcgc ttcgtcacgt aattctcgtc gcaaaatttt
    17641 tccgacgtta gatttcggta actcatcacg aaactccacc agcttcggta ctttgtagcc
    17701 cgtgagctga cggcggcaaa aggtcaccag tgactcttcg gtaagcgatg gatctttttt
    17761 cactacaaag attttcaccg cttcaccact ggagccggaa ggtacgccaa cagccgcgac
    17821 ttcctgtacg ccaggatgct gcatgacgac atcttcaatc tcgttgggat agacgttaaa
    17881 accggaaacc agaatcatgt cttttttacg atcgacaatg cgcaggaacc cttcttcatc
    17941 catcaccgcg atgtcgccgg tgtgtaacca gccatttttg atgatctcat cggtagcatc
    18001 cggacgctgc cagtaaccca gcatcacctg cggtcctttg acacaaagct cacccggttg
    18061 acccggtggt acttcattat catcatcatc caccagtttg gcttccgtcg acggcaccgg
    18121 caaaccgatg ctaccactat gataatcaat atcatatggg ttaacgctga ccagcggcgc
    18181 acactcggta aggccatagc cttccagcag atactgtcct gtcagtttca cccaacgctc
    18241 tgccaccact tgctgcactg gcatcccacc gcctgcggaa agatgcagac tggagaaatc
    18301 cagctgctgg aactctttat tgttcagcaa cgcattgaac aaggtgttaa cgccagtgat
    18361 agcggtaaac ggatatttcg ctaactcttt taccaaccct ggaatatcac gcgggttagt
    18421 gataagcagg ttctgcccac ccagttcgat aaacagcagg cagttaatgg tcagggcaaa
    18481 aatgtgatac agcggcagtg ccgttactac cagttcttta cctggatgca gcaacggccc
    18541 ataggtggcg ttgacctgtt ccaggttcgc cagcatattg cggtgagtca gcatcgcgcc
    18601 tttcgccaca cctgtggtgc cgccggtgta ttgcaggaag gctaaatctt ccggcaccag
    18661 ttcgggtttg acgtactgca tccggtagcc gttatgcagg gcgctacgaa atgaaatggc
    18721 atctggcaga tggtatttcg gcaccaaacg cttgatgtat ttaacaacga aattgactac
    18781 cgtgcctttt gccgtagata gctgatcccc catacgggtc agaattacgt gctgaacggc
    18841 ggttttatca accacttttt ccagtgtgtg agcaaagtta gacacaataa caatcgccga
    18901 tgcgccgcta tcgttaagct gatgctcaag ctcacgcggg gtatacaacg ggttaacgtt
    18961 tacgacgatc atcccggcac gcaaaatgcc aaacagcgcc accggatatt gcaataaatt
    19021 aggcatcatc aacgcaacgc gatcgccttt cttcagcccc aacccttgtt gcaaataagc
    19081 ggcaaacgcg cgactgcgtt cttccagctt gcggaaggtc atcacctccc ccatattcac
    19141 aaacgcaggc tgatcggcgt agcgcgcgac cgactgctca aacatatcta ccagagattg
    19201 ataacggtca gggttgatct ccgtcggaac gtccgcggga taacggttaa gccaaacctt
    19261 cttcaattct tcacctctaa aatgcgtgtt cgtcgtcatc gcaaccccaa atgatataca
    19321 tgccgttaac ataatattaa ctcatcatac cagcttgata attacccaac gaaaaggttg
    19381 cgaagcgcgt cactatttat ttttatcttt accgtaagaa tgcagaaaca gcggaccagc
    19441 cgctgtttct ttttctttaa aaacaagcaa ttattcagtt acaactgttt gtactctcgc
    19501 ggggccggga ttataccagc cccatccgcc gtagccatag ggccagccac gaccgccata
    19561 aaaccatgga tcaatcggct gaggcggcat aatcacctgc tgggttaaat gccaacgttt
    19621 gtaacccgtt acttgcatca ccataaattt atagggcgta ttgccgattt tgccgtcaac
    19681 cgcaccagtg attggcccga ctaccgtaac cagttgtcca cggaaatcca ccgggtccag
    19741 aaaaccgttc acatcggcat aaatgcgacc gcgagaaggt tctcccagcg tcggtctggc
    19801 tccgctgtcc agcggtacgg tagcaatttc caggcgggtt ttcccttgct ggttttgtac
    19861 cgcaaccact ttgccaccaa agcgtgcctc ctgaccaacg tacagctgcg gcgcactcat
    19921 cacccgaact aaatcttgtt gcggcgtggg actactgcct ttaatggcgt ccggcacagt
    19981 gacacaaccg ctcagcatta gcgcaaacgt acctgccagt atgcctttga taacattctt
    20041 ttgaaccgcc atgatgcgac tccttttttt ctcagggcat actcttaaga ttcattcttt
    20101 gcccggaagt ttcttccatg cgacgttgtt acgtaaataa accggttcgg catgttccac
    20161 cgccaccgtt ttaccctcgg caaacatctg acaagcaatc ggcagcatat cttctgcagc
    20221 gggcagtaaa acttctccgt cgcgcaaaac cagtccgctc tctttaccga gatccggcca
    20281 ggcttgccag cctgtaccta ccgtcaccca ttccccggaa agctgttgca tccgttcatg
    20341 aacgagttcg ggtttgagta cggcttcggt ttcttcgccg tgccagacgc cgttttcatc
    20401 acgctgatat tcggcccagt aaacttcgcc catccgcgca tcaatggcag ccagcacgcg
    20461 cgttgcgccg tttttgcgcc acgccccctg cgccatcgtc atgagcgtgg agacgccaat
    20521 catcggcagc gccgcgccaa gtgccagccc ctgcgcaatg cctataccaa tgcgcacgcc
    20581 ggtaaaacca ccggggccgc gcccgtaggc cagagcatca atatcagaga gggaggtacc
    20641 gctggtggtc aggatatcct gtaccatcgg taagattcgt tgagtatgtt cacgagggca
    20701 aagctcaaaa tgagcgttga cagtaccgtc gttccacagg gcgacagagc aggcctctgt
    20761 cgcggtatcg atagccagaa ttcgcatggg tcttcgtgct tagatcaata aaaaggcgcg
    20821 catcatacca tactccgtaa caaattacct ggaggatggt atcgcaagga aacgaaccgc
    20881 acgggcaatg tcacgggtgc gcggcgcggg cggcagactg gcgagaaacg tcgcaccgta
    20941 agggcgcatc acaagccgat tatcgcaaat caccagcacg ccgcgatcat cggcgtcgcg
    21001 aatcagtcgc cctaccccct gcttgagagt aatcaccgca tccggcagtt gtacttcatc
    21061 gaacgggtcg ccaccgcgca aacggcaatc ttccatgcgc gcttttaaca gtggatcatc
    21121 aggagaggta aacggcaatt tgtcgataat caccaatgac agcgtatcgc cacgcacgtc
    21181 caccccttcc cagaaactgc tggttgccac aagaagcgcg ttaccggcgc tgacaaactg
    21241 ctgcaacagt tgccctttgc tggtttcccc ctgcaacaaa acgggaagcg tcatggtagc
    21301 gcggaactgc tcggccagat cgcgcatcat ggcgtgcgag gtacaaagca taaaacaacg
    21361 accgttgtta gcttcgatga tcggtcgcag catagccgcc agtttccgcg cggaacccgg
    21421 ttgattggtt tgcggcaggt tgcgcggcac acagagtaac gcctggcggc tgtaatcaaa
    21481 cgggcttggc aatagcaacg actccgcctg ttctatccca agccgcgagg tgaaatgatg
    21541 cagatcgtcg ttcaccgaca gcgttgctga ggtgaagatc cagctaccgg gcttttgcgc
    21601 cattaactct ttgaatttat ccgccacgct gagcggcgtg agagccagag taaaatggcg
    21661 cgaagtgcat tcgtaccagt agctgtagcc cggctgattg atctctttta gccgcttcag
    21721 ccgtgtgcga tacaacgtgg cgcgctcaaa tgccgcatcc agcaaggcag aacgccccag
    21781 tgacagtttc gccacgtcat aacaaagttc cagggtgtca tcgagcagta aaaatgcccg
    21841 ctgaatttgc ggattagcta acagctcacg caggttaccg cgataacctg gttcaccgag
    21901 ttgcagacga aaatcctgcg cactctgagc aagacgatct gcgcactttt gtaactgctg
    21961 ggtgtctttt aattcggtgc ggtaggcgat ggtgatgtct tttgccaggt cgagcagttg
    22021 tcgactggag agtgactgac caaaatactg gctggcaatg tccggtagct ggtgggcttc
    22081 gtcgaagatc atgacgtccg cttccgggat caactcgcca aatccactct ctttaaccac
    22141 catatccgcc agaaagagat gatggtttac caccaccaca tcggcgtcca tcgctttttt
    22201 acgtgctttg accacaaagc aatctttata catcgggcag tcgctgccaa gacagttgtc
    22261 gttggtgctg gtgaccagcg gccacgcctg cgaatcttcc gccacgctga cgcaggtgct
    22321 gatatcacca tcgactgttt gattagacca ggagcgcagc aggatcacat cgcttaatat
    22381 ttgtaccgga agatcgcccc ccgccagcgc ctgctgttcg agacgttcga ggcagaggta
    22441 gtttgagcgc cctttcagca acgccacgtt gcccgtgtat ttcaacgcct ttgagaccgt
    22501 cggcaaatcg cggctataga gctgatcctg caacgctttt gagccggtcg agataatgac
    22561 tttctttttc gcccgcagcg caggggccag gtaagcgtag gttttgcccg taccggttcc
    22621 tgcttccacc accagcggct ggcctttttc tatcgcctgg gtgacggcta ccgccatctg
    22681 tcgctgtggt tctcgcggct taaagcctgg tatcgctttc gccagctgac cgtctggtgc
    22741 aaaatcgtcc gtcacactac cccctgttga tttgaacagg gattatgtca ggatgagggc
    22801 gctttcgcca gttgaagtgg tgacggcgac ctcacattgt ggcagtcttt gcagcacgaa
    22861 aatggaagga taatgaggac aaaatgatga ctatcgttcg tatcgatgct gaagcccgct
    22921 ggtctgatgt agtaatccac aacaacacgc tctactacac tggtgtaccg gaaaacctcg
    22981 acgccgatgc ctttgagcaa accgccaaca cgctggcgca gattgacgcc gtgctggaaa
    23041 aacagggcag caataaatcg agtattctgg atgccaccat tttcctggcc gataaaaacg
    23101 acttcgcggc gatgaataaa gcgtgggatg cctgggttgt cgcgggtcat gcgccggtgc
    23161 gctgcacggt acaagcgggt ttgatgaacc cgaagtataa agttgaaatt aagattgtgg
    23221 ctgcggtgta agctttatcg aagcaaaata agtcagacga taatttatcg ataatactgg
    23281 tcggttttac ataaatcgac cagagaataa gatattactg ttgatgcttc tgctaattca
    23341 ttatttattt ttatagtttc aattttatct ataaaactaa ctatataaat aaacataacc
    23401 atgccgttgt gttcgccctt tgattagcaa ctctggtttt ctttcttagc tgaacaatct
    23461 ggttatagcg tgcggactga ccgttaattt tctccatctt actggataac acaccatgag
    23521 ctgctacctt taaaacacct gaatgtcaac aggttaactc gtgccatatc gttataatcc
    23581 ctttgatatt agatgcaaat taaggtcata tatagcctta tcgataacat ggttaatttt
    23641 aaggataaga atatgcctgc tgtaatagat aaagccctgg atttcattgg tgccatggat
    23701 gtatcagcgc caacaccaag ttcgatgaat gaaagcacgg cgaagggaat ctttaaatat
    23761 ttaaaagaac tgggagtacc cgcgagtgcc gctgatatta cggcgcgagc tgaccaggaa
    23821 ggctggaacc ctgggttcac agaaaaaatg gttggatggg caaaaaaaat ggagacaggt
    23881 gaacgctctg tgattaaaaa tcctgagtac ttttcaacat atatgcagga agaactaaaa
    23941 gcactggtct gagttaaatt tatatcagca taaatgggtc aggacgcttt taatcacata
    24001 taaaaagcgt cctttttctc acaatcaaat aatcatcggc ttctataacg aaatcgatac
    24061 ggattagtgt gttactcgtc ttcatcctca aaacgcgcca cgattcgctc gccggagtgg
    24121 ttggcacgca gctcttctgc caccagcgcg attgcctggc cgctgctcat cccttgtgcc
    24181 atcagttcct ggatccgctc gacagctttt tgctgctgtt catgggtgag tgaaggtaaa
    24241 cctgcaaaca ttgttaactc ctgctaaatt gttggcgcta attatttcat gctacccggc
    24301 acatagccag tagagtcagg actgatgaag acgttatctc ccgctgtgat tactttaccc
    24361 tggcgtcagg acgccgctga attttatttc tcccgcttaa gccacctgcc gtgggcgatg
    24421 cttttacact ccggctatgc cgatcatccg tatagccgct ttgatattgt ggtcgccgat
    24481 ccgatttgca ctttaaccac tttaggtaaa gaaaccgttg ttagtgaaag cgaaaaacgc
    24541 acaacgacca ctgatgaccc gctacaggtg ctccagcagg tgctggatcg cgcagacatt
    24601 cgcccaacgc ataacgaaga tttgccattt cagggcggcg cactggggtt gtttggctac
    24661 gatctgggcc gccgttttga gtcactgcca gaaattgcgg aacaagatat cgttctgccg
    24721 gatatggcag tgggtatcta cgattgggcg ctcattgtcg accaccagcg tcatacagtt
    24781 tctttgctga gtcataatga tgtcaatgcc cgtcgggcct ggctggaaag ccagcaattc
    24841 tcgccgcagg aagatttcac gctcacttcc gactggcaat ccaatatgac ccgcgagcag
    24901 tacggcgaaa aatttcgcca ggtacaggaa tatctgcaca gcggtgattg ctatcaggtg
    24961 aatctcgccc agcgttttca tgcgacctat tctggcgatg aatggcaggc attccttcag
    25021 cttaatcagg ccaaccgcgc gccatttagc gcttttttac gtcttgaaca gggtgcaatt
    25081 ttaagccttt cgccagagcg gtttatcctt tgtgataata gtgaaatcca gacccgaccg
    25141 attaaaggca cgctaccacg cctgcccgat cctcaggaag atagcaaaca agcagaaaaa
    25201 ctggcgaact cagcgaaaga tcgtgccgaa aatctgatga ttgtcgattt aatgcgtaat
    25261 gatatcggtc gtgttgccgt agcaggttcg gtaaaagtac cagagctgtt cgtggtggaa
    25321 cccttccctg ccgtgcatca tctggtcagc accataacgg cgcaactacc agaacagtta
    25381 cacgccagcg atctgctgcg cgcagctttt cctggtggct caataaccgg ggctccgaaa
    25441 gtacgggcta tggaaattat cgacgaactg gaaccgcagc gacgtaatgc ctggtgcggc
    25501 agcattggct atttgagctt ttgcggcaac atggatacca gcattactat ccgcactctg
    25561 actgccatta acggacaaat atactgctct gcgggcggtg gaattgtcgc cgatagccag
    25621 gaagaagcgg aatatcagga aacttttgat aaagttaata agatattacg ccaactggag
    25681 aagtaagacg tggaataccg tagcctgacg cttgatgatt ttttatcgcg ctttcaactt
    25741 ttgcgcccgc aaattaaccg ggaaacccta aatcatcgtc aggctgccgt gttaatcccc
    25801 atcgtccgtc gaccgcaacc ggggttgttg ctgactcagc gttcaattca tctgcgtaaa
    25861 cacgctggac aagtggcatt ccctggaggt gcagtcgatg acacggacgc atcagttatc
    25921 gccgccgcgc tgcgcgaagc tgaagaagag gttgctatac cgccttccgc cgttgaagtt
    25981 atcggcgtgc tgccgcccgt cgatagcgta actggctacc aggtaacccc agtggtcggc
    26041 attatcccgc ccgatctgcc gtatcgcgcc agtgaagatg aagtctcggc ggtgtttgaa
    26101 atgccgctcg cccaggcatt acatctgggt cgttatcacc ctttagatat ctaccgccgt
    26161 ggcgattcac atcgggtatg gctgtcctgg tacgaacagt attttgtatg gggaatgacc
    26221 gcaggcataa ttcgtgagct ggcgctgcaa attggtgtga aaccctgact atacttatct
    26281 ttacatctac aaaacactac ttgagacaat catcgcaata ttagttaaat cgcggttttt
    26341 gattagttta attcatgtga atagttaagc cagtcgccgc gttccctctt acactatgcg
    26401 ctgttattag ttcgttactg gaagtccagt caccttgtca ggagtattat cgtgattagt
    26461 ctattcgaca tgtttaaggt ggggattggt ccctcatctt cccataccgt agggcctatg
    26521 aaggcaggta aacagttcgt cgatgatctg gtcgaaaaag gcttactgga tagcgttact
    26581 cgcgttgccg tggacgttta tggttcactg tcgctgacgg gtaaaggcca ccacaccgat
    26641 atcgccatta ttatgggtct tgcaggtaac gaacctgcca ccgtggatat cgacagtatt
    26701 cccggtttta ttcgcgacgt agaagagcgc gaacgtctgc tgctggcaca gggacggcat
    26761 gaagtggatt tcccgcgcga caacgggatg cgttttcata acggcaacct gccgctgcat
    26821 gaaaacggta tgcaaatcca cgcctataac ggcgatgaag tcgtctacag caaaacttat
    26881 tattccatcg gcggcggttt tatcgtcgat gaagaacact ttggtcagga tgctgccaac
    26941 gaagtgagcg tgccgtatcc gttcaaatct gccaccgaac tgctcgcgta ctgtaatgaa
    27001 accggctatt cgctgtctgg tctcgctatg cagaacgaac tggcgctgca cagcaagaaa
    27061 gagatcgacg agtatttcgc gcatgtctgg caaaccatgc aggcatgtat cgatcgcggg
    27121 atgaacaccg aaggtgtact gccaggcccg ctgcgcgtgc cgcgtcgtgc gtctgccctg
    27181 cgccggatgc tggtttccag cgataaactg tctaacgatc cgatgaatgt cattgactgg
    27241 gtaaacatgt ttgcgctggc agttaacgaa gaaaacgccg ccggtggtcg tgtggtaact
    27301 gcgccaacca acggtgcctg cggtatcgtt ccggcagtgc tggcttacta tgaccacttt
    27361 attgaatcgg tcagcccgga catctatacc cgttacttta tggcagcggg cgcgattggt
    27421 gcattgtata aaatgaacgc ctctatttcc ggtgcggaag ttggttgcca gggcgaagtg
    27481 ggtgttgcct gttcaatggc tgctgcgggt cttgcagaac tgctgggcgg tagcccggaa
    27541 caggtttgcg tggcggcgga aattggcatg gaacacaacc ttggtttaac ctgcgacccg
    27601 gttgcagggc aggttcaggt gccgtgcatt gagcgtaatg ccattgcatc tgtgaaggcg
    27661 attaacgccg cacggatggc tctgcgccgc accagtgcac cgcgcgtctc gctggataag
    27721 gtcatcgaaa cgatgtacga aaccggtaag gacatgaacg ccaaataccg cgaaacctca
    27781 cgcggtggtc tggcaatcaa agtccagtgt gactaatact tcttactcgc ccatctgcaa
    27841 cggatgggcg aatttatccc cgctttctcg tctgctgtaa tattccccac tacacttcca
    27901 ctgttgcgtc aggcgtttgt cgccatacgc ttacagggtg gcccgcatgc aaaaagcaca
    27961 acggatcatt aaaacctatc gccgtaatcg aatgattgtt tgtacgattt gcgcactcgt
    28021 tacgctcgct tcgaccctga gcgtgcgatt tatttcacag cgtaacttaa atcaacaacg
    28081 ggtagtacaa ttcgccaatc atgctgtaga ggaattagat aaagtactgc ttcccctaca
    28141 ggcaggtagc gaagtcttgc ttccgctgat tggtctgccc tgctctgtcg cccatttgcc
    28201 attacgtaaa caggcggcaa aactccaaac tgtgcgatcc attggcctgg tgcaagacgg
    28261 cacactctat tgctccagca tttttggtta tcgcaatgtg cccgtcgtgg acattctggc
    28321 tgaacttcct gcaccgcaac cacttttacg cctgacgatc gaccgtgccc tgattaaagg
    28381 cagtccggtt ttgattcaat ggacgcctgc agcgggcagt agcaatgctg gggtcatgga
    28441 gatgattaac atcgacttac tgacggcaat gctccttgag ccacaactgc cgcaaatcag
    28501 tagcgccagc ctgacggtgg acaaacggca tttgctctat ggtaatgggc tggtagattc
    28561 ccttccgcaa cctgaagaca atgaaaacta tcaggtttct tcgcaacgct ttccttttac
    28621 cattaacgtt aatggtccgg gggctacggc gctggcatgg cactatcttc caacacaatt
    28681 accgctggcg gtgctgctaa gtttactggt gggctacatc gcctggctgg cgaccgctta
    28741 ccggatgagc ttttcccgcg aaatcaatct gggcctggcg caacatgagt tcgaattgtt
    28801 ctgtcagcct ttgcttaatg cgcgcagcca gcaatgtatt ggtgtagaga ttttgctgcg
    28861 ctggaacaat ccgcgtcagg gctggatttc accggatgtg tttattccta tcgcggaaga
    28921 acatcattta attgtgccac tgacccgcta tgtgatggca gaaaccattc gtcagcgcca
    28981 tgttttcccg atgagtagtc agtttcatgt tggcattaac gtcgcaccca gccattttcg
    29041 ccgtggtgtg ctgataaaag atctcaatca gtactggttt agcgctcacc cgattcagca
    29101 actgatcctc gaaatcaccg aacgcgatgc cttactggat gttgattatc ggattgcccg
    29161 cgagctgcat cgtaaaaatg tcaaactggc gattgatgac ttcggcaccg gcaacagttc
    29221 attttcctgg cttgaaacat tacgtcctga cgtgctgaaa attgataagt catttaccgc
    29281 agctataggt tctgacgcgg ttaactcgac ggtgaccgat atcatcatcg cgctggggca
    29341 aagactgaat attgaactgg tggcggaggg cgtggaaacg caagaacaag cgaagtattt
    29401 gcgccgtcat ggcgtgcata ttttgcaagg gtatttgtac gcacagccga tgccgctacg
    29461 tgattttccc aaatggctgg cgggcagcca accgccgccc gcccggcata atggacatat
    29521 cacgcccatt atgccattac gttaacgctt actcatcttc atcgtgcgcc ggttgctctt
    29581 taacaatgcg aaccagatca acacgataat cattggcttc aatgatggtg atatgcagtg
    29641 gccctacatc aatcacatcc cccacacggg gaatgtgacc atttgccgag atcacgaggc
    29701 ccgcgaccgt cgcgatatca tcgtcatcgg caaggtgctc aacatcaagc gcctgctgca
    29761 aggcatgcaa atctgtaccg ccttttacca gccagccgtc accatcagta atgatttccg
    29821 gcgtttcgtc agcgtccggg aattcacccg caatggcttc cagcacatcc agcggcgtga
    29881 ccaagccttg taccacacca aactcgttgg tcacgataac aaagctcccg cgagcacgac
    29941 gcagcacgcc cagcaggttg atcggatcga gggtttcagg gacgataatc gccggagacg
    30001 ccgaagcaat cgccgccaca tcaacgccct cttccagcgc caccagcagt tctttagcac
    30061 gtacgattcc gatgatttca tccagttcac cgcgacatac cgggaacagg ctgtgcggtg
    30121 aggagagcag ctgctcgcgg atttcatcga ccccgagatt agcgtcaacc cagcttattt
    30181 caccgcgcgg cgtcatgatc ccgcgcagag aacgcgacgc cagcgtcagt acgccgttaa
    30241 tcatgtaacg ttcttcttcg gcaaatgcac cttccgggat cggcatcggc atcgggttat
    30301 cggcatcgtg ctgaacattg gcctgacgtt tcccgcccat caaacgcagg atggcatcgg
    30361 cagtacgcgc tcgcagcggc aaagtcgact ggtgacgaat aaagttgcga cgcgcaatct
    30421 ggttaaacac ttcgatgatg atcgagaagc caatcgcggc atacaggtaa cctttcggaa
    30481 tgtggaaacc gaaaccttct gccaccagac tcagaccaat cattaacagg aagctcagac
    30541 agagcaccac caccgtgggg tgctggttaa caaatcgcgt cagcggtttg gatgccagca
    30601 acataaccgc catcgcaatc actaccgccg ccatcatcac cggcagatgg ttaaccatcc
    30661 ctacagcagt aattaccgca tccaacgaga agacggcgtc aaggatgaca atctgtgtga
    30721 cgactaccca gaaactggcg tagcctttac cgtggccgga atcatgatcg cggttttcca
    30781 gccgttcatg cagttcggtt gttgctttga acagcaagaa tatccccccg aacaacataa
    30841 tcaggtcgcg tccggagaag gagaaatcca tgacggtaaa tagcggtttg gtcagcgtga
    30901 ccatccatga aatcagcgac agcagcccca gacgcataat cagcgccagt gataacccca
    30961 gcaaacgcgc tttatcgcgt tgttttggcg gcagtttgtc agcaagaatg gcgatgaaga
    31021 ccaggttatc gatacccagc acaatttcga gaacaacaag cgtgagtagc cccgcccaaa
    31081 ttgaggggtc cattaagaat tccatgacaa gctcctgctt aaggaatagc tattcgacgc
    31141 cagaaataat gcaggcgtaa cgacaaaatg caaacgaaag gtgcggcata gagtgccaga
    31201 aaggcaggcg ttaaaaggcc tgatgctgaa atgacgtcgg tgacgatcca tactgcgggc
    31261 tactgcccta tactccatgg ttgttaaacg ggagttaaac atatcagaga cgcctctgat
    31321 ttggcaaaga tttaccttcc tttgcaaacg aatgtgacaa ggatatttta cctttcgaaa
    31381 tttctgctaa tcgaaagtta aattacggat cttcatcaca taaaataatt ttttcgatat
    31441 ctaaaataaa tcgcgaaacg caggggtttt tggttgtagc ccttatctga atcgattcga
    31501 ttgtggacga cgattcaaaa atacatctgg cacgttgagg tgttaacgat aataaaggag
    31561 gtagcaagtg accattgcta ttgttatagg cacacatggt tgggctgcag agcagttgct
    31621 taaaacggca gaaatgctgt taggcgagca ggaaaacgtc ggctggatcg atttcgttcc
    31681 aggtgaaaat gccgaaacgc tgattgaaaa gtacaacgct cagttggcaa aactcgacac
    31741 cactaaaggc gtgctgtttc tcgttgatac atggggaggc agcccgttca atgctgccag
    31801 ccgcattgtc gtcgacaaag agcattatga agtcattgca ggcgttaaca ttccaatgct
    31861 cgtggaaacg ttaatggccc gtgatgatga cccaagcttt gatgaactgg ttgcgctggc
    31921 agtagaaaca ggccgtgaag gcgtgaaagc gctgaaagcc aaaccggttg aaaaagccgc
    31981 gccagcaccc gctgccgcag caccaaaagc ggctccaact ccggcaaaac caatggggcc
    32041 aaacgactac atggttattg gccttgcgcg tatcgacgac cgtctgattc acggtcaggt
    32101 cgccacccgc tggaccaaag aaaccaatgt ctcccgtatt attgttgtta gtgatgaagt
    32161 ggctgcggat accgttcgta agacactgct cacccaggtt gcacctccgg gcgtaacagc
    32221 acacgtagtt gatgttgcca aaatgattcg cgtctacaac aacccgaaat atgctggcga
    32281 acgtgtaatg ctgttattta ccaacccaac agatgtagag cgtctcgttg aaggcggcgt
    32341 aaaaatcacc tctgttaacg tcggtggtat ggcattccgt cagggtaaaa cccaggtgaa
    32401 taacgcggtt tcggttgatg aaaaagatat cgaggcgttc aaaaaactga atgcacgcgg
    32461 tattgagctg gaagtccgta aggtttccac cgatccgaaa ctgaaaatga tggatctgat
    32521 cagcaaaatc gataagtaac gtattgtgtt gattatcact cagttttcac actttagtct
    32581 tacgtaaaca ggagaagtac aatggagatt accactcttc aaattgtgct ggtatttatc
    32641 gtagcctgta tcgcaggtat gggatcaatc ctcgatgaat ttcagtttca ccgtccgcta
    32701 atcgcgtgta ccctggtggg tatcgttctt ggggatatga aaaccggtat tattatcggt
    32761 ggtacgctgg aaatgatcgc gctgggctgg atgaacatcg gtgctgcagt tgcgcctgac
    32821 gccgctctgg catctatcat ttctaccatt ctggttatcg caggtcatca gagcattggt
    32881 gcaggtattg cactggcaat ccctctggcc gctgcgggcc aggtactgac catcatcgtt
    32941 cgtactatta ccgttgcttt ccagcacgct gcggataagg ctgctgataa cggcaacctg
    33001 acagcgattt cctggatcca cgtttcttct ctgttcctgc aagcaatgcg tgtggctatt
    33061 ccggccgtca tcgttgcgct gtctgttggt accagcgaag tacagaacat gctgaatgcg
    33121 attccggaag tggtgaccaa tggtctgaat atcgccggtg gcatgatcgt ggtggttggt
    33181 tatgcgatgg ttatcaacat gatgcgtgct ggctacctga tgccgttctt ctacctcggc
    33241 ttcgtaaccg cagcattcac caactttaac ctggttgctc tgggtgtgat cggtactgtt
    33301 atggcagtgc tctacatcca acttagcccg aaatacaacc gcgtagccgg tgcgcctgct
    33361 caggctgctg gtaacaacga tctcgataac gaactggact aacaggtgag cgaaatggtt
    33421 gatacaactc aaactaccac cgagaaaaaa ctcactcaaa gtgatattcg tggcgtcttc
    33481 ctgcgttcta acctcttcca gggttcatgg aacttcgaac gtatgcaggc actgggtttc
    33541 tgcttctcta tggtaccggc aattcgtcgc ctctaccctg agaacaacga agctcgtaaa
    33601 caagctattc gccgtcacct ggagttcttt aacacccagc cgttcgtggc tgcgccgatt
    33661 ctcggcgtaa ccctggcgct ggaagaacag cgtgctaatg gcgcagagat cgatgacggt
    33721 gctatcaacg gtatcaaagt cggtttgatg gggccgctgg ctggtgtagg cgacccgatc
    33781 ttctggggaa ccgtacgtcc ggtatttgca gcactgggtg ccggtatcgc gatgagcggc
    33841 agcctgttag gtccgctgct gttcttcatc ctgtttaacc tggtgcgtct ggcaacccgt
    33901 tactacggcg tagcgtatgg ttactccaaa ggtatcgata tcgttaaaga tatgggtggt
    33961 ggcttcctgc aaaaactgac ggaaggggcg tctatcctcg gcctgtttgt catgggggca
    34021 ttggttaaca agtggacaca tgtcaacatc ccgctggttg tctctcgcat tactgaccag
    34081 acgggcaaag aacacgttac tactgtccag actattctgg accagttaat gccaggcctg
    34141 gtaccactgc tgctgacctt tgcttgtatg tggctactgc gcaaaaaagt taacccgctg
    34201 tggatcatcg ttggcttctt cgtcatcggt atcgctggtt acgcttgcgg cctgctggga
    34261 ctgtaagact gttgtacact accggggcct tttggccccg tttttttatc tggaggatta
    34321 atgacaatca cggacctggt actgattctt ttcatcgccg cactcctggc cttcgcgatc
    34381 tacgatcagt tcatcatgcc ccgccgtaac ggccccaccc tgctggcaat tcctttgctc
    34441 cggcgtggtc gcatcgatag cgttatcttc gtcggattga ttgtcattct tatctataac
    34501 aacgtcacga atcatggtgc gttaataacg acatggttat taagcgcact ggctctgatg
    34561 ggtttttata tattctggat ccgcgttccg aagatcatct ttaaacaaaa aggttttttc
    34621 ttcgccaatg tctggattga atatagccga atcaaagcga tgaacttgtc ggaagatggc
    34681 gtgttggtga tgcaattaga acagcgtcgg ctgttaatcc gcgttcgaaa tatcgacgat
    34741 ctggaaaaaa tttataagct tctcgtatca actcaataac ttatgaattt agccaaagct
    34801 atgtttagtg tatttttaat aatcagacat agcttaggct atattatctc ttcccttatt
    34861 tgttatttat attaacgttt cattgatata taaatctaaa tgaaaatcgt tatcaataaa
    34921 gcaatgaaat aatatattcc aacagttgtt ttatattctc aaaatatgtt aaggttgcgc
    34981 cctcatttgg ggagtagccg atttccagat tccggaaatg tacgtgtcaa catactcgtt
    35041 gcaaaacgtg gcacgtacgg actgaagact ttcagtcagg cgagaccata tgcacatcaa
    35101 tcgctatgcc tgcatgaacg cgaaaaccca tcgcggcttg ttgttcatgt atgcatgttt
    35161 gctgggggcg atgatgtgtt ttatggatac cccggtcagg acgttgtcat gaatatcact
    35221 gctactgttc ttcttgcgtt tggtatgtcg atggatgcat ttgctgcatc aatcggtaaa
    35281 ggtgccaccc tccataaacc gaaattttct gaagcattgc gaaccggcct tatttttggt
    35341 gccgtcgaaa ccctgacgcc gctgatcggc tggggaatgg gcatgttagc cagccggttt
    35401 gtccttgaat ggaatcactg gattgcgttt gtgctgctga tattcctcgg cgggcgaatg
    35461 attattgagg gttttcgtgg cgcagatgat gaagatgaag agccgcgccg tcggcacggt
    35521 ttctggctac tggtaaccac cgcgattgcc accagcctgg atgccatggc tgtgggtgtt
    35581 ggtcttgctt tccttcaggt caacattatc gcgaccgcat tggccattgg ttgtgcaacc
    35641 ttgattatgt cgacattagg gatgatggtt ggtcgcttta tcggctcaat tattgggaaa
    35701 aaagcggaaa ttctcggcgg gctggtgctg atcggcatcg gcgtccagat cctctggacg
    35761 cacttccacg gttaataaga acgctgccag aggtgaatat taaagtccgt ctggcagtcg
    35821 aacacttctt ttgctgccag cgtttgccag acttctggct tcgcacgcca ggcaaacggc
    35881 gtcatctgca ataatgcgac ggcttcatca ccgcgaagac gcatcggata acacaactca
    35941 tcactctgct gtaatgtaaa accttccagt tgttctgcat gaggtgcatg aagatgtact
    36001 tcattgtaaa tcagcccctt cagctccatc aaatgtcgcg gtcccggcgt ggcagtaatg
    36061 acccaaccgc cgggcttcac tactcgcgct aattcttctg ctttacacgg cgcgtaaata
    36121 cgtattatgg cgtccatact ggtatcggaa aacggcaaac ggtggctgga agcgacacaa
    36181 aaagtgacct gcggatagcg tttcgccgcc gcttttatcg ctaccttcga gacatccagg
    36241 ccaaaggtgg tgatttcggg caacgcatcg gcaaatgcgt gtgtgtaata cccttcacca
    36301 cagccaatat ccagcaccgc cgtggcctta tcatcaagcc gttcccgcag ttgggcgaca
    36361 attgcatcac gcagcggctg ataatgtccg gcatctaaga atgcgcggcg tgcttgcatc
    36421 atttccgcgc tgtcgcccgg atcacgagac cgtttatgct gaacgggcag cagattgaca
    36481 tacccttctt tcgccatatc aaactgatgt cgttggggac agatatagct gtttttttca
    36541 cgcgtaagag gctgatggca aagtggacag gaaaacgaca tgacaactcc ggcaggatac
    36601 taaaggccgc aagtgtaacg cgaatcacgc ggcgggcaaa tatccgaacg ccaggagcga
    36661 agataaagca tagtaaaaag cctcgcattc gacgaggctt tatagcgaga ttgaagcgta
    36721 ttcacacttc agatcagtgg attcgatcag atagctgtta cgttaacagc tgccggacct
    36781 ttctggccgt cctgaatttc gaactcaacg ttctgacctt cagccagagt tttgaagcca
    36841 ttaccctgga tagcggagaa gtgtacgaac acgtctttgc tgccatcagc aggagtaatg
    36901 aagccaaaac ctttagactc gttgaaccac ttaacctgac ctttaatctt tgccatttga
    36961 aaaattcctt agattgtttt cttcgcccgc aggcataaca tagataaaac tgacacatta
    37021 ctgcatgagg caccaatata aggctcggca gagaagcggt attcaacgtc aacgtgttta
    37081 ctcaggactt ctttactgaa aatgccacac ataaacagaa ctgtacctcg tttaacccga
    37141 aatctgttat cacatacaac gttaattatg gcaagccatt tttaaacatg tctcgatcag
    37201 acgcacaaat ctcgatacgt cttccacttt ttttgcacac ttatgcaacg gaatacgcgc
    37261 cgatatatca ttgtgcttaa ccttgccagt tcaggcagat acttaaactg gcgtagtttc
    37321 taacatagtt caataagttc agttgttcca ggccagtggg agaagttatt acatagtgcg
    37381 tgcaatatca cattttctgc tatgcaatga ataaaaagtt atatcacttt ttctcataaa
    37441 acagtcagtt aacggctatc aattacccta aagagaagtc aatccccaaa aggattgtaa
    37501 atttaaaata agaaaaattg atgagtgagc aaaaaaaatc aagagagaaa cgtttcgcta
    37561 ataaataata ccacgcttaa tcacaaatcc gatgtaaaca tcctaataaa ttaatgagga
    37621 aaatatcttt ttctgatatc ccgttacgca aataaataat atgataatta tttgaagtca
    37681 gaccagagtt ttaatttgaa gcagttgcac catgacagtg cgtataatca ggatgaattg
    37741 agtaaccctt caacgacggg gttactcaag attgaggaag gattatcgtt cggcgaccag
    37801 gcgaataata tcttcgtctt caaccgattt ttgactctca atggtctttt tatctgcttt
    37861 ctctggttcg tttgcttcgc acaaacgacg taacagtgca ttttgacgtt tttgctgatc
    37921 cagcaacgcc tcaagcagtt caatctgttc gttagtccga gaactggcac gattaataaa
    37981 aaaccacaag atgagcccga taagaagaac caccaccgat acaaccaaag acgcaatgtt
    38041 catcacgcct gaatttacaa cttcgttcat ttcaccacct ctgagtagag gcgctattct
    38101 accactgctg gagaggaaga aaatctagtg ctgaaaaaat gatatcacca cgggataaac
    38161 tggttaatgg cacaaattcc gctgaaaaat tgtacatcct gatcgcacat catgttgaat
    38221 acctgcgccc aaagcagcaa gcaagccaac accacgacaa ccagaacgac ccatcgaaac
    38281 tttttcactc caatctccgt ttcacctacc ttatgtcatt aaactagcat gcgttagtta
    38341 aacagcgcct aactatgtcg atattcgtgc tttttcggaa tgagtcgctt gcttcagcgc
    38401 atattgccgc tacgattaag cgaacataaa aagagaagag gttgtaatgc gattcatcat
    38461 tcgcacagtt atgctgatag cactggtatg gataggatta ttactcagcg gatatggcgt
    38521 tttgattgga agcaaagaga atgccgcagg attaggtttg caatgtacct atctgactgc
    38581 cagaggcacc agcacggtac aatacttgca cactaaaagt ggttttttgg ggataacaga
    38641 ttgcccgctg ttacgcaaaa gcaatatcgt ggtcgataat ggttaagtgt aataaataac
    38701 cagattctca ctcatgagta aaatattctt atagcaactg cgtttaatct aacgccacct
    38761 tcaactttat ttttctcatg ctaaataaag aacctgtctg tttgatattc cgacaggttc
    38821 ttactctcat cagaacggat agtcgtgata acccatttgg gcagaaattt tgcgcgctgc
    38881 ggtatgcaac attgcgacat attcctgtaa acgctcttca gagaaacgca acgtcgggaa
    38941 ggagatgctc aaaccggcaa tgaccacgcc aaagcgatca aataccggaa ccgcaatgca
    39001 tcgcagccct tcttcctgct cttcgttatc ttcgccatac ccctgctcgc gcacctggtc
    39061 cagaaccggt aataacgctt ctgtactggt gatggtccgc tcggtactgc gtttatactc
    39121 cacgccctca agaatttgct tcacttcatc gcgatcgcgc catgccagca gtaccttacc
    39181 aatcgcggtg ctgtacagtg gattacgacg cccaatccgt gaatacatgc gcaaattgta
    39241 catagagtca attttgtgaa tgtaaacaat actgtcttcg tccagtgcgc cgaggtggat
    39301 agtttctttg gtcaggcggg agatctcacg catctggata tctgcgctac gaattaaatc
    39361 gacgttttgt aacgcgcgag cgcccagttc aaacaatttc agggtcaggg aatatttctc
    39421 cgactcccct tcctgcgcca cataacctaa ggttttcatg gtctgtaaaa agcgataaac
    39481 ggtgcttttt gacatcatga cgcgctgcga cagctcggtt atccctattt cgcgctcttc
    39541 acccagcgcc tgcaaaatgc caaaaacttt tagcacggaa gatacagaat caggctgttt
    39601 atccagatct gcgttagcca tttatcacct cattgcgagt gttttataaa aatcagaact
    39661 gttttttatt ataatttcgc accagggtgg tcgcaatcca tcttttgccg gttagttaca
    39721 attctgcgac atccaccgtg aatatcagtg ctagaatcat acccctgttg attattcacc
    39781 aaagatttaa aattcctatg ccaaaagttc aggccgacgg cctgccattg ccccagcgat
    39841 acggtgcgat attaaccatt gtgattggta tttcgatggc tgaccttgac ggcgcaatcg
    39901 ccaacgtcgc cctgccaaca atcgccacgg accttcatgc cacgccagcc agttccatct
    39961 gggtagtgaa cgcctatcaa atcgccattg tcatctccct gctctcgttt tcgtttctgg
    40021 gtgatatgtt tggctatcga cgtatttata aatgcggtct ggtcgttttt ctgttgtctt
    40081 cactgttctg cgccctttct gattcgctgc aaatactcac ccttgcgcgt gtcatacaag
    40141 gtttcggcgg tgcagcgttg atgagcgtta ataccgccct tatccgcctg atctatccac
    40201 aacgttttct gggtagaggg atgggcataa actcgtttat tgttgccgtc tcttctgctg
    40261 ccgggccgac aattgctgca gcaatcctct ccatcgcatc ctggaaatgg ttatttttaa
    40321 tcaacgtacc gttaggtatt atcgccctgc ttctggcgat gcgttttctg ccgcccaatg
    40381 gttctcgcgc cagtaaaccc cgtttcgacc tgcccagcgc cgtgatgtac gcgttaacct
    40441 tcggcctgct tatcactgcg ttgagtggtt tcgctcaggg gcaatcgctg acgttaattg
    40501 ctgcggaact ggtggtaatg gttgttgttg gtattttctt tattcgccgc cagctttctc
    40561 ttcccgtacc gctgctaccg gtggatttac tgcgtatccc gctgttttca ctttctattt
    40621 gcacatctgt ttgctctttc tgcgcacaaa tgctggcaat ggtttccctg cccttttacc
    40681 tgcaaaccgt cctcgggcgt agtgaagtcg aaacaggttt acttctgaca ccgtggccgt
    40741 tagcaacgat ggtgatggct ccgttggcag gctatttgat tgaacgcgta catgcaggat
    40801 tgctgggtgc tttaggatta ttcatcatgg ctgcggggct tttttccctg gttctgctgc
    40861 ccgcgtcacc tgcggatatc aatattatct ggccgatgat cttatgtggc gctggatttg
    40921 gcttgttcca gtcacccaat aaccacacca ttattacctc cgctcctcgc gaacgtagcg
    40981 gtggagccag tggcatgtta ggaacggctc gtctactggg tcagagtagc ggcgcggcgc
    41041 tggtggcgct gatgctaaat cagtttggag ataatggtac acacgtctcg ctgatggctg
    41101 cggctattct ggcagtgatt gctgcctgtg tcagtggttt acgtatcact cagccacgat
    41161 ccagggcata aaaaaaagcg cgtcgatcag gacgcgcttt ttagtggtta cttcataata
    41221 ttacttcagg tattcacccg tacgcagagc ttcaattcgt ttatccagcg gcgggtgagt
    41281 catgaacaac tcactgagcg atttcgactt accgttaatg cagaaagcca tcatgctggt
    41341 tgcttcttgc ggttcatagc tggttttcag gcgctgcagc gcggcaatca ttttctcgcg
    41401 accaaccagt tttgccgaac cggcatcagc atggaattca cgatgacgcg agaaccacat
    41461 ggtgataatg ctcgccagaa taccaaacac cagttccaga accgttgcaa ccgcaaagta
    41521 gatcagcggg ttgccgttgc tctcttcacc ttcatcacga tttccgccca taaaacccgc
    41581 ggcaagctgc gccaggatac gggaaataaa gataacgaag gtgttcacca cgccctgaat
    41641 cagcgtcatg gtgaccatat caccattggc gatgtggctg atttcgtgag caattaccgc
    41701 ctcggcttca tccgggctca tgttctgcag caaaccggtg ctgacagcaa ccagagaggc
    41761 atcacggcgc gcaccggttg caaaagcgtt gatgtccggc gcatggtaga tagccacttg
    41821 cggcatagcg atccccgcct gacgagcctg ggttgctaca gtattgacca gccaacgttc
    41881 cctttcgtta cgcggttgct cgatcacttc cccgccaaca gatcgtaatg ccatccattt
    41941 ggacatcaga agcgaaacga aggaaccacc aaaaccgaac agcaaggcca tgatcatcag
    42001 cccctgaacg ctgctcgact gtatccctgt caggctcagt accagcccga aaacgaccat
    42061 tacggccagg ttcgttagca ggaagagcgc gattcgcatc ataattttct ttttacctca
    42121 gtttaacaaa acgcaatatg cgatacccac atcgtatggg ttacgcgact attttcaagt
    42181 ctggatagtg cgtaagtcac cagaaagaca caactttaca ttttgtagca tctgatttac
    42241 ggcatcttgc cgctgttaaa aaatcaggca caatttcttg tgcctgattg atattacttg
    42301 acgggtgcgg gttgttccgc gggtctggct ttttcaagct tcgccagatc gagtgcgata
    42361 ttcaccgtct catccagata aggatccggc tcctggtaat ctttcggtag atcatccagt
    42421 ttcttcaact ccggtttacc ttcgcgttta aagcgttcgt tcaaacgcgc cagacgcgtc
    42481 gcatcatctt cattattctc tttctcacgc acagcgtaat tcagagaaac gatattgcgc
    42541 ttgtccttca tggcgttgaa gcgcgcgata tccttcatga tgttctggaa ctcaggatct
    42601 ttcgcgatac gcgcattatg ttccttcagc agctccggtt caaaggccgt taaatcgcct
    42661 gatttcacat aggtcgcggc atcaatgcta tcccacggca gcgcgttatc ttcgaatttc
    42721 tcacccgttt ccgtttcttc attacccgtc ggcatgatga tgtctggcgt tacgccttta
    42781 cgttgcgtac tgccgccgtt aacgcgatag aatttctgga tcgtgtactg cacagaaccc
    42841 agcgctggcc attcaggacg taacatctga tcgtaaatac ggttcaatga acggtattgc
    42901 tgaacggtgc ctttaccaaa cgtcggttca cccacaacca gcgcacgacc gtaatcctgc
    42961 attgccgcgg caaagatttc tgaagccgaa gcactgaagc ggtcaaccag taccaccagc
    43021 gggcctttat agaaaacctg tccgtcggta tcgctgtctt cacgaacttt accgttgtta
    43081 tcacggacct gaacaatagg acccgcagga ataaacagac ctgagagcga taccgcttcg
    43141 gtcagcgccc caccaccatt gctacgcagg tcgatgatca cactgctgac attctgtttt
    43201 tccagtttct gcagttgtac tttgacatcg tctgtcaaac ccacatagaa gcccggaata
    43261 tccagtacgc cgactttctc tttaccgacg gtcttcaccg acattttaac cgcgcggtct
    43321 tcgagacgaa tacgttcacg ggtcaatgtt acagtgcggg tcttggtccc tttaccagca
    43381 ggtaaaattt ccagacgaac tttactgccc ttcggccctt taattaaggc aaccacatca
    43441 tcaagacgcc agccaatcac gtcaaccatc ggcttgcctg tttgaccaac accgacaatt
    43501 ttgtcaccaa cgctgatagc tttactcttc gctgccggac cacctgccac catcgaattg
    43561 ataacggtgt agtcatcatc catttgcagc actgcgccaa taccttccag cgacaaactc
    43621 atttcagtgt tgaactgttc ggtattacgc ggggaaagat agttggtatg cgggtcgatt
    43681 tcacgcgcaa acgccgtcat tgccagcgag aaaacatctt cgctgttggt ttgcgccaga
    43741 cgacgaatgg caaatttgta gcgtcgagtc agggtttcac gaatttcttt atccgttttt
    43801 cctgccagct tcaggcttaa ctcgtcgaat ttgactttac tgtcccacag cgcgttcaac
    43861 tcagcctcgt ttttcggcca gggcgctttg ctgcggtcaa ggttataagt gtcgttgccg
    43921 gtgaaatcca tcggcttttc cagtaccgac aaagcgtact ggtaacgttc aaaacgacgc
    43981 ttttgcgcca gattgtagag atcgtagaaa acgtcgagtt tgcctgaacg cagttcatcg
    44041 cctaactcgg ttttcttttt cgcgaactgt tcaacatcgc ttgccagcag cacgttgtgg
    44101 ctgtaatcga gcagattcag gtagcggtca aagattttgg ccgaaaatgc ctgatcgagg
    44161 tcgaactggc gataatgaga acgggtgaag cgcgacgtta cgcgctcact caccgtcgca
    44221 tgctgcgtct cttcctttaa taccggaatt tgatcagcac gcgtgatatc ttctacagcg
    44281 aaggtctggc ctgctattgc aagcaggcca gctaacgcgg taagcctaaa aaacatgttc
    44341 atgcctggcc cggcctccgt ttcagaacac caggtgttct gcgcgcacaa tcaaagacat
    44401 acccgaattc agctggacgc ggacgccgtc tttggtgatt tctaatacgg tggcatccat
    44461 cgcgttttga cccgctttca ccttcagggc ttgtccgaca gtcagagctg aaatgtcaga
    44521 aactggggtg tgctgttctt cgcgaggtgc ttttgctgtt tttggcgctt tctctaccgg
    44581 cttttgcgca cgaggtttac gttcagcgcc ttctttgcgg cgtggcgtgg tcggacgtgg
    44641 cttgcgttcg cgacgcggtg cgtcttcttt ctcaccagca gctgcggcag cttcgcgttt
    44701 tttcgcttgc tgttcagcac gctgtgcctg aacacgcgct ttcgcttctt caagctgctt
    44761 gcgagcatgc tctacatgtt gctcgtccag ctcaccgcat gggttgccgt caagatcgac
    44821 acgcgttgcg ccgggtttaa caccgtaaag gtaacgccag ctcgaagtgt agagacgtaa
    44881 agcggatcgt aattgcgttt tgctcaggtt catttccccg gcaacacgat cgaccaaatc
    44941 ctgaaaaata ccgattttca gcggacgcgc ttcaccttcc gcactgaaac agtggggaaa
    45001 acgttcggcc agaaacgcga ttacttcttt actgctattc aacttaggtt gattttccat
    45061 gaaatttcct gattacaacg gacgtagcca acaagcgcag gcatgaacag gcgtcattat
    45121 aatgacgcta tcagtaaatg ctacgttatc cgttgattat cctgcgacgc tcgcaaagaa
    45181 ttttttgtaa tccgtcgttg caagcacctt ttcaagctgt gccacaagct gacgtaagcc
    45241 ttgctcgtcc tcgtctgtaa agcgaccgaa gacggtacta tcgatgtcga gaacaccaat
    45301 aatctgattt ttcaccacca gcggcagaac aatttcagaa ttactcgccg catcacaggc
    45361 aatatgcccg tcaaacgcat gaacatcctc gatacgctgc acttgattgc gggcaaccgc
    45421 agtgccgcac acgccacgcc cgacgggtat ccggacacag gcaattttgc cctgaaatgg
    45481 tccgagcacc agtgtatcgt cctcaagcaa ataaaaacct gcccagttta cgtcagtgag
    45541 acgctcatat aacaacgcac tggtgttcgc aagcgttgcc agaaaactgg tttctcccgc
    45601 catcagcgcg ttaaagtcgc gatttaaatc cgcgtaaaat tctgttttgt tcattatata
    45661 atcacttggt tgtcttacct ggaactgcca gcctattaaa ataagcatta aatgcgttaa
    45721 tgctcaagat cattcccatc atgggttaag attaatgtta attcttatta catttggcac
    45781 gtcatggctc ttaacacacc acaaattacg ccgacaaaaa agataacagt gagggcaatc
    45841 ggcgaggaac tgccgcgtgg tgattaccaa cgttgcccgc aatgtgacat gctgtttagc
    45901 ctgcccgaga taaattctca tcaaagtgcc tattgtccgc gctgtcaggc aaaaattcgt
    45961 gacgggcgcg actggtcgct aacgcgcctg gcggcaatgg ccttcaccat gctgttgctg
    46021 atgccgtttg cctggggcga accgctgttg catatctggc tgttaggtat tcgtatcgac
    46081 gccaatgtca tgcaaggcat ctggcaaatg accaaacagg gcgatacgat aacgggggcg
    46141 atggtctttt tctgcgttat tggtgccccc ctcattctgg tgtcctccat agcatattta
    46201 tggtttggga accgactggg aatgaattta cgtccggtac tgctgatgct tgagcgactt
    46261 aaagagtggg taatgctcga tatctacctg gtcggcattg gcgttgcttc tataaaggta
    46321 caggattatg cccatatcca ggcgggtgtc ggcttgttct cttttgtggc gttggtgatt
    46381 ttaacgacgg tgacgttgtc acatcttaat gtcgaggagc tgtgggagcg attttatccg
    46441 cagcgccccg ctacgcgtag ggacgagaaa cttcgtgtct gtcttgggtg ccattttacc
    46501 ggctatcctg atcagcgtgg tcgctgcccg cgttgtcata ttccgctacg cctgcgtcgc
    46561 cgtcatagtt tgcaaaaatg ctgggcggcg ctgttagcgt caatcgtttt gttgttacct
    46621 gccaacctgt tgcctatttc tatcatttat ctgaatggtg gccggcagga agatacgatt
    46681 ctttccggga ttatgtcgct ggcaagtagc aacattgccg ttgcgggaat cgtgtttatc
    46741 gccagtattc tggtaccgtt tactaaagtg atcgtcatgt tcactttact gttgagcatt
    46801 cattttaaat gccagcaagg tttacgcaca cgcattctgt tactgcgaat ggtgacctgg
    46861 attggtcgct ggtcgatgct cgacctgttt gtcatatctt taaccatgtc gctgattaat
    46921 cgcgatcaga tcctcgcttt tactatggga ccggctgcgt tttatttcgg cgcagcggta
    46981 attttgacta ttcttgctgt ggaatggctg gacagccgct tactttggga tgcacatgag
    47041 tcaggaaacg cccgcttcga cgactgaagc gcagattaaa aataaacgcc gtatctcacc
    47101 tttctggctg ctgccgttca tcgcgctaat gattgccggt tggctgattt gggacagtta
    47161 tcaggaccgg ggtaataccg tcaccatcga ctttatgtcg gcggatggta ttgttccagg
    47221 ccgtacgcct gttcgttatc agggcgttga agtcggaaca gtgcaggata tcagcctcag
    47281 cgacgatctt cgtaagattg aagtcaaggt cagcatcaag tccgatatga aagatgcact
    47341 gcgcgaagag actcagttct ggctggtgac gccaaaagca tcgttggcag gtgtctccgg
    47401 gctggacgcc ctcgtcggtg ggaactatat cggcatgatg ccgggtaaag gtaaagagca
    47461 ggatcacttt gtcgcgctcg atacccaacc gaaatatcgg ctggacaatg gcgatctgat
    47521 gatccacctg caagcccccg atctcggttc gctgaacagc ggttcattgg tctatttccg
    47581 caagatcccg gtgggaaaag tctacgacta tgccatcaat cccaataaac aaggcgtggt
    47641 gattgatgtc ctgatcgagc ggcgttttac tgacctggtg aaaaaaggta gccgtttctg
    47701 gaatgtttct ggcgttgatg ccaacgtcag tatcagtggc gcgaaggtga aactggaaag
    47761 tctggcggca ctggttaacg gtgcgattgc cttcgattca cctgaagagt cgaaacctgc
    47821 cgaggcggaa gatacctttg gtctgtatga agatctggcc cacagccagc gtggcgtaat
    47881 aataaaactg gaactgccga gtggggccgg attaaccgcc gactcgacgc cattaatgta
    47941 tcagggactg gaagtcggac agctaactaa actggattta aatcctggtg gtaaagtcac
    48001 tggggaaatg accgttgatc ccagcgtcgt taccctgctt cgtgaaaata cccgcatcga
    48061 attacgtaat ccgaaattat cccttagcga tgccaatctc agcgccctgc tgactggcaa
    48121 aaccttcgag ttggtacccg gcgatggcga gccacgcaaa gagttcgttg ttgtgccagg
    48181 cgaaaaagca ctgctgcatg aacctgatgt tctgacgctg accctgaccg cgccggaaag
    48241 ttacggtatt gatgcgggtc agccgctcat tcttcacggc gtgcaggtag gccaggttat
    48301 cgatcgtaaa ctcaccagca aaggcgtcac ctttaccgtc gccatcgagc ctcagcatcg
    48361 agaactggta aaaggcgata gcaaatttgt cgtcaacagc cgtgtcgacg tgaaggtggg
    48421 gctggatggc gttgagtttc tcggtgccag cgcctcagaa tggattaacg gcgggatacg
    48481 tattctgccg ggcgataaag gcgagatgaa agccagctat ccactgtatg ccaatctgga
    48541 aaaagcgctg gagaacagcc ttagcgattt acccaccaca accgtgagtt tgagtgcaga
    48601 gacgctgccg gatgtgcagg caggatcggt agtgctgtac cgtaaatttg aagttggtga
    48661 agttattacc gtgcgtccgc gagctaacgc gtttgatatc gatctgcata ttaagccgga
    48721 gtatcgcaac cttctgacca gcaatagcgt gttctgggca gaaggcgggg cgaaagttca
    48781 gctgaatggt agtggcctga ccgtacaggc atccccgctc tccagagcat taaagggagc
    48841 cattagcttc gataacctca gcggtgccag cgccagtcag cgtaaaggcg ataagcgtat
    48901 tctgtatgct tccgaaacag cggcccgtgc ggttggcggg cagattacgc ttcacgcttt
    48961 cgatgccgga aaactggcgg tcgggatgcc aattcgctat ctcggtattg atatcgggca
    49021 aatccagaca ctggatctga ttaccgcgcg taatgaagta caggcaaagg cggtgctcta
    49081 tccggaatat gtccagactt ttgctcgcgg aggtacgcgc ttctcagtgg tcacaccgca
    49141 aatttcggca gctggcgttg agcatctaga tactatcctc cagccgtata tcaacgtcga
    49201 accaggccgt ggcaatcctc gtcgcgactt tgaattacaa gaggccacca ttactgattc
    49261 gcgttacctg gatggtttaa gcattattgt tgaagcgccg gaagccggtt cgttaggcat
    49321 cggtacgcct gtgctgttcc gtggtctgga agtaggtacg gttaccggaa tgacgctggg
    49381 gacattgtca gatcgcgtga tgattgcgat gcgcatcagt aaacgctatc aacacctggt
    49441 gcgtaacaat tccgtcttct ggttggcatc gggttacagt ctggattttg gtctgacggg
    49501 cggcgtagtg aaaaccggca cctttaacca gtttatccgt ggcggcatcg ccttcgccac
    49561 gcctccgggt acgccactgg caccgaaagc ccaggaaggc aagcacttcc tgttgcagga
    49621 aagtgaaccg aaagagtggc gtgaatgggg aactgcgctt cccaaataat gcccactgct
    49681 ccggcgtgcc tgcgccggag cgtttatgct aaactgcgcg cctgtttttt tgccagtggt
    49741 atatgctcgt ggcccaacac accgtttatt tcccggacgc ctttctgacg caaatgcgcg
    49801 aagcgatgcc ttcgacgctc tcttttgatg attttcttgc cgcctgtcag cgcccgttgc
    49861 gccgcagcat tcgcgttaat acgctgaaaa tctccgttgc tgatttcctg caattaaccg
    49921 ctccttatgg ctggacgctt acgccaattc cgtggtgtga agaaggtttc tggattgaac
    49981 gcgacaatga agatgcattg ccattgggta gtaccgccga gcatttaagc ggcctgtttt
    50041 atattcagga agccagttca atgttgcccg ttgccgcctt gtttgctgac gataatgcac
    50101 cacagcgggt gatggatgtc gcagctgcgc caggctccaa aacgacgcaa attgccgcgc
    50161 ggatgaataa cgaaggggca atccttgcca atgagttttc cgccagtcgg gtaaaagtgt
    50221 tacatgccaa tatcagccgc tgtggtatca gtaatgttgc gctcacacat tttgatggcc
    50281 gcgtgtttgg tgcggcattg ccagaaatgt tcgatgccat tttgctggac gctccctgct
    50341 ccggcgaagg cgtggtgcgt aaagatcccg atgcgctaaa aaactggtca ccagaaagca
    50401 atcaggaaat cgcagctacc caacgggaac tgatcgacag tgcctttcat gcattacgcc
    50461 ctggcggtac actggtttac tcgacctgta ccttaaaccg ggaagaaaac gaatccgttt
    50521 gcctgtggct taaagagact taccccgacg cagtagagtt tttaccactt ggcgatctct
    50581 tccctggtgc aaacaaagcg ctgaccgaag aaggcttttt gcatgttttc ccacaaattt
    50641 acgactgcga aggcttcttc gttgctcgtc tgcgtaaaac tcaggccatt cccgccttac
    50701 ccaccccaaa atacaaagtg ggcaatttcc cgttcagccc ggtgaaagat cgcgaagccg
    50761 cacaaattcg tcaggcggct gcaagtgttg gcttaaactg ggatggaaac ctgcgcccct
    50821 ggcagcgtga caaagaactg tggttgttcc cggtgggcat tgaagccctg atcggtaaag
    50881 tccgattttc tcgcttgggg attaaacttg ccgagacgca taacaaaggt tatcgctggc
    50941 agcatgaagc ggttattgcc cttgcctccc ccgacaacga gaacgctttt gaactgacac
    51001 cgcaagaagc ggaggagtgg tatcgcgggc gcgatgttta cccgcaagcc gcgccagtgg
    51061 cggatgacgt gttggttact ttccagcatc agccgattgg tttagccaaa cggattggtt
    51121 cgcgactgaa aaacagctac ccgcgtgaac tggtgcggga cgggaaactt tttaccagta
    51181 acgcatgaca gcgcacaaaa aaagcgcact ttttgactgg cacattcggc ggcctcaact
    51241 aggctgaaaa atggtgcgat cggactggtc gtaccacaat cggcagctaa atggagagca
    51301 cgaagatgaa aacgagtgtg cgcataggcg cttttgaaat cgacgacggc gaattacacg
    51361 gcgaatcgcc gggtgatcga acgttaacca ttccttgtaa atctgacccc gatttatgta
    51421 tgcaactgga tgcctgggat gctgaaacca gtatccctgc cctgctaaat ggcgaacact
    51481 ctgtccttta ccgtacccgt tacgatcaac aatctgatgc ctggattatg cgtcttgcct
    51541 gatccaaaaa gaacccgtcg gcatggcggg ttatttgtcc tggttattcc cccgttgtaa
    51601 aatctctcct aaacttaacg gtacggcacc acacttcggg gatgaaatgt tcgcgctggt
    51661 actttttgtt tgctacctgg atggcggttg tgaagatatt gttgtggatg tctacaacac
    51721 ggaacagcag tgtctttatt ctatgagcga tcaacggatc cgccatggcg gttgttttcc
    51781 gattgaggat tttatagatg gtttctggcg accagcacag gagtatggtg atttttaatt
    51841 attgcaattg cacgaaagtc agttcgcccc caaagacagc gccggtatca atataatgca
    51901 ggttgccaat atccacgcga tggcgcaacg gtgtatgacc aaaccagaaa tgatcagcac
    51961 ctgtaattcc ctgccctttt tggcgttcac ttaatcgcga gcggctccac aagacctgat
    52021 gcaaatcaac gtccttttgc cattcataaa catcatctgg ataatcggca tgagcaataa
    52081 catgtttgcc ggtgcggctg tgtacttcaa gaataaaggg caaatgctga catttttcca
    52141 gcgccgtttt cgcttgtttc tgttgattat ctgccagcgc aataaaccag tcgccgccat
    52201 tcatcaacca caaagacatc tgctgggatg ccagcgcatc catcgccatc tgttcatgat
    52261 tgcctcttac cgcacgaacc caatgttgtt ccagtaactg cagacaacgt aaactttgcg
    52321 gcccacgatc gataacgtct cccactgaga taagtaaatc tcgccacgga tcaaaacgac
    52381 aatgccataa tttgcggcgc aactgctcaa gacaaccgtg tatatcgcca gaaagccaga
    52441 tatgtcgcca ttgatgaccc gcaattctct gataaacggg cgcaggctgt ttcatcaata
    52501 ttttcctccc gcgctaaaga tcacataatc ttaacaagaa tgttaaaaaa cgctggactc
    52561 agacagtaga gtgtgtgtta tggttgacta taaagtcagc gaaggaaatg cttctggctt
    52621 ttaacagata aaaagagacc gaacacgatt cctgtattcg gtccagggaa atggctcttg
    52681 ggagagagcc gtgcgctaaa agttggcatt aatgcaggct tagttgcctt gccctttaag
    52741 aatagatgac gacgccaggt tttccagttt gcgtgcaaaa tggtcaataa aaagcgcggt
    52801 ggtcatcagc ttaaatgtta aaaaccgccc gttctggtga aagaactgag gcggtttttt
    52861 tattggaaat caaaaggcta ttttaggtaa ttaacagagt ttttcagccc gttctataaa
    52921 cggtgccaga ctcatttttt cgccgggatt gttgggatca tcaatctgaa tcaccgaaat
    52981 gggttgggct ttagtcttcc cactggcaac ttccttctgt gcgatatcgt ttaaaggata
    53041 ctgcacgagg gtacttgggt taatgacata caaagcatta cccggtctgc aagtcagcat
    53101 cacctcttcg cgattaaacg cccatttgtc tttacccact tcaaaacggc tgacggtaat
    53161 cacctgcggt gcagccagcg ccgctgcaga actggtgagt aacagaaacg ccagaatact
    53221 ttttttcatc attttcgtaa tccatcaaaa aggttcgaga gtcgcaagca ggctgatgat
    53281 caccagcact accgcaccta tcgcccattc gagtttagtc atccacacaa accagggtgc
    53341 cgcacgatct tcatcctgcc tcatgcgcgg tacaagaaca taacgattag ccagcgcaat
    53401 gaccaccata atcatcacca ggatggcttt gagcaataag agttggcccc agtaagtcgt
    53461 aagcgtgggg ggaaatccgg taatcaacaa tgcattaagc acgccgcttg ccagtacgcc
    53521 gatcaccgca aaatgcccgc accaggaaaa acgcatcagc gcctgaatag cctggtgtcg
    53581 ccaacggcct ttgatgagtt gcatacacca gagcaccggc agtaatcccc caaaccacgc
    53641 cgccgcacaa atcaggtgaa tcgcgtgatt agtctgatgg attttcgccg ttaccccttc
    53701 attcagcgtc gcatgcccca ctcccgccag cagaataaat tgcgcggtag tgagcataaa
    53761 cagcaaacgt ggcatattcc gcggttgcat aagggcgacg atcaacgtta cgagggcgag
    53821 aacaatttgc cataaccaga ccccaccaaa ctgcgtttgc aaaacagcct gccaaatgtt
    53881 tggcgaaaat acatctgtcc atcccgttcc catcaacccg ccctgaacgg cgagcattgc
    53941 agtggcactg atcaaactcc agacggcagc gtgctgttgt aaacgcagaa aacgcttcgt
    54001 cagcaagcga cgaatcgtta agggggccag ccaggcaccg tacattgcga agccaaaaac
    54061 cagcatcagc gaggtgaaat ggataaatcg cagcgcgatc caggtaaacg ccagcatgat
    54121 ttatttcacg ctaaaggtgt agtgtccttt cgttttatgc ccgtccaccg aaacaacacg
    54181 ccagtcaacg gtgtaggttc cgggtttcag cgaatcggcc agtggaacaa tcagttgctt
    54241 ttggtcctgc tcatttcgct tcgccggtaa tgttttaata ttttcgtttt ttggccccgt
    54301 gatttttgca ccactgaatc ctgtttcaac accttccgag aagtttaaag tgattgcctg
    54361 cggggcagct gtcacttgcg cgtttgccgc aggatactga tgcgttaaat gcgcatgtgc
    54421 ccagacggaa ggtgtcacca acgaagtggt caggattgcc agcgcgtagc gaagggagcg
    54481 tgcagttgaa gccatattat ctattccttt ttgtaataac ttttttacag agcataacct
    54541 tgtctaatgt ctgagtcgag gatcatcaat tccggcttgc catccaggct cactcttagt
    54601 aacttttgcc cgcgaatgat gaggagatta agaatgctga agaatctggc taaactggat
    54661 caaacagaaa tggataaagt gaatgtcgat ttggcggcgg ccggggtggc atttaaagaa
    54721 cgctacaata tgccggtgat cgctgaagcg gttgaacgtg aacagcctga acatttgcgc
    54781 agctggtttc gcgagcggct tattgcccac cgtttggctt cggtcaatct gtcacgttta
    54841 ccttacgagc ccaaacttaa ataaaactta tacagagtta cactttctta cataacgcct
    54901 gctaaattat gagtattttc taaaccgcac ccataatttg cagtcatttt gaaaaggaag
    54961 tcattatgtc gttctggaaa gttgcagcag cgcaatatga gcctcgtaaa acctcactca
    55021 ccgaacaggt agctcatcat ctggagtttg tgagggccgc cgccaggcaa caatgtcagc
    55081 tgttagtttt tccatcgctt tctttactgg ggtgtgatta ttcgcgacgt gcccttcctg
    55141 ccccacccga tctctcactg ttagacccgc tttgttatgc cgcaacgacc tggcgaatga
    55201 ccatcattgc cggccttccc gttgaatata acgatcgctt tattcgtgga attgcggtgt
    55261 tcgcaccgtg gcgaaaaacg cctgggatct accatcagag tcatggtgca tgtctgggcc
    55321 gtcgttccag aaccatcacg gtggtcgatg aacaaccgca aggtatggat atggacccga
    55381 cctgttcact gtttacgaca gggcaatgtc tgggagaacc cgacctgctg gcgtcggccc
    55441 gtcgcttaca atttttttca catcagtact cgattgccgt gctgatggca aatgcccgtg
    55501 gtaacagtgc actatgggat gaatatggtc gtctcatcgt tcgcgccgat cgtggttcat
    55561 tattgttagt cggtcagcgt tcatcacagg gttggcaagg cgatatcatt ccattacgct
    55621 aggctttttc ggcctggagc atgccatgtt gcgcattatc gatacagaaa cctgcggttt
    55681 gcagggaggg atcgttgaga ttgcctctgt tgatgtcatt gacggaaaaa tcgtcaaccc
    55741 catgagccac ctggtgcgcc ccgatcgtcc tattagtcca caagcgatgg cgattcatcg
    55801 catcaccgaa gccatggtcg ccgataaacc gtggattgaa gatgtgatcc cacactatta
    55861 cggtagtgaa tggtatgtcg cgcataacgc cagctttgac cgccgcgtac tgcctgagat
    55921 gcccggtgag tggatttgca ctatgaaact ggcccgtcgt ttgtggccag ggatcaagta
    55981 cagcaatatg gcgttatata aaacacggaa gctcaatgta cagacgccgc cgggcctgca
    56041 tcatcaccgc gcgttgtatg actgttatat caccgccgcg ttgcttatcg atattatgaa
    56101 cacctccggc tggacggcag aacagatggc cgatatcacc ggacgtccgt cgttgatgac
    56161 gaccttcacc tttggcaaat accgtggcaa agcggtttcc gacgttgccg aacgcgatcc
    56221 gggctatctg cgctggttat ttaataacct ggacagcatg agcccggagc tgcgtttaac
    56281 actgaaacat tatctggaaa atacttaatc cgcaggcgta gcgggtaatg ttccctgcgc
    56341 cagcgcgacc agaaaagcat attccatcgc tacgccttcg tacgatttaa agcgaccaga
    56401 tttaccgcca tggcctgagt ccatgtcggt acagagcaat aaaagatggt catcggtttt
    56461 cagctcgcgc aatttagcga cccattttgc cggttcccaa tattgcacct gagaatcgtg
    56521 caaaccggtc gttaccagta aatgcggata agcctgtgcg gtgacgttgt catatgggct
    56581 gtagcttttc atgtactcgt aatattgcgg atcctgcgga ttcccccact cttcaaactc
    56641 accagtggta agaggaattg attcatcaag catcgttgtt acaacatcaa caaacggtac
    56701 ctgggcgata acgccgtgga ataattccgg gcgttgatta attgcaacgc ccatcaacat
    56761 gccccccgca ctcccgccca tcgcataaca aagcgaagga gagccatagc ccagttttaa
    56821 caatgcatcg caggcatcaa gataatcatt aaacgtattt ttcttcttca gaaatttacc
    56881 gtcttcgtac cattgttgcc ccagctcacc gccgccgcga acatggacaa tggcgtagac
    56941 aaaaccacga tctaacaaac tcaagcggct aaaactgaaa tcggcatcaa tacttgcgcc
    57001 gtaagaacca tagccataca ccagcaacgg gttgtgccct ttgcgaaaat gtttgcgatg
    57061 gtagaccaac gaaaccggaa cttcgacgcc atcacgggcg actatccaca ggtgttcact
    57121 gcggtaattc gccgcatcaa aaccaggaac ttccgtttgt tttaatacac gacgctcacc
    57181 ggtatccata tccagttcaa acaaagtgtc tggtgtggtc atggaagaat aaccataacg
    57241 caatcgcgcg gtttcaggtt ctggattgta ggcaatccag gtcacatagg ccggatcatc
    57301 aaaggcaata ccaatgactt cccgggtctt gcggttaatt tggcgcaaac tggttaaccc
    57361 gcgctgacgc tcttcaacca ccagccagtc ggtaaacagc gtaaaccctt ccagcatgat
    57421 gttttcgcgt ggcggaatta actcttccca ctgttgctca tcacgcatac gggtacggta
    57481 taagccaaag tttttgccgt tgcggttgga acgcagataa aaacgatgct ggtagtgatc
    57541 aaggctgtat tcgtgatctt tgcggcgcgg cagaaaaaca aacggctcgg catcggccat
    57601 ttccgcgtcc agcaggcgaa cttcactggt ggtggcgctg gccaaatgaa tgactacata
    57661 gtgcttcgag gtcgttttat gcaggctgac gtaataggta tcgtcttttt cttcgtaaat
    57721 cagtttatct tgcgatgctg gcgtaccgat ggcgtgacgc cagacctgat aaggcagcag
    57781 cgtcaccgga tgcttgcgaa catagtagaa agtccaggag tcatttgccc agacaaagct
    57841 gggttcaacg ttatccagca gttccgggta ccagttacct gtttccagat tacgaaaacg
    57901 aatgccgtac tggcgtcggg aaagaaaatc ttctgccagc gccataatgg tgttatcggg
    57961 cgtaatcgcc attccgccca tcgaataaaa ctcactatga gccgcgcgct tgttggcatc
    58021 gagcaatatt tcccactcgt cccactcttc actgaacgcc gattgacgct ggtagatagc
    58081 atattcacag cctggttcat aaatatgccg atagcggtag ccatttttga tgtagggcgc
    58141 agaaacttct ctttgcggaa tgcggtcgat gatttccttt aagatgcgat cctgcaaggc
    58201 ttgttgtgag gccatcaccc gatgaccgta actattttct tgttgcaggt agtccaggac
    58261 ttccggctga gaacgcgtat cgtcccgcag ccagtagtaa ttatcgatgc gcgtatcgcc
    58321 atgaagcgtc atggcgtggg gaatgcgggc ggcttttggt agcatgttat tgttctttct
    58381 ggttgaaaca tcttataagg gtggcaaaac tcaccgggga tgcaagcgaa acagggcagt
    58441 cattgcttag atgatgacag gtaatggcgc gaatatcgaa tgttatgcag acagagaaaa
    58501 tcagcctgtt caaatggctg tgcggttctg gatagcccga aatagtcaac ttcaggctat
    58561 ccagagagcg ggattattcc gccaaagtgc gtttttgctg ttcgagatcg cgttcaatgc
    58621 cttcacgaac atcctggggg attttcagcg cgtcacccag tgcattcagg taactgcgtt
    58681 ccataaaatg gtcaatatca atagccgcgc agctcaggaa atagatttcc agcgcctctt
    58741 cttcattgcg gaccccggta gccaggcgtt gtggatccag cggttgttcg attgcctgct
    58801 caatgagtac ccgcccctgc tcttccacgc cggcttcacg caattgctgg tcgattgccg
    58861 cacgttcttt ggcatcaata tgaccatcac ttttagcggc aaagaccagc gcaaggatca
    58921 aacgttccgt acgctcatcg agcggcgtac tttgcgcgcc aaactgcggt tcgtcctgat
    58981 gcgccgcgcg aattttatct ttgtatttat tccacagcac cgtacccgct accgctccgc
    59041 cgccaaccag taacgcgttg gtgccatatt tcgtaagaag tttacgtgct gatttatttg
    59101 cgaccagcag cccagccagt ccgcctaatg cgcctggcac taacagtttg cccaatccct
    59161 gatccgcaga cgaggaggta gaagaactgc tttgcccaag cagggattgc agttgattta
    59221 accagttagc catatttgct cctcaataac catttttagt gcctgtagca tagcggatga
    59281 ggatgtcaga aaatgtatat cgagacaaaa gatgcgcaaa taagcggggc ggttggccgt
    59341 tattcaaaga aattcgcgcc aggtaaggtg tttactatat atcaggtgat aagggtattt
    59401 taggtgaaag tagtttgacg ttttgagtgg cactgtatga tgctgattgc ccgatgctac
    59461 gctgttgcgt cttctcaggc ctacgattgt ggtgtgttgt gggcggacaa ggcactcgcg
    59521 ccgcatccgc caacggtcaa tcgaattatc agaagccatt aacgccgctg atattccgcc
    59581 attcccgctt tgcagtccac gctgacctgg taatgaatat cggcactttt accacgtacg
    59641 gttagcggta ctgaccattt atcatcttta ccctgaatgt cctgcaaact gacccatacc
    59701 acgggatcgg cctgaccgac aattttttga tcatctgccc agcgcgccac gcgattttgt
    59761 tgataatcac gttttacgct cgcggcaatt ccggcagcat ccagaccttc acactttggg
    59821 aaagtgaccg acttgctggt ttcattattg gcagcgaaaa ctgatgcgca gacagaaacc
    59881 aacaacagcc ctaaaaacgc ccctcttttt ttcatgtttt tctccatagc acaatgattc
    59941 aggagaaagc atggtacaaa ttgtcaggag cgcaagttgc ttcaggccgc gtgtgaggca
    60001 tcttcctctt cggattcagc accgacgacc tgtggggacg gtaatttgcc ggttttcaga
    60061 atggtgctca gcacatcttt ttgttctgcc agccatagcg aaagggcttc acgttgctcg
    60121 tcttccattt gcactggaga atttgtcagc caggtgtcaa gcagatccgc cgtatcaagc
    60181 attttgtcat aagcatcggc ttcctttttg ctggtaaacg acattttctc ttcgccctca
    60241 cgaatgacta cgtatttaac ttcaaccgcc atttgcagcc tctcataata actgtgattt
    60301 tatacagtat atttcttttc ggttgagaaa tcaacaccag caataaagac acacgcaaac
    60361 gttttcgttt atactgcgcg cggaattaat caggggatat tcgttatgac gttattaggc
    60421 actgcgctgc gtccagcagc aactcgcgtg atgttattag gctccggtga actgggtaaa
    60481 gaagtggcaa tcgagtgtca gcgtctcggc gtagaggtga ttgccgtcga tcgctatgcc
    60541 gacgcaccag ccatgcatgt cgcgcatcgc tcccatgtca ttaatatgct tgatggtgat
    60601 gcattacgcc gtgtggttga actggaaaaa ccacattata tcgtgccgga gatcgaagct
    60661 attgccaccg atatgctgat ccaacttgaa gaggaaggac tgaatgttgt cccctgcgct
    60721 cgcgcaacga aattaacgat gaatcgcgag ggtatccgtc gcctggcggc agaagagctg
    60781 cagctgccca cttccactta tcgttttgcc gatagcgaaa gccttttccg agaggcggtt
    60841 gccgccattg gctatccctg cattgtaaaa ccggtgatga gctcttccgg caaggggcag
    60901 acgtttattc gctctgcaga gcaacttgct caggcatggg aatacgctca gcaaggcggt
    60961 cgcgccggag cgggccgcgt aattgttgaa ggcgtcgtta agtttgactt cgaaattacc
    61021 ctgctaaccg tcagcgcggt ggatggcgtc catttctgtg caccagtagg tcatcgccag
    61081 gaagatggcg actaccgtga atcctggcaa ccacagcaaa tgagcccgct tgcccttgaa
    61141 cgtgcgcagg agattgcccg taaagtggtg ctggcactgg gcggttatgg tctgtttggt
    61201 gtcgagctgt ttgtctgtgg tgatgaggtg attttcagtg aggtctcccc tcgtccacat
    61261 gataccggga tggtgacgtt aatttctcaa gatctctcag agtttgccct gcatgtacgt
    61321 gccttcctcg gacttccggt tggcgggatc cgtcagtatg gtcctgcagc ttctgccgtt
    61381 attctgccac aactgaccag tcagaatgtc acgtttgata atgtgcagaa tgccgtaggc
    61441 gcagatttgc agattcgttt atttggtaag ccggaaattg atggcagccg tcgtctgggg
    61501 gtggcactgg ctactgcaga gagtgttgtt gacgccattg aacgcgcgaa gcacgccgcc
    61561 ggacaggtaa aagtacaggg ttaaacccgg caaaaaaacg ctacaaaaat gcccgatcct
    61621 cgatcgggca ttttgacttt tacagcttag cgccttctac agcttcacgc gccagcttag
    61681 taatgcggtc gtaatcgccc gcttccagcg catctgccgg aaccagccag gaaccaccga
    61741 tgcacagcac gcttttcagc gccaggtagt cacggtagtt agccggagaa ataccacccg
    61801 tcgggcagaa acggacctgg gagaacggac ccgcgatcgc ctgcagggct ttcacgccgc
    61861 cgttagcttc agccgggaaa aatttgaact ctttcaaacc gtagtccata cccagcatca
    61921 gttcggaaac agtgctgatc cccggaatca gaggaatagt cccttcggta gcagctttca
    61981 gcagcggctc ggtcagaccc gggctaattg cgaactgtgc acccgcttcg gtgacttctg
    62041 ccagctgctg tggattcagc accgtaccgg cacccacaat cgcttcaggc acttctttgg
    62101 cgatagcacg gatagcgtca actgcacact cggtacgcag agtcacttcc agaacgcgca
    62161 ccccaccagc aaccaacgct tttgccatcg gcaccgcgtg ttccagtttt tttaccacga
    62221 taaccggtac aaccgggccg gtggtcagga ttgattctgc acttgttttc cagtttttca
    62281 tcagagtttt ctctcgcctg attacaaatt tgtcgtctta aaaagtgata caggttgcgc
    62341 cctgttcggc accggacagt ttttcacgca aggcgctgaa taattcacgt cctgtcccca
    62401 cgcgtgacgc gctcaggtca ggaatgtgcg gttcgcgagc agccagttcc gcttcgtcta
    62461 ccagcagcgt cagttcgcct gtctgtccat tcacacgaat gatgtccccg tcgcgcactt
    62521 ttgccagcag cccgccatcg taggcttctg gtgttacgtg gatagctgac ggcactttac
    62581 ctgaagcgcc ggagagtcgt ccatcggtaa ctaacgcaat tttgaaacac cggtccaata
    62641 atacaccaag tggcggcatg agtttatgta attctggcat tccgttcgct tttggcccct
    62701 gatgacggac aacaacgaca caatcgcggt ccagcaaacc cgcttcaaag gccggcataa
    62761 cgtcatgctg gctttcaaaa acaaccgctg gcgcttcaat cacctggttc tcaaccggca
    62821 cggcagaggt tttcataacc gcacggccca ggttaccgct taacactttt gtcccaccat
    62881 gatgagagaa aggttgttcg aaggaagcga tcacattgct gtcgagtgat ttttccgccc
    62941 cttcccgcca gtccagttca ccattattca gccatggttc aagggtataa cgagacagac
    63001 caaagcctgc caccgtattg acatcttcat gcagcaggcc tgctttgagc agttcacgca
    63061 ccagaaccgg tacgccacct gccgcctgga agtggttaat atcggccgga ccgttcgggt
    63121 agagacgagc catcagcggt acaacatcag aaaggtcaga gaagtcatcc cagttaatct
    63181 gaataccggc cgcgcgcgcc atcgccacca ggtgcatggt gtggttagtg gaaccaccgg
    63241 tcgccagcag tgcaacgata ccgttcacca ccactttctc atcgatcatc ttaccgatcg
    63301 gcatccattc attaccatta ccggtcatgc gtgtaacctg acgcgcagcg gcagcggtca
    63361 aagcatcgcg cagcggagaa tccggatgaa caaaagaaga gcctggcaac tgcatcccca
    63421 taaactccac caccatctgg ttggtgttgg cagtaccgta gaaagtacat gttcccggcg
    63481 catggtaaga cgcggcttct gactccagta aggccatgcg gtccacttta ccttcggcat
    63541 aaagctggcg aatacgcact ttttctttat ttggcaaacc gcttgccatc ggtccagacg
    63601 gcacaaacac cgcaggcaaa tgaccaaacg acagggctgc catcgtcaga cccgggacaa
    63661 tcttgtcgca caccccgagg aacagagcac catcaaacat gttatgggac agcccgaccg
    63721 ccgcagacat cgctatcact tcgcggctta gcagcgacaa ctccattcca tcctgcccct
    63781 gggtgacacc atcacacatc gccggaacac cgcccgcaac ctgaccaacc gcattcgctt
    63841 catgcagggc tttacgaatg atttctggat agtgttcata aggctggtga gcggagagca
    63901 tgtcgttata ggaggtgatg atggcgatat tgttacgcaa catgcttttc aaagaggctt
    63961 tgtcttctgg ctggcaggca gcgaaaccgt gtgccaggtt accgcatgcc aactgcgaac
    64021 gatgaacggt cgacgttttc gcttgttcta tccgggcgag ataagcagag cgagtctcgc
    64081 gcgaacgttc aatgattcga tttgttacgc gtaacaattg tggattcata aaggctcctg
    64141 aaaatgagtt gtcagagcag gatgattcac aatgcgtttc attcagagga tttatgagtg
    64201 aaacgcctgt aaccggagct catagggcaa aaacgtttca gtcagtgtaa taaaaaaagc
    64261 ctcgtgggtg aatccgcacg aggcctgaaa gtgtaaaaat tgttctacaa tctgtgcaag
    64321 atcatgttac cggtaaaata accataaagg ataagcgcag atattactca aactcattcc
    64381 aggaacgacc atcacgggta atcatcgcca ccgaggcaac gggtccccag gttccggcct
    64441 gatacggttt cggcgcatca ttgtccatcg cccacgcctc agtaatggag tctacccatt
    64501 tccaggcttc ttccacttcg tcgcgacgta caaacagtgc ctgaatacca cgcatggttt
    64561 ccagcagcaa acgttcatag gcatccgcca gatgcgtctg attaaaggtt tctgaatagc
    64621 tcagatccag cttggtgatt tgcaggttat gtttgtggtc aaggccagga actttattca
    64681 gtacctggat atccacgcct tcatcaggtt gcagacggat agtcagttta ttctgcggca
    64741 gatcctgcca cgactcttta aacagattca gttcaggtgt tttgaaatag accacgactt
    64801 cagaacattt ggtcggcaga cgtttaccag tacgcaggta gaatggcaca ccggcccagc
    64861 gccagttatc aatgtcgacg cggatcgcca cgaaggtttc tgtattgctg ctcttgttcg
    64921 cgccctcttc ttccagatat cccggcactt ttttgccctg ggcgaagccc gcagtatatt
    64981 gcccgcgtac ggttttttcg cgtacgttgg agcggtcgat gcggcgcaga gacttcagta
    65041 ctttcacttt ttcatcgcgg atgctgtctg cgctcaggtc agacggcgga gacatcgcaa
    65101 tcatgcaaag aatttgcagc aggtggttct ggatcatgtc gcgcatctga ccggctttat
    65161 caaaatagcc ccagcgccct tcgatcccca cttcttctgc cacggtaatc tcaacatgat
    65221 caatggtgcg attgtcccag ttattcacaa acagggagtt agcaaaacgc agcgccaaca
    65281 ggttcagcac cgtttcttta ccaagatagt ggtcgatacg gtaaacctgg cactcctcga
    65341 agtattcgcc aacctgatca ttgatttcct gcgaggtcgc cagcgacgtc cccagcggtt
    65401 tctccatgac tacgcgtgcc ggtttagcat tcagttttgc ctcgccaagc cctttgcaaa
    65461 ttgcgccaaa agtgctgggc ggcatggcaa agtagttaat ggtgatacga tttttttgat
    65521 ccagcatcgc accgagacgg ttgaatgcag cagtgtcatt gacatcgaga ttacaaaaat
    65581 ccagacgtgc actcagggtg tcccataaac cttcatcaat ggtttctttc atgaaagttt
    65641 cgagcgcctc gcggacaact ttggtatatg ccgctttatc ccagtcagca cgccctacgc
    65701 cgataatccg ggtgtccggg ttgagctgac cggctttttc cagttgatac agggaaggca
    65761 gcaatttacg acgcgcaagg tcgcctttcg cgccgaaaat gaccaggtca caggcctggg
    65821 ctgtttgcgt taccgccatg tcattctcct taagttaact aacccggtac ttaagccagg
    65881 gtatacttgt aattttctta cggtgcactg tactgctttt acgagcttgc gaaaactgta
    65941 aacgcttatc cacccgtgcg attacggtaa aagcgcgcaa agtgcggcaa aactgataaa
    66001 aaaatcatcg ttttccattg ggtaaaaatc tgacactgat catgttatga aaaaaaataa
    66061 caactttttt atctgctttt gtcattaaca cagcacacag gcgtaatatc tgacaaaact
    66121 gcatttcgat ttctttcagt gcggaaatcg tcattacccg tgagtctctt tacatcatga
    66181 atatgctgga aaaaatccag tctcagctgg aacatttgag caaatcagag cgcaaagttg
    66241 ccgaggtcat tctggcttcg cccgataacg cgatccattc gagtattgct gctctggcac
    66301 tggaagccaa tgttagcgaa ccgacggtga atcgtttctg tcgcagcatg gacacgcgcg
    66361 gttttcctga ttttaaactt catctggcac aaagtctggc gaatggcact ccctatgtta
    66421 atcgcaacgt caatgaagat gacagcgttg aatcatacac ggggaaaata tttgagtccg
    66481 caatggcaac gctcgatcat gtccgtcatt cactggataa atctgccatc aaccgcgccg
    66541 tcgacttgct cactcaggca aaaaaaatcg cctttttcgg attaggctct tcagccgccg
    66601 ttgcccacga tgcgatgaat aagttctttc gttttaatgt tccggtggtg tactccgatg
    66661 atatcgtgct gcaacgcatg agttgtatga attgtagcga cggagacgtg gtggtgctga
    66721 tttctcacac tggaagaaca aaaaatctgg tcgagctggc gcagctggca cgcgaaaacg
    66781 acgctatggt gattgccctc acctctgcgg gtaccccgct cgcccgggaa gcaacgctgg
    66841 caattaccct cgacgtaccg gaagatactg acatttatat gcccatggtt tctcgacttg
    66901 cacagctgac cgtgatagat gtgctggcga caggatttac tttgcgacgc ggtgcaaaat
    66961 tcagagataa cttgaagcgg gtcaaggaag cgctgaagga atcgcgtttt gataagcagt
    67021 tacttaattt aagtgacgat cgctaaaagc gactgtcact gtcctaatct tatacgacat
    67081 ccgaatgaga ttaatttatc gccatcgcgg cgttatttca ttcggatttc atgttcaagc
    67141 aacacctggt tgtttcagtc aacggagtat tacatgtcca gaaggcttcg cagaacaaaa
    67201 atcgttacca cgttaggccc agcaacagat cgcgataata accttgaaaa agttatcgcg
    67261 gcgggtgcca acgttgtacg tatgaacttt tctcacggct cgcctgaaga tcacaaaatg
    67321 cgcgcggata aagttcgtga gattgccgca aaactggggc gtcatgtggc tattctgggt
    67381 gacctccagg ggcccaaaat ccgtgtatcc acctttaaag aaggcaaagt tttcctcaat
    67441 attggggata aattcctgct cgacgccaac ctgggtaaag gtgaaggcga caaagaaaaa
    67501 gtcggtatcg actacaaagg cctgcctgct gacgttgtgc ctggcgacat cctgctgctg
    67561 gacgatggtc gcgtccagtt aaaagtactg gaagttcagg gcatgaaagt gttcaccgaa
    67621 gtcaccgtcg gtggtcccct ctccaacaat aaaggtatca acaaacttgg cggcggtttg
    67681 tcggctgaag cgctgaccga aaaagacaaa gcagacatta agactgcggc gttgattggc
    67741 gtagattacc tggctgtctc cttcccacgc tgtggcgaag atctgaacta tgcccgtcgc
    67801 ctggcacgcg atgcaggatg tgatgcgaaa attgttgcca aggttgaacg tgcggaagcc
    67861 gtttgcagcc aggatgcaat ggatgacatc atcctcgcct ctgacgtggt aatggttgca
    67921 cgtggcgacc tcggtgtgga aattggcgac ccggaactgg tcggcattca gaaagcgttg
    67981 atccgtcgtg cacgtcagct taaccgcgcg gtaatcacgg cgacccagat gatggagtca
    68041 atgattacta acccgatgcc gacgcgtgca gaagtcatgg acgtagcaaa cgccgttctg
    68101 gatggtactg acgctgtgat gctgtctgca gaaactgccg ctgggcagta tccgtcagaa
    68161 actgttgcag ccatggcgcg cgtttgcctg ggtgcggaaa aaatcccgag catcaacgtt
    68221 tctaaacacc gtctggacgt tcagttcgac aatgtggaag aagctattgc catgtcagca
    68281 atgtacgcag ctaaccacct gaaaggcgtt acggcgatca ttaccatgac cgaatcgggt
    68341 cgtaccgcgc tgatgacctc ccgtatcagc tctggtctac caattttcgc catgtcgcgc
    68401 catgaacgta cgctgaacct gactgctctc tatcgtggcg ttacgccggt gcactttgat
    68461 agcgctaatg acggcgtagc agctgccagc gaagcggtta atctgctgcg cgataaaggt
    68521 tacttgatgt ctggtgacct ggtgattgtc acccagggcg acgtgatgag taccgtgggt
    68581 tctactaata ccacgcgtat tttaacggta gagtaagtac gttgccggat gcggcgaaaa
    68641 cgccacatcc ggcctacagt tcaatgatag ttcaacagat ttcgaatatt ctgaagcaaa
    68701 cttgaactta tcatcaggcg aaggcctctc ctcgcgagag gcttttttat ttgatgggat
    68761 aaagatcttt gcgcttatac ggctggattt cgcccggttt gcgagttttc agcaatttta
    68821 gtatccaggt gtattgttct ggtcgcggac caacaaaaat ctcgacttct tcattcatcc
    68881 gccgcgcaat cgtatgatca tccgcctcta acagatcatc catcggtggg cgcacctgaa
    68941 tcatcagacg atgcgtcttg ccatcataaa tcggaaacag cggtacaacg cgcgcacggc
    69001 acactttcat caaacgacca atcgcgggca acgtcgcttt ataggtggca aagaaatcca
    69061 caaattcgct gtgctctggg ccatgatcct gatcgggtaa ataatatccc cagtacccct
    69121 gacgtaccga ctggatgaat ggtttaatac cgtcatttct cgcatgcaga cgaccgccaa
    69181 agcgacgacg caccgtgttc cagacataat caaaaaccgg gttgccctga ttatggaaca
    69241 tcgctgccat tttctgccct tgcgaggcca tcagcatggc aggaatatcg acggcccaac
    69301 cgtgcggcac cagaaagata actttctcgt tattacgccg catctcttcg atgatctcca
    69361 gcccttgcca gtcaacgcgc ggctgaattt tctccggccc gcgtattgcc aactcagcca
    69421 tcattgccat cgcttgcggc gcggtggcaa acatctcatc aacaatcgct tcgcgttcag
    69481 cttcactacg ttctggaaag cagagcgaca gattgattaa cgcacgacgg cgtgagcttt
    69541 ttcccagtcg tccggcaaaa cgtcccagcc gtgccagaat gggatcacgg aactttggcg
    69601 gcgttaaagc gatacccgcc atcgctgcta cgcccagcca tgctccccag tagcgcgggt
    69661 ggcgaaagga tttatcaaac tcaggaatgt attcgctatt attttttttc gtttccatgc
    69721 ttttccagtt tcggataagg caaaaatcaa tctggtgata gtgtagcggc gcaacttgcc
    69781 ccgcaccaaa taaaaaaagc cggtactgac tacgtaccgg ctgcgaatgg atgttaatta
    69841 atcaaaccgt agctgcggca caatctcttt ggcctgcgcc aggaattcgc gacgatcgga
    69901 gccggtcagc ccttcggtac gcggcagttt tgccgtcagc gggtttacgg cctgctggtt
    69961 tatccatact tcatagtgca gatgcggccc ggttgaacgt ccggtattac cggaaagcgc
    70021 gatacggtcg ccacgtttca ccttctgtcc cggtttcacc agaatcttgc gcaagtgcat
    70081 ataacgcgtg gtgtagctgc gaccatgacg aatagccaca taataacctg ctgcgccact
    70141 gcgtttggca accaccactt caccgtcacc cactgaaagc actggcgtac cctgcggcat
    70201 ggcgaaatca acacctctgt gtggtgcaac gcgaccggtc accggattag tacgacgcgg
    70261 gttaaagtta gacgagatac ggaactgttt cgccgtcggg aatcgcaaga atcctttcgc
    70321 cagaccagta ccgttacgat cgtagaattt gccatcttca gcgcggatag cgtaataatc
    70381 tttaccttct gaacgcaaac gtacgcccag cagctggctt tgctcacgtt taccatcaag
    70441 catttctcga gacattaata ccgcaaattc atcgcctttt ttcagtttgc ggaaatccat
    70501 ttgccactgc atggctttaa tcactgcgct cacttcggcg ctggttaaac cggcgtttct
    70561 ggcgctggca acaaagctcc ccccgacggt acctttcagc agattgttaa cccactctcc
    70621 ttgctgcatt tcgctggtca ttttaaaacc gttagcggca gtacggtcat aggttcgggt
    70681 ttcacgacga gacacttccc aggtgaggcg ctgcagttcg ccgtccgcgg ttaatgtcca
    70741 ggagagttgt tgaccgattt tcaggttacg caattctttg tcggccgcag ccagttgggt
    70801 gatatcaccc atatcaatac catactgatt gagaatgctg cttagcgtat cgccagtgga
    70861 aacaacatat tcatgcacgc ccgcttcacc ggcgattttg tcatccagtt cgtcctgggg
    70921 aatggcttca tcttcttgtg cagcttgatc aatcggctca ctggcttcag gtaagagcga
    70981 acgaatttcg ttctgttcca gctcaatggt tttgacaatt ggcgtggcat cgcggtgata
    71041 aacatagggc cgccagacag cgacggccag agtaagaacg gtgagcgacc ccaacataac
    71101 gcggtgtggt cgcggtaaat tattaaacgc cagggcgaca gagcgggcta tctgttgcac
    71161 gtaatcactt cctcattaat ctcctttcag gcagctcgca tactggttgg ctaattgact
    71221 caggaattct gaatagcttg ttttacccag tttgatattc gtccccaggg gatccaacgt
    71281 tcccatacga acggatgtcc ctcgtgcgac gctctcaacg accgctggcc tgaactgtgg
    71341 ctcagcaaaa acgcaggttg ctttttgctc aaccaactgt gttcttattt catgtaaacg
    71401 ctgcgcgcca ggttgaatct cagggttaac ggtaaaatga ccaagcggtg tcagtccgaa
    71461 ctgtttttcg aaatagccgt aagcatcgtg aaaaacgaaa taacctttcc ccttgagcgg
    71521 cgcgagctcg ttaccaacct gcgtttcggt tgaggctaat tgtgcctcaa aatccttcag
    71581 gttggcgtca agtttggctc gactttgcgg cataagttcc actaattttc catggattgc
    71641 aaccgctgta gcccgcgcta tctctgggga aagccaaaga tgcatgttga aatcgccgtg
    71701 atggtgatct tcgtcacttt tttccgcgtg gtcgtgatca tcatcatcgc cgtgaatact
    71761 tttcatcagt agcggtttca catcttctag ctgcgcaatc gttacctgct tcgcttctgg
    71821 taatttgctt accggttttt gcataaacgc ttccatctcc gggccaaccc aaacgactaa
    71881 gtccgcgttc tgtaagcgtt ttacatccga tggacgcagt gaataatcat gttctgaagc
    71941 accgtcaggc agtaaaacct ccgtttctgt taccccatca gcaatggcag aagcgatgaa
    72001 cccaacgggt ttaagcgaag cgacaacggc ggcatctgcg gcctgtgttg cacctcccca
    72061 gagagcggcg gataatgctg cgaaaagaag cgttttttta tgtaacataa tgcgaccaat
    72121 catcgtaatg aatatgagaa gtgtgatatt ataacatttc atgactactg caagactaaa
    72181 attaacatga caagtctggt ttccctggaa aatgtctcgg tttcttttgg ccaacgccgc
    72241 gtcctctctg atgtgtcgct ggaacttaaa cctggaaaaa ttttgacttt acttgggcca
    72301 aatggcgcag gtaagtcgac actggtacgg gtagtgctcg ggctggtaac acccgatgaa
    72361 ggggttatca agcgcaacgg aaaactgcgc atcggctatg taccgcagaa gctgtatctc
    72421 gacaccacgt tgccactgac cgtaaaccgt tttttacgct tacgccctgg tacacataaa
    72481 gaagatattt tgcctgcact gaaacgtgtc caggccgggc acctgattaa cgcaccgatg
    72541 caaaagctct ctggtggcga aacgcagcgt gtactgttag cgcgagcatt gttaaatcga
    72601 ccgcaattat tagtgctgga tgaacccact caaggcgtgg atgtaaatgg ccaggtggcg
    72661 ttatatgacc ttattgacca actgcgtcgt gaactggatt gtggcgtttt aatggtttct
    72721 cacgatctgc atctggtgat ggcaaaaacc gatgaagtgc tgtgcctgaa tcaccacatt
    72781 tgttgttccg gcacaccgga agttgtttcc ctgcatccgg agtttatttc aatgtttggt
    72841 cctcgtggtg ctgaacaact gggtatctat cgccatcatc ataatcatcg tcacgattta
    72901 cagggacgaa ttgttttgcg tcggggaaat gatcgctcat gattgaatta ttatttcccg
    72961 gttggttagc cgggatcatg ctcgcctgtg ccgcgggtcc gctgggttcg tttgtagtct
    73021 ggcgtcgtat gtcttatttc ggtgatacgc tggctcatgc ctcattactt ggtgtcgcgt
    73081 ttggtttgtt gctggacgtg aatccattct atgcggtgat tgccgttacg ctgctgctgg
    73141 cgggcggtct ggtatggctg gagaagcgtc cacagctggc gatcgacacg ttattaggga
    73201 ttatggcgca cagtgccctg tcgctgggcc tggtggtcgt tagtctgatg tctaacattc
    73261 gtgttgattt gatggcttac ctgttcggtg atttactggc agtgacgcca gaagatctca
    73321 tctctattgc aattggcgtg gtcatcgtgg tggctatttt gttctggcaa tggcgcaatt
    73381 tgctgtcgat gacgattagc ccggatctgg cgtttgttga tggtgtgaaa ttacagcgag
    73441 tgaaattgtt gttgatgctg gtgacggcat tgacgattgg tgtagcgatg aaatttgtcg
    73501 gtgcgttgat tattacttcg ttgctgatta ttcctgcagc tactgcgcgt cgctttgccc
    73561 gtacgccgga acagatggct ggtgtcgctg ttttggtggg gatggtggca gtaactggcg
    73621 gtttaacctt ttccgcgttt tacgatacgc cggcgggtcc gtcggtggtg ctatgtgcgg
    73681 cactgttatt tattatcagt atgatgaaaa agcaggccag ctaatctatc gctgaacaca
    73741 tttgtcggat gcggcgcgag cgccttatcc gacctacggt tcgctatctc tggtaggcct
    73801 ggtaagacgc gaacagcggc gcgagcgcct tttccgccct acggttcgct atctctggta
    73861 tatgcctgat aagacgcgaa tagcgtcgca tcaggcacac tgccaatgtc ggatgcggtg
    73921 cgagcgacca atccgattta cggcatttct ggcggcgtta tgccaaagtg attccacgcc
    73981 cgcgtcgtcg ccatacgccc gcgcggtgta cgctgcaaaa agccttgctg aatcaaataa
    74041 ggttccagca catcctcaat ggtttcacgt tcttcgccaa tagccgccgc caggttatcc
    74101 agacctaccg gcccaccaaa gaacttatcg attaccgcca gcaacaattt gcggtccata
    74161 taatcgaaac cttcagcatc gacattcaac atatccagcg cctgagcagc gatatctgcc
    74221 gagatggtgc catcgtgctt cacttcggcg aaatcacgca ctcgacgcag cagacggttg
    74281 gcaatgcgcg gcgtaccgcg agcgcgacga gcaacttcca gcgcgccgtc atcactcatc
    74341 tcaagcccca taaagcgtgc gctgcgactg acgatatatt gcagatccgg cacctgataa
    74401 aactccagac gttgcacaat accaaaacgg tcgcgcaacg gtgatgtcag cgaacctgcg
    74461 cgcgtggttg caccaatcag ggtaaacggc ggcaaatcaa ttttaatgga gcgtgccgcc
    74521 ggaccttcac caatcatgat atccagttgg tagtcttcca ttgccgggta cagcacttct
    74581 tcaacaactg gcgataaacg gtggatctca tcaataaaca gcacgtcatg cggttcaagg
    74641 ttagtgagca tcgcagccaa atcgcccgcc ttttccagca ccggaccaga agtcgtgcgt
    74701 aaattaacgc ccatttcatt ggcgacaatg ttggcaagcg tagttttacc caaccccgga
    74761 ggaccaaaaa tcaacaaatg atcgagggca tcgccgcgca gtttcgctgc tttgatgaaa
    74821 atctccatct gtgaacgaac ctgcggctga ccaacatact cttccagtaa tttagggcga
    74881 atggcgcgat ctgctacatc ttccggcaaa gtggtaccgg cagaaatcag acggtctgct
    74941 tcaatcatcc tttacctcat aacgcggcgc gtagggcttc gcgaattaat gtttcactgc
    75001 tggcgtcagg gcgagcgatt ttgctcacca tgcggcttgc ttcttgtggt ttatagccca
    75061 gcgccaccag cgcggcaacc gcttcttgtt cagcatcgtc ggtcgccggg ctggcaggag
    75121 acgtgagtac caggtcggcg gctggcgtaa agagatcgcc atgcaaacct ttaaatcggt
    75181 ctttcatttc aacaatcaag cgttcggcgg tttttttgcc aatacccggc agtttcacca
    75241 gtgcccccac ttcttcacgc tcaacggcat taacgaactg ctgcgctgac attccggaga
    75301 ggatcgccag cgccaacttc gggccgacgc cgttggtttt gatcaactct ttaaacaatg
    75361 tgcgctcttg tttattgtta aaaccgtaca gcagttgcgc gtcttcacgc accacaaagt
    75421 gggtgaagac gatcgcttcc tgacccgctt cagggagttc ataaaaacag gtcatcggca
    75481 tatgcacttc atagcctacg ccgcccactt caattaacac cagcgggggt tgtttttcaa
    75541 tgatgatgcc tctgagtctg cctatcacat gacgctcctg cgtaatgaat caaagataat
    75601 gctgtatgat aaaaaaatgc tggatagata tccagcgaag gatgaagaaa acttgcgagg
    75661 tgtctcgatg aactgaaaaa tggcacagta taatttattc tacaggttat attggaagca
    75721 aatattttaa tattacatat tcagtgaata aatgcgtaat aaaaatatac attgcgcctc
    75781 ctgaaaaaat aaatttttta tgctattacg tatattcgta tctatttcaa tggaatgaca
    75841 acgtgaatat taattaccct gctgaatatg aaattggaga tatcgtcttt acatgtatag
    75901 gtgctgcctt atttggtcaa atatcagctg catcaaattg ctggagtaat cacgtcggga
    75961 tcattatcgg tcataacggt gaagacttcc tggttgcaga aagccgggtt cccctttcaa
    76021 ccatcaccac gctatctcgt tttattaaac gttcgtctaa tcaacgctat gctataaagc
    76081 gattagacgc tgggctgaca gaacaacaaa aacaacgaat tgttgaacag gttccctccc
    76141 ggctacgcaa actttaccac accggtttta aatacgaatc ttcgcgccag ttctgttcaa
    76201 aatttgtttt tgatatttat aaagaagcgc tatgtattcc ggtgggtgaa atagagacgt
    76261 ttggagaatt gttaaatagc aatccgaatg caaaactcac tttctggaaa ttctggttcc
    76321 tgggttctat tccgtgggag cgtaaaaccg tcacgccagc cagtttgtgg catcatccgg
    76381 gtttggtgtt gattcatgcg gagggagtgg aaacgcctca gccggaactg accgaggcgg
    76441 tataacttaa cgcagtcgcc ctctcgccag gttcagccgc gattcgctca tctgcatcgc
    76501 attctgacta acgtggcagt gggtgatggc aatcgccagc gcatcggcgg catccgcctg
    76561 tggattagcg ggcagtttca gcaaggtgcg gaccatatgc tgcacctggc ttttttcggc
    76621 actaccaata cctaccaccg tttgctttac ctgacgtgcc gcgtattcaa atactggcaa
    76681 ctcctgattc accgccgcca caatcgccac gccgcgcgcc tggcccagtt tcagggctga
    76741 gtcagcgttc tttgccataa agacttgttc aatggcgaaa taatcaggct ggaactgggt
    76801 gatgatttcc gtcacgcccg catagatgag tttcagacga gacggtaaat catccacttt
    76861 ggtgcggatg cacccgctac ccaggtagga cagttgccta cctacctggc ggatgacgcc
    76921 gtagccggtc acgcgcgaac ccggatcaat gccgagaata atagccatca cgcgtctccg
    76981 ttttgctgtt tagcaggcct catcagagag tcgctgcgac ctcatcagag atttcaccgt
    77041 tatggtaaac ttcctgcacg tcgtcgcaat cttccagcat atcgatcaga cgcatcagtt
    77101 tcggtgcggt ttctgcatcc atatcagctt tggtagacgg gatcatggaa acttccgcgc
    77161 tgtctgcttt cagacctgcc gcttccagag cgtcgcgcac tttacccatt tcttcccatg
    77221 cagtgtagac atcaatcgcg ccgtcatcat aggtcacaac gtcttcagca cctgcttcca
    77281 gtgctgcttc catgatggtg tcttcatcgc ctttctcgaa ggagatgacg ccttttttgc
    77341 tgaacagata ggctacggaa ccatcagtac cgaggttacc gccacatttg ctgaatgcat
    77401 gacgcacttc agcaacggta cggttgcggt tgtcagacag acattcaatc atgattgccg
    77461 tgccgccagg accgtaacct tcgtagatga tggtttccat gttcgcatca tcatcaccgc
    77521 ccacaccacg tgcaattgcg cggttcagag tgtcacgggt catgttgtta gacagtgctt
    77581 tatccaccgc tgcacgcaga cgcgggttag cgtccggatc gccaccgccc agcttagccg
    77641 cggttaccag ctcacgaatg attttagtga agattttacc gcgcttagca tcctgcgcag
    77701 ctttacgatg tctggtgttg gcccatttac tatgacctgc cataaaaata tctccagata
    77761 gccctgcctg ttcaggcagc gttaattaca aactgttcaa tcgcctgccg gttgctccag
    77821 gacttagtga gcgccgccgc agcaggcgca tcaagccact tgtaagccag atgttcagtg
    77881 aaaacgatct gccgctcgtg cggaagcgca agacagaacc atgattccgt attacgcgtc
    77941 acgcccggcg catagcgatg acgtaaatgt gaaaaaattt caaactcaac cgtgcgctga
    78001 cagtcaatta aggtcagttg ttcagcgaca acatcaatgg tgacctcttc ctttacttcg
    78061 cgcatggcag cttgcggcgc ggtttcaccc tcttccacgc tgccggttac cgactgccag
    78121 aaatcggggt catcacgccg ctgcaacatc agcacccgtt tcgtatcttg tgcgtagatg
    78181 accactaaga tcgaaacggg acgcttatac actttatcct tcacgctgcc tcttcgttga
    78241 ctgccttcgc tcatcctatt cacatagtta tctatgctca tgggagttca ctcagttgcc
    78301 gcctcgatgc aacgcgaatg atttcgtgta tttgactcat atcagttatt ctcagccttc
    78361 ttcacaacct gaatgctcag ctcagccagt gcagtcgggt tagcaaagct cggtgcttca
    78421 gtcatcagac acgctgccgc cgtggttttc gggaaggcga taacgtcacg gatattgtcg
    78481 gtgccggtca gcagcatggt cagacggtca agaccgaatg ccagacctgc gtgcggcgga
    78541 gtaccgtatt tcagagcgtc gagcaggaag ccgaatttct cgcgctgttc ctcttcgttg
    78601 atacccagaa taccaaacac cgtctgctgc atatcaccat tatggatacg tactgaacca
    78661 ccgcccactt cgtaaccatt gatgaccata tcgtaagcgt tcgccaccgc attttccggt
    78721 gcagctttca gctctgcagc cgtcatgtct ttcggtgagg tgaacggatg gtgcattgcc
    78781 gtcaggccgc cttcaccgtc gtcttcaaac atcgggaagt cgataaccca cagcggtgcc
    78841 catttgcttt cgtcggtcag accaaggtct ttacccactt tcaggcgcag tgcgcccatc
    78901 gcgtcggcaa caattttctt gttgtcggca ccgaagaaaa tcatatcgcc atcttgcgcg
    78961 gcagtacgat ccaggatggc ttcgatgatt tctgcattaa ggaacttcgc taccgggctg
    79021 ttgatacctt ccagaccttt cgcgcgttcg ttaactttga tgtaagccag acctttcgcg
    79081 ccgtagattt taacgaagtt accgtattcg tcgatctgct tacgggtcag cgatgcgcca
    79141 cccggaacac gcagagcggc aacgcgacct ttcggatcgt tcgccggacc tgcaaatact
    79201 gcaaactcaa cagatttcag cagatcggca acgtcggtca gttccatcgg gttacgcaaa
    79261 tctggtttat cggaaccgta acggcgttca gcttccgcaa aggtcattac cgggaaatcg
    79321 cccagatcca cgcccttcac ttccagccac agatggcgca ccagcgcttc catcacttca
    79381 cgcacttgcg gcgcggtcat gaaagaagtt tccacatcga tctgagtaaa ttcaggctga
    79441 cgatcagcgc gcaggtcttc gtcacggaag catttaacga tctgatagta gcggtcaaag
    79501 ccggacatca tcagcagctg tttgaacaac tgtggggatt gcggcagtgc gtagaattta
    79561 cctttgtgca cacgagaagg caccaggtag tcacgcgcgc cttccggcgt ggctttggtc
    79621 agcatcggag tttcgatatc gaggaagccg tggtcatcca taaaacggcg caccaggctg
    79681 gtgattttag cgcgggtttt caggcgctga gccatttccg ggcgacgcag gtcgaggtag
    79741 cggtatttca gacgcgcttc ttcggtgttg acgtggttag agtcaagcgg cagaacatct
    79801 gcgcgattga tgatagtcag cgaggacgcc agcacttcga tttcgccggt cgccatatcg
    79861 cggttaatat ttttttcgtc acgcgcacgt acggtgcccg tgacctgaat gcagaactca
    79921 ttacgcagtt cagaggccag ctttaacgcg tccgcacgat ccggatcgaa aaatacctgc
    79981 acgatacctt cgcggtcgcg catatcgatg aagatcaggc taccaagatc acgacgacgg
    80041 ttgacccaac cacacagagt cacctgctgc cccacgtggg acaaacggag ctgtccacaa
    80101 tattctgtac gcatgagata tcccttaact tagctgccgg cggatgcccc ctgctgcgca
    80161 ggtgaccaag tcgcagcgtt agctgtatgt cacaactgaa tgaaaaaagg cggctattat
    80221 actggaaatt ctgccgcacc gtaagagcct ggcccgcgct ggaacgcctc gttaccactt
    80281 tatatcgggc ctgaaatcag actctacgcc agtttgctat aaaggtgttg cccgaactca
    80341 taaaaattaa caaaatttgt cgttccgcca tcggctaatc gcattaaggt gtgaggcacg
    80401 attttgtttt ttcaggagtc atcatgcttg aacttaatgc taaaaccact gcgctggtgg
    80461 tgatcgattt acaagaaggc atcttacctt ttgccggtgg tccacatact gccgatgagg
    80521 tagttaatcg cgccgggaag ctggcggcga aatttcgcgc cagcggtcag cccgtgtttc
    80581 tggtgcgcgt tggctggtct gccgattacg ccgaagcatt aaaacagccg gttgatgccc
    80641 cttcccccgc taaagtgttg cccgaaaact ggtggcaaca tccagctgca ttaggtgcaa
    80701 ctgacagtga tatcgaaata atcaaacgtc agtggggtgc gttttacggt acggatctgg
    80761 agttgcaatt acaccgccga ggtatcgata caatagtgtt atgtgggatc tcgaccaata
    80821 tcggtgttga atccaccgcc cgcaatgcct gggaacttgg ctttaatctg gtgattgccg
    80881 aagacgcctg tagtgccgcc agcgccgagc aacacaataa cagcattaat catatctacc
    80941 cgcgcatcgc ccgtgtgcgt agcgtggaag agatccttca cgcgttatga tttacatcgg
    81001 tctaccgcaa tggtcgcatc ctaaatgggt gcggttgggg atcacccgcc ttgaagagta
    81061 tgcccgccac tttaactgcg tgacgcaaca tttttaaaaa tcgacgaaga tcgtccagga
    81121 gcgccgtttt tcaagggttg gatagatgat tcgtttacca tgtcgacgaa cttcgacgaa
    81181 tttcgccgat aaaaagtgtc ccatacatgc cccattgatg ccccataaac aacatcagga
    81241 attgacactg gttatccata cagataaaaa taacacctgt acataaccac agttacagga
    81301 gggtgttatg cgtgttgaga tctgtatcgc taaagaaaaa atcagcaaaa tgccaaaagg
    81361 ttctgttcct gccctgcaac aggaaatgct acgacgtgtc ggtaagcgtt atgacgatgt
    81421 agatgttatc gttaaatctg ccagcaatga tggactcact attttaagag ccgtggataa
    81481 agaatccgcg caagagtttg tacaagaaac gcttcaggaa gtctgggaaa cagcggatga
    81541 ctggtttatt cactaattaa tagatttgca ggtgatttcc gttacaggct tatcacctgt
    81601 aaaaccttac tagccgctta tctgtaacta tggaaaaacc gtaagtctgg ctttaaggtt
    81661 aacaaaagga tgtttatgcc gatttttcat aactgactgt tttatatcac aagtggagat
    81721 gactttatct acatcaacct cctttatgca aaggatacag gcgcaaaagg taatcatcag
    81781 gtcagacctt atattccctc aaaatctttg aaacgctgat tccatctatt aatcatactc
    81841 gcactctgaa cccttcagtc ttccttactg aacatgtatc gtcacatggt tgacccgata
    81901 cagaatcaca cgcaatttat tacaacaacc attatgttgg taagacctga aacaaaaaag
    81961 aatcaggagc aggggaaaag cagcccattg caggatactg aggtgcgact cattatccag
    82021 ttcaccaggc tctaagtgag cactacacac tacagaactg acaggagcgt ttctggcaat
    82081 cctagttcac caactggtga tttatgttga tttctcgtca gaaacgcttc atggacacat
    82141 caaactccct aaaaccagtg aacaagcaaa taaattttca taattgctca caacctttct
    82201 acaaagacaa catcaatatt tttatagt
//
LOCUS       DAZFKF010000009        78455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFKF010000009 DAZFKF010000000
VERSION     DAZFKF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 78455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.009"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            38..4600
                     /gene="sslE"
                     /locus_tag="ACHKA1_000756"
     CDS             38..4600
                     /gene="sslE"
                     /locus_tag="ACHKA1_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFO5678456.1"
                     /translation="MNKKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGS
                     LPEVKPDPTPNPEPTPEPTPDPEPTPEPIPDPEPTPEPEPEPVPTKTGYLTLGGSQRV
                     TGATCNGESSDGFTFKPGEDVTCVAGNTTIATFNTQSEAARSLRAVEKVSFSLEDAQE
                     LAGSDDKKSNAVSLVTSSNSCPANTEQVCLTFSSVIESKRFDSLYKQIDLAPEEFKKL
                     VNEEVENNAATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPSEIILSE
                     GRLVDSQGYGVAGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTI
                     ALTELGDEVRGANIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNG
                     ATLGEGEQVVNLPNEFIEQFNTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQ
                     GVINKLWGVDEDYKSVTKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKN
                     YWLAFGEKRAWDKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIGN
                     PHYNSILRCPNGYSWNGGVNKDGQCTLSGDSDDMKHFMQNVLRYLSDDKWTPDAKASM
                     TVGTNLDTVYFKRHGQVTGNSAEFGFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFE
                     YVTQVGNDPYAIPLRADTSKPKLTQQDVTDLIAYMNKGGSVLIMENVMSNLKEESASG
                     FVRLLDAAGLSMALNKSVVNNDPQGYPDRVRQRRSTPIWVYERYPAVDGKPPYTIDDT
                     TKEVIWKYQQENKPDDKPKLEVASWQEEVEGKQVTQFAFIDEADHKTPESLAAAKKRI
                     LDAFPGLEECKDSDYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAA
                     DLGTNIQRLYQHELYFRTNGRKGERLSSVDLERLYQNMSVWLWNDTSYRYEEGKNDEL
                     GFKTFTEFLNCYTNDAYTDGTQCSADLKKSLVDNNMIYGESSGKAGMMNPSYPLNYME
                     KPLTRLMLGRSWWDLNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTGL
                     WAPAQKEVTIKSNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVKF
                     KVPYGGLIYIKGNSSTNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESASFVY
                     TTPKKNLNASNYTGGLEQFANDLDTFASSMNDFYGRDETSGKHRMFTYKALTGHKHRF
                     TNDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGAT
                     EVANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNNQAWARGGAGDRLLMYAQLKE
                     WAEKNFDIKKWYPDGTPLPEFYSEREGMKGWNLFQLMHRKARGDDVGNDKFGNRNYCA
                     ESNGNAADKLMLCASWVAQTDLSEFFKKWNPGANAYQLPGVSEMNFEGGVSQSAYETL
                     AALGLPKPQQGPETINQVTEHKMSAE"
     gene            4927..5736
                     /gene="pppA"
                     /locus_tag="ACHKA1_000757"
     CDS             4927..5736
                     /gene="pppA"
                     /locus_tag="ACHKA1_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO5678457.1"
                     /translation="MLFDVFQQYPVAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            5802..6212
                     /gene="gspS2"
                     /locus_tag="ACHKA1_000758"
     CDS             5802..6212
                     /gene="gspS2"
                     /locus_tag="ACHKA1_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO5678458.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCAGHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQITQTPDAFLTSYQRQMCADPTVKLM
                     LTEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            6230..7189
                     /gene="gspC"
                     /locus_tag="ACHKA1_000759"
     CDS             6230..7189
                     /gene="gspC"
                     /locus_tag="ACHKA1_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO5678459.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSLNTDKEHLRKIARGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTVVSPSVNKPPRTDAKTFDKNDVQLISQQNWF
                     GKYQPVAAPVKQPEPAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLVEEERSTVAVTNKKAISDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            7219..9279
                     /gene="gspD"
                     /locus_tag="ACHKA1_000760"
     CDS             7219..9279
                     /gene="gspD"
                     /locus_tag="ACHKA1_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO5678460.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            9279..10772
                     /gene="gspE"
                     /locus_tag="ACHKA1_000761"
     CDS             9279..10772
                     /gene="gspE"
                     /locus_tag="ACHKA1_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO5678461.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDAHTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            10772..11995
                     /gene="gspF"
                     /locus_tag="ACHKA1_000762"
     CDS             10772..11995
                     /gene="gspF"
                     /locus_tag="ACHKA1_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO5678462.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPMHIEA
                     RMNASAGGMLQRRRHAHRRVASADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPATTRALIAM
                     SDALQASGVYWLAGLLALLVLGQRLLKNPVMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            12012..12467
                     /gene="gspG"
                     /locus_tag="ACHKA1_000763"
     CDS             12012..12467
                     /gene="gspG"
                     /locus_tag="ACHKA1_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO5678463.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADARNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            12471..13034
                     /gene="gspH"
                     /locus_tag="ACHKA1_000764"
     CDS             12471..13034
                     /gene="gspH"
                     /locus_tag="ACHKA1_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO5678464.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLSQCDERMP"
     gene            13031..13402
                     /gene="gspI"
                     /locus_tag="ACHKA1_000765"
     CDS             13031..13402
                     /gene="gspI"
                     /locus_tag="ACHKA1_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO5678465.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPQNTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            13399..14004
                     /gene="gspJ"
                     /locus_tag="ACHKA1_000766"
     CDS             13399..14004
                     /gene="gspJ"
                     /locus_tag="ACHKA1_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO5678466.1"
                     /translation="MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVTGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQENWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            14001..14978
                     /gene="gspK"
                     /locus_tag="ACHKA1_000767"
     CDS             14001..14978
                     /gene="gspK"
                     /locus_tag="ACHKA1_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO5678467.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLALQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            14975..16153
                     /gene="gspL"
                     /locus_tag="ACHKA1_000768"
     CDS             14975..16153
                     /gene="gspL"
                     /locus_tag="ACHKA1_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO5678468.1"
                     /translation="MSSILEIFFPLCASDTIRWQRRTPDVEHGIWPDVADERLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVPRKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGYMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPLSLTVVGIAPEELSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGSWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRDQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            16155..16691
                     /gene="yghD"
                     /locus_tag="ACHKA1_000769"
     CDS             16155..16691
                     /gene="yghD"
                     /locus_tag="ACHKA1_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO5678469.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLRSAAPDIIAARKSATTAPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(16840..16915)
                     /locus_tag="ACHKA1_000770"
     tRNA            complement(16840..16915)
                     /locus_tag="ACHKA1_000770"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16880..16882),aa:Phe,seq:gaa)
     gene            complement(17021..17728)
                     /gene="yqgA"
                     /locus_tag="ACHKA1_000771"
     CDS             complement(17021..17728)
                     /gene="yqgA"
                     /locus_tag="ACHKA1_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO5678470.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHEPF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            17881..17964
                     /locus_tag="ACHKA1_000772"
     CDS             17881..17964
                     /locus_tag="ACHKA1_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678471.1"
                     /translation="MHAISLHSLAYRRFARNPSLFMLLLMR"
     gene            18127..20262
                     /gene="speC"
                     /locus_tag="ACHKA1_000773"
     CDS             18127..20262
                     /gene="speC"
                     /locus_tag="ACHKA1_000773"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO5678472.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQHWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESQEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMTPQDAH
                     SAYIRGDVELVRIRDAEGRIAVEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(20312..21568)
                     /gene="nupG"
                     /locus_tag="ACHKA1_000774"
     CDS             complement(20312..21568)
                     /gene="nupG"
                     /locus_tag="ACHKA1_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO5678473.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(21770..22849)
                     /gene="mltC"
                     /locus_tag="ACHKA1_000775"
     CDS             complement(21770..22849)
                     /gene="mltC"
                     /locus_tag="ACHKA1_000775"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO5678474.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(22914..23189)
                     /gene="yggX"
                     /locus_tag="ACHKA1_000776"
     CDS             complement(22914..23189)
                     /gene="yggX"
                     /locus_tag="ACHKA1_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO5678475.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(23217..24269)
                     /gene="mutY"
                     /locus_tag="ACHKA1_000777"
     CDS             complement(23217..24269)
                     /gene="mutY"
                     /locus_tag="ACHKA1_000777"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO5678476.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            24430..25149
                     /gene="trmB"
                     /locus_tag="ACHKA1_000778"
     CDS             24430..25149
                     /gene="trmB"
                     /locus_tag="ACHKA1_000778"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO5678477.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            25149..25475
                     /gene="yggL"
                     /locus_tag="ACHKA1_000779"
     CDS             25149..25475
                     /gene="yggL"
                     /locus_tag="ACHKA1_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO5678478.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            25659..26378
                     /gene="yggN"
                     /locus_tag="ACHKA1_000780"
     CDS             25659..26378
                     /gene="yggN"
                     /locus_tag="ACHKA1_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO5678479.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            26554..27600
                     /gene="ansB"
                     /locus_tag="ACHKA1_000781"
     CDS             26554..27600
                     /gene="ansB"
                     /locus_tag="ACHKA1_000781"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO5678480.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            27717..28724
                     /gene="yggM"
                     /locus_tag="ACHKA1_000782"
     CDS             27717..28724
                     /gene="yggM"
                     /locus_tag="ACHKA1_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO5678481.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINKACDRWKKDTEQLRRNGVI
                     K"
     gene            complement(28880..30016)
                     /gene="hemW"
                     /locus_tag="ACHKA1_000783"
     CDS             complement(28880..30016)
                     /gene="hemW"
                     /locus_tag="ACHKA1_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO5678482.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAANLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(30009..30602)
                     /gene="rdgB"
                     /locus_tag="ACHKA1_000784"
     CDS             complement(30009..30602)
                     /gene="rdgB"
                     /locus_tag="ACHKA1_000784"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO5678483.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREQKSAISHRGQALKLLLDALRNG"
     gene            complement(30610..30900)
                     /gene="yggU"
                     /locus_tag="ACHKA1_000785"
     CDS             complement(30610..30900)
                     /gene="yggU"
                     /locus_tag="ACHKA1_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO5678484.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(30897..31463)
                     /gene="yggT"
                     /locus_tag="ACHKA1_000786"
     CDS             complement(30897..31463)
                     /gene="yggT"
                     /locus_tag="ACHKA1_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO5678485.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(31481..32185)
                     /gene="yggS"
                     /locus_tag="ACHKA1_000787"
     CDS             complement(31481..32185)
                     /gene="yggS"
                     /locus_tag="ACHKA1_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO5678486.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            32203..33183
                     /gene="yggR"
                     /locus_tag="ACHKA1_000788"
     CDS             32203..33183
                     /gene="yggR"
                     /locus_tag="ACHKA1_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO5678487.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLTENQRLRGSAFAQRQGISLALRLLPSHYPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(33367..33783)
                     /gene="ruvX"
                     /locus_tag="ACHKA1_000789"
     CDS             complement(33367..33783)
                     /gene="ruvX"
                     /locus_tag="ACHKA1_000789"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO5678488.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(33783..34346)
                     /gene="yqgE"
                     /locus_tag="ACHKA1_000790"
     CDS             complement(33783..34346)
                     /gene="yqgE"
                     /locus_tag="ACHKA1_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO5678489.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(34455..35405)
                     /gene="gshB"
                     /locus_tag="ACHKA1_000791"
     CDS             complement(34455..35405)
                     /gene="gshB"
                     /locus_tag="ACHKA1_000791"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO5678490.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(35418..36149)
                     /gene="rsmE"
                     /locus_tag="ACHKA1_000792"
     CDS             complement(35418..36149)
                     /gene="rsmE"
                     /locus_tag="ACHKA1_000792"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO5678491.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(36229..36936)
                     /gene="endA"
                     /locus_tag="ACHKA1_000793"
     CDS             complement(36229..36936)
                     /gene="endA"
                     /locus_tag="ACHKA1_000793"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO5678492.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(37031..37528)
                     /gene="yggI"
                     /locus_tag="ACHKA1_000794"
     CDS             complement(37031..37528)
                     /gene="yggI"
                     /locus_tag="ACHKA1_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO5678493.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(37605..38999)
                     /gene="galP"
                     /locus_tag="ACHKA1_000795"
     CDS             complement(37605..38999)
                     /gene="galP"
                     /locus_tag="ACHKA1_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO5678494.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(39436..40590)
                     /gene="metK"
                     /locus_tag="ACHKA1_000796"
     CDS             complement(39436..40590)
                     /gene="metK"
                     /locus_tag="ACHKA1_000796"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO5678495.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            40646..40897
                     /gene="yqgD"
                     /locus_tag="ACHKA1_000797"
     CDS             40646..40897
                     /gene="yqgD"
                     /locus_tag="ACHKA1_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO5678496.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            41008..41151
                     /gene="yqgG"
                     /locus_tag="ACHKA1_000798"
     CDS             41008..41151
                     /gene="yqgG"
                     /locus_tag="ACHKA1_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO5678497.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            41245..41376
                     /gene="yqgB"
                     /locus_tag="ACHKA1_000799"
     CDS             41245..41376
                     /gene="yqgB"
                     /locus_tag="ACHKA1_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO5678498.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            41385..43361
                     /gene="speA"
                     /locus_tag="ACHKA1_000800"
     CDS             41385..43361
                     /gene="speA"
                     /locus_tag="ACHKA1_000800"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO5678499.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            43507..44238
                     /locus_tag="ACHKA1_000801"
     CDS             43507..44238
                     /locus_tag="ACHKA1_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678500.1"
                     /translation="MKKWKVRSALIALVVLLVGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRA
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVVDSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            44374..45294
                     /gene="speB"
                     /locus_tag="ACHKA1_000802"
     CDS             44374..45294
                     /gene="speB"
                     /locus_tag="ACHKA1_000802"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO5678501.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(45500..46258)
                     /gene="loiP"
                     /locus_tag="ACHKA1_000803"
                     /gene_synonym="yggG"
     CDS             complement(45500..46258)
                     /gene="loiP"
                     /locus_tag="ACHKA1_000803"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO5678502.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            46536..48527
                     /gene="tkt"
                     /locus_tag="ACHKA1_000804"
     CDS             46536..48527
                     /gene="tkt"
                     /locus_tag="ACHKA1_000804"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO5678503.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            complement(48747..49655)
                     /locus_tag="ACHKA1_000805"
     CDS             complement(48747..49655)
                     /locus_tag="ACHKA1_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFO5678504.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVLLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            50033..50476
                     /gene="cmtB"
                     /locus_tag="ACHKA1_000806"
     CDS             50033..50476
                     /gene="cmtB"
                     /locus_tag="ACHKA1_000806"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO5678505.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSATDADSHIGAIQALSELLCEEEILEQLLTASSEKKLADIISRG"
     gene            50504..51892
                     /gene="cmtA"
                     /locus_tag="ACHKA1_000807"
     CDS             50504..51892
                     /gene="cmtA"
                     /locus_tag="ACHKA1_000807"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO5678506.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            51907..53184
                     /gene="yggP"
                     /locus_tag="ACHKA1_000808"
     CDS             51907..53184
                     /gene="yggP"
                     /locus_tag="ACHKA1_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO5678507.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            53181..54146
                     /gene="glpX"
                     /locus_tag="ACHKA1_000809"
     CDS             53181..54146
                     /gene="glpX"
                     /locus_tag="ACHKA1_000809"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO5678508.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVEVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            54168..54677
                     /gene="fumE"
                     /locus_tag="ACHKA1_000810"
     CDS             54168..54677
                     /gene="fumE"
                     /locus_tag="ACHKA1_000810"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO5678509.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMNTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLKEQD
                     ETLGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            54674..55387
                     /locus_tag="ACHKA1_000811"
     CDS             54674..55387
                     /locus_tag="ACHKA1_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO5678510.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIKRVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(55359..56036)
                     /locus_tag="ACHKA1_000812"
     CDS             complement(55359..56036)
                     /locus_tag="ACHKA1_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5678511.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     QPDDIHP"
     gene            complement(56030..56707)
                     /locus_tag="ACHKA1_000813"
     CDS             complement(56030..56707)
                     /locus_tag="ACHKA1_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO5678512.1"
                     /translation="MVTLEQFRYCPTHTTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPHASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(56695..57402)
                     /locus_tag="ACHKA1_000814"
     CDS             complement(56695..57402)
                     /locus_tag="ACHKA1_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO5678513.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLH"
     gene            complement(57403..57981)
                     /locus_tag="ACHKA1_000815"
     CDS             complement(57403..57981)
                     /locus_tag="ACHKA1_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO5678514.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPICTTRHFPAMAAVR"
     gene            complement(58004..58435)
                     /locus_tag="ACHKA1_000816"
     CDS             complement(58004..58435)
                     /locus_tag="ACHKA1_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO5678515.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            58807..59826
                     /gene="epd"
                     /locus_tag="ACHKA1_000817"
     CDS             58807..59826
                     /gene="epd"
                     /locus_tag="ACHKA1_000817"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO5678516.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRSEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            59876..61039
                     /gene="pgk"
                     /locus_tag="ACHKA1_000818"
     CDS             59876..61039
                     /gene="pgk"
                     /locus_tag="ACHKA1_000818"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO5678517.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            61254..62333
                     /gene="fbaA"
                     /locus_tag="ACHKA1_000819"
     CDS             61254..62333
                     /gene="fbaA"
                     /locus_tag="ACHKA1_000819"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO5678518.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(62397..62495)
                     /locus_tag="ACHKA1_000820"
                     /pseudo
     CDS             complement(62397..62495)
                     /locus_tag="ACHKA1_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            62691..63551
                     /gene="mscS"
                     /locus_tag="ACHKA1_000821"
     CDS             62691..63551
                     /gene="mscS"
                     /locus_tag="ACHKA1_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO5678519.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            63690..64325
                     /gene="argO"
                     /locus_tag="ACHKA1_000822"
     CDS             63690..64325
                     /gene="argO"
                     /locus_tag="ACHKA1_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO5678520.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            64418..65158
                     /gene="yggE"
                     /locus_tag="ACHKA1_000823"
     CDS             64418..65158
                     /gene="yggE"
                     /locus_tag="ACHKA1_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO5678521.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            65326..66222
                     /gene="ygfI"
                     /locus_tag="ACHKA1_000824"
     CDS             65326..66222
                     /gene="ygfI"
                     /locus_tag="ACHKA1_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5678522.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(66219..67697)
                     /gene="scpC"
                     /locus_tag="ACHKA1_000825"
     CDS             complement(66219..67697)
                     /gene="scpC"
                     /locus_tag="ACHKA1_000825"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO5678523.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(67721..68506)
                     /gene="scpB"
                     /locus_tag="ACHKA1_000826"
     CDS             complement(67721..68506)
                     /gene="scpB"
                     /locus_tag="ACHKA1_000826"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO5678524.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(68517..69512)
                     /gene="meaB"
                     /locus_tag="ACHKA1_000827"
     CDS             complement(68517..69512)
                     /gene="meaB"
                     /locus_tag="ACHKA1_000827"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO5678525.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIRDGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAETAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(69505..71649)
                     /gene="scpA"
                     /locus_tag="ACHKA1_000828"
     CDS             complement(69505..71649)
                     /gene="scpA"
                     /locus_tag="ACHKA1_000828"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO5678526.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARVIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(71853..72746)
                     /gene="argP"
                     /locus_tag="ACHKA1_000829"
     CDS             complement(71853..72746)
                     /gene="argP"
                     /locus_tag="ACHKA1_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO5678527.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            complement(72817..74142)
                     /locus_tag="ACHKA1_000830"
     CDS             complement(72817..74142)
                     /locus_tag="ACHKA1_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HFO5678528.1"
                     /translation="MKKANLTGTLVCFVACIIFLSSSDIFREKPIALIWFIRQNTLFF
                     TGTLAWCLMTLTMCLSLRSSWLNKVLGGLDKAWRLHKWAGICAIAFAFAHWLDEKLPQ
                     LFVAFGWLTHPGKIVDINLTPVQENWLHAGLLAGECAMFIMIAMVFVSLSKKVPYHLF
                     HLVHRLFPVFYLAIAFHVFTALFKSYWWETPAAYLLILITIPGVFVAFISLLKLNGNK
                     SKHQATVKSIVNHPGQITEVILEMEHPIDYCPGQFAFLTFAHSKESHPFTIASYVKEK
                     NTLRFAIKNLGDYTSTLASSIQIGQSALVEGPWGKFDFTLPCSRQVWIAGGIGITPFI
                     AQLEYLNQHGASRVPVDLWYCVSRSEDAWYVDKLTSLCAQASVTLHLLDAHKGERLQS
                     HYLTDKIANKGDTHFWFCGPQSFAKALSKGLYENGIASQNFHYDRFCMR"
     gene            complement(74204..74464)
                     /locus_tag="ACHKA1_000831"
     CDS             complement(74204..74464)
                     /locus_tag="ACHKA1_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFO5678529.1"
                     /translation="MSIKQNLFAALFLCTLTGHAFAATEIHKPDNNLEQLSKVSMSTH
                     NSNPAGVESQIKEKAEELQASNYYISSFSGKNYIHATVIFYK"
     gene            complement(74481..75110)
                     /locus_tag="ACHKA1_000832"
     CDS             complement(74481..75110)
                     /locus_tag="ACHKA1_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFO5678530.1"
                     /translation="MKILHIDSSPMLKHSSSRKLSQTLVEALCQHFPKATIIHRDVGL
                     TPPPHLREETFMALCDKAECGSAQIRQEVNGIHAAIEELASCDVLIIGAPMINHSVSS
                     GLKTWIDQVCQAGLTFRFTSAGAVGLLTDKPTFIVSTRGGIYCDEAHLALDHQESYLQ
                     ATLKLMGITNTHILRAEGVDKTQPGRELAEQNALAQIPELITQVFNHYL"
     gene            75211..76107
                     /locus_tag="ACHKA1_000833"
     CDS             75211..76107
                     /locus_tag="ACHKA1_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5678531.1"
                     /translation="MIDWNDYYYFALIADEGSYSQAALRAGVSKSVLSRRMAALEERL
                     GVRLIQRTTRRLALTAAGEQFAVECRALMAQEERARAVVMAAQESPQGMLRVSAPVFM
                     AETWFGEFVSEFIARWPLAKVQLLAINRPVDMVAEGIDIALVTASGALADSTLHYKKL
                     HTQADILVASSQWLARHPQVKAIEDLNHVDGILRLANNGGSQWQLINGEQEITLQPEA
                     RLQSNNLRVMLQAVLGGNGVALLPQFACKQGLANGELVRLFAPWSGIPRPLYALFAGR
                     KGMPAIARYFMDELTTRLANGV"
     gene            76163..76822
                     /gene="rpiA"
                     /locus_tag="ACHKA1_000834"
     CDS             76163..76822
                     /gene="rpiA"
                     /locus_tag="ACHKA1_000834"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO5678532.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            77078..78310
                     /gene="serA"
                     /locus_tag="ACHKA1_000835"
     CDS             77078..78310
                     /gene="serA"
                     /locus_tag="ACHKA1_000835"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO5678533.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
BASE COUNT        19068 a        19593 c        21077 g        18717 t
ORIGIN      
        1 cgaatttttt aacattgttt tgtcacttgc gttattaatg aataagaaat ttaaatataa
       61 gaaatcgctt ttagcggcta ttttgagtgc aaccctgtta gccggttgtg atggcggtgg
      121 ctccggatct tcctccgata cgccgcctgt agattctgga acagggtctt tgccggaagt
      181 gaaacctgat ccaacaccaa acccggagcc gacgcctgag ccaacgccgg acccagagcc
      241 tacgccagaa ccgatacctg atcctgaacc aacaccagaa ccggagccag aacctgttcc
      301 tacgaaaacg ggttatctga ccctgggcgg aagccagcgg gtaactggtg ctacctgtaa
      361 tggtgaatcc agcgatggct ttacatttaa acctggcgag gacgttactt gcgtggcggg
      421 taacacgaca attgccacct tcaacactca gtcagaagct gcgcgtagct tgcgtgcggt
      481 tgaaaaagtg tcgtttagcc ttgaggacgc gcaagaactg gcgggctccg atgacaagaa
      541 aagcaatgcg gtttcgctgg taacgtccag taacagctgt ccggcgaata cagaacaggt
      601 ttgtctgacg ttctcctcgg tgatcgagag taaacgcttc gactcgctgt ataagcaaat
      661 cgatctggca ccggaagagt tcaaaaagct ggtcaatgaa gaggtggaaa acaatgctgc
      721 gaccgataaa gcgccatcca ctcatacttc accggtcgtg cccgtcacca cgccgggaac
      781 aaaaccggat ctgaacgctt ccttcgtgtc ggctaacgcg gaacagtttt atcagtatca
      841 accctcggaa atcattctct cagaaggtcg cctggtcgat agccagggat atggtgttgc
      901 tggcgtcaac tactacacca attcaggccg tggcgtgaca ggggaaaatg gtgaattttc
      961 ctttagctgg ggcgaaacca tctcctttgg tatcgatacc tttgaactgg gttcagtgcg
     1021 cggcaataag tcgaccattg cgctgactga actgggtgat gaagttcgcg gggcgaatat
     1081 tgatcagctt attcatcgct attcgacgac cgggcaaaat aatacccgtg ttgttccgga
     1141 cgatgtacgc aaggtctttg ccgaatatcc caacgtgatc aacgagatta tcaatctctc
     1201 gttatccaac ggtgcgacgc tgggggaagg tgagcaagtt gttaatctgc caaacgaatt
     1261 tattgagcag tttaatacgg gtcaggccaa agagatcgat accgcgattt gtgcgaaaac
     1321 cgatggttgt aacgaggctc gctggttctc gctgacgacg cgcaatgtta atgacggcca
     1381 gattcagggc gttattaaca agctgtgggg tgtggatgaa gattacaaat cggtgacgaa
     1441 attccacgtc tttcatgact ctaccaactt ctatggcagc actggtaatg cgcgcggtca
     1501 ggcggtggtg aatatctcca acgcggcctt cccaattctg atggcgcgta atgataaaaa
     1561 ctactggctg gcgtttggcg aaaaacgcgc ctgggataaa aacgagctgg cgtacattac
     1621 ggaagcgcct tctcttgttg agccggaaaa cgttacgcgc gataccgcca cctttaacct
     1681 gccgtttatt tcgctggggc aagtcggtga gggcaaactg atggttatcg gtaacccgca
     1741 ctacaacagc attttgcgtt gcccgaacgg ttacagctgg aacgggggcg ttaataaaga
     1801 cgggcagtgt acgctcagcg gtgattcaga tgacatgaag cacttcatgc agaacgtact
     1861 gcgctatctg tccgacgata aatggacgcc ggacgcgaaa gccagcatga ccgtaggcac
     1921 caacctggat actgtctatt tcaaacgtca tggtcaggtt acaggaaaca gcgctgagtt
     1981 cggctttcat ccggattttg cgggtatctc tgttgagcat ttaagtagct atggcgatct
     2041 cgacccgcag gaaatgccgc tgctgatcct caacggcttt gagtatgtga ctcaggttgg
     2101 taatgatcct tatgcaatcc cgctgcgtgc agataccagc aaaccgaagc tgactcagca
     2161 ggatgtgacc gatttgatcg cctatatgaa caaaggtgga tcggtgctga tcatggaaaa
     2221 cgtgatgagc aatcttaagg aagagagcgc atctggcttt gtacgtctgc ttgatgccgc
     2281 aggtttgtcg atggcgctta acaagtcggt agtaaataac gatccgcaag gctacccgga
     2341 ccgcgttcgt caacgacgtt caacgccaat ttgggtctat gagcgttatc cggctgtcga
     2401 tggtaaacca ccgtatacca ttgatgacac cacgaaagaa gttatctgga aatatcagca
     2461 agaaaacaaa cctgatgaca aaccgaagct ggaagttgcc agctggcagg aagaagttga
     2521 gggtaaacag gtaactcaat tcgcctttat cgatgaagcc gaccacaaaa cgcctgagtc
     2581 actggctgcg gcgaagaaga gaattctgga cgcgttccca gggctggaag agtgtaagga
     2641 ttctgactac cactacgagg tcaactgcct ggaatatcgt cctggcacgg gagttccggt
     2701 tactggtggc atgtatgttc cacagtatac gcaactaagc cttaacgccg acactgcaaa
     2761 agcgatggtg caggctgcag atttaggcac caacattcag cgtctgtatc agcatgagct
     2821 ctacttccgg accaatggtc gcaaaggtga gcgtctgagc agcgtcgatc tggaacgtct
     2881 gtaccaaaac atgtcggtct ggctgtggaa cgatacgagc tatcgttatg aagaaggcaa
     2941 aaatgacgag ctgggcttta aaacgttcac cgagttcctg aactgctata ccaatgatgc
     3001 ctataccgat ggcacacagt gctccgcaga tctgaaaaaa tcgctggtcg ataacaacat
     3061 gatctacggt gaaagtagcg gtaaagcggg catgatgaac ccaagctatc cactcaacta
     3121 tatggaaaaa ccgctgacgc gcctgatgct gggccgttcc tggtgggatc tgaacattaa
     3181 ggttgatgtg gagaagtatc cgggagcagt atcggaagag ggacagaacg ttactgaaac
     3241 catcagcctg tactcgaatc cgaccaaatg gtttgcgggt aacatgcaat caaccggcct
     3301 gtgggcaccg gctcagaaag aggtcaccat taagtccaat gcgaacgttc ctgtgaccgt
     3361 caccgtggcg ctggctgacg acctgaccgg acgtgagaag catgaagttg cgctgaaccg
     3421 tccgccaaga gtgactaaaa cgtactctct ggacgctagc ggtacggtga agttcaaggt
     3481 gccttacggt ggcctgattt atatcaaggg caatagctct accaatgaat ctgccagctt
     3541 cacctttact ggcgtggtaa aagcaccgtt ctataaagac ggcgcatgga aaaacgatct
     3601 gaactcaccg gctccgctgg gcgaactgga gtctgcctcg ttcgtctata ccacaccgaa
     3661 gaagaacctg aatgccagca attacactgg cggactggag caattcgcta acgatctgga
     3721 cacctttgcc agctcgatga atgacttcta cggtcgtgat gaaacgagcg gtaagcaccg
     3781 gatgtttacc tataaagcat tgacgggaca taaacatcgt ttcacaaacg atgtgcagat
     3841 ctccatcggt gatgcgcact cgggttatcc ggtaatgaac agcagcttct cgacgaacag
     3901 caccacgctg ccgacgacgc cgctgaacga ctggttgatc tggcacgaag tagggcacaa
     3961 cgccgcagaa acgccgctga ctgttccagg cgcgaccgaa gtggcgaaca acgtgctggc
     4021 gctgtacatg caggatcgct atctcggcaa gatgaaccgt gtcgctgacg atattaccgt
     4081 cgcgccggaa tatctggagg agagcaacaa ccaggcttgg gcgcgcggcg gtgcgggtga
     4141 ccgtttgctg atgtacgcgc agctgaagga atgggcagag aaaaactttg atatcaagaa
     4201 atggtatcca gatggcactc ctctgccaga gttttacagc gagcgtgaag ggatgaaagg
     4261 ctggaacctg ttccagttga tgcatcgtaa agcacgcggc gatgatgttg gcaatgacaa
     4321 atttggcaac agaaactact gtgccgaatc caacggtaac gctgccgaca agctgatgct
     4381 gtgtgcctcc tgggtcgccc agacggatct ttcggagttc tttaaaaaat ggaatccggg
     4441 cgcgaatgct taccagctgc cgggggtgag cgagatgaac ttcgagggcg gtgtgagcca
     4501 gtcggcttac gagacgctgg cggcgcttgg tctgccgaaa ccgcagcaag ggccggaaac
     4561 cattaatcag gttaccgagc ataagatgtc agccgagtaa gcctgtatgc cggataaggc
     4621 gctcgcgccg catccggcat gaaataaggc gcaccgggtc aacacatatg cccgatgcgc
     4681 aagcttatcg ggcctacaga gagggaaagc tgcgtttatt tgcgttggca gacacctgta
     4741 ggccggataa ggcgctcgcg ccgcatccgg catgaaataa ggcgcatcgg gttaacacat
     4801 atgcccgatg cgccatctta tcgggtcttc agggaaagcc gcgattactt gcgttggcag
     4861 atacctgtag gccggataag gcgttttacg ccgcatccgg caagttacaa caaacaacct
     4921 ttaaccatgc tttttgatgt ttttcagcaa taccccgtgg cgatgcccat actggctacc
     4981 gtcggaggat tgatcatcgg cagttttttg aatgtggtga tttggcgtta ccccatcatg
     5041 ctgcgccaac aaatggcgga gtttcatggt gaaatgccga gtgcgcagtc aaaaataagc
     5101 cttgcgctac cgcgttcgca ctgcccacat tgtcagcaga ccatccggat acgtgacaat
     5161 attccgctgc tctcctggct gatgctcaaa gggcgctgcc gtgattgtca ggcgaaaatc
     5221 agcaagcgtt atccgctggt ggagttattg acggcactcg cttttttgct ggcgagcctg
     5281 gtgtggccgg aaagtggatg ggcgctggcg gtgatgatat tatccgcctg gctgattgcc
     5341 gcgagcatca tcgacctcga tcaccaatgg ctgcccgatg tttttactca gggcgtattg
     5401 tggacgggac taattgcggc atgggcgcag cagagcccgc tcacgctaca agatgcagtc
     5461 accggcgtcc tggtggggtt tatcactttt tactctctgc gctggatagc cggaatagtt
     5521 ctgcgtaaag aagcattagg catgggcgat gtattattgt tcgccgcttt aggtagttgg
     5581 gtgggaccgt tatcactgcc caatgttgct ttaatcgcct catgctgcgg cctgatatat
     5641 gccgttatta caaaaagagg atcaaccaca ctgccttttg gaccgtgttt aagtctgggc
     5701 ggtatagcaa cactttatct acaggcattg ttttaatgat aaccacgtca ttatcaaagt
     5761 gacattttaa ctcttattaa taaccttaga gattatttac catgtcgata aaacaaatgc
     5821 cagggagggt attaatatcg ctattgttga gcgttacagg attattaagt ggctgtgccg
     5881 gccataatga aaatgccagt ttactggcga aaaaacaggc gcaaaatatc agccaaaacc
     5941 tgccgattaa atctgcggga tataccttag tgctggcaca aagtagcggc acgacggtaa
     6001 aaatgaccat tatcagcgaa gcgggtactc agatcacgca gacgccagac gcctttttaa
     6061 ccagctatca acgacaaatg tgcgctgacc caacggtgaa attaatgctc actgagggaa
     6121 ttaattacag cataacgatt aatgatacac gtacaggtaa ccagtatcag cggaaactgg
     6181 atcgtaccac ctgtggaata gtcaaagcat aacgtcgggt agatataaat tggcgcgggt
     6241 tgtttttcgt gacgcacgaa tttatctcat tcaatggctg acaaaaattc gtcacactct
     6301 taaccagaga caatctctta atacagacaa agagcatctg cgaaaaattg cacgcgggat
     6361 gttctggctg atgctgctta ttatttctgc aaaagtggcg cattcactct ggcgttattt
     6421 ctccttttct gcggaatata cggtggtttc tccatcggtg aataaaccgc cccgtacgga
     6481 tgcaaaaacg ttcgataaaa atgacgtgca attaatcagc cagcaaaact ggtttggcaa
     6541 atatcagccc gtcgccgcac cggtaaaaca acccgaacct gcacctgtgg ccgaaacgcg
     6601 tcttaatgtg gtgctgcgcg ggatcgcctt tggtgccaga cccggcgcgg ttattgaaga
     6661 aggtggcaaa cagcaggtct atttgcaggg ggaacggctt ggttctcaca acgcggtgat
     6721 tgaggaaatc aaccgcgacc atgtgatgct gcgctatcag ggaaaaatag agcgcctgag
     6781 cctggttgaa gaggagcgtt ccaccgtagc cgtgaccaac aaaaaagcca tcagtgacga
     6841 agcaaagcaa gctgttgctg aacctgctgt cagtgcgcca gttgagatcc cgactgccgt
     6901 gcgtcaggca ctggcgaaag atccgcagaa aatttttaac tatatccagc ttacgcctgt
     6961 gcgtaaggag ggaattgtcg gttatgcagt gaaaccgggg gcagatcgtt ctctgttcga
     7021 tgccagcggt ttcaaggaag gcgatatcgc cattgcgctc aatcagcagg atttcactga
     7081 tccacgagca atgattgctc tgatgcggca gttaccttca atggattcca ttcaacttac
     7141 ggttttacgc aagggtgcgc gccacgacat ttccatcgcg ctgcgctaac cgcatttaat
     7201 ccaggagaat cattcatcgt gttttggcgt gatattacgt tgtcggtctg gcgtaagaag
     7261 acaactggcc tcaaaacaaa aaagcgttta ctgccgctgg tgttggcagc ggcattatgc
     7321 agttcaccgg tctgggcgga agaagccact ttcaccgcta attttaaaga taccgacctg
     7381 aaatcgttca tcgaaaccgt cggcgctaac cttaataaaa ccatcattat ggggccgggc
     7441 gttcagggga aagtgagtat tcgcactatg actccgctca atgaacgcca gtattaccag
     7501 ttattcctta acctgctgga agcacagggg tatgcggtcg taccgatgga aaacgacgtg
     7561 ctgaaggtgg tgaaatccag tgccgcgaaa gtcgagccgc tgccgctggt tggtgaaggc
     7621 agcgacaact acgcgggcga tgaaatggtc accaaagtag tgccggtacg taatgtgtcg
     7681 gtacgcgagc tggcaccgat tctgcgccag atgatcgaca gcgcaggctc aggcaacgtt
     7741 gttaattacg atccctccaa cgtgattatg ctcaccggac gcgcctccgt cgtggagcgg
     7801 ctgacggaag tgatccagcg tgtggatcac gcgggtaatc gcactgaaga ggtgatcccg
     7861 ctggataacg cctcggcttc ggaaatcgcc cgcgtgctgg aaagcctgac gaaaaacagc
     7921 ggcgagaacc agcctgcgac gctgaaatct caaattgtcg ccgacgaacg caccaacagt
     7981 gtgattgtca gtggtgaccc ggccacgcgg gacaaaatgc gccgcctgat ccgtcggctg
     8041 gactcagaaa tggagcgcag cggcaacagc caggttttct atctcaaata cagcaaagcc
     8101 gaagatctgg tcgatgtact gaagcaggtc agcggcacgc tcacggcggc taaagaagag
     8161 gcggaaggca cggtcggtag cgggcgtgag gttgtctcca tcgccgccag caaacacagt
     8221 aatgccctga ttgtaaccgc gccgcaggac atcatgcagt cgctgcaaag cgtgattgaa
     8281 caactggata ttcgccgtgc tcaggtgcac gtcgaggcgt tgatcgtgga agtggccgaa
     8341 ggcagtaata tcaacttcgg cgtgcagtgg gcgtcgaaag atgccggatt aatgcagttt
     8401 gctaatggta cgcagatccc tatcggtacg ctgggcgcag ccatttctca ggcgaaaccg
     8461 cagaaaggct cgacggtgat cagtgaaaac ggcgcaacca ccattaatcc ggataccaac
     8521 ggcgatctct ccacgctcgc ccagcttctt tccggcttta gcggtacggc ggttggcgtg
     8581 gtgaaaggcg actggatggc gctggtacag gcggttaaaa acgactccag ctcgaacgtt
     8641 ctctccacgc cgagcatcac cacgctggac aaccaggaag ccttcttcat ggtgggtcag
     8701 gacgttccgg tattaaccgg ctctaccgtt ggctccaata acagcaatcc tttcaataca
     8761 gtagagagga aaaaagtcgg catcatgctg aaagtcacgc cgcagattaa cgaaggtaac
     8821 gcggtacaga tggtgattga gcaggaagtc tcgaaggtgg aaggacagac cagcctcgac
     8881 gtggtgtttg gtgagcgcaa actgaagacc accgtgctgg ctaacgatgg tgaactgatc
     8941 gtgcttggcg gtctgatgga cgaccaggcg ggagaaagtg tggcgaaagt gccgctgctg
     9001 ggcgatatcc cgttgattgg taacctgttt aaatcgacgg cggataaaaa agaaaaacgt
     9061 aacctgatgg tgtttatccg tccgaccatt ctgcgtgacg gtatggcggc agacggcgtg
     9121 tcgcagcgca aatataacta tatgcgcgcc gaacagatct atcgcgatga gcaaggctta
     9181 agcctgatgc cgcacaccgc gcagccggta ctgccagcgc aaaaccaggc cttaccgccg
     9241 gaagtacgtg cgttccttaa tgccgggaga acgcgttaat ggtgcctgta gcacaggaaa
     9301 ccaccgctaa caccgtgcgt ctgccctaca gtttcagccg tcggtttagc ctggtggcat
     9361 ggtgcgaagc gtcgctggag atcctccatg tgcatccgtt gtcgctctct gttttgcagg
     9421 agctgcagcg ggggctgaac gcgcccttta cgctgcggca aatcgacgag gccgaatttg
     9481 aacagcgact gaatgcggtc tggcagcggg actcttccga ggcccgccag ctaatggaag
     9541 atctcggttc tgctgaggac ttttttactc tcgctgaaga actgccggaa acggaagatc
     9601 tgctggaaag tgacgacgat gcgccgatca tcaaactgat caacgccatg ctggcagagg
     9661 cgattaaaga aggcgcttcg gatattcaca tcgagacgtt tgaaaagagt ctggtgatcc
     9721 gttttcgcgt tgacggcaca ttacatgaaa tgctgcgccc ggggcgcaaa ctggcctcgc
     9781 tgctggtatc gcgtatcaag gtgatggcac ggctggatat cgccgaaaag cgcgtaccgc
     9841 aggatggacg tattgcgctg ctgctgggcg gtcgggcgat tgacgtgcgt gtctccacca
     9901 tgccctccgc ctggggcgag cgcgtggtgc tgcgactgct ggacaaaaac caggcccgcc
     9961 tgacgctgga gcgtctgggg cttagccagc aactgaccgc gcagttgcgc cagctgttac
    10021 acaaaccgca cggcatcttt ctggtaacgg ggccgacggg ttccggaaaa agcaccacgc
    10081 tgtacgccgg attgcaggag ctgaacaacc attcgcgcaa tattctcacg gttgaagacc
    10141 ccatcgaata catgattgaa ggaattggtc agacacaggt taacacccgc gtcggcatga
    10201 cctttgcccg tggcctgcgc gcgattttgc gtcaggaccc ggatgtggtg atggtcggtg
    10261 aaatccgcga taccgaaacc gcagaaatcg ccgtccaggc ttcacttacc ggacacctgg
    10321 tcctttccac gctgcatacc aacacggcgg tgggggcgat cacgcgtttg caggatatgg
    10381 gcgtggagcc tttcctgctc tcttccagtc tgacgggcgt gatggcgcag cgactggttc
    10441 gcacgctgtg ccccgactgc cgccagcccg cgcctgccac tgacgaagaa aaacgcctgc
    10501 tgggaattac cgacgcgcat accgtcactc tgtaccatcc gcagggctgc cccgcctgta
    10561 atcataaagg tttccgcgga cgtactgcca tccatgagct gatcgtggtg gatgccacat
    10621 tgcgtgattt gatccaccgt caggccgggg aactggagct ggaacgttat gtccggcaac
    10681 actctgcggg catccgcagc aacggcattg agaaagtgct cgccggagaa acctctctcg
    10741 atgaagttct gcgggtaacc atggaggcgt aatggcactg ttttactatc aggcgctgga
    10801 gcgtaatggt cgcaaaacca aaggcatgat tgaggcggat tccgcgcgtc atgcccgcca
    10861 gttgttgcgc ggtaaagatc tcattcccat gcacattgaa gcccggatga atgcttcggc
    10921 agggggaatg ttgcagcgtc ggcgacacgc acatcgtcgc gtggcgtcgg cagatctggc
    10981 gctgttcacg cgccaactgg caacgctggt gcaggcagca atgccgctgg aaacctgctt
    11041 acaggcggtc agtgagcaaa gtgaaaaact gcatgtaaaa agcctcggaa tggcgctgcg
    11101 cagccggatt caggaaggtt acaccctgtc ggacagcctg cgcgaacatc cccgcgtctt
    11161 tgattccctg ttttgttcga tggtggctgc cggagaaaaa tccgggcatc tcgacgtggt
    11221 gcttaatcgc ctggcggatt acaccgaaca gcgacagcgc ctgaaatcac gtctattgca
    11281 ggccatgctc tatccgctgg ttctgctagt ggtggcaacg ggcgtggtca ctattttgct
    11341 gacggcagtg gtgccgaaaa tcatcgaaca gttcgatcac ctcggacacg cgctgcccgc
    11401 caccacccgc gcgcttatcg ccatgagtga cgcgttacag gccagcggcg tttactggct
    11461 ggcgggattg ctggcgcttc tggtgctggg gcaacggcta ctcaaaaatc ctgttatgcg
    11521 cctgcgctgg gacaaaacct tgctgcgcct gcccgtgaca gggcgtgttg cgcgcggact
    11581 gaatacggcg cggttttccc gcacattaag catcctcacc gccagcagtg ttccgctgct
    11641 ggaaggcatt cagaccgctg ccgctgtgtc ggcaaatcgc tatgtcgagc aacaactgct
    11701 gctggcggca gatcgcgtcc gcgaaggaag cagtctgcgc gccgcgctgg cggagttgcg
    11761 cctgttcccc ccgatgatgc tgtacatgat cgcctccggc gaacagagcg gcgaactgga
    11821 aaccatgctt gagcaggccg ctgtcaacca ggagcgggaa tttgataccc aggtggggct
    11881 ggcgttaggg ctgtttgaac cggcgctggt ggtgatgatg gcgggcgtgg tgctgtttat
    11941 cgtcatcgcc atcctcgagc cgatgctgca actgaacaat atggttggaa tgtaatttac
    12001 ggagttatca catgaattcg ttatcccgca cacaaaaacc acgggcaggt tttaccctgc
    12061 tggaagtgat ggtggtgatt gttattcttg gcgtcctggc aagtctggtg gtgcctaacc
    12121 tgttgggcaa taaagagaaa gcggatcggc aaaaagccat cagcgatatc gtggcgctgg
    12181 aaaacgcact ggatatgtac cgactggata acgggcgtta tccaactacc gagcaggggc
    12241 ttgaggcgct gatccagcag ccagccaata tggcggacgc ccgcaactac cgtactggtg
    12301 gatacattaa acgactgcca aaggatccgt ggggcaatga ttatcagtat ctcagcccgg
    12361 gtgaaaaagg gctgtttgat gtttataccc tgggggcgga tggtcaggaa aacggggaag
    12421 gcgctggcgc agatatcggt aactggaatt tgcaggagtt tcagtaagca gtgcctgaac
    12481 gcggattcac acttctggaa atcatgctgg tgattttcct tatcggcctt gccagtgcgg
    12541 gcgtggtaca gacttttgcg accgattctg aatcgcccgc gaaaaaagcg gcgcaggatt
    12601 ttctgactcg ctttgcgcag tttaaggaca gggcagtgat cgaagggcaa acacttggtg
    12661 tgttaatcga cccgccggga taccagttta tgcaacgtcg tcaggggcag tggttgcccg
    12721 tttctgcgac ccggttatcg gcacaggtta cggtgccgaa acaggtgcag atgctgttac
    12781 aacccggaag tgatatctgg cagaaggaat atgcgctgga gctgcaacgt cgtcgcctga
    12841 cgctgcacga tattgaactg gagttgcaaa aagaggcgaa aaagaagacg ccgcagatcc
    12901 gtttttcgcc ttttgaaccc gccacgccgt ttacgctgcg cttctactca gcggcgcaaa
    12961 acgcatgttg ggcggtaaaa ctggcacacg atggcgcgtt atccctcagt caatgtgatg
    13021 agaggatgcc atgaagcgcg gatttacctt gctggaagtg atgctcgcac tggcgatttt
    13081 tgcgctggct gccacggcgg tgttacagat tgccagcggt gcgctgagta atcagcacgt
    13141 tcttgaggag aaaacggtag caggctgggt ggctgaaaac cagaccgcgc tgctctacct
    13201 gatgacccgt gaacaacggg cggtcaggca tcagggcgag agcgatatgg ctggaagccg
    13261 ctggtactgg cgaaccacac cgcagaatac aggcaacgcg ctgctccagg cggtggatat
    13321 tgaagtcagc cttcacgaag acttttcgtc ggtaattcag tcacgacgcg cctggtttag
    13381 cgccgtggga ggccaacagt gagaagggct cgcgctggtt tcacgttact ggaaatgctg
    13441 gtggcaattg ccatttttgc ctcgctggca ctgatggcgc agcaggtgac taacggcgtc
    13501 acgcgcgtga atagcgccgt caccggacac gatcaaaaac taaacctcat gcagcaaacg
    13561 atgagttttc tgacccacga tctgacccaa atgatgccgc gtccggtaag aggcgatcag
    13621 ggccagcgag aaccagcgtt actggctggc gctggcgtgc tggcatctga gagtgaaggg
    13681 atgcgctttg tgcgtggcgg cgtggttaat ccgttgatgc gtctgccacg cagcaatctg
    13741 ctcaccgtcg gttaccgcat tcatgacggc tatctcgaac ggttagcctg gccgctgacc
    13801 gacgcagcag gcagcgtgaa gccgacaatg caaaagttga ttccggcgga ttcgctccgc
    13861 ttgcagttct atgatggtac gcgctggcag gagaactggt cgtcagtaca ggcgatccct
    13921 gtggcggtgc gcatgaccct gcattcgccg caatggggcg agattgaacg catctggttg
    13981 ttacgcgggc cgcaattatc atgatcacct caccaccaaa acgcggaatg gcactggttg
    14041 tggtgctggt attgctggcg gttatgatgc tggtaaccat cacgctttcc gggcggatgc
    14101 agcaacaact tgggcgaacc cgcagccagc aggagtacca gcaggcgctg tggtacagcg
    14161 ccagtgcaga aagcctggcg ctgagtgcac tcagtctgag cctgaaaaat gaaaagcgcg
    14221 tgcatctggc acagccgtgg gcttccggcc ctcgtttttt cccactgccg caggggcaaa
    14281 tcgccgtcac tctgcgtgac gcacaggcgt gctttaacct gaatgccctc gctcagccga
    14341 cgacggcgtc gcgtccgctc gcgttacaac aactgattgc cctgatctcg cgcctcgatg
    14401 tgcctgctta tcgggccgaa ctgatagccg aaagcctgtg ggagtttatt gatgaagacc
    14461 gcagcgtgca gacgcgtctg ggccgtgaag acagcgaata tctcgcccgc tcggtgcctt
    14521 tctacgccgc caatcaaccg ctggccgata tcagcgagat gcgcgtggtg cagggaatgg
    14581 acgccgggct ttatcaaaaa ctgaaaccgc tggtctgtgc gctgccgatg acccgccagc
    14641 aaatcaacat caatacttta gacgtcacgc aaagtgtgat tcttgaggcg ctgtttgacc
    14701 cgtggttaag ccctgttcag gcgcgggcgt tattacaaca acgtccggcg aagggctggg
    14761 aagatgtcga tcagtttctt gcacagccgc tacttgctga cgtcgatgag cgtactaaaa
    14821 aacagctaaa aaccgtcctg agcgtggaca gcaattactt ctggctgcgt tcagatatca
    14881 ccgtgaatga gattgaactg acgatgaact cgttaattgt ccgcatgggc ccacaacact
    14941 tttctgttct ctggcatcag acaggagaaa gtgagtgagt tccatccttg agattttttt
    15001 cccgctttgc gcctccgaca ccatccgctg gcagcgccgt acacccgacg tggaacacgg
    15061 tatctggcct gacgttgctg acgaacgtct ccagcaatgg ctgcaaactg atgcgattcg
    15121 actctacatt cccggcgaat ggatcagcgt ctggcaggtt gaactgcctg atgtcccacg
    15181 caagcagata ccgactattc tgcccgcctt actggaagaa gagctgaacc aggatatcga
    15241 tgaactgcat ttcgccccgt tgaaaatcga ccagcaactg gcaaccgtgg cagtgattca
    15301 ccaacagcat atgcgcaaca ttgcgcagtg gttgcaggca aacggcatca cccgcgccac
    15361 cgtcgcgcca gactggatgt ccataccttg tggctatatg gctggcgatg cgcaacgggt
    15421 tatctgccgc atcgatgaat gccggggatg gagcgccggg cgggcgctgg ctccggtcat
    15481 gttccgcgcc cagctaaatg agcaggattt accgctttcg ctaaccgtag tcggcattgc
    15541 accggaagag ctatcagcat gggctggcgc agatgctgaa cgcctgaccg ttacggctct
    15601 gcccgccatt accacttatg gcgaaccgga agggaacctg ctaacagggt cgtggcagcc
    15661 tcgcgtcagc taccgaaaac agtgggcgcg ctggcgggtg atgattctgc cgatattgct
    15721 gattctggtt gcgctggcag tggaacgggg cgtgacgtta tggagcgtca gcgaacaggt
    15781 ggcgcaaagc cgcgaccagg cggagaaaca gttcttaacg ttgttcccgg agcagaagcg
    15841 gattgtgaat ttacgctctc aggtgacgat ggcgctgaaa aaatatcgcc cacaggccga
    15901 cgatacccgg ctgctcgccg agttgtcagc gatagccagc accctgaaat cagcgtcact
    15961 ttccgacatc gagatgcgtg gtttcacctt tgatcaaaaa cgccagacgc ttcacctcca
    16021 gttgcgggca gcaaactttg ccagtttcga caaactgcgt agtgcactgg caaccgatta
    16081 tgttgtgcaa caggacgcgt tacagaaaga gggtgatgcg gtttccggcg gcgtaacgtt
    16141 gcggaggaaa taacatgtta cgcgataaat ttattcacta ttttcagcaa tggcgtgaac
    16201 gccagttaag ccgtggcgaa cactggctgg cacaacacct ggcggggcgt tctccgcgtg
    16261 aaaagggcat gttactggca gcggtggtgt tcctgtttag cgtcggatat tacgtcctca
    16321 tctggcagcc gttgagcgaa cggatagaac aacaggagac gatgttgcag caactggtgg
    16381 cgatgaacac ccgactgagg agcgccgcgc cggatattat tgcagcgcga aaatccgcca
    16441 cgacagcacc tgcgcaggta tcgcgggtca tcagtgacag tgcttccgcg cactcggtgg
    16501 tcatcaagcg gatagccgag cgtggggaga atattcaggt ctggatagaa cctgtggtgt
    16561 ttaatgacct tctgaaatgg ttaaacgcac tggatgaaaa atatgcgctg cgggtaacac
    16621 aaattgatgt cagtgctgct gagaagactg ggatggtgaa tgtgcagcgg ctggagtttg
    16681 gacgagggta aagtttcagg agattaatat caataaaatt actctcgtta acgtggcgag
    16741 agtaattccc ccagacttta taagctaatt tttttcgtga aggttattgt cagatccaaa
    16801 agcaaaaacc cgccttgtgg gcgggttctt aagaattagt ggtgcccgga ctcggaatcg
    16861 aaccaaggac acggggattt tcaatcccct gctctaccga ctgagctatc cgggcaacgg
    16921 ggcgcattaa acctgattcg cctcgtctcg tcaatcaaat ttcttcaatt tactgcagac
    16981 tgcacaatct atcatcactt tgccgctatt gcacgcattg tcaggcaaac caggcggtcc
    17041 aggctgcgga aagcggcatt gccagaagga gtgctggaag catgttgacc accgggaaca
    17101 ttttaatgcc acagatgcgt aatccggttg ccagcagcaa taaaccgcct acggcgctga
    17161 agtctgccat catcgacggt gtggtcagcg gtaatatcag cgcggcagcc cacgccagcg
    17221 ttaactggat gatcagtaat gggatactaa ttaccgacac cgcaataccc agcgagcagg
    17281 cgaagatcat cgccgtaaag aaatcaagaa atgacttggc gattaaaata ctcggatcgc
    17341 cggtcatccc ttcgttcatc gccccgaaga tcccggtgcc gctggcgcaa aacaggacaa
    17401 taatcgcgac ataattctga ataaaaggtt catgcgctgg cttcttacgt gagtgacgaa
    17461 acagattttg tgctttggcg accgctgtat tgacgccttt ctccagcaga caaatttcgc
    17521 cgattaatgc tccgagtaag gttgctaaaa ccatcgctgg aaggttggcg catttcacca
    17581 ccagtaaaat accaatcccc agcgatgcca gaccaaaaat tgatgtcatg gagacgcgga
    17641 tacgttccgg taagcgttgg ctaagcagtg caccgagcat gccacccagt aatattgcac
    17701 ttgcgttgat aaaagggccg atgaccacaa gagttcctgt taactgtatt gtttgatttt
    17761 cttattatga actttttgcc ttgttgatgg cttgtctggt cgcaaagtcc tggcttgcac
    17821 gtcttagcga aaggtgccat gattgcgcga attttctcct ctctgtacgg agtttgcccg
    17881 atgcacgcca tctccttaca ttctctcgct tatcgccgtt tcgcgcgaaa cccttctctt
    17941 tttatgctac tgctcatgcg gtgaagttaa cgcacgttca ctgcaggaca acagtaaaat
    18001 cagagcgttt ctgcttttac tgatgtctgg cggtcggagc tggtgaccag tttgacccat
    18061 atctcatggg gcagggtttt acaccttgtc cggtattctt acttccccga aacgggtttt
    18121 gcgcttatga aatcaatgaa tattgccgcc agtagtgaac tggtatcccg actttctact
    18181 catcgccgcg tggtggcgtt gggagatact gattttacgg acgtcgcggc agtcgtcatt
    18241 accgctgcgg atagtcgcag tggcattctt acgttgctta agcgcaccgg ttttcatcta
    18301 ccggtgtttt tgtattccga acatgctgtt gaattacctg cgggcgttac ggcggtaatc
    18361 aacggcaacg agcagcattg gctggagctg gaatccgcag cctgtcagta tgaagagaat
    18421 ttgctgccac cgttttatga cacgctgacg cagtacgttg agatgggcaa cagcaccttt
    18481 gcttgccctg ggcatcaaca tggtgcgttt tttaaaaagc atcctgccgg acgccatttt
    18541 tacgatttct ttggtgagaa cgtctttcgc gccgatatgt gtaatgctga cgtaaaattg
    18601 ggcgatctgc ttattcatga aggatcggcg aaagatgcgc agaaattcgc agccaaagtc
    18661 tttcatgccg ataaaaccta ttttgtactg aacggcacat cggcagcgaa taaagtggtg
    18721 acgaatgcgc tattaacgcg tggcgatctg gtgctcttcg accgtaacaa ccataagtcg
    18781 aatcatcacg gcgcgctgat tcaggcgggg gcgacgccgg tctatctgga agcttcacgc
    18841 aacccgtttg gtttcattgg cggtattgat gcgcactgtt ttaatgaaga gtatctgcgc
    18901 cagcaaattc gcgacgttgc gccagaaaaa gccgacctgc cgcgcccgtt tcgcctggcg
    18961 attattcagc tgggaaccta tgacggcact gtctataacg cccgtcaggt gatcgatacc
    19021 attgggcatc tgtgtgatta cattctgttt gattccgcgt gggtcggtta cgaacagttt
    19081 atcccgatga tggcggatag ctcgccgctg ctgttagaac ttaacgaaaa cgatccaggg
    19141 atctttgtga ctcagtcggt gcacaaacag caggcgggat tctcacagac gtcgcagatc
    19201 cataaaaaag ataaccatat ccgcggacag gcgcgttttt gcccgcataa gcggttgaat
    19261 aacgccttta tgctccatgc ttctaccagc cctttctatc cgctgtttgc tgcactggat
    19321 gttaacgcca aaattcatga aggggagagt gggcgtcggc tgtgggctga gtgcgttgag
    19381 ttgggtattg agtcgcgcaa ggctattctt gcgcgctgta agctgttccg cccgtttatc
    19441 ccgcccgttg ttgatggcaa attgtggcag gattatccga catcagtgtt agccagcgac
    19501 cgccgttttt tcagttttga gccgggggcg aagtggcacg gctttgaagg atatgccgcg
    19561 gatcagtatt ttgttgatcc gtgcaagctg ttactcacta caccaggtat cgatgccgaa
    19621 accggcgagt atagcgactt tggcgttccg gcgacgattc tggcgcacta tctgcgtgag
    19681 aacggcattg tgccggagaa gtgcgatctc aactccattc tgttcttatt aactccggcg
    19741 gaaagccaag agaagctggc acaactggtg gcgatgctgg cgcaatttga acagcatatt
    19801 gaggatgact cgccgctggc tgaggtgttg ccgagcgttt ataacaaata tccggtgcgc
    19861 tatcgcgact acaccctgcg ccagttgtgt caggagatgc acgatctgta tgtcagtttc
    19921 gacgtcaaag acctacaaaa agcgatgttc cgccagcaga gtttcccgtc agtggtgatg
    19981 accccccagg atgcgcatag cgcttatatt cgcggtgacg tggagttggt gcggattcgt
    20041 gatgccgaag ggcgaattgc ggtagaaggg gcgttgcctt atccacctgg cgtgctttgc
    20101 gtggtacccg gggaagtctg gggtggggcg gttcaacgtt atttccttgc actggaagaa
    20161 ggggtgaatt tgttgccggg attttcgccg gagctgcaag gtgtttatag cgaaaccgat
    20221 gcggatggca tgaaacggtt gtacggttat gtgttgaagt aagaataaaa aaaacgggtc
    20281 accttctggc gacccgtttt tctttgcgta attagtggct aaccgtctgt gtacctgtcg
    20341 ggacacgaac gtgtttatat ttgaacatcg ccatgaacgc gaaggccaga accacggagt
    20401 aaccagcgaa aatcaaccat acggtctgcc agtcggtaat gccgttttgg gtgtacatct
    20461 caacaacttt accgctcacg atgccgccga ggatacagcc gaagccgtta gtcatcatca
    20521 ggaacatccc ttgtgcactg gcgcgaattg ccgggctaac ttctttttcg acaaacaccg
    20581 aaccagagat gttgaagaag tcgaatgcgc aaccgtaaac gatcatcgac agtaccagca
    20641 gtacagtacc gaacggagtc gggtcgccgt aagcaaacag cgcaaaacgc aggatccacg
    20701 ccacaatact gatcatcatt acgttcttaa taccgtagcg gcttaagaag aacgggatgg
    20761 tcagaatgaa cagggtttca gagatctgcg aaatcgacat gatgattgac gcatgctgca
    20821 caataaagct gctggcaaac atcggatctt tgtcgaagct gtgcaggaag gtattaccga
    20881 acatgttggt aatctgcagt tccgcgccca gcagcattga gaagatgaag aagattgcca
    20941 tacgcttgtt tttaaacagc gcgaatgcat cgaggcccag cagggttgtc cagctctgat
    21001 tcgcttgctg tttagcaacc ggaatatgcg gcagagtcag ggtaaacaga accagaatgg
    21061 cggaaagtgc tgcgccaata tacagctgca tgtggcttaa ttcgaagcca gacaggctca
    21121 ccacccacat tgccatgata aagccgatgg tgccccagat acggattggc gggaagtcag
    21181 taacgatatc catcccggca ttttgcaggc gatagtaaga gatggtgttg attaacccaa
    21241 gcgttggcat ataagcaaac gagttaatca atatcacaag gaacatcgcc tccggcgtcg
    21301 tgacctgtgc cgccatgaac agcgtgatag cgccaatggt gtggcaaatg gcatataccc
    21361 atttcgcact taaccatttg tcggccacaa tccccagcag cgcaggcata aagaccgcgg
    21421 cgatacccag tgagctataa actgcgccaa tagaagcacc gtcaaacttc agggtaacaa
    21481 acatatagga accgagggtc gtcagccaac ttccccacag acagaactgc agaaaagaga
    21541 ggattttcag ctgcagctta agattcatgt taatttcctc acatcgtgat gcggatgagt
    21601 gtttgaaaaa gcacatttga ccgtgcgaga tgatgggatt ctatcaactt cgcggactgg
    21661 ttttttgtta cctgtggcaa ataattgcaa acattcctaa tttgcaaaac gaaattgtga
    21721 tatggataac atttttgccc ctgagcatcg tcaggggcgg ttagtggaat tatcggcggc
    21781 ggtaggattt ttgcgcggta ttcactttat aaagataacg gcgagattcc gcagagggat
    21841 ggcgggtcgt cagcgtttga taaacatcgc ccggcgtcat ggtgttaata atattagccg
    21901 cctgaatctt atcattcgaa aagactcgca gcacgctgcc tgcgccgccg ttataggcgg
    21961 tgatgacggc ataacgccgg gatgtagggt tatcaattcc gccgagataa acattgttca
    22021 gcatcgccag atacgcggtg ccggtatcaa tattgctggc aggatcaaac aagaaactgc
    22081 ggctcggcgt gccggatttc ccctgcgagc ggaacacatc tttcccggca gtatgttgta
    22141 ccacctgcat taaccccagc gcatcggaac ggctgaccgc atacgggtta aaggaagatt
    22201 cagtctgcat aattgccaga atcagcgact catcaacgcc atatttccgt gacgcctggc
    22261 ggaccatgcc gagatatttg tgcgcacgtt tatcaaggtg gttcggcacc atgttaatgg
    22321 tgacgctgta gatgatacgc agcccgttgc tgcggctctt cagacggttt ttcagcagat
    22381 aatccgcgaa gttgcttgcg cgaccttccc agcgaatcgg ctgcccggtg ttgtccacca
    22441 cctgaccgta aaggaaaggt tctttcgaaa tcgtaatatc atcaacgtcg gaatagagat
    22501 cgaccgaact cggatcgtca cccatcaata acgttttgat aattgcccgg cgcaaatgcg
    22561 cggcaggttc tgtcccggcg atggtttcga tagtaatcgt accgtcatcg aagttgatgt
    22621 ggctgcgggt ctgatattga tcggtgtatt tcacgtagtc cttaggacca gcgatcacca
    22681 cctctttgaa gccccagatg ttctcaatat tgtgggcaaa ttgccccatc agaatatcaa
    22741 aaccgttggt atctttgacc caggcttcgt tataggtatc gccttttttg gtcgtcgaac
    22801 aggagatgag caacggcgca atcaaagcca gcgcgagata ttttttcatc attccgggtg
    22861 cgtgttgtgt ttgtttttat gcaagctgcc ggaggcgtgc tccggcactg tttttatttt
    22921 ttatcttccg gcgtatagcc ctcgatatgc acctctttac cctcgaacag gaagttgacc
    22981 atctcctgct ccagcagctt gcggtgctcg gcattcatca tgttgagttt cttttcatta
    23041 atcagcatgg tttgtttgtg ctgccactgc gcccaggctt ctttggagat ctcgttatag
    23101 atgcgttttc ccagttcgcc ggggtacagc tgaaaatcct gaccttctgc ttcacgttgc
    23161 aggaaagtac aaaaaatcgt tctgctcata aatcatcctc tttatcgact cacgcgctaa
    23221 accggcgcgc cagtgcgtaa ctgctgtaac aaacgctcca cgggagccgc caggccaact
    23281 gacggcggtt gcgctaagtt ataccagagc gcattgcctt catccatgca gccggtgaat
    23341 gacgacacgg gaagccacat aggcacaata tctaagtgga aatggctgaa ggtatgccga
    23401 aacgcggtca gctgcgtcag gttatcggca gcaatctgcc gttgcgccag ccactgccgc
    23461 aaactttctt cgtcggcaaa ctgcgggaaa cagtataaac cgccccacaa tccgctcggc
    23521 ggacgctgcg ccagcaatac ttcatcttcg tgctgtaaca ggagaaagta gccggtgcgc
    23581 tccggcagcg tctgtttcgg ttttttgccc ggataaagcg accagctatt gttggtggcg
    23641 gcaatacatc cgttttgtag cggacagagc gaacatttcg gcttcgaacg cgtgcaaatc
    23701 atcgcgccca aatccatcat cgcctgatta aaccgttcca cgccgaccgc gggcgtcacc
    23761 tgctcgctta aactccataa tttattctcg acctcttttt tcccaggcca gccgcttaca
    23821 gcatagcagc gcgccagcac ccgtttgacg ttaccgtcga gaatcggaaa gtgcttaccc
    23881 agagaaagcg agagaatcgc gcctgcggtg gaacgcccga cgcccggcag tgctgcaact
    23941 tcctcaaagg tttccgggaa tttaccgccg tgtaaggtcg ccacttgttg tgccgcttta
    24001 tgcagattgc gcgcgcgggc gtaatagcca agccccgtcc acaagtggag aacttcgtcg
    24061 agcggcgcat tggcgagatc ggtcaccgtc gggaagcgcg ccataaagcg ttcaaaatag
    24121 gggataacgg tcgcaacctg agtttgttgc aacatcactt ctgagagcca tactttgtag
    24181 ggcgtcttgt caatttgcca gggcagagtt tttcgcccgt atttatcgta ccagtccaga
    24241 acctgggctg aaaattgcga cgcttgcatg gtcaccgaat tcactgttgt tgggggcaag
    24301 attgcagcac agcgacggca gggtgtaaac cggaactttc cgcagccacg gccttatcat
    24361 gcttgcatcc ggcaactaac tttggataat gcccgttttc agaacacttt cacaagcgac
    24421 taaatcttta tgaaaaacga cgtcatttca ccggaatttg atgaaaacgg ccgcccactg
    24481 cgccgtatcc gtagttttgt gcgccgccag gggcgactga ccaaaggcca ggaacatgcg
    24541 ctggaaaact actggccggt gatgggcgtt gagttcagcg aagatatgct ggatttcccc
    24601 gcgctttttg gccgtgaagc gccggtgacg cttgagattg gttttggcat gggggcgtcg
    24661 ctggtggcaa tggctaaaga tcgccccgag caggacttcc tcggcattga agtgcattca
    24721 ccgggcgttg gtgcgtgcct ggcttctgcg catgaagagg gcttaagcaa cctgcgcgtg
    24781 atgtgtcacg atgcggttga agtgctgcat aaaatgattc ctgacaattc attgcgcatg
    24841 gtgcagctct ttttccctga cccgtggcac aaagcgcgcc ataataaacg ccgtatcgtt
    24901 caggtgccgt ttgccgaact ggtaaaaagc aaactgcagc tggggggcgt attccatatg
    24961 gcgaccgact gggaacctta tgcggaacat atgcttgaag tgatgtcttc tattgacggt
    25021 tataaaaacc tgtcagagag caatgattac gtaccgcgtc cggcatcacg tccggtgacg
    25081 aaatttgaac aacgtggtca tcgtcttggt cacggagtat gggacttaat gttcgagagg
    25141 gtgaaataat ggcaaagaac cgtagccgtc gtctgcgtaa aaaaatgcac atcgacgaat
    25201 tccaggaatt aggattttcg gtggcatggc gattcccgga aggtacatcg gaagaacaga
    25261 ttgataaaac cgttgatgat tttattaacg aggttatcga accgaacaaa ctggcctttg
    25321 acggcagcgg ttatctggcc tgggaaggtc tgatctgcat gcaggaaatc ggcaaatgca
    25381 ccgaagaaca tcaggcgatt gtgcgtaagt ggctggaaga gcgcaaactg gatgaggtac
    25441 gcaccagcga acttttcgac gtttggtggg actaagaaag catacgggcg atgacaaatg
    25501 caaaactgcc tgatgcgcta cgcttatcat gcctacaaag atgcacgatc gagtaggcgg
    25561 gataaggcgt ttacgccgca tccggcatgg aaaacgcgta ctttgttatc aatctggggc
    25621 cagcaaatgc tggcctgatt tgttcttgag ggaagactat gatgcgcaaa atgctgctgg
    25681 cggcagcact ttcagtgacg gcaatgaccg ctcacgccga ctaccagtgc agcgtcacgc
    25741 cgcgtgacga tgtgattgtc agcccgcaaa ccgtgcaggt gaagggcgaa aacggcaatc
    25801 tggtgatcac gccagacggc aacgtgatgt ataacggtaa gcaatattcc ctgaatgccg
    25861 cccagcgcga gcaggcgaag gattatcagg ctgaactacg tagcacgctg ccgtggattg
    25921 atgaaggcgc gaaaagccgc gtcgagaaag cccgtattgc tctggataaa attatcgttc
    25981 aggagatggg cgaaagcagc aaaatgcgca gccgtctgac caaacttgat gcgcagctga
    26041 aagagcagat gaaccgcatt atcgaaacgc gcagcgatgg cctgacgttc cactataaag
    26101 ccattgatca ggttcgcgct gaaggccagc aattagtgaa tcaggcaatg ggcggaattt
    26161 tacaggacag cattaatgaa atgggcgcga aagcggtgct gaaaagcggc ggtaacccat
    26221 tacagaatgt gctgggaagc ctgggggggc tgcaatcctc aatccaaacc gagtggaaaa
    26281 agcaggaaaa agatttccag cagtttggca aagatgtttg tagccgcgtt gtgactctgg
    26341 aagatagccg caaagccctg gtcgggaatt taaaataatc ctctatttta agacggcata
    26401 atactttttt atgccgttta attcttcgtt ttgttacctg cctctaactt tgtagatctc
    26461 caaaatatat tcacgttgta aattgtttaa cgtcaaattt cccatacaaa gctaagggat
    26521 aatgcgtagc gttcacgtaa ctggaggaat gaaatggagt ttttcaaaaa gacggcactt
    26581 gccgcactgg ttatgggttt tagtggtgca gcattggcat tacccaatat caccatttta
    26641 gcaaccggcg ggaccattgc cggtggtggt gactccgcaa ccaaatctaa ctacacagcg
    26701 ggtaaagttg gcgtagaaaa tctggttaat gcggtgccgc aactgaagga cattgcgaac
    26761 gttaaaggcg agcaggtagt gaatatcggc tcccaggaca tgaacgatga tgtctggctg
    26821 acactggcga aaaaaattaa caccgactgc gataaaaccg acggcttcgt cattactcac
    26881 ggtaccgaca cgatggaaga aaccgcttac ttcctcgacc tgacggtgaa atgcgacaaa
    26941 ccggtggtga tggtcggcgc aatgcgcccg tccacctcca tgagcgcaga cggtccattc
    27001 aacctgtata acgcggtagt gactgcagct gataaagcct ccgctaatcg tggcgtgctg
    27061 gtagtgatga acgacaccgt gctggatggt cgtgacgtca ccaaaaccaa caccaccgac
    27121 gtagcaacct ttaagtctgt taactacggt ccgctgggat acattcacaa cggtaagatt
    27181 gactaccaac gtaccccggc acgtaagcac accagcgaca cgccgttcga tgtctctaag
    27241 ctgaatgaac tgccgaaagt cggcattgtt tataactacg ctaacgcatc cgatcttccg
    27301 gctaaagcac tggtagatgc gggctatgat ggcatcgtca gcgctggtgt gggtaacggc
    27361 aacctgtata aaaccgtatt tgacaccctt gcaaccgccg cgaaaaacgg cactgcagtc
    27421 gtgcgttctt cccgcgtacc gacgggcgct accactcagg atgccgaagt ggatgatgcg
    27481 aaatacggct tcgtcgcctc tggcacgctg aacccgcaaa aagcgcgcgt tctgctgcaa
    27541 ctggctctga cgcaaaccaa agatccgcag cagatccagc agatcttcaa tcagtactaa
    27601 tcgcctcgcc ccggtatcgt gccggggctt tttcacttca gacttacgtc cattgccaat
    27661 tttcattacc ctaatgataa tcaccggaat aaattattcc gcgcgagggt tttcgggtga
    27721 aaaagcaatg gattgttggc acggcgctgc ttatgttgat gactggtaat gtctgggcag
    27781 atggcgaacc gccaactgaa aatatcttaa aagatcaatt caaaaagcag tatcacggca
    27841 ttctcaaact tgatgccatc accttaaaaa atcttgatgc taagggtaat caggccacct
    27901 ggtcagcgga aggcgatgtc tcttccagtg acgatctcta tacctgggtc ggtcagttgg
    27961 cagattacga actgctggaa cagacctgga cgaaagataa accggtaaaa ttctcggcga
    28021 tgttaaccag taaaggaacg cccgcgtctg gctggtcggt gaacttttac tcttttcagg
    28081 cggcagccag cgatcgtggg cgggtggtcg acgatatcaa aacgaataat aaatatctga
    28141 tcgtgaatag cgaagatttc aattatcgct ttagtcagct tgagtctgcg ttgaataacc
    28201 agaagaactc gattcctgcg ctggaaaagg aagtgaaagc gcttgataag caaatggtgg
    28261 cggcgcagaa agcagcggat gcatattggg gaaaagatgc aaacggtaag caaatgaccc
    28321 gcgaagacgc ctttaaaaaa attcaccaac agcgtgatga gtttaataaa cagaacgata
    28381 gcgaggcgtt cgcggttaaa tatgacaaag aggtttatca accggcgatt gcggcatgtc
    28441 ataaacagag tgaagagtgt tatgaagtgc cgattcagca gaaacgagat ttcgatatca
    28501 acgaacaacg gcgacagact tttctgcaat cacaaaaact tagccgtaaa ttgcaggatg
    28561 actggataac gctcgaaaaa gggcaatatc cgttaacgat gaaagtttca gaaataaaca
    28621 gtaaaaaagt cgcgattttg atgaagattg acgatatcaa taaggcctgc gatcgctgga
    28681 aaaaagatac cgaacaactg cggcgcaatg gggtgattaa gtaagttttg atatagcgca
    28741 cgatttgtag caggatgcgg cgttgacccc gcatccggca atcaacgtct gatgcgacgc
    28801 tagcgcgtct tatcagacct acaaccgttg ccacatgtag gctggataag gcgttcacgc
    28861 cgcatccggc aatacaagtt tactcagcca gaaaaagctc cagcagcgaa tttaaaaaca
    28921 acttcccatg ttccgttatc tgccagtaat ccgcacattc tgtgagataa ccctgggcaa
    28981 tcgcctcgtc taactgtggg cgaatcacat cttcgcaaag cccggtatac gcactaaact
    29041 ctgcgcgtgg cgcggcctcc agcaagcgaa agcggttcat aaagaactca aacggcttat
    29101 ctgcagcttc gacatcacgc tggctttcca gataccttcc ttgcataaaa ccacgcggat
    29161 gacgcgtttt ggtggtacgc agaatgcgcc catccgggaa ggtcactttg ccgtgcgcgc
    29221 cgcagccaat accgatgtag tccccaaagc gccagtagtt gagattgtgc tggcactggt
    29281 aaccgggttt ggcgtaagcg gaagtttcat attgctgata acccgctgcg gttaataact
    29341 gatgcccctg ttcgaatata tcccacaacg cgtcatcgtc cggcagtaca ggagggcgcg
    29401 agccaaacag cgtattgggt tcgatggtca gttgatacca ggaaagatgc ggcggattca
    29461 gttcaatggc ctggcgcaga tcgccaagcg cctcttccag tgattgatcc ggcagcccat
    29521 gcatcaaatc aaggttaaaa ctacgcagcc ccagcccgct tgccagattc gcagctcgtt
    29581 tcgcttcttg cgggccatga atgcgcccaa gtcgtttcag cttttcttcg ctaaaactct
    29641 gtacgccaat agagatgcgg ttcacgccag cacgctgata atcgacaaag cgatcggctt
    29701 ctaccgtacc agggttcgct tccatagtaa tttctgcatc cgctgccagc ggcaaacgcg
    29761 cacgcacgcc gtccagcagc gtttgcatcg ccgggccgga aagcaggctc ggcgtaccac
    29821 cgccaataaa aattgtctgc acttcacggc cctgagcgta agccacatcg ttgtccagat
    29881 cgttaagcag atgctgaaca taatcgtcgt gcggcacttc tcctttcaac gcgtgagagt
    29941 tgaaatcgca gtacgggcat ttctgcacgc accacgggat gtgaatgtag agactcagcg
    30001 gcggtaattt aaccattacg taaagcgtcc agcagcagtt tcaacgcctg accacggtgg
    30061 gaaatggcgc tcttttgttc gcgagtcagt tcggcagcgg ttttcccttc ggaaggtacg
    30121 aagaagattg gatcataacc aaagccacca gtgcccgccg gttcacgagt aatcactcct
    30181 ggccagctac cgtggcacac cagcggagtc ggatcttccg cgtgacgcag atacaccagc
    30241 acgcagtgga aacgcgcctg acgttggtcg tccggtacgt ctttcagtgt ttccagcagt
    30301 ttttgcagat tcttttgatc ggtcgcgtct tcaccggaat aacgcgcgga gtaaatgcct
    30361 ggcgcgccac caagtacatc taccgccaga ccggagtcgt cggcaattgc aggtaaaccg
    30421 gtcacttttg ccgcatggcg cgctttcaga atcgcgtttt cgataaaggt caggccggtt
    30481 tcttcagcgg aatctacgcc gaggtctgtt tgggccacga tatcaagacc gaagtcgcta
    30541 agcagcgacg ccagctcacg cactttaccg gcattgccgg ttgcgaggac aactttttgc
    30601 ataggatacc taattaatta acgccgcgat ttctggcggg atttgttgcg gattaatgat
    30661 tttaatttgt ttgtggcggc caagttcgcc tttttcaatc accacctggc ttttggcaac
    30721 ccggaattgc ttaccgagaa acttcaccag atgactgttg gcctggccgt caaccggcgg
    30781 cgcggttatg gcgactttta cctcgtcgcc atgtaaaccg acaatagaat cacggctggc
    30841 tttcggctga atataaagcc gtaaaaccag accgtcatcg ttaactgtta cggcactcat
    30901 aacgccatcc acagccccgg cagcagcatg tttccggttg cctgtaatac ttctgcgaca
    30961 cccatattga tgacatacag cagcagaacg aggatcatcg gcgagaaatc aatcccaccc
    31021 attgccggta gcaggcggcg aatcgggcgc agcagcggat cggccagctg aatcagcacg
    31081 tattcaatcg ggctacggcc ctggcttacc cagctcataa tcgccatcac cagcaggacc
    31141 cagaaaatca gcaggccgat ggttttcagc agaatcagta aaccggcaat ccagatgatt
    31201 ggcaggaagg tcaccacttt aaacagcacg atggctttga taaaactgag aatataggca
    31261 accagtagcg aggcgctatc aatggggccc attgccggaa taacgcggcg cagtggcccg
    31321 ataattggct gcgttacctt cactacaaac tgtgagaagg ggttgtaaaa atcacaatga
    31381 gcccactgca tccagatgcg taataacagc accatggtat acagctcaat gaccgttgaa
    31441 agcaggaaag tcaacgtatt catggcgttc ctttaattcc ttatttttta gagtaatcac
    31501 gcgcaccaaa aattgcagtg ccgatacgaa ccatcgtgct acctgccgca atggcggctt
    31561 ccatatcgtc cgacattccc agagagagcg tgtcgatatg cgggtagcgc gttttcagtc
    31621 cggcaaatgc tacagccatt tggcgtgcaa cttcaaactg ccttacatat tctgactcag
    31681 gcgcagggat tgccatcagc ccgcgcagac gtaaacgcgg tagttcagcg accgctgctg
    31741 ccagctcgtc cagttctgcc agttgaatcc cggacttact gttttcatca ctaatattaa
    31801 tttgaatcag cacgttaaga gggggaagtt ctgccgggcg ctgatcgttg aggcgggtag
    31861 cgatgcgcaa acggtcgatg gtatgacacc agtcgaaatg ctctgccacc aggcggcttt
    31921 tattagattg caacgggcca ataaaatgcc attctaatcc tgttacgccc agttcctgaa
    31981 agtggcgaat tttatctacc ccttcctgaa cgtagttttc accaaattga cgctgcccgg
    32041 catcaatggc ttctgcgatg gcgctcgcag gttttgtttt actgactgca agcagcgtaa
    32101 tttcttctgg agaacggccg caacgcgttg cagccgctga gattttgtcc cggacctgtg
    32161 ccaggttatg cgcaatatcg ttcattttcc gaggatctta gtatgaatat ggaagaaatt
    32221 gtggccctta gtgtaaagca taacgtctcg gatctacacc tgtgcagcgc ctggcccgca
    32281 cgatggcgca ttcgcgggct aatggaagct gcgccgtttg atgcgccgga cgtcgaagag
    32341 ctactgcggg agtggctgga tgacgatcag cgggcaatat tgctggagaa tggtcagctg
    32401 gattttgccg tgtcgctgac ggaaaaccag cgattgcgtg gcagtgcgtt cgcgcaacgg
    32461 caaggtattt ctctggcgtt acggctgtta ccttcgcact acccgcagct cgaacagctt
    32521 ggcgcaccac cggtattgcc ggaattactc aagagcgaga atggcctgat tctggtgacg
    32581 ggggcgacgg ggagcggcaa atctaccacg ctggcggcga tggttggcta tctcaatcaa
    32641 catgccgatg cgcatattct gacgctggaa gatcctgttg aatatctcta tgccagccag
    32701 cgatgtttga tccagcagcg ggaaattggt ttgcactgta tgacgttcgc atcgggattg
    32761 cgggccgcat tgcgggaaga tcctgatgtg attttgctcg gagagctgcg tgacagcgag
    32821 acaatccgtc tggcgctgac ggcggcagaa accgggcatc tggtgctggc aaccttacat
    32881 acacgtggtg ccgcgcaggc agttgagcga cttgtggatt catttccggc gcaggaaaaa
    32941 gaccccgtgc gtaatcaact ggcaggtagt ttacgggcag tgctgtcaca aaagctggaa
    33001 gtggataaac aggaaggacg cgtggcgcta tttgaattgc tgattaacac acccgcggtg
    33061 gggaatttga ttcgcgaagg gaaaacccac cagttaccgc atgttattca aaccgggcag
    33121 caggtgggga tgataacgtt tcagcagagt tatcagcagc gggtggggga agggcgtttg
    33181 tgacgttgcc atgcccgatg tgcaagctta tcgggcctac aaaggcaata aacgtctgat
    33241 gcgacgcttg cgcgtcttat cagacctaca accgctgcca catgtaggct ggataaggcg
    33301 ttcatgtcgc atccggcaat aagacgcgat gatattattt acactccgcc aggcttttaa
    33361 atcgccttaa tatccctgct cgaaatagct ttcgagaata ataaccgcag aggcagagtc
    33421 gactttgcct ttgttaagcg cccgatagcc gccctgttca aacagaccgg aacgggcttc
    33481 cacggtgcta agacgctcgt catggagctt tacttcaaca ccgaaacgac catgaatacg
    33541 gttggcaaat ttacgcgctc tggcggttaa cggctgttcg gtaccgtcca tattcagcgg
    33601 caaaccaacg atgatttcgt ccggctgcca ctctttcagt aaacgttcga tgagattcca
    33661 gtccggcgta ccgtcctgtg ctttaattgc aggcaatggg cgagcggtgc cggtaatgcg
    33721 ttggccgacc gctacgccaa tgcttttggt accgaagtcg aaggcgagta aggttccact
    33781 catcaggcgt gtcctgccac accaggcatg gtgagaatat ccacaccaat cagttttgcc
    33841 gcctcgcgcc agcggtcggc aattggcgtt ttgaacagaa tattcagatc tgccggggcg
    33901 gttagccacg cgttatcgag aatttcttgt tccagttgac ctttctccca ggaggcataa
    33961 cccagagcca ccaatacgtc agacggttgt ttatcggtgc cgagcgtttc cagcacatcg
    34021 cgggaagtgg tcattaccgt gttgtctgaa atgcgaatgc tggaagcaaa attagagggc
    34081 ggagtatgca aaataaaccc gcgatcttca gccagtgggc cgccgagcat aaccggttta
    34141 tccagacgga ttgattcatc acgcggctcc ggcgtgatct tcagcttttc cagaatccct
    34201 tcaattttga gattttccag cggcttgttg acgatgatcc ccattgcacc attggtatta
    34261 tgttcgcaaa tgtagaccac ggaacgacgg aaaatcggat cctggagagc aggcatggca
    34321 ataagaaagt gatgctgtaa attcattgtc agaggttctg tttcctggtt caaaaaagca
    34381 acagcgccca gtatgcgtgg aaagaagggc gctgtcactc agagtctcaa cgagatcctt
    34441 ctcgctaagg tgggttactg ctgctgtaaa cgtgcttcaa tggcatccat taacattcca
    34501 gtgatcgaca ccggaaactc tgcttcaatc tcacgaatac aggttgggct ggtgacgtta
    34561 atttcagtca gacggtcgcc gatgatatcc agaccaacaa aaatcagccc tttttctttc
    34621 agcgtcggcc cgatctgacg ggcgattttc cagtcacttt ccgtcagcgg acgaggttca
    34681 ccgcgaccac cggcagccag attgccacgg gtttcgcccc cctgcggaat acgcgccagg
    34741 cagtacggta ccggctcgcc atccaccacc agcacgcgtt tgtcgccatc tttaatggct
    34801 ggcaggtaat tttgcgccat gcagtagcga gtgccatgct cagtcagggt ttcggcaatc
    34861 acgccgaggt ttggatcgcc ttctttcacg cggaaaatcg acgcgccgcc cataccgtcc
    34921 agcggcttaa gaatgatgtc gctgtgtttc tcccagaacg cttttagctg cgctttattg
    34981 cgcgtaacca gcgtttctgg cgttaagtca gagaaccagg cggtaaacag tttctcgtta
    35041 cagtcgcgca ggctctgcgg cttgttaacg atcagcgtcc ctttctcttc ggcacgttcc
    35101 agaatatagg tcgcgtagat aaactcggta tcaaacggcg ggtctttacg catcaggatc
    35161 acatcgagat cggccagcgg cagatcctgt tcaccgacga atgaaaacca ctcttcgtag
    35221 ttctgcttca cgttcagcgt gcgggtatgg gcgcgggctt cgccattgat cagatacaga
    35281 tcgcccatct ccatatagtg aagttcgtaa ccacgacgct gtgcttccag caacatagcg
    35341 aaactggaat ctttcttgat gttgatgttt gcgatggggt ccatcacgat gccgagcttg
    35401 atcattattc ttctccgtta gcccaaatcg ccaaatcgta cttgtagcgc ggtaatggcg
    35461 gtgagcgcag ttgtctctgt acgcaaaacg cgaggtccca acaggatatc agtaaattga
    35521 tagcgggcag tcatggcaat ttcatctgcc gataaaccgc cttccgggcc aatcagcagg
    35581 cggacgcgtt caaccggtaa aggcaacgta ttgatgctgt tactggcgcg cgggtgaaga
    35641 ttcagtttca gtccttcatc ctgctctgca caccaggctt ccagatccat cgctggacgg
    35701 atttccggca ctcggttacg accacactgc tcacaggcag caattgcaat cttctgccac
    35761 tgctggagct tcttgttcag acgttcacta tccagtttaa cgccgcagcg ctcagaaaaa
    35821 agtggcgtaa tgaggcttac accgagttcg atcgatttct ggatcgtaaa ttccattttt
    35881 tcaccacgcg acatcacctg accgaggtga atatgcagcg gagattcgcg atcgtcgatc
    35941 tggccttcca gcaccttcac ttccacgctt tttttgctgg cgctggtaat ttcggcgtca
    36001 aagacctggt tgctgccgtc aaacaattgc aacgcctgcc ccggccccat gcgcagtacg
    36061 cgcccgatat ggttggcggc atcttcgcaa agcgcgatgt gagaatggct ggtcagtggt
    36121 tcaggatgat aaatgcgggg gatacgcata gttaaaaatc cgcgtcgtct ccccacgcgg
    36181 ttgtacgcgt ggggtagggg ttaacaaaaa gaatcccgct agtgtaggtt agctctttcg
    36241 cgcctggcaa gcgcgttgca catacgggtt atgattgccc tgcaccttcg cgatgcgttc
    36301 atcgcgctcg cactcccagt cggtaaccgg atacatcttg ttccatgcgt tgaacagctg
    36361 cgtttgctgg cgagagagtg tcaggttgta ttggtcacgc atatagaagt aggtgcgcgc
    36421 aatggcaccg cgtgcacgcg ctggtggttc ggcagctttt tctttgaaat cgaccttcat
    36481 ggcgcattga ccgtactggc cttcgccgcc attccactgg ctgtacataa agttgccgcg
    36541 atcgccattc acctcgccga ctgacggctg caggttatgc atatcgcttt ccatcttgcg
    36601 atagaccgga tctttagcgc agtttttacg tccaccgtcc tgccagcact ggcgctggtg
    36661 accgaattgc caggcgggaa cgacgtgttc ccactctacg cggctggcgc ggttttcatt
    36721 tttgcgcacc tgatagccgc acgattgcag atcaacaacg ccttttttgc cctgccagtt
    36781 aattttacat ccgcaataaa acgtaccggg cgcgtcagcg tggactttta ccgccgcggc
    36841 tttcgcctga gaaaaactat tgataccttc ggccaacgcc gggccggaaa atgctgcgct
    36901 cagtaccacc gcagcaatag acaaataacg gtacatcact tactccgtgt taaaacgagc
    36961 cagcaacgta gcgaaagttg ttttggtatg caatcagcca gatcaggaaa gttcctgata
    37021 gttcagatgg ttattttgcg accagttgtt cgccgcagtg aacacagcga tagacggctt
    37081 cgccacgaac tacgcgatta tggcggcgta cggtaagctg atgctcctgg cacttgcagc
    37141 ggtaggggaa ggtgttgcga cgcacggatt gcagttcgaa ctgatgcgta cgacgggctg
    37201 gaacgcccag cacactttcc atcatccact tccactcttt gccatgtggt gcaacgcggc
    37261 caaaatgttt ccataccagc aaatgtgcca gttcgtgcgg caccacttct tcaataaagg
    37321 cttcactgtt ttccaacagc aaaacgggat tgaggcggat ttcatagctt tccagccagg
    37381 ctgttccggc ggaggttccg cgctgggtgt aagagagttt tggctccggg tagttacgcc
    37441 ctagcttcag gttggcctgg gcgagttttt cccgcaggcg acgcataacg gcctgttgga
    37501 tggcgatagg gagacgggat gttttcatag cggcagagga tagagcgaag aaaagatgac
    37561 tgcaagaggc ggcttcctcc gcgacgggag gaagcctgag gagattaatc gtgagcgccg
    37621 atttcgcgca gtttacgacc tttcatcaga ttacgttcaa tatgttccag cgaaacgtgt
    37681 ttggtttccg gtaccagcca caatgtcagc aggataaaca gtacgttcag agccgcatac
    37741 acccagaagg tgttggcgtt acccagcgtg ttgagcatgg tcaggaacgt tgcgccaacg
    37801 atcatgttgg caatccagtt ggtggcggtg gagcaggtga tgccaaaatc gcggcctttc
    37861 agcggctgaa tttcggagca cagtacccaa atcagcggac cggcactcat ggcaaaaccg
    37921 acaataaaca tcagcagcat ggcgatggcg aaatactgcg ccgacggaga gtggataccg
    37981 atatgcatca ttgtaccgag tacgcccatg ccagcagcca tcaccaggaa gcccagcgtt
    38041 agcgttggtt tacgtcccca gcggtcaaca agaccgattg cgataaaggt ggcaagtacg
    38101 ttggtcaggc cgacaatcac tgtcccccac atttgctcgg tagtgttggt ataacccgcc
    38161 agttcgaaga ttttcggcgc gtaatacatg atgacgttca tcccggtgaa ttgctgcatt
    38221 acctgtaaca gtacgccaag gaacaccgcg cggcggaagt tgctattctc tttaaacagc
    38281 gcccagccac tctgtttaac ctgcaaactt tcacggattt catccagttc gcgtttcgct
    38341 tccgcgctgg tgtcacgcag gcgtagcagc acgcgttcgg catcaacaaa acggcgtttg
    38401 gcggcaaacc aacgtgggct gtctggcagg aagaagacac caatcagcag caaaattgcc
    38461 gggatgataa tcacacccag catccagcgc catgcaccgg tgtagctgaa ggcagtatca
    38521 gaaagataag caccgaggat cccgatagtg atcatcaact gatacatcga gatcatactg
    38581 ccgcgaattt tttccggcgc aatttcagag aggtacagtg gtgcggtata agaggccaca
    38641 cctaccgcca gccccagcag aacgcgggaa agaatcagta cttcaacgtt tggcgcagcc
    38701 gcagagaaca gcgaaccggc aacaaacaaa attgcgccga tcatcaggct ctttttgcgc
    38761 ccgagtttaa aggagagcca gccgctgccc accgcaccga ctgccgcacc gaacatcatg
    38821 gagcttacga cccattcttg cgtgtgcgaa gtaatctgga attcatctgc aataaacggc
    38881 agtgcgccag caattacacc gatatccagg ccaaagagta atcccgccag agcggcaagg
    38941 aagcagacga aaaacgtcat tgccttgttt gaccgcccct gttttttagc gtcaggcatg
    39001 atgccctcca atatggttat tttttattgt gaattaagat aggtgagtac gacgtaaaaa
    39061 gatgtgaagc aaatcacatc agtgtaatcg cttacactaa catttatctt agttatgatt
    39121 taagtgcatg aaaataattg agttatatca tcatagatta aacgctgtta tctgcaatta
    39181 agactttact gaaaagaaat gtaacaactg tgaaaaccga ataataatat gaaaatatta
    39241 caaatctttg tctgttgtgg ttggtgtaat cggtttcagt tgtgatgata atttgcggtg
    39301 aaacgtgatc aggaagaatg atgttattgt gcaggcggtt tttttgccgg atgcggcgtg
    39361 aacgcctatc caccgcggtg aggtaaaaga aaaaggccag cgcgaggctg gcctttgaaa
    39421 gcaggtgaag aaagattact tcagaccggc agcatcgcgc agcagctgcg ctttgtcggt
    39481 tttttcccac gggaaatgtt cacgaccaaa gtgaccgtat gctgcggttt ctttgtagat
    39541 cgggtgcagc agatccagca tctgaatcag accgtatggg cgcaggtcga agaactcacg
    39601 taccagcaga gtcagttgtt cagaaggcac tttctcggta ccgaaagttt ctaccatgat
    39661 ggaagtcggt tcagccacgc cgattgcgta ggaaacctga atttcacaac gatcggctag
    39721 gccagcagca acgatgtttt ttgcaacata acgtgctgcg tacgcagcgg aacggtccac
    39781 ttttgatgga tctttaccag agaatgctcc gccaccgtga cgtgccatgc cgccgtaggt
    39841 atcaacgata attttacgac cagtcagacc gcagtcaccc atcgggccac cgataacaaa
    39901 acgaccggtc gggttgatga agaatttggt ggcagaagtc agccattcag cgggcagaat
    39961 cggcttgatg atctcttcca ttaccgcttc ttgcagcgat ttctggtcga tctcttcaga
    40021 gtgctgagtg gaaagcacga cagcatcgat accaacgatt ttgccgtcgt catactggaa
    40081 agtcacctgg cttttcgcgt ccgggcgcag ccacggcaga gtgccgtttt tacgcacttc
    40141 agcctgacgc tgtaccagac ggtgtgcata ggtgataggt gctggcatca gcacgtcggt
    40201 ttcattagtt gcgtagccaa acatcagacc ctggtcaccc gcgccctgtt ccagcggatc
    40261 ggcacggtca acgccctggt tgatgtcagg agactgtttg ccgatagcgc tcagaaccgc
    40321 acaggagtta gcgtcaaagc ccatgtcgga atgtacatag ccaatttcgc gaacggtgtt
    40381 acgggtgatc tcttcgatgt ctacccaggc gctggtggtg atttcgccgc caactaaaac
    40441 catgccggtt tttacgtagg tttcgcaagc aacgcgtgct ttcggatcct gttcgaggat
    40501 cgcgtctaaa acggcatcag agatttggtc agcaattttg tcaggatgcc cttcagagac
    40561 ggactcggac gtaaaaaggt gttttgccat atttaatatc acctaaagag aatttggtta
    40621 gctcaaactg ttgtgtggat tttctgtggt agcggatcct accacgactc tgcaggttaa
    40681 aaacactggc agtctgagtg ttaatcggta tggatggatt aacatctgga tggctatttt
    40741 aggtcaattc ttcacccgat ttccactttt ttttgaatcg tgtctcattc tgttaaaaac
    40801 gtggctggaa atttttcctg acaatgccgg cattctgcgt atttatcttt tgcaattttc
    40861 tgccattgtg gggtataaaa cgcggcgcgc ggcttaaata aaaagcacac gacgtttctt
    40921 tcgtgttgcc acttccagcc gggttgaaat cagagttttg gcttgtgggt tcgtcttaac
    40981 aggcggccgt ggaggtgata cgaaataatg aaccgttgtc tgctgcttaa cctgtctcac
    41041 cgttctggtg aagattcgtt ccccgcactc tgcatctctg ctttgcatac ctgccgatgt
    41101 tatacccatc tcggcgcttc tcaggattca agagctggtt acagttactg aggactgaac
    41161 aagggcgctc ttgtaaaaac aagagttttc tcgtggtttc gccgaactgt cacacttacg
    41221 ttcggttatg tgcttaataa tgttatgaaa aagaaaccgg ttgcgcagtt ggagcgtcag
    41281 cattcactgc tggaaaatcc atgtgcttat gggttgttat cgcagttcca ggctgcgata
    41341 gtcgttaact gttttacact taataaaata atttgaggtt cgctatgtct gacgacatgt
    41401 ctatgggttt gccttcgtca gcgggcgaac acggtgtact acgctccatg caggaggttg
    41461 caatgagctc ccaggaagcc agcaagatgc tgcgtactta caatattgcc tggtggggca
    41521 ataactacta tgacgttaac gagctgggcc acatcagcgt gtgcccggac ccggacgtcc
    41581 cggaagctcg cgtcgatctc gcgcagttag tgaaaactcg tgaagcacag ggccagcgtc
    41641 tgcctgcact gttctgtttc ccacagatcc tgcagcaccg tttgcgttcc attaacgccg
    41701 cgttcaaacg tgcgcgggaa tcctacggct ataacggcga ttacttcctt gtttatccga
    41761 tcaaagttaa ccagcaccgt cgcgtgattg agtccctgat tcattcgggc gaaccgctgg
    41821 gtctggaagc cggttccaaa gccgagttga tggcagtgct ggcacatgct ggcatgaccc
    41881 gtagcgtcat cgtctgcaac ggttataaag accgcgaata tatccgcctg gcattaattg
    41941 gcgagaagat ggggcacaag gtctatctgg tcattgagaa gatgtcagaa atcgccattg
    42001 tgctggatga agcagaacgt ctgaatgtcg ttcctcgtct gggcgtgcgt gcacgtctgg
    42061 cttcgcaggg ctcgggtaaa tggcagtctt ccggcgggga aaaatcgaag ttcggcctgg
    42121 ctgcgactca ggtactgcaa ctggttgaaa ccctgcgtga agccgggcgt ctcgacagcc
    42181 tgcaactact gcacttccac ctcggttcgc agatggcgaa tattcgcgat atcgccacgg
    42241 gcgttcgtga atccgcgcgt ttctatgtgg aactgcacaa gctgggcgtc aatattcagt
    42301 gcttcgacgt cggcggcggt ctgggcgtgg actatgaagg cacccgttcg cagtctgact
    42361 gctccgtaaa ctatggcctc aatgaatacg ccaacaacat catctgggcg attggtgatg
    42421 cgtgtgaaga aaacggtctg ccgcatccga cggtaatcac tgaatcgggt cgtgcagtga
    42481 ctgcgcatca caccgtgctg gtgtctaata tcatcggcgt ggaacgtaac gaatacacgg
    42541 tgccgaccgc gcctgcagaa gatgcgccgc gcgcgctgca aagcatgtgg gaaacctggc
    42601 aggagatgca cgaaccggga actcgccgtt ctctgcgtga atggttacac gacagtcaga
    42661 tggatctgca cgacattcat atcggctact cttccggcac ctttagcctg caagaacgtg
    42721 catgggctga gcagctttat ttgagcatgt gccatgaagt gcaaaagcag ctggatccgc
    42781 aaaaccgtgc tcaccgtccg attatcgacg agctgcagga acgtatggcg gacaaaatgt
    42841 acgtcaactt ctcgctgttc cagtcgatgc cggatgcgtg ggggatcgac cagttgttcc
    42901 cggttctgcc gctggaaggg ctggatcaag tgccggaacg ccgcgctgtg ctgctggata
    42961 ttacctgtga ctctgacggt gctatcgacc actatatcga tggtgatggt attgccacga
    43021 caatgcctat gccggagtac gatccagaga atccgccgat gctcggtttc tttatggtcg
    43081 gcgcatatca ggagatcctc ggcaacatgc acaacctgtt cggtgatacc gaagcggttg
    43141 acgtgttcgt cttccctgac ggtagtgtag aagtggaact gtctgacgaa ggcgataccg
    43201 tggcggacat gctgcaatat gtacagctcg atccgaaaac gctgctaact cagttccgtg
    43261 atcaagtgaa gaaaaccgat cttgatgctg aactgcaaca acagttcctt gaagagttcg
    43321 aggcaggttt gtacggttat acctatcttg aagatgaata attaagatta ttccgctggc
    43381 gggtcgattt atttacgtaa atcggcccgc tttcttttta tatattctta aaaataattt
    43441 agctgctttt cttattaaat aatctcccta caatcgctgc attaattaaa tgcagaggga
    43501 acggttatga aaaaatggaa agtacgtagc gcgttgatag cattagtcgt gctgctggtc
    43561 ggttgttcca gtaatgcgca gtataatagc agtgcatcgg gcaatgtcgg caccgcctgg
    43621 ggtggggatg tgcactcatc ggttcagggc gtatctgctg aacgagcctg gcgcgatcct
    43681 gcagagatga tcgttattag ttattcgact aatgttcctt ccggctacga ccgcgtttat
    43741 agcattcgta ttaacgaact ggaatatgct attcgcgatg gtaattttaa ttctctacct
    43801 attacgcggg cttatgattc atccaataat gaaccacgct atattgtcca cgccagagtc
    43861 ggaatgaatt atcaattata tgttcgtaat tacagtcgta acaccaacta cgagatcgtt
    43921 gctacggttg atggcctgga tgtgctgaac ggcaagcaag gttcgctgaa taacaacggc
    43981 tatattgtga atgccggaga ttcgttagcg attaaaggat tccgcaaaga taaacatacc
    44041 gaagcggctt ttcagttcgc caacgttgtt gattcctacg cagccaattc ggcgcagggc
    44101 gatgtgcgta ataccggggt aatcggcttt gccgcctttg aattacaagg cccggcgcaa
    44161 aacgcgctgc caccgtgctc tggacaagct tttccagcag ataacaatgg ctatgcgccg
    44221 ccaccgtgtc ggaaatagca acctcgcttg atgcctcgcc catttaaggc gataattagc
    44281 acaaacttgt gtttcgcaaa tccaaccctt cctcgtcggg cctaacgacg cggaagggtt
    44341 tttttatatc gactttgtaa taggagtcca tccatgagca ccttaggtca tcaatacgat
    44401 aactcactgg tttccaacgc ctttggtttt ttacgcctgc cgatgaactt ccagccgtat
    44461 gacagcgatg ccgactgggt gatcaccggc gtgccgttcg acatggccac ctctggccgt
    44521 gccggtggtc gtcacggccc ggcggcgatt cgccaggttt cgactaacct ggcctgggaa
    44581 cacaaccgct tcccgtggaa tttcgacatg cgcgagcgtc tgaacgtggt ggactgcggc
    44641 gatctggtat atgccttcgg cgatgcccgt gagatgagcg aaaaactgca ggcacacgcc
    44701 gagaagctgc tggctgccgg taagcgtatg ctctccttcg gtggtgacca ctttgttacg
    44761 ctgccgctgc tgcgtgctca tgcgaagcat ttcggcaaaa tggcgctggt acactttgac
    44821 gcccacaccg atacctacgc gaacggttgt gaatttgacc acggcaccat gttctatacc
    44881 gcgccgaaag aaggtctgat cgacccgaat cattccgtgc agattggtat tcgtaccgaa
    44941 tttgataaag acaacggctt taccgtgctg gacgcctgcc aggtgaacga tcgcagcgtg
    45001 gatgacgtta tcgcccaggt gaaacaaatt gtgggtgata tgccggttta cctgaccttt
    45061 gatatcgact gcctggatcc tgcatttgcg ccgggcaccg gtaccccggt aattggcggc
    45121 ctgacctccg atcgcgctat taaactggta cgcggcctga aagatctcaa catcgttggg
    45181 atggacgtag tggaagtggc tccggcatac gatcagtcgg aaatcactgc tctggcagcg
    45241 gcaacgctgg cgctggaaat gctgtatatt caggcggcga aaaagggcga gtaagcacca
    45301 gatgcgatgc gcacgggtaa aacgtgccat tcatgtcaga tgcggggtga acgccttatc
    45361 tgacctacgt ttggcacccg taggccggat aagatgcgcc agcatcgcat ccggcaatgc
    45421 gcacgaggta gtgaatgtgc catttatgtc agatgcggcg taaatgcctt atctgaacta
    45481 cgtttggcac caccatgcct tacttaatcc cgtccgcgct catacgatcg cgaatatgct
    45541 gggcgcgttc ggcggatgct ggatggtcgt caaacattga gctttggcga ccttcttcca
    45601 gttttgccag tttttcaaag ctggtggcaa gacctgccgg actgatgccg cgttggcgca
    45661 gtaaatcgta ggagtaatca tccgcctctg cttcctggcg ctgggagaat tgcgaattga
    45721 ctaatttctc gcccagatca ccaagctgtg attgcgataa actgccgacg ataccgcccg
    45781 cagaggccgc cgcgactcgc acggcatttg taccaagtgc cacctgcatc cctttcttca
    45841 catggcctaa cgccacgtgc cccatttcgt gaccgatcac cgcttcgact tcgttatccg
    45901 tcatcatatc catcagcccg ctatagacgc ggatacagcc gttagccatc gcaaaggcgt
    45961 tcacatcctt cgccatatac actttgtaat tcactggctg accgttgata ttgttgccta
    46021 gcgcgttggc aatggttgtc agacgtttag cgtattcgct attggctggc gcaatcgtcg
    46081 ccttgctgtc catctcctga catgcctgat cgctcagggt tttcacctga gcatcactca
    46141 aactgtaagc ctgaaaagct tccgctcctg atgagagcag tccgttggag tccatattct
    46201 ggcaaccagt cagtaccgtt gccacgctca ttgctaccaa taaggcgcga attttcatgt
    46261 tttttcttcc gcacaaggtc aggattattc taaaaatgcg ccgtttgcag gtgaaccgac
    46321 gctcagtctc agtataagga aaagcgcagc ggacgggcga gtagattgcg caacatgcga
    46381 gcatgatcca gagatttctg aagcggcaaa aggatgttcc atgtacatga cgcgcggctt
    46441 gcggtaaatt gttggcaaat tttccggcgt agcccaaaac gcgctgtcgt caagtcgtta
    46501 agggcgtgcc cttcatcatc cgatctggag tcaaaatgtc ctcacgtaaa gagcttgcca
    46561 atgctattcg tgcgctgagc atggacgcag tacagaaagc caaatccggt cacccgggtg
    46621 cccctatggg tatggctgac attgccgaag tcctgtggcg tgatttcctg aaacacaacc
    46681 cgcagaatcc gtcctgggct gaccgtgacc gcttcgtgct gtccaacggc cacggctcca
    46741 tgctgatcta cagcctgctg cacctcaccg gttacgatct gccgatggaa gaactgaaaa
    46801 acttccgtca gctgcactct aaaactccgg gccacccgga agtgggttac accgctggtg
    46861 tggaaaccac caccggtccg ctggggcagg gtattgccaa cgcagtaggt atggcgattg
    46921 cagaaaaaac gctggcggcg cagtttaacc gtccgggcca cgacattgtc gatcactaca
    46981 cctacgcctt catgggcgac ggctgcatga tggaaggcat ctcccacgaa gtttgctctc
    47041 tggcgggtac gctgaagctg ggtaaactga ttgcgttcta cgatgacaac ggtatctcta
    47101 ttgatggtca cgttgaaggc tggttcaccg acgacaccgc aatgcgtttc gaagcttacg
    47161 gctggcacgt tattcgcgac atcgacggtc atgacgcagc atccatcaaa cgcgcagtag
    47221 aagaagcgcg cgcagtgact gacaaaccgt ccctgctgat gtgcaaaacc atcatcggtt
    47281 tcggttcccc gaacaaagcc ggtactcacg actcccacgg tgcgccgctg ggcgacgctg
    47341 aaattgccct gacccgcgaa cagctgggct ggaaatacgc gccgttcgaa atcccgtctg
    47401 aaatctatgc tcagtgggat gcgaaagaag caggtcaggc gaaagaatcc gcatggaacg
    47461 agaaattcgc agcttacgcg aaagcttatc cgcaggaagc cgctgaattt acccgccgta
    47521 tgaaaggcga aatgccgtct gacttcgacg ccaaagcgaa agagttcatt gctaaactgc
    47581 aggctaaccc ggcgaaaatc gccagccgta aagcgtcgca gaatgctatc gaagcgttcg
    47641 gcccgctgtt gccggaattc ctcggcggct ccgctgacct ggcaccgtct aacctgaccc
    47701 tgtggtctgg ttctaaagca atcaacgaag atgctgcggg taactacatc cactacggtg
    47761 ttcgcgagtt cggtatgacc gcgattgcta acggtatctc cctgcacggt ggcttcctgc
    47821 cgtacacctc caccttcctg atgttcgtag aatacgcacg taatgccgta cgtatggctg
    47881 cgctgatgaa acagcgtcag gtgatggttt acacccacga ctccatcggt ctgggcgaag
    47941 atggcccgac tcaccagccg gtagagcagg ttgcttctct gcgcgtgacc ccgaacatgt
    48001 ctacatggcg tccatgtgac caggttgaat ctgcagtggc atggaaatac ggtgttgagc
    48061 gtcaggacgg cccgaccgcg cttatcctct cccgtcagaa cctggcgcag caggagcgta
    48121 ctgaagagca gctggcaaac atcgcgcgcg gtggttatgt gctgaaagac tgtgccggtc
    48181 agccggaact gatcttcatc gcgaccggtt cggaagttga actggctgtt gccgcctacg
    48241 aaaaactgac tgccgaaggc gtgaaggcgc gcgtggtttc catgccgtct accgatgcat
    48301 tcgacaagca ggatgcggct taccgtgaat ccgtactgcc gaaagcggtt actgcacgcg
    48361 ttgcggtaga agcgggtatt gctgactact ggtacaagta tgttggcctg aacggtgcta
    48421 tcgtcggtat gaccaccttc ggtgaatctg ctccggcaga gctgctgttt gaagagttcg
    48481 gcttcactgt tgataacgtt gttgcgaaag caaaagcgct gctgtaatca gtaagacgtt
    48541 ttgtgagaag ggccgcgaaa gcggcccttt agattattgg taaagtgcgc gttgttcatg
    48601 tcggatgcgg cgtgaacgcc ttatccgacc tacaagatcg tgcaaattca atatattgca
    48661 ggaagtatgt aggcctgata agcgtagcgc atcaggcagt tttgcttttg ccatcactct
    48721 caagggccgc gaaagcggcc ctttttttac ttagtagaga taaacggaac tgtgctgccg
    48781 gggatcatgg tatcgggcag tttaccgtcc caacgttcgg cagtggtgag ggctacaagt
    48841 ccgggattat cccgcagagc ttcaccgcgc aggcggattg cttctgcttc agccgcactt
    48901 tttaaacgaa tagtttcagc ctctgccgcg cctcttacgc gaatagtttc cgcttctgct
    48961 ttggctgccg ccagtttact gtctgcttct gcctgggcct gagttaccgc tatttgcgcc
    49021 tggattttct ctgtttccag attttgctta cgcgtggcaa tggcgacttc tgctttcatt
    49081 cggtcttcaa tcgacttttc ataagcgtca gaaaaatcaa tattttcaat ctggaccccg
    49141 tcaattacca ctggcccaac aacggctttg cgcatggcat tttgcagatc ctgaaccagt
    49201 tttgtacgat cctgtacagc gctgatggcg gtatattggc cgaaaacgtt ctccagttga
    49261 gttggcaact gacggacaat cagacgatct tttaatgctt caatggtgtt gtaggtggta
    49321 taaaccgcgc ctgcttctga aggtttaatg tggaagctga ctgatactgt catttgtgcc
    49381 ggttgctggt cgcggctgta ggcctgtaat ccctgataga ccacagcctg attacgcgtg
    49441 gaaattttct ccacgctttc cataaacggg atcttaaaac ccagacccgg ctcggcaact
    49501 tttacgattt ttccgtagcg cagcaaaatg ccgcgttcac cttcattaac ggtgtaatac
    49561 gagagaaatg gcagcaaaac cacggcaagc acgccgatgg cgatagcaag agatttttgt
    49621 gggcgaaatg aggttaatga aacaggtgct ttcatgacaa tcttccttga gtaaagtgtg
    49681 ggaatatgtc acatgagatg aggagaaaac agccgacagg tgagaattca gattctataa
    49741 aggggtatag cgataaaaca ttgcattaga aaagtatgtt ttcgaacatt caataaataa
    49801 atagagatat gcatcacaaa tacaataaca aataaaatgt tgattttgtg ataaacataa
    49861 caaatacaaa gttgacttta aaaatatcaa tttcatattg ttgttattta aggttttatt
    49921 attttggttt atggggtttt tcttctcgcg tacccaattg ctttcattaa accgcacgag
    49981 tattttattt ttaccgccgg acggtaccct aaaaataaaa taaggataat ttatgcggct
    50041 tagtgattat tttccagaat catcaatctc agttatacat tcagcaaaag attggcagga
    50101 agctatcgat ttctcgatgg tatcattgct ggataaaaac tatatcagcg agaattacat
    50161 tcaggcaatt aaagattcca ccattaacaa tggcccttat tatattctcg caccaggcgt
    50221 ggcaatgcct catgcgcgac cggaatgtgg ggcgcttaaa accgggatgt ctttgacatt
    50281 acttgaacaa ggtgtttatt ttccggggaa tgatgaacca attaaattac tcatcggact
    50341 ctctgctacc gatgccgatt cgcacattgg cgctattcag gcgttaagtg aattactgtg
    50401 cgaagaagaa atactcgaac aactcttaac agcatcatca gaaaaaaaat tagcggacat
    50461 tatcagccgc ggataattac tctctcctct acaggataat attatggaaa acaagtctgc
    50521 tcgtgcaaag gtccaggctt ttgggggctt tttgactgca atggtcatcc ccaatattgg
    50581 tgcttttatt gcctggggtt ttattactgc gttatttatt cccaccggtt ggctgcctaa
    50641 cgaacatttc gccaaaattg tcggcccgat gattacctat ttattgcccg tgatgattgg
    50701 ttctaccggt ggtcatctgg tcggcggtaa acgcggcgcg gtcatgggcg gaataggtac
    50761 tattggtgtg atcgtcggcg cagagatccc gatgttcctt ggctcaatga ttatggggcc
    50821 gctcggtggt ctggtcataa aatatgttga taaggcactg gaaaaacgca tacctgccgg
    50881 ttttgagatg gttatcaata acttctcatt aggtatcgcg gggatgctcc tttgcctgct
    50941 gggttttgaa gtgatcggcc cggcggtgtt aattgccaat actttcgtca aagagtgtat
    51001 tgaggcgctg gtacatgcgg gttatctgcc tctgctgtca gtcatcaatg aaccggcgaa
    51061 agtgcttttc cttaataatg cgatcgatca gggcgtctat tatccgctgg gaatgcaaca
    51121 ggcttcggtt aacggtaaat ccatcttctt tatggtggcc tctaacccag gtccgggcct
    51181 ggggctgctg ctggcgttta ccttgtttgg taaagggatg agtaaacgtt ctgcgcccgg
    51241 ggcgatgatt attcacttcc tcggtgggat ccacgaactg tatttcccat atgtgctgat
    51301 gaagccgctg actattattg ccatgattgc gggcggtatg tctggcacct ggatgtttaa
    51361 cttactggac ggtggtctgg tggctggccc aagtccgggg tctatctttg cttacctggc
    51421 gctgacgccg aaaggttcgt tcctggcgac aattgccggt gttacggtag gtaccctggt
    51481 gtcctttgct atcacttcgc tgatactgaa gatggaaaaa acggtggaaa cggagagcga
    51541 agatgagttt gctcagtcag ccaatgcggt taaggcgatg aaacaagagg gtgcattctc
    51601 gttaagcagg gttaagcgta tcgcctttgt ttgcgatgcg gggatgggct ccagtgcgat
    51661 gggcgcgacc accttccgta aacgcctgga aaaagcgggg ctggcaattg aagtaaaaca
    51721 ttacgccata gaaaacgtgc ctgcggatgc ggatatcgtc gttactcatg ccagtctgga
    51781 agggcgcgtg aaacgtgtga cggataaacc actgatattg attaataact atattggcga
    51841 tccaaaactc gacactttat ttaatcaatt aaccgccgaa cataaacact gattacagag
    51901 gtaaaaatga aaaccaaagt tgctgctatt tatggcaagc gggatgtccg tctgcgcgaa
    51961 tttgaactgc cagaaattac cgataatgaa ttactggtga gtgtgatttc tgacagcgtc
    52021 tgtttatcca cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
    52081 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
    52141 aaaaatctca ctggaaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
    52201 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
    52261 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
    52321 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
    52381 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
    52441 gcactgctgg cgtgtgcagg tccgatgggc attggcgcta tcgattacgc cattaacggc
    52501 ggcatacaac cgtcgcgggt ggtggtggtc gatatcgacg acaaacgtct ggcgcaggta
    52561 cagaagctgc tgccggtgga actggcggcc agtaaaggca ttgagctggt gtatgtgaat
    52621 accaaaggga tgagcgatcc tgtccagatg ctgcgggcgc tgacaggaga tgccgggttc
    52681 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga tgaattactg
    52741 gcggaagatg gctgtctgaa cttctttgcc gggccgacgg ataaaaactt caaagtgccg
    52801 tttaatttct acaacgttca ttacaacagc acgcacgtcg tcggtacatc cggcggttca
    52861 acggacgaca tgaaagaggc gattgccctt agcgccactg ggcagttaca gccgtcgttt
    52921 atggtgaccc atatcggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
    52981 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
    53041 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
    53101 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
    53161 gatattgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
    53221 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
    53281 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
    53341 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
    53401 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
    53461 tggggcagag caatgcgctg gcggtcatgg ctttcgcccc tcgagatagc ctgttacatg
    53521 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
    53581 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
    53641 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaggaagcaa
    53701 cacagctggg ggtgaaagtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
    53761 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccggaaggcg
    53821 tgatttctgc ctgtgcagtc aaagcgttag gcggagatat gcaggcggaa ttaatcgatt
    53881 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
    53941 gttgcaaggc aatgggcgtt gaagtgaacc gtgtgtactc gctcgacgaa ctggtgaggg
    54001 ggaacgatat cctctttagc gctacgggcg tcacgggcgg cgagctggtg aacggtatcc
    54061 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
    54121 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
    54181 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaata
    54241 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
    54301 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
    54361 acgatattga tgttgcactg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
    54421 acgccgatat cacgcatttt tcccagtact ggcattacct gaaagaacag gatgaaacgc
    54481 tcggatttgc cgatgacatg acttgggatt ttatcagcaa cgtcaatagc ataacctgca
    54541 atgcgacgct ctatgacgcg ttgaaagcga tgaagttcgc cgatttcgcc gtctggtcag
    54601 aggcgcgttt tagcgggatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
    54661 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
    54721 ccatgatgaa gagataaaac gcgtgcataa accgttgctg cgtatgctgg cagccttaca
    54781 gacggtaaat ccgcagcggc gtactgtggt ttttctttgc gctccgccgg ggacggggaa
    54841 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggaac tgcccgctat
    54901 tcagacgctg ccgatggatg gttttcacca ttacaatagc tggctggatg cgcatcaatt
    54961 gcgccccttc aaaggcgcac cagagacatt tgacgtagcg aaactggcgg aaaatctgcg
    55021 ccaggtcgtg gaaggggatt gtacgtggcc gcagtatgat cgacaaaagc atgatcctgt
    55081 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
    55141 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaatgc
    55201 gccagctact gcgttgcggg aacgactggt ggggcgcaag ctggcgggag ggttatcgct
    55261 ggcagacgca gaagcttttt atgaccgtac cgacgggccg aatgttcgac gggtgctgga
    55321 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
    55381 tgattaacgt tcctgattag accatcttcc agtcgtacca cacgagagaa atgacgccgg
    55441 gtaaatgcgg cgtcatgact gattgctacg acgctcgtgc ctcgctggcg gcacttctcc
    55501 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
    55561 agcaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
    55621 ctatgcagat ccaatgggtg cgcgctggca gtgtctgcta actgacaaca ttgcagggca
    55681 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
    55741 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
    55801 atctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
    55861 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
    55921 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
    55981 agacaatccg ttgccgcgcc aggccagcga taagatatct gatttaacgt taacatagtg
    56041 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
    56101 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
    56161 gcaacagcat ttcgctggca tgcggcgtca ggcggctaaa ggcttcatca agaattaata
    56221 gctttggctg catcgcgatg gcgcaggcga tgaccacgcg ctgtgtctct ccaccggaga
    56281 gtgttgctgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
    56341 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
    56401 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
    56461 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
    56521 tgcctgttat ctctcctggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
    56581 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
    56641 agtcataaca gaacggagga tgcgttgtgt gtgtcgggca ataacggaac tgttctaatg
    56701 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
    56761 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
    56821 tgcccgcata tccagcgccg caccacggac agcaagatcg tttagcgcat tgtgtgttag
    56881 cggaataatt aacgcgggca tcgcacgtaa acgctgatac cagccctcat ccagcggcac
    56941 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
    57001 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
    57061 gcggataaaa cattgcacgg ggacgtactg catccacaac tgtgaggttg aaacaatcgc
    57121 cagaatccgc aaccagagcg taatagcatg cgtccaacgc tccggcgaac gcggcgtgcc
    57181 gcttagccat tctgttagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
    57241 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcaaaa
    57301 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
    57361 ggccgccagc gcccacagcg ttaaggatgt aaacggatgc atttagcgca cggcagccat
    57421 tgccggaaaa tggcgcgtgg tgcaaatcgg cagctggcgc agtaaaagcc agacaatcac
    57481 cgctgtaaga attttatcga ccaggtttgc gcctatcacc gtaatcgcca ccgactccac
    57541 caggttttgc cccatagagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
    57601 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
    57661 cacgccgctg acaacgactt ttggcagggt gcggaaccaa cccgctcgcg ccagccaacc
    57721 ggcgaccagg ccgatgacca tcgccacggg tgcaaatgcg gcggcgatcg ggtcggtcag
    57781 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
    57841 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
    57901 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
    57961 ggaaaagtgg ctacgcgcca taagaaaacc tctctgaaag tagttattta acgggggaaa
    58021 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
    58081 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
    58141 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
    58201 acgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
    58261 cggcatagcg caaggcgatg tcagtcaggc taccggtaca gcctgcaatc caggcggcat
    58321 gaagttgctg ctgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
    58381 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgggta gggcaatgtc atcattggag
    58441 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
    58501 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
    58561 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgtt
    58621 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
    58681 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
    58741 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
    58801 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
    58861 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
    58921 gcggatgctg cgggcatggc gcatttgttg aaatacgaca ccagccatgg ccgttttgca
    58981 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
    59041 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
    59101 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc acattgccgc cggggctaaa
    59161 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
    59221 aatcaggatc aacttcgttc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
    59281 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
    59341 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
    59401 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgccggt
    59461 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
    59521 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
    59581 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
    59641 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
    59701 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
    59761 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
    59821 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
    59881 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
    59941 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
    60001 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
    60061 tcctaccgaa ggcgagtaca acgaagaatt ctctcttctg ccggttgtta actacctgaa
    60121 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
    60181 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
    60241 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
    60301 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
    60361 cgcaggcccg ctgctggcag ctgaactcga cgcgctgggt aaagcactga aagaacctgc
    60421 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
    60481 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
    60541 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
    60601 aaaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
    60661 gttctctgaa accgctccgg ctaccctgaa atctgttaac gacgtgaaag ctgacgagca
    60721 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
    60781 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
    60841 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
    60901 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
    60961 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
    61021 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcccttgt ttcagcgcgc
    61081 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
    61141 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
    61201 catgcaactt gaagtatgac gagtataagg cccgacgata caggacaaga gacatgtcta
    61261 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
    61321 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
    61381 tcaacgccgt actggaaacc gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
    61441 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
    61501 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
    61561 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
    61621 tgctggacgc gggtgaaaaa cacttcgctg ctaccggcaa gccgctgttc tcttctcaca
    61681 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
    61741 agcgcatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
    61801 aagacggcgt ggacaacagt cacatggacg cttctgcact gtacacccag ccggaagacg
    61861 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gcagcgtcct
    61921 tcggtaacgt acacggtgtt tacaagccgg gtaacgtagt tctgactccg accatcctgc
    61981 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    62041 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    62101 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    62161 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    62221 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    62281 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    62341 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag aaaaattgcc
    62401 tgatgcgcta cgcttatcag gcctacacgt atcctgcaat atattgaatt cgcaggtttt
    62461 tgtaggccgg ataaggcgtt cacgccgcat ccggcatgac aaagcgcaca ttgatatctg
    62521 tctgagcccc gctctgcggg gattttttcg ccaaaagcaa ggtgatacag atgagaaatc
    62581 tgtgatctat ttggcaaaat tatgctttat tgtttaccct tgtcagactg cccgtcataa
    62641 ggcggcggag tgtatttctc cattttgagt cagttgaaaa ggaatattga atggaagatt
    62701 tgaatgttgt cgatagcata aacggcgcgg gaagctggct ggtggctaac caggcgctgc
    62761 tgctaagtta tgcagtaaac atcgtggcgg cactcgcgat catcatcgtt ggtttgatta
    62821 tcgcgcggat gatttccaac gcggtgaatc gcctgatgat ctcccgtaaa atcgatgcca
    62881 ctgttgctga ttttctttct gcattagtcc gttacggtat tatcgccttt acgctaatcg
    62941 ctgcactggg acgcgtgggt gtacaaaccg cgtccgtcat tgctgtactc ggtgccgcag
    63001 gcttagctgt tggtctggct ttgcaggggt cactgtctaa cctggccgct ggcgtgttac
    63061 ttgtcatgtt ccgcccgttc cgtgccggag aatatgttga cctcggcggc gtagccggta
    63121 ctgtgctgag tgtgcagatt ttctccacca ccatgcgtac tgcagacggt aaaattatcg
    63181 ttattccgaa cggtaaaatt attgccggaa atattattaa cttctcccgc gagccagttc
    63241 gccgtaacga gtttattatt ggcgtggcgt atgattccga tatcgatcag gttaagcaga
    63301 tcctgaccaa tattatccag tctgaagatc gcattttgaa agatcgcgaa atgactgtgc
    63361 gcctgaacga acttggtgca tcgtcgatta atttcgtggt ccgcgtctgg agcaacagtg
    63421 gcgatctgca aaacgtgtac tgggatgtgc tggagcgtat taaacgtgaa tttgatgccg
    63481 ccggtatcag cttcccgtac ccgcaaatgg atgtgaactt taagcgggtg aaagaagaca
    63541 aagctgcgta atcaacgctg aggccagata atactctctg gcctctctct tattagtttt
    63601 tctgattgcc aattaatatt atcaatttcc gctaataaca atcccgcgat atagtctctg
    63661 catcagatac ttaattcgga ataccaaacg tgttttctta ttactttcaa ggtcttgcgc
    63721 ttggcgcagc tatgatccta ccgctcggtc cacaaaatgc ttttgtgatg aatcagggca
    63781 ttcgtcgtca gtaccacatt atgattgcct tactttgcgc tatcagcgat ttggtcctga
    63841 tttgcgccgg gatttttggt ggcagcgcgt tattgatgca gtcgccgtgg ttgctggcgc
    63901 tggtcacctg gggcggcgta gccttcttgc tctggtatgg ttttggcgct tttaaaacag
    63961 cgatgagcag taatattgag ttagccagtg ccgaagtcct gaagcaagga agatggaaaa
    64021 ttatcgccac catgttggca gtgacctggc tgaatccgca tgtttacctg gatacttttg
    64081 ttgtactggg cagccttggc ggtcagcttg acgtcgaacc aaaacgttgg tttgcgctcg
    64141 ggacaattag cgcctctttc ctgtggttct ttggtctggc tattctcgca gcctggctgg
    64201 caccgcgcct gcgcacggca aaatcacagc gcattatcaa tctggtagtg ggatgtgtca
    64261 tgtggtttat agccttgcag ctggcgagag acggtattgc tcatgcacaa gccttgttca
    64321 gttaggcgct atctgatgga aaaataaaac agaggcgcta agcttgcctc cagaggtcct
    64381 gaattttagg caagtcaaat aacacggaga gactaacgtg aagttcaaag ttatcgccct
    64441 ggcggcatta atgggtatta gcgggatggc agcgcaggct aacgaattgc cggatggacc
    64501 gcatattgtc acctccggta cggcaagcgt ggatgcggtg ccagacattg ccactcttgc
    64561 gattgaagtc aatgtggccg caaaggatgc cgctactgcc aagaaacagg cggatgagcg
    64621 cgtcgcacaa tacatttcct tccttgaact caatcagatc gcgaaaaaag atatcagctc
    64681 agcgaactta cgcacccagc cagattatga ttatcaggat ggtaaaagta tccttaaagg
    64741 ctaccgcgct gtgagaacgg tggaagtcac gctccgtcag ttagacaaac tgaattcctt
    64801 gctggatggc gcgctgaagg cgggtcttaa cgaaattcgt tctgtgtcgc tgggcgtggc
    64861 gcagccggat gcctataaag acaaagcacg taaggcagcg attgataacg cgattcatca
    64921 ggcgcaggaa ctggcgaacg gctttcatcg taaactgggg ccggtatata gcgtgcgcta
    64981 ccatgtttcc aactatcagc ccagcccaat ggtgcggatg atgaaagccg atgccgcgcc
    65041 ggtctcagcc caggaaactt acgagcaggc cgctattcag tttgatgatc aggtcgatgt
    65101 ggtcttccag ttagaacctg tggatcaaca acccgctaaa acacctgcag cacaataata
    65161 cttgtagccc gacgtattcg tcgggctaat gcctttaccc gatattgcct cctttttctg
    65221 taattttctg taaaaccatg acgtaatcac ctatctttgc tcgcggtttc atcaaggata
    65281 ggctgactga aacggttaag cggacaagga tgcgggggag gggggatgga catttttatt
    65341 tcgaaaaaga tgcgcaattt cattttatta gcgcaaacca ataatattgc ccgggcggca
    65401 gaaaaaatcc atatgacggc ttcgccattt ggtaaaagca ttgccgcgct ggaagaacaa
    65461 attggctata cgctatttac ccgcaaagat aataatatca gcctcaataa agccggacag
    65521 gaactgtatc aaaaactgtt cccggtttat caacggcttt ctgcgattga taatgaaatc
    65581 cataactcag ggcgtcgttc acgagaaatt gtgattggca tagataatac ttatccgaca
    65641 attattttcg atcagttgat tagtctcgga gataagtatg aaggcgtgac cgcccagcca
    65701 gttgagttca gcgaaaacgg cgttatcgat aatctttttg accgccagtt agattttatt
    65761 atctccccac agcatgtatc tgcacgagtt caggaactgg aaaacctgac tatcagtgaa
    65821 ctaccaccgc tgcgattggg ctttctcgtt tctcgccgtt atgaagagcg tcaagagcaa
    65881 gagctgcttc aggagctgcc ctggctacaa atgcgctttc aaaaccgggc taacttcgaa
    65941 gcaatgattg atgcgaatat gcgaccttgt gggatcaatc caaccattat ttatcgtccc
    66001 tacagtttta tggccaaaat cagcgccgtg gagcgcggac attttctgac ggtgatcccg
    66061 cattttgcct ggcgactggt gaatccggca acgctgaaat attttgatgc gccgcacagg
    66121 ccgatgtata tgcaggaata tctttattca atcagaaatc atcgttatac cgccacgatg
    66181 cttcagcata ttgctgaaga tcgtgacggg acaaaccatt aacccagcat cgagccggtt
    66241 gcaattaaat tacggtgtaa gtcgaagacg tggctaagat cgtggtgaat atgtccgcca
    66301 ggcgcatttt ccagatagcg atgcagataa tcccgataca taggatgtgc acaattatca
    66361 atgatcgtgc gggcgcgttg aagcggggaa agaccgcgca gatcggcgat cccttgttcg
    66421 gtgatgatca ctttgacgct gtgttcgctg tgatcaacat ggctgcacat tggcacgacg
    66481 gttgagatct tgccttcttt agcaatcgat ggggccataa agatcgacag atacgcgttg
    66541 cgttcaaaat caccgctgcc gccgatgccg ttcatcagat cgaccccggc tacgtgcgtt
    66601 gagttggcat gcccgtaaat atcaaactcc aggccgacgt tcagagcgat gacgcccaga
    66661 cgacggatga tttccgggtt attggaaatc tcctgcggac gcaacacaat gcggctggca
    66721 aagtaatcca tattgtcgta aatcttgcgc agggaatcgg ccgagattgt caggctggag
    66781 gcgctggccc cgctgatttt gccggtttcc agtaaatgca ccaccgattc ctgtagcact
    66841 tccgaataca tcataaacgg aggaatttcc gggttttccc ccagacgcgc cattaccgca
    66901 ttattgatat tgcccacgcc actttgcagc ggcagaaatt ccggcggaat acgcccatgc
    66961 gccatttcct gcaataagaa cgtgaccacg ttatcggcaa tctgctggca catgggattt
    67021 tgcttatcca gcatattacc ggcgtcgggc aagttggttt ccacgacggc gacaatcttt
    67081 ttcggatcga tttgcacata gcgggtaccg acgcgatcca ttgcatggaa gatcgacacg
    67141 ctattacgcc gcggtggcgc gccaggaatc acaatatctg ccagttctgc aacgcgcgga
    67201 tcgtgatagt gattaagttc aatgatcact ttcttcgccc gcagcagcca tgtcggcgca
    67261 ttaccgatcc cgctggttaa ccagactcga ccatccggtg ccagtgccga tgcttcaatg
    67321 acggcaacat caatgtcgcc gaagaaaccg taattgacca tttgcgccac ttcgctcaaa
    67381 tgcaggtcaa cgaaactcac cgcgccctga ttgatctttt tacgtaaacc ggacgatgtt
    67441 tgatatggcg cacgccagga aacagcatca gcgtcagaaa gtacatcgtc agcggcggcg
    67501 ctgattgacg cacccgtcag caggcgaatt tgatacggct ttttggcctc atgctgttcg
    67561 ttagctctgc gggcaatcgc ggtgggtagg gctttcggtg aacccgctgg ggtaaagccg
    67621 ctaaatgcca ccatgtcgtt atgctggata atttctgccg cttcatcggc ggtcatcctt
    67681 gtccactgag tttccatttt tactccttcg ttcgcaggga ttaatgacca acgaaattag
    67741 gtttacgttt ttcgaggaaa gcgttcatcc cttcctggta atcttcgctg tcataaacgg
    67801 cgcggcgcat cccctgaata cgttcaaatt catcggagtt catggtgtgt gcttcgccca
    67861 gtacacgcag ctcttctttg ataacggcaa tggctaacgg cgctttctca gagatgtggt
    67921 gcgccatttg taaggtgaaa tcttccagtt cttccacttc cacaacatgg ttgaggatgc
    67981 cgacagccag cgcgcgctgg gcggtgattg gcgaagcggt aaaaatcagc tctttgacaa
    68041 tgtggaagcc cgcgtcgcgg gtcaggttgt gaatgccgac caggttatac gggacgccga
    68101 ggtttacagg tgtcattgag aaggttgagg tactggcggc gatgatcaga tcggaactca
    68161 tgatcatttc aaatgcgcca ccccaaacac taccttccac catcgaaatg atcggtttcg
    68221 ggaatttttg gatcatgcgg gtgatttgac gcaacggatc atcataggag agcggatcgc
    68281 gaccgccaga cggcagttcg tgaatatcgt gacctgcgga gaagactttg gatccactcg
    68341 gcgcgcgcaa aatgatacag cgaatttccg gtcggttgag atcgcttaat gcctgcataa
    68401 gatcatcaat aaagacttta cttaaggcat taagtttgcg gccatagtta aactcaatga
    68461 ccgccacttt gttgatagtg acaacgttca catactgata agacataaaa attcctttaa
    68521 tcaaaatatt gcgtctggat aaattcactg agctgccgca ggccggtgcg cggtgagagc
    68581 gtattgtttt tgaccgctaa aagcgtctgg cggtaatagc gatcgaaatc ttcattcgcg
    68641 aacaggtgat tcagtacttc ttcttcggtt tgtttacgca gccattccac cgattgttgt
    68701 tgccgcactt gctgtaaacg accactggca gttagcgcgg ttttgaagtc gatgatggcg
    68761 tgccagatct catcgattcc acgtttttcc agtgcgctac aagtcagaac ccgtggctgc
    68821 cattcgtcgt atttacgtcg cagaatatgc agggcactct cgtacatatg ccgggcaatg
    68881 gcgacattgg tatggttatc gccatcgtct ttgttgataa cgatcagatc agccacttcc
    68941 atcagccctt ttttaatgcc ctgcagatca tcgccgccac cggcaatttg caacgagata
    69001 aaacagtcca ccatgcgggc gacttccgtt tccgactgcc cgacgccaac cgtttcgaca
    69061 atcactacgt cataacccgc cgcttcgcat aacagcatta attcccgcgc tcgctgactg
    69121 gcaccgccca gatgaccgga ggatggtacc gggcgaataa acgccgtctc ggcacgcgcc
    69181 aggtcattca tgcgggtttt atccccgaga atgctaccgc cagtgaccgg actgctggga
    69241 tcgaccgcaa taaccgcaac ttttaatcca tcacgaatca acaacatgcc aaaggcttca
    69301 agaaaggtac ttttccccgc gccgggtgtg ccggtaacgc ccagtcgcag ggcgttaccg
    69361 cagtacggca taatggcatc aagcagttgc gtacttagcg cctgatgacg cgggtgacgg
    69421 ctttccacca gcgtcatggc ctgggcgagt gtggcacgct caccctgacg taagcggcga
    69481 atactttctg ccagcgtggc ttcattaatc atgatgctgg cttatcagat tcagtacgtc
    69541 gcgcacactg tcgagcatag gtgtacctgg accataaatc gccgccacgc cgcgctcttg
    69601 caggaaggcg taatcctgcg gtggaatgac gccaccggcg actacgcaga tatcttcgcg
    69661 tccccatttt ttcagcgctt cgaccagttc cgggatcagc gttttatgac cggcagccag
    69721 tgaggatgcg cccactacgt gaacgtcatt ttctactgcc aggcgggcga tctcttcagg
    69781 tgtagagaac atcgggctta aatctacgtc gaaaccgaga tcggaatagg cgctggcgat
    69841 cacttttgcg ccgcgatcgt gcccatcctg gcccattttg gcgatcagaa tgcgcgggcg
    69901 acgaccattg tcggcaagga actgctccgt ttgcgcaaca atggcatcga actcggaggc
    69961 cgatttctca gattgatgat agctttgcgc aattacgccg gtaacacact ggcttggcac
    70021 cagataacgg tcgaaagcgg cttccagcgc atcggaaatt tcacccaggg tggcgcgaac
    70081 gcgagcggca ttaacagcgg cagccagcag gttttcgtta tgctgtgcgg cgtgagtcag
    70141 ggcgttcaac gcggcggtta cggcggcatc atcacgggtg gcgcgaatgc gttccagcga
    70201 agcaatttgc tcgttacgca ccatcacgtt gtcgatctca agtacatcgg tttcgtcttc
    70261 gtgatccagt ttgtacttgt tgacaccaac gatgacacgc ttgccctggt cgatcagcga
    70321 ctgttcgcgc gctgaggcct cttcgatcat tcgttttggc agacctgctt cgatcgcttt
    70381 cgccatgcca ccggcttcgt cgatctgttg gataataact ctggcttgtt tgacgatttg
    70441 atcggtcagc gactcaatgt aataggatcc ggccagtgga tcgacggtgc ggcagagttc
    70501 tgattcttcc tggatgatga tctgggtgtt gcgggcaatg cgtgctgaga aatcggtagg
    70561 caaaccaagc gcttcgtcaa aggcgttggt atgcagtgac tgagtaccgc ccagcgtcgc
    70621 agccagtgct tcaatggtgg tgcggataac gttgttatac ggatcctgtt cagtcaggct
    70681 ccagcctgag gtctggcagt gggtacgcag cgccagtgat ttcgggtcct gtgcgccaaa
    70741 tccactgacc gcttcgctcc ataaataacg tgccgcacgc aacatggcga cgttcataaa
    70801 cagatccatg ccgatgccga agaagaacga caggcgagga gcgaagtcat caattttcag
    70861 tccggcagag attgctgctt tgatgtactc aatcccatca gcgagcgtaa atgctacctg
    70921 ctgcacgcag ttggcacccg cttcacccat gtggtaaccg ctgatactga tggtattaaa
    70981 tcgcggcatg ttgccggaac accaggcgat gatgtcggcg ataatgcgca ttgacggttt
    71041 tggcgggtaa atataggtgt tgcggcagag gtactctttg aggatatcgt tctgaatggt
    71101 gccggtcagt ttatcaggtg taacaccttg ctcttccgcg gcgacgatat aaaacgccag
    71161 tactggtagc actgcgccat tcatggtcat cgaaaccgac attttatcca gcgggatctg
    71221 gtcgaacagg actttcatat cttccacggt gtcgatagcg acgcccgctt tgccgacgtc
    71281 gcccgccacg cgcgggttat cggagtcgta gcctcggtgg gtggcaaggt caaacgcaac
    71341 ggaaagacct ttttgcccgg cggccaggtt acggcgataa aaagcgttgg actcttttgc
    71401 tgttgaaaaa ccagcatact gacggatggt ccacggttgg gcggtataca tagtggcacg
    71461 cgggccacga acgtagggcg gcaaaccagg aagggtacct gtcacctcca gattatcgag
    71521 atcggcttcg gtatacagcg gcttgatggc gatcccttcc gcggtttgct gaaccagcga
    71581 gtcgacagtt ttctcccgac ggctcaattc cttgttggca agctgttgcc actcctgcac
    71641 gttagacatt atttgctccg taaaacgcaa tcaaaatgag ggtatttgcg cctacggtga
    71701 gctttttacg tgggtttgtc gccagcaaaa aaaatgcatt cagacgtcgt tttttgccgc
    71761 ctgagatttg tctattttgt gatgtgattc aaggaataca aaaagaagtg aggacaacgc
    71821 ctgatatgtg ctatcaggca ttatttgatg gattaatcct gacgaaggac tttgtgacca
    71881 taatcgagta acgcatcagt gactttacgc atcatgcggc tttcaggagc aaagcggtgc
    71941 cagtagagca tccgtcgttg aaatagccca ggcgttaagt caatcagttc accgctggcc
    72001 agctctttct cgatttgcag gtgcgggatc atacagcagg tggtgccctg gcgagcaagt
    72061 tgcacgaacg cttctgaaga attaacgata tggcagggta cgctgccagg aggcagatcg
    72121 aagttttgct gcaaaaaggc ctggtgcata tcgtcaagat ggtcaaacgc gaccactggc
    72181 gctttcagta atgccgaacg cgttacgccg ttagggaaat atttttcggc aaacggtttt
    72241 gagctgacga acagatagtc gagcgcacca agtttatcga caagacaact cggcagcgcc
    72301 tgatgttgaa tactcaccgc gccgaccact tcgccgcgac gcagacgttc ctgagtgcgg
    72361 gtttcatctt ctacctgcaa gttgaggcgg ataggcgaat cagccaacac aggagccagt
    72421 gccggaagca accacgtcgc cagactgtcg gcgttgaccg ccagtgaaag cagcagcgga
    72481 gtcgaaccgg tttgttcatc gcccagccac tcttcttcca gcaactccac ctggcgcagc
    72541 agtgccagca gtttttgccc ttgttccgtc gggcgcggcg gtacggtacg caccaacagc
    72601 ggctgcccga acatattttc cagttgctta atgcgctgtg agacggctga ttgtgtaatg
    72661 cacagctttt gtgccgcgcg ctcaaatcct cgttcacgta tcaccgcatc cagtgcctgt
    72721 aatgttctgt agtccgggcg tttcattgct gttgtctgct cctggtaaat gctttttcct
    72781 gcactatgac ataattttgc gttgggtaca gagcatttaa cgcatacaaa agcggtcgta
    72841 atggaagttt tggctggcaa tcccattttc atataaccct ttgctgagcg ctttggcaaa
    72901 actctgtggg ccgcaaaacc agaaatgggt gtcaccttta tttgctattt tatcagtaag
    72961 atagtgcgac tgtaggcgct cacctttatg cgcatcaagc agatgcagtg tgacactggc
    73021 ctgggcacaa agtgaggtaa gtttatcaac ataccatgca tcctcgcttc ggctgacgca
    73081 ataccagaga tccacaggaa cacgggacgc gccatgctgg ttcagatact caagctgggc
    73141 gataaaaggg gtaatgccga tgcctcctgc tatccagacc tggcgggagc aagggagagt
    73201 aaaatcaaac tttccccatg gcccttcaac gagggcggac tggccgattt gtatcgagga
    73261 tgccagagtc gaggtataat cccctaaatt tttgatggca aagcgcaacg tgttcttctc
    73321 ttttacatat gaagcgatag taaaagggtg agattcttta ctgtgtgcga acgttaagaa
    73381 tgcaaactgc cccggacaat agtcgatggg atgttccatc tccagtatga cttcggtaat
    73441 ttgtcccgga tggttaacaa tgcttttaac ggtggcctga tgcttgcttt tattaccgtt
    73501 taatttcagc agcgaaatga aagccacaaa gacgcctggg atagtaatta aaattaataa
    73561 atacgcagca ggagtttccc accaatatga tttaaacagt gcagtaaata catgaaaagc
    73621 aattgccaga taaaaaacgg ggaacaaacg gtgaacaaga tgaaataaat gatagggaac
    73681 ttttttgctt agagacacga aaaccatcgc tatcataata aacattgcac attcacccgc
    73741 taacaatccc gcatgaagcc aattttcttg tactggcgtc aggttaatat caacgatttt
    73801 accagggtga gtaagccagc caaaggcgac gaataactgg ggtaattttt catccagcca
    73861 gtgggcgaaa gcaaaagcga tggcgcatat tcctgcccac ttatgtaatc gccatgcttt
    73921 atccagaccg cccagcactt tattaagcca tgatgaacgc agcgataaac acatcgtcag
    73981 cgtcatcaga caccacgcaa gggttcccgt aaaaaagagc gtgttttgtc gaatgaacca
    74041 tattagcgcg attggctttt cacgaaaaat atcgctggac gacaaaaaaa taatacatgc
    74101 gacaaagcaa accaacgtac ctgtaagatt agcttttttc attttttatt tcattttggc
    74161 agcggagcat agaacagttc catgctcccg ttaaatagag tgattattta tagaaaatca
    74221 cagtggcgtg aatataattt ttaccggaga aagaacttat ataataattg ctggcttgta
    74281 actcttccgc tttctctttg atttggcttt caacgcctgc tgggttgcta ttgtgtgtag
    74341 acatgctcac ttttgataac tgttcaaggt tattgtccgg tttatgtatc tcggttgcgg
    74401 caaatgcgtg tcctgtgaga gtgcaaagaa ataacgcggc aaataaattt tgcttaatag
    74461 acataataac tccttttata ttaaagataa tgattaaata cttgtgtaat aagttcaggg
    74521 atttgtgcta atgcattttg ctcggcgagt tcgcgtcccg gttgggtttt gtcgacgcct
    74581 tccgcacgta aaatatgtgt attggtaatg cccattaatt tcagcgtggc ttgcaggtaa
    74641 ctttcctgat gatccagcgc cagatgtgct tcatcgcaat agataccacc gcgcgtcgag
    74701 acaataaacg tcggtttatc cgtcagtaac ccaacggcac cagcggaagt aaaacgaaac
    74761 gttaaaccag cctgacagac ctgatctatc caggttttga ggccagatga aacgctgtga
    74821 ttaatcatcg gcgcgccaat aatgagcaca tcgcacgatg ccagttcttc aatagcggcg
    74881 tgaatcccat tcacctcctg ccgaatttgt gcgctcccgc actctgcttt atcgcaaagc
    74941 gccatgaagg tttcttcacg taaatggggc ggcggcgtca ggccaacgtc gcgatgaata
    75001 attgtggctt tcggaaagtg ttggcaaagc gcttctacca gcgtctggct taatttccgc
    75061 gaacttgaat gcttaagcat tgggctactg tcgatatgaa gaattttcat gggcttgctc
    75121 tcatttctgc gatgagagaa gtttaactgt tgtttatatg gtgattaaat agacataatt
    75181 gaacttcatt gttgtgaaaa tagaacaatc atgatcgact ggaatgatta ttactacttt
    75241 gccctgattg ccgatgaagg cagctacagc caggcggcgc tgcgtgcggg cgtgtcgaaa
    75301 tcggtattaa gccgacgaat ggctgcactg gaggagcgcc ttggcgtacg gcttattcag
    75361 cgaactacgc ggcgtctggc cctgacggcg gcaggcgaac agtttgctgt cgaatgtcgg
    75421 gcgctgatgg cgcaggagga acgggccaga gcggtggtga tggccgccca ggaaagccca
    75481 cagggaatgc tgcgcgtcag cgcgccggtg tttatggctg aaacatggtt tggtgagttt
    75541 gtcagtgagt ttatcgcccg ttggcccctg gcgaaggtgc aactgctggc aattaatcgc
    75601 ccggtggata tggtcgctga aggcattgat attgcgctgg ttacggcttc cggcgcgctt
    75661 gctgactcga cgctgcatta taaaaaatta catacccagg ctgatatttt agttgccagt
    75721 tctcagtggc tggcccgcca tccgcaggtg aaagctattg aagatcttaa ccacgtcgac
    75781 gggatccttc ggctggcgaa caatggcggg agccagtggc aattaattaa tggcgaacag
    75841 gagattacgc tgcagcctga ggcgcgtttg cagtcaaata accttcgcgt catgctgcaa
    75901 gcagtacttg gtgggaatgg cgtggcgtta ttgcctcaat ttgcctgtaa acaggggctt
    75961 gcgaacggtg aactggtgcg cctgtttgca ccgtggagcg gcatacccag accgttgtat
    76021 gctttatttg cggggcgaaa ggggatgcct gccattgcgc gatattttat ggatgagtta
    76081 accacgcggc ttgccaacgg ggtctgaatc gctttttttg tatataatgc gtgtgaaatt
    76141 tcataccaca ggcgaaacga tcatgacgca ggatgaattg aaaaaagcag taggatgggc
    76201 ggcacttcag tatgttcagc ccggcaccat tgttggtgta ggtacaggtt ccaccgccgc
    76261 acactttatt gacgcgctcg gtacaatgaa aggccagatt gaaggggccg tttccagttc
    76321 agatgcttcc actgaaaaac tgaaaagcct cggcattcac gtttttgatc tcaacgaagt
    76381 cgacagcctt ggcatctacg ttgatggcgc agatgaaatc aacggccaca tgcaaatgat
    76441 caaaggcggc ggcgcggcgc tgacccgtga aaaaatcatt gcttcggttg cagaaaaatt
    76501 tatctgtatt gcagacgctt ccaagcaggt tgatattctg ggtaaattcc cgctgccagt
    76561 agaagttatc ccgatggcac gtagtgcagt ggcgcgccag ctggtgaaac tgggcggtcg
    76621 tccggaatac cgtcagggcg tggtgaccga taatggcaac gtgatcctcg acgtccacgg
    76681 catggaaatc cttgacccga tagcgatgga aaacgccata aatgcgattc ctggcgtggt
    76741 gactgttggc ttgtttgcta accgtggcgc ggacgttgcg ctgattggca cacctgacgg
    76801 tgtcaaaacc attgtgaaat gatctgacgg gggaacctcc cccgttaaaa aaattctctt
    76861 cattaaattt ggtgacatgt gtcacgtttt tacctggcta ttgtcgattg ctctaaataa
    76921 atcctctaaa ccagcatatt catccaagaa ttacctttgc gtgatatttc ctcaacatcg
    76981 cgacgcaaac gttcatattg ccgcaatatt attttttgat atgttgaaag gcggacgcaa
    77041 atccgcacac aacatttcaa aagacaggat tgggtaaatg gcaaaggtat cgctggagaa
    77101 agacaagatt aagtttctgc tggtagaagg agtgcaccaa aaggcgctgg aaagccttcg
    77161 tgcagctggt tacaccaaca tcgaatttca caaaggcgcg ctggatgatg aacaattaaa
    77221 agaatccatc cgcgatgccc acttcatcgg cctgcgatcc cgtacccatc taactgaaga
    77281 cgtgatcaac gccgcagaaa agctggtcgc tattggctgt ttctgtatag gaacaaacca
    77341 ggttgatctg gatgcggcgg caaagcgcgg gatcccggta tttaacgcac cgttctcaaa
    77401 tacgcgctct gttgcggagc tggtgattgg cgaactgctg ctgctattgc gcggcgtgcc
    77461 ggaagccaat gctaaagcgc accgtggcgt gtggaacaaa ctggcggcgg gttcttttga
    77521 agcgcgcggc aaaaagctgg gtatcatcgg ctacggtcat attggtacgc aattgggcat
    77581 tctggctgaa tcgctgggaa tgtatgttta cttttatgat attgaaaata aactgccgct
    77641 gggcaacgcc actcaggtac agcatctttc tgacctgctg aatatgagcg atgtggtgag
    77701 tctgcatgta ccagagaatc cgtccaccaa aaatatgatg ggcgcgaaag aaatttcact
    77761 aatgaagccc ggctcgctgc tgattaatgc ttcgcgcggt actgtggtgg atattccggc
    77821 gctgtgtgat gcgctggcga gcaaacatct ggcgggggcg gcaatcgacg tattcccgac
    77881 ggaaccggcg accaatagcg atccatttac ctctccgctg tgtgaattcg acaacgtcct
    77941 tctgacgcca cacattggcg gttcgactca ggaagcgcag gagaatattg gcctggaagt
    78001 tgcgggtaaa ttgatcaagt attctgacaa tggctcaacg ctctctgcgg tgaacttccc
    78061 ggaagtctcg ctgccactgc acggtgggcg tcgtctgatg cacatccacg aaaaccgtcc
    78121 gggcgtgcta actgcgctga acaaaatctt cgccgagcag ggcgtcaaca tcgccgcgca
    78181 atatctacaa acttccgccc agatgggtta tgtggttatt gatattgaag ccgacgaaga
    78241 cgttgccgaa aaagcgctgc aggcaatgaa agctattccg ggtaccattc gcgcccgtct
    78301 gctgtactaa taccccttct ctgaaaatca acgggcaggt cactgacttg cccgtttttt
    78361 tatcccttct ccacaccgtt gcgcattcta ctttgcgagt atgctccaga aaataaatca
    78421 aagtcagatc aatgcgttgt gtttatttaa tgggg
//
LOCUS       DAZFKF010000010        77423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFKF010000010 DAZFKF010000000
VERSION     DAZFKF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 77423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.010"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1068)
                     /locus_tag="ACHKA1_000836"
     CDS             complement(<1..1068)
                     /locus_tag="ACHKA1_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO5678534.1"
                     /translation="MDYRFSWVIFNMINNISDQVSSFPCTQLNQSDNFLDSLREFFAI
                     LNSSRKGELSTWDTIYLHLILAINADSDLIKNDVLLAENIPTADYQFNTFFSNAFETD
                     VKKYLGKSEDNEVEIKTGNERISIGIRNISNGKLERQKFLFPLDYENKLQHQLDKHFT
                     IESHPLLYRHTMGSKIANVIFEKLYSRIDFNKEQYISFIKDAFLHFYDYSRRYAINEN
                     IAKDAVTNNIALMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFET
                     KPVHYQDLLTRFSAFQDTVFNLFPEMHSSHYNDICSVSVDMTKGTQCMIHLMVNEEVF
                     MSLPVPVATMVREDATNLINLK"
     gene            1692..2207
                     /gene="ybjP"
                     /locus_tag="ACHKA1_000837"
     CDS             1692..2207
                     /gene="ybjP"
                     /locus_tag="ACHKA1_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5678535.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            2425..3153
                     /gene="artP"
                     /locus_tag="ACHKA1_000838"
     CDS             2425..3153
                     /gene="artP"
                     /locus_tag="ACHKA1_000838"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO5678536.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            3171..3902
                     /gene="artI"
                     /locus_tag="ACHKA1_000839"
     CDS             3171..3902
                     /gene="artI"
                     /locus_tag="ACHKA1_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO5678537.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            3909..4625
                     /gene="artQ"
                     /locus_tag="ACHKA1_000840"
     CDS             3909..4625
                     /gene="artQ"
                     /locus_tag="ACHKA1_000840"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO5678538.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            4625..5293
                     /gene="artM"
                     /locus_tag="ACHKA1_000841"
     CDS             4625..5293
                     /gene="artM"
                     /locus_tag="ACHKA1_000841"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO5678539.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            5584..6315
                     /gene="artJ"
                     /locus_tag="ACHKA1_000842"
     CDS             5584..6315
                     /gene="artJ"
                     /locus_tag="ACHKA1_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO5678540.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKISDQWFPQ"
     gene            complement(6366..7847)
                     /locus_tag="ACHKA1_000843"
     CDS             complement(6366..7847)
                     /locus_tag="ACHKA1_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFO5678541.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GGNYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQIETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWDPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSSAMFGVFSGHANVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(7859..8179)
                     /locus_tag="ACHKA1_000844"
     CDS             complement(7859..8179)
                     /locus_tag="ACHKA1_000844"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO5678542.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHFPH
                     LINQ"
     gene            complement(8201..9544)
                     /locus_tag="ACHKA1_000845"
     CDS             complement(8201..9544)
                     /locus_tag="ACHKA1_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO5678543.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
     gene            complement(9824..10951)
                     /gene="rlmC"
                     /locus_tag="ACHKA1_000846"
     CDS             complement(9824..10951)
                     /gene="rlmC"
                     /locus_tag="ACHKA1_000846"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO5678544.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM"
     gene            complement(10992..11480)
                     /gene="ybjO"
                     /locus_tag="ACHKA1_000847"
     CDS             complement(10992..11480)
                     /gene="ybjO"
                     /locus_tag="ACHKA1_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO5678545.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(11540..12385)
                     /gene="potI"
                     /locus_tag="ACHKA1_000848"
     CDS             complement(11540..12385)
                     /gene="potI"
                     /locus_tag="ACHKA1_000848"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO5678546.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPAERGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(12382..13335)
                     /gene="potH"
                     /locus_tag="ACHKA1_000849"
     CDS             complement(12382..13335)
                     /gene="potH"
                     /locus_tag="ACHKA1_000849"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO5678547.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMNHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQFSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(13345..14478)
                     /gene="potG"
                     /locus_tag="ACHKA1_000850"
     CDS             complement(13345..14478)
                     /gene="potG"
                     /locus_tag="ACHKA1_000850"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO5678548.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(14573..15685)
                     /gene="potF"
                     /locus_tag="ACHKA1_000851"
     CDS             complement(14573..15685)
                     /gene="potF"
                     /locus_tag="ACHKA1_000851"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO5678549.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(16036..16512)
                     /gene="ybjN"
                     /locus_tag="ACHKA1_000852"
     CDS             complement(16036..16512)
                     /gene="ybjN"
                     /locus_tag="ACHKA1_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO5678550.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(16600..17502)
                     /gene="rimK"
                     /locus_tag="ACHKA1_000853"
     CDS             complement(16600..17502)
                     /gene="rimK"
                     /locus_tag="ACHKA1_000853"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO5678551.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(17563..18285)
                     /gene="nfsA"
                     /locus_tag="ACHKA1_000854"
     CDS             complement(17563..18285)
                     /gene="nfsA"
                     /locus_tag="ACHKA1_000854"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO5678552.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(18269..18556)
                     /gene="ybjC"
                     /locus_tag="ACHKA1_000855"
     CDS             complement(18269..18556)
                     /gene="ybjC"
                     /locus_tag="ACHKA1_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO5678553.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            18716..18973
                     /gene="grxA"
                     /locus_tag="ACHKA1_000856"
     CDS             18716..18973
                     /gene="grxA"
                     /locus_tag="ACHKA1_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO5678554.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(19003..19380)
                     /gene="ybjM"
                     /locus_tag="ACHKA1_000857"
     CDS             complement(19003..19380)
                     /gene="ybjM"
                     /locus_tag="ACHKA1_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO5678555.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            19650..21335
                     /gene="ybjL"
                     /locus_tag="ACHKA1_000858"
     CDS             19650..21335
                     /gene="ybjL"
                     /locus_tag="ACHKA1_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO5678556.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(21510..22046)
                     /gene="rcdA"
                     /locus_tag="ACHKA1_000859"
     CDS             complement(21510..22046)
                     /gene="rcdA"
                     /locus_tag="ACHKA1_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO5678557.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVIDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            22130..23338
                     /gene="ybjJ"
                     /locus_tag="ACHKA1_000860"
     CDS             22130..23338
                     /gene="ybjJ"
                     /locus_tag="ACHKA1_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5678558.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            23338..24153
                     /gene="ybjI"
                     /locus_tag="ACHKA1_000861"
     CDS             23338..24153
                     /gene="ybjI"
                     /locus_tag="ACHKA1_000861"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO5678559.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQDIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            24239..24523
                     /gene="ybjH"
                     /locus_tag="ACHKA1_000862"
     CDS             24239..24523
                     /gene="ybjH"
                     /locus_tag="ACHKA1_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO5678560.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(24564..25796)
                     /gene="mdfA"
                     /locus_tag="ACHKA1_000863"
     CDS             complement(24564..25796)
                     /gene="mdfA"
                     /locus_tag="ACHKA1_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO5678561.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            26081..26677
                     /gene="ybjG"
                     /locus_tag="ACHKA1_000864"
     CDS             26081..26677
                     /gene="ybjG"
                     /locus_tag="ACHKA1_000864"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO5678562.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQATGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            26735..27493
                     /gene="deoR"
                     /locus_tag="ACHKA1_000865"
     CDS             26735..27493
                     /gene="deoR"
                     /locus_tag="ACHKA1_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO5678563.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(27540..28742)
                     /gene="dacC"
                     /locus_tag="ACHKA1_000866"
     CDS             complement(27540..28742)
                     /gene="dacC"
                     /locus_tag="ACHKA1_000866"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO5678564.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            28989..29615
                     /gene="gstB"
                     /locus_tag="ACHKA1_000867"
     CDS             28989..29615
                     /gene="gstB"
                     /locus_tag="ACHKA1_000867"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO5678565.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDTLFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(29612..30727)
                     /gene="yliI"
                     /locus_tag="ACHKA1_000868"
     CDS             complement(29612..30727)
                     /gene="yliI"
                     /locus_tag="ACHKA1_000868"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO5678566.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(30838..31221)
                     /gene="bssR"
                     /locus_tag="ACHKA1_000869"
     CDS             complement(30838..31221)
                     /gene="bssR"
                     /locus_tag="ACHKA1_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO5678567.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            31434..32759
                     /gene="rimO"
                     /locus_tag="ACHKA1_000870"
     CDS             31434..32759
                     /gene="rimO"
                     /locus_tag="ACHKA1_000870"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO5678568.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(32806..34134)
                     /gene="dgcI"
                     /locus_tag="ACHKA1_000871"
     CDS             complement(32806..34134)
                     /gene="dgcI"
                     /locus_tag="ACHKA1_000871"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5678569.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(34142..36490)
                     /gene="pdeI"
                     /locus_tag="ACHKA1_000872"
     CDS             complement(34142..36490)
                     /gene="pdeI"
                     /locus_tag="ACHKA1_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5678570.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYLLTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(36667..37578)
                     /gene="gsiD"
                     /locus_tag="ACHKA1_000873"
     CDS             complement(36667..37578)
                     /gene="gsiD"
                     /locus_tag="ACHKA1_000873"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO5678571.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIMVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(37581..38501)
                     /gene="gsiC"
                     /locus_tag="ACHKA1_000874"
     CDS             complement(37581..38501)
                     /gene="gsiC"
                     /locus_tag="ACHKA1_000874"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO5678572.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(38519..40057)
                     /gene="gsiB"
                     /locus_tag="ACHKA1_000875"
     CDS             complement(38519..40057)
                     /gene="gsiB"
                     /locus_tag="ACHKA1_000875"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO5678573.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFNTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(40077..41948)
                     /gene="gsiA"
                     /locus_tag="ACHKA1_000876"
     CDS             complement(40077..41948)
                     /gene="gsiA"
                     /locus_tag="ACHKA1_000876"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO5678574.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQVLRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAVARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAPVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(41935..42900)
                     /gene="iaaA"
                     /locus_tag="ACHKA1_000877"
     CDS             complement(41935..42900)
                     /gene="iaaA"
                     /locus_tag="ACHKA1_000877"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO5678575.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSSHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDAVATQ"
     gene            43104..44339
                     /gene="moeA"
                     /locus_tag="ACHKA1_000878"
     CDS             43104..44339
                     /gene="moeA"
                     /locus_tag="ACHKA1_000878"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO5678576.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            44339..45088
                     /gene="moeB"
                     /locus_tag="ACHKA1_000879"
     CDS             44339..45088
                     /gene="moeB"
                     /locus_tag="ACHKA1_000879"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO5678577.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALARINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(45164..45826)
                     /gene="fsa"
                     /locus_tag="ACHKA1_000880"
     CDS             complement(45164..45826)
                     /gene="fsa"
                     /locus_tag="ACHKA1_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO5678578.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMAITAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            45957..46856
                     /gene="ybiY"
                     /locus_tag="ACHKA1_000881"
     CDS             45957..46856
                     /gene="ybiY"
                     /locus_tag="ACHKA1_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO5678579.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            46862..49294
                     /gene="ybiW"
                     /locus_tag="ACHKA1_000882"
     CDS             46862..49294
                     /gene="ybiW"
                     /locus_tag="ACHKA1_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO5678580.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRFHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            49440..50255
                     /gene="ybiV"
                     /locus_tag="ACHKA1_000883"
     CDS             49440..50255
                     /gene="ybiV"
                     /locus_tag="ACHKA1_000883"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO5678581.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            50402..51667
                     /gene="ybiU"
                     /locus_tag="ACHKA1_000884"
     CDS             50402..51667
                     /gene="ybiU"
                     /locus_tag="ACHKA1_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO5678582.1"
                     /translation="MAPTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLSGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPDDELCGVAPGKVLPISEKWHPLLLKALTSIPAITAG
                     DSVWWHCDVIHSVAPVDNQQGWGNVMYIPAAPMCEKNRAYAHKVKMALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(51908..53500)
                     /gene="ybiT"
                     /locus_tag="ACHKA1_000885"
     CDS             complement(51908..53500)
                     /gene="ybiT"
                     /locus_tag="ACHKA1_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO5678583.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            53719..54639
                     /gene="ldtB"
                     /locus_tag="ACHKA1_000886"
     CDS             53719..54639
                     /gene="ldtB"
                     /locus_tag="ACHKA1_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5678584.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(54697..55815)
                     /gene="ybiR"
                     /locus_tag="ACHKA1_000887"
     CDS             complement(54697..55815)
                     /gene="ybiR"
                     /locus_tag="ACHKA1_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO5678585.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAVGFALLARRVVLSVDWTLLLVFMAMFIDVHLLIQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(55812..56279)
                     /gene="mntR"
                     /locus_tag="ACHKA1_000888"
     CDS             complement(55812..56279)
                     /gene="mntR"
                     /locus_tag="ACHKA1_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO5678586.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            56465..56593
                     /gene="mntS"
                     /locus_tag="ACHKA1_000889"
     CDS             56465..56593
                     /gene="mntS"
                     /locus_tag="ACHKA1_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO5678587.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            56865..58448
                     /gene="opgE"
                     /locus_tag="ACHKA1_000890"
     CDS             56865..58448
                     /gene="opgE"
                     /locus_tag="ACHKA1_000890"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO5678588.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKDGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(58498..59013)
                     /gene="ompX"
                     /locus_tag="ACHKA1_000891"
     CDS             complement(58498..59013)
                     /gene="ompX"
                     /locus_tag="ACHKA1_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO5678589.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(59066..59131)
                     /locus_tag="ACHKA1_000892"
     CDS             complement(59066..59131)
                     /locus_tag="ACHKA1_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678590.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            59366..60253
                     /gene="rhtA"
                     /locus_tag="ACHKA1_000893"
     CDS             59366..60253
                     /gene="rhtA"
                     /locus_tag="ACHKA1_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO5678591.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            60552..61055
                     /gene="dps"
                     /locus_tag="ACHKA1_000894"
                     /gene_synonym="pexB"
     CDS             60552..61055
                     /gene="dps"
                     /locus_tag="ACHKA1_000894"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO5678592.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            61459..62205
                     /gene="glnH"
                     /locus_tag="ACHKA1_000895"
     CDS             61459..62205
                     /gene="glnH"
                     /locus_tag="ACHKA1_000895"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO5678593.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            62344..63003
                     /gene="glnP"
                     /locus_tag="ACHKA1_000896"
     CDS             62344..63003
                     /gene="glnP"
                     /locus_tag="ACHKA1_000896"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO5678594.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            63000..63722
                     /gene="glnQ"
                     /locus_tag="ACHKA1_000897"
     CDS             63000..63722
                     /gene="glnQ"
                     /locus_tag="ACHKA1_000897"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO5678595.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            63983..66208
                     /gene="ybiO"
                     /locus_tag="ACHKA1_000898"
                     /pseudo
     CDS             63983..66208
                     /gene="ybiO"
                     /locus_tag="ACHKA1_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(66205..67131)
                     /gene="rlmF"
                     /locus_tag="ACHKA1_000899"
     CDS             complement(66205..67131)
                     /gene="rlmF"
                     /locus_tag="ACHKA1_000899"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO5678596.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            67407..67667
                     /gene="mcbA"
                     /locus_tag="ACHKA1_000900"
     CDS             67407..67667
                     /gene="mcbA"
                     /locus_tag="ACHKA1_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO5678597.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            67932..70214
                     /gene="fiu"
                     /locus_tag="ACHKA1_000901"
     CDS             67932..70214
                     /gene="fiu"
                     /locus_tag="ACHKA1_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO5678598.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAARLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            70256..70933
                     /gene="ybiX"
                     /locus_tag="ACHKA1_000902"
     CDS             70256..70933
                     /gene="ybiX"
                     /locus_tag="ACHKA1_000902"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO5678599.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            71007..71273
                     /gene="ybiI"
                     /locus_tag="ACHKA1_000903"
     CDS             71007..71273
                     /gene="ybiI"
                     /locus_tag="ACHKA1_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO5678600.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            71538..71798
                     /gene="ybiJ"
                     /locus_tag="ACHKA1_000904"
     CDS             71538..71798
                     /gene="ybiJ"
                     /locus_tag="ACHKA1_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO5678601.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(71901..71960)
                     /gene="ybiE"
                     /locus_tag="ACHKA1_000905"
     CDS             complement(71901..71960)
                     /gene="ybiE"
                     /locus_tag="ACHKA1_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO5678602.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(72027..73112)
                     /gene="hcxB"
                     /locus_tag="ACHKA1_000906"
     CDS             complement(72027..73112)
                     /gene="hcxB"
                     /locus_tag="ACHKA1_000906"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO5678603.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDGGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(73253..74215)
                     /gene="ybiB"
                     /locus_tag="ACHKA1_000907"
     CDS             complement(73253..74215)
                     /gene="ybiB"
                     /locus_tag="ACHKA1_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO5678604.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            complement(74243..76393)
                     /gene="dinG"
                     /locus_tag="ACHKA1_000908"
     CDS             complement(74243..76393)
                     /gene="dinG"
                     /locus_tag="ACHKA1_000908"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO5678605.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDMLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            complement(76677..>77423)
                     /gene="espX2"
                     /locus_tag="ACHKA1_000909"
     CDS             complement(76677..>77423)
                     /gene="espX2"
                     /locus_tag="ACHKA1_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFO5678606.1"
                     /translation="LSHQALISMLEPEVKKKTIWNNYSIYPSLQDTHEVVRDDPETIC
                     TRAFPLFAKGWEYAQRNKKHQLILNALGFKGYIRDIFMSAIMRKTDFVPECNNQPTEL
                     NSSFSSLMNDSDQWQQHTLKDKHYASLLTMLDLNEASESDKSKIFFCLSAVFANISHS
                     NVFNGIPDASKTLKRYAFALLAKAHSLDESMISNQTFNTYKTVLLDFNNLSNEEANQL
                     RISSLYRDMVRYAQYRFSKVLSEWTPDAWL"
BASE COUNT        19444 a        20288 c        18706 g        18985 t
ORIGIN      
        1 tttaagattg attaaattag ttgcatcttc acgtaccatc gtggcaacag gaacaggcaa
       61 tgacatgaaa acttcttcat tcaccatcaa gtgaatcatg cattgcgttc cctttgtcat
      121 atccacactt acgctacaaa tatcgttgta atgcgaagag tgcatttcag gaaacagatt
      181 aaacacggta tcttgaaagg cactaaatcg tgttaataaa tcctgataat gaacaggttt
      241 tgtttcaaaa ttatcatcag caaatccaag cactattgca tgctctacat ctaacgcagt
      301 ttcaaatgac tcttcttctg taaaatcatt atttaaaacc tcacctgacg tattatcact
      361 gtcataaaaa gtgctcatca atgcgatatt gtttgttaca gcgtcttttg caatattctc
      421 attaattgca tatcgcctgc tataatcata aaaatgaaga aacgcgtcct ttataaagga
      481 tatatattgt tctttgttaa agtcaatacg actgtataat ttttcaaaaa tcacattcgc
      541 aatcttacta cccatagtat gcctgtataa taatgggtgc gattcaatcg taaaatgctt
      601 gtctaattga tgttgtaatt tattctcata gtccagtggg aaaagaaact tttgtctttc
      661 aagttttcca ttcgatatgt tacgtattcc aatagagatt ctttcattac ccgtttttat
      721 ttcaacttcg ttatcttctg atttacccag atatttttta acatcagtct cgaaagcatt
      781 actaaaaaat gtattaaatt gataatccgc tgttggaata ttttccgcga gcaacacatc
      841 attttttatg agatcactgt cagcatttat tgctaatatc agatgcaagt agatggtatc
      901 ccatgtagac aactcaccct tacgactgga atttaatatc gcgaaaaatt ccctcagtga
      961 atccaggaaa ttatcacttt gattcaattg tgtgcatgga aatgatgaca cttgatcaga
     1021 tatattgttt atcatattaa aaatcaccca agaaaaacga taatccaccg aactcctagc
     1081 gctttattat tgtaaaagca caacaccttt tatactcata ttaattaacg aaacactgaa
     1141 acaacttgat ttattcttcg ctaaaattca cttgttcaaa aatattatat tttataccca
     1201 caactgtagc ataaataata ttatcaaaag tcaaagctgg gactatcaaa tttcgctcat
     1261 tttcaaaaat acaacgtaat tttggcaatc atcttaatag caggatctct tatcgcatgt
     1321 gaaaaatttg taatgattag acaataaccg cgttcattta tcacgcgtta taaatgtgaa
     1381 actggcttct tgcagatgct cttaacctct acaggaggat ttataccacc atttcagaac
     1441 tatcatattt accttaaatg atcatttcag ccatataaca cgctcaacct cacaaatggt
     1501 ttacactgtc tattgtgtgg gtaatttaag taagtcagct acgagcaacc ttgagtgggt
     1561 agccagcaat ggactcaaac ccaatcaggt gttcagattg tttcagaatt gtattaaaaa
     1621 caatgtatta aataatctga tagcgtttgg tcgtagtgtg acgcagacag caagctaata
     1681 aggaaatgat tatgcgctac cgttcactat ctctacttat tccatgtgca ctgctgctga
     1741 gtgcctgtac caccgtcacg ccagcttata aagataacgg tccccgcacc ggttcctgtg
     1801 tccagggggg gcctgacagc gtggcccaac aattctatga ctaccgcatt caacatcgca
     1861 gcaatgacat taccgccctg cgcccctatt tgagtgacaa actggcaacc ctacttagcg
     1921 atgccagccg cgataatagt catcgtgaat tgttaagcag cgatccgttc tccagtcgaa
     1981 ccaccttgcc agacagcgcc cacgttgcca gtgcatcgac tatccctaat cgtgatgccc
     2041 gtaacattcc attgcgcgtg gatctcaagc agggtgacca gggctggcag gatgaagtgt
     2101 taatgattca ggaaggccag tgttgggtga tcgacgatgt gcgctactta ggcggtagcg
     2161 ttcatgccac ggcgggtacg ctccgtcagt ccatcgagaa tcgctaacgc cttgatcaac
     2221 caataaaccc cgtaaattgt ccggcatttc gtgaggaatg ccgacattta tgctcgccga
     2281 ccaccgcccc cgttattttg tgctatgttt attgaataat gcgctttgct tttaactttt
     2341 aaagcagaaa tattgcataa ttattctgtc aaaggtacta tcttcgacct caatagctat
     2401 cagactgcca gtatacgagt gtcaatgagt attcaattaa acggcattaa ttgcttctac
     2461 ggcgcgcatc aggcgctgtt cgatatcacg ctggattgcc cacagggcga aacgctggtg
     2521 ttacttggcc ccagcggcgc gggtaaaagc tcgctgctgc gtgtactcaa tctgcttgag
     2581 atgccgcgct ccggtacgct caacattgca ggcaaccatt tcgatttcac caaaacaccc
     2641 tcagacaaag cgattcgcga tttgcgtcgt aacgttggca tggtgtttca gcaatacaac
     2701 ctgtggccgc atctgaccgt gcagcaaaac ctgattgaag cgccctgccg tgtactgggg
     2761 ttgagtaaag atcaggcgct ggcccgtgca gaaaaactgc tggaacgtct gcgtctcaaa
     2821 ccttatagcg atcgttaccc gctgcatctt tctggtggtc agcagcagcg tgttgctatt
     2881 gcccgtgcgt taatgatgga accgcaggta ctgctgtttg atgaaccgac cgccgcactg
     2941 gacccggaaa ttaccgcaca aatcgtcagc atcattcgtg agctggcaga aacgaatatt
     3001 acccaggtga tcgtcaccca cgaagttgaa gtggcgcgta aaaccgccag ccgagtggtg
     3061 tatatggaaa atggtcatat cgtagaacaa ggcgacgcga gctgctttac cgagccgcaa
     3121 accgaagcat ttaaaaacta tctctctcac taagattcgg gataacaaca atgaaaaaag
     3181 ttctgattgc cgcgttaatt gcaggtttta gtctttccgc cacagctgcc gaaaccattc
     3241 gttttgctac cgaagcctcc tatcctccgt ttgaatcgat tgatgcaaac aaccagatcg
     3301 ttggttttga cgtcgacctg gcacaggcac tgtgtaaaga gattgatgca acctgtactt
     3361 tctctaacca ggcgtttgac agcctgatcc caagcctgaa attccgtcgc gtagaagccg
     3421 tgatggcggg catggatatc actccggagc gtgaaaagca ggtgctgttt accaccccgt
     3481 actatgacaa ctctgccctg tttgtgggtc agcaaggcaa atacaccagt gttgatcagc
     3541 tgaaaggcaa aaaagtcggc gttcagaacg ggacgacaca ccagaagttc attatggata
     3601 agcacccgga aatcaccacc gttccgtatg acagctacca gaacgcaaaa ctggatctgc
     3661 aaaatggtcg tatcgacagc gtatttggtg acaccgcagt ggtaaccgaa tggctgaaag
     3721 ataacccgaa actggcggca gtgggcgata aagtgaccga taaagattac ttcggtactg
     3781 gcctcggcat cgcggtacgt cagggcaaca ctgaactgca gcagaaactc aacactgcgc
     3841 tggaaaaagt gaagaaagat ggcacttacg aaaccatcta caacaaatgg ttccagaagt
     3901 aattcctgat gaatgaattt tttcctttag caagcgccgc cgggatgacc gtcggccttg
     3961 ccgtttgtgc attgattgtc ggactggcgc tggcgatgtt ctttgcggtg tgggagtcgg
     4021 caaaatggcg tcctgtcgcg tgggcaggtt cagcgctggt aaccattcta cgtggcctgc
     4081 cagaaattct ggtggtgctg tttatctatt ttggctcctc acagctgctg ctgacgcttt
     4141 cggatggctt caccatcaat cttgggttcg tgcagatccc ggtgcagatg gacattgaga
     4201 acttcgacgt gagtccgttc ctttgtggtg tcatcgctct gtcactgctg tatgccgcct
     4261 atgcctcgca aacgctgcgc ggcgcgttga aagcggtacc ggtgggccag tgggaatctg
     4321 gtcaggcgct ggggctgtcg aaatcggcta tctttttccg tctggtgatg ccgcagatgt
     4381 ggcgtcatgc gctgcctggc ctcggtaacc agtggctggt gctgctgaaa gataccgcgc
     4441 tggtcagttt gattagtgtg aatgatttaa tgctacaaac caaaagcatc gctactcgta
     4501 cccaggaacc atttacctgg tacattgtgg cggcggcgat ttacctggtg atcaccctgc
     4561 tcagtcagta cattctcaaa cgcattgacc tgcgcgcgac acgttttgag cggaggccca
     4621 gctaatgttt gagtatttac ccgaactgat gaaagggctg cacaccagcc tgacgctaac
     4681 cgttgcctcg ctgattgtgg cactgattct ggcgttgatt tttaccatca tcctgacgct
     4741 gaaaacgccg gtgctggtgt ggctggtgcg gggttatatc acgctgttta ccggtacacc
     4801 gctgctggtg cagatcttcc tgatttatta cgggccgggt cagttcccga cgttgcagga
     4861 gtatccggca ctgtggcatt tgttgtcaga accgtggtta tgtgcgctga ttgcgttgtc
     4921 gctgaacagt gcggcgtata ccacgcagct gttttacggt gcaattcgtg cgatcccaga
     4981 aggtcagtgg cagtcctgta gcgccctggg aatgagcaaa aaagatacgc tggcgatcct
     5041 gctgccgtat gcctttaaac gctcgctctc ttcttattcc aacgaagtgg tgctggtgtt
     5101 caaaagtacc tctctggctt acaccattac gctgatggaa gtgatgggat acagccagtt
     5161 gttgtacgga cgcacctacg atgtaatggt gttcggtgcg gcagggatta tttacctggt
     5221 cgttaacggc ctgctgacgc tgatgatgcg tctgatcgag cgcaaagcac tggcattcga
     5281 acggcggaat taatgtagct gcgtacattc gccggggggt ttgtaggtct gataagacgc
     5341 gccagcgtcg catcagacat cagcacggtg cctgtgccgg atgcggcgtg gatgtcttat
     5401 ccggcctaca tagtgcataa attcttataa taaagacgga caacttagtg agttgtccgt
     5461 ctttttttat ttcatgcaaa ttatttaatc atatttattg catataaatt cacttgatgg
     5521 cattgttatc ccatgccgca gacacggcca aaaatcataa gattgacaga cgggagttcc
     5581 atcatgaaaa agttagttct tgccgcatta cttgcttcct ttactttcgg tgcttctgcc
     5641 gcagagaaaa tcaattttgg cgtttcagcc acctatccac cctttgaatc tataggtgct
     5701 aataatgaga ttgtcggctt tgatatcgac ctggcaaaag ccttgtgcaa acaaatgcag
     5761 gcagaatgta cttttactaa tcacgctttc gacagcctga tcccgtcact gaaattcagg
     5821 aaatatgacg ccgtaatctc cggtatggat atcacaccag atcgtagcaa gcaggtatcg
     5881 ttcaccacgc cctattacga aaactcagcc gtcgtgattg ccaaaaaaga tacctacaaa
     5941 acgtttgccg acctgaaagg caaacgcatt gggatggaaa acggtactac gcaccagaaa
     6001 tatattcagg atcagcaccc ggaagtgaaa acggtctcct atgacagtta tcaaaatgcc
     6061 tttatcgatc tgaaaaatgg ccgtattgat ggcgtatttg gtgacactgc ggtggttaat
     6121 gaatggctga aaaccaatcc gcaactgggc gttgctactg aaaaagtgac cgatccgcaa
     6181 tactttggca ccggtctggg tatcgctgta cgtccggata acaaagctct gctggaaaaa
     6241 ctgaataacg cgctggcagc aattaaagct gacggtacat atcagaaaat cagcgatcag
     6301 tggttcccgc aataattatt catttgccgg agtgggcgaa cacccactcc ggcaaaatca
     6361 cttctctacg ctaatcccag gcgctgaaac tgctctggcg gcgcatcatt atctttcatc
     6421 aactgaatca gcaacgcgat catccgggac tcgatcacat catcatgaat cggatgtagc
     6481 tgctgcggat cgttttgcag atcaaacagc aaatgaccaa actgattcaa ccccttatat
     6541 ttttcctgtg ccgggatgcg taatacctgt aaccctttgg taaaacgaaa cggtggggcc
     6601 agcgataaag cccgcatctc atccaccgga tagcggctgt tcattttgca ggacatcaac
     6661 gtgtagttgg caatatcatc ctcacgcccc ggcagcgctg cacgcatata cacataacgc
     6721 ccatcagtta cgttagcatg accactgaaa acaccaaaca tcgccgagct gcgtactggt
     6781 tcatcgtgac tgaccgtttt cgccagcggt tgcccctgca catcgggcgg cactggctgt
     6841 tgaaaaaagt catacaacgt tggcgcccag tcgatcattt gtaccagtgc ctgtcgccgc
     6901 tctcctttta caccactgcg cgggtcccag ataaacaacg gattattggc aacttcgttg
     6961 taataaggca tctggttttt cgcccaccag ccatgctcac cgagtaaaaa gccgtggtca
     7021 gtgccgacaa tcagcatggt atctcgccac agatcgtgct catccatcag gtcgagaatg
     7081 cgccccaggt tgcgatcgca catcgacacc agcgaacgat accggcaacg gatgtgctcc
     7141 acggcctcgt cgctttcctg cacccggcca cgcggccagt cataatgcgg tccatcccag
     7201 ttatcttcat acaaggaaag atactcttcc gttgtgtgaa acggctcgtg cggatcaaac
     7261 gtttcgatct gcaataacca gttatcttcc gcgtgatttt tgtggataaa ggcacagcct
     7321 gcatcaaaca ctttagtttg tggaaaatcc gcttcctgct gaatatattc ccggtttgcc
     7381 cagtcatggc gccacagacc agaaacaccg ccaccggtct gcttttgcgg cacccgtagc
     7441 acctcgggaa taggcggttg cgcaacgctt gcatgccagt gatcgccttc ctgtccgcgc
     7501 acaatctccc acgaactgta gcgattatga taattcccgc cgccatcttc ccagtaatgc
     7561 aaatgatcgc ttatcaggtg ggtatagatc cccgcctttt taagcagctc cggcatggaa
     7621 tcgtcaaacg gttctaacgg cccccactcc cgatgcagga agttacatcg cccggtatgc
     7681 aactcccgcc gggcaggcat acacggcata ctgccgacgt aactgttttc aaaggtggcg
     7741 gcatgagccg ccagacgttg gaaattaggc gctttggtta acgcatcgcc ataaggtggc
     7801 agataacgtt tattcagact atcgaacagc agaataatcg ctctcattac gccctacctt
     7861 attgattaat taaatgcgga aaatgttgct gaaccacttc ataaatttgc tgaccaatag
     7921 tttcaccgtc cataatattc tctatcggaa tcaaatattg cccttgtttc gggtcaagac
     7981 taaccacatc gagtaatttt ttggaaatag caaaaatatc cgcccagtcg gtaaatccgc
     8041 cgacttcacc tgcggcggta tgttccattt caactttgaa atcatatttg gtcagcgctt
     8101 ttttaacgac cgtttgtaac ataacgctac tgcctacacc gtaaccacag acaaacagaa
     8161 tattaattgt atctttcatt tttaaaccct taatatttaa ttaacccgcg taaggaccaa
     8221 gatggaatgt tgcagcgata aatttcagaa cttcacaaat agcaggccat acggttgccc
     8281 agtcaaatat tcctgtccag ccgacgccat tttcagcaag cccggttaac ggaatagccc
     8341 acaccgtacc gaaggtctga ataataccca gcaagaacgt acagataatt acggaacggt
     8401 atccgcccat gcggtttgct aatacgccaa ttggaccgcc agagaaaaac agaggtacaa
     8461 agccaggcaa taccataatt gggctgtgaa ccagtaataa gatccccatc gaaaaaatgg
     8521 tgccaaaagt acagaagacg aagcctaacg ttgcggcatt gggtgaaaaa ggtaacagcg
     8581 ctgcaacatc gacagcagga atagcgttag gaataaagcg atcttgccag cctttaaacg
     8641 aaccgttaat ttcgcccacc atcatgcgca caccctgcag cagaatgacc atatacatgg
     8701 agaactgtaa gccaaggttg atgatataga tgtaccaggg cttgcctttc gccattaact
     8761 ggacattatc gatacccgtt gatagcaaga aaccaccgac aaacagcgtc atcacaatgg
     8821 cgatagccgt tacgttatgg ttaaaaatcg ccagccaccc gggaagtttc aggttttcag
     8881 catcgtgctt ttcagggtca ccaaatttat gggcgaattt ggagaaaaac cacaatccca
     8941 acatctggtt atgagcaatg gtaaaaccgg catctttggt aactttggca atgggcttaa
     9001 caatgagcgt ggtggagaac gcccagaata cacccgttaa tattccggca ataataatgg
     9061 atgtagtcca gctaaaaccc agccaaaaga caatcaacca taataccgcc tgagaatgtg
     9121 aaacaccggt attccctgtc aaaaatatcc ctttggcacc agtataccga ccaaataata
     9181 ccagcagcag gttggtaaaa aacgccagta caaatacata accgacataa ccaaaattat
     9241 cccccaacgc aatttgcgtt gccgctttca ttgcgtagga atccattaaa tatccgtgaa
     9301 caccataggc tttacttacg gcttctgcta ccggtttaaa tgttgcggaa aactggaaac
     9361 caccaaataa caccatcatt aatcccacca tggcgctgac catgccggta ataattttta
     9421 ttggcgattt cttatcaagg gcataaccaa tacccaccag caaaccaatt aaaattgccg
     9481 gttcagttaa aaacgtttga ataacatcta tgaatttggc aaacatcgtc ggaacgccct
     9541 ccataagcgt acatttccgt tgtcaatgca tgaaccttct gctgacgtta ataatgtctc
     9601 aggaaatttt gtgtttcgct aagcaatgcc tctgtatgta ttcggtaagt gcactgtagt
     9661 aatcaaaaca gttcaattaa agatcttatt tgattatttg tgatttgatt tgcaaaaata
     9721 gagcaatata taaccgtgat cgatcggtta ttattaatcg atcgatcacg attattattt
     9781 tggatggatg tatgccgagg tgatattgat ttgaatgatg tatttacatt tttaccagca
     9841 gcgtcagcac ttcatagtgc gcggtgtgcg ggaacatatc gaaaagctgc acccgttcaa
     9901 tgcgataacc aggcagttcg cggatatctt tcgccatggt ttgggcgtta cagctggagt
     9961 agatgataaa acgcggtgcc atcgttgaga gataatcaca cagcggttta ccaatgccgc
    10021 ggcgcggcgg gttaaccagc accagctccg gcacctcccc ctgagcggtg gcaaactgag
    10081 tggagtccag cgcctgaaat tgcaaacgcg ttaagcccag ttcagcagct gactgctttg
    10141 cacaggcaat ggcctctggc gcaatttcga tcccggttaa ctgcatgtca ggcgtcgcgc
    10201 agtgtaaacc aaagcccccc acgccgcaga acagatccca catatgttta accggcagct
    10261 gtcgtaccca gtcgcgcgcg gtagcgtaca gctggctggc gaccgccgga ttagtctgga
    10321 agaaactttg cggacggatc cacagcggca catcgttaaa acgctctgcc agcgcctgtt
    10381 gttcggtcag gtagatctcc gtctcccctt ccataatcgc catatgtacc ggctgaatat
    10441 tgacggtgat aacttttagc tgcggcaatt gtgcctgtaa ccacggcaat gccttacgca
    10501 gttgtgccag tttggtttca gaacgcagta caaagcgcag catcatgccg ccatcgctct
    10561 ggctttcagt cagcagaatg tatttcagtt cgccacgctt acgcgccacg ttgtagggcg
    10621 ttaaccccgc tcgggcgata aacggtttta gcgccgcaaa aacgggcgca aatgaggcag
    10681 gataaagcgg gcagtcacaa aggtcttccg gtgtgccgtc gcgatgcagc ataccgagca
    10741 gtggtttttc aacgctacca ctcaccacca ttttggcttt attacgaaac ccttgttccg
    10801 ggcctgacac cggcgcgcac cattcctcaa ccggaaagtc ggcgagcaga tttttaagat
    10861 cggcggtttt agcggagagt tgctctggaa tcggctgcgt tatccactga caggaacgac
    10921 agcgacccgc gtcgtaaagt gcgcactgca tacattgacc ttcacatcat caggggcgac
    10981 gattatacac attattgcaa ctggaagaac cgccgactgg tcgagggaac gaacagcagc
    11041 atcaggatga gcatatccgg cagcttctgc agcatcaggc tatggaagat ttcacgtttt
    11101 gattcaccgg gaatgctgaa cagctccgga taaccgtacc ccagcgaagc cgcccacaag
    11161 taacttgcgg ccgtgatttg tgtcagcaga tatagccatc gcgcccagcg acgccctttc
    11221 accagtgaaa acgcacacca gatctcaatg aaaaccagca ccagactgct taaaaagacc
    11281 agcgttaaac tccaggtttg tacgctgcga tgaatgaact cgccaatacc gcgcacgccc
    11341 agcgtattga aaatcatcag cacgtcgagg ccacggatca taataatggc gagcgccgcc
    11401 acctgcacca gcgcaggcac attcaggcga gcatgagatg aagatgtttt cttaaaaaat
    11461 cccaacgttt cgtcttccat gaaaacaatg ccgcgacata cgcggcatta tgtagccagg
    11521 ttggcaaatt ttagtgtctt cagccacgtc ttgcacgctg gatgtcgcgt atccgctgtt
    11581 tttctgcgcg agccatcaga taccaggcga taaatccgac aattccgacc gcgccgagga
    11641 tcaatgttgc cagggcgttg atttccggat tcacccccat ccgcacgctg gaaaagacca
    11701 gcatcggtaa cgtggtggct cccggcccgg aaacaaagct ggcgatcacc agatcatcaa
    11761 gcgacaaagt aaaagccagt aaccagccag aaatgatcgc gggcatgatc atcggtagcg
    11821 taatgacgaa aaacactttc agcggcgtcg caccgagatc catcgctgct tcttctatcg
    11881 agcgatccag ttcccgcaga cgcgacgaaa taacgactgc cacataagcc gtacagaaag
    11941 tgacatgcgc cagccagatg gtaagcatac cgcgttccgc aggccagcca atggcatgag
    12001 caagcgcgac gaataataac aacagcgaca agcccgtgat gacatctggc attaccagcg
    12061 gcgcggtgat cataaaggca aagccatttg atccgcgaaa cctgccaaag cgcaccagca
    12121 ccaccgccgc aatagttccg aggatcgccg ccgccgtcgc cgcacaggcc gcaattgtca
    12181 gacttaaacc aaccgcactc atcatcgcgt catcgcgcaa taattcgcca taccagcgcg
    12241 ttgaccagcc ggcccacacc gtcaccagct tcgagctgtt aaacgaatag ataaccagca
    12301 tcagcattgg cgcgtagaga aaggtgaagc ccagcagcaa aatcacaatc cgccagggcg
    12361 aacgaactac cggtaaatta ttcatccgtg ttctcccacg cttttttgct ggtgtttgtg
    12421 gaaccacatt atcggcacaa ttagcagcag caacatgatg atcgctaccg ccgaggccac
    12481 cggccagtcg cggttattga aaaactcttg ccatagcacg cgcccgatca tgatgctgtc
    12541 cgggccaccg agcagttccg ggatcacaaa ctcgcccaca gccgggataa acaccagcat
    12601 cgatccggca ataatcccac ctttagtcag cggcacgatc acagtaaaga acgttttcag
    12661 cggtcgtgca ccgagatcca gcgctgcttc caccagcgaa taatcaatac gaatcaacgc
    12721 ggtataaatc ggcagcacca taaacggcac gtaggcgtaa acaatgccaa tataaactgc
    12781 cagattggta tgaagaatgg tcagcggttg atcgataacc cccagccaca gcagaaaatt
    12841 attcagcaca ccgttgtttt ttaatattcc catccaggcg tagacacgaa tcagaaacga
    12901 ggtccacgac ggcaggatca ccagtaataa taagatatta cgggtcgaag gcttactgtg
    12961 cgccaccgcc cacgccagcg gatagccgat cagtaaacag caaatagtcg aaatcgccgc
    13021 cacctgtaac gactggagat aagcatcgaa atagagcgga tcgtcggtca gttgcagaaa
    13081 attaccgaga ttaagagtga tggaaaattg cccgtcagcc cactccatca gttcggtata
    13141 aggtggaata gcgcgcgcca tctctgccag gcttatttta aagacaatca gaaatggcag
    13201 cagaaacagc aagatcaacc agatatacgg caacgcaatg accagtttgc gcccatggtt
    13261 catttgcaac tgcgacaacc acagcttaaa tccgcccggc ggtttcgact gggcagcagg
    13321 ttcaagtgta ctcatctttg ctccttaaac cgtaagcacc acacagctgt ccacttccca
    13381 gcacaaacgc acttcgtcgc cccaggtcgg taacccttta cgatgacgat gggcgttttg
    13441 tagctgggcg ctaatcatct gcccactttt cagacgaacg tgatacaccg aaagatcgcc
    13501 gagataggca atgtgtatca cctcccccac cgcgaagtta caaccattgg cgggcggctc
    13561 ttcgcaaagc atgatttttt ccggacgcag cgctacgtgt accggcacgt tatcgaccac
    13621 tgaagcatcc gcatcgactt tcagtggatg caccagcccc ggcgaataaa gcaccagacc
    13681 atcttcctga cgctctttga gtacaccttc aaagacatta accgagccaa taaattcggc
    13741 gctatagcgg gtagtcggat gctcgtagat ctcttccggt tcgccaatct ggacaaattt
    13801 cccacgattc ataatggcga tacgccccgc catggtcatc gcctcttcct gatcgtgagt
    13861 aaccatcacg caggtcacac cgacgcgctc cagaatatcc accacttcaa gctgcatccg
    13921 gtcacgcagc tttttatcca gcgcgcccat cggctcatcg agcagtaata gtttcgggcg
    13981 tttggcaagg cttcgggcca gggccacacg ctgtcgctga ccaccggaaa gctgatgcgg
    14041 tttgcgtttg gcgaactcct gcatatgcac aaggccgagc atctcattga cccggctggc
    14101 aatttccgct ttcggtagtt tgtcctgttt caggccaaaa gcgatgttct gttccacggt
    14161 catatgcgga aacagcgcgt aagactgaaa catcatattg atggggcgca ggtaaggcgg
    14221 cacctgtgac aaatcgacgc catcgagcat aatctgcccg gctgaaggtt gttcgaaacc
    14281 tgccagcata cgcagcagcg tggacttgcc acagccggat gcgcccagca gcgcgaagat
    14341 ttcacctttg tagatggtca ggctgacatc atccaccgca tgttgaccat cgtaggattt
    14401 ggtcaggttg cggatttcta atagcggcgt cagcgcctta cgggttttcg cctgcgggcg
    14461 agggatagcg tcattcacgg ctgctctccg gcattgaatg tgtgcaagcg caccataatg
    14521 gtgcgaattg ccggcgggtg tggtgcgccc gtccggcatc taaggctgcg gattattttc
    14581 cgctcttcac tttggtccac gcgcgggtgc gcacacggtc gattttcgga tcctgtactt
    14641 tcagagtgaa caacttcgcg cggacatccg caggcggata aatacccggg ttatcacgga
    14701 cttccgcact caccagcggc gtggctgctt tattggcgtt agcatagaac acatggtcag
    14761 agatatgtgc caccacatca ggacgcagca ggtaattcag gaactgatag gcttcgtctt
    14821 tatttttggc atccgcaggc atggcgaata catcaaagaa cgccatcgcc ccttcttttg
    14881 gaatcgagaa cgagacattc acgccattct tcgcttcctt cgcgcggttt gacgcctgcc
    14941 agacatcacc tgcccagccg atagcgacgc aaatatcgcc gtttgccagg tcgttaatgt
    15001 attgagatga atggaaataa cgaatgttcg ggcgcagctt taacagcaga tcggtagccg
    15061 gtccggtgta atcatccgct ttagtgctgt tgggatcttt gccgagataa ttcagcacgg
    15121 tagcaaaaac ttcttctggc gcatcgagga aagatacacc gcagcttttc agtttttcca
    15181 gattttcagg cttgaggatc aagtcccagc tatcgacggg cgcgttttcg cccagcaccg
    15241 ctttgacttt atcgacgtta tagccaatcc cggtcgtcgc ccacatatag ggcatagcaa
    15301 atttattgtc gggatcgtgt ttggcgacca acttcagcag ttccgggtcg agattcttcc
    15361 actccggcaa tttgctttta tccagcggct ggaaaactcc cgctgtcagc tggcgttcaa
    15421 gaaagcttgc tgacggcacc accaggtcaa agccggtgct cccggccatt aatttgcctt
    15481 ccagtacttc gttagagtcg aaaacatcgt agacgacttt aataccggtt tctttttcaa
    15541 agttagccac cgtgtcgggg gcgatataat cagaccagtt ataaatgtgg agtgtttttt
    15601 gttcagccgc gagcgtgccg acagagacgg ccatcagagc tcccgcaacc agacccgaaa
    15661 gccatttttt atttaaggcg gtcatatctc tttccttctg aaagttcgtt aacaaacgaa
    15721 caaaagttgt cacactgaaa gatatgcaat aaacgtgcat attttttatc ggcgcggtgt
    15781 aagaaaagag aactctctcc cggccagcac tgctcgctat ataaaagcat cgcaagaata
    15841 actttagcca gggtttgaga ctatagctcg gattaaaaaa cggtatttaa caaattttta
    15901 tgcgtatttg ctctatgtga taaagccaaa tcgacacaag agaagtgaaa taatctttaa
    15961 gaaaaggtta aaaacagcag ataattttgc agcgcgcagc cactgcacca gcgactgcgt
    16021 gctcgaaagg agttatcagt ggaggaaata gttttcggta acgttgtttt gcccttcatc
    16081 gtcagtcggt aacagcagtt gatgggcatt agcttcaaga atgaccatcg aaatctgctc
    16141 ttcgctctga cgcacgaacc atgcaaactg ctcataggtt acgccctgca taacggataa
    16201 agactggcaa accaccagct ttggcagatt atcatcctgc atatcaagaa atgctttcac
    16261 ggtcagcgaa ctggcattga tggcagataa atccgccgcc agcggcaata cggctgaagg
    16321 tcggacttcc gccatggcag aaaacaggat cgtgttatcg atcagatcga ttttggcatc
    16381 aaagacaccg tcgaaattct gcatatgggg cagatgcaga gcctgacagt tatcacattc
    16441 aaaaaaactc atccccaggt catcgagcca ttgacgcagc gtatccagac caggaacgac
    16501 cagcgatgtc ataattttgt acaacctctt tcgataaaaa gaccggcaca gcttacgcaa
    16561 aaagcgcagg caaaaccatg atcagtaatg tgattgcgac taaccacccg ttttcaggca
    16621 atattctgtc gtagcgtggc gttcgatcca gcggatcatt ttacccgcga tgtcgatacc
    16681 ggtggttttt tctattcctt ccagccccgg cgacgcattc acttccatca ccaacggccc
    16741 gcgattagca cgcagaatat ccacaccagc aacgtccagc gccatcgttc gcgcggcttt
    16801 tatcgcgatt tcacgctcct gtggtgtgat actggcgacg cttgccgcgc cgccacgatg
    16861 caaattggaa cgaaaatcgc cctctttcgc ccgccgttca atcgcagcga ccacttcatc
    16921 gccaacaacc agacagcgga tatcgcaccc ttgcgcctct ttgatatatt cctgcaccag
    16981 aatatgcgcg ttcagaccgc ggaaagcgtc aatcacgctt tccgccgcct gacgtgtctc
    17041 cgccagcacg acgccaattc cctgcgtgcc ttcaaccaac ttgaccacca gaggcgcacc
    17101 accgaccatg tcgattaaat cgctggtatc atccggcgaa tgcgcaatgc ccgtgacagg
    17161 caggtcgatg ccctgacgcg ccagcagttg catggaacgc aatttgtcac gcgcccgggc
    17221 aatggcgacc gactcattga gcggatagct ccccagcatc tcgaactggc gcagtgccgc
    17281 cgtcccataa aaggtaatgg cggtgccaat acgcgggatc actgcgtcaa aatggggtaa
    17341 cttgcggcct ttgtagtgaa tagaagacgc cgcaggattt atgttcatgt agcaagaaag
    17401 cggatcaaga atttcaacca ggtgaccgcg ctgtatagcg gcttcacgca gccgcttaca
    17461 cgaatagagc gttccatccc gggacaatat ggcaattttc accctgcacc tctctgtcag
    17521 acctggtcaa aagcccgcgt atcatacatc gacgtggcgg ttttagcgcg tcgcccaacc
    17581 ctgtttgtgc aaataatcca gaataaatgg gcggctttct ttaatgattg ttcggcggat
    17641 atgatcgctc caggtatccc ggcgattatt gctgccacgg gtgaggtaat attccgccag
    17701 ttgctcgtca tactgcgcca gtgcgtcttt atccagcggt tgatagctgt tttcatgcac
    17761 caaaatggag gccggtaaac gcggcttgag atccggatta tccgcaggcc agccaaggca
    17821 cagcccaaac agcggcagaa catgctgcgg taatttcagc agtttcgtca ccgcttcaat
    17881 attattgcgc aggccgccga tatatacgcc gcccaatccc agcgattccg ctgcggttaa
    17941 tgcattctgc gccatcattg ccgtatcaac gacaccgagc aacagctgtt ccgccaggcc
    18001 gagctgagca tccggacaga tctgtaaatg gcggttaaag tcggcacaga acacccagaa
    18061 ctccgccgct tgcgctacgt gtttttgccc gccggtcagc gtcaccagct cttcacgtaa
    18121 tgctttgtcg gtaatgcgaa taatgctact acactgcaaa aaactggaac tggacgtcgc
    18181 acgggcgctg ttaataatcg cctcacgctg cgcttcggaa atgggttcat cagtgaaatg
    18241 gcgaatggag cgatggccac aaataagttc aatggttggc gtcattatct ttttctcttt
    18301 ctgaacgtga atattgcggt ggacggttca tcagctgtgg ggcaagacgt tttgccacct
    18361 gaagaataac caccaccgca gcgggaagca tgagcaaaac accgagaaaa atcatcagaa
    18421 tctgcacttc tggccgagaa aatggctcag gcagcgacag ggagtcgctt accgacagca
    18481 gcgccaccgc cagcaacatc attccgataa attccagtat caacacgctt ttaggcaatt
    18541 taccgatcgc gcgcatacgc ttccctctgc aaagtgagcc ttcagtctaa aacttttcac
    18601 tgtattgtgt ttaacagtta tagcttttag caattaatgc aacaggttaa acctactttc
    18661 agcgaataca ttttagcgtg atcattacag gcataaatct atgaggagag aaataatgca
    18721 aaccgttatt tttggtcgcc cgggttgccc ttactgtgtg cgtgcaaaag atctggctga
    18781 gaaattgagc aatgaacgcg atgattttca gtatcagtat gtagatattc gtgcggaagg
    18841 gatcactaaa gaagatctac aacaaaaggc aggtaaaccc gtagaaaccg tgccgcagat
    18901 ttttgtcgat cagcaacata tcggcggcta taccgatttt gctgcatggg tgaaagagaa
    18961 tctggacgcc tgatcgtctg acaagccctc gcgttgaggg ctttactgat tttttctgtg
    19021 ctgtggttta aacaagctac tgataaataa gaaacacagt gcccccagcg cacaccagaa
    19081 caccgcgctt agtaaccatg ccagctcttg ccagaatgag cgggtcggtg aaaaaaacag
    19141 ccgcataata agcattgaac agggtgccgc cagcattgcg ccaaatagag gtttcaggac
    19201 ttctctacgc tgtgaaaaga aactggcaac tgctccagga agaatgaaaa acagcaagcc
    19261 gatttcagga tgcccggcag cccgaaaagc gcctttcatg tgcgtcgcca gaaaaaggca
    19321 caccacaatg aagaggacaa aacagcagat tgcccccgcc caacgttgtt tatgtttcac
    19381 tcgttcctcc tgacactgcg tctatcgaac acatttttcg ccagtgtggc gttcagtaag
    19441 ataaagccgc ttcgcattcc atgctaatat aggccaacgc aattcatata gccgttgata
    19501 cctaatgtga ttacactagt aaaatatatt gttactttac tatcgtttag gtgcgctgaa
    19561 tgaatctgcg ccctgaattc tggtaaaaaa cattatcgta aattaccatt tctttcaaca
    19621 gcttactagt aaacaagaag ttagcctccg tgaatataaa cgtcgccgaa ttgttaaatg
    19681 ggaattacat tctgttatta tttgtggtcc tcgcgcttgg gctatgtctc ggaaagttac
    19741 gacttggttc gatccaactg ggtaattcca ttggcgtttt agtcgtatcg ctgttattag
    19801 gccaacaaca tttcagcatt aacaccgatg cgcttaatct tggctttatg ctgtttattt
    19861 tctgcgtcgg ggtcgaagcc ggaccgaact ttttttccat tttttttcgc gatgggaaaa
    19921 attacctaat gttagcactg gtgatggttg gcagtgcgct ggtgatcgcc ttagggttag
    19981 gtaagctgtt tggctgggat attggcctga cggccggtat gttagcaggc tctatgacgt
    20041 cgacaccggt tctggtcggt gctggcgata cactgcgtca ttccggcatg gaaagcaggc
    20101 agctctcact ggcactggat aatctgagcc tcgggtatgc cttaacctat ttaatcggtc
    20161 tggtgagttt gattgttggt gcgcgttact tgccgaaatt gcagcatcag gacttacaga
    20221 ccagcgccca gcaaatcgcc cgcgaacgtg gcctggacac tgatgccaac cgtaaggttt
    20281 atttaccggt gatccgcgcc tatcgcgtcg gcccggagct ggtggcctgg accgacggca
    20341 aaaatctgcg tgaactgggt atttatcgac aaaccggctg ctacattgaa cgtattcgac
    20401 gtaacgggat tctggcaaat ccagacggtg atgccgtgct acaaatgggc gatgaaatag
    20461 cgttggtagg ctatcccgac gcccatgccc gactcgatcc cagcttccgt aacggcaaag
    20521 aagttttcga tcgtgacctt ctcgacatgc gtatcgtcac tgaagaagtg gtcgttaaaa
    20581 accataacgc cgttggcaaa cgtctcgcac aactgaagtt gaccgatcac ggttgcttcc
    20641 ttaaccgcgt tattcgtagc cagattgaga tgccgataga tgacaacgtc gtgcttaaca
    20701 aaggtgacgt tttacaagtc agcggcgatg cccgccgcgt aaaaaccatc gccgatcgca
    20761 tcggctttat ctcgattcac agccaggtca ctgacttgct ggcattctgc gccttctttg
    20821 ttattgggct gatgatcggg atgatcacat tccagttcag cacattcagt ttcggcatgg
    20881 ggaacgctgc cgggttgtta ttcgccggaa ttatgctggg ctttatgcgt gctaaccacc
    20941 cgaccttcgg ttacattccg cagggtgcat taagcatggt gaaagagttc ggcttaatgg
    21001 tgtttatggc aggcgttggt ctgagcgccg gtagcggtat taataacggt ctgggcgcga
    21061 ttggcggtca gatgttgatt gccggattga ttgtcagtct tgtgcccgtg gttatctgtt
    21121 tcttgttcgg tgcttatgta ttgcgaatga accgcgcact gttgttcggc gcaatgatgg
    21181 gcgcacgcac ctgcgcgccg gcaatggaga tcatcagtga tacagctcgc agtaacatcc
    21241 cggcgctggg ctatgcgggc acctacgcaa tcgccaacgt cctgctgacg ctggcaggga
    21301 caatcatcgt catggtatgg ccaggattag gataaaactg aagttgccct gaaaatgaaa
    21361 tttttttgca caaccgcaga acttttccgc agggcatcag tcttaattag tgccactgct
    21421 tttctttgat gtccccattt tgtggagccc atcaaccccg ccatttcggt tcaaggttgg
    21481 tgggtttttt gttgcctaaa atttatctac tacccagcaa ccctctcaac catcctcaaa
    21541 atctcctcgc gcgatagcgg cttacggtcg gtgacaaaat gcagcgtcat cccctcaata
    21601 aacgcatcaa gcgcgcgggc ggttccgggt tcaaaccatt gttcgagcgt ttgctgactg
    21661 cgctgcatcc agttttgcat taccgttttt aatagcggtt ttcggctagc cagcgcgtag
    21721 agctggtaca tcagctccat gttatccggc gttgccacct gtgagctgta gatcatatcg
    21781 gtgatagcct ggcatgcgcc ttgagcatca ataacatcgc taaaaaatgc ctgatattgc
    21841 cgggacatga tctcagtaaa actgctgaac gcctccagca acaactcatc aattcctgaa
    21901 aaatagtagg tcatcgatcc caacggtacc ccggcaaggg tagcaatttt gcggtgcgta
    21961 acagcatgta ttccgtaaag tttcaccgcc tccagcgtgg cctggataat tttttctcgc
    22021 cgttgcggat cgttagcgcg acgcatgtca tttcctctat ctgcaattgt gtacaaatgt
    22081 acacaacctt gctaatgttg tcttccctct tcttattttg acgtgatcta tgaccgtaaa
    22141 ttcttcacgt aatgcattga aacgccgaac ctgggcgctg tttatgttct tctttttgcc
    22201 gggcctgtta atggcgtcct gggcaacccg tacgcctgct atccgcgata ttctctctgt
    22261 ctcgatcgct gaaatgggcg gtgttctctt tggtctgtcg atcggttcga tgagcggtat
    22321 tctctgctcg gcgtggttag tgaaacgctt tgggacacgt aatgtcatcc tggtcaccat
    22381 gtcctgcgca ttggtcggga tgatgatatt aagtctggca ctctggctga catcgcccct
    22441 gctctttgcc gttggtctcg gcgtctttgg ggcaagtttt ggttctgcgg aagtggcgat
    22501 aaacgttgaa ggtgccgccg ttgagcgaga aatgaataaa acggttttgc cgatgatgca
    22561 cggtttttat agcctgggca cgctggcagg cgctggtgtc gggatggcac tgacggcctt
    22621 tggcgttccg gcaacggtgc acattttatt ggcggcgctg gtaggcatcg cgcctatata
    22681 tatcgccatt caggcaatcc ctgacggtac gggcaaaaat gctgccgatg gcacccagca
    22741 tggcgaaaaa ggcgtacctt tttatcgcga tatccagttg ctgctgattg gtgttgtggt
    22801 gctggcgatg gcctttgccg aaggttctgc caacgactgg ttacccttat taatggttga
    22861 cggtcacggt tttagcccca cttccggctc gctgatttat gccggtttta ccctggggat
    22921 gaccgttgga cgcttcactg gcggttggtt catcgaccgt tacagtcgcg ttgccgtggt
    22981 tcgggccagt gcgctaatgg gggcgttggg tattgggctg attatttttg tcgatagcgc
    23041 ctgggtcgct ggggtgtctg ttgtactctg gggactgggt gcctcgctgg gcttcccgct
    23101 gaccatttct gccgccagcg ataccggccc cgatgcacca acccgcgtca gtgtggtagc
    23161 aacgaccggt tatctggctt tcctcgtcgg gccgccgctg ctgggctatc tcggcgaaca
    23221 ttatggatta cgtagtgcaa tgctggttgt actggcgctg gttattctcg cggctattgt
    23281 cgcgaaagcc gtcgccaaac ccgataccaa aacgcagacg gcgatggaga atagttgatg
    23341 agcattaaat taattgcggt agacatggat ggtactttct taagcgatca aaaaacctat
    23401 aaccgtgagc ggtttatggc tcagtatcag caaatgaaag cacaagacat tcgctttgtg
    23461 gtcgccagcg ggaatcaata ttatcagttg atctctttct tccctgaaat tgctaatgaa
    23521 attgcctttg tggctgaaaa cggcggctgg gtagtgagtg aaggcaaaga tgtttttaat
    23581 ggcgagctgt cgaaggatgc gtttactact gtcgtggaac atttgctgac gcgcccggaa
    23641 gtggaaatta ttgcctgtgg aaaaaatagc gcctatacgc tcaaaaaata tgacgatgcc
    23701 atgaaaacgg tggcggaaat gtattatcac cgtctggaat acgtcgataa ctttgacaac
    23761 ttagaagata tcttctttaa gtttggcctg aatctttccg atgaactgat tccacaagta
    23821 caaaaagcat tacatgaggc catcggcgat attatggtgc cggtccacac cggcaacggc
    23881 agcatcgatc tgattatccc cggcgtacat aaagccaatg gccttcgcca actgcagaaa
    23941 ttatggggaa tagacgacag cgaagtggtg gtctttggcg atggcggtaa cgatattgag
    24001 atgctgcgtc aggcaggctt tagttttgca atggaaaatg ccggcaacgc ggtcgtcgca
    24061 gcggcaaaat accgggcagg ctccaataac cgtgaaggcg tactggatgt gatcgataaa
    24121 gttcttaaac acgaagcgcc atttgaccaa taaaaacggc agcacgcccg gcaacacaat
    24181 acctgaatga aggctgcata attcgcggaa attaacccgc tgcattattt caggcgaaat
    24241 gtttatgaaa aattgtctac tgttgggcgc gcttttaatg ggctttactg gtgtggcgat
    24301 ggcgcaaagt gtcaccgtcg atgtgccgag tggatacaaa gtggttgtgg ttcctgattc
    24361 agtcagcgtt ccacaagcgg tcagcgtcgc gactgtgccg caaaccgtct atgtggctcc
    24421 tgcgccagct ccggcttacc gcccgcatcc ttatgttcgc catcttgcca gcgtaggtga
    24481 agggatggtt atcgaacacc agattgacga ccatcaccat taattcgaga atgcctgatc
    24541 gcacaaagca atcaggcatt tttttaccct tcgtgagaat ttcccatctg tttatctttt
    24601 aaaaagataa ccatcagcga cagccacaaa attccgttga caaggttgaa gagattaaac
    24661 agtccgttgc ccccgttcag ccaggcatgt ttgctgattt caataccgac ggtaaagatc
    24721 agcatttgca gcattcccat cgccgcagaa accgtacctt tactcatatc gctggcaaac
    24781 agggttaatc gcaccagtcc cgcattcgcc agaccaatac cgaaagcata aatacttaac
    24841 ccggcggtca tccataaata cgcgtgcgat gagataaccg ttgccgcagc agcgaccaac
    24901 agaccaatca taatcggcca gccgcccata ataatcagcg aacgtacggt gcggcgcgag
    24961 gtcagacgcg ctaacagcaa gttacccgca attaacgccc cgaaaatagg cacttgcagc
    25021 aaaccatatt catagctgct caactgctcg ccggtaatga tgataatcgg cgactgggcg
    25081 atccacgcga gcaacggcag gctaacgaat cccagcgcca gcgcccccgc cacaaagcga
    25141 ccgttcttca gcaccagctt atagtcacga ccgagttctt tcagcgacag tttctcgcct
    25201 atacgcgtgg cggtttcagg catggctcgt tgcagaccga aaaaggagat cgctgccaat
    25261 gcggcaaaca agacaaacat cccttcccag ggcagcacat ggatccacgc cgcgcccacc
    25321 agtggaccaa gtagcggagc aatcagcgcc acgttcgcca tcagcgcggt gatcttgata
    25381 caaaccgcct cttcgaagga ttcctgaatt gcggcgtatc ccacagcgcc aatgaaacag
    25441 aggcttatgc cctgcaagaa gcgcaacagg gtgaattgtt caatgttttg cgccagtaat
    25501 attgccagac aggtgacaat aaaccacacc acacccgcca gcatcaccgg acggcgacca
    25561 atacgatccg acaacggccc caggagccat tgtaaaaaca tcccgcccgc cagatatgcg
    25621 gtcatcgaag taggaaccca atcaatgccc gcctgatatt gttccaccac ggccaacata
    25681 ccgggttgaa tcatatcgtt gccgatatag gttgaaaatt cgtacagcac cagacagaga
    25741 gggaaaagta acgcctgacg tccaagcctg gcaccggaag ctaatttatt ttgcatgcaa
    25801 tttcttcgcc aataataatc gcgcaaagtt taataaaagc gcagctaacg agaaagcgaa
    25861 ttttgtagct gaaaccgcgg ttaagcacat tcttacatta ttgcgagtat agctacgctt
    25921 tctttaagtt ttatttaacc tatgcccgtt acaatcaccc accgtaaaca ggccgcttga
    25981 gggaaataag acgatgccgc tttacccagt ttaacctgca ctttattctc aacgagttgc
    26041 ctgtattggc tcccttttaa tcactttgcg tcgggaagtt atgctggaaa atttgaatct
    26101 ctctctattc tctcttatta acgcgacgcc agactcggct ccgtggatga tctcgttggc
    26161 gatttttatt gctaaagatt tgattaccgt ggtgccgctg ctggccgcgg tactttggtt
    26221 gtgggggctt acagcacaac ggcaactggt gataaaaatc gctatcgcgc tggcggtcag
    26281 cctgtttgtg tcctggacga tgggacatct ttttccgcac gaccgaccct ttgtcgaaaa
    26341 tatcggctat aacttcctgc atcatgcggc ggatgactca ttcccgagcg atcacggtac
    26401 ggtgattttc acctttgcac tggcattttt atgctggcat cgcctgtggt ccggctcact
    26461 tttaatggtg ctggccgtcg tcattgcctg gtcgcgcgtt tatcttggcg tccactggcc
    26521 gctggatatg ctcggtggat tgctggcagg tatgattggc tgccttagtg cccagattat
    26581 ctggcaagcg acagggcata aactctatca acgtctgcaa tcgtggtatc gcttctgttt
    26641 tgcattaccg atccgcaaag gctgggtgcg tgactgatct ctgctaaaaa gtgtagtatt
    26701 gagcggctcg cttcaataac tattcagagg gattatggaa acacgtcgcg aagagcgtat
    26761 cgggcagctg ctgcaagaat taaaacgcag cgataagtta catcttaaag acgccgccgc
    26821 gctgcttggg gtttcggaga tgacgattcg tcgcgatctg aacaaccaca gtgcgcccgt
    26881 cgttttgctc ggcggctata ttgttctgga accgcgcagt gccagccatt acctgttaag
    26941 cgatcaaaaa tcccgcctgg tggaagaaaa acgccgggcg gcaaaactgg ctgcgacgct
    27001 ggtagaaccc gatcagaccc tcttttttga ctgtggcacc accacgccgt ggattattga
    27061 agcgattgat aatgaaatac ctttcaccgc cgtttgttat tcgctaaata cctttctggc
    27121 gctgaaagaa aaaccccatt gccgcgcatt tctgtgcggt ggtgaatttc acgccagcaa
    27181 cgccattttc aaacccatcg attttcagca aacgttgaat aatttttgcc cggatatcgc
    27241 tttttattcc gcggcgggcg tgcatgtcag taaaggcgct acctgtttta atcttgaaga
    27301 gttgccggta aaacactggg ccatgtcgat ggcgcaaaag catgtgctgg ttgtcgacca
    27361 cagtaaattt ggcaaggtgc gtccggcgcg catgggtgac ctgaaacgct ttgatattgt
    27421 ggtgagtgat tgttgtccgg aagatgagta tgtgaagtac gcgcagacgc accgtattaa
    27481 gttgatgtat taatgacgta taaccggatg acgtttcgca ccatccggtt atcagaagat
    27541 taagagaacc agctgccgaa ccactgatgg aatttcatca tcacgaaatc ccacatccga
    27601 ccaaagaatc cgccctcttc cacattttcc atcacgatca gcggacgctg ctcaatggat
    27661 ttaccgttaa gctggaaatc aatggtgccg acaacctgac cttttttcag cggtgcggta
    27721 agctgcggtt ccgttaacgt gtagctcgcc ttcaggtttt tcagctgccc acgcggtatg
    27781 gtcactgagc ccgcttcgcc tgccccgaga ttcacttcgc tcttatcacc aaaccagacg
    27841 cgctgagtca caaaggtggc atcaggttta attggcgtca cggtttcaaa gaagcggaaa
    27901 ccccaggtca ataatttctc agactcatta aaacggatac ggtcagtttt cgcccccagc
    27961 actacggaga ttaaacgcat atcgccctgg gtagccgaag caaccagatt atatcccgcg
    28021 cctgccgtgg ttcctgtctt catgccatca acattcagat tgctgctcca cagcagacgg
    28081 ttacggttag gctgacgaat tttgttgaag gtgaactctt tctctttatg aatggcgtac
    28141 tcttccggca catcgtggat caatgcttta cccagcaatg ccatatcgcg cgcggtactg
    28201 aactgccccg gcgcatccag accgtgcacc gtctggaagg tagtgttggt cagacccagt
    28261 tttttggcat aaccattcat caaaccaata aatgactcct ggctcccggc aacgtaatcg
    28321 gccagcgcaa tacaggcgtc attaccggac tggataatca cacctttgtt caagtctgcc
    28381 accgaaacct gatcgcccgg tttgaggaac attaccgatg aaccacgcag tgccggattt
    28441 cccgtcgccc aggcatcttt accgaccgtc accatatcgg tgagtttaat cttatcggcc
    28501 ttaagcgcct gcccaaccac atagctggtc atgattttag tcaggctcgc gggatccagt
    28561 ttctcatccg cattgccttc tgccagcact ttaccgctgg cgtaatccat taaaatccat
    28621 gcacgcgcat ccacgctcgg cgcttcaacg gtttgttcgg ccgcgaatgc cgttggggca
    28681 aaaaggaata aaaatgcaga acccgctgca agaccacgaa ggagagagga gtattgcgtc
    28741 ataaatgcca cccgagtatc cattcaaaaa aaatacgtta tatcgccgta tgataagttc
    28801 ggtgattaat aacgcacaca caaacttaaa gaaaccgtaa gttggtaaag tttttaaagt
    28861 ttatgcaata tcctgccgct tcgctgcttc tggtgtcatt tttttgccag ctgttgctta
    28921 aaagttaact ttacttcacc atcggagtcc tgttcagcct gtggctgcct gtcggaaaag
    28981 gggtgagtat gattacgctg tggggtcgga ataattcaac caacgtaaaa aaagtattgc
    29041 tgacgctcga agaactggaa ctaccttatg agcaaattct cgcgggccgt gagtttggga
    29101 taaatcacga tgctgatttt ctggcgatga accctaacgg gctggtgccg ttgttgcgtg
    29161 acgacgaaag tgatctcatt ctttgggaat caaacgccat tgtccgctat ctggcggcac
    29221 agtacgggca aaaacgcctg tggatcgact caccggcacg tcgtgcggaa gcagaaaaat
    29281 ggatggactg ggcaaaccag acactcagta atgctcatcg cgggatcctg atgggattag
    29341 tcagaacacc accggaagag cgcgatcagg ccgccattga tgccagttgc aaagagtgcg
    29401 acaccctgtt tgccctgctc gatgcggaac tggcaaaagt aaaatggttc tctggcgacg
    29461 agtttggtgt gggcgatatc gctatcgcac cgttcatcta caatttgttt aatgttggcc
    29521 tgacctggac accgcgtcca aatctgcaac gctggtatca gcaactcact gaacgccccg
    29581 cagtgcgcaa agtggtgatg attcccgtta gctaattgcg tgggctaact ttaagtaatt
    29641 ccccactgga ctcgtcggtg agaacgtata aataaccgtc gggtccagtg cgaacatcac
    29701 gaattcgctg ccctctgtcc gttaaaatac ggccatcttc tgtcactttg tcgccgttga
    29761 cgctcatcac aatgacatct ttatctttca gcgcgccaat aaataatttt tgctgccact
    29821 gggggaattt atcgctgtta tagaaggcca tgccgctcac ggcgggcgaa tctttccagt
    29881 aaaaaacagg ttgctcggtc ccggcgacga tctccccttt cgcttccggt atcttaaagc
    29941 ctgaatagtt gattccccag gttgccagcg gccagccgta gtttttgcct ttttgcggga
    30001 tattaatttc atcaccaccg cgcgggccat gttcattcag ccacagtgca ttactccacg
    30061 gattcatcgc cattccttgc ggattacgaa tgccataaga ccagatctcg gcgcgcgcac
    30121 cggattcctt tataaaagga ttatcatccg ggatttcgcc ctggtcggtc agacgcacca
    30181 gtttgccctg taatttatcc agatcctgcg ccgtcgggcg ctgattgttt tcgcccagag
    30241 caataaaaag ataaccttta ccgtcgaata ccagccgccc gccaaaatgg ttgccggtag
    30301 acagttttgg catctggcga aagacggtgc gaaagtcggt cacttttgag agatcatcac
    30361 ttaagcggcc ataacccaca gcagttccgg ctttgccatc atcgccaact tcggaataac
    30421 ttaaccagat gcggcgagac tgagcaaaat caggcgctaa aaccacgtcc agcaggccgc
    30481 cctgcccgtg cgcccaaacg tccggaactc cggaaagcgg cgcagataat ccttttcctg
    30541 cttgccagtg acgcaactcg ccgccgcgca gagtgattaa cataccgtga ttatcgggta
    30601 aaaaggccag tgcccaggga tggtcgagtt tgtcttgcag tacttcgaca tttaccgttg
    30661 caggagccgc ccagagagcg gaagaaagac aaataagggg cacaaggaaa aaggattgtc
    30721 gatgcataac gcctccggat tgactctggc ttaaagcgta gtcagtggag gagataacaa
    30781 attcattttt acaaaaactt aaacatgaag ggggagacgc tttctccccc ttagttttca
    30841 ggccttctca agcatggcgt gcttctgcag gctctggata ctcagcgtta agctcatcag
    30901 acaattttca agcttatcgg cgttgacggt aataacagtc gggcaatcat ggtgcccact
    30961 catcaaacat actgcggctg tcgctaatgc ttcttcagca tgatgaagag cactccactc
    31021 ttcctgatcc agatgaagat tcaaccgcag cgatttatcg tgcagttcgc gattcagttt
    31081 aaaaaagtta tctcgtagat gattgctttc gctgacggac atgtatcctt ttgccttcct
    31141 cagttgcacg tatgcggcga ggtcaacgcg tgcgtcgatc aaccggttca gcagatcgat
    31201 tcgctgtctg tcaacgaaca tacttcgatc ctcctctttc cagccttgtt acaattaaat
    31261 agtatggcta ttttttgagc aaaatgctgt ccaggatcaa ttcttcactc cgaattaaca
    31321 aaatttacat ttccttcgcc tccgccagcg aatgagattg cgatacaaat tttatccaga
    31381 actctgtaga atccctgccc tgacctttcc tgtaactgaa tattttttaa gctatgagca
    31441 aagtaactcc ccagccgaaa atcggctttg tttcccttgg ctgtccgaaa aaccttgttg
    31501 attcagagcg tattctcacc gaactacgca ctgaaggtta tgacgtggta ccgagctatg
    31561 acgatgcgga catggtgatc gtcaacacct gcggctttat tgacagcgcg gtacaagaat
    31621 cactggaagc cattggtgaa gcgttgaatg aaaacggcaa ggtaattgtg accggttgtc
    31681 tgggggcaaa agaagatcag atccgcgaag tccacccgaa agtgctggaa atcaccgggc
    31741 ctcatagcta tgagcaggtt ctggagcacg ttcatcacta cgtgccaaaa ccgaaacaca
    31801 acccattcct gagcctggtg ccagaacaag gtgtcaaact gacgccgcgt cattatgcct
    31861 atctgaaaat ttctgaaggc tgtaatcacc gctgcacctt ctgcattatt ccgtctatgc
    31921 gcggcgacct ggtgagccgt ccgattggcg aagtgttaag tgaagcgaaa cgtctggtag
    31981 atgcgggcgt taaagagatt ctggtgatct cgcaggatac ttccgcctat ggcgttgatg
    32041 ttaaacatcg tactggcttc cacaacggcg agccggtaaa aaccagcatg gtcagcctgt
    32101 gcgaacagtt atcgaaactg gggatctgga cacgtctgca ctacgtttat ccttatccgc
    32161 atgtggacga cgtcatccca ctgatggcgg aaggcaaaat cctgccgtat ctggacattc
    32221 cgttgcagca cgccagcccg cgcattctca aactgatgaa gcgtccgggt tctgtagatc
    32281 gccaactggc gcgcatcaaa cagtggcgcg aaatctgccc ggaactgacc ctacgctcaa
    32341 cctttattgt cggcttccct ggcgagacgg aagaagattt ccagatgcta ctcgacttcc
    32401 tgaaagaagc acgtctggac cgcgttggct gctttaaata cagcccggtt gaaggtgcag
    32461 acgccaatgc cctgcctgac caggttccgg aagaagtgaa agaagaacgc tggaaccgtt
    32521 tcatgcagct gcagcagcag atttccgccg agcgcctgca agagaaagtg ggccgtgaaa
    32581 ttctggtgat tatcgacgaa gtggacgaag aaggcgcgat tggtcgcagc atggcagatg
    32641 caccggaaat cgacggcgcg gtttatctca atggtgaaac caacgttaag ccgggtgata
    32701 tcctgcgtgt gaaagtcgag cacgccgatg agtacgattt gtggggtagc cgggtttaat
    32761 taattcaggc cgggcaaaat catttgtccg gcctgcgaat aatgattact gccagctaat
    32821 ctgtgccctg ccgtttttct tactcttata cagccgctca tccgccagca ttttcaagcg
    32881 ttcgacggta gtacattcat ttgaatgcgc tacaccgata ctggtgctta aattaatgct
    32941 tcccacctgg aaatgaacga tttcttttag aatcgatagc aactcttcca gcgttccacc
    33001 cgtataaatc accgcaaatt cgtcaccgcc gaagcggtag actttacctt tatctaccgg
    33061 gatctgtgat ttaaggattt tcaccacttt catcaacact tcatcaccca gcaaatgacc
    33121 gaaggtgtcg ttaatgtttt tgaatttatc gacgtcaaac aggcacaaat aaccgccttt
    33181 ttccggcagc gcttttaaat cttcgtcaaa ggattttctg cccattgctt tggtcaggtc
    33241 gtccaggaac gccgagttat acatactgta gagttttttg acatcggcag ccgaaaagaa
    33301 aagcccgcca tccagtacgc gttcaaaatg gttattaata atggcttctt tctccacaat
    33361 accacgatta atcactctgc gtaccagata gaggcaactg gtacccgata tcactgccac
    33421 caatgcggca aagaaaataa tcaatgtaca aacccgcaaa taaacatctt ttttatacgc
    33481 aaatacgtac aatccggtcg gcaaataggc gaagtgctcc atcgcttccg aaacggtgaa
    33541 gatggcgttt ttagcattat attcatcacc ggtcaccggt ttaccgttct taaacagaga
    33601 gaacatcagg ttctcgctat ccatcgccag agattttgac agtaaagaaa aacgctccag
    33661 accaagcaaa aatatcagac tacgattgtg agttaccgga tagaggtaat agacgaaggt
    33721 tttaccgtca aaagagataa accctgtatt tttgacatgt cgatacttca caacctgcat
    33781 aatatctgca caataatttt tctcgaactc ctttacctga atacattcgt cactggaaat
    33841 cagataagga ccgacatcgt acaaataaga gggtgaaacc gagtcggcgt aagctgtgcg
    33901 taagccaacc aacagctgtt cctccgtggg atttagcgtg ttgataagat caattgccac
    33961 gctacgccga tattcagcaa tatttgccac cgcaaaagca gtatcaataa cggactgttt
    34021 caggctatac acccgttcct ttaccatttg agtgtagatc ccgcaggcaa ctgctgaaat
    34081 catgataaaa atcagcagac tgactgtaag tacgaactta ttgattctgg acatacgtaa
    34141 actactcttt tactaatttt ccacttttat cccaggcgga gaatggcatg ggtttctggt
    34201 acagataccc ctgagcctga acacatccta atttgattaa ttcgcgcgca acgtcttcgc
    34261 tctctacgcc ttccgcaatc accgggatcc ggttatagcg tgccaggtcg aaaataatgc
    34321 gcacaatttt tcccgcagta ccgaaaatat cgccggtaaa ttgcgagtcc acctttatgc
    34381 tgtctggatt aatttcataa aaacgtttca ggtttgacaa accggtgcca aagtcatcaa
    34441 tcgatatccg taccccttgt ttacgcaatt gttcaatatt gagataaaaa tgttcattaa
    34501 ttcgttcgat tacggtttca gtgatctcaa cacagaggct gttcgccttc aggccatttt
    34561 ctttcataat ggtggtaaaa cgctcatgaa agtgtggttc gtttaactgc cagggcgaaa
    34621 cattaatatg cagtaagaaa tcatccttca gcgacgcacg ctgactaaaa tgtctgaatt
    34681 cacccattgc cgttttaata atctgataac ccagctcatt gataaacccg atatcttcag
    34741 caatggtaat gaacttcagc ggtgaaatga tcccccgctc cgcagacacc caacggcaca
    34801 acgcctccgc gcccagaatg gcgcgggtgt taatatcaac gatcggttga tagacgagga
    34861 atagttcgcc aatttccatt gcatggcgaa tattacgcgc aatccagata ttctcttcta
    34921 tttcgttgag tttcgcgtca tcacagaaaa taaactcgga gaacatatgg tcacggatac
    34981 tggaaatcgc cagtttcagt ttttctgcgt aactccaggc gttttccccc tgcaaaggtt
    35041 cacaaatggc agacttatgg atgcgcagat tgcggtagcc aaagctgctg tcaaaatcgt
    35101 ttaccatcgc tattttacgg gtaaatgtct gtacattttc ccgacacaga agtaaataga
    35161 gatcggcatt gaatgtcacg atatcgcagc attgtgcata tttctcggca attcgctgac
    35221 acaatttcct gacgatttta ttgcgttcat caacaccaaa caccgcttcc acggcttcca
    35281 gaccacacag tttgatgatc cccacagcca gatactgatt attgttggac tcaataatcg
    35341 ccatattttt aataaaccca ctttcctgat cgtaagagat cagtgagagc aatttatttg
    35401 accgttcaat ttcggcatct ttcatttccg cgatcgaatt tttgatgctg ataatctctt
    35461 tcgcatcgcc ttcgcgaatt ttcagagcct cgatctgatc cgttgagtca ggcaaaaacg
    35521 ccagcgtatt aaccagctct gtcatttcag aaaattgccg acggaacaca cgagtggtca
    35581 gtaaataaac caccagcaat acgataataa tgaacgcaaa cggcagataa aaaagtttat
    35641 ctgatgcgtt caataaatta ccgcccggat aataaaaata gatttcgaat aatgcgttat
    35701 ttggcaccgc tgatgttttt tcgatatatt cagtatcgac agaatcaata atattataat
    35761 taccttcgct attataaata atcagttttt cacgagcgag ttgattcttg gcaatgatct
    35821 catggccttg tttcaaaaat agaacttcat cattagcgct gatttgatca agatactcgt
    35881 tcagatatcg ccagtttaaa tcgacatgaa ttttatccac cgctaacaaa tcgtgatctt
    35941 tgccgttgat gcgtagcgta aaagagatac attttttacc cggacgatca aagcaattgt
    36001 atgccggatt ccagctgttg ttagagtcat ctgaagccac cgaccacggt cgtttcgatg
    36061 ggttaaaatc ggcaaaaata acccgccctg actcgtcgac aagcggtcca tttacttgtt
    36121 cctgatgata aacaacgatt ttatcgttcg ccctccgcga aaagagataa tatttattat
    36181 catcaagtac cagtcccacc gagtctaaaa atggcatcat ttccattttc aggatcacag
    36241 cgcgttgaat ctcctctgta ttttttaata aatacagtga gtcctttaat gtcagggccg
    36301 aacgttcaat atctttaaag aaatagccaa cgtttttctg aataagttca aagcggctat
    36361 cggcacgttt taccgcgcgt tccgatacca gttgatagtt aatgatgaaa aaaaggccaa
    36421 taaatgacag tgctgccaat aaaaataaaa ttatcagcct tatctttatc ttttcgtata
    36481 aactcagcat gttaatacat ttaacaaaat aactgtctga acgctgtgcc gctgcaaaaa
    36541 cagtcccttg ctgcggcttt taaccaaatt acccgattct acaaaatcag cagaaagtta
    36601 caacacttgc ttagggcaat aatttcctgt gaggtgatta ctctttcaag caatattcaa
    36661 acgtaactat cctttaattt tcggatccag cgcatcgcgt aaaccatcgc ccaacaaatt
    36721 gaacgccagt acagtcagaa aaatagccag ggccggaaaa acagcgacat gcggcgcgat
    36781 aaccatatcc gctcgagcct cattgagcat tgctccccac tctggtgtcg gcggctgcgc
    36841 accgaggccg agaaatgaca ggctggcggc agagataatc gaagtgccaa tgcgcatggt
    36901 gaaaaacacc acgatagacg agacggtccc aggcaagata tgacgcaata aaatggtcat
    36961 atcgctggca ccaatactgc gtgctgactc aataaaggtt tgctgtttca acaccagcgt
    37021 gttgccgcgc accaggcggg caaacgcggg gatggaaaaa atggcgactg caataatcac
    37081 gttagcaatg ccgcttccca acaccgcaac aacagcgatc gccagtaaaa tacccgggaa
    37141 ggcaaacagc acatcgcaaa tgcgcatgat cagccgatcc caccagcctt cataatatcc
    37201 agccagcaag cccagcaacg tccctatcgc cgcaccgata aacacggcaa acacgcccgc
    37261 agccagcgag atttgcgcac caaccaggac acggctgaaa atgtcgcgcc ccagtgaatc
    37321 gacgccaaac cagtgctgca aagaaggtcc gttattcaga ttgtcataat caaaataatt
    37381 ttcggcgtca tagggagcga tccagcgtgc aaaaatggcc accataatca ataaaataac
    37441 gaataatgcg gcggtcatcg ccatatgctg acggcgaaat cgtcgccaga attcatgcca
    37501 cggtgtacgt acctggtcag gtttgaccag tggcatggcg tttaacaccg cctgacgtcg
    37561 ccagttaaat agtcgcatcc ttacttgtac ctgatagccg ggttaatggc ggcgtaaagc
    37621 acatccacca ctaagttgat aagaataaat tccagcgaga aaagcagaat ttccgcctga
    37681 atcaccgggt aatcgcgcat ttctacggag tcaacgagta agcgtccaag tccgggccag
    37741 ttgaaaactt tctccaccac gatagaacca ccgagcaaaa agccaaactg taagcccatc
    37801 atggtcacta ccgggatcat cgcgttacgt agcccgtgtt tgaggacaac ccaggtttcg
    37861 ctcacccctt tcgccctcgc ggtacgcata taatcttcgc ttaaaacgtc gacaaacgac
    37921 gcgcgggtaa agcgcgccat cacggcggcc actgccgcgc cgagcgtcag ggagggtaaa
    37981 atgtagtgct gccaactgtc tgctcccacg gtaggcagcc agcccagttc aacggagaat
    38041 acctgaatta aaagcatccc cagagcaaat gccggaaacg agatccccga caccgcaatg
    38101 gtcatactca atcgatccgg ccaacgatta cgccagacgg cggcgataat tcccgccgcc
    38161 ataccaaata tcaccgccca gaccatactg gttatggtca gccacagcgt tggcataaag
    38221 cggctggcaa tctcatcggc aaccggacga cgcgacacca tcgacaggcc aaaatccccc
    38281 tgcacagcat tgctgatata gtgccagaac tggtgataca gcggctgatc caaccccagc
    38341 tgctgacgca ccagttctat aacctgcgca tcagcttcgg gcccggcaat caatcgcgcc
    38401 ggatcgccgg gcagcatatg cacaaataaa aacaccagca ccgagacgat aaacagcgtc
    38461 ggaatcaacc ccagtaatcg tttgataacg taattaagca ttccactccc tgcgttgctt
    38521 attgcaaatc cgcgtcttca aagctgaagc cggtgtctgg catgatccaa aaaccggtca
    38581 ggtttttact gtgtgccgac accagttttt ctaccaccag cgggatccac ggcgactctt
    38641 gccagatgat atcctgcgcc gccttataca agcgggtctt ttccgccgga tcattagttt
    38701 tcagtgcctg agccaggaag tcatccacct gtttattgct gtaaaacgcg gtattaaaca
    38761 gcgtcggtgg ccagttctgc gaggcaaaca gcggcgatag tgcccagtcc gcttcgccgg
    38821 ttgaagccga ccagccagtg tagaacatcc gcacgccgct ctctttttgc cctttacctt
    38881 caacttctgc cgcccgctgt ccggcatcca tcgcagtcac ctgggcttta atcccgacct
    38941 gcgctaactg ctgctgggta aattgcagca ctttctgcgc ggtgctgtgg ttatgtgacg
    39001 accacagcgt ggtattgaaa ccattgggat atcccgcctc tttcagtaat tcgcgcgctt
    39061 tcactggatc gtaaggccac ggtttataac tttgcgcgta ggcgatactc ggcggtacca
    39121 caccagtagc tggcgttgca tagcccgcga aggccacttt caccagcgcc ggacggttaa
    39181 tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa tggcttttgc gtcacgttca
    39241 tactgatata acgctgcata attgacggac tggccatcaa ctcgatattt ttgtttttct
    39301 ccagcagtgc ggcctgctcg taaggaatgg ggaaagcaaa ctgcgcttca ccggtttgca
    39361 gcattgccgc gcgggtgttg ttatccgcca ccggacgcca ggttatgctg tccagtttgg
    39421 gcaatcctgg ctgccagtaa cccgcgaatt ttttcacctt cacaaaatca gtctgattcc
    39481 aggtatccag ttcatacggt ccggttccca ccggatgaaa accaatctcc ttgccatatt
    39541 tttccagcgc tgccggtgaa atcatcgcgg tcgccggatg ggcaagaata ttaataaacg
    39601 ctgagaacgg ctgtttgagg gtaatcttta ccgttgtcgg atcgatcgct tccgttttag
    39661 caatattctt atacaggtta tagcgtttaa gatgatttgc cggatcgctg gcccggtcca
    39721 gattcgcttt caccgctgcg gcgttgaaat cggtgccatc ctggaattta atgccttccc
    39781 gcaatttcac ggtgtaagta atgccgtcat cggaaacggt ataactctcc gccagcacgt
    39841 ttttcagttt catctcttta tccagaccga acagcccctg gtaaaacgat ttcgctacgg
    39901 cctgagataa cgtgtcattc gcgtcatacg gatcgagcgt ggtgaaattc gatcctaccg
    39961 ccaccaccac atctttggca gcgaatgcac aagatgccat caacgctgtc gcaatgccca
    40021 gcgccactaa cccactacgg tgtacagctc ttgccatttt attctccgcc tgaatgttat
    40081 ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt aatgccccgg cccgacgcat
    40141 tgcaacgaga cgggtgcaac ctcttcgcca cgcagatgaa tattgctggg aagatcgtcc
    40201 gacagcagta cacgctgcgg tcgttgtcgg gacggttcag cgaccggaac tgccgccagt
    40261 aatttacgcg tataaggatg ctgcgggttt tcgaagaccg cgcgccgtgg accaatttca
    40321 acaatttgcc cgagatacat caccgccaca cgatgactaa tccgctctac cacagccata
    40381 tcgtgggaga taaacagata cgcaatgccg aaatcacgct ggagatcgag caacaagttg
    40441 ataatctgcc cgcgaataga aacatccagc gccgaaacgg cttcgtcggc aatgatcact
    40501 tttggattca atgccaacgc gcgagcaatg cagatgcgct ggcgctgacc gccggaaaac
    40561 tcatgcgggt aacgccaggc atgttcaggt aacaggccca cgcgctccag caaccacgca
    40621 acgcgtgcaa ccgcttcttt acctggcaat aaaccgtgta cacgcaacgg ttcgataatc
    40681 gaatcaccga tggtctgacg tgggtccagc gaagcgtaag ggtcctgaaa aataaactga
    40741 atatcgcggc gcaatacctg aagtttgccg ggtgacaagg tatcgattcg ctgaccgtta
    40801 aagataattt cgccgccctg cgattcgacc aggcgcagca acgcccgccc ggtagtggat
    40861 ttaccgctgc cagactcgcc caccagcgat agcgtttcgc caggccagag atcaaaactg
    40921 actttctcaa cggcatgcac ttcccgcgtt acgcgattca acaaaccgct gcgcaaaggg
    40981 aaacgggtga ccagattacg cacccgtaaa acaggttcgc catccaccac cgttttctgc
    41041 tcgatggggg gttcctgttt cgctggatgt tcaagcgata tcaacgggaa acgtcgggga
    41101 taatctaacc ctttcatcgc accaagttgc ggaacagcag ctaacagcgc acgggtgtaa
    41161 ggatgttgcg gtgcatgaaa aatctgttcg acggtacccg tttccaccgc ctcgccctga
    41221 tacatcacca gtacccgatc ggcaatctct gccaccacgc ccatatcgtg agtgataaag
    41281 ataacgccca tcgacatctc tttttgcaat actttgatta attgcaggat ctgcgcctga
    41341 atagtgacat ccagcgcggt ggttggctca tcggcaatca gcaccgccgg gcggcatgac
    41401 agcgccatcg caatcatcac tcgctggcgc atcccgccag agagttgatg cggataacgt
    41461 gaaagaatgg tttgtgcctc cggaatgcgt acctgatcca gcatccgctt cgcctcgacc
    41521 atcgcttctt cacgactggc gttttgatgc agacgaattg attcggcaat ctgttcaccc
    41581 acagtaaata ccgggttcag cgatgtcatc ggctcctgaa atatcatcgc catatccgca
    41641 ccgcgcacat gacgcatttg tgcagcgctc tgctcgctaa gttcaatcac ttcacgactg
    41701 cgccgccgca acagcatttt atcgcactgt actaaaccgc ccgcctgttc caacagacgc
    41761 atcaatgcca gcgcagtcac tgacttaccg gagccggatt cgccaacaat tgccagcgtc
    41821 tcaccgcgtt gcaggctaaa agagagattg cggaccgcag ctattttctg ctggtcctgc
    41881 ataaaggcaa tattaagatt ttcaaccgcc agcacattac cggcatcaag ttcatcactg
    41941 tgtggcaacg gcgtcccctt tttcacggta gataccggtg gttggcgtat cgcctgcgta
    42001 gccccaggcg cgatacattc cttcggtgtt aaacggtagc gcgacattcc cttcatggtc
    42061 gatagcgatt aagccaccgc taccgccaag cgcagggagt ttttccatta ctacccgctc
    42121 gcaggcttcc gcgagactta atccgccgta atccattaac gcggcgatgt catatgccgc
    42181 cagcgcgcgg atgaagactt cgcccgtgcc ggtacaagaa accgccacac tggcgttatt
    42241 ggcgtagcat ccggcaccca ctaaggggct atcgccaact cgtccgggta atttattggt
    42301 cattccgccc gtggacgtgg ctgccgccag attgccgtct aaatccaacg ccacggcccc
    42361 cacggtgccc attttctgtt tttcatccag tggcgcaccg ctatggtcga ggactgttgc
    42421 cccttcctcg cgcgctgcca ttagttgttc ataacgcaaa ggcgtggaga aaatctccgg
    42481 tgagacgcgc tccatgccat gagcaaacgc aaaattttct gccccttcgc caatcatcat
    42541 cacatgcgag ctttgctcca tcaccagccg ggcggcaaga accggattac gcagatgact
    42601 aacgcccgcc accgcaccgg ctttcagggt gttaccatcc atcacacagg cgtccagttc
    42661 atgcgtttca tcacgcgtaa agacggcgcc aattccggcg ttaaacagtg gacactcttc
    42721 cagcagacgc accgcttccg tcaccacatc cagcgcactt tcgcccgctt ccagcatttt
    42781 ctgcccggtt tcaacaatgg cagacaacgc ctcgatgtag cgtaattcct gttgcagact
    42841 catctgcgcg cggctaattg cacctgcgcc accatgaatt gcaatgactg ctttgcccat
    42901 agtattcgtc cattaagggt ggctcgccac ttttgctata aatatcagaa tttttatgaa
    42961 ttactcattc aatttttgaa tatagaaaga tggatatggc atgtaaaggc aggacccagc
    43021 gctacgcccg aggtggcata cagtctgtgg cgtttttctg acataatagg caaattcgat
    43081 tttgcctccg caggagtgtt ttcatggaat ttaccaccgg attgatgtcg ctcgacaccg
    43141 cgcttaatga gatgctttct cgcgtcaccc cactgaccgc ccaggaaacg ctgccactgg
    43201 tacagtgttt tggtcgtatt ctggcgagcg atgtcgtttc gccgcttgat gttccggggt
    43261 ttgataactc cgcaatggac ggctacgcgg tgcgtttagc cgatattgcc tccgggcaac
    43321 cgctgcccgt tgccggtaaa tcctttgccg gtcagccgta ccatggtgaa tggcctgcgg
    43381 gtacctgcat tcgtattatg accggtgcgc cggtgccgga aggctgcgaa gcggtggtga
    43441 tgcaggagca gactgaacaa acggacaatg gcgtgcgttt tactgctgaa gtgcgtagcg
    43501 ggcaaaatat tcgccgtcgc ggtgaagata tctctgcagg tgcggttgtt ttcccggcgg
    43561 ggactcgcct gactaccgca gagctgccag taattgcttc actggggatt gccgaagttc
    43621 cggtgattcg taaagtgcga gtggcgcttt tttctaccgg tgatgaactc cagttgcccg
    43681 gtcagccgct gggcgacggc caaatctacg ataccaaccg tctcgccgta cacctgatgt
    43741 tagaacagct gggttgcgag gtaattaact tagggattat ccgcgatgat ccccatgccc
    43801 tgcgcgccgc atttattgaa gccgacagcc aggcggatgt ggtgatcagt tccggcggtg
    43861 tttcagtggg tgaagcggat tacaccaaaa cgattcttga agagctgggg gagatcgcct
    43921 tctggaagct ggcgattaaa ccaggtaaac cgttcgcgtt cggtaaactc agcaatagct
    43981 ggttctgcgg cctgccgggc aacccagttt cagcgacgct gaccttctat caactggtac
    44041 agcctttgct ggcaaaacta agcggtaaca ccgccagcgg cctgcccgcg cgccagcgcg
    44101 tacgcacagc gtcccgcctg aaaaaaacgc caggtcgcct tgatttccag cgcggcgtgc
    44161 tgcaacgcaa cgccgatggc gaactggaag tgacgaccac tggacatcag ggttcacata
    44221 tatttagctc ctttagcctc ggcaactgct ttatcgtact ggaacgcgat cgcggcaatg
    44281 tggatgtggg cgaatgggtg gaagtagaac cgtttaacgc gttgttcgga ggcctgtaat
    44341 ggcggaactc agcgatcagg agatgctgcg ctacaaccgg caaatcattc tgcgcggttt
    44401 tgattttgac ggtcaggagg cgctaaaaga ttctcgtgtg ctggtagtcg gcctcggtgg
    44461 actcggctgc gcagcttcgc agtatctggc aagcgccggt gtcggcaacc tgacgctgct
    44521 cgacttcgac tcggtttcgc tctcgaatct gcaacgccag acgctgcaca gtgatgccac
    44581 ggtcgggcaa ccgaaggtgg aatccgcccg tgacgccctg gcgcggatca acccacatat
    44641 cgcgattacg ccagtcaatg cactgctgga tgacgcagaa cttgcagcat tgattgctga
    44701 acacgatctg gtgctcgact gtacggataa cgttgcggta cgtaatcaac tgaacgcagg
    44761 atgttttgcc gcgaaggtgc cgctggtttc cggcgcggca attcgtatgg aaggtcaaat
    44821 cacggtcttc acttatcagg acggcgaacc gtgctatcgc tgccttagcc gtttgtttgg
    44881 cgagaacgcg ttaacctgcg tagaagcagg cgtgatggca ccattgatcg gcgtaattgg
    44941 ttcgttgcag gcaatggaag cgatcaagct gctggcaggt tatggcaaac cggccagcgg
    45001 aaaaatcgtg atgtacgatg cgatgacctg tcagtttcgc gaaatgaaac tgatgcgcaa
    45061 tccggggtgt gaagtgtgcg ggcagtaatt gccacctcca ggcctgataa gacgcggcac
    45121 tccgccacgt catcagcaat ttatgaggat gcagaacgtc cggttaaatc gacgttctgc
    45181 caaacgctcc ctgccagtcc tgctcaaact tcgccacagc ggcatcaacc gccggatagc
    45241 taatcatctg ttgtgccaca tccagtggca gagtaattga ttcacatcct gccagtaagc
    45301 agtccagcgc ctgacgcggg gttttgaaac tcgctgccag cactttcgcc tgcggcgcat
    45361 gcattttcaa taactggtgt aagtcggtca cagtctgaat gccgctaccg ccctgagcat
    45421 caatacgatt aacgtaaggc gcaacatatt ccgcacctgc cagcgccgac agcaaccctt
    45481 gtgccgcgcc atataccgcg gttcccagcg tcggaatccc ttccgctttt aacatcttaa
    45541 tcgctgccag cccctcggcg gtcaccggaa ctttcaccac gatatccgca ataatagaac
    45601 gcaatttacg tgcgtcattc accatccctt cagcagtgat agccattacc tgggcaaaca
    45661 gacgcccctg accgcccatc gcttcatgaa gttccggaag cacaacctcc agcggttttt
    45721 tacccgcggc gataatgctt gggttagtgg tcacgcccgc cagcggaaaa atacgtgaca
    45781 gcgccttcac cgcaacaacg tctgaagtat ccagatacag ttccataacc atcctcaaaa
    45841 tgtacttttt gtctgaatgt agactatcac gggaaaactc tctgaacagt tgacgcacat
    45901 caatctaact ttcattcgaa agtattttaa tgtttgaacg aaacagagag gcaactatga
    45961 ttttcaatat tcagcgctac tcgacccatg atggccccgg tatccgcacg gtcgtatttc
    46021 ttaaaggctg ttcgctgggc tgccgctggt gtcagaaccc ggaaagccgc gcccgcacgc
    46081 aggatctgct gtatgacgca cgactgtgtc tggaaggctg cgagctgtgc gctaaggcag
    46141 cgccggaagt gattgagcgc gcgctgaatg gtttgcttat tcatcgggaa aagttaaccc
    46201 cggagcatct gacggcgtta accgactgct gcccgacaca ggcattaacc gtgtgtggtg
    46261 aagtgaaaag cgttgaggag atcatggcga ccgttttgcg cgataaaccg ttttacgatc
    46321 gcagcggcgg aggcttaacg ctttcgggtg gtgaaccctt tatgcagccg gaaatggcga
    46381 cggcgctact gcaagccagc cacgaggccg gcattcatac tgcggtagaa acctgtctgc
    46441 atgtgccgtg gaaatatatc gccccttctc tgccctatat cgatctgttt cttgccgatt
    46501 taaaacacgt tgccgacgcg ccgtttaaac agtggaccga cggtaacgcc gccagagtgc
    46561 tggataacct gaaaaaactc gccgcagcgg gcaaaaaaat cattatccgc gtgccgctga
    46621 ttcagggctt taatgccgac gaaacctctg taaaagccat taccgatttt gccgccgacg
    46681 agctgcacgt tggcgaaatt catttcctgc cctaccacac gctgggcatc aacaaatatc
    46741 acttacttaa tctgccctat gacgccccgg aaaaaccgct tgatgcgcca gaactgctcg
    46801 actttgccca gcagtatgcc tgccagaaag ggttaaccgc gaccttacga ggataacaac
    46861 catgaccaca ctgaaactgg acacgctcag cgaccgcatt aaagcgcaca aaaatgcgct
    46921 ggtgcatatt gtgaaaccgc cggtttgtac cgagcgcgcg cagcactata ccgagatgta
    46981 tcaacaacat ctcgataagc cgatccctgt acgtcgcgcg ctggcgctgg cacatcacct
    47041 ggcgaatcgc actatctgga tcaagcacga tgagttgatc atcggcaacc aggcaagcga
    47101 agttcgcgcc gcgccgatct tcccggaata tactgtctcg tggatcgaaa aagagattga
    47161 tgatctggca gatcgtcccg gtgctggctt tgcggtgagc gaagagaaca aacgcgttct
    47221 gcatgaagtg tgcccgtggt ggcgcggtca gaccgtacag gatcgctgct acggcatgtt
    47281 taccgatgag caaaaaggtc tgctggcgac cggcatcatt aaagcggaag gcaatatgac
    47341 ctccggcgat gcgcacctgg cggtcaattt cccgctgctg ctggaaaaag ggcttgatgg
    47401 tctgcgcgag aaagtagcgg aacgtcgctc gcgcatcaac ctgacggtgc tggaagattt
    47461 acacggcgag cagttcctga aagcaattga tatcgtgctg gtggcagtca gtgaacacat
    47521 tgaacgtttc gctgccctgg cgcgtgaaat ggccgcgacc gaaacccgcg aaagccgtcg
    47581 cgatgaactg ctggcgatgg cagaaaactg cgatcttatc gcccaccagc cgccgcagac
    47641 gttctggcag gcgctgcaac tgtgttactt catccagttg atattgcaga tcgaatctaa
    47701 cggtcactcg gtatcgtttg gtcgtatgga ccagtatctc tacccttact atcgccgcga
    47761 cgttgaactc aaccagacac tggatcgcga acacgccatc gaattgctgc atagctgctg
    47821 gctgaaactg ctggaagtga acaaaatccg ctccggctca cactcaaaag cctctgcggg
    47881 aagtccgctg tatcagaacg tcaccattgg cgggcaaaat ctggttgatg gtcaaccaat
    47941 ggacgcggtg aatcccctct cttacgcgat ccttgaatcc tgcggtcgcc tgcgttccac
    48001 tcagcctaac ctcagcgtgc gtttccatgc aggaatgagt aacgatttcc tcgacgcctg
    48061 cgtacaggtg atccgttgcg gcttcgggat gccggcgttc aacaacgacg aaatcgtcat
    48121 cccggaattt attaaactcg gtattgaacc gcaggacgct tacgactatg cagcgattgg
    48181 ttgtatcgaa accgccgtcg gtggcaaatg gggctatcgc tgtaccggca tgagctttat
    48241 caacttcgcc cgcgtgatgc tggcggcgct ggaaggcggg cgtgatgcca ccagcggcaa
    48301 agtgttcctg ccacaagaaa aagcgttgtc ggcaggtaac ttcaacaact tcgatgaagt
    48361 gatggacgcg tgggatacgc aaatccgtta ctacacgcgt aaatcaatcg aaatcgaata
    48421 tgtcgtcgac accatgctgg aagagaacgt gcacgatatt ctctgctcgg cgctggtgga
    48481 tgactgtatt gagcgagcga aaagtatcaa gcaaggcggc gcgaaatatg actgggtttc
    48541 tggcctgcag gttggcattg ccaacctcgg caacagcctg gcggcagtga agaaactggt
    48601 gtttgaacag ggcgcgattg gtcagcaaca gctggctgcc gcactggcgg atgacttcga
    48661 tggcctgact catgagcaac tgcgtcagcg gctgattaac ggcgcgccga agtacggcaa
    48721 cgacgatgat accgtcgata cgctgctggc tcgcgcttat cagacctata tcgacgaact
    48781 gaaacagtac cataacccgc gctacggtcg tggtccggtt ggcggcaact attacgcggg
    48841 tacgtcgtct atctccgcta acgtaccgtt tggcgcgcag actatggcaa cgccggacgg
    48901 acgtaaagcg cataccccgc tggcagaagg cgcaagcccg gcttccggta ctgatcatct
    48961 tggccctact gcggtcattg gctcggtggg taaactgcct acggcagcga ttctcggcgg
    49021 cgtgttgctc aaccagaaac tgaatccagc cacactggag aacgaatctg acaagcagaa
    49081 actgatgatc ctgctgcgca ccttcttcga ggtgcataaa ggctggcata ttcagtacaa
    49141 catcgtttcc cgcgaaacat tgctggaggc gaaaaaacat ccggatcaat accgcgatct
    49201 ggtagtgcgt gtcgcgggct attccgcctt cttcaccgcg ctctctccag acgcacagga
    49261 cgatatcatc gcccgtactg aacatatgct gtaatctcct cggcccggcg gcgtcgtcgc
    49321 cgggctaaat cattcacatc atcaatttca tccttacttt cattcgaaat ataatttgtg
    49381 ctctgcgtca cattgttatt acactgttac ggtttcattt tcgagccagg agcacaccta
    49441 tgagcgtaaa agttatcgtc acagacatgg acggtacttt tcttaacgac gccaaaacgt
    49501 acaaccaacc acgttttatg gcgcaatatc aggaactgaa aaagcgcggt attaagttcg
    49561 ttgtcgccag cggtaatcag tattaccagc ttatttcgtt ctttcctgag ctaaaggatg
    49621 agatctcttt tgtcgcggaa aacggcgcac tggtttacga acatggcaag caactgttcc
    49681 acggcgaact gacccgacat gaatcgcgga ttgtgattgg cgagttgcta aaagataagc
    49741 aactcaattt tgtcgcctgc ggtctgcaaa gtgcatatgt cagcgaaaac gcccccgaag
    49801 cattcgtcgc actgatggca aaacactacc atcgcctgaa acctgtaaaa gattatcagg
    49861 agattgacga cgtactgttc aagttttcgc tcaacctgcc ggatgaacaa atcccgttag
    49921 tgatcgacaa actgcacata gcgctggatg gcattatgaa acccgtcacc agtggttttg
    49981 gctttatcga cctgattatt cccggtctac ataaagcaaa cggtatttcg cggttactga
    50041 aacgctggga tctgtcaccg caaaatgtgg tagcgattgg cgacagcggt aacgatgcgg
    50101 agatgctgaa aatggcgcgt tattcctttg cgatgggcaa tgctgcggaa aacattaaac
    50161 aaatcgcccg ttacgctacc gatgataata atcatgaagg cgcgctgaat gtgattcagg
    50221 ctgtgctgga taacacatcc ccttttaaca gctgacctct ctaccggagc catgcgctcc
    50281 ggttttccct tctttttaca tccaacacat caaatcatta aacttgcatt aacttattta
    50341 taacttatat tgtcacatgc aaatcagcac agtttcgaat gaaagttaat gcgaggtggt
    50401 tatggctcct acttttacca gcgacacatt gcctgccgat cacaaagcag ctatccgtca
    50461 gatgaagcac gcgctgcggg cgcagcttgg cgacgtccag cagatcttta atcagctaag
    50521 cgatgacatt gccacgcgag tggctgaaat caacgcactc aaagcacagg gcgatgccgt
    50581 ctggccggtg ctgtcttatg ccgatatcaa agcaggtcat gttactgcag agcagcgcga
    50641 acagattaaa cgtcgcggtt gtgcggtgat aaaaggccat ttcccccgcg aacaagcgct
    50701 aggctgggat cagtcgatgc tggactatct ggaccgcaac cgctttgacg aggtctacaa
    50761 aggccccggc gataatttct tcgggacgct cagcgcttca cgtcccgaga tttaccccat
    50821 ctactggtcg caggcgcaaa tgcaggctcg ccagagtgaa gaaatggcga atgcgcagtc
    50881 gtttctcaat cgtctgtgga catttgaaag tgatggaaag caatggttta acccggatgt
    50941 gagcgtcatc taccctgacc gtatccgccg ccgtccgccc ggaacgacct ccaaaggtct
    51001 tggagcgcat accgactccg gggcactgga acgctggctg cttccagcgt atcagcacgt
    51061 tttcgctaac gtctttaatg gcaatctggc gaaatacgat ccctggcatg cggcacatcg
    51121 tacggaagtt gaagagtaca cggtagataa cactacaaaa tgttcggtgt tccgcacgtt
    51181 tcaaggctgg acggcgctct ctgatatgtt atcgggtcag ggattgttac atgtggtgcc
    51241 tatcccggaa gcgatggcgt atgttttatt gcgcccgctg ctggatgacg taccggacga
    51301 tgaactatgc ggggttgcac ctggcaaagt tctgcccatt tcagagaaat ggcatccact
    51361 actgcttaaa gcgctgactt ccattcccgc gataacagca ggggattccg tctggtggca
    51421 ctgcgatgtg attcactccg tcgcgccagt agacaatcaa cagggttggg gcaacgtgat
    51481 gtatatccct gctgcgccaa tgtgcgagaa aaatcgggct tatgcacaca aagtcaaaat
    51541 ggcgctggaa aaaggcgcat cgccgggcga cttcccgcgc gaggactatg aaacaaactg
    51601 ggaaggacgc tttacgcttg ccgacctcaa cattcacggt aagcgcgcac tgggcatgga
    51661 tgtttgagaa ttaatggaag tgaattgatg tgggattaag aaaatcgatt cgacgttact
    51721 cagattgctg acaagtgcgc tttcttaatg cctgatgcgg cgttaacgcc ttatctggcc
    51781 tacaaagtcc tgcagattca atatattaca ggtactgcgt aggcctgata agcgtagcgc
    51841 atcaggctat tttgcatttg tcatcagccg ccgtcacccc gacagtatcg gggtgacgga
    51901 taataactta ctcgatccct ttactacgca ggtaatcttc gtaattaccg ctaaagtcga
    51961 tcacgcgttc cggggtgatt tccagaatgc gggtcgccag ggagcttacg aactcacggt
    52021 cgtgtgaaac aaagatcagc gtgccctgat acagttccag cgccatgttc agtgactcga
    52081 tggattccat atccaggtgg ttggtcggtt cgtccatgat cagaatgttc ggcttctgca
    52141 tcattaactt accaaaaagc atccgccctt tttctccacc ggaaagcact ttagctggct
    52201 ttttgatgtc gtcctggctg aacagcaaac gaccgagaat gctgcgtacc gcctgctcgt
    52261 catcgccttc ctgcttccac tggctcatcc attcgaacac ggtcagatca ttttcaaact
    52321 catattcgtg gtcctgagca tagtaaccaa tgcgcgcgtt ttcagaccat tttacggtgc
    52381 cgctgtccgg ttgcagatca cccaccagcg ttttcagcag cgttgattta ccgacgccgt
    52441 tggtacccag taccgcaagt ttttcaccga cctccagcag caggttgaga tttttaaaca
    52501 gtggaccgtt atcaaaccct ttggtcagac cttccacttc cagcgcgtta cggaacagtt
    52561 tcttatcctg ttcaaaacgg atgaacgggt tctgacggct ggaggctttc acctcttcca
    52621 gtttgatttt atcaatctgg cgcgcgcgcg aagttgcctg gcgagatttc gaggcgttgg
    52681 cgctaaagcg gctaacgaaa gattgcagct cagcaatctg cgctttcttc ttggcgttat
    52741 cagccagcag acgttcacgc gcctgggtcg ccgccgtcat gtactcatcg tagttgcccg
    52801 gataaacgcg cagctcgccg taatccagat ccgccatgtg ggtacaaacc atgttaagga
    52861 agtgacggtc gtgcgagatg atgatcatgg tgctgtcacg ctcgttcagc acctgttcca
    52921 gccagcgaat ggtgtcgatg tcgaggttgt tggtcggttc gtcgagcagg agaatatccg
    52981 gatcagcaaa cagcgcctgc gccagcagca cacgcagctt ccagccagga gcaacttcac
    53041 tcatcgggcc gtagtgctgt tccactggaa ttcccacgcc aagcaacaat tcaccggcgc
    53101 gagcttccgc agagtaaccg tccatttcgc cgtatttaac ttccagatcg gccactttat
    53161 agccgtcttc ttcactcatt tccggcaaag catagatgcg gtcgcgctcc tgcttcactt
    53221 cccacaactc tttatgcccc atgatcaccg tatccagcac agtgaactct tcaaaggcaa
    53281 actgatcctg acgcagctta ccaatgcgct cgttgggatc gagggaaacg ttacccaacg
    53341 tcggctctaa gtcgccgccg aggatcttca taaaggtgga tttaccacta ccgttcgcgc
    53401 caatcaggcc gtaacggttg ccgccgccaa atttgacgga aatgttttca aacaacggct
    53461 tactgccgaa ctgcatggtg acgttactgg aaactaacac aggcgtatcc tgaaaagaga
    53521 tatgacaaac cgcgcattat gccataagtc tgaaagaaga tcctgctcct cgcttaaggt
    53581 ctacactcaa gccaaaggca aaaaatgtga tttcgtacac atctgatttc actgtgagct
    53641 ggaatgaact tataatgcgc ttccaatact ctaatattct caacccaatg gcctgccagg
    53701 cacaaaatct cgcttaacat gaatatgaaa ttgaaaacat tattcgcagc ggccttcgct
    53761 gttgtcggct tttgcagtac cgcctctgcg gtaacttatc ctctgccaac cgacgggagt
    53821 cgcctggttg gtcagaatca ggtgatcacc attcctgaag gtaacactca gccgctggag
    53881 tattttgccg cggagtacca gatggggctt tccaatatga tggaagcgaa cccgggtgtg
    53941 gataccttcc tgccgaaagg cggtactgta ctgaacattc cgcagcagct gatcctgccg
    54001 gataccgttc atgaaggcat cgtcattaac agtgcagaga tgcgtctgta ttactatccg
    54061 aaagggacca acaccgttat cgtgctgccg atcggcattg gtcagttagg caaagatacg
    54121 cctatcaact ggaccaccaa agttgagcgt aagaaagcag gcccgacctg gacgccgacc
    54181 gccaaaatgc acgcagagta ccgcgctgcg ggcgaaccgc ttccggctgt cgttccggca
    54241 ggtccggata acccgatggg gctgtatgca ctctacatcg gtcgcctgta tgctatccat
    54301 ggcaccaacg ccaacttcgg tatcggcctg cgtgtaagtc atggttgtgt gcgtctgcgt
    54361 aacgaagaca tcaaattcct gttcgagaaa gtaccggtcg gtacccgcgt acagtttatt
    54421 gatgagccgg taaaagcgac caccgagcca gacggcagcc gttatattga agtccataat
    54481 ccgctgtcta ccaccgaagc ccagtttgaa ggtcaggaaa ttgtgccaat taccctgacg
    54541 aagagcgtgc agacagtgac cggtcagcca gatgttgacc aggttgttct tgatgaagcg
    54601 attaaaaacc gctccgggat gccggttcgt ctgaattaat cgtcaaaacg taaaacaaag
    54661 gcggactcat aatccgcctt ttttatttgc cagaccttac caggccggga tcataaccaa
    54721 caaaacatat cccaccagcg ccgcccacaa cagcatcggt attgaataga gatggaaacg
    54781 ccaccagatg cggcgatcgt tcgccatacg tagcgcaatc aaatttgcca gcgatccggg
    54841 taataaccca aagccaccta cgtttaccgc ccatgccagt aataaagacg gcggcacata
    54901 gttcagcaac aatatggtac tcggcacgtt actgatcacc tgcgataaac cgattgccgt
    54961 taaccataac ccgggttcag ataaatgact cacgttaccc aacacgcctt gcaacgctgg
    55021 aagctggatc agtaaatgga cgtcgataaa catcgccata aacaccagca gcagcgtcca
    55081 gtccacactc aacaccacgc gacgtgccag cagcgcaaag cccaccgcca caatcaccag
    55141 tccccacagc tcttgtttta actccagcgc cgtcagaaag acgatataca gtcccagaca
    55201 actccacacc agccgcggtt tccactccgg cgtttgcacc cccgtatggt attgcagcgc
    55261 ctttccaggg aaacaacacc agcacaacag caggagtgtc agcatcattg caccagccag
    55321 cggtgccatt tgggcaataa atccggcaaa cgaaagacca gaacgtcccc agataagaat
    55381 attttgcggg ttgccaattg gcgtcagtag cgaaccagca ttgactgcca gcgcctcaaa
    55441 aataatcagc cgattaaccg ggatctcaca cagtcttttt agcgtgatag tcagcggaac
    55501 aacaataaac agcgcgacat cgttggtcag aaaggtagaa agcagcgccg ccgccagcac
    55561 cataaacatc gccagccgac gctccgtagc aaagcggcgc accattttgc gccccagcac
    55621 atcaaaataa ccgcttaact ccacaccttt ggtcagcagc atcaggccgc ttaatgtgat
    55681 gatggtgtgc cagtcgatag cagcaggcca ggatttcggt gcaaagggca cgaaaaagct
    55741 taatccgata ccaacaagaa ttaataactg aaaaaaacga tccccttgca gcgtgcgtaa
    55801 aaaaggcagg ctcatttggc accgtgtttc tgggtaaaca aacgaaaggc atccagcgtc
    55861 tcttcactaa catggtgctc catgccttcc gcgtcgcgac gggcgatttc cggactgacg
    55921 cccaacacca ataagaaatt ttcgactatc tgatgtcgct cgcggctttc ctgcgccagc
    55981 ttctctcctt ctgccgttaa aaacacgcct cgccagggga tcatttcaat cagccccatg
    56041 gttgccagtc gcttaagcat tttagccacc gtcggttgcg aaactcccaa acgagcagcc
    56101 atgtctacct gacgagcttc ccccacttcc ctgatcaagt cagaaatcag ctcaacgtaa
    56161 tcatcaataa gctcgcgccg atgcgcctct ctgacctggc ggaacccttc aacgtgctct
    56221 tccacgttca ctaactgcgt cacttttttt gctgttggcg tacctgcgcg acgactcatt
    56281 gtgcttcctc attatggtga cgcatacata gcgttctcat ttcgtgcgca cattgtaaac
    56341 cagagttgcg aaggtacaaa aaattaacgt tttagcaata gctatataat atagcctgtg
    56401 ctatatctgt atgtaatgca atcatccctc aaggatcgac gggattagca agtcaggagg
    56461 tcttatgaat gagttcaaga ggtgtatgcg cgtgtttagt cattctccct ttaaagtacg
    56521 gttaatgctg ctctctatgt tgtgcgatat ggtcaacaac aaaccgcagc aagataaacc
    56581 ttccgataaa tagcggcgtc gcggtacgcc gcttcactcc tgctttcatg caggcataac
    56641 gcgttttggt ctgaaaaacc ccactttttg tcggatttgc aatccccttc gcaaaagatt
    56701 tgttcgtcag tagttgacct gaacggcggc tcgctctatc ttcttgcagc cctgcgtata
    56761 ttgcggctcg cggatgcgga cccctttccc ctcttcacgc actcttgcag gtattgaccc
    56821 ttgacgccag ggtaagcaca tggcgtttgt tacgatagtg gcatatgaat ttaaccctca
    56881 aagaatcgct tgttacccgt agccgggtat ttagcccgtg gactgcgttc tactttttac
    56941 agtcgctatt aattaacctc ggcttaggtt atcccttcag tttgctctac accgctgcgt
    57001 ttacggctat tttgcttttg ctatggcgaa cattgcctcg cgtacaaaaa gttctggtcg
    57061 gtgtcagttc gctggtggcg gcttgctatt tcccttttgc tcaggcctac ggcgcgccta
    57121 atttcaatac attgctggca ttgcactcca ccaatatgga agagtcgacc gaaatcctga
    57181 cgatttttcc gtggtacagc tacctggtcg gcttatttat ttttgcgctc ggcgtaatag
    57241 caatcaggcg aaaaaaagag aatgaaaaag cgcgctggaa taccttcgac agcctgtgtc
    57301 tggtattcag tgtggcgaca ttttttgttg ctcccgtgca aaacctggcc tggggtggcg
    57361 tatttaaact gaaagatact ggctatccgg tatttcgttt tgctaaggat gtcatcgtca
    57421 ataataacga ggtgattgaa gagcaagaac ggatggcaaa actttccgga atgaaagata
    57481 cctggacggt cactgccgtt aagccgaagt atcagaccta tgtggtggtg atcggtgaaa
    57541 gcgcgcgtcg cgatgccctc ggtgcctttg gcggtcactg ggacaatacc ccgtttgcca
    57601 gcagcgttaa cggtttgata tttgctgact acattgccgc cagtggctcc acgcagaaat
    57661 cgcttggctt aacgctcaat cgcgttgtcg atggcaagcc acagtttcag gataactttg
    57721 tcaccctggc aaatcgcgcg ggcttccaga cctggtggtt ttccaaccag ggccaaatcg
    57781 gcgaatacga taccgccatc gccagcatcg ccaaacgagc agatgaagtg tacttcctta
    57841 aagatggtaa ttttgaagca gataaaaaca ccaaagacga agcgttactg gatatgaccg
    57901 ctcaagtgct ggcgcaagag cactcgcaac cgcagctgat tgttctgcat ctgatgggct
    57961 cgcatccgca ggcctgcgac cggacacaag gcaaatatga aacttttgtg caatcgaaag
    58021 aaacgtcgtg ctatctctat actatgacgc aaacggacga tttactgcgc aagctgtacg
    58081 atcagttacg caacagcggc agcagcttct cgctggttta cttttctgac cacggtctgg
    58141 cctttaaaga gcgcggcaaa gacgtgcaat accttgccca tgatgataaa tatcagcaaa
    58201 atttccaggt gccttttatg gtcatttcca gcgacgataa agcgcatcgt gtgattaaag
    58261 cccgccgctc agccaatgac ttcttaggct ttttctccca gtggacggga attaaagcga
    58321 aggaaattaa catcaaatac ccgtttatat ctgagaagaa agccgggccg atatacatca
    58381 ccaacttcca gttacagaag gtggattaca accatctcgg aaccgatatt ttcgacccga
    58441 aaccttaaaa acaaaaatcc gccccgagag gcggattttt ttatatcacc aaagtgatta
    58501 gaagcggtaa ccaacaccgg caatccaggt gcctacgtca acgctacgaa tacggctctg
    58561 ctcgtaagag aagtccagag caacgttttc catcgggttg aactgcagac cagcaccgta
    58621 ggagaaaccg tagtcgctgg tgtcgtgttt gtaggtcggg tattcagtgg tctggaattt
    58681 accataaccc acacccacta caccgtagat gcttgcccag tcgttaatgc ggtaagccgg
    58741 accagcagtg atgccgtagt actggttttt gttgtagtca ccagagcttg cagtacggct
    58801 tttctcggtg taagtgaaag aaccgatcac acccagcggg ctgttgtctt cttcatagcg
    58861 gtatttcagg ttgaaaccgc ccattttgtt catttggccc tgagcgtcgc tctgtgcgta
    58921 accgccagtt acggtagaag tcgcagctac ggaagtacct gcggtgaaag ccagaactgc
    58981 ggccagtgct gaaagacatg caattttttt cataaccacc tcaaatgtga ttcaaataag
    59041 tcctaagttt taaatatatc aaaaattaat gggaaactct tcgcgatttg tgatgtctaa
    59101 cgggccattt catgtaacag aacgtttcca tacaccgcta tccatctaaa tttaaatcac
    59161 tttttcagag aactgcgtaa gtattacgca tgttttccct gtcattcatc cagattattc
    59221 ctaatcacca gactaatgat tccatcaatc ctggcgcatt ttagtcaaaa cgggggaaaa
    59281 ttttttcaac aaatgctcaa ccagcattgg gtatatccag tacactccac gctttactta
    59341 agtctagata tttgtgggag aaaggatgcc tggttcatta cgtaaaatgc cggtctggtt
    59401 accaatagtc atattgctcg ttgccatggc gtctattcag ggtggagctt cgttagctaa
    59461 gtcacttttt cctctggtgg gcgcaccggg tgtcactgcg ctgcgtctgg cattaggaac
    59521 gctgatcctc atcgcgttct ttaagccatg gcgactgcgc tttgccaaag agcaacgatt
    59581 accgctgttg ttttacggcg tttcgctggg ggggatgaat tatctttttt atctttctat
    59641 tcagacggta ccgctgggta ttgcggtggc gctggagttc accggaccac tggcggtggc
    59701 gctgttctct tctcgtcgcc cggtagattt cgtctgggtt gtgctggcgg ttcttggtct
    59761 gtggttcctg ctacctctgg ggcaagacgt ttcccatgtc gatttaaccg gctgtgcgct
    59821 ggcactgggg gccggggctt gttgggctat ttacatttta agtgggcaac gcgcaggagc
    59881 ggaacatggc cctgcaacgg tggcaattgg ttcgttgatt gcagcgttaa ttttcgtgcc
    59941 aattggagcg cttcaggctg gtgaagcact ctggcactgg tcggttattc cattgggtct
    60001 ggcggtcgct attctctcga ccgctctgcc ttattcgctg gaaatgattg ccctcacccg
    60061 tttgccaaca cggacatttg gtacgctgat gagcatggaa ccggcgctgg ctgccgtttc
    60121 cgggatgatt ttcctcggag aaacactgac acccatacag ctgctggcgc tcggcgctat
    60181 catcgccgct tcaatgggtt ctacgctgac agtacgcaaa gagagcaaaa taaaagaatt
    60241 agacattaat taaatttaca tttctgcatg gttatgcata accatgcaga atttctcgct
    60301 acttttcctc tacaccgtct ttatatatcg aattatgcaa aagcatattt attccgaaaa
    60361 ttcctggcga gcaaataaat aagaattgtt cttatcaata tatctaactc attgaatctt
    60421 tattagtttt gtttttcacg cttgttacca ctattagtgt gataggaaca gccagaatag
    60481 cggaacacat agccggtgct atacttagtc tcgttaatta ctgggacata acatcaagag
    60541 gatatgaaat tatgagtacc gctaaattag ttaaatcaaa agcgaccaat ctgctttata
    60601 cccgcaacga tgtctctgac agcgagaaaa aagcaacagt agagttgctg aatcgtcagg
    60661 ttatccagtt tattgatctt tctttgatta ccaaacaagc gcactggaac atgcgcggcg
    60721 ctaacttcat tgccgtacat gaaatgctgg atggcttccg cactgcactg atcgatcatc
    60781 tggataccat ggcagaacgt gcagtgcagc tgggcggtgt agctctgggg accactcaag
    60841 ttatcaacag caaaaccccg ctgaaaagtt acccgctgga catccacaac gttcaggatc
    60901 acctgaaaga actggctgac cgttacgcaa tcgtcgctaa tgacgtacgc aaagcgattg
    60961 gcgaagcgaa agatgacgac accgcagata tcctgaccgc cgcgtctcgc gacctggata
    61021 aattcctgtg gtttatcgag tctaacatcg aataaatcca tcgctgatgg tgcagaactt
    61081 tagtacccga taaaagcggc ttcctgacag gaggccgttt tgttttgcag cccgcctcaa
    61141 cgcacttatt tagtgcatcc atctgctatc cccagctgat taagtaaatt ttttgtatcc
    61201 acatcatcac acaatcgtta cataaagatt gttttttcat caggttttac gctaaataat
    61261 cactgtgttg agtgcacaat tttagcgcac cagtttggtg ccccagaatg gtgcatcttc
    61321 agggtattgc cctataaatc gtgcatcacg tttttgccgc atctcgaaaa atcaatgagt
    61381 tgcaaaactg gcacgatttt ttcatatatg tgaatgtcac gcaggggatc gtcccgtgga
    61441 tagaaaaaag gaaatgctat gaagtctgta ttaaaagttt cactggctgc actgaccctg
    61501 gcttttgcgg tttcttctca tgccgcggat aaaaaattag ttgtcgcgac ggataccgcc
    61561 ttcgttccgt ttgaatttaa acagggcgat aaatatgtgg gctttgacgt tgatctgtgg
    61621 gctgccatcg ctaaagagct gaagctggat tacgaactga agccgatgga tttcagtggg
    61681 atcattccgg cactgcaaac caaaaacgtc gatctggcgc tggcgggcat taccatcacc
    61741 gacgagcgta aaaaagcgat cgatttctct gacggctact acaaaagcgg cctgttagtg
    61801 atggtgaaag ctaacaataa cgatgtgaaa agcgtgaaag atctcgacgg gaaagtggtt
    61861 gctgtgaaga gcggtactgg ctccgttgat tacgcgaaag caaacatcaa aactaaagat
    61921 ctgcgtcagt tcccgaacat cgataacgcc tatatggaac tgggcaccaa ccgcgcagac
    61981 gccgttctgc acgatacgcc aaacattctg tacttcatca aaaccgccgg taacggtcag
    62041 ttcaaagcgg taggtgactc tctggaagcg cagcaatacg gtatagcgtt cccgaaaggt
    62101 agcgacgagc tgcgtgacaa agtcaacggc gcgttgaaaa ccctgcgcga gaacggaact
    62161 tacaacgaaa tctacaaaaa atggttcggt actgaaccga aataataacg ctacacctgt
    62221 aaagcgcact ggcagttccc tctcccctct ggggagagga ttagggtgag gggcgcaaac
    62281 ccgctccggg gccattaatt accctgaatt tgattattta caccacggta acaggaacaa
    62341 catatgcagt ttgactggag tgccatctgg cctgccattc cgcttctgat tgaaggtgcc
    62401 aaaatgaccc tgtggatttc ggtcctcggt ctggctggcg gtctggtaat cggattgctg
    62461 gcaggttttg cacgcacctt cggtggttgg atagccaacc acgtcgcgct ggtctttatt
    62521 gaagtgatcc gcggcacacc tatcgtcgtc caggtgatgt ttatttattt cgccctgccg
    62581 atggcgttta acgacttacg catcgaccca tttactgcgg cggtggtcac catcatgatc
    62641 aactccggcg cgtacattgc ggaaatcacg cgtggtgcgg tgctgtctat ccacaaaggt
    62701 tttcgtgaag caggactggc gctcggtctt tcacgttggg aaaccattcg ctacgtcatt
    62761 ttaccgctgg cactgcgtcg tatgctgccg ccgctgggta accagtggat catcagcatt
    62821 aaagacacct cgctgtttat tgttatcggc gtggcggaac tgacccgtca ggggcaagaa
    62881 attattgccg gtaacttccg cgcccttgag atctggagcg ccgtggcggt gttctatctg
    62941 attattacgc tggtgctgag ctttattctg cgtcgtctgg aaagaaggat gaaaatcctg
    63001 tgattgaatt taaaaacgtc tccaagcact ttggcccaac ccaggtgctg cacaatatcg
    63061 atttgaacat tgcccagggc gaagtcgtgg tgattatcgg gccgtccggt tccggtaaat
    63121 cgaccctgct gcgctgcatc aacaaactgg aagaaatcac ctccggcgat ctgattgtcg
    63181 atggcctgaa ggttaacgat ccgaaagttg acgagcgcct gattcgccag gaagcaggta
    63241 tggtgttcca gcagttttac cttttcccgc atctgacggc gctggaaaac gttatgtttg
    63301 gcccgctacg cgtgcgtggc gcgaacaaag aagaggcgga aaaactggca cgtgagctgc
    63361 tggcgaaagt cggtctggca gaacgtgcac atcactaccc ttccgaactt tctggtggtc
    63421 aacagcagcg tgtggcgatt gcccgcgcgc tggcggtgaa gccgaaaatg atgctgtttg
    63481 atgaaccgac ttccgctctt gacccggaac tgcgccatga agtgctgaag gttatgcagg
    63541 atctggctga agaagggatg acgatggtga tcgtgaccca cgaaatcggt tttgccgaga
    63601 aagtagcttc gcgcctgatc ttcattgata aaggccggat tgcggaagat ggcgatccgc
    63661 aggtgttgat caagaacccg ccgagccagc gcttgcagga atttttgcag cacgtctctt
    63721 aataagacac attgcctgat gcgctacgct tatcaggcct acaggatatc tggcaactta
    63781 ttaaaattgc atgaacttgt aggacggata aggcgttcac gccgcatccg gcaaaaaagc
    63841 ccgcacgttg tcagcaacct gcttaatatc ccttcctccc tttcacccga aagggaggca
    63901 caccagattc ctctcattta aaatcgcccc ttccccagca tctatactta tctttttgct
    63961 ctgttttctc actggaggag tcatgcggtg gatcctgttc atcctcttct gcctgctggg
    64021 cgcacctgcc cacgcggtat ccatacccgg cgttacaacc acaacgacaa cggactcaac
    64081 gactgaaccg gccccggaac cggatatcga acaaaaaaaa gcggcctatg gcgcactggc
    64141 ggatgtgctg gataatgaca cctcgcgtaa agagttgatc gaccagttgc gcaccgtcgc
    64201 cgcgacgcca cctgctgaac cggtcccgaa gatcgtgccg ccgacgctgg ttgaagagca
    64261 aaccgtgctg caaaaggtca ccgaagtcag ccgccattat ggtgaagccc tttccgcccg
    64321 cttcgggcaa ctttatcgca atatcaccgg ctccccgcat aagccgttta atccgcaaac
    64381 cttcagcaat gcgctgaccc atttttcaat gttagcggtt ttagtgtttg gtttttactg
    64441 gctgattcgc ctgtgcgcac tgccgctgta tcgcaaaatg ggtcagtggg cgcggcaaaa
    64501 aaatcgtgag cgcagtaact ggttgcagct tccggcgatg attatcgggg cgtttattat
    64561 cgacctgctg ttactggccc tgacattatt tgtcggccag gtattaagcg acaacctgaa
    64621 tgcaggcagt cgcaccatcg ctttccaaca aagtttgttt ctcaacgcct ttgccctgat
    64681 tgaatttttc aaagccgtac tacgcctgat tttttgccca aacgtggcgg aactgcgccc
    64741 gttcacgatt caggacgaga gcgcccgtta ctggagtcgt cgcctgagct ggttaagcag
    64801 tttgataggt tatggcctga ttgtggccgt gccgattatc tctaatcagg tgaatgtaca
    64861 aattggcgcg ctggcgaacg tcatcattat gctgtgcatg accgtctggg cgttgtacct
    64921 gatctttcgt aataaaaaag agattaccca gcatttgctc aactttgcgg agcattcgct
    64981 ggcctttttc agcctgttta tccgcgcctt tgcgctggtg tggcactggc tggcaagcgc
    65041 ctattttatc gtgctgtttt tcttttcgtt gttcgatccg ggcaatagcc tgaaatttat
    65101 gatgggtgca acggtgcgca gcctggcgat tattggtatc gcagcgtttg tttccggtat
    65161 gttttcccgc tggctggcaa aaaccatcac tctctcgcca catactcagc gtaactatcc
    65221 ggagttgcaa aaacggctga atggctggct ttcggcggcg ctgaaaacgg cgcgtattct
    65281 gaccgtctgc gtggcggtaa tgctgctgtt gagcgcatgg ggattgttcg atttctggaa
    65341 ctggctgcaa aacggcgcgg ggcagaaaac cgtagatatc ctgatccgta tcgcactcat
    65401 tcttttcttc tcggcggttg gctggacggt gctcgccagt ttgatcgaaa accggctggc
    65461 ttcggatatt catggccgcc cgctacccag cgcccgcacg cgtaccctgc ttacgctgtt
    65521 tcgtaacgcg ctggcggtga ttatcagtac catcaccatc atgattgtgt tgtcggaaat
    65581 cggcgtcaat atcgcgccat tgctggcagg tgccggggca ttaggtctgg ctatctcgtt
    65641 tggttcgcaa acgctggtga aagatattat caccggggta tttattcagt ttgaaaacgg
    65701 catgaacact ggagatttgg tgactattgg gccgttgacc ggcactgtgg aacggatgtc
    65761 gattcgctcc gtgggcgtac gctaggatac cggggcgtac cacatcattc cgtggtcttc
    65821 gattaccacc tttgccaact ttgtccgcgg cattggttcg gtagtggcaa actacgatgt
    65881 tgatcgccat gaagatgctg ataaagccaa tcaggcactg aaagatgcgg tagcggaatt
    65941 aatggaaaac gaagaaattc gcgggctgat tattggtgaa ccgaattttg ccgggattgt
    66001 cggcttaagc aataccgcgt ttacactgcg tgtttcgttc actacgctgc cgctcaaaca
    66061 gtggacggta cgctttgccc tcgacagcca ggtgaaaaaa catttcgacc tggcgggcgt
    66121 tcgcgcgcca gtgcagactt atcaggtgct gcctgctccg ggcgcgaccc cggctgaacc
    66181 gttaccgccg ggggagccaa cgctttaacg ctggcgattg acaaaacggc ggcgctgctc
    66241 gtcgttcata aaggtccagg caataaagcg actctgcttt tgcccctggg ccatctcttt
    66301 tttaaccacc ttcaccgcgc ccacgtccgt cagagcacga tacaacggcg gtaagttttc
    66361 accacgagat accagtgaag taaaccacat cacctgcttc gcgaagcctt tgctctcttc
    66421 aatcatcttt ttgataaagg tgacttcacc gccttcacac cacaactcct gttgctggcc
    66481 gccaaagttc agtgcatcgt ctttgttcag ccccaggtta cggcgtttac gctcactacc
    66541 tgcccgcgcc gcagcggcag aatcgtggaa tggcgggtta cacaaggtcg cgtcgtattg
    66601 ctcgttttta tggatgatgc cgttaaaaat cgccccactc tctttttgcc gacgcagacg
    66661 aatggcgcga ttaagccccg gattagcact gatgatcgcc tgcgcgctgc ttaacgcctg
    66721 gctgctggtt tcactaccgg taaaacgcca gccatattca tgtacgccaa tcagcggata
    66781 aatacagttc gcaccaacgc cgatatccag aatgctggca ttggctggaa ttgttccgct
    66841 tgcttctgcc agtaaatcgg caaggtgatg aatataatcc gcccggcccg gtactggcgg
    66901 gcagagaaaa ccgtcgggga tatcccagtt cgctacggcg taaaaatggg ccagcaacgc
    66961 cttattgagc gccttcaccg ccagcggatt ggcaaagtct acgctttgct cccctgcggg
    67021 tgtaagcgtg aggaattgcc tgagttcagg attgacctga caaagcgtgg cgagatcgta
    67081 gcggctgtga tgacggttgc gcggatgcaa ccccggtttc tgggcggaca tagcattctc
    67141 ctttaagcag cggcgtaaga tacccgcata acgcgacagg gtaaatatct ggctgcgttt
    67201 atcatttcta aaaaccttaa catggctatg gtttatggca attcatcata aaaatcagca
    67261 tcatgcatgt cattgatttg tcttattttg taaaaatata tcgttcaaaa aataagcgta
    67321 tctctgtcaa ttttttgcgt cattttcatc gcccaggcga cctacactta tcaggcacta
    67381 cccgttgtat ttatgaggat gtcattatga aaaagtgcct cacactactg attgccaccg
    67441 tcctgagcgg aatctctctc acggcttatg ccgcgcaacc gatgagtaac ctggacagcg
    67501 gtcagctacg gcccgccggt accgtttcgg caactggcgc atcaaaccta agcgatctgg
    67561 aggataaact ggcagaaaaa gcgcgcgagc aaggcgcgaa aggttatgtc attaattccg
    67621 caggcggaaa tgaccagatg ttcggtactg caaccatcta caaataaccc caccaacgta
    67681 actgcattcg cctctggatg cagttttcgc ctgttttgta atcaaaaaac atcaaatatg
    67741 aacgcaatgt aaataaatgt atttcttttt cgcgcaatgg gtgatagaaa atcgcgccaa
    67801 gtgataatgc ttatcaaaat tattatcact tgcacgaaca ctatcacgag attaacagtg
    67861 gcatcgcatc cgcagagagg ctttctcgtg gcagtgaaaa tttcaacata taagaaaaag
    67921 tcacctgcaa aatggaaaac aatcgcaatt tccctgccag acaatttcat tcgctcacgt
    67981 tctttgccgg tctttgtatt ggcatcacgc ctgtggctca ggcactcgcc gccgaagggc
    68041 aaactaacgc ggatgacacg ctggttgtcg aagcatcaac gccttcgctt tatgcgccac
    68101 aacaatctgc agatccgaaa ttctcgcgtc cggtagcgga tactacccgc acgatgacgg
    68161 tgatttctga acaagtgatt aaagatcagg gcgcaaccaa ccttaccgac gcactcaaaa
    68221 acgtccccgg cgtgggtgcg ttttttgcgg gtgagaacgg taactccacc actggcgacg
    68281 ccatttatat gcgcggtgct gatacctcta acagtattta tattgatggc attcgcgata
    68341 tcggcagcgt ctcgcgcgac accttcaata ccgagcaggt cgaagtgatt aaagggccgt
    68401 ccggcaccga ctacgggcgc agcgcaccga caggctcgat caatatgatc agcaagcagc
    68461 cgcgcaatga ttccggcatt gacgcctccg ccagtattgg tagcgcctgg ttccgccgcg
    68521 gcacgctgga cgtcaatcag gtcattggtg ataccaccgc ggcgcgcctg aatgtgatgg
    68581 gcgaaaaaac gcacgatgcc ggacgcgaca aagtcaaaaa tgagcgttac ggcgtcgccc
    68641 cttctgtcgc ttttggcctt ggtacagcga atcgtttgta tcttaattat ctgcatgtca
    68701 cccagcacaa cacgccagac ggcggcattc caaccatcgg tttgccgggc tattctgccc
    68761 catctgcggg aacggcggcc ctgaatcatt ccggaaaagt tgatacgcat aacttttacg
    68821 gcacggattc cgattacgac gattcgacca ccgacaccgc taccatgcgt tttgagcacg
    68881 acatcaacga taacaccacc attcgcaaca ctacccgttg gtcgcgcgtg aagcaggatt
    68941 acctgatgac ggcgattatg ggtggggcgt cgaatattac ccagcccacc agcgatgtga
    69001 atagctggac gtggtcacgc acggcgaata ccaaagatgt gagtaataaa attctcacca
    69061 accagaccaa cctgacctcg acgttctata ccggttctat cggtcatgat gtcagtaccg
    69121 gcgtggaatt tacccgtgaa acgcagacta actacggcgt taatccggtg acgttaccgg
    69181 cggtaaatat ttatcatcct gacagcagca ttcatcccgg cggactgacg cgcaacggcg
    69241 caaacgccaa tggtcagacg gataccttcg caatttacgc cttcgatacg ctgcaaatca
    69301 cccgtgattt tgagctgaac ggcgggatcc gtctggataa ttatcatact gaatatgaca
    69361 gtgccaccgc ctgcggcggc agcggacgcg gtgccatcac ctgcccagct ggtgtggcaa
    69421 aaggttctcc agtcaccacc gtcgacaccg ccaagtcggg caatctggtg aactggaaag
    69481 ccggggcgct gtatcacctg acggaaaacg gcaatgtcta tattaactat gccgtttccc
    69541 agcagcctcc gggcggcaac aacttcgccc ttgcgcagtc tggcagtggt aacagtgcca
    69601 accgcaccga ttttaaaccg caaaaagcca acaccagcga gattggcacc aaatggcagg
    69661 ttctggataa acgcctgttg ctcaccgccg cgctgttccg tactgatatc gaaaatgaag
    69721 ttgagcaaaa tgatgacggg acttactcgc aatacggtaa gaaacgcgtc gaaggctatg
    69781 agatatccgt ggcagggaat atcactcccg cgtggcaggt gattggcggc tatacccagc
    69841 aaaaagcaac catcaaaaac ggcaaagatg ttgcccagga tggttcctca tcgctgccgt
    69901 ataccccgga gcacgccttc accttatgga gccaatatca ggcaaccgat gatatttctg
    69961 tgggtgcggg cgcacgctat atcggcagta tgcataaagg ttcagacggc gcggtgggaa
    70021 cgccagcgtt taccgaaggt tactgggtcg ccgatgccaa actggggtat cgggttaatc
    70081 gcaatctcga cttccagcta aacgtctaca acctgtttga taccgattac gtcgcctcaa
    70141 tcaataagag cggctaccgt tatcacccgg gcgagccaag aaccttcttg ctcacagcca
    70201 atatgcattt ctgattcaga tgtggggcgc aggccccact ttttggagaa cttgtatgat
    70261 gtaccacatt cccggcgtgt tatcgccaca ggacgtcgcc cgttttcgcg aacaactgga
    70321 acaagccgaa tgggtggatg gacgcgtcac cactggcgca caaggtgcgc aagttaagaa
    70381 caatcaacag gtcgacaccc gcagcacgtt atacgccgcc ctgcaaaatg aggtgctgaa
    70441 cgcggttaac caacatgctt tgttctttgc cgcggccttg ccgcgtaccc tttccacgcc
    70501 gctgtttaat cgctatcaga acaatgaaac ctatggtttt catgtggatg gcgcagtacg
    70561 cagccatcca caaaacggct ggatgcgcac tgacctttct gccacgctgt ttttaagcga
    70621 tccacaaagc tacgacggcg gcgaactggt cgttaatgac accttcggac aacatcgggt
    70681 aaaactcccg gcaggcgatc tggtgttgta tccctccagc agcctgcatt gcgtgacacc
    70741 cgtaacccgc ggcgtacgag tggcatcatt tatgtggatc cagtcgatga tccgtgatga
    70801 taaaaagcgc gccatgctgt ttgaactgga caacaatatt cagtcgctga aaagccgcta
    70861 cggcgaaagt gaagagatcc tgtcgctgct caatctttat cataatctgc tgcgggaatg
    70921 gtcggagatc tgacgcttga actgcccgag ttcgtcccta tattagctac acttaactgt
    70981 acaagcattg atatggggag gacgatatgg catccggttg ggctaacgat gacgccgtca
    71041 acgaacagat caacagtaca attgaagatg cgattgcccg cgctcggggt gaaattccgc
    71101 gtggcgaaag cctgtatgaa tgtgaagagt gcggtgcccc catcccgcaa gcccgtcggg
    71161 aagccattcc tggcgtgcgc ttatgtattc attgtcagca ggagaaagat ttacaaaaac
    71221 cagcttatac aggatataat cgcagaggtt cgaaagacag ccagttacgt taactacaac
    71281 tggcgagatg tatagcgagt ataatttgta ttttgcgtgg cattccgtgc ctttaacgct
    71341 gctgtgactg ccacgctgca taaaacaagt ccataagtac aacaaataaa tggtttatca
    71401 gtatgttaga tatcaatcaa tttatttgaa caaggcggtc aattctcttc gattttatct
    71461 ctcgtaaaaa aacgtgatac tcatcacatc gacgaaacaa cgtcacttat acaaaaatca
    71521 cctgcgagag attaattatg aaaactatca atactgttgt tgctgctatg gctctttcaa
    71581 ctctgtcatt tggcgtgttt gccgcggaac cggtaacggc atcccaggca cagaacatga
    71641 ataaaatcgg cgtggtttct gccgatggcg catccaccct cgatgccctg gaagcgaaac
    71701 tggctgagaa agcggcggca gctggggcga gtggatacag catcacttcc gccaccaaca
    71761 acaataaatt aagcggtact gcggtaattt ataagtaatt cgtaggacgg ataaggcgtt
    71821 tacgccgcat ccggcaacag atgcctgatg cgacgctacc gcgtcttatc aggcctacaa
    71881 aatccgaacc gttggacgga tcaggcgttt acgctacgtc cagcacaagt atttccctgt
    71941 ctgtttgccg acagacgcat atgctctaac cctcattgat cctatgttac ccttgtttgc
    72001 ccgtccgcca ctggacgggc ttttttttag ctggctaact gctgacagaa agcctgcaac
    72061 gtctcttccg acataccaat ctgccgcgcc gcatcacaaa tggcctgcca gcttccccca
    72121 tccaatggaa tcccctgctc ctgccgttcg cgacgcgtgt tcacttccca ctcgcccggg
    72181 agcaaaatcg gcttatcgtc atcatgcggc gaggctttca cccactcggc aaaggcttcg
    72241 gtctgcgcgc tacaatccgg cgcgccgaac agttccggat tgatgatgat agtggtcatg
    72301 cagttaagaa tggcatcggg actggtttgt aacgtttcct gatgcgtcgt tttaccgcca
    72361 gaaagcgccc cgccaagaat ttcacacatc gctgcaaggg cgtagccttt atgttcggca
    72421 aaggtcaaca gcgaacccaa cggcgactcc tgcattaccg ccggatttgt cgtcggcacg
    72481 ccgttaacgt caatcagaca acctggcggc acggggacgc ctttatgcca ggcgacgcga
    72541 gttttgccaa atgcaatggc gctggtggcg taatcaagca acagcggaaa atcatcttta
    72601 cgtgggaaaa ccacacagaa cggattggtg ccaaagcggc tgtcgcgacc gtggaacggc
    72661 gcgaccattg gaataccgac cacgctaaca aagtggatag agacaaaccc cgccgctgca
    72721 cactgctccg cccagtaacc gatacggccg atatgatgcg agttatgtag cgccacggcg
    72781 gcaataccgt gctgatgcgc tttctcaatc cccagcgcca tcgcttcatg tgccgcgacc
    72841 tgaccaaatg cgcgatcgcc atcgagcgtg accgccgccc ccgcctcttt aacgattttg
    72901 gcatgatggt taatttgcag gtgcccctga ctccaggagc gtacatagct tgggatcatg
    72961 ccaataccat gtgaatcatg ccctgccagg tttgccgcga ttaaatgatc ggcaactaat
    73021 ttcgcttctt gttcctcgct acccatctga cgaaatacag cctgaataaa gctgtgaagc
    73081 gtctgagcat caaagcgatg accactttcc atggttaact cctgtttatg ttatgtgttt
    73141 gttgtttttt tatgctgcac gccgggcgcg agaaggtcaa taaagatgca ggaatttcat
    73201 attgcggtgg ggtaaaagcc ggatgacgtg gctcatccgg ctgagataaa aattaaaatg
    73261 cctgattaac gcgcgccagg ccatcgctga tagttgccgc ttcacccgta gccaccaggc
    73321 agcaagccat ctggattttc agcgattcgg gaatcggttc gctgccagcc aggcaacgct
    73381 caatccactg cgccgtggtt tccggatctt ttgcttgtgg caataactcg ctaccagcag
    73441 ggtcctgttt ttcatacagc acccgcatcc cttcacggtc aatgagattg atctgcgggc
    73501 agcgctgcgg attagcatac acttcacctt cagtaccatg cattagtagc gcccgcccgc
    73561 cgatatcgct aaagaacttc gcgactcgcc cgatgtattc cggatgcgaa acgctggaaa
    73621 gacgcagtgc ctcaccttcg gcaaatggcg tcgccagttt cgccagggta tgtgcactgt
    73681 tacgcacgcc catccgccag cgcatcgcca gttgtttttc cagcggcggg caaaacgcgc
    73741 cgacaggcat aaacaccggt tgatgttcgt cgagcttcgc ctgcgcctgc ccgccgtgca
    73801 acgttggcgt aatacccatc agttcgaaaa tggtttcggt cagcacgcga gttggatctt
    73861 cgctaacccc gtgaaccacc acaggaaaac cgagtttgtg caggagaatc gccaacaacg
    73921 gcgtcaggtt ggcctgttta cgcgcgccgt tgtaactggg gatgacaatc ggcatcggct
    73981 tgcctgcagg cggcgtcagc ttgatggtgt gattttgcat ggcttcgtaa aagccgagca
    74041 tctctgcttc cccttcccct ttgatacgca gcgcaatcaa tacaccgccc aactcaaggt
    74101 cagggacttc accgttgagc atatgggcat acagaccgcg cgcagtatcc cggtctaaat
    74161 cgcgcgcgtg gtttttcccg cgcccgatct ctttaatgat tttgcgatag tccatttacg
    74221 actccttacc tgacttacat cattaacgcc gccgacggcg tggggatttc gttttttctt
    74281 tctttttaac tataccttca gggacttccg gttgctctat cggaaatacc ggtaatgcat
    74341 ccagtagccg cttgccataa tttttggtca gcaagcgttt gtcgtagata accacttcgc
    74401 cccagcaacc gtgacttcga atcagtcgcc caacctgctg aatcaggtta aacgaggcgc
    74461 tcggcaagct ttgcacctca aatggatagc ggttgaggct tttcagccat tccccttcgg
    74521 tgatcaccac cgggctgtcg ataggcggaa aagcgatttt gtggatatgc acctggctga
    74581 gcatatcacc tttcaaatca agcccttcgg caaatgactg taagcccacc agcacactac
    74641 gctcaccgtt ggcgacgcgt ttgcggtgca gttcaactaa acggtaacgc ggctgatcgc
    74701 cctgcaccag caacatcaga cgtaaatccg tcacatagtc gagaaagcgc tgcatcgcac
    74761 gcccgctggc aaacagcacc aacataccga gatgtttttt gctctccacc tgctcacgga
    74821 aaaaggccgc catttcagca atatgctgtt cttcgttgtc gatggaaggc tcaacgcgca
    74881 tccggggaat aacaattttg ccctgttcgc agtggttaaa gggggaatcc agcgccacaa
    74941 aacggtcgcc cgctttctct ttcagcccgc tcatctcctg caaacgcgaa aaactgttca
    75001 gcgaacgcaa ggttgcggag gtgacaataa tgtgcggaat actgcgccac agcagccttt
    75061 ccagctgatc gctgacacgt attcccacgc agtgaaacca gaggtgtagc tgcccttcgc
    75121 gctcttcccg cgtcgcccat ttggtcaccg gtgcaccgga agattgcgcc agcgaagcca
    75181 gccgccagag tttgctttgc gcctcgaaca tccccaacgc gcggttcatc tgcaaaatca
    75241 accgatgcag acgtacaatg tcatggctgc cggttttctc acttaaatcg ttaagaaata
    75301 actccgccag accacgcagc atctcggtga gttttgccag ccgctggcag atctccagca
    75361 cttcatcagg cagttcgccc atcgcaaaac ggtgttctgc ttcctgccca gcaggcatgt
    75421 agagattgag aatgttgttt aatgaggcta taagctcata cagctcttca caatgcgcat
    75481 tcaaacgttc agggatcgcc agcggcggga tggtcttcgg gcgaaactgc tccatgcagg
    75541 tagcgaccag tttcgtgaac aagtccagct gtagccgata ccatggcgcg gtgatttcgg
    75601 cactcatctc cagcgcatcc cgcgccacat ctggcaggtg atggccttcg tccagcacca
    75661 gcagtaaatt tttcgggtca ggcaataccg cttcgctttc catcgccgcc atcaccagcg
    75721 catggtttgc caccaccact tccgcttcct gaatctcccg acgagcaaca aaaaacgggc
    75781 attcgcgata gtagtaacag ttgcggttga ggcagctggc tttgtcggta cttaaacgac
    75841 gccagagatc gtcatctata gcgatatccg tatgatcgcg cagaccatcc catttataag
    75901 tgtcgagatc gcccttcagc ttcgcacaac gtttttgctc ttcctgatta tttggcgtca
    75961 gttcgtcgtc aagaaacgcc agcagatcct gttgcgtggg ctcagtgctg gcaagcgccg
    76021 tcagattacg cgggcaaacg tagcgcccac gcccaaaagc ggcagtgaat ttaagatcgg
    76081 gaatgatctt tttcagcaac ggtaaatctt tgctgtagat ctgatcctgc aatgccacgt
    76141 tggcggtgct caccaccagc gttttttgct cttcgcgggc aatggcgatg ccgggaatca
    76201 aataggagag cgttttccca acgccagtgg gggcttcaat cgccagatgc cgcccttctt
    76261 ctccggccag cgttttggcg acgtccgcga tcatctgccg ctgcggcgca cggggaataa
    76321 agtcggggat ctgttcctga agcgccttat accaggcggc aatttgcgct ttaagcgcgg
    76381 cggttaatgc catgagaaaa cctgaaatac tgtataaaca gccaatattg tggcattttt
    76441 cggttttcag gggtaatggt ttttctgttt ctggaaagcg gctcgcaaac acgccctctc
    76501 gcaatatatt tgattgaaaa ttcgacggca cagtgagcgc cagtcttcag ggcagaaaac
    76561 agcaagtgat accgcatttc gtggttacac ttgccgccgt catcgcttcg caggaaccgg
    76621 tattgccgac cctggcctga gccagggcca tcaatgccat tattctgctg cgatctttac
    76681 agccatgcgt ctggcgtcca ctcggataaa actttagaaa aacgatattg tgcatagcgg
    76741 accatatcac gatataacga acttatgcgc aactggttgg cctcttcatt agacagatta
    76801 ttgaaatcaa gcaataccgt tttgtatgta ttaaaagttt gattgcttat catactttca
    76861 tctaaagaat gagctttcgc caataaagca aatgcataac gttttaaagt ttttgacgca
    76921 tcaggaatac cgttaaagac attactgtga gaaatatttg caaatacggc agacaggcag
    76981 aaaaatattt tagacttatc actttcactg gcctcgttga gatccagcat cgttaataga
    77041 ctagcgtaat gtttatcttt cagagtatgc tgttgccatt gatctgaatc gttcattaaa
    77101 gaggaaaatg atgaattaag ttctgttggt tgattattgc attcgggcac aaaatctgtt
    77161 tttcgcatta ttgctgacat aaatatatca cgaatataac ctttgaatcc aagagcattg
    77221 agaatcaatt ggtgtttctt atttctctgc gcatattccc atccttttgc aaataaagga
    77281 aatgcacgcg tacaaatcgt ttctggatca tctctgacaa cttcatgggt atcctgcaaa
    77341 gatggataaa tagagtaatt attccagata gtctttttct taacctctgg ctcaagcatt
    77401 gagattaacg cctggtgcga taa
//
LOCUS       DAZFKF010000011        76478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFKF010000011 DAZFKF010000000
VERSION     DAZFKF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.011"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(65..997)
                     /gene="glsA"
                     /locus_tag="ACHKA1_000910"
     CDS             complement(65..997)
                     /gene="glsA"
                     /locus_tag="ACHKA1_000910"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO5678607.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            1259..3763
                     /gene="copA"
                     /locus_tag="ACHKA1_000911"
     CDS             1259..3763
                     /gene="copA"
                     /locus_tag="ACHKA1_000911"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO5678608.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADSDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITMMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(3978..4319)
                     /gene="ybaQ"
                     /locus_tag="ACHKA1_000912"
     CDS             complement(3978..4319)
                     /gene="ybaQ"
                     /locus_tag="ACHKA1_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO5678609.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLALKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            4457..5251
                     /gene="ybaP"
                     /locus_tag="ACHKA1_000913"
     CDS             4457..5251
                     /gene="ybaP"
                     /locus_tag="ACHKA1_000913"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO5678610.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTNDTPFANLPACEALEERISEEQLQKLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            5455..5934
                     /gene="ybaK"
                     /locus_tag="ACHKA1_000914"
     CDS             5455..5934
                     /gene="ybaK"
                     /locus_tag="ACHKA1_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO5678611.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(5971..7623)
                     /gene="ushA"
                     /locus_tag="ACHKA1_000915"
     CDS             complement(5971..7623)
                     /gene="ushA"
                     /locus_tag="ACHKA1_000915"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO5678612.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARVLPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QLDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            7841..9061
                     /gene="fsr"
                     /locus_tag="ACHKA1_000916"
     CDS             7841..9061
                     /gene="fsr"
                     /locus_tag="ACHKA1_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO5678613.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            9299..10975
                     /gene="ybaL"
                     /locus_tag="ACHKA1_000917"
     CDS             9299..10975
                     /gene="ybaL"
                     /locus_tag="ACHKA1_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO5678614.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(11105..12409)
                     /gene="gsk"
                     /locus_tag="ACHKA1_000918"
     CDS             complement(11105..12409)
                     /gene="gsk"
                     /locus_tag="ACHKA1_000918"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO5678615.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            12561..13520
                     /gene="aes"
                     /locus_tag="ACHKA1_000919"
     CDS             12561..13520
                     /gene="aes"
                     /locus_tag="ACHKA1_000919"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO5678616.1"
                     /translation="MKPENKLPVLDLISAEMKIVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNYADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            complement(13517..14479)
                     /gene="hemH"
                     /locus_tag="ACHKA1_000920"
     CDS             complement(13517..14479)
                     /gene="hemH"
                     /locus_tag="ACHKA1_000920"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO5678617.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(14611..15255)
                     /gene="adk"
                     /locus_tag="ACHKA1_000921"
     CDS             complement(14611..15255)
                     /gene="adk"
                     /locus_tag="ACHKA1_000921"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO5678618.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            complement(15436..17310)
                     /gene="htpG"
                     /locus_tag="ACHKA1_000922"
     CDS             complement(15436..17310)
                     /gene="htpG"
                     /locus_tag="ACHKA1_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO5678619.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(17420..18025)
                     /gene="recR"
                     /locus_tag="ACHKA1_000923"
     CDS             complement(17420..18025)
                     /gene="recR"
                     /locus_tag="ACHKA1_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO5678620.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(18025..18354)
                     /gene="ybaB"
                     /locus_tag="ACHKA1_000924"
     CDS             complement(18025..18354)
                     /gene="ybaB"
                     /locus_tag="ACHKA1_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO5678621.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(18407..20338)
                     /gene="dnaX"
                     /locus_tag="ACHKA1_000925"
     CDS             complement(18407..20338)
                     /gene="dnaX"
                     /locus_tag="ACHKA1_000925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO5678622.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    complement(19010..19074)
                     /gene="dnaX"
                     /locus_tag="ACHKA1_000925"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(20467..21018)
                     /gene="apt"
                     /locus_tag="ACHKA1_000926"
     CDS             complement(20467..21018)
                     /gene="apt"
                     /locus_tag="ACHKA1_000926"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO5678623.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(21171..21548)
                     /gene="ybaN"
                     /locus_tag="ACHKA1_000927"
     CDS             complement(21171..21548)
                     /gene="ybaN"
                     /locus_tag="ACHKA1_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO5678624.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            21618..22145
                     /gene="priC"
                     /locus_tag="ACHKA1_000928"
     CDS             21618..22145
                     /gene="priC"
                     /locus_tag="ACHKA1_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO5678625.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            22159..22320
                     /gene="rsmS"
                     /locus_tag="ACHKA1_000929"
     CDS             22159..22320
                     /gene="rsmS"
                     /locus_tag="ACHKA1_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO5678626.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(22373..22471)
                     /locus_tag="ACHKA1_000930"
                     /pseudo
     CDS             complement(22373..22471)
                     /locus_tag="ACHKA1_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22532..25894)
                     /gene="mscK"
                     /locus_tag="ACHKA1_000931"
     CDS             complement(22532..25894)
                     /gene="mscK"
                     /locus_tag="ACHKA1_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO5678627.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKIKVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(26022..26669)
                     /gene="acrR"
                     /locus_tag="ACHKA1_000932"
     CDS             complement(26022..26669)
                     /gene="acrR"
                     /locus_tag="ACHKA1_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO5678628.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            26811..28004
                     /gene="acrA"
                     /locus_tag="ACHKA1_000933"
     CDS             26811..28004
                     /gene="acrA"
                     /locus_tag="ACHKA1_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO5678629.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            28027..31176
                     /gene="acrB"
                     /locus_tag="ACHKA1_000934"
     CDS             28027..31176
                     /gene="acrB"
                     /locus_tag="ACHKA1_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO5678630.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            31722..32096
                     /gene="tomB"
                     /locus_tag="ACHKA1_000935"
     CDS             31722..32096
                     /gene="tomB"
                     /locus_tag="ACHKA1_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO5678631.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            32122..32340
                     /gene="hha"
                     /locus_tag="ACHKA1_000936"
     CDS             32122..32340
                     /gene="hha"
                     /locus_tag="ACHKA1_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO5678632.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            32512..33063
                     /gene="maa"
                     /locus_tag="ACHKA1_000937"
     CDS             32512..33063
                     /gene="maa"
                     /locus_tag="ACHKA1_000937"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO5678633.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            33179..33649
                     /gene="ylaC"
                     /locus_tag="ACHKA1_000938"
     CDS             33179..33649
                     /gene="ylaC"
                     /locus_tag="ACHKA1_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO5678634.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            33813..35363
                     /gene="pdeB"
                     /locus_tag="ACHKA1_000939"
     CDS             33813..35363
                     /gene="pdeB"
                     /locus_tag="ACHKA1_000939"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO5678635.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGADCSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(35405..35758)
                     /gene="ybaA"
                     /locus_tag="ACHKA1_000940"
     CDS             complement(35405..35758)
                     /gene="ybaA"
                     /locus_tag="ACHKA1_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO5678636.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(35878..35974)
                     /gene="ffs"
                     /locus_tag="ACHKA1_000941"
     ncRNA           complement(35878..35974)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKA1_000941"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            36137..36448
                     /locus_tag="ACHKA1_000942"
     CDS             36137..36448
                     /locus_tag="ACHKA1_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO5678637.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(36479..37051)
                     /gene="ybaY"
                     /locus_tag="ACHKA1_000943"
     CDS             complement(36479..37051)
                     /gene="ybaY"
                     /locus_tag="ACHKA1_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO5678638.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            37269..38129
                     /gene="tesB"
                     /locus_tag="ACHKA1_000944"
     CDS             37269..38129
                     /gene="tesB"
                     /locus_tag="ACHKA1_000944"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO5678639.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKKMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(38178..39464)
                     /gene="amtB"
                     /locus_tag="ACHKA1_000945"
     CDS             complement(38178..39464)
                     /gene="amtB"
                     /locus_tag="ACHKA1_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO5678640.1"
                     /translation="MKIATIKTGLASLAMLPGLVIAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(39494..39832)
                     /gene="glnK"
                     /locus_tag="ACHKA1_000946"
     CDS             complement(39494..39832)
                     /gene="glnK"
                     /locus_tag="ACHKA1_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO5678641.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(40013..41794)
                     /gene="mdlB"
                     /locus_tag="ACHKA1_000947"
     CDS             complement(40013..41794)
                     /gene="mdlB"
                     /locus_tag="ACHKA1_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO5678642.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(41787..43559)
                     /gene="mdlA"
                     /locus_tag="ACHKA1_000948"
     CDS             complement(41787..43559)
                     /gene="mdlA"
                     /locus_tag="ACHKA1_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO5678643.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(43589..44047)
                     /gene="decR"
                     /locus_tag="ACHKA1_000949"
     CDS             complement(43589..44047)
                     /gene="decR"
                     /locus_tag="ACHKA1_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO5678644.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(44201..45019)
                     /gene="cof"
                     /locus_tag="ACHKA1_000950"
     CDS             complement(44201..45019)
                     /gene="cof"
                     /locus_tag="ACHKA1_000950"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO5678645.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            45119..46819
                     /gene="ybaE"
                     /locus_tag="ACHKA1_000951"
     CDS             45119..46819
                     /gene="ybaE"
                     /locus_tag="ACHKA1_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO5678646.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            46884..47579
                     /gene="queC"
                     /locus_tag="ACHKA1_000952"
     CDS             46884..47579
                     /gene="queC"
                     /locus_tag="ACHKA1_000952"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO5678647.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLTDKP
                     TVMAAMKQKTGLK"
     gene            complement(47631..48029)
                     /gene="fadM"
                     /locus_tag="ACHKA1_000953"
     CDS             complement(47631..48029)
                     /gene="fadM"
                     /locus_tag="ACHKA1_000953"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO5678648.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(48135..48506)
                     /gene="ybaV"
                     /locus_tag="ACHKA1_000954"
     CDS             complement(48135..48506)
                     /gene="ybaV"
                     /locus_tag="ACHKA1_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO5678649.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(48657..50528)
                     /gene="ppiD"
                     /locus_tag="ACHKA1_000955"
     CDS             complement(48657..50528)
                     /gene="ppiD"
                     /locus_tag="ACHKA1_000955"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO5678650.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDKVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(50720..51010)
                     /gene="hupB"
                     /locus_tag="ACHKA1_000956"
     CDS             complement(50720..51010)
                     /gene="hupB"
                     /locus_tag="ACHKA1_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO5678651.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(51201..53555)
                     /gene="lon"
                     /locus_tag="ACHKA1_000957"
     CDS             complement(51201..53555)
                     /gene="lon"
                     /locus_tag="ACHKA1_000957"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO5678652.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(53743..55017)
                     /gene="clpX"
                     /locus_tag="ACHKA1_000958"
     CDS             complement(53743..55017)
                     /gene="clpX"
                     /locus_tag="ACHKA1_000958"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO5678653.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(55143..55766)
                     /gene="clpP"
                     /locus_tag="ACHKA1_000959"
     CDS             complement(55143..55766)
                     /gene="clpP"
                     /locus_tag="ACHKA1_000959"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO5678654.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGKSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(56012..57310)
                     /gene="tig"
                     /locus_tag="ACHKA1_000960"
     CDS             complement(56012..57310)
                     /gene="tig"
                     /locus_tag="ACHKA1_000960"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO5678655.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFSIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(57654..57971)
                     /gene="bolA"
                     /locus_tag="ACHKA1_000961"
     CDS             complement(57654..57971)
                     /gene="bolA"
                     /locus_tag="ACHKA1_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO5678656.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            58276..58854
                     /gene="yajG"
                     /locus_tag="ACHKA1_000962"
     CDS             58276..58854
                     /gene="yajG"
                     /locus_tag="ACHKA1_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5678657.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            58898..60373
                     /gene="ampG"
                     /locus_tag="ACHKA1_000963"
     CDS             58898..60373
                     /gene="ampG"
                     /locus_tag="ACHKA1_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO5678658.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            60832..61779
                     /gene="cyoA"
                     /locus_tag="ACHKA1_000964"
     CDS             60832..61779
                     /gene="cyoA"
                     /locus_tag="ACHKA1_000964"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO5678659.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            61801..63792
                     /gene="cyoB"
                     /locus_tag="ACHKA1_000965"
     CDS             61801..63792
                     /gene="cyoB"
                     /locus_tag="ACHKA1_000965"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO5678660.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            63782..64396
                     /gene="cyoC"
                     /locus_tag="ACHKA1_000966"
     CDS             63782..64396
                     /gene="cyoC"
                     /locus_tag="ACHKA1_000966"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO5678661.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMAPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            64396..64725
                     /gene="cyoD"
                     /locus_tag="ACHKA1_000967"
     CDS             64396..64725
                     /gene="cyoD"
                     /locus_tag="ACHKA1_000967"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO5678662.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            64737..65627
                     /gene="cyoE"
                     /locus_tag="ACHKA1_000968"
     CDS             64737..65627
                     /gene="cyoE"
                     /locus_tag="ACHKA1_000968"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO5678663.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            65776..67140
                     /gene="yajR"
                     /locus_tag="ACHKA1_000969"
     CDS             65776..67140
                     /gene="yajR"
                     /locus_tag="ACHKA1_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5678664.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCIGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(67268..67759)
                     /gene="yajQ"
                     /locus_tag="ACHKA1_000970"
     CDS             complement(67268..67759)
                     /gene="yajQ"
                     /locus_tag="ACHKA1_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO5678665.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            67927..68838
                     /gene="panE"
                     /locus_tag="ACHKA1_000971"
     CDS             67927..68838
                     /gene="panE"
                     /locus_tag="ACHKA1_000971"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO5678666.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            68801..69391
                     /gene="yajL"
                     /locus_tag="ACHKA1_000972"
     CDS             68801..69391
                     /gene="yajL"
                     /locus_tag="ACHKA1_000972"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO5678667.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(69445..70893)
                     /gene="thiI"
                     /locus_tag="ACHKA1_000973"
     CDS             complement(69445..70893)
                     /gene="thiI"
                     /locus_tag="ACHKA1_000973"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO5678668.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGVDVVSLPFYKLSTKFGDLDQSK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            71099..71341
                     /gene="xseB"
                     /locus_tag="ACHKA1_000974"
     CDS             71099..71341
                     /gene="xseB"
                     /locus_tag="ACHKA1_000974"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO5678669.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            71341..72240
                     /gene="ispA"
                     /locus_tag="ACHKA1_000975"
     CDS             71341..72240
                     /gene="ispA"
                     /locus_tag="ACHKA1_000975"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO5678670.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            72265..74127
                     /gene="dxs"
                     /locus_tag="ACHKA1_000976"
     CDS             72265..74127
                     /gene="dxs"
                     /locus_tag="ACHKA1_000976"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO5678671.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRLMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            74182..75156
                     /gene="yajO"
                     /locus_tag="ACHKA1_000977"
     CDS             74182..75156
                     /gene="yajO"
                     /locus_tag="ACHKA1_000977"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO5678672.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFSRREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEDLGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            75387..>76478
                     /gene="espY3"
                     /locus_tag="ACHKA1_000978"
     CDS             75387..>76478
                     /gene="espY3"
                     /locus_tag="ACHKA1_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspY3"
                     /protein_id="HFO5678673.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNKIRDFF
                     LDSDKQKAFKSIHKYINTLSVLNYNSALTPDPNFNIDATSDLDSYLKLDFDRLSPKQK
                     QTTLCCFWNKIASSLPEPYNSTIKHNIIFYKDGENLMIRGTISIVNEVVKTYSLPIEK
                     DDNGYYDFSGLYLAHSNISGKDPNKDPDIDFGIDMGNCNCSNVNFEHTYFYGVKFTNA
                     NCTNANFNNCRFKKCDLTNMNCTGAILDNAMIYGKEKEPEMQYPEADQIIQRITYQKS
                     DGNETKGMILTNCSCVKTTFNWADLSESDCQNVDFSEANLSNTILPDIVRMKGTKLYR
                     TDLFNPILKTEAESTEEKDISPLAKIILDY"
BASE COUNT        17942 a        20077 c        19420 g        19039 t
ORIGIN      
        1 atggaaacga cgttccatag accgaggggt ttgttaccgt tattaccttc cgtgttcatc
       61 atgatcagcc cttaaacacg ttatagccga gttgcttagc gaccgatgcc accatttttt
      121 gaccgcgaac actgttgcct tcttcgtcca gcggtggtga gaacgcggca attcccatca
      181 ctccagggac gaccgccaga ataccgccac ctacaccgct tttgcccggt aaaccaacac
      241 gatacgccca gtcaccggag cgaccataca gcccttccat catcatttcg gccagaatgt
      301 acggcacgtt gtcggcctga agaacgcgtt tatgcgtcaa cggattcaca ccacccgccg
      361 ccagcgtcgc gccaagcgtt gccagttcaa cagtattgat gagcgtggag cactgacggg
      421 tgtacacgtc acatgcttcc attgcatcac aatagagata tccggcggag tacagcagcc
      481 aggcaatggc ccggttatgg aagttggttg tttgttccga ctggttgact tcgtcagaga
      541 gcgctaccag ctcaccagcc agttgctgtt ggatatgtaa aattcgctgc cagcgttgtt
      601 cggcattttc agcgttaatc aggctggtgg tggcaatagc gccagcattt accagtggcg
      661 acagcggttt gccgccatgc aactctaagg cgataactga gttaaagggc aatccggtcg
      721 ggtcagcgcc aattttgtcc tgtaccgcct gcgggccgac atcttctaac gcaagggcta
      781 acgtacagac tttcgagatg gattccagcg caaagcggta atcactgtca cccgcactat
      841 agacgttgcc atcgctggtc acgatagcca ctgctgccag ttgaccaggc acattcgcca
      901 gaaagggaat gtaatcggca ttttgtccgc cgttaagtga gtgaaattgg gtgtaagcct
      961 gatccactgc ctgctgtaat ttgtttgcat ctaacatctt ttgttaactc ctttttatag
     1021 atgcgggagg taattcctca ccccggtgcc gattttcagg catcctgatt taacttagca
     1081 cccgcaactt aactacagga aaacaaagag ataaatgtct aatcctgatg caaatcgagc
     1141 cgatttttta atctttacgg acttttactc gcctgggtta ttaatttctt gaccttcccc
     1201 ttgctggaag gtttaacctt tatcacagcc agtcaaaact gtcttaaagg agtgttttat
     1261 gtcacaaact atcgacctga ccctggacgg cctgtcctgc ggtcactgcg ttaaacgcgt
     1321 gaaagaaagt ctcgaacagc gcccggatgt ggaacaagcg gatgtgtcta tcactgaagc
     1381 gcacgttacc gggactgcca gtgcagaaca gctgatcgaa accatcaaac aagcgggtta
     1441 tgacgcatct gtaagccacc caaaggctaa accgctggcg gagtcatcaa tcccgtcgga
     1501 agcactgaca gcggtttctg aggcgcttcc ggcagcgacc gccgatgacg atgacagcca
     1561 gcagttgctg ctgagcggca tgagctgcgc cagctgtgtc acccgcgtac aaaatgcgtt
     1621 gcaaagcgta ccgggcgtca ctcaggcacg ggtaaacctg gcggagcgta ctgcgctggt
     1681 gatgggcagt gcctccccac aagatttagt gcaggcggtg gaaaaagcgg gctacggtgc
     1741 ggaagcgatt gaagatgacg cgaaacgccg cgagcgccag caagaaaccg ccgtcgctac
     1801 gatgaagcgc ttccgctggc aggcaattgt tgcactggct gtgggtatcc cggtaatggt
     1861 ctgggggatg atcggcgata acatgatggt caccgctgac aaccgcagct tgtggttagt
     1921 tatcggcctg ataaccctgg cagtgatggt tttcgccggc ggccattttt accgcagtgc
     1981 atggaaaagc ctgctgaacg gtgcagcgac gatggatacg ctggtggcgc tgggtactgg
     2041 cgtggcgtgg ctctattcga tgagcgtcaa cctgtggccg cagtggttcc cgatggaagc
     2101 gcgacatctt tattacgaag ccagcgcgat gattatcggt ctgatcaatc tcggccatat
     2161 gctggaagca cgcgcacgtc agcgttcttc aaaggcgctg gagaaattac tcgatttaac
     2221 tccgccgacg gcgcgcctgg tcaccgacga aggtgaaaaa agcgtgccgc tggcggaagt
     2281 tcagccaggc atgctactgc gcctgacgac cggcgaccgc gtgccggtag atggcgagat
     2341 tacccagggc gaagcgtggc tggatgaagc gatgctgacg ggcgaaccga tcccgcagca
     2401 aaaaggcgaa ggcgatagcg tccatgccgg gacagtggta caggacggta gcgttctgtt
     2461 tcgcgccagt gcggttggca gccataccac gctatcaagg attattcgta tggtgcgcca
     2521 ggcccagagc agcaagccag aaatcggtca gctggcggat aaaatctcag ccgtatttgt
     2581 gccggtagta gtggttattg cgcttgtcag tgcggcaatc tggtatttct ttggtccggc
     2641 accgcagatt gtctataccc tggtgattgc caccacggtg ctgattattg cctgtccgtg
     2701 tgcgctgggg ctggcgacgc cgatgtcgat tatttccggc gtcgggcggg cggctgagtt
     2761 tggcgtgctg gtgcgggacg ccgacgcgct gcaacgcgcc agtacactcg ataccgtcgt
     2821 gttcgataaa accgggacgc tgaccgaagg gaagccgcag gttgtcgcag tgaaaacatt
     2881 tgctgattct gacgaagcgc aggcgctgcg tctggcggcg gcactggaac aaggttccag
     2941 ccatccgctg gcacgggcga tcctcgataa agcaagcgat atgcagctgc cgcaggtcaa
     3001 cggtttccgc acattgcgcg ggctgggtgt gagcggtgaa gcagaaggtc atgcgttatt
     3061 gctgggtaat caggcgttgt taaatgatca gcaagtcgat actaaagcga ttgaagccga
     3121 tatttcagct caggcatcgc aaggagcaac gcctgtgctg ctggcgattg acgggaaagc
     3181 agtagccctg ctggctgtac gcgatccatt gcgtagtgat agcgtggcgg cgctgcaacg
     3241 cctgcataaa gcgggatatc gtctggtgat gttgaccggg gataacccaa ccaccgccaa
     3301 tgcgatcgcc aaagaagccg ggattgatga ggtgatagcc ggggtgctgc cggacggtaa
     3361 agccgaagcg atcaaacgtc tgcaaagtga aggacgtcag gtggcaatgg tgggcgacgg
     3421 cattaacgac gcgccagcgc tggcccaggc ggatgtcggc attgcgatgg ggggcggcag
     3481 tgatgttgcc attgaaaccg cggcgattac catgatgcgc catagcctga tgggcgttgc
     3541 ggatgcgctc gctatttccc gcgcaacgct gcgcaacatg aaacagaacc tgctcggcgc
     3601 gtttatctac aacagtatcg gtattccggt cgccgccggt attttgtggc cgtttactgg
     3661 aacactgctt aacccggtag ttgccggagc ggcaatggcg ctctcgtcga ttaccgtagt
     3721 gagtaacgcc aaccggttgc tgcggtttaa accgaaggaa taagtacgtc attgcggatg
     3781 cgctgcttta gggcggcgca tttggcatcg acgcatgact gccggatgcg gcgtgaacgc
     3841 cttatccggc ctacggttca ggcacaggtt tgtaggcctg ataagacgcg taagcgtcgc
     3901 atcaggcaat ggtgctcgga tgccggatgc ggcgtaaacg ccttatccgg cctacgagat
     3961 gaagcgatgt tttggcctta cgcgaccttt tttgcacgct cttcacggcg tgccaaatat
     4021 tcagccactg tttcaatccg tcccaattct tcctgggtac gcatgttgtt ttcaacttcc
     4081 caaagatcaa ctgccgcctg gaggtttagc caaaaatcga ctgtggtatc aaaaactttc
     4141 gccagacgga atgccatttc agtggtgagt ttacgattat tattgatcag tgtactgacg
     4201 ctattacgat gaacatgcag caactctgct aactcattga ttttcaaagc gagcggttcc
     4261 agatattcat agagaagaat atctccgggt gtcgtcggtt ttcttgttgc ctgtttcata
     4321 gtttttaact gttgcttccc tgtgaatagc gatacaagaa catactgaat catacaaaca
     4381 cattaaacaa gtccccaact ccatttgcgc catccctcac cgcgcgctag catgatattt
     4441 cacaaaggga ggtcgtatgg atctgttgta ccgggtaaaa acgctttggg ccgcgctgcg
     4501 cggtaatcat tacacctggc ctgccatcga tatcaccctt cccggcaatc gccattttca
     4561 tctgattggc agtattcata tgggtagcca cgatatggct cccctgccca cccgtttgct
     4621 caaaaagctc aaaaacgccg atgcgctgat cgtcgaggcg gatgtttcca ccaacgatac
     4681 gccttttgct aatttgcctg cctgcgaggc gctggaagag cgcattagcg aggagcaact
     4741 ccaaaaactg cagcacatta gccaggagat gggcatttct ccctcactct tttctaccca
     4801 accgctgtgg caaatcgcga tggttcttca ggcgacgcag gcgcaaaaac tggggctgcg
     4861 ggcagaatac ggtatcgatt accagctatt gcaggcggcg aagcaacaac ataaacccgt
     4921 gattgaactg gaaggggctg aaaaccagat tgccatgttg ctccagctcc ctgacaaagg
     4981 actggcactg ctggacgata cgctgaccca ctggcacacc aatgcacggt tgctgcaaca
     5041 aatgatgagc tggtggctga atgcgccgcc gcaaaataat gaaataacgc tgcccaatac
     5101 gttcagtcag tcgctgtacg atgtgctgat gcatcagcga aatctcgcct ggcgggataa
     5161 attacgcgcc atgccgccgg ggcgatatgt ggtcgcggtc ggtgcactac acctgtatgg
     5221 agaagggaat ttaccgcaaa tgttgcgcta aaaaaatggc caatatcgct attggcccgt
     5281 caaagaggaa tttcattttt ttattattat gccgtcactt taagcgacgg tgtaactcga
     5341 ttgtcgctca atctcaccat cctgccaata ctcttaggca agatgactct tttttttgga
     5401 ccgccatcag gcgtatgctt gcttcgtttt tttgtttaag gcaggaaaat taccatgaca
     5461 cccgcagtta aattactcga aaaaaacaag atttcgtttc aaatccatac ctacgagcac
     5521 gatccggctg aaaccaattt tggcgatgaa gtcgtcaaaa aattaggttt gaatccggat
     5581 caggtctaca aaacgctgct ggtggcagtg aacggtgata tgaaacacct tgccgtggcc
     5641 gttacgccgg tcgcaggtca actggacctt aaaaaagtag caaaagcgct gggtgccaag
     5701 aaagttgaga tggccgatcc gatggtcgcg cagcgttcga cgggatacct ggttgggggg
     5761 attagcccac tggggcagaa aaaacgtctg ccaacgatta tcgacgcccc cgcacaagaa
     5821 tttgccacta tttatgtttc cggcggcaag cgcggactgg atatcgaact ggcggcaggc
     5881 gatctggcga agatcctcga tgccaaattt gctgatatcg cccgccgcga ttaagcattg
     5941 tgccggatgc gaacatccgg cactttcgga ttactgccag ctcacctcac ctttcggttc
     6001 ataaacactc acatccagcg gcgagctttt ctggatatac gctttcagca cttcggcatc
     6061 aataaagccg gtattcacat agcccggttt gttatcaagg cgcggatatc catcaccgcc
     6121 ggtggcattg aagtttaatg tcgccatacg gtaagttttc gctggatcga ccggttcgcc
     6181 tttgatttta aggtcgttca gtttgccgtc tttcgccaca aagctaacgt tggcaaattg
     6241 cgggtaggca cctgaatctg gcttcatctg cgcgacggcg gtcaggtaat caatcacctc
     6301 tttaccggtc atgtcggcat acaccaccac attgccgaat ggctgcactt tcagcacgtt
     6361 tttatagctg atatcgcctg cttcgataga atcacgaatt ccgcctccgc tcatcaccgc
     6421 aaagtcggca ccagtgcgat ccaattgtgc tgccagaatt aaccgcccca tattggtctg
     6481 tacaaaacgc actttgtcac gatcgccttc cagacgacca ttggtttcgc ctattttcac
     6541 ttccagctgc gctttgcctt tgttctggaa cggcgataac agcgagatca tttgctggtt
     6601 ttcagcgatt tcaggggtgt acagcacgcg ctcgcttttc ccgtcttccc aggtcacttt
     6661 cttcttcagg ttcaccggaa tcagctggta gttaaccatt ttcatttcgc cattacgaaa
     6721 ctcaaaatca gcccgtccca cgtatttgcc ccactcatgt gcctgcacaa tccagatgcc
     6781 gttttgttga tccggtttgc atggcgtacc cggcacgtaa tcgacctgtt ttttgttttc
     6841 tgccgccatg cagaccggat cttgcgagtg accaccgacg atcatcgcca gcgatccggc
     6901 aggcagcacg cgtgccatct ccacatcgcc cggtgcatta gagccgtgct caccattgtc
     6961 gtaatgcccc atatgggtcg ccgcgataat aatgtctggc ttttctgtct gttgcagctc
     7021 ctgaatcacc agcttcgctt catcggcggg cttacggaac tcaatatcgg tgaagttttc
     7081 tgggttgcct aacttcgctg tgtcatccgt cgtcagacca ataacggcaa ttttcagatc
     7141 ctgacgctta aacagcgccc atggtttaaa caggcgctcg ccggtacttt tctggtagat
     7201 attcgcggaa agcaacggga acttggccca cttttcctgc tgacgtaata cggtgagcgg
     7261 attatcaaat tcatgattac cgatcgccat cgcgtcatag cccaccagat tcataccgcg
     7321 aaaatcaggt tcggcatcct gtaagtcaga ctcgggcacg ccagtgttaa tgtcgccacc
     7381 ggaaagtagc agcacgctac cgccttcagc cgcaacctct ttgcggatac catccaccag
     7441 cgttttttgc gccgccagac catattcgcc atattcattg cgccaaaaat gcccatgatg
     7501 atcattggta tgcagaactg taattttgta ggttttatcc tgctcatacg ccagagcagc
     7561 ttcactcgcc agtgtaaatg tggttaacag tgctaacgcc acgccccgct gcaataattt
     7621 catacttctc tccctgacct gatttcaacg tgttgaaaaa tagtatgacg cgacatcata
     7681 gacaagtctg cgtccggtga agataaatct gcatccagat tagaaattct gtattcagcg
     7741 ttgagacttc cttcaggcgg cgcatacagg tatgttagtc gggtaataat cacaaataag
     7801 atcattcttt acatcgcaac cctgaaaaag tgactttcct atggcaatga gtgaacaaac
     7861 ccagcctgtg gcgggcgcgg ctgcgtcaac gaccaaggcc cgaacatcgt ttggtatttt
     7921 aggtgctatc agcctctcac atctgctgaa cgacatgatc caatcgctga ttctggcgat
     7981 ttatccgctg cttcagtcag agttttctct gacatttatg cagattggca tgataaccct
     8041 caccttccag ctcgcctctt cgctactgca accagtggtc ggctactgga ccgataaata
     8101 tccgatgccg tggtcgttgc caattggcat gtgctttacc ttaagtggtc tggtgctgct
     8161 tgcgctggcg ggcagttttg gcgcagttct gctggcggcg gcgctggtcg gtaccggttc
     8221 atcggtcttt catccggaat cttctcgcgt ggcccgtatg gcttccggcg ggcggcatgg
     8281 cctggcacaa tctatctttc aggtcggcgg caactttggc agttccctgg gacccttgct
     8341 ggcggcggtg attatcgcgc cttatggcaa aggcaacgtt gcctggtttg tgcttgcggc
     8401 actgctggcg atcgtggtgt tggcgcaaat cagccgttgg tactcggcac agcaccgaat
     8461 gaataaagga aaacccaaag cgacgattat caatccactg ccgcgcaaca aagtggtact
     8521 ggcggtcagc attctgttaa tcctcatttt ctcgaaatat ttctatatgg cgagcatcag
     8581 cagctattac accttttatc tgatgcaaaa attcggatta tctatccaga atgcccagct
     8641 tcatctgttt gccttcctgt ttgccgttgc ggcaggtacg gtgatcggcg ggcctgtagg
     8701 ggataaaatt gggcggaaat atgtgatttg gggctctatc ctcggcgttg cgccgtttac
     8761 gctgatttta ccctacgcca gcctgcactg gacgggggtt ttaacggtga ttattggatt
     8821 tatcctcgct tcggcattct ctgccattct ggtttacgct caggagctgc ttccgggacg
     8881 tatcggtatg gtttctggac tctttttcgg ttttgctttt ggcatgggag gtctgggagc
     8941 ggcagttctg gggcttatcg ccgatcacac cagcatcgag ttagtctata aaatctgtgc
     9001 tttcctgcca ctattgggga tgttgaccat attcctgcct gataaccggc ataaagactg
     9061 atttcattgc agccaaaggc aaactttggc tgcatcgttt tacagtcgcc ataagccttt
     9121 cctctgttaa accgccttct gttttccgat tcgtgctttt tttgcagcta attccatttt
     9181 tctgcaaaat tcaataatta ttcatgaaca tcatcaatga aattgtcata aactattacc
     9241 tgtaattttg gttttcccgg ccaaaaatgg acaataacca ccaggaaaag gagacggaat
     9301 gcatcacgcc accccgctta tcaccaccat tgttggcggc cttgtgctcg cctttatcct
     9361 cggcatgctg gccaataaac tacgtatttc tcctctggtg ggatatctgt tagcgggtgt
     9421 gctggcagga ccattcactc cgggctttgt tgccgatacc aagcttgcgc cggaactggc
     9481 tgaactgggc gtcattctgt tgatgtttgg cgtgggtctg cacttttcgc tgaaggattt
     9541 gatggcggta aaggccatcg ccattcccgg tgcgatcgcc cagatagccg tggcgacgct
     9601 gctgggtatg gcgctctctg ccgtgctggg ctggtcgtta atgaccggta tcgtgttcgg
     9661 tttatgtctt tccaccgcca gtaccgtggt gttactgcgc gcacttgaag aacggcaatt
     9721 aattgacagt cagcgtgggc aaatcgccat cggttggttg attgtggaag acctggtaat
     9781 ggttctgacg ctggtgttgc tgcccgcagt ggcaggaatg atggaacagg gcgatgtggg
     9841 ctttgccact cttgcagtcg atatggggat caccatcggc aaggtgatcg catttatcgc
     9901 cattatgatg ctggtaggtc gccgtctggt gccgtggatt atggcacgca gcgcggcaac
     9961 cggttctcgc gagctgttta ccctgtcggt gctggcgctg gcgttaggga ttgcctttgg
    10021 tgcggtggag ctgtttgatg tctcctttgc actcggtgcg ttctttgccg ggatggtact
    10081 gaacgagtct gaactgagtc accgtgccgc ccacgatacg ctgccattgc gcgacgcgtt
    10141 tgcggtgctg ttttttgtct ccgtcgggat gttgtttgat ccgttaattc tgattcagca
    10201 accgctggca gtgctggcga cgctggcgat tattctgttt ggtaagtcgt tagccgcatt
    10261 tttcctggtg cgactgtttg gtcactccca acgtacggca ttaactatcg ccgccagcct
    10321 ggcgcaaatt ggtgagttcg cgtttatcct ggcgggactg ggcatggcgc tggatctgtt
    10381 gccgcaagcc gggcagaacc tggtactggc aggtgcgatt ttgtcgatta tgctcaaccc
    10441 ggtcctgttt gcgctgctgg agaaatatct ggcgaagacc gaaacgctgg aagagcagac
    10501 gctggaagag gcaatcgaag aagagaagca gatcccagtg gatatttgca accatgcgct
    10561 actggttggt tacggtcgtg taggcagcct gctgggggag aaattgctcg cctctgatat
    10621 tccgctggtg gtgattgaga cgtcacgaac ccgtgttgat gagctgcgag agcgtggggt
    10681 ccgcgctgta ttgggcaatg cggcgaacga agaaattatg caactggcgc atctggaatg
    10741 tgcaaaatgg ctgatcctga cgattcccaa cggttatgaa gcaggcgaga ttgtggcatc
    10801 tgcccgcgcg aaaaatccgg atattgagat tattgcccgc gcccattatg acgatgaagt
    10861 ggcgtatatc accgaacgtg gcgcgaatca ggtagtgatg ggtgaacgtg aaattgcccg
    10921 tactatgctg gaactgctgg aaacgccacc ggcgggtgaa gtggtgacgg ggtaaacggt
    10981 ggttgccgga taataggcgc ggatatgtag gcctgataag acgcgccagc gtcgcatcag
    11041 gcattgagag caaaatgcca gatgcggcgt gaacgcctta tctggcctac gaaccgacga
    11101 taacttaacg atcccagtaa gactcttcca ggctgtcttc acgctccggc aagccgcgcg
    11161 ttaaacgagg tgaatgctgg ttcagtacct gatagctcac acggttagca tatttacaca
    11221 cctgcgctaa cgatgaataa gttaaccagg tgaatttatg tttgctggag tttggtacgt
    11281 tgctacggtg gtagctgttg gcggtaatgt catgcagcaa cgctgccagt gcgccatccc
    11341 ctgctccgtt ggtgttcatg attttttccg gcccgcccat gtacggcgca atgtgcgaat
    11401 agacgcgcag cggattctgg caatccttgt ggcgcatggc gcggctaaac tcatactggt
    11461 tgaattccgc tatagcgccc ggcagcagcg gatgctgggt tttacgtttc gcttcgtctt
    11521 cggtaaagcc cgccatatac aagccgattg gcccggcggt gcacagcacc agatctaccc
    11581 agtccagcgc cttgtcagat gccaacaacg gatcgctttc tccggtcaac gcttcggctt
    11641 catcttcgtt catcgcaagg atagagacgt ggtctttgag gaattgctgc caccactgtg
    11701 gattctcggc aatgacaaac ttagttccca gcgtcagcac caccggtacg ttatatttct
    11761 tcgcgtactc aatggctttc atggttgctt ccggcatggg ttcacccggc ttgcaacgca
    11821 ccagataaga ggtgagaacc agagccgagg ctccggcaat cacatcttcc ggaatacttt
    11881 cagcccgcag ctggttcatg tggccagggc tgatagcaaa ggtacgttcc ccggactcgc
    11941 caatcagcgt aaagcaacga ccaatcgggc catccacgcc ttgtagatag ttaagatcgg
    12001 tacggctgga ggtgttacac aggtaacgat aggcatagct gccaatttca atattgctgc
    12061 acatgacgcc cagcagcacc gaacggtcgt ccgcgagcac cgagtagtta tgcatggtgt
    12121 taccaatggt gccacccgca aactgatggg taatcaggtt tttctgtttt agttcctgat
    12181 aaagcgcttc ggctacatca tcctcaatca ccagtgaatg cccggcgctt aatccataac
    12241 gctcaatgaa ttcatcatcc actttcgctt caatatcgac cagcgtttga tcgataccca
    12301 ctacccaggc ggcgctggtt tcgttttctg gctggaattg ctgaagcagc ggatcgcgtg
    12361 catttacggg gaagtaatgt ttggatttac gtttaccggg aaatttcatg gttgttttta
    12421 cgggtggtta actgagcggc gaatgttaac acaatctgac gggagcgcgc gagacgcagc
    12481 cagtttcaat atcagcaggc gggatcgcat tttcatggca gtggttcctt acaatgacgt
    12541 aatttgaaag gagtttttgt atgaagccgg aaaacaaatt acctgttctg gaccttattt
    12601 ctgctgaaat gaagatcgtt gtgaatactc ttcagccgga tttaccgccc tggcccgcaa
    12661 cgggaacgat tgctgagcaa cgacagtatt acacgcttga gcgccgattc tggaatgcgg
    12721 gcgctccaga aatggcaacc agagcttaca tggttccaac aaaatatggg caggtggaaa
    12781 cacgtctctt ttgtccgcag ccagatagcc cggcaacgct attttatttg catggaggcg
    12841 gttttattct cggcaatctc gatacccatg atcgcatcat gcgcctgctg gcaagctaca
    12901 gccaatgtac ggtgattggc attgattaca ccctttcacc tgaagcgcgt tttccgcaag
    12961 cgatagagga aattgtggct gcctgttgtt atttccacca gcaggcggag gattatcaaa
    13021 tcaatatgtc ccgcattggc tttgccggtg attccgcagg tgccatgctg gcgctcgcca
    13081 gtgcgttgtg gttgcgtgat aaacagatcg attgcggtaa aattgcgggt gttttgctgt
    13141 ggtatgggct ttacggatta cgggattccg tgactcgtcg tctgttgggc ggtgtctggg
    13201 atggcttaac gcaacaggat ttgcagatgt atgaagaggc gtatttaagc aactacgcgg
    13261 accgcgagtc gccgtattac tgtctgttta ataatgatct cactcgcgaa gttccgccct
    13321 gttttattgc cggggcggag ttcgatccgc tgctggatga cagccgtctg ctttaccaga
    13381 cgttagcggc gcatcagcag ccctgtgagt tcaaactcta cccaggcacg ctgcacgcct
    13441 ttttgcatta ttcacggatg atgaaaaccg ccgacgaggc tcttcgcgac ggcgctcagt
    13501 tctttaccgc tcagctttag cgatacgcgg caacaagatt agccatcatt tcgatatgtt
    13561 ccggcgtggc attaagcgcc ggaatatatt catatttttt cccgccggca ccgaggaaga
    13621 cctcacggtt ttgctcggca atctcttcca gcgtctccag acaatccgca gcaaagcccg
    13681 ggcacatcac ctgtatatga cctacgcctt tttctccgag cattttcagc gtttcgtcgg
    13741 tataaggcat cagccagggt tcccgaccaa agcgcgactg aaaggtcatc atcacttttt
    13801 ccggtgccat ccccagtgcg gaagccagtt cgcgagtcgt tgtgcggcaa cgttgcgggt
    13861 aatcatcgcc ttcatctgca taacgctggg gaatgccatg ataagagagc agcagcagat
    13921 ccggttcgcc atgtttggca aaagaagcgc gtacgctgtt cgccagtgca ttaatgtaat
    13981 cgtggttatc ggcgtaatca cgtataaacg agatccccgg aatgctacgt ttgcgcgcca
    14041 gaatgcgtgc cagttcatcc cataccgcac cgaccgtaga acaggagaat tgcggataaa
    14101 gcggcagcac cacaatatga tctacatgct ctgccaggag ttcatctacg gcgctttcca
    14161 gtgatggcga gccgtagctc attcccagcg ctacgggcgt ctccggtaaa cgttgtgcca
    14221 gcgcctgttg ttgctggcgg ctgtaaacca tcagcggcga gccaccttcc atccagacag
    14281 aggcatacag cttcgccaca cgcggcgagc gcagcggcaa aatcacgccg cgcagcaatg
    14341 gccaccataa caaccgtgag gtatcaacca cgcgtctgtc gcttaaaaat tgtttcagat
    14401 agcgttttac cgcttcaggt gtgggggcat cgggcgtacc caggtttgcc agcaggatac
    14461 cggttttagt ctgacgcatt accgcctctt atcgattcaa cttgttgata attgtagcgg
    14521 aaaagtgagg aagaagaact aattgctgtg agaggtattg cctgaggtgg atgagcagac
    14581 ctgcccgaaa gcagatctgc tgcggcgtga ttagccgagg attttttcca gatcagcgcg
    14641 aacttcagca accggcttgg tgccgtcaac tttcgcgtat ttggtattac ccgcttccgc
    14701 ttctttggag tagtagccga tcagcggtgc tgtcatctga tggtattcaa ccagacgttt
    14761 acgtacggtt tcttcctgat cgtctttacg ggtagtcagt tcttcaccgg taacgtcgtc
    14821 tttaccttct actttcggcg gattgaattt aacgtgataa acacgaccag acggcgcgtg
    14881 aacgcggcga ccgacgatac ggtcaacgat cagttcgtcc ggtacgtcga attccagaac
    14941 gtaatcaaca ttgatgcccg cttctttcat cgcgtctgcc tgcggaatgg tacgcgggaa
    15001 gccgtccaac aggaaaccat tacggcagtc ttcctgagca atgcgctctt taaccagcgc
    15061 gatcaccagt tcgtcggtga ccagtttgcc agcatccata atgtcttttg cttgtttacc
    15121 cagctcggag ccagatttga ccgcagcacg cagcatatcg ccagtggaga tttgcggaat
    15181 accatatttc tccatgatga actgagcctg agtccctttc cccgcgcccg gagcgccaag
    15241 cagaatgata cgcattgcga aaatcccctt aaaatgtgtc gaattttttg aaaaagcgat
    15301 aaacgatacc accatcaggc gattgcctca agaaaggcac caggggctga aacggttaat
    15361 ggcgagataa tttgcggaaa gtgcagataa tgaaaagaaa aatgccggat gacacgaagg
    15421 tcatccggca ttacatcagg aaaccagcag ctggttcata cgacgaataa acaggttcgg
    15481 atcttccagc gtgccgcgtt ctgccagcag tgcctgatcc agcagcagtt ctacccactc
    15541 gctgaacttc gcttcatctt cagtatctgc cgcacgtttc accagtacgt gatccgggtt
    15601 cagttcgaag atgtatttca cttctggcac tttctggccc gccgcagcga acagtttcgc
    15661 catctgagtg ctcatttcgt ccgcatcagt agagacaatc gctggcgtat cggtcagacg
    15721 gtgagtcaga cggacatctt tcacgcgctc gccgagcagg gctttcacac ggtcgatgaa
    15781 cggagtcagt gctttctccg cttctttcgc gctctcatca acttcgtcag ccagtttttc
    15841 cagcgactcg tcaactttag acaccgactg gaacggttta ccgtcgaact cagtcagata
    15901 gttcatcatc cactcatcga tgcggtcgga aagcagcaga acttcgatgc ctttcttacg
    15961 cagcagttcc aggtgcgggc tgctcttcgc tgccgcatag ctgtctgcag tgatgtagta
    16021 gattttctcc tgcccttctt tcatgcggga aacgtagtct tccagagaca cggtctgcgc
    16081 agaagaatcg gtatgagtag aagcaaaacg cagcagtttg gcgatcgctt cctggttagc
    16141 gaaatcttcc gccggacctt ctttcagtac caggccaaac tgttgccaga aggtctggta
    16201 tttttccgcg tcgtctttcg ccagtttttc cagcatttgc agcacacgct tggtcagcgc
    16261 attgcgcagg ttacgcgtta ccgtgctgtc ctggaggatt tcacgggaaa cgttcagcgg
    16321 cagatcgctg gagtcaatca gaccacgcac gaagcgcaga tagttcggca tgaactgttc
    16381 tgcgtcgtcc atgatgaaca cacgctgaac atacagtttc aggccgtgtt tatgatcacg
    16441 gttccacata tcccacggag cctgggacgg gatgtacagc aggctggtgt actcctgctt
    16501 accttcaacg cggttgtggc tccaggtcag cggatcgtta aagtcgtggg cgatgtgttt
    16561 gtagaactct ttgtactctt catcggtgat ttccgacttg ttacgagtcc acagcgcctg
    16621 cgctttgtta attttctccc aggagataac ggtttcgccg tctttctctt cgcgtttttc
    16681 gatctctacc ggcagcgcga tatggtcgga gtatttgctg atgatggaac gcacgcgcca
    16741 gtcatcgagg aactcgtctt cgccttcacg cagatgcagg gtgatttcag taccacgatc
    16801 ttctttggtg atgtcggcaa cggtatattc accttcgcca gccgattccc agaagacgcc
    16861 gttttctggt ttttcgcctg ccgcgcgagt acgcacggtc actttgtcgg ccacgataaa
    16921 cgcagagtag aaaccaacac caaactgacc gatcagctga ctgtctttcg cctggtcaga
    16981 acccagcgat tcaaggaatg atttagtgcc ggatttagcg atagtcccca gatggtcaat
    17041 cacttcgtcg cgggtcatcc ccacgccgtt atcggagatg gtcagcgtac gcttgtcttt
    17101 atcgaaagag acacgaacgc gcagttcgcc atcaccttcg tacaggtccg ggttagagag
    17161 cgcgcggaaa cgcagcttgt ccgccgcatc ggaggcgtta gagataagct cacgcaggaa
    17221 gatttcttta ttggaataga gagaatggat catcaggtgc agaagctgtt tcacttctga
    17281 ctgaaaacca cgagtttctt gtcctttcat gtaggtctac ctcaataatg ccattttaag
    17341 gttaaaaaca ggatgaggga gagatgggga caagggagaa taatttcaag cgagagcagg
    17401 tgatcctgct ctcgtttgct taaaaacgaa tcttatgacg cccggcaagg gagtgtgaca
    17461 gcgtggtgcc atcgaccatt tccagctcgc cgccaaccgg tacgccatga gcgattcggc
    17521 tggcttccac gtcatattgc gcgcaaagct cggcaatgta gttagcggta gcttcacctt
    17581 caaccgtagg gttggtggcg aggatcactt cagtgatttt ttcctctgcc agacgctgtt
    17641 ccagacgatc aaggccgata tcatccggac cgatgccgtc cagcggtgac agatgtccca
    17701 tcaacacaaa ataacgacct gaaaactgcc ccgtctgctc aatggcgtaa atgtccgccg
    17761 gactctccac cacgcagatt tgaccgtttt cctgacgacg cggattcgaa cagatgttac
    17821 agacttcctg ttcggtgaaa gtgcggcaat cggcgcagtg gccgatttcc gacatcgccc
    17881 gggtgagcgc ctgcgccaga cgcatcccgc cgctacgatc gcgctgaagc agcgtgaacg
    17941 ccatacgctg cgccgacttc gggccaacgc ccggcagaca gcgcagtgct tccataagct
    18001 gtgttaacag cgggctggtt tgcatcagaa cggcatctta aagccaggcg gcagctgcat
    18061 tccggaggat acagaggcca ttttttcttt ctgcgtttct tcaatacgac gtgctgcgtc
    18121 gttgaatgct gcagccacca ggtcttccag catctctttg tcgtcttcca gcaggctcgg
    18181 gtcgatctct acgcgacggc agttgtgtgc accgttgatg gtcactttta ccagacctgc
    18241 gccagattcg ccggtgactt ccagctgcgc gatctcttcc tgcattttct gcattttttc
    18301 ttgcatctgc tgggcttgct tcatcaggtt acccagaccg cctttaccaa acataggttt
    18361 ctctctcaat cacgttaagg atgacgaacg taagctgtgc ttacgatcaa atggggcgga
    18421 tactttcttc atccagctcc gcatcgaaga atcgacgcag ggtctgaata ttattatccg
    18481 caataatgga ctcgcgcgcc tgcgcaagtt tttcttcgta tatcgcctga cgccactcca
    18541 gcggcgtacg caccgcggga ttatcatctt caacgatagt cagttcaacc gttgaacctt
    18601 ttaacatgct caacgcttca gccagttttt gctgtgcacc gcggttgttc aaatgccgct
    18661 gagaggagcg caaatgcaga catactgcgt tatcactctc ctctttccag gcatttaagg
    18721 ccacctgttc gaccagtttt ggtagcgaaa gttgactcac ctgagccgcc cacgcgtcgc
    18781 gctcaatggc ttctgccgcc agcttcgccg ccagttccgg cgttttttca tgttccagcg
    18841 cttttttcag cgccttcggt gtggcgacca cttctttttg ctgcatcact ggagtggtcg
    18901 ccttccagcg atacgcttct tttttagctg gcgctttttc cagcgccgat ggcaccggac
    18961 gcgcctgaac gcgatccgtg accgaagcca gtctttccag cgcagcgtta ttcaccggcc
    19021 gcgcgcgggt agcggctgcc ggttcactct tttttgcttt ggttgctccc tgcacgcgct
    19081 gcaactgctg gcgcgccgcc agcacctggc tggtggtttc cgggagcggt acagtcggtg
    19141 cctgctgcgg cgctgattgc ggttgcggcg gcacctgggt tggcgtcatt actgccgttg
    19201 gcgcgacggg tgcaaaggac tggcgtggca cttctggctc aggcagcggc atacgcggat
    19261 ggaacgccag cgcgcgcagc agcgtcatct caacgcccat gcggcggtcc ggcgcatacg
    19321 gtaattcttt gcgaccaatc aacagcgtct gatagtaaag ctgaatatcc gtcggcggta
    19381 tggtgcgcgc cagttcacgc atccgcagct cgatggcggc catgtcgttg ccaagtgcag
    19441 caggcgaaag ttgtaccatc gcaatacgat gcaacaggcc gagcatttcc accagcaacg
    19501 cttcccactc gataccacgg gcagcggctt cattaatcag cgccattacg cgctcgccgt
    19561 tggcctcgac catcgcttca accagcgaca gcgcctgatc gtcgtcaagc gtacccagca
    19621 tcgcactgac cgcctgggtt gaaacctggc cgtcaccgct ggcaatcgcc tggtcggtca
    19681 gacttaaggc atctcgcagg ctgccttcag cggcgcgcgc cagcaattgc agcgcccgcg
    19741 gctcgtgagc gatatgttct tcattgagga tgtgctcaag ttgatggcga atttgctcga
    19801 catccagcgc cttgagatga aattgcaaac agcgcgacaa aatcgtcacc ggcaatttct
    19861 gtgggtcggt cgtcgccagc agaaacttaa cgtgctccgg cggctcttca agagttttta
    19921 acagtgcgtt aaagctgtgg cgcgacagca tatgcacttc gtcgatgaga taaactttga
    19981 aacgaccacg cgccggagcg tactggacgt tatccagcag gtcgcgagtg tcttcaactt
    20041 tggtgcgcga ggcggcgtcg atttcaatca gatcgacaaa gcgcccctgc tcgatttcac
    20101 gacagttatc gcacacgccg cacggcgtcg cggtaatgcc ggtttcgcag tttagcccct
    20161 tcgccagcag tcgggcgata gaggtttttc cgacgccacg ggtaccggaa aaaagataag
    20221 catgatgaat acgccctaac gacaagccgt tcgccagtgc ggtcagcaca tgttcctggc
    20281 cgacgacgtc agcaaaggtt tgtgggcgcc attttcgggc taagacctga taactcattg
    20341 gcaggctctg aaacgctgga aggtggattc acgaaggggt aatgctaaca cagccccgtc
    20401 agaacggcga ggctgaatgc acatcttgtg ctgtccgtag cgtgggcagc acaagactgg
    20461 cgataattaa tggcccggga acgggacaag gctgtagctg gtaatgccct gtttttcgag
    20521 acgctgttcg ccgccgagat cgaacaggtt gataatgaac gcagcgtcag ccacttcacc
    20581 acccagacga cggatcagtt taacggtcgc ttcgatagtg ccgccggttg ccagcagatc
    20641 gtccaccacc agaactttgt cgcccggttt gatggcatca acgtggatct ccagctgatc
    20701 ggtgccgtat tccagatcgt aagtttcact gatagtttca cgcggcagtt tgcccggttt
    20761 acggaccggt acaaagccaa cgcccagacc cagagctacc ggagcgccaa acaagaagcc
    20821 acgcgcttcg gtgccgacaa ctttggtaat gcccgcattt ttgtaacgct caaccagcaa
    20881 gtcgatgctg agagcgtaag ctttcgggtc ttccagtaag ctggtgacat cgcggaaaag
    20941 aatgccgggt tttgggtagt cctgaatgct tttgatgcta tttttgagat actcaagctg
    21001 ctgtgcagtc gcggtcataa gtgtatgcct gcttgttacg gtggtactca cggcgcgttt
    21061 taaacgtatc aaaagtaaca gttgtttaac ctttgaccgc ctgcgcctgt gctcgaaaac
    21121 gccagaatgt actggctgta cgcaataatt gcaactgcga ttgttgtgct tcagtgcttt
    21181 tcttgctttt catcaatcac cggaattcgc cacatataga aaagcaaaca ggcgagaatt
    21241 accagcaaca tgatgcgcac ccatggcatc tggacgaacc acagagaaat ggcaaacgtg
    21301 agcaaaataa gcaaaatcgc ccgcggtttg acgccgcgcg gcatcgcatg atgtttctgc
    21361 cagaaacgta gatagctgcc aaaccatgag cggtacagca accaggcgtg aaagcgcggg
    21421 gaagaacggg caaagcacca ggccgctagc aggataaacg gcgtcgtcgg taataccggt
    21481 aataccacgc ccagcgtacc cagcactacc gccagccagc caatgatgat taaaatgatt
    21541 cgttgcatat tgagtgttgt cagcttacag agtggctact ttagcataac aattatcatt
    21601 ttcattgagg tcttatcgtg aaaaccgccc tgctgctgga aaaactggaa ggacagctcg
    21661 ccacgctgcg ccagcgttgt gccccggtag cgcaatttgc cacgctaagt gcccgctttg
    21721 acaggcacct ttttcagacc cgtgcgacaa cactgcaggc ttgtctcgat gaggcgggcg
    21781 ataatctggc tgcgcttcgt catgcagttg agcaacagca actgccgcaa gtcgcctggc
    21841 tggcagaaca tctggcggcg caactggagg ctatagcgcg tgaagccaca gcctggtcat
    21901 tgcgcgagtg ggacagtgcg ccaccgaaaa ttgcccgctg gcagcgtaaa cgtattcagc
    21961 atcaggattt tgagcggcgg ctacgtgaga tggttgccga acgtagagcc cgcctggcgc
    22021 gggtgaccga tctcgtggaa cagcaaacgc tgcatcgtga agtggaagcc tatgaagcgc
    22081 gcctggcgcg gtgtcgccat gcgctggaaa aaatcgaaaa caggttagcg cgtttaaccc
    22141 gctagcaatg gagagagtat gtcactggaa aatgcccccg acgatgtcaa actggccgtc
    22201 gatttgattg tgctgctgga agaaaatcag atcccagccc gcaccgtgct gcgcgcgctg
    22261 gatattgtaa aacgcgatta tgaaaagaaa ttaacgcgcg atgatgaggc ggaaaagtga
    22321 gaaataaatg gggtggctaa atgtccccta aggttgcggg caaacgcaaa actgcctgat
    22381 gcgctacgct tatcaggcct acaccgatcc tgcaatatat tgaatttttg cggtttcgta
    22441 ggccggataa ggcggtcacg ccgcatccgg catgaacaac gcgcactttg ttagcaatct
    22501 cggggcaaca aaatgtcgcc ccctttcgtt attaccctac cgctggcgtc gggtcatcgc
    22561 ctttgtagtc gcgttttact tccgtcacct catcgccctt ctcgttgtgc agatgcactt
    22621 caagctggtt aaaggcaatg ttgatgtcgt tttcacggca cagctgatcg atagtacggt
    22681 tcagctcatc gacagtacgg ctacggtcac gcagttcacg cacatacaga cgcagctcat
    22741 gatccaacgt gctggcacca aacgccgtaa agaagacttc cggcattggt tcgtgcatca
    22801 cccttgggtg ctcagtcgcc gccttcagta acactttacg cactttttcc agatcggagc
    22861 cataggccac gccgagacgg atcaccagac gcgtagtagt gtcagtcaac gaccagttga
    22921 tcagacgctc ggtaacaaac gctttgttcg ggatgatcac ttctttgcga tcgaaatcgg
    22981 taatcgttgt cgcacgaata cggatcttac ttaccgtccc cgagaagcta ccaatggtta
    23041 ccgtatcgcc aatacgcacc ggacgttcga atagaatgat caaaccggag acgaagttac
    23101 cgaaaatttc ttgtaaacca aaaccgagac ctacggataa tgctgctgcc agccactgga
    23161 gtttatccca agagacgccc agcgatccga acaccgtcat cgcaccaaca gcaataatga
    23221 tgtagttaag gatggtagta atggcatacg acgcgccctg gcgcatattc agtcgcgaga
    23281 gcaccagcac ttccagcaaa ccaggcaggt tgcgaatcaa cgcccaggcc accattgagg
    23341 cgataatcgc aaacaacaga ctgcccatgg tgacgttttt caccaccgca gcaccagctt
    23401 cagtaccgtt gtaatgccac agcgtaatgc tgtcgagata gctgaacacg gtgatcaaat
    23461 cggaccaaat tgcccagaac atgacaccga acagcgcaaa catcagcaac atggtaatac
    23521 gcagcgtctg ctggttaacc tgttccagtg caatggtggg ttcttccggc ggttcggcac
    23581 cttctgcgcc ctctttcacc agattctgcc gacgcgccag cgcacgacgc caggcgatac
    23641 gccgcgccgc tacgcttaag ccgcgcaata ccgtctggta gagcaagttc cagatgatca
    23701 ccagataaac ggtttcaatc caacgcccgg agaggcgcag cgtggtgtag aaataacccg
    23761 tggcagtcag caccatcaat gcaatcggga taatcgacag cacggtaatg gtgaccagtc
    23821 gcatggtgtg cgactcttta tcacgccagc tttcgcggca cattggccat accaggaagg
    23881 caatcagcag caggttgaag aaaatcatcg cctgccccag cacatcatcc atcagatgca
    23941 gcggggaaag ttctgccacc acagaccaga aatggatagg cagcaacgcg agactgatgc
    24001 ggacaatttg ccgacgccag tggctggtct gctgctccgg catgccgaag tgacgcacgg
    24061 caacgccatt tttctccagt accttccagc acaggccaaa caccagccag aaaatcgcca
    24121 gttttttact gaacgaccat agcagttcgc tgatgttgag ctgcatggtc aacagaatca
    24181 ggccaaccgc gagaataatc aggcacaccg gcagcgcacg gatcaggtcg ataaggatcg
    24241 cttttggtgt gttgagctgg ctgtcgttac gcagggaacc cacagcggaa gccagttttt
    24301 gttgatacgc tttcagccac cccagacgcc agtggatcag cccggcaatc aacagcagcg
    24361 gcaaaccagc gaggaaagcg ataaaaacgg cgggccaggc tttttcccag ttcaccttaa
    24421 ttttcatcga cttaaattca tctttcaggc tttgcgggaa cgctttgatc cagtcccagt
    24481 ccattggacg gttactgttc acccaaaaga tttgctgagt caggatggat ttcaggtttt
    24541 tcgacacact cattaactgc tgctggttga tttgcaggtt aatggccatc atcagctggt
    24601 tacccaactg tttgttgagt tgatccagca attcgcgacg catatcaacc acttgcaata
    24661 acgcatcgtg aacttcgctg ttgacttcgt tggtgtgacc ttcttccagt ttgttgacga
    24721 acgcatcgct ctggaagagt gcatcacgct gctggttaac ttcaaactgt tcgagacgca
    24781 aatccgcgat gcggttggtc atgttttcca gttcatccgc cgagggcagc gtttgttgtt
    24841 gctggtaaag gatacgagac aacagcaggc tgcccttcag gacggcaatc tgctctttaa
    24901 tattgcgttc cgattgcagc gcccgctcca gccagttttt gactttaatg ttttgctgca
    24961 tcaactgatt accgttttca gtcgcggtaa tcagacgctg acttaactgc tggttaattt
    25021 ccagttcctg cttcaccagc ggattagcct gaatacgcgc ggcttcatcc ggggagacgg
    25081 cttcctgcgc cgttttttcg gttaaagtca ggcgcttgct gtttaccgct tcttgcaaca
    25141 gttgtaactg gtgctccaga cgggcgctgt tcgccgtcac gtaatcacgt tgcttttgca
    25201 aggtatcctg caagacggta ttcccttcca ggcttttacg ctgctggtca atctccgcat
    25261 tcagcaacgc ctgctgagcc tgcattaaca ctttctggct gggacgtaag gcagtctcgc
    25321 cgacatcagt cccatccaga cgactgcgaa tttgttgcag ctgctgcgaa gcgttataca
    25381 tcgcattttg cacgcgttcg ggctgcgtct gtaacgaaac cagctggctg ttataagacg
    25441 ctagatcgtt ttgtgcgttt tgcaaatcgt ccagcgcctg ggcaacgcga gtttccagct
    25501 ggcgcaacga aagcgtgctc agaattttgc gcgtttcttc gtcgttatcg acatcgctaa
    25561 gtgctgttaa cgccgcggtc gcctggcgca ttttttccgg cgcttcagcg actttttgcc
    25621 gtagctgaac ggtctcttct tttacgcgat cgattttatc gagggtggct aatgtatctg
    25681 tcagatcctg ctgcaccagt ttgtcctgag cagaaagatc tttttgttta tttagtgagt
    25741 caagttgcgc ctgcaggtcc gcttttgtcg gcagatcacc attcgatgac gcccgcgcaa
    25801 acgccgtgtt ctggcataac aagacaaaaa caaaagcaat aaatgctgaa aaaacaaaat
    25861 gccgtgatcg tttgtaatac tggaacatag tcatgatgaa tgaaggtttc tgaacctgaa
    25921 gaacgacctg aaaaagtcaa accgcaagaa tatcacgacg cagtgaacca gaatagcaac
    25981 gacgaaaatg tccaggaaaa atcctggagt cagattcagg gttattcgtt agtggcaggg
    26041 ttacgaagcg tggggcacag gagatacatc tccagtaaga tggcgacgta atcgcgggct
    26101 tcttttttaa gatcaaaaga ttgcggggca aagagccagt tttccatcag gccggaaata
    26161 tagccgcgca taataattgc tgcgcgacgc gtcattaaat ccgcaggcaa cattttcgct
    26221 tcaatacaat gttttaacgt ttgttctata cggtcataac tttccagaca gagattacgt
    26281 tgtgcctgtt gcacaacagc catttctccg acaaattcgc atttgtggaa tataatctcc
    26341 atcaataatc gacgccgttc ttctgtcacc gtggattcaa gaacatgaat taatatctct
    26401 cttaatactg agagtggatc gccagggaat tttgcctgat actcaagctc tagttcacca
    26461 atattggatt ctgacagttc ccagatctca ctgaacaaat ccgacttgtc tttaaaatgc
    26521 cagtagattg caccgcgcgt aacgccagct gcttttgcaa tctcgcccag cgaggtggat
    26581 gataccccct gctgtgagaa aagacgtaga gccacatcga ggatgtgttg gcgcgtttct
    26641 tgcgcttctt gtttggtttt tcgtgccata tgttcgtgaa tttacaggcg ttagatttac
    26701 atacatttgt gaatgtatgt accatagcac gacgataata taaacgcagc aatgggttta
    26761 ttaacttttg accattgacc aatttgaaat cggacactcg aggtttacat atgaacaaaa
    26821 acagagggtt tacgcctctg gcggtcgttc tgatgctctc aggcagctta gccctaacag
    26881 gatgtgacga caaacaggcc caacaaggtg gccagcagat gcccgccgtt ggcgtagtaa
    26941 cagtcaaaac tgaacctctg cagatcacaa ccgagcttcc gggtcgcacc agtgcctacc
    27001 ggatcgcaga agttcgtcct caagttagcg ggattatcct gaagcgtaat ttcaaagaag
    27061 gtagcgacat cgaagcaggt gtctctctct atcagattga tcctgcgacc tatcaggcga
    27121 catacgacag tgcgaaaggt gatctggcga aagcccaggc tgcagccaat atcgcgcaat
    27181 tgacggtgaa tcgttatcag aaactgctcg gtactcagta catcagtaag caagagtacg
    27241 atcaggctct ggctgatgcg caacaggcga atgctgcggt aactgcagcg aaagctgccg
    27301 ttgaaactgc gcggatcaat ctggcttaca ccaaagtcac ctctccgatt agcggtcgca
    27361 ttggtaagtc gaacgtgacg gaaggcgcat tggtacagaa cggtcaggcg actgcgctgg
    27421 caaccgtgca gcaacttgat ccgatctacg ttgatgtgac ccagtccagc aacgacttcc
    27481 tgcgcctgaa acaggaactg gcgaatggca cgctgaaaca agagaacggc aaagccaaag
    27541 tgtcgctgat caccagtgac ggcattaagt tcccgcagga cggtacgctg gaattctctg
    27601 acgttaccgt tgatcagacc actgggtcta tcaccctacg cgctatcttc ccgaacccgg
    27661 atcacactct gctgccgggt atgttcgtgc gcgcacgtct ggaagaaggg cttaatccaa
    27721 acgctatttt agtcccgcaa cagggcgtaa cccgtacgcc gcgtggcgat gccaccgtac
    27781 tggtagttgg cgcggatgac aaagtggaaa cccgtccgat cgttgcaagc caggctattg
    27841 gcgataagtg gctggtgaca gaaggtctga aagcaggcga tcgcgtagta ataagtgggc
    27901 tgcagaaagt gcgtcctggt gtccaggtaa aagcacaaga agttaccgct gataataacc
    27961 agcaagccgc aagcggtgct cagcctgaac agtccaagtc ttaacttaaa caggagccgt
    28021 taagacatgc ctaatttctt tatcgatcgc ccgatttttg cgtgggtgat cgccattatc
    28081 atcatgttgg caggggggct ggcgatcctc aaactgccgg tggcgcaata tcctacgatt
    28141 gcaccgccgg cagtaacgat ctccgcctcc taccccggcg ctgatgcgaa aacagtgcag
    28201 gacacggtga cacaggttat cgaacagaat atgaacggta tcgataacct gatgtacatg
    28261 tcctctaaca gtgactccac gggtaccgtg cagatcaccc tgacctttga gtctggtact
    28321 gatgcggata tcgcgcaggt tcaggtgcag aacaaactgc agctggcgat gccgttgctg
    28381 ccgcaagaag ttcagcagca aggggtgagc gttgagaaat catccagcag cttcctgatg
    28441 gttgtcggcg ttatcaacac cgatggcacc atgacgcagg aggatatctc cgactatgtg
    28501 gcggcgaata tgaaagatgc catcagccgt acgtcgggcg tgggtgacgt tcagttgttc
    28561 ggttcacagt acgcgatgcg tatctggatg aacccgaatg agctgaacaa attccagcta
    28621 acgccggttg atgtcattac cgccatcaaa gcgcagaacg cccaggttgc ggcgggtcag
    28681 ctcggtggta cgccgccggt gaaaggccaa cagcttaacg cctctattat tgctcagacg
    28741 cgtctgacct ctactgaaga gttcggcaaa atcctgctga aagtgaatca ggatggttcc
    28801 cgcgtgctgc tgcgtgatgt ggcgaagatt gagctgggtg gtgagaacta cgacatcatc
    28861 gcagagttta acggccaacc ggcttccggt ctggggatca agctggcgac cggtgcaaac
    28921 gcgctggata ccgctgcggc aatccgtgct gaactggcga agatggaacc gttcttcccg
    28981 tcgggtctga aaattgttta cccgtacgac accacgccgt tcgtgaaaat ctctattcac
    29041 gaagtggtta aaacgctggt cgaagcgatc atcctcgtgt tcctggttat gtatctgttc
    29101 ctgcagaact tccgcgcgac gttgattccg accattgccg taccggtggt attgctcggg
    29161 acctttgccg tccttgccgc ctttggcttc tcgataaaca cgctaacaat gttcgggatg
    29221 gtgctcgcca tcggcctgtt ggtggatgac gctatcgttg tggtagaaaa cgttgagcgt
    29281 gtgatggcgg aagaaggttt gccgccaaaa gaagccaccc gtaagtcgat ggggcagatt
    29341 cagggcgctc tggtcggtat cgcgatggta ctgtcggcgg tattcgtacc gatggccttc
    29401 tttggcggtt ctactggtgc tatctatcgt cagttctcta ttaccattgt ttcagcaatg
    29461 gcgctgtcgg tactggtagc gttgatcctg actccggctc tttgtgccac catgctgaaa
    29521 ccgattgcca aaggcgatca cggggaaggt aaaaaaggct tcttcggctg gtttaaccgc
    29581 atgttcgaga agagcacgca ccactacacc gacagcgtag gcggtattct gcgcagtaca
    29641 gggcgttacc tggtgctgta tctgatcatc gtggtcggca tggcctatct gttcgtgcgt
    29701 ctgccaagct ccttcttgcc agatgaggac cagggcgtat ttatgaccat ggttcagctg
    29761 ccagcaggtg caacgcagga acgtacacag aaagtgctca atgaggtaac gcattactat
    29821 ctgaccaaag aaaagaacaa cgttgagtcg gtgttcgccg ttaacggctt cggctttgcg
    29881 ggacgtggtc agaataccgg tattgcgttc gtttccttga aggactgggc cgatcgtccg
    29941 ggcgaagaaa acaaagttga agcgattacc atgcgtgcaa cacgcgcttt ctcgcaaatc
    30001 aaagatgcga tggttttcgc ctttaacctg cccgcgatcg tggaactggg taccgcaacc
    30061 ggctttgact ttgagctgat tgaccaggct ggccttggtc acgaaaaact gactcaggcg
    30121 cgtaaccagt tgcttgcaga agcagcgaag caccctgata tgttgaccag cgtacgtcca
    30181 aacggtctgg aagatacccc gcagtttaag attgatatcg accaggaaaa agcgcaggcg
    30241 ctgggtgttt ctatcaacga cattaacacc actctgggcg ctgcatgggg cggcagctat
    30301 gtgaacgact ttatcgaccg cggtcgtgtg aagaaagttt acgtcatgtc agaagcgaaa
    30361 taccgtatgc tgccggatga tatcggcgac tggtatgttc gtgctgctga tggtcagatg
    30421 gtgccattct cggcgttctc ctcttctcgt tgggagtacg gttcgccgcg tctggaacgt
    30481 tacaacggcc tgccatccat ggaaatctta ggccaggcgg caccgggtaa aagtaccggt
    30541 gaagcaatgg agctgatgga acaactggcg agcaaactgc ctaccggtgt tggctatgac
    30601 tggacgggga tgtcctatca ggaacgtctc tccggcaacc aggcaccttc actgtacgcg
    30661 atttcgttga ttgtcgtgtt cctgtgtctg gcggcgctgt acgagagctg gtcgattccg
    30721 ttctccgtta tgctggtcgt tccgctgggg gttatcggtg cgttgctggc tgccaccttc
    30781 cgtgggctga ccaatgacgt ttacttccag gtaggcctgc tcacaaccat tgggttgtcg
    30841 gcgaagaacg cgatacttat cgtcgaattc gccaaagact tgatggataa agaaggtaaa
    30901 ggtctgattg aagcgacgct tgatgcggtg cggatgcgtt tacgtccgat cctgatgacc
    30961 tcgctggcgt ttatcctcgg cgttatgccg ctggttatca gtactggtgc tggttccggc
    31021 gcgcagaacg cagtaggtac cggtgtaatg ggcgggatgg tgaccgcaac ggtactggca
    31081 atcttcttcg ttccggtatt ctttgtggtg gttcgccgcc gctttagccg caagaatgaa
    31141 gatatcgagc acagccatac tgtcgatcat cattgataca acgtgtaatc actaaggccg
    31201 cgtaagcggc cttttttatg cataacctac gaacattaag gagtaattga accaccaact
    31261 caggatctca tacgaaaacc agtattaacc acggataaaa ttcataaaaa atactgattg
    31321 ttagttaatt tatattaagt agcgctaata gatttaataa tccataatca tttagaggct
    31381 attcttaatt atttgcggta attctttatt cattcctcgg ttattacgtc atattcagag
    31441 caatcctggt attagtgtca ccaatttcat ctggcgataa tcctgaaatg ttatgaacag
    31501 ttcgagcaaa ctgcttttac ctgctgcggg ttggtgctag tatgaaaaag tgagccctgt
    31561 cccgcttcct tcctaattgt aatttttcgt aataatgcga tgaaaacctg caaagagtgg
    31621 cttatagtta agctaacaaa cgagagggca agtccaggtc agtaagtttt ttccatcccg
    31681 aaaggtgtcc gttagttcaa ccgctaagaa ggggacgcgt tatggatgaa tactcaccca
    31741 aaagacatga tatcgcacag cttaagtttc tctgtgaaac tctgtatcat gactgccttg
    31801 caaaccttga agaaagcaat catggctggg ttaacgaccc aacctcggcg atcaacctcc
    31861 agttgaatga actgattgag catattgcga ccttcgcact taattacaaa attaagtata
    31921 atgaagacaa taagctcatt gagcagatcg acgaatatct ggatgacacc tttatgttgt
    31981 tcagcagtta tggtattaat atgcaggatc ttcagaaatg gcggaagtca ggtaatcgac
    32041 tattccgttg ttttgtcaat gcgacgaaag agaatcctgc gagtttatct tgttagaatt
    32101 attacaacca tgggtagaag tatgtccgaa aaacctttaa cgaaaaccga ttatttaatg
    32161 cgtttacgtc gttgccagac aattgacacg ctggagcgtg ttatcgagaa aaataaatac
    32221 gaattatcag ataatgaact ggcggtattt tactcagccg cagatcaccg cctcgccgaa
    32281 ttgaccatga ataaactgta cgacaagatc ccttcctcag tatggaaatt tattcgctaa
    32341 taaataattc gctttcggag ctataaccgg ctgtttatta agaattttat actttttcgc
    32401 catgaagaca taccctatgt gatctttatc acacagatgt aatgggaacg ttctcttcac
    32461 tgacttttcg tcttactgtg ttgccgcatt ttcagcaacc ggaggtcagt aatgagcaca
    32521 gaaaaagaaa agatgattgc tggtgagttg tatcgctcgg cagatgagac gttatctcgc
    32581 gatcgcctgc gcgctcgtca gcttattcac cgatacaatc attccctggc ggaagagcac
    32641 acattacgcc agcaaattct cgctgattta ttcggtcagg tgacagaggc ttatattgag
    32701 ccaacgtttc gctgtgacta tggctataac atttttctcg gtaataattt tttcgccaac
    32761 ttcgattgcg tgatgcttga tgtctgccct attcgcatcg gtgataactg tatgttggca
    32821 ccaggcgttc atatctacac ggcaacacat cccatcgacc ctgtagcacg taatagcggt
    32881 gctgaactgg ggaaacccgt caccatcggt aataacgtct ggattggcgg acgcgcggtc
    32941 attaaccctg gtgtgaccat tggtgataac gtcgtggtag cctcaggtgc agttgtcaca
    33001 aaagatgtcc cggacaacgt tgtcgtgggc ggtaatccag ccagaataat taaaaaattg
    33061 taatcggttt ttcgcaactg ttatgcaaaa ttgtggtaaa tctgttactt tccctctact
    33121 attcccacgt taaaataggg tgttccctgg aaagttgcag ataccacgaa ggcaaacgat
    33181 gaccgaaata caacgcctgc tgaccgaaac gattgagtct ctgaataccc gcgaaaaacg
    33241 cgacaacaaa ccccgcttta gtatcagttt tatccgtaaa catccggggc tgtttatcgg
    33301 tatgtacgtt gctttttttg ccaccctggc ggtgatgttg cagtccgaaa cgctttcagg
    33361 ctctgtctgg ctactggttg tattatttat cctgcttaat ggtttcttct ttttcgatgt
    33421 ctacccacgc taccgctatg aagatatcga cgtgctggat ttccgcgttt gctataacgg
    33481 cgaatggtac aacacgcgct ttgtacctgc cgcgctggtt gaagccatct tgaactctcc
    33541 gcgtgtcgcg gatgttcata aggaacaact gcaaaaaatg atcgtccgta aaggtgaatt
    33601 gtctttttac gatattttta ccctcgctcg cgccgaatca acatcttaag ttaggattac
    33661 ataccaggcg taaagctctg cgcctggtca aatgacaacg atcgtttcca cccatcactt
    33721 catgaaatac cagctctacc tccttatctc ctgccagcct ttttccacaa tcagatatac
    33781 tttccctaca ctgtgttaat aaggatatgc tggtgagaac acgacatctg gtcggcctta
    33841 tttcgggagt actgattctt tcagtattgc tgcctgtcgg cttaagcatc tggctggccc
    33901 atcagcaggt agaaacatcg tttattgaag agctggatac ctattcctcc cgcgtcgcta
    33961 ttcgggccaa taaggtggcg acacaaggga aagatgcgct gcaagagctg gaaagatggc
    34021 aaggcgctga ctgtagcgaa gcccatctca tggaaatgcg tcgggtatct tacagttatc
    34081 gctatattca ggaagtggtt tatatcgata acaacgttcc ccaatgttcg tctctggagc
    34141 atgagagtcc gcccgatacc ttccccgagc caggtaaaat ttcgaaagat ggttatcgtg
    34201 tctggttaac atcgcataac gatttaggca ttatccgtta catggtcgcc atgggaacgg
    34261 cacattatgt cgtcatgatc gaccccgctt cctttattga tgtcattccc tatagctcat
    34321 ggcaaattga tgccgccatt attggcaatg cccataatgt tgtcataacc agcagcgatg
    34381 aaattgctca gggaattatt accaggctac aaaaaacacc cggtgagcat atcgaaaata
    34441 atggaatcat ttacgatatc ctgcccttcc cggagatgaa tatttcgatc atcacctggg
    34501 cttcaacgaa aatgttgcag aaaggctggc atcggcaagt ctttatttgg ttaccgctcg
    34561 ggttggtgat tggcctgctg gcagcgatgt ttgtgctgcg tattttgcgc cgcatccagt
    34621 caccgcatca tcggctgcag gatgctatcg aaaatcgtga tatttgcgtg cactatcagc
    34681 cgattgtctc cttagccaat ggcaaaattg tcggtgctga ggcactggcg cgctggccgc
    34741 agacagacgg cagttggttg tcaccagaca gttttattcc gctggcacag caaacgggcc
    34801 tttctgagcc attgacgcta ctgattataa gaagcgtctt tgaagatatg ggcgactggc
    34861 tgcgtcagca tccacagcag catatttcga tcaatcttga atccaccgtg ctcacctcgg
    34921 aaaaaatccc gcaattgctg cgtgaaatga tcaatcacta tcaggttaat cccagacaga
    34981 tcgcgcttga actcactgaa cgcgagtttg ccgatccgaa aaccagcgcc ccgataattt
    35041 ctcgctaccg ggaggcgggc catgaaattt atcttgatga ttttggtacg gggtattcaa
    35101 gtttaagtta tttacaggat ctggatgtcg acattctgaa gatcgataaa tctttcgttg
    35161 atgcgctgga atataaaaat gtcacgccgc atatcatcga aatggcaaaa acactgaaac
    35221 tgaaaatggt agcggaggga atcgaaacca gtaaacaaga agagtggcta cgccagcatg
    35281 gcgtgcacta cggtcagggc tggctctaca gcaaggcatt accgaaagaa gatttcttac
    35341 gctgggccga gcaacatttg tgaatttttg ggctgacggc acgccgcaca gcccgtcacg
    35401 ctacctattc gtcgatgatt gactcgaatc cgccatagat cattcgcttg ccgtcaaacg
    35461 gcatggactc gccgaactct ttcatccgtg ggtccgacat catcttttga ttagctgcgt
    35521 cgcggacctc ttttgaaggg tattcaatcc agctaaagac aacctcttca ttctcttccg
    35581 ctttcaccgc catacgaaaa tcggtcactt tgccatccgg tacatcgctg gcccagcatt
    35641 cgacaatacg aagcgcgcca aactctttaa acaatggcgc ggccttagcg gccatttccc
    35701 gataggcatc ctttttatcg gcaggaacgg caaccacaaa accatcaaca tacttcatca
    35761 gacatacctc cgaagcgtac tgcgcagcca gacaattctt gcgacgtcag ttgtggttaa
    35821 agtttagtca cgctgggatt ttttgacgaa acggtgggaa gcagaaatgg gtgggggccc
    35881 tgccagctac atcccggcac acgcgtcatc tgccttggct gcttccttcc ggacctgacc
    35941 tggtaaacag agtagcgttg cgggagaacc aacagagccc ccattgagag cgttgagaac
    36001 caacgcgcag gcgcattatc actgctgccc cggctaattg caacccattg cgtgccagat
    36061 gctggtttct tgcgcaatgc gacttcactc gggcgttttt ccagattatg ctgaaaaatt
    36121 acctcaggaa gaaaagatgg aaaaagaaga ttcatttccc caacgcgtct ggcaaatcgt
    36181 cgccgctatt cccgaaggct atgtaaccac ttacggtgat gtggcgaaac tggcgggatc
    36241 gccccgcgcc gcgcgccagg tgggcggtgt gttaaagcgt ctccctgaag gcagcacctt
    36301 accctggcac cgggtggtta atcgccacgg cacaatttcg ctaaccggac cggatttaca
    36361 gcgtcagcga caggcattac tggcagaagg tgtgatggta tcgggaagcg ggcaaatcga
    36421 cttgcagcgt tatcgctgga actactaacc ctctccgggc ggagagggtt aacgtcgctt
    36481 aatattgcgt aggagctgga actgccgaag acggattcac ctgagttggt gaagtcgaag
    36541 gtacggtagt cgttgcgcca ccgctggcct gaaccggcac ggcagtttgc tgtaccggca
    36601 ccaatgtcag gtcggcttta gttccgccct ggttgatcac cggctgaacg gtatcggtga
    36661 taaataccag tttgtcattc acggtaatcg ccgcactcaa cagaatacgc gcgttcggct
    36721 gaacatctgc cgggttaaat ggcagaacaa agctgaatgg tgactgttta ccttcagtac
    36781 gcaccgcttt ctgcgccagc acttttgacg gtgcatcggc taacgacgcg tcagaaagtg
    36841 tcacggtcag cacagcatca ggcggcagtg cgactttctg acggatccag acggtaccgg
    36901 agacattcgg ttgctggata gcaggttgtt gtgttgctga aatagacgta tttgcagccg
    36961 gggctggcgt ctgaatatcc gcgcttttat ctgcgcaagc cgccaacgca atcgcaaccg
    37021 ctaaaccact ggccatgtgc acgagtttca ttcatttctc cttattatca atgcaccagc
    37081 gggctaactt tcctcgccgg aagagtggtt aacaaaatag taacgtcaac aagtgtggca
    37141 cacatcacgc atttctgcct gtaattagcc cgtaattcag accattgcac ccatcggacc
    37201 agtagcaaat ttgcgttata ctcaactcac tttggcttgc tgcggcagct ttgttactgg
    37261 agagttatat gagtcaggcg ctaaaaaatt tactgacatt gttaaatctg gaaaaaattg
    37321 aggaaggact ctttcgcggc cagagtgaag atttaggttt acgccaggtg tttggcggcc
    37381 aggtcgtggg tcaggccttg tatgctgcaa aagagaccgt ccctgaagag cggctggtac
    37441 attcgtttca cagctacttt cttcgccctg gcgatagtaa gaagccgatt atttatgatg
    37501 tcgaaacgct gcgtgacggt aacagcttca gcgcccgccg ggttgctgct attcaaaacg
    37561 gcaaaccgat tttttatatg acagcctctt tccaggcacc agaagcgggt ttcgaacatc
    37621 aaaaaaaaat gccgtccgcg ccagcgcctg atggcctgcc ttcggaaacg caaatcgccc
    37681 aatcgctggc gcacctgctg ccgccagtgc tgaaagataa attcatctgc gatcgtccgc
    37741 tggaagtccg tccggtggag tttcataacc cactgaaagg tcacgtcgca gaaccacatc
    37801 gtcaggtgtg gatccgcgca aatggtagcg tgccggatga cctgcgcgtt catcagtatc
    37861 tgctcggtta cgcttctgat cttaacttcc tgccggtagc tctacagccg cacggcatcg
    37921 gttttctcga accggggatt cagattgcca ccattgacca ttccatgtgg ttccatcgcc
    37981 cgtttaattt gaatgaatgg ctgctgtata gcgtggagag cacctcggcg tccagcgcac
    38041 gcggctttgt gcgcggtgag ttttataccc aagacggcgt gctggttgcc tcgaccgttc
    38101 aggaaggggt gatgcgtaat cacaattaaa aaaatagccg gaggtgaaaa ccgtccggct
    38161 gttttttgca gtgcttgtta cgcgttatag gcattctcgc cgtgactgtt gacgtccagc
    38221 ccttctcgct cttgttcttc cggcacacgc aggccaaccg tcagatccgc cagtttgtag
    38281 ccaataaatg ccaccacacc ggaccagacg atcgtaatgg caatgctttc caactgtacc
    38341 agcaactgat ggcccatcgt cacaccttca gcgaagccca cgccgcccag cgaactggcg
    38401 gcaaaaatcc cggtcatgat acagccgaca atgccacaaa cgccgtgcac accgaagaca
    38461 tcgcagggat catccacccg cagcaagcgt ttgagcatgg taacgcccca caagcccgcc
    38521 agaccagcta ccacgccgat aatcaacgcg ccgccaaccc caatgtagcc acaggctggc
    38581 gtcacgccga ccagaccggc aatcgcgcca gaacacgccc ccagcagtga aggcttacca
    38641 cgcagtgccc attcaccgaa gatccagcca agaattgcag ccgccgttgc gaccacagta
    38701 ttcacaaaag ccagtgccgc gatttcattc gccgtgcccg ctgacccggc gttaaagcca
    38761 aaccaaccga tatagagaat ggcagtcccg gtgaagacca tcggcaggtt gtgtggttta
    38821 aacgcctctt taccgaagcc cacgcgtttt cctatcagat acgcgcccac cagaccggcg
    38881 attgcggcgt taatgtgcac caccgtgcca cccgcgaaat ccagcgcacc gtgagaagcc
    38941 agcaaaccac cgccccagac catatgcgca attggaatgt aagagagcgt cagccatacc
    39001 accacgaaaa tcaacacagc tgagaagcgg attcgttccg ccagcgcccc aactatcaag
    39061 cctacggtaa tgcaggcaaa cgatccctga aacgctacgt ggatatactg ataaatgctg
    39121 cccatcaccg ccgtcagttc gatgtttttc agcatcagcc agttaatgtt gccgaagaag
    39181 ttgttgccct caccaaacgc cagcgagtaa ccgtaaacca cccagagaat acagaccagt
    39241 gcaaacgtca ccgtcacctg cgtcagcatc gacagcacgt ttttgccgcg aatcaaccca
    39301 ccgtaaaaca gggcaatccc cggaatagtc ataaacagca ccagcgcagt acaaatcatc
    39361 ataaacgcat tgtcggcttt atcggccacc gcaggtgcag ctattaccag tcccggcagc
    39421 atcgccagtg aagcaagccc agtttttatc gtcgctatct tcatttttcg ttcctgttgc
    39481 tgtgtgccag agattacagc gccgcttcgt cggcttcgcc ggtacgaata cgaatgacgc
    39541 gttgcaattc agcgacgaag attttgccgt cgccaatttt tccggtgtaa gccgccttac
    39601 tgacgatatc gatcacttca tcgagttggt catcagcaat cgccacatca atttttactt
    39661 ttggcaggaa gttgacgctg tattccgccc cccggtacag ctcggcatgc cctttctgac
    39721 gcccgaaacc tttcacttcg gtgacggtca ggccctgaat accaatggaa gataacgctt
    39781 cacgaacgtc ttccagcttg aatggtttga ttatcacggt caccagcttc atagatcccc
    39841 tccggtcaga attcggtaat ggttctgcta cacgaagaag gtattgcaag cggtgtgcca
    39901 gaaatgaaaa acgagaaaga gagcaggaag ttaagaagat tgaaaatgaa aacgcactat
    39961 gacagtgcac agtgcgttac atttttgcac caacgatgaa atattgcgct attcaggcgc
    40021 tcaatgattc ttcttcacgc acgctggctg ccagctcttc gcccgcaagt tgcagttgat
    40081 acatctgcca gtagcgtccc tgggccgcca gcagttgctg gtgagtgccc tgctccacgg
    40141 cttgcccacg atgaagcacc agaatagtgt cggcatcaac aatggtcgat aagcggtgag
    40201 caatcaccac cagcgtggta tgttcacgca ccgccgccag agcatgttga atcgcctgtt
    40261 cagtaccgga gtcaatgctg gcggttgcct catcaaggat caggatttgc ggcgtctcca
    40321 ccagcacgcg cgccagtgcc agcagttgct tttgcccgac tgagagatta tttccctgct
    40381 cgcccagcgg cgtgtaaata ccgtcgctca tgctacgcgc cagctccgcc agttgcacgg
    40441 tttccagcgc ctgccagacg cgttcttcgg agatatcacg ccccagcgtc acgttggcga
    40501 ggaaggtatc cgccagcacc accggatctt gctgcaccat tgccacgccc tggcgcagcg
    40561 cgctgtgact tagcgaactt aatggacgac catcaaggcg aatctcacct tccgttagcg
    40621 ggtaatagcc catcaataaa ctggcgaggg tacttttacc actgccggta tgcccgacca
    40681 gcgccacaaa gttacgcgaa ggcacagaga gattaatgtt ctttagcacc agattgtcat
    40741 cgcgataagc aaatgacacg ttatcgactt cgatggtgcc gctctgtaac gggcgatcat
    40801 cattgccata ttgctggcgc ggtccgtcca tcagctcaaa cacgcgctca ccagcaacaa
    40861 cagcctgttg cagcatcgcc tgctgcgtgg tcagttcgat taagggttcg ttaagtcgcc
    40921 caagatagct gataaacgca tacaacacgc ccacttcaat ggtgccgctg gcggagaagc
    40981 caaacagcat caacaagcca cagagaatga gcgatgaaaa cagactcagc agcggacgca
    41041 ggagaaaacc gtcgaggcgc agggtttgca tcctcgccat atagtgtgaa cgactggcct
    41101 cacccatgcg ttcgccaaat cgcgcctgct ggcggaactg ctggataacg ctcatgccat
    41161 tgatgatttc gttaaagccg tcgttgatat ccgccagata ggcgcgcaca cgacggacaa
    41221 tcggcgtgct gtaacgctgg tatatcacca ttaccaccag caccaccggg aaaatcatta
    41281 tcgccaccag cgccattcgc cagtcgaggc tgaacatcgc caccagcatc gcgcccacca
    41341 gtgcggcact gcgcaggaca gttgccacta cggtaacgta gagatcgcgg atcacttcag
    41401 tgtcattagt gacgcgggaa atcacctgcc cgacgggttg ggtatcaaac tcgcttaatg
    41461 gctggcgtaa cgcagcatcc atcacgtcgg tacgcaactg ttgcactacg cctactgccg
    41521 cccgattaaa cagcagcgac tgcgcgtaat gtagcccggc ggcaaacagt tgtagcccaa
    41581 catacgccgc agccagccct gcaaccactt tcaacggcag gttatttttc gctaccatat
    41641 tgtcgataaa atagctgata agcagcggcc cactgacttc tgccgctgcc gcaacccaca
    41701 tcatcaggac cgcaatcccc agcggtttac gccacggcga accgtacgct aacagacgct
    41761 tgagagtcgg ccacagttgg ctaaaactac gcatcgacgg cctcctcgcg aatttccgga
    41821 gcgtcgtcga gcgccgcctc cagttgttga tagcgataca tatcgcgata ccagccactt
    41881 tgttgcgcca gctcatcatg attgccacgc tgggcgatat gtccgtgctg catcacaata
    41941 atttcactgg cttccgtcag tgcagaaagg cgatgggcac tgatgattac cgttcttccc
    42001 tgcccccact gacgcaggtt atgcaggatc tggtgctctg tgcgtccgtc caccgccgaa
    42061 agcgcatcat caaggatgag gatttccgcg ttgactaata acgcacgagc aatggagata
    42121 cgctgttttt gcccgccgga aagcatcaca ccgcgctcgc ccacctctgt atcgtaacct
    42181 tgcggtagac gcaaaatatc gtcatgtacg ctggctaacc gcgcgacatg ctcaatctct
    42241 tgctgggtgg cattcgggca acccagcgcg atgttattcg ccacggtgtc agaaaaaagg
    42301 aatggcgtct ggctaactac cgccaggcgg ctacgccagc tatcgagttg taacttcgtc
    42361 agaggaatat catgaaagcg aatatccccc tcgctgacgt cgaaatgacg ctgaatgagc
    42421 gacaacaggg tacttttgcc ggaaccagtc ggcccgcaga tacccagcat ctgaccgggt
    42481 ttcagggcga aattgacgtt ttccagcgca ggatggtcag tctgcggata cgtgaactgg
    42541 tgaatattta catccagttc gccacgccct tccggcacgg gttcgctacc atcgttcacc
    42601 accggcgctt ccgccagcat cgcgcgaata cggctgtacg cagcactacc acgttccaca
    42661 atgttaaaca tccatgccag cgccagcatt ggccaaatca tcagacctaa atacatcata
    42721 aaactggtga gctggcccag cgttaaactg ccctgcacca ccatccaact accgccgcca
    42781 atcgccagca agttcgccat accaatcgcg atatagatgg tcgggtcgaa acgagcatca
    42841 atacgcgcca cccgcatgtt ttttttgccg gtatcttcgg catccgcggc aaataacgcc
    42901 gactggcgat cttccagacc aaaggctttg atcatgcgga tactggtgag gctttcctgg
    42961 gtgcggtcat taagactgga aaacgccgcc tgtgccagct taaagcgttc atgcaaagca
    43021 tcgccgttgc gcttaatcat gatcgccatc actggcatcg gtaacagggc aaataaggtc
    43081 aactgccagc taatttgcgt agacatcata atcaacacag cgcagcccat taccagtgaa
    43141 tccaccagcg tcagcacccc ttctccggcg gcaaacacga cgcgatcgac gtcattagtc
    43201 gcacgagcca tgaggtcacc cgtgcgatga cgcaggtaaa actcaggatg ctgccggctt
    43261 agctgacggt aataatcttc acgcagttca acagccagtt gataagacgc accaaacagc
    43321 aatacgcgcc agacgtaacg caggagataa accacaacgg caatcagcac catggtggcg
    43381 atccacatca ggatctgccc ggtagtaaag tgttgttctg tcacgccatc gacaacaata
    43441 ccaaccactt ttggtggaac cagttgcagc atcgcgataa tgacaagcaa ggcgacagcc
    43501 ccgagatagc gacgccattc ccgacggaaa taccagctta attgagcaaa taatcgcacg
    43561 cgttgtgtcc tgacctgatt ctggatattt attcgatggg taaagaagtg gtgtatttaa
    43621 tctgttccat cgcgaagctg gaagtgacgt ccgacagccc cggcacgctg tttaccagac
    43681 gcttataaaa ctcgtcgtag cgtttcatgt cggcaacctg gacgcgcatc agataatcgt
    43741 attcaccagc catgcgccag aaccccagca cttctggcat ttcggtaacc accgtgacaa
    43801 agcggcaata ccattcgctg ctgtgatgtt gcgtttttat cagcacaaag gcggtcaggc
    43861 cgaggcctat tttttccgga tccagcaggg cgactttgcc gataaggata ccgtcgtcct
    43921 ccagccgttt caggcgcttc cagcaagggg tggttgtcag attaacggct tcagccagtg
    43981 cctgcaaaga gagggtgcaa tcctgctgca gtaaggccag cagcttacgg tcaattttat
    44041 ctaacatagc ccttccacag agaatttttt tctcttttaa ttctatttta aaggtgaaat
    44101 cgcgacaatt tattctgtgc ggtttaaaca ccacggcaca aaatgacaaa ttcaacgcaa
    44161 aaaaaagccg ggcaattgcc cggtgctgga aggaatctcg ttattcgggg gaataaggta
    44221 gatgtggata gtccagccag tgcgtcaaat agtgagagac agcctgattt cggcaatgtc
    44281 caatcaccgg taaatgcggg agctccgcgc gcagttgcgg catcgcattg cccataataa
    44341 atccgctacc gacgctgcct aacatttcgc gatcgttcat cgcatcacca aaggccatgc
    44401 aatcgcgcaa cgataaacct aaatgttggg tcagtaccgt caatgcagcg cctttattgc
    44461 agcccaccgg cagcacttca aggcaatccg tggcggaaaa acacaaatgt gcacgctcgc
    44521 ctaatgcttc gtatagctgg atctgcaaac gtgtaagatc gtcgtgatcg ccacagaagc
    44581 agatcttggt gacgctgccg agtggcattt ttttgacatc gattatttga taacgaaaac
    44641 cgctatagac aaatgcctgc aacaacgcag ggatctcttt cccggtaaac caaccgtcgt
    44701 cattgaagat atgcatgctg gctcgggtat cccattgctg atacagcacc agctccgcga
    44761 catccgcagg taaatcatca cgatgtaaaa gttcaccttc cagagaatgc acgcgcgttc
    44821 cgttgccggt aatcaaatac gcatccagcg atagcgcccc gagaatatgc tgcatctcca
    44881 gcgcatgacg ccccgtggca aaagtgaggg taatgtcgcg ttcacgcagt cgcgccaaag
    44941 tagagagggt tttctcacct aaatgatgat caggcatcaa taaagtgcca tccatatcaa
    45001 atgctgccag acgagccatt tctttctcca ctctgtgaca cggttaatgg tgattgagta
    45061 tcacctgata tatacggaag taatagtgaa tagttaaata atattgttcc gggtttatat
    45121 gcgactgctc aaccgtctta accagtatca acgtctgtgg caaccttccg ccggaaagcc
    45181 gcaaaccgtc accgtcagcg aactggccga acgctgtttt tgcagcgaac gccatgttcg
    45241 tacgctgttg cgtcaggcac aggaggcggg atggctggag tggcaggcgc agtcaggacg
    45301 cggaaagcgc ggacaattac gctttctggt cacgccagaa tcgctacgca atgcgatgat
    45361 ggaaaaggca ctggaaaccg gaaagcagca agatgtgctg gagctggcgc aactggcccc
    45421 aggtgagctg cgcactctgt tacagccgtt tatgggcgga caatggcaaa acgatacgcc
    45481 cacgttgcgt attccctact atcgcccgct cgaaccgcta caaccgggct ttttgcccgg
    45541 ccgtgccgag cagcatctcg ccggacagat attttccggc ctgacccgct tcgataataa
    45601 tacccagcgc ccgattggcg atttagcgca tcactgggaa acctctgctg acgggttacg
    45661 ctgggacttt tatcttcgtt caaccctaca ctggcataac ggcgatgcag taaaagcctc
    45721 acacttacac cagcgattat tgatgctgtt acaactgcca gcactggatc aattatttat
    45781 tagcgtgaag cgtattgagg tcacccatcc gcagtgtctg accttctttt tacatcgccc
    45841 tgattactgg cttgcgcacc ggctggcgag ctattgcagc catctggcgc atccgcaatt
    45901 cccactgatc ggcacgggtc cttttcgctt aacacaattc acagcggagc tggtgcgcct
    45961 ggaaagccat gattattacc atttacgtca tccgctgctt aaagcggttg agtactggat
    46021 aactccgccg cttttcgaaa aagatttggg aaccagttgt cggcatcccg tgcaaatcac
    46081 catcggcaaa ccggaggagc tgcaacgggt cagccaggtc agtagtggca tcagtttagg
    46141 tttttgctat ttaacgttgc gcaaaagtcc ccgactctcc ctctggcagg cgcgaaaagt
    46201 aatctccatt attcatcaat ccggtttatt acaaacgtta gaagtcggag aaaacctgat
    46261 caccgccagt catgcattac tgccaggctg gactattccg cattggcaag taccggatga
    46321 agtcaaacta ccgaaaacct tgacgctggt ttatcaccta ccgatagaac ttcataccat
    46381 ggcagaacgc ctacaggcga cactggcagc ggaaggctgt gaactcacaa ttatttttca
    46441 taacgcaaaa aactgggacg acacgaccct actggcacac gcagacctca tgatgggcga
    46501 cagattaatt ggcgaagcac cggaatatac tctggagcaa tggctacgtt gcgatccact
    46561 gtggccacat gttttcgacg ctccagcata tgcccatttg caatcgacac tggacgcggt
    46621 gcaagtaatg cctgatgagg aaaaccgatt taatgccctg aaagcggttt ttagccagtt
    46681 aatggcagac gcgacgctga cgccgctatt caactatcac tatcgcatta gtgcccctcc
    46741 cggcgtgaac ggtgtgcgac tgacaccgcg cggctggttt gaatttaccg aagcctggct
    46801 tcccgcgccg tcgcaatgaa tagctggtcc gggttaatcg caggcgttac cataacgctt
    46861 ttattgttca agcaggatta tctatgaaac gtgctgtcgt tgtgttcagt ggaggtcagg
    46921 attccaccac ctgtctggtg caggcattac aacaatatga tgaagtccat tgcgtgacgt
    46981 tcgattacgg tcagcgccat cgcgcagaaa tcgacgtggc acgcgaactg gcgctgaaac
    47041 tgggggcacg cgcgcataag gttcttgatg taacgctgct caacgagctg gcggtcagca
    47101 gcctgacgcg tgacagcatt ccggtgcctg attatgaacc tgaagccgat ggcatcccga
    47161 atacgtttgt cccagggcgt aatattttgt tcctgacact ggcggcaata tatgcgtatc
    47221 aggtaaaagc agaagccgta attactggcg tctgcgaaac ggatttctcc ggctacccgg
    47281 attgccgcga tgagtttgtg aaagcactaa accatgccgt cagtttgggc atggcgaaag
    47341 atattcgttt tgaaacgccg ctgatgtgga ttgataaagc ggaaacctgg gcgctggcag
    47401 attattacgg caaactggat ttagtccgta acgaaacgtt gacctgctat aacggcatta
    47461 aaggcgacgg ttgcggtcat tgtgcggcgt gtaatttacg cgccaacggt ttgaaccatt
    47521 atctgaccga taaaccgacg gtgatggcag cgatgaagca gaaaacaggg ttgaagtaat
    47581 tattccaagt attgccggat gcggcttgag catccggcac aacaaaacgt ttacttaacc
    47641 atctgctcca gcttttcgcg caattcccct tccagagcta atgctttctg cgttttaaga
    47701 tcaatacaaa caaacgtaat aagcgcatcc gctactacct gcccttccgg ctccagtgta
    47761 atgacctggc ttaagatgcc gcttttaccg tttaattgct gtaactggct cgtaatggtt
    47821 aacaagtcgc ttaataccgc cgggcgacga tagttaatat tgatattcac caccacgaaa
    47881 gcgatgttat gggccgtcat ccagtgaaaa ctgtcgctat tttccaaccc atcccagcgg
    47941 gcttcttcga gaaattcaag gtagcgggcg ttgtttacgt gctggtaaac gtcgagatga
    48001 tatccacgaa ctttgatttg tgtttgcata gcgcaataac cttacgttgt ttttataaaa
    48061 actgttataa gtacagaggt cataactggt atgacctctt cagtctggca aaaaattgcc
    48121 actatgcaaa ttaattacag ggttaatacc gccagattac gttccaccag cgaattgccc
    48181 atccccggca cctgctttag atcctccacc gttttaaacg gaccgtactc ttcgcgataa
    48241 ctgacaatcg cctgcgcttt cttcaggcca acgccattca tcgcgcgggc tagctcttcc
    48301 gcgctggcat tattaatgct gacccgggtg ccttcttcgt cactggcttt cgccggtact
    48361 gctgctttac tttgtgctgc aggagcttcc gctttggttt ctaccgccgt cggtttcgcc
    48421 acagaagctg ccgccagcgc gctatgagac attccggcac aggccaggga cagggtaatg
    48481 agcagtgctt taattccgtg tttcatactg ttttctcctt gtttgctaac agtggaatca
    48541 ccttaacggg agtgaaaaca gtaaacaaat ggcaatgttc agaaatggaa aaggccgcga
    48601 aagcgacctt ttcttgttac agctgcaaag catttttttg cgaggcggct tcaggattat
    48661 tgctgttcca gcgcatcgcc aattttgatt ttcgcctctt tacgcaggtt actcatcaga
    48721 gcttcaaaga cgatttgtgc gttgttctgg gtgataccct gcaccatcgc ttttttctga
    48781 tcttccggca ttgaaccttg tttcacttca tccagcgcca gcagaaccac attaccttgc
    48841 atatcggtcg ccataccgta gctcggtttg tctttcgctg gcagtggcag tgcaaacgcc
    48901 gcctggctaa tcgggtcacg accggaacgg cttaaggttt tcggctctcc aaatttcaga
    48961 ccggcagcct gcatagcttc cgcacctttg ccggctttca aatcaaccag cagtttctca
    49021 gcatccactt tcgcctgttg ttcagctttg ttgtgctgaa ccagtgcctt aacttgttcc
    49081 tgaacatctg ccaacggttt caccgcttcc ggtttgtgct cgctgatgcg cagcacgaat
    49141 gcgcggtcgc cgtctacggt gatgatgtca gagttgatgc ccggcgcgcc gttttcacct
    49201 accagaccgc cgttaaagat agcgtcggca accggcttga agttcaactc ttccggcagg
    49261 ttatctttgc tgaaccaacc cgtctgagtg gctttaacgc cggcagcttg ctctgcaccg
    49321 gccagagact cggtgtcgtt gcttgccgca tcgctcactt tctgctgcag cgcgtagtac
    49381 gcatcgaggg ctttttcgtg tttcactttc gccgcaatgt cgtcacgtac tttgtctaac
    49441 gatttcactt tcgctggctg aatgtcgtcc agacgtacaa tcaggaaacc gaccgaagat
    49501 ttgatgacac cagacagttg gcctttttct ttcagaccag catttttcag ttcatccggg
    49561 atagtggcat cttctaacca acccatatcg ccgccgttac gagcagagat aatatcggca
    49621 gatttttctt tggctaatgc agcaaaatca ccgcctttat tcagctcatc aagtaccgct
    49681 ttcgcttcat cttcagtttt ggtctggatg atgctgtagc gggtacgctg cggctgggtg
    49741 aattgatcct gatgctggtc gtagtagctc tggatatccg catcgctaac cggttgctgc
    49801 atcgttgcgg catccagctt gatgtaactc acgcggaatt gttccggcgt catgaaattg
    49861 tttttgtttt gttcgtagta gctggcaatc tcctgttcgg tcacaggctg cttcgccgcc
    49921 agcgcgttaa catcgatagt cgcttcacgt accacgcgtt gttgagcgac tagtgccgcc
    49981 agctcgtcgg tttcaccttt cagcataaaa tcggtaccgg caacgccgtt aatcagctgt
    50041 tgggtagtga gctggttacg cagcgcctgg gcgtactgat cggcggtcat ccccatctgg
    50101 ttgaggatac cgttatagcg gctgttatca aatttgccgt caacctggaa ggctggggtc
    50161 gcgaaaatcg cctgtttaac ctgctcatcg ctgataccca gtttcagctc acgtgcgtac
    50221 tgatccagca gcgcctcgtc gatcagacga ttcagcacct gttgacgcag ggttttcata
    50281 tagccttcgt tcgctgccag ctcggagtat tgatcgccca gctgttgctg catgcgatta
    50341 cgctcgctgt tgaaggcatt ctcgaactgc ccacggctga tttcctggtc attcactttt
    50401 gcggcgtagt tattgcctcc gccaatcagg taaccactca cgccggtcaa tatgaacgac
    50461 acgataatga taccgaaaat aatcttgagc acgagactgt ttgcagccgt gcgtaagctg
    50521 tccatcatgg tgtaacaaca ctccgctgta gatgactgtg tacctcacgc aacgtatcga
    50581 tgcgcgcaaa ggcctattgt gacaagaaac gggggcaatt gtcagcccac aagacacaga
    50641 aagtcaccga atacaaataa aaaaggcaca tcagtagatg cgcccttgaa cttcgtcaca
    50701 tccccactgg ggacaacgct tagtttaccg cgtctttcag tgctttacct gcacggaagc
    50761 tcggtacttt agcggcagcg atggtgatct ctttaccggt ctgcgggttg cggccagtac
    50821 gggcagcacg ctctttaacg gcaaaagtac caaaacctac cagtgctaca tcatcccctt
    50881 ctttcagaga ttcagttacg gaagcaataa tagcatctaa cgcacggcca gccgcagctt
    50941 tagagatatc agcccctgca gcaatcttgt cgatcaattg agatttattc actcttctct
    51001 tcctctttat aatttatatc gcacttgaat ccttcaaggt acgaacgcgc agcagttata
    51061 tcaggccagc catcccctta caacacccct taataagggc aagcccgatc cgccatctaa
    51121 cttagcgaga caaaaaaagg ctggcaagcc cgaattagcc tgccagccct gtttttatta
    51181 gtgcattttg cgcgaggtca ctattttgca gtcacaacct gcatgccaga cggttcattt
    51241 tgcagcgcca gagtcagaac ttcctcaatg cgcttcacag gatgaatgtc cagatcggca
    51301 attacgttgt caggaatctc ttccagatcg cgtttatttt cgaacggaat taacactgtt
    51361 ttaatcccac cgcgatgcgc tgccagtagt ttttctttca aaccaccgat cggcagtacc
    51421 tgaccacgca gagtgatctc accggtcatt gccacatcgg cacgaaccgg gttaccggtc
    51481 aggcaagaaa ccagcgcggt gcacatagca ataccagcac tcggaccatc tttcggcgtc
    51541 gcaccttccg gtacgtggac gtggatgtcg cgtttttcgt aaaaatcagg gttgatcccc
    51601 agtttttccg cacgcgcacg aaccaccgtt aacgccgcct gaatggactc ctgcatcact
    51661 tcgccgagcg aaccggtata ggtcagtttg cctttccccg gaacgcacgc ggtttcaatg
    51721 gtcagcaagt caccgcccac ttccgtccac gccagaccgg ttacctggcc gacacggttt
    51781 tcgttatcag cgcgtccata gtcgaaacgc tgaacaccga gatagtcatg caagttatcg
    51841 ccgttaattt cgatatgttt taatgactta tcgagcagta actgcttaac cgctttacgg
    51901 cacagtttgg agatttcacg ctccagacca cgcacgcccg cctcacgggt gtagtaacga
    51961 ataatgccga taatggcgct atcgtcgacg gtcagctcac cttttttcag tgcattacgt
    52021 tcaatctgct tcggcagcag gtgacgtttg gcgatgttca gtttttcatc ttcggtataa
    52081 ccggagaggc gaatcacttc catacgatcc agcagcggtg ccggaatgtt catggagttc
    52141 gacgtcgcga caaacatcac gtcgctgaga tcgtaatcca cttccaggta gtggtcgctg
    52201 aacgctacgt tctgctctgg atccagcact tcaagcagtg cagaggccgg atcgcctcgc
    52261 atgtcagaag acattttgtc gatctcatcg agcaggaaca gcgggttttt cacgcccact
    52321 ttcgccattt tctggatcag tttacccggc atagaaccga tgtaagtacg gcggtgacca
    52381 cggatttccg cttcatcacg cacgccgccc agcgccatac ggacatattt acgcccggtg
    52441 gctttggcga tggactgacc cagggaggtt ttacctactc ccggcggccc taccaggcag
    52501 aggatcggtc ccttgatttt gttgacacgg ctttgaaccg caagatactc aaggattcga
    52561 tctttcacgc gctccagacc ataatggtcg gtatcaagga tttcctgcgc ctgacgcagg
    52621 tcttttttga ccttgctgcg cgcattccac ggtacctgta ccatccagtc gatataacca
    52681 cgcactacgg tcgcttctgc cgacatcgga gacatcattt tcagcttctg caactctgct
    52741 tccgctttct cttttgcctc tttcggcatc ttcgccgcgt cgattttgcg cttcagggct
    52801 tcgttttcgt ccggcgcgtc gtccatttca ccgagttctt tctgaatagc tttcatttgc
    52861 tcgttcagat agtactcacg ctgggatttc tccatctgct ttttaacgcg gttgcgaatg
    52921 cgtttctcaa cctgcagcag atcgatttcc gattccatca ttgccatcag atattccaga
    52981 cgttcgttaa cgtcggacat ctccagaacg gactgtttgt cagccagttt cagcggcata
    53041 tgtgcagcaa tggtatccgc cagacgcgcc ggatcgtcga tgctattcag cgacgtcagc
    53101 acttctggtg ggattttttt gttcagcttg atgtagcctt cgaactggct gattgcagta
    53161 cgcaccagca cttcctgttc tcgctcatca atggtcggcg actccagata ctccgccttc
    53221 gcagaaaagt gttcgccatt gtcagagagc gcagaaatac gcgcgcgctg taacccctcg
    53281 accagcactt tgacggtgcc gtcaggcagt ttcagcatct gcaatataga ggccacggtc
    53341 ccgacggtga aaagatcgtt tacacccggc tcatccgttg aagcttcttt ctgcgcgacc
    53401 agcataattt ttttatcatg gtccatcgcc gcttccagac aacggataga tttttcccgc
    53461 ccgacaaata aggggatgac catgtgcgga taaaccacca catcgcgcag cggcaatacg
    53521 gggatttcaa tgcgttcaga acgctcagga ttcatagagc tctctcttag tttaatttcc
    53581 gccaggtaat cagatgacac gactgtgctt cacgccatct attaacatgt acgtcagtat
    53641 atggggatgt ttcccccaca ttcaacgccg agaatagagg aaaaattaaa ggggagataa
    53701 aatcccccct ttttggttaa ctaattgtat gggaatggtt aattattcac cagatgcctg
    53761 ttgcgcttcc ggcttgccat aaatcagcaa cggtttgctt tgaccatcaa ttaccgactc
    53821 gtcgataacc actttttcga catcttccat ggacggcaga tcgtacatgg tatcgagcag
    53881 tgcggcttct acgatggaac gcaggccacg ggcaccggtt ttacgcgcca tcgctttctt
    53941 agcgatagca tccagcgcct cgtcacggaa ttccagatcc acgccttcca gattaaacag
    54001 cgcctgatac tgcttggtca gggcgttttt cggctctttg aggatctgaa tcagagcttc
    54061 ttcgctcagt tcattcaacg ttgcgacaac cggcagacga ccaataaact cagggataag
    54121 accaaacttg atcagatctt ccggttcaac ctgcgccagc agctcgcctt cgcttgcttt
    54181 gtcggacttc gcttttaccg tcgcgccaaa accaatgccg gagccggttt ctacacgatg
    54241 ggaaatcact ttatccagac cggcaaacgc accgccacaa ataaacagga tcttagaggt
    54301 atcaacctgc aagaattcct gctgcggatg tttacgccca ccttgcggtg gaacagcagc
    54361 taccgtacct tcgatcagtt tcaacagtgc ctgctgtacg ccttcaccgg aaacgtcacg
    54421 ggtaatagac gggttgtctg acttacgaga aatcttgtcg atttcatcga tgtagacaat
    54481 cccgcgctgc gctttctgta cgtcgtaatc gcacttttgc aacagcttct gaatgatgtt
    54541 ttcaacgtct tcgcccacat aaccggcttc ggtcagcgtg gttgcgtcgg ccatggtgaa
    54601 cggaacgtcc agcaggcgcg ccagcgtttc agccagcagc gttttaccgg aaccggtcgg
    54661 accgatcagc agaatgttac ttttgcccaa ctcgacgcca ttgctggtat cgccgttgcg
    54721 cagacgtttg tagtggttgt ataccgcgac cgccagcact tttttcgcct gttcctggcc
    54781 gataacgtaa tcgtccaggt ggttacgaat ttcatgcggc gtcggtagcg cactgcgttc
    54841 acgatgcggt gcaacttctt taatctcttc gcgaatgatg tcgttacata aatcaacaca
    54901 ttcgtcgcag atatacacgg atggaccggc aatcagcttg cgcacttcat gctggctttt
    54961 gccgcaaaaa gagcaataca gcaatttgcc tgagccatct ttgcgtttat ctgtcatggg
    55021 tcaaaacctc ttctttgttc tttgtgccgc acacgacgac gcaaatgcca ttctcaggcg
    55081 caagccgctt acagcgttgt gccgccctgg ataagtatag cggcacagtt gcgcctctgg
    55141 catcaattac gatgggtcag aatcgaatcg accagaccgt attccaccgc ttcaggggcg
    55201 gaaaggaagc gatcgcgctc ggtatcacgt tcaatctgtt ctaatgattt acccgtatga
    55261 agcgccataa gttcattcat acgcccttta actttcagaa tttcacgggc atgaatttcg
    55321 atatcggtcg cctggccctg gtagccgccc aacggttggt gaatcatcac gcgcgaattc
    55381 ggcaggcaaa aacgtttacc ttttgcccct gcggtcagca agaaagcgcc catcgaggcc
    55441 gcctggccca tacagatggt gctgacatca ggcttgataa actgcatggt gtcatagata
    55501 gacatcccgg cagtgatcac cccgcctggg gagttaatgt acagatagat atctttttct
    55561 gggttttctg cttccaggaa cagcatctgc gccacaatca ggttagccat gtggtcttca
    55621 acctggccag tcagaaaaat gacgcgttcc ttaagtagac gagaatagat atcaaaagag
    55681 cgctcaccac gtgaggtctg ttcaatgacc atcggcacca gcgccatatg gggtgcaaag
    55741 ttatctcgtt cgccgctgta tgacatttcc gtctcctgga taaaattgaa aaaacctgct
    55801 gtaccgattg taaccttatg gacggattat cagctagtcc ctgtgttatg ggtacggcat
    55861 caccatattt caagcataac aatcttttgt tgttacgcta acaccgaaag aggcttttcg
    55921 cacggttcca tgcaaaattc atgacaaaaa aaagcccacc acctggcggt gacgggcctt
    55981 tgtgcgaatt tagcgcgtta tgctgcgtaa attacgcctg ctggttcatc agctcgttga
    56041 aagtggtttc tttttcagtc actttcgctt tcgccagtac agcttcaaca gcctgttctt
    56101 ccagagcaac attgcgcatg ttgtccatca gttctttgtt tttgctgtag aactcgataa
    56161 cttctttcgg atcttcgtac gcagaagcca tctcttcgat caggcctttc acgcgctctt
    56221 cgtcagcttt cagctcgttg gtgcggataa cttcgcccag cagcaggcca acaactacgc
    56281 ggcgtttagc ctgttcttcg aacagttcgc gcggcagttc cagagcttgt ttttcgttgc
    56341 caccgaaacg ctgtgcagcc tggcgacgca gaacgtcgat ttcgctgtca atcagcgcag
    56401 ccggtacgtc gatgtcgtta gcttttacca gaccttcgat cgcctgagac ttaacgcggt
    56461 tacggatggc gctcttcagc tcgcgctcca tgtttttacg cacttcagcg cgcagacctt
    56521 ctacggaacc atcttcaacg ccgaaacgtt tgatgaattc tgcagtcagt tccggcagtt
    56581 cacgctcttc aactttcttc aggttgatag cgaatttcgc tgctttacct ttcaggtttt
    56641 ctgcgtggta ttcttccggg aaggtcacgt cgatggagaa ctcttcgcca gctttgtggc
    56701 ctttgatacc gtcttcaaag cccgggatca tacgaccctg gcccatcgcc agtacgaaat
    56761 cagacgcttt accgccttcg aactcttcgc cgtctacaga accggtgaag tcgatggtta
    56821 cgcggtcttc tgcttcaaca gcgccgtctt tttctttcca ggtcgcctgc tgtttacgca
    56881 gagtatccag catgccgtca acgtcagcgt cggtcacttc aacgatcggt ttttcaactt
    56941 cgatcgcttc cagaccctgc agttcaactt ccggataaac ttcaaactct acagagtagg
    57001 tgaagtcttc acccagcttg tattcgcccg gaacataagt cggtgcgcca gccggattga
    57061 ttttttcttt aatgatggcg tcaatgaagt tacggctcat caggtcaccc agaacgtcct
    57121 ggcgtacaga cgcgccataa cgctgagcaa cgatattcat tggcactttg cctttgcgga
    57181 agccgtcaat acgtactttt tttgcaacgt tgaccagctc gcttttaaca gcggtctcga
    57241 tgctgtcagc agcgatagta atcgttacac ggcggccaag gccttgagtg gtttcaactg
    57301 aaacttgcat cttgttacct caaaaaatca cagtgctcgg tcaactctta ccccgcaagc
    57361 acaggttgtt ggcttcgcgg aaccgggatg ttcttataat caatcacatt ccctgttacc
    57421 agaatcatcc cgaaagcatt caaataggac gcggcattat agcggcatca cttttatgag
    57481 tcgagaacgg ttattgcgcg ctgtcgcgta ttttttcaca attcccgggc aattttcgtc
    57541 tgaatgctgg gcaaaaagcg caaaatccag acaaaacaaa acggcccgca ggccgttttt
    57601 cataaaatct ttagcaacat actggaaaag cgccgacagt tgcaaatgcg tttttacgcg
    57661 atgcttcctg ctccacgaca gggaggagag gcaaagacgg tgtcctgcaa cccttcccac
    57721 tccttaatag tgtaagtatg cagagccagc gcatgaacgg tagtagagag ttcctccgct
    57781 aaagtactgt aaatcattcg atgacgattc agaaaacgtt cacccgtaaa acgatcgctg
    57841 accagcacaa ctttaaaatg gctttcagag ccggctggga cattgtgacg atagctttca
    57901 tccactactt cgaggaatac gggttggaac gccgccctta atttttcttc tatccgctca
    57961 cgtatcatca tgaaatatct cctccgacaa cgctgagatg tcatccatcc ctttaaatac
    58021 tagccgcttt taccgtttcc attgcagctt aacaacaaat atttaggttt cgtcctggtt
    58081 tttccgtcaa gcgtatgaag tttactgatt taaagattga aacacaggcc tcgcatcagc
    58141 gtttctttta ccaggggcga ttatttcgtc aacatgatga atttacatgc cttacctact
    58201 tcccctcgcc gttggcaatg ttatgatggc gggaattttt accaaacacg cttaagagtc
    58261 tctgagagcc cgaacatgtt caaaaaaatc ctcttcccgt tagttgctct gtttatgctt
    58321 gcaggatgcg caaaaccgcc aacaactatt gaagtttccc cgacgattac actgccacag
    58381 caggatccaa gcctgatggg cgtcaccgta agcattaatg gtgccgatca gcgtaccgat
    58441 caggcgctgg caaaagtcac ccgcgataat caaatcgtta ccctgaccgc ctcccgcgat
    58501 ctgcgtttcc tgctgcaaga agtgctggaa aaacagatga ccgcgcgtgg ttacatggtt
    58561 ggtccgaatg gcccggttaa tctgcaaatc atcgttagcc aactgtatgc tgacgtgtcc
    58621 cagggcaacg tgcgctacaa catcgctacc aaagcggata tcgccatcat cgctaccgcg
    58681 cagaatggca acaaaatgac caaaaactat cgtgccagct acaacgttga aggtgcgttc
    58741 caggcctcca acaaaaatat cgccgatgcg gttaacagtg tgctgaccga taccatcgct
    58801 gatatgtctc aggacaccag cattcacgaa ttcatcaagc agaacgcgcg ttaatttctg
    58861 ccctctggcc cggtgcaagc cgggcctgta gacgcccatg tccagtcaat atttacgtat
    58921 ttttcaacag ccgcgttcag ccatattgct gatcctgggt tttgcttccg ggctaccgct
    58981 cgccctgaca tccggcacct tacaggcctg gatgacggtc gagaatatcg atctcaaaac
    59041 cattggtttc ttctctctgg taggccaggc ttacgtcttt aaattcctct ggtcaccgct
    59101 gatggaccgc tacacgcccc cattttttgg gcggcggcgc ggttggctgc tcgccacgca
    59161 aatcctgtta ttagtcgcca ttgcggcgat gggttttctc gaaccaggca cccaactccg
    59221 ctggatggcg gcgctggcgg tggtgatcgc tttttgctct gcctcccagg atattgtctt
    59281 cgatgcgtgg aaaaccgatg tgctcccggc agaagaacgt ggtgcgggcg cggcaatcag
    59341 cgtgctgggt taccgtttag ggatgttggt ttccggcggc ctagccctgt ggctggcaga
    59401 taaatggctg ggctggcagg gcatgtactg gttgatggcg gcgctgttga tcccctgtat
    59461 tatcgcgacg ttgcttgcac cagaaccaac cgacaccatt cctgtgccca aaacgctgga
    59521 acaagcggtt gttgcacctc tgcgagattt ctttggtcgc aataatgcct ggcttatttt
    59581 gcttcttatc gtgctgtata agctgggcga cgcattcgcc atgagcctga caaccacgtt
    59641 tttgattcgc ggcgtcgggt ttgatgcggg tgaagtaggc gtggttaaca aaacactggg
    59701 cttactggcg accattgttg gcgcattgta cggtgggatt ttgatgcagc gcctgtcact
    59761 gttccgggca ctgctgattt tcggcatttt acaaggtgcg tctaacgctg gttactggct
    59821 gctgtcgatt actgataagc atctctacag catgggcgca gccgtctttt tcgaaaacct
    59881 ctgtggcggg atgggcacat cagcctttgt cgcgctgtta atgacgctat gtaataagtc
    59941 attttccgct actcaatttg ccctgctctc agcgctttct gctgtagggc gagtttatgt
    60001 cggccccgtg gcgggttggt ttgttgaagc acacggctgg tcgacattct atctattctc
    60061 cgtcgccgct gccgtaccag ggcttatttt gctgctggtt tgccgccaga cgcttgaata
    60121 tacacgagta aatgacaact ttatctcccg taccgcatat ccggcaggtt atgcctttgc
    60181 catgtggaca ctggcggcgg gcgtcagcct gttggccgtg tggttactgc tgttgacgat
    60241 ggacgcgctg gatttgacgc acttctcttt cctgcctgct ctgctggaag tcggggtttt
    60301 agtcgccctt tctggcgtcg tgcttggtgg tttgctggat tatctggcgc tacgaaaaac
    60361 gcatctgacg taatctgtaa atattattta gctgtagtaa tcactcgccg taattattac
    60421 ggctaaataa acatcagcat cgcttattaa tatttgttct tttgtgcggc ttagcgtttg
    60481 gaaattattg gcgccattta taaattctat ttttcttaca cgattcagct aatgagtctt
    60541 tattttctca cccccagttg tcactccaat gataattatt tgttaaataa ttgttttatt
    60601 tcgtattggt tataccaatt gcccgcccag actccttctg cactcccctt ttgttataac
    60661 gcccttttgc aacagcttct taaaatcaac ctgatatgtt ttgcaacata tgtgacctga
    60721 cagccaaatc caagtaacag gaatttaatc atgtttacag taatgtaacc ttcccgtaaa
    60781 atgcccacac actttaaacg ccaccagatc ccgtggaatt gaggtcgtta aatgagactc
    60841 aggaaataca ataaaagttt gggatggttg tcattatttg caggcactgt attgctcagt
    60901 ggctgtaatt ctgcgctgtt agatcccaaa ggacagattg gtctggagca acgttcactg
    60961 atactgacgg catttggcct gatgttgatt gtcgttattc ccgcaatctt gatggctgtt
    61021 ggtttcgcct ggaagtaccg tgcgagcaat aaagatgcta agtacagccc gaactggtca
    61081 cactccaata aagtggaagc tgtggtctgg acggtaccta tcttaatcat catcttcctt
    61141 gcggtactga cctggaaaac cactcacgct cttgagccta gcaagccgct ggcacacgac
    61201 gagaagccca ttaccatcga agtggtttcc atggactgga aatggttctt catctacccg
    61261 gaacagggca ttgctaccgt gaatgaaatc gctttcccgg cgaacactcc ggtgtacttc
    61321 aaagtgacct ccaactccgt gatgaactcc ttcttcattc cgcgtctggg tagccagatt
    61381 tatgccatgg ccggtatgca gactcgcctg catctgatcg ccaacgaacc cggcacttat
    61441 gacggtatct ccgccagcta cagcggcccg ggcttctcag gcatgaagtt caaagctatt
    61501 gcaacaccgg atcgcgccgc attcgaccag tgggtcgcaa aagcgaaaca gtcgccgaac
    61561 tccatgtctg acatggcagc gttcgaaaaa ctggccgcgc ctagcgaata caaccaggtg
    61621 gaatatttct ccaacgtgaa accagacttg tttgccgatg taattaacaa gtttatggct
    61681 cacggtaaga gcatggacat gacccagcca gaaggtgagc acagcgcaca cgaaggtatg
    61741 gaaggcatgg acatgagcca cgcggaatcc gcccattaaa ggggttgagg aagaataaag
    61801 atgttcggaa aattatcact tgatgcagtc ccgttccatg aacctatcgt catggttacg
    61861 atcgctggca ttattttggg aggtctggcg ctcgttggcc tgatcactta cttcggtaag
    61921 tggacctacc tgtggaaaga gtggctgacc tccgtcgacc ataaacgcct cggtatcatg
    61981 tatatcatcg tggcgattgt gatgttgctg cgtggttttg ctgacgccat tatgatgcgt
    62041 agccagcagg ctcttgcctc ggcgggcgaa gcgggcttcc tgccacctca ccactacgat
    62101 cagatcttta ccgcgcacgg cgtgattatg atcttcttcg tggcgatgcc tttcgttatc
    62161 ggtctgatga acctggtggt tccgctgcag atcggcgcgc gtgacgttgc gttcccgttc
    62221 ctcaacaact taagcttctg gtttactgtt gttggtgtga ttctggtaaa cgtttctctc
    62281 ggcgtgggcg aatttgcgca gaccggctgg ctggcctatc caccgctatc gggaatagag
    62341 tacagtccgg gagtcggtgt cgattactgg atatggagtc tccagctatc cggtataggt
    62401 acgacgctta ccggtatcaa cttcttcgtt accattctga agatgcgcgc accgggcatg
    62461 accatgttca agatgccagt atttacctgg gcatcactgt gcgcgaacgt actgattatt
    62521 gcttccttcc caattctgac ggttaccgtc gcgttgttga ccctggatcg ctatctgggc
    62581 acccatttct ttaccaacga tatgggtggc aacatgatga tgtacatcaa cctgatttgg
    62641 gcctggggcc acccggaagt ttacatcctg atcctgcctg ttttcggtgt gttctccgaa
    62701 attgcggcaa ccttctcgcg taaacgtctg tttggttata cctcgctggt atgggcaacc
    62761 gtctgtatca ccgtgctgtc gttcatcgtt tggctgcacc acttctttac gatgggtgcg
    62821 ggcgcgaacg taaacgcctt ctttggtatc accacaatga ttatcgccat cccgaccggg
    62881 gtgaagatct tcaactggct gttcaccatg tatcagggcc gcatcgtgtt ccattctgcg
    62941 atgctgtgga ctatcggttt tatcgtcacc ttctcggtgg gcggtatgac tggcgtgctg
    63001 ctggcagtac cgggcgcgga cttcgttcta cataacagcc tgttcctgat tgcgcacttc
    63061 cataacgtga tcatcggcgg cgtggtcttc ggctgcttcg cagggatgac ctactggtgg
    63121 cctaaagcgt tcggtttcaa actgaacgaa acctggggta aacgcgcgtt ctggttctgg
    63181 atcatcggct tcttcgttgc ctttatgcca ctgtacgcgc tgggcttcat gggcatgacc
    63241 cgtcgtttga gccagcagat tgacccgcag ttccacacca tgctgatgat tgcagccagc
    63301 ggtgcagtac tgattgcgct aggtattctc tgcctcgtta ttcagatgta cgtttctatt
    63361 cgtgaccgcg accagaaccg tgacctgact ggcgacccgt ggggtggccg tacgctggag
    63421 tgggcaacct cttccccacc tccgttctat aactttgccg ttgtgccgca cgttcacgag
    63481 cgtgatgcat tctgggaaat gaaagagaaa ggcgaagcgt ataaaaagcc tgaccactat
    63541 gaagaaattc atatgccgaa aaacagcggt gcaggtatcg tcattgcagc tttctccacc
    63601 atcttcggtt tcgccatgat ctggcatatc tggtggctgg cgattgttgg cttcgcaggc
    63661 atgatcatca cctggatcgt gaaaagcttc gacgaggacg tggattacta cgtgccggtg
    63721 gcagaaatcg aaaaactgga aaaccagcat ttcgatgaga ttactaaggc agggctgaaa
    63781 aatggcaact gatactttga cgcacgcgac tgcccacgcg cacgaacacg ggcaccacga
    63841 tgcaggcgga accaaaatct tcggattttg gatctacctg atgagcgact gcattctgtt
    63901 ctctatcttg tttgctacct atgccgttct ggtgaacggc accgcaggcg gcccgacagg
    63961 taaggacatt ttcgaactgc cgttcgttct ggttgaaact ttcttgctgt tgttcagctc
    64021 catcacctac ggcatggcgg ctatcgccat gtacaaaaac aacaaaagcc aggttatctc
    64081 ctggctggcg ttgacctggt tgtttggcgc cggatttatc gggatggaaa tctacgaatt
    64141 ccatcacctg attgttaacg gcatggctcc ggatcgcagc ggcttcctgt cagcgttctt
    64201 tgcgctggtc ggcacgcacg gtctgcacgt cacttccggt cttatctgga tggcggtgct
    64261 gatggtacaa atcgctcgtc gcggcctgac cagcactaac cgtacccgca tcatgtgcct
    64321 gagcctgttc tggcacttcc tggatgtggt ttggatctgt gtgttcactg ttgtttatct
    64381 gatgggggcg atgtaatgag tcattctacc gatcacagcg gcgcgtccca tggcagcgta
    64441 aaaacctaca tgacaggctt tatcctgtcg atcattctga cggtgattcc gttctggatg
    64501 gtgatgacag gggctgcctc tccggccgta attctgggaa caatcctggc aatggcagtg
    64561 gtacaggttc tggtgcatct ggtgtgcttc ctgcacatga ataccaaatc agatgaaggc
    64621 tggaacatga cggcgtttgt cttcaccgtg ctaatcatcg ctatcctggt tgtaggctcc
    64681 atctggatta tgtggaacct caactacaac atgatgatgc actaagagcg gcggttatga
    64741 tgtttaagca atacctgcaa gtaacgaaac caggcatcat ctttggcaac ctgatctcgg
    64801 tgattggggg attcctgctg gcctcaaagg gcagcattga ttatcccctg tttatctaca
    64861 cgctggttgg ggtgtcactg gttgtggcgt cgggttgtgt gtttaacaac tacatcgaca
    64921 gggatatcga cagaaagatg gaaaggacga agaatcgggt gctggttaaa ggcctgatct
    64981 ctcctgctgt ctcgctggtg tacgccacct tgctgggtat tgctggcttt atgctgctgt
    65041 ggtttggcgc gaatccgctg gcctgctggc tgggggtgat gggctttgtg gtttatgtcg
    65101 gcgtttatag cctgtacatg aaacgccact cagtctacgg cacattgatt ggttcgctct
    65161 ccggcgctgc gccgccggtg attggttact gtgcagtaac cggcgagttc gatagcggcg
    65221 cagcgatcct gctggctatc ttcagcctgt ggcagatgcc tcactcctat gccatcgcca
    65281 ttttccgctt taaggattac caggcggcaa acattccggt attgccagtg gtaaaaggca
    65341 tttcggtggc gaagaatcac atcacgctgt atatcatcgc ctttgccgtt gccacgctga
    65401 tgctctctct tggcggttac gctggatata aatatctggt ggtcgccgcg gcggtgagcg
    65461 tctggtggtt aggtatggcc ctgcgcggtt ataaagttgc tgatgacaga atctgggcgc
    65521 gcaagctgtt cggcttctct atcatcgcca tcactgccct ctcggtgatg atgtccgttg
    65581 attttatggt gccggactcg catacgctgc tggctgctgt gtggtaacac aacctctcta
    65641 ttaaaaaggt gctacggcac cttttttctt agcattagaa acatatccct ctcgaaatat
    65701 ttactaaaaa atccgcatgt ttaccccatt cgtttgccgc tttacactag tcgcgaattt
    65761 aaaacagagg tggtaatgaa cgattataaa atgacgccag gtgagcggcg cgcgacctgg
    65821 ggtttaggga ccgtattctc gttgcgcatg ctgggcatgt tcatggttct gccggttctg
    65881 accacgtacg gcatggctct gcaaggtgcc agcgaagcat taatcggtat tgccattggt
    65941 atttatggtc tgactcaggc cgtttttcag attccgtttg gcctgctttc cgaccgcatt
    66001 ggtcgcaaac cattaattgt cggtgggctg gcagtgtttg ccgccggtag cgttatcgct
    66061 gcgctctctg actccatctg gggaattatt ctgggtcggg cgctacaagg ctccggtgcg
    66121 attgccgctg ccgttatggc gctgctttcc gatctcacgc gcgaacaaaa ccgcaccaaa
    66181 gcaatggcgt ttatcggcgt gagctttggc attacctttg ccattgcgat ggtgcttggc
    66241 ccgatcatca ctcacaaact tgggctgcac gcgctgttct ggatgatcgc tattctggca
    66301 acgaccggca ttgcgttgac catttgggtt gtgcccaaca gtagcactca cgtacttaat
    66361 cgtgagtccg gaatggtgaa aggcagtttc agtaaagtgc tggcggaacc gcggctgctg
    66421 aaactcaact ttggcattat gtgtctgcat attttgctga tgtcgacctt tgttgccctg
    66481 cccggacaac tggctgatgc ggggttcccg gcggctgaac actggaaggt ctatctggcg
    66541 acaatgctaa tcgcctttgg ctcggtcgtg cctttcatta tctacgctga agttaagcgc
    66601 aaaatgaagc aagtctttgt cttctgcatc gggttgatcg tggttgcgga aattgtgttg
    66661 tggaacgcgc aaacgcagtt ctggcaactg gtggtcggcg tgcagctttt ctttgtggcg
    66721 tttaatttga tggaagccct cctgccctca cttatcagta aagagtcgcc agcaggttac
    66781 aaaggtacgg cgatgggtgt ttactccacc agccagtttc ttggcgtggc gattggcggt
    66841 tcgctgggcg gctggattga cggcatgttt gacggtcagg gggtatttct cgctggcgca
    66901 atgctggccg cagtgtggct ggcagtcgcc agtaccatga aagaaccgcc gtatgtcagc
    66961 agtttgcgca ttgaaatccc ggcgaacatt gccgcaaacg aggcgttaaa agtgcgtttg
    67021 ctggaaactg aaggcatcaa agaagtgttg attgcagaag aagaacattc agcttatgtg
    67081 aaaatcgaca gcaaagtgac gaatcgcttt gaggttgaac aggcaattcg ccaggcataa
    67141 aagcgaggcg aaattgcctg ctgcactacg cttatcaggc ctactaagtt cctgagttac
    67201 aaagctttgt aggccgaatc aggcgttcat gccgcatctg acatgaacaa agcgcaggtt
    67261 gtcgcgatta atcgcggaag tttttgaact ggaacggctg acccagatcg ccaccgcgca
    67321 ccatcgccat gacagcctgc aaatcatcac gagatttgcc agtcacgcgg atctcatcgc
    67381 cctgaatttg cgcctgcact ttcagtttgc tgtctttgat catcttgacg attttcttct
    67441 gggtcgcgct ctcaatgccc tgtttcagtt tcgcttccac aaaccaggtt ttaccgctat
    67501 gaacgatatt ttccggtaca tccagcgaac tgccttcaat gccgcgcttc agcagcttgg
    67561 cacgcaaaat atccagcaac tgattgacct ggaaatcgga ctcgctcaac actttgatgg
    67621 ttttgctggc gtcgttcagc tcaaatgagg cttcaacgtt acggaagtca aaacgggact
    67681 ccacttcgcg gctcgcgtta tcgaccgcgt tacgtgcttc ctgaagatca acttcagaga
    67741 caatatcgaa agatggcatc ttttcctctc ccttcatttt tgatgcgaag cataatacct
    67801 gcaaagttaa ataaccaaca gtcctgcacc aggacgtatc ccttaattgt gcatagtcgc
    67861 aacagcatgc acaattaagg gatagcctta ttgaacaccc ggagtggttg cgggtgagga
    67921 ggaacaatga aaattaccgt attgggatgc ggtgccttag ggcaattatg gcttacagca
    67981 ctttgtaaac agggtcacga agttcagggc tggctgcgcg taccgcaacc ttattgtagt
    68041 gtgaatctgg ttgagactga tggttcgata tttaacgaat cgctgaccgc caacgatccc
    68101 gattttcttg ccaccagcga tctgctcctg gtgacgctga aagcatggca ggtttccgat
    68161 gccgtcaaaa gcctcgcgtc cacactgcct gtaactacgc caatactgtt aattcacaac
    68221 ggcatgggca ccatcgaaga attgcaaaac attcagcagc cattactgat gggcaccacc
    68281 acccatgccg cccgccgcga cggcaatgtc attattcatg tggcaaacgg tatcacgcat
    68341 attggtccag cacggcaaca ggacggcgat tacagttatc tggcggatat tttgcaaacc
    68401 gtgttgcctg acgttgcctg gcataacaat attcgcgccg agctgtggcg caagctggca
    68461 gtcaactgtg tgattaatcc actgactgcc atctggaatt gcccgaacgg tgaattacgt
    68521 catcatccgc aagaaattat gcaaatatgc gaagaagtcg cggcagtgat cgaacgcgaa
    68581 gggcatcata cttcagcgga agatttgcgt gattacgtga tgcaggtgat tgatgccaca
    68641 gcggaaaata tctcgtcgat gttgcaggat atccgcgcgc tgcgccacac cgaaatcgac
    68701 tatatcaatg gttttctctt acgccgcgcc cgcgcgcatg ggattgccgt accggaaaac
    68761 acccgcctgt ttgaaatggt aaaaagaaag gagagtgaat atgagcgcat cggcactggt
    68821 ttgcctcgcc cctggtagtg aagagactga agccgtcacc acgatcgacc tcttggttcg
    68881 cggcggtatc aaagtcacca ctgccagcgt agccagcgat ggcaacctgg cgattacctg
    68941 ctcgcgcggc gtgaagctgc tggcggatgc gccgctggtc gaagtggctg atggcgaata
    69001 tgacgtgatc gtactgcctg gtggcattaa aggcgcagag tgttttcgcg atagtaccct
    69061 gctggttgaa accgttaaac agttccaccg ttccgggcgt atcgtcgcgg ctatttgcgc
    69121 cgcgccagcc accgtgctgg tcccgcacga tatcttcccg attggcaata tgaccggctt
    69181 cccgacgctg aaagacaaaa ttcccgccga acaatggcag gacaagcgcg tcgtctggga
    69241 tgcacgggta aaattgctga ccagccaggg gccgggtaca gctatcgact ttggtctgaa
    69301 aattatcgac ctgttggttg ggcgtgaaaa agcccatgaa gtggcatcac aactggtgat
    69361 ggcggcaggg atttataatt attacgagta gtgtcggatg cggtaagcgt cgcatctgac
    69421 cagatgcgac gttacaacca caaattacgg gcgatatacc ttcacattgt taaagccctg
    69481 ctcgcgcaga tagagcgcct gcagacggct catcaccccg cgctcacacc acaacagcca
    69541 ggttttactc tggtcgagat cgccaaattt ggtgctcagt ttatagaacg gcagagaaac
    69601 cacatcaacc ccttcgactt tcagtggctt atcttcttgt tcatcgatag aacggatatc
    69661 gaggatgacg tcattcgggc cgaagccatt gacagtttcc acttccacca cttcctgctc
    69721 ggtctgctgg gcgatttcgc ggatatcaac gttattcgct tcctcaacca ctttatcgag
    69781 aatgctgaag tcgaacttct cttcttccgc ttcaatcttc gatttaaccg ctttcaccgt
    69841 cgggcttttg gagatcacac cgcagtattc cggcatcgtg cgagcaaagt cttcggtgcc
    69901 aatctggcga gccaggttga tgatgtgctc tttgtcgtaa gagatcagcg gacgcaggat
    69961 cagcgtatca gagacgttat caatcaggcg caggttggtc agcgtctgac tggacacctg
    70021 gccgagcgct tcgccggtga ccagcgcctg tacgccgtaa cgttcagcca ctttagacgc
    70081 ggcacgcacc atcatacgtt tgaggataac gcccatctga ccgtcgtcga ttttctcgag
    70141 aatttccccg acgaccggct cgaaattaat agcgacaaaa cgcacgcggt gggagctgcc
    70201 aaagcggttc cacagataat gcgccacctg acgtacgcca atttcatgcg ccgcgccgcc
    70261 gaggttaaag aagcagtaat gcacgcggca gccgcgacgc atcaacatat aactggaaac
    70321 accggagtcg aaaccaccgg aaatgagcga cagtacatct tcctgggtgc cgatcgggaa
    70381 accaccaata ccttcgtagc ggcctttaat cagcaggaga cgatcgtctt ccacttccaa
    70441 atggacagtc acatccggat tggtcagctt cacgcgcgcg gattcaatat gctgatttaa
    70501 accgccgccg acgtaacgtt ccacatcaat cgagctaaaa tcatgtttgc cacggcgctt
    70561 cacgcgtacg cagaaggttt tgccttccag ctgatcgcga tactgaacca acgctttctc
    70621 gaaaatatcg tgcatgtcgg taaacggcac atcttcgact tcgagaatat ggtggatacc
    70681 cggaatacgg gtcagagcgt cgcgaatagc cagacgctgg ttttcatctt ttgcgcgaac
    70741 ttcgatgtta tcccagtggc ggacgacagc gagcgtctca tcatagtgct ttaaaacgtt
    70801 acgaatgttc ccggtaagga tttttataaa gcgcaagcgc acagattggc ttttgatggt
    70861 gatttccggg aacaatttaa tgataaactt catggcggca atggttcgtt ggcaagcctt
    70921 aagcgacttg tatagggaaa aatacagcag cccacacctg cggctgcatc caggcgcgga
    70981 agtataccac taacatcgct ttgctgcgca catcacctta ccattgcgcg ttatttgcta
    71041 tttgccctga gtccgttacc atgacggggc gaaaaatatt gagagtcaga cattcattat
    71101 gccgaagaaa aatgaggcgc ccgccagctt tgaaaaggcg ctgagcgagc tggaacagat
    71161 tgtaacccgt ctggaaagtg gcgacctgcc gctggaagag gcgctgaacg agttcgaacg
    71221 cggcgtgcag ctggcacgtc aggggcaggc caaattacaa caagccgaac agcgcgtaca
    71281 aattctgctg tctgacaatg aagacgcctc tctaacccct tttacaccgg acaatgagta
    71341 atggactttc cgcagcaact cgaagcctgc gttaagcagg ccaaccaggc gctgagccgt
    71401 tttatcgccc cactgccctt tcagaacact cccgtggtcg aaaccatgca gtatggcgca
    71461 ttattaggtg gtaagcgcct gcgacctttt ctggtttatg ccaccggtca tatgttcggc
    71521 gttagcacaa acacgctgga cgcacccgct gctgccgttg agtgtatcca cgcttactca
    71581 ttaattcatg atgatttacc ggcgatggat gatgacgatc tgcgtcgcgg tttgccgacc
    71641 tgccatgtga agtttggcga agcgaacgcg attctcgctg gcgatgcttt acaaacgctg
    71701 gcgttctcga ttctaagcga tgctgatatg ccggaagtgt cggatcgcga cagaatttcg
    71761 atgatttctg aactggcgag cgccagcggt attgccggaa tgtgcggtgg tcaggcatta
    71821 gatttagacg cggaaggcaa acatgtacct ctggacgcac tcgagcgtat tcatcgtcat
    71881 aaaaccggcg cattgattcg cgccgccgtt cgccttggtg cattaagcgc cggagataaa
    71941 gggcgtcgtg ctctgccagt actcgacaag tacgcagaga gcatcggcct tgccttccag
    72001 gttcaggatg acatcctgga tgtggtggga gatactgcaa cgttgggaaa acgccagggt
    72061 gccgaccagc aacttggtaa aagtacctac cctgcacttc tgggtcttga gcaagcccgg
    72121 aagaaagccc gggatctgat cgacgatgcc cgccagtcgc tgaaacaact ggctgaacag
    72181 tcactcgata cctcggcact ggaagcgcta gcggactaca tcatccagcg taataaataa
    72241 acaataagta ttaataggcc cctgatgagt tttgatattg ccaaataccc gaccctggca
    72301 ctggtcgact ccacccagga gttacgactg ttgccaaaag agagtttacc gaaactctgc
    72361 gacgaactgc gccgctattt actcgacagc gtgagccgtt ccagtgggca cttcgcctcc
    72421 gggctgggca cggtcgagct gaccgtggcg ctgcactacg tctataacac tccgtttgac
    72481 cagttaatct gggatgtggg gcatcaggct tatccgcata aaattttgac cggacgccgc
    72541 gacaaaatcg gcaccatccg tcagaaaggt ggcctgcacc cgttcccgtg gcgcggcgaa
    72601 agcgaatatg acgtattaag cgtcggacat tcatcaacct ccatcagtgc cggaattggt
    72661 attgcggttg ctgccgagaa agaaggcaaa aatcgccgca ccgtctgtgt gattggcgat
    72721 ggcgcgatta ccgcaggcat ggcgtttgaa gcgatgaatc acgcgggcga tatccgtcct
    72781 gatatgctgg tggttctcaa cgacaatgaa atgtcgattt ccgaaaatgt cggtgcgctc
    72841 aacaaccatc tggcacagct gctttccggt aagctttact cttcactgcg cgaaggcggg
    72901 aaaaaagttt tctctggcgt tccgcccatt aaagagctgc tcaaacgtac cgaagaacat
    72961 attaaaggca tggtggtgcc tggcacattg tttgaagagc tgggctttaa ctacatcggc
    73021 ccggtggacg gtcacgatgt gctggggctt atcaccacgc tgaagaacat gcgcgacctg
    73081 aaaggcccgc agtttctgca tatcatgacc aaaaaaggtc gtggttatga accggcagaa
    73141 aaagacccaa tcaccttcca cgctgtgcct aaatttgatc cctccagcgg ttgtttgccg
    73201 aaaagtagcg gtggtttgcc gagctattca aaaatctttg gcgactggtt gtgcgaaacg
    73261 gcagcgaaag ataacaagct gatggcgatt actccggcga tgcgtgaagg ttccggcatg
    73321 gtcgagtttt cacgtaaatt cccggatcgc tacttcgatg tggcaattgc cgagcaacac
    73381 gcggtgacct ttgccgccgg tctggcgatt ggtgggtaca aacccattgt cgcgatttac
    73441 tccactttcc tgcaacgcgc ctatgatcag gtgttgcatg acgtggcgat tcaaaagctt
    73501 ccggtcctgt tcgccatcga ccgtgcgggc attgttggtg ctgacggtca aacccatcag
    73561 ggcgcttttg atctctctta tctgcgctgc ataccggaaa tggtcattat gactccgagc
    73621 gatgaaaacg aatgtcgcct gatgctctat accggctatc actataacga tggcccgtca
    73681 gcggtgcgct acccgcgtgg caacgcggtc ggcgtggaac tgacgccgct ggaaaaacta
    73741 ccaattggca aaggcattgt gaagcgtcgt ggcgagaaac tggcgatcct taactttggt
    73801 acgctgatgc cagaagcggc gaaagtcgcc gaatcgctga acgccacgct ggtcgatatg
    73861 cgttttgtga aaccgcttga tgaaacgtta attctggaaa tggccgccag tcatgaagca
    73921 ctggttaccg tagaagaaaa cgccattatg ggcggtgcag gcagcggagt gaacgaagtg
    73981 ctgatggccc atcgtaaacc agtacccgtg ctgaacattg gcctgccgga cttctttatt
    74041 ccgcaaggaa ctcaggaaga aatgcgcgcc gaactcggcc tcgatgccgc cggtatggaa
    74101 gccaaaatca aggcctggct ggcataatcc ctactccact cctgctatgc ttaagaaatt
    74161 attcatggca ggagtggcag catgcaatac aaccccttag gaaaaaccga ccttcgcgtt
    74221 tcccgacttt gcctcggctg tatgaccttt ggcgagccag atcgtggtaa tcacgcatgg
    74281 acactgccgg aagagagcag ccgtcccata attaaacgcg cgctggaagg cggcataaat
    74341 ttctttgata ccgccaatag ctattccgat ggcagcagcg aagagatcgt cggtcgcgca
    74401 ctgcgggatt tctcccgtcg tgaagacgtg gtcgttgcca ccaaagtgtt ccatcgcgtt
    74461 ggtgatttac cggaaggatt atcccgtgca caaattttgc gctctatcga cgacagcctg
    74521 cgacgtctcg gcatggatta tgtcgatatc ctgcaaattc atcgctggga ttacaacacg
    74581 ccgatcgaag agacgctgga agccctcaac gacgtggtaa aagccgggaa agcgcgttat
    74641 atcggcgcgt catcaatgca cgcttcgcag tttgctcagg cgctggagct acaaaaacag
    74701 cacggctggg cgcagtttgt cagtatgcag gatcactaca atctgattta tcgcgaagaa
    74761 gagcgcgaga tgctgccgct gtgttatcag gaaggcgtgg cggtgattcc gtggagcccg
    74821 ctggcacggg gacgtctgac gcgtccgtgg ggagaaacca ctgcacgact ggtgtctgat
    74881 gaggtgggga aaaatctcta taaagaaagc gatgagaatg acgcgcaaat tgcggagcgg
    74941 ttaacgggcg tcagtgaaga tctgggcgcg acacgagcac aagtcgcact ggcatggttg
    75001 ttgagcaaac ctggcattgc tgcaccgatt atcggtacat cgcgggaaga acagcttgat
    75061 gagctactga acgcggtgga tatcactttg aagccagaac agattgccga actggaaacg
    75121 ccgtataaac cgcatccggt cgtaggattt aagtaaattt cgcttttcgc ccattttatc
    75181 tgctgaattc cccatcgtct gcgatgatgg ggacaacgta cctcccatca ccttctgtaa
    75241 ccataatcca cctctgcaag agaaattctt cgtgattaaa tttacagcta ttcatcatct
    75301 tcatcgcttt gagcgatttt tgatagtttc tcccgataat atcccctaaa aatgtcttat
    75361 ttagtcaatg taagaggatt acaatcatga tgaaaaccat caccaaacaa ccgatactgt
    75421 ttactgatgt accggttgca gatttaagaa actcaatgaa gcaagattta aatcaaaatc
    75481 ttattgaaag gctgtggaac aaaattcgtg atttttttct ggacagtgat aaacaaaagg
    75541 catttaaatc gattcataaa tacatcaata cattgtcagt tctaaattat aattcagcgt
    75601 taactccaga tcccaacttt aatatagatg caacgtcaga tttagactca tatttaaagc
    75661 tggattttga tcgcctctcg cccaaacaaa agcagaccac gctttgttgt ttctggaata
    75721 aaattgcatc ctcattgcca gagccataca acagtaccat caaacataac attatttttt
    75781 ataaagacgg agaaaatctg atgatacgtg gaactatctc tattgttaac gaggtagtaa
    75841 agacttattc actcccaata gaaaaggatg acaatggata ttacgatttc agcggtctat
    75901 atctagcaca cagcaatatt tccggtaaag atcccaataa agatcccgat atagatttcg
    75961 gtatagatat gggaaactgt aactgctcaa atgtcaactt cgaacatact tatttttatg
    76021 gcgtcaagtt tacaaatgcg aattgtacaa atgcgaattt taacaattgt agatttaaga
    76081 aatgcgacct gacaaatatg aattgcactg gcgcaattct tgataatgcg atgatatatg
    76141 gtaaagaaaa agaacctgag atgcaatatc cagaagcaga tcaaataata caaaggataa
    76201 cttatcagaa atcagatggg aatgaaacga aagggatgat tctaactaac tgctcctgtg
    76261 ttaaaaccac tttcaattgg gcagatttat cagaaagtga ttgccaaaat gtggacttta
    76321 gcgaggctaa tctttcaaac actattcttc ctgacatcgt caggatgaaa ggcacgaaac
    76381 tttaccgtac cgaccttttt aatccgatat taaaaacaga agcagaatca acagaagaaa
    76441 aagacattag cccattagca aaaattattc ttgactat
//
LOCUS       DAZFKF010000012        76394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFKF010000012 DAZFKF010000000
VERSION     DAZFKF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.012"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(79..876)
                     /gene="rfaP"
                     /locus_tag="ACHKA1_000979"
     CDS             complement(79..876)
                     /gene="rfaP"
                     /locus_tag="ACHKA1_000979"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO5678674.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPSDVRVKRMLIKRVATMVRNMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(869..1993)
                     /gene="waaG"
                     /locus_tag="ACHKA1_000980"
     CDS             complement(869..1993)
                     /gene="waaG"
                     /locus_tag="ACHKA1_000980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO5678675.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVEANCGEAIVEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(1990..3048)
                     /gene="rfaQ"
                     /locus_tag="ACHKA1_000981"
     CDS             complement(1990..3048)
                     /gene="rfaQ"
                     /locus_tag="ACHKA1_000981"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO5678676.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTSLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            3461..4738
                     /gene="waaA"
                     /locus_tag="ACHKA1_000982"
     CDS             3461..4738
                     /gene="waaA"
                     /locus_tag="ACHKA1_000982"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO5678677.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSSSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4746..5225
                     /gene="coaD"
                     /locus_tag="ACHKA1_000983"
     CDS             4746..5225
                     /gene="coaD"
                     /locus_tag="ACHKA1_000983"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO5678678.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(5264..6073)
                     /gene="mutM"
                     /locus_tag="ACHKA1_000984"
     CDS             complement(5264..6073)
                     /gene="mutM"
                     /locus_tag="ACHKA1_000984"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO5678679.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(6171..6338)
                     /gene="rpmG"
                     /locus_tag="ACHKA1_000985"
     CDS             complement(6171..6338)
                     /gene="rpmG"
                     /locus_tag="ACHKA1_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO5678680.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(6359..6595)
                     /gene="rpmB"
                     /locus_tag="ACHKA1_000986"
     CDS             complement(6359..6595)
                     /gene="rpmB"
                     /locus_tag="ACHKA1_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO5678681.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(6812..7480)
                     /gene="radC"
                     /locus_tag="ACHKA1_000987"
     CDS             complement(6812..7480)
                     /gene="radC"
                     /locus_tag="ACHKA1_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO5678682.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            7652..8872
                     /gene="coaBC"
                     /locus_tag="ACHKA1_000988"
     CDS             7652..8872
                     /gene="coaBC"
                     /locus_tag="ACHKA1_000988"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO5678683.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            8850..9308
                     /gene="dut"
                     /locus_tag="ACHKA1_000989"
     CDS             8850..9308
                     /gene="dut"
                     /locus_tag="ACHKA1_000989"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO5678684.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            9415..10011
                     /gene="slmA"
                     /locus_tag="ACHKA1_000990"
     CDS             9415..10011
                     /gene="slmA"
                     /locus_tag="ACHKA1_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO5678685.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(10048..10689)
                     /gene="pyrE"
                     /locus_tag="ACHKA1_000991"
     CDS             complement(10048..10689)
                     /gene="pyrE"
                     /locus_tag="ACHKA1_000991"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO5678686.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(10755..11471)
                     /gene="rph"
                     /locus_tag="ACHKA1_000992"
     CDS             complement(10755..11471)
                     /gene="rph"
                     /locus_tag="ACHKA1_000992"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO5678687.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            11598..12461
                     /gene="yicC"
                     /locus_tag="ACHKA1_000993"
     CDS             11598..12461
                     /gene="yicC"
                     /locus_tag="ACHKA1_000993"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO5678688.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKALIEQRL
                     EGVTAEVVKVRSHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNVE"
     gene            12652..13914
                     /locus_tag="ACHKA1_000994"
     CDS             12652..13914
                     /locus_tag="ACHKA1_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5678689.1"
                     /translation="MPKLTDIQIRAWIKADERFDGKADGNGLYLCYPKNYTMPFWRFR
                     YKFAGKQRAMVIGSYAELSLSRARETAKELSARVALGYDVAAEKQERKAEALAKMEAE
                     KNAIRVSELAAEYFERQILPRWKHPDILRRRIDKDINPRIGHMKAEDVKPRHIDEMLK
                     AIVDRGAPTIATDVLRWTRRIFDYGIKRHVLEINPCSAFEVSDAGGKESSRDRWLTRE
                     ELIRFFQAMRTAKGFSRQNELTFKLLLALCVRKMELCAARWEEFDLDNAVWHLPEERS
                     KNGDPIDIPLVPPVIEWLKELRVLSCNSAWVLPARKMQHRMIPHIQESTLPVALAKVR
                     AEMPEVPDFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRYQYFDERTS
                     ALAKWTNLLVSLERGERYNVTPIRRAKA"
     gene            13911..14561
                     /locus_tag="ACHKA1_000995"
     CDS             13911..14561
                     /locus_tag="ACHKA1_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678690.1"
                     /translation="MINILKKSPIERLCASVSITPHEMALALAGLNPSMRIGDVPQDK
                     FEQVESARTMIARAIWLHSGKKAGKDEPYRAGDIFLASFPFIEAGTPEAIITAVTDAI
                     DDLRGTKNWEEKALNLGGRRLVSHIKETSRSGRGQYRKLDEEQGNMKMMGLLVLLLVK
                     KSGTTAYIQDGEPNRSAIYRDVEALMKEKGISPKGIAKSTFMQKISAALLAVSQAD"
     gene            14948..15163
                     /locus_tag="ACHKA1_000996"
     CDS             14948..15163
                     /locus_tag="ACHKA1_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5678691.1"
                     /translation="MTANHQEAIPTTGYIRRCNLAKLLDTSVSTIDRWVRIGKLPKPT
                     KLGEKNTVFNAVEINHWLNERRNNTKQ"
     gene            15231..15950
                     /locus_tag="ACHKA1_000997"
     CDS             15231..15950
                     /locus_tag="ACHKA1_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5678692.1"
                     /translation="MKRPNMPVQGQGFVQPEISQNDILIRHKAESRIDSRVISERAGI
                     QHESIIATIKSHQSRLRELGSLPRQSVKELTYLKSGKSGRKRGRPEISYLLNELQLDY
                     LLRVIRGRDAERINQFKLDVTKAFAKHRAAEPVRREYLPGYHESRDGLKALGAERHHY
                     INLARAENRVTGLVAGERSTASEQQLGLLIVMQKIEQAAFDEAIQSDMTPTEAVREVA
                     RRMETFASLMTSASSLGVKHA"
     gene            15943..16419
                     /locus_tag="ACHKA1_000998"
                     /pseudo
     CDS             15943..16419
                     /locus_tag="ACHKA1_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16512..16691)
                     /locus_tag="ACHKA1_000999"
     CDS             complement(16512..16691)
                     /locus_tag="ACHKA1_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5678693.1"
                     /translation="MATKAVNAKSQTVAARVPHEVMNNVEAVKMPGESTGQFVTAALK
                     REVEYRQRRKAKESE"
     gene            16884..17081
                     /locus_tag="ACHKA1_001000"
     CDS             16884..17081
                     /locus_tag="ACHKA1_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5678694.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQEASHG"
     gene            17086..17538
                     /locus_tag="ACHKA1_001001"
                     /pseudo
     CDS             17086..17538
                     /locus_tag="ACHKA1_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            17531..17716
                     /locus_tag="ACHKA1_001002"
     CDS             17531..17716
                     /locus_tag="ACHKA1_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678695.1"
                     /translation="MLKTFRVFSRAVNKRGYTVGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDEE"
     gene            17720..17929
                     /locus_tag="ACHKA1_001003"
     CDS             17720..17929
                     /locus_tag="ACHKA1_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678696.1"
                     /translation="MCDFSDMTVDEAAKSAMQAELICSLMMGNTGGMTEGEIESLLAL
                     IKQLTGKAGSWLIAASGDDAICVND"
     gene            17974..18177
                     /locus_tag="ACHKA1_001004"
     CDS             17974..18177
                     /locus_tag="ACHKA1_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678697.1"
                     /translation="MQQITLAEASLQAQQVEVISRMIERMIERNHEEIDDHDLSVIAG
                     LIKQLSSNVAVWLMTEEEKRGEQ"
     gene            18235..18357
                     /locus_tag="ACHKA1_001005"
     CDS             18235..18357
                     /locus_tag="ACHKA1_001005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678698.1"
                     /translation="MASKNTGLTASSKTHPEFMCGDIYRNEYGSHMTMISRGKK"
     gene            18354..20492
                     /locus_tag="ACHKA1_001006"
     CDS             18354..20492
                     /locus_tag="ACHKA1_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFO5678699.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWQEQDGKLA
                     GDKTPPVVLADEQLKDIGNLQIVPDGRKSARIYKAGHLDQVMVKGIGQKLAAAGVQDA
                     DYYPEGMHHNERQNWRNYLETERKNISDGLVIELPVKKKTSEQSEDELKPRVECRPDG
                     VYWVTPKVDKQSGEIIRPCEWIGDRMRTVGIGHDGCDGYLIIELEPEGMEKIIYEAMP
                     RNEIGLPSGWSRLRGCGVAVTTSQKLLNKLAEYLQREGERTQWEVTSTAGWHCGTYVM
                     PDGEIIGVPERPVAFCGGSAAIKGYVVRGTADEWRDNVASLMRGNHSMMLGVLVGLAA
                     PLNSLVGGGCFGVHLFAQSSAGKTTTVEAATSLYGDPEVLKLSWDATRYGLTVEAAAR
                     NDGFIPIDEIGQGGRINDIAQSAYSLFNGVGRIQGRKDGGNRAVIRWRIAALSTGEED
                     FETFLIKGGVTPKAGQLVRLLSVPFVDTTVFNGYDDGDQHARAIKRFASNYCGAAGRE
                     WIGWLSANKELAVSVVNEKENIWLSRLPENASPQVKRVASRFAMLDAAGELATGITGW
                     TAGESHVATQRAFDDWLQDFGLDNREKYQVISRARDFIQRHALSRFQPYTYGRQNGDM
                     DVNYGARITSLAGYLVRGRRDDGLPEYHIIPSVFDEEILCGINRNFGCQALKEAGILI
                     HAGDKNWTTKTIKVNGIQQRFIVLLDQPEE"
     gene            20907..21680
                     /locus_tag="ACHKA1_001007"
     CDS             20907..21680
                     /locus_tag="ACHKA1_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678700.1"
                     /translation="MCKEKNFYQCMSSREREDLKSFVTRCEIRGDIQSLSRTLIMIAH
                     WMRQGNPVNFTEYASQWTEAQLERDDGNHSTPEMAQQWPFSGKRCIRKGYSDYYPFGV
                     DGSRQDDETEIKHAVTVILASWPLFNRDGLDLYNHNKAWEHPLDYVCFMEEAKSCLRW
                     IRENSLTNDKIASFPEKNPTSYGLKHCVERANKRLTLENGMPTEPTYISNGAFIAAMV
                     ASGYKIKPAGRMNCRFNISHKQLKRVLSGELYKAGEWTI"
     gene            21677..22126
                     /locus_tag="ACHKA1_001008"
     CDS             21677..22126
                     /locus_tag="ACHKA1_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5678701.1"
                     /translation="MTAQIAAYGRLVDDPQEKQTSKGTAMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGETRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGQQGNQPPAGGDDPFSDDIPF"
     gene            22143..22559
                     /locus_tag="ACHKA1_001009"
     CDS             22143..22559
                     /locus_tag="ACHKA1_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO5678702.1"
                     /translation="MNQQTTQQDQTTRRPTERPSWRHMTKRQRKNRRRINRLAEYWPE
                     LFGAETIKPLKTGIIRDVLEDAKSRELPIGHGVLCGALTSYIHTTRYLKAIVAGGPRY
                     GLNGQPCGEVTSDEKAVARGILKQRLEQMKQKNIQQ"
     gene            22579..22872
                     /locus_tag="ACHKA1_001010"
     CDS             22579..22872
                     /locus_tag="ACHKA1_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5678703.1"
                     /translation="MITDSVAQKLEERGLWRRAATRWAEVLLHAETDREREEAARRRA
                     ICIIKSRRMPEQLVTFGDVKKAADRTLKEMGINPQDEWRSYSFSDAGDDLTLP"
     gene            complement(23319..23705)
                     /locus_tag="ACHKA1_001011"
     CDS             complement(23319..23705)
                     /locus_tag="ACHKA1_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678704.1"
                     /translation="MRLTQYKKEILSYFTPDNLAWVTAEIGSPPFDVSGVAYLLYGMD
                     SHNNRYQLESTRRTLEAMVRDGLLERVNVYERRDSVGHGVRRKVARYGLPGRSVVARD
                     GEGRNGAIDGECIRINATGVQLVDGQ"
     gene            23790..24092
                     /locus_tag="ACHKA1_001012"
     CDS             23790..24092
                     /locus_tag="ACHKA1_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5678705.1"
                     /translation="MIRDDKAEALENSGLWRRAAERWGELFRQAKTDEERHYITQRRA
                     QCTAKATRPKTTEWGHVCAVRAAATRTLKDMGIDQKKEDPLRGTKYRGETGRRDGS"
     gene            24094..24261
                     /locus_tag="ACHKA1_001013"
     CDS             24094..24261
                     /locus_tag="ACHKA1_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5678706.1"
                     /translation="MDTITDKKAEQLESQGLWRRAAARWLDVMKEAHTDPQREHIARR
                     REICLANFRML"
     gene            24491..24730
                     /locus_tag="ACHKA1_001014"
     CDS             24491..24730
                     /locus_tag="ACHKA1_001014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678707.1"
                     /translation="MRKKLRKKVRKSIIKPITKSITQTNRAKPDMTRLAGGKATGKSA
                     QKNAQNETQQPRKTKPIRDDRKSAQIYRIRVSNFA"
     gene            24799..24960
                     /locus_tag="ACHKA1_001015"
     CDS             24799..24960
                     /locus_tag="ACHKA1_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678708.1"
                     /translation="MTERSPTEIIHGIIEEQEERELILMIEEQRERQRKQEATQPDSV
                     SAENQADVT"
     gene            25398..25790
                     /locus_tag="ACHKA1_001016"
     CDS             25398..25790
                     /locus_tag="ACHKA1_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5678709.1"
                     /translation="MRDIQQVLERWGAWAANNRENVTWSSVAAGFKGIIPAKITSRSQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGKTFMALAHKHECSDSLIGKRLQKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
     gene            26080..26286
                     /locus_tag="ACHKA1_001017"
     CDS             26080..26286
                     /locus_tag="ACHKA1_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678710.1"
                     /translation="MEGVVSHEQAGHRASKNHRFVLFFSQGKNATGINPSSGGISRQH
                     DEQAAQPDTASRRYYDFRNTFSKA"
     gene            complement(27001..27375)
                     /locus_tag="ACHKA1_001018"
     CDS             complement(27001..27375)
                     /locus_tag="ACHKA1_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678711.1"
                     /translation="MDTHDKIPPAYSYSPQPVPEAWKMRFSANSAWFFTDDFSVVNAW
                     LKAHSDGVEVVPLFCGSDSDGKRDTAFEEVCAALARMLHMHRLLLEKTRYQEHVTDPE
                     CLRELHEAPIQALNILVKHKLS"
     gene            28153..28977
                     /gene="dinD"
                     /locus_tag="ACHKA1_001019"
     CDS             28153..28977
                     /gene="dinD"
                     /locus_tag="ACHKA1_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO5678712.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQISIKQLENCVKITEKK"
     gene            29269..29886
                     /gene="yicG"
                     /locus_tag="ACHKA1_001020"
     CDS             29269..29886
                     /gene="yicG"
                     /locus_tag="ACHKA1_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO5678713.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(29883..31565)
                     /gene="ligB"
                     /locus_tag="ACHKA1_001021"
     CDS             complement(29883..31565)
                     /gene="ligB"
                     /locus_tag="ACHKA1_001021"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO5678714.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVADVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNQARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLVSTEQFWQQLPGIGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            31823..32446
                     /gene="gmk"
                     /locus_tag="ACHKA1_001022"
     CDS             31823..32446
                     /gene="gmk"
                     /locus_tag="ACHKA1_001022"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO5678715.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            32501..32776
                     /gene="rpoZ"
                     /locus_tag="ACHKA1_001023"
     CDS             32501..32776
                     /gene="rpoZ"
                     /locus_tag="ACHKA1_001023"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO5678716.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            32795..34903
                     /gene="spoT"
                     /locus_tag="ACHKA1_001024"
     CDS             32795..34903
                     /gene="spoT"
                     /locus_tag="ACHKA1_001024"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO5678717.1"
                     /translation="MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            34910..35599
                     /gene="trmH"
                     /locus_tag="ACHKA1_001025"
     CDS             34910..35599
                     /gene="trmH"
                     /locus_tag="ACHKA1_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO5678718.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITEEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            35605..37686
                     /gene="recG"
                     /locus_tag="ACHKA1_001026"
     CDS             35605..37686
                     /gene="recG"
                     /locus_tag="ACHKA1_001026"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO5678719.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLQTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALNVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(37862..39067)
                     /gene="gltS"
                     /locus_tag="ACHKA1_001027"
     CDS             complement(37862..39067)
                     /gene="gltS"
                     /locus_tag="ACHKA1_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO5678720.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            39346..40737
                     /gene="xanP"
                     /locus_tag="ACHKA1_001028"
     CDS             39346..40737
                     /gene="xanP"
                     /locus_tag="ACHKA1_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO5678721.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            40858..42567
                     /gene="yicH"
                     /locus_tag="ACHKA1_001029"
     CDS             40858..42567
                     /gene="yicH"
                     /locus_tag="ACHKA1_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO5678722.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLLLDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PAQ"
     gene            complement(42611..43291)
                     /locus_tag="ACHKA1_001030"
     CDS             complement(42611..43291)
                     /locus_tag="ACHKA1_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO5678723.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEASLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CSEYGILR"
     gene            complement(43272..44204)
                     /locus_tag="ACHKA1_001031"
     CDS             complement(43272..44204)
                     /locus_tag="ACHKA1_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO5678724.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            complement(44252..45112)
                     /locus_tag="ACHKA1_001032"
     CDS             complement(44252..45112)
                     /locus_tag="ACHKA1_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO5678725.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMNILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            complement(45193..46044)
                     /locus_tag="ACHKA1_001033"
     CDS             complement(45193..46044)
                     /locus_tag="ACHKA1_001033"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO5678726.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAYAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVTLALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADMMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEIFHCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGSIGKA"
     gene            complement(46056..47147)
                     /locus_tag="ACHKA1_001034"
     CDS             complement(46056..47147)
                     /locus_tag="ACHKA1_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO5678727.1"
                     /translation="MKDNKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            complement(47172..47486)
                     /locus_tag="ACHKA1_001035"
     CDS             complement(47172..47486)
                     /locus_tag="ACHKA1_001035"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO5678728.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            complement(47504..47974)
                     /locus_tag="ACHKA1_001036"
     CDS             complement(47504..47974)
                     /locus_tag="ACHKA1_001036"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFO5678729.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            complement(48001..49554)
                     /locus_tag="ACHKA1_001037"
     CDS             complement(48001..49554)
                     /locus_tag="ACHKA1_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFO5678730.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVKIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLGAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            complement(49849..52167)
                     /gene="yicI"
                     /locus_tag="ACHKA1_001038"
     CDS             complement(49849..52167)
                     /gene="yicI"
                     /locus_tag="ACHKA1_001038"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO5678731.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKETVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANAQGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYAW
                     HEGTAFQLFNLQDGHEAVCEVPAADGSVIFTLKATRTGNTITVTGAGEVRNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(52177..53559)
                     /locus_tag="ACHKA1_001039"
     CDS             complement(52177..53559)
                     /locus_tag="ACHKA1_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO5678732.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISIAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIVAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            53852..53946
                     /locus_tag="ACHKA1_001040"
     tRNA            53852..53946
                     /locus_tag="ACHKA1_001040"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53886..53888,aa:Sec,seq:tca)
     gene            54246..55430
                     /locus_tag="ACHKA1_001041"
     CDS             54246..55430
                     /locus_tag="ACHKA1_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5678733.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQISLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            55842..56885
                     /locus_tag="ACHKA1_001042"
     CDS             55842..56885
                     /locus_tag="ACHKA1_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO5678734.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAVGMKHSSIQHQ"
     gene            57400..60915
                     /locus_tag="ACHKA1_001043"
     CDS             57400..60915
                     /locus_tag="ACHKA1_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO5678735.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASF
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLNNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMARIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLEEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREKSNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAAWLAENQQNIEAHYGKSLHEVIGIVTPFSAQVSTIKQALGKQGISTGANEKSL
                     TVGTVHSLQGAERAIVVFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPPSSPRGLLAKYLFESEKNALSFDYKERKDLKTSGTKIYTLHGVEQHDKFLNQTF
                     ENTSKYITIVSPWLTWQKLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHKDYEKRKE
                     KQQNLKGTLEKLNAFGITTKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLEKRQV"
     gene            complement(61024..62523)
                     /locus_tag="ACHKA1_001044"
     CDS             complement(61024..62523)
                     /locus_tag="ACHKA1_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO5678736.1"
                     /translation="MNIRKLFCPGNTPRILLFLFFFVVSAITSIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFIVIFWRAHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     ATDTLREMQRYKQLSQHGADNWKILPGVPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSTAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMIGSHPNPCDRLNSWPNHYLEQYPRKIACYLASISKLDNFLGQFDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLINTASDITSHQSVSRKISARHFTGIFQ
                     WLTGIRTENIPPFNPLTDEDNEPVMVFNGEKNVPADSLKPQPLILPDRR"
     gene            complement(62705..63445)
                     /locus_tag="ACHKA1_001045"
     CDS             complement(62705..63445)
                     /locus_tag="ACHKA1_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFO5678737.1"
                     /translation="MKKVIVVSVFAMAGMFSAQALADESKTGFYMTGKAGTSVLSLSN
                     QRLIDGEGEWADKYKGGNDHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NVDKDSWSGGYWRDDLKNEVSVNTLMLNTYYDFRNDSAFTPWISAGIGYARVHQKTTG
                     ISIWDHGYGNSGRESLSRSGSADNFAWSFGAGVRYDVTPDIALDLSYRYLDAGDASVS
                     YKDEWGDKYKSEVDVKSHDIMLGMTYNF"
     gene            63871..65697
                     /gene="atoS"
                     /locus_tag="ACHKA1_001046"
     CDS             63871..65697
                     /gene="atoS"
                     /locus_tag="ACHKA1_001046"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFO5678738.1"
                     /translation="MNYIKWIYPRRLRNQMILMAILMVIVPTLAIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALHETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRQELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDLIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARIDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            65694..67079
                     /gene="atoC"
                     /locus_tag="ACHKA1_001047"
     CDS             65694..67079
                     /gene="atoC"
                     /locus_tag="ACHKA1_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFO5678739.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRPPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTSPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            67275..67937
                     /gene="atoD"
                     /locus_tag="ACHKA1_001048"
     CDS             67275..67937
                     /gene="atoD"
                     /locus_tag="ACHKA1_001048"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFO5678740.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            67937..68587
                     /gene="atoA"
                     /locus_tag="ACHKA1_001049"
     CDS             67937..68587
                     /gene="atoA"
                     /locus_tag="ACHKA1_001049"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFO5678741.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGARKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            68584..69906
                     /gene="atoE"
                     /locus_tag="ACHKA1_001050"
     CDS             68584..69906
                     /gene="atoE"
                     /locus_tag="ACHKA1_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFO5678742.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLQKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            69937..71121
                     /gene="atoB"
                     /locus_tag="ACHKA1_001051"
     CDS             69937..71121
                     /gene="atoB"
                     /locus_tag="ACHKA1_001051"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFO5678743.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMDESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(71531..71716)
                     /locus_tag="ACHKA1_001052"
                     /pseudo
     CDS             complement(71531..71716)
                     /locus_tag="ACHKA1_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063945310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            71777..72910
                     /locus_tag="ACHKA1_001053"
     CDS             71777..72910
                     /locus_tag="ACHKA1_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc45 family transposase"
                     /protein_id="HFO5678744.1"
                     /translation="MHSENIAAYIGLDVHKETLAVAIAAPERLGEVRYYGTINNEAQA
                     VRRLFQKLQGLYGNILSCYEAGPCGFGLYHQLTAMNIKCQVIAPSRIPKSPTDRIKND
                     HRDAISLARLLRAGELTPVWIPDLTHEAMRDLIRARAAAKRDSRVARQRILSMLLRTD
                     KRYAGKHWTGKHRTWLANQSFSQPSQQIAFQHYCQSLEQIEDRILQLDQEISRLLPEW
                     SLCNLVCQLQALKGVGQLTAITLVAELGDFSRFSSPKQLMAFLGLVPGEYSSGNSIRP
                     RGITKVGNSELRRLLYEAAWSYRTPAKVGAWLIYYRPDSVTQYSKDIAWKAQQRLCSR
                     YRSLTAKGKKSQVAITAVARELTGFMWDIALAAQSSFSQQKQN"
     gene            complement(73163..73633)
                     /locus_tag="ACHKA1_001054"
                     /pseudo
     CDS             complement(73163..73633)
                     /locus_tag="ACHKA1_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            73901..74140
                     /locus_tag="ACHKA1_001055"
                     /pseudo
     CDS             73901..74140
                     /locus_tag="ACHKA1_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            74438..74626
                     /locus_tag="ACHKA1_001056"
     CDS             74438..74626
                     /locus_tag="ACHKA1_001056"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678745.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            74954..75148
                     /locus_tag="ACHKA1_001057"
     CDS             74954..75148
                     /locus_tag="ACHKA1_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678746.1"
                     /translation="MNENRIKRLEQLLQARQREFATKWGRAALPHERLIYFPLEDLIK
                     NNGKKIRPDKKHFLECNEDK"
     gene            complement(75947..76271)
                     /locus_tag="ACHKA1_001058"
                     /pseudo
     CDS             complement(75947..76271)
                     /locus_tag="ACHKA1_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        20240 a        19129 c        18699 g        18326 t
ORIGIN      
        1 aaaaaggatg atgtcactat cattgagttg agacatattt attctgtatc caaaattttg
       61 gcaaaaataa gttacgggtt acaacgattt tcgaatcgtt ctttccctga tttttgtggc
      121 ttttgcttct gcttgcgaca gcagtccttg ttcctgcttg agaatgtctt taagcggggc
      181 ggcaaaatac actttcataa accgccagat atcccgctga gtcaggccga tattcatcga
      241 tgaaaaataa agcccaataa gatctttatc gcgccaacga cgtggaacgc gcgtgcgaag
      301 ctgcgcccgg tgcaggtcaa ttaccgaaat ttttaactcc tcttccttac cggaaaaagg
      361 caagtgcagc aggaaatgac agatataaca gtcacggtgg ttaatgccca cagcatgcat
      421 attgcgcacc atcgtcgcga cacgcttaat aagcatacgc tttacgcgaa catctgaagg
      481 gttagtcgcc cagtcagcac agtaatcttc cagacttatg gttggtgtca gatcttcggt
      541 aataataaat gaagttctgg tcagcggatt catgcctttt tcgccaaatg ccaccccata
      601 catagtatca acgccgacat cccgcagtcg atgaatcgca ttccattcgc ggtctgcgcc
      661 taatactggc atccgcaatg agagtaaatt tttgattatc tctttcaggg tcgtgccgcg
      721 atgccatttg agaaaatagc ttttgcccgc catttcaaag cgcagagtac ggcgagtttc
      781 cagttcacga aatacctcac cctgcaaggt tttaacttcc tcaaaaggat ctttaccgcg
      841 ccataacgtg gcaaacggct ctttaagttc aaccatccag accacccgtt atgatatccg
      901 ccgctttctc tggcagactg tataaatctt gtgtatcagc ataatgtcgc gcattttccg
      961 cccaggcctg gcgcaatgaa gattgcgtta acgctttgcg taaaatctca ttcaatgttt
     1021 cctggcggaa tggctcaaca atagcctcgc cgcaattggc ctcgacaata taatgcgcat
     1081 agccacaaac ggctgttgtt agtaccggta atcctgcagt aatcgcttcc agcagcacaa
     1141 ttcccgccgc ttcctggtag gcaggatgca gtaataaatc cgccgccgcc attaattccg
     1201 agacatcgtt gcgccccgag aagaagtgaa cattactgcg cacgccgcgt ttttctgcca
     1261 gcgcctcaaa ttttcgcggt ttatcctgcc caacaacata tagcaatgtg ttgtggcgca
     1321 gcgaatccgg taacgaagca agtgcttcaa tggaacgatc gacaccttta cgcgtgaagt
     1381 ctgaaccgac ctgcaacaat aaatattgtt gttcggttat tccattcttc ttacggaaga
     1441 tttcacggct attggctggc tgctggctat atttacgatc aggataaatc cccggtggca
     1501 gaatatgaaa acgctccgct tcagtctgat aatgtttctg gaaatcggcg atttgcttat
     1561 ctgtcagcat cagcagctgt gtcggcttgc cctgttcgaa ggttgcccgc tcaaaggcgg
     1621 cataatggcg ataacgtgac gtcaggcgat agaaaaagcc tttttcctgc gctactttct
     1681 cggcataaca aacatcagcg gcataataaa cgtccagccc cggcattttg ttgaatccaa
     1741 cgactttatc gacgggatgt tcgcgtaaat gtttttgcac ccaggcaaaa tactccgcat
     1801 tgcgcccgtg attggtatgc gatttaaccg gcactttgat cagttcaaat acatcagggc
     1861 attcgccttc ccacgactgg gtataaaccc gaacatgatg acctcgggcg gcgactgtct
     1921 gagcaatacg cataaaatcg cgctgcaaac cgccaaaggg aaaatattta tataaacaaa
     1981 aagcaacgat cataacgacg tacctgtcgt ggaggagggc aataatttat cgacagcagc
     2041 aatgacatct gccgccggaa taaccgaaag atacatctca tttcggtcac gctgatcgcg
     2101 cgttggcatt tcccggtaat ctcccgccca gaattgaatc atgttatttg accagggacg
     2161 ccagaaaata tggtctgtcg caccaaacag cgatatcagc ggcgtattaa ctgcagcggc
     2221 aatatgcgcc ggtgcggaat caacgccaat aaacagctgc gcatgatcga ttaacgcacc
     2281 aagttccggg aaggtcacct ttccagccag cgccgttact ggtggcgtct ggcatccctg
     2341 cgcaatttca ttgacgcagg ccagatcgtc tttatctggg ccggacgtca gaacgacttc
     2401 ataaccacga gcatgtaagg catcaatcac agcggaaaac ttggcgttgt cccagcattt
     2461 gaagatttgc cgcgccgtag gttggataac cacataattt tgtccaacac cagcgtgatc
     2521 aagttcgcga cgcatacgtt tccagcttgc aggcgggtaa ctcatggttg tctgcttcac
     2581 caacgaatca agtcccaatg atgtcagcac ggataagtta ctttccacca catttccacc
     2641 ctgcaacggc accaaatggg tgaaactttt acgccaaaaa gcagactgcc gatgatgata
     2701 atcctgggaa attttcacac gggcatttaa taagcgaacc agtatagcaa ccatccattg
     2761 atcggtaaga ttgacgataa ggtcatactt attggcacgt aataccttga tgagatgaaa
     2821 aaagttggca attttttctg aggcttttgc ttttttattt tttatgccgt agagcgcgtt
     2881 aatctctgga ttttcagaca ggatcgggat ggtgtcctga taaagcagca catcgatttt
     2941 tgcgtcaggg taattttttt tcagcgaact aatgacggga gtagttaata acatatcccc
     3001 atgaaaacgc atcttaatga gcaaaattct tcgaaatggc ttatccacaa gtaactcttt
     3061 ttgttgagat tatccgatta aggtgaagca gaaaaagcac gctaccgccc caggctcaac
     3121 agctacctga atactgatac cgcactgata ttgacgcgct agtgtatcac ttctttcgcg
     3181 gcaatccgac ccgaatgatt tttaagcaca ttgttgattt tcaataaaga aatcaatcaa
     3241 ataaaatcat tagcaaccat attctttcac tgttgctctg cgattccttc ctgcacgtac
     3301 agcagaaatc aatgaatcgc ataaaaatac aaaaaaagta tagatgttca gcaagaattg
     3361 gcgggtaaat tgctatttaa gtcaaaaacg ggaaaagtaa tggtaaagcc acagctaaat
     3421 acatagaatc cccagcacat ccataagtca gctatttact atgctcgaat tgctttacac
     3481 cgcccttctc taccttattc agccgctgat ctggatacgg ctctgggtgc gcggacgtaa
     3541 ggctccggcc tatcgaaaac gctggggtga acgttacggt ttttaccgcc atccgctaaa
     3601 accaggcggc attatgctgc actccgtctc cgtcggtgaa actctggcgg cgatcccgtt
     3661 ggtgcgcgcg ctgcgtcatc gttatcctga tttaccgatt accgttacaa ccatgacgcc
     3721 aaccggttcg gagcgcgtac aatcggcttt cgggaaggat gttcagcacg tttatctgcc
     3781 gtatgatctg cccgatgcac tcaaccgttt cctgaataaa gtcgacccta aactggtgtt
     3841 gattatggaa accgaactat ggcctaacct gattgcggcg ctacataaac gtaaaattcc
     3901 gctggtgatc gctaacgcgc gactctctgc ccgctcggcc gcaggttatg ccaaactggg
     3961 taaattcgtc cgtcgcttgc tgcgtcgtat tacgctgatt gccgcccaaa atgaagaaga
     4021 tggtgcacgt tttgtggcgc tgggcgcaaa aaataaccaa gtaaccgtta ccggtagcct
     4081 gaaattcgat atttctgtaa cgccgcagtt ggctgctaaa gccgtgacgc tgcgccgcca
     4141 gtgggcacca caccgcccgg tatggattgc caccagcact cacgaaggcg aagagagtgt
     4201 ggtgatcgcc gcacatcagg cattgttaca gcaattcccg aatttactgc tcatcctggt
     4261 accccgtcat ccggaacgct tcccggatgc gattaacctt gtccgccagg ctggactaag
     4321 ctatatcaca cgctcttcag gggaagtccc ttcctccagc acgcaggttg tggttggcga
     4381 tacgatgggc gagctgatgt tactgtacgg cattgccgat ctcgcctttg ttggcggttc
     4441 actggttgaa cgtggtgggc ataatccgct ggaagctgcc gcacacgcaa ttccggtatt
     4501 gatggggccg catactttta actttaaaga catttgcgcg cggctggagc aggcaagtgg
     4561 gctgattacc gttaccgatg ccactacgct tgcaaaagag gtttcctctt tactcaccga
     4621 cgccgattac cgtagtttct atggccgtca tgccgttgaa gtactgtatc aaaaccaggg
     4681 cgcgctacag cgtctgcttc aactgctgga accttacctg ccaccgaaaa cgcattgagg
     4741 ttgttatgca aaaacgggcg atttatccgg gtactttcga tcccattacc aatggtcata
     4801 tcgatatcgt gacgcgcgcc acgcagatgt tcgatcacgt tattctggcg attgccgcca
     4861 gccccagtaa aaaaccgatg tttaccctgg aagagcgtgt tgagctggca cagcaggcaa
     4921 ccgcgcatct ggggaacgtg gaagtggtcg ggtttagtga tttgatggcg aacttcgccc
     4981 gtaatcaaca cgctacggtg ctgattcgtg gcctgcgtgc ggtggcggat tttgaatatg
     5041 aaatgcagct ggcgcatatg aatcgccact taatgccgga actggaaagt gtgtttctga
     5101 tgccgtcgaa agagtggtcg tttatctctt catcgttggt gaaagaggtg gcgcgccatc
     5161 agggcgatgt cacccatttc ctgccggaga atgtccatca ggcgctgatg gcgaagttag
     5221 cgtagcgttt atgccggatg gtatgccatc cggcgcgcat gaattacttc tggcactgcc
     5281 gacaataaaa cgttgcccgc tgcgcatgtt tagtcgccac aatcggcgta ccgcacaccc
     5341 gacagggctc gccttttcgc ccgtaaacct gcaattcctg cgcgaaatag cccggtttac
     5401 catcactttg cagaaaatct ttcagcgttg taccgccctg ctcaatcgaa cgcagcaaca
     5461 ccgctttaat cacccgagcc agcaattcgc actccgccag cgacagtgat gacgccagcc
     5521 gatccggatg gatccccgcc gcaaacagag attcgctggc atagatattc cctacgccta
     5581 ccaccagctt gttatccatc agccacggtt taatcgccgt ttttttcttc gcgcacttct
     5641 gatgcagata ctcaccattg aaatcgtcgc taagcggctc cggcccaaga tgagccagca
     5701 cgttgtgccc ttccagctct ttggtccaca gccaggcacc aaagcggcgc ggatcggtgt
     5761 agcgcagcac tttaccgttg ctcatcacca aatccacatg gtcatgcttt tcagggggaa
     5821 gttcttctgg aaggatgcgc aggctgccag acatccccaa atgaatgata atccagccct
     5881 caggcagctc cagcagcaga tatttagccc gccgctgcac gctaagcact ggttggtcgc
     5941 ttaaacggta gatctcttct gaaaccggcc agcgcaagcg tccgttgcgc accaccgcat
     6001 gaagaatggt tgcaccaacg agatgcggtt ctatgccgcg gcggctggtt tcaacttcgg
     6061 gtaattcagg catagcatct ccaggaatga acagatgcaa tcaatatggg ggtaagcaga
     6121 taacaaaaaa ccccgccgga gcgaggtttt tcgttacatc aaagcgagaa ttatttgatt
     6181 ttcgcttctt tgtagatcac gtgctggcga acaactggat cgaatttttt cagttccagt
     6241 ttttccggct tagtacgttt gttcttcgta gtggtataga agtgaccagt accagcagaa
     6301 gaaaccagct tgattttctc acgaatacct ttagccatga tttatttcct ctaagtactt
     6361 agtacttttc gccacgggca cgcagttcag ccagaactgt atcgatgcct tttttatcga
     6421 ttacacgcat acctttagca gatacgcgca gggtgacaaa acgcttctcg ctctcaaccc
     6481 agaaacggtg agagtgcagg ttcggcagga aacggcgttt agtcgcgttc agtgcgtggg
     6541 aacggttgtt accggtcacc ggacgcttgc cagtaacttg gcagactcgg gacatgtcta
     6601 ttctccaaaa atcaaattag ctcgagcttc gtatggggta ttggcgcctc gtcaggcttt
     6661 acagcccggt catcgcagtt ctatgtgaac tctcgattgc caggcccaaa tgccaaaccc
     6721 gagattctca aaggtggcgt agtatacgct gactcagcga tgtgctcaag tcccgaacag
     6781 acaaagatcc cgaaggatcg cgcatagcgg gttaaatcca gccgcgttcg gcaaaagaaa
     6841 catactctcc acgcccaatc acgatatggt cgagcacgcg taaatccatg aactgacaac
     6901 tctttattat ccgttcagta atgagtttat ccgctttact gggttcagca caacccgaag
     6961 ggtgattatg tgcaaggatc agcgccgagg cgtttatttt tatcgcttcg cggataattt
     7021 cccgaggatg gacttcaaca tggtttagcg tgccggaaaa aagacggcta tgcgttataa
     7081 cccggtgttg tgagtcgaga aagatcacca taaagatctc ccgctcctca cccgtgagct
     7141 ggctttgtaa aaattcccgc gtcatctccg ggctgagtaa agggctttct tcacgcatcc
     7201 gcacgttgta gtaacgccgc gccagttcag caatcccctt taactgggca aatttcgcta
     7261 cgccaattcc atgaacacca ctaaattgtt catattcaga ggttaacaag ccataaagag
     7321 agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg cgcgttccgg
     7381 tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca aacttcaaca
     7441 ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc tttgtggtgc
     7501 tcgcatcctg tcatagccgt tccgttaaat cgacggtcag ttttcaatcc tggaaagcgc
     7561 ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata aaatcgccaa
     7621 cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa aaatcgttct
     7681 cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc gtttgcgcga
     7741 tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta tcaccccact
     7801 tagcttgcag gcggtttctg ggtatcccgt ttccgacagt ctgcttgacc cggcagccga
     7861 agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc tcgcccccgc
     7921 cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg tatcgacgat
     7981 ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc agatgtaccg
     8041 tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc tcatctgggg
     8101 gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc tcgatccgtt
     8161 aaccattgtg gatatggcag tagcgcattt ttcgcccgtc aacgacctga aacatctgaa
     8221 cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt atatctctaa
     8281 tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc gtggcgcgaa
     8341 cgtcacgctg gtatcaggtc cagtttcact accgacgcca ccgtttgtta aacgtgttga
     8401 tgtgatgacc gcgctggaaa tggaagccgc cgtgaatgct tctgtacagc agcaaaatat
     8461 ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc cagaaaaaat
     8521 caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa accccgatat
     8581 cgttgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat ttgccgccga
     8641 aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc ttgatctgat
     8701 ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca acgcattaca
     8761 ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc tccttggcca
     8821 attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt aagattctgg
     8881 acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc tctgccggac
     8941 ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac actacgctgg
     9001 ttccgaccgg gctggcaatt catattgccg atccttcact ggcggcaatg atgctgccgc
     9061 gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga ttgatcgatt
     9121 ccgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac agcttcacca
     9181 ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag gctgaattta
     9241 atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt cactctggtc
     9301 gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat ttcgtttgcg
     9361 gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg taacatggca
     9421 gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct ggcgctgatg
     9481 ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc ctctgtcggc
     9541 gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt cgatagcctg
     9601 attgagttta tcgaagatag cctgattact cgcatcaatc tgattctgaa agatgagaaa
     9661 gacaccacag cgcgcctgcg tctgatagtg ttgctgcttc tcggttttgg tgagcgtaat
     9721 cctggcctga cccgcatcct taccgggcat gcgctgatgt ttgaacaaga ccgcctgcaa
     9781 gggcgcatca accaactttt cgagcgtatt gaagcgcagc tgcgtcaggt attgcgtgaa
     9841 aagagaatgc gtgagggtga aggttacacc actgatgaaa cgctgctggc aagccagttg
     9901 ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa atatcgcccg
     9961 acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta atatgacgcc
    10021 ggatgacttt tcatccggcg agtttcttta aacgccgaac tcttcgcgat aggccttaac
    10081 cgccgccaga tgttccgcca tttccggctt ctcttccagg taggcaatca gatctttcag
    10141 ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa tcgccgaaat
    10201 ctcgccgcgc ccgcgttcct gacgatcaag cgaaatcaat acgccagcaa gcgtcgcgcc
    10261 attggcctga ataatttcca tcgactcgcg aatcgccgtt ccggcggtga tcacatcatc
    10321 caccagcatt acgcgtcctt gtaacgcgct accgaccaga ttgccgcctt caccgtggtc
    10381 ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct ccgccagcgc
    10441 cacggcggtt gtggtagcaa tcgggatccc tttgtaagca gggccaaaca gcagatcgaa
    10501 ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg ccagatcgcg
    10561 cccggtatta aacagcccgg cgttgaagaa ataggggctt ttgcgcccgg atttcagcgt
    10621 aaactcacca aactttaaca cctgcttgct aagcgcaaat tcaataaact ggcgctgata
    10681 tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag gcgactcatc
    10741 agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat ggattcgatt
    10801 ccccctcggg ccagcgccaa caaggtgagt aactcttcat gggtgaacgg ctcgccttct
    10861 gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt catgtcggtc
    10921 tctgcggctg agtcttcaac gtattccaga tcgcaaattg cttcgccgtt aacaataccg
    10981 acagaaaccg cggctaccat ccctttcatt ggattggttt tcagcttgcc gttttccacc
    11041 agcttctgta acgcatctgc cagcgccacg caggcacccg taatcgaagc ggtacgcgtg
    11101 ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc cagcgctttc
    11161 aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat tgtacgtcca
    11221 ccctgctttc ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg tggcagcatg
    11281 ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg cacgccttct
    11341 tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac cgagccttct
    11401 gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt gctacggcct
    11461 gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt atacggactt
    11521 ccggcggtta ttcctatcct gacaaggcat cgatggctat aatccttcca cctctccttt
    11581 tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt gaaatcaagg
    11641 gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat ctggaaactt
    11701 actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgtgag cgtattcgtt
    11761 ctcgcctgac acgcggtaaa gtggaatgta ccctgcgcta tgagccagat gttagcgcgc
    11821 aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc gcgaactggg
    11881 taaaaatgca gagcgacgaa ggagaaatca acccagttga tattctccgt tggccgggcg
    11941 tgatggcggc ccaggagcag gatctcgacg cgattgccgc tgaaattctc gcggcgttgg
    12001 atggcaccct ggatgacttt atcatcgctc gcgaaaccga aggtcaggcg ctgaaagcgt
    12061 tgattgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc tcccatatgc
    12121 cggaaatcct gcaatggcag cgtgaacgct tggtcgcgaa gctggaagat gctcaggtgc
    12181 agttggaaaa caaccgtctg gagcaggagt tggttctgct ggcacaacga attgacgttg
    12241 ccgaagaact ggaccgcctc gaagcgcatg ttaaagagac gtacaacatt ctgaagaaaa
    12301 aagaagccgt tggtcgtcgt ctggacttta tgatgcagga gttcaaccgc gagtcgaaca
    12361 ctcttgcttc gaagtctatc aatgccgaag tgacaaactc cgcgattgag ctgaaagtgt
    12421 tgattgagca gatgcgcgag cagattcaaa atgtggagta agctatcaga ggaaatcaat
    12481 aaagcgcaat aatccaatca aacccgcgaa aatgcgggtt tttttgtaat taatgactat
    12541 cataacgacg caagaaagcg catgattagc gtgtacataa aggtgtacat aatgccattt
    12601 atgtacatcg ctttatgtac acacctctat tcaatgttta gggatgcctt aatgccaaaa
    12661 cttacagata ttcagatccg tgcctggata aaggcagacg agaggtttga cggcaaagct
    12721 gacggtaacg gcctgtatct ctgctatccc aaaaattaca caatgccctt ctggcgattc
    12781 cgctataagt ttgctggcaa acaacgggcg atggttatcg ggtcatatgc agagttatca
    12841 ctatccagag ccagagaaac agcaaaggaa ctatccgctc gcgtcgcact tgggtacgac
    12901 gtagcagcgg agaaacagga gcgtaaagcc gaagcactgg caaaaatgga ggcagaaaaa
    12961 aacgccatac gagtatcaga gcttgctgcc gaatatttcg aacgtcagat actgccacga
    13021 tggaaacatc ccgacatcct gcgccgtcgc atcgacaaag acataaaccc gcgtattggt
    13081 catatgaagg ctgaggacgt aaaaccacga cacattgacg agatgctaaa ggcaattgtt
    13141 gaccgtggag cgccaaccat tgcaacggac gttctgcgat ggacgcgccg catattcgac
    13201 tacgggataa aacgtcatgt gctggagatt aacccgtgct cggcgtttga agtctctgat
    13261 gctggtggca aggagtcatc ccgcgatcgc tggctgaccc gtgaagagtt aatccggttc
    13321 tttcaggcca tgcgtaccgc aaagggattc agccgacaga acgagctaac cttcaagttg
    13381 ctactggcgc tttgcgtcag gaagatggag ttgtgcgccg ccagatggga agagttcgac
    13441 cttgataacg ccgtgtggca tttgccagag gaacgcagca aaaacggcga ccccatagat
    13501 attccacttg ttccccccgt tattgaatgg ctgaaagaac tgcgcgtctt gtcatgcaat
    13561 agcgcgtggg tacttccggc gcggaaaatg cagcacagga tgatccccca cattcaggag
    13621 agcaccctac ccgttgcact ggcaaaggtc cgcgcagaga tgccggaggt tccagatttc
    13681 actattcacg acttccgacg tacagcacgc acacatttag ccgcgcttgg cgttgatccg
    13741 gtagtcgctg aaagatgcct caatcaccgc atcaagggag tcgagggcat atacaatcgc
    13801 tatcagtatt tcgatgaacg gacttcggcg ctggcgaaat ggaccaatct gttagtgtcg
    13861 ctggaacgcg gggaacgcta caacgtgaca ccaatcagaa gggccaaagc atgattaata
    13921 tcctgaaaaa atcgccaata gaacgcctgt gcgcttctgt ttcaatcact ccgcacgaaa
    13981 tggcccttgc actggctggc cttaatccgt caatgagaat aggcgacgtg ccgcaggaca
    14041 agttcgaaca ggtcgaatca gccagaacga tgatagcccg tgctatctgg ttgcatagcg
    14101 gcaagaaagc gggaaaggat gagccataca gagcggggga tatattcctt gcgtccttcc
    14161 cgttcattga ggcaggaacg ccagaggcaa taattacggc ggtaactgat gccattgatg
    14221 atttacgagg aacaaaaaat tgggaagaaa aagcacttaa tcttggtggg cggcggctgg
    14281 tcagccacat caaagaaaca agccgcagcg gaagggggca gtacaggaaa ctcgacgaag
    14341 aacagggaaa catgaagatg atggggttgc tggttttact gctcgtgaag aaatcaggca
    14401 caaccgcata cattcaggac ggagaaccaa accgttcagc catttaccgc gacgtggaag
    14461 cgctgatgaa ggagaaaggg atatcgccaa aagggatcgc caaatccacg tttatgcaaa
    14521 aaatatcagc cgcattactg gcagtaagcc aggcagatta accagccaac acgccctgta
    14581 ttcacggggc tgtaatcccc atacatcccc caccagcaaa atttactcac cgttcttaat
    14641 cgttataaat cggtataaac aggattagca cgttaaagac tggcaaaaga tggcaaaaca
    14701 taacaacgca taacggcgca gtaggagtat tacggtatca gaacgtatac ggcttcaatg
    14761 acttatcacc aataaagcaa aaactttcaa agttttagca ctccatcccg cctgttcaat
    14821 cttttactgg aagtgtgttt tttcaataga tttgtggaaa gttttaatag attccagatt
    14881 tggtggaatg ctgtacaaac aaccaataga atggagaaaa catcaacttt taacccaaga
    14941 gggaatcatg acagctaacc atcaagaagc tatccctacc acgggctata tccgccgctg
    15001 caacctggct aagttgcttg ataccagcgt gtcaactatc gaccgctggg tgcgtatcgg
    15061 caaattaccg aagcccacga agctggggga gaaaaacacc gtatttaatg cggtggagat
    15121 caaccactgg ctgaacgagc gccgcaacaa caccaagcag taatcagcat gacgggcgta
    15181 cagttccgcg cccgtcagca ccaaacctga tccgatagca ggagttttca atgaaacgtc
    15241 cgaatatgcc cgtacagggg cagggcttcg ttcagcctga aatcagccag aacgatatat
    15301 tgattcgcca caaagcggag tcgcgtattg attcccgcgt tatttctgag cgagcaggaa
    15361 tacaacacga aagtatcatt gcgacgatta aatctcatca atccagattg cgcgagcttg
    15421 gctcactgcc tcgccagtca gtcaaggaat taacctattt gaaatcggga aaatctggcc
    15481 gcaagcgcgg tcgtcctgaa attagttatt tgctgaatga gctacagctt gactatctgt
    15541 tacgcgttat ccgtgggcgt gacgcagaac gtatcaacca gttcaagctg gatgttacga
    15601 aggcattcgc gaagcatcgc gccgctgaac cagtccgtcg cgaatatctg ccaggctatc
    15661 acgaaagccg tgacgggctt aaagcactgg gcgcagaacg tcaccattac atcaatctgg
    15721 cacgcgcaga gaaccgtgtt acaggtctgg tggctggaga acgcagcaca gccagcgagc
    15781 aacagttagg cttactgata gtcatgcaga aaatcgagca ggcagcattt gacgaggcga
    15841 tacagagcga catgacaccg accgaagcgg tgcgggaagt tgctcgccgc atggagacgt
    15901 tcgccagcct gatgacgtca gcgtcgtctc tgggggtgaa acatgcttaa gaattttgcc
    15961 gaattaacac cggaacgagt ggagtttatg cgccatatcg tgagccattt cgccgctgag
    16021 cgcatgaaac acgttaagca atacctggag gaggttttat caaattatcg cgctggtggc
    16081 cctgtacggc gcgaatattt gcatgatgca ggaatggcag gcaaaacaaa ggcgaccacg
    16141 gaagggtcgc cagtggagat aaaggaaatc aagcgcatca ccaactatgc catgagtgta
    16201 gctggtggac aatgctatca gcctggcggt cggtttttag tggcttttaa ccgccgtggg
    16261 tatttctcga caaaatcttc aagggcaaat cgtttttgtg ggtgtttttt cactccggga
    16321 agaggcgtta actcttcggg ttctgatatc tctttagcgc gtttcagttc cagttggcgc
    16381 tcaagtatgc ggtgcaaaag ctgtcttcgt tgttcggcaa aaatatctgt aatggattgg
    16441 tactttttgt tcatgctgct gccctgtaaa gcaatgcgcc gtagtacctc acaccacggc
    16501 gctgatagtg attattctga ttctttggcc ttgcgacgct ggcggtattc aacttcccgc
    16561 tttaatgcag ctgtaacaaa ctgccctgta ctttcgccgg gcatttttac cgcctcaaca
    16621 ttgttcataa cttcatgtgg aacccttgcc gcaactgttt gtgattttgc gtttactgct
    16681 tttgtcgcca tgttgagcac ccctcataaa aaacaaatgc agtatgcagg aaaaaaaaca
    16741 agtgttcaac acttgacgtg tttaacacct cgatttaaat tggtgttcaa caccttattt
    16801 aggcaaggtg tagaaacgac gaaaccccgc actgtaggag cagtaacggg gcttctaacc
    16861 aacaacgtaa acgaggagcc gttatggttg ccgtaaatca tataccacac cttgtacaca
    16921 cacaaactgc ctttgtgtgg cgttttctgg cactaagtgc cggagaatct caaatcatcc
    16981 acgtaaccgc ctggacggaa cgcgaagcgc gtagccgttg cccgtcaggt tgcgttgctg
    17041 tattcgccgc ccgtatccgt caggaggcat cacatggata aaaacatgtt gatcgcctct
    17101 tctttcgggc tgtatgatgc cggagtcgcc cacatggcga ccgggtttga cagcctgaat
    17161 aaattggcgg acagccgccc atatccgata agcggttttt ttgtgtccgt aaacctaccc
    17221 atacccgcat tatggcgggg cgtaacgggg gagcctttgc gctcgctggt ttcccaattt
    17281 gccagtctgt caaccctgtt acgtctcgcc accatgtttg acagcgtagt agcgagactc
    17341 cttaacatta aattgggagc ctttcacatg gctgtatccg cacgccctta ttttgtctgg
    17401 cgctttatgc agtgcctgac agacagcatt gcaatattca ccgttaccgc tgccactgag
    17461 cgcgaagcac gcgcccagct gccgcacgca catctgattt ttgtcgcccg aatccgtcag
    17521 gaggtgactc atgcttaaaa ccttccgcgt cttttcccgt gctgttaaca agcgtggcta
    17581 taccgttggc atcacgcaga acgtgaaggc cgaaaacgtc caggctgcaa tcagcacagt
    17641 aattaacaaa tcagctaaag ccggtttatc cggcgtaatt atccacgcag tgacatgtat
    17701 tcaggatgag gagtaattca tgtgtgattt ttccgatatg actgttgatg aagcagcaaa
    17761 aagcgcaatg caggcagaac ttatttgttc tctgatgatg ggtaacacag gtggaatgac
    17821 tgagggtgag attgagtcat tgctcgccct gattaagcag cttacaggta aggcaggtag
    17881 ctggctgatt gctgcatcgg gtgatgatgc aatatgcgtc aatgactgaa tcctttgttg
    17941 ttctgaatat catgaaatta agggggtgca gtaatgcagc aaataacact ggcagaagcc
    18001 tcactgcaag cgcaacaggt cgaagtaatc agccgcatga ttgaacgcat gattgaacgc
    18061 aaccacgaag aaatagacga ccatgacctt tctgttattg caggacttat caaacaactt
    18121 tccagcaatg tggcggtgtg gttaatgacg gaggaagaaa aacgcggcga acaataacgg
    18181 ctaaaaataa acgggaaata aagattaaga cgactccttc aggagattaa ttcaatggca
    18241 tcaaaaaata ctggcttaac tgccagtagc aaaactcacc ccgaatttat gtgcggggat
    18301 atttaccgca atgaatacgg tagccacatg acgatgatca gcagaggtaa gaaatgaaaa
    18361 gcgcaccgaa cttaaaaaaa cagcctcgag ggaaaaaaca cgccgatacg gaagtgatta
    18421 ttttcgctgg cagtgatgcg tgggcgcacg cgaaacagtg gcaggagcag gacggaaaac
    18481 ttgccggaga taaaacaccg cctgtcgtgc tggctgatga gcaacttaaa gatattggca
    18541 atctgcaaat tgtacccgac gggcgcaaat cagcgcggat atacaaggcc ggacacctcg
    18601 atcaggtgat ggtcaaaggc ataggtcaga agctggcggc ggcaggcgta caggatgctg
    18661 attactaccc cgaaggtatg caccataacg agcggcagaa ctggcgtaac tatctggaga
    18721 ctgagcgcaa aaacatttct gacggactgg ttattgagct gcctgtaaag aaaaaaacat
    18781 ccgaacaatc agaggatgaa ttaaaacccc gcgttgaatg ccgtcctgat ggcgtttatt
    18841 gggttacgcc aaaggttgat aagcaatctg gcgaaattat ccgcccgtgt gagtggattg
    18901 gtgaccgtat gcgaaccgtg gggatcggcc atgatggctg cgatgggtat ctgataatcg
    18961 aacttgaacc agagggaatg gaaaaaatta tttatgaggc catgccccgc aatgaaatcg
    19021 gcctcccttc tggatggtcg cgattgcgtg ggtgcggggt ggcagtgaca accagtcaga
    19081 aacttttaaa caaacttgcg gaataccttc agcgtgaagg agagcggaca cagtgggagg
    19141 tcacatcaac ggcggggtgg cattgcggca cttatgttat gccggatggt gagattattg
    19201 gcgtaccaga aaggcccgtt gcattctgcg gtggttcggc ggcgattaaa ggttatgtgg
    19261 ttcggggtac ggctgatgag tggcgcgaca atgttgcctc actgatgcgc ggtaatcact
    19321 caatgatgct tggagtgctg gtcgggcttg ccgcccccct gaactccctt gttggtggcg
    19381 gctgttttgg tgttcatctt tttgcgcagt cgtcggcagg aaaaaccacg acagtggaag
    19441 cagcaacaag tctttacggt gatcctgaag tgctgaaact ttcatgggat gcaaccagat
    19501 acggcctcac ggtggaagct gcggcacgta atgatggctt tatccccatt gatgaaatcg
    19561 gtcagggcgg acggattaac gatattgcgc agtcagccta cagcctgttt aacggtgtgg
    19621 ggcgtattca ggggcgtaag gatggcggta accgtgcggt gatacgctgg agaatcgcgg
    19681 ccctgagcac gggagaggag gatttcgaaa ccttcctgat taagggcggt gtgacgccta
    19741 aagctggtca actggtgcgc ctgttaagtg tgccgtttgt tgatacaacg gtttttaacg
    19801 gatatgacga tggtgatcag cacgccagag caatcaaacg gtttgcatcg aattattgcg
    19861 gagcagcggg tcgtgagtgg atcggctggt tgtcagccaa caaagaactt gcggtcagtg
    19921 tggttaacga aaaagaaaac atctggctta gtcgtcttcc tgaaaatgct tccccccagg
    19981 tgaaacgcgt ggcatcccgt tttgcaatgc tggatgcagc cggagaactg gcaacaggaa
    20041 taaccggatg gacagcggga gaaagccatg tagccacaca gcgcgctttt gatgactggt
    20101 tgcaggattt tggactggat aacagggaaa aatatcaggt tatcagtcgc gcccgcgatt
    20161 tcattcagcg tcacgccctt agccgttttc agccgtacac atacggcaga cagaacggag
    20221 atatggacgt gaattacgga gcgagaatca ccagtctggc ggggtacctg gtgagagggc
    20281 gacgtgatga cggattaccg gaataccaca tcataccgtc agtatttgat gaagaaatcc
    20341 tgtgtggcat taaccgaaat tttggatgcc aggcactgaa agaagccgga attctgattc
    20401 atgcaggtga taaaaactgg acgaccaaaa cgataaaggt taacggaata cagcaacgct
    20461 ttattgtgct tcttgaccag ccggaggagt aaaaaatcgt attctcgtgt acgcgtaaaa
    20521 aagtgggcgg ataaggcgga taagcggata tggttaataa agtatttata tatcagtgca
    20581 ttatgttgta tttccgtatc cgcccttatc cgcctgtatc cgccctgtta tgagaggaaa
    20641 ttgataaata tcaatattgc acaaaaagca cacaaccccg tatccgcctt tatgtttttg
    20701 attctaaaga aaaaaaacat gcagggcgga tacgggcgga tacgaaaaat cacgtaacac
    20761 catgaataca aacaatattt ttcttcatat ccgcttatcc gcccaaaacg cgtctttata
    20821 agtgaatatg tgaagttaaa aaacagagag cagagcggga gattaatgcc atttttcccg
    20881 cagtaaccac agagaggaat cgagtaatgt gcaaagaaaa aaacttttac cagtgcatga
    20941 gcagcagaga gcgcgaagac ctgaaaagtt ttgtgacacg gtgcgagatt cgcggagata
    21001 ttcagagcct gagcaggaca ctgatcatga ttgcgcactg gatgcgccag ggcaaccccg
    21061 tgaacttcac agaatatgcg agtcagtgga cagaggcaca actggaacgg gacgacggta
    21121 atcactcaac gccggaaatg gcgcagcaat ggcctttcag tggcaagcga tgtattcgca
    21181 aggggtactc tgattattat ccgttcggcg ttgatggttc acgtcaggat gacgaaacgg
    21241 aaattaaaca cgctgtaacg gtaattctgg cctcatggcc tctgtttaac cgtgacgggc
    21301 ttgatctgta caaccataac aaagcctggg agcacccgct tgattatgtg tgtttcatgg
    21361 aagaggcgaa aagctgttta cgctggataa gggagaacag cctcacaaat gacaagatag
    21421 cgtcattccc tgagaaaaac ccgacttcat acgggcttaa gcattgcgtt gaacgggcaa
    21481 ataagagact caccctggaa aacggaatgc cgacggaacc aacatacatt tcaaatggtg
    21541 cattcatcgc ggcaatggtg gcatccggtt ataagataaa accagcaggc cggatgaatt
    21601 gccggttcaa tatttcgcat aagcaactta agcgggttct gagtggtgaa ttatataaag
    21661 ccggagagtg gacaatatga ccgcacaaat tgcagcttac gggcggctgg tggacgaccc
    21721 gcaggaaaaa cagaccagca agggaacagc gatgacgctg gcgcgtatgg cggtatcttt
    21781 gccatgcagt caggcacagg acggacaggc gacgttatgg ctatcagtcc tggcatttgg
    21841 taagcaggcc gacgcgctgg cgagacatga aaaaggcgac cttatcagcg tggcgggtaa
    21901 catgcagatc agtcagtgga ccggacagaa cggagaaaca cggcagggct ggcaggttat
    21961 cgctgacagc gtaatcagtg cccgttcggc gcgtcctggc gggaagcgtg ggcaacaggg
    22021 gaaggcgacc gaagcactgg aaagagcaaa acgacagacc ggacagcagg gaaatcagcc
    22081 accagcggga ggtgatgacc cgttctctga cgatattcct ttctgaatga caggagcaca
    22141 acatgaacca gcagaccaca caacaggacc agaccacacg cagacccacg gagcgcccat
    22201 catggcgaca catgacgaag cggcagcgaa aaaaccgcag gaggataaac cgactggctg
    22261 agtactggcc tgaactgttt ggggcagaaa ctataaagcc cctgaaaacg ggcataatac
    22321 gtgatgtgct tgaggacgct aaatcacggg aattaccgat tggtcatggc gtattatgtg
    22381 gcgcattaac ctcatacatc cacacgacgc gatacctgaa agccattgtc gctggtggcc
    22441 ctcggtatgg actgaacggg cagccttgcg gggaagtgac gtcggatgaa aaagccgtag
    22501 cccgtgggat actgaaacag cgactggagc agatgaagca gaaaaacatc cagcagtaat
    22561 cagccggagg taaagccagt gataacggac agcgtggcac agaaacttga ggagcgcggt
    22621 ttatggcgac gtgcggcgac ccgatgggct gaggttcttc ttcacgcaga aacagacagg
    22681 gaacgcgagg aagctgccag acgtcgggcg atatgtatca ttaaatcccg tcgtatgcct
    22741 gaacaactgg tcacctttgg cgatgtgaaa aaggccgcag acagaacgct gaaagagatg
    22801 gggattaatc cacaggatga atggagaagt tacagcttca gtgatgcagg cgacgattta
    22861 acgctaccgt gatacgcatc tcatcgccgt atttgctcag ttatcgcgat tcgctataat
    22921 caccaccgaa aggccgttga tggtgttcgc ctcgggcaac tggagcccga ctgaatgaag
    22981 ccagccagcg gcttttcggc actcattccg gtgtatctcc catacatcgg gatgagtgaa
    23041 cgacgaaaac atcacggtga tttcgtcatg ccgcgataag aatgcgcctc tttgggcaat
    23101 tgaagccctc tgtacagcgg gttatcgcgg caaagttgtg ataaaaacgc gcctctttga
    23161 gcaactggag ctcactgtaa agcggcttat cacagcaggc cgtaacgggc gctcatcttt
    23221 gggcaattgg agccctctgt acagagaccc gttgcggctg accccgaatg attttgcccg
    23281 gggcaattga agcaccccgc aaagtgattc ggggtttttt attgcccgtc caccagctgc
    23341 acacctgtag cattgatgcg tatacattcg ccgtcaattg caccgttccg gccctcaccg
    23401 tcacgcgcaa caacagaacg acctggcagg ccataacgcg ccacctttcg acgaacgcca
    23461 tgaccaacgc tgtcacgccg ctcatacaca ttaacccgct caagaagacc atcacgcacc
    23521 attgcttcaa gcgtgcgcct tgttgattcg agctgatatc ggttgttgtg tgaatccatg
    23581 ccatacagca ggtaagccac accggagaca tcaaacggcg gactgccaat ttcggcagta
    23641 acccacgcca gattgtcagg agtgaagtaa ctgaggatct cttttttgta ttgcgtcagt
    23701 ctcatagtga cctttcttgt tgtaggatga atccctcata caataagcca taaggcaaat
    23761 tttgaaagga cagaatcggc ggggtacaga tgatacgtga tgacaaggct gaagcgctgg
    23821 agaatagcgg cctctggcgg cgtgctgctg aacgctgggg agaattgttc agacaggcaa
    23881 aaactgatga agagaggcac tatattaccc aacgaagggc gcaatgcacg gcaaaagcca
    23941 ccagaccaaa aacaacagag tgggggcatg tatgcgccgt cagggcagca gcgaccagaa
    24001 cgcttaaaga tatggggatt gaccagaaaa aagaagatcc tttacggggc acgaagtaca
    24061 ggggtgaaac agggcgaaga gacggaagtt aatatggaca caataactga taaaaaagcc
    24121 gaacaactgg aatctcaggg actatggcga agagccgccg ctcgttggct tgacgtaatg
    24181 aaagaggccc acaccgatcc gcagcgagag catatagcca gacggcggga aatatgcctt
    24241 gcgaacttca ggatgctgta aataagactg ttgacgatat gggaatcaga cgggatcggc
    24301 gacattacgg gttatggaaa taacagccag gtaaattggt gaatatacgg caggctgcaa
    24361 tcagagccga acatagcggc ctccgtcacc attatcagac agtagcggta gtccttccat
    24421 cacagcaaaa tgctacccac ttcacagccc cttggaagcc tcttcgatgg ggtgaaaaat
    24481 ccctgaaaaa atgcgcaaaa aactgcgcaa aaaagtgcgc aaatcgatca taaaaccgat
    24541 cacaaaatcg atcacacaga caaacagagc aaagccagat atgacgcggc ttgcaggggg
    24601 aaaggccacc ggaaaaagtg cgcaaaaaaa tgcgcaaaac gaaacgcaac agccgcgcaa
    24661 aacgaaaccg atacgcgatg atcgaaaaag cgcgcaaatt taccggattc gggtaagcaa
    24721 ttttgcctga tttgggcaaa atacggtgac gccgtaacag tgacattgac accaaaacag
    24781 tgacacacag gagggccgat gaccgaacgt tcaccaacag agataattca cgggattatt
    24841 gaagagcagg aggaacgcga gttaatcctc atgattgaag agcagagaga acgccagcgc
    24901 aaacaggagg ccacgcagcc ggacagcgtg agcgctgaaa atcaggctga tgtgacgtga
    24961 tggtgtcgaa ctgagtagct ttaagcgact ctgtgactgg ttttaaacga gtcgcaaaaa
    25021 gagccggaga aaaaccacga atctggggtt tttactggcg cggtgtacac ggtggaacgc
    25081 atagcgttac ctgacttatt gcattgtaaa aagttagaaa ggcacggcct gtaagatggg
    25141 atgttgtgag tagtttaaag ctacccgcaa attctttcgt tgtgctggca caggcggtta
    25201 aaccgtcggt aactggataa tccccattgg gggaaagctg gctacacccc tcacatctga
    25261 ggactgataa ccagcaagcc ggaatatatt cacaaactac gaaattttcg tagtttaact
    25321 ttcatccagt acatccccga aaacacgggt atgtacggag ttaccgttac caataattat
    25381 tcgcgaggtg attgttgatg cgtgatattc agcaggttct ggaacgctgg ggagcgtggg
    25441 cggcaaataa tcgcgagaat gtgacctggt catccgttgc cgcaggattt aaggggatta
    25501 ttccggcaaa gataacatca cgctcacagt gctgtgacga tgatgcgatg gtgatatgcg
    25561 ggtgcatagc ccgcctttac cggaacaatc gcgatctgca tgacttgctg gttgattact
    25621 acgtgctggg gaaaacgttt atggcgctgg cacataaaca tgaatgctct gattcactca
    25681 tcggaaagcg gctacagaaa gcggaaggtg ttgttgaagg catgctgatg atgctggatg
    25741 tgaagctgga gatggatcgg tatgttgagc gcgattcagt gacggcataa cgcgcataaa
    25801 aaaaccgcag ccatgaggaa actgcggtgg agaaatacgg cttgccaatc aatcatgaaa
    25861 aacgtaatcg cctgataatt atgccacgag acaaaggacg gtgcaccctg aatggtcata
    25921 atgaccactc agaccatcaa ggcaaccata acgaacatcg ttacagttgg agcttttacc
    25981 ccgttggggg aaaaggcgca tacctgaatc atcattatgg tgatcaaggg tatgtaaagc
    26041 ccaccagcct gattaaaggt taaccggaaa aagccaggta tggagggagt ggttagccac
    26101 gaacaagccg gacaccgcgc cagcaaaaac cacagattcg tgcttttctt cagccagggt
    26161 aagaacgcca cggggattaa tccgtcgtct ggtggaatca gtcggcagca tgacgaacag
    26221 gccgcgcagc cggacaccgc cagcagaagg tattatgatt ttcgtaatac ctttagcaaa
    26281 gcgtgaatca tcattaccgt agggatggtc ataatgacca caccttcccc caccggaaca
    26341 aggaaggcag accaacgaag atttcgtaag tcctgacccg cacaaggtat taaataatca
    26401 ctacaagcca ttgattaaag ttaattataa cgattcgtta gaatcaggct tttcagtctg
    26461 gttaacggtt gaccggaaaa aatctggtag ggtatccgcg atgttgggtt tattcatcgc
    26521 ctgatgttgc ctcaaatccc cctattgttg cctcaaatca gcatgatgtt gcctcaattt
    26581 ttaagacgat attcaattaa atcaatgcgt taattgattt gaggcaactg aggcgactga
    26641 ggcgacacaa tttcgctata tatataaagc gagtgagtga tctgaatggt catgatgacc
    26701 acaccccacc ggaacaagga cagaagaatt acgaaaattt cgtaaaatcc tccccgcatc
    26761 ccccacggtt gcccgttaag tgcaagcgca caggcacgac ctcgttaacc agttaccagc
    26821 gctcacgctc aaatatccac aggaatgggg ataaatcgta ttctggcgca atgttcactg
    26881 tcggggcgag tattcaccgg aaaacgtcat acaggcttac agtcggctta aaattagatg
    26941 gacggatgac agggcgggat ggtgagacct acacaaaaag tgtaggtctc catgacacgg
    27001 tcaggacagt ttgtgtttaa ccagaatatt tagcgcctgt atcggggctt cgtggagttc
    27061 ccgcagacat tcagggtcag taacatgttc ctggtatctt gttttctcaa gtaacaagcg
    27121 gtgcatatgc agcatacggg caagagccgc gcacacctcc tcaaatgcgg tatcccgctt
    27181 accgtcactg tcagagccac aaaacagcgg tacaacctcc acgccgtcgc tgtgggcctt
    27241 tagccaggca ttaaccacgc tgaaatcatc ggtgaagaac cacgcactgt tagcgctgaa
    27301 tcgcattttc caggcttccg gtactggttg cggggagtat gaatatgctg gtggtatctt
    27361 gtcgtgagta tccataggct atcgttccgt cattctgtta ttcaccacag cggtaaaaac
    27421 gccatgaagg accagaatac cgattgcctc gtttgctgta tgaggtgctt accacacatt
    27481 aggtacacag atgaatcagg tggaagtgtg attaccggaa aaaatccctt tcagtttgac
    27541 gaaaaaaaac cgcagtgtga taacactacg gcacgccacc aaagaaacat aacaaggaat
    27601 caacaatgag taacacctgt aagccttcag ggtgattgta acacatgagc aatcttttac
    27661 tagaagctgt ttttcactgg atcacatatc gttatgggga ttcccatatc ggagagcatc
    27721 caccagcttc ataacggata accgaaaaat gataacggtt aacctagaaa aaattccggt
    27781 gaatatttgc cctgacagtt cccatatcgg cggcacacct gaaaaaaccg tgtacacaag
    27841 ggtgtacaca ataaaaatcg ttattacaga aaccccagca atgacaagtc tttgatgcat
    27901 atactcaatg agcagattca acattgaata actcgtaacg ccaattctta cttttcagcc
    27961 ttcacaaatg ccgcccactc aaacagagcg gcatttttct tctccgcaac tttcaattct
    28021 gttttgcgtg cctgttccag attttgcgat gtctttttgc ccagcacact gagaacgtga
    28081 gatactcaca actgtatata aatacagtta cagatttact ttctttgcaa tcgatatcac
    28141 atggagtggg caatgaacga acatcatcaa ccttttgaag aaatacggca ttatggcact
    28201 gaagggcaag aattttggtc agcgagagaa ttggcaccgt tactggatta tcgggactgg
    28261 cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt gcgaagccag caatcaagct
    28321 gcatctgacc atttcgttga aaccaccaaa atggttgtat taggttcagg ggctcaacga
    28381 gaacttgaag atgttcatct ctcccgctat gcctgttacc tggtagtaca aaacggcgac
    28441 cctgcgaaac cggttattgc ggcagggcaa acttattttg ctatccagac ccgacggcag
    28501 gagctggctg acgatgaagc tttcaggcaa cttcgtgaag acgaaaagcg tctttttctg
    28561 agaaatgaat tgaaagaaca taacaaacaa ttagttgagg cagcgcagca agctggggta
    28621 gcgacagcta tcgatttcgc catatttcag aatcatggtt accgggggct gtatggtgga
    28681 ttagatcaga aagctattca tcagcggaag gggctgaaaa agagtcagaa gatcctggat
    28741 catatgggtt caacagaact ggcggctaat ctctttcgag ctacccaaac agaagaaaaa
    28801 ctcaagcggg atggcgtgaa ttcaaaacag caagctaaca ccacacactt tgacgtgggt
    28861 cgcaaggtga gacaaaccat tcaggaactt ggcggaacca tgcctgaaga gttgccgacc
    28921 ccgcagatca gcatcaagca gttggaaaac tgtgtaaaaa ttacagagaa gaaatagcag
    28981 aaactaaaaa ctctttttgt tgattgagac acccgatgcg taagccaaag tccaggtgcg
    29041 gtgaatacaa tagctaaaca ttgcaccttt cttttccacc cctcagtttt aacctatttt
    29101 ttcttatgca ttttctcaga caagaagtca gaagaatgca tctccgctac agaaaatagc
    29161 gatttcacat aactacaagt tatcaattcc ccctcacctt aaaaaatctc aatcgtgaca
    29221 atgcgcacaa atcgctaccc tgcaagacag atttttaggg agagaaccat gctgttacac
    29281 attttgtatt tggttggcat tactgccgaa gccatgacgg gggcgctggc ggccgggcga
    29341 cggcgcatgg atacatttgg cgtaattatt attgcgaccg ccaccgcaat tggcggaggg
    29401 tcagtgcgcg atattctgct gggccactat ccgcttggct gggtcaaaca cccggaatat
    29461 gtgattatcg tcgctaccgc cgcagtactc accactatcg ttgctcctgt aatgccttac
    29521 ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg tggtcttttc tatcattggc
    29581 gcacaggttg cactggatat ggggcacggc ccaattattg ccgttgtcgc ggcggtaacc
    29641 accggcgtgt ttggcggcgt tttgcgcgat atgttctgta aacgcatccc actggtattc
    29701 cagaaagagt tgtatgccgg tgtctcattt gcctccgccg tgctgtacat cgcgctgcaa
    29761 cactatgttt ctaaccatga tgtggtgatt atctccaccc tggtattcgg cttttttgcc
    29821 cgtttactgg cgctacgtct taagctggga ttaccggttt tttactacag ccacgaaggt
    29881 cactaaggtt caaaacctgt gatctgctgg gcagccagcc aactgcccag cttcttgatt
    29941 tgcgcatttt ccttccattc aataacctgt ctggcgcgcc cagatccaat ccccggcagc
    30001 tgctgccaga actgttccgt gctaactaaa agttgcgacc aggaccgttc atcactggca
    30061 ttaagcgccg cccgggttag cggtattccc attgccatca cccagcgagt aaaaggctgc
    30121 ttacgagcct gattaaactg atgccataac tgcgcacttt tacttttcgc gatccccggc
    30181 gtgttctgta attgctctgg cgttaataaa agccaggaaa agatatgctc aaagcgatga
    30241 gtctgatgca gcgcgcgcca accggcctca ccaatgccat ccagccccag aacctgtttt
    30301 gaccccagcc agactaagcg tgaaatgaac tgttcctgac aaacatcaga agcaaagtag
    30361 caggtcaacg agttaaagcg gttttctggt ggtgtcggtt ttgtacgttc tgcaccgcgc
    30421 cagaccacat catcaatgcg aggaataccc tgaccggcaa ggctgacgag gatctgatca
    30481 ccaggtgcaa tatcccactc ctgccagcgc ctgacggaac caatattcac ccgctggact
    30541 tttttatcat ccagcatgac aggtgcgagt gacgcaacca ccgatatttt accgctctta
    30601 cccaccgcaa actgaatcgc cttcacatcg gcaacctgag ctacaggttg atatttccag
    30661 gctaccagcc actctgcctg gcccggtagc caatggcggg attctggctc tttcgccgct
    30721 cgtacaacta cgccatcggt gacgaagggt aatttcgctt tccaccactc attgcgtacg
    30781 cgcgcaactt catcagcatt tttcaccgca cgggtatacg tctgcgttag agtaaaacca
    30841 gcggtagcca gctcttttaa acgatcggac attaactgcg gtccatccgg ccatgcccag
    30901 acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct ggcgcatcat caggccagca
    30961 acttttgcgc gggcatttat tccccccatt tgttgttgga tatgcccctc gcgctggaga
    31021 aatatttccc cctgaagcgt actgttggct aaaggcccgc taacggtttg cggcacagcg
    31081 gaaattaagc taactttctg cgtccagtcc tcgcctttca ggccattacc gcgactgatt
    31141 gctttgttca gtttcccgtc ccgataaacc agggttaccg caacgccatc aacttttggc
    31201 tgcacccaaa gatcgctacg ttctcgcatc cacagactta atgcgttttt atccaccatt
    31261 ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac catttaaagg cggcatcatc
    31321 acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg taagacgggc gcttaactga
    31381 tcgtaaacac cgtcttccac ctcacttttt ccttccttcc agtagtcatc gtcccactgt
    31441 tttatttgct gttgcaggcg ggaaatttct tcctgtgctc tggctggcga ccaggccgga
    31501 cagaccgccc acacagatga ttgccagcac aagatactta ttaatatcgc catccatact
    31561 ttcatcatca cctccgctgt agatagtcag gcagatatac aacgtgatga aagcaaagcc
    31621 gagtggcaaa aacggagtct gcgaggacgc ttcctgagaa tcgtctttat tgcagtgaat
    31681 cactggcaaa tgcggaagca gctacgcaaa acgcaacaac tttgcgcaaa aagtgtgagc
    31741 aagggctacg tcacatggcc gcgccgtgta taataagctc gtatgtaggc tttatttcgc
    31801 taatcacata cgaaagatac tcatggctca aggcacgctt tatattgttt ctgcccccag
    31861 tggcgcgggt aaatccagcc tgattcaggc tttattaaaa acccaaccgt tgtatgacac
    31921 ccaagtttct gtttcacaca ccacacgcca accgcgtcct ggtgaagtcc acggtgaaca
    31981 ttatttcttt gttaatcatg atgaatttaa agaaatgatt agcagagatg cgttcctcga
    32041 acacgcagaa gtttttggta attactatgg cacttcgcgt gaggccattg agcaagtact
    32101 ggcgaccggt gtcgatgttt ttctcgatat cgactggcag ggtgcgcagc aaattcgcca
    32161 gaagatgccg cacgcgcgga gtatctttat tttaccgccg tccaaaattg aactggaccg
    32221 ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt gcaaagcgta tggcgcaagc
    32281 tgttgcagaa atgagccatt acgccgaata tgattatctg attgtgaatg atgacttcga
    32341 taccgcgttg accgatttga agaccattat tcgcgccgaa cgtctgcgca tgagccgcca
    32401 aaagcagcgt catgacgctt taatcagcaa attgttggca gactgaacct gatttcagta
    32461 tcatgcccag tcatttcttc acctgtggag ctttttaagt atggcacgcg taactgttca
    32521 ggacgctgta gagaaaattg gtaaccgttt tgacctggta ctggtcgccg cgcgtcgcgc
    32581 tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg gaagaaaacg ataaaaccac
    32641 tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac aaccagatcc tcgacgttcg
    32701 cgaacgccag gaacagcaag agcaggaagc cgctgaatta caagccgtta ccgctattgc
    32761 tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat ctgtttgaaa gcctgaatca
    32821 gctgattcaa aactacctgc cggaagacca aatcaagcgt ctgcggcagg cgtatctcgt
    32881 tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt gaaccctata tcacgcaccc
    32941 ggtagctgtt gcctgcattc tggccgagat gaaactcgac tatgaaaccc tgatggcagc
    33001 gctgctgcat gacgtgattg aagatactcc cgccacctac caggatatgg aacagctttt
    33061 tggtaaaagc gtcgccgagc tggtagaggg ggtgtcgaaa cttgataaac tcaagttccg
    33121 cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg attatggcga tggtgcagga
    33181 tatccgcgtc attctcatca aacttgccga ccgtacccac aacatgcgca cgctgggctc
    33241 acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact ctcgaaattt atagccctct
    33301 ggcgcaccgt ttaggtatcc accacattaa aaccgaactc gaagagctgg gttttgaggc
    33361 gctgtatccc aatcgttacc gcgtaattaa agaagtggtg aaagccgcgc gcggcaaccg
    33421 taaagagatg atccaaaaaa tcctctctga aatcgaaggg cgtttgcagg aagcgggaat
    33481 accgtgccgc gtcagtggtc gcgagaagca tctttattcg atttactgca aaatggtgct
    33541 caaagagcag cgttttcact cgatcatgga catctacgct ttccgcgtga tcgtcaatga
    33601 ttccgacacc tgttatcgcg tgctcggcca gatgcatagc ctgtataagc cgcgtccggg
    33661 tcgcgtgaaa gactacatcg ctattccaaa agcgaacggc tatcagtcgt tgcacacctc
    33721 gatgattggc ccgcacggcg tgccggttga ggtccaaatc cgtaccgaag atatggatca
    33781 gatggcggag atgggtgttg ccgcgcactg ggcttataaa gagcacggcg aaaccagcac
    33841 gactgcacaa atccgcgccc agcgctggat gcaaagcctg ctggagctgc aacaaagcgc
    33901 cggtagctcg tttgaattta tcgagagcgt taaatccgat ctcttcccgg atgagattta
    33961 cgttttcaca ccggaagggc gcattgtcga actgcctgcc ggtgcaacgc ccgtcgactt
    34021 cgcttatgca gtgcataccg atatcggtca tgcctgcgtg ggcgcacgcg tcgaccgcca
    34081 gccttacccg ctgtcgcagc cgcttactag cggtcaaacc gttgaaatca ttaccgcacc
    34141 gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt agctcgaaag cgcgcgccaa
    34201 aattcgtcag ttgctgaaaa acctcaagcg tgatgattct gtaagcctgg gccgtcgtct
    34261 gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa atcccgcagg aaaatattca
    34321 gcgcgagctg gatcgtatga agctggcaac gcttgacgat ctgctggcag aaatcggact
    34381 tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa catggggacg cctccattcc
    34441 accggcaacc caaagtcacg gacatctgcc cattaaaggt gccgatggcg tgctgatcac
    34501 ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt atcgcccacg tcagccccgg
    34561 taaaggtctg gtgatccacc atgaatcctg ccgtaacatc cgtggctacc agaaagagcc
    34621 agagaagttt atggctgtgg aatgggataa agagacggcg caggagttca tcaccgaaat
    34681 caaggtggag atgttcaatc atcagggcgc gctggcaaac ctgacggcgg caattaacac
    34741 cacgacttcg aatattcaaa gtttgaatac ggaagagaaa gatggtcgcg tctacagcgc
    34801 ctttattcgt ctgaccgctc gtgaccgtgt gcatctggcg aatatcatgc gcaaaatccg
    34861 cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat taatgtttta tgaacccaac
    34921 acgttatgca cgcatctgcg aaatgctcgc caggcggcag cctgatctga ccgtctgcat
    34981 ggagcaggtc cacaaacctc ataacgtttc tgcgattatt cgtaccgcag atgccgttgg
    35041 cgtacatgaa gttcacgccg tctggcctgg tagccgcatg cgtaccatgg cctctgcggc
    35101 ggcgggtagc aacagttggg tgcaggtgaa aacgcaccgc accattggcg atgccgtcgc
    35161 tcatctcaaa ggccagggca tgcaaattct ggcaacccat ctttctgata acgctgtcga
    35221 ttttcgcgaa attgattaca ctcgcccgac ctgcattttg atgggtcagg agaaaacggg
    35281 catcactgag gaagcgttgg ccctggcaga tcaggacatc atcattccga tgatcggcat
    35341 ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt ctttacgaag cccagcgtca
    35401 gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg ttgccggaag cagagcaaca
    35461 acgcctgttg tttgaaggcg gctatccggt gctggcgaaa gtcgcaaaac gcaaaggcct
    35521 gccttacccc cacgtcaatc agcaaggcga gatcgaagct gatgccgact ggtgggctac
    35581 catgcaggct gcagggtaag tgccatgaaa ggtcgcctgt tagatgctgt cccgctcagt
    35641 tccctaacgg gcgttggcgc agcgcttagt aacaagctgg cgaaaatcaa cctgcagact
    35701 gtgcaggatc tactcttaca ccttcccctg cgctacgaag atcgcaccca tctctacccc
    35761 atcggagaac tactaccggg cgtttatgcc acggtggaag gcgaagtgct gaactgcaat
    35821 atctccttcg gcggtcggcg gatgatgacc tgccagatca gcgacggttc cggcattctc
    35881 accatgcgct ttttcaactt caacgcggca atgaaaaata gcctggcgac gggccgccgc
    35941 gtgttggcct atggagaagc aaagcgcggt aaatatggtg cggagatgat ccacccggaa
    36001 taccgcgtgc agggcgatct cagtacgcca gagttgcagg aaacgctcac gccggtttat
    36061 ccaacaaccg aaggcgtaaa gcaggccacg ctgcgtaaat taaccgacca ggcgctggat
    36121 ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg aactgtcaca aggaatgatg
    36181 acgctaccgg aagcgttgcg cactttgcac cgcccgccgc cgacgctgca acttagcgat
    36241 ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc tggaagaact gctggcgcat
    36301 aacctcagca tgttagcctt acgcgccgga gcgcaacgtt tccatgccca gccgctgagc
    36361 gccaatgaca cgctgaaaaa taaacttctt gccgccttac cgttcaagcc aacgggcgct
    36421 caggctcgcg tggtggcgga gatcgagcgc gatatggcgc tgaatgtgcc gatgatgcgc
    36481 ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg ctgccctcgc cgcgctgcgt
    36541 gcgattgccc acggcaaaca ggtggcattg atggcaccaa ccgaattact cgccgaacag
    36601 cacgccaata acttccgcaa ctggtttgca ccgctcggta tcgaagtggg ctggctcgcc
    36661 ggtaagcaga aaggtaaagc gcgactgtct cagcaggaag ccatcgccag cggtcaggtg
    36721 cagatgattg ttggtacgca cgccatcttc caggaacagg tgcagtttaa cggtctggcg
    36781 ctggtgatta tcgacgaaca gcatcgtttt ggcgtgcatc agcgtctggc attgtgggag
    36841 aaaggccagc agcaaggctt ccatccgcat cagttgatca tgaccgccac gccgatcccc
    36901 cgcacgctgg cgatgactgc gtatgccgat ctcgacacct cggtcattga tgaactgccg
    36961 ccaggccgca cgccagtgac tacggtcgct attcctgata ctcgccgtac cgacatcatt
    37021 gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg cgtactgggt ttgtacgttg
    37081 attgaagagt cggaattgct ggaagcgcag gcggcggaag ctacctggga agagttgaaa
    37141 ctggcgctac cagagttgaa tgttggcctg gtacacgggc ggatgaaacc tgccgagaaa
    37201 caggcggtga tggcgtcgtt taaacaaggt gagctacacc tgctggttgc cacaaccgtt
    37261 attgaagtcg gcgttgatgt gcctaacgcc agtctgatga ttatcgaaaa cccggagcgt
    37321 ctgggtctgg cgcagttgca ccagctacgt gggcgtgtag gtcgtggcgc ggtggcttct
    37381 cactgcgtgc tgctctataa aacgccgctt tctaaaacgg cgcaaattcg cctgcaagtg
    37441 ctgcgcgaca gcaacgacgg gtttgtgatt gcacaaaaag atctggagat tcgcggccct
    37501 ggcgaattgt taggcactcg tcagacgggt aatgctgaat ttaaagtggc ggatttactg
    37561 cgcgatcagg cgatgatccc ggaagttcag cgcctggcac gccatattca cgaacgttac
    37621 ccacaacagg caaaagccct gatagaacgc tggatgccgg agacggaacg ttactcgaat
    37681 gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta ccctaccttc ctgccggatg
    37741 tgcttcgtcc tacaaaaact tataaattca ataaattgtg aatcaatgcg cagccttgat
    37801 aagcgaagcg tatcaggcat ttgtctttgt tgcggcggca cttctaccgc cgcttcatcg
    37861 gttaacccgc aaaaatcggc aacatcaaat acagcttaat caccagcgca ttgacgatat
    37921 cgataaagaa cgcaccgacc atcggcacca ccagaaacgc catgtgcgac gggccaaaac
    37981 gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt tgcaccgagg ccaaaaccac
    38041 agtggcccgc agccagcact gccgcatcgt agtttttgcc catcatgcgc caggtaacga
    38101 agatggcata caacgccatg aagatggtct gtaccaccag aatcgccagc atcggcagcg
    38161 ccagcgaagc cagctcccac agtttcagcc ccatcagcgc catcgccagg aacaacgaca
    38221 ggcttacgtt acccagcacg gataccgcac gctcaaagac gcggtaaaag cccatcattg
    38281 acagaccgtt gctcagaatc acgccaacaa acagtacaca gacgaaggtc ggcagttcaa
    38341 aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac cgtcaggcag atagcaatca
    38401 gcgcgatagt ttcaatcagc accagcgagg tgatcatgcg gcccacatcc ggcttttcaa
    38461 acgccgtcgg gacttcctgg tcatccggaa taccgttcgg cgtggtggag tgtttcacca
    38521 gatagcgcgc caccggaccg ccaatcaagc cgcccagcac cagaccgaac gttgcacagg
    38581 ccatcgccac ttctgtcgca ttggtgaagc cataacgttc aatgaacaat ttactccatg
    38641 cagctcccgt accgtgaccg ccggaaagcg taatagaacc ggccaacagc cccatcaacg
    38701 gatcaagccc taacaggcta gccatcccaa tgccaatggc attttgcatc accaacagac
    38761 caacaaccac aatcaggaag atgccaacca cacgcccacc ggcacgcaaa ctggcaatgt
    38821 tggcgttcag gccaatggtg gcgaagaatg ccagcattaa cggatcgcgc agggacatat
    38881 caaagttgac ttcccagccc atgctttttt tcagtaccag tagcgccagc gccaccaaca
    38941 aaccacccgc aacaggttcc ggtatggtgt atttcttcaa aaaggagacg gaatggacca
    39001 acttacgccc gagcagcaac gtcagcgttg cggcaacaag cgttgctaaa gtatcgagat
    39061 gaaacatagt tactcctttg tatccgcatc actctttcat actcgtcata cttcatcgcg
    39121 cagtggcaac ttatgaacta tgacgggtat cgagttatgc tttcctgggt caaattctca
    39181 gctgttaatg aacaaatggc atggatttta agcagaaaaa taccaaaagt tatataaaaa
    39241 agtgcattta tctcatttaa atatttagat gaggatgtgg caaacgtttg cttttctctt
    39301 tcggtcagct aaaatgcccg ctttgctacc acgggattgt tttcgatgtc tgtttccacc
    39361 ctcgagtcag aaaatgcgca accggttgcg cagactcaaa acagcgaact gatttaccgt
    39421 cttgaagatc gtccgccgct tcctcaaacc ctgtttgccg cctgtcagca tttgctggcg
    39481 atgttcgttg cggtgatcac gccagcgcta ttaatctgcc aggcgctggg tttaccggca
    39541 caagacacgc aacacattat tagtatgtcg ctgtttgcct ccggtgtggc atcgattatt
    39601 caaattaagg cctggggtcc ggttggctcc gggctgttgt ctattcaggg caccagcttc
    39661 aactttgttg ccccgctgat tatgggcggt accgcgctga aaaccggtgg tgctgatgtt
    39721 cctaccatga tggcggcttt gttcggcacg ttgatgctgg caagttgcac cgagatggtg
    39781 atctcccgcg ttctgcatct ggcgcgccgc attattacgc cgctggtttc tggcgttgtg
    39841 gtgatgatta tcggcctgtc gctaattcag gtggggctga cctcaattgg cggcggttac
    39901 gcagccatga gcgataacac cttcggcgca ccgaaaaatc tgctgctggc aggcgtggtc
    39961 ttagccttaa ttatcctgct taaccgtcaa cgtaaccctt acttacgcgt ggcctcactg
    40021 gtgattgcga tggcggccgg atatgcgctg gcgtggttta tgggcatgtt gccagaaagc
    40081 aacgaaccga tgacgcaaga actgattatg gtgccaacgc cgctctatta cggtcttggc
    40141 attgaatgga gtctgctgct gccgctgatg ctggtcttta tgatcacttc gctggaaacc
    40201 attggcgata tcacggcgac ctctgacgtt tccgaacagc cagtgtccgg tccgctgtac
    40261 atgaaacgcc tgaaaggcgg cgtgctggca aacggcctga actcgtttgt ttcggcggtg
    40321 tttaacacct tcccgaactc ctgcttcggg cagaacaacg gagtgatcca gttgaccggt
    40381 gttgccagcc gctatgtcgg ttttgtcgtc gcgctgatgt tgatcgtgct gggtctgttc
    40441 ccggcagtga gcggttttgt acaacacatt ccagaaccgg ttctgggcgg cgcaacgctt
    40501 gtaatgtttg gcaccatcgc cgcctccggt gtgcgtatcg tttctcgtga gccgctgaac
    40561 cgtcgggcga ttctgattat cgccctgtcg ctggcggttg gtctgggcgt gtctcagcag
    40621 ccgctgattt tgcagtttgc ccctgaatgg ctgaaaaacc tgctctcctc cgggatcgcc
    40681 gcgggcggta ttactgccat cgtgctgaat ctgattttcc caccagaaaa acaataattc
    40741 actggcggcg gtaagataat taccgccgca gtctcacctc tttcaccatt cccgccttga
    40801 ggattgcgcg taaatcgtgc ataactccct tatgtgcatt tcacgggatg gaagaccatg
    40861 aaatttattg ggaagctgct tctctacatt ctcatcgctc tgttagtggt gatcgctggc
    40921 ctctattttc ttctgcaaac ccgctgggga gcagaacata tcagcgcatg ggtttccgag
    40981 aatagcgact atcatctggc cttcggggcg atggatcacc gtttttccgc gccatctcat
    41041 attgtgctgg agaacgtcac gtttggtcgt gatggccagc ccgcgaccct ggtggccaaa
    41101 agtgtcgaca ttgcgctaag tagtcggcaa ctgaccgaac cacgccatgt cgataccatc
    41161 ctgctggaaa acggaacgct gaatctcacc gaccagaccg cgccgctacc gttcaaagcc
    41221 gatcgtctgc aactgcgtga tatggcgttt aatagcccga atagcgaatg gaaactgagc
    41281 gcgcagcggg taaatggcgg cgtggttccg tggtcaccag aagccggtaa agtgctgggt
    41341 acgaaggcgc agattcagtt tagtgccgga tcgctttcgc tcaatgatgt tcctgccacc
    41401 aatgtactga ttgaaggcag tattgataac gatcgcgtta cgctgactaa cctgggtgcc
    41461 gacatcgcgc gcgggacatt aaccggaaac gcgcagcgta acgccgacgg cagctggcaa
    41521 gtggaaaatc tgcgcatggc ggatatccgt ctacaaagcg aaaaatcgct aaccgacttc
    41581 tttgcaccat tacgctctgt cccgtcgttg cagattggtc gcctggaagt gatcgatgct
    41641 cgtctgcaag gtccggactg ggcggtgacc gacctcgatc tcagcttgcg caacatgacc
    41701 ttcagtaaag atgactggca gacacaagaa ggcaaactgt cgatgaacgc tagcgagttc
    41761 atttatggtt cgctgcattt atttgacccg attataaacg cggaattttc cccgcagggc
    41821 gtagcgctgc gccagttcac cagccgctgg gaagggggta tggtcagaac gtcagggaac
    41881 tggctgcgtg acgggaaaac gttactcctt gatgatgcgg caattgccgg gctggaatat
    41941 accttgccga aaaactggca acagttgtgg atggaaacga caccgggttg gttaaacagc
    42001 ctgcaactga agagatttag cgccagccgc aatctgatca ttgatatcga ccctgacttc
    42061 ccgtggcagc tcaccgcgct cgatggttac ggtgccaacc tgacgctggt taccgatcat
    42121 aaatggggcg tctggagtgg ttcggcgaat ctgaatgccg ccgccgcgac gtttaatcgt
    42181 gttgatgtcc gtcgtccatc gctggcgcta accgccaaca gcagcacggt gaatatcagc
    42241 gaactgagtg catttactga aaaaggcatt ctggaagcca ccgccagtgt ttcacaaacg
    42301 ccacaacgtc agacccatat cagcctgaat ggacgcggtg tgccggtgaa tattttgcaa
    42361 cattggggat ggcctgaatt accgttgact ggcgacggca atattcagct taccgccagt
    42421 ggcgatattc aggccaatgt cccgctgaaa cctacggtta gcgggcaatt gcatgccgtg
    42481 aacgccgcaa agcagcaagt gactcaaacc atgaatgcgg gcgtagtttc cagtggtgaa
    42541 gttacgtcga cggagccggc gcagtaaaaa taaggacgct agcggatatc agcgctagcg
    42601 ttccccgtta ttagcgcaaa attccatact cactgcacaa taaccatttc accggttcat
    42661 cttcttctat cgcaggaaaa cgtcctcccg gctcaagaaa atagttatcg tgttggtcat
    42721 catttaccat cgaaatttcc cagccgagaa caaacgcttt ttccgcccag aagcgatgat
    42781 acagacgcgg cggcaggcaa atgccctgcc ccggctttaa caccagcgtt tcaccaggtt
    42841 tcatggtacg ccgctgacca tcaacactca cggtaatgac tcgttgatca tcacagagac
    42901 tggcttcgtt ggcccaggcc aactgcatgc ataagtctcc accgccccga ttgaggatat
    42961 cttccatttt gtgggtgtga aaatgccagg gagtctgctg atcttgttgg atctgcaaca
    43021 ttttctcagc atacggtttt ggatatgccg ccgaccccag cgcgccgttg cgcatcgtta
    43081 gcagtgttag tcccgtttgg ttgaaatcac cacgaccaaa atcggtgata tcccaaccga
    43141 gctgtaagtc gaccacttct tgccagttat cccgttcccg ctgcagccag tcatccaccg
    43201 taaaataggc gaactctggc aaacaaacgc ggaaggtctg cgcaatggca tgtgctttat
    43261 caataacata attaatttgt gaacgtttca ttggcttctc cgttgcgcat agcaagccag
    43321 gcagcgccac gcattccgct actgtcgccg tgctgtgctt ttacaatggg agtggtgaat
    43381 tgatcggtga aaaccagtgg agcgacttct gcgttcagat cggtgataag cagttccaca
    43441 ttcgaaagcc cgccgccgat cacaatcacg ccagggtcaa caacattaac gatggttgcg
    43501 agcgtgcggg caagctgttg gcgaaaacgt gccacctgct gcatggcaca agcatcacca
    43561 cgttgtgcca gggtgacaat cgccgcagga gtgagcaagt taccagtcat ctgttgatag
    43621 cgttcactaa aaccgctgcc ggaaacaaac gactccacgc agttgtgttt gccgcaatag
    43681 caactgactg atggaccatc ttcctgctcc gtatagcctg gcaacgtgat atgaccgcat
    43741 tcggcagcat ttccccaggc ccctataaac ggtcgttgct tgatggcaat gccaccgccg
    43801 cagcccgtac caagcgtaat accaaatacc acgtcataat cttgcccggc accgtcgcaa
    43861 gcttctgata gcgcgaaaca gttaccatca ttggctatca ccaccggttg cccaagcaat
    43921 tgctgcaaat cagcctgtaa cgcatgaccg ttaatcacct gaatattcgc gtttttaatc
    43981 aggccagtga gtggcgagat actgccgggt aaggcgatcc ctgtcagcat cggtcgttga
    44041 acgtcccgcc taatctgctc gataagtgcc acaacgcatg agacaaattg ttgatatgtc
    44101 gacttttgcg tcgggcagcg gtaacggcga atctcccagc catgcgcatc catgacgacg
    44161 gcggcgattt tggtcccacc aatatccaac cccagatagt gcatatctcg ttccttgttg
    44221 cgtaaaaggc gggtcacccc gcccttctgg cttagtacag tgatgcttta ccgagcgaac
    44281 cgaacagttt cattttgtgg cgaatgactt cccgcgccgc gttaatcgca tccggataga
    44341 tgacgttcgg gtcccaccag ctctctttag cgagaatttc acgcactttc tggaaatagg
    44401 cgtatttcat atcgctggag atattgattt tgccgacgcc gagcgtaact gattcagcaa
    44461 tctccgcgtc cgggtttgcc gaaccaccgt gtagtaccag cggaatatca atacgtccgg
    44521 caatgtcgcg caaaatgttc atttgcagct tcggttgcat cccttttgga tagatgccat
    44581 gcgctgtacc aatcgccaca gccagtgtgt cggcacctgt acgagcaata aaatcggcgg
    44641 cctgcgccgg atctgtgtag gtcacttgtg aaacaccacc ttctaccgac gttcctgttt
    44701 gtccgatggt tcccagttca ccctcaaccg acacgccaac cgcgtgagcg aggcgaacca
    44761 cttctgcggt cagcgccacg ttttcttcat acggcagcag cgaaccgtcg atcattactg
    44821 aggtaaatcc gcagcgaatc gcacgcaaaa catgctctac agaggcacca tgatcgaggt
    44881 gcagtacaaa cgggactgga ctacgctggg taatttcgcg aacgtgagcg aaaaaggcat
    44941 cgcccacaaa atcatgttcg ctgggatgga tggcaataat cgccggagta tttgtcgctt
    45001 ctgcttcatc cacgactgcg cgaaggaaac agttatccgc cacattaaat gcgccaatgg
    45061 caaagccgtg ctggcgggta ggttgcagta attctttcat cgaaactaac atgttaaatc
    45121 tccagtttca gcgtgtagtc cgccctgccg gtgcgttatt gcactggcat aaaggccatg
    45181 acagaaaatg aatcaggctt tgccgataga gccgctcgcc tgtaaatacg gtgtaatcgc
    45241 ttcttcgaag gccagttcca tagctttcag cgcgtccacc agttcaatgg atatatcgtg
    45301 acagagggta tgcaggagcg cggatttccc ccgttcaaaa atggccgccc ccacgttgat
    45361 ttttgctacc cccagcgtcg tacagagctg gagctgatcg aacggcgtac cgctaccgcc
    45421 atgtaatgcc agaggcacgc tcgagcagtg gaaaatctcc gccagacgct gaaaatcaat
    45481 agcaggtaca acaccaggcg cgtacatgcc gtgcgccgta cccacagaga cagccagtag
    45541 atcaatgcct gtctgcgcga taaacggttc cacatcagtg ggttgcgtca tcatgtctgc
    45601 attggcggcc tggtcgtaac gcggcgcatc tgccagatgg cccagttcgc cttccacgca
    45661 gagctgcgcg ctggcggcga gttccaccac cgccctggtc tggcgcacgt tttcctccag
    45721 gggaaaagca gaagcatcaa tcattaatcc gctgaaacca gcacgaaacg cctgacgaat
    45781 acagtccgga tttttgccat gatcaagcgc cagagtgacc ggtactgagg cagattccgc
    45841 gagcgtttta accactgccg ccgcgacggg tgcaggaaaa tgtgccagat gcccctgata
    45901 gagattgaga atgatcggag cgcgattctt ctccgccacg cggatagctg cgtaagcact
    45961 ttccagattc atacagttga tcgccagcaa ggcgtaatgt tcatgccagg ctttcatcac
    46021 catacttttc atatcggcaa acatagggcc tccggttatt cgagggtgaa agtgatctct
    46081 tcatctttca gcttttgatc gctcagttcg tcaaactctt cgtcgcgttc cgttaccggt
    46141 ttcttccaga gtgaaagcat cacgccacaa atcaccgtgc ctatacccag cgccaggcaa
    46201 aacagcagag gatgggtgaa cagaggcaca acaaacatgc cgccatgcgg aaccggactc
    46261 tccacgcccc acaccatgat taagccgccc gcgaccgccg atgccaccac acaagatccc
    46321 actactcgca gcaggtcacg cgcggcgatc ggaatgactc cttcagtgat catgcagatc
    46381 cccatcggaa acgcggcttt cagcgcctcg cgctccgcac gggtgtattt atggcgtgtc
    46441 agtaagaacg aaagtgtgat gccaaacgga gggatgattg agccgacaaa tttcacggct
    46501 tctggtccgt agacgccact gaccagcaag ccatcggaaa acagcgacat ggttttgttg
    46561 accggaccac cgaaatcgaa ggttgccatc gcgccaagta tggcccccat caggaagcgc
    46621 gatccgccct gcatcgactc gagcaaatga atcagtgctt cttgtaacca ggcgatgggt
    46681 ttaccaatca gcgtcatcat taacagccca ccgataatgg tgcttaatac cggcaacacc
    46741 atgattggca tgagtccttg catcgactgc ggcaggcgga tatagcgttt aagtagcaaa
    46801 atggtgtagc ccaccaggaa accacccaac ataccgccaa taaatccggt atggatttgc
    46861 ccacagagaa agccgacaat cagacccggg gcaaatccgg gacgatcggc aatagaatag
    46921 gcaattgccg cgctaatcag cggcacaatc agccccattc cccagccgcc aatctgatta
    46981 agcatccacg ggattgttcc tgttttttca ccaacatctg taccgccaag aacttgcccc
    47041 aacgcaatac atatcccggc ggccacaata aggggtatca tccacgagat gccggttaat
    47101 aaatgttttt taatttcctg tgaaaccgga attttattgt ctttcatatt aagatccctc
    47161 cagtataagg atcatttgtt tcccagtgat ttttcgacct tttcaagaaa ttgaacgggc
    47221 gatttaatga cgatctctgt ctttactcgc acaatacgtt tattggtgaa gcgttcttct
    47281 ccttttattt tcacatctac agcaagaatc acgacgtcag cagcagagat atctgccgcc
    47341 tgtaattcat tttctgtacc aatggttccc tgggtttcaa ctttaatggt atggcccaga
    47401 gcttttgctc ctttgattaa cttttcacga gcaatatagg tatgagcaat acccgccgta
    47461 caggcggcaa cacagacaat attcatgaca tactccctgt tatttagcag acatcggatt
    47521 gtgcatactg actaaagaga tcaatgattt tttgcggact cgtctcaact aacaattgtt
    47581 ctatgatgtc atcatccgcc agcgcgcccg cgacctggga aagtaaacgg atatgggtgg
    47641 tattttgatc ttcaagacgt accgcaaata aaataataca gcgcacgcta ctaccatcca
    47701 gcgtttccca gggaatatcc tggcgggtac ggccaattgc cagcgtagta ttaattaccg
    47761 cagaagattt accatgcggg atcgcaatat gattttcaaa accagtcgag ccttccgctt
    47821 cgcgctgcca gacatcttta ataaaggttt cccgacaatc gatcgcacca tcttttttga
    47881 gaagattagt taattcttca atcgcctccg ctttattcgt cgcttgcata tctaaaataa
    47941 cgcgattcgt attcaggatt tttgttatgt ccattcgtca tacctcataa aattctgaca
    48001 ttaaatttca acgacccgag acgtcgcatg atgtaatttt tccgtaatca ccatttcagt
    48061 gacccgctta atatcggcat cattaaaaaa agcggtaaca taagcgaccg gcatagtgac
    48121 tgtaggcaaa ttaatggtcg aaataatcag ttcgatattg tcaggcaaaa cctgtgataa
    48181 attcgctgct gaaataacat caacaatcgt ccattcagga aatgctcgca gaatacggct
    48241 tttcagtaga tgcgaagttc cgattccggt tgaacagacc agtaatacac gtttacgggc
    48301 aatttgccgc tccatcgcag cctgaaaatg aacggtcagg taacccactt catcctcgct
    48361 cactgcattc tcaccccagg ttttaaatac ctgattcacc acatattgcg tcaaccgcca
    48421 gacatccgct aattctgctt taatatcttc cagcaatgga ttacggatat gaatacgata
    48481 atttagtcgg ttaatcagcg gtttaatatg caccaataag ccatcatata atgcgctgtc
    48541 ttcactaaaa tcgcagtcca cgatgtcact gaataccgta attaaacgcc aggtaataag
    48601 acgcgcttca ttgctggcct gcatatgtga aataatgcta acatcttttt gtccatcaat
    48661 cgccacacct gatgaaatga tatattgata aataaaccag acttcatcat cgggcaatgt
    48721 atgtgcaatt cgttgttcta tcttatgaat catctgactg gcaacattaa agatattttc
    48781 atcatattgc ctggtttgat cttcttctct tgataacgca ttcccgtgcg tgttgcgata
    48841 catcataata aggatgtgag taaaaatgtt aacgtaataa ggttctccca aagaccaact
    48901 taattcattt tccatatcca gtaacaatga ctggacaaat aacacctctt cttctccaaa
    48961 ataatggact aatgctttat aacttccggg atccagacgt gaatacacga caccttgcgg
    49021 ctcattatgg ttaataatgc cgttaatcag taatgccatt gcctgtcgga cttgcccttc
    49081 actaccttca atatgcgtac cacttgggct gcggatcaac gataacccca acggcgcaag
    49141 ccaggactca attactctca ggtcattaac aatagaggct ccgctgataa agtagcgttc
    49201 tgacaattta ctgatcgacg tttcattcgg tgtttcactt aataactgcg acgctatttt
    49261 aacgcgcctg gcattattca ttacaccatc aatctcttca cattctgctc ccaacaaatg
    49321 atcaagatgc agcaaatcag caatattctc cgcgcttaac atcacacccg cgccagcacg
    49381 cttctcaaca tttattctcc attgccccag ccattgctca agtaaacgta aatcacgttg
    49441 aatggttttt tccgaaacat ttaaatagcc ggcaatctta acaatagtcg tatattccct
    49501 tcgaggtaaa aggaatcgta ataatctgtt ttgtcgcgag gtaatcattt gcatttttta
    49561 cccttatcac agattaagaa gccccatgag gaagagcttc agttgccatc tctgagggta
    49621 aatataaagc agaataattt cacaacaata ctaaaatctg tccgtaatcc ggacagtgat
    49681 aaaacaaagc aaggcggtta agggatattt ttcagcacag atcacatttt cgcgatgaat
    49741 aatcgtgatc atgctcgcaa atacgatgga agaaatattt attaccagat gtgagattat
    49801 ttaccggata cgacacgaaa ccattcgtat ccggcattct tcaatagatt acaacgtaat
    49861 tgtcagcgca ttcccttgag gcgtcaccac cagcccctgc tcactttcag cctgcgaacc
    49921 gccttgcaga ccatttactt tcacaatatt gcgcaagcac agtgtccagt tcctcacctc
    49981 gcccgcacca gtcacagtaa tcgtgttacc agtacgtgtt gcttttagag taaagatcac
    50041 cgaaccgtca gcagcgggca cttcacagac ggcttcatgc ccgtcttgca gattaaagag
    50101 ctggaatgcc gtgccttcgt gccacgcgta atcgggacgt tgatcgttgt tgcccagcgc
    50161 cagtagggtg ttatcacgca cataaatggg cagactcagg aagctgtgct gctgtttatg
    50221 ccagcgacta ccgtcgagct catcgttgtg ccacaggtgt gtccagcgac cttctggcag
    50281 gtagaactgc acatcgcccg cttcagtgaa caccggcgca accatcacgt tgtcgcctaa
    50341 catgtattga cggtcaaggt aatcacaagc cggatcgtcc gggaactcca tcatcatggc
    50401 ccgcatcatc ggcgtaccct gcgcgttcgc acgagcagct tcacgataca gatacggcat
    50461 catgcggcat ttcagttgcg tgaagaagcg caccacatca caggactcat catcgtaggc
    50521 ccacggcaca cgataagatt tgctgccatg taaacgacta tggctggaga gcaaaccaaa
    50581 cgcgcaccag cgtttgtaaa cgtgcgccgg agcggtattc tcaaagccgc cgatatcgtg
    50641 gctccagaag ccaaaacctg aaaggccaat agacaaacca ccgcgcagac tttccgccat
    50701 tgattcgtag ttagcgtaac agtcaccccc ccaatgtacc gggaatttct gcgcaccgac
    50761 ggaggccgag cgggcaaaca agacggcttc ttcctctccg acggtttcct tgagcacgtt
    50821 ccacaccagt tcgttgtaga tgtacgcata atggttatgc attttctgcg aatcggaacc
    50881 gtcaaaccat tgaacatcgg taggaatgcg ttcgccaaag tcggtcttaa agcaatcaac
    50941 gcccatcgcg accagacctt tcagtttgtc ggcgtaccat ttgcaggcat ccggattggt
    51001 aaagtcataa atcgccagac ctggctgcca tttatcccac tgccacagcg aaccgtccgg
    51061 gcgtttgagt aaataacctt tctcttgtaa ctctttaaag acgggggatt tttgcccaat
    51121 ataggggtta atccagacgc agattttcag ccctttcgct ttcagacggc ggatcatccc
    51181 ttccgggtcc ggaaaagtca gcgggtccca ctcaaaatcg caccactgga aggctttcat
    51241 ccagaaacag tcaaagtgga aaacatgcag cggcagattg cgttccgcca taccatcaat
    51301 aaagctattt accgtcgctt cgtcgtagtt ggtggtaaat gaagtggtta gccacagacc
    51361 gaaggaccac gcgggcggca gcgccggacg accagtaaag cgggtataac gatcaagtac
    51421 cgctttcggc gtcgggccgt cgataacaaa gtattcgaga tattcactct caacgctgaa
    51481 ctgtactttg gagactttct ccgatcccac ttcaaaagag acgcattggg gatgattgac
    51541 cagtacccca taaccacggt tagtcatata aaacgggata tttttatacg cctgttcagt
    51601 acttgtgccg ccgtcccggt tccaggtctc taccgtctga ccattgcgca ccagggcagt
    51661 aaagcgctct cccaggccgt aaactgtttc gccaacgcca agatcaagcc gttcaaacat
    51721 ataatttcgt tgattattcg tgtcctgcac gtagccatta tttttcacct gactaccggt
    51781 aatacgttcg ccgttgcgca gaaaatccag tgaccagaac tcacctttgc tgacacgcgc
    51841 gcttaagttg ccacttttaa actcagcata acgttctgtg ttttcgattg tgaccttcac
    51901 gtcctgcaaa atattgagcg gataatgagg gccgttattc agcgccccct gaaaatgctc
    51961 aatccgcacc ccgacaatac cttcctgtgg ggagaaaaag cgcagcgtaa ataaaggcgt
    52021 gtcaagctgc caggtacgtt cacgtacatc acggggggca gcatagacca ccatttcatt
    52081 accctgctgt tcaacctcga atacctgaag cgggtgaatc aaattgaggc caggttgaat
    52141 caaccagttt ccatcgctaa ttttcattgc cgttccttag ttctgcactt cttgagaggt
    52201 gaattgttgc tggcaacggc gtttaccctg agccagctgt tccataacgg ttttcagatt
    52261 gtgcgtcgtg agtgagtagt agcgtttagc gacaatcgcg ctcagcaaat aacagatcgc
    52321 cggaacaatc gtgaatagcg caataatgat gctaatcgtg gcgctgttct gcgctttttc
    52381 tgccgcatca tatccgccat aagccagcat ccagccgata agagcgccgc cgaaggccaa
    52441 ccccagtttg agcacaaaca gcgtgccagc aaaactgatc ccggtcaggc gtttaccatt
    52501 gcaccactcg ccgtagtcga cggtatcgga catcattacc cactggatag gtgtcaccag
    52561 ttgatgcaac acaccaatca cgaagatgaa gacaaacata gtgatgctgg cctgcatggg
    52621 aacaaagaac atcgcgatgc taatcactgc cagcagggcg ttcgtccacc agaagatagt
    52681 gactttacat ttccagtcgg tcagcggttt tgccagtgcg gaaccaatca agttacccac
    52741 gcaataagtg gtgagaaaag cgacaaacac ttccggcgta cccaaaatcc atgtgacgta
    52801 atacatcatc gctccaccgc gtacgcacac agccaggata ttgaaaatgg ttagtaaacc
    52861 gacaatccgc cactggtcgt tttgccagat atcacgtaaa tcttcccgca tagacgtcgt
    52921 tgtaggtggt gcttcaacgc gttctttagt ggtgaagaaa cagaatgcca gcatcatgaa
    52981 tgccaccacg gaaaggaccg cgataccgcc ctggaaaccg agtggtttat tatcaccacc
    53041 aattaaatta accaatggca tcatcagaac cgtagaaagc atgcctcccg cggtcgccag
    53101 cacaaaacgc caggattgca gcgagatacg ctgcgtcggg tcattggtga ttacaccacc
    53161 caatgcgcaa taagggatat taacgacggt ataaagtaag gtaagtaggg tgtaagtaat
    53221 tgctgcataa atcatttttc cgttcatact gagatctggc gtgctatagg ccagtacaca
    53281 gacgatcccg aatggcagtg cgccaaacag tacccacgga cgaaatttac cccagcgaga
    53341 gcgcgttcga tcggccaaca accccatgca aggatcggaa atcgcatcca gtgcacgagc
    53401 gaccaaaaac atagttccga caaatccggc aggaatgcca aaaatatcgg tataaaagaa
    53461 catcatatat aacattacgt tatcgaaaat aatgtggctg gcggcgtctc ccatgccata
    53521 accaattttc tctttaacgg acaacacttc actcttcatc tgcttttcct tgatgctata
    53581 ggggtgctga gaccgataac gcattttgta aaccatccct gtgagagcga atattccgat
    53641 atctggttat taatttatga ttcttgtttt atgtgatcgt ggtagcgtta attccgctca
    53701 tatatcattg taaaatatgg gttttatatg aactataatg ctttcgtgat aatactctgc
    53761 gtgtattagg cggaaaaaac tgatctgggg gatgtagaaa ctcaaggaag tagctataat
    53821 gcgccccgcc tccatgtagc aatcgaggcg cggaagatcg tcgtctccgg tgaggcggct
    53881 ggacttcaaa tccagttggg gccgccagcg gtcccgggca ggttcgactc ctgtgatctt
    53941 ccgccagttt acctcttctg aactctccca atgtcaataa atctcttcta atcccttgta
    54001 tatcagtatt tatttgtatc ctgaggtcaa cctacttcac cccgtatcaa gtttcttttg
    54061 ggggtacttt agggggtgtt atttcctatt gaaatacaag cacccccata ataggaaaaa
    54121 tgtgatacat aacaacggct tttaacaaca tctttgttat acaaggggtg tattgggggt
    54181 atcaggttag ttactggcag aaatccccct caggaacatg aattcatcaa aagccggagg
    54241 atgtcatggc attaaccgat atcaaagtga aaaccgcaaa acccaaagac aaaccctata
    54301 agcttgctga tggcggcggt atgtatctac tgattaacac taatggttct aaatactggc
    54361 gcatgaagta ccgctttgct ggtaaagaaa agatgctttc tattggcgta taccccgatg
    54421 taacgctagc cgatgctcgc gagaaacgca gtgaagccag gaaactcctc gctgctggag
    54481 gcgatccagg ggaggcaaaa aaggaagaaa aaatcgcgca gcaaataagt ctgaaaaata
    54541 cgttcgaggc tattgctcgc gaatggcacc agtcaaaagc cgatcggtgg tcattacgct
    54601 atcgtgatga aatcattgat acctttgaaa aagacatttt tccctacatt ggcaagcgcc
    54661 ctattgccga aatcaaacca atggagttac tggaagcact gcgtaaaatg gaaaaacgtg
    54721 gagctctgga gaaaatgcgc aaggtccgcc agcgctgtgg tgaggtgttc cgctatgcaa
    54781 ttgttactgg ccgggcagac tataacccag cccccgatct tgccagtgct ttagctacac
    54841 cgaaaaaagt acattttccc tttcttactg ctaatgaact tccccacttc ctcaacgatc
    54901 tggcgggtta taccggaagt ataatcacta agacagctac tcagatcatt atgttaaccg
    54961 gcgtacgaac acaagaactg cgttttgcac gctgggaaga tatcgatttt gagacaaaat
    55021 tgtgggagat cccggcagaa gtaatgaaga tgaagcgacc tcatattgtg ccgctctctg
    55081 agcaggttat tatgctattt aagcagcttg aaccaatctc aaaacatcat cccctagtct
    55141 ttatcggtag aaatgatccc cgcaaaccaa ttagtaagga aagcattaat caagtaattg
    55201 aattacttgg atataaggga agactcacag ggcacggttt cagacacacc atgagtacaa
    55261 ttctgcatga acaaggcttt aactctgcct ggattgaaat gcagttagct catgtggata
    55321 aaaattccat caggggtacc tataatcatg ccctgtatct cgatggccgc cgtgaaatga
    55381 tgcaatggta cgctgattac attgattcac tctccagccg ggaaagttaa gtcagaatta
    55441 cttttccctg atgttttcct caattaagta ttttatcctc tggcctacaa ggctggagga
    55501 tttttgttat tatattattt ttttgcgtct tcaaacttat tacaaaaaca taattaaaaa
    55561 caaattgaca tttttaaatt ggcaattaca aaatacagcc ttgtagttat ttcgatacct
    55621 ttagcaaagg aaaagttgat tcagatataa tttagaattt caatcatctg acatcattat
    55681 aaaaaatgag attcacataa agataaaatt attgttctca aggaaagcat gaaggggctt
    55741 cgtaagcaat attcattcgg tgccaggaca ggacttgata cacagaatct caacttttat
    55801 gctctcagac tatatcacca catgttttac aggctcagaa aatgaacgat agattatgct
    55861 ttgaagtgca cgataacaag ggttatttcg tatttccgga tacctggttt ggcccattgc
    55921 tgggggagtt tgaagaagtg cttgatgcat acgacgctga tgaaatcagc gagacgagtt
    55981 acatcaacaa actccgacgc cttgctcaac gggagccgga ctttattgat atacacgctc
    56041 atctggcata tgcatttctg gaacagaatg ccccgcgtaa ggcactgaac gccgctctta
    56101 aaggactggc ggcaggtaat cgtatcatcc cggaaagttt ctgtggtgaa ataatctgga
    56161 tgcatcccga aaaccggcct tatctgcgcg cgctttatgc agcaattctc gcaaatgtgc
    56221 acttacagcg ccatcaggat gcggttatgc tcactgataa aatactggca tacaatcctg
    56281 aagataatca gggggctcgc tggttgttgg gttcagagct gttacgcacc ggcgatcatg
    56341 agcgagcctt cagtgtactg aaagagcatg ctgatgaatt ttcaccttac tggtatgaac
    56401 tcggtctcct gcattttctg aacggggaac atgttaaggc cgccacagct tttcgccatg
    56461 gctttgccac aaacacttac atcgcagaaa tgctgtgcgg aaatctgcat ccatttccac
    56521 ttgctgtgtg gcatgacttc tccggctctc tggatacggc agaagattac tatgcaacat
    56581 actcaccatt atggggacaa tatccggaag cattgctgtt tgtaaactgg ctctataacc
    56641 attcctccgt gctgcatgaa cgagcagaaa tcattaaatg cgccgaaatg ctgattcagg
    56701 aagatgattt cgaaatatgc gaaagtattt taagacagca ggagcatctg tggaaaagaa
    56761 ttgacaagac actttctgag gaaattgtac agaaatgcag aaatatgaac ggtgaatata
    56821 tctggccctg gatattgccc ttttccgcag tagggatgaa acattcaagc atccagcatc
    56881 agtagatatt gcattagtgt aactttaaaa caagtaataa tttattatcc aataataaca
    56941 ctgtgttcct tccccaggtg attaccctgg ggttattttc tgaaagtttc ctttgcaaaa
    57001 ggatagcgtt gtaaattact ttaacgaatt actccatgaa ctcattaaag ttctttcgta
    57061 atactttatc gtcaaacacg ttatagctgg catcaaaaac cacagattta ccctgccagc
    57121 gggtaatccc ctcctctggc gttttcggtt gaactggtat ccccgtccaa caaaggcagc
    57181 cattataaac agtaaaaaat tgtattcgct gttatgaaat caatagtcct gacgtactgt
    57241 tgaatgtgct tgtgttctga acctcgtaca accagtacat ttgatatcga attataaaca
    57301 aaacattgca tccaactttt tcaatatgga caatagttgt ccatacggtg gttttatatt
    57361 cgataaatca atggatttca ttgagtcaca ggactggtaa tggatgaaaa tgctttaggg
    57421 tttgcctcat actggcgcaa ctcgcttgcg gatgctgagt caggaaaggg cagttttgaa
    57481 cggaaagacg ccaaaaattt cactcactgg catgggatag cggcgggacg tcttgacgaa
    57541 gcgattgtca gtaaattttt taagggagaa aaagacgatg tagaaacggt cgatgtcatc
    57601 ttgcgcccaa aagtttattt tcggttactg cagcatggta aggaccgttc tgcaggtgcg
    57661 cctgatattg ttaccccgat agtgacgcca gccttgctaa gccgtgaggg ttttttatat
    57721 ccgacgccag cgacctccat tcccagagac ctgcttgaac ctttgccaaa aggagcattt
    57781 tcgattggtg agattgggca gtatgacaaa tacaagacga cccataccac gttctctatc
    57841 aactttgatg acagcgttga taagactgcc gaaacggatg aagaacggga agcacgatat
    57901 gccgccttgc agcaggagtg gcgtcaatat ctgtatgact cagagaggct actgaagagc
    57961 gttgccggcg actggattga aaaacctgag caatatgaac tcgctgagca cggttatatt
    58021 gttaaaacgg ctcaatctgg cggtgccagt ttccatatcc tttctcttta tgatcacctg
    58081 cttgtttgca ataaggatgt gccgctcttc aatcgattcg cctcgcgaga ggttcatgct
    58141 gcagagtctt tgctggcccc tggagcaaaa ttcagcgaca ggcttggaca ctccggagat
    58201 aagtttccgc tggcaaaggc tcagcgcgat gccttaagcc attttctgga tgccagacat
    58261 ggcgatatcc ttgctgttaa tggccctccg ggaaccggaa aaaccacgct ggtgctttct
    58321 atcatcgcca cgcagtgggc cagagcggct ctcgaaaaat ctgagcctcc ggttattatc
    58381 gcgacttcaa cgaataacca ggctgtaacg aacattattg aggcattcgg gaaagacttt
    58441 tcgcaaggtt caggtgcgat ggccgggcga tggttgccag agctgaaaag cttcggcgct
    58501 tattttccct caagcagtcg taaagctgag gcagccaaaa aatatcaaac tgaagatttc
    58561 ttcaaccagg ttgagtcaaa agagtatgta gaggatgcac tgctgtttta tctggaaaag
    58621 gctaaggcag cctttcctgg aaaagagtgt tcatcccctg aaaaggtcat tgaactcctg
    58681 catggtcagt tggcagcaaa atctgagcaa ctgataagac tgaacgcaac atggcaaacg
    58741 ttaagccaga ttcgggctgc gcgtgagctt attgctaatg atattgagca atatctcaat
    58801 aatttaaata aattactttc cggacaagaa caaaaagtca ctctactgaa gagtgctaaa
    58861 acggaatgga aaaaatatcg cgccggtgaa tcactgatct attcattatt ttcctggctc
    58921 ccggcggttc gcaataagcg acagtaccaa atacagctgt ttctcgaaga taaattaggc
    58981 gcgctgattg caggaaatca gtggtctgat cctgaaacta tcgaacgtaa tattgatggg
    59041 ctgctcaatt ccgctgagcg cgagcaaaca acataccggc agcagattga ctccgcccat
    59101 gaaatcgttc ttaaagaaca gcaggcggtt caggagtggc agaggctggc atttgattta
    59161 gggtatgagg gcgacgagga actgagcttc tcacaggccg atgaactggc tgatacgcag
    59221 attcgcttcc ctgcattttt actgacgact cactactggg aaggtcgttg gctgatggat
    59281 atggccagaa ttgatgatct gcaggacgag aagaagaaaa aaggtgctaa aggggtaacc
    59341 gcccgttggc aacgtcgaat gaaactcacg ccatgtgtgg tgatgacatg ctatatgctg
    59401 cccggcaata tgcagataag tgagcacaaa ggacaacgta aattcgagaa aagttatttg
    59461 tatgattttg ccgatttact cattgtcgat gaagccgggc aggtgcttcc tgaagtggct
    59521 gctgcctcgt ttgcattagc taagaaggca ttagtgattg gcgatacgga gcagatcccg
    59581 ccaatatgga gtattgctcc cgcgattgat gtcggtaaca tgctggagga aaaaattctg
    59641 tcgggcagta cgcaagaaga gattaccgag aaatatacgg caatcgcaga ccttggtaaa
    59701 agtgccgcat ctggcagcgt tatgaaaata gcgcagtttg cttcgcgcta tcaatatgat
    59761 cccgaactgg ctcgtggtat gtacctatat gaacaccgcc ggtgctacga caatattatt
    59821 ggatactgta atacgctctg ctatcacggt aagttgttgc ctaaaagagg gcgtgaaaag
    59881 agcaatttaa tgcccgcaat ggggtatctc catattgatg gtaaaggaga gctggcaagt
    59941 agtggaagtc gatataattt gcttgaggct gaaacaatag cggcctggtt ggcagagaac
    60001 cagcaaaata ttgaagcgca ttacggcaaa tcgcttcatg aagttatcgg tatcgtgacg
    60061 ccttttagcg cgcaggtatc cactatcaaa caggcgctgg gtaaacaagg tatcagtact
    60121 ggcgcgaatg aaaagtcgct cacagtgggc accgtgcact ctcttcaggg agcggaaaga
    60181 gcgattgtgg tattctcgcc agtctattca aaacatgaag acggcgggtt tattgatagc
    60241 gataacagca tgctgaatgt tgcagtctcc cgtgcgaagg atagcttcct ggtcttcggc
    60301 gatatggatc tgtttgaggt ccagccgccc tcatctccgc gaggattgct ggcaaaatac
    60361 ctctttgagt cagagaagaa tgcgctctct tttgattata aagagcgtaa ggatttaaaa
    60421 acctccggga ccaaaatcta cacacttcat ggtgtggagc aacatgataa attcctgaat
    60481 cagacatttg aaaataccag taaatacatc acgatagttt ctccatggct gacctggcaa
    60541 aagctggagc aaaccggttt tcttgattcc atgattgcgg cgtgttcacg tggtattaac
    60601 gtcacgatag tcactgacag aagctacaac actgaacata aagattatga gaaacgaaaa
    60661 gagaagcagc agaatcttaa agggacgctg gaaaaactga atgcttttgg tattactaca
    60721 aagctggtaa atcgcgttca tagcaaaatt gttattggtg atgatggttt gctgtgcgtg
    60781 ggatcgttca actggtttag cgcaacgcga gaagcgcgat atgaacgata cgatacctca
    60841 atggtttatt gcggtgataa cctgaagggt gagattgagg ctatttataa tagtcttgag
    60901 aagcgtcagg tttagagcag gaatggtgag tagccatctt atcgatcgtt ttcgagcgta
    60961 agatggctga atggaatagc tattattgca cagtccttaa ttataacatt cataccaaca
    61021 tgattatctt ctgtccggaa gaatcaaagg ctgcggtttc agactgtctg ccggcacatt
    61081 tttctctccg ttaaaaacca taacgggttc attatcttcg tctgtcagcg gattgaatgg
    61141 cggtatattt tcggtacgaa taccggtcag ccactgaaaa atgcctgtga aatgacgggc
    61201 actgattttt ctgctgacgg actgatgaga cgtgatgtca ctggcggtat taatcagggg
    61261 aacgctgtag cctccctgca catgaccatc atgatgaaca ggattagcac tgtcgctgac
    61321 cgacagccca tggtcagaaa agtaaagcat agcgaaatga cgggaatgcc ggcgaaggat
    61381 accatcaaac tgaccgagaa agttatccag tttactgatg ctggcgaggt aacaggcaat
    61441 ctttcgggga tactgctcca ggtaatgatt cggccaggag ttaagtcggt cacacggatt
    61501 cggatgagag ccaatcatgt gcaggaaaat caccttcgga gaggatttat ccgccagcgc
    61561 acgttctgtt tcctgtaaca acaacatgtc atccgtttta cgggaagcaa agctgccttt
    61621 cttgaggaaa acggtatgct ccgcatcaga agcaataaca gagatgcgtg tatcatgctc
    61681 ccccagtttt ccctgattgg atatccacca tgtgctgtat cctgcttttg ctgccagcgc
    61741 caccacgttg ttgccggagt cagggttctg ctcatagtca taaatcagtg tccggctcag
    61801 ggaaggtacg gtactggctg ctgtcgatgt atagccgtca ataaataaac cgggagcagt
    61861 attcagccac ggcgtggttg gcacgggata gccatatacc gacatataat ccctgcgcac
    61921 actctcacca gtaacgataa caatcgtgtc atacaacggc acacccggca ggattttcca
    61981 gttgtcagcc ccgtgctgac tcagttgttt ataacgctgc atttcacgca atgtgtcagt
    62041 tgcccccaca acagttcctt taaccatccg caacggccag ctgtttacag agcataatac
    62101 gaatagcagc agtgccagcc agttacggtg accgcggtgg tgtgcccgcc agaaaatcac
    62161 tatgaatacc agaatcgcgg cactgaccag aaaatgataa acaggaatca tcccggtaaa
    62221 ctctgcagcc tcatcagccg tggtctgcag caacgcgaca ataaaactgt tgttgatttt
    62281 accgtacgtc atacccgcag gcgcatacag tgcacaacag aacagaaata acagcgctgt
    62341 aatggatgtg agggtatttc tgtgtgcaag gagaagaagg agaaacagaa gcagcacatt
    62401 tcctgtggca ttcttctcag tgtatccgca tgcaattgag gttattgcag aaacaacaaa
    62461 aaagaataaa aacaataaaa tccggggtgt gttgcccgga caaaacaatt tcctgatatt
    62521 catcggagta tatcgacaac attattatga agagaacaag ataataaaaa tcagaaatta
    62581 tctgtaaaac agataacaga caaccctgca gtaatagact gcagggtatt gctgtttaat
    62641 tgcagaagat atgcaattat cttaattaca gaatatacgc aattatcgtt caggagcagt
    62701 gtcgtcagaa gttataagtc ataccaagca tgatgtcatg acttttaaca tcaacttctg
    62761 acttatattt atcgccccac tcgtccttat aactcacact ggcatcacct gcatcaagat
    62821 agcgatagct gaggtccagc gcgatatccg gggtgacgtc atagcggaca cctgcgccaa
    62881 agctccatgc aaagttatca gcagagcctg agcgtgacaa cgattcgcga ccactgtttc
    62941 cgtacccatg atcccagata ctgataccgg ttgttttctg gtgaactctg gcgtagccaa
    63001 tccctgcaga tatccatggt gtgaatgcgc tgtcattccg gaagtcatag tacgtattca
    63061 gcatcagtgt gttgactgac acttcattct tcaggtcatc acgccagtaa ccacctgacc
    63121 aactgtcttt atctacgtta tacttcgaat cagcttttcc acgagcgtaa aactccagtt
    63181 ccgtacgaac cggaatactg aactgcggat aaaaatcata accagccgcg ataccgccac
    63241 tgaataccgt atcatggtca ttaccacctt tatatttgtc cgcccactct ccttcaccat
    63301 cgataagacg ctggttggaa agagataaaa cggaagtccc cgccttaccg gtcatataaa
    63361 agcctgtttt gctctcatca gccaaggcct gggctgaaaa catacccgcc attgcaaata
    63421 ctgaaaccac aataaccttt tttatttcaa tcatctccgt tccctacccg acaaagaggc
    63481 tcacctattc taaaaccatg caaaagacag ggtaaaaaga gaaatttcga tcgaatcgat
    63541 cgacaaaatc gatcatacat gatgaagatt tcttatcgaa tccataaaaa tagtgacagg
    63601 taacgggtac tgcaggaaca atcagaaatg gaagtttggg aaagtgccat agcactacgt
    63661 cgttgctaac atagaggaac agtgctttgt tgataagatc ctttatacga cgaatctatt
    63721 ggatgaaaaa taaactacgt aatcacctga ttctcactga tattgccaat tctggtagga
    63781 ttgagtgatt cttcgcttac aaaacgatgc tcaggaatat gataattgtt atatatcgat
    63841 gtatacagga caatattagt ggatctcagc atgaattata taaagtggat ttacccacgc
    63901 cgcttacgca atcaaatgat cctgatggca atcctgatgg tcattgtccc aacgcttgct
    63961 attggttata tcgtagaaac ggaaggacgt tcagcagtct tatctgaaaa agagaaaaaa
    64021 ctttctgccg tggtcaacct gcttaatcag gcgctaggcg atcgctacga tctctacatc
    64081 gacttaccgc gtgaggagcg tatccgcgca ttaaatgcag aacttgcccc cattaccgaa
    64141 aatatcactc acgccttccc tggcatcggc gctggttatt acaacaaaac gctggatgcg
    64201 ataatcacct acgcgccttc agcgctatat cagaataatg tgggcgttac cattgccgca
    64261 gatcaccctg gtcgcgaagt catgcgtaca aatacccctt tggtgtattc aggcaggcag
    64321 gtgcgcggcg atattttgaa ttcaatgatc cccattgagc gtaatggtga aatcctcggc
    64381 tatatctggg ccaatgaatt aaccgaagat attcgccgcc aggcctggaa aatggatgtg
    64441 aggattatca ttgtgctcac cgccggtttg ctgataagcc tgctgttgat tgtccttttc
    64501 tcccgtcgcc tgagcgccaa tattgatatc atcaccgatg gcctctcgac tctggcacaa
    64561 aatattccca ctcgattacc acaattgccc ggtgaaatgg ggcaaatcag tcagagtgtt
    64621 aacaacctcg cccaggcact gcatgaaacg cggacactta acgatctgat tattgaaaac
    64681 gctgccgatg gcgtcattgc cattgaccgt cagggtgatg taaccaccat gaacccggca
    64741 gcagaagtca tcactggcta tcaacgtcag gaactggtag ggcagcctta ctccatgttg
    64801 ttcgacaata ctcagttcta cagtccagta ctggatacgc tggaacatgg caccgaacat
    64861 gtagcgctgg agatcagttt tccaggccgt gaccgcacca ttgaactcag tgtcactacc
    64921 agtcgaattc ataacacgca tggtgaaatg ataggtgctt tggtgatttt ctctgattta
    64981 actgcccgca aagaaaccca gcgccgcatg gcgcaagcag aacgcctcgc cacactgggt
    65041 gagctgatgg ctggcgtcgc gcatgaagta cgtaatccgc taacggctat tcgtggttat
    65101 gtacagatct tgcgccaaca aaccagcgac ctaatacatc aggaatatct gtccgtagta
    65161 ctcaaagaaa tcgattcaat taacaaagtt attcagcaat tgctcgaatt ttcacgtcca
    65221 cgccacagtc aatggcaaca agtcagcctc aatgcattgg ttgaagaaac tctggtactg
    65281 gtacaaaccg ccggcgtaca agcgcggatc gacttcataa gcgaactgga taatgaatta
    65341 agcccgatta acgccgatcg tgaactgctc aaacaggtac tactgaatat cctgatcaat
    65401 gccgtccagg ctatcagtgc acgagggaaa attcgcattc gaacctggca gtacagcgac
    65461 tcacaacagg ccatttcgat agaggacaac ggctgtggca ttgatctctc gctgcaaaaa
    65521 aagatcttcg atcccttttt caccaccaaa gcttcaggaa ccgggcttgg tctggcgtta
    65581 agtcaacgca tcattaatgc ccatcagggt gatattcgcg tcgccagttt gccgggctac
    65641 ggcgcaacct tcacccttat tttaccgatc aatccgcagg gaaatcagac tgtatgactg
    65701 ctattaatcg catccttatt gtggatgatg aagataatgt tcgccgtatg ctgagcaccg
    65761 cttttgcact acaaggattc gaaacacatt gtgcgaacaa cggacgcaca gcattacacc
    65821 tgtttgccga tattcaccct gacgtggtgt tgatggatat ccgcatgcca gagatggacg
    65881 gcatcaaggc actcaaggag atgcgcagcc atgagacccg gccccccgtt attctgatga
    65941 cggcctatgc tgaagtggaa accgccgtcg aagcgctacg ctgcggggcc ttcgactatg
    66001 ttattaaacc gtttgatctc gatgagttga atttaatcgt tcagcgcgct ttacaactcc
    66061 agtcaatgaa aaaagagatc cgtcatctgc accaggcact gagcaccagc tggcaatggg
    66121 ggcacattct caccaacagc ccggcgatga tggatatctg caaagacacc gccaaaattg
    66181 ccctttctca ggccagcgtc ttgattagcg gtgaaagcgg caccgggaaa gagttgattg
    66241 ccagagcgat tcactacaat tcgcggcggg caaaggggcc gttcattaaa gtcaactgcg
    66301 cggcgctgcc ggaatcgttg ctcgaaagtg aactgtttgg tcatgaaaaa ggtgcattta
    66361 ctggtgcaca aaccttgcgt cagggattat ttgaacgtgc caacgaaggt actctgctcc
    66421 tcgacgaaat tggcgaaatg ccgctggtac tgcaagccaa attactacgc attctgcagg
    66481 aacgggaatt tgaacggatt ggcggccatc agaccataaa agttgatatc cgcatcattg
    66541 ctgccaccaa ccgtgacttg caggcaatgg taaaagaagg caccttccgt gaagatctct
    66601 tttatcgcct taacgttatt catttaatac tgccgcctct gcgcgatcgc cgggaagata
    66661 tttccctgtt agctaatcac tttttgcaaa aattcagtag tgagaatcag cgcgatatta
    66721 tcgacatcga tccgatggca atgtcactgc ttaccgcctg gtcatggccg ggaaatattc
    66781 gagagctttc caacgttatt gaacgcgccg tcgtgatgaa ctcaggcccg atcatttttt
    66841 ctgaggatct tccgccgcag attcgtcagc cagtctgtaa tgctggcgag gtaaaaacat
    66901 cccctgtcgg agagcgtaat ttaaaagagg aaattaaacg cgtcgaaaaa cgcatcatta
    66961 tggaagtgct ggaacaacaa gaaggaaacc gaacccgcac tgctttaatg ctgggcatca
    67021 gtcgccgtgc attgatgtat aaactccagg aatacggtat cgatccggcg gatgtataac
    67081 gccaaaactt gctatgcaga aaattgcaca gtgcgcaatt ttctgcatag ccgctcatta
    67141 tccttataaa tccccatcca atttatccct tcatattcaa ttagttaaat aacaaaatcc
    67201 agtaatctca ttctggcact ccccttgcta ttccctgact gtacccacaa cggtgtatgc
    67261 aagagggata aaaaatgaaa acaaaattga tgacattaca agatgccacc ggcttctttc
    67321 gtgacggcat gaccatcatg gtgggcggat ttatggggat tggcactcca tcccgcctgg
    67381 ttgaagcatt actggaatct ggtgttcgcg acctgacatt gatagccaat gataccgcgt
    67441 ttgttgatac tggcatcggt ccgctcatcg tcaatggtcg agtccgcaaa gtgattgctt
    67501 cacatatcgg caccaacccg gaaacaggtc ggcgcatgat atctggtgag atggacgtcg
    67561 ttctggtgcc gcaaggtacg ctaatcgagc aaattcgctg tggtggagct ggacttggtg
    67621 gttttctcac cccaacgggt gtcggcaccg tcgtagagga aggcaaacag acactgacac
    67681 tcgacggtaa aacctggctg ctcgaacgcc cactgcgcgc cgacctggcg ctaattcgcg
    67741 ctcatcgttg cgacacactt ggcaacctga cctatcaact tagcgcccgc aactttaacc
    67801 ccctgatagc ccttgcggct gatatcacgc tggtagagcc ggatgaactg gtcgaaaccg
    67861 gcgagctgca acctgaccat attgtcaccc ctggtgccgt tatcgaccac atcatcgttt
    67921 cacaggagag caaataatgg atgcgaaaca acgtattgcg cgccgtgtgg cgcaagagct
    67981 tcgtgatggt gacatcgtta acttagggat cggtttaccc acaatggtcg ccaattattt
    68041 accggagggt attcatatca ctctgcaatc ggaaaacggc ttcctcggtt taggcccggt
    68101 cacgacagcg catccagatc tggtgaacgc tggcggacaa ccgtgcggca ttttacccgg
    68161 cgcagccatg tttgatagcg ccatgtcatt tgcgctaatc cgtggcggtc atattgatgc
    68221 ctgcgtgctc ggcggtttgc aggtagacga agaagccaac ctcgcgaact gggtagtgcc
    68281 tggaaaaatg gtgcccggta tgggtggcgc gatggatctg gtgaccgggg cacgcaaagt
    68341 gatcattgcc atggaacatt gcgccaaaga tggttcagca aaaattttgc gccgctgcac
    68401 catgccgctc actgcgcaac acgcggtgca tatgctggtc actgaactgg ctgtctttcg
    68461 tttcattgac ggcaaaatgt ggctcaccga aattgccgac gggtgtgatt tagccaccgt
    68521 gcgtgccaaa accgaagctc ggtttgaagt cgccgccgat ctgaatacgc aacggggtga
    68581 tttatgattg gtcgcatatc gcgttttatg acgcgttttg tcagccggtg gcttcccgat
    68641 ccactgatct ttgccatgtt gctgacattg ctaactttcg tgatcgcgct ttggttaaca
    68701 ccacaaacgc cgatcagcat ggtgaaaatg tggggtgacg gtttctggaa cttgctggcg
    68761 tttggtatgc agatggcgct tatcatcgtt accggtcatg cccttgccag ctctgctccg
    68821 gtaaaaagtt tgctgcgtac tgccgcctcc gccgcaaaga cgcccgtaca gggcgtcatg
    68881 ctggttactt tcttcggttc agtcgcttgt gtcatcaact ggggatttgg tttggttgtc
    68941 ggcgcaatgt ttgcccgtga agtcgcccgc cgagtacccg gttctgatta tccgttgctc
    69001 attgcctgcg cctacattgg ttttctcacc tggggtggcg gtttctctgg ctcaatgcct
    69061 ctgttggctg caacaccggg caacccggtt gagcatatcg ccgggctgat cccggtgggc
    69121 gatactctgt tcagtggttt taacattttc atcactgtgg cgttgattgt ggtgatgcca
    69181 tttatcaccc gcatgatgat gccaaaaccg tctgacgtgg tgagtatcga tccgaaacta
    69241 ctcatggaag aggctgattt tcaaaagcag ctacagaaag atgccccacc atccgagcga
    69301 ctggaagaaa gccgcattct gacgttgatc atcggcgcac tcggtatcgc ttacctggcg
    69361 atgtacttca gcgaacatgg cttcaacatc accatcaata ccgtcaacct gatgtttatg
    69421 attgcgggtc tgctgctaca taaaacgcca atggcttata tgcgtgctat cagcgcggca
    69481 gcacgcagta ctgccggtat tctggtgcaa ttccccttct acgctgggat ccaactgatg
    69541 atggagcatt ccggtctggg cggactcatt accgaattct tcatcaatgt tgcgaacaaa
    69601 gacaccttcc cggtaatgac cttttttagt tctgcactga ttaacttcgc cgttccgtct
    69661 ggcggcggtc actgggttat tcagggacct ttcgtgatac ccgcagccca ggcgctgggt
    69721 gctgatctcg gtaaatcggt aatggcgatc gcctacggcg agcaatggat gaacatggca
    69781 caaccattct gggcgctgcc agcactggca atcgccggac tcggtgtccg cgacatcatg
    69841 ggctattgca tcactgccct gctcttctcc ggcgtcattt tcgtcattgg tttaacgctg
    69901 ttctgacggc aaccctacag acagaaggaa taaaaaatga aaaattgtgt catcgtcagt
    69961 gcggtacgta ctgctatcgg tagttttaac ggttcactcg cttccaccag cgccatcgac
    70021 ctgggggcga cagtaattaa agccgccatt gaacgtgcaa aaatcgattc actacacatt
    70081 gatgaagtga ttatgggtaa cgtgttgcaa gccgggttgg ggcaaaatcc ggcacgtcag
    70141 gcactgttaa aaagcgggct ggcagaaacg gtgtgcggat tcacggtcaa taaagtgtgt
    70201 ggttcgggtc ttaaaagtgt ggcgcttgcc gcccaggcaa ttcaggcagg tcaggcgcaa
    70261 agcattgtgg cggggggtat ggaaaatatg agtttagcgc cctacttact cgatgcaaaa
    70321 gcacgctctg gttatcgtct tggagacgga caggtttatg acgtaatcct gcgcgatggt
    70381 ctgatgtgcg ccacccatgg ttatcatatg gggattaccg ccgagaacgt ggccaaagag
    70441 tacggaatta cccgagaaat gcaggatgaa ctggcgctac attcacagcg taaagcggca
    70501 gccgcaattg agtccggtgc ttttacagcc gaaatcgtcc cggtaaatgt tgtcactcgg
    70561 aagaaaacct tcgtcttcag tcaagacgaa ttcccgaaag cggattctac ggctgaagcg
    70621 ttaggcgcat tgcgcccggc cttcgataaa gcaggaacag tcaccgccgg gaacgcgtct
    70681 ggcattaacg acggtgctgc cgctctggtg attatggatg aatctgcggc gctggcagca
    70741 ggccttaccc ccctggctcg cattaaaagt tatgccagcg gtggcgtgcc ccccgcattg
    70801 atgggtatgg ggccagtacc tgccacgcaa aaagcgttac aactggcggg gctgcaactg
    70861 gcagacattg atctcatcga agctaatgaa gcatttgctg cacagttcct tgctgtaggg
    70921 aaaaccctgg gctttgatcc tgagaaagtg aatgtcaacg gcggggccat cgcgctcggg
    70981 catcctatcg gtgccagtgg tgcacgtatt ctggtcactc tgttacatgc aatgcaggca
    71041 cgcgataaaa ccctgggcct cgcaacactg tgtattggtg gcggtcaggg gattgcgatg
    71101 gtgatcgaac gattgaatta acagcagaag ccacctccag tccataggag gtggtttcac
    71161 aggcctcata aaaactcgtc agagtgtaac ttgagtcctt actgaaacag gattagagca
    71221 gaagatgttc atttgtaaat ataattggag agacgaacag caattagaga gagtcgcctc
    71281 ctcccagtta tttgtataat tcataaacac catctcaaat tcctcacatc aaaatccatt
    71341 acgttgctgg aaacagcaaa ggttcaggct tatttatccg tctctgttaa aacatcagaa
    71401 agcaacgtac cgatgggcaa aacgattaca ttcattgtca ataacttata tcatccacaa
    71461 aaggccgcga aacgttgcgc tggttgaagg aaaatcgtaa gcgtcaacag agcgccgtat
    71521 tgacacttat ttatcggtga taaactacgt tccatggcag cagttcgtca acacggttgg
    71581 agggccattc cggcagtacg ctcaggatat ggcgcagata cgcttctgga tcgataccgt
    71641 tcaaccgaca gctcccgatt agtccgtaca gcagagctcc acgctcgcct ccatgatcgt
    71701 tgccgaagaa catgtagatg gtatgacctt tatatactca ctatagcatt ggtgggatat
    71761 tccagaggag gtcattatgc attcggaaaa tattgcagct tacatcgggc tggatgtaca
    71821 taaagaaacg ttggcggttg ctattgctgc acctgaacgg cttggcgaag ttcgttatta
    71881 tggtacgatt aacaacgaag cccaggctgt acgtcgatta tttcagaaat tgcagggact
    71941 ttacggaaac attctttcct gctatgaggc tggcccctgt ggttttgggc tatatcatca
    72001 actcacagcg atgaatataa aatgccaggt tattgcccct tcccggatac caaaatcacc
    72061 gacagacaga atcaaaaatg atcatcggga cgcaatttct cttgccaggt tactgcgggc
    72121 tggtgaactt acccctgtct ggatccctga cctgactcat gaagctatgc gggatctaat
    72181 cagagcccgt gcagcagcta aacgagacag cagagttgcc cgacagcgaa ttctgagcat
    72241 gctattgcga acagacaaac gttatgcagg gaagcactgg accggcaaac atcgaacctg
    72301 gttagccaat caatcattta gtcagccatc tcaacagatt gcttttcaac actattgtca
    72361 gagtctggag cagattgaag atcgcatact tcaactcgac caggaaatat cccgcctgtt
    72421 acctgagtgg tctctgtgca atctggtctg ccagttacag gcccttaaag gtgttggtca
    72481 gctaaccgca ataaccctgg ttgctgaact gggtgatttt tcacgatttt ccagtcccaa
    72541 acaactcatg gcttttctgg gactggttcc aggggaatat tccagtggaa acagcattcg
    72601 tcccagggga ataacaaaag ttggaaacag cgaactgaga cgtctgcttt acgaagccgc
    72661 ctggtcttat cgtacacctg caaaagttgg tgcatggctt atatattacc gaccggattc
    72721 tgtaacacaa tattccaaag atattgcatg gaaagctcaa caacgattgt gttctcgtta
    72781 ccgaagtctg acagcaaaag ggaaaaaatc acaagtagcc attacggcgg tggctcgtga
    72841 gttgactgga tttatgtggg atattgcact tgctgcccaa tcatcattca gtcagcagaa
    72901 gcaaaattaa accctgaagc agtcagacac ggaatgagtg atcctcgtaa aagaattcag
    72961 gaggtcagaa tgcgccaata cctgcatata gaaagaggcc agctcactgc gtaaatcgtc
    73021 atggggctaa aatccccgaa taagagtttg taaaatccaa gatacagtct gactgtttca
    73081 ggttctttaa tccgcgatat aacgaagctc ccggagctgc gtaaggtctt tttgggctca
    73141 atatttgaca aaggccataa gaattctttt tcccgagaca gacggcacga agcgctcttt
    73201 ctgctgtgtt attgtccgcc tccgccagac cgtcatcact gtaataacag agggcgtccc
    73261 actgattcag gacatagctg aacgcttcgc ccagtctgga ttttttcgac aacgtgccat
    73321 tcttctccac catccattca tgcagcgacg tcagtaacgc tttgcttcgc tgctgcctgg
    73381 ctgcaagacg ttcagactcc ggtaagcccc gtatttcatc ctcgatggcg tacagttcac
    73441 tgatgcgctt cagagcttct tctgccgtcg tacttttgct gctgatgtat acatcgtgga
    73501 tttttcgccg ggcatgggcc cagcacgcaa cttctgtcag tgcaccacct tcacgttcgg
    73561 cactgaacag ccgctcgtaa ccgctgaacg catccacctg caggataccc cggaagggac
    73621 gaaggtgttg gtcccgggac tactttaggt cttggttcac taataatttc attactgtca
    73681 atagccgtaa tcgccattgc aggaaataat aaaaagtaag tgtactctga ctgcgtatgt
    73741 atctactttg tgtaatgcag gtgtctattg aaaataattc ttttcgtggg catctgttgt
    73801 ataaaataaa aaattatcgt aagtactcca tctgcgatgt cttgtgaaaa ttatcctgtc
    73861 tggcaacaat cgcgtccatc tgtattaatg gactcgaacg atgaactctc aaacaacact
    73921 agatatcacc ggctttcgca cttatttgcc agactcatta cgtctcagat tcgacgagca
    73981 attatctgtc cggactatcg cacagcaagt taagactcag ttattccccc attcacaatc
    74041 tctttcaacg aattaacgcc tcaggtattg tctgaccatg acccgagtcc ctttcattcg
    74101 cccaaccgat accatacttt atgccaacag aaagaatgaa tgaaccgcgc ctaaaatcag
    74161 cgaggacaca tggcacaaag tgcgacatgt ctcacattta accccagtgg gggcaacaag
    74221 tataaaaccg ttttttgatt aagtggtcgg atatgaaaag gggaaggttt atgtgaaaaa
    74281 tacctgtgtt ttctttgaaa acacacaaca gtatcaggtt atttatgttt atacagtgcg
    74341 ttccaaactg acctgaataa ttacgcaagt tgcaacaata aaaacatttt taccgtagtg
    74401 tgaggtactc cggtaaattg ctttccaggt gcagacaatg actaacgaag aactacaggc
    74461 aatcgcatta tccatgcttg agcgccagcg cgaaaaagta caccagcaac gagagtcttt
    74521 gcttaaccca tctccattca ccaggccaaa acgattggag cttgaagcat tcttggacaa
    74581 atacccacgg cggcttaaat caggcaaaac cagaccacca ggctgatagc tcacagtttg
    74641 ttctcctatt caataaaacg gataagaacc atcagcgcgg tagcgtaagg cactacagcg
    74701 cattgcttta cgagtaataa caatggattt atcaccagaa gacgcaaagt ttttacgaga
    74761 actgcaacac cgtatgctca gagaagccat tatcgaacag caaacgggca tgagcgccat
    74821 agacccattg cgatacaaac gggcctcatc tcctttggtt gacgacaatt ccaatgcgga
    74881 taaaatacgt ccaccacact gattacattt ctcattagca cagtggtgtg atgcagcata
    74941 cgaggaaaac cggatgaatg agaacagaat aaaaaggctg gagcaactgt tacaagcacg
    75001 ccaacgggag tttgccacta aatggggacg tgccgcgctg ccacatgaac gcctaatcta
    75061 ttttccactt gaagacctca ttaaaaataa tggcaaaaaa atcaggcctg ataagaaaca
    75121 cttcttagaa tgcaatgaag acaaataaca acaatctgca tattgccacc aaccacagat
    75181 gtggcatcgt tatcagcgcg cttgattttc tttatatcca ctgacgagct taccggggca
    75241 cctttgtttg ccggtcattc tggtatcatg cagatattta cggcgtacaa gcccactagc
    75301 atgacattgt aataaggcct gagcctaacg ctttaatgtg tgtcatacac tcagtggcgt
    75361 aatcgttgac aatatggtgc atgtaatcgt aagcgtccag cgaaatccat ctggtcataa
    75421 tctgaagcat ccgacaaagt ggtgtccacc aaataagtga tctgcgccca atatcctgag
    75481 ccggaacttt gacgaaggcc aacgaaaaat gggggcggat gttacagagt tctcggtaca
    75541 ggggtaaagt ggtacctgtc gccagttctc cgatctgttt aaccgggaaa ttatctccta
    75601 cagcttgtca gaaaggtcgg tgatggagca tcgttaatac gatgctatac gatgcattca
    75661 cagtgcccgg ccagaggatg ccccgtcgct gcatacggat cagggttggc aatatcgaat
    75721 cgcaggctat aggcaaagtt aaagccccat ggagtagcac aaaatatgcc gcacaaagga
    75781 aacggtctga ataacgcagt gatggtgaac ttcttcagca cactgctaaa acgcatagtg
    75841 atcgagcgct gattctggcg aacaactgaa ctaatacatc agaatctgca ttatgttaaa
    75901 taaatatgaa aagatggttt aaataccccg ttacttttga cttacactat acggtatcgc
    75961 atcgtttaat atccgcaccg accagatttt tatttctatt agttgtcaca atactgaatg
    76021 cgtacgacca cagtattccg gctcctgtgt ggtcatgctt taattctgcg ttccgggcag
    76081 ataagcagtt gcttgcagga atccttcttg tgttaatgtc agttcccctt tttaccagtg
    76141 ctgatttcca cattccgtcc aacagagctt atagccttag cattgtccgg ctgaagttct
    76201 ttttgaataa taatagaagc actgctggca gatccagtcc gtttttcata acccactgta
    76261 ctgataacca taatctaatc agtagaaatt gagtcgaaaa taagcactac tccatacagg
    76321 ataattagag gtcagtttga ttattcacaa ttcatcatca gcattttctg tttctgacga
    76381 aatcaacatg aaaa
//
LOCUS       DAZFKF010000013        72954 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFKF010000013 DAZFKF010000000
VERSION     DAZFKF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.013"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            160..756
                     /locus_tag="ACHKA1_001059"
     CDS             160..756
                     /locus_tag="ACHKA1_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5678747.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIEPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            843..1568
                     /locus_tag="ACHKA1_001060"
     CDS             843..1568
                     /locus_tag="ACHKA1_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO5678748.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVAPFSQASFGVTGLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            1610..4210
                     /locus_tag="ACHKA1_001061"
     CDS             1610..4210
                     /locus_tag="ACHKA1_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFO5678749.1"
                     /translation="MYQFTHQKSRIPKKTLLAACCALFYSGNSSAADTVEYDSSFLMG
                     TGASTIDVKRYAQGNPTPPGLYNVRVFVNGQATSSLDIPFVDIGENSAAACITHKNLA
                     QLHIKQPEQPVTLLAREGEEEDCLDLAKSYEKADVRFDGSDQFLDLTIPQAYVLKSYG
                     GYVDPSLWESGINAATLAYTLNAYHTSSDNDNSDSVYGAFNSGINLGAWHFRARGNYN
                     WTTDNGSDFDFQDRYLQRDIPAIRSQIIMGDAYTTGETFDSVNVRGVRLYSDSRMLPS
                     ALASYAPTIRGVANSNAKVTVTQNGYKIYETTVPPGEFVIDDISPSGFGSELVVTIEE
                     ADGSKRTFTQPFSSVVQMQRPGVGRWDFSAGKVIDDSLRSEPNMGQASYYYGLNNLFT
                     GYTGIQFTDNNYLAGLLGVGINTSIGAFAVDVTHSRAEIPDDKTYQGQSYRVTWNKLF
                     QDTGTSFNLAAYRYSTQDYLGLHDALVLIDDAKHLSADEDKNTMQTYSRMKNQFTVSI
                     NQPLKTTYEDYGSLFISGSWTDYWAANNSRTEYNVGYSKSVSWGSFSVNLQRSWNEDG
                     EKDDAMYVSVSVPIENILGGKRKSSGFRNLNTQLNTDFDGSHQLNVNSSGNTENNLVN
                     YSVNAGYSLDKNSGDLASVGGYLNYESGLGGISASASATSDNSQQYSISTDGGFVLHS
                     GGLTFTNNSFSSNDTLVLINAPGAKGARINNSNNEIDRWGYAVTSSVSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSVVLTRFETDEGRSAVLNITAANGKSIPFAAEVYQG
                     EVMIGSMGQGGQAFVRGINDSGELTVRWYENNQTVDCKLHYQFPAQPQTLGSTNTLLL
                     NNLTCQVANH"
     gene            4224..4772
                     /locus_tag="ACHKA1_001062"
     CDS             4224..4772
                     /locus_tag="ACHKA1_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5678750.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVNFTATVLATTCTITIVQD
                     GGPVVTGSNGEYSLTIPDVGLDKVVTGDVAAQANFKLVASDCSNGYSKIFTTLTGTTT
                     SGKLIVNEASSGATGVGMGIKRRDTADSTFFTPNNTDKFEWTADEKTNGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            4787..5401
                     /locus_tag="ACHKA1_001063"
     CDS             4787..5401
                     /locus_tag="ACHKA1_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5678751.1"
                     /translation="MKLKVIATLIATVAVGVSVSNNLTYASTTSASLTVNSNLTMGTC
                     SAKVVDTSGAVIDTVVLGNVYISELVAKSKVQPFKIRFSDCAGLPGKSAQLVLAPRAN
                     VGCAGGMSNTAAFSNQLNSTTDGGSSRAAVEVWTTTSPEGNGSEQFNCYQRNVINVDL
                     SNASTTQPYDFDLSARMIIAEGWSVNDVLPGRFLSPTTFTITYQ"
     gene            5422..6012
                     /locus_tag="ACHKA1_001064"
     CDS             5422..6012
                     /locus_tag="ACHKA1_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO5678752.1"
                     /translation="MLCRHHKFVRFLGLATALITPFAYAGQDVDLTAQIVASTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQVNNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            6024..7136
                     /locus_tag="ACHKA1_001065"
     CDS             6024..7136
                     /locus_tag="ACHKA1_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5678753.1"
                     /translation="MQRKGNKLLIQLCSAILLFFTTSWYALANECYIERNAEGPYDMV
                     INPKPFSIQSAMVTVPEEIGFATWDSYIDLRGDKIGCKSLGSNTSSVHFLNTADASLL
                     STYTTSHGDALLKTTVPGIVYSVELVCTSCGAAEELDLYLPAQSGADNHTDNASSKWA
                     YEYSDESWYLRFRFFWTPEFKPQNGVSEGTAIPGKIASWYIGNNDQPWINFFVDTSSV
                     HFYVDEPTCATVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKI
                     TVKLKAANKPSDATLVGKTNGSASGVAVKVNSTYDNSKVLLKADGSNTVDYNFAVWSN
                     NLLLLPFTAQLVPDGSGAAVGVGTFSGNATFSFTYE"
     gene            7272..8066
                     /gene="panB"
                     /locus_tag="ACHKA1_001066"
     CDS             7272..8066
                     /gene="panB"
                     /locus_tag="ACHKA1_001066"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO5678754.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALVIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8078..8929
                     /gene="panC"
                     /locus_tag="ACHKA1_001067"
     CDS             8078..8929
                     /gene="panC"
                     /locus_tag="ACHKA1_001067"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO5678755.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLQEVSENSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9025..9234)
                     /locus_tag="ACHKA1_001068"
     CDS             complement(9025..9234)
                     /locus_tag="ACHKA1_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678756.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGYQEELQKVRQEFAQRFLSK
                     GMSREDVAEVTTLPLTEIDKLINSN"
     gene            complement(9303..10175)
                     /gene="rpnC"
                     /locus_tag="ACHKA1_001069"
     CDS             complement(9303..10175)
                     /gene="rpnC"
                     /locus_tag="ACHKA1_001069"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO5678757.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEERGRQEERQEFVLRLLNKGMSREDVAE
                     MANLPLAEIDKLIN"
     gene            10449..10829
                     /gene="panD"
                     /locus_tag="ACHKA1_001070"
     CDS             10449..10829
                     /gene="panD"
                     /locus_tag="ACHKA1_001070"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO5678758.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10833..12062)
                     /gene="yadE"
                     /locus_tag="ACHKA1_001071"
     CDS             complement(10833..12062)
                     /gene="yadE"
                     /locus_tag="ACHKA1_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO5678759.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12126..12566)
                     /gene="yadI"
                     /locus_tag="ACHKA1_001072"
     CDS             complement(12126..12566)
                     /gene="yadI"
                     /locus_tag="ACHKA1_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO5678760.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12671..13441)
                     /gene="yadH"
                     /locus_tag="ACHKA1_001073"
     CDS             complement(12671..13441)
                     /gene="yadH"
                     /locus_tag="ACHKA1_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5678761.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13438..14364)
                     /gene="yadG"
                     /locus_tag="ACHKA1_001074"
     CDS             complement(13438..14364)
                     /gene="yadG"
                     /locus_tag="ACHKA1_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5678762.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14473..15135
                     /gene="can"
                     /locus_tag="ACHKA1_001075"
     CDS             14473..15135
                     /gene="can"
                     /locus_tag="ACHKA1_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO5678763.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15176..15712)
                     /gene="hpt"
                     /locus_tag="ACHKA1_001076"
     CDS             complement(15176..15712)
                     /gene="hpt"
                     /locus_tag="ACHKA1_001076"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO5678764.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15918..18308
                     /gene="gcd"
                     /locus_tag="ACHKA1_001077"
     CDS             15918..18308
                     /gene="gcd"
                     /locus_tag="ACHKA1_001077"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO5678765.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRYSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            18347..18445
                     /locus_tag="ACHKA1_001078"
                     /pseudo
     CDS             18347..18445
                     /locus_tag="ACHKA1_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18510..20060)
                     /gene="cueO"
                     /locus_tag="ACHKA1_001079"
     CDS             complement(18510..20060)
                     /gene="cueO"
                     /locus_tag="ACHKA1_001079"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO5678766.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20226..20573
                     /gene="yacC"
                     /locus_tag="ACHKA1_001080"
     CDS             20226..20573
                     /gene="yacC"
                     /locus_tag="ACHKA1_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO5678767.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20679..21545
                     /gene="speE"
                     /locus_tag="ACHKA1_001081"
     CDS             20679..21545
                     /gene="speE"
                     /locus_tag="ACHKA1_001081"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO5678768.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21561..22355
                     /gene="speD"
                     /locus_tag="ACHKA1_001082"
     CDS             21561..22355
                     /gene="speD"
                     /locus_tag="ACHKA1_001082"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO5678769.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22393..22755)
                     /gene="yacL"
                     /locus_tag="ACHKA1_001083"
     CDS             complement(22393..22755)
                     /gene="yacL"
                     /locus_tag="ACHKA1_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO5678770.1"
                     /translation="MDYEFLRDTTGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22930..25527)
                     /gene="acnB"
                     /locus_tag="ACHKA1_001084"
     CDS             complement(22930..25527)
                     /gene="acnB"
                     /locus_tag="ACHKA1_001084"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO5678771.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEKEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25882..27729
                     /gene="yacH"
                     /locus_tag="ACHKA1_001085"
     CDS             25882..27729
                     /gene="yacH"
                     /locus_tag="ACHKA1_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO5678772.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAILAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHDDDYHHHDGGHRDGNGWQHNGD
                     NINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSATQ
                     LPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFSRR
                     QPLTQQQKDAARQRYQSASPEPRQAVRERMQTNPQNQQRREAARERIQPASPEQRQAV
                     REKMQTNPQIQQRREAARERIQSASPEQRQVFKEKVQQSPLNQQQRDNARQRVQSASP
                     EQRQVFREKVKESHPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(27800..29224)
                     /gene="lpdA"
                     /locus_tag="ACHKA1_001086"
     CDS             complement(27800..29224)
                     /gene="lpdA"
                     /locus_tag="ACHKA1_001086"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO5678773.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29432..31324)
                     /gene="aceF"
                     /locus_tag="ACHKA1_001087"
     CDS             complement(29432..31324)
                     /gene="aceF"
                     /locus_tag="ACHKA1_001087"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO5678774.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNTGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31339..34002)
                     /gene="aceE"
                     /locus_tag="ACHKA1_001088"
     CDS             complement(31339..34002)
                     /gene="aceE"
                     /locus_tag="ACHKA1_001088"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO5678775.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34163..34927)
                     /gene="pdhR"
                     /locus_tag="ACHKA1_001089"
     CDS             complement(34163..34927)
                     /gene="pdhR"
                     /locus_tag="ACHKA1_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO5678776.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYARREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            35468..36841
                     /gene="aroP"
                     /locus_tag="ACHKA1_001090"
     CDS             35468..36841
                     /gene="aroP"
                     /locus_tag="ACHKA1_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO5678777.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(36884..37738)
                     /gene="ampE"
                     /locus_tag="ACHKA1_001091"
     CDS             complement(36884..37738)
                     /gene="ampE"
                     /locus_tag="ACHKA1_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO5678778.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTVASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(37735..38286)
                     /gene="ampD"
                     /locus_tag="ACHKA1_001092"
     CDS             complement(37735..38286)
                     /gene="ampD"
                     /locus_tag="ACHKA1_001092"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO5678779.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDISNNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            38374..39267
                     /gene="nadC"
                     /locus_tag="ACHKA1_001093"
     CDS             38374..39267
                     /gene="nadC"
                     /locus_tag="ACHKA1_001093"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO5678780.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            39470..39910
                     /gene="ppdD"
                     /locus_tag="ACHKA1_001094"
     CDS             39470..39910
                     /gene="ppdD"
                     /locus_tag="ACHKA1_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO5678781.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTIRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            39920..41305
                     /gene="gspE"
                     /locus_tag="ACHKA1_001095"
     CDS             39920..41305
                     /gene="gspE"
                     /locus_tag="ACHKA1_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO5678782.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            41295..42497
                     /gene="hofC"
                     /locus_tag="ACHKA1_001096"
     CDS             41295..42497
                     /gene="hofC"
                     /locus_tag="ACHKA1_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO5678783.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGIMLTIANKLLMRRPTWLIARQKLLLRFPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(42532..43575)
                     /gene="guaC"
                     /locus_tag="ACHKA1_001097"
     CDS             complement(42532..43575)
                     /gene="guaC"
                     /locus_tag="ACHKA1_001097"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO5678784.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(43731..43775)
                     /locus_tag="ACHKA1_001098"
     CDS             complement(43731..43775)
                     /locus_tag="ACHKA1_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678785.1"
                     /translation="MIIKTLKMSARKRN"
     gene            43800..44420
                     /gene="coaE"
                     /locus_tag="ACHKA1_001099"
     CDS             43800..44420
                     /gene="coaE"
                     /locus_tag="ACHKA1_001099"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO5678786.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            44420..45163
                     /gene="zapD"
                     /locus_tag="ACHKA1_001100"
     CDS             44420..45163
                     /gene="zapD"
                     /locus_tag="ACHKA1_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO5678787.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            45173..45370
                     /gene="yacG"
                     /locus_tag="ACHKA1_001101"
     CDS             45173..45370
                     /gene="yacG"
                     /locus_tag="ACHKA1_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO5678788.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(45586..45975)
                     /gene="mutT"
                     /locus_tag="ACHKA1_001102"
     CDS             complement(45586..45975)
                     /gene="mutT"
                     /locus_tag="ACHKA1_001102"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO5678789.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(46035..48740)
                     /gene="secA"
                     /locus_tag="ACHKA1_001103"
     CDS             complement(46035..48740)
                     /gene="secA"
                     /locus_tag="ACHKA1_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO5678790.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(48802..49314)
                     /gene="secM"
                     /locus_tag="ACHKA1_001104"
     CDS             complement(48802..49314)
                     /gene="secM"
                     /locus_tag="ACHKA1_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO5678791.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(49545..50462)
                     /gene="lpxC"
                     /locus_tag="ACHKA1_001105"
     CDS             complement(49545..50462)
                     /gene="lpxC"
                     /locus_tag="ACHKA1_001105"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO5678792.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(50563..51714)
                     /gene="ftsZ"
                     /locus_tag="ACHKA1_001106"
     CDS             complement(50563..51714)
                     /gene="ftsZ"
                     /locus_tag="ACHKA1_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO5678793.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(51775..53037)
                     /gene="ftsA"
                     /locus_tag="ACHKA1_001107"
     CDS             complement(51775..53037)
                     /gene="ftsA"
                     /locus_tag="ACHKA1_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO5678794.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(53034..53864)
                     /gene="ftsQ"
                     /locus_tag="ACHKA1_001108"
     CDS             complement(53034..53864)
                     /gene="ftsQ"
                     /locus_tag="ACHKA1_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO5678795.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(53866..54786)
                     /gene="ddlB"
                     /locus_tag="ACHKA1_001109"
     CDS             complement(53866..54786)
                     /gene="ddlB"
                     /locus_tag="ACHKA1_001109"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO5678796.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQADMSFSQLVVRILELAD"
     gene            complement(54779..56254)
                     /gene="murC"
                     /locus_tag="ACHKA1_001110"
     CDS             complement(54779..56254)
                     /gene="murC"
                     /locus_tag="ACHKA1_001110"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO5678797.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAKVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(56308..57375)
                     /gene="murG"
                     /locus_tag="ACHKA1_001111"
     CDS             complement(56308..57375)
                     /gene="murG"
                     /locus_tag="ACHKA1_001111"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO5678798.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(57372..58616)
                     /gene="ftsW"
                     /locus_tag="ACHKA1_001112"
     CDS             complement(57372..58616)
                     /gene="ftsW"
                     /locus_tag="ACHKA1_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO5678799.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(58616..59932)
                     /gene="murD"
                     /locus_tag="ACHKA1_001113"
     CDS             complement(58616..59932)
                     /gene="murD"
                     /locus_tag="ACHKA1_001113"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO5678800.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADGRCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKISGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGVQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(59935..61017)
                     /gene="mraY"
                     /locus_tag="ACHKA1_001114"
     CDS             complement(59935..61017)
                     /gene="mraY"
                     /locus_tag="ACHKA1_001114"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO5678801.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(61011..62369)
                     /gene="murF"
                     /locus_tag="ACHKA1_001115"
     CDS             complement(61011..62369)
                     /gene="murF"
                     /locus_tag="ACHKA1_001115"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO5678802.1"
                     /translation="MISVTLSQLTDILNGELQGADITFDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(62366..63853)
                     /gene="murE"
                     /locus_tag="ACHKA1_001116"
     CDS             complement(62366..63853)
                     /gene="murE"
                     /locus_tag="ACHKA1_001116"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO5678803.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     NDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(63840..65606)
                     /gene="ftsI"
                     /locus_tag="ACHKA1_001117"
     CDS             complement(63840..65606)
                     /gene="ftsI"
                     /locus_tag="ACHKA1_001117"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO5678804.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(65622..65987)
                     /gene="ftsL"
                     /locus_tag="ACHKA1_001118"
     CDS             complement(65622..65987)
                     /gene="ftsL"
                     /locus_tag="ACHKA1_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO5678805.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(65984..66925)
                     /gene="rsmH"
                     /locus_tag="ACHKA1_001119"
     CDS             complement(65984..66925)
                     /gene="rsmH"
                     /locus_tag="ACHKA1_001119"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO5678806.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(66927..67385)
                     /gene="mraZ"
                     /locus_tag="ACHKA1_001120"
     CDS             complement(66927..67385)
                     /gene="mraZ"
                     /locus_tag="ACHKA1_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO5678807.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(67987..68991)
                     /gene="cra"
                     /locus_tag="ACHKA1_001121"
     CDS             complement(67987..68991)
                     /gene="cra"
                     /locus_tag="ACHKA1_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO5678808.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(69073..69159)
                     /locus_tag="ACHKA1_001122"
     CDS             complement(69073..69159)
                     /locus_tag="ACHKA1_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO5678809.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(69171..69662)
                     /gene="ilvN"
                     /locus_tag="ACHKA1_001123"
     CDS             complement(69171..69662)
                     /gene="ilvN"
                     /locus_tag="ACHKA1_001123"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO5678810.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(69665..71389)
                     /gene="ilvI"
                     /locus_tag="ACHKA1_001124"
     CDS             complement(69665..71389)
                     /gene="ilvI"
                     /locus_tag="ACHKA1_001124"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO5678811.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRSNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(71707..72651)
                     /gene="leuO"
                     /locus_tag="ACHKA1_001125"
     CDS             complement(71707..72651)
                     /gene="leuO"
                     /locus_tag="ACHKA1_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO5678812.1"
                     /translation="MPEVQTDHPEMAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT        17799 a        19806 c        17796 g        17553 t
ORIGIN      
        1 ggcatttttt gtttaaacga cctaaaataa caacaattca atacgccaca taaatatatt
       61 cacacccgtg ataactcacc cctattacat cctccaaaaa ctttgagtaa tatccgctcg
      121 caatattaat ttcatataat ttttgtaagg atgcatgtaa tgaaaaaagc acttctcgca
      181 gccgctctgg ttatggcttc tggttccgcc ctggctgtag atggtggtca tatcgacttt
      241 aacggtatgg tacagtccgg tacctgtaaa gtgggtgtgg tagatactgg tatgcacagc
      301 gttaccactg atggcgtggt taccctggat actgcgaatg ttactgatac ttttgctgaa
      361 gttagcgcaa ctgctgtcgg tttactgccg aaagagttca tgatttctgt tgagtgtgat
      421 ccaggtgctc cgaagaatgc tgagttaact atgggttctg caagctacgc gaacaccagc
      481 ggtaccctga ataacaatat gaacatcact gttaacggta ttgaaccggc tcagaacgta
      541 aacattgcag ttcataacat gaaaaacaaa gctggcgctg ctgaaattaa gcaggtccat
      601 atgaacaact cttctgaagt tcaggaactg acattagacg cagaaggtaa aggccagtac
      661 gtatttaacg catcttacgt taaagcaccg aacagcccgg ctgtaactgc tggtcatgta
      721 accactaacg cgctgtacac cgttgcttat aagtaattga tggtgtgttg agcataaggt
      781 ttgccagcag gcaaacctta ttttcgcgaa tttaatcttt ctgcagacag caaggagtgc
      841 ttatgtttcc cttggttaaa aaaacggtta gtgcattatt tgtgagtact ctgttagcca
      901 gtgcaccagc attcgctgac attattattt ccggaacacg tatcatttat aacgctgata
      961 aaaaagatgt taacgttcgt ctggaaaata aagggaatcg ccccttactg attcagaact
     1021 ggttggatac tggcgacgac aatgccgatc ccagccagat caaagtgccg tttaccgcga
     1081 ccccaccagt ttcacgtgtg gagccaaaac gcggtcaaac cgtaaaagtg atgtataccg
     1141 gtgcaacagc cctgccgaaa gaccgtgaaa gcgtctactg gtttaacgtg ctggaagtgc
     1201 cgcctaagcc gaaagatgct gaggctgaca aaaacttgtt acagctggct ttccgcacac
     1261 gcatcaaact cttttatcgc ccgagtgggt tacagggaga accggctgaa gcaccggcga
     1321 aaatcacctg gaaattaaac aacgctcagt tacaggcaaa taacccaacg ccatattacg
     1381 tgtcctttaa tgaggtcaag ctggaaagcg gcggcaaaac ctacaacgtc aattccagca
     1441 tggtcgcgcc gttcagccag gcaagctttg gcgtaaccgg tctgccagga agcgtgtcat
     1501 ccggaaaagt ggtttttaaa gcgatcaacg actttggcgg caacattgat ggttccgcaa
     1561 cattctaaaa aataaacgga ttctttttaa catcgccagg aatgcaacct tgtaccagtt
     1621 tactcatcaa aaaagccgta tcccgaaaaa aacgctactt gcggcctgtt gcgccctttt
     1681 ttacagcggc aacagttctg cggcagacac ggtggaatat gacagttcct tcttaatggg
     1741 aactggcgca tcaacgattg atgttaaacg ttatgctcaa ggcaacccga caccgccggg
     1801 tctctataat gtccgcgtgt ttgtaaacgg tcaggcgacg tccagcttag atattccgtt
     1861 tgtggatatt ggcgaaaaca gcgcagcggc ctgtattacc cacaaaaacc tggcacaact
     1921 tcacattaag caacctgaac agcctgtcac tttactcgcc agagaaggtg aggaagagga
     1981 ttgtctggat ttggcaaagt catacgaaaa ggcggatgtg cgctttgacg gtagtgacca
     2041 gtttctcgat ctgacgatcc ctcaggccta tgttttgaaa agctatggcg gctacgttga
     2101 cccttcttta tgggaatcgg gaattaacgc tgccacactg gcatataccc tgaacgcgta
     2161 tcacacaagt tcagataacg acaatagtga cagcgtctat ggcgcattta actcgggtat
     2221 caatttagga gcctggcact tccgtgcgcg cggtaactat aactggacaa cagataacgg
     2281 cagcgatttc gatttccagg atcgttactt acagcgtgac attccggcaa tccgttcaca
     2341 gataattatg ggtgacgcct acaccaccgg tgaaacgttt gactctgtca acgtccgtgg
     2401 tgttcgcctg tacagcgata gccgtatgct accttcggcg ctggccagtt acgccccgac
     2461 catccgcggt gtagcaaact ccaacgccaa agttacggtg acgcagaacg gttataaaat
     2521 ctatgaaact accgttccgc ccggtgaatt tgttatagac gacattagcc cctccggctt
     2581 tggtagcgaa ctggtcgtaa ccattgaaga agcggatggt tccaaacgca cctttacgca
     2641 acccttctcg tcggttgtgc aaatgcaacg tcctggtgta ggtcgttggg atttcagtgc
     2701 cggtaaagtc attgatgata gcctgcgttc cgaacccaat atggggcaag cctcttatta
     2761 ctatggtctg aataacctct tcacgggtta taccggcatt cagttcaccg ataacaacta
     2821 tcttgccggg ctgttaggtg tgggtatcaa caccagcatc ggcgcctttg cggtagacgt
     2881 tacccattcc cgtgctgaaa ttccggatga taaaacctac caggggcaaa gttatcgcgt
     2941 gacctggaac aaacttttcc aggataccgg gacatcattt aacctcgcgg cgtaccgcta
     3001 ttccacccag gattacctgg gcctgcatga tgcgttagtc ctcattgacg acgccaaaca
     3061 tttgtctgcc gatgaagaca aaaacaccat gcagacgtac tcacgtatga aaaaccagtt
     3121 taccgtcagc atcaaccagc cgctgaaaac gacttacgaa gattacggtt cactgtttat
     3181 ttccggtagc tggacggatt actgggcggc gaacaatagc cgcactgaat ataatgttgg
     3241 ttacagtaaa agcgtttcgt ggggcagttt cagcgtcaac ctgcaacgta gctggaatga
     3301 agacggcgag aaagatgacg cgatgtacgt cagcgttagc gtacctattg agaatatttt
     3361 aggtggcaag cgcaaatctt ctggtttccg caatttaaat actcagctca ataccgattt
     3421 cgatggttca catcagttga atgttaacag ttccggtaac actgaaaaca atctggtgaa
     3481 ctacagtgtc aacgctggtt atagcctcga taaaaactcc ggcgatttag cctctgttgg
     3541 cggttatctc aactatgagt ctgggttagg cggtatttcc gcttcggcct cggccacttc
     3601 tgataatagc cagcagtact ccatctccac cgatggcggc tttgtattac acagtggtgg
     3661 tttaacgttc actaacaaca gtttcagcag taacgatacg ctggtgttaa tcaacgcccc
     3721 aggtgctaaa ggcgcacgaa tcaataacag taataacgaa atcgatcgct ggggatatgc
     3781 cgtgacgtcc tctgtcagcc cctaccgtga aaaccgggta ggtctgaaca ttgaaacact
     3841 ggaaaacgat gttgaactga aaagtaccag cgcaaccacc gtaccacgca gcggctccgt
     3901 tgttttgacc cgtttcgaaa ctgacgaggg gcgttctgcc gtgctgaata ttactgccgc
     3961 caatggcaaa tccattccgt ttgctgcgga ggtttaccag ggtgaggtga tgatcggcag
     4021 catgggccag ggtggtcagg catttgtgcg cggtattaac gacagcgggg aattaacagt
     4081 gcgctggtat gaaaacaacc aaaccgttga ctgtaagttg cactaccagt tcccggcgca
     4141 gccacaaacg ctgggaagta ccaacacctt attacttaac aatcttacct gtcaggtagc
     4201 aaatcactaa tatggaaaat tctatgaaaa ggatactact aacatccgcg ttaataggtc
     4261 tgggtttacc ggcggttggc tcagcaacag atcttaatgt caattttacg gccaccgtat
     4321 tagccaccac ctgtacgata actatcgtac aagatggtgg tccagtagtg acgggtagca
     4381 acggtgaata tagcctgaca attcccgatg ttggcctgga taaggttgtc actggagatg
     4441 ttgcagctca agccaacttt aaattagtgg cttccgattg cagcaatggt tattcaaaga
     4501 tttttaccac acttacagga acaacgactt ccggtaagtt gattgtcaac gaagcaagct
     4561 ctggcgctac gggtgtgggt atggggatta agcgtagaga taccgcagac tcgactttct
     4621 ttacacccaa taatactgac aaatttgaat ggaccgccga tgagaaaact aacggtgtgc
     4681 ctctgaccgt agcactgcgc gaaacaacgg cgggagcagg acgcaccggt gcgtttcagg
     4741 caaaagccac ctttaacttc acctacgaat aacagggaca gccaccatga aattaaaagt
     4801 catcgctaca ctgattgcta ctgttgccgt gggtgtcagc gttagcaaca atctcactta
     4861 tgcgagtacg acatccgcct ctttaaccgt aaacagtaac ctgactatgg gtacctgtag
     4921 tgcgaaggta gtggatacca gtggtgccgt gatcgacacc gttgtcttgg gcaatgttta
     4981 tatttctgaa ctggtagcca aaagcaaggt acaaccattt aaaatccgct tcagcgattg
     5041 tgccggcctt ccaggaaaga gtgctcaact cgtcttagcg cccagagcaa atgtcggttg
     5101 tgcaggcggc atgtctaaca ctgcagcctt ttcaaatcaa ctcaactcca ccaccgatgg
     5161 tggttcaagc agagcagcag tcgaagtatg gacgactaca agccccgagg gaaatggcag
     5221 tgagcaattc aattgttatc agcgaaacgt catcaatgtc gatctttcta atgccagcac
     5281 aacacagcct tatgattttg atttaagcgc acgaatgatt atcgctgaag gatggtcggt
     5341 caatgacgta ttaccgggta gattcctctc tcctacgact ttcacaatca cttatcagta
     5401 atagcggata aggatattag aatgctatgc aggcatcata aatttgtacg ttttttgggg
     5461 ctggcaacag ccctcattac gccttttgcc tatgctggcc aggatgtgga tctcacggcg
     5521 cagattgtgg ccagcacctg tcaggttgaa gtcagtaata atggcgttgt cgatctcggc
     5581 acagtgacgc tggactattt tgccgacaat gtaaccccga caacggatta tgcgggtggc
     5641 aaaacgttta atgtgaatgt ggtctcttgc gataacattc aaacgacaca gtcgcaaatg
     5701 aaattggatt ttcagccgca ggcgggatcg cttgcacagg tgaataatca aatttttagt
     5761 aatgaatacg aacagcaggc taccggggcg aaaaacgtcg gcattgtgat attttcggcc
     5821 caaccgaatc agcagacatt taacgtgcga ggaacggatg gttcctcgac agcaatttat
     5881 tcggtcgcgc ctggcaatgc tgttccctca acctggacct tttattcccg tatgcagcgg
     5941 gtgaataatg cccttcctcc tgaatcggga atggtgagaa gccaggttat tgttaacgta
     6001 tcttacgaat aataaggata attatgcaaa ggaaaggcaa taaactgttg attcagttat
     6061 gcagtgcgat actgctattt tttaccacat cctggtatgc attggcgaat gaatgttata
     6121 tagagagaaa tgctgaaggg ccttatgata tggtgataaa cccaaagccc ttcagtatac
     6181 agtcagcgat ggtcacagtc ccggaagaaa taggcttcgc gacctgggat tcttatattg
     6241 atcttcgggg cgataagatt ggttgtaaat ccttaggtag taacacaagc tcagtgcatt
     6301 ttcttaatac tgctgatgcc agtctcttat caacctatac taccagccat ggcgatgctt
     6361 tattgaaaac aaccgttccc ggtattgttt attcagtaga attagtatgt acaagttgtg
     6421 gagccgcgga ggagcttgat ttgtatcttc ctgcacaaag tggtgcagat aatcatacag
     6481 ataacgccag ctcaaagtgg gcctatgagt atagtgacga gagctggtat ttacgatttc
     6541 gttttttctg gaccccggaa tttaaacctc aaaatggggt ttcagaagga actgcgatac
     6601 caggtaaaat agcttcctgg tatataggca ataatgacca accatggata aattttttcg
     6661 ttgatacaag ctccgttcat ttttacgttg acgaaccgac ctgtgcaaca gttgccctcg
     6721 cacaagatca gggcaacgtc agtggcaacc aggtaacgct tgggaacagc tatgtttcgg
     6781 aagtgaaaaa tgggcttacg cgggaaatcc ctttttctat ccgtgctgaa tactgctatg
     6841 ccagtaaaat tacggttaag ttgaaagcgg caaataaacc cagcgacgcc acactagtag
     6901 gtaaaacgaa tggctcggct tcaggcgtgg ctgtaaaagt aaattcaact tatgacaata
     6961 gcaaagtatt gttaaaagca gatggtagca acacggttga ctataacttc gccgtctggt
     7021 caaacaacct gctgctttta ccttttacgg cgcagctggt accggatggc agtggtgctg
     7081 ctgtcggtgt tggaaccttc tctggtaacg cgaccttctc ctttacctac gaataagtaa
     7141 aacgtcattc cttcctcatc gcaccagagt tctgctctgg tgcgaacgtt ttccccacac
     7201 taaaaaccat tgcccccctg atttcgactg ttagaatgcg atcaatctga ctttagccat
     7261 cagggtacgt tatgaaaccg accaccattg cttcattgca aaaatgcaaa caggaaaaaa
     7321 aacgtttcgc gaccatcacc gcttacgact atagcttcgc caaactcttt gctgacgaag
     7381 ggctgaacgt gatgctggtg ggtgattcgc tgggcatgac cgttcagggc cacgactcca
     7441 ctctgccagt aaccgttgcc gatatcgcct accacactgc cgccgtacgt cgcggcgcgc
     7501 caaactgcct gctgttggct gacctgccgt ttatggcgta tgccacgccg gaacaagcct
     7561 ttgaaaacgc cgcaacggtt atgcgtgccg gtgctaacat ggtcaaaatt gaaggcggtg
     7621 agtggctggt cgaaaccgta aaaatgctga ccgaacgtgc cgttcctgta tgtggtcact
     7681 taggtttaac cccacagtca gtgaatattt tcggtggcta caaagttcag gggcgcggcg
     7741 atgaagcgag cgatcgactg cttagcgatg cattagcctt agaagccgct ggtgcacagt
     7801 tgctggtgct ggaatgcgtg ccggttgaac tggcaaaacg tattaccgaa gcactggtga
     7861 tcccggttat tggcattggc gcaggcaacg tcactgacgg gcagatcctc gtgatgcacg
     7921 atgcgttcgg cattaccggc ggccatatcc ctaaatttgc caaaaatttc ctcgctgaaa
     7981 cgggcgacat ccgcgcggct gtgcggcagt atatggctga agtggagtcc ggcgtttatc
     8041 cgggcgaaga acacagtttc cattaaggag tgacgttgtg ttaattatcg aaaccctgcc
     8101 actgctgcgc cagcaaattc gccgcctgcg tatggaaggc aagcgcgtgg cgctggtacc
     8161 taccatgggc aacctgcatg atggccacat gaagctggtc gacgaagcca aagcccgcgc
     8221 cgatgtggtc gtcgtcagta ttttcgttaa cccgatgcag ttcgaccgcc cggaggacct
     8281 ggctcgttac ccacgcacct tgcaggaaga ctgcgaaaag ctgaacaagc gcaaagtgga
     8341 tctggttttc gcgccctcgg taaaagagat ctacccgaac ggtactgaaa cccatactta
     8401 cgttgacgtt ccagggcttt cgaccatgct ggaaggcgcc agccgtccgg ggcattttcg
     8461 cggcgtgtcg actatcgtca gcaagctgtt taacctggtc cagccggaca tcgcctgctt
     8521 cggtgaaaag gactttcagc aactggcgct gatccgcaaa atggttgctg atatgggctt
     8581 tgatattgag attgtcggtg tgccaattat gcgcgccaaa gacggtctgg cactgagttc
     8641 ccgtaacggt tatctgacgg cagaacaacg caaaattgcg cccggtctgt acaaagtttt
     8701 aagttcgatt gccgacaaat tgcaggctgg cgaacgggat ctcgatgaaa ttattgctat
     8761 tgccgggcaa gaactgaatg aaaaaggctt ccgcgccgat gatattcaga ttcgcgatgc
     8821 cgacacgttg caggaggtct ctgaaaacag caaacgggcc gtaattctgg tagccgcctg
     8881 gcttggcgat gctcgcctga tcgacaacaa gatggtcgaa ctggcgtaat acttaactgg
     8941 cgctacggct gatggcgcca gttattaatt tgccctacgt catggcgatg acgtctgtaa
     9001 gtgattattc atatcgctgg caatttagtt agagttaatt agcttatcaa tctctgtaag
     9061 aggtaaagtt gtcacctctg caacgtcttc ccgagacatc cctttactta gaaaacgctg
     9121 ggcgaattcc tgtcttacct tctggagttc ctcttgatat cccttctgaa atcccttctc
     9181 ttcaaaccac tgcgccaacg tctgcctggt ttctctccgt gtctttattt ccataggttc
     9241 ccttaattat ctggtcagtt aacaataaca attatctggc tataactgat gatgcagaca
     9301 acttagttaa tcagcttatc aatctcggca aggggtaaat ttgccatctc tgcaacgtct
     9361 tcccgagaca ttcctttatt cagaagacgc aggacgaatt cttgcctttc ctcctgtctt
     9421 cccctctctt caaaccactg cgccagcgtc atcatagact cccctcccgt ttccctgtct
     9481 ctcagcacac cataaaacaa atccgcttgt tcagtatgac cgcgttgcag catatagttt
     9541 tgcatggcaa ctaactgact tccgctagtg tacccttcgt caatcagcgt gaccagttgc
     9601 tccagcaata acattaagtc gcgctggcga atatgttttt gcagtagttc gagaatcgca
     9661 atccgccgat gttgcatgat ttcgtcatcc ggtgtgatgg tgatatccac cagcgggaaa
     9721 ggactgttat agacgcgtcg cgccagctca ggcgagtaaa acatatcaaa ccagcacatt
     9781 gagagcggat aaggtgtggc ctcgccctga taaaacagta tcggcaccac cagcggcagc
     9841 ttatcgtggt cagcctccag atgccggtgc atggcggcaa tagaataacg catcatgcga
     9901 aaagccattt tcttatctgg cttgctttga tgttcaatca ctacatgcag ataaccggga
     9961 ttgccctgca tttgcacgga atagagcacg tccgtgctgt gtcctttcag gctctcttca
    10021 atgaaactcc ccgactctaa atgaagcgtg ttgaggtcac aaagttctcg taattccact
    10081 ggcaaatgta tctccagaaa gtcgcgagcc gtctccgcat gcattaaaaa ttgtttaaat
    10141 accgcgtcat gcggtgtggt actcggtgca tccatcttcc ctgtcttccg tgttgtcttg
    10201 cgtgaaggca gtatccagtc aaggcgtgct atcgtcagcc acctctttag tgtttaccga
    10261 gcagcgttca gagaaatttc aattggttac agccgttaca tggcacgctt cggcgcggta
    10321 cgcaaactgc tgatgcaaat gccgtcagat gagtagacac taaacaaaaa tcgggcaata
    10381 ctgcgtgaga atttcctcaa agcaggcacc gcctgcttcg ttaacgacag ggtagaaagg
    10441 tagaagttat gattcgcacg atgctgcagg gcaaactcca ccgcgtgaaa gtgactcatg
    10501 cggacctgca ctatgaaggt tcttgcgcca ttgaccagga ttttcttgac gcagccggta
    10561 ttctcgaaaa cgaagccatt gatatctgga atgtcaccaa cggcaagcgt ttctccactt
    10621 atgccatcgc ggcagaacgc ggttcgagaa ttatttctgt taacggtgcg gcggcccact
    10681 gcgccagtgt cggcgatatt gtcatcatcg ccagcttcgt taccatgcca gatgaagaag
    10741 ctcgcacctg gcgacccaac gtcgcctatt ttgaaggcga caatgaaatg aaacgtaccg
    10801 cgaaagcgat tccggtacag gttgcttgat tgttatccct gcggctggtt actcaccagc
    10861 cgcgacatcg tctccagcga atccgttctt aagatataaa gtcgttttag taacaacgga
    10921 ttatcccccg gttttacttt gcctttcatg gttgtcaccg ccaggtgaaa tccggcatcg
    10981 tttgctgcct tcacggcttt gtcattaaag ccgccaaacg gatacgaaag ataccagaca
    11041 tgcggattaa attgcgccag agcgcggcgt gaacgtgcaa aatcaaacag aatattgtgc
    11101 tcactacggc tcagtaatat gggtcggcga taaccatcta cccgatgcaa aaaatgggta
    11161 tgtgactgga aatcaaatac atcgcgaatt tcgttaagct cagaaacgct cataaattgc
    11221 agcgattttg ggttccactt ctgcgggtga cgtttgatgc gtgaggtaac aataaacgcc
    11281 gtcgccttca tgccatattg tttcaacaca ggatacgcat agcggctcac cgacttgagg
    11341 ccatcatcaa aggtaatcac caccgctcgc gcagggagat tgatcttatt cttcacgtaa
    11401 ccttccagct gcaccatgct cagtgtcgcg tatcccctgt cgcgcagcca ggccatctgg
    11461 ttattgaaag cgcgtaccga ggtggtcgtc gaagtatggc gaaaacgggt gttttcttcg
    11521 tcgcgcagaa tatggtgata agttagcacc ggaaggccat tatcgggttg ggcatccagc
    11581 gcgctgatat aggccagtcg atcgccaata cggacctgat accaggtttg atttaacctg
    11641 tctttcagtt tatgcaaaat cgggtagcgc aaattgtccg ccagtacccc aaatggcgca
    11701 ctgcccgcac tcggcgcgtt atagaccggc gtatctttcc aggtaactaa gttctgatta
    11761 ctcagcggct tgttgaggtc acccaaaccg tcttcaactt tttgtcgccc ctgaaccggc
    11821 tcgagatgcc ctttatcgat aaacccttta ccaaagccaa aattaaatgc gtaataactt
    11881 gcggcagtgg gctccacggc aatgatttgt ccggcccgaa tattccccac ggtcaccatc
    11941 ttgtcaccaa tttgcgccca gaccgcagca ttttcgatgg tttgcatata acgggcaggt
    12001 aacgcggcac tgacacttgc ggtaaacagc atcagcagga gaataacagc ttgtttgtac
    12061 atgaaaaaat aaccgagggt gaaacaacgc ttttattgta gcaaaagcgc tataagaatc
    12121 gtgggttaat actcatacaa attatgtttg aggacgaaag gcggattttt ttgttgttgg
    12181 tgccaaagac tactcacctc cggcgcaccc agttcgacaa tacgctcgcg aaactctgaa
    12241 ctggtcatgg tttcacggca agccatcatc tgttcaatta acggtaacgt gacaccagaa
    12301 atcacttcgc aacgggagtg tttgtggctt aataatgaag ccacgcgata cggtggcgct
    12361 ccggctatat cggttaagaa gatgacaccc tcaccagagt ccgcttcatg cagagcatcg
    12421 cacatcatgc ggctgagcat attagagctt aacccacgcc agaaattcac ggcccggcac
    12481 tgaagaagcg ccccatgttt tttctccagc gcatccagta tctcttgcgc ccggtcatcg
    12541 tgacaggtaa ttacccaacc taacatcgcc ttcctcctta gccgaaaatc agtctaccct
    12601 gaccattgtt gataaagggt gatagccgtc aaaactcctc tcccccaaat ccagaggaga
    12661 ggaaatagcc ttagctacgc aaaccacgtc cacgttggat cagcgaccaa cagatcaaat
    12721 aaaacgccac aataaagacc accagtacgc caaaggtagt gaccagcgga acatcattga
    12781 taccgaggaa gccgtagcgg aaaccactga tcatataaac gattgggttc aggtgcgaca
    12841 gcccttgcca gaacggcggc aacaaagtca gggagtaaaa gaccccaccc aaatacgtga
    12901 gtggcgttaa cacaaaggtt ggcaccaggc tgatgtcatc gaacgttttg gcaaacacac
    12961 cgttcagcaa acccgcaagg gagaacaaca ccgccgtgag caccagggtt aaggcaacga
    13021 atacccacga atgcacctga aacggcacaa aaaacagtga aattgccgtc accagaatgc
    13081 caacaaacag accacgcgcc acgccaccgc cgacatatcc ggcaataatg acgtgagtcg
    13141 gaaccggcgc taccagcagc tcttcaatat tacgctggaa tttggcacca aaaaatgatg
    13201 acgcgacgtt ggcgtaggcg ttggtgatca ccgacatcat gatcagcccc ggtacgatga
    13261 actgcatgta gctgaagcca tgcatatcgc caatacgcga accaatcagg ttaccgaaaa
    13321 taataaagta aagggtcatg gtgataactg gcggcaccag cgtctgcacc cagatacgca
    13381 taaagcgatg gatctctttc gcccagatgc tttttaacgc cacccagtaa agatgcatca
    13441 tgcgcgatct ccttgttttt cattaaccag tgaaacaaac agctcttcca gacggttagc
    13501 tttgttacgc atacttaata cctgaatgcc ctgctcactt aactgcgtaa atacgctgtt
    13561 gatcccctgc tcacgcagca cttcaacttc cagcgtcgcg gtatcgacca gtcgatactg
    13621 atagccatcg agcttcggta acggactttt cggtgcgaga tcgagaataa aggtttccga
    13681 tttcagcttc gccagcagcg ccttcatcga ggtattttcc accagctcac cgtgttgaat
    13741 aatgccgata ttgcggcaca gcatttctgc ttcttccagg tagtgtgtgg tgagaatgat
    13801 ggtggtgcct ttgtcgttta aatccttcaa aaagccccac attgagcggc gaagttcaat
    13861 atccacgcct gcagtcggct cgtcgagaat cagtagttta ggttcatgca ttaacgcacg
    13921 ggcaatcatt aaacggcgct tcatcccgcc agataacatg cgcgcacgtt cgttgcgttt
    13981 tccccataaa tcgagctgct taagatattt ttcgctgcgg atgtacgcct ctttgcgctc
    14041 cacaccgtag taccctgcct gattcaccac aatttgctgc acggtttcaa acgggttgaa
    14101 gttaaattcc tgcggcacca gtcccaactg acgtttagcg ttcacgacat ctttctcgag
    14161 atcgtaacca aatacgctga cccgcccgga ggttttattt accagagagc tgataatacc
    14221 gatagtggtc gatttcccgg ccccgttcgg cccgagaagc gcataaaaat cacccgcttc
    14281 gacctgcaaa tctattccac gaagcgcctg aacgccgcct ggataggttt ttttaagctg
    14341 ttgaagttcc agtgcaatgg tcataaattt ttacttacct tacgttctta cactttatct
    14401 gtggtttaaa tcgtcccgga gttgccctat attagccaaa cgtaattatt tggttacagg
    14461 tcgttaacct ccatgaaaga catagataca ctcatcagca acaatgcact atggtcaaaa
    14521 atgctggtgg aagaggatcc cgggtttttt gagaaactgg cacaagcgca aaaaccgcgc
    14581 tttctatgga ttggatgttc cgacagtcgc gttcctgcag aacgtttaac cggtcttgag
    14641 ccgggcgaac tctttgttca ccgtaatgtt gctaacctgg tcattcacac cgacctgaac
    14701 tgcctttccg tggttcagta tgcagtggat gtactcgaag ttgaacacat tattatctgt
    14761 ggccactacg gttgcggcgg cgtacaagcc gcagttgaaa acccggaact ggggcttatc
    14821 aacaactggc tgctgcatat ccgcgatatc tggttcaaac atagctcatt gctcggcgaa
    14881 atgccgcaag agcgccgtct ggataccttg tgtgaactga acgtcatgga acaggtgtat
    14941 aacctgggcc actccaccat tatgcaatca gcgtggaaac gcgggcagaa agttaccatt
    15001 cacggctggg cctatggcat tcacgacggc ttgctgcgtg atctggatgt tactgccacc
    15061 aaccgcgaaa cccttgagca acgttaccgt cacgggattt ccaacctcaa gctgaaacac
    15121 gccaaccaca aataaaaatg ccatgccgga tgcaacacat ccggcaactt cacacttact
    15181 cgtccagcag aatcactttg ccgatatacg gcagatgacg ataacgctgt gcgtaatcaa
    15241 taccgtaacc caccacaaac tcatccggga tcgagaaacc gataaattct accgggacgt
    15301 tcacttcacg gcgggacggt ttatccagca gcgtacaaat cgccagcgac ttcggttcgc
    15361 gcaggcttaa gatctcacgc actttcgaca gtgtattccc cgagtcgatg atatcttcaa
    15421 caatcagcac gtccttgcca cggatatctt catccagatc tttgaggatt ttcacatcac
    15481 gggtggtgga catgccgcta ccgtagctgg aggcggtcat aaagtcgact tcatgagata
    15541 cctgaacttc acggcacagg tccgccataa acataaatga gccacgcagc agacccacca
    15601 gcaccatatc gctgccgctg tctttgtaac gctcagtaat ctgacgaccc agttcggcga
    15661 tacgcgcttt aatctccgct tcggggatca ttacttctac agtatgtttc atatctctaa
    15721 ccatatgatt taaaaacaaa tcacttcgca cgggcaggtt ttggcaccgc aacaaagcgc
    15781 aagacacgca gtataccagt aaaactattc attcgcggaa tctgttaata aagctcgaat
    15841 tgtgatgacg gtcacacatg ttaaaaccta taattaattg ctactaaaat attaatgaat
    15901 tgaaatggtg tctctttatg gcaattaaca atacaggctc gcgacgatta ctcgtcacgc
    15961 taacagccct ttttgcagcg ctttgcgggc tgtatctact cattggcgga ggctggctgg
    16021 tcgcgattgg cggctcctgg tactacccta tcgctggcct tgtgatgctc ggcgtcgcct
    16081 ggatgctgtg gcgcagtaaa cgtgccgcgc tttggctata cgccgccctg ctgctcggca
    16141 ccatgatttg gggcgtctgg gaagttggtt tcgacttctg ggcgctgact ccgcgcagcg
    16201 atattctggt cttcttcggc atctggctga tcctgccgtt tgtctggcgt cgcctggtca
    16261 ttcctgccag cggcgcagtt gctgcgctgg tggtcgcact gctgattagc ggtggtatcc
    16321 tgacctgggc cggatttaac gatccgcagg aaatcaacgg caccttaagc gccgatgcca
    16381 cacctgccga agctatctca cccgttgccg atcaggactg gcctgcgtat ggtcgtaatc
    16441 aggaaggtca acgctattcg cccctgaaac agatcaacgc cgataacgtc cataacctga
    16501 aagaagcctg ggtgttccgt actggcgatg tgaagcagcc gaacgatccg ggtgaaatca
    16561 ccaatgaagt gacgccgatt aaagtgggcg acacccttta cctgtgtacc gctcaccagc
    16621 gactgtttgc gcttgatgcc gccagcggca aagagaaatg gcattacgat cctgagctga
    16681 aaaccaacga gtctttccag cacgtaacct gccgtggcgt ctcttatcat gaagccaaag
    16741 cagaaacggc ttcgccggaa gtgatggcgg attgcccgcg tcgtatcatt cttccggtca
    16801 atgatggtcg actgattgcg attaacgctg aaaacggcaa actgtgcgaa accttcgcca
    16861 ataaaggcgt gctcaatctg caaagcaata tgccagacac caaaccgggt ctgtatgaac
    16921 cgacttcgcc accgattatc accgataaaa ccattgtgat ggcaggttca gttaccgata
    16981 acttctcaac ccgcgaaacg tctggcgtga tccgtggttt tgatgtcaac accggggagc
    17041 tgctgtgggc ttttgatccg ggcgcgaaag atccgaacgc aatcccgtct gacgaacaca
    17101 cctttacctt taactcgcca aactcgtggg caccagcggc ctatgacgcg aagctggatc
    17161 tggtgtatct gccgatgggc gtgaccacgc cggatatctg gggcggtaac cgcacaccgg
    17221 aacaggaacg ttatgccagc tcgattctgg cgctgaatgc cacaaccggg aaattggcgt
    17281 ggagctacca gaccgttcac cacgacctgt gggatatgga tcttccggca cagccgacgc
    17341 tggcggacat caccgttaat ggtcagaaag tgccagttat ttacgctccg gcgaaaaccg
    17401 gcaacatttt tgtgctcgat cgtcgtaatg gcgaactggt ggttccggca ccggaaaaac
    17461 cggttcccca aggtgctgcc aaaggcgatt acgttacccc tactcaacca ttctcggaac
    17521 tgagcttccg tccgacgaaa gatttgagcg gtgcggatat gtggggagcc accatgtttg
    17581 accaactggt gtgccgcgtg atgttccacc agatgcgcta tgaaggcatt ttcacgccgc
    17641 catctgaaca gggcacgctg gtcttcccgg gtaacctggg aatgtttgaa tggggcggga
    17701 tttccgttga tccaaatcgt gaagtggcaa ttgccaaccc aatggcactg ccgtttgttt
    17761 cgaaactgat cccacgcggt ccgggtaacc cgatggagca gccgaaagat gccaaaggca
    17821 cgggtacgga atccggcatt cagccacagt acggtgtacc gtatggtgtc acgctcaacc
    17881 cgttcctctc accatttggt ctgccatgta aacagccagc atggggttat atctcggcgc
    17941 tggatctgaa aaccaatgaa gtggtgtgga agaaacgtat tggtacgccg caggacagta
    18001 tgccgttccc gatgcctgta ccggtgccgt tcaatatggg tatgccgatg ctgggcgggc
    18061 caatctccac ggcgggtaac gtgctgttta tcgccgctac ggcagataac tacctgcgcg
    18121 cttacaacat gagcaacggt gaaaaactgt ggcagggtcg tttaccagcg ggtggtcagg
    18181 cgacgccaat gacctatgaa gtgaatggta agcagtatgt ggtgatctcc gcaggcggtc
    18241 acggttcatt tggtacgaag atgggcgact atattgtggc ttatgccctg ccggatgatg
    18301 tgaagtaaga cttgctcaga ttgctgacaa cgtgcgcgtt gttcatgccg gatgcggcgt
    18361 gaacgcctta tctagcctac aaaattgtgc aaaatcaatg gattgcacag gtagcgtagg
    18421 cctgataagc gtagcgcatc aggcagattt gcgtttgtca tcagtttaat gcccggagag
    18481 atccgggcat attcccgaat acggtctttt tataccgtaa accctaacat catccccgta
    18541 tcttcatgct ccagcagatg gcagtgcgcc atataagcat gttctttcgg tgcgtcgtga
    18601 ttaaacttca ccagcacttc actgacattg ccttctacct taacggtatc tttccagccc
    18661 gcgcgatgag ccgctggcgg tttgccattt tctgacaaga tacggaactg cgtgccgtga
    18721 atatggaacg gatgcagcat catgtcgccc acgccagaga taacccaacg ttcgtactgc
    18781 cctttcgccg ccgcaaacat cggcttattc atatcaaacg cctgaccgtt gattttattg
    18841 gcatggtgga aatcgaactt cccgccgtgg ttcatatgat tcatattgcc gtgccccata
    18901 tggcccatca tctggctgtg atccattccg gccatcgcct gatcgccata tttctccatt
    18961 agcatctgca tccccatcat atcgagcatc gggtccatag aaagttgcag cttgcgtacc
    19021 gtcagccctt ccagcgaagg taacgcaggc aggctactta atgtgtctgg caaagcaccg
    19081 gaggcactaa tagcaatcgg ctgaatccgc attaccggat gcggcttgtc aaacggtgca
    19141 atcgccatcc ccatctggct gaccggcagc gtcaccaggt caaagggttt gttgtcgtta
    19201 acctccacca gcacttcaaa acgctcgccc atcagcaccg gcagctcgtt caccttcacc
    19261 ggttcaggta gcagaccacc gtcgctggca atcacataaa gcgggcgatt gtcgctggtg
    19321 gcgaaattga gcgagcgggc attacagcca ttgagcaaac gcaggcgcag ccaaccacgc
    19381 ggggcagcgt gttgcgggta gattgcaccg ttggtcagca acgtatcgcc aaaccagccc
    19441 acggcggcgg tcatcacatc cagttgataa tcaatctgcc cgtcggcgct aaatttctta
    19501 tcctgaacga tcaccggaac atcatcgata ccccactgtt ttggcagcat taatttcagg
    19561 atctcgtcat cttcaatcac caccagacca gccagcccca tcgccacctg tcgcccggtc
    19621 ttgccatgtt gatgtggatg gaaccagcag gtagcggcag gttgatcaac gttcaacgtc
    19681 accgagcgct tgccacctgg cggaataatt ccctgtgggc cgccgtcgac ttcacccggt
    19741 acttccagcc cgtgccagtg caacgtcgtc tcttccgtca gttggttata gatatcaacc
    19801 gttaccgctt tgccgcgctg taatttcacc gccggcccca gcagattgcc gttatagccc
    19861 caggtagttg cagttttctc gccaaaggta gactgacctg cgccaatagt taactgaatg
    19921 cgattacgag catcggtcgt gagcaaatca gggatcggta acgttgggcg ttccgccgca
    19981 aagactgcac ggctccacag tggcaaggct gaagccacac ccagtgcgac ggaatatttc
    20041 aagaaatcac gacgttgcat agttatttcc ttattcttaa gcaggcgaaa caaaatcaaa
    20101 cgttgagcat agtccttccc cttacgggaa ggtcaagccg cagacacaat ttaatcgaca
    20161 ataatggtcg tcgcctcgct cgcagtgtgc taacgtttat cttctttaag cccctggtag
    20221 aagcaatgaa gacgtttttc agaacagttt tattcggcag cctgatggcc gtttgcgcaa
    20281 acagttacgc gctcagcgag tctgaagccg aagatatggc cgatttaacg gcagtttttg
    20341 tctttctgaa gaacgattgt ggttaccaga acttacctaa cgggcaaatt cgtcgcgcac
    20401 tggtcttttt cgcccagcaa aaccagtggg atctcagtaa ttacgacacc ttcgacatga
    20461 aagccctcgg tgaagacagc taccgcgatc tcagcggcat tggcattccc gtcgctaaaa
    20521 agtgcaaagc cctggctcgc gattccttaa gcctgcttgc ctacgtcaaa taatccctga
    20581 tacctttttg tagaaatatt gaccgtgcat ccgcggtcaa tgttagctat tatgttgcgc
    20641 ccttttttta cgggtgttaa caaaggaggt atcaacccat ggccgaaaaa aaacagtggc
    20701 atgaaacgct acacgaccag tttgggcagt actttgcggt agataacgtt ctgtatcatg
    20761 aaaagaccga tcaccaggat ctgatcattt ttgagaacgc tgcatttggt cgcgtaatgg
    20821 cgctggatgg cgtagtacaa accaccgagc gcgacgagtt tatctatcat gagatgatga
    20881 cccatgttcc gctactggcc cacggtcacg cgaaacatgt gctgattatc ggcggcggcg
    20941 acggtgccat gctgcgtgaa gtaacccgac ataaaaacgt tgagtcaatc acgatggtgg
    21001 aaatcgatgc gggtgtcgta tcgttctgcc gtcagtatct acccaaccat aacgccggta
    21061 gctacgacga tccgcgcttt aagctggtga tcgatgatgg cgtcaatttc gttaatcaaa
    21121 ccagccagac ctttgatgtc attatctccg actgcaccga tcctatcggt cccggcgaaa
    21181 gccttttcac ttcggcattt tatgaaggct gcaaacgttg cctgaatcct ggcggtatct
    21241 tcgtcgcaca aaacggcgtc tgctttttac agcaggaaga agccatcgac agccatcgca
    21301 aactcagcca ttacttcagc gacgttggct tttatcaggc ggcgatcccg acctattacg
    21361 gcggtatcat gacttttgca tgggcgacag ataacgacgc cttacgccat ctctcaaccg
    21421 aaattattca ggcgcgtttt ctcgcctctg gcctgaaatg ccgttattac aatccggcaa
    21481 tccatacggc agcttttgcc ttacctcagt atctgcaaga cgcactggct tcacagccgt
    21541 cctaaggaga agataagaaa ttgaaaaaac tgaaactgca tggctttaat aatctgacca
    21601 aaagtctgag tttttgtatt tacgatatct gctacgccaa aactgccgaa gagcgcgacg
    21661 gttatattgc ttatatcgat gaactctata atgccaaccg tctgaccgaa atcctgtcag
    21721 aaacctgttc cattatcggg gctaatattc ttaacatcgc ccgccaggat tacgaaccac
    21781 agggtgccag cgtcactatt ctggtgagtg aagaaccggt tgacccgaaa ctcatcgaca
    21841 aaacagaaca ccccggccca ctgccagaaa cggtcgttgc ccatcttgat aaaagtcata
    21901 tttgcgtaca tacctacccg gaaagtcatc ctgaaggcgg tttatgtacc ttccgcgccg
    21961 atattgaagt ctctacctgc ggcgtgattt ctccgctgaa ggcgctgaat tacctgatcc
    22021 accagcttga gtccgatatc gtaaccattg attatcgcgt gcgcggtttt acccgcgaca
    22081 ttaacggtat gaagcacttt atcgaccatg agattaattc gattcagaac tttatgtctg
    22141 acgatatgaa ggcgctgtat gacatggtgg atgtgaacgt ctatcaggaa aatatcttcc
    22201 ataccaagat gttgcttaaa gagttcgacc ttaagcacta catgttccac accaaaccgg
    22261 aagacttaac cgacagcgag cgccaggaaa ttaccgctgc gctgtggaaa gaaatgcgcg
    22321 agatttatta cgggcgcaat atgccagctg tttaacggct ctggcggagc tcccaggctc
    22381 cgccagattt atttacttct gctgcacgaa attacggtaa gccgccacga cctgcagaaa
    22441 atcctcaacg ccgcatagcg acaggctttc ttcgtcgtag tagttcatcc cctcttccat
    22501 ttcatcgcca gcgaattcca gttgattggc gcgaaccatc acctcttcac cgtccatcca
    22561 cagggtgtat tcatgccctg cccgttgcca ggaccgttcg ctacctttca gtgcgtgcgc
    22621 tgcttgttcc acttcatcaa gcaaggccag gttttctttc acctcttcat taaaccagtg
    22681 cccgaccact tcatgcccca tggacatacg cacctttacc actccggtgg tatcgcgcag
    22741 aaattcgtaa tccatagtgt gttcctctgc tcccggcaat gcgccgaaat cgcattgcgc
    22801 cattgatgta attatcgcag ccttaccgga gaaaaacagc ggaacggcga tggtttagaa
    22861 ataaaaaatg cccgggaatc tcattcaccg ggcattgtgt cgtttatgcg cagcgcgtgc
    22921 gctgactttt taaactgcag tctggaaaat cacaccatcg gctttttcag tgtactgaga
    22981 aagctggttg aagttcaggt aacggtaagt atcaacggca gttttatcca cctgcgccac
    23041 gtaggtctgg tactcttccg gcgtcggtag tttgccaatc agcgccgcaa cagcagccag
    23101 ttccgcagaa gccaggaaga cattcgcgcc agtccccaga cggttcggga agttacgggt
    23161 agaggtggaa accaccgttg caccgtccgc cacacgcgcc tggttaccca tacacaggga
    23221 acagccaggg atctcgatac gcgcaccact cttaccgaag acgctgtagt agccttcttc
    23281 ggtcaactgt gcggcgtcca tacgggttgg cggtgccacc cacaggcggg tcggcaactg
    23341 gcctttatgc gcatccagca gtttacccgc agcacggaag tgaccgatgt tggtcatgca
    23401 ggaaccgata aacacttcgt cgatcttctc accctgtacc gcagacagcg gacgcgcgtc
    23461 atccgggtcg ttcggagcac acaggattgg ctctttaata tccgccagat cgatgtcgat
    23521 cactgccgcg tattccgcat ctgcatcggc ttccagcagc tcaggattcg ccagccattt
    23581 ttccatgccc tgaatacgac gttccagggt acgacgatcg ccgtaacctt ccgcgatcat
    23641 ccacttcagc aggacgatat tagagttcag gtattcgatg atcggttctt tgttcagctt
    23701 gatggtacaa ccggcagcag aacgctcggc ggacgcatcg gtcagttcaa atgcctgctc
    23761 aactttcaga tccggcagac cttcaatttc cagaatgcga cctgagaaga tgtttttctt
    23821 gcctttcttc tcaacggtca gcagaccttg tttgatcgca tacagcggaa tagcgtgtac
    23881 cagatcgcgc agggtgatgc ccggctgcat tttgcctttg aagcgcacca gaacggattc
    23941 cggcatatcc agcggcatta cgcctgttgc ggctgcaaat gccaccagac cagaacccgc
    24001 cggaaaagag ataccgatcg ggaaacgggt atgggagtca ccaccggtac cgacggtatc
    24061 cggcagcagc atacggttca gccaggagtg gattacgccg tcacccggac gcagcgacac
    24121 accgccacgg ttcataatga agtccggcag cgtgtggtgc gtgttcacgt caaccggctt
    24181 cggatacgcc gctgtgtggc agaaagactg catcaccagg tcagccgaga agcccaggca
    24241 cgccaggtct ttcagttcat cacgggtcat cgggccggtg gtgtcctggg aacctacaga
    24301 agtcattttc ggttcacagt acgcgcccgg acgaatgcct ttcacgccac aggcacggcc
    24361 taccattttc tgcgccagcg agaagccgcg atcgctctca gcgacatctt tcgcctgacg
    24421 gaacacatca ctgtgcggca gaccaagtgc ttcacgcgct ttggtggtca ggccacgccc
    24481 gataatcagc ggaatacggc cgccagcacg cacttcatca atcagcacgt cggttttcag
    24541 ttcgaaggtc gccagcagtt cgcctgtttc gtggttacgc acttcacctt tgtacgggta
    24601 aacgtcaatc acgtcgccca tgttcaggtt agagacgtcg acttcgattg gcagtgcacc
    24661 cgcgtcttcc atcgtgttaa agaagattgg tgcaatttta ccgccgaggc acaaaccacc
    24721 gccgcgtttg ttcggcacat gtggaatatc atcgcccata aaccacagaa cggagttggt
    24781 ggcggattta cgcgatgaac ccgtacccac aacgtcaccg acgtacgcca gcgggaaacc
    24841 tttctgttgc agagcttcaa tctgtttgat cgggccaaca acacctggct ggtctggctc
    24901 aataccttca cgggcgtttt tcagcatcgc cagcgcgtgc agtgggatat ccgggcgcga
    24961 ccacgcatcc ggtgccggag aaaggtcatc ggtgttggtt tcgccagtga ctttgaagac
    25021 ggtaacggtc agtttttcag ccagcgccgg gcgattcagg aaccattcgg catccgccca
    25081 ggactgcatt acctgcttcg catattcgtt gcctgctttg gctttctctt ctacgtcata
    25141 gaagttatcg aacatcagca gcgtgtgaga aagtgctttg gcagcgatcg gtgccagttt
    25201 ggcatcatcc agcgcgtcga tcagcggatg aatgttgtaa ccaccctgca tggtgcccag
    25261 cagttcgatg gctttttccg gagtcagcag tggggatttg gcttcacctt tcgcgacagc
    25321 agccaggaag cctgctttga cataggcggc ttcatcgacg ccagggggaa cacggttggt
    25381 taatagatct aacaggaatt ctttttcgcc cgcgggcggg tttttcagca gctctacaag
    25441 tgcggccatt tggtttgcat ccaggggttt gggcgcaatc ccctcagcgg cacgctcagc
    25501 tacgtgctta cggtattctt ctagcacgac ggttctcctc gctctcattg tcatagtgcg
    25561 gcaggcgatt ctcttcacgc tcctgtgaga cagcagtttg tagggtaaat gcccagtacc
    25621 gcaacgggca gaatagcagg attttgacga tgtgttaatc tgtttacaaa aaagcaacat
    25681 aaaaaagtgg ctgaatcgtt aaggatggtc tagagattgt ttccctccgt aaaaacttcg
    25741 ttaattcgca tagtgataat cacctttctc aacgaatccc aagattagtc aaaatttaaa
    25801 ctaccgcctc tttatactca gatttacagc acctgcgggt ggcagttcgc cgaccattgc
    25861 gatttccttg agatccgaat tatgaaaatg actttgccgt ttaaacccca tgtgctggcg
    25921 ctaatttgca gtgccgggct ttgtgccgcc tctgccgggc tatatataaa aagccgcaca
    25981 gtggaagcgc ctgtggaacc gcaatcgaca caacagactg cgcctgacat caccgcagtt
    26041 acgcttcctg caacggtttc cgcaccgccc gtaacgcccg ccgtcgttaa atccgcattc
    26101 agcactgcac aaatcgatca atgggttgca cctgtcgcgc tataccccga ctctctgctt
    26161 tcacaagtgt taatggcatc aacctatccg gcaaacgttg ctcaagcagt gcaatggtcg
    26221 cacgataatc cacttaaaca aggcgatgct gctattctgg cggtatctga ccagccgtgg
    26281 gacgctagcg tcaaatcact ggtggccttt ccacaattga tggcgttgat gggcgaaaac
    26341 ccgcaatggg tgcaaaacct gggcgatgct tttctggcgc agccgcagga cgtgatggac
    26401 tcggtacagc gattgcggca actggcgcaa caaaccggct cgctgaagtc atcaaccgaa
    26461 cagaaagtta ttaccacaac gaagaaaacc gtaccggtaa cacagacagt cacggctccc
    26521 gtcataccat ccaataccgt ttcaactgcc aaccccgtca ttacagagcc tgcaacaacc
    26581 gtcatttcca ttgagcccgc caatcctgat gtggtctata ttcccaacta caacccaacc
    26641 gtggtttacg ggaactgggc caatactgcg tatccgccgg tttatctgcc accaccagcc
    26701 ggagaaccgt ttgttgacag ctttgtgcgc ggattcggct atagcatggg cgttgctacc
    26761 acgtacgcac tattcagcag catcgactgg gatgacgacg atcatgacca tcatcatgac
    26821 gatgattatc atcaccacga tggcggtcat cgtgacggta atggctggca acacaacggc
    26881 gacaacatca atatcgacgt caacaatttc aaccgtatca ccggtgagca tcttactgat
    26941 aagaatatgg catggcggca caatccaaac taccgtaatg gtgtgcccta tcatgatcag
    27001 gatatggcaa agcggtttca tcaaaccgat gtcaacggcg gaatgagtgc cacgcagcta
    27061 cctgctccaa cacgcgatag ccagcgtcag gcggcagcaa gtcagtttca gcaacgaaca
    27121 cacgccgccc ccgtcattac acgagatacc caacgtcagg cagcggcaca gcggtttaat
    27181 gaagctgaac actatgggag ctatgacgac ttccgcgact tcagccgtcg ccaaccactg
    27241 acccagcaac aaaaggacgc cgctcgtcag cgttatcagt cagcttctcc tgagccgcgc
    27301 caggcagttc gcgagagaat gcagactaac ccgcagaacc agcagcgaag agaggcagcg
    27361 cgtgagcgca ttcagcccgc ctcgcctgag cagcgccagg cagtccgcga gaaaatgcag
    27421 actaacccac agatccagca gcgaagagag gcagcgcgtg agcgtattca gtcagcctcg
    27481 cctgagcagc gccaggtgtt taaggaaaaa gtacagcaga gcccactgaa ccaacagcaa
    27541 cgtgataacg cccgccagcg tgttcaatcg gcatcacctg aacaacgtca ggtttttcgg
    27601 gagaaagtta aggagagcca cccacaacgt ctaaacgaca gtaaccatac tgccaggcta
    27661 aataacgagc aacggtcagc agtacgcgaa cgtctctctg agcgcggagc aaggcgactg
    27721 gaaaggtaaa ttacagacgt aaaaaaagcg gcgtggttag ccgctttttt aattgccgga
    27781 tgttccggca aacgaaaaat tacttcttct tcgctttcgg gttcggcagg tcggtaatgc
    27841 taccttcgaa cacttctgcc gccaggccca cagactcgtg cagagtcggg tgcgcatgga
    27901 tggtcagcgc gatgtcttca gcgtcacaac ccatttcgat tgccaggccg atttcaccca
    27961 gcagctcgcc gccgttggta ccgacaatcg caccaccgat cacacggtga gattctttgt
    28021 cgaaaatcag cttggtcata ccgtctgcgc agtcggaagc gatagcacga ccagaagcag
    28081 cccacgggaa ggtggcggtt tcatagctga tgcctttctc tttcgcttct ttctcagtca
    28141 gacctaccca tgcaacttct ggttcggtat aggcgatgga cgggataact ttcggatcga
    28201 agtagtgttt cttaccggcg ataacttcag cggcaacgtg accttcgtga acacctttgt
    28261 gtgccagcat cggctgaccg acgatatcgc cgatagcaaa gatgtgcggt acgttggtac
    28321 gcagctgttt gtcaacgcgg atgaaaccac gatcgtcaac ttccacgcca gctttgcctg
    28381 cgtcgaggtt tttaccgttc ggcacacgac caatcgctac cagcactgcg tcgtaacgct
    28441 gcggttcagc tggtgctttt ttgccttcca tcgtcacata aataccgtct tctttcgctt
    28501 caacggcggt aactttggtt tccagcatca ggttgaattt cttgctgata cgcttggtga
    28561 agactttaac gatgtctttg tcagctgccg ggataacctg gtcgaacatt tcaaccacgt
    28621 caatctgtga acccagcgcg tggtaaacgg tgcccatttc cagaccgatg ataccgccac
    28681 ccattaccag caggcgttct ggtacttctt tcagttccag cgcgtcagtg gagtcccaga
    28741 tacgcggatc ttcatgcgga ataaacggca gttggatcgg gcgagaaccc gctgcaatga
    28801 tcgcgttgtc gaagttgatc acggttttac cgttctcacc ttcaacttcc agggtgttag
    28861 ccccggtaaa tttacccaga ccgttgacca ctttgacttt gcggcctttc gccataccag
    28921 ccagaccacc ggtcagctga ttgatcactt tctctttcca ggtacgaatc ttgtcgatat
    28981 cggttttcgg ttcgccgaag acgataccgt gttcagccag cgctttggct tcttcgataa
    29041 cttttgctac gtgcagcagt gctttagaag ggatacagcc gacgttcagg caaacaccgc
    29101 caagggtgtt gtaacgttct acgattacgg tttccagacc taaatcagcg caacggaagg
    29161 cagcggagta acctgcgggg cctgccccaa gtaccacgac ctgagttttg atttcagtac
    29221 tcatcatgac ctctatatat ttatctccgg cgggtctgac gtttttttta tgacgcccat
    29281 tatccaccgg gtcgttctat ccgtcggtat tttacaaaat tgttaacaat ttttaaacaa
    29341 caaacggcaa ccgatttgtc tattcgctaa taacctcaat acattcatga gattaccaga
    29401 aaaaagccgg ccgttgggcc ggctctttta cttacatcac cagacggcga atgtcagaca
    29461 gcgtgttgtt aatgatggta atgaaacggg caccatcagc accgtcgatc acgcggtggt
    29521 cgaaggagag agaaatcggc agcatcagac gcggcacgaa ctctttacca ttccacaccg
    29581 gctccatcgc ggacttggaa acgccgagga tagccacttc cggcgcgttc acaatcggcg
    29641 cgaagtgggt agtacccagg ccgccgatgc tggagatggt gaagcaaccg ccctgcattt
    29701 cgcccgcagt cagcttaccg tcacgcgctt tcttagaaat agtcatcagc tcgcgagaca
    29761 gctcgatgat gcctttcttg ttgacgtctt tgaataccgg aacaaccaaa ccgttcgggg
    29821 tatccaccgc cacaccgatg ttgatgtatt tcttcagggt cagacgctga ccgtcttccg
    29881 acagcgaact gttgaagcga ggcatctgct caagagctgc agcaacggct ttcatgatga
    29941 agacaaccgg ggtgatcttc acatccagct tacgtttcgc cgcttcttcg ttctgctgtt
    30001 tacggaacgc ttccagctcg gtgatatcgg ttttgtcgaa gtgagtaaca tgcgggatca
    30061 tcacccagtt acggctcagg ttcgcaccag aaattttctg gatacggccc agttccactt
    30121 cttcgatttc accaaacttg ctgaagtcca ccttcggcca cggcagcatg cctgggatac
    30181 cgccgccagt cgccgccgga gctgcttctg cacgtttgat agcttctttc acgtaagcct
    30241 gaacgtcttc gcgcaggata cgacctttac ggccagtgcc cttcactttc gccaggttaa
    30301 cgccaaactc gcgtgccaga cggcggatca gcggagtcgc gtgaacataa gcgtcgtttt
    30361 cagcaaattc agatttgcct tccgctttcg cagctggtgc tgctgccggg gcttcagctt
    30421 ttgctgccgg agccggcgct gccgcttcct gtttcgcagg agctgccgca ggcgctgcgc
    30481 cttcaacttc gaagatcata atcagcgagc cagttttcac tttatcgcca acgttgactt
    30541 tcagttcctt cacgacgcct gcaaacggag ccggaacttc catagaagct ttgtcgcctt
    30601 ctacggtaat cagtgactgt tcagcggcaa ctttgtcgcc cactttcacc atcacttcgg
    30661 tcacttcaac ttcgtcaccg ccgatatccg gaacgttaac ttctttcacg ccagctgctg
    30721 gtgcaggggc cgctgccgga gccgcttcct gtttagccgc cggagctgcc gcgcctgctt
    30781 cacccgcgac ttcgaagacc ataatcagcg agccggtaga cactttgtcg ccggtgttca
    30841 ctttgatttc tttcacggtg cctgcaaacg gagccggaac ttccatagaa gctttatcgc
    30901 cttctacggt gatcagcgac tgctcagctt caactttgtc gccaactttc accaggattt
    30961 cggtcacttc aacttcgtcg ctgccgatat ccggaacgtt aacgtctttt gccgcggcag
    31021 ccgcgggtgc tgctgccgga gctgcttctt tcttctcttc tgcctgagca ggtgcagcgt
    31081 ctgctgcacc gtcggcggaa tcgaaaatca taatcagtgc gccggtctgg gttttatcgc
    31141 caacagagac tttgatctct ttaacgatac ccgcctgcgg agacggaact tccatagagg
    31201 ctttgtcgcc ttctacggtg atcagcgact gttcggcttc aactttgtcg cccactttga
    31261 ccaggatctc ggtgatttca acttcatcag ccccgatgtc cggtactttg atttcgatag
    31321 ccattattct tttacctctt acgccagacg cgggttaact ttatctgcat cgatgttgaa
    31381 tttggcgatt gcgtcagcaa ccactttctt atcgatttcg ccacgtttag ccagttcgcc
    31441 cagcgccgca accacgacat aagaagcatc aacttcgaag tggtgacgca ggttctcacg
    31501 gctgtcggaa cgaccgaagc catcagtacc cagtacgcgg tagtcgtcag ccggtacgta
    31561 agtacggacc tgctcagcga acagtttcat atagtcggta gatgccactg ccggagcgtc
    31621 gttcatcacc tgcgcgatat acggaacgcg cggagtttcc agtgggtgca gcatgttcca
    31681 gcgttcacaa tcctgaccat cacgcgccag ctcggtgaag gaggtcacgc tataaacgtc
    31741 agaacctacg ccgtaatctt tcgccaggat ctctgccgct tcacggacgt gacgcaggat
    31801 agaaccggag cccagcagct gaactttacc tttgctacct tcaatggttt cgagtttgta
    31861 gataccttta cggatacctt cctcagcacc ttccggcatt gccggcatgt ggtagttttc
    31921 gttcagcgtg gtgatgtagt agtaaacgtt ctcttgtttt tcaccgtaca tacgctccag
    31981 accgtcatgc ataatgacag caacttcgta agcgtaagcc gggtcgtaag agatacagtt
    32041 cggaatagtc agcgactgaa tatggctgtg accatcttcg tgctgcagac cttcaccgtt
    32101 cagggtggta cgaccggaag taccgccgat caggaagcca cgcgcttgct ggtcgccagc
    32161 cgcccagcac agatcgccga tacgctggaa gccgaacatc gagtaataga tgtagaacgg
    32221 aatcatcggc agattgttgg tgctgtaaga ggtcgccgct gccagccagg aacaacctgc
    32281 gcccagctcg ttgatccctt cctgcagaat ctgacctttc tcgtcttctt tatagtaagc
    32341 aacctgctcg cggtcctgcg gggtgtactg ctgaccgttc gggctgtaaa taccaatctg
    32401 acggaacaga ccttccatac cgaaagtacg cgcttcgtcg gcgatgatcg gtaccagacg
    32461 atctttgatc gacttgttct tcagcatcac gttcagggca cgaacgaaag cgatagtggt
    32521 agagatctct ttgctttgct cttccagcag cgcgccgaag tcttgcaggc tcggcagctc
    32581 aagcttctcg gtgaagttcg gctgacggct tggcagataa ccgtgcagtt tctgacgctg
    32641 agcgtgcaga taggtatgct cttcagaacc ttccgggaag gtgatgtacg gcagtttttc
    32701 gatatctgca tcagacaccg gcacattgaa acggtcgcgg atatgacgca caccgtccat
    32761 gttcattttc ttaacctggt gcgcgatgtt tttaccttca gccgcgtcgc ccatgccgta
    32821 acctttaatg gtatgagcaa ggattactgt cgctttgcct ttggtttcct gcgctttctt
    32881 gaatgcagcg tagattttct tcggatcgtg accaccacgg ttcagtgccc agatctgctc
    32941 gtcagtccag tctgcaacca gtgctgcggt ttcaggatat ttaccgaaga agtgttcacg
    33001 aacgtacgca ccatctttcg atttgaaggt ctggtagtcg ccgtcaacgg tttcgttcat
    33061 cagctggatc agtttaccgc tggtatcttt acgcagcagt tcatcccaac ggctacccca
    33121 catcactttg atcacgttcc agccagcacc ttcgaagatg ccttccagtt cgttgatgat
    33181 cttgccgtta ccggtgaccg ggccgtcaag acgctgcagg ttacagttga taacgaagac
    33241 caggttatcc agtttttcac gggtagcgat ggtgatcgca cctttggatt ccggttcgtc
    33301 catttcaccg tcgccgagga acgcgtaaac ggtctgttta gaggtatctt tcaggccacg
    33361 gtgttccaga tatttcagga atttagcctg gtaaatagca ccaatcggac ccagacccat
    33421 agatacggtc gggaactgcc agaattccgg catcagtttc gggtgcggat aggaagagag
    33481 gccattgccg tgaacttcct gacggaagtt atccagctgc tcctgagtca gacgaccttc
    33541 caggaaagca cgagcgtaaa cgcccgggga gatgtggccc tggaagtaaa ccaagtcgcc
    33601 gccatcctgc tcgttgcgtg cacggaagaa gtggttaaag cacacatcat aaatggttgc
    33661 ggaagactgg aaggacgcca tgtggccgcc cagttcgagg tcttttttcg acgcacgcag
    33721 aaccgtcatg atggcgttcc agcggatagc tgaacgaata cggcgttcca gttccagatt
    33781 acccggatac tccggttgtt cttcaacggg gatggtgttg atgtagttgc tgataccggt
    33841 gcctgcggct acgttgacac cgcctttgcg ggcttcagca agcagttggt cgatcagata
    33901 ctgagcacgc tcaacacctt cttcacggat gaccgattcg atcgcctgga gccagtcgcg
    33961 agtttcgatc ggatccacgt catttgggaa acgttctgac atgggttatt ccttatctat
    34021 ctaataacgt tgagttttct ggaacctgtc ccattgaact ctcgccggaa agctcaataa
    34081 gacaggttct acgtttagtt gccgcgcttt tatatgcgct tgatttacaa catcttctgg
    34141 ataattttta ccagaaaaat cactaattct ttcgttgctc cagacgacgc agagaacgct
    34201 cacggcggct ctcttcacgg cttctgtcga gcaaaatttc ttcgataaag gccagatggc
    34261 gatgcgatgc ttcgcgcgct tcttccggct taccggccat aattgcttcg aatatacggg
    34321 tgcggtgact actcaccagc ggcagcatct cgcgacgcgc atagagcaat tcgaagttct
    34381 gacggacatt ctgggccaac atcggctcca tacaccttag cagatgaagc aaaaccacat
    34441 tgtgggctgc ttcggtgacg gcaatctgat actggagtac ggcgtttgat tccgcgtcca
    34501 gatcgccaga ctgctgcgcc agttctatgg cgtggtggag ttcacggatg cgttccttgt
    34561 cttcatcggt actacgcagc gcggcgtaat aagcggcgat accttccagg gcgtgtcgtg
    34621 tttcgagcaa gtcatactgt gattcaggat ggtcggagag cagctctacc agcggatcgc
    34681 tgaagctttg ccataggctg ctctggacaa aagtgccgcc accctggcga cgaagcaaca
    34741 agcccttcgc ttcgagacgt tgaatcgcct cacgcaagga gggacgggag acgtcaaact
    34801 gttttgccag ttcgcgttcc ggtgggagtt tttcgcccgg gcggagagtg ccttcgagga
    34861 tcaaaaactc cagttgctgc tcaatcacat cggagagttt tggttggcgg attttgctgt
    34921 aggccatgag ttcctgtctt aagccacttg ccgaagtcaa ttggtcttac caatttcatg
    34981 tctgtgacgc taaagtaaca aagtattcac cttatgtcca tacaggtttt gattgaaatc
    35041 atgaaactgt gcacatttta acaacttgac atatataacg tttcaaagtt gtaactatgc
    35101 acaaatgtag actttacgta ggaaagggat tttttaacga ttattaaact ataaaaatcc
    35161 gaattgaacc gattcactta ccaattttgt gatttttaat tcagttaaaa cgaatttaaa
    35221 ttcattctgc atattgagag gggttgaggc tgagatttac aaacggtttc tttttaagca
    35281 actcatcttc aaccatgcat aaagcgggtg cattcgctgc cgcataccat tattcttgat
    35341 ctgacggaag tctttttgta acaattcaaa cttctttgat gtaaacaaat taatacaaca
    35401 aacggaattg caaacttaca cacgcatcac tgcgtagatc aaaaaaacaa tcaccgcacg
    35461 aggtttcatg atggaaggtc aacagcacgg cgagcagcta aagcgcggcc ttaaaaaccg
    35521 ccatattcag cttatcgcgc tgggtggcgc gatagggacc gggttattcc tgggtagcgc
    35581 ctccgtaata cagtccgcag ggccagggat tatcctgggt tacgccattg ctggttttat
    35641 cgcctttctg atcatgcgtc agctgggtga aatggtggtc gaagaacctg tcgcaggctc
    35701 ctttagccac tttgcttata aatactgggg cagctttgct ggcttcgctt ctggctggaa
    35761 ctactgggta ctgtacgttt tagttgccat ggcagagctg actgctgtgg gtaaatacat
    35821 tcagttctgg tatccggaaa tcccaacctg ggtttctgcc gccgtgttct ttgtggtgat
    35881 taacgccatc aacctgacca acgtaaaagt gtttggtgag atggagttct ggtttgccat
    35941 tatcaaagtt attgcggtag tagcgatgat catcttcggc ggctggctgc tgttcagtgg
    36001 taacggcggt ccgcaggcaa gcgttagcaa cctgtgggat cagggtggtt tcctgccgca
    36061 cggcttcacc gggctggtga tgatgatggc gattatcatg ttctcgttcg gtggtctgga
    36121 actggtgggg atcaccgcag cagaagctga taacccggag caaagtatcc cgaaagcgac
    36181 taaccaggtt atctaccgca tcctgatttt ctatattggt tcgttagccg ttctgctctc
    36241 actgatgccg tggacccgcg ttaccgccga taccagtccg tttgtgctga tcttccacga
    36301 gttaggcgat acctttgtgg cgaatgcgct gaacatcgtg gtgctgactg cggcgctctc
    36361 cgtgtacaac agctgcgtat attgcaacag ccgtatgctg tttggtctgg cacaacaggg
    36421 taatgcgcca aaagcgctgg cgtctgtcga taaacgcggc gtaccggtta acaccattct
    36481 ggtgtctgca ctggtaacgg cattgtgcgt actgattaac tatcttgctc cggaatccgc
    36541 atttggcctg ttaatggcgc tggtggtatc tgcactggtg atcaactggg cgatgattag
    36601 cctggcgcat atgaaattcc gtcgcgccaa gcaggaacaa ggcgtggtaa ctcgcttccc
    36661 tgctctgctt tatccgctgg gtaactggat ctgcctgctg tttatggcgg cggtactggt
    36721 gattatgctg atgaccccag gaatggcgat ttcggtatac ctgatcccgg tatggctgat
    36781 cgtgttaggt atcggctatc tgtttaaaga gaaaaccgcc aaagccgtaa aagcgcatta
    36841 atctctctac gccctcacct gtacacggtg agggcaataa tctttacacc aacgccccgt
    36901 aaatcgtcag tagcgcaatc accaccacga ccacgaacga ggttttcttc gccattgaaa
    36961 ccgctgcctt cggcgtctcc accttatcga catgcggttc acgcgccaga gagaactgcg
    37021 ccagacgcgt taacacctga tactgcgaag tatggaaatc acccagcgaa gcaaaccagg
    37081 ccggtaacgc tttctcacca tgaccgatca aggcatatac cacacccgca agacgaactg
    37141 gcacccaatc cagtacatga agcacggcat caatgccgga ctgtaaacga tgatgcggcg
    37201 tctggtatcg cgccagccag tattgccatg cacgcaagaa cgcatacccc atcagcgtaa
    37261 cgggtcccca ggttcccccc acaatcagcc agaacagcgg tgcaagataa aaacgaaagt
    37321 taatccacag caatgcattt tgcagctcac gcaaatactc acgttcgtcg cagcctgccg
    37381 ggacgccgtg aatcatggtg agttcgccag ccatcgtggc acgggcatgg ctatcattac
    37441 gtgaagcaac tgtcagataa gcatgataat gaagacgaac tttacctgcg ccaatacaca
    37501 gcaaaccaat cagcagccac accagcagcg tgggaacgtt gaacaatacg ccctgcaatg
    37561 cgcgtaacag taaaaaagtc acgcccatcg caataatggt catgcctaac gtgcgcccga
    37621 gagaaaaatg tttcacccgc cgaaagaacg cttcaagacg atgatcaagc tgccagtgct
    37681 cgcccaactt aaacaggcgc tcgaaaatta acaccagtaa ggttgtaaat agcgtcatgt
    37741 tgtctccttg ctgaccagcg cacgaaaccg tgcccaatca aatgcaggac cgggatcggt
    37801 tttccgatcc ggcgcaatat cacaatggcc cgtcatgtta ttagagatat ccggatagca
    37861 atcaatcagt gcccgcgtaa ccgccgcaag ctgttgatac tgcgcatcgg tatacgccag
    37921 cgtatcggtg ccttcaagct caatcccaat agaaaaatca ttgcagcgtt cgcgcccctg
    37981 atactgagag actcccgcat gccatgcacg tttatcgaaa ggaacatact ggactatttc
    38041 accatcacgg cgaatcaaac agtgagcgga gacgcgcaaa tgggcgatct cagcaaagaa
    38101 aggatgtgcc tgcggatcaa tagttccagt gaataatgcg tcgatccacg gaccgccaaa
    38161 ctcgcctggc ggcaggctaa tattgtgcac caccagcagg gtgggtgttt cgtcatccgg
    38221 gcggcaatcg taatgtggtg agggaacgcg gcgcgcgcca accagccacc cctgttctaa
    38281 caacatgcag gatctcctta tatgtggtgc taatacccgg ttcagagtag catgtttcta
    38341 ccttatgatt cgttagctat ctggagtttt aacatgccgc ctcgccgcta taaccctgac
    38401 acccgacgtg acgagctgct ggaacgcatt aatctcgata tccccggcgc ggtggcccag
    38461 gcgctgcggg aagatttagg cggaacagtc gatgccaaca atgacattac ggcaaaactt
    38521 ttaccggaaa actctcgctc tcatgccacg gtgatcaccc gcgagaatgg cgtattttgc
    38581 ggcaaacgct gggttgaaga ggtgtttatt caactggcag gcgacgatgt caccataatc
    38641 tggcatgtgg atgacggcga tgtcatcaac gccaatcaac ccttgttcga acttgaaggc
    38701 ccatcccgcg tgctgttaac gggcgaacgc actgcgctta attttgtgca aaccctttca
    38761 ggagttgcca gtaaggtacg ccactatgtc gaattactgg aaggcaccaa cacgcagttg
    38821 ttggatacgc gcaaaacctt acccggcctg cgttcagcgc tgaaatacgc ggtactttgc
    38881 ggcggcggag cgaatcaccg tctggggctt tctgatgcct tcctgatcaa agaaaaccat
    38941 attattgcct ccggctcagt gcgccaggcg gtcgaaaaag cgtcctggct gcacccggat
    39001 gcgccagtag aagtcgaagt agagaatctg gaagaacttg atgaagccct gaaagcagga
    39061 gccgatatca tcatgctgga taacttcgaa acagaacaga tgcgcgaagc cgtcaaacgc
    39121 accaacggca aggcgctact ggaagtgtct ggcaacgtca ctgacaaaac actgcgcgaa
    39181 tttgccgaaa cgggcgtgga ctttatctcc gtcggtgcgc taactaagca cgtacaggca
    39241 ctcgaccttt caatgcgttt tcgctagttg ctgacagatt tatccgctcc atagtgggcg
    39301 gataatcacc ccttccccaa ctttcttcgt aacgcctcgc aaatttcttg tctttcgctg
    39361 caacggaata aaaaacctcg gaagctgctt tcccgtttct ttttttgccc ctcgcttgtg
    39421 ctgatggcgt ttgccaaagt agcgccaacc caatcaagga gcgaaacaga tggacaagca
    39481 acgcggtttt acacttatcg aactgatggt ggttattggc atcattgcca ttttaagcgc
    39541 cattggtatt cccgcttatc aaaactacct gcgcaaagcc gcactcaccg acatgctaca
    39601 aacctttgtg ccttaccgta ccgccgtaga gttgtgcgcg ctggaacatg gtggattaga
    39661 tacctgcgac ggtggcagca atggcattcc ctcgcctacc accatccgct atgtttcagc
    39721 catgagtgtg gcaaagggcg tggtgtcgct gaccgggcaa gaaagtctca atgggctaag
    39781 cgtcgtcatg acgccaggtt gggataacgc aaacggcgtc accggctggg cgcgcaactg
    39841 caatattcaa agtgacagcg cattgcagca agcctgcgaa gatgtcttcc gctttgatga
    39901 cgccaactaa ggagcggcaa tgaatattcc acagctcacg gccctgtgtc tgcgttatca
    39961 gggagtcttg ctggatgcca gcgaagaagt ggttcatgtt gcggtggtcg atgccccctc
    40021 acatgagttg ctggacgcat tgcatttcgc taccaccaaa cgtattgaga tcacctgctg
    40081 gacgcgccaa caaatggaag gtcacgccag tcgcacacaa cagacattgc ccgtagctgt
    40141 tcaggagaag catcagccca aagcagagtt gctgactcga acgttacaat ctgcgctgga
    40201 acaacgcgcg tctgatattc atatcgaacc agcggacaat gcctaccgca tccgcttgcg
    40261 tatcgacggc gtattgcatc ctttaccgga tgtttcaccg gatgccggag tcgcattaac
    40321 cgccagatta aaagtgctgg gaaacctgga tattgcggaa catcgcctgc cgcaggacgg
    40381 gcaattcact gtcgaactgg caggaaacgc cgtctcattt cgtattgcga ccttaccatg
    40441 tcggggtggt gaaaaggtgg tattaaggtt gttacagcag gtgagccagg cactggatgt
    40501 caacacgctg ggaatgcagc cgttacaact ggcggacttt gctcatgcct tgcaacaacc
    40561 acagggactg gtgctggtaa ctggccctac cggcagcggc aaaacggtca cgctttatag
    40621 tgccctgcaa acgctgaata ccgctgacat taatatttgt agcgtcgaag atccggttga
    40681 gatccccata gccggactaa accagacgca aatccatccg cgtgccggac tcacctttca
    40741 gggcgttttg cgtgcgttat tgcgccagga tcctgacgtc atcatgatcg gagagatccg
    40801 cgatggcgaa acagcagaga tcgctattaa agccgcgcaa accggtcacc tggtgttgtc
    40861 taccctacac accaattcca cctgcgaaac gctggtacgt ttacagcaaa tgggagtcgc
    40921 ccgctggatg ctctcatcag cgcttacgct ggtaatagcc cagcgtctgg tacgtaaact
    40981 ttgcccacat tgtcgccagc agcaagggga gcccatccat attccagaca atgtatggcc
    41041 gtcgccgctg ccccactggc aggcacctgg ttgtgtacat tgctaccacg gtttttatgg
    41101 tcgcacggcc ttatttgaag ttctgcccat aacaccggtc atacgtcagc ttatttccgc
    41161 taataccgac gttgaatcgc tggaaactca cgcccgacag gcgggtatgc gtacgctttt
    41221 tgaaaacggc tgcctcgccg tggagcaagg cttaactacc tttgaagagt taatccgcgt
    41281 actggggatg ccgcatggcg agtaagcaac tctggcgctg gcatggcata accggcgacg
    41341 gcaatgcgca agatgggatg ctatgggcag agagccgtac tttactgctt atggcactac
    41401 agcaacagat ggttacccca ctaagcctga agcgaatcgc catcaattct gcgcagtggc
    41461 ggggagataa aagcgcggaa gtcattcatc aactggcgac gctactcaaa gcagggttaa
    41521 cgctttctga agggctggct ctgctggcgg aacagcatcc cagtaagcaa tggcaagcgt
    41581 tgctgcaatc gctggcgcac gatctcgaac agggcattgc tttttccaat gccttattac
    41641 cctggtcaga ggtatttccg ccgctctatc aggcgatgat ccgcacgggt gaactgaccg
    41701 gtaagctgga tgaatgctgc tttgaactgg cgcgtcagca aaaagcccag cgtcagttga
    41761 ccgacaaagt gaaatcagcg ttgcgttatc ccatcatcat tttagcgatg gcaatcatgg
    41821 tggttgtggc aatgctgcat tttgttctgc cggagtttgc cgctatatat aagaccttca
    41881 acaccccact accggcacta acgcagggga tcatgacgct ggcagacttt agtggcgaat
    41941 ggagctggct gctggtgttg ttcggcatta tgctgacgat agccaataag ttgctgatgc
    42001 gccgaccgac ctggcttatc gcgcggcaga aattgctgtt acgcttcccg attatgggtt
    42061 cactgatgcg gggacaaaaa ctcacgcaga tctttacgat tctggcgctg actcaaagtg
    42121 caggcattac ttttttacag ggcgtagaga gcgtcagaga aacaatgcgc tgcccgtact
    42181 gggtgcaact tctgacacaa atccagcacg atatcagtaa cggtcatccc atctggctgg
    42241 cgctaaaaaa tgccggtgag tttagcccgc tctgtttgca attagtgaga acgggagagg
    42301 cctccggctc gctggatctc atgttagaca acctcgccca tcatcatcgg gataacacaa
    42361 tggcgctggc ggataaccta gcagccttac tggaaccggc gttgctgatc ataacgggag
    42421 gaattatcgg tacgctggtg gtggcgatgt atctgccaat tttccattta ggcgatgcga
    42481 tgagtgggat gggataacgc tggcgtgttg ctccacacca gcgttggaag attacaggtt
    42541 gttgaagatg cggttttctt gttcctgcac acggataaat gtggtgcgct tggtcagttc
    42601 tttcaggcgt gaagccccaa cgtatgtaca agctgaacgc aggccgccca aaatatcacg
    42661 cgcggtattt tcaaccgggc ctcgcagcgg cagcttaacg gttttacctt ctgctgcgcg
    42721 atattccgca acgccgccaa cgtgacgttt catcgcagac tcggagctca tgccgtagaa
    42781 cagcataaat ttctcgccgt tctcctcaac gatgcgaccg ccgctctctt cgtggcccgc
    42841 cagcatgccg ccaagcatga cgaaatcggc accgccgcca aaagctttcg cgacatcgcc
    42901 cggcgtggtg caaccaccat cgctgacgat cattccgccc agaccgtgcg cagcatcggc
    42961 acattcgatt accgcagaaa gttgcggata accgacgcct gttttgacac gagttgtaca
    43021 aacagaacct gggccaatgc caactttaac gatatcggca ccggagagga taagctcctc
    43081 acacatttca ccagtcacta cgttgccagc acaaatggtt ttggtcggcc acgcttcacg
    43141 cgctttcgca acgaactgca cgaagtgttc ggaataacca ttcgccacat caatacaaac
    43201 gaagtttaat gccgggttca ggtcgagaat ctgtttagtt ttttcgaaat ccgcatcaga
    43261 cgtaccggta gaaaccatca catgtttcag cacatcagcg gaagaattgt tgataaacgc
    43321 ttgccactct tcgacagaat agtgtttatg cacagcagtc aaaatatcaa aagaagccag
    43381 cgcagaggcc atagaaaatg tgcctacggt gtccatattt gcggcgataa tcggcacgcc
    43441 ggaccagctc tgacctgaat gtttgaaggt gaattgacgt tccagttcaa catcggaacg
    43501 gcttttaaga gtggagcgtt tagggcggat gagaacgtct ttaaaaccta acttcagatc
    43561 ttcttcaata cgcatgtgcg gattcctggg gttaatggct ataagggtaa gagcgcaact
    43621 ccagtgacgc tatcatacgc actaatcaat gcggcgcaag actgcgaaat tgtctttttt
    43681 tacgctacaa tcccataaat ttacctggta aatagtgagc gaacgtttca tcaatttcgc
    43741 tttcgtgcgc tcatttttaa cgttttgata atcatgatga attcccggga atataaatta
    43801 tgaggtatat agttgcctta acgggaggca ttggcagtgg caagagtacc gttgccaatg
    43861 cgtttgctga tctcggaatt aacgtcattg atgccgatat tattgcgcgt caggtggttg
    43921 aaccaggtgc acctgcgcta catgccattg ctgatcactt tggcgctaac atgattgctg
    43981 ctgatggaac attgcagcgc cgggccttgc gcgagcggat cttcgccaac ccggaagaga
    44041 aaaactggct taacgacctg ctgcatccgc tgattcagca agagacgcaa caccagatcc
    44101 agcaagcaac ctccccctat gtactgtggg ttgtgccatt gctggtagaa aactcactgt
    44161 ataaaaaagc gaatcgagtg ctggtggtgg atgtcagccc agaaacgcaa cttaagcgca
    44221 ccatgcagcg cgatgatgta actcgcgagc atgtcgaaca aatccttgct gctcaggcaa
    44281 cgcgcgaagc ccgccttgcc gtggcagatg acgtcattga taataacggc gcaccggatg
    44341 ctatcgcatc ggatgttgcc cgcctgcacg cacactattt gcagcttgcg tcgcagtttg
    44401 tctcacagga aaaaccgtaa tgcagaccca ggtccttttt gaacatccac taaatgaaaa
    44461 aatgcgtaca tggctgcgca ttgagttttt gattcagcaa ctcaccgtta atttacccat
    44521 cgttgaccac gctggcgcgc tgcatttctt ccgtaatgtc agtgaattac tggatgtttt
    44581 cgagcgcggc gaagtccgca ctgagctgtt gaaagaactt gaccggcagc aacgtaaact
    44641 ccagacctgg attggcgtgc ctggcgtgga ccagagccgt attgaagcat taattcagca
    44701 gttaaaagcg gcggggagcg tattaatctc cgcgccgcgt atcgggcaat ttctgcgtga
    44761 agatcgcttg attgctctgg tgcgtcagcg actgagcatc ccaggcggct gttgcagctt
    44821 tgatttacca acattgcaca tttggctgca tctaccccag gcgcagcgcg acagccaggt
    44881 agaaacctgg attgccagcc tgaacccgct cacccaggca cttaccatgg tgctggattt
    44941 aattcgccag tcggccccct tccgtaaaca aaccagcctg aatggttttt atcaggataa
    45001 cggtggcgat gccgacttgc tgcgcctgaa tctgtcgctc gattcacagc tttatccgca
    45061 aatttccggt cataagagcc gttttgccat tcgttttatg ccgctggaca gtgaaaacgg
    45121 acaggtaccg gaacgtctgg atttcgaact ggcctgttgc taaggagtaa aaatgtcaga
    45181 aactattacg gtgaattgcc caacctgcgg gaaaacggtg gtgtggggtg aaatcagccc
    45241 gtttcggcca ttttgctcca aacgttgtca gctgatcgac ctcggagaat gggctgctga
    45301 agaaaaacga atccccagca gtggcgatct ttccgaaagc gatgactgga gcgaagaacc
    45361 aaagcagtga catttgtctg atgccgcacg taggcctgat aagacgcgga cagcgtcgca
    45421 tcaggcatcg tgtgcaaatg tcggatgcgg cgtgaacgcc ttatccgacc tacaaaaaaa
    45481 aatttatcaa tgagttagaa atccctttag gcctgataag acgcgccagc gtcgcatcag
    45541 gcatcgtgtg caaatgtcgg atgcggcgaa aacgccttat ctgatctaca gacgtttaag
    45601 cttcgcaatt accggttcat tggctggcgg aaaatcatcg gcattaagac cgactagcga
    45661 catccactca ccgggttgcc cttctttacc ccacggctcc ccttcccagc tttcgaccag
    45721 ccagaaccac agagttatat gcctgtccgg gaattcatat tccagttttt caaatagcga
    45781 aaaatgttgt ggggtaatcc cgacttcttc ctgaagttca cgcaccaccg cctgttccgg
    45841 cgtttcaccc atttcaattt taccgccggg aaactccagt ttattcgcca tgtgcgcatc
    45901 tgctgcgcga cgcgttataa agatttcatt gttctcgttg cgaataatac ctaccgcaat
    45961 ttgcagcttt ttcattgtct taaacctata aaaaaggcgc agaatcctgc gccttttact
    46021 tcaactgtta gcttttattg caggcgacca tggcactgct tgtatttttt accggaaccg
    46081 cacgggcaag gatcgttacg tcctactttg cgttcaccgg tttgcgccgc cagtgcagct
    46141 gcggctgcag agtcgtcatc ctgatggcta agctgctgca tttgcgctaa acgctcggct
    46201 tccatacgac gctgttgttc cagctcctca acctcttcag gcatacgtac ctgaactttg
    46261 ctcagcgtac tgataacttc atatttcaac gactccagca tcgctgcaaa catggagaac
    46321 gattcacgtt tgtattcctg cttcggatct ttctgtgcat agccacgcag gtggataccc
    46381 tgacgcagat agtccatcgc tgccaggtgc tctttccaca gggagtcaag cgtttgcagc
    46441 atgacgcctt tctcgaagtg acgcatcatc tcagcaccaa ccacttcttc tttacgctga
    46501 tacacttcga tggactgcgc cagaatgcgc tcacgcagcg tctcttcatg cagttcgggt
    46561 tctttatcca gccactcagc aattggtaaa tcgaggtcga aatcgttctt cagacgttcc
    46621 tgcagccccg gaatatccca catttcttcc agcgactgtg gcggaatgta ggcatcaatg
    46681 gtggctttga acacatcttc acgaatgctg ttgatggttt cgctcacatc gctgacatcc
    46741 agcagttcgt tacgctggga gtaaatggcg cgacgctgat cgttagccac gtcatcatat
    46801 tccagcagtt gcttacgaat gtcgaagtta cgactttcaa ctttacgctg ggcgttggca
    46861 atcgctttag tcacccacgg gtgttcaatg gcttcgcctg gcttcatacc cagtttacgc
    46921 atcattccag atactcggtc ggaagcaaaa atacgcatca gcgcatcttc catcgacagg
    46981 tagaaacggg aagaaccagc atccccctga cgaccagaac gaccgcgcaa ctggttatcg
    47041 atacgacggg attcgtgacg ctcggtaccg ataatatgca ggccacctgc tgccagtacc
    47101 gcatcgtgac gtacctgcca gtcggcttta attttttcaa tttgctctgc ggtcggattt
    47161 tccagcgcgg caacttctgc ctgccagcta ccaccgagca caatatctgt accacgaccc
    47221 gccatgttgg tcgcgatagt caccgcagcc ggataacctg cctgagcaac aatcgccgct
    47281 tcgttggcgt ggaatttggc gttcaggaca ttgtgcttaa taccggcttt ggtcagttcg
    47341 tttgacacca gctccgattt ttcgatggag atagtaccca ccagcaccgg ctggcctttc
    47401 gcagtacgtt ctttgatatc ttcaatgatc gcctgaattt tttccgcttc agtcatgtag
    47461 accaggtcag gcagatcttt acgaatcatt ggacggttgg tcggaacaac gacggtatcc
    47521 agtttgtaga tggagctaaa ttcgaaagct tcggtatcag cagtaccggt catccccgcc
    47581 agtttttcat acagacggaa gtagttctgg aaggtgatcg aagccagcgt ttggttttcg
    47641 ttctggatct gcacaccttc tttcgcttcc acagcctggt gcagaccatc agaccagcga
    47701 cggccctgca tggtacgacc ggtgtgttcg tcaacgatga taacttcacc atctttaacg
    47761 atgtagtcga cgtcacgggt aaacagcgca tgagcgcgca gcgccgccgt tacgtgatgc
    47821 atcagcatga tgttggccgg agagtacaga gactcccctt catccatgat gccctctttc
    47881 accagcagtt cttcaatcag caccagacca cgttcggtca ggttcacctg gcgagatttt
    47941 tcgtccaccg agaagtggcc ttcgccctgg aaggtttcgg agtcttcttt ttcctgacgg
    48001 atcaggtgcg gaataatttt attcacgcgt ttatacattt ccgagctgtc ttctgccggg
    48061 ccggaaatga tcagcggtgt acgcgcttca tcgatcagga tggagtccac ttcgtccacc
    48121 agcgcatagt gcagtttacg ctgtacacgt tcttcagggc tgaacgccat gttgtcgcgc
    48181 aggtagtcaa agccgtattc gttgttcgta ccgtaagtga tgtcagctgc gtaagcttcg
    48241 cgctttgccg gtgctggcat gcccggcagg ttgataccga cagtcaggcc aaggaattca
    48301 aacagcggac ggttgttttc ggcgtcacgt tgcgccaggt agtcgttgac ggtaactaca
    48361 tgcacgcctt taccggttag tgcgttcaga taagcaggca gcgttgcggt cagggtttta
    48421 ccttcaccgg tacgcatttc ggcgatgcag cgttcgttaa gaaccatacc gccgagtaac
    48481 tgaacgtcga agtgacgcat accaaagacg cgcttacttg cctcacgtac cacggcgaaa
    48541 gcttccggga tcagattttc cagcacttcg cctttttcca gacgtgcacg aaactctgcg
    48601 gttttccctt tcagttcttc gtcggagagt ttttccatct ccggttccat ggcattgatg
    48661 atgttgacca ctttgcgcat ccggcgcagg gtgcgatcgt tacgactacc gaaaacttta
    48721 gttaataatt tgattagcat aataaaatct caaacgcccc gcgttgcgga gttaataaaa
    48781 tgaagtaaag gtttattgtt gttagctgag gcgttgaggg ccagcacgga tgccttgcgc
    48841 ctggcttatc cagacgggcg tgctgaattt tgcttgtggg gtaaaatgcg cataatcaat
    48901 gcgataaccc ttttcagacg gcgtgccttc ctgggtcagc agcgcgctga gcgtatccag
    48961 taatgcaaga tgttgcgcct gaagaggcaa agattcttca gcaacgggca gcgtttgcgg
    49021 tgccattgcg aaggaaagat gacggattac cgtgcgaatg gcatgttgat gccagtaatc
    49081 aacggaatag ttcgaattcg ggcggcgcgt gttcgcttcc agcaaggcca attgaccaaa
    49141 gttaactttg gctgaaggct cgtggttgcg ggttgtcgct tttgcgggcg cgtttggttc
    49201 ggcggcgttg ctgagcgcag gcaaacctaa actcgccgca accatcccta ataagagatg
    49261 cggccagaag tagcgtttac caaactgtcg ccagcgcgtc agtattccac tcacgttatt
    49321 gccatcccga tcgtattcaa acgtatttag cgcacaaatc ttatcattaa agcctgaagt
    49381 ccacagcacg aatgaagata agttgtccgt aaaagtgctt aagaaaacag cgttcgcacc
    49441 gttttttata gctcttataa aattcaaaaa aaaacgactg gttcacctgg ccggagagag
    49501 tgccagattt gccagtcgaa ttttatacga cagtataaat gtcgttatgc cagtacagct
    49561 gaaggcgctt tgaaggccaa cggcagttct gcgtcgtcct ggaaggtcac atattcccag
    49621 gcttcctgtt tcgccaggac agcctgcagc agtttgttat tcagtgcatg accggattta
    49681 taagcggtaa atgcaccaat aatattgtga ccacacatga acaagtcacc gatcgcatcg
    49741 agcattttgt gacgcacaaa ttcgtcttca aaacgcaggc cgtcttcgtt cagtacgcga
    49801 taatcgtcaa caacgatggc acaatcgaag ctgccgccca ggcacaaacc acgggactgc
    49861 agatattcga tatcacgcat gaaaccgaac gtacgcgcac ggctgatctg gcgcataaac
    49921 gcatcagcgg agaagttcat cgcatagcgc tggttgctgg aatcaatagc cggatggtta
    49981 aaatcgatgg tgaaatccag cgaaaaacca ttgtacggct taaattcagc ccacttatcg
    50041 ccatcttcga cacgaacagt ctctttgatg cgaacaaatt tcttggcgca gttcaactcg
    50101 tcgataccgg cgtcaagcag caggtataca aacggagcgg cgctgccgtc catgatcggg
    50161 atttccggcg cgttaacttc gataacaatg ttatcgatgc ccaggcccgc gagagcagca
    50221 ttgaggtgct ctacggttga aatccgtaca tcatgctcgt tgaccagaca cgtacagagc
    50281 atggtatcac gcacagattt ggcatcggcc gggaaatcta ccggtggatt caagtcggtg
    50341 cgacgataga tgaccccggt gttggccggc gcagggcgta acgtcagggt gactttcttg
    50401 ccggtatgta aaccgacacc cgtcgcctga acgatacgtt taagtgtcct ttgtttgatc
    50461 atcgtattat ctcgccaaat tacctatcca accgaagtgt actatacatt cggcgggcca
    50521 gtttagcaca aagagcctcg aaacccaaat tccagtcaat tcttaatcag cttgcttacg
    50581 caggaatgct gggatatcca gataatccgg ctctttcgca gtttgcggcg cattgtcatt
    50641 cacgacttta gcaaccggct tctgctcctg ggtcagcgga gccatcccgt gctgctggta
    50701 gcgatccatc actggctgct gaacctgctt attggtcacc agagtgattt caggacgttt
    50761 gtccatgccg atacctgtcg caacaacggt tacgcgcagc tcgtcattca tatccgggtc
    50821 aagagaagta ccgataacca cagtcgcgtt gtcggaagca aatgcacgga tggtgttacc
    50881 taccgtttcg aactcatcca gacgcaggtc gaagcccgcc gtgatgttaa ccagtacgcc
    50941 gcgcgcgcca gacaggtcga tatcttccag cagcggagaa gagatagcca tttcagcagc
    51001 ttcttccgca cggtcttcac cgctcgccac gccagaaccc atcattgcgt agcccatttc
    51061 agacattacg gtgcgtacgt ctgcaaagtc cacgttcatc aaacccggac gagtaatcag
    51121 ttcggcgata ccttgcacag cgcctttgag tacatcgttc gctgcgccaa acgcatccag
    51181 cagggagata ccgcggccca gaactttcag cagtttgtcg ttcgggatag tgatcagaga
    51241 gtccacatgc ttggacagtt cagtgatccc ctgctccgcg aatgccatac gcttcttgcc
    51301 ttcaaagttg aagggcttag tgacgacagc aacggtcagg atacccaaat cttttgccac
    51361 ttcagcgacg actggtgctg cacctgtacc ggtaccacca cccatacccg cagcaataaa
    51421 gaccatgtct gcaccttcca gcgccgcacg cagtgcatcg cgatcctcat cagccgcatt
    51481 gcggccaact tctggattag cgccagcgcc cagtcctttg gtgataccgc taccgatttg
    51541 aatcgtctgt ccaaccgctg ttttacgcag cgcttgtgca tcggtattta ccgcgaagaa
    51601 ttcaacacct tcaatgcgct cgcgcaccat gtgttcaaca gcattaccgc cgccgccgcc
    51661 gacgccgatg actttaatca ccgcgtcatt ggtaagttcc attggttcaa acatagtttc
    51721 tctccgattt gtgcctgtcg cctgaggccg taatcattgt cggcctcata aaaattaaaa
    51781 ctcttttcgc agccaactat tgagtcgctt gatccacgag ccaactgatg ctgtaacacg
    51841 tttttctact tcagcttcac cgttaagatg tgactctttc ccatagtgaa gcaatcccac
    51901 cgccgttgaa taatacggct cctgagcata atccgttaaa ccggtaatgt tcagcggcgc
    51961 gccgatacgc acttgcgtat gaaacacgcg ctgagcacag gctgcaagac cttcgatctg
    52021 cgctgcgcca ccagttaata caatgcctgc cgccaggtga tgtttaaccc cttgttggcg
    52081 aagcttttcc tgcaactgca atatctcttc gttgaccagg ttgagcagct cggtatagcg
    52141 cggctcgatc acctctgcca gggtctgacg ttgcagactc cgcggcggac gaccgcctac
    52201 gctcggcact tccacgctct catcttttcc aacgatggaa cccagcgcac aaccgtggcg
    52261 aactttaatc gcttcggcgt cgcttggcgg cgtgccaaag gcgtaagcga tatcactggt
    52321 cacgacattg ccagcataag gaattacctt agtgtggcgc aatgccccac cggtataaac
    52381 ggcgatatcc attgtaccac caccgatatc gacgacgcag acacccagtt cacgttcatc
    52441 ttccgtcaat accgaataac ttgatgccag accggcaaat atcagttggt caactttcag
    52501 cccacaacgt tcaaccgctt tgacgatgtt tttcgccata tcgttgtggc atgtgatcag
    52561 gtgcactttt gcctgcatcc gcacgcccga aagtcctacc ggattcttga tcccttcctg
    52621 atagtcaatc gcatactctt gcgggatcac atgcagcaca cgatgctcat cgcgcacacg
    52681 caccgatttc gcggtatgga cgacgttttc cacatcttct tgcgtcactt cttcttcaga
    52741 aataggcacc ataccaattt cattctggca gctgatgtgc ttaccagaaa gcgccagata
    52801 taccgaagag atctgacaat ctgccatcaa ttctgcctgg tcaatggcgc gttgtacgca
    52861 cttgaccacg gattcgaggt cgttcacccc gcctttatcc ataccacgcg acgggcagct
    52921 gcccacgcca atgatattga ccataccgtc gggcagaact tcccctacta aagcggcaac
    52981 cttcgcggta ccaatctcca gtcctactac cagttttctg tccgtcgcct tgatcattgt
    53041 tgttctgcct gtgcctgatt ttgttgctga gtagattcct ctggcggcaa gggcgcccag
    53101 cctactgccg ctccagagtc ataacgcaaa tcaacgtagc taatccgttt gccatcggtt
    53161 tgcgcctgct gctgtaaaac cggataaagt tctacaaagc gagccaaacg tttcatcgta
    53221 tcgccccggc caagattgag cttaatatcg ttattcagcg tcaactgcca ggaacgccgc
    53281 gcggtcatcg ccgcttcctt cagagtaaat ctgtcctttg ccagcatctg ccccatttcg
    53341 cgatagccct gcaacacttc attggcgctg ccttccgggc catacagcat tggaagcacc
    53401 tgcttgctgg tgcgatctgg cggcacgctg aaggtatttc cttccgcgtc taccatgtgt
    53461 tgatcattcc accgcgcaat cggcacatat tcaaccagat gaatcttcaa ttcatcaggc
    53521 cactgctttc tgacgctcac ctgcttaatc cacggcaggc gttgttctat ttgcgtctgg
    53581 atgatgttga catcctgggt cataaaggta cccggctcac ccaatgccag gatcgactgc
    53641 cggatatcgt cattacgcgt gtaatggcgt tcaccggtca acaccagctt tgagagcggc
    53701 aggcgttgcg catcttccat ccagcccaac acgacccagc cgctcaccaa cactgtcgtt
    53761 aaaacggtca gcaggaaaag gatccccgcc agacgcgttc cattattgcg gcgagaagaa
    53821 acctcttctt cgctgtttcg cgtgttcaga gcagcctgcg acatattagt ccgccagttc
    53881 cagaattcgt actaccaact gcgagaagct catatctgcc tgacgtgccg ccatcggcac
    53941 caggctgtgg ctggtcatac ccggtgaggt attggcttcc agcagataaa actgtccatc
    54001 actgtccagc ataacgtcaa tacgtcccca tcctttgcaa cctaacgtcg tccatgcttt
    54061 cagcactaat gcctgcaaat tggcctcttg tgacgcttcc agacctgcgg ggcagaaata
    54121 ctgtgtctca tcagagagat acttcgcctc ataatcatag aaggttccgg acggttgaat
    54181 acgtattgac ggtaaaattt cttcaccgag tatcgcaacc gtgaactccg gcccacttag
    54241 ccatttttca atcaatactt cttcatcgtg ctgaaatgcc aatcttaatg catcttgtag
    54301 agcattttct gctactactt ttgacattcc cacactggaa ccttcgcggc tcggcttaac
    54361 gataaccggc aaacccagag cagaaatttc tgctaactgc ttatcgctaa ggcctttttc
    54421 aaactctgcg cgggttaacg ctacccacgg tgcgaccggt aaaccagcac cttgccacag
    54481 cagtttgctg cgtagtttat ccattgaaag cgcagatgcc atcactccgc tgccggtata
    54541 aggcaagccc atcagctcga gcatcccctg cagcgtacca tcttcaccgc cgcggccgtg
    54601 tagcgcgata aacactttct gaaagcccat cgacttcagt tgcgtcacgt cgacttcttt
    54661 cgggtcgaca ggatatgcgt caataccgcc ttcacgcagc ccggctaaca ccgctgcgcc
    54721 agaattcaaa gaaacttccc gctcagcgga ggtcccgccc aacaggaccg cgattttatc
    54781 agtcatgttg ttcttcctcc ggagtttgcg gcttcagttt gatttcagct aaagaacggg
    54841 caatttttcc gatattacca gccccctgaa cgagaatcag gtcgttaccg gttaataccg
    54901 gtgccagcat ctcggctacc ttcgccggat ccggcaccag aatgggatct attttcccac
    54961 gtccacgaat tgtgcgacac agcgaacggc tgtccgctcc cggaattggc gcttcgccag
    55021 ccggatacac ttccagcatc aacagggtat caacctgcgt cagcacattg gcgaaatcat
    55081 catacaggtc gcgcgtacgg gtaaaacggt gcggctgaaa cagcattacc aggtttttat
    55141 ccggccagcc tgcacgcgcc gctttaatag tggcgtccac ttccgtcggg tggtggccgt
    55201 agtcatcgac cagcattgcc gtaccgcttt taccattcac tggctccagc gggaattcac
    55261 caaggaaatc aaaacggcga ccagtcccct ggaagctctc cagcgcacgc aaaatagcct
    55321 cgtcgtcaat gccctcttcc gtagcaaccg caaccgcagc tgcggcgttc agcgcgttgt
    55381 gacgacctgg tgcattcagg gtgacgcgca tcggctcttt gtcctggcgc agcagcgtaa
    55441 agtgcccctg cgggccaatc tgctgataat cttctacacg cacgtcggca tcttcgctga
    55501 agccgtaagt tgtggtctga cgtcccactc gcggtaacaa ttcgcggatc accggatcat
    55561 caacacacat caccgcacga ccgtaaaacg gcaggttgtg cagaaaatta ataaaagtct
    55621 gttttaaatt ctcaaagtcg ccctggtagg tatccatgtg gtcggcttcg atattggtga
    55681 caatcgccac catcggttgc agatgcagga acgatgcatc actctcatct gcttcggcaa
    55741 tcaggtaccg accatgcccc aaacgcgcat gaacccccgc cgcttttacc agcccgccgt
    55801 taacgaaggt tgggtcgagc cccgcttctg cgtagatgct ggagaccatc gcggtagttg
    55861 tcgttttgcc gtgcgttcct gcaatggcga tgccatgacg aaaacgcatt aactcagcca
    55921 gcatttcggc acgacggatc accggaatac gcgcttcatg agctgcgaca atttccgggt
    55981 tatcggcaga aatagcgctg gaaacaacga ccacgctggc atcacgtacg ttttccgggc
    56041 gatggttgaa ataaatcgtc gctcccagat tcattaactg ctgcgtgacc ggatttggcg
    56101 ctaaatcgga accactgatc tgataacctt cattggccag aacttcggca ataccgccca
    56161 taccggcacc accgatgccg acaaaatgta tgtgccgaac gcgacgcatt tcgggcacga
    56221 tggaacgcag ttttgccaat tgttgtgtat tcattcttta cgccattaac ttcttaaatt
    56281 catacgatgc aaaaggcatc gctacaatta cgcccgggca gcccggctca cttcatttgc
    56341 cactcgctcg gtggcatccg gaatggatgc agcgcgggcg cgttctgcca tggttaataa
    56401 ggtttctcgc gaccacccgg ccagggtgtt ggcgacagca tccacgctaa gctgtggctg
    56461 ctcgataatt ttggctgcgc ccgctttttc cagcggtagc gcattccagt attgctggcg
    56521 gtctttatgt tgaaacggca caaacaacgc cggtagtcct gccgcggcga tttcactcac
    56581 cgttaaagca ccggagcggc aaacgacgac atccgcccac gcatacgccg ccgccatatc
    56641 atcaataaat tcagtcactt tatgctgcgg ttgccccgct tcggcatacg cctgttcaac
    56701 ggattgttgc gaacctttgc cgctctgatg ccagatagtg actgaatcac ccagtttcgc
    56761 agcaacctgc ggcattgtct ggttaagaat gcgtgcgccc tgggaaccac ccactaccag
    56821 cacacgaacc ggaccttcac gtccagccaa acgttgctgc ggcaacggta gcgccaacac
    56881 atcggtacgc accgggttac ccaccacttc cgcattgggg aaagcacctg gaaacgcctg
    56941 catcactttg gtggcaatct tcgccagcca tttattggtt aagcccgcaa taccgttttg
    57001 ttcatgaagt acaaccggaa tgcctaacga ccacgcggcc agaccaccag gacctgatac
    57061 gtagccgccc ataccgagca ccacgtcagg tttgtacgct ttcataatcg cccgcgcctg
    57121 acgccaggca ttgaagatac gcagcggcgc agctatcagt gcttttatac cttttccacg
    57181 cagaccagag atacgaatga aatcaatttc gatgccatgt tttggcacta agtccgcttc
    57241 catacggtcg gcagtcccca gccagcgaac ttgccaaccc tgagccatta gatggtgcgc
    57301 aaccgccagt cccgggaata catgtccacc ggttccgcct gccatcacca ttaatcgctt
    57361 tccttgacca ctcatcgtga acctcgtaca aacgcctgcg ctttctccag acgcgtttca
    57421 taatcaatac gcaacagcat catgatggct gtcgacataa tcagtaagct cgaaccaccg
    57481 taactgatca gcggcaatgt cagacctttg gtcggtaaca tccccgccgc cgcgcctacg
    57541 ttaaccagcg cctggaagct aaaccagatg ccaatagaac aggcgagaaa accggaaaaa
    57601 cggtggtcaa tttctaatgc tttacggcca atcgacatcg cgcgaaaagc gacgaagaat
    57661 accattaaaa gtgccagcac cacaccgaca taccccagtt cttcgccgat aatggcgaaa
    57721 ataaagtcag tgtgcgcttc cggcagatac tccagttttt gtaccgagtt acctaaacct
    57781 tgtccccaca gttcgccgcg accaaacgcc atcagcgatt gcgttaactg atagccgctg
    57841 ccaaagggat cttcccacgg gttccagaat gcggtaacac ggcggatacg gtacggttcg
    57901 gcgaggatca gcaacacaac cgctgaaatg cccataccga taatggcaat gaactgccac
    57961 aatttcgctc ccgccaggaa caacatcgcc agcgtagtca caaacaacac caccaccgta
    58021 ccaaggtctg gctgtgccag cagtaacact gccaacacca gaatcacgcc catcggtttc
    58081 aggaagccgc gcaggttatt acgtacttcg tcgcctttac gcaccagata gttggcgata
    58141 tagcaaaaca gcgacagttt tgtcagctcc gcaggctgga tacgcagcaa accgagatcg
    58201 atccaacgcg atgccccttt aaccgagctc cccactacca gcacgatcat cagcaggatg
    58261 atagatccga gcagcatcgt ggcactgtag cgttgccaga actccatcgg cagacgcagc
    58321 gtaatgatcg ccagaataaa cgccaaaatc agatagacac catcacgctt cgcgaagaag
    58381 aacggatcgt tggttaagcg ttgccctatg ggcattgacg ccgaggtcac catgataaag
    58441 ccaatcgccg cgaggccgaa ggtcagccac agtaaggtgc gatcgtacat gatcaggctg
    58501 tcggtatctt tttcccgcga gcccatcacc cagcccttta gcgccgtgga gatccagacc
    58561 aggatactga atcctggcag gcgcggcatt ttcaggcgag ggagagataa acgcatcaac
    58621 ctaactcctt cgccagacgg gcaaactcat tgcctcgttg ttcaaagttc ttgaactgat
    58681 caaggctggc acaggccggg gagagtagaa ccatatcgcc cggctgaaca cgcggagcca
    58741 gcaagcgcat cgcctgttcc atagtttcgg tttgttctgc cacttccggg cgtagcgccg
    58801 ccagctgcac gccgtcacga ccgaaacaat acagacgtac gttatcgcca ttcaggtaac
    58861 gcgccagtgg gctaaagtcc gccgatttac catcgccacc cagcaacaaa tgcagcgtgc
    58921 cgtctacgtg caggccattc agcgccgctt ccgtactgcc gacgttggtc gctttcgaat
    58981 cgttaatcca acgtacgccg ttatgctcca gcacaacttc aaagcgatgc ggcagaccag
    59041 tgaatgtggt taacgctttc aggctgctgg ctcgcggtaa ccctgcggca tctgccagtg
    59101 ccagcgccgc cagcgcattg gtgtagttat gctgcccgga aattttcatt tctttcacgt
    59161 tcagcacttt ctcgcctttc acccgcagcc aggtttcgcc ctgctgatga ttcaggtgat
    59221 agtcacccat gttgacgcca aagctgacgc agcgtccatc cgcaccgcga atcggcattg
    59281 ttaaggcatc atcagcatta accacgcaaa ctttcgcgtt ttcgtaaatg cgcagttttg
    59341 ctgcacgata ctgttgtaaa ccaaacggat agcgatccat atgatcttca gtcacgttca
    59401 gaatggtcgc tgctaccgcc tgtaagctgg aggtggtttc cagctggaag ctcgacagtt
    59461 ccagcacgta cagttcacac tcatcatcca gtagcatcaa cgcaggcagg ccaatattgc
    59521 cacccacacc aacgttaacc cccgccgctt tcgccatttc acccactagc gtggtgaccg
    59581 tgcttttgcc gttagaaccg gtaatcgcca caatcggtgc ttgtgcttcg cgacagaaca
    59641 gctcgatatc gccaacgatt tcgattccgg catcagcggc agcgcttaag gatggatgcg
    59701 ccagtgcaat accgggactg gcgacaatca gatctgccgc catcagccat tcatcattca
    59761 gactgcccgt gtggcgttca acggcttcgg gtaatttatc caggccaggc ggtgtcatac
    59821 gcgtatccat aacgcgcggc gtcacaccgc gagcgaggaa aaagtccacg caggaaagcc
    59881 cggtgaggcc caggccgata atgacaacat ttttaccctg gtaatcagcc atgattaacg
    59941 taccttcagc gttgccagac caatcaggac cagcatcagc gaaataatcc agaaacgcac
    60001 aatgacgcgc ggttccggcc agcctttcag ttcatagtgg tgatgaatcg gtgccatgcg
    60061 gaaaatacgt tgtccgcgca gtttaaagga gccgacctgc aggatgacag aaagcgtttc
    60121 taccacgaac acgcccccca taatcaccag caggaattcc tgacgtagca gtacggcgat
    60181 aatgcctaac gcaccaccta acgccagcga acctacatcg cccataaaga cctgcgccgg
    60241 ataggtgtta aaccacagga agcccagtcc tgccccgact atcgcggtac agacaataac
    60301 cagttccccg gcgtgtcgca gatacggtat gtgtaaatag ctggcaaagt tcatgttgcc
    60361 ggtcgcccac gccaccagcg caaaaccacc ggcgacaaat acggtcggca taattgccag
    60421 accgtcaaga ccatcggtca ggtttaccgc gttgccagta cccacaatga cgaagtaagc
    60481 cagcaaaatg tagaacagcc ccagttgcgg catcacatct ttaaagaatg ggaccaccag
    60541 ctgcgttgcg ggcgtgtctt tgccaacaag gtacagcgcg aaggcgacac ccagcgcaat
    60601 gaccgacatc cagaaatact tccaacgagc gatcaaccct ttggtgtctt tacgcactac
    60661 tttgcgataa tcatcaacaa agccaataac accgtaacct accagcacca ccaacacgca
    60721 ccagacgtac ggattggacg ggtaagccca cagcagtacg gagatcacaa tcgccgtcag
    60781 gatcataatc ccgcccatgg tcggcgtacc gcgcttgctg aagtgtgatt caggaccgtc
    60841 gttacgcacc acctgaccaa aggaaagttt ttgcaaatga gcaatcatac gcgggcccat
    60901 ccacaatgag atgaacagcg cggtcagcag gctgacgatg gcgcgaaacg tcagatagga
    60961 aaagacgtta aagccggaat aatatttgac caaatgttcg gccagccaaa ctaacatgtc
    61021 ccattctcct gtaaagcgcg tactacctct tccatggcgg cactacgtga acccttaact
    61081 aaaatcgtaa ttacctgttg ctcagcaatc agtgatttaa gacgcgtaat taacgcagtt
    61141 ttatcagcaa aatgttcgcc aacgccgctg gcggtgctga tagcatggct ttgtttaccc
    61201 acgcttaaca cgcggtcaat accagcagct tttgccgcct cgcccacctg tacatggcag
    61261 gcttcgcttt cagcgcccag ttccgccata tcgcccacca ccagcacgcg gtagcccggc
    61321 atttcagcca gtacctggac tgctgcggtc attgaaccga cattggcgtt gtaggagtcg
    61381 tcgagcagca actggttttc tgccagttgg atggggaaca gacggcctgg aacagctttc
    61441 agatttgcca gccccgcttt gatagcatca agcgttgcgc ccacggacat ggagagcgca
    61501 gcggctgcca gcgcattcgc aatattgtga cgccccggca acggcagcag aacatccacg
    61561 ctaccggttg gggtttgcag ggtaaattcc gtaccgtgcg aagtcacatg gatattggtg
    61621 gcggtgaaat cgctgttggc ggcattaggt gagaaacgcc acactttgcg tgagccaatt
    61681 acgctctgcc agttcagcca gtcgttgttg tcggcgttca taatggcgat accgttttcc
    61741 ggcaggccgc taaagatttc acctttcgct ttcgcgacac ccgcaagcga gccaaaacct
    61801 tccagatgcg ccgctgccag gttgttgacc agcgcagctt ccgggcgagt cagactcaca
    61861 gtccaggcaa tttcgccctg atggttcgcg ccaagttcaa taactgcgta atcgtattcc
    61921 ggcgttaagc gcaacagcgt catcggtaca ccgatgtcgt tgttgagatt gcctgccgta
    61981 taaagcgtgt tgccgcactg gcttaaaatc gccgccgtca tctctttaac ggaggttttg
    62041 ccggaggacc ccgtcagagc aaccacgcgc gccggaactt gctggcgaac ccatgcagcc
    62101 agttcaccaa acgccagacg cgtatccttg acgattaact gcggcaggtc gatgtccagc
    62161 ggacggctaa ccagtagtgc gcccgcgccg ccagctttcg cctggtcggc aaaatcatga
    62221 gcatcgaaac gttcgccttt cagggcaaca aacaggcagc ccggcgtcag ttttcgggta
    62281 tcagtggtta cagcatcaaa ggtgatatct gcaccttgca gttcaccgtt gagaatgtcg
    62341 gtaagttggc taagggttac gctaatcatg caatcacccc cagcagacgc gccaccgtga
    62401 cgcgatcgga gtagtccaga cgctgattgc caacaatctg gtaatcttca tggcctttgc
    62461 ccgcgaccag caccacatcg ttctctttag cctgcataac ggcgcaagtc accgcttcag
    62521 cacggccttc catcactttg gcatgtccgg catctaacat tcccgccaga atatcgttga
    62581 tgatggcacg cggctcttcg gtacgcgggt tatcgtccgt caccaccgcc acatcagcaa
    62641 actcttcggc aattgcgccc atcagtggac gcttaccttt atcgcgatcg ccaccacagc
    62701 caaagacaca ccacagcttg cccgcacagt gcagacgcgc cgcctgtaag gctttttcca
    62761 gtgcatccgg cgtatgcgcg taatccacca ccaccgtcgg tttgcctggc gcagtgaaca
    62821 cttccatacg tccgcaaacc ggttgcagac gcgcggcggt tttcaacaga tccgccaatg
    62881 gatagcccag tgccaacagc gtcgccagcg cgagcagcag gttgctgacg ttaaaagcgc
    62941 ccatcagatg gctctcaatt tcgccatcgc cccaacttga gctaaagcga atcgtcgcac
    63001 cgctgtcgtg atagttcacg tcgttcgctt ttaaccagcg tccgtgacag ttcggattga
    63061 tatgatcttc cattgatacc gcaaccgcgt ccggcagttt tgccagccag cggcggccca
    63121 cttcatcgtc ggcgttaata atcgcctgac cgcaatgatg ctcagaataa agcagccatt
    63181 tcgcggcttc gtagtgttcc atatcaccat gataatcaag gtgatcgcgg cttaagttgg
    63241 taaagaccga cgccgcaaat ttcaatgccg ccacacggtg ctgtaccagc ccgtgggagg
    63301 aaacttccat tgcgcaaaac gtcgcgccct gatccaccag ccccgccagc tcgtgctgaa
    63361 catcgactgc cgaaccggta gtattttctg tcgggatcac tttccccagc agaccgttac
    63421 caacggtacc cattaccgcg ctggtttcgc caagcagttg gctccactgc gccagcagct
    63481 gggtagtcgt ggttttgccg ttggtgcccg ttacgcctac gagacgtaaa ttgtcagagg
    63541 gttcatggta aaagcggccc gccagtgcag ataaacgctc attgagctgg ctgagataga
    63601 tgaccggtac gccgtgcatt tcacggattt caccgtcggt cgcctcatct ttcgcctctg
    63661 caataatggc agccacacct tgcgctatcg cctgcgggat atatcgacgc ccgtccgcct
    63721 gatgacctac tacagctaca aagagatcgc ccgccgcagc cacacggctg tcgagtgtca
    63781 tctctcgcag tgctcgcgaa ggtgcgtctg gcacccacgg agcaagaagg tcgcgcaaat
    63841 tacgatctgc cacctgtccc ctcgccttga ttaatcacaa attcattttt atcgcccgtt
    63901 gtcagcgcat ccggctcgat gttcatggta cgcaatacgc cgcccatgat ggcaccaaag
    63961 accggcgcgg aaacggcgcc gccgtagtat ttacccgcct gcggatcgtt gataacaaca
    64021 accagcgcga agcgcggctg actcgcaggc gcaacgcctg cggtataagc aatatattta
    64081 ttgatgtagc gaccgtccgg cccgaccttt ttcgcggtac cggttttaat ggcgatacga
    64141 tagcctttaa tcgccgcctt cacgccgccg ccgcccggta gcgccacgct ttccatcata
    64201 tgcaccacag tgcggacaat ggattccggg aagacacgtt caccgggaac cggggggtca
    64261 actttggtaa tcgacagcgg gcgataaatg ccgtagctgc cgatagttgc gtagactcgc
    64321 gctaactgta acggtgttac cattagcccg tagccgaaag agaaggtggc cctctctatg
    64381 tcagaccacc gttgtttttg aggatataag ccactgcgtt ctccgaccaa ccccaaattg
    64441 gtcgcttttc ccagtccaaa acgtgagtaa gtatctacta acgctgagga cggcatcgct
    64501 aacgccagct tggaaacacc gacgttactc gacttctgta ataccccggt cagggttaat
    64561 tcgctgtagc gtgccacgtc tttgatttcg tggccgttaa ttcgataagg aatggtattg
    64621 agtaccgagt tttcccgcac cacgccacgt tgcaacgcgg tcatcaccac catcggttta
    64681 accgttgagc ccggttcaaa cacgtcggtg atggtacggt tacgcatcgc ctcttttggc
    64741 gtgccgctca gattatttgg gttgtatgat gggctgttag ccatcgccag cacttcaccg
    64801 gtgttgacat ccaccagcac ggcgctaccg gactcagcct tgttaaaggc caccgcgtta
    64861 ttcagttcgc gataaaccag cgcctgcagg cgttcatcaa tactcagcgc caggttgtgc
    64921 gcagcctggc tgtcagtaga agaaatatct tcaattacgc gaccatagcg gtctttacgc
    64981 acaatgcgct cacccggctg cccggtaagc catttatcga aactcttctc gacgccctca
    65041 atcccctgac tatcgacgtt agtaaagccg atgaggtgag cagtcacttc gccggatgga
    65101 taataacggc gagattcttc acgcagatga atccccggca gtttcagttt tttgatgtag
    65161 tccgccatgt cagggtttac ctgacgcgcc agataaataa agcgcccttt cgggttggcg
    65221 ttaatgcggg ctgaaagctg atccagcgga atattgagcg cgttagccag cgccttccag
    65281 cggtcaccga cgctgatacc gccagcgtca tgcacttctt tcgggtcagc ccaaatcgct
    65341 tttaccggca cgctcaccgc taacgggcga ccagaacggt cagtaatcat gccgcgggag
    65401 gtggaaactt gctgaacgcg aagagaacgc atgtcgccct ctttcaccag catatccggg
    65461 gagataactt gtaaccacgc tacgcgtccg agcagaaaag ccagcgccag gagaatacag
    65521 ccgcataaca acgcaaaacg ccaactgata aagttggcat gttcttcctg acgttttggt
    65581 ttctgcgttt tcgccgctgc tttcatgcgt cgcgtttatc cttatttttg cactacgata
    65641 ttttcttgtg acggatcaac atgctgcatt tgcagctttt ccgtggcgat cctttccacc
    65701 cggctatggt cgccgagcgc attctcttca aggatcaggt tgcgccattc aatgtctaaa
    65761 gcatctcgct ccagcaccag ttgttcgcgc tgagcggtca gtaaacgggt atggtgcgcc
    65821 gtggttacca cggtcaccgc cgtcaaaata atgcaaatga acaggcagag tggcagcttc
    65881 ccaaatcgca aaagatcgtc accgataaca ccaggcaatg catggcgctc gtggcttccc
    65941 atcgatcctt taactttgct tagagcttct gtcactctgc tgatcatgcg ttcgtcctct
    66001 ctgcaatacg cagaactgaa ctacgggcac gaggattctc agccacctct tcttcgcccg
    66061 gcattaactt gcctagtgct cgcagctgac ggccacccag ttttttgagc tgctcttcag
    66121 tcatcggtaa ccctgccgga acttgcggac cgcggctgtt ttcacgcata aaacgtttca
    66181 caatacggtc ttccagcgag tggaagctga tgatcgaaag ccgcccaccc ggtgccagca
    66241 cgttgagcga gctttttagc gcctgctcta tctcctccag ttcactgttt acccaaatgc
    66301 gcaccgcctg gaaggtacgg gtcgcgggat gtttaaactt atctttcacc ggcgttgcag
    66361 cagccacgac ttccgccagt tctttggtgc gggtcatcgg ctgttcgcgg ttacgctcga
    66421 caatggcgcg ggcaatgcgt ttggcaaaac gctcttcacc ataggttttc aatacccagg
    66481 cgatatcggc ttcttctgcg gtttgtagcc attcagcggc tgactgccca cgggttgggt
    66541 ccatacgcat gtccagcgga ccatcgcgca taaaggaaaa gccgcgttca gcatcatcaa
    66601 gttgcggtga agagacgcca agatcgagga gaatgccgtc gatcttgccg ataagatcgc
    66661 gctcggcaac gtattcgccc agcgcggaaa aaggtccgtg gatgatggag aagcgcggat
    66721 catcaatagt cttcgcaacg gcgatagcct gcgggtcgcg atcgatcgcc agcaaacgcc
    66781 cctcttcgcc aagctgcgag aggatcagac gtgagtgacc accgcgacca aaagtcccat
    66841 caatgtagat gccatcagga cggatattga ggccattaac ggcttcatcc aacagcaccg
    66901 tagtatgttt atagttttcc atcattttat agagacaagt cctgcagtcg ctccgataag
    66961 tctccggtag ccaactgctc tgcgtcgata tcttccttga cctgttgatg ccaggttgtt
    67021 tcatcccaca gctcaaactt gttgaactgt ccaaccagca tcacttcttt tgtcagcccg
    67081 gcatgttgcc gcagtactgg cgcgattaac aatcgacctg cgccatccat ctgacattcg
    67141 ctggcatgac ctaacagtag gcgctgcaca cggcgctcaa ccgggttcat gctcgacaga
    67201 cgcgataatt tttgctcgat aatttcccat tcaggcaggg ggtaaagcag caggcacggg
    67261 tgatgaatgt caatggtgca aaccatttga ccggcagcgt tctcaagcag ctgttcccga
    67321 taacgggtag gcactgataa gcgccctttg ctgtcgagat tgactaacgt tgctccccgg
    67381 aacatgccag cctcacccct tattcccact ttgccccaca atttcccact gaaaggagtt
    67441 tacggagcgg aggaaaagct tgtcaagcca gtcacaaggc ataccgaggt aaaaaacccg
    67501 atgtttacgg ctaatatcag cgttgtttat tttacgtcca acgtgcgaag caaattaacg
    67561 ttatgaatag ctgtaagaaa aaaaccctca tcgtcatcac cgttttaaaa tgactcacta
    67621 ttttccacag caaatataaa gtgtcagttt gcgacgcgag cggcatttta ggacatatct
    67681 tccccggtta acagtccctg ttgcgtctgt gtagcgcttg atgccagcgg gtttgcgcaa
    67741 aagcaaagaa aagtgcttgt taaatcggca attcatctct gcatgtaaca aaataatgca
    67801 aaaacgcgct gtcgatatca cttaattatt gtgaatttca gctggtgagt taattaacgg
    67861 gcgggtttaa aagcatttat caataatagt caggaatagt cttatttact ttaaggatat
    67921 tgatgtccag tcccgtactc tacgcgccag agtgaaattc acctggcgcg tatttttgtt
    67981 cgcagcttag ctacggctga gcacaccgcg gcgatagaga ttacgtttaa tgcgcgttaa
    68041 accaggtttt ggcttacgcg gttcgtccag gcttgccagg acaatctcca gcacacgctc
    68101 tgcgacatcg cggtgacgtt gagccactgc cagcaccggg cactgtaaga agtcgagcag
    68161 ttcgttatcg ccaaaggtgg caattgccag gtcagaaggc agtttaccat cgcgacgtag
    68221 cgtgacatcc atcacccctt gcaacaacgc aaacgacgtt gtgaacagcg cctgcggcat
    68281 cggatgcgtt tccagccatt tttcgaataa ctgggcagcc gcctcccgct catagctgtt
    68341 ggcatacaga aaatgcactt cgcgcggatc atctttccag gcagtacgga aaccttgttc
    68401 acgcaggaag ctgacagaaa gctccggcag cgcgccaaga taaagcaccg tctcggcggg
    68461 aaacttacgt aactcttccg ccagcatttc ggcatcatcc tgatcggcac caaccacgct
    68521 ggtaaagtgt tcacgatcga gggcgcggtc cagcgcgaca atcgggaacg ggtcgttagc
    68581 ccagcgttga taaaaaggat gctcaggagg caacgacgtc gaaacaataa tggcatcgac
    68641 ctgacgctgt aaaaggtgct caatacagcg catttcgttg tctggctgat cttctgagca
    68701 ggcaatcagc agttgatacc cccgttgccg cgcctggcgt tcaagatagt tagcgatgcg
    68761 ggtatagctg gtgttctcca gatcggggat cacaagacca atagaacgtg tgcgtccagc
    68821 acgaagccca gctgccacgg cgttcgggtg gtaattgtgc tcacgcacga cagccatgac
    68881 tttttcaacg gttttgtcgc tcacacggta ttgcttcgct ttgccgttaa taacatagct
    68941 tgcagtggtc cgcgacactc ccgccagccg agcgatttca tccagtttca caattgcccc
    69001 ttgcgtaaaa tgtaaaaacc atagccattg tactggtatg ggttaaattc attaacatct
    69061 aagcgcagaa aattaactgc ggcaactgct tttattccag gttcccactg atttcgcaaa
    69121 aaaagcccaa cgtgacatgt tgggctgtaa attgcgcatt gagatcattc tcaacgcatt
    69181 attttatcgc cgcgcgaaag tccgaccaca ccagagcgag caacttccac aattttcgcc
    69241 acatcgcgaa tcgatgctaa aaatgcatca agcttatcgc tggtgcctgc taattgaacg
    69301 gtataaagcg agggtgtgac atcgataatt tgcccacgga atatttccgt attacgtttc
    69361 acttcgtcac gcccgtaacc gctggcctga attttcacca gcatgatttc cctctcaaca
    69421 tgcgcgccct gccccaactc actcacgcgc aagacatcga ccagcttgtg taattgcttt
    69481 tcgatctgct caagtacttt ttcatcgccc acggtctgga tggtcatacg cgataatgtc
    69541 ggatcgtcgg ttggcgcaac ggtcaggctt tcaatgttgt agccacgctg ggaaaaaagg
    69601 ccaatcacgc gggataacgc gcctgattca ttttcgagta agactgataa tatccggcgc
    69661 ataatcaggt tctctccgtt ttgcttaacc acatttcatc cattccgccc ccgcgaatct
    69721 gcatcgggta gacgtgctcg ctgccatcga cggtaacatc aacaaacacc aggcgattac
    69781 tgcgcacctg ttccagcgcc tcgctaagtt tgctttccag ctcatgcgga tgagaaatct
    69841 ggatcccgac atgcccatag gcttccgcca gacggacgaa atcgggtagc gattgcatat
    69901 aagattgtga atgacggccg gaatagatca tgtcctgcca ctgcttcacc atccccagat
    69961 agcggttatt gagattcacc accagtacgg gcaactcgta ttgcaacgcg gtagacagtt
    70021 cctgaatgtt catctgaata ctgccgtcgc cagtgacgca aaccacggtt tcttctggca
    70081 acgccatttt gacgcccagt gccgcaggta aaccaaaacc catcgtgccg aggccaccgg
    70141 aattgatcca gcgacgcggt ttgtcgaatg gataataaag tgcagcaaac atctggtgct
    70201 gcccgacatc ggacgtcacg taagcgtctc ccttcgtcaa ccgccaaaga gtctcgatca
    70261 ccgcctgcgg tttaatcttt tcactgtgag tgtcatattt caggcactga cgagcgcgcc
    70321 actgttcaat ttgctgccac cagtcgcgga tctcatccag tggttgatgg gcggattctt
    70381 gcgacaagag ttcaagcatt tgttcgagga cctggcgagc atcccccaca atcgggatat
    70441 ccgcagtcac ggttttagaa atggaagtag gatcaatatc gatatgcaga acagtggcat
    70501 ttgggcagta ctttgccaga ttgttcgtcg ttcggtcatc aaatcgtacc ccgacggcga
    70561 aaatcacatc cgcgttatgc atcgtcatat tggcttcgta ggtaccgtgc attcccagca
    70621 tgcccagtgc ctgacgatgc gttgccggaa acgcccccag ccccatcaat gaggaaacaa
    70681 cgggcagatt caacgcctcc accgtttctt tcaactgctg atggcagccc gccgtgattg
    70741 ccccaccgcc tacgtagaca accggttttt ttgccgctac cagcgtttgc agagcacgct
    70801 taatttgccc tttatgtccg gtagtagtgg gattgtaaga acgcatactg accgactccg
    70861 gccagacata gggtaatttg ttcgccggat taagaatatc tttcggtaaa tcaacgacca
    70921 ctggtcctgg gcgaccactt gccgccagcc agaaagcctt tttcagcacc tgcggaatgt
    70981 cttccgtttg cttaaccaga aaactgtgtt taaccaccgg tcgcgaaatc cccaccatgt
    71041 cgcactcctg aaaggcatcg taacctatca acgaggtcgc tacctgcccg gaaaggacaa
    71101 ctaatggaat ggaatccata taagcggtgg cgatgccagt aatcgcattg gtcgcccctg
    71161 gacccgacgt taccagcacg acgccgactt ccccggtcgc gcgcgccaga ccatcggcca
    71221 tatgcactgc cgcctgctca tgacgaacta aaacatgatc gataccaccc acggtatgca
    71281 atgcatcgta aatatcaagg actgcgcctc cgggataacc gaatacttgt ttaacgccct
    71341 gatcgataag cgatcggacg accatctcgg ctccagacaa catctccatg gcctgcctca
    71401 ctgtttgacg gaaaaaatgt gtaaaacaca ttaaccgtcc gatcttgctt aagcaattgg
    71461 cagccagaga cataaacgcc gggtaataag aataaacagg aggaaaggtg aaaagaaacg
    71521 ataaaatcct ccattacgaa ggataaaaat gataataaat cggctaaatc ccaccactta
    71581 cagcaatttc tctatttttt taattaatcc taaaatagag agcccacata ttcagaataa
    71641 aattctacat tcaataaaaa atgcagaata aaccagacat tcatgtctga cctattctgc
    71701 attcagttag cgtttgcaaa ttgagactaa ttgctcttcc atccactgat ggcctttatc
    71761 gcgcccggca gcttcatgcc aggagagata acaggttctg ctatttagtt ttaacggcag
    71821 cggtaatacc tgtaattcta aggattcagc gaactcttca gccagccaac gcggcgcaat
    71881 agcgaccaaa tgcgtttgcg acaccacgct aagtacgctc atcattgcca tgccctgata
    71941 cgcgatactg gcttgcttat ctaccgtgtc ataccacggt tgactaaatg acgcgaaacg
    72001 atcgagcgaa accgccgcat gttgttcgtt ataaacatca tgtttcagta acgggccctt
    72061 aattgttggg tgatttttgc tggctaccag caccatttca tctttaaata atggcacgct
    72121 ggtaaattca ggacgatgga agtcttcata actaatcaca aactccgttt cctgataacg
    72181 cagctgatgt tcagtgttct gatttaatga agacttgaac ataacatgta tatttggcgc
    72241 aatctgctca atgtgattat aaatctgcga ggtcagaata ctatctaacg ggctgcaaac
    72301 acaaagatga aatacacgtt cactgctcgc gggttcaaaa cctgaaccag gcaattcatt
    72361 ttgtactagt tgcaatgcct gacgaactga accaaaaagt tgaaatgcgc gagcagtcgg
    72421 ttgaatacca cggccataac gaacaaaaag ctcgtcatta aacatcacct tcaggcgtgc
    72481 aacagcgtta ctgaccgcag gttgcgacat tcccagaaca tgagcagcac gggtaatgtt
    72541 ttgctcctgc atcacggcat cgaaaacggt taataagttg agatcgacca tgcgtagctg
    72601 tggcttgctg aactccgcca tctctggatg atctgtttgt acctctggca tacttaactc
    72661 cactgtcaca cttaactccc tttcccttat tggaatgcag agaaataatc ctcgagaata
    72721 tgatttatca tgcatataaa ataagaaaaa ggaaaatgag taaaattagg aaaacataaa
    72781 gatacgggtt cttataaaaa cagaatccat tgccgtggaa agcgtcactt accacagcat
    72841 aataatccat aatgtttttg ttgcgaaaac aatctaatca taactaccgc gaatactcaa
    72901 tcatccacaa aatggattaa atgtgctttt taataattta tttatatgtt taat
//
LOCUS       DAZFKF010000014        69840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFKF010000014 DAZFKF010000000
VERSION     DAZFKF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.014"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..288)
                     /locus_tag="ACHKA1_001126"
                     /pseudo
     CDS             complement(1..288)
                     /locus_tag="ACHKA1_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            561..1355
                     /gene="yjfZ"
                     /locus_tag="ACHKA1_001127"
     CDS             561..1355
                     /gene="yjfZ"
                     /locus_tag="ACHKA1_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO5678813.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLAKKCNAYDGVTMNGDNSFGWNHETTLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
     gene            complement(1426..1875)
                     /gene="rplI"
                     /locus_tag="ACHKA1_001128"
     CDS             complement(1426..1875)
                     /gene="rplI"
                     /locus_tag="ACHKA1_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO5678814.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1917..2144)
                     /gene="rpsR"
                     /locus_tag="ACHKA1_001129"
     CDS             complement(1917..2144)
                     /gene="rpsR"
                     /locus_tag="ACHKA1_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO5678815.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(2149..2463)
                     /gene="priB"
                     /locus_tag="ACHKA1_001130"
     CDS             complement(2149..2463)
                     /gene="priB"
                     /locus_tag="ACHKA1_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO5678816.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(2470..2865)
                     /gene="rpsF"
                     /locus_tag="ACHKA1_001131"
     CDS             complement(2470..2865)
                     /gene="rpsF"
                     /locus_tag="ACHKA1_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO5678817.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            3192..3467
                     /gene="yjfY"
                     /locus_tag="ACHKA1_001132"
     CDS             3192..3467
                     /gene="yjfY"
                     /locus_tag="ACHKA1_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO5678818.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(3596..4282)
                     /gene="ulaF"
                     /locus_tag="ACHKA1_001133"
     CDS             complement(3596..4282)
                     /gene="ulaF"
                     /locus_tag="ACHKA1_001133"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO5678819.1"
                     /translation="MQKLKQQVFEANMELPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4282..5136)
                     /gene="ulaE"
                     /locus_tag="ACHKA1_001134"
     CDS             complement(4282..5136)
                     /gene="ulaE"
                     /locus_tag="ACHKA1_001134"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO5678820.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(5146..5796)
                     /gene="ulaD"
                     /locus_tag="ACHKA1_001135"
     CDS             complement(5146..5796)
                     /gene="ulaD"
                     /locus_tag="ACHKA1_001135"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO5678821.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5810..6274)
                     /gene="ulaC"
                     /locus_tag="ACHKA1_001136"
     CDS             complement(5810..6274)
                     /gene="ulaC"
                     /locus_tag="ACHKA1_001136"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO5678822.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6284..6589)
                     /gene="ulaB"
                     /locus_tag="ACHKA1_001137"
     CDS             complement(6284..6589)
                     /gene="ulaB"
                     /locus_tag="ACHKA1_001137"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO5678823.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQYLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(6605..8002)
                     /gene="ulaA"
                     /locus_tag="ACHKA1_001138"
     CDS             complement(6605..8002)
                     /gene="ulaA"
                     /locus_tag="ACHKA1_001138"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO5678824.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            8357..9421
                     /gene="ulaG"
                     /locus_tag="ACHKA1_001139"
     CDS             8357..9421
                     /gene="ulaG"
                     /locus_tag="ACHKA1_001139"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO5678825.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9529..10284
                     /gene="ulaR"
                     /locus_tag="ACHKA1_001140"
     CDS             9529..10284
                     /gene="ulaR"
                     /locus_tag="ACHKA1_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO5678826.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10281..11030)
                     /gene="yjfP"
                     /locus_tag="ACHKA1_001141"
     CDS             complement(10281..11030)
                     /gene="yjfP"
                     /locus_tag="ACHKA1_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5678827.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            11212..11541
                     /gene="bsmA"
                     /locus_tag="ACHKA1_001142"
     CDS             11212..11541
                     /gene="bsmA"
                     /locus_tag="ACHKA1_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO5678828.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11690..11965
                     /gene="yjfN"
                     /locus_tag="ACHKA1_001143"
     CDS             11690..11965
                     /gene="yjfN"
                     /locus_tag="ACHKA1_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO5678829.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(12082..13707)
                     /gene="aidB"
                     /locus_tag="ACHKA1_001144"
     CDS             complement(12082..13707)
                     /gene="aidB"
                     /locus_tag="ACHKA1_001144"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO5678830.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13791..14954)
                     /gene="yjfC"
                     /locus_tag="ACHKA1_001145"
     CDS             complement(13791..14954)
                     /gene="yjfC"
                     /locus_tag="ACHKA1_001145"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO5678831.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(14957..15595)
                     /gene="yjfM"
                     /locus_tag="ACHKA1_001146"
     CDS             complement(14957..15595)
                     /gene="yjfM"
                     /locus_tag="ACHKA1_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO5678832.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15605..16003)
                     /gene="yjfL"
                     /locus_tag="ACHKA1_001147"
     CDS             complement(15605..16003)
                     /gene="yjfL"
                     /locus_tag="ACHKA1_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO5678833.1"
                     /translation="MHILDSLLAFSAYFFIGVVMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(16021..16680)
                     /gene="yjfK"
                     /locus_tag="ACHKA1_001148"
     CDS             complement(16021..16680)
                     /gene="yjfK"
                     /locus_tag="ACHKA1_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO5678834.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16731..17429)
                     /gene="yjfJ"
                     /locus_tag="ACHKA1_001149"
     CDS             complement(16731..17429)
                     /gene="yjfJ"
                     /locus_tag="ACHKA1_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO5678835.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(17448..17849)
                     /gene="yjfI"
                     /locus_tag="ACHKA1_001150"
     CDS             complement(17448..17849)
                     /gene="yjfI"
                     /locus_tag="ACHKA1_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO5678836.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSDNALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEIASLVDNALDLAEITEEYSH"
     gene            complement(17976..18707)
                     /gene="rlmB"
                     /locus_tag="ACHKA1_001151"
     CDS             complement(17976..18707)
                     /gene="rlmB"
                     /locus_tag="ACHKA1_001151"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO5678837.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(18888..21329)
                     /gene="rnr"
                     /locus_tag="ACHKA1_001152"
     CDS             complement(18888..21329)
                     /gene="rnr"
                     /locus_tag="ACHKA1_001152"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO5678838.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(21368..21793)
                     /gene="nsrR"
                     /locus_tag="ACHKA1_001153"
     CDS             complement(21368..21793)
                     /gene="nsrR"
                     /locus_tag="ACHKA1_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO5678839.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(21998..23296)
                     /gene="purA"
                     /locus_tag="ACHKA1_001154"
     CDS             complement(21998..23296)
                     /gene="purA"
                     /locus_tag="ACHKA1_001154"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO5678840.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKERSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(23400..23597)
                     /gene="yjeT"
                     /locus_tag="ACHKA1_001155"
     CDS             complement(23400..23597)
                     /gene="yjeT"
                     /locus_tag="ACHKA1_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO5678841.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(23679..24683)
                     /gene="hflC"
                     /locus_tag="ACHKA1_001156"
     CDS             complement(23679..24683)
                     /gene="hflC"
                     /locus_tag="ACHKA1_001156"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO5678842.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(24686..25945)
                     /gene="hflK"
                     /locus_tag="ACHKA1_001157"
     CDS             complement(24686..25945)
                     /gene="hflK"
                     /locus_tag="ACHKA1_001157"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO5678843.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(26031..27311)
                     /gene="hflX"
                     /locus_tag="ACHKA1_001158"
     CDS             complement(26031..27311)
                     /gene="hflX"
                     /locus_tag="ACHKA1_001158"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO5678844.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(27388..27696)
                     /gene="hfq"
                     /locus_tag="ACHKA1_001159"
     CDS             complement(27388..27696)
                     /gene="hfq"
                     /locus_tag="ACHKA1_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO5678845.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(27782..28732)
                     /gene="miaA"
                     /locus_tag="ACHKA1_001160"
     CDS             complement(27782..28732)
                     /gene="miaA"
                     /locus_tag="ACHKA1_001160"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO5678846.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(28725..30572)
                     /gene="mutL"
                     /locus_tag="ACHKA1_001161"
     CDS             complement(28725..30572)
                     /gene="mutL"
                     /locus_tag="ACHKA1_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO5678847.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPTPRAIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPHEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(30582..31919)
                     /gene="amiB"
                     /locus_tag="ACHKA1_001162"
     CDS             complement(30582..31919)
                     /gene="amiB"
                     /locus_tag="ACHKA1_001162"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO5678848.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPTVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(31938..32399)
                     /gene="tsaE"
                     /locus_tag="ACHKA1_001163"
     CDS             complement(31938..32399)
                     /gene="tsaE"
                     /locus_tag="ACHKA1_001163"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO5678849.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(32371..33918)
                     /gene="nnr"
                     /locus_tag="ACHKA1_001164"
     CDS             complement(32371..33918)
                     /gene="nnr"
                     /locus_tag="ACHKA1_001164"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO5678850.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQDSDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIASLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTEKNHD
                     ESSNSAP"
     gene            33917..35056
                     /gene="queG"
                     /locus_tag="ACHKA1_001165"
     CDS             33917..35056
                     /gene="queG"
                     /locus_tag="ACHKA1_001165"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO5678851.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(35039..35092)
                     /locus_tag="ACHKA1_001166"
     CDS             complement(35039..35092)
                     /locus_tag="ACHKA1_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678852.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(35326..35401)
                     /locus_tag="ACHKA1_001167"
     tRNA            complement(35326..35401)
                     /locus_tag="ACHKA1_001167"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35366..35368),aa:Gly,seq:gcc)
     gene            complement(35612..36157)
                     /gene="orn"
                     /locus_tag="ACHKA1_001168"
     CDS             complement(35612..36157)
                     /gene="orn"
                     /locus_tag="ACHKA1_001168"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO5678853.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            36252..37304
                     /gene="rsgA"
                     /locus_tag="ACHKA1_001169"
     CDS             36252..37304
                     /gene="rsgA"
                     /locus_tag="ACHKA1_001169"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO5678854.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            37401..38369
                     /gene="asd"
                     /locus_tag="ACHKA1_001170"
     CDS             37401..38369
                     /gene="asd"
                     /locus_tag="ACHKA1_001170"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO5678855.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            38391..41714
                     /gene="mscM"
                     /locus_tag="ACHKA1_001171"
     CDS             38391..41714
                     /gene="mscM"
                     /locus_tag="ACHKA1_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO5678856.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VIEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAGLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(41865..43367)
                     /gene="yjeM"
                     /locus_tag="ACHKA1_001172"
     CDS             complement(41865..43367)
                     /gene="yjeM"
                     /locus_tag="ACHKA1_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO5678857.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(43586..44563)
                     /gene="epmA"
                     /locus_tag="ACHKA1_001173"
     CDS             complement(43586..44563)
                     /gene="epmA"
                     /locus_tag="ACHKA1_001173"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO5678858.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            44888..46696
                     /gene="frdA"
                     /locus_tag="ACHKA1_001174"
     CDS             44888..46696
                     /gene="frdA"
                     /locus_tag="ACHKA1_001174"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO5678859.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            46689..47423
                     /gene="frdB"
                     /locus_tag="ACHKA1_001175"
     CDS             46689..47423
                     /gene="frdB"
                     /locus_tag="ACHKA1_001175"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO5678860.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            47434..47829
                     /gene="frdC"
                     /locus_tag="ACHKA1_001176"
     CDS             47434..47829
                     /gene="frdC"
                     /locus_tag="ACHKA1_001176"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO5678861.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            47840..48199
                     /gene="frdD"
                     /locus_tag="ACHKA1_001177"
     CDS             47840..48199
                     /gene="frdD"
                     /locus_tag="ACHKA1_001177"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO5678862.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            48262..49395
                     /gene="ampC"
                     /locus_tag="ACHKA1_001178"
     CDS             48262..49395
                     /gene="ampC"
                     /locus_tag="ACHKA1_001178"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO5678863.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            49484..50017
                     /gene="blc"
                     /locus_tag="ACHKA1_001179"
     CDS             49484..50017
                     /gene="blc"
                     /locus_tag="ACHKA1_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO5678864.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(50014..50331)
                     /gene="sugE"
                     /locus_tag="ACHKA1_001180"
     CDS             complement(50014..50331)
                     /gene="sugE"
                     /locus_tag="ACHKA1_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO5678865.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(50507..50653)
                     /gene="ecnB"
                     /locus_tag="ACHKA1_001181"
     CDS             complement(50507..50653)
                     /gene="ecnB"
                     /locus_tag="ACHKA1_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO5678866.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(50764..50889)
                     /gene="ecnA"
                     /locus_tag="ACHKA1_001182"
     CDS             complement(50764..50889)
                     /gene="ecnA"
                     /locus_tag="ACHKA1_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO5678867.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(50941..51507)
                     /gene="efp"
                     /locus_tag="ACHKA1_001183"
     CDS             complement(50941..51507)
                     /gene="efp"
                     /locus_tag="ACHKA1_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO5678868.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            51549..52577
                     /gene="epmB"
                     /locus_tag="ACHKA1_001184"
     CDS             51549..52577
                     /gene="epmB"
                     /locus_tag="ACHKA1_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO5678869.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVITSQDEFAIAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            52972..53841
                     /gene="yjeJ"
                     /locus_tag="ACHKA1_001185"
     CDS             52972..53841
                     /gene="yjeJ"
                     /locus_tag="ACHKA1_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO5678870.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(54034..54387)
                     /gene="yjeI"
                     /locus_tag="ACHKA1_001186"
     CDS             complement(54034..54387)
                     /gene="yjeI"
                     /locus_tag="ACHKA1_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO5678871.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(54525..56171)
                     /gene="groL"
                     /locus_tag="ACHKA1_001187"
     CDS             complement(54525..56171)
                     /gene="groL"
                     /locus_tag="ACHKA1_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO5678872.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(56215..56508)
                     /gene="groS"
                     /locus_tag="ACHKA1_001188"
     CDS             complement(56215..56508)
                     /gene="groS"
                     /locus_tag="ACHKA1_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO5678873.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            56784..58040
                     /gene="yjeH"
                     /locus_tag="ACHKA1_001189"
     CDS             56784..58040
                     /gene="yjeH"
                     /locus_tag="ACHKA1_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO5678874.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(58056..58532)
                     /gene="fxsA"
                     /locus_tag="ACHKA1_001190"
     CDS             complement(58056..58532)
                     /gene="fxsA"
                     /locus_tag="ACHKA1_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO5678875.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            58869..60305
                     /gene="aspA"
                     /locus_tag="ACHKA1_001191"
     CDS             58869..60305
                     /gene="aspA"
                     /locus_tag="ACHKA1_001191"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO5678876.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            60423..61724
                     /gene="dcuA"
                     /locus_tag="ACHKA1_001192"
     CDS             60423..61724
                     /gene="dcuA"
                     /locus_tag="ACHKA1_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO5678877.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            61840..62178
                     /gene="cutA"
                     /locus_tag="ACHKA1_001193"
     CDS             61840..62178
                     /gene="cutA"
                     /locus_tag="ACHKA1_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO5678878.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            62154..63851
                     /gene="dsbD"
                     /locus_tag="ACHKA1_001194"
     CDS             62154..63851
                     /gene="dsbD"
                     /locus_tag="ACHKA1_001194"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO5678879.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            63888..64463
                     /gene="yjdC"
                     /locus_tag="ACHKA1_001195"
     CDS             63888..64463
                     /gene="yjdC"
                     /locus_tag="ACHKA1_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5678880.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            64570..64645
                     /locus_tag="ACHKA1_001196"
     tRNA            64570..64645
                     /locus_tag="ACHKA1_001196"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64603..64605,aa:Phe,seq:gaa)
     gene            complement(64841..65878)
                     /locus_tag="ACHKA1_001197"
     CDS             complement(64841..65878)
                     /locus_tag="ACHKA1_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5678881.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRRYVLKEQKRREVTWNNKVAHLRAIMGFGIKMKLLPQTENPL
                     VEASVASTEKKKKTLSDNQMLRVYLVVQRFAEAEEQNMVNSRRRNAIYPAWYWLTVLD
                     VLRYTGMRFNQLKHIRVRDVCPDESIIDLKLEGSKTHREWGVPLVAPLKVSMEKLLER
                     ARQCGAGDDDYLFDVCRFSEPISADRYQYCPVRAHQTVRSFFRRLSRECGFPVSPHRF
                     RHTLATRLMKYPERNLHLVKSILGHRSLTTTMGYVDVCMESAAQTLEDALSLYTDERT
                     VSKICKGGNKS"
     gene            complement(65946..67427)
                     /locus_tag="ACHKA1_001198"
     CDS             complement(65946..67427)
                     /locus_tag="ACHKA1_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFO5678882.1"
                     /translation="MLERLKRLFPVKTECSDVRTDIPGETVPEGYFTPLPADVLVSTP
                     FRKRCLKQLWENSSMPVDMYQRLCLDPVHQLLSVSQNLPATAEGRWGYTGGFGDLTLQ
                     FTTYAVRLARGRMFPPGATAEEQAAQGSVWNMVVFWSALFYHLPLLAELEGEQADGRC
                     WQPGISVPEGVYRFRFRKMPCQGTEGRQLAALMAGQLLPGEAGAWLATVPGALQNLAG
                     AVWHQHPEMALIRTVLQEAMEEVVSPLAAGDVAEVPVAEVVPVLLQSAAISAESVIQP
                     LPVVQSPVEDAENEGRTAEQEVDEGNETDADTGMLLTLFSAADLSDEGGQIASGVPPD
                     KESGIELPEESGCVTENQGNRIPETVEKTAEFSLTEDSRVESEFIRWLKEGLREGSLS
                     VNSVDSNVHVVSGYVFLCAPGIFYRFLKESGSGQDRRYVQSLFERAGIHKVRSGERFF
                     QARLYSDAEHSGRYKMVSGYLVKGRFLYGNITVPGDSPLIVFG"
     gene            complement(67420..68910)
                     /locus_tag="ACHKA1_001199"
     CDS             complement(67420..68910)
                     /locus_tag="ACHKA1_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFO5678883.1"
                     /translation="MSFNPTDEQYAIINWQGQQLVVNAFSGTGKTSTLVQFARASPDS
                     RMLYLAYNRAIRDEAERKFPFNVECRTSHQLAYARVGRHYRHRLVPGLRITDVARKLN
                     TRYWALARVAGTGLNHFICSADAVPGLHHLPDKDEMRGVPPADALRAVQLLWNEMSNP
                     GGTFPVTHDTYLKLFQLSGADLSHRWDTILFDEAQDANPVTTALVLGQKCRVVLVGDC
                     YQQIYRFRGANNALSHPALKNADRLWLTQSFRFGPAVARMANLLLQREGETREVKGCG
                     GDDEVLLKYHAREHLQGHYTVLSRTVAGVIGTALMAAMKGQKVYWVGGIEGYRTGELE
                     DLYWFQVDMPERMHSDRLRRDYRNFEEYKYIAKSTKDVEMNQSLRLLELCFPLPKKLE
                     LLRQYTVTNEQDADITVSTAHRSKGLEWPVVMLDEDFQDITDPLMTEAERHDETCLLY
                     VAVTRALRTLVLNDLMHQLAEEQQKTEGLRGTGYDTSGRVQENGHA"
     gene            69142..>69840
                     /locus_tag="ACHKA1_001200"
     CDS             69142..>69840
                     /locus_tag="ACHKA1_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678884.1"
                     /translation="MSEVKKHVSYSLTVAAMLSAAIVCQTTHADINTPNITGEKNNTT
                     TRFSGNIYGSENTVTSESNSLVVGNSNSVEGGYNNIVIGNSSTVKASGLIRKVGEDIV
                     LDGYSVSLGNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIVSVGSDTLKRR
                     ITNLAQGKDDHDAVNLMQMTSAVNREAGERIKSTNNLQNQLNTLSGQVITETRARTEG
                     DATALAAARAHSDEN"
BASE COUNT        17024 a        18898 c        17176 g        16742 t
ORIGIN      
        1 caggccaagc gtatgctggt tgatcaaata atatccccca agcgcagcaa tattaatatt
       61 cacctgaaat gggtcgatat ctggcttaaa aatattgtgt ttcttccctt catccaataa
      121 ggattccatt aactgcaagt gcgcatgggt aatttctaag agacgctgtg attttgcata
      181 atgcacacct ttactttgat tttcactatg cacaattttt aaaaaccagg gattagcaat
      241 ataataatcc catgtaaaat caataatttt ctcaatggct ttttgtggct ggctaacctg
      301 ctgattaacg aatacaccga taccgacagc gtagaataat tacataccgc acaacaagaa
      361 tgtacggtgt aaggcgtgga agtcattttc tccccgggtg catcgttata tccataatga
      421 gcgatttttg atattaatat ctttcaccat ccgctatttt caccacttca gaaaacaaac
      481 ttatttttat taatatcaat tctttttctt tgaacattca actgcgatat ttgtaaaaat
      541 attaaacagg caacaataaa atgacattac caacaactat ttattcattc cccgcatatt
      601 tacctcgttt ttcgtccacc gacaaaccag caaaactaaa atttcatcaa tacgcacgcg
      661 ctacattact gtcaaatcgt ggaagagatc ataattgcga tggcagacgt actgttgaga
      721 tccacaaact agatctctct gactggcaag cattcaataa gttagcaaag aagtgtaatg
      781 cttacgatgg agtaacaatg aatggtgata attcttttgg atggaaccat gaaactacat
      841 tagataatat tcacgcccag aaatacaata aagcttacgc gggtgcgagg ttaaccgcag
      901 aactgaaata tttattgcaa gatgtcgaat ccttcgaacc caacagtaaa tacacaatac
      961 acgaagtggt tttgggacct ggttatggta ctccagacta taccggccaa acgataggat
     1021 acgttgtaac ccttcctgcc caaatgccta attgctggtc atctgaacta ccaacaatcg
     1081 atctttatat cgaccaactt cgcactgtta caggcgtttc taatgccctc ggattcatca
     1141 ttgctgcatt gctaaacgcg tattctgatt taccccatga tttgaaaatt ggtctgcgtt
     1201 ccttatcaag ttcagccgca atctactcgg gtttgggttt cgagcgagtg ccgcaggagc
     1261 gagacatcag ttgcgctcgt atgtatctaa cgccagcaaa ccatcctgac ttatggacac
     1321 aagagaatgg tgaatggatc tacttgcgaa actaatacct gacgaaaaat agaaagcaaa
     1381 acgccgacca atggtcggcg tttttacgtc tcgttgaata acgaattatt cagctactac
     1441 gtttacgatc actttcgcga atacttcgct gtgaacctgg aagctcactt cgtgttcgcc
     1501 agtggtacgc agaacgccgt tcggcagacg aacttcgctc ttagccactt caacgccagc
     1561 tgcagttaca gcgtcagcga tgtcgcgagt accgatggaa ccgaacagtt taccttcgtc
     1621 gccagcttta gacgcgatgg taacagtttc cagtgcattg attttctcag cgcgagcatt
     1681 agctgctgcc agaacttcag ccagtttagc ttccagttca gcgcgacgtg cttcgaagaa
     1741 ttcaatgttt ttcttggtag ctggaacagc tttaccctgc ggtaccagga agttacgagc
     1801 atagcccgct ttaacgttta cctgatcacc caggctaccc aggtttgcta ctttatcaag
     1861 cagaataact tgcattacct tatcctctca aagtcgtatt aatggaccgt gaccgattac
     1921 tgatggcgat cagtgtacgg cagcagggac aggtagcgag cgcgtttgat agcgcgagcc
     1981 agctgacgct ggtattttgc acgggtaccg gtgatacggc ttgggacaat cttaccgctt
     2041 tcggtgatgt agtttttcag cgtagcgata tctttatagt cgatctcttg aacgccttcc
     2101 gcggtgaaac ggcagaactt gcgacgacgg aaataacgtg ccatatggct agtctccaga
     2161 atctatcaat tcaatctgct cggcatgcaa aaccattttg ctcagtccgt tctttgcctt
     2221 gtggcatgaa atgaacccct gaacggttat gcgactgccg accgttatac tgtgagtaat
     2281 ggcctggttt tcgtgtccgc taacaataac gggcatttga caccatgcct gccggtgaaa
     2341 gccggcttcc tcctgcacag aacgatgctc aagcacgaac tggcagtgag gaattcctga
     2401 tggactgacc tttcgaaggg gagtcctgca cacggtgccg gacaacacca gacggttggt
     2461 catcagaaat tactcttcag aatccccagc ttcagcatca tcagcggttt cgtttgcgaa
     2521 atcatcgcga cgctcacggc gctcgtcttt cgctttaacc atcggagatg cttcggtaac
     2581 agcgtgcttg gtacgcataa ccatgctgcg gataacggca tcgttgaagc ggaaggtagt
     2641 ttccagctca tcgatcactt cctgcggagc ttcaacgttc atcagaacgt agtgagcttt
     2701 gtgcagtttg ttgatcgggt aagccagctg acggcggccc cagtcttcca gacggtggat
     2761 cttgccttct gcaccagtga tggcagcagt gtagcgctcg atcatgcccg gaacctgttc
     2821 gctctggtca ggatggacca taaaaacgat ttcgtaatga cgcatcgaat tgctccttac
     2881 ggattattca gcctcctgtc tgggtcagcc gaatcccggg gaggcaagga acgtgttaaa
     2941 ggtcggctga aaaatgacgc gttatagtac tgacataccc cgctaaactc aaggccagcc
     3001 gacaaataaa tctcatcttt caccaaagtg gactaacacg ctgatttgaa gttcatcaca
     3061 cttcatcatg gtgttttttt gaacaactgc atcagaaatg gtcaccatca acttctttat
     3121 cattgattaa aattaaatca gaagatcatc acagaggaga gcacacaaag tcagccgtcg
     3181 aggaggtatc tatgttcagt cgtgttttag cccttctggc tgtgcttttg ctaagtgcaa
     3241 atacatgggc agccattgaa attaataacc accaggccag aaatatggac gatgtgcaaa
     3301 gcttaggcgt gatttatatc aatcataatt tcgccactga aagcgaagca cgtcaggcat
     3361 taaatgaaga aacagatgcg caaggcgcaa cgtactacca cgtaattctg atgcgggaac
     3421 cggggagtaa cggcaatatg cacgccagcg cggatattta tcgctagcac caggtataac
     3481 caacgaaaca ttgccatagt ttgctttgcc cccttcgcag ggggcttttt ttgaaaaacg
     3541 gctgcggaat tagaccagtt atctcccgag gaaggaaatt tccgcagcgc gtgttctact
     3601 tctgcccgta ataagcgtta ggaccgtgtt tacgcatgaa gtgtttattc atcaggaagc
     3661 tgtcgatgtg attcagttgt gggttaatgc cgcgcgcaat ccacgccatt ttcgccactt
     3721 cttccatcac caccgcgtta tgcaccgcat cgtgggcatc tttcccccag gcgaacggtc
     3781 cgtgctgata caccacaatt cccggcgtat gcagcggctc ggcgttgccc agcgtttcga
     3841 taatcacttt gccggtgttc agttcatact cgccctgcac ctcttcttcg cttaacccgc
     3901 gcgtacacgg aatgtcgcca aagaagtagt cagcgtgcgt ggtgcctaac gccgggatcg
     3961 ccagccccgc ctgcgcccat gcggtggcat gagtggagtg ggtatggaca atgccgccaa
     4021 gcgacgggta acgacggtag agttcgagat gcgtcgcagt gtcggaagat gggcgatact
     4081 ccccttccac caccttgccg ctcatatcaa ccaccaccat atcggccgct ttcatggttt
     4141 cgtaggcaac gccgctgggc ttgatcacca ccagcccgcg ttcgcggtcg atagcgctga
     4201 cgttgcccca ggtaaaggtc accagcccgt agcgcggcag ctccatgttg gcttcaaata
     4261 cctgctgttt tagcttttgc attatgccgc ctccaccatg cccgctttcg ccatgcgcgc
     4321 tttcacccaa tcacgcgctt tcgccacttc tgccgccggg tcttccgccg tttcgctcca
     4381 catctcaatc aggtacggcc cgcaatagcc actctgtttg agcgtttcga aacaacgttc
     4441 gaaatccact acaccttcgc caaacggcac gtttttgaag acgcccggtt tggtgtcttt
     4501 cacatgcacc gcgacgatat gcccgattcc ggcctgcaac tccatctgca cgtcgttatc
     4561 ccacgccgac aggttgccga tatccgggta gagctggaac cacggattgt tgagatagtg
     4621 cgcgtagccc agcgccttgc tgatggagtt catcaacgga taatccatga tctccatcgc
     4681 cagcgtcacc tgcgcgcggc ttgccatctc aacgctctct ttcaggccgt cacggaaacg
     4741 acgacgcgtt tcgttattgg cttcctgata gtaaacgtca tagcccgcca gctggatcac
     4801 gcgaataccg acatcctggg cgaactggat agctttacgc ataatctcca gcccctgcgc
     4861 ccgcaccgcg tcatcttcac tgcccagcgg gaaacgacga tgagcagaaa ggcacatgga
     4921 cggcacgcgc acgccggttt caacaatcgc attgaccagc gccagacgct gctcgcggct
     4981 ccagtcgagg cgcgacaggc gctcgtccgt ttcatctacc gacatctcga caaaatcgaa
     5041 gcccaacgtt tttgccagtt gcaggcgttc cagccagcac tccccggcgg ggagcgcttt
     5101 ttcatagatg ccaagcggga tttgtttgga caacatatcc gctccttagc cccacagttc
     5161 agcgatggaa cgtttgaact gacgtgcggc ttccaccgga gaagcggcat cacggatact
     5221 acggcccgcg ataaagacgt gaatcggaat acccttgaac agcggcagat cttccagcgc
     5281 caggcctccg gtgacggtga ctttgaagcc catatcggaa agacgtttga tcgcggtgat
     5341 gtccgcttcg ccccacgcca cgcctgcggc ctgcgcgtca cggctgcggt gataaaccac
     5401 ctgctgaatg cctgcatcgc gccactgttg cgcctgttcc caggtccagt aaccggtcag
     5461 ttcgatctgc acgtcgccgt taaactcttt tgccacgtcc agcgcgcctt tggcggtgtt
     5521 gatatccgca cagcaaatta ccgtcaccca gtcagcgttg gcttcgaagc acatacgcga
     5581 aaggattttg cccgcatcgg caattttggc gtctgccagt acgattttgt gcgggtaaag
     5641 cgctttcaga tcgcgaaccg cacgcacgcc ttcgcccacg cacagaatgg tacccacttc
     5701 gataatgtcg acttcttcgg caatcaggcg agtggtttcg taggcgctat ccatagtctg
     5761 gttgtccagc gcgacttgca acatcggtaa tgacatgttc aattccttct taagctgccg
     5821 cgttggtgcg gtcaatgaga tccagtactt cctgctcggt acggcaggcg cgtaaacggt
     5881 caaaattctc ttcatcttca aacaggttga cgatctgcat gatgcccact tcctggtgag
     5941 tattggcatc gaccgccgcc atggtgatga ggatatccac cggatcgttg tcgtcgtgat
     6001 taaattccag cggctttttc agcgttacca gagagaaacc tgttttctta acaccctctt
     6061 ccggccgccc gtgcggcatc gccagccccg gagcgataac gaaataaggg ccaaactgtt
     6121 ccacgccatc cagaatcgcc tggtagtaac gcggctcgac aacatccgcc gccaccagca
     6181 ggtcaacgcc gatttttact gcgtcctgcc atgtctctgc ttcagcctgc aggcggatag
     6241 atttattctc cgccagcgaa tcacgtagtt tcatggcgta tccttacttc acgtcctgcg
     6301 ggaaatgctc tttgatcact tccagcagtt tcgggccaaa gtcggcagga gagagcatgt
     6361 tgcgcacgcc aaccacatat ttgttgccgg taacggtgat ttcgcccgcg atgtgcgtag
     6421 aagcgatgat gatatccgcg ccgctcaact cgcttttgta ctcgccaacc gcgcagctgt
     6481 ttaccgtgtg gtcaatgttt gattgggtta aatactggtc cactttcatt ttcatgatca
     6541 tggaactgcc ttgtccgttg ccacacacag ccagaatacg tacggtcata atcaaaactc
     6601 cttattaagc agactgttct gccagttgtt tttctgcatc ttcttctgcg cgcagtgcgc
     6661 ggccagcgaa gaacatataa gccagtgcaa ttacaatgat gacggccata aaggcgatac
     6721 cgatggagaa gaagccttgc atcatcggcg gtgccagaat cgaccagtcc gccatgccca
     6781 tccaggcact cataccggtg agtttcaccg cccagacgca accaaagatt tcgatcattc
     6841 ccatcaccag acaaatcttc agcgctgcac gccagccgcc gaagtggtta gcgaacacgc
     6901 cgatggtggc gttggagaag aacatcggga taaagcctgg aataatcaga atggaagagc
     6961 cgcaggcgac caggatgcca actgcaatca gctgaccgat ggtgccccac ataaagcccc
     7021 agaccacggc gttcggcgcg aagctataga tagccgcaca gtcaatcgcc agaaccgcac
     7081 ctgggatcag gcgctgggag ataccgttaa atgcttcaga gagttccgcc acaaacatgc
     7141 gcacgccctg agtgatgatg aagatcgcca ccgcgaagga gaaaccggtt tgcaggatgt
     7201 agaccgtcca gtgcactttg cccgccatcg cctgcacggt gtcgataccg aaggagagca
     7261 gaatggcgcc aaagaagatg gtcatcacaa tcgccgtgga gacgatgttg tcgtggaaaa
     7321 tgttcagcca gcccggcaat ttgaggtctt caacgctctc ctcttttttg ccgaggaacg
     7381 gtgcgacttt ataggcaatc catgatgcaa actgctgctg gtgaccgatg gagaaaccac
     7441 agccatccgt cacttcctga gtcggcttgt acatcatgtt ggaagtgatg ccccagtaga
     7501 gcgaaaccag aatcgcggta cagataatgg tggtccacat ggagtagccg aagataaaca
     7561 gcgtaacggc aatcaacccg gcctgctgga acatgatgtg gccggtcaac atgattgtgc
     7621 gaatgccggt aatgcgacgc agcagcacgt aacagatgtt cagcgccagc gctaacaata
     7681 cggcgtaacc cacccagcta taggcatcgc ccatgcggtc gatggttgcc atcatcgaag
     7741 cgtaagtatc tgaaattgcg ccgttaatgc cgtagacttc ggacattttc gccaccaccg
     7801 gtttgaaggt gctggtgagg atgccggacc ctgcctgcaa caacatgaaa ccaattatgg
     7861 ttttaatcgt gcctttaata ataacgctga cacttttgcg cagtaggatg tagcccagac
     7921 aggtcacaat acccagcaac aacggggcat tggtcatgac ctggttaaaa aacacggtaa
     7981 agatgttgta gaggatctcc ataacgatct cctgaagaag aggtaaccgg gtattcctca
     8041 cacccggaat gttgtgcact cactctaatt ttcaaaagta atcacaacaa gattatttat
     8101 gattaaatgt gacgcgacac acaaataatt ccataacgaa aatatgggat tcacacaaag
     8161 aaacaattaa tcatttaaat tcattaagtt aattgaatta cacattccat taaatctttc
     8221 catactccaa attcacaaca aatcacccgg caaaaagtca aaaaatggtc attgccttcc
     8281 tgaaaaaata gtgtcaggat taatcaaaac caatcatatg ttgattcgaa ttgaactatc
     8341 atgaaggtaa atggcgatga gtaaagtgaa aagtatcacc cgtgaatcct ggatcctgag
     8401 cactttcccg gagtggggta gctggttgaa tgaagaaatt gaacaagagc aggtagctcc
     8461 tggtacattt gcgatgtggt ggcttggctg caccgggatc tggctgaaat cggaaggtgg
     8521 caccaacgtt tgcgttgatt tctggtgcgg cacaggcaaa caaagtcacg gtaacccgtt
     8581 aatgaaacag ggtcaccaga tgcagcgtat ggctggcgtg aaaaaactgc agccgaacct
     8641 gcgcaccacc ccgtttgttc ttgatccatt tgctattcgc cagatcgacg ccgtcctggc
     8701 gactcacgat cacaacgatc atatcgacgt taacgtcgct gctgccgtga tgcagaactg
     8761 tgctgatgac gtaccgttta tcggaccgaa aacctgtgtg gatttatgga tcggctgggg
     8821 cgtgccgaaa gaacgttgca tcgtggttaa accgggcgac gtggtgaaag tcaaagacat
     8881 tgaaattcat gcgcttgatg ctttcgaccg tactgcactg atcaccctgc ctgccgatca
     8941 aaaagcggct ggcgtactgc cagatggcat ggacgatcgc gcggtgaact acctgttcaa
     9001 aacgcctggc ggctccctgt atcacagcgg cgactcccac tactctaact attatgcgaa
     9061 gcacggtaac gaacatcaga tcgacgtggc gttaggatcg tacggcgaaa acccgcgcgg
     9121 tatcaccgac aaaatgacca gcgccgatat gctgcgtatg ggcgaagcgc tgaatgcgaa
     9181 agtagtgatc ccgttccacc acgatatctg gtcaaacttc caggccgatc cgcaagagat
     9241 ccgcgtgctg tgggagatga aaaaagatcg cctgaaatat ggctttaagc cgtttatctg
     9301 gcaggtgggc ggcaaattta cctggccgct ggataaagac aacttcgagt accactatcc
     9361 gcgcggtttc gatgattgct tcactattga accggatctg ccgttcaagt cattcctgta
     9421 atctgtcgtt tataccggat agcgtggaga agttcgcgct atccggcgta gtatttcctg
     9481 gctatttcaa atatcatcta aaaaaatcaa attttatcgg aatagctcat gactgaagca
     9541 caaagacatc aaatcctcct ggaaatgctc gcacaattgg gctttgtgac cgttgagaaa
     9601 gtcgttgagc gtctgggaat ttcgcctgcc actgcgcgac gcgatatcaa taaacttgac
     9661 gaaagcggca aactgaaaaa agtgcgcaat ggcgcagaag ctattaccca acagcgcccg
     9721 cgctggacgc cgatgaatct gcatcaggcg cagaatcacg atgaaaaagt acgtattgct
     9781 aaagcggcct cgcagctggt taatccgggc gaaagcgtag tcatcaactg cggctccacc
     9841 gcgtttctgc ttgggcggga aatgtgtggc aagccagtgc aaatcatcac taattatcta
     9901 ccgctggcaa attacctgat cgatcaagaa catgacagcg tgatcattat gggcggacag
     9961 tacaacaaaa gtcagtccat cactttaagc ccgcagggca gcgaaaacag tctctatgcc
    10021 gggcactgga tgtttaccag cggaaaaggg ctgaccgcag aagggttgta taaaaccgat
    10081 atgctgacag caatggcaga gcagaagatg ctgagcgtgg tagggaaact ggtggtactg
    10141 gttgatagca gtaagattgg cgaacgcgcg ggaatgcttt ttagccgtgc cgatcaaatc
    10201 gatatgctta tcaccggcaa aaatgctaac ccggaaatcc tgcaacaact ggaagcgcaa
    10261 ggggtcagca ttctgcgtgt ttaaagatgc tggcggaaaa atgtcacggc agcatctaac
    10321 gcctcaggcg taatgcggtg acgcacgcct ggctgccatg aacaggttag cagtttatcc
    10381 cgtcccgtct cgcttaaggc ctgctgcaaa cgtagtgatt catcggcagg cacaacgtca
    10441 tcgtcgaggc catgccacag cagtagaggt ctgtccccaa gttgttccag gtggtttgtc
    10501 gcttcccact ctgccagcgg cgcgacaatg ttattgaatt cattctgctg tgctgccgtt
    10561 tcaggtatca gcggtggaaa cagtgaacgg gcgagcgatg taaaatagcc cgatcccatc
    10621 atgctggcgg tacatttcac cgtggggtgg cgagcggtaa ttcccagtgc cgtcatcgcg
    10681 cccatcgacg cgccaccgac tgccagacgg tcatcaagca gccagttttc ttcggctatt
    10741 gccgcacgta aagtagtgaa ttcctgcata ctttgtagca agatttgcca gaattgattt
    10801 aaccgccgcg ctgcgtcgcc actaaaacga ctaccgtgat cgggcgcatc cggcatgatc
    10861 acccgcaaac cagcctgcgc cagcgcaacg gcaaaatagc tatacaccag actggacgaa
    10921 gtaaagccgt gataaaaaat cacgcacggt aacggggtat ctttttgtcc gacaggataa
    10981 gcatgaagaa cgggaatatc tgccagctcg cgtgattcta tttcaatcat ctgtctctcc
    11041 tttttgtctg gtatgacatg aagaaatcct tagcacaagt ttctgctggc ggtattagtt
    11101 cataatgttg agatgtgggt tacgctttcg tcacgttttc attcgaaaat cgcgtaccag
    11161 gtaacaattc gggaacattc ccccaaagtt aaattaataa ggcactacac tatggttagc
    11221 aggaaacgta atagcgttat ttaccggttt gccagtttat tattggtgtt gatgttaagt
    11281 gcctgtagcg cactgcaagg tacgccacag ccagcaccgc cagtaacgga ccatccgcaa
    11341 gagattcgcc gcgaccagac gcaagggtta cagcgaatag gtagcgtaag cacaatggtt
    11401 cggggttctc cggatgacgc attagcagaa atcagagcaa aagctgtcgc tgcaaaagct
    11461 gattattacg ttgtcgtcat ggtggacgaa accatcgtga caggacagtg gtattcacaa
    11521 gccattttgt accgtaaata aatcagacaa cttacatagt cagctttaca ttgctttgcg
    11581 tccctatgcg tttacctgtg cacaataaat taccggctgg aagcctgggc gtttattacc
    11641 gcgatggaat gccctgcaac atgtggggaa acgaaaaatg gagctgacga tgaaacaatt
    11701 acttgcctca ccctcgctgc aattagtgac ttatcctgcg agcgccacgg cacagtctgc
    11761 agaattcgcc agtgctgact gcgtgacggg cctgaatgaa ataggtcaga tctcagttag
    11821 caatatttca ggggatccgc aggacgtgga acgtatcgtg gcgttaaagg ccgatgaaca
    11881 aggtgcgtca tggtaccgca ttattaccat gtacgaagac cagcagcctg ataactggcg
    11941 cgtacaggcg atactgtatg catgagtgtc atctacagca aatgaacaat gccggatgcg
    12001 gcgcgagcgc cttgtccggc ctacaatttt cacatatttc attgagttaa tcgaaaccag
    12061 cgtcgcatca gtcgtatacg cttacacaca cactcccccc gtcgcccgca gcaataaatc
    12121 attctggatc tgctctgaca accgtacgcc gccgcgcgta tctaacatca cctgacacca
    12181 cgcctgcgcc attggcggag aggcatattt caacatttgc gcaccgcagc ccagcaggaa
    12241 tagctgatga gtaatctctc gccccagttc ttcagctggc ttacgcagct gctgctgtaa
    12301 acgacgaacc gcgcgatcaa aatagcgatc ctgccctttc acttccacaa atgcttccga
    12361 caataagtcg tatacgcccg cttgcttatt gagaacgcgc agcacatcca ggcacataat
    12421 attgccggaa ccttcccaaa tactgtttac cggcatctcc cggtaaagcc gcggcagctc
    12481 gctctcctcg caataaccaa tgccgcccag cacctccatc gcttcggcca caaacggcat
    12541 acctcgtttg cagatcacaa attttgccgc aggcgtaaat aaacgcgccc acagggcttc
    12601 tttggcatcg gctcgccggt cccacgctcg cgcaagacga aacagcaacg ccgtttgccc
    12661 ttcaagctga agtgccatgc gacttaagac atgacgcata aggggctgtt ggatcaatgg
    12721 attaccaaaa acatggcgtt gatgcgcatg ataaatcgcc agcgagaacg cacggcgcat
    12781 catggcatgg ctacccaggg cgcaatcaaa acgcgtcatc ccccccattt tcaggatcag
    12841 acgaattcct tccccttcct gccccaataa ccagccaatg gcatcctgaa actccacttc
    12901 acaactggcg ttagagcgat tacccagctt atctttcagc cgctcgaggc gaatcgcgtt
    12961 gcgttgcccg tcaggcaaaa agcgcggcac aaaaaagcag gacaaaccgc ccgcggtctg
    13021 cgccagcacc agatgcgcat cgctttgcgg aaccgagaaa aaccatttat gccccaccag
    13081 ccgataagag ccatcctcca gacgctctgc gcgggtggtg ttgctcatga catcggaacc
    13141 gccctgcttt tccgtcattc ccatgccaat caacaaaccg cgtttttgcc cacctggcaa
    13201 taagtgagaa tcgtagcgat cgctcaacag cggcgtggtc cagtcctgaa acggcgtggg
    13261 taacatctgt aacaacaatg gcgtggcggc aaaggtcatc gttattggac ataacgaccc
    13321 cgcctcaacc tgtgcgtgca acataaaacg cgccgcgcgc gccacaaatg cgccggagcg
    13381 agcgtcttct tcccaggcaa gattgtgcac ccgattggta catagcgcct gcatcagcag
    13441 gtgccaggcg gggtgaaaac gcacatcgtc cagacggcgt ccttgcgcat cgtagcgcaa
    13501 taattcaggc ggattcacat tcgccagccg ccccagttca agggattcag ccgttcctaa
    13561 ctgctgacca atactggcta gaaaatcgct atcccagcca gcaccttcac gcgttaccgc
    13621 ttcgcaaagc gcgccatcag acaggtataa gttgctgtta tttaatggta taggttgatt
    13681 aaaaacggtg tgagtttgcc agtgcactgt gtctccctcc atcaatggca gtcaccatta
    13741 gtatggtcac tgccatggat tcatgacaga aagagattgc taaaacattc ttatccagca
    13801 atgtaatgcg gaacgaaacg tgaggtgtct ttggtgatca gtgtgttatc ttcacgaatc
    13861 cccattccgc acgcttcatc atcgacaatc cagctaccga tgagtgtgta gctatcgcca
    13921 aaccgcggca gaggttgaaa cgcctggtag atcatcggtt catcggcgta atcaccatca
    13981 gcgtggtcaa cgacgttatt ctggccgtcg aaaatggtga catttccgcc ttcgcgcgag
    14041 tagatcggtt tacgcacata gctttcgcca gcggcaatct gcggtttttc accctcaaac
    14101 caggatgcaa gaaggttagg atgaccaggg aagaagcgcc aaagcagcgg cattagccct
    14161 ttattactca agatactttt ccataacggc tccacccatt gctcgcgacg cttgcgcagc
    14221 agcggaccgt tatcgtcacg catcatccac tccagcggat acagcttaaa tgcacgctgg
    14281 atgacattat catcaagatc ggtcagtacg ccgccgacgc ccaaaccgag atcttcaatg
    14341 tagataaacc gcgactcctg ccctgcctgc tgggcgcagt cctgcaagta cagcacggta
    14401 gtcctgtctt catcggtgtc ctgacagcag cagaaataaa acggctcccg actgtaaagc
    14461 tcactaaagc gcgaaatcag gcgttcctga atggcattgt actgatcggc atcacgcgga
    14521 ataatgccgc tgcgccgggc atcctctagc cacaaccact ggaaataagc cgactcatac
    14581 aacgaggtcg gcgtatcggc gttgtactcc agcagcttca ccggcgcatt gccacaccag
    14641 gcaaaatcca tccggccata cagcgaagga tcgcgtgcgc gccagctttc agcgatcaca
    14701 tcccagtaca acggagggat cgccagttgc gtcaggatct cttcgtcttt aaccgcgcga
    14761 tccaccacct caaggcacat ctgatgcagt tccgcagtcg gtttttcgat ctgctcttca
    14821 atctggcgca gagtaaaacg gtaagcccga ctctcgtccc aatagatttc attgtcgatg
    14881 atatgaaagt cgaaaccgtt gtcagcggcg atttggtcca gatcccgtcg cacaggaacg
    14941 ttgtgtctca gcatgattag cctccccaat gcccacgggc gctggaagaa cgaccatagc
    15001 cgccgcgaga aacggttgac gcttttttgg tggtaaagcc gcctgaagag taagactctt
    15061 ttttgccaga accggagcgc caggagtaat caccagaagt gttgcgccag acggggcggg
    15121 aagcaaatga ggatccgccg ctgttataaa caaactgctc atcgcgatct ttacgaataa
    15181 cccggcttaa caaaaatcca gaaacgaccg ggatccaact ctgttcaacg ttgtcgtagt
    15241 agcaattttc gtacttagac tgacaattct gctgagtcat attcttcgga acatcggcat
    15301 aaaatgctgc tttggcgtta ttccagccac gcgcgcaaat gtctgcatta ttaccgtcat
    15361 caatacaatc ctgcactgtc gcgtaaaacg ttccgtcgcc gtcgttatcg acatcgccac
    15421 tatcgccaca acctttcaat acaaaaaaag cggcaccgcc cattagcgcc aacgttaagt
    15481 atttttgtgc gtagcgattg cgacgcggtt caaaaggatt attcggtcta ccaatgcgac
    15541 tgatcgctcc gtgaccaatt ttactgttat ttcttgattt gcgttttctg gccatgatcg
    15601 tccattacca tgtcatacaa gctgcgttaa aaataccgcc agccagcgcg gcggttccca
    15661 taaacattcc tgctgcggta ttgtgattaa taattttttc gcttaatgcg ggcatataaa
    15721 gtctgacacc agcaaaaacg agtaactgaa tcaccagtgc aatcccgccc caggcgaaat
    15781 agtctggaat actcaccgca ttgattgccg cactggataa ggggatcacg taacccaaca
    15841 atgtaccgct gaacgccagt gacgctgcgg tattattgtt tttgattaat tgccattcat
    15901 tgtgcggtgt aattttggag tagataaaca gaaaaataat caccatcacc acgccaataa
    15961 aaaaataggc actaaaggca agaagtgaat ccagtatatg cattgtaaac gtccttgtaa
    16021 ttaaccaata atatgaagtg atgtcagcgg gatatcgaca cccaacgcgc gcgaaaataa
    16081 ccactctggc tcgccgagat cgttaaaaga ttcttcacca tttagcagca aatattcgta
    16141 agtatcttcg gtcacttgcc gctggtagcc catggtaaaa ttatggactt cccatttagc
    16201 atgattttga ttttctactt tttccagcat gtagacgggt tcgatatttc ccggttcttc
    16261 gctgttaaaa aatctctgcc agcgtttttc ctgccagttt aaggtcattg cccccatcgt
    16321 tttggcgttg atcgcctcgc gccagtgact ctctttactg ataccgtagc tctcttcata
    16381 gacaaagagc ttgatatcat caatgtcatc aatatcttcg ccgccggtgg tattgatttg
    16441 cagaaactca tcgcctgaag tgtagtagcg gaaaatctga ctaccgccgc ccagatcgat
    16501 gtggctgaca gcggctaccg taaattcctc acccggcagc gcaatcagca attcatcttc
    16561 cagcaaacga aacgctaacg tgtcgagcgt aaagccacta ttgagatgaa gtcccagcgg
    16621 accgcgagcg atagcaggct tattatcctt gccaaacaga cgctggaaaa aaccagacat
    16681 attttatcct taaaatagtt gccagccttg tagtgaggct ggcaaaaaaa ttactcgccc
    16741 tgttggcgtt gcagtcttgc taatacatcc tgggcgctac ttttattgct accgccaatt
    16801 ccggcttccg ccagcttttc gtcaaggtcg cgaccgtctg cgactttctc caactgtgcg
    16861 gcagcatcca ggcgagcctg acgttctgcc tgacgcgttt gcaggcgttt taaggattct
    16921 gccgctgtcg aaacgctgga agatgcgcca acggtagagg ttgttacagc ctgttgtgca
    16981 cgctgcatgg cttcagtggc tttaacgact tccatttgct gctcaaactg agcgatacgc
    17041 tgcgctgtcg ctgtaactgc tttttccacg ccatcacggg agacttccag attcgacaac
    17101 acttgctctt cagcggtaat gagattctca aggcgcgcga tttcttcagc aacttcgtta
    17161 atcaacgacg gattaacgtt cttgcttaac gcttccagcg cacgggcttc cagactggct
    17221 ttgcgctcac gtaaatcttt cagcttatcg tgacttaatt tcacccgcgc cagcagatcg
    17281 acgcgagatt ttccggcttt atcgagttca gctttagcgt cacgaatatg ctgttccagc
    17341 atgcgcacgc cctgggtttc ttcaatggac tcttccgcct gggagataaa cgatttcccc
    17401 agcgtgaata aactttttaa aattcccata aatacccgtc cttgtaatta gtgtgaatat
    17461 tcttctgtaa tttcagccag atccaatgcg ttatctacca gcgaggctat ctcaagcagg
    17521 atatcttcaa gagaagattt aagcgataac gccccgaaaa caatgtaata ctcttcctgt
    17581 tgcacgctgg agattcctac cgatgacaac ggcatcatct tctgatttct taataagaag
    17641 gtattaaatt catctggatt gctgatacta cttaccggac aaataaaggt ttcgataatc
    17701 atttggcggg aagtaaaaag aatattgatt tgcaaatcgc cataatcatt cattttaata
    17761 attaatgcgt tatcgctatt tgttacatca atattttgtt caggtacagt ttgcagcgcc
    17821 gtcgccagcg ccaacggatt ccatgtcata tattattcca tatagattta gtttaaatat
    17881 taacaaaatg aatatttgca gtacgtaatt atcttaccag ctatagacaa aaaaaaacca
    17941 tccaaatctg gatggctttt cataattcag aggaactagc tgcgctgacg caccgcttca
    18001 aataagcaaa ttccggttgc aaccgaaacg ttcagggaag aaacacttcc tgccattggg
    18061 atgctgatca actcatcgca atgttcacgg gtcaggcgac gcataccttc accttccgcg
    18121 cccatcacca gcgccaggcg tccggtcatt ttgctctgat agagtgtatg atccgcctcg
    18181 cctgccgtac cgacgatcca gatattctct tcctgcaaca tacgcatggt gcgcgcaagg
    18241 ttagtcaccc gaatcagtgg aacgctttct gccgcgccgc aggctacttt tttcgccgtg
    18301 gcgttgagct gtgcggagcg atctttcggc acaatcaccg cgtgaacgcc agcggcgtcc
    18361 gcgctacgca ggcacgcgcc gaggttgtgc ggatcggtta caccgtcgag gatcagcagg
    18421 aacggttgat cgagcgaagc gatcagatcc ggcagatcgt tttcctgata ctgacgtcct
    18481 ggcttcacgc gggcgataat gccctgatgc acggcaccgt cgcttttctc gtcgagatat
    18541 tggcggtttg ccaactggat aaccacgccc tgagactcaa gggcgtgaat cagcggtaac
    18601 agacgtttat cttcacggcc ttttaaaata aagacttcct gaaaacgttc aggggcgcgc
    18661 tccagcaggg cctgcactgc gtggatgccg taaatcattt cgctcattaa tgtactcgtt
    18721 gtttacgttt tgcctgatgc actacgttta tccggcctac atgatctctg caatatattg
    18781 aatttgcgtg attatgtagg ccggataagg cgttcacgcc gcatccggca tgaacaaagc
    18841 gcattttgtc agcaatctaa ccctcttctg tttgaagagg atattgatca ctctgccact
    18901 tttttcttcg ccgcacgctt cgctttggtc gctgcggcta ttttctgcgt tttcgccgat
    18961 ggctttttcg cttttctcgc gtctttcttc gctgctttcg gcttcgtttt tttctcaccg
    19021 cggaaggcgc tgtctggctc aaagtttacc tttttaccga cctgacgacg cttgccgcct
    19081 tttttgcctg catcgccttt tttcgctttc tcacgcgccg ttttaccgac gttgcgcggt
    19141 gcgcgttcgc tggagatcag gctaaagtcg attttgcgct cgtccatatt aaccgcttcg
    19201 acgcgaactt ccacgcgatc gcccaggcga taagtctggc cgctggattc acccatcagg
    19261 cgttgcccta cctggtcaaa gcgatagtag tcattgtcca gcgaagagac atggaccagc
    19321 ccatcaatga acaagtcgtc cagacggacg aagaagccaa agccagtgac gctggaaatt
    19381 acgcctttaa agacgttacc tacctggtcg agcatgaagt cacacttcag ccagtcagcc
    19441 acatcgcgcg ttgcttcgtc ggcacgacgt tccgccatcg aacagtgctg acccagttgc
    19501 agcatctctt ccatcgaata atggtagcca ccggtttcag tggtgttgcc ctgatgcccc
    19561 tgctctttcg ccaacagata cttaatggcg cggtgcagcg tcaggtcagg ataacgacga
    19621 atcggcgaag taaagtgcgc ataggactgc aatgccaggc caaagtgacc acggttttcc
    19681 ggatcgtaaa tcgcctgttt catcgagcgc agcagcatgg tttgcagcat ttctgcatca
    19741 ggacggtcgg caaccgactc cagcagctcc gcgtagtcgc gcggttccgg cttgttaccg
    19801 cccggcagtt ccagccccag ctctgccagc actgaacgga aagaggtaat cgcttcggtg
    19861 ctcggcttgt cgtgaatacg gaacagtgcc ggttctttcg ctttctcaac gaaacgcgcc
    19921 gccgagatat tcgccagaat catgcactct tcaattaact tgtgtgcgtc gttacgctga
    19981 gtctgttcga tacgttcaat acggcgttca gcgttgaaaa tgaacttcgc ttcttcgctc
    20041 tcaaatgaga tcccgccgcg ttcttcacgg gctttatcca gcactttata gaggttatgc
    20101 aactcttcga gatgcttaac cagcggggcg tactgctcgc gcagatcctg atcgccctgc
    20161 agaatatgcc agactttggt gtaggtcaga cgcgcgtgag agctcatcac cgcttcgtag
    20221 aatttgtagc cagtcaggcg gcctttcgac gaaaccgtca tctcgcacac catacacagg
    20281 cggtctacct gcggattgag cgagcacaga ccgttagaga gcacttccgg cagcatcggg
    20341 ataacctgcg aagggaagta caccgacgtg ccacggttac gcgcttctct gtccagcggc
    20401 gttggcggac gcacatagta gctgacgtcg gcaatcgcga cccataaacg ccagccacca
    20461 ccgcgttttt tctcgcagta aaccgcatcg tcaaagtcac gggcgtcttc gccatcaatg
    20521 gtaaccagcg gtaaatcgcg cagatcgaca cggcccgctt ttgcttcttc cggcacttct
    20581 tctttcagcc cggcaacctg ttgctcaaca gcctgcggcc agatgtacgg aatttcatgg
    20641 gtacgcagag cgatatcaac cgccatgccg gtgcccatat tgtcgcccag cacttcgacg
    20701 attttaccca ccgctttggt acggcgagtc gggcgttggg tcagttcgac tacgaccaca
    20761 aagcccatcc gcgcgcccat aatctgatcg ggcgggatta aaatatcgaa gctcagacgg
    20821 ctatcgtcag gaaccacaaa gccgacgccc gcttcggtaa agtagcgacc aacaatctgg
    20881 ctggtttttg gcaccagtac gcggacaata cgcgcttcac gacgaccttt acggtcagcg
    20941 cccagcggct gcgccagcac ctgatcgcca tgaatgcagg ttttcatctg ctcgctggag
    21001 agatacaaat catctttacg cccttcaacc cgcagaaagc cgtagccatc acggtggcca
    21061 ataacggtac ctttcaccag gtcgaggcgt tccggcagcg catagcactg acgacgagtg
    21121 aagaccagtt gaccatcgcg ctccatcgcg cgcaggcggc gacgcaggcc ttcaagctgc
    21181 tcttcgcctt caatgtgcag ttctaccgcc agctcatcac ggctggccgg tttttcacgt
    21241 ttggttaaat gttcgaggat aaattcccga ctagggatgg gattcgcgta tttttcagct
    21301 tcgcgttcct ggaaaggatc ttgtgacatc tcggttcctc cgttgtcatc tctgatgaag
    21361 attttcgtca ctccaccagc aataatttat aaagcggttg attctcttca accaaatcgg
    21421 caagcgtgta gttatccagt tccgtaagaa aactttgcac ggccttagaa agcgcctgtt
    21481 tcaaccgaca ggcaggtgta atgtggcaaa actcactgct gcaattcacc agcgataagg
    21541 gctccagctc gcgcaccaca tcaccaatac gtatcgcact cgccggttta cccaagcgaa
    21601 tgccgccatt ttttccacga acagcagtca cgtagccggc acgactaagt tgattgatta
    21661 ttttgaccat atgattacgg gagacgccgt agacgtcagt cacttcagaa atactggtca
    21721 tccgcccttc tggcaatgac gccatgtaga tcagcgcacg taatccgtaa tcagtgaaac
    21781 tcgttaactg cacatcaacc tcaaaaggga aatcgggaaa aaatacattt atattgatga
    21841 taaaccagcc acaagctgtg tcgctaattt atttcagttc ggggaaggaa aaaagcgagg
    21901 atttaacact gtgtcggata gcgtaatggc ttaaccgaca cagcaatgta tcacactgtt
    21961 agcccagcag gcaaaatatc tgccaggcgt accaaaatta cgcgtcgaac gggtcgcgca
    22021 gaatcatggt ttcagtacga tccggaccgg tagagatgat atcgatcggc acaccggtca
    22081 gctcttcaat acgtttgatg tagttcagcg ccgcctgcgg cagaccgcta cgttctttca
    22141 cgccgaaggt ggattcagac cagcccggca tggtttcgta aatcggctct acacctttcc
    22201 agtcgtcagc tgccagcgga gtggtagtca cttcgcgacc atccggcata cggtaagcca
    22261 cacagagttt cacctctttc aggccatcca gaacgtccag tttagtcagg cagaagccag
    22321 acagggagtt cagctgtacc gcacgacgaa cggcaacggt gtccagccag ccggtacgac
    22381 gacgacgacc cgtagttgcg ccgaattcgt taccctgctt gcagaggaac tcgccagttt
    22441 catcaaacag ttcagtcggg aacggacctg cacccacacg agtggagtaa gctttgagga
    22501 tacccagaac gtaatcaaca taacgcgggc ccaggccgga accggtcgcc acgccaccag
    22561 cagtggtgtt ggaagaagtt acgtacggat aagtaccgtg gtcgatatcc agcagcgtac
    22621 cctgcgcacc ttcaaacatg acgaaatcgc cacgctgacg cgcctggtcg agcaggtcag
    22681 aaacgtcaac caccatagaa gtcaggatgt cggcaacagc catcgtatca tccagaactt
    22741 tctggtaatc aaccgcttca gctttgtagt agttaaccaa ctggaagttg tgatattcca
    22801 tcacttcttt cagtttttca gcgaaggttt ctttgtcgaa aaggtcgcca acacgcagac
    22861 cgcgacgtgc cactttatct tcataagcag gcccgatacc acgaccggtg gtgccgatcg
    22921 ctttcgcgcc acgcgctttc tcacgcgcgt tatcaagcgc aacgtgataa tcaaggatca
    22981 gcggacatgc ttcagacagc agcagacgct cacgaacggg gatgccacgg tcttccagtt
    23041 ctttcatctc tttcatcagc gcggccggag acagcacaac accgttaccg atgatgctgg
    23101 ttacattctc gcggagaata cctgatggaa taagatggag aacggttttt tcaccgttga
    23161 ttacgagagt atggcctgcg ttgtgaccgc cctggtagcg tacaacatat ttagcccgtt
    23221 cagtcagaag atcgacgatc ttacctttac cttcgtcacc ccattgggtg cccagtacga
    23281 cgacgttgtt acccattttt caaaatcacc gtttgcttaa aaatggattc taccatcgct
    23341 ttttcagagt tacagcactt tttgcactca aaaatgacct cgatcagtct gttttttgtt
    23401 cagccaatcg ttttcctcaa catgtagtag accacaacgc ccgcaaccac aagtccaccg
    23461 ccaaaacgac gtaaaatatt atcgggcaaa ttggtcatcg cagagatcat cttcttccat
    23521 gccttcgggt aaagcatcgg ccctaaacct tccagtacca aaaccagggc aagcgccagc
    23581 cagattgtcg aattcattca ttatccttat agaaaaagaa aaccaccgac atcccagagg
    23641 atgcggtggc tttattgacc tgtaccgcag tcgttatatt aacgcgttgc ggaagtcggc
    23701 gtcttcatgt agcggaagaa atcgctatcc gggctcatga ccatcacgtc ctgattgcca
    23761 gagaagctgt tctcataagc acgcaggcta cggatgaatg cgtagaagtc cggatcttta
    23821 ctgaatgcat cagcaaacag tttggctgct tcggcatcgc cttcaccacg catgatacgg
    23881 ccctgacgct cagcttctgc cagcgttctg gtcacttcat agtcggcagt cgcgcgcagt
    23941 ttttccgctt cttcctgacc ttgtgaacgg tgacgacgcg ctaccgcttc acgctcggcg
    24001 cgcatacggt tgtagatcgc ttcagacact tcggtcggca ggttgatctg cttgatacgc
    24061 acatcgacaa cttcaatacc cagcgccgcc atactgttcg ggttgatgac cggaactttg
    24121 cccttcgtct cagccgttac gcgctctgcc gcttcggcaa tggcgttatc tgccgccggg
    24181 gtagtaactt catcttctgt acccgcagaa ccggagttca gcgcgtcacg tacttcgagg
    24241 gtcagacgac cacgggaatc agtgacgata tctttcacgt ccaggcgacc aatttcagaa
    24301 cgcagacggt cagagaactt acgtttcaac agcacttccg cttgcgaaat gtcgccacca
    24361 cccgttgcca ggtagtaacg gctgaaatcg ctgatgcgcc atttgatgta agagtcgacg
    24421 atcaggtctt tcttctcttt ggtcacaaag cggtcggcct ggttgtccat ggtctgaata
    24481 cgtgcgtcga gcattttcac cgtttcaatg aacggtatct tgaaatgcag acccggctca
    24541 taaaccagag gtttgttgtc atcgtcacgc agtaccttac caaaacgcag cgtaataccg
    24601 cgctcacctt ctttgacgac aaagacagac atgtaaagca ctaccagcac gatgatgata
    24661 atcgcgataa ctgacttacg catcgttatt ccccctgacg ctggtagtcg ttacgctgcg
    24721 cgttggcgcg gcgttggtcc ataatatcgc cctgactggt ggacgacgtg ttgcttgctc
    24781 cactggttgt ggaggaagag gctggcggca gacgcagcag attgctggca ccgttatcgc
    24841 tcttcgccgc aggggcgtta ccacctttca gcatctggtc taacggcaga accatcaggt
    24901 tgccaccttt atcgttaacc agcactttgc gggtgttacc caacactttt tccatcgtct
    24961 cgatatacag acgctcgcga gtaatttccg gcgcggcttt atattccggc agaagtttag
    25021 caaagcgcgc cacttcaccc tgagcttcca ggatggtctg ggccttgtac gcacgcgcct
    25081 cttcgaggat acgttgcgcc tgaccgttcg cacgcggctg aacttcgttg gtatacgctt
    25141 ctgcttcacg aatgtattgc tgttcgtttt cacgcgcggc aatcgcatcg tcaaacgccg
    25201 cttttacttc ttccggcgga cgagcagcct ggaagttgac gtccagcagc gtgataccca
    25261 tgtcatacgg gcggatagtc tcttccagtt cgcgctgagt atcgctacga atcacggtac
    25321 gaccttccgt cagaatgcgg tccatggtgt atttaccgat aactccacgc agggcgctgt
    25381 cggtagcctg acgcaggctg tcatccgggc tggtcacgct atacagatat ttttccggat
    25441 tggtgacgcg gtactgcacg ttcatctcaa cgcgcactac gttctcgtcc gacgtcagca
    25501 tcacaccaga agcggccagt tcacgcacgg cttccacgtt caccggtttg acttcgtcga
    25561 taaacgtcgg tttccagttc agacccggct caaccagatg gctgaattta ccaaagcgtg
    25621 ttaccacgcc acgttcggct tctttaatgg tatagaaacc actggccgcc cagataatga
    25681 caatcgctgc cgctgcgatg gtaacgacac gaccgccaag ctgcgggcgc gggccttgcg
    25741 atgaactgcc accgccagat ccggtgcctt taccgccgcc cagaccaccg agctttttac
    25801 tcagtttgcg gaagatatca tctaaatcag gtggcccttg atcgcgaccg cctttgtttc
    25861 catttccctc agagttgccg ccaggtttgc tgcttcccca cgggtcgcgg tcttgtccgt
    25921 tattaccggg ctgattccac gccatgtttg tgctccatat ttgttatgcg gtgatccctg
    25981 ttgtctttcg acgccagagg atatgactcc acgcttcaga cgctacgccg ttagatcagg
    26041 taatcgatca acgccggttc ttgtttacag aggcgacgcc agtcaacgat cggcatacga
    26101 acttgcagac ttacgctgcc gtcctcctcc atccactctt tttctattgc ctgaagctga
    26161 taaaaacgac ttctcagacg cccttcctgc ggtggcagac gcaatgtatg ctgcgccacc
    26221 tcgccggaaa gccgctccgt caaagcctga aaaagctgtg gtatccccgc tccggtctgt
    26281 gcggaaagcc agacacggat cggtttgttc tcttcgtccc gatcaatacg cggttcgaaa
    26341 tcttccagca tatcgatctt gttcatcacc agcagggttg ggatctcgtg agcgtcgatc
    26401 tcttcaagaa ccgtattcac cgcttcgatg ttttcttgta cacgcacatc cgccgcatca
    26461 atgacgtgca gcagtaatgt ggcttgccgc gtctcttgta acgtggcttt aaatgctgcc
    26521 accagatcgt gcggcaggtg gcgaataaac cctacggtat ctgcaagtac ggtttcaccg
    26581 acatctgcaa cgtcaatacg ccgcaacgtc gggtcgaggg tggcaaacaa ctggtctgcc
    26641 gcgtagaccc gcgcttcggt gatgcgattg aaaagggtag atttaccggc gttggtatat
    26701 cccaccagcg aaacagtagg aacgtcggct ttgatacgcg attgccgccc ctgctcacgc
    26761 tgcttttcaa ctctttccag gcgcgactgt atctgcacga tgcgattacg caacaaacga
    26821 cggtcggttt cgagctgggt ttcacccgga ccacgcaaac ctatcccgcc tttctgtctt
    26881 tcaaggtggg tccagccacg caccaggcgc gtagccagat ggcgcagctg cgccagctca
    26941 acctgcaact taccctcatg ggtacgcgca cgttgggcga aaatatctaa aataaggccg
    27001 gtgcggtcga taacacgaca ctcgcacaaa cgctccaggt tacgctcttg cgccgggctc
    27061 agggcatggt caaaaagaac gaccgaagca cccgtcgctt tgacagcttc cgcaatttca
    27121 actgctttac cttcacctac aaaatacttt gggtgcggcg ctttacggct accggtaatc
    27181 acctgcaatg cttcgacacc ggcggaagag accagagatt caaactcctg gaggtcttcc
    27241 atatctttgt cttgcgtaaa atagatgtgt accagtaccg cctgctcacc agcatcataa
    27301 cggtcaaaca agcgtataac cctctaaata gatcagcggg gaacgcagga tcgctggctc
    27361 cccgtgtaaa aaaaacagcc cgaaacctta ttcggtttct tcgctgtcct gttgcgcgga
    27421 agtattctgc gcgctgctac catgatggta gttactgctg gtaccgccac cggcattgtt
    27481 actgtgatga gaaaccgggc gagacgggac aacagtagaa atcgcgtgct tgtaaaccat
    27541 ctggctgacc gtgtttttca acaggatcac gaactgatca aaagactcga tttgcccttg
    27601 cagcttaata ccattcacca aataaataga aactggaaca cgttcccgac gcagtgcgtt
    27661 caggaacgga tcttgtaaag attgcccctt agccattctc tcttttcctt atatgcttat
    27721 ttgtactttg aacctttcga ttctgaaaaa attgcgcacg atacgtctca attgtacaca
    27781 ttcagcctgc gatagcacca acaacctgta atacttcgtc acgcgcctgt tctggttttt
    27841 cactatcaag ccagtgaacc ccttcccaac cacgcagcca ggttatctgc cgcttcgcca
    27901 actgtctcgt ggcgcaaaca cctcgataaa ccatttcatc gtatgagatt tcgccttcaa
    27961 ggtaagacca catctggcga taacccacgc aacgaatgga aggcaagtcc gtatgcaaat
    28021 ctcctcgggc aaaaagcgcc cggacttctg cttcaaaacc tgaagccaac atctgatgaa
    28081 aacgctgctc aatgcgttga tggagcagtt cacggctcgc cggggcgatg gcgaactgat
    28141 gcacctgata cggtagagcg tctcctgacg tttgcgtcag ttccgttaaa gttttacccg
    28201 aaatgaaaaa aacttccagt gcccgggaaa gcctttgtgg atcatttgga tgaatccttg
    28261 ctgccgcaac cggatcgatc tcctgaagtt gacgatgcaa tgactcccaa ccttgctctg
    28321 ccgcctgttg ctcaattctg gcccgtactt ccgggtctgc cgacggtagc ggcgacaacc
    28381 cttccagcaa tgccttgaaa tacaacatcg taccgcccac taacagtggg atccgccccg
    28441 ccgcggtgat atcggccatt tccgccagcg catcgcggcg aaaatcagca gccgagtaag
    28501 cctgcgacgg atcgcgaata tccagcaatc ggtgcggcgc ggcgagtaac tcttcagcgt
    28561 tcggcttcgc cgtcccgata tccatccctt tgtaaataag ggcagaatca acgcttatca
    28621 actctactgg taaaatttta cgcagctcaa tggctaacgc cgttttaccg gaggccgtcg
    28681 gccccatcaa aaaaatcgcc ttaggcaggc tcgccttact gatatcactc atctttcagg
    28741 gcttttatcg ccggatgtaa atcaacagat tgtaacagac cacccggcgg cgttttcaca
    28801 agttgcggac ataaccgttc cacgtccgcc agcagggtta tggcctgtgc cattgaccac
    28861 tgcgcatgtt cgctcatcag atttcgtgca atccactgcg caatattgcc aggttcgaat
    28921 acggactgct tcgccaggta gcctatcagt tcaggaatca agatttgtaa attttgttgg
    28981 cgtaagggta aaggcacggc cctgatggtc acatgctgtg catctgactg gaaatcaata
    29041 cccaattccg ccagggcaga ctgcgctttt tctaatgccg atttttcttc gccagaaact
    29101 tttagccgca acggaatcag caacggctgg gcgcaaacgg gcgcttcacc cggcgtcagt
    29161 tgtacctgac gcagccaacg ttctgccact ggcaaggcta aaagtgaaat gttgccgtcg
    29221 cgctccagca acgcacagtc ggaatggacg atagtcagta cccgaccaaa actctgactg
    29281 ttcgccgcaa gtgcaggttc atgcggttct ggcgcttttg gtttttgcat cggcgcgggc
    29341 gtttgcaaaa gctggcgata cacttcacct tgctgtttct ggtaacctgg ttgcgcattc
    29401 ggccaggggg cagccggacg actgcctgat gctggcgcag gagtgtagcg cggagctact
    29461 ggctcacgaa ctgccggttc agcaaagtga ttgcgccccg ccgccacgcg gttttccggg
    29521 atggcacgcg gtgtgggttg tggttcatcg tccagcggta gcggcgtttc cagctgctgt
    29581 tgtagcacgc tcagcacgcc ctgatagata aagtcatgca ccaggcgcga ctgatggaaa
    29641 cgcacttcgt gtttggcggg gtgtacgttg acgtccacct gatgcgggtc gatctccaga
    29701 tacaacacaa acgccggttg ttgatcggcc cccagtttgt cttcgcaggc ctggcggatc
    29761 gcgtgattga tcaggcgatc gcgcatcatg cgaccattca cgtagcaata ctgaatttcc
    29821 gccagtgcgg gcgtggtgtg atttggatcg gccacccagc cgcgcagcgt gagatcgccg
    29881 tgttgccatt caatcgccag cgcttgttcg agaaaagcgg tgccacaaat cgcgcctaag
    29941 cgccgttctt tttgcccgcc ttccggcact gcgcggtact ggcgtacgat tttgccgtta
    30001 tgcgacaggt tgatcgtgac gtcgaaacgc gccagtgcga tgcggcggat gatttcatcg
    30061 atatggttaa attcggtttt ctcggtgcgc aggaatttgc gccgcgccgg ggtgttgtag
    30121 aacaggtcca gtacctccag cgtcgttccc accggatgcg ccgccggttt aaccgtcacg
    30181 tccatatcgc gcccttcggc ataggcctgc caggcttcct gctgttctgc ggtgcgtgaa
    30241 gtgagcgtca ggcgggaaac cgaactgata ctcgccagcg cctcaccgcg aaagcccagg
    30301 ctgataatgg cttcgagatc gtccagagag gcgattttac tggtggcatg acgcgccagc
    30361 gccagcgcca gctcgtcttt tttgataccg cagccgttat cacgaatgcg gataagtttc
    30421 gccccaccgc gttcgatatc aatatcgata cgcgtcgcac ctgcatcgag gctgttttcc
    30481 accagttctt tgactaccga cgcaggtcgc tcgaccacct cacctgcggc aatctggttc
    30541 gccagttgtg gcggtaagac ctgaattggc atcaatcgtc cttagtttgg cagcgtacga
    30601 tctggcgtcg tcaccgtact ggcagtttgt gccgttgcac cctgcggcgc agattgcatc
    30661 ggatgcgcaa ggaaataatt gcgcagacct ttataaatgg cttctgccag ctgttgttgg
    30721 taatcgtcgc tcgccagcaa acgttcttcg ctgttgttgc tgataaaacc ggtttcgacc
    30781 agtactgatg ggatatccgg cgaacgcaga acgccaaggc tggcgtgttc tggtcgacgt
    30841 ttatgaattt cgccaatgcg ttgcaactga ctgatcatac tggtcgctac atcataccct
    30901 acccgctggg aatgaccgaa ctgtaaatcc agcacagcct ggcttaaata ggggtcagac
    30961 tgactgttcg ccagcacatc gcccgctccg cccagtagct ccgactgttt ctcgtgctgc
    31021 tccagccagc tggccatttc gctgttggcg cgacggttag agagcaccca tacggaagcg
    31081 ccagtcgcac tgcggttcgg tgcggcatca gcgtgaatcg acacgaggaa attggcgttt
    31141 tgcttacgtg ccacatcgct gcgccccatc accgagataa agtaatcccc gtcacgggtt
    31201 aaaacgccct taaacatcgg atcgtcattg agcaaagtac gcaatttacg cgcgatggcg
    31261 atggtgacat ttttctcccg cgtaccgccg gggccgatag cgccagggtc ctgaccgccg
    31321 tgtccggcat caatagcgat gataatttta tcgccagtgt tagccgtcgc gcgcgctgcc
    31381 ggacgcgtta ccgtattact gctgataaca cccgtagtgc ggttactttc cgttttaaac
    31441 ggattgcgcg ccggttcgct gacgcgcggt gcgacaaccg taggcgtttc aacgcgtttc
    31501 gcaaccacag gcggcggagg aggcggtggc ggcgcatcgg cgttaatcgt aaagacgaca
    31561 gtgtaattgc tgccattctg ccgcttcacc gcttcggttt taccattttc ggtaagatcg
    31621 accaccagcc gtagcgtttg tgcatcttta ggcgttccgg agcgaatcgc cttcaccaga
    31681 ttattaccgc tgaacaacaa cggcagtccc tgaatcacgc ccgtttgttt gatatcgagc
    31741 gccacggtgc gtttgctttg atggctaaac gcataatcag gatcgccaat aaaactcaac
    31801 gttatccgcg cctgttgatt accgttagaa acctgaatat cagagagcgt cgcggcaccc
    31861 acctgcgcgc acagcagcag caacgtcgct accaaccaat ttctgatgcg atacatcatc
    31921 ccgccacctt tcaaaggtta accggctaaa cgcgccagca acaattcacc cgcagaggaa
    31981 accgcactca cgcgcgcctc acggccttgt gcctgataat caatgtgtat ttcgacatcc
    32041 gggtcaggaa gaacacctgt accttgttgt ggccactcca ccaggcagat ggcatcgttg
    32101 gcaaaatagt cgcggatccc cataaactcc agctcctcgg gatcggcaag gcggtacaaa
    32161 tcaaagtgat aaaccattaa gttgtcgagc gtatagggtt cgaccagcgt ataggtcggg
    32221 cttttgacat taccctgatg acccaaagcc tgcaaaaagc cccggctaaa ggtggtttta
    32281 cctgcgccta aatcgccata cagatagatg acggttgcac catcgcaggc tttggctacc
    32341 cgctcgccca ggtctaaggt tgcctgctca tcagggagcg gaattactcg attcatcatg
    32401 gtttttttca gtcacttccg ggttaacaat acgctgtagc gtggaaaaga gatcggttgc
    32461 cagcatcccg cgcgttccaa aacgcgccgc cagtacgtca gctgccgcac cgtgcgcgac
    32521 acagcctgca caggctgcat catacggcga cagtttttgc ccaagcaatg cgccaataat
    32581 accagagagc acatcgccca tgccgccgct tgccatgcct gcatttccgg catcaataat
    32641 gcctaaagcg tcaggatggg cggcgaccac ggttccggca cctttcagca ccgctacgcc
    32701 gccataacgt tgtaccagac gtttggcgca atgtaagcgg tcactttcaa tttcagcgac
    32761 ggaacagcct aacaaccgtg cggcctcgcc aggatgcggc gtgatcacgc gattgtgacg
    32821 cttatcggga ttgattgcca gcaggttcaa tgcatcggca tcccacaaca tcggtttgcg
    32881 aaaattctca actttttgca gggctttttt cccccactct tgctggccca gaccgggacc
    32941 aatcaccacc acatcggccc attccaggct ttcggtaaga gagtccatag tcagttcatg
    33001 caccatcaat tctggtcgtg cagtcagcag cgacgcaatg ttctcactgc gggtcagtac
    33061 tcggactaaa ccagcaccag cacgcagcgc cgcttccccc gtcatacgaa tagcccccgc
    33121 cgtgccgtga tcgccaccga taatcaccag ccgcccgtga tcgcctttat gcgaagtcgg
    33181 gcgacgcggt ttcagccagt gagaaagttg ttctgccgaa aaccgctgaa ttttcgtctc
    33241 ctgacctgcc agccaactat ccaaccccag tgagtcaaaa tgcagttgtc cggtaacatc
    33301 ccgcgctttt ccagtgagca agcctggttt cagcgcaata aaagtgatgg tgtgatcggc
    33361 gttgatcacc gcacctggcg tagcgccggt ttcagccagc aggccggaag ggatatcaac
    33421 cgccacaatc ggcgcaggat gggaattagc gtggtcgatt aactggctaa tggattcgcg
    33481 gggcgcttgc tgcaagccgg taccgagcag cgcatcaaca atcagatcta ccgattcggg
    33541 ccagacaata ttcgaagcat ggatctcgcc acccgcgttt aaccatgctt cgcgtgccag
    33601 cgcggcctct tccggcaacg gtttgtcgct atcctgggcc aacaacgtga cctcaatgcc
    33661 gaccgctttg gccagtcgcg cgaccacgta gccatcgccg ccgttattac catgaccgca
    33721 cagcaccagc cagtggcggg cgtcaggata cgccgaacga cacacctgga atgcggcctc
    33781 gccagcgcga agcatcagct catagagtgt gagccccagc acatctgccg cctcgcgttc
    33841 tccgcggcgg atatcgtcgg cgtaccagac ggtgtgtggt atacttacgg ggtttttctt
    33901 cattgtatgg tccgtcatgt cagagcccct cgatctcaat cagttagcgc aaaaaattaa
    33961 acagtggggg ctggaactgg gctttcagca ggtaggtatt accgataccg atctcagcga
    34021 gtccgagccc aaactgcaag catggctgga caaacaatac cacggcgaaa tggactggat
    34081 ggcacgtcac ggtatgctgc gcgctcgccc ccatgagtta ttgcccggta cgctgcgcgt
    34141 gatcagcgtg cggatgaatt accttcctgc taacgccgca tttgccagca cgctgaaaaa
    34201 ccccaaactc ggctatgtta gccgttatgc gctgggccgt gactatcaca aacttctgcg
    34261 caaccgactc aaaaagctgg gcgagatgat tcagcaacat tgtgtttcgc tgaattttag
    34321 accgtttgtc gattctgcgc ctattctcga gcgcccgtta gctgaaaaag ctgggctcgg
    34381 ctggacaggt aagcactcac ttatcctcaa tcgcgaggcc ggttcgttct tctttttagg
    34441 cgaattgctg gtcgatattc cgctgcccgt ggatcaacca gtcgaggaag gatgcggcaa
    34501 atgcgtggcc tgtatgacga tttgcccgac cggtgccatc gtcgagccat ataccgtcga
    34561 tgctcgccgc tgtatctctt atctcaccat cgaactggaa ggggcgatcc cggaagagtt
    34621 gcgaccgtta atgggaaacc gtatttacgg ttgcgatgac tgccagctta tctgcccgtg
    34681 gaatcgctat tcacaactca ccacagaaga ggatttcagc ccgcgtaagc cgctacacgc
    34741 accggaactc attgagttat tcgcctggag cgaagagaag tttttaaaag tcacggaagg
    34801 atcggcgatt cgtcgtattg gtcacctgcg ttggctgcgt aatatcgccg tagcattagg
    34861 caatgcgccc tgggatgaaa cgattctggc ggcgctggag agtcgcaaag gtgagcaccc
    34921 acttcttgat gagcacatag cgtgggcgat tgcgcagcaa atcgagagac gaaatgcgtg
    34981 catagtcgaa gtgcaattgc cgaaaaaaca gcgtctggtt cgggtaattg aaaaagggtt
    35041 accgcgtgac gcctgattca tccacagctt gtgaataaaa ataaaaacgc attgcaattc
    35101 aagacgaata aaatcgtcaa gtgatcgact cgacaaaacg gaatcttaat ttttaactta
    35161 tctatcaata ggttaaaaaa atactattca ccgtgcgaag tttctttgaa attaagatta
    35221 cagcctgagg ctgtggataa ctctgtttac aagagtttgt cagagacaac aaaaaccagc
    35281 aataacgcat cgcgtcgctg tggatatttt attgagagaa gaatttggag cgggaaacga
    35341 gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga gctattcccg
    35401 cgtactactt aattttgctt tcgtaatttt cgcatttctg cgtcgttacg ggaggcgcat
    35461 tatacgagaa atctttccct ctgcaacccc cctaaaaaat tttttttgag tttttatctc
    35521 aagtgctgaa attatcagca aggcgttcaa tttagcgtca aaatcacgcc gccgcaagtg
    35581 gattccgacg cttacgtgac caggaaaaat tttacagctt gataaaatgc tcgcggtagt
    35641 aagccagctc cgccaccgat tcacggatat catccatcgc ctgatgcgtc ccctgcttgg
    35701 taaaaccatc cagaatttcc ggcttccagc ggcgcgccag ctctttcagg gtgctgacat
    35761 cgagataacg gtagtggaag taggcttcca gctccggcat gtatttaaac aggaagcgac
    35821 gatcctggcc gatgctgtta ccgcaaatcg gcgattttcc cgcaggcacc cactgtttta
    35881 agaattcgag cgttgccagt tcagcttcac gatcgcccat cgtgctcgct ttcacgcgct
    35941 ctaccagccc gctggcggta tgggtgcgca cgttccagtc atccatcagc gccagctgtt
    36001 catcggactg gtgtactgca atggtcggcc cttctgccag aatattcagg ttggcatcgg
    36061 tcaccagcgt ggcaatctca ataatgcgat cgcgctcggg atccagaccg gtcatctcaa
    36121 gatcgatcca aatcaggttg ttttcattgg cactcatgct attttccacc cattcacagt
    36181 gactatattg atccacaatt gcgtgtatca tagatgtttt gcccatcagg ggcgaccagg
    36241 agtctgtacg attgagtaaa aataaactct ccaaaggcca gcagcgccgc gtgaacgcca
    36301 atcaccagcg tcgtcttaaa acgtctaagg agaagcccga ctacgacgac aatctgtttg
    36361 gcgagcctga tgaaggtatc gtcatcagcc gctttggtat gcacgctgat gtggaatccg
    36421 ccgatggcga cgttcaccgc tgcaatattc gccgtaccat ccgttcgctg gtgaccggcg
    36481 accgcgtagt ctggcgtccg ggtaaaccgg cggcggaagg cgtaaatgtc aaagggatcg
    36541 tggaagcggt gcatgagcgc acctcggtgt tgacgcgtcc ggatttctac gacggcgtga
    36601 aacctattgc cgccaacatc gaccagattg tcattgtctc cgccattttg ccggagctgt
    36661 cactcaatat tatcgaccgt tacctggtgg cctgcgaaac cttgcagatt gagccgatta
    36721 ttgtgctcaa caagatagac ctgctggacg acgaaggcat ggcgttcgtc aacgaacaga
    36781 tggatatcta ccgcaatatc ggttatcgcg tattgatggt ttccagccat actcaggatg
    36841 ggctaaaacc gctggaagag gcgttgaccg ggcgcatcag catttttgcc gggcagtctg
    36901 gcgtcggcaa atccagcctg ctgaacgcgt tgctggggct gcaaaaagag atcctgacca
    36961 acgatgtctc tgacaactcg gggcttggtc agcacaccac gaccgccgct cggctgtatc
    37021 acttcccgca cggcggtgat gtgattgatt cccctggtgt gcgtgagttc ggcctctggc
    37081 acctggagcc ggaacaaatc actcagggct ttgtcgaatt ccatgactat ttaggtctgt
    37141 gtaaatatcg cgattgcaaa cacgataccg atccgggctg cgctatccgg gaagcggttg
    37201 aggaagggaa aatcgcggaa acccgtttcg aaaactatca ccgtattctg gaaagcatgg
    37261 cgcaggtaaa aacgcgtaaa aacttttctg atacggatga ctgacaatta agctaaccgt
    37321 cgttagaatc gtcccctttt tttcaggatc cggcatgtag gccggatcag gaacgacaaa
    37381 acaatggcct ggaggctacc ttgttaaatt catttaaact ttcgctacag tacattctgc
    37441 cgaaactatg gcttactcgc ctggcgggtt ggggcgcaag caagcgggca ggatggctga
    37501 caaaactggt tatcgatctg ttcgttaaat actacaaggt cgacatgaaa gaggcgcaaa
    37561 agccggacac cgccagctac cgcaccttta acgaattctt tgtccgtccg ctgcgtgacg
    37621 aagtacgccc aatcgatacc gatccgaatg tcctggtcat gcctgccgat ggcgttatca
    37681 gccagttggg taaaatcgaa gaagataaaa tcctgcaagc caaaggccac aactacagcc
    37741 tcgaagccct gctggcaggc aactatctga tggcggacct gttccgcaac ggtacgtttg
    37801 tgaccactta cctctccccg cgtgactacc accgcgtaca catgccgtgc aacggtattc
    37861 tgcgtgagat gatctacgtg ccgggcgatc tcttctccgt taaccatctc acggctcaga
    37921 acgtgccgaa tctgtttgcc cgtaacgaac gcgtgatctg cctgttcgat accgaatttg
    37981 gcccaatggc gcagattctg gtcggtgcga cgattgtagg cagcattgag actgtctggg
    38041 cgggcaccat tacgccgccg cgcgaaggca tcatcaagcg ctggacctgg cctgccgggg
    38101 aaaacgacgg ttctgtggcg ctgctgaaag gccaggaaat gggtcgcttt aaacttggct
    38161 ctaccgttat caacctgttt gcaccgggta aagtgaatct ggttgagcaa ctggaaagcc
    38221 tgtctgtcac gaaaattggc cagccgctgg cagtatctac cgaaaccttt gttacgccag
    38281 acgctgaacc agccccactt cctgctgaag agatcgaagc agaacacgac gccagcccat
    38341 tggttgacga taaaaaagac caggtctaat ccatcaaagg aaacgctgac gtgcgcctga
    38401 ttatcacttt tctgatggcc tggtgcctca gttggggggc gtacgccgcg acggcccccg
    38461 atagcaaaca aatcacccag gaactggagc aggcaaaagc ggcgaaaccc gcgcagccag
    38521 aagtcataga ggcactccag tctgccttaa atgcgcttga ggaacgaaaa ggttcccttg
    38581 agcgcatcaa gcaatatcaa gaagttattg ataattatcc gaaactctcc gctactctgc
    38641 gcgcacaatt aaacaacatg cgtgacgagc cgcgcagcgt gtcgccgggg atgtctaccg
    38701 acgcgctgaa tcaggaaatt ctccaggtca gcagtcagtt gctggataaa agccgtcagg
    38761 cccagcaaga gcaggagcgc gcccgcgaga ttgccgattc gctgaatcaa ctgccgcaac
    38821 agcaaaccga cgcccgccgt cagttaaacg agatcgagcg ccgcctggga acgcttaccg
    38881 gcaatactcc gctcaatcag gcacaaaatt tcgcgttgca gtctgactct gcacgtctta
    38941 aggcgctcgt tgatgaactg gagctggcgc agctgtctgc caataaccgc caggaattag
    39001 cgcgcttgcg ctctgagctg gctgaaaaag agagccagca actggatgcg tatttgcagg
    39061 ccttgcgtaa tcaattgaac agccaacgcc agcttgaggc agagcgggcg ctggaaagta
    39121 ccgaattact agcagaaaac agtgccgatt tgccgaaaga tatcgtcgcg caattcaaaa
    39181 ttaaccgcga actatcggca gcgctgaatc aacaggcgca gcggatggat ctcgttgcct
    39241 cgcaacagcg tcaggctgcc agccagacgt tacaggtccg gcaggcgttg aatacgctgc
    39301 gtgaacagtc gcaatggctg ggatcgtcca atctgctcgg tgaagcgttg cgggcgcagg
    39361 tggcacggct gccggaaatg ccgaaaccac aacagcttga taccgaaatg gcgcagttgc
    39421 gtgtgcaacg gttacgttat gaggatctgc ttaataaaca gccgctgcta cggcaaattc
    39481 atcaggccga cggtcagccg ctgaccgccg agcaaaaccg tattctggaa gcacaactac
    39541 gcactcagcg tgagttgctg aactcattgt tgcagggtgg cgacacacta ctgctggaac
    39601 tgaccaagct gaaagtctct aacgggcaac tggaggatgc gctgaaagag gtgaacgaag
    39661 cgacgcaccg ctatctgttc tggacctctg acgtgcgccc gatgaccatc gcctggccgc
    39721 tggaaatcgc ccaggatctg cgtcgtctca tttcgctgga caccttcagt cagttgggca
    39781 aagccagtgt gatgatgctg accagcaaag agacgatttt gccgctgttt ggcgcgttga
    39841 ttctggtcgg ttgcagtatt tactcgcgcc gctattttac ccgttttctc gaacgttcgg
    39901 cggcgaaagt cggcaaagtg actcaggatc acttctggct gacgttacgc actcttttct
    39961 ggtcgattct cgtcgcgtca ccgctgccgg tgctgtggat gacgctgggt tacggcttgc
    40021 gcgaggcgtg gccttatccg ctggcggtcg cgattggcga tggtgtaacg gccaccgtgc
    40081 cgctgctgtg ggtagtgatg atttgcgcca cctttgcccg cccgaacggc ttgtttatcg
    40141 ctcattttgg ctggccgcgc gaacgtgttt cccgtgggat gcgctactac ctgatgagca
    40201 tcgggcttat tgtgccactg attatggcgc tgatgatgtt cgataacctc gacgaccgcg
    40261 aattttccgg ttcgctggga cggctttgct ttatcctcat ttgcggtgcg ctggcggtgg
    40321 tcaccctcag cctgaaaaag gccgggatac cgctgtatct caacaaagag ggcagcggcg
    40381 acaatattac caaccatatg ttgtggaaca tgatgattgg cgcgccactg gttgccattc
    40441 tggcgtcggc ggtgggttat ctggcaacgg cacaggcgct gttagcgagg cttgaaacct
    40501 cggttgccat ctggttcctg ctactggtgg tttatcatgt tatccgccgc tggatgctga
    40561 tccagcgccg caggctggcg tttgaccggg cgaagcatcg ccgggcagag atgttagcgc
    40621 aacgtgcacg tggcgaagag gaagcgcatc atcacagtag cccggaagga gcaattgaag
    40681 tcgatgaaag cgaagtcgat ctcgatgcca ttagtgcgca atccttgcgg ctggtgcgct
    40741 caattttgat gttgatcgcc ctgctttcgg tcattgtgct gtggtcagaa atccattccg
    40801 ctttcggctt cctcgaaaat atttcgctgt gggatgtcac ttccacggta cagggcgtgg
    40861 aaagtctgga gccgattacc ctcggtgcgg ggctgattgc cattctggtg tttatcatca
    40921 ccacgcagct ggtgcgcaat ctgcccgcgc tgctggaact ggcgattttg cagcacctgg
    40981 atttaacgcc gggtaccggc tacgccatca ccaccatcac caaatatctg ctgatgctga
    41041 ttggcgggct ggtcggcttc tcaatgattg gtattgagtg gtcgaaattg cagtggctgg
    41101 tcgccgcgct cggtgttggt ctcggttttg gtttgcagga aattttcgcc aactttatct
    41161 ctggcctgat catcctgttc gaaaaaccga ttcgcattgg cgatacggta acgatccgcg
    41221 atcttactgg tagcgtgaca aaaattaaca cccgcgccac caccatcagc gactgggacc
    41281 gtaaagagat aatcgtgccg aacaaggcgt ttattaccga gcagtttatc aactggtcgc
    41341 tctctgactc ggtcacgcgc gtggtgttga cgattccggc ccctgccgat gccaacagtg
    41401 aagaagtgac ggaaatcctg ctcaccgcag cgcgtcgctg ctcgctggtg atcgacaacc
    41461 cggcaccgga agtcttcctg gtggatctgc aacaggggat tcagattttc gagctgcgta
    41521 tttacgccgc tgagatgggt caccgtatgc cgctacgcca tgagatccac cagctgattc
    41581 tggctggctt ccatgcccac ggtatcgata tgccattccc gcccttccag atgcgtctgg
    41641 aaagcctcaa cggtaaacaa acggggagaa cgctgacgtc tgcgggcaaa ggtcgtcagg
    41701 cgggaagttt gtaatgcatg aagctgccaa atgatgtcaa cgtggctcat ttagcagctt
    41761 aacatcatct tcagttatca ccggccctgg agagtatcat ccatacgcat tacttcctga
    41821 caactcatcg ccggagagac gatgagttgc aggatttcat atgatcagtc gagagctaac
    41881 tcaggtttat tcgccatgcg actgcaatag ttctggtaaa tcgccatcgc taacagcgag
    41941 aagaagacag ggccgccaat catccacaat gtgctgtccc agtctccggc ttcaaccaca
    42001 ggttgaatga tggtgaagac gttcgcaaat gtcaccacca gtacgacaac cactgtcgca
    42061 atcattgccg acatacgcgt tttgaaaatc acaaatggtc tgtcgagatc ctgacgtgct
    42121 ttaaagaatg ggaaagccag cgcgaggaac aggtaaggaa gcgtcataga cacgttcgcc
    42181 atcagcgtca gcttgttaaa gaacgccgat gcggtaccgc caccaaacga aaccagcagg
    42241 atgaagacag taaccaaccc gcactgcatc cacatggcga tagacggcat ccccatcgca
    42301 ttcaggcgcg tcatcggttc cggccacaac gctttcggcg tcccctggat gatggctttc
    42361 agcggtgaat agcaaagcgt aaagaacgca ccggtataag cgaggaacat cgaaagtccg
    42421 gtaatacgcg caaaccatac gcccagcgac aatgacgctt caggtgacaa atgcagtgcg
    42481 ttacccagcg tcaccccgag gctcttcatc agcacatagg taatattgcc aaggttaacg
    42541 gaaccattac ttaatacctg ctgccagttt gtgctgacgc cccataaaaa tattgccagc
    42601 gaataaccga ttgaaataac aatagcggca aaaacaatac ctttggcaaa gttcttttct
    42661 ggattttccg ttttatcgac cagaccacca accgcttcaa ttccgccata ggcaaaaata
    42721 gcaaatacca caaacgatag cattgccaga ccggactgat aacctgggtt cggtgatgca
    42781 aggaaattaa tatcctgcgc gaaatgccca ccatttaata acaaaatagt aatgcttact
    42841 aacagcaata ctaaattcag acacattact gcaatacccc ccaccgcagt aatgcgggca
    42901 attttattaa tcccctttga agcaacgacg gtgaccagaa tcatccatgc cactgccagc
    42961 agaccaacca cctgcgtagg ctccagtccg gcaatacgcc agtgctgggt catgtcgcta
    43021 ccatagagga atgttgagaa cggtacccaa actttcgcgg aggtgctcac catccagatg
    43081 atataagagg aaaaccacat aaacgtacca atgaaggcaa aacgtggtcc gacactatta
    43141 ttcatccagg aatagatacc gccttcttct ttgcgataag cagctcccat ttcagccatc
    43201 attaaggcga atggaataaa gaataacaat gcagaaaata tataaaaggg aatcgcacta
    43261 taacccatta agtaataagc cgatgggcta ttggcaaatc caaatacgga agtaaagatc
    43321 atcaatatga gtcctatcag actcatcttt tttatcgtgt gaggcatgaa accatccttc
    43381 atttcagcgc gtgtataaaa cgcgcccact gaactgcact gcccaacatt gagcgggcag
    43441 cgtaaaaacc aatacagaat tgatacctgg atgatatcaa atatccagtc ttaaattgtg
    43501 gcctttagcg gaaaaatgaa aacatatgct acagaatggc gcttatcacg ccattcttcg
    43561 ctgttaattc agtaattttt cagaattatg cccggtcaac gctaaaggcg atgacttcag
    43621 ccagtgtctc cgcacccagc gccaacatca ccagacgatc aacgcctaat gccacgccgg
    43681 aacagtcagg cataccgact ttcaaggctt caatcagatt ctggtcaatg gggtgctgcg
    43741 gcaaaccgcg cgccgcgcgc ttacggttat cttgttcaaa gcgttgttgc tgctcacggg
    43801 catccgtcaa ttcatggaaa ccattcgcca gctcaatacc tttataataa acctcaaagc
    43861 gttcagcgac ccgatgatct tcggtactga tttgcgccag tgatgcctgg ctggctggaa
    43921 agtggtacac aaaggtcggt ttttctttac caatatttgg ttctacgcca aaggtaaaca
    43981 gcaattgtag caacgtgtcg cggtcttctt cggtatcagc aacattgctc aaatccagtt
    44041 tcgctgccac ttcccgcagt tgcgttttgt cggcagagag cgggtcaatt tccagataac
    44101 gcaagaaagc ttgttgataa gaaaggcttt ctgctgccgg gcagtccagc acctgttgta
    44161 agagatcgtc cacctcgttc atcaaccggt acatatcata gtgcggtcga taccactcaa
    44221 gcatagtgaa ctcagggttg tgataacgcc ccatctcttc attacggaaa ctgcggcaca
    44281 gctggaatac cggcccgcaa ccggcaacca gcaggcgttt catatggtat tccgggctgg
    44341 tcattaacca gagattcatc ccctgcgaat gcccggggcc aacgaaacgt gtctcaaacg
    44401 ggaccaaatg aatatcggtt accgtcgcct ggctcataca aggcgtctcc acctccagca
    44461 ctccacgatc ggcaaagaaa cgacggatct ccgccataat cgccgcgcgt tttaataagt
    44521 taggaatgga tgcgctcggc tgccaggatg ccgtttcgct catagttaaa tctccagttt
    44581 ttgacaaggg cacgaagtct actcgcaacg cgacggcgag acaaatttta cgcaggaata
    44641 aaacaacggt gggcagtgac taaaaaaagc acgatctgat ggtttagtaa ttaaattaat
    44701 catcttcagt gataatttag ccctcttgcg cactaaaaaa atcgatctcg tcaaatttca
    44761 gacttatcca tcagactata ctgttgtacc tataaaggag cagtggaata gcgttcgcag
    44821 accgtaactt tcaggcactt accctgaagt acggggctgt gggataaaaa caatctggag
    44881 gaatgtcgtg caaacctttc aagccgatct tgccattgta ggcgccggtg gcgcgggatt
    44941 acgtgctgca attgctgccg cgcaggcaaa tccaaatgca aaaatcgcac taatctcaaa
    45001 agtatacccg atgcgtagcc ataccgttgc tgcagaaggg ggctccgccg ctgtcgcgca
    45061 ggatcatgac agcttcgaat atcactttca cgatacagta gcgggtggcg actggttgtg
    45121 tgagcaggat gtcgtggatt atttcgtcca ccactgccca accgaaatga cccaactgga
    45181 actgtggggg tgcccatgga gccgtcgccc ggatggtagc gtcaacgtac gtcgcttcgg
    45241 cggcatgaaa atcgagcgta cctggttcgc cgccgataag accggcttcc atatgctgca
    45301 cacgctgttc cagacctctc tgcaattccc gcagatccag cgttttgacg aacatttcgt
    45361 gctggatatt ctggttgatg atggtcatgt tcgcggcctg gtagcaatga acatgatgga
    45421 aggcacgctg gtgcagatcc gtgctaacgc ggtcgttatg gctactggcg gtgcgggtcg
    45481 cgtttatcgt tacaacacca acggcggcat cgttaccggt gacggtatgg gtatggcgct
    45541 aagccacggc gttccgctgc gtgacatgga attcgttcag tatcacccaa ccggtctgcc
    45601 aggttccggt atcctgatga ccgaaggttg ccgtggcgaa ggcggtattc tggtcaacaa
    45661 aaatggctac cgttatctgc aagattacgg catgggcccg gaaactccgc tgggcgagcc
    45721 gaaaaacaaa tatatggaac tgggtccacg cgacaaagtc tctcaggcct tctggcacga
    45781 atggcgtaaa ggcaacacca tctccacgcc gcgtggcgat gtggtttatc tcgacctgcg
    45841 tcacctcggc gagaaaaaac tgcatgaacg tctgccgttc atctgcgaac tggcgaaagc
    45901 gtacgttggc gtcgatccgg ttaaagaacc gattccggta cgtccgaccg cacactacac
    45961 catgggcggt atcgaaaccg atcagaactg tgaaacccgc attaaaggtc tgttcgccgt
    46021 gggtgaatgt tcctctgttg ggctgcacgg tgcaaaccgt ctgggctcca actccctggc
    46081 ggaactggtg gtcttcggcc gtctggccgg tgaacaagcg acagagcgtg cagcaactgc
    46141 cggtaatggc aacgaagcgg caattgaagc gcaggcagct ggcgttgaac aacgtctgaa
    46201 agatctggtt aaccaggatg gcggcgaaaa ctgggcgaag atccgcgacg aaatgggcct
    46261 ggctatggaa gaaggttgcg gtatctaccg tacgccggaa ctgatgcaga aaaccatcga
    46321 caagctggct gagctgcagg aacgcttcaa gcgcgtgcgc atcaccgaca cctccagcgt
    46381 gttcaacacc gacctgctct acaccattga actgggccac ggtctgaacg ttgctgaatg
    46441 tatggcgcac tccgcaatgg cacgtaaaga gtcccgcggc gcacaccagc gtctggacga
    46501 aggttgcacc gagcgtgacg acgtcaactt cctcaaacac accctcgcct tccgcgatgc
    46561 tgatggcacg actcgcctgg agtacagcga cgtgaagatt actacgctac cgccagctaa
    46621 acgcgtttac ggtggcgaag cggatgcagc cgataaggcg gaagcagcca ataagaagga
    46681 gaaggcgaat ggctgagatg aaaaacctga aaattgaggt ggtgcgctat aacccggaag
    46741 tcgataccgc accgcatagc gcattctatg aagtgcctta tgacgcaact acctcattac
    46801 tggatgcgct gggctacatc aaagacaacc tggcaccgga cctgagctac cgctggtcct
    46861 gccgtatggc gatttgtggt tcctgcggca tgatggttaa caacgtgcca aaactggcat
    46921 gtaaaacctt cctgcgtgat tacaccgacg gtatgaaggt tgaagcgtta gctaacttcc
    46981 cgattgaacg cgatctggtg gtcgatatga cccacttcat cgaaagtctg gaagcgatca
    47041 aaccgtacat catcggcaac tcccgcaccg cggatcaggg tactaacatc cagaccccgg
    47101 cgcagatggc gaagtatcac cagttctccg gttgcatcaa ctgtggtctg tgctacgccg
    47161 cgtgcccgca gtttggcctg aacccagagt tcatcggtcc ggctgccatt acgctggcgc
    47221 atcgttataa cgaagatagc cgcgaccacg gtaagaagga gcgtatggcg cagttgaaca
    47281 gccagaacgg cgtatggagc tgtactttcg tgggctactg ctccgaagtc tgcccgaaac
    47341 acgtcgatcc ggctgcggcc attcagcagg gcaaagtaga aagttcgaaa gactttctta
    47401 tcgcgaccct gaaaccacgc taaggagtgc aacatgacga ctaaacgtaa accgtatgta
    47461 cggccaatga cgtccacctg gtggaaaaaa ttgccgtttt atcgctttta catgctgcgc
    47521 gaaggcacgg cggttccggc tgtgtggttc agcattgaac tgattttcgg gctgtttgcc
    47581 ctgaaaaatg gcccggaagc ctgggcggga ttcgtcgact ttttacaaaa cccggttatt
    47641 gtgatcatta acctgatcac tctggcggca gctctgctgc acaccaaaac ctggtttgaa
    47701 ctggcaccga aagcggccaa tatcattgta aaagacgaaa aaatgggacc agagccaatt
    47761 atcaaaagtc tctgggcggt aactgtagtt gccaccatcg taatcctgtt tgttgccctg
    47821 tactggtaag gagcctgaga tgattaatcc aaatccaaag cgttctgacg aaccggtatt
    47881 ctggggcctc ttcggggccg gtggtatgtg gagcgcaatt attgcgccgg tgatgatcct
    47941 gctggttggt attctgctgc cgctggggct gtttccgggt gatgcactga gctacgagcg
    48001 cgttctggcg ttcgcgcaga gcttcattgg tcgcgtattc ctgttcctga tgatcgttct
    48061 gccgctgtgg tgtggtttac accgtatgca ccacgcgatg cacgatctga aaatccacgt
    48121 acctgcgggc aaatgggttt tctacggtct ggctgctatc ctgacagttg tcacgctgat
    48181 tggtgtcgtt acaatctaac gcatcgccaa tgtaaatccg gcccgcctat ggcgggccgt
    48241 tttgtatgga aaccagaccc tatgttcaaa acgacgctct gcgccttatt aattaccgcc
    48301 tcttgctcca catttgctgc ccctcaacaa atcaacgata ttgtgcatcg cacaattacc
    48361 ccgcttatag agcaacaaaa gatcccgggt atggcggtgg cggtaattta tcagggtaaa
    48421 ccttattact ttacctgggg ctatgcggac atcgccaaaa agcagcccgt cacacagcaa
    48481 acgttgtttg agttaggttc ggtcagcaaa acatttacgg gcgtgcttgg tggcgacgct
    48541 attgctcgag gggaaatcaa gttaagcgat cccgcaacaa aatactggcc tgaacttacc
    48601 gctaaacagt ggaacgggat cacgctatta catctcgcaa cctacaccgc tggcggcctg
    48661 ccattgcagg tgccggatga ggtgaaatcc tcaagcgact tgctgcgctt ctatcaaaac
    48721 tggcagcctg catgggcgcc aggaacacaa cgtctgtatg ccaactccag tatcggtttg
    48781 ttcggcgcac tggctgtgaa gccgtctggt ttgagttttg agcaggcgat gcaaactcgt
    48841 gtcttccagc cactcaaact caaccatacg tggattaatg taccgcccgc agaagaaaag
    48901 aattacgcct ggggatatcg cgaaggtaag gcagtgcatg tttcgccagg ggcgttagat
    48961 gctgaaactt atggtgtgaa gtcgaccatt gaagatatgg cctgctgggt acgaagcaat
    49021 atgaatcccc gtgatatcaa cgacaaaaca cttcagcaag ggatacaact ggcacaatct
    49081 cgctactggc aaaccggcga tatgtatcag ggcctgggct gggaaatgct ggactggccg
    49141 gtaaatcctg acagcatcat taacggcagt gacaataaaa ttgcactggc agcacgcccc
    49201 gtaaaagcga ttacgcctcc aactcctgca gtacgcgcat catgggtaca taaaacgggg
    49261 gcgaccggcg gatttggtag ctatgtcgcg tttattccag aaaaagagct gggtatcgtg
    49321 atgctggcta acaaaaacta tcccaatcca gcgagagtcg ccgccgcctg gcagattctc
    49381 aacgctctgc agtaaaattt catcgggtcc gaattttcgg accttttctc cgcttttcct
    49441 tgctgtcatc tacacttaga aaaaaaccag taaggaaaca tttatgcgcc tgctccctct
    49501 cgttgccgca gcgacagctg catttctggt cgttgcctgc agttctccta cgccgccgcg
    49561 tggcgtgacc gtagtgaata atttcgacgc caaacgttat ctcggtacct ggtatgagat
    49621 tgcccgtttt gatcaccgct ttgagcgtgg actggaaaaa gtcaccgcaa catacagcct
    49681 gcgtgatgac ggcggcctga atgtcattaa taaaggctat aaccctgaca gaggaatgtg
    49741 gcagcagagt gaagggaaag cgtactttac cggcgcacca actcgcgctg cgctgaaagt
    49801 gtcattcttt ggtcctttct atggcggtta taacgttatt gcactcgatc gggaataccg
    49861 ccatgcgctg gtttgcggcc cggaccgcga ctacctgtgg atactctccc gcacgccaac
    49921 catttctgac gaagtgaaac aggagatgct ggcagtcgcg acccgggaag ggtttgatgt
    49981 cagtaaattt atttgggtac agcagcccgg cagttagtga gtgctgagtt tcagaccaat
    50041 aatccccaat acgattaacg ccagactcgc caggcgcatc gggttagcgg actcaccgag
    50101 cagcacaatg ccggtgatgg ccgcgccgac tgcgccaata cccgtccaca cggcataagc
    50161 cgtccctact ggtaacgatt tcatcgccca ggcaagtagc gccatactga caatcatcgc
    50221 cgtcacggta ataacactcg gcgtcaaacg actaaagccg tgggtatatt tcaggccaac
    50281 ggcccatacc acttccagca gaccagcaat aactaagata atccaggaca tatcaggctc
    50341 cggagaattg gggccgtccc cggtgaaaag acgcgtttgc aggtcgtcct gcaaagctga
    50401 tggttgaacg gatattttgc caggtggcag gggaatttca actattacct ttgtgactat
    50461 cgcagagaaa acaaacggca cgacacagct gtcgtaccgt tattgcttat tgctgcgctt
    50521 tcgttgctgc accagaaatc gcgttaccgc catcagaaat gtcttcacca acgccacgcg
    50581 tggtgttgca ggcagttaat actgttgaaa gcaccagaac agaaaagatc gctgcaattg
    50641 tcttcttcac cataacgtct tccttttagg taatgttatt tatgtttgcc tatagcaaat
    50701 taataataga caacatcgcc aaaaatgatg gaaatctgat gatttttcgg aagagaagaa
    50761 aaattagctg gcagcgcggg agatggagtt gccgagatgt ttgatgtctt cgccgaaacc
    50821 gcgagcggtg ttacagcccg tgagcagcgt gctggcaagc aaaacaagaa cgataaggcg
    50881 tttcatcatc tctgacctga ataagtgatg gtgcagcctg caggccgcac cacaaccgca
    50941 ttacttcacg cgagagacgt attcaccaga gcgggtatcc actttgatga cttcgccgat
    51001 ttgtacgaac agcggaactt taaccacagc gccagtagac agggtagccg gtttgccgcc
    51061 agtacctgcg gtatcacctt tcaggcccgg atcggtatca acgatttcca gttcaacgaa
    51121 gttcggcgga gtaacggaga tcggctgacc attccacaga gttacgatac actctgcctg
    51181 atccagcagc catttcgcgt tgtcgccaat tgcttttgcg tcagcagaca gctgctcgaa
    51241 agtttcgttg ttcatgaagt gccagaactc accgtcgttg tacaggtaag tcaggttcat
    51301 atcgacaaca tcagcgcctt cagcggaatc agtagatttg aaggtttttt ctacgcgagt
    51361 accggtcagc agacgacgca gtttaacgcg agcaaatgcc tggcctttac ccggttttac
    51421 gaattcactc gcttcaaccg cgtaaggttc gccgtctaac atgattttaa gaccagcacg
    51481 aaaatcgttg ctatagtacg ttgccataag gccctctgaa atttgttaat tggtagctaa
    51541 gccacaaaat ggcgcatatt gtaaccctaa ataccccatc cagagaagat tggttaacgc
    51601 aacttgccga tgttgtgacc gatcctgatg aacttctgcg tcttttgaat atagacgcgg
    51661 acgaaaaact gttggccgga cgcagcgcca aaaagctgtt tgccctgcgt gtgccccgct
    51721 catttatcga tcgcatggag aaaggcaatc cggacgatcc gcttttgcgt caggtaatta
    51781 cctcgcaaga tgagtttgcc atcgcgcccg gattctccac cgacccgctg gaagaacagc
    51841 acagcgtagt gcctggtttg ttgcataaat accacaaccg ggcgcttttg ctggtcaaag
    51901 gcggctgcgc ggtaaattgc cgttattgct tccgtcgcca cttcccttat gcagaaaatc
    51961 agggcaacaa gcgtaactgg caaactgcac ttgagtatgt tgctgcgcat ccggaactgg
    52021 acgagatgat tttctccggc ggcgatccgc tgatggcgaa agatcacgag ctggactggt
    52081 tgctcacaca actggaagcc atcccgcata tcaaacgtct gcggattcac agtcgtctgc
    52141 cgattgtgat cccggcacgc atcacagatg cgctggttga acgcttttca cattctacgc
    52201 tgcaaatctt gctggtgaat cacatcaacc atgccaatga gatagatgaa acattccgtc
    52261 aggcgatggc taagttgcgc cgggtaggcg ttactttgct gaaccagagc gttctgttac
    52321 gtgatgtgaa cgataacgca caaacgctgg caaacctgag taatgcgttg ttcgatgccg
    52381 gcgtgatgcc gtattacctg catgtgctcg ataaagtaca gggtgcggcg cattttatgg
    52441 tgagtgatga cgaagcacgg cagattatgc gtgagttgct gacactggtg tcgggatatc
    52501 tggtgccgaa actggcgcga gaaattggcg gcgaacccag caaaacgccg ctggatctcc
    52561 agctacgcca gcagtaactg attaaacagg gaagtcaaac attaaaattg cctgatgcgc
    52621 tacgcttatc aggcctacat cgtgcattga aattgaatgg atttgcaatg aactgtaggc
    52681 cggataaggc gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc
    52741 attaaacagg gaagtttaat ctttgcccgt tgcataacct ttaattattc atatgcattg
    52801 ctgttgttac tctttatata actaacacca ataattaacc tttcttttct gtttatatag
    52861 agtgagtacg gctatttatg ttattttaac aactattact accaagccca tttatctggc
    52921 ttataaatta aaattaatta tttaattgag aaatggttag ggagaaccta catggccata
    52981 agcatcaagg gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg
    53041 ctgaaaatta aagaacctcg caataaagaa tcgttgttct ttatgtctgt catggaactg
    53101 cgcgacctgc tgattgcgct ggaaagtcgt ctgcatcaaa agcataaact ggatgctgct
    53161 gcacgccttc agtatgagca agctcgcgat aaagtcatta agaaaatggc agaaaatatc
    53221 ccagaaattc tggttgatga gcttaaaaat gccgatatta atcgtcgggt taacacgctg
    53281 gaacttaccg ataatcaagg cgaaaatctt accttcgtat taacgctaca tgacggtagc
    53341 aaatgtgagt tagtcgtcaa tgagttgcag attgaaatgc tggcgcgagc aatcattcat
    53401 gcaattaata atgcggaaat gcgtgaactg gcattacgta ttacctcact gttagatttc
    53461 ctgccgttat acgacgttga ctgccagcaa aatggcaatc tggaatatga cacctattcc
    53521 caaccggaat ggaaacataa cttgttcgac cattatctgg cggtgcttta ccgctttaaa
    53581 gatgaaagtg gcaaagaaca atttagcggc gcagtggtga aaacgcgtga agcaacgccg
    53641 ggtaaagaaa tagaggcgat cactcgccga atgcttgatt tcagcccacg actgaaaaaa
    53701 ctcgcgggtg tgccatgtca ggtctatgtt cgcaccgtag cagcaaataa cgcccaaccg
    53761 ctaacccagg atcaatgctt acgcgcgctg catcacttac gtgttcaatc caccagcaaa
    53821 actgcaccac aggcgaaata atctcatctt tgctaatgcg aggctggagc catccagcct
    53881 gaaatgactg ataacgtgat cgttggtcat gccggatgcg gcgtaaacgc cttatccggc
    53941 ctacaaattc tcaagcattc aatcagttgc aatgcattat gtaggcctga taagcgaagc
    54001 gcatctggca attatacgtt tgtcaccatc acatcagttc gggcacttat atacctggcc
    54061 gataatctgg ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctactgat
    54121 gccataaatc acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc
    54181 gcgcatagaa ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt
    54241 cgcagtacca atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg
    54301 accggcagca ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac
    54361 aatccctgct aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgtcac
    54421 tcgcgtcgtc cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac
    54481 atttctgccc gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc
    54541 atgccaccca tgccgcccat accgccagca gcgcctaagt cagctgcatc gtttttcggc
    54601 aggtcggtaa ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc
    54661 agagcagaac gggttacttt ggttggatcc aggataccca tgtcgatcat gttgccgtat
    54721 tcttcggttg ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca
    54781 acagacggtt cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc
    54841 agtgcaactt tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta
    54901 gacgctacgc ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca
    54961 cgggtcgcgt gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta
    55021 gcagcaccta ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ctcctgcagt
    55081 ttttcacggt cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg
    55141 ccctggattg cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc
    55201 acaacacgtt tagcctgacc caggtcttcc agggttgctt tttccagctc cataccgatc
    55261 tcttcagaga ttacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga
    55321 cgatcgccga agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta
    55381 acaaccagag ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg
    55441 cctgctttgg caacggcttc caaaaccggc agcatttcgc ggatgttgga gattttcttg
    55501 tcagccagca ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg
    55561 aagtaaggag acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc
    55621 tgcagaccgg taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct
    55681 tcagcgatca gtttacctac ggtttcgtcg gagttagcgg agatagtacc aacctgagca
    55741 atcgctttag agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta
    55801 acagctttgt cgataccacg tttcaggtcc atcgggttca tgcccgcggc aacagctttc
    55861 agaccttcag tgatgatagc ctgagccagt acggttgcag tggtggtacc gtcgcctgca
    55921 gcgtcgttcg ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg
    55981 tcttccagtt cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg
    56041 aaagatttat ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct
    56101 gccagtacgt ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct
    56161 ttagctgcca ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc
    56221 ttcaacaatt gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt
    56281 ctcggatttc acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag
    56341 cggcttcact tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg
    56401 ggtggattta gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac
    56461 ttctttacgc ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc
    56521 tttgagaaag tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt
    56581 gaccagagaa atggggatga ggcttcgccc cttcaagggg gaaaaagaaa aaaaattctg
    56641 atcagcacaa aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat
    56701 cgccgtcata agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt
    56761 tgggcgcaat cggacatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg
    56821 gcattggcct gctatcgacg tcattattag gcactggcgt gtttgccgtt cctgcgttag
    56881 ctgcactggt agcgggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt
    56941 tcccgattgc gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggcgtcg
    57001 cgcacttcgt cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggctgtttt
    57061 tatcggtcat tcccgtgggt ttgcctgccg cgctacaaat tgctgccgga ttcggccagg
    57121 caatgtttgg ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgttgg
    57181 tgtggtatat cggtactcgc ggtgccagtt ccagtgcaaa tctacaaacc gttattgccg
    57241 gacttatcgt cgcactgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgagta
    57301 tccccttccc cgcgccagga aatatcgaac ttaccgggtt atttgctgcg ttatcagtga
    57361 tgttctggtg ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc
    57421 cagaacgtga ttttcctcgt gctttgatga ttggtctgct gctggcagga ttagtctact
    57481 ggggctgtac ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag
    57541 catcgcttcc aaaaattgta gtacaactgt tcggtgtagg agcgttatgg attgcctgcg
    57601 taattggcta tctggcctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc
    57661 tggtctggtc gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc
    57721 atatcccgaa taatgccctc aatgcggtgc tcggctgctg cgtggtgagc acgttggtga
    57781 ttcatgcttt agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta
    57841 tgatttatct gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac
    57901 tggcggtggt tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc
    57961 tctatgcgct gatcatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg
    58021 aaaatggcat aaccacataa tccggcgttt cgacattaat cctggcgatc gtctttatga
    58081 tcaaggcggt cgcgctcatc atcctttcgc tggtactcac catcaaaagt attaccgcca
    58141 ccggtcccgg cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac
    58201 ttcactgtca gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg
    58261 aaaaagcccg gtaataaaag cagcaaaccg gcaatgatca gcgaaacact tttaatcatc
    58321 tccgccgctg ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag
    58381 ccctggttac gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc
    58441 aacaataccc ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa
    58501 aggaaaatgg caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta
    58561 aacccggcta cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa
    58621 atttcaagat gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac
    58681 aaattaataa ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc
    58741 ctcaagcagc atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa
    58801 acattcgaaa ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga
    58861 aggttcacat gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc
    58921 cagctgatgc ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca
    58981 acaacaaaat cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg
    59041 cagctatggc aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg
    59101 ccgcatgtga tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct
    59161 accagggcgg cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg
    59221 gtctggaact gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg
    59281 ttaacaaatg tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact
    59341 cttccctgat taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag
    59401 ctgtcgaatt ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga
    59461 tgaccctcgg tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca
    59521 tccaacgtac cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg
    59581 gtctgaacac gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagttactg
    59641 gcttcccatg cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg
    59701 ttatggttca cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc
    59761 tgcgcttgct ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc
    59821 aggcgggctc ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc
    59881 aggtatgctt caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc
    59941 agctgcagtt gaacgttatg gagccggtca ttggccaggc catgttcgaa tccgttcaca
    60001 ttctgaccaa cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca
    60061 aagaagtgtg cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt
    60121 tcatcggtca ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg
    60181 tacgtgaagt cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct
    60241 ccgtacagaa tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac
    60301 agtaatcgta cagggtagta caaataaaaa aggcacgtca gatgacgtgc cttttttctt
    60361 gtgagcagta acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta
    60421 atatgctagt tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg
    60481 ggggaatagg tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg
    60541 ttaaacccgg taacatccca ttcgatgtca tttccattat catggcggtt atcgccgcta
    60601 tttctgccat gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc
    60661 tgcgccgtaa cccgaaatac atcacgatcc tcgcaccgat cgtgacctat ttcctgacta
    60721 tctttgctgg tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga
    60781 aggaacaagg cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg
    60841 cgatcaccgc atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc
    60901 atggcatcag ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc
    60961 tggtgatgtc cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt
    61021 atcgcaagcg tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca
    61081 aatccggtgc aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct
    61141 atgcaatcat caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca
    61201 acgcaatcct gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg
    61261 ataccgacaa catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta
    61321 tcctgggtgt tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag
    61381 ataccgctgg tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg
    61441 cttctgctct gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg
    61501 cactgaacgt ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca
    61561 ttctgccgac ctacccgacg ctggttgctg cggtacagat ggatgacacg ggtactaccc
    61621 gtatcggtaa attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc
    61681 tggccgtttg cttcggcttc gtgctgggta gcttcatgct gtaatgacca atcgcggggc
    61741 gttcacgccc cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga
    61801 cctctctctt gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg
    61861 aataccgcgt ctgtcgtggt gctatgtacg gcaccagatg aagcgacagc ccaggattta
    61921 gccgccaaag tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc
    61981 tctctctatt actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa
    62041 actaccgtat ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa
    62101 accccggaac ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc
    62161 aacgcatctt tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg
    62221 acgcgccggg acgttcacaa tttgtccccg cggatcaagc ctttgctttt gattttcagc
    62281 aaaaccaaca tgaccttaat ctgacctggc agatcaaaga cggttactac ctctaccgta
    62341 aacagatccg cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg
    62401 tctggcatga agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc
    62461 ccgtaaccat aaaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg
    62521 ctgatgccgg tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg
    62581 ccaacaacgc agcgtcacag cctgtgtctg tttcgcagca agagcagcac accgcgcaat
    62641 tgcccttttc cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg
    62701 tgctgccaat gtacccactg atttctggca tcgtgctggg cggtaaacag cggctctcca
    62761 ctgccagagc attgttgctg acctttattt atgtgcaggg gatggcgctg acctacacgg
    62821 cgctgggtct ggtggttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat
    62881 acgtgctcat tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt
    62941 ttactctaca actcccctcc tcgctgcaaa cgcgcctcac gctgatgagc aatcgccaac
    63001 agggcggctc acccggcgga gtgtttatta tgggggcgat tgccggactg atctgttcac
    63061 catgcaccac cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt
    63121 ggctgggcgg cggcacgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa
    63181 ttaccgtctt tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa
    63241 ccgcgtttgg ttttgtgatc ctcgcactgc cggtcttcct gctggagcga gtgattggtg
    63301 atatatgggg attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta
    63361 tcaccagcct acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag
    63421 cggcattggt tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc
    63481 aaactcagac gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc
    63541 tcgttgaagc caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct
    63601 gtaaagagtt tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca
    63661 cggtcttact tcaggccaac gtcacggcca acgacgcaca agatgtggcg ctgttaaagc
    63721 atcttaatgt ccttggccta ccgacaattc tcttttttga cggacaaggc caggagcatc
    63781 cacaagcacg cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc
    63841 gccaaccgtg aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag
    63901 atgtactggg agaagccctg aaattattag aattacaagg gattgccaac accacgctgg
    63961 agatggttgc tgaacgtgtg gattatccac tggacgagct gcgccgcttc tggccagaca
    64021 aagaggcaat cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc
    64081 gtcagctgat gctggacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg
    64141 cgttatcgga gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta
    64201 cgttttatcc cgatcctggc caccctattc atcaactggc cgatcagcaa aaaagcgcag
    64261 cctacgattt cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag
    64321 caaagcagat ggaactggtg ctggaaggct gtttaagccg aatgctagtg aatcgtagcc
    64381 aggcggatgt cgacactgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc
    64441 gccagggtgg cgcattgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta
    64501 agcagttgaa tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg
    64561 cgccccgttg cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcggt
    64621 ggttcgattc cgcctccggg caccatatac cagaacacgc tggttaaggg atgagatcct
    64681 tgacgggcgg ttaatacagg atgcggcatc aaagggtctc cgtttgtgta agttgtggtt
    64741 acactgattg agttcggtat cgtatcctca aacagggtgt cttttgcaga gacgccctgt
    64801 tctcgttaac actgtatgct tacattgctg attcagcctg tcaagattta ttcccccctt
    64861 tgcaaatctt gctcactgtg cgctcatctg tatacaaact cagggcatct tccagtgtct
    64921 gcgctgcaga ctccatacag acatcaacat accccatggt cgttgtaaga ctcctgtgtc
    64981 ccagtatgga tttgaccaga tgtaaattac gttcaggata tttcatcagt ctggttgcca
    65041 gcgtatgtct gaaacgatgt ggtgaaacag gaaaaccaca ttctcgcgaa agtcgccgga
    65101 aaaatgaacg tacggtctga tgcgcccgaa cgggacagta ctgatatctg tcggcagaga
    65161 tcggctcact gaaacgacag acgtcaaaaa gatagtcatc atcacctgcc ccgcactgac
    65221 gggcccgctc cagcagtttt tccatcgata ccttcagggg agccaccaga ggaacgcccc
    65281 attccctgtg agttttactt ccctccagct taagatcaat tatgctttca tccggacaga
    65341 catccctcac acggatatgc ttcagttgat taaagcgcat tccggtataa cgcagaacat
    65401 ccagaactgt cagccagtac caggcagggt atatagcatt gcgtcgacga ctattcacca
    65461 tgttctgctc ttccgcttct gcaaacctct gcacaaccag gtaaaccctg agcatctgat
    65521 tgtcactcag tgtctttttc tttttttcag tactcgcaac tgatgcttcc accaacgggt
    65581 tttctgtctg aggcagcagt ttcatcttga tcccgaatcc cataattgcc cgcaagtggg
    65641 ccaccttgtt attccaggtc acctccctgc gcttctgctc tttcaggaca taacgccggt
    65701 atcgctgaac atcacggaca gtcacctccc ccggcaaaat atcaccgata aattccctga
    65761 ataccctgac aactttgcag tagctccact ctgtatccgg ccgaagattc tttgaaaaaa
    65821 agtactcttc cagcagttgt tcccaggtga tttttgtgac ggaatcgtca ttctgcatta
    65881 tatattcccc ttgtttactg tgctcccgca ctctccggtc aaattctgca catcatcgcc
    65941 tttactcacc cgaaaacaat aagcgggctg tctccgggaa ccgtaatatt cccgtacaga
    66001 aacctgccct taaccagata tcccgaaacc attttgtaac gtccggaatg ttctgcgtca
    66061 ctataaagac gggcctgaaa aaaacgctct cctgaacgta ccttatggat acctgcccgt
    66121 tcaaagagcg actgaacata acgacgatcc tgcccggaac cactctcctt cagaaaacga
    66181 taaaaaatcc ccggggcaca cagaaaaaca tatccggaaa caacatgaac atttgaatca
    66241 acgctgttaa ccgaaagtga accctccctc agcccctctt ttaaccatct gataaattca
    66301 ctctctaccc tgctatcctc tgtcagggaa aactctgccg ttttttccac tgtttccggg
    66361 atacggtttc cctgattttc tgttacacat ccgctctctt ccggtaactc aataccgctt
    66421 tctttatccg gcggcacgcc tgacgctatc tgccctccct catccgacaa atcagcagcc
    66481 gaaaacagcg taaggagcat tccggtatca gcatcagttt cgttcccctc atccacctcc
    66541 tgttccgctg tccggccctc attttcggca tcctccactg gcgactgtac aaccggcaac
    66601 ggctgaatga cgctttctgc tgaaattgct gctgactgca gcagtaccgg aacaacctca
    66661 gccaccggaa cttcggccac atctcctgca gcgaggggac tcacgacttc ctccatagct
    66721 tcctgtaata ctgtacgtat cagtgccatt tcaggatgct gatgccagac tgctccggca
    66781 agattctgta atgcccccgg gacagtggca agccaggctc ccgcctctcc gggcagcagt
    66841 tgtcccgcca tcagggctgc cagttgccgt ccctccgttc cctggcaggg catcttcctg
    66901 aaccggaacc ggtatacgcc ctcaggtaca gatatccccg gctgccagca tctgccgtcg
    66961 gcctgttcgc cttccagttc cgccagcaac ggcaggtgat aaaacagagc cgaccagaac
    67021 accaccatgt tccacacact cccctgcgcg gcctgctctt ctgctgtcgc tcccggtgga
    67081 aacattctgc ctctggccag ccggacagca tacgtcgtga actgcagggt caggtcacca
    67141 aaaccaccgg tgtaacccca gcgaccttct gctgtggccg gcagattctg actgacagac
    67201 aacaactgat gcacaggatc aagacaaagg cgctgataca tatccaccgg catggaactg
    67261 ttttcccata actgtttcag acaccttttc ctgaacggtg tggataccag tacatcagcc
    67321 ggcaatggtg taaaataccc ctccggcacc gtttccccgg gaatatccgt ccggacatca
    67381 gaacattctg tcttcacagg aaataacctt ttcaggcgtt caagcatgcc cattctcctg
    67441 aacccgacct gatgtgtcgt acccggtccc ccgcagacct tcagttttct gctgttcttc
    67501 tgccagttgg tgcatcaggt cattcagtac cagcgtcctg agagcccggg tgacagccac
    67561 atataacaga caggtttcat catgacgttc ggcctccgtc atcagcggat ccgtgatatc
    67621 ctggaaatcc tcatccagca ttacaaccgg ccactccagc cccttgctcc ggtgtgccgt
    67681 tgacaccgtg atatctgcat cctgctcatt cgttaccgta tactgccgga gaagttccag
    67741 ttttttcggt aacggaaaac acaactccag aagccgaagg gactgattca tctccacatc
    67801 cttcgtggat tttgcaatat atttatattc ttcaaaattc ctgtagtccc ggcgcagcct
    67861 gtctgaatgc atccgttccg gcatatcaac ctggaaccag tagagatcct caagttcccc
    67921 ggtcctgtaa ccttcgattc cccccaccca gtaaaccttt tgccctttca ttgccgccat
    67981 cagcgcagta ccaatcacac ccgcgacagt acgactgagt accgtgtaat gcccctgcag
    68041 atgttcacgc gcatggtatt tcagcaatac ctcatcatca ccaccacaac ctttcacctc
    68101 tcgtgtttca ccttcacgct gcagaagcag atttgccata cgagccaccg ccggaccaaa
    68161 acggaaactc tgtgtcagcc acaaccgatc cgcattcttc agtgcaggat gggaaagcgc
    68221 attattcgca ccccgaaagc ggtaaatctg ttgataacaa tctccaacaa ggaccacccg
    68281 acacttctgc cccagaacca gagcagtggt caccggattc gcatcctgcg cctcatcaaa
    68341 caggatggtg tcccatcggt gagacaggtc tgcaccggac aactggaaga gtttcagata
    68401 agtatcgtgc gtcaccggga acgtcccccc ggggttgctc atttcgttcc agagcaactg
    68461 caccgcccga agcgcatctg ctggcggaac accccgcatc tcatccttat ccggcaggtg
    68521 atgcaggccc ggtaccgcat cggcagaaca gataaagtga ttcagccccg tccctgccac
    68581 ccgtgccaga gcccagtatc gtgtattcag ctttctcgcc acatcagtaa tacgtaatcc
    68641 cggaaccaga cgatgacggt agtgtcttcc aacccgggca tacgccagct gatgtgacgt
    68701 tctgcattcc acgttaaagg gaaatttacg ctctgcctca tcacgtattg cccggttata
    68761 ggcaagatag agcatacggc tgtccggact ggcccgggca aactgcacca gcgtactcgt
    68821 cttccctgtg ccggaaaacg cattcacaac cagttgctgc ccctgccagt taataatggc
    68881 gtactgttca tctgtgggat taaaagacat gtattctctt ctccggaaaa acatttccgt
    68941 aatctgatgc atcaggggta tgcccaccac aaataactct gtacagcaac agaaaaattt
    69001 ccctggtcat atgatgaatt aatcatcgcc atcacaccgg atgcttctca tcctgtcctc
    69061 agaaaggcag gtggtctcct gtctgccgcg cttatccagg ataccgggac ctgaaaggtc
    69121 ataatctggc aggaaaaaat aatgtccgaa gtaaaaaaac acgtttctta ttcattaaca
    69181 gttgcagcta tgctgtcagc cgccatcgtc tgtcagacaa cacatgctga tatcaacaca
    69241 ccgaacatca ccggagaaaa aaacaataca acaacacgat tctccggaaa tatttacggc
    69301 agtgaaaata ctgtcacatc agagtcaaac agtctggttg tgggtaacag taattccgtg
    69361 gagggagggt ataacaacat tgttattggt aattcctcca cggtcaaagc ttccggtttg
    69421 atcagaaaag tgggcgaaga tattgtgctg gatggttaca gcgtcagcct cggtaatgcc
    69481 accagaatta acggtgattc atctgttgcc ataggtctga catcgtccgt aaccgggaac
    69541 gactccgtgg cactgggcag tcattccagt gctaacggaa acaatatcgt atccgtcggc
    69601 tctgacacac tgaaacgccg catcacaaat ctggcacagg ggaaagatga ccatgatgcg
    69661 gtaaatctga tgcagatgac cagtgctgtt aaccgggaag ctggagaaag aataaaatca
    69721 acaaacaacc tgcagaatca gcttaatacc ctctctggtc aggtcataac tgaaaccaga
    69781 gcccgaacag aaggtgatgc aacagcgctg gctgcagcca gagcccacag cgatgaaaac
//
LOCUS       DAZFKF010000015        69822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFKF010000015 DAZFKF010000000
VERSION     DAZFKF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.015"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(26..250)
                     /gene="cspD"
                     /locus_tag="ACHKA1_001201"
     CDS             complement(26..250)
                     /gene="cspD"
                     /locus_tag="ACHKA1_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO5678885.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            400..474
                     /gene="yljB"
                     /locus_tag="ACHKA1_001202"
     CDS             400..474
                     /gene="yljB"
                     /locus_tag="ACHKA1_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO5678886.1"
                     /translation="MNQKVEAVNAIDRNVTDVADANDR"
     gene            573..893
                     /gene="clpS"
                     /locus_tag="ACHKA1_001203"
     CDS             573..893
                     /gene="clpS"
                     /locus_tag="ACHKA1_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO5678887.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            924..3200
                     /gene="clpA"
                     /locus_tag="ACHKA1_001204"
     CDS             924..3200
                     /gene="clpA"
                     /locus_tag="ACHKA1_001204"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO5678888.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(3605..3692)
                     /locus_tag="ACHKA1_001205"
     tRNA            complement(3605..3692)
                     /locus_tag="ACHKA1_001205"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3656..3658),aa:Ser,seq:gga)
     gene            complement(3946..4164)
                     /gene="infA"
                     /locus_tag="ACHKA1_001206"
     CDS             complement(3946..4164)
                     /gene="infA"
                     /locus_tag="ACHKA1_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO5678889.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(4449..5153)
                     /gene="aat"
                     /locus_tag="ACHKA1_001207"
     CDS             complement(4449..5153)
                     /gene="aat"
                     /locus_tag="ACHKA1_001207"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO5678890.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(5195..6916)
                     /gene="cydC"
                     /locus_tag="ACHKA1_001208"
     CDS             complement(5195..6916)
                     /gene="cydC"
                     /locus_tag="ACHKA1_001208"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO5678891.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNTGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAVIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(6917..8683)
                     /gene="cydD"
                     /locus_tag="ACHKA1_001209"
     CDS             complement(6917..8683)
                     /gene="cydD"
                     /locus_tag="ACHKA1_001209"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO5678892.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(8806..9771)
                     /gene="trxB"
                     /locus_tag="ACHKA1_001210"
     CDS             complement(8806..9771)
                     /gene="trxB"
                     /locus_tag="ACHKA1_001210"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO5678893.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            10316..10810
                     /gene="lrp"
                     /locus_tag="ACHKA1_001211"
     CDS             10316..10810
                     /gene="lrp"
                     /locus_tag="ACHKA1_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO5678894.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            10945..15051
                     /gene="ftsK"
                     /locus_tag="ACHKA1_001212"
     CDS             10945..15051
                     /gene="ftsK"
                     /locus_tag="ACHKA1_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO5678895.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     DITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            15206..15817
                     /gene="lolA"
                     /locus_tag="ACHKA1_001213"
     CDS             15206..15817
                     /gene="lolA"
                     /locus_tag="ACHKA1_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO5678896.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            15828..17171
                     /gene="rarA"
                     /locus_tag="ACHKA1_001214"
                     /gene_synonym="mgsA"
     CDS             15828..17171
                     /gene="rarA"
                     /locus_tag="ACHKA1_001214"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO5678897.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIILPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            17262..18554
                     /gene="serS"
                     /locus_tag="ACHKA1_001215"
     CDS             17262..18554
                     /gene="serS"
                     /locus_tag="ACHKA1_001215"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO5678898.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(18794..18934)
                     /locus_tag="ACHKA1_001216"
     CDS             complement(18794..18934)
                     /locus_tag="ACHKA1_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5678899.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMIHRSPCEFRLNIMWSEFAAFLQC
                     KP"
     gene            complement(19065..19367)
                     /locus_tag="ACHKA1_001217"
     CDS             complement(19065..19367)
                     /locus_tag="ACHKA1_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO5678900.1"
                     /translation="MFHCPFCGASARIRTSKAANDTGTIRYKYYQCNNLECSATFSTM
                     ESVHRIIGQPQLIFHDKDIPRETMRKSHQGRSQLTLQLEDCGMTLKPVHYARVPFG"
     gene            complement(19411..20535)
                     /locus_tag="ACHKA1_001218"
     CDS             complement(19411..20535)
                     /locus_tag="ACHKA1_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO5678901.1"
                     /translation="MVLLNGNTVYSPLKELVKMPDFSVSLDREQDKNTLQELNNRVIS
                     LSMTDNRGFEADRVTLVLDDADGQLALPGRGTHIRVMLGWKGEPLVDKGTYIVDEVAH
                     EGPPDRLTLSANSADFREEFNVKREVSWHDVTVERVVSAIAHRYGLKAQISDMLMDIE
                     IDHADQTEESDISFLTRMADMLGAITTVKNGCLLFILPGGGVTADGRPLPSFALARSD
                     GDSHQFRIADRQAYTGVKAYWLDLNHGKKKAVNIKKRKTHAEKSSSREGSYMEGAEGN
                     VFVLRKTYPNEIAAQRAAAARWQQLQRGAAEFSITLARGRAELYPEMHGTVTGFKTEI
                     DNQDWLIAKVEHNIDGSGFTTRLELEARIPEWIAEKESNG"
     gene            20690..21877
                     /locus_tag="ACHKA1_001219"
     CDS             20690..21877
                     /locus_tag="ACHKA1_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO5678902.1"
                     /translation="MAETRFHGVRVRENADLVTAINDIDSSVIGIVAVADDADAKTFP
                     LDKPVLLTRVMDVLGKAGKTGTLYKSLKAIADQVSTRVVVVRVAAAKEGDGQPTQTQL
                     VIGEVKNDGTYTGMQALLVAEQNEAVGVRPRILAVPSLDTKEVVAALVTVAKKLRAFV
                     YAGCPGCNTVQEAVTWRADLAHREVMLIWPDFIAYNPNSGQNEAFPASAYACGLRAKI
                     DHEQGWHKSLSNVAVNNVLGISVPVFWSLQAEDSDAETLNDKEITTLIKRNGFRFWGN
                     RTLDTTTYTFETYTRTAQSLADTIAESQFETVDKPLTPSNVKDVLSDIRAKLSALVTA
                     GKLIGAECWYDVVDNGTTDLRQGVVRIRYKYTPVPPLEDLELHQTFTDEFFAGAFASL
                     GGE"
     gene            21877..22386
                     /locus_tag="ACHKA1_001220"
     CDS             21877..22386
                     /locus_tag="ACHKA1_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO5678903.1"
                     /translation="MAVPNKLRVFTVYIDGENKLGRVTSFTPPKLTRKTESFRGAGMP
                     GSVSVDFGLDDGALDLSFAVGGADAHLFSRYAGSLDEVRLRFSGEYYSDADIRYVDIE
                     VRGRITEIDMGEAKQGEDTEHSYSMKNTWYRLSVDDRETIEFDVLNFIWRIDGKDVLP
                     DRTRSMLGL"
     gene            22434..22823
                     /locus_tag="ACHKA1_001221"
     CDS             22434..22823
                     /locus_tag="ACHKA1_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678904.1"
                     /translation="MDKETEMATDTAVSNGEASAVAVAATAAPKNVVKLTVPVVRGDV
                     SYSEIKIGPLANQAGSLRGLSLLALYNMNCDAMVTWLARVTEPKLKEAEMYAMSVVDF
                     TALSTAAVNFFAPSGSNAPAPENTAEP"
     gene            22844..22981
                     /locus_tag="ACHKA1_001222"
     CDS             22844..22981
                     /locus_tag="ACHKA1_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO5678905.1"
                     /translation="MEDPVADIAVIFNWPPAEIFAMTPAEVVAWRERAAARSGSHNHD
                     E"
     gene            22971..25763
                     /locus_tag="ACHKA1_001223"
     CDS             22971..25763
                     /locus_tag="ACHKA1_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5678906.1"
                     /translation="MMNNLHIRVALAAADTLTQPLRRARETVGALSDSLRETQDSIRS
                     LERQSEAFAAAQTKFDEATRKIAAVQQQLNRLQQAEREGTVLSERQREMMASLTTRLE
                     RLNRVRDVHQRRLEDSRRELERHGIAAEGSSQTIQAAIRRTAEYNEALARQRQALAQV
                     TRAQQRYEQAQERLGRIRNAGAAALAAGAAGLYAGGRAVAPVMDLQRHGAVIAAQAGE
                     GASDAVGYTGVINHMVHTGITPDAQLAAEAVGAVRSTLGGLGAVSENELTRITRKMLD
                     MQAVMGGDIPQHIQAAAIMIKNGLARSSDEAVDLMVAGMQRMGPVMRDELPEILHEYS
                     THFRNMGLTGSETMTLLADMAQQGKFALDKTGDAIKEFSIRGSDMSKASAEAYALIGL
                     DAARMSSAVASGGTAARAALQKTASGLLQIVDPAERANAAIALFGTPVEDLSVDQIPT
                     FLGALARVSDRMADVSGVADRMGQTLRDNLSGDVARLTGTFSALRSGIIGEITDDLRG
                     LVRMATAWGERLQAWVNAHPELVRMIALTTGAVLALTTAMGALSVAAWMVVAPLLKLR
                     LGLALLRAIPAGLSVAGTLFGGLTGVISAVGAALGALTWPVVVAVGALVGGALLVWRY
                     WDRVRAFVSGFIEGVQSALAPFMATASRFAPLLDLVSGALSRILGWFKSLLTPVSSTR
                     DLLEQCTSAGQMFGSTLAGALATVFSPLKALIDGLAWVLEKLGVLPGEAERVKRKLDE
                     ARKTPVAWEWDPEQKKMVQKAWHWSPPGGASVPSGASGTQPPPVALPAGIAPDGNRNV
                     LRHLKGIEGNTGGLLAENRKRIGPGDIVFKHLPRALAVRGQWHEPVVAPSSGGMRALP
                     QEEIQVQQWPTARPAPRMHQQETFASAFQGEIHVHLHGVSSHNPRELARMVGDAVRAE
                     MNRMARAGTGSFRDRD"
     gene            25774..26265
                     /locus_tag="ACHKA1_001224"
     CDS             25774..26265
                     /locus_tag="ACHKA1_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5678907.1"
                     /translation="MMMIYGMFVFQLSTLPHQQVEQSKRWRHVSNERVNRSSSWQYIG
                     SGEDHITMSGVLCPEITGGEVSLTALNTLAYTGLPWPLIDGVGQIYGMYVITEIRTTR
                     QELDRYGMARKIEFTVSFQRCNEDLRERLQSGSVGDLMAGLKNSASSALSSASSALSG
                     LML"
     gene            complement(26276..26863)
                     /locus_tag="ACHKA1_001225"
     CDS             complement(26276..26863)
                     /locus_tag="ACHKA1_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO5678908.1"
                     /translation="MHTIKSARNAAYNRDGTIDCEIQFEGEESWLPYTATEYDNSPHG
                     KKLWGELKAGNHGPVSEYQENDTDRENARRLKNDEINTWRNAMEAASYTFEHNGRKWD
                     YGKSTQARLEPSVAAAKAGILPLDFFWTDAQNNDVPVSADELIALSDAAAKALFAKGM
                     EIHIRQRNMKKEIAALNDTAAILDYKVGWGKEAEA"
     gene            complement(26875..27306)
                     /locus_tag="ACHKA1_001226"
                     /pseudo
     CDS             complement(26875..27306)
                     /locus_tag="ACHKA1_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(27613..28215)
                     /locus_tag="ACHKA1_001227"
                     /pseudo
     CDS             complement(27613..28215)
                     /locus_tag="ACHKA1_001227"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(28215..28838)
                     /locus_tag="ACHKA1_001228"
     CDS             complement(28215..28838)
                     /locus_tag="ACHKA1_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO5678909.1"
                     /translation="MNNRLLPPSSPSFLHHSEQGAQRLSELPIHINTLWNPDTCPAHL
                     LPWLAWALSVDRWDETWSEEARRQIIRDSWHIHRHKGTISAMRRAVEPFGAILSITEW
                     WENNDPPGSFRLDIDISERGLTEETRQEMERMITTVRPVSRHLAAMKMTYVARGVSRI
                     TGATLTGETIVVYPAAEVTSPLSVSGDIHPGGVTYEGITVTIRPDKE"
     gene            complement(28831..29727)
                     /locus_tag="ACHKA1_001229"
     CDS             complement(28831..29727)
                     /locus_tag="ACHKA1_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO5678910.1"
                     /translation="MPTIDLSQLPRPQIIESLDYESILKDVKAFMIDAFPENMRSAVS
                     TAIALESEPLNIIAQAIAFREMLLRQRINDAAAGCMLSHAVSTDLDNLAAIVNAKRLE
                     LKPATDTEAAIMESDSAFRLRAQSGFEGFSVAGPSAAYEYFARSASGLVADARATSPS
                     PATVIVSILSTEGDGTASPELIATVSRALNDETVRPLADRLTVQSATIVSYDIEAVLY
                     FYPGPESEPILAAARDALDTWLKTQNAIGRDVARSAIMATLHVPGVQRVELKKPAQNV
                     VISNTQSARCTSRNISAGGTDE"
     gene            complement(29714..30079)
                     /locus_tag="ACHKA1_001230"
     CDS             complement(29714..30079)
                     /locus_tag="ACHKA1_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO5678911.1"
                     /translation="MTPVCTGMNPHGSGCISDTEHLRHSVNNILTTPRGSRIMRREYG
                     SLLPELLDAPQNDVTRLQCMSAVVMALAQWEPRIAINRVDITWLPDGRALLAISGVLT
                     RTMQPVQMSIPLRGSNADN"
     gene            complement(30076..30657)
                     /locus_tag="ACHKA1_001231"
     CDS             complement(30076..30657)
                     /locus_tag="ACHKA1_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009486481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO5678912.1"
                     /translation="MNAEIMRLLENMVRTGVVSEVDLKNWRIRVTSGGLTTDWLRWNC
                     TRAGAFKIWLPPSPGEQVLLLCPGGSPEMAVVAGSLYSEEHPAPGTKQNEVILLAPDG
                     AEIRYDAEKGALTVKGIKTAEISAETRITLDTPAVECTKHLKARTFELTDGGTLKGDI
                     THSNGNLRSNGVTVHTHVHNGVQSGGSMTGGPK"
     gene            complement(30654..31298)
                     /locus_tag="ACHKA1_001232"
     CDS             complement(30654..31298)
                     /locus_tag="ACHKA1_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO5678913.1"
                     /translation="MMAPDDLHRLDGLFQEILSGLSAAGINKTARAVGQAVRAAQQKR
                     IRSQQSPDGNRWPARKKRVFRVQAGVVFIWNGTQVRRLKNWRTTTGRYGPMITGYDTE
                     RRGIRSFHKADIEEYLEIDTRRRTQSAIKRPPMFEQLRTNRFLKVKTDPGGVSVGFEG
                     RAARIARVHQFGEVSLVSAGNAVRYPQRELLGFSEADRQKVTEIIINNLWRNAR"
     gene            complement(31282..31749)
                     /locus_tag="ACHKA1_001233"
     CDS             complement(31282..31749)
                     /locus_tag="ACHKA1_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5678914.1"
                     /translation="MLKINTLRDAMTDACPWCRANPEKFTVYVNNGGIETTGESPSYL
                     YRYTLGIFVMDFTQDLDELMVPLMRWLYRNQPDLLLNPEKNRDIKFSVAINDDDSTDI
                     LLEIPVWERVIIRRNDDGTVHAEHIGEPPVSRALEAWRVTLEDVTWGQNDGSG"
     gene            complement(31765..31938)
                     /locus_tag="ACHKA1_001234"
     CDS             complement(31765..31938)
                     /locus_tag="ACHKA1_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5678915.1"
                     /translation="MLFIHQIMLYFCTAVCALYLISGGYSVIRNYFQRKIDERAAEKI
                     SQKSVQQNNQNMP"
     gene            complement(32085..34130)
                     /locus_tag="ACHKA1_001235"
     CDS             complement(32085..34130)
                     /locus_tag="ACHKA1_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5678916.1"
                     /translation="MVPKHYQLVTAMTNKELAEKLAPMLREGWGLLGGPVSASEPGAP
                     FYLMQALVTDRDLPSAIPVPEGEEKPVPGTTDGYNTGHTTEPEFYYVVPLAGQSNGMA
                     YGEGLPLPDTFDSPDPRIKQLARRSTVTPGGAACTYNDIIPADHCLHDVQDMSSLNHP
                     NADLSKGQYGCVGQGLHIAKKLLAFIPKNAGILLVPCCRGGSAFTSGAEGAFSESSGA
                     TAESSLWGVGKPLYNDLLTRTKAALDKNPKNVLLSVVWMLGEFDLKSATYAQHPQLFL
                     NMVNQFRADLSAYAAQCTGGNASLVPWICGDTTYIWKQQYTSQYPVVYGAYQNKEAQK
                     IYFVPLMTDEKGANTPTNLPAEDPDLAEAGYYGAASRSNGNWTSSDRATHFSSWARRG
                     IISDRLATAILMHAGKTVAFLKGAPAPFSVVAVPPATAGSDSTTTESPQPSTPAPQPG
                     AGTQSSPARTTAVAYRADAQEGKLKEQGWTGADGKASVVNDSAAESGKAMRVEKVQGQ
                     QTAWKIAHDVPASKAAELLQKGGEIAFRFKIPEGVQLAANRFAMALYWPVSQLPENVV
                     PEGSTGSNMLAAFFIQSDSANLNLMQHKQQNAKLGTFGAFNHNWHNVVFRFAGNNSIS
                     VTPVINGQTQAAFNLAKCPASGVENDKLLITDITSNQDTYPVLIEYVTVDINNSNAA"
     gene            complement(34141..34272)
                     /locus_tag="ACHKA1_001236"
     CDS             complement(34141..34272)
                     /locus_tag="ACHKA1_001236"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678917.1"
                     /translation="MIWADVADWGDRLVDALDSCNADKAAIAGLERQRTARMNTHEK"
     gene            complement(34259..34684)
                     /locus_tag="ACHKA1_001237"
     CDS             complement(34259..34684)
                     /locus_tag="ACHKA1_001237"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFO5678918.1"
                     /translation="MKLNIILCALLLTISLQAMVVIRAYRSGQESTRYEALEKAVKES
                     NTTVRELLKATAETQSALAAIRREDQERAARGEERRETMRKNLQADECARTSPPADVA
                     DSLQQHASAAENRRHLRERAGKSDQTDAGTGAAATHDMG"
     gene            complement(34681..35223)
                     /locus_tag="ACHKA1_001238"
     CDS             complement(34681..35223)
                     /locus_tag="ACHKA1_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5678919.1"
                     /translation="MAGPKRYAVAAIVALGISLAPGELRTSREAQIKIATWEECRARP
                     YRDLVGVGTVGCGSTGNVENRLYTQEEVAGRWINDMRRAENCITQNFRGQQMPQSAFE
                     AMTDAAFNLGCRNLMWFKNKNGTPQRTTIWKHAQTRQWRLMCYRLTDFVNSGGTRTQG
                     LVNRRNDFKNWCLKDAGAQP"
     gene            complement(35210..35494)
                     /locus_tag="ACHKA1_001239"
     CDS             complement(35210..35494)
                     /locus_tag="ACHKA1_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5678920.1"
                     /translation="MFQRLCDHITFWASVTTAGIGVMTLSEKIAVAGFVLGTLSLLRA
                     WLHRRRMEIAQARRNELIEKILMQSEGRELNDAERRAMMLLKQDEHDGRT"
     gene            complement(35497..35697)
                     /locus_tag="ACHKA1_001240"
     CDS             complement(35497..35697)
                     /locus_tag="ACHKA1_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO5678921.1"
                     /translation="MKVMALDGDTVDLLCQRHYGTTQGVTEAVLAANPGISAVMFLHA
                     GQEVELPELQGQTRREMIQLWG"
     gene            complement(35697..36191)
                     /locus_tag="ACHKA1_001241"
     CDS             complement(35697..36191)
                     /locus_tag="ACHKA1_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO5678922.1"
                     /translation="MSLIARPRVNSADTDTTDTDDGDAMVSAGDFWPAISLHEIRLAS
                     RIPGRTTTTRLLHAATEAVAHVIAELELWKRERMAEGAASLADVPATQVNGESVHVHR
                     YRRAVFALTRAFVLERTRDVDTTDKGDRRADALDMQVEDLWRDARWAISDIRGVTRIY
                     AELV"
     gene            complement(36296..37093)
                     /gene="gpM"
                     /locus_tag="ACHKA1_001242"
     CDS             complement(36296..37093)
                     /gene="gpM"
                     /locus_tag="ACHKA1_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO5678923.1"
                     /translation="MLTPAQRYVQQAVARNRVTMNARETQRERTAHEQVLHQLRLAQM
                     QLKGIQSNVARAELKKHLLPDFEGWIEGTLAGDSGRQDEVITTMMVWAIDCRDYPLAL
                     RIGEYVIRHGLALPDNFGRDAATVLAEEITDAVLTQATTDSEADLSVYTAMLDELNHL
                     VSEQDMPDQVRSKLHKARAFSRRASSSPDDLRLSLSLLREAMRINPASGVKREIATLE
                     RAVRKLADGNQVVPASTTGRKTTTGGKKSSAKKKSAASQKKAARKTN"
     gene            complement(37134..38234)
                     /locus_tag="ACHKA1_001243"
     CDS             complement(37134..38234)
                     /locus_tag="ACHKA1_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO5678924.1"
                     /translation="MARLSLSREGRDSLERYMIQQASLAGITTERLGKTFSVDPSIQQ
                     KMENAIKESAGLLQEINFIGVDDQEGEKVLIDTSGTIASTNATSDGVKRRNPASVADL
                     AARRYRCEQVNYDTFISYAQIDAWSSQKNFQQLLSAQITRQIALDRIMIGFNGESHAL
                     ISDRATNPLLQDVNPGWLKHIRDKAATRVVKGMTLTRRDDENKLVAKGDYGNPDAMVH
                     DIRSSVLDEWHKDAPDLVVLMGRDLFNTLRLPMINAISTTNPNTELVAGQLIVASRTV
                     GGLQTYLAPYFPKDAMLITSLKNLSIYFQKGSLRRFIREEPEYNRIATYQSMNDCYVV
                     EDYGKCALIEGITFAGEDPDNNAESGQPQPEA"
     gene            complement(38252..39088)
                     /locus_tag="ACHKA1_001244"
     CDS             complement(38252..39088)
                     /locus_tag="ACHKA1_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO5678925.1"
                     /translation="MGSKNTPERKKFRVAVSGSTIDGREISGEMLRQAAENYDPSVYA
                     SRVNIEHVLSMWPSSELCAVGDLIALSTDEITEGKLAGRTALYAEIEPTDRMKNMIAD
                     GKKVYSSIELLPELDAVKGPYIVGLAMTDTPASLGTERLKFAAQQRASIMQFSKCTTE
                     PSMFTVAMEAEMLTTTEQRTEESRQWFSRVMALISKTRTADGEQFSQLRDVVENIASD
                     HADLIDRFSTLEQKHALDKTALDALSHELAALKAQLRNQDADPQQRFTSAGGTDQTLA
                     EF"
     gene            39244..40977
                     /locus_tag="ACHKA1_001245"
     CDS             39244..40977
                     /locus_tag="ACHKA1_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO5678926.1"
                     /translation="MIQDVFIRMRAKQLYWQGYTPADIAQLMGINQNTIYSWKKRDEW
                     DATPPIQRVTQSIDARLIQLTAKPDKSGGDFKEIDLLTRQLKKLNDGQGEQAVSGKKP
                     RRRKLKNHFTEEQIVALREKILPSLAWHQREWYDNRHHRNRMILKSRQIGATWYFARE
                     ALLDALRDDVSAGYQRNQIFLSASRRQAYQFKHFIQQAALEVDVELKGGDKIVLSNGA
                     ELHFLGTSAATAQSYTGHLKFDEFFWVSNFVELRKVAGAMATLEGLTRTYFSTPSGET
                     HEAYQFWTGDRWNSEQPRSKRSEFDVSHAHLKGGVLFPDETWRQIVTIEDAVANGWRY
                     TNIGVIRSENTESEFRNLYMCEFVRDGEAAFSLSALTGCGVDGYDEWPDWKPFAARPM
                     GVREVWLGYDANGSSGKGDCGALSVCVPPLVAGGRFRTVETVRVQGMEFEQQAALIEE
                     YTQKYNVKHIGIDVTGGNGEGVYQIVKKFFPMAVPYNFNIASKRALVMKMLQVVRAGR
                     WEYDRSERDLIQAFASVRKIQTPGGYITYDTDRSRGVSHGDLAWATMLAVINEPIGAE
                     REDNRGFAMEF"
     gene            40983..42026
                     /locus_tag="ACHKA1_001246"
     CDS             40983..42026
                     /locus_tag="ACHKA1_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5678927.1"
                     /translation="MSKRKKYTGKRGQDSLHDALKSTPALSAFTFDGPYEVSRMDLLD
                     NMSCADNGRYFDTPMDWNAIARASRRASWHQSALYFKRNALNGCFVPHPLLSRQAFSA
                     FALDWFVFGNAYLEVRRNRLGEPLLLRPALAKYTRRGSDLDTYWYLNDDGTEFSFRKG
                     TVCHVLNPDINQEIYGMPEYIGGLLSVSLSNSADTFRKLYYDNGSHAGCIIYVGTSQA
                     NAESVEAIKKTLTDSRGRGAFKNLFLHAPGGGKDGVQILPFQQITAKDEFLNIKGSAR
                     DDILAAHRVPPQLMGAMPDGNAAFGDVEKAARVFFINELQPVMEAMKHVNEWLGVEVM
                     RFNPYALLLDNAS"
     gene            complement(42092..42595)
                     /locus_tag="ACHKA1_001247"
     CDS             complement(42092..42595)
                     /locus_tag="ACHKA1_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678928.1"
                     /translation="MSHFRDLYLANKKSRDARREVTGILRNQAEKLVAFYEQWLELPS
                     ETWEDKDGEHHRYVDTGFPCRNDRDFRPFPVNMIATDTDNIFRMAVRTWVDSHDEGES
                     VNIVLAMELHSVGKDGVSLRVRVEDDTSIRVVINGDGDREWLPIVNSMKIHIISKLQR
                     RTPSAFR"
     gene            complement(43131..43508)
                     /locus_tag="ACHKA1_001248"
     CDS             complement(43131..43508)
                     /locus_tag="ACHKA1_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5678929.1"
                     /translation="MKARIGIIPEKVLRQRMLDIAKGKLKPEPDEPRVWFSSLNALGQ
                     ALSNENIALLRIMDAEKPQTMTELASLSGRQLSNLSATLKMLSRYGFVSLEKQGNCVQ
                     PKALFTDFEIIIDPSVGEAHRAA"
     gene            complement(43505..43879)
                     /locus_tag="ACHKA1_001249"
     CDS             complement(43505..43879)
                     /locus_tag="ACHKA1_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015834182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HFO5678930.1"
                     /translation="MKNNDHGLNTLLDMHGYQHHMDNGYWWKIEAYRVSPSQFRPHGI
                     RYNLTLHDPYNSRVFGMDNAHGIKPPKKGKFTGRITVYDHIHRTSSDKGYPYEFTSAE
                     QLLLDFFNHVDRVIAEQQRGKK"
     gene            complement(44134..44451)
                     /locus_tag="ACHKA1_001250"
     CDS             complement(44134..44451)
                     /locus_tag="ACHKA1_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5678931.1"
                     /translation="MTTQIATRGRLAEDPTTRKSSRGTDVTAARLAISLPGHGAEQST
                     TMWLYLIAFGKQHEELAKHKKGDLVQVHGNLQVNRYQDKYGQMKEGWQLLAQSIISSR
                     TAI"
     gene            complement(44475..44822)
                     /locus_tag="ACHKA1_001251"
     CDS             complement(44475..44822)
                     /locus_tag="ACHKA1_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO5678932.1"
                     /translation="MKTPGMTGKEIILRYLETHARFSAPELVEKLGVKRTVINQAAGK
                     MVREGLLVVDGYTDKITHYRKPTEEERLELAQRAEWQQEKSVIEVCKRSEVMKRILFI
                     YGAGGDLPVISVQ"
     gene            complement(44819..45013)
                     /locus_tag="ACHKA1_001252"
     CDS             complement(44819..45013)
                     /locus_tag="ACHKA1_001252"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678933.1"
                     /translation="MSTRQQHTINSTRAISDRDLNSMIESAQQLLSRQNPVVEETWWE
                     NLLAALTELRERRNNEGATK"
     gene            complement(45033..45434)
                     /locus_tag="ACHKA1_001253"
     CDS             complement(45033..45434)
                     /locus_tag="ACHKA1_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678934.1"
                     /translation="MTRFNCTLQQREFSVVMTKRLPALTLRGLIRRPQPGDQLEITIA
                     RPDRSTESMTADILNARPWGDSGDFIAALRLRNTGAPEPLTPAGRIMKAYHEKDIRKH
                     NEDLARAMKRINRKAAIASYVHSAARKQHRK"
     gene            complement(45431..47944)
                     /locus_tag="ACHKA1_001254"
     CDS             complement(45431..47944)
                     /locus_tag="ACHKA1_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO5678935.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RTDARLDEETVAFLTTTDEGKRINSHLHHDEERRIRLEKLTRHKRENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATAFSTALERACDEVIEKTG
                     RGDDDLLTWLLTYQPLAHMALKLGITPPCWPSLEIRPDRRTEPDPERVPGAVMRLSCA
                     TWWNSQLRHLRDIWREELLRAAGLVSRKTSVYISHEALLDWREKRQRTRDFLKAWDIE
                     NEEGERFSLEDIYWAGLSNPRNRRNEMMTCARGMEQVAEARGDVAFFVTVTAPSRFHS
                     VNDDGSLNPKYCGATVRDASDYLVYRFFAAVRRTIDKAGLGWYGVRTVEPHHDGTPHW
                     HMLVFTSQENEARITEIMRNAAIREDRAELGDDISPRFKCEKIDPTKGTPTSYIATYI
                     GKNLDASAFMGNDPKTGKPYVDKESGKTMAETVENAIGWAGLHRIHQFQFFGIPPRQV
                     WRELRRLAGQMARNPAAPQRLDHDDIDAILAAADVGCFATYITRQGGVLIPRNTYLVR
                     TAYETAEEANDYGEFPQRIYGVRAPSLGERYTICTHPDTWKLVRRKPENEDRTDEGFD
                     VECGFAAPWTRGNNCRHDEKTNNETDKTPPPEGRKEQKLIVPEGDPGEWLRSLTPVQR
                     KQLTQQLKNDQKSTKTANSGGFPAPEGVVYAGAALAKPDMTTRLIASTLSTTGEELHS
                     SAALSVYRGARVRLPDGRIVHWDESTGSLKEVAQLKGDE"
     gene            complement(47941..48351)
                     /locus_tag="ACHKA1_001255"
     CDS             complement(47941..48351)
                     /locus_tag="ACHKA1_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678936.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPRLASRLNDWHDYYRRACKQAAR
                     HVTTYGDFSFTVDDRYRVDVKGDGFGLFHINGTDSTAISWSSSEMTLVLEMTTHSVAV
                     KGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            complement(48420..48647)
                     /locus_tag="ACHKA1_001256"
     CDS             complement(48420..48647)
                     /locus_tag="ACHKA1_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO5678937.1"
                     /translation="MANRKQQRARAERLHIHTEINRRLHRAASVSFIMQSNVLHRLND
                     GIAAGYYAAVFSYLAEDLAELQDLINQQHHH"
     gene            complement(48578..48961)
                     /locus_tag="ACHKA1_001257"
                     /pseudo
     CDS             complement(48578..48961)
                     /locus_tag="ACHKA1_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            48971..49495
                     /locus_tag="ACHKA1_001258"
     CDS             48971..49495
                     /locus_tag="ACHKA1_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678938.1"
                     /translation="MWRINITWQLVIVCIRGVFLMSDKLPSDYDILFDLWYSYWLERI
                     TCVFHRRVSAALRISAFLVGVAAFVLCDFVVLVLFSVVLFLLWQCMAASFERRAEKAE
                     WQAVSYQRLISQRHVISKECMIARLSFVEESDSPIMECMINLAWNKACEAVGSSSRNH
                     VSISGRLLSRLCGF"
     gene            complement(49469..49819)
                     /locus_tag="ACHKA1_001259"
     CDS             complement(49469..49819)
                     /locus_tag="ACHKA1_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO5678939.1"
                     /translation="MAVIYIAGPMTGYKDDNRLEFCLADLKLRYAGNIVLNPAVLPDG
                     LSQQQYMSICIPMLMCADAIYLLDGWEESAGARAEYAMALKLNMPVSFPENRAAGETV
                     AQLLGKSKAAQATE"
     gene            complement(49810..50028)
                     /locus_tag="ACHKA1_001260"
     CDS             complement(49810..50028)
                     /locus_tag="ACHKA1_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678940.1"
                     /translation="MIQESTVIRFTANGRQYEVDESLIDQGMTRQDSRNPEMHHIRLI
                     NGSHFCATNMEEVRVLTATRNAGGKAWP"
     gene            complement(50025..50261)
                     /locus_tag="ACHKA1_001261"
     CDS             complement(50025..50261)
                     /locus_tag="ACHKA1_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678941.1"
                     /translation="MNSERNKALLRGLRHIAHAHEETAKVAAAVGKPKVVISSSISLG
                     ITMEAIRVIQDQESEIAALKKQLNEKSAGQRGQQ"
     gene            complement(50297..51277)
                     /locus_tag="ACHKA1_001262"
     CDS             complement(50297..51277)
                     /locus_tag="ACHKA1_001262"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFO5678942.1"
                     /translation="MSKELLSGTPLKWVGGKSRIMNTLKVHLPEADCLVEPFVGGASV
                     FMNTSYKRYILGDSNGALINFWRAARDNTDSLIDEAKTLFLNHNNRGDYLIIRETFNA
                     ENHAFLSSRTGISDRRELRLAAMFLYLNRHCFNGLYRVNGKGDFNVPFGCYRKPYFPE
                     HEIRAFADKANSTRTLLIHADFKDTLNSASHLFGMGNTLCVYCDPPYLQFSGRDNFTA
                     YGKPFDEHEHVRLRAALDRLSQETGGMTSITISNSDTPETRRIYQGYRMNCIQAPRSV
                     GARTREPAPEVIATLRQCDACGHYGGGYCPDCGPVTGDATYCVMFTPAST"
     gene            complement(51510..52091)
                     /locus_tag="ACHKA1_001263"
     CDS             complement(51510..52091)
                     /locus_tag="ACHKA1_001263"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO5678943.1"
                     /translation="MNHLMIDTETLGNGPDAVIFAIGAVFFDPFTGKLGKQFEKFIDP
                     VDSERNGGTVSATTAVWWAGQSVEARACLRNAEGTELAAVTEFLAFISRNMHDESPGN
                     SLTIWCKGASFDFPILRSAIKRTTGEKSIPWCYWDERCMRPLIAMAESTGWRMPGRSG
                     KEAIAHTALGDAIYQAKVVSEIWQRFTTPFLNI"
     gene            complement(52088..52321)
                     /locus_tag="ACHKA1_001264"
     CDS             complement(52088..52321)
                     /locus_tag="ACHKA1_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678944.1"
                     /translation="MRTAESPGREITARPANHHAHISMAARKAERAHICELLRELTET
                     AKEKHTSPLETCSVIERLIAMLTSQDEPKEPVQ"
     gene            complement(52536..52661)
                     /locus_tag="ACHKA1_001265"
     CDS             complement(52536..52661)
                     /locus_tag="ACHKA1_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678945.1"
                     /translation="MSIFLSWVILIISVLMAIGIMRIIHTLKDIELFFTGDKRRK"
     gene            complement(52658..52900)
                     /locus_tag="ACHKA1_001266"
     CDS             complement(52658..52900)
                     /locus_tag="ACHKA1_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO5678946.1"
                     /translation="MIEAYKILINNALQRYHFELANRGVTKDESLRNSLRGAIMGLYN
                     CALGSDDREALEELKSIMTELFEGEIVEPYFTGVAA"
     gene            complement(52919..53263)
                     /locus_tag="ACHKA1_001267"
     CDS             complement(52919..53263)
                     /locus_tag="ACHKA1_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO5678947.1"
                     /translation="MKKPGNFSQQNFRTSAERHVARQHFRKNSATTTTEIHATPDGHP
                     VKQTGKHTWAIGDTGIVIHKSFRSPVTGRFNFTLTRGDDYFGQDFTFFEALRTADRLI
                     SGLRFQYPGSKH"
     gene            complement(53416..53679)
                     /locus_tag="ACHKA1_001268"
     CDS             complement(53416..53679)
                     /locus_tag="ACHKA1_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678948.1"
                     /translation="MNKEKSPDYFVKRLQDATESMLIALNNYSYEEAVTVLEWIKEKI
                     TEDSIVKMQSINISRDKTFPLRIPPKRRLVYSIPVSMQCKLPK"
     gene            53725..54135
                     /locus_tag="ACHKA1_001269"
     CDS             53725..54135
                     /locus_tag="ACHKA1_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5678949.1"
                     /translation="MRAAERELARSEGFHSFVDARAHAELFRIGARSPVILRSVKKCA
                     QSLVNDKYHLVFLVTESTSGFCLSLSYPENIHLYDEVFESFEGAESFAQHLSYDVFEL
                     ADIYDCWGSLIHPLQHSRFYRNVFDIDDDHPSAW"
     gene            complement(54220..54744)
                     /locus_tag="ACHKA1_001270"
     CDS             complement(54220..54744)
                     /locus_tag="ACHKA1_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5678950.1"
                     /translation="MRKSIADKELSDTQALQENDSITESVGAANDDEHQEEKKKRTYK
                     KGATLRLDGPVAGLCSLEKGAAYIGLTKAALRVALQRNQMPGHKTRSNPEDENSDGVW
                     WFNAREWDKLADELPECEPPEWHNWKSYWTYDRQKRKFSPANKEDCQTINGKRVYTGR
                     KSKLEQHQYSGGNQ"
     gene            54848..55189
                     /locus_tag="ACHKA1_001271"
     CDS             54848..55189
                     /locus_tag="ACHKA1_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5678951.1"
                     /translation="MVNMSRDYAKKLKAIRNAENLTQSQFSQVTGVSVGTIGNYESGY
                     KPARIEVVEKVIQVERFEKYTMWILHDKTFPAAGQVAPALSLDGSIQSEGDQVSIGIT
                     QKSPRSRRNAG"
     gene            55259..56251
                     /locus_tag="ACHKA1_001272"
     CDS             55259..56251
                     /locus_tag="ACHKA1_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5678952.1"
                     /translation="MSIKQLKDGRYKVDVRPQGTEGKRIRKIFTLKSKAQEFERYVLQ
                     NFHDKPWQAKPADQRRLSELIDAWWVLDGRNQPYGDTYRVRLEKINREMRDPRASEMT
                     RKFMLGYRSDKLQAGLMPSSVNRDLCALSAMFTSLIDAEVFHNENPVRGIRKLKVRNT
                     EMAFLSDDEIDRLLERLEGDARRVAVLCLSTGARWGEAAGLRGEHIVGNRVMFFNTKN
                     GKSRAVPVSDTVLSLIKTRKTGLLYQADYIRFRDILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHATIQQTMTYAHFSPDFLQDAISFNPLADSVHKLSID"
     gene            56342..56875
                     /locus_tag="ACHKA1_001273"
                     /pseudo
     CDS             56342..56875
                     /locus_tag="ACHKA1_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
     gene            56872..57444
                     /locus_tag="ACHKA1_001274"
     CDS             56872..57444
                     /locus_tag="ACHKA1_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5678953.1"
                     /translation="MKTGAEAIRALATQLPMFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            57679..60123
                     /gene="dmsA"
                     /locus_tag="ACHKA1_001275"
     CDS             57679..60123
                     /gene="dmsA"
                     /locus_tag="ACHKA1_001275"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO5678954.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            60134..60751
                     /gene="dmsB"
                     /locus_tag="ACHKA1_001276"
     CDS             60134..60751
                     /gene="dmsB"
                     /locus_tag="ACHKA1_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO5678955.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            60753..61616
                     /gene="dmsC"
                     /locus_tag="ACHKA1_001277"
     CDS             60753..61616
                     /gene="dmsC"
                     /locus_tag="ACHKA1_001277"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO5678956.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(61652..62278)
                     /gene="ycaC"
                     /locus_tag="ACHKA1_001278"
     CDS             complement(61652..62278)
                     /gene="ycaC"
                     /locus_tag="ACHKA1_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO5678957.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            62593..63741
                     /gene="ycaD"
                     /locus_tag="ACHKA1_001279"
     CDS             62593..63741
                     /gene="ycaD"
                     /locus_tag="ACHKA1_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5678958.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            63951..65381
                     /gene="ycaM"
                     /locus_tag="ACHKA1_001280"
     CDS             63951..65381
                     /gene="ycaM"
                     /locus_tag="ACHKA1_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO5678959.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIHNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(65382..65492)
                     /locus_tag="ACHKA1_001281"
                     /pseudo
     CDS             complement(65382..65492)
                     /locus_tag="ACHKA1_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(65591..66331)
                     /gene="pflA"
                     /locus_tag="ACHKA1_001282"
     CDS             complement(65591..66331)
                     /gene="pflA"
                     /locus_tag="ACHKA1_001282"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO5678960.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(66523..68805)
                     /gene="pflB"
                     /locus_tag="ACHKA1_001283"
     CDS             complement(66523..68805)
                     /gene="pflB"
                     /locus_tag="ACHKA1_001283"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO5678961.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(68860..69717)
                     /gene="focA"
                     /locus_tag="ACHKA1_001284"
     CDS             complement(68860..69717)
                     /gene="focA"
                     /locus_tag="ACHKA1_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO5678962.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT        15417 a        17765 c        18978 g        17662 t
ORIGIN      
        1 ggatgtacac aatgagacag aagagctatg cgactgccgc ttctacttcg acgggcacaa
       61 taacactggc gtgattgcct tttggcccct ggtggacatc aaactgaacg gattgtccag
      121 cttttagcgt tctgtaacca tccatctgaa tggtggaata atgagcgaaa atatcttcgc
      181 cgccgccttc agggcagatg aaaccaaacc ctttggcatt gttgaaccac ttaacagtac
      241 ccttttccat gcttcgacat ccttcgcaaa tcttatacaa gtaagatgga ataaaccggg
      301 gtcagagagg gggctgttca aaacctcgcc aactctagaa atacaattta gagaattagg
      361 gcgagccgtc aagcatttga caggggacaa ggggcaggta tgaatcaaaa agttgaagca
      421 gttaacgcta ttgacaggaa tgtgacagat gtcgctgatg ccaacgatag atgatagtta
      481 tctatcatgt ggagtagatt ggtcaggcaa ataagctctt gtcagcggca gggcgttctg
      541 ccgataaccg taaccgaaga tgataactga caatgggtaa aacgaacgac tggctggact
      601 ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa accgccatct atgtataaag
      661 tgatattagt caatgatgat tacactccga tggagtttgt tattgacgtg ttacaaaaat
      721 tcttttctta tgatgtagaa cgtgcaacgc aattgatgct cgctgttcac taccagggga
      781 aggccatttg cggagtcttt accgccgagg ttgcagaaac caaagtggcg atggtgaaca
      841 agtacgcgag ggagaatgag catccattgc tgtgtacgct agaaaaagcc tgaatgcagg
      901 cataaaaatt gggggaggtg cctatgctca atcaagaact ggaactcagt ttaaatatgg
      961 ctttcgccag agcgcgcgag caccgtcatg agtttatgac cgtcgagcac ttgttactgg
     1021 cgctgctcag taacccatct gcccgggagg cgctggaagc gtgttctgtg gatttggttg
     1081 cgctccgtca ggaactggaa gcctttattg aacaaaccac acccgttctg cctgccagtg
     1141 aagaggagcg cgacacacag ccgacgctga gttttcagcg tgtactgcaa cgtgcggtct
     1201 tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc aaacgttctg gtcgctatct
     1261 ttagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa acatgaagtc agccgtctcg
     1321 atgtggtgaa ctttatctct catggcacgc gtaaagacga gccgacacag tcttctgatc
     1381 ctggcagcca gccaaacagc gaagaacaag ctggtgggga ggaacgtatg gagaatttca
     1441 cgacgaacct gaatcagctt gcgcgtgtgg gcggaatcga cccactgatt ggtcgtgaga
     1501 aggagcttga gcgtgctatt caggttctct gccgtcgccg taaaaacaac ccgctgctgg
     1561 tgggggaatc tggtgttggt aaaactgcga ttgcggaagg tcttgcctgg cgaattgttc
     1621 agggtgatgt gccggaagtg atggctgact gtacgattta ctctctcgat atcggttctc
     1681 tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg ttttaaagcg ttgctcaagc
     1741 agctggagca ggacactaac agcatcctgt ttattgatga gatccacacc attatcggtg
     1801 cgggtgcagc gtctggtggt caggtcgatg cggctaacct aatcaaaccg ttgctctcca
     1861 gcggtaaaat tcgcgtaatt ggttcgacaa cctatcagga gttcagcaac attttcgaga
     1921 aagaccgtgc tctggcgcgt cgcttccaga aaattgatat tactgaaccg tcgatcgaag
     1981 aaactgttca aatcatcaat ggcctgaaac cgaagtatga agcgcaccac gacgtgcgtt
     2041 ataccgcaaa agcggtgcgt gcggcggtag agctggcggt gaaatacatt aacgatcgtc
     2101 atctgccgga taaagcgatt gatgttatcg acgaagcggg cgctcgcgca cgcctgatgc
     2161 cggtaagcaa acgcaagaaa accgttaatg tggcggatat tgagtccgtg gtggcccgta
     2221 ttgcacgcat tccagagaag agtgtttctc agagtgatcg tgataccctg aaaaacctcg
     2281 gcgatcgcct gaaaatgctg gtcttcggtc aggataaagc cattgaggcg ctgactgaag
     2341 ccattaagat ggcgcgtgca ggtttaggtc acgaacataa accggtcggt tcgttcctgt
     2401 ttgccggtcc taccggggtc gggaaaacag aggtgacggt acagctttcg aaagcgttgg
     2461 gcattgagct tctgcgcttt gatatgtccg agtatatgga acgccatacc gtcagccgtc
     2521 tgattggtgc gcctccggga tacgttggtt ttgatcaggg cggtttgctg accgatgcgg
     2581 taatcaagca tccacatgcg gtgctgctgc tggacgaaat cgagaaagcg catccagacg
     2641 tgttcaatat tctgttgcag gtgatggaca acggtacgct gaccgataac aacggacgca
     2701 aagcggactt ccgtaacgtg gtgctggtga tgaccactaa cgccggggta cgggaaactg
     2761 agcgcaaatc cattggtctt atccaccagg ataacagcac cgatgcgatg gaagagatca
     2821 agaagatctt tacaccggaa ttccgtaacc gtctcgacaa cattatctgg ttcgatcatc
     2881 tgtcaactga cgtgatccat caggtggtgg ataaattcat cgtcgagttg caggttcagc
     2941 tggatcagaa aggtgtttct ctggaagtga gccaggaagc gcgtaactgg ctggccgaga
     3001 aaggttacga ccgggcaatg ggcgcacgtc cgatggcgcg tgtcatccag gacaacctga
     3061 aaaaaccgct tgccaacgaa ctgctgtttg gttcgctggt ggacggcggt caggtcaccg
     3121 tcgcgctgga taaagagaaa aatgagctga cttacggatt ccagagtgca caaaagcaca
     3181 aggcggaagc agcgcattaa tctgattgtc aggtaggttg gtgaagtccg taatctcgtc
     3241 aggggttacg gactttttat ttatggggtg gaacggtgaa aaccctattt ttggaggtga
     3301 aggtaagttg ttgataatta gtgctgctgg aaggtaagga taaaaaaggg tgctgcagga
     3361 gaatgggatg gttttgcttt attaacaacg ggctaaacgt gtagtatttg agttcactgc
     3421 cgtacaggca gcttagaaaa acctaccgtc ttggctagcg gttgcagcga acgttcactg
     3481 ccgtacaggc agcttagaaa ttcactggta acatactcca cccgcccacc atgttcactg
     3541 ccgtacaggc agataaaatg cgaaaaaaaa agctcgcact ttcgtacgag ctcttcttta
     3601 aatatggcgg tgaggggggg attcgaaccc ccgatacgtt gccgtataca cactttccag
     3661 gcgtgctcct tcagccactc ggacacctca ccaaattgtt ttgttgcctg acctcatggg
     3721 gggcaacggg gcgctactat agggagttgg agtaaaacgg tcaagaagaa ttttaatgat
     3781 aattattgtt tgctcatact gtaaacaagt tgtgcagtat atctacatcg agacaagtta
     3841 cggacttata cttccaaagt acttcataca tatcacaaaa taaaaaggcc ggttaaaccg
     3901 accttttact cgttctttct cttcgcccat caggcggtaa aacaatcagc gactacggaa
     3961 gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca acagtcactt tgtcgcccgt
     4021 caggatgcgg atgtagtttt tgcgcatttt accggagatg tgtgcagtaa ccacgtgacc
     4081 gttttctaac tctacgcgga acatggtatt aggcaacgtt tcaagaacgg taccttgcat
     4141 ttcaatattg tcttctttgg ccatctaatc ctctggggta tcactaccgt aatttgaacc
     4201 ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt gcgcgctaaa tcgcaacaaa
     4261 caggttcggc acattacccc gaaaacacac ggctaagccg caccaaaagc gcaacgtata
     4321 agggagcggt gagataaacg atgggcgtta cctgacgcga aaaattcctt atcggcagcg
     4381 gggtaatgag cgtaaccaac tctgcgaccg caattataac actctgggga gaaatgtgcc
     4441 gaaaacattc attcttgtgg tgaaaacaag catcgtggta cccagaaatt attcggcaat
     4501 cgtccgaggc gcatttgatt gagataattc agataatccc gacggggaat ttcgcaggca
     4561 ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga taagcttacc gccatgaccg
     4621 ataaattcct cacagaatac cagaagcgcc gttttagacg cattttccat ccggctgaac
     4681 atggactcgc cacaaaatag cgttccctgg gccacgccgt acataccgcc gacaagctca
     4741 tcttcacgcc agacttcaat ggagtgggca tgaccgagtt cgtgaaggcg atggtaggct
     4801 tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat cgctggcaca gccttcaatg
     4861 acctgaccaa aagcgtaatt catcgtgaca cgatagggcg agcgtttatg aaatcgcttc
     4921 atactacggc tgatatgcag tgattctggc catagcaccg cgcggggatc gggcgaccac
     4981 cagaggatgg ggtcgcctgg agaaaaccac ggaaaaatac cacgctggta agccattaac
     5041 aggcgcgcag ggctaagatc gcccccaagt gccagcaggc cgttaggctc acgtaatgcg
     5101 ccttccgggg aagggaaggc tattgaatgg cgagaaagct gaaccaggcg catgaccgca
     5161 aaactccacg caagtcggat cgttcaataa tagcttacaa accctgcttg aactggtaat
     5221 aacgcccctg tctggcaagc agttctgcgt gagtaccttg ctcaataatt tgcccgttgt
     5281 ccatcactat tatttgttgg aaacgagaga gcccgcgaag tcgatgggtg accattaaca
     5341 ccgttttctc acgcatcatt tctgcaagca attcaaggat ctggctttcg gttgtggcat
     5401 ctaagccttc ggtaggttca tccagcaaca ccagtggcgc atcatgtaac agcgcacggg
     5461 cgatagccag acggcgcagt tcaccaccgg agagctggcg tccgccttca cctaaccaac
     5521 tgttgagacc tgcatcctcg agcagcttct ccagaccaac gcgacgcaag atcaccgcca
     5581 gagcctcatc actactgcca ggcgaggcga gtaacagatt atcacgcagc gtggcgctaa
     5641 acagatgcac tcgctgagga acaacgctga tggtctgtcg tagagccgct tcattcaggc
     5701 tggctatggg gctatcgtta agcaaaatct cgccctgttg cgggtcccat gcgcgggtca
     5761 gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc gagaatcgca atatgttctc
     5821 cggtgtttac ctgaagagaa atccctttaa gtgcctgttg ggattgctcc ggataagtga
     5881 actgaacatc ccgtaacgtc agcgaaacgc gatcggcaac acgagtttgg gtatcaggga
     5941 aggtgacctc cggtttttga tccgttaagt cagtgatccg aacggcagag gcaatgactt
     6001 gccccagatg ctgaaatgca cctgttactg gtgccagtgc ttcaaacgcg gctaacgcgc
     6061 agaagacaaa cagggcaatt aacgcgccag gttgagcatt gccgccaacg ccgccagacg
     6121 ccatccacag catcaggatc accgctaacg cgccaatgag cagcattatc gcttgcgaca
     6181 atgcggtcag ttcagattga cggcgttgcg cttccagcca ttgaatttct gtattctcta
     6241 gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag ctcagcttgc ccttgcagcc
     6301 aggccgtcag ttgttggcga tactgtccgc gaagatgagt cagattttgc ccggtgcttt
     6361 ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt cagtaacata atgccgccca
     6421 gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat tgtcaccacc ataatcacca
     6481 caaaagcgcc caccagcggc gagataacgc gcaggtaaag atgatcgagc gtatcaacat
     6541 ccgccaccac gcgattgagc aattcgccct gacgatagcg cgccagtccg gcaggggaga
     6601 ggggcaacaa tttgctgaag gtgtaaatgc gcagatgctg caacacgcgg aaagtcgcgt
     6661 cgtgacttac cagacgttca aaataacgcc cggcagtacg ggtgattgct gcaccacgca
     6721 cgcccgcagc gggtagcata tagttgaagc tgtacagtcc ggcaaccccc gcaaccgctg
     6781 aggccgagag gaaccagccg gaaagtgtca acagaccgat actggcgagc agcgtcacaa
     6841 ttgccagcac aataccaaga cttaacatcc atttatgacg tttatacagt gccagatagg
     6901 gtagcaaagc gcgcatttaa atctcctcct gacgatgggc cagtaatgtg gcgaatgggc
     6961 caccagccac acttaattcc gcgtaacgtc cttgctcaat aatccggcca tcctgcataa
     7021 cccaaatgac atcccagtca gcaagatctt ctaactggtg ggtgaccatt aacgttgtct
     7081 ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg ttcactgtga gcatcaaggc
     7141 tggcagcggg ttcatccaac agtaatagcg aacagggatt tagtaacgca cgggccaccg
     7201 ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg gtcgccaaca ggcgtatcaa
     7261 cgccttgtgg caggagcggt agaaactcgc tgacccaggc gttatccagc gctgcttgta
     7321 attcttgttc gctggcatca ggtcgcgcca gtagtacgtt atcccgcaat gttgctgccg
     7381 gtaattgtgg gttttgccca acccaggaga gatgtttacg ccatgattct ggtgataaat
     7441 cgcgtaattc tatcccgttg attcgtaatg atccctgata tgagagaaaa ccagaaagcg
     7501 cgttcagcag tgagctttta cctgaaccgc tgcgaccaac caacactgca cgttggcctg
     7561 ctggcaaagt aaagttcagc ggtccggcca gcgttttacc ttccggcgac gtgataaaca
     7621 gatcctcggc ctcaatggtc agcggatcgg tcaatgctaa ttccgcctca ccgcgctgcg
     7681 gatgggcgag cggggtttcc ataaacgttt tcagactatc agctgcacca acagcctggg
     7741 ctttagcatg ataaaacgta ccgagatcgc gtaatggctg gaaaaactct ggcgcaagga
     7801 tcagggccag aaaacccgca gccagcgtca cgccagtatc gtagtgacca aaatccagct
     7861 cgccgagata ggaaaaacca aagtagaccg ccaccagagc aattgacagc gaggtaaaaa
     7921 attcgagaat gccggaggat aaaaacgcca gtcgtagcac ttccattgtc cgttggcgga
     7981 aatcttccga agcagaacga atactttcaa tttcagcttc accacgacca aaaatacgca
     8041 atgtttccat gccgcgcagg cgatcgagga aatgcccact taagcgagca agagcgagaa
     8101 agttacgtcg gttagcatcg gcagccccca tcccaaccag cgccataaac aacggaatta
     8161 acggtgcagt gcccagcaga atgagcgccg cagcccagtt agaggggaag atcgccacca
     8221 caatcagcaa cggcaccgac actgccagcg ccatttgcgg caggtagcgt gcatagtaat
     8281 catgcatatc gtcaatttgc tcgagtacca gcgtcgccca gctccccgca ggtttaccct
     8341 gaatccacgc tggccctgct tgttgcagac ggtcgagaac ctgacggcgg atggcaaagc
     8401 ggatatgctg cccggcgtga taacccaccc gttcgcgtaa ccagaccacc catgctcgca
     8461 gtacaaaggt cagaaccagt aacgtaaagg gaagcagcag ggcttcacgg ggaatattct
     8521 ccataatcat atgttgcaga attcgcgcca tgaaccaggc ctgggcaatg atcaatatgc
     8581 cgcttacaaa gcccagcaga cgagaaatat tcagccaacg ttgggagatg acgctttgct
     8641 gttttaacca gcgggttaac tctttttgac gagatttatt cattgcacgc ttagcaggtg
     8701 agttatcaga attatttgca gagcaatgtt acaacgggga aaaaataaag gcgacccata
     8761 gtcgcaaggt gtcgccttct ttacttttgt tactgatttg taaaattatt ttgcgtcagc
     8821 taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg cagccagtac cggccgaagt
     8881 aatggcctgg cgataaatgt gatccatcac gtcgcctgcg gcaaagacgc caggaatgct
     8941 ggtctgggtg gcattaccat gaatacccga ctgtactttg atgtagccgt tttccagttc
     9001 cagctgccct tcgaaaatcg cagtattcgg gctgtgaccg atagcaacaa acagaccggc
     9061 aacgtcgagt gactcgatgt tgtcgctgtt ttgcgtatcg cgcagacgaa cgccagtgac
     9121 acccatttga tcgccggtca cttcttccag cgtacggttg gtgtgcagaa tgatgttgcc
     9181 gttctccact ttatccatca gacgcttaat aaggattttt tccgcgcgga aaccgtcacg
     9241 gcggtgaatc agatgcactt ccgaagcgat gttagacaga tacagcgcct cttcaaccgc
     9301 ggtattgccg ccgccgatga ccgcaacttt ctggttgcga tagaagaaac cgtcgcaggt
     9361 tgcacaagca gaaactccac ggcctttaaa ggcttcttca gagggcaggc cgagatagcg
     9421 tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg tattcgccgt tatcgccatt
     9481 cagacggaac ggacggtttt gcagatccac cttgttgata tgatcaaaaa tgatctcagt
     9541 ttcaaacttg gtggcatgtt cgtgcatgcg ctccattaat aacggaccgg tcagatcgtt
     9601 tggatcgcca ggccagtttt ccacttccgt ggtggtggtc agttggccgc ctttttccat
     9661 gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg tagacagcag cggtgtatcc
     9721 cgccgggcct gaacccagga taagcagttt actgtgtttg gtcgtgccca tgagatcccc
     9781 atagttgttg gcagacaatg ggcaggattg tagggaattt acagacgtaa aaaaagagta
     9841 tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga taagcaggtc aaattctccc
     9901 gtcattatca cctctgctac ttaaatttcc cgctttataa gccgattaaa tgatgaataa
     9961 acgcccctgt taatgaatat ctggcatgtt gtactaaaaa tcgatgtttt gctttgacaa
    10021 tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa acagtattct tatatgcgca
    10081 taaccatgca tgtaaatacc atgtttaccg tgctagtgaa atctacgtat ggcgtggaca
    10141 gacgccattc gtgatgtcga tagctgccac aaggcaacgg tcttctcacc gtagacccag
    10201 gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt gacgggtagc gactctgaac
    10261 agtgatgttt cagggtcaga caggagtagg gaaggaatac agagagacaa taataatggt
    10321 agatagcaag aagcgccctg gcaaagatct cgaccgtatc gatcgtaaca ttcttaatga
    10381 gttgcaaaag gatgggcgta tttctaacgt cgagctttct aaacgtgtgg gactttcccc
    10441 aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa gggtttattc agggctatac
    10501 ggcgctgctg aacccccatt atctggatgc atcacttctg gtattcgttg agattactct
    10561 gaatcgtggc gcaccggatg tgtttgaaca attcaatacc gctgtacaaa aacttgaaga
    10621 aattcaggag tgtcatttag tatccggtga tttcgactac ctgttgaaaa cacgcgtgcc
    10681 ggatatgtca gcctaccgta agttgctggg ggaaaccctg ctgcgtctgc ctggcgtcaa
    10741 tgacacacgg acatacgttg ttatggaaga agtcaagcag agtaatcgtc tggttattaa
    10801 gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt gattacactc ctgttaatcc
    10861 atacagcaac agtactgggg taacctggta ctgttgtccg ttttagcatc gggcaggaaa
    10921 agcctgtaac ctggagagcc tttcttgagc caggaataca ctgaagacaa agaagtcaca
    10981 ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt tgctgatcct tattgtcctg
    11041 tttgccgtct ggttgatggc tgccttacta agctttaacc cttcggaccc cagctggtcg
    11101 caaacggcct ggcatgaacc tatccataat ttaggtggga tgcccggtgc gtggttggca
    11161 gatacgctgt tctttatttt tggcgtgatg gcttacacca ttcccgtcat tattgtcggc
    11221 ggttgttggt ttgcctggcg tcatcagtcc agcgacgaat acattgatta ttttgccgtt
    11281 tcgctacgca tcattggcgt tttggcgctc atccttacct cctgtggtct ggcggcaatc
    11341 aacgctgacg atatctggta ttttgcctcc ggtggcgtca ttggcagctt actaagcact
    11401 acgctacaac cactgctaca cagtagcggg ggaactattg cgctgctctg cgtttgggca
    11461 gcgggcctga cgttgttcac cggttggtca tgggtgacca ttgctgaaaa actcggcggc
    11521 tggattttaa acattctcac cttcgccagt aatcgtaccc gtcgcgatga tacctgggtc
    11581 gatgaagatg aatatgaaga tgacgaagag tatgaagatg aaaaccacgg caaacagcat
    11641 gaatcacgcc gtgcccgtat tcttcgcggc gcgctagcgc gtcgtaaacg gttggcggaa
    11701 aaattcatta atccgatggg gcggcaaaca gatgctgcgc tgttctccgg taagcggatg
    11761 gatgatgaag aagatattac ctacactgca cgcggtgtgg ctgccgaccc tgacgacgtc
    11821 ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg aatacgatcc attattaaat
    11881 ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg ctaccacggc gacacaaagc
    11941 tgggctgcgc cggttgaacc tgtgactcag acgccgcctg ttgcctctgt tgatgttcca
    12001 ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc cacaaacggg agagccggtt
    12061 attgctcctg caccggaagg ttacccacag cagtcacaat atgcgcagcc tgcagtgcaa
    12121 tataatgagc cgctgcaaca accagtacag ccgcagcagc cgtattatgc acctgcagct
    12181 gaacaacctg cgcaacaacc ttactacgct cctgcggcgg aacaacctgt tcagcaaccg
    12241 tattacgcaa ctgcgccgga acagcctgcg caacagccgt attatgcccc tgcgccagaa
    12301 caaccggtgg caggtaacgc ctggcaagcc gaagagcagc aatccacttt tgctccacag
    12361 tctacatacc agactgagca aacttatcag cagccagccg ctcaggagcc gttgtaccaa
    12421 cagccgcaac ctgttgaaca gcagcctgtt gtggagcctg aacccgttgt agaagagaca
    12481 aaacccgcgc gtccgccgct ttactacttt gaagaagtgg aagagaagcg agcccgtgaa
    12541 cgtgaacaac ttgcggcctg gtatcaaccg attccagaac cggttaaaga accagaaccg
    12601 atcaaatctt cgctgaaagc accttctgtt gcagcagtac ctccagtaga agccgctgcc
    12661 gctgtttctc cgctggcatc tggcgtgaaa aaagcgacac tggcgacggg ggctgccgca
    12721 accgttgccg cgccagtctt cagtctggca aatagcggtg gaccgcgtcc gcaggtcaaa
    12781 gaggggattg gcccgcagtt gccacgaccg aaacgtatcc gcgtgccaac tcgtcgtgaa
    12841 ttggcgtctt acggtattaa gctgccctca cagcgtgcgg cggaagaaaa agcccgtgaa
    12901 gcccagcgca atcagtacga ttctggcgat cagtacaacg atgatgaaat cgatgcgatg
    12961 cagcaggatg aactggcacg tcagttcgcc cagacacagc agcaacgcta tggcgaacag
    13021 tatcaacatg atgtgcccgt aaacgcagaa gatgcagatg ctgcggcaga ggctgaactg
    13081 gctcgtcagt ttgcgcaaac tcaacaacaa cgttattccg gcgaacaacc ggctggggcg
    13141 aatccgttct cgctggatga ttttgaattt tcgccaatga aagcgttgct ggatgatggt
    13201 ccacacgaac cgttgtttac gccaattgtt gaacctgtac agcagccgca acaaccggtt
    13261 gcaccgcagc cgcagtatca gcagccgcaa cagccggttg caccgcagca gcagtatcag
    13321 cagccacaac agccggttgc gccacagcag caatatcagc agccgcaaca gccggttgca
    13381 ccgcagccgc agtatcaaca gccgcaacaa ccagttgcgc cgcagccgca gtatcaacag
    13441 ccgcaacaac cggttgcacc gcagccgcag tatcagcagc cacaacagcc agttgcgcca
    13501 cagccgcagg ataccctgct tcatccgctg ttgatgcgta atggcgacag ccgtccgttg
    13561 cacaaaccga cgacgccgct gccttctctg gatttgctga caccgccgcc gagcgaagtg
    13621 gagccggtag atacctttgc gcttgaacaa atggcgcgtc tggtggaagc gcgtctggct
    13681 gatttccgta ttaaagccga tgtcgtcaat tactctccgg ggccagttat cactcgcttt
    13741 gaacttaacc tggctcctgg cgtaaaagcg gcgcgcattt ctaacttgtc acgggacctt
    13801 gcccgttcac tttcgacggt ggcggtgcgt gtcgttgaag ttattcctgg caaaccctat
    13861 gtaggtctgg agttaccaaa taaaaaacga caaaccgttt atctgcgcga agttttggat
    13921 aacgccaaat tccgcgataa tccgtcgcca ttaaccgtgg tgctgggtaa agatatcgcc
    13981 ggtgagccgg tggttgccga tctggcgaaa atgccgcact tgttggttgc ggggactacc
    14041 ggttccggta aatctgtcgg tgtgaacgcg atgatcctga gcatgcttta taaagcacag
    14101 ccagaagatg tgcgtttcat catgatcgac ccgaaaatgc tggagctttc ggtttatgaa
    14161 ggcattccgc acctgttaac ggaagtcgtt actgatatga aagatgccgc caacgcgctg
    14221 cgctggtgtg ttaacgagat ggagcgtcgg tataaactga tgtctgcgct gggtgtgcgt
    14281 aatctggcgg gttataacga aaaaattgct gaagccgatc gcatgatgcg tccgattcca
    14341 gacccgtact ggaagccggg tgacagtatg gatgcccagc atccggtgct gaaaaaagaa
    14401 ccatacattg tcgtgttggt tgacgaattt gccgacctga tgatgacggt aggtaaaaaa
    14461 gtggaagagc tgatagcacg tctggcgcaa aaagcccgtg ccgcgggtat ccacctcgta
    14521 ctggcaactc agcgtccatc ggttgatgtt attactggtc tgattaaagc gaatattccg
    14581 acccgtatcg cctttaccgt atccagtaag attgactcac gtaccattct tgatcaggct
    14641 ggcgcggaat cactgctggg tatgggggat atgctctact ctgggccgaa ctccacgttg
    14701 ccggtacgtg tccatggtgc ttttgttcgc gatcaggaag ttcatgccgt ggtgcaggac
    14761 tggaaagcgc gtggtcgccc acagtatgtt gatggcatca cctccgacag cgaaagcgaa
    14821 ggtggtgcgg gtggtttcga tggcgctgaa gaactggatc cgttgttcga tcaggcggtg
    14881 cagtttgtca ctgaaaaacg caaagcgtca atttctggcg tacaacgtca gttccgcatt
    14941 ggttataacc gtgcagcgcg tattatcgaa cagatggaag cgcaggggat tgtcagcgaa
    15001 caggggcaca acggtaatcg tgaagtgctg gccccaccgc cgtttgacta attaatgcat
    15061 tgccggataa ggcgcggtag cgtcgcatcc ggcactctat caactgaaaa ttcagtattt
    15121 tcttctttcc tcaagctgat tattagcctg gaatagagag tagagggaac tcccgatcgg
    15181 gagtgacgta atttgaggaa taatgatgaa aaaaattgcc atcacctgtg cattactctc
    15241 aagcttagta gcaagcagcg tttgggctga tgccgcaagc gatctgaaaa gccgcctgga
    15301 taaagtcagc agcttccacg ccagcttcac acaaaaagtg actgacggta gcggcgcggc
    15361 ggtgcaggaa ggtcagggcg atctgtgggt gaaacgtcca aacttattca actggcatat
    15421 gacacaacct gatgaaagca ttctggtttc tgacggtaaa acactgtggt tctataaccc
    15481 gttcgttgag caagctacgg caacctggct gaaagatgcc accggtaata cgccgtttat
    15541 gctgattgcc cgcaaccagt ccagcgactg gcagcagtac aatatcaaac agaatggcga
    15601 tgactttgtc ctgacgccga aagccagcaa tggcaatctg aagcagttca ccattaacgt
    15661 gggacgtgat ggcacaatcc atcagtttag cgcggtggag caggacgatc agcgcagcag
    15721 ttatcaactg aaatcccagc aaaatggggc tgtggatgca gcgaaattta ccttcacccc
    15781 gccgcaaggc gtcacggtag atgatcaacg taagtagagg cacctgagtg agcaatctgt
    15841 cgctcgattt ttcggataat acttttcaac ctctggccgc gcgtatgcgg ccagaaaatt
    15901 tagcacagta tatcggtcag caacatttgc tggctgcggg gaagccgttg ccgcgcgcta
    15961 tcgaagccgg gcatttacat tctatgatcc tctgggggcc accgggtacc ggcaaaacaa
    16021 ccctcgctga agtgattgcc cgctatgcga acgctgatgt ggaacgtatt tctgccgtca
    16081 cctctggtgt gaaagagatt cgcgaggcga tcgagcgcgc ccggcaaaac cgcaatgcag
    16141 gtcgccgcac tattcttttt gttgacgaag ttcaccgttt caacaaaagc cagcaggatg
    16201 catttctgcc acatattgaa gacggcacca tcacttttat tggcgcaacc actgaaaacc
    16261 cgtcgtttga gcttaattcg gcactgcttt cccgtgcccg tgtttatctg ttgaaatccc
    16321 tgagtacaga ggatattgag caagtactaa ctcaggcgat ggaagacaaa acccgtggct
    16381 atggtggtca ggatattatt ctgccagatg aaacacgacg cgccattgct gaactggtga
    16441 atggcgacgc gcgccgggcg ttaaatacgc tggaaatgat ggcggatatg gccgaagtcg
    16501 atgatagcgg taagcgggtc ctgaagcctg aattactgac cgaaatcgcc ggtgaacgta
    16561 gcgcccgctt tgataacaaa ggcgatcgct tttacgatct gatttccgca ctgcataagt
    16621 cggtacgtgg tagcgcaccc gatgcggcgc tgtactggta tgcgcgaatt attaccgctg
    16681 gtggcgatcc gttatatgtc gcgcgtcgct gtctggcgat tgcgtctgaa gacgtcggta
    16741 atgccgatcc acgggcgatg caggtggcaa ttgcggcctg ggattgcttt actcgcgttg
    16801 gcccggcgga aggtgaacgc gccattgctc aggcgattgt ttacctggcc tgcgcgccaa
    16861 aaagcaacgc tgtctacact gcgtttaaag ccgcgctggc cgatgctcgc gaacgcccgg
    16921 attatgacgt gccggttcat ttgcgtaatg cgccgacgaa attaatgaag gaaatgggct
    16981 acgggcagga atatcgttac gctcatgatg aagcaaacgc ttatgctgcc ggtgaggttt
    17041 acttcccgcc ggaaatagca caaacacgct attatttccc gacaaacagg ggccttgaag
    17101 gcaagattgg cgaaaagctc gcctggctgg ctgaacagga tcaaaatagc cccataaaac
    17161 gctaccgtta atgttatcgt tgcggtaatg ttgttactgt atccctgtgg tcgcaggctg
    17221 tggccacatc tcccatttaa ttcgataagc acaggataag catgctcgat cccaatctgc
    17281 tgcgtaatga gccagacgca gtcgctgaaa aactggcacg ccggggcttt aagctggatg
    17341 tagataagct gggcgctctt gaagagcgtc gtaaagtatt gcaggtcaaa acggaaaacc
    17401 tgcaagcgga gcgtaactcc cgatcgaaat ccattggcca ggcgaaagcg cgcggggaag
    17461 atatcgagcc tttacgtctg gaagtgaaca aactgggcga agagctggat gcagcaaaag
    17521 ccgagctgga tgctttacag gctgaaattc gcgatatcgc gctgaccatc cctaacctgc
    17581 ctgcagatga agtgccggta ggtaaagacg aaaatgacaa cgttgaagtc agccgctggg
    17641 gtaccccgcg tgagtttgac tttgaagttc gtgaccacgt gacgctgggt gaaatgcact
    17701 ctggcctcga ctttgcagct gcagttaagc tgactggttc ccgctttgtg gtaatgaaag
    17761 ggcagattgc tcgcatgcac cgcgcactgt cgcagtttat gctggatctg cataccgaac
    17821 agcatggcta cagtgagaac tatgttccgt acctggttaa ccaggacacg ctgtacggta
    17881 cgggtcagct gccgaaattt gctggcgatc tgttccatac tcgtccgctg gaagaagaag
    17941 cagacaccag taactatgcg ctgatcccaa cggcagaagt tccgctgact aacctggtgc
    18001 gcggtgaaat catcgatgaa gatgatctgc caattaagat gaccgcccac accccatgct
    18061 tccgttctga agccggttca tatggtcgtg acacccgtgg tctgatccgt atgcaccagt
    18121 tcgacaaagt tgaaatggtg cagatcgtgc gcccggaaga ctcaatggcg gcgctggaag
    18181 agatgactgg tcatgcagaa aaagtcctgc agttgctggg cctgccgtac cgtaaaatca
    18241 tcctttgcac tggcgacatg ggctttggcg cttgcaaaac ttacgacctg gaagtatgga
    18301 tcccggcaca gaacacctac cgcgagatct cttcctgctc caacgtttgg gatttccagg
    18361 cacgtcgtat gcaggcacgt tgtcgcagca aatcggacaa gaaaacccgt ctggttcata
    18421 ccctgaacgg ttctggtctg gctgttggtc gtacgctggt tgcggttatg gaaaactatc
    18481 agcaggctga tggtcgtatt gaagtaccag aagttctgcg tccgtatatg aacggactgg
    18541 aatatattgg ctaataccta atttttctga atctaaaaag cgcctgcggg cggtttttgc
    18601 atgagattaa ttaactgaaa ataaaaggga tttattttca ggtgtccata taatgtccat
    18661 ctttgaaaca aaaaagcccg cagtttttac gctacgggct ttgtttttct ttgtgacttg
    18721 cgtgtgaggg tgctactttc gaccgaacct ttggcaaccg gatagcgggg gctttcaccc
    18781 ccgctgcggt ttcttacggt ttacactgca agaacgccgc aaattctgac cacatgatgt
    18841 taagtctgaa ctcacacggc gaacggtgta tcatccagac tgtcaacaac atggttaagc
    18901 ataaaatgct cacgatgatc gttttttgcg gcatagcgct tgctcctttt tcaaagaggc
    18961 gctaaccttc cacttgcgaa ggttagatgg ttagggcctc gggttaaaca aatgtttgac
    19021 tcggggcctt ttcacatccg gccttcaggt aatccttcca gccgtcagcc gaaaggcacc
    19081 cgcgcgtaat gtactggttt taaagtcata ccgcaatcct ccagttgtag cgttaactga
    19141 ctgcgtccct gatgggattt gcgcattgtc tcccgtggaa tatctttatc gtggaagatc
    19201 aactgaggct ggccaataat tctgtgcaca ctttccattg ttgaaaatgt ggcgctgcac
    19261 tcaaggttat tgcactgata gtatttgtag cggatggtgc cggtatcatt ggcggcttta
    19321 ctggtacgga tccgggcaga tgcgccgcag aatggacaat gaaacatcga tataccccct
    19381 ggctgaactg gggttgaact cgtcgctatt ctaaccattg ctttcctttt cggctatcca
    19441 ctcgggtatt cttgcctcca gttcaagcct tgtggtgaag ccgcttccgt cgatattgtg
    19501 ttccactttt gcaatgagcc agtcctgatt gtctatctcg gttttaaagc cggttaccgt
    19561 gccatgcatt tcagggtaaa gctccgctct gccccgggcc agcgtgatgg agaattcagc
    19621 cgcaccgcgc tgtaattgct gccacctggc ggcagctgcg cgctgtgcag caatttcatt
    19681 tgggtaggtt tttctcagaa cgaacacgtt accttctgcc ccctccatgt agctgccttc
    19741 ccggctgctg ctcttttcag cgtgagtttt tcgttttttt atgttgacgg ctttcttttt
    19801 gccatgattc aaatccagcc agtacgcctt cacccctgtg tatgcctggc gatcggctat
    19861 tctgaactgg tgactgtctc cgtcactgcg tgccagtgca aatgagggga gtgggcgccc
    19921 gtcagctgtc acgccgccac ccggcagaat gaacagcaga cagccatttt tgacggtagt
    19981 gatggctccc agcatatcag ccatgcgggt aaggaatgat atgtcgcttt cttccgtctg
    20041 gtcggcatga tcgatttcaa tatccataag catatcgctt atctgggctt tcagtccgta
    20101 acggtgggca atagcggaaa ccacgcgttc aacagtcacg tcgtgccagg atacttcccg
    20161 tttgacgtta aattcctccc tgaagtctgc gctgtttgca ctcagtgtca gccgatccgg
    20221 aggtccttca tgagcgacct catcaacaat gtaggtgcct ttatcaacca gaggctctcc
    20281 cttccatccc agcatgactc tgatgtgtgt tccccgcccg gggagagcca gttgcccgtc
    20341 ggcatcatcc agcacaagag tgaccctgtc tgcttcaaac ccccggttgt ctgtcatgga
    20401 aagcgatatc acccggttat tcagttcctg aagtgtgttt ttatcctgct ccctgtccag
    20461 tgaaacgctg aaatcaggca ttttcaccag ttctttcagg ggggaataaa ccgtgttacc
    20521 gttcagcaat accataattc ctccgttttt cccgccacag ttgcatacca gaaacaccac
    20581 cggaatgagt ttgggttgta tgtgtattgc gagaaaccgc agttcgtgaa aaagctcccc
    20641 gtgttcgtaa agatctctgc tgaactcaca cgaatgcagg gtgaataata tggctgaaac
    20701 ccgtttccat ggcgtgcggg tccgggagaa tgcggatctg gtcacggcca ttaatgatat
    20761 tgactccagt gtgattggta tcgtggctgt tgctgatgat gctgatgcaa aaacatttcc
    20821 gctggataag ccggtgctcc tgacgcgtgt catggatgtg ctgggtaagg ccggaaaaac
    20881 cggcacgctg tataaaagtc tgaaagctat tgccgatcag gtcagcacca gagttgttgt
    20941 ggtgcgggtt gctgctgcga aggaggggga tggtcagccg acacaaacgc agcttgttat
    21001 tggtgaggtc aaaaatgacg gaacctatac aggcatgcag gcgctcctgg tggctgaaca
    21061 gaatgaggct gttggtgttc gtcccaggat cctggccgtg ccatcgcttg acaccaaaga
    21121 ggtggtggcg gcactggtaa ccgtcgcgaa aaagctgcgt gcctttgtgt atgcgggctg
    21181 tcccggctgt aataccgtgc aggaggccgt tacctggcgc gccgatcttg ctcaccgtga
    21241 agttatgctg atctggcctg attttatcgc gtacaaccct aacagtggcc agaatgaggc
    21301 ttttcctgca tctgcgtatg cgtgcgggtt gcgtgcgaag attgaccacg agcagggctg
    21361 gcataaatcc ctgtccaatg tggcagtgaa taatgtcctg ggtatttctg tgccggtgtt
    21421 ctggtcgctc caggcagagg acagtgacgc ggaaaccctc aatgacaagg agatcaccac
    21481 actcattaaa cgtaacggct tccgtttctg gggtaaccgg actctcgaca ccaccaccta
    21541 cacctttgag acctacacgc gtacggcgca gtcgctggcg gataccatcg ccgaatcgca
    21601 gtttgaaacc gtggacaagc cgctgacgcc gtcgaacgtg aaggatgtgt taagcgatat
    21661 ccgggcaaaa ctgagtgccc ttgtgacggc gggtaaactt atcggggcag agtgctggta
    21721 tgacgtggtg gataacggca caacggatct gcgtcagggg gttgtgcgca ttcgctacaa
    21781 gtatacgccg gtgccaccgc ttgaagatct tgagcttcac cagacgttta ccgatgaatt
    21841 ctttgcgggt gcatttgcat cactgggagg tgagtaatgg cggtaccaaa caaacttcgc
    21901 gttttcacgg tgtacatcga tggcgaaaac aagctggggc gggtgacgtc cttcacgccg
    21961 cctaaactga cccgaaaaac ggagtccttc cgtggtgccg ggatgcccgg cagtgtttcc
    22021 gtcgactttg gtcttgatga tggtgcactg gatctctctt ttgcggtagg cggcgcagat
    22081 gcccaccttt tctcccgtta tgccgggtca cttgatgaag tccgtcttcg cttttccggt
    22141 gagtactatt cggatgcgga tatcagatat gtggatattg aggtccgcgg gcgcatcact
    22201 gaaatcgata tgggggaggc gaagcagggt gaagacactg agcacagtta ttcgatgaaa
    22261 aacacctggt acagactgag tgtggacgat cgggaaacaa tcgaatttga tgtgctgaat
    22321 tttatctggc gcattgacgg taaggatgtg ctgccggacc gtacccgctc aatgctgggt
    22381 ctctgatgat ggtttaactg agggcggcgg ggatcctgcc gtggagactg aaaatggata
    22441 aagaaacgga aatggccact gacactgccg tcagtaacgg ggaggcttcc gcggtggcgg
    22501 ttgccgctac ggctgcacct aagaacgtgg tgaagctgac ggtgcctgtt gtgcgcggag
    22561 atgtgtcgta cagtgaaatt aaaatcggac cgctggcgaa tcaggccgga tcgctgcgtg
    22621 ggctctcgct gcttgcgctc tacaacatga actgtgatgc tatggtcacc tggcttgcac
    22681 gtgtgacaga gccaaaactt aaggaagccg aaatgtatgc tatgagcgtg gtggatttta
    22741 cggcgctgag tacggcggcg gttaattttt tcgcaccctc ggggtcgaat gcaccggcac
    22801 cggagaacac ggcagagccg taacctgtct ttgctttgac tgcattgaag atcccgttgc
    22861 ggatattgct gttattttta actggccgcc cgcagagata tttgcaatga cgcctgcaga
    22921 ggtggtcgcc tggcgtgagc gggcggctgc cagaagcgga agtcacaatc atgatgaata
    22981 atctgcatat cagggttgct ctggcggcgg cagacaccct gacgcaacct ctcaggcgcg
    23041 cccgcgaaac cgtgggcgcg ctttctgatt ccctccgtga aactcaggac tccatccgct
    23101 cgcttgaacg gcagagtgaa gcctttgctg cggcacagac taaatttgac gaagccaccc
    23161 ggaaaattgc cgctgttcag cagcaactga accgtctgca gcaggcggag cgtgagggta
    23221 ccgtgctcag tgaacggcag cgtgaaatga tggcttcgct gaccacccgg cttgaacgtc
    23281 tgaaccgggt gagggatgta catcagcgac gcctggagga ttcacgccgg gagctggaga
    23341 gacacggtat tgccgcagag ggcagcagtc agaccataca ggcagccatt cgccggacgg
    23401 cagagtataa cgaagcgctg gcgcgacagc ggcaggcact cgcacaggtg acccgtgcac
    23461 agcaacgcta tgaacaggcg caggagcgac tcgggcggat acgtaatgcc ggtgcggcgg
    23521 cactggctgc cggtgcagca ggactttacg ctggcgggcg tgccgttgcg ccggtgatgg
    23581 atctccagcg tcatggtgcc gtcattgcgg ctcaggcagg agagggcgcg tcagatgccg
    23641 tggggtatac cggggttata aaccacatgg ttcataccgg tatcacaccg gatgcacaac
    23701 tggcggctga agctgtgggt gctgtccgct ccacgctggg tgggctgggg gcggttagtg
    23761 agaatgaact gacccgaatc acccgaaaaa tgctggatat gcaggccgtg atggggggcg
    23821 atatcccgca gcacattcag gcggctgcca tcatgatcaa aaacggtctt gcacgcagca
    23881 gtgacgaggc ggttgatctg atggtggcgg gcatgcagcg catggggccg gttatgcgcg
    23941 atgagctgcc tgagatcctt cacgaatatt cgacccattt tcgtaacatg gggctgacag
    24001 ggtcggaaac catgacgctc cttgcggata tggcgcagca ggggaagttt gcgctggata
    24061 aaaccggcga tgccatcaag gagttcagta ttcgcggctc cgatatgtca aaggcgagtg
    24121 ctgaagcgta tgcgcttatc gggctggatg cagccaggat gtcatcggct gtagccagtg
    24181 gtggaacggc tgcacgggct gcgctgcaga aaacagcctc gggtctgttg cagatcgtcg
    24241 atccggctga gcgggccaat gcggccatcg cactttttgg tacaccggtt gaagatctgt
    24301 ctgtcgatca aatccctacc tttcttggcg cgctggcgcg ggtgtctgac cgtatggctg
    24361 acgtgtctgg tgtggcggac cggatggggc aaaccctgcg tgacaatctg agtggtgatg
    24421 tggcgcggct taccgggacg ttcagcgcgc tgagaagtgg gattatcggg gagattacgg
    24481 acgatctgcg tgggcttgtc cggatggcca cagcgtgggg tgagcgtctg caggcgtggg
    24541 tgaatgcgca tccggaactg gtgcgaatga ttgcgctgac gacgggagcg gtgctggcgt
    24601 taaccacagc gatgggggcg ttgtccgttg cggcctggat ggttgtcgca ccgttactga
    24661 aactgcgtct ggggcttgcc ctgcttcgcg cgatcccggc cggactctcc gttgccggta
    24721 cgctgtttgg cggactgacg ggtgtgattt cagccgtggg ggctgcgctt ggggcgctga
    24781 cctggccggt tgtggtggcg gtgggagccc tggtcggtgg tgctctgctg gtctggcgct
    24841 actgggatcg ggtcagggcg tttgtgagcg gatttattga aggggtacag tcagcactgg
    24901 cgcccttcat ggcgacagcc tcccgttttg cgccgctgct ggatctggtt tcaggggcac
    24961 tgtcccgtat tctgggatgg tttaaatccc tgttaacgcc ggtctcctcc acgcgtgatt
    25021 tgcttgagca gtgcacctct gccgggcaga tgttcgggag cactctggcc ggtgcgttag
    25081 ccacggtttt ttcgcccctg aaagctctga ttgacggtct ggcatgggtg ctggaaaaac
    25141 tcggtgttct gcccggtgaa gctgagcggg ttaagcgaaa actggatgag gcccgtaaaa
    25201 cgccggtggc gtgggagtgg gatccggaac agaaaaaaat ggttcagaag gcgtggcact
    25261 ggtcgcctcc tggtggggcc agtgtgcctt ccggcgcctc cggtacacag cctccgccgg
    25321 tggcgctccc tgcggggata gcgccggacg gtaaccgtaa cgttctccgt cacctgaaag
    25381 gcattgaggg gaataccggt ggtctgctgg ctgaaaaccg taaacgtatc ggacccggag
    25441 atattgtgtt taaacatctt ccacgggcgc tggcggtccg ggggcagtgg catgaacctg
    25501 tcgttgcgcc gtcttccggc ggaatgcgcg cgctgccaca ggaggagatt caggtgcagc
    25561 aatggccgac ggcacgcccg gcgccacgaa tgcaccagca ggaaaccttc gccagcgcct
    25621 ttcaggggga gatacatgtg catcttcacg gggtgagttc gcataatccc cgggaactgg
    25681 cgcgcatggt tggtgacgct gtcagggcgg aaatgaacag aatggcacgt gccggaaccg
    25741 gcagcttccg cgatcgcgac tgaggtgaaa aagatgatga tgatttatgg gatgtttgtg
    25801 tttcagttaa gtaccctgcc gcatcagcag gtggaacaga gtaaacgctg gcgacacgtc
    25861 agtaatgaac gggttaaccg ttcatcctcc tggcagtata ttggctctgg tgaagatcac
    25921 atcacgatgt ccggtgtgct ctgtccggaa atcaccggcg gggaggtgtc actgacagcc
    25981 ctgaacaccc tggcctacac aggattaccc tggccactga ttgatggtgt cggacagatt
    26041 tacgggatgt atgtcattac ggaaatcagg accacccgtc aggagctgga tcgctatggc
    26101 atggcccgca aaattgagtt cactgtcagc tttcagcgat gcaatgaaga tctgcgtgag
    26161 cgtcttcagt cgggctcggt gggtgatctg atggcaggat taaaaaacag cgcgtcgtcg
    26221 gccctgtcgt cggcgtcctc tgcgctgtct ggtctcatgt tgtgaaggtc actgttcagg
    26281 cttcggcttc ttttccccag ccaaccttat agtccagaat cgccgccgta tcgttcaggg
    26341 cggcgatctc ttttttcatg ttacgctggc gaatgtgaat ttccatccct ttggcaaaca
    26401 gggcttttgc ggctgcatcg ctgagggcaa tcagctcatc agcagaaaca ggaacatcgt
    26461 tgttctgtgc atcagtccag aaaaaatcca gcggcaggat ccccgctttt gcggcagcaa
    26521 cggaaggctc aaggcgggcc tgcgttgact tcccgtagtc ccatttccgg ccgttatgtt
    26581 caaaggtgta actcgccgct tccatcgcat tacgccaggt gttgatttcg tcgtttttca
    26641 ggcgtctggc gttttccctg tcagtgtcgt tttcctggta ttcagataca gggccgtgat
    26701 ttcctgcttt cagttcgccc cagagttttt tgccgtgtgg cgagttgtca tattcggttg
    26761 ccgtataggg cagccagctc tcctcccctt caaactggat ctcacagtcg atagtgccgt
    26821 cacggttata agcggcattt cttgctgatt tgattgtgtg catgagtctc tccgtcaggc
    26881 aatgcgctca aatattccga ttcctgcgcc tgcataaatg tcagcgcagg cccgccacgt
    26941 gcccggtggc ctgcggtttg tgttccagtt ttgcatggat tcacgggggc cgaagttgcc
    27001 ttcggaatga gtcaggctta tcagtctgag tttactgccc gggcgggtgt ttccggccgt
    27061 tatatcaatt ttggctggcc actccccttc ggcatccgtt gccgccagaa taaagctgcc
    27121 cacatcccag agtgtccgct gtgtttttgc ctgtgcagcc tccagtgctt gccgtgcttt
    27181 ctgcccggcg ttttctgctg ccgtcatggc cgtcttcaca gcttttggtg tggcagccag
    27241 cacttcactg tcactgtccg ttgcgctgct tagctgaaca atacctcgct ctgtcaggga
    27301 ggcatcgccg gtctgcgtga tggggagtat ggcaacctcc ttccattcag accacggccc
    27361 gttgccggtg aagttacctg tcactgtccg gataaatttc cgtggtggat tccaggcggt
    27421 gtattcctgc tggcagaatc cccatgcgcc catcctgatg gataatgatc cggctgatgt
    27481 taccgggtag tgatgtgctg tagtggcgcc gttgttgttg ggctgatagt agtcaccatg
    27541 atgttcaggt ctccccagtg tattcagatc cacggcgagg ggggctgttg gtcttgttac
    27601 tgtcagcgcc ttaagtgctt ttattgcttt gggtgtggct gcccgggttt catcatcact
    27661 gtcggtttca ctactgagct gagtaaatcc tttactggtg gttgtggcat ccgggtgttt
    27721 accgttttgt tcatgctgct ttatcatttc tgccacgcca gcctgtgttg ccatggtggt
    27781 tgatgagtcg gctgtcagat ttacagaggc ttcgttgctc agcagaatca ccatgcggaa
    27841 tacctgttct ctgcctgagc cctcatcaag ctgcggtttg taaccttccg caacattacc
    27901 cacggcgatg agtgtgcctt tgctgtcaaa cagaccgatt tcgcggatcc agaatcctcc
    27961 ttcattcgca ggaatgatca cttcggcggt taaaaaactg gtgttttccg cgtcacggct
    28021 gaccctgctg acctcgcccc gccagacctc tttaacgaga gcggtttgcg tggccagtgg
    28081 ttctgtgggt ttaccgttac cgtctccgac ggcgatatgt gtcagcgtga tcttttctcc
    28141 atccggtaac accgcagctg cgagggctgc tgtgccggca tttgtgataa tggttttgta
    28201 ttttgcggcc atcattactc cttgtccggt ctgatggtta ctgtgatgcc ttcataggtg
    28261 actccacccg ggtggatgtc accactgact gacagcgggc ttgtcacttc tgctgccgga
    28321 tataccacga tggtttcccc ggtcagcgtg gccccggtta tgcgggatac accccgtgca
    28381 acgtaggtca ttttcatggc ggcaaggtgt cggcttaccg ggcgaacggt ggtaatcatt
    28441 cgctccatct cctgccgggt ttcctctgtc agtccgcgct ccgatatgtc aatatccagc
    28501 ctgaatgacc ccggcggatc gttgttctcc caccattctg taatactcag tatcgcgccg
    28561 aagggctcca cagcccgacg catggcgctg atggtgcctt tgtggcggtg aatatgccac
    28621 gagtcacgga tgatctgacg acgtgcctct tcactccatg tttcatccca gcggtcaacg
    28681 gaaagcgccc aggcaagcca tggcagcaga tgggctgggc aggtgtcggg gttccagagg
    28741 gtgttgatat ggatcggcag ctctgacagg cgctgagcgc cctgttccga atgatgcagg
    28801 aaggagggcg atgatggcgg cagcagtcgg ttattcatcg gtgccacctg cagagatgtt
    28861 ccgggatgtg cagcgggctg actgggtgtt actgatcacc acattctgcg ctggtttctt
    28921 cagctcgact cgctgaacgc ccggtacgtg tagcgttgcc ataatggcag agcgggccac
    28981 atcacgccct atggcattct gagtcttcag ccatgtgtca agtgcatctc tggcggcggc
    29041 aagaatgggt tcagattccg ggccaggata aaaatagagt acggcctcaa tatcgtaact
    29101 gacgatggtc gcactctgta ccgtgagtct gtcggccagg gggcgcacag tttcatcatt
    29161 gagagcccgg ctgacggtcg cgattaattc cggtgacgca gtgccgtctc cttccgttga
    29221 gagaatggag acaatgaccg tcgcaggtga cggactggtt gcccgtgcat cggcaacaag
    29281 gccgctggca ctgcgggcaa aatattcata tgcagcagag gggccggcga cgctgaaacc
    29341 ttcaaatccg gattgtgccc gcagacggaa cgcgctgtcg ctctccatta tggcagcttc
    29401 tgtatcagta gcaggtttca gttcaaggcg tttcgcattc acgatagcgg cgagattatc
    29461 caggtctgtg gatacggcat ggctgagcat gcaccccgcc gctgcatcgt tgatccgctg
    29521 acggagcagc atttcgcgaa aggcgatggc ctgagcaatg atgttgagtg gctctgattc
    29581 cagcgcaatg gctgtgctga cggcactgcg catgttctca gggaaggcgt caatcataaa
    29641 ggccttcacg tctttcagta tgctctcata atccagtgat tcaatgatct gcggacgtgg
    29701 taactgagac aggtcaattg tcggcattac tccccctcag cggaatgctc atctgtacgg
    29761 gctgcattgt gcgggtgagc acgcctgaga tggccagcag agcgcgtccg tcaggcagcc
    29821 aggtgatatc cacccggtta atggcgatcc ggggctccca ctgtgcgagc gccataacca
    29881 ccgcgctcat gcactgcaga cgcgtcacgt cgttttgtgg cgcatcgagc agttcaggca
    29941 acaggctgcc atactctctt cgcatgatgc gactgccgcg cggggtggtc agaatattgt
    30001 ttacagagtg gcggaggtgt tctgtgtcac tgatgcatcc gctaccatgc gggttcatgc
    30061 ctgtgcatac cggtgtcatt tcggcccccc tgtcatgctt ccgccggact gaacaccgtt
    30121 atgaacgtgc gtatgcacgg taacaccatt tgagcgtaag tttccgttac tgtgtgtgat
    30181 atcccctttc agggtgccac cgtcagtcag ttcaaacgtc ctggccttca gatgttttgt
    30241 gcactctacg gccggtgtgt ccagggtaat acgtgtttcc gcagagatct ctgcggtttt
    30301 gattcctttc accgtcagcg cgcctttttc tgcgtcatag cgtatctcgg caccgtcagg
    30361 tgcaaggagt atcacttcat tctgttttgt gccgggcgcc gggtgttctt cgctgtagag
    30421 actgccagcc acaacggcca tttcaggact gccgcccggg cagagcaaca gcacctgttc
    30481 accgggggag gggggcagcc agattttaaa cgccccggcg cgtgtgcagt tccagcggag
    30541 ccagtccgtt gtcagtccgc cgctggtaac gcgaatgcgc cagtttttca gatccacctc
    30601 cgaaacgacg ccggtgcgga ccatgttttc cagcagtcgc atgatttcag cattcaacgg
    30661 gcgtttctcc acaggttgtt gataatgatt tccgtcactt tctgtctgtc ggcttcactg
    30721 aatcccagca gttcgcgttg cggatagcgg acggcattac cggcagagac cagtgacact
    30781 tcgccgaact gatgaacgcg ggcaatgcgg gctgcgcggc cctcaaatcc cacgctgacg
    30841 ccgccgggat cggtttttac cttcagaaag cggtttgtcc gaagctgctc aaacatgggg
    30901 ggacgcttaa tggcgctctg cgttcgcctg cgggtgtcaa tttccaggta ttcttcaata
    30961 tcggctttgt ggaacgagcg tattccccgg cgctccgtgt cgtaaccggt gatcatgggg
    31021 ccgtaccggc cggttgttgt ccgccagttt ttcagtcgtc tcacctgagt gccgttccag
    31081 atgaacacca cacctgcctg gacacggaat acacgttttt tacgggcggg ccaccggtta
    31141 ccgtccggtg attgttgcga acgtatacgc ttttgttgcg cggcgcggac agcctgaccg
    31201 acagcacggg ctgttttgtt tataccggca gcggacagac cactgaggat ctcctgaaag
    31261 agaccgtcca gtctgtgcag gtcatccgga gccatcattc tgaccccatg tgacatcttc
    31321 gagggtgacg cgccatgctt ccagggcccg gctgacaggc ggctctccga tgtgttctgc
    31381 gtgaacagtg ccatcatcat tacggcggat aatcacccgc tcccataccg ggatttccag
    31441 caggatatcg gtgctgtcgt cgtcattgat ggcaacagaa aatttgatat ccctgttttt
    31501 ctccggatta agcaggagat caggctgatt gcggtacagc cagcgcatca gcggcaccat
    31561 cagttcatcg agatcctgcg tgaagtccat gacaaagatc ccaagcgtgt aacggtaaag
    31621 atatgagggg gattcgccgg tcgtctcaat gcctccgtta ttcacataca cagtgaattt
    31681 ctccgggttg gcgcgacacc atgggcaggc gtcagtcatg gcgtcgcgca gggtgttaat
    31741 tttcagcatg ccccctcctg tgtgttatgg catgttctgg ttgttctgtt gtacagactt
    31801 ctgactgatt ttttcagcgg cgcgctcgtc aatttttcgc tgaaaataat ttctgataac
    31861 actgtaaccg ccgctgatca gatacagcgc gcagacggca gtacagaaat acagcatgat
    31921 ctgatgaatg aataacataa tcacctcaca caaatgattg acataacgat gtctgtcgtt
    31981 ttaaaaaaaa tgaataacaa taataaacac tctccataat tcctcttgcg ctcccgtcag
    32041 gtatcttctc ctctcagtct tgcctgccgg gagcctcttt tttcttatgc ggcgttgctg
    32101 ttattgatat ccaccgtcac atactcaatc agcaccgggt aggtgtcctg gttactggtg
    32161 atatccgtga tcagcagttt gtcgttttca acacctgatg ccgggcattt cgccagatta
    32221 aaggccgcct gtgtctgacc gttgatgacc ggtgtcacgc tgatgctgtt attgccggca
    32281 aaccggaaca ccacgttgtg ccagttatga ttaaatgccc cgaaggttcc cagttttgca
    32341 ttctgttgtt tgtgctgcat cagattcagg tttgcgctgt cgctctggat gaagaaggct
    32401 gccagcatat tgcttcctgt gcttccctcc ggaacaacgt tttcaggtag ctgagaaacc
    32461 ggccagtaca gagccatggc gaagcggttt gctgccagct gaacaccttc cgggattttg
    32521 aaacgaaagg caatttcccc gcctttctga agtaattcag ctgctttact tgcaggcaca
    32581 tcgtgggcga ttttccaggc tgtttgttgc ccctggactt tttccactcg catggcttta
    32641 ccgctctcag cagcagagtc atttacgacg gaggcttttc catctgcccc tgtccagccc
    32701 tgttctttca gtttcccttc ctgtgcgtct gcgcgataag cgacggccgt tgttcttgcc
    32761 ggtgatgact gcgtacctgc gcccggctga ggtgcaggtg tggagggctg cgggctttcc
    32821 gtggtggtgc tgtctgaacc ggccgtggcg ggaggtactg cgaccacgct gaacggtgcg
    32881 ggtgcacctt tcaggaaggc gacggtcttc ccggcatgca tcaggatcgc cgttgccagg
    32941 cggtcagaaa taatgccgcg gcgggcccat gaactgaaat gcgtggcgcg atctgaagag
    33001 gtccagtttc cgttactacg ggatgcagca ccgtaataac cggcttctgc caggtcagga
    33061 tcttcggcag gcaggtttgt tggcgtgtta gcacctttct catcggtcat caggggcaca
    33121 aaataaattt tttgcgcttc tttgttctga tatgcaccgt aaaccaccgg gtactgagag
    33181 gtatattgtt gtttccagat ataggtggta tcgccgcaaa tccagggaac aagtgatgcg
    33241 ttgcctccgg tgcactgtgc agcatatgcg ctgagatcgg cgcggaactg attcaccatg
    33301 ttcaggaata attgcgggtg ctgtgcgtat gttgctgatt tcagatcaaa ctctcccagc
    33361 atccagacca cggataacag gacgtttttg gggtttttat ccagcgcggc ttttgtacgg
    33421 gtaagcagat cgttatacag cggtttgccg acgccccaca gggatgattc ggccgttgct
    33481 cctgaagatt cactgaatgc cccctctgcg ccggaagtaa acgctgaccc tccacgacag
    33541 caaggtacca ggaggatccc ggcatttttc gggataaacg ccagtaattt tttggcaata
    33601 tgtaaaccct gtccgacgca gccatactga ccttttgaca gatcggcgtt cggatgatta
    33661 aggctgctca tatcctgtac atcatggaga cagtgatctg ccggaataat atcgttatag
    33721 gtacatgcag cgccgcccgg tgtcaccgta ctgcgacgcg ctaactgttt aattcgtgga
    33781 tcaggactgt cgaatgtatc cggtaatggc agcccctcac cgtatgccat gccattggac
    33841 tgaccagcca gtggcaccac ataataaaac tctggctcag ttgtgtgtcc agtgttgtat
    33901 ccgtcggtag tgcccggtac gggtttttcc tcgccttctg gtaccgggat ggctgatggc
    33961 aggtctctgt cagtgaccag tgcctgcatc aggtaaaatg gtgctcccgg ctctgatgct
    34021 gagacaggtc ccccaagaag tccccatcct tccctgagca tgggcgcaag tttttcggca
    34081 agttccttgt tcgtcattgc cgtcacaagc tgataatgtt tcggtaccat agtgactccg
    34141 ttatttttca tgagtgttca ttcgtgctgt ccgctgccgc tccagcccgg caatggcggc
    34201 tttatcggca ttacaactgt ccagggcatc taccagtcgg tctccccagt cagccacatc
    34261 agcccatatc atgtgttgcg gcagcaccgg taccggcgtc tgtctggtca gactttccgg
    34321 cacgctcccg caaatgacgc cggttttcag cggcgctggc gtgttgttgc aggctgtcag
    34381 caacatcagc agggggagaa gtgcgtgcgc actcatccgc ctgtaagttt ttccgcatcg
    34441 tttcacgtcg ttcttcccct ctggctgcac gctcctgatc ttcgcgccgg atagcggcca
    34501 gcgctgactg tgtctccgcc gtggccttaa gcaactcccg gacggtcgta ttgctctctt
    34561 tcacggcttt ttccagcgct tcataacggg tgctctcctg acctgagcga taggcccgga
    34621 tgaccaccat tgcctgcagc gaaatggtca gcagcagcgc acaaaggata atgttcagtt
    34681 tcatggctgc gctcccgcgt ctttcaggca ccagttctta aaatcattcc ggcggttaac
    34741 cagtccctgc gtgcgcgttc cgccactgtt cacaaaatct gtcagccggt aacacatgag
    34801 gcgccattgc cgtgtctgcg cgtgtttcca gatggtggtg cgttgcggcg tgccgttttt
    34861 gtttttaaac cacatcagat tccggcagcc caggttaaag gcggcgtccg tcatggcttc
    34921 gaatgcagac tgcggcatct gctgccccct gaaattctgt gtgatgcagt tctcagcgcg
    34981 gcgcatatcg ttgatccacc gcccggccac ttcctcctgt gtgtacaggc ggttttccac
    35041 gttaccggtg gatccgcatc cgaccgtacc gacccccacc agatcacggt agggtcgggc
    35101 ccggcactcc tcccatgtgg caattttgat ttgtgcctcg cgggaggttc tcagttctcc
    35161 gggagccagg gagatcccca gcgccacaat ggcggccacg gcgtaacgct taggtccggc
    35221 catcgtgctc atcctgcttc agaagcatca ttgcccggcg ctctgcgtcg ttcagctccc
    35281 ttccttctga ctgcataagg attttttcaa tcagttcgtt gcgccttgcc tgggcgatct
    35341 ccatcctgcg gcggtgaagc caggcacgaa gcagtgacag cgttcccagc acaaaaccgg
    35401 ccacggcaat tttttcgctc agcgtcatca cgccgatacc ggctgtcgtc actgacgccc
    35461 agaacgtgat gtgatcacaa agccgctgaa acattctcac ccccacaact ggatcatttc
    35521 acgccgggtc tgcccctgca gctccggcag ctccacttcc tgtccggcat gcagaaacat
    35581 gacggctgaa atccccggat tggcggccag taccgcctca gtcaccccct gcgtggtgcc
    35641 gtaatggcgc tgacagagta aatcgacggt gtcaccgtcc agagccatta ctttcatcag
    35701 accagctccg cataaatgcg ggtgacaccg cgtatgtctg atattgccca gcgggcatcc
    35761 cgccacaaat cctcaacctg catgtccagc gcgtcagccc tgcggtcgcc tttatcggtg
    35821 gtatccacat cccgggtgcg ctccagtaca aacgcgcggg tgagtgcgaa aacggccctg
    35881 cggtagcggt ggacgtgtac actctctccg ttcacctgcg tggccggaac gtcagccagt
    35941 gaggccgcgc cttcagccat ccgctcacgt ttccagagct ccagttcggc gatcacatgg
    36001 gcgaccgctt ccgttgctgc atgtaacaga cgggttgttg ttgtcctccc cggaatgcgg
    36061 gaagccaggc gaatttcatg cagtgagatc gccggccaga agtcacccgc gctcaccatg
    36121 gcgtcgccgt catcggtgtc ggtggtatct gtgtcagcgc tgttcacgcg gggtctggct
    36181 atcagactca tgcctgtctc cgtaaaaatc aggcggtggg caaccggtaa aaagaacgcg
    36241 aagcctgttc agatcaccgg ttgcgccgcc tgccgacgag gtgcggttcg ttaaatcagt
    36301 ttgttttgcg cgcggctttt ttctggctgg cagcgctttt cttttttgcg ctgctctttt
    36361 tcccgccggt ggtggttttc ctgcccgttg tggatgcagg caccacctga tttccgtctg
    36421 ccagtttcct gaccgcgcgc tccagcgtgg caatttcacg cttcactccc gaagccggat
    36481 tgatccgcat ggcttcacgt aacaggctca gcgacagacg cagatcatcg ggagaggagg
    36541 aggcgcggcg ggaaaatgcg cgtgccttgt gcagttttga cctgacctgg tccggcatat
    36601 cctgctcact gaccaggtgg ttcagctcat caagcatggc ggtgtacaca gacaaatccg
    36661 cctcgctgtc cgttgtggcc tgcgtcagaa cggcgtcagt aatctcttca gccagtaccg
    36721 tggcggcgtc acggccaaag ttatccggca gcgccagacc atggcggatc acgtactccc
    36781 cgatacgcag tgccagcggg tagtcacggc agtcaatggc ccacaccatc atggtggtga
    36841 tgacctcatc ctggcgtccg ctgtctcctg ccagggtgcc ttcaatccac ccttcaaagt
    36901 caggcaacag atgtttttta agctctgccc tggcgacgtt tgactgaata cccttcagct
    36961 gcatctgtgc cagccttaac tggtgaagga cctgttcatg tgcggttctt tcccgttgtg
    37021 tttcccgggc gttcatggtc acccggttgc gggccaccgc ctgttgcaca tagcgttgtg
    37081 ccggtgtcag catttctcag ctcctgttaa cgggcgcggc gcgcccgtcc gggttatgct
    37141 tcgggttgcg gctgtccgct ttcggcgttg ttgtccggat cttcacctgc gaaggtgatc
    37201 ccctcaatca gcgcgcattt tccgtagtct tccaccacgt agcagtcatt catggactgg
    37261 taagtcgcga tacggttgta ttccggctcc tcgcgaatga aacggcgcag tgagcctttc
    37321 tggaagtaga tcgacaggtt tttcagcgac gtgatgagca tggcgtcttt cgggaagtac
    37381 ggcgcaagat acgtctgaag accaccgacg gtacgggacg ccacaatcag ctgcccggca
    37441 acaagctcag tgttcgggtt ggtggtgctg atggcgttaa tcatgggaag acgcagtgta
    37501 ttgaacagat cccgccccat cagtacgacc agatccggtg cgtccttatg ccattcatcc
    37561 agcacggatg agcggatgtc gtgcaccatg gcatccgggt taccatagtc accttttgcc
    37621 accagtttgt tttcatcatc gcgacgggtc agggtcatcc ctttcaccac gcgggtggct
    37681 gccttgtcgc ggatgtgttt cagccagccc gggttgacgt cctgcaggag agggttggtg
    37741 gcacgatcgg aaatcagcgc gtgtgactcc ccgttgaaac caatcatgat tcgatccagg
    37801 gctatctggc gggtgatttg cgcgctcagc agttgctgaa agtttttctg gctgctccat
    37861 gcgtcaatct gtgcgtagct gatgaaggta tcgtagttca cctgctcgca gcggtaacgg
    37921 cgtgcagcca gatcagcaac tgatgcaggg ttgcggcgtt tcactccgtc gctcgttgcg
    37981 ttcgtgctgg caatggtgcc gctggtgtca atcaggactt tttcaccttc ctggtcatcc
    38041 acaccgataa aattgatttc ctgtaacagt ccggcgcttt ccttgatggc gttctccatc
    38101 ttctgctgaa tggatggatc cacgctgaag gttttaccca ggcgctctgt tgtgatacca
    38161 gccagcgatg cctgctgaat catatagcgt tccagactgt cacgtccttc acgggaaagg
    38221 gataaacggg ccataaatta ctcttctcct gtcagaattc agccagtgtc tggtcagtgc
    38281 cgccagcgga agtaaaacgc tgctgtggat cggcatcctg gtttctcagt tgcgctttca
    38341 gtgctgcaag ctcgtgactg agcgcatcca gcgccgtttt gtcgagggca tgtttctgct
    38401 ccagagtgct gaaacggtcg ataagatctg catgatcgga tgcgatgttt tccacaacgt
    38461 cgcgcagctg gctgaactgc tcgccatccg ctgtgcgtgt tttgctgatg agtgccatga
    38521 cgcggctgaa ccactgacgg ctctcttccg ttcgctgttc tgtggtggta agcatctctg
    38581 cttccatggc gacggtgaac atggagggtt ctgttgtgca cttactgaac tgcatgatgc
    38641 tggcgcgttg ctgtgcggca aacttcaggc gttcagtgcc cagacttgct ggcgtgtcgg
    38701 tcattgccag acccacaatg taaggcccct tgacggcgtc cagctcaggc aacagctcaa
    38761 tgctcgaata cacttttttc ccgtcagcaa tcatgttttt catgcggtcg gtgggttcaa
    38821 tttcggcata taacgctgta cgccctgcca gtttcccttc ggtgatttca tcagttgaca
    38881 gagcgatcag atcacccact gcgcagagtt cactggatgg ccacatcgaa aggacgtgct
    38941 caatgttcac ccgggaggcg taaacggacg ggtcatagtt ttctgcggcc tgacgcagca
    39001 tttcaccaga aatttcacgt ccgtcgatgg ttgaaccgga aacggcaacg cgaaattttt
    39061 tgcgctctgg cgtgtttttg cttcccatgg aaattctcca taacctggtc gtttttccca
    39121 tgatggcggc agataacggt gtgtctcaag gtgcttaagt tgtatggcga cggggacaac
    39181 gcgggctggt atcaggcggt attgctgagg cttaatcttg ccctgttaat gtcagggggt
    39241 gcaatgatcc aggatgtctt tattcgtatg cgggcgaaac agctttactg gcaggggtat
    39301 accccggcgg atatcgctca gctgatggga ataaaccaga acacgattta ttcgtggaaa
    39361 aaacgcgatg agtgggatgc cactcccccg atccagcggg tcacccagtc cattgatgca
    39421 cgcctgatac agctcaccgc caaaccggat aaaagcggcg gtgactttaa ggaaattgac
    39481 ctgctgacgc gccagctgaa aaaactgaat gacggtcagg gagaacaggc tgtttccggt
    39541 aagaagcccc gcaggcgcaa actgaaaaat cacttcaccg aagagcagat tgtcgcactc
    39601 agggaaaaaa tcctgccgtc actggcctgg catcagcgcg aatggtacga caaccgccat
    39661 caccgcaacc gcatgatcct gaagtcgcgt cagattggtg cgacctggta ttttgcgcgc
    39721 gaagcactcc ttgatgcact gcgtgatgat gtctccgccg ggtaccagcg caaccagata
    39781 tttttgtccg catcccgtcg ccaggcgtat cagttcaaac actttattca gcaggcggcg
    39841 ctggaagttg atgtcgaact gaaagggggt gacaagattg ttttgtctaa cggtgcagag
    39901 cttcacttcc ttggcacatc ggcagcaacc gctcagtcgt ataccggaca ccttaaattt
    39961 gatgaatttt tttgggtcag taactttgtg gaactcagga aggttgctgg ggcaatggcg
    40021 acactggaag ggcttacgcg aacctacttt tcaacaccgt caggtgaaac acatgaggcg
    40081 tatcagttct ggacggggga ccgctggaac agcgaacagc cacgctcaaa acgcagtgag
    40141 tttgatgttt cacatgcgca tctgaagggg ggcgtgctct ttcctgacga aacatggcgg
    40201 cagattgtca ctattgagga tgctgtcgct aacggatggc ggtacaccaa tattggtgtt
    40261 attcgcagcg aaaatacaga aagtgaattt cgtaatctgt acatgtgcga atttgtccgt
    40321 gatggtgagg cagccttcag cctgagtgcg ctcaccggct gcggtgttga cggctatgac
    40381 gaatggcctg actggaaacc ttttgctgcc cgccctatgg gggtgaggga ggtctggctt
    40441 gggtatgacg ccaacggcag cagcgggaag ggggactgcg gggcactgtc ggtgtgcgtg
    40501 ccgccccttg ttgccggtgg gcgttttcgc acggtggaaa ctgtcagggt tcaggggatg
    40561 gagtttgaac agcaggccgc actgattgag gaatacaccc aaaaatataa cgtgaaacat
    40621 atcggcattg atgtgaccgg cgggaacggg gagggggttt atcagattgt gaaaaagttt
    40681 ttcccgatgg ccgtaccgta caacttcaat atcgcctcca agcgggcact ggtcatgaaa
    40741 atgcttcagg tggtgcgtgc cggacgctgg gagtatgacc gcagtgaacg tgacctgatc
    40801 caggcattcg catcggtgcg aaaaatacag acacccggcg gctatatcac ctacgacacc
    40861 gaccgctccc gtggcgtcag ccatggtgac ctcgcctggg caaccatgct tgctgttatc
    40921 aatgagccaa ttggtgcgga gcgtgaggat aatcgcggct ttgcaatgga gttctgaaca
    40981 caatgagtaa acgcaaaaaa tacaccggaa agcgggggca ggattcgcta cacgatgcac
    41041 tgaaatcaac gcctgcactc agcgcattca cctttgatgg gccctatgaa gtcagccgca
    41101 tggatctcct ggataacatg tcatgtgcag ataacgggcg ctactttgat acccccatgg
    41161 actggaacgc tatcgcccgt gcatcccgtc gtgcgtcctg gcatcagtcg gctttgtact
    41221 tcaagcgcaa cgccctgaac gggtgctttg ttcctcaccc tctgctgtcc cgccaggcgt
    41281 tttcagcgtt tgcgctcgac tggtttgtgt ttggtaatgc ctatcttgaa gtgcggcgta
    41341 acaggctggg ggaaccactc ctgcttcgcc cggcactggc aaaatataca cgcaggggaa
    41401 gtgacctgga tacctactgg tatctcaacg acgacggtac ggagttcagt ttcaggaaag
    41461 ggactgtgtg ccacgtgctt aatcctgata ttaatcagga gatctacggg atgccggaat
    41521 atatcggcgg gttgctgtct gtcagcctgt caaactcagc ggatacattc cgcaagctct
    41581 actacgataa cggctctcat gccgggtgta ttatctatgt aggcacatct caggcgaatg
    41641 cggaaagtgt cgaggccatt aaaaaaacgc ttacggattc ccgtggtcgc ggggcgttta
    41701 aaaacctgtt tctacatgcg ccgggtggtg gcaaggatgg tgtgcaaata ctgccgttcc
    41761 agcagataac cgcaaaggat gaattcctga acatcaaggg gagcgcccgc gacgatatcc
    41821 ttgctgccca tcgtgtacca cctcaactga tgggggcaat gcctgacggg aatgcggcgt
    41881 ttggtgacgt tgaaaaagcg gcgagagtgt ttttcatcaa cgagttgcaa cctgtcatgg
    41941 aggccatgaa gcatgtcaat gaatggcttg gcgttgaggt gatgcgcttt aacccctatg
    42001 ccctgcttct ggataacgcc agctgacgcg cttcgcttgt ctgctgcttc gccggggcat
    42061 aaaaaattta tgccccgact ctccagctct cttaacggaa tgcggatggc gttcttcttt
    42121 gcagcttgct gataatatgt atcttcatgc tgtttactat cggcagccat tccctgtcac
    42181 cgtctccgtt gatgaccacg cgaatggagg tatcatcttc cacgcgaacc cgaagagata
    42241 caccgtcttt tccgacggaa tgtaactcca ttgccaggac tatgtttacc gactctcctt
    42301 catcgtgtga gtcaacccat gttcttacag ccatccggaa tatgttatct gtgtcggttg
    42361 caatcatatt taccggaaat ggcctgaagt cccgatcgtt tctgcacgga aaccccgtat
    42421 caacgtaacg atgatgctcg ccgtctttgt cttcccatgt ctctgaaggc aattccagcc
    42481 actgctcata aaaagcaacc agcttttcag cctgattgcg cagaatccct gtaacctccc
    42541 gcctcgcgtc ccggctcttt ttgtttgcca ggtacaaatc gcgaaaatgg ctcatgtcgc
    42601 tctcctttgg tctgtttatg ccagcctgta ttctgcgctc acctcatatt gtgcgcaagt
    42661 tttcacccgt gaatgactga tactttaaat tctattaact ttatgatttc agtgattttt
    42721 gtctctcttt tttatcccgt tcgtcacatc cgtttccctc ccgatctccg acctcgccag
    42781 acgcaatcag acgcattctg acggcctgtt tccctgacca tgttttgatg tggtttttca
    42841 aaactgactt tactgcgtgc gtatcacgcg caatacgacc ccaaataaag caacccgcct
    42901 tccatccctc aattcgggcg cccttccctc gtcgcctgcg cgcagcgatc gcctcgtttt
    42961 tcgtgcaccg tgcaaagtgg cttcagccag cgccgttact gggctgaaag ggtaaaaaac
    43021 acataagaaa aattgtgcaa tttcgtgcac ttttgtgcgg ttttccaggt ggtgtttatg
    43081 cgggaatcgc aggctaaaaa aagcatgagc ggggattgcc cccgcgatat tcatgccgcc
    43141 cgatgagctt cacctactga cgggtctatt atgatttcaa aatccgtgaa caacgctttc
    43201 ggctgtacgc agttaccttg tttttccagg ctgacaaagc cgtatcgact cagcattttt
    43261 aacgttgctg acaaattact taactgcctt cctgaaagac tggcaagttc tgtcattgtc
    43321 tgcggttttt ccgcatccat gatacgcaga agcgcgatat tttcgttgct cagcgcctga
    43381 cccagagcgt tcagagatga aaaccagact cgcggttcgt caggttctgg ttttagtttc
    43441 cctttggcta tatccagcat tctctggcga aggactttct ccgggataat gccgatacgg
    43501 gctttcattt cttccccctt tgctgttcag caatcactct gtctacatgg ttaaagaaat
    43561 ccagtaaaag ctgttccgca gaagtgaatt catatggata gcctttatcg gacgatgttc
    43621 tgtgaatatg gtcataaacg gtaatgcgcc cggtaaattt ccctttcttt ggtggcttta
    43681 ttccgtgagc gttatccatt ccaaaaaccc gggagttata aggatcgtgc aatgtcaggt
    43741 tgtagcgtat tccatgtggt ctgaactggc ttgggctaac cctgtatgct tcaatcttcc
    43801 accagtaacc attatccatg tggtgctggt atccatgcat atccagaagg gtgttcagcc
    43861 catgatcatt gtttttcata actgattcgt atcaccttac ttatgaccaa atcataacat
    43921 cgtttttttc tttatcaagg tgggttattg tgctgttctt gtggttatcg acgttaactt
    43981 atgtacatat ttgaattcgt agtggttaat atgaaaaaag tgtctgacga atttgacggc
    44041 ttctgaatac gtaaaactgt gtcaggcgga gtatgtttct ccgccttttt gtttgtgcca
    44101 gcccggcgtc tcacatcatt actcacatat tgatcatatt gcggttcttg agctgataat
    44161 gctttgtgcc agcaactgcc agccttcttt catctgtccg tatttgtcct gatatctgtt
    44221 tacctgcaag ttcccgtgca cctgtaccag gtcgcctttt ttgtgttttg ccagttcttc
    44281 gtgctgcttc ccgaatgcga taaggtaaag ccacattgtg gttgactgtt ctgcgccatg
    44341 gccgggcagt gatatcgcca gtcgtgcagc tgttacatcg gtgcctctgg atgattttct
    44401 tgtcgtcggg tcttccgcca gtcgtccccg tgttgcaatc tgcgttgtca tatttgtctc
    44461 cgttggtggt ttagttattg gactgatatg actggcagat ctccgccagc gccgtagata
    44521 aacagaattc gcttcatgac ttcgctgcgc ttgcagactt cgatgactga tttttcctgt
    44581 tgccattcag cccgttgcgc cagctccagt cgttcctctt ccgttggctt ccggtaatgc
    44641 gttatcttgt ccgtgtaccc gtccacaacc agcaggcctt cccgtaccat ttttccggcg
    44701 gcctgattga tgacggttct tttcacgccc agtttttcta ccagttccgg tgcgctgaag
    44761 cgtgcgtgcg tttccaggta gcgtaaaatg atttcctttc cggtcattcc tggggtcttc
    44821 atttggtggc tccttcgttg ttccggcgct cccggagttc ggtcagtgct gccagtaaat
    44881 tttcccacca tgtttcttcc acgaccggat tttggcgtga aagcagctgt tgcgccgact
    44941 caatcatgct gttcaggtcc ctgtcgctga tcgccctggt gctgttaatg gtgtgttgct
    45001 gtctggtgct catgatgtct gtggttctcc tgttacttgc ggtgctgctt tctggcggct
    45061 gaatgtacat aacttgctat ggccgccttg cggttaatgc gcttcatcgc cctggctaaa
    45121 tcttcgttgt gtttcctgat atccttttcg tgatatgcct tcatgatcct cccggctggc
    45181 gtcagtggtt ctggtgctcc cgtgttcctc agtctcaggg ctgcgataaa gtcgccgctg
    45241 tctccccatg gtctggcgtt caggatgtcg gcagtcatac tttcggtact cctgtctgga
    45301 cgggctatgg tgatttccag ctggtcacct ggctgcggtc ttcttatcag tcctctcagc
    45361 gtcagcgccg gtaatctctt ggtcatgacg acgctgaact ctcgttgctg aagcgtgcag
    45421 ttgaatcgcg tcattcatcc cccttcagct gtgctacttc cttcaggctt ccggtgcttt
    45481 catcccagtg aacaatccgc ccgtcaggca ggcgaacgcg cgccccgcga tacacggaaa
    45541 gcgccgcact actgtgtaac tcctcgcccg ttgtactcag agtactggcg atcaacctgg
    45601 tggtcatatc cggttttgcg agtgccgcac cggcatatac cacgccttca ggtgcgggga
    45661 acccgccgct gtttgcggtt tttgtggatt tctggtcatt tttcagctgt tgggtgagct
    45721 gttttctctg tactggagtc agtgacctta accactcacc cggatcacct tccggtacga
    45781 ttagtttttg ttctttcctc ccttccggtg gtggtgtttt gtccgtttcg ttgtttgttt
    45841 tttcatcatg gcgacagtta ttgccacgag tccaaggtgc ggcaaagccg cactcaacgt
    45901 caaaaccctc gtccgtgcgg tcttcgtttt ccggcttacg tctgaccagt ttccaggtgt
    45961 ccgggtgggt gcatatggtg tagcgctccc cgagtgacgg ggcgcggacg ccgtaaatac
    46021 gttgcgggaa ttcgccgtaa tcgttggcct cttcggcagt ttcgtatgcg gtgcggacca
    46081 ggtacgtgtt gcggggaatg agcacaccac cctggcgggt gatataggtg gcaaaacatc
    46141 ccacgtcggc ggcggccagt atggcgtcaa tgtcgtcatg gtccagacgc tgcggggcag
    46201 ccgggttacg ggccatttgc ccggcaagac ggcggagttc gcgccatacc tggcggggag
    46261 ggataccgaa gaactggaac tggtggatgc ggtgaaggcc tgcccagccg atggcgttct
    46321 ccacggtttc ggccatggtt ttgcctgatt ccttatcgac gtaaggtttg ccggttttcg
    46381 ggtcattccc catgaaggcg ctggcgtcca gattcttacc gatataggtc gcgatatagc
    46441 ttgtcggtgt gccttttgtc gggtcgattt tttcgcattt aaaacgcggg gagatatcat
    46501 cacccagctc tgcgcggtcc tccctgatgg cggcgttgcg catgatttca gtgatacggg
    46561 cttcgttttc ctggctggtg aataccagca tgtgccagtg cggtgtaccg tcgtggtggg
    46621 gctccacggt gcgcacgccg taccagccca gcccggcctt gtcgatggtc ctgcggacgg
    46681 cggcgaaaaa gcggtaaacc agataatcac tggcatcgcg taccgttgct ccgcagtatt
    46741 tcgggttcag gctgccgtca tcgttcacgc tgtgaaaacg ggaaggggct gtgacggtga
    46801 cgaaaaacgc cacgtcgccg cgtgcttcgg cgacctgctc cattccccgg gcgcaggtca
    46861 tcatctcgtt acggcggtta cgcggattag agagacctgc ccagtagata tcctcaaggc
    46921 tgaaccgttc tccctcttcg ttctcgatgt cccaggcctt cagaaaatcg cgggtgcgct
    46981 ggcgtttttc gcgccagtcc agtaacgctt catggctgat atataccgat gtcttacgtg
    47041 acaccagacc tgcggcgcgt aacagttctt cacgccagat gtcccggagg tgtcttaact
    47101 gactgttcca ccatgtcgca caggaaaggc gcatgaccgc acccggcacc cgttccgggt
    47161 cgggctcggt gcggcggtcg ggcctgattt ccagcgacgg ccagcagggt ggcgtgatac
    47221 ccagttttaa cgccatgtgt gccagtggct ggtaggtcag taaccaggtg agcaggtcat
    47281 cgtcacccct gccggtcttt tcgatgacct catcacatgc gcgctccagt gcggtggaaa
    47341 acgcggtggc ggtcagggtg gcaaggcgct ggatttcgtc acggctcagc tgcggcagca
    47401 taagcagttc atccagccgt tcgcgggcgg cggtggcctt aaaggccggg gtggcaaagc
    47461 gttcattcac acgggcgata cgtttcaggc gggcacgcag cccgtgacga acgaagttat
    47521 ccgccgcata tgcgctgtta ttcttaagcc cttcccggcg tgcgctctcc tgtttgcggc
    47581 tcaggcgcgc caggtcacgg atgagcggac cggcaagaaa cggcggcagg gcgcgcagct
    47641 ctgccatggc ttcagatacc agcgtgggtg gattttcgcg tttgtgtcgc gtcagttttt
    47701 ccagtctgat ccgacgctct tcgtcgtgat gaagatgcga attaattcgc ttcccttcgt
    47761 ctgtggttgt caggaaggcg acggtttctt catccagtct ggcgtcagtg cgaatatcct
    47821 cgttgatgta gctctgaatg gcctggcgta atgcggttgg ctgcgctctg ccgaacgttt
    47881 caggatcgat aaccggtagc ggggcgttcc acgggtacgc cagctgtgca ttgtggtcac
    47941 tcatgcgtcg cactccccgg cagtcgccgc ctgaaacgcc gcgcggtaca gcacgagaag
    48001 gcgctgatat tcaccgataa agtcttctgc ggtgtatatg cctttcacgg ccacgctgtg
    48061 cgttgtcatt tccagaacaa gcgtcatctc tgacgacgac caactgatgg cggtgctgtc
    48121 ggttccgttg atgtgaaata aaccaaaacc atcacctttc acgtccacgc ggtaacggtc
    48181 gtcaacggtg aaggagaaat ccccgtatgt ggtgacgtgt ctggcggcct gtttacaggc
    48241 gcggcggtaa tagtcgtgcc agtcgttcag gcgggaagcc agccggggat ccactgccca
    48301 catccagtta aaaaaatcct gaatatctga aagtccctta acgctcttca tgagaacctc
    48361 cggtaacaga cgtgcggaag cctcccgcgc acgggtgcgg gatggcttca ggatgttgtt
    48421 tagtggtggt gttgctggtt gataaggtcc tgcagttctg ccaggtcttc ggccagatag
    48481 ctgaagactg ccgcgtaata cccggcagcg ataccgtcgt taagtcggtg taatacgttt
    48541 gactgcatga tgaaggagac agatgcagcc cgatggagtc tgcggttgat ttcggtgtgg
    48601 atgtgaagac gctcagcgcg ggcgcgctgt tgcttgcggt ttgccatggt gtggcctctt
    48661 gtgtagtaag ttttgaaaac tcaccatcca gaggttgcaa ttctgggtgg tgagacgtac
    48721 agggttgcaa ccaccggcta cacaagaccc cggcccgacc gaagtcggcc ctgtacgccc
    48781 caccataatt ctgacgcgaa aaagatgtga cgatgcggta cgcacaaaaa aaccgctggc
    48841 gcggttgtgc gcttgtgtag tcagcggggg ttgcaatccc ggcacccgtt ttatgaggtg
    48901 cgggaaaact gtaacccgac caggcgaacg ctggcaagcg gtttttgtgt tgcgtgctga
    48961 tgtggttaga atgtggcgaa ttaatatcac ctggcaactg gtgattgtct gcatacgcgg
    49021 ggtgtttctg atgagtgata agctcccttc tgattacgac attctttttg acctgtggta
    49081 cagctactgg cttgaacgta ttacatgtgt gtttcaccga cgcgtttctg ctgctctgcg
    49141 aatcagcgcc tttctggttg gtgttgcggc gtttgtcctg tgtgattttg ttgtgttggt
    49201 actcttcagt gtggttctgt ttctgctttg gcagtgcatg gctgccagtt ttgaacgcag
    49261 ggctgaaaag gctgaatggc aggcagtcag ttaccagcgc cttatcagtc agcgccatgt
    49321 gattagtaag gaatgcatga ttgcgcgcct ctcctttgtt gaggaaagtg acagtcccat
    49381 catggagtgc atgattaatc ttgcctggaa taaagcctgt gaggcggttg gcagctcttc
    49441 gcgcaatcat gtttcgatct ccggtcggtt actcagtcgc ctgtgcggct tttgatttgc
    49501 caagcagctg tgcgacagtt tcgccggccg cccgattttc cgggaatgac accggcatgt
    49561 tcagcttgag tgccattgcg tactcagccc gtgcgccagc ggactcttcc catccatcca
    49621 gtaagtaaat ggcatcggca cacatgagca tcggaataca aatgctcatg tactgctgct
    49681 ggcttaatcc gtcaggcagt acggcaggat taagaacaat gtttccggcg tacctaagct
    49741 tcaggtccgc caggcaaaac tccagtctgt tgtcgtcttt gtatccggtc atgggaccgg
    49801 cgatataaat cacggccatg ctttacctcc cgcgttgcgt gttgctgtca gcacgcgtac
    49861 ttcttccatg ttcgtggcgc agaagtgaga accgttgata aggcggatgt gatgcatctc
    49921 aggattgcgt gaatcctggc gtgtcatgcc ctggtcaatc aggctttcgt cgacttcgta
    49981 ctggcggccg ttggctgtga accggatgac ggttgactcc tggatcattg ctgacccctt
    50041 tgtccggcag atttctcgtt gagctgcttt ttaagggcgg cgatttcact ttcctgatcc
    50101 tgaatcaccc ggattgcttc catggtgatc cccagtgaga tcgaggaaga tataacgacc
    50161 tttggttttc caacggcagc cgcaactttg gctgtttcct cgtgtgcatg ggcgatgtga
    50221 cgtaaccccc gcaaaagtgc tttgtttctt tcgctattca tttcctgact cctgtgttac
    50281 tgattcatct gtttattcac gtactggcag gggtaaacat cacgcagtaa gttgcatctc
    50341 ccgttaccgg tccgcagtcc gggcagtagc caccgccata gtgaccgcag gcgtcgcact
    50401 gtctcagggt ggcgataact tccggtgctg gttcgcgggt tcttgcgcca acgctgcgtg
    50461 gtgcctgtat gcagttcatc cggtaaccct gataaatgcg tctggtttcc ggtgtgtcgg
    50521 aattggagat ggtgatgctg gtcatgccgc ccgtttcctg cgaaaggcgg tcaagtgctg
    50581 ccctcaggcg aacgtgctcg tgttcatcga atggttttcc gtaggcggtg aaattgtccc
    50641 ggccactgaa ttgcaggtaa gggggatcgc agtacacgca aagcgtgttg cccatgccga
    50701 acaggtgact ggcagagttc agcgtatcct taaaatctgc gtggataagc agcgttcggg
    50761 tgctgtttgc cttgtctgca aatgcgcgga tttcatgctc agggaaatag ggcttgcggt
    50821 agcagccgaa aggcacgtta aaatcgcctt ttccgttcac ccggtacagg ccattaaaac
    50881 agtgccggtt gaggtacaaa aacatggcgg ccagtcgcag ttcacggcgg tcgcttattc
    50941 ccgttctgct actcaggaag gcatgattct ctgcgttaaa ggtttcgcga atgatgagat
    51001 agtcacccct gttgttgtgg ttcaggaaca gggttttcgc ctcgtcgatc agactgtctg
    51061 tgttgtcccg tgctgcccgc cagaaattaa tcagcgcacc attggaatca cccaggatgt
    51121 agcgtttgta actggtgttc atgaataccg atgcgccacc cacgaacggt tcaaccaggc
    51181 agtcagcttc tggcagatgc accttcagtg tgttcatgat gcggcttttg ccacccaccc
    51241 atttcagcgg tgtgccggat aacagttctt tgctcataac gcggcctctt tgttataggt
    51301 ttcatgcgtt aataaatccc atgacttccc gttgttcttg ctgagtaatc gccagcgaaa
    51361 accgattctt atcaccaggt agttgtgggg ctttatgcgt gaaaaattcc gcacaccgtt
    51421 gcggaataaa cgcagcgatg cgcgggcttt tctttccacg ttcagggggg ggcagcaaca
    51481 cacattcagg atggcgacca tgcttcacct catatgttca ggaatggcgt tgtgaagcgc
    51541 tgccagattt ctgacacgac ctttgcctgg taaatggcat cgcccagtgc agtgtgagca
    51601 atagcctctt ttcctgatcg gcctggcatt ctccagccgg ttgattcagc catggcgata
    51661 agcggacgca tacagcgctc gtcccagtaa caccacggaa tacttttttc tccggttgtt
    51721 cgctttatgg cggatctcag gattgggaaa tcgaaggatg cgcccttgca ccatatggtc
    51781 agtgaattgc ccgggctttc gtcatgcata ttgcgtgaaa tgaacgccag aaattcggtc
    51841 actgctgcca gctctgttcc ctctgcgttg cgcaggcagg ccctggcctc cacggattgt
    51901 ccggcccacc atacagcggt tgttgcactg acggttccgc cgttgcgttc tgaatccacc
    51961 gggtcgatga atttctcaaa ctgcttgccc agttttccgg tgaacgggtc gaaaaacacc
    52021 gcgccgatcg cgaatatgac tgcgtccggt ccgttaccca gtgtttcggt gtcaatcatg
    52081 agatggttca ttgtacgggc tccttcggtt cgtcctggct ggtgagcatt gcaatcagtc
    52141 gttcaatgac tgaacaggtt tccagcgggg aggtgtgttt ttctttcgct gtctcggtca
    52201 gctcgcgcag taattcgcag atatgtgcac gctcggcttt tcttgcggcc atgctgatat
    52261 gcgcatgatg gttagctggt ctggcggtta tttctctgcc gggtgattcg gctgttctca
    52321 tttgttgact cctgaaaaaa agacaaaggg aacccgaagg catgaatgcc ttaattaatt
    52381 gctcgtgtgt tttctggtgt tattccgcgc ctgctttcgg cttcaggtta ttcccagatc
    52441 ttcagggcta accagataag cgcactcacc tgaaatatca cccatacagt ggcgatggtg
    52501 tgtattgctt ttacattctt cataaggttt ttgctttatt tacggcgttt atcgccggta
    52561 aagaataatt cgatatcctt cagtgtgtgg ataattcgca taatgccgat tgccataagc
    52621 acggagataa tcagaattac ccatgaaagg aatatgctca tgcggctacc cctgtgaagt
    52681 aaggctcgac aatttcacct tcaaacagtt ctgtcataat cgactttaat tcttccagcg
    52741 cttcacggtc atcgctgccc agtgcgcagt tgtatagccc cataattgcg ccgcgcagtg
    52801 agttacgaag gctttcatcc ttagttacgc cccggtttgc gagttcaaag tgataacgtt
    52861 gcaacgcgtt attgataagt attttgtagg cttcgatcat ggctgaatcc tttatttatc
    52921 agtgcttgct tcccggatat tgaaaacgca ggccgctgat taatctgtct gccgttctta
    52981 aggcttcaaa aaatgtgaag tcctgtccga agtaatcatc cccgcgagtc agggtgaaat
    53041 taaatcggcc agtaacaggg ctgcgaaaac ttttgtgaat aacgatgcca gtgtcgccaa
    53101 tcgcccaggt gtgcttgccg gtttgtttta ccgggtgacc gtctggtgtg gcatggattt
    53161 cagtggttgt ggtcgcacta tttttgcgga agtgctggcg tgcaacgtgg cgttcggcac
    53221 ttgttcgaaa attctgctgt gaaaaatttc ccggtttctt cataaaaatc cctactgctg
    53281 ctgtgaattt gtgccgggtt tttagccatg cccggcgcat ggttttgtag taatttcgtt
    53341 acgccttgca attcttgccc gaactgttgt aacgataacc aaccacacaa aggagtattt
    53401 atgttttctc ataaactatt tcggcaattt gcactgcatt gagactggta tcgagtaaac
    53461 cagtcgtcgt tttggcggta ttcgcagtgg aaatgttttg tcacgggaga tgttgatact
    53521 ctgcatcttg acgatgctgt cttccgttat tttttccttt atccactcaa gtaccgttac
    53581 ggcctcttcg taagaataat tattcagggc aatcaacatt gactctgttg catcctgtaa
    53641 tcgtttcaca aaataatccg gagatttctc tttattcata tgaaaattat ccttacatcc
    53701 gaatctgggc ggtggttttg ggttttgcgt gctgccgaac gtgagcttgc aagaagtgag
    53761 gggtttcaca gttttgttga tgcccgtgct catgcagaac tctttcgtat tggcgcgcgt
    53821 tcccctgtga ttcttcgctc tgtaaaaaaa tgtgctcagt cactggtgaa tgataaatat
    53881 caccttgtct tcttggttac ggaatctacg tctggttttt gcttgtcact ttcgtatcct
    53941 gaaaacattc acttgtatga tgaggtgttt gagtcttttg aaggtgccga atcgtttgct
    54001 caacatctct catacgatgt gttcgaactg gctgatattt atgattgttg ggggtcgctt
    54061 attcatccct tgcaacattc tcgtttttac agaaatgtgt ttgatattga tgatgatcat
    54121 ccatcagcct ggtaggctgc ttgcataaca accattttcc acagcatctg cggcgaaatc
    54181 tcattcctgg gatttcgctg ttgccggttt attttgatgt cactggttgc ctccactgta
    54241 ttggtgttgc tccagttttg actttcttcc tgtgtaaacc cgctttccat tgatggtctg
    54301 gcagtcttct ttgtttgccg gggaaaactt tcttttctgt cggtcgtatg tccagtagct
    54361 tttccagttg tgccattctg gcggctcgca ttctggtagt tcgtcagcca gcttgtccca
    54421 ctctctggcg ttaaaccacc acacgccatc cgaattttca tcttcagggt ttgaacgggt
    54481 cttgtgtcca ggcatctgat ttcgctgaag cgccacgcgc aatgcagctt ttgtcaaccc
    54541 gatataggct gccccttttt caagtgagca aagtccggct accgggccat ccagtcgcaa
    54601 ggttgcccct tttttgtagg tgcgtttttt cttttcctct tggtgttcat cgtcgtttgc
    54661 cgcgccaacg gattcggtga tgctgtcgtt ttcctgaagg gcttgtgtgt cagataactc
    54721 tttatcggca attgacttcc gcatttgcta tcctcttgcg ttggtgcgct tgtcgcgcat
    54781 catgaaggtt tgttttgatt tgtttacttc accctgtggt gaagttcgaa tagaggatac
    54841 tcatcgtatg gtgaacatgt caagagatta cgccaaaaaa ctaaaggcaa tcagaaacgc
    54901 ggaaaacctt acacaatctc agttttcgca agtaacggga gtttctgtag gaactatagg
    54961 aaattatgag tctggatata aacctgcgcg aattgaggtt gtagaaaaag taatccaagt
    55021 cgagcgattc gaaaaataca ctatgtggat acttcacgac aaaacatttc ccgctgccgg
    55081 gcaggttgcc ccggctctct ctcttgatgg ctccattcag tcggagggcg atcaggtttc
    55141 aatcggcatt acccaaaaat caccccgatc tcgtcgcaat gctggctaac gctgtacatc
    55201 gagcgggttt catcccgtag agagtgtacg gctgattcat ccgttggagg ggctaatcat
    55261 gtcgattaag caactcaaag acgggcgtta taaggttgat gtaagaccgc aagggacgga
    55321 aggaaagcgg atccgtaaga tttttaccct gaagtcaaag gcacaggagt ttgaaaggta
    55381 tgttttgcag aatttccacg ataaaccgtg gcaggccaag ccagctgatc agcgtcggtt
    55441 atccgaactg atagatgcct ggtgggtgct tgatggccgg aatcagcctt acggggatac
    55501 ttacagggtc aggctggaaa aaataaaccg tgagatgagg gacccccgcg ccagtgagat
    55561 gacgcgaaag tttatgctcg ggtatcgttc agacaaatta caggccggat taatgccctc
    55621 cagtgttaat cgtgatttgt gcgccttgtc tgcaatgttt acctcgctga ttgatgcgga
    55681 ggttttccat aacgaaaacc cggtgcgtgg aatacgtaag ctcaaggtca ggaatacaga
    55741 aatggccttt ctttcagatg atgagattga tcggctgctt gaacgactcg agggggatgc
    55801 gcgtcgtgtt gctgttctgt gtctttccac tggcgcaagg tggggggaag cagctggctt
    55861 acgtggtgag catatcgttg gtaaccgcgt aatgttcttt aacacaaaga atggaaaatc
    55921 ccgcgcggtt cctgtttctg atacggtgct ttcgttgata aaaacccgaa aaaccgggct
    55981 attgtaccag gctgattaca tccgttttcg ggatatcctt caggaggtga aaccggattt
    56041 acccaaaggt caggcgactc acgttatgcg tcacacgttc gccacgcatt tcatgatgaa
    56101 cggcggaaat atcgttacgt tacagcgaat ccttgggcac gccacgattc aacaaacgat
    56161 gacgtatgcg catttttcac cggacttttt acaggatgcg attagcttta atccgctggc
    56221 tgatagtgtc cataaactgt ccattgacta gttgtaattt gcgttaattt gctccccttt
    56281 ttttgattta gtcattgaaa aataagtaaa tcatcaaggg gggtaaggtg tgttaaatct
    56341 ccccggtggt ctgccctggc tgagtgaggc cgaccggcgg ctgcaggtgc agagtgattt
    56401 gccgtggtgg ctggtctgcc gggggacgat tcataagttc cgctgtgtgc cgcatctcac
    56461 cgggcggcgc tttgagcacg gggtgacgga ctgttacacg ctgttccggg atgcttatca
    56521 tctggcgggg attgagatgc cggattttca tcgcgaggat gactggtggc gtcacggtca
    56581 gaatctctat ctggataatc tggaggccac agggctgtat caggtgccgt tgtcatcagc
    56641 acaaccgggc gatgtgctgc tgtgctgttt tggttcatcg gtgccgaatc atgccgccat
    56701 ttactgtggt gatggcgagc tgctgcacca tattcctgaa caactgagca aacgagagag
    56761 gtataccgac aaatggcagc gacgcacaca ctccctctgg cgtcaccggg catggcgcgc
    56821 atctgccttt acggggattt gcaacgattt ggccgccgca tcgaccttcg tgtgaaaacg
    56881 ggggctgaag ccatccgcgc actggccaca cagctcccga tgtttcgtca gaaactgagc
    56941 gacggctggt atcaggtacg gattgccggg cgggacgtca gcacgtccgg gttaacggcg
    57001 cagttacatg agactctgcc tgatggcgct gtgattcata ttgttcccag agtcgccggg
    57061 gccaagtcag gtggcgtatt ccagattgtc ctgggggctg ccgccattgc cggatcattc
    57121 tttaccgccg gagccaccct tgcagcatgg ggggcagcca ttggggccgg tggtatgacc
    57181 ggcatcctgt tttctctcgg tgccagtatg gtgctcggtg gtgtggcgca gatgctggca
    57241 ccgaaagcca gaactccccg tacacagaca acggataacg gcaaacagaa cacctatttc
    57301 tcctccctgg ataacatggt tgcccagggt aatgttctgc cggttctgta cggtgaaatg
    57361 cgcgtggggt cacgcgtggt ttctcaggag atcagcacgg cagacgaagg ggatggtggt
    57421 caggttgtgg tgattggtcg ctgatgcaaa atgttttatg tgaaaccgcc tgcgggcgct
    57481 ttttttgtct ccctttgata ccgaacaata attactcctc acttacacgt aatactactt
    57541 tcgagtgaaa atctacctat ctctttgatt ttcaaattat tcgatgtata caagcctata
    57601 tagcgatctg ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgagc
    57661 aagcttgagt gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggtg
    57721 agtcgccgtg gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca
    57781 ttaacattac cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca
    57841 gacgaaaagg ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt
    57901 atgcacgtcg tggacggtga aatcaaatat gtcgaaacgg acaataccgg cgatgacaat
    57961 tacgacggcc tgcaccaggt tcgcgcctgt ttgcgtgggc gttccatgcg tcgccgtgtc
    58021 tataatccgg accgcctgaa atatccgatg aaacgagtcg gggcgcgcgg tgaaggcaaa
    58081 ttcgagcgca ttagctggga agaagcctac gacatcatcg cgaccaatat gcagcgcctg
    58141 atcaaagagt acggcaacga atccatctat cttaactatg gcaccggtac gctgggtggc
    58201 accatgaccc gctcctggcc gccgggaaat accctggtcg cgcggctgat gaactgctgc
    58261 ggcggctatc tgaaccatta cggcgactac tcctccgcgc aaattgcgga aggtctgaac
    58321 tatacctacg gcggctgggc agatggcaac agcccgtcgg atatcgaaaa cagtaagctg
    58381 gtagtgctgt ttggtaataa ccctggcgaa acgcgaatga gtggcggtgg ggtgacttac
    58441 tatcttgaac aggcacgcca gaaatctaat gcccgcatga tcatcatcga tccgcgctat
    58501 accgacaccg gtgccgggcg cgaagatgag tggatcccta ttcgtccagg aacagatgcc
    58561 gcactggtta acggtctggc gtacgtcatg atcaccgaaa acctggtgga tcaggcattc
    58621 ctcgataaat attgcgttgg ctatgacgag aaaaccctgc cagccagtgc gccgaaaaat
    58681 ggccactata aagcttatat tctgggtgaa gggccagatg gcgtggctaa aacaccggaa
    58741 tgggcctcgc aaatcaccgg tgttccggca gacaaaatca tcaaactggc tcgtgaaatc
    58801 ggcagtacca aaccggcgtt tatcagccag ggatggggcc cgcagcgtca cgctaacggt
    58861 gaaatcgcaa cccgtgctat ctcgatgctg gcgattctga ccggtaacgt tggtattaat
    58921 ggagggaaca gcggcgcgcg tgaaggttca tacagcttac cgtttgtccg tatgccgacc
    58981 ttggaaaacc cgatccagac cagcatttcg atgtttatgt ggaccgatgc cattgaacgt
    59041 ggcccggaaa tgacggcgct gcgtgatggt gtgcgcggga aagataagct ggatgtgccg
    59101 atcaaaatga tctggaacta tgccggtaac tgcctgatta accagcattc tgaaatcaac
    59161 cgtacccatg aaatccttca ggatgataag aagtgcgagc tgattgtggt tatcgactgc
    59221 cacatgactt catcggcgaa atatgctgac atcctgctgc ctgactgtac cgcttccgaa
    59281 cagatggact ttgcactgga tgcatcctgc gggaatatgt cttacgtgat tttcaacgat
    59341 caggtgatta aaccgcgctt tgaatgtaag accatctatg aaatgaccag cgaactggca
    59401 aaacgtcttg gcgttgagca acagtttact gaaggccgta cccaggaaga gtggatgcgg
    59461 catctgtatg cccagtcgcg ggaagcgatt cctgaactgc caacgtttga agagttccgc
    59521 aagcagggga tctttaaaaa gcgcgaccca caagggcatc acgttgctta taaagccttc
    59581 cgtgaagatc cgcaggcaaa tccactgacc acgccatcgg gtaaaattga gatttattcg
    59641 caggcgctgg ctgacattgc cgctacctgg gaattgccag aaggcgatgt gatcgatcca
    59701 ctgccgatct acacgccggg ctttgaaagt tatcaggatc cgctgaacaa acagtatccg
    59761 ctgcagctta caggtttcca ctataaatct cgcgttcact caacttacgg caacgttgat
    59821 gtgctgaaag cggcttgccg tcaggaaatg tggatcaacc cgcttgatgc ccaaaaacgc
    59881 ggtatccaca acggcgataa agtcaggatc tttaacgatc gtggtgaggt tcatattgag
    59941 gcgaaagtga cgccacgaat gatgccgggt gtggtcgcac tgggtgaagg tgcctggtat
    60001 gacccggatg caaaacgtgt cgataagggt ggttgtatta acgtactgac cactcaacgt
    60061 ccgtctcctc tcgctaaggg gaatccgtca catacaaacc ttgttcaggt tgaaaaggtg
    60121 taaggagtaa ccgatgacaa cccagtatgg attttttatt gattccagcc gttgcaccgg
    60181 ttgcaaaacc tgcgagctgg cttgtaaaga ctacaaagat ttgacaccag aagtcagctt
    60241 ccgccgcatt tatgaatatg ctggtggcga ctggcaggaa gataacggtg tctggcacca
    60301 gaacgtgttt gcctactatc tgtcgatttc gtgcaaccac tgcgaagatc cggcttgtac
    60361 taaagtctgc ccgagcggtg cgatgcataa acgtgaagat ggttttgttg tggtcgatga
    60421 agatgtgtgc attggctgcc gctactgcca tatggcttgc ccgtatggcg caccgcaata
    60481 taacgaaacg aaaggccata tgaccaaatg cgatggttgt tatgaccgtg ttgcggaggg
    60541 taaaaagccg atctgtgttg aatcttgtcc gctgcgggcg ctggatttcg gccctatcga
    60601 cgagctgcgt aaaaaacatg gcgatctggc ggccgttgcg ccgttgccgc gagctcactt
    60661 taccaaaccg aatattgtga tcaaacccaa tgccaatagc cgcccgaccg gggataccac
    60721 tggctatctg gcaaacccga aggaggtgtg agatgggaag tggatggcat gaatggccgc
    60781 tgatgatctt cacggtcttc gggcaatgtg tagcgggtgg ttttatcgtt ctggctttgg
    60841 cgctgctcaa aggcgacctg cgagcagaag cccagcagcg tgttatcgcc tgcatgtttg
    60901 gcttatgggt gctgatgggc attggcttta tcgcctctat gctccatctt ggttcaccga
    60961 tgcgcgcctt taactcgctc aaccgggtag gggcttcagc actcagtaac gaaatcgcca
    61021 gcggttccat cttttttgcc gtaggcggca tcggctggtt gctggcaatg ctgaaaaagc
    61081 tgtcaccggc attgcgtacg ctgtggctgg tagtgacgat ggttcttggc gtagtctttg
    61141 tctggatgat ggtgcgtgtc tataacagca ttgataccgt tccgacctgg tacagcatct
    61201 ggacgccgat gggcttcttc ctgacaatgt ttatgggcgg cccgttactg ggttacctgt
    61261 tgctgagtct ggctggcgtc gatggctggg cgatgcgtct gctgccagcc atttctgtac
    61321 ttgcactggt agtgagtggc gtgatgtcag tgatgcaggg cgcagagctg gcgactattc
    61381 atagttctgt gcagcaggcc gcagcgctgg tgccggacta tggtgcgctg atgtcctggc
    61441 ggatcgtgct tttggctgtt gccctgtgct tgtggattgc accacagcta aaaggttatc
    61501 agcctgcggt tccgctacta tctgtctcat tcattctgct tctggcaggg gaattaatcg
    61561 gtcgcggcgt attctatggt ttgcatatga ccgtggggat ggccgtcgca agctaatcag
    61621 taacaaccgg ggtttcggcc ccggttctct tttatttctg cttcgttaac gtgtcataac
    61681 tggtcatcaa attacgataa tcaggaatat ggttagagaa caacgtcgcc agtccttcaa
    61741 tatcattacg ccagtcacgg tgcagctcgc aagccacgcc gaaccatgtc atcaattgcg
    61801 cacctgcttg cgacatgcga tcccatgccg aatgccgggt aatttcatta aaggtccctg
    61861 acgcgtcagt cacaacgaag acatcaaacc cttcttcaat ggctgaaagc gccgggaatg
    61921 ccacgcaaac ttcggttacc acaccggcaa taattaactg ttttttacct gtcgctttga
    61981 ctgcttttac aaaatcttcg ttatcccagg cgttaatatt gcccgggcga gcaatgtaag
    62041 gtgcatcggg gaattgtgct ttcagttctg gaactaatgg gccgttagga ccggtttcaa
    62101 aactagtggt aagaatagtt ggtaagttga aatacttagc caggtcaccc agcgccagca
    62161 cgttattttt aaacttatcg ggttcgatat cccgtacaag ggaaagtaaa ccagcctggt
    62221 gatcaacaag caaaacggca gcatcatttt tatcaagacg aacatacggt ttggtcatcc
    62281 tcttctcctt tcgagagtca gtggtacgtc tgaaatcggt cagacgcgat gcattgctct
    62341 gaaagcatag acgggaaata tgagtttgct gtgaccatga aatttttcga ctgaacgaag
    62401 cgtatgaaat ttgtgttaga tcaataaaaa caatcagatg agattaatca tctcttcagt
    62461 attagcgtta tgaatgagta atataaatcg ataataccgc cgttgcgata tgcgcgtatt
    62521 ttcacccatt gacaatgttt ttggcggtgg catgatgcgc atgaaatttg aacttcctca
    62581 cggttttaat tcatgtccac gtatacccgg cctgtcatgc ttttgctgtc tggcctgctt
    62641 ttgttgactc tggcgatcgc ggtgttaaat acactcgtgc cgctttggct cgcccaggaa
    62701 cacatgtcca catggcaggt aggcgttgtc agctcatcct attttaccgg caaccttgtc
    62761 ggtacattgc tgacagggta tgtcattaaa cgcattggct ttaaccgcag ctattatctg
    62821 gcctccttca tttttgccgc tggctgtgcg ggccttggcc tgatgattgg tttctggagc
    62881 tggttggcgt ggcgttttgt cgcgggcgtc ggctgtgcca tgatttgggt agtcgttgag
    62941 agcgcgctga tgtgcagtgg gacgtcacgt aaccgtgggc gtttgcttgc tgcgtatatg
    63001 atggtttatt acgtgggaac gtttttaggc cagttactgg tcagcaaagt ctcaaccgag
    63061 ctgatgagcg tattgccgtg ggttacaggt ttgacgttgg cagggatctt accgctgttg
    63121 tttacgcgtg tcctgaatca gcaggctgaa aaccatgatt cgacgtcaat tacgtcaatg
    63181 ctaaaactcc gtcaggcgcg gcttggcgtg aatggctgca ttatctcagg aatcgttctg
    63241 ggatctctat atggcctgat gccgttgtac ctcaatcaca agggggtgag taatgccagc
    63301 attggtttct ggatggcggt actggtcagt gcgggtatcc tcggacaatg gccgattgga
    63361 cgtctggcgg ataagtttgg tcgactgctg gtgttgcgtg ttcaggtttt tgtcgtcatt
    63421 ctcggcagta tcgcgatgct tagccaggcg gcgatggccc cagcgttatt catcctcggt
    63481 gccgctggct ttacgctata tccggtggcg atggcctggg cttgcgagaa agttgaacat
    63541 catcaactgg tggcgatgaa ccaggcctta ctgttgagct ataccgtggg aagtctgctt
    63601 ggcccgtcat ttaccgctat gctaatgcag aatttctccg ataatttatt gtttatcatg
    63661 atcgccagcg tatcgtttat ctatttgctg atgctgcttc gcaacgccgg tcatacgccg
    63721 aaacccgttg ctcacgtgta aatgaattca agcagagtgt gaacttactg tttcacactc
    63781 tgcttttttg tttcttctat ctgacttgct tttttccaaa ttttattcgt ttaaaaataa
    63841 aatgtgcagc aggttataat tttgcatttc gctatttccg cacttcttat ttgccgcgca
    63901 taatccctcg ttttacagat gcccctttaa ttttggcgaa ggatttgtct atggctggga
    63961 atgttcagga aaaacagctg cgatggtaca acattgcgct gatgtctttt atcactgtct
    64021 ggggttttgg caacgttgtt aataactatg ccaaccaggg gctggtggtt gttttttcat
    64081 gggtgtttat ctttgcgctc tatttcacac cttatgcgct aattgttggt cagttaggct
    64141 cgaccttcaa agatgggaaa ggcggggtca gtacctggat taaacacacg atgggacccg
    64201 gactggctta cctcgccgcg tggacctact gggtggtgca tattccctat ctggcacaaa
    64261 aaccccaggc aatactgatt gcgctcggtt gggcgatgaa aggcgacggt tcgctaatca
    64321 aagaatattc agtcgtagcg ttacaggggt taacgctggt gctgtttatc ttctttatgt
    64381 gggttgcttc acgcggtatg aaatcgctga aaatcgtcgg ttctgtggca gggattgcga
    64441 tgtttgttat gtcgctcctg tatgtggcga tggcggtaac cgcgcctgcg attactgaag
    64501 tgcatattgc gaccacaaac attacctggg aaacgttcat tcctcatatc gactttacct
    64561 acattaccac tatttcaatg ctggttttcg cggttggcgg agcagagaag atttctcctt
    64621 acgttaatca aacgcgcaac ccaggaaaag aatttccaaa agggatgtta tgcctggcgg
    64681 tgatggttgc ggtttgtgcc attctgggct cactggcgat ggggatgatg tttgactcgc
    64741 gtaatatccc ggatgactta atgactaacg gtcaatatta cgcctttcag aagctgggcg
    64801 agtattacaa catgggtaat actttaatgg tgatttacgc cattgcgaat accctgggac
    64861 aagtagctgc gctggtattc tcgattgatg ccccgcttaa agtgctatta ggtgatgctg
    64921 acagcaaata tattccagcc agtttatgtc gtaccaacgc ttctggtacg cccgttaatg
    64981 gctattttct gaccctggtg ctggtggcga tcctgattat gctgccgact ctcggcatcg
    65041 gtgatatgaa caatctctat aaatggctgt tgaatcttaa ctcggtagtg atgccgctgc
    65101 gttatttatg ggtatttgtt gcatttattg cagtcgttcg cctggcgcag aaatataaac
    65161 cagaatatgt ctttattcat aacaagcctc tggcaatgac cgtcgggatt tggtgttttg
    65221 cctttaccgc ctttgcctgt ttgacgggga tcttcccgaa aatggaagcc ttcactgcag
    65281 agtggacctt ccagttggcg ctgaatgttg caacgccgtt tgtgctggta ggattaggac
    65341 tgatattccc gctgctggcg cgtaaagcga atagtaaata attattgtgg tcggtgacgc
    65401 tgactctggc agatctgacc cgtttttaca taatcgagaa atgcgcgcaa gccgcaggac
    65461 atatactggc ggtttgggta atacatttgg aagctgtgct gtaagggcgt tagaaataga
    65521 gctctgaatt atttatgcag cgcggaataa aaaagcggca actcaataaa gttgccgctt
    65581 tacggggaaa ttagaacatt accttatgac cgtactgctc aagaatgcct ttcacgcgtt
    65641 ccatggtttc tttcttcggt ggtttaacac cgtcgagttt gtattcttca cccatcgcca
    65701 cccatttgtg tttgcccagt tcgtggtagg ggagaagttc gattttctca acgttaccca
    65761 tatcacgggt aaattcaccg aggcgatgcg ctgaatcgtc atcgtcagac cagcctggga
    65821 caacaacgta gcggatccac accttcacat ttttattcgc cagatatttg gcgaactcca
    65881 gcgtgcggtg attggaaact ccaaccagat tttggtggat ctcatcgttc atctgtttga
    65941 gatcgagcat taccaggtcg gttacttcca gcagttcatc aatcaccgga tcgtaacgac
    66001 gaacaaaacc gttggtgtcc agacaggtat gaatgccttc ttttttgcag gcgcggaacc
    66061 agtcacgaac aaactcagct tgcaggattg cttcaccgcc ggatgcggta acgccgccgc
    66121 cggaagcgtt cataaagtgg cgataggtca ccacttcctt catcaaatct tcaacggtaa
    66181 cttctttacc gccatgcgtg tcccaggtgt cgcggttatg acaatacagg cagcgcatca
    66241 ggcagccctg gaaaaaggtg ataaagcgaa tacctgggcc gtctacggtt ccacaggatt
    66301 caaaggagtg aatgcgacca ataactgaca ttgcggtgtt tctccagatg tggcccatct
    66361 gaggccgtgt tggtgcgcag ctcgaaggct acgtcgagtc tgttttggca gtcaccttaa
    66421 agtatagata gctgacaaaa aaggctctcg tgctaaaaaa ggccccactt tcgtggagcc
    66481 tttattgtac gctttttact gtacgatttc agtcaaatct aattacatag attgagtgaa
    66541 ggtacgagta ataacgtcct gctgctgttc tttagtcagc gagttgaaac gtactgcgta
    66601 gccagataca cggatggtca gctgcggata tttttccgga ttttccatcg cgtcgagcag
    66661 catttcacgg ttcatcacgt taacgttcag gtgctgacca ccttcgatgg atgcttcgtg
    66721 gtggaagtaa ccatccatca gaccagccag gttggtctta cgaacttcgt cgtctttacc
    66781 cagtgcgttc ggaacgatag agaaggtgta ggagatacca tctttagcgt aagcaaacgg
    66841 cagtttagca acggaagtca gagacgctac agcacctttc tggtcacgac cgtgcatcgg
    66901 gttagcaccc ggtccgaacg gcgcgccagc acgacgaccg tctggggtgt tacccgtttt
    66961 cttaccatac acaacgttag acgtgatggt cagaacagac tgagtcggga tagcgtcacg
    67021 gtaggtgtgc agtttctgaa ttttcttcat gaaacgttct accaggtcaa cagccaggtc
    67081 atctacacgc ggatcgttgt taccaaactg cgggtattcg ccttcgattt cgaagtcgat
    67141 agccagaccg tcttcgtcac gaatcggttt aactttcgca tatttgattg cagacaggga
    67201 gtcagcagca acggacagac cagcgatacc acacgccatg gtgcggataa cgtcacggtc
    67261 gtgcagcgcc atcagagagg cttcgtagct gtacttgtcg tgcatgtagt gaatgatgtt
    67321 cagtgcagtg atgtactgtt tagccagcca gtccatgaag tgatccatgc gctccatcac
    67381 ttcatcgtag ttcaggagat cgcctttgat cggttcagac ttcggaccaa cctgcatttt
    67441 cagtttttcg tcaacgccgc cgttgattgc gtacagcatg gttttcgcca ggtttgcacg
    67501 cgcaccgaag aactgcattt gtttaccaac gatcatcggg cttacgcagc aagcgatagc
    67561 gtagtcatcg ttgttgaagt ccggacgcat caggtcatcg ttctcatact gcagagaaga
    67621 ggtgtcgatg gacactttag cggcgaattt cttgaagttc agcggcagtt tttcagacca
    67681 cagaatggtc atgttcggtt ccggagacgg acccatggtg tacagggtgt tcaggaaacg
    67741 gaagctgttt ttggtaacca gggtacgacc gtcgaggccc ataccaccga tagattcggt
    67801 tgcccagatc gggtcgccag agaacagttc atcgtactcc ggagtacgca ggaagcgaac
    67861 catacgcagt ttcatgacca ggtggtcaac catttcctgc gcttcttgtt cggtgatctt
    67921 gccagctttc aggtcacgtt cgatgtacac atccaggaag gtggaggtac gaccgaagga
    67981 cattgcagca ccgttctgag acttaacagc agccaggtag ccgaagtaag tccactggat
    68041 agcttcctga gcgttggtag ccggaccaga gatgtcgtag ccgtatttcg cagccatttc
    68101 tttcatctga cccagagcgc ggtgctgttc agcgatttct tcgcgcagac ggatagtctg
    68161 ttccaggttt acgccgtttt ccagatcagc ctgcagagag gtgaactgag cgtatttgtc
    68221 tttcatcagg tagtcgatac cgtacagcgc aacgcgacgg tagtcaccga tgatacggcc
    68281 acggccataa gcatctggca gaccggtcag aacaccggat ttacggcaac gcaggatgtc
    68341 cggagtgtaa acgtcgaaca cgccctggtt gtgagtttta cggtattcag tgaagatttt
    68401 tttgatcatc gggtccagtt cgcggttgta cgctttgcag gaaccttcga tcattttgat
    68461 accaccgaac gggataagag cacgtttcag cggagcttca gtctgcagac caacaacttt
    68521 ttccaacgct ttgttgatgt agccagcgtc gtgagaggtg atggtggaag caacagcggt
    68581 gtcaaagtca actggcgcgt gagtgcggtt ttccagttta acgccttcca ttactttgtc
    68641 ccacagggtg gtggtcgctt cagtagcgcc agccaggaag gactcgtcac cctcgtacgg
    68701 agtgtagttt ttctgaatga agtcacggac gtttacttca ttctgccagt cacctttggt
    68761 aaaaccttcc caggctgtgg ctaacttttc attaagctcg gacatgtaac acctaccttc
    68821 ttaagtggat tttttattta ctgcgtactt cgacaaccat taatggtgat cgttttcacg
    68881 caggtaaatg acccagtatg tcaacccaac caacaaacca ccgccgataa tattaccgat
    68941 cgtaaccgga atcaggttat cagtgatgaa gttcatcacg gtcaggtgag aaaaattttc
    69001 cggtgcagaa ccgacagcgg tccagaattc cggagatgcg aagtcgcgga ttacaatacc
    69061 catcgggatc ataaacatgt ttgcgatact gtgctcaaaa ccgctggcaa caaacatcgc
    69121 gaccggcagc accataataa acgctttgtc catcaggctg cggccagaat aactcatcca
    69181 tactgccaga cataccatca ggtttgccag gataccaagg cagacggcct caataaaagt
    69241 atggtgcact ttgtggtcgg cggtttgtag gacgtttagt ccccattgac catttgcggt
    69301 catatactcg ccggaaagcc acattaaaag tacaaacagc agtgcgccga ccaggttgcc
    69361 aaaatagaca tttagccagt ttttagccaa ctgaccccag gtgatgcgcc cactcgcctt
    69421 agcaacaaca atcaacactg tggaagtaaa gagatcggct ccgcagacaa cacaaagaat
    69481 cagccccaga gagaagcaaa tgccgccaac cagttttgcc atgccgaagg gcattgtgcc
    69541 tgtgccagtg gttgctgtga tatagaagac gaatgcgatt gagatgaaaa caccggcggt
    69601 aatcgccaga tagaaagtct taagcggatg tttcgttgct ttatagacac ccgcctcttc
    69661 ggccactttg gccattgcag caggaagtaa aagatcaaaa gggttgtcag ctttcacact
    69721 aactctctct ttattaagtc ggcgacgaga tactaacaaa gcattataga tgagaaattg
    69781 atatagatca tatctcgcct ggcttatagg cccgtaactc gc
//
LOCUS       DAZFKF010000016        67752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFKF010000016 DAZFKF010000000
VERSION     DAZFKF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.016"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            348..1199
                     /locus_tag="ACHKA1_001285"
     CDS             348..1199
                     /locus_tag="ACHKA1_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5678963.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPMFIMAHNRAQAVFLC
                     VMHSHIQIMVGRAGQPQGWPGSMMTGCSNPVRLTTHEIATSGGELMKFIMEAAIMATI
                     PALSHPEITIANGRAVTSSLAVAEYFRKPHKDVLAKISRLDCSAEFTERNFSPSEYTD
                     STGRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKELRQNNAPSPDKMI
                     HGDGRTLVIRLDEHGNIKFTETVPDGAMVCTLDTFQFYLEKQGWTLVNRSAIKNMTVE
                     QLLKIHC"
     gene            1210..1398
                     /gene="dicB"
                     /locus_tag="ACHKA1_001286"
     CDS             1210..1398
                     /gene="dicB"
                     /locus_tag="ACHKA1_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO5678964.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLELGIFGGQQ"
     gene            1395..1583
                     /locus_tag="ACHKA1_001287"
     CDS             1395..1583
                     /locus_tag="ACHKA1_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO5678965.1"
                     /translation="MSSAFALMMTVFLITGEPQNVITGIYASKGSCLQARDEQKISGE
                     CLPLNKVSLYLNNETPAG"
     gene            1679..4150
                     /locus_tag="ACHKA1_001288"
     CDS             1679..4150
                     /locus_tag="ACHKA1_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO5678966.1"
                     /translation="MSKIFICAAIPDEQAIKNEGAVAVATAIKAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGTHRPALDSWDAEYMLENRWDEESASFVPVETESDPM
                     NVTFDKLSPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPEIKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     KANQQYTENSTGKISPVIAAIHREYRQTWKTLDDELAYALWPGDVDAGNIDGNIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALLYSRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATDTLSSPVPETDAVETAIPDNEKTEYKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNSTYSGSQQNTGASDTTAQ
                     PEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVTQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHDSDNETGETADHS
                     IKVNGHQEITSTSRTCDHLMIDLEIMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWLYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARHQAKYVSAIWQKLIPNQADF"
     gene            4212..4481
                     /locus_tag="ACHKA1_001289"
     CDS             4212..4481
                     /locus_tag="ACHKA1_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5678967.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            4450..5568
                     /locus_tag="ACHKA1_001290"
     CDS             4450..5568
                     /locus_tag="ACHKA1_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO5678968.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            5845..6402
                     /locus_tag="ACHKA1_001291"
     CDS             5845..6402
                     /locus_tag="ACHKA1_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO5678969.1"
                     /translation="MTTQIPVETLSPIAHNQIPVITTELLAHLYGADVNSIQQNHKRN
                     SEHFIEGKHFFKVVGEELKNLRLTISQLQISPKTRSLILWTERGAARHAKMLETDQAW
                     EVFEKLEDSYFSQKQPPATQKSPIENDGCALLIHFNNYGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVTRKEQLVERLMRL"
     gene            6555..7349
                     /gene="potC"
                     /locus_tag="ACHKA1_001292"
     CDS             6555..7349
                     /gene="potC"
                     /locus_tag="ACHKA1_001292"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO5678970.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            7346..8392
                     /gene="potD"
                     /locus_tag="ACHKA1_001293"
     CDS             7346..8392
                     /gene="potD"
                     /locus_tag="ACHKA1_001293"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO5678971.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(8548..9369)
                     /gene="cobB"
                     /locus_tag="ACHKA1_001294"
     CDS             complement(8548..9369)
                     /gene="cobB"
                     /locus_tag="ACHKA1_001294"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO5678972.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(9385..10296)
                     /gene="nagK"
                     /locus_tag="ACHKA1_001295"
     CDS             complement(9385..10296)
                     /gene="nagK"
                     /locus_tag="ACHKA1_001295"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO5678973.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(10325..11569)
                     /gene="lolE"
                     /locus_tag="ACHKA1_001296"
     CDS             complement(10325..11569)
                     /gene="lolE"
                     /locus_tag="ACHKA1_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO5678974.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(11569..12270)
                     /gene="lolD"
                     /locus_tag="ACHKA1_001297"
     CDS             complement(11569..12270)
                     /gene="lolD"
                     /locus_tag="ACHKA1_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO5678975.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(12263..13462)
                     /gene="lolC"
                     /locus_tag="ACHKA1_001298"
     CDS             complement(12263..13462)
                     /gene="lolC"
                     /locus_tag="ACHKA1_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO5678976.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRFPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWHDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            13724..14797
                     /locus_tag="ACHKA1_001299"
     CDS             13724..14797
                     /locus_tag="ACHKA1_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO5678977.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVLQW
                     LALSALNQWLAPERDLSNASNAAYADSTGGFLHGMITASTSLWYLYALIVYFVLCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            14925..18371
                     /gene="mfd"
                     /locus_tag="ACHKA1_001300"
     CDS             14925..18371
                     /gene="mfd"
                     /locus_tag="ACHKA1_001300"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO5678978.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEVINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQNDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREVLRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            18515..19477
                     /gene="ldtC"
                     /locus_tag="ACHKA1_001301"
     CDS             18515..19477
                     /gene="ldtC"
                     /locus_tag="ACHKA1_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO5678979.1"
                     /translation="MMIKTRFSRWLTFFTFATAVALALPVKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(19559..19816)
                     /gene="bhsA"
                     /locus_tag="ACHKA1_001302"
                     /gene_synonym="ycfR"
     CDS             complement(19559..19816)
                     /gene="bhsA"
                     /locus_tag="ACHKA1_001302"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO5678980.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            20057..20689
                     /gene="comR"
                     /locus_tag="ACHKA1_001303"
     CDS             20057..20689
                     /gene="comR"
                     /locus_tag="ACHKA1_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO5678981.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELVK"
     gene            complement(20751..21290)
                     /locus_tag="ACHKA1_001304"
     CDS             complement(20751..21290)
                     /locus_tag="ACHKA1_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO5678982.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(21517..22821)
                     /gene="ndh"
                     /locus_tag="ACHKA1_001305"
     CDS             complement(21517..22821)
                     /gene="ndh"
                     /locus_tag="ACHKA1_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO5678983.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(23229..23771)
                     /gene="ycfP"
                     /locus_tag="ACHKA1_001306"
     CDS             complement(23229..23771)
                     /gene="ycfP"
                     /locus_tag="ACHKA1_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO5678984.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(23794..24819)
                     /gene="nagZ"
                     /locus_tag="ACHKA1_001307"
     CDS             complement(23794..24819)
                     /gene="nagZ"
                     /locus_tag="ACHKA1_001307"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO5678985.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(24830..25654)
                     /gene="thiK"
                     /locus_tag="ACHKA1_001308"
     CDS             complement(24830..25654)
                     /gene="thiK"
                     /locus_tag="ACHKA1_001308"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO5678986.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLHDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENAEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(25635..26276)
                     /gene="lpoB"
                     /locus_tag="ACHKA1_001309"
     CDS             complement(25635..26276)
                     /gene="lpoB"
                     /locus_tag="ACHKA1_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO5678987.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(26290..26667)
                     /locus_tag="ACHKA1_001310"
     CDS             complement(26290..26667)
                     /locus_tag="ACHKA1_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO5678988.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(26670..27029)
                     /gene="hinT"
                     /locus_tag="ACHKA1_001311"
     CDS             complement(26670..27029)
                     /gene="hinT"
                     /locus_tag="ACHKA1_001311"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO5678989.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            27363..29552
                     /gene="fhuE"
                     /locus_tag="ACHKA1_001312"
     CDS             27363..29552
                     /gene="fhuE"
                     /locus_tag="ACHKA1_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO5678990.1"
                     /translation="MLSTQFNRDNQHQAITKPSLIAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQTTLNATHSEVE
                     FDSKMMYVDAYINKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGRYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGTPRNFSITGTYQF"
     gene            complement(29612..31045)
                     /gene="ptsG"
                     /locus_tag="ACHKA1_001313"
     CDS             complement(29612..31045)
                     /gene="ptsG"
                     /locus_tag="ACHKA1_001313"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO5678991.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(31340..32137)
                     /locus_tag="ACHKA1_001314"
     CDS             complement(31340..32137)
                     /locus_tag="ACHKA1_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5678992.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(32148..33152)
                     /gene="holB"
                     /locus_tag="ACHKA1_001315"
     CDS             complement(32148..33152)
                     /gene="holB"
                     /locus_tag="ACHKA1_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO5678993.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYNTLAPEKGKNTLGIDAVREITEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(33149..33790)
                     /gene="tmk"
                     /locus_tag="ACHKA1_001316"
     CDS             complement(33149..33790)
                     /gene="tmk"
                     /locus_tag="ACHKA1_001316"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5678994.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRSRYLELAAQDNSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(33780..34802)
                     /gene="yceG"
                     /locus_tag="ACHKA1_001317"
     CDS             complement(33780..34802)
                     /gene="yceG"
                     /locus_tag="ACHKA1_001317"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO5678995.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(34805..35614)
                     /gene="pabC"
                     /locus_tag="ACHKA1_001318"
     CDS             complement(34805..35614)
                     /gene="pabC"
                     /locus_tag="ACHKA1_001318"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO5678996.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(35734..36975)
                     /gene="fabF"
                     /locus_tag="ACHKA1_001319"
     CDS             complement(35734..36975)
                     /gene="fabF"
                     /locus_tag="ACHKA1_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO5678997.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(37063..37299)
                     /gene="acpP"
                     /locus_tag="ACHKA1_001320"
     CDS             complement(37063..37299)
                     /gene="acpP"
                     /locus_tag="ACHKA1_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5678998.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(37510..38244)
                     /gene="fabG"
                     /locus_tag="ACHKA1_001321"
     CDS             complement(37510..38244)
                     /gene="fabG"
                     /locus_tag="ACHKA1_001321"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO5678999.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(38257..39186)
                     /gene="fabD"
                     /locus_tag="ACHKA1_001322"
     CDS             complement(38257..39186)
                     /gene="fabD"
                     /locus_tag="ACHKA1_001322"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO5679000.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WAMTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(39202..40155)
                     /gene="fabH"
                     /locus_tag="ACHKA1_001323"
     CDS             complement(39202..40155)
                     /gene="fabH"
                     /locus_tag="ACHKA1_001323"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO5679001.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(40223..41293)
                     /gene="plsX"
                     /locus_tag="ACHKA1_001324"
     CDS             complement(40223..41293)
                     /gene="plsX"
                     /locus_tag="ACHKA1_001324"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO5679002.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(41374..41547)
                     /gene="rpmF"
                     /locus_tag="ACHKA1_001325"
     CDS             complement(41374..41547)
                     /gene="rpmF"
                     /locus_tag="ACHKA1_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO5679003.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(41599..42120)
                     /gene="yceD"
                     /locus_tag="ACHKA1_001326"
     CDS             complement(41599..42120)
                     /gene="yceD"
                     /locus_tag="ACHKA1_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO5679004.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            42319..42903
                     /gene="yceF"
                     /locus_tag="ACHKA1_001327"
     CDS             42319..42903
                     /gene="yceF"
                     /locus_tag="ACHKA1_001327"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO5679005.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(43015..43974)
                     /gene="rluC"
                     /locus_tag="ACHKA1_001328"
     CDS             complement(43015..43974)
                     /gene="rluC"
                     /locus_tag="ACHKA1_001328"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO5679006.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            44547..47732
                     /gene="rne"
                     /locus_tag="ACHKA1_001329"
     CDS             44547..47732
                     /gene="rne"
                     /locus_tag="ACHKA1_001329"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO5679007.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQTQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVTAVKPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(47927..48880)
                     /gene="flgL"
                     /locus_tag="ACHKA1_001330"
     CDS             complement(47927..48880)
                     /gene="flgL"
                     /locus_tag="ACHKA1_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO5679008.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPGGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(48892..50535)
                     /gene="flgK"
                     /locus_tag="ACHKA1_001331"
     CDS             complement(48892..50535)
                     /gene="flgK"
                     /locus_tag="ACHKA1_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO5679009.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(50601..51542)
                     /gene="flgJ"
                     /locus_tag="ACHKA1_001332"
     CDS             complement(50601..51542)
                     /gene="flgJ"
                     /locus_tag="ACHKA1_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO5679010.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(51542..52639)
                     /gene="flgI"
                     /locus_tag="ACHKA1_001333"
     CDS             complement(51542..52639)
                     /gene="flgI"
                     /locus_tag="ACHKA1_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO5679011.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(52651..53349)
                     /gene="flgH"
                     /locus_tag="ACHKA1_001334"
     CDS             complement(52651..53349)
                     /gene="flgH"
                     /locus_tag="ACHKA1_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO5679012.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(53402..54184)
                     /gene="flgG"
                     /locus_tag="ACHKA1_001335"
     CDS             complement(53402..54184)
                     /gene="flgG"
                     /locus_tag="ACHKA1_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO5679013.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(54356..55111)
                     /gene="flgF"
                     /locus_tag="ACHKA1_001336"
     CDS             complement(54356..55111)
                     /gene="flgF"
                     /locus_tag="ACHKA1_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO5679014.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGLVLQADPTLRVMSGVLEGSNVNTVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(55131..56339)
                     /gene="flgE"
                     /locus_tag="ACHKA1_001337"
     CDS             complement(55131..56339)
                     /gene="flgE"
                     /locus_tag="ACHKA1_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO5679015.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLKFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            complement(56364..57059)
                     /gene="flgD"
                     /locus_tag="ACHKA1_001338"
     CDS             complement(56364..57059)
                     /gene="flgD"
                     /locus_tag="ACHKA1_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO5679016.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(57071..57475)
                     /gene="flgC"
                     /locus_tag="ACHKA1_001339"
     CDS             complement(57071..57475)
                     /gene="flgC"
                     /locus_tag="ACHKA1_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO5679017.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(57479..57895)
                     /gene="flgB"
                     /locus_tag="ACHKA1_001340"
     CDS             complement(57479..57895)
                     /gene="flgB"
                     /locus_tag="ACHKA1_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO5679018.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTTPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            58050..58709
                     /gene="flgA"
                     /locus_tag="ACHKA1_001341"
     CDS             58050..58709
                     /gene="flgA"
                     /locus_tag="ACHKA1_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO5679019.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNASVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            58785..59078
                     /gene="flgM"
                     /locus_tag="ACHKA1_001342"
     CDS             58785..59078
                     /gene="flgM"
                     /locus_tag="ACHKA1_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO5679020.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            59083..59499
                     /gene="flgN"
                     /locus_tag="ACHKA1_001343"
     CDS             59083..59499
                     /gene="flgN"
                     /locus_tag="ACHKA1_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO5679021.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(59539..61074)
                     /gene="murJ"
                     /locus_tag="ACHKA1_001344"
     CDS             complement(59539..61074)
                     /gene="murJ"
                     /locus_tag="ACHKA1_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO5679022.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(61184..62107)
                     /locus_tag="ACHKA1_001345"
     CDS             complement(61184..62107)
                     /locus_tag="ACHKA1_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5679023.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSAGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(62109..62756)
                     /locus_tag="ACHKA1_001346"
     CDS             complement(62109..62756)
                     /locus_tag="ACHKA1_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO5679024.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(62767..63351)
                     /gene="rimJ"
                     /locus_tag="ACHKA1_001347"
     CDS             complement(62767..63351)
                     /gene="rimJ"
                     /locus_tag="ACHKA1_001347"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO5679025.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            63587..64795
                     /gene="mdtH"
                     /locus_tag="ACHKA1_001348"
     CDS             63587..64795
                     /gene="mdtH"
                     /locus_tag="ACHKA1_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO5679026.1"
                     /translation="MSRVSQARSLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            64859..65506
                     /gene="grxB"
                     /locus_tag="ACHKA1_001349"
     CDS             64859..65506
                     /gene="grxB"
                     /locus_tag="ACHKA1_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO5679027.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            65640..66200
                     /locus_tag="ACHKA1_001350"
     CDS             65640..66200
                     /locus_tag="ACHKA1_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5679028.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            66306..67352
                     /gene="pyrC"
                     /locus_tag="ACHKA1_001351"
     CDS             66306..67352
                     /gene="pyrC"
                     /locus_tag="ACHKA1_001351"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO5679029.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESTCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            67426..67671
                     /gene="dinI"
                     /locus_tag="ACHKA1_001352"
     CDS             67426..67671
                     /gene="dinI"
                     /locus_tag="ACHKA1_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO5679030.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        16726 a        18021 c        16902 g        16103 t
ORIGIN      
        1 tgggggtttt tatttccttt cccctcattg aggacaccgg gttgtcaggt tgaccatatg
       61 cctgagtgac aaccccgcca caacatctcc atgtttcaat ggcagtggta cctttggcgg
      121 catcagttta ttagctttct gatgtccgcc ctttttaaag tgaattttgt gatgcggtga
      181 atgcggctaa gcgcacgcgg aacagttaaa accgtaaagt ggtcttttac ggggcgtaac
      241 gggcatcctt ctgtattccg gcgttaattg ttaactggtt aacgtcacct ggaggcacca
      301 ggcactgcat caacaaagtt cacttcggtg atgaagggta agagaaaatg ttgaatgtag
      361 ctattgaaaa ccagaacggg tggaattata gtgcacctgc acctcataaa acgggtgccg
      421 ggattgctac cccgatgttc atcatggcgc ataaccgcgc tcaggcggtt tttttatgcg
      481 taatgcacag ccacattcag attatggtgg ggcgtgcagg gcagccgcaa ggctggccgg
      541 gttccatgat gaccggttgt agcaaccctg tgcgtctcac cacccatgag attgctacct
      601 ccggtggtga gttaatgaaa ttcatcatgg aggctgccat catggctact atccctgccc
      661 tttctcaccc tgaaattact atcgccaatg ggcgtgctgt tacatcctct ttagctgttg
      721 ccgagtattt ccggaaacca cacaaagatg tactggcaaa aatttcacgt ctagactgtt
      781 cagcggaatt cactgagcga aatttttcgc ccagtgaata taccgactca accgggcgca
      841 aactccctat gtaccaaatc accaaaaacg gcttcgtttt cctggtgatg ggcttcaccg
      901 gcaaaaaagc cgctgcattt aaagaagcct acatcgctga gttcgatcgc atggagaaag
      961 aactgcgcca gaataacgcc ccgtctcccg acaaaatgat tcacggggac ggacgtaccc
     1021 tggttatccg tctcgacgaa cacggcaata tcaaattcac tgaaaccgtt ccggacggcg
     1081 caatggtctg caccctggat accttccagt tttatctgga gaaacaaggc tggactcttg
     1141 taaaccggag cgcaattaaa aatatgactg tggagcaatt actaaaaatt cattgttgag
     1201 gacgcgataa tggaaacgtt attaccaaac gttaatacgt ctgaagggtg ttttgatatt
     1261 ggtgttctgc tcagtaaccg ggagtttact gaagatgcca ttaagatgag aaaatatgag
     1321 ccttatcttc tcaatgataa ttccatactc tccagaattg cccttcttga acttggtatt
     1381 ttcggagggc agcagtgagt tcagcgtttg cactgatgat gacggttttt cttataacag
     1441 gtgagccaca gaatgtgatt accggaattt atgccagtaa aggatcctgc ctccaggcaa
     1501 gagacgagca aaaaatttct ggtgaatgtc tcccgttaaa caaagtatcg ctgtacctga
     1561 ataacgaaac accggctgga taacccacca gccatattaa cgccatacca acggattaaa
     1621 aatgccagca atggcaggga ttcgttcacc ctgaaatctg taatgaggtt taaacacaat
     1681 gagtaaaata tttatttgcg ccgccattcc tgacgaacag gcaataaaaa acgagggcgc
     1741 tgttgctgtg gccactgcca ttaaagccgg tgatgaacgt cgcgcccgcg caaaatttca
     1801 ctggcaattc cttgaacatt atccggctgc tcaggactgc gcttataaat ttcttgtctg
     1861 tgaggataaa cccggtacac accgcccagc cctcgactcc tgggatgctg aatatatgct
     1921 ggaaaaccgc tgggatgagg agtctgcttc ctttgtcccg gttgagactg aatcagatcc
     1981 tatgaacgtc acttttgaca agctgtcccc tgaagtacag aacgctgtca tggttaagtt
     2041 cgatacatgt gaaaacatca ccgttgatat ggtgattagc gcacaggaat tgttgcagga
     2101 agacatggca accttcgacg gacatatcgt tgaagcgttg atgaaaatgc cagaagttaa
     2161 cgccatgtat ccggagatta aactgcatgc catcgggtgg gttaagcata aatgtaagcc
     2221 tggtgccaaa tggccagaaa ttcaggcaga gatgcgcatc tggaaaaaac gtcgcgaagg
     2281 tgaacgcaag gaaaccggaa aatacacgtc tgttgttgat ctcgcccgcg ccaaagccaa
     2341 tcaacagtac actgaaaatt caacaggaaa aatcagcccg gtcattgctg ccattcatcg
     2401 cgaatacagg cagacatgga aaacactgga tgacgaactg gcctacgctc tctggcctgg
     2461 tgatgtggat gccggaaaca ttgacggcaa catccatcgc tgggcaaaaa aagaagttat
     2521 cgacaacgac cgcgaagact ggaagcgtat ctcggcatca atgcgcaaac aacctgatgc
     2581 acttctatac agccgtcaga ctattttcgg ccttgttcgt gaacgtccga tcgacattca
     2641 caaagaccct gtggcactga acaaatacat tactgaatac ctgactacaa agggcgtgtt
     2701 tgaagatgaa ggaacaaatc agagcgcaac tgatactctc tcgtcgccag taccagaaac
     2761 tgatgcagtg gaaacggcaa ttccggacaa cgaaaaaacc gaatacaaag tggaagtcga
     2821 accatctgta gagcgtgagg ggccgttcta cttcctcttc accgacaagg atggcgaaaa
     2881 atacggtcgc gcaaacaaac tttctggtct ggataaggcg ctggctgccg gggctactga
     2941 aatcacgaaa gaagaatatt tcgcccgcaa aaacagtaca tactcaggtt cacaacaaaa
     3001 tactggtgca tctgacacga ccgcacaacc agagccggta aaagttaccg ctgacgaagt
     3061 aaacaaaatt atgcaggcag ccaatatcag ccagcctgac gccgataagt tgcttgctgc
     3121 ctctcgcgga gaatttgttg cagggattag cgacccgaat gatccgaaat gggttaaggg
     3181 gatccagacc cgcgattctg taaaccagaa ccagcatgaa tcggaacgga actaccaaaa
     3241 agcggaacaa aacagcccaa atgcgttaca aaacgagcca gaaacgaaac agcctgaacc
     3301 agtgacgcaa caggaagtgg aaaaagtctg caccgcctgc ggtcagaccg gcggcggcaa
     3361 ctgccctgat tgtggcgcgg tgatgggcga cgcaacatac caggaaacat tcgatgaaga
     3421 gtatcaggtt gaagttcagg aagatgatcc ggaggaaatg gaaggcgctg aacatccaca
     3481 caaggagaac actggcggca atcagcatca cgatagcgat aatgaaactg gcgagacggc
     3541 agatcactca attaaggtga acggtcatca agaaatcaca tccaccagca ggacgtgtga
     3601 ccatctaatg atcgaccttg aaatcatggg aaaaaatcct gatgccccga tcatctcaat
     3661 aggtgcaata tttttcgatc cgcaaaccgg agatatggga ccggaattta gtaagactat
     3721 cgatctggaa actgctggcg gagtcattga tcgggacacc attaaatggt ggcttaagca
     3781 atcacgcgaa gcgcaatctg ccattatgac cgatgaaatc ccgttagatg atgcactgtt
     3841 acaattgcgg gaatttatcg acgaaaactc cggtgaattt tttgttcagg tttggggaaa
     3901 tggagccaac ttcgacaaca cgattttgcg ccgttcatac gaacggcagg ggatcccctg
     3961 cccgtggctt tactacaacg atcgcgatgt acgcacaatc gttgagctgg ggaaagccat
     4021 agacttcgat gccagaacgg ctattccatt cgaaggtgag cgccataatg cacttgatga
     4081 cgctcgtcac caggcaaaat acgtttcagc tatctggcaa aaactgatcc cgaatcaggc
     4141 tgatttttaa tgttcaaccg tcgccagttg tcgttgatat tctccaactg gcgcgttccg
     4201 gagtgatagc catgagcgaa cagtacctga taacgctcga cgagtggaaa ccaaaacggt
     4261 tcagtctccc aataacaaac actaccctgg tgaaatacgg aaaactagga tacatcgttc
     4321 caagaccaca aaaaattcgt gggcgttggc tgatagatcg ccgagcagta tttgttgggc
     4381 ctggtgaaac gggaattgcg ccggaaattc atactggcga tgatgatgca ctgaaggaga
     4441 ttttaactca tgtcaccgag gccacgaaaa aacagcactg acgtagccgg tctttacgaa
     4501 aagtttgatc gcagaactgg cagagtttac taccagtata aaaatcctgt gactggaaaa
     4561 tttcacggac tcggaacaga caaaggtaag gcagaaaaaa tcgcttccac agccaatcag
     4621 cgaatagctg cagcagaagc tgaatatttc atgcgcaaaa ttgatgaaag tccgtcagca
     4681 acaaaacgtc ggggtatcag attaaaggca tgggttgatc gatatctgaa aatacaggac
     4741 acgcgactga aaaatggaga tattgcagct acaactcaca aagaaaaaac tcgaatggct
     4801 gcatacctgg tttcccgtct gggaaaccac ccattgaaag aactggaagt aagagacttt
     4861 gcattaatac tggatgagtg gctggataaa gacatggtca gcacagcgag agtaaatcgt
     4921 ggattatggg ttgatattta taaagaagca cagcatgcag gggaagttcc tcctggatgg
     4981 aatcctccgg aggctacccg taaaccgatc cctaaagtaa ccagagccag gctcaccatg
     5041 gaagactggc aaaaaattta caatgcaacg cctgaaaaac actttatccg taacgcaatg
     5101 cttcttgcga ttgttactgg tcagcgccgt gatgacattt gccacatgcg tttttcagat
     5161 gtgtggaacg aacacttgca tatcacccag ggaaaaaccg gaatgcgtct ggcgttaccg
     5221 cttacactac gctgtgatgc cattgggata acgttaaaag aagttattga tgggtgccga
     5281 gacagaatat taagtccata tctaatccat agtcggcacc agaaacaacc gaagccgatg
     5341 agtaaagaca acctgagcga ctactttgcc aaagcacggg atctggctgg gataattcca
     5401 ccagcaggaa aaactccgcc aacatttcat gaacaacgct ctctatcaga acggctgtac
     5461 cgtgcacagg gtatcgatac aaaaacatta ctaggacata aagtccaggc aaccaccgat
     5521 cgctataacg atactcgggg tcaggaatgg gttaagttgg ttatttgaca aaaataaccg
     5581 tccgggatta tagtctgcgc acgccagcaa aatctggcgt cgggattagg aaccccggat
     5641 attcaaccgc gacagaacac gccgcgagcg tgttttttat tgtcgtaagc gcacgcacat
     5701 ctgaataatg gtggggcgta tgggggagcc gaaaggctcg ccggttggtt gatccggtag
     5761 ttcctaaccc tgtacgtctc gccacccgat gattaggaac ctgaaggtgg taatgttttg
     5821 aaacatcaac cggagggcgt caatatgacc actcaaattc ctgttgaaac tctttccccg
     5881 attgcccaca accagattcc tgtcataacc accgagttat tggcgcatct ttatggtgcg
     5941 gatgtgaata gtattcagca aaaccataag cgcaattcag agcattttat tgaaggtaaa
     6001 catttcttca aggttgtggg cgaagagctt aaaaatttgc gactgacaat aagtcagtta
     6061 caaatctcgc ccaaaacccg ctccctcatt ctctggacag aacgcggggc tgcccgtcat
     6121 gcaaaaatgc tcgaaacaga tcaggcatgg gaagttttcg aaaaactgga agacagttat
     6181 ttcagccaga aacaaccacc agccacacaa aaatcaccca tcgaaaacga cggatgcgca
     6241 ttactgatcc atttcaataa ctacggacag gtcgagttca cggaaaaagt gcctgccgat
     6301 gcgatggtgt gtactctgga gcgttttaag ttttatctgg agcagcacgg ctggatcgtt
     6361 acccgtaagg agcaactggt agaaaggctg atgcgacttt aaaaatgagg ggctcgcatc
     6421 aggaaaaatg ttgtagaatg cgagcccctc cgttttgggg aaaagttttg gagagatttt
     6481 ggagaaaggg aaaaattgaa taaattcaaa atcctgaaag tgccccgcct tatgaataag
     6541 aaggtggaac tcgaatgatc ggtcgactgc ttcgcggcgg ttttatgacc gctatctacg
     6601 cgtacctgta tatcccaatc attattttga ttgtgaactc ctttaacagc tcgcgctttg
     6661 gcatcaactg gcagggtttt accactaaat ggtatagctt gctgatgaac aacgacagcc
     6721 tgttacaggc agcgcagcat tcactgacaa tggcggtgtt ttcggcgacg tttgctacgc
     6781 ttatcggttc actgacggca gttgcgctgt accgttatcg ctttcgtggt aagccgttcg
     6841 ttagcggaat gctgtttgtg gtgatgatgt caccagatat cgtgatggcg atttcgctgc
     6901 tggtgctgtt tatgctgctg ggtattcagc ttggcttctg gtcgctgctg ttctcgcata
     6961 tcaccttctg cctgccattt gtggtggtga cggtgtattc gcgcctgaaa ggttttgacg
     7021 tgcgaatgct ggaagcggcg aaagatctcg gtgccagcga atttaccatt ctgcgaaaaa
     7081 tcattctgcc actggcaatg ccagcggtgg cggcgggctg ggtgttaagc tttaccctgt
     7141 cgatggacga tgtggtagtt tcttcgttcg tcaccggacc aagttatgaa attctgccgt
     7201 taaaaattta ttcgatggtc aaagttggcg tatcgccgga agttaacgcg ctggcaacca
     7261 tattactggt gctgtcgctg gtgatggtta ttgccagcca gcttattgct cgtgataaaa
     7321 cgaaaggtaa cacaggggac gttaaatgaa aaaatggtca cgccacctgc tcgcggcggg
     7381 tgctctggca ctgggcatga gcgccgctca cgccgatgac aacaacacgc tgtatttcta
     7441 caactggacc gagtacgtgc cgccaggact gcttgaacag ttcaccaaag aaaccggtat
     7501 taaggttatc tattcgactt acgagtcgaa cgaaaccatg tacgccaagc tgaaaacgta
     7561 caaagacggt gcctacgacc tggtggttcc ttcaacctat tacgtcgata aaatgcgtaa
     7621 agaagggatg atccagaaga tcgacaagtc gaagttaagc aatttcagca acctcgatcc
     7681 agacatgctc aacaagccgt ttgacccgaa taacgactac tccattccgt atatctgggg
     7741 ggcgacggcg attggcgtta acggtgatgc ggtagatccg aaatccgtta ccagctgggc
     7801 cgatctgtgg aagcctgagt acaaaggcag cctgctgctg accgacgatg cccgtgaagt
     7861 gttccagatg gcgctgcgta agctgggcta ctccggtaac accaccgatc cgaaagagat
     7921 tgaagctgca tataacgagc tgaaaaaact gatgccaaac gtcgcagcgt ttaactccga
     7981 taacccggct aacccgtaca tggaaggcga agttaacctc ggcatgatct ggaacggttc
     8041 tgctttcgtt gcacgccagg cgggtacgcc tattgacgtg gtgtggccga aagaaggcgg
     8101 catattctgg atggacagcc tggcgatccc ggcaaatgcc aaaaacaaag aaggcgcgct
     8161 gaaattgatc aacttcctgc tgcgcccgga tgtggcaaaa caggttgctg aaactatcgg
     8221 ttatccaacg ccaaaccttg cggcgcgtaa gctgttaagt ccagaagtgg cgaacgataa
     8281 gacgctctac ccggatgctg aaaccattaa aaatggcgaa tggcagaatg acgttggcgc
     8341 agccagcagc atttatgaag agtattatca gaagctgaaa gcaggacgtt aattcagcaa
     8401 gcgggcggtg gtattaccgc ccgtttttta gttagcaagg atgtggctaa tgagtcagga
     8461 cagtaaagtt ttatcccgct ctttcgtcgg tattgtggtc cgcttgtagg cctgataagc
     8521 gtagcgcatc aggcaatgct tcctgcttta taatcccttc agcaactttt caacaaattc
     8581 tggcaccacc tggcttgccg ggccgtaata tttctcggca aattcattac caacctgact
     8641 cggttcaaga ttcagttcca cggtgtgcgc gccatgcagt ttcgcttcgt gaacaaaccc
     8701 agccgccgga taaacatgcc ccgaagtacc aatggcaatg aaaatatcgg ccatcgacaa
     8761 cgccatataa atttcatcca tgccgagtgg catttcgcca aaccacacta cgtgcgggcg
     8821 caagggtgcc gggaactggc agcaatggca tttatcttct ggggtaacgt ctcctgtcca
     8881 gtcgagaacc tgaccacttt gtgaacaacg aactttcagc agttccccat gcatatgaat
     8941 cacattggta ttacctgcgc gttcatgcag gttgtctata ttctgcgtca ccagcaaaaa
     9001 gcgatcgccg agagcatctt gcagtttagc cagcgcaaga tgcgcggcgt taggctgaat
     9061 ttctggctgc tgtagctgtc gacgacgggc gttataaaac gcttgcacca gttcaggatc
     9121 gcgatcgaaa ccttccggcg ttgccacatc ttcaacccga tgttcttccc acaggccatc
     9181 tgcggcgcga aaggtacgaa tacctgattc cgcagaaatc cctgcccctg tcagtacgag
     9241 tactcttggt ttttccattg cttccggcac cactttatct ctgaaaaaaa tacgctgacg
     9301 caaacgctcg cgcaggcggc gtttattttt acgaaaacga cttaaccgat gaccccgacg
     9361 cgacagcata gcaacctctg ttgtttaatc ggttagatgt aggaaggccg caccacgcat
     9421 tcctcccgcg tcaccgtggc gcgcgcgttc aatgcgcgga acacgagcta caggtaagag
     9481 atgacgaggc agcctgtccg ccagttgcgt tgtgattgcc gggaaattcg ataagccacc
     9541 accaatgacg accaggtcag ggtcaacaat ggtcaggata tttcccagac aaaccgctaa
     9601 taaatccaga taacgctcaa cgtgcgccct tgcctgctca tcgccttgat cataaagcgc
     9661 aataatttcg ggagcctgca acggttgatg ataatagtgt tgatacagcc acgcaaaacc
     9721 gcgaccagac agataatttt caatgcagcc atgctgacca cagccgcagc ggcgtaacgg
     9781 gaaatccaac cccatcatgg ttaacgcatc aaccggcaga cgcatatggc caaactcgcc
     9841 ggtaatatag cttttaccgg taatcggttt accgttgaaa atcagcccgc cgccaacgcc
     9901 ggtgccgaga atcaacccca tcaccagtgg atattgagta aattcatcat cccaggcttc
     9961 tgaaagggca aaacagttag catcgttatc aaggcggaca tcgcgatcaa gacgtgcgct
    10021 caggtcggca cgcagcggtt taccgctggc agcggggaca ttggcggcat acagcgtacc
    10081 atcttctgtt tccggcattc ccggaatacc gatgccgaca gaacctttac agccaaaacg
    10141 ttggtcagct tcagctacca gctcacacac tgcatctaaa aatgcgtcat agctgtcacg
    10201 cggtgtcggc acccgctttt cccactgcaa ctgccgaccg ctatcaaaca cgccaagcgc
    10261 aatttttgtt ccaccaatat caaacccgta atacattgcc gctccttgtt ttaatgtact
    10321 gcctttactg gccgctaagg actcgcgcag ggtcaatatt actggcgcgc cgcgccggat
    10381 accaacttgc caaaagactc agcaacaatg ctgtgaccag tacgtagaag acgtccagcc
    10441 agtgcaattc cgatggcagg aagtcaataa aatagatatc gctggagagg aactgatgac
    10501 cgatcagctt ttcaatccgc tcaataatcg gggtaagttg cagtgaaacc accacgccga
    10561 taatcacccc acacaggctg ccgaacagcc ctgccagcaa tccataccag acaaagatgg
    10621 cgcgaattaa accatctttc gcccccagcg ttcttaatac tgcgatatcg ccactcttgt
    10681 ctttcaccgc catcactaag gtggagacga tgttgaaaca ggccacgcca atcaccagta
    10741 ccatcgccag atacataatg gcgcggatca tctggatatc gcgatacata tagccgtaag
    10801 taccaatcca acttttaata taaacatagc tgttggtcac ttcacccgca tcgcgtacca
    10861 gcttattggc gttgaaaaca tccgtcattt taagggcaat acctgacacg ctggaaccca
    10921 tatcaagata ttgttgggca tccgccagcg ggatcatggc aaaactgtga tcgagttgac
    10981 cactcaactg caaaataccg gcaacgtgca aacgcacacg ttttggctgc atcagtttat
    11041 gctcgggatt cgagttgggg atcataatcg acacccaatc gccctgcttc actttcagcg
    11101 catccgccac gcctttgccg atgataattt gctgttcgcc cgctttaaaa ttgcgccagg
    11161 catccccctg aacaaacgag ggtaatgcgc tcagacgctg ttcctgttgc gggttaacgc
    11221 ccttcacctg gattgcgcgc agattcgcgc cactttccac cagcccggtg aaattgatat
    11281 acggcgcagc ggcggcaata cctggcactt tttgcacgtt atccagtgct tcctgccagt
    11341 tagtccacgg ttgatccacc gcctctattt cgccatgcgg caccaccgcc agaatgcggt
    11401 tattcagttc gcgttcaaag ccgttcatcg cgcttaagcc gacgatcaat actgccacgc
    11461 cgagggcaat gccaatggta gaaataacgg agatcagcga caccatgccg ccgcgtcgcc
    11521 gtccgcgact aaaacgcagg ccaattaata acgataaagg catcgccatt actccgcccc
    11581 catcaggctt agttctgccg tcaaacgacc atcacgcatt tctaactggc ggctcatacg
    11641 tttcgccagt tgcaggtcgt gagtaaccac caggaaggca gtgccctgca accgattcaa
    11701 ttctccgagc aactggaaga tgctgtcggc gttacgcgca tcgaggttac cggtaggttc
    11761 atccgccagt accagacgcg ggttattgac cagcgcacgg gcaatcgcca cacgctggcg
    11821 ttcgccgcca gaaagttcag atgggcggtg attcgcacga tgctccagcc ccaccgcttt
    11881 taacatctcc agcgcacggc tgttaatttc agcgggcttt ttcttgccaa tcagcagcgg
    11941 catagccacg ttttccaggg cggtaaaatc cggcagcagg tggtgaaact gataaataaa
    12001 gcccagcttc tggttgcgca gctctgcttt cgccgccgaa gacagtttgc tcatcggttg
    12061 accgttaaag atcacatcgc cggaggttgg tgtatccagc ccgcccagca ggtgcagcaa
    12121 ggtactttta ccggaaccag agctaccgac aattgccatc atttcacctt cgcccacgct
    12181 gaaactgaca ttgtgcagca catcggtttg cacactgcct tcctgatagc gtttgcacag
    12241 gttgtcgcat tgcaacagga tcttattcat aacgtaaagc ctcagcgggt tgtgtggcgg
    12301 cagcgcgcca tgaagggtaa agcgtagaca gcagcgcgat agccatcgcc accagcgcaa
    12361 taacaatgac ctgtaaaggt tcgatagcca ccggcagcgc cgcgccatca agcaggacgc
    12421 cgattatcgg catcagatta tttaactggc tggcaagcag tgcgccaagc gccgctccga
    12481 ggatcgcacc gataatcccg gcgctggccc cttgcaccat aaagaccatc atgatttgtc
    12541 gcggagttaa gccttgcgtt tgcaggatcg ctacttcgcc ctgcttctcc atcaccatca
    12601 gccccagcga ggtaataatg ttaaacgccg caacggcgac aatcaggctc agcagtaagc
    12661 ccatcatatt tttttccatg cgtacggcct ggaacagctc gcctttacgg tcacgccagt
    12721 catgccattt gctgccttca ggcagttttt gctgacttaa agagtcaact ttcagcggct
    12781 catccagcca caaacgccag ccggtaatat tgcctgccgg aaaacgcatc agacgcgagg
    12841 catcctcaat attcaccagc atttcatagc catcgacttc actgttggcg gcgaaagtgc
    12901 caatcacatt gaacaggcgc tggcttggaa tacgccccat tggcgtgaac tggctggcag
    12961 atggcaccat cacgcggatt tgatcgccgc gattgacgcc caactgtgag gcaagctgtt
    13021 caccgaggat aacattatat ttccccggct cgaggtcagt ttgtttcaca ttgaccagat
    13081 acggcgttag tggatctttt tgcgccgggt cgatacccag catcaccccg accgccacgc
    13141 tgcgcgcgct ttgcagtacc acatcaccgg tagtaatagg tgcaacgcga ttaacgccgt
    13201 ccagtttgac tgccgtttcc gggagttgct gcgggttaag agagccatgc tcagaagaaa
    13261 gaattgcctg tggcatcagg ccaaggatgt tgttttgcag ctcgcgctca aagccgttca
    13321 tcactgacaa tactgtgacc agcgccatca ccccgagggt aatgccgatg gtagaaagcc
    13381 aggagacgaa acgaccgaag cgatccgctg cacgcccacg catgtaacgc aggccaataa
    13441 atagagcgac aggttggtac atgaaatccg tctggttgct gtagcaaaga cacgagtata
    13501 taagcgaaag caggatgggt aaacgtctga actgtaagtg atcgtcaggg ctaatctgaa
    13561 aagcccccgt aaatcaatcg gctaaattgc catgttttat catcaatctt gccgcctgat
    13621 cttaatctga aaaatcatcc aatacagaca gatacccatt acacctcggg tcataacccg
    13681 caggatcaac cgcatgtaaa ttaaaaaaga gcgtgttgtc ctgatgaaac aaaaagagct
    13741 atggattaac cagatcaaag ggttatgtat ttgtctggtc gtgatttatc actcggtcat
    13801 taccttttat ccgcatctga ccacttttca gcatccgtta tcggaagtcc tgagcaaatg
    13861 ctggatctat ttcaatcttt accttgcccc ctttcgtatg ccggtttttt tctttatttc
    13921 cggctatttg attcgccgct atatcgacag cgtaccgtgg ggaaattgtc tcgataaacg
    13981 gatctggaac atcttctggg tgctggcgct ttggggcgtg ctgcagtggc tggcgttaag
    14041 tgcgctgaac cagtggctgg cacctgagcg cgatttaagc aatgcctcca atgccgctta
    14101 tgccgattcc accggtgggt tcctgcacgg gatgatcacc gccagcacca gcttgtggta
    14161 tctgtatgcg ttaattgtct atttcgtgtt atgtaaaatt tttagccgtc tggcgctgcc
    14221 actattcgcc ttgtttgtac tgctgagtgt ggcggttaat ttcgttccca cgccgtggtg
    14281 gggaatgaac agtgtgatcc gcaatttgcc ttattacagc cttggcgcat ggtttggcgc
    14341 aacaataatg acctgtgtta aagaggtgcc gttgcgccgc catctgctga tggcttcttt
    14401 gctgaccgtt ctggcggtcg gtgcctggtt gtttactatc tcgctgctgt tgtcgctggt
    14461 atcgattgtg gtgatcatga agctgtttta tcaatacgag caacgattcg gtatgcgctc
    14521 caccagcctg ctgaatgtga ttggctccaa caccattgct atctacacca cccatcgcat
    14581 tctggttgaa atattcagct taactctgct tgcgcaaatg aacgcagcac gctggtcgcc
    14641 gcaagtcgaa ctgacactcc tgctggttta tccctttgtc agtttgttca tctgtactgt
    14701 tgcgggcttg ctggtaagaa aactttcaca gcgtgcattc agcgatctgt tgttctcccc
    14761 gccttcactg cccgctgccg tcagttactc ccgctaagcc atcgcccccg ccggggcgat
    14821 tcgatttgct aaagcatgtc gattacggat aatgaacagc attgcttatc agagatatgc
    14881 ccccatatgt tgaggcatat cctaacgaga atctgacaac cgttatgcct gaacaatatc
    14941 gttatacgct gcccgtcaaa gcgggtgagc agcgtctgct gggcgagtta accggcgctg
    15001 cctgcgcgac gctggtggcg gaaattgccg aacgtcacgc cggtccggtg gtactcattg
    15061 caccagatat gcaaaatgct ctgcgtttgc atgatgaaat cagccagttc accgatcaga
    15121 tggtgatgaa tctggcggac tgggaaaccc tcccttacga cagtttttcg cctcatcagg
    15181 acattatctc ctcgcgcctt tccacccttt accagctacc gacgatgcag cgtggcgtac
    15241 tgattgttcc ggtgaatacg cttatgcagc gcgtttgccc acacagtttt ctccacggtc
    15301 atgcgctggt gatgaaaaaa ggtcagcgcc tgtcacgaga tgcattacga acccaactgg
    15361 acagcgccgg ttatcgccat gttgaccagg tgatggagca cggcgaatac gccacgcgcg
    15421 gcgcgttgct ggatctcttc ccgatgggga gtgagctgcc ttatcgtctt gatttctttg
    15481 atgatgaaat cgacagcctg cgggtgtttg acgtcgacag ccagcgcacg ctggaggaag
    15541 tagaagtgat caatctgctg cccgcgcacg aatttccgac cgataaagcg gcaattgaac
    15601 tgttccgcag ccagtggcgc gataccttcg aagtgaagcg cgatccggaa catatttacc
    15661 agcaagtgag taaaggcaca ttacctgccg ggatcgagta ctggcagcca ttgttcttca
    15721 gcgaaccact gccgccgctg ttcagttatt tccctgccaa taccttgctg gtgaatactg
    15781 gcgatctgga aaacagtgcc gaacgtttcc aggctgacac gctggcgcgt tttgagaatc
    15841 gcggcgtcga tccgatgcgc ccactgttgc caccacaatc gctctggctg cgggtggacg
    15901 agctcttctc agagctgaaa aactggcccc gagtgcagct aaaaactgaa catttaccga
    15961 caaaagccgc gaatgccaat ttaggtttcc agaaactgcc agacctggcc gttcaggcac
    16021 aacaaaaagc gccgctggat gcgctgcgta agttcctcga gtctttcgac ggtccggtgg
    16081 tgttctcggt agaaagtgaa ggtcgccgtg aagcgctggg tgaactgctc gcgcgaatta
    16141 aaattgctcc gcaacgcatt atgcgtcttg atgaagccag cgaccgtggg cgttatttga
    16201 tgattggcgc tgccgaacat ggttttgtcg ataaggtgcg taacctggcg ctgatttgcg
    16261 aaagcgatct gctcggtgaa cgcgttgccc gccgtcgtca ggactctcgc cgcaccatca
    16321 accccgatac actgatccgt aacctcgcgg agttgcacat tggtcagccg gtggtccatc
    16381 tggagcacgg cgtcgggcgc tatgcaggaa tgaccacgct ggaagctggc ggcattaccg
    16441 gcgagtacct gatgctcacc tatgccaacg acgccaaatt gtatgttccg gtgtcgtcac
    16501 tgcatctgat tagccgttac gcaggtggcg cggaagaaaa cgccccactg cataaacttg
    16561 gcggcgatgc gtggtcacgc gcgcggcaga aagcggcgga aaaagtgcgt gatgtggcgg
    16621 cagaattgct ggatatctac gcgcaacgcg ccgccaaaga gggcttcgcg tttaaacacg
    16681 atcgtgagca gtatcagttg ttctgcgaca gcttcccgtt tgaaaccacg ccggatcagg
    16741 cgcaggccat taatgcggtg cttagcgaca tgtgtcagcc gctggcaatg gatcgtctgg
    16801 tgtgcggcga tgttggcttt gggaaaacag aagtggcgat gcgcgccgct ttcctggcag
    16861 tagataacca caagcaggta gcggtgctgg tgcctaccac ccttctcgcg cagcagcatt
    16921 acgacaactt ccgcgaccgt ttcgccaact ggccggtacg tatcgaaatg atctcccgtt
    16981 tccgcagcgc caaagagcaa acgcaaatcc ttgcggaagt ggcggaaggg aaaatcgata
    17041 ttctgatcgg tacgcacaaa ctgctgcaaa atgacgtcaa gtttaaagat ttaggtctgc
    17101 tgattgtcga tgaagaacac cgcttcgggg tgcgtcataa agagcgcatt aaagcgatgc
    17161 gtgcgaacgt ggatattctg acgcttaccg ctacgccgat cccacgtacg ctgaatatgg
    17221 cgatgagcgg aatgcgtgac ctgtcgatta tcgccacgcc gcccgcccgt cgtctggcgg
    17281 tcaaaacgtt tgtccgcgag tatgacagtc tggtggtccg ggaggcgatc ctgcgtgaag
    17341 ttctgcgcgg cggtcaggtt tattatctct acaatgatgt ggaaaacatc cagaaagctg
    17401 ccgaacggct ggcagaactg gtgccagaag cacggattgc catcggtcac gggcagatgc
    17461 gcgagcgcga actggaacgg gtgatgaatg atttccatca tcaacgtttc aacgtgctgg
    17521 tttgtaccac cattatcgaa accggaatcg atatccctac cgccaacacc attatcattg
    17581 aacgcgcgga tcacttcggt ctggcgcagc tgcaccagtt gcgcggtcgc gtcggacgtt
    17641 cacatcatca ggcatatgca tggctgctga cgccgcatcc aaaagcgatg actaccgatg
    17701 cgcaaaaacg tcttgaagcg attgcctcgc tggaagatct cggggcaggt tttgcgctgg
    17761 caacgcacga tctggagatc cgcggcgcgg gtgaactgct tggcgaagaa caaagcggct
    17821 caatggaaac catcggtttc tcgctgtata tggagttgct ggaaaacgcc gtcgatgcac
    17881 tgaaagccgg acgcgagccg tcgctggaag atctcaccag ccagcaaaca gaagtcgagc
    17941 tgcggatgcc gtcgctattg ccagatgatt tcatccctga cgtgaacacg cgtctgtcgt
    18001 tctacaaacg tattgccagc gccaaaacgg aaaacgaact ggaagagatc aaagtcgagc
    18061 ttatcgatcg cttcggcctg ctgccggatc cggcgcgtac cctgctggat attgcccgtc
    18121 tgcgccagca agcgcagaaa ctggggatca ggaagctgga aggtaatgag aaaggcggcg
    18181 tgatcgaatt tgccgagaag aatcacgtta atccggcctg gttgattggt ttgctgcaaa
    18241 aacagccgca gcattaccgc cttgatggtc cgacgcgcct gaaatttatt caggatttga
    18301 gtgagcggaa aacgcgtatc gaatgggtac gccagtttat gcgtgaactg gaagagaacg
    18361 cgatcgctta atggtaaatc gggggcgttt ctgcgccccc atctgcaaca tttacaaatt
    18421 ctttgcactt ccctgcacta tccgacactg tcaccatcca taattcaggc ttatctgttt
    18481 attacaataa ccttatattt attatggatt tttggtgatg atcaaaacgc gtttttctcg
    18541 ctggctaacg ttttttacgt tcgccactgc cgtggcgctg gcgctaccgg taaaagccaa
    18601 cacctggccg ctgccgccag cgggcagtcg tctggttggc gaaaacaaat ttcatgtggt
    18661 ggaaaatgac ggtggttctc tggaagccat cgccaaaaaa tataacgtcg gctttctcgc
    18721 tctgttacag gctaaccccg gcgttgatcc ttacgtaccg cgcgcgggca gcgtgttaac
    18781 gatcccgttg caaaccctac ttccagatgc gccgcgcgaa ggcattgtga tcaatattgc
    18841 ggagctgcgt ctctattact acccgccggg taaaaattcg gtaaccgtgt atccaatagg
    18901 tattggtcag ttgggtggtg acacgctgac accgacaatg gtgaccaccg tttcagacaa
    18961 acgagcaaac ccaacctgga cgccaacggc aaacatccgc gcgcggtata aggcacaggg
    19021 aattgagttg cctgcggtag tgccggctgg accggataac ccaatgggcc atcatgcgat
    19081 tcgtctggcg gcctatggcg gcgtttattt gcttcacggt acgaacgccg atttcggcat
    19141 tggcatgcgg gtaagttctg gctgtattcg tctgcgggat gacgatatca aaacactctt
    19201 tagccaggtc accccaggca ccaaagtgaa tatcatcaac actccgataa aagtctctgc
    19261 cgaaccaaac ggtgcgcgtc tggttgaagt acatcagccg ctgtcagaga agattgatga
    19321 cgatccgcag ctgctgccaa ttacgctgaa tagcgcaatg caatcattta aagatgcagc
    19381 acaaactgac gctgaagtga tgcaacatgt gatggatgtc cgttccggga tgccggtgga
    19441 tgtccgccgt catcaagtga gcccacaaac tctgtaaatc ctggataaaa aaatccccgc
    19501 gacacggcat gtcgcgggga aaaaatcagc agtagcaggc attaatgagg gttaatgctt
    19561 atttataaat tactgctgtt ccatggaggg tattcggacc ggttacagaa gtaatacgga
    19621 aagattttgc gcccatctca tccgcttttt gcgccagctg ctcttccagc gatcccagat
    19681 ttgtccccgc gttagcactg attgtaccga ctttttgttg gccttctggc gttgactgaa
    19741 cttcgacagc cgcaaagctg gcaaatgaca tggagcttaa aatcgccgca gcgatgagag
    19801 tttttacgtt tttcataata gtggccttat gcagatgaat gacgtcgcaa gcaattactt
    19861 aacgatcgat aactaaatga tagatgtgat ctggatcaca tacaagatat tttttataac
    19921 aatcattaat taatttaaaa atctttaaat ttcagttaaa tagaaagcaa ttatttttat
    19981 gggatctgga ctggtgaacg gaggtgatta tttttataat aacctttcat tcacagtcac
    20041 caggtacaac ggcatcatgg caactgactc aacacaatgt gtaaaaaaaa gccgtggccg
    20101 cccaaaagtg ttcgacaggg atgccgcgct tgataaggcc atgaaattgt tctggcaaca
    20161 cggttatgaa gcgacttctc ttgcggacct cgtcgaagcg accggagcca aagcgcccac
    20221 gctatacgcg gaatttacca acaaagaggg gttatttcgc gccgtactcg accgctatat
    20281 cgatcgtttt gccgctaagc atgaagcaca gctgttttgt gaagagaaaa gcgtggagtc
    20341 agcgctggct gactattttg ctgccatcgc caactgcttt accagcaaag acaccccggc
    20401 tggctgcttc atgatcaaca actgcaccac cctctcccca gattcaggag atatcgccaa
    20461 tacgctgaaa tcacgccatg cgatgcaaga gcgcactttg cagcagtttt tatgtcaacg
    20521 acaagcgcgc ggggaaatcc cgacccactg tgacgtgaca catctggcag aattccttaa
    20581 ttgtattatt caggggatgt cgatcagcgc acgcgaaggt gcatcgctgg aaaaactgat
    20641 gcagattgcc cgaacgactt tgcgtttatg gccggaactg gtgaaataag tcgcaaaaaa
    20701 agcccctcag cctgagcgaa tgaggggcta aattgcagag tacaaccttg ttatactttg
    20761 ttattcaaaa tcagttgccc gttgctatct agcgggatct gcgtacccgg atcgcggtcc
    20821 atgcggattt tgccctgctg atcgccaatc ttataggtca catcataacc gagcattttt
    20881 tctgacttgt catacaccgt tttacaacgc tgttgcgtag tcgtgtaagt atcgctttcc
    20941 tggagagagc cctggatctg gttaccggca tatccaccac ccagcgcccc cacaacagtg
    21001 gcgacatctt taccacgacc accaccaaac tgatgcccta tcacgccgcc agcaacagcg
    21061 ccgagcaccg acccggtaat gcgattttca tcctgcaccg gtcgacgatg ggtcactgtg
    21121 acgttgcgac actcctgacg cggtgtttta accgtttcct tgattggggt tgcagaaaca
    21181 acctgagcgt attgcgggcc acgttcaaac acgttcagac tggccactgc cgctacgccc
    21241 agcgcagctg cgacaccaat cccgataccc gccaacattg atttattcac gggaacacct
    21301 ccttctttgc agttatgcca atcatgcaag cgaaggagaa aagcagctat cagacagaag
    21361 atgaaaagcg tgggaatggc tggaaatact taaggattcg gaggagtctg aaggaatgcc
    21421 tgatgcgacg cttaacgcgt cttatcaggc ctaccgctcg taccatttgt ccagggcgga
    21481 tgaggcgttt atgccacatc cgccagtgta cgtcgattaa tgcaacttca aacgcggacg
    21541 gataacgcgg ttaatactcc ccaccagcat cattaatccg gttttaaagt aaccatgcag
    21601 cgcaatctga tgcattctgt atagcgagat gtacacaaag cgcgcaattc gtccttcaat
    21661 catcattgag ccgcgcgtca ggttacccat caggctaccg acggtggaga agttcgacag
    21721 cgataccagc gaaccgtgat ctttatactg ataatttttc agcggcttac cgttcatctg
    21781 cgccagaatg ttgttcattg cgcaagttgc catctggtgt gcagcctgag cgcgcggcgg
    21841 aacaaagccc ccttccggac gcgggcatga cgcgcaatcg ccaatagcgt aaatgtctgg
    21901 atcgcgggtg gtttgcagcg tcggttccac caccagctgg ttgatacggt tggtttcaag
    21961 accaccaata tctttcagga agtctggcgc tttgatcccg gctgcccaca ccatcagatc
    22021 agcctcaata tattcgccat ctttagtgtg caggccgcct tcatcagcac tggtgaccat
    22081 ggtttgcgtc agcacgcgaa cgccaagttt cgttagctcg ttgtgggccg cagcagagat
    22141 acgtggcggt aatgcaggca aaatacgttc acccgcttct accagcgtta cgttcagggc
    22201 ttcgttggtc aggcctttgt aaccgtagct gtgcagttgc ttgaccgcgt tgtgcagttc
    22261 agcggagagt tctacacccg tcgcgccgcc gccgacaatc gcaatgttca ctttgccatt
    22321 tgcgcccagg ttggcggagt atttcaggaa gagattcagc atctcctggt gaaagcgacg
    22381 cgcctggtgc gggttatcga ggaaaatgca gttctctttg acacctggcg tattgaaatc
    22441 gttagaggtg ctacccagcg ccattaccag ggtgtcatag gcgattttac gttccggaac
    22501 cagcagttca cctttctcat cgcgcagttc tgcaatagtg attgttttcg cttcacgatc
    22561 aatatcaatg acggaaccca gctggaactg gaaaccatga ttgcgcgcat gggccagata
    22621 gctcaacgca tcgacgcctt catcaagcga gccagtcgcc acttcgtgca gcagcggttt
    22681 ccacaggtgg ctgtggttac gatcgaccag cgtaattttg gcttttttct tgcgtcccag
    22741 cttatgcccc agctgtgttg ccatttccag cccaccagca ccgccgccga caatcacaat
    22801 ctttttcaat ggcgtagtca acgtgacccc cttaaattta ttaaccaatt gttaattaat
    22861 ggttattaac atagctctta attaacaaca ggttacagca atgaaaatgt tcttcgctat
    22921 tgagaataac atgaatggtg cattggtcat accaaaattg atgttaatca agttttgttg
    22981 ctgaaaagat aggcgacttt accgggtgag atcaaaaaac agagtttatt gtttattatt
    23041 tgagcgaatc tggaagggtg atatagggtg aagcgtatat cgagggcgtt tggtcgtact
    23101 gataagatgc gcagcatcgc atcaggcatt gatgctcgac tgtcggatgc ggcgtgaacg
    23161 ccttatccga cttacagaat ccggagcgca tcaggtaccg gcaaggttac ctgatgcgac
    23221 gagggcattt acccgagggt tttgaacgct ttaatgcgct gcaaatgcgg agagatattc
    23281 ttgaatttgt gcgtctgctc ttcgtcccag acaatctcgt aataatgatg caactcttca
    23341 gatgtccgct ggctgttaag cgcttcatca ttacgcgaca aaatcaccag acaacgatcg
    23401 cgattcttct cgcggaagtt ggtcacacac ttagtggcaa tatcggcata ctcttccggg
    23461 cgatcaatct tgccttccat gttctcataa gggaacaaat tagggttgaa gatcacctgg
    23521 cggatgtcgc aaagaaaacc aatccgttcc gcccagtatc cgcccaagcc aacgccgcaa
    23581 attagcggac gctcgtcaac gttcagttgc aacattttgt ccacttcttt aagcagatgc
    23641 tgcatatcat gtttcggatg ccgcgtactg tagcttatca agcgtacatc cgggtcaata
    23701 aactgcaatt gtaagacttt ctcgtggtta cccggactgt tagagtcaaa accgtgtaaa
    23761 tagataatca tcgcttcctc acataagcca gggttagtga cctgctttct cttcctgcca
    23821 gcgttcatgt aactgattca gacgggcgct gatcgctttc cagcgagccg agtccatcag
    23881 ttcctgtcgc gaaaatgaac ctttatgata caaacgtgta acacgttctg ccttgatcgg
    23941 tgacagatta tctaacacgc tgacggcccc tttacgatta ttgcagacca ggatcatatc
    24001 gcaacccgca tccagtgatg cctgcccgcg ttcggcataa ctgcccataa tcgcggcacc
    24061 ttccatcgat aaatcgtcag agaaaatcac gccgtcaaaa cccagttcct gacgcaaaac
    24121 ggttttcagc cagtagggag aaccgctcgc cggacgcgga tcaacatcac tgtagatcac
    24181 atgcgcaggc ataatggcgt cgagtttgtt ttcgcggatt aaggaactga agaccgacat
    24241 atctttagcg cgaatctccg cttgtggacg tggatcgcac ggtgtttctt tgtgtgagtc
    24301 tgccgttact gcaccgtgtc cagggaagtg tttcccggtc gttttcattc cggcttcatg
    24361 cataccatca ataaaccggc tggcaattgc cagggctttt tgtggatcgg catgataaga
    24421 acgctcgcca atcgccgcgc tgatatgccc gacatccagc acaggcgcaa agctgatatc
    24481 aatatccata gcgatcattt cgctggccat caaccaacct gcctcctgcg ccagtttgcc
    24541 accctcttcc attcctgaca gcgcagcgaa tgattgcgcc gctggcaagc gggtaaaacc
    24601 ttcacgaaaa cgctgcacgc gtccaccttc ctgatcaacc gccaccacca gatgattgcg
    24661 cgaagctgcg cggatctggc gcaccagttc acgtaactgg gcaggatcat gatagttacg
    24721 cgtaaagaga atcagccctc ccaccagcgg atgcgccagt atttcacgct cttccgcgtc
    24781 cagttcgtaa ccttcgacat ccaacattac tggacccaca ctgctctcct tattgtttta
    24841 ttaatagctg ccgccaggtg tcatcggcca gcctgataaa ttgttgatcg ccggtttgtc
    24901 gccagcggta ctcaaaccac cctgctttga gcatcagcag ccagggaaac catcgcctga
    24961 cctgacgcca taattgcgcc ggataaatct tcgcgcgagt ggcatagtca ttgaccaatt
    25021 gtcggtgctg ttcagcattt tccacccaca ccgccgccag ttccagcgcg atatcaccat
    25081 ctcctgcata ctcccagtcg atgagtttta accctgacgc gctatgcact aaatttccgg
    25141 cgtggacatc catatgcaat ggacttaagc gtaaaggccg tggttcccgc gctttgcgca
    25201 gacgttttaa cattcgcaac caacccactg tccgccgcgc cggatcgctt tgctgccagt
    25261 acagttccag taacggcaac aacgttattc gccagccaaa acgtggttgt tgatgtagat
    25321 aatacagcaa gcctgccagt tcgttggtat ccggcaaata cgtttttacc gcgccgggca
    25381 gatagtcgac taccatccag tcatggagat ataaatgcgg cttcggtgca atgcatgcgg
    25441 gtagttgtga taaagcccga tactggcgca agaacgctga ctgcggcgca tcaggatcgt
    25501 gcggctgacg cacaacaaaa cgctggccct ggtgttcaat gagaaaactc ccgccactaa
    25561 gcccactatt aaacgtcgtg acgggatgaa actgcgggaa aaagcgcgac agcaattcgt
    25621 cgcgcgtgat gggattattg ctgcgaaacg gcacctttac ctgaccagat aatttcgccc
    25681 gtctgcacca gcatcagctg catttgtagg gtcggagcgt taacgttgcc agaggcgctg
    25741 gagtacagca cgtaatgagc gccgacattg cgggcaatgc ctatggcttt actacgggtg
    25801 cctaaactgt cctgcggcga caaacctaac tgttgcttcg ccatcgacag ctgctgggcg
    25861 gaaaccaggg taaatttccc gttattagcc agcgcatttc gcagcgtttc ggtcgcttct
    25921 gcggcattca gcgaaccgtt agtacggttg ttaacgctat caaccagcag gacgctaccc
    25981 gcagtcaccc cgtcagcccc aagcatctta ctgaccatcg gctgcattgc gccattccag
    26041 tcataatggc gaatatgcgg cgcaggcggt gccgtttgat cttcgtgctc aattgggcct
    26101 ggctgctgcg ggatcgtcgg caccgagggc actgtgggga caggctgttg tggctcggct
    26161 ggttgttccg gcgctggttt cacttcttct accggtgcag gttcacgttg ccccacacac
    26221 ccggcgagaa acatcgccag cgcggtaatc aaggcgtagc gactcatttt tgtcatcaag
    26281 attcacccct tacaaatata gataaagtct gactttgtgc gcccccagaa aattggcgct
    26341 gccgtacagc gttaccgccg aatgtgcggg aatggtaacg ctgcgtggcc tttccagagg
    26401 atgcatctcc agccctctgg cgtcatacca gtaaaaacga taatgaacgg taacgggttc
    26461 ttgcctttcg ttataaagcg ttgaggatgc tgaaggttga atatcagacg tcgaaaggac
    26521 gggcttttct gcactgatgc ccgcagccaa taaagatgac tccatcacca gcgattgctc
    26581 atcatttacc ggaatttccg gatgtgaacg acagcccacc agcagtaaca acgccagaga
    26641 caccagccca aagcatcctt ttctcatcgt tacagacctt tatgcgccag cattggtccc
    26701 agcggacggc cacccaacaa gtgcatatgg atgtggtaaa cctcttgtcc gccatggcgg
    26761 ttggtattca tgatcagacg ataaccatct tcggcaatac cttcttgctc agcaattttt
    26821 gccgctacgg tgatcatacg tcccagcgcc tgctcatgct cagctgacac gtcgttcaca
    26881 gtcggaatga ggatattcgg aatgatcaga atatgcgttg gcgcttgcgg cgaaatatcg
    26941 cgaaacgccg ttaccagatc atcctggtag acgatatcgg aggggatctc acgacgaata
    27001 attttgctga atatagtttc ttctgccacg acattttcct ttttcaaata gccatgcgtt
    27061 gcgccatgct acgccgcgcc acactgcgag tatagagtat ggtcgagtta aacctgctct
    27121 ttcaacctta actcacgatt tcttaagcaa aaaatcgttt tgctgccgta tatatcgcca
    27181 ttattcccat ttctgcctgc gaatgcgctg aaaagacatt ctctgtttgg tttacacgtc
    27241 aaatatgaat gcgtatattt ctcatttgca tttacataca aaattattcg cacactaaat
    27301 ataaccgctc atcaccctgc tccggcatga gcctgacgac aacataaacc aagagatttc
    27361 aaatgctttc aacacaattt aacagggata atcaacatca agccatcacc aaaccgtcac
    27421 taattgccgg ttgcatagca ctggcactat taccttctgc cgcttttgct gcaccagcca
    27481 ctgaagaaac ggtgattgtt gagggttcag ccacagctcc agatgatggc gaaaatgatt
    27541 acagcgtaac gtctacctct gcgggtacca aaatgcagat gactcaacgt gatattcctc
    27601 agtcggtcac tattgttagc cagcagcgga tggaagatca gcagctacaa acgctgggcg
    27661 aagtgatgga aaacacgctg gggatcagca aaagtcaggc ggattccgat cgtgctcttt
    27721 attattcccg cggattccag atcgataact atatggttga tggtatccct acctattttg
    27781 aatcgcgctg gaatctgggc gacgcacttt ctgatatggc actttttgaa cgcgtagaag
    27841 tagtgcgtgg cgcgacagga ctcatgaccg ggacgggtaa tccatctgcg gcaattaata
    27901 tggttcgaaa acacgcgacc agtcgtgaat ttaaaggcga tgtctcggcg gaatacggta
    27961 gctggaacaa agaacggtat gtggcggatt tacaaagccc actcaccgaa gacggtaaaa
    28021 tccgcgcgcg aattgtcggc ggctaccaga ataacgactc atggctggac cgctacaata
    28081 gtgaaaagac cttcttctcc ggcattgtcg atgctgattt aggcgatctt acgacgcttt
    28141 cagccggtta cgaatatcag cgcattgatg ttaatagccc aacctggggc ggtttaccgc
    28201 gctggaatac tgatggcagc agcaacagtt acgatcgtgc acgcagtacc gcacctgact
    28261 gggcgtacaa cgataaagag atcaacaagg tctttatgac cctgaagcag cggtttgctg
    28321 atacctggca aacgacgctg aatgccaccc actccgaagt cgaatttgac agcaaaatga
    28381 tgtatgtcga tgcctatata aacaaagcgg atggtatgct ggttgggcca tacagtaatt
    28441 atggacctgg ttttgattat gtcggcggca ccggttggaa cagcggcaaa cgtaaagttg
    28501 atgcgctgga tttgttcgct gacggtcgtt atgaattgtt tggtcgtcag cacaatctaa
    28561 tgtttggtgg cagttacagc aaacaaaaca atcgttactt cagttcatgg gccaatattt
    28621 tcccggatga aattggcagt ttctacaact ttaatggcaa tttcccacaa accgactggt
    28681 caccacagag cctggcgcag gacgatacca cacatatgaa atcgttatat gctgccactc
    28741 gtgtcaccct tgccgatccg ctgcatctga tcctcggcgc acgttatacc aactggcggg
    28801 ttgatacgct gacttacagc atggagaaaa accacaccac gccttatgct ggtctagtgt
    28861 ttgacatcaa tgacaactgg tcgacctacg ccagctatac ctctattttc cagccgcaaa
    28921 atgatcgtga cagttcaggc aaatatctgg ctccaatcac cggtaacaac tacgagctgg
    28981 gtctgaaatc ggactggatg aatagccgtc tgaccaccac gttagccatc ttccgtattg
    29041 agcaggataa tgtcgctcag tccaccggta cacctatccc cggcagcaac ggcgaaaccg
    29101 cctataaagc ggtggatggg acagtcagta aaggggtgga atttgaactc aacggcgcaa
    29161 ttaccgacaa ctggcagctg acatttggcg caacgcgcta tattgcagag gataacgaag
    29221 gaaacgccgt taatcctaat ctgccacgca ccacggttaa aatgttcacc agctatcggt
    29281 tgcctgtcat gccagagttg acagtcggcg gtggtgttaa ctggcaaaat cgcgtgtata
    29341 ccgacaccgt gacaccgtat ggcaccttcc gcgccgagca aggtagctac gcgctggtgg
    29401 atctcttcac ccgctaccag gtgacgaaaa acttctcgtt acaggggaac gtcaataacc
    29461 tgttcgacaa aacctacgat accaacgtgg aaggttctat cgtctacggc acaccgcgta
    29521 atttcagcat taccggcacg tatcaattct gaataacacc tgtaaaaaag gcagccatct
    29581 ggctgcctta gtctccccaa cgtcttacgg attagtggtt acggatgtac tcatccatct
    29641 cggttttcag gttatcggat ttagtaccga aaatcgcctg aacaccagaa ccagcaacca
    29701 ctacgcccgc tgcgcccagt ttcttcaggc cggcctgatc cactttagac acatcagcaa
    29761 cgctgacgcg cagacgggta atacatgcgt cgaggttagt aatgttttct ttaccaccaa
    29821 atgcagcaac cagagccggt gccatttcgc tggtacctgt cgcttttgca tcttcagtcg
    29881 cgtcttcacg acccggcgtt ttcagatcca gtgctttaat cagcacgcgg aagatggtgt
    29941 agtaaacaat cgcataaccg ataccgacga tcgggaacag ccacagtttg ctgctgttac
    30001 cagacagaac gatgaagtcg atcagaccgt gcgagaacga cgtaccgtca cgcatcccca
    30061 gaagaataca gattgggaat gccaggcctg ccagaatcgc gtggatgatg tacaggatcg
    30121 gcgcaacgaa catgaaggag aactcgatcg gctcggtgat accggtcagg aacgaggtca
    30181 gcgccgcgga gatcataata ccgcccactt tcgcgcggtt ttctggttta gcagagtgcc
    30241 agatagcaat tgcggcagct ggcagaccgt acattttgaa caggaagcca ccagacagtt
    30301 tacccgcagt cgggtcaccc gccatataac gcggaatgtc gccgtggaaa acctgacctg
    30361 ctgcgttggt gtattcacca atctgcatct ggaaaggtac gttccagatg tggtgcagac
    30421 caaacggtac caggcaacgt tcgatgaaac cgtaaatgcc aaacgcaact accgggttct
    30481 ggtaagcagc ccactgagag aaggtctgga ttgcagaacc aatcggcggc caaatgaagg
    30541 acagcacaac gccagtaaag atggcagcca ggccagaaat gatcggcaca aagcgtttac
    30601 cggcaaagaa gccaagatac tcaggcagct taatacggta gaaacggtta aacatgtacg
    30661 ctgcgatcgc accggagata atccctccga gtacgccagt atccgccagg tgtttagagg
    30721 cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac cacggccatg gttttaacca
    30781 tgatgccata ggcaacaact gcggccagcg cggatacgcc atcgttattg gtaaagccga
    30841 gggcgacacc gatcgcaaaa atcagtggca tgtttgcaaa gacggaaccg cctgcttctg
    30901 ccataacatg cgatacaacg gcgggcagcc agctgaaatt cgcggaaccg acgcccagca
    30961 gaatacctgc gataggcagt acggataccg gcagcatcag cgatttaccg accttttgca
    31021 ggttagcaaa tgcattctta aacataattg agagtgctcc tgagtatggg tgcttttttt
    31081 acgttctcac gcgtggcaag gggggagagc ctcgccgtgt acagggcatc taagcgccct
    31141 ttatttatta cacagagtaa aataattcag tgccaatttg tttgacggct atcacgtttc
    31201 aacttcagat cattggagaa gttgcacatt ttgacaaaac atatgtcaca atatttcaaa
    31261 cgtggttatt caccgcccaa ttttcggcgg tgaactttac tcgttttagc cattaattac
    31321 gagctttaaa aaaactcatt caacggatgg attgaaggcg ggaagcgtcg atgtgaaaca
    31381 gacgggcgaa gttatcggtg gttacctgcg ccagttcttc aacggcaaca cctttcaaca
    31441 cagccatgta ttctgcaacg tcacgaacca tcgcaggttg attctctttt cctcgatgcg
    31501 gtaccggcgc aaggtaaggt gagtcagttt ccaccagtaa ccgatccagg gggacataac
    31561 gcgcagcatc gcgcagttgc tccgcattac ggaaggtcac aatgccggaa aaggagatgt
    31621 aaaatccgag atccagtaat ttacccgccg tttctctgtc ctctgtaaaa cagtgtagta
    31681 cgccaccgca atccgtcact ttttcttcgc gcagaattgc cagcgtatcg gcacgggcgt
    31741 cacgggtatg gacgataacc ggcttgttca gttcacgacc aatctggata tgatggatga
    31801 aggactcttg ctgacgtact ttagtttccg gtgtgtaata ataatccagc ccggtttcac
    31861 ccagcgctac aacaccctct tctgccgcca gacggcgtaa atcttctaca tcgtagggat
    31921 cattctggtt gagtggatgc acgccacaag aaaatacgac gttgtcacgt tcgcctacca
    31981 gatcccgcat atgtaaataa cccggtaatg ttgtggcgac tgccagacaa aatttcacat
    32041 cgcgtgcggc ggctttcgcc agaacgtcat ccacgtcctt atgcaaagat tcataatcca
    32101 gaccatcgag atggcagtgt gagtcgacta aaaacatgat gtctctctta aagatgagga
    32161 accggtagca caacgcccgg ttgcaggtaa tgctcaatac gcagtaaaag atcggtgatg
    32221 agaagctcgc ggttgatgcc tgtgacagac attaactgtt cacgaatgtg gcaaacatcc
    32281 cccagtatag cctgcaggcg cgagggagaa agatggtttg ccagttcgac gaccaggccc
    32341 ggcacatcaa cattggtcac ctgcgcagcg ccatgatggc gttttagcgc atccatcagc
    32401 aacgttgcca gccagtgtaa acgcgccgga gcttgttcat gattaagggc cgctagcagc
    32461 gaataccagt cgcccgatgg cacgctatat gccaacgcct gacacaatgt ttcacgagcc
    32521 tgccagttat ctccctgaaa caacgccagt gccgcgccag gcgaaccggc gcttaaacgc
    32581 aatgcggcaa gtaatgcatc ctgtgacatt gtcacttcgc gtgaaagcca ggtcacggcg
    32641 tactgttccg gcggcggcgc aaggtaatgt aaccgacaac gactacgtaa tgttgccagt
    32701 aaacgttcag gctcgcgggt agccaggaaa aaccaggttt ctgctggtgg ctcttcaagc
    32761 gttttcagca atgcgttagc cgcggcgtcg gtcagtaagg cagcatcggt tacccagaca
    32821 actttcgcac cacctaagcg tgcgtgctca ttcagctttt cggtgatctc acgtaccgca
    32881 tcaatgccca gcgtattttt ccctttttcg ggagccaggg tgttgtaatc gggatgcgtg
    32941 ccagcctgca tcaactgaca tccacgacag tgaccgcaac ttttgtggcc ctgcggttgt
    33001 tggcagagta aataacggct cagggcgtag attaaagcat catcgcccat gcccggtaac
    33061 gcctgaatga gtagcgcatg gtgacctctt ccggcctgat agctggctac cagtttttcg
    33121 aaatcaggtc gtaaccatgg ataccatctc atgcgtccaa ctccttcacc cagtgggtca
    33181 cggtagtgcg gattgcatcc atcacggcct ccagcggctg ggtggcatca atggtatgaa
    33241 tactgttatc ttgtgcagcc agttccagat agcgggagcg ggtgcgatta aagaaatcga
    33301 aagactcttg ctcaatacga tccagctcgc cgcgcgcacg cgcgcgtttt aagccaactt
    33361 ccggggtaac atcgagatag agcgttaagt cggggcgaaa atccccgaga acagcatcac
    33421 gcagtgttgc cagcatatgt tggtcaatac cacgtccgcc gccctgatac gcctgagtgg
    33481 agagatcgtg gcgatcgcca atcacccagg tgccgttagc cagcgcaggt ttgatgaccg
    33541 tttctaccag ttgaacgcgc gcggcataaa acatcagaac ttcggcttta tcggtaatga
    33601 cttcatcgcc taccgatttg atatccagca ccaggcttct taacttttcg gcaagttgcg
    33661 taccgccagg ttcccgcgtg aaaaccatgt cgcggatacc cagttgctca agcgtctcaa
    33721 ccaccacatt acgcgcggta gttttgcctg cgccttccag cccctcaatg acgatatact
    33781 tactgcgcat ttttttcctt aagcactttc agataatcct gcacagactt gttatgactt
    33841 gcaagattgg tattaaacgt gtgaccacct ttaccatcgg ccacaaaata gagatacggc
    33901 gtttttgccg gatgcgcagc agccttcagc gaatccgccc ccggcgtagc tatcgcaccc
    33961 ggcggcaaac cggtaatggt ataggtgtta tacgctgtcg gcgtttccag gtctgcacga
    34021 gaaagtttgc cattataacg ctctcccatc ccgtaaatca cggttgggtc ggtctgcagg
    34081 cgcataccaa tgcgtaaacg gttgataaat accgaggcaa cctgatcgcg ttcactggca
    34141 acggcggttt ctttttcgat aattgatgcc atcgtcacca gctgattttt atctttataa
    34201 ggcagaccgt ccgcacgccc ttcccaggcg ctatcgaccg ctttcaccat tttcttgtgc
    34261 gctcgcttga gtaacgcgac atcggtggta ttggcggtat acatccaggt gtctggccag
    34321 aaccaacctt caatccactc cgggttttcc agttcaagtg cctgcgctac ggtggcgtac
    34381 ttatcgtcac tcagcgtgtg cttgatatac ggagcctcgc gcaattgctt gaggtagtcg
    34441 ctcagacgca tcccttctac cagtcgcagc gggaactgtg cttctttacc gctttccaac
    34501 aatttcagca tctcgcgcac ggtcatctgc ggtgtaaagc ggtaagtccc ggctttaaag
    34561 tgagaaagat ccggttcgat acgcagcaac cattgaaaaa cccgtgggcg attgatgatc
    34621 ttatcggcat aaagctgttc accaagcgcc agacgtccgg tccctggctt cagggtaaat
    34681 atcgtctctt ctttgataag caatttgctg tcggcaagat ggcgaacctt ccagacgccc
    34741 acaccagcgg cgatacccag taccaccagc aataacaaga taatcaataa cacttttttc
    34801 atgactaatt cgggcgctca caaagtgggg ctaaatattc atataacgtt gctgacgaaa
    34861 aggagacatc gccacaggca cgtacgggca tcactggcat taacgcatta caaataacca
    34921 tctcatctgc ctgcaacgcc tcttccagag aggcttgcac ttcgacaagc tgataagagg
    34981 attgtgccag taaacggata cagaattgtc gcataatgcc gttaacacct gcctgatcca
    35041 gtcgcggcgt ataaactacg ttgcccttcc gccagaacaa attagccgca cagcattccg
    35101 taacccaccc ttcgctgtca aggaccagcg cctcatcagc gtttgtctgc tcaagatgag
    35161 agcgaatcaa tacttgctca agacgattga gatgtttaat acctgcaaga tgaggattgc
    35221 gccccagccg caccgggctt agcgccaacg tcatcccctc gttacgcaaa cggtcgtaat
    35281 gtgcaggata agccgtaacg gagagaatcc gcgttgccgg tccactgttc aatgtgctgt
    35341 accctcgccc gccactaccg cgactgatca cgactttcag tacaccattt tgctgttctg
    35401 ctgccagcgt tttcatctct tgttcaagct gtggccagaa gtcacaggaa atcatcaacc
    35461 gctgacaagc atcctgtagt cgctggatat gcgccgataa caaactgact ttaccgtcga
    35521 taactctggc ggtggtaaaa caaccatcac caaactgcgt tgcccgatcg cttactgcca
    35581 gcgattcctg cttataaccg ttaattaaga acataccggc tccttatcag agaattgtta
    35641 gtgtggcagc atgttcacta cggaacaagt cggaataaaa gctaagaaaa aaggcccgca
    35701 agcggacctt ttatatgggt ggaaaatgac aacttagatc tttttaaaga tcaaagaacc
    35761 attagtgcca ccgaagccga aggagttaca cagcgtgtat tccattccgc tgacctgacg
    35821 tgcttcatgc ggtacgaaat ccagatcgca accttcatcc gggttatcca ggttgatggt
    35881 tggcggaaca gcctgatcgc gcagcgccag gatggagtag atagattcta ctgcacccgc
    35941 cgcacctaac aggtgaccgg tcatagattt cgtggagctt accaacacac ggcttgcagc
    36001 ttcaccgaag atggttttca ccgcctgcgc ttcagcttta tcgccagccg gcgtagaagt
    36061 accgtgcgcg ttaacgtagc caatctgact cgcttcaatg cctgcatcac gcagagcatt
    36121 tgccattgcc agagctgcgc ctgcgccatt ttctggcggt gacgtcatat gataagcatc
    36181 gctgctcata ccaaagccga cgagttcagc gtaaattttc gcaccgcgtt ttttcgcgtg
    36241 ttcgtactct tcaagtacca gcataccggc accatcgccc agtacgaagc catcacgctc
    36301 tttatcccac gggcggctcg ccgcttgcgg gttatcattg cgggtagata atgcacgtgc
    36361 cgcgccaaaa ccaccaacgc ccagcggcgt actggctttc tctgcgccac ctgcaaccat
    36421 cacgtcagca tcgccatacg cgataatacg cgcagcatgg ccaatgttgt gcacgccgga
    36481 agtacaggcg gtcgcgatag agatgcttgg gccacgcagg ccatacatga tagtcagatg
    36541 acctgccacc atgttcacaa tcgttgacgg aacgaagaat gggctgatct tacgtggacc
    36601 accgttcatc agagatgtgt ggttttcttc gatcagtccg aggccgccaa tcccggagcc
    36661 aattgcggca ccaatgcggg ttgcgttctc ttccgttatt tcaaggccag aatcctgcat
    36721 ggcctgaacg ccagcgacaa ttccatattg aatgaaggca tccatcttgc gctgttcttt
    36781 gcgcgagata atgtcctcac agttaaaatc ctttactaag ccagcaaatt tcgttgcata
    36841 ggcgctagta tcgaaatggt cgattaggct gatgccactc tgaccggcaa gcagagcttt
    36901 ccaggtagac tctacggtat tgccgacagg agacaacatg cccagtccgg tcacaactac
    36961 acgacgctta gacacgtttg tcctccaggg agggaaaaaa tgattctagt gggacaaaaa
    37021 gataaaactc aggcggtcga acgaccgcct ggagatgttc acttacgcct ggtggccgtt
    37081 gatgtaatca atggcagcct gaacggtggt gattttctca gcttcttcgt ccggaatctc
    37141 agtatcaaac tcttcttcca gagccattac cagctcaacg gtgtcaagag aatccgcgcc
    37201 caggtcttca acgaaagaag cattgttggt aacttcttcc tgcttaacgc ccagctgttc
    37261 gccgataatt ttcttaacgc gttcttcgat agtgctcata ctcttaaatt tcctatcaaa
    37321 actcgctttc gcgatggttt tcgtagtgta taaaatgttg aaaaatttgc aactaaatcc
    37381 cggcaggtct taccacgatt ttacgttatt ttgaggcctt accccccaat aacgcaaatc
    37441 attttgcact aattattact cataaccacg cagactgcgc gcaacatgag cattttgtgc
    37501 aaatcgcggt cagaccatgt acatcccgcc gttcacatgc aaagtttcac ccgtgatgta
    37561 agctgcttcg tcggatgcca ggaatgcaac cgcgttggcg atttcctgtg cgccgccgag
    37621 gcgacccgca ggaacctgcg ccaggatacc cgcacgctgg tcatcgctca gcgcacgtgt
    37681 catgtccgtt tcaataaagc ccggagcaac aacgtttaca gtaataccgc gtgacgcaac
    37741 ttcgcgcgcc agtgatttac tgaagccgat caagcccgct ttcgccgcag cgtagttggc
    37801 ctgaccgcca tttcccatgg taccaaccac agaaccgata gtgataatac gaccatgacg
    37861 ctttttcatc atagcgcgca ttaccgcttt tgacagacgg aaaacagatg aaaggttggt
    37921 ttcgataata tcgttccact cttcatcttt cattcgcatt aacaggttat cacgagtgat
    37981 accggcatta ttgaccagga tatccacttc accaaattct gcgcgaattt tttccagaac
    38041 agattcgata gatgccgggt cggtcacatt caacatcaga cctttgccgt tagcacctaa
    38101 ataatcactg atcgcctgag cgccattttc actggtcgca gtgccaataa ctttcgcgcc
    38161 acgggctgcg agcgtttcag caattgcgcg gccaattccg cggcttgcac cggttaccag
    38221 tgcgattttt ccttcaaaat tcataatttt cctcttttaa agctcgagcg ccgctgccat
    38281 cgctgaaggt tcgttcagcg ccgaggcggt cagggtgtcg acaatgcgtt tcgtcaggcc
    38341 agtaagcact ttgcccgggc cgacttcata gagatgttct acgccttgcg ctgccatgta
    38401 ctcaacagac ttcgtccact gaaccgggtt atacaactga cgtaccagtg cgtcacggat
    38461 ggcatcacca ttggtttcgc atttcacatc aacgttattc acaacaggaa ctgttggtgc
    38521 gttaaaggtg attttcgcta attctactgc cagtttgtcg gctgctggtt tcatcagcgc
    38581 acagtgagat ggtacgctca ctggtaacgg cagagcgcgt tttgcgcccg cagctttaca
    38641 ggcagcgcca gcacgctcaa ccgcttcttt atgaccggca ataaccacct gtcccggaga
    38701 gttaaagttt accggagaaa cgacctgacc ttctgcagct tcttcacacg ctttcgcaat
    38761 agacgcatca tccagaccga tgattgccgc catagcgccc gttccttccg gtacggcttc
    38821 ttgcatgaac ttgccgcgca tctcaaccag acgcaccgca tcagcgaaat caatcacacc
    38881 agcgcaaacc agcgcggagt attcccccag gctgtgaccg gccatcattg ccggtgcttt
    38941 accgccctgc tgctgccata cgcggtacag cgcaacagag gcagtcaaca gcgctggctg
    39001 ggtttgccag gttttattca gttcttcagc tggcccctgc tgggtcatcg cccacaggtc
    39061 gtagcccagc gccgcagaag cttctgcaaa cgtttcttcg acaattggat agctcgccgc
    39121 catatcagcc agcattccaa cggtttgaga accctgtcca gggaacacaa atgcaaattg
    39181 cgtcatgttt taatccttat cctagaaacg aaccagcgcg gagccccagg tgaatccacc
    39241 gccaaaggct tcaagcagaa ccaactgccc cggcttaatg cgcccgtcgc gtacagcttc
    39301 atccagcgcg cacgggacag aggccgcaga ggtattaccg tggcgatcca gcgtcaccac
    39361 gacattgtcc atcgacatac cgagtttttt cgccgttgca ctgataatac gcaggttagc
    39421 ctgatgcgga accagccagt ccagttgaga acggtcaaga ttattcgccg ccagcgtctc
    39481 atcaacgatg tgcgccagtt ccgttaccgc aaccttgaag acttcgttgc ccgccatcgt
    39541 cagatgaatt gaattctctg gattcacgcg gtcggcgttt ggcagcgtca gcaattcacc
    39601 ataactaccg tcggcatgca gatgggtgga gatgattccc ggctcttcag aggcagccag
    39661 caccgcagcg cccgcgccat cgccaaaaat aataatagtc ccacgatctg ttggatcgca
    39721 ggtgcgcgcc agtacatcgg aaccgacgac cagagcatac ttcaccgccc cagatttcac
    39781 gtattgatcg gctacgctta atgcataggt gaaacctgcg caggctgctg caacgtcaaa
    39841 tgccgggcaa cctttaatgc ccaacatgct ttgaatctga caagctgcgc tcgggaaagc
    39901 gtgcgtagca gaagtcgttg ccacaacgat caggccaatc tggtctttct caatgcccgc
    39961 catctcaatt gcgcgtgtcg ccgcttcaaa gcccatggtt gaaacggttt cgtttggcgc
    40021 ggcaatgtgg cgttcgcgga taccggtacg agtgacaatc cactcgtcag aggtgtccac
    40081 cattttttcc aaatcggcgt ttgtccgcac ttgttcgggc agatagctgc cagtaccaat
    40141 aatcttcgta tacatgtacg ctcagtcact tttcggttat ataccgtcac ttgcaaactg
    40201 cgagttcgct ggcagcgtcc tgctaccgca gagttccgct tttgccaccg tccagcagct
    40261 caaaaccagc tgggtataca gattccaggc gagcggcaat tcgctgagga acttgtcgct
    40321 gcaccgcctg cactgcctgt tcaatcgcga ccgcaaaagc tcgctgattg gctgcaccat
    40381 gactttttat caccgtgccg cgcaatccta acagacaggc gccgttatac tggtcggggt
    40441 tgaggtgact gaatcgcctc gtcaggctct tttgtagcca acgctttaat aacagtagcc
    40501 accacgaccg ttttttccct tcaccctgag atttcagcag agaaaggaac atcctgacaa
    40561 caccttccat cgtctttaat gtgacatttc ctgtaaagcc gtcacaaacc agcacatctg
    40621 tcttgccagt taacaactca ttggcttcaa gatagccgat ataattgata gaagggattg
    40681 ttttaagcac cgctgaggca tcccgaatac tgtcgagacc ctttacttct tcttcaccaa
    40741 tattgagcaa cgccacgcga ggattgggaa tttccaccac ctcttcagcc agaactgagc
    40801 ccataatggc aaattgcacc aacattgtgc tgtcacaatc gacgttggcc cctaaatcga
    40861 ggaccaccgt tttgcccttt tgctgatgcg gtaataccgt caccagcgcc ggacgctcaa
    40921 tcccctccag gggcttgagt aataattttg ccagccccat cagtgccccg gtattaccgg
    40981 cactgacaca ggcttgcgct cgaccttctt tcaccagctc cagggccacg cgcattgaac
    41041 tcccacgact ggcgcggata gcttgcgaag gccgggcatc actggcgata actgactgcg
    41101 caggaataat ctgcagacgc gaacgttgtt caaagtcagc tttagcaagt aatggcgtga
    41161 tggcgtcggg attgccgact aaaagaagag tgagttgcga attagaattc agtgcctgca
    41221 atgctgcagg cactgtcacg gaagggccaa aatcccctcc catgacatct aacgccaggg
    41281 ttagacgtgt caaggtatcg tcgccgcccg gtttggtctt tccccagttg cctggggaaa
    41341 atcctcacta agcttcatca cgcagacgcg tgattactta gcgatgacct tgcggccgcg
    41401 gtagtaaccg tcggcagtga tgtggtgacg caggtgtttt tcaccagaag ttttgtctac
    41461 agacaggctg gtgactgcgg tcagcgcgtc atgggaacga cgcatgccac gtttggaacg
    41521 ggttggttta ttctgttgta cggccatgga ccttactcct caattacggt ttatccccga
    41581 tccaacgggg agcaccaatt acttacgctt taagctggct aatacggcaa atgggtttgg
    41641 cttttgcgct tcttcaggca gttcaccaaa gaccatgtcc gcttcggaca cttcacagtg
    41701 ttcagaatca tgcaccggaa ctaccggcaa ggcgaggatg atttcatctt caaccattgc
    41761 aagcagatcg atttcaccga attcgttaac ctcaatcggt tcatacgctt ccggcagtgc
    41821 ttcagcctgt tcgtctgaac gcacaggact aaaacaatac gttgtgtaga cctgatgagt
    41881 aaacggcttc ccgcaacgct gacactcgag cgttaccgtc accttcgcat cgccgtttaa
    41941 cactgcgaga cgttggttat cgatagcgaa cgacatggag cattccacat cactgtccac
    42001 actgactacg gattcggcga cgcgctcaac ctgatcaggg gtatagatac cctggtaatc
    42061 aaggcgtttt tgagccgtac gaaccggatc gagagtcagg ggtaatttta ccttttgcat
    42121 agggcgcgca tattaacttt gtaacgtcat agagtcaaag aaaaaggcag cctgtggttg
    42181 ccttttgcca ataattcgca cacattgcgg gttactgctt tattttcgtt cagtgctgat
    42241 aacgaacatc aacctgcaaa acgaagcgca tagtttaaaa tggctatcat caatacgcta
    42301 tatctgatgg aaaaaaatat gcctaaactt attttagcct ccacctcgcc ctggcgtcgc
    42361 gctcttctgg aaaaactgca aatctctttt gaatgtgcag cacctgaggt cgacgaaacc
    42421 ccacgcagcg acgaatcacc gcgacagttg gtgcttcgac tggcacaaga aaaagcgcaa
    42481 tctctggcgt cacgttatcc ggatcattta attattggtt cagaccaggt atgtgttctt
    42541 gatggcgaaa tcaccggtaa accgttaacg gaagaaaatg cccgtctgca attacgcaaa
    42601 gccagcggca atatcgtcac cttctatact ggactggcgt tgtttaattc ggcgaatggg
    42661 catctgcaaa cagaagtgga gccttttgac gtccatttcc gtcacctgag cgaggcggag
    42721 attgataatt acgtgcgtaa ggagcatccc ctgcactgcg cgggtagctt taagagtgaa
    42781 ggatttggca ttacgctgtt tgagcgttta gaggggcgtg accctaacac gctggttggt
    42841 ttaccgctaa tcgcgctgtg tcagatgtta cgtcgggaag ggaaaaatcc gctgatggga
    42901 tagttttttg cgccagatgc ggttacgtcg catccggcaa tcggtgctgg cggcctgatg
    42961 cggcgtaaac gccttatcag gccaaccgcc cattatcagg cttatatgct tgtattagcg
    43021 cgcgttacgc agcttttgca aacaacgctt caaaccatca tccatcggcg cttcgatacg
    43081 catcacctca ccggtccccg gatgggtaaa cttcaacgct gcggcgtgca ggaacagacg
    43141 atttaatccc gtgcctgctt cagtgagctg tctgtcaaat tcacggtcac cgtagcgatc
    43201 gtcaaaggca atcggatgac ccgcatactg tgtatgcaca cggatctgat gagtacgtcc
    43261 ggttaccggg ctacagcgca ccagggtggc aaatgcatag cgttcttcca ctttaaagcg
    43321 tgtttccgac ggtttgcctt cctgactcac acgcacgata cgttcgccgc tttgcagaat
    43381 atttttcagt aacggcgctt gaacgctctt cacatgcgac tgccactgac cgcgcaccag
    43441 cgccaggtaa tctttttgca tccctttttc acgtaattgc tcatgcagag aacgcaacgc
    43501 cgagcgtttt ttcgctacca gcaatacacc tgaagtgtcc cggtcgagac gatgaaccag
    43561 ttcaaggaac cgcgcttccg ggcgcaacgc ccgcaaacct tcgataacgc cgaagcttaa
    43621 accactgccg ccatgtaccg ccgtaccgga aggtttattc agcaccagga tatgatcatc
    43681 ttcatataag atgacgtccg ccagcgccgc caccttttgc agatgtggtg aaaccgcctc
    43741 ttcttcccgc tcagcaacgc gaaccggtgg gatacgcacc tcatcacccg cttcgagttt
    43801 atattcaggc ttaatacgtt ttttgttcac ccgcacttcg cctttacgca aaatacggta
    43861 aatcatactt tttggtacgc ctttcaattg ggtacgcaaa aagttatcga tacgttgccc
    43921 cgcttcgtca gcggtgatag caacaatttt tacggatgga gtctctgttt tcatggttgg
    43981 cgattctaat tagccaacag gattcgcgcc actcattttt ctatgcttat atttactttt
    44041 gcaccttatt acttcactgc gtgctcactt tattgatggt tattaaacca atcaccagca
    44101 agaagtgaaa aaactgtgag taagcgggtg ataaatggta aaagtcatct tgctataaca
    44161 aggcttgcag tggaataatg aggccgtttc cgtgtccatc cttgttaaaa caagaaattt
    44221 tacggaataa cccattttgc ccgaccgatc atccacgcag caatggcgta agacgtattg
    44281 atctttcagg cagttagcgg gctgcgggtt gcagtcctta ccggtagatg gaaatatttc
    44341 tggagagtaa tacccagtct gtttctttga taattgcgct gtttttccgc atgaaaaacg
    44401 ggcaaccgac actctgcgcc tctttgagct gacgataacc gtgaggttgg cgacgcgact
    44461 agacacgagg ccatcggttc acacccggaa aggcgttact ttgcccgcag cttagtcgtc
    44521 aatgtaagaa taatgagtaa gttacgatga aaagaatgtt aatcaacgca actcagcagg
    44581 aagagttgcg cgttgccctt gtagatgggc agcgtctgta tgacctggat atcgaaagtc
    44641 cagggcacga gcagaaaaag gcaaacatct acaaaggtaa aatcacccgc attgaaccga
    44701 gtctggaagc tgcttttgtt gattacggcg ctgaacgtca cggtttcctc ccactaaaag
    44761 aaattgcccg cgaatatttc cctgctaact acagtgctca tggtcgtccc aacattaaag
    44821 atgtgttgcg tgaaggtcag gaggtcattg ttcagatcga taaagaagag cgcggcaaca
    44881 aaggcgcggc attaaccacc tttatcagtc tggcgggtag ctatctggtt ctgatgccga
    44941 acaacccgcg cgcgggtggc atttctcgcc gtatcgaagg cgacgaccgt accgaattaa
    45001 aagaagcact ggcaagcctt gaactgccgg aaggcatggg gcttatcgtg cgcaccgctg
    45061 gcgtcggcaa atctgctgag gcgctgcaat gggatttaag cttccgtctg aaacactggg
    45121 aagccatcaa aaaagccgct gaaagccgcc cggccccgtt cctgattcat caggagagca
    45181 acgtaatcgt tcgcgcattc cgcgattact tacgtcagga catcggcgaa atccttatcg
    45241 ataacccgaa agtgctcgaa ctggcacgtc agcatatcgc tgcattaggt cgcccggatt
    45301 tcagcagcaa aatcaaactg tacaccggcg agatcccgct gttcagccac taccagatcg
    45361 agtcgcagat cgagtccgcc ttccagcgtg aagtccgtct gccatccggt ggttcgattg
    45421 ttatcgacag caccgaagcg ttaacagcca tcgacatcaa ctccgcacgc gcgacccgcg
    45481 gcggcgatat cgaagaaact gcgtttaaca ccaacctcga agccgccgat gagattgctc
    45541 gtcagctgcg cctgcgtgac ctaggcggcc tgattgttat cgacttcatc gacatgacgc
    45601 ctgtacgcca ccagcgtgcg gtagaaaacc gtctgcgtga agcggtgcgt caggaccgtg
    45661 cgcgtattca aatcagccat atttctcgct ttggcctgct ggaaatgtcc cgtcagcgcc
    45721 tgagcccatc actgggtgaa tccagtcatc acgtttgtcc gcgttgttct ggtactggta
    45781 ccgtgcgtga caacgaatcg ctgtcgctct ctattctgcg tctgatcgaa gaagaagcgc
    45841 tgaaagagaa cacccaggaa gttcacgcca ttgttcctgt gccaatcgct tcttacctgc
    45901 tgaatgaaaa acgttctgcg gtaaatgcca ttgaaactcg tcaggacggt gtgcgctgtg
    45961 taattgtgcc aaacgatcag atggaaaccc cgcactacca cgtgctgcgc gtgcgtaaag
    46021 gggaagaaac cccaacctta agctacatgc tgccgaagct gcatgaagaa gcgatggcgc
    46081 tgccgtctga agaagagttc gctgaacgta agcgtccgga acaacctgcg ctggcaacct
    46141 ttgccatgcc ggatgtgccg ccagcgccaa cgccagctga acctgccgcg cctgttgtag
    46201 ccccagcacc gaaagctgca ccggcaacac cagcaactcc tgcacaacct gggctgttga
    46261 gccgcttctt cggcgcactg aaagcgctgt tcagcggtgg tgaagaaacc aaaccgaccg
    46321 agcaaccagc accgaaagca gaagcgaaac cggaacgtca acaggatcgt cgcaagcctc
    46381 gtcagaacaa ccgccgtgac cgtaatgagc gccgcgacac ccgtagtgaa cgtactgaag
    46441 gcagcgataa tcgcgaagaa aaccgtcgta atcgtcgcca ggcacagcag cagactgccg
    46501 agacgcgtga gagccgtcag caggctgagg taacggaaaa agcacgtacc accgacgagc
    46561 aacaagcgcc gcgtcgtgaa cgtagccgcc gccgtaacga tgataaacgt cagacgcaac
    46621 aagaagcgaa ggcgctgaat gttgaagagc aatctgttca ggaaaccgaa caggaagaac
    46681 gtgtacgtcc ggttcagccg cgtcgtaaac agcgtcagct caatcagaaa gtgcgttacg
    46741 agcaaagcgt agccgaagaa gcggtagttg caccggtggt tgaagaaact gccgctgccg
    46801 aaccaattgt tcaggaagcg ccagctccac gcacagaact ggtgaaagtc ccgctaccag
    46861 tcgtagcgca aactgcacca gaacagcaag aagagaacaa tgctgataac cgtgacaacg
    46921 gtggcatgcc gcgtcgttct cgccgctcgc ctcgtcacct gcgcgtgagt ggtcagcgtc
    46981 gtcgtcgcta tcgtgacgag cgttatccaa cccaatcgcc aatgccgttg accgtagcgt
    47041 gcgcgtctcc ggaactggcc tctggcaaag tctggatccg ctatccgatt gtacgtccac
    47101 aagatgtaca ggttgaagag cagcgcgaac aggaagaagt gcaagtgcag ccgatggtga
    47161 ctgaggtgcc tgtcgccgcc gctgtcgaac cggttgttag cgcgccagtt gttgaagaag
    47221 tggctgaagt cgtagaagcc cccgttcaga ttgccgaacc gcaaccggaa gtggttgaaa
    47281 cgacgcatcc tgaagtaatt gccgccgcgg taactgaaca gccgcaggtg attaccgagt
    47341 ctgatgttgc cgtagcccag gaagtcgcag aacacgcaga accggtggtt gaaccgcagg
    47401 aagagacggc agatattgaa gaagttgccg aaactgctga ggttgtagtt gctgaacctg
    47461 aagttgttgc tcaacctgcc gcgccagtcg tcgctgaagt cgcagcagaa gttgaaacgg
    47521 taaccgcggt taaaccagag atcaccgttg agcataacca cgctaccgcg ccaatgacgc
    47581 gcgctccggc accggaatat gttccggagg caccgcgtca cagtgactgg cagcgcccta
    47641 cttttgcctt cgaaggtaaa ggtgccgcag gtggtcatac ggcaacacat catgcctctg
    47701 ccgctcctgc gcgtccgcaa cctgttgagt aataattagc tcaagtaatc aagccctggt
    47761 aactgccagg gcttttttat ttcatctttg aatcaacata ttaaaattca tcgaatggca
    47821 tccttgctaa ccaacaatgc aaaataggca aaaaaatgcc cgccccggcg atcccagagc
    47881 aggcagacaa aacataccca gtttcatgat atgttttaaa gcgaaattat ttgttgagct
    47941 ggaagagcga caatccctgc atatcggtaa atgctttata cgatgcctgc aatgccgttt
    48001 gctgcatgat gtaagatgaa atagttgcat tccagtccac atcaaccaga tcgctcatct
    48061 gctgcgtttg ccctaaagcg cgatcgctac ctaatgaatc cagcgactcc agttcgttca
    48121 gctgcgtgcc taattccgcg cgaacagtca gcacattgtt cagcgagttt ttcagtccgc
    48181 ggttggtttt atccagtgcc gcagcggcgg tttctttatc cgcttcgcta tccgcgaccg
    48241 gcgttttcag ggctgcgatg gcactatcca gcatggcaaa aagattggtt tcagaagcgc
    48301 taccgcctgg ttccgctacc gcgttgctgg taatactgtc gaaaattttg tcacccgtgt
    48361 gccctattac catcgaacgc gaagcatcga cctgttgttt aatactttct gttccgccga
    48421 cataatcacc gttagcctca ctaaacggcg cagtctctgt tttataaccg gcaaaaatgt
    48481 agcgcccgtt accgtcagtg gtgttcgcca gattcagcaa ctggtcacga agcccctgaa
    48541 tatccgtagc cagcgaggcc cggtcatcgt cactcaaagt gccattgctg gcgtagacaa
    48601 ttttttcctg agcattctgg atagctgtgg tgacctggct aagtacactc tcttccagtg
    48661 acactttttg agtggcgaaa gtacgcgcca gcgtgtactg gctgttttgc gcctgtgcct
    48721 gggagagaac tacggcttgt gatgctgcaa tgggatcgtc agaagggtta acgactcgct
    48781 tacctgtcga catctgctcg ccgtacttca tccattccgc ctgagaattg gtgatgccac
    48841 gcatgttttg ctggtacatc atctgggtac tgaaacgcat cttatctccc cttagcgaat
    48901 gttaatcagc gcatcaaaaa tcgcgtttgc cgtctgcaga acctgcgcat tcgccaggta
    48961 atactgctga aaacgttgca gatttccgta ctcctcatcg agattgacac cggaaatcga
    49021 ctgctgctga ttggaaagct gcgtcaccac attaccttgc gtggtgctac tggttttcaa
    49081 cgtcgcggtt ttattaccga tatcactcac taacgaggca taagcgtcgt taaaggattt
    49141 cgcaccgccc accgttttac tgttgctttg cagatccagc agggcctgac cgttgcggtt
    49201 gtcgctatca cccgcatctt cttcgctcgc catcgcgatt ttcgcttcgt cggtgattaa
    49261 tacatccatg ttgacgatgg cgtcacttac tggtttcagc gtgaagctgt cgttaacggc
    49321 aggcgttcct gtaaacgtca actccagacc atcaaatgcc actttaccgt tggcatccgg
    49381 cgtcaccgta aaagtggtat tgctggcaag gcgggtgacc tgccactgat tattatcgaa
    49441 cgagattttg taatctgtcg ccagtaccgc ggaggcatca gttaccgtgg caccgatcgc
    49501 aacgttaccg ttgtttttcg tgttttgcag aaccgcgggc ttaccgatag caaagaaatc
    49561 ttcaccctca tcgccgttgg catcaaatcc ggctttgtgt tgggtgttga aagcctcagc
    49621 aaatgccagc gccagttgtc caagcgtatt acgcgtctgg tccagatcct gagaacggaa
    49681 tgtcagaatg ccgcccagcg acccggtatt cagtaatttc tccgggatct caatattgcc
    49741 tgccgtccca tcaacataag cgacagtcgt acgagaaggg tcggcgctgg aaggaactgc
    49801 cgccaattgc cgcgccgtac ttccctgaac cagtgagtaa ccattggcca tcgtgatgtt
    49861 ataagtgccg ccatcctgaa cgctgacttc tacaccaaca atctggttta attcgctcac
    49921 cagttgatcg cgttgatcca gcagattgtt aggtgacgcc cctgccccca cgcctgtcag
    49981 gcgggagatt tgatcgttca ggctggcgat ttgtttagcg taattgttga tctgatcaac
    50041 gctggcacct atcgcgatat tgacctgttt gtcctggtcg cgaagatact gatcggtggt
    50101 tttaaactga ttcaccaacc cttctgattt cccaatcagc gcctggcgcg ctgccgggtc
    50161 ttccgcgtta ctcaccagcg tttgcaggct ggtgaagaaa tcctgcatct gtgttgccag
    50221 cgaagaggta ctggtggaga gcatattgtc gattttcgac atctgctcat agcgggcagt
    50281 caggccgcta ctttgcgtct gcgccgcacg taactggttg gtaataaatg catcatactc
    50341 acgctgcaca ccagaaacgt agacgccatt gccaacccag ccgccagcgc ccaacgtgct
    50401 attggcctgc gccataatag tagtttggcg ggtatatccg gcaacgttat agctggagat
    50461 attattactt gctgtattta acgccgcctg ggccgcgttc agtccgctca tggcgttatt
    50521 gatcaagctg gacatggagg ttccttataa gccttcaatt acagagtatt atcggcagcg
    50581 ccccgccgga cttgagttat tcagaacaga ttatcaatgt tcatactgta ggttttgctc
    50641 accttgtcgc ttatcgattt catctgctga atcatgttgg tgagtttacg ggcatagtga
    50701 ggatcggtgg catagcccgc gtcctgtagg gcctgcgccc cctgctccgc actcgcggcg
    50761 gtcgtcacgg cggcgtagcg cgggttacgt gttaacagcc caacgtaatc cgacaatgct
    50821 tccagatacg agctgtagac ccgaaacttc gcttttactt tcttcgcttc gccattttca
    50881 tattcagtcg tggtgatttc agtgactggc cctttccagt tgccagaggc tttgacacca
    50941 aacaggttat agctcggctc gccgttttcg cggcggattt gccgttgccc ccagccagat
    51001 tccagcgccg cctgagcgag gatcaaatga tgtggcacac cgctttgctg gcttgccagt
    51061 tgggcgggca gcgagagttg cgcgaggaat gctttactgt cacctggcag cgaatcatcg
    51121 tagttacgtg gcacggcctt ttgcaccagc tgcgaaagcg cctgattttg ataacgcacc
    51181 acggtttcga gcgggaattt catcggtgct gctggcgtgg actcctctgg caatggttgt
    51241 tctggcgtca tctgcttaac catcatctct gcaagcccca gacctttgcc cgccgtcatc
    51301 tgttgggcaa tctgctggtc atacatactg gtatacaggc gagtgtgctc gctgctgaac
    51361 aggccatctt ttggtaaagc gtcgcgcatg cttttcaaca tcatctgcac gaacatccct
    51421 tccacctgac gggccaccgg acggatattt gccgccgggt cttcgcccgc tttcgccttt
    51481 agttcgttga gtgattgcgc atcccaggcc gcacttgcca gtagtttgct gtcgctgatc
    51541 atcagatgat ttccagtttt gcccgcagac atcccgcact ttgcattgat tgcagtatgg
    51601 acattaaatc cattggggtg gctcccaagg cgttgagcgc gcgcaccacg ttattgaggc
    51661 tggcgctgga acgtacgctt tgcagcgaac cgccgctctg gcgtaaatcg atctgcgttt
    51721 gtggagtaac cacagtctgt ccaccaccaa acggtgtatc tggctggctg acattggcct
    51781 gacgattgac tgtaactgag agattcccct gcgctaccgc gcagctgtcg agggtcactt
    51841 cgcgattcat caccaccgaa ccggtgcgcg agttaatcac tactttagcg tcctgcgggg
    51901 tgacattaac ctgcatattc tggatatcgg caaggaaacg gacctgggag ctgttgccac
    51961 ttggtacgcg cacctgaata gtccgcgcat ccaacgcggt ggcgctgcca tatccacgca
    52021 ctcggttgat ggtgtcagcg atttgctgcg ccatgctgaa atcttcgtcg ttaagttgca
    52081 aattaagggt attcccgacg ccaaactggc tgggtaattc acgttcaata actgcaccat
    52141 tggtgatccg tccaccgttc agttggttca cctgaacact gctaccgcca gcggaggctc
    52201 cagcgccgcc aaccagaata ttgccctgtg ccagcgcata cacctgactg tcaacgccct
    52261 taagcggtgt catcaacaac gtacctccac gcaagctttt ggcatttccc atggaagaaa
    52321 ccaccacatc gatggtttgc ccctgacgtc caaacggagg aagtgacgct gtcaccatta
    52381 ccgcagcgac gttttttagc tgcatattgg tgcccgtcgg aacggtaatt cccagctgtg
    52441 agagcatgtt attaagcgtt tgtgtggtaa acggcgtctg ggttgtctgg tcaccggtgc
    52501 catccagccc caccaccaga ccatagccaa tcagtgagtt ttgccttacc ccctgaacac
    52561 tggtgagatc gcgaatacgc tcagcctgag ccgccgtcgt aaccagtaga agaattaatg
    52621 cagagagaaa tttaatcacc acagcctcac ttacattggc gacaggttaa ggaagaaacg
    52681 ctgcaaccag cccatatttt gcgcttcgtt aatgtagcca ttgcctacgt attcaatgcg
    52741 cgcatccgcc acctgagtag acggtacggt attgctgccg ctgatggtgc gtggattaac
    52801 cacgccagag aagcgaataa attcggtacc ctgattaatg gcaatctgtt tttcacccac
    52861 cacatgcagg ttgccgttga ccagtacctg gtcaaccgtc accgtcaacg tgccgctaaa
    52921 ggtattgctg gcattggccc cgccctttcc gttgaacgtg ttaccaccgg aggcttcgac
    52981 atcggcacga gcgttaccaa acagcccctg caaatagcgc ggcacagtat caaagccaaa
    53041 attagtttta ccgtcacggc tggcattcgc agaggagctt ttgctggcgc tgacgttctc
    53101 ctgcaacacg atggtcagcg tatcgccaat attgcgtggt cgacgatctt caaacagcgg
    53161 ttgatagcca tagttaatcg gctgagcaga ctggaaaata gaaccgttgg cgacgggcgt
    53221 cggaccggga accggttgtg cactggtcgc cccctgcacc agcggcgtgg agggtatcca
    53281 ggcgcagccg gttagtgaaa gcaccaacaa gctggaaatg gcataagtat gcgcagcgtt
    53341 tttttgcatg gctatcatct tcaaaaatca ataaaccggt gaacctgcca ccggttatgc
    53401 cttagagttg cgtcagtttt tgcagcatct gatcggtggt ggacaccgct ttactgttga
    53461 tttcgtaagc gcgttgcacc tgaatcatat tgaccagttc ttccgccaca ttgacgttag
    53521 acgtttcaac atacccttga tacagcagtc ccgcgccgtt caggcccggc gtgctttcgt
    53581 tcggtgcacc ggaggattgc gtttcggtgt agaggttttc gccaatgctc tccagcccgg
    53641 tgtcattcat aaaggtggtg agattgagct gcccaacctg aaccggagct gcctggcctt
    53701 gttgggttac gctgaccacg ccatcacgac cgatggtgat acttaacgca ttcgccggaa
    53761 tggtgatcgc tggctgcacc tgaaaaccac cagccgtcac cagctgcccg ttctgatcca
    53821 cctggaaaga gccgtcacgg gtataggctg atgagccatc tggcaacatc acctggaaaa
    53881 agccctgccc tttaatcgcg acatctttgc tgttgttggt ctgcgacagg ttgccctggc
    53941 tgtgtaagcg ttcagtggcg accgggcgta cccccgtgcc gatttgtaat ccggagggta
    54001 aggtggtttg ttcggaagac tgtgcccccg gctggcgaat ggtttgataa agcagatctt
    54061 caaacaccgc gcgctgacgc ttaaaaccgt tagtactgac gtttgccagg ttgttggcaa
    54121 tgacgtccat attggtttgc tgggcgtcaa ggcccgtttt ggcgatccat aatgaactga
    54181 tcataaaatg tcctgtagtg gattagtgac aaagtgcata tgtcttcttg ccggatgcgg
    54241 cgtaaacgcc ttatccggcc tacaagtgct cgcaaaatcg ataaattgcg attgccccca
    54301 tagacccgat aaacttgcgc atcgggcaac ttatacttgt catgtatcct ttcaattaac
    54361 tcatcgacag cagttggttg gcacggcctg cgttatcatc gacgctgctg atcaccttca
    54421 tctgcatttc aaaacgccgc gcgctggcaa tcatgtcgct cattgccgca acggtattga
    54481 cattactgcc ttccagaaca cccgacatca cacgcaaggt tggatctgcc tgcagtacaa
    54541 gcccacgcgt ggcctgggtt tctgcgctta aacgaaaaat gccgtcgtca ccgcgctgca
    54601 cttcgctgcc cgtggctttc accagtttaa gacgccctac tggcgcaacc gtatttgccg
    54661 gatcgcccgg attcagcgcc gagattgtgc catcggcagc aatagtgatt tccgcccctt
    54721 ccggcacagc aattggcccc gcctcgccta tcaccggatg cccctgaatt gtcagttgcc
    54781 cggtgggatc aacctgaatg ctgccattac gcgtataccc ttcgctgccg tcagcggtct
    54841 gcacggccag ccagccatcc tgctgcaatg caacgtccag cgggcgcgag gtgtaatcca
    54901 tcttgccggg cgtcatgtct gcacccggtg ttgacgccgt taccagcgtg cgcgtgggca
    54961 gtgaaagccc ttccaccggc actgcgcgca aggcgtttaa ctgcgcgcga aaaccgggcg
    55021 tagaggcatt ggccagatta ctggcggtta ccgcctgttg attcagcgtc tggctggctg
    55081 ctcccatcgc ggtataaatt gcgtgatcca ttgagctatc ccgtcagcga ttagcgtaag
    55141 ttaaccagcg tgttgaggat ctggtcctgg gttttgatgg tctgggcgtt agactgatag
    55201 ttacgctggg caacgatcat attgaccagt tctttactga gatcgacgtt ggacgcttcc
    55261 agcgcaccgt tggtcagggt gccaaagttt cccgtcccgg ctgtccccaa cagcgccacg
    55321 ccagaagatt gcgtcgcaga ccagacgttg tcgccttcgg atgccagacc ttcgttgttg
    55381 gcaaagttcg ccagtacaat ctgccccagc agttgggttt gttcattgga atagttgccg
    55441 acaaccgtac cgtcatcatt gatttgataa ctcaccagat cgcccggttt gtagccattc
    55501 tgggtggttg ccacaatgtt gttagcgccg gtattttgct gcatggagtt gaggaagctc
    55561 aggctaaacg tggcgggttc tgcaccgtta attgcgccgg ttgcgatatt attcgccatc
    55621 gcaccatcca ctaatgtgcc attagcatta aatttcagtg ttgtcgctgt cttcgcaatg
    55681 ctgtttggat cactgctatc ctgggtgtag acctgccagt tattatcccc ggtcttcaca
    55741 aagtacacgc tcatgtcatg agcattaccc tgactgtcga aaacagtcac cgaacctttt
    55801 ttgttatagc tatccgcatt gctggcatca aatgcgttaa cagagggaag cggatcgctg
    55861 gaattcaggt tgatctgcat cgacgccgtg gtggtagttt tcgctgccat cagggtattc
    55921 gggatcgaaa tattggtcgg attcgcccct tgctgaatag tcggcggcgt accggttgcc
    55981 gggtaacccg tcagctgtaa accttgcata ttcaccaggt tacgattttc atccagctta
    56041 aattgtccgt tacggctgta gaacaccgaa ccgttgctgt ctaccagacg gaaaaaaccg
    56101 ttctggctga tagcaacgtc cagacctcgc ccggtgttgg tggtcgtgcc atcggtaaag
    56161 tcctgagtga taccggcaac ttttaccccc agtcccactt tcgaaccggc aaacatatcg
    56221 gcaaaagagg ccgtgcctga tttaaagccg tatgtggcgg agttggcgat attgttgcca
    56281 ataacatcga ggttggtggc ggcagcgttt aatccgctaa ccgcttgaga aaaggccatg
    56341 actgactcct gaaatgtgaa ggcttaaatt atctgccgta cttcgtcgag ggtggtggtg
    56401 ccgtaagtgc cgagatccag cgtattaccg ctgttgccgc ggatcacacc ctgcaccaga
    56461 gcaaactgca gcggctgggc aaccagttgt gtaccaccgt tactggcgct aatcgctaca
    56521 ttgtaagaac cgttcggcgc agtgctgcca tcagtcaacg taccgtccca ggtgaaactg
    56581 tgaactccgg cggtcagttc accaatatca atggtgcgca caaccgcgcc atttttatcg
    56641 gtgatggtgg ccgtaacttt gtctgccgcc tgttgcagct caacaccaaa cggcgtggtc
    56701 gtggtcacag ccccttcttc actgccggtt cccgcaagaa cagtggtgcc ggggatcatc
    56761 acgccgtgac cgatcaggtt actggcctgt aacgactggc tgttatcaat ctgtccggaa
    56821 atagatccga gcgtggtatt gagtttttca atcccgctga ccgtgctgat ttgtgccaat
    56881 tgcgacgtca gctcgttgtt ttccattgga ttggtcgggt cctggttttt cagctgcgcc
    56941 accaacaaag tcagaaaact gctttgtaaa tctgcggcgt tgctgcccgt gagcgaacta
    57001 ctgctggtgg tactgacgcc ggtatttgtc ggatcggtgg tggttaccgc aatggacata
    57061 gctttctcct ttattgaccg agcgtaaggg ttttcagcat catgcttttc accgtgttga
    57121 gcacttcaac attggcctga tagctgcgtg acgccgacat ggtgttaacc atctctccga
    57181 caacatcaac gttcggcatt tttacgtagc cctttgcatc cgccagcgga ttacccggtt
    57241 cataaaccag tttgtccggg gcctgacttt ctataacatc ggcaaccttt acgccgcctg
    57301 tcgcagcacc tggtgcagcg ttaacctgaa ataccacctg ttttgcccga tatggctgtc
    57361 catcgggacc ggtcacgcta tcagcattcg ccagattact ggccgccacg ttcaggcgct
    57421 gggactgggc agttaacgcc gacccggcga tatcgaaaat attcagcagt gccatccgtt
    57481 aatttccgct ctgtaaaacg ttcatcatgc ctttgatttg cccgctcaac gcactaaggc
    57541 tcatctggta ttgcaggctg ttatcggcaa actgggtgcg ttcgcgatcc atatcgacgg
    57601 tattaccgtc aagcgaaggc tggtccggaa tacggtattg cagttctgcg gtaggagtcg
    57661 tcagcgcctg cgccggaatg tgttgcgttg aggtcatcgt cagtgcaacc acactggttg
    57721 catcccgtcc acgttgcatg acttttttaa gttcactggc aaaatcgata tcgcgcgcct
    57781 gataaccagg ggtatcggca ttggcgatgt ttgctgccag cacttcctga cgctgggcgc
    57841 gcagattgag cgcctcttgt tgaaaacgta aggcggcgtc gagcttatcg agcatatctc
    57901 ctccgcaggt atcaaaattc tgccatcagc ttaaatgcct ttacgcgcgc gttatcggcg
    57961 gaataaacgc aaaatgggtc gctatttatg ccgttgatgg tcattgcgga caggtacaat
    58021 tcacgttgta gaaatggctg ggggcgaaaa tgctggcaat aaaacgtagc gtggcgatca
    58081 tcgcgatact gttcagtccg ttaagtgcgg cgagcaatct cacatcgcaa ttgcaaacct
    58141 tttttagctc ccagctcgca gggataagtg atgaggttcg tgtttctatt cgtacagcgc
    58201 ccaatctact accgccgtgc gagcagccat tgctttcgat gagcaataat tcccgcctgt
    58261 ggggcaatgt gaatgtgttg gcacgctgcg gtaacgacaa acgatattta caggttaatg
    58321 tgcaggccac aggaaattat gtggttgccg cgatgcccat tgtgcgggga ggaaagctgg
    58381 aagctggcaa tgtcaaacta aaacgcggac ggctggatac cctgccaccg cgtacggtgc
    58441 tggatatcaa tcaacttgtt gatgccgtta gcctgcgcga tctatcaccc gatcaaccta
    58501 tccagttaac ccagtttcgc caggcatggc gggtaaaagc gggacaacgc gtcaatgtga
    58561 tcgccagcgg tgatgggttt agcgccaacg ctgaaggtca ggcgctgaac aatgcatccg
    58621 tcgcacagaa tgcgcgggtg cgcatggtat ctggacaggt agtcagcggc gttgttgatg
    58681 cagatgggaa tattcttata aacctgtaag ctgttaaaga ttacccgtcc cttgccgata
    58741 aataagcaac acatgataaa agcgccctca atgaggaata aaccatgagt attgatcgca
    58801 cttcgcctct gaagcccgta agcaccgttc aaccgcgcga aaccactgac gcgccggtaa
    58861 cgaacacccg ggcggcaaaa acaacagcat ccaccagcac cagtgtgacg ttaagcgacg
    58921 cgcaagcaaa actgatgcaa cccggcagca gtgatatcaa tcttgaacgt gtcgaagcgt
    58981 taaaactggc gattcgtaac ggtgaactaa aaatggacac cggcaaaatt gccgatgcgc
    59041 tgatcaacga agcgcagcaa gacttgcaga gtaattaatc gtatgacgcg tcttgcagag
    59101 atcctcgacc agatgtccgc tgtgcttaac gatctcaaaa cggtaatgga tcaagagcag
    59161 caacatctct ctatggggca gatcaacggc agccagttgc aatggattac agaacaaaaa
    59221 agctcactgc tggcaacgtt ggattacctc gaacagttac gcaggaaaga acccaataca
    59281 gcaaatagcg ttgatattag tcaacgctgg caggaaattg ctgtgaaaac gcagcaacta
    59341 cgccaaatga atcaacataa cggctggtta ctggaaggac agattgagcg caatcaacag
    59401 gcgctggaaa tgttgaaacc gcatcaggaa ccgacgctat atggggcgaa cggtcagacc
    59461 tcaacaaccc atcgcggcag taaaaagatt tcgatctgaa gattatctcc ggcctgcact
    59521 gcaggccgga atgcattgtt acaccgtccg gcgggcaaat tctttaactt tgaagcccag
    59581 taccgccagt gcagcgaagt acgcggcaat ccccgccagc acgaccgcca ttaaacgcag
    59641 caagcgccag ggcatggtac ccaatgacca ctccggcatg atatgtaaca tacctaaaag
    59701 cacgccagac atcaccagta ccgccaccac cagacgcaac agaaacgcca tccagccagg
    59761 ttgcggggta aagattttct gcttacgcaa ctgccagtaa agcagcgaag cattcagaca
    59821 cgccgccaga ccaatagaaa gtgacagccc ggcatgtttc aacggaccga taaacgccag
    59881 gttcatcaat tgcgttaaaa tcagcgtaac gatggcaatt ttcactggcg ttttaatgtc
    59941 ctggcgggaa taaaagccag gagccaacac tttcactaca atcaggccaa tcaaacccac
    60001 cgagtaggca attaacgccc gctgggtcat cagcgcatca aacgcggtaa atttaccgta
    60061 ctggaacagc gaaacggtca acggacctga aagaatgccc aacgcaaccg cactcggcag
    60121 cgccaacagg aaacaaagac gcagccccca gtccatcaaa cggttgtatt catcatgatt
    60181 gccactggca aaacttttcg acagcgacgg cagcaaaatg gtgccaagcg ccacgcccag
    60241 cacaccggac ggaaactcca ttaagcggtc ggcgtaatac atccacgaca ccgaaccgga
    60301 agcaagaaac gaggcaaaaa tggtgttgat gattaaggag atctggctca cagagacgcc
    60361 aaggattgcc ggtcccatct gtttcaccac acgcattgct ccggcatcgt ggaagttaat
    60421 gcgtggcaaa accagcatgc cgatcttctt caggtgcggt agctgataca ccagctgcag
    60481 gacgccgccg accgtaacag cccacgccag cgccagcacc ggtgggttaa agtacggtgc
    60541 ggcaaacagc gcgaaaccaa tcatgctgat gttaagcagt gttggagcaa acgccggaat
    60601 cgagaagcgg ttccacgtat tcagaatcgc tcccaccagc gacgccaggg agatcagcaa
    60661 gatataggga aaggtaatct ttagtagctg gctggtcagg gcaaatttgt cagctgtgtc
    60721 agcgaagcct ggcgcggtca ccatgatcac ccacggtgcg gcgagcatgc cagcgaccgt
    60781 cacaaccgcc agcgcgagtg tcagcaggcc agaaacataa gagacaaaga cccgcgtggc
    60841 gtcttcaccc tgcttacttt tatattccgc cagaatcggt acaaatgcct gggaaaatgc
    60901 cccttcggca aagatacggc gtaacaagtt aggaagttta aaagcgacga aaaaggcgtc
    60961 ggttgccatc cctgcgccaa agattctggc gacaattgcg tcacgtgcga agccaagcac
    61021 acgcgaaaac atggtcatcg agctgacggc ggccagcgat tttaataaat tcatcggtgt
    61081 tctaatccag acccacgggc aaaacgcctg caaggcaggc gctgaaagag gcgaatagtc
    61141 tacctggatt atggtgaatt gctaccgcca gatgttacag ggtttattca ctcatcgcat
    61201 cgcgccagat cttgtcaacg atacgttgcg ccagcacggc ctgttcgccg gcggtttgcg
    61261 gaactgtctg attttgcaca cattcaatga agtgccgcgc acagccgaca aatccacgtt
    61321 gctcaagcgt gctctgccaa ccaggaatcg gtttattcac tacaccctgc ccacgctcct
    61381 cacgccattc gcgcatttcc gtaatgtcga tgagcgcacc gtcagtcacg gcctgcacgg
    61441 tttcacgctg acttccggca cggcgatgca tacaggtggt gatctgcaaa ggaccggccg
    61501 aaaaatggtg ctcggcaaac agcatttcgc cagcgtcgtt agtcagtagc gtaccgccat
    61561 ccagagaggc tttgccgccc gacaaccaca gcgcggtatc caccacatgc agataatcat
    61621 ccagcaacgt gaaataaaga tcgtgtggcc cggcgctatt gctacgatgt ttatccattc
    61681 ttagcgaggc tgcggtggcg agttgcgttt ttaactcacc gtagagtggt gcgaaacgac
    61741 ggttaaaacc gaccatcaac gtcagttttt tccgcgccgc cagttccacc agccgttcag
    61801 catcgcgcag attttctgcc agcggtttat cgacacagac atgtaccccc gcattgagta
    61861 acgtactgac cacgtcaaag tgactggcgg tgctggaatg tacaaaaacc gcatcgcaac
    61921 tggcggcaag gctggataac gaatcggcat aaggaatgcg ccagctttca caaattggca
    61981 gggctttcgc gcgcgtaggc gaccaggctc cttgtaacgt ccagtcagac gctgccgcca
    62041 gcaccggtaa ccacgctttt tgcgcaatgc cgcctaatcc cactacgccg atacgtaatt
    62101 ttttcacttt aatctcccag gtgggccagc aacgaatcaa gacgctgttt cagttctgcc
    62161 acttcgattt ccagggcttc gacgcgggcc tgtaaatcac cgtcaaccgc gttcgacata
    62221 tccgttaccg ccggctgatc ctctacctca ccactgaaaa gatgcatgta gcggctttcg
    62281 cgtttacccg gttcgcgggc cagacgcacc acaaaaggac catcttcgcg atttgccagt
    62341 tgttccagcg tcgactccac ttccgccata tcgctgaatt catacattcg cgcggcgcgg
    62401 ctgcgcagtt cacccggcgt ctgggcacca cgcaataaca acgtggtgat taacgccact
    62461 tctgcggcgc tcagcttcag atcgccaaat tctgaattac aaaaacgttg ttcatatttg
    62521 gtgactcgat taccaaagcc actcaccgtg cgcagataat ggcgttttac cagattatcc
    62581 agttgctcct gcacctcgga ttcactcaga ttcatgaccg gttcacggtt cgttttctga
    62641 ttacaggccg ttactacacc attgactgag agcgggtatt gctccggcgt ggtcacctgc
    62701 ttttccagca agcagccaat cacgcgggct tccagagcag taagttgata tttcatgaga
    62761 ttctccttag cggccgggcg tccagtctgg ggtagttaat gccgtcagta cgtgatcgcg
    62821 ccattgtcca tcaatcaaca gatagtcttt cgcatagcct tctttttcaa aacccagtcg
    62881 tgccagtaaa tcaccgctgc gtttattgtg cggcatataa ttagccataa tgcgatgaat
    62941 atgttgggtg cgctgcatat aacgaatggc tgcggtcaga gcttcaaaca tgagtccttt
    63001 gccctgccat ttttgcccaa tcgaataacc gagatagcag gcatgaaaag agccgcgaac
    63061 aacattggaa aaattggcaa cgccaataat ctctttttcg tccgggtcga ataaaccaaa
    63121 gtagaaagct gaaccttgtt tatgaaattc gttaatcatc cccagcctgg cctgccagcc
    63181 tgatggatag cagtggcttt cgtcgcgcac tggctcccag ggcttgagga aatggcgatt
    63241 ctctgcgtaa taatccgcca gacgccaggc atcacgatca tgcaccagac gcacgaccag
    63301 tcggtctgtg gttaagcgca cttttggcac gttactgcga tagccaaaca ttctatacct
    63361 actccttccc gtaacgtctt taatacgctg taagtattac tatacctgcg caaccagacc
    63421 ctgtgaaaag tcccaaatgc cattatctgc attattcaca tatttgataa aaactctcta
    63481 tcaaagcgac gttttgataa aaaaatattg tccccgaagt gatgggaaaa gtcttagcgg
    63541 caatgcagaa tagaaagtgc tcatctttta tttcccggga ggggaaatgt cccgcgtgtc
    63601 gcaggcgagg agcctgggta aatatttcct gctcatcgat aatatgctgg tcgtgctggg
    63661 gttctttgtt gtcttcccgc tgatctctat ccgcttcgtt gatcaaatgg gctgggccgc
    63721 cgtcatggtc ggtattgctc tcggtctacg ccaatttatt cagcaaggtc tgggtatttt
    63781 cggcggcgca attgccgacc gctttggtgc caaaccgatg attgttaccg gtatgctgat
    63841 gcgcgccgcc ggattcgcca caatgggtat cgcccacgaa ccgtggctat tgtggttttc
    63901 atgcctgctc tcgggactcg gcggcacgtt gtttgatccg ccgcgttcgg cgctggtggt
    63961 gaaactaatc cgtccacaac agcgtggtcg ttttttctcg ctgttgatga tgcaggacag
    64021 tgccggtgcg gtcattggcg cattgttggg gagctggctg ttgcaatacg actttcgcct
    64081 ggtctgcgcc acaggggcag ttctatttgt gctgtgtgcg gcgttcaatg cgtggttgtt
    64141 accagcatgg aaactctcca ccgtacgcac gcccgttcgc gaaggcatga cccgcgtgat
    64201 gcgtgacaag cgttttgtca cctatgttct gacgctggcg ggttactaca tgctggctgt
    64261 acaagtgatg ctgatgctgc caattatggt caacgacgtg gctggcgcgc cctctgccgt
    64321 taaatggatg tatgccattg aagcgtgtct gtcgttaacg ttgctctacc ctatcgcccg
    64381 ctggagtgaa aagcattttc gtctggaaca ccggttgatg gctgggctgt tgataatgtc
    64441 attaagcatg atgccggtgg gcatggtcag cggcctgcaa caacttttta ccctgatttg
    64501 tctgttttat atcgggtcga tcattgccga gcctgcgcgt gaaaccttaa gtgcttcgct
    64561 ggcggacgca agagctcgcg gcagctatat ggggtttagc cgtctgggtc tggcgattgg
    64621 tggcgctatt ggttatatcg gtggcggctg gctgtttgac ctgggcaaat cggcgcacca
    64681 accagagctt ccgtggatga tgctgggcat tattggcatc ttcactttcc ttgcgctggg
    64741 ctggcagttt agccagaaac gcgccgcgcg tcgtttgctg gaacgcgacg cctgatttga
    64801 tactcccccc tgactcctcc atactgaaag tgaactgaca gccaaatgga ggagtcacgt
    64861 gaagctatac atttacgatc actgccctta ctgcctcaaa gcccgcatga ttttcggcct
    64921 gaaaaatatc cccgtcgaat tacatgttct gctcaacgac gacgcagaaa cacccacccg
    64981 gatggtcggt caaaaacagg ttcccattct gcaaaaagat gacagccgct atatgccaga
    65041 aagcatggat atcgttcact atgtcgataa actcgacggc aaaccgttac tgaccggcaa
    65101 acgttcccct gccattgaag agtggctgcg caaggtcaat ggctacgcca acaaactgct
    65161 gttgccgcgt tttgccaaat cggcatttga tgagttttct actcccgccg cgcgcaaata
    65221 tttcgtcgac aagaaagagg ccagcgcggg taattttgcc gacctgctgg cccactctga
    65281 cggtctgatt aagaatatca gcgatgattt acgtgcgctg gacaaactga tcgtcaaacc
    65341 gaacgccgtg aatggcgaac tttcggaaga tgatattcag ctatttccac tactgcgtaa
    65401 tctgacgctg gtagccggaa tcaactggcc aagccgcgtt gctgattacc gcgataatat
    65461 ggcgaaacag acacaaatca atttgttatc atcaatggcg atttaatcct gacccgccgc
    65521 ggcagcctgt attcgcgggt tgccgccgtt ttcagttttt tctgctggcg agtttatcgc
    65581 tggtgacaca tgctgcgttg tgtcaggata ttgacgcaga tttcatgtcg aggactacca
    65641 tgaacaagtt tttatttgcc gctgcattga tcgtcagtgg cctgctcgtt ggctgtaatc
    65701 aactcaccca atacaccatc accgaacaag aaattaacca gtcgcttgcg aaacataata
    65761 atttctcaaa agatatcggt ttacccggcg tggctgacgc ccatattgtt ctgacaaacc
    65821 tgaccagcca aattggtcgc gaagagccga ataaggttac cctaaccgga gacgccaatc
    65881 tggacatgaa ctccctgttc ggtagtcaga aagcgaccat gaaactgaag ctgaaagcgc
    65941 tgcccgtgtt tgataaagaa aaaggtgcga tcttcctgaa agagatggaa gtggtcgatg
    66001 cgacggtaca accggaaaaa atgcaaacgg tgatgcaaac gttgcttccc tatttgaacc
    66061 aggcattacg caattacttt aaccagcaac ctgcttacgt cctgcgcgaa gatggcagcc
    66121 agggcgaagc aatggcgaaa aaactggcga aaggcattga agtgaagcca ggcgaaattg
    66181 tcattccatt tactgattaa tcacgagggc gcattcgcgc cctttatttt tcgtgcaaag
    66241 gaaaacgttt ccgcttatcc tttgtgtccg gcaaaaacat cccttcagcc ggagcataga
    66301 gattaatgac tgcaccatcc caggtattaa agatccgccg cccagacgac tggcaccttc
    66361 acctccgcga tggcgacatg ttaaaaactg tcgtgccgta taccagcgaa atttatggac
    66421 gggctatcgt aatgcccaat ctggctccgc ccgtgaccac cgttgaggct gccgtggcgt
    66481 atcgccaacg tattcttgac gccgtacctg ccgggcacga tttcacccca ttgatgacct
    66541 gttatttaac agattcgctg gatcctaatg agctggagcg cggatttaac gaaggcgtgt
    66601 tcaccgctgc aaaactttat ccggcaaacg caaccactaa ctccagccac ggcgtgacgt
    66661 cgattgacgc catcatgccg gtacttgagc gcatggaaaa aatcggtatg ccgctactgg
    66721 tgcatggtga agtgacacat gcagatatcg acatttttga tcgtgaagcg cgctttatag
    66781 aaagcgtgat ggaaccgctg cgccagcgcc tgactgcact gaaagtcgtt tttgaacaca
    66841 tcaccaccaa agacgcagcc gactatgtcc gtgacggaaa tgaacggctg gctgctacca
    66901 tcactccgca acatctgatg tttaaccgca accatatgct ggttggtggc gtgcgtccgc
    66961 acctgtattg tctacccatc ctcaaacgca atattcacca acaggcactg cgtgaactgg
    67021 tcgccagcgg ttttaatcga gtattcctcg gtacggattc tgcgccacat gcacgtcatc
    67081 gcaaagagag cacctgcggc tgcgcgggct gcttcaacgc cccaaccgcg ctgggcagtt
    67141 acgctaccgt ctttgaagag atgaatgctc tgcagcactt tgaagcattc tgttctgtaa
    67201 atggcccgca gttctatggg ttgccggtca acgacacatt catcgaactg gtacgtgaag
    67261 agcatcaggt tgctgaaagc atcgcactga cagatgacac gctggtgcca ttcctcgccg
    67321 gggaaacggt acgctggtcc gttaaacaat aaaaatcgta gcttcctgtt gtcattaggt
    67381 tattttacct gtataaataa ccagtatatt caacaggggg ctattatgcg aattgaagtc
    67441 accatagcga aaacttctcc attgccagct ggggctattg acgccctggc tggcgaactt
    67501 tcccgccgta ttcagtatgc gtttcctgat aatgaaggcc acgtatcggt acgttacgcc
    67561 gcagcgaata atttatcggt tattggcgcg acaaaagaag ataaacagcg cattagcgaa
    67621 attctccagg aaacgtggga aagcgccgat gactggtttg tcagcgaata atatgcagtg
    67681 atttttgttt ttgccgggtc gccccggctt ttttatttta cttacgaatt ttgagtacaa
    67741 aatttctaca ct
//
LOCUS       DAZFKF010000017        67399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFKF010000017 DAZFKF010000000
VERSION     DAZFKF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.017"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(377..1222)
                     /gene="sseA"
                     /locus_tag="ACHKA1_001353"
     CDS             complement(377..1222)
                     /gene="sseA"
                     /locus_tag="ACHKA1_001353"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO5679031.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            1429..6390
                     /gene="yfhM"
                     /locus_tag="ACHKA1_001354"
                     /pseudo
     CDS             1429..6390
                     /gene="yfhM"
                     /locus_tag="ACHKA1_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
     gene            6391..8703
                     /gene="pbpC"
                     /locus_tag="ACHKA1_001355"
     CDS             6391..8703
                     /gene="pbpC"
                     /locus_tag="ACHKA1_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311412.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO5679032.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEVA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARQGKAGKLRLQPDDPLLERPLMSSEAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQAQQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIASVHFSFQ"
     gene            8867..11248
                     /locus_tag="ACHKA1_001356"
     CDS             8867..11248
                     /locus_tag="ACHKA1_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HFO5679033.1"
                     /translation="MDTPDEKERITFPQVSRRSFLQATSALITLPFISSTAKAQSPDA
                     SPEVTAPVADKVVPTCSTFDCGGKCDIRAHMRDGVVTQITTLPDNELDPQMPIMRACV
                     RGRGYRKFVYHPDRLKYPMKRVGKRGEGKFERISWDEATTLIADNLKRITQQYGPASR
                     YVHVGTAVSGGTFSGDAMARRLLNLTGGYLEYYHSVSLGNTAAATPYTYGVAASGNSM
                     DTLLDTKLVILWGHNPTETIFGHTNYFFQKMKQNGTRFIVVDPRYSDTVSSLADQWIP
                     LLPTTDNALMDAMMYVIISENLHDKTFIDTYTLGFDENSMPEGVPANESLVAYLFGAK
                     DGIHKTPEWAEKITHVPAQSIRQLARDYATTKPAALIQGWGPQRHTCGERTARGSTLL
                     ASITGNVGIKGGWAAGYGGSSNRKFCVGPDMPENPVQAKISIMNWMQAADDASKVTPQ
                     DGLKGVDKLDSNIRLLFSLAGNYLANQNPDVHQAAKLLEDESKIEFIVLSDLFMTPSA
                     KYADVLLPETSFMERWNIGETWGTASYLILSEKLIEPDFERRTDYDWLRDVAKKLGVE
                     AEFSQGRDEKQWIEHIWEQTRLAMPDENLPDFATLQKTRRHLFKSAPHIAFEANIRDP
                     QNNPFPTPSGKIEIFSKRLFDMQDPEIPALSHYVPAFEGPEDKLTAKYPLQLITWKGK
                     NRANSTQYANPWLQEVQTQKLWLNPQDAKQRGIVEGDSVKIYNDRGVSIIPVEITPRI
                     IPGVVAMQAGAWWQPDAQGIDRGGCANVLSSTRITALAKGNSHQTMLVEVEKA"
     gene            11245..11874
                     /locus_tag="ACHKA1_001357"
     CDS             11245..11874
                     /locus_tag="ACHKA1_001357"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HFO5679034.1"
                     /translation="MSQFKEFASVSDKQLGFFIDSSRCSGCKACQVACKDKNNLEVGR
                     RFRRVYEVNGGGFIATGEGGVSHNVFAYTLSVSCNHCADPICTKNCPTMAMHKRPGDG
                     IVRVNTDKCVGCGYCAWSCPYGAPQMNEQTGQMSKCDFCIDLQAKGEQPICVATCPLG
                     AIKFGPIDELREKYGDVSDVKGLPDSSITQPNLVIKPHQGAEKEASHHA"
     gene            11867..12688
                     /locus_tag="ACHKA1_001358"
     CDS             11867..12688
                     /locus_tag="ACHKA1_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFO5679035.1"
                     /translation="MHELPLMAFTLLLQASVGMTLWLAFLHRHILITSSAPPVMRPPL
                     LLAFLAGAVGLLISTLHLGYPLNAMHALSHFSSSWLSREIIFGALYLALLGLSTLLVM
                     LRKGGWQLLLMLAAVVGIVDVFCMAQVYIHTSIITWQHYNTLIMFLGTVGILGSALVA
                     VFRISGILPQIDALRNGCVLVIALLVLLRLLVQPLWIGDLTANAMQIATLPHAPLAML
                     GQLKPILTLSWGISVIGMMFFAVGGCKKNIPAALFGSVMLVGSEVMLRFVFFSIG"
     gene            12688..13542
                     /locus_tag="ACHKA1_001359"
     CDS             12688..13542
                     /locus_tag="ACHKA1_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO5679036.1"
                     /translation="MLSHNITIGAECARQRFRFATCHACVEACPAQALTLTEEGIAVD
                     AEQCIECAVCQFICPQEAIRGVNSPLRYFRNHTLVAPFTTIAPTLEELLLWHKEQGIT
                     AIAIDLSQAKAWERALLLLNERLRQYGEEGWSVVSPHTSEVNVSRRRLFHVQRADVQM
                     TQIQPGLRRLHYLWPQISDARPMMDPQKCQLCGACWRACEQQVFSLNEGHLQINDALC
                     NGCQNCIAVCFHQAMTVELTILPAKIVNLHANRKVCKTCQKSFLTFQQNAQNCLYCQR
                     HRYGMRAP"
     gene            13682..14113
                     /gene="ndk"
                     /locus_tag="ACHKA1_001360"
     CDS             13682..14113
                     /gene="ndk"
                     /locus_tag="ACHKA1_001360"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO5679037.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            14263..15417
                     /gene="rlmN"
                     /locus_tag="ACHKA1_001361"
     CDS             14263..15417
                     /gene="rlmN"
                     /locus_tag="ACHKA1_001361"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO5679038.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            15702..16715
                     /gene="rodZ"
                     /locus_tag="ACHKA1_001362"
     CDS             15702..16715
                     /gene="rodZ"
                     /locus_tag="ACHKA1_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO5679039.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            16742..17860
                     /gene="ispG"
                     /locus_tag="ACHKA1_001363"
                     /gene_synonym="gcpE"
     CDS             16742..17860
                     /gene="ispG"
                     /locus_tag="ACHKA1_001363"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO5679040.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            17971..19245
                     /gene="hisS"
                     /locus_tag="ACHKA1_001364"
     CDS             17971..19245
                     /gene="hisS"
                     /locus_tag="ACHKA1_001364"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO5679041.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            19263..19883
                     /gene="yfgM"
                     /locus_tag="ACHKA1_001365"
     CDS             19263..19883
                     /gene="yfgM"
                     /locus_tag="ACHKA1_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO5679042.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            19894..21072
                     /gene="bamB"
                     /locus_tag="ACHKA1_001366"
     CDS             19894..21072
                     /gene="bamB"
                     /locus_tag="ACHKA1_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO5679043.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            21190..22662
                     /gene="der"
                     /locus_tag="ACHKA1_001367"
     CDS             21190..22662
                     /gene="der"
                     /locus_tag="ACHKA1_001367"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO5679044.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            22731..22946
                     /gene="yfgJ"
                     /locus_tag="ACHKA1_001368"
     CDS             22731..22946
                     /gene="yfgJ"
                     /locus_tag="ACHKA1_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO5679045.1"
                     /translation="MELHCPQCQHVLDQDNGHAHCPSCGEVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(22943..24313)
                     /gene="xseA"
                     /locus_tag="ACHKA1_001369"
     CDS             complement(22943..24313)
                     /gene="xseA"
                     /locus_tag="ACHKA1_001369"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO5679046.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            24475..25941
                     /gene="guaB"
                     /locus_tag="ACHKA1_001370"
     CDS             24475..25941
                     /gene="guaB"
                     /locus_tag="ACHKA1_001370"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO5679047.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            26010..27587
                     /gene="guaA"
                     /locus_tag="ACHKA1_001371"
     CDS             26010..27587
                     /gene="guaA"
                     /locus_tag="ACHKA1_001371"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO5679048.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFITVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(27680..28219)
                     /gene="yfgI"
                     /locus_tag="ACHKA1_001372"
     CDS             complement(27680..28219)
                     /gene="yfgI"
                     /locus_tag="ACHKA1_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO5679049.1"
                     /translation="MKKVFLCAILVSLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(28235..28753)
                     /gene="yfgH"
                     /locus_tag="ACHKA1_001373"
     CDS             complement(28235..28753)
                     /gene="yfgH"
                     /locus_tag="ACHKA1_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO5679050.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(29053..29244)
                     /gene="yfgG"
                     /locus_tag="ACHKA1_001374"
     CDS             complement(29053..29244)
                     /gene="yfgG"
                     /locus_tag="ACHKA1_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO5679051.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            29262..29414
                     /locus_tag="ACHKA1_001375"
     CDS             29262..29414
                     /locus_tag="ACHKA1_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679052.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            29596..31839
                     /gene="pdeF"
                     /locus_tag="ACHKA1_001376"
     CDS             29596..31839
                     /gene="pdeF"
                     /locus_tag="ACHKA1_001376"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO5679053.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRTVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(31878..33419)
                     /gene="ppx"
                     /locus_tag="ACHKA1_001377"
     CDS             complement(31878..33419)
                     /gene="ppx"
                     /locus_tag="ACHKA1_001377"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO5679054.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(33424..35490)
                     /gene="ppk1"
                     /locus_tag="ACHKA1_001378"
     CDS             complement(33424..35490)
                     /gene="ppk1"
                     /locus_tag="ACHKA1_001378"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO5679055.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(35661..36299)
                     /gene="purN"
                     /locus_tag="ACHKA1_001379"
     CDS             complement(35661..36299)
                     /gene="purN"
                     /locus_tag="ACHKA1_001379"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO5679056.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(36299..37336)
                     /gene="purM"
                     /locus_tag="ACHKA1_001380"
     CDS             complement(36299..37336)
                     /gene="purM"
                     /locus_tag="ACHKA1_001380"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO5679057.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            37661..38287
                     /gene="upp"
                     /locus_tag="ACHKA1_001381"
     CDS             37661..38287
                     /gene="upp"
                     /locus_tag="ACHKA1_001381"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO5679058.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            38373..39662
                     /gene="uraA"
                     /locus_tag="ACHKA1_001382"
     CDS             38373..39662
                     /gene="uraA"
                     /locus_tag="ACHKA1_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO5679059.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            39757..40458
                     /gene="hda"
                     /locus_tag="ACHKA1_001383"
     CDS             39757..40458
                     /gene="hda"
                     /locus_tag="ACHKA1_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO5679060.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(40596..40955)
                     /gene="arsC"
                     /locus_tag="ACHKA1_001384"
     CDS             complement(40596..40955)
                     /gene="arsC"
                     /locus_tag="ACHKA1_001384"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO5679061.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(40976..42439)
                     /gene="bepA"
                     /locus_tag="ACHKA1_001385"
     CDS             complement(40976..42439)
                     /gene="bepA"
                     /locus_tag="ACHKA1_001385"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO5679062.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            42652..43713
                     /gene="yfgO"
                     /locus_tag="ACHKA1_001386"
     CDS             42652..43713
                     /gene="yfgO"
                     /locus_tag="ACHKA1_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO5679063.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVIGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(43751..44599)
                     /gene="focB"
                     /locus_tag="ACHKA1_001387"
     CDS             complement(43751..44599)
                     /gene="focB"
                     /locus_tag="ACHKA1_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO5679064.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(44621..46633)
                     /gene="hyfR"
                     /locus_tag="ACHKA1_001388"
     CDS             complement(44621..46633)
                     /gene="hyfR"
                     /locus_tag="ACHKA1_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO5679065.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATVEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLKKDCDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLIQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(46663..47076)
                     /gene="hyfJ"
                     /locus_tag="ACHKA1_001389"
     CDS             complement(46663..47076)
                     /gene="hyfJ"
                     /locus_tag="ACHKA1_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO5679066.1"
                     /translation="MAEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFCCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDTSHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(47069..47827)
                     /gene="hyfI"
                     /locus_tag="ACHKA1_001390"
     CDS             complement(47069..47827)
                     /gene="hyfI"
                     /locus_tag="ACHKA1_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFO5679067.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDN
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLTGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHG"
     gene            complement(47824..48369)
                     /gene="hyfH"
                     /locus_tag="ACHKA1_001391"
     CDS             complement(47824..48369)
                     /gene="hyfH"
                     /locus_tag="ACHKA1_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO5679068.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(48379..50094)
                     /gene="hyfG"
                     /locus_tag="ACHKA1_001392"
     CDS             complement(48379..50094)
                     /gene="hyfG"
                     /locus_tag="ACHKA1_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO5679069.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTLEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGDSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALRIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(50084..51664)
                     /gene="hyfF"
                     /locus_tag="ACHKA1_001393"
     CDS             complement(50084..51664)
                     /gene="hyfF"
                     /locus_tag="ACHKA1_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO5679070.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRAVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGELGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(51669..52319)
                     /gene="hyfE"
                     /locus_tag="ACHKA1_001394"
     CDS             complement(51669..52319)
                     /gene="hyfE"
                     /locus_tag="ACHKA1_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO5679071.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(52331..53770)
                     /gene="hyfD"
                     /locus_tag="ACHKA1_001395"
     CDS             complement(52331..53770)
                     /gene="hyfD"
                     /locus_tag="ACHKA1_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFO5679072.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLLFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(53787..54734)
                     /gene="hyfC"
                     /locus_tag="ACHKA1_001396"
     CDS             complement(53787..54734)
                     /gene="hyfC"
                     /locus_tag="ACHKA1_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO5679073.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(54745..56763)
                     /gene="hyfB"
                     /locus_tag="ACHKA1_001397"
     CDS             complement(54745..56763)
                     /gene="hyfB"
                     /locus_tag="ACHKA1_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO5679074.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGTISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYLVAALVVLLIAIAV"
     gene            complement(56763..57380)
                     /gene="hyfA"
                     /locus_tag="ACHKA1_001398"
     CDS             complement(56763..57380)
                     /gene="hyfA"
                     /locus_tag="ACHKA1_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO5679075.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNENLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPQGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(57633..58103)
                     /gene="bcp"
                     /locus_tag="ACHKA1_001399"
     CDS             complement(57633..58103)
                     /gene="bcp"
                     /locus_tag="ACHKA1_001399"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO5679076.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(58103..58675)
                     /gene="gcvR"
                     /locus_tag="ACHKA1_001400"
     CDS             complement(58103..58675)
                     /gene="gcvR"
                     /locus_tag="ACHKA1_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO5679077.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            58821..59699
                     /gene="dapA"
                     /locus_tag="ACHKA1_001401"
     CDS             58821..59699
                     /gene="dapA"
                     /locus_tag="ACHKA1_001401"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO5679078.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            59716..60750
                     /gene="bamC"
                     /locus_tag="ACHKA1_001402"
     CDS             59716..60750
                     /gene="bamC"
                     /locus_tag="ACHKA1_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO5679079.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            60963..61676
                     /gene="purC"
                     /locus_tag="ACHKA1_001403"
     CDS             60963..61676
                     /gene="purC"
                     /locus_tag="ACHKA1_001403"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO5679080.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            61844..62707
                     /gene="ypfJ"
                     /locus_tag="ACHKA1_001404"
     CDS             61844..62707
                     /gene="ypfJ"
                     /locus_tag="ACHKA1_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO5679081.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            62722..64737
                     /gene="tmcA"
                     /locus_tag="ACHKA1_001405"
     CDS             62722..64737
                     /gene="tmcA"
                     /locus_tag="ACHKA1_001405"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO5679082.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARYGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSNAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            64811..65509
                     /gene="ypfH"
                     /locus_tag="ACHKA1_001406"
     CDS             64811..65509
                     /gene="ypfH"
                     /locus_tag="ACHKA1_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5679083.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(65590..65790)
                     /gene="ypfN"
                     /locus_tag="ACHKA1_001407"
     CDS             complement(65590..65790)
                     /gene="ypfN"
                     /locus_tag="ACHKA1_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO5679084.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(65818..66945)
                     /gene="dapE"
                     /locus_tag="ACHKA1_001408"
     CDS             complement(65818..66945)
                     /gene="dapE"
                     /locus_tag="ACHKA1_001408"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO5679085.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(66949..67305)
                     /gene="yffB"
                     /locus_tag="ACHKA1_001409"
     CDS             complement(66949..67305)
                     /gene="yffB"
                     /locus_tag="ACHKA1_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO5679086.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT        16306 a        16996 c        18004 g        16093 t
ORIGIN      
        1 aagcaccgta tcttcattgt gcggtccatg cgaagaccgc atcacgacag caccactggc
       61 gatattgccg cgatagcaag cactgtgggg tgcctgtatt tacggtcatg gaacatcctt
      121 tcctaaagcc agcgcccgtt tgcgagacgg cgaaaagggc cattaagaat aatactctta
      181 tgtttgaata attactctgt ttattatctt gacacagcca ggcataaaag agagtatttc
      241 aacactgtgg ggtgcctgcg ttgctccggc aacacaagtc aacctgctgg aaaggccgcg
      301 aaccagacca gcaataaaaa accgccaaat tcggcggttt tttattttcg gcggtttttt
      361 attgcctgta taaagcttat ttcaatggct caaccggtaa atctgcccgt gcgccccatt
      421 cactccacgc accgtcgtat aattttacgt ttggcacatc cagcgtcgcg agtgccaaca
      481 aaaccacggc tgctgttaca ccagagccgc agctgacgat aatcggtttg tcatagctga
      541 cgccgcgacc aaaaaatatc gcatccagtt catcggtcgt ttttagttcg ccttcgcgca
      601 ccagttccgt ccacggaacg ttcaatgcac cgggaatatg tccgcgacgt aaacctgggc
      661 gaggttcatc aacttctgcg ttaaaacgtg cagccgggcg ggcatcaata atttgcgccg
      721 tattttcatg gcttgccaat aacacatcgg ttactttcac cactgcttca ggattaaacg
      781 cggcgttaaa ctctccttcc ggcagctcta ctgcaccttc ttctaacaac agatcatcgc
      841 gctgccagcc tgcaagtcca ccccccagaa tcgacacttt ctctacacca aaggtgcgca
      901 gcatccacca tgctcgtggg gctgagaaaa gattaccttc gtcatagaca atcaggtgct
      961 tatcctggtt aacgcctaat tcacgcatcg ccacggcgaa cgtttccggg cgcggcagca
     1021 tgtgcggaag cggggaagtg tgatcagaaa gcgcttcgat atcaaaaaac actgcgccgg
     1081 gaatatgtcc attcagatac tcctgagcaa cgttacgatc ttcctgtcca ggcgacgcca
     1141 tgcgggcatc gataatctga atttccgggt catcaatatg ttcggcgagc cagtcggctc
     1201 ctacaaacca tgtcgtggac ataggcatct ccatttttgc cggggttagg ctaaattgtc
     1261 ggatgtttag tgaaaagcga caagcaaaag aggctgattt ggctgtctga gccacaattc
     1321 tcactaaaat tcggattgta tgggcgtgat actgaattac ataacgtaaa ttctcacgca
     1381 taataattat tcgacgttgc taacgggcca ttgtggcagg gatgaaaaat gaaaaagtta
     1441 cgcgtagccg cctgcatgct aatgctggcg ctggcagggt gcgacaacaa cgataacgcg
     1501 ccaacagcgg tgaaaaaaga tgcgccttct gaagttacta aagcggcctc ttcagaaaac
     1561 gcgagttcag caaaactctc cgtgccggag cgacaaaaac tggcacaaca gagtgccggt
     1621 aaggcgctga cattgctgga tctctctgaa gtccaacttg atggtgcagc cacgctggtg
     1681 ctgacgttct ccatccctct cgacccggat caggatttct cacgcgttat tcatgtcgtc
     1741 gataaaaaaa gcggcaaagt ggatggtgcc tgggagctgt cagataatct taaagagctg
     1801 cgtttacgcc acctcgaacc gaaacgtgat ttgatcgtta ctattggcaa ggaggtcaaa
     1861 gcactcaaca acgcaacctt cagtaaagat tacgaaaaaa ctataactac ccgcgacatc
     1921 caacccagcg tcggttttgc cagccgtggt tcgctgctgc ctggtaaagt cgttgaaggg
     1981 ctgccggtaa tggcgctcaa cgttaataat gtcgatgtta acttcttccg cgttaagcca
     2041 gaatctctgc cagcattcat tagccaatgg gaataccgca attcgctggc gaactggcag
     2101 tcagacaaac tgctgcagat ggcggatctg gtctacaccg gacggtttga tctcaatcct
     2161 gcgcgtaaca cccgtgaaaa attattgctg ccgctgggcg atatcaaacc gcttcagcag
     2221 gcgggcgtgt atctggctgt gatgaatcag gctggacgtt acgattacag taatcccgcg
     2281 acgctgttta cgttaagtga tatcggcgtt tcagctcacc gttatcacaa tcgtctggat
     2341 atctttaccc aaagtctgga aaacggcgcg gcccagcaag gaattgaagt ctctttatta
     2401 aatgagaaag ggcagactct gactcaggca accagtgacg ctcaggggca tgtgcagctg
     2461 gaaaatgata aaaacgccgc attattgctg gcacgtaaag acggtcagac aacgctactc
     2521 gatttaaaac ttccggcgct ggacttagca gaatttaaca ttgctggcgc gccaggctat
     2581 agcaaacagt ttttcatgtt tggcccgcgc gatctttatc gcccgggtga aacggtcatc
     2641 ctcaatggtt tgctgcgtga tgcagacggt aaagcgttgc ccaatcaacc catcaagtta
     2701 gaagtgatta aacccgatgg gcaggtactc aggagcgtcg ttagtcagcc ggaaaatggc
     2761 ctctaccact ttacctggcc actcgatagc aatgcggcaa ccggtatgtg gcatattcgc
     2821 gctaacacgg gcgataacca gtatcggatg tgggatttcc acgtcgaaga ttttatgcca
     2881 gagcgtatgg cgctgaatct gaccggtgag aaaaccccgc ttacgccgaa tgatgaagtg
     2941 aaattctccg tggtgggata ctacctgtac ggtgcacctg ctaatggtaa tactttgcaa
     3001 gggcaacttt tcctgcgccc actgcgtgag gctgtgtcag ccttacctgg ttttgaattc
     3061 ggcgatatag ctgccgaaaa cctttcccgc acgctggatg aagttcagtt gacgctggat
     3121 gataaagggc gcggcgaagt ttctacagaa agccagtgga aggaaacgca ttccccatta
     3181 caggttattt tccagggcag tttgctggaa tcgggcggtc gcccagtgac gcgtcgcgct
     3241 gagcaggcta tctggcctgc cgatgcattg ccggggatcc gtccgcagtt cgcctcgaaa
     3301 tcggtttacg attatcgcac tgacagcacg gtgaaacagc ccattgttga tgaaggcagt
     3361 aacgccgctt ttgacatcgt ttatagcgat gcgcaaggcg tgaaaaaagc cgtgtcgggc
     3421 ttgcaggtgc gcctgattcg cgaacgccgc gattactact ggaactggtc agaagatgaa
     3481 ggctggcaat cacagtttga tcaaaaagat ctgattgaaa atgaacaaac tctggatctg
     3541 aaagcggacg aaaccggcaa ggtcagtttc ccggtagagt ggggcgctta tcgtctggaa
     3601 gtcaaagcgc cgaatgaagc ggtcagtagt gtgcgtttct gggctggcta tagctggcag
     3661 gacaacagcg acgggagcgg tgccgtgcga cccgaccgtg tcacgctgaa actagataaa
     3721 gccagttatc gccctggcga taccattaag ttgcatattg ccgcgccaac ggcgggtaaa
     3781 ggttatgcga tggtcgagtc cagtgaaggg ccgctgtggt ggcaagagat tgatgttccg
     3841 gctcaagggc tggatctgac gattccggtc gataaaacct ggaatcgtca tgatctttat
     3901 ttgagtacgc tggtggtgcg tcctggcgat aaatctcgct ccgcgacgcc aaaacgcgcg
     3961 gttggtgtgt tgcatctgcc gcttggtgat gaaaaccgtc gcctcgatct ggcgctggaa
     4021 acaccagcaa aaatgcgtcc caatcaacca ttaaccgtga aaattaaagc cagcactaaa
     4081 aatggcgaga agcctaaaca ggtgaatgtg ctggtgtctg ccgttgatag tggtgtgctg
     4141 aatattactg actacgtcac gccagatccg tggcaggcgt tctttggtca gaaacgctat
     4201 ggcgcagaca tttacgatat ttacggtcag gttattgaag gtcaggggcg tctggcagct
     4261 ctgcgtttcg gtggcgatgg tgatgagctg aaacgtggtg gtaaaccgcc ggtcaatcac
     4321 gtcaatattg tcgcgcagca ggcgctgccg gtaacgctca acgaacaggg cgaaggctcg
     4381 gttacactgc cgattggcga ttttaacggt gaattgcgcg tcatggcgca agcctggacg
     4441 gcagatgact tcggtagcaa cgaaagcaaa gtgatagttg ccgcaccggt gattgctgaa
     4501 ctgaacatgc cgcgctttat ggcgagtggc gatacctcgc gtctgacgct ggatatcact
     4561 aatcttaccg ataaaccgca aaaactgaac gttgccctga ccgccagtgg tttgcttgaa
     4621 ctggtcagcg attcacccgc agccgttgaa ttagcgccag gtgtgcgtac tacgctgttt
     4681 atcccggtgc gagcattgcc gggttatggc gatggagaaa ttcaggccac cattagcggg
     4741 ttagcgttac cgggtgaaac cgttgccgat cagcataagc agtggaaaat cggcgtccgt
     4801 ccggcgttcc cggcacaaac ggttaattac ggtacggcgt tacagcctgg tgagacatgg
     4861 gcgattccgg cggatggatt gcaaaacttc tcacctgtta cgctggaagg gcaattgttg
     4921 ttgagcggca aaccaccgct gaacatcgca cgttatatca aagagttaaa agcgtatccg
     4981 tacggctgtc ttgagcaaac cgccagcggc ctgttcccgt cactttatac caacgcagcc
     5041 caactgcagg cgttgggcat caaaggcgac agtgatgaga aacgccgtgc atcggtcgat
     5101 atcggcattt cccgtttgct gcaaatgtaa cgtgataacg gcggctttgc gctgtgggat
     5161 aaaaacggtg acgaagagta ctggctgacg gcttacgtga tggatttcct ggtccgcgca
     5221 ggcgaacagg gttacagcgt gccgacagac gccattaacc ggggtaatga gcgtctgctg
     5281 cgctatttac aagatccggg catgatgtcg atcccgtacg cggataatct caaagccagt
     5341 aaattcgccg tacagtcata cgctgcgctg gtgttggccc gtcagcaaaa ggctccgctg
     5401 ggtgcgctgc gtgaaatctg ggagcatcgt gcagatgccg cttctggttt accgctgctg
     5461 caacttggcg ttgcgctgaa aaccatgggt gatgcgacgc gttgtgaaga agcgattgcg
     5521 ctggcgctaa aaacgccgcg taatagtgat gagcggatat ggctgggtga ttacggtagt
     5581 ccactgcgcg acaacgcgtt gatgctctcc ttgctggaag agaacaaact gctacccgat
     5641 gagcagtact ccctgctgaa cacactttcg cagcaggcgt ttggtgaacg ctggctatcg
     5701 acgcaggaaa gtaacgcgtt gttcctggct gcccgtacga ttcaggattt accaggtaaa
     5761 tggcaggcgc aaacctcttt ctcaactgag ccgctgacag gcgagaaagc gcaaaacagc
     5821 aatctgaata gcgatcaact tgccaccttg caggtgacca acagtggcga gcagccgtta
     5881 tggctgcgtg tggatgccag cggttatccg caatccgcac ctttaccggc gaacaatgtg
     5941 ctgcaaattg agcgtcatat tcttggtact gatggtaaga gcaaatcgct ggactcgtta
     6001 cgtagcggcg atctggtgct ggtctggttg caggtaaaag ccagtaacag cgtgccggat
     6061 gcgttagtcg tggatctgct gcctgcgggt ctggaactgg aaaaccagaa tctggcgaac
     6121 ggtagcgcca gcctggagca aagtggtggc gaagtgcaga acttactgaa ccagatgcag
     6181 caggcgagta ttaagcacat tgagttccgt gacgatcgct ttgtggcggc ggttgccgtt
     6241 gatgaatacc aaccggtaac gctggtgtat ctggcgcggg cggtgacgcc gggaacgtat
     6301 caggtaccgc aaccgatggt ggaatcaatg tatgttcccc aatggcgggc gaccggcgcg
     6361 gctgaagatt tgctgattgt cagaccgtaa atgcctcgct tgttaaccaa acgcggctgc
     6421 tggataacgc tggcagctgc gccatttctc ctttttcttg cagcgtgggg agcagataaa
     6481 ctctggcctc taccgctgca tgaagtcaat cccgcacgag tggtcgtggc gcaggatggt
     6541 acgccgctct ggcgcttcgc cgatgctgac ggcatctggc gttatccggt aacaatcgaa
     6601 gatgtttctc cacgttacct tgaagcgctg atcaattatg aagatcgctg gttctggaag
     6661 catccggggg tgaatccatt ctcggtggcg cgcgcagcat ggcaagatct cacttcggga
     6721 cgggttattt ccggtggcag cacgctcact atgcaggttg ctcgtctgct tgatcctcac
     6781 cccaaaacat ttggcggcaa aattcgccag ctctggcgcg cgttgcaact ggaatggcat
     6841 ctgtctaagc gtgaaattct gaccttgtat cttaaccgcg ctccgtttgg cggtacgttg
     6901 caggggatcg gtgcggcaag ttgggcttat ctcggaaagt cgcctgcgaa tttaagctat
     6961 tccgaggcgg caatgctggc ggttttgccg caagcgccca gccgtcttcg cccggatcgt
     7021 tggccggagc gtgccgaagt cgcgcgtaat aaagtgctcg aacggatggc cgtgcaaggt
     7081 gtgtggtccc gtgagcaggt aaaagagtca agggaagaac ccatctggct ggcaccccga
     7141 caaatgccgc aactggcacc gctgttttcg cgcatgatgc tcggtaaaag caaaagcgac
     7201 aaaatcgtta ctacgctgga tgccggtctt caacgacgtc tggaagaact ggcgcaaaac
     7261 tggaaaggac gattaccacc gcgcagctca ctggcgatga tcgtggttga tcataccgat
     7321 atgcgtgttc gcggctgggt gggttcggtt gatctcaacg atgattcacg ctttggtcat
     7381 gttgatatgg tcaatgcgat ccgatcgcca gggtcggtgc tcaaaccgtt tgtttacggt
     7441 ctggcgctgg atgaaggctt gatccatccg gcatcgctgt tgcaagacgt cccccggcgc
     7501 accggtgatt atcgaccagg taactttgat agcggttttc atggcccgat cagcatgagc
     7561 gaggcgctgg tgcgctcgct gaatttacct gctgtgcagg tactggaagc ctatggaccg
     7621 aaacggtttg cggcaaagtt acgcaatgtt ggattgccgt tatatttgcc caacggtgcg
     7681 gcgccgaatc tttcgctcat tctcggcggc gctggtgcaa aactggaaga tatggcggca
     7741 gcgtataccg cgtttgctcg ccagggcaag gcaggcaaat tgcgcttaca gcctgacgat
     7801 ccgctgcttg aacggccttt aatgtcgtcg gaggctgcgt ggatcattcg ccggattatg
     7861 gctgatgaag cgcaaccctt gccggatggt gccttgccgc gcgtcgcccc actggcatgg
     7921 aaaacgggca ccagctatgg ctatcgtgac gcctgggcga ttggggttaa cgctcgctat
     7981 gtcattggga tctggactgg cagaccggac ggcacgcccg ttgttggtca gtttggcttt
     8041 gccagtgccg taccattgtt gaatcaggtc aataatatct tactgtcgcg cagtgctaat
     8101 ctgccggaag acccgcgccc gaactcagtc actcgtggcg ttatttgctg gccgggcgga
     8161 cagtctttgc cggaaggtga cggtaactgt cgccgccgcc tggcaacctg gctgctggac
     8221 gggagtcagc cgccaactct attactgccg gagcaggaag gcattaatgg cattcgtttc
     8281 cccatctggc tggatgaaaa tggcaaacgt gttgccgctg attgccctca ggcgcaacaa
     8341 gaaatgataa atgtctggcc gctaccgctg gaaccctggt tgcccgcatc agaacgtcgc
     8401 gctgtgcgct taccaccggc ctcgacaatt tgcccgccat acggtcacga tgcccagctt
     8461 ccgctgcagc tgacaggcgt tcgcgatggc gagattatta aacgtttacc gggcgcagcg
     8521 gaagcgactt tgccgttgca atcaagtgga ggggcaggtg aacgctggtg gtttctgaat
     8581 ggcgaaccgt taactgaacg cgggcgcaac gtgaccctgc atttgacgga taaaggtgat
     8641 tatcaattac tggtgatgga tgacgtaggg caaatcgcgt cagtacattt ttcatttcag
     8701 tgaaatatac aaaatattta ttaatctaat tatggtattt atatttggaa tgcattaata
     8761 atttaataat ctcacgtttt tttaaacgac taattatttg attgttatca gcattgttct
     8821 cgaataaata cgtgaatatt tattttttca ttttcgggaa cgcaagatgg atacgccaga
     8881 cgaaaaggaa cgcattacat ttccgcaagt atcacgacgt agttttttac aagcgacttc
     8941 cgcgcttatc actttgcctt ttatttcttc taccgcaaag gcgcaatccc cagatgcgtc
     9001 gccagaagtt acagctccgg tggctgataa agtcgtgccc acctgtagca catttgactg
     9061 cggtggtaag tgcgatattc gggcgcatat gcgcgacggt gtggtgacgc aaattaccac
     9121 gcttcccgat aacgaactgg atccacaaat gccgattatg cgcgcctgtg ttcgtggtcg
     9181 cggctatcgt aaatttgttt atcatccaga ccgtttgaaa tatccgatga agcgggtagg
     9241 taaacgaggc gagggtaaat ttgagcgtat ttcatgggat gaggcgacga ccttaattgc
     9301 cgataacctg aaaaggatca cgcaacaata tggcccggct tcacgttatg tccatgtcgg
     9361 gacggctgtt tctggtggaa cattttctgg cgatgcgatg gcgcgtcggc tgttaaattt
     9421 aaccggtggt tatcttgaat attatcactc agttagtctg ggtaatactg cggcggcaac
     9481 gccttacacg tatggcgtgg cggccagcgg taactcgatg gatactcttc tggataccaa
     9541 actggtgatc ctctgggggc ataacccaac ggaaaccatc ttcggtcaca ccaattactt
     9601 ttttcaaaaa atgaagcaaa acggtacgcg ttttatcgtg gtagatccgc gttattccga
     9661 cactgtatct tcgcttgccg atcagtggat cccgctgctg ccgaccacgg acaatgcgtt
     9721 gatggacgcg atgatgtacg tcattatcag cgagaatctc cacgataaga catttatcga
     9781 cacatatact ctcggttttg atgaaaactc tatgccagaa ggcgtaccag ccaacgaatc
     9841 gttggttgcc tacctttttg gcgcaaaaga tgggatacac aaaacgccgg aatgggcaga
     9901 aaaaattacc cacgttccag cacaatccat tcgtcaactg gcacgcgatt acgccactac
     9961 aaaacccgca gcgttgattc agggatgggg gccacagcga catacttgcg gcgaacgcac
    10021 cgcccgaggt tcaactttac tggcctcgat taccggtaac gtcggcatca aaggtggctg
    10081 ggcggcaggt tacggcggta gttctaatcg taaattctgc gttggaccag atatgccaga
    10141 aaatccggta caagcgaaaa tctcgattat gaactggatg caggcggcgg atgatgcttc
    10201 taaagttacg ccgcaagatg ggcttaaagg tgtcgataaa ctcgacagta atatccgcct
    10261 gctgttctca ctggcaggta attatctggc gaaccagaac ccggacgtcc accaggccgc
    10321 gaagctcctt gaagacgaat ctaaaataga attcatcgta ctcagcgatc tgttcatgac
    10381 tccgagcgcg aaatatgccg atgtgttgct gccggaaacc agctttatgg aacgctggaa
    10441 tatcggcgaa acatggggaa ctgcgagcta tcttattctg tcagaaaaac tgattgagcc
    10501 ggattttgaa cgtcgcaccg attatgactg gttgcgtgac gtggcgaaaa aactgggtgt
    10561 cgaagccgag ttcagtcaag gacgtgatga gaagcaatgg atcgagcata tctgggagca
    10621 aactcgtctg gcgatgccgg atgaaaattt gccggacttc gcgaccctgc aaaaaacacg
    10681 tcgacatctt ttcaaaagcg cgccgcacat cgctttcgaa gcaaatattc gcgatccgca
    10741 gaataatcct ttcccgacgc cttccggcaa aattgagatc ttctctaagc gtctttttga
    10801 tatgcaagat ccagaaattc ccgcgctttc gcactatgtc cctgcctttg aagggccaga
    10861 agataaactg acggcgaaat acccgctgca gctcatcacc tggaaaggga aaaaccgtgc
    10921 taattccacc cagtatgcta acccgtggct gcaagaagtt cagacgcaaa aactctggct
    10981 gaatccgcaa gatgcaaaac agcgcgggat tgtcgaagga gatagcgtaa aaatttataa
    11041 cgatcgtggt gtgagcatta tcccggtgga aataaccccc cgtatcatcc ctggcgtagt
    11101 tgctatgcag gcgggggcat ggtggcaacc ggatgcacaa ggtatcgatc gcggaggctg
    11161 tgccaatgta ctgagttcga cccgcattac ggcgctggcg aaagggaatt cgcatcaaac
    11221 aatgctggtg gaggttgaga aagcatgagc caatttaaag aatttgcatc tgtcagcgac
    11281 aaacaattag ggttctttat tgactcttcg cgttgttcgg gctgtaaagc gtgtcaggtg
    11341 gcctgtaaag acaaaaacaa ccttgaggtt gggcgacgtt ttcgccgcgt gtatgaagtg
    11401 aacggaggtg gcttcatcgc cactggagag ggcggtgtga gtcataatgt ctttgcttat
    11461 accctttctg tttcctgtaa ccattgcgcg gatccgatct gtaccaaaaa ttgcccgacg
    11521 atggcgatgc ataagcgtcc cggagacggc attgtgcgcg tgaatactga taagtgcgtc
    11581 ggttgcggtt actgcgcttg gtcctgtcct tacggcgcac cacaaatgaa tgagcagacc
    11641 gggcagatgt cgaaatgcga tttctgtatt gatctgcaag ctaaaggaga acaacccatt
    11701 tgcgtggcaa cgtgcccgtt gggggcgatt aagttcggcc ctattgatga gttgcgtgaa
    11761 aagtatggcg atgtcagtga tgtgaaaggt ttacctgatt cgtcgattac gcagccaaat
    11821 ctggtgatta aaccgcatca gggcgcggaa aaggaggcgt cacatcatgc atgaattacc
    11881 gttaatggct tttacgctgt tgttacaggc atcggtcggt atgacgttgt ggctggcgtt
    11941 tcttcatcgt catattttaa tcacctccag cgcccccccg gttatgcggc cgccattact
    12001 gttggctttt ttggcagggg ccgtagggtt actgatttct accttacacc tgggttatcc
    12061 acttaatgcg atgcatgcgc taagtcactt ttccagttca tggctgagtc gggaaattat
    12121 ctttggtgcg ctgtatctgg cgctattggg tttatccaca ttactggtca tgttacgcaa
    12181 aggcggctgg caactcctgc ttatgctagc ggcagtggtc gggattgttg atgttttctg
    12241 catggcgcaa gtttacatcc atacgtcgat tatcacctgg cagcattaca acacgctgat
    12301 tatgttctta ggtacagtgg gaattctcgg ttcagcgctg gtagcagtgt ttcgtattag
    12361 cggaattttg ccgcagattg atgccctgcg taatggatgc gtgttggtga tcgctctcct
    12421 ggttctgctg cgtttgttgg ttcagccgtt gtggataggg gatttaacgg caaatgcgat
    12481 gcagatagcc acattgccac atgcgccatt agcgatgctg ggacagttaa aacccatact
    12541 gacactaagc tggggaattt ccgtcattgg gatgatgttc tttgctgtcg gtggttgtaa
    12601 aaagaatatc ccggcggcgt tattcggtag cgtaatgcta gtggggagtg aagttatgct
    12661 gcgttttgtc ttttttagta tcggctaatg ttaagtcata acataacgat tggcgcggaa
    12721 tgcgcgcgcc aacgctttcg tttcgcaaca tgtcatgcct gtgtcgaggc ttgcccggca
    12781 caggcgctaa cgctgacaga agagggcata gcggtcgacg cggaacaatg tattgagtgt
    12841 gcggtttgcc agtttatttg tccacaagag gctattcgtg gcgttaattc ccctttacgt
    12901 tattttcgca atcacaccct ggtcgcacct ttcaccacta ttgcgccgac gctggaagaa
    12961 cttttgctat ggcataaaga gcaggggatc acagcgatcg cgattgatct gagtcaggcg
    13021 aaggcatggg agcgcgcatt attgctgtta aacgaacgct tacggcaata tggcgaggaa
    13081 ggctggtctg tggtatctcc acataccagt gaggtgaatg tttcccggcg tcgtttgttc
    13141 catgtgcagc gtgcggatgt tcagatgaca caaatacaac caggattacg tcggttacat
    13201 tatttatggc cccagattag tgatgctcgc ccgatgatgg atcctcaaaa gtgtcagctt
    13261 tgcggtgcat gctggcgggc ctgcgagcaa caggtctttt cgcttaacga aggccattta
    13321 caaataaatg atgccttgtg taacggatgc caaaattgta ttgctgtttg ttttcatcag
    13381 gcaatgaccg ttgaattgac gattctaccg gcaaaaattg tgaatcttca tgctaatcgc
    13441 aaagtctgta aaacctgtca aaaatccttt cttacttttc agcaaaatgc gcaaaattgt
    13501 ctttattgcc aacgtcatcg ctatgggatg agagcaccat aaaagtcgag ttgttgctaa
    13561 aactcatttt attttaaaaa aatgttacct tcatcaatag tcaacggccc tgttgctctt
    13621 tataatccgc gccatctcgt acgcaggtac agacaacaac gaatacttta cagaggtaaa
    13681 aatggctatt gaacgtactt tttccattat caaaccgaac gcggtagcaa aaaacgtcat
    13741 tggtaatatc tttgcgcgct ttgaagctgc agggttcaaa attgtcggca ccaaaatgct
    13801 gcacctgacc gttgaacagg ctcgtggctt ttatgctgaa cacgatggaa aaccgttctt
    13861 tgatggtctg gttgaattca tgacctctgg cccgatcgtg gtttccgtgc tggaaggtga
    13921 aaacgccgtt cagcgtcacc gcgatctgct gggcgcgacc aatccggcaa acgcactggc
    13981 cggtactctg cgcgctgatt acgctgacag cctgaccgaa aatggtaccc acggttctga
    14041 ctccgtcgaa tctgccgctc gcgaaatcgc ttatttcttc ggcgaaggcg aagtgtgccc
    14101 gcgcacccgt taataatttc gtaaatgccg cgtgcaaacg tggcatccgt gcgccagaat
    14161 ttgtacaatg cagcgccccc ggacgagcag ccgctcaccg gggcgtttct tttttcaacc
    14221 ctccaggggc cataacgtgt aataacgagg ccggaatagc atatgtctga acaattagtc
    14281 acacctgaaa acgtcaccac caaagatgga aaaatcaacc tgctggatct caatcgtcag
    14341 cagatgcggg agttttttaa agatttaggt gaaaaaccct tccgcgccga tcaggtgatg
    14401 aagtggatgt atcactattg ctgcgacaat tttgatgaga tgaccgacat caacaaagtg
    14461 ttgcgcggca aactgaaaga ggtggcggag atccgcgcac cggaagtggt tgaagaacag
    14521 cgttcatctg acggcaccat taaatgggcg atcgccgtag gcgatcagcg cgtcgaaacg
    14581 gtgtatatcc ctgaagacga ccgtgccacg ctctgcgtct cttcgcaggt ggggtgtgcg
    14641 ctggagtgta aattctgttc caccgcccag cagggcttta accgcaacct gcgggtgtcg
    14701 gaaattatcg gccaggtgtg gcgtgcggcg aaaatcgtcg gcgcagcaaa agtcaccggt
    14761 cagcgcccga tcactaacgt agtgatgatg ggcatgggcg agccgctgct caacctgaac
    14821 aacgtcgttc cggcgatgga aatcatgctt gatgatttcg gttttggtct gtctaaacgt
    14881 cgcgtcacgc tttccacttc cggtgtagtt ccggcgctgg ataaactggg cgatatgatc
    14941 gacgttgcac tggcaatttc cttacacgcg ccgaacgacg aaattcgtga tgaaattgtg
    15001 ccgatcaaca aaaagtacaa tatcgaaacg ttccttgctg cggtgcgccg ttatctggag
    15061 aaatccaacg ccaatcaggg ccgagtcact attgaatacg tgatgcttga ccacgttaac
    15121 gacggcactg aacacgcgca ccaactggcg gaactgctga aagatacgcc gtgtaagatc
    15181 aacctgatcc cgtggaaccc gttcccgggt gcgccgtatg gacgcagctc gaacagccgt
    15241 atcgaccgtt tctcaaaagt gctgatgagc tacggtttca ccactattgt gcgtaaaacg
    15301 cgtggtgatg atatcgatgc tgcctgtggt cagttggcgg gcgatgttat cgaccgtacg
    15361 aaacgtacac tgcgtaaacg gatgcagggt gaagccatcg acattaaagc ggtctgatag
    15421 ccattacgcc acggttactt tatgtaccgt ggcgtaatgt tttatgaatg aagtattaag
    15481 ttgcaattgt ctggtcaata ttaacggtgc gtttttgctg actttaaggc agtatgtaac
    15541 gatgcaacag taagttagcc ttagtgaatg tgggctttgt cacgagcaca cagacggtct
    15601 tatactgtat gataacggtt aacttaacgg atgtttcgcg gtgtgggtga gcatgatggt
    15661 tcaccggcat ctcaattctc atttaaacgt acctgcagcg aatgaatact gaagccacgc
    15721 acgaccaaaa tgaagcactt actaccggcg ctcgcctgcg taatgctcgc gaacaactag
    15781 gacttagtca gcaggccgtt gccgagcgac tttgcctgaa ggtttccacg gtacgcgaca
    15841 ttgaagaaga taaggcaccc gccgatcttg cttcaacatt cctgcgcgga tatatccgct
    15901 cttatgcgcg tctggtacat attccagaag aagaactgct gccagggctg gaaaagcagg
    15961 ctccactacg ggctgcaaaa gttgcgccga tgcagagttt ttccctcggt aaacgccgca
    16021 aaaaacgcga cggctggctg atgaccttca cctggctggt gttgtttgtg gttatcggcc
    16081 tgagcggtgc ctggtggtgg caagaccaca aagctcagca ggaagagatc accactatgg
    16141 ccgatcaatc ttcggcggaa ctgagcagta atagcgagca ggggcagagt gttccgttaa
    16201 atacgtcgac aactacagac ccggctacaa cgtccacgcc gccagcgtct gtggatacta
    16261 ccgcaaccaa cacgcaaaca cctgccgtaa ctgcgccagc accagctgtt gatccgcaac
    16321 agaatgcggt tgtttcgcct tcgcaggcaa atgttgatac cgccgcgacc ccggcaccaa
    16381 cggcagcaac aacgccagat ggtgctgcgc cgttgccaac cgatcaggct ggcgtgacca
    16441 cgccggtggc tgatccgaat gcgctggtga tgaactttac tgccgattgc tggctggagg
    16501 tcactgatgc taccggtaaa aaattgttta gcggtatgca gcgtaaagac ggtaatttga
    16561 acttaaccgg ccaggcacca tacaaactga aaattggtgc gccagccgca gtacagatcc
    16621 agtatcaagg gaaacctgtc gatctgagtc gttttatcag aactaaccag gttgcgcgtc
    16681 tgaccctcaa tgccgaacaa tcaccggcgc agtaacagac gggtaacgcg ggagattttt
    16741 catgcataac caggctccaa ttcaacgtag aaaatcaaca cgtatttacg ttgggaatgt
    16801 gccgattggc gatggtgctc ccatcgccgt acagtccatg accaatacgc gtacgacaga
    16861 tgtcgaagca acggtcaatc aaatcaaggc gctggaacgc gttggcgctg atatcgtccg
    16921 tgtctccgta ccgacgatgg acgcggcaga agcgttcaaa ctcatcaaac agcaggttaa
    16981 cgtgccgctg gtggctgaca tccacttcga ctatcgcatt gcgctgaaag tagcggaata
    17041 cggcgtcgat tgtctgcgta ttaaccctgg caatatcggt aatgaagagc gtattcgcat
    17101 ggtggttgac tgtgcgcgcg ataaaaacat tccgatccgt attggcgtta acgccggatc
    17161 gctggaaaaa gatctgcaag aaaagtatgg cgaaccgacg ccgcaggcgt tgctggaatc
    17221 cgccatgcgc catgttgatc atctcgatcg cctgaacttc gatcagttca aagtcagcgt
    17281 gaaagcgtct gacgtcttcc tcgctgttga gtcttatcgt ttgctggcaa aacaaatcga
    17341 tcagccgctg catctgggga tcaccgaagc gggtggcgcg cgcagcgggg cagtaaaatc
    17401 cgccattggt ttaggtctgc tgctgtctga aggcatcggc gacacgctgc gcgtatcact
    17461 ggcggccgat ccggtcgaag agatcaaagt cggtttcgat attttgaaat cgcttcgtat
    17521 ccgttcgcga ggtatcaact tcatcgcctg tccgacctgt tcgcgtcagg aatttgatgt
    17581 tatcggtaca gttaacgcgc tggagcaacg cctggaagat atcatcactc cgatggacgt
    17641 ttcgattatt ggctgcgtgg tgaatggccc gggtgaggcg ctggtttcta cactcggcgt
    17701 caccggcggc aacaagaaaa gcggtctcta tgaagatggc gtgcgcaaag accgtctgga
    17761 caacaacgat atgatcgacc agctggaggc acgcattcgt gcgaaagcca gtcagctgga
    17821 cgaagcgcgt cgaattgacg ttcagcaggt tgaaaaataa taacgtgatg ggaagcgcct
    17881 cgcttcccgt gtatgattga acccgcatgg ctcccgaaac attgagggaa gcgttgaggg
    17941 ttcattttta tattcagaaa gagaataaac gtggcaaaaa acattcaagc cattcgcggc
    18001 atgaacgatt acctgcctgg cgaaacggcc atctggcagc gcattgaagg cacactgaaa
    18061 aacgtgctcg gcagctacgg ttacagtgaa atccgcttgc cgattgtaga gcagaccccg
    18121 ctattcaaac gtgcgattgg tgaagtcacc gacgtggttg aaaaagagat gtacaccttt
    18181 gaggatcgca atggcgacag cctgactctg cgccctgaag ggacggcggg ctgtgtacgc
    18241 gccggcatcg agcatggtct tctgtacaat caggaacagc gtctgtggta tatcgggccg
    18301 atgttccgtc acgagcgtcc gcagaaaggg cgttatcgtc agttccatca gttgggctgc
    18361 gaagttttcg gtctgcaagg tccggatatc gacgctgaac tgattatgct caccgcccgc
    18421 tggtggcgcg cgctgggtat ttccgaacac gtaactcttg agctgaattc tatcggttcg
    18481 ctggaagcac gcgccaatta ccgcgatgcg ctggtggcat tccttgagca gcataaagaa
    18541 aagctggacg aagactgcaa acgccgcatg tacactaacc cgctgcgcgt gctggattca
    18601 aaaaatccgg aagtgcaggc gcttctcaac gacgctccgg cattaggtga ttatctggac
    18661 gaggaatctc gtgagcattt tgccggtctg tgcaaactgc ttgagagcgc ggggatcgct
    18721 tacaccgtaa accagcgtct ggtgcgtggt ctggattact acaaccgtac cgttttcgag
    18781 tgggtgacta acagtctcgg ctcccagggc accgtgtgtg caggcggtcg ttatgacggt
    18841 cttgtggaac aactgggcgg ccgtgcaaca ccggctgtcg gttttgcgat gggcctcgaa
    18901 cgtcttgtat tgttagtaca ggccgttaat ccggaattta aagccgatcc tgttgtcgat
    18961 atatacctgg tggcttcagg tgctgataca caatctgcgg ctatggcatt agctgagcgt
    19021 ctgcgtgatg aattaccggg cgtgaaattg atgaccaacc acggcggcgg caactttaag
    19081 aaacagtttg cccgtgctga taaatggggt gcccgcgttg ctgtggtgct gggtgagtct
    19141 gaagtggcta acggcacagc agtagtgaag gatttgcgct ctggtgagca aacggcagtt
    19201 gcgcaggata gcgtagccgc gcatttgcgc acgttactgg gttaaggaag gagaaggaca
    19261 gcgtggaaat ttacgagaac gaaaacgacc aggtagaagc ggttaaacgc ttttttgctg
    19321 aaaatggcaa agcactggct gttggggtga ttttgggcgt tggcgcactg attggctggc
    19381 gctactggaa cagccatcag gttgattctg cacgctccgc ttctcttgcc tatcaaaatg
    19441 cggttaccgc agtgagcgaa ggcaaaccgg atagcatccc ggcggcggaa aaatttgctg
    19501 ctgaaaataa aaatacttat ggtgcgctgg cttctttgga acttgcgcag caatttgttg
    19561 acaaaaatga actagagaaa gctgccgccc agttacaaca ggggctggca gacacgagcg
    19621 atgaaaatct caaagccgtg ataaatctgc gtcttgctcg cgttcaggta cagctcaagc
    19681 aggctgatgc cgcgctgaaa acccttgata ccatcaaagg tgaagggtgg gctgccattg
    19741 ttgccgacct gcgtggtgaa gcattgctga gcaaaggtga taagcaaggt gcgcgtagtg
    19801 catgggaagc aggcgtgaaa agcgatgtta ctccggcact gagcgaaatg atgcagatga
    19861 aaattaataa tttgtccatc tgagagggac ccgatgcaat tgcgtaaatt actgctgcca
    19921 ggactgcttt ccgttaccct tttaagcggc tgttcgctgt ttaacagcga agaagatgtg
    19981 gtaaagatgt ccccattgcc aaccgttgaa aaccagttta cgccgaccac ggcgtggagc
    20041 acttccgttg gtagcggcat tggcaacttc tattccaatc ttcatccggc actggcggac
    20101 aacgttgtct atgcagcgga ccgcgctggt ttagtaaaag cgctgaatgc ggatgatggc
    20161 aaagaaatct ggtctgtcag cctggccgag aaagatggct ggttctctaa agagcctgca
    20221 ttactttctg gcggtgtgac cgtgtctggt gggcatgtct acattggcag cgaaaaggcg
    20281 caggtttacg cgctgaatac cagcgatggt actgtggcat ggcaaactaa agtcgcgggt
    20341 gaagcacttt cgcgcccggt ggtcagcgac ggtctggtgt taatccacac cagtaacggt
    20401 cagttacaag cgctgaacga agctgacggc gctgtcaaat ggacagttaa cctcgatatg
    20461 ccttcgctct ctttgcgtgg cgagtctgcg ccggcaacgg cttttggtgc ggccgtcgtg
    20521 gggggcgata atggtcgcgt cagcgcagtg ctgatggaac agggccagat gatttggcag
    20581 cagcgtattt cccaggcgac cggttctacc gaaattgacc gtctgagcga tgttgacacg
    20641 actcccgtcg ttgttaacgg cgttgttttc gcgctggcct ataatggtaa cctgacggcg
    20701 cttgatctgc gcagtggtca gattatgtgg aaacgcgaac tgggttcggt gaatgatttc
    20761 atcgtcgacg gcaatcgcat ctatctggtc gatcaaaatg accgggtgat ggcgttgacc
    20821 attgatggcg gcgttacgct gtggacacaa agcgatctgc tgcatcgcct gctgacttct
    20881 ccggtgctgt ataatggcaa cctggtggtc ggtgacagtg aaggttattt gcactggatt
    20941 aacgtcgaag atggtcgttt cgttgcccag caaaaagttg atagttccgg tttccagact
    21001 gaaccggttg ccgctgacgg caaactgctg atccaggcaa aagacggaac cgtgtactct
    21061 attacacgtt aatcgtctct gtcgttcact ttgaaaacgg ctcctggaca ggggccgttt
    21121 tcctgttttt aacaacgacg cgaatatagc gtgcgttgtc tgatgattta taaaaatgag
    21181 gctttaaaca tggtacctgt ggtcgcgctt gtcgggcgcc ctaacgtagg aaaatccacg
    21241 ttatttaacc gtctaactcg cacccgagat gcgctggttg cggatttccc gggtctgact
    21301 cgtgaccgta agtacggtcg tgcggaaatt gaaggccgtg agtttatctg tattgatacc
    21361 ggcgggattg atggcacaga agacggtgta gaaacccgca tggcggaaca gtcgctgctg
    21421 gcgattgaag aagcggacgt cgtactgttt atggtggatg cgcgcgcggg cctgatgccg
    21481 gcagatgaag cgattgccaa acatctgcgc tcccgtgaaa aaccgacctt cctggtggca
    21541 aacaaaactg acggtctgga tcccgatcag gcagtggttg atttctactc gcttggttta
    21601 ggtgaaatct acccgatcgc cgcgtctcac ggtcgtggcg tattaagtct gctggagcat
    21661 gtgctactgc cgtggatgga agatctcgca ccgcaagagg aagtcgacga agacgctgaa
    21721 tactgggcgc aatttgaagc ggaagagaac ggcgaagaag aagaggaaga cgacttcgac
    21781 ccgcaaagtc tgccgatcaa actggcgatt gtgggtcgtc cgaacgtagg taagtctaca
    21841 ctcactaacc gtattcttgg tgaagagcgc gttgttgttt atgacatgcc tggtacgacg
    21901 cgtgacagta tctacattcc aatggaacgc gatggacgtg agtatgtgct cattgacacc
    21961 gctggcgtac gtaaacgcgg caaaatcacc gatgctgtag agaaattctc cgtaatcaaa
    22021 acgttgcagg ccattgaaga cgccaacgtg gtgatgttag tgattgatgc gcgcgaaggt
    22081 atttccgatc aggatctctc gctgctgggc tttattctca atagtgggcg ctcacttgtc
    22141 attgtggtga ataagtggga tggcctgagc caggaagtga aagagcaggt gaaagaaacg
    22201 ctggacttcc gtctgggctt tatcgatttt gctcgtgtgc actttatctc tgccttgcac
    22261 ggcagtggtg ttggtaactt gtttgaatca gtacgtgaag cgtatgacag ctccacccgt
    22321 cgtgtgggga cctctatgct gacgcgcatc atgacgatgg ctgttgaaga tcaccaaccg
    22381 ccgctggtac gcggtcgtcg tgtgaagctg aaatatgcac acgccggtgg ttataatccg
    22441 ccgattgtgg tgattcacgg taatcaggtg aaagacctgc ctgattccta caagcgttac
    22501 ttgatgaact acttccgcaa atcgctggac gtaatgggat cgccgattcg tattcagttc
    22561 aaagaagggg aaaacccgta tgcgaacaag cgtaacaccc tgacgccaac ccagatgcgt
    22621 aaacgtaagc gtctgatgaa gcacatcaag aaaaataaat aattatccgt aagtatttta
    22681 tctgccattc cctctatgaa tgtagagggg tggctaccgg aggaattcat atggaactgc
    22741 attgtccaca atgtcagcat gtgcttgatc aggataacgg ccatgcgcat tgcccatcct
    22801 gcggggaagt tattgaaatg aaagctcttt gcccggactg ccatcaaccg ttgcaggtgt
    22861 taaaagcctg cggtgcggta gattacttct gtcagcacgg gcatggcttg atttcgaaaa
    22921 aacgcgttga attcgtgctg gcttaatgaa cctttttacg cgattttttt accggctgga
    22981 tgttttttac ttcactttct atccagccgt cttccagacg tgtggttagc atttcacccg
    23041 ctttaacttg cttcactttt ttcagtacat tgccgtcagt agcagtagta acgctgtatc
    23101 cacgcgccag cgttgacagt gggcttacgg cttcgaggtg cgttactgca ttaccgaaac
    23161 gttcacgcgt ggcgctaagc tgtgcgcgca gggtttctgc taaacgatat tccagttgct
    23221 gaatgcgcgt ttgcgcgcga tgaatcttcg gctgtggatt ttgctgattc agccgctgtg
    23281 ttaatcgctg ctgctgttgc ccggcacgct taagttgatt ttccagcgca aagctcatcc
    23341 gtttttgcag gcgttcaagc atggtttgct ggcgtgccag ccggagctgc ggatgctgtt
    23401 gctgtaatcg gtgatgaatt tgcgtaaagc gacgtgtgcg gttggcgaga taataatcca
    23461 tcgccatctc cagccgttga cgggtcgatt gcacctggcg cagtaactct tgctgattac
    23521 ggctcaccac ttcggcggca gccgacggtg ttggtgcacg cagatcggca acaaaatcgg
    23581 caatggtcac atccgtctca tgcccgacgg cgctgacgac cggaatgcgg ctggcaaaaa
    23641 tcgcccgcgc tacgcgttcg tcgttaaaac tccataaatc ttccagcgaa ccgccgccgc
    23701 gcccaacgat caacacgtcg cactcattgc gctgattcgc cagttcaatg gcgcgaacaa
    23761 tttgccccgg cgcgtcatcg ccctgaacgg cggtggggta gatgatcacc ggcagagaag
    23821 gatcgcgacg ttttaacaca tgcaaaatat catgtagcgc agcaccggtt tttgaggtga
    23881 tcacaccaac gcaatgcgca ggggagggaa gtggtttttt gtattgcaga tcgaacaatc
    23941 cttcagcctg caacttcgct ttgagctgtt cgtacttctg ttgcagcagc ccttcaccgg
    24001 ccggctgcat actctcaacg attatctggt agtcgccgcg cggctcgtag agtgtaatat
    24061 tggcgcgaac taaaacttgt tgcccatgct gtgggcggaa ggtcacccgg cggttgctgt
    24121 tgcggaacat cgcgcagcgt acctgggcgg tgtcgtcttt gagtgtaaag taccagtgac
    24181 cggaagccgg ttgtgtgaaa ttagagattt cgccgctgat ccaaacctgt cccatctcat
    24241 gctcaagcag cagacgaacc gtttgattca ggcgactaac ggtaaaaatt gcaggggatt
    24301 gagaaggtaa catgtgagcg agatcaaatt ctaaatcagc aggttattca gtcgatagta
    24361 acccgccctt cggggatagc aagcattttt tgcaaaaagg ggtagatgca atcggttacg
    24421 ctctgtataa tgccgcggca atatttatta accactctgg tcgagatatt gcccatgcta
    24481 cgtatcgcta aagaagctct gacgtttgac gacgttctcc tcgttcctgc tcattctacc
    24541 gttctgccga atactgctga cctcagcacc cagctgacga aaactattcg tctgaatatc
    24601 cctatgcttt ccgcagcaat ggataccgta acggaagcgc gcctggctat tgctctggct
    24661 caggaaggcg gtatcggctt tatccacaaa aacatgtcca ttgaacgcca ggcagaagaa
    24721 gttcgccgtg tgaaaaaaca cgaatctggc gtggtgactg atccgcagac tgttctgcca
    24781 accacgacgc tgcgcgaagt gaaagaactg accgagcgta acggttttgc gggctatccg
    24841 gtcgttaccg aagaaaacga actggtgggt attatcaccg gtcgtgacgt gcgttttgtt
    24901 accgacctga accagccggt tagcgtttac atgacgccga aagagcgtct ggtcaccgtg
    24961 cgtgaaggtg aagcccgtga agtggtgctg gcaaaaatgc acgaaaaacg cgttgaaaaa
    25021 gcgctggtgg ttgatgacga attccacctg atcggcatga tcaccgtgaa agatttccag
    25081 aaagcggaac gtaaaccgaa cgcctgtaaa gacgagcaag gccgtctgcg tgttggtgct
    25141 gcagttggcg caggtgcggg taacgaagag cgtgttgacg cgctggttgc cgctggcgtt
    25201 gacgttctgc tgatcgactc ctcccacggt cactcagaag gtgtactgca acgtatccgt
    25261 gaaacccgtg ctaaatatcc ggatctgcaa atcatcggcg gcaacgtggc aacagctgca
    25321 ggtgcacgcg ctttggcaga agctggttgc agcgcggtta aagtcggtat cggcccaggt
    25381 tctatctgta cgactcgtat cgttactggc gtaggtgttc cgcagatcac tgccgttgct
    25441 gacgcagttg aagccctgga aggcaccggt attccggtta tcgcagatgg tggtattcgc
    25501 ttctccggcg acatcgccaa agctatcgcc gctggcgcaa gcgcggtgat ggtaggttcc
    25561 atgctggcgg gtactgaaga atctccgggt gaaatcgaac tctaccaggg ccgttcttac
    25621 aaatcttacc gtggtatggg ttccctgggc gcgatgtcca aaggttcctc tgaccgttat
    25681 ttccagagcg ataacgctgc cgacaaactg gtgccggaag gtatcgaagg tcgcgtagct
    25741 tataaaggtc gcctgaaaga gatcattcac cagcagatgg gcggcctgcg ctcctgtatg
    25801 ggtctgaccg gctgtggtac tatcgacgaa ctgcgtacta aagcggagtt tgtacgtatc
    25861 agcggtgcgg gcattcagga aagccacgtt cacgacgtga ccattactaa agagtccccg
    25921 aactaccgtc tgggctcctg attctcttcg cccgacttca tgtcgggcga tttatattat
    25981 ctgtttcact tgcctcggaa taagcgtcaa tgacggaaaa cattcataag catcgcatcc
    26041 tcattctgga cttcggttct cagtacactc aactggttgc gcgccgcgtg cgtgagctgg
    26101 gtgtttactg cgaactgtgg gcgtgggatg tgacagaagc acaaattcgt gacttcaatc
    26161 caagcggcat tattctttcc ggcggcccgg aaagtactac tgaagaaaac agtccgcgtg
    26221 cgccgcagta tgtctttgaa gcaggcgtac cggtattcgg cgtttgctat ggcatgcaga
    26281 ccatggcaat gcagttgggc ggtcacgttg aagcctctaa cgaacgtgaa tttggctacg
    26341 cgcaggttga agtcgtaaac gacagcgcac tggttcgcgg tatcgaagat gcgctgaccg
    26401 cagacggtaa accgctgctc gatgtctgga tgagccacgg cgataaagtt accgctatcc
    26461 cgtccgactt catcaccgta gccagcaccg aaagctgccc gtttgccatt atggctaacg
    26521 aagaaaaacg cttctatggc gtacagttcc acccggaagt gactcacacc cgccagggta
    26581 tgcgcatgct ggagcgtttt gtgcgtgata tctgccagtg tgaagccctg tggacgccag
    26641 cgaaaattat cgacgatgct gtagctcgca tccgcgagca ggtaggcgac gataaagtca
    26701 tcctcggcct ctctggtggt gtggattcct ccgtaaccgc aatgctgctg caccgcgcta
    26761 tcggtaaaaa cctgacttgc gtattcgtcg acaacggcct gctgcgtctc aacgaagcag
    26821 agcaggttct ggatatgttt ggcgatcact ttggtctgaa cattgttcac gtaccggcag
    26881 aagatcgctt cctgtcagcg ctggctggcg aaaacgatcc ggaagcaaaa cgtaaaatca
    26941 tcggtcgcgt tttcgttgaa gtattcgatg aagaagcgct gaaactggag gacgtgaagt
    27001 ggctggcgca gggcaccatc taccctgacg ttatcgaatc tgcggcgtct gcaaccggta
    27061 aagcacacgt catcaaatct caccacaacg tgggcggcct gccgaaagag atgaagatgg
    27121 gcctggttga accgctgaaa gagctgttca aagacgaagt gcgtaagatt ggtctggagc
    27181 tgggtctacc gtacgacatg ctgtaccgtc acccgttccc gggaccaggc cttggcgttc
    27241 gtgttctggg tgaagtgaag aaagagtact gtgacctgct gcgccgtgct gacgccatct
    27301 tcattgaaga actgcgtaaa gcggacctgt acgacaaagt cagccaggcg ttcaccgtct
    27361 tcctgccagt acgttccgtt ggcgtaatgg gcgatggtcg taagtatgac tgggttgttt
    27421 ctctgcgtgc ggtcgaaacc atcgacttta tgaccgctca ctgggcgcat ctgccgtacg
    27481 atttcctcgg tcgcgtttcc aaccgcatta tcaatgaagt gaacggtatt tcccgcgtgg
    27541 tgtatgacat cagcggcaag ccgccagcta ccattgagtg ggaatgattt gaccctgcac
    27601 tatgaatgaa caaaaccctc tgttactaca gagggttttt tatcttcaag aatcataggc
    27661 ttgaagttag taacatcgat taattaaacc agctgtccga tttgttctct tctgctttgc
    27721 ccacgctttt catcagatcg cgaccgcctt cagtcatatt tctgttggcg tcagcttcag
    27781 attgcaccac atcggtttgc gcagctttgt gcttcagttc ctgatcgata aattcgtttt
    27841 cgcgcttaac gcgggcttct tctttcgcca gcgccagttt ttgtttctga atctctaagc
    27901 tgcgtagctc atcttcataa ctttgatcgc gttttttgtc cgcagtggct tcggcgtcca
    27961 gtttatcctg acgagctttc ttatttgccg ctgccgttgc cgctcttttg ttagcggcgg
    28021 cctgggcatt tgcgcgacgt tgcttctctt gctggatttc cctgttgcgc tccgcgaccc
    28081 attcgtcatg ctgcctttgc tcttcatttt taccttgctg ttccgcttct gcgacagcag
    28141 agagttgatc ctgcaatgat gaggcgatag ccggatagct taaggagacc aagatggcgc
    28201 aaagaaaaac tttcttcatg actcctcctg attattagct cttttcagga catttggtat
    28261 ttggctgaat acgcgtttcg ttatacgtcg tggtaataac aacggctaaa cctgtcgtaa
    28321 actggcactc tttacccacc tgagtagagg tatacacttt ggtgccttcc ttatacgtta
    28381 aagaaacacc ttccactaag gttttatcat tcaccataga acccgctgcc gcgcctacag
    28441 ctccgccgcc aactgcacct gccgtcgttc cggaattgct gccagacccg acgttgtggc
    28501 cgataacacc gccagcgact gcgccaataa gcgcgccgaa ggcttgtgcg ttccgtttat
    28561 tttgggagtt gtctacggca acttttgcgg gaagaatgga aataatatta acggttttag
    28621 tttcttgttt ggtattcagt tgatcggttt gataaacatc ggcggcatga tcgtcagcat
    28681 ttgactggca tcctgccaga gtgaatgacg ctaacattgc cacaggcaga agacattttt
    28741 taaatttcat cactattcct tgttatacac attacgaaaa ataagacatt taagatcatc
    28801 aaaagaggaa agtaaatgta ttgcgtataa agggatagat taaatttatt aaaattcact
    28861 tggggaataa tttatgaaaa cacatcatta caacatatat attaattttg cagccaaaca
    28921 ggataaacat cagtcaggac aaagacgtaa aatttaagat gaaagaatat ctgccgcttt
    28981 gataattcca aagagcaaaa gcatccgaca gcgaagcgcc gttatgttct gtgaaagtgg
    29041 agaaggtaac cgctaacgtt gtaccggtga ttcgacggag agtgtggatt gctgagcttc
    29101 ttttttgctc tgatggtgct gccaggcacc gacggacgag tagataaaac ggccaaagaa
    29161 gaagataaag ctgatgagca gtacgatacg ggtcatgcga ctgttaaatc ggtgtcgttt
    29221 tcgcatactg gttgcctgac tcacaaaagg ttccttgaag tatgtcccac gctgtggacg
    29281 gtacttacat taaggcacaa caggacaaaa tggtcaattc ttcgttatgt aaaaaagcgt
    29341 cagtggatac atattttaat gttatggaag ttaatttaat tatttacaat gatgatgtaa
    29401 taatgatgaa ttaacatgat aaacgtaata attcattaat cagggttatt ttgtttgata
    29461 tatatcaatt ggaactttca tatgacactt agaatcaatg ctctctatag tgatgaataa
    29521 tcatcattcg aagtcaggtg ggatgcctgt ctgaatacac ctccttcagg atgtggggga
    29581 ttctgctgag catctatgaa actgaatgca acttatataa aaatacgtga taaatggtgg
    29641 gggcttccgc tgttcctgcc ttctttaatc ttgcccattt tcgcccacat taatactttc
    29701 gcgcatattt cctccggtga ggtttttctc ttttatctgc ctttggcact gatgatcagc
    29761 atgatgatgt ttttcagctg ggcggcattg ccagggatcg ccttagggat ttttgtccgc
    29821 aaatatgcag agcttggttt ttacgaaacg ctctcattaa cggctaattt tattatcatt
    29881 atcattctct gttggggcgg ttacagggtc tttactcccc ggcgtaacaa cgtttcacat
    29941 ggtgataccc gtttaatttc ccagcgtata ttctggcaga ttgtgtttcc tgcaacgctg
    30001 tttctgatac ttttccagtt tgctgcattt gtaggattac tggcgagcag agaaaatctg
    30061 gttggcgtca tgccttttaa tctcgggacc ttaatcaatt atcaggcctt actggtgggc
    30121 aatctgattg gtgtcccgct gtgttacttc atcattcggg tagtgcgaaa tccgttttat
    30181 ttacgtagtt attattcgca attaaaacag caggttgatg tcaaagtcac caaaaaagag
    30241 ttcgcactct ggctactggc attaggtgct ttactgttgc tgttatgcat gccgttaaat
    30301 gaaaaaagca caatttttag caccaattat accttgtcat tattgctgcc cctgatgatg
    30361 tggggagcga tgcgctatgg ttataagctg atttcactgc tctgggcggt cgtgttgatg
    30421 atcagcatcc acagctatca aaattacatt cccatttatc ccggctatac cacgcagctg
    30481 accataacct cctccagtta tctggtattc tcttttattg tcaattatat ggctgtactg
    30541 gcaacccgtc agagaacggt agtcagacgc attcagcggc ttgcgtatgt ggacccggtg
    30601 gttcatctgc caaatgttcg cgccctgaat cgcgcgttgc gtgatgcccc ctggtctgcg
    30661 ctttgttatt tacgcatccc tggcatggaa atgctggtta aaaactatgg catcatgctg
    30721 cggattcaat acaagcaaaa actttctcac tggctgtcac cattgctgga accgggtgaa
    30781 gatgtttatc agctttcggg taacgatctc gcgctgcgac tgaatacaga atcgcaccag
    30841 gagcgcatta ccgcactgga tagccatctc aagcaatttc gtttcttttg ggatggcatg
    30901 ccgatgcaac cgcagattgg cgtcagttac tgctatgtgc gctcgccagt gaatcatatc
    30961 tacctgctgc tgggagagct aaatacggtc gccgaacttt ccatcgtgac caacgccccg
    31021 gaaaatatgc agcgtcgcgg ggcaatgtat ttgcaacgcg aattgaaaga taaagtcgcg
    31081 atgatgaatc gactacagca ggcgctggaa cacaaccatt ttttcctgat ggcccagccg
    31141 attaccggta tgcgtggtga tgtttaccat gaaattcttc tgcgcatgaa aggtgagaat
    31201 gatgaactga tcagccccga tagcttcttg ccggtcgcgc acgaatttgg tttatcgtcg
    31261 agtatcgaca tgtgggtcat tgagcatacg ctgcaattta tggctgaaaa cagagcgaag
    31321 atgcccgctc accgttttgc tattaatctg tctccaacct cggtatgtca ggctcgtttt
    31381 cctgttgaag tcagtcagtt gctggctaaa tatcagattg aagcgtggca acttattttt
    31441 gaagtcaccg aaagtaatgc tctgaccaat gttaagcagg cgcaaatcac cttgcagcat
    31501 cttcaggaat taggctgcca gattgcgatt gatgatttcg gcaccggcta cgccagctat
    31561 gcgcggctta aaaatgtgaa tgccgatctg cttaaaattg acggcagttt tatccgcaat
    31621 attgtgtcaa atagtctgga ttatcagata gtggcatcga tttgccacct ggcgcgaatg
    31681 aagaaaatgc gggtagtggc agagtacgtt gaaaacgaag agatccgcga ggcagtgctc
    31741 tctttgggga tcgattatat gcagggttat cttattggta agccgcaacc gttaattgat
    31801 acgctgaatg aaatcgaacc cattcgcgaa agtgcctgaa taatgcgggc cgacatttct
    31861 cgtcggcccg caaagcatta agcggcgatt tcaggtgtac tttcttcttc aattttcaac
    31921 cgccagccag ccacgccttc ccagtattct tgctcctttt ccagatcaag cagtaccaac
    31981 gcattctgac taaaccagtc atgcgggaaa cgcagtgtcc agtgactgtc atcagtaatc
    32041 agtgtcaatg ttggcggtgt ggtggttgcc tgacgttgat tgttgagtaa tacgccaagg
    32101 cgcaatagct gaatcagtgg caggaactgt ttcttcttaa acaaggtaaa gcgcggcaga
    32161 tcgtcgagtt taatcgcttt acggtgatag cgcaccagtg tcgccatcat cagctgctgt
    32221 tcctgattaa aacccggcaa gtcgctgttt tgtagaatat aagcggagtg acgatgcaaa
    32281 ccgctgtggt tgatattcaa cccgacctca tgcagcatgg cggcccatcg cagtagcgcc
    32341 tccagttgcg gatgcgccag cttcggttgc tgttcccgcc actgttcgta catttgcata
    32401 gtggtatcca gcacccgtcg ggcctgttcg ctgtcgatgt gatactggtt ggcgaggctg
    32461 ctggcggtgc gactacgcac atcctgatga cggaaacgtc cttccatttc atacagtacg
    32521 ccttcgcgta acgccccgtc agaaaggcgc agttcacgga tggctaaagc atcaaacaca
    32581 ccgcataaaa tcgccagtcc cggaacgaag actgttttcc gctcttcgga aagacctggt
    32641 aaactcagcg atgcgaaatt acggtgacgt aaaacttctt ttaccagttt ttccagacgt
    32701 tccggggtaa ttatcccgtc tttctcgccc atttccatta atacttcatg ggcggctttt
    32761 atggtaccgg aagcgcccat tgccacgttc cagccctgaa tacggaattg ccaggttaaa
    32821 gtttccagtt tttgtgctgc cgccatgcga gcacgctgaa aattctcttt attgatgact
    32881 ccgccaggga aataaagctg ggcaaagctg acacacccca tccggcggct ttcaacgaga
    32941 atcggttcga aattttcacc aatcaccagt tctgtagaac cgccgccaat atcaataacc
    33001 agtttgcgac ctttttccgg ttgggtatgt tccacgccca taaaaatcag acgggcttct
    33061 tcattaccgg aaataatttc aatcgggtag ggaatgacct tttccgcacg tttcagaaag
    33121 tcagtggcgt tcagcgcctg acgcagcgta tgggtaccaa ctatacagac gctggcagga
    33181 gaaaaccctt gtagccgttc ggcaaacagc gacagacagt tcaaaccgcg cgtcattgcc
    33241 tcttcactca acatattatc tggccccagg ccgtccgcca gatgcacccg ctgtttcagg
    33301 cggccaataa tctgcatggc accatctacc acacgggcta tgaccatgtg aaaactgttt
    33361 gaaccaagat cgactgcagc aaactcctgc ggacgagggg atttatcgtg tattggcata
    33421 gggttattca ggttgttcga gtgatttgat gtagtcataa atcgccaact gcgcccgtac
    33481 tttgcggcga ttgccgcggg gaacgtagcg attactgagt tctttatcga tataacgtgc
    33541 tttgaccgta tcgctgaaca atatgtcgat gatgtccagc acccgctgct tcaggcgcgg
    33601 atcgagcagc ggcgtcgcca cttcaatacg ataatcaata ttgcgcgtca tccagtcggc
    33661 ggaagaaagg tagacctttt tatcgccgcc attttcaaaa atataaaccc ggtcatgttc
    33721 aaggtaacgg tcaacaatac tgatggcacg aatgttgtcg ctaatgcctt ccagattggg
    33781 gatcagcgaa cacattccgc gaaccagcag attaaccggt acgccggagc tggaggccgc
    33841 atacagacga tcaaccaggc ctttatcgac aaggttattt agcttcaggg tgataccact
    33901 gggcagccct tgctgcgcgt tggcgatctc gcggtccacc atttcataca acaggcggcg
    33961 ggagttttgc ggcgacacca ttaaataatc aaatgtcacc ggacggtatg ggttttcaat
    34021 aaagttgaac acccgccgta cttcgttagt gatgcgcgca tcggcggtca gcaacgaata
    34081 gtcagtataa agacgcgcgg ttttttcgtt aaagtttccg gtcccaatgt gtgcgtaacg
    34141 caccacttca ccgttttctt tacgtgaaat caggaacagt ttggcgtgaa ttttcagccc
    34201 cggcgcagag aagataacgt gcacgcctgc ttcggtcagg cgcttcgccc agtgaatgtt
    34261 tgcttcttcg tcgaaacgcg cctgtaactc aaccaccacg gtgactttct taccgttgtg
    34321 tgcggcgtgg atcatcgagt cgatgatgcg tgaatctttc gccacgcggt aaatgttaat
    34381 tttaatcgcc agtacgctcg ggtcgaacga agcctgacgc agcagttcca gcacatgctc
    34441 aaaggtgtga taaggataat agagcaacac atcgcgttcg cgaatggcat caaaaccatt
    34501 gcggaactgg gctttatcaa accaaatgtg gcgtaaacgc ggcagtggtt tgttcaccag
    34561 attggctttg ccgacattgg ggaaattaat aaagtcttta aaattatgat aacgaccgcc
    34621 ggggacgatg gagtcgtagc gggaaatagt cagtttttcg cgtaacactt caaccagcgc
    34681 attgggcata tcgcgctgat aaacaaaacg caccggctca gcagttaaac gctgcttgag
    34741 actggaagac atcaactcca tcaggctggc ttccatctca tgcactaaat cgtattcggc
    34801 atcgcgggtc atcttcattg aataggcatt cagcgcgtca taatcaaaga agcctttgaa
    34861 aatatcatca aggcagtaac gcagaatgtt atccagaaga atcatcggct tgcgtcgacg
    34921 cggcgcttct ggcggtaaat tcacaaagcg cggcacttta tctgatggga tctccagcag
    34981 cgcgtaacgg atggtatcgc cacggataat ttccaccgcc agataggtgt aatcatcttt
    35041 caggaactgc actaagtcag tgtcaggatt gattaaaatc ggcgtaatgt gctgacgcag
    35101 atactgctta aaataatgac gcagccagtt ttgttgattg acggagagct ggcgttcatt
    35161 aatcaggaag atctggttgc gcgccatctc cagcaatagc tcgttgtaga ggccgtcgaa
    35221 ttcctgatcg gctttcagca cccgggactg aattttgccc agtaaatggc gggaatgaga
    35281 gttggagcct tgttcttcgc taataatgat gcgtcgcttc agttcagcga agcggacttt
    35341 atagaactca tcaaggttat tggaatagat ccccaggaaa cgcatccttt caatcagcgg
    35401 gttagatttg tccgccgctt cctgaagcac gcgttcattg aacgataacc aactgagctc
    35461 tttttcgatg tatagctttt cctgacccat taccactttt actccgtttt attcacggga
    35521 cactgcttgg atattatggc gagcaatatt ggcagatgtc caactatgcc agaaaagggg
    35581 gaaaaaaact gctggagctt gcgatttaca ggtgaattga aaaacgccag cggcagagct
    35641 ggcgctttaa ctacggggca ttactcgtcg gcagcgtagc cctgcggcgg cagacgttga
    35701 ccatccagcc acgcggcgtt ttcgtgcatt ttcagacgac catcggcaaa ccagctaatc
    35761 accagtggat aaatggcgtg ttcctgggtt tgcacgcggg cggtgacgtc atcttccgta
    35821 tcaccagcaa ataccgggac tttcgcctgt aaaataaccg ggccaccgtc cagttcatcg
    35881 gtgacgaaat gcaccgatgt accgtgctct tcatcgccat tttccagcgc ctgacgatgg
    35941 gtgtgtaatc cgggatattt cggcagcaga gaagggtgaa tgttcagcaa acgcccggca
    36001 tagtgggaga caaacgccgg gctgagaatg cgcataaaac cagccagcac gaccacatcg
    36061 ggtgcgtaca tgtcgatttc atgaatcaac tcccggtcat aggcttcacg actgtcaaac
    36121 gcgctggcga tgagcgtatg cgttgcaata cccgcctggc gggcgcgttc aaggccgaac
    36181 gcgtcggcct tattgctgaa aactgcccgt acggtgcctt taattttgtt ggttttacag
    36241 gcgtcaataa ttgcctgtaa attacttccg ttgccggaaa taagcaccac aatattcatt
    36301 attcgataac cacgcgttgt tcggaatcag aggctttgat gataccgatt ttccacgcgt
    36361 tttcaccgtt ggcattgagc agggcgaggg ctttgtccac ttccggagca ggcagggcga
    36421 taatcatccc gacgccgcag ttgaaggtgc gatacatttc atggcgctca acgttaccag
    36481 ccgtttgcag ccagttgaac acttccggcc actgccagga ggattcatca atcactgcct
    36541 gggtattatc tggcaatacg cgcggaatgt tttcccagaa gccgccgccg gtcaggtgcg
    36601 caatggcatg cacatcgacc ttttcaatca actccagcac tgacttcacg taaatgcggg
    36661 tcggtgccag cagatgatcg gctaatggct taccatcaag ttcggtggtt tgcggatcac
    36721 aaccgctgac ttcaagaatt ttgcgcacca gcgaataacc gttcgagtgc ggaccgctgg
    36781 aaccgagtgc aatcagcaca tcgccgtcgc tgactttaga gccgtcgatg atttctgatt
    36841 tttctaccac gcccacgcag aaacccgcga catcgtaatc ctcaccgtga tacatccccg
    36901 gcatttctgc cgtttcgcca cccaccagtg aacagcccga ttgcagacaa ccttccgcaa
    36961 tgccgctgat caccgctgaa gcggtatcaa catccagttt tccggttgcg taatagtcga
    37021 ggaaaaacag cggttctgca ccttgcacca ccaggtcatt aacgcacatg gcgaccagat
    37081 caataccaat ggtgtcgtga cgttttaagt ccattgccag acgcagcttg gtacctacgc
    37141 cgtcagtgcc ggaaaccagc acgggttcac gatatttttg cggcaatgca cacagcgcac
    37201 cgaagccgcc cagaccgccc atcacttccg gacgacgcgt tttcttcact acgcctttga
    37261 ttcttccaac cagagcatta cccgcgtcaa tatcaacacc ggcatctttg tagctaagag
    37321 aggttttatc ggtcactgct tgggtcccca cgcgttactt gcggtagaaa aataaaattc
    37381 ggcgcaattc taacagggaa agcaaacgtt tgcgagactg ctttacacaa cctttttgca
    37441 cgtcttttcc ccaggcgcgc ggcgaaagaa gacttgtgcc agggcaaagg ttagttttcg
    37501 gatggaataa tcttctttca taaccatttg aatataaaat aactttatct caaaccgtta
    37561 tcattttgac taaagtcaac gaaaataata ttgccgcctt gaagaaagga ggtataatcc
    37621 gtcgattttt tttgtggctg cccctcaaag gagaaagagt atgaagatcg tggaagtcaa
    37681 acacccactc gtcaaacaca agctgggact gatgcgtgag caagatatca gcaccaagcg
    37741 ctttcgcgaa ctcgcttccg aagtgggtag cctgctgact tacgaagcga ccgccgacct
    37801 cgaaacggaa aaagtaacta tcgaaggctg gaacggcccg gtagaaatcg accagatcaa
    37861 aggtaagaaa attaccgttg tgccaattct gcgtgcgggt ctggggatga tggacggtgt
    37921 gctggaaaac gttccgagcg cgcgcatcag cgttgtcggt atgtaccgta atgaagaaac
    37981 gctggagccg gtaccgtact tccagaaact ggtttctaac atcgatgagc gtatggcgct
    38041 gatcgttgac ccaatgctgg caaccggtgg ttccgttatc gcgaccatcg acctgctgaa
    38101 aaaagcaggc tgcagcagca tcaaagttct ggtactggta gctgcgccag aaggtatcgc
    38161 tgcgctggaa aaagcgcacc cggacgtcga actgtatacc gcatcgattg accagggact
    38221 gaacgagcac ggatacatta ttccgggcct cggcgatgcc ggtgataaaa tctttggtac
    38281 gaaataaaga ataaaaataa ttaaagccga ctttaagagt cggctttttt ttgagtaaag
    38341 cgcctataac acataataca gaggataata ctatgacgcg ccgtgctatc ggggtgagtg
    38401 aaagaccgcc acttttacag acaatcccgc ttagtttgca acatttgttc gccatgtttg
    38461 gtgcaaccgt cctggtgccc gtcttatttc atattaaccc ggcgactgta ctgttattta
    38521 acggtattgg aacgctgctg tatctcttca tctgtaaagg gaaaattccg gcttatcttg
    38581 gttccagctt tgcctttatt tcaccggtat tgttactgtt gccgttaggg tatgaagtcg
    38641 cgctgggcgg ctttattatg tgcggcgtgc tgttctgcct ggtttctttt atcgtgaaga
    38701 aagcggggac cggctggctg gacgtgctgt ttccacctgc ggcaatgggc gcaatcgttg
    38761 ccgtcatcgg tctggagctg gcgggcgtag ctgccggtat ggcgggttta ctcccggctg
    38821 aagggcaaac gccagactcc aaaaccatca tcatctctat taccaccctg gcggtcacgg
    38881 ttttaggttc cgtgctgttt cgtggtttcc tggcaattat cccgatttta attggcgtgc
    38941 tggtggggta cgcgctctct ttcgcaatgg gaattgtcga taccacgccg attattaatg
    39001 ctcactggtt tgcgctgcca accctctata cgccgcgctt cgagtggttt gccattctga
    39061 ctattctgcc agcggcgtta gtggttattg ccgaacacgt agggcacctg gtagtaacgg
    39121 ctaatatcgt caaaaaagat ctgctgcgcg atccaggtct gcaccgttcg atgtttgcta
    39181 acggcctgtc gaccgtgatc tccggcttct ttggctctac gccaaatacc acttacggag
    39241 aaaacattgg cgtgatggcg atcacccgtg tttacagtac ctgggttatc ggcggggcgg
    39301 cgattttcgc tatcctgctt tcctgcgtcg gtaaactggc tgctgctatc cagatgatcc
    39361 cattgccggt gatgggtggt gtttcgctgc tgctttacgg tgtcatcggt gcttccggta
    39421 ttcgtgtgtt gatcgaatcg aaagtggatt acaacaaagc acagaacctg atcctgactt
    39481 ccgtgatttt gatcatcggc gtcagtggcg cgaaggtaaa cattggcgcg gcggaactga
    39541 aaggtatggc gctggcgacc atcgtcggta tcggcttaag tttgattttc aaactgatta
    39601 gtgtgctgcg ccctgaagaa gtggtgctgg acgcagaaga cgcggatata acagacaaat
    39661 aatgtaccga ccgggcagtg ttgctgcccg gttcaaacat catgggattc tgtggtaaac
    39721 ttctcgcgat tttgtgaaat cctggttgag gtatctctga acacaccggc acagctctct
    39781 ttgccacttt atcttcctga cgacgaaacc tttgcaagtt tctggccggg ggataactcc
    39841 tctttactgg ccgcgctgca aaacgtgctg cgtcaggaac atagcggtta catctatctc
    39901 tgggcacgcg aaggcgcggg gcgcagccat ctgctgcacg cggcttgcgc ggaattgtcg
    39961 cagcgtggcg atgcggtggg ctatgtcccg ctggataaac gcacctggtt tgttccggaa
    40021 gtgctcgacg gtatggagca tttgtcgctg gtctgtatcg acaacattga gtgtattgca
    40081 ggcgatgagt tgtgggagat ggcgattttc gatctctaca atcgaattct ggaatcgggc
    40141 aaaacacggt tgttgatcac cggcgatcgt ccaccgcggc agttgaatct gggattaccg
    40201 gatctcgcgt cgcgactcga ctgggggcag atctacaaat tgcagccact ttctgatgaa
    40261 gataagttgc aggcgctaca gttacgcgcg cgtttgcgtg gttttgaact gccggaagat
    40321 gtggggcgtt tcttgctgaa gcggctcgac agagaaatgc gcacgctatt tatgacgttg
    40381 gatcagttgg atcgtgcgtc gattaccgcg caacgtaagc tgaccattcc gtttgtgaaa
    40441 gaaattctga agttgtagat aaggtgttta ttgtcggatg cggcgcgaac gccttatccg
    40501 aactacgcga agttaatcca tagtattcgt aggccggata agacgtggca agcgtcgcat
    40561 ccggcaatgt gcttcaacta gcgatggagc acatcttaac ccacgatttc cagtacctgc
    40621 tccggtggac gaccaattcg cgctttgcca ttcgcgacca caatcgggcg ctccatcagc
    40681 ttcggattat ccaccattgc ctgaatcagc gcctcttcgc taagcgaact gtcagccagg
    40741 ttcagttctt tataaagatc ctctttctgg cgcatcaatt cacgggcgct attcatcccc
    40801 agcattttca gcaaatcgcg cagcgttgcc gcatcggcgg gtgtctcaag gtagagcacc
    40861 acttctggct ccacgccgtt ttctttcagc aggttcagcg tttcccggct ctttgaacag
    40921 cgtgggttat ggtagatttt tacctgtttg gtcattgttc ttcctttaat gcgaattaca
    40981 tcttggtata aggcttaaag cgttcctgca gctggcgcaa ctggtcgatg cgcgcatcgt
    41041 aacgcgcttg ttgcaggctg cctaatttca cctgcgaact ggcgctactc aacagcgaaa
    41101 tggcctgatc gagtcgtccg gcgagcgcat aaccttctgc tcgcgcagcc agctcctgat
    41161 cgcggttatt tagcgcggct tccgcctgtg ccagcaaatc ccagccgttg ctgtcatctt
    41221 tattattaaa ggtgtagcga ttcagaatat tggccgcttc ttgtggttga ccgccttgca
    41281 gataagcgtt cgccaggttg agctgcaaca caggattggt gcgtaaatcg cgggcatttt
    41341 tcagacgatt gatcgcctca ttggctttgt tttgcccaag atcgatatca gtagccagat
    41401 cgagatacca tgcgttgcca ggttctgccg ccagtaacgg ttgcagcgtt ttacgcgcct
    41461 cgtcgtattt attggcttcc attgcctgta aagcacgacc atattgtgct gctcgttgct
    41521 gacgaacgtt tcctttcgcc cattcatcca gcaaatcact ggtgagctgg ttacgtccgg
    41581 aattatacat ccccagtgtg cgcgctttcg ccagatagaa atcttccgac gactgcacca
    41641 ccatcgggcg catctgatta gcacggttgc gggcatctgc cagacgactt tccggcaacg
    41701 ggtgagtcaa taaaatttcc ggcggacgcg aggagtaacg cgcctgatcg agtaattttt
    41761 cgaggaaggt cggcatcgcc tgcggatcga atcctgagcg ttgcagcacc tgaataccaa
    41821 tgcggtccgc ttcctgttca ttttgctggg tgaaactgat catcccctga cgcgttcccg
    41881 ccagtgtacc ggtcagcgcc gccatccccg cctgcggact ggccatcgcc agtaaaatag
    41941 aacctaacgc gccgacccag gtcagcggcg cgctgcgctg ctgatcttcc atcgctcgcg
    42001 ccaggtgacg ttgggtgacg tgggagattt cgtgcgccat aactgaagcc agttgacttt
    42061 cgttatcgga ataacggaac agggcagagt gcagcaccac gttgccgcca aagaaagcaa
    42121 aggcgttaat ttcgtcgttg ttgatcagaa aaaaatgaaa cggtgtctta accgaattgg
    42181 catgcgaaac cagacgcatc cccagcgaat taatatattg cgttaacagc gggtcattaa
    42241 ttaacggcgc gctgccgcgt agctggcgga cataatagtc gcccatctgc atttcctgac
    42301 caatggaaag cgtgcttcct gcggaggttc ccatatccgg caaggtgtct gcgctgtcgg
    42361 caaacgccgg ggctacctga ccaatggtca tagcagcaat gagggttgca accaggtttt
    42421 ttttcaactg cctgaacata acctctatcc tgtatttctg aagatagtca tttgaccgat
    42481 actgtgaaat atcgttccca ccgcgccgcc tttgagtgta acggcgaaga ttagcccact
    42541 caaatgactt ttcagtttcc tgaatccgtc ataaaaagcg aagtcttttt tgtgacattt
    42601 cgatacaatt gcggatcgca atcccgctct tgaggttcag ggaaggtctt tatgctcgaa
    42661 atgttgatgc aatggtatcg ccgccgtttt agcgacccgg aagcgattgc cttgctggtt
    42721 attttagttg ccggatttgg cattatcttt ttctttagtg gcctgcttgc tccgttgctg
    42781 gtggctattg tgctggccta tttgctggaa tggccaaccg tgcgcctgca atctattggc
    42841 tgctcccgcc gctgggcgac gtcgattgta ttggtggttt ttgtcggtat attgctactg
    42901 atggcgttcg tggtactgcc tatcgcctgg caacagggca tctacttaat ccgcgatatg
    42961 ccggggatgc tcaataagct ttctgacttt gccgccacgt tgccgcgccg ctatccggcg
    43021 ttaatggatg cgggcattat tgatgcaatg gccgaaaata tgcgcagtcg gatgctgacc
    43081 atgggcgatt cggtggtgaa aatttccctc gcctcgctgg tcggtttgct gaccatagcc
    43141 gtctatctgg tgctggtgcc attgatggtc ttcttcctgc tgaaagacaa agagcagatg
    43201 ctgaacgccg ttcgtcgggt gctgccgcgt aaccgtgggc tggcaggaca ggtgtggaag
    43261 gagatgaatc aacaaatcac caactatatc cgcggcaaag tgctggagat gatcgtggtg
    43321 gggatcgcca cctggctggg gttcttgctc ttcgggctga actattcgct gctgctggca
    43381 gtgctggtcg gcttctcggt tcttattccg tacattggcg catttgtggt gaccattccg
    43441 gtgataggcg tggcgctatt ccagtttggt gctggcacgg aattctggag ctgcttcgcg
    43501 gtgtatctga ttattcaggc gcttgacggc aacctgttag taccggtgtt gttctccgaa
    43561 gcggttaacc tgcatccgct ggtgattatt ttatcggtgg tgatcttcgg tggtttgtgg
    43621 ggattctggg gcgtattctt cgccattcca ttggcgacgc tgatcaaagc cgtgatccac
    43681 gcctggcccg atgggcaaat agcgcaagaa taatcttcag cccgacgaac tgtttcgccg
    43741 ggctgatttt tcaggattcc tgacgtaaat aaatagcccg ataacacata ctcaccagca
    43801 ctgcaccgcc gataatatta cccagcatca ccgggagcag attggcggta ataaaatggc
    43861 ttaccgtcag tgccggaaaa ttgtctgcgc tactgtgcgc cagttgccag aaggaggtag
    43921 gggcgaaatg gcgaatggca atggcgaacg gaatcacaaa caaattggcg atacagtgct
    43981 caaagccact ggcgacaaac agggtgatgg gcaaaatcat ggcgacgatt ttgtcgcata
    44041 acgaacgccc gcaataactc atccacagcg ccaggcaaac catcagattg cacataatgc
    44101 cgaggctgac agattcagta aatgtatgat gcattttgcc ctcggcgcag tgtaaaaccg
    44161 cgactcccca catcgcgttt tcactcatca ccagcccgga aaaccagatt aacaaactga
    44221 aacaggcaat acctgccaga ttaccgcagg ccaccagaag tgcgttaatc agccaagttc
    44281 gccaactaat aacgccccga ctttttgcca tcaccgtcat taccgacgag gtgaacaggc
    44341 tggtgccgca aaccgccagc aaaataaagc cgagtgtaaa gcaaaggccg cccaccagat
    44401 gggttaatgc ctgtgaagac ggggcatcgg cgataacgga aaggtaaaaa gtaaatccga
    44461 tcgccataaa cacgccagcg gacatcgcca ttaaaaagac cgacacttta ctgcgggcaa
    44521 ttttatgggc ggcaatccgt tcagcgattg ccgcttttct ggcgctcaac tgcaagtcga
    44581 aagagagttt gtttctcatg catactcctg caaaagcaga ttacaacacc tcgcgaaccg
    44641 agatccccag acgctgcatt cgtgacagca gcgtggtgcg cttcatccct aatcgtgtcg
    44701 ccgcgccacg gggcccggca acaatgccat tggtttctcg caatacctga acaatgcgct
    44761 gacgttcttc ttcgtcattc tctggcgtcg tcgggtgcag caactgcgcc attgaactgc
    44821 gaagcgctga atcttcattt agcgtcggca gtaaacggct ttgtcggaca ttaagatgta
    44881 aattcagact gttaccacga gtcaacagta ccgcccgctc aatcacgttt tccagctcgc
    44941 gcacgttgcc cggccaatcc cacgacatca actggcgtag cgcctcggtc gggatggcgt
    45001 caattgcgcg attcatatgg cgcgccattt tttgcgtgaa atgttttgct aaaagaggga
    45061 tatcttccgg acggtcgcgt agcggcggca attccagtgg gaagacattc aggcgataaa
    45121 agagatcgct gcgaaactgg cgatcttcaa ccatttgcca caaatcacgg ttggtggcgg
    45181 caatgactct gacattcacc gggatcgttc tactcccgcc gagacgctca atctcccgct
    45241 cctgcaatac gcgcagcagt ttaggctgaa gttctaacgg cagatcgcca atttcatcga
    45301 gaaacaacgt gccgccatcg gcaatttcaa aacgaccacg atgggtatta atcgcaccag
    45361 taaacgcccc tttgtcatga ccgaataact cactttccag aagactggcg gggatggcag
    45421 cgcagttgat tttgaccagc ggcttgtcgc gtcgcgggct aagttgatgg atcgctctgg
    45481 cgattacctc tttgccagtt ccggtttcac cgcaaatcaa caccgtactg tcgctcttcg
    45541 ccacaatatc tacctgctgg agcaggtctt ccattgcctg gctttgatag ataatgtcac
    45601 cgacgcccag attcgaaagg agctgctcgc taagctggtg gttttcttgc tgcaaacttt
    45661 cctgcaaatc ggtcatgcta cgccaggcat cggcattgcc aacggcaata gcgatgcgat
    45721 cggctatgtg ttgtagtagc tggcagtttt cctcactaaa gagagtggaa gaggtatgcg
    45781 ccagcaacaa tgcacccggt gtatggttgc caaaggtaag cggtataagg agcgcagatt
    45841 cgcagccgtt agatgcaagt aagtgtaata acgggtcgcg ttgccagaga aacagatcat
    45901 ctgcacggtg cgtcaaggtc ggttgtcggg tttgtagcgt ttgtatcagc aatacactgt
    45961 cgccaggcat attgcgtggc agacacgcac aatgtgaggc agaaagatcg ctgcaccaca
    46021 ggctgaactt ctcgttcttt cgatgatcgc ccagtaccat actgactgaa gccagaccga
    46081 aaaaatgatg gatctcacgg gcgacgtcag cgatcagatc gtcgagatca agatgtgaca
    46141 gcactgcatt agtaatatca accagtaccc gatgatgatc gcaatctttc ttcagcaaac
    46201 gcagcgccac ctgctgttct tcatcattga gccatgattg cagaaccggg gtgataatgc
    46261 gtgccagcca gttaaacagt gtgcaatcct ggggagtaaa acgactgccg tgggtgcgaa
    46321 taaagcgtag ctccgccatt ccactactgc cgagaaggac ggcgtgacgc gaataatcga
    46381 ccatctcgcc ctgatactct actgttgccg tcacatcgct gccatcatca tggtagcggt
    46441 aatagaagtt gcttttgaga acatgcagtt caataagttg taccgggctg aacggcggcg
    46501 gcagcgggat gaaggcgcgt aataaagacg ccttgccgtg tttctgcgct aaccgctccg
    46561 cgagcattcc gtttaccgct tcaattgtta ttccttgcgg cggggcaaac atcgcctcgt
    46621 ctgacatagc catcaacagt ccttattaat gagaaatcct tctcacgcaa tttttctggc
    46681 catcagatag atggccggtt cctgcacaat ttcatccaac agatgaatga attgaatgct
    46741 acgcgcctgc cacacagacg tcgcgtcatc cgccagtggc gcgaatgccc gggtcaacag
    46801 ggcggtgtgg ctggtgtcaa taacaatctc accaaaagtc atcaccccca gcatcttacg
    46861 tcgggcttgc tcctcttcga ccagtgcaag ccaatcttcg tattccgtca gtgggcagca
    46921 gaaggcgaca ttcagacaat caatcacgcc aacgtgatga ccgatagcca gtgagtaata
    46981 cattacctga gagctgtgtt ccggcatctc gtcgctactg gcgacaaact tttttcgcag
    47041 cgtccagaaa acaatttccc cgcactcctc agccatgtaa cccccggaga acgcgaaaga
    47101 gttgctgaac gatactattg agacgcggat cgtcggcatc gcgcaaccag gtgttaaccc
    47161 gatttcctgt aggatcatcg cttaaatgac gcaggagccg atcgcaaatt tctcgcccct
    47221 gacgatagcc cgtcagccgc cgcgcttctc gctcaatggc gatacgctgt gatggcggga
    47281 tctgcggcca caacggttgc atagtcaccc cggtgggatc gcgataatcc acagcgtgaa
    47341 tcttctgttg cagcaaaccg agcgccacgg cgaaaccgtg aatggtggcg gccggtgttg
    47401 gcgggcagcc ggggatccaa acatcaatgg ggacaatggt gtcgctgccg ccccagacgc
    47461 tgtagagatc gtggaaaata ccgccgccga caccgcacgc gccgtaggaa acacaaattt
    47521 tattatcggg ggcagactca tacgcccgaa gtgcaggcat acgcatcgcc cgggtgactg
    47581 cgccagtaaa taacaaaata tcggcgtgac gcggtgatga aacaacctta atgccaaaac
    47641 gttctgcgtc gaatacgggt gtgatggcag caaaaatttc gatttcacag gcgttgcagc
    47701 cgccgcaatc gacgcgataa acgtaagccg agcgacggat atcttgtaac aaatgctgct
    47761 tcatcttttg cgtttgctcg tccagcgtga tgggctggct gacatgttgc gtaagcactg
    47821 gactcatagc tgctccttag ccaccagcgg tacatctgta tcgtcgttga tcagcgtcgc
    47881 gcgttgtttg cactccgggc agacgctcgc ttgcgcccgc aacatttcgc ggttttgtgg
    47941 tgcattttgt tgctgtgcca acaattcagc agccagtgcg acggtttttt gcggggcaaa
    48001 cgggcgttcg caacggctgc aacgttgtag atggaacgtc gcgcgggtat agagatcggc
    48061 tttattggtg acggtcagtt caaagttatt ggtaagctgg atggctctgg tcgggcacac
    48121 ttcttcacaa cgtccgcagt aaatacaacg ccccagatag agctgccagg tgcgcgaatt
    48181 ttgctggtcg tcggtctgga tagtcagcgc atttgccgga caagcacagg cgcaggcacc
    48241 gcaggcaata cattggctgg gcatcaggtc cggtttcccg cgaaagcctg ggctgacctc
    48301 cagtggcgcg aagggatatt tcaccgtcgc ggttccggcg cgcataatag ttttcagtaa
    48361 cttcagcatc ttctgtcctt acttcagcgg cgaacggtta cgatcaatgc cgtagcgttc
    48421 gatctcttta tacggcacgg tttttgactg gcgcttgcgc acatcaacca gcgtcacacg
    48481 gtcggtacag gagtagcagg gatcaaggct accgataatc agcggtgcgt cagaaacggt
    48541 attgccgcgt agcatgtaac gcagcaccgg ccagttggcg taggtggcgg cacgacagcg
    48601 ccagcggaac aatttttggt tatcaccgag catgctccag tgcacgtctt caccgcgtgg
    48661 cgcttcaaca aagccgagcg cgaatgcgtg aggtttatag ctaaagcctt cggtcagcag
    48721 cggggtatcc ggcatgttgt cgagggcaaa ttccagcatt gccagcgaat caaacgtctc
    48781 tttgacacgg accatcacgc gggagaaaac atcgccgccg gtgaaggtaa acagtgtttt
    48841 tggaatattg ccgtagtcgg cgtaggggtg atcaaagcgc aaatcacggg caaaaccact
    48901 gccgcggatc agcggcccta cagggctata atcacgggcg atttgtcggt cgagaatgcc
    48961 aatgccctga gtgcgttgtt ccatattcgg cgtagcaagc agcatctcta ccagctccga
    49021 cacgtcggcg cgcatctcgc gcaccagttt cagcgtttgc agacgttgct ctttgagaat
    49081 atcgcggcga acaccaccaa tcagattcag accgtaggtt ttacgcgacc cggtcagcaa
    49141 ttccgccatt gtcatcgact tttcccgcac gcgaaaaaat tgcataaagc cggtatcaaa
    49201 accaacaaaa tggcaggaga ggccaaggtt gagcaaatga ctgtgtagcc gttcgacttc
    49261 cagcagaatc gagcgaatgg tatgcgctcg ttgcggcacc tcaatcctca gtgcattttc
    49321 aaccgagttg gtataggcca cactgtgggc aaaaccgcaa atcccacaca cgcggtccga
    49381 taagaaggtc acttcgttgt agcccatccg cgtttctgcc agtttctcca tgccgcgatg
    49441 gacataaaac agacggtaat cagcatcgac aatttgctcg ccatccacaa acaagcggaa
    49501 gtgacccggt tcatcggagg tgatatgcag cgggccaata gggatcaccc gcgcgtcgct
    49561 gtcgccctcg ttgataaacg gatacgtttc ggtatcagtc gtcggttcag ggcgcagtcg
    49621 ataatccatc gcgtctttgc gcagcggatg catatcttcc ggccagtcat cgggcaacac
    49681 caggcgacgc tgatccggca ggccaaccgg aatcagcccg tacatatcgc gaatttctcg
    49741 ctcgccccag accgcggcag ggacgcgcgg tgtgactgac ggaaactccc gactgtcggc
    49801 atcgaccagt gccttcacca cgatccagca tttttcggcc ccttccatag aaagggcata
    49861 ataaacggcg taatgaccgt taagtgtccg ctcgtcgttg ccaaacagga ccggaagcca
    49921 gccatcatgt tgataataaa gatactgtac aacgtcaggc agcagattga ttttcaccgt
    49981 aatggtgacc tgttcaagcg tttgtcgctc ttcatccagc accgcgccgg ggaactgcgt
    50041 tttcagggcg gcgagaatcg cttcgccacg atttgacgat gaattaacgt tcactgtgtt
    50101 tctccgaaac agatgcggtg cctgaaggca aaaaatcatg ccaggtgcta cgttgtgcag
    50161 gcaggtcgtg cgtccctgag aggactatag tggaagcgcc tgccaggatc ctgatgacag
    50221 gttgtggaat atgcgttccc atcgccagca tcatgaccag cagaatcacc attggcgagg
    50281 tggtcaacca gccaagttca ccccggttaa cggcctgcgg cggtttcgcc attaacaccc
    50341 gcgcagccat ccgtaccagg cccgccagca ccagcgttaa cagcaataac agcaggacga
    50401 taatcagcag atgattacgt gccagtccgg cggtaacggt cataaattcg ctaagaaaaa
    50461 tgttgaaggg cggcatccct gccagcgcca gcgcaccgcc gccaaacagc acggcggtaa
    50521 atggcatgat tttgagcatc ccacagacga cgttgagatc gcgcgtgccg tacttgagca
    50581 gtacattgcc ggaaccgcag aacagcagcg tttttgccag actgtggttt aaggtgtgca
    50641 gcagcgcggc aaaaattccc agcggcccgc caatgcctag cgccaccgcg accagtccca
    50701 tgttctccac gctggagtac gccagcagac gcttaatgtc ccgctgtacc agaatgaaaa
    50761 atgccgccac ggcaaccgac aacatgccga agatgagcaa caaccggttg gggaaatcgc
    50821 tgccgatggc ctggcaaata atgatgtaat agcgaatcag caccaacagc gcgcagttca
    50881 gcaatacggc ggagagtagg gcgctgaccg gactcggcgc ttcactgtga gcatccggca
    50941 gccaggcgtg catgggaaat agcccggttt tggtaccaaa gccaattagc acaaacacaa
    51001 aggccaacag cattaatgtt ggatcaagca aggacgattg cttaagaacc tcgctccaga
    51061 atatcgccat ttctgcctgc ggcataacgc tggcggcgtt ggcgtatacc agcacggtac
    51121 cgaacagacc aaaagcgaca ccaacagtac aaataatgat gtacttccat gcagcttcca
    51181 gcgatgaacg ctgaccgtaa atgcctacca gaaacgccga gcttaaggtg gtggcttcga
    51241 tcgccgccca catcacaatc aggttattgc tggtaacaac cagcagcatg gtgaacaaaa
    51301 acagatggaa gaaaccgtag taatcgcaca gcgtaacggg tgaaagctcg ccgtgctcca
    51361 cttcatgacg catgtagcca atcgagtaaa caccggtaag aaagccaatc acaccaagaa
    51421 tggcgaggaa caaaccgccc agaccatcaa tatgaagcca cagtcccgca gcgaatattt
    51481 ctcccgcatc agcggcggtt tggaccaccc agagtgccag aatcagcagt agtgtgatcc
    51541 ctaagctatg taatacggtc accgcgcgag tcgcagagtg cccccgtttc cggcaggcaa
    51601 aacagagcag cgaaaaaagc agcggcgtga gcaggagtaa agcgaacatc acagaataac
    51661 tcatctcatt atcccttcag cgcggtcaag ttgttcacgt ccagcgtgcc gtgggtacgc
    51721 catatttttc ttgccagtaa caccatcaca atgacggcga agatggcgtc ggtagcgata
    51781 cctatttcca ccagttccgg tgcacgccag gcaagaagcg ccagcaccag atgggagccg
    51841 ttttccatca ggcagtaacc aaaaatttgc cgcaggatat tgcgctggct gacaatgcac
    51901 agcaggccaa gcagaaaatg acctaacgct accgccagcg ccggtttcag cccggtagcc
    51961 atcggtagct tcacgggctg aacgacaaat gcgcaaagca ggacaattaa cgccgcgagc
    52021 agtgccatca ttgccggacc gaataacgct ttttccggga tgttctgggg aatatttcgt
    52081 gcagcgtaag tcatgattaa cggtaccagc agcactttgg tgataaaggc gctggcggac
    52141 cagatcagca gttgctctgc ggcgaacagg cacgagagag tggcgaaaat agacaccagc
    52201 accagcgact ggcaggcgta aaatccgcat gacaggcgat agcttttgac gctaatcaca
    52261 aacagcgatg tcagcatcat cagtcccgcc agattattta cgatcataga accggtcatt
    52321 catcatctcc ttactgcaac caggtcgcgg cgattacgct ggaaatcagc gacatcacaa
    52381 tcagtacaat caacaccagg cgcattgatc ctggcagcgg tgcggcatcg gcgacggcct
    52441 cgctcggttt gccaggcacg acgcgaccaa accagcgaat aaaccaagcg aaactggcga
    52501 ccgattcaat catcagcaga atcatggcgg gcagcaggat ccagtactcc actgacaacg
    52561 caaaaccggc ggcaaacagc gggaatttac tgaagaagcc gttgaacggc ggcacgccgg
    52621 taatcgccag cgctgccacg cagaaaccca cgcctggcaa cggcagggtg tgcaatacgc
    52681 cacgcagacg cggcaacaag cgcgtgccgc agctgtaact cagcgcacct gctacaagga
    52741 aaaacaggct tttagcgaac gcgtggttga cgatgtaggc gatgctaccc tccagcgcca
    52801 gccgcgagcc gaagatggag agcgacaagc cgaagaacat ccagccaagt tgagtgatgg
    52861 tcgaccaggc cagcaaccgc ttcatatcct gctgtggcaa atacatcaga aatccataaa
    52921 gaatggtgac cagggccatg accatgccaa caccgccaat cacatgcggg atattgccgc
    52981 cgtcgataat ggcgcgggca aaaatgtaaa cgcccacttt caccatcgat gcggcgtgga
    53041 gataggcgct gatcggtgtt ggcgcttcca ttgcgtccgg tagccacgct tgcatcggta
    53101 gctgggccga tttcccccac gcggcaaaca ggatgccgcc ataaaccaga taacgtgcgt
    53161 cgccgtgtaa ctcgctcatc gcgctaagcg caaacgttcc ggtctgcaaa aacagcgtgg
    53221 cggcggcaag atacaacccc aacgagccga tatgggtgat aagcaacgct tttagtgctg
    53281 aacgctgcgc tttatcgctc tggtaataac tgatcaacgc ccaggagcag ccgcccgtaa
    53341 tttcaaaaaa caacaactga ccgagcagcg tcgaggagag taccagtccc gccatcgcgc
    53401 cgataaacaa cagcaaaaat gcgtaataac gattcgtgcc gttatgcggg tgttcgcgat
    53461 ttttatccgt cagataaccc gtggagtaga tcgtgaccag caaaccgaga aacaccacca
    53521 caaacagaat cagcgtactc acgcgatcaa tgaccaggcc aaacaatgcc acatccccca
    53581 cgttgaccaa cgtcaacgtg acggcaactt tatcggcctg ataaaacgcg gagatcagtg
    53641 acaacatgca cagcgtggtc agcgcggcga acaaaacccc ccattcggcg gcccgacgtt
    53701 gtggcgaaaa cgaaacgacc agtgcgccga taaaaggcag caataacgtc gtcagagcaa
    53761 gattttccat attccgtcag tgctccttac agaccggtta accagaagac ccatgcaagc
    53821 gcagcaaggc tgaagccaag ccaggtcaca tggtgaatga gtaaaaaacg cccgcgtgcc
    53881 agcgtgtttt ctgcgataga ggccagaaca aaaatcagca aaaccttgag cagcgtaacg
    53941 acaagtgaag tcagcaggca ggcgagagaa agctcttgcg cgcgcccaaa gggcagaaac
    54001 agggccacaa acagtgctgc catcacgacc tgtttcagcc ccagccccca tttcgctagc
    54061 gccagcccgg caccggaata ttcggtcagc gggccttcct gtaattcctg ttctgcttca
    54121 gcaacatcaa agggaatttt tcccatctca atgaagcagg caaaaccaca ggccagtaac
    54181 gccagtacgg tggttagcgg cgagttccag cccgttgcca gcgtattgct gatcatctcg
    54241 atatgcgtgg aacctgctat cagcgccaat accagcagtg agagaatgag catgggttcg
    54301 accagaatgc cgagcgtcaa ctcgcgactg gcaccgactc ccgcaaacgg gcttccggta
    54361 tccagcccgg aaagagcaaa gaaaaaacga aacagggcaa gaagatagat aagggtgatc
    54421 agatcgccgc cgcccgcaaa aggggaaacg gtaataaata gtggtagggc catcgccagc
    54481 accagcatgc tgctgattaa tacccacggc atcaggcgga acatcagacc tgaagatgtc
    54541 ggcgcaactt cctggcgttt aaacagtttg tggatatcgc gataatcctg ccagatcccc
    54601 ggtccgcggc gggagtgcat tcgcgcgcgt atctgccggg aaatacccgt aaaaagtggg
    54661 gttagcatca gcagaatcac ggcctgtgct aacgcaaaaa tgaccagata tccgtcgcaa
    54721 agagtttgtc tcatggtgat ttccttagac ggcaatagcg attagcagca caaccagtgc
    54781 ggcgaccaga tacaggcaat agagacgaaa gtcgccgctt tgcagatgct gtatttcttt
    54841 ggccagccgt tgggtggcgc tcacgatggg gcggatcacc cgctcatccc agaagggttc
    54901 tgtgctctga gccctggcgg tgacgtgcgc aagaccttta tttagcctca gcgtagggtc
    54961 gagctgttgt cgtagacgaa atagcgcaga aaagaccaca cgcagcggct gcatcacgcc
    55021 attgcctgac ggggccatcg cattttccca gccgtagccg catgcccagg catctcctgt
    55081 gcgacgaaac gctgcgcggc gcgaacgaca ccacagccag taaaggccag gcagcaaagg
    55141 catcgccagt agtaacagca acagtaatga gggggtgacc tgtgtatgaa acgtgccggg
    55201 tacaagtgct attccgctgg cgacagtgac gggaggggta ttggtaaacg catgggcaat
    55261 atgcattatc ttcggtgcca gccaactggc actaagcgca attaatacgc agagtgccgc
    55321 gagcagtagc atggcgacga tcatcgtatt tggcacttcc tgtgcctctt cagcgtgtgt
    55381 actgcgcggc gcaccacaga aagtaatacc gtacattttt acgaagcaca ttactgccag
    55441 cccaccagtg actgccagca ttaccatagc aataggaccc gcaagttgta gcgctacggc
    55501 ttccacacga cttagtgaga acagcgactg ccaggtgtac cattcgctga taaaaccatt
    55561 cagcggagga atggctgata tcgcgaggca accaatcagg catgctgcgg ctgtccacgg
    55621 catccgtttc gccagtgccc ccattttttc catgtcgtgg gtatgcaaac gcgaaataat
    55681 cgcgcccgcg ccgagaaata gtagcccttt gaacagcgca tggttgagca gatgaaacag
    55741 tgcgccgagc agtccaacca cggtgagcag cgggtcgtgc agtgacagac cgaccatcgc
    55801 cacaccgact gccagcaaaa taatgccgac gttttcgacg gtactccagg ccagcagccg
    55861 tttgatatcc tgttccgcca gcgcatatag cacgcccagg agcgcggaga ttgtgccgat
    55921 cgccatcacc agaatgcccc accacagcgg caaacccgtt tgcgccagca gatccatcgc
    55981 cactttcagg atgccgaaaa taccgatttt gaccattacg ccagacatca acgccgaagc
    56041 gtgcgatggt gctgcagggt gagcgcgcgg caaccagctg tgcaacggca tcatcccggc
    56101 tttcgcgcca aaaccgaaaa aggccagcag gaacaccgcc gacgccagcc ccggagaaag
    56161 tgaaagcgtg cggaaactgg caaaatcgag gctgccgctt tcgcgtccca tcagcaagaa
    56221 ggcgatcata atcagcaccg atccggcgtg ggcgataaaa aagtagagca tgccagcgtt
    56281 gatcgacgtt ttatcctgcc tggcaatgac cagaaaccag gaagacagtg acatcatttc
    56341 aaacagcacg atgaaccaaa aagcgttgtc catcaccagc agggcaacca tcgatgcgat
    56401 gaaaatattc ataaagaagc ccatcgccgc cgcgcctttg ccctcgtatt cgcgcatata
    56461 agtcaatgaa tagagcgaac aaaccaccac cagcaaggag ataaccagca ccataaatgc
    56521 cgagaggcta tccattcgca gggttaaatc ggcaaagtca aaaggggtgg caaaacgggc
    56581 gactaaagtt acgcctgcat gtaattgcgt aattccgctg ataatgccaa tcacgccgcc
    56641 caccagcgat gtgatgccgg aaagcttaat agccagtctg tccagaccga gtaaaaacag
    56701 cgaagccaga ctggcaaaca gatagaggat cagcgaccag gttaataatt gcagggcatc
    56761 cattagcgtt gctcctgagt gagggaaagg ggatcctccc cgccattggc aaaccagctt
    56821 gcggcaaggc gctgtttttc tttcatctga cgttgcaggc tatcgtcggt gatcagccgt
    56881 aacgcctgat tcgggcaagc gcgaacgcag gccggacctt gtggcaagaa atcacacagg
    56941 tcgcatttca ccgcgacggt ctggacaccc ggttcccagc gcagcaaagc atgttgggtt
    57001 ggcgcgtttt cttcgccgtc tttgagtgag ccttcagcct gaaaaacata ttgcgcaggg
    57061 gcattcaccg gacggcttcc tgaagcgctg attgcgccaa atgggcagac cacggcgcaa
    57121 agcttgcagc caatacagag gttttcgttg agttggatcg catcatccct ctgggagatg
    57181 gcattgaccg ggcagacctg caggcacggg gcttcctcac agtgatgaca cacgacaggg
    57241 gcagtgagtg ttgacgtctt cgccagggcc aggcgcgggt gttgctgtaa accttgcgtt
    57301 ttatgcacgt ccgaacaggc agcgagacag gtattacatc ctgtacacca cagtggttcg
    57361 gccaccacaa agcggttcat gtcggctcct tgaaaatgat atgaaacatt gcatttcata
    57421 ttgcataaag gatgccagtc tgattaattt aatatattca ataagttatg aattaactgg
    57481 attctgaacg gtacaaaaat gatggtcatt aacgttcagc aaaaaatgga ttcgacaccc
    57541 gtgtcgcagg ggaacgcgag aatgtgatct tgatcacgag aagtgaggta tgctgaccgc
    57601 aagcgcagcc agcacggaat ggagcaaagt aatcaggcgt gttctttcaa ccagttcagc
    57661 acaacgtcgt ggtgattgct ggttttgaaa tcgtcaaaga catgttcgat tttgccatca
    57721 gcgtcaatca ggaagctgat gcgatgaata ccatcgtagg ttttgcccat gaaggacttt
    57781 tccccccaga cgccgaattg ctcgcacacc tggtggtcct catcagacag gagcgtaaag
    57841 ttaagcagct ctttttccgc aaaacgggag agtttttcgg gtttatcggt gctgataccc
    57901 agcacatcaa cgcccgcttt tttcaactca tccatgttat cgcgtaagcc gcaggcctgt
    57961 acggtacagc cgggggtcat ggctttcggg tagaaataaa ccagaacacg ctgtccctgg
    58021 aagtcggtca aattaacttg ttctccgtct tgatccggca agctaaattt cggtgcgata
    58081 tcaccggctt tcagtggatt cattacttaa ctccatcctg ttcatcatgt tgggaataat
    58141 tgacgacgtt aatactgcct tgtgcattga gttctgtaca tagggctttg aacgcttgct
    58201 caatatttgc tgcgtccgca gatgcggggc tgtgggcagt aatctgaata tgcaactgcg
    58261 cagcccgttc attttcagca ggttgcgtgc gcgacaccag ctccgcaatg ttcatatgat
    58321 gcgcgtcgaa aagtgctgtg aagcgttcaa ttaaatgcgg ggagtctgcc acatcgacct
    58381 gaacccagac agatgctggc attggcggac gcggacgcgc cgtcgtgcgc ttcatcacga
    58441 ttaaaagatc cagttcggca cctttcaacg gtaacgttga ttcaatcaga gtaatggcat
    58501 tccatgaacc ggaaagcagc atgataaacg tgaactcttc tcccagcatc gccaggcgac
    58561 tgtcttcaat attacagccg caactactga catgacgggt gatggtgttc acaattccag
    58621 ggcgatcggc acccaacgca gtgatcacca gataatgttg cgatgacagt gtcaaactgg
    58681 ttattccttt aaggggtgag ttgttcttaa ggaaagcata aaaaaaacat gcatacaaca
    58741 atcagaacgg ttctgtctgc ttgcttttaa tgccatacca aacgtaccat tgagacactt
    58801 gtttgcacag aggatggccc atgttcacgg gaagtattgt cgcgattgtt actccgatgg
    58861 atgaaaaagg taatgtctgt cgggctagct tgaaaaaact gattgattat catgtcgcca
    58921 gcggtacttc ggcgatcgtt tctgttggca ccactggcga gtccgctacc ttaaatcatg
    58981 acgaacatgc tgatgtggtg atgatgacgc tggagctggc tgacgggcgc attccggtga
    59041 ttgccgggac cggtgctaac gctactgcgg aagccattag cctgacgcag cgcttcaatg
    59101 acagtggtat cgtcggctgc ctgacggtaa ccccttacta caatcgtccg tcgcaagaag
    59161 gtttgtatca gcatttcaaa gccatcgctg agcatactga cctgccgcaa attctgtata
    59221 atgtgccgtc ccgtactggc tgcgatctgc tcccggaaac ggtgggccgt ctggcgaaag
    59281 taaaaaatat tatcggaatc aaagaggcaa cagggaactt aacgcgtgta aaccagatca
    59341 aagagctggt ttcagatgat tttgttctgc tgagcggcga tgatgcgagc gcgctggact
    59401 tcatgcaatt aggcggtcat ggggttattt ccgttacggc taacgtcgca gcgcgtgata
    59461 tggcccagat gtgcaaactg gcagcagaag ggcattttgc cgaggcacgc gttattaatc
    59521 agcgtctgat gccattacac aacaaactat ttgtcgaacc caatccaatc ccggtgaaat
    59581 gggcatgtaa ggaactgggt cttgtggcga ccgatacgct gcgcctgcca atgacaccaa
    59641 tcaccgacag tggtcgtgag acggtcagag cggcgcttaa gcatgccggt ttgctgtaaa
    59701 gtttagggag atttgatggc ttactctgtt caaaagtcgc gcctggcaaa ggttgcgggt
    59761 gtttcgcttg ttttattact cgctgcctgt agttctgact cacgctataa gcgtcaggtc
    59821 agtggtgatg aagcctacct ggaagcggca ccgcttgcgg agcttcatgc cccggctgga
    59881 atgattttgc cggtgacctc cggtgattat gcaatcccgg tgaccaacgg tagtggtgct
    59941 gtcggtaagg cgctggacat tcgtccacca gcccagccgc tggcactagt ttctggcgcg
    60001 cgtacccagt tcacgggcga taccgcttca ttgctggtgg aaaatggtcg tggcaatact
    60061 ctgtggccgc aggtggttag cgtgctgcag gcgaaaaact acaccatcac ccaacgtgat
    60121 gatgctggtc agacactgac caccgattgg gtacaatgga accgtctgga cgaagacgag
    60181 cagtatcgtg gtcgttatca aatctctgtt aagccgcagg gttatcagca ggcggttacg
    60241 gttaaactgc tgaacctgga acaggcgggc aaaccggttg cagacgcggc ttccatgcag
    60301 cgttacagca cggagatgat gaacgttatt tccgccggtc tggataaatc tgccactgac
    60361 gccgcgaacg ctgcgcaaaa tcgtgcctcc accactatgg acgtacaaag tgcagctgat
    60421 gacaccggtt taccaatgct ggtcgtacgc gggccgttca atgtggtttg gcaacgtctg
    60481 ccagcggcgc tggaaaaagt gggcatgaaa gtgaccgaca gcacccgttc gcagggcaac
    60541 atggccgtaa cttataagcc gctgtctgac agcgactggc aggaactggg cgcgagcgat
    60601 ccaggcctgg catccggtga ctataaactg caggttggcg atttagataa ccgcagcagc
    60661 ctacagttca tcgatccgaa aggtcatact ctgactcaga gtcagaacga cgcgctggta
    60721 gctgtcttcc aggctgcgtt tagcaagtaa aaatacaggg ctggaatcat ccggcccttt
    60781 tttctgatat gatacgcaaa cgtgtgcgtc ggcaggaaaa cgcgatttta gcggtaattc
    60841 gcacgaaatt tgtttgtcgg acgtagttcg gataaggcgt tcacgccgca tccgacaaaa
    60901 catccggcac accagacagc aaaagatttt aaaacgttaa ttcacaccca ggagtgataa
    60961 agatgcaaaa gcaagctgag ttgtatcgtg gtaaagcgaa aaccgtatac agcacggaaa
    61021 acccggacct gttggtgctc gaattccgca atgatacgtc agcaggggat ggcgcgcgca
    61081 ttgagcagtt tgatcgcaaa ggtatggtga acaacaagtt caactacttc attatgagca
    61141 aactggctga agcgggtatc ccgactcaaa tggagcgtct gctctccgat accgaatgtc
    61201 tggtgaaaaa gctggatatg gtaccggttg agtgtgtcgt gcgtaaccgt gctgcgggct
    61261 ctctggtgaa acgtcttgga atcgaagaag gtattgagct gaacccgccg ctgttcgatc
    61321 tgtttctgaa aaacgacgcc atgcacgatc cgatggtcaa cgaatcttac tgcgaaacct
    61381 ttggctgggt aagcaaagag aacctggcgc gtatgaaaga gctgacctac aaagcgaacg
    61441 acgtgctgaa aaaactgttc gatgatgctg gtctgattct ggtcgacttc aagctggaat
    61501 ttggtctgta caaaggcgaa gtggtactgg gtgatgagtt ctccccggac ggtagccgcc
    61561 tgtgggacaa agaaacgctg gagaaaatgg acaaagaccg tttccgccag agcctcggtg
    61621 gcctgatcga ggcctatgaa gccgtcgccc gccgcctggg tgtacagctg gactgatttt
    61681 tctgttcatc atcttgccgt gctgctggca cggcaagaca accattctcg taagatgtgc
    61741 attgaacgct cattcctccg ccatttcatc ccgctttcct gtggtaatct tcctctgaac
    61801 cacctacgat catttctata atgaatatat tgttgaggta tctatgcgtt ggcaagggcg
    61861 acgtgaaagt gacaatgttg aagacaggcg caacagctct ggtggtccgt ctatgggcgg
    61921 tcccggtttt cgcctgccga gcggtaaagg cgggctgatt ttactgatag tcgtgctggt
    61981 tgcaggctac tatggcgttg atttaaccgg gttgatgacc ggacagccgg tttcccaaca
    62041 gcaatcaacg cggtcaatta gcccaaatga agacgaagcc gcaaaattca cctcggtgat
    62101 tctggcaact acggaagaca cctggggaca acagttcgag aagatgggta agacctatca
    62161 gcaaccgaag ctggtcatgt accgtggaat gacgcgtacc ggatgcgggg cgggccagtc
    62221 cataatgggg ccgttctatt gcccggcgga tggcacggtt tatatcgatc tctccttcta
    62281 tgatgacatg aaagacaaac ttggcgcgga tggcgatttt gcccaggggt acgttatcgc
    62341 ccatgaagtc ggtcatcatg tgcagaaact gttaggcatc gagccgaaag ttcgtcaact
    62401 gcaacaaaac gcgacgcagg cggaagtgaa ccgcttatct gtgcgtatgg aactccaggc
    62461 cgactgtttt gccggtgtct gggggcatag tatgcagcag caaggcgttc tggaaaccgg
    62521 cgatctggaa gaggcgctga acgcggcgca ggccatcggc gatgaccgtt tacaacagca
    62581 aagtcagggg cgagtagtac cagacagttt cactcatggc acttctcagc aacgctacag
    62641 ctggtttaaa cgtggtttcg acagcggcga tccggcacaa tgcaatactt ttggtaaaag
    62701 catttaactt ccggggcagg gatggctgaa ctgactgcgc ttcacacatt aacagcgcaa
    62761 atgaaacgtg aagggatccg ccgcttgctg gtgttgagcg gggaagaggg ttggtgtttt
    62821 gatcatgcgc ttaagttacg tgatgcctta cctggcgact ggctgtggat ttcgccgcag
    62881 ccagatgctg aaaaccactg ttctccctcg gcactacaaa ctttacttgg gcgcgagttc
    62941 cggcatgcgg tattcgacgc ccgctacggc tttgatgccg ctgcctttgc ggcacttagc
    63001 ggaacgttga aagcgggaag ctggctggtt ttgttactcc ctgtatggga agagtgggaa
    63061 aaccaacctg atgccgactc gctgcgctgg agtgattgcc ctgaccctat tgcgacgccg
    63121 cattttgtcc agcatttcaa acgcgtactt acggcggata acgacgctat cctctggcgg
    63181 caaaaccagc cgttctcgtt ggcgcatttt actccccgta ctgactggca ccccgcgacc
    63241 ggcgcaccac aaccagaaca acagcaactc ttacagcagt tactgaccat gccgtcgggc
    63301 gtggcagcgg taactgctgc gcgtgggcgc ggtaagtcgg cgttggcagg gcaactcatt
    63361 tctcgtattg cgggcagtgc gattgtcacc gcgcccgcaa aagcggcaac ggatgtactg
    63421 gcacaatttg cgggcgagaa gtttcgcttt attgcgccgg atgccttgtt agccagcgat
    63481 gagcaagccg actggctggt ggtcgacgaa gccgcagcca tacctgcgcc gttgttgcat
    63541 caactggtat cgcgttttcc tcgaacgttg ttaaccacta cggtgcaggg ctacgaaggc
    63601 accggacgtg gttttttgct gaaattttgc gctcgctttc cgcatttaca ccgttttgaa
    63661 ctgcaacagc cgatccgctg ggcacaggga tgcccgctgg aaaaaatggt tagtaatgca
    63721 ctggtttttg acgatgaaaa cttcacccat acaccacaag gcaatattgt catttccgca
    63781 tttgaacaga cgttatggcg aagtgaacca gaaacgccgt taaaggttta tcagttattg
    63841 tctggtgcgc actaccggac ttcgccgctg gatttacgcc gcatgatgga tgcaccaggg
    63901 caacattttt tacaggcggc tggcgaaaac gagattgccg gggcgctgtg gctggtggat
    63961 gaggggggat tatctcaaca actcagtcag gcggtatggg caggttttcg tcgcccgcgg
    64021 ggtaatctgg tggcccagtc gctggcggcg cacggcagca atccactggc ggcgacattg
    64081 cgcggacggc gggtcagccg gatagcagtt catccggctc gtcagcggga aggcacagga
    64141 cagcaactta ttgctggtgc tttgcaatat acgcaagacc tcgactatct ttcggtgagt
    64201 tttggttaca ccggggagtt atggcgtttc tggcaacgct gcggttttgt gctggtgcga
    64261 atgggtaatc atcgtgaagc cagcagcggt tgctatacgg cgatggcgct gttaccgatg
    64321 agtgatgcgg gtaaacagct ggctgaacgt gagcattacc gtttacgtcg cgatgcgcaa
    64381 gctctcgcgc agtggaatgg cgaaatgctt cctgttgatc cactaaacga tatcgtgctt
    64441 tctgacgacg actggcttga actggccggt tttgctttcg ctcatcgtcc gctattaaca
    64501 tcgttaggtt gcttattgcg tctgctacaa accagtgaac tggcattacc ggcgctgcgt
    64561 gggcgtttac agaaaaacgc cagtgacgcg cagttatgta ccacacttaa actttcaggc
    64621 cgcaagatgt tactggtccg tcagcgggaa gaggccgcgc aggcactgtt cgcacttaat
    64681 gatgttcgca ctgagcgtct gcgcgatcgc ataacgcaat ggcaattttt tcactgactc
    64741 cttcaatttt ctcccatggt cattgtgagt cagcggcgta aaattaatga catcagttaa
    64801 ggagttcgcc atgaaacatg accattttgt tgttcaaagc ccggataaac ctgcgcaaca
    64861 gttgttactg cttttccacg gtgtcgggga taacccggtg gcaatggggg aaataggcag
    64921 ctggtttgcg ccgctatttc ctgatgcgct ggtggtgagt gtcggaggcg cggagccgag
    64981 cggtaatccg gcggggcgtc agtggttttc ggtgcagggt attacggaag ataatcgcca
    65041 ggcgcgtgtg gatgcgatca tgccgacgtt tattgaaacg gtacgctact ggcagaaaca
    65101 gagtggagta ggggctaacg ccacggcgct aattggtttc tcacaaggcg cgattatggc
    65161 gctggagagc attaaagccg aaccgggcct tgcctcacgc gtcattgcct ttaacggacg
    65221 ctatgcgagc ctgccggaaa ccgcgtctac agccacgaca attcacctga ttcatggcgg
    65281 tgaagatccg gttatcgatc tggcgcatgc agttgcggcg caggaagcgt taatcagtgc
    65341 cggtggtgac gtgacgctgg atatcgtgga ggatttaggc catgcgatcg acaatcgcag
    65401 tatgcaattt gccctggatc acttgcgtta taccattccg aaacattatt tcgacgaagc
    65461 gctaagcggc ggtaagcctg gcgatgatga tgtgattgaa atgatgtaat aatagccggg
    65521 taggcctgat aagaccgtat cagacatcat ggccggatgc ggcgttaacg ccttatccgg
    65581 ccactcagct tacttcttcg gttgatcctt tttcggccag tcgtcgtcat cgtcccactt
    65641 atcgttgaaa tcacgatgcg ggggaagctc cggcttgttg gcgagaaatt tcttatggtc
    65701 aacgcgcttg agatctttaa tcacattcag caggacgcct accaaaaaaa caatcaccag
    65761 aatccaccaa tatttagcca gccagtccat gcttatttcc tcttgcagaa ccactcatca
    65821 cgcgacgagc tgttccatga tacgttgata catacgggca agtagctgca ggtcggcagc
    65881 gttcacacat tcattaattt tatgaatagt ggcattgacc ggcccgagtt ccaccacctg
    65941 cgcccccatg cgggcaataa agcgcccgtc ggacgttccg cctgtggtca gtagctgcgg
    66001 tttaatttca ttatagtgct caaccgcgtt aacgaccgca tccaccagtt taccgcgcgc
    66061 ggtcaaaaat ggctgcccgg aaagccacca atccaccgta tagcgcagtt gatgtttttc
    66121 aagcagggca agcacctgcg ctttgatcat ctcatcagtc agttcggtgc tgaagcggaa
    66181 gttaaactgc acaaacagtt cacccggaat aacgttgtta ctgcccgttc ccgcctgaat
    66241 attggcaatc tgcatactgg tcgccgggaa aaattcattg ccctgatccc actcaatagc
    66301 cactaattca ttaaggaaag gtgctgcgcg atgtaccgga ttgtcagcca gatgtgggta
    66361 ggcaacatgc ccctgaacgc catgaatggt aaggttgcag gttaacgatc cgcgacgacc
    66421 atttttcacc acatcaccta ccacttcgat actcgacggt tcgccaacca ggcagtaatc
    66481 gagacgctca ttacgtgcca ttaacgcttc gacgactttt accgtaccgt tgtgggcact
    66541 ggcttcttca tcagaggtga tcagaaatgc cagtcgcccc gtatggttgg gatgttgtgc
    66601 gacaaaacgt tctgcagcta ccaccatcgc cgccagcgag cctttcatat ctgccgcacc
    66661 gcgcccgaat aacatgccgt cacgaatggt tggttcaaac ggcggattga tccaacgatc
    66721 ggcgtcgcca ggcggcacca cgtcggtatg cccggcaaag gctaacgttt caccttgccc
    66781 acgccatgcc caaaaattct gcgtatcggc aaagtccatg cgttcaacgg taaaaccgat
    66841 cgcctgcaaa cgttcaatca acaaagcctg gcatcctgcg tcatcaggac tcagggaagg
    66901 gcggcgaata agctgttgtg tcagctcaat aaccgggcac gacatagact acacctcatg
    66961 gaaaaattgc tgataactgg aatcactgaa acccagcagc ataggcttac caggcgcgca
    67021 gagcaatgga cgtttgataa ttgcaggcat ttcagtcatt aatgccgccg cagaggccgc
    67081 atcggtgatt ttattgcggg tggtttcgtc cagtttacgc caggttgtac cacgggtgtt
    67141 gagtaacgct tcccagccta attcgttgat aaaaccgttc aataattcgc tgtccagccc
    67201 atcgacgcgg taatcatgaa aacgatagtc gatgttattg gcttccagcc aacggcgagc
    67261 ctttttaatg gtgtcacaat ttttgatgcc gtaaagtgta accataatga atcctttttc
    67321 gatttatgag aataaatact catttaaggg tgaaaacatt ttgcctgcaa atgattatca
    67381 ggaaatataa cattatcga
//
LOCUS       DAZFKF010000018        66695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFKF010000018 DAZFKF010000000
VERSION     DAZFKF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.018"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(147..1046)
                     /gene="pgrR"
                     /locus_tag="ACHKA1_001410"
     CDS             complement(147..1046)
                     /gene="pgrR"
                     /locus_tag="ACHKA1_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO5679087.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSYPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLRGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            1184..2104
                     /locus_tag="ACHKA1_001411"
     CDS             1184..2104
                     /locus_tag="ACHKA1_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5679088.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            2104..3033
                     /locus_tag="ACHKA1_001412"
     CDS             2104..3033
                     /locus_tag="ACHKA1_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5679089.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHL"
     gene            3054..3692
                     /locus_tag="ACHKA1_001413"
     CDS             3054..3692
                     /locus_tag="ACHKA1_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5679090.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            3983..4711
                     /gene="mpaA"
                     /locus_tag="ACHKA1_001414"
     CDS             3983..4711
                     /gene="mpaA"
                     /locus_tag="ACHKA1_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO5679091.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(4686..5651)
                     /gene="ycjG"
                     /locus_tag="ACHKA1_001415"
     CDS             complement(4686..5651)
                     /gene="ycjG"
                     /locus_tag="ACHKA1_001415"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO5679092.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQQILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFNLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEQALQFTTGELHL"
     gene            5770..6276
                     /gene="tpx"
                     /locus_tag="ACHKA1_001416"
     CDS             5770..6276
                     /gene="tpx"
                     /locus_tag="ACHKA1_001416"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO5679093.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(6320..7861)
                     /gene="tyrR"
                     /locus_tag="ACHKA1_001417"
     CDS             complement(6320..7861)
                     /gene="tyrR"
                     /locus_tag="ACHKA1_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO5679094.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(8009..9070)
                     /gene="ycjF"
                     /locus_tag="ACHKA1_001418"
     CDS             complement(8009..9070)
                     /gene="ycjF"
                     /locus_tag="ACHKA1_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO5679095.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQVQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(9067..10464)
                     /gene="ycjX"
                     /locus_tag="ACHKA1_001419"
     CDS             complement(9067..10464)
                     /gene="ycjX"
                     /locus_tag="ACHKA1_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO5679096.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            10619..11617
                     /gene="ycjW"
                     /locus_tag="ACHKA1_001420"
     CDS             10619..11617
                     /gene="ycjW"
                     /locus_tag="ACHKA1_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5679097.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPHQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMSLK"
     gene            complement(11668..12573)
                     /gene="ompG"
                     /locus_tag="ACHKA1_001421"
     CDS             complement(11668..12573)
                     /gene="ompG"
                     /locus_tag="ACHKA1_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO5679098.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(12618..13700)
                     /locus_tag="ACHKA1_001422"
     CDS             complement(12618..13700)
                     /locus_tag="ACHKA1_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5679099.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKERNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(13714..14373)
                     /gene="pgmB"
                     /locus_tag="ACHKA1_001423"
     CDS             complement(13714..14373)
                     /gene="pgmB"
                     /locus_tag="ACHKA1_001423"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO5679100.1"
                     /translation="MKLQGVVFDLDGVITDTAHLHFKAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGDKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLCAQQIPIGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDFLTWSRLSAFWQN
                     V"
     gene            complement(14370..16637)
                     /gene="ycjT"
                     /locus_tag="ACHKA1_001424"
     CDS             complement(14370..16637)
                     /gene="ycjT"
                     /locus_tag="ACHKA1_001424"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO5679101.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEIFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLCLLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGLSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHREDE"
     gene            complement(16634..17689)
                     /gene="ycjS"
                     /locus_tag="ACHKA1_001425"
     CDS             complement(16634..17689)
                     /gene="ycjS"
                     /locus_tag="ACHKA1_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5679102.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVNPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQARVMRDTARRQGKVLAYDFHHRFALDTQQLREQVTNGILGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(17699..18487)
                     /gene="ycjR"
                     /locus_tag="ACHKA1_001426"
     CDS             complement(17699..18487)
                     /gene="ycjR"
                     /locus_tag="ACHKA1_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO5679103.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEVLAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(18505..19557)
                     /gene="ycjQ"
                     /locus_tag="ACHKA1_001427"
     CDS             complement(18505..19557)
                     /gene="ycjQ"
                     /locus_tag="ACHKA1_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO5679104.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPAGAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(19588..20430)
                     /gene="ycjP"
                     /locus_tag="ACHKA1_001428"
     CDS             complement(19588..20430)
                     /gene="ycjP"
                     /locus_tag="ACHKA1_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO5679105.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(20417..21298)
                     /gene="ycjO"
                     /locus_tag="ACHKA1_001429"
     CDS             complement(20417..21298)
                     /gene="ycjO"
                     /locus_tag="ACHKA1_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO5679106.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(21319..22611)
                     /gene="ycjN"
                     /locus_tag="ACHKA1_001430"
     CDS             complement(21319..22611)
                     /gene="ycjN"
                     /locus_tag="ACHKA1_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5679107.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLSTTLQASQKKLDELIEQH"
     gene            complement(22625..24304)
                     /gene="ycjM"
                     /locus_tag="ACHKA1_001431"
     CDS             complement(22625..24304)
                     /gene="ycjM"
                     /locus_tag="ACHKA1_001431"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFO5679108.1"
                     /translation="MKQEITDYLAEIYGGTFTSTHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSGKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSEEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSN
                     AEGNCLTGLFNVSKNIQHVNITGLHGRDLISEVDILGNKITLRPWQVMWIK"
     gene            complement(24514..24828)
                     /gene="pspE"
                     /locus_tag="ACHKA1_001432"
     CDS             complement(24514..24828)
                     /gene="pspE"
                     /locus_tag="ACHKA1_001432"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO5679109.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(24903..25124)
                     /gene="pspD"
                     /locus_tag="ACHKA1_001433"
     CDS             complement(24903..25124)
                     /gene="pspD"
                     /locus_tag="ACHKA1_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO5679110.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(25133..25492)
                     /gene="pspC"
                     /locus_tag="ACHKA1_001434"
     CDS             complement(25133..25492)
                     /gene="pspC"
                     /locus_tag="ACHKA1_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO5679111.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(25492..25716)
                     /gene="pspB"
                     /locus_tag="ACHKA1_001435"
     CDS             complement(25492..25716)
                     /gene="pspB"
                     /locus_tag="ACHKA1_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO5679112.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(25770..26438)
                     /gene="pspA"
                     /locus_tag="ACHKA1_001436"
     CDS             complement(25770..26438)
                     /gene="pspA"
                     /locus_tag="ACHKA1_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO5679113.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLVRMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            26605..27582
                     /gene="pspF"
                     /locus_tag="ACHKA1_001437"
     CDS             26605..27582
                     /gene="pspF"
                     /locus_tag="ACHKA1_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO5679114.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIILDPFKRRPPEEANAVSENTSLPMLP
                     LDLREFQMQQEKELLQHSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(27702..28967)
                     /gene="puuE"
                     /locus_tag="ACHKA1_001438"
     CDS             complement(27702..28967)
                     /gene="puuE"
                     /locus_tag="ACHKA1_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO5679115.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHDVSTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(29005..30285)
                     /gene="puuB"
                     /locus_tag="ACHKA1_001439"
     CDS             complement(29005..30285)
                     /gene="puuB"
                     /locus_tag="ACHKA1_001439"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO5679116.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTTKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(30287..31774)
                     /gene="puuC"
                     /locus_tag="ACHKA1_001440"
     CDS             complement(30287..31774)
                     /gene="puuC"
                     /locus_tag="ACHKA1_001440"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO5679117.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFPGSSRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAGLAAAIGPTIFVDLDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(31924..32481)
                     /gene="puuR"
                     /locus_tag="ACHKA1_001441"
     CDS             complement(31924..32481)
                     /gene="puuR"
                     /locus_tag="ACHKA1_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO5679118.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(32508..33272)
                     /gene="puuD"
                     /locus_tag="ACHKA1_001442"
     CDS             complement(32508..33272)
                     /gene="puuD"
                     /locus_tag="ACHKA1_001442"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO5679119.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            33484..34902
                     /gene="puuA"
                     /locus_tag="ACHKA1_001443"
     CDS             33484..34902
                     /gene="puuA"
                     /locus_tag="ACHKA1_001443"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO5679120.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQDGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            35051..35215
                     /locus_tag="ACHKA1_001444"
     CDS             35051..35215
                     /locus_tag="ACHKA1_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679121.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            35205..36590
                     /gene="puuP"
                     /locus_tag="ACHKA1_001445"
     CDS             35205..36590
                     /gene="puuP"
                     /locus_tag="ACHKA1_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO5679122.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            36724..36969
                     /gene="ymjA"
                     /locus_tag="ACHKA1_001446"
     CDS             36724..36969
                     /gene="ymjA"
                     /locus_tag="ACHKA1_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFO5679123.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            37282..38925
                     /gene="sapA"
                     /locus_tag="ACHKA1_001447"
     CDS             37282..38925
                     /gene="sapA"
                     /locus_tag="ACHKA1_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO5679124.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            38922..39887
                     /gene="sapB"
                     /locus_tag="ACHKA1_001448"
     CDS             38922..39887
                     /gene="sapB"
                     /locus_tag="ACHKA1_001448"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO5679125.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            39874..40764
                     /gene="sapC"
                     /locus_tag="ACHKA1_001449"
     CDS             39874..40764
                     /gene="sapC"
                     /locus_tag="ACHKA1_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO5679126.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            40764..41756
                     /gene="sapD"
                     /locus_tag="ACHKA1_001450"
     CDS             40764..41756
                     /gene="sapD"
                     /locus_tag="ACHKA1_001450"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO5679127.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            41758..42564
                     /gene="sapF"
                     /locus_tag="ACHKA1_001451"
     CDS             41758..42564
                     /gene="sapF"
                     /locus_tag="ACHKA1_001451"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO5679128.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(42615..43778)
                     /locus_tag="ACHKA1_001452"
     CDS             complement(42615..43778)
                     /locus_tag="ACHKA1_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFO5679129.1"
                     /translation="MARVSPSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(43787..45160)
                     /locus_tag="ACHKA1_001453"
     CDS             complement(43787..45160)
                     /locus_tag="ACHKA1_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFO5679130.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(45164..48271)
                     /locus_tag="ACHKA1_001454"
     CDS             complement(45164..48271)
                     /locus_tag="ACHKA1_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFO5679131.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYLVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     SRRQE"
     gene            complement(48271..49392)
                     /locus_tag="ACHKA1_001455"
     CDS             complement(48271..49392)
                     /locus_tag="ACHKA1_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO5679132.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VTELTGRTSAAFSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(49541..50107)
                     /locus_tag="ACHKA1_001456"
     CDS             complement(49541..50107)
                     /locus_tag="ACHKA1_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO5679133.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            50585..51373
                     /gene="fabI"
                     /locus_tag="ACHKA1_001457"
     CDS             50585..51373
                     /gene="fabI"
                     /locus_tag="ACHKA1_001457"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO5679134.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSNIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            51517..52644
                     /gene="yciW"
                     /locus_tag="ACHKA1_001458"
     CDS             51517..52644
                     /gene="yciW"
                     /locus_tag="ACHKA1_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO5679135.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLERLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISTLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            52712..54646
                     /gene="rnb"
                     /locus_tag="ACHKA1_001459"
     CDS             52712..54646
                     /gene="rnb"
                     /locus_tag="ACHKA1_001459"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO5679136.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(54765..54827)
                     /locus_tag="ACHKA1_001460"
                     /pseudo
     CDS             complement(54765..54827)
                     /locus_tag="ACHKA1_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            54881..56866
                     /gene="pdeR"
                     /locus_tag="ACHKA1_001461"
     CDS             54881..56866
                     /gene="pdeR"
                     /locus_tag="ACHKA1_001461"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO5679137.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLNEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(56848..56976)
                     /gene="ymiD"
                     /locus_tag="ACHKA1_001462"
     CDS             complement(56848..56976)
                     /gene="ymiD"
                     /locus_tag="ACHKA1_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO5679138.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            57014..57187
                     /gene="yciZ"
                     /locus_tag="ACHKA1_001463"
     CDS             57014..57187
                     /gene="yciZ"
                     /locus_tag="ACHKA1_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO5679139.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            57277..58026
                     /gene="yciT"
                     /locus_tag="ACHKA1_001464"
     CDS             57277..58026
                     /gene="yciT"
                     /locus_tag="ACHKA1_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO5679140.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            58295..58513
                     /gene="osmB"
                     /locus_tag="ACHKA1_001465"
     CDS             58295..58513
                     /gene="osmB"
                     /locus_tag="ACHKA1_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO5679141.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(58639..58965)
                     /gene="yciH"
                     /locus_tag="ACHKA1_001466"
     CDS             complement(58639..58965)
                     /gene="yciH"
                     /locus_tag="ACHKA1_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO5679142.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAPPVRPKGDGVVRIQRQTSGCKG
                     KGVCLITGIDIDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(58965..59702)
                     /gene="pyrF"
                     /locus_tag="ACHKA1_001467"
     CDS             complement(58965..59702)
                     /gene="pyrF"
                     /locus_tag="ACHKA1_001467"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO5679143.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPYGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(59895..61064)
                     /gene="lapB"
                     /locus_tag="ACHKA1_001468"
     CDS             complement(59895..61064)
                     /gene="lapB"
                     /locus_tag="ACHKA1_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO5679144.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(61071..61379)
                     /gene="lapA"
                     /locus_tag="ACHKA1_001469"
     CDS             complement(61071..61379)
                     /gene="lapA"
                     /locus_tag="ACHKA1_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO5679145.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(61528..62292)
                     /gene="pgpB"
                     /locus_tag="ACHKA1_001470"
     CDS             complement(61528..62292)
                     /gene="pgpB"
                     /locus_tag="ACHKA1_001470"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO5679146.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            62462..63052
                     /gene="ribA"
                     /locus_tag="ACHKA1_001471"
     CDS             62462..63052
                     /gene="ribA"
                     /locus_tag="ACHKA1_001471"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO5679147.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(63116..65791)
                     /gene="acnA"
                     /locus_tag="ACHKA1_001472"
     CDS             complement(63116..65791)
                     /gene="acnA"
                     /locus_tag="ACHKA1_001472"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO5679148.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(65955..66050)
                     /gene="ymiC"
                     /locus_tag="ACHKA1_001473"
     CDS             complement(65955..66050)
                     /gene="ymiC"
                     /locus_tag="ACHKA1_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO5679149.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(66164..66331)
                     /gene="yciX"
                     /locus_tag="ACHKA1_001474"
     CDS             complement(66164..66331)
                     /gene="yciX"
                     /locus_tag="ACHKA1_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO5679150.1"
                     /translation="MVGQEQLESSPLCQDSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(66334..66474)
                     /gene="ymiA"
                     /locus_tag="ACHKA1_001475"
     CDS             complement(66334..66474)
                     /gene="ymiA"
                     /locus_tag="ACHKA1_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO5679151.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT        16217 a        17655 c        16414 g        16409 t
ORIGIN      
        1 gttcgggctt cgcacgttca aaccagtttt cggtatcata atcagcatct gtcgccattg
       61 tacgaagaat aaaaaataac caaaacgttg aaaggaactg agaatacgtt aagagaagaa
      121 ttaaattaat gtatcagcaa gtagtgctaa acagcacctt tatacttcag cctatctata
      181 aataatgaga atgccgagcc agcatgtcga cggtgtggat agtacaggtg ataaccgcgt
      241 aaatctggtg tgaatttatc caaaacacgt atcagttttt tttctttaat agcccgctca
      301 acctgatcat aaggtagata cgccaatcca tgcccatcaa ttgcagcatc aatgatcagg
      361 tctatcgtat tcagtaaaag ctgaccttcc atgcgaacac gaacttcacg tccaccgcgt
      421 attaatctcc agcgatttgc tgtacccgat gtgggaagat acaaattaat tgcctgatga
      481 tctattaatt gtgacactga cgttggaaca cttcggcgag aaaaataatc cggtgagcca
      541 acaatagcca ttggtatatc tggcccgatt cgaatggcga tcatatcttt atccatctcc
      601 ccacccagac ggacgcctgc atcaaaacgt tcagaaacga catcggtcaa accataatca
      661 atggtgagct gaatatcaat ttcaggatac gatttcagga atgtgcgcat tgctggtaac
      721 aatatcgttt ttgctgcatg ttctacagta gtaatacgta ttgtcccgga tgggcggttc
      781 tgcagatcgc tcagggatgt cagggcagaa tctatgtcat gcaacatagg gccaagaaca
      841 gacaaaagat gctcgcccgc ttcagtgggt acaacgctgc gcgtagttcg cgtcagaagc
      901 cgcaatccca atcgttcttc tatacggcgc actatctggc ttaaagctga ctgcgccatg
      961 ctcaggcggg ctgctgcgcg agtgaagcta cgctcctctg caacgacgac aaacgccatc
     1021 agatcagcga tttcttcacg cttcattcag tatctccata caatctatta atcatttaaa
     1081 tgataggtct aatcatgatt ttgggtctaa tcaacttaat gatcaatatc tatagtgata
     1141 tcaccaaccg ttaaaacgag ccaacaactc catgaggaac ggaatgatga ataataaagt
     1201 cagcttcact aacagcaata atccaaccat ctctttgtct gcagtgatct attttcctcc
     1261 aaaatttgat gaaacccgtc aatatccggc aattgtggta tctcacccag gtgggggcgt
     1321 taaagaacaa acggccggga cctatgccga aaaactggcg gaaaagggat ttgtcactat
     1381 tgcttatgac gcatcttatc agggtgaaag tggcggcgag ccgcgtcagt tagaaaaccc
     1441 atacattcgt actgaagaca tcagtgccgt gattgattac ctgacaacgc tttcttatgt
     1501 cgacaatacc cgaattggtg cgatgggaat ttgtgccggt gcaggatata cagccaatgc
     1561 tgcaattcag gatcgccgca tcaaggccat cggtaccgtt agtgcggtca atattggttc
     1621 gatgttccgt aatggttggg aaaacaatgt gaaatctatt gatgccctgc catacgttga
     1681 agcgggttca aacgccagaa ccagcgatat tagcagcgga gaatacgcca ttatgccact
     1741 ggcaccaatg aaggagtccg atgcgccgaa cgaagaactg cgtcaggcct gggagtatta
     1801 ccacacccct cgcgcgcagt atccaacagc accgggttac gctactttgc gcagccttaa
     1861 ccagattatt acctatgatg cttaccatat ggcggaagtg tacctgactc agccgacgca
     1921 gattgtggcg ggcagccagg cagggagcaa atggatgagt gacgacctgt acgatcgggc
     1981 atcaagccag gataaacgct atcacattgt tgaaggcgca aaccatatgg atctttatga
     2041 cggcaaagcc tatgtggctg aagcaatttc agtattagct ccgttctttg aggaaactct
     2101 gtaatgaaac atattcaaat ccgcaatagt gatatggcct ggcatattgc tgccaatatt
     2161 cagtttccac caaattttga tgagtcaaaa cagtatccgg cgattatcag cgtccatcct
     2221 tttggcagtt gtaaggagca gacgtctggc aatatctatg gcaaggcatt ggctgaaaaa
     2281 ggctatgtcg ttctcgctta tgacgccagt ttccagggag aatctggtgg ggagcctcgt
     2341 tggatagaag atccgacaca gcgcgtagag gatattagcc gtgttatcga ttatgcagta
     2401 actctgccat atgttgatgc tgaacgcatt ggcgtgctgg gtgtgtgtgg ggggggtggc
     2461 tatgccatta atgccgcgct gacagaaaaa cgaattaaag ctgtagtgag tattacgggt
     2521 gttaatatcg ggcgtctttt tcgggaaggt ttcagcaatt acgatccgat tggcgcattg
     2581 aatgcaatgg cgtcgcaacg aaccaaagag gctcgtggtg gagaattaca gatcaacgaa
     2641 ttacttcccg ccagcctgga tgcagcaaaa gcccatggac tgaccgaacg cgatgtttat
     2701 gaagcaactg actactataa aacaccacgc gggcaacaac cgggaggtgc gacaaaaatg
     2761 ttgttctcac atgcacagaa aacgctggcc tgggatgcct tcgcatttac tgaagtgtta
     2821 ctgactcagc cggtgatggt agtagttggt gaaaaagtcg gtgccttcgg tgcttatcgt
     2881 gatggactgg aagtctatgg ccgggcgatg gtttcgcagg atcgtcagtt agtttcatta
     2941 cccgatttct cacattatga actgtatgac aaaccggaag cagttcagga ggcgctggaa
     3001 aaagttattc cttttttcaa cacccatctg tgataagttt catgagaaat attatgaaaa
     3061 atgtactcat tcttggtgct ggcggtcaga tagcccgcca tgtgattaat caactggcag
     3121 ataaacaaac cattaaacaa actttatttg ctcgtcagcc agcaaaaata cacaaaccct
     3181 atccaactaa tagccagatt attatggggg atgtactgaa tcatgcagca ctgaagcagg
     3241 ccatgcaggg acaggatatt gtgtatgcca accttacggg ggaagattta gatattcagg
     3301 caaattcagt gatcgctgcg atgaaagcct gtgatgttaa acgtctgatc tttgttcttt
     3361 ccctcggcat ttatgatgaa gttccaggaa aatttggcga atggaataat gcagttattg
     3421 gtgagcccct aaaaccattc cgcagggctg cggatgcaat tgaggcttct ggactggaat
     3481 atacaattct tcgccctgcc tggctgacgg atgaagatat tattgattat gagcttacct
     3541 cacgcaatga accattcaaa ggtacgatcg tatcacgtaa gagtgtcgca gcattaatta
     3601 cagatatcat cgataaacct gagaaacaca ttggtgagaa tataggcatc aatcaacccg
     3661 ggactgatgg cgacaagcca ttcttcatgt aaatcaatta tatagccacc atcgtactgt
     3721 actatgtgca gtacgattta ttaaacttat taaggtgaac tgatcaatca gatgcgatta
     3781 acatgtgtag aaccagtaag aataaaatac gccatgtata taacattacg acaattctaa
     3841 tatctgcaca tcaatgaaaa tacaaacaac ttccggcaaa aaagtgatac aagatcgatg
     3901 acatagcgtt gaagaaaagg atttgataat ccacaaaatt tgcacaacac tgcctgtaat
     3961 acttcctgaa ccagagacga tcatgaccgt aacccgccca cgcgccgaac gcggcgcatt
     4021 tccgcccgga acagaacatt acggacgttc attattgggc gcgccgttaa tctggtttcc
     4081 ggcccctgca gccagtcgcg aaagtggttt gattctggct ggcactcacg gtgatgaaaa
     4141 ctcttcggtc gtcacgctct cctgtgctct gcggactttg acaccttctt tgcgccgcca
     4201 tcatgtggtg ctgtgtgtga atcctgacgg ctgccagctg gggttacggg ccaatgctaa
     4261 tggtgtggat ttaaacagaa actttccggc ggcgaactgg aaggaaggtg aaacggttta
     4321 tcgctggaac agcgccgctg aagaacgtga tgtcgtatta ctgacaggtg acaaacctgg
     4381 ctccgaacct gaaacccagg cgctgtgcca gcttatacac cgcattcaac ctgcctgggt
     4441 ggtctcattc cacgatccac tggcttgtat tgaagatccc agacacagcg aattaggcga
     4501 atggctggcc caggcgtttg aattgcctct ggtaaccagc gtcggttatg aaacgcctgg
     4561 ctctttcggc agttggtgtg cagatctcaa cttgcattgc atcaccgcag agtttccgcc
     4621 catctcctcc gacgaagcca gcgaaaaata tctctttgcg atggcaaact tgctgcgctg
     4681 gcatcctaaa gatgcaattc gcccgtcgtg aactgaagcg cctgttccac atctaccgcc
     4741 agccaggtcg gtccgtcaag atcggcgaaa ctgacctgcg gcaccagcgg taaagcggcg
     4801 ctaatggcac gagaggtaca caacatgcag cccagcatca gattgaaacc ttgtgcacgc
     4861 gcttcagtcg ccagcgccag cgcttccgtc agccccccgg ttttatcgag cttaatgtta
     4921 accatctcat agcgcccttt cagcgccttc agattgctac gagtatgaca actttcatca
     4981 gcacaaatcg gcaacggatg aataaaattc tccagtgccg catcgtcctg cgcaggaagc
     5041 ggttgttcaa gcatcgcaac gcctaaatcc gccaatagct ggcaacgtgc cgccaaccct
     5101 tctgcacgcc aggattcatt tgcatcaacg atcagcgtgg catcgggcac agctgtgcga
     5161 attgccacca tccgctcact gataagatgg ttatccagct tcactttcag taatttcgcg
     5221 ccggcctgcc agagtgttga tgcactattg gccatctgat caggcgtacc gatgactacc
     5281 gtctgtgcag tgataactgt ctcgggaagc gttatgccga tcaaatcagc cagcgattgc
     5341 tgctgttttc gcgcggccag atcccacaat gcacaatcca gcgcattacg tgctgcgccg
     5401 gcagggagaa tttgttgcaa ctcctcccgt gtcagccctt tctctaattg cggcacgacg
     5461 ctcataattt gcgccattac cgaggcatca ctttctccat aacgcggata cggcgtgcat
     5521 tcgccggtgc ctttaatacc ctcttcttcc agttcaacca ccaccacgcg cgcttcactg
     5581 cgacttcccc gggcaatcac aaacggggta tgtaagggcc aggcttcctc gaatacctta
     5641 acggttctca tctttactcc ttgttgtcgc ggtggtctgc ggattaagcg caaatagcgt
     5701 ttgctgtgct attgacagtt agcataaact aggtgtgacg ttaactatat gtaaacagga
     5761 aagataatca tgtcacaaac cgttcatttc cagggcaacc cggttacagt cgccaactcc
     5821 atcccgcagg cgggtagcaa agcgcagact tttactctcg tggcaaaaga tctgtctgac
     5881 gtcaccctcg gtcagtttgc gggtaaacgc aaagtgctga acattttccc gagtattgat
     5941 accggtgttt gcgccgcatc agtacgtaag tttaaccaac tggcaaccga gatcgacaac
     6001 accgttgtgc tgtgtatttc tgccgatctg ccgttcgccc agtctcgttt ctgcggcgca
     6061 gaaggtctga acaacgttat caccctctcc actttccgta acgctgaatt tctgcaagcc
     6121 tacggtgtgg caattgctga tggcccactg aaaggtctgg cagcgcgtgc cgttgtggtt
     6181 attgatgaaa atgacaatgt gattttcagt cagctggtgg atgaaatcac caccgagccg
     6241 gattacgaag cagctctggc tgtactgaaa gcataagtta atatgcctga tggtgttgca
     6301 ccatcaggca tattcgcgct tactcttcgt tcttcttctg actcagacca tattcccgca
     6361 acttattggc aatcgcggta tgtgaaacgc cgagacgttt tgccagtttg cgcgtgctgg
     6421 gataattgcg ataaagctgg gttaataccg agcgttcaaa acggctggtg atttcatcca
     6481 gcgaaccttc catcgcatct tcgcccacgg ctaccgttgc ggcgtcataa tccggcaaca
     6541 aaatatcctg tggacgcagt tcataaccgt ccagttgtgt cagtgcgcga tagatagcgt
     6601 tctttaactg ccgcacattt cccggccacg cataacgcgt aagtacagta ttcaggtcag
     6661 cggccagttt cggacgcggc acgccctgct cgtcggcaaa gcgggcgacg aacagctcgg
     6721 ttaacggcat gatgtcctgc gggcagtcgc gtagcggcgg cagattgagc gtcaacacgt
     6781 tcagacgata atagagatct tcacggaaca cgcctttttg caccagttcg accagattct
     6841 tctgcgtagc gcaaatcacc cgcacatcga catgcacctc atggtcttcg ccaacccgac
     6901 ggaaagtgcc atcattaagg aaacgcagta atttcgcctg catccgtggt gacatctcac
     6961 ctatttcatc caacagcacc gaaccaccgt tcgcctgctc aaagaatcct ttcttccctt
     7021 ccggagcatg accaaacagt tcactctcga ccgcatcttc cggtatagac gcacagttca
     7081 gcgccaggta aggtttgctc gctcttgggc ttgcctgatg gcaggcgtag gcaaaaagat
     7141 ctttacctgt acctgtgtca cccgtaatca gcagcggcgc gcttagcatc gccagtttct
     7201 gcgcctgttc gacaacatgc ttcattttcg ggctgacagc gacaatttga ctgaaggcgc
     7261 tgacgtcctg ggcggcgaca ttttgcaact gacggcccat acgaatcgtt gatcgcaaca
     7321 tcaccaccgc acctgtcagg acgtgttgat cattttcatc ctgaagataa acaggcgtaa
     7381 tctccatcag gaagttctgt ccattaataa caacatgctc gttatgggaa tcttgcggtt
     7441 cgctttccag ccaacgtaaa aaattaaagc cgttaatcaa ttgtgcggcg gtatggttgc
     7501 gcaggcgatc caatttttgc ccaaaaagct gacagctcgc cgggttcgcc atatccactt
     7561 tgcttttcat atcgacagag agcacaggtt caggcaacgc ctccagtaac gcgctcaacg
     7621 ccagatgctc acgttcggaa ggcatccacg ggacagtacg cacatcggta acacctgcaa
     7681 tacggcgtat ttcggccatc agactgctga aactctcaaa ctccagttca gcaaaattga
     7741 ggtagattcg cccaatggga tcaatctcaa taccgcgtaa atcaatgcct cttagcacga
     7801 gtagatcgag taattcgcgg gtcagaccga gtcggtcttc acaaaagact tccagacgca
     7861 tgggaacctt cacctgaaaa aagaacaatt aatatgatga tatgacacta tttggatagc
     7921 aggaaggttt ctgtcaacaa tcattgacac aaagacggaa aaacgggcac ttcggcgtaa
     7981 agatatccgg tcagcacccg atattgcatt atttttcgct gggcgttttg cctttttgca
     8041 gcgtttcttt cacctgaccg ataagctgac gacggaaatc cccgaggcga ggtttgtcat
     8101 cgtcaatcca cggcagcggg cggcaaagct ccatagcttt aatcccgagt cgtgccgtca
     8161 gaagcccagc accaatcccc tgagctgcgc gggtagacaa acgagcagcg agatcttgcg
     8221 acatccagtc catccccact tcgcgcacca attcgctggc tccggcgaaa gcgatattca
     8281 gcaataccag cttgaacagg cgcaaacggc tgtaatatcc cagttcaatg ccatacagcg
     8341 tggcgatgcg attaatcaaa cgcagattgc gccaggcgat aaatgccata tccaccagcg
     8401 ccagcgggct gaccgcaatc atcaacgttg attccgctgc cgaacggctg atttcgcgcc
     8461 gcgcctgggc atctaaaact ggctggacca aatgcgcata caggctgacc acttcacggt
     8521 cgttttgcgt ttcatggatt gaggcatacc agcgttgcag cgccggatgc gactgatcaa
     8581 tacccgcctg ctgcgccagt ttttcgcaaa atgcgcggcc tttgccggtt ccgtggctat
     8641 gcaataaatc acgcgcttcg tcgcgttcat gggcgcgctg tcgcaagcgc cataagcgcc
     8701 gccactctgt taccacagaa cctacgccag cgccgataat caatgcccca gcggcacatc
     8761 cacccagcgc cacccagtcc tgagtttgcc aggcattcat tgtccactgt ataccctgcc
     8821 cgacaacgct tgcgccaaac agagccagcc cgcccatcac cattttgcgc cacaggctgc
     8881 gtttcggacg taacgctgcg tccattaccg cttcgacttg cccctcttct tcctgcgctt
     8941 cgtcgagcgt ggccggggca aaattttgca cctgattttc gtcaaaggtc tgctgcgccc
     9001 tgaatttagg attctgatcg acctccagag gaccgtcgaa atcaatacgt ggttttaacg
     9061 gttcggtcat cgcaatttat ctcctattaa aaattccagc gcggcatcaa gacgaatatg
     9121 cggcagcggt ttgtcgacat ccatcacctg cggacgaaac gcctcaaact ggaagccttg
     9181 cttatcccag aacgcctgac caggcaaacg tgcggggact tcgccaggat aaacagtgag
     9241 cggtgcgcca tcactaagtc gattaccgcg cagcgccggg attttctcac cgttaacatc
     9301 aataatgccg ctggtggtcg cctgaacaga cgccagcccc aggcagtcca tgctgatccc
     9361 ttcgaacgcc gcattttgcc aggcatcctg aatcagttgt tgcagtaatg aaaccatatt
     9421 agcgtgctga tcgatggtca catggtccgc tttagtggca gcaaacaata gcttatcgat
     9481 aaccggcgaa aacaaacgcc ggaacagggt acgctgcccg tagtgaaaac tttgcatcag
     9541 ttgcgtcagc gccagacgca tatcattaaa tgcctgtggc ccactgttga gaggttgcag
     9601 gcagtccacc agcacaatct ggcggtcaaa gcgcagaaaa tgattcttat agaacccctt
     9661 caccaccttc tcgcagtaat aattaaaccg ctcgcgcagc attccggcat tggtgtgctt
     9721 atcggcctgc gccagtttgg actcgcccca ggcatcgaca tccggccacg ggaagaattg
     9781 cagcgcgggc gcacctgcca tatctcccgg caagacaaag cgccctggct gaataaagtg
     9841 cagcccctgc tgtttacagt ggtggagata atcggtccac gcggcggcaa tgtctgccag
     9901 ccggttttcg tcggcaggcg ctagcgggtc cagcccttcg cacatcattc gccatttcgc
     9961 cgaccattcg ccgcgctgac cattgagtaa gcccgtcatc tggcgcgacc agcttaaata
    10021 gtcctgcgcc agcatcggca ggtcgagcaa ccattcgccg gggtaatcca caatctccag
    10081 atacagcgtg gaggtatcct taaagtggcg cagcagcgaa tcgttcgatt tatagcgtag
    10141 cgccaggcga atttcgctga cgccgcgcgt tggcgtcggc caggcgggag gatcgccata
    10201 cagctgcgcc aacccttcgt cgtaggtaaa acgcggaatg ccaaagtcac gctgaggaat
    10261 gcgttttacg cccagcaggc gctcttcacg caccgcactt aacagcggca aacgtgctcc
    10321 ggcatgaata ttgagcaact gattaaccat cgcggtgata aacgctgttt tgccgctgcg
    10381 gctaagtccg gttacagcga ggcgcagatg tctgtcgaca ccccgattca ccagcgcatt
    10441 aagttcattt ttaagtcgct tcattgctgt cctgtgctgc tctgaagacc agaaaaaaat
    10501 cattcataag gatatgaatg tgggggccag gccattgatt atcaactacc cgtgttgtca
    10561 tcgacgggcg aactccataa gatgtgcagc attcacgttc ggacggagtt cctgacctat
    10621 gtcccctact atttatgata ttgccagggt tgcaggcgta tcaaaatcca ccgtatcacg
    10681 cgtgctgaat aagcaaacca atatctcccc ggaagcgcgc gaaaaagtgt tacgggccat
    10741 tgaagaatta cagtatcaac caaacaagct ggcccgcgcg ctgacctctt cgggttttga
    10801 tgccattatg gtgatttcta cccgttcgac caaaactacg gcgggtaatc cgtttttctc
    10861 tgaagttttg catgccatca ccgccaaagc tgaagaagaa ggtttcgacg tgatattgca
    10921 gacgtcgcac aacccggcag aagatctgca aaaatgtgaa agtaaaatta agcagaaaat
    10981 gattaaaggc attattatgc taagctcacc tgctgatgaa tcgttttttg cgcaactcga
    11041 taaatacgat attccggtgg tggtgattgg caaagttgaa ggtcaatatg cccatgttta
    11101 ttctgtcgat accgataatt ttggcgacag cattgcgttg accgatgcgt taattgaaag
    11161 tggacatcaa aatattgcct gtctgcatgc accgcttgat gtccatgttt cagtggatcg
    11221 ggtaaatggt tataagcaga gtctggaaga tcataatatt gcagtgcgtg atgaatggat
    11281 tgttgatggc ggttataccc atgaaacagc actgcaagca gcacggcaat tattaagtca
    11341 gtcaccgttg cctgaggcag tgtttgccac tgacagcctg aaattaatga gcatttatcg
    11401 tgcagcggca gagaaaaaca ttgctattcc gcatcagtta gcggtggtgg gttatagtaa
    11461 tgaaacgctg tcgtttatat taacgcctgc accgggcggc atcgatgttc cgacgcagga
    11521 gttaggtcaa caaagctgcg agttattatt ccgcttaatt tctggaaaac cgtcaccaca
    11581 gaatattacc gttgccacgc atatgtcgtt gaaataaatg tatgccggat ttggcgctcg
    11641 cgccgcatcc ggcaatttag cccattatca gaacgagtaa tttacgccga cacctgcata
    11701 atggaattta tcactgtctc cttcgtcgtg atcctgccac tcaaacgcgt attccagcga
    11761 aacggaaaga ccattctgga aatcataacc gtaaaataaa ccaacacggt taaaatcatg
    11821 gccttcacgt tcaatatcat cctgccagtc ccagttactc cagcgatcca gcccaatgcg
    11881 cgtatacggc gtcaccgagt ggttgccgag cgtcagcggc aaataggcgc gaatttcttg
    11941 cgtggaaaac tcaccgttat tgcggctgtc gtccatattg aagccgcgct cgagataata
    12001 gttcactcgc aaggcaaccg tttcgttgaa ggtatattgc agacctgttt ccgtttcgac
    12061 acgggtatca gcgtaaccgg tcgtgttcag atcgttggca aatttataca tcgacaacca
    12121 accgttgaaa cgtaaatcgt cagtcagttt cacatcccag tctggcgcga ttttccagcg
    12181 ctgcatattc gccgtatctt tacccggttc atcaacgtag tgataaccat aattacggaa
    12241 accgccggtc aggccgaaac tgaaatcatc gctttcgagg aactgataat gcacctccag
    12301 ctccgggcga tcaaaccacg ttccacgttt acccgcgcta taatctaccg gcccttcctg
    12361 ataataggcc agagcaattc tccacggccc gttggcggca ttaaaataga ctgaaggctc
    12421 cgccagccca tccatatctt cgccataacc ctcgacgttt tctatttcgt acatcgcgcc
    12481 gatattaaag tgccagtcgt tcttttcctc ggcctgtgcg caggccattc ccgcacacat
    12541 caccagtgcg gtacagggta ataacttttt cattattgtt ccttgtatta ttctggggga
    12601 atgccttccc cctgtattta gcgaattgct atttccgttt ctgcatcaaa gaaatgacat
    12661 ttcgtcatat caaaatgaat agtgatattt tctcctgcat gataatcatt taacgcacca
    12721 gcacggacca ctaactcgtg ccccccaacc gtggtgtaga gcataaattc cgcaccggtt
    12781 aattctgcca cgctaatttt ggcggaaatg ttattttcct cttgcgcgtc cggatgaata
    12841 tcttccggac gtattcccat cacgatgggc ttatgcaaac ttccctgtgt tttcagaacc
    12901 gctaatttct cttcgggaat ggttaattta agcgtttccg taacgaattt atcgccatcg
    12961 atcgtgccac gaataaaatt catcgccggt gatccaataa atccggcaac aaacatattc
    13021 gctggttggt tataaaccgt tttcggcgca ccaacttgct gaacaatccc atccttcata
    13081 atcacaatcc gcgtcgccat ggtcatcgct tcggtctgat cgtgggtcac gtagatcatg
    13141 gtggtattca gcttctggtg cagcttgctg atctctgcgc gcatttgcac gcgcagcttg
    13201 gcatcaaggt tagagagcgg ttcatccatt aaaaacacgc ccgcttcgcg tacaatcgcc
    13261 cgcccgagcg ccacacgctg gcgttgcccg ccggaaagcg cgcccggctt gcgtttcagg
    13321 tactcacgca ggccgagaat ttgcgcagcc cagttaacgc gctcatcaat cacctctttg
    13381 gcgatttttt gcatcttcag gccaaacgcc atgttgtcgt agaccgtcat atgcggatac
    13441 agcgcatagt tctggaacac catcgcgata ttgcgctctt tagccggaac gtcattcatt
    13501 cgtttgccgt cgatcaacag atcgccgccg ctgatctcct caagcccggc aatcatgcgc
    13561 agcgtagtcg atttaccgca gcctgacgga ccgacaaata cgatgaactc tttatcggca
    13621 atttccaggt tgaagtcctt caccacatgc acctggttat cgtagatttt ttgaatatgt
    13681 tgtaacgaaa gctgagccat gttgattcct ttgctatacg ttttgccaga aggccgataa
    13741 ccgcgaccag gtcaggaagt ccgttgaggg caacagtaat tgcgccccgg ttaagcccgc
    13801 gccgatcccc accgagcgca taccactggc gttaatggcg tcaatgcccg cctgcgcatc
    13861 ttcaatgccg atacatgcct gcggcggcac gcccagcccg gcacaggcag cgagaaagat
    13921 ttccgggtcc ggtttcgagt ttttaagttg ggaagcgtcc gcgcagaagg tgaaaaactc
    13981 acgtagctcc aacgccgcta aaatcgtcgg cgcattcagg gagacagaag ccagccctat
    14041 agggatctgc tgtgcgcaga gatctgccag caaagagcga atgccgggca acacagcatt
    14101 aaccgtcagt tcgcgcagtg agtggacgta gagcagattt ttgcgatacg ccagttgcgc
    14161 ccgctcctgc gggttaaagt cgccctcttt gtccccgtgt tgcagaatgc gccgcagaga
    14221 ctcatcgcgg ctgatccctt ttagggattc gttaaactgc gcatcaatgc tgatgccaat
    14281 ttcagcagca atctgctgcc acgccttgaa atgcagatgc gcggtatcgg tgattacacc
    14341 atccaggtcg aaaactactc cttgcagttt cattcatcct cccgatgcgt ggtagcggtc
    14401 ccattgaagg gcaaaataaa atcgcccaaa cagaaaacag attcttcagc cacggatata
    14461 agctggccgt tcaaacgcag tgaaacgggc gcagaagttc gaatcgcaat acgctgcgcg
    14521 tcgagcgtga cctgtaattc gcagccctgc cagaacagag ggaaagacaa ctgttgccac
    14581 tgctcaggta acgccggatt gagatgcaat tcaccgtcac gcacgctcaa cccggcaaaa
    14641 ccctgaatcg cccccagcca gatagcgcca gttgcggcag cgtggatacc atcatcacaa
    14701 ctatgcggat cagcaccaag atcgatttca gtcccctcgc gccagaactg ataactttgg
    14761 gtcagcaggc cacagcgtgc ggcaacaatg ccgtggattg ctttacttaa tgacgagtcg
    14821 tgaatagtgc gcggttcata aaattgcaga ttggcaagac acgatgccgc tgagaactgc
    14881 tccggcagca tgtaattgag catcaccaca tcagcttgtt tgaggatctg catctcgttc
    14941 acttctgcgc gtgaataatc cagcagaatg gtttgctttc ccgccgccgc tttgtatttc
    15001 gccaggttaa tcgccggctt agccataaac gaatcatcct gcggcaaaac gccgtcgggc
    15061 tgaatttctg gcagccgcag ttctttaagg aacatttcgg cgcgatggat aaacgcatcg
    15121 tcgctacagc caaattgacg agcgatgttc agcgcctgct ggacgttgta atacgccata
    15181 tagctggtga aggcgttatt attgacatgt tcggtatatt cgtctggccc aataacatca
    15241 tgaatttcca gacgatcgtt aactctcacc gcgcggctaa tccagaactt tgccgtctcc
    15301 agaagtagcg ccatgccttc atgagcaatg aaactttcat cccctgtggt ctgccagtat
    15361 tgaataaccg cccaggcgat atcggcgacc agatgatgtt ccgcctgcgc cgaagccact
    15421 ttttgccgca gcccggtgcg aatgttaatg gcggcaaatt ccggcgtctc ttcttcgccg
    15481 ctgcgcgcgc tttcccacgg aaatagcgcg ccctgccagc cgttgcgtcg cgctttctcc
    15541 tgcgcgcctg gcaagttgtg ccagcgataa cgcagtaaac ttcgggcaac cgtcggatcg
    15601 ctaaacagat gaaacggtag caaaaatact tctgtatccc agaaaacgtg gcctttgtag
    15661 ccttctccgg ttaagccttt tgccgcaata ctgctgcgct cgtcgtgagc aggcgtcatg
    15721 atgcgcagat gataaagcgc ataatctaac gcttgctgat cgtgcgcatc gccgccgttt
    15781 accgtgatac gacgtttctg ccaccattga cgccagtttt ctgttgatgc tgcaagaagt
    15841 tggtcgtaac tctgctgcgc gcacatttca agcaggcaaa gcgacgcgct gccccactcg
    15901 tctaaaacgg cttgcctgtc atcccgccag tcgatccaca ccagtttttg caacgtcact
    15961 gtctcgcctg catgaagctg cgcactggta tgttgcagca aacggcgctc tttagccgta
    16021 tagcattgct gaacgtcacc actcacctgg cagcaacagc taattgccac atcactgcgt
    16081 ccatcctgag tggtatagat tccttgcatc aaatgctgtc caaacactcg cacctgggtt
    16141 tcgtcgagat gctggcgacc gtggttggtt tgcgtggcgt caatgcctgt tgaaatcagc
    16201 actgacgcgt cggcgtccag tggagtaata gtgatttcca gcgcaatgag cggcagttgg
    16261 tctgccgaaa caaagcgacg gctggcgatg gtgtaacctg tgccgttgct ggtacgccag
    16321 acaacgctgc ggcgtaattc gccactggca aagtcaagct cacgctgcca ggcttcgcgg
    16381 gataatgaga aaatctcacc atttatggca atctccatcc ccaccacatc gggcaggttc
    16441 accagttcgt tgatttcacc tttacccgcc cgatgataca gccccgccag atacatgccg
    16501 cgcgtctggc gcgtgtaatc ttcttcatgg ctggcgcgaa gcccaagata gccgttcccc
    16561 tgcgccatca gtgatgcata cttgttcagg gtatgctggc tgaaatgggg ttctgataac
    16621 gttactggcc tggtcataat tccacacgcg tccctgtttc tgcggattga tacaacgccg
    16681 ccaccagttg ctggatgatg tacccctgct cggcatcggc tatcatcacg ggcttgccct
    16741 gtacgtgatt gataaaggct tccatgctgc gcaaatggcg gttgtcgtct gccatttccc
    16801 gttgcatcag cgtcattaac tcaccgttgt tatcggtgta gatatgtgct ggaaacagcg
    16861 tcgcaccggc tttatcacca caaaagctga cgttcataat cgactgttcg cggatgttga
    16921 gcgcaaatga cgtttccagc cacagaatgc cgccgttatg aaattcaatg gtgccaaaca
    16981 gcgaatcttc gacgctgtaa gttgccggat cccactcgcc aaattgacca cagctctttt
    17041 gcgtgccgat cttttgaaag ctatgcgcat tcacgctttt caccgccgga aagcccagca
    17101 catacatcgc cgcatccagc atatgaatac cgatgtcgat cagcgggcca ccgccctgca
    17161 gttctttatt ggtaaagaca ccccagccgg gaacgccgca gcgacgcagg gcgcgggcgg
    17221 tggtgacgta aatttcgccc aggatgccgt tagtcacttg ctcacgcagt tgttgtgtat
    17281 caagcgcaaa acggtgatgg aagtcgtagg ccagtacttt tccttgtctg cgcgcggtat
    17341 cgcgcatgac ccgagcctgt tccggcgtca tagcaggcgg cttttcgcac atcacatggc
    17401 agccggcttc cagtgccata agggtatgtt catagtgaaa acggttaggt gagcagacgc
    17461 taaccacgtc agggttcacc gccagcagca tggcctgcgg atcgtcccac acggatgcat
    17521 taccgtattt atctgccagc gcctgcgcct gggaaaggcg gctgtcacag acagccacca
    17581 gttccagatc gttgcgggtg cagtagtacg aagcatgaac tttatccgcc acctggcccg
    17641 cgcctatgat cgcgacccgc agcggagagc ttgtcattgc acttttcaca aagacctctt
    17701 agcaggtacg caaccaggcc aaagaatcac ggtacgcctg ggcaggatct tctgcccgaa
    17761 tgcgcccttc atacaccaca tagccctgat aattatcggc gcgcagttgt tcaaacagcg
    17821 cgtggaaatc cagggtgccg ctgcccggct gataacgatg gttatcggca atatgcacat
    17881 gaccgagcag gtcgcggttg tcatgcagcg cctgcgccag gttatcttct tcaatattca
    17941 tgtgatagaa atcgccgata atctgcacat gtttaagatc gttttcgacg atgtaacggc
    18001 gggcatcggc gagagtgttg atcatgtgat cctgataacg gtttaacggt tcgagataca
    18061 ccacggttcc ggtacgcgcg gcgacctgtt ccagtacgcg cagggaatca ctcaccattt
    18121 tacgatcgcc gtccaggctg cgcggcgagg tcatcggcgg taagcggaag gtaaacatgc
    18181 cccacgctgc cggaacgacg atacctttac cgcccacttc tgccagcact tcgagaatgc
    18241 gctctatctg ctttaagcca ttaagacgac gctcttcgat aaagtcacca atccagccgt
    18301 catagccacc gcaggcggtg gtcaccggta aaccggtttc tttgattgcc gctttgactt
    18361 cttcgatgtt gttgaccagc agtttgccgt caatctcaaa accatcgaag cccatctctt
    18421 tgatataacg aaatttctcc agaatatttt ccgggaagaa cgcctgattc tgtgtgccta
    18481 ttttcatttt ttcattcctt tgaattaaaa cgtgacgccc attttgatgc tctgttccgg
    18541 atgctggtcg acatactgca tgtagctttc cgggctgtta gcaaaggtca ccaccgggtc
    18601 gattaaatcc tcacagttga gataaccgtt catcagcagc tcccagcagg tttcttcgat
    18661 acgcttgcgg ctccagcgcg gataatccgg gttcggttcg ctgcacgcgc gggagaaaac
    18721 aattttggcg ttattgaaat gcgcttcgcg tccgaggtta aaaccttcgg caaacggttt
    18781 agcaaacgcg acataggaga tggtgccacc gtaagccagg ccgcgcagcg ccgactgcag
    18841 cgcgtcggcg taaccgctgg tttcgataat cacatcggca ccctgcttgc cggtcagcgt
    18901 tttgatctct ttgcctacgt cagtgccaat gggattaagg cagaaatccg caccgtgacg
    18961 acgagcaata tcacaacgat gggcaatagg atcgacgcca attaccaccg acgcgccagc
    19021 gcgtttagcc agttggatgg caatttgacc gatcgcgcca agccctacca ccactacaaa
    19081 atcccctacg cgtacgttgg catcgcgaac gccactcatg gcgaactgcg ccgggtcgta
    19141 gcagacggcg tttttccagg agctgccttc cggcattttg cgcaacttgt agttattcac
    19201 tgcgttaatg atgaccgtct cggagagcgg gccgtagccg catacgctgt cgcccaccgc
    19261 atagtcggta acgtcgctgc cgcactcgat aatgtcgcca accaccatgt tgccaagctg
    19321 gaatttgcca aactcaatgc cgcgcggcgc acctgccgga cgcggggtga acatctgcca
    19381 ctcgccgtta aagtcttcat cgataaacgg gctggcggcg cggaagtcga ccacttccgt
    19441 tccgtgtttc ggtgcgccga aacgcacgcg gatcttcact tcattggcta aaatcgcccg
    19501 atcttcatac tcaaccagcg cagcaacacg cggtgctgtg gctactaact ttttcatgta
    19561 aaactccttg taaagaaact ggccgcttta gcccttcacg ccaccggcgg tcaaaccact
    19621 cttaataaaa cgttccgaaa gcgcatacat aatcaccacc ggcaatgcgg tcaccagcga
    19681 ggccgccatc atccgtcccc agatgtaatc aggcgtactg aacagcgcgt tcaggcccac
    19741 tggtaaggtg aaattgctgg cgctggagag gaaaatcgac gcaaacagat agtcgttcca
    19801 cgccaccata aagcagtaga cgaacaccga tatcagcccg gacatcgcca ggggcacggt
    19861 gatacggaag atgatttgca gacggttgag gccatccatc atcgccgctt cttcgatctc
    19921 atcagggatg gtgtcgaagt aacttttcag cataaacacg gcggtgggca gagtttgcgt
    19981 caccatggta atgatcagcg ccatctcggt gtcgtaaatg ccaagtgcgg tgataatttt
    20041 gaacagcggc accaccagca aaatgccgga gaacatgtac accgtgtaaa agctggcgtt
    20101 gatggtcatc cgacctttaa agcgcaggcg agaaagcgcg taagccccga gaataccgag
    20161 aaataccgcc accacggatg aaaccaccga caccaccatg ctgttacgga agtagtcgac
    20221 aaacgggaaa atcaccgggt taaaaatgtc gacgtaatgc tccagcgtcc actgctgtgg
    20281 cagcagcgta ggatgcagcg agatcgcctc ttttgcgctc ttgaacgagg tcatcagcat
    20341 cacaaaaaac ggaaatagcg tgatgatgag aaacagcgcc agcccgcagt aaaaaccgat
    20401 gcgactgagt gtgcgtttat ttgttgccat tgaggttcac ccgttttctg gtcagcagaa
    20461 tgacagcgaa aataatgatg aatagcacca ccgagatcgc cgccgctttc cctaagtcat
    20521 taaaggcaaa ggcggttttg tagagataga caccaagaat atcgaccttg gttgtcagca
    20581 gataaacatc agcgaacatg tagaacatcc agatggtgcg cagggtcacc accgtcgcca
    20641 ggaccggcat aattgcgggc agcgtgacga tgcgaaaccg ctgccaggca ttagcgccat
    20701 ccatctctgc cgcttcgtac agcgatttgt cgatggtctg caaaatcgcc agaaacgaga
    20761 taaaggcata tgggaaatag cgccagatgg cgaacagcac caccagcaca aagctactgc
    20821 ccgggttatc gaaccacagc ggtgcctgct cgtagagatg cagaagatcg acgccgaggt
    20881 agttcacaat gccgtagccg ttgttgaaca tgtatttcca ggcgaacacc agcgaaatgg
    20941 acggcgttac gtaggagagg atcaccagcg aacgcgcggt tttgcgcagg cggaactcgc
    21001 ggttgaaaaa catcgccacc gccagcccga gcacggtgct gcccgccacc accagcgcgg
    21061 tataccagac cgtcatccac agcgaatgcc agaagccggg atcggagagg atacgcacat
    21121 agttgctcac cccaacaaac gttgactcga tgttgggatt gagcggcaga cgtaaaaaac
    21181 tgatttcgat attcgacacc atcggccacg ccaccagacc gcccagcagt aataagctgg
    21241 gcgcgagaag cagcagcgca aagggcatat cggaacgacc tgaaaaaagc ctgttcattg
    21301 cggattcctg tcctgggttt agtgctgttc gatcagttca tccagttttt tctggctcgc
    21361 ttgcagcgta gtagagagat cggctttacc cacggtgacg ttatgcacca ttgaactcac
    21421 cacgccagaa cccgtcacat cacccatgcg ggtaaagttt ttatccccta ctgcgccaaa
    21481 aacctgaata tttggcagtt caccaattaa ctgattcggc agttcaccca gcgccttgat
    21541 cacgtcgttg tctttccagg tggcggtagt gacaaccgct ttgttcaccg gcagcgccgc
    21601 gcctggcgac atcatcaccc agtcggcaat gttgtctgcc tgctccataa aggtgacaaa
    21661 tttttctgct gcttcggtct cttcggtctt ttgtccggcg gtaatggtca gcgaggtcaa
    21721 catgccgtag accgcagagt ttttctcggt tggcaccacg aaaccgacgt ttttcgggtc
    21781 gccttcttta attacagccg gaaggatata ggtggagtaa atcgccatcg gcgcggtgcc
    21841 gttcataaag gcgtctttca cttccatgat gtcgttagaa cccggcatgg tgttggcagc
    21901 aaggtcgcgg taataggtca gcgcctgcat catctctggt gtatcaaggg tgattttgcc
    21961 ttcggcgtta aagacgttgg cctggttgga taacgcaaac tgggagaagg attgttccgt
    22021 cagcacgctt tctgccgtag gcagcgcaat accgtatttt ttattcgccg ggtcattcag
    22081 tttctgtgca acgtccagca gctgttgcca gtttttcggc tcctccagcc cggcttttgc
    22141 cagcacatct ttgcgatacc agatgccgcc aatccaggcg ctgacgggaa caccggtcca
    22201 ggcactccca tcttcggtgc gcacaatacg cagtacgcca tcgtaaaacg cgccttcacc
    22261 aacgttgctg atgaccgtgg caaccgcctt gcgatcgata agctgctctt tgtccataac
    22321 tttggcgtag tcatggctgg tttcgatcac ttccggcagc gagccgctac gtgaaagggt
    22381 gatgactttg gtgttataag cgtcttcttc cacgggcact tgtttaacgg tgatacctgg
    22441 gttttctttt tcaaaacggg caatcaattt gctgataact gcctgacgct cctgctccac
    22501 cgaagaatgc ataaattcga tggatacatg ggttttattt tcttctttac agcctgaaat
    22561 cagggcgcag gaaaccagtg ctgataacag cacgattttt gatttaatca tgagatgttc
    22621 ctttttattt aatccacata acctgccagg ggcgcagcgt tattttatta cccaatatat
    22681 caacttcact aatcagatcc cgaccgtgca ggccagtaat atttacatgc tgaatatttt
    22741 tactgacatt aaacaatccg gtcagacaat taccctctgc gttacttctt tgaatacgca
    22801 ttacggatga attaaccgta tcgatggtaa aattattatc cggatgaaac tcgttatggc
    22861 tgcgacgaag tgtaattaaa cgcgacaact catgatatac cgcatgcctt aatgtagctt
    22921 catcgttcag ttctcgggtt atctcttcac tgtgatattt tttacggtta atcgcacggt
    22981 tatatccgag tttttcgaca cctgcgtaat cattacgcga gcccagaata ctttgaatat
    23041 atatcgctgg cacaccgggg aaacttaaca aaatcgcatg ggcaaggata aacctggcgc
    23101 aacgttcttc atcgctactc tcgcggcggc ttaacgcatc catataggtg acatttattt
    23161 catagggact gcgcgtaccg tcgggattat ttttccagtt tactaatgca ccttcctgct
    23221 gtaatgcctc gaccagcgct aatatttcgc tttctggtaa caagccccgc agtgggttca
    23281 gcccgatgcc atcgtgagag gcgaggaagt taaaccaggt cgttttaccg gagggcaagc
    23341 tcagactttg cgcccactgg cacagcgcct cgacgttctg tttttgcacc gcatgcagca
    23401 ccagcggcgg cagcgagaac tgatacacca tatgggcttc atcatcgccg ttgccaaagt
    23461 aagcaatgtt gtctttatgc ggaacattgg tctcggtaat gatcactgtg cctggcgcga
    23521 cgttatcaat aatcgaccgt aacagtttga taatcagatg tgttttttcc agatggatgc
    23581 agcttgttcc cggctctttc cacataaagc caacggcatc cagacggaca tactctgcgc
    23641 ccttttccag gtagcacagc aatacatcca ccatcgccag caacacttca gggctacggt
    23701 aattgaggtc aatttgatcg tcgctaaagg tggtccataa atggcgcgtg gaattatcat
    23761 ccatttgaaa cggcgttaat agcggtaacg cacgcggacg ggtgacggca ctgagatcgg
    23821 tttgcggatc aaccgcaata aagaaatctt caaaacccgg aagctgttgc aaatagtttt
    23881 taaaccattc actttttgcc gacatatggt tgcagacaaa atcaaacatt aaatgactgc
    23941 attcaccgag ctgctgaata tcctgccact cccccgcttc actggcgacc tgatgataat
    24001 caattaccga aaatccatca tcagatgacc atggataaaa aggtaataaa tgaacatgtg
    24061 aaaaaatgct ttgcagccat tgatggtaaa actgattaaa tgtgggtagt ggttttaaat
    24121 cattactgtg aaattgatcg gcataggtaa ttaacacgac gtcactttca tcccagtgtt
    24181 ttttacgtcg ctgtgtaatt aatcgtttcg cactctcaag acgcgttaca agtttctgta
    24241 aatgagttga ggtaaatgtt ccaccgtaaa tttctgccaa gtaatccgta atttcctgtt
    24301 tcatgtgcct ttccgtggta gcggtcccat gaaacgagag actactccgc agaaaataaa
    24361 ctgtcaattg agcacaaggg ttttagtcac gcaggaaatc attttttacc agggaaaaag
    24421 cgtatttgtg agctgacgca aataatctat agggatgtaa ataaggtgtg acagaaaaaa
    24481 acggcgcata agcgccgctc atggtgaatt cttttaacct ttgaccttcg gcattgcgat
    24541 gtctttcagg ccaccggcgt tctcaacgtg ggtatatccc atctcgctaa ggatctcttt
    24601 tgcctgcccg gactggcgtc cggcattgca atacactttc acggtgtcgt ttttatccgg
    24661 aacggcggtg gcaatgcgct ctttcacttc cttcagggga atattgatgg ccccctgaac
    24721 gtgctcctgc tgatactgtt ctggaacacg aacatcgatc cagtgttcag cggcgaaaac
    24781 gggcagtgaa aacaccagtg tcagagcaag taagcctttt ttaaacatgt aagcgtcctt
    24841 ctatggatga aaattgccat ccattatacg cctgagattt aacaattttt gcgaaactcc
    24901 cctcaccttt tataacgctg cgccagttta ttggcagccc gacttaacag cggttccagc
    24961 gccacagcca gcaacatttt cagcggtcgg cgagcaactg attttatcgc ccagcccgcc
    25021 acacccgctg ggccatagcg cagtgcggta agaagtacca gcttgcctgc taatttgaaa
    25081 ccaggcttta ccttttgccc ggcctgttgc cagcgagtat tcataacttt cctcacagtt
    25141 gacggaaacg gctacgtaac gtgaaagtat cggaagtgac ataacgttcc atctcgcgta
    25201 aacgcgtttc acttgccgcc agttcacggt cgacttcatc cagcaattcg ctgctggaag
    25261 gtagctgctc accaaaggcc atgttgtccg gcattggatc aagcgcaaat gacaaaatga
    25321 tgtaagcaac cagggtaaac agcgccagac cgaagaaaat cgacagcacc accaggatac
    25381 gcaccagttt taccggtaca tcaaaatagt tggcaatccc ggcgcagacg ccacggacca
    25441 tgccctgctg tggaatacgc cataattttt tattgagatt aatgcccgcc attagcgatc
    25501 cctccagttc ggatgttctg catcaagaat agattccagc gcctgaatac gttcgcgcat
    25561 ccgttttgct tcatcagcca gttgcgctaa tcgctgctgc tcactttgcg acaattcact
    25621 gcgaccagaa cgattgctgt aatgcagcca taaccagatc ggtaaaacaa acagcacaaa
    25681 aatggttaat ggaatagcca gaaatagcgc gctcataagt actccttaga cgatgagcgg
    25741 cggcgcgttc ggacgccgcc tggatgttat tattgattgt cttgcttcat tttggctttt
    25801 aattgtgcca gttgctcgct gattgcatca tcggctttca gttcggcaaa ctgatcgtcc
    25861 agcgattttt gtttaccgaa gctgtggctt tctgcttccg cttccatctg gtcaattcga
    25921 cgttcgaaag attcgaaacg agccattgct tcatccagtt tgccactgtc cagctgacga
    25981 cgcacatcgc gcgacgagtt tgccgcctga tggcgtaaca tcaatgcctg ctggcgagca
    26041 cgggtttcgc tcaatttgtt ttccagctcg ccgatctctt tcttcatacg caccagcgta
    26101 tcgtccacca gcgtcacttc atgttccagg gacttaatca gatcggtcag tttctgtttt
    26161 tcaattaacg ccgcacgcgc cagatcttct ctctctttca gtagtgccag ttcggctttt
    26221 tcctgccatt caacttcacg cgccgacgct tgttcaatac ggcgagtcag ctgtttcttt
    26281 tccgccagcg cacgcgccga agtagaacgt acttcaacca atgtatcttc catctcctgg
    26341 atcatcagac gcaccagttt ctgtggatct tccgctttct ctaacagagc gttgatgttg
    26401 gcattcacga tgtcggcaaa gcgagaaaaa atacccataa tattgtcctc ttgatttctg
    26461 cgttcaggat tgtcctgctg aactgttaat acaatttgcg tgccaatttt ttatcttttt
    26521 gatttataaa gatctgattg aagaatcaac agcaacatgc caggatgaat tagcgaatta
    26581 cactaacaag tggcgaattt catcatggca gaatacaaag ataatttact cggtgaggcg
    26641 aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg caccgctgga caaaccggtg
    26701 ctcatcatcg gcgagcgcgg cacaggtaaa gaactgatcg ccagccgtct gcattatctc
    26761 tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg cggcgttaaa tgaaaatctg
    26821 ctggattccg aactgtttgg tcacgaagct ggcgcgttta ccggtgcgca aaaacgtcat
    26881 ccagggagat ttgaacgtgc cgacggcggt acgctatttc ttgatgaact cgctacggca
    26941 ccgatgatgg tgcaggaaaa attattgcgc gtgattgagt acggtgaact ggagcgcgta
    27001 ggcggtagtc agccattgca ggtgaatgtg cggttggtat gcgcgacgaa tgccgatctc
    27061 ccggcgatgg tcaatgaagg cacttttcgc gcagacctgc tcgaccgact ggcttttgat
    27121 gttgtacaac tgccaccact gcgcgagcgc gaaagcgaca ttatgttgat ggcagaacac
    27181 tttgccatcc agatgtgtcg ggaaatcaag ctgcctctgt tcccgggttt taccgagcac
    27241 gccagagaaa cattgctgaa ttatcgctgg ccgggaaata ttcgtgaatt gaaaaacgtg
    27301 gtggaacgtt cagtgtatcg ccacggcacc agcgattatc cgcttgatga catcattctt
    27361 gatcccttta aacggcgtcc accagaagaa gccaacgccg tttcagaaaa cacttcgctt
    27421 ccgatgctgc cgctggattt acgtgagttt cagatgcagc aggaaaaaga gttgctgcaa
    27481 cacagtttgc aacaagggaa atataaccag aagcgcgcag ctgaattgct ggggttaacc
    27541 tatcatcagt tccgcgcgtt gttgaaaaag caccagattt agcgaagttg ggcaatacaa
    27601 cttgccggat gcggcgtgaa tgctttatcc ggcatacata atcaatacgc cgtttgcagg
    27661 cctgataagc gcagcgcatc agacattatt gcgttgggct attaatcgct cagcgcatcc
    27721 tgcaaaattt tcattgccgc atcgaattgc gcatccggga tggtcagcgg atacaggaaa
    27781 cgaatcacgt tgccgtatgc gccacaggtc agcagaagca gcccctgcgc cagcgcgcgt
    27841 tgctggattt tctgtgcaat cgccgctgac ggctcgcccg tttgcggatc gttaaactct
    27901 gccgcaatca tagaccccag accgcgtacc gccgcgatgg ccggaacgct ttctttggca
    27961 tcaatcagcg tgtttgtcag acgctggccc agttgattcg cgcgttcgca gagtgattct
    28021 ttgtcgataa tgttgagcac cgcgtgcgcg gcagccaccg ccagcgggtt accggcgtag
    28081 gtgccgccaa gcccgcccgg cgcgggtgcg tccataatat tcgcgttacc gaccacgccc
    28141 gaaagcggca tcccgcccgc gaggcttttc gccatcgtca ttaagtccgg cttatcggcg
    28201 taatgatcca tcgcaaacag tttgccggta cgcgcaaagc cgctttgcac ttcatcggca
    28261 atcatcacaa taccgtgttc gtcgcacagg cggcgaatgg cggcaaccaa ttcttttggc
    28321 gccacgttga aaccgccctc gccctgaacc ggttcaaaaa taatcgccgc gacctgtttc
    28381 gcttcgatgt ctgatttaaa caaacgttcg atggcgtcca gggagtcctg cgttgaaacg
    28441 tcatgtaaat ctgacggata cggcacgtga tacaccgaac cggggaacgg gccgaagccg
    28501 attttgtacg gcgcgacctt tccggtcagc gccatggtca tgtacgtacg accgtggaag
    28561 ccgccgctaa acgcaatcac acctgggcgt ccggtatggg cgcgggcgat tttcaccgcg
    28621 ttctcaaccg cttccgcacc ggtggtgaag aacgcggttt tagcctgccc gcttaccggg
    28681 gcaagggcgt tgattttctc cgccagggtg acgtagcttt catacggcac aatctgatac
    28741 gcggtgtggg taaactgttg cagttgctgc tccaccgccg cgaccagatc agggtggcga
    28801 tgtccggtat tcagcaccgc aatgcctgcg gcgaaatcga tgtactcgtt gccctcaaca
    28861 tccttcagcg tagcgttttc agccgactgg gcgaagaagt tacacatcac gccaaccccg
    28921 cgcggggtgg cagaaagacg acgctgatgg aattcattgt tgctcatgtt tcacaccttt
    28981 tgactcttca ctgttaatcg gaaattaaac gcccagacga tcgcgcaggc tgtaataagc
    29041 cgcgcccatc gcggtaaacg gcacacgcag cgtgcgcccg ccggggaacg ggtaatgcgg
    29101 cagattggcg aaggcatcga aacgttcggc gtcgccgcgc agcagttcgg caatcaaacg
    29161 tccggcaaga tgagtacagg tgacgccgtg gccgctgtaa ccctgcatgt aatagatgtt
    29221 ggtatcgagg cgaccgaact gcggcatacg cgacagggtc agcaggaagt tgcccgtcca
    29281 gcgatagtcg attttcacgc ccttcaactg cgggaaggtt ttcagcagtt tcggcaccac
    29341 aagacgctca acgtcatccg ggtcgcgcgc gccgtacacc acgccgccgc cgtacagcaa
    29401 acggttgtcg gcggtaagac ggtagtaatc aagcagatag ttacagtctt ccacacagta
    29461 gtttttcggg atcagcgaac gggctaaatc ttctgacagc cgctcagtgg tgatcacctg
    29521 cgtgccgcac ggcatactac gcttcgccag ttccggctcc actttatcgc ccaggtacgc
    29581 attcccggcg acaatcacat acttcgccgt cacctggcct ttggtagtac gcaccacggc
    29641 gggcgtggtg tgctggatct gcgtcaccgc agaaagctca tacacgcgcc cgccgttcag
    29701 gcggatagcg tccgcttcac caatggcgag gttcagcggg tgaatatgcc cgccgctgtg
    29761 atccagcagc gcaccagtgt agcgatcgct ggcgacctcg cggcgaatgg cgttggcgtc
    29821 cagcagctcc agctgtttat tgccgtagcg ttcccagttc tctttctgct cttcgagtgt
    29881 ggcgagctgt ttatcattca tcgccacaaa caggccgccg gggcggtagt cgcagtcaat
    29941 ctgatagcgt ttgatacgtt cgcggatgat ctcgccaccc tcgaacatca tgctgccgag
    30001 catacgggcg gtgtccatgc cgtagctttt ttcgatcacg tcgatgtcgc ggctgtagga
    30061 gttcacaagc tgccccccgt tgcgtccgct ggcgccaaag ccgatgcgtg aggcttcgag
    30121 aaccactacg tcaaagcccg cttccgccag atgtagcgcg gaagacagcc cggtatagcc
    30181 gccgccgacc acgcatacgt cgcaggtgat tgactcattc agtgtgtcga atggcgcgta
    30241 tttattcgca ctggcggcgt agtaactgct ggtatgttcg gtcatttcag gcctccaggc
    30301 ttatccagat ggttttcagt tcggtgaatt tttcaagggc atgcagggat ttgtcgcgac
    30361 cgttgccgct ctgcttatag ccgccaaacg gcacggtcat atcgccgtcg ttgtagttat
    30421 tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg gtgcgcgcgg gagaggtcgc
    30481 gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt ggcaagctgt agcgcctgtt
    30541 cttctgatgt gaaacgcgtg accaccagca ccggaccgaa aatctcttcg cggcttaagg
    30601 acgcattcgg gtctaaatcc acaaagatgg tcgggccgat ggcggcagcc agcccggcgt
    30661 tacggccatc caacaacagt tgccctttgc tttcgccttc ccgaataaag ctatggaccg
    30721 agtcggcgtg ggcgcagtcg attaaggtgc ccatggtggt tgcgggatca agtggatggc
    30781 ccggctgcca gttttgcgcc tgctgtttta acagggctaa gaattcatcg gcgatgctct
    30841 cttccagcaa caggcgcgtt ccggcgatac acacctgccc ctggttgtag aaaatgcccg
    30901 cagcggtcgc actcgccgct ttttgcagat ccgggcagtc agcgaaaacg atgttggcgc
    30961 ttttgccacc tgcttccagc cagacgcgtt tcatgttgct gtcgcccgca tctttcagca
    31021 gctgtttccc ggtacgggac gaaccgggaa aggcgatggc atcgatatcg ttatggcgcg
    31081 acagcgcctg ccccgcttca tgaccaaaac ccgttactac gttcaacaca ccatccggca
    31141 agcctgcttc tttcgccagc cccgcgagac gaatcgcact gagcggtgat ttttcagacg
    31201 gttttagaat cacgctgttt cccgccgcca gcgccgggcc aagcttccag caagtcagca
    31261 gcagcgggaa gttccacggt acaatggcgg caattacgcc gaccggttca cgcacgatca
    31321 tcgccagctc atggctactg gtggtcgcca cttcgccata cactttgtcg atcgcttcgg
    31381 cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc tcgcagactg tgacgaatcg
    31441 gtttgccggt gtcgagagtt tccagcagtg ccagctcttc ggcgttggct tccattaaat
    31501 cggcgagttt attcagcacc gctttacgtt tagccggaga agagagtgac cagtcgccgc
    31561 gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat atcgacgctc ttgccgcggg
    31621 caattttcgc cagcggtgcc tgggtgaccg gatcaacggt ttcaaaggtt tcattttccg
    31681 ccgcagcagt atattcaccg ttaataaata agcgggtttc aatggcgaga cttaacgctt
    31741 tatcctgcca gtaagccaga tgatgaaaat tcattatgac tcctgtttca cgactatcag
    31801 atatatgcag tttgtgaacg tcagggcgca gagatccccg ggccgtatgc ccgtttattt
    31861 ctggcgacat tctttatgga ttgccgggcg cattgcgctt acccggctta cagaacaaaa
    31921 agattaaaac gtggtgggcg tatgggcgct aataattcgg caaatacctg ccgatgtatt
    31981 actgaaactg tgcgggatgc cggtattaat ggcataactt tgccccgcga cgagatggta
    32041 atcctgacca ttaatcgtca gaacaatttc accttccagt acagtgccta tttcctcacc
    32101 ctgatgctta attctttccc cagtggttgt gcccggctgg tacgtttcaa agatcatcgc
    32161 cagcgtgcga ttcgggttac cgttatgaac cagcttcatt gacacaccct gactgcccat
    32221 ctcaattaaa tcgtcctgat taatgacgac ctgcggctca tcaggttttt ccggctcgga
    32281 aaagaattcc gagagtgaca gaccatacac cttcagcagc ttttgcagcg tactgatggc
    32341 agggctgact ttatcttgtt ctatcgtact gatagcactg tgagtcagcc cggagagttc
    32401 ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt tccgacaagc gttttcctgg
    32461 cgccagtccc tcatcactca tatttgcatt tccttaaact gtagtggtca gagtcgctgt
    32521 ttttcagcga tatggtgctg acaagcggtg ataaagccct cgaacaatat acgcgaaagc
    32581 gcgtactcgc tactgttcca ttccgggtgc cactgtacgc ccagcgcaaa aggatgattg
    32641 atgacgctaa ccgcctcaac taagccatcc ggcgagcgag cttcaacacg caaccgtggg
    32701 ctaacgacct tcgccccttg tccatgcaga gagtttaccc aaaagttgct acattcaggt
    32761 aacaacgcag acagtaatcc cccctcttca acctgaactt catgcgacgg tgcatattgc
    32821 tgttccaccg gcagttcggg atcttcccga tgttccagca attcaggctg ttcgcacagc
    32881 ttgcgatgca acgaaccacc ggttgccacc accagttcct gtaaaccccg gcagatggcg
    32941 aaaatgggga tgcgcctttc gagtgcggca ttaattaacg ccatgctcag aagatcacgc
    33001 ccgggatcgg cgtcaggctc atcgccgttt tcaccatata ggtgcggctg cacattgctg
    33061 ggactaccag gaagataaat gccatcgagt ttcggcaaaa gttgttcaag taaggacggt
    33121 tccgccagcg catgtggcag cgcaattggc aagccgcctg catggatgat ggcattcagg
    33181 tacttttctt gcagagtctg ggtcgcatga cccttaagcc tgttcctgca cattacgaca
    33241 ccgataaccg gattgttcat tatattttcc atgctcaatc tcacaaagtg gactaaatta
    33301 tcgccattac tgcggtttaa atgaccttta tgttcaatat tttttcaatc tagcagtggg
    33361 ttttcatttt tgcaaaccca atttaacatt tgacaaacat ttagtttgca tacagattcg
    33421 aatggtggtc attatatttt acgctttgat aacgagcgga aaacaaacca aaggcgaaga
    33481 atcatggaaa ccaatatcgt tgaagtagag aactttgttc agcagtcaga agagaggcgg
    33541 ggtagcgcct ttacgcagga agtgaaacgc tacctggagc gctacccgaa tacgcaatat
    33601 gttgatgttt tacttaccga tttgaatggt tgcttccgtg gaaaacgtat tcctgtttca
    33661 agcctgaaga agctcgagaa agggtgttat ttcccggcct cggtatttgc aatggatatt
    33721 ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa tgggcgagcc ggatcgtacc
    33781 tgtgttcctg ttctcggttc cttaacccct tctgccgccg atccagagtt tatcggtcag
    33841 atgctcctga ccatggtcga tgaagatggc gctccctttg acgttgagcc gcggaacgtt
    33901 cttaaccgcc tgtggcagca gctgcgccag cgcggattgt tcccggtcgt agcggtagag
    33961 ctggagttct atttactgga tcgccagcgc gacgctgaag ggtatctgca accgccctgc
    34021 gcgcccggca ctgatgaccg caatacacaa agccaggttt actccgttga taacctcaac
    34081 cacttcgccg acgtgctcaa tgatattgat gaactggcgc agttacagct gattccggca
    34141 gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa tcaaccttta ccatactgat
    34201 aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac gcctggtgcg tctgatggca
    34261 gaaaagcata agatgcacgc cacttttatg gcgaagccgt atgaagagca cgcgggcagc
    34321 ggaatgcata tccatatcag tatgcaaaat aatcgtggcg agaacgtgct ttctgacaca
    34381 gaaggcgaag attcgccgct gctgaaaaag atgctcgccg gaatgatcga tctgatgccg
    34441 tcatcaatgg cgttgttggc accaaacgtg aactcgtatc gccgcttcca gccggggatg
    34501 tatgtaccga cgcaggcgtc gtggggccat aataaccgta ccgtcgccct gcgtattcca
    34561 tgcggcgatc gtcataatca ccgcgtggaa tatcgcgtgg cgggtgccga tgccaaccca
    34621 tatctggtga tggcagcgat ttttgccggt attttgcacg gtcttgataa cgagctgccg
    34681 ttgcaggaag aagtcgaagg caacgggctg gaacaggatg gcttaccctt cccgattcgc
    34741 cagagcgatg ccctgggtga gtttatcgag aatgatcacc tgcgccgcta tttaggcgaa
    34801 cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc tgttgcagtt tgagcgcctc
    34861 atcacagaaa cagaaattga gtggatgttg aaaaacgcat agtacccctt gcggggttgt
    34921 ttcatcggcc tgagcggagc caggcctgac atttgctggt aatccagaca tttcccggat
    34981 gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa cgattaagac tgtgacgagg
    35041 aaatgagatg atgccgatga ttaaatcccc gcatggcgag ggaggctgcg tatgcgcccc
    35101 tcctgcgact gactggactc cgcccccttt gcttcccttg ctaaacaggt ttgatttcag
    35161 gtcgacacga ccgcaaacct tattacgcag gggaggcagc aattatggct attaattcac
    35221 cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg aaaatcactg aaattgtggc
    35281 aggtggtgat gatgggtctg gcctatctca cgccgatgac tgtgttcgat acctttggca
    35341 ttgtatctgg cattagcgac ggtcacgtcc cggcatccta tttgctggcg ctggcgggcg
    35401 tgctgtttac cgctatcagc tacggcaaac tggttcgcca gtttccggag gccggttcgg
    35461 cctataccta cgcgcaaaag tcgattaatc cgcacgtcgg atttatggtc ggctggtcat
    35521 cactgctgga ttatctcttt ttgccgatga tcaacgtact gttggcaaaa atctatctct
    35581 ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt gaccttcgtc gccattttaa
    35641 ctgccgcgaa cctgaagagc gttaacctgg tcgccaactt caataccctg tttgtgctgg
    35701 tgcaaatctc catcatggtg gtgtttatct tcctggtggt tcagggactg cataaaggag
    35761 aaggcgttgg caccgtctgg tcacttcaac cgtttatcag cgagaacgcg cacctgatcc
    35821 cgattattac cggggcgacg attgtgtgct tctcgttcct cggtttcgat gcggtgacca
    35881 cgctttcgga agagacgccg gacgccgcac gcgtgatccc gaaagccatc ttcctgacgg
    35941 cggtctacgg tggcgttatc tttatcgcgg cgtcgttctt tatgcagctg ttctttcccg
    36001 atatcagccg ctttaaagac ccggacgctg cactgcctga aattgcgctg tacgtcggcg
    36061 gcaagctgtt ccagtctatt ttcctctgca ccacatttgt gaacacattg gcctctggcc
    36121 tggcttcaca tgccagcgtg tcgcgcctgc tgtatgtaat ggggcgtgac aatgtgttcc
    36181 cagaacgtgt gtttggctat gtacacccga aatggcgtac cccggctctg aacgtcatta
    36241 tggtcggtat tgtcgcgatg tcggcattgt tctttgattt agtcaccgcg acagcattga
    36301 ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct gtcggtgttt aatcatttct
    36361 ggcggcgtaa aggaatgaat aaaagctgga aggatcactt ccactatttg ctgatgccgc
    36421 tggttggcgc gctgacggtg ggtgtgctgt gggttaacct cgagtcaacg tcactgacac
    36481 tcggtctggt atgggcttcg ttaggcggcg catatttgtg gtatctgatc cgccgctatc
    36541 gcaaagtgcc gttgtatgat ggtgacagaa cgccagtgag cgaaacgtaa tagtcggatg
    36601 cgcggcgcat tatcctgcct gagggtaatg cgccgcgcca ttaactataa aaagatgtat
    36661 gacccggatc acgagtgcta tcatccgccc ttatgcttag cgcattaacc ccaggggcaa
    36721 ataatgaacc acgatatccc gctaaaatat tttgatattg ccgatgagta cgcgaccgaa
    36781 tgcgcagagc ctgtcgcaga cgctgaacgc acaccgctgg cccactactt ccagttactg
    36841 ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag cccaacatga aatggctgct
    36901 gaagcgggga ttaatcccgt acgcattgat gagatcgcag agtttttgaa tcaatggggt
    36961 aatgagtaat tatttaattg cagacgatcg gtcagtatcg ttgtctggat aaagcgttca
    37021 cgccgcatcc ggctaacaat gacacgcact gcagcgtgct ttaatgggtt tctcacgatc
    37081 gtaccgctct gctcctcgct aagttgttct gtgagttata gttatagaga aatcacgggt
    37141 acgaatgttg aacagatggc gacgccacct gttcaacgac caaccacacg gccctcatta
    37201 cgacccctga acacaaacgg taatggcaaa tgtcattaaa tattagtaag atagcttctt
    37261 tacataaaat cccttaatat tatgcgccag gtattatcgt ctcttttggt gattgctgga
    37321 cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc cgcatgctga tatccgcgac
    37381 agcggttttg tctattgcgt cagcgggcaa gtcaacacct ttaacccatc caaagcgagc
    37441 agtgggttaa ttgtcgatac ccttgccgcc cagttttatg atcgactgct ggatgttgat
    37501 ccctatacct atcgcctgat gccagaactt gccgaaagct gggaagtact cgacaacggc
    37561 gcgacctatc gcttccacct acgtcgcgat gttccgtttc aaaaaaccga ctggtttact
    37621 cccactcgta aaatgaatgc cgacgatgtg gtgtttacct tccagcgaat ttttgaccgc
    37681 aacaacccgt ggcataacgt caacggcagc aacttcccct acttcgacag cctgcaattt
    37741 gccgataacg tgaaaagcgt ccgtaaactg gataatcata ccgttgagtt ccgtctggct
    37801 cagccggatg cttctttttt gtggcacctc gcaacccatt atgcttcggt catgtcggca
    37861 gaatatgccc ggaagttaga gaaagaagat cgccaggagc aactcgaccg tcaaccggtc
    37921 ggcactggac cgtatcagtt gtcggaatac cgcgccgggc aatttattcg cctacaacgt
    37981 catgatgact tctggcgcgg taaaccgtta atgccgcagg tggtggtgga tttaggctcc
    38041 ggcggcaccg gacgtctgtc gaaactcctg accggggaat gcgacgttct ggcctggcct
    38101 gctgccagcc agctatccat tttgcgtgac gacccgcgct tgcgtttaac gctgcgtcct
    38161 gggatgaacg tcgcctatct ggcatttaac accgccaaac cgccgctaaa taatcccgct
    38221 gtccgccatg cgctggcact ggcgattaat aaccagcgcc tgatgcaatc catctattat
    38281 ggtacggctg aaacggcggc ctctatttta ccgcgcgcct cgtgggccta tgacaacgag
    38341 gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac agttgaagtc gttggggctg
    38401 gaaaatttaa cgctgaaact gtgggtgccc acacgttcgc aggcgtggaa ccccagtcca
    38461 ctgaaaactg ccgaactgat tcaggcggat atggcgcagg ttggcgtaaa agtggtgatt
    38521 gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg atatgagcca tgatctgacg
    38581 ttatccggtt gggcgacgga cagtaacgac ccggacagtt tcttccgtcc gttactgagc
    38641 tgcgcggcaa ttcattcaca gaccaacctc gcccactggt gcgatccgaa attcgacagc
    38701 gtgttgcgta aggcgctctc ctcgcagcag ctggcggcgc gtattgaagc ctatgacgaa
    38761 gcgcagagta ttctggcgca ggaattgccc attttgccgc tggcgtcgtc attgcgtttg
    38821 caggcctatc ggtacgatat caaaggtctg gtacttagcc cgtttggtaa cgcctccttt
    38881 gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac catgattatc ttcaccttac
    38941 gccgcatttt gttattgatt gtcaccttgt tcctgctgac cttcgttggc ttcagcttga
    39001 gctatttcac tccacacgcg ccgttacaag gcgcgtcact gtggaatgcc tgggtgttct
    39061 ggtttaacgg cctgatccac tgggattttg gtgtttccag cattaacggt caaccaattg
    39121 ccgaacagtt aaaagaggtc ttcccggcga cgatggaact gtgcatcctc gccttcggtt
    39181 ttgcgctgat tgttggcatc cccgtgggga tgattgccgg gattacgcgc cataagtggc
    39241 aggataactt gatcaacgcc atcgccttac tgggcttttc aatcccggtg ttctggctgg
    39301 cgcttctgtt gacgctgttt tgttcactca cgctaggttg gttgcccgtt tcagggcgtt
    39361 tcgatctgct ctacgaagtg aaaccgatta ccggttttgc gttgattgat gcctggcttt
    39421 cggactcacc atggcgggat gaaatgatca tgagcgcaat ccgccatatg atattgcccg
    39481 tgatcaccct gtcggttgcg ccaacaactg aagtgatccg cctgatgcgt atcagcacta
    39541 ttgaagtgta tgaccagaac tatgttaaag cggcggcgac ccgcggtttg tcgcgcttta
    39601 ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc ggttattcct cgcctgggct
    39661 tacagttttc caccatgctg acgctggcga tgatcaccga aatggttttt agctggccgg
    39721 gtttagggcg ctggttaatt aacgccatcc gccagcagga ttacgcagcc atttccgccg
    39781 gagtgatggt gtgtggctca ctggtcatta ttgttaacgt gatttctgat attttgggtg
    39841 ccatggctaa ccctctgaaa cataaggaat ggtatgcctt acgatagcgt atacagcgaa
    39901 aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat tttatagtga cgcctctgcg
    39961 atggtcggcc tgtacggctg cgcggggctg gctgtactgt gtatttttgg cggctggttt
    40021 gcgccttacg gcatcgacca gcaatttctc ggttaccaat tattgccgcc gtcatggtcg
    40081 cgctatggcg aagtttcttt cttcctgggg actgacgacc tcggacgcga tgttttaagc
    40141 cgtttactga gcggagctgc gccaaccgtg ggtggcgcat ttgtggtgac gcttgccgcg
    40201 acgatctgtg gcctggtact gggcaccttc gccggggcga cacacggcct ccgctcagcg
    40261 gtgcttaacc atattctgga taccttactg gcgatccctt ccctgctgtt ggcaattatc
    40321 gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt ttgctgtctg gctggcattg
    40381 ctgccgcgta tggtacgttc gatttacagc atggtgcatg acgaactgga aaaagagtac
    40441 gttatcgccg cccgtctgga tggcgcatca acgctgaata ttctctggtt tgctgtgatg
    40501 cctaacatca ccgccgggct ggtcaccgag atcacccgcg cactgtcgat ggcaattctc
    40561 gatatcgccg cgctgggctt tctcgatctc ggcgcacaac tcccctcgcc tgaatgggga
    40621 gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc cgtggactgt catgctgcca
    40681 ggtgcggcaa ttatgattag cgtgctgttg gttaacctcc ttggcgacgg tgtccgtcgt
    40741 gcaattattg cgggggtgga ataatgccat tactcgatat tcgtaacctg actattgaat
    40801 ttaaaaccgg ggatgagtgg gttaaagccg tcgaccgggt aagtatgacc ttaaccgaag
    40861 gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa gagtttgatt gcgaaagcga
    40921 tttgtggggt gaataaagat aactggcgtg ttactgctga ccgtatgcgt tttgatgata
    40981 tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact ggttggccat aacgtgtcga
    41041 tgattttcca ggaaccgcag tcgtgtcttg acccttcaga acgtgtgggt cgccagttga
    41101 tgcaaaacat cccagcctgg acctataaag gccgttggtg gcagcgtttt ggctggcgca
    41161 aacggcgtgc cattgaactg ctgcaccgcg tggggattaa agatcacaaa gatgcgatgc
    41221 gcagttttcc ctatgagttg accgaaggtg aatgtcagaa agtgatgata gccattgcac
    41281 tggcgaatca accgcgtctg ctgattgctg acgaaccgac aaactcaatg gagccaacaa
    41341 cccaggcgca aatctttcgc ctgctgacgc gtctaaacca aaacagcaat accactattt
    41401 tgcttatcag ccatgactta caaatgctta gccaatgggc ggataaaatt aacgtgcttt
    41461 actgcggtca aacggtggaa accgcgccga gtaaggagtt agtgacgatg ccacatcatc
    41521 cttataccca ggcgctgatc cgcgcgatac cagacttcgg cagcgcgatg ccgcataaaa
    41581 gtcgcctcaa tacgctaccc ggcgctatcc cactgctgga acagttaccg attggttgtc
    41641 gtctggggcc acgttgccca tatgcacaac gagaatgcat tgtgacgcca cgtttgacgg
    41701 gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa catggagaaa gagtgagatg
    41761 atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt tccgctaccg gaccggttgg
    41821 tttcgtcgcc agaccgtaga agcggtaaaa cccttgagct ttacgctacg tgaaggccag
    41881 acactggcga ttattggcga gaatggttcg ggtaaatcca cgctggcaaa aatgctggcg
    41941 ggaatgatag aacctaccag cggcgagtta ttgattgacg atcatccact gcattttggc
    42001 gattattcct tccgcagtca gcgtattcgc atgatttttc aggatccttc gacctcgttg
    42061 aatccccgtc aacgtatctc gcaaattctg gattttccac tgcgcctgaa caccaacctg
    42121 gaaccggaac agcgtcgcaa acagattatt gaaacgatgc gtatggttgg gctgctaccg
    42181 gatcacgtca gttactatcc gcatatgttg gcccccgggc aaaaacagcg tctgggtctg
    42241 gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg atgaagccct cgcttcactg
    42301 gatatgtcga tgcgttcgca gttgattaat ctgatgctgg aattacagga aaaacagggc
    42361 atttcgtata tttatgttac ccagcatatc ggaatgatga agcacattag cgaccaggtg
    42421 ctggtgatgc atcagggcga ggttgtcgag cgaggcagca ccgcggatgt gctggcatca
    42481 ccgctgcatg aactcaccaa acggctgatt gccggtcatt ttggcgaggc attgacggcg
    42541 gatgcgtggc gtaaggaccg ttagtgttct gcacctcatc ctgacagggg ccccccctca
    42601 ggatgaggta ttcattactt ttgattgtcg attctggtga gagcaaccag gaatgccagt
    42661 gtataacaca ccgtaattgc cagactcatt tttaacagtg tttctccgcc aaatcccgac
    42721 aatggcgcgg ctataccgcc gaagacaaac atgctcatcc ccatcaacgc agacgccgtt
    42781 ccagattcct gtgtccctac tgcactcata gcctctgccc cgccaacagt gccgatgccg
    42841 ctacaaaatg caatagtgaa aaataatgcc accagtgccg gaatgggcaa ttgtgtccat
    42901 gcgcataaga ccgtgagcaa tgcacacaaa attgccgcta tcaggccacc tcgcaacaat
    42961 gtcatcgcat taatccgtcg cgccagacgc gaaaagatcc atgaagcaat gataagtccg
    43021 atgccgttaa ggccaaacac caggctaaat tgcattggac taaagccaaa ttccttctgc
    43081 aacacgaacg acgaagagcc gatatacgca aacaagcctg ccagcataaa actttgaatc
    43141 aggcaaaagc gcataaaacg gcggttctgc accacgcttc gtccggttaa tagcaatgat
    43201 gagccccttt tattttctgg caaggtctca ttaataaata acacgcagcc cagcaacaat
    43261 acggtgctaa tttcagccat tacccagaat aaagtgcgcc agtcaaaagt gctaacaatg
    43321 tacccgccca gcactggcga caacaccggt gccaggccat tcaccgtcat taacagcgca
    43381 aaaaactggg tcaacgttac tccctgatat ttgtcacgag caatagaacg agagagcacc
    43441 gaaccacccg cccccgcgat cccttgaata aaacgccaga ccaccagcca gtaaatattg
    43501 ttcgttgtcg cgcacaaaat ggaagaaaca ataaacaata gcaacgacaa gattaacgga
    43561 cgtttacgcc ctattttgtc gctcagaggg ccaaataaca gttgtccgac gccaagaccg
    43621 atcagtgatg cagtcagcgt taattgcgtt atggtcgtgg ttgccgccag ttgctcagac
    43681 atctccggca aagccggcaa ataaagatcg gtacacatcg ggccgatacc ggctaaaaga
    43741 ccaagaatca atgcccatga aggagagact ctagccatat tattccttac ttgagtgatg
    43801 acatgccgcc gcccagcgac tgccagagcg tgattcggtt tgtaaaatca gtcagttgca
    43861 gtgccagcag ctcctgctgt gcgctccaca agctgcgctg cgccgtcaac acggtcaggt
    43921 aatcaccaac acccgtctga tagcgacgca agccaacatc gaccgtttgt tgctcagctt
    43981 taacatactg gcgctgtgca tccagttgct cttcaagcgt ggtccgccgc gccagtgcgt
    44041 tagcgacatc tttaaatgcg ctctgaacgg ttttttcata ggtggcaatg agtccacgct
    44101 tttgtgcttc tgcgtaacga agctgcgcca gattacttcc gccagtgaac agcggtaatg
    44161 tgaccgatgg cgtaaatgac cagatctgca ttccgtggct gaacaacgac gacaatgcgt
    44221 cactgccaac accagcgctg gcagtcagcg aaatcgtcgg gaaaaagtta gcccgcgccg
    44281 cgccaatatc ggcattggcg ctcttcaaat tatgttccgc ttcctggata tccgggcgac
    44341 gcagcagaac gtcagatgac actcccgcag gaaccagact tatcatctgt tccggcaggc
    44401 tttccagtgt tcccggtagc aaattttcct caagcgttgt acctgccagt aagttcagcg
    44461 catttttatc ctgcatcact tgtgtctgat agctggcaac gctggcgcgc gcttgctgat
    44521 aaacactcat cgcctcactg acatctgtcg ctgccgccgt gccaacctgt tgctggcgct
    44581 ggataatttt caatgagttt tctgcactgg tcatcgtttc ttttgccagg gccaggttgc
    44641 tgttatcggc cgccagtgtc agccaggcgg tgctgatttc agcaatcagc gttaaacggg
    44701 tgttctgggc tgtaaattca ctggcaagcc aggtttctcg cgccgcccga gataaactct
    44761 gattacgacc aaacagatct aacgtaaaac tggacaccgt gccgtcagcc tcggcagtgg
    44821 tttccgtacc attcgccagc gaacgactgc gggttgaact taaggccgca ttcaccgtcg
    44881 ggaataacga cgcattagtt tgcccataga gcgcccgtgc tgagtcaata tcagcaatcg
    44941 ctttttgcac atcgcggtta ctgttcagtg caatagtcac cacctgctgc aatcggggat
    45001 cgtggatcac ctgctgccag tcatggctga ttgcctttcc ctggccctgt gctcccggca
    45061 aggtcgccgg gattggggat tcaggcgtgc tgtagtgggg atcgagcgag acacatcccg
    45121 cactcaacag caccagaaaa ataagagaac gacgcaacat aacttactcc tgacggcgcg
    45181 atttaccggc aaacaaacgt ttcaccagta caaaaaacag aggaacaaag aaaatagcca
    45241 gcaacgtagc ggtcagcgta ccgccaataa tgcccgtacc aatggcgatg cggctgttcg
    45301 cccctgcgcc ggttgctatc gccagcggca ttacccccgc aataaacgcc agcgaggtca
    45361 tgatgattgg gcgtaaacga gtctgcgccg cgcgtaatgc cgcacgactc aaggaataac
    45421 cctctgcaac cgccgcttca gcaaattcca caatcagaat ggcgtttttc gacgacaagc
    45481 cgatagtggt taatagcgcc acctggaagt aaacatcgtt gtttaaatca cgcatccagg
    45541 ccgccagcgc cgcgccaaga agccccagag ggatcaccag aatgaccgag aacgggactg
    45601 accagctttc gtacagtgct gcaaggcaga ggaaaaccac cagaatagaa actgcataca
    45661 gactcaatgc ctgaccgctg gccagtttct cctgcaatga caaaccactc caggcccagg
    45721 ttgttcccgc aggaagactg tttgccagtt cctccatttt tgtcattgca tcgccggaac
    45781 taaagccagt cgcattttcc ccctgaattt cataggctgc agaaccgttg tagcgcacca
    45841 gtctttccgg tccatacagc cagcgggtgg tcgcaaaagc agagaatggt gtcatggcgt
    45901 tatcactacc gcgcacaaac catttaccta agtctgacgg cgcggaacga aattcactgt
    45961 cgccctgaat gtagactttt ttcacacgac cacggtcaat aaagtcattc acataagtac
    46021 cgccccacgc actggacagg gtgtcggtga catcattcaa actcaaccct aatgacaccg
    46081 ctttattact atcaatatct acctgcaatt gcggcatttg tggcaaatca ttggcacgaa
    46141 cagaatgtaa ctccggactt tgattcgctt tttctatcaa ctgattacgc atttgcagta
    46201 gtgtttcgcg atcggttcca ccgttagcta acaattcaaa cgtaaaacca ttgctttgcc
    46261 ccagcccatc aacggctggc ggcgtcatcg caaataccgt ggcatcacga attgtgccca
    46321 gctctttggt tgcccgtagg gcgatagcct gggcggtgtt ttctgcccct ttacgttgag
    46381 accagttttt caacgaaaca aacgccatcc cggtgttctg tccgctgccg ctaaaactga
    46441 aaccatcaac ggtaaaaatg acatcggtat ttgctttctc gttaatcagg aaccagtcaa
    46501 caatctggcg attcacttct gctgtacggg cagcggtcgc cccggccggc agcgtgtact
    46561 gcaccatgat ttcgccctgg tcttcagtgg gtaagaaact gcccggcagt ttccacatca
    46621 tcagggccat tccaccaccc agtacgagat aaagccccat cgttcgagca gcacggcgca
    46681 ggacataaat tacgcctcgc tgatatttat cttcagtacg gcggtaaaag cggttaaatg
    46741 cgccgaaaaa gcctttttta tgtggcggaa catgctggag gacggaaccg cacagggcgg
    46801 gagtcaaggt caatgccacc accacggaaa gcagcattgc ggagataatg gttatcgaaa
    46861 actgacgata aattaccccc gtagatcccc caaagaaggc cataggcagg aatacggctg
    46921 acaacaccag cgcaatggca accagtgcgc cagaaatctc gcccattgat ttttccgtgg
    46981 cttcacgcgc gggcaaccct tcgtcacgca taatgcgttc gacgttctcc actaccacaa
    47041 tggcatcgtc caccagcaag ccgatcgcca gcaccattgc aaacaacgtc agggtattaa
    47101 tcgaatagcc aaacaacgca agaacgccga atgtgcccag caaaacgacg gggacagcta
    47161 acgccggaat cagtgtggca cgcaggtttt gcaggaataa atacatcacg caaaccacca
    47221 atacgatggc ttcaaacagc gtctgaatta cgtcctctac agagatttta ataaattcag
    47281 tgctgtcttt cgggtaagcg atgtcgtatc cctgcggcat gttccgctgg aattcggcaa
    47341 tcttatcctt gaccagcgtc gccgtattca gcgcattcgc accgggcgaa agcattacag
    47401 caaccccggc agacgggtga ccgttaagtt tgccgatagc ggtataatct tcactgccca
    47461 tctcaacgcg agccacatct tttatacgaa caactgcgcc gtctgactgg cttttcacga
    47521 taatattttt gaactgatcc accgtctgca agcgagactg cgcacgtacc gttgcggtca
    47581 gttgctgagt attcggtgaa ggcaatgccc ctattttccc ggcagtaacc tgcacgtttt
    47641 gcgcttcaat agcactttgc acgtctgaag gcattagcga gtaagacgcc agtttggccg
    47701 ggtcaagcca gatgcgcata gcgtattccg cgccaaagac ttgcagactc cccacacctt
    47761 caacacgcgc cagcgggtcc tgaacgttac taaccaacca gtcggcgata tccgaactgg
    47821 aggctttgtc agtggtgtca tacacggcag caatcagcaa aaagttactt tgtgatttct
    47881 ccaccgtaac acccgtttgc tgcacttcgc tgggtaggcg cgactccgcc tgctgaattt
    47941 tattctgcac ctgaacctgt gcagtatctg gatcggtacc ttgttcaaag gtcacattaa
    48001 tactgaccga accatcagag ctgctggttg agctgaaata gagtaaatta tcaagcccag
    48061 tgagttgttg ctcgataacc tgagtcacgc tgttctccag cgtttcggca gaagcaccag
    48121 tataagtggc tgaaatttta atggtcggtg gcgcaacgtc aggatattgc gcgacgggca
    48181 atgtgcgaat agccagaatc cccgccaaca taatcaaaat ggcgataacc caggcaaaga
    48241 ccgggcggcg aacaaaaaag cgcgaaaaca tcaggcgttg cctccattag cctgaacttc
    48301 aacagccttg accgtctgcc ctgaagtgac ttttgcagaa ccttcaacaa tcagtcggtc
    48361 gccgttgtgc aggccgttta gcaccagcca tttatcacca tatgtttctc ccgtttcgag
    48421 cgttcgctgc tctactttat tgtctttatt caccaccagc gcagttgcat tgcctttagc
    48481 atcgcgcgtg acgccctgtt gcggcgcgag aatagcgtct tccatcacgc cttcatcgac
    48541 acgagcgcga acaaacattc ccggtaataa ctgctgttgt ggattgggga aaatcgcccg
    48601 taatgtcacc gaaccggtag actcatctac agcgacttcg gtgagttcca gacgcccttt
    48661 ttcgctgtag gttgtgccat cttccagaat aagtgagacg ctcatggtgt cactgttggt
    48721 cgccagtgac tgtttacgca gacgtaataa atcgacactg gagcgagtga ggtcgacata
    48781 cattgtatcc agaccacgaa tagtcgttaa cgctgtatct tgcgacgcgg tcaccagtgc
    48841 gccaggggtt accgacgaaa tgccaatgcg ccctgaaatc ggtgcggtta ccgtggtcca
    48901 gtcaagatta atgcgcgcag tttccagtgc ggcttttttc gcctctacac tggctttatc
    48961 ttgtgcacag gtagactgag catcatcagc atcctgttgt gaaacaccgt tctctttcac
    49021 cagtcgggca taacgctgcg ctttctggca atcggctttt accagtgcct gtgcttgttg
    49081 taatgctgcc cgagcttcat tccatgcagc ctgataactg gccgcatcaa tctggtagag
    49141 cggctgtcca gccttgacca gatcaccttc cttaaataag cgtttctgga taattccccc
    49201 aacctgcgga cgtacttcgg cactgaacgc agcactggtg cgtccggtta attcagtgac
    49261 taccgagacc ggttgagatt tgactgtaac aacgccgact tctgtttctg acggggatga
    49321 attgttactt tgcgtattat cacaaccaga taagagcagc attgccacta cagatgttgc
    49381 tatatacttc attattttct ccagggtgag aaataccctc ctcattaaaa ttaatgagga
    49441 tatgtcaggg atattcagtt ataacgaacc cgcatcgcga aatgaatttc gcaatgaaat
    49501 aagggggatt aatatccccc gctattggtt aattctgttc ttatttattt atcaacatac
    49561 taacaatatc ctgatatatt tcctgcagat gttgtggatc gcttttttgc ggtgttaaac
    49621 gacgataaac cgtaccttcc atcatggtag caaacacttc aacgcagcaa cgaatgtgct
    49681 cgtcgctaag atggggatgc attcttttca tatgtgcaca accgttagcg aacattctgg
    49741 cgtcagcttc ttcaagcatt gccataaccc gcgggttgcg tgtggcttct gcagccactt
    49801 ccagcatcag cgcgtcgtcg tcctcattta aggatttacg caaggcaagg acttccggga
    49861 agtgatcggt acgcgcgtca atgtccattt gggcaatacg aaagtcgatg atacggcgaa
    49921 ccatctcttc aataatggcg tctttgttgg cgaagtaacg atatatctga cccacgctaa
    49981 gctgagcttc gctggcaatt tgcgacatac tggcggcatg aaacccgcag cgacgaaaac
    50041 accgacgggc ggcgttaatg atttcatcct gacgctgttt gtggcgtatc tccagcttgc
    50101 tggtcatttc tgtctcctgg cctatcactt ggtgagaatg atcgttctca tttttgctga
    50161 ttatacgttt ctccaaagta atggaaagta tttttatctt attcatggtg catattctga
    50221 taatgaagag attgtggaga ttctgtgaag atagttgaca atattaagtt ggggtcaatt
    50281 catcagaaag gctgctactt gttgtcgcca gttctgtttt catcatcaaa cttatcggag
    50341 ctaatacgaa tagtttcata gtcagattct ataagatatt ttcccaacct accccttgca
    50401 ggcgtcaaga gtagataacg caaaatgctc tgtaccgcag cttctctccg gtacagaaag
    50461 cgcaactata gccacccaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    50521 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    50581 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactgtc
    50641 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    50701 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttccaatat
    50761 cgttctgcaa tgcgatgtgg ccgaagatgc cagcatcgac accatgttcg ctgaactggg
    50821 gaaagtttgg ccgaagtttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    50881 gctggatggt gactatgtta acgccgttac tcgtgaaggc ttcaaaattg cccacgacat
    50941 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    51001 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    51061 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    51121 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcctccgg
    51181 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    51241 cgttactatt gaagatgtgg gtaactctgc ggcattcctg tgctccgatc tctctgccgg
    51301 tatctccggt gaagtggtcc acgttgacgg cggtttcagc attgctgcaa tgaacgaact
    51361 cgaactgaaa taatcgttct gttggtaaag atgggcggcg ttctgccgcc cgttatctct
    51421 gttatacctt tctgatattt gttatcgccg atccgtcttt ctccccttcc cgccttgcgt
    51481 caggataacg atttccttta cgaccaagga gcgcccatgg aacaacgcca catcaccggc
    51541 aaaagccact ggtatcatga aacgcaatcc agtactacgg agtatgacgt tctgcctctg
    51601 gtcccggaag ccgcaaaggt cagcgatccc tttctactcg acgtgatcct tgaaaaagaa
    51661 acgctggccc ccttcctttc atggctggac cctgcgcgtg ttcttgcagt ggagttgttc
    51721 cctgaccagc ttaccgtgac ccgttcacag accttcaccg cttatgaacg cttgtcgacg
    51781 gccctgacgg ttgctcaggt ttgcggcgtc cagcggttat gtaactacta ttcggcgcga
    51841 cttacgccgc tccccgggcc tgattccacc agggaaagta atcatcggtt ggcacaaatc
    51901 acgcaatatg cccgccaact ggctagctcg ccttctatta tcgacaaccg atcgcgccag
    51961 catctgaatg acgtcggtct tactgcctgg gactgtgtga tcattaacca aatcattggt
    52021 tttattggct ttcaggcgcg gacaattgcg acatttcagg cttatctcgg gcatccggta
    52081 cgctggttac ccgggctgga gatacaaaac tacgccgacg cgtcactgtt tgctgatgaa
    52141 tcattacgct ggcgaagcag ctatgaggtg gaaaaactac ctgaagaaca cacaaaaagt
    52201 tcaactgcag aactttgcca actggccgaa atactctctc tccaccctat ttcactttcc
    52261 cttctcgaaa ggttgttaaa cagcacacgg gttaatacac agccggataa tcagcttgcg
    52321 gcgttgttat gcgcgcggat aaatggcagt cctgcttgtt ttgccgcctg tatggattca
    52381 tcaaatgaat ataaaaaaat cagcaccctt ctgcgcaagg gcgaaaatga aattaaccaa
    52441 tgggctgacc gtcattctgt tgagcgcgct accgttcagg cgatacaatg gctgacccga
    52501 gcacccgatc gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca
    52561 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    52621 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    52681 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    52741 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    52801 aaaaggcttt ggcttcctgg aagtcgacgc gcaaaaaagt tatttcattc cgccgccaca
    52861 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    52921 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    52981 tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccacttttaa aagacgccat
    53041 tccttgccgc gcagcccgtg gtctgaacca cgagtttaaa gaaggcgact gggctgttgc
    53101 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgcagaac tgacacaata
    53161 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    53221 ggaaaaagaa gcgccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    53281 agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga
    53341 cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga
    53401 tccaaccgcg tggattgctg aaggcagcaa actggacaaa gccgcgaaaa ttcgcgcatt
    53461 caccaactat ctgcctggct tcaacatccc tatgctgcct cgcgagcttt ctgacgatct
    53521 ctgctcactg cgcgccaatg aagtccgccc agtactggca tgccgcatga cgctctccgc
    53581 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    53641 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc agcctgaaag
    53701 tgaagcaatt gccgaacaag tccgtttgct ggcgcaaatt tgccaacgac gcggcgagtg
    53761 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    53821 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    53881 agaagcgatg attgccgcta acatttgtgc ggctcgcgta ctgcgcgata agctcggttt
    53941 tggtatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    54001 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    54061 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    54121 cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga
    54181 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    54241 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtcca caggatgaaa tcactgtgca
    54301 aatggccgag cgtcgccgtc tcaaccggat ggcagaacgt gatgttggtg actggttata
    54361 cgcacgcttc ctgaaagaca aagccgggac cgacacccgt ttcgcagcgg aaattgtcga
    54421 tatcagccgt ggcggcatgc gtgttcgttt ggttgataac ggcgctatcg cctttattcc
    54481 tgcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcaccgt
    54541 acaaattaaa ggtgaaacgg tttataaagt aactgacgtt attgacgtca ccattgccga
    54601 agtccgcatg gaaacccgca gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    54661 ccattcctta tgaatgggcc gtttatttcc ccgctctacc ttcatcatat cccggcagtt
    54721 ttttaactga ctttcgtttg aaaactggcg tttttccaaa actagattac aaataaatat
    54781 ataaaaaaaa caatgtgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    54841 tttaaccgcg gactccgctg ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    54901 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag
    54961 cgatgttttg cgcttttcta ccaccgaaac cacagaacct gaacgtacgt tgcagttatc
    55021 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    55081 tctgaccgtc gatgagagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg
    55141 ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcagttgctc gtgatttatc
    55201 acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    55261 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca cagggttgaa
    55321 cgaacacgac gtcattggcc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    55381 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    55441 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaat ttgtccacag
    55501 cggcagtggc aaaaacgaga tttttttaat ctgttccggc accgacatta ccgaagagcg
    55561 ccgcgctcag gagcgactgc gtattctggc aaataccgac agtatcaccg gactgccgaa
    55621 tcgtaacgca atgcaggagt taatcgatca cgctattaat catgcagata acaataaagt
    55681 tggggttgtg tatcttgatt tggataattt taaaaaggtc aacgacgcct atgggcattt
    55741 gtttggtgac cagttattac gcgacgtgtc attggctatt ttaagctgtc tcgaacatga
    55801 ccaggtgttg gcgcgtccag gtggcgatga gtttctggta ctggcatcca acacctcaca
    55861 aagcgcgctg gaagcaatgg catcacgaat tttgacccgc ttacggctcc cctttcgcat
    55921 tggtttaatt gaagtttata ccagctgttc agtaggtatt gcactctctc ccgaacatgg
    55981 ttcggacagc gcggctatta ttcgtcacgc cgacacagca atgtacacag cgaaggaagg
    56041 cggacgagga caattttgcg tttttactcc agaaatgaat cagcgagtat ttgaatatct
    56101 ctggctggat accaacttgc gtaaagcact ggaaaacgat cagttggtta ttcactatca
    56161 accgaaaatc acctggcgtg gcgaagtgcg cagtctggaa gcactagtac gttggcagtc
    56221 acctgaacgt gggttgattc caccgttgga cttcatttcc tacgcagaag agtcagggct
    56281 aattgtgcct ttaggccgtt gggtgattct cgatgtcgta cgccaggtgg cgaagtggcg
    56341 ggataaaggc attaacctgc gagtggcggt aaacatttct gcacgtcagc tcgccgatca
    56401 aaccattttc accgccctga aacaggttct ccaggaactc aattttgaat actgccctat
    56461 tgatgttgaa ttgacagaga gttgtctgat tgagaatgat gaactggcac tgtctgttat
    56521 tcaacaattt agccaactag gtgcgcaagt gcatctggat gattttggta ccggctactc
    56581 ttcactttcg caactggcgc gctttccgat cgatgccatc aaacttgacc aggtttttgt
    56641 tcgagatatt cacaaacaac ctgtctcgca gtcactggtt cgggcaatcg tcgctgtggc
    56701 ccaggcattg aatcttcagg tgatcgccga aggtgtagag agtgcaaagg aagatgcttt
    56761 tttaaccaag aacgggatca atgagcggca aggatttttg tttgccaaac cgatgcccgc
    56821 cgtcgccttc gaacgctggt ataaacgcta tctgaagcgc gcataagtag atgaatatat
    56881 atacttagcg ctatattccg tcattatata tcgtattccc ggatgataat tagccgttgc
    56941 tgttaaacac ttctctttta gcggcaattt ttgcattatt cattttcccc cttttgctat
    57001 aaggatgtcc ttgatgtctg aatttgatgc ccagcgcgtt gccgaacgta ttgatattgt
    57061 gttagatatc cttgtggcag gcgattacca ctctgcgatc cataatctcg agatacttaa
    57121 agcagaactc ttgcgccagg ttgctgaatc tacgccagac atcccgaagg caccgtggga
    57181 gatttaacaa tcgtgccttc aggttttcct atttgaggat gcccggcgcg tcagtatttt
    57241 tttatttagt attataacgt tataagaatt acagcgatga attcccgaca acaaactatt
    57301 ctacagatgg tcattgacca gggtcaggtt agcgtaaccg atctggcaaa agccactgga
    57361 gtttctgaag ttaccattcg ccaggatctc aacaccctcg aaaaactgag ttacctccgc
    57421 cgtgcacatg gctttgcagt ttcgcttgat agtgatgacg tcgaaacccg tatgatgagc
    57481 aactatacgc tgaagcgtga actcgccgag tttgccgcgt cgctggttca accgggcgaa
    57541 accatcttta tcgaaaatgg cagcagcaat gccctgctcg cccggactct gggggagcag
    57601 aagaaaaatg tcactatcat cacggtcagc agctacatcg cgcatttgct gaaagacgcg
    57661 ccttgtgaag ttattttgct cggtggcgtg taccagaaaa aaagcgaaag tatggttggc
    57721 ccgttgacac gccagtgcat ccaacaggta catttcagca aagcatttat tggtattgat
    57781 ggctggcaac ctgaaactgg attcaccggt cgcgatatga tgcgtaccga tgtggtgaat
    57841 gccgtgctgg aaaaagagtg cgaagcgata gtcctgactg acagctcgaa atttggtgct
    57901 gtacattcat actccatcgg tcccgttgag cgattcaatc gcgtgattac cgattcgaaa
    57961 atacgcgcca gcgatctgat gcatcttgag cacagtaaac tcaccgttca cgtcgttgac
    58021 atttaaccat ttcccttctc tacggatgat ttgcagtttg gcaaatcatc cgctctaaga
    58081 tgattcctgg ttgataatta agactattta cctgttatta acactctcaa gatataaaat
    58141 tattatcagc gatataacag gaagtcatta tcacctgcgt gatataaccc tgcgcgcgag
    58201 cggatttcac ggaataattt caccaggctt attcttagct attatagtta tagagagctt
    58261 acttccgtga atcataaatt caggagagag tattatgttt gtaacgagca aaaaaatgac
    58321 cgcggctgtt ctggcaatta ccctggcaat gtctctgagt gcctgttcta actggtctaa
    58381 acgggaccgc aacaccgcaa tcggcgcggg tgcaggggca ttaggcggtg cagtactgac
    58441 cgatggcagt acgttgggta cattaggtgg cgcagctgtc ggtggtgtta ttggtcacca
    58501 ggtcggtaaa taatcgtaaa taaatgtcga ttatcgactc gcaattataa tacgcttgat
    58561 tctgttcagt acgaaataac tgaataatac acacagtaaa atattcgaaa gccacggata
    58621 tatccgtggc tttttatgtt aaccgcctgc gagttttacc ttcatccctt tcgcttcaag
    58681 tagtgatttt aataaatcac gcttatcgcc ctggatttca ataactccat ctttaactgc
    58741 tccgccgcag ccgcattttt tcttcagttc cgctgcaagt tttgtcagtt cggcatcatc
    58801 gatatcgata ccggtaatca ggcaaacgcc cttaccttta catccactgg tctgacgctg
    58861 aatacgcacc acaccgtcgc ctttagggcg tactgggggc gcttttggct catcaatacg
    58921 tccggtttct gttgagtaga ccagacggct gttggaatca ctcatcatgc actccgctgt
    58981 aaagaggcgt tgatcgcttt cagcgtctgc gctggatcta ccgattgcgt taccgggcga
    59041 ccaatcacca tataatcaac accagccgcc aacgcctgtt ctggcgtcat aatgcggcgc
    59101 tggtcaccag cttcactccc ctgcggacga atgcccggtg taaccagttt gaactcctga
    59161 ccgaatacct gtttaaagcg cacagcttcc tgagcagaac acaccacacc atcaaggcca
    59221 catttttgcg tcagtgccgc cagacgttct gcataatctg caggtgacag tgtcacgcca
    59281 agatcggcca ggtcgctggc ttccatgctg gtcaacactg tcacagcaat caaaagcggt
    59341 gcatctttgc catacggaac cagtgcctca cgcgctgcgg tcatcatacg cgccccacca
    59401 gaggcatgaa cattcaccat ccacacgcct aagtcagctg cagcagcgac agcgtgcgct
    59461 gcagtgttgg ggatatcgtg gaatttcagg tcaagaaaga tatcaaaacc acgctgttga
    59521 agttcgcgca caaactgtgg cccaaacaat gtaaacatct ctttgccgac cttcagacga
    59581 caatcgcgtg ggtcgatctt gtcgacaaag gccagcgcgg catcacgatt atgataatca
    59641 agggcaacaa ccacaggaga attcgtaaca gcgcgggaag aagatgaagc agttaacgtc
    59701 atgaccagac cttcttgatg atgggcaccg gaagtggcgc gaaacaggta aacggcgagc
    59761 attctacccg tggaaataaa aaattaacag ttgcgatttc tcccctggca ggctttttct
    59821 gcgccgtcgt tgccggagca caatggaaca tcaataacaa gtatgttgta actaaagtcg
    59881 gattttttta aaaattacag gccatcaaga ccgcgaatcg gtttaattgt tgaccaggcc
    59941 cgacaagacg gacaatgcca gtagagggtg tatgcggtaa aaccacattt ctggcagcga
    60001 taacgaggct tactacgtac cttttcgcca accatgtcac gcagcaccat caggctctct
    60061 ttggcacgcc cttcttccgc ttcatttaag tggtaatcca ttaacttatg gaacacacgc
    60121 atggtcggat gacgctgaag ctggcgcgta atatagacct gtgcagcctc actaccgtcg
    60181 cgcgcttcga tgatatccgc aagcatcaat tcagcatcgg caccggtgtt ctcttccacc
    60241 gcgcgctgca ggaattctgc ccattcggca gttttaccca actgctgata gcaggtttgc
    60301 aacatttcca gcgtttcgct aaccagttct ctgtcctggg atatgacacg ttgcagactt
    60361 tcgacggctt tggcgtattc tcctttcgcc ataaacacgc gtcccatcat gatggatacg
    60421 cgggcgctgt ttttatctgc cgccgcccct ttttttagca aggtcatggc acgatcgaga
    60481 tcgtcgctgg ccatatgctg cagggctaac tcacagtaga aatgggcaat ttcgacgcgc
    60541 tgtttatctt tacccagctt caccaggcgt tcggcaacat caattgcttt ctgccactca
    60601 ctggtagcct ggtagatttg tagcaactgt tgcagcgcgc caatgcggaa gtcagtttca
    60661 tcggtcagct gattgaacat gtcttccgcg cggtcatata acccggcggc catgtaatca
    60721 cgccccagtt gttgaatcgc caacagacgc tgttcatagg tcagcgaggc gctttccatt
    60781 agggtctgat ggatgcgaat agcgcgatca acttcgccac gcgaacggaa caggtttccg
    60841 agcgtaaggt gggcttcaac ggtgcctgta tcctctttaa gcatatcgag aaacaggtct
    60901 accgctttat cctgttgatt actaagcagg aagttaaccc ccgctacgta atcacgcgac
    60961 aagcggttgg cttcatcttg cttgttttgt tgcgcacttc tgcggcccat ataccagcca
    61021 taggcagcgg ctacaggcaa aagcagaaac aacaactcca gcatagaaag ttattccttc
    61081 gccgctgacg agtgcggcac tacagccacg tcagtcgcgg gtgaaagctg gttttccagt
    61141 cgctttattt tacgttcagc gcgcgccagg gaaacacgaa ctcgcagcca gaacaggcca
    61201 caaatcaacc aaccgatagc aaaccccgca gcaaacaata ccgccagcaa tgtggaaata
    61261 cggtactccc cttgcgctaa cagataatta aacgtcacct gttgatcgtt ctgcgcaccc
    61321 aacgtgaccg aaatcacgaa gatcgctaac accagtaaga aaatgagtaa atatttcaca
    61381 ttacttcccg ttatgtggtt agagcgaata aattatggtc aacttaccgc aatagccctt
    61441 taaagtacca ttttcgcgcc gtctgcgaaa tggcatccac gcgcattgtt gatgatttaa
    61501 gggctaaatt cggaaaatca accagcgtta actttcttgt tctcgttgcg ctatttcgcg
    61561 attttcttcc gcaggtggtg ttaatggccc acaaattcgt tgcgcaagcc acgttgccac
    61621 cgccaccagc gcccacgaaa tcaacgtagc tactaccaga tcgcgtggcc aatgcatccc
    61681 gagcagcagg cggcttccca tgactccagt tgcccagacc aacaagatag caatggttaa
    61741 cgttcgccga cgcggccaca gcaaaccaac ggccagtagt gcccaactgg cagcaaacat
    61801 cgtgtgaccg gaaggaaagg caaaccccgt ctctttctgc cagtgtgaac gcaaatattg
    61861 tgggatattt ttctcttcag ccaactgttc tttcactaga tttccgcgtt ctgctcgctt
    61921 taaagtgtag aactcatcaa ccggaatatg atgtgttttt tccaaccaga taacaaaagg
    61981 tcgtggttcc tggactttgt ctttgatcca ggatttaacg ccttgtccaa caaggattgc
    62041 ggccgccaga atggcaaata atacaaaggc agccttaatg cgaaaacgca gacaccagag
    62101 aaaccagccg aataaaatca aatgtgtaat gacgccccag ggctgggtga cagtttcagt
    62161 aacccaaaaa gccgctttta gtagccaact ttgttctcca ggttgccaac gccagccaga
    62221 aatccatacg gctactggca taacaagcaa tagtgcagct cccactgcgg tacgtctggc
    62281 aatcgaacgc atggcctctc ctttttgata agtcccacaa tcataactga aaatgccagt
    62341 tccaggaaaa attgacagat ttgtgccatt ccgtgaacga tcgacgcgtc gtgattaggt
    62401 gaaccccttc tcgttatggc aaaataagcc aatacagaac cagcattatc tggagaattt
    62461 catgcagctt aaacgtgtgg cagaagccaa actgccaacc ccatggggcg atttcctgat
    62521 ggtgggattt gaagaactgg caaccggaca cgatcatgtc gcgctagtct atggcgatat
    62581 ttccgggcat accccggtac ttgcgcgcgt ccattccgaa tgtctgaccg gtgacgccct
    62641 gttcagcttg cgctgcgatt gtggcttcca gctcgaagcg gcattgacgc aaattgccga
    62701 ggaaggccgt ggtattttgc tgtatcaccg tcaggaaggt cgtaacattg gtctgctgaa
    62761 taaaatccgc gcttacgcac tgcaggatca aggttacgat actgtagagg ctaaccacca
    62821 gttaggcttc gccgctgatg agcgcgactt cactctttgc gctgatatgt tcaaactcct
    62881 tggcgtcaat gaagtccgct tgttaaccaa taacccgaaa aaagtcgaaa ttctgaccga
    62941 agcggggatt aatattgttg aacgcgtacc attgattgta ggtcgtaacc ccaataacga
    63001 acattatctc gataccaaag ccgagaaaat gggccatttg ctgaacaaat aaccctcttg
    63061 cattgtgtaa ttaatttgct tgccggaagc aaaataaccg gcaagcaaat acttgttact
    63121 tcaacatatt acgaatgaca taatgcaaaa tgccgtcgtt ctggtagtag gtcaactccg
    63181 tcgcggtgtc gatacgacaa cggcagggta cgacttcctg gctaccatcc gcgcgcgtaa
    63241 gcgtcaccgg aaccgtcgcg ccgggttgta gattttgcag atcgccaata tcaatcttct
    63301 cttccccggt tagccctaac gttttacgcg ttacgccttg cggaaattcc agcggcagga
    63361 tgcccatgcc aattaaattc gaacggtgaa ttcgttcaaa cgattcggca atcaccacac
    63421 gaattccaag cagacgcgga ccttttgccg cccagtcacg gcttgagcct gatccatact
    63481 ctttcccggc aattaccgcc agcggcgttt gctcctgctt atagcgcatc gcagcatcat
    63541 aaatagagac tacgtcgctg tcaggtaaat gccgcgtcat ccccccttca acgccaggca
    63601 ccatttcatt acggatgcga atattggcga aggtgccgcg catcatcact tcatggttac
    63661 cacgccgcga accgtaggag ttaaagtctt ttcgctcaac accccgacct tgtagatatc
    63721 gacccgccgg gctgtcgggc ttaatactgc ccgccggaga gatatggtca gtggtgactg
    63781 aatcccccag cattgcgagg atccgcgcac cgtgaatatc ttccacaggt gctggtgttg
    63841 cctgcatttc atcaaagaaa ggcgataaac gaatataggt tgaatcctcc tgccatccgt
    63901 aggtatcgga tcgtgcgacg ttaatttctt tccactctgc agtgccttca aaaacttctg
    63961 cgtactcttt gcggaacatt tctgtggaga cttgttctac cgcacgggca atttcttgtg
    64021 ccgatggcca gatatctttc agataaaccg gatcgccttt gcgatcatgg ccgataggct
    64081 cagaagccag gttgatattc atatttcccg ccagcgcata ggcaaccacc agcggcggcg
    64141 aggccagcca gttagtttta accagcggat ggatacggcc ttcaaagtta cggttgccgg
    64201 acagcaccgc accgacggtt aaatcgcctt ttttgattgc cgtttcgata ggatcgggca
    64261 gcggcccaga gttaccaata caggtggtac aaccgtatcc cacaaggtta aaccccagtt
    64321 cgtcgagata cggtgtcagt ttcgcttttg ccagatagtc agaaacaact ttcgagcctg
    64381 gtgccagcga cgctttgacc cacggttgcc gcttgaggcc cagagttacg gcttttttcg
    64441 ccagcaagcc tgcagccatc agcacacttg ggttagaggt gttggtgcac gaggttatcg
    64501 cagcaatgac cacagcgcca tcaggtaact gatactgatg tccgttcata acataatcga
    64561 ccggctggcg atctttatgc gtggcattca cttccagttc gttactggcg gcaaatgctt
    64621 ttggtacatc gggcagtgca acgcgatcct gtgggcgttt tggccctgcc aggctcgctt
    64681 caacgtcatt catatccagt tctaacgtac tggtaaaaat cggttcatcg cccgggttac
    64741 gccacatgcc ctgcgctttg gcgtattttt cgaccaactc gacctgatct tcactgcgcc
    64801 cgcttaaacg catgtaatcg agggtgacag catcgattgg gaagaagcca caggtggcac
    64861 catattctgg cgacatattg gcaatggtgg cgcgatccgc caacggtagt gaatccagac
    64921 catcaccata aaattcgacg aatttcccga ccacgccatg tttgcgcagc atttgggtaa
    64981 cagtgagaac caggtctgtg gcggtaatac cttcacgtaa ttttccggta agtttgaagc
    65041 ccactacgtc cgggataagc atggaaaccg gctggcctaa cattgcggct tctgcttcga
    65101 tcccaccaac gccccacccc agcacgccaa ggccgttgat catggtggtg tgcgagtcag
    65161 taccaacgag tgtatccgga taagcaatcc attcaccgtc ctgcaattca ctccacactg
    65221 ctttgccgag atattcgagg ttaacctgat ggcaaatgcc tgtgcctggc ggcacgacgc
    65281 taaaccgact gaacgcctgc tttccccatt tcaggaacac ataacgttcg tggttgcgct
    65341 ccatttccag gcgtacgttt tcttcaaatg cctcatcatc accaaaacga tcgacagtca
    65401 ccgagtggtc aatgaccagg tcgaccggtg agagcggatt aacctttgca gtatcgccgc
    65461 cgaggcgttt aaccgcttcg cgcattgccg ccagatcaac aacggcaggt acgccggtaa
    65521 agtcctgcat cagcaccctt gccgggcggt aggcaatttc acggtcagca tgggcatttt
    65581 tcagccatcc cgccagcgcg tggatatcct cttcggtaac cgagttacca tcctgccagc
    65641 gcagcaggtt ttcgagcaaa actttgagtg acttgggtag acgggtgata tcgcccagtg
    65701 atttggcagc aagcggcagg ctgtagtagt ggtaagtttt atctttggcc tgcaacgtgt
    65761 ccttactggc ttctcgtagg gttgacgaca tagctcctcc ttaatgacag ggttgcgaag
    65821 ccctgattct cttcagggtg aatattaaag ataacacaaa catcgcgtaa cgatttgata
    65881 acaacccaaa ttgataaaag aggagaaacc ttgtggcaaa gcaaatgaca accccgccgc
    65941 agcggggtca aggatcagtg agtaatgatc cagaggagtt cggcccagaa gagcatcgac
    66001 agagaaaatg cacccatcca ggaccaatat ttcatatttg tgttgatcat aggctacacc
    66061 cttaacggtt attactttgt gttgaggtaa ctcaacctgt tattgctgcg gtgtattaaa
    66121 agccctttaa ttcattaaaa atgggtaata agaatgatga taattattgt ttcggctgat
    66181 catcttcagc aaacagttct ataaaggctt gctgctgcga tgttagttgc cagtcgtctg
    66241 gaataacgac ggaacattcc cgcttcgttt ctggttcatt gtcactatcc tggcataatg
    66301 gtgacgactc cagttgctcc tgaccaacca tagttatccc cacactttcc aaatcagcag
    66361 tgcgacaacc acccagaaaa gtgcagaacc aagaaaaacc gccagccagg ctttacgttt
    66421 taattcagga tcgcggcggg gttcctgatt tccagaaggc attgctaacc tcatataatc
    66481 gccatcactt atcagcaaga cgttgaacga ttggtgtaat taatcatacg atgagacaaa
    66541 tcctaaagaa actttagtca aaaatccagc gaatttacga atcgtctcca gtgaataatt
    66601 caatcttttg acctgaaata gatatttgaa aactggaatt tgcgaggcgg gtaattagac
    66661 actactttac aggtaattta ccctataaat atgag
//
LOCUS       DAZFKF010000019        65312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFKF010000019 DAZFKF010000000
VERSION     DAZFKF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.019"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(186..665)
                     /gene="folK"
                     /locus_tag="ACHKA1_001476"
     CDS             complement(186..665)
                     /gene="folK"
                     /locus_tag="ACHKA1_001476"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO5679152.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIESQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVPQPAIW"
     gene            complement(662..2059)
                     /gene="pcnB"
                     /locus_tag="ACHKA1_001477"
     CDS             complement(662..2059)
                     /gene="pcnB"
                     /locus_tag="ACHKA1_001477"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO5679153.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(2119..3015)
                     /gene="gluQRS"
                     /locus_tag="ACHKA1_001478"
     CDS             complement(2119..3015)
                     /gene="gluQRS"
                     /locus_tag="ACHKA1_001478"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO5679154.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPGNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQVEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(3082..3537)
                     /gene="dksA"
                     /locus_tag="ACHKA1_001479"
     CDS             complement(3082..3537)
                     /gene="dksA"
                     /locus_tag="ACHKA1_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO5679155.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3715..4419)
                     /gene="sfsA"
                     /locus_tag="ACHKA1_001480"
     CDS             complement(3715..4419)
                     /gene="sfsA"
                     /locus_tag="ACHKA1_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO5679156.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4434..4964)
                     /gene="thpR"
                     /locus_tag="ACHKA1_001481"
     CDS             complement(4434..4964)
                     /gene="thpR"
                     /locus_tag="ACHKA1_001481"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO5679157.1"
                     /translation="MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCIQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            5038..7467
                     /gene="hrpB"
                     /locus_tag="ACHKA1_001482"
     CDS             5038..7467
                     /gene="hrpB"
                     /locus_tag="ACHKA1_001482"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO5679158.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRMEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGISLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVRRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7663..10197
                     /gene="mrcB"
                     /locus_tag="ACHKA1_001483"
     CDS             7663..10197
                     /gene="mrcB"
                     /locus_tag="ACHKA1_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO5679159.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10417..12606
                     /gene="fhuA"
                     /locus_tag="ACHKA1_001484"
     CDS             10417..12606
                     /gene="fhuA"
                     /locus_tag="ACHKA1_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO5679160.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTIEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIANDGHTLNRGTVVDNERLQNFSVDTQLESKFATGEVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGNAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12657..13454
                     /gene="fhuC"
                     /locus_tag="ACHKA1_001485"
     CDS             12657..13454
                     /gene="fhuC"
                     /locus_tag="ACHKA1_001485"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO5679161.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLEAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVESLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13454..14344
                     /gene="fhuD"
                     /locus_tag="ACHKA1_001486"
     CDS             13454..14344
                     /gene="fhuD"
                     /locus_tag="ACHKA1_001486"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO5679162.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14341..16323
                     /gene="fhuB"
                     /locus_tag="ACHKA1_001487"
     CDS             14341..16323
                     /gene="fhuB"
                     /locus_tag="ACHKA1_001487"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO5679163.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFLQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGVALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16358..17638)
                     /gene="hemL"
                     /locus_tag="ACHKA1_001488"
     CDS             complement(16358..17638)
                     /gene="hemL"
                     /locus_tag="ACHKA1_001488"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO5679164.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAGSVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17804..17851
                     /gene="yadX"
                     /locus_tag="ACHKA1_001489"
     CDS             17804..17851
                     /gene="yadX"
                     /locus_tag="ACHKA1_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO5679165.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17863..19284
                     /gene="clcA"
                     /locus_tag="ACHKA1_001490"
     CDS             17863..19284
                     /gene="clcA"
                     /locus_tag="ACHKA1_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO5679166.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19308..19373
                     /gene="yadW"
                     /locus_tag="ACHKA1_001491"
     CDS             19308..19373
                     /gene="yadW"
                     /locus_tag="ACHKA1_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO5679167.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19366..19710
                     /gene="erpA"
                     /locus_tag="ACHKA1_001492"
     CDS             19366..19710
                     /gene="erpA"
                     /locus_tag="ACHKA1_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO5679168.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19757..20380)
                     /gene="yadS"
                     /locus_tag="ACHKA1_001493"
     CDS             complement(19757..20380)
                     /gene="yadS"
                     /locus_tag="ACHKA1_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO5679169.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20418..21218)
                     /gene="btuF"
                     /locus_tag="ACHKA1_001494"
     CDS             complement(20418..21218)
                     /gene="btuF"
                     /locus_tag="ACHKA1_001494"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO5679170.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIEQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21211..21909)
                     /gene="mtnN"
                     /locus_tag="ACHKA1_001495"
     CDS             complement(21211..21909)
                     /gene="mtnN"
                     /locus_tag="ACHKA1_001495"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO5679171.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21993..23510
                     /gene="dgt"
                     /locus_tag="ACHKA1_001496"
     CDS             21993..23510
                     /gene="dgt"
                     /locus_tag="ACHKA1_001496"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO5679172.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23640..25064
                     /gene="degP"
                     /locus_tag="ACHKA1_001497"
     CDS             23640..25064
                     /gene="degP"
                     /locus_tag="ACHKA1_001497"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO5679173.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25219..26376
                     /gene="cdaR"
                     /locus_tag="ACHKA1_001498"
     CDS             25219..26376
                     /gene="cdaR"
                     /locus_tag="ACHKA1_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO5679174.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26465..26851)
                     /gene="yaeH"
                     /locus_tag="ACHKA1_001499"
     CDS             complement(26465..26851)
                     /gene="yaeH"
                     /locus_tag="ACHKA1_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO5679175.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27165..27989)
                     /gene="dapD"
                     /locus_tag="ACHKA1_001500"
     CDS             complement(27165..27989)
                     /gene="dapD"
                     /locus_tag="ACHKA1_001500"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO5679176.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVYIGQSTKIYDRE
                     TGEVHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28020..30692)
                     /gene="glnD"
                     /locus_tag="ACHKA1_001501"
     CDS             complement(28020..30692)
                     /gene="glnD"
                     /locus_tag="ACHKA1_001501"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO5679177.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30754..31548)
                     /gene="map"
                     /locus_tag="ACHKA1_001502"
     CDS             complement(30754..31548)
                     /gene="map"
                     /locus_tag="ACHKA1_001502"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO5679178.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31916..32641
                     /gene="rpsB"
                     /locus_tag="ACHKA1_001503"
     CDS             31916..32641
                     /gene="rpsB"
                     /locus_tag="ACHKA1_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO5679179.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32776..33627
                     /gene="tsf"
                     /locus_tag="ACHKA1_001504"
     CDS             32776..33627
                     /gene="tsf"
                     /locus_tag="ACHKA1_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO5679180.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDSGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33774..34499
                     /gene="pyrH"
                     /locus_tag="ACHKA1_001505"
     CDS             33774..34499
                     /gene="pyrH"
                     /locus_tag="ACHKA1_001505"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO5679181.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34649..35206
                     /gene="frr"
                     /locus_tag="ACHKA1_001506"
     CDS             34649..35206
                     /gene="frr"
                     /locus_tag="ACHKA1_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO5679182.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35298..36494
                     /gene="ispC"
                     /locus_tag="ACHKA1_001507"
     CDS             35298..36494
                     /gene="ispC"
                     /locus_tag="ACHKA1_001507"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO5679183.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36683..37441
                     /gene="ispU"
                     /locus_tag="ACHKA1_001508"
                     /gene_synonym="uppS"
     CDS             36683..37441
                     /gene="ispU"
                     /locus_tag="ACHKA1_001508"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO5679184.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37454..38311
                     /gene="cdsA"
                     /locus_tag="ACHKA1_001509"
     CDS             37454..38311
                     /gene="cdsA"
                     /locus_tag="ACHKA1_001509"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO5679185.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38323..39675
                     /gene="rseP"
                     /locus_tag="ACHKA1_001510"
     CDS             38323..39675
                     /gene="rseP"
                     /locus_tag="ACHKA1_001510"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO5679186.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39705..42137
                     /gene="bamA"
                     /locus_tag="ACHKA1_001511"
     CDS             39705..42137
                     /gene="bamA"
                     /locus_tag="ACHKA1_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO5679187.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVQVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42259..42744
                     /gene="skp"
                     /locus_tag="ACHKA1_001512"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42259..42744
                     /gene="skp"
                     /locus_tag="ACHKA1_001512"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO5679188.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42748..43773
                     /gene="lpxD"
                     /locus_tag="ACHKA1_001513"
     CDS             42748..43773
                     /gene="lpxD"
                     /locus_tag="ACHKA1_001513"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO5679189.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43878..44333
                     /gene="fabZ"
                     /locus_tag="ACHKA1_001514"
     CDS             43878..44333
                     /gene="fabZ"
                     /locus_tag="ACHKA1_001514"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO5679190.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44337..45125
                     /gene="lpxA"
                     /locus_tag="ACHKA1_001515"
     CDS             44337..45125
                     /gene="lpxA"
                     /locus_tag="ACHKA1_001515"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO5679191.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45125..46273
                     /gene="lpxB"
                     /locus_tag="ACHKA1_001516"
     CDS             45125..46273
                     /gene="lpxB"
                     /locus_tag="ACHKA1_001516"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO5679192.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTEYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46270..46866
                     /gene="rnhB"
                     /locus_tag="ACHKA1_001517"
     CDS             46270..46866
                     /gene="rnhB"
                     /locus_tag="ACHKA1_001517"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO5679193.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            46903..50385
                     /gene="dnaE"
                     /locus_tag="ACHKA1_001518"
     CDS             46903..50385
                     /gene="dnaE"
                     /locus_tag="ACHKA1_001518"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO5679194.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50398..51357
                     /gene="accA"
                     /locus_tag="ACHKA1_001519"
     CDS             50398..51357
                     /gene="accA"
                     /locus_tag="ACHKA1_001519"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO5679195.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51456..53597
                     /gene="ldcC"
                     /locus_tag="ACHKA1_001520"
     CDS             51456..53597
                     /gene="ldcC"
                     /locus_tag="ACHKA1_001520"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO5679196.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53654..54043
                     /gene="yaeR"
                     /locus_tag="ACHKA1_001521"
     CDS             53654..54043
                     /gene="yaeR"
                     /locus_tag="ACHKA1_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5679197.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54108..55406
                     /gene="tilS"
                     /locus_tag="ACHKA1_001522"
     CDS             54108..55406
                     /gene="tilS"
                     /locus_tag="ACHKA1_001522"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO5679198.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTPQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGEKGVSFVWQKTLS"
     gene            complement(55455..55715)
                     /gene="rof"
                     /locus_tag="ACHKA1_001523"
     CDS             complement(55455..55715)
                     /gene="rof"
                     /locus_tag="ACHKA1_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO5679199.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55702..55902)
                     /gene="yaeP"
                     /locus_tag="ACHKA1_001524"
     CDS             complement(55702..55902)
                     /gene="yaeP"
                     /locus_tag="ACHKA1_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO5679200.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56068..56613
                     /gene="yaeQ"
                     /locus_tag="ACHKA1_001525"
     CDS             56068..56613
                     /gene="yaeQ"
                     /locus_tag="ACHKA1_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO5679201.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56610..57032
                     /gene="arfB"
                     /locus_tag="ACHKA1_001526"
     CDS             56610..57032
                     /gene="arfB"
                     /locus_tag="ACHKA1_001526"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO5679202.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKELTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57046..57756
                     /gene="nlpE"
                     /locus_tag="ACHKA1_001527"
                     /gene_synonym="cutF"
     CDS             57046..57756
                     /gene="nlpE"
                     /locus_tag="ACHKA1_001527"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO5679203.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQVAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(57956..58780)
                     /gene="yaeF"
                     /locus_tag="ACHKA1_001528"
     CDS             complement(57956..58780)
                     /gene="yaeF"
                     /locus_tag="ACHKA1_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO5679204.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDVEAKTWAV
                     KFQHQSSFTEQAIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQICSLNPFSADFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(58833..60551)
                     /gene="proS"
                     /locus_tag="ACHKA1_001529"
     CDS             complement(58833..60551)
                     /gene="proS"
                     /locus_tag="ACHKA1_001529"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO5679205.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVNAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60662..61369)
                     /gene="tsaA"
                     /locus_tag="ACHKA1_001530"
     CDS             complement(60662..61369)
                     /gene="tsaA"
                     /locus_tag="ACHKA1_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO5679206.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MTVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61366..61770)
                     /gene="rcsF"
                     /locus_tag="ACHKA1_001531"
     CDS             complement(61366..61770)
                     /gene="rcsF"
                     /locus_tag="ACHKA1_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO5679207.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61888..62703)
                     /gene="metQ"
                     /locus_tag="ACHKA1_001532"
     CDS             complement(61888..62703)
                     /gene="metQ"
                     /locus_tag="ACHKA1_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO5679208.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62743..63396)
                     /gene="metI"
                     /locus_tag="ACHKA1_001533"
     CDS             complement(62743..63396)
                     /gene="metI"
                     /locus_tag="ACHKA1_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO5679209.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63389..64420)
                     /gene="metN"
                     /locus_tag="ACHKA1_001534"
     CDS             complement(63389..64420)
                     /gene="metN"
                     /locus_tag="ACHKA1_001534"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO5679210.1"
                     /translation="MIKLSNITKVFHQGTRIIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64608..65183
                     /gene="gmhB"
                     /locus_tag="ACHKA1_001535"
     CDS             64608..65183
                     /gene="gmhB"
                     /locus_tag="ACHKA1_001535"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO5679211.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFINGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15468 a        16169 c        17967 g        15708 t
ORIGIN      
        1 cctaatctat ttgcatgaag tgctatttca tgaatgaatt aaaaaaaaac taaaatgtaa
       61 attaagtata tttaatgtgt aaggggggat gtgaggagta agataagaaa ataatttttc
      121 ttggtggatg aaatagccgt atcattttgt attcctctcg tcaagaaaat aaaataatac
      181 agctattacc agatggcagg ttgtggtact gctagtgaag tgagtatttc acgtaatgtt
      241 tccccatcag gaaacaccaa ctccggcgcg atttcaaaca gcggccacag cataaatcca
      301 cgatttttca tatcgtagtg cggaacggtc aggcgttcgg tatttattac ttcattacca
      361 aacagcatga tgtcgagatc cagcgtgcgc ggtccccagc gttcagcttt acggacgcga
      421 ccttgctgcg attcaatacg ctgtgtgtga ttgagtagct cttcaggtgc aagagaggtt
      481 tccagcgcta cggctgcatt taagtaatcg ggttgatctt gcggccccag cggtggagtg
      541 cggtaaaacg aagaaacggc aagaatgtgg ctttcaggga tatcgcctaa tgctttcagg
      601 gcagcattga cctgctccag cggagaggcc agattgctgc ctatggcaat atacgccact
      661 gtcatgcggt accctcacga cgtggtgcgc gtttgcgtgg acgacgagta cgacgacgcg
      721 gtgacggttc ttcatccagc tcgttgagca tccctttttg gtctggtggc gcggaaacct
      781 ggaactcacc ccaccatttc accagacgct gcagttcagc gttacgttca acttcagctc
      841 gcaaggccaa caggtcataa gccgcacgga acttaggatg ctccagcagt ttccatgcac
      901 gtttaccctg acgtcgggac atacgcaact gcaactgcca gatatcgcgg gttaacgtcg
      961 tcagacgttt cgggattgcc agtgaacggc aggcttcgtc cagcacgtcg ttcatcgcca
     1021 gcgcgaaagc gtcgtgatag gtcaggccgc tttcctgggc gatcttctgt gccgtctcca
     1081 gcagtgggta ccagaacatg gcggcaaaca aaaacgccgg gttaacgcgc atatcgttat
     1141 gaatacgcgt atcggtgttc ttcagtacct gctcaatgat ccgctccatt gggctgtcgc
     1201 cattttccgt gaagtagcgg gtaatggtcg ggaacagcgg ctggaacaga tgatattcac
     1261 acagcagctt ataggtttcg taaccgtagc ccgcttgtag cagtttaagc gattcttcaa
     1321 acaggcgtgc cggtgggata tcgttcagca gggtagcgag gcgagggatc ggttctgcgg
     1381 tttccgggct gatgcgcata cccaatttgg cggcaaaacg tactgcgcgc agcatacgta
     1441 ccggatcttc acggtagcgc gtttccgggt taccaatcag acggataaca ccgtccttca
     1501 gatccttcat accgccaacg taatcacgga cggtaaaatc cgctacgctg taatacaggc
     1561 tgttgatagt gaaatcgcgg cgctgggcgt cttcttcgat ggagccgaaa atgttgtcgc
     1621 gcagcaacat gccgttttgc ccgcgttggg aggtcgtgcg gtcgctgacg ttaccttcgt
     1681 ggtgtccacg gaaggtcgca acttcgataa tctccgggcc aaacatcaca tgagccagac
     1741 ggaaacggcg acccaccagg cggcagttac ggaacagttt gcgcacctgc tcaggcgtgg
     1801 cgttagtggt tacgtcaaag tctttcggct ttttgccaag taacaggtcg cgcacgccgc
     1861 cgccaaccag ccaggcttcg tatcccgctt tattgagcct gtacattacc ttcagggcat
     1921 tttcactgat atctttgcgg gaaatagcat gctgctcacg cgggatcacc gtcacctgtg
     1981 gacgggcgac tgcctgttca gcctcgcttt cctcgcggct tagcaccttg cggcaaaaat
     2041 tagcgactcg ggtaaaaata gtacacctcg gtagtgtcaa acatcattca ggacaaaaaa
     2101 atagcggcta atcatagctc agcatgacgc atttgagaat gttgaattta caattgccga
     2161 ctcaggcact gcggtaagcc gccagttttt gacggctgac tgaaggattt gttcgacgct
     2221 gaaatcctgc cagtgtgctt ctacctgctg acccagaaat tgaagtgccg cgattagtac
     2281 cgggcgtgga tcgcctttcg gcaacgcagg cgcatgattc tgcttggaaa gtttagcgcc
     2341 ttgtggatta agcgccagcg gcagatgaat gtaatctggc actttccagc caaaaagctg
     2401 gtacagcgag atttgccgca ctgtcggttc gatcaaatcc gccccgcgga caatttcgct
     2461 cacgccctgg aagtgatcat ccaccaccac cgccaggtta taggcaaaca aaccatcacg
     2521 gcgatgaata ataaaatctt cccgtgccag tttttcgtcg gcgtgaataa tgcctcgcag
     2581 caggtcagta aactgggtga ccggatgctg ctggcggata cgcactgcgg cgttgcctgg
     2641 tccatgatgc aattcccggc aatgaccgtc gtaaataccg ccaatgcttt gaatacgtgc
     2701 acgcgtgcag gtgcagtaat aacttagtcc ttgctcatgt aaccaggcga gtgcttcgcg
     2761 ataggcgtgg tgacgttgcg attgccagag aacatcgccg tcccagtgca ggccgtaatg
     2821 ttccagctgg cgcaggatag tttctgcggc accgggaact tcacgaggcg ggtcgatatc
     2881 ttctatgcgt accagccagc gaccttgccg ggcgcgagcc tgcaaatagc tgccaagcgc
     2941 agcaatcaga gaaccaaaat gaagctcgcc ggaaggagag ggggcgaagc ggccaatata
     3001 gtgtgtgtct gtcatctctt tgaacaaaaa ataaggcggg agcatttccc gcctgtggta
     3061 aacgtgatgg aacggctgta attagccagc catctgtttt tcgcgaattt cagccagcgt
     3121 tttgcagtcg atgcacagat cggctgtcgg gcgcgcttcc agacggcgaa taccaatttc
     3181 aacaccgcag gattcacagt agccgaaatc ttcgtcttcc acttttttca gcgtcttctc
     3241 gatctttttg atcagcttac gctcgcgatc gcggttacgc agttcgaggc tgaactcttc
     3301 ttcctgggct gcacggtcta ccgggtccgg gaagttggct gcttcatcct gcatatgtgt
     3361 aacggtgcga tcgacttcat ccctgagttg attacgccat gcttccagaa tacgacggaa
     3421 gtgcgccagc tgggcttcat tcatatactc ttcgcccggc ttctcctgat atggttccac
     3481 cccagcgatg gcgagaatac tcagggacga tgttttacgg ttttgccctt cttgcatgtt
     3541 gcttctcctt aacacgcact atcgatcccc atgttcgggg gaaaaatgag gccgctataa
     3601 atagcagatg cttttccgga tagcaattat ctaaacgtaa cacttgacaa ctgtgtgagg
     3661 aaaagcgtat ttgcgcacgc gaccagaatg taaattaacc agttacttac tttactacaa
     3721 tgtaaccggc agtgattttt taagagccat gccttcagca gaaatttccg ctttgtaagc
     3781 cagaatttct acccccctct gttgagcttc tgacaatagt tgcgcgtatt tctcatcgat
     3841 gtggcgcgcg ggtgaaaacc gtgtaatggc tgaatgcagc acggcgaaaa agataaccgc
     3901 acgctggcct tcagccgcta cgctcatcaa ctcccgaagg tgtttctgac ctcgttcagt
     3961 gaccgcatcg ggaaaatatc cctgttcgtt ctccgctaac gtaaccgatt tcacttcaat
     4021 atagcagtct ggacgcgaat ccgcctgcaa cataaagtca atacggctgc gttctgcgcc
     4081 gtattttact tcgcttttca gcgagctata gcctgacagt tctgaaattg attcattaag
     4141 gatagcctct ttcgtcaacc tgttagccca aagcgtgttg acgcaaataa atgcgccgct
     4201 ctggctttga gttaattccc aggtgtgtgg gtatttccgt ttagtgttgt ctgaagtcga
     4261 ataccagacg gtatcgccag gcgttgcaca accggtcatc gcacccgtat tcgggcagtg
     4321 tagcgttaat tcgcgaccat cgggtgtgat cacatcggct aaaaaacgtt tgtaacgctg
     4381 aattagcgtc gcgcgctgta gagggggaga aaattccatt gcgacaatcc ttgttattgc
     4441 gttagcgccc agcgttttag cggcgtgtag cgtgtgcgtc cacgggcaaa cgaggaggcg
     4501 taaagggtga actccgtcac cgcatacgac cagttaaaac ctggcggcgg gattgtcacc
     4561 gcctcgctgg cgtcgcgcaa taaggtaata tgtggatgaa acggacgatt gctttgaata
     4621 caaccgctgc gggcagcctg tgaacggagc atattcgcca gctggattaa gccgcgtgga
     4681 ggctgacgca tccctaacca caccacacgc gaacgcagcc attgtccggc gtcatcaagc
     4741 gtgagtgtga aaccaggttg acgaatccgt ccggctaaaa gagaaagcgc cttctctttc
     4801 tcggcgctca cttcgcctaa aaatgccaga gtcagatgca aattatcggc ggcgaccgga
     4861 cgtcccgcct caagtgggaa gtgtgtggcg cgccagtgga taatctgttc gcggatttct
     4921 gcaggtaagt cgatagcaaa gaacagacgt tgcgggtcag acatgtgagg cactcggtta
     4981 tgaattaccg cgatgctaca atgtggcgcg aagaatgtta accctctgga gcgttttgtg
     5041 tcgtcgttgc ccgttgctgc cgtcttacct gaattactta ccgctctcga ttgtgcgccg
     5101 caggtattat taagtgcgcc gaccggggcc gggaaatcaa cctggctgcc gctgcaactg
     5161 ctggcgcatc ccggcattaa cgggaaaatt atcctgctgg agccgcgtcg tctggcggcg
     5221 cgtaacgtcg cgcaacggct ggcggaactg cttaacgaaa agccaggcga taccgttggc
     5281 taccggatgc gtgcgcaaaa ctgcgtcggg ccgaataccc gcatggaagt agttaccgaa
     5341 ggcgtgctga cgcggatgat ccagcgtgac ccggaactga gcggtgttgg actggtgatc
     5401 cttgatgaat ttcatgagcg cagcttgcag gcggatttgg cgttggcgct gttactcgat
     5461 gtgcaacaag gtctgcgtga tgaccttaaa ctgctgatta tgtcggctac gctggataac
     5521 gaccgcttgc agcaaatgct gccagaagcg cccgtcgtca tctcagaagg gcgctcgttt
     5581 cctgttgaac gccgttattt accgctgccc acgcatcagc gttttgacga agccgtcgcg
     5641 gttgccaccg ccgaaatgct gcgtcaggaa agtggatcat tactgttatt tttacctggc
     5701 gtcggagaaa ttcagcgtgt gcaggaacaa ctggcttcgc gcatcggcag tgatgtgttg
     5761 ctctgcccgc tgtatggcgc gttgtcgctg aacgatcagc gaaaagcgat cctcccggca
     5821 ccgcaaggga tgcgcaaagt ggtgctggcg accaatattg ctgaaaccag tttaaccatt
     5881 gaaggtatta gtctggtggt ggattgtgcc caggagcgtg tggcgcgttt tgatccgcgc
     5941 acggggctta cgcgactgat tactcaacgc gttagccagg catccatgac gcagcgtgcc
     6001 gggcgcgccg ggcgtctgga gccgggtatc tgcctgcatt taatcgccaa ggaacaagca
     6061 gaacgcgccg cggcgcaaag tgaaccggag atcttacaaa gcgatctttc cggtttgctg
     6121 atggaattac tgcaatgggg atgcagcgat ccggcgcaga tgagctggct ggatcaaccg
     6181 ccaacggtga atctactggc cgcgaaacgt ctgttacaaa tgctgggggc gctggacggt
     6241 gaacggctta gtgcgcaagg gcaaaaaatg gcagcgctgg gtaacgatcc gcgtttagcg
     6301 gcaatgctgg tgagcgcgaa gagcgacgac gaagctgcta ccgcggcaaa aattgccgcc
     6361 attctcgaag agccgccacg gatgggcaat agtgacctgg gcgtggcgtt ttcgcgcaat
     6421 caaccagcct ggcagcaacg tagtcagcaa ctgttaaaac gcttaaacgt acgcggcggt
     6481 gaggcagaca gttcgcttat cgcgccgcta cttgccgggg cgtttgccga tcgcattgct
     6541 cgccgccgtg ggcaagatgg acgctatcaa ctggcaaacg gcatgggagc gatgctcgat
     6601 gccgacgacg cgctaagccg ccacgaatgg ttgatcgcac cgttattatt gcagggcagc
     6661 gcctcgccgg atgcgcggat tttactggcg ctaccggtcg atattgatga attagtgcga
     6721 cgctgtccgc agctggtgca gcaatctgac accgtggaat gggatgacgc gcaaggtacg
     6781 ctgaaagcct ggcgtcggct gcaaatcggt cagttgacgg tgaaagtgca gccgttggcg
     6841 aaaccgtcag aagacgagtt gcatcaggcg atgcttaacg gcatccgtga taaaggttta
     6901 agcgtgctca actggacggc ggaagcggaa cagctacgct tgcgtttgtt atgcgccgga
     6961 aagtggttgc cagaatatga ctggccagcg gttgatgatg aaagtttgtt ggcgacgctg
     7021 gaaacgtggc tgctgccaca tatggccggt gtacattcac tacgcggcct gaaatcactc
     7081 gacatttatc aggcactacg cggattactt gattggggaa tgcagcaacg tctggatagt
     7141 gaattgcctg cgcattacac tgtgccgacg ggaagccgga tcgccattcg ttatcatgaa
     7201 gataacccgc ccgcgctggc ggtgagaatg caggagatgt ttggtgaggc caccaatccg
     7261 acgatcgccc aggggcgcgt gccgctggtg ctggagttgc tttcccctgc ccaaaggccg
     7321 ctgcaaatca cgcgtgattt gggggccttc tggaaagggg cgtatcgtga ggtgcaaaag
     7381 gagatgaaag ggcgttatcc caaacacgtc tggccggacg acccggcaaa cacggcaccg
     7441 acgcgacgga cgaaaaagta ttcgtaaggt tggttttctc cctctccctg tgggagaggg
     7501 ccggggtgag ggcatcagcg cgcacgttca ccctcaccct aaccctctcc cttaagggag
     7561 aggggaccga tcgagcacaa attttgagag atatcttctt ctgtcttgta acagaagaac
     7621 agaaaatcgg gcttttgcgc ctgaatattg cggagaaaaa gcatggccgg gaatgaccgc
     7681 gagccaattg gacgcaaagg gaaaccgacg cgtccggtca aacaaaaggt aagccgtcgt
     7741 cgttacgaag atgacgatga ttacgacgat tatgatgact atgaggatga agaaccgatg
     7801 ccgcgcaaag gtaagggcaa aggcaaaggg cgtaagcctc gtggcaaacg cggctggcta
     7861 tggctgctgt taaaactggc tatcgttttt gccgtgctga tcgccattta cggcgtttat
     7921 ctcgatcaaa aaattcgtag ccgtattgat ggcaaggtct ggcaactgcc tgcggcagtt
     7981 tatggtcgaa tggtcaacct tgagccagac atgaccatca gcaagaacga aatggtgaag
     8041 ctgctggagg caacccagta tcgtcaggtg tcaaaaatga cccgtcctgg cgaatttacc
     8101 gtgcaggcca acagcattga gatgattcgc cgtccgtttg atttcccgga cagtaaagaa
     8161 ggacaggtgc gcgcgcgtct gacctttgat ggcgatcatt tggcgacgat cgtcaatatg
     8221 gagaacaacc gtcagttcgg tttcttccgt cttgatccgc gtctgatcac catgatctcc
     8281 tcaccaaacg gtgagcagcg tttgtttgtg ccgcgcagtg gtttcccgga tttgctggtg
     8341 gatactttgc tggcgacaga agaccgccat ttctacgagc atgatggaat cagtctctac
     8401 tccatcggac gtgcggtgct ggcaaacctg acagccggac gcacggtgca gggggcgagt
     8461 acgctgacgc aacagctggt gaaaaacctg ttcctctcca gcgagcgctc ttactggcgt
     8521 aaagcgaacg aagcttacat ggcgctgatc atggacgcgc gttacagcaa agaccgtatt
     8581 cttgagctgt atatgaacga ggtgtatctc ggtcagagtg gcgacaacga aatccgcggc
     8641 ttcccgctgg cgagcttgta ttactttggt cgcccggtag aagaattgag cctcgatcaa
     8701 caggcgctgt tagtcggtat ggtgaaagga gcgtccatct acaacccgtg gcgtaacccg
     8761 aaactggcgc tggagcgacg taatctggta ctgcgtctgc tgcaacagca acagattatt
     8821 gatcaagaac tctatgacat gttgagtgcg cgtccgctgg gtgttcagcc gcgcggtggg
     8881 gtgatctctc ctcagccagc ctttatgcaa ctggtgcgtc aggagcttca ggctaaatta
     8941 ggcgataagg taaaagatct ctccggcgtg aagatcttca ctacctttga ctcggtggcc
     9001 caggacgcgg cagaaaaagc cgccgtggaa ggcattccgg cacttaagaa acagcgtaag
     9061 ctgagcgatc tggaaaccgc gattgtggtc gtcgaccgct ttagtggtga agttcgtgcg
     9121 atggtcggag gttctgagcc gcagtttgcg ggctacaacc gtgcgatgca ggcgcggcgt
     9181 tcgattggtt cccttgcaaa accagcgact tatctgacgg ctttaagcca gccgaaaatc
     9241 tatcgtctga atacgtggat tgcggatgcg ccaatcgcgc tacgtcagcc gaatggccag
     9301 gtctggtcac cgcagaacga tgaccgtcgt tatagcgaaa gcggcagagt gatgctggtg
     9361 gatgcgttga cccgttcgat gaacgtgccg acggtaaatc tggggatggc gctggggctg
     9421 cctgcggtta cggatacctg gattaaactg ggcgtgccga aagatcagct gcatccggtt
     9481 ccggcgatgc tgctgggggc gttgaactta acgccaatcg aagtggcgca ggcattccag
     9541 accatcgcca gcggcggtaa ccgtgcaccg ctttctgcgc tgcgttcggt aattgcggaa
     9601 gatggcaaag tgctgtatca gagcttcccg caggcggaac gcgctgttcc ggcgcaggcg
     9661 gcgtatctga cactatggac catgcagcag gtggtacaac gcggtacggg tcgtcagctt
     9721 ggggcgaaat acccgaacct gcatctggca gggaaaacag ggactaccaa caataacgta
     9781 gatacatggt ttgcgggcat tgacggcagc acggtgacca tcacctgggt cggccgtgat
     9841 aacaaccagc cgaccaaact gtatggtgcc agcggggcaa tgtcgattta tcagcgttat
     9901 ctggctaacc agacgccaac gccgctgaat cttgttccgc cagaagatat tgcagatatg
     9961 ggcgtggact acgacggcaa ctttgtttgc agcggtggca tgcgtgtctt gccggtctgg
    10021 accagcgatc cgcaatcgct gtgccagcag agtgagatgc agcagcagcc gtcaggcaat
    10081 ccgtttgatc agtcttctca gccgcagcaa cagccgcaac agcaacctgc tcagcaagag
    10141 cagaaagaca gcgacggtgt agccggttgg attaaagata tgtttggtag taattaacgt
    10201 ctaaacgtga cataccggat ggcgagttgc catccggtaa aataacatcc cacctaaagt
    10261 attaaccctt tcttttcatc tggttgttta ttaacccttc aggaacgctc agattgcgta
    10321 ccgcttgcga acccgccagc gtttcgaata ttatcttatc tttataataa tcattctcgt
    10381 ttacgttatc attcacttta catcagagat ataccaatgg cgcgttccaa aactgctcag
    10441 ccaaaacact cactgcgtaa aatcgcagtt gtagtagcca cagcggttag cggcatgtct
    10501 gtttatgcac aggcagcggt tgaaccgaaa gaagacacta tcaccgttac tgctgcacct
    10561 gcgccgcaag aaagcgcatg ggggcctgct gcaactattg cggcgaagca ctctgctact
    10621 gcgactaaaa cggatacacc aattgaaaaa acgccgcagt ctatttcggt tgtgacaaat
    10681 gaagagatgc agatgcatca atttcagtct gtaaaagaag cattaggtta tacacctggt
    10741 gttacggtta gcagtcgtgg tgcttctaat acatatgatt ttgttatcat tcgtggtttc
    10801 tcatctgttg gtctgaatca aaataactac cttgatggac taaaacttca gggcaacttt
    10861 tataacgatg ctgtgattga tccttacatg ctcgagaggg ttgaactgat gcgtgggccg
    10921 acgtctgttc tctatggaaa aagtaatcct ggtgggatta tttctatggt gagtaagcgt
    10981 ccgaccattg aacccctgaa agaaattcag tttaaaatgg ggacggataa tctgtttcag
    11041 accggattcg atttcagtga tgcgctggat gataacggtg agttctccta ccgtttgacg
    11101 gggctcgctc gttcaacaaa cgaacagcag aaaaattctg aatctcagcg ttataccatt
    11161 gctccatcat tctcatggcg tccggacgac aaaaccaatt ttactttcct gtcctatttc
    11221 cagaatgaac cagaaacggg ttattacggt tggttgccga aagaggggac cgttgagcca
    11281 ttgcctaatg gtaaacgtct accgactgac ttcaatgaag gtgcatcgaa taatacatac
    11341 tcccgtaacc agaaaatggt gggatatagt tttgaacatg gttttaatga caccttcacc
    11401 gtgcgtcaga atctgcgttt cagtgaaatg aaaacctcac agaaaagtgt ttatggcaca
    11461 gggattgcca acgatggtca tactctaaac cgcgggacag tggtggataa tgagcgtctg
    11521 caaaacttta gcgttgatac ccaacttgaa agtaaatttg ctacaggtga agtcgagcat
    11581 actttgctga caggggtaga cttcatgcgt atgcgcaatg atatcaatgc cagctttgga
    11641 tctgcaccat ccatcgatct ttataacaaa tatcatcctg aatactttgc atttggtaac
    11701 gcagagccat accaaatgaa tgaaagcaaa caaacaggta tttatgttca ggatcaggcg
    11761 gaatggaata aatgggtatt cactctgggg ggacgctacg attggtctaa gcaagcgacc
    11821 actgttcgtg aaaactctta tacgccgact gaaggttata ttgagcgtaa tgatcatcag
    11881 ttcacctggc gcggtggtgt aaactactta ttcgataatg gtatttcacc ttactttagc
    11941 tatagccagt cctttgaacc gagtgctttc gatctgtgga gcaacccgcg cgtttcctat
    12001 aagccatcga aaggtgaaca gtatgaagct ggcgtaaaat atgttccgaa tgatatgccg
    12061 gtcgttgtta cgggcgcagt ctatcaattg acgaaaacaa ataacctgac agcagaccca
    12121 acaaacccgt tagcgcaagt cccagcaggt gagattcgcg ctcgtggtgt ggaacttgaa
    12181 gcaaaagcgg cgttaaatgc caatattaac ttgacggctt cttataccta cacggatgcg
    12241 gaatacacca aagacaccaa tctcaaaggt aaaactcctg aacaagtacc ggagcatatg
    12301 gcatctctct ggggggatta taccttcaat gaagggccgc tttctggttt aacattggga
    12361 acaggtggtc gttttattgg ttccagctat ggtgatccgg caaacacttt taaagtgggt
    12421 agcgcagctg taatggatgc tgttgtaaaa tatgatctgg cacgctttgg tatggcggga
    12481 tccagccttg ctgtgaacgt caacaatttg ctcgatcgtg agtatgttgc cagttgcttc
    12541 cagacctatg gctgcttctg gggcgcagaa cgtcaggtcg ttgcaaccgc aaccttccgt
    12601 ttctaatttt ctctttgggg cacgggtttc cgtgcccgct tcacaagttg gctgttatgc
    12661 aggaatacac gaatcattcc gataccactt ttgcactgcg taatatctcc tttcgtgtgc
    12721 cagggcgcac gcttttgcat ccgctgtcgt taacctttcc tgccgggaaa gtgaccggtt
    12781 tgattggtca caacggttct ggtaaatcca cgctgttaaa aatgttgggc cgtcatcagc
    12841 cgccgtcaga aggtgaaatt cttcttgaag cccagccgct ggaaagctgg agtagcaaag
    12901 cgtttgcccg caaagtggct tatttgccgc agcaacttcc tccggcagaa gggatgactg
    12961 tgcgtgaact ggtggcgatt gggcgttacc cgtggcatgg cgcgctgggg cgctttggtg
    13021 cggcagatcg cgaaaaggtc gaggaagcta tctcgctggt tggcttaaaa ccgctggcgc
    13081 atcggctggt agaaagcctc tctggcggcg aacgtcagcg ggcgtggatc gccatgctgg
    13141 tggcgcagga tagccgttgt ctgttgctcg acgaaccgac ttcggcgttg gatatcgccc
    13201 accaggttga tgtgctggcg ctggtgcacc gtttaagtca ggagcgtggc ctgacggtca
    13261 ttgccgtgct gcacgatatc aatatggctg cgcgttactg tgattatctg gtcgccctgc
    13321 gcggcggtga aatgattgct cagggaacgc ctgcggaaat tatgcgcggc gaaaccctcg
    13381 aaatgattta tggcatcccg atgggtattc tgccgcatcc ggcgggtgct gcacctgtga
    13441 gttttgttta ttgatgagcg gcttacctct tatttcgcgc cgtcgactgt taacggcgat
    13501 ggcgctttct ccgttgttat ggcagatgaa taccgcccac gcggcggcta ttgacccaaa
    13561 tcgtattgtg gcgctggagt ggctgccggt ggaattacta ctggcgctcg gcatcgtgcc
    13621 ttacggcgtg gcggacacca tcaactatcg cctgtgggtc agcgaaccac cactacctga
    13681 atcggtgatt gacgtcggtt tgcgtacaga acctaacctc gaactgctga ccgaaatgaa
    13741 accgtcgttt atggtctggt cggcgggata tggtccttcg ccagaaatgc tggctcgtat
    13801 tgcgccgggg cgtggattta acttcagcga cggcaaacag ccgctggcga tggcgcgtaa
    13861 atcgctgacg gaaatggcgg atttacttaa cctgcaaagc gcagcagaaa cgcatttagc
    13921 gcaatatgaa gactttatcc gcagcatgaa accccgcttt gtgaagcgtg gtgcgcgtcc
    13981 gttattgctg acgacgctta tcgatccgcg ccatatgctg gtcttcggtc caaacagctt
    14041 gttccaggaa attcttgatg agtacggcat cccaaatgcc tggcaagggg aaaccaactt
    14101 ctggggcagt accgccgtca gcatcgatcg tctggcagcg tataaagacg ttgatgtgct
    14161 ctgttttgat cacgacaaca gcaaagacat ggatgcgcta atggcaacgc cgctgtggca
    14221 ggccatgccg tttgtccgcg ccggacgctt tcagcgcgta cccgcagtct ggttttatgg
    14281 tgcaacgctc tcggcaatgc actttgtgcg cgttctggat aacgccattg gaggtaaagc
    14341 gtgagtaaac gtattgcgct tttcccggcg ttattgctgg cgctgctggt gattgtcgct
    14401 acggcgctga cctggatgaa ctttttgcag gcgctgccgc gtagccagtg ggcgcaggcc
    14461 gcctggtcgc cggatattaa cgtcatcgag cagatgattt ttcactacag cttgttaccg
    14521 cgtctggcga tttctctact ggtgggcgcg ggcctggggc tggtgggcgt gctgtttcag
    14581 caagtgctgc gtaacccgct ggcggaaccg acgacgttgg gcgttgctac aggcgcgcaa
    14641 ctggggatta ccgtcacgac gctctgggcg atccctggtg cgatggcgag ccagtttgct
    14701 gcgctggcag gggcttgtgt tgttggctta attgtctttg gcgtcgcgtg ggggaaacgg
    14761 ctgtcgccgg taacgctgat tctcgcgggg ttggtagtga gcctttattg cggcgcaatc
    14821 aatcagttac tggttatctt ccatcatgac caactgcaaa gcatgtttct gtggagcact
    14881 ggaacgctga cgcaaaccga ctggggcggt gttgagcgtt tatggccgca gttgctgggc
    14941 ggtgtgatgc tgacgttgct gctacttcgt ccgttaacac tgatggggct tgatgatggc
    15001 gtggcgcgca atctcgggct agccttgtcg ctggcgcgtc tggcggcgct gtcgctggct
    15061 attgtcatca gtgcgctgct ggtgaacgcg gtggggatta tcggctttat cgggttgttc
    15121 gcgccgttgc tggcaaaaat gctgggggcg cggcgtctgc tgccgcgact gatgctggcg
    15181 tcgttgattg gtgcgctgat cctctggctt tccgatcaaa tcatcctctg gctgactcgc
    15241 gtgtggatgg aagtgtccac cggttcggtc actgcgttga tcggtgcgcc gctgctactg
    15301 tggttgctgc cgcgtttacg cagcattagc gcgccggata tgaaggtcaa cgatcgtgtc
    15361 gcggctgaac gccaacatgt gctggcgttt gccctcgcgg gcggcgtgct gctgttgatg
    15421 gctgtggtgg tggcgctgtc gtttggtcgt gatgcgcacg gctggacgtg ggcgagcggg
    15481 gcgttgctcg atgatttaat gccctggcgc tggccgcgaa ttatggcggc gctgtttgcg
    15541 ggcgtcatgc tggcggtggc gggctgtatt attcagcggc tgaccggcaa cccgatggca
    15601 agcccggaag tgctggggat tagctctggc gcggcgtttg gcgtggtgtt aatgctgttt
    15661 ctggtgccgg gtaatgcctt tggctggctg ttacccgcgg gcagtatcgg tgccgcggtg
    15721 acgctgttga ttattatgat cgccgccggg cgcggtggat tttccccaca ccgcatgtta
    15781 ctggcgggga tggcgttaag taccgcattc accatgcttt taatgatgtt gcaggcgagt
    15841 ggtgacccgc gaatggcgca agtgctgacc tggatctctg gttcgaccta caacgccacc
    15901 gatgcgcagg tctggcgcac cggaattgtg atggtgattt tgctggcgat taccccgctg
    15961 tgccgccgct ggctgaccat tttaccgctg ggtggtgata ccgcgcgggc cgtgggggtg
    16021 gcgttaacgc cgacgcgaat tgcgctgctg ctgttagcgg cttgcctgac ggcgaccgcg
    16081 acgatgacca ttggaccgtt gagctttgtt ggtttaatgg caccgcatat cgcgcggatg
    16141 atgggctttc gacggacgat gccgcacatc gtgatttcgg cgctggtggg tggtttactg
    16201 ctggtgttcg ctgactggtg tgggcggatg gtgctgtttc cgttccagat cccggcgggg
    16261 ctgctgtcga cctttatcgg cgcgccatat tttatctatt tgttgagaaa gcagagccgt
    16321 taataaaatg cctcgcccgg tgggcgaggc aaaatcatca caacttcgca aacacccgac
    16381 gtgcagcatc gatggtgtta ttgatatctt ccatgctgtg cgccacagac ataaagcccg
    16441 cttcaaacgc tgacggtgcc aggtaaacac cttcatccag catcatatgg aagaaacgct
    16501 taaaacgctc gacgtcacag gccatcacat cctgatagca cgtcacggac ccggcgtcgg
    16561 taaagaaaat accgaacatg ccgccaacgt ggtttaccac cagcggaatt ccggcttctt
    16621 ctgccgcttc cagcagacct tctgccagac gtgttgtcag ctcatccagc gtttcgtgaa
    16681 cgcccggctg cgcgacttca ttcagacagg cgaaaccggc tgccatcgca atcgggttac
    16741 cggaaagcgt acccgcctga tagaccggac ccgtcggggc cagcgcatcc attacatcac
    16801 gacgaccacc gaatgcgcct accggcattc caccgccgat gattttgccg aggcaggtta
    16861 aatctggcac tacgccgtaa taatcctgtg cgccagctag cgctacgcgg aaaccggtca
    16921 tcacttcatc gatgatcagc aacgcgccaa attcgtcaca cagcgcacgc agacctggca
    16981 ggaactctgg cagcggcgga acacagttca tattgcctgc caccggctcg acgataatac
    17041 aggcaatctc ttgcgggtat tgctcaaacg cggcgcgtac agaagccaga tcgttataag
    17101 tacaggttaa ggtatgtttg gcgaaatctg ccggaacgcc cggcgagttt ggctggccta
    17161 acgtgagtgc gccagaaccg gcttttacca gcaggcagtc agcgtgaccg tggtaacaac
    17221 cttcaaattt aataattttg tcgcgaccgg taaaaccacg ggccaggcgg atggcgctca
    17281 tggtcgcctc ggtgccggag ttcaccatgc gcaccatatc catggtcggg accagttcgg
    17341 tcaccagttg cgccattttc acttccattt cggtcggtgc accaaagctt aaaccacgct
    17401 cggcggcttc aatcacggca ttgcggatcg ccggatggtt atggcccagc accatcggcc
    17461 cccaggaacc gacataatcg atataggctt tgccatcaac atcgtacaga taagcgccgt
    17521 ccgctttttc gataaacagt ggagtgccgc ccacgccagt aaaggcgcga acaggggagt
    17581 tcacaccgcc agggatcagc tcgcgcgctg cgctgtaaag attttcagac ttactcatgg
    17641 agggttcctg attcgtagaa aaagtgaatg gctgctattc tatgttattc ataacaagtt
    17701 aaatactcgt caaacatcag gctgcttgta ctggtgcaat cctgatttct tagagtataa
    17761 aagttttgtg catttgaaac attacgcttt gcaaaggatt ttcatggaac gtgcgagtaa
    17821 aatgccgtca tcttatttgt atgaccaata agtgatcatt ggatgaaaac tgatactccc
    17881 tctttagaaa caccgcaggc cgcgcgcctg cgacgcagac aactgattcg ccaacttctt
    17941 gagcgcgata aaaccccgtt agccattttg tttatggcgg cagtcgtcgg cacgcttgtc
    18001 gggctggctg cggttgcttt tgacaaaggt gtcgcctggt tgcagaacca acgtatgggg
    18061 gcgctggtac atactgctga taattatccg cttctgttaa ccgtcgcttt tctctgttcg
    18121 gcggtgctgg cgatgttcgg ctacttttta gtgcgcaaat acgcgccgga agcaggtggt
    18181 tcggggatcc cggaaattga aggggcgctg gaagatcaac gtcccgttcg ctggtggcgt
    18241 gtattgccgg tgaaattctt tggcgggctg gggacactcg gcggaggcat ggtgctaggg
    18301 cgcgaagggc caaccgtgca gatcggcggt aatatcgggc ggatggtact cgatgttttc
    18361 cgcctgaaag gtgatgaagc tcgccatacg ctgttggcaa ccggtgcggc ggcgggtctg
    18421 gcggcggcct ttaacgcgcc gctggcgggt attttgttta ttatcgaaga gatgcgtccg
    18481 cagtttcgct atacgttaat ttcgattaaa gcggtattta ttggcgtcat tatgtcgacc
    18541 attatgtacc ggatttttaa tcatgaagtt gcgttgattg acgtcggtaa actttctgac
    18601 gcgccgctta atacgctgtg gctttatctg atcctcggta ttatttttgg cattttcggc
    18661 cctattttta ataaatgggt gctggggatg caggatttgc tgcaccgtgt gcacggcggc
    18721 aatattacca aatgggtact gatgggtggt gcgattggcg gtctgtgtgg attgctgggg
    18781 tttgttgcac cagcaacgtc gggcggcggt tttaacctga ttcctatcgc taccgcgggg
    18841 aatttcagca tgggaatgct ggtgtttatc ttcgtcgcgc gggtcattac caccttactc
    18901 tgcttctctt ccggcgcgcc gggcggtatt tttgccccga tgctggcgct gggtactgtg
    18961 ctgggaaccg ctttcggaat ggttgccgtt gagctgtttc cgcaatatca ccttgaggcg
    19021 gggacgtttg ctattgccgg aatgggggcg ttattggcgg catctattcg cgcgccgtta
    19081 acggggatca ttctggttct ggagatgacc gataactacc agctcatttt gccaatgatt
    19141 attaccggtc ttggcgcaac actattagcg caatttaccg gcgggaaacc gctatactcg
    19201 gcgattcttg cgcgcacgct ggcaaaacag gaagctgagc aactggcgcg aagcaaggcc
    19261 gcatcagcca gcgagaatac ttgaacgaaa taccagggta ttagataatg gcgattatta
    19321 ttgggttaga atttgcccaa ttgccgatgt cgtttggagc aaaatatgag tgatgacgta
    19381 gcactgccgc tggagtttac cgacgcagca gccaacaaag ttaaaagcct gatcgctgac
    19441 gaagataacc cgaatctgaa attacgcgtg tatatcaccg gtggcggttg cagcggcttc
    19501 cagtatggtt tcacctttga tgatcaggtg aacgaaggcg atatgaccat cgaaaaacag
    19561 ggcgttggcc tggtggttga tccgatgagc ctgcaatatc tggtcggcgg ttccgttgac
    19621 tataccgaag gactggaagg ttctcgtttc atcgtgacca acccgaacgc gaaaagcacc
    19681 tgcggttgcg gttcttcctt tagtatctaa tctgttgttc atgtgccgga taaactatcc
    19741 ggcacatctt ctctcttcaa cgcccattct catccagcgc aaacgtcggt agcttaagat
    19801 gccaacgaat agccgccagc cgaatcaata gcgtcacgac catgcccatc atactggctg
    19861 tttccagtgg tacggaaaat gtgtaataag ccgtagcgtg gacaataccg ccgataatac
    19921 aggcagttgc gtagatttct gtacgtaaaa tcatggggat ttcgcgggcc agaacatcac
    19981 gaatgatccc gccgccaacg ccagtaatga cgcccataca aaccgcgatt aacggaccgg
    20041 cttccgcatt aaaggcttta ttcacgccaa tgccgacaaa caccgccaga ccaacggcgt
    20101 ccagcaccgg caacatccat tttggtaagc gtcttggctg gcgcaccagc acgatggtca
    20161 gcatgctggt taccattgca acgaccagat cggtgggatc tttcacccaa aataccgggc
    20221 cgtgatccag cgccatgtcg cgaattgtcc cgccgcctac agcggtaacc acgcccagta
    20281 ccagaacacc aaaagggtcc atacgcaatt ttccggctaa caaaacgccg gagatggcaa
    20341 ataccgctgt gccgactata tccagccaat agacgagcat tgttcaatcc ccactgagca
    20401 cctaaaaagg tcaggcgcta atctacctgt gaaagcgcat tacagagctg ttgtgcagcg
    20461 aggataatac gtgggcttgc acgttcaaac cagtcactcg tgagaggaat aacgggaatt
    20521 ttgagctgtt caccccagta ttgttcgatt ttaggaattt ggtccggtcc gcctgtaatg
    20581 acaatcgcct gtggcgagcg tgctaacacc tgttcgcggc taacttgcgg ccagggaacc
    20641 cggctgtctt taaagatgtt ttctccgcca caaacttcga gtacctggtt ctgaatcgac
    20701 tcttttccac tggtaaatgg cggattaatg ccgaattgca gaaaaacacg ttttttaggt
    20761 ttatcagcat attgcgcttt caattgcgcg tactgatcca gcagggattg cgcggcttgt
    20821 tcggccttat ccggttgcgg actccagggg gccagttgac gtaacgcatt ggcaatttgt
    20881 tcaatgcttg tcgcatcgac ccacatcact tttattccca gcgaagccag ctggtcaacc
    20941 tgccgctcgg cattacctcc acgccaggca atcaccagat cgggtttcag cgcgacaatg
    21001 cgttccagat tcatcccctg ccaggtggaa acctgctcaa tcttttgcgc ttgtggagga
    21061 tagtcggaat agctgctgac cccaaccggc gtgatcccgg cggcaaaggc aagttcagtg
    21121 ttggcgggag aaagcgtgat gacgcgcggt gccgcgttga gccacagtgg cgcgagaaaa
    21181 gacagggcga ccagcgccct gaacagtgac ttagccatgt gccagtttct gcaccagtga
    21241 ctcaaccatc aggctggact gtttagcggc aacagccagg aactcatcga agctaagatg
    21301 agactgttga tcggccacgt cggagatggc gcgtacgaca acaaacggga cgttgaaatt
    21361 gtggcagaca tgggcgattg ccgtcgcttc catctctaca gcaatggcct gtgggaagtt
    21421 gtggcggatt ttcgccagac caacagaacc gttgatgaaa gcgtcgccgc taacaatcag
    21481 gccacgtaca gcgttaagat tcagttcggc aatgcaggcc tcagcggcag cgatcagttt
    21541 atcgtcagct ttaaagcctg ccggacagcc tggtaactga ccgtattcat aaccaaatgc
    21601 cgtgacatcc gcgtcgtgat aacgtgcttc gtccgagaca acgatatcgc ccactttcaa
    21661 cgttggtgcc aggccaccgg cagaaccggt gttaataatc acatctggct tgcagtgttc
    21721 caacagcaaa gtggcaccca gcgccgcagc gactttaccg atgcccgatt tcagaagcgc
    21781 aacctcggtt ccattcagtt ggccggtata gatttcgcaa ccgccgagac tgatagtttg
    21841 acggttttcg attttgtcac gcagcagcgt aacttcttct tccattgcac caatgatgcc
    21901 gattttcata gatttactcg cgataagccc gatttgaagg catagtttac catgcgctta
    21961 cggggaagcg tatttctcac gcgggagagg acatggcaca gattgatttc cgaaaaaaaa
    22021 taaactggca tcgtcgttac cgttcaccgc agggcgttaa aaccgaacat gagatcctgc
    22081 ggatcttcga gagcgatcgc gggcgtatca tcaactctcc ggcaattcgt cgtctgcaac
    22141 aaaagaccca ggtttttcca ctggagcgca atgccgccgt gcgcacgcgt cttacccact
    22201 cgatggaagt ccagcaggtg gggcgctaca tcgccaaaga aattttaagc cgtctgaaag
    22261 agcttaaatt gctggaagca tacggcctgg atgaactgac cggacctttt gaaagcattg
    22321 ttgagatgtc atgcctgatg cacgatatcg gcaatccgcc gtttggtcat tttggcgaag
    22381 cggcgataaa tgactggttt cgccagcgtt tatacccgga agatgccgaa agccagcctc
    22441 tgactgacga tcgctgcagc gtggcggcac tacgtttacg ggacggggaa gaaccgctta
    22501 acgagctgcg gcgcaagatt cgtcaggact tatgtcattt tgaggggaat gcacaaggca
    22561 ttcgtctggt gcatacattg atgcggatga atctcacctg ggcgcaggtt ggcggtattt
    22621 taaaatatac ccgtccagcg tggtggcgtg gcgaaacgcc tgagacacat cactatttaa
    22681 tgaaaaagcc aggttattat ctttctgaag aagcctatat tgcccggttg cgtaaagaac
    22741 ttaatttggc gctttacagt cgttttccat taacgtggat tatggaagct gccgacgaca
    22801 tctcctattg tgtggcagat cttgaagatg cggtagagaa aagaatattt accgttgagc
    22861 agctttatca tcatttgcac gaggcgtggg gccagcatga gaaaggttcg ctcttttcgc
    22921 tggtggttga aaatgcctgg gaaaaatcac gctcaaatag tttaagccgc agtacggaag
    22981 atcagttttt tatgtattta cgggtaaaca ccctaaataa actggtaccc tacgcggcac
    23041 aacgatttat tgataatctg cctgcgattt tcgccggaac gtttaatcat gcattattgg
    23101 aagatgccag cgaatgcagc gatcttctta agctatataa aaatgtcgct gtaaaacatg
    23161 tgtttagcca tccagatgtc gagcagcttg aattgcaggg ctatcgggtc attagcggat
    23221 tattagagat ttatcgccct ttattaagcc tgtcgttatc agactttact gaactggtag
    23281 aaaaagaacg ggtgaaacgt ttccctattg aatcgcgctt attccacaaa ctctcgacgc
    23341 gccatcggct ggcctatgtc gaagctgtca gtaaattacc gtcagattct cctgagtttc
    23401 cgctatggga atattattac cgttgccgcc tgctgcagga ttatatcagc ggtatgaccg
    23461 acctctatgc gtgggatgaa taccgacgtc tgatggccgt agaacaataa ccaggctttt
    23521 gtaaagacga acaataaatt tttacctttt gcagaaactt tagttcggaa cttcgcgcta
    23581 taaaacgaat ctgaagaaca cagcaatttt gcgttatctg ttaatcgaga ctgaaataca
    23641 tgaaaaaaac cacattagca ctgagtgcac tggctctgag tttaggtttg gcgttatctc
    23701 cgctctctgc aacggcggct gagacttctt cagcaacgac agcccagcag atgccaagcc
    23761 ttgcaccgat gctcgaaaag gtgatgcctt cagtggtcag cattaacgta gaaggtagca
    23821 caaccgttaa tacgccgcgt atgccgcgta atttccagca gttcttcggt gatgattctc
    23881 cgttctgcca ggaaggttct ccgttccaga gctctccgtt ctgccagggg ggccagggcg
    23941 gtaatggcgg cggccagcaa cagaaattca tggcgctggg ttccggcgtc atcattgatg
    24001 ccgataaagg ctatgtcgtc accaacaacc acgttgttga taacgcgacg gtcattaaag
    24061 ttcaactgag cgatggccgt aagttcgacg cgaagatggt tggcaaagat ccgcgctctg
    24121 atatcgcgct gatccagatc cagaacccga aaaacctgac cgcaattaag atggcggatt
    24181 ctgatgcact gcgcgtgggt gattacaccg tagcgattgg taacccgttt ggtttgggcg
    24241 agacggtaac ttccgggatt gtctctgcgt tggggcgtag cggcctgaat gccgaaaact
    24301 acgaaaactt catccagacc gatgcagcga tcaaccgtgg taactccggt ggtgcgctgg
    24361 ttaacctgaa cggcgaactg atcggtatca acaccgcgat cctcgcaccg gacggcggca
    24421 acatcggtat cggttttgct atcccgagta acatggtgaa aaacctgacc tcgcagatgg
    24481 tggaatacgg ccaggtgaaa cgcggtgagc tgggtattat ggggactgag ctgaactccg
    24541 aactggcgaa agcgatgaaa gttgacgccc agcgcggtgc tttcgtaagc caggttctgc
    24601 ctaactcctc cgctgcaaaa gcgggcatta aagcaggtga tgtgatcacc tcactgaacg
    24661 gtaagccaat cagcagcttt gccgcactgc gtgctcaagt gggtactatg ccggtgggca
    24721 gcaaactgac cctgggcttg ctgcgcgacg gtaagcaggt caacgtgaat ctggaactgc
    24781 agcagagcag ccagaatcag gttgattcca gctccatctt caacggcatt gaaggcgctg
    24841 agatgagcaa caaaggcaaa gatcagggcg tggtagtgaa caacgtgaaa acgggcactc
    24901 cggctgcgca gatcggcctg aagaaaggtg atgtgattat tggcgcgaac cagcaggcag
    24961 tgaaaaacat cgctgaactg cgtaaagttc tcgacagcaa accgtctgtg ctggcactga
    25021 acattcagcg cggcgacagc accatctacc tgttaatgca gtaatctccc tcaacccctt
    25081 cctgaaaacg ggaaggggtt ctccttacaa tctgtgaact tcaccacaac tccatacatc
    25141 ttcatcatcc tttaggcatt tgcacaatgc cgtacgttac gtacttcctt atgctaagcc
    25201 gtgcataacg gaggacttat ggctggctgg catcttgata ccaaaatggc gcaggatatc
    25261 gtggcacgta ccatgcgcat catcgatacc aatatcaacg taatggatgc ccgcgggcga
    25321 attatcggca gcggcgatcg tgagcgtatt ggtgaattgc acgaaggagc attgctggta
    25381 ctttcacagg gacgagtcgt cgatattgat gacgcggtgg cacgtcatct gcacggtgtg
    25441 cggcagggga ttaatctacc gttacggctg gaaggtgaaa ttgtcggcgt aattggcctg
    25501 acaggtgaac cagagaatct gcgtaaatat ggcgaactgg tctgcatgac ggctgaaatg
    25561 atgctggaac agtcgcggtt gatgcacttg ttggcgcagg atagccgttt gcgggaggaa
    25621 ctggtgatga acctgattca ggcagaggag aatactcccg cacttactga atgggcgcaa
    25681 cggctgggga tcgatctcaa tcaaccgcga gtggtggcta ttgttgaggt cgacagcggt
    25741 cagcttggtg tggacagcgc aatggcggag ttacaacaac tgcaaaacgc gctgactacg
    25801 cccgaacgta ataatctggt ggcgattgtc tcgctaaccg aaatggtggt gttgaaaccg
    25861 gcgttgaact cttttgggcg ctgggatgca gaagatcatc gtaagcgagt tgaacaactg
    25921 attacccgca tgaaagagta cggccagctg cgttttcgcg tttcactggg caactatttt
    25981 accggtcctg gcagtattgc ccgatcctat cgtacggcga aaacgacgat ggtggtgggt
    26041 aaacagcgga tgccagaaag tcgctgctat ttttatcagg atctgatgtt acctgtgtta
    26101 ctcgacagtt tgcgtggcga ctggcaggcc aacgaactgg cgcgaccgct ggcgcggctg
    26161 aaagcgatgg acaataacgg cttgctgcga cgaacgctgg cggcgtggtt tcgtcacaat
    26221 gtgcaaccgc tggcaacgtc aaaggcgttg tttattcatc gtaataccct ggagtatcgg
    26281 cttaatcgta tatcggaact gaccgggctt gatttgggca attttgatga caggttgctg
    26341 ctgtatgtgg cgttacaact ggatgaagag cggtaggtta tgcgttaagg ttgaggaggc
    26401 gctggctcat tcccctcagc ccgaagggga gaggggaatg cccgagttaa ccgtcaattc
    26461 gggattattt acgcgttagt ttttccagat cggcttcaat ctcgctgatc ttattggtta
    26521 cgactgactc caggtgacgt aagtcgtcga ggatcttacg cttaagatca acttcgctgc
    26581 ggtcacgctg acagatttga tcaagctcat caatgatata ccgtagattc gggctgattt
    26641 cctggacttc tttataaccc tgacccacac catcagcgac gaccgtttta cgctgacgcg
    26701 gatatttaaa cttaacgctc ttggcgaaaa actcgccttt gtctttctgg aaatagattt
    26761 tcagaatatc gttgttggct tcctgccgga ggctgtaacg atcaatttct tcaggattgg
    26821 taatacccag acttttcaga ttgtcgtaca tagcgttacc tcaaaatgag tcagtaaatt
    26881 gtgcttattt tagcatttgg cctggcccgc ccggctcgac tgtgatcgca gaaagccttt
    26941 ttcttccaga cttctatcat ggcgtaaaaa tcaaaaaatt acctgcttta ttgtggcgat
    27001 attccatggg atggattagc acatttatct tcgttttgcg aattacatga cagtccgaaa
    27061 caagatgaaa aagtatttaa aaaaggggga ggaagaatga gtaggacgaa ttattctgaa
    27121 ccagaaagca atcgggcagc gtcgtgctgc ccgtgtgcat acttttagtc gatggtacgc
    27181 aacagttcgt taatgccgac tttgccgcga gttttcgcgt caactttctt aacgataacc
    27241 gcacagtaaa gactgtattt gccatctttc gacggcagat tgcctgaaac aaccacagac
    27301 cccgccggaa cgcgtccgta gtggacttcg ccagtttcgc gatcatagat cttggtgctt
    27361 tgcccaatgt aaacacccat tgagatcacc gaaccttctt ctacgatgac gccttcaacg
    27421 atttctgaac gcgcgccgat gaagcagtta tcttcaataa tggtcgggtt agcctgcagc
    27481 ggttccagca caccgccgat gcccacgcca ccggaaaggt ggacgttttt accaatctgc
    27541 gcacaagaac cgacggtcgc ccaggtatcg accatggtgc cttcatcaac atatgcgccg
    27601 atgttgacgt aagacggcat cagcacggtg ttacgggcaa taaacgcacc ctgacgtacc
    27661 gccgcaggtg gtacaacgcg gaagccttct ttctggaaac gtgcttcgtc gtagtcggcg
    27721 aatttcatcg gcactttgtc gaagtagcgg ctttctgccc cttcgatcac ctgattatca
    27781 ttaatacgga aagagagcag caccgctttt ttcaaccact gatgcgtcac ccactgaccg
    27841 tcaatttttt ccgctacacg cagtgcgccg gaatccagca gggcgatcac ctgatttacc
    27901 gcttcgcggg taacggtgtc tgcattggct ggcgtgatct cggcacggcg ttcaaaagcg
    27961 gtttcaataa tgttctgtaa ctgctgcatt gttaaactct tttcatatca gtaaacacat
    28021 caccctttat cgtttggatt gagggcctct gtcaaccgct gatgcacttc ctgctgcaac
    28081 tcgttattaa gtgcacgccg gtcagcggtg gcaattatga ataaatcttc tactcgctcg
    28141 ccaatggttg taattcgggc accatgaagc gaaattccca gatcggcaaa aattttcccg
    28201 actcgcgcca gcagcccagg ttggtcgagg gcgatcagtt cgaggaacga tttgcggtcg
    28261 gtatgggttg gcaaaaacgt cacttcggtt tcaacagtaa aatggcgcaa tttggcgggt
    28321 tggcgacggg gctgcggtgg ctgccagcta ctttgtgtca gtacttgctc cagaccaaac
    28381 cgaataacct catggcgatc tgcggacagc gggctgccat cgggttccag cacgataaag
    28441 gtatccatcg ccataccgtc gcgagtggtg aatatttgtg cgtcgtgaac gcttaagttg
    28501 cgtctatcta actcggcaca aacagcagca aacaagtaag ggcggtccgg gctccagata
    28561 aaaatctcgg tgcctccacg cgtagcctgc gggctaagca ataccagcgg tttgcttaaa
    28621 tcatgctgta ataaatggcg cgcatgccag gccagttgat ttgggctatg gcggacaaaa
    28681 tagttagcac gacagcgtga ccaaatttgg tgcagcgcct cttcgtcgat gttatccatg
    28741 cgcagcagtg ccagcgcctg gagttggtga tggcgaaccc gttcgcgcat atccggcgtg
    28801 ttttgcattc cgcgtcgtag ctgtttttcg gtggcgaaat agagttcgcg caacagactt
    28861 tgcttccagc tattccacag cgtttcgttg gtggcgcaaa tgtcagccac tgtcaggcat
    28921 accagatagc gcagacgatt ttccgtttga acttcttcgg caaactgctt gatgacttcc
    28981 gggtcctgaa tatcgcgacg ctgagctgtc accgacatca gcaagtgttg gcgaaccagc
    29041 caggcgacca gctgtgtttc gcgtgagttc agcccgtgga gttcggcaaa atgcactaca
    29101 tcctgagcac cgagaatgga atggtcgccg ccgcgtcctt tggcgatatc gtgaaacagc
    29161 gcggcgatga aaatcagctc agttgacggt aggcgcggcc agacgtccac acacaacgga
    29221 tggcgctggc gcgtttcttc actggcaaaa ctctccagtt tcagcatcac gcggatagta
    29281 tgttcatcca ccgtgtaggc gtggaacaga tcaaactgca tctgcccgac gatatgtgac
    29341 cattgcggca tatacgcgcc gagtacgcta tggcgatgca ttggcaatag cccgcgccgc
    29401 accgctccgg ggtgacgcag aatgctcaaa aacagttttc gtgcttccgg aatattacac
    29461 agcggttgtt gcagatggcg acgggcatgg cgtaactggc gcagtgtggt ggagtaaatg
    29521 ccggtgatcg cactgttgcg caccatggtg tagaacatac gcaagatggc ttccggctgg
    29581 cgcataaata gtgtttcatc acgcaggtcg attagcgtac cgcgtagctg aaactcatcg
    29641 tcgattggac gtggtttttc gtcggcggga agggcgagga tggcttcatc gaacagttgc
    29701 agcagcatct ggttgagttc actgacgcgg cgtgtcacgc ggaagtaatc cttcatcatc
    29761 cgctcgaccg gttcgttacc ttcgccactg taattcagac gctgggcgac gctaagctgg
    29821 cgatcgaata acaggcgatt atcgtaacgg ctgacgacca gatgcagggc aaagcgaata
    29881 cgccacaata tatgcagaca ttcgtttaat tccgcccgct ccgctgaggt taagaagcca
    29941 aacccaacca tttcatccag cgatgttgcg ccaaaatgac ggcgggccac ccattgcaga
    30001 gtgtggatat cgcgcaagcc gccagggcta cttttgatgt ctggttcaag attgtagctg
    30061 gtgccgtggt aacgctgatg gcgctggttc tgttcttcaa ctttcgccgc gtagaacttg
    30121 tcggaaggcc agaatccttc gctgaaaata tgtttttgca gttcaagaaa tagcgcaaca
    30181 tcgccaatta ataagcggga ttcgatcaaa ttggtggcga cggttaaatc cgataaccct
    30241 tccagcatgc actcttcaag cgtgcgcaca ctatgaccga cttccagctt tacatcccag
    30301 agcagcgtta acagctcgcc cactttttgc gcctgatcgt ccgggagctt tttacggctt
    30361 aaaatcagta aatcgatgtc agaaagtgga tgcagctcgc cacgaccgta gccaccgacg
    30421 gcgaccaatg ccaggtcggc aatctggctg aatcccgctt caatccataa tcgttgcagg
    30481 agctggtcga taaactcggt gcgcgcctca atcaactgtt ctgcagagat cccattgtca
    30541 aaggcatcac ccagccaacg ctggaaagta tcgatatggg cttttatccc accgacggtt
    30601 aattcatcac ggggccagac gcatggattt tgcggttgac cgggcagggt ggggagagcg
    30661 gtgtttgcgt actgttctgg aagggtattc attgtgcgcc acccataaga ttaaattatc
    30721 gcattaaaaa agccggcatt cgccggcttc attttattcg tcgtgcgaga ttatcgccgg
    30781 gatggtgtca tccttgcgta gtgtcagaat ttcgcagccg ttatcagtca ccacaatagt
    30841 atgctcatat tgtgcagaca agctgcgatc tttggttttt accgtccagc catctttcat
    30901 ggtgcggatc tcttttttac ccgcgttgac cattggctcg atggtgaacg tcatcccagg
    30961 tttcagtacg acgttggttt cacgggagtc atagtgcagc acctgcggtt cttcgtggaa
    31021 gccgcgacca ataccgtgtc cgcaatattc acgaacgacg gagaagcctt ctgcttcgac
    31081 aaatttctga atcgccgcac cgatttcgcg cagattaatg cctggtttta ccatgcgtag
    31141 cgccaggtac aggctttctt gcgtgatacg gcacagacgt tcgcccatga tggtcggctt
    31201 accgacgata aacattttcg aggtatcgcc gtggaaacca tctttgatta cggtgacatc
    31261 aatgttaacg atatcgccat ctttcagcag cttcgcatcg tccgggatac cgtggcacac
    31321 cacttcatta attgagatgc aaacggattt cggataaccg tgatagccga ggcaggcaga
    31381 aaccgcgtgt tgttcattaa caatgtaatc attacagatg cgatccagct cgccggtgct
    31441 gacgcccggt ttaacatacg gttcgatcat ctccagcact tcggcagcca gtcggccagc
    31501 gacgcgcatt ttttcgatat cttctggggt cttgattgag atagccatta attctgtcca
    31561 tcagcgtcgg tgataccgac aatatatatg taagtgccgt caatggtatc acaccctggc
    31621 aaattgagaa tcattctgaa tttcgccaaa cgtgccactg aaggttttct ataatagaaa
    31681 attcgacgtc tgatgctgta cacagcgcca acaattattg gtgtccacgg cgtatttgtg
    31741 gtataaagcg cgccggactt ccgatccatt tcgtatacac agactggacg gaagcgacaa
    31801 tctcactttg tgtaacaaca cacacgtatc ggcacatatt ccggggtgcc ctttggggtc
    31861 ggtaatatgg gatacgtgga ggcataaccc caacttttat atagaggttt taatcatggc
    31921 aactgtttcc atgcgcgaca tgctcaaggc tggtgttcac ttcggtcacc agacccgtta
    31981 ctggaacccg aaaatgaagc cgttcatctt cggtgcgcgt aacaaagttc acatcatcaa
    32041 ccttgagaaa actgtaccga tgttcaacga agctctggct gaactgaaca agattgcttc
    32101 tcgcaaaggt aaaatccttt tcgttggtac taaacgcgct gcaagcgaag cggtgaaaga
    32161 cgctgctctg agctgcgacc agttcttcgt gaaccatcgc tggctgggcg gtatgctgac
    32221 taactggaaa accgttcgtc agtccatcaa acgtctgaaa gacctggaaa ctcagtctca
    32281 ggacggtact ttcgacaagc tgaccaagaa agaagcgctg atgcgcactc gtgagctgga
    32341 gaaactggaa aacagcctgg gcggtatcaa agacatgggc ggtctgccgg acgctctgtt
    32401 tgtaatcgat gctgaccacg aacacattgc tatcaaagaa gcaaacaacc tgggtattcc
    32461 ggtatttgct atcgttgata ccaactctga tccggacggt gttgacttcg ttatcccggg
    32521 taacgacgac gcaatccgtg ctgtgaccct gtacctgggc gctgttgctg caaccgtacg
    32581 tgaaggccgt tctcaggatc tggcttccca ggcggaagaa agcttcgtag aagctgagta
    32641 ataaggcacg ctcgtacgag cccttataga ccaggtagta cacgtttggt tagggggcct
    32701 gcatatggcc ccctttttca cttttatatc tgtgcggttc aatgccgggc agatcacatc
    32761 tccgaggatt ttagaatggc tgaaattacc gcatccctgg taaaagagct gcgtgagcgt
    32821 actggcgcag gcatgatgga ttgcaaaaaa gcactgactg aagctaacgg cgacatcgag
    32881 ctggcaatcg aaaacatgcg taagtccggt gctattaaag cagcgaaaaa agcaggcaac
    32941 gttgctgctg acggcgtgat caaaaccaaa atcgacggca actacggcat cattctggaa
    33001 gttaactgcc agactgactt cgttgcaaaa gactctggtt tccaggcgtt cgcagacaaa
    33061 gttctggacg cagctgttgc tggcaaaatc actgacgttg aagttctgaa agcacagttc
    33121 gaagaagaac gtgttgcgct ggtagcgaaa attggtgaaa acatcaacat tcgccgcgtt
    33181 gctgcgctgg aaggcgacgt tctgggttct tatcagcacg gtgcgcgtat cggcgttctg
    33241 gttgctgcta aaggcgctga cgaagagctg gttaaacaca tcgctatgca cgttgctgca
    33301 agcaagccag aattcatcaa accggaagac gtatccgctg aagtggtaga gaaagaatac
    33361 caggtacagc tggatatcgc gatgcagtct ggtaagccga aagaaatcgc agagaaaatg
    33421 gttgaaggcc gcatgaagaa attcaccggc gaagtttctc tgaccggtca gccgttcgtt
    33481 atggaaccaa gcaaaactgt tggtcagctg ctgaaagagc ataacgctga agtgactggc
    33541 ttcatccgct tcgaagtagg tgaaggcatc gagaaagttg agactgactt tgcagcagaa
    33601 gttgctgcga tgtccaagca gtcttaatta tcgaaaagga gccgcctgag ggcggcttct
    33661 ttttgtgccc atcttgtaaa ttcagctaac ccttgtgggg ctgcgctgaa aagcgacgta
    33721 caatgtcgct agtattaatt catttcaatc gttgacagtc tcaggaaaga aacatggcta
    33781 ccaatgcaaa acccgtctat aaacgcattc tgcttaagtt gagtggcgaa gctctgcagg
    33841 gcactgaagg cttcggtatt gatgcaagca tactggatcg catggctcag gaaatcaaag
    33901 aactggttga actgggtatt caggttggtg tggtgattgg tgggggtaac ctgttccgtg
    33961 gcgctggtct ggcgaaagcg ggtatgaacc gcgttgtggg cgaccacatg gggatgctgg
    34021 cgaccgtaat gaacggcctg gcaatgcgtg atgcactgca ccgcgcctat gtgaacgctc
    34081 gcctgatgtc cgctattcca ttgaatggcg tgtgcgacag ctacagctgg gcagaagcta
    34141 tcagcctgtt gcgcaacaac cgtgtggtga tcctctccgc cggtacaggt aacccgttct
    34201 ttaccaccga ctcagcagct tgcctgcgtg gtatcgaaat tgaagccgat gtggtgctga
    34261 aagcaaccaa agttgacggc gtgtttactg ctgatccggc gaaagatcca actgcaacca
    34321 tgtacgagca attgacttac agcgaagtgc tggaaaaaga gctgaaagtc atggacctgg
    34381 cggccttcac gctggctcgt gaccataaat taccgattcg tgttttcaac atgaacaaac
    34441 cgggtgcgct gcgccgtgtg gtaatgggtg aaaaagaagg gactttaatc acggaataat
    34501 tcccgtgatg gataaataag ggtaagattc cgcgtaagta tcgcggggga tcttacccgt
    34561 aatatgttta atcagggcta tacttagcac acttccactg tgtgtgactg tctggtctga
    34621 ctgagacaag ttttcaagga ttcgtaacgt gattagcgat atcagaaaag atgctgaagt
    34681 acgcatggac aaatgcgtag aagcgttcaa aacccaaatc agcaaaatac gcacgggtcg
    34741 tgcttctccc agcctgctgg atggcattgt cgtggaatat tacggcacgc cgacgccgct
    34801 gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact ctgaaaatca acgtgtttga
    34861 tcgttcaatg tctccggccg ttgaaaaagc gattatggcg tccgatcttg gcctgaaccc
    34921 gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg ctgacggaag aacgtcgtaa
    34981 agatctgacc aaaatcgttc gtggtgaagc agaacaagcg cgtgttgcag tacgtaacgt
    35041 gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa gataaagaga tcagcgaaga
    35101 cgacgatcgc cgttctcagg acgatgtaca gaaactgact gatgctgcaa tcaagaaaat
    35161 tgaagcggcg ctggcagaca aagaagcaga actgatgcag ttctgatttc ttgaacgaca
    35221 aaaacgccgc tcagtagatc cttgcggatc ggctggcggc gttttgcttt ttattctgtc
    35281 tcaactctgg atgtttcatg aagcaactca ccattctggg ctcgaccggc tcgattggtt
    35341 gcagcacgct ggacgtggtg cgccataatc ccgaacactt ccgcgtagtt gcgctggtgg
    35401 caggcaaaaa tgtcactcgc atggtagaac agtgcctgga attctctccc cgctatgccg
    35461 taatggacga tgaagcgagt gcgaaacttc ttaaaacgat gctacagcaa cagggtagcc
    35521 gcaccgaagt cttaagtggg caacaagccg cttgcgatat ggcagcgctt gaggatgttg
    35581 atcaggtgat ggcagccatt gttggcgctg ctgggctgtt acctacgctt gctgcgatcc
    35641 gcgcgggtaa aaccattttg ctggccaata aagaatcact ggttacctgc ggacgtctgt
    35701 ttatggacgc cgtaaagcag agcaaagcgc aattgttacc ggtcgatagc gaacataacg
    35761 ccatttttca gagtttaccg caacctatcc agcataatct gggatacgct gaccttgagc
    35821 aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg ccctttccgt gagacgccat
    35881 tgcgcgattt ggcaacaatg acgcccgatc aagcctgccg tcatccgaac tggtcgatgg
    35941 gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa aggtctggaa tacattgaag
    36001 cgcgttggct gtttaacgcc agcgccagcc agatggaagt gctgattcac ccgcagtcag
    36061 tgattcactc aatggtgcgc tatcaggacg gcagtgttct ggcgcagctg ggggaaccgg
    36121 atatgcgtac gccaattgcc cacaccatgg catggccgaa tcgcgtgaac tctggcgtga
    36181 agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc cgcaccggat tatgatcgtt
    36241 atccatgcct gaaactggcg atggaggcgt tcgaacaagg ccaggcagcg acgacagcat
    36301 tgaatgccgc aaacgaaatc accgttgctg cttttcttgc gcaacaaatc cgctttacgg
    36361 atatcgctgc gttgaattta tccgtactgg aaaaaatgga tatgcgcgaa ccacaatgtg
    36421 tggacgatgt gttatctgtt gatgcgaacg cgcgtgaagt cgccagaaaa gaggtgatgc
    36481 gtctcgcaag ctgaggataa tccggctaca gagagtcgcg ctatttgtta gcgtagggct
    36541 tcagtgatat agtctgcgcc atctgatcgt aagtagttgg ctttataagg tcaggtatgc
    36601 cgtggtttta cacggctttt ttttgtatag gcttcagtat tcctgagtac cgtaaaccct
    36661 gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa ccacttagcg aaaaattgcc
    36721 agcgcatggc tgccgtcatg ttgcgatcat tatggacggc aatggtcgct gggcaaaaaa
    36781 gcaagggaag attcgtgcct ttgggcataa agccggggca aaatccgtcc gccgggctgt
    36841 ctcttttgcg gccaacaacg gtattgaggc gttaacgctg tatgccttta gtagtgaaaa
    36901 ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa ctgtttgtgt gggcgctcga
    36961 tagcgaagta aaaagtctgc accgacataa cgtgcgtctg cgtattattg gcgataccag
    37021 tcgctttaac tcgcgtttgc aagaacgtat tcgtaaatct gaagcgctaa cagccgggaa
    37081 taccggtctg acgctgaata ttgcggcgaa ctacggtgga cgttgggata tagtccaggg
    37141 agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg caaccagatc agatagatga
    37201 agagatgcta aaccagcatg tctgtatgca tgaactggcc cctgtagatt tagtaattag
    37261 gactgggggg gagcatcgca ttagtaactt tttgctttgg caaattgcct atgccgaact
    37321 ttactttaca gatgttctct ggcccgattt cgatgaacaa gactttgaag gggcgttaaa
    37381 tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag cccggtgatg aaacagcctg
    37441 atgggggtcg cttttgctga agtatcgcct gatatctgct tttgtgttaa tacccgtcgt
    37501 catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc attgtaacgc tggtggtctg
    37561 catgctggca gcgtgggaat ggggacagct tagcggtttt accactcgtt cgcagcgagt
    37621 atggttggcg gtgttatgcg ggttattgtt ggcgctgatg ctttttctgt tgccggaata
    37681 tcaccgaaat attcatcaac cgctggttga aatctcactt tgggcttcgc tgggttggtg
    37741 gattgtcgcg ctattgctgg tgctgtttta cccaggttcc gcagcaatct ggcgtaactc
    37801 taaaacattg cgccttattt ttggcgtgct aaccattgtt cccttcttct ggggcatgct
    37861 ggcgttacgg gcctggcact acgacgagaa tcattacagt ggcgcaatat ggctgctcta
    37921 tgtcatgatc ctggtatggg gcgctgactc tggcgcatat atgtttggca aattgtttgg
    37981 taaacataag ctggcaccga aggtttctcc gggtaaaacc tggcaaggct ttataggtgg
    38041 actcgcgacc gcagcagtaa tctcatgggg ttatggcatg tgggcgaatc tcgacgtcgc
    38101 tcccgtcacc ttactcattt gctctattgt cgcagcgtta gcctcagtgc tcggcgatct
    38161 gaccgagagt atgtttaagc gtgaagcagg aattaaggac agcggtcatt taattccagg
    38221 acacggtggt attttagatc gtattgatag cctgacggct gcggtaccgg tctttgcttg
    38281 cttgttgtta ctggtattca ggacgcttta acggaaggta atatgctgag ttttctctgg
    38341 gatttggctt cgttcatcgt tgcactgggt gtacttatca ccgtgcatga atttggtcat
    38401 ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt tctcaatagg gtttggtaag
    38461 gcgctctggc ggcgaactga taagctcggc accgaatatg ttatcgccct gatcccgttg
    38521 ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg tcgttccgga actccgccac
    38581 catgccttca ataataaatc tgtcggccaa cgagcggcga ttattgccgc aggtccggtt
    38641 gcaaacttca tttttgctat ctttgcctac tggctggttt ttattattgg tgtgcctggc
    38701 gtacgtccgg tggttggcga aatagcagcc aattcgatag ctgcggaagc acaaattgca
    38761 ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc ctgattggga tgccgtgcgt
    38821 ttgcagttgg tcgataaaat tggcgatgaa agcaccacca ttacagtagc gccatttggc
    38881 agcgaccaac ggcgggatgt aaagctcgat ttacgtcact gggcgtttga gcctgataaa
    38941 gaagatccgg tatcttcgct ggggattcgt cctcgtgggc cgcaaattga acctgtactg
    39001 gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt tgcaagcagg cgacaggatc
    39061 gttaaagtcg atggtcagcc cttaacgcag tgggtgacct ttgtgatgct tgtccgggat
    39121 aacccgggta aatccttagc gttagaaatc gaaaggcagg ggagtccctt gtctttgaca
    39181 ttaatcccgg agagtaaacc gggtaatggt aaagcgattg gttttgtcgg tattgagccg
    39241 aaagtcattc ctttgccaga tgagtataaa gttgtacgcc agtatgggcc gttcaacgcc
    39301 atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc tgacggtcag tatgctggga
    39361 aaattgatca ccggtgatgt gaaactgaac aacctcagtg ggccgatctc tatcgccaag
    39421 ggggctggga tgacagcgga actcggggta gtttattacc tgccgtttct tgcgcttatt
    39481 agcgtgaact tagggataat taacctgttt ccgttgcccg tacttgacgg ggggcatctg
    39541 ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat ccgagcgggt tcaagacttt
    39601 tgttatcgca ttggctcgat tctgctggtg ctgttaatgg ggcttgcact tttcaatgat
    39661 ttctctcggt tatgagagtt agttaggaag aacgcataat aacgatggcg atgaaaaagt
    39721 tgctcatagc gtcgctgctg tttagcagcg ccaccgtata cggtgctgaa gggttcgtag
    39781 tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt tggtgcggcc ctcctcagta
    39841 tgccggtgcg cacaggcgac acggttaatg atgaagatat cagtaatacc attcgcgctc
    39901 tgtttgctac cggcaacttt gaggatgttc gcgtccttcg tgatggtgat acccttctgg
    39961 ttcaggtaaa agaacgtccg accattgcca gcattacttt ctccggtaac aaatcggtga
    40021 aagatgacat gctgaagcaa aacctcgagg cttctggtgt gcgtgtgggc gaatccctcg
    40081 atcgcaccac cattgccgat atcgagaaag gtctggaaga cttctactac agcgtcggta
    40141 aatatagcgc cagcgtaaaa gctgtcgtga ccccgctgcc gcgcaaccgt gttgacctaa
    40201 aactggtgtt ccaggaaggt gtgtcagctg aaatccagca aattaacatt gttggtaacc
    40261 atgctttcac caccgacgaa ctgatctctc atttccaact gcgtgacgaa gtgccgtggt
    40321 ggaacgtggt aggcgatcgt aaataccaga aacagaaact ggcgggcgac cttgaaaccc
    40381 tgcgtagtta ctatctggat cgcggttatg cccgtttcaa catcgactct acccaggtca
    40441 gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa catcaccgaa ggcgatcagt
    40501 acaagctttc tggcgttcaa gtgagcggca accttgccgg gcactccgct gaaattgagc
    40561 agctgactaa gatcgagccg ggcgagctgt ataacggcac caaagtgacc aagatggaag
    40621 atgacatcaa aaagcttctc ggtcgctatg gttatgccta tccgcgcgta cagtcgatgc
    40681 ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt gaacgttgat gcgggtaacc
    40741 gtttctacgt gcgtaagatc cgttttgaag gtaacgatac ctcgaaagat gccgtcctgc
    40801 gtcgcgaaat gcgtcagatg gaaggtgcat ggctgggaag cgatctggtc gatcagggta
    40861 aggagcgtct gaatcgtctg ggcttctttg aaactgtcga taccgatacc caacgtgttc
    40921 cgggtagccc ggaccaggta gatgtagtct acaaggtgaa agaacgtaac accggtagct
    40981 tcaacttcgg gattggttac ggtactgaaa gtggcgtgag cttccaggct ggcgtgcaac
    41041 aggataactg gttaggtaca ggttatgctg ttggtatcaa cgggaccaaa aacgattacc
    41101 agacctatgc tgaattgtcg gtaaccaacc cgtacttcac tgtagatggt gtaagcctcg
    41161 gtggtcgtct cttctataat gacttccagg cagatgacgc cgacctgtcc gactatacca
    41221 acaagagtta tggtacagac gtgacgttgg gcttcccgat caacgaatat aactcgctgc
    41281 gtgcaggtct gggttatgta cataactccc tgtccaacat gcagcctcag gttgcgatgt
    41341 ggcgttatct gtactctatg ggtgaacatc cgagcacctc tgatcaggat aacagcttca
    41401 aaacggacga cttcacgttc aactatggtt ggacctataa caagcttgac cgtggttact
    41461 tcccgacaga tggttcacgt gtcaacctga ccggtaaagt gaccattcct ggatcggata
    41521 acgaatacta caaagtgacg ttagacacgg cgacttatgt gccgatcgat gacgatcaca
    41581 aatgggttgt tctggggcgt acccgctggg gttatggtga tggtttaggc ggcaaagaga
    41641 tgccgttcta cgagaacttc tatgccggtg gttccagcac cgtgcgtggc ttccagtcca
    41701 ataccattgg tccgaaagca gtttacttcc cgcatcaggc cagtaattat gatccggact
    41761 atgattacga atgtgcgact caggacggcg cgaaagacct gtgtaaatcg gatgatgctg
    41821 taggcggtaa cgccatggcg gttgccagcc tcgagttcat caccccgacg ccgtttatta
    41881 gcgataagta tgctaactcg gttcgtactt ccttcttctg ggatatgggt accgtttggg
    41941 atacaaactg ggattccagc caatattctg gatatccgga ctatagtgat ccaagcaata
    42001 tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc attggggccg ttggtgttct
    42061 cctacgccca gccgttcaaa aagtacgatg gagacaaggc agaacagttc cagtttaaca
    42121 tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag tggtagtgta gcgatgactt
    42181 taggcgatca atataagatc gccgggccac gcaaagaact gcaccctccg gtgcaaatgg
    42241 gatggtaagg agtttattgt gaaaaagtgg ttattagctg caggtctcgg tttagcactg
    42301 gcaacatctg ctcaggcggc tgacaaaatt gcaatcgtca acatgggcag cctgttccag
    42361 caggtagcgc agaaaaccgg tgtttctaac acgctggaaa atgagttcaa aggccgtgcc
    42421 agcgaactgc agcgtatgga aaccgatctg caggctaaaa tgaaaaagct gcagtccatg
    42481 aaagcgggca gcgatcgcac taagctggaa aaagacgtga tggctcagcg ccagactttt
    42541 gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc gttccaacga agaacgcggc
    42601 aaactggtta ctcgtatcca gactgctgtg aaatccgttg ccaacagcca ggatatcgat
    42661 ctggttgttg atgcaaacgc cgttgcttac aacagcagcg atgtaaaaga catcactgcc
    42721 gacgtactga aacaggttaa ataagtaatg ccttcaattc gactggctga tttagcgcag
    42781 cagttggatg cagaactaca cggtgatggc gatatcgtca tcaccggcgt tgcgtccatg
    42841 caatctgcac aaacaggtca cattacgttc atggttaacc caaaataccg tgagcattta
    42901 ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg atcttccttt cgcgaaaagt
    42961 gccgcgctgg tagtgaagaa tccctacctg acttacgcgc gcatggcgca aattttagat
    43021 accacgccgc agcccgcgca gaacattgca cccagtgcgg tgatcgacgc gacggcgaag
    43081 ctgggtaaca acgtatcgat tggcgctaac gcggtgattg agtccggcgt tgaactgggc
    43141 gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa acagcaaaat cggtgcaggt
    43201 tcgcgtctct gggcgaacgt aaccatttac catgagatcc agatcggtca gaattgcctg
    43261 atccagtccg gaacagtggt aggcgcagac ggctttggtt atgccaacga tcgtggtaac
    43321 tgggtgaaga tcccacagat tggtcgcgta attattggcg atcgcgtgga gatcggtgcc
    43381 tgcacaacca tcgatcgcgg cgcgctggat gacactatta ttggcaatgg cgtgatcatt
    43441 gataaccagt gccagattgc acataacgtc gtgattggcg acaatacggc ggttgccggt
    43501 ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact gcatgatcgg cggagccagc
    43561 gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg ttacgggcat gggtatggtg
    43621 atgcgtccca tcactgaacc aggcgtctat tcctcaggca ttccgctgca acccaacaaa
    43681 gtctggcgca aaaccgctgc actggtgatg aacattgatg acatgagcaa gcgtctgaaa
    43741 tcgcttgagc gcaaggttaa tcaacaagac taacgttcca tcttttgttc gccaaacttt
    43801 acggcctgtc tcattcttac gattgcggca ggccgtgtta ttattgtcgt ttcttatatt
    43861 ttgacaggaa gagtatcttg actactaaca ctcatactct gcagattgaa gagattttag
    43921 aacttctgcc gcaccgtttc cctttcttac tggtggatcg cgtgctggat tttgaagaag
    43981 gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga gccattcttc cagggccatt
    44041 tccctggaaa accgattttc ccgggtgtgc tgattctgga agcaatggca caggcaacag
    44101 gtattctggc gtttaaaagc gtaggaaaac tggaaccggg tgagctgtac tatttcgctg
    44161 gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg cgatcaaatg atcatggaag
    44221 tcactttcga aaaaacgcgc cgcggcctga cccgttttaa aggggttgct ctggtcgacg
    44281 gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag ccgggaggcc tgatacgtga
    44341 ttgataaatc cgcctttgtg catccaaccg ccattgtgga agagggcgcg tcgattggcg
    44401 cgaacgcaca cattggtcct ttttgtatcg ttggacccca tgtcgaaatt ggtgagggta
    44461 ccgtactgaa atctcacgtt gtcgtgaatg gccatactaa aattggccgc gataatgaga
    44521 tttatcagtt cgcctccatc ggtgaagtta accaggatct gaaatatgct ggcgaaccga
    44581 cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag cgtcaccatt catcgtggca
    44641 cagtccaggg cggtggattg acgaaggtgg gcagcgacaa cttactgatg atcaacgcgc
    44701 acattgcgca cgattgtacg gtaggtaacc gctgtattct cgccaacaac gcaacgctgg
    44761 cgggtcacgt atcggtagac gacttcgcga tcatcggcgg catgaccgca gtccatcagt
    44821 tctgcatcat tggtgcgcat gtgatggttg gcggttgctc cggtgtggcg caggacgtcc
    44881 ctccttatgt cattgcgcag ggtaaccacg caacgccgtt cggtgtcaat atcgaagggc
    44941 tgaagcgccg cggattcagc cgcgaggcga ttaccgctat ccgcaatgcg tataagctga
    45001 tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga aattgctgaa ctggcggaaa
    45061 cgtatccgga agtgaaagct tttaccgatt tctttgcacg ctcaacgcgc ggtctgattc
    45121 gttaatgact gaacagcgtc cattaacgat tgccctggtc gccggagaaa cctccggcga
    45181 tatcctgggg gccggtttaa tccgcgctct gaaagaacgt gtgcccaacg cccgctttgt
    45241 tggcgttgcc gggccacgaa tgcaggctga aggctgcgaa gcctggtacg aaatggaaga
    45301 gttggcggtg atgggcattg ttgaagtgct cggtcgtctg cgtcgcttac tgcatattcg
    45361 tgccgatctg acaaagcgtt ttggtgaact aaagccagat gtttttgttg gcattgatgc
    45421 gcctgacttc aatattaccc tcgaaggtaa ccttaaaaag cagggtatca aaaccattca
    45481 ttatgtcagt ccgtccgtct gggcgtggcg acagaaacgc gttttcaaaa taggcagagc
    45541 caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg ttttatgaca agtataacgt
    45601 gccgtgccgt tttatcggtc acacgatggc tgatgccatg ccgttagatc cagataaaaa
    45661 cgccgcccgt gatgtgctgg gtatccctca cgatgcccac tgtctggcat tgttgccggg
    45721 cagccgtggt gcggaagtcg agatgctgag cgccgatttc ctgaaaacgg cccagctttt
    45781 gcgccagacg taccccgatc tggagatcgt ggtgccgctg gtgaatgcca aacgccgcga
    45841 gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctt tcagttcatt tgctggatgg
    45901 gatgggccgt gaggcgatgg tcgccagcga tgcggcacta ctggcgtcgg ggacggcagc
    45961 cctggagtgt atgctggcga aatgcccgat ggtggtggga tatcgcatga agccttttac
    46021 cttctggttg gcgaagcggc tggtgaaaac tgaatatgtc tcgctgccaa atctgctggc
    46081 gggcagagag ttagtcaaag aattattgca ggaagagtgt gagccgcaaa aactggctgc
    46141 ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac gcaatgcacg ataccttccg
    46201 tgaactgcat cagcagatcc gctgcaatgc cgatgagcag gcggcacaag ccgttctgga
    46261 gttagcacaa tgatcgaatt tgtctatccg cacacgcagc tggttgcggg tgtggatgaa
    46321 gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg cggtgatcct tgacccggcg
    46381 cgcccgattg ccgggctgaa tgattccaaa aagctgagcg aaaaacgccg tctggcgctc
    46441 tatgaagaga tcaaagagaa agcgttgagc tggagtctgg gccgcgcgga accccacgaa
    46501 atcgacgagc tgaacattct tcatgcgacc atgctggcga tgcagcgtgc cgtcgctggg
    46561 ctgcatattg cgccggaata tgtgttgatt gatggtaacc gctgcccgaa attaccgatg
    46621 cctgcgatgg ctgtggtgaa aggcgatagc cgcgtaccgg aaatcagtgc cgcgtctatc
    46681 ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg atattgtttt cccgcaatat
    46741 ggttttgccc aacacaaagg gtatccaacc gcttttcatc tggaaaaact ggctgaatat
    46801 ggcgcgactg aacatcatcg tcgcagcttt gggcctgtca aacgagcact gggacttgcg
    46861 tcctgattct tgtgtcgaga ttaagtaaac cggaatctga agatgtctga accacgtttc
    46921 gtacacctgc gggtgcacag cgactactcg atgatcgatg gcctggccaa aaccgcaccg
    46981 ctggtaaaaa aggcggcggc gttgggtatg ccagcactgg cgatcaccga tttcaccaac
    47041 ctttgcggtc tggtgaagtt ctacggagcg ggacatggcg cagggattaa gcctatcgtc
    47101 ggggcagatt ttaacgtcca gtgcgacctg ttgggtgatg agttaaccca cctgacggta
    47161 ctggcggcga acaataccgg ctatcagaat ctgacgttgc tgatctcaaa agcgtatcag
    47221 cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact ggcttatcga attaaacgaa
    47281 gggttgatcc ttctttccgg cgggcgcatg ggcgacgtcg gacgcagtct tttgcgtggt
    47341 aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag aacacttccc ggatcgctat
    47401 tttctcgagc tgatccgcac cggcaggccg gatgaagaaa gctatctgca cgcggcggtg
    47461 gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacta acgatgtgcg ctttatcgac
    47521 agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc acgacggctt taccctcgac
    47581 gatcctaaac gcccgcgtaa ctattcgccg cagcaatata tgcgtagcga agaggagatg
    47641 tgcgagctgt ttgccgacat ccccgaagct cttgccaaca ccgttgagat cgccaaacgc
    47701 tgtaacgtaa ctgtgcgtct tggtgaatac ttcctgccgc agttcccgac cggggacatg
    47761 agcaccgaag attatctggt caagcgtgca aaagagggcc tggaagagcg tctggccttt
    47821 ttattccctg atgaggaaga acgtgttaag cgccgcccgg aatatgacga acgtctggag
    47881 actgaacttc aggttatcaa ccagatgggc ttcccgggct acttccttat cgttatggaa
    47941 tttatccagt ggtcgaaaga taacggtgta ccggtagggc caggccgtgg ctccggcgca
    48001 ggctcactgg tggcctacgc gctgaaaatc accgacctcg atccgctgga atttgacctg
    48061 ctgttcgaac gtttccttaa cccggaacgt gtctccatgc ctgacttcga cgttgacttc
    48121 tgtatggaga aacgcgatca ggttatcgaa cacgtagcgg acatgtacgg tcgtgatgcg
    48181 gtatcgcaga ttatcacctt cggtacgatg gcggcgaaag cggtgatccg cgacgtaggc
    48241 cgcgtgctgg ggcatccgta cggctttgtc gatcgtatct cgaaactgat cccgcccgat
    48301 ccggggatga cgctggcgaa agcgtttgaa gccgagccgc agctgccgga aatctacgaa
    48361 gcggatgaag aagttaaggc gctgatcgac atggcgcgca aactggaagg ggtcacccgt
    48421 aacgccggta agcacgccgg tggggtggtt atcgcgccga ccaaaattac cgattttgcg
    48481 ccgctttatt gcgatgaaga gggcaaacat ccggtcaccc agtttgataa aagcgacgtt
    48541 gaatacgccg gactggtgaa gttcgacttc cttggtttgc gtacgctcac catcatcaac
    48601 tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg gcgagccgcc gctggatatc
    48661 gccgcgatcc cgctggatga caagaaaagc ttcgacatgc tgcaacgctc ggaaaccacg
    48721 gcggtattcc agcttgaatc gcgcggcatg aaggacctga tcaagcgtct gcaacctgac
    48781 tgcttcgaag atatgatcgc actggtggca ctgttccgcc ctggtccgtt gcagtcaggg
    48841 atggtggata actttattga ccgtaaacat ggtcgcgaag aaatctccta tccagacgta
    48901 cagtggcagc atgaaagcct gaaaccggta ctggagccaa cctacggcat tatcctgtat
    48961 caggaacagg tcatgcagat tgcgcaggtg ctttctggtt ataccctcgg tggcgcggat
    49021 atgctgcgtc gtgcgatggg taagaaaaag ccggaagaga tggcgaagca gcgttctgta
    49081 tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac tggcgatgaa aatcttcgac
    49141 ctggtggaga aattcgcggg ttacggattt aacaaatcgc actctgcggc ctatgctttg
    49201 gtgtcatatc aaacattatg gctgaaagcg cactatccgg cagagtttat ggcggcggta
    49261 atgaccgccg atatggacaa caccgagaag gtggtgggcc tggtggatga gtgctggcgg
    49321 atggggctga aaatcctgcc accagatata aactccggtc tttaccattt ccacgtcaac
    49381 gacgacggcg aaatcgtgta tggtattggc gcgatcaaag gggtaggtga aggtccgatt
    49441 gaggcgatca tcgaagcccg taataaaggc ggctacttcc gcgaactgtt tgatctctgc
    49501 gcccgaaccg acaccaaaaa gttaaaccgg cgggtgttgg aaaaactgat tatgtccggg
    49561 gcgtttgacc gtcttgggcc acaccgcgca gcgctgatga actcgctggg cgatgcgtta
    49621 aaagcggcag atcaacacgc gaaagcggaa gccatcggtc aggccgatat gttcggcgtg
    49681 ctggccgaag agccggaaca aattgaacaa tcctacgcca gctgccaacc gtggccggag
    49741 caggtggtat tagatgggga acgtgaaacg ttaggtctgt acctgacggg acaccctatc
    49801 aaccagtatt tgaaagagat tgagcgttat gtcggaggcg taaggctgaa agacatgcac
    49861 ccgacagaac gtggtaaagt catcacggct gcggggctcg ttgttgccgc gcgggttatg
    49921 gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg atgaccgttc cgggcggctg
    49981 gaagtgatgt tgtttactga cgccctggat aaataccagc aattgctgga aaaagaccgc
    50041 atacttatcg tcagcggaca ggtcagcttt gatgacttca gcggtgggct taaaatgacc
    50101 gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat atgctcgcgg gcttgctatc
    50161 tcgctgacgg acaggcaaat tgatgaccag cttttaaacc gactccgtca gtctctggaa
    50221 ccccaccgct ctgggacaat tccagtacat ctctactatc agagggcgga tgcacgcgcg
    50281 cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg atcgtttatt aaacgatctc
    50341 cgtggcctca ttggttcgga gcaggtggaa ctggagtttg actaatacag gaatactatg
    50401 agtctgaatt tccttgattt tgaacagccg attgcagagc tggaagcgaa aatcgattct
    50461 ctgactgcgg ttagccgtca ggatgagaaa ctggatatta acatcgatga agaagtgcat
    50521 cgtctgcgtg aaaaaagtgt agaactgacg cgcaaaatct tcgccgatct cggtgcatgg
    50581 cagattgcgc aactggcacg ccatccacag cgtccttata ccctggatta cgttcgcctg
    50641 gcatttgatg aatttgacga actggctggc gaccgcgcgt atgcagacga taaagctatc
    50701 gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga tcattggtca tcaaaaaggt
    50761 cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc cagcaccaga aggttaccgc
    50821 aaagctctgc gcctgatgca aatggctgaa cgctttaaga tgccaatcat cacctttatc
    50881 gacaccccgg gggcttatcc gggcgtgggc gcagaagaac gcggtcagtc tgaagctatt
    50941 gcacgcaacc tgcgtgaaat gtctcgcctc ggtgtaccgg tagtttgtac ggttatcggt
    51001 gaaggtggtt ctggcggcgc gctggcgatt ggcgtgggcg ataaagtgaa tatgctgcaa
    51061 tacagcacct attccgttat ctcgccggaa ggttgtgcgt ccattctgtg gaagagcgcc
    51121 gacaaagcac cgctggcagc tgaagcgatg ggtatcatcg ctccgcgtct gaaagaacta
    51181 aaactgatcg actccatcat cccggaaccg ctgggtggtg ctcaccgtaa cccggaagcg
    51241 atggcggcat cgttgaaagc gcaactgctg gcggatctgg ccgatctcga cgtgttaagc
    51301 actgaagatt taaaaaatcg tcgttatcag cgcctgatga gctacggtta cgcgtaattc
    51361 gcaaaagttc tgaaaaaggg tcacttcggt ggcccttttt tatcgccacg gtttgagcag
    51421 gctatgatta aggaaggatt ttccaggagg aacacatgaa catcattgcc attatgggac
    51481 cgcatggcgt cttttataaa gatgagccca ttaaagaact ggagtcggcg ctggtggcgc
    51541 aaggctttca gattatctgg ccacaaaaca gcgttgattt gctgaaattt atcgaacata
    51601 accctcgaat ttgcggcgtg atttttgact gggatgagta cagtctcgat ttatgtagcg
    51661 atatcaatca gcttaatgaa tatctcccgc tttatgcctt catcaacacc cactcgacga
    51721 tggatgtcag cgtgcaggat atgcggatgg cgctctggtt ttttgaatat gcgctggggc
    51781 aggcggaaga tatcgccatt cgtatgcgtc agtacaccga cgaatatctt gataacatta
    51841 ccccaccgtt cacgaaagcc ttgtttacct acgtcaaaga gcggaagtac accttttgta
    51901 cgccgggtca tatgggcggc accgcatatc aaaaaagccc ggttggctgt ctgttttatg
    51961 actttttcgg cgggaatact cttaaggctg atgtatctat ttcggtcacc gagcttggtt
    52021 cgttgctcga ccacaccggg ccacacctgg aagcggaaga gtacatcgcg cggactttcg
    52081 gcgcggaaca gagttatatc gttaccaacg gaacatcgac gtcgaacaaa attgtgggta
    52141 tgtacgccgc gccatccggc agtacgctgt tgatcgaccg caactgccat aaatcactgg
    52201 cgcatctgtt gatgatgaac gatgtagtgc cagtctggct gaaaccgacg cgtaatgcgt
    52261 tggggattct gggtgggatc ccgcgccgtg aatttactcg tgacagcatc gaagagaaag
    52321 tcgctgccac cacgcaggca caatggccgg ttcatgcggt gattaccaac tcaacctatg
    52381 atggcttgct ctacaacacc gactggatca aacagacgct ggatgtcccg tcgatccact
    52441 tcgattccgc ctgggtgccg tacacccatt ttcatccgat ctaccagggg aaaagtggta
    52501 tgagcggcga gcgtgttgcg ggaaaagtga tcttcgaaac gcaatcgacc cacaaaatgc
    52561 tggcggcgtt atcgcaggct tcgctgatcc acattaaagg cgagtatgac gaagaggcct
    52621 ttaacgaagc ctttatgatg cataccacca cctcgcccag ttatcccatt gttgcttcgg
    52681 ttgagacggc agcagcgatg ctgcgtggta atccgggtaa acggctgatt aaccgttcgg
    52741 tggagcgggc gctgcatttt cgcaaagagg tccagcggtt gcgggaagag tctgacggtt
    52801 ggtttttcga tatctggcaa ccgccgcagg tggatgaagc cgaatgctgg cccgttgcgc
    52861 ctggcgaaca gtggcacggc tttaacgatg cggatgccga tcatatgttt ctcgatccgg
    52921 ttaaagtcac tattttgaca ccggggatgg acgaacaggg caatatgagt gaggagggga
    52981 tcccggcggc gctggtggca aaattcctcg acgaacgtgg gatcgtagta gagaaaaccg
    53041 gcccttataa cctgctgttt ctctttagta ttggcatcga taaaaccaaa gcaatgggat
    53101 tattgcgtgg gttgacggaa tttaagcgct cttacgatct caacctgcgg atcaaaaata
    53161 tgctgcccga tctctatgct gaagatcccg atttctaccg caatatgcgt attcaggatc
    53221 tggcgcaagg gatccataag ctgattcgta aacacgatct tcccggtttg atgttacggg
    53281 cattcgatac tttgccggag atgatcatga cgccacatca ggcatggcag agacagatta
    53341 aaggtgaagt agaaaccatt gcgctggaac aactggtcgg tagagtatcg gcaaatatga
    53401 tcctgcctta tccaccgggc gtaccgctgt tgatgcctgg agaaatgctg accaaagaga
    53461 gccgcacagt actcgatttt ctactgatgc tttgttccgt agggcaacat taccccggtt
    53521 ttgaaacgga tattcacggt gcgaaacagg acgaagacgg cgtttaccgc gtacgagtcc
    53581 taaaaatggc gggataactt gccagagcgg cttccgggcg agtaacgtgc tgttaacaaa
    53641 taaaggagac gttatgctgg gtttaaaaca ggttcaccat attgcgatta ttgcgacgga
    53701 ttatgcggtg agcaaagctt tctactgcga tattcttggt ttcacgctgc aaagcgaagt
    53761 ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg cttaatgggc aatatgtgat
    53821 tgagcttttc tcatttccgt tcccgccgga gcgccccagc cgaccggaag cttgcggcct
    53881 gcgtcatctg gcttttagcg ttgatgacat cgatgcggca gtggcgcacc ttgaaagcca
    53941 taacgtgaag tgtgaagcca tccgtgtcga tccatacacg caaaaacgct tcaccttctt
    54001 taacgatccg gacgggctgc cgttggaact gtatgagcag taaggcttgt catcgcggca
    54061 tttgcccggt aacgtgccgg gcattgctac tgtaaaatcg caccatcatg acactcacgc
    54121 tcaatagaca acttctcacc tcacgccaga ttctggtggc ctttagcggc gggcttgact
    54181 ccaccgttct gctgcatcag ttggtgcagt ggcggacgga aaatccgggc gtcactctac
    54241 gcgctatcca tgtgcatcac ggtttaagtg ccaatgccga tgcctgggtt aggcattgcg
    54301 agaacatctg ccaacagtgg caggtgccgc tggtggtcga gcgcgtgcaa cttgcgcaag
    54361 aaggactggg cattgaggcc caggcgcggc aggcgcgtta tcaggcattt gcccgcacct
    54421 tgttgcccgg tgaagtgctg gtcaccgcgc aacatctcga cgatcaatgt gaaacctttc
    54481 tgctggcgct aaaacgtggt agcggccctg ccgggctttc ggctatggcg gaagtttcgg
    54541 agtttgccgg aacacggctt atccgcccgt tgctcgcccg cacgcggggg gaactggcgc
    54601 agtgggcatt agcgcatggt ttacgctgga ttgaagacga aagtaatcag gacgactcat
    54661 acgatcgtaa ctttctgcgc ctgcgtgtag tgccgttatt gcagcagcgt tggccgcatt
    54721 ttgccgaagc aacggctcgc agcgccgcac tttgtgctga acaagagagc ctgctggatg
    54781 aattgctggc agatgattta gcgcactgcc aaacgccgca ggggacgctg cagattgcgc
    54841 caatgctggc gatgagtgat gcccgtcgcg cggcaattat ccgccgctgg ctggcagggc
    54901 agaatgcacc gatgccttcc cgcgacgcgc tggtgcggat ctggcaggaa gtggcgctgg
    54961 cgcgggaaga tgcctccccc tgtttacgct ttggcgcgtt tgaaatacgc cgctatcaat
    55021 cgcaactgtg gtggattaaa tccgttgccg ggcaaagcga aaccattgtg ccgtggcaga
    55081 cgtggctaca accgctggaa ttaccggcgg ggctgggaag tgtacagctt actgcgggag
    55141 gcgatattcg ccctccgcgt gcagacgaag cggtcagcgt gcgtttcaaa gcgccaggat
    55201 tgctgcatat tgtcggtcgc aacggcggac gtaagctgaa gaaaatctgg caggagctgg
    55261 gcgtgccgcc gtggctacgt gacaccacgc cactgctgtt ttatggcgaa acgctgattg
    55321 cggcggcagg ggtatttgtg acgcaagaag gtgtggctga aggtgagaag ggcgtaagtt
    55381 ttgtctggca gaaaacgctt agttaagtga aagccggata agacgcatca aacgtcgcat
    55441 ccggcaaaag tcaatcagga ttcgcttacc accaccgtac cgatttccgg gtggctaaag
    55501 ctggtaattt tatccagacg tagctcacgg gtttcgccag cggcctcgac gaccagatac
    55561 tccacatttt tgcgggagac taaatcgctg gcttttgcct gcaatttttc gccatctttc
    55621 agctcaagtg tcagcattaa atgatgctgg caggcgagct caagattatc gtaatcatca
    55681 caattgattg gttgatacgt atcattcatt gacataatcg ctcaccagta agtttgccgc
    55741 agcgtatgct gctttttccc tgacagcctc agaaagggcg tcgtcggcag ccatttcatt
    55801 cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat cctaagtctc cgctggcgat
    55861 ttccgcgtac cgcttgcgta ttaactcaca atatttttcc acatgccctc ctgtcagcac
    55921 tctgacttaa ccgtggatgc aagtctaagc ctacgaagat aaactctgtt tcgcaaggtg
    55981 actataccac actcatttct gcaatatcag cgccgcaact gcacgtattc cgttacaatg
    56041 gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag cgacaattta taaagcgacg
    56101 gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg cctctctgac gctggcgcgc
    56161 cacccttcag aaacccagga gcgtatgatg ctgcgcttgc tggcgtggtt gaaatatgct
    56221 gatgaacgtc tgcaatttac ccgtggtttg tgtgccgatg atgagccgga agcgtggctg
    56281 cgtaacgatc acctgggcat tgatttgtgg attgagctgg ggctgccgga tgagcggcgg
    56341 attaagaaag cctgcaccca ggccgcagaa gtggcgctgt ttacctataa tagtcgggcg
    56401 gcgcaaatct ggtggcagca aaatcagagc aaatgtgtgc agtttgccaa tctttccgtc
    56461 tggtatctgg acgatgaaca actggcgaaa gtaagcgcct ttgccgatcg taccatgacg
    56521 ctgcaggcaa cgattcagga tggcgtgatc tggttatcgg atgataagaa taatctggaa
    56581 gtgaacttaa ccgcctggca acaaccttca tgattgtgat ttcccgacat gttgctattc
    56641 ccgatggtga gcttgagatc accgccattc gtgcgcaggg cgcgggcggg cagcatgtta
    56701 ataagacctc aacggctatt catctgcgtt ttgacattcg ggcgtccagc ctgccagagt
    56761 attacaaaga gcgtctgctc gccgccagcc atcatttgat cagcagtgat ggcgtgattg
    56821 tcattaaggc acaggaatac cgcagtcagg aactgaaccg cgaagcagct ctggcccggc
    56881 tggtggctgt gattaaagaa ttaacaacag aacaaaaagc ccgacgaccc acgcggccca
    56941 cccgtgcatc gaaagagcgc aggctggcat cgaaagcaca aaaatcaagc gtgaaggcga
    57001 tgcgcggcaa agtgcgcagc ggtcgggaat aaaaagaagg aatggatggt gaaaaaagcg
    57061 atagtgacag cgatggctgt aatcagcctc tttactctga tgggttgtaa taatcgggcc
    57121 gaagtcgata cgctttctcc ggcgcaggtt gccgaactga aaccgatgcc gcaaagttgg
    57181 cgcggcgtgc tgccgtgtgc cgattgcgaa ggaatcgaaa cctctctgtt cctcgaaaaa
    57241 gacggaacat gggtgatgaa tgagcgttat ctcggtgctc gtgaagaacc ttcctccttc
    57301 gcttcctacg gtacatgggc gcgaaccgct gacaagctgg tattaaccga cagcaaaggt
    57361 gaaaagtcat attatcgggc gaaaggcgat gcgctggaga tgctcgatcg tgaaggcaat
    57421 ccgattgaat cgcagttcaa ctatacgctg gaaccggcac aatccagttt acccatgacg
    57481 ccgatgaccc tgcggggcat gtatttttat atggctgatg cggcgacctt cactgattgc
    57541 gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc tggagcgtgg ctacctggct
    57601 gcgcgcggcc acagtgaaaa accggtgtta ctgtcagtag aaggtcactt tacgcttgag
    57661 gctaatccgg ataccggtgc gccaaccaaa gtattagcac ccgatacggc agggaaattt
    57721 tacccaaacc aggattgcag tagtttgggg cagtaacccg tcttgagaca gaaacaaacg
    57781 caaaacaggc cagaaggata tatttcaaca ttttgaattt gcacgttttt tgtaggccgg
    57841 ataaggcgtt tacgccgcat ccggcaatgg tgctcaacgc ctgatgcgac gctggcgcgt
    57901 cttatcatgc ctacaacccc cctcatacct taaggcggat aaggcattta ctttatcacc
    57961 gggtcaaacc aacaaagcga cccgccttga tataaatccc tggcttcagg tgcccaacat
    58021 actgtaactg cgtttctggc ttaaacgcag agacatcacc aatacgcata tgcatcagat
    58081 cggcagggct aatccagccc gattgcgcca gcgtaagcgg atgcccggct ttggcaaatg
    58141 catcagtgac aaattccgaa caaaaccacg actttttgtc tccttcgccc acactgctta
    58201 actgcgcttt cgccaggccg ctgacgcact gttggcgaaa atctgcggaa aaaggattca
    58261 gtgagcatat ctggcgggtc accataaatg gaatgaactc gacaatgccg cgatagttat
    58321 acccgctatc tttaattttg ttggcgaacg cggtgatttc tgtggcttgt tgcggggtaa
    58381 gatccgggac tcgtaagacg aaaagcttat cgctatgctt catcgccttt ttaagggaaa
    58441 caatctggac gccagcgcct gtcgcttctg caacgttatt ttcacccaga tagattgcaa
    58501 cgtgactcac agaggaagtg ctgaagacgc ggattccaaa tgaggttacc ccaaggcttg
    58561 aggagaacag caaatcgccg ggtttgagat caggtaccgt tatttctttt attgcttgtt
    58621 cggtgaaaga gctttgatgc tggaatttga cagcccatgt ttttgcctcg acatctaccg
    58681 ctgtggcaga ggagtctggc tggctgatat caacggtaca ggccgaaagt aaaagaaagc
    58741 aggggagaag caggcggcag tacgcctttg gtttatccat tttatacaat ccatgtaaaa
    58801 aagggccctg aaattcagga ccctttctgg catcagcctt taatctgttt caccagatat
    58861 tcgacgatgt caccagtctt aattaactgt ttctcgccgt tgcgacgata tttatattcg
    58921 atatcgtcgt tgtcgaggtt acggtcgccc agcacaatag tgtgcggaat accgatcagt
    58981 tccatatcag caaacatcac gcccggacgc tctttgcggt catccagcag cacttcgata
    59041 ccttgcgcgc gcagttcgct gtacagtttc tcagcaagct cttgtacgcg gaaggatttg
    59101 tgcatgttca tcggcagaat cgccacctgg aacggcgcga tagcgtcagg ccatacgatg
    59161 ccgcgttcgt cgtagttctg ctcaatcgcc gcagctacca cacgcgttac cccgataccg
    59221 tagcaaccca tcgtcaggat ttggttacgg ccatcttcac cctgtacgga ggctttcagt
    59281 gcttcggagt acttggtacc cagctggaag atgtgaccaa cttcgatacc acgtttgatc
    59341 agcagcgtac cctggccatc cgggcttgga tcgccagcca ccacgttacg gatatccgca
    59401 acttccgggg tagcgacatc gcgatcccag ttgatgccga agtagtgttt accatcgatg
    59461 ttagcaccag cagcgaaatc actcatcgcc gcaacggtac ggtcaatcac caccggaatc
    59521 ggcatgttta ccggacccag tgaacccgga ccggcgttaa ccacggcacg aatttcttct
    59581 tcggtcgcga aagtcagcgg gcttgcaacc tgcggcagtt tttctgcttt aacttcgttc
    59641 agttcgtggt caccgcgcac cagcagtgca accagcggga agctactgcc ttcaaccgct
    59701 ttaaccagca gagtcttaac cgttttctca atcggcagat tgaactgttc aaccagttcc
    59761 gcgatggttt tcgcgttcgg cgtatcaacc agcgtcattt cctgggtagc agcagcgcgc
    59821 ggttctttcg gcgcgatagc ttctgccagt tcaatgttcg ctgcatagtc agaggtgtcg
    59881 gagaagacca catcgtcttc accgctctgc gccagcacct ggaattcgtg agaggcgctg
    59941 ccgccgatag aaccggtgtc ggcctgtacg gcgcggaaat ccagccccat gcggctgaag
    60001 attttgctgt aggccgcata cattgcatcg taggtttcct gcagggattc ctgagaagta
    60061 tggaaagagt aagcatcttt catcaggaat tcgcgggaac gcatgacgcc gaaacgcgga
    60121 cgcacttcat cgcggaactt ggtctggatc tgatagaagt tcagcggcag ctgtttgtaa
    60181 gagctaagct cgttacgaat caggtcagtg ataacttctt catgagttgg gccgagtacg
    60241 aacggacgct cgccacggtc aacaaaacgc agcagttccg gaccgtactg ttcccaacga
    60301 ccactctctt gccacaaatc ggctggctga accaccggca tcgacacctc gatcgcaccg
    60361 gcgttgttca tctcttcacg cacgatgttt tcgacttttt tcagaacgcg cacgccggtc
    60421 ggcagccagg tatataaccc ggaggccagc ttgcggatca tcccggcgcg cagcatcagc
    60481 tgatggctga tcacctcggc gtcggcaggt gtctccttga gagtggagag caggtattgg
    60541 ctagtacgca tgttgttacg gttccagttg gaaggtagaa caggctcaag gcgagcctgg
    60601 gacaaaaaaa gtgatttagt ttaccagtgc aaaagaaatg tcaaaagaga agggcgtgaa
    60661 tttaacgcgg ttccagcgca aagacttcaa aacctgcgtc ggtgacgcgc cagcgaacgt
    60721 taaaatcatg tagccagacg gcataggttt tgcccgtttc ctcaccttta cgataggccg
    60781 ggcgcgggtc ctgcgccagt acttcgcgga taaacagcgt taactgcgga taacgcttct
    60841 ccagcgtcaa aagctgcttt tcgacctctg cggtaaaact caccgtcatc tctgccgctg
    60901 gcgcactttg cgcatagctg gcactggcat cgggaagcga ttcggcaaag gggagatacg
    60961 gtttgatatc caccaccggc gtaccatcga ccagatccag actgccgagc ttcagaatca
    61021 cgctgtcttt atggcaaaca acctctttca gctctaccag cgacatacca attgggttag
    61081 ggcgaaaagt ggaacgcgta gcgaaaacac ccattctggc attaccgcca agacgaggcg
    61141 gacgcacagt cggacgccag ccgccttcca tcgtttgatg aaagacgaaa agtatccata
    61201 aatggctgaa tgcttccagg ccgcgaacgg cgtcggcctg gttgtaggga gcaatgagat
    61261 gcagttcgcc gttggcgctt tttaccagac ctggctggcg cggaacggcg aatttttctt
    61321 tatagggcga gcgaataacg cctatttgct cgaactggaa actgctcatt tcgccgtaat
    61381 gttaagcgca gaaccgatac atacagcctg acgatagcag cctggcgtac cgctggtgac
    61441 ttcgcagcta tgcagtaata ccgcattggc tttcattttc gaggcgttga tttgcatccg
    61501 cttacgcgcg gttggaatgc tcggcggaga gtcctgatta gaggcctggc aagagtcgcc
    61561 actgacttca ccgagatcgc ggaacggttt gccgactaat tcttctgcat tggtataaat
    61621 tcggaccggc gtggcgcgcg gcgctttcgg ttttgcaggc tccgctttcg gctggggtgc
    61681 agtgctttga acgggttcga caggggatct gcttaacatg gaacagccgc ttagcatgag
    61741 tgctactaaa cagatcggta aagcacgcat agtatttcct caatgtatga tcaaaacgtc
    61801 aatattgaat caggagcttg taaaaatgac aagacgggca agcgcccgtc ctgaatgata
    61861 ttacaaattg tggaaacagc ctaaaaatta ccagccttta acagcgccgc cgttaaacac
    61921 tttgtttgct gcttcgtaaa cttcgtcaga ctgataagcc tggacgaatt tcttcacgtt
    61981 ctcggcgtct ttgttatctt cacgcgttac gatcaggttt acgtacgggg agtctttatc
    62041 ttcgacaaag ataccgtctt tcgctggagt caggccaatc tggctggcat aggtggtatt
    62101 gataactgcc agagcgattt gcgcatcgtc cagagaacgc ggcagttgcg gtgcttccag
    62161 ttcaacaatt ttcagatttt ttgggttctc aacaacatca agaacggtcg gcagcaggcc
    62221 aacgccatct ttcagtttga tcaagcccac tttttgcagc agcagcagtg aacgaccaag
    62281 gttagttggg tcgtttggca cggcaacctg cgaaccatcc tgcagttcat ccagtgattt
    62341 gattttcttg gagtaaccag caatcggata aacaaaagtg ttgcctactg cgaccagttt
    62401 gtagccacga tctttcagtt gctgatcaag gtacggttta tgctggaagg cgttggcgtc
    62461 gatatcgcct ttgctcaatg cttcgtttgg cagaacatag tcgttgaagg ttaccagctc
    62521 aacgtccagg ccatatttgt ctttcgcaac tttctgcgcg acttctgcaa cctgctgttc
    62581 ggcaccaaca atcacgccga ctttaatgtg gtttggatct ttttcatcct gaccgcagcc
    62641 taccagtgcc agtgatccaa tcagggctcc cactgccgca aaggttttga atttgaacgc
    62701 cataccttat tccttcttca attatttatg ttgtgttgaa cgttacttgc gagtgacagc
    62761 ccggacgatg cggtcgcctg cgaactgaat taaataaacc agaatgacca gcaataccag
    62821 taccgtattc atcaccgtcg cgttgtagcc gatgtagcca tactgatagc caatctgacc
    62881 taaaccaccg gcaccgactg caccacccat cgcggaataa ccgaccaggg taatcagggt
    62941 gatagttgcc gcattcacca gacccggcaa cgcttccggt aacagcactt tacggacgat
    63001 ctgcattggc gtggcaccca ttgcgcggga agcttcaatt aacccggttg ggatctccag
    63061 cagagcgttc tcgaccatac gggcaataaa cggtgctgca ccaacggtta acggaacaat
    63121 tgctgcctgc aaaccaatcg atgtaccgac aataacgcgg gtaaacggaa tcatccatac
    63181 cagcaagata atgaacggga tggaacggaa aatgttcaca atcgcagaaa cggtacgata
    63241 cagcttcgcg ttagcaataa tttgccccgg acgcgtgaca taaagcagga cgccaaccgg
    63301 cagaccaatc acaaacccaa aaaatccgga tacgaaggtc attgccagcg tttcccatac
    63361 gccacgaacc agcagccaca tcatcggctc agacataacc cagtacctct acttttacat
    63421 ggtgttcctg cagccaggca atggcggctt gcgtatcttg ttgtgtgccg tgcatttcag
    63481 tcagcatgat gccgaattta acgccaccag cgtagtccat ctgcgcgcta ataatgttgt
    63541 tgttgacgtt gaaacgacgc gcggtttcag aaagcagtgg ggcatcgacc gattgaccgg
    63601 taaactccag acgcagcatc ggcacgcagt cagtgaatgg ctccgcttgc agacgttcct
    63661 ggtaatcttc cgggatatcc agatgcaggg ttgactgaat aaacttctgc gccagcggcg
    63721 ttttcggatg tgagaacact tcacttaccg tgtcctgctc gattagttcg ccattgctga
    63781 tgaccgcaac gcaatcacaa atacgcttca caacgtccat ttcgtgggtg atcaacagaa
    63841 tcgtcaaacc cagacggcgg ttgatgtctt tcagcagttc gagaatagaa cgtgtcgttg
    63901 ccgggtccag cgcgctggtg gcttcatcac acagcaatac tttgggattg ctggctaatg
    63961 cacgggcaat cgccacacgt tgtttctgcc caccggaaag attcgacggg tagctatcat
    64021 gcttatcgcc aagaccaacc aatgacagca attccgtcac gcgacgtttg atctcgtctt
    64081 tcggtgtatt gtccagctcc agcggcagag ccacgttgcc aaaaacagta cgcgaagaga
    64141 gcaggttaaa atgctggaaa atcataccaa tctggcgacg agctttggtc aactcggatt
    64201 ctgacagcgt ggtcagttcc tggccatcga ccagcacgct accctcggtt gggcgctcca
    64261 gcaggtttac acaacgtata agcgtactct tacccgcgcc tgaggcaccg ataacgccat
    64321 aaatttgtcc agctggcaca tgcaggctga cgttgttcaa cgcctggatg atgcgggtgc
    64381 cctggtggaa cactttggtg atattcgaaa gttttatcat tgattattta ttatcgtcat
    64441 taagttagtc gtggcatctc gaatgcctga aacgggcaac gccgtcaatg aaatggatgt
    64501 taaggcatcc agacgtctaa atcaatcagg tttatgcgaa gagcactttc ttgcaggtcg
    64561 aaacatgcga tactagcgtc acatgcctta ttaaggagct ataaaaggtg gcgaagagcg
    64621 tacccgcaat ttttcttgac cgtgatggca ccattaatgt cgatcacggc tatgtccatg
    64681 agatcgacaa ctttgaattt atcaacggtg ttattgacgc catgcgcgag ctaaaaaaaa
    64741 tgggctttgc gctggtggta gtaaccaacc agtctggcat tgctcgcggt aaatttaccg
    64801 aagcacagtt tgaaacgctg accgagtgga tggactggtc gctggcggac cgagatgtcg
    64861 atctggatgg tatctattat tgcccgcatc atccgcaggg tagtgttgaa gagtttcgcc
    64921 aggtctgcga ttgccgcaaa ccacatccgg ggatgctttt gtcagcacgc gattatttgc
    64981 atattgatat ggccgcttct tatatggtgg gcgataaatt agaagatatg caggcagcgg
    65041 ttgcggcgaa cgtgggaaca aaagtgctgg tgcgtacggg taaacctatt acacctgaag
    65101 cagaaaacgc ggcagattgg gtgttaaata gcctggcaga cctgccgcaa gcgataaaaa
    65161 agcagcaaaa accggcgcaa tgattaaaag atgagcggtt gaaataaaaa tgcatttttc
    65221 cgcttgtctt cctgagccga ctccctataa tgcgcctcca tcgacacggc ggatgtgaat
    65281 cacttcacac aaacagccgg ttcggttgaa ga
//
LOCUS       DAZFKF010000020        59092 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFKF010000020 DAZFKF010000000
VERSION     DAZFKF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.020"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(45..647)
                     /gene="fau"
                     /locus_tag="ACHKA1_001536"
     CDS             complement(45..647)
                     /gene="fau"
                     /locus_tag="ACHKA1_001536"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO5679212.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRTLTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(668..851)
                     /gene="ssrS"
                     /locus_tag="ACHKA1_001537"
     ncRNA           complement(668..851)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKA1_001537"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(893..1222)
                     /gene="zapA"
                     /locus_tag="ACHKA1_001538"
     CDS             complement(893..1222)
                     /gene="zapA"
                     /locus_tag="ACHKA1_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO5679213.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            1390..1968
                     /gene="ygfB"
                     /locus_tag="ACHKA1_001539"
     CDS             1390..1968
                     /gene="ygfB"
                     /locus_tag="ACHKA1_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO5679214.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            1994..3319
                     /gene="pepP"
                     /locus_tag="ACHKA1_001540"
     CDS             1994..3319
                     /gene="pepP"
                     /locus_tag="ACHKA1_001540"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO5679215.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            3316..4494
                     /gene="ubiH"
                     /locus_tag="ACHKA1_001541"
                     /gene_synonym="visB"
     CDS             3316..4494
                     /gene="ubiH"
                     /locus_tag="ACHKA1_001541"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO5679216.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLECGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            4517..5719
                     /gene="ubiI"
                     /locus_tag="ACHKA1_001542"
                     /gene_synonym="visC"
     CDS             4517..5719
                     /gene="ubiI"
                     /locus_tag="ACHKA1_001542"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO5679217.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(5793..5876)
                     /locus_tag="ACHKA1_001543"
     CDS             complement(5793..5876)
                     /locus_tag="ACHKA1_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679218.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            6167..7261
                     /gene="gcvT"
                     /locus_tag="ACHKA1_001544"
     CDS             6167..7261
                     /gene="gcvT"
                     /locus_tag="ACHKA1_001544"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO5679219.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVEKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            7285..7674
                     /gene="gcvH"
                     /locus_tag="ACHKA1_001545"
     CDS             7285..7674
                     /gene="gcvH"
                     /locus_tag="ACHKA1_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO5679220.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDVLSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            7792..10665
                     /gene="gcvP"
                     /locus_tag="ACHKA1_001546"
     CDS             7792..10665
                     /gene="gcvP"
                     /locus_tag="ACHKA1_001546"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO5679221.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLADLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAGWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(10831..12264)
                     /gene="bglA"
                     /locus_tag="ACHKA1_001547"
     CDS             complement(10831..12264)
                     /gene="bglA"
                     /locus_tag="ACHKA1_001547"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO5679222.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDILREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            12309..12620
                     /gene="yqfB"
                     /locus_tag="ACHKA1_001548"
     CDS             12309..12620
                     /gene="yqfB"
                     /locus_tag="ACHKA1_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO5679223.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMPLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            12784..13443
                     /gene="yqfA"
                     /locus_tag="ACHKA1_001549"
     CDS             12784..13443
                     /gene="yqfA"
                     /locus_tag="ACHKA1_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO5679224.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(13520..14500)
                     /gene="ygfZ"
                     /locus_tag="ACHKA1_001550"
     CDS             complement(13520..14500)
                     /gene="ygfZ"
                     /locus_tag="ACHKA1_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO5679225.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            14743..15009
                     /gene="sdhE"
                     /locus_tag="ACHKA1_001551"
     CDS             14743..15009
                     /gene="sdhE"
                     /locus_tag="ACHKA1_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO5679226.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            14990..15397
                     /gene="ygfX"
                     /locus_tag="ACHKA1_001552"
     CDS             14990..15397
                     /gene="ygfX"
                     /locus_tag="ACHKA1_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO5679227.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(15437..15958)
                     /gene="fldB"
                     /locus_tag="ACHKA1_001553"
     CDS             complement(15437..15958)
                     /gene="fldB"
                     /locus_tag="ACHKA1_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO5679228.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            16070..16966
                     /gene="xerD"
                     /locus_tag="ACHKA1_001554"
     CDS             16070..16966
                     /gene="xerD"
                     /locus_tag="ACHKA1_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO5679229.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            16991..17701
                     /gene="dsbC"
                     /locus_tag="ACHKA1_001555"
     CDS             16991..17701
                     /gene="dsbC"
                     /locus_tag="ACHKA1_001555"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO5679230.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            17707..19440
                     /gene="recJ"
                     /locus_tag="ACHKA1_001556"
     CDS             17707..19440
                     /gene="recJ"
                     /locus_tag="ACHKA1_001556"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO5679231.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERNIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            19531..20629
                     /gene="prfB"
                     /locus_tag="ACHKA1_001557"
     CDS             join(19531..19605,19607..20629)
                     /gene="prfB"
                     /locus_tag="ACHKA1_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO5679232.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            20639..22156
                     /gene="lysS"
                     /locus_tag="ACHKA1_001558"
     CDS             20639..22156
                     /gene="lysS"
                     /locus_tag="ACHKA1_001558"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO5679233.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFGGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(22199..22747)
                     /gene="idi"
                     /locus_tag="ACHKA1_001559"
     CDS             complement(22199..22747)
                     /gene="idi"
                     /locus_tag="ACHKA1_001559"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO5679234.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNPVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            22802..22873
                     /locus_tag="ACHKA1_001560"
     CDS             22802..22873
                     /locus_tag="ACHKA1_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679235.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(22870..22995)
                     /gene="yqfG"
                     /locus_tag="ACHKA1_001561"
     CDS             complement(22870..22995)
                     /gene="yqfG"
                     /locus_tag="ACHKA1_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO5679236.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(22997..24445)
                     /gene="uacT"
                     /locus_tag="ACHKA1_001562"
     CDS             complement(22997..24445)
                     /gene="uacT"
                     /locus_tag="ACHKA1_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO5679237.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            24881..26800
                     /gene="ygfT"
                     /locus_tag="ACHKA1_001563"
     CDS             24881..26800
                     /gene="ygfT"
                     /locus_tag="ACHKA1_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO5679238.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHI
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGCDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            26800..27288
                     /gene="ygfS"
                     /locus_tag="ACHKA1_001564"
     CDS             26800..27288
                     /gene="ygfS"
                     /locus_tag="ACHKA1_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO5679239.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDCISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLLGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     LL"
     gene            complement(27324..28691)
                     /gene="ghxQ"
                     /locus_tag="ACHKA1_001565"
                     /gene_synonym="ygfQ"
     CDS             complement(27324..28691)
                     /gene="ghxQ"
                     /locus_tag="ACHKA1_001565"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO5679240.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(28727..30043)
                     /gene="guaD"
                     /locus_tag="ACHKA1_001566"
     CDS             complement(28727..30043)
                     /gene="guaD"
                     /locus_tag="ACHKA1_001566"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO5679241.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(30061..31461)
                     /gene="xanQ"
                     /locus_tag="ACHKA1_001567"
     CDS             complement(30061..31461)
                     /gene="xanQ"
                     /locus_tag="ACHKA1_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO5679242.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(31626..34496)
                     /gene="xdhD"
                     /locus_tag="ACHKA1_001568"
     CDS             complement(31626..34496)
                     /gene="xdhD"
                     /locus_tag="ACHKA1_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO5679243.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(34493..35272)
                     /gene="ygfM"
                     /locus_tag="ACHKA1_001569"
     CDS             complement(34493..35272)
                     /gene="ygfM"
                     /locus_tag="ACHKA1_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO5679244.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(35323..36651)
                     /gene="ssnA"
                     /locus_tag="ACHKA1_001570"
     CDS             complement(35323..36651)
                     /gene="ssnA"
                     /locus_tag="ACHKA1_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO5679245.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SFKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLKAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(36654..39752)
                     /gene="ygfK"
                     /locus_tag="ACHKA1_001571"
     CDS             complement(36654..39752)
                     /gene="ygfK"
                     /locus_tag="ACHKA1_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO5679246.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSKGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(40074..40652)
                     /gene="mocA"
                     /locus_tag="ACHKA1_001572"
     CDS             complement(40074..40652)
                     /gene="mocA"
                     /locus_tag="ACHKA1_001572"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO5679247.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAFQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            40755..41525
                     /gene="yqeC"
                     /locus_tag="ACHKA1_001573"
     CDS             40755..41525
                     /gene="yqeC"
                     /locus_tag="ACHKA1_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO5679248.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQT
                     RGRRVLITTTTHMFMPKSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            41573..43198
                     /gene="yqeB"
                     /locus_tag="ACHKA1_001574"
     CDS             41573..43198
                     /gene="yqeB"
                     /locus_tag="ACHKA1_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO5679249.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLTTSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(43419..44351)
                     /gene="arcC"
                     /locus_tag="ACHKA1_001575"
     CDS             complement(43419..44351)
                     /gene="arcC"
                     /locus_tag="ACHKA1_001575"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO5679250.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(44399..45784)
                     /gene="hyuA"
                     /locus_tag="ACHKA1_001576"
     CDS             complement(44399..45784)
                     /gene="hyuA"
                     /locus_tag="ACHKA1_001576"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO5679251.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(45837..47048)
                     /gene="ygeY"
                     /locus_tag="ACHKA1_001577"
     CDS             complement(45837..47048)
                     /gene="ygeY"
                     /locus_tag="ACHKA1_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO5679252.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(47106..48302)
                     /gene="dpaL"
                     /locus_tag="ACHKA1_001578"
     CDS             complement(47106..48302)
                     /gene="dpaL"
                     /locus_tag="ACHKA1_001578"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO5679253.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVFSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(48360..49547)
                     /gene="ygeW"
                     /locus_tag="ACHKA1_001579"
     CDS             complement(48360..49547)
                     /gene="ygeW"
                     /locus_tag="ACHKA1_001579"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO5679254.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            50026..51804
                     /gene="ygeV"
                     /locus_tag="ACHKA1_001580"
     CDS             50026..51804
                     /gene="ygeV"
                     /locus_tag="ACHKA1_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO5679255.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGIVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(51844..52323)
                     /gene="xdhC"
                     /locus_tag="ACHKA1_001581"
     CDS             complement(51844..52323)
                     /gene="xdhC"
                     /locus_tag="ACHKA1_001581"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO5679256.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(52320..53198)
                     /gene="xdhB"
                     /locus_tag="ACHKA1_001582"
     CDS             complement(52320..53198)
                     /gene="xdhB"
                     /locus_tag="ACHKA1_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO5679257.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDSITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(53209..55506)
                     /gene="xdhA"
                     /locus_tag="ACHKA1_001583"
     CDS             complement(53209..55506)
                     /gene="xdhA"
                     /locus_tag="ACHKA1_001583"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO5679258.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVSRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            55922..56677
                     /gene="actS"
                     /locus_tag="ACHKA1_001584"
     CDS             55922..56677
                     /gene="actS"
                     /locus_tag="ACHKA1_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO5679259.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            56756..56829
                     /locus_tag="ACHKA1_001585"
     tRNA            56756..56829
                     /locus_tag="ACHKA1_001585"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56788..56790,aa:Gly,seq:ccc)
     gene            57063..58673
                     /locus_tag="ACHKA1_001586"
     CDS             57063..58673
                     /locus_tag="ACHKA1_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFO5679260.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDIISNPKRLDGIPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAINTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKVNELDETLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLSKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            58740..58919
                     /locus_tag="ACHKA1_001587"
     CDS             58740..58919
                     /locus_tag="ACHKA1_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HFO5679261.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAIITGLTWPL
                     SLIPSIISLMIRKSD"
BASE COUNT        14516 a        14650 c        15445 g        14481 t
ORIGIN      
        1 aaagaatcaa cgatgtcaat cagggcgatg caggtgtgtc gccctcacca ctcccagact
       61 ttcgacggtg taaccaccgc aggaagaggg atatcccact cttcaacggg gagtttttcc
      121 accaactgac aatcatgcgc ataacccacc ggttgcattt tatagtgctg ccagttttgt
      181 aaggtccgat cataaaaacc accgcccatt cccaggcgct gaccgtactc atcaaaggcg
      241 accagcggtg tgatcaacac gtctaatcgg gaaaggggta gcacgtcacg cacatccagt
      301 tttggctcat ggatcttcaa cctgttcatc accagttcac tttgcggatg gtaattcagg
      361 aacagcaaat taccggcact aaagggatgc aaaactggaa gatatacgcg cttaccggcg
      421 cgccagagtt gttctataag cggctgggtg tcgagttcgc catcaaaaga gaggaataca
      481 gcgaccgtat gtgccatcac caccggggga taagtcatca tccgggtagc ggcttgttga
      541 cccatttcct gctgttgttc cggcgttaac gtacgacgac gttgccgaat cattttgcgg
      601 atttcttgtc gggataatgt cagtgggagt tctggtagtt gcgtcatggc tggtgccaga
      661 taagaaggga atctccgaga tgccgccgca ggctgtaacc cttgaaccct tggttcaagg
      721 tgaatgtgtc gtcgcagttt taaggcttct cggacggacc gagcatgctc accaaccgcg
      781 gagcgccaca ttcttgtggt atgaaatatc ggctcagggg actggcccgc ttgcgaacat
      841 ctcagagaaa ttttgtcttc acggttactc taccacagta aaccgaaaag tgtcattcaa
      901 agttttggtt agttttttcg gtgatgcgac cttgttcaag taacgcttgt tctatggtct
      961 gctgcagcat ccgaatacgc tgttccatac ttgccgcgta gtcacgagtc tttgcttttt
     1021 cttgcgctaa ctcatagctg atattcaatg cggcaatgaa gaccaactgt tcagtatttg
     1081 tgactctagt gcgttctttc agatcttgca accgttggtt cagatcgtcc gctgcctgat
     1141 tcaacgcatc cctttggtca ggcgggcagt tcacacgcag tgaacggcca aaaatttgga
     1201 tatcgacggg ttgtgcagac atgccacctt cctgctgatt gactgcgctg ccttcgtctt
     1261 tagacccatg gtctgcgaag gggcgacact atagctaccc tgatgagaag agacaagccc
     1321 ttttctggtc caccagggcc caaagtggta gcatatcatg aatattcctc cctttgacga
     1381 cgaatgctta tgtctataca gaacgaaatg cctggttaca acgaaatgaa ccagtatctg
     1441 aaccaacaag ggacgggtct gacccctgct gagatgcatg gtttaatcag tgggatgata
     1501 tgtggcggta acgatgacag ctcatggctg ccgctacttc acgacctgac gaacgaaggc
     1561 atggctttcg gtcatgagct ggcacaggca ctgcgtaaaa tgcactctgc caccagcgat
     1621 gccctgcagg atgacggctt cctttttcag ctttatctgc ctgatggcga tgatgtcagc
     1681 gttttcgatc gggctgatgc gctggccggt tgggtcaatc acttcctgct tggtcttggc
     1741 gttacgcaac cgaagctgga caaagtgacc ggcgaaaccg gtgaagccat cgacgatctg
     1801 cgtaacatcg cgcagttggg ctacgacgaa gacgaagatc aggaagagct tgaaatgtcg
     1861 cttgaagaga tcatcgaata cgttcgtgtt gccgcgctgt tatgccacga cacctttact
     1921 catccgcaac cgaccgcgcc agaagtacaa aaaccgactc tacactaaaa acaaaaaacg
     1981 taaggagagt gttatgagtg agatatcccg gcaagagttt cagcgtcgcc gtcaggccct
     2041 ggtggagcaa atgcaacccg gcagcgccgc gctgattttt gctgcaccag aagtaacacg
     2101 tagcgccgac agcgaatacc cctatcgtca gaacagtgac ttctggtact tcaccggctt
     2161 taacgaaccg gaagcggtgc tggtgctgat taaaagcgat gacactcata accacagcgt
     2221 tctgtttaac cgcgttcgcg acctgacggc ggagatctgg tttggccgtc gtttaggtca
     2281 ggatgccgcg ccagagaaac tgggcgttga ccgcgcactg gcattcagcg aaattaatca
     2341 gcaactttat caactactta atggtctgga tgtggtttac cacgcccagg gcgaatatgc
     2401 atatgctgat gaaatcgtga acagcgcgct tgagaaactg cgtaaaggtt cgcggcaaaa
     2461 tctcaccgcg ccggcaacga tgatcgactg gcgtcccatt gttcatgaaa tgcgcctgtt
     2521 caaatcgccg gaagagattg ccgtacttcg ccgcgcgggt gaaatcaccg ccctggcgca
     2581 tacccgggcg atggaaaaat gccgtccggg aatgttcgag taccacctgg aaggcgaaat
     2641 tcaccacgaa tttaaccgcc acggcgcgcg ctatccttcc tataacacca ttgtcggcag
     2701 cggtgaaaac ggctgcattc tgcattacac cgaaaacgag tgtgaactgc gtgacggaga
     2761 tctggtgttg attgatgcgg gttgtgaata caaaggttac gctggcgata ttacccgcac
     2821 cttcccggtc aacggcaaat tcacccaggc tcagcgtgaa atctacgaca ttgtgcttga
     2881 gtctctcgaa accagcctgc gcctgtatcg tccgggaact tccattcagg aagtcaccgg
     2941 tgaagtggtg cgcatcatgg ttagcggcct ggtaaaactc ggcatcctga aaggtgatgt
     3001 tgatgaactg atcgctcaga acgcccatcg tcctttcttt atgcatggcc ttagccactg
     3061 gttaggactg gatgtccatg acgttggcgt ttatggccag gatcgctcgc gcattctgga
     3121 accgggcatg gtactgaccg tagagccagg gctgtatatt gcgccggatg cagaagtgcc
     3181 agaacaatat cgcggtatcg gcattcgtat tgaagacgac attgtgatta ccgaaaccgg
     3241 taacgaaaac ctcaccgcca gcgtggtgaa aaagccggaa gaaatcgaag cgttgatggc
     3301 agctgcgaga aagcaatgag cgtaatcatc gtcggtggcg gcatggcggg cgcgacgctg
     3361 gcgctggcta tttcccggtt aagtcacggg gcgctgccgg tacatttgat tgaagcgacc
     3421 gcgccagagt cacatgctca tccgggcttt gatgggcgag cgattgcgct ggcggcgggt
     3481 acctgtcagc aactggcgcg cattggcgtc tggcaatctc tggcggattg cgcaaccgcc
     3541 atcactaccg tgcatgtcag cgatcgtggt catgccggat tcgtcaccct cgccgcagaa
     3601 gattaccaac tggcggcgct ggggcaggtt gtcgaattgc acaatgtcgg gcaacggctg
     3661 tttgcattgc tgcgcaaagc acctggcgta acgctgcatt gcccggatcg cgtggctaac
     3721 gttgcccgta ctcagagtca tgttgaagtg acgctggagt gtggcgagac gctgacgggc
     3781 cgcgtgctgg tagccgctga tggcacccat tcagcgttag ccactgtgtg cggcgttgac
     3841 tggcagcagg agccttacga acaactggct gtgattgcca acgttgcgac ttccgttgcg
     3901 catgaagggc gcgcttttga acgctttacg caacatggcc cgctggcgat gttgccgatg
     3961 tctgacggac gctgttcgct ggtctggtgt catccactgg aacggcgcga agaggtgctg
     4021 tcgtggagcg acgagaagtt ttgccgtgaa ctccagtcgg cctttggctg gcgacttggg
     4081 aaaattaccc acgctggtaa acgcagtgct tatccgctgg cgttaaccca cgccgccaga
     4141 tctattaccc atcgtaccgt gctggtgggc aatgcggcgc aaaccctgca cccgattgcc
     4201 gggcaagggt tcaacctcgg tatgcgagat gtcatgagtc ttgcggaaac cctgactcag
     4261 gcgcaggagc gcggagaaga catgggtgat tacggcgtat tgtgtcgtta tcagcagcgt
     4321 cgacagagcg atcgcgaagc aaccattggc gtcacggaca gccttgtaca tctttttgcc
     4381 aaccgttgga cgccactggt tgtcgggcgc aacatcgggc tgatgacgat ggaattattc
     4441 accccggcac gcgatgtgct ggcgcagcgc accctcggtt gggtggcgcg ttgaaggcgg
     4501 ttttaaggaa taaacaatgc aaagtgttga tgtagccatt gttggcggcg gcatggtggg
     4561 gctggcggtt gcctgcggct tacaggggag cggcttacgc gttgccgtac tggagcagcg
     4621 cgtaccggaa cctctggcgg tggatgcacc accacaactg cgcgtttcgg ctatcaatgc
     4681 cgccagcgaa aaattactca cccgtcttgg cgtctggcaa gacattctct ctcgtagggc
     4741 cagttgctat cacggtatgg aagtgtggga caaagacagt tttggtcaca tttcgtttga
     4801 cgatcaaagc atgggctata gccatcttgg gcatatcgtt gaaaactcag tgattcacta
     4861 cgcgctgtgg aacaaagcgc agcagtcgtc agatatcact ctgttggccc ccgcagaatt
     4921 acagcaggtc gcctggggag aaaacgaaac cttcctgacg ctgaaagatg gcagtatgtt
     4981 aacggcgcgt ctggtgattg gcgcggacgg cgctaattcc tggttgcgca acaaagccga
     5041 tattccgctg actttctggg attatcagca tcacgcgctg gtagcgacca tacgcacgga
     5101 agaaccgcat gatgcggtgg cgcggcaggt tttccatggc gaaggcattc tggccttttt
     5161 accgcttagc gatccgcatc tttgctcgat tgtctggtca ctgtcgccag aggaagcgca
     5221 gcggatgcag caggcaagtg aagacgaatt taatcgcgcg ttaaatatcg cttttgataa
     5281 tcgccttggc ttatgcaagg ttgagagcgc gcgtcaggtg ttcccactga cggggcgtta
     5341 tgcgcgccag tttgccgcgc atcgtctggc gctggtgggc gacgccgcgc ataccattca
     5401 cccgctggcg gggcaggggg taaatctcgg ctttatggat gctgcagagc tgattgctga
     5461 actgaaacgg ttgcatcgtc agggtaaaga catcgggcag tacatttacc tgcgccgcta
     5521 tgaacgtagc cgcaagcaca gcgcggcgct gatgctggct ggtatgcagg gttttcgcga
     5581 tctcttttcc ggcgctaatc cggcgaaaaa actgctgcgt gatattggtt tgaaactggc
     5641 cgacacgctt cctggcgtta agccgcaact tatccgccag gcaatgggat taaacgattt
     5701 gcctgaatgg ctgcgttaaa aatttctcct ctgttgttta tttgataccc atcacacttt
     5761 catctcccgg ttttttcccc gggagatttt cctcatttga aataaactaa tttcacctcc
     5821 gttttcgcat tatattttct aatgccatga ttttttgatt tagtgttttt tgacattttt
     5881 ttagctctta atattgtctt attcaaattg actttctcat cacatcatct ttgtatagaa
     5941 actggtgtgt tttttggttt tttattctgt cgcgattttt gcatttttta accataagct
     6001 aatgtgatga tcaattttac cttatggtta acagtctgtt tcggtggtaa gttcaggcaa
     6061 aagagaacga ttgcgttggg gaccgggagt ggctccgatg ctgggttttg tggtgataat
     6121 ttcaccatga aaaagttgtc agccccgctt attcaatgag gacaagatgg cacaacagac
     6181 tcctttgtac gaacaacaca cgctttgcgg cgctcgcatg gtggatttcc acggctggat
     6241 gatgccgctg cattacggtt cgcaaatcga cgaacatcat gcggtacgta ccgatgccgg
     6301 aatgtttgat gtgtcgcata tgaccatcgt cgatctccgc ggcagccgca cccgggagtt
     6361 tctgcgttat ctgctggcga acgatgtgga gaagctcacc aaaagcggca aagcccttta
     6421 ctcggggatg ttgaatgcct ctggcggtgt gatagatgat ctcatcgtct actactttac
     6481 tgaagatttc ttccgcctcg ttgttaactc cgccacccgc gaaaaagacc tctcctggat
     6541 tacccaacac gctgaacctt tcggcatcga aattaccgtt cgcgatgacc tttccatgat
     6601 tgccgtgcaa gggccgaatg cgcaggcaaa agctgccaca ctgtttaatg acgcccagcg
     6661 tcaggcggtg gaagggatga aaccgttctt tggcgtgcag gcgggcgatc tgtttattgc
     6721 caccactggt tataccggtg aagcgggcta cgaaattgcg ctgcccaatg aaaaagcggc
     6781 ggatttctgg cgcgcgctgg tggaagcggg cgttaagcca tgcggcctgg gcgcgcgtga
     6841 cacgctgcgt ctggaagcgg gtatgaatct ttatagtcag gagatggacg aaaccatctc
     6901 tcctttagcc gccaatatgg gctggactat cgcctgggaa ccggcagatc gtgacttcat
     6961 cggtcgtgaa gccctggaag tgcagcgtga gcatggtaca gaaaaactgg ttggtctggt
     7021 gatgaccgaa aaaggcgtgc tgcgtaatga actgccggta cgttttaccg atgcgcaggg
     7081 caaccagcat gaaggcatta tcaccagcgg gactttctcc ccgacgctgg gttacagcat
     7141 tgcgctggcg cgcgtgccgg aaggtattgg tgaaacagcg attgtgcaaa ttcgcaaccg
     7201 tgaaatgccg gttaaagtga cgaaacctgt ttttgtgcgt aacggcaaag ccgtcgcgtg
     7261 atttactttt ttggagattg attgatgagc aacgtaccag cagaactgaa atacagcaaa
     7321 gaacacgaat ggctgcgtaa agaagccgac ggcacttaca ccgttggcat caccgaacac
     7381 gctcaggagc tgttaggcga tatggtgttt gttgacctgc cggaagtggg cgcgacggtt
     7441 agcgcgggcg atgactgcgc ggttgccgaa tcggtaaaag cggcgtcaga catttatgca
     7501 ccagtaagcg gtgaaatcgt ggcggtaaac gacgtactga gcgattcccc ggaactggtg
     7561 aacagcgaac cgtatgcagg cggctggatt ttcaaaatca aagccagcga tgaaagcgaa
     7621 ctggaatcac tgctggatgc gaccgcatac gaagcattgt tagaagacga gtaacggctt
     7681 tattcctctt ctgcgggaga ggatcagggt gaggaaattt acgcctcacc ctcactctct
     7741 tcgtaaggag agaggttcac aattcactgc acgtttcagg aaccatcgct catgacacag
     7801 acgttaagcc agcttgaaaa tagcggcgct tttattgaac gccatatcgg accggacgcc
     7861 gcgcaacagc aagaaatgct gaatgccgtt ggcgcacaat cgttaaacgc gctgaccggc
     7921 cagattgtac cgaaagatat tcagcttgcg accccgccgc aggttggcgc accggcgacc
     7981 gaatacgctg cactggcaga actcaaggct attgccagtc gcaataaacg cttcacgtct
     8041 tacatcggca tgggttacac cgccgtgcag ttaccgccgg ttatcctgcg taacatgctg
     8101 gaaaatccgg gctggtatac cgcgtatact ccgtatcaac ctgaagtctc gcagggccgc
     8161 cttgaggcac tgctcaattt ccagcaggta acgctggatt taaccgggct ggatatggcc
     8221 tctgcttctc ttcttgacga agccaccgct gccgccgaag caatggcgat ggcgaaacgc
     8281 gtcagcaaac tgaaaaatgc caaccgcttc tttgttgctt ctgacgttca tccgcaaacg
     8341 ctggatgtgg tacgtactcg tgctgaaacc tttggttttg aagtgattgt cgatgatgcg
     8401 caaaaagtgc tcgaccatca ggacgtcttc ggcgtgctgt tacagcaggt cggtactact
     8461 ggtgaaattc acgactacac tgcgcttatc agcgaactga aatcacgcaa aattgtggtc
     8521 agcgtcgccg ccgatattat ggcgctggtg ctgttaactg cgccgggtaa acagggcgca
     8581 gatattgtct ttggttcggc gcaacgcttc ggcgtgccga tgggctacgg tggcccacac
     8641 gcggcattct ttgcggcgaa agatgaatac aaacgctcaa tgccaggccg tattatcggt
     8701 gtatcgaaag atgcagctgg caataccgcg ctgcgcatgg cgatgcagac tcgcgagcaa
     8761 catattcgtc gtgagaaagc gaactccaac atttgtactt cccaggtgct gctggcaaac
     8821 atcgccagcc tgtatgccgt ttatcacggc ccgattggcc tgaaacgtat cgctaaccgc
     8881 attcaccgtc tgaccgatat cctggcggca ggcctgcaac aaaaaggtct gaagctgcgc
     8941 catgcgcact atttcgacac tctgtgtgtg gaagtggccg acaaggcggg cgtactggcg
     9001 cgtgccgaag cggctgaaat caacctgcgt agcgatatcc tgaacgcggt tgggatcacc
     9061 cttgatgaaa ccaccacgcg cgaaaacgtg atgcagcttt tcagcgtact actgggggat
     9121 aatcacgggc tggacatcga cacgctggac aaagacgtgg ctcacgacag ccgctctatt
     9181 caacctgcga tgctgcgtga tgacgaaatc ctcacccatc cggtgtttaa tcgctaccac
     9241 agcgaaaccg aaatgatgcg ctatatgcac tcgctggagc gtaaagatct ggcgctgaat
     9301 caggcgatga tcccgctggg ttcctgcacc atgaaactga acgccgccgc cgagatgatc
     9361 cccatcacct ggccggaatt tgccgaactg caccctttct gcccgccgga acaggccgaa
     9421 ggttatcagc agatgatcgc gcagctggct gactggctgg tgaaactgac cggttacgac
     9481 gccgtctgta tgcagccgaa ctctggcgca cagggcgaat acgcgggcct gctggcgatt
     9541 cgtcattatc atgaaagccg caacgaaggg catcgcgata tctgcctgat cccggcttct
     9601 gcgcacggca ctaaccctgc ttctgcacat atggcaggaa tgcaggtggt ggttgtggca
     9661 tgtgataaaa acggcaacat cgatctggct gatctgcgcg cgaaagcgga acaggcgggc
     9721 gataacctct cctgtatcat ggtgacctat ccttccaccc acggtgtgta tgaagaaacg
     9781 atccgtgaag tgtgtgaagt cgtgcatcag ttcggcggtc aggtttacct tgatggcgcg
     9841 aacatgaacg cccaggttgg catcacctcg ccaggcttca ttggcgcgga cgtttcgcac
     9901 ctcaacctgc ataaaacttt ctgcattccg cacggcggtg gtggtccggg tatgggaccg
     9961 atcggtgtga aagcgcatct ggctccgttt gtaccgggtc atagcgtggt gcaaatcgaa
    10021 ggcatgttaa cccgtcaggg cgcggtttct gcggcaccgt tcggtagcgc ctctatcctg
    10081 ccaatcagct ggatgtacat ccgcatgatg ggcgcagaag ggctgaaaaa agcaagtcag
    10141 gtggcaatcc tcaacgccaa ctatattgcc agccgcctgc aggatgcctt cccggtgctg
    10201 tataccggtc gtgacggtcg cgtggcgcac gaatgtattc tcgatattcg tccgctgaaa
    10261 gaagaaaccg gcatcagcga gctggatatt gccaagcgcc tgatcgacta cggtttccac
    10321 gcgccgacca tgtcgttccc ggtggcgggt acgctgatgg ttgaaccaac tgaatctgaa
    10381 agcaaagtgg aactggatcg ctttatcgat gcgatgctgg ctatccgcgc agaaattgac
    10441 caggtgaaag ccggtgtctg gccgctggaa gataacccgt tggtgaacgc gccgcacatt
    10501 cagagcgaac tggtcgccgg gtgggcgcat ccgtacagcc gtgaagttgc ggtattcccg
    10561 gctggcgtgg cagacaaata ctggccgacg gtgaaacgtc tggatgatgt ttacggcgac
    10621 cgtaacctgt tctgttcctg cgtaccgatt agcgaatacc agtaattcac tgattcgact
    10681 ctcttctaaa ggcgcttcgg cgccttttta gcttgttaat aaagtgcgct ttgtgtgttt
    10741 cggctgaggc gtaatataaa atcttgcgag ctcaatatat taaaagagtg ccaggcctga
    10801 tgagcgtatc gcatcaggca catcggctaa ttacagattc tcgccgttgc tggcaatcac
    10861 ctctttgtac cagttaaagc tcttcttacg tgaacgcgac atatcgccag taccgtcgtc
    10921 atgtttattc acatagataa agccgtagcg tttgctgtac tgcccggtgg tgaacgacac
    10981 gcagtcgatg cagccccacg gtgtgtagcc catcagatcc acgccatcgt aagtcaccgc
    11041 ttttttcatc tcttcgatat gggcgcgcag gtagtcaatg cggtagtcat cgttgatgct
    11101 gccatcttct tccactttgt cgtaagcgcc aaaaccgttt tcgacaataa acagcggcct
    11161 ctgataacgc tcatacagtt cgcaaagggc atagcgcaga cctactggat caatctgcca
    11221 gccccagtca gacgctttaa catacgggtt cggtacgctg ccttcgaaac cagagatcgc
    11281 atcgccggtg ccgccttcgg ccttcactgc gttggtcatg taatagctga aaccaagata
    11341 atcgcaggtg ccttcgcgca gtatatcgag atcgccatct tccattttga tgttaaatcc
    11401 gcgacgctcc cactcgttca acacatagga cgggtaatag ccgcgtagct gcacatcggt
    11461 aaagacgtag cgttcgcgca tcgactcctg agcgaacatc acatcgtccg ggttacagga
    11521 gtaaggatag agcggcacca tcgccagcat acagccgact ttcatctccg ggttaatgcg
    11581 acgcgcagct ttcaccgcca gggcgctggc gacaaactgg tgatgcagca cctgatacat
    11641 cgtctcttcc gggttctcat gctcggtata aaccacgccg gagcagcagt aaccgaacag
    11701 cggtgcacgc cagttacgct ggttgttaat ttcgttgaag gtcatccagt atttgacttt
    11761 atgcttatag cgttcaaata ccacttccgc gaaacggaca aagaaatcaa ccactttacg
    11821 gttggtccag ctaccgtatt gctgcaccag atgcagcggc atttcaaagt gggagagggt
    11881 gatcactggt tcgatgttgt atttcagcaa ttcatcgaac agagagtcgt aaaacttcag
    11941 cccttcttcg tttggctgag cttcatcgcc ttttggaaaa atgcgcgtcc aggcgatgga
    12001 tgtacgaaaa catttgaagc ccatttcggc aaatagcttg atgtcttcct tgtagtgacc
    12061 ataaaaatca acggcttcat ggtttggata gtattttcct ggcaagactt ctttggtgat
    12121 ttcgcgcggc acgccgtgtg cgccaccggt cagaacgtca caaatgctcg gccctttgcc
    12181 gcctttgttc cagccgcctt cgacctgatg agcggcaact gcgccgcccc ataagaaatc
    12241 tttcggtaag gtgagttttt tcactatcat aggctccaga tgagtcaatt cttacaggaa
    12301 atagtaccat gcagccaaac gacatcactt tttttcaacg tttccaggat gacattctgg
    12361 ctgggcgtaa aaccatcacc atccgcgacg agtctgaatc gcacttcaaa acgggtgatg
    12421 tgcttcgtgt cgggcgtttt gaagatgacg gttatttttg cacgattgaa gtcaccgcaa
    12481 cctcaaccgt aacgctggat acgctgacgg aaaaacatgc agagcaggaa aatatgcccc
    12541 tgactgaact gaaaaaggtc attgccgaca tctatcccgg tcagacacag ttttatgtga
    12601 ttgaatttaa atgtctttaa attgaactca cgcctgttag ttgaaatttc gcatttaatt
    12661 tcatgatttt taggagatta gtctcaaatc agaatttatt ttagctaaca ggtgttcact
    12721 ggaactattc tcagttacgc tggagaggta tacatgtgcg tgtacgtttc tccggagtaa
    12781 gttatggttc agaagcccct cattaagcag ggatattcgc tggcagagga aattgccaac
    12841 agcgtcagtc acggcattgg gttggtgttt ggtatcgttg ggctggtgtt gctactggtt
    12901 caggcggtgg atctcaatgc cagcgccaca gcgataacca gctacagcct ctatggcggc
    12961 agtatgatcc tgctgttcct cgcttcgacg ctctatcacg ccattcctca tcaacgggca
    13021 aaaatgtggc tgaagaaatt tgaccattgc gctatttacc tgttgattgc cggaacctac
    13081 acgccgtttt tgctggtggg gctggattct ccgttagcgc gcgggttgat gattgttatc
    13141 tggagcctgg cattgctggg gattctgttc aaactgacca tcgcgcaccg attcaaaatt
    13201 ttatctctgg tgacttatct ggcgatgggc tggctgtcgc tggtggtaat ttatgaaatg
    13261 gcagttaagc tcgcggcggg cagcgttacc ttactggcgg taggcggcgt ggtttattcg
    13321 ctcggggtga ttttctacgt ctgcaaacgc attccataca accatgccat ctggcacggc
    13381 ttcgtgctcg gcggtagtgt gtgccacttt ctggcgatct atttgtatat tgggcaggcg
    13441 taatgggtat gcctttgtag gcctgataag acgcgccagc gtcgcatcag gcgataaatg
    13501 cctgatgcga cacagagatt tactcttcga gcgaatacgg cagcggctcg atatgcaatg
    13561 tattcgcatc gtcacgtacg cggaagatgc tgtccggttc catatcgtta ttcatcacta
    13621 cctgtaccac gacctgacca tcttccagtt ttaccgcagc cagcactgta ccggtacgac
    13681 gccagttctc gcccattttc agctctaagt cttcaccagc ttccggcagt cggctggcac
    13741 tacctgtcag caaccagagc gcacgtttat tggcaccacg gaattttgct cgcgccacca
    13801 tctcttgtcc ggtataacag cctttcttaa agctgatacc gcccagcgcc tggagattgg
    13861 tcgcctgtgg gataaactgc ccgctgttcg cggcatcaat caccgggaaa cccgcttcaa
    13921 tgtttaatgc cagccactgt tggctattgt tcagttccgc ttcaccgcgc agtttatcgg
    13981 tcagcatatt ggcagtagct tcatcggtta cgatcaggaa acgttctgcc gggtgttcaa
    14041 accatagcaa agtggtcgcg ccttctttaa ctacctgttt ttctctcgaa ggtagttcac
    14101 taaagagatt ggccagcgcg gcgcgcgcct gaaaaccggc aacaccaagc agcacacgct
    14161 cgtcgtctgg cgcgatggtc actttagaga acaccgcata ttttttcagt tcagtcagct
    14221 gcggttcacg cacgctgcgc cgttcaatcc acgcaaagcc atcgccgtcg cggaacagac
    14281 gtaaattgct ccacatttta cctttggcgt cgcaatgggc ggcgagcagg tgctgatttt
    14341 ctgtcatctg gctgacatct gctgtcacct ggccctgcat atatttttcg ctgtccgcgc
    14401 cagtaatggt ggcaagcgcc cagtcatcaa gcgtcatcag cgtcagcggt aaacgggcag
    14461 aagccgtagg ctgacgtgga ggaaaaggtg taaaagccat aatagagtcc tgagttgctt
    14521 aacggcgtat taataactaa tggtaaaaga gctatgaggc aatgcaagtg ctttcactac
    14581 gcgatctgtg ccttctgcga ggcgacttcc aaaaaaatga agcccttgaa ttgttgtgaa
    14641 ttaatttcgc gatcgcgatt gtgtttactg atattcccgt aaaagaagtc gaaaaacacg
    14701 ttacaatagc ggggtactgg attttcgtga gacaggaaga agatggacat taacaacaaa
    14761 gcccgcattc attgggcatg ccgccgtggt atgcgcgaac tcgatatttc aatcatgccg
    14821 tttttcgaac atgagtacga cagcttaagc gatgacgaaa aacgcatctt tattcgtctg
    14881 ctggaatgtg acgatccaga cctgtttaac tggctgatga atcacggtaa accggccgat
    14941 gcagaactgg aaatgatggt ccgactcatc cagacacgga accgggaacg tggtcctgtg
    15001 gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt tccttgctga ttcatgggct
    15061 ggttgccgct gttattttac tcatgccctg gccgctcagt tacaccccgt tatggatggt
    15121 gttactttcg ctggtggtgt ttgattgcgt tcgcagccag cggcgtatta atgctcgcca
    15181 gggggaaatt cgcttgttga tggacgggcg tttgcgttgg caagggcagg agtggagcat
    15241 cgtcaaagca ccgtggatga ttaagagcgg catgatgctg cgtttacgtt ctgatggcgg
    15301 taaacggcaa catttatggc tggcagccga cagcatggac gaagccgaat ggcgggattt
    15361 acggcggatt ttgttgcaac aagagacgca aagataaaga tgacggcaaa gcatatgcgc
    15421 taagccgtca gtgacatcag gcgtaatgct ctgccatttc gttgaggatt tgttcgcacc
    15481 agctctgaat acgctcgtca ctaaggtcat actggttagt ttcgtccagc gccagcccca
    15541 cgaacagttg cccatcagca atcaccggtt tcgggctggt aaattcatat ccttccgttg
    15601 gccagtagcc gacgaacttc acgcctttgg tcgagagttt gtcatgcagc ataccgagcg
    15661 catcgaggaa ccactcgccg tatcccagtt gatcgccaag cccatacagc gcaacaattt
    15721 taccttcgag gttcaggtcg tcgagctgat cccagacggc ttcccagtct tcctggattt
    15781 caccaaaatc ccaggtcggg atacccagaa tgagcacatc gtactgctcc attaatttcg
    15841 gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg gccgataata tcgcggattt
    15901 tttctgccgc catttcggtg taacaggtgc tggaaccgta aaaaagaccc atattcataa
    15961 cgtaagcttc tcgattctgc tgtggctttt gcggctagtg tatcagaatc gctttagtca
    16021 cggcacaata aggcataatg gatcaaatga gtgaccataa ggggcgcaag tgaaacagga
    16081 tctggcacgc atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc tggctgaaaa
    16141 tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg gagtggttgc atcaccgcgg
    16201 gttgacgctg gcgacggcgc aaagtgacga tttgcaggca ttactggcag aacggctgga
    16261 gggcgggtat aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc gattgttcca
    16321 gtatctttat cgcgaaaagt ttcgtgaaga cgatcccagt gcgcatctcg cttcaccgaa
    16381 attgccccag cgtttgccaa aagatttaag cgaagcgcag gttgaacgtt tattacaggc
    16441 accattaatt gatcagccac tggagctacg cgataaagcc atgcttgaag tgttgtatgc
    16501 taccgggctg cgtgtctctg aactggtcgg actgacaatg agtgatatca gcctgcgtca
    16561 gggcgtagtg cgggtcattg gtaaaggcaa caaagagcgc ctggtaccgt taggtgaaga
    16621 ggcggtttac tggctggaaa cctatctgga acatgggcgt ccgtggctgc tgaatggtgt
    16681 gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac agaccttctg
    16741 gcaccgtatt aaacattatg ccgtgctggc gggtatcgac agcgaaaagc tgtcaccgca
    16801 tgtgttgcgc cacgcttttg ccactcattt attaaatcat ggtgcggatt tacgcgtggt
    16861 gcagatgcta ctgggccaca gcgatctttc caccacgcaa atttatacgc atgtcgctac
    16921 cgagcgtctg cggcaacttc atcaacagca tcacccgcgg gcgtgatgtc tgaaaagaac
    16981 gggaagattt atgaagaaag gttttatgtt gtttactttg ttagcggcgt tttcaggctt
    17041 tgctcaggct gatgacgcgg caattcaaca aacgttagcc aaaatgggca tcaaaagcag
    17101 cgatattcag cccgcgcccg tagctggcat gaagacagtt ctgactaaca gcggtgtgtt
    17161 gtacatcacc gatgatggta aacatatcat tcaggggcca atgtatgacg ttagcggcac
    17221 ggctccggtc aatgtcacca ataagatgct gttaaagcag ttgaatgcgc ttgaaaaaga
    17281 gatgatcgtt tataaagcgc cgcaggaaaa acacgtcatc accgtgttta ctgatattac
    17341 ctgtggttac tgccacaaac tgcatgagca aatggcagac tacaacgcgc tggggatcac
    17401 cgtgcgttat cttgctttcc cgcgccaggg gctggatagc gatgctgaga aagaaatgaa
    17461 agctatctgg tgtgcgaaag acaaaaacaa agcgtttgat gatgtgatgg caggtaaaag
    17521 cgtcgcacca gccagttgcg acgtggatat tgccgaccat tacgcacttg gcgtccagct
    17581 tggcgttagt ggtactccgg cagttgtgct gagcaatggc acacttgttc cgggttacca
    17641 gccgccgaaa gacatgaaag aattcctcga cgaacaccaa aaaatgacca gcggtaaata
    17701 attcgcgtga aacaacagat acaacttcgt cgccgtgaag tcgatgaaac ggcagacttg
    17761 cccgctgaat tgcctccctt gctgcgccgt ttatacgcca gccggggagt acgcagtgcg
    17821 caagaactgg aacgcagtgt taaaggtatg ctgccctggc agcaactgag cggcgtcgaa
    17881 aaggccgttg agatccttta caacgctttt cgcgaaggaa cgcggattat tgtggtcggt
    17941 gatttcgacg ctgacggtgc caccagcacg gctctaagcg tgctggcgat gcgctcgctt
    18001 ggttgcagca atatcgacta cctggtacca aaccgtttcg aagacggtta cggcttaagc
    18061 ccggaagtgg tcgatcaggc ccatgcccgt ggcgcgcagt taattgtcac ggtggataac
    18121 ggtatttcct cccatgcggg ggttgagcac gctcgctcgt tgggcatccc ggttattgtt
    18181 accgatcacc atttgccagg cgacacatta cccgcagcgg aagcgatcat taaccctaac
    18241 ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc gttttatctg
    18301 atgctggcgc tgcgtacctt tttgcgcgat cagggctggt ttgatgagcg taacatcgca
    18361 attcctaacc tggcagaact gctggatctg gtcgcgctgg ggacagtagc ggacgtcgtg
    18421 ccgctagacg ctaataatcg cattctgacc tggcagggga tgagtcgcat ccgtgccgga
    18481 aagtgccgtc cggggattaa agcgctgctg gaagtggcaa accgtgatgc acaaaaactc
    18541 gccgccagcg atttaggttt tgcgctgggg ccacgtctca atgctgccgg gcgactggac
    18601 gatatgtccg tcggtgtggc gctgttgttg tgcgacaaca tcggcgaagc gcgcgtgctg
    18661 gcaaatgaac tcgatgcgct aaaccagacg cgaaaagaga tcgaacaagg aatgcaaatt
    18721 gaagccctga ccctgtgcga gaaactggag cgcagccgtg acacgctacc cggcgggctg
    18781 gcaatgtatc accccgaatg gcatcagggc gttgtcggta ttctggcttc gcgcatcaaa
    18841 gagcgttttc accgtccggt tatcgccttt gcgccagcag gtgacggtac gctgaaaggt
    18901 tccggtcgct ccattcaggg gctacatatg cgtgatgcgc tggagcgatt agacacactc
    18961 taccccggta tgatgctcaa gtttggcggt catgcgatgg cggcgggttt gtcgctggaa
    19021 gaggataaat tcgaactctt tcaacaacgg tttggcgaac tggttactga gtggctggac
    19081 ccttcgctat tgcaaggcga agtggtgtca gacggtccgt taagcccggc cgaaatgacc
    19141 atggaagtgg cgcagctgct gcgcgatgct ggcccgtggg ggcagatgtt cccggagccg
    19201 ctgtttgacg gtcatttccg tctgctgcaa cagcggctgg tgggcgaacg tcatttgaag
    19261 gtgatggtcg aaccggtcgg cggcggtcca ctgctggatg gtattgcttt taatgtcgat
    19321 accgccctct ggccggataa cggcgtgcgt gaagtgcaac tggcttataa gctcgatatc
    19381 aacgagtttc gcggcaaccg cagcctgcaa attatcatcg acaatatctg gccaatttag
    19441 cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa tcccgctctt atcaccgcat
    19501 tttgactagc tcaataaaag aaatcagacc atgtttgaaa ttaatccggt aaataatcgc
    19561 attcaggacc tcacggaacg ctccgacgtt cttagggggt atctttgact acgacgccaa
    19621 gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct ggaacgaacc
    19681 cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg tcgacaccct
    19741 cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg ctgtagaagc
    19801 tgacgacgaa gaaaccttta acgaagccgt tgctgaactc gacgccctgg aagaaaaact
    19861 ggcgcagctt gagttccgcc gtatgttctc tggcgaatat gacagcgccg actgctacct
    19921 cgatattcag gcggggtctg gcggtacaga agcacaggac tgggcgagca tgcttgagcg
    19981 tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact gaaatcatcg aagagtcgga
    20041 aggtgaagtg gcgggtatta aatccgtgac tatcaaaatc tccggcgatt acgcttacgg
    20101 ctggctgcgt acagaaaccg gcgttcaccg cctggtgcgt aaaagcccgt ttgactccgg
    20161 cggtcgtcgc catacgtcgt tcagctccgc gtttgtttac ccggaagtcg atgacgatat
    20221 tgatatcgaa atcaacccgg cggatctgcg cattgacgtt tatcgcgcgt ccggcgcggg
    20281 cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt actcacatcc caaccgggat
    20341 cgtgacccag tgccagaacg accgttccca gcacaagaac aaagaccagg ccatgaagca
    20401 gatgaaagcg aagctttatg aactggagat gcagaagaaa aatgccgaga aacaggcgat
    20461 ggaagataac aaatccgata tcggctgggg cagccagatt cgttcttatg tccttgatga
    20521 ctcccgcatt aaagatctgc gcactggggt agaaacccgc aacacgcagg ccgtgctgga
    20581 cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag gaaccaacat
    20641 gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg aactgaaaac
    20701 gcgtcgtgag aagctggcga acctgcgcga gcaggggatt gccttcccga acgatttccg
    20761 tcgcgatcat acctctgacc aattgcacgc agaattcggc ggtaaagaga acgaagaact
    20821 ggaagcgctg aatatcgaag tcgccgttgc tggccgcatg atgacccgcc gtattatggg
    20881 taaagcgtct ttcgttaccc tgcaggatgt gggtggccgc attcagctgt acgttgcccg
    20941 tgacgatctg ccggaaggcg tttacaacga gcagttcaaa aaatgggacc tcggcgacat
    21001 cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta tccactgtac
    21061 cgagctgcgt ctgctgacca aagcactgcg tccgctgccg gataaattcc acggcttgca
    21121 ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc atctctaacg atgaatcccg
    21181 taacaccttt aaagtgcgct cgcagatcct ctctggtatt cgccagttca tggtgaaccg
    21241 cggctttatg gaagttgaaa cgccgatgat gcaggtgatc ccaggcggtg ccgctgcgcg
    21301 tccgtttatc acccaccata acgcgctgga tctcgacatg tacctgcgta tcgcgccgga
    21361 actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt gtattcgaaa tcaaccgtaa
    21421 cttccgtaac gaaggtatct ccgtacgtca taacccagag ttcaccatga tggaactcta
    21481 catggcttac gcagattaca aagatctgat cgagctgacc gaatcgctgt tccgtactct
    21541 ggcacaggat attctcggta agacggaagt gacctacggc gacgtgacgc tggacttcgg
    21601 taaaccgttc gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc cggaaaccga
    21661 catggcggat ctggacaact tcgactctgc gaaagcgatt gctgaatcta tcggtatcca
    21721 cgttgagaag agctggggtc tgggccgtat cgttaccgag atcttcgaag aagtggcaga
    21781 agcacatctg attcagccga ccttcattac tgaatatccg gcagaagttt ctccgttggc
    21841 gcgtcgtaac gacgttaacc cggaaatcac agaccgcttt gagttcttca ttggtggtcg
    21901 tgaaatcggt aacggcttta gcgaactgaa tgacgcggaa gatcaggcgc agcgcttcct
    21961 ggatcaggtt gccgcgaaag atgcaggtga cgacgaagcg atgttctacg atgaagatta
    22021 cgtcaccgca ctggaacatg gcttaccgcc gacagcaggt ctggggattg gtatcgaccg
    22081 tatggtaatg ctgttcacca acagccatac catccgcgac gttattctgt tcccggcgat
    22141 gcgtccggtg aaataagcat tacgttatgc tcacaacccc ggcaaatgtc ggggtttttt
    22201 atttaagctg ggtaaatgca gataatcgta ttctggcttc gcgatttgtc gcctgcatca
    22261 ccatccacgg actgaacgcc cacggcgtgg catcaatacc gcgtaataca tcagctaaat
    22321 cacaccattg ataatccatc acttcatcat cgttgatctg taacgcactg gtggtgcgtg
    22381 cggcaaatac cggacacact tcattttcca caatgccatt cggatcggtg gcgcgatagc
    22441 gaaagtcagg atagatagat tcaggaggcg taatttccac gccaagctca taacggcaac
    22501 ggcggatcac cgcgtcttcg ttgctttctc ccagttgtgg gtgcccacaa accgggttag
    22561 tccacacgcc aggccatgct tttttgctca gtgcgcggcg ggtaactaat aattgtcctt
    22621 tggcattaaa cagccaactg gagaacgcga gatgtaagcg ggtgtctgcc gtgtgtgcgg
    22681 catacttttc cagcgtaccc gtgggaactc cctgtgcatt caataaaatg acgtgttccg
    22741 tttgcataat ttctcacatg taattctgat cactcatcat tatagataat tgaagagtga
    22801 aatgattaac caagtgagcg tttatcgaca accgcccgtt ttgagcggat gtcgacaggt
    22861 aaaaaccatt taaaaaagta gcatcatata tttgaagcga ctttcaatgg ccattgcaac
    22921 acagtcagaa atgacaggaa tagagagagt aaaaagcaac agcagactga atatagcgcg
    22981 cattaaaaaa ttcatattat tctccatgct catttttctt cagcttgcgc atcagaagcc
    23041 acatacgtac tgtcctgact tttaaatctt tatcagagac ggattccttc accaggtcag
    23101 catgatgctg gtagccatta aagaagacat tcagcacaac ggcgctaagg gttgcgagca
    23161 taatgccgct atgcagcagc ggttgcagta cggccggtaa cttagaaaag aaatcgtgag
    23221 agagcgtcgg agtcatgcca acgccgagac tgatcgccac aatatagagg ttgtaacggt
    23281 tggtggtgta gttacagcgc gacagaattc gaatccctgt cgccagtacc atgccgaaca
    23341 tcaccagccc agcgccgccc agcacaaatt gcggaatgga ggctaccagc accgccattt
    23401 ttggcaccat gccaaacagg attaatataa ttcccgaaga aatacacacc cagcggctat
    23461 gaacgcgcgt cacgctaacc aggccaacgt tttgagaaaa tgacgtatgg gggaagctgt
    23521 taaacgtgcc gcctatcatt gtccctacgc catcgacacg cagcccgcga ataatatcgt
    23581 gtgaagagag tttacgacca actatttcac ccagtgccag gaacatcccc atcgactcga
    23641 taaacacgat gattaacacg gcagtcatgg tcagaatgga aacgggatca aaaatcggca
    23701 taccaaacga catcggcgta acaatcgcaa accatgaagc atcatgtagc ccggataaat
    23761 tgacttcatt catcatccac gaaagtaaaa agccaaatac aatccccagt aatacggcga
    23821 cgttggacat aaatcctttc gcatagcgag taatgagcaa gataaaaatt aagacggcaa
    23881 aggagatacc taaataaacg ggattaccat attgcggatt ccctttacct ccggcggccc
    23941 agtcaatacc cacctgaatg atgctaagcc cgatagaagt aataaccaca ccggtaacca
    24001 gtggcggaaa taaaggcatc aagcgaccga taagtggcgc taataatgtg gtgataaaac
    24061 ccgcggcgat agtggcacca aatatcccca gcaggccgat atccgggttc atcccaatgg
    24121 ctatcattgg tgttacagca gcaaaggtca ccgacataat caccggcagg cggatcccca
    24181 taaagcggcc gataccgata cattgcaata atgtgacgat cccgcagcaa aagagatccg
    24241 agctaatgag catcgcaata gcatcttttg agaggcccag tcggtcacca atcataagag
    24301 gaacagcgac tgcacctgcg tacatgacaa ggacgtgttg cagaccgagt atgatcagct
    24361 ttcctggtga taatatgcgg tcaacctcat cagttgggcg gtcttgccca gaagatgagg
    24421 gaagttggga atctatggcg ctcatcagtg tttctccttt cgcccgccag agtaaaaagc
    24481 aaccgtttac gttaatgaaa ctgtctaaac acatgctcgt ggagtgccag tttttgcgag
    24541 gaaggggagt tagagacagg aattgcggag gcaatcacat aatcgtgctt aaaccacgaa
    24601 aatgagaaga gaacctgaca atggaattat ctgtaatttt ctcatattga tatttcatta
    24661 atcattctgg catgtcgcac tctcgcattt aatcgttttt atctggatag cgctcttttg
    24721 atcggcaatg ttgaaataat gaaagcggca tagattgagg ggcaataatt tgaactcagt
    24781 cgcaaaatta taattaaaac ggtcatgcta cctaatatat gcccgtttat tgcatcgcga
    24841 aatttgagaa gacataactc attaaatgaa agggatgcaa atgaataagt ttatcgctgc
    24901 tgaagctgcg gaatgtatag gctgtcatgc ttgtgaaatt gcctgtgcgg tggcacacaa
    24961 tcaagaaaac tggccgctga gtcacagtga ctttcgacca cgtatccaca ttgtagggaa
    25021 aggccaggct gcgaatccgg tggcctgcca tcactgcaac aatgcccctt gcgttacggc
    25081 ttgtccggtt aatgctctga ctttccagtc cgatagcgta caactggacg agcaaaaatg
    25141 tattggttgt aaaagatgcg caatcgcttg cccctttggc gtcgttgaga tggtcgatac
    25201 gattgcgcag aaatgcgacc tttgtaacca gcgcagttcc ggcacgcaag cctgtattga
    25261 agtttgccca acacaggcgt tacgactgat ggacgataaa gggttacagc agataaaggt
    25321 ggcccgccag cgcaaaacgg cagcaggaaa agcgtcatca gacgctcagc catctcgcag
    25381 tgcagcgttg ctccccgtta actcgcgtaa aggcgcagat aaaatttcag caagtgaacg
    25441 gaaaaaccat tttggcgaaa tctattgcgg gctggatcca caacaagcga cttatgagag
    25501 tgaccgctgt gtttattgtg ccgaaaaagc taactgcaac tggcattgcc cgctgcataa
    25561 cgctattccg gattacatcc gtctggtaca ggagggaaag attattgaag cggcagaact
    25621 ttgccaccag accagttcct taccagaaat ctgcggcagg gtatgtccac aagaccgtct
    25681 ttgtgaaggc gcatgtactt tgaaagatca ctctggcgca gtctctatcg gtaatctgga
    25741 acgctacatc accgataccg cgctggcgat gggctggcgt cccgatgtca gcaaagttgt
    25801 tccccgtatc gagaaagtgg cggtgattgg cgctgggcct gcagggttag ggtgtgctga
    25861 tattctggcg cgcgcaggag ttcaggtcga tgtctttgat cgccatccag aaattggcgg
    25921 tatgctgact tttggcattc ctcctttcaa actcgataaa acggtattaa gccagcggcg
    25981 agagatattc accgcaatgg gaatcgactt tcatcttaac tgtgaaattg gctgcgatat
    26041 tacctttagc gatttaactt ctgaatatga tgcagttttc atcggcgtgg ggacttacgg
    26101 gatgatgcga gctgatctgc cgcatgaaga tgcgcccggt gtcattcagg ctctaccgtt
    26161 cttgactgcc catacccgcc agctcatggg gttgccggag tctgaagagt atccgctgac
    26221 ggacgtggaa ggtaagcgag tcgtggtatt gggcggtggc gatacgacaa tggattgttt
    26281 gcggacttcc atccgcctca atgccgccag cgtgacctgc gcgtatcgtc gtgatgaagt
    26341 cagtatgccg ggctcgcgca aagaggtggt caatgcgcgc gaggaaggtg tcgagtttca
    26401 attcaatgtt cagccgcaat atatcgcttg tgacgaagat gggcgtttaa ctgcggtggg
    26461 cctgattcgt accgccatgg gtgagccggg gccggatggt cgccgtcgtc ctcgtccggt
    26521 tgcgggttca gagtttgaat tgcccgccga tgttctcatt atggcctttg gtttccaggc
    26581 gcatgccatg ccgtggttgc agggcagcgg aattaaactc gataaatggg gcctgattca
    26641 aaccggcgac gttgggtatt tacctaccca gacgcatctg aaaaaagtct ttgctggtgg
    26701 ggatgcagtt catggcgcgg atctggttgt cactgccatg gccgcaggaa ggcaggcggc
    26761 gcgcgatatg ttaactctgt ttgatacgaa ggcatcgtga tgaaatcgtt aattatcgtt
    26821 aatccggctg actgtattgg ctgccgtact tgtgaagtgg cctgtgtggt cgctcaccct
    26881 tcagaacagg agttgaatgc cgatatcttt ttaccccggc tgaaggtaca gcgactggac
    26941 tgcatcagtg cgccggtgat gtgccaccaa tgtgaaaacg ccccttgtgt tggcgcttgc
    27001 cccgtggggg cgctgacgat gggcgagcag gtggtgcaga ccaattctgc ccgttgtatt
    27061 ggctgtcaga gttgcgtcag tgcgtgcccg tttggaatga tcaccattca gtcgttgctg
    27121 ggggataccc ggcaacaaat cgtgaaatgc gatctctgtg aacagcggga agaggggcct
    27181 gcctgcgttg aatcttgccc aacacaggcg ctgcagttgc ttactgaaag agaactcagg
    27241 cgagtccgcc agcagcgtat tgttgccagc ggtgagaatc ttctctgaaa gaaatctccg
    27301 cgctgaaagg cgcggagaat ctattagatt gcccaaccac ccgcgtaaaa tgcgaccagt
    27361 gcggcagtaa tgatcaccgt accaatattc agcttttgcc attcgcgtgc aaagacgcgg
    27421 cctacgacca gtgtcacaaa gcccagcata ataccggtaa cgatattaca agtcagaacg
    27481 atgaacacgg cacacaccag gccagccata gcgtcaataa aatcattgaa atccagcttc
    27541 gagacgttac ttaacatcag caaacctacg tacatcagag cgggtgcagt ggcgtaacca
    27601 gggatcaaaa atgacagcgg tgataagaac agaatcagca ggaataacgc ccccactacg
    27661 gttgcggtta accctgtttt acccccggcg gcggttcctg ccgctgattc gatataaacc
    27721 gctgcgggcg ctgcgcccac caggccggag aatattgaac ttacagagtc actggtcagg
    27781 gctttgccgc cgttgatgat ctggttgtct ttatccaaca aattcgcctg accggcgacg
    27841 gcacggatgg tgccagtagc gtcgaacact gcggtcatca ccaatgccag tacactcgga
    27901 agtacagttg gctggagtgc gcccataata tcgaggctga aaatcagaga cttaccatct
    27961 tcgccagtca ggcttggcat cgccaccaga ccgtggtatt tcaccgctgg gtcaaagatt
    28021 aagccgatga tcgaaattgc aataatcacc aacaagatcc cgccgggtac gcgacacttc
    28081 tccagaccga agatgaccgc cagccccagc aagctcatca tcaccgggaa ggaggtaaac
    28141 gcaccgagcg ccactggcaa gccttcaatc gggtttttga taaccatacc cacaccgtta
    28201 gcagcaatca gcagcagaaa cagcccgata ccgatacctg taccgtgagc gatacccatc
    28261 ggcaaattac gtaagatcca ggtacgcaca ccggttacgg aaatggcggt gaagatgacg
    28321 cccatcagaa ataccgcgcc cagtgcgaca ggaacgctaa tttgttgccc gagtaccaga
    28381 ctgaatgcgg taaacgccgt caaggaaatc gcgcaaccaa tcgccattgg caaattagcc
    28441 cataacccca tcagcaacga gccgaagccc gcgaccagac aggtggcaac aaacacagct
    28501 gcgggaggaa aacctgcttt gcccagcatt cccggaacga cgataacgga ataaaccatg
    28561 gccagaaagg tcgttaagcc agccagtact tcctgacgaa cagtactgcc acgagcggta
    28621 attttaaaat aattatccag cgcgccctgt ggctttggtg cgtccggtgt ttgtaggatg
    28681 tctccagaca taatgatgtc ctcacgagga tgttaaagtt ttattattag ttgcgttcgt
    28741 acaccagacg accatcaacg taggtgcggt agatcgaacg gtcatcgccc aacgtcatca
    28801 tcacgaacaa tttgtcgact aaagaaacag agttgtcata gcgcagctgc tgtagcggag
    28861 tggcggtggg ttccatcacc acgaaatcag cctctttgcc aggtaaaaag ttgccaatca
    28921 aatcgtcaag gcccagagat ttcgctccgc cgagcgtggc caggtaaaac gcttcatatg
    28981 ccgagaggcg atagccttgt aattgcaaca ctttgtaggc ttcgttcagc gtttgcagca
    29041 tgttgaaagt ggttccggca ccgatatccg ttcccatgcc cactttaact ttcttctgcc
    29101 atgctttttt caagttgaat aacccgctgc ccaggtaaag gttggaggtc ggacagaaag
    29161 caatgctgga tttggtttcg ctgagacgat cccactcttt ttcttcgaga tggacgcagt
    29221 gagcaaagac acagttttta ccggtcaggc cgtactgatg gtaaacatcc agataaccat
    29281 catggtcagg ataaagcgat ttcacccagg caatttcatc tttgttttca cagagatggg
    29341 tatgtaccca cgtatccgga tattcctctt tcaggcgttg cgccatcgcc atctgttcag
    29401 gagatgaggt aggggcgaat cgtggcgtaa tcgcatacaa cagacgacca tttttgtgcc
    29461 agcgttcgat cagttctttg ctttggtgat agctgctttc ggcagtgtcg agcagataat
    29521 ccggtgcgtt acggtccatc atcaccttac cggcaatcat acgcatattg atatgactgg
    29581 cggcttcaaa cagcgcatca acggattgcg gatgaacagt gccaaacacc agcgccgtgg
    29641 tggttccgtt acgtaaaagc tgcttgatga agaacgccga catttcgcgg gcgtactcta
    29701 aatcctcata acgacgttca gtagggaagg tgtgtttatt caaccactcc agcaattgct
    29761 caccataggc ccccaccatt tcactttgcg gataatggat atgtgtatcg acaaagcccg
    29821 gtactatcag tttgccgcga tagtcgcgca cgcgaatggt gtcaggaatt tgatgctttc
    29881 cgttttccca ttcgccaaac cattccactt ttccctgttt aatgagtaat aaaccatcct
    29941 caataaaccg cagcgcagag gcaatctctt ccgggttatc gaccgtacgg gtgacatcaa
    30001 taaaactgcc tcgtaccgct tttaacgtgt gttctcctga catcattgac tcctttactg
    30061 ttaatccatc tcttccgctg aggtaatacc aggcagaacg ttttcctgtc ggtagccacc
    30121 agggaggata atatttaata aaatcgcagt taacccgcca gcacaaatag ggttttcaac
    30181 taatacataa atagaggctg gcaatatttt aaaaatttcg ggatcgtagg agacgccaag
    30241 ccctaaacct aaagaagtgg cgacaataag tgtttcacgc cgctttaatc cgttggtgat
    30301 gatgatgcga atcccagcga tggcaatcat ggaaaacatc aacgtcattg cgcctcccag
    30361 aactgccgag ggaatggtcg tgaagaagcc gccaatcatc ggaaataagc cgaggataac
    30421 cagcattacc gcgatggttc gcccgacata acgtgaagcg acgccagtca tctgaataac
    30481 cccattattt tgcgcaaacg tggttaatgg taatgaacca acagcggagg cgataacaga
    30541 aaccagacca tctgccagca cgccgccttt cagtcgggac tgatactctt ccccctgaat
    30601 ggggcggcgg gaaaccattg ccgtggcggt gatatcgccg actgcttcca gcacgctaag
    30661 cagataaatc gtgccaacca ccaggaactg atggaagcta aaactaaagc cgtatttgaa
    30721 cggatgcggg atggtgatta acggcaaatt gcgcatactg ctgaaatcca ccatgcccag
    30781 gcataacgat gcgatatagc cgacacatag cccaatggcg atccctccca tgcgtagcaa
    30841 cggactgcga cagcagttaa agccgatcac cacaattaaa accaataaac caacgccgag
    30901 atgttcgtaa ttgccgaacg taccgctgct tttggctgca aatcctccac caaaatcgat
    30961 aatgccgact ttaatcaggc ttaagccgat catcagtacc acaataccgc tgacggtagg
    31021 cgtaataacc cgacgtaaat agggcaagat aaatgaagat ccgacaacca gaaatgcgcc
    31081 aacgaaggag acgccgagaa gcgacgacat gattaactct tcgtgaaaac cgtcgctttt
    31141 catgctgctg cccagcgcaa tcatgaccgt aacaaatgaa aaattgactg actgaattga
    31201 gagtaggcca gaaccgacga tgccgtagcg gtttacttgt aaccaggtac caataccaga
    31261 ggcgatcatc gccatagaaa caagataggc ggttgtttca gcggaaagct gtaaggccgc
    31321 acccacgatt aacgcggggg ttaccatcgg aacgaaaatt gccaacagat gggtaatggc
    31381 accaacgaga gcctgatgaa agggagggcg atcctccagt tcaaatataa ggtcagaacc
    31441 tgcatggttt atatcagaca tcccttcact ccttttaaag taaatttgat catctgaatt
    31501 agtaaaataa attcccacga atttggggcg caggacgcct tgagaacggc ttatctcatt
    31561 ttcgcagtca ctatttagtc atcgttcaaa cagagaaaca ggccgccgaa gcggccattt
    31621 attacttata ttttttccag cgcggtgagt attttctccg gtgtgaaatg ccattcgcgt
    31681 aaccagatgc cgcaggcatc gtgaattgcg gtagcaatcg ccggagctgc gccatttaca
    31741 ccaatttccg agatcgattt cgccccgaac gggccgactt tatcgtcgct cggcaccaac
    31801 acagcgcgga aatcacgcgg aatgtcacca attttcggtg cgccataact gcgtaaatca
    31861 cgcgttaacg ggtgaccttc ggcgtcataa atgatctctt cgctcatact gtggccgata
    31921 gcacgcaggg tggcaccgta gatttgtccc aacgctaact ctggattgac cggtgtaccg
    31981 cagtccagca aggcgtagaa tttatccagg cggatttcac ccgtacgcgt gttgacggca
    32041 acttcagcga agtttgcgcc atacgggaaa gcgaaatcag gcgtgatata actgccagtt
    32101 cccaccagtg aaccaaagcc ggtgccggtt tcgcctttat gggcaatatc cccgaaacta
    32161 acttcgcctt tcttgccgcg cacgacgccc ggcgttgcta gttgaacatc tgccactggc
    32221 tcacccaaca tttgcgcgcc gtggaacaga attttctccc gcagattttc cgctgccaaa
    32281 cgcgccgcgt tacccgagaa gcaagtaccg gacgaggcat atgcgccttt atcaaacaac
    32341 gcatgatcgg tatcaccgga gataacatgc acgtcctgcg gtgggcagtg cagcacttct
    32401 gctgccagtt tcgtcactac agtatccaga ccagtaccaa tatccgcacc gccagaatga
    32461 acgataaagg tgccgtccga ttccagtttg atcatgcagt tagcctgatc gatatccggg
    32521 atacccgatt tctgcatgat aatggcgacg ccgcgaccga tgtgccagtc accattttgc
    32581 ggtttcggtg aagaccattg gatcatctcg cgaccctgac gcaggatctc ttccagtgcg
    32641 cagctggcgg cggaaggaac ggaggttggc gctttacctt caccaattgc accgagaatt
    32701 ttcagctctt gcccttcgtg tacccggtta cgttcgataa tttccagttg gtcgatctgt
    32761 aactgttcag caagttccgc taatgccatg gtgatagcga agttaccttt cggtgcgcca
    32821 taaccctgat aagcaccatt tgggcaaatg ttgctgtagt aggtggtgac ctggaaatcg
    32881 acgttatcgc acggatataa gggcagcgac agcgccggtc cgttacacgg tacggtgagt
    32941 gagtggttgc cgtaagggcc agtgttggcg cggaaatcca tcttcactgc cgtcaggcga
    33001 ccatcttttt tcgctcccag tttgacggtg actttcgcga cgtgacgaga ggtgttagca
    33061 ataaactctt cttcacgggt gtagcggaac agtaccggac gcccggtcac gcaggttgcc
    33121 catgcgcaca cttcttccag caggatgtcc tgtttggaac caaaaccgcc gccaactcgc
    33181 tctttaatga catgaacttt atgctgtttc atgcccacga ggcgcgcgac ctggcggcgt
    33241 aagtgccatg gtacctgggt ggaggcgtgg ataaccagac gatcgccatc catgcgggta
    33301 aagcagatat gtgtttcagt cgggcactgc tgcgcctgcg ttgagttata ggttcgctca
    33361 atgatcacat cggcatcggc aaagcctttg tccatatcgc caatatgacc atgaatactg
    33421 gcggcgatat ttttgcgggg gcgagaaccg atcgggaagt taatgatcat atgctcgccg
    33481 cgctgggctg catggctgtt atcgtcttcc agagtatctg gcgcaccagc aacatacacc
    33541 accggttcat cgtgcacgac aggcgcatct tccgccattg cttcgtcgat cgacattacc
    33601 ggcttaagca cttcatattc aacgtcgata agcttcagtg cttcgagcgc aatttcttca
    33661 ctttctgcga ccaccgcagc aacacgatcg ccgacgtgac gcattttctt gccgaacata
    33721 cggcggtcaa gcggtgacgg ttccggtgcg ctctgccccc ccggggtata gtagatatcc
    33781 gggcaattca gatgagtaat aacgtgaacg acgcccggta aggcttcagc tttgctgaca
    33841 tccagatgag taatcagtgc gtgagcgtgt gggctacgta acattttaat gacgcaggcg
    33901 tcagccgtta cgcggtcttc aacatagcag ggcttcgcct gcaccatttt tgcggcatca
    33961 gttttaggat aatgcttacc aatgacttca aggtcgtcac ggaaagtcgg agcgatatca
    34021 atggtggcct gcggattatt tttacgtgca accgccagtt caatgacctg atagtattgc
    34081 tgccagccag catcgcggct aaacagacct gagagcgcgt cgtcgatctc ttcgcgggta
    34141 ggtgcggcga tgcgatcgag aagatcggtg ataatcagag ctgcagctgg atcgttataa
    34201 ccagactgca ccacgccaac atcaaccatt gcctgttgaa ccagacttaa ctcgttccat
    34261 ttgcccagag attctgcggt acgaatatct gccttctcta actgtgcggc aataagcaag
    34321 gacgcgttaa cgatattacc gttaaagata attgcgtcag aaccggcaaa cccgaagcca
    34381 tcatcactgt tgcgtacaga gtgcattccc atgttaaaca acagcttttg cacgttttcg
    34441 cctggattaa cggttagctc ctgaggcgcg ccatttaaag taaagtggat gatcatacgg
    34501 cttcctcccc agcctgttgg cagtcggcat acaggtcggc taccagaact cccgtgatat
    34561 agcgtttata ggccacgctg ccccgcaaat cttcctgcgg gaaaatggcg ttggcgacag
    34621 cctgttcaag tgcattgcct tccagatttt gtttttcgac atcgtgcaga cgcagtgctt
    34681 tactggcgac gccatccagc gcaattcgca taccgtcgtg gtctgtcatt gcaacggctg
    34741 ccgtcacgac ggttaaacct gcctgagaac ggctaatttt ccgggtcgca caggtgcgat
    34801 acggatcttt aatgataatt tcggttagca ggcgatcgca tgggcaggcc aggtagtcct
    34861 cgattgacag cgtttcgccg ttgccaaaaa ccagttcagc atccagtgcc agcaggacgg
    34921 gaagcagcac cgactcttcc tggcgggcgg cgatttcacc accaatggtc gactgattac
    34981 gaacatggcg tgagtaaaca aaaccgaggg cttcacgcag cgctgcagga ataaatcgcg
    35041 catcacgcag tggctgcaag cgagacattg cgccaatccg cagtgcaccg ttatcccagt
    35101 caacccagtc cagttccaga tcctgtaagg agatggcaat ctttttatcg gtacgggttg
    35161 gtgtagcatt gagtttgctg cccccggcga accagacggc ttcatcctgg tagcggcgct
    35221 tcagttccag cgcctgttcg acagagtcgg gcctgaaaaa ttgttcaatc atcttctttc
    35281 cttatttaac cctcttccct gcgggaagag ggcatctgtc atttatgcca gcgcatccat
    35341 ccgacgccac atactggcag cggcttttct ggcttgtgca taaatggaat cgcaatcgaa
    35401 gttaaactga cggtcttcat agaccatcac accattgacc atcacgctgt gaacgctgcc
    35461 tgagcccata ccgaaagcga tatgcccggc gatattgtct gccagcagcg gcgtcggcga
    35521 gttgtaatcg caaatggtta aatcagcttt gtaaccggct ttcagaagcc caaatttcgc
    35581 gccaaagttg cggctcatca gttcgttacc gttagtcagg gctttggcaa aactgtcagg
    35641 ccacagcgga ccacccgcat cgcgatgttt aaagaaggca aatttcatct cttcaaacat
    35701 gtccgaacca atgccgtccg ttcccaacgc cagattgcgg atgtcgctaa ggtgatggtt
    35761 gtagccgaca tggttgttca tgtttgaacg ggcgttatgc accaggaacg catcgcgctg
    35821 attgagtagg gtgatgtcat ctttcgacaa gtacagccca tgagcgacca gtgttttgct
    35881 gtcgatgaga tcgaattgcg ccagtcgtgc cagcaggtct ttgccgtacc agtggtgact
    35941 gtaggaaacg tcgtaaaggt cttccgcagc gtgaatatgc aaaccacggc ctgtggcttt
    36001 cacggcttca cgcagcatct ccagaccggc atctggcacg gtaaacggcg cgtgagcacc
    36061 gatatgtgct tccaccagat acggctcggt tgctgctttc ttcgcctcat caatctgacg
    36121 ggcaaagcgg atgttttctt ctacaccttc ctgcaactct ttgatgccgt tgttacggtc
    36181 agtagtttca aaacaggtca tcgcgcgcag gccaactttt aaaaatgcgt cgcgcaatgt
    36241 ggagagcgac ccgccgatat acgccggaga ggcatggtga tcgataaccg atgtacatcc
    36301 gctcttaatc gcttccaggg aacaaatcag tccgctgtaa tagagcgact cttcatcaag
    36361 ggcgcgatcg agccgccacc agagattttt cagcgttgag atgaaatccg ggcaaggggc
    36421 gatgtttgcc ataattccgc gggaaagccc cgagtaaaaa tggttgtgcg agcagacaat
    36481 tcccggcatc acaatccgac catgcatctc tttgaagctg gcgtcggggt agcgttgcgt
    36541 cagggcatcg ccgatagcga caatcacatc gttttcgatg gcgatatcaa cgccttcctg
    36601 cacttttgcc gggtgtagct gtaccgcagt gacattcttc agaatcaaca tgattacacc
    36661 tccacgcggc ccagcagata atgatgatgc tggtggacat ggctgatgat gcggcacata
    36721 tcgttcagct ccggtggtac gttgttaaac tgacctttgc tgtcgatgtt taacacccag
    36781 ctttggttat tcagacgtac tcgtacccgg caatcttcca caaggaagcc tgggttgctg
    36841 ctgttatcaa agtcttgcgc caggctgaag acggtgattt tgtctttgta cggtttaccg
    36901 ttccacggac agaactgagc gcagttgccg cattcgttac agtaagcgtc gaggtgcagc
    36961 gtctggaaac ggttctggaa gcctgggacc gcaatggaga cgttggcgcg gttcgggcag
    37021 acatccacac acttgctgca aacgtagtta cattcgaggc agcgagcggc ttcctgggcg
    37081 acaaacgcgt cacgatcgtc actgttcacc agcgtgatag agatatcgcc tttacgttga
    37141 tagatttccg ccggattgac gttgttccag tatttatcgt tctggtggga acggatattt
    37201 tcccgactaa ggatggcatc ggtcgcccga cgcgcggttc cgacagcagc gacaatggag
    37261 gatggtccgc gctgtacgtc gccgatcata aagacgtcag tcagacgagt ttcgccatta
    37321 tggtcgacgt ctggccagcc gtttttgtcc agcggcacgc ccatcgcatt cagggcttca
    37381 gtatcctgct gttcaccaat ggcggtgatc aggctgtcta catgcagtgt tactgtttcg
    37441 ttggtttcaa ccggacgacg acgacctttc tcatccggtt cgccaagcga cataacgcgc
    37501 aaggttaagg tgccatcagc atcgaaacgt tccggattgt tcaggaaacg gaactccacg
    37561 ccgtcgtgca acgcttcttc atactcttcg cgccatgcgg gcatctcttg cagtgaacga
    37621 cggtaaacga tcgttgcttt ttctacgcct ggaacgcgta acgccgcacg agcgcagtcc
    37681 attgcggtgt tacccgcccc gacaacgacc acatgtttgc ccagcttgag cgctgtaccc
    37741 ttgttgtatt cacgcaggaa ggggagtgat ttccagacat tttggttgtc gcccgccagt
    37801 ttcacaccgc tatttttatc agtgccggtg gcaatcagaa catagtggaa gccctgattt
    37861 tttaactgtt caacggttaa atcgggtgag cagccatact caaatttcac gccgtgatcg
    37921 gcaacaaaat cgatatcgtg ctgaattaac tctgcaggaa tacggaactg aggaatgata
    37981 tttttcacta cgccgcccgc attggcttcg cgttcaaaca gcgtaaccgg atggcccgct
    38041 ctggcaagga agtaacctgc agccagaccc gccggacctg caccaatcac ggcaaccgga
    38101 tggcgtgaac cagaaccggc tggtttgtgc cagcgttgct tatattcatc ccaacctttt
    38161 tccagcgcga cttttttcag ttcgcggata ttcagcgcac tgtcgtaatc caggcgggta
    38221 cagttgtatt ggcactggtg atcgcaaata tgaccggtaa tggcaggtag ggcgttgcgt
    38281 tggtagatga gttccagcgc gtcggcatag cggtgttcgc caagcagacg gatgtattcc
    38341 ggaatatctt gcttgatagc gcaggcagta acacaggggg caacgtagca gtcggtcagc
    38401 ggcaggtctt ctgccacttc aatacgctct tctggcttcc agtgtttctg ggtgtattcc
    38461 atggttaacg cgtctgctgc cagtctgttc agtcgttcga cgtcaacatg gtcaagtccc
    38521 caggcgtcgg agccttccag ctcgcgcatg caggcactta agcgcagata gccgccaggt
    38581 ttcagcagat cggttgccat agtaataggg cgaataccag tatcaaaaat atcgcggata
    38641 gtcagctgac tggcaccacc ggaataagaa atgggcagtt tgccgtcaaa ggcgcgagag
    38701 agaactgctg caacattgat ggagagcggg aacagcgcac ggcctgacat atacatctct
    38761 tcaccgggca gtgcgccttt attgttgatg gtgccgagag tgttagtcag ttttacgcca
    38821 aagccgagtg atttttcttt tgccagtgcc atcaggcgtt ccagcatttc cagtgcttgc
    38881 gtcagcttga ggtcgtgatc aaatgactct tcttttaagc ctatgtaacc gaaaccgcag
    38941 acatcgagga tctcacgaac acgcgcgtac ccgagtaagg tcgggttaag tttgacaaag
    39001 gtgttgagcc ctttttcttc cagcatgtag cggcaaatgg cttcgatttc atgcggagga
    39061 cagccgtgca tggtggagag ggtgacgccc tgcaccatac tggtggggat gcgagcgggt
    39121 aaagcttgca agctttcgcg tttttcctgc aatccgtggc gggctaaaaa tgcgtcatcc
    39181 tggagtaatt tattcagcgt atcgcgatat tgcgcgaatt tcggatggtc agatgcgtcc
    39241 atcatattgt cgatgaactg ttgcatcggc ggttgcttaa taccttcaag gttgtaaccg
    39301 acgctcatat taaagatgaa cgatttaccg gaatcagaag gctggaacat cgcttcgaga
    39361 aggtgcaggg caaaccaggc tttgaggtat tcatcccagg ctttaagcag ggtaaattcg
    39421 gtagaccatt cggtgttaaa gcactcgtct tcggcatcga tacaaggctt ttccagctcc
    39481 aggcggtcaa gaatttggac ggtttttagt tcgatgaagc gtccgccagt cagccaggaa
    39541 gtgacaatat tttgcgcgag ctgcgtgtgc gggccagcgg cagggccgac gggagtggca
    39601 caggtttcac cgaagacgct aacagtttta ccttttacgg gtgagtaaaa ctgttgctcg
    39661 ggaataccaa agattgagcg ttgttgttgg tattcatcaa atatgcgcgt caaaagttcc
    39721 tcaaacggaa tgggacgcat aatatccccc ataaccctct ccttagctta tacagatatc
    39781 attgagtgac cggtgttgag aaatccggtg gtttaggggg atagagcaac aatcgcacca
    39841 ctcttgccag gtttggcggg aaatataaaa aaatgtgagg cggctcgaaa cttaagggct
    39901 ttttgtgaat gaactcacag gtgccaaatg agaattaaaa tcactcctga aaattattat
    39961 tgccagccca tttatcactt tttctctttt ctcaaaatta gtgattcctt atcattatga
    40021 taaagagtgc tggaggggtt aaatatttga acgcaatcgt ttaactacgt tttctaaatt
    40081 tcagtatacc ttttcttcgc tgtaataaag tcgtccggag tatcaatatc taaaattatt
    40141 tctgcatttt ctatttcgac ggaataatga tctcccataa ggagtgcctg acgcatattg
    40201 gtgacattgg gtcgctggaa tgcctgcatc aagcatggtt ttgacactaa aatcggatgg
    40261 ccggggatgc cattatggag tggcagtatt gcgccatcat ttcgtaacga ccagattttt
    40321 ctaaaaatat caatggtgag ggttggcatg tcaccgtggg tgagaaaaca atgttctgtt
    40381 tgcactgcgg gtactgcggc cttgactgat gttagtaaac cctgggcata atctgggttg
    40441 tgaataatag tgatattact ctggttcgca taacgttcgt gcagttcatt accacgatag
    40501 ccggtgacta aaataattcg gctacaaaac tgcaacgcat ttttgatact tgtatcaaga
    40561 attgttccct gttgccaggg taacatcatt ttccattgcc ccattcttga tgataatccg
    40621 gcagcggtaa ttatacagtc gatggctgac atagtggttc cattatttat aggggctaat
    40681 aacgcctgat aattgcgtta cttcctgatg gattaagttg ggggtaattt atcccagaga
    40741 ggtcataaag actcgtgaaa agtatagttg acccatcggc attattcatt gatttaggtg
    40801 cgcagaaacg tcccactgtg atttctgttg ttggtgctgg ggggaaaacc agcttgcttt
    40861 tttggctggc agaactgctc cagactcgcg gcaggcgtgt gttaatcact acgacgacac
    40921 atatgtttat gccaaagtca cactggcccg tggttttctg tcgtgatccc gccatgcttc
    40981 ctcatgcgtc tcttacatcc cccatttcat tttgttttca cagctggaaa gcgaaccagg
    41041 gaaaagtgca gggatttacg ccagaagcga ttgatgcact ggtgcaacga ccagagtgtg
    41101 acgtaattct cattgaagca gatggctcgc gtggaatgcc gttaaaagcg cctgatgagc
    41161 acgaaccttg catacccaaa agcagttgct gcgtgattgc tgtgatggga ggacatactt
    41221 tgggcgcgaa agtgagcaca gaaaatgtcc atcgctggtc gcagtttgct gatattaccg
    41281 ggttaacacc tgatgcaacc ttgcaactga gcgatctcgt tgcgctggtt cgccaccctc
    41341 agggggcgtt taaaaacgta ccgcaaggtt gtcggcgagt ctggttcatt aaccgttttt
    41401 ctcaatgtga gaatgcgatt gcacaaagcg agctccttca accgctgcaa caacatgacg
    41461 tagaggcaat ctggctgggc gatatacaag aacatcctgc aatcgcgcgc agatttgtga
    41521 attagcggcc cgggtattcc gggttactga aaggatatcc attgaggttg gtatgaatat
    41581 tttcacagag gctgcaaaac tcgaagagca aaattgtccg tttgcgatgg cgcaaattgt
    41641 tgatagccga ggctcgactc cccgccattc tgcacaaatg ttagtgcgcg ccgatggttc
    41701 tatcgtcggt acaattggtg gcggaatggt tgagcggaag gtgattgaag agtcgcttca
    41761 ggcattgcag gaacgtaagc cgcgattatt ccatggacgt atggctcgta acggtgcgga
    41821 tgctgtcggg tcagattgtg gaggtgcaat gtcagtattt atcagcgtcc atggtatgcg
    41881 tccacgtctg gtgttgattg gcgcggggca tgtcaaccgg gcgatagccc agagtgcggc
    41941 gctattagga tttgatattg ccgttgctga tatttatcgc gaaagcctca atcctgaact
    42001 attcccacca tcaaccacgc ttctccatgc tgagtcgttt ggtgcggcag tggaagcact
    42061 ggatattcgc cctgataatt ttgtcctgat tgccacgaat aatcaggatc gtgaagccct
    42121 cgacaaactc attgaacagc ccattgcatg gttggggttg ctggcaagtc gtcgcaaggt
    42181 tcagcttttc ctgcgtcaat tgcgtgagaa aggcgtggct gaagaacata ttgcccgttt
    42241 acatgcgccc gttggttaca acataggtgc ggaaacgccg caggagatcg ccatcagcgt
    42301 gctggcagaa atattacagg tgaaaaataa cgcgccgggt gggctgatga tgaaaccttc
    42361 tcatccttcc ggacaccagc tggtggtgat tcgcggtgcg ggggatatcg ccagtggtgt
    42421 ggcgctacgt ctgtatcatg cgggttttaa agtgatcatg ctggaagtgg aaaaaccgac
    42481 agtgattcgt tgtaccgtgg cgtttgccca ggccgtgttc gatggcgaaa tgacggtcga
    42541 aggcgtcact gctcgcctga caaccagctc tgcggaagcg atgaaactta ccgaacgcgg
    42601 attcatccct gtgatggtag atcccgcctg ttcattgctt gatgaactga aaccgctttg
    42661 cgtggtggac gctattctgg cgaaacagaa tttgggaacg cgggcagata tggcaccagt
    42721 aacaatcgcg cttgggcctg gctttactgc tgggaaggat tgtcatgcgg taattgaaac
    42781 aaatcgcggg cactggctcg gtcaggtgat ttactctggt tgtgcgcagg agaataccgg
    42841 tgttcccggc aatattatgg ggcataccac ccgacgggtg atccgtgctc ctgctgcagg
    42901 cattatgcga tccaacgtga aattaggcga tctggtgaaa gagggcgatg tgattgcctg
    42961 gattggtgag catgaaatta aagcaccgtt gacggggatg gtgcgtggct tgttgaacga
    43021 cggactggca gtggtcggtg gttttaaaat tggtgatatc gatcctcgtg gtgaaacggc
    43081 tgatttcacc agcgtttctg ataaagcccg ggcgattggc ggcggcgtac ttgaagcgtt
    43141 aatgatgttg atgcatcagg gcgttaaagc gacgaaagaa gtgctggaag tggcttaaat
    43201 gaaaatggcc ggatggtatt acccacccgg cctgagactg atgacaaacg ccaaattgcc
    43261 tgatgcgcta cgcttatcag gcctacaagt gcattgcaat ctattgaatt tgcgatgttt
    43321 tgtaggacgg ataaggcgct cgcgccgcat ccagcatcaa caaagcgcac ttacttgaca
    43381 atctttggcc ggatggtatt acccacccgg ccttaaaatt actgacaaat caccgttccg
    43441 gttttgcctt caatcccttc tttcgctttg ctcaatacgg taatcagtgc ttcacgacct
    43501 gcgcgagagc gagcaaacga ggcggcagct tccactttag gcagcataga acctttcgca
    43561 aaatgacctt cttcgataaa gcgttcagca tcacttaatg acaggcgatc gagccactgt
    43621 tcattctctt taccgaagtt aatggccact ttttctaccg cagtcagaat gatcagcata
    43681 tcggcatcaa tcatttctgc taaacgggcg ctggcccagt ctttatcgat aaccgcgctg
    43741 gcaccacgca gatggttacc ttcacgaata acagggatac caccaccgcc aacggtgatc
    43801 accacctggc ctgcatctac cagagcttta accgtttctt tttcaatgat atcaaccggt
    43861 tttggcgagg caacaacacg gcgatagccg cgaccagcat cttctttcag agtgtaaccc
    43921 tgttttgtca gttgctctgc ttcttgctcg gtaaagaacg aaccgatcgg cttggtcggg
    43981 ttgaggaatg ccggatcgtt agcgtcgact tcgacctggg taaccagcgt tgctacaggt
    44041 ttattaatgc cccgagaaag cagttcttct cttaaagcgt tttgtagatc gtaaccaata
    44101 taaccctggc ttaatgcaac acagacagac atcggcagca ttggcgagtg tgcttcagtt
    44161 tttgcggcag cttcaaaggc ctggttaatc atgcccacct gtggcccgtt accatgagtg
    44221 acgataactt catgtccctg ggcaattaaa tcaacaattg cctgagaagt aatttttacc
    44281 gctttcattt gcccggccag gtcatcgccc agcgcattcc cgcccagggc gagaacaatt
    44341 ttcttactca tttttcctca ctcatcattc ttgattgtgt atagatccct ggcagtggtt
    44401 agagcacggg agggacaaac ggtttgcgtc gcaggaaacg acctcgcccg gctttgcctg
    44461 taaaggtgcc gtcacagaaa atcgtttcac cacgggataa ggttctgaca atcgcgccct
    44521 gacaggtaaa accctcccat ggcgagtagt cggcgttgtc gtggagatgg cgatgctgaa
    44581 tttgttggct ctgacgtggg tcgataatca ccacgtcgcc atcggaaccg ggcgctaata
    44641 atcctttttg cggccacagg ccaaacagcc tggcgggcat cgcgctggtt aattcaacaa
    44701 aacgttccgg tgttatacgt cccgtcatca cgccactgga aaacagtaac tgcatgcggt
    44761 tttccacacc gggtaagcca tttgggcagc gactgaaatc gcctttagaa atttgcaggc
    44821 gttgagccat tgagaacgtg cagtgatcgg tcgccaccac gtcaatcgca ccatcgctga
    44881 tgccacacca cagtttgtcc tgctcgcgta cgttacgcag cggtgggcta agaatgaact
    44941 tcatgccatc ttctgtatcg tagctgcgtt cgtccaacag gagatattgt gggcaggttt
    45001 caacccagac tggctggtga ttcgcacggg caagacgcag ataatccaga cctaagccgt
    45061 tagacaggtg cacgatatag agcggggcgt taccggcaat ttgtgccagg ttaatcatgc
    45121 gggcgatggc ttccgcttcg cattccagag ggcgactcaa ggcgtgatag cgcggcgcgg
    45181 ttaaccctgc ggcgataaac tccgcccgct tgctggcgat agcagcatca ttttccgggt
    45241 gcacggtggt cagcgcgccg gattcatgca agcggcgtaa tgcctgcaaa acttcgtcat
    45301 cgttgagttt gtattgatag gttaaataga gtttaaaact gctcagtcct tcctcgacca
    45361 tcatcgggat ttcgtcgagg atggcgtgat taatgtgctg gatcacaccg tgaaagctgt
    45421 agtcgatgac cgccttatgg gcggcatagc cacgataaac ctccagttga tggcgtaacc
    45481 gacagccgtt tgggccaaat cccatatggt caataatggt tgttgtaccg ccacacgcag
    45541 ctgcgcgggt accggtaaaa aaatcatcac aactgcgcgc gatgccgaca tcaatattga
    45601 aatgcgtatg gacatccacg ccgccaggga aaacgtaaca gccagtggca tcaatttctt
    45661 catacgggag ctgcggcgaa atattgttgc ccaactggcg aacaatcccg ctttcaatca
    45721 gcaaatcctg tttggcttgt ccatctgcgt taacgacagt gccgtttttg atcaatacgc
    45781 gcatagcaaa ctccaaaaac cctccggcga accggagggg aataagtgaa gagtaattat
    45841 tcggttgcca gccagcttaa cgggattgca gcgtacatcg cggcacaggt caccaagtga
    45901 gatttccagg ttttttcgtt aggtgcatgc gcttcaggtt ctttacccgg gccaaagccg
    45961 atcaccggaa tgccgtgacg gcccatgata gatacgccgt tagttgagaa ggtccactta
    46021 tcaacaaccg gcgctttgcc aaacagccct tcgtaggcat tcaccagtgc tttaacggtg
    46081 aagtgatctt cttccacttt ccaggtcggg aagtagcatt cggttgggta aaccaggcca
    46141 gtccaggacg gacggtcgta gttgtacata gaaacaaccg cgttagcttt ctgtactgca
    46201 ggcagggcgc ggatttcgtc cagcgcgcct tcccaggttt cgccccaagt cagacggcgg
    46261 tcaatagaga cggcgcagct atctgctaca gcgcaacggc ttggggatgt gaagaagatt
    46321 tcagaaacgg tgagggtgcc tttgccgagg aattcgtcat aacccagacg ttgggagagt
    46381 tcttgtaatt cgccaagaat cggacccatt ttgaaaatgg cgttgtcacc gcgttctggc
    46441 gcagaaccgt ggcagctaac accctgaaca tcaatacgaa tttccatacg accgcgctga
    46501 ccacggtata cctggcagtc ggttggttcg gtactgacca caaattccgg gcgaatgccg
    46561 gattgttcaa taatgtactg ccagcacaga ccgtcgcagt cttcttcctg aaccgtaccg
    46621 gtaaccagca gggtatattc atcttccaga ccgaggtctt taatgatttt accggcataa
    46681 accatagatg ccatgccgcc ttcctggtcg gaagtaccgc gaccaccgat cagctcatca
    46741 gtttccatgc cttcgtacgg atcgaagtcc cagtttttga tgttgccaat gccgacggta
    46801 tcgatatgag cgtccattgc caccagacgc gggccgtggc cgatataacc gagaacgttg
    46861 cccatcgggt cgatttcaac tttatcgaag ccgacttttt ccatctcttc tttaatacga
    46921 tgtactacgc gtttctcgtc gcagctttca ctgggaatag caaccatgtc gcgcaggaag
    46981 cgagtcatgt ccgcctggta atcttttgct ttttcaagaa tcagtttgaa tggaatattc
    47041 ttagccatta tttttctcca gattttttac tccccgtttt gcagggagta gttccaatag
    47101 ggtgattaag gtgctacagc gtgtttgcct tcccagacaa cttcgcggta gtgcttcacg
    47161 tcggtgtcgc cttcagtgct gataaccagc accacggcat ctttgttcag cgccagtttt
    47221 tccatcaggc tttgacgttg cgggtgataa tgaaccgccg cgagaacgcc caaaccgaca
    47281 gcgccggatt caccggagat gatgcgcggg tcgttgccgt acggattacc cagcacgcgc
    47341 atacctaatg cggcaacgct gtcctggcag gagatgaatt gggtggcgca gttacgtagg
    47401 atttcccagc ccagcgggtt aggttcgcca caggccaggc ccgccatgat ggtggccata
    47461 tcaccgccaa cgttgacgat gtcgcctttg acgccggagc gataaataca gtcagctttg
    47521 tcaggttcaa caataatgct gtgcagattt tgcgggctaa agacgtcgac cagataaccc
    47581 agcacaccac cggccattgc tccgacaccg gcttgcagca gaacgtgcgt cggggttacg
    47641 cccatttcac gcatttgctc gacggcttca tctgccaggg ttgcgtagcc ttgcatgatc
    47701 caggttggaa ttttggtgta accttcccat gccgtgtcct gtaccacttc ccagccgtgc
    47761 tgctgcgcgt gttgcatggt caggcgaacg gtatcgtcat agttcatatc cgtgacgatg
    47821 cactcggcac cgaggttcag aatggcgtca acgcgttcct gagcagaacc tttcggcatg
    47881 taaatcaccg cattctgtcc gagttgctgc gctgcccacg ccacaccgcg cccgtggttg
    47941 ccgtcggtgg tggtcgcgaa agtcattttt tcgccgatgg catttttcag gtgctcaaat
    48001 gacagcgttt cgatatcaag atgatatttt tcacacaata attgagcgat agcgtacgca
    48061 ccgccgagca ttttgaaggc gttcagaccg aatcgttttg attcgtcttt gacaagaatt
    48121 ttcttcacac caaaaaggtt tgcgagatcg tccagcgcac aaagcggtgt tgggcgataa
    48181 ccagctattt tctggtggaa ctggcgcgcc agtttggcct ggctttgcga aaagagcggt
    48241 gatgtttcgc cgttgaaaaa tttgttatcg gcgatatcaa tcttcaatga gaaaacggac
    48301 atatcctatc cttttggcct ctggaaatag agggccgacc agctgatcgg cccgaggggt
    48361 tatttcacgc gttcttgcgc ttctttcagc agttgctcga gcagtgcacc tggtttggcg
    48421 tatttacggg acaggatcat cgcggcgatg atataaggct tccagctggc ttctttgtag
    48481 gtagcgatac ggtatttttc gaatacgcct tcagtcactt caccttcttt acaggatacg
    48541 ccgctgatat cagctggcag gcagtgcatg tacagggctt cgccatcacg ggtcagttcc
    48601 atcatctctt cagtacaatg ccagtctttg tgttgcgcgt tctgtgccag acactgtttt
    48661 tccagtgctt ttaagccttc gtgatcgttc gcacgcagca attcagtacg ctcttccatc
    48721 actttgtaag gtgcccatga cttcggataa acgatgtctg cgtctttgaa ggcttcttcc
    48781 atgctggtga cctgacggaa gctaccgccg gaggctttag cattgttttt agcgacttca
    48841 acgacatccg ggatcaggtc gtagccttcc ggatgggcca gggtgacatc cataccgaag
    48901 cgagtcatca gaccgatgat gccttgtggt acagagagcg gtttgccata gcttggggag
    48961 taggcccagg tcatggcgat ttttttacct ttcaggtttt ccaatgaacc aaagtgttca
    49021 cgtaaccatg ccaggtcagc cattgactga gtcgggtggt caatatcaca ttgcaggttc
    49081 actaaagccg gacgttgcgg cagcacaccc tgcttgtaac cgtcatcaag tgcagcgcca
    49141 acttcacgca tataggcgtt gcctgcgccc agatacatat cgtcgcgaat accaatagcg
    49201 tcggcgcaga aggagatcat attggcggtt tcacgcacgg tttcgccgtg agcgatttgt
    49261 gatttgcctt cgtcgagatc ttgctgtgca aggccgagca ggttaagcgc ggaagcataa
    49321 gagaagcggg tacgggtgga gttgtcgcgg aatacggaaa tacctaatcc actattaaag
    49381 actttggttg agatgttttc agcacgcagt gcttttaatg ctgcggcaac gtccagtact
    49441 tgtttcagtt catctggcgt ctgttcccac gttaacaaaa aatctttctc gtgaaggtga
    49501 gaggtcagcg aattgatatc cttaatcagc tcattaacag ttttcatcat aaagtcccgg
    49561 tagtggaatg acaggcaaag tggtttattt cactgtgact tgccaaagtg attggtgttt
    49621 tcacagctat acctgggact aacaataagt gtgccagaat tatccacggg gctgcaaaag
    49681 actaatttga cggataatgt gtggggttga tcacaaattg atgctagctt ccggttgttt
    49741 gtaaaatacg aaaacgtttg gcttttttta ttgcgttatt tatttgctgc taagtatcag
    49801 aaataagata aaggggcagg ctatccctcg tcagtttgat aaactcaccg ccgtaattct
    49861 catattgata tagaaatgtg atatctcttc cataatttca ttagaaagtg ataaaaatct
    49921 tatgttttta tcaaagccaa tttcgcctga gttaatatca tcatgatctg tgaaccatca
    49981 acgtcttcaa tgatcgactt ttaagtaagt ctggaaggta aagctatgga gcttgctact
    50041 acgcagtcag tattgatgca aattcaaccg acaattcagc gttttgccag aatgcttgcc
    50101 agcgttttgc agcttgaggt tgagatcgtt gatgaaaact tgtgtcgcgt cgccggaact
    50161 ggcgcgtatg ggaagtttct tggtcgccag ttgagcggca actcacgcct gctccgccac
    50221 gtcctggaaa cgaaaactga aaaagttgtg acacagtctc gcttcgatcc cctttgcgaa
    50281 ggttgcgata gtaaagaaaa ttgccgcgaa aaagcatttc tgggtacgcc tgtcatttta
    50341 caggatcgtt gtgttggggt gataagtttg attgccgtta cccacgagca acaagagcat
    50401 atcagtgata atttacgcga attttctgat tatgttcgcc atatatccac catttttgtt
    50461 tcgaaacttc tggaggatca ggggccagga gataacatca gtaaaatatt cgcgaccatg
    50521 atcgataata tggatcaggg cgtattagtt gttgatgatg aaagtcgggt tcagtttgtt
    50581 aatcagactg ccttaaaaac acttggtatt gtacaaaata atattattgg gaaacctatc
    50641 cgtttcagac cattaacatt tgagagtaat tttacccatg gacatatgca gcatattgtt
    50701 tcgtgggacg ataaaagtga attaatcatt ggccaattgc ataacattca gggccgacaa
    50761 ttatttttaa tggcgtttca ccaatcgcat accagttttt ctgtagcaaa tgcacctgat
    50821 gaaccgcata ttgaacaatt ggttggcgag tgccgtgtta tgcggcaatt aaaacgactc
    50881 attagccgta ttgcacccag cccatccagc gttatggtgg ttggtgaaag cggcacgggt
    50941 aaagaagtcg tcgcccgtgc aatccataag ttgagcggaa gacggaataa accctttatt
    51001 gctatcaact gtgccgcgat tccggagcag cttctggaga gcgaactgtt cggttatgtt
    51061 aaaggcgcat ttactggcgc ttctgccaac ggtaaaacag ggttgattca ggcggcgaat
    51121 acgggcacgc tgtttctcga tgaaataggt gatatgccat taatgttgca ggctaaatta
    51181 ctgcgcgcta ttgaggcacg tgaaattctg ccgattggtg ccagtagccc aatacaagtc
    51241 gacattcgca tcatttctgc aactaaccag aatttggccc agttcattgc cgaaggtaaa
    51301 ttccgcgaag atctcttcta ccgacttaat gttatcccga taactctgcc accgctgcgt
    51361 gaacgtcagg aagatattga actactggtg cattactttt tacatctgca tacccgtcgt
    51421 ctgggatcgg tttatcctgg cattgctccc gatgtcgtcg aaatattgcg taagcatcgt
    51481 tggcccggaa acctgcgcga gttaagcaat ttgatggaat atctggttaa cgttgttcct
    51541 tcaggtgaag ttatcgacag cacgctattg ccgccaaatc tgctgaataa tggcacaacg
    51601 gagcaaagtg atgtaacaga ggtcactgag gcacacctgt cactcgatga tgcgggcggc
    51661 acggcgctgg aggagatgga aaagcaaatg atccgcgagg cgctttcacg tcataacagc
    51721 aagaagcaag ttgctgatga actgggcatc ggcattgcta cgctctatcg caagattaag
    51781 aaatatgagt tgttaaacac ataacatcag tgaatccggc ctgaattcag gccggatatc
    51841 cttttacttc gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca
    51901 gcgacaaaga tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg
    51961 tggtttcgcc agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg
    52021 cactgcgccg gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc
    52081 accttccagc gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc
    52141 tattgctgtg ccgtcgacca gcaccgtaca ggcaccacat tcacccacgc agcacccttg
    52201 tttgacactt agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc
    52261 atgaagctga aaaggcatcc cgttaatggt gcattcgatg gtaattgttt cgctgtgatt
    52321 cattgcaatt ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc
    52381 tggatgagat gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct
    52441 tgcaggacag attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca
    52501 gtctgttcgg catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccagacgt
    52561 aattcgctga aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata
    52621 tccattgcgt cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgg
    52681 ggcggaaaat gaaaggcgac aaggatttcg tcatgctcaa gagacacttt gcctggccca
    52741 gtgtgaaagc cattaatcgg gacgaaacga acaccgcgtg gggagtggat ctccagcttt
    52801 gcgtcataaa ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt
    52861 ccaccgtagg tagcgacgtt acggatctgc ggtccggcaa tggacgaggc cgcagcacat
    52921 aacgccggga gatgacgttg agttatagaa tcttctatta gctgggtaaa tgtcgttgca
    52981 gagccgatac gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta
    53041 tgaatatcaa caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca
    53101 gtgccaccgg cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca
    53161 agggttgctg cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc
    53221 tgccagatga aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac
    53281 accggtagcc atcttcaccg cgttacgaat agctgcggca acaggaatta ttggtggctc
    53341 acccagtgac ttatgtccgt atgcggattg cggttcattg atttcgacga acgcgctttc
    53401 cagttgtggc agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac
    53461 cacgccgctt ttcgcatcga tgatcatctc ttcaaatagc gcccagccaa tgcccattcc
    53521 cattccgccg tgtacctgac cttctgccag cagcggattt agaatatgcc ccgaatcatg
    53581 aacgttgagg atgcggttga tggtgacttt gcacagtgca atatcgaccg tcagatcaac
    53641 aaaggtacag ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag
    53701 ctgcccgccg cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa
    53761 cggctcttcc ggtcgttcaa ccagcacgat atggcctttt atcagggtca gattcatcgc
    53821 tgactgatgt agcatgactg cggcgtgagc gatgattttc tcttttaata acagtgccgc
    53881 actgcgcagc gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa
    53941 cggcgtaacg tcggtatctt gtgttgaaat aacgcgaacg tcgctaaccg gaacccccac
    54001 ggtttctgcc accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc
    54061 gctttgcacg ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac
    54121 gccgacaggc caggtattag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa
    54181 attgccttgc tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc
    54241 tttttcaaga cactccggca accctgcgct gtaaatacgt ttgcccgtga gcggattagc
    54301 atctccttcg cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt
    54361 cgcggcgtca tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg
    54421 catcgcacca gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca
    54481 acgaggataa aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga
    54541 tgcataagcg ccggtgttag acagaacatc cagactataa cctttcaatg ttccgtcgcg
    54601 gctcacgccc atttgcccgt caatagtaaa agcgtggcgg gtacgggttg cgaggaaaca
    54661 ctcttcacgg ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc
    54721 cgccattggc tcttccagta catcctgttt attaccaaaa ccgccaccga caaatggttt
    54781 gatgactcgt acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat
    54841 gtgcgggatc tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag
    54901 cgatgttacg ctttccatgt gacaatgttg aataacgggg gtctgatagt gcccctgtac
    54961 ctggtagtcg gcggcatcga ttgtttgttg gacattgccc gtcgacatcg tgctttgttt
    55021 cagtaaattg ccgccgttat ggattggtgc agcgtcctct gccagcgccg cttctggcgt
    55081 ggtgataacg ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt
    55141 gagttcatcg cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt
    55201 tagcagtgcg cgatcggcgg tatcgcgctt gttttcgtca agtgtccatg catgcccagc
    55261 tgtagcgaat gggatatcag gcacatcttc ccaggtaaaa atcgccagta cgcctggtaa
    55321 acttctggct tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac
    55381 atatttcgca taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt
    55441 gaccttagca atggcatcaa cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc
    55501 ttccatagaa atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc
    55561 aggcaagatt tacgccagaa acgacgataa gcgttagggg aggaaggaag tgtgatgacg
    55621 ttaaaaaaac acagatatga tacgaaacag gactctgaat aggggcagtg atacgtatca
    55681 atatgagtgg cgataaaatc aatgtgataa attgggatta ttacgcatca ggatgggaaa
    55741 tagggtaaaa aagtcaggaa attcgatacc tctacagcga aaattttgct gatgatactt
    55801 ccctttgcca aatgaaatta acctctttct ctattaccgc gtttttccag aagcaagaga
    55861 ttgggtatca agaggctggc tgctatgata tggcgtcttg ttttttaagc gaggaaagat
    55921 tttgagtgcg ggacgcctga ataaaaaatc tctgggtatc gtgatgttgt tatcggttgg
    55981 actgcttttg gcgggctgtt cgggtagcaa atcatccgat acaggaacgt attccggctc
    56041 cgtttacacc gtgaaacggg gggatacgct atatcgtatt tcgcgcacca cgggaaccag
    56101 cgtaaaagag ctggcgcgac tgaacggcat ttccccccct tacaccattg aagttggtca
    56161 gaaactaaaa ctgggtgggg cgaaaagtag cagtagtaca cgtaaatcaa ccgccaaatc
    56221 aacgaccaaa accgcatcgg ttacaccgtc atcagcggta ccgaaatctt cctggccgcc
    56281 agtagggcaa cgttgttggt tatggccaac gacagggaaa gttatcatgc cgtattcgac
    56341 agcagatggc ggcaataaag ggattgatat ctctgctcca cggggtacac ctatttacgc
    56401 cgcgggtgca ggaaaggtgg tgtatgtggg caaccagctg cgtggctacg gtaatctcat
    56461 catgattaaa cacagtgaag attacattac ggcttacgcc cataatgaca cgatgctggt
    56521 aaataatggg caaagcgtga aggctgggca aaaaatcgcc accatgggga gcacggatgc
    56581 ggcatctgtt cgcctgcatt tccagattcg ttaccgtgca acggcaattg atccgctacg
    56641 ttacttgccg ccgcagggca gcaagccaaa atgctgatgg cgaattaatc agcagtcagc
    56701 agcgcggcac ttgctaagga gagcgtaagg tttataatgc cttacgcatc tcaaagcggg
    56761 cgtagttcaa tggtagaacg agagcttccc aagctctata cgagggttcg attcccttcg
    56821 cccgctccaa tttatcttca cctcatcaac ctgccatttt tgttgtatct gattttctat
    56881 attatcttaa ttcaatcggt tcagactttt cgcaatcgca gtaacacttc cccatttttt
    56941 tgtaaataaa aagcgtttac gcaatgcaat ccttttataa caacccaggt aaaatacctt
    57001 attttcaagt tgttaaataa gtggttatct acgcttttaa caaacgattg atggaatatt
    57061 ttatgaaagg gaaatctgca ttaactcttc tgctcgctgg tatattttcg tgcggcacat
    57121 gccaggcgac aggggcagaa gtgacgagtg aatctgtatt taacattctt aattccacgg
    57181 gggcagcaac agataaaagt tatttatcgt taaatccaga taagtatcct aattatcgtc
    57241 ttctgattca ctcagccaaa ttacaaaatg aaataaagag ccactatacc aaagacgaaa
    57301 ttcaaggatt gctaacatta acagaaaaca cacgaaagtt aacgttgacg gagaaaccct
    57361 ggggaacgtt tattcttgct tctactttcg aagatgacaa aacagcggca gaaacccact
    57421 atgatgctgt ctggctgagg gatagtttat ggggctatat ggcattagta tcagaccagg
    57481 gaaatagtgt agccgcaaaa aaggttctgc taaccctgtg ggattatatg tctacgcccg
    57541 accagattaa gcggatgcag gacatcatta gcaacccaaa acgactggat ggtattcccg
    57601 ggcaaatgaa tgctgtgcat atccgatttg acagcaactc ccccgtgatg gcagatgtgc
    57661 aggaagaagg caagccgcag ctgtggaacc ataaacaaaa tgatgcgtta ggtctttatc
    57721 ttgatctcct tatacaagca atcaatactg gtactattaa cgctgaagat tggcaaaaag
    57781 gtgaccgact aaaatcggtt gcgctgctga ttgcttatct ggataaagcc aatttctatg
    57841 ttatggaaga ttccggagcg tgggaggaag atgcccgatt aaacacatct tccgttgcac
    57901 tggtgacctc tggactggaa cgtttgtcaa acctactctc gaagaaagac tctgtttttg
    57961 tctctgattt actacgcgaa gctaaagtca atgaactgga tgaaactctt tcaacaacga
    58021 ggttgaacca cctcatagat aaaggttatg aaagaataac gttacagctt gatttagggg
    58081 gagaatcccc aggttatctg gaaaaagata aacattacag agaagccgat gcggctttac
    58141 ttaatgttat ttatcctgca aatctttcaa agataaacac cagaagaaaa gagcaagttc
    58201 taaaaatagt taaaaaacta gcaggtccgt atggtataaa aagatacgag aaggataatt
    58261 atcagtcggc aaatttttgg ttcaatgata ttaaaaccga taccgatcaa aattcgcatg
    58321 caaaaagaga aaagagcttc attccatcaa cagaagcaga atggtttttt gactcatggt
    58381 atgcaaaatc agcagcaatt gtttataaag aatcaagaaa agaagagtat ttgaatgact
    58441 cagtacaatt tatgaataga tcattggccc aaataacagg cgagaatatg attggtgcaa
    58501 atggcaggag cgtaccagaa atggcacttc ccgaaagcta taactatatc cacaagtcgg
    58561 gaactttgca tgaagcgcca agccctatca ttccgctaaa ttggtcgaaa gcttcaatga
    58621 ctctcatgtt gaaagagatg tccaacctta tcaatgatga gggaattaaa tagttttcat
    58681 aatgaggcgg tcctggatta aaaggaccgc tcaaagagaa atcgaaaggg gaggtaaaag
    58741 tgatcaataa attactgtta gcttacctga taggtctggt tgtgacatcg actttaatat
    58801 ttatcttctc ggaagaaaaa gtaacatatc ggttgtttgc tgcaataatt acaggtttaa
    58861 cctggccgct tagtctcatt ccttccatta taagtctcat gattcgtaaa tccgactaac
    58921 aagaaaatta tatgatcgat taagaaaaat agaatcgaac aagagaataa aatatatgcg
    58981 gggagtaaca aaacactcgc agtgtgttaa tgaagaacta acaaatatga tttgtattga
    59041 aaatagttac caaacaagtg ttataagaaa tacgaggtgt gtttaagtaa aa
//
LOCUS       DAZFKF010000021        58626 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFKF010000021 DAZFKF010000000
VERSION     DAZFKF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.021"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            86..649
                     /gene="srlA"
                     /locus_tag="ACHKA1_001588"
                     /gene_synonym="gutA"
     CDS             86..649
                     /gene="srlA"
                     /locus_tag="ACHKA1_001588"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO5679262.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            646..1605
                     /gene="srlE"
                     /locus_tag="ACHKA1_001589"
                     /gene_synonym="gutE"
     CDS             646..1605
                     /gene="srlE"
                     /locus_tag="ACHKA1_001589"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO5679263.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLVQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1616..1987
                     /gene="srlB"
                     /locus_tag="ACHKA1_001590"
                     /gene_synonym="gutB"
     CDS             1616..1987
                     /gene="srlB"
                     /locus_tag="ACHKA1_001590"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO5679264.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1991..2770
                     /gene="srlD"
                     /locus_tag="ACHKA1_001591"
     CDS             1991..2770
                     /gene="srlD"
                     /locus_tag="ACHKA1_001591"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO5679265.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2876..3235
                     /gene="gutM"
                     /locus_tag="ACHKA1_001592"
     CDS             2876..3235
                     /gene="gutM"
                     /locus_tag="ACHKA1_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO5679266.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3302..4075
                     /gene="srlR"
                     /locus_tag="ACHKA1_001593"
                     /gene_synonym="gutR"
     CDS             3302..4075
                     /gene="srlR"
                     /locus_tag="ACHKA1_001593"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO5679267.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            4068..5033
                     /gene="gutQ"
                     /locus_tag="ACHKA1_001594"
     CDS             4068..5033
                     /gene="gutQ"
                     /locus_tag="ACHKA1_001594"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO5679268.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLVGAINLQDFYQAGII"
     gene            complement(5030..6544)
                     /gene="norR"
                     /locus_tag="ACHKA1_001595"
     CDS             complement(5030..6544)
                     /gene="norR"
                     /locus_tag="ACHKA1_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO5679269.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAARFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            6731..8170
                     /gene="norV"
                     /locus_tag="ACHKA1_001596"
     CDS             6731..8170
                     /gene="norV"
                     /locus_tag="ACHKA1_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO5679270.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            8167..9300
                     /gene="norW"
                     /locus_tag="ACHKA1_001597"
     CDS             8167..9300
                     /gene="norW"
                     /locus_tag="ACHKA1_001597"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO5679271.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVMNIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(9624..11876)
                     /gene="hypF"
                     /locus_tag="ACHKA1_001598"
     CDS             complement(9624..11876)
                     /gene="hypF"
                     /locus_tag="ACHKA1_001598"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO5679272.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLEGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRVFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLTTFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(12029..12556)
                     /gene="hydN"
                     /locus_tag="ACHKA1_001599"
     CDS             complement(12029..12556)
                     /gene="hydN"
                     /locus_tag="ACHKA1_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO5679273.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(12706..13716)
                     /gene="ascG"
                     /locus_tag="ACHKA1_001600"
     CDS             complement(12706..13716)
                     /gene="ascG"
                     /locus_tag="ACHKA1_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO5679274.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVMGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            13976..15433
                     /gene="ascF"
                     /locus_tag="ACHKA1_001601"
     CDS             13976..15433
                     /gene="ascF"
                     /locus_tag="ACHKA1_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO5679275.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEIVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAISGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            15442..16866
                     /gene="ascB"
                     /locus_tag="ACHKA1_001602"
     CDS             15442..16866
                     /gene="ascB"
                     /locus_tag="ACHKA1_001602"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO5679276.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(17025..17495)
                     /gene="hycI"
                     /locus_tag="ACHKA1_001603"
     CDS             complement(17025..17495)
                     /gene="hycI"
                     /locus_tag="ACHKA1_001603"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO5679277.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(17488..17898)
                     /gene="hycH"
                     /locus_tag="ACHKA1_001604"
     CDS             complement(17488..17898)
                     /gene="hycH"
                     /locus_tag="ACHKA1_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO5679278.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(17895..18662)
                     /gene="hycG"
                     /locus_tag="ACHKA1_001605"
     CDS             complement(17895..18662)
                     /gene="hycG"
                     /locus_tag="ACHKA1_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO5679279.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGNMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(18662..19204)
                     /gene="hycF"
                     /locus_tag="ACHKA1_001606"
     CDS             complement(18662..19204)
                     /gene="hycF"
                     /locus_tag="ACHKA1_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO5679280.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(19214..20923)
                     /gene="hycE"
                     /locus_tag="ACHKA1_001607"
     CDS             complement(19214..20923)
                     /gene="hycE"
                     /locus_tag="ACHKA1_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO5679281.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(20941..21864)
                     /gene="hycD"
                     /locus_tag="ACHKA1_001608"
     CDS             complement(20941..21864)
                     /gene="hycD"
                     /locus_tag="ACHKA1_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO5679282.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(21867..23693)
                     /gene="hycC"
                     /locus_tag="ACHKA1_001609"
     CDS             complement(21867..23693)
                     /gene="hycC"
                     /locus_tag="ACHKA1_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO5679283.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(23690..24301)
                     /gene="hycB"
                     /locus_tag="ACHKA1_001610"
     CDS             complement(23690..24301)
                     /gene="hycB"
                     /locus_tag="ACHKA1_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO5679284.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(24426..24887)
                     /gene="hycA"
                     /locus_tag="ACHKA1_001611"
     CDS             complement(24426..24887)
                     /gene="hycA"
                     /locus_tag="ACHKA1_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO5679285.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            25099..25449
                     /gene="hypA"
                     /locus_tag="ACHKA1_001612"
     CDS             25099..25449
                     /gene="hypA"
                     /locus_tag="ACHKA1_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO5679286.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            25453..26325
                     /gene="hypB"
                     /locus_tag="ACHKA1_001613"
     CDS             25453..26325
                     /gene="hypB"
                     /locus_tag="ACHKA1_001613"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO5679287.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     TPEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            26316..26588
                     /gene="hypC"
                     /locus_tag="ACHKA1_001614"
     CDS             26316..26588
                     /gene="hypC"
                     /locus_tag="ACHKA1_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO5679288.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            26588..27709
                     /gene="hypD"
                     /locus_tag="ACHKA1_001615"
     CDS             26588..27709
                     /gene="hypD"
                     /locus_tag="ACHKA1_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO5679289.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     QRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            27706..28716
                     /gene="hypE"
                     /locus_tag="ACHKA1_001616"
     CDS             27706..28716
                     /gene="hypE"
                     /locus_tag="ACHKA1_001616"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO5679290.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLIAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLASLYGVKRTLDLPHAEPLPR
                     IC"
     gene            28790..30868
                     /gene="flhA"
                     /locus_tag="ACHKA1_001617"
     CDS             28790..30868
                     /gene="flhA"
                     /locus_tag="ACHKA1_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO5679291.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            30913..31341
                     /locus_tag="ACHKA1_001618"
     CDS             30913..31341
                     /locus_tag="ACHKA1_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFO5679292.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(31380..31724)
                     /gene="ygbA"
                     /locus_tag="ACHKA1_001619"
     CDS             complement(31380..31724)
                     /gene="ygbA"
                     /locus_tag="ACHKA1_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO5679293.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            complement(31801..31935)
                     /locus_tag="ACHKA1_001620"
                     /pseudo
     CDS             complement(31801..31935)
                     /locus_tag="ACHKA1_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32011..34572
                     /gene="mutS"
                     /locus_tag="ACHKA1_001621"
     CDS             32011..34572
                     /gene="mutS"
                     /locus_tag="ACHKA1_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO5679294.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIAPGYNEELDEWRALADGATDYLERL
                     EIRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            34678..35334
                     /gene="pphB"
                     /locus_tag="ACHKA1_001622"
     CDS             34678..35334
                     /gene="pphB"
                     /locus_tag="ACHKA1_001622"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO5679295.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FELNDMEQKEAIGLLLKFHQLPHIIEITNDIIKYVIAHADYPGNEYLFGKQIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMLDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(35375..35611)
                     /locus_tag="ACHKA1_001623"
     CDS             complement(35375..35611)
                     /locus_tag="ACHKA1_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO5679296.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(35622..37049)
                     /locus_tag="ACHKA1_001624"
     CDS             complement(35622..37049)
                     /locus_tag="ACHKA1_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO5679297.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESSYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(37049..37642)
                     /locus_tag="ACHKA1_001625"
     CDS             complement(37049..37642)
                     /locus_tag="ACHKA1_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO5679298.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            37789..38196
                     /locus_tag="ACHKA1_001626"
     CDS             37789..38196
                     /locus_tag="ACHKA1_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO5679299.1"
                     /translation="MALRNKAFHQLRQLFQQHTSRWQHELPGLTKPQYAVMRAIADKP
                     GIEQVALMEAAVSTKATLAEMLARMENRGLVRRDHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSAEEQELFVQLVRKMMNT"
     gene            complement(38316..39308)
                     /gene="rpoS"
                     /locus_tag="ACHKA1_001627"
     CDS             complement(38316..39308)
                     /gene="rpoS"
                     /locus_tag="ACHKA1_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO5679300.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(39371..40459)
                     /gene="nlpD"
                     /locus_tag="ACHKA1_001628"
     CDS             complement(39371..40459)
                     /gene="nlpD"
                     /locus_tag="ACHKA1_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO5679301.1"
                     /translation="MVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKMGT
                     TSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSYS
                     GSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGGN
                     AITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVTAP
                     VTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAII
                     ATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMGS
                     TGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(40650..41276)
                     /gene="pcm"
                     /locus_tag="ACHKA1_001629"
     CDS             complement(40650..41276)
                     /gene="pcm"
                     /locus_tag="ACHKA1_001629"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO5679302.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(41270..42031)
                     /gene="surE"
                     /locus_tag="ACHKA1_001630"
     CDS             complement(41270..42031)
                     /gene="surE"
                     /locus_tag="ACHKA1_001630"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO5679303.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPAEQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(42012..43061)
                     /gene="truD"
                     /locus_tag="ACHKA1_001631"
     CDS             complement(42012..43061)
                     /gene="truD"
                     /locus_tag="ACHKA1_001631"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO5679304.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELVELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(43058..43537)
                     /gene="ispF"
                     /locus_tag="ACHKA1_001632"
     CDS             complement(43058..43537)
                     /gene="ispF"
                     /locus_tag="ACHKA1_001632"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO5679305.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(43537..44247)
                     /gene="ispD"
                     /locus_tag="ACHKA1_001633"
     CDS             complement(43537..44247)
                     /gene="ispD"
                     /locus_tag="ACHKA1_001633"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO5679306.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(44266..44577)
                     /gene="ftsB"
                     /locus_tag="ACHKA1_001634"
     CDS             complement(44266..44577)
                     /gene="ftsB"
                     /locus_tag="ACHKA1_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO5679307.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(44771..45094)
                     /gene="ygbE"
                     /locus_tag="ACHKA1_001635"
     CDS             complement(44771..45094)
                     /gene="ygbE"
                     /locus_tag="ACHKA1_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO5679308.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            complement(45144..45749)
                     /gene="cysC"
                     /locus_tag="ACHKA1_001636"
     CDS             complement(45144..45749)
                     /gene="cysC"
                     /locus_tag="ACHKA1_001636"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO5679309.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(45749..47176)
                     /gene="cysN"
                     /locus_tag="ACHKA1_001637"
     CDS             complement(45749..47176)
                     /gene="cysN"
                     /locus_tag="ACHKA1_001637"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO5679310.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(47178..48086)
                     /gene="cysD"
                     /locus_tag="ACHKA1_001638"
     CDS             complement(47178..48086)
                     /gene="cysD"
                     /locus_tag="ACHKA1_001638"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO5679311.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            48338..49375
                     /gene="iap"
                     /locus_tag="ACHKA1_001639"
     CDS             48338..49375
                     /gene="iap"
                     /locus_tag="ACHKA1_001639"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO5679312.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   49457..49973
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=49457..49485
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(50070..50363)
                     /gene="cas2e"
                     /locus_tag="ACHKA1_001640"
     CDS             complement(50070..50363)
                     /gene="cas2e"
                     /locus_tag="ACHKA1_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO5679313.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(50360..51283)
                     /gene="cas1e"
                     /locus_tag="ACHKA1_001641"
     CDS             complement(50360..51283)
                     /gene="cas1e"
                     /locus_tag="ACHKA1_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO5679314.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGYIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(51280..51930)
                     /gene="cas6e"
                     /locus_tag="ACHKA1_001642"
     CDS             complement(51280..51930)
                     /gene="cas6e"
                     /locus_tag="ACHKA1_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO5679315.1"
                     /translation="MYLSRITLHTAQLLPAQLLHLVDRGEYVMHQWLWDLFPSGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFAPELRTGQSLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGRDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEV"
     gene            complement(51912..52658)
                     /gene="cas5e"
                     /locus_tag="ACHKA1_001643"
     CDS             complement(51912..52658)
                     /gene="cas5e"
                     /locus_tag="ACHKA1_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO5679316.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTIQMPKEVRKARYFSRREELSDPD
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQGGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDHFHDLKVSLPKLQDECWWEGEHDGLVVSKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(52669..53724)
                     /gene="cas7e"
                     /locus_tag="ACHKA1_001644"
     CDS             complement(52669..53724)
                     /gene="cas7e"
                     /locus_tag="ACHKA1_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO5679317.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(53739..54275)
                     /gene="casB"
                     /locus_tag="ACHKA1_001645"
                     /gene_synonym="cse2"
     CDS             complement(53739..54275)
                     /gene="casB"
                     /locus_tag="ACHKA1_001645"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO5679318.1"
                     /translation="MSIVKEEHKATLRKWHEELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSEGRFTRL
                     SAVKTPDDLLRQLRRAVKLLNGSVNLISLAEDIFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQN"
     gene            complement(54272..55834)
                     /gene="casA"
                     /locus_tag="ACHKA1_001646"
                     /gene_synonym="cse1"
     CDS             complement(54272..55834)
                     /gene="casA"
                     /locus_tag="ACHKA1_001646"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO5679319.1"
                     /translation="MNSFSLLTTPWLPVRYKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFEALTGDKVQVASLLPDVPGAQTTKFNKDHFIKRGVTEYLCPHCLALALFSLQLNA
                     PAGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWLNVMPQDEADLPLPQKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDPDELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQNA
                     KAAREKKQEAAE"
     gene            complement(55931..>58626)
                     /gene="cas3"
                     /locus_tag="ACHKA1_001647"
     CDS             complement(55931..>58626)
                     /gene="cas3"
                     /locus_tag="ACHKA1_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFO5679320.1"
                     /translation="KYPLSLLKDKNIVTFFDFWGKTRRGEKEGGDDYHLLCWHSLDVA
                     AMGYLMVKRNCFGLADYFYQLGISDKEQAAQFFAWSLCWHDIGKFAHSFQQLYLPPEL
                     KIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGH
                     HGRPPDRMDELDNFLPEDKAAARDFLLAIKALFPRIEIPAFWDDDEGIELIKQLSWYI
                     SATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFTGINT
                     LFPFIENPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAAGKAQGL
                     FFGLPTMATANTMYDRLVKTWLSFYSPESRPSLVLAHSARTLMDRFNESLWSGDLIGS
                     EEPDEQAFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKIL
                     LADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVTAFARGTEGQQE
                     APLLEKDDYPWLTHVTQTDVNSHRVATRKDVERSVSVGWLHSEQECIARIESSVSQGK
                     CIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIEMETLARFGKEDG
                     SQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGK
                     DERSPPELLILAPVWDDSPGDEWFGRAMRNSAFVYPDHGRIWLTQRVLRGQGAIQMPH
                     AARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRTMAKKFVLNFRPGYAASINDYLP
                     EKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEG
                     DAFRQWCIEQRQEPEMANVILVTDDESCGYSAMEGLTGKVG"
BASE COUNT        13844 a        16077 c        15212 g        13493 t
ORIGIN      
        1 tcaaaataac acttttaaat ctttcaatct gattagatta ggttgccgtt tggtaataaa
       61 acaataaatc ctgaaggaga gaacaatgat agaaaccatt actcatggtg cagagtggtt
      121 tatcgggctg ttccaaaagg gcggagaggt gtttaccggg atggtgaccg gcattcttcc
      181 gctgttgatt agcctgctgg ttatcatgaa cgcactgatt aattttatcg gtcagcatcg
      241 aattgaacgt tttgctcaac gttgcgccgg taaccctgtt tcccgttacc tgctgctacc
      301 gtgcattggc acgtttgtct tttgcaatcc gatgacccta agtctgggtc gctttatgcc
      361 ggaaaagtac aaacccagct actacgcggc cgcctcttat agctgccact caatgaatgg
      421 cctcttcccc catatcaacc ctggcgaact gtttgtttat cttggcattg ccagcggtct
      481 gacaacgctg aacctgccac ttggcccact ggcggtgagc tatctgctgg ttggtctggt
      541 caccaacttc ttccgcggct gggtgaccga tctgaccacc gccatttttg agaaaaagat
      601 gggtattcaa cttgaacaaa aagttcacct ggcaggagca acatcatgac gcgtattcgg
      661 atcgaaaaag gaacgggtgg ctggggcggc ccgcttgagc tggaagccac gccgggcaaa
      721 aaaatcgtct atatcaccgc cggtacccgg cctgcgattg ttgacaaact ggtacagctt
      781 actggctggc aggctattga cggatttaaa gaaggtgaac ccgcggaggc ggaaattggt
      841 gtcgcggtaa tcgactgtgg cggcacatta cgctgcggca tttatccgaa acggcgtatt
      901 cccaccatta atatccactc gacgggcaag tccggtccac tggcgcagta cattgtggaa
      961 gatatttatg tctctggcgt aaaagaagaa aacatcactg tagtgggtga tgcgacacca
     1021 caaccctctt ccgtgggccg tgactatgac accagcaaga aaatcaccga acaaagcgat
     1081 ggtttactgg cgaaggtggg aatgggtatg ggttctgccg ttgccgtgtt gtttcaatct
     1141 ggtcgtgaca ccatcgacac tgtattaaaa accattctgc cgtttatggc attcgtctcg
     1201 gcgctcattg gcatcattat ggcttctggc cttggtgact ggattgccca cggtcttgct
     1261 ccgctggcga gccatccact gggtctggtc atgctggcgc tcatctgctc cttcccgctg
     1321 ctttcacctt tcctcggccc aggcgcagtt atcgcacagg ttatcggcgt attgattggc
     1381 gtgcagattg gtctcggcaa tattccgccg catctggctt tacctgcgct gtttgccatc
     1441 aacgcgcagg cggcctgcga cttcatcccg gtcggtttgt cgctggcgga agcccgtcag
     1501 gacacggttc gcgtcggtgt cccttctgtt ctggtgagcc gctttttaac cggcgcaccg
     1561 actgtactga tcgcctggtt tgtctccggt tttatctatc aatagaggct gaaacatgac
     1621 cgttatttat cagaccacca tcacccgtat cggcgcgagt gccactgacg ccctcagcga
     1681 ccagatgctc atcacctttc gtgaaggcgc gcctgcggat ctcgaagagt attgcttcat
     1741 tcattgccac ggcgagttga aaggtgcact ccatcccggt ttgcaatttt cgctcgggca
     1801 gcatcgctat ccggtgaccg ccgttggcag cgtggcggaa gacaaccttc gcgaactggg
     1861 tcatgtcacc ctgcgcttcg atggtttaaa cgaagcggaa tttccgggca ctgtccatgt
     1921 ggcaggccct gtccccgacg atatcgcgcc gggatcggtt ttgaagtttg aatctgttaa
     1981 ggagtaaaaa atgaatcagg ttgccgttgt catcggtggt gggcaaacct taggcgcgtt
     2041 cctgtgccac ggtctggctg ccgaggggta tcgcgtcgcg gttgtcgata ttcagagcga
     2101 caaagccgca aatgtggcac aagaaattaa cgccgaatat ggtgaaggta tggcgtacgg
     2161 ttttggtgct gacgccacca gtgagcaaag cgttctggcg ctctctcgtg gggtagatga
     2221 aatctttggt cgcgtggatt tgctggtcta cagcgccgga attgcaaaag cagcctttat
     2281 cagcgacttc cagctcggtg attttgaccg ttcgctacag gtgaatctgg tgggttattt
     2341 cctgtgtgcg cgtgaatttt cgcgtttgat gatccgcgat gggattcagg gacgcattat
     2401 tcagatcaac tcgaaatccg gcaaagtggg cagcaaacac aactctggct acagcgcagc
     2461 gaaatttggt ggcgtagggc tgactcaatc actggcgctg gatctggcgg agtacggcat
     2521 tacggtgcat tcactgatgc tcggtaacct gctgaaatcg ccgatgttcc agtcgctgtt
     2581 gccacaatac gcgaccaagc tgggtatcaa accggatcaa gtcgagcagt attacatcga
     2641 taaagtaccg ctcaaacgcg gctgcgatta tcaggatgtg ctgaatatgc tgctgttcta
     2701 cgccagtcct aaggcatcgt actgcactgg tcagtcgatc aatgtcaccg gcggtcaggt
     2761 gatgttctga tcaacagcgg agatccgtta aggatctccg tgggactaat agaatgcctg
     2821 atgcgctacg ctcatcaggc atacaggact tccgccacta cattaaggaa aagttatggt
     2881 atccgcactc atcactgtcg ccgttatcgc ctggtgtgcg caactggcct taggcggctg
     2941 gcaaatttct cgttttaacc gtgccttcga cacactatgc cagcaagggc gggttggcgt
     3001 gggccgttcc agcgggcgct ttaaaccgcg ggtcgtggtc gccatcgccc tggacgatca
     3061 gcagcgcatc gccgacacct tgtttatgaa aggactgacc gtcttcgccc gaccgcaaaa
     3121 aattcccgca attaccggca tgcatgcggg tgatttacag cccgatgtga tctttcccca
     3181 tgatccacta tcacagaatg ctctatcatt ggcgcttaaa ctgaaacgtg gataatttcg
     3241 ttgtgaatgt tacttgcttg cgaagttatc attttgaaac ctaaatcagg taatcacgcc
     3301 catgaaacct cgtcagcgtc aggccgccat tctggagtat ctgcaaaagc agggtaaatg
     3361 ctcggttgaa gaattggcgc aatactttga caccacaggc acaaccattc gcaaagatct
     3421 ggtcattctg gaacatgccg gaaccgtcat tcgtacttat ggcggagtgg tgttgaataa
     3481 agaggaatcc gatccgccta tcgatcataa aacactcatc aacacccaca agaaagagct
     3541 gatagcagaa gctgccgttg gttttattca tgatggcgat tcgatcattc ttgatgctgg
     3601 cagcaccgtt ttgcagatgg tgcctctgct ctcgcgcttt aataacatca cggtgatgac
     3661 caacagcctg catatcgtca atgcgctatc cgaactggat aacgaacaaa ctatcctgat
     3721 gccaggcgga acgtttcgta aaaaatcggc ctcatttcac gggcagctgg cagagaatgc
     3781 cttcgagcat ttcaccttcg acaaattatt catgggtact gacggcatcg atctcaatgc
     3841 gggcgtaacc accttcaacg aggtctatac cgtcagtaag gcaatgtgca atgccgcgcg
     3901 cgaagtgatt ttgatggcgg actcatcaaa gtttggccgt aaaagcccca acgtagtttg
     3961 cagtcttgaa agcgtcgata agctgattac cgacgcagat atcgatccgg cgtttcgtca
     4021 ggcgctggaa gagaaaggga tcgatgtgat cataaccgga gagagcaatg agtgaagcac
     4081 tactgaacgc gggacgtcag acgttaatgc tggaattgca ggaagcaagc cgtttaccgg
     4141 aacgtctggg cgatgatttt gttcgcgccg ccaatatcat cctgcactgc gaaggcaaag
     4201 tggtggtttc gggaattggc aaatcgggcc acattggtaa gaaaatcgcc gcaacgcttg
     4261 ccagtaccgg cacaccggct ttttttgtcc atccggcaga agcgctgcac ggcgatctgg
     4321 ggatgatcga aagccgcgat gtgatgctgt ttatctctta ctccggtggg gcgaaggaac
     4381 tggatctgat tattccgcgt ctggaagaca aatctatcgc gctgctggcg atgaccggca
     4441 aaccgacgtc accgctgggc ctggcggcga aagcggtgct ggatatctcc gtagaacgcg
     4501 aagcctgccc gatgcacctt gcgccgacct ccagcaccgt caataccctg atgatgggtg
     4561 acgcgctggc gatggcagtc atgcaggcgc gcggatttaa tgaagaagat tttgcccgct
     4621 cccaccctgc tggagcactg ggcgctcgct tgctgaataa agtgcatcat ctgatgcgcc
     4681 gtgacgatgc catcccacag gtggcgttaa ccgccagcgt gatggatgcg atgctggaac
     4741 tcagccgcac cggtctgggg ctggtggcgg tatgtgacgc tcaacaacag gtacaaggcg
     4801 tctttaccga cggcgattta cgtcgctggc tggttggcgg cggcgcactc accacgccag
     4861 tcaatgaagc gatgacgaca ggcggcacca cattacaggc acaaagtcgc gccatcgacg
     4921 ccaaagagat cctgatgaag cgcaaaatca ctgccgcacc ggtggtggat gaaaacggca
     4981 aactcgtcgg cgcaattaac ctgcaggatt tctatcaggc cgggattatt taatccttca
     5041 atcccagacg tttcgccagc cgatgcaggt tggcgacgtc ggtttccagc attcgcgcgc
     5101 aggctgccca gttgtgatga ttttgtgcca gtgcctgacg aatagtttca cgctggaacg
     5161 cttctgtcgc ttcacgcagg ttttgcttaa caacgggcac cgccgccgct tctggcggcg
     5221 gtaacgtcac ctcaggaaaa gcaaaatgtt gcgcctcaag aatcacttca tcgccgttgc
     5281 gggtggctct cgccagcact accgcccgat gaatagcatg ttccagttcg cgcacgttcc
     5341 ccggaaaacg atagtgttgc agtaaatttc gcgctccggc acttaatacc acgcgggaga
     5401 gcccctgccg caaacgacac tgctcgcaga aataccccgc cagcagaatg acatcatcgc
     5461 cccgctcacg cagcggcggc accgaaagtg gaaacacgct caggcggtgg aacaagtcag
     5521 cgcgaaatcg ccctgccagc acctcttcgc gtaaatcgcg gttagtcgcc gccagcacgc
     5581 gcacatcgac ccgcaaacta cggtcatcgc caacgcgctg aatatcgcca tactgcaaca
     5641 ccctcagcag cttggcctgc aatgccaacg acaactcgcc gatctcatcc agaaacagcg
     5701 tgccgttatc cgccatttca aacttcccac tgcggttact gattgcgcca gtaaacgctc
     5761 ctttcacatg cccgaacaac tcactttccg ccacactttc cggcagtgcg gcacagttga
     5821 gatagaccag cggattcacc gcccgtggcg aggcctcatg aatcgctttc gccaccagct
     5881 ccttaccggt tcccgtctca ccgctgatca ggacgttgag atcggacgcc gccacaatct
     5941 caatctcttt tttcagttgc gtcatgcctg gcgacagacc gatcatctgc gtctgtttca
     6001 ccgcttcaaa tcgagcggca tcgcccggca gcatattctg gctttccagt tgctcaatca
     6061 gcaacgcatt gcttaacgct cccgccgcca gcgcggcaat caggcgtaac tcttcgtcgc
     6121 tgaaaacatc gaactgatcg ggctgcatcc cgtcgagcgt caatgcgccg atcaggtttt
     6181 gcccggcaaa cagcggcaga ccaacgcagg cgtgaacctt cagactctcc tgccctggaa
     6241 tcaaaccgtc atagggatcg ggcaattcgc tgtctgcggg aaagcgcacc acatcacccg
     6301 cgcgggcaat cgcttccagc cgtggatgac cttccagcgc aaagcgtcta ccgagtacat
     6361 ccttcgccag cccgtcgatg gcaagcggaa taaactgccg cgagtcgtaa cgtagcaacg
     6421 cagacgcatc gcactccagc acctgacgca gcgtggtgat caggcgctga aaacgatcct
     6481 gatgaccaat cccacgctgc aattcgatgg cgatattcgc cagcacatca acggaaaaac
     6541 tcatctttgc ctcactgtca atttgactat agatattgtc atatcgacca tttgattgat
     6601 agtcattttg actactcact aatgagcata attttattta tagagtaaaa acaattagat
     6661 aaaaaactgg cacgcaatct gcaattagca agacatcttt ttagaacacg ctgaataaat
     6721 tgaggttgct atgtctattg tggtgaaaaa taacattcat tgggtgggtc aacgtgactg
     6781 ggaagtgcgt gattttcacg gcacggaata taaaacgctg cgcggcagca gctacaacag
     6841 ctacctcatc cgcgaagaaa aaaacgtgct gatcgacacc gtcgaccata aattcagccg
     6901 cgaatttgtg cagaacctgc gtaatgaaat cgatctggcg gatatcgatt acatcgtgat
     6961 taaccatgca gaagaggacc acgccggggc gctgaccgaa ctgatggcac aaattcccga
     7021 tacgccgatc tactgtacag ccaacgctat cgactcgata aatggtcatc accatcatcc
     7081 ggagtggaat tttaatgtgg tgaaaactgg cgacacgctg gatatcggca acggcaaaca
     7141 gctcattttt gtcgaaacac caatgctgca ctggccggac agcatgatga cttacctgac
     7201 aggcgacgcg gtgctgttca gtaacgatgc tttcggtcag cactactgcg acgagcatct
     7261 gttcaacgat gaagtggatc agacggagct tttcgagcag tgccagcgtt actacgccaa
     7321 tatcctgacg ccgttcagcc gcctggtaac gccgaaaatt accgagatcc tgggctttaa
     7381 cttaccagtc gatatgatag caacctccca cggcgtggtc tggcgcgata acccgacgca
     7441 aattgtcgag ctgtacctga agtgggccgc tgattatcag gaagacagaa tcaccatttt
     7501 ctacgacacc atgtcgaata acacccgcat gatggctgac gctatcgccc aggggattgc
     7561 ggaaaccgac ccacgcgtgg cggtgaaaat tttcaacgtc gcccgaagcg ataaaaacga
     7621 aatcctgacc aatgtcttcc gctcaaaagg cgtgctggtc ggcacttcga cgatgaataa
     7681 cgtgatgatg ccgaaaatcg ccgggctggt ggaggagatg accggattac gcttccgtaa
     7741 caaacgcgcc agtgctttcg gctctcacgg ctggagcggc ggtgcggtgg atcgtctttc
     7801 cacgcgcctg caggatgcgg gtttcgaaat gtcgcttagc ctgaaagcga aatggcgacc
     7861 agaccaggac gctctggagt tatgccgtga acacggtcgc gaaatcgccc gtcagtgggc
     7921 gctcgcgccg ctgccgcaga gcacggtgaa tacggtagtt aaagaagaaa cctctgccac
     7981 cacgacggct gacctcggcc cacggatgca gtgcagcgtc tgccagtgga tttacgatcc
     8041 ggcaaaaggc gagccaatgc aggacgttgc gccaggaacg ccgtggagtg aagtcccgga
     8101 taacttcctc tgcccggaat gctccctcgg caaagacgtc tttgacgaac tggcatcgga
     8161 ggcaaaatga gcaacggcat tgtgatcatt ggttcgggct ttgccgcccg ccaactggtg
     8221 aaaaatattc gcaaacagga cgccactatt ccattaaccc tgattgccgc cgacagcatg
     8281 gatgagtaca acaaaccgga tctcagccat gttatcagcc aggggcaacg tgccgatgac
     8341 cttacccgcc agacggcggg tgaatttgcc gagcagttta atctgcgcct gtttccgcac
     8401 acctgggtga tgaatatcga tgccgaagcc cgtgtggtga aaagccagaa taatcagtgg
     8461 caatacgaca agctggtgct ggcaactggt gccagcgcct ttgtcccgcc tgtgcctggg
     8521 cgtgagttaa tgctgacgtt aaatagtcag caagagtatc gcgcctgtga aacgcaactg
     8581 cgggatgccc gacgcgtgtt gattgttggc ggtggtttga ttggtagcga actggcgatg
     8641 gatttttgtc gtgcaggcaa agcggtcacg ctaatcgaca acgctgccag tattctggcg
     8701 tcgttaatgc caccagaagt aagcagccgc ttgcagcatc ggttgacgga gatgggcgtt
     8761 catctgctgt tgaaatctca gttgcagggg ctggaaaaaa cggattctgg cattctggca
     8821 acgctggacc gccagcgtag catcgaagtg gatgcggtaa ttgccgccac cggactgcgc
     8881 ccggaaaccg ccctggcacg acgcgccggg ctgacgatta atcgcggcgt ttgcgtcgat
     8941 agttatctgc aaaccagtaa tgccgatatt tacgcgctgg gcgattgcgc ggaaattaac
     9001 ggtcaggtac tgccgttcct gcagccgatt caacttagcg cgatggtgct ggcaaaaaat
     9061 cttctcggca ataacacgcc gctgaaacta ccggcgatgc tggtgaaaat caaaacgccg
     9121 gaattaccgc tgcatctggc aggcgaaacc cagcgtcagg atttacgctg gcagattaat
     9181 accgaacgcc agggaatggt ggcgcgcggt gttgacgatg cagaccaact tcgcgccttt
     9241 gtggtcagtg aggatcggat gaaagaggca tttggattgt tgaaaacatt gccggtgtag
     9301 gcgggctact gtgcccaaaa tgtcggatgc gacgctgacg cgccttatcc gacctacggg
     9361 gagcgcatgt gttggtcaca taatgcgttc atgtcgcatc cgcctactgt gcccaaaatg
     9421 tcggatgcga cgctggcgcg tcttatccga cctacgggga gcgcatgtgt tggtcacata
     9481 atgcgttcat gtcacataca gctactgtgc ccaaaatgtc ggatgcgacg ctggcgcgtc
     9541 ttatccgacc tacggggacg catgtgtagg ccggataagg cgtttacgcc gcatccggca
     9601 atggtgtcca aatgcaacac gatttatccg ttctggactt cacccgctaa ccaacgcgcc
     9661 gcagcaataa ccccctgccc cagagacaaa ccgccatcac ccgccggtaa actctgtgga
     9721 aagagcaatg tgaaatcagc gagataatgc gccagacgtg cacgcagcaa acggttatga
     9781 ataaccccgc cgctaaatac cagcgtagtg ataccacgca tcgtggcctg ctcacgcatc
     9841 aacgcggcaa aaccctgcgc cagcgcatca tgaaacgccc acgcgcgttg attaaccggt
     9901 gcctgccagt tcagccactg ctgccagaaa gtggtgagat ccagttgatt gtccaccagc
     9961 ggcattgtca ccggatgcgt cactccgtgg catgaggctg cgagcgcctc cagagcacaa
    10021 gccgcttcac cttcataact taacgtggct ggcgcacagc ccagtgccgc cgccactgca
    10081 tcgaacaccc gcccacacga tgacgccagc ggcgcgttaa ttccacgctc aatggcccgc
    10141 gccagaacgc tccagttttg ctgttgtaca cttgccgttt cagggtaatt ctgccactcc
    10201 ggcacaaagc gcaggcactg cgccagcagg ttacgccacg gctgcttcgc cgccaaatcg
    10261 ccacccggaa gcgccactgc aggcaagccg cccaggtgct cacattcgcg atagttcacc
    10321 cgcaggcact cgccgcccca caaagcgccg ttctccccca taccgatacc gtcgagcgtc
    10381 aaagcaatga catcaccgcc ctccagtggc cagtgatgtt ctgccagaca cgccgccgcg
    10441 tgggcatgat gatgcagcac cgtttgcgtc ggcagattca tttcgcgcgc ccactggctg
    10501 gagacatagc ccggatgtgc gtcatgcaca acgtattgcg gggtgaaatc gtagatgttt
    10561 tgcatcaggc gtaacgcttc gcgccactgc atctggatgc catcgtcact taaatcgccc
    10621 agatgctggc tcaacaccgc ttgttcaccg cgcaccaggc agaaggtatt tttcagatcc
    10681 gcgccgagac acagcacagg cggaacattt ttaaagcccg gaggcaaagc cagcgcatcc
    10741 ggcacatacc cccgcgaacg gcgtagcatt tcgccgcttt cgcgcaccac cgaatcatcc
    10801 atccgctgca cgatgtcgcg gttatgtatc aagaatccgt cggcaatgcc ctgcaaatct
    10861 gccagcgcct gttcgttgct gatagccggt ggtttaccgc tcaggttgcc ggaggtcatc
    10921 accagcgggc attgcagttc ctgtaacagc aaatgctgga gcgggttcgc aggcaacatt
    10981 accccgactt cgttaaggtc aggggcgata tcatcacaaa gctcaggtac gtatttttta
    11041 tccaccagca caatcggcgc ggcgggcgtg gtaagcaact ggcgcgcagc gtctggtaaa
    11101 ccgtcagcca ctggcaacat gaccgccagc ggtttcgccg ggcgatgttt gcgcgcccgc
    11161 agtgtcgcca ccgcgttact gttacgtgca tcgcaggcaa gatgaaatcc gccaatccct
    11221 ttgatggcga caatgttgcc catttttaac tgtgcgatag ccgcctgtaa tgccgcctct
    11281 tgttctgcat gttcaccatg acttacccat tcaagatgcg ggccacactc cgggcaggcc
    11341 accggctggg cgtggaagcg acgatcgagc gggtcgcggt actctttatc acaggctgga
    11401 cacagcggaa acgccgccat cacggtaaac gggcgatcgt aaggcatggc gcgaataatg
    11461 gtgaaacgcg gaccgcagtg ggtacagttg ataaacggat aacgccagcg tcgctcgcca
    11521 ggggtattca tttcggcaag gcaggcaggg caagtggcgg catcgggaac aatttgcgta
    11581 ttcatggcgc cgcccgcgct ctggcggata gtgaactcgg tgggcagttg tgaccagata
    11641 aacggctcac gctcgacgct atcaatacgc gccagcggcg ggcagtgctg atgcaattga
    11701 acaagaaacg tttccgggtc ttccagcagc cggacttcta cgccatcgcc gtcattacag
    11761 acatcgccgt gaagatttaa ttgctgtgcc agctgccaga caaacggacg aaaaccgacg
    11821 ccctgcactt tgccacgaat acgcagttgg acaccgcaag atgtgttttt tgccattgag
    11881 ttattcccgc catcatgaat tgcgtaaccc gtccagccgg acacgacagc gtcgcatccg
    11941 gtagtcacag gtcggcgata ctgcccgctt cgtattctac gaatatttcc gggaattcct
    12001 ttgatgccag aacagttctg taagattttt agaacatcag cgccgtgcgg cgacgttttt
    12061 ctgcgctcag ttgttcaagt ttattacgat cgacacaaat cagcgcatga gtcgggcaag
    12121 ccgccataca cgccggaccg tcttcacgat ggttgcacag gtcgcattta ttggcttcgg
    12181 ctttgtcagc ccgtacattc agccctgcgc cgctgttgcg aatcaccgga cgtaccacca
    12241 cttccatcgc gccatacggg caagccacaa cgcaggtttt gcaaccaatg caacgttcct
    12301 gcatcacatg aacaaaccct ttatcacggc tgatagcacc attcgggcag acgttagcgc
    12361 acggtgcatc ttcacactga cggcaaactg tcgccgtgga aatgttcaca cctttaatga
    12421 catggatacg cggtaaaaat gtttccgggg tcagcgatgc acagtcctga ttttcctgat
    12481 gagaaaccac gcacgccact tcacaggtac ggcaaccaat acatttactc gcgtcagcaa
    12541 tgatgaaacg gttcatcaaa ttctccagca atgacagtta atgcgccgat acattcacaa
    12601 atcatgccag tttttaattc actgttattt aaggaaatta atttctgtaa tgcaggaaaa
    12661 aaagatgtca tcgacactag tgacgatgac atgtgatgac aatgtttatc gcgaaagagc
    12721 aatgagtgag tcgcggcgga tcagttttcc gctgaaggtt ttcggcggtg agaaatcccc
    12781 gccatcgagc ataaaaatca gccgtccaat aatttcctga atcatctctg ttaccggaat
    12841 ttttacgctg gagagcgccg gaacggtgta gggggcaata gcgatatcat cgaatcccat
    12901 aactgacacc tgctctggca ccgctacgcc gcgctcgtgt aacgctttca tcgcacctat
    12961 cgccatatcg tcgttactgg caactaacgc gctaaattta gccccacgtt cgagcaacat
    13021 ttctacccct tcggccccgc tggcaggcgt ccatttaccg ttagcgataa gtttttcatt
    13081 gagcgcaata ccatgctgcg acagcgcgtc tttatacccg gcaagacgtt caatgctggt
    13141 gggggaatcc atcgagccgg taaggaaagc aatctcctga tgtccggcgt ttatcaactc
    13201 tgccacggcg ttaaagctgg tctgtttatg atcgcaccag acgctatggc tgctgttttt
    13261 gcgcaggcgg cgattgagca ccattatcgg ctgactgtgc gcgtcaatga tgtcatcgat
    13321 ctcatccacg cttaaaaaac gcgggtaaat cataatcgcg tcgcagcgca aatccagcag
    13381 atactgaatc gcctggcgct cttcttcggc gctgtgttta ccatctgcca atagtaactg
    13441 tcgccctttc tcttccgcca ttcgcgcggc atgaaacagt aattcactaa aataaatgcc
    13501 gtggtaaagc gtgttggtca ctaccagccc cagcgtctga gtactcttcg ccgacagatt
    13561 acgcgccagc aagtttggac ggtaaccgct ctcttctacc gcctgaaaca cgcgatcttt
    13621 agtctcctgg ctgacgtagc cattacctga aagcacgcgg gaaacggtcg cttttgaaac
    13681 cccggcgcgc ttcgccactt ccagcatcgt cgtcatcatt ttcatccctt tacacgcaat
    13741 caacgcagtg tactgcaccg tttgccgatt gtccttgcgc aatcggcggg aaaaatattc
    13801 aggtgaccgg tttcacaaat ataaaaaatg aacaattaac tctcttgctt atttggtgac
    13861 aactattcat gattttgtga aaccggtttc ttaattccgt ttcagcatcg gcatttttcc
    13921 gtcacgtcga ctgataacaa ccacatctac cctactgata acaggataaa atccgatggc
    13981 caaaaattat gcggcgctgg cacgctcggt gatagcggca ctgggcggcg ttgataacat
    14041 ctcggcagtc acgcactgta tgacgcgttt gcgctttgtt atcaaagatg atgcgcttat
    14101 cgacagcccg acgttaaaaa ccatccccgg cgtgctcggc gtggtacgca gtgacaacca
    14161 gtgtcaggtg attatcggca ataccgtttc acaagccttt caggaaatcg tcagcctgct
    14221 gccgggagat atgcagcccg cacagcccgt aggtaaaccc aaactcacgc tacgtcgcat
    14281 tggtgcgggg atcctcgatg cgctgatcgg caccatgtca ccgctgatcc cggcgattat
    14341 cggcggatcg atggtcaaac tgctggcgat gatcctcgag atgagcggcg tgctgacaaa
    14401 aggatcgccg accttaacca ttctgaatgt gattggtgac ggtgctttct tcttcctgcc
    14461 gctaatggtc gcggcatctg ccgccatcaa atttaaaacc aatatgtcgc tggcgattgc
    14521 catctcgggt gtgctggtac atccgagttt tattgaactg atggcgaaag cggcccaggg
    14581 ggaacatgtc gaatttgccc tgatcccggt caccgcggtg aaatacacct acacggtgat
    14641 cccggcgctg gtgatgacct ggtgcctgtc atatatcgaa cgctgggtgg atcgcattac
    14701 accggcggtg accaaaaact tcctcaagcc aatgctgatt gtgttgattg ccgcaccgct
    14761 ggcaatcctg ctgattggcc cgattggtat ctggatcggt agcgccattt cggcgctggt
    14821 ttacaccatt catggttatc tgggctggct ttcagtcgcc attatgggcg cgctgtggcc
    14881 tctgctggta atgaccggga tgcaccgcgt ctttacgcca accatcattc agaccattgc
    14941 cgaaaccggc aaagaaggga tggtcatgcc gtcagagatc ggcgctaacc tgtcgctggg
    15001 cggttcatca ctggcggtgg cgtggaaaac gaaaaacccg gaactgcgcc agactgcgct
    15061 ggctgcggcg gcatcagcca ttatggcggg gatttccgaa ccggcgttat acggcgtggc
    15121 gatccgcctg aaacgtccgc ttatcgccag tcttatcagc ggttttattt gcggcgcggt
    15181 tgccggtatg gcggggcttg ccagccactc aatggcagcg ccggggctat ttaccagcgt
    15241 gcagttcttc gatccggcga atccaatgag catcgtctgg gtgttcgcgg tcatggcgct
    15301 ggcggtggtg ctgtcgttta tcctcacact gttgctcggc tttgaggata ttcctgttga
    15361 ggaagcggct gccgaggcgc gaaagcatca gagcgtacaa ccgaccgtcg ccaaagaagt
    15421 aagtcttaat tgaggatgaa aatgtcagta tttccagaag gttttttatg gggcggcgcg
    15481 cttgccgcca accagtctga aggtgcgttc cgtgaaggtg gcaaaggact gacaacggtc
    15541 gatatgatcc cacacggcga gcatcgaatg gcggtgaaac tggggctgga aaaacgtttt
    15601 cagttgcgcg atgacgagtt ttatcccagc catgaggcga cgggttttta tcatcgttat
    15661 aaagaagata tcgccctgat ggcagagatg ggattcaagg ttttccgtac ctcaattgcc
    15721 tggagccgtc tctttccgca gggcgatgaa atcacgccca atcagcaggg cattgctttt
    15781 tatcgttctg tctttgaaga gtgtaaaaag tacggtatcg aaccgctggt cacgttgtgc
    15841 cacttcgatg tgccgatgca tctggtcacc gaatatggct cctggcgtaa ccgcaagctg
    15901 gtggagtttt tcagccgcta cgcccggacc tgctttgaag catttgatgg tctggtgaaa
    15961 tactggctaa ccttcaatga aatcaacatt atgttgcata gcccgttctc cggcgcgggt
    16021 ctggtgtttg aagaaggtga aaatcaggat caggtgaaat atcaggccgc gcatcaccag
    16081 ctggttgcca gtgcgctagc caccaaaatc gcccatgagg ttaacccgca aaatcaggtg
    16141 ggctgtatgc tggcgggcgg caacttctac ccttacagtt gcaagccgga agatgtctgg
    16201 gcggcgctgg agaaagatcg ggaaaacctg ttttttatcg atgtgcaggc gcggggcacg
    16261 tatccggctt actctgcccg cgtattccgc gaaaaagggg taaccatcga caaagcaccg
    16321 ggcgatgatg aaatcctgaa aaacaccgtc gattttgtct ctttcagcta ttacgcctcg
    16381 cgctgcgcct cggcggagat gaacgccaac aacagcagtg cggcgaacgt ggtgaaatcg
    16441 ctgcgtaacc cgtatctgca agtgagcgac tggggctggg gaattgatcc actcggtctg
    16501 cgtatcacca tgaacatgat gtacgaccgc tatcagaagc cgctgtttct ggtggaaaac
    16561 ggcctcggcg caaaagatga acttgctgcc aatggcgaga ttaacgatga ctatcgcatc
    16621 agctatttac gcgaacatat ccgcgcaatg ggcgaagcga ttgcagacgg cattccgctg
    16681 atgggctaca ccacctgggg ctgtattgat ttagtttccg cctctacggg tgaaatgagc
    16741 aaacgctacg gttttgtctt tgttgaccgt gacgacgcag gcaacggcac gctgacgcgc
    16801 acacgtaaga aatcgttctg gtggtataaa aaagtgattg ccagtaatgg tgaagattta
    16861 gagtaagtaa ccgtgccgga tgcggcgtga acgccttatc cggcctacat agttcgtgcg
    16921 acatgtaggc ccgataagcg cagcgcatcg ggcaatttag catttggcat cagagctggt
    16981 tcaccccatc aagaacatcc ctgtcctgat tccttattga aaaactactc ttcttccacc
    17041 gctaactgcg cgaagccgcc gttcccttcc cagccttcca gtcgttgata aacggtttct
    17101 accgcatctt taatcggctg ggtcatcggg tagtaaaagc cgacgatatc cggctggatg
    17161 ccgaggaaaa tcacttcgcc aatatcttct ttcaactgat cgataaggta gttcaacggc
    17221 atgttatggg tagtcatcat aaacatctcg gcgatatcat ccgggtcgat gatgcggatt
    17281 tcgccggggt ttagccccat atcagtagcg tcaacaatca gtaaccgcgt agggcgcagt
    17341 tcacggatgg cgacgatgtc gttttccggt gcgctaccgc cgtcaatcac cacccagtta
    17401 cctttcggcg cggcggcgca cttttccgcc agcagcggac ctgcgccatc atcgcccatc
    17461 atgctattgc caacacagag taaaacgtca gtcacgcagt ctcctcacca tcaaatagat
    17521 ggcgttttcc tgatgaatat catgcagcat acttaacagc attgtactcc attcctgctg
    17581 ctgcgaagtt tgcgctgccc gggctttatc gaatgcattg gccagcatcg gcacatggtt
    17641 aatgtcgatg acgatctcac catatttcgg cacaccttcc attttgcggc gggcttcgct
    17701 gcccgcctcc agagtggcaa tccatgccag atattcatcc cacgggcagg tgagcgccgc
    17761 ttccaggcaa tcgataaccc caaggtggtg accaatcgcc aggctgtaat agaccacctg
    17821 ctgcgcctcg gcgggcgtgg catcgttctc atcaataaat ttacggctca gttgactgaa
    17881 caccaccttt tcactcatcg gatacgcgcc tcttctacaa catgattcag atggctgaca
    17941 atctcgttca gacgcggatc gttttccgct tccagccagc gtgcaacctg ttcttcgccc
    18001 tgccctaact gtgtaaggta atcatcggca atctgacgac cgtaacgata acccgccagt
    18061 cgacgtgctt cgcgatccac tttcacacgc agcggctgca ccatattgcc atgcagaatt
    18121 tctgccggtt gttcatcctg ctcacccggc ccacgggcgt gaattttctg ctccagcagg
    18181 ccgagcgcca ttgcaaagcc gtacagcgtg gcggcaggcg ttggcgggca gccagggata
    18241 taaacatcca ccgggacgat tttatccgta ccgccccaca cgcagtagag atcgtggaag
    18301 atcccgccgc tgttaccgca ggcaccgtag gagatacaaa ttttcgggtc cggcgcggac
    18361 tgccacgcac gcagcgcagg ggatcgcatt gcacgggtga ccgcgccggt aaacagtaaa
    18421 atatccgcat gacgcggtga aggaacgact ttaatgccga agcgttctgc atcgaacagc
    18481 ggcgacaaag tggcgaaaat ttcgatttcg cagccgttgc agccgccgca gtccacgcgg
    18541 taaacatagg cagaacgttt gatttttttc agtaacgacg ccttcatgct ggcgatggat
    18601 tcatccaccg tcatcgggac cggaatgccg ttggcgtcac gggggcctaa taaattgctc
    18661 atcagatggc ctctttcata tggcgagtca gttcaatacg gtcggacggc accaggcatt
    18721 tctggcgctt acattccggg caagtctcaa agctttcgcg gtggttttcc gcgcggctgt
    18781 cgccgttgtg cttaagcagc gcaatggcgt agtcgatctc tttctggacg gcgaaaggac
    18841 gattgcagac gcgacagttg cacagcgcga agcgggactg ttgcaggaag tcttctttct
    18901 tccacaccgc cagttcgtac tcttgcgaca gcttaatcgc cgccgtcggg cagacttctt
    18961 cgcagcgtcc gcagaagatg caacgcccga gattaaactc ccaggccagc tctcctgtgg
    19021 cgaggtcagt ttcaaccgtt aaggcgtttg acgggcaggc attgacgcag gccgcgcagc
    19081 cgatgcactg ctgcgggttc tgctctggct taccacggaa gtttttatca accgcaatcg
    19141 gctccagcgg ataagacgag gtcgccgtgc cggttttgat gacttttttg ataaaggtaa
    19201 acatggcgat tccttatttc agcggcgagt ttttacgctc aatgctgtaa cgctcgagtt
    19261 ctttgtacgg caccactttg ctcttcttct tacgcacatc gaccacggtc atgcggtcgg
    19321 tacaggagta gcaagggtcg aggctaccga taatcagcgg cgcatcggaa acggtattgc
    19381 cacgcagcat gtagcgcagg gtcggccagt tcgcgtaagt cgcggcacgg cagcgccagc
    19441 ggtacagctt ctggttgtcg ccggtcatgc tccagtggat atcatcgccg cgcggcgctt
    19501 cggcaaagcc cagcgcaaag cggtgcggaa tgtaggtaaa gccttccacc atcagtgggc
    19561 cacccggcag gttatccaga ccgtagtcga tcatgttcag cgcggtatag acttcgttga
    19621 tacgcacttt cagacgggaa ataacgtcgc agccctgctc gctgtggact tccattggca
    19681 gcaggccata accaacaaac gggtgatcgg cgcgagtatc gcgagcgtga ccgctggcac
    19741 ggaccatcgg gccgacgtta ctgaagtcgc gagcgatttc cgggtccaga cgaccaatgc
    19801 cgacagtgcg ttgttccatg ttcggtgtgc tcaacagcac atccaccagc tcctgcactt
    19861 cacgacgcat ctgttgcgcc agctggcggg tctggatcat gtcatctttc agcagatcgc
    19921 gacgaatccc gccgatcagg ttcaggccgt aggttttacg cgcaccggta aggatctctg
    19981 ccattttcat ggaggtttca cgcacgcgga agaactgcat aaagccggag tcaaagccgg
    20041 taaagtggca ggcaagaccg aggttgagca gatgcgagtg caggcgttcc acctccagca
    20101 gaatggcgcg gatcatctgc gcacgttctg gcacctgaat acccatcgcg ttttccaccg
    20161 acgtggtgta ggcggtgctg tgagcaaagc cgcagatccc gcacacacgg tcagagagga
    20221 aggtcacttc gttgtaaccc atacgggttt ccgccagttt ttccatgccg cgatgaacgt
    20281 agaacagacg gtagtcggcg tcgataatgt tttcgccatc gacgaacaga cggaagtgac
    20341 ccggttcgtc agaagtgacg tgcagcggac caatcggcac gacgttgttt ttcttgtcgc
    20401 ccagttcgtt gatgaactcg taggtttcag catcggtggt cggtgccgga cgttgacgat
    20461 aatccatgct gtctttacgc agcggataaa gttcatccgg ccagtcatcc ggcagcacca
    20521 gacggcgttc atccggcagc cctaccggaa tcaaaccgta catatcgcgc acttcgcgct
    20581 cgccccacac cgccgccgga acgcgcggcg tcacggacgg atactccggt ttgttggcgt
    20641 caacttcgac gcgaaccgta atccaacact tagtgccctt ctccatcgac agcacgtagt
    20701 aaacggcgta atgaccattc agtttgcgtt cgtcgttacc aaacagcacc gacagccagc
    20761 caccctgttt gtagtagaga aactccacca cttccggcag atagttcacc ttcacggtca
    20821 cggtcagctg atctttggtc tgccaggcgt ggtccagcac gacgcccgga aatgcctcat
    20881 tcagcgcggc gagataatgt tgacctaatt tttcttcaga catgctcaaa ctctctttaa
    20941 tcacgccgcc agcaaggaga cgaacgctaa aaatgcaaag ccaaacccag cccaggtaat
    21001 gcgcggagta atatcaagac gcagacgggc catgctgttt tcgaacagcg cgataaccag
    21061 gacgccgacc accagtttga cgatggcaat caccagcgcc agcagcagtc caccagcggt
    21121 gaaggtttcc atttgtcccc acggaataaa caccccgacg aacatctgca gcaccaccag
    21181 ctgtttcagg ctgatacccc atttcatgac gccaaagccg ctgccgctat actcagagag
    21241 cggaccttcc tgcaactcct gctcggcttc cgccaggtcg aacggcagtt tgcccatttc
    21301 gataaaagtg gcgaacgcac aggcacaaag cgccagcacc agcgggatgc tctggctcag
    21361 cggccagtga taaacggtgt cggtgatgtt gctgatgttg gtggaaccgg caacctgtgc
    21421 ggcaacccac agaccaagca gcaacatcgg ttcgaccagc acgccaagca tcgcttcacg
    21481 gctcgcgccg atagcggtaa acgggctacc ggtatccaga ccagaaatgg caaagaagaa
    21541 acgcgcgatg gcaaagagat acagtaaggt gatcaaatca cccagttgcg gcagcggaga
    21601 accaacggtc accaccggca gcgcagtagc gatagtcagc atgacgccca ccatcacata
    21661 cggcgtcagg cggaacaccc agccggaggc atccgggcca acgctctggc gacccagcag
    21721 tttgataatg tcgcgatact cctgcaacac gcccggcccg cgacggttat gcaagcgggc
    21781 gcgcgccacg cgggttatac cggagagcag cggcgcaacg gcaaatagca ccagcgcctg
    21841 aattaacgga tataaaacac tcattctcag gctcctcgtg aaacaataat caccaccagc
    21901 accgccagtt caaccagcgc catccggcgg aacagcaacg cactcccctc gcactgccag
    21961 cccggcacca gagacaccgg attcagccat ttgcgtagtt tcagcaccgg cgcaaacgcc
    22021 tgtttcaccg gcatggcaaa accgtgagcg gtaatcacca ttgatttttc gtggtcgtag
    22081 ccgcacaccc aggccgcgcc gcgggaacgc gatggcaaac gatcgccttt gcaaatcgcc
    22141 ataatgatga atggcagcag cgggcaggca atcagcagca acgtgatcat tggttgagaa
    22201 acggtggtgt tagcaggctc cagaggcaga ggtactgcag cagagagcat cggcagcagc
    22261 cacggcgcag caacaccgcc aattacgcag caaatcgcca gtgccactac gcttacgctc
    22321 atcaggagcg gtgcacaggt ggcgttttcg gcttctttgg tacgcggcgc gccgaggaac
    22381 gtgacgccat agactttcgc catacacatc accgccagcg caccggtaat tgccagcccc
    22441 acagcgagca gcggccccag cagacgggca acaaacgcgc cactattgct cagtttgaaa
    22501 aaggattgat agataaccca ttccccggca aacccgttca gcggcggcag cgcagccatt
    22561 gccatcaacc cgactaacat ggcgatggag ataaccggca ttttcttgcc aataccaccg
    22621 agtttttcga tatcgcgatg accggtacgg aaccagacgc tccccgcccc gaggaacagt
    22681 acgcttttga acaggctatg gttaagcaga tggtacagac caccgaccag accaagggca
    22741 atcagcgccg gttgttcgag cgcgataccc gttacgccag cgcccagccc cagcaggatg
    22801 atgccgatat tttccagggt gtggtaagcc agcaggcgct ggatgttgtg ctccatcagc
    22861 gcatacagac caccgacaaa cgcggtgatc atgccgagca ccagcagcgc gatcccccac
    22921 cacagcggtg cattaccgcc cagcagtgac agggttaaaa tgcccagcag gccaattttc
    22981 atgactaccg tagaaaacaa cgcggcagct ggcgcagagg cgttagcatg tgcctgcggc
    23041 acccagccgt gcagcggaat aatcccggcc agcaggccaa agccaatcac accgagcagc
    23101 cagatatcgg aaccgagcgg cagctgttgc atacgcatat ccagcaggcg cagatccagc
    23161 gtgccataac gctgccacag cagccagcaa gcaatcacca gcagcagagt gccaagacgc
    23221 cccagcgcaa accacagttt gccctctttg ctgttgctgg tgaggaacac cgcacacaag
    23281 gccatgactt cggccattac cacgaacatg ccgaggttgc tggcaatgac ggcgcagacg
    23341 gcggcagcca tcaacatatt gatctgcaag ccgttgcact tcacctgcgc gtggcgatgc
    23401 cagtcaatgt tgtagaggct gacaaacaga ccgcacagac cgagcgtaat cagccaaatc
    23461 gcgttaagcg gagagatttg cacatcgtag cttaccagcg acagcgcacc gctcacgcca
    23521 accgcgccag tcagtacagt gaagcccgcg gctgccgtat acagactacc aaccgcgccg
    23581 ccaattccgg ctatccagcc gcttagcgcc ttttgaaaag aaaagagaaa tgccagaaca
    23641 gcggcggcga caaaccacgc cacgccgcta ttgatcaggg aaattgcgct catttagcct
    23701 ctccactttg agcctgctga aacaaggtga gatcgccaaa gtccgtgtta aaggtcagct
    23761 cacgcttacg tttgctgacg cgggcgatat cggtgttatc caccagatgc agggctttag
    23821 tcgggcacat ccgcacgcag gccggacctt gttcatcaaa gctacaaagg tcacatttga
    23881 cggcgatcgc gcgaatacct ggcacccagt caagcaatgt gctgacacgc gccggagcag
    23941 gcggtgccgg tggcgctttc ggggtattgg cgtttgccgg aatatccagc ggacggctgc
    24001 cggaaaattc aattgcgcca aacgggcagg cgatgccgca cagtttgcag cttacgcaca
    24061 ggctttcatt caactgcacg gccccatcaa cgcgggtgat ggcgttaacc gggcagacca
    24121 ccgcgcaggg tgcatcttca cagtggtgac agagctgcgg cgcagattct ttttcattca
    24181 gcatcactct caggcgcggc attgattgca ggccgtgctg gcgatgcgtc tctgaacagg
    24241 cggcctcaca agtgtggcag ccgatacaga gcgtggagtc agcaattaca aaacgattca
    24301 ccaggcattc ctcaggtgat tgtcattttt gacgaaaaca tgccgatgaa atgtcatttt
    24361 cgacactcat cgacacgccc atccccaaac aggcgtaacg cctgcaaaac gggcaaagcc
    24421 tcagctcatg ctgccgggct ttgtcccttt accagttggc ttaaattcac cggcacattg
    24481 ttttcaatgg cggtgtataa gctgtcgtaa acgccagcga ttttttcgag atagtgttcc
    24541 agaacctgtt cgcgatcgcg gatgttgacc ttgccatcaa tcatgccgtc aatgctcagt
    24601 tgcgcctgcg caatccgctg tttgtcttcg ccatcttttg tttcgacgta atactcgatg
    24661 gtgtggctgt taaagccatc cgccagggtg acgtaaatac gaaaatgttc aaaaaggacg
    24721 aaacagagtt ctttgtccgg cgcgcagctc atggcgtgat gcaggcgcgc tttcgataac
    24781 gtgatcccct gaaccagcga attgcagtag atgtgccact ggtcctgtag gcgacgatgc
    24841 cgctgtgcga tgtaatcggc tttctcgctt atttcccaaa tagtcattgt caggttaccc
    24901 gtttaacaga gatgccagct ttaagcattt tttgtgccaa cttttaattt attgttatta
    24961 aagcgatttt taagattttg gtgaatttcg tcgccgtgtc gacgtgtcat ttcgacatca
    25021 tcgacattat tcactgcagg gataatcaac actggcacaa ttattgcttg tagctggcaa
    25081 tagttaatgg gaggcgatat gcacgaaata accctctgcc aacgggcact ggaattgatc
    25141 gaacagcagg ccgcaaaaca cggcgcaaaa cgcgtaactg gggtctggct caaaattggc
    25201 gcattttctt gtgtcgaaac cagctctctt gccttttgtt ttgatctggt ttgccgcggc
    25261 agcgtggcgg aaggttgtaa actgcacctc gaagaacaag aggccgaatg ctggtgtgaa
    25321 acatgccaac agtatgtgac gctactgacc cagcgcgtcc gccgctgtcc acagtgtcat
    25381 ggtgacatgc tacagattgt ggcagacgac ggtttacaga ttcggcggat agaaatagac
    25441 caggagtgag cgatgtgtac aacatgcggt tgcggtgaag gcaacctgta tatcgagggt
    25501 gatgaacata accctcattc cgcgtttcgt agcgcgccat ttgccccggc ggcacgcccg
    25561 aagatgaaaa tcaccggcat taaaacgcct gaatttaccc ccagccagac tgaagaaggc
    25621 gacctgcatt acggtcatgg cgaagcgggc actcacgcgc caggcatgag ccagcgccgg
    25681 atgctggaag tcgaaattga cgtgctggac aaaaacaacc gtctggctga acgcaaccgc
    25741 gcgcgctttg ctgcccgcaa gcaactggtg ctcaacctgg tttccagccc tggttccggt
    25801 aaaaccaccc tgctgacgga aaccttaatg cgcctgaaag acagcgttcc gtgcgcggtt
    25861 attgaaggtg accagcaaac cgtgaacgat gccgcacgca ttcgcgctac cggcacacca
    25921 gcgattcagg tgaacaccgg taaaggctgc catcttgacg cacagatgat tgccgacgcc
    25981 gcaccgcgtc tgccactgga cgataacggt attctgttta tcgaaaacgt tggcaacctc
    26041 gtatgcccgg ccagcttcga tctcggtgaa aaacacaaag tggcggtgct ttccgttacc
    26101 gaaggtgaag acaaaccgct gaaatatccg catatgtttg ccgccgcctc gctgatgctg
    26161 ctcaacaaag ttgacctgtt gccgtatctc aactttgacg ttgagaagtg catcgcctgc
    26221 gcccgcgaag tcaatccaga aattgaaatc atccttattt ccgccaccag cggcgaaggg
    26281 atggaccagt ggctgaactg gctggagaca cagcgatgtg cataggcgtt cccggccaga
    26341 tccgcaccat tgacggcaac caggcgaaag tcgacgtctg cggcattcag cgcgatgtcg
    26401 atttaacgtt agtcggcagc tgcgatgaaa acggtcagcc gcgcgtgggc cagtgggtac
    26461 tggttcacgt tggctttgcc atgagcgtaa ttaatgaagc cgaagcacgc gacactctcg
    26521 acgccttaca aaacatgttt gacgttgagc cggatgtcgg cgcgctgttg tatggcgagg
    26581 aaaaataatg cgttttgttg atgaatatcg cgcgccggaa caggtgatgc agttaattga
    26641 gcatctgcgc gaacgtgctt cacatctctc ttacaccgcc gaacgccctc tgcggattat
    26701 ggaagtgtgc ggtggtcata cccacgccat ttttaaattc ggcctcgacc agttactgcc
    26761 tgaaaacgtt gagtttatcc acggtccggg ttgcccggtg tgcgtactgc cgatgggcag
    26821 aatcgacacc tgcgtggaga ttgccagcca tccggaagtc atcttctgta cctttggcga
    26881 cgccatgcgc gtgccgggga aacagggatc gctgttgcag gcaaaagcac gcggtgccga
    26941 tgtgcgcatc gtttactcgc cgatggatgc gttgaaactg gcgcaggaga atccaacccg
    27001 caaagtggtg ttcttcggct taggttttga aaccaccatg ccgaccaccg ctatcactct
    27061 gcaacaggcg aaagcgcgtg atgtgcagaa tttttacttc ttctgccagc acattacgct
    27121 tatcccgacg cttcgcagtt tgctggaaca gccggataac ggtatcgacg cgttcctcgc
    27181 gccgggccac gttagtatgg ttatcggcac tgatgcctat aattttatcg ccagcgattt
    27241 tcagcgtccg ctggtggtcg ctggtttcga accccttgat ctactgcaag gcgtggtgat
    27301 gctggtggag cagaaaatag cggcccacag caaggtagag aatcagtatc gtcgggtggt
    27361 accggatgcc ggtaacctgc tggcgcaaca ggcgattgcc gatgtgttct gtgtcaacgg
    27421 cgacagcgaa tggcgcggct taggcgtgat tgaatcttct ggcgtgcacc tgacgccgga
    27481 ttatcaacga ttcgatgccg aagcacattt ccgcccggca ccgcagcagg tctgcgatga
    27541 cccgcgcgcg cgttgtggcg aagtcttgac gggcaaatgt aagccgcatc aatgcccgct
    27601 gtttggtaac acctgtaatc ctcaaaccgc gtttggtgcg ctgatggttt cctccgaagg
    27661 agcgtgcgcc gcgtggtatc agtatcgtca gcaggagagt gaagcgtgaa taatatccaa
    27721 ctcgcccacg gcagcggcgg ccaggcgatg cagcaattaa tcaacagcct gtttatggaa
    27781 gcctttgcca acccgtggct ggcagagcag gaagatcagg cacgtcttga tctggcgcaa
    27841 ctgatagcgg aaggcgaccg tctggcgttc tccaccgaca gttacgttat tgacccgctg
    27901 ttcttccctg gcggtaatat cggcaagctg gcgatttgcg gcacagccaa tgacgttgcg
    27961 gtcagtggtg ccattccgcg ctatctctcc tgtggcttta tcctcgaaga aggattgccg
    28021 atggagacac tgaaagccgt agtgaccagc atggcagaaa ccgcccgcac ggcaggcatt
    28081 gccatcgtta ctggcgatac taaagtggtg cagcgcggcg cggcagataa actgtttatc
    28141 aacaccgcgg gcatgggcgc aattccggcg aatattcact ggggcgcaca gacgctaacc
    28201 gcaggcgatg tattgctggt gagcggtaca ctcggcgacc acggggcgac tatccttaac
    28261 ctgcgtgagc agctggggct ggatggcgaa ctggtcagcg actgcgcggt gctgacgccg
    28321 cttattcaga cgctgcgtga cattcccggc gtgaaagcgc tgcgtgatgc cacccgtggt
    28381 ggtgtaaacg cggtggttca tgagttcgcg gcagcctgcg gttgcggtat tgaaatttct
    28441 gaatcagcgc tgccggttaa acctgccgtg cgcggcgttt gcgaattgct gggactggac
    28501 gccctgaact ttgccaacga aggcaaactg gtgatcgccg ttgaacgcaa cgcggcagag
    28561 caagtgctgg cagcgttaca ttcccatcca ctggggaaag acgcggcgct gattggtgaa
    28621 gtggtggaac gtaaaggtgt tcgtcttgcc agtctgtatg gcgtgaaacg aaccctcgat
    28681 ttaccacacg ccgaaccgct tccgcgtata tgctaataaa attctaaatc tcctatagtt
    28741 agtcaatgac cttttgcacc gctttgcggt gctttcctgg aagaacaaaa tgtcatatac
    28801 accgatgagt gatctcggac agcaagggtt gttcgacatc actcggacac tattgcagca
    28861 gcccgatctg gcctcgctgt gtgaggctct ttcgcaactg gtaaagcgtt ctgcgctcgc
    28921 cgacaacgcg gctattgtgt tgtggcaagc gcagactcaa cgtgcgtctt attacgcatc
    28981 gcgtgaaaaa gacaccccca ttaaatatga agacgaaact gttctggcac acggtccggt
    29041 acgcagcatt ttgtcgcgcc ctgatacgct gcattgcagt tacgaagaat tttgtgaaac
    29101 ctggccgcag ctggccgcag gtgggctata cccaaaattt ggtcactatt gcctgatgcc
    29161 actggcggcg gaagggcata tttttggtgg ctgtgaattt attcgttatg acgatcgccc
    29221 ctggagcgaa aaagagttca atcgtctgca aacatttacg cagatcgttt ctgtcgtcac
    29281 cgaacaaatc cagagtcgcg tcgttaacaa tgtcgactat gagttgttat gccgggaacg
    29341 agataacttc cgcatcctgg tcgccatcac caacgcggtg ctttcccgcc tggatatgga
    29401 cgaactggtc agcgaagtcg ccaaagaaat ccattactat ttcgatattg acgatatcag
    29461 tatcgtctta cgcagccacc gtaaaaacaa actcaacatc tactccactc actatcttga
    29521 taaacagcat cccgcccacg aacagagcga agtcgatgaa gccggaaccc tcaccgaacg
    29581 cgtgttcaaa agtaaagaga tgctgctgat taatctccac gagcgggatg atttagcccc
    29641 ctatgaacgc atgttgttcg acacctgggg caaccagatt caaaccttgt gcctgttacc
    29701 gctgatgtct ggcgacacca tgctgggcgt gctgaaactg gcgcaatgtg aagagaaagt
    29761 gtttaccact accaatctga atttactgcg ccagattgcc gaacgtgtgg caatcgctgt
    29821 cgataacgcc ctcgcctatc aggaaatcca tcgtctgaaa gaacggctgg ttgatgaaaa
    29881 cctcgccctg accgagcagc tcaacaatgt tgatagtgaa tttggcgaga ttattggccg
    29941 cagcgaagcc atgtacagcg tgcttaaaca agttgaaatg gtggcgcaaa gtgacagtac
    30001 cgtgctgatc ctcggtgaaa ctggcacggg taaagagctg attgcccgtg ctatccataa
    30061 tctcagtggg cgtaataatc gccgcatggt caaaatgaac tgcgcggcga tgcctgccgg
    30121 attgctggag agcgatctgt ttggtcatga gcgtggggct tttactggtg ccagcgccca
    30181 gcgcattggt cgttttgaac tggcggataa aagctccctg ttcctcgacg aagtgggcga
    30241 tatgccactg gagttacagc cgaagttgct gcgtgtattg caggaacagg agtttgaacg
    30301 cctcggcagc aacaaaatca ttcagacgga cgtgcgtcta atcgccgcga ctaaccgcga
    30361 tctgaaaaaa atggtcgccg accgtgagtt ccgtagcgat ctctattacc gcctgaacgt
    30421 attcccgatt caccttccgc cactacgcga gcgtccggaa gatattccgc tgctggcgaa
    30481 agcctttacc ttcaaaattg cccgtcgtct ggggcgcaat atcgacagca ttcctgccga
    30541 gacgttgcgc accttgagta atatggagtg gccgggtaac gtacgcgaac tggaaaacgt
    30601 cattgagcgc gcggtattgc taacacgcgg caacgtgctg cagctgtcat tgccagatat
    30661 tgctttaccg gagcctgaaa cgccgcctgc cgcaacggtt gtcgctcagg agggcgaaga
    30721 tgaatatcag ttgattgttc gcgtgctgaa agaaactaac ggcgtcgttg ccgggcctaa
    30781 aggcgctgcg caacgtctgg ggctgaaacg cacgaccctg ctgtcacgga tgaagcgact
    30841 gggaattgat aaatcggcat tgatttaaca gatgtggtta tactcgatca acattcaatg
    30901 aaaggaggca gtatggcagt ttcagcaaga aatcaattaa ccggaacagt tagcgcagta
    30961 gcaatgggcg cggtgaatga cgaagtagaa ctgacgctgg caggtggtgc aaagctggtg
    31021 gcaattgtga ctcacagcag ccagcaggct ctgggtctgg cgaaagggaa agaagcgatt
    31081 gccctgatta aagccccgtg ggtaacgctg gcaacggaag actgtggact gaaattctct
    31141 gctcgcaacc agtttgctgg cagcgtgtcg acaatcaccg aaggcgcagt gaacgccact
    31201 gtccatatca aaacggatgc gggttttgaa attgtggcag tggtaacaaa tgaaagtcag
    31261 gatgaaatga aactcaccac tggctcacgc gttattgcgt tgatcaaagc atcagcaatc
    31321 ctgattgcta ccaaagcgta agcgccttat ttgccggaga gtatggctct ccggcagatt
    31381 tatttctttg gacgatattt ttctggcaac tccggcaccg gacgcttgtc atcgatgaga
    31441 tgacgcacgg ttaagattgg atgacgccac agcattctcg gcccggccca acgcataatc
    31501 tgtttcatct cttcacgctt tgcaggctgg taacagtgca ccggacactg cttacaggct
    31561 ggtttctctt cgccgaacac acatttatcc agccgctttt gcgcgtaaac aaacaacgcc
    31621 tcgtaatgct ccggctccgc tgacgcctgc gggcatttcg cttgataaag atcgatcatt
    31681 tttttaatcg tcagtttttc acgagagata cgcttgccgg acatactgcc tccatactct
    31741 taaggtgtat ttatactaca acttaatttg aaaggggact atggctctga aggaggaggt
    31801 ttattcaacc gatacaattt tgcgtacttg cttcataagc atcactcaaa agctgcaaaa
    31861 cagcatcttt cccggaacca gcatcaagaa ctcgccgttc gcttcttccc ctgaaatgat
    31921 taactccggt atcatgtgcg ccttatgtga ttacaacgaa aataaaaacc atcacacccc
    31981 atttaatatc agggaaccgg acataacccc atgagtgcaa tagaaaattt cgacgcccat
    32041 acgcccatga tgcagcagta tctcaagctg aaagcccagc atcccgagat cctgctgttt
    32101 taccggatgg gtgattttta tgaactgttt tatgacgacg caaaacgcgc gtcgcaactg
    32161 ctggatattt cactgaccaa acgaggtgct tcagcgggag agccgatccc gatggcgggg
    32221 atcccctacc atgcggtgga aaactacctc gccaaactgg tgaatcaggg cgagtccgtt
    32281 gccatctgcg aacaaattgg cgatccggcg accagcaaag gtccggttga gcgcaaagtt
    32341 gtgcgtatcg ttacgccagg caccatcagc gatgaagccc tgttgcagga gcgtcaggac
    32401 aacctgttgg cggctatctg gcaggacagc aaaggtttcg gctacgcgac gctggatatc
    32461 agttccgggc gttttcgcct gagcgaaccg gctgaccggg aaacgatggc ggcagaactg
    32521 caacgcacta atcctgcgga actgctgtat gcagaagatt ttgctgaaat gtcgttaatt
    32581 gaaggccgtc gcggcctgcg ccgtcgcccg ctgtgggagt ttgaaatcga caccgcgcgc
    32641 cagcagttga atctgcaatt tggcactcgc gatctggtcg gttttggcgt cgagaacgcg
    32701 ccgcgcggac tttgtgctgc cggttgtctg ttgcagtatg cgaaagatac ccaacgtacg
    32761 actctgccgc atattcgttc catcaccatg gaacgtgagc aggacagcat cattatggat
    32821 gccgcgacgc gtcgtaatct ggaaatcacc cagaacctgg cgggtggtgc ggaaaatacg
    32881 ctggcttctg tgctcgactg caccgtcacg ccgatgggca gccgtatgct gaaacgctgg
    32941 ctgcatatgc cagtgcgcga tacccgcgtg ttgcttgagc gccagcaaac tattggcgca
    33001 ttgcaggatt tcaccgccga gttacagccg gtactgcgtc aggtgggcga tctggaacgt
    33061 attctggcgc gtctggcgtt gcgtactgct cgcccgcgcg atctggcccg tatgcgccat
    33121 gctttccagc aactgccgga actgcgcgcg cagttagaaa atgtcgatag tgcaccggta
    33181 caggcgctgc gtgagaagat gggcgagttt gccgaactgc gcgatctgct ggagcgggca
    33241 atcatcgaca caccaccagt gctggtacgc gacggtggtg ttatcgcacc gggctataac
    33301 gaagagctgg atgagtggcg ggcgctggct gacggcgcga ccgattatct ggagcgtctg
    33361 gaaatccgcg agcgtgaacg taccggcctg gacacgctga aagtcggatt taacgcggtg
    33421 catggttatt acattcagat cagccgtgga caaagccatc tggcccccat caactacatg
    33481 cgtcgccaga cgctgaaaaa tgccgagcgc tacattattc cagagctaaa agagtacgaa
    33541 gataaagttc tcacctcaaa aggcaaagca ctggcactgg aaaaacagct ttatgaagag
    33601 ctgttcgacc tgctgttgcc gcatctggaa gcgttgcaac agagcgcaag cgcgctggca
    33661 gaacttgatg tgctggttaa cctggcggag cgggcctata ccctgaacta cacctgcccg
    33721 acttttattg ataaaccggg tattcgcatc accgaaggcc gccatccggt ggttgaacag
    33781 gtgctgaatg agccgtttat cgccaacccg ctgaacctgt cgccacagcg tcgcatgttg
    33841 atcattaccg gtccgaatat gggcggtaaa agtacctata tgcgccagac cgcactgatt
    33901 gcgctgatgg cctacatcgg cagctatgta ccggcacaaa aagttgagat tggacctatc
    33961 gatcgcatct ttacccgcgt aggggcagcg gatgatctgg catccgggcg ttcaaccttt
    34021 atggtggaaa tgaccgaaac cgccaatatt ttacataacg ccaccgaata cagtctggtg
    34081 ttaatggatg agatcgggcg tggaacgtcc acttacgatg gtctgtcact ggcatgggcg
    34141 tgcgcggaaa atctggcgaa taagattaag gcattgacgc tgtttgctac ccactatttc
    34201 gagctgaccc agttaccgga gaaaatggaa ggcgtcgcca acgtgcatct cgatgcactg
    34261 gagcacggcg acaccattgc ctttatgcat agcgtgcagg atggcgcggc gagcaaaagc
    34321 tacggcctgg cggttgcagc tctggcgggc gtgccaaaag aggtgattaa gcgcgcacgg
    34381 caaaaactgc gtgagctgga aagtatttcg ccgaacgccg ccgctacaca agtggatggt
    34441 acgcaaatgt ctttgctgtc agtaccggaa gaaacctcgc cagcggtcga agctctggaa
    34501 aatctcgacc cggattcact gactccgcgt caggcgctgg aatggattta tcgcttgaag
    34561 agtctggtgt aataacaatt cccgatagaa aaatactatc gggaatatta tctacagctg
    34621 acgaatcaaa taaaaatacc ctgtataata ggaaagttta ttttacaggg taaaaccatg
    34681 ccatctacac gctatcaaaa aatcaatgcc catcactatc gccatatatg ggtcgttggt
    34741 gatattcatg gtgaatatca gttattacaa tcccgcttac atcaactctc ttttttcccc
    34801 gaaaccgact tacttatttc tgtcggcgat aatattgatc gtgggccgga gagtcttgac
    34861 gtcctgcgcc tgctaaacca gccctggttt atctccgtta aaggcaacca cgaagcaatg
    34921 gcgctggatg cgttcgcgac cggcgatggc aatatgtggc ttgccagcgg tggtgactgg
    34981 ttctttgagt taaatgatat ggaacaaaaa gaagctatag gtctattgct gaaatttcat
    35041 caacttccac atattattga aattaccaac gacattataa aatatgtcat cgcacatgcg
    35101 gattatccgg gcaatgaata tctctttggc aaacaaatag cggagagcga attactttgg
    35161 cctgttgatc gtgtgcagaa atctcttaat ggcgagttac aacaaataaa cggcgctgat
    35221 tattttatat ttggacatat gatgcttgat aacattcaga cgttcgctaa ccagatttat
    35281 attgataccg gatcgccgaa aagcgggcgg ctgtcatttt ataaaataaa gtaatgaggc
    35341 cgggtaaagc aaagccacta cccggcactt tttattagcg cttaccttcc gccagcagcg
    35401 gcgggatgct cggcaccatt ggcgcgtcat caatatcttt ctgcgtcatg cggaacgctt
    35461 cgggataatg ttcgcggctg gtacggcgca gcggttcggt atcgcgccag gtataaaggc
    35521 aatgctggca ctgatatacc gtccagacat ctttcaccgg cgatttcgcc atcacttcaa
    35581 tctgttcatc ggcacaacgt ggacaaatca tcttgttctc cttatttacg tgcggccagc
    35641 atagcggtca gtttttcagc ccaggctttg gtttccggca aatccaccac cggctggctg
    35701 tagtgaccac ggttgtccgg ggcgacaggc gtggtggcgt cgataatcag cttgtcggtg
    35761 atccccgccg ggcttgagcc tgggtcgagt tccagcacgg acatattcgg caactgcacc
    35821 aaatcccctg ccggatttac tttcgaggag agcgcccaca tcacctgcgg caggttgaac
    35881 gggtcaacgt cttcatcgac cataatcacc atctttacgt agcccagacc gtgcggcgtg
    35941 gtcatcgcac gcaggcccac cgcgcgggca aagccgccgt agcgtttttt ggtggagata
    36001 atcgccagca ggccgtgggt gtacatggcg tttaccgcct gcacttccgg gaactcggct
    36061 ttcagttgct gatacagcgg cacacaggtg gctggcccca tcaggtagtc gatttcggtc
    36121 cacggcatgc cgaggtacag cgattcgaaa atcggcctgg tgcggtaaga gactttatcg
    36181 atgcgcacca cggtcatgtt gcgcccgccg gagtagtgcc cggtaaactc accgaacggc
    36241 ccctcgattt cgcgtttacg gctttcgatg accccttcga ggatcacttc tgaaccccac
    36301 ggcacatcaa aaccggtcag cggggcggtg gcgatcgggt acgagctttc acgcagcgcg
    36361 cctgccattt cgtactcaga ttgatcgtat ttcagtggcg ttgcgcccat cagcgtgatg
    36421 atcggatcgt tacccagggt gatggcaatc ggcagatctt caccgcgctc ttctgcttta
    36481 tgcagatgca gggcgatatc gtgcatcggc accggttgca ggccgagctt acgcttgccc
    36541 ttcacttcca tgcggtagat gccgacgttc tgcttgccga agttatccgg gtcgagcgga
    36601 tcgcgggaaa ccacgcacgc tttgtcgaga tagaaaccgc catcaccgtc gtttaaacga
    36661 aacagcggca ggatatcgaa caggttgatc tcgtcgccat cgacggtgtt ctgcgcccag
    36721 gctggattgg cgcggcgctc cggggcgatc gggaagttat cccagcggcg gataaactca
    36781 tcaatctgct ttttgaccgg agtgtttggc ggcaggccga gggaaatcgc gtggttctgc
    36841 caggaaccga tggtattcat cgccacgcgg gcatcggtaa aaccgcgaat attatcaaac
    36901 cacagcgccg gtgcaccatc gccgatacgc ccggtggcgt tggcagcggc agccagatcc
    36961 ggttccgcgt tcacctcttc gctgattttc agtaactggc cgtggtcatc aagcgcctgt
    37021 aaaaagctgc gtaaatcatc aaatgccatt attcattctc ctgagaaaaa ttccgggcct
    37081 gcggcaatcc ttgccagcgc ctggcgtggg gatgttcgag gccaaattga tccagcacgc
    37141 gggctactac atggtggaca atgtcatcta ccgtttcggg atggttataa aaggcaggca
    37201 tcggcggcac catcgccacg cccatgcgtg aaagtgcgag catattttcg agatggatgg
    37261 tgctaagcgg catttcacgc ggcaccagca ccagtttgcg gccttctttg agcacgacgt
    37321 ccgccgcgcg ccctaccagg ccatcagcgt aaccagcgcg gataccggcg agcgttttca
    37381 tactgcacgg aataacgatc atgccgtcgg tacgaaagga acctgaggag atggtcgccg
    37441 cctgatccgc cgggttatgg ctgaagtcag cgagggcagc aacatcgcgg gcgctgtaag
    37501 gcgtttccag ttcaatggtg gttttcgccc acttcgacat caccagatga gtctcgacat
    37561 tcggcatctc ccgcagcgct tgcagtaatg ccacaccaag aggcgcaccg gtagcccctg
    37621 tcatcccgac gatcagtttc attgcttatt ctcttaattg ttcgtatacg aacgttatta
    37681 ttaaaatacg cctctgttct catcgcggca agcactgaaa agtaacaaca tccccttcgt
    37741 cactgaaaaa ccgctatgat agcggcagag aattagcccg gagtttacat ggcgttacga
    37801 aataaagcgt tccatcagtt acgacagctt tttcagcagc acacatctcg ctggcagcat
    37861 gagctacctg gcctgaccaa accgcagtac gcggtgatgc gcgccattgc tgataagcct
    37921 ggtatcgaac aggttgcgct gatggaagca gcagtcagca ccaaagcaac gctggcagaa
    37981 atgctggcaa gaatggagaa tcgtggctta gtcagacgtg atcatgatgc cgctgacaag
    38041 cggcgtcgct ttgtctggct gactgccgaa ggagaaaaga tacttgctgc ggcgataccg
    38101 attggtgaca gcgtggatga ggaatttttg gggcgtttga gtgcagaaga gcaggagctg
    38161 tttgtgcagc tggtgcgcaa gatgatgaac acataggatg caaattgccg ggtaggacgc
    38221 tgacgtgtct tatccaggcg acaaaaacac aagcttccca aacaaaagaa aaaggccagc
    38281 ctcgcttgag actggccttt ctgacagatg cttacttact cgcggaacag cgcttcgata
    38341 ttcagcccct gcgtttgcag gatttcgcgc aaacggcgca ggccttcaac ctgaatctgg
    38401 cgaacacgtt cacgggtgag gccaatttca cgacctacat cttccagtgt tgccgcttcg
    38461 taccccagca aaccgaatcg acgtgccagt acttcacgct gtttggcgtt cagctcgaac
    38521 agccatttga cgatgctctg cttcatatcg tcatcttgcg tggtatcttc cggaccgttc
    38581 tctttttcat cggccaggat gtccagcaac gctttttcgg aatcaccacc cagcggggtg
    38641 tctaccgagg taatgcgctc gttaagacga agcatacggc tgacgtcatc aactggctta
    38701 tccagttgct ctgcgatctc ttccgcactt ggttcatggt ccagcttatg ggacaactca
    38761 cgtgcggttc gcaggtaaac gttcagctcc tttacgatgt gaatcggcaa acgaatagta
    38821 cgggtttggt tcataatcgc ccgttcaatc gtctggcgaa tccaccaggt tgcgtatgtt
    38881 gagaagcgga aaccacgttc cgggtcaaac ttctctaccg cgcggatcag ccccaggttg
    38941 ccctcttcga taaggtccag caacgccaga ccacgattgc cataacggcg ggcaattttt
    39001 accaccagac gcaagttact ctcgatcatc cggcggcgag aggcgacatc tccacgcagt
    39061 gcgcgacgcg caaaataaac ttcttcttcg gccgttaaca gtggtgaata accaatctca
    39121 ccaaggtaaa gctgagtcgc gtccaacaca cgctgtgtgg ctccctgcga taacagttcc
    39181 tcttcggcca aatcgttatc actgggttcc tcttctacta aggccttttc gtcaaaaacc
    39241 tcaactccgt tctcatcaaa ttccgcatct tcatttaaat catgaacttt cagcgtattc
    39301 tgactcataa ggtggctcct acccgtgatc ccttgacgga acattcaagc aaaagcctgg
    39361 ttccgccgat ttatcgctgc ggcaaataac gcagcgggtt tacggatttc cccttgtaac
    39421 gaatttcaaa atgcaagcgt gttgaactgg ttccggtgct acccatggtt gctatttttt
    39481 gccccgcctt cacttcttgt tgttcccgga ccagcattgt gtcgttatgg gcgtaggcac
    39541 tcaggtaatc atcattatgt ttgatgataa tcagattacc gtagccgcgc agcgcgttac
    39601 cggcataaac aacgcggcca tctgcggtcg cgataattgc ctgtcctttg ctgcctgcga
    39661 tatcaatccc cttgttgccc ccctcagaag cgccaaaggt ttcgatcact ttgccctcag
    39721 tcggccagcg ccaggtggag ataggcgtac tggttgatgt actgctgaca gtcggctcgg
    39781 ttgtgcttgc tgttggtacc gttacaggcg ctgtgaccgt ggtcgcagtt ggcttgttgt
    39841 tcggcaacat tttgttagca ctctgttcac ccgaagactc agaatacgta attgtcggtt
    39901 gcgacgcaac agcaacggtg gaattttgtg caggcttgat cacaactcct tgctctgctg
    39961 cgtcggcctg ggtaatggca tttccgccag tgattggcgt accggaagca ttccccacct
    40021 gtaaggtctg accaacgttc agcgcgtatg gtgcctgaat attgttgcgc tgagcaaggt
    40081 cacggaaatc gttgccagta atccaggcga tatagaaaag tgtgtcgcct tttttcacgg
    40141 tgtatgtact gccgctataa ctgcctttcg gaatgttccc atactgacgg ttatagacga
    40201 tgcgtccgtt ttccatctgt accggctgct gagctactgg ctgcactggc tggatttgcg
    40261 gttgttgagt agcctgaatt tgtggctgct gtaccggctg aatttgcggt tgctgcgctg
    40321 tagacgtcgt ccccattttc ggcggcggcg taatcaacat accagaattg gtatttgcag
    40381 gcgcattgcc attgacggaa ctgaccggtg ccggtggatt tgaagtgtca gaacagcctg
    40441 ccagccatag cgaaaccagt gacaaagccg caatgcggcg aacggtgaat tttgggcttc
    40501 ccgcgctcat ttatccccca ggaaaaattg gttaataacc agtgacataa ttaccgtgca
    40561 aggcaccata ctgaacactg gaaaagatgt tcacgatacg ctgacctgcg gcaaaataac
    40621 caggaaaaat ccaggtattt cctcacgttt taagccagct cacccttcac taaagggaca
    40681 aagcgcacgg cctccacggt atcgataata aattcgcctc cccgacgacg cacccgtttc
    40741 aaatactggt gctcctcccc tacgggtaag acgagaatcc cgccttcgtc cagctgcgtc
    40801 attagcgcag ttggaatttc cggcggtgcc gccgtaacaa tgatagcgtc aaacggcgca
    40861 cgtgcctgcc aaccttgcca tccatcgcca tgacgggttg aaacattatg taaatcaaga
    40921 tttttcagac ggcgacgcgc ctgccactgc aaacctttaa tccgttcgac cgagcaaaca
    40981 tgctggacaa gatgcgccag gattgccgtt tgatatcccg aaccggtgcc aatttccagc
    41041 acccgcgact gcggcgtcaa ctcgagtaat tcggtcattc gcgccaccat atacggctgc
    41101 gaaattgtct gcccctgacc tatcggcaaa gcgatattgt cccaggcttt ttgttcaaac
    41161 gcttcatcaa cgaatttttc acgcggcacg gcggcaagtg cattcagcac ctgctcatcc
    41221 tgaatacctt gcgcacgtaa ttgatccaga agtgcttgta cgcgtctgct taccattgcg
    41281 tgccaactcc cacgctgttt aaccagtctg aaaccacatc ttgcgcgcta tgcgcagtta
    41341 aatccacatg cagcggcgtg atggagacat agccctcatc taccgcagca aaatcggtcc
    41401 ccggaccggc atcacattta ccgcccggcg ggccaatcca gtacagcgta ttgccgcgcg
    41461 gatcttgctg cgggatcacc tgttctgccg gatgtcgtgt accgcagcgc gtcacgcgaa
    41521 tacctttgat ttgatccaag ggtaaatccg gaacgttaat attaagaata cgcccggtgc
    41581 gcagcggctc tttacacagt gcgcgcaaaa ttgaacaggt taccgccgcg gcagtgtcgt
    41641 aatgtttatg cccgtcaagc gagacggcaa gcgccggaaa acctaaatga cggccttcca
    41701 tcgcggcggc taccgtaccg gaataaataa catcatcccc cagattcggc ccggcgttaa
    41761 ttccggacac aacaatgtcc gggcgcggac gcatcagagc attcacgcca agatagacgc
    41821 aatcggtcgg ggttcccatt tgcacagcaa tatcaccatt ttcaaaggta aacgtgcgca
    41881 gggaggattc cagtgtcaga gaatttgaag cgccgctgcg gttacgatcg ggggcgacca
    41941 cctgaacgtc agcaaactca cgcaaggctt tcgccagcgt ttgtataccg ggtgcatgta
    42001 ccccgtcatc attactcagc aatatgcgca taatcacctg ttgtgttgat aagttccctg
    42061 acaacgctgg ttgcaaaact acccgccgga agccagaaac ggatctctac ggtgacgtca
    42121 tcccaccagt tccagcttaa ttgttgcgga tacagcagca tcgctctgcg cgcggcttca
    42181 actttttcgc gcaccagtaa ggtttgtaat tcagtttctg cggcgaccgc tgcttgttcg
    42241 aatgccaacg cttcacgctg agttccccat tcgccactgc ccggcaatgc ggcggttatc
    42301 atcagctctt tatcgttgac gcgacgctgt aattccacca gttcttcggt ggttgcaaca
    42361 aaccagctac cacgtccggc taattgtagc gcatcgccgt caacaacttg attaacgtct
    42421 gcttttttga ggcgctcagc aacaatctga ttaaacaacg cactgcgggc tgccgacaac
    42481 caaaaactcc gtttattgcg atcgcgcacc ggagtattgg tttgcgccca gcgcagcgcg
    42541 ccctgcaagt tgctaccgcc aatcccaaaa cgttgggcac cgaagtagtt cggtacacct
    42601 tttacgcaaa tatcgatcag acgttgttca acgtcatcgc gattgctcac ttcgcgcaga
    42661 accagggtaa aggcgttacc tttcagcgcc cctaaacgca gcttgcgctt gtgccgcgca
    42721 tactccagca cctggcagcc ttccaattga aaggcgctca gatcgggcat ttccttgccc
    42781 ggcacgcgag cgcataacca ctgttctgta acagcatgtt tgtctttttg cccagcaaag
    42841 ctgacttcac gggcatgaat tttcaggaat ttcgccagtg catccgccac aaaacgggta
    42901 ttgcagccgt ttttgaggat tctaaccaga atatgctcac cttcaccatc aggctcaaag
    42961 cccaaatctt ccaccaccac aaagtcttcc ggattggctt tcagcagccc ggtgccttgc
    43021 ggtttaccgt ggaggtaagt gagattatca aactcaatca ttttgttgcc ttaatgagta
    43081 gcgccaccgc ttcacaggca atcccttccc cacgtccggt aaatccaagt ttttccgtag
    43141 tagtggcttt cacgttaaca tcatccatat ggcagccgag atcttcggca ataaacacgc
    43201 gcatttgtgg aatgtgcggc aacatcttcg gtgcctgagc tatgatagtg acatcgacgt
    43261 tgccaagggt ataacccttc gcctgaatac gacgccaggc ttcgcgtagc agctcgcggc
    43321 tatcggcacc tttaaatgcc ggatcggtat ccgggaacag tttgccgata tcccccagcg
    43381 ccgccgcgcc aagcaatgca tcggtcaacg catggagcgc cacgtcgcca tcagaatgcg
    43441 ccagcaatcc tttttcgtaa ggaatgcgta cgccaccaat gataattggg ccttcaccgc
    43501 caaaggcgtg tacgtcaaaa ccgtgtccaa ttcgcattat gtattctcct gatggatggt
    43561 tcgggtgagg taaaactcgg ccagtgccaa atcctccggg cgcgtgactt taatgttatc
    43621 cgcacggcct tcgaccaact gaggatggaa tccgcaatat tccagcgccg aggcttcgtc
    43681 ggtaatagtc gcgccttcat ttagagcgcg cgtcagacag tcatgtaaca gctcacgagg
    43741 gaaaaattgc ggcgtcagcg cgtgccataa gccgttgcga tcaacggtat gagcaatggc
    43801 atttttgccc ggttcggcac gtttcatagt atcgcgcact ggtgctgcca ggatccctcc
    43861 cgtgcggctg gtttcgctca acgccaacaa tcgcgcgagg tcatcctgat gcagacaagg
    43921 acgagcggcg tcatgcacca atacccactg cgcgtcgcca gcggctttca aacctgccag
    43981 cacggaatcg gcacgctcat caccgccatc tacaacggtg atttgcggat gattcgccag
    44041 aggaagttgt gcaaaacggc tatcgccagg acttatggca atgacgacac gtttcacccg
    44101 gggatgcgcc agcagcgcat gcaccgagtg ttcaagaatg gtttgattac cgattgagag
    44161 atattgctta ggacattccg tttgcattcg acggccaaat ccggccgccg gaaccacggc
    44221 gcaaacatcc aaatgagtgg ttgccatgtt aattcccggg ctgatttatc gattgttttg
    44281 ccccgcagac tgtgcgcgct tcgacgcgtc aggtaccaga cgataaaaag tttcgcccgg
    44341 cctggtcatg ctgagttcat tacgcgcacg ctcttcgagc gcctcctggc cgccattgag
    44401 atcgtcaatt tcggcaaaaa gttgatcgtt tcgcgcttta agtttcgcgt ttgtagcttg
    44461 ctgtgccgcc acatcatcat tgacgcgggt atagtcatgt ataccgttct taccgaacca
    44521 cagcgaatac tgtagccaga ccagaatagc cagcaacagc agcgttagtt tacccatcct
    44581 gccccctgaa aaacggcatc atcatcccat gcatccgaag acgactctac atcctctgtt
    44641 ggggataccg cgacaacgcg ggcaaatgta ccacatttgt ccattgttac gtatacccag
    44701 ggcgtgcaga acataatctc attattagtt acggtttgaa ttatgaacag aggagacaag
    44761 aaagtacaaa ttagcccagt agccacataa acagtgcgcc aaacataatg cctactgtca
    44821 tcagggtgaa aacaatactg tagcgtagct ttccgtccat caatgaatgc agcgcaatcc
    44881 ccaccactac cgcaacgggc atcagcgcca gaaagaaagg ccaggtgtag ataaagaaga
    44941 acagcgtgtt agagccataa atcaacatcg gcatcgccag cgcaaataac caagagataa
    45001 aaccgaccac ggcaccaggc agtgaccatg tggtttcttc atcctcagta aggctgtcgt
    45061 tatttgttag tgtaatgtta tggctattac gcatatttga tcctgttact ttgacgaacc
    45121 gggcatggaa acccggtggt gtctcaggat ctgataatat cgttctgtct caacagatct
    45181 aataattgct gtaccaaatt tgttactaat tgttcaccat tgagatgaat ttctgccgat
    45241 tcaggcgctt cgtaaacgga atctattccc gtaaagttgc gcagttcacc ggcacgcgct
    45301 ttcttatata agccttttgg atcgcgggct tcgcaaatcg ccagcggcgt atcgacaaac
    45361 acttcgataa agcgcccttc tcctacgcgt tcgcgaacca tctggcgttc ggcgcggtgt
    45421 ggcgagataa atgcggtcag caccaccagt ccggcttcaa ccatcaaatt cgccacttca
    45481 ccgacgcgac ggatattctc tttacgatcg gcatcgctaa aaccgagatc gctgcataat
    45541 ccgtggcgaa cattgtcgcc atccagcaga tacgtactga cgccgagttt atgtaacgcc
    45601 tcctccagcg ccccggcgac cgttgattta ccggacccgg agaggccggt aaaccacagc
    45661 actacaccac gatgaccgtg gtgtagctcg cgttgttgca cagtgaccgg atggctatgc
    45721 cagacgacgt tttcgtcatg cagcgccatt atttatcccc cagcaaatcg cgcgcgcccc
    45781 agtgtggaaa gtggcggcga accagggcat tcaattccag ttcgaatgca ctaaattcag
    45841 atggcgcagc agttgcctgg ctaactggct catgcaccat accggcacct acggtcacat
    45901 tgctcaggcg atcgataaaa atcagcccac cggtaaccgg gttttgctga taacgatcta
    45961 acaccagtgg ctcgtcaaaa gtgagatcca ccaggccgat gccgttcagc ggcaggtttt
    46021 caacttcgcg ttgggtaagg ttattaatat caacctgata acgaatgcca tcaacacgag
    46081 cacgcgtctt cttaccggca attttgatgt cgtaactctg gcccggggaa agcggctgtt
    46141 ccgccatcca taccacatcc accgacgcgc tctgcacagc tggtaacgct tcgtctgccg
    46201 ccagcagcag atcgccacgg ctgatgtcga tctcatccgt cagcaccagg gtgatagctt
    46261 ctccggcaaa ggcttcttcg cgatcaccat caaaagtcac gatccgcgcg acgtttgatt
    46321 ccacaccaga gggcagcact tttacacgtt gcccgacttc cacgcgaccg gatgccagcg
    46381 ttccggcgta gccacgaaaa tcgagatttg ggcggttaac gtactgcacc gggaagcgca
    46441 ttggctgggc atccaccact cgctgaatct ccacggtttc cagcacttcg agcagtgtcg
    46501 gaccgctgta ccacggcata ctttcacttt gcgaagccac gttgtcacct tccagtgcgg
    46561 agagcggcac aaagcggata tccagattac ccggcagctg cccggcaaag gtcagataat
    46621 cttcacgaat acgggtgaac gtctcttcac tgtaatccac cagatccatt ttgttgatcg
    46681 ccacgaccag atgtttgatc cccaacagtg tggagataaa actgtgacga cgggtttgat
    46741 cgagcacgcc tttacgggca tcgatcagta agatcgccag ttcacatgtc gatgcgccag
    46801 tcgccatatt gcgggtgtac tgctcgtgcc ctggagtgtc ggcgataata aatttacgct
    46861 tctcggtaga gaaatagcgg taggccacgt caatggtgat gccctgttca cgctcagctt
    46921 gcaggccgtc caccagcaga gccagatcca gcttttcgcc ctgggtgccg tgacgcttac
    46981 tgtcattatg cagcgatgag agctgatctt catagatttg gcgggtatcg tgcagcagac
    47041 gaccaatcag ggtacttttg ccgtcatcga cgctaccaca ggtcagaaaa cgcagcaggc
    47101 ttttatgttg ttgcgcaatc atccaggctt cgacgccgcc ttcattggcg atttgttgtg
    47161 caagtgcggt gttcatctta aaaatacccc tgacgttttt tcagctccat agaaccagcc
    47221 tggtcgcggt caatcacgcg gccctgacgt tcactggtgg tggaaaccag catctcttcg
    47281 atgatttccg gcagtgtttg tgcatttgac tccaccgcac cggtcagcgg ccagcagccc
    47341 agcgtacgga aacgcaccat ccgttttttg atcacttcgc ccggttgcag gtcgatacgg
    47401 ttgtcatcaa tcatcatcaa cataccgtcg cgttccagaa ccggacgttc cgcagcgaga
    47461 tacagcggca caatgtcgat attttccagc cagatgtatt gccagatatc ctgctcggtc
    47521 cagttagaga gcgggaagac gcggatgctt tcgcctttgt taatttgccc gttgtagttg
    47581 tgccacagct ccgggcgctg atttttcgga tcccagcgat ggaagcggtc acggaaagag
    47641 tagattcgct ctttagcacg ggatttctct tcgtcacggc gcgcaccacc gaaggcggca
    47701 tcaaaaccgt atttattcag cgcctgtttc aggccttcag ttttcataat atcggtatgt
    47761 ttcgcgctgc cgtgcacgaa tggattaatc cccatcgcca cgccttccgg gtttttatgc
    47821 accagcagct cgcagccgta ggctttcgcc gtacgatcgc ggaactcata catctcgcgg
    47881 aatttccagc cggtatcaac atgcagcaac gggaaaggca gcgtacctgg ataaaacgcc
    47941 ttgcgcgcca gatgcagcat gacgctggaa tctttaccga tagagtagag catcaccgga
    48001 tttgaaaatt ctgccgccac ctcgcgaata atgtggatgc tttccgcctc cagttgccgc
    48061 aggtgagtaa gtcgtttttg atccataacc gttcctttgc aataccgcta ttttcttgcc
    48121 atcagatgtt tcgactatag ggagcgtaag agaacgaatg aaattaccaa ttagaatgag
    48181 tagttcctta acggaataac gatttggcaa agctaatatc aaaaagtgct taaggcaccg
    48241 gatttcgggc gtttaggaag atttgaaatt gttttagcgc agcggcagtt tcatactatg
    48301 gcggtaaaaa aatttgcatg gtatttaagg actcactatg ttttccgcat tgcgccaccg
    48361 taccgctgcc ctggcgctcg gcgtatgctt tattctcccc gtacacgcct cgtcacctaa
    48421 acctggcgat tttgctaata ctcaggcacg acatattgct actttctttc cgggacgcat
    48481 gaccggaact cctgcagaaa tgttatctgc cgattatatt cgccaacagt ttcagcaaat
    48541 gggttatcgc agtgatattc ggacatttaa tagtcggtat atttataccg cccgcgataa
    48601 tcgtaagagc tggcataacg tgacgggaag tacggtgatt gccgctcatg aaggcaaagc
    48661 cccgcagcag atcatcatta tggcgcatct ggatacttac gccccgctga gcgatgctga
    48721 cgccgatgcc aatctcggcg ggctgacgtt acaaggaatg gatgataacg ccgcaggttt
    48781 aggtgtcatg ctggaattgg cagaacgcct gaaaaatacg cctaccgagt atggtattcg
    48841 atttgtggcg accagcggcg aagaggaagg gaaattaggc gctgagaatt tactcaagcg
    48901 gatgagtgac accgaaaaga aaaatacgct gctggtgatt aatctcgata acttaattgt
    48961 tggcgataaa ttgtatttca acagcggtgt aaaaacccct gaagcagtaa ggaaattaac
    49021 gcgcgacagg gcgctggcaa ttgcgcgtag tcatggaatt gccgcaacga ccaatccggg
    49081 tttgaataaa aattatccga aaggcactgg gtgttgtaat gacgcagaaa tattcgacaa
    49141 agcgggcatt gctgtacttt cggtggaagc gactaactgg aatcttggga ataaggatgg
    49201 ttatcagcaa cgcgcaaaaa cacctgcctt cccggcggga aatagctggc atgacgtaag
    49261 actggataat caacaacata ttgataaggc acttcctgga agaatagaac gtcgctgccg
    49321 tgatgttatg cggataatgc taccgttggt gaaggagctg gcgaaggcgt cttgatgggt
    49381 tggaaaatgg gagctgggtg ttctaccgca ggggcgggga attctcagtg atatccatca
    49441 tcgcatccag tgcgcccggt ttatccccgc tggcgcgggg aacacatatt tttgtcggtc
    49501 gcctggaagt tcagtttcgg tttatccccg ctggcgcggg gaacacggat tcagaacgcc
    49561 gtcatcctcc gcaatccacg gtttatcccc gctggcgcgg ggaacacaat tcatcatcag
    49621 attcacggtc agcgccaggc ggtttatccc cgctggcgcg gggaacacat ccagcgcgac
    49681 catttttccc tgtaaatttt cggtttatcc ccactggcgc ggggaacacc tatcttgcga
    49741 cagctaaata ttccaacaaa gcggtttatc cccgctggcg cggggaacac aaagtgctga
    49801 aggcgcttaa tgctctcgct ggcggtttat ccccgctggc gcggggaaca ctcatacagc
    49861 agggtgccgt caccgtcacg caacggttta tccccgctgg cgcggggaac acttgcaatc
    49921 tgatggggca gctttcttca atttcggttt atccccgctg gcgcggggaa cacactaagc
    49981 atacatatct gtttttaaac aactttattc cacatcaaca atctaccaac taaattcaaa
    50041 catttcctta tttttaaaga acacataacc tattgattat caacaggaag aaaagaaacc
    50101 aaacgtaacc catccaaatc caccggaata cgtctgtttt ctccccaggt ctgaaattca
    50161 aaacccgact cggtattggt cgcccaggcc atcaccacat ttccgcaacc agccagttgg
    50221 gtaatttgct gccagatcat ctcccgaata cgttttgatg tatcaccaac atacacaccg
    50281 gcacgcactt ccagtagcca gattgcgagc cgtccacgta agcgcggagg gacattttct
    50341 gtaacaacca cgaccatgct catccgccgc gcccccggtg accactatca cccagcgttt
    50401 caggttcagg gatggcaggc ggtaacatat ccggcgcggg ttgtggtggt tcaatttcac
    50461 ctgcagcaag gacttcctca attaacggta ttaatttgcc cgttaactta gtgctacgga
    50521 aaatatcgcg acaggctaat ctgacttctt tatcaggttc tgcgggttgc ctcgctgcta
    50581 tttcaaatgc ctttggcaca accgaatcaa atttaatgat atcggctatg tcataaacaa
    50641 atgaaagcgg tttgccacta tgaatatatc caatagcggg cgcatatccc gcggctaata
    50701 ctgccgcttc agaaataccg tacagacatg atgtggcagc actgatgcag cgattcacaa
    50761 catcgccttt ttcccagtct ttaggatcgt atttgcgacc attccatttc acaccatatt
    50821 gtttcgccag taatgcatag gtctggcgaa cgcgggatcc ctcaattccc cgtagctgat
    50881 ccactgaacg gcgagctggc ggtggctcac gaaaacgtaa ttcatacatt ttgcgcacca
    50941 ccttcaggcg tagatcttcc gttaaagcca gctttgcctg gtagagtaat ttatctgccc
    51001 gcgcccctcc gggttgtccg gaagagtaaa cgcgaacgcc cgcttcaccg acccagacca
    51061 gcagtgttcc caccgtggcg gccagatgca ccgccgcgtg ggaaactctc gttcccggtt
    51121 cgagcataat gcaggcgacc gatcccaccg gaatgtgcgt gcggatcccg gttttgtcga
    51181 tcagcacgaa agcgccgtcc agtacgtcga tttgaccgta ctggaggaag atcatagagg
    51241 tgcgatcttt taacgggatc ggactcagtg gtacaaacgt cacacctctg ctccgggttt
    51301 gatcagcatc agaccacagc cgaacgcgcg gcttttgcca tacccctgac tgagacgttg
    51361 cagaaataac ccgggatcgg tgaccgtaag catccccgta taatccacgc tactgaactg
    51421 gatcagttgc cgggagtttt cccgccgcag ttgctgttgt cggtaggcat caaccgaggt
    51481 atccagcagt gtaaaaccgc ttctctcccc ctgcgctgcc agccagtcca gcgccgcctg
    51541 ttgttgatgc aaccagacat cacgcccttc cgcctgtccc ctcacctgcc gtttcgcctc
    51601 catcagcaga tcgtggcgct tgcccgcttt acaaattgtg ggattcgccc gcaggttgaa
    51661 acaaagggat tgtccggtac gcagctccgg agcaaatgac cggcattcga tagtgaaagt
    51721 ttcactttcc gccgggcgtt cctgcgagag gacaaaaaag cgaaacgcac cctgaagttc
    51781 ctcccgacga taaagaaatt gcctttcttt gccgctaggg aagagatccc acagccactg
    51841 atgcatcaca tattcaccgc gatccaccag atgcagcaac tgcgcaggca aaagctgagc
    51901 ggtatgtaag gttattcttg agaggtacat ggttcctcct tgctgagcca cggcccctga
    51961 ttgacggtgc gttccccaaa cagccactgc tggcgattta aaggaacatc ccggcggcgt
    52021 aatattttgc tcacgaccag gccgtcgtgc tccccttccc accagcattc atcctgaagt
    52081 ttcgggagtg agactttcag gtcgtggaaa tgatcctgat actgttggta tgcgttacgt
    52141 aaaacatcag acgcgttgcc ttcgagcagt aacggcgcaa gtggtaatgc cagcggatga
    52201 ctttttcgcc ccagataaag aggaaaaact ggatggcgta aaccgccctg caactgttcg
    52261 aggctgtaag gcgcatcggc tgttgttgcc accgccacca tccaccaggc gtcggtgtag
    52321 tagtcgcgcc gggagataat cgcgctcaga agatcagggt cgctcaactc ttcgcgacgg
    52381 ctgaaataac gcgctttacg cacctctttt ggcatctgga tcgtgtgata atcccgtgcc
    52441 cagcgcgggt tacggctggc gcaaaccacc agtgaatagt ggcgattaaa cgcgtttaag
    52501 cgttcggtat catcacgccg aatccctacc ccggcggcca gcagacccag caatgctgag
    52561 cgcgaaggca gttcatgggt atgacgcact tcgccggggg catcgacgcc ccaggatgcc
    52621 attggcccat gaagctgaaa aatcagatat tggctcataa gccgcccctt acgcgcagat
    52681 aaagtccagc acgtccttca tgcttccctg tttgttcatc acgtcaaaac ttgcgcattc
    52741 ggtcttctgt tcgtagaccg tattcatatt ttcgcgaagc gttgtaatac gctgcaccgc
    52801 cacgtctaac tgccgggtgc cattaatggg ttcatagaag gccgcagcca gagaacgcgg
    52861 ttgatcggtg cctttttccg ccagcgccca ggaagcatag gcacggctgg caaagctatt
    52921 ttgtttaccg gttggggaaa ctttaagcgc ggcttccgta aaagcgcgta tggtctgatt
    52981 agctaacgct tcgtcaccgc cgaggttttc taccagcaga tctttatcaa tgcagatata
    53041 ggtgtagaaa agtgctgaac cgaatccggt ttctccgaga tgcccggcac cggcatcttc
    53101 agaggcctgg cgcaaatcat caacggcggt gaaaaaatca tcttcgacaa tcgtttcact
    53161 gacaccaaac gcatgcgcga cctggcaggc ggcttcaaca ttaaactcag gtttattcgc
    53221 cagcatacga ccaaacatag cgatatctac cgctatacgg tctttacgta acaacgcgag
    53281 atcttcctct tttggcgcgc gcttttcttc tgccagttga tgggccagcg cttttacggc
    53341 gtcaaattct gccgggctga tatgaactaa ttgttcagtt tcggcgttag tgagcggatc
    53401 ttttggtttt ttgtcgtttt tagctttccc aagataatcc gcaatttttg ccgcccattc
    53461 gatggctttt ttctcttcga ttcctttctc aatcaggata gttgccgcct cacgcgcaat
    53521 tcgcccactg cgaataccaa tatggcccgc cagtgcctgt tcaaaaagtg cagaagtgcg
    53581 ccacgcacgt ttcagacttt gtgaggaaac gcgcagacgc gttgcaccac ccagtaccac
    53641 agtttttggc gcaccagtat catcacggtt aaggttggca gcagggtaag cggttaacaa
    53701 atgaagctga ataaatgttg tcatgagata gtcctttatt aattttgttc gttatcggcg
    53761 tcgccagcct ggtaatattc cagtgcccag cggatacgga taaattcggt tggccgctgc
    53821 tggcggcggt gatgattgag caggtcgtcg ctctcctggc accagcggaa aatatcttct
    53881 gccaaagaga taagattgac ggaaccgttt aataatttga ccgcgcgacg taactgacgc
    53941 agtaaatcat ccggcgtttt tactgccgac aggcgagtaa aacgcccctc cgacattacc
    54001 gccgccagct gcgcggcaaa aggctgccgt tcatcaatgg cttttacatt ggcgctaact
    54061 gcggcgacca gcgccagtgc ggtaaaacgc cactcttgtg cttcaatttt ccacaaagtg
    54121 tgggtctgca tcagcagaga acgaaaccct tctgacaagc aaacatcatt aaccgttgtg
    54181 ctacgccgca gactggcacg attgccgcgt ttagcctgca actcttcgtg ccatttacgc
    54241 agcgtggctt tgtgctcctc ttttacaata ctcattcagc agcctcctgc tttttctccc
    54301 tggcggcttt agcattttgc ttctccgccg atgttgtaaa atatttcttg cgtgcggtca
    54361 tgacgcgttt caaatcaacg ggctcataag gattggtgaa tacacgctcg tcaaaatcct
    54421 gacgcgcaaa taaccaaatt tccttttgcc atttgccgag taattcatct ggatcctgac
    54481 cttcttcaat ttggcgcact aacctcagga agcgatgctg agttttgttc cagaagtcga
    54541 tatccacaaa actgaaatca ccccgtgcac cttttggatc ggagaaccac gcttctttca
    54601 atgcactccg taacagactc agaatccgtg aagccgtttg cgcagccagc cgcagtttcg
    54661 gtatctggcc ttctttttta ttgagcagaa gcgggaaatg gtgctcgtac caacagcgcg
    54721 ctttcatgtt gtcaaaatca taaccaaatc cccacaggcc cacttttgcc tgtttcagac
    54781 tgctggcatt aaagagtttc accaccagcg cgggaagttc cgtattgttt tctgatttcc
    54841 ccgtttcgat aaggcctaac cagtcgcgcc agattaaacc gcccggttgt ggtttaactg
    54901 agtaaaactc gccgccctct ttaagtggta cacggtaagg cgttaaggga tgctgccaca
    54961 tggcatagtt agcgccgtaa tttttggtcg tcatcaggct cagaagcgcg tcgctctgct
    55021 caccgcaaat atcgcagttg ccgactgtgg tggtattaaa atcaatacga atacgccgtg
    55081 gcattcccca gtacgcctgg agtttattaa cctgatcatc ggttaccacc gcaccggcca
    55141 gttcgctggt acgcgtcggg ccaagccagg ggaaaaccag atcgtcaaat ttttggggta
    55201 gcggtaagtc ggcttcatcc tgcggcatca cgttgagcca cagtttgcgc cataagggtg
    55261 tttgttgatt gccctgatat tcctgtaatt caatcagagt ggtcatcggc ccaccgccgc
    55321 gcaaaccggt gcgatagcct ttgccacccg ctggtgcatt caactgtaaa gagaacaagg
    55381 ccaatgctaa acaatggggg cacagatatt ccgttacacc acgcttgata aaatggtctt
    55441 tattaaactt cgtcgtttga gcgccgggaa catcaggcag tagcgaagcg acctgaactt
    55501 tatctcccgt gagcgcctca aaatcctgca taaatgaagg ggaatctggg ccaaactgga
    55561 aagcatgttc taatgacagc aatgcttccc ttagcttttc agcttccagt ccgtcttccc
    55621 atatatcatc ccaacgacga tgatcttttg gcgcaaagct ggtttgtagt agccccagca
    55681 aaaactgcca tgccgcacct tgtaaatctg ctcgcggcgc ggcgatatcg acaacatttt
    55741 catcagccag atcgactggt gccagcttgc ctgtcgttcc gtctttataa cgaacgggca
    55801 accacggggt tgtcagaagt gaaaacgagt tcatacgcat cctctcgtaa aaagaccatc
    55861 cctggtcggt tgtccatcct tccatccttt tcggtgtatc aactattcag agagccctgt
    55921 cgcaggcatc tcaaccaacc ttgccagtca atccttccat agccgaatac ccgcaacttt
    55981 cgtcatcggt gactaaaatc acgtttgcca tctccggctc ttgccgctgt tcaatgcacc
    56041 actgcctgaa cgcatcccct tccagtaaag aaaactcatc ccgatgtttt ttccaccagc
    56101 ttcgacgcac tctgacaacg ctcatttccc atgcgtgagc accggtagca taaggcttca
    56161 ccacaccgtc aatacaggta gccagccaca gggaaacaga ctcttcagcc agacgtgtcg
    56221 acagtttttc cggaaggtaa tcgttgatac tggcggcata gccgggcctg aagttcagca
    56281 caaacttttt agccattgtg cgatcgcagt aatatttgcc cacttgctcc tgctcgctgc
    56341 gggcaaatcc ttccggcatt accacgtcct caccgtaaac tgattcaata agaagccggg
    56401 ctgcgtgtgg catctgaata gcgccttgcc cacgcagcac acgctgcgtc agccagattc
    56461 gcccatgatc ggggtagaca aatgcactgt tccgcatggc tctgccaaac cattcatcac
    56521 ccggagagtc gtcccagacg ggtgccagaa tcagcaattc aggaggggaa cgctcgtctt
    56581 ttccgtcacg ctttaactga ccattaatat cgcgaatatg ccgctgtaat cgccccgctc
    56641 gctgaatcag caaatcaaca ggggccaggt cggagatcat ttcgtccagg tcacaatcaa
    56701 cgctctgctc taagacctga gtacaaatga ggacttttcc ggcacgctgt gaaccgtctt
    56761 ctttaccaaa gcgtgccagc gtctccattt caattcgctg gcgatcgcta aaagcaaagc
    56821 ggctatgaaa gagtgaaagg ctggaagcgg gaatgacgcc gcgggcaagt aactgacgat
    56881 gaactttaat agcgtcatcg acagaattcc ggatccaggc gatgcatttc ccctgactta
    56941 ccgaagattc gatacgcgca atacattctt gttcgctatg gagccaaccc acgctcacgc
    57001 tacgctcaac gtctttgcgc gtcgctaccc ggtgcgaatt cacatccgtt tgcgtgacat
    57061 gcgtcagcca ggggtaatca tctttttcca gcaatggagc ttcttgctgg ccctctgtgc
    57121 cacgcgcaaa ggcggtaaca agtttgtcgc gctgctgttg agataacgtg gcagaaagca
    57181 aaataacgct gtttccacca cgcgcctgtc gctcgatcag cccttcaaga atgcaagaca
    57241 tgtaagcgtc acaggcgtga atctcatcgg ccagcaggat tttgttactc agccccagaa
    57301 gccgcagatt attatgttta aacggcatca ccgccatcat cgcctgatcc agggtgccga
    57361 cgccaatttc agccagcaac gccttcttgt tactgtcggc aaaccaggcc gcacaaccct
    57421 ggctgaaagc ttgttcatcc ggttcttctg acccgattaa atcaccggac cagagtgatt
    57481 cattaaagcg gtccattaat gtgcgggcac tgtgtgccag caccagactg gggcgagatt
    57541 ctggcgaata gaaagaaagc caggttttga cgagccgatc atacatggta ttggccgttg
    57601 ccattgttgg caggccaaaa aacaaaccct gagctttacc cgcagccatc aacctgtgcg
    57661 caagaataag cgccgcttct gttttacctg caccagtcac atcttccaga ataaataact
    57721 gtggcccagg ctggctgata tccagatcca gcaccttttg ctgtaacggt gtcggattct
    57781 caataaaagg aaacagcgta ttaattccgg tgaaaggcgc gacttttgct tttagaggaa
    57841 agacggttaa ggcgttttga gcctgaacta aagttttctg ccagtaacct ttaatatcca
    57901 ttgggtgtgc gacgcgtgga aaaaatcgcg ttgacgaacc tgtccagtct gcgagtacga
    57961 cggttgcaga gatataccag gaaagttgtt ttataagttc aatgccctcg tcatcatccc
    58021 agaatgcggg gatctctatt cgcggaaaca gtgccttgat tgcaaggaga aaatctcgtg
    58081 ctgcagcttt gtcttcaggc agaaaattat ccaattcatc catacgatca gggggccgac
    58141 catgatgccc tgtcgtcacg ggcatccaca tttctatcac tcgcctaagt ttgcgcggag
    58201 agagcgaaga tgaaggaagt aactcctgac attcacttaa ataatgattc cacaaccagt
    58261 agcccagcgt tgaatgagag attttttcgt aattttttct ggcaccttcc tgaatcttga
    58321 gttcaggggg caggtaaagt tgttgaaaag agtgggcaaa ttttccaata tcgtgccagc
    58381 acagcgacca ggcgaaaaat tgagccgcct gttccttgtc agaaatccct aattgataaa
    58441 aataatcagc cagcccgaag caatttcttt taaccattaa atagcccatc gcggccacat
    58501 ccagcgaatg ccagcaaaga aggtgatagt cgtcgccccc ttctttctcg ccgcgtcggg
    58561 tttttcccca gaaatcaaag aaagtcacaa tatttttatc cttcagtaaa cttaaaggat
    58621 atttac
//
LOCUS       DAZFKF010000022        57606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFKF010000022 DAZFKF010000000
VERSION     DAZFKF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.022"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(115..378)
                     /gene="cedA"
                     /locus_tag="ACHKA1_001648"
     CDS             complement(115..378)
                     /gene="cedA"
                     /locus_tag="ACHKA1_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO5679321.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            561..2822
                     /gene="katE"
                     /locus_tag="ACHKA1_001649"
     CDS             561..2822
                     /gene="katE"
                     /locus_tag="ACHKA1_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO5679322.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAIILNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2869..3627)
                     /gene="chbG"
                     /locus_tag="ACHKA1_001650"
     CDS             complement(2869..3627)
                     /gene="chbG"
                     /locus_tag="ACHKA1_001650"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO5679323.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGVVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3640..4992)
                     /gene="celF"
                     /locus_tag="ACHKA1_001651"
                     /gene_synonym="chbF"
     CDS             complement(3640..4992)
                     /gene="celF"
                     /locus_tag="ACHKA1_001651"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO5679324.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5096..5938)
                     /gene="chbR"
                     /locus_tag="ACHKA1_001652"
     CDS             complement(5096..5938)
                     /gene="chbR"
                     /locus_tag="ACHKA1_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO5679325.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5946..6296)
                     /gene="chbA"
                     /locus_tag="ACHKA1_001653"
     CDS             complement(5946..6296)
                     /gene="chbA"
                     /locus_tag="ACHKA1_001653"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO5679326.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6347..7705)
                     /gene="chbC"
                     /locus_tag="ACHKA1_001654"
     CDS             complement(6347..7705)
                     /gene="chbC"
                     /locus_tag="ACHKA1_001654"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO5679327.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7790..8110)
                     /gene="chbB"
                     /locus_tag="ACHKA1_001655"
     CDS             complement(7790..8110)
                     /gene="chbB"
                     /locus_tag="ACHKA1_001655"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO5679328.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8409..8747)
                     /gene="osmE"
                     /locus_tag="ACHKA1_001656"
     CDS             complement(8409..8747)
                     /gene="osmE"
                     /locus_tag="ACHKA1_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO5679329.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            8949..9776
                     /gene="nadE"
                     /locus_tag="ACHKA1_001657"
     CDS             8949..9776
                     /gene="nadE"
                     /locus_tag="ACHKA1_001657"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO5679330.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLRTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10006..10893
                     /gene="cho"
                     /locus_tag="ACHKA1_001658"
     CDS             10006..10893
                     /gene="cho"
                     /locus_tag="ACHKA1_001658"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO5679331.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSQAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(10853..11428)
                     /gene="ves"
                     /locus_tag="ACHKA1_001659"
     CDS             complement(10853..11428)
                     /gene="ves"
                     /locus_tag="ACHKA1_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO5679332.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESTDRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11631..12116)
                     /gene="spy"
                     /locus_tag="ACHKA1_001660"
     CDS             complement(11631..12116)
                     /gene="spy"
                     /locus_tag="ACHKA1_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO5679333.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12446..13414)
                     /gene="astE"
                     /locus_tag="ACHKA1_001661"
     CDS             complement(12446..13414)
                     /gene="astE"
                     /locus_tag="ACHKA1_001661"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO5679334.1"
                     /translation="MDNFLALTLTSKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13407..14750)
                     /gene="astB"
                     /locus_tag="ACHKA1_001662"
     CDS             complement(13407..14750)
                     /gene="astB"
                     /locus_tag="ACHKA1_001662"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO5679335.1"
                     /translation="MNAWEVNFDGLVGLTHNYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREKGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWCY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTQEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(14747..16225)
                     /gene="astD"
                     /locus_tag="ACHKA1_001663"
     CDS             complement(14747..16225)
                     /gene="astD"
                     /locus_tag="ACHKA1_001663"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO5679336.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAVFPRWARLSFAERQAVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPHLLQAGTSLLTPGIIEMTCVAGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFEQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16222..17256)
                     /gene="astA"
                     /locus_tag="ACHKA1_001664"
     CDS             complement(16222..17256)
                     /gene="astA"
                     /locus_tag="ACHKA1_001664"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO5679337.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGIVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17253..18473)
                     /gene="astC"
                     /locus_tag="ACHKA1_001665"
     CDS             complement(17253..18473)
                     /gene="astC"
                     /locus_tag="ACHKA1_001665"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO5679338.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNIINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACERFVSGGSS"
     gene            18919..19725
                     /gene="xthA"
                     /locus_tag="ACHKA1_001666"
     CDS             18919..19725
                     /gene="xthA"
                     /locus_tag="ACHKA1_001666"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO5679339.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            19892..20602
                     /gene="ydjX"
                     /locus_tag="ACHKA1_001667"
     CDS             19892..20602
                     /gene="ydjX"
                     /locus_tag="ACHKA1_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO5679340.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFKAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTAIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            20607..21284
                     /gene="ydjY"
                     /locus_tag="ACHKA1_001668"
     CDS             20607..21284
                     /gene="ydjY"
                     /locus_tag="ACHKA1_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO5679341.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VIFKITE"
     gene            21299..22006
                     /gene="ydjZ"
                     /locus_tag="ACHKA1_001669"
     CDS             21299..22006
                     /gene="ydjZ"
                     /locus_tag="ACHKA1_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO5679342.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            22006..22554
                     /gene="ynjA"
                     /locus_tag="ACHKA1_001670"
     CDS             22006..22554
                     /gene="ynjA"
                     /locus_tag="ACHKA1_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO5679343.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22564..23730
                     /gene="ynjB"
                     /locus_tag="ACHKA1_001671"
     CDS             22564..23730
                     /gene="ynjB"
                     /locus_tag="ACHKA1_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5679344.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIIRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVQEDFSVPTEGAESPWGGAQLTFIARRD
                     VPPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTADPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKSGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            23703..25238
                     /gene="ynjC"
                     /locus_tag="ACHKA1_001672"
     CDS             23703..25238
                     /gene="ynjC"
                     /locus_tag="ACHKA1_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO5679345.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLMIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGNTLASFLPLTGVLCVILLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDRSWTAVVWGHLLWVIPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25238..25891
                     /gene="ynjD"
                     /locus_tag="ACHKA1_001673"
     CDS             25238..25891
                     /gene="ynjD"
                     /locus_tag="ACHKA1_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO5679346.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALPGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDFQDVPADSSVLDMAQWSENYNKLR"
     gene            25958..27265
                     /gene="ynjE"
                     /locus_tag="ACHKA1_001674"
     CDS             25958..27265
                     /gene="ynjE"
                     /locus_tag="ACHKA1_001674"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO5679347.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVNTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TEKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIRPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27274..27894)
                     /gene="ynjF"
                     /locus_tag="ACHKA1_001675"
     CDS             complement(27274..27894)
                     /gene="ynjF"
                     /locus_tag="ACHKA1_001675"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO5679348.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPTCFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            27981..28388
                     /gene="nudG"
                     /locus_tag="ACHKA1_001676"
     CDS             27981..28388
                     /gene="nudG"
                     /locus_tag="ACHKA1_001676"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO5679349.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28354..28626)
                     /gene="ynjH"
                     /locus_tag="ACHKA1_001677"
     CDS             complement(28354..28626)
                     /gene="ynjH"
                     /locus_tag="ACHKA1_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO5679350.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLNTNPLVWQRVKP"
     gene            28862..30205
                     /gene="gdhA"
                     /locus_tag="ACHKA1_001678"
     CDS             28862..30205
                     /gene="gdhA"
                     /locus_tag="ACHKA1_001678"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO5679351.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKSSRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGKQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30322..31362)
                     /gene="ynjI"
                     /locus_tag="ACHKA1_001679"
     CDS             complement(30322..31362)
                     /gene="ynjI"
                     /locus_tag="ACHKA1_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO5679352.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLITMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANHSSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(31489..33450)
                     /gene="topB"
                     /locus_tag="ACHKA1_001680"
     CDS             complement(31489..33450)
                     /gene="topB"
                     /locus_tag="ACHKA1_001680"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO5679353.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRYLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFSPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(33455..34498)
                     /gene="selD"
                     /locus_tag="ACHKA1_001681"
     CDS             complement(33455..34498)
                     /gene="selD"
                     /locus_tag="ACHKA1_001681"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO5679354.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(34615..35166)
                     /gene="ydjA"
                     /locus_tag="ACHKA1_001682"
     CDS             complement(34615..35166)
                     /gene="ydjA"
                     /locus_tag="ACHKA1_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO5679355.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQELSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            35327..37183
                     /gene="sppA"
                     /locus_tag="ACHKA1_001683"
     CDS             35327..37183
                     /gene="sppA"
                     /locus_tag="ACHKA1_001683"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO5679356.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GPIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            37299..38009
                     /locus_tag="ACHKA1_001684"
     CDS             37299..38009
                     /locus_tag="ACHKA1_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFO5679357.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNVPGELFPKLEDAQKKIVAELNAQGINEKEIILGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATDGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            38141..39157
                     /gene="ansA"
                     /locus_tag="ACHKA1_001685"
     CDS             38141..39157
                     /gene="ansA"
                     /locus_tag="ACHKA1_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO5679358.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            39168..39809
                     /gene="pncA"
                     /locus_tag="ACHKA1_001686"
     CDS             39168..39809
                     /gene="pncA"
                     /locus_tag="ACHKA1_001686"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO5679359.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTTLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(39902..41260)
                     /gene="ydjE"
                     /locus_tag="ACHKA1_001687"
     CDS             complement(39902..41260)
                     /gene="ydjE"
                     /locus_tag="ACHKA1_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5679360.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPPLTSYQSNSKVNVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSIFMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     ALGRIVAVFTPYGVAALLTHYGSITVFIVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(41378..42136)
                     /gene="ydjF"
                     /locus_tag="ACHKA1_001688"
     CDS             complement(41378..42136)
                     /gene="ydjF"
                     /locus_tag="ACHKA1_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5679361.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(42273..43253)
                     /gene="ydjG"
                     /locus_tag="ACHKA1_001689"
     CDS             complement(42273..43253)
                     /gene="ydjG"
                     /locus_tag="ACHKA1_001689"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFO5679362.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGALDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(43263..44210)
                     /gene="ydjH"
                     /locus_tag="ACHKA1_001690"
     CDS             complement(43263..44210)
                     /gene="ydjH"
                     /locus_tag="ACHKA1_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5679363.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNYAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(44215..45051)
                     /gene="ydjI"
                     /locus_tag="ACHKA1_001691"
     CDS             complement(44215..45051)
                     /gene="ydjI"
                     /locus_tag="ACHKA1_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO5679364.1"
                     /translation="MLADIRYWENDATNKRYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMARKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(45072..46115)
                     /gene="ydjJ"
                     /locus_tag="ACHKA1_001692"
     CDS             complement(45072..46115)
                     /gene="ydjJ"
                     /locus_tag="ACHKA1_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFO5679365.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(46132..47511)
                     /gene="ydjK"
                     /locus_tag="ACHKA1_001693"
     CDS             complement(46132..47511)
                     /gene="ydjK"
                     /locus_tag="ACHKA1_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5679366.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIHQTGKPLPPVVIADDGKTPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(47538..48614)
                     /gene="ydjL"
                     /locus_tag="ACHKA1_001694"
     CDS             complement(47538..48614)
                     /gene="ydjL"
                     /locus_tag="ACHKA1_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO5679367.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPMCGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(48984..49256)
                     /gene="yeaC"
                     /locus_tag="ACHKA1_001695"
     CDS             complement(48984..49256)
                     /gene="yeaC"
                     /locus_tag="ACHKA1_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO5679368.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(49298..49711)
                     /gene="msrB"
                     /locus_tag="ACHKA1_001696"
     CDS             complement(49298..49711)
                     /gene="msrB"
                     /locus_tag="ACHKA1_001696"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO5679369.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            50053..51048
                     /gene="gapA"
                     /locus_tag="ACHKA1_001697"
     CDS             50053..51048
                     /gene="gapA"
                     /locus_tag="ACHKA1_001697"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO5679370.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            51132..52016
                     /gene="yeaD"
                     /locus_tag="ACHKA1_001698"
     CDS             51132..52016
                     /gene="yeaD"
                     /locus_tag="ACHKA1_001698"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO5679371.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(52067..52921)
                     /gene="yeaE"
                     /locus_tag="ACHKA1_001699"
     CDS             complement(52067..52921)
                     /gene="yeaE"
                     /locus_tag="ACHKA1_001699"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO5679372.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASKCKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLTGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(53011..53757)
                     /gene="mipA"
                     /locus_tag="ACHKA1_001700"
     CDS             complement(53011..53757)
                     /gene="mipA"
                     /locus_tag="ACHKA1_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO5679373.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            54193..56127
                     /gene="yeaG"
                     /locus_tag="ACHKA1_001701"
     CDS             54193..56127
                     /gene="yeaG"
                     /locus_tag="ACHKA1_001701"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO5679374.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            56240..57523
                     /gene="yeaH"
                     /locus_tag="ACHKA1_001702"
     CDS             56240..57523
                     /gene="yeaH"
                     /locus_tag="ACHKA1_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO5679375.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT        14535 a        15005 c        13845 g        14221 t
ORIGIN      
        1 agcaatagat cgtcaaaggc agctttttgt tacaggtggt ttgaatgaat gtagcaatga
       61 aatacaaaat ttcaggccat gtaactcccg gcaaaaccgg gaggtacgta atccttactc
      121 agtcacttcc ccttcctggc ggatctgatt tgcccaacgc tgggcggatt caggcacagt
      181 aaacgccggt gagcgcagaa atgactctcc catcagtaca aacgcaacat atttgccacg
      241 cagcatccag acatcacgaa acgaatccat ctttatcgca tgttctggcg gcgcgggttc
      301 cgtgcgtggg acatagctaa taatctggcg gttttgctgg cggagcggtt tcttcattac
      361 tggcttcact aaacgcatat taaaaatcag aaaaactgta gtttagccga tttagcccct
      421 gtacgtcccg ctttgcgtgt atttcataac accgtttcca gaatagtctc cgaagcgggg
      481 tctggctggt ggtctatagt tagagagttt tttgaccaaa acagcggccc tttcagtaat
      541 aaattaagga gacgagttca atgtcgcaac aaaacgaaaa gaacccacat cagcaccagt
      601 caccactaca cgattccagc gaagcgaaac cggggatgga ctcactggca cctgaggacg
      661 gatctcatcg tccagcggct gaaccaacac cgccaggtgc acaacctacc gccccaggga
      721 gcctgaaagc ccctgatacg cgtaacgaaa aacttaattc tctggaagac gtacgcaaag
      781 gcagtgaaaa ttatgcgctg accactaatc agggtgtgcg catcgccgac gatcaaaact
      841 cactgcgtgc cggtagccgt ggtccaacgc tgctggaaga ttttattctg cgcgagaaaa
      901 tcacccactt tgaccatgag cgcatcccgg aacgtattgt tcatgcacgt ggatcagcag
      961 ctcacggtta tttccagcca tataaaagct taagcgatat caccaaagcg gatttcctct
     1021 cagatccgaa caaaatcacc ccagtatttg ttaggttctc taccgttcag ggtggtgctg
     1081 gctctgccga taccgtgcgt gatatccgtg gctttgccac caaattctat accgaagagg
     1141 gtatttttga ccttgttggc aataacacgc caatcttctt tatccaggat gcgcataaat
     1201 tccccgattt tgttcatgcg gtaaaaccag aaccacactg ggcaattcca caagggcaaa
     1261 gcgcccacga tactttctgg gattatgttt ctctgcaacc tgaaactctg cacaacgtga
     1321 tgtgggcgat gtcggatcgc ggcatccccc gcagttaccg caccatggaa ggcttcggta
     1381 ttcacacctt ccgcctgatt aatgccgaag ggaaggcaac gtttgtacgt ttccactgga
     1441 aaccactggc aggtaaagcc tcactcgttt gggatgaagc acaaaaacta accggacgtg
     1501 acccggactt ccaccgccgc gagttgtggg aagccattga agcaggcgat tttccggaat
     1561 acgaactggg cttccagttg attcctgaag aagatgaatt caagttcgac ttcgatcttc
     1621 tcgatccaac caaacttatc ccggaagaac tggtgcccgt tcagcgtgtc ggcaaaatgg
     1681 tgctcaatcg caacccggat aacttctttg ctgaaaacga acaggcggct ttccatcctg
     1741 ggcatatcgt gccgggactg gacttcacca acgatccgct gttgcaggga cgtttgttct
     1801 cctataccga tacacaaatc agtcgtcttg gtgggccgaa tttccatgag attccgatta
     1861 accgtccgac ctgcccttac cacaacttcc agcgtgatgg catgcatcgt atggggatcg
     1921 acactaaccc ggcgaattac gaaccgaact cgatcaacga taactggccg cgcgaaacac
     1981 cgccgggacc gaaacgcggc ggttttgaat catatcagga acgcgtggaa ggaaataaag
     2041 ttcgcgagcg cagtccatct tttggcgaat attattccca tccgcgtctg ttctggctaa
     2101 gtcagacgcc attcgagcag cgtcatattg tcgatggttt cagttttgag ttaagcaaag
     2161 tcgttcgtcc gtatattcgt gagcgcgttg ttgaccagct ggcgcatatt gatctcactc
     2221 tggcccaggc ggtggcgaaa aatctcggta tcgaactgac tgacgaccag ctgaatatca
     2281 ccccacctcc ggacgtcaac ggtctgaaaa aggatccatc cttaagtttg tacgccattc
     2341 ctgacggtga tgtgaaaggt cgcgtggtag cgattatact taatgatgaa gtgagatcgg
     2401 cagaccttct ggccattctc aaggccctga aggccaaagg cgttcatgcc aaactgctct
     2461 actcccgaat gggtgaagtg actgcggatg acggtacggt gttgcctata gccgctacct
     2521 ttgccggtgc gccttcgctg acggtcgatg cggtcattgt cccttgcggc aatatcgcgg
     2581 atatcgctga caacggcgat gccaactact acctgatgga agcctacaaa caccttaaac
     2641 cgattgcgct ggcgggagac gcgcgcaagt ttaaagcaac aatcaaggtc gctgaccagg
     2701 gtgaagaagg gattgtggaa gctgacagcg ccgacggtag ttttatggat gaactgttaa
     2761 cgctgatggc agcacaccgc gtgtggtcac gcattcctaa gattgacaaa attccggcgt
     2821 aaatatggat aaggtcacgt cccctgggga gtgaccttac caccttactt acacatcaag
     2881 ataactaccc aaccgataac cacgctcagc aattgcatat ttcaacgacg ctgatgtcag
     2941 cacttccagc tccgttaacc gtgggaaaca gtaagcactc tggcgaatcg tattatcgac
     3001 aaacgccgga tggcacatga cttccagcga gcgttccccg cgatggctgg aatcatcaag
     3061 cacttgcagg aacagtgcct cgctaatctc ttcaccataa aacgcgctac tgaatccgtg
     3121 cgaactgcgc agattagccg gtaaatcacc agcgttagat agcggctgac ggtcgatgcg
     3181 caacgcaatc ccctcttcag ccgcaaacct ggcaacaata gggaaaatct gcgggaacat
     3241 atgcacatga tgatggctat caagatgcgt aggtttgcgt ccaaatagct caatgaaacg
     3301 caaatactga ctggcaagct cctgagtaat ttcttccagc ggtaaagcat cttcttctgc
     3361 caactgccag atccattttc ccagcacacc atcgcgggtt aaccccggca tagctgtcag
     3421 tggcttgccc atagtaagga caaagtgcat ccctatggcc agactcggtt catcacgact
     3481 caactgcacc gcatggtcaa tagcctgccc attcaccagc gccgtcgtcg atgtgacaac
     3541 cccattgcga caagcctcga taatgccgta gttctgacct ttgcttaagc caaaatcatc
     3601 ggcattaaca atcagtaagc gttccataat cagcctcggt taatgtgctt ttttaagctc
     3661 tgcgatgcag tcggcaaaat ttggcagcca tttctcgtgc gccagaatca tctcgcgtgc
     3721 cagcagctca gcatcgcgat cggaatgcac caacggacta aggtttagcg ccagtaacac
     3781 atcgttaaat tctccgctaa gtgcggcgtt gctggcagca atctcgaagc ctttaatggt
     3841 gtgaatcagc cccatcactt tatcatcgaa atgcgtaatg cgcggatgtg gcgtcgcgcc
     3901 atcgcgcccc agcgtacagg tcatttctac cgcccagtct gccggaatat tatcaatatg
     3961 cccatgatgc gggatattaa cgtaatgttc tgcttgctta tcgttgtaga tagcgttgat
     4021 cacttcgcac gctgcatcag agtaataagc cccaccgcgc tgttccagtt ctttcggctt
     4081 aactttcaac tccgggtttt tatacagctc aaaaagttgt ttctcgactt tctgtactac
     4141 ctgcgctcgt gcgccgcctt tgtagtattc gcccatttca atagccagca tctctttttg
     4201 cttgaagtaa taaagcaaat aagaacacgg caacagattc agagaacgaa ttaagccttc
     4261 gctaaatggc agatcgaaaa tatttttaac gccagatgct tttaactgcc cggatgccac
     4321 accatcaagt aattcggcaa agcgagattt gccatttacc agcacatcct taatgaacac
     4381 catatggttg aggccgaaca gatcgataga taaatcatcg ctgtctttca gcatcagaac
     4441 atcgcgaata aacatcttca tgccgatcgg aatattacac acgccgataa agcgtttaaa
     4501 tccggtatga cgataaacgg cttcagtgac cattcccgcc gggttagtga agttaatcac
     4561 ccatgcattc ggacaaagtt cttcgacatc ttttacgatg tcaaaaatca ccggaatggt
     4621 acgcagacct ttaaacagac cgcccgcgcc gttggtttcc tgaccaagat aaccatgact
     4681 taatggaata cgttcatcca gttcgcgcgc cggtaattgg cctacgcgca gttgggtagt
     4741 aacgaaatca gcatctttca atgcttcgcg gcgatccagc gttttataaa gcttcatcgg
     4801 gacgccagcg ttatcaatca tccgttggca gagatcgaaa ataatatcca gtttagcttt
     4861 gccaccttcg acatccacca gccataattc gctgaccggc aattcgtgat aacgcttaat
     4921 aaatccttcc agtaactccg gggtatagct gctcccgcca ccaatagtga cgacttttaa
     4981 tttctggctc ataatttctc ccttcagtac agaatactga tatctgacat atctgccccc
     5041 cggacataaa taatccagca acaggacaga tatgtgaatt gtcaggtata acgacttact
     5101 gattaaattc agtcaatttc ttacgatagc tcttaggcgt aaaggacgtt aatttcttaa
     5161 acgttttaat aaacaaactc gggctactat aaccggcctc aaacgcaata tccgtcactg
     5221 aatagttggt catttccagt tgttttttgg caaaattaat acggatttca ttaataatct
     5281 gcattggcgt tttgccataa tatcgttgag tcgctcgcgt caaatattcc tgtgatttgg
     5341 ctgacaacgt caccatattc tccagcgccg attcactaaa ctgctcttta tcatgcatct
     5401 tttctaccgt acttttcagc cactgcggta catcatcaat cacctgttct tcgcggtaat
     5461 gacgtaaacg gttaatgaca taaaaagtaa ccatctcaac aaactcttcc agccctgttt
     5521 cgcggaaatt caatgaagaa atcactgttt ccacataggt gagaaaagcg ttattggtcc
     5581 ggtatacctg cgaagctaca aagcaataag gcaacaacgg caggtaatgc tgctcaaaaa
     5641 agcgtttact gatcccaacg ttcaatatac gcgtggcacc aaactcataa aaactttgat
     5701 ggtgcgaacc taacggaata aaaacaaaat cgccccgttc cagtaacacg cgcttaccgt
     5761 taatctcctg gaaataacgc ccggttaata ccagagtaaa ttcataatag tcgtgctgat
     5821 gcagtccgct gatactctca gttttgttat agataaacac atggaagttt ttgccattaa
     5881 acaactgctg ttctcgggca gtggcaattt ccggcgcgtt aatcactggc tgcatcatcg
     5941 actccttatg ccttcagttt ttcatgaagc tcaattaatt ctgtaatcag ttcacgcgca
     6001 agcatggacg tcattaaatg atcctgagcg tggaccagca ccagactcac tttcatctta
     6061 ccttcgcccg catcgccttc aatcagtttc gtctgtacca gatgcgcttc attcaatgcc
     6121 atccgtgact gatccatcat ggcttttgct gcggcaaaat cgccctgctt cgcctgtttc
     6181 agtgccgcat acgccaggct gcgcgcttgt ccggagttga tgataagccc catcaccact
     6241 tcttccagct cttcagcttc cgtttgcgta tcgggaatat tatcgagatc catcatatat
     6301 cgttcctctc tattcttacc ggcacgatta cccgtaccgg catcgattaa aatttcaggg
     6361 cgttagcgat atcttcttcg ctctcttctt tatcaatcgc gttttgtgct ttgttagcca
     6421 ccacaacaaa gggcagataa attaacgttg cgataccaag gttgaagagt gcaaccagca
     6481 atgcggcgac gctaccgttg gtgttaaaga aggctcccag accggttggc atggtccacg
     6541 gtgcaatatt ggtcaccgga ggaataatgc ccatgtagta cgctgccagg gtgattgccg
     6601 ccagaatcgg ttgtaccagt acaaacggga taaacatcac cgggttcata ataattggca
     6661 gaccaaacag aatcggttcg ttaatctgga agatgccgga cggcagcgcc agttttgcca
     6721 cctgacgata atcagcacgg cgagaggcga taaaaatagc caggatcagg cctaaagtcg
     6781 caccactgcc cccaaggaaa ataaaggagt ccagcatcgg cttggcccag atatggaagg
     6841 tcttaccggc tgccagcgcc gcttcgacgg aaccatattg ctgataggtc gcgatatttt
     6901 ccagcgccca cggcgtcata atgccgttgt ccagtgcggt cagcgccagc gcgccatgaa
     6961 taccgaagaa ccagagcagt ggaacaaaga tcacataggc ccagcccacc acgctaccca
     7021 acgatgccag tggggttgag atggtatcca taatgatctg atggaagttg gtgccccagg
     7081 tattcaacgc ccaggcaata atccccatca cggaaagaat aataaagccg ggaattaatg
     7141 ccgagaagga acgcgatact gaagcaggta cgctgtcggg cagtttaatg acccaattgc
     7201 ggcggacaat aaaggtaaac atttctgcca ccaccaggcc aataataatc ccggagatga
     7261 tattcgcccc acctaaccag tttgcgccaa ccgcataggc ctcaccgaca ctatatgggg
     7321 tgacggtcat aaatgctgca acggataaca acccagccgc cagcgcatcg actttacgct
     7381 cttctgccag cgccatgcca ataaagaacg gtgccattaa agacattatc cctaatgttc
     7441 cgttatatac gttgccgcca atacctttca gaccattaag tgtttcaatg gttgaggcgt
     7501 cgaggcgaat acctaaggaa taaaaaaacg acccctcccc aaagcttaga aaaacgttgt
     7561 taattaatac aaacatggcc cccgcaaggg ttaacggcat taagcgaata aagccatttt
     7621 tgattgcatt aacgtgtggc tgctttccta ttttaactgc aaaagggagg agtacctttt
     7681 caagcgatgc aataacatta ctcatagaaa aataccctta aaaaccgcaa tttaaatatt
     7741 gcggtattga tttatgaaat aactctttga cgggaaaatt taaaataaat taatttgctg
     7801 cggctttttt aatcgctgca accgcagcct taagcacgcc taaaccatcg actttgccat
     7861 aaagcagcga gtcaattact tcaaccggtt tgttgggtaa caaacgctgg atttcgggca
     7921 acatataagc aatctgcggc cctaataaca cgacatcggc attctgacct ttttcaccag
     7981 ccagtgtttc cggaaatgct tcaataatga ccggaacttc atatttttct gcctgtgcgc
     8041 gcatttttga taccagtaaa gaggtagaca tgcccgcaga acaaaacaga taaatgtgtt
     8101 tcttttccat aaaactgccc tcatcgacga ttatctgtca gccagacact ccgcaagcct
     8161 taacctgctt ccatgctctg ggtaacttgc gaaaccaaca tgactttttt tgtggctgaa
     8221 aggagtatac ggtatagacc tgagcaatag tatttctttg accttataaa ttgtctctcc
     8281 ttgacctgcg gttatgaccc tcttcacatt tcgggcaaat aattcgcgaa gataattaag
     8341 aactcagacc aaaaaaaacc ggcgcaatgg ccggtttccg ttgttactca agctttcaga
     8401 cgaagtgatt acttcgcagc ctgtggatca gtgtcgtatt cagcacaggt ctgataaccg
     8461 gagttgatga catgtccggt atcatctaac gcgacaaagt aggtttctgc tttaccatca
     8521 cgttgaccca ggatgtaggt ctggcaagta ccacgagcat ggatcatgct cacttcagac
     8581 gaaggtttac ccgcaatttg tgcaacctgc gcccggctca tgcctttttt gacgtctttc
     8641 accacaggct gtacaaactg gtctttggta cgatcataag ccgtacaacc cgccagcatg
     8701 gttaataccg ccgctgcact cagaattcct gccatattct tgttcatatt ccgtcctctt
     8761 gtttatcagc gtgttagata agcctggaat acattgggcg ctttttcaag cccgtgaacg
     8821 aaacggctcc gctttcagag gattcctgta tgacgtttta accaccattc agcccgctgt
     8881 cgcttgtcgt ttcagtagca acgggttagc tttaaggaag ttttgtcttt tctgtctgga
     8941 ggggttcaat gacattgcaa caacaaataa taaaggcgct gggcgcaaaa ccgcagatta
     9001 atgccgaaga ggaaattcgt cgtagtgtcg attttctgaa aagctacctg cgaacttatc
     9061 cgttcattaa atcactggtg ctcgggatca gcggcggtca ggactccacg cttgccggta
     9121 agctgtgcca gatggcgatt aatgagctgc gccaggaaac cggcaacgaa tcactgcaat
     9181 ttattgccgt acgcctgccc tatggtgttc aggccgacga acaagattgc caggatgcca
     9241 ttgcctttat tcaaccggat cgcgtattaa ccgttaatat caagggcgcg gtattggcta
     9301 gcgagcaggc attgcgggaa gcaggcattg aactgagcga ttttgtccgt ggcaatgaaa
     9361 aagcgcgtga gcggatgaaa gcacaatata gcattgcggg tatgaccagc ggtgtcgtgg
     9421 tgggcaccga tcatgcagca gaagccatta ccggattctt cactaaatat ggtgacggcg
     9481 gtacagacat taatccgctg tatcgtctca acaaacgtca gggtaaacag ttactggcgg
     9541 cattaggttg cccggaacac ctttataaga aagcgccaac ggccgatctg gaagatgatc
     9601 gcccttctct gccagatgaa gtggcactcg gcgtgaccta tgacaatatc gacgactatc
     9661 tggaagggaa aaacgtacct caacaggtcg ccagaacaat agagaactgg tatctgaaaa
     9721 ccgaacataa acgccgtccg ccaattaccg ttttcgatga tttctggaaa aagtaataat
     9781 ttgcacctat tggattgtgc gaaaaagagt aatttgttca cgccggatgc ggcgtgaacg
     9841 ccttattcga cctacaaaac tatgcaaatt caatacattg caggagtcga atgggcctga
     9901 caggcgtagc acgtcagacg gtgtaacctt tgtcatcaac ccgcctcttt tttaatcgct
     9961 tcacgcctgt tacactggat agataaccag ccttcggagt caacagtggt acggcgttta
    10021 acttctccgc ggctcgaatt tgaagctgcg gcaatttatg aatatcccga acatttacgt
    10081 tcattcctta atgacttacc cacccgacca ggggtgtatc tgtttcatgg cgaaagtgac
    10141 accatgccgc tctatatcgg caaaagcgtt aatatccgca gtcgggtgct ttctcattta
    10201 cgcaccccgg atgaagccgc catgctacgg caatcccggc gaattagctg gatctgcacc
    10261 gcaggcgaaa tcggcgctct gctccttgaa gcgcgattaa tcaaagaaca acagccgctg
    10321 tttaataaac ggttgcgccg caatcgccag ctctgtgccc tgcaattaaa tgaaaagcgc
    10381 gtcgatgtgg tgtatgccaa agaggtggat ttttcacaag cccccaacct gtttggcctg
    10441 tttgccaata ggcgcgcagc tttgcaagca ttgcagacca tcgctgatga acaaaaactt
    10501 tgttatggcc tgctgggact ggaaccgtta agtcgcggtc gtgcatgttt tcgttcagcg
    10561 ctaaaacgtt gcgccggagc atgctgcggt aaagagagcc atgaggaaca tgcgctacgc
    10621 ttgcgccagt ctctggagcg tttgcgggtg gtgtgttggc cttggcaagg tgcggtggca
    10681 ctgaaagaac agcacccgga aatgactcaa tatcatatta ttcaaaactg gctgtggctg
    10741 ggggcggtta attcgcttga agaagcgaca acgttaattc ggacacccgc cgggtttgat
    10801 cacgacggtt ataaaattct ttgtaagccg ctgctttccg gtaactatga aattactgaa
    10861 cttgatccgg cgaatgacca gcgagccagt tgatttcact gaacaacagt ttaccttgcg
    10921 gttgtaaaag ccgcagggtg tgcctgccat cgaaccagca cgcgccctgg tcggtagtga
    10981 gcagtttgtc gcccagttgc cacgccccgt taatcacaaa caccaccccg ccgcgtgagc
    11041 caaaggtagt aaacgtacgt tcggcaattc tgactttcgc cttacagaca tccaggcgtg
    11101 tcatgatgtt gaaatccatc gacatctgcc cttccgtcag tttcgctttt accacctggt
    11161 ccgccgcaaa ggcaaaaggt tgtaacggtt ttaaagtatg gttaaagcgg tctgtgcttt
    11221 caagaaacat ctcgccgcct tccagcaacg tcactatcct ttccataccg ggaaacaaag
    11281 aaaactcccc attagccgcg atggatgcaa tgctggcacg ccagtaaaaa tcacgttttg
    11341 ccggtgggaa agtgcaaatt tcgcgcgttt caccggcagc gtttcgccac agattcaccg
    11401 acattttacg catatcaaag tattccatac tcgctcccgg cccgttcttt tcattgttat
    11461 cgttcaggta accgtggatg gttatcggca actcgggtga tttacgttag tggtgatcag
    11521 gcttttttta caactgtttg agaagagaaa agaaaaccgc cgatcctgtc caccgcatta
    11581 ctgcaaggta gtggacaaga ccggcggtct taagtttttt ggctgaaaga ttattcagca
    11641 gttgcaggca ttttaccttt tgccgctgga cgttctgtca gacgcttctc aaaattagca
    11701 ttaaattgct ttttctgttc cggcgtcagg atgttgtaaa ttttgttctg ggtttccatg
    11761 tgcgccagca tgttagcttt gcgctgttct tccatttttg cgatctgcgc ttcagctttt
    11821 gctttatcga aggtatcgct ggcaatgatg tcatgcattg cgcggcgttc ttccagcggc
    11881 ggacgtttca tctggtcacg ctggcctttc atgatttcgc ggatctgctg tttctgcgcg
    11941 tcggtcaggt tcaggtcttt gaacatcatg tcctgatgcg gaccgaactt gcctttgtgg
    12001 tgcatcatcg gcttcgcgtc agccggtgct gcggtagtgg tgtctgcggc atgggccagg
    12061 ttagccgcgc caagagccag ggtagaggca acaaacagtg cagttaattt acgcatattc
    12121 tatatccttc ctttcagtta tttattacgg ctttcttaag tagcgtgccg tgttgacgag
    12181 attaacttta ctgactttag cgtcaattaa tcagagcaac ggtaaaacaa tgaaagtgta
    12241 aaaaacactt ttgcgccaat tatggagaaa aaaagaaaat ttgatggaga gtgatgagcg
    12301 aatattacaa cacgatgatt ttgcagagat tatgaagaac tataccggat gactggtgat
    12361 aaataaagca aataaccagg attaatctgt attaatttat aagaaagcaa cttaataccc
    12421 gcagaatgat ttctgcgggt aagtattagc ttattttttc gagcattaat cccgcgcgta
    12481 accccaacgc taccaacgga ttagggaata acacatactc tacatcatgg gttacggtaa
    12541 aacgttcctc accgtcctgc gccagcaacg ttcctttctt aaacggcata aaattcagcg
    12601 tgtcacttgc catatgcatt tcgaaggacg gcgagtggcg agtaatttgc gaaaccaccc
    12661 gataacggag cggcggtgtt ctcacgatac cgacactctc accagatagc agcgcagcaa
    12721 ttgcgctggc agttactgca aactggcgaa gatcgttttg cccaaagggc aacgctttgc
    12781 caagttccag cgtacaggcc agcgcgccaa aatgtctggc gctgaaatgg gtaaacgtac
    12841 caccaggctc ctgatggaac accagcgcct ccagccccgc cgcacccagc cacgtcagaa
    12901 atttctcgtc ccagggaatg tcgcgttgcg gtaatacacc gaactgcgga tgcaaggagc
    12961 cacgaattgc ggtatgtaga tcaaggtgcc agcgcacaga ttctttgccc tggtcataaa
    13021 aatcttccag gcactgttcc agttcgcgcg cccgacaggt ttctccgctt tcagcaaaca
    13081 gctgccaacg accgccaaac attcggttca tatcgctatg gcaataacgt ttcccttgct
    13141 tcagcgcagg aggattcccg aggatcacca gcaaccgcca acgtaacggg atctcgccgt
    13201 gagatatcgc gccaagcaac gcgtccagca tttccaccgg agccgtttca ttaccgtgga
    13261 tccccgctga aatcaccagt gcgccttgcg gtggcgttaa tggcgtcagt tccagcacac
    13321 catcgcccag ccagcgccag cgaacaccgt tgatttctcg ctcggtgata accggttttt
    13381 tactcgttaa ggtcagagca agaaaattat ccattgcccc ctccctcgcg ctggaacgga
    13441 taaaccgaac cgagattcag taattggctc aatacatcca gtgcttcccg cccttcgcgc
    13501 agcaattgcg ggtcggccag atcggcagca gtaagacgat cgcggtagta acgatccacc
    13561 cagtcattga gcgcattaaa caaggtatcg ttcatcatca ccgccggatt caccgcccgg
    13621 cgttcttctt gtgtcaatac cacccgcaac cgcaggcacg ccgggccacc gccattcgcc
    13681 atgctttcac gtaaatcaaa gacttttagt tcgctgatcg ggttgtcagc ggcaaggagt
    13741 tcattgagat aacaccatac tccggcgtgt tcccgacact cctgtggcag caccaacatc
    13801 atggaaccat catcgcggct caacagttgg ctgttaaaca gatatgtaga caccgcatcc
    13861 gacacggaaa cctgagttgc cggaacttct atcgccataa aaccgttcac ccgcgcacgc
    13921 aggtttgcca gtaactgtga ctggcgagcg aacgcctgtt ggtggcaaaa cagcacctgg
    13981 cggttactca cggcaatcac gtcattatga aaaacgccct ggtcgataac gtccgggttt
    14041 tgctgggcga aaatcacctg ttggggattc acctgattca gccttgccac agcctcgctg
    14101 gcttcgcgag tctgtcgcgc cggataacgg gaaggccggg tatcattgcc tttttctcgc
    14161 ccgtagacaa aaagttgcat acccggttca ccgtaatgac cgccgagacg attgtggttt
    14221 gccgccccct catcaccgag caacgctacc tgcggcaacg ccgaatggac gctaaatttc
    14281 tcttcgtcgt taaaaatagc ttttaacagc gattcagtga cgggcgcttc cagcgaacgg
    14341 tgaaatttat tgttcaggtt ggcaacggtg agatgcactt tgccatccag cgtatcggca
    14401 gatggcgcga tcgttgccgc attggctacc cacattggcg aagcggagct gacgctggaa
    14461 agccagtgcg gtgcctggcg tgcaactttt tccagtacct gctcatcgct accactgaat
    14521 cccaactgac gcagcaccgg aataaacgga cgctcgtgcg gcgggatcac ggcctggggg
    14581 aatcccgcat cggcaagggt tttcattttc agtaagccct gtttcgccgc cagtcgcggg
    14641 ttagacacct gaaaacggtg acgggtagag gcttcattac caaacgacag gccagcgtaa
    14701 ttgtgtgtca gccctaccag cccgtcgaaa ttaacttccc aagcgttcat cgcaccacct
    14761 catcggaaaa atccagtccg gggttaagcg tggcgggtaa cgttaacgag tccgactcca
    14821 ggctcgccat aggccatgcg cagtaatctg cggcatacca ggcgctgggg cgatggttac
    14881 cggatgcacc aatgccgccg aatggcgcgg tactggcagc accagtaagc ggtttgttcc
    14941 agttaacaat ccccgcccgc gcttccagca acagttgttc gaacttttcc cgctcggggg
    15001 aaaccagacc gcaagagagg ccgaagcgag tgttattcgc catcctgatc gcctcatcga
    15061 aattgtcata acgccagacg cgcaagagtg gcccaaacac ttcttcatcc ggcacgccag
    15121 caacgcacgt catttcaatg atccccggcg tcagcaacga tgttccggct tgcagcaagt
    15181 gcggcgcaag cagggttcgt ccgcccatcg cttccagttg ctgccaggca gtaaccacct
    15241 gctgtgcggc ctgttcagaa atcagcccgc caataaacgg ctgcggttcg tcatcccagt
    15301 tgcccggcgt taatcgctgg ctgacggcaa ccagacgagc aagaaacgca tcgccctgcg
    15361 ccccgctttt cagcaataaa cggcgggcgc aggtgcagcg ttgaccggct gtgacaaacg
    15421 ccgactgaat ggtcagatgg acagcggcgt cgatatccgc cacctcatcg ataattagcg
    15481 gattattacc gcccatctca agggcgagaa ttttctccgg ctgaccggag agctggcgat
    15541 gcagctggta gccggtattg gcgctaccgg taaacagcaa gccgtcgaga tcctccagcg
    15601 cactcagcgc ctgacccgtt tcacgcccgc cctgcaccag gttcagcacg cccggcggca
    15661 agccagcctg ctgccataaa cgcattaccg cttcgccact ccacggtgtc agttcgctgg
    15721 gtttaaagat aatggtgtta cctgccagca atgccggaac gatatgtccg ttcggcaaat
    15781 gaccagggaa attatacggc ccaaacaccg ccagcacgcc gtgcgggcga tgtcgcaggc
    15841 tcgccgcgcc gtccggcatt tcactacgct gctcgccggt acgaacgtga tacgccttaa
    15901 ttgaaatcgc gattttattg atcatcgccg tcacttcggt tgccgcttcc cagcgcggct
    15961 tgccggtttc tcgggcaatg attgcggtta attcggcttt attgctttcc agcaatccgg
    16021 caaagcgttc gacaactgcc tgacgttcag caaatgagag ccgcgcccag cgcggaaaca
    16081 ccgcacgggc tgcgcgacaa gcctgcccga cctgagcggc atcggcatca ttgccttgcc
    16141 ataacacctc gcccgatacc ggattacgct tcacacgcga tgcaccctgg cccgttatcc
    16201 agtcaccgtt aatccataaa gtcatgctgt tttctcctct gcgcacaggc gcaccagacg
    16261 aacgcgatcc cctgcgtggc atttgagggc atccagttgt gcggcggtta aaatcaaacg
    16321 ctcggttgcc ggatcggtac gcaccagcac cacgcggaaa tggtgataat tttcattggc
    16381 gaccaggcag gccggaaaat cgccctgcgc aggctgccct tctgccactt ccaccagccg
    16441 acttttacgg atggcgcgca cgcggtcgat gtcacactca agcgtcggcc caccgtcgaa
    16501 gatgtcgata taattacggt agcgaaaacc ttctttctcc agcaccgcgc gggcaggcgc
    16561 ggtttgcgga tgtacctgac cgatgacgtc ctgggcctcc tgggataaaa agtgcgtata
    16621 gatcggatgt ttcggcatca gttctgcaat aaatgccttt tgcccggtgc cgcagagaaa
    16681 atcggcgcgg ctaaaatcca tcgaaaagaa gcgtttaccg aggctttgcc agaacggtga
    16741 atagccgtgt tcgtcaatca ccccgcgcat ttcagcaacc actttgtcat taaacttgtc
    16801 gcgaaaagcc gccataaaca taaagcgcga tttcgacagc aaatagccgt tgccctcttt
    16861 gcgccagtcc gggtcgagaa acagcgtgca cagctcgctg ctgccggtgt gatcgttact
    16921 gagaaacagc gttggcagcg cgttatagac attcagctct ttcgaagcgt gaaccaacgt
    16981 gccgacgcga tagttgtacc agggatcgtt cagcccaact gccacctcaa tggcgcaaat
    17041 ccccgccaca atgcctgtct cgctatcttc cagtacaaac acataaccct gctcactttt
    17101 gggcagttca ccttgccagg ttttgattgc cctttcgata cgcgccgaaa gcgtggcttc
    17161 attggcggga agcgacgtca ggccgccgcc cgttttgctg gcaagctgca tcagcgccga
    17221 gacatctgag cgttcaacgg gacggatgac catcatgatg aacctccgct cacaaagcgt
    17281 tcacaggccg cagcaaagcg atccagtccg gtcgtcacct cttcttcgct gacattgagc
    17341 gcaggcgcaa aacgcaccac gttgccaccc gcaatcagca ccatcacgcc cgctttcgcc
    17401 gcctcctgag agatctgttt cgcttgtccg gcgtaatcgg cattcagtac acagccaatc
    17461 agcaaaccta agccgcgaac ttcactgaac aaaccatagc ggtgattaat aatattaaga
    17521 cgctcaacaa accagtcgtg acgttgctta accccgttaa gcatctctgg cgtattgatg
    17581 agctccagca ctttgcctgc caccgccgag gccagcggat tgccgccata ggtggtgcca
    17641 tgagtgccaa cggtcatcac gctggcgcac tcttcggttg ctaacaacgc accgaccggg
    17701 aaaccgccgc ccagcgcttt ggcggtagtt aacagatcag gcgtcacgcc gtagtgcata
    17761 taggcataca gttccccggt gcgcccgacg ccggtttgta cttcatcaaa aatcaacagc
    17821 gcattgtggc gatcacacaa ttcacgcaga ccttgtaaaa atgcgttgct ggctggcacc
    17881 acaccgcctt cgccctggat gggttcgaca atcaccgcac aggtagagtc gtcaatcagc
    17941 gcgctggcag agttaatatc gttatatgca gcatgacgga tatctggtgg cagtggcgca
    18001 aaatcctgtg aataggctgg ctgcccaccc gcactgacag taaacagcgt gcgaccatga
    18061 aacgcatttt taaacgccac gataccgctc ttatggctgc cgtagcggtc gtgagcgaat
    18121 ttacgcgcca gttttagcgc cgcttcgttg gcttccgcgc cagagttaca aaagaagacg
    18181 cgatcggcaa acgtggcgtc gatcaatttt ttcgccagtc gcagtaccgg ttcgttggtg
    18241 taaccgttgc cggtatgcca gaacttactc gcctgttcgt tcagcgcctc gcgcagttcc
    18301 gggtgcgcat gtcccagcgc gttcaccgca atgccacccg cgaagtcgat atactctttc
    18361 ccctgctgat cccacaagcg cgaaccttcg ccacgtaccg gtataaacgg tgccggagcg
    18421 taaacaggta tcatccattc atcaaagttt tcacgcgtaa ttggctgaga catagcgacc
    18481 tctacagtaa ataattcgtt atttatatgt taataataag taatatttgc gctgtaaatg
    18541 tagattgcag ggttcgtgcc agccagtgat aaaagtgcat aaaccgcgga ggctaactgg
    18601 aaatcaagga gttataacca aaccatatgc atttaaagtg catataaagt gaatacgttt
    18661 gcgatgtggg tgaataaaaa gaataaaaaa cgcaatgtta tgcagaagta aaatataatt
    18721 ctggaattgt gatcattgac gaaatttact ggaaattact gcgccattct gacgcaacgc
    18781 gcaccaaaag cgggcatttt ttgcgccatc gttgacatca ttaacaacca tcgatcaaat
    18841 cacttaacaa caggcggtaa gcaaagcgaa attctgctac catccacgca ctctttatct
    18901 gaataaatgg cagcgactat gaaatttgtc tcttttaata tcaacggcct gcgcgccaga
    18961 cctcaccagc ttgaagccat cgtcgaaaag caccaaccgg atgtgattgg cctgcaggag
    19021 acaaaagttc atgacgatat gtttcccctc gaagaggtgg cgaagctcgg ctacaacgtg
    19081 ttttatcacg ggcagaaagg ccattatggc gtggcgctgc tgaccaaaga gacgccgatt
    19141 gccgtgcgtc gcggctttcc cggtgacgac gaagaggcgc aacggcggat tattatggcg
    19201 gaaatcccct caccgctggg taatgtcacc gtgatcaacg gttacttccc gcagggtgaa
    19261 agccgcgacc atccgataaa attcccggca aaagcgcagt tttatcagaa cctgcaaaac
    19321 tacctggaaa ccgaactcaa gcgtgaaaat ccggtgctga ttatgggcga tatgaatatc
    19381 agtcctggcg acctggatat tggcattggc gaagagaacc gtaagcgctg gctgcgtacc
    19441 ggtaaatgct ctttcctgcc ggaagagcgc gaatggatgg acaggctgat gagctggggg
    19501 ttggtcgata ccttccgcca tgcgaatccg caaacagcag atcgtttctc atggtttgat
    19561 taccgctcaa aaggttttga cgataaccgt ggtctgcgca tcgacctgct gctcgccagc
    19621 caaccgctgg cagaatgttg cgtagaaacc ggcatcgact atgaaatccg cagcatggaa
    19681 aaaccgtccg atcacgcccc cgtctgggcg accttccgcc gctaatttag cagctctcct
    19741 ggctcaaact gggtcaggag aattaacctt gagaaaaatc aacaaactgt cagtaatgat
    19801 ttgttgcctg ccgtcctttg ttataccgtc actgcgtttt tagttgtctg accacttctc
    19861 tattatcaag tttgatatag gaaactccac gatgaacgct gagcgtaaat ttctgtttgc
    19921 ctgtcttatt tttgcgctgg tcatttacgc tatccacgct ttcggtttat tcgatctgct
    19981 caccgattta ccccacttac agacactcat tcgccagagc ggattgttcg gttatagcct
    20041 ctatattctg ttattcatca ttgccaccct ctttctgtta ccaggaagca tactggtgat
    20101 cgcgggagga atagtttttg gcccgctctt agggacacta ctctcattaa ttgccgctac
    20161 gctggcctcc tcgtgctcat tcctgctggc gcgctggatg ggacgtgatt tactgctgaa
    20221 atacgtgggc catagccata ccttcaaggc tatagaaaaa ggcattgcgc gtaacggcat
    20281 cgattttctt attctgaccc gcttaattcc gctgtttccg tacaatattc aaaattacgc
    20341 ttacggatta accgctattg ctttctggcc ctataccctt atttcggcac tcacgaccct
    20401 gcccggaatc gtaatttata ccgtgatggc aagcgatctt gccaatgaag gcattacgct
    20461 gcgctttatt ttacaactct gtctggcggg cctggcgctg tttattctcg tccagctcgc
    20521 caaactctac gcccgacaca aacatgtgga tctgtctgct tcgcgccgca acccacttac
    20581 tcacccaaaa aatgaaggat agaacgatgt tgcaacatta ttcagtgtca tggaaaaaag
    20641 gactgactgc actctgctta ctggctgttg ctgggcttag cggctgcgat caaaaagaga
    20701 atgcggcggc aaaagtggaa tacgacggac tttcgaacag ccaaccgctg cgcgtcgatg
    20761 ccaataacca tacggtaacc atgctggtgc aaattaatgg tcgtttcctc accgacgaca
    20821 ctcgtcacgg tattgtgttt aaagatggct ccaacggaca taaatcgctg tttatgggtt
    20881 atgcgacccc gaaagcattt tatgaagccc tgaaagaggc aggtggtaca ccgggcgaaa
    20941 acatgacgat ggataataaa gaaacgactc atgtcacagg cagcaaactg gatatttcgg
    21001 tcaactggca aggggcggca aaaacgtatt ccttcgatga agtgattgtt gacagtaatg
    21061 gcaagaaact ggacatgcgc tttggcggta atttaacggc agcagaagag aagaaaacgg
    21121 gttgcctggt gtgtctggat agctgcccgg tcggcatcgt cagcaatgca acatacactt
    21181 atggtgcggt tgaaaaacga ggtgaagtta aattcaaagg caatgcctcg gttctcccgg
    21241 cggataacac gctggcaacg gttatcttta aaatcaccga ataaagccag gataaaggat
    21301 gatgaagatg caatcgcgaa aaatctggta ctaccgtata accctcatca tcctgttgtt
    21361 cgctatgctg ctggcatggg cgctgcttcc cggcgtccat gagtttatca atcgcagcgt
    21421 tgcggcgttt gccgccgtgg accaacaggg tatagaacgc tttattcagt cttacggcgc
    21481 actggcggca gttgtctcgt tcttgttgat gattttacag gccattgctg caccgctacc
    21541 tgcgtttttg atcacctttg ccaatgcgtc gctatttggc gcgttctggg gcggcttgct
    21601 gtcgtggacc agttcgatgg ccggcgcggc gctgtgcttt tttatcgcca gagtgatggg
    21661 ccgcgaagtg gtggaaaaat taaccggcaa aaccgtactt gacagtatgg acggcttttt
    21721 cactcgctac ggcaaacaca ctatcctggt ctgtcggtta ttgccttttg tccctttcga
    21781 tccaatcagc tatgctgccg gtttgacttc aatacgtttt cgctcgtttt ttatcgccac
    21841 cgggcttggt caattaccgg cgactattgt ttattcctgg gcgggcagca tgttaacagg
    21901 gggtaccttc tggtttgtca ccggactgtt tattctgttt gccctgaccg tggtgatttt
    21961 tatggcgaag aaaatatggc ttgagcgcca gaagaggaat gcctgatggg tttaccgccg
    22021 cttagcaaaa ttcctttcat tttacgtccg caggcgtggc tgcatcgtcg ccattacggc
    22081 gaggtgctaa gccccattcg ctggtggggg cggatcccgt ttatctttta tctggtgtcg
    22141 atgtttgttg gctggctgga gcgcaaacgc tcaccgctcg atccggtagt acgatcgctt
    22201 gtcagcgcgc gcattgcgca aatgtgcctg tgtgagtttt gtgtggatat caccagtatg
    22261 aaagtcgccg agcgcaccgg cagcagcgat aaactgctgg cagtggctga ctggcggcaa
    22321 agcccgctct ttagcgatga agaacggctg gcgctggagt acgccgaagc cgcaagcgta
    22381 acgccgccaa cggtcgatga tgccctgcgt acccgactgg ctgcgcattt tgacgctcag
    22441 gcgctcaccg aactgacggc attgatcggc ctgcaaaatc tgtcagcccg ttttaattct
    22501 gccatggaca ttcccgctca ggggctgtgc cgtattccgg aaaaacgttc ttaaggagag
    22561 atgatgcgcc attgtgggtg gttgctggga ttgttatcgc tgttttctct ggcaacacat
    22621 gccagtgact ggcaagaaat taaaaatgag gccaaagggc aaactgtctg gtttaacgcc
    22681 tggggcggcg ataccgcaat taaccgctat ctcgactggg ttagcggcga gatgaaaacc
    22741 cattacgcta taaacctgaa gattattcgt ctggcggatg ccgcagacgc ggtgaagcgc
    22801 attcagaccg aagccgcagc cggacgtaaa acgggcggct cggtggatct actctgggtg
    22861 aacggcgaaa acttccgcac cttaaaagag gccaatttac tgcaaaccga ctgggcagag
    22921 actctgccca actggcgcta tgtcgacaca cagctgccgg tgcaggaaga tttttcagtg
    22981 cctacagaag gggctgaatc gccctggggg ggcgcacaac tgacatttat cgcccgccgc
    23041 gatgttccgc cacagccacc acaaacgccg caagccttac tggagtttgc taaagccaat
    23101 cccggcacgg ttacctatcc gcgcccaccg gactttaccg gcacggcgtt tcttgaacag
    23161 ttgctgatta tgctgacggc cgatcccgcc gcattaaaag aagcgccgga cgatgcgact
    23221 ttcgcccgtg tcactgctcc cttgtggcaa tatcttgatg cgctgcatcc gtatttgtgg
    23281 cgcgaaggaa aggatttccc gccttcgccc gcgcggatgg atgctctgct gaaatccggc
    23341 acattgcgcc tgtcgctgac ctttaacccc gcgcatgcgc agcaaaaaat cgccagcggc
    23401 gatttgcctg caagcagtta cagttttggc tttcgcgagg ggatgattgg caacgtgcat
    23461 ttcgtcacca ttcctgccaa cgcgaatgcc agtgctgcgg cgaaggtagt tgccaatttc
    23521 ttgctctcac ccgatgcgca attgcgtaaa gcagatcccg ctgtctgggg cgatccttct
    23581 gttctcgatc cgcaaaaact gcctgatggg cagcgcgaaa cattgcaatc aagaatgccg
    23641 caagatctgc cgccagtact ggctgaaccg cacgcaggtt gggtaaatgc gctggaacaa
    23701 gaatggctac accgttacgg tacgcattaa tctttttgct gtgggcgatg gtggcggtga
    23761 tttatgcacc gctgatcccg gcagctctca cgctgatatc gcctgctttg tcgttgacac
    23821 actggcaggc gttatttgcc gatccacagt taccgcaggc attactggca acgctggtgt
    23881 cgacaaccat cgcggcggtc ggggcgttgt tgattgccct gctgatgatt gtggcgctgt
    23941 ggcctgggcc gaaatggcag cgtatgtgca cccgtctgcc gtggctgctc gccattcccc
    24001 atgtggcttt tgccaccagc gcccttctgc tctttgctga cggagggctg ctttatgact
    24061 atttcccgta tttcactccg ccaatggaca aattgggtat tgggctgggc ttcacgctgg
    24121 cggtgaaaga aagcgcattt ctgctgtgga tcttagcagc ggtattgagt gaaaaacggc
    24181 ttttacagca agtcatcgtg ctggattcgc tgggctacag ccgctggcaa tgcctgaact
    24241 ggctgctgtt gccctccgtc gcgcctgcgc tggcaatggc gatgctggcg attgttgcct
    24301 ggtcgctgtc ggtcgtggat gtggcgatta ttcttgggcc aggtaatcca ccgacgctgg
    24361 cagtaattag ctggcagtgg ttaactcagg gcgacgccga tcaacaaacg aaaggcgcac
    24421 tcgccagcct gctgctgatg ttgttactcg ccgcctacgt tttgctgggc tatctgctat
    24481 ggtgtacctg gcggcgtact attccccgcg tagatggcgt tcgtaagcct gccacgcctt
    24541 tattgccggg caatacgcta gcgagttttt tacccttgac cggtgtgctg tgtgtgattc
    24601 tgctggcgat cctcgcggat cagtcgacga tcaatagtga agcgctcatc aacagcctga
    24661 caatggggct ggtggcgaca ttcatcgctt tgctcctgtt actgctgtgg ctggaatggg
    24721 ggccacagcg tcgccagttg tggctatggt tgcccatttt attaccggca ctgccactgg
    24781 tggcgggcca gtacacgctg gcgctatggc tgaacctgga taggagctgg acggcggtgg
    24841 tctgggggca tctgctgtgg gtaattccgt ggatgctgtt tatcctgcaa cccgcctggc
    24901 agcgcattga ttcacggtta attttgattg cacaaacact gggctggtcg cgggccaaaa
    24961 tcttctttta cgtgaaatgc ccactcatgt tgcgccctac gctgattgcc ttcgcggtgg
    25021 gattttcagt cagtattgcg cagtatatgc cgacactgtg gctgggcgcg gggcgttttc
    25081 cgacgctcac cactgaggcg gtggcattaa gcagcggcgg cagcaacggt attctcgccg
    25141 cccaggcttt atggcaactg ctattaccgc ttattatttt tgccctgacc gcgttagtcg
    25201 caaaatgggt aggttatgtc agacaaggac tccgctaatg ctctgcgtga aaaatgtttc
    25261 gctacgttta ccagaaagtt gcttgctgac aaacgttaac tttacggtgg ataaaggtga
    25321 cattgtcacg ttaatggggc cgtctggctg tggaaaatcc actctgtttt catggatgat
    25381 tggtgcactg cccggacagt tttcttgtac aggtgagcta tggctcaatg agcaacggat
    25441 tgacatgcta cccaccgcac agcgtcagat tggcattctt tttcaggatg cactgttatt
    25501 tgaccagttc agtgtcgggc aaaatttact tctggcgcta ccggcgacac ttaaagggaa
    25561 tgcccgacgt aatgccgtga atgatgcact tgagcgttca ggccttgatg gagctttcca
    25621 tcaggatcct gccactttgt ctggcggtca gcgagcgcgc gttgctctgc ttcgcgccct
    25681 tctcgcccag ccaaaagcgt tactcctgga tgagccattc agccgtcttg atgtggctct
    25741 gcgcgataat tttcgccagt gggtgttcag cgaagttcgc gccctggcga tccccgtcgt
    25801 tcaggtaacg cacgatttcc aggatgttcc tgctgatagt tctgttctgg atatggcgca
    25861 gtggtcagaa aattacaaca aactgcgata acgcaaagtt tttctcaatg cgtcagttca
    25921 gaatggcgca ctcaaaacta caatgtcggg attttcgatg aaacgtgttt ctcaaatgac
    25981 cgcgctggca atggctttag ggctggcttg cgcttcttcg tgggccgctg aactggcgaa
    26041 gcctcttaca cttgaccagc ttcaacaaca aaatggcaaa gcgatagata ctcgccccag
    26101 cgcgttttat aacggctggc cacaaacctt aaatggccct tctggtcatg aacctgccgc
    26161 cttaaacctc tctgccagct ggcttgacaa aatgagcacc gaacagctaa acgcgtggat
    26221 caagcaacat aacctgaaag ccgatgcccc ggtggcgctg tacggtaatg gcaaagatgt
    26281 cgacgccgtc aatacgcgac tgcaaaaagc aggttttacg catatctcca ttctgagtga
    26341 cgcgctaagc gaaccttccc gtctacaaaa actgccgcac tttgagcagc tggtttatcc
    26401 gcaatggctg cacgacctgc aacaaggtaa agaggttacg gagaaacctg ccggagactg
    26461 gaaagtcatt gaagcggcct ggggcgctcc taagctttac cttatcagcc atattcccgg
    26521 tgctgactac atcgatacca acgaagtgga aagcgaaccg ctgtggaaca aagtttctga
    26581 tgaacaacta aaagcgatgc tggcaaaaca cggcattcgc catgacacca cggtcattct
    26641 gtacgggcgt gacgtatacg ctgcagcgcg tgtggcgcag attatgcttt atgccggcgt
    26701 gaaagatgtg cgcctgctgg atggcggctg gcaaacctgg tccgacgcgg gtctgcccgt
    26761 tgagcgcggt acgccaccga aagtgaaagc ggaaccggat ttcggcgtga agatcccggc
    26821 acaaccgcaa ctaatgcttg atatggaaca ggcgcgtgga ctgctgcatc gccaggatgc
    26881 atcgctggtg agcattcgtt cgtggccaga gtttatcggt acgaccagcg gttacagcta
    26941 tattaaacca aaaggtgaaa tagccggagc acgttgggga cacgctggta gcgactcgac
    27001 gcatatggaa gattttcata acccggatgg caccatgcgc agcgccgatg atattacggc
    27061 tatgtggaaa gcatggaata tcagaccaga tcagcaagtt tcattctact gcggcaccgg
    27121 ctggcgcgcg tccgaaacct ttatgtacgc acgagcaatg ggctggaata acgtctccgt
    27181 ttacgacggc ggctggtacg aatggagcag cgatccaaaa aatccggttg caaccggtga
    27241 acgcggcccg gacagcagca aataacattg caattactga cgctggagcg acttcagcgt
    27301 cagataccca ctccacaccc gcgtaaaggt cgtcatccag cacaacgcac caaataccca
    27361 cgcaaaccac ggaaaacagg tcggaaagag gcaactcaga acaaacagca aaatcgtctc
    27421 gctgccttcg gttaacccgc ccagataata aaacgattta tgcgcataac cgggattatc
    27481 aatctgatgt ttcgctgcca gtgcagcaaa ggcgagaaaa ctgctgcccg taccgataaa
    27541 cgcaaacaac aaccagccgc ctgccagcgc attttgctcc ggtgcagcaa gaataaaacc
    27601 aaacggcacc agcgcgtaaa agagaaaatc cagcgagata tctaaaaacc cgcctgcatc
    27661 ggttaatcct ctgcgccgcg ccagtgcgcc atccagacca tcaagcagac gattcaacaa
    27721 aatgacgact agcgccgcca gataccagcc cagagccaga aacggcagag ccagcacgcc
    27781 gatggcaaat cccactaatg ttaaaccatc tggcgtaatg cccggtttat caagaacccg
    27841 cacgcactga tgcaacaacg gtttaatccg gggatgaaga tggcggtcta gcacaggcac
    27901 tccttaaata taaagccttt ctgattgagc aacagtgcgg atattatggc atttttcgct
    27961 tatctgcccg tgtgtaattt atgaaaatga ttgaagttgt tgccgccatc attgaacgtg
    28021 atggcaaaat tttactcgcg caacgccccg cccagagcga tcaggcggga ttatgggagt
    28081 ttgccggtgg taaagtcgag ctggatgaaa gccagcagca ggcgctggtg cgtgagttaa
    28141 acgaagaatt gggcatcgaa gcaactgtgg gtgagtatgt tgccagccat cagcgagaag
    28201 tttcggggcg gattatccat cttcatgcct ggcacgtacc cgacttccac gggacgttac
    28261 aggcacatga acatcaggcg ctggtctggt gctcacctga agaggcgctg caatatccgc
    28321 tggcccctgc tgacattcca ctattagagg catttatggc tttacgcgct gccagaccag
    28381 cggattagtg ttaagggttt tgtcatcacg ctggcattgc agcagtattc cctcggcttt
    28441 aattaccgct ccttcagaat aatttttatc ctgataaaca cagcactgag tacagggctg
    28501 cgctgactgc ccgcctgaac tgaacacttc tggcggtacg tttacctcca cgtccggacg
    28561 ataatgcggg ttagccagtg caattaatgg aaatgctaat actacggcga acaatgctcg
    28621 actcacaggg aactccttaa cgttattgtc tctgctactg ataacggtag ccgggtggca
    28681 aaactttagc gtctgagtta tcgcatttgg ttatgagatt actctcgtta ttaatttgct
    28741 ttcctgggtc atttttttct tgcttaccgt cacattcttg atggtatagt cgaaaactgc
    28801 aaaagcacat gacataaaca acataagcac aatcgtatta atatataagg gttttatatc
    28861 tatggatcag acatattctc tggagtcatt cctcaaccat gtccaaaagc gcgacccgaa
    28921 tcaaaccgag ttcgcgcaag ccgttcgtga agtaatgacc acactctggc cttttcttga
    28981 acaaaatcca aaatatcgcc agatgtcatt actggagcgt ctggttgaac cggagcgcgt
    29041 gatccagttt cgcgtggtat gggttgatga tcgcaaccag gtacaggtca accgtgcatg
    29101 gcgtgtgcag ttcagctctg ccatcggccc gtacaaaggc ggtatgcgct tccatccgtc
    29161 agttaacctc tccattctca aattcctcgg cttcgaacaa accttcaaaa atgccctgac
    29221 tactctgccg atgggcggag gtaaaggcgg cagcgatttc gacccgaaag ggaaaagcga
    29281 aggcgaagtg atgcgtttct gccaggcgct gatgactgaa ctgtatcgcc acctgggcgc
    29341 ggataccgac gttccggcag gtgatatcgg ggttggtggt cgtgaagtcg gctttatggc
    29401 ggggatgatg aaaaagctct ccaacaatac cgcctgcgta ttcaccggta agggtctttc
    29461 atttggcggc agtcttattc gcccggaagc taccggctac ggtctggttt atttcaccga
    29521 agcaatgcta aaacgccacg gtatgggttt tgaaggaatg cgcgtttccg tttctggctc
    29581 cggcaacgtc gcccagtacg ctatcgaaaa agcgatggaa tttggtgctc gtgtgatcac
    29641 tgcgtcagac tccagcggca ctgtagttga tgaaagcgga ttcacgaaag agaaactggc
    29701 acgtcttatc gaaatcaaat ccagccgcga tggtcgagtg gcagattacg ccaaagaatt
    29761 tggtctggtc tatctcgaag gccaacagcc gtggtctgta ccggttgata tcgccctgcc
    29821 ttgcgccacc cagaacgaac tggatgttga cgccgcgcat cagcttatcg ccaatggcgt
    29881 taaagccgtc gccgaagggg caaatatgcc gaccaccatc gaagcgactg aactgttcca
    29941 gcaggcaggc gtactgtttg caccgggtaa agcggctaat gctggcggtg tagcaacgtc
    30001 aggcctggaa atggcacaaa acgctgcgcg cctgggctgg aaagccgaga aagttgacgc
    30061 acgtttgcat cacatcatgc tggatatcca ccatgcctgt gttgagcatg gtggtgaagg
    30121 taagcaaaca aactacgtgc agggcgcgaa cattgccggt tttgtgaagg ttgccgatgc
    30181 gatgctggcg cagggtgtga tttaagttgt aaatgcctga tggcgctacg cttatcaggc
    30241 ctacaaatgg gcacaattca ttgcagttac gatctaatgt aggccgggca agcgcagcgc
    30301 ccccggcaaa atttcaggcg tttatgagta tttaacggat gatgctcccc agggaacatt
    30361 tcttatgggc caacggcatt tcttactgta gtgctcccaa aactgcttat cgtaacgata
    30421 acacgcttca agttcagcat ccgttaactt tctgcgatag cagcagatat gccagtaaag
    30481 aaatcccatt agactatttt tttgataatc ttcttcgctt tcgaacaact cgtgcgcctt
    30541 tcgagaggca agcattatat aatgccaggc cagttcttct tcaattgtcc cgttttgaaa
    30601 agctgtgctt gatatcgaga tcatccatga taattccgcc gcccatatta gcttcgccga
    30661 ggatttaccg gagctatgat tagcgcaatc agagatatag tctgagggaa aaacagcaaa
    30721 tttattcaac aaggcgataa cctgctctgg ggcttcctcc atgtttgctt taaaggtatt
    30781 ggctccatgg tcgccagaaa gaaaatgctc cattaaggca caataacttt cgctatcttc
    30841 gataccccat tgatcctcta aagactcgcg tcttttactt atgatatcga tcgagtcaaa
    30901 aggaagcaca tgatattgga aggtatcttt gccaggttca ggctttcgcg gccagaactc
    30961 cagcgtttca gaccattgct tatgatagaa tcgataaggt gcgatcaatt gtagcgcctg
    31021 taacttctcg atactgagcg gctcaatacc tttagcctga taataatgca gttgttcttt
    31081 ttttgcttta aaaccggccc gaacaataag ccccatcgta attaatagat aaagaaaaga
    31141 gcatcccgcg gtaatcaagc ctctttcatt caaaccgttg gatgttatcg ctgcgaatac
    31201 aaacattaca gcgacaacac atgttaaata aaacccccac ttacaaagca gcatggcctt
    31261 attttcttta atcatccgtt caaaattact attaaatatt tcccagccat taaaagaata
    31321 cttctcgctc ccagggtggt tttgtaataa aacttttttc atcacattcc ctgttattac
    31381 atactttaga ggcgataatt ttatccatgc aaaaaaatat ccaacgaatt ttcttgatcc
    31441 gctggatatt tcagaatatg actcgaatag cacgaaagat tcgctcgctt acgctatcgc
    31501 cccgcttccg gcttcatctg ctggcggact ttttttcgca ctacgtttac gcggtgcagc
    31561 ctttttctta tcagcactgc caccactgcc cggagccaca atgccgcgaa actgccgcac
    31621 cggagtacgt ttggcttgat caataagctg atatagcgtc cccaccagcg gctgcataaa
    31681 gtcctgatag cgacactgct tttcgctgat ttgcgtcagc accgattccc agtgcgcggt
    31741 catgtccggt cgcgtagcca tttctggcag cgaatggaat aacgcttttc cggcgtcggt
    31801 ggagtggata tagcgccctt ttttagtgag gaaaccacgc ttgaacaaca gttcaataat
    31861 cccggcacgc gttgcttctg tccccagacc atcggtcgca cgaaggatct ttttcagatc
    31921 tttgtcctgc acaaagcgcg cgatcccggt catcgccgaa agcagtgttg catcggtaaa
    31981 atggcgaggc ggctgggttt gccgctctac cacttcacct ttttcacaca gcaactcatc
    32041 gcctttcgcc accacaggca atggcgtgcc gtcgttttct tcatcgcgct ctttgctgcc
    32101 taacagcgtg cgccagcctg cttcagcaag aaaacgcgct ttagcgacaa atttgccttt
    32161 ggcaatgtcc agttcgataa cacacttgcg gaacaccgca tccgggctga actgcatcaa
    32221 gtactgacgg gcaatcaggt tatagacctt cgcttcgttc tccgtcaggt tgatcgcaga
    32281 actccgtgcg gtcgggatga tggcgtggtg cgcatcgacc tttttgtcat cccaacagcg
    32341 gttgcgtata tctggatcta ccactggctg cggcaacaga tctggtgcat gaacgctgat
    32401 ggcattcatc accgcgtggc gtccggcaaa atgttcttct ggcaaatagc gacaatcgga
    32461 acgcggataa gtgattagct tgtgtgtttc gtacagcttc tggcagatat caagcacgtt
    32521 ctgcgcactc agaccaaaac gttttgccgc ttcaatctgc aacgccgaaa gcgaaaaagg
    32581 cagcggcgcg gattctgatt cccgtttatc gttatagcta gtgactatag ccggttgacc
    32641 actaatgcgg ttaaccacat gctccgccag tggacgatgt aacaagcgcc cttcttcatc
    32701 ctggtacggt tcacacgctt cgctcggttg ccagatagcg gtaaaccgct catcggcagg
    32761 tgtcacgata tgcgctttga cttcaaagaa gtctttcgcc acgaagtttt caatctcttc
    32821 atcgcggcgc accaccagcc caagcacggg cgtctgcacg cgtcccacgg aaagtacgcc
    32881 ctgataaccg gcattgcgac cgagaatggt atacgcacgg gtcatattga tgccgtacag
    32941 ccagtcggca cgcgctcgcg ccagcgcaga aacgcacagc ggtacaaact cactgttgga
    33001 acgaagacgg tcgatcgccc gctcaaccgc ctgcgggttc aggtcgttta tcaagcaacg
    33061 ctgtacctgc tggcgctttt ccggtgccag ttgtagatag tccagcactt catccaccag
    33121 cagttgccct tcgcgatccg ggtccccggc gtgaacgatt tcgctggctt catgcaggta
    33181 ccgtttgatg acgttaagtt gtttggtcac ggagggtcgg ggctgtaatt gccacttttc
    33241 cgggacaatc ggcaaatccg caagattcca gcgcgcatag cggctgtcgt aggcgtctgg
    33301 ctgcgcctgc tcaagcaggt gaccgataca ccaggtcacc acctgaccat taccgcactc
    33361 gataaagcca tcgcctttcc ggtgcggttt gggcaggaca tcagcaatgg cgcgcgccag
    33421 actcggtttt tcggcaataa acaaccgcat tgaattaacg gatctcaacc atggcacgac
    33481 cgccgcgcgc tggcaccagt tcgccaatcg ccgtcagttc aatgccaaac tcggcggctg
    33541 tagctttaac ctcattttct gcttccggca tgaccgccag cagcaaaccg ccagaagttt
    33601 gcggatcgca cagcaaatcg cgcacttcac gcggcatttc acccatcaga tgaccgtagc
    33661 tggcaaagtt acgttcggtg ccgccaggca ctgcgcccaa cttaatgtac tcttcaaccc
    33721 ccggcaattt tgggatcgcg tcatagtcaa cgcgcgcctg cacacctgcg ccctgacaca
    33781 tttcgctcaa atggcccagc agaccaaaac cagtaacgtc ggtcatcgct tttacgcctt
    33841 cgatgttggc aaaggacgca cctgcgatgt tcatccggca catcacttcc gtcgccagtc
    33901 cctgatgttc tggtttcaac agtgattttt tctcagccgt ggtaagaacg ccgatcccca
    33961 gcggtttcgt caggaacagt ttacatccgg cttgtgcggt actgtttttc ttcacccgct
    34021 cggtcggtac gatccccgtc accgccagac caaaaatcgg ctccggcgca tcgatggagt
    34081 gaccgccagc cagcgcaata cccgcctgac ggcatgcata gcgtccacct tcggttactt
    34141 cgcgggcaat ttctggggaa agtttgttaa tcggccagcc gaggatcgca atcgccataa
    34201 tcggtttgcc ccccatagcg aagatatcgc tgatggcgtt agtcgccgca atgcggccaa
    34261 aatcgaaagg attatcaacg atcggcataa agaagtcggt ggtactgata acgctggtgc
    34321 cattgcccag atcgtacacc gccgcatcgt cgcgggtttc attacccaca agcaaattcg
    34381 gatcaacaaa cttcgcctgc tcactgtgca ggatggtttc caacactttt ggggaaattt
    34441 tacagccgca accagctccg tggctgtatt gggtcaaacg aatcgagttc tcgctcatgg
    34501 acatctcctg tcaatgcaat ccgggtatgg taaccctcat tccgtgaagt gataagtgag
    34561 agtgtctgaa ttcctgcgcc tttgctcaca atccagacag tttcgcgaca attatcagaa
    34621 ataagtgaca aacggcgtcg ggtccgggac gttaatcgac gtagatgctt tcagctgcgg
    34681 cgtaccgagg tagagaaaac cgacaatttt atcctgctca cggcaaccga atgcttcacg
    34741 cactaccgga ctttcagtta atgcgccact gcgccagatg ccgccaaacc cctgggcaac
    34801 ggctgccatt tgcatcgcca tgaccgcgca tccggcagac aattcctgtt cccatcgcgg
    34861 gactttatga ttttcttcgc atttcgccac caccgtgatg atgagcggtg cgcggaacgg
    34921 cgcattacgg gctttgtcga tagctttgtc atcactaccg gcagcaatcg ccccctgttc
    34981 cagtacggcg ctgaaacgct cgcgcccttc cccttcaatc acaaaaaaat gccacggttg
    35041 catggactta tggtccggcg cgcgcatacc cgcacgcagg atgttttgca gttgttcacc
    35101 cgttggtgcg ggttcagcca ggcgggaggc gctacggcga ttgatcaata gttcgagtgc
    35161 atccatttga ttaactcctg tcgtgatatt tattcacaaa attaacacga gagtggattt
    35221 tgttacagca cagtccgcaa ttcctgctga caagtgtcgg ttgggtcatt acgataacca
    35281 catctattgc gcctgtgaca ggtgtgacct taagttggga gaatacatgc gaaccctttg
    35341 gcgatttatt gccggatttt ttaaatggac gtggcgtctg ctgaatttcg tccgtgaaat
    35401 ggtacttaac ctgttcttta tttttctcgt gctggttggt gtggggatct ggatgcaggt
    35461 cagtggtggt gattcgaaag aaacggccag tcgtggtgca ctgctgctgg acatttctgg
    35521 tgtaatcgtc gataaacccg acagttctca gcggtttagt aaattaagcc gccaactact
    35581 tggtgccagt tccgatcgtc tgcaggaaaa ctcactgttt gatatcgtca acaccattcg
    35641 ccaggcgaag gacgaccgca atatcaccgg tatcgtgatg gatctgaaaa acttcgcagg
    35701 cggcgaccag ccgtctatgc agtacatcgg caaagccctg aaagagtttc gtgacagcgg
    35761 gaaaccggtt tatgccgttg gcgagaacta cagccagggg caatattatc tcgccagttt
    35821 cgccaataaa atttggctgt caccgcaagg cgtggtggat ttgcacggtt ttgctaccaa
    35881 cggtctgtac tacaaatcgt tgctggataa gctgaaagtt tccacccatg tgttccgcgt
    35941 gggtacgtat aaatctgccg ttgaaccgtt tattcgtgat gacatgtcac cggcagcccg
    36001 cgaagctgac agccgctgga ttggtgagct gtggcaaaac tatctgaata ctgttgccgc
    36061 taaccggcag atccctgctc agcaggtatt ccctggcgcg caagggttgc ttgagggttt
    36121 aaccaaaacc ggtggcgata ccgcgaaata tgcactggaa aacaagctgg tcgatgcact
    36181 ggcatcgagt gcggaaatcg aaaaagctct gaccaaagag ttcggctgga gtaagactga
    36241 taaaaattat cgcgccatca gttattacga ttacgcattg aaaacgccgg cagataccgg
    36301 tgacagcatc ggtgtcgtct tcgctaatgg cccaattatg gatggcgagg aaactcaggg
    36361 gaatgttggc ggtgatacca ctgcggcaca aatccgcgac gctcgccttg acccgaaagt
    36421 gaaagcgatt gtcctgcgtg ttaatagccc aggcggcagc gttaccgcgt ctgaagtgat
    36481 tcgcgctgaa ctggcagcag cccgggcagc gggtaagcct gtggttgtat cgatgggcgg
    36541 catggcggca tctggtggtt actggatttc tacgccagct aattacattg tggctaaccc
    36601 cagcaccctg accggttcta tcggtatctt cggcgtgatc accaccgtag aaaatagtct
    36661 ggattcgatt ggtgttcata ctgatggtgt ctcaacttca ccgctggcag atgtttctat
    36721 caccagggca ctgccgccgg aagcgcagca gatgatgcag ttaagcattg agaatggcta
    36781 taaacgcttt attacgctgg ttgctgatgc gcgtcattcg acgccggagc agattgataa
    36841 aatcgcccag ggccacgtct ggaccggtca ggacgcaaaa gctaacgggc tggtcgatag
    36901 tctcggggat ttcgatgatg cggtcgccaa agcagcagag ctggcaaaag tgaaacagtg
    36961 gcatctggaa tactacgttg atgaaccgac cttcttcgac aaagtgatgg acaacatgtc
    37021 tggttctgtc cgggcaatgt tgccagatgc gttccaggcc atgttacctg caccgcttgc
    37081 ctcggtagcc tctaccgtta aaagtgaaag tgacaagctg gccgcgttta acgacccaca
    37141 aaaccgttat gcgttttgcc tgacctgcgc caacgtgcgt taaatcctgc aaaaatgtgg
    37201 tcgatatgct gtcggccaca ctcctgaatc ggactgtctt tcactccctc tctccactac
    37261 attcccttgc tcatttagca tctgatatgg agtcatcagt gaacaaggcc ccctctttaa
    37321 ttgccgctat cgtcctcgga ctggggatta gcgcctgtgg ttattttgtt ggcgatggtg
    37381 ttaaacattt aaaaaccaac aaccgttatg tgaatgtgcg cgggctttct gaaaaagaag
    37441 ttcgcgcaga tacggcagaa ttaacaattg cgattaactt taagggtaat gttcccggcg
    37501 aactgttccc gaaactggaa gacgcgcaga aaaaaattgt tgccgaactc aatgctcagg
    37561 ggattaacga gaaagagata atcctgggcc aatggacaag caagcgaaca gattccttct
    37621 atctgaaaga tgatcccaca atgccacgct ataacgcaga cgggtcagtg acgataaaaa
    37681 cccataatgt tgctgctgtc gaaaaagtcg tcgcgaaatt aaacgaactg caagtcgcta
    37741 cggatggcgc tatcgctgag agcaaagtcg cctatcgctt taatggtatt ggcgcactgc
    37801 gcgctgagat gattgctgcc gccaccaaag atgcacgcaa cgccgcacta caatttgcaa
    37861 cggactcagg tagccaggtg gggtcaatta gcgatgcctc acaaggcgta ttccagatct
    37921 ttgccagtgg cagtgatgaa gatgatccta cggcgattaa taaaacggta cgcgtagtca
    37981 cgaccgtaac ctatgcgttg caggattaac cacttttctc cggcgggaca ctaatccctc
    38041 cgtcctgccg gacttttctc atatccacag agtatccagg tgcgctcgct gcccctcacg
    38101 tatatacttt tgctctttcg atatcattca tatcaatatc atgcaaaaga aatcaattta
    38161 cgttgcctac acgggcggga ccatcgggat gcagcgttcc gagcagggtt atataccggt
    38221 gtcaggtcat ctacaacgcc aactggcgct gatgccggaa ttccatcgcc cggagatgcc
    38281 agatttcacc attcatgaat atacgccgct gatggattct tcagatatga cgccggaaga
    38341 ctggcagcat attgctgaag atattaaagc gcactatgac gactatgatg gttttgtcat
    38401 tctgcacggc accgacacga tggcgtatac cgcctctgcg ctgtcgttca tgctcgaaaa
    38461 tctcggtaaa ccggtcattg tgacagggtc acaaatcccg ctggctgagt tacgctctga
    38521 cggacaaatt aatctgctga atgcgttgta cgttgcggcg aattatccga tcaacgaagt
    38581 aacactcttt tttaataacc gactgtatcg cggcaaccgc actaccaaag cccatgccga
    38641 tggttttgat gcgtttgcct ctccaaacct tcctccgtta ctggaagcag gtatccatat
    38701 tcgtcgtttg aatacgccac ccgccccgca cggtgaaggg gaattaatcg ttcatccaat
    38761 caccccacaa ccaattggcg tagtgacgat ttatccaggg atttctgctg acgtcgtgcg
    38821 caattttctg cgccaaccgg tgaaagcatt gattctgcgc tcctatggcg tgggtaatgc
    38881 gccacaaaac aaagccttcc tgcaggaatt acaagaagcc agcgatcgcg gtattgtagt
    38941 ggtcaacctg acacaatgta tgtccggtaa agtgaatatg ggtggttatg ccaccggtaa
    39001 cgccctcgcc catgctggcg ttattggcgg tgcagatatg actgtagaag ccacgctaac
    39061 caaactgcat tacctgctta gccaggaact ggatactgaa accattcgca aggccatgag
    39121 ccaaaacctg cgcggcgaac tgacgccgga tgattaagga gactgcaatg ccccctcgcg
    39181 ccctgttact ggtcgattta caaaatgatt tctgtgctgg tggcgcgctc gccgtgccgg
    39241 aaggtgacag tacggtggat gtcgctaacc gcctgattga ctggtgccag tcgcgcggtg
    39301 aagcggttat cgccagtcag gactggcacc cggcgaatca cggcagtttt gccagtcagc
    39361 acggtgtaga gccttatacg ccaggccaac tcgacggttt gccacaaacc ttctggccag
    39421 atcactgtgt gcagaacagt gaaggcgcac aattacatcc gttactgaac caaaaagcga
    39481 tcgcagcggt gttccataaa ggcgaaaatc ctttagttga cagttacagt gccttttttg
    39541 ataacggccg tcggcagaaa accacgctcg atgactggtt acgcgatcat gaaatcgatg
    39601 aattgatcgt tatgggcctg gctactgact attgcgtgaa gtttaccgtg ctggatgcgt
    39661 tacagttagg ttataaggtg aacgtgatta ccgatggttg tcgtggcgtg aatattcagc
    39721 cacaggacag tgcacacgcg tttatggaga tgtcagcagc tggggcaacg ctatatacgc
    39781 tggcagactg ggaagagacg caggggtaat tttacgctgg cctacagttc tgtactggca
    39841 ttgtaggcca gataaaacac gtcagtggta catctggtaa tggacgccat caacgaaaga
    39901 tttagttcac ctcagaaatc tcttccagcg acaccttccg cgtttcgatg ccaaaaatgg
    39961 agagaaccag cgcgcagagc actaacatca caccaagcac aataaacacc gtgattgacc
    40021 cataatgtgt taataatgcc gcaacgccat agggcgtgaa gactgcgacg atccggccga
    40081 gggcattaac gaaacccgaa ccacgcaggc gtaaatgcgt tggccaaagt tccgggatat
    40141 aaaccgccga cgcaaagcaa acatacatgt ataaaaagaa gatcatcacc agaccataga
    40201 ttaaaatcgc ccactctgta gtctgaattg aatagatata gcctaacaca gcaataataa
    40261 taagtaaggc ggagccaaat aatcgacgag gaaaatgatc aataattaat gccgcaataa
    40321 atattcctac cggagcgcca atcataataa cagcggtcat gaatattgat ttatcgacat
    40381 caatgccgga gttaacaaat atggtcggta tccatacggt aatggtataa agcgaaatgt
    40441 tcattgcaat taaaacagtt atcgcaacta atgtacgtcg taacatttcc cctttaaaca
    40501 ggagccagaa agtacccttg attacgttaa ccttgctgtt gctctgatac gaagtaagcg
    40561 ggggtaaacg aatactcttc tctctttcaa tttgctgctc tacttcacga agttggcttt
    40621 ctgcaccggc gatttgccct ttccctgcca gccatcgtgg cgactcgata aagtatttac
    40681 ctgagagaaa ccaggctaac agtatgccaa taccacccaa cagaaacatt atccgccaac
    40741 taaaaaaagc gataaccacc acacctatcg ccgcagacag catgggcgac cagttaccaa
    40801 caaatgagag ccgcgcggac cattttccac gcaccgtcgc ggggataaac tccgtaaatg
    40861 aggcatagcc aaccataatc agcgccccca ttcctgttcc catcaggaag cgaaagaaga
    40921 tgagccagta catatcaggg acaaaagcgg cccctgttgc agcaataccg acgatgagaa
    40981 gatttatgcg aaacgccctg cgccgcccaa agtaatcacc gatgaaccca ccggtaagag
    41041 agccgatgaa ataaccaaac attaatgccg aggtaaaggc ggcattgagg aaattatttg
    41101 accatccatt gcttaccagc tttgctaaga cgacattacc tgagtaactc aaaaaccccg
    41161 ttaataacag actaaagctt attataccaa atatacgata atgaaaccgg gccaaaggca
    41221 agcggtccag tcttgcgcca atttgatcat attgttccat tgggtgcctc ggatttgcta
    41281 aaaaaggtag caattgcggc aaattcactt gccgcatctt tatcatcatt ttatttccag
    41341 gcacaggaac aaataatatt gacaacgtgt gctaaaatta ccacaccagc tggatattat
    41401 tgtctttgca aaatgctatc cactctgcac ccggtgattt atcagttatt atgtaattaa
    41461 tatgactaaa gtcagctaac tggacaaaag ctttgcgatc aaacttagag tgatcaacca
    41521 ataacgcaac ttctgtcgcc tgacggatca ttgtcttttt gatttcagct tccgcttcat
    41581 tagagtccag cgcgccgctg ttaatatcaa gacctttaca actcattacc atgatatcga
    41641 catgatagcg gctgatgatc tctttagtaa ttcttccttg cagtgaaagt gtatttttgt
    41701 ttagttcccc acccgttgaa acgactttaa tttctgactg agccagaaca tgaatcgctt
    41761 ccgccgagtt ggttagcaac gtcaggtcac tacggtcctg taacaatttg agcaattcca
    41821 ttacggtact actggaatcg gctgccatgg tggttttatt gtcgataaag ggtagtgcct
    41881 tgcgtgcaat aagctgcttc tcttcataaa acgatgaagc gcgcttataa aaatggatat
    41941 tctccgtcaa catcgctgta tttaaaacag caccaccata ggttctggtc aaaaagcctt
    42001 catcttccag cttctcaaga tcgcggcgaa tggtttcttc ggttacctga aaaatcccac
    42061 tcaaatttga gactgtcacc tttttatcgt tggcaaccat ttgcttaatt gcctgaatcc
    42121 tgtcttttgc cgccacgatt acacccctgt atctttttac atcacattag cgcgattatc
    42181 gcataaccga tgtttacttt caaaataacc tgtttgaatc acagattttc atcacagttt
    42241 tcacagaaac agaggtgaat cgtgttgagt atttaacgct ccagggcctc tgccatttcc
    42301 ctcatcaatg ttgcgtctgc atccgataag ttgatattca gtgccgcgac attttcgcgt
    42361 acctgttccg gtgcagtagc cccactaaga atggagatta aatcactctg ttttaatatc
    42421 cacgccagtg ccagagtggg aattgtgcac tgataacgag cacaaagtgg ctgccactgt
    42481 tcaagcatat caatcacttt cagcatgttt tcacgctgga accagacttt atttgcccga
    42541 gcgccgcccg gaacgtaatc acgagtgatg gtgccggtca acaatccctg ctctagcggg
    42601 gaataaacct gaaccacaat gccattatca cgacatagcg gcagcagttc gttttccatt
    42661 gcccggtcga ggatactgta tttcgcctga ataatatcca gttcaccata ttgcagatac
    42721 tcgcggatat ggtcagcatc gacgttagca gcgcctatag cgcgaatttt cccttcagcc
    42781 tttaactcat taagcactgc gacagtttca gcgatcggcg taaaaaatgg cggcaccgac
    42841 tgccagtgcg tcatgtagat atcgatgtaa tcaataccca gacgttgcaa gctggcttct
    42901 acctcttcgc ggatagattc cggggaaagg tttttataca actgccgatc gccaactttg
    42961 ttgaataaac ttccttttcg ttcccagaca atgccgcatt tggtttctac tacaacctgt
    43021 tcacggggca atttttttaa cgcctgaccg acgataactt cactattgcc aaagttatat
    43081 cctggtgcag tatcaatcag gttaatgccg caacgatggg cttcaagaat cgtatcaata
    43141 catatttgcc gatcgagagc gccattccat gcaggaccgc cgccaatggc ccatgtcccc
    43201 aaccccattc gcgaaagcgt aatatccgtt gtgcctaaag gtatcttttt catttgcctt
    43261 cattatcctt cgtattcttc cagcaattgt tcgaccagct ttctgttttt tacgccggtg
    43321 gtggcaccga cgcttagaac cgagatagcc gccgttgcat tggcaaagcg tgcgcattca
    43381 cgcagatttt ttccttctaa cagtgccgca ataaaacctg aagcaaagtt atcgcccgcg
    43441 ccaatggtgt cgatggcggt tattcctgcg accgccggca ccttcatcgt catgtcacca
    43501 cgcttgataa agcagccgtc tttacccgtt ttaatcacca ccgtttttac gccgcacgca
    43561 agaaagcagt cagcaatttc atccagtgtc tctttcccgg tgagtaattt tgcctcggca
    43621 taattaggaa acagataatc gacataactc aatgcttcgc aaatatcatc cagcgtttca
    43681 ttcaaccgcg gtttgatcat atcggcacag ataatcatct gccgggcttt agcttgcgta
    43741 aaaatttctg ttagcgcttt accatccaat agtggactgt tgaaaatact ggccagtgat
    43801 aataatttcg cctgggaaaa ccgcgcaaaa tcaacatcgt caatattcaa tttccacaga
    43861 ctgccgttac ggtttgtgac aaacgtccgc tcgccatcct ccgtcaccag tccaacatta
    43921 atagaggtat ctatgttgac gtcctgcttc aggctttgaa tatcaatatt ctctttgcgg
    43981 caatggtcga gaataaactg ccctgcggca tctttaccaa tacggctcat taatgctgta
    44041 cgatggccca gacgagaaat aattgttgct tcatttattg catcaccccc ggtggtcatt
    44101 gcgattcttt caagaggata agaatccaca tcaaagatat ttttactgac cggttgcaat
    44161 ggaatatcaa caatagcggc acctatacaa ataacgtcga gattatccat atcattattc
    44221 cgctttgcca tcagaaccga acagtttaat tttttccaat gcccgttctt ttacagcttt
    44281 acgtacttca cgttctaaat gcaggaaagg ctgatcctgg ttttctttca ctgcgaccat
    44341 tgcggcctga cagagctccg tatggatgtt gattttcgcg atacccaacg aaattgcagt
    44401 tttaatgtcg gcatcactga tccccgatgc gccatgcaaa accagcggaa cagacacggc
    44461 atcgcgtacg cgtttgacga cctcaaagtt caattgtggc tctgacgtat aaaccccatg
    44521 ctggttgccg atggcgacag ccaatgagtc acagcccgta cgttcaacaa attcagccgc
    44581 ctggtcagga tcggtatagt gatagcccgc cagcgcttcc tcataaacgg tttcattacc
    44641 gacatgccct aattccgcct ctaccggaat acccagcgga tggaagaaat cgacagcctc
    44701 tttggttaag cgaatatttt cttcgaaatc aaatgcggaa gcatcacgca ttaatgaatt
    44761 cataccatga gtccaggcgt tatgaataat ctccatactc cgaccatgat cccaatgagt
    44821 tattaccggc accgttgctt ttcgtgccat tgataccatc atgtgagaga aatcttcaaa
    44881 tgaggtgttg ccgacaaaac ctgtaccaaa agaaataata accggtgatt tcgcttcttc
    44941 ggctgcgtcg ataacgccca tcaacatttc tgcattccat acattaaaat gggcaatagc
    45001 ataacgttta tttgtggcat cgttttccca atatctgata tctgcgagca tgttctgttc
    45061 tcccgtatta attaatcgct aatcttaata acgcctttaa taatgtcgcg tttattgtta
    45121 actgactctt caaatgcctg ttgtacatcc cgataatcgt aaatatgcgt caccatcgat
    45181 ttcacatcga atcgccctga agaaatagct tcaatcgtga ccggataacg attggcgtag
    45241 cggaataccg tctggatagt aacttcgcga tttattttga ggaaattgat tgccgaatcg
    45301 ccgggtacag taccaacaat cataatttta ccgccgcgca ttaccagata aggtgcctgt
    45361 ttaacggtga ccgcagaacc cgctgtttcg aaaacaatat ctgcgcccat gtcttcggta
    45421 aattgctgac agcgtgcaat agtgtcttct tttgcgccgt taataaccac tgtcgcacca
    45481 agctgttccg ccattgccag acgtttttcc agcacatcaa cgacggcaat ttcagttgct
    45541 cccaggcatt tgcacgcttg caacgtcatc aaaccaatac aacctgctcc cagaataatt
    45601 atcttcttac ccggtttaac atctgccagc atcgcggcat gcatcccgac tgcggcaggc
    45661 tccaccagcg ccccttccat cgtgtccata ttgtcgggca gtttgtaagt aaagctctcc
    45721 ggatgacaca gatagtgtgt taatgcgccg cggtagttgg gttgtgtcgc cataaaatca
    45781 acgtccgggc agatgttata tttgccttcc agacagtaac gacagtgacc gcaaggaacg
    45841 ccaggttcga tatttacccg atcccccggt ttaaatttgc gcacgcggct tcccacagcc
    45901 acaaccgtcc cggcgcattc atgacccagg ccaatttctt gatttgggtc tttaggcgga
    45961 ataaacggac ctgattcaaa accatgtaca tctgaaccac aaataccgac atattctact
    46021 ttaatcaaaa cttcatcttc tttaggcact ggtatttctg ctgaaataat tttcattgtg
    46081 cccggcacct gcaatattgc ttttgaattt ttcattttga atccacccgt attatttatt
    46141 ggctactgca tcaatactta aactttcaac ggagacacct ttggtttcga ttccaatggt
    46201 ggcgatagca attgcgacaa taattgaaac cgcccccaga agaataaaga ctcccgtaac
    46261 gccataacta ctgagcagca ctgcaacggc ataaggtgcg gcaataccac tgattcgccc
    46321 taccgcattc gccagaccgg agccacggag tttggcctct gtcggccaga tctcagggac
    46381 atacactgcc gaggcatagc aaacgtacat atagacaaaa gtaatcagga agaaaccaat
    46441 caacgttatg agcaacatac tggtttgcag tgaatagata tatccgagca ccgcaatcag
    46501 gattaatagc cccacaccca ttgttttacg cggaatttta tccatcacca gcatggcaat
    46561 aaaaatgcca aatggcgcgc caaacatact catggtattt aaaacaatcg agtcttttaa
    46621 attaatcccc tgggtcatga atatcgttgg caaccagtta attagtgtgt actgcacaac
    46681 gttcatggca atcagcacac aagagcctaa tatcacgcgt ttcagtaata ctcctgtcag
    46741 taaggctgaa tacggcaccg cttgtggcgt tttaccgtca tcagcaataa ccacgggcgg
    46801 caaaggtttg cccgtctggt gtatgacgcc ttcttctata ctccgcatga ctttctcggc
    46861 ttcctgatac cgtccgcgcg attccagcca gcgcggggat tcaggaaagt agcgccaggc
    46921 cagcgccgta gcgataagcg acaatattgc agggataagc agttgtaccc gccagttcca
    46981 ctctgcacta atcagcggcg tgagtcccat cgctatcaat gaacagagcg gatatgacca
    47041 gttgccaata aaggaaaccc gacttgacca cgttccacga tttctaccgg gcatatattc
    47101 ggtgaaacca gcaaacagcg taaccagtaa agctcccagc ccaactccca tcacaaaacg
    47161 gcaggcaata aggaaatcca tattcggtga gaaagcaccg acaaccatcg aggcaatatg
    47221 aatggcctca tagaggatga acgcatttct gcgaccggtt ttgtcgccaa tgatgccgcc
    47281 caccagcgca ccgagaaaca ttccggcggt cgtgattgct gagaatgtgg ctgtggtgga
    47341 attatctgtc cagcccaacg ctttcagctg cgcgaggatc aagccaccaa cggcattact
    47401 ccagcagaca agcaagccaa acgcgacgat ggcaaacatt gacgaatgcc agcggcaatc
    47461 cggtaagcga tccagccgtg caccacaatg cggttttgtt atctgttcca tgcgataacg
    47521 ttccttcagg aaagttgtta ttcatcaaag tcgtaagtca tgatcacttt gattgcggtt
    47581 ttatcgacca tcgcatcaaa cccttcgcgc cattgcgaca ggccgatacg atgcgtgatc
    47641 atcggtttga ctttgatagc gccgctggct aatagcctga tagcgttacg ccatgaggtg
    47701 gagtcatagg ccatatgccc aatgatgctt ttgttccagg cggtaatgtc attaatcgag
    47761 aaatcaagag gtttgaagcc cattccaacg cgtaccactt ccccgttcgg gcggagcatt
    47821 tcgatggctt gtttcagtgc gatattggca ccggagcatt caatcaccag tcccagattg
    47881 tcttttccac aaatttgctg gcagcgcgcc accacatctt cggtagaacc atttactact
    47941 gccgtcgcac ccagttcttt tgcaaccggg aagcggaccg ccacatcttc ttgcagacca
    48001 acgacgacga tatttaccgc ccccataatt cgcgccattt gtacggagaa cagcccgagt
    48061 gggccagtgc cgatgacgac cacatcctga ccaggaagga atttcgattg ctgcgcgatg
    48121 gatttgtagg cattacagat agggtcaagt acggctgcgt cctcataatc aacaccatca
    48181 gggatttccc acaacgcatg acgatgaatt ttgagaattt caccaggaac cagacaatat
    48241 ttggaaaaac caccgcccca ggtattatta tccagaccaa ggtttacttt ttctgtacaa
    48301 cacagaaaat caccttgttc acaggccgga caaacgccgc aaacgtgacc gctgttatcc
    48361 gatacgacgc gttgccccac tttccagtct ttgacttttt caccaacctg cgcaatacaa
    48421 cctgcgaact catggccgcg gatagagtta aactcatcag aaccgctatc gacattgtag
    48481 tgcttcatgt ctgcgccgca gattgccgcg gctttaattt caatcactac atcttccggg
    48541 ccacacatgg gttgtgggac atcaatcatc ttgtagccgc caaaggcttt gccaaaccga
    48601 gccagtgctt tcattgcgtc atcctctgtg ttttgttttg cttagatggt catcatactt
    48661 acccacatga tttttgaggt caacattaaa tctgtaaaac acagataatg atctgcgttt
    48721 tacaactcag atcacaatta cgcagaaaat ggttggttct gttacaggtt gatggaaggc
    48781 tgaacgcaaa aggagcaaat ttgagaggga ttaccggcca aataggtctg agacttaagt
    48841 tcaggagaag tcattcaggc gtgaacgcga cggcaagaga ccgggctggg gctttgcctg
    48901 gtcgtgacga gataattgag atgagtcgtg gggttattgt tggctatccc ctttataacc
    48961 gcggcaaaaa ggggacaaac cacttactta aacatggcaa taggctttgc tgagatacca
    49021 aaatcttctt ttaactgttg tttgctcttc atgaccatct gaccatttgt gtcaattgtc
    49081 atatgctgtg cttcggtatt attgcgggct tgccacagca tcaccagttg caggcagttc
    49141 tctttttgtt cctcggtcaa cgcaacgcca tcaggccatt tccccagttc aacggcggtc
    49201 gacaaacgct ggtatacctc aggcgtcatg ctgttgataa tgtcatcaag attcataatt
    49261 tttccgctcc tgtggaataa gttgctgaat cgttttttca accattgatt tcttcgccgt
    49321 tttcgccatc agtgaagcgt aaagaagcag agttaacaca ataacgttcg cccgttggct
    49381 gcggcccgtc ggggaagaca tgccccagat gggcatcaca gttaccgcaa cgaatttcta
    49441 tgcgctgcat tccatgtgac aagtctttga tataacgaat ggattcttca ctcaccggtt
    49501 cgtagaaact gggccagcca cagccggaat catacttggt ttgggaatga aacagcgggg
    49561 catcgcagat caaacagtga tatacgccgt cacgcttgtt atgcagtaaa cgacccgtaa
    49621 atggcggctc tgtcccatga ttctgcgtca cgtaaaactg catctcggac aaattttttt
    49681 tcagttcttc tgccgaaggt ttattagcca tttgctcaca tctcacttta atcgtgctca
    49741 cattacgtga ctgattctaa caaaacatta acaccaactg gcaaaatttt gtcctaaact
    49801 tgatctcgac gaaatggctg cacctaaatc gtgatgaaaa tcacattttt atcgtaattg
    49861 ccctttaaaa ttcggggcgc cgaccccatg tggtctcaag cccaaaggaa gagtgaggcg
    49921 agtcagtcgc gtaatgctta ggcacaggat tgatttgtcg caatgattga cacgattccg
    49981 cttgacgctg cgtaaggttt ttgtaatttt acaggcaacc ttttattcac taacaaatag
    50041 ctggtggaat atatgactat caaagtaggt atcaacggtt ttggccgtat cggtcgcatt
    50101 gttttccgtg ctgctcagaa acgttctgac atcgagatcg ttgcaatcaa cgacctgtta
    50161 gacgctgatt acatggcata catgctgaaa tatgactcca ctcacggccg tttcgacggt
    50221 accgttgaag tgaaagacgg tcatctgatc gttaacggta aaaaaatccg tgttaccgct
    50281 gaacgtgatc cggctaacct gaaatgggac gaagttggtg ttgacgttgt cgctgaagca
    50341 actggtctgt tcctgactga cgaaactgct cgtaaacaca tcaccgctgg tgcgaagaaa
    50401 gtggttatga ctggtccgtc taaagacaac actccgatgt tcgttaaagg cgctaacttc
    50461 gacaaatatg ctggccagga catcgtttcc aacgcttcct gcaccaccaa ctgcctggct
    50521 ccgctggcta aagttatcaa cgataacttc ggcatcatcg aaggtctgat gaccaccgtt
    50581 cacgctacta ccgctactca gaaaaccgtt gatggcccgt ctcacaaaga ctggcgcggc
    50641 ggccgcggcg cttcccagaa catcatcccg tcctctaccg gtgctgctaa agctgtaggt
    50701 aaagtactgc cagaactgaa tggcaaactg actggtatgg cgttccgcgt tccgaccccg
    50761 aacgtatccg tggttgacct gaccgttcgt ctggaaaaag ctgcaactta cgagcagatc
    50821 aaagctgccg ttaaagctgc tgctgaaggc gaaatgaaag gcgttctggg ctacaccgaa
    50881 gatgacgtag tatctaccga tttcaacggc gaagtttgca cttccgtgtt cgatgctaaa
    50941 gctggtatcg ctctgaacga caacttcgtg aaactggtat cctggtacga caacgaaacc
    51001 ggttactcca acaaagttct ggacctgatc gctcacatct ctaaataagt tgagatgaca
    51061 ctgtgatcta aaaagagcga cttcggtcgc tctttttttt acctgataaa atgaagttaa
    51121 aggactgcgt catgattaag aaaatttttg cccttccggt catcgaacaa atctcccctg
    51181 tcctctcccg tcgtaaactg gatgaactgg acctcattgt ggtcgatcat ccccaggtaa
    51241 aagcctcttt tgcattacag ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag
    51301 aagttctgtg gttgagcaac aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg
    51361 taccggtttg ctggccgtgg tttggtccgg cggcacaaca aggcctgcct gcgcacggtt
    51421 ttgcccgcaa cctgccgtgg acgctgaaat cacatcgtga agatgctgat ggcgtagcgc
    51481 tgacttttga attgacacaa agcgaagaga cgaaaaaatt ctggccgcac gactttacgc
    51541 tgttagcgca tttccgcgtg ggtaaaacct gtgaaatcga tcttgaagca cacggcgaat
    51601 ttgaaaccac ctctgccctg catacctact ttaacgtggg tgatatcgct aaggtaagcg
    51661 tcagtgggct gggcgatcgc ttcattgata aagtaaatga cgcgaaagaa gatgtactga
    51721 ccgacggtat tcagaccttc cctgaccgta ccgatcgcgt atatctgaat ccgcaagatt
    51781 gcagcgtgat taatgatgaa gcgctgaatc gtattatcgc cgtaggccac cagcatcatc
    51841 tgaacgttgt aggctggaac ccgggaccgg cgctttcagt tagcatgggc gatatgccgg
    51901 acgatggcta caagaccttt gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag
    51961 tgaccaaaga aaaacctgca catctggcgc aatccattcg cgttgcgaaa cgttaattta
    52021 cgttaatgtt gtgtgccggg tgcgatgcat ccggcacgta tcaacatcac accatatcca
    52081 gcgcagtttt tccttttggt gccggatatg ctttgtccag catggctaat tccgctgacg
    52141 aaagttcgac ctcaagcaca gccgcattct gttggacatg ggcaatcgtg gccgcttttg
    52201 gaatcgccat cacaccctga tggctgatta cccacgccaa caatacttgt gccgcgctga
    52261 tattgtgagc atgtgcaatt tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc
    52321 gcccggcctg ggctaacgga ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg
    52381 ggagtagatc gtactcaatc cctcgtgaac cgagatggta aagcacctga ttagtggcac
    52441 actgatttcc ccccgtcagc tgccagagtt cctgcatatc agcataatca aggttcgaaa
    52501 cgccccagcg gcggattttt ccttgggcga tcaatttttc catcgctgcg acagtctctt
    52561 caaaagcgaa actgccagac cagtgtaata agtagagatc gagataatca gtattgagac
    52621 ggcgtaaact ggcttcgcat gcatttatcg ctttttgccc gccagcattc cacggataga
    52681 ctttagagac gagaaagacg ttatcacgca gaccggttaa tgcttcccca accacctttt
    52741 cggcaccacc atcggcatac atttcggcgg tatcaatgag ggttaaaccg agctcaatgc
    52801 ccgcgcgtag tgcagcaact tctgttttac acttactggc atcttcgccc atataccatg
    52861 ttccctgccc tatggctggc agtgagacat cgccactaaa ttgaatcatt ttttgttgca
    52921 ttgtttcctc ccaggtattg caccaccgta atgcaaaaca gggcgtgacg ccctgttttt
    52981 atgcacaaaa tgccctggaa agatgcatta tcagaatttg taggtgatcc cggtagaaat
    53041 caggccagtc caggatttat ccaccatcgg gctgtcagta acttcatcag acagacgggt
    53101 gtagcgcgcg gtaccgtaaa cactccagtc gccgaggaag ttgtagctgg cgctcagctc
    53161 caggtaaggg ctccagctgt cgttcgggtt atagccacgc agaccgctgc gagcggactc
    53221 tttgcgcgat acgccataat agtattcgtt ctggttttcg ctgttccact gcacaccaat
    53281 acccggagtc acggtcaggc caccgttggt gtaacgatac aaccaggcca tatcccagac
    53341 gatgccgttg ctgttatcca gggtatcgcc agccagggtg gtacgcaggt aaccgtactg
    53401 ggtaaagtga gcataagaca gaccagccat catggtgctc ttacggtcat ccaggtgacg
    53461 catttggtga tcgccactgt ctttagcttt gaagtaaagc ggcgaccagt aagcggtaat
    53521 tgaaagttta tccgttgcgt cattccacag gtagtaacca ccacctaagc cacggaacca
    53581 gaagttatcg ccttcatagt tgattaccgg tactgggtaa acatcggtat cgtaatcttt
    53641 atatgggtgt tcaacgacac ctacgcctgc gcccagggaa aatttacctt cagcgtgcgc
    53701 tacgcctgca gacgttgcga taagcactcc aagtgccaga agtttgagtt tggtcacaat
    53761 taatcattcc ttaaacaaat gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa
    53821 cccaaccttt ttacgttttc attttttaaa gtaactgttt aattttcctg acgcggatga
    53881 caccgcgctt acagccaaat gaatttaacg ttactggcaa ccctggtctt tacattaatt
    53941 atgcaaaatt tatggatgag ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca
    54001 agttttccat ttgccatcta cgcttaattt tgaaggtgta tcaccgggca cgttgttctc
    54061 atcgtcgata aaatggcatg agagttgctg tgttttagca agagacgtcg ttcagtttac
    54121 ctcttccggg agcctctact attcatatga acggctctta acctgtgcta aaaaacgaaa
    54181 ggacggcata ccatgaatat attcgatcac tatcgccagc gatatgaagc tgccaaggac
    54241 gaagagttca cactgcagga gtttcttacc acttgtcggc aagatcgcag tgcttatgcc
    54301 aacgcggctg agcggctatt gatggctatc ggtgagcctg tcatggtcga tacagcccag
    54361 gaacccagac tttctcgact cttttctaac cgggtcattg cacgttatcc ggcatttgaa
    54421 gagttttacg gcatggaaga cgcgattgaa cagattgtct cttatctgaa acacgcggct
    54481 caggggctgg aagagaagaa acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa
    54541 tcatcgcttg ctgagcgact gaaatcatta atgcagctcg taccgattta tgtattgagc
    54601 gccaacggtg agcgtagccc ggtcaacgat catccgttct gtcttttcaa tccgcaggaa
    54661 gatgcgcaga ttctggaaaa agagtatggc attcctcgcc gctatctcgg caccatcatg
    54721 tcgccgtggg cggcaaaacg tctgcatgaa tttggtggcg atattactaa gttccgggta
    54781 gtgaaggtct ggccgtccat tctgcaacaa attgctatcg ccaaaacaga gcccggtgat
    54841 gagaacaacc aagacatctc cgcgctggta gggaaagtcg atattcgtaa actcgaacac
    54901 tacgcgcaga atgacccaga cgcctatggc tattccggcg cactgtgccg cgccaaccag
    54961 gggatcatgg aattcgttga gatgtttaaa gcaccgatta aagtgctgca tccgctgtta
    55021 accgccactc aggaaggtaa ctacaacggg acggaaggta tctccgccct gccgttcaac
    55081 gggattattc ttgcccactc gaacgagtcc gaatgggtca ctttccgtaa taacaaaaac
    55141 aacgaagcct tcctcgatcg tgtttacatc gtgaaggtgc cgtattgctt gcgcatttcc
    55201 gaagagataa aaatctacga gaaattgctt aatcacagtg aattgactca cgccccatgc
    55261 gcccctggca cgctcgaaac actgtcacgt ttttccattc tttcgcgcct gaaagagcca
    55321 gaaaactcca gcatttattc aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc
    55381 gatcccaaag ccaagtcgta tcaggaatat cgtgactacg ccggtgtcga tgaagggatg
    55441 aacggtctgt cgacgcgttt tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat
    55501 gtagaagtgg cggcaaaccc ggtccatctg ttctacgtcc tggaacagca gatcgagcgc
    55561 gagcagttcc cacaagagca ggcagaacgc tatctggagt tcctgaaagg ttatctgatc
    55621 ccgaaatatg ccgagtttat tggcaaagag atccagacgg cctaccttga atcctattcc
    55681 gaatatgggc aaaacatttt cgaccgttat gttacctacg cggatttctg gattcaggat
    55741 caggagtatc gcgatccgga taccgggcag ctgtttgacc gcgagtctct taacgccgag
    55801 ctggagaaaa tcgagaaacc ggcggggatc agtaatccaa aagatttccg caacgagatt
    55861 gttaacttcg tactgcgcgc cagagccaat aacagcggac gcaatccgaa ctggaccagc
    55921 tatgaaaaac tgcgcacggt tatcgagaag aaaatgttct ccaataccga ggagctgttg
    55981 ccggttatct cgtttaacgc caaaacgtca accgacgagc agaagaaaca cgacgacttt
    56041 gtcgaccgta tgatggaaaa aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg
    56101 tatttgcgcg tacgtaaatc gtcttaacaa ccctggcccg gtacacatgc taccgggcct
    56161 acaacgacag cgaaccgtgg gccagagaaa tggcaacaca ggcgtagcat acagttggca
    56221 aatgtagtac ggggggcata tgacctggtt tattgaccgg cgtctgaacg gcaaaaacaa
    56281 aagcatggtg aatcgccagc gttttttacg ccgttataaa gcgcaaatta aacagtcgat
    56341 ctccgaggcc attaataagc gttcggtgac tgacgtcgac agcggcgaat ccgtatccat
    56401 tcccacggaa gatattagcg aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg
    56461 cgtacatccg ggcaatgacc atttcgtcca gaacgaccga attgaacgtc cccagggtgg
    56521 cggcggaggt tccggcagtg gtcagggcca ggccagccag gatggtgaag gtcaggatga
    56581 attcgtcttt cagatttcga aagatgagta tcttgatctg ctctttgaag atttggcctt
    56641 accgaatctg aaacaaaacc aacaacgcca gctgaccgaa tataaaacgc atcgggcggg
    56701 ttataccgct aacggcgttc cggccaatat cagcgttgtg cgttccttgc agaactcact
    56761 ggcgcgacgc acagccatga cggcaggcaa gcggcgggaa cttcatgcac tggaagagaa
    56821 tttggccatc atcagcaaca gtgaacctgc gcagctgctg gaagaggaac gtctgcgcaa
    56881 agaaattgca gaattacgtg ccaaaattga acgcgtccct tttattgaca ccttcgattt
    56941 acgttacaag aactacgaga agcggcccga tccctccagc caggcagtga tgttttgcct
    57001 gatggacgtt tccggttcaa tggatcaatc cactaaagat atggctaagc gtttttatat
    57061 tctgctgtat ctgttcctca gcagaacgta taagaacgtg gaagtcgtat acatccgcca
    57121 tcatacccag gcgaaagaag tcgatgaaca tgagtttttc tactcgcagg aaactggcgg
    57181 caccattgtt tccagcgccc tgaaactgat ggatgaggta gtgaaagagc gttataaccc
    57241 ggcacagtgg aatatttacg ctgcacaagc atcggacggc gataactggg ccgatgactc
    57301 tccgctttgc catgaaattc tggcgaaaaa attattaccc gttgttcgtt attacagcta
    57361 tatcgaaatt acccgtcgcg cgcatcagac actgtggcga gaatatgagc atctgcaatc
    57421 tactttcgac aactttgcga tgcagcacat ccgcgaccag gatgatattt atccggtgtt
    57481 ccgtgaactg tttcataaac aaaatgcaac agctaaagac taaaactatc agccgggtca
    57541 ttatcgcctg gctgattttt agcttactgt aaattatctc atttattaca tacattagct
    57601 tacaat
//
LOCUS       DAZFKF010000023        57177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFKF010000023 DAZFKF010000000
VERSION     DAZFKF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.023"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..218
                     /gene="yigG"
                     /locus_tag="ACHKA1_001703"
     CDS             <1..218
                     /gene="yigG"
                     /locus_tag="ACHKA1_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HFO5679376.1"
                     /translation="VINMNCQRLRDSGFIYIKTYVFGTLAVYIISIITMIAEDFACSG
                     NGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            232..612
                     /gene="yigF"
                     /locus_tag="ACHKA1_001704"
     CDS             232..612
                     /gene="yigF"
                     /locus_tag="ACHKA1_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFO5679377.1"
                     /translation="MSKEYMNDGSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(655..1605)
                     /gene="corA"
                     /locus_tag="ACHKA1_001705"
     CDS             complement(655..1605)
                     /gene="corA"
                     /locus_tag="ACHKA1_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO5679378.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1757..1813)
                     /gene="ysgD"
                     /locus_tag="ACHKA1_001706"
     CDS             complement(1757..1813)
                     /gene="ysgD"
                     /locus_tag="ACHKA1_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO5679379.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            1975..2739
                     /gene="yigE"
                     /locus_tag="ACHKA1_001707"
     CDS             1975..2739
                     /gene="yigE"
                     /locus_tag="ACHKA1_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO5679380.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIIRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYVKGGAIPWQRYPFVTMISAERKG"
     gene            2822..3724
                     /locus_tag="ACHKA1_001708"
     CDS             2822..3724
                     /locus_tag="ACHKA1_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFO5679381.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTGRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAISFEQVGPELPAKVVELPYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSEKKYEGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCDK"
     gene            complement(3769..5931)
                     /gene="uvrD"
                     /locus_tag="ACHKA1_001709"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(3769..5931)
                     /gene="uvrD"
                     /locus_tag="ACHKA1_001709"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO5679382.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(6015..6731)
                     /gene="yigB"
                     /locus_tag="ACHKA1_001710"
     CDS             complement(6015..6731)
                     /gene="yigB"
                     /locus_tag="ACHKA1_001710"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO5679383.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(6731..7627)
                     /gene="xerC"
                     /locus_tag="ACHKA1_001711"
     CDS             complement(6731..7627)
                     /gene="xerC"
                     /locus_tag="ACHKA1_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO5679384.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(7624..8331)
                     /gene="yigA"
                     /locus_tag="ACHKA1_001712"
     CDS             complement(7624..8331)
                     /gene="yigA"
                     /locus_tag="ACHKA1_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFO5679385.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(8328..9152)
                     /gene="dapF"
                     /locus_tag="ACHKA1_001713"
     CDS             complement(8328..9152)
                     /gene="dapF"
                     /locus_tag="ACHKA1_001713"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO5679386.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(9189..9392)
                     /gene="yifL"
                     /locus_tag="ACHKA1_001714"
     CDS             complement(9189..9392)
                     /gene="yifL"
                     /locus_tag="ACHKA1_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5679387.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            9551..9693
                     /locus_tag="ACHKA1_001715"
                     /pseudo
     CDS             9551..9693
                     /locus_tag="ACHKA1_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            9855..10175
                     /gene="cyaY"
                     /locus_tag="ACHKA1_001716"
     CDS             9855..10175
                     /gene="cyaY"
                     /locus_tag="ACHKA1_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO5679388.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VRFR"
     gene            complement(10215..12761)
                     /gene="cyaA"
                     /locus_tag="ACHKA1_001717"
     CDS             complement(10215..12761)
                     /gene="cyaA"
                     /locus_tag="ACHKA1_001717"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO5679389.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPDRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            13148..14089
                     /gene="hemC"
                     /locus_tag="ACHKA1_001718"
     CDS             13148..14089
                     /gene="hemC"
                     /locus_tag="ACHKA1_001718"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO5679390.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            14086..14826
                     /gene="hemD"
                     /locus_tag="ACHKA1_001719"
     CDS             14086..14826
                     /gene="hemD"
                     /locus_tag="ACHKA1_001719"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO5679391.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            14848..16041
                     /gene="hemX"
                     /locus_tag="ACHKA1_001720"
     CDS             14848..16041
                     /gene="hemX"
                     /locus_tag="ACHKA1_001720"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO5679392.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMNNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPQAAPQADTPAA
                     APQGE"
     gene            16044..17240
                     /gene="hemY"
                     /locus_tag="ACHKA1_001721"
     CDS             16044..17240
                     /gene="hemY"
                     /locus_tag="ACHKA1_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO5679393.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            17919..19574
                     /gene="aslA"
                     /locus_tag="ACHKA1_001722"
     CDS             17919..19574
                     /gene="aslA"
                     /locus_tag="ACHKA1_001722"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO5679394.1"
                     /translation="MEFSFSPKRLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     DYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(19742..20977)
                     /gene="aslB"
                     /locus_tag="ACHKA1_001723"
     CDS             complement(19742..20977)
                     /gene="aslB"
                     /locus_tag="ACHKA1_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO5679395.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRYYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMVDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(21124..21200)
                     /locus_tag="ACHKA1_001724"
     tRNA            complement(21124..21200)
                     /locus_tag="ACHKA1_001724"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21164..21166),aa:Pro,seq:tgg)
     gene            complement(21243..21329)
                     /locus_tag="ACHKA1_001725"
     tRNA            complement(21243..21329)
                     /locus_tag="ACHKA1_001725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21293..21295),aa:Leu,seq:cag)
     gene            complement(21350..21425)
                     /locus_tag="ACHKA1_001726"
     tRNA            complement(21350..21425)
                     /locus_tag="ACHKA1_001726"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21390..21392),aa:His,seq:gtg)
     gene            complement(21484..21560)
                     /locus_tag="ACHKA1_001727"
     tRNA            complement(21484..21560)
                     /locus_tag="ACHKA1_001727"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21524..21526),aa:Arg,seq:ccg)
     gene            complement(21663..23048)
                     /gene="yifK"
                     /locus_tag="ACHKA1_001728"
     CDS             complement(21663..23048)
                     /gene="yifK"
                     /locus_tag="ACHKA1_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5679396.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(23239..23979)
                     /gene="wecG"
                     /locus_tag="ACHKA1_001729"
     CDS             complement(23239..23979)
                     /gene="wecG"
                     /locus_tag="ACHKA1_001729"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO5679397.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(23982..25334)
                     /gene="wzyE"
                     /locus_tag="ACHKA1_001730"
     CDS             complement(23982..25334)
                     /gene="wzyE"
                     /locus_tag="ACHKA1_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO5679398.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILIGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(25331..26410)
                     /gene="rffT"
                     /locus_tag="ACHKA1_001731"
                     /gene_synonym="wecF"
     CDS             complement(25331..26410)
                     /gene="rffT"
                     /locus_tag="ACHKA1_001731"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO5679399.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDNCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPKLWLALLSGGIKSR
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDSSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(26407..27657)
                     /gene="wzxE"
                     /locus_tag="ACHKA1_001732"
     CDS             complement(26407..27657)
                     /gene="wzxE"
                     /locus_tag="ACHKA1_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO5679400.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVIGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(27659..28789)
                     /gene="rffA"
                     /locus_tag="ACHKA1_001733"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(27659..28789)
                     /gene="rffA"
                     /locus_tag="ACHKA1_001733"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO5679401.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(28794..29468)
                     /gene="rffC"
                     /locus_tag="ACHKA1_001734"
                     /gene_synonym="wecD"
     CDS             complement(28794..29468)
                     /gene="rffC"
                     /locus_tag="ACHKA1_001734"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO5679402.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(29446..30327)
                     /gene="rfbA"
                     /locus_tag="ACHKA1_001735"
     CDS             complement(29446..30327)
                     /gene="rfbA"
                     /locus_tag="ACHKA1_001735"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO5679403.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(30346..31413)
                     /gene="rffG"
                     /locus_tag="ACHKA1_001736"
     CDS             complement(30346..31413)
                     /gene="rffG"
                     /locus_tag="ACHKA1_001736"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO5679404.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKSLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPQGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(31413..32675)
                     /gene="wecC"
                     /locus_tag="ACHKA1_001737"
     CDS             complement(31413..32675)
                     /gene="wecC"
                     /locus_tag="ACHKA1_001737"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO5679405.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(32672..33802)
                     /gene="wecB"
                     /locus_tag="ACHKA1_001738"
     CDS             complement(32672..33802)
                     /gene="wecB"
                     /locus_tag="ACHKA1_001738"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO5679406.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(33858..34904)
                     /gene="wzzE"
                     /locus_tag="ACHKA1_001739"
     CDS             complement(33858..34904)
                     /gene="wzzE"
                     /locus_tag="ACHKA1_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO5679407.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(34916..36019)
                     /gene="wecA"
                     /locus_tag="ACHKA1_001740"
     CDS             complement(34916..36019)
                     /gene="wecA"
                     /locus_tag="ACHKA1_001740"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO5679408.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(36259..37518)
                     /gene="rho"
                     /locus_tag="ACHKA1_001741"
     CDS             complement(36259..37518)
                     /gene="rho"
                     /locus_tag="ACHKA1_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO5679409.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(37603..37704)
                     /gene="rhoL"
                     /locus_tag="ACHKA1_001742"
     CDS             complement(37603..37704)
                     /gene="rhoL"
                     /locus_tag="ACHKA1_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO5679410.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(37845..38174)
                     /gene="trxA"
                     /locus_tag="ACHKA1_001743"
     CDS             complement(37845..38174)
                     /gene="trxA"
                     /locus_tag="ACHKA1_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO5679411.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            38305..39570
                     /gene="rhlB"
                     /locus_tag="ACHKA1_001744"
     CDS             38305..39570
                     /gene="rhlB"
                     /locus_tag="ACHKA1_001744"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO5679412.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            39704..41188
                     /gene="gppA"
                     /locus_tag="ACHKA1_001745"
     CDS             39704..41188
                     /gene="gppA"
                     /locus_tag="ACHKA1_001745"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO5679413.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(41235..43256)
                     /gene="rep"
                     /locus_tag="ACHKA1_001746"
     CDS             complement(41235..43256)
                     /gene="rep"
                     /locus_tag="ACHKA1_001746"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO5679414.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            43473..43922
                     /locus_tag="ACHKA1_001747"
     CDS             43473..43922
                     /locus_tag="ACHKA1_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO5679415.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFITTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNIINDLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            44117..44398
                     /gene="ppiC"
                     /locus_tag="ACHKA1_001748"
     CDS             44117..44398
                     /gene="ppiC"
                     /locus_tag="ACHKA1_001748"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO5679416.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(44444..45919)
                     /gene="ilvC"
                     /locus_tag="ACHKA1_001749"
     CDS             complement(44444..45919)
                     /gene="ilvC"
                     /locus_tag="ACHKA1_001749"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO5679417.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            46069..46962
                     /gene="ilvY"
                     /locus_tag="ACHKA1_001750"
     CDS             46069..46962
                     /gene="ilvY"
                     /locus_tag="ACHKA1_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO5679418.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(47014..48558)
                     /gene="ilvA"
                     /locus_tag="ACHKA1_001751"
     CDS             complement(47014..48558)
                     /gene="ilvA"
                     /locus_tag="ACHKA1_001751"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO5679419.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(48561..50411)
                     /gene="ilvD"
                     /locus_tag="ACHKA1_001752"
     CDS             complement(48561..50411)
                     /gene="ilvD"
                     /locus_tag="ACHKA1_001752"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO5679420.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(50476..51405)
                     /gene="ilvE"
                     /locus_tag="ACHKA1_001753"
     CDS             complement(50476..51405)
                     /gene="ilvE"
                     /locus_tag="ACHKA1_001753"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO5679421.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(51425..51688)
                     /gene="ilvM"
                     /locus_tag="ACHKA1_001754"
     CDS             complement(51425..51688)
                     /gene="ilvM"
                     /locus_tag="ACHKA1_001754"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO5679422.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(51685..53331)
                     /gene="ilvG"
                     /locus_tag="ACHKA1_001755"
     CDS             complement(51685..53331)
                     /gene="ilvG"
                     /locus_tag="ACHKA1_001755"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO5679423.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLSTTKMPATCTLKGLGAVAADYSYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLSDEHAWRYDHPGDTIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(53334..53384)
                     /gene="ilvX"
                     /locus_tag="ACHKA1_001756"
     CDS             complement(53334..53384)
                     /gene="ilvX"
                     /locus_tag="ACHKA1_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO5679424.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(53471..53569)
                     /gene="ilvL"
                     /locus_tag="ACHKA1_001757"
     CDS             complement(53471..53569)
                     /gene="ilvL"
                     /locus_tag="ACHKA1_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO5679425.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            53922..55441
                     /gene="yifB"
                     /locus_tag="ACHKA1_001758"
                     /pseudo
     CDS             53922..55441
                     /gene="yifB"
                     /locus_tag="ACHKA1_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(55466..55804)
                     /gene="maoP"
                     /locus_tag="ACHKA1_001759"
     CDS             complement(55466..55804)
                     /gene="maoP"
                     /locus_tag="ACHKA1_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO5679426.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            55923..56762
                     /gene="hdfR"
                     /locus_tag="ACHKA1_001760"
     CDS             55923..56762
                     /gene="hdfR"
                     /locus_tag="ACHKA1_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO5679427.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(56858..56933)
                     /locus_tag="ACHKA1_001761"
     tRNA            complement(56858..56933)
                     /locus_tag="ACHKA1_001761"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56898..56900),aa:Trp,seq:cca)
     gene            complement(56942..57018)
                     /locus_tag="ACHKA1_001762"
     tRNA            complement(56942..57018)
                     /locus_tag="ACHKA1_001762"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56982..56984),aa:Asp,seq:gtc)
     gene            complement(57073..>57177)
                     /gene="rrf"
                     /locus_tag="ACHKA1_001763"
     rRNA            complement(57073..>57177)
                     /gene="rrf"
                     /locus_tag="ACHKA1_001763"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        14036 a        15427 c        14503 g        13211 t
ORIGIN      
        1 tcgtcatcaa catgaattgc cagcgactcc gtgatagtgg tttcatctat ataaaaactt
       61 atgtttttgg tacattagca gtatatatca tctctatcat cacaatgata gccgaagatt
      121 ttgcctgttc aggcaatggt tcgatgattt ttttgatttg ttatttttcc actttcagta
      181 tgctaatgct tgcacctact gattcatcaa aacaatagtt atggagtatt tatgagtaag
      241 gaatatatga acgatggttc attaagtgaa aaatggaaat accgatttaa tttttatgat
      301 caacatggct ttcctggatt ctggggagca acaccagaat ataaagcagc atttaaagca
      361 ttaaaagtaa gacaacgatt aactattcaa atgaatttca ttgctttctt ctgttcctgg
      421 atctatttat ttgttctggg cttatggaaa aaagcaatta tagttctatt acttggcatt
      481 ctatctctat ttgttggtgc attaattggc gttaacattc tgggaattgc cgtagcagcc
      541 tatgttgctg ttaacacgaa caaatggttt tatgagaaag aggtaaaagg gttaaatacc
      601 tggagccttt gaataacagg tggcttagcc aggctaaacc accgctctcg ttttttacaa
      661 ccagttcttc cgcttaaagt acagatacgg tgccaggccc gcgaggatca taaagataat
      721 cgcgcctggg tagccgaaac tccacttcag ttctggcata aactcaaagt tcatcccgta
      781 gctggaagca accagcgttg gcggcaggaa cacgacggat accaccgaga aaattttgat
      841 gatgcggttc tgctcgatgt tgataaaacc cattgccgcc tgcatcagga agttcacctt
      901 ctggaacagg gattcgttat gcggcagcag ggattcgata tctcgcagga tttcacgcgc
      961 ctgctccagt tgaccacccg gtaaacgcgc cttacgcacc aggaagttga gcgcacgctg
     1021 ggtatccatc agacacaggc gcactttcca gccgatatct tccagttccg ccagagtgga
     1081 gagcgcctcg tcgtactcat cgccctgatg cccttccata atcacccggc tcaactgctc
     1141 caggtcgcta tagatatttt caatttcatc tgccaactgt tcgattttgg tttcgaacag
     1201 atccagcagc agctcgtagg cgttaccgtc caccattgac tggctacggg cgcgcatacg
     1261 atacagacgg aatgcaggca gttcacgctc acgcagagta aacagacgac catcacggat
     1321 ggtaaatgcc acagtggagt taccggcgtg atcttccgca tcttcaaaga agaagaagga
     1381 gtgaatatgc aggccgtcat cgtcttcaaa gaaacgtgcc gatgcttcga tgtcttccag
     1441 ttccgggcgg gttgccaggc tctggccaag ttcagattgt acgcgcagtc gctcgtcgtc
     1501 gtccggttcg acaagatcaa tccatactgc atttacaagg ggttgtgact cttcgacttc
     1561 cagccgggtc agtcggttat tttccagttg aaatgcgctc agcatgaccg ggactcccaa
     1621 tgcttaaaat atcggacagt tcggtgggca cacagaaaca aattgggttt cagaccatta
     1681 aacagcctga ctcagcgcga cgggaaaaat tgaggtcgct gacaaccgct aaggctatca
     1741 gcaaaaaggg atagccttag gagttgatcc tggatgacag gatagtgagc cagtatctac
     1801 tgggtgtgtc caaggcgaat gtcctcttag agtgatcgtg cgcgcatgtt acgccagcaa
     1861 aattttgccg tcaacacgca acagaacacc acagaacaat agttgaactt cgcgcttttt
     1921 ggtactcgtc aaggtcgcta acaggataaa aaaatcccac atccaggacc tataatggcg
     1981 catcggctac tcattggtaa aggaatgatc accttgaatc tcaaacggat ttttctcgcc
     2041 ctcaccttac ttcccttgtt tgctgttgcc gctgatgatt gcgcactctc agatccgacg
     2101 ctgaccgtac aggcgtatac cgttaatcct caaacagagc gggtgaaaat gtactggcaa
     2161 aaagccaatg gcgaagcgtg gggaacttta catgctctgc tggcggatat gaatagtcag
     2221 ggtcaggtgc agatggcgat gaacggcggc atctatgatg aaagctatgc gccgctcggt
     2281 ttgtacatcg aaaacggtca gcagaaggtg gcgttaaatc tcgcttcagg tgaagggaat
     2341 ttctttatcc gtcctggcgg cgtgttttat gtcgcgggag ataaagtcgg catcattcgt
     2401 ctggatgcct tcaaaaccag taaagagatt cagtttgcgg tgcagtcagg gccaatgttg
     2461 atggaaaaca gtgtaattaa tccgcgtatt catcccaacg tcgcctcacg taaaattcgt
     2521 aacggtgttg ggattaataa acatgggaat gccgtgtttt tgttgagcca gcaggcgaca
     2581 aatttttatg attttgcctg ttatgccaaa gcgaaactga acgttgagca gctgctttat
     2641 cttgatggca cgatttcaca tatgtatgtg aaaggcgggg caatcccgtg gcaacgctat
     2701 ccttttgtca ccatgatttc cgccgagcgg aaaggttaag cattgtacac tcgaggaggc
     2761 cagacaaact ggggctggct acacagctgg aaaatcatca gataaatgaa gggaaacaaa
     2821 aatgaaaaaa acgctcgcaa cgttattttt attaacgtgt ctgggatcat catccgctta
     2881 tgcggataac gctttgattt tacaaactga ttttagcctg aaagatggtg ccgtctcggc
     2941 gatgaaaggg gtcgcattcg gtgttgacca taatcttaaa atctttgatt taacacacga
     3001 aattccaccg tataacatct gggaaggcgc ttaccgcctg tatcagaccg ccagttattg
     3061 gccacaaggt tcggtgtttg ttagcgtggt cgatccaggt gtaggtacag gccgtaaatc
     3121 ggtagtgctg aaaaccaaaa acggtcaata cttcgtttca ccggataacg gtacgctcac
     3181 tttggtggcg gagtcgctgg gtattgagag cgtccggcaa atcgatgaaa aaaccaaccg
     3241 cctgaaaggt tccgaaaagt catatacctt ccatggtcgc gatgtatatg cctacacagg
     3301 ggcgcgtctg gcttccggcg cgatttcctt tgagcaggtg gggccagagc ttccggcaaa
     3361 agttgtcgaa ctgccttatc agaaagcgaa agcgacaaaa ggtgaagtga agggcaatat
     3421 cccgattctc gatattcagt acggtaacgt ctggagcaat atcagcgacg agttgctgaa
     3481 tcaggcagga atcaaactca acgatacgtt atgtgtgacc atttccgaag gttccgagaa
     3541 aaaatacgaa gggaaaatgc cgtatgtcgc cagcttcggc gatgtgccgg aaggacagcc
     3601 gatggtgtat ctgaacagct tattgaatgt ttccgtggcg ctgaatatgg ataatttcgc
     3661 gcagaaacat caggttgcgt ctggtgctga ctggaatatt gatgtgaaga aatgtgataa
     3721 gtaaggtgct gatgccggat gcggtgtggc gcaccgcatc cggcaacgtt ataccgtctc
     3781 cagccgggcg taagccgcca ccagccattt aataccctgg ccctggaatg ccacctgcaa
     3841 acggctatgc tcgccgctgc cttccatatt gacgatagtg ccttcaccaa acttggcgtg
     3901 gcgcacgcgc tggccaagtt tgtagccgct gtcgttctcg accatcggcg tacccatacg
     3961 ctgatggctg accgggcggc ttaccgtggc gcgcaggcgc acctcttcca cacactcttc
     4021 cggcaactca ccgataaagc gcgacgggcg atggtaaacc tctttgccat acagacggcg
     4081 agtttccgcg taggtcagcg tcagtttctg catcgcacgg gttacgccaa cgtaggccag
     4141 acggcgttct tcttccagac gaccgccttc atccagcgac atctggcttg ggaacatgcc
     4201 ctcttccata ccgacgataa acacctgcgg gaactccagg cctttcgccg agtgtagcgt
     4261 catcaactgc accgcgtcct gccaggtatc cgcctgcccc tcgcccgctt ccagcgccgc
     4321 atgggagagg aacgcctgca gcggcattaa atcttcgtct tcttcgttgt agctgaactg
     4381 gcgcgttgcc gtcaccagtt cctctaagtt ttcgatacgc gtctgacctt tttcgccctt
     4441 ctcctgctcg tacatggtac gcaggccgga gtctttaatt acccggtcag tctgtacatg
     4501 cagcggcata tcggcagttt cctgcgctaa ggcgtcgatc agttccataa accgctgcaa
     4561 ggcactggct gcacgtccgg cgagggcttt ttcctgcaac agttcacgac atgcctgcca
     4621 gagtgttaac tggcgatcgc gcgatgtctg acgtaccacg tccagcgtcc ggtcaccaat
     4681 accccgcgtt ggtgtattca ctacgcgctc aaaggccgcg tcgtcgttgc ggttggcaat
     4741 caggcgcaga tacgagagcg catctttgat ttcctggcgt tcgaagaagc gcatcccgcc
     4801 gtaaatacgg tacggcatac tcgcctgtaa taaggcctct tccagtacac gcgactgggc
     4861 gttgctgcgg tagagaatgg cgcactcggc aagcgctccg ccgttgtcct gccaggtttt
     4921 gatgcggtta accacaaaac gcgcttcatc gagttcgtta aaagcgcaat agagggaaat
     4981 aggctcaccg tccgcgccat cggtccacag ttttttaccc agacgcccgt tattgttttc
     5041 aatcagggcg ttagcggcgc tcagaatatt gctggtagag cggtagtttt gctccagacg
     5101 aatagtttcg gcaccgggga aatcattaag gaaacgctga atattctcca cctgcgcccc
     5161 gcgccagccg tagattgact ggtcgtcatc accgacgatc atcactttgc cggtgtcgcc
     5221 cgccagcagg cggatccacg cgtactgaat gttgttggta tcctggaatt cgtccaccag
     5281 gatgttagta aaacgttcgc ggtagtgttg caggatatgc ggcttgttaa gccacaactc
     5341 gtgagcacgc agcagcagct cggcgaagtc caccaggccc gcacggtcgc acgcttcctg
     5401 atacgcctga tacactttct gccaagtctg ctccaccgga ttaccgtagc tttgaatatg
     5461 atgcggacgt aagccttcat ctttctggct gttgatgtac cacattgcct ggcgcggcgg
     5521 ccactgcttc tcgtcgaggt tcatggcttt gatcagacgc ttaagcaggc gtagctggtc
     5581 ttcactgtcg aggatctgga aatcctgcgg cagattggcg tccatatggt gcgcacgcag
     5641 caggcggtgc gccagcccgt ggaaggtgcc gacccacatg ccgccctggc tggtgcccat
     5701 cagttgcccg atacgatgac gcatctccgc cgccgctttg ttggtaaacg tcacagccat
     5761 aatcgagtat ggcgagcagt tttccacgct catcagccag gcgatacgat gcaccagtac
     5821 gcgcgtctta ccactgcccg cgcccgccag caccagaagg ttgctgcgtg gcgcggccac
     5881 cgcttcgcgc tgtttgtcat taaggctgtc gagcaggtaa gaaacgtcca ttggcaccgc
     5941 cgcgtaaaaa taggttgggc ggagaagcgc aacgccctcc gccaaaaagc tgggtatata
     6001 tacagatttg ctgattatat cagcgaggtc agagatgcca accgggaaat ttccagatgc
     6061 ggcagtaaac ggctatccca ggtttgcatc agatcgccat tttccggtct gatccaacaa
     6121 gcctgcattc cgctgcgaat tgccccaccc acgtcagtgg tgagatcgtc cccaacatgt
     6181 aagatctcgc cgatcggcac gttgagtttt tccgcagcca aaaagtacat atcgctgaac
     6241 ggttttgagc gcccgtgcgg gccagcgcgc agcacaaact caaaataatc ccccaaacca
     6301 aacagttccg gctgggcgtt accgttggtg atcgccacca gcggccattt tttcgccagc
     6361 tgttttaagg tgtcgtgagt ttgctgcggg acgtcgattc ggctgcgcca tttggcaaag
     6421 ttaatcattg ctgcgtgtgc gcctgcactg gcttcttcgg cactcagccc ggcgtcgagc
     6481 atcgcttgtt caatcgaacg aaaacgccaa cgcgtcacgt cgtgataaat ctcgggttcc
     6541 gcttcccgta ccgcctggcg caggcgttgc agatcttcat tctggaagct gcgcagcgcc
     6601 ggatgataat tttgcacaaa ggtaagcgcc tctcgctcgg tgcgcaaaat caccggacgg
     6661 ttatcgtaaa gggtatcatc caggtcaaag gtgagcgccg agatgcgccc caaaggccgg
     6721 taaaaacgca ttatttcccc cgtttggcgc gtggatgcgc cgcatcgtac accgaggcaa
     6781 ggtgttgaaa atcaagatga gtatagattt gcgtggtgga gaggttggca tgacccagca
     6841 gctcctgcac accacgaaga tcgccgctcg actccagcat atgcgtagcg aacgagtgac
     6901 gtaatttatg cgggtgaacg tgattattca gcccttgttt tatgccccat tcggcaaagc
     6961 gtttctgcac attacgcgcg gagatacgct tgcccagttt cgacagaaaa agcgcgtcgt
     7021 cttcgctacc aaacaggtcg cgcaaatcaa gccagtgctc aatccacgcc acagcgttgc
     7081 gaccaatcgg caggcggcgc tctttgctgc ctttccccat tacccacact tcaccagact
     7141 ccaggtcgag gtgtttgata tccagcccca ccaactcgga aagacgcaga ccagcgccgt
     7201 acatcacttc cagcattgca cggtcgcgta cagcgagggg atcgttgata tcaatatcca
     7261 gcagccgatt catatcgtcg acgtcgatgt ttttcggcag atgacgcggc gctttcggtg
     7321 ccgaaacacc tttcgccggg ttagctttga gttcgttctg gctgaccagc cagtcaaaaa
     7381 agctacgtag cgcagaaagc cgtaacgcga ggcttgctgc tcccagccct ttacggcgac
     7441 tgcgtacagc aaaattgcgc accatcgctg catcgcattg ttgccagctt tgcaggccgt
     7501 tttcgctggc aaaattgatg atcgcctcaa gctgacgctg gtagttaagt agggttatcg
     7561 ggctaagctg acgctccacg ctcagataac gtaggtagcg ttctacatcg gtgtgtaaat
     7621 cggtcatacg cgttcaatcc aacgctccag aagctccggc aacatcagcg caatttcatg
     7681 aagtaactgc gttccctgcc cttgttgata gtgactggca tcgcgactgg taaacagcac
     7741 gacacccaaa tcagcatcgc ttcccagcat cgacatcgcc accgatccca ccgctttcgc
     7801 ttccggtagc accaccagca gctctggtcc gttaagcggc ccaagatagt gctgttcctg
     7861 ccccaaacgc tgaatacgca gcggttcgaa agactgacgg cttaatgcca gatgagtgtg
     7921 gttcgacggc gcacctaagc gccagcgatc cggaaacagg cgcagactcg cacctgccag
     7981 gccgagatcg cgcgcccagc ggtgaaagcg catcagcata tcgtcgagac tgctggcggc
     8041 ggtgagactg cgctgcaggt agagtaagcg ataaaacagg ccttcgttgg cgatagcctg
     8101 ttccatcaac agcgccatgt tctcttccag aacatgaata tgattacgtg cgcgggccat
     8161 gtgccactcg accaacgaaa cggtgccgcg taccggatgc ggcacacgta tcgcttctac
     8221 tgcgcgcgca ttgcggataa aaaactcagg atttttaatc agataatcga caaccgcccg
     8281 gtcatcaagc tccgtgagtg tttcctgcag ttcttcccct ggttgcttca tagatgaata
     8341 aatccgtcgt agacatgtac cgccgggcca gtcatatata acgggtgacc cggacctttc
     8401 caggcgatat caagacgacc gccggggagt tccacgcgta cttcttcggc cagcaaacct
     8461 tgctgaatcc ctaccgccac cgccgcacat gcgccgctgc cgcaggcctg ggtttctcct
     8521 gccccacgct cataaacgcg taaacgaata tgctcgcgct taaccacttg cataaaaccg
     8581 atattggctc gctccggaaa acgctcgtgg ctttccagaa caggaccaag cgtttctacc
     8641 gccgcggtat cgacatcatc gacctgaatc acgcaatgcg gatttcccat cgacaccacg
     8701 ccgcataaga ttgtctgctc ggcggcgcgc ataatgtagg tcttttccgc tttgttagcg
     8761 cgaaacggca cagcggaagg ttcgaagttg ggttcgccca tatttacgcg gaccagatca
     8821 tcatcggtga cggtcagaac catccgcccg ttggcggtgc tgacgcggat atcgcgctta
     8881 ttggtcagcc ctttcaggcg cacaaaacgg gcaaagcagc gcgcaccgtt gccgcactgc
     8941 gccacttcac tgccatcagc attgaaaatg cgatagtgaa aatccagttc aggatcatac
     9001 ggcggctcaa ccaccagcag ctggtcaaac cctaccccca ggtgccgatc cgccaggcga
     9061 cgaatcagct ccggtgaaaa aaagacattc tgcgttaccg cgtcgacgac cataaaatcg
     9121 ttgccaaggc catgcatttt cgagaactgc atcatttact ccaatcacgc gggtacagaa
     9181 actgactttt agtaattcac ctgggatgga ccatcgccag tggcgcgatc gtttttatcc
     9241 ggcaccgtgg attgcgtttg cgtctctacc ggtttggtcg gcggcggtgc gtttttatct
     9301 gcaggcggga aatagagcgg acctttcaga ccgcagcccg tcaggctgaa gagagtaagt
     9361 aatacagtga gtgccttaaa cacgtttttc attatagatt gcctgtaaag ttcatcgctt
     9421 tctgctttct atcatcgcag gagaaggcgt aaaagcaagc gtttagcgac tctttatgaa
     9481 gtctgaaaag tgaggaaatg ctgcgagtat aacgcttact cgtggggatt tatgttttgc
     9541 tggtagtttc gtgatcggca cctcgcgcca ggatggcttt atgcactatc gcaatgtggt
     9601 ttgtttgttg gcttttttag ttccgtcagc gtgaggatgc tcgctcggta aacctgatca
     9661 taatgtttac cagaaacagg gaaaggcgtg gtgtaactgc gccctaatga agagacaatt
     9721 ctttcgctac acagagacat cattattccg gtgacggatg aatcatgccg taacgcagcc
     9781 gcagaggtgc aaactttgaa gccctgaccc catgccgcta tactgccgcc atcacttaaa
     9841 aacaggatac aacaatgaac gacagtgaat ttcatcgcct ggctgatcaa ctgtggctga
     9901 ccattgaaga gcgtctggac gactgggatg gcgacagcga tatcgactgc gaaatcaacg
     9961 gcggtgtact gaccattacc tttgaaaacg gcagcaaaat cattatcaac cgccaggaac
    10021 cgctgcacca ggtatggctg gcaaccaaac aaggcggcta ccattttgat ctgaaaggcg
    10081 atgagtggat ttgcgatcgc agcggcgaaa ccttctggga tttgctggaa caggcggcga
    10141 cgcagcaggc gggtgaaaca gtcagattcc gctaagattg catgccggat aagcctcgct
    10201 ttccggcacg ttcatcacga aaaatactgc tgtaatagcg gcgtatcgtg atcctgattg
    10261 gcaggcggca tgttaccgat agattttgtg cggaacggaa tcacctgttc acgaccatca
    10321 accttcacaa tctgatagaa ctgcggcagg ttgaagttga tgaagcttga gccgtaggta
    10381 aaacggtcat gcgatgacga gtagaagcga ctgacgtcac gtaccagctc ctctttgctg
    10441 ccttcgcagt ggtgatacac ctcaacccgg ttgctttcgt cgagaatgta gatattaaag
    10501 ccattctcat cttgcgtttc ttcgaaaaag aactggatga tcccttcgct ggcaaagccg
    10561 tccaccaccg ccggtaattt gacgtgattg gtttcaacct gcactgacag gccgtgcagt
    10621 ttgttatgcg aaatagcgcc ataaaactcg atggcgttct ccagtttctg taccgataca
    10681 ttcaggcgtt cgaagaacaa gccccaggtt tgaccagaaa cgcgcagcgc cttaaaacgc
    10741 ccggtttcct ggcgggtgct ggaaagacgc aattcaatac actcagaaac cagttgctgc
    10801 acgcgagtac gaattaagcc gcgcagatgc tggctataac agaagacttc cacgctatct
    10861 ggcggtgcgg cgtcctgatg cattttgccg agaatagttt tcagggcttc gatcatcgat
    10921 tgctcgccgt tgaagtgcag cgtacgcact tcgttccacg agttgcggta cagcaggtca
    10981 acgctaccta ccaggcaatt ttgattctcg ccaaagctga agacatccag cttacggaaa
    11041 tcgaaatgca ccacctgatt gcggaacgcc gctgtcgggt catattccag gttaacgata
    11101 atcgccagat ggcggatctc acacgggcta tagagcgcct tcggtgtcgg tgcaggtaag
    11161 cgcagcggga aatggtgcga cacgtcggcg accatctcct gcaacttagg caaatcgaca
    11221 atgccgttac ctttaatata caaacgggtg cgcgaggtca gcaggccatt aaaccatgcc
    11281 cacgccacca gcttattcag gtaacggtta tattccagcg gctgatggct gatgatcgac
    11341 tcaatatttg gcgcgcggtt atacagatac caacctgaac ggttagcccg gtccggcggc
    11401 acataaataa aggtcagatt cggttccgag agatcgggtg aaatctgcgg gtttaccagc
    11461 gtcactttac ctggtaatgc ttcaaacgcg gcatacagct tacgcgtcag cacgccgata
    11521 tcctgcggac tggcggagac gctaaggtta ttgcgacgcg caaagcggat cagattacgg
    11581 tagctctgca tcatcgcgtc gagcaactcg ttgtgcgcct cacgcacctg atcaatcttc
    11641 cagttagcgc ggttatcgag cattgccaga cgagcttcgt cccaacccca ctcgctcact
    11701 aactggctca acactgcgcg acgccagcct acgcaggcgc gttcacggct gagcttttcg
    11761 cacactttta aatagaagca gcgacgtact aaatccagac gggtaaaatc ttcaatcgcc
    11821 gtcaggtatt cagtaacacg ctccagcatc atgcagtatg gatcgagacc aaacgataca
    11881 atctcgccgt cgtgcaaacg ctgtttgata tctttcgcca gcagacgtgg gttcgggtat
    11941 tcccaggaat aggcttccag cagcagtgtt ttcagtaccg ctttgtatgg ggaatcgata
    12001 ctcttgtaga gctgccaaag gctggcacca aagtactctt cagcagaaag cgagcttaag
    12061 ccaccgagat ccagccattc atttggcgtc agcacgcctt gtgcgtagag cgtcattacg
    12121 tagtcatcgt aatgctcttc ttcgtcgcac ggcaccatat tccacagaat acgcttaccg
    12181 gcgagacgca cggcggtacg ataaaattca tcaagcagca gtatatgctg ggtggagcca
    12241 caatcttcgc cccccagact gccactctcg ttgtggcgaa aacggttttc atcaatcagg
    12301 aagaagctaa cttccacacc cagcgaggcg gcccagcttt ccagcaggct gcatttacgt
    12361 tgtagcaact ggcgctcttc gctatcgagc caggattgat gacagaccca gatatccagg
    12421 tcagaggaac aactttgccc tacggacgag gtgctgccca tggtgtatac accagtaatt
    12481 ggaagctcac ctttcggcgg gtcctgtact gacattccac gatacagttc aagctcgttc
    12541 aggtagtggc gttgagtttc atcaggcgtg taaaggcaaa tgcctttggg aacgttacca
    12601 tcaaggtaac ccggcattag cggatggtga tagtgcaaca atgtcggcag tagactgtag
    12661 acctgttgga atgcaggccc catagcagca agcgcgcgat ccacacgcaa ttgatttatg
    12721 gcatccagtc tctgtttcag agtctcaata tagaggtaca agacgtatcg cctgatttgc
    12781 tacccgtcat gactgtgatt ccgccaacat caacggtaac acgcggcatt cgggatattt
    12841 cgtatgtcaa aggtaaccgt taccactttt cgcgcctggt ttttttagtt tcacgacgaa
    12901 aaaatggtct aaaacgtgat caatttaaca ccttgctgat tgaccgtaaa gaaagatgcg
    12961 ctacatacaa gtgtagcacc gtttattctc tgtaaattcc ttattacgac ggcgtgaaac
    13021 gcctgtcagg atccactgcc agacctcatt ttacggtttg cgcaggcgtc tacgtttcac
    13081 cacaacactg acatcactct ggcaaggatg ttaggatgga ccacggatga taatgacggt
    13141 aacaagcatg ttagacaatg ttttaagaat tgccacacgc caaagcccac ttgcactctg
    13201 gcaggcacac tatgtcaaag acaagttgat ggcgagccat ccgggcctgg tcgttgaact
    13261 ggtaccgatg gtgacgcgcg gcgatgtgat tcttgatacg ccgctggcga aagtcggcgg
    13321 aaaaggctta tttgtaaaag agctggaagt cgcgctcctc gaaaatcgcg ccgatatcgc
    13381 cgtacattca atgaaagatg tgccggttga attcccgcaa ggtctggggc tggtcactat
    13441 ttgcgagcgt gaagatcctc gcgatgcctt tgtgtccaat aactatgaca gtctggatgc
    13501 gttaccggca ggcagtatcg tcgggacgtc cagtttacgt cgccagtgcc aactggctga
    13561 acgccgcccg gatctgatta tccgctccct gcgcggcaac gtcggcactc gcctgagcaa
    13621 actggataac ggcgaatacg atgccatcat tcttgccgta gccggactaa aacgtttagg
    13681 tctggagtcc cgcattcgcg ccgcgttgcc acccgagatt tctcttccgg cggtaggaca
    13741 aggtgcagtc ggtattgaat gccgccttga tgatacacgc actcgcgagc tgcttgccgc
    13801 gctgaatcac cacgaaactg cactgcgcgt taccgcagaa cgcgccatga atacccgtct
    13861 cgaaggcgga tgtcaggtgc caattggtag ctacgccgag cttattgatg gcgaaatctg
    13921 gctgcgtgcg ctggtcggcg cgccggacgg ttcgcagatt attcgcggtg aacgccgcgg
    13981 tgcgccgcaa aatgccgaac aaatggggat ttcgctggca gaagagctac tgaataacgg
    14041 cgcgcgcgag atcctcgctg aagtctataa cggagacgcc ccggcatgag tatccttgtc
    14101 acccgcccgt ctcccgctgg agaagagtta gtgagccgtc tgcgcacact ggggcaggtg
    14161 gcctggcatt ttccgctgat tgagttttct ccgggtcgac aattaccgca acttgctgat
    14221 caactggcag cgctggggga gagcgatctg ttgtttgccc tctcgcaaca cgcggttgct
    14281 tttgcccaat cacagctgca tcagcaagat cgtaaatggc cccgactacc tacttatttc
    14341 gccattggac gcaccaccgc actggcgcta cataccgtaa gcggacagaa gattctctac
    14401 ccacaggatc gggaaatcag cgaagtcttg ctacaattac ctgaattaca aaatattgcg
    14461 ggcaaacgtg cgctgatatt acgtggcaat ggcggtcgtg agctaattgg ggataccctg
    14521 acggcgcgcg gtgctgaggt cactttttgt gaatgttatc aacgatgcgc aatccattac
    14581 gatggtgcag aagaagcgat gcgctggcaa tcccgcgagg tgacgacggt cgttgttacc
    14641 agcggtgaaa tgttgcagca actctggtcg ctgatcccac aatggtatcg tgagcactgg
    14701 ttactacact gtcgactatt ggtcgtcagt gagcgtttgg cgaaactcgc ccgggaactg
    14761 ggctggcaag acattaaggt cgccgataac gctgacaacg atgcgctttt acgggcatta
    14821 caataactct cataacagga agccataatg acggaacaag aaaaaacctc cgccgtggtt
    14881 gaagagacca gggaggccgt ggacaccacg tcacaacctg tcgcaacaga aaaaaagagt
    14941 aagaacaata ccgcattgat tctcagtgcg gtggctatcg ctattgctct ggcggcaggc
    15001 gtcggtttgt atggctgggg taaacaacag gccgtcaatc agaccgccac cagcgatgcc
    15061 ctggctaacc aactgacggc attgcaaaaa gcccaggaga gccaaaaagc cgagctggaa
    15121 ggcattatta agcaacaagc tgcacaactt aagcaggcga atcgtcagca agaaacgctg
    15181 gcaaaacagt tggatgaagt ccaacaaaag gtcgccacca tttccggcag cgatgctaaa
    15241 acctggctgc tggctcaggc cgattttctg gtgaaactcg ccggacggaa gctgtggagc
    15301 gatcaggacg tcacgaccgc tgcagcgttg ctgaaaagtg cagacgccag cctggcggat
    15361 atgaatgacc cgagtctgat taccgttcgt cgggcaatta ccgatgatat cgccagcctt
    15421 tctgcagtat cgcaggtgga ttatgacggc atcatcctta agcttaatca gctttcaaat
    15481 caggtagata acctgcgtct ggccgataat gacagcgatg gttcgccgat ggattccgac
    15541 ggtgaagagc tttccagttc catcagcgaa tggcgtatca atctgcaaaa aagctggcag
    15601 aactttatga acaacttcat tacgattcgc cgtcgtgatg acaccgccgt accgctgtta
    15661 gcgccaaatc aggatatcta tctgcgcgaa aatattcgct ctcgcctgct ggtcgcagca
    15721 caagctgtac cgcgtcacca gcaagagact tatcgccagg cgctggagaa cgtctccacc
    15781 tgggtacgtg cttactacga tactgatgat gccaccacca aagcgttcct cgacgaggtg
    15841 gaccagttaa gccagcaaaa tatctcgatg gatcttccgg aaaccctgca aagccaggcg
    15901 atgctggaaa aactgatgca gactcgcgtg cgtaacctgc tggcacaacc ggcagcgggg
    15961 acaacggaag ctaaacctgc acctgcaccg caagctgcac cgcaagctga tactccggca
    16021 gccgcgccgc aaggagaata accatgctaa aagtgttatt gctctttgtg ttgctgattg
    16081 cggggatcgt ggttggcccg atgattgccg gtcatcaggg ttatgtgctg atccagaccg
    16141 acaactacaa tatcgaaacc agcgtcacgg gcctggcgat catattgatt ctggcgatgg
    16201 tagtgctgtt tgccattgag tggctactgc ggcggatctt ccgcactggc gcgcacaccc
    16261 gtgggtggtt tgtcgggcgt aagcgtcgcc gtgcacgtaa gcagaccgaa caggcgctgc
    16321 tgaaactggc ggaaggcgat tatcagcaag ttgaaaagct gatggcgaaa aatgccgatc
    16381 acgctgaaca accggtggtg aactatctac tggctgccga agccgcgcaa caacgtggtg
    16441 atgaagcgcg cgccaaccaa catctggaac gcgcagcgga gctggccggc aatgacacca
    16501 ttccggtaga aatcacccgt gtacgtctgc aactggcccg taatgaaaac catgctgcac
    16561 gccacggcgt ggataagctg ctggaagtta cgccacgcca tccggaagta ttacgtctgg
    16621 cggaacaggc gtatatccgc acaggtgcat ggagttcgct gctggatatt atcccatcaa
    16681 tggcgaaagc ccatgttggt gatgaagaac atcgtgcaat gctggaacaa caggcatgga
    16741 ttggcctgat ggatcaggcg cgtgccgata acggtagcga aggtttgcgt aactggtgga
    16801 aaaaccaaag ccggaaaacg cgtcatcagg tagcgttgca ggtggcaatg gcggaacatc
    16861 ttattgaatg tgacgatcat gatactgccc agcaaattat catcgatggc ctgaaacgcc
    16921 agtacgacga tcgcctactg ctgccgattc ctcgactgaa aacaaacaat ccggaacagc
    16981 tggaaaaagt gctgcgccag caaatcaaaa acgtcggcga tcgcccgctg ttgtggagca
    17041 cactgggcca gtcactgatg aagcacggag aatggcagga agcatcgctc gccttccgcg
    17101 cagcgctgaa acaacgtccg gacgcctacg attacgcatg gcttgccgac gcgctggaca
    17161 gactgcacaa gccggaagaa gctgcagcga tgcgtcgcga cggtttgatg ttaacgttgc
    17221 agaacaaccc accacagtag ttccttctca cccggaggca agcacctccg gggccttcct
    17281 gacacataaa aaaacgcctg ctcttattac ggagcaggcg ttaaaacagg tctgtatgac
    17341 aacaagtggg tgcttcactc aacgttgtgt ccatggtgtc tgatgaggca taagcgacat
    17401 ctgtcagtgg acgataagca ccgtaaacgg ctctgcgtca ttccggagtt tatgaggcac
    17461 taaggcgaac ataagagatg gaatgagcat ctactcgttt attatgccac agagaatcgg
    17521 gaaataacat cccttaacac ttgttatgag ataattctgt aatcctcttg gcttcctgag
    17581 taataacttc ctgagtgaat atttaccctg agcttgattc tacacatact cattatgaat
    17641 gataaaattc attcaattaa taacacatat attaattgcc gttaaaacta aaaacagcat
    17701 caataatcaa cgcgatataa taaacctgcc ttacatatca actgcgccag aggtaggatt
    17761 gaaaacgctc tcctgatttt ccaattcatt ttctggataa ataaataatt tatttttgtc
    17821 actattattt atgtaatcat cctgtcaggg agagggatct caattatcaa tgcttaatta
    17881 cgtcatcatt ttgatgacat gaaaccggag cattttgcat ggaattttcg ttttcaccca
    17941 aacgtcttgt tgttgctgtc gccgccgctc ttcctctcat ggccagcgca gcagataccc
    18001 cgtcaactgc caccgcacgc aaaggctttg ccggatacga tcacccaaac cagtatctgg
    18061 ttaaaccggc gaccactatt gccgacaaca tgatgccagt aatgcagcat ccggcgcagg
    18121 ataaagaaac ccagcagaag ctggcagaac ttgagaaaaa aaccggtaag aaaccgaatg
    18181 tggttgtttt cttgctggac gatgtgggct ggatggacgt cggttttaac ggtggcggcg
    18241 tggcggtggg taaccctaca ccagatatcg acgccgttgc cagccagggg ctgattttaa
    18301 cttcggcgta ttctcaacca agctcttccc caacccgcgc caccattctc accggacaat
    18361 actccatcca ccacggcatt ctgatgccgc caatgtacgg gcaaccgggc gggctgcaag
    18421 ggttaaccac gctgccacag ttgctgcacg atcagggcta cgtcactcag gccatcggga
    18481 aatggcatat gggggaaaac aaagagtcgc agccgcagaa cgttggcttt gatgatttcc
    18541 gtggctttaa ctcggtgtct gatatgtaca ccgaatggcg cgacgttcac gtcaatccgg
    18601 aagtggccct gagtccggac cgttctgaat acatcaagca attaccgttc agcaaagatg
    18661 acgttcatgc ggtgcgcggc ggcgaacaac aggccattgc cgatattacg ccgaaatata
    18721 tggaagatct ggatcaacgc tggatggact atggcgttaa gttcctcgac aagatggcga
    18781 agagtgataa gcctttcttc ctctactacg gcactcgtgg ctgccacttc gataactacc
    18841 caaatgcgaa atatgcaggt agctctccgg cacgcacctc gtatggcgac tgcatggtgg
    18901 aaatgaacga tgtgttcgct aatctgtata aagcactgga gaaaaacggt cagcttgata
    18961 acacgctgat tgtctttacc tccgataacg gaccggaagc cgaagtaccg ccgcacggac
    19021 gcacaccgtt ccgtggtgcg aaaggatcta cctgggaagg cggcgttcgc gtaccgactt
    19081 tcgtgtactg gaaaggcatg atccaaccgc gtaaatctga cggtattgtc gatctggcag
    19141 atctcttccc taccgcgctg gatctggcag ggcatcctgg cgcgaaagtg gcgaatttag
    19201 tgccgaaaac cacctttatc gatggtgtgg accagacatc tttcttcctg ggaacaaatg
    19261 gtcagtctaa ccgtaaggcc gagcactact tcctcaacgg taaactcgct gctgtgcgta
    19321 tggatgaatt caagtatcac gtcctgattc agcaacctta cgcttatacc cagagcggat
    19381 atcagggtgg attcaccggc acagtaatgc aaacggcggg atcgtcggtg tttaacctgt
    19441 acaccgatcc gcaggaaagc gactccattg gcgtgcgcca tattccgatg ggtgtaccgc
    19501 tacagaccga aatgcacgcg tatatggaga tcctgaaaaa gtatccacca cgcgcgcaga
    19561 ttaaatctga ttaagccggc gctatcgctg agatctgcct ttgccggatg cgatgctgac
    19621 gcatcttatc cagcctacag aacgctgcaa tttattgaat ttgcacgatc atgtaggccg
    19681 gataaggcgt ttacgccgca tccggcaatc aaccgcaggc ggccacaaaa tggccgcttt
    19741 tttacttact caccaccagc aaatgcgcat gcataatgtc gctggccgga cgaccgtgcg
    19801 ccagcaaatc agccatagct ttaagatatg gcggtagatg gcggaaataa cgctgatacc
    19861 cggcacacaa ataattcagc cccggtttgc cgctggcatc gagcatgaag cggtgtttcg
    19921 gacagcctcc ccaacatgct tttaacacgt tacaactgcg acactgcgcc ggtaactgct
    19981 taaatttatc ttcaccaaaa gcctgctgct gcggagaatc taccatttct gcaattgttt
    20041 gctggtgcat attccccagt cgatattgcg gataaacata gtgatcgcag gcgtaaacgt
    20101 cgccgttgtg ctcaacaatc accgagcgcc cacaggttgg ctgatgatgg caaaccgcac
    20161 ccggcgcacc gacaaaattg gcaaacgccc attcgatatt catcacaaaa atcttgccga
    20221 catcacgttt gatccagtgg tcgaaaatcg ccaccagaaa ttcaccaaat tcatcggggc
    20281 gcaccgacca ttccgttagc tcaccctgaa tatcaccagg cgcatgcaat ttcagtcctt
    20341 cgcgggcagc cgtttcatca gccaagcgct cgaccaccgg aataaactgg atgaattcca
    20401 ctcccgcatc gcacaaaaaa tcatatacct gcaacggttg ctgcgcgctg gtgcgattaa
    20461 cgcagaccag cacgttatag tcgacatgat gtttttgcag cagcgtcagg gcacgcatca
    20521 ccagcttatg cgttggtctg ccacctttgg tcacgcgata ttgattgtgg atctcagccg
    20581 gaccatccag cgataaccca acaagaaaat gattttctgc cagaaatgca caccattcgt
    20641 catcgagcag cacgccgtta gtctggaagc tgttacttat cttcctgcca gcaccatatt
    20701 tcgcctgtag cgctacggca cggcggtaaa actccagccc gagtagcgtt ggttcgccgc
    20761 cctgccaggt aaaagccact tcgttttgcg gttcgctggc agcgatatag taacggacat
    20821 acgcttccag cgtgtcatcg tccatatgcg tgactggttt ttcgcggtaa agggattgtt
    20881 tttcgagata aaaacagtag tcacagttca gattacaatc ggaaccgctc ggtttcgcca
    20941 tcacatgaaa agcacgcgtt ggaacctgtt gcagcatggt cgctccttgc ctgaagtcat
    21001 ccttcaatac gttaagggcg aaatcatacc gtggggcaat gagttattac gggcgtttat
    21061 tgcgaaggaa gggtgataga atgcacagaa aacaaaaaac cccgccgaag cggggttcaa
    21121 aattggtcgg cgagagagga ttcgaacctc cgacccactg gtcccaaacc agttgcgcta
    21181 ccaagctgcg ctactcgccg aaatactgct ttttgaattt ttagttcaat tctttaaagt
    21241 cgtggtgcga ggggggggac ttgaaccccc acgtccgtaa ggacactaac acctgaagct
    21301 agcgcgtcta ccaattccgc caccttcgca ttgtcacaac ttctaataat ggggtggcta
    21361 atgggattcg aacccacgac aactggaatc acaatccagg gctctaccaa ctgagctata
    21421 gccaccacta caaatcttgt tacgcggtat tactaccacc gcagctcaag cgccgggact
    21481 aaatggcgcg cccgacagga ttcgaacctg agacctctgc ctccggaggg cagcgctcta
    21541 tccagctgag ctacgggcgc ttagcgccgt tgcgggggtg gataatacgg acttcccacc
    21601 cctctgtcta gtcccttttt aaataaattg cgcgtttggt tacggtttgt gcgttttgct
    21661 gcttattcct ccagtttatg cgctttcccg tggcgattaa ggccaaaaac tttataaatc
    21721 gccgtcaccg ccagcataaa gatgatgcca acaaacagcg acatacgcgt atcttcatta
    21781 aagtacatgc cgatcaaaac gcaaatcagg aatgccattg ttacgtagtt ggcccacggg
    21841 aacaggattg agcggaacgg atggctggca atcgccgctt tatgtgcacg ccgaaaacgc
    21901 agctggctta tcaatatcac aaaccatggc accatccccg gaagcacgct ggcactgtag
    21961 acgtagacaa acacacgctg cggattggga atgatgtagt tcaggcatga gccaattagc
    22021 agaatagcaa tagatactgc cacacccgca acgggtacgc cgtgacggga aactttcgcc
    22081 atcgccgccg gtaactgacg gtttttcgcc agtgcgtaga gcatacgtcc gcaactgtac
    22141 atgccgctgt tacagccaga gagcgcagcc gtcagcacca caaagttgat aatgcccgcc
    22201 gctgcggtaa taccgatttt ggcaaaagtc agtacgaacg ggctgccgtt gctgcctatt
    22261 tcattccacg ggaagatggt gacgataacg aaaatcgcgc ctacgtagaa aatcaggatc
    22321 cgccacagca ccttgcctac ggcactgcgc agcgtcacct gcggattctt cgcttcaccg
    22381 gcagtaatgc caatcagctc cacgccctgg taggacgcca ccacaataca cagcgcggtc
    22441 aggaaccctt tccagccacc cgcaaagaaa ccgccatgct ctgtgagatt gctaaaacca
    22501 atcgactgcc cgccattgcc aaagccaaag aaaatcacgc ccaggccaat gacaatcatc
    22561 acgataatcg tggtgacttt gatcatcgcg aaccagaact cgatttcgcc gtacaaccgc
    22621 accgccgcca gattcgccaa cgccaccagc gccactgcga tcaatgcggg tatccactgc
    22681 gccatctccg ggaaccagaa ctggacataa acgccaatgg cggtgatttc agagatcccc
    22741 accgccatcc acataaacca gtaagaccag gcggtgagat agccaaagaa cgggctcata
    22801 taacgatgcg cataaacggc gaacgaaccg gtaaccggtt cgaggaacaa catttcgccc
    22861 attgaacgca tgatgaaaaa gacgaacagc ccggcgatga tataggccaa caatacggat
    22921 ggcccggccc atttcagggt actggcggcc cccataaaca ggccgacgcc aatggtgccc
    22981 cccagggcga tgagttcgat atgtcgagct tccagcccac gctgtagctc tggtttgtta
    23041 tctgccataa atcctcgtgt tgtgtttgca tgctttccgg tgttaccggt tatcgttatg
    23101 ggtacatcga gtgttgcgaa tgttttcgta attcaggaga aatggcaaat aaagcattaa
    23161 aaatttgaat gctttgtgta ataaaaaagc agacaggcga cgaagtgacc actccgtcgc
    23221 tttacaaaga gaggaaaatc agaggttgcc ggtgtaatgc cagcgtaaat aacgcagcaa
    23281 acgaagctga cgcttaatgc ggctcggctg cgaaagcagg cggtagagcc actccagccc
    23341 cagcgtttgc cagattttcg gtgcgcgttt cacgtgaccg gtgaaaacat cgtaagtccc
    23401 gccaacgccc atatacagcg catctggatg caccagacgg cagtcgcgca tgaagatctc
    23461 ctgctttggc gatcccatcg caacggtgac gatttgcgct ccgctggcat gaatgcgttc
    23521 aaacagcgcc tgacgctgct cgggtttaaa ataaccatcc tgactgccaa cgatattcac
    23581 attccattgg ttgcgcagtt tagcttcagt ttgcgccagc acttcaggtt taccgcccac
    23641 aagaaatact ggcgtccctt ctttgcctgc gcgcgccatc agctcttccc aaagatcggc
    23701 accggcaacg cgggaaacct gcgcctgcgg gtacttttta cgtactgaac gtacaacgct
    23761 gatgccatcc gcatatttaa attcggcagc gttaattaac tccctgacct cggcgttatc
    23821 ttcaatggtc agcatttttt cggcattaat ggcaaccaac gttccctgct taagctgccc
    23881 gtcagcaaac agataatcga gggcgtgctg catatcacgc caaccgatca actgtaagcc
    23941 acgcagcgta taggtcggtg ccgtggtgtt gttattcatt gttatccttc aacctgcgtc
    24001 cggagcgatg attttgtacg tttatgaatg agtccggcgc tttcaaaaag ccagtacaac
    24061 agttttgcga tcatcagaca tgcgccgaag accacgataa aaaagactac gcgtgagaca
    24121 aacgaatcca gcccttcacg cgccagcacg atcatattga agatcgcccc aaagcagaaa
    24181 ctgtgcaata tcgcagcctt atagcgatta gtctcgcggt tgcccagctc atacagccag
    24241 tcgaaccatt tgatgatcag tccaaccaca atcgccccga gcgggatgaa caacgcgccg
    24301 cccatcacca ccagtgagcc tataagcgta ggcgagatcg ccagtccgga gtggttattc
    24361 agcacttccc aggtaaagta gttggctgag ttcagcacca tactcgggcg acccggccac
    24421 agccaggaag ggataaagac atagaaatcg cggacaattg gagccaggcc ctggaagtcg
    24481 atgttgtcgt agttctgcaa cagcaacgcc agattctccc acggcgagaa ggtgtcgcga
    24541 gtgagataga gaaacgtata gaacgcttca tcgccgctca cattcattcc atagcgtttt
    24601 agcgccagcc agaacatgcc aacaatcccc agcacgcccg ccgccgccag catccacaac
    24661 gaaatccagc cgcgaataat gccaataaac aggaagatag cgaatgcgat gatgatattg
    24721 gcgcgagtgc cgccgacaat catataagtc agcaagccaa aggcgaccgt gctgacgagg
    24781 aaaaacagcc acgctttgct gtcctggcgc agaaagtaga ccaccagcat cgccgggatg
    24841 aaaaagtaaa agaagcgttt taacgccacg ccggagactt cactggagaa gatctgactg
    24901 taggagttaa gccggaacag caaaaagccg ttgtgcatga agaagatgcc gacgcttacc
    24961 agcgcgatgc ctatcaggat cacccacgta agattggttt ccacgcggtt catggtaaac
    25021 agcggacggc gcggtacatc agcaacgcgt ttgcgtaggc gggttttgta ggtgacgtag
    25081 taaaccgcgt agaagcagcc cgcagaaagc aacgcctgca acaagatttc tggcggcgcg
    25141 acaccaacat caaagcgaaa taccagcacg ctggtcagcg ggaagccgaa gaaaaaggtg
    25201 agcaaaaaca gcaatgaaaa gaagacattg aagttaaagc gcacacggcg aaactcaaac
    25261 caggtcagcg tggcaataaa cagcgtgcag agcagccaga caacaaacag gccactgaat
    25321 tgcagcagac tcatgcgacc tccccggcgg caatcgccaa cgcccgctgc cagccttgca
    25381 gatagttcgg gctaaagaag gcaatggtgt ttttatccac cgacgccaac tggcgctgcg
    25441 cttcacgcac aatatcctcg ttgagatcgt cggtagtaaa cagcaccggc aaatgctgtt
    25501 ccgtcatatc ctgccagaac ggattttccc ggttaagcac acaaggaatg cccgcctgaa
    25561 tcagtaagca cagcgtacca atgccctgct ggcgggcaaa aataaagtaa ccgagatcgc
    25621 actgacgaag tagcgccaga taggcgtcaa attccagttt ttcgctcaga acttgcagat
    25681 tttcttcgct gaataactcc agcccagcct gacgaacttc ctcaatgtac gcttcgttat
    25741 taggcggata tcccatcggc accaccactt ttaccgtatc gccaaattgc tgatgaacgg
    25801 cgcgcaaggc agcaatatgc tcattgctgc ggtcgccgga gttccccacc agaatggtca
    25861 ttttcccttc acgttgccga tcgttcgcca tcgtattgag agaagagtcc atccgcgtcg
    25921 ggaagtacag cagttcgccc cgcacctttg gatgcgtttt ggcaaaaaag ctcaaatcac
    25981 cgcgggtggc aaatacacag ccgactcgct tttgcgccag gcgacgtagc gggtaaaaaa
    26041 gcttatatct caagccactg gaaagctcat acagatctgc cccccagata tgccagaaaa
    26101 actggcggga cttaatgcca ccactcagca aagccagcca cagtttggga ttgaactgac
    26161 cgtggaagaa aaaacgctgc tgacggttag cttttgcttt cgcgatgacc gcttccgcca
    26221 gcgatttttt cccagggaaa aattgcacag aaagcgccgg acagttatca cttaagccgt
    26281 cgtccttgcc aacaaccata aactcgcgcg cgtgctcgct cgtcgcggcc agcgcgtcat
    26341 tgaaaaaccg caaaacggtt cggttatggt gagggatatc cgatcccagt acgtgaatca
    26401 gtacagtcat gcccgcctac gccagagtaa aaacacgcca caacaaagag aaaaatagac
    26461 gatataagtt gccatatagg cctgcgccgc gcccagtgca ccatgcgcag ggattagcca
    26521 gtgggcaaat accatcaata aagtgaactg gctgacttcc gccagaatat aaaaccgcag
    26581 tgaagctttg gcgatcacca gataaccaaa gacataagcg cccactttta acacatcacc
    26641 cactaactgc caggcaaaga gatcgcgcat agcggtaaat ttattcgaca acagcagcca
    26701 gatagcaaaa tcacgcagca accagacggt aaaactcgcc gccgccactg ccggtaagac
    26761 gaatttcagc gatttaacca cttcccgggt gatatcgcgc ttttccgtta gccgcgacaa
    26821 cgtgggcagc aaatatacgc tgaacgatgc cgtaataaat tgcaggtagg catcggaaat
    26881 actgctcacc ccttgccaga tccccacttc atcccagcta tactgcgccg ccagcagttt
    26941 acgcatcatg atgtaagcaa caggcaaggt caccgacgta atcaacgcca tgagcgtaaa
    27001 tttgctcaac tgccctgcca gaccgttatc ccagctgggt ttcagatagc ttaacgggat
    27061 gacaccacgt ttgatcaaca tgatggcggc aggaatcacc accagcgcgg gaatcagcgc
    27121 cagacccagc aacgcccctt cataaccgcc caaacggtaa ctgacgtagt acgcgagaac
    27181 gccaatcaag ctgccaacaa tcagcgataa cgcattacct gcggcatcgc gaaagccttt
    27241 catcagcgcc agtaacaggt tgccccaggc gatccccatt tgcaccagcg ccactaaacg
    27301 caccagcccc tgatagtcgg tattaccaaa caatccctgg ctgattggcg cagctgccag
    27361 cacaaaaacc agcgccatca gcgtagagaa gccaattacc atcgctgatg aagtgccgac
    27421 cacgcggcgc agctgttggg gattatcatg gtactgggca acgtatttgg ttacaccgtt
    27481 aaagatgcca gccccggcaa gcacgccgag cacggtaatc aactggcgga aatttgccgc
    27541 cagcccaagc cccgccggac caaatgacac cgccagcaac ttaccgacca gtaacccggc
    27601 accaatcttg accagtgtac tggccgccgt ccacaaggac gcttttgcca acgacatatc
    27661 aggagaagta gttcaacaaa gtcgcaatta ccgtacgctg attgacgggc gacaggttgt
    27721 agaacagcgg caggcgcagc aggcgctcgc tctctttggt ggtgtagcga tcttcaccgt
    27781 ggaactcacc aaagcgttcc cccgcagggc aaccgtgcag cggaatgtaa tggaacaccg
    27841 ccatgatttc cgcttctttc agaaagttaa tcaacgcgct ccggtcatca atatcccgca
    27901 gtttaatgta gaacatatgc gcgttctgca cgcagccatc gggaatcgac ggcagctcga
    27961 tacgcccggc tttcgccaga ggtgctaacg catcgtagta gttttgccac agcgccagac
    28021 gttgctggtt gatacgatcc gctgcttcca gttgcgccca caggtacgca gcttgcagat
    28081 cggacatcaa atagctggag ccaatatcgc gccaggtata tttatcgacc tgaccacgga
    28141 agaactggct gcggttagtg cccttttcac ggatgatctc ggctcgttcg attaacgctt
    28201 tatcgttaat cagcgtcgcg ccgccttcac cacccgccgt gtagtttttg gtttcatgga
    28261 agctaaagca gccaatatga ccaatggttc caagcgcacg ccctttgtaa gtggacatca
    28321 cgccctgagc ggcatcttct accacaaaca gattatgctt tttcgccaac gccataatgg
    28381 tgtccatttc gcaggccaca cccgcgtaat ggaccggcac gataacgcgc gttttgtcgg
    28441 tgatcgccgc ttcaatcagc gtttcgtcga tgttcatggt gtccgggcga acatccacaa
    28501 agacgatttt cgcgccacgc agcacaaagg cattggcggt ggagacaaag gtgtagctcg
    28561 gcatgatcac ttcatcgcca ggctggatat cgagcagcag cgccgccatc tccagcgaag
    28621 cggtgcagga cggcgtcagt aacactttgg cgctgccaaa acgttgctcc agccactgct
    28681 ggcagcgacg ggtaaaaccg ccatcgccac acagtttgcc gctacccatt gccgactgca
    28741 tatagtcgag ttcggttccc accaccggcg gtgcgttaaa tggaatcatg tgatcacctg
    28801 tataaccagt acgcggtgct ttctacattc gcaccacttt gtatgtatcg tttaagcgcg
    28861 gcggtgttgc ccatttgggt cgccacccgc aaagttgttt taccgcgagc atacgcccag
    28921 tttagcgccg tttgcatcag ctcagcacct gcaccgcgtc cagccagcag gcctattcgc
    28981 gcatctgtcg cattgagttc ccgtaaagag acatagccgc gaatatcgcc ggacgccgca
    29041 cgtaaaatca gacattgatg atcaaaggtg ccgcgcacgg cattttcaat ccactgtgca
    29101 taaaagcgac tgctggcgtc aggcgcatac cacggcgcac gaaaacggct ttgcgcaaat
    29161 gcggcgctgg ctaactgacg taatgcggga atatcggtct cttgtgccac tacagcaccg
    29221 ctatcactgg cattgttcac gggtagcgcc aaatcaactt caccttctac cagggagaat
    29281 cccagctgtt gcagggcatc cagttcaccc gtatttgatg ccgcaatttt ggcctgcacc
    29341 cgtgaccacg gcgctaacgc gtctggcgtc aggagcggtg cttcagacgt aatgcgcacg
    29401 atggcgctgt taacaccaaa gaaggcgttt tcccaggtta gtggctcaat actggcgcgg
    29461 acgggcacga agtaactcca gcagatattg gccgtagcca gttttcgcta atgaactggc
    29521 agcacgcttc acaccctcgt catcgagcca gccgttacgc caggcgatct cttccaggca
    29581 ggcaatctta aagccctggc gtttttccac cgtctgtaca aaggtgctgg cttcaatcag
    29641 gctgtcgtga gtgccagtgt ccagccaagc aaatccgcgc ccgagcagtt caacggtcag
    29701 gttgcccgcc tcgaggtaca tctggttgat ggaggtaatc tccagttcac cacgctccga
    29761 cggcttcacc tgctttgcgt actccacgac tttactgtcg tagaaataaa gcccggtcac
    29821 cgcccagttt gacttcggct gttttggctt ttcttccagc gagatagcgc ggaaattgtc
    29881 gtcaaactcc accacgccaa agcgttccgg gtccatcacc tgatagccaa aaaccgtcgc
    29941 cccttccgtg cgcgccgcaa catgacgcag cttcggactg aagccctgac cgaagaagat
    30001 gttatcgccc agcaccagac aagaaggttc accattaagg aaggtttcac cgatgataaa
    30061 ggcctgcgcc agaccgtccg ggctgggctg ttcggcatat tccagctgga taccgaactc
    30121 actaccatcg cccagcaggc gctggaaata acctttatct tccggcgtag tgatgatgag
    30181 aatttcgcgg ataccggcca gcatcagcac cgacagcgga tagtaaatca ttggcttatc
    30241 gtaaatcggc aacagttgct tcgatacgcc gcgcgtaatc ggatgcaatc gggtgccgga
    30301 accgcccgcc aggataatac ctttcatgtt tgcctccgct gaaaattagc ctttcagacc
    30361 taaacgctcg ccctgatagc tgccgtcctg cacctgcttc caccaacttt cattagccag
    30421 ataccactgc accgttttac gcattccact ttcaaaggtt tcctgcggca gccagccaag
    30481 ttcacgggca attttcgaag catcaatggc atagcgcaga tcatgccccg gacggtcagc
    30541 gacaaaggtg atcaagtcac gataatgcac cacgccttgc ggcttgttcg gagccagttc
    30601 ttccagcagc tcgcaaatgg tttccacaac atcgagattc ttacgctcgt tgtgaccacc
    30661 aatattatag gtttcaccga ctttcccggt ggtcgccacg caatacagcg cgcgggcgtg
    30721 atcttccaca tacagccagt cacggatttg ctgcccgttg ccatataccg gcagcgattt
    30781 acccgccagc gcgttgagga tcatcagcgg gatcagtttt tccggaaagt ggtaagggcc
    30841 gtagttattc gagcagttgg tgataagcgt cggcagacca taggtccgca gccaggcgcg
    30901 caccagatgg tcgctgctgg cttttgacgc ggaataaggg ctgctcggcg catacggcgt
    30961 ggtttcggtg aagaaatcat ccgtcgagtg caggtcacca tatacttcgt cagtggagat
    31021 atgatgaaaa cggaacgctg attttttatc ttccgttagc gcattccagt aagcccgcgc
    31081 cgcttcaagc aatgtataag tcccgacaat gttggtttca ataaacgctg ccgggccgtc
    31141 aatagaacgg tcaacatggc tttcggctgc cagatgcatg acacagtctg gctgatgctc
    31201 agtgaatacg cgcgccagtt ctgcccgatc gcagatatca actttctcaa aggcaaagcg
    31261 ctcgctttgc gcgaccggtg ccagcgacat caggtttccg gcgtaggtca gcttatcgac
    31321 cactaccacc gcgtcgctcg tttcgttgat gatataacgc accagcgccg agccaataaa
    31381 cccggcacca cctgttatca gaatctttct catcagcgcc agactccttt ggcatcgacg
    31441 acatactgct gatggacatt gtcgccattg ataactttga actgactatg atcgaccagc
    31501 atcaccagca cgtctgccgt tgccagcgcc tcgtcaagct gcgccagagt acaaagcccg
    31561 gtcagttttt tcggcaactg gtggatgtta ggctcaacaa ccagagtttc gccgctatgc
    31621 cactgggcga tcagttcagc gatttccatc gccgggcttt cgcgcaggtc atcaatattc
    31681 ggtttaaacg ccagaccaaa gcaggcgatt ttcagttcac tggcgcgttt atcggtagcc
    31741 gccaggcaat cagccaccgc cgctttcacc tgatcgataa cccagaacgg tttgtgatcg
    31801 ttcacttcgc gcgcggtacg gataagccgc gcctgctggg ggttctgtgc cacgataaac
    31861 cacggatcaa cagcaatgca gtgaccgccc acgccagggc caggctgaag aatattaacg
    31921 cgagggtgac gattcgccag gcgaatcagt tcccagacgt taatcccctg atcggcacaa
    31981 atcagcgaca attcattagc aaaagcgata ttcacatcgc ggaagctgtt ttcggtgagc
    32041 ttacacattt ccgccgtccg cgagttagtg acgacacact caccttcgag gaaaattttg
    32101 tacagttcgc tggcgcgggc cgaacaaacc ggcgtcatac caccaatcac gcgatcgttt
    32161 ttaatcagct cgaccataac ctggcccggt aacacgcgtt ccgggcagta agcaatgttg
    32221 acgtccgcct gctcgcccac ctgctgtggg aaagtgagat ccggacgcat ctctgctaac
    32281 cattctgcca tcttctcggt tgaccccacc ggcgaggtgg attcaaggat caccagcgcg
    32341 ccttttttca gcactggcgc aatggagcga gcagccgatt caacgtaggt catatctggc
    32401 tcatgatcgc ctttaaacgg cgtgggtaca gcaatcagcc aggcatccgc ttcaactggc
    32461 gtcgtgctcg ctcgtaaaaa accgccttct acggcagttt ttactacgct cgccaaatca
    32521 ggctcgacga tatggatttc gccacgattg atagtatcaa ccgcgtgttg gttgatatcg
    32581 acaccaatca cctgtttttg ccgtgaggca aacgctgctg ctgtaggcag cccgatgtaa
    32641 cccagtccga taacagaaat ggtcgcaaaa ctcatagtga tatccgatta ttttttaacg
    32701 cttccagaat gcgagagcat gcctgaccat caccatacgg gttatgggcg cggctcatag
    32761 tttgatattc gttttcgtct tttaaaagac gcgtcacttc ctcgacaatt cgctgcttat
    32821 ccgtgcctac cagacgcacc gtacccgccg tcaccgcttc cggacgctca gtggtatcgc
    32881 gcatcaccag caccggtttc cccagcgaag gcgcttcttc ctgaatgccg cctgagtcgg
    32941 tcaaaatcag ccaggcgtgg ttcatcagcc agacaaacgg taaatactcc tggggatcga
    33001 tcagaatgac atttttcaca tgccccagaa tgcgattgac cggctctctg acgttcgggt
    33061 tgagatgcac cggatagaca atctggatgt cctggtgcgt ggtggcgatg tctgccagcg
    33121 cgtggcagat ttcttcaaag ccacgaccga agctttcacg cctgtgaccg gtcaccagaa
    33181 tcatcttttt atcggggtcg ataaacgggt aatttgccgc aagttctgaa cgcagcgtgt
    33241 cgctgctcat cacctggtca cgcacccata acagtgcatc aatgactgta ttaccggtaa
    33301 tgaagattcg gctatctgca acgttttcac gcagcaagtt ttgccgggaa gtttcggttg
    33361 gagagaaatg atacatcgcc agatgcccgg tcaatgtacg gttagcctct tccggccacg
    33421 gcgaatagag atcgcccgtg cgtaaaccag cctcaacgtg accaacagga atacgctgat
    33481 aaaacgccgc caggctggtt gccagcgtcg tcgttgtgtc gccgtgaacc agcacgacgt
    33541 ctggtttgaa ctcggcaaga ataggtttta gcccttccag aatccgacag gttatctctg
    33601 tcaggccctg tcctggctgc attatgttga gatcgtagtc aggtacaatg gaaaagagtt
    33661 tcagcacctg atcgagcatc tcccgatgct gcgcagtgac gcaaacttta gcctcaaaaa
    33721 aaggatcttt tgccaacgca tgcaccaacg gagccatctt gatggcttcc gggcgcgtac
    33781 caaatacagt cagtactttc acatcgattc tcttcgaata agcggcgagc gactttgcgc
    33841 tcaccgcagc agtgttgcta tttcgagcaa cggcgggtta atgcgacacc agccccgatc
    33901 agccccccga caatgcccca cataatcatc aggaaggcac gacgtgggct atcgcgtttt
    33961 accggttctt ccggcgtacg caaatagcga taggtctgaa aacgcggatc cagggttgga
    34021 ccaacattca gggtgtttaa catggcccga ttctgatcat agtcgagatc aaaggccgga
    34081 ccgacggcct gtaaattttc cagtcgagcc tggagcattg gacgcccaag caggaacatt
    34141 tctgaatcag gtaattcctc ggcaggcaca tctgtcgcac tgcgcgaaat attatgctgc
    34201 tcagcaattt tcagcgcctg ctcaatgctg ttcatccggc ggtcgtagat ggctttcgcc
    34261 acctcttcct gacgcttcac ctgagctttc atctggatgg tgcgtgccgc ccatgcgcct
    34321 ttcagctcat cattcagatg gctggctgca cgctggctgg caaaagcaac atactgacgt
    34381 aacaggttat tagcgtcagg cgcggtttcg gcaataagct tcacgctgtc attgaccgcg
    34441 cgggtaaagt ctccggggat aaactggatg ttgttaatca tttcatccag caacgccgca
    34501 tcggctttgc tgttgcccac catccgctgc ttgtaatagt cggtttgcag ccagaactct
    34561 ctgcgggtat cccacgaggc cagttgcata acaaactctt tataggcttc gtccatgacc
    34621 gatggttggt cggcagaagc catgtttgaa cggacatcca ggttacgcaa aaattgctgc
    34681 tgcgagtaat atccccccag catattcacc gttggacgat cggtaatcgc cgtcgagctc
    34741 cactcctgac gagcaaaaaa cgtatacgcc agcgcgatta acgcaaacgc cagccccatg
    34801 ccaataatcc atagcttccc agcccacaag gtacgaaaca acccacgaat atccagttca
    34861 ttttcagcgt cttcggccgg tttcccaggc attggttgtg tcatcacatc ctcatttatt
    34921 tggttaaatt ggggctgcca ccacgatttc tacgcagtct gcgttttacg cgcttaataa
    34981 agcgagcaac tttccaggca cgcttaatgc aatatccata gaggaagaat gctagcaaaa
    35041 agagcaccag catgacccac tccgggacaa aatgagaata ttctgccagc acgccaatgg
    35101 aagcgagcag tgctgcggca agggtaatca gcacaaacgc ctgacgggaa gtaaacccgg
    35161 cacgcatgat caaatggtga atatgctgcc ggtcaggaga gaatgggctc atgcctttac
    35221 gcaggcgacg gtacataatc gccaccatat ccattagcgg aatggcgatt atccacaaag
    35281 cggtaaccgg gctgatggga tgggttttgc cctgggtcgt ttcgagcagg atccagatca
    35341 cggtaaaacc aatcagcgta ctgcccgcat cacccataaa gactttgtag cggcgaccca
    35401 ggataccaag gttaagcatg atgtatggca ggatggcggc gatcatcgca aagcaccaga
    35461 ttgcgaggct ggtttgcccg tcgaaccaca aaatcatacc gattgctgca aacgagacac
    35521 aggacaaccc gcccagcaag ccatcaatgc catcaaccat gttgaacgca ttaatggccg
    35581 cccagacggc aaatagcgtc aggaagtaac caaacggtcc gagcaccatc tcccaggagc
    35641 caaagatata acccaggcta ctgagataaa gcttgccgaa caccatcata acaatgccaa
    35701 cagcggcctg tatggtggca cggattttta cgctgatatc aaaacggtca tccagcgcgc
    35761 caatgaaaac aagcacaccg gcacaagcga gatagagaga tgcatgcgga atatagtaat
    35821 cgacaattcc gaacgtgaag caaatccctg cgtaaaccga aatcccccca acgagaggta
    35881 tcaatccctg gtgacgtttg cggaagtttg gtttatccac taaaccgact ttttttgcca
    35941 ccttacgggc aaaaaacaga aacagtgtcg tgaataaaaa aatactgatg agatcagtac
    36001 tcactgtcag taaattcaca atgcatgctc tcagagaaaa ttattagcag aagtataacc
    36061 acgaagacct ttattcagaa gggaaagtcc gttccgaacc caccagtcct gtgcaaaaat
    36121 taaccatttg atatgcaaat tgcataacat gccagcaaat tccaggaatt ttcctaacat
    36181 cgccagcgcg gcattaagat tacagatata aaagcaaaac gccacgtaaa cacgtggcgt
    36241 ttttggcata agacaaattt atgagcgttt catcatttcg aagaaatcgt cattggtctt
    36301 ggtcattgcc agtttattaa tgaggaattc cattgcatcg atttcgccca tcgggtgaat
    36361 gattttgcgc aggatccaca ttttctgcag ttcttcctga gtcgtgagca gctcttcttt
    36421 acgggtaccg gaacggttgt agtcgatagc cgggaagacg cgtttttcag cgatcttacg
    36481 agagaggtgc agttccatgt tgcctgtacc tttaaactct tcgtagataa cttcgtccat
    36541 tttagaacca gtatcgataa gcgccgtcgc gataatggtc aggctgccgc cctcttccac
    36601 gttacgcgcc gcaccaaaga agcgtttcgg acgatgaagg gcattggcgt ccacaccacc
    36661 ggtcaacact ttacctgacg ccggaacaac ggtgttgtaa gcgcgcgcca gacgagtgat
    36721 ggagtcgagc agaatgataa cgtctttctt gtgctcaacc aggcgtttcg ccttctcgat
    36781 caccatttcc gcaacctgaa cgtggcgaga tgcgggttcg tcaaaggtag aagcaacaac
    36841 ttcacctttt accagacgct gcatctcggt tacttcttcc ggacgttcgt cgatcagcag
    36901 aaccatcagc acacaatccg ggtggttgta agcaatgctc tgagcaatgt tctgcagcag
    36961 catggtttta ccggctttcg gcggtgccac aatcagacca cgctgaccac gaccgatagg
    37021 tgatgccaga tccagtacgc gagcagttaa atcttccgta gaaccgttac cacgttccat
    37081 acgcagacga gagtttgcgt gcagcggggt taagttctca aagaggattt tgttgcgggc
    37141 gttttcaggt ttgtcgaagt taacttcgtt aactttcagc agcgcaaaat agcgttcacc
    37201 ttctttcggc gggcgaatct taccagagat ggtatcacca gtgcggaggt tgaaacggcg
    37261 gatttggcta ggggaaacgt agatgtcatc aggaccggcg aggtaggagc tgtctgcgga
    37321 acggaggaaa ccaaatccat cctgcaatat ctccagtacg ccatcaccaa agatatcttc
    37381 gccactcttt gcgtgctgct tcaggatggc aaaaataatg tcctgcttac gcatacgagc
    37441 caggttttcc agccccatat tttcgccgag agtgatcagc tcagaaaccg gcgtattctt
    37501 taattcggta agattcatag tggtgtgagt tcttaaactt ggggtaaatc tcgaacttaa
    37561 tgttgtgaat ggtatggcag gatcatccat gcctgtttac ggtcatcgac tcatgtcttt
    37621 tcgctgcctg gtcacaggag agtacgcaga actgaaacga caagacggat tgagtgacga
    37681 gccagaaatc tgttcacttc gcatttaaga taaaacggga agcgttggaa attacaagat
    37741 tcaaacttaa taaggtatgt ttaatacgaa gtcaacacta agttagcatg actcacgccg
    37801 ggcgtccagt ttttagcgac ggggcacccg aacatgaaat tcccttacgc caggttagcg
    37861 tcgaggaact ctttcaactg acctttagac agtgcgccca ctttggttgc cgccacttca
    37921 ccgtttttga acagcagcag agtcgggata ccacggatgc catatttcgg cgcagtgcca
    37981 gggttttgat cgatgttcag ttttgcaacg gtcagtttgc cctgatattc gtcagcgatt
    38041 tcatccagaa tcggggcgat cattttgcac ggaccgcacc actctgccca gaaatcgacg
    38101 aggatcgccc cgtccgcttt gagtacatcc gtgtcaaaac tgtcgtcagt caggtgaata
    38161 attttatcgc tcatatataa ctccacagga ataagcctgg cgtgttggtt tcgttgttgg
    38221 tgtaacatta accaactaaa ggttgacttt atttcaccgg atacgctttc gtaaagcaat
    38281 agtaagctga tattctacca cactatgagc aaaacacatt taacagaaca gaagttttcc
    38341 gacttcgccc tgcatccgaa ggttgtagaa gcccttgaaa aaaaagggtt tcataactgt
    38401 acgcccattc aggcactggc ccttccgctg acgctggcgg gtcgtgacgt agccgggcag
    38461 gcgcaaaccg gtaccgggaa aacgatggcg tttcttacgt caacgtttca ttatcttctc
    38521 tctcatcctg cgattgccga tcgcaaggtg aatcagccgc gtgccttaat tatggcaccg
    38581 acgcgtgaac ttgccgtgca gatccatgcc gatgcggaac cgctggcgga agctaccggc
    38641 ctgaagctgg gtctggctta cggtggtgat ggttacgaca aacagctgaa agtgctggaa
    38701 agcggcgttg acattctgat tggcaccacg ggtcgtttaa ttgactacgc caagcagaac
    38761 cacattaacc tcggtgccat tcaggtagtg gtactggacg aagccgatcg catgtacgat
    38821 ctgggcttta ttaaagatat ccgctggctg ttccgccgta tgccgcctgc aaaccagcgc
    38881 ctcaacatgc tgttctccgc cacgctttcg taccgggtac gtgaactggc gttcgagcag
    38941 atgaacaatg ccgaatatat tgaagtggaa ccggaacaga aaacgggcca ccgtattaaa
    39001 gaagagcttt tctacccttc taacgaagaa aaaatgcgtt tgctgcaaac gctgatcgaa
    39061 gaagagtggc cagacagagc gattattttc gccaacacca aacaccgttg tgaagagatc
    39121 tggggccacc tggcggcaga tggtcatcgt gtcggtttgt tgacaggcga tgtcgcgcag
    39181 aaaaaacgtc tgcgtattct tgatgaattt acccgtggcg atctggatat tctggttgcc
    39241 accgacgttg ccgcgcgtgg tttgcatatt ccggcagtga cgcacgtctt taactacgat
    39301 ttacccgatg actgtgaaga ttacgttcac cgtattggtc gtacaggtcg cgcaggcgca
    39361 agcggtcact ctattagcct ggcgtgtgaa gagtatgcat tgaatttgcc tgctattgag
    39421 acctatattg gtcactcaat tccggtaagc aaatacaatc cggacgcatt gatgaccgat
    39481 ctgccaaaac cgctgcgcct cacgcgcccg cgtacaggca atggtccgcg tcgtactggc
    39541 gctccgcgta atcgtcgtcg ttcaggttaa ataaaatgtc atactcaaaa tcattcaaac
    39601 tgtatcaaga tggcaagaga atgaatcctg aatcacttac tgtagtaagt gattcaggtg
    39661 aaagaatgca gccaacacag agacagattg aaggatgaag agtatgggtt ccacctcgtc
    39721 gctgtatgca gccattgatc tcggttcgaa tagttttcat atgctggttg tgcgcgaggt
    39781 ggctggaagc atccagacgc tgacgcgaat aaaacgcaaa gtgcgtctgg ctgctggcct
    39841 gaatagtgaa aatgccctct ctaatgaagc aatggagcgc ggttggcaat gtctgcgcct
    39901 gtttgctgaa cgtctgcaag atattcctcc ctcgcaaatt cgcgttgtcg ctacggcgac
    39961 gttacgcctt gccgtcaatg cgggtgattt tattgccaaa gcgcaggaaa ttctcggttg
    40021 tccggtacag gtgatcagcg gtgaagagga agcacgtctg atttatcagg gcgttgctca
    40081 caccactggt ggtgccgatc agcgcctggt ggtggatata ggcggagcca gtactgaact
    40141 ggtaaccggc acgggtgcac aaactacctc gttgttcagc ctgtcgatgg gctgcgtcac
    40201 ctggctggaa cgctattttg ccgatcgtaa tctggggcag gaaaattttg atgctgcaga
    40261 aaaagcggca cgcgaagtgt tacgtccggt tgccgatgaa ttacggtatc acggctggaa
    40321 agtgtgcgtt ggtgcttccg gcaccgtgca ggcgttacag gaaatcatga tggcacaggg
    40381 gatggatgaa cgcattaccc tggaaaagtt gcagcaattg aaacagcgag ccattcattg
    40441 cggtcggctg gaggaactgg agattgacgg gctgacgctg gaacgtgcgt tagtgttccc
    40501 gagtggtctg gcgatcctga tcgccatttt taccgaactg aatattcagt gtatgaccct
    40561 ggcgggcggt gcgctgcgtg aaggcctggt ctacggcatg ttgcatctgg ccgtcgagca
    40621 ggatattcgc agccgtacgc tgcgtaatat tcaacgccgc tttatgatcg atattgatca
    40681 ggcacagcgc gtagccaaag ttgcggctaa cttcttcgat caggtggaaa acgaatggca
    40741 tcttgaagca ataagccgcg atttgctcat cagcgcctgt cagcttcatg aaatcggcct
    40801 gagcgttgac ttcaaacaag cgccgcaaca cgctgcttat ctggtgcgta atctggatct
    40861 tcccggtttt acccccgcac agaaaaaatt gctggcgacg ctactgctca accagactaa
    40921 tccggtcgat ctctcatcgc tgcatcagca aaatgccgta ccgccgcgcg tcgcagaaca
    40981 actctgccgt ttactacgcc tggccatcat ttttgccagc cgtcgccgtg acgatctcgt
    41041 gccagagatg acattacagg ctaaccatga actgttgacc ttgacgcttc cgcaaggttg
    41101 gctaacccaa catccgctgg gtaaagagat tattgctcag gaaagccagt ggcagagcta
    41161 tgtccactgg ccgctggaag tgcattaatg agtaagtgcc ggatgcgatg ctgacgcatc
    41221 ttttccggcc ttgattattt ccctcgtttt gccgccatca tcgctttcag attcgccaga
    41281 tggctttgcc ctttctgcat ccgttcttcg gcgctgacca ctttgcgctc ctgttcccaa
    41341 atcagatcat cctgcggcag ctccagcaaa aagcggctcg gctccgggcg caccagttcg
    41401 ccgtactgac ggcgttcttt acacagcgta aaggtcaatt ccttctgggc gcgggtaatg
    41461 ccgacatagg ccagccgccg ctcctcatcg atattatctt catcgatgct gctctggtgc
    41521 ggcaaaaacc cttcttccat accgaccatg tagacataag gaaactccag ccctttcgac
    41581 gcgtggagag tcatcagttg cacctgatcc agctcttctt cactctcacc acgctccatc
    41641 atgtcgcgca aagtaaagcg cgtcaccacc tgggttaacg tcatcggctc atccagttca
    41701 ctgccttcga gcatctccgt catccagcta aacagttggt tgacgttctt catgcgcatt
    41761 tcggcggctt tcgggctggg cgatgtttcg tacagccagg attcataatc catgccgtgg
    41821 atcagatcac gcaccgcggc aatcggctcc cgctccgcca gacgctggat ttctgccaac
    41881 cagtgagtga agcgggtcaa tgcttcataa ccacgtccgc taagcgtctg actcaggccc
    41941 atatcaaagc tggcggtaaa catgctttta ttgcgcgtca tcgcccattc acccaacttt
    42001 tttagcgtcg ccgggccaat ctctcgcttc ggcgtgttaa cgatacgcag aaatgcgctg
    42061 tcatcgtccg ggttagtcag cacgcgcaga taagccagca agtccttgat ttcaggacga
    42121 gagaaaaacg acgtaccacc agaaattttg tacgggatgc ggttttgcat caggaatttt
    42181 tcaaacaccc gcgactgatg gttaccgcga taaagaatgg cgtaatcttt gtactgcgtt
    42241 ttattgacga agtgatgggc gatcagctcg ccagtaacgc gctcggcttc atgttcctcg
    42301 ttattcgcgc ttaaaacttt tagctctgtg ccgtaaccca gttcggagaa cagacgcttt
    42361 tcaaagacgt gcggattatt ggcgatcagg atgttcgccg ctttcaaaat acgtccggaa
    42421 gagcggtagt tctgctcaag cttaatcacc ttcagcgccg gaaaatcctg actcagcagc
    42481 accaggtttt gtggacgtgc gccgcgccag gagtagatcg actggtcgtc atcgcccacc
    42541 acggtaaagc gcgcgcggct gcccaccagc agtttcacca gctcatactg gctggtgttg
    42601 gtgtcctgat actcatccac cagcagatag cgaattttgt tctgccagcg ctcgcggact
    42661 tcttcattgc gttgcagcag caacgtcggc aataaaatca gatcatcgaa gtcgagaacg
    42721 ttacaggctt tcaggtgtgc atcatacagc ccataacaat gggcaaaaat acgatcccgc
    42781 tcgccctttg cctctgccgc cgcctgcgcc ggtgttttga gatcattctt ccagttagag
    42841 atggtcgaaa tcagttgttg caggagaact ttgtcatctt caatcagccc ctcggtcaac
    42901 tctttaagca aagcaagctg atcggtatcg tcaaacaacg agaagttcgc tttcatccca
    42961 agcgccgcat actcgcgttt gatgatatcc agccccaacg tatggaaagt ggagatcatc
    43021 agcccacgcg cctctttgcg ccccagcgtc tgccctacac gctctttcat ctcgcgcgct
    43081 gctttattag taaaggtcac cgccgcaatg tgccgcgcct gataaccgca accgcggatc
    43141 agatgggcga ttttattggt gataacacga gttttaccgg aacccgcgcc cgccagcacc
    43201 aggcaggggc cggtaacgaa ttcgacagct tgttgttggc cggggtttag acgcataggt
    43261 gtattgctca atcttcgaac ggggggagga ttgtagcaga atcgcatttg aatgtgaccg
    43321 cgtgtaagcc gctaaccggt tggctgcgga tgtttaaatc agagagagaa gaccgaaatc
    43381 ggagttcatc gtgaatccat tagacttaga aaatatcggg tcgtaacgtt gtgttattac
    43441 tttttcagag tttactaagg agaggtttac tcgtggcgat taatttcagc cctaaggttg
    43501 gtgaaatact ggaatgcaac tttggtaact atcctgttag ccaaaatgga ccgttcatca
    43561 ccacgtatta cgatggccgc attcctcctg aaatgataaa aaacaggtta gttgtcgtgc
    43621 tcaacggaaa aattaatggc aacgcatgta tcgtcgttcc attatcaacc actcgcgacc
    43681 atgacaagtt aaatcgtgga atgcatgttg aaatagccag taatattatc aatgacctgc
    43741 aattctttga tcagcagatt cgttgggcca aagccgacct ggtacaacaa gtcagtcgaa
    43801 acagattaaa cagagcaaga acttacagag ggtatcttaa tcaatgtttg ccacatgagt
    43861 tggttgcaga catacagagg gcggtcatca agtcaatcaa tgccatatcg ctcataaatt
    43921 gacaataaaa tcaccatccc cgataatgac tctgtagccc atagaggccc taagttttct
    43981 ccccttgagg gacctgcgaa gcccggattt tccgggcttt tgccatatat gtgacccgtc
    44041 ctgactgaca attacgttag catctgcgga tcatgctaaa atcgccgcct gacaattatt
    44101 cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg taaaagaaga
    44161 gaaactggct ctggatcttc tcgagcagat taagaacggg gccgattttg gcaagctggc
    44221 gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg aattccgcca
    44281 gggtcagatg gttccggcgt tcgataaagt tgtgttctct tgtccggtac tggagccgac
    44341 tggcccgctg catacccagt tcggttacca catcattaag gtgctttacc gtaactaaca
    44401 gtaaggcctt ctccaggaga aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa
    44461 tgcgtttcat atctgtcata tagccgcgca gtttcttacc tacctgctca atcgcatggc
    44521 tgcgaatcgc ttcgttcaca tcacgcagtt gcgcgttatc taccgcgcct tccggaatag
    44581 ctttacccag gtcgcccggt tgcagctctg ccataaacgg tttcagcaac ggcacacaag
    44641 cgtaagagaa cagatagtta ccgtactcag cggtatcaga gataaccacg ttcatttcgt
    44701 acagacgctt acgggcgatg gtgttggcaa tcagcggcag ctcgtgcagt gattcataat
    44761 atgcagactc ttcaatgatg ccggaatcga ccatggtttc gaacgccagt tcaacgcccg
    44821 ctttcaccat cgcgatcatc agtacgcctt tatcgaagta ttcctgctcg ccgattttgc
    44881 cttcatactg cggcgcggtt tcaaacgcgg ttttgccggt ttcttcacgc caggtcagca
    44941 gtttcttatc atcgttggcc cagtccgcca tcataccgga agagaattcg ccggagatga
    45001 tgtcatccat atgtttctgg aacagcggtg ccatgatctc tttcagctgt tcagacagcg
    45061 cgtaagcacg cagtttcgcc gggttagaga gacggtccat catcagggtg atgccgccct
    45121 gtttcagcgc ttcggtgatg gtttcccagc cgaactgaat cagtttttct gcgtatgccg
    45181 ggtcggtgcc ttcttccacc agcttgtcga agcacagcag cgagccagcc tgcaacatac
    45241 cgcacagaat ggtttgctcg cccatcaggt cagatttcac ttccgcaacg aaggacgatt
    45301 ccagcacgcc cgcacggtga ccgccggtcg ctgccgccca ggctttggca atcgccatgc
    45361 cttcaccttt tggatcgttt tccgggtgaa cggcaatcag cgtcggtaca ccgaatccac
    45421 gtttgtactc ttcacgtact tcggtgccag ggcatttcgg cgcaaccatt actacggtga
    45481 tgtctttacg gatctgctcg cccacttcta cgatgttgaa accatgcgag taacccagcg
    45541 ccgcgccgtc tttcatcagt ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg
    45601 gcgtcaagtt aaccaccaga tccgcctgcg ggatcagttc ttcgtaagta ccaactttaa
    45661 agccgttttc ggtcgcttta cgccaggacg cgcgcttctc ggcaatcgct tccttacgca
    45721 gcgcgtagga gatatccaga ccagaatcac gcatgttcag gccctggttg agaccctgtg
    45781 cgccacagcc gacgatgacc acttttttac cctgaaggta gctcgcgccg tcagcgaatt
    45841 cgtcgcgacc cataaagcga catttgccca gctgtgccag ctgctggcgc aggttcagtg
    45901 tattgaagta gttagccatg gtgattcctc gtgatgttgt gcttcttatt gttcggttcg
    45961 cttttgcgag attgaattca ctatatgaca ggaaatttat tgcggaaatt gatatattca
    46021 caacgtcaca ttgcaatttt tgcaacgtca acatcgaggg ctgtccctgt ggatttacgc
    46081 gatctgaaaa ccttcctgca tctggcggaa agccgccatt ttggccgcag cgcgcgggcg
    46141 atgcacgtta gcccatccac gctctcacgg cagattcagc gcctggaaga agatctcggt
    46201 cagccgctgt ttgtgcgcga taaccgcacg gtgacgctga ctgaagcggg cgaagaactg
    46261 cgcgttttcg cccagcaaac gctgttgcaa tatcagcaat tgcgccacac catcgaccag
    46321 caaggaccgt cgctctctgg cgaattacat atcttctgct cggtgaccgc tgcctacagc
    46381 catctgccgc cgattctgga tcgcttccgc gcggaacatc cgtcggtgga gatcaaactc
    46441 accactggtg atgcggcaga tgcaatggaa aaagtggtca cgggagaagc ggatctggcg
    46501 attgccggga aaccggagac gctacccggc gctgtggcgt tttcgatgct ggagaatctg
    46561 gcggtagtgc tgattgcgcc cgcgctgccc tgcccggtgc gtaatcaggt ctcggtagag
    46621 aagccggact ggtcaacggt accgtttatc atggccgatc aggggccagt acgccgccgc
    46681 attgaactgt ggtttcgacg caataaaatc agcaacccaa tgatttacgc cacggttggc
    46741 gggcatgaag cgatggtatc gatggtggcg ctcggctgtg gtgtggcgct gttgccggaa
    46801 gtggtgctgg aaaacagccc ggagcctgta cgcaaccggg taatgatttt agatcgcagc
    46861 gatgagaaaa cgccgtttga actgggcgtg tgtgcacaaa aaaagcggct gcatgagccg
    46921 ctgattgagg cgttctggag gattttgccg aaccacaaat gacgttgtcg cgcgagtagg
    46981 cctgataagc gaagcgctat caggcatttt tccctaaccc gccaaaaaga acctgaacgc
    47041 cgggttattg gtttcgtcgt ggcaatcgta gcccagctca tttagccggg tttcgaaatc
    47101 cggttcatgg tcgccaagct cgaacgccgc cagtacacgc ccgtagtcgg taccgtgact
    47161 gcgataatgg aacagagaaa tattccagtg cgtacccagc gtgttgagga agcgcagcag
    47221 cgcacccggc gattccggaa attcgaagct gtagagacgt tcctgcaacg gatgcgatgg
    47281 acgcccccca accatatagc gcacgtgtaa cttcgccatt tcgtcgtcgg agagatcaac
    47341 cacgctgtag ccgccgtcgt tgagcatctg caaaatttct ttgcgctctt cgaggccgcg
    47401 gctcaggcgc acgccgacaa agatgcaggc gtttttggca tcggcaaaac ggtagttgaa
    47461 ctcggtgacc gatcgcccgc caagcagttg gcagaatttg aggaagctgc ctttttcttc
    47521 cggaatggtc accgccaaca acgcttcacg ctgttcgccc agttcgcagc gttccgagac
    47581 gtagcgcagg ccgtggaagt tcacgttggc accggaaaga atatgcgcca gccgttcgcc
    47641 gcgaatgttg tgctgggcga tatatttttt cattcccgcc agcgccagcg cgccagaggg
    47701 ttccgccacc gcgcgcacat cttcgaataa atccttcatc gccgcacaga tcgcatcact
    47761 atcgacggtg atgatatcgt cgagatactc ctggcacaaa cggaaggttt cgtcgccgat
    47821 gcgttttacc gcaacgcctt cagcaaacag ccccacgcgc ggcagatcaa ccggatgacc
    47881 cgcatccagc gctgctttca ggcaggcgga gtcttccgct tctacggcga tcactttgat
    47941 ttgcggcatc agttgtttga tcagcaccgc cacgcccgca gccagaccgc cgccgccgac
    48001 tggcacaaat acgcggtcga gatgggcgtc ctgctggagc agttccagcg ccagcgtgcc
    48061 ttgcccggca atcaccatcg gatgatcgaa cggcggcacc caggtgaacc cctgctgctg
    48121 tgacagttcg atcgctttgg ctttcgcttc atcaaagttc gcgccgtgga gcagcacttc
    48181 gccgccgaag ccgcgcaccg cgtcgacttt gatatcggcg gtggcggttg gcatgacgat
    48241 cagcgccttc acgcctaacc gtgcggaaga aaacgcgacg ccctgcgcgt ggttacccgc
    48301 agaagcagtg atcacgccgt gcgctttttg ttcttccgtc aggcccgcca tcatggcgta
    48361 tgcgccgcgc agcttaaagc tgtgcactgg ctggcgatct tcgcgcttca ccagaatgac
    48421 gttatcaaga cgcgacgaca gtttttccat tttttgtagc ggcgtaacct gcgccgcctc
    48481 gtaaaccggc gcgcgcagca ctgctcttaa atattcggca ccttccggag caccggacag
    48541 gggttgcgag tcagccatta ttaacccccc agtttcgatt tatcgcgcac cgcgcctttg
    48601 tcggcgctgg ttgccaggct ggcataagca cgcagggcaa aggagacttg acgttcacga
    48661 tttttcggcg tccaggcttt gtcaccccga gcttcctgcg cttcacgacg cgccgccagt
    48721 tcggcatcgc ttacctgtaa ctgaatgcca cggttcggaa tgtcgatggc gatcaggtcg
    48781 ccgtcttcaa tcaggccaat gctgccgccg cttgccgctt ccggtgagac gtggccgata
    48841 gaaaggccag aggtgccgcc agagaaacga ccgtcggtga tcagcgcaca ggctttgccg
    48901 aggcccattg atttcaggaa gctggttggg tagagcattt cctgcatgcc agggccgcct
    48961 ttcggacctt catagcggat caccaccaca tcgcccgcga caaccttgcc gccgagaatc
    49021 gcttctaccg cgtcgtcctg gctttcgtat actttcgccg ggccggtgaa tttgaggatg
    49081 ctgtcatcga cgcccgcggt tttaacgatg cagccgtttt ctgcgaaatt accgtagagc
    49141 actgccaggc cgccgtcttt gctgtacgca tgctccagcg agcgaataca gccatttgcg
    49201 cgatcgtcgt ccagcgtatc ccaacggcaa tcctgcgaga atgcctgtgt agtacgaatg
    49261 cccgccgggc ctgcacggaa catatttttt accgcgtcat cctgggtcag cataacgtcg
    49321 tattgttcca gcgtttgcgg caacgtcagg ccaagtacgt ttttcacatc acggttcagt
    49381 aaccccgcac gatccagctc gccgagaata ccgataacac caccagcacg gtgaacatct
    49441 tccatatggt atttttgggt gctcggcgca actttgcata actgcgggac tttacgcgaa
    49501 agtttgtcga tatcactcat ggtgaagtcg atttccgctt cctgcgccgc cgccagcagg
    49561 tgaagtacgg tgttagtcga tccacccatc gcgatatcca gcgtcatggc attttcaaac
    49621 gccgctttgc tagcaatatt gcgaggcagt gcactttcgt cgttttgctc gtagtaacgt
    49681 ttggtcaatt caacaatgcg tttaccagca ttgaggaaca gctgcttacg gtcggcgtga
    49741 gttgccagca gcgagccgtt gcccggctgc gacaggccca gcgcttcggt caggcagttc
    49801 attgagttag cggtaaacat cccggagcag gaaccgcagg ttgggcatgc ggaacgttca
    49861 acctgatcgc tctgggagtc agaaactttc gggtctgccc cctggatcat cgcatcaacc
    49921 agatcgagct tgatgatctg atcggacagt ttggttttcc cggcctccat cgggccgccg
    49981 gaaacaaaga tcaccggaat attcaggcgc agagaggcca tcagcatccc cggggtgatt
    50041 ttgtcacagt tagagatgca gaccatggcg tcggcgcagt gggcgttgac catatactca
    50101 acggaatcag cgatcagttc gcgagatggc agtgaataaa gcatcccccc atggcccatg
    50161 gcaatcccat catccaccgc aatggtgttg aactctttgg caacgccgcc agccgcttca
    50221 atttgttcgg cgaccagttt accgagatcg cgcagatgga cgtgacccgg tacaaattgg
    50281 gtgaacgagt tcacaaccgc gataatcggc ttaccgaaat cggcgtcggt cattccggtg
    50341 gcgcgccaca gcgcacgagc acccgccata ttacgaccat gagtggtggt ggcggaacgg
    50401 tacttaggca tactttattt actcccagtg tctgtctcgt aaatgggacg gtgcgtgccg
    50461 tcccattttt tgtatttatt gattaacttg atctaaccag ccccatttat cttcggtttc
    50521 gccggtgaag aggccgaaga aggcttgctg aatacgtttg gtaaccgggc cacaacggcc
    50581 ttcgccaacc tgaataccgt ctacgctgcg cactggcgtg atttctgccg ccgtaccgga
    50641 cataaacact tcatccgcca ggtacaggga ttcgcgcgac agtacctgct cacgcacttc
    50701 aattcccagc tctttcgcca gtttgatgat ggcatcacgg gtaatacccg gcagcgcgga
    50761 ggaggtgaac ggtggggtga acagcacacc atctttcact tcaaacaggt tttcgcctgc
    50821 gccttcagag atgtaaccat tcacatccag cgcgatacct tcctgataac cgtggcggcg
    50881 tgcttcgcta cccaccagca gggaagagag atagttacca ccggctttcg ccgcggttgg
    50941 gatggtgttt ggtgctgcac ggttccagga ggaaaccatc gcatcgatcc cctgctccag
    51001 cgcttctgcg cccagatacg ctccccacgg gaaagcggcg ataatcacat cggttgagta
    51061 tcccgctggc gggttaacgc ccataccaac atcaccgacg aagatcagcg gacggatata
    51121 ggcgctggtg agattgtttt tgcggatcac gtcacgacaa gcttccatca gctcatcaat
    51181 gctctgcgaa accgggaagc gatagatttt ggcggagtca tgcagacgct gcatatgctc
    51241 acgatggcgg aatacaaccg gtcctttgtg cgagtcgtag caacggatgc cttcaaaaac
    51301 cgaggtgcca tagtgcagcg cgtgcgacat cacatgcacc ttcgcgtctt cccagcgaac
    51361 catctcccca ttgaaccaaa tgtaatcagc tttcttcgtg gtcattttta tattcctttt
    51421 gcgctcaggc gcggatttgt tgtgatgtgg ttgtgctctg gcagatggca acgtgtgcga
    51481 cgtccaccag tttatttaac tgactaaaca gtaagtcgac cgaccgtggg ctggcaacgg
    51541 tcaattcgat atttatattt tgtgcatcgc tggcggcggc catattcatt gagcagacgt
    51601 ggaaaccacg atgacgcacc acgcgtaaaa cacgttctaa ggtttctgga ttgaagcgag
    51661 ccgatacatt gacctgatgt tgcatcatga taatttctcc aacatttctg aattactggc
    51721 gccaggcggc accagcggcc agacgttctc aagttcgtcg attgagacat gaagcaggta
    51781 tggcccatca ctgttcagca tggtgtcgag tgccgcttca acctggtctt tacgggtgat
    51841 gtgttggcca gggatgccga aggcgctggc taacatgagg aaatcggggt tatcagtaag
    51901 ggtggtttcg ctgtatcgtt cctgaaaaaa cagttgctgc cattgtcgaa ccatccctaa
    51961 ccgttggtta tcgagtaaga cgattttcaa cggtaactgc ttgcgtttta cggtgcccag
    52021 ctcttgcaca ttcatcatga aagagccgtc accggagata cagacaacgg tatcgttcgg
    52081 tcgcgcgact tgtgcgccaa ccgccgccgg taaaccaaaa cccatggtgc ctaagccgct
    52141 ggaggtaatg aaattttccg ggcgagtgtg tgcgatgtgc tgcgcggccc acatctgatg
    52201 ctgccccaca tctgtggtca caacgcaatc cgcaggttta cgatccgaca gttgttttaa
    52261 caacaacggc gcgtagatag tgtcaccggg atggtcgtaa cgccaggcat gttcatcact
    52321 cagctgcgcg cagtattgct gccagtcatt gatatttaat ggctgctgta atgctggtaa
    52381 cagcgcattt aaatcaccct gtaatgccac atgtgcctga cgcagcttgt tcatttctgc
    52441 cgggtcgata tccatatgga taacactggc gtgtggtgcg aaggtgttca gtttgccggt
    52501 cacccggtca tcaaaacgtg cacccacggc gatcagcaag tcgcactcct gcaccgcgaa
    52561 gttcgccgct ttggtgccgt gcattcccag cattcccaga tagtacgaat aatctgctgc
    52621 aactgcgccc agccctttca gcgtgcaggt ggcaggcatt tttgtggtag agagaaattc
    52681 tcgtaaagca ggaactgcct gcgccatacc cacgccacca ccaacgtaca gcatcggttt
    52741 ttgcgctttt gccagcatct ggcgcgcttg ctcgacttcg gcatggggga aagtcacttc
    52801 gttttcaacg gtggtgaacc acggttccag gtcgccgctg gctaactgga tatcttttgg
    52861 gatatcgacc agaaccggac caggacgacc tgagctggca acgtcgaatg cttcagccat
    52921 gatgcgcggc aactcttcca gcgactgcac caggaagctg tgcttagtac aggctaacga
    52981 caatcccagg acatccactt cctgaaatgc gtcagtgccg ataaacggtg cggacacttg
    53041 accggtgatg gcaacaacag ggatagaatc taacagtgcg tccgcaagcc cggttatcag
    53101 gttggttgct cccggaccag acgtggcgat acatacgcca gttttgccgg tagcacgagc
    53161 ataaccgata gccgccattg ccgcgccctg ttcatgtcgg cacagcaagt gctccacgcc
    53221 gccgtcatac aatgcatcgt aaaccggcat aattgcgcca cccggataac cgaaaacggt
    53281 gttcacaccc tgtgcccgca acgcatgtac cacccactgt gcgccattca tagttagttc
    53341 cccgtcctga atcttgagaa acagaatttt gtgctgttat tcattgtctg ctcctcggtt
    53401 atgtttttaa ggtcaaaaaa aacccccgga cctttcggtg cgggggtctt agttcgttaa
    53461 ggcttgatct ctaagccttt cctcgtccaa gtgcagcccc gcacggtggg ataataatca
    53521 ccaccacgct aatcacgacc aggctaatca ctcgtagaag ggctgtcatt ttgtcttttc
    53581 ttgcatcttg ttcgaaggaa tgcctaaaga gttaccatag attttgcgaa tagcacaaga
    53641 tattttttgg ccgattttta tgatgccttt aataatttca atagaaattg ttgtttttta
    53701 aaaagaaaat agtgagtgaa aaaaattcaa tttcagtata aatgttgttc ccttcctcgt
    53761 agttcatcct ttgtccgctg catttttgcg agcatcattc caccgccaga acaactcaac
    53821 ttcacttgca caaataaaca ggaatcagcc actaaaattc acgaattagc gggctacagc
    53881 agcgatatct tatgcacata atggtgactc aaggagggct tatgtcactg tcaattgttc
    53941 atacccgcgc agccctggga gtaaatgcgc cccaatcact gttgaggtac atatcagtaa
    54001 aggtctaccc ggcttaacga tggtgggctt accagaaaca acggtaaaag aagcccgcga
    54061 tcgcgtgcgc agcgccatta tcaatagcgg atatgaatat ccggccaaaa aaatcaccat
    54121 caacctagct ccagccgatc tgccaaaaga agggggacga tatgatttac ctatcgccat
    54181 tgcgttgctg gcggcctcag aacagcttac agccaataag ttagatgaat atgaattagt
    54241 cggagaactg gcgcttacag gcgctctgcg tggcgttccc ggtgcaatct ccagtgcaac
    54301 tgaagctatt aagtcgggca gaaaaattat cgtcgcgaaa gataacgaag atgaagtggg
    54361 gctaattaac ggtgaaggat gcctgatagc caatcatctg caggctgtct gtgcgtttct
    54421 ggaaggtaag cacactctcg aacgcccgaa accaactgat gcagtatccc gggcgctaca
    54481 acatgatctc agtgatgttg tcggtcagga gcaaggaaag cgaggactgg aaattaccgc
    54541 cgctggcggg cacaaccttt tactgatcgg accgccggga acaggtaaaa caatgctcgc
    54601 cagccgtatt aatggtcttt tgccagattt aagcaatgaa gaggcactgg agagcgctgc
    54661 gatattaagt ctggtaaatg ctgaatcagt acaaaaacaa tggcggcagc gcccgttccg
    54721 ctcacctcat cacagtgcgt cgttaactgc gatggtgggc ggtggtgcaa ttccagggcc
    54781 tggtgaaatt tcgttggcac ataacggcgt gctttttctt gatgagctac ctgaatttga
    54841 acggcgtaca ctggatgctt tgcgagagcc gattgaatcc gggcagatcc atctttcacg
    54901 cacacgagca aaaataacct atccagcccg tttccagctt gttgcggcga tgaatcccag
    54961 ccctaccgga cattatcagg gaaaccataa ccgctgcacg ccagaacaga cattacgtta
    55021 tctcaaccgg ctctcggggc cctttctcga ccgcttcgat ctctcactgg agatcccatt
    55081 accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagca ccaccgttaa
    55141 acaacgtgta atggctgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg
    55201 gctggatagt ccggaaatac gcaaattctg caagcttgag agcgaagatg cgcagtggct
    55261 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tattgaaagt
    55321 tgcacgaacc attgctgata ttgatcagtc taacattatc acacgtcagc atttgcagga
    55381 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
    55441 aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct tcagcacctt
    55501 caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta atacgcgtca
    55561 tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca cggcatactg
    55621 ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga
    55681 aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca ccatgacgag
    55741 agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg
    55801 ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg
    55861 cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca
    55921 ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc
    55981 gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc agacaactgg
    56041 aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt ttaaccgctg
    56101 ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgta
    56161 aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc gcctcattgt
    56221 gggaatgtat gcttaatcag tggctgggac gtttgtatca aaatcaggat gcccatacag
    56281 gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ctgcatgaac
    56341 gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt agtagtcagt
    56401 tgctggggta tttcacttta gcgctttata ccagtgcccc ttccaaacta aagggagatc
    56461 ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca ggtttgatcg
    56521 gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag caacagattg
    56581 cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa ggcggcctac
    56641 acaccgttgt cgatagcaca acactttcgc ggccgcttta tgccatatgg ctgcaaaata
    56701 gcgataaaaa tacgctgatt cgcgatctat tgaaaattaa cgtgctggat gaagtgtatt
    56761 aatctgaatg gctggcaagg atgccggtag aaggatttac ttcagagagg gttatttcag
    56821 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
    56881 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
    56941 gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac
    57001 cgactgaact accgctccac cgaattcttt tacaaccacc ggttttatga ccggcttact
    57061 gcttaatttg atgcctggca gttccctact ctcgcatggg gagaccccac actaccatcg
    57121 gcgctacggc gtttcacttc tgagttcggc atggggtcag gtgggaccac cgcgcta
//
LOCUS       DAZFKF010000024        53614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFKF010000024 DAZFKF010000000
VERSION     DAZFKF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.024"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(170..742)
                     /locus_tag="ACHKA1_001764"
     CDS             complement(170..742)
                     /locus_tag="ACHKA1_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679428.1"
                     /translation="MLTKKMLLVCASCVALSACSAQEIAELNRQISDGAFAVTKVLNV
                     GVNQADDDLKYLSQAPRAKREKRDHVIEVPVDVDTAAGRLKRYYKFYSNEEVDAIRNN
                     GHANGKWAAAAISQNGHMWDAMPGSYYKMGSDWDGGEFDDHLTLELEKNGKGSRIYIT
                     YASPAPGHLKDKFIEPLVKRVKDVAEGRVR"
     gene            934..1109
                     /locus_tag="ACHKA1_001765"
                     /pseudo
     CDS             934..1109
                     /locus_tag="ACHKA1_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976766.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1155..1418)
                     /locus_tag="ACHKA1_001766"
     CDS             complement(1155..1418)
                     /locus_tag="ACHKA1_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO5679429.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVGTQKGE"
     gene            1608..1847
                     /locus_tag="ACHKA1_001767"
     CDS             1608..1847
                     /locus_tag="ACHKA1_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679430.1"
                     /translation="MNNESEKAMWRCKPVDNPWTFTSLLKKAVCAVLPDFTPAQDKPQ
                     NMFSLSNEKDTDYSVPMRPFALYDPWKHHHDNADF"
     gene            complement(1898..3394)
                     /locus_tag="ACHKA1_001768"
     CDS             complement(1898..3394)
                     /locus_tag="ACHKA1_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO5679431.1"
                     /translation="MTTNNYLEYFLTLLGWVVNNGLWNVLISTGLFALPLAFRIIGIW
                     LKVREEGADEGNKGMLSLPRIEHVLYGAFFVMISCCVPLVQVTLDTLSFDKSRAQTCG
                     VWTPEEPGETGYSGVISSIGDQTAAAPVWWVVVHKLSKGVTQAAVASIPCRPDLRQLR
                     FEIQRTFIENRALADELQDFTNDCYSLALYMWKQRDQGQSKDKKVLDDISWIGSATFM
                     QGDYGTLQSKMPRAAFPWNTARDSGRPDTGRGGYPTCREWWSDSSVGLKKRVMEEVSD
                     HYWLRLSAAMKMIGSSQVDYKEEVIKRLVSPANLTLSQGGEVYRGYGGNADMTTMNAV
                     ARIAGTAGSAVAALGAFPALDAMRQALPMVQSIILMAIYIMIPLILAFAAYELSTLIT
                     LTFVVFAVHFLTFWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVMGT
                     MFLVLPAVWLGALSWAGVSVGTVMGDAINKGTSNVSKAAAKSGDLANK"
     gene            complement(3401..3742)
                     /locus_tag="ACHKA1_001769"
     CDS             complement(3401..3742)
                     /locus_tag="ACHKA1_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679432.1"
                     /translation="MSRIYGRVKKRMKVFALYTLLLTGITVFSLWLADTGIRHAEGFA
                     ALREWMYETRYGWLGWRLCLYSAIGWLGWRIWHAPGFRAEYRASFLRMATVSVLFILL
                     CEYAIFAEPGR"
     gene            complement(3747..5168)
                     /locus_tag="ACHKA1_001770"
     CDS             complement(3747..5168)
                     /locus_tag="ACHKA1_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO5679433.1"
                     /translation="MKQKMTIPVLAVVLCAGTVQADGNFLSLPKVNDSAFGYGKSVDG
                     AVSDKLFYTLGGGSVISQPATRSNMQKLGVDLGWSSDLMCGNFDLKTTVGNQLNGITD
                     GFKNLMGDVIQGASGAVASLPAMVIQRANPGLYDMLTNGVLQANVAFDKAQLNCQNMA
                     KRMMEFTDSNKWTQAAMLEEYKKIVNTGDGDAIKGDEAGRQVTGEEGQRWIGGQQRGG
                     AGQSAIRPTHDLTAAGFNMMNSLPSTSTSSVSPGNCNGSACMKYRNAEEAARAVVKVL
                     GDKSMRTCGDAKACTSGDADQQPGTSIAGTGFAPMLEEATRVNTELLAGMVNGRDKPT
                     AGNLAKLKAGSLSVTAGVIRALQRDPDNGALTSRLAGELAMADTVETALLMRRMLLTG
                     MAEPYAAAQPAALEEGERRIEALDREIMALKNEMELKRDLARNSVLTIIERDNERVGN
                     NPMIQQADNADSRIHSLDAPDKE"
     gene            complement(5182..6156)
                     /locus_tag="ACHKA1_001771"
     CDS             complement(5182..6156)
                     /locus_tag="ACHKA1_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFO5679434.1"
                     /translation="MISCTSRHRAGVLLLSMAVLPVAEASLNTAQIVASAASQSCISW
                     RVSGICYWLFCTKFSCKVRTSVKVSHYIPEVVVSTYTAPGGNPWREMSAVSSTAGGPE
                     NAISKGIAGLSAGGGNPADMKVQGERRSSIRFKYADATGHPATTLIGGMVPGYSCESP
                     VTPFVPYFLSTLDSIAWRTGLPESLYPEALIPGKREIGSQEEENMWGNVYPRSGFVTQ
                     TDDDKAAAVVAQRVADIITRTGEPHVYQPVKAGKKDGYWPPDPVEENTGTRNHKWQRL
                     TPSVSTSCAVFPDGEHEAAETGNAAFALWQPYSCCKKRGQTFLGSTNF"
     gene            complement(6153..6548)
                     /locus_tag="ACHKA1_001772"
     CDS             complement(6153..6548)
                     /locus_tag="ACHKA1_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFO5679435.1"
                     /translation="MYRLLLYSFLILPVAASAGTTIYTDSHQRPMNPPAGVRVVLLDA
                     PEQTQDTFFGVLPAEPAEASARVMERMQSPEWQSFQAGLAEHYRALAHAWSLGLKKYP
                     AVVFDDSEVVYGTTDVVLAEQLRTGGGQP"
     gene            complement(6684..7316)
                     /locus_tag="ACHKA1_001773"
     CDS             complement(6684..7316)
                     /locus_tag="ACHKA1_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5679436.1"
                     /translation="MLLIAGHLRPAFQAHTGRFLIADLSEEVVRRYLRFCTPLSGMAV
                     SPSSLLLQIRPQMSVLAECLIRQLITDEPSSVYFRDAVAFSSLALFEEHPDFHALLTG
                     CIRSCTVRVKNIISADLSRPWMLGEVASLLYISESLLKKKLQEELTTFSELLLKERMQ
                     YAAQLLSAGENRISRVAELCGYASASYFISVFRRYYGITPNQWLPVMQPD"
     gene            complement(7508..7786)
                     /locus_tag="ACHKA1_001774"
     CDS             complement(7508..7786)
                     /locus_tag="ACHKA1_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679437.1"
                     /translation="MYAEKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQTQL
                     SDVKDAAEEVKMQAAGKVAEVSEAAQSGLSEVKDTAPEKGAEISVTEE"
     gene            complement(8166..8354)
                     /locus_tag="ACHKA1_001775"
     CDS             complement(8166..8354)
                     /locus_tag="ACHKA1_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO5679438.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDEKMTQDECEKLFAPAKEP
                     GRYIGVRVEDFRCEVVKA"
     gene            complement(8732..9109)
                     /locus_tag="ACHKA1_001776"
     CDS             complement(8732..9109)
                     /locus_tag="ACHKA1_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO5679439.1"
                     /translation="MLCLKYPEPEVVSAVHPAGSVFVLPPQGAPGISCTTRDNLERLR
                     GHLAAQLGRVECIRCQPQRVGLNSSVAVMLEGQQGQYVHILLTVSGHESWPSEEEYIH
                     PRWYISVTDAADLFYLLLWLGEG"
     gene            complement(9114..11810)
                     /locus_tag="ACHKA1_001777"
     CDS             complement(9114..11810)
                     /locus_tag="ACHKA1_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO5679440.1"
                     /translation="MTRQDETKVYHACPSVIDFLPWVEYLDEEQCLLLDDGVSVGAVY
                     EVSPAATEGRTAERLEQIRDTVEDALQDSFDEYDSHPWVVQFFCQDENDVDTYVDQLR
                     GYVKPHAEGSSFTEAWLREMERHLKGIARPEGLFRDTLVTGQPWRGQQRRTRMVIYRW
                     IGKNNHDPMPPVAMLNQVCSRVVGALGGAGVRCTRMNGQQVHGWLLRLFNPRPEWVDR
                     DILYRMASRAEPQETPEGMMPVMTDFAESLWFTPPVSDPENGVWWLDGLPHAAVVVEK
                     LRTPPEPGTITGEQARGEKTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAV
                     GENSESLRAREDVATVKKYLGNRHKLYRAGITFLLRAEDMESLKRRRVELTTVLLGAG
                     LQPVRPEFDVGPLNSWLRALPMCFDPDTDKKQWYTRLMWVQHLAGLLPVTGRETGTGH
                     PGFSFFNRGGDVLTFDPLNKLDRTQNAHLLLFGPTGAGKSATLCGSLSQIMAVHRPRL
                     FIAEAGNSFGLLADYFESLGLSVNKISVKPGTGVCLPPFADAHQLVEQGETLQSVDEH
                     SLPDLDEDEGDEEEEKRDILGEMEISARMMITGGDPKEEAALKRADRAMIREALLMAT
                     HTTYREGRQMLPVDLQSALWEISRDTQRNDVRRAKAAEMAESLGMFTQPGSFEAELFN
                     REGKLWPEADVTLIDLGHLAREGYEAQMALTMVSMTNMINNIAERDQYLGRDILFVVD
                     EAHIVTVNPLLSPYMTKVVKMWRKLGAWLWLATQNLKDYPDVSEKMLNMAEWWVCLTM
                     PPEEVNNIARFKSLTEEQKAVLLSASKLSGCYTEGVVLAKRTEALFRVVPPSLYLALG
                     MTEKEEKAERRALMKVHQCSELEAAFHVARKLDGLRGLVGRE"
     gene            complement(11948..12340)
                     /locus_tag="ACHKA1_001778"
     CDS             complement(11948..12340)
                     /locus_tag="ACHKA1_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFO5679441.1"
                     /translation="MTVMVAVFILSGCSTSKEELLPPGEQSMLEIWQGKGSGGHSRHM
                     TAARDTLRRPLTMPEQQADAHGAISYSRTQESEISQQFPRLPNPDMVMYVFPHLASGT
                     SPVPGYSTVFPFYSRTQYAMPGERTEAL"
     gene            complement(12376..13863)
                     /locus_tag="ACHKA1_001779"
     CDS             complement(12376..13863)
                     /locus_tag="ACHKA1_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFO5679442.1"
                     /translation="MQIKSNTIVKYVVPCVVVAGILTGLKSCGGDTGEPSSAESSGTL
                     AGLSPDELKALGVEGDTPEDTLRTLIGRMNQVRERQLVLDKQNEKLLKENEMLRKRGT
                     DVSGQVSEAVAGLQKNYEQSQRQFRNEQNALMTQVQSLTEQLKRQQESADAGAGGQES
                     DIPAGLGLDGLGSVTGSAASPGTDGLMWVTPADQVQDEASSVGDKSGPRFPVSFLDDN
                     PLTRQKAAYEQQVKGYSNEHQKASTRAVYTLPENSTLAGSRAMTALLGRVPVNGTVTD
                     PYPFKILVGKDNLTANGIELPDVEGAVVSGTASGDWTLSCVRGQVNSITFVFADGTVR
                     TLPSSDVNGSSNAQAKNNSSTGGIGWISDDDGIPCISGTRKSNASTYLPTIFALSAAG
                     AAGDALSEGQYTSQNNVNGITSTLTGSAGEAALGKALSGGMNETAEWVKQRYGQTFDA
                     IYVPPGQTLAVHITRQLAIDYEDQGRRVRYDFTLPGEVHGTGGLD"
     gene            complement(13853..14761)
                     /locus_tag="ACHKA1_001780"
     CDS             complement(13853..14761)
                     /locus_tag="ACHKA1_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFO5679443.1"
                     /translation="MNGAERCLYRSIVMVLSLLVLPLSARADELMKWERIPLPVTLMA
                     GQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKAESVFPQTRLQLQDVESGEIIL
                     LDVTAAEKGPTEPVRIVYEGSVSTVSQAGTVASARSAEHKKVVPGGDDGSQALRQPVK
                     YAEPLPVLLTRYAAQSLYAPLRTLEAVPGIRPVNAHLPGKITTLYPSEPLTVTPLAAW
                     AAGGRAVVALKLTSTVSRKIILDPRALQGKFIAATFQHRWLGAAGTPEDTTTLYVVTA
                     ERPEQAFIAEPASRRIPSRGEVKHAD"
     gene            complement(14761..15423)
                     /locus_tag="ACHKA1_001781"
     CDS             complement(14761..15423)
                     /locus_tag="ACHKA1_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFO5679444.1"
                     /translation="MSRFRHALRDRDQHILTLRIFSGVLAGILMLVCIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILESDARRRSDAGELLERVRVVYEIPEHGYGGRYNKNPVTVEGRDNWQVQLDLV
                     ADEYFHAEPVKRALVRYPIKVVRMEGDPERNPFGLALDCYSSTPQRLEAASPAAGKGD
                     NQ"
     gene            complement(15420..15782)
                     /locus_tag="ACHKA1_001782"
     CDS             complement(15420..15782)
                     /locus_tag="ACHKA1_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFO5679445.1"
                     /translation="MQTIRFLPDRLNVEPAVFRGFTTPELGLAALTGAVAGVIWPLPL
                     IPLLGWVIIPTGMLLTPLLLVWFGGVWISRLKRGKPDNWLWQHLAKKSSAWGGGRKTF
                     IREAHGWSLRRTRSRGKK"
     gene            complement(15795..16109)
                     /locus_tag="ACHKA1_001783"
     CDS             complement(15795..16109)
                     /locus_tag="ACHKA1_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO5679446.1"
                     /translation="MAVFLCTGITPALADLPQIEPPTSGGGGGLFGQIKGYLQDGVVL
                     FGLVLSAVAFVKVAEAALSTFSEVRDGKAGWGQFGAIVVVGVVLLVAIIWLLGKSASI
                     IL"
     gene            complement(16186..16428)
                     /locus_tag="ACHKA1_001784"
     CDS             complement(16186..16428)
                     /locus_tag="ACHKA1_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFO5679447.1"
                     /translation="MAMTALQNTAFRAASGEVDLSVLHLITLGFLLAVVFLWAAWSLT
                     DAWAGWRSGDVRDGVFGWFMFRLVLLLICSIWMFAG"
     gene            complement(16428..16769)
                     /locus_tag="ACHKA1_001785"
     CDS             complement(16428..16769)
                     /locus_tag="ACHKA1_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFO5679448.1"
                     /translation="MFSFPASSCRAGRLAGAAGMLFLLSFALPAQAAEKDELATVMRQ
                     LDHVQAALERARTASLQASSQGERYFFDYLRARADLQTIRDGIDHYLAPSRAQPRSSA
                     AVTGDYRRERP"
     gene            16882..17448
                     /locus_tag="ACHKA1_001786"
     CDS             16882..17448
                     /locus_tag="ACHKA1_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679449.1"
                     /translation="MDEGKQPGTPGMNDIISIIILLTVYLIGLIFIILTINDIDRSSK
                     KRKYEKKLKEAIINGSLQNNDICLLAERWSASHEHIKSALLSVFNDFLTDNNFSHHVP
                     RVRELITWYEKYAPFTELPEDIKQQLQNIISLPDVSESEVQLLATSLHDIYNSCQKKE
                     KLARRINYVLGAVSIASFCISLFQILFK"
     gene            complement(17832..18593)
                     /locus_tag="ACHKA1_001787"
     CDS             complement(17832..18593)
                     /locus_tag="ACHKA1_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO5679450.1"
                     /translation="MAEPRREVNRAAQPSPPRQHGLLWNLLWGWPWMLVGILFSSLLL
                     SLLVEYIGIAFFWPEQGAAHSEAVMKTELGWLSSEFTRSLLLSAPSVTVTEWIATAYQ
                     WLFVETGFLSWLKQQNAGAVVGNSVSQYSEDVGRWLSHYLYEYLMATVYVTVVTLIRV
                     TILVLSVPLFVMVILIAVVEGLGRRDLRRYGAAYESSFVYHHAKKSIKPAIYIPCILY
                     LSWPTSVYPNLLIWPAAMLLGVAITVTMASFKKYL"
     gene            complement(18586..20682)
                     /gene="traD"
                     /locus_tag="ACHKA1_001788"
     CDS             complement(18586..20682)
                     /gene="traD"
                     /locus_tag="ACHKA1_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO5679451.1"
                     /translation="MSNRYVIEALLRPAVELNTAVVAATAAGVCVTAPWAVALAPSVS
                     YVTAAGFGVLAVVRFRQGMKIIRYRRNLRRLPRYVMSSRQIPVSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLQPGSLYRTARWLEMKTEHSLPWIGQLLRRDSPLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHTIVYGTTRVGKTRLAELLVTQDIRRGDVTI
                     VFDPKGDADLLLRVWAEAHRAGRGDELYIFHLGWPEISARYNAVGRFGRVSEVASRVA
                     GQLSGEGNSAAFREFAWRFVNIVARALVALGERPDYTLIMRYVNNIADLYIRYAGKVI
                     SEQMPELENAILNNMNVLGEADVPRTMQNQPDAVRIWAIEVALSSEAGKKLYDPILDG
                     LRSAVRYDRTYFDKIVASLLPLLEKLTTGKTAELLAPDYLDMSDTRPVFDWEQVIRKK
                     AVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYKHGMNAGLPGGNGTKNPVNLHCD
                     EFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARTGSAAKTAQVQGNFNNMIMLR
                     VRENRTAELLTTQLSQVEIYTKTLVSGHQDTADVTTGQDFTSSTQDRVGTVKVPLLEP
                     ADIVTLPKGQAFALLEGGQLWKIRMPLPATDADDALMPESIAKIAEAMRRNYHTGEGW
                     WNETPSVNMPEGEQNG"
     gene            complement(20679..21467)
                     /locus_tag="ACHKA1_001789"
     CDS             complement(20679..21467)
                     /locus_tag="ACHKA1_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679452.1"
                     /translation="MKGAFTERIQANAPAWAHEQLVAWDTAEFAAMSDFITEHYWTGQ
                     GSINVYRIVGTDHPQYAGMSWLELLERGKRMDINIPLLEKNPGYYTQAEQQHAGMGFV
                     STDGIHWYVSADGNHRSCLARFLFHLQGEGRTQLHNVAQSIYHTDQAFRSACREIHNL
                     AEPLSRHDVYLRLQIRRQCVSREDLACWKVDRFRTEAQLTVDDLHAGGHDGPLVYKAL
                     LLNAADAWREVMVLQRRLEALSASPEKALPRSWWRRLLQRGTRS"
     gene            complement(21464..21946)
                     /locus_tag="ACHKA1_001790"
     CDS             complement(21464..21946)
                     /locus_tag="ACHKA1_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679453.1"
                     /translation="MIMTLYRFVMLLLRLLIVWPSMLAGCLVMLYVWNNLNGGEAGMT
                     QRYATESSRWRDAPEGHVMVQKCSSVISVSGMYVDEQECPRTAISFGEAVAEDIRELK
                     MLAVVLFLGALFMEGVSSVILFRGCGSAAVSGEGRGSPAVMPWPDGIGISKVQERRAE
                     "
     gene            complement(21943..22536)
                     /locus_tag="ACHKA1_001791"
     CDS             complement(21943..22536)
                     /locus_tag="ACHKA1_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO5679454.1"
                     /translation="MMPLSATSAALSASPVLTACLVLSLLAVVLGPALWRSRRKKACV
                     RTRRHQRYRATASRVMKRLNELQGDARRLKYLRKISPYVFEELLLLAFEQQGMPVIRN
                     PSYSGDGGLDGQVVINGERWLIQAKRYRRTIRPAHIDAFGELLRRKGCRGFFIHTGRT
                     GNISRARLTLWPDIELISGQRLLQLLNGTLLSNKGES"
     gene            complement(22533..23051)
                     /locus_tag="ACHKA1_001792"
     CDS             complement(22533..23051)
                     /locus_tag="ACHKA1_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO5679455.1"
                     /translation="MKRERLFYVLSLLLFCTTAGAELKVIADLGGRDASPFFEAINRQ
                     PPVPASVVPSPSFPQGEAAMLPVSTPEMSPGEVVARRLQLPGIGALFLVGDDAHSRTW
                     LQMNAAVLRERKAAGMVVNVADESSLRVLRELAPGVVMVPASGSVLARRLQISHYPVL
                     ITDNGLSQVPAP"
     gene            complement(23048..23728)
                     /locus_tag="ACHKA1_001793"
     CDS             complement(23048..23728)
                     /locus_tag="ACHKA1_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO5679456.1"
                     /translation="MARGGILTVLLMVACSGVCRAAVQNVPDGYVRVARAHGIPAELL
                     YAVSLTETSMSPEAMSGVLKQRRRDFRQPDVARPWPWTINVAGKGYRYATRLEAWQAL
                     QVFLKHYPAKRIDVGIAQVNLGWNGHRFPSTWAAFDPYTSLNTAAVILQECRRRHPVS
                     WLQVAGCYHHPAGGAPAARYTAVVRRHLARLHRDNFLNPSDVHVAATAMLTPSPRYIW
                     TEPQEELK"
     gene            complement(23707..24426)
                     /locus_tag="ACHKA1_001794"
     CDS             complement(23707..24426)
                     /locus_tag="ACHKA1_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFO5679457.1"
                     /translation="MKRKILYLLALCPVLAQSAGQTNMSSEAQRTTGTVVTQSTTSGL
                     QQQAGQWGVSEEDYRRYQSLMSGPRGIQSPGLDPLTVLGIEARTPAERRVWAEKWVRA
                     EYERTEKELAFQQEVNAAWKRLYPGMLPVSMGKTGALTGDTGGRLALFVKAKECGTCD
                     ARLASVLATGRQVDIYLVDSQGKDEILRQWAHAHSIPVEKVRSRHITLNHDAGRWLRF
                     GEGKMPVVLQQGADGWRVAAF"
     gene            complement(24430..25179)
                     /locus_tag="ACHKA1_001795"
     CDS             complement(24430..25179)
                     /locus_tag="ACHKA1_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679458.1"
                     /translation="MSEKQPQIPEGASGMSPERRKSRFPGVLILSVTVGLVMGAVVYG
                     VLMLMTGLDSRITTLEGQRREMLSASELAPLRMKLTETEKQLKDMEKRLAVLEQQPVL
                     TASWKEQLENLPEMLEQIESARKKLSLRLDTLSGLVEKMQKVPESPSQPDSRKAGMKK
                     TEEKKPAPVTSSRKSRAVHTVRQAPFVLTGTELRGGSTRAAVAPRGYTSLSQVALLGE
                     GESVAGWTLVYAGHGEATFRVNGRQTVLRVE"
     gene            complement(25176..25775)
                     /locus_tag="ACHKA1_001796"
     CDS             complement(25176..25775)
                     /locus_tag="ACHKA1_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HFO5679459.1"
                     /translation="MKKRLILSGLTVCLLMAGCQGTQKLQQRDAAMPVPSVTVNRNVQ
                     PASPDVYSQAPEVVRYDRYLLVSTEPQAVQRDPLSQVIDIRIPSSLKPTVADAMRYAL
                     KQSGYTLCATGPANGVLYRQPLPAVQYQPGPLRLRTALQLMAGPAWQLEVDDVQRVVC
                     HSLRDGYQLPVSQLAPVPVTVPFVGREPGVKATGGFLKK"
     gene            complement(25961..26881)
                     /locus_tag="ACHKA1_001797"
     CDS             complement(25961..26881)
                     /locus_tag="ACHKA1_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO5679460.1"
                     /translation="MNENTPTTPTPHDAAFRAMMECPSVARDFLKGALPPEQLNRCDM
                     NTLQLEPTTFVDPDLRQFACDVLWSMKTTDGRDGYIYTLTEHQSKADKYMALRMMRYV
                     LAVIHRHLKAHACAPVVIPVLFYHGVPSPYPYSMNWPDCLDDPAFGRELYGEGRPPRV
                     IDVGLLDDEGIRRYKQMAALMLMMKLRQKKVDLMVHLDFLSQLIQIQETHTQVVTLFN
                     YMVKECDSASPEFIRAMAERLPRHEDDLMTIAERLELAGIEKGRQEEREKALQENYAS
                     ARRMQQMGISSEKIKQILQFSDDDLRNALR"
     gene            complement(26930..27490)
                     /locus_tag="ACHKA1_001798"
     CDS             complement(26930..27490)
                     /locus_tag="ACHKA1_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5679461.1"
                     /translation="MASRGVNKVILIGHLGQDPEIRYIPNGGGAVANLTLATTDTWRD
                     KQSGEMKEHTEWHRVVIYGKLAEIAGEYLRKGSQVYIEGQLRTRKWFDQQNGVDRYST
                     EVVVSVNGTLQMLGSPRQQQGGGNVSSAPAQGGWGQPPQNAQQSTGKNGQASSGSGGM
                     PPMDFDDDIPFMGRGYGVERRIIHVI"
     gene            complement(27506..27709)
                     /locus_tag="ACHKA1_001799"
     CDS             complement(27506..27709)
                     /locus_tag="ACHKA1_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679462.1"
                     /translation="MKPTSGILAAKAGYTLPLQILSSARGYYIGTQCDEGPVSRESEE
                     YFRNYEQAVRALENGIWTQRECV"
     gene            complement(27800..28267)
                     /locus_tag="ACHKA1_001800"
     CDS             complement(27800..28267)
                     /locus_tag="ACHKA1_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFO5679463.1"
                     /translation="MTANTSSANAKTEYFNLTLKGLGYLSNIRQVNHQNGTFLSCVVN
                     ALSGPTDSPSYVRFDVTVAGKEATSLIARCQKAVDEDRKVLIGFTMSNPSTDIFTLNR
                     GEHAGEQRVSLKARLIKVEWIKIGQDMVYKTEKSESIPPQNGNTPREYAENSF"
     gene            complement(28946..30988)
                     /locus_tag="ACHKA1_001801"
     CDS             complement(28946..30988)
                     /locus_tag="ACHKA1_001801"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO5679464.1"
                     /translation="MQLFLCEKPSQAKDIARELALSKRGDGYIGDDNIVVTWAIGHLL
                     ELAEPEAYGEQFGSPWRSDVLPLIPEQWQMVVKPKVKKQLIVIRGLLKRASEVVIATD
                     ADREGEVIARELLEYCQYRGRVRRLWLSALDEASIRKALNNILPGEQTTLLSEAGKGR
                     ARADWLTGMNLTRLYTLKARERGISGVLSVGRVQTPTLAMVVRRDQEIATFVPVSWWQ
                     VIVWLEKEGLRFRSNWVAAEQYCDDEGRCVNVQAAGAVLGLCRQQSSAVVQDVVQKRE
                     KTPPPLCFSLGTLQEACSRKFGMGAQTVLDIAQSLYETHKATTYPRTDCGYLPASMHE
                     ESSEVLEAVGRSDPSVLPQLAELESGRVSRIWNDKKITAHHAIIPTRQVFDLSSLNGD
                     ELKVYQLIRQHYLAQFLPEQEADVTEATFVIGGQLFRSRGRVNVIIGWKQLFPPENHA
                     DEEKPDSDESGNILPPLIKGELCALDGAEQKELKTVPPRPFTEGSLITAMKNASSGVT
                     DPALKKILRENAGLGTEATRAGILETLFARNYIERAGKHIRSTQLGRELVAALPDSLT
                     NPGMTALWEQALDDVAWGKISLEAFMEKQHQWIRHLVLSGRNQTVNLSVESGPSCPLC
                     NGPTVKRKGKSGFFWGCSRYPDCRGIAGEQGNSRGRKRKINFSHKQKSGDVGDKLN"
     gene            complement(30991..31695)
                     /locus_tag="ACHKA1_001802"
     CDS             complement(30991..31695)
                     /locus_tag="ACHKA1_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFO5679465.1"
                     /translation="MPQEKTGEVRKSEQVGALQSSLTIALHTRYAIRLWEGRRNNQKE
                     EKQEKRPDIISMPRAIAFAGQATRDSARDNPYADMMLVRLENALTKATETIEKHLAWL
                     DGILKNLPGQISLSDIKSSSPVNIGVYSSSPVGYRCVWLLIGYDRLVMKVFQVFHYGL
                     ISRTKRDELLNQGGYAVRRIYGIIQNYKSVSVTRADILSGTARGKEAVNRMGEPDPDV
                     MSGKKRSVYSPPVKAG"
     gene            complement(32489..32968)
                     /locus_tag="ACHKA1_001803"
     CDS             complement(32489..32968)
                     /locus_tag="ACHKA1_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679466.1"
                     /translation="MQKYKQESLRAAMKESGLLIAKICACVIAGVVVIWGLVTGTETL
                     LTALKTMHEQYDNSMCYIVCATGWFFLVLSNWLWVTYSLYKARTGSTVGGGLQFMLLL
                     SLLAECFSFFVIFWSLSHLDITDIQLLVMGITTLMLFLSLNVKVVVIINHVASGNSD"
     gene            complement(33080..34279)
                     /locus_tag="ACHKA1_001804"
     CDS             complement(33080..34279)
                     /locus_tag="ACHKA1_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFO5679467.1"
                     /translation="MTLPVDSLIAYTLEKMQERIEQKGRQQDGKIQSGLLFTGNVHDA
                     IPRRLLLDTRLSPLDKMAWMMIRLYAQNNEGAVFPTYDELQLQLASPGKGKASRETVS
                     RVLLMLRITGWLSLCKRVRDEKGRVRGNIYAQHDEPVTCRDAEILDPRYLDMVADACM
                     SRNRTISQTARDILCEIKNDPGMRHYHSHISQLEARIGSAQTPGQMAARQRVTEDRMQ
                     PGSETEPGVETQSSVLKMLSSDSEPSEKSEGYHRVRKPNHYVRNITHSVIKNTYVLPE
                     ALTGQIREEEAIMLTSQLQALPEELAQGVLESLSGMLGSHSLQNPVGWLLAVLKRARE
                     GNFYPPKQKQRVVVPASVLAGERGRATAQSVSSGKRTEPISAERLSGIVAGLRASLRR
                     CSAMRSE"
     gene            complement(34297..34764)
                     /locus_tag="ACHKA1_001805"
     CDS             complement(34297..34764)
                     /locus_tag="ACHKA1_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679468.1"
                     /translation="MAIAERTGISSRGDTYEIWLGANCYHYRSDGYAVLLKEGRNRAY
                     PCRVTSETIKSALAAIVELQQKLTAAEKKKECENHGFLLPAEMLIRCLEDQTGGRWIR
                     AHEPVMLMKRLISNLKKARPECTVPDEALAYLRRNCLVNMGDIPVTSRRMSDV"
     gene            complement(34768..35181)
                     /locus_tag="ACHKA1_001806"
     CDS             complement(34768..35181)
                     /locus_tag="ACHKA1_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HFO5679469.1"
                     /translation="MAEINFQVLREAEQLTGVATAGRPVARHFDDGLTLEVNRTCTTG
                     ERNANSALYGAVWRAAKAMGYRRCITYTRADESGASLRAAGFVRVKELPSGKSWAESS
                     VALKSTRDPVGSGGVPCVLREIRRMSTTVIHIKGE"
     gene            complement(35196..35804)
                     /locus_tag="ACHKA1_001807"
     CDS             complement(35196..35804)
                     /locus_tag="ACHKA1_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFO5679470.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGLDVMML
                     AAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKAE"
     gene            complement(35801..36526)
                     /locus_tag="ACHKA1_001808"
     CDS             complement(35801..36526)
                     /locus_tag="ACHKA1_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFO5679471.1"
                     /translation="MSDMSHQERLIRRVRKLLELSRNNSNAHEAGLALARAQQLMAKH
                     GITEQDAGLSSVQTSSSQGAPSDAEKVPAWMFRLVMVVTQTFGCRAYYDWRAVPGGRY
                     RRCVTFYGFSERPQVAAYAFDVLCRQLREATDAYLRTQSRRLKLSTRRARAAQFRDGW
                     VQGIRQVVMPFSVTEEESRLMRLWMEQQDMVTVDSREMKACRGGDLARWQGYEAGKNA
                     SLHHGVGESARQVVPGLMIREAS"
     gene            complement(36519..38252)
                     /locus_tag="ACHKA1_001809"
     CDS             complement(36519..38252)
                     /locus_tag="ACHKA1_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO5679472.1"
                     /translation="MAHKPLDLGAAIMQPGRQTTGAGNVVAMGETPMVLTLDQLRPNP
                     DNPRTTRNPRYDDIKNSIHARGLDTVPKVTRMPDSEPDVYIFSDGGNTRYQILTELWK
                     ETGDPRFYRIHVLFKPWPGRLQCVIGHLAENEVRGELTFIEKAQGVHKARQIHEEQLG
                     RTVSLRELADLLTHEGLPIHNSTISRMEDALKYLYPWIPDLMESGLGRPQVTALLALR
                     QDAERVWGQFAVAADTDAEFDRVFGESCRKFNSPELWSLEMFRDELIGDLLQALPHPS
                     LDYDRWLLELDPKERNRRHHFGEPEPVVVPPSVRGSSTVQPGAPVSGIRSEVPECQPD
                     TDTEAMPESTVSIFPGTERKQHGITRPLKAVSDADTERTEKCSRPSQEESAGRGDCGA
                     SSAENALRPEIQPDMYGGKPVFSGDVMEDDAAGAVLASVMTGDTAPGTTDALPASVTH
                     FAVRRDDIEHLQNEAFRLAWELAESAGCAEEIAMDRESDLSAGFGAAVEKCSPVVAFL
                     AGLAGDAPRTVLPVSLKDLLTGGSAPEAWPLLDDDHAVKLLRLLSVLRRLRALQRSVL
                     PDDDEGDENDE"
     gene            complement(38252..39619)
                     /gene="dnaB-PI"
                     /locus_tag="ACHKA1_001810"
     CDS             complement(38252..39619)
                     /gene="dnaB-PI"
                     /locus_tag="ACHKA1_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPI-7-type island replicative DNA helicase"
                     /protein_id="HFO5679473.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTALQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLEL
                     SMCLSAALRRTEESVLVFSIEMPKEQLILRMLSMLGRVELNHLRSGNMDDEDWLRVSS
                     AVGMALGDGGMGSLGERIIIDDCCEQTPSSLRAGARRYTRLYGKPSLIMVDYLQLIRA
                     PELENRTQEIAEISRSLKALGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALE
                     QDADLIMFIYRDEVYNPDSLAKGEAEIIVSKQRQGPTGTVRVRFDGRYTLFTPSENDG
                     PGGYA"
     gene            39816..39983
                     /locus_tag="ACHKA1_001811"
     CDS             39816..39983
                     /locus_tag="ACHKA1_001811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679474.1"
                     /translation="MNTEKQLPSGIESEVKYTLQFWLLSNGKVMTEKDGLRNPFPPNC
                     IESAHPFTPFQ"
     gene            complement(39967..40869)
                     /locus_tag="ACHKA1_001812"
     CDS             complement(39967..40869)
                     /locus_tag="ACHKA1_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679475.1"
                     /translation="MSSSVKYSPVPAVLTRPELLAELNLLMTRIASGCRVGLPASGRP
                     VIAVHGGYVSYSSPRTGHVFQDGSRPETVMTVSNRERMCEWFSQVWLPLHLAALEGDE
                     NAFFSAEVNMVRRELQSVPGNSVNCMCWASLTALAFDAEERVERQKGNNENIATYDES
                     IATYAFRAACTVSRCLSRNASGGIPRSYPEWKAILFPRDGADIFPDGWLENMTRRAER
                     VFTSAKRQCGMLSASQAALFSQQTRQCASEQCTGRIQAGRHHSGSHPDVSVYTERCAQ
                     GMSLSPVHPTRQCPVQSTDSTETE"
     gene            complement(41105..41314)
                     /locus_tag="ACHKA1_001813"
     CDS             complement(41105..41314)
                     /locus_tag="ACHKA1_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679476.1"
                     /translation="MCVGCGYSGVRDGAGKNAPVWVKVNPESGLGLCSGCDAFLLQER
                     QRRQRHDQLFSCGLSCKQRKTEGAV"
     gene            complement(41647..42534)
                     /locus_tag="ACHKA1_001814"
     CDS             complement(41647..42534)
                     /locus_tag="ACHKA1_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO5679477.1"
                     /translation="MKILPVISTKGGEGKSTQSANLAGFLADAGLRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLSHPDNIISRTAVRGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFQTYDVIVVDSQGARSVMLEMIVLSATESVVGMVNPVLPDVR
                     EFIRGTVNVMENLMPYRELGIPLPKVRTLVNCMDYTALARQTLAELTGIINAGRYSKV
                     LPEGAVTLLSTQIYDLNIYKQGHAAGQPVHRLEKVSARCSDSALVTMHSLACELLPEW
                     REFFDALLENGGKSLWAEN"
     gene            42850..44034
                     /locus_tag="ACHKA1_001815"
     CDS             42850..44034
                     /locus_tag="ACHKA1_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5679478.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQVTDILQQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYIDGRREMMQWYADYIDSLSEQ
                     VS"
     gene            44363..44944
                     /locus_tag="ACHKA1_001816"
                     /pseudo
     CDS             44363..44944
                     /locus_tag="ACHKA1_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            45031..45309
                     /locus_tag="ACHKA1_001817"
                     /pseudo
     CDS             45031..45309
                     /locus_tag="ACHKA1_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(45577..46581)
                     /locus_tag="ACHKA1_001818"
     CDS             complement(45577..46581)
                     /locus_tag="ACHKA1_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO5679479.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKVLLRKKFTRTKLIEFLAG
                     CASSTVVMEACAGAHFMARRIADLGHEAKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEAQQAMRALHRVRESLVSDKVKTINQMHAFLLEFGISMPTGVAVVKR
                     LSTVLAENELPPYLAQLLMRLHAHYIYLVGQITELEIALEQELSRDEVGQRLQTIPGI
                     GPITASVLSSQLGDGKQYSCSRDFAASTGLVPRQYSTGGKNTLLGISKRGDKNLRRLL
                     VQCARSFIQRIENQSGRLADWVRALLARKHSSVVCCALANKLARIAWAITTQQTVFER
                     "
     gene            46630..47585
                     /locus_tag="ACHKA1_001819"
                     /pseudo
     CDS             46630..47585
                     /locus_tag="ACHKA1_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            47625..48104
                     /locus_tag="ACHKA1_001820"
                     /pseudo
     CDS             47625..48104
                     /locus_tag="ACHKA1_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            48247..48476
                     /locus_tag="ACHKA1_001821"
                     /pseudo
     CDS             48247..48476
                     /locus_tag="ACHKA1_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(48463..49965)
                     /locus_tag="ACHKA1_001822"
     CDS             complement(48463..49965)
                     /locus_tag="ACHKA1_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO5679480.1"
                     /translation="MNIRKLFCPDNTPRILLFLFFFVISVITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQRYEQLSQHRADSWKILPGAPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMIGSHPNPCDRLNSWPNHYLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQ
                     WLTGIRTENIPPFNPLTDEDNEPVMVFNGERNVPADSLKPQPLILPVRGK"
     gene            complement(50127..50888)
                     /locus_tag="ACHKA1_001823"
     CDS             complement(50127..50888)
                     /locus_tag="ACHKA1_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFO5679481.1"
                     /translation="MKKIIAVSALAVVGMFSGLALADRGKTGFYMTGKAGASVVTQTD
                     QRFRQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSRY
                     TLDTWYSPMVGGGREDTQNRLSVNTLMVNTYYDFRNSSAFTSSAFTPWVSVGLGYARI
                     HHKATYTDTSWNKSGEVSNFSALHYPGYDNNFAWSFGAGVRYDVTPDIALDLSYRYLD
                     AGDASVSYKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            51678..52721
                     /gene="subA"
                     /locus_tag="ACHKA1_001824"
     CDS             51678..52721
                     /gene="subA"
                     /locus_tag="ACHKA1_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFO5679482.1"
                     /translation="MLKNLRLHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKEPEALYIHGTAMASLIASRHE
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWSELLSRMGRNNERLIVAAVGNDGADIRKMSAQQRIWPAAYHPVSSVNKKQDPVIRV
                     AALAQYRKGETPVLHGGGVTGSRFGNGWVDIAAPGQNITFLRPDGKTGIGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYPSLADKVTEGRVLNAEKAISMFCKKN
                     YIPVRQGRMSEEL"
     gene            52738..53160
                     /gene="subB"
                     /locus_tag="ACHKA1_001825"
     CDS             52738..53160
                     /gene="subB"
                     /locus_tag="ACHKA1_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFO5679483.1"
                     /translation="MTIKRFFVCAGVMGCLSLNPAMAEWTGDARDGMFSGVVIDQFHT
                     GQIDNNPYFCIEGKQPGGSSISACSMKNSSVWGPSFSTLYNQALYFYTTGQLVRIYYE
                     PGVWTYPPFVKALTSNALVGLSTCATSTECFGPDRKKN"
     gene            53361..>53614
                     /locus_tag="ACHKA1_001826"
     CDS             53361..>53614
                     /locus_tag="ACHKA1_001826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679484.1"
                     /translation="MWWLVTAPVRELARNALLMAVWRVSLHKTLLYYVYQRVTRLEQP
                     VQKCPRSEHGKFSPYNGFLTPLLFSFSHINDGVYSDGRVFR"
BASE COUNT        12954 a        15626 c        12347 g        12687 t
ORIGIN      
        1 gcatatctgg tttttttatg atatgccgtc agggtgtgct tccttacata tcacgccctc
       61 ttccagacgt cgctggtcgt cgccctctgc gtcccttact ttcagcccct ctgagcataa
      121 tcgcccctcc agaggagccc atcacaacga caatgccagc gggttaattt tatctgaccc
      181 ggccttcagc cacatccttc acacgtttaa ctaatggctc aataaattta tctttcagat
      241 gccccggtgc cggagatgca tacgttatat atatccgact tcctttgcca tttttttcaa
      301 gttcaagcgt cagatgatca tcaaactcgc caccatccca gtcactgccc attttataat
      361 aactaccggg catggcatcc cacatatgcc cgttctgact tattgctgct gcggcccact
      421 tcccgttcgc atgtccgtta ttacgaatcg catcgacttc ttcatttgaa taaaacttat
      481 agtaccgttt cagtcttccc gcagctgtat ccacatcaac agggacttct atgacatgat
      541 cacgtttctc tctttttgcc cggggtgcct gagacagata tttgagatca tcatctgcct
      601 ggtttacacc tacgttcagt acttttgtca cagcaaatgc accgtcactt atctgcctgt
      661 tcagctctgc aatttcctgt gcagaacagg cactcagcgc aacacacgaa gcacaaacga
      721 gtaacatttt tttagtcagc atgtcttcat cctcatcagc agctcttcag tggataacat
      781 ccacagttac acctgcattt acgcgcaaca aaaacaccag atgaagattt tattcattca
      841 gctccgctaa aaattaatct tgctgtggaa tatcgggaaa acaggaagtt acgttaaaag
      901 ccgaaggaaa aactcacaat cccttcttca atctacatca gtatcatgcc caaaccagac
      961 gggttaacgg cagcaaagaa ccttgcagaa gcgttcgagc attataacga atggcatcca
     1021 catagtgcgc tgggttatcg ctctccacgg gaatatctgc ggcggcgggc agtaatgggt
     1081 taagtgatac cagatgtctg gaaatatagg agcaaatcca ccacgcatgg agcctgttaa
     1141 gactctcact ctcatcattc tcccttctga gtcccaacga aacgaccaaa acgctgagta
     1201 aagcgggcaa cattgccttc tgatgccacc gttctgagtt taccggtata gaacggatgc
     1261 gatttggagg aaacgtcaat cgtcacatac ggatacgtca caccatccag ttcaatctcg
     1321 cgatctgttt tgatggtaga acctatttta aagtactcat ccacactcgt gtcatggaac
     1381 actactgtgc ggtattcagg atggatattt ggcttcatct ctcttcactc atgttataac
     1441 ataacaataa gatacattct cagctagatc atttcaacag aatccccgag cccaagggta
     1501 atgtggcatc gcagcctctc atcaaatgga agacatgtga ttgcacattt atcatacaag
     1561 acgtatgata acactgtgtt atttatcctg ctgctgaggg tatagatatg aataacgaat
     1621 ctgaaaaagc aatgtggcgt tgtaagcctg tggataatcc atggaccttc acttccctgc
     1681 tgaaaaaagc agtctgtgcc gttttaccag atttcacacc tgctcaagat aaaccccaaa
     1741 atatgttttc actaagtaat gaaaaagata ctgattattc agtaccaatg agaccctttg
     1801 cattatatga cccgtggaaa catcaccatg acaatgctga tttctaagaa aagcaagggt
     1861 ttccccaatt atcccagcta aggaaaccct aagaatttta cttattagct aaatctccac
     1921 tttttgctgc tgccttagat acattacttg ttcctttgtt aattgcatcc cccataaccg
     1981 ttccgacact taccccagcc caggacaacg ctcccagcca cactgccggc aagacgagaa
     2041 acatcgtccc catcaccaga ttcattatca gatcatccga tgtgttctgg aatcccatca
     2101 tattgaaccg ggtatgtgta tccgaactgt acagcgccgt cagcatccag ctgtcaagcc
     2161 accgtgcaag ctcccaccag aatgtcagaa agtgtaccgc aaacaccaca aacgtcagtg
     2221 tgattaacgt cgacagttca tatgccgcaa acgccagaat cagcggtatc atgatgtaaa
     2281 tcgccatcag tattatcgac tgtaccatcg gcagtgcctg ccgcattgca tccagtgccg
     2341 gaaacgcacc cagtgctgct accgcactgc ccgccgttcc cgcaatccgg gccaccgcat
     2401 tcatggtcgt catatctgca ttgccaccat agccacgata cacctctccc ccctgagaga
     2461 gcgtcaggtt tgccggactc accagacgct tgatcacctc ctccttataa tccacctgac
     2521 tactgccaat catcttcatt gcagctgaca gacgtaacca gtaatgatca ctgacctcct
     2581 ccatgacccg ttttttcagc cccacactac tgtcactcca ccactcccgg catgtcggat
     2641 agcctccccg ccccgtatcc ggtcgtccgc tgtcacgtgc tgtattccac gggaacgcag
     2701 cccgcggcat ctttgactgc agcgtcccgt aatccccctg catgaatgtg gcgctcccta
     2761 tccaggagat atcatcaagc acttttttat ctttactctg cccctgatca cgctgtttcc
     2821 acatatacag tgccagtgaa taacagtcgt tcgtaaagtc ctgcagctca tcagcaagtg
     2881 ccctgttttc tataaacgtt cgctggatct caaaacgcaa ctggcgcaaa tcaggccggc
     2941 acggaatcga agccactgcc gcctgggtga cacctttcga caatttatga accaccaccc
     3001 accataccgg ggcagcagca gtctgatccc ctatactgct gatcacccca ctgtaccccg
     3061 tctctccggg ctcttcaggt gtccataccc cacaggtctg tgccctggat ttatcgaatg
     3121 acagcgtatc cagcgtcacc tgtaccagag gaacacagca ggatatcatc acaaaaaaag
     3181 caccatagag aacatgctca atacgcggca gggaaagcat ccctttgttt ccctcatccg
     3241 caccttcttc cctgactttc agccagatcc cgatgatcct gaatgccagt ggcagggcaa
     3301 acagtcctgt gctgatcaac acattccaca gaccgttatt caccacccag cccagcagag
     3361 tcagaaaata ttccagatag ttatttgtgg tcatgattgc tcaacgcccc ggctcagcaa
     3421 aaatggcgta ttcacacagc aggataaaca gaacactgac tgtggccata cgcagaaaag
     3481 aagcccggta ttctgccctg aaccccggcg catgccagat cctccacccc agccatccga
     3541 tcgcactgta aagacacaga cgccatccca gccagccata acgtgtctca tacatccact
     3601 cccgcagggc cgcaaatcct tctgcgtgac gtataccggt atcggccagc cagagggaaa
     3661 acacggtaat ccctgtcagc aacagcgtat acagggcgaa taccttcatc ctctttttca
     3721 cccggccata aattctgctc atcctctcac tccttatccg gggcatcgag agaatggata
     3781 cggctgtcag cattgtcagc ctgctggatc atcgggttat tccccacacg ctcgttatcc
     3841 cgttcaatga tggtcagaac actgttacgc gccagatccc gcttcagctc catctcgttt
     3901 ttcagcgcca taatttcccg atccagcgcc tcaatacggc gttccccctc ttccagtgca
     3961 gcaggctgag ctgcagcata cggctcggcc atccccgtga gcagcatcct gcgcatcagc
     4021 aacgccgttt caacggtatc cgccatggcc agttctcccg ccagacgcga cgtcagggca
     4081 ccattgtccg gatcacgctg cagggcacgg atcactcccg ctgtcacaga cagactcccg
     4141 gctttcagtt ttgccagatt cccggctgtc ggcttgtcac gaccattgac catacctgcc
     4201 agcagttccg tgttaacccg cgtcgcttct tcaagcatcg gcgcaaaccc cgtgccggca
     4261 atactggtcc ccggctgctg atcagcatcc ccgctggtac atgcctttgc atcaccacag
     4321 gtccgcatgg atttatcccc cagaaccttc accaccgccc gggcagcttc ctctgcatta
     4381 cggtatttca tgcaggcact cccgttacag ttcccgggac tgacactgga tgtactggtc
     4441 gaaggcagac tgttcatcat attgaaaccg gcagctgtca gatcatgtgt cggccggata
     4501 gcagactgtc ccgctccacc acgctgctgc cctccgatcc agcgttgccc ctcctccccg
     4561 gtaacctgac gacctgcttc atcccctttg atcgcatcac cgtcccccgt attcacaatt
     4621 tttttgtact cctcgagcat ggccgcctgc gtccacttat tgctgtccgt gaactccatc
     4681 atgcgtttcg ccatgttctg acaattgagc tgcgctttgt cgaatgccac attcgcctgc
     4741 agtacaccgt tggtcagcat gtcatacagc cccggattag cccgctgtat caccattgcc
     4801 ggcagactcg ccacggctcc gctcgccccc tgaatcacat cccccatcag gtttttgaaa
     4861 ccatcagtga tcccgttcag ctggttaccc actgttgttt tcaggtcaaa attgccacac
     4921 atcagatcac tgctccaccc gagatccacc ccaagtttct gcatattgct gcgcgttgcc
     4981 ggctgcgata tcaccgagcc ccctcccagg gtataaaaca gtttgtctga tactgcgccg
     5041 tccacggatt taccgtagcc aaacgcactg tcattcactt tcggcaggga caaaaagttt
     5101 ccgtcagcct gtaccgtacc cgcacaaagc accacagcca gaacaggtat cgtcattttc
     5161 tgtttcatca cttcccctta cttaaaaatt cgtactgccc aggaatgtct gtccccgttt
     5221 cttgcagcaa ctgtagggct gccacagggc aaaggcagca ttacctgttt cagccgcttc
     5281 atgctctccg tcagggaaga ctgcgcagga cgtggagact gaaggcgtca gtctctgcca
     5341 tttatggttc cgggtgcccg tattttcctc caccggatcc ggtggccagt aaccgtcctt
     5401 cttccctgct ttcaccggct gatagacatg tggttcaccg gtgcgggtga taatgtcagc
     5461 cacccgctgg gccaccaccg cagcagcttt atcatcatct gtctgggtca cgaacccgga
     5521 gcggggatac acattgcccc acatattctc ttcttcctgg ctgcctattt cgcgtttacc
     5581 ggggatcagg gcctccgggt acagtgactc cggtagtcct gtacgccagg caatactgtc
     5641 aagcgtactg agaaaataag gaacaaaagg tgtcaccggt gactcacagg aatatcccgg
     5701 caccatccct ccgataagcg tggtggccgg gtgccctgtt gcatcggcat atttaaaacg
     5761 gattgacgaa cgacgttccc cctgtacctt catgtctgcg gggttccctc ctcctgctga
     5821 cagaccggca atacctttgc ttatggcatt ctccggacca ccggctgtac tgctgaccgc
     5881 cgacatttca cgccagggat ttccgccagg tgcggtatag gtggaaacaa ccacttccgg
     5941 gatataatgg gaaactttca ctgatgttct gaccttgcaa ctgaatttgg tgcagaacag
     6001 ccagtagcaa atcccgctga cccgccagct gatacagctc tgtgaggctg cactggccac
     6061 tatctgcgcg gtgttcagcg atgcctctgc caccggcaat accgccatac ttaacagcag
     6121 aacaccggcg cgatgacgtg atgtgcagga aatcatggct gccctccccc tgtacgcagt
     6181 tgttctgcca gcaccacgtc tgtggttccg tagaccactt cactgtcatc aaaaaccaca
     6241 gccggatatt ttttcagtcc aagggaccag gcatgtgcta atgcacggta gtgttcagca
     6301 agccctgcct gaaacgactg ccattcaggc gactgcatcc gttccatgac acgggcagat
     6361 gcttctgcag gctctgcggg aagtaccccg aaaaatgtat cctgcgtctg ctcaggagca
     6421 tcaagcagga caacacgaac acctgctggc gggttcatgg ggcgctggtg cgaatcggta
     6481 taaattgttg taccggcaga tgcagccacc ggtagtatca ggaaggaata cagtaacagt
     6541 cggtacatga tgcgactccg tcagacaact ggtatctgac agcgtgcgca aaggcaaagg
     6601 gacaatcagc aataaattca aaaccggatg ataaaaatta agcctcttcc tctgaataac
     6661 gcacactctg catctgaatc aaattaatcc ggttgcatca caggaagcca ctgattcggg
     6721 gtaataccat aataacgcct gaacacagaa ataaaataag aagcactggc atagccacac
     6781 aactctgcca cccgggaaat acggttttct cccgcagaca aaagctgagc ggcatactgc
     6841 attctctctt tcagaagaag ctcactgaag gtcgtcagct cttcctgaag ctttttcttc
     6901 agtaaactct cacttatata taacagactg gccacttcac ccagcatcca tggacgggac
     6961 aaatcagcac tgataatatt cttcacccgg acagtgcagc tccggataca ccccgtgagc
     7021 agggcgtgga aatccggatg ttcctcaaac agcgccagac tggaaaaagc caccgcatcc
     7081 ctgaagtaca ccgacgatgg ttcatctgta ataagttgtc tgatcagaca ttccgccagc
     7141 acggacatct gtggccggat ctgtaaaagc aaagacgacg ggctgactgc cataccggat
     7201 aatggcgtgc agaaccgcag ataccgcctc accacctcct cactgaggtc agcgataagg
     7261 aaacgtccgg tatgagcctg aaaagcaggg cgcagatgcc cggcaatcag aagcattgtg
     7321 ccggcaggta tgacagagtt cctgtgctcc agacaaagca ttgtctgttt gagagccata
     7381 atgacaacgc aggtacaggg catcggatat ccggtaaata tgttgattta ttaaagagac
     7441 agacatgccc cgtatataaa attccgtgtc accgggtgct gaaaaaaact gcaccgacgg
     7501 cgctctttta ctcctccgtt acggatattt cagccccctt ttcaggagcg gtatccttca
     7561 cctcgctgag cccggactga gccgcttcag aaacctctgc cacctttccg gcagcctgca
     7621 ttttcacttc ctccgcggca tccttcacat cactgagttg agtctgcgca gcttccttta
     7681 tatctgccgc cttcttactt acattctcct ttgtcgcatc atcacagccc gcaaccagaa
     7741 atggtgcgga aatacataac gccagaattg ttttttcagc atacaatctc tcattattaa
     7801 gtgttaacgc agaacagctt atatttcgtc cccgggaaat ggtcaaaaga caaccagaac
     7861 atcacagtgt cagggcacac tcccggtata tgcttttata accataacat gctgttcctg
     7921 atattaccgg aaatgtgccc actacattta acctgttttc agcaccgggt catcggctgt
     7981 gctgtatttt ttattcccgt cacagaaaca cactattacc gttgatttca tgacgaataa
     8041 aatgaataaa ttctcccgtg gtctttacca ccattattat accaataaca ccatttacac
     8101 agatacactc cgggactatc tgtttttgcc ccctccgact ccgccggagg tttttatttt
     8161 catactcagg ccttcaccac ttcacaccgg aagtcctcca ccctgacgcc aatataccgt
     8221 cccggctctt ttgcgggcgc aaacagtttt tcacattcat cctgagtcat tttttcatcc
     8281 tggtaacgca gccactgaac cggcatattg cctggtttga ttattgtcgc agttatttta
     8341 tatcgctttc ccatactggc tctcatctca acaacaaaat caccggctga gccatcactc
     8401 cggtaatgca tgcgaaaaaa agtgagcgtc tcagcaatgg ctcatgcggt gattctgtac
     8461 ggtacccgca caaaatacgg gcaccaacag cgtcgcagaa tacaacgaag gcatcagggc
     8521 agcacctctc tcccgctatt tttcagagtt ctttaccctc ctcctgacac gggagtacaa
     8581 ccctgacagc atccgggcca gtacattcgc aagcacccat gaaaacacag ccacacccac
     8641 agctcccgcc agaaacagca caggttcact ggcaatttga tctgacagcg atggatacag
     8701 tggtcccgga aaatcgatat cgctcctcat atcacccctc tcccagccac aacagcagat
     8761 agaacaaatc cgccgcatca gtcactgata tataccagcg gggatgaata tactcctcct
     8821 ccgacggcca gctctcatgc ccactcacag taagcagaat atgcacatac tgcccctgct
     8881 gaccttccag catcaccgcc acactgctgt tcagacccac cctctgtggc tggcaacgga
     8941 tacactccac ccgccccagt tgcgccgcca gatgcccgcg aagacgttca agattatccc
     9001 gtgtggtgca acttatcccc ggcgcaccct gtggcggcag aacaaacaca cttccggcag
     9061 ggtgaacagc agacaccact tccggttccg gatacttcag acacagcata gtctcactcc
     9121 ctgccaacca gaccacgtag cccgtccagc tttcgtgcca catgaaacgc tgcttccagt
     9181 tcgctgcact ggtgcacctt catcagggcg cgtcgttcag ccttctcttc tttctccgtc
     9241 atccccagag ccagatacag actcggtggc acaacccgga acaacgcttc tgtgcgcttc
     9301 gccagtacca ccccctccgt atagcagccg gacagtttac tggctgacag cagtaccgcc
     9361 ttctgctcct ccgtcaggga tttaaaacgg gcaatattgt tcacttcctc cggcggcatg
     9421 gtcaggcaca cccaccactc tgccatattc agcatcttct ccgagacatc cggataatcc
     9481 ttcaggttct gcgtggccag ccacagccag gcccccagct tgcgccacat tttcaccact
     9541 ttggtcatat acggggacaa aagcgggttt accgtcacga tatgcgcctc atccaccaca
     9601 aacagaatat cccgtcccag atactgatcg cgctcggcaa tgttgttaat catattggtc
     9661 attgacacca tggtcagtgc catctgggct tcataacctt cacgggcaag atgccccaga
     9721 tcaatcaggg tcacatccgc ttccggccac agttttcctt cccggttaaa cagctccgct
     9781 tcaaaactgc ccggctgggt gaacatcccc agcgactctg ccatctcagc agctttagcc
     9841 cggcgaacat cattacgctg agtgtcccgc gaaatttccc acaacgcact ctgcagatcc
     9901 accggcagca tctgacgccc ctcccggtac gtggtgtgcg tggccatcag cagtgcctca
     9961 cggatcatcg ccctgtccgc acgtttgagc gctgcttctt ctttcggatc ccctccggtg
    10021 atcatcatgc gggcagagat ttccatctcc cccagaatgt cgcgtttctc ttcctcttca
    10081 tccccctcat cttcatcaag atcgggcagt gaatgctcat ccacactctg cagcgtctct
    10141 ccctgctcca ccagttgatg agcatccgca aatggaggaa gacacacccc cgttcccggt
    10201 ttgacactga ttttattaac cgaaagcccg agactctcaa aataatccgc cagcagacca
    10261 aacgagttcc cggcctcagc gataaacaga cggggacgat gaactgccat tatctgcgac
    10321 aacgaaccac acagcgtggc cgatttaccg gctccggtag gcccgaaaag cagcagatgt
    10381 gcattctggg tccggtccag cttgttcagc ggatcaaagg tcagcacatc cccgccacgg
    10441 ttaaagaaac tgaatccggg atgccccgta ccggtttccc ttcccgtcac cggcaggagc
    10501 cctgccagat gctgtaccca catcagacgc gtgtaccact gttttttatc cgtatcagga
    10561 tcaaaacaca tgggcagcgc gcgcagccag ctgttcagcg gtccgacatc aaactccggg
    10621 cgcactggct gtaagcccgc gcccagcagc accgtcgtca gttcaacccg tctgcgcttc
    10681 agactctcca tatcctctgc ccgcagcaga aacgtgatcc cggcacggta gagcttgtgc
    10741 cggtttccca gatatttctt cacggtcgcc acatcctccc gggcacgcag ggattcactg
    10801 ttctccccga ccgcattttt cgacaacctc gtgaagcgct cttccagtgt gtcctgtggc
    10861 tggaccacaa ttgtcataca cagcaccgtt ccctccggaa aggtgtccat cagggcattc
    10921 actgtctttt cgccccgggc ctgttcccct gtgatggttc ccggctccgg tggtgtgcgc
    10981 agtttctcca ccacaacagc ggcatgcgga agcccgtcca gccaccacac gccgttttcc
    11041 ggatcagata ccggtggggt gaaccagagg ctttctgcaa aatcggtcat caccggcatc
    11101 attccctccg gtgtctcctg cggctctgca cgggatgcca tacggtacag aatatccctg
    11161 tccacccatt ccggccgggg attgaacagc ctcagcagcc agccatgcac ctgctgcccg
    11221 ttcatccgtg tacagcggac gcctgcgcca cccagcgccc ccacgacccg cgaacagacc
    11281 tggttcagca ttgccaccgg cggcatcgga tcgtggttgt ttttcccgat ccaccggtaa
    11341 atcaccatcc tcgtgcgccg ctgctgacca cgccatggct gacctgtgac cagcgtatcc
    11401 ctgaacagac cttccggtcg ggcaatcccc ttcagatggc gctccatctc cctcagccag
    11461 gcctccgtaa atgaggagcc ttctgcatga ggtttcacgt accccctgag ctggtcaaca
    11521 tacgtgtcca cgtcgttttc atcctgacag aagaactgca ccacccaggg atgtgagtca
    11581 tattcatcaa aactgtcctg cagcgcatcc tccaccgtat cccggatttg ctccagacgc
    11641 tctgcggtcc gcccttccgt agcggccgga ctcacctcat acacggcacc aaccgacacc
    11701 ccatcatcca gcagcaggca ctgctcttcg tccagatatt ccacccatgg cagaaaatca
    11761 atcaccgacg gacaggcatg gtaaaccttc gtctcatcct gtcgggtcat cttccccggc
    11821 cttgacagtg gctttcgtac ctgaggggcc actctttctt tcatatccgg agataccgct
    11881 tctccgtcat gctgcgacac cggggaagaa acccgttttt ttgtgtctgt aaacagggaa
    11941 aatcgcatca caacgcctcc gttcgttcac ccggcatggc atactgcgtc cggctgtaaa
    12001 aaggaaatac tgtgctgtat cccggaaccg gtgacgtacc ggaagccaga tgcggaaaaa
    12061 catacatcac catgtcagga ttgggcagcc gcggaaactg ctgactgatc tcactttcct
    12121 gtgtccggct gtagcttatg gctccgtgcg catctgcctg ctgctccggc attgtcagcg
    12181 gacgccgcag ggtgtccctg gccgctgtca tgtgccgcga atgtcctccg cttcctttcc
    12241 cctgccagat ctccagcata ctctgttcac ccggaggcag aagctcctct ttcgatgtcg
    12301 agcagccgga cagaataaat accgccacca tgacggtcat tacagataac tgtcgtttca
    12361 cgggatctcc ttacatcagt ccagaccacc ggtaccgtgg acttcccccg gcagagtgaa
    12421 gtcgtaacgt acccggcgcc cctgatcttc ataatcaatg gccagttgac gcgtgatatg
    12481 caccgccagc gtctgccccg ggggaacata aatggcatca aacgtctgcc cgtatcgctg
    12541 tttcacccat tccgcggttt cattcatccc gccggacagg gcttttccaa gtgccgcctc
    12601 tccggcactg ccggtcagtg ttgatgtgat gccattgacg ttattctgtg aggtgtactg
    12661 cccctcactc agtgcatcac ccgcagcgcc ggctgcagac agggcaaata ttgtcggcag
    12721 atacgtggag gcattggatt tacgggttcc gctgatacac gggatcccgt catcatccga
    12781 aatccagcca atgcctcctg tactgctgtt attttttgcc tgtgcgttac tgctgccgtt
    12841 cacgtctgat gacggcagcg tacgcactgt cccgtcggca aaaacaaacg tgatgctgtt
    12901 cacctgccca cggacacagg agagcgtcca gtcaccactc gcagtaccgg acaccactgc
    12961 accttccaca tccggcagct ctattccgtt tgccgtcagg ttatccttac ccaccaggat
    13021 cttgaacgga tagggatccg tcacggttcc gttcaccgga acccgtccca gaagcgctgt
    13081 catcgcccgg ctgcctgcca gcgtcgagtt ctccggcagg gtatacactg cccgggtgct
    13141 cgccttctga tgttcattgc tgtatccctt cacctgctgt tcataagccg ctttctgacg
    13201 ggtcagtgga ttgtcatcca gaaaagagac tggaaaacgc ggtccgctct tatcccccac
    13261 cgacgacgct tcatcctgca cctgatcggc cggtgtcacc cacatcagac catccgttcc
    13321 cggtgaggct gccgatcctg tcacgctccc cagtccgtca aggcccagac ctgctggaat
    13381 atcgctctcc tgtcctccgg cgccggcgtc agcactctcc tgctgacgct tcagttgctc
    13441 tgtcagtgac tgaacctgtg tcattaacgc gttctgttca ttccggaact ggcgctgcga
    13501 ctgctcataa tttttctgca atcctgccac tgcctcactg acctgaccgg aaacatccgt
    13561 gccccgcttg cgcagcattt cattttcttt cagcaacttt tcattctgtt tgtccagtac
    13621 cagttgccgc tccctcacct gattcatacg cccaatcagt gtgcgcaggg tatcttcagg
    13681 tgtgtctcct tccacgccca gggccttcag ttcatccggt gacagtccgg ccagcgttcc
    13741 tgagctttcc gcagacgagg gctccccggt atctcctcca cagcttttta atcccgtcag
    13801 gatccccgcc accaccacac agggcaccac atacttcacg atcgtattgg atttaatctg
    13861 catgcttcac ctccccccgt gaaggaatgc gacgggaggc aggctccgcg ataaacgcct
    13921 gttccggccg ttctgccgtc accacataga gcgtggtggt atcctcaggg gtacctgctg
    13981 cacccagcca gcggtgctgg aaagtcgcgg caatgaattt tccctgaagt gcccgcggat
    14041 caaggataat tttgcggctt accgtactcg tcagtttcag tgcaacaact gcacgcccac
    14101 ccgcagccca ggctgccagt ggcgtgactg tcagtggctc tgacggatag agtgtggtga
    14161 ttttcccggg cagatgcgca ttaaccggcc ggatacccgg taccgcttcc agcgtccgta
    14221 acggtgcgta cagcgactgt gccgcatagc gggtcaacag aacgggtaaa ggctctgcat
    14281 acttcaccgg ctgacggagg gcctgcgagc cgtcatcccc gccgggtacc acctttttgt
    14341 gttctgcaga gcgtgcagac gccactgtgc cagcctgact gactgtactg acgcttccct
    14401 catacacaat ccgtacgggc tctgtgggcc ctttttctgc tgctgtcaca tccagcagaa
    14461 taatttctcc gctctccaca tcctgcagtt gcagacgcgt ctgcggaaaa acactctctg
    14521 ctttcagata gactgcccca ccggtactct gtaccctcaa ctttccgttc agtgctggcg
    14581 ggaagcccac ccgcacattt ttatctgcaa aaataacccg ctcctgtcct gccatcaggg
    14641 taacgggtaa gggaatgcgc tcccatttca tcagctcatc agcccgtgcg ctcagtggta
    14701 aaaccagcag tgacaggacc atcacaatac tacggtataa acaacgttca gctccgttca
    14761 tcactgatta tcccccttcc cggctgccgg ggaggctgct tccagccgct gtggtgtact
    14821 gctgtagcag tccagcgcca gcccgaacgg attacgttca ggatccccct ccatgcgcac
    14881 cacctttatc ggataacgga caagtgcccg ttttaccggc tcagcatgaa aatactcatc
    14941 tgccaccaga tccagctgta cctgccagtt atcccgccct tccaccgtca ccggattttt
    15001 gttgtacctg cccccgtagc catgttccgg aatttcatac accacgcgca cacgctccag
    15061 cagctccccc gcatcactgc gacggcgggc atcactctcc agaatctccc gacaggatgg
    15121 cgtcaggtag gcggagagcg catgaatttt cgccgggtag tccacctctc cgttcttcgg
    15181 ccaggcattc agttgctgaa agatataaaa ggcaaaggaa taaaccgttg acggcgggac
    15241 ttcccaccag ggacgggtac tgccggaacg cagatccggc ggattgtgaa ttgtcagtct
    15301 gtccggtgcc gtcatccacc cgatacagac cagcatcaga attccggcca gcacaccgct
    15361 gaaaatacgc agtgtcagga tatgctggtc gcgatcccgc agagcatgac gaaaacgact
    15421 cattttttcc ccctgctacg ggtacggcgc agggaccagc catgcgcctc ccggataaat
    15481 gttttccggc caccacccca tgcactgctt ttcttcgcca gatgctgcca gagccagttg
    15541 tcaggttttc cccgcttcag ccggctgatc cagacgccac cgaaccagac cagcagcagg
    15601 ggagtcagca acatccctgt cgggataatc acccagccaa gtagaggaat cagcggtaac
    15661 ggccagatca cccctgccac agcgcctgtc agggccgcca gccccagttc cggtgtggta
    15721 aaaccacgaa aaaccgcagg ctccacgttc agacgatcgg gcagaaaacg aatggtctgc
    15781 atggggatgt ctccttacag aatgatggag gccgacttgc ccagcagcca gataatggcg
    15841 accagcagaa caacaccaac caccacgatg gcaccaaact gcccccatcc ggccttcccg
    15901 tcacgaacct ctgagaaggt ggaaagcgcc gcttccgcca ccttcacaaa agcaactgca
    15961 ctgagcacaa gaccaaacag cacaacacca tcctggagat agcctttgat ctgtccgaaa
    16021 agtccaccac cgccaccgga tgttggcggc tctatctgag gcaaatcagc cagagcaggt
    16081 gtgatcccgg tacacagaaa caccgccatg gcataccagg cctggcgaca gatgcgcatt
    16141 tttttatgca ttattttctc ctgaaactta aacaagacac tgaaatcaac ccgcaaacat
    16201 ccagatactg cagatcagca gcaggacgag tcggaacata aaccagccga acacaccatc
    16261 ccggacatca ccgctacgcc agcctgccca ggcatccgtc agactccacg ctgcccagag
    16321 gaacacgact gccagcagaa atcccagcgt gatgagatgc agcacactga gatccacttc
    16381 acctgaagca gcccggaagg ccgtattctg cagggccgtc atcgccatca gggacgctcc
    16441 cgccggtagt caccggtcac ggctgcggaa gaccggggtt gcgcccggga aggggccaga
    16501 taatggtcaa taccgtcacg aatggtctgc agatctgcac gggcacgcag ataatcaaaa
    16561 aagtagcgtt ccccctgtga agaagcctgc agagaggccg ttctggcccg ctccagcgct
    16621 gcctgcacgt ggtcaagctg gcgcattaca gtggccagtt catccttttc ggcagcctgc
    16681 gcaggtaatg caaaggacaa aagaaacagc atccccgcgg caccggccag acggccggca
    16741 cggcaggatg atgccggaaa agaaaacata cgcacctccg tcagagttaa acagtgcgta
    16801 caggatgcgt aaggcagggg actggatcag cgataaactc tatttgcaaa acagaaaatt
    16861 tttacgggag aatacactgg tgtggatgaa ggcaaacaac ccggaactcc tggcatgaac
    16921 gacatcatca gtataatcat tctcctgaca gtctatctca tcggcctgat attcattatc
    16981 ctgactatca atgatataga cagatcatca aaaaagagaa agtacgagaa aaaactgaaa
    17041 gaagcgataa taaatggcag tttacaaaat aatgatatct gtttactggc cgaacgctgg
    17101 tctgccagtc atgaacatat caaaagcgca ctgctgtctg ttttcaatga ttttctgact
    17161 gataacaact tttcacatca cgtcccccgg gtcagagaac tgatcacatg gtacgagaaa
    17221 tatgccccat tcacagaact ccctgaggat ataaaacaac agttacaaaa tataatcagt
    17281 ttacccgatg tcagtgagtc agaagtgcag ttattagcca cgtcacttca tgacatttat
    17341 aattcctgtc agaaaaaaga gaagctggcc agaagaataa actatgttct gggggctgtc
    17401 agcatagcca gtttctgcat ctcgctgttc cagattctgt tcaaataaat ttattccagc
    17461 ctgaatgatt tattcctgtt tattaaaaca cggcatcact actgtttccc gttaatggca
    17521 ccgcattaac aatttaaaga ggaaacacat cagcatggaa tttctccgta ctgcccttcc
    17581 gttctgggcc ctgttccttc tgacctgggt ttgtgttgtg tattttttca tttatgaaga
    17641 acctcgttgc cccccgtgga tctatgacgt catcagggcc atcactctgt tcattatttt
    17701 cgtcaacatt gttgtcattc cggcgctgtc gttactctga cactgacgcg cactgaccgt
    17761 aagggcgtgg cttgtctgcc gacaccctgc cacaaaacaa gttttgcatc agggtgtccg
    17821 gcctctgtgg attataaata tttcttgaag gatgccatgg tcacggtgat ggccaccccc
    17881 agcagcatgg ctgccggcca tatcagcagg ttcggataca ccgacgtcgg ccaggacagg
    17941 taaagaatgc agggaatata tatcgccggt ttaatagact tttttgcatg gtgataaaca
    18001 aagctggact catacgcagc accataccgg cgcagatcac gacgccccag tccctccaca
    18061 accgcgatca gtatcaccat cacaaacagc ggaacagaca acaccaggat cgtcacccgt
    18121 atcagtgtga ccaccgtgac atatactgtg gccatcaggt actcatacag ataatggctc
    18181 agccagcgcc ccacatcctc actgtactgt gacacactgt tacccaccac ggcaccggca
    18241 ttttgctgct tcagccacga cagaaaacct gtttccacaa acagccactg atacgccgtt
    18301 gctatccatt ctgtcaccgt taccgatggt gccgacagca gcagactgcg ggtgaattcc
    18361 gaagacagcc accccagttc tgtcttcatc actgcctcac tgtgtgctgc cccctgctcc
    18421 ggccagaaaa aagcaatccc gatatactca accagcaggc tcagcagcag ggaggaaaac
    18481 aggatcccga ccagcatcca cggccagccc cacaacagat tccagagcag tccgtgctga
    18541 cgtggcggag agggttgtgc tgctctgttc acctcacgac ggggttcagc cattctgctc
    18601 tccttccggc atgttcacgg agggagtctc attccaccat ccctctccgg tgtggtaatt
    18661 acgccgcatg gcctctgcaa tttttgcaat actctccggc atcagcgcgt catctgcatc
    18721 agttgcaggc aggggcatac ggattttcca caactgtccc ccttccagca gagcaaaggc
    18781 ctgtcctttc ggcagggtca cgatatctgc cggctccagc aaaggcactt tcacagtacc
    18841 aacacgatcc tgggtactgg atgtgaagtc ctggccggtc gtgacatctg cggtgtcctg
    18901 atggccggaa accagcgttt tggtataaat ttccacctgg gacaactggg tcgtcagtaa
    18961 ctcggccgtc cggttctccc tgacccgcag cataatcatg ttattaaagt taccctgcac
    19021 ctgggccgtt ttggcagcac taccggtacg ggcctcaata tcagaagagg tctgcgtgta
    19081 agccgtcacc tgcataccgg caccgcctcc cttgttgatt aacggaatga actcatcgcc
    19141 catcagttca ttaaactcat cacaatgcag gttaaccgga tttttggtac cattccctcc
    19201 cggaagaccg gcattcattc cgtgtttgta gatatgcccg gcgacactca ccagatccgc
    19261 aaacatggag ttacccacgg cactggccac ctcactgtca ctcagcgcat caagaccaat
    19321 gtacaccacc gcctttttac gtatcacctg ctcccagtca aacacgggac gggtatcgct
    19381 catatccagg tagtcaggtg ccagcagttc tgccgtcttt cctgtggtca gtttttccag
    19441 caacggcaac agggatgcca caattttgtc aaaataggta cgatcataac gtaccgcgct
    19501 tcgcagacca tccagtatcg gatcatagag tttctttccg gcttcactgc tgagagccac
    19561 ctcaatggcc cagatacgca cagcatccgg ctggttctgc atggtccggg gaacatccgc
    19621 ttcccccagt acattcatgt tattcagaat ggcgttttcc agttccggca tctgttcgct
    19681 gatcacctta ccagcataac ggatataaag gtcggcaata ttattcacat aacgcataat
    19741 cagcgtgtaa tccgggcgct cccccagtgc caccagtgca cgggcaacaa tgttgacaaa
    19801 ccgccatgca aactcacgga aggccgcact gttaccttca ccggataact gaccggccac
    19861 acgggaagcc acctccgaca cacgaccaaa acgccccacg gcgttataac gcgcagagat
    19921 ttccggccag cccagatgaa agatataaag ctcatcgccc ctgccggcac ggtgtgcttc
    19981 tgcccagaca cgcagcaaca ggtccgcatc ccctttggga tcaaagacaa tggtgacatc
    20041 tccccgacga atatcctgtg tgaccagcaa ctccgccagc cgggttttcc cgacacgggt
    20101 ggtgccatag acaatggtgt gccccacacg ttcacccagt gccagcgtca catcctgctc
    20161 atccggctca ataccatgca gggcaggatt tcctcccacc ggcggcaggg gacggacggg
    20221 attcagcggc gagtcacgac gaagaagctg cccaatccag ggaagactgt gctccgtttt
    20281 catttcaagc cagcgcgctg tccggtacag actgcccggc tgcagatagt gtgcaacctc
    20341 cggtcgcagc gtgtcctgaa ggcgctgggt gtgcttctgt gtccagcgga agccgcgtcc
    20401 cagaaacagc cgctgacggc tgaccggaat ttgccgggaa ctcatcacat aacggggcag
    20461 ccgacgcaga ttgcgacggt aacggatgat cttcatcccc tgtcggaaac ggaccactgc
    20521 cagaacgcca aagccggcgg ctgtgacata gctgaccgaa ggcgcgagcg ccaccgccca
    20581 gggcgctgtc acacagacac cggccgccgt tgctgccacc acggcggtat tcagttccac
    20641 agccggacgc agcagagcct caatcacata acggttactc atgaccgggt acctctctgt
    20701 aaaagacgcc gccaccatga gcgcggcaac gctttctccg gtgacgctga cagtgcctcc
    20761 agcctgcgct gtaacaccat cacctcccgc catgcgtctg ccgcattcag cagtaatgcc
    20821 ttatacacca gtggcccgtc atgtccgccg gcatgcaaat catccactgt caactgcgcc
    20881 tctgttctga aacggtccac tttccagcag gccaggtcct cgcgggaaac acactgccgc
    20941 cggatctgca gacgaagata cacatcatgc cgggacaacg gttctgccag gttgtgaatt
    21001 tcccgacagg cagaccggaa cgcctggtct gtgtgataaa tactctgggc cacattgtga
    21061 agctgtgtgc gcccctcccc ctgcaggtgg aacagaaagc gggccagaca gctgcggtga
    21121 ttaccgtcag cactgacata ccagtgaatg ccatccgtgc tgacaaaacc catcccggca
    21181 tgctgttgct ctgcctgcgt gtaatatccc ggattctttt cgagtaacgg gatattgatg
    21241 tccatgcgtt taccacgctc cagcagctcc agccagctca ttccggcata ctgtggatgg
    21301 tcagtcccca caatgcggta cacattgata ctgccctgtc ccgtccagta gtgttccgta
    21361 ataaaatccg acattgccgc aaactctgcg gtatcccatg caaccagttg ttcatgtgcc
    21421 caggccggag cattcgcctg tatccgctct gtaaatgccc ccttcattca gctcttctct
    21481 cctgcacttt tgagataccg ataccgtcag gccacggcat aacagccggg ctccccctgc
    21541 cttccccgga tacggctgca gaaccacaac cacgaaacag aatgactgac gagacacctt
    21601 ccataaacaa agcgcccaga aacagaacca ccgccagcat tttcagttca cgaatatctt
    21661 cagcaacagc ctccccgaaa gaaatcgctg ttcgcggaca ttcctgctca tcgacataca
    21721 tccctgagac ggatatgacg gaggaacatt tttgtaccat cacatgccct tcaggcgcat
    21781 cacgccagcg ggatgactcc gtcgcataac gctgtgtcat gcctgcctca ccaccattca
    21841 gattgttcca gacatacagc attaccagac atcctgccag cattgacggc cagacaatca
    21901 gaagccggag cagcagcata acaaaccggt acagtgtcat aatcatgatt ctcctttgtt
    21961 actcagtaag gtgccattca gcagttgcaa cagacgttgt ccactgatca gctcaatatc
    22021 cggccacagt gtcagccgtg cccggctgat gttccccgta cgccccgtat ggataaaaaa
    22081 gcccctgcat cccttccggc gcagcagttc gccaaaagca tcaatatgtg ccggacgaat
    22141 ggttcttctg taacgtttag cctgtatcag ccagcgttca ccgttaatca ccacctgacc
    22201 atcaagacca ccatcgccgc tgtatgaggg gttacggata accggcatcc cctgttgctc
    22261 aaaagccagc agcaacagtt cctcaaacac atacggactg attttgcgca gatatttcag
    22321 ccgccgggca tcgccctgca attcgttcag ccgcttcatt acccgtgagg ccgtggcacg
    22381 ataacgctga tgccggcgtg ttctcacaca cgcttttttt cgccggctgc gccacagtgc
    22441 cggccccagg acaacagcca gcaggctgag aaccaggcag gctgtcagta ccggagaagc
    22501 agagagcgca gcagaagtgg cagacagcgg catcatggtg caggtacctg tgacaggccg
    22561 ttgtccgtta tcagcaccgg gtaatgactt atctgtaacc tgcgggccag tacagaaccg
    22621 gatgccggaa ccatcaccac ccccggtgcc agctcacgca gtacgcgcag actgctctca
    22681 tccgccacat tcactaccat tcccgccgcc ttccgctccc tgagtactgc tgcattcatc
    22741 tgcagccatg tgcgggaatg ggcatcatcg cccacaagaa aaagtgcacc gatgcccggt
    22801 aactgaagcc ggcgcgccac aacctctccg ggtgacattt caggcgtact gaccggtaac
    22861 attgccgcct ctccctgcgg aaaagagggt gatggcacaa ctgaagcggg aaccggtggc
    22921 tgccggttta tcgcctcaaa aaaggggctg gcatccctgc cgcccaggtc tgcaatcact
    22981 ttcagttctg cgccagctgt tgtacagaac agcagcagac tcagtacata aaacagtctt
    23041 tctcgtttca tttcagctcc tcctgtggtt cagtccagat ataacggggg gatggtgtca
    23101 gcatggcggt cgctgccaca tgcacatcag acggattcag gaaattatcc cggtggagcc
    23161 gggcaagatg ccgtctgacc acggcggtat aacgtgcggc cggcgcacca cctgccgggt
    23221 ggtgataaca cccggccacc tgcagccagc tgaccggatg gcgacgccgg cattcctgca
    23281 gaatgaccgc tgccgtattc agactggtgt aaggatcaaa tgccgcccat gtggacggga
    23341 aacgatgtcc attccagccc agattgacct gtgctatccc gacatcaata cgttttgccg
    23401 gataatgttt cagaaacacc tgcaatgcct gccaggcctc cagacgtgtg gcataacggt
    23461 acccttttcc tgccacattt attgtccagg gccaggggcg ggccacgtcc ggctgacgaa
    23521 aatcacgacg ccgttgtttc agtactccgg acatcgcttc cggagacatg gacgtttccg
    23581 tcagggaaac ggcatacaaa agctctgcgg gaataccatg ggcacgggcc acccgcacgt
    23641 acccgtccgg tacgttctga acggcagcac gacagacacc actgcaggcc accatcagca
    23701 gaaccgtcag aatgccgcca cgcgccatcc gtcagccccc tgctgcagta ccaccggcat
    23761 ttttccttca ccaaaacgca gccagcgtcc ggcatcatga ttcagggtga tatgacggct
    23821 gcgaactttc tccacaggta tgctgtgcgc atgcgcccac tgacgcagaa tctcatcctt
    23881 tccctgactg tcgacaaggt agatatcaac ctgccgtccc gtggccagta cggaggcaag
    23941 acgggcatca caggtgccac attccttcgc cttcacaaac agcgccagcc gtccccccgt
    24001 atctccggta agtgctcctg tttttcccat actgacaggc aacataccgg gatacaggcg
    24061 cttccaggcc gcattcacct cctgctgaaa cgccagctcc ttctctgttc gttcatattc
    24121 agccctgacc cacttttcgg cccagacacg tcgctctgcc ggcgttctgg cctctatacc
    24181 gagtaccgtc agcggatcaa gtccgggaga ctgtatcccc cgggggccgg acatcagtga
    24241 ctgataacgc cggtaatcct cctcactcac cccccactgc ccggcctgct gctgaagtcc
    24301 ggatgtggta ctctgcgtca ccaccgtacc ggtcgtccgt tgcgcctctg acgacatatt
    24361 cgtctgcccg gcagactgag ccagaacggg gcacagtgcc agcagataca gtatttttcg
    24421 cttcatcatc tactccaccc tgagcaccgt ctgacgacca ttcacccgga acgttgcttc
    24481 accatggccg gcatacacaa gcgtccaccc ggccacactc tctccttcac ccagcagagc
    24541 cacctgagag aggctggtat acccccgggg agccacggct gcacgggtac tgccaccgcg
    24601 cagctctgtc ccggtcagca caaaaggagc ctgccgaact gtatgaactg cgcgggattt
    24661 acgggaggac gtcaccggag cgggcttttt ctcctccgtt tttttcatcc ccgctttcct
    24721 gctgtccggc tgtgacggtg actccggtac tttctgcatt ttctccacca gaccgctgag
    24781 tgtatccagt ctcagggaaa gctttttccg ggcagactca atctgctcca gcatctccgg
    24841 cagattctcc agttgttcct tccaggatgc tgtcagcacg ggctgctgct ccaggactgc
    24901 caggcgcttt tccatatcct tcagttgctt ctccgtctct gtcagtttca ttctcagggg
    24961 cgcgagttct gacgcagaaa gcatttctct tctctggcct tccagggtcg tgatccggct
    25021 gtcaagaccg gtcataagca tcagtacgcc atataccact gcccccatga ccagccccac
    25081 agtgacagac agtatcaaca cgccggggaa acgacttttt cttcgctccg gcgacatacc
    25141 agacgcaccc tccggtatct gcggctgttt ctcactcatt ttttcagaaa ccctccggtt
    25201 gccttcacac ccggttcacg acccacaaaa ggaacggtga ccggaacagg agccagctgg
    25261 ctgaccggta actggtaacc gtcacgcaga ctgtggcaga ccaccctctg cacatcatcc
    25321 acttccagtt gccatgccgg ccccgccatt aactgcaggg cagtacgcag tctcagtggt
    25381 cctggctgat actgcaccgc cggcagaggc tgacggtaca gcacgccgtt ggccggtccg
    25441 gtggcgcaga gcgtgtaacc ggactgtttc agggcataac gcatggcatc ggccaccgtg
    25501 ggtttcaggg aggagggaat gcgaatgtcg atcacctgag aaagtggatc acgctgtaca
    25561 gcctgcggct ctgtgctgac cagcaggtaa cggtcatagc gcaccacctc cggtgcctgt
    25621 gaatacacat ccgggctggc aggctgtaca ttacggttta ctgtgaccga cggtaccggc
    25681 atggccgcat cccgttgctg tagtttctga gtgccctgac atcccgccat caggaggcag
    25741 actgtcagtc cggacaggat caagcgtttt ttcatcgggt atttacctct gcagtgataa
    25801 atcagaggtc aatgtgcaga agaagacggg gctggctcaa tgaacaactc attacccgct
    25861 ggcgaaaaaa aacgcctgca gacatgcagg cgctttgttg acaggaagga aagcaacata
    25921 acaaatatgt cacaatcctg cggttaacat aaattaccga ttaccggaga gcattacgca
    25981 aatcatcatc cgagaactgc agtatttgtt tgattttctc tgacgatata cccatctgct
    26041 gcatgcgacg ggccgatgcg taattctcct gaagagcctt ttctctctcc tcctggcggc
    26101 ccttctcgat accagccagt tccagtcgtt cagcgatagt cattagatca tcctcatgtc
    26161 tgggcagacg ttctgccatc gcccgtataa attcaggtga agctgaatca cattctttca
    26221 ccatatagtt aaacaatgtc accacctgag tgtgtgtttc ctgtatctga atcaactgtg
    26281 acaaaaagtc aagatgaacc atcagatcca ctttcttctg ccggagcttc atcataagca
    26341 tcagcgcagc catctgctta tagcgacgga taccctcatc atcaagaaga ccgacatcaa
    26401 tcaccctggg tggcctgcct tctccataaa gctcccgacc aaacgccgga tcatccaggc
    26461 agtcaggcca gttcatgctg tatggataag gactgggtac accatggtaa aaaagcaccg
    26521 gtatcacaac gggagcacac gcatgtgctt tcaggtggcg atgtatcacc gccagaacat
    26581 agcgcatcat tcgcagggcc atatacttat ccgccttgct ctggtgctct gtcagcgtat
    26641 aaatgtatcc atccctgcca tctgtggttt tcatactcca cagaacatca caggcaaact
    26701 ggcgaagatc cggatccaca aacgtggtcg gctccagttg cagggtgttc atatcgcacc
    26761 ggttcagttg ttccgggggc aacgcgcctt tcaggaaatc cctcgcaaca gagggacatt
    26821 ccatcatcgc tctgaacgcc gcatcatgtg gcgtgggggt agtgggtgtg ttttcattca
    26881 caaccgcctc cttgaattaa aagtggggcg gtaagccccg tggttaacat cagatgacat
    26941 ggataatccg tcgttcaacg ccatacccac gccccataaa ggggatatca tcgtcaaagt
    27001 ccatcggcgg cattcccccg cttcctgagg aagcctgacc gttttttccc gttgattgtt
    27061 gtgcattttg tggtggttgt ccccatcctc cctgagcagg agcagatgaa acatttccac
    27121 ccccctgctg ctgacgcgga gaccccagca tctgcagggt accattgaca ctcacaacaa
    27181 cttccgtgga ataacgatca acaccattct gctgatcgaa ccacttccgg gttcttaact
    27241 gaccttcaat atacacctgt gaacctttac gaaggtattc accggcaatc tcagccagtt
    27301 ttccgtaaat cacaacacgg tgccattcag tgtgttcctt catttcaccg ctctgtttat
    27361 cacgccaggt atcagtggtg gccagcgtca gatttgccac tgcacctccg ccgttgggga
    27421 tataacggat ttccggatcc tgtcccagat gaccaatcag tatgacttta ttcactccac
    27481 gcgatgccat aacaactcct tatcctcaca cacattcacg ctgagtccag atgccgtttt
    27541 ccagagcccg gactgcctgc tcataatttc tgaaatactc ttcggattcc cgggacacag
    27601 gcccttcgtc gcactgagta ccaatataat aaccacgagc actgctgagg atctgcagag
    27661 gtaaggtata gcccgctttg gcggccagaa ttccgcttgt cggtttcata acatgtttcc
    27721 ttaaagttaa acgataagaa aacagacctc cctgatggtt ggtctgtttt catcagggag
    27781 gttgagttga aaaatacgat cagaaagaat tttctgcgta ttcccgcggg gtattgccat
    27841 tctgtggcgg tatggattcc gatttttcag tcttataaac catgtcctgg cctattttga
    27901 tccactccac cttgatcagg cgggctttca gactgacgcg ttgctcaccg gcatgttcac
    27961 cccggttaag ggtaaaaatg tcagtagagg ggttgctcat ggtaaaacca atcagcacct
    28021 tgcgatcttc atccaccgct ttctggcagc gggcaatcag ggaagttgcc tctttaccgg
    28081 caaccgtcac atcaaaacgc acataggacg ggctgtccgt cggaccactc agtgcattca
    28141 caacacaact gagaaatgtc ccgttctgat gattaacctg acgaatgttg ctgagatacc
    28201 ccaggccttt cagtgtcaga ttgaagtact cagttttagc gtttgcagaa gaagtatttg
    28261 cagtcataat gatatctcca ttctcagtat cggaaagaca gagaacatca tcatccctga
    28321 cgggttgtga tattcccgtc agggagccgg aacagaacta ttccggctga ctcagtaaca
    28381 gttaaagaaa accttcatcg tcatgactga cgatatctgc tctcaaaggt gggcagattt
    28441 accaccacca gaaacatcct ggctgcacat cccctttcag gttacggggg tgttgatcac
    28501 ccgaaggcgt tcctctcaga tcaacggaac attggtgccc accgaagcgg gatgtggtca
    28561 cccgaaggcg ctcctctcag accacttatg gagcattggt ggtcgccgaa gcgacatgta
    28621 gccacccgaa ggcgctcctc tcaggccact tgtggagcat tggtggtcgc cggagcgacg
    28681 ttatgtgccc ggacagactg aaatcactga tggaaaaagt caacagctaa ccaaaaacaa
    28741 aaaagcaccg gcaagccggt gcttcagggg gataccaaat cccggtgttg attctcatct
    28801 tctccggaga gagcgactcc cggcacggtt ttcacggagt gcatgtgcct tgtaaccccg
    28861 ccgtgtgcat aacgggttac cggacctctc agggctgtca gccccactgt ggtcaacagg
    28921 gtgataccag aactcgcccg caattttaat tcagtttgtc tccgacatca ccagattttt
    28981 gtttatgaga aaaattaatt tttcttttcc tgcccctgct gtttccctgc tcaccagcaa
    29041 ttcccctgca gtcgggataa cgggagcatc cccagaaaaa accgcttttt cccttccgtt
    29101 tcaccgtcgg accgttacag agtggacatg aaggacctga ttccactgac agattaaccg
    29161 tctggttacg tccggataac accaggtgcc tgatccactg atgctgtttc tccataaacg
    29221 cctccagtga gattttcccc caggcaacat catccagcgc ctgttcccat aaagccgtca
    29281 tacccggatt tgtcagagag tccggcaatg ccgccaccag ttctcttccc agttgcgtgg
    29341 aacggatatg ttttcccgct ctctcaatat agttgcgcgc aaataacgtt tccaggatcc
    29401 ccgcgcgtgt ggcttccgtt cccagtcccg cattctccct cagaatcttt ttcagcgccg
    29461 gatccgttac accagacgaa gcattcttca tcgcagtaat aagactgcct tcagtaaaag
    29521 gacggggagg tacggttttc agctccttct gttcagcacc atccagagca cagagttctc
    29581 cctttatcaa agggggcaat atgtttccac tttcatcact gtcaggcttt tcttcatctg
    29641 catgattttc cggtggaaat aactgtttcc acccgataat cacattaact cttccgcggg
    29701 aacggaataa ctgcccccca atcacaaatg ttgcctcagt aacatcagct tcctgctccg
    29761 gcagaaactg tgccagatag tgctgacgaa tcagttgata taccttcagt tcatcaccgt
    29821 tcagtgagct gaggtcaaaa acctgccggg tcggaataat ggcatggtga gccgtaattt
    29881 ttttatcatt ccagatacgg gatacccgtc ctgattccag ttcagccagt tgtggaagca
    29941 cggacggatc tgaacgcccg acagcctcaa gcacttcaga actctcctca tgcatcgagg
    30001 caggcagata accacagtct gtcctgggat aagttgttgc tttgtgagtc tcataaaggg
    30061 actgtgcgat atccagaacg gtctgagccc ccatgccaaa cttccgggaa caggcttcct
    30121 gcaacgttcc cagcgaaaaa cacaaaggtg gcggtgtttt ctccctcttc tgtacaacat
    30181 cctgtacaac agcagaggac tgctgacggc acagaccgag aacagcaccg gccgcctgca
    30241 cattcacaca acgtccctca tcatcgcagt actgctcagc tgccacccag ttactgcgga
    30301 aacggagccc ctccttttcc agccagacaa tcacctgcca ccaggaaacc ggcacaaatg
    30361 tggcgatctc ctgatcccgt ctgaccacca ttgccagcgt gggtgtctgc acccggccca
    30421 ctgacaaaac cccagaaatt ccacgttccc gtgccttcag ggtatacaac cgggtcagat
    30481 tcatccctgt cagccagtca gcccgggcac gccccttacc ggcttcactg agaagcgtag
    30541 tctgttcgcc cgggagaata ttattcagcg ccttcctgat actggcctca tccagtgcag
    30601 ataaccacag acgcctgaca cgccctctgt actgacagta ctccagcaac tctctggcta
    30661 tcacttctcc ttcacgatcg gcatctgtgg caataaccac ttcagaagcg cgttttaaca
    30721 gcccccggat cacaataagt tgttttttca ccttcggctt caccaccatc tgccattgtt
    30781 ccggtatcag cggcaacaca tcagaacgcc atggggaacc gaactgttca ccataagctt
    30841 ccggctcagc cagttccagc aggtgtccta tcgcccaggt gacaacaata ttgtcatctc
    30901 cgatgtatcc gtcgccccgc ttactgagcg ccagttcacg ggcgatatct ttcgcctgag
    30961 aaggtttttc acacagaaag agttgcatac tcaccccgct ttaaccggtg gtgaataaac
    31021 agaacgtttc ttaccgctca tcacgtcagg atccggctcc cccatgcggt taaccgcctc
    31081 ctttccacgg gctgtgccgg acaaaatatc agcacgtgta acactgactg atttataatt
    31141 ttgaataatc ccgtaaatcc tgcgtaccgc ataccctccc tgattcagca actcatcccg
    31201 ttttgtgcgg gaaataagtc cgtaatgaaa tacctgaaat accttcatga ccagtcgatc
    31261 atatccgatc agaagccata cacatcgata accgaccgga gaactactgt acacgccaat
    31321 atttaccggc gaagaggatt taatatcaga aagggagatc tgtcccggca ggtttttaag
    31381 gatcccgtca agccacgcaa gatgcttttc aattgtttca gttgccttcg tcagggcatt
    31441 ttccagcctg actaacatca tatctgcata aggattatcc ctggctgaat ctctggttgc
    31501 ctgacccgca aacgcaatag ccctgggcat actgatgatg tcaggccgct tttcctgttt
    31561 ttcttccttc tgattattcc tgcgtccctc ccagagacgg atcgcatatc gggtatgaag
    31621 ggcaatagtc agcgatgact gcagagcacc aacttgttct gattttctga cttctccggt
    31681 tttctcctgg ggcattgtgt ttactccaaa tgatttcatg accagaagat agtccgcagg
    31741 ttgcgtatta ccgacaacaa aaaaacaaat caggcagaat atacatttaa cgccgattat
    31801 tttttaatcg ctgttgccac tggcaacact gctcattttt ttaatctctg ttgccactgg
    31861 caacatcgct cactttttaa tcactgctgc cactggcagc actgttcgct ttttaacctc
    31921 tgttaccact ggcaacaccg ctcacttttt aacctctgtt gccaccggca acaccgctca
    31981 ttttttaatc actgttacca ccggcagcac tgttcgcttt ttaacctctg ctgccactgg
    32041 cagcactgct cattttttaa tctctgctgc cactggcagc actgttcgtt ttttaatctc
    32101 tgctgccact ggcagcactg ttcgcttttt aacctctgct gccactggca gcactgttca
    32161 ctttttgacc tctgctgcca ctggcagcac tgctcatttt ttaatccctg ctgccactga
    32221 cagcactgtt cactttttaa cctcttctgc cactggcagc actgctcatt ttttaatctc
    32281 tgctgccact ggcagcactg ttcacttttt gacctctgct gccactggca gcactgctca
    32341 ttttttaatc cctgctgcca ctggcagcac tgttcacttt ttaacctctt ctgccactgg
    32401 cagcactgtt cactttttga cctctgctgc cactggcagc actgctcatt ttataacctc
    32461 tgttgccact ggcaacaccg ctcatttttt aatcactgtt gccactggca acatggttaa
    32521 tgatgactac taccttaacg ttcagagaca aaaacagcat taatgtcgtt atccccatca
    32581 cgagcaactg aatgtcggtt atatccagat gagacaatga ccagaaaatc acaaaaaatg
    32641 aaaagcattc tgccagcaga ctcaggagga gcataaattg caggccccct ccgactgtac
    32701 tgcctgtacg cgctttataa agactgtatg tgacccacag ccagttactt aacaccagga
    32761 aaaaccaccc tgtggcacat acgatataac acatactgtt atcgtactgt tcgtgcattg
    32821 ttttcagcgc agtgagcagg gtctctgtgc ctgtcaccag cccccagata accaccactc
    32881 cggcaatcac gcaggcacaa attttagcga tcaacaggcc actttctttc atggctgctc
    32941 tcagactttc ctgtttgtat ttctgcatgc tatttaccac cttgtttcac ctgaccagtt
    33001 cccgattctg ttttcttttt aaccagccgc tggcggcact ggttgatttt tgttcccgcg
    33061 aacatagccc gtattgcaat cactcagatc tcatcgcaga acatctgcgc agcgaggccc
    33121 gtagtccagc cacaatgcca ctcagacgtt ctgctgaaat cggttccgta cgtttccccg
    33181 atgaaactga ttgcgcagta gctcgccctc tttctcctgc cagtacggag gctggcacaa
    33241 caacacgttg tttctgcttc ggcggataaa aatttccctc ccgggctcgc ttcagcactg
    33301 caagcaacca gcccacagga ttctgcagcg agtggctgcc cagcatccct gacagacttt
    33361 ccagaacccc ctgcgccagt tcttccggca gcgcctgcaa ctgactggtc agcataatgg
    33421 cctcctcctc cctgatttgc cctgtcagcg cttcaggcaa tacgtacgtg tttttaatca
    33481 cactgtgtgt gatattacgt acgtaatggt tcggtttccg aactctgtga taaccttctg
    33541 atttttctga tggttcggaa tccgaactca gcattttcaa taccgaactc tgtgtttcta
    33601 cccccggttc ggtttccgaa cccggctgca ttctgtcctc agtcacccgc tgacgggccg
    33661 ccatctgtcc cggtgtctgg gcactcccta tccgggcttc cagttgactg atatggctgt
    33721 gataatgacg cattcccgga tcatttttta tttcgcacag aatatcccgg gccgtctgac
    33781 ttatggtccg gttccggctc atacaggcat ctgccaccat atccagataa cgtggatcca
    33841 gtatttcagc atcccggcac gtcaccggtt catcgtgctg ggcataaata ttgccccgta
    33901 cccgcccttt ctcatcacgc acccgtttgc acagactcag ccagcccgtg atccgcagca
    33961 tcagcagcac ccggctgaca gtctccctgg aggcttttcc tttccccggg gaagccagct
    34021 gcagttgcag ttcgtcatat gtggggaaaa ctgcaccttc attattctgt gcatacagcc
    34081 gaatcatcat ccacgccatc ttatccagcg gagaaagtcg cgtatccaga agcagtcgac
    34141 gcggaatggc atcatgcaca tttccggtaa acagcagtcc gctttgtatt tttccgtcct
    34201 gttgtcggcc tttttgctct atccgctctt gcattttttc cagcgtgtac gcaatcaggc
    34261 tgtctacggg taatgtcatt acacttttcc tttttgttat acgtcagaca tccgtcggct
    34321 ggtcacgggt atatcgccca tattcaccag acagttacgc cggagatatg ccagcgcttc
    34381 atcaggaacg gtacattctg gtctggcttt tttcaggtta ctgattaatc gcttcatcag
    34441 cattactggt tcatgtgcac ggatccaccg tccccctgtt tgatcttcca gacagcgaat
    34501 taacatctca gctggcaaaa gaaacccgtg gttctcacac tcctttttct tctctgccgc
    34561 agttaatttc tgttgcaatt caacaattgc tgccagcgca gatttaatcg tttcactcgt
    34621 cacccgacat ggataagcac gattcctgcc ttctttcaga agcactgcat aaccatcaga
    34681 gcggtagtga taacagtttg ctcccaacca gatttcgtaa gtatcgccgc gcgaggagat
    34741 acccgttctc tctgcaatag ccatatctca ttcccctttg atgtgaatga cagtagtgct
    34801 cattctcctg atttcccgga gcacacaagg aacaccacca cttccgaccg gatcgcgtgt
    34861 actcttcagg gcgacacttg attccgccca gctttttcct gaaggaagtt ctttcacacg
    34921 aacaaaacca gctgcccgaa gagatgctcc ggattcatca gccctggtgt atgtaatgca
    34981 gcggcgatat cccatagctt ttgccgccct ccagacagcg ccataaagtg cgctgttagc
    35041 gttacgttca ccagtggtac aggtacgatt gacctccagt gtcagcccat cgtcaaagtg
    35101 gcgggcaaca ggtctgccgg ctgtcgccac tcctgtcaat tgttcagcct cccgcagtac
    35161 ctgaaagtta atttcggcca tgtcatttgt ccttgtcatt ctgctttttc ccggttcagt
    35221 ttccggcgac tggccgatac atcattaaac cccgcagttc cctgatgcca tcccctcacg
    35281 gcatgccata ccgccgtcag cgggatattc atttgctcag ctgccagcat catgacatcc
    35341 agtccgtccg cactctccac atccgtgatg ccgctcttct gccataaacg ccacagctct
    35401 gcactttcct catcactcag tgtgatgcca cgtcccgggc gaacatcaat accggcaatg
    35461 cgacggcgcg ctgccacatc cacactggaa agtccaaagt aaaacgccat cagttcaatg
    35521 gagcctccca gcgccagtgc ccggtcaatg cgctgcagac gtttctgctc cgtccgggcc
    35581 tgctgcagca tacgcagcag gttttcatga ttaatgtcca gccggataac cgacacttca
    35641 gagtttgaca ggtaatgaat ttcctcaatg gtcagtccct gcagcatctg catttcctcc
    35701 cgggccagcc ccagagattc gcagcgacgc aggtagccgc tcttgaggtc catcaccagt
    35761 tgcatcagca gaccgttggt tgcctgagac aagctgttat tcatgacgcc tcccgtatca
    35821 tcagacccgg caccacctgt cgggcactct cccccacacc atgatgcagt gaggcatttt
    35881 tcccggcctc atacccctgc cagcgggcca ggtcaccgcc gcgacaggct ttcatctcac
    35941 gggagtccac cgtcaccata tcctgctgct ccatccagag acgcatcagc cggctttcct
    36001 cttctgtcac actgaatggc atcaccacct gacgaatccc ctgaacccag ccatcacgaa
    36061 actgagccgc ccgggcacga cgggtgctca gtttcagtct ccggctctgt gtacgaagat
    36121 acgcatccgt tgcttccctt agctgacggc acagaacatc aaacgcataa gccgccacct
    36181 gaggacgttc actgaaaccg taaaacgtca cacaccggcg ataccggcct ccgggcaccg
    36241 cccgccagtc gtaatacgcc cggcagccaa atgtctgtgt caccaccatg accagacgaa
    36301 acatccacgc cggaactttt tcagcatcag aaggtgcccc ctgtgatgaa gaggtctgca
    36361 ccgaagaaag tccggcatcc tgctccgtga taccgtgttt cgccatcagc tgttgcgccc
    36421 ttgcaagcgc cagcccggcc tcatgggcat tgctgttatt acggctcagc tccagcagtt
    36481 tcctgacccg cctgatcaga cgttcctgat gcgacatatc actcatcatt ctcatccccc
    36541 tcatcgtcgt cgggtaatac actgcgctgc agggctcgca ggcggcgcag cacactcaga
    36601 agacgcagca gcttcacggc atggtcgtca tccagcagtg gccacgcttc cggtgcactg
    36661 ccccctgtaa gcaggtcttt cagactcacg gggaggactg tccgcggtgc atcaccggcc
    36721 agacccgcca gaaacgccac tacaggagaa catttctcca ctgctgcccc gaaaccggca
    36781 gacaggtcgc tttccctgtc catggcaatc tcttcagcac aaccggctga ttcagccagt
    36841 tcccaggcca gacggaacgc ttcattctgc agatgttcga tatcatcccg cctcacagca
    36901 aagtgcgtca ccgacgcggg aagtgcatca gtggttcccg gggcagtatc tcctgtcata
    36961 actgatgcca gtactgcccc ggcagcatca tcttccataa catcaccact gaagacaggc
    37021 ttaccaccgt acatatccgg ctgtatctca ggacgcaggg cattttcagc agaagacgcc
    37081 ccacagtccc ccctgcctgc gctctcttcc tgagaaggtc gactgcattt ttcagtgcgc
    37141 tctgtgtcag catcagaaac ggctttcagt ggccgggtaa taccatgttg cttgcgctca
    37201 gtaccgggga atatggagac tgtgctttcc ggcatcgcct ctgtatccgt atccggttga
    37261 cactccggta cttcagaccg gatcccggat accggtgccc cgggttgtac cgtactgctg
    37321 ccacggactg aaggaggcac caccaccggt tccggctcac caaaatgatg acgacggttt
    37381 cgctctttcg gatccagttc cagcaaccac cggtcatagt ccagggaggg atgtggcagg
    37441 gcctgcaaca aatctccgat aagctcatcg cggaacatct ccagtgacca cagttccggc
    37501 gaattaaact tccggcagct ttccccgaac acgcggtcaa attccgcatc cgtatcggct
    37561 gcaacagcaa actgccccca tacacgttct gcgtcctgac gaagtgcgag gagtgctgta
    37621 acctgtggac gccccagtcc tgactccatc aggtcgggga tccaggggta cagatacttc
    37681 agggcatctt ccattctgct gatcgtggag ttatgtatcg gcaacccttc atgggttaac
    37741 aaatcagcca gttcacgaag agacactgtt ctgcccagtt gttcctcatg gatttgtctt
    37801 gccttgtgga ccccctgcgc cttctcaata aaagtcagtt caccgcgaac ttcattttca
    37861 gccagatgac caatcacaca ctggagtcga cccggccagg gtttaaacag cacatgaata
    37921 cggtaaaaac gcggatcccc tgtttctttc cagagttccg tcagtatctg gtaacgggtg
    37981 tttcccccgt cactgaagat atacacatcc ggctcactgt ccggcatacg ggtgactttg
    38041 ggcaccgtat ccagcccgcg ggcatgaatg gaatttttga tatcgtcata acgcggattg
    38101 cgcgtggtgc gcgggttatc cggattcgga cgtaactggt ccagcgtcag aaccatgggc
    38161 gtttccccca ttgccaccac attgccggca ccagtggtct gacgaccggg ctgcataata
    38221 gccgccccga gatccagtgg tttatgtgcc atcacgcata tcctcccggc ccgtcattct
    38281 cagagggcgt aaacagtgtg taacgaccgt cgaacctgac ccgtaccgta cccgtgggcc
    38341 cctgacgctg cttgctgaca atgatttctg cctccccctt tgccagtgag tcggggttat
    38401 agacctcgtc gcggtaaata aacataatca ggtcagcatc ctgctccagc gccccggaat
    38461 cacgcaggtc accgttattg gggcgtttat cagcacgctg ctccacctgc cggttaagct
    38521 gggagagggc cagcaccggg cagcccagct ccttccccag cgctttcagg gaacgggaaa
    38581 tttcagcaat ttcctgtgtg cggttttcca gctccggcgc acgaatcagt tgcagataat
    38641 ccaccataat cagggagggt ttaccgtaca ggcgtgtata acgacgggcg cctgcacgca
    38701 gtgatgaagg cgtctgctca cagcagtcat caattattat gcgctcaccc agagagccca
    38761 ttcctccgtc accaagcgcc ataccgaccg cagatgacac cctcagccag tcctcatcat
    38821 ccatgttgcc actgcgcaaa tgattcagtt ccacacgccc cagcatggaa agcatgcgca
    38881 gaatcagttg ctccttcggc atctcaatgc tgaataccag aacgctctct tccgttcgcc
    38941 gcagtgcggc gctgagacac atggatagct ccagagaggt tttccccatt gacggacggg
    39001 cagccagcag aaccaggtca ccaggctgca ggccacaggt catttcatcc agacagtcaa
    39061 atcccgtggg ggtaccggta acaccaccgg agccgagcac actttccagt tgctgcagag
    39121 cggtatcaag cgcggatgaa agagaggctt ccgtgcgctc ccggagcgtc ctgttctgcg
    39181 caagcgcaaa cagacgccgc tctgcagcat ccagcacatc ccgtgatgtg gcgttcggtg
    39241 ccgccacatc agcaatcagg gtgtttccgg tggcctgtaa ctgacgcaga acgcttttct
    39301 ccaccacaat gcctgcatag gccatgatgt tggccgcaga aggtgtgttc ttgctgagct
    39361 cggccagata agcaaagccg cccagtacgt ccagcctgcc ctgatgttcc aggtgctctg
    39421 acagtgtaat caggtccagt ggctggccat tcaccgccag ttcagacatg gcacggaaaa
    39481 tagcccggtg ctgagccaca taaaaatcgt caggactcag catcagcacc acttcatccc
    39541 agcgttcatt ctccagcatc agcccaccca gaacggcctg ctctgcttcg tatgcacaga
    39601 gaacagacat ttcagccatg gcgtaccctc cctctctgac tgccggaaac ctggctacgt
    39661 gaaaaaaggg aaaagggtgc cggacgtaca gttgtcggga ataaaatccc ggtaactgtc
    39721 tgtacgtttt cagaccggtg agtttgccga ccccggtctg aaggtacgga cagcattcgt
    39781 tcattcataa caagttgttt taaaggaaaa agtttatgaa tacagaaaaa caactgccgt
    39841 ccggcattga atctgaggtg aaatacaccc tgcagttctg gctgctgagt aacggaaagg
    39901 tcatgacgga aaaagacggg ctccgtaacc cctttccccc gaactgtatt gaatcggctc
    39961 atccttttac tccgtttcag tagaatcagt ggattgaact ggacactgtc gggtcggatg
    40021 gaccggtgaa agcgacattc cttgtgcaca gcgttctgta taaactgaaa cgtcagggtg
    40081 gctccctgaa tgatgtcggc cggcctgaat acggccggtg cattgctcac tggcacactg
    40141 acgtgtttgt tgagaaaaaa gcgctgcctg actggctgag agcattccac actgacgttt
    40201 cgcactcgta aaaacacgtt cagcccgacg ggtcatattt tccagccacc cgtcggggaa
    40261 aatgtcagca ccatcacggg gaaacaaaat cgctttccat tcagggtatg agcgcggaat
    40321 acctccggaa gcattccggc tcagacagcg actcacagtg caggcagcac ggaatgcata
    40381 tgttgcgatg ctctcatcat atgttgcgat gttctcatta ttgcctttct gccgttccac
    40441 ccgctcttca gcatcaaatg ccagtgcagt aaggcttgcc cagcacatgc agttcactga
    40501 attccccgga acgctctgca gctcccggcg caccatgttg acctcagcac tgaaaaaagc
    40561 gttctcatcg ccttcaaggg cagccagatg cagcggaagc cagacctgac tgaaccattc
    40621 gcacatccgt tcccggttac tgacggtcat cactgtctcc gggcgggaac cgtcctgaaa
    40681 aacatgtccg gtgcgtggag aactgtatga aacataacca ccgtgcaccg caatgaccgg
    40741 acgacctgac gccggaagcc ccacacgaca cccactggca atccgggtca tcagcaggtt
    40801 cagttcagcc agtaattcag ggcgggttaa gaccgccgga accggagaat atttcactga
    40861 ggaagacata ctttcacctc catatctctc agcccgccag gtgcgtcact tccccgcaga
    40921 gaaccggaaa cctggctata tgaaaaaagg gaaaagggtg ccggacgaac agttgtcggg
    40981 aataaaatcc cggtaactgt ctgtacgttt tcagaccggt aagtttggcg accctggtca
    41041 gaaggtacgg acagcatctt tgcgctcata actgtaccga ccgggatatg cattacaaag
    41101 actgtcatac cgccccctcc gtctttcgct gtttgcagga aagaccacat gaaaaaagct
    41161 ggtcatgccg ctgacgacgc tggcgttcct gcaaaagaaa ggcgtcacaa ccggaacaca
    41221 gccccagacc actctcagga ttcaccttca cccagaccgg agcattctta ccggcaccat
    41281 cccgtacccc ggaataacca cagcccacgc acaccgtcca cttctgcagt acgatgagtg
    41341 cagtttcgag tgatgcgcgg agacgccggg aggcaataag gagcaacggc tcatggtcat
    41401 aaaaaacccc gccgttaccc cctccggatt caagcagcgg gagtcctgca ggctcccggg
    41461 gacctgttga catttcccgt aattcgtttt cattccacat attgtggaaa gttatccggt
    41521 tcactgaaca acgccattcc tccgccagac gctgatacaa atgctttttc gcttctgcgc
    41581 ggtcagatgc atcaacaaac acatgcactt cctcgtgtcc gggtgctgaa gccacggcac
    41641 gccatatcag ttttctgccc acaggctttt ccctccgttt tccagcagag catcaaaaaa
    41701 ctcacgccac tccggcaaca gttcacaggc cagactgtgc atggtgacca gcgcagaatc
    41761 gctgcaacgg gcagacactt tttccagacg atgcaccggc tgaccggctg cgtgcccctg
    41821 tttataaata ttcaggtcgt aaatctgtgt tgaaagaagg gtgaccgcac cttcaggcag
    41881 cactttgctg tagcgaccgg cgttgataat gcctgtcagc tctgccagtg tctgtctggc
    41941 cagcgccgta taatccatgc agttaaccag ggtgcggact ttcggcaggg gaatacccag
    42001 ttcacggtag ggcataaggt tttccatgac attgaccgtg ccgcggatga actcacgcac
    42061 atccggcaga accggattca ccatccccac cacagactcc gtggcggaca gcacaatcat
    42121 ttccagcatg acagaacgtg cgccctgtga atccaccaca atgacgtcat aggtctgaaa
    42181 aagcggatgc tgaagcacat tacgcagacg aaggcgacca tccggcgcat gaagcatggc
    42241 cgttttcagt tgctcatggg ggtcgttgga gacgatgagg tcaagccccc tgacagccgt
    42301 acgggaaatg atgttgtcag gatgactgag gtcagccgtg cgcatcagta attcaaaaag
    42361 accggccggc gcttcatacg tcagcggaaa gatattgctt gccgtgggct gtgcatggtc
    42421 tccgtctatc agcagagtac ggaggccggc atctgccaga aaaccggcaa gattagcaga
    42481 ctgggttgat tttccttcgc cacctttagt ggatataacc ggtaaaattt tcatatagca
    42541 cactctttaa agtatgcaac caatcactta cactacggaa accagccaga atattcactt
    42601 cactacctga atactcgcaa cacggggatt gaaaatcccc gtgtccttgg ttcgattccg
    42661 agtccgggca ccactttcac ttccagattc atctcccgaa ctccacgaaa ctcaagtata
    42721 atcacatcgt tacaagcaat ctgaacatcc tgaactccac cgaactctac tgaactcttt
    42781 ctcaatcaag ctttcattgg gggcaccggt gggggctcat tatgttcagt cagtttcagg
    42841 agcccccata tggcacttac agccagacag gtcgaaacag ccagacctaa agaaaaagac
    42901 tataaactct ctgacgaacg tggtttatat ctgctggtaa aaaccacggg agcccgctac
    42961 tggcggctta aataccggat agcaggaaaa gagaaaaaac tggccctcgg cgtctatccc
    43021 gacgtctccc ttgctgaggc cagaatcaaa cgtgacgatg cccgaaaaat catctccgaa
    43081 ggtggtgacc cgggcgaaaa gaagcgaaag gaaaaactca ctcagaaaat ctctgccacc
    43141 aatacgttcc atgccctcgc tacggaatgg caccagcata aatctttgtc atggtctgaa
    43201 agttacgcca gaagcgtact ggaagcgctg gataaagata ttttcccgta tctgggcaaa
    43261 cgaagcgtta cggatatcct cccgctggaa atgctggaaa ttctgcgccg catagaaaaa
    43321 cgtggctcgc tggaaaaact gcgtaaggta cgtcaatact gtaatcagat ttttcgttat
    43381 gccatcgcca ccggacgagc cactgtcaat ccggcatctg aactgaccag tacgctggcg
    43441 gcgccaaaag ctgcacactt cccccacctg agagcagatg agctccctgt ttttctcagg
    43501 aagctcgctg aatatcatgg cagtcctgtt acccgcatgg cgacaaatct gctgcttctg
    43561 acaggcctca gaacgattga gctacggtcc gctgaatggt cagaaattga ttttgataat
    43621 gccctgtgga caatccctga aagccgcatg aaaatgcgac gtaaacatgt cgtaccactg
    43681 tcacgacagg tcactgacat tctgcaacaa ctcaaaactt tctccggaca ataccggctg
    43741 gttttcccgg gacgttgtga tatcaacaag ccaatgagcg aagccagcat caatatggtg
    43801 ctcaaacgta tcggttacga tggcagagca accggtcatg gttttcgtca caccatgagt
    43861 accattctgc acgaacaggg gtttaattct gcctggattg aaatgcagtt agctcatgtg
    43921 gataaaaatt ccatcagggg tacctataat catgccctgt atattgatgg tcgccgtgaa
    43981 atgatgcagt ggtatgctga ctacattgat tcgctttctg agcaggtaag ttaagctgag
    44041 aacacgttat tctgattttc actcacttca cgattattcc tccggcctgc aggttcggag
    44101 gattcttatt tttatgtttt attttcagat aaagtttttg acaaaacgtt tattctgcat
    44161 cagcaaaatt atcacaaaac aacgcaatca cagacaaatt aacatttttc agacgggtat
    44221 attgagtgcc cacaatgtaa gacaggcagg catccggtga catggtaaaa tattaatcca
    44281 gggcaaaaac aaggcttgat acacaacatc atgactttta tgctctcaga ctatgccacc
    44341 acagatttta caggctcaga aaatgaatga cagattacgc tttgaagtga acgataacca
    44401 gggctgtttc gtgttcccgg acgcctggtt tggctcgttg ctggatgagt ttgacgaact
    44461 aattgatgca tacgacagag atgaaatcag cgaaacaagt tatatcaaca aactcagacg
    44521 ccttgcccga caggagcctg actttattga tgtacacgct tacctggcat gcgcatttct
    44581 ggagcagaat gccccgcgta aggcactgaa cgctgctctt aaaggactgg cagcaggtaa
    44641 tcgtctcatc ccggaaagtt tcagtggtga aatcatctgg atgcatcccg aaaaccggcc
    44701 ttatctgcgc gcgctttatg cagcaattct cgcaaatgtg cacttacagc gtcatcagga
    44761 tgcagctctg ctcactgata aaatactggc atacaatcct gaagacaatc agggagcacg
    44821 ctggctactg ggaccggaac tgttacgtac tggcgaccat gaacaggcac gccatgtact
    44881 gaaagcgcat gcagatgaat tttcacctta ctggtatgaa ctcggccttc tgcattttct
    44941 gaaccggccc cttgaaacgc cagacagtgg atgtatctta aaataagaga taggcttgga
    45001 tatatgtcta acactagttc taattttgag atgactggaa ttttgctggg gcaggagctt
    45061 cgtaaacgta aaactcctca ggaaaaaatc gccattattc agcagacaat ggaacccggt
    45121 atgacggtgt cccatgtggc gcgtttgcat ggtattcagc ccagcctgtt gtttaagtgg
    45181 aaaaaacaat atctggaagg aagtctcact gctgttgctg ccggagagga tgtcgttcct
    45241 gcctcagaac tggctgccgc tataaaacaa ataaaagagt tacagcgctt gctgggtaag
    45301 aaatctatgg tccccaccat ttttgcaaca ctgattttga ttgatgttgg cttgcctaaa
    45361 tctatccggc gtcattatgg gcatggatgc ccgcgcctcg atgagttccg cacctgatga
    45421 gcccaaaaaa ccatccggct tcagtgagcc attttcttgc tgaggctttt catcaggccg
    45481 atatgccgtt catgtcatca ataatcagtc ttcgcaaaac cagattggga actactttaa
    45541 ttcatgatta acctgccagc cagactggcg ccatcgttac ctttcgaaca cagtctgctg
    45601 tgttgtgatt gcccacgcta tccgggctaa tttgttggcc agtgcgcaac atactacgct
    45661 ggagtgtttt cttgccaaca acgccctgac ccaatccgcc aaccttcctg actgattctc
    45721 gattctctga ataaaggatc ttgcgcactg aacgagtaac cgtcgcagat ttttatctcc
    45781 acgtttactt atccctaaaa gtgtattttt accacctgtg ctgtactgcc ggggtaccag
    45841 accggttgaa gcagcaaagt cccgactgca gctatattgt ttaccgtctc ccagctgcga
    45901 tgacagtacg ctggcggtta tcggaccaat accgggtatt gtctgaagac gttgtcctac
    45961 ctcatcacga ctcagttcct gctccagtgc gatttcaagc tcggtgatct gtccgacaag
    46021 ataaatataa tgggcatgta agcgcatcag taactgcgcc agatagggag gaagttcgtt
    46081 ctctgcaagt actgttgaaa gtcgttttac cactgcgact cctgtcggca tactgatgcc
    46141 aaattccagc aaaaaagcat gcatctgatt gatcgttttg actttatcac tgaccaatga
    46201 ttccctgacc cggtgaagcg cccgcatggc ctgctgagct tctgttcgtg gttgcacaaa
    46261 tcgcatggag ggacgagatg ccgcttcgca tatggcttct gcatcgacaa aatcgttctt
    46321 gttgctctta acaaaagggc gaacgaactg cggagaaatc aattttgctt catgacctaa
    46381 atcagcaatc cggcgagcca taaaatgagc tccggcgcag gcttccatca caacggtaga
    46441 cgaggcacag cctgccagaa actcaataag tttggtacgg gtaaactttt tacgcagcaa
    46501 taccttgcct gacttatctt ggcagtgaat gtgaaaggaa tgcttgccaa gatctatacc
    46561 aataagcgta atgttttgca tgatggtctt ctccatgaaa gaaaaacact ctactagggt
    46621 acagctcaca gggtggtggg gaccatctca ttaaccatgg aagttgagat cctcaaagaa
    46681 gcggtggagt atggtcagtc gcgaaaatgg atagcgcatg cgcccttgtt gccaacggac
    46741 aaggaataac cctggtaagc cgtattctgg gcgtgtcgcg tgcgcaactg gcacaacgga
    46801 tgaaaacatc tggtaataag ccgaataaac gtcgtcgcag atgtgatgag gctgcggatg
    46861 cggaagtgtt agcacgtatc cttgatatca tcagcgatat gccgacatat ggctatcgtc
    46921 gtgtctgggc cattctcaga cgacagtccc gtaacaaagg agtgccgttc gtgaatgcaa
    46981 agcgcgttta tcggataatg agcgaaaata acctgttgtt acatgacaaa ccttcacggc
    47041 cgcagcggga acataaaggc cgaatatcgg tgaaggaaag cgaccagcgc tggtgttccg
    47101 atggctttga gttcggttgt gatgatggcg caaaactgcg tgtcacgttc gcactggact
    47161 gttgcgatcg tgaagccata gactgggccg caagcacggg aggttatgac aaagcaacgg
    47221 tgcaggatgt catgttagga gcaatagaaa aacgctttgg cgataaagga gcggaacggc
    47281 caatccagtg gctgactgat aacggttcgg cttatacagc tcatgaaaca cggcagttcg
    47341 ccagagagtt gaatctggag ccctgcatga cggcagtcag tagccctcag agcaatggaa
    47401 tggcagaaag gttcgtgaaa acgatgaaag aagactacat cgcgttcatg ccgaaaccga
    47461 atgtaagaac ggcattgcat aaccttgcag tggcgatcga gcattacaat gaaacccatc
    47521 cgcatagtgc gttaggttat ctctcaccaa gggaatatcg acgtcagcgg gtaacgtcaa
    47581 cttaagatac aacgactgtc tgaatatcgt gggtcaagga cagccttctg cattttctta
    47641 atggggaact ggtaaaagct gcaactgcat ttcgtcgcgg ctttgcggca aatacttaca
    47701 tcgcagaaat actgtgcgga aatctgcatc catttccact tgctgtatgg cataactttt
    47761 caggcggtcc ggatactgct gaagattatt atgccacata ccacccgtta tgggggcaat
    47821 atccggaagc attgctgttt gtaaactggc tctataatca ttcctctgtg ctgcatgaac
    47881 gggcagaaat cattaaatgc gccgaaatgc tgatgcagga agatgatttc gaaatatgcg
    47941 aaagtatttt aagacagcag gagaagttgc gtgaaagaat tggtgagacg ctttctgaga
    48001 aaattgtaca gaaatgcaga aatatgaatg gtgaatatgt ctggccctgg atattgccgt
    48061 tttcagcggc aggcatgaaa catactggca tacagtatca gtagatattg cattagtgta
    48121 tcctgcacac aagtaataat ttatccacca ataataacac tgttaatgtc cccttcccct
    48181 ggttgtcagc caggggttat cttctgaata tttcttttga aaaggataac acagtaaatt
    48241 atttttatga attatcccat ggactcatta acaccctttc ataatgcttt attgtcaaac
    48301 acgttatggc tgacatcaaa aaaaccggat ttcctctgcc agcgggtaat cacctccccg
    48361 gtgttttcgg ttggtctggt tactccagtc tggtaattag caagataatc gatataaaca
    48421 gtggaaaact catcgtacat aatctggtga tgaacattac gcttattttc ccctgaccgg
    48481 aagaatcaga ggctgcggtt tcagactgtc tgccggtaca ttcctctccc cgttaaaaac
    48541 cataacgggt tcattatctt cgtctgtcag cggattgaat ggcggtatat tttcagtacg
    48601 aatgccggtc agccactgaa aaatacctgc gaaatgacgg gcactgattt tcctgctgac
    48661 ggactgatga gacgtgatgt cactggcggt aataatcagg ggaacgctgt agcctccctg
    48721 cacatgacca tcatgatgaa caggattggc actatcgctg accgacagcc catggtcaga
    48781 aaagtaaagc atggcgaaat gacgggaatg ccggcgaagg ataccatcaa gctgaccgag
    48841 aaagttatcc agtttactga tgctggcgag gtaacaggca atctttcggg gatactgctc
    48901 caggtaatga ttcggccagg agttaagtcg gtcacacgga ttcggatgag agccaatcat
    48961 gtgcaggaaa atcaccttcg gagaggattt atccgccagc gcacgttctg tttcctgtaa
    49021 caacaacatg tcatccgttt tacgggaagc aaagctgcct ttcttgagga aaacggtatg
    49081 ctccgcatca gaagcaataa cagagatgcg tgtatcatgc tcccccagtt ttccctgatt
    49141 ggatatccac catgtgctgt atcctgcttt tgctgccagc gctaccacgt tgttgccgga
    49201 gtcagggttc tgctcatagt cataaatcag tgtccggctc agggaaggta cggtactggc
    49261 tgctgccgat gtatagccgt caataaataa accgggagcc gtattcagcc acggtgtggt
    49321 tggtacggga taaccatata ccgacatata atccctgcgc acactctcac cagtgacaat
    49381 cacaatcgtg tcatacaacg gtgctcccgg caggattttc cagctgtcag ccctgtgctg
    49441 actcagttgt tcataacgct gcatttcacg caatgtgtca gttgtcccca caacggttcc
    49501 tttaaccatc cgcaacggcc agctgtttac agagcataat acgaacagca gcagtgccag
    49561 ccagttacgg tgaccgcggt ggtgtgtccg ccagaaaata accatgaaca ccagaatcgc
    49621 ggcactgacc agaaaatgat aaacaggaat catcccggta aactccgcag cctcatccgc
    49681 cgtggtctgc aacaacgcga caataaaact gttgttgatt ttaccgtacg tcatacccgc
    49741 aggcgcatac agtgcacaac agaacagaaa taacagagct gtaatggatg taagggtatt
    49801 tctgtgtgca aggagcagaa gaaggaacag cagcaacaca ttcccggtgg cattcttctc
    49861 agtgtatccg catgcaattg tggttatgac agaaataaca aaaaagaata aaaacaataa
    49921 aatccgtggt gtgttgtccg gacaaaacag ttttctgata ttcatcggag tatatcggca
    49981 acattattat gaagagaata aaataataag aaaaacagaa atatgctgta aagcagataa
    50041 cagacaaccc tgcagtataa ttacggcagg gagtttccgt ttaattacag aagacaggta
    50101 attattatct ttcaggagta gtttcgtcag aagttataag tcacaccaag catgatgtca
    50161 tgacttttaa catcaacttc tgatttatat ttatcgcccc actcatcctt gtaactcaca
    50221 ctggcatcac ctgcatcaag atagcgatag ctgaggtcca gagcgatatc cggggttacg
    50281 tcatagcgga cacctgcccc aaaactccat gcaaaattgt tatcatatcc cgggtaatgc
    50341 aacgcagaaa aattgctcac ctcaccagat ttattccagg aggtatcagt atatgttgcc
    50401 ttatgatgta tccgtgcata acccagtcca acagacaccc atggagtgaa tgcactgctg
    50461 gtgaatgcac tgctgtttct gaagtcataa tacgtattca ccatcagagt attcacactg
    50521 agcctgttct gtgtgtcttc ccgaccacca ccgaccatcg gagagtacca tgtatccagt
    50581 gtataacggg agtccgcagc tccgcggcca tagaattcca cttccgtgcg cacaggaaca
    50641 ttgtaatgct gataaaaatc atagcccacc gcaaggccgg caccgaatac cgtatcgttc
    50701 ttatcgccgc ccttatactt ataaacatcg tccccaaagt cctgacgaaa gcgctggtca
    50761 gtctgagtca caaccgaagc accagcttta ccggtcatat aaaagcctgt tttgcctcta
    50821 tcagccaggg ccaggcctga aaacatgccc accacagcaa gcgctgaaac cgcaataatc
    50881 tttttcattt cagttatctc cgctatcact ttccccgaac gataaacgtg gttgcttatt
    50941 ctaaagctat gcaaaagaca gggtaaaaag agaaaaatcg atcgaatcga tcgacaaaat
    51001 cgatcatgca tgatgaagat ttcttatcga atacataaaa atagtgatgg caatcgtggc
    51061 aggagcttac gtcaggccct gaagtactga cacaggcatt gatggcaggt gatataagga
    51121 gtgctccagg gaatgactcc cgactgaccc aggcactgtc ccgttgtcaa cccgtatggt
    51181 tactgctctg actcatttcc tgtcgtcaga cgttcaactc tggaccactg gttatgacga
    51241 caggtgtcgc tcatttgtca gcactataac ggtcaaagat cgtggcattg acacattctc
    51301 cagaggtaaa tttctcaaga ggtaacaact ctgccaggag gatatctgca gagagttaca
    51361 ttcgtttggg ttcatatctg tgccgcgcgt tttccttcca tcagtgaaca aacattcagt
    51421 cccatgacga ccgggcaaat agcgtgttca cagatattgc tgtccaggta cgaagtacca
    51481 taatggatat tggtaagtta ttgggtgaac cccctccatg aaaaaaccaa atataaaaat
    51541 gaatgtttcg ggagagactt cactcgcttt attgatagtg attgtttatc ttttactgaa
    51601 gacaggatat ttcagatgtg atatccctgt aacatattga ccagcaatac tttttatttg
    51661 taccactgga ggatattgtg ctgaaaaatt tacggctgca tattctgttc ctgcttttta
    51721 tctcagtgtc agtcagggcg gaaaaaccat ggtattttga tgcaattggt ctgacagaaa
    51781 caaccatgtc gctcacagac aaaaataccc cggttgtcgt atctgttgtt gattccggag
    51841 tggcatttgt aggaggcctc agcgacagcg aatttgccaa attcagtttt actcaggatg
    51901 gttcgccatt cccggtaaaa gagcctgaag ctttatatat tcatggtact gccatggctt
    51961 cactcattgc ctcacgtcat gaggtttatg gtgtttatcc tcatgcactg atatccagca
    52021 gaagggttat tcctgacggt gtacaggact catggattag agcaactgaa agcattatgt
    52081 caaatgtttt tcttgcacca ggagaagaga aaatcattaa tatatcggga ggccagaagg
    52141 ggatatcttc cgcatcggtc tggtccgaac tgctttcccg tatgggccgg aataatgagc
    52201 gattaattgt tgcggcagtg ggtaatgatg gcgcagatat acgaaaaatg agtgctcagc
    52261 agagaatatg gcctgcggct taccatccgg tcagcagtgt gaataaaaag caggatcctg
    52321 tgataagagt ggcagctctg gcacagtacc ggaaaggaga aacacctgta ttgcatggtg
    52381 gaggggtcac cggaagtcgg ttcggaaacg gctgggttga tattgccgca ccgggacaga
    52441 atattacatt cctcagacca gatggaaaaa cggggatcgg tagtggaacc tcggaagcaa
    52501 cagctatagt ttccggcgtt ctggcagcga tggtctcatg taatccccgt gcaacagcga
    52561 cagaactgaa gcgtacactg ctggagtctg cagacaaata cccctcactt gcagataagg
    52621 tgacggaagg gagagttctt aatgcagaaa aagcaattag catgttctgc aagaaaaatt
    52681 atattcctgt ccgtcaggga aggatgagtg aagaactgta aaattccgga ggtgaatatg
    52741 acgattaaga ggttttttgt atgtgcaggt gttatgggat gtttatccct taatcctgcc
    52801 atggctgagt ggactggcga tgcccgggat ggcatgtttt caggcgttgt tattgatcag
    52861 tttcatacag ggcaaataga taataaccct tatttttgta ttgaagggaa acaacctggt
    52921 ggttcttcca taagtgcctg ctcgatgaag aattcatctg tctgggggcc ttcgttttcc
    52981 acattatata atcaggcact atatttttac acaacaggtc agttggtcag gatttattat
    53041 gaacccgggg tatggacgta tccgcccttt gtgaaggcat taacgtccaa tgcccttgtt
    53101 gggttatcaa cctgcgccac atcgacagaa tgttttggcc ccgacaggaa aaagaactaa
    53161 taaatgatgc tcctgcccag atatatactg ggcgggagca tactttggtt gcgacagaaa
    53221 tatgtcaaca ccacgaactt tgacattacg tcttatatca cactggcact gacaagccag
    53281 tattgctaat gcaggaggat gctttttcaa ctcgcaggag tttcaggagc tgcctgatgg
    53341 cgctgaaatg atgcttactc atgtggtggc tggtcactgc ccctgtccgc gagctggcac
    53401 gcaatgcact actgatggca gtctggcgcg taagcctgca taaaacatta ttgtactatg
    53461 tgtatcaacg ggttacccgg ttagaacagc cagtacaaaa atgcccccgc agtgagcatg
    53521 gcaagttttc cccatacaat gggtttctca cccctctttt gttttctttt tctcatataa
    53581 atgatggtgt ttactcagac ggaagagtat ttcg
//
LOCUS       DAZFKF010000025        50520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFKF010000025 DAZFKF010000000
VERSION     DAZFKF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.025"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(101..1465)
                     /gene="rhlE"
                     /locus_tag="ACHKA1_001827"
     CDS             complement(101..1465)
                     /gene="rhlE"
                     /locus_tag="ACHKA1_001827"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO5679485.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQLRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            1694..2365
                     /gene="cecR"
                     /locus_tag="ACHKA1_001828"
     CDS             1694..2365
                     /gene="cecR"
                     /locus_tag="ACHKA1_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO5679486.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2368..3363
                     /gene="hlyD"
                     /locus_tag="ACHKA1_001829"
     CDS             2368..3363
                     /gene="hlyD"
                     /locus_tag="ACHKA1_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO5679487.1"
                     /translation="MKKPVVIGLAVVALAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3356..5092
                     /gene="ybhF"
                     /locus_tag="ACHKA1_001830"
     CDS             3356..5092
                     /gene="ybhF"
                     /locus_tag="ACHKA1_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO5679488.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5085..6218
                     /gene="ybhS"
                     /locus_tag="ACHKA1_001831"
     CDS             5085..6218
                     /gene="ybhS"
                     /locus_tag="ACHKA1_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5679489.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6229..7335
                     /gene="ybhR"
                     /locus_tag="ACHKA1_001832"
     CDS             6229..7335
                     /gene="ybhR"
                     /locus_tag="ACHKA1_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5679490.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTYQTVPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7297..7707)
                     /gene="ybhQ"
                     /locus_tag="ACHKA1_001833"
     CDS             complement(7297..7707)
                     /gene="ybhQ"
                     /locus_tag="ACHKA1_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO5679491.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7840..8601
                     /gene="ybhP"
                     /locus_tag="ACHKA1_001834"
     CDS             7840..8601
                     /gene="ybhP"
                     /locus_tag="ACHKA1_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO5679492.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8598..9839
                     /gene="clsB"
                     /locus_tag="ACHKA1_001835"
     CDS             8598..9839
                     /gene="clsB"
                     /locus_tag="ACHKA1_001835"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO5679493.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9839..10795
                     /gene="ybhN"
                     /locus_tag="ACHKA1_001836"
     CDS             9839..10795
                     /gene="ybhN"
                     /locus_tag="ACHKA1_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO5679494.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGENTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10831..11544)
                     /gene="ybhM"
                     /locus_tag="ACHKA1_001837"
     CDS             complement(10831..11544)
                     /gene="ybhM"
                     /locus_tag="ACHKA1_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO5679495.1"
                     /translation="MESYLQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAFIGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(11750..12454)
                     /gene="ybhL"
                     /locus_tag="ACHKA1_001838"
     CDS             complement(11750..12454)
                     /gene="ybhL"
                     /locus_tag="ACHKA1_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO5679496.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDSSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(12590..13042)
                     /gene="moaE"
                     /locus_tag="ACHKA1_001839"
     CDS             complement(12590..13042)
                     /gene="moaE"
                     /locus_tag="ACHKA1_001839"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO5679497.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13044..13289)
                     /gene="moaD"
                     /locus_tag="ACHKA1_001840"
     CDS             complement(13044..13289)
                     /gene="moaD"
                     /locus_tag="ACHKA1_001840"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO5679498.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(13282..13767)
                     /gene="moaC"
                     /locus_tag="ACHKA1_001841"
     CDS             complement(13282..13767)
                     /gene="moaC"
                     /locus_tag="ACHKA1_001841"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO5679499.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(13770..14282)
                     /gene="moaB"
                     /locus_tag="ACHKA1_001842"
     CDS             complement(13770..14282)
                     /gene="moaB"
                     /locus_tag="ACHKA1_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO5679500.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14304..15293)
                     /gene="moaA"
                     /locus_tag="ACHKA1_001843"
     CDS             complement(14304..15293)
                     /gene="moaA"
                     /locus_tag="ACHKA1_001843"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO5679501.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15281..15425)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15690..16598
                     /gene="yvcK"
                     /locus_tag="ACHKA1_001844"
     CDS             15690..16598
                     /gene="yvcK"
                     /locus_tag="ACHKA1_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO5679502.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(16789..18810)
                     /gene="uvrB"
                     /locus_tag="ACHKA1_001845"
     CDS             complement(16789..18810)
                     /gene="uvrB"
                     /locus_tag="ACHKA1_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO5679503.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(19389..20066)
                     /gene="bioD"
                     /locus_tag="ACHKA1_001846"
     CDS             complement(19389..20066)
                     /gene="bioD"
                     /locus_tag="ACHKA1_001846"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO5679504.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRSIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPRLAENPENAATGK
                     YINLALL"
     gene            complement(20059..20814)
                     /gene="bioC"
                     /locus_tag="ACHKA1_001847"
     CDS             complement(20059..20814)
                     /gene="bioC"
                     /locus_tag="ACHKA1_001847"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO5679505.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLVWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYRHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            complement(20801..21955)
                     /gene="bioF"
                     /locus_tag="ACHKA1_001848"
     CDS             complement(20801..21955)
                     /gene="bioF"
                     /locus_tag="ACHKA1_001848"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO5679506.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVTQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFIHNDVAHLAR
                     LLASPCSGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTSAHEMQDIDRLLEVLHGNG"
     gene            complement(21952..22992)
                     /gene="bioB"
                     /locus_tag="ACHKA1_001849"
     CDS             complement(21952..22992)
                     /gene="bioB"
                     /locus_tag="ACHKA1_001849"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO5679507.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            23079..24368
                     /gene="bioA"
                     /locus_tag="ACHKA1_001850"
     CDS             23079..24368
                     /gene="bioA"
                     /locus_tag="ACHKA1_001850"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO5679508.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEAQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIIHPQQLQRLTAGVNRAVQDETFFCQ"
     gene            24427..24903
                     /gene="ybhB"
                     /locus_tag="ACHKA1_001851"
     CDS             24427..24903
                     /gene="ybhB"
                     /locus_tag="ACHKA1_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO5679509.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            25055..26338
                     /gene="ybhC"
                     /locus_tag="ACHKA1_001852"
     CDS             25055..26338
                     /gene="ybhC"
                     /locus_tag="ACHKA1_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO5679510.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(26479..28740)
                     /gene="ybhJ"
                     /locus_tag="ACHKA1_001853"
     CDS             complement(26479..28740)
                     /gene="ybhJ"
                     /locus_tag="ACHKA1_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO5679511.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(28923..30356)
                     /gene="ybhI"
                     /locus_tag="ACHKA1_001854"
     CDS             complement(28923..30356)
                     /gene="ybhI"
                     /locus_tag="ACHKA1_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO5679512.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(30432..31484)
                     /gene="ybhH"
                     /locus_tag="ACHKA1_001855"
     CDS             complement(30432..31484)
                     /gene="ybhH"
                     /locus_tag="ACHKA1_001855"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO5679513.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIIPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            31668..32621
                     /gene="ybhD"
                     /locus_tag="ACHKA1_001856"
     CDS             31668..32621
                     /gene="ybhD"
                     /locus_tag="ACHKA1_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5679514.1"
                     /translation="MKHELSSMKAFVILAESNSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFS
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(32662..33657)
                     /gene="pgl"
                     /locus_tag="ACHKA1_001857"
     CDS             complement(32662..33657)
                     /gene="pgl"
                     /locus_tag="ACHKA1_001857"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO5679515.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            33812..34630
                     /gene="ybhA"
                     /locus_tag="ACHKA1_001858"
     CDS             33812..34630
                     /gene="ybhA"
                     /locus_tag="ACHKA1_001858"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO5679516.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(34631..35689)
                     /gene="modC"
                     /locus_tag="ACHKA1_001859"
     CDS             complement(34631..35689)
                     /gene="modC"
                     /locus_tag="ACHKA1_001859"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO5679517.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(35692..36381)
                     /gene="modB"
                     /locus_tag="ACHKA1_001860"
     CDS             complement(35692..36381)
                     /gene="modB"
                     /locus_tag="ACHKA1_001860"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO5679518.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(36381..37154)
                     /gene="modA"
                     /locus_tag="ACHKA1_001861"
     CDS             complement(36381..37154)
                     /gene="modA"
                     /locus_tag="ACHKA1_001861"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5679519.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(37321..37470)
                     /gene="acrZ"
                     /locus_tag="ACHKA1_001862"
     CDS             complement(37321..37470)
                     /gene="acrZ"
                     /locus_tag="ACHKA1_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO5679520.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            37599..38387
                     /gene="modE"
                     /locus_tag="ACHKA1_001863"
     CDS             37599..38387
                     /gene="modE"
                     /locus_tag="ACHKA1_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO5679521.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            38455..39927
                     /gene="modF"
                     /locus_tag="ACHKA1_001864"
     CDS             38455..39927
                     /gene="modF"
                     /locus_tag="ACHKA1_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO5679522.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYHYALTKIN"
     gene            40189..41205
                     /gene="galE"
                     /locus_tag="ACHKA1_001865"
     CDS             40189..41205
                     /gene="galE"
                     /locus_tag="ACHKA1_001865"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO5679523.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPMFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMCAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            41215..42261
                     /gene="galT"
                     /locus_tag="ACHKA1_001866"
     CDS             41215..42261
                     /gene="galT"
                     /locus_tag="ACHKA1_001866"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO5679524.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQVWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            42265..43413
                     /gene="galK"
                     /locus_tag="ACHKA1_001867"
     CDS             42265..43413
                     /gene="galK"
                     /locus_tag="ACHKA1_001867"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO5679525.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            43407..44447
                     /gene="galM"
                     /locus_tag="ACHKA1_001868"
     CDS             43407..44447
                     /gene="galM"
                     /locus_tag="ACHKA1_001868"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO5679526.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALTSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHLWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLHPGEE
                     YSSLTEYQFIAE"
     gene            44649..45401
                     /gene="gpmA"
                     /locus_tag="ACHKA1_001869"
     CDS             44649..45401
                     /gene="gpmA"
                     /locus_tag="ACHKA1_001869"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO5679527.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(45559..46611)
                     /gene="aroG"
                     /locus_tag="ACHKA1_001870"
     CDS             complement(45559..46611)
                     /gene="aroG"
                     /locus_tag="ACHKA1_001870"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO5679528.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            46927..47307
                     /gene="ybgS"
                     /locus_tag="ACHKA1_001871"
     CDS             46927..47307
                     /gene="ybgS"
                     /locus_tag="ACHKA1_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO5679529.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            47421..48362
                     /gene="zitB"
                     /locus_tag="ACHKA1_001872"
     CDS             47421..48362
                     /gene="zitB"
                     /locus_tag="ACHKA1_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO5679530.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(48359..49078)
                     /gene="pnuC"
                     /locus_tag="ACHKA1_001873"
     CDS             complement(48359..49078)
                     /gene="pnuC"
                     /locus_tag="ACHKA1_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO5679531.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALTWLAVCVIAIGLMTVFINPVFAFLTRVAVIIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(49116..50159)
                     /gene="nadA"
                     /locus_tag="ACHKA1_001874"
     CDS             complement(49116..50159)
                     /gene="nadA"
                     /locus_tag="ACHKA1_001874"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO5679532.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(50437..>50520)
                     /locus_tag="ACHKA1_001875"
     ncRNA           complement(50437..>50520)
                     /ncRNA_class="other"
                     /locus_tag="ACHKA1_001875"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT        12233 a        13404 c        12877 g        12006 t
ORIGIN      
        1 aatttattaa aacaataaaa acagacatgt catgcatgtc tgaccgtgtt acctgaataa
       61 agggcaaaag cctgtgatac tcttttgccc ttacatggta ttactgcgca gcggcaggtt
      121 tacgcggacg gcggcgacgt tgttgttcgc ctgccggttt ggcatcgccg aggcggcgag
      181 acggtttttc tgcaggcttc gcgcttgcag attttgcacc accttctcca cggcgtagtt
      241 gctgttgacc gcgaccaccg ccctgcccac gaccaccgcc gccacgttgc tggcgaccgt
      301 tctggatcgg ttcggctttg attgacgggt ccggctcata gcccggaatc gcaatgcgcg
      361 ggatctcttt tttcagcagt ttttcgatat cacgcagcag tttgtgttca tcaacacaca
      421 ccaacgacaa cgcttctccg gtggctgcgg cacgaccggt acgcccgata cggtggacat
      481 aatcttccgg tacgtttggc agttcatagt tgacgacgtg cggcaactct tcgatatcca
      541 gaccgcgcgc agcgatgtca gttgccacca gtacacgaat atcgcccgat ttaaaatcag
      601 ccagcgcacg ggtacgcgca ccttgcgatt tattaccgtg gatcgccgca ctacggatgc
      661 catctttatt aagctgttca gcgagatggt tagcgccatg tttggtacgg gtaaacacca
      721 gcacctgctg ccagttccct ttcccaatca tgtgcgacag caattcgcgt ttgcgcttct
      781 tatcaacaaa gtgaacatgc tgggtcacct gatcagacgc ggtattgcgg cgtgccactt
      841 cgatttccag cgggttgtgc aacagttttt ccgccagggc tttaatatcg tcagagaagg
      901 tcgcggagaa caacaggttc tggcgcttcg caggtagttt tgttaacacg cgacggatat
      961 cgtggataaa gcccatgtcg agcatgcggt cagcttcatc gaggacgagg atttcaacct
     1021 gatccagctt cactgcattc tgatgttcca ggtccagcaa acgtcccggc gttgccacca
     1081 gcacatcaac gccgccgcgc aacttcatca tttgtgggtt aatgctgaca ccgccaaaca
     1141 ccaccagcga acgaatgttc agatatttgc tgtaatcacg gacgttttcg ccaatctgcg
     1201 cagccagttc acgggtcggg gtaagaatga gagcacgtac cggacgacgc cctttggcgt
     1261 gcggctggcg agtgatcagg tgttgcaaca gcggcagcgt aaagcccgct gttttgccgg
     1321 tgccggtctg ggcgctagcc atcaggtcgc ggccttccag caccgcaggg atcgcctgct
     1381 gctgaatagg ggtgggttca cggtaaccct gctcggcaac ggcgcgcagg atatcagggc
     1441 ttaaacccaa agaatcgaaa gacataacta ctccgaaccg ccccgaccgt taccggtgta
     1501 gttttcaggg agatactgaa aaggaaaatg acaaaaacca caatgtcatg agggagcgga
     1561 gtgtagcagt ttttgtgacg cagcgcataa attatccctg tgactggcgt agctaaaatt
     1621 cttagccata ctggacaact cccctatgga gtcataatct taatcaatca tttgattaag
     1681 aatggactgt gcgatgaata atcctgccat gacaatcaag ggtgaacagg cgaaaaaaca
     1741 gctgattgct gccgcactgg cgcagtttgg tgaatatgga atgaacgcca ccacccgcga
     1801 gatagccgcc caggccgggc agaatatcgc tgccatcacc tactacttcg gttcgaaaga
     1861 agatttgtac ctcgcctgcg cccagtggat tgccgatttt attggcgagc agttccgtcc
     1921 gcatgccgag gaagccgaac gcttgttcgc ccaaccacag cctgatcggg ctgccatccg
     1981 tgaactgatc cttcgcgcct gcaggaacat gattaagctg ctcacccagg atgacaccgt
     2041 caacctcagc aagtttatct cccgtgagca gctctctccc acggcagcct accacctggt
     2101 gcacgaacag gtgattagtc cgctacacag ccacctgaca cgcctgattg ccgcctggac
     2161 cggctgcgac gccaatgaca cccgcatgat ccttcatacc catgcgttga ttggtgagat
     2221 tctggcgttc cgtcttggca aagaaacaat tctgttacgt accggctgga ccgcgttcga
     2281 tgaagaaaag accgaactga tcaaccagac ggtgacctgt catatcgacc tgattttgca
     2341 aggattatcg caaaggagtt tgtagtgatg aaaaaacctg tcgtgatcgg actggcggta
     2401 gtggcacttg ccgccgtggt tgccggaggc tactggtggt atcaaagccg ccaggataac
     2461 ggcctgacgc tgtatggcaa cgtggatatt cgtacggtga atcttagttt ccgtgttggg
     2521 gggcgcgttg aatcgctggc ggtggacgaa ggtgatgcga tcaaagcggg ccaggtgctg
     2581 ggcgaactgg atcacaagcc gtatgagatt gccctgatgc aggcgaaagc gggcgtctcg
     2641 gtggcacagg cgcagtatga cctgatgctt gccgggtatc gcgatgaaga aatcgctcag
     2701 gccgccgctg cggtgaaaca ggcgcaagcc gcctatgact atgcgcagaa cttctataac
     2761 cgccagcaag gactgtggaa aagccgcacc atttcggcaa atgacctgga aaatgcccgc
     2821 tcctcgcgcg accaggcaca ggcaacgctg aaatcagcac aggataaatt gcgtcagtac
     2881 cgttccggta accgtgaaca ggacatcgcc caggcgaaag ccagcctcga acaggcgcag
     2941 gcgcaactgg cgcaggcgga gctgaattta caggactcaa cgttgatagc accgtctgat
     3001 ggcacgctgt taacgcgcgc ggtggagcca ggcacggttc tcaatgaagg tggcacggtg
     3061 tttaccgttt cactaacgcg tccggtgtgg gtgcgcgctt atgttgatga acggaatctt
     3121 gaccaggccc agccggggcg caaagtgctg ctttataccg atggtcgccc ggacaagccc
     3181 tatcacgggc agattggttt cgtttcgccg actgctgaat ttaccccgaa aaccgtcgaa
     3241 acaccggatc tgcgtaccga cctcgtctat cgcctgcgta ttgtggtgac cgacgccgat
     3301 gatgcgttac gccagggaat gccagtgacg gtacaattcg gtgacgaggc aggacatgaa
     3361 tgatgccgtt atcacgctga acggcctgga aaaacgcttt ccgggcatgg acaagcccgc
     3421 cgtcgcgccg ctcgattgca ccattcatgc cggttatgtg acggggctgg tagggccaga
     3481 cggtgcgggt aaaaccacgc tgatgcggat gctggcggga ttactgaaac ctgacagcgg
     3541 cagtgccacg gtgattggct ttgatccgat caaaaatgac ggcgcgctgc acgccgtgct
     3601 cggctatatg ccgcagaaat ttggtctgta tgaagatctg acggtgatgg agaacctcaa
     3661 tctgtacgcg gatttgcgca gcgtcaccgg cgaggcacgt aagcaaactt ttgctcgcct
     3721 gctggagttt acgtctcttg gcccgtttac cggacgcctg gcgggcaagc tctccggtgg
     3781 gatgaaacaa aaactcggcc tggcctgtac cctggtgggc gaaccgaaag tgttgctgct
     3841 cgatgaaccc ggcgtcggcg ttgaccctat ctcacggcgc gaactgtggc agatggtgca
     3901 tgagctggcg ggcgaaggga tgttaatcct ctggagtacc tcgtatctcg acgaagccga
     3961 gcagtgccgt gacgtgttac tgatgaacga aggcgagttg ctgtatcagg gagaaccaaa
     4021 agccctgaca caaaccatgg ccggacgcag ctttctgatg accagtccac acgagggcaa
     4081 ccgcaaactg ttgcaacgcg ccttgaaact gccgcaggtc agcgacggca tgattcaggg
     4141 gaaatcggta cgtctgatcc tcaaaaaaga ggccacacca gacgatattc gccatgccga
     4201 cgggatgccg gaaatcaaca tcaacgaaac tacgccgcgt tttgaagatg cgtttattga
     4261 tttgctgggc ggtgccggaa cctcagaatc gccgctgggt gcaatattgc atacggtaga
     4321 aggcacaccc ggcgagacgg tgatcgaagc gaaagaactg accaagaaat ttggtgattt
     4381 tgccgccacc gatcacgtca actttgccgt taaacgcggg gagatttttg gtttgctggg
     4441 gccaaacggc gcgggcaaat cgaccacctt taagatgatg tgcggtttgc tggtgccgac
     4501 ttccggccag gcgctggtgc tggggatgga tctgaaagag agttccggta aagcgcgcca
     4561 gcatctcggc tatatggcgc aaaaattttc gctctacggc aacctgacgg tcgaacagaa
     4621 tttacgcttt ttctctggtg tgtatggctt acgcggtcgg gcgcagaacg aaaaaatctc
     4681 ccgtatgagc gaggcatttg gcctgaaaag tattgcctca cacgccaccg atgaactgcc
     4741 attaggtttt aaacagcggc tggcgctggc ctgttcgctg atgcatgaac cggatattct
     4801 gtttctcgac gaaccgactt ccggcgttga ccccctcacc cgccgtgaat tctggctgca
     4861 catcaacagc atggtagaga aaggcgtcac ggtgatggtc accacccact ttatggatga
     4921 agcggaatat tgcgaccgca tcggcctggt gtaccgcggg aaattaatcg ccagcggcac
     4981 gccggacgat ttgaaagcac agtcggctaa cgatgagcaa cccgatccca ccatggagca
     5041 agcctttatt cagttgatcc acgactggga taaggagcat agcaatgagt aacccgatcc
     5101 tttcctggcg tcgcgtgcgg gcgctgtgcg tcaaagagac gcggcagatc gttcgcgatc
     5161 cgagtagctg gctgattgcg gtagtgatcc cgctgttgct gctgtttatt tttggttacg
     5221 gcattaacct cgactccagc aagctgcggg tcgggatttt actggaacag cgtagcgaag
     5281 cggcgctgga tttcacccac actatgaccg gttcgcccta catcgacgcc accatcagcg
     5341 ataaccgtca ggaactgatc gccaaaatgc aggcggggaa aattcgcggt ctggtggtta
     5401 ttccggtgga ttttgcggaa cagatggagc gcgccaacgc caccgcaccg attcaggtga
     5461 tcaccgacgg cagtgagccg aataccgcta actttgtaca ggggtatgtc gaagggatct
     5521 ggcagatctg gcaaatgcag cgagcggagg acaacgggca gacttttgaa ccgcttattg
     5581 atgtacaaac ccgctactgg tttaacccgg cggcgattag ccagcacttc attatccccg
     5641 gtgcggtgac catcatcatg acggtcatcg gcgcgattct cacctcgctg gtggtggcac
     5701 gagaatggga acgcggcacc atggaggctc tgctctctac ggagattacc cgcacggaac
     5761 tgctgctgtg taagctgatc ccttattact ttctcgggat gctggcgatg ttgctgtgta
     5821 tgctggtgtc agtgtttatt ctcggcgtgc cgtatcgcgg ttcgctgctg attctgtttt
     5881 ttatctccag cctgttttta ctcagtaccc tggggatggg gctgctgatt tccacgatta
     5941 cccgcaacca gttcaatgcc gctcaggtcg ccctgaacgc cgcgttcctg ccgtcgatta
     6001 tgctttccgg ctttattttt cagatcgaca gtatgcccgc ggtgatccgc gcggtgacgt
     6061 acattattcc cgctcgttat ttcgtcagca ccctgcaaag cctgttcctc gccgggaata
     6121 ttccagtggt gctggtggta aacgtgctgt ttttgatcgc ttcggcggtg atgtttatcg
     6181 gcctgacgtg gctgaaaacc aaacgtcggc tggattaggg agaagagcat gtttcatcgc
     6241 ttatggacgt taatccgcaa agagttgcag tcgttgctgc gcgaaccaca aacccgcgcg
     6301 attctgattt tacccgtgct aattcaggtg atcctgttcc cgttcgccgc cacgctggaa
     6361 gtgactaacg ccaccatcgc catctacgat gaagataacg gcgagcattc ggtggaactt
     6421 actcaacgtt ttgcccgcgc cagcgccttt acacatgtgc tgctgctgaa aagcccacag
     6481 gagatccgcc ccaccatcga cacacaaaag gcgttactac tggtgcgttt cccggctgac
     6541 ttctcgcgca aactggatac ctaccagacc gtgccgttgc agttgatcct cgacgggcgt
     6601 aactccaaca gtgcgcaaat tgccgccaac tacctgcaac agatcgtcaa aaattatcag
     6661 caggaactgc tggaaggaaa accgaaaccc aacaacagcg agctggtggt acgcaactgg
     6721 tataacccga atctcgacta caaatggttt gtggtgccgt cgctgatcgc catgatcacc
     6781 actatcggcg taatgatcgt cacttcactt tccgtcgccc gcgaacgtga acaaggtacg
     6841 ctcgatcagc tactggtttc gccgctcacc acctggcaga tcttcatcgg caaagccgta
     6901 ccggcgttaa ttgtcgccac cttccaggcc accattgtgc tggcgattgg tatctgggcg
     6961 tatcaaatcc ccttcgccgg atcgctggcg ctgttctact ttacgatggt gatttatggt
     7021 ttatcgctgg tgggattcgg tctgttgatt tcatcactct gctcgacaca acagcaggcg
     7081 tttatcggcg tgtttgtttt tatgatgccc gccattctcc tttccggtta cgtttctccg
     7141 gtggaaaaca tgccagtatg gctgcaaaac ctgacgtgga ttaaccctat tcgccacttt
     7201 acggacatta ccaagcagat ttatttgaag gatgcgagtc tggatattgt gtggaatagt
     7261 ttgtggccgc tactggtgat aacggccacg acagggtcag cggcgtacgc gatgtttaga
     7321 cgtaaggtga tgtaacttct tatctttcgc cagcaaagac actaccgccg ggccggcaag
     7381 gattgccagc cctgcaattg ccagcaaaaa gttgttttcc agaacgcggg acaacagcca
     7441 cagcacaatc agcgctgcgc caaaatacca ggtcgtggtc agctcttcca gcacgtctgc
     7501 cacctcacgc cagcgttgct cggggtggcg ctgaaacacc agcatcatca ctaccgtgac
     7561 acaatacagt gcgcataatc ccacgccgac gtgaaccaga gtcttactca tccagcccac
     7621 caccagcagc gccactacca gtaaatgcaa cataatctgc cagcaactta gacccgttgc
     7681 gacacgaaca cgttgttgcc acttcatggc ttctctcctg aagaatttat ctctacttac
     7741 tcagagtacc gaactttacg gaaaattccg taagaccgca cctttttgtg acgccgacta
     7801 cactatttca atgagataag aggcaaagac aggaaaatta tgcccgatca aacacaacaa
     7861 ttttcgttca aggtgctcac catcaatatt cacaaaggct ttaccgcgtt taaccgacgc
     7921 ttcattttgc cggaacttcg cgatgccgtg cgtaccgtca gcgccgatat tgtttgcctg
     7981 caggaagtga tgggcgcgca cgaagttcat ccgctgcatg tggaaaactg gcccgatacc
     8041 tcgcactacg agtttctcgc cgacactatg tggagcgatt ttgcctacgg tcgcaatgcc
     8101 gtatacccgg aagggcatca cggcaacgcc gtactgtcgc gttatcccat tgaacattat
     8161 gagaatcgcg atgtttcggt cgatggtgcg gaaaagcgcg gcgtgctcta ctgccgcatt
     8221 gtgccgccga tgaccggaaa agcgattcat gtgatgtgcg tacatctggg cctgcgtgag
     8281 gcgcaccgtc aggcgcagct tgcgatgctc gccgaatggg tgaatgagct accggacggc
     8341 gaaccggtat tggtggcggg tgatttcaat gactggcggc aaaaagctaa tcatccgtta
     8401 aaagtgcagg ccggactgga tgagattttt acccgcgccc acggacgccc ggcgcgcacg
     8461 tttccggtgc aatttcctct actacgactg gacaggatct acgtcaaaaa tgccagcgcc
     8521 agcgcgccaa ccgcgttgcc gctgcggaca tggcgacacc tttctgatca tgccccttta
     8581 agtgcggaga ttcatttatg aaatgtagct ggcgcgaagg caataagatc cagttgctgg
     8641 aaaacggcga gcaatattat cccgcggtgt ttaaggcgat tggcgaggca caagaacgca
     8701 tcattcttga aacgtttatc tggtttgagg atgacgtcgg caaacaactg catgcggcac
     8761 tactggcagc agcgcaacgc ggggttaaag cggaagtctt gctggatggc tacggttcgc
     8821 cggatctcag cgatgagttt gtcaatgaac tgacggcagc tggcgtggtg ttccgctact
     8881 acgatccccg ccctcgcctt tttggtatgc gcaccaatgt gtttcgccgg atgcatcgca
     8941 aaattgtggt gatcgatgcg cgtatcgcct ttattggcgg gctgaattat tccgccgagc
     9001 atatgtccag ctacggtcca gaggccaaac aggattacgc ggtgcgcctt gaagggccga
     9061 ttgtcgaaga tattctccag tttgagctgg aaaacctgcc tggacagagc gcggcacgac
     9121 gctggtggcg acgtcatcac aaagcggaag agaaccgcca gccgggagaa gcgcaggtat
     9181 tgctggtctg gcgcgataac gaagaacatc gcgatgatat tgaacgtcac tatctgaaaa
     9241 tgctcactca ggcgcgtcgg gaagtgatta ttgccaacgc ctacttcttc cccggctatc
     9301 gatttttaca cgccttgcgt aaagcggcac ggcgcggggt gcggatcaaa ctgatcattc
     9361 agggcgaacc ggatatgccg attgtcagag tcggtgcgcg cttgctgtat aactatctgg
     9421 ttaaaggcgg cgttcaggtg tttgagtacc gccgccgccc gctccacggc aaagtggcat
     9481 tgatggacga tcactgggcg acagtagggt ccagtaatct cgatccgctc agtttgtcac
     9541 tgaatctcga agcaaatgtc atcatccacg atcgtcattt taaccagacg ctgcgcgata
     9601 atctgaacgg cattattgcc gcagattgtc agcaggtcga tgagaccatg ctgccgaaac
     9661 gcacctggtg gaacctaacc aaaagcgtgc tggcgttcca ctttttacgc cacttcccgg
     9721 cgctggttgg ctggcttccg gcacacacgc cacgtctggc gcaggttgat ccccccgcac
     9781 aaccgacaat ggaaacgcag gatcgggtag aaactgaaaa cacgggggta aaaccctgat
     9841 gagtaaatca cacccgcgct ggcgcttagc aaagaagatc ctcacctggc tgttttttat
     9901 cgcggtgatt gtgttgctgg tggtctacgc caaaaaagtg gactgggaag aggtctggaa
     9961 ggtcatccgc gactacaatc gcgttgcgct gcttagtgcg gtcgggctgg tggtcgtcag
    10021 ctatctgatt tacggctgct atgacctgct cgcccgcttt tactgcggtc acaaactggc
    10081 gaagcgccag gtgatgctgg tgtcgtttat ctgctacgcc ttcaacctga cgctcagtac
    10141 ctgggtcggc ggcattggta tgcgctatcg tttgtactct cgtctggggt tgcccggtag
    10201 cactattacg cggattttct cgctcagtat taccaccaac tggctgggct atattttgct
    10261 ggcggggatt atctttaccg caggcgtggt ggagctaccg gatcactggt atgtcgatca
    10321 aaccacactg cgcattctcg gcattggctt actgatgatt atcgcggttt atttgtggtt
    10381 ttgcgctttc gcgaagcacc gccatatgac catcaaaggg caaaagctgg tgctgccctc
    10441 atggaaattc gccctcgccc agatgttgat ttccagtgtt aactggatgg tcatgggagc
    10501 gattatctgg ctgttacttg gtcaaagcgt gaactatttc tttgtactgg gcgtgttgct
    10561 ggttagtagt attgcaggcg tcatcgtgca tattccggcg gggattggtg tgctggaagc
    10621 ggtatttatc gcattactgg caggggagaa tacatccaaa ggcacaatta tcgccgccct
    10681 gctcgcttat cgcgtcctgt attactttat cccgctgctg ctggcgctga tctgttacct
    10741 gctactggaa agccaggcga agaagctacg ggcgaaaaat gaagcggcga tgtgaagttc
    10801 agttaagttc tttagtatgt gcatttacga ctaatgaaaa aaacgcgtat gcctttgcca
    10861 gacaagcgtt atagctatcg cggcaagtat cccaaagaag ccgataaaat agtaatagag
    10921 tgttaatgca cctataaaag cgcaacgaac cggactccac aactcttcac tatggcattt
    10981 ccccgccagt tcaaggagct tatttcgtcc atgcgagata atgcctgacc ataacacaat
    11041 cattaagcaa cttattaccc aaatgggccg ttcactcatt aatgccgcat tcacgatgat
    11101 taccagggcc aaccctgtca acgtcatcat aatgataaaa cggtgagaac caggatcaac
    11161 attaaataat aaaccggcca gcatactgat taaaaacatc gcgcccgcag tgctgaacac
    11221 gatgataatg gcatatacag gaactaaact tgcaacgaca atcgccacca aagagagtaa
    11281 aaacagatat attacgcctg cgatggcggc atttatcttg ttgatatcct tacttaagcc
    11341 atacatacaa agtaataata accctatggc gacatacatt ggtggtgaat catatttggt
    11401 tgccgtaatt tcgagatact tagcacccca ggccagccct gcggttgcca ccaaacctaa
    11461 agcggtaatg agccatatac cgtttaatat cctggtctcg tgctgaaaat ctaatttatt
    11521 gctgttttgc aagtatgatt ccattctttt agttgtcctt atagaaaaat attaatattc
    11581 atcatgaata aatttaaacc cacgcataca aaatactttt gtatttatgg atacggaaaa
    11641 cgcttccgct cggccataag gcatatttta tacctggtac ttgttaagtg tcgtttttag
    11701 taagaaaaaa aatcccggca ccaggccggg atcagcagga agaagagaat taacgtcgat
    11761 tgccaaaaat tcgcagcaac atcaggaaca ggttgatgaa gtccagatac aaggttaacg
    11821 cgccaagaat ggaatatttg cgcaggttcg acgagtcgcg ggtatctatc tgctcaccca
    11881 tatttttcag tttctgggtg tcatacgccg tcaatccgac aaagacaatc acgccgatgt
    11941 aggtaactgc ccacatcaat gcttcgcttt tcagccagaa gttgaccagc gatgccagca
    12001 caatgccgat taacgccata aacagcatat tgccaaagcc acttaaatcg cgcttcgtgg
    12061 tgtaaccgta caggctcatt gcgccgaaca tcccggcagt aacgacgaag gtactggcga
    12121 tagaagcagc ggtataaaca atgaaaatac tggaaagcgt aagacccgtc agcgccgaat
    12181 aaagcataaa gagcatcgtc gttacacctg cgctcagctt ttgaatcatc gctgataaca
    12241 caataaccaa tgctaattgc gcgatgatca gaccgattaa aaagacacgg ttagtgaaca
    12301 acagctccat cacggccgcg gaattagccg cataccaggc aacaaatgcg gtcagcaaca
    12361 agccaacggt catccagcca tagacttgcg ccatataagt ctgcaagcca gcccggggtt
    12421 gtacaattga atcagaacgt gggaatctgt ccatgacgat ctcctgaaga tataaggaat
    12481 atcttaagga tactgcaaaa tgatgaggct gtgcatcgac gcagcgtaaa cgcatgtact
    12541 gagcggtgaa attgccggac gcagcggtgc cttatccggc taacaaaaac taccagcgtt
    12601 ttgccgcctg ctgatcgctc tcccgagctt cgacccagcg gtcgccctcc ggcgtggctt
    12661 cgcgcttcca gaacggtgcg cgggttttga gataatccat aataaactgc ccggcttcaa
    12721 acgcactgct gcgatgcgca ctggtgacac cgacaaaaac gatttcatcg cccggccata
    12781 attccccgat gcggtgaatc accgtgacgc gccccagcgg ccagcggtta cgcgcttcat
    12841 cgacaatttc tgccagtgct ttttcagtca tccccggata gtgttcgagg gttaatgctt
    12901 tgacgctgtc gcccaggtta tggttgcgca ccttaccggt aaaggtgact accgcaccgt
    12961 cttcgtcacg ctccgccagc cacgggtact cttctcctac gctgaacggc tgcggaccaa
    13021 caacaatttt ggtttctgcc atcttaacct ccggttaccg gcgggaagaa agctacttcg
    13081 tcgccgtcag tcagcggatg gtcaaaactc accagcgtct ggttgacggc agccagtaat
    13141 ttgccatctt ccagcgccag cgcccagcga tcgctctgcg cagccaggtg ctggcgtaac
    13201 gcttcaacgg ttgggaaatc cgcagccact tcggttgcat ctgttcctac caactcgcgc
    13261 acctgggcga aaaaaagaac tttaatcatc cgcttccacc ttaaagtcac ccgacttgcc
    13321 gccgctcttc gccagcaaac gtaccggacc aatcaccata tctttttgca ccgctttgca
    13381 catgtcataa atggtcagcg ccgccacgga ggccgcggtt aacgcttcca tttcgacacc
    13441 ggttttcccg gtcaggcggc ataaggtttc tatacgcacc cgattgtgct ccggctcggc
    13501 ctgtaaattg acttcaactt tgctgagcat cagcggatga cacagcggga tcagatccca
    13561 ggtgcgtttt gccgcctgaa taccggcaat acgcgcagtg gcaaatacgt cgcctttgtg
    13621 gtggcgacca tcaataatca tcgccagcgt ctcgctgcgc atggtgacaa aggcttccgc
    13681 ccgcgcttca cgcacggttt ccgctttggc ggagacatcc accatgtgcg cttcgccagc
    13741 ggcgttgata tgggtcagtt gcgacatact tatttcttca aatgtggatg gaaattacac
    13801 ggacgcgtac gggcatccag ctgcggcgcg atgatatttt cccatgcggt acggcacgct
    13861 ttggtcgaac ccggcatggc gaaaatcagc gttttgttag cgacgcccgc taccgcacga
    13921 gattgcaacg tggaagtgcc aatctcttca aacgacaaca tacggaacac ttcaccaaaa
    13981 ccttcaactt cacggtcgaa caacggcagc aatgcttcgg gagcctgatc accttccgtc
    14041 aggccagtcc cccccgtaat caacaccact tgtacatcgt cgctggcgat ccacgcagat
    14101 acctgagcgc gaatagcgta gcggttttct ttcacaatgg ctttatcgac aacgtgatgg
    14161 cccgcttctt gcgccgaatc gcgcaaatag tgaccggagg tatcgtcttc ttcaccgcga
    14221 cgattagaaa ccgtaagaat agcaatacgg gtcgggataa attcagtgct tacctgactc
    14281 atctgatctc tccttttgac gttttagccg ccaatgtacg ataagttttg cgtaataccg
    14341 gtgttgtttt gatgcaggaa atgggtctgt ttcttctccc gcagcgccgc tgaaatacgc
    14401 gcttccagcg cctgttgctg ggtatcgtct tccagcagat cgcgcaggtt aacgccgcct
    14461 tcaccaaaca ggcagagatg gagtttacca atggaggaaa cgcgcagacg gttgcaagtg
    14521 gcgcagaagt ctttttcata cggcatgata aggccaatct ctccggcgta atccggatga
    14581 caaaagactt gcgcgggacc gtcgctgcgt tgacgtaatt ggtggatcca gccgcgacgc
    14641 agtagctcgt cacgcagaac ctgaccagag atgtgatgct tacggaagag ctcgctgccc
    14701 tcgcccgttt ccatcagttc gatgaaacgc agctggatag ggcgatgctg gatccagttc
    14761 agaaaggtgt cgagctggtg atgattaaca tcacgcatca gcacggtatt gactttgacc
    14821 ttctcaaaac cggcctcaaa tgcagcatca atccctgcca tcacctggtt gaatttatcc
    14881 tgcccggtaa tagcgtgaaa ctggcgggcg tccagactat caacgctgac gttgatgcca
    14941 gtaagtccag catcgcgcca gttcgccaca tcgcgttcca gacggtaacc attggtggtg
    15001 accgctatct ggcgaatagc gtcgttttct cgcacagcgg cgatgatatc ggtaaagtcg
    15061 cggcgtaaag acggctcacc gcctgtcagg cgcacttttt cggtgcccag actggcgaag
    15121 gcgcgcgtaa cccggcgaat ttcatcgacg gtcagaaagc ctttattggt gacgccgctc
    15181 ggtttgtagc catccggcag gcagtaggtg caacgaaagt tacacacatc ggtaatcgac
    15241 aggcgcaagt agtaaaactt acgcgcaaat gcatcagtca gttgtgaagc catgtacacc
    15301 tttccagata cgggaggcga agtcatttct tccttcgccc tggtggcaat ctttacgagc
    15361 attgtcacgg ccaaagcacc atatcagttg acccaggtgc agaggctaga gtgtttagtg
    15421 gttaagccga tactagcgtg taaatgagat ttttaccatc cacactttcg ctatgtaatc
    15481 atgtatatag cgtcatgatc gtgctatttc gctacagaat aagggaaaat ggcggtttta
    15541 ctttgatata catcatcagg gaggcatagt gtgcatcgtc ctttagaggt agaagaaaat
    15601 agtttgtatc ctcaatattg gcaggttaat tgctgtttcc ccgcaatttg cgctactgta
    15661 gcgcggataa tctgactcca ggaaactata tgcgcaatcg tacgctggct gatcttgatc
    15721 gtgtcgttgc tctcggcgga gggcatggac tgggacgcgt tctctcatca ctttcgtctt
    15781 tgggttctcg tttaacgggt atcgtcacca ccacggataa tggtggctcg acggggcgta
    15841 ttcgccgttc agaaggcggc attgcctggg gcgatatgcg caactgcctt aaccagctga
    15901 taactgaacc cagcgtcgct tccgcaatgt ttgaatatcg tttcggcggt aatggcgaac
    15961 tttccgggca caaccttgga aacctgatgt tgaaggcact ggatcacctt agcgtgcgac
    16021 ctcttgaagc gatcaatctg attcgaaatc tgctgaaagt ggacgcacat ttgattccga
    16081 tgtcggagca tcctgttgat ctgatggcga ttgacgatca ggggcatgaa gtttacggcg
    16141 aggtcaatat cgaccagtta actacgccga ttcaagagtt attgttaacg cctaatgtac
    16201 ccgcaacgcg tgaggcggtt cacgctatca atgaagcgga tctcatcatt attgggcctg
    16261 gcagttttta taccagcctg atgcccattc tgctgctgaa ggaaatcgcg caggcattac
    16321 gccgcacgcc agcgccgatg gtctatatcg gcaatctggg gcgtgagttg agtttacctg
    16381 cggccaattt gaagctggaa agcaagctgg caattatgga gcagtatgtt ggtaaaaagg
    16441 tcattgatgc ggtcatcgtc gggccaaaag tggatgtctc ggcggtgaaa gagcggattg
    16501 tgattcagga ggtgctggag gccagcgata ttccgtatcg tcatgatcgc cagttgttac
    16561 atagcgcact ggaaaaggcg ttacaggctt taggttaacg gaaatccgaa ggaaaattcc
    16621 ggcttcctat tgaagacaaa gtgcgcgttg tttatgccgg atgcggcgtg aacgccttat
    16681 ccggcctaca aaccgcgcta attcaatgta ttgcagaatc atgtaggcct gataagcgta
    16741 gcacatcagg caattttgct tttgtcatct gtcttcttcg ttatcctgtc acgaagccgc
    16801 gataaacaat tcacgcagct gatgcaactg gtcacgaatt tgtgccgctt cttcgaactc
    16861 cagattctgc gcgtgttgca tcattaaccc ttccagctca tggattttct gttgcaacgc
    16921 tttaggcgac atatccatcg gcacattatc cggctcaaca atcgggcgcg attttcctct
    16981 gcccttcgct ttggttttgg cgatgttctg ccccagcgcc aggatatcga ccactttctt
    17041 gttcaagcct tgcggcgtaa tgccgtgttc ctcgttgtac ttctgctgtt tctcgcggcg
    17101 acgttcggtt tcgccaatcg ctttcgccat tgatggggtg atcttatcgc cgtagagaat
    17161 cgctttaccg ttaacgttac gtgccgcacg accaatggtc tggatcaacg aacgttcgga
    17221 acgcaggaag ccttctttgt cagcgtcgag gatcgccacc agcgaaacct ccggcatatc
    17281 cagaccttcg cgcagtaagt tgatccccac cagcacgtcg aactcaccca gacgcaagtc
    17341 gcggataatc tccatacgtt cgacggtgtc gatatccgag tgaagataac gcacgcgctc
    17401 gccatgttct tcgagatatt cggtgagatc ttccgccatc cgcttggtca gtgtggtgac
    17461 cagtacgcgt tcgttaattg ccgctcgctg acgaatctcc gaaagaagat catcaacctg
    17521 tgtcgccact ggccgcactt cgataatcgg atcaagcaat ccggttggac gcaccacctg
    17581 atccaccaca tcgccgccgg atttttccag ctcataatta cctggcgtcg ccgaaacata
    17641 gatggtttgc ggcgctaatg cttcgaactc ttcaaatttc agcggacggt tatccagcgc
    17701 tgatggcagg cggaagccgt actccaccag tgtctcttta cgcgcccggt caccgcgata
    17761 catgccgcca atttgtggaa tggtgacgtg agattcatcg accaccagca gcccatcggc
    17821 aggcaggtaa tcaaacagcg tcggcggtgg ctcacccggt ccacgaccag agaggaagcg
    17881 cgagtagttt tcaatccccg aacagtagcc cagctcgttc atcatctcca gatcaaactg
    17941 ggtacgctgg gtcagccgct gctcttccag cagtttgttg ttttccaaca gcactttgcg
    18001 tctggcggcc agctcttctt tgatctcctc catcgcctgt acgatgcgct cgcgcggtgt
    18061 gacgtagtgc gttttcgggt agatggtaaa acgtggaata gtggaaacaa tctgcccggt
    18121 cagcgggtca aataacgaca atcgttccac ttcctcgtca aacagttcca cgcgaagtgc
    18181 aatgtcatcc gattctgccg ggaatatatc aatcacctcg ccacgaacgc ggaaagtacc
    18241 gcgctggaat gcctgatcat tacgagcgta ttgcagctcc gccagtcggc gcagaatcgc
    18301 gcgctgatcg ataatcatac cgaccgtgag atggagcatc atcttgagat ataaatcagg
    18361 atcgcccaga ccataaatcg cggaaacaga cgccaccaca accacatcac gtcgctccag
    18421 catcgctttg gtggcggaca aacgcatctg ttcgatatgt tcgtttaccg aggcatcttt
    18481 ctcaatgaag gtgtcagaac tcggtacata ggcttccggc tgatagtagt cgtagtagga
    18541 gacgaaatat tccaccgcgt tttccgggaa gaactctttc atttcgccat acagctgggc
    18601 cgccagcgtt ttgttgggcg caagtaccat ggtagggcgc tgaaggtcag caatgacatt
    18661 ggcaatggtg aaggttttcc cggagccagt cacgccaagt aacgtctggt gcgccaggcc
    18721 atcttccagc ccctcttcga gacgtcgaat cgcctctggc tgatcgccag aaggtttaaa
    18781 agcggaattc agtttgaacg gtttactcat gagtctctac ctgaaggagt tgggcgggca
    18841 ggtatgtaat tttactcgtc gtacttaatt atgccaacaa attatactgg ataaaaaaac
    18901 agttcatcac cataatattt ctgatacagc gtaaactccg ctgtcaatca tgagcaaaat
    18961 ttactctgtg gcgagataaa actccggcct taccgggtta tccccaaagc aacggctttt
    19021 ttaacatttg tcaagatgag tggtgacagt tttatggcaa gagatgcctc gttagtgact
    19081 gccattgcaa ttatctaacc agttaaaaaa taaaagaaat ttctttgagc cgtctttaac
    19141 gccaattcgc gtgcaagccg cgtattctct cgcttgcctc gtgttttcta actctaatac
    19201 acatggttat ccacaggaat agtggataac tgtctgcagc ccctatcccc cgccgcctgg
    19261 gcaattccca cattcgccgc atgatgcgga agtaaaattt ttttcgtgat tattttagaa
    19321 tttaattggt taaattgcag tcaatcgaag acgcgatctc gctcgcaatt taaccaaaca
    19381 caggatggct acaacaaggc aaggtttatg tactttccgg ttgccgcatt ttctggattt
    19441 tctgcaagcc tggggatctc tcccagcagc ggcgcgggaa tcatgcgggt gagcgtggtc
    19501 atatattcag cgtgacgttt tcccggaggc gtgacatcgt tcgccaccca acccgccaga
    19561 gtcagcccgg cgtgttgtat cgcctgtgcc gtcaacatcg cgtgattaat acagccaagt
    19621 ttcacgccaa ccaccagtat taccggcagt tgttcctgtg ttacccaatc cgcaaaagtg
    19681 aaagtgtcag aaagcggcgt aaaccagccg ccagcgcctt ccactaacac ccagtcagcc
    19741 tgttgttcaa gcgcgcgtaa tccggagctc attaccgatg attctatcga cctgccctct
    19801 tgcgcgctga tgatgtgcgg tgaggtgggt tctgcgaagg tgtaaggatt taccgttgcg
    19861 taatccagct gcaggctgct gttgcgctgt aacgccagcg cgtcactatt acgtaaaccc
    19921 tccggggtct tttcgctgcc agaggcgacc ggtttataac ctgccgtccg atagcctgct
    19981 gcctttgcgg cttgtaaaag tgcacaactg gcgacagttt tccccacttc ggtatccgtt
    20041 ccggtgacaa aataacgttt actcacgaac aatcactccc aaaaaaagat gatacgtcag
    20101 aggatatcgc ccctgttgtt gcggccaggc cagttgcaat cgctgcaact gtgaacgcgt
    20161 taatattcgc tggtcgcgcc cttcatgaag atgcgtggca ccgatgcctt tcagcgaacg
    20221 catggcactg agcgcatcat caaaccacag cgtgatgggc tgaatatgat gtcgataatg
    20281 cacgccgttc agtgactgtt cgatttcatc tggcggtaaa aagcgattag catgcgggcg
    20341 ctcgtccacc gcctgccacg cctgatgcag ttcgggtaac gatccctgca ccagcgtggt
    20401 aaacgcgacc acgcctccgg ggcgcaccac ccgatacagc tcgcggagtg ccgtagataa
    20461 attaccgcac cactgcaccg cgagattgct ccatacaaga tcgaacgtcg cagtcgctaa
    20521 cggcagggat tcgatatctc ccgccagata atgatctgcg gcatctttat ggcgggcttg
    20581 cgcaagcatt ggcggcgaga gatctaacgc cgtcacctgc gcgtgacgct ctcgccagtg
    20641 gcggctcatc cagcctggcc cacaccccgc gtccagtacg tgggtgtatt tacgctgtgg
    20701 gagcattgcc agtaagacgt cagcactctg gcgctgtaac tctgcgtgtt gctcatagtg
    20761 cgcggctgcc cgaccaaatg ccgctgcaat ggcttgttta ttaaccgttg ccatgcagca
    20821 cctccagcag acggtcgata tcctgcattt catgcgcgga ggttagtgtt aagcgcagtc
    20881 gcgcagtacc agcgggtacg gttggcgggc gaatcgccgt gacccagcag ccttgctggc
    20941 gcagtttttc tgccagttgt aacgcacggc tgttatcacc gacgatcaat ggctggatgg
    21001 cgctgcatga atcagcaagc gtaaacggca aatcctgtac tccggcacga aaacgcgtaa
    21061 tgagtgccgc cagtttttcg cgccgtgcat caccctcatc actgcgaatg accgccagtg
    21121 acgcacgtaa tgcctgcgcc tgagcgggcg gcatactggt gctgtagata agatggcggg
    21181 cgaattgcag cagataatcc gccaccgtac tggagcaaag caccgcagcc ccgctgacgc
    21241 caaatccttt gccaaaagtc actaccagca attctggttt taccttttgc aaccagcagg
    21301 tgccgcgccc ctgctccccg ataacgcccg tgccgtgggc atcatcgacc atcaaccagc
    21361 cattgtgctg ttgcgttacc tgctggattt ccgccagtgg cgcactatcg ccgtccatgc
    21421 tgaacacgcc ttctgtcacc accagttgct gccccgaaca gggggaagca agcagtcgcg
    21481 ccagatgagc gacatcgtta tggataaaac ggcgaagctg cgacgggctt aaactggcag
    21541 cctccagcaa tgaggcatgg ctaagccggt cggcaacaat acggtcctct ttcgccatca
    21601 tcgcggcaat gactgcctga ttcgcggcaa aaccagagat aaacagcagc gcccgcgaat
    21661 agccaagcca ctcggccagt tcttcttcca gcgcctgatg cgccacgcta taaccgctga
    21721 catgcccgga accgccgcta cctacaccaa acttttctgc gccctgctgc caggcgcgga
    21781 taatttgcgg atgatggctt aaacctaaat aatcgttact ggaaaagttc agatactggc
    21841 gatcatccgc caccagccag cgtccggctc cttgcgtcac cggataacgg cgacgcaggg
    21901 catcggcagc acgccgcgca tcgagcgccg cgttgatttt ctcctgccag ctcatagtgc
    21961 tgccgcgttg taatattcgt cagtatccgg ggtcatcagt gcctgctcca gacgctgctg
    22021 ttgttcgtta tcccctgcca gcacggcagt ttgctgcgga tttaacccca gtttgcgaaa
    22081 cagttgcagg tctttatctt cttccggatt cggcgtagtc agcagtttgc agccgtagaa
    22141 aatcgagttt gcgccagcca taaagcacat cgcctgagtc tgttcgttca tctgctcgcg
    22201 tccggcagaa aggcgtacgt aagaggtcgg catcatgatc cgcgctacgg caatggtgcg
    22261 aataaaatca aaggcatcaa cgtcatcgtt atcggcaagc ggcgtgcctt tcaccttcac
    22321 cagcatgttg attggcacgc tttccggcgg cgtcggcagg tttgccagtt gcagtaataa
    22381 tccggcgcga tcttttaccg tttcgcctaa acccacaatg ccacctgagc agactttgat
    22441 cccggcatcg cgcacttttt ccagcgtatc aaggcgttcc tgataagtac gtgtggtgat
    22501 gatattgccg taaaactccg gcgaggtgtc gaggttatgg ttgtagtaat ccagcccggc
    22561 attcgcaagg cgctgcgctt gagattcact caacgtgccc agcgtcatac acgcctccag
    22621 ccccatcgct tttaccccct gcaccatttg ttccaggtac ggcatatcgc gttcgtgggg
    22681 attcttccac gccgcgccca tacagaaacg cgtcgatcct gccgctttcg ctttgcgcgc
    22741 cgactccagc acctgttcaa cttccatcaa cctctcggct tccagcccgg ttttgtagcg
    22801 cgagctttgc gggcagtatt tgcaatcttc cggacaagct ccggtcttaa tcgacagcaa
    22861 cgtgctgacc tgcacctgac gaggatcgaa atgctggcga tggacctgct gcgcttcaaa
    22921 cagcagatcc agcaacggtt tttcaaataa ttctgtgact tgcgacaatg tccagcgtgg
    22981 gcggtgagcc atggggcttc tccaaaacgt gttttttgtt gttaattcgg tgtagacttg
    23041 taaacctaaa tcttttcaat ttggtttaca agtcgattat gacaacggac gatcttgcct
    23101 ttgaccaacg ccatatctgg cacccataca catccatgac ctccccgtta ccggtttatc
    23161 cggtggcaag cgccgaaggt tgcgagctaa ttttgtctga tggcagacgc ctggttgacg
    23221 gtatgtcgtc ctggtgggcg gcgatccacg gctacaatca cccgcagctt aatgcggcga
    23281 tgaagacgca aattgatgcc atgtcgcatg tgatgtttgg cggtatcact catgcgccag
    23341 ccattgagct gtgccgcaaa ctggtggcga tgacgccgca accgctggag tgcgtttttc
    23401 tcgcggactc cggttccgta gcggtggaag tggcgatgaa aatggcgttg cagtactggc
    23461 aagccaaagg cgaagcgcgc cagcgttttc tgaccttccg caatggttat catggcgata
    23521 cctttggcgc gatgtcggtg tgcgatccgg acaactcaat gcacagcctg tggaaaggct
    23581 atctgccaga aaacttgttt gctcccgccc cgcaaagccg catggatggt gaatgggatg
    23641 agcgcgatat ggtgggcttt gcccgcctga tggcggcgca tcgtcatgaa atcgcagcgg
    23701 tgatcattga gccgattgtc cagggcgcag gcgggatgcg catgtaccat ccggaatggt
    23761 taaaacgaat ccgcaaaatg tgcgatcgcg aaggtatctt gctgattgcc gacgagatcg
    23821 ccaccggatt tggtcgtacc ggcaaactgt ttgcctgtga atatgcagaa atcgcgccgg
    23881 acattttgtg cctcggtaaa gccttaaccg gcggcacaat gaccctttcc gccacactca
    23941 ccacgcgcga ggttgcagaa accatcagta acggcgaagc cggctgcttt atgcatgggc
    24001 caacttttat gggcaatccg ctggcctgcg cagcagcaaa cgccagcctg gcgattctcg
    24061 aatctggcga ctggcagcat caggtagcgg ctattgaagc gcagctgcgc gagcaacttg
    24121 cccccgcccg tgatgccgaa atggttgccg atgtgcgcgt actgggggcc attggcgtgg
    24181 tcgaaaccac tcgcccggtg aatatggcgg cgctgcaaaa attctttgtc gaacagggtg
    24241 tctggatccg gccttttggc aaactgattt acctgatgcc gccctatatt attcacccgc
    24301 aacagttgca gcgtctgacc gcaggggtta atcgtgcggt acaggatgaa acattttttt
    24361 gccaataacg ggaagtcagc gtgagggttt ctggctacac tttctgcaaa ctagaaagga
    24421 gggtttatga aactcatcag taacgatctg cgcgatggcg ataagttgcc gcatcgtcat
    24481 gtctttaacg gcatgggtta cgatggcgat aatatttcac cgcatctggc gtgggatgat
    24541 gttcctgcgg gaacgaaaag ttttgttgtc acctgctacg acccggatgc gccaaccggc
    24601 tccggctggt ggcactgggt agttgttaat ttacccgctg atacccgcgt attaccgcaa
    24661 gggtttggct ctggtctggt agcaatgcca gacggcgttt tgcagacgcg taccgatttt
    24721 ggtaaaaccg gatacgatgg cgcagcgccg ccgaaaggcg aaacccatcg ctacattttt
    24781 accgttcacg cgctggatgt agagcgtatt gatgtcgatg aaggagccag cggcgcgatg
    24841 gtcgggttta acgttcattt ccactctctg gcaagcgcat cgattaccgc gatgttcagt
    24901 taattactct gccagatggc gcaatgctat ctggtatcac ttaaaggtat taaaaacaac
    24961 tttttgtctt tttaccttcc cgtttcgctc aagttagtat aaaaaagcag gcttcaacgg
    25021 attcattttt ctatttcata gcccggagca acctgtgaac acattttcag tttcccgtct
    25081 ggcgctggca ttggcttttg gcgtgacgct gaccgcctgt agctcaacac cacccgatca
    25141 acgtccttct gatcaaactg cgcctggtac ctcttctcgc ccgattctgt cggcaaaaga
    25201 agcgcagaat ttcgatgctc aacactattt tgcatccctg acaccaggtg ctgcagcgtg
    25261 gaatccgtcc ccgataaccc tgcctgcgca acctgacttt gttgtcggcc cggcgggtac
    25321 tcaaggtgta acgcatacca cgattcaggc ggcggtagat gcggcaatta tcaagcgcac
    25381 caacaagcgc cagtatattg ccgtgatgcc tggtgagtat cagggaacgg tgtatgtccc
    25441 tgctgctcca ggtggaatta ctctgtacgg tacaggtgaa aaaccgattg atgtgaagat
    25501 tgggctttcc cttgatgggg gcatgagtcc tgccgactgg cgtcacgacg tcaacccgcg
    25561 cggcaaatat atgccaggta aaccagcgtg gtatatgtac gatagctgcc agagcaaacg
    25621 cagcgacagt atcggtgttc tgtgttctgc ggtcttctgg tcacaaaaca atggcctgca
    25681 actgcaaaac ctgaccatcg aaaacacgct gggtgatagc gtagatgcgg gtaaccatcc
    25741 ggcggtggca ctgcgtactg acggtgacca ggtacagatt aacaacgtta acattctcgg
    25801 tcgtcagaac accttctttg tcaccaacag cggtgtgcag aaccgtctgg aaacgaatcg
    25861 tcagccgcgt acgctggtga ccaacagcta cattgaaggg gatgtggata tcgtttctgg
    25921 tcgcggcgca gtggtgttcg ataacaccga attccgcgtg gtgaactcac gtactcagca
    25981 agaagcgtat gtgtttgcac cggctacgct gtccaacatt tactacggtt tcctcgccgt
    26041 aaacagccgt ttcaatgctt ccggtgatgg cgtggcacaa ctgggccgct cgctggatgt
    26101 tgatgccaat accaacggtc aggtggtgat ccgtgatagc gccatcaacg aaggttttaa
    26161 cacggctaaa ccgtggggcg atgcggtgat ctctaatcgt ccgtttgcgg gtaacaccgg
    26221 cagcgttgac gatagcgacg aaatacagcg caatctgaat gacactaact acaaccgcat
    26281 gtgggaatac aataaccgcg gcgtgggtag caaagtggtt gcagaggcga agaagtaaga
    26341 gctattaact atttgccgga tgcggcgtaa acgccttatc cggcctacgg ttcgatgcga
    26401 tttgtaggtc ggataagatg cgccagcatc gcatccgaca ataagtgccg aatgcgacct
    26461 acattcacat ggcgcttttt acatctgacg gtttttattg aagttaatca aactacccgc
    26521 cttgataatc tcgcgctctt cagcagtcag actttccata tagagcgtaa tttccgttac
    26581 cggcgcatct tcatggatca cataaccttt aaacgtcgta cccggattat ccagcgccgc
    26641 tttaatgcca gggatgtaaa tgtaatcccc cacttcaaag gttggcactt ccgccatctg
    26701 cagaggtaac atcccccagt tgatgacgtt agaacgatag cgtttagtcg cgtactcctc
    26761 ggcaatattc gccagaccgc caatcacacg ctgacaactc gccgcctgtt cacgcgcaga
    26821 accatcgcct ggtttcaccg catagaccat gctgccaatt tcagtttgca gcggatcaat
    26881 atgctcctga ccagcaatct gcttaatgcg cgcaaacacc tctgtcagct cgctgacatt
    26941 ccccgccaga cgctgatttt ccagctcagc agtcgcttta cttctgccaa cataacctgg
    27001 atcgcggcgt gacagggtaa actccgccag accaatcgga tttgaacgat aagaagaggt
    27061 ttcaccggaa gggatcagtt cgtcggtggt ggtcacttcg tcgaggatct tcgagcacac
    27121 tttgaggacg atattgtcag tcagcgcacc taattccggc cagtctttaa tgttcggtcc
    27181 gtaaatcagc ggttgctgag ttgccccttt aacaaagccc tgataaacac ggtttttata
    27241 cggcgttaca tcgaaggcgt actccggcac gttgtcccag caatcgagtt cgctggcaga
    27301 agttaaatag ccaccgtttg ccgcagtcgc agcgatagaa cgagcgtcca tcaacgccac
    27361 cgctgacatc tgcccattgg ctggcttaga accttcgcgg ttcgggaagt tacgcgtggt
    27421 gtggcgaata ctcaaaccgt tgttgattgg cgtatcgccc gcgccaaagc atgggccgca
    27481 gaacgcggtt ctgatgattg cgcctgcgcc aatcaaatct gctaccacac cttttttggc
    27541 taaatccata aatactggct gtgatgacgg gtaaaccgct aacgagaagg tgtcattgcc
    27601 acaggattga ccgcgcagtg cattcgccgc cgcgatgacg ttttcgtagt taccgccaga
    27661 acagcccgcg ataatcccct gctgcacttt caggcgacca ttttccactt tgtccagcag
    27721 cgagagtttg gctttaccgt gcgccacgcg ttcggactca atttcaatct cacgcagaat
    27781 gtcggtcagg ttctggttca gtgtgtcgat ttcatacacg ttgctcgggt ggaacggcag
    27841 cgcaatcatt ggtttgatgg cgcttaaatc aacgctgatg cagccatcgt agtacgccat
    27901 cggttgaggg ttaagctggc agtaatcctg gccgcgaccg tgcagcgcca gccagttatg
    27961 gacttcttca tcggtttgcc agacagaact taaacaggtc gtttcagtgg tcataacgtc
    28021 aacgctgtta cggaaatcgg tagagagcgc agcaacaccg ggacctacga attccatcac
    28081 tttgtttttg acgtagccgt ttttgaacac ggcaccgatg atagccagcg ccacatcctg
    28141 cggccccaca tacggcgctg gttttccggt cagatgcacc gcaacaactc ccggatagtc
    28201 gatatcccag gtgtcattaa gcaattgttt taccaactca ccgccgccct caccgactgc
    28261 cattgtccct aatgcaccgt aacgggtgtg gctgtctgac ccgaggatca ttttgccgcc
    28321 gcctgccatc atctcacgca tatattgatg gatgaccgca atatgcggag gcacaaaaat
    28381 accgccataa cgctgggccg ccgataaacc aaaaacatgg tcatcaccgt taatagtacc
    28441 gccgacggcg cagagtgagt tatggcagtt ggtcagcaca tacggcagcg ggaaacgttc
    28501 cattccggac gctttagcgg tctgtacaat accaacaaag gtaatatcgt gagaggctaa
    28561 tgaatcaaac ttaattttaa gtttatccat atttccggac gtattatgag aagagagaat
    28621 agaccaggca atagtgcctt tttttgcttc ttctttttta atttcgccgg tgaaatgttc
    28681 ttcggcaatt atttcgttat tactggcgag aaacacgcct ttttcagata acttgatcat
    28741 caaatgctcc agatatctgc gcaccgtgaa gtgcgcagaa agataataaa tggatatact
    28801 ctaaataatt cgagttgcag gaaggcgaca agcgagtgaa tccccaggag cttacataag
    28861 taagtgactg gggtgagcga acgcagacgc agcacatgca acttgaagta tgacgagtat
    28921 atttacagca tgttccagcc gatcagcata ttccaccacc acacgccgag ggtgatatgc
    28981 accaggaagg ttaatatcgt cagtaccgca ccgaccaacc accaggattt aatatcgttg
    29041 taacccacgc caaagatgac cggacctgcc gcgccgccat agtgagtaac catgccgcca
    29101 taggagttgg agaacaacag tgccagcgcg gttaacatta acggtgcgcc ggagacgttc
    29161 gccagcatgg caaatacagg taacatagca acgatatagg cactaccgga agcgaagaaa
    29221 taacgcacga tgatgctgag gaaaataata acgaagaaag caacgttacc gtgaccatca
    29281 aatgccaggt tatttttaaa gacttcagct aaccattcga agaatttaac tttcgataat
    29341 aaggagctta agccgataat accgccgtac caaattaaag tattccagcc gcctttattt
    29401 ttaaccacgt cttcccaggt aacaataccc agcagcagca tggttgccat aacaacgatt
    29461 gccacggtgg attcatcaac ccccagagac ttactgaaaa tccagcccag cagcgccagc
    29521 acaaagacac cgagcagcat tttttcgcgg attttcatcg gtcccagttc ggcaaggccc
    29581 gctttagcga tggttttgtt atccaccttc ttaatttctg gcggatacat ggtgtaaatc
    29641 accagcggag taaccagcag cataatgatg cccggcaatc cggcggccag cgcccatccc
    29701 ccccagctaa tttgcaggtg cagaatgtcg ttgatcattt tcagcgccag aatgttcccc
    29761 gccattgcgg taaagaacat atagctggtg gttttggtga ccatgtaaat ggacatcatc
    29821 aggtaatgtc cgacacgacg cggacttttt tccggttcgg accccaaagc caccgccacg
    29881 ctgttgatga tcggtaacac aatgccgccc gcacgcgcgg tgttagacgg tgttgccgga
    29941 gccagtacca gatcgaggaa aaccgtaacg taacccagac ccagcgtggt gttaccgatt
    30001 ttaccaatca gcagataggc aatacgttta cctaaaccgg tggtcacaaa tgcggcgctt
    30061 aaggtaaacg ccgagaacac cagccaggta gtacctgaag agtaaccgct taatacggcg
    30121 gtggttttaa acgcaccgtc ggataagtta ccgaccacca ccatggaggc agcaacggca
    30181 attaacagta cgacaggttc cgggaaaggc ttgataacca gccccacaat ggccgctagg
    30241 taaataccaa aaagcaccca cgccagttcg cttaatcctg ccggagctgg cataaagcca
    30301 ataatacatg ggatcgctaa tatcagaatt agcttccata acgatttctt attcatttaa
    30361 aatcctgttc tttctgtatg actcatacag aagtccttta ttgattgtcc ttatcctgac
    30421 aacgaatctt ttcagggaag ataaacttca ccggaaaata tttttctggt cgtccgaata
    30481 acagatgcgc gtaacgtcgt ggcatcctga ccttcattac ttaaatgaac gtcgagcgca
    30541 ccactggggt gttcaatatt gatattgcca tatcctacag aagggatgat ttgtcgggtg
    30601 acggtgcctt ccaatgcaca actactggaa atagcaatag caccggttat cgccaacgcg
    30661 cgatggcaag aatgcggcat aaaataacgc acattaattg ccccgccttt ctgcgctgga
    30721 gaaataagca caggtttagg gataaccata ttactgacat cacctaagcc cattgcttta
    30781 cccgcttgta gacggataga ttcaatgcgg gctaataatg ctttgtcggc atccagttcc
    30841 gccggtaatt catagcctgt ttttcccaga tattcagccg gaataatgac gactggcatc
    30901 gccatatcga tacaggtcac cgggacatcg tcaaaataat caatctgatt atcagtcggg
    30961 aaaacttttc cggttttggt tccagcggca ttcaggaaag tgagcgcaac cggtgcggca
    31021 gtacccggta cgccgtcaat tctggcacta ccctcgtact cgacaacacc atttggcgtt
    31081 tgcacatcag cttcgatgaa cgtacccgta ttgacgttgc ggatacgtac gcgggtaact
    31141 ggcgaagtcg ctgcaatcaa accattttca atggcgaatg ccccaacgcc agacagcata
    31201 ttgccgcagt taggcgtggt atcgacacgt tgctcatgga cgataacttg agcaaacaga
    31261 taatcaacat cagcacgcgg atcgctggaa cggctaataa tggcgacttt actggtcagt
    31321 ggattaccgc cgccaatacc gtcaatttcc agatcgttac cggaacccat aattgccatc
    31381 aatattttat cgcgctgcgt ttgatcttcg ggtaaatgtt ccgctaacag gaacgcgccc
    31441 ctcgaggttc cacctcgcat catcacgcag ggtatttttt tcataataat gccctcaatt
    31501 taaaaacata acttattgcg atatggttac attaagggca aagcatctct aatgacttta
    31561 gctcaacgat taataacatt ttttcattaa tttaagttgg ctaaatgcag ttaaaatttt
    31621 taacggccag ccacccaaaa tcgtcgcttc catttcccgg agtaataatg aagcatgaat
    31681 tatcaagtat gaaggcattt gtcatactgg cagagtccaa ttcatttaac aatgccgcta
    31741 aattactcaa tattacgcaa cctgcattaa cacgaagaat aaaaaagatg gaggaagatt
    31801 tacatattca actatttgag cgtacaactc gcaaggttac gttaacaaaa gcaggaaaaa
    31861 ggttgctccc agaggcgcgg gaattaataa agaaattcga tgaaacgctt tttaatattc
    31921 gtgatatgaa tgcttatcat cgtggtatgg tgacgttagc gtgtattccg accgcagtat
    31981 tttatttttt accgctggca atcggtaaat ttaatgagct gtatcccaat attaaagtgc
    32041 ggattctgga acaaggcacg aataattgca tggaatcggt gctatgtaac gaatcagact
    32101 ttggcataaa catgaacaac gtcacaaatt catccattga ttttagccca ctggtcaacg
    32161 agccgttcgt gctggcctgt cggcgtgacc atccattggc caaaaagcag cttgtagaat
    32221 ggcaggagtt agtgggttac aaaatgattg gcgtacgctc ctccagcggc aaccgactgt
    32281 tgatagaaca acagctagcc gacaagccct ggaagctgga ctggttttac gaagtgcgtc
    32341 atctttcgac gtcattagga ctggtggagg caggactggg gatttcagcg cttcctggtc
    32401 tggcaatgcc ccatgcgccc tattcttcga ttattggtat cccactggtg gaacccgtta
    32461 tacgtcggac attggggatt attcgccgca aagatgccgt gctttctccg gcagctgaac
    32521 gcttttttgc cttgctgatt aatttgtgga ctgacgataa agacaatttg tggaccaata
    32581 tcgtcgaacg ccagagacat gcgttacagg agataggtta atacaccggc gcaggagaga
    32641 ctgcgccggg taaatcagcg gttagtgtgc gttaaccacc acccacatcg gtccctgccc
    32701 gaccgcatag cggccttttt catgcagtag cccctgctca ccaacaattt cgtataccga
    32761 gatatggtga gatttttgcc cggcggcaat cagatactta ccacggtgat cgatattaaa
    32821 gccgcgcggc tgggtttcag ttggctggaa gccctcttta ctcaacacgc tgccatcttc
    32881 cgaaacgctg aagacagtaa tcaggctggc ggtacggtca caggcatata agtgacgacc
    32941 atccggggta atatgaatat cagccgccca acgggtgtcg gagaagtttt ccggcatcat
    33001 atccagcgtc tggacacatt cgatattgcc gtgcggatct ttcagttccc agacatccac
    33061 tgagctgttt aactcattga cgcaatacgc atattgttcg tttggatgga ataccatatg
    33121 acgcgggccg gccccttcaa cggtagtcac ttccgcaggg tcctgcgcca ccagatgacc
    33181 atcatcgctg accgtaaaca ggcaaatgcg atcctgcttt aatgccggaa cccacagcgt
    33241 acggttgtcc ggtgagatat tggcggaatg gcaaccgtcc agcccctcga ccacatcgac
    33301 gacgccaact ggcaggccat cttccagacg cgttacgctc acgttacccg cattgtaaga
    33361 acctacaaag acaaactgcc cctggtgatc ggtggaaata tgcgtcggac tacccggcag
    33421 cgcagactct gcggcaaagg tcagtgcgcc atcgtccggg gcgatacgat acgccaggac
    33481 gcgaaactca gggcgaacac caacatagag ataacgtttg tccgggctga ccaccatcgg
    33541 ctgcacctgc cccggcacat cgacaacctg tgtcagcgtc agtgcgcctt catgattcag
    33601 gttccagacg tgaatttgct ggctctcagg gctggcgata taaactgttt gcttcatgaa
    33661 tgctcctttg catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa
    33721 tgctgaatta aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagaa
    33781 gaaaattctt aatcttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt
    33841 agacggcacc ttattgaccc cgaaaaagac cctgctacct tcatcgatag aagccctggc
    33901 ccgcgctcgc gaagcaggtt atcaattaat catcgtcaca ggtcgccatc acgtcgctat
    33961 tcatcctttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
    34021 tttgtatgat tatcatgcaa aaactgtgct tgaagccgat ccaatgcctg ttaacaaagc
    34081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
    34141 cgcgatggtt tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
    34201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacggcgca
    34261 acaagttaac gccgtatgga agttcgccct cacgcacgat gacctgccgc aattgcagca
    34321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
    34381 ggttgatatt gcacgcggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
    34441 gcaaggctgg tcgatggaaa acgtcgtggc gttcggcgat aactttaatg atatcagtat
    34501 gctggaggcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
    34561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
    34621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
    34681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
    34741 cgacctccag ttccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
    34801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
    34861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgtc cagcttattg acccacaaat
    34921 gctgatcgcc cagcgccagc gcagtcatcg cgtaatgcgg atgatgctca agtaccgtca
    34981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactacccc
    35041 agacttcttc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
    35101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcggg atgttgattt
    35161 cccgcgtcag acgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
    35221 ccagcggttc atccagcaac agcaattctg gtgccgtcag caaagcccgc ccaatcgcta
    35281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
    35341 ctaaaagcgc caccagctta tcgaactgat cgaccatgct tttcgccatg ccgtagcgca
    35401 gattgccacg cactttgtaa tgcgggaaca gccgcgcatc ctgaaaaaca tagccaacgc
    35461 gacgcttttc cggcgtcagg cagatacctt tctcggcatc atttagtacc cgaccattga
    35521 ggacaatcct ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
    35581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
    35641 tagtcaggca atggttgccc aacgtctggg aaaaattcag ttccagcatg attagcgccc
    35701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
    35761 cgcaatagaa ataatgcaca gtctcgctgc gccactttcc ccgccggggg tctgaatcag
    35821 ggtatacatg gcagaaggaa tggttcgcgt ttcaccagga atgttcgaca caaaggtgat
    35881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataatgcc
    35941 cggtaatgtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacccagtgt
    36001 tcttgcggcc tgttccagtt tgacatcaac tccttccagc gccagacgaa ttgcccgcac
    36061 catcagcgga aacgacatga cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa
    36121 ggtaatgcca aaccagtcat acaaacgttc accgataaat ccgcgccgtc ccatcgaaac
    36181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
    36241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
    36301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
    36361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgca
    36421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
    36481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
    36541 tttacccctt tgctggcaac tgcgtcagaa ccgtaaacaa tgcccaacgg cgcttcattg
    36601 cgttcgacca acgccagcgc gccacgaaca tcttccgccg gagccagttt cggggagagc
    36661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
    36721 tccggatcgc caaccgccag gcgaccgcca ttcagcagtg aagtccagtt ggttttgctg
    36781 tcgatggtaa aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
    36841 agcagtgtct gacgcgtagc tgtatcgatc gcttttttat caaccgcata atccatccat
    36901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
    36961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
    37021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
    37081 tctgccagtg cattgccagc aacagcgaaa gagagtgccg ccccagcaaa caagttcaac
    37141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
    37201 aactcggaat tttcccagca gttattgcta acctttgtga ggtagataag aaaaaatatc
    37261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc attcagggaa
    37321 tcaatgattt tgtccaggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
    37381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
    37441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
    37501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
    37561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
    37621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
    37681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
    37741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
    37801 caggcggtaa aggtggcggc ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
    37861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg
    37921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
    37981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
    38041 atgtcttact ggctgatgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
    38101 cgcgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
    38161 gcattactca ggacgaagcg gtcgcgcaaa acgctgacaa ccaattaccg ggtattatta
    38221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccg gacgggcaaa
    38281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaagga cagaatgtca
    38341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
    38401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
    38461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
    38521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagcgggaaa
    38581 tcggccctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
    38641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
    38701 tggcagcgga ataacaccga tatgctcggc cctggcgaag atgacaccgg acgcactacg
    38761 gctgagatta ttcaggatga agtaaaggat gcaccgcgct gcatgcaact ggcacagcag
    38821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
    38881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
    38941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
    39001 cagtccggta ttactctggt actggtgctc aatcgcttcg atgagatccc ggagtttgtc
    39061 cagtttgctg gcgtgctggc ggattgcacg ttagcggaaa ctggcgctaa agaggaactg
    39121 ctccaacaag cactcgtcgc gcaactggcg catagtgaac agcttgaagg tgtgcaactg
    39181 ccggagccgg atgaaccttc agcacgtcac gccttaccca ccaacgaacc gcgcattgtg
    39241 ctgaataatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
    39301 gtgaatcctg gcgagcactg gcaaattgtc gggccaaatg gtgcgggaaa atcgacgtta
    39361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcgga
    39421 cgacgtcgtg gcagcggcga aaccatctgg gatatcaaaa agcatatcgg ttacgtcagc
    39481 agtagtttgc atctggatta ccgggtcagt actactgtgc gtaatgtgat tctttctggc
    39541 tattttgatt cgattggcat ttatcaggcc gtttcggatc gccagcaaaa actggtgcag
    39601 cagtggctgg atattctcgg cattgataaa cgcacggctg acgctccgtt ccatagtctt
    39661 tcctggggac agcagcgtct ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
    39721 cttattctcg acgaaccatt gcaggggctt gatccgctga atcgccagct tattcgccgt
    39781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccatgct
    39841 gaagatgcgc cagcttgtat tacccatcgt ctggagttcg tgccggacgg tggcctctat
    39901 cattatgcgc tgacaaaaat aaactaagtc ggtaatgctg actgtgccgg atgcggcgta
    39961 aacgctttat ccggcacacg gtcagacgca tccaggcatg aaactgcgtc tttttttcag
    40021 ataaaaagcg caatcattca caatccctct gttttataat cacttaatcg cgcataaaaa
    40081 acggctaaat tcttgtgtaa acgattccac taatttattc catgtcacac ttttcgcatc
    40141 tttgttatgc tatggttatt tcataccata agcctaatgg agcgaattat gagagttctg
    40201 gtcaccggtg gtagcggtta cattggaagt catacctgtg tgcaattact gcaaaacggt
    40261 catgatgtca tcattcttga taacctctgt aacagtaagc gcagcgtact gcctgttatc
    40321 gagcgtttag gcggcaaaca tccgatgttt gttgaaggcg atatccgtaa cgaagcgttg
    40381 atgaccgaga tcctgcacga tcacgctatc gacaccgtga tccacttcgc cgggctgaaa
    40441 gccgttggcg aatcggtaca aaaaccgctg gaatattacg acaacaatgt caacggtact
    40501 ctgcgcttga ttagcgccat gtgcgccgct aacgtcaaaa actttatttt tagctcctcc
    40561 gccaccgttt atggcgatca gcccaaaatt ccatacgttg aaagcttccc gaccggcaca
    40621 ccgcaaagcc cttacggcaa aagcaagctg atggtggaac agatcctcac cgatctgcaa
    40681 aaagcccagc cggactggag cattgccctg ctgcgctact tcaacccggt tggcgcgcat
    40741 ccgtcgggcg atatgggcga agatccgcaa ggcattccga ataacctgat gccatacatc
    40801 gcccaggttg ctgtaggccg tcgcgactcg ctggcgattt ttggtaacga ttatccgacc
    40861 gaagacggta ctggcgtacg cgattacatc cacgtaatgg atctggcgga cggtcacgtc
    40921 gtggcgatgg aaaaactggc gaacaagcca ggcgtacaca tctacaacct cggtgctggc
    40981 gtaggcagca gcgtgctgga cgtggttaat gccttcagca aagcctgcgg caaaccggtt
    41041 aactatcatt ttgcaccgcg tcgcgagggc gaccttccgg cctactgggc ggacgccagc
    41101 aaagccgacc gtgaactgaa ctggcgcgta acgcgcacac tcgatgaaat ggcgcaggac
    41161 acctggcact ggcagtcacg ccatccacag ggatatcccg attaaggaac gaccatgacg
    41221 caatttaatc ccgttgatca tccacatcgc cgctacaacc cgctcaccgg gcaatggatt
    41281 ctggtttcac cgcaccgcgc taagcgcccc tggcaggggg cgcaggaaac gccagccaaa
    41341 caggtgttac ctgcgcacga tccagattgc ttcctctgcg caggtaatgt gcgggtgaca
    41401 ggcgataaaa accccgatta caccgggact tacgttttca ctaatgactt tgcggccttg
    41461 atgtctgaca cgccagatgc gccagaaagc aacgatcctt tgatgcgttg ccagagcgcg
    41521 cgcggtacca gccgggtgat ctgcttttca ccggatcaca gtaaaacgct gccagagctc
    41581 agtgttgcgg cattgacgga aatcgtcaaa acctggcagg agcaaaccgc agaactgggg
    41641 aaaacgtacc catgggtgca ggtctttgaa aataaaggcg cggcgatggg ctgctctaac
    41701 ccgcatccgc acggacaggt ttgggcaaat agcttcctgc ctaacgaagc tgagcgcgaa
    41761 gaccgcctgc aaaaagaata ttttgccgag cagaaatcac caatgctggt ggattatgtt
    41821 cagcgcgagc tggcagacgg tagccgtacc gttgttgaaa ccgaacactg gttagccgta
    41881 gtgccttact gggctgcctg gccgttcgaa acgctactgc tgcccaaagc ccacgtttta
    41941 cggatcaccg atttgaccga cgcccagcgc agcgatctgg cgctggcgtt gaaaaagctg
    42001 accagtcgtt atgacaacct cttccagtgc tccttcccct actctatggg ctggcacggc
    42061 gcgccattta atggcgaaga gaatcaacac tggcagctgc acgcgcactt ttatccgcct
    42121 ctgttgcgct ccgccaccgt acgtaaattt atggttggtt atgaaatgct ggcagagacc
    42181 cagcgagacc tgaccgcaga acaggcagca gagcgtttgc gcgcagtcag cgatatccat
    42241 tttcgcgaat ccggagtgta agaaatgagt ctgaaagaaa aaacgcaatc tctgtttgcc
    42301 aacgcatttg gctaccctgc cactcacacc attcaggcgc ctggccgcgt gaatttgatt
    42361 ggtgaacaca ccgactacaa cgacggtttc gttctgccct gcgcgattga ttatcaaacc
    42421 gtgatcagtt gtgcgccacg cgatgaccgt aaagttcgcg taatggcagc cgattatgaa
    42481 aatcagctcg acgagttttc cctcgatgcg cccattgtcg cgcatgaaaa ctatcaatgg
    42541 gcgaactacg ttcgtggcgt ggtgaaacat ctgcaactgc gtaacaacag cttcggcggc
    42601 gtggacatgg tgatcagcgg caatgtgccg cagggtgccg ggttaagttc ttccgcttca
    42661 ctggaagtcg cggtcggaac cgtattgcag cagctttatc atctgccgct ggacggcgca
    42721 caaatcgcgc ttaacggtca ggaagcagaa aaccagtttg ttggctgtaa ctgcgggatc
    42781 atggatcagc taatttccgc actcggcaag aaagatcatg ccttgctgat tgactgtcgc
    42841 tcactgggga ccaaagcagt ttccatgccg aaaggtgtgg ctgtcgtcat catcaacagt
    42901 aacttcaaac gtaccctggt tggcagcgaa tacaacaccc gtcgtgaaca gtgcgaaacc
    42961 ggtgcgcgtt tcttccagca gccagctctg cgcgatgtca ccattgaaga gttcaacgct
    43021 gttgcacatg agctggaccc aatcgtggcg aaacgcgtgc gtcatatcct gactgaaaac
    43081 gcccgcaccg ttgaagctgc cagcgcgctg gagcaaggcg acctgaaacg tatgggcgag
    43141 ttgatggcgg agtctcatgc ctctatgcgc gatgatttcg aaatcaccgt gccgcaaatt
    43201 gacactctgg tagaaatcgt caaagctgtg attggcgaca aaggtggcgt acgcatgacc
    43261 ggcggcggat ttggcggctg tatcgtcgcg ctgatcccgg aagagctggt gcctgccgta
    43321 cagcaagctg tcgctgaaca atatgaagca aaaacaggta ttaaagagac tttttacgtt
    43381 tgtaaaccat cacaaggagc aggacagtgc tgaacgaaac tcccgcactg gcacccgatg
    43441 gtcagccgta ccgactgtta actttgcgta acaacgcagg gatggtagtc acgctgatgg
    43501 actggggtgc gactttactt tcagcccgta ttccgctttc cgatggcagc gtccgcgagg
    43561 cgctgctcgg ctgtgccagc ccggaatgct atcaggatca ggccgcgttt ctgggggcct
    43621 ctattggtcg ttatgccaac cgcatcgcca atagccgtta tacctttgac ggtgaaaccg
    43681 tgacgctttc gccaagtcag ggcgttaacc agctgcacgg cggcccggaa gggttcgaca
    43741 aacgtcgctg gcagattgtg aaccagaacg atcgtcaggt gctgtttgcc ctgacttcag
    43801 atgatggcga tcagggcttc ccgggtaatc tcggcgcgac ggtgcaatat cgtctgaccg
    43861 acgataaccg tatctccatt acttatcgcg ccacagttga taaaccttgc ccggtgaata
    43921 tgactaatca cgtctatttc aatcttgacg gcgagcagtc tgacgtgcgc aatcacaagt
    43981 tgcagattct ggcggacgaa tatctgccgg ttgatgaagg cggcattccg cacgacggcc
    44041 tgaaatctgt cgccggaacg tcttttgatt tccgcagcgc caaaatcatc gccagtgagt
    44101 ttcttgccga cgacgatcag cgcaaagtaa aaggttacga tcacgcgttc ttgttacagg
    44161 ccaaaggcga tggcaagaaa gtggcggcgc atctctggtc agcagatgaa aaattgcagc
    44221 tgaaggtcta caccactgct ccggctctgc aattttactc cggcaatttc ctcggcggca
    44281 caccgtcgcg gggaaccgaa ccttacgccg actggcaagg gctggctctg gaaagcgagt
    44341 ttctgccgga cagcccgaac caccctgaat ggccgcaacc ggactgcttc ctgcatcctg
    44401 gtgaagagta ttccagcctg acggaatatc agtttattgc tgagtaagtt taagccctcg
    44461 tcatgagggc tttatctcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    44521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    44581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    44641 gtaagtatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    44701 aagaaaaccg tttcaccggt tggtacgacg tggatctttc tgagaaaggc gtaagcgaag
    44761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    44821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    44881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    44941 tgaacaaagc ggaaaccgcc gaaaagtatg gcgacgagca ggtgaaacaa tggcgtcgtg
    45001 gttttgccgt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    45061 cgcgttacgc gaaactgagc gagaaagagc tgccgctgac tgaaagcctg gcgctgacca
    45121 ttgaccgcgt gatcccttac tggaatgaaa ctattctacc gcgtatgaag agcggtgagc
    45181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    45241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    45301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcag
    45361 cgaaagcagc ggcggttgct aaccagggta aagcgaagta aacgtcattc gtttaaaatg
    45421 agaaagccga ctgcaagtga gtcggctttt ttgttgctaa caatggagca cattgcctga
    45481 tgcggcgctg cgcgtcttat caggcctacg gtgattcatc ggatacgcca ctctggcggt
    45541 gcatccgaca attaaacctt acccgcgacg cgcttttact gcattcgcca gttgacgtaa
    45601 cagagcatcg gtatcttccc agccaatgca ggcatcagtg atgctcttac cgtaggccag
    45661 cggctccccg ctctccaggc tctgattgcc ttcaaccaga tgactttcca ccatcacgcc
    45721 aataatggcc ttttcgccac cggcaatctg ctggcaaacg tcagcacaaa catccatctg
    45781 ctttttgaat tgttttgacg agttagcatg gctgaaatcg atcatcacct gtgctggcag
    45841 gcctgctttg ttcagccctt ctttcacttc agcaacgtgc ttcgcgctgt agttaggctc
    45901 tttaccgccg cgcagaatga tatggcaatc gccgttaccg ctggtattca caatcgccga
    45961 atgcccccat ttcgttacgg acaggaagca gtgcggcgca ccggcggcat taatggcatc
    46021 gatagccact ttaatcgtac cgtcggtgcc atttttgaag ccgaccggac aagaaagccc
    46081 tgatgccagt tcgcggtgca cctgcgattc ggtggtacgt gcgccaattg cgccccagct
    46141 catcaggtca gcgagatatt gtggggtgat catatcgaga aactcacccg ccgctggcag
    46201 accgctgtcg ttaatatcaa gcagcaattt acgggcaata cgcaaaccgt cgttgatctg
    46261 gaagctattg tccatatgcg gatcgttaat cagccctttc cagcccaccg tggtgcgcgg
    46321 cttttcaaaa tagacgcgca ttacgatttc cagctcatct ttcagctctt cacgcagcgc
    46381 cagcaagcga gtggcgtact ctttagccgc tacaggatca tgaattgagc acggaccaat
    46441 caccactaac aggcgatcat cattcccttt caggatttta tggatcgctt ttcgggcatg
    46501 ggcaaccgta ttagcggcat tttcagtagc ggggaatttt tccagcaatg cgacaggagg
    46561 aagtaactct ttgatttctt tgatgcgtaa atcgtcgttc tgataattca tgtctgttcc
    46621 agtgttgcca tacttatctt agtgaatgca atacctccaa tctatatctt ccgtcagaat
    46681 gtgtaaacgg ggttttacac tatgaacgga ttaatcctgg aattagcaag aaaaacgcca
    46741 gattgtcgcg aaaaacgaga tctctcctac aatttctaac tgtaactcct ttgtttgtta
    46801 attatttcaa gattctctgc tggatttctt tgcctggctg aaatcttaaa ccaatgcctt
    46861 atattcacct gcaaatgcac tgttggaaga ggttatccga cataacgacc ataacaggag
    46921 catcctatga aaatgacaaa actggccaca ttatttctga ctgccactct aagccttgcc
    46981 agcggtgccg cactggctgc cgatagcgga gcgcaaacta ataacggcca ggcaaacgcc
    47041 gccgctgatg cgggccaggt agcccctgac gcccgtgaaa atgtcgcgcc aaataacgtc
    47101 gacaataacg gggtaaatac gggttctggc ggcacaatgc tgcatccgga tggttcttca
    47161 atgaacaatg acggcatgac caaagatgaa gagcacaaga acaccatgtg caaagatggt
    47221 cgctgtccgg atatcaataa aaaggtacaa accggtgatg gcatcaataa tgatgtcgat
    47281 accaaaaccg acggtaccac gcagtaaccc tgcaaaatag ccaataatgg gagagcggag
    47341 tctctccctt ttaatttttc ccttctaaaa gcagtatgat gtcataaagt taagtgagaa
    47401 taattaaggg atgactcgtc atggcgcact cacactcaca cacgtcctca cacctgccag
    47461 aagataataa tgctcgtcgc ttgttgtatg ctttcggcgt gactgccggg tttatgctgg
    47521 tagaagtcat tggtggtttt ctttctggtt ctctggcttt gctggccgat gcgggtcata
    47581 tgttgaccga tactgccgcc ctgcttttcg ccctactcgc cgtgcaattt tcacgtcgtc
    47641 ctcccaccat tcgccacact ttcggttggc taagactgac cacgctcgcg gcgtttgtga
    47701 atgccatcgc cctcgtggta attaccattt tgattgtctg ggaggcgata gaacgcttcc
    47761 gcacgccgcg tccggtcgag ggcggcatga tgatggcaat tgccgtggct gggttgctgg
    47821 caaatatact ttctttctgg ttacttcatc acggcagcga agagaaaaac ctcaacgtac
    47881 gagcagcggc actgcatgtg ctcggggacc tgctgggttc ggttggggct attattgccg
    47941 cgttgattat tatctggacc ggctggacac ctgctgaccc cattctctcg atactggtgt
    48001 cgcttctggt tctgcgtagc gcgtggcgac tgttgaaaga tagcgtgaat gaattacttg
    48061 agggtgcacc ggtatcgctg gatatcgctg aactgaagcg tcgtatgtgc cgggaaatcc
    48121 cggaagtccg caatgtgcac catgtgcatg tatggatggt aggcgagaag ccggtgatga
    48181 cgttgcatgt gcaggtgatc ccgccacacg atcacgatgc cttgttggat cagatccaac
    48241 actacctgat ggatcactat cagattgagc acgccaccat tcagatggaa tatcaacctt
    48301 gtcatgggcc agactgccac ctgaatgaag gggtgtctgg ccattcacat catcaccatt
    48361 aatgggacag cgcgcgtgag cctctttcac gtgcgctgtt gatccacatc cggctgccgt
    48421 tgagcgcaat aaaggtcagg atgatgtact ccagagacat ggcgtaaacg ccctgaagtg
    48481 caaaaataac gacgctaatc acgttaataa tcacccacaa cagccagttt tccacatact
    48541 tacgcgtcat cagaatcatt gccacgatag ataacaccat catgcatgaa tcccagaacg
    48601 ggaaagcgtc cggttgcagt tcaggcatca caacctgtaa tcctaatgct tgcatgatga
    48661 tgactgccac gcgggtcaaa aatgcaaaca ccggattgat aaataccgtc atcagaccaa
    48721 tagcaatgac acaaaccgcc agccaggtta atgccttcgg caatggtaac cagcgaattt
    48781 tcaactccgc ctcgttctga ctggtttgtc gcgaccacgc ataccagccg taaatattcg
    48841 cggcaaagaa aaacacctgt aatagcaggc tggcatacag ctgaatctga aagaaaataa
    48901 tgccaaacaa ggtgacgttg atcaggccaa agaagtagtt gctgatcttc tccagactgg
    48961 caaggccaat acacagcaac ccggcgatcg tgcctaccgc ttcgatccat gagagatcat
    49021 aaccgcctgc ccctattggt atatgtacca ggatattctg cacactaaaa aaatccatct
    49081 ttcccccaaa gcgtaacgtt acgccttatt attcgttatc cacgtagtgt agccgcaaaa
    49141 tccagcatac gattgagcgg caccagcgcc ctctctcgca gcctctcatc aacatgaacc
    49201 tcgtgattac ttccttcctg ttctaatgcc tcagcgatag cctgaaggcc attcatggcc
    49261 atccacggac aatgcgcgca gctgcggcag gttgcgccct cacccgcggt tggtgcttcc
    49321 agtaactctt tatctggcac cgcctgctgc attttgtaga aaatgccccg atcggttgcc
    49381 acgataagcc gctgatgtgg caatgttttt gcagcagcga tcagttgact ggtggaaccg
    49441 accgcatccg ccatatcgac aatagcttgt ggtgattctg gatgcaccag tatggcagca
    49501 tccgggtatt cttcttgcaa gcgggttaac gcctgagtct taaattcatc atgcacaatg
    49561 caagcaccct gccagcataa aatgtctgcg cccgtctgtt tttgcacgta acgccccaga
    49621 tgtttgtcgg gtgcccagat gattttttca cccaaactat caagatgatc aataagttcg
    49681 acggcaatgc ttgaagttac cacccaatct gcgcgcgctt ttaccgcagc agaagtgttg
    49741 gcgtagacga cgacagtacg atcgggatgg gcatcgcaaa atgcgttaaa ttcttcaaca
    49801 gggcagccga gatccagtga acactcagcc tgaagtgtcg gcatcagaat tgttttttcc
    49861 ggactgagaa ttttggcggt ttctcccata aatctcaccc cagcgactaa caacgtagaa
    49921 gcgggatgct ttgcaccgaa gcgcgccatt tccaaagaat cagaaataca gccaccggtt
    49981 tcttctgcca gttgttgaat ttcgggatcg gtatagtagt gggcaaccat caccgcatta
    50041 cgttctttta gcagacgttt tatcttctcg cggtaatacg ctttttcatc aatgcttaac
    50101 ggcgtcggct tcggggggaa aggataaatc gccgtgtctg gatcaaacat cacgctcatc
    50161 ttaccatctc gttttacagg cttaacgtta aaaccgacat tagcgtgata ttcgctgtta
    50221 tcggtgtaat ttgttttata tgctaaacaa gatagtcgaa tggtggggaa aagtcactcg
    50281 aattacgtat gagatgccga atgccacact ttgcgtctta ttcctccgac gtaaagttgt
    50341 ttacgcagca agcgcggagt atgtcatcag ggtcaagaat tgaaattgag agatgcctga
    50401 atagcaaaaa agcgccttta gggcgctttt ttacattggt gggtcgtgca ggatgactcg
    50461 cttcgctcgc ccttcgggcc gtcgccgcga gcggctccgt tgtttcgcta cgctcaactc
//
LOCUS       DAZFKF010000026        50461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFKF010000026 DAZFKF010000000
VERSION     DAZFKF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.026"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            65..766
                     /gene="elfD"
                     /locus_tag="ACHKA1_001876"
     CDS             65..766
                     /gene="elfD"
                     /locus_tag="ACHKA1_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5679533.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            791..3391
                     /gene="elfC"
                     /locus_tag="ACHKA1_001877"
                     /pseudo
     CDS             791..3391
                     /gene="elfC"
                     /locus_tag="ACHKA1_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3382..4452
                     /gene="elfG"
                     /locus_tag="ACHKA1_001878"
     CDS             3382..4452
                     /gene="elfG"
                     /locus_tag="ACHKA1_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5679534.1"
                     /translation="MQIIFGEKCVSLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRNGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGGDTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARINVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4464..5006
                     /gene="ycbU"
                     /locus_tag="ACHKA1_001879"
     CDS             4464..5006
                     /gene="ycbU"
                     /locus_tag="ACHKA1_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5679535.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANIVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5014..5529
                     /gene="ycbV"
                     /locus_tag="ACHKA1_001880"
     CDS             5014..5529
                     /gene="ycbV"
                     /locus_tag="ACHKA1_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5679536.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5495..6232
                     /gene="ycbF"
                     /locus_tag="ACHKA1_001881"
     CDS             5495..6232
                     /gene="ycbF"
                     /locus_tag="ACHKA1_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO5679537.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNVGVVPPKSLRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6343..7353
                     /gene="pyrD"
                     /locus_tag="ACHKA1_001882"
     CDS             6343..7353
                     /gene="pyrD"
                     /locus_tag="ACHKA1_001882"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO5679538.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7527..8069
                     /gene="zapC"
                     /locus_tag="ACHKA1_001883"
     CDS             7527..8069
                     /gene="zapC"
                     /locus_tag="ACHKA1_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO5679539.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8066..9175)
                     /gene="ycbX"
                     /locus_tag="ACHKA1_001884"
     CDS             complement(8066..9175)
                     /gene="ycbX"
                     /locus_tag="ACHKA1_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO5679540.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PEAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILTTAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9419..11527
                     /gene="rlmKL"
                     /locus_tag="ACHKA1_001885"
     CDS             9419..11527
                     /gene="rlmKL"
                     /locus_tag="ACHKA1_001885"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO5679541.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11539..13446
                     /gene="uup"
                     /locus_tag="ACHKA1_001886"
     CDS             11539..13446
                     /gene="uup"
                     /locus_tag="ACHKA1_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5679542.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13576..14829
                     /gene="pqiA"
                     /locus_tag="ACHKA1_001887"
     CDS             13576..14829
                     /gene="pqiA"
                     /locus_tag="ACHKA1_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO5679543.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14834..16474
                     /gene="pqiB"
                     /locus_tag="ACHKA1_001888"
     CDS             14834..16474
                     /gene="pqiB"
                     /locus_tag="ACHKA1_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO5679544.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16471..17034
                     /gene="pqiC"
                     /locus_tag="ACHKA1_001889"
     CDS             16471..17034
                     /gene="pqiC"
                     /locus_tag="ACHKA1_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO5679545.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17290..17457
                     /gene="rmf"
                     /locus_tag="ACHKA1_001890"
     CDS             17290..17457
                     /gene="rmf"
                     /locus_tag="ACHKA1_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO5679546.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17527..18045)
                     /gene="fabA"
                     /locus_tag="ACHKA1_001891"
     CDS             complement(17527..18045)
                     /gene="fabA"
                     /locus_tag="ACHKA1_001891"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO5679547.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFNKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18114..19874)
                     /gene="ycbZ"
                     /locus_tag="ACHKA1_001892"
     CDS             complement(18114..19874)
                     /gene="ycbZ"
                     /locus_tag="ACHKA1_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5679548.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARALQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPILIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20060..20512
                     /gene="matP"
                     /locus_tag="ACHKA1_001893"
     CDS             20060..20512
                     /gene="matP"
                     /locus_tag="ACHKA1_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO5679549.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20588..21643)
                     /gene="ompA"
                     /locus_tag="ACHKA1_001894"
     CDS             complement(20588..21643)
                     /gene="ompA"
                     /locus_tag="ACHKA1_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO5679550.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IDNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKAHNNVTGESEKNHDTGVSPVFAGGVEWAITPE
                     IATRLEYQWTNNIGDAHTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTK
                     HFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQ
                     GLSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRR
                     VEIEVKGIKDVVTQPQA"
     gene            complement(22000..22509)
                     /gene="sulA"
                     /locus_tag="ACHKA1_001895"
     CDS             complement(22000..22509)
                     /gene="sulA"
                     /locus_tag="ACHKA1_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO5679551.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLPPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            22728..23357
                     /gene="sxy"
                     /locus_tag="ACHKA1_001896"
     CDS             22728..23357
                     /gene="sxy"
                     /locus_tag="ACHKA1_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO5679552.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23320..25482)
                     /gene="yccS"
                     /locus_tag="ACHKA1_001897"
     CDS             complement(23320..25482)
                     /gene="yccS"
                     /locus_tag="ACHKA1_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO5679553.1"
                     /translation="MAYMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(25492..25938)
                     /gene="yccF"
                     /locus_tag="ACHKA1_001898"
     CDS             complement(25492..25938)
                     /gene="yccF"
                     /locus_tag="ACHKA1_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO5679554.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26061..28115
                     /gene="helD"
                     /locus_tag="ACHKA1_001899"
     CDS             26061..28115
                     /gene="helD"
                     /locus_tag="ACHKA1_001899"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO5679555.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28147..28605)
                     /gene="mgsA"
                     /locus_tag="ACHKA1_001900"
     CDS             complement(28147..28605)
                     /gene="mgsA"
                     /locus_tag="ACHKA1_001900"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO5679556.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28701..29363)
                     /gene="yccT"
                     /locus_tag="ACHKA1_001901"
     CDS             complement(28701..29363)
                     /gene="yccT"
                     /locus_tag="ACHKA1_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO5679557.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29536..29949
                     /gene="yccU"
                     /locus_tag="ACHKA1_001902"
     CDS             29536..29949
                     /gene="yccU"
                     /locus_tag="ACHKA1_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO5679558.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29994..30311)
                     /gene="hspQ"
                     /locus_tag="ACHKA1_001903"
     CDS             complement(29994..30311)
                     /gene="hspQ"
                     /locus_tag="ACHKA1_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO5679559.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30369..31559)
                     /gene="rlmI"
                     /locus_tag="ACHKA1_001904"
     CDS             complement(30369..31559)
                     /gene="rlmI"
                     /locus_tag="ACHKA1_001904"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO5679560.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCNQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31654..31932
                     /gene="yccX"
                     /locus_tag="ACHKA1_001905"
     CDS             31654..31932
                     /gene="yccX"
                     /locus_tag="ACHKA1_001905"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO5679561.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31929..32258)
                     /gene="tusE"
                     /locus_tag="ACHKA1_001906"
     CDS             complement(31929..32258)
                     /gene="tusE"
                     /locus_tag="ACHKA1_001906"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO5679562.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32349..33008)
                     /gene="yccA"
                     /locus_tag="ACHKA1_001907"
     CDS             complement(32349..33008)
                     /gene="yccA"
                     /locus_tag="ACHKA1_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO5679563.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33416..34435)
                     /locus_tag="ACHKA1_001908"
     CDS             complement(33416..34435)
                     /locus_tag="ACHKA1_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5679564.1"
                     /translation="MGRPRKNKKDNALPPRVRSNGYSYVWKPEGSTKTIGLGRVRETS
                     IAKVWQNYELEKAKRHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQRALLAVFGK
                     VLADNVKIEQVRIFMDKRGIESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFT
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSGTCEYVINTTKGGKVIAKTLNNWWNQAKHAA
                     EKKSGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(34404..34655)
                     /locus_tag="ACHKA1_001909"
     CDS             complement(34404..34655)
                     /locus_tag="ACHKA1_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO5679565.1"
                     /translation="MYELTLSPAEIQEITRYERYTKQQQQLRLHGIPFVPGPKNEPIV
                     LRSDVPRGLTAIPKVSELVSADPDFEALNNGKTKKKQKR"
     gene            complement(34722..37160)
                     /locus_tag="ACHKA1_001910"
     CDS             complement(34722..37160)
                     /locus_tag="ACHKA1_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO5679566.1"
                     /translation="MSTDKEEFSLYCEAKNDKVRKRLGIKGGFYWTTAKKLSVAISRC
                     VVAMDEAGYDADDFKKPVRVHFPVVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQDKPASTDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPDLVHTLDTLD
                     VEAAAATLPMDFNIYEIPVSVYRRAKEIVLKKESPFREWSAALRATPGILDYSRAAIF
                     ALIRSAHPEFYHYPGRLQGYINANLTETDHENPTAETLMAARHTPEKDILEEINGGLA
                     AKCKTEEEKNDEENPQPSGAMADEQATAETMAADTVEHHQDPQPLDDKSQVKVTAEEV
                     NKIMEAADISRPDADKLLAASRGEFVEGISDPNDSKWVKGIETCDSVNQNQQETEQNG
                     QKAEQNSPNALQNEPETKQPEPVVQQEEEKVCTACNQTGGGNCPDCGAVMGDATYQET
                     FNEESQDEAREKDPEEMESAGLPNKECTEGDQHANGNNETGETANPLIKVNGHREITS
                     TSRLWHHLMIDLETMGKNPDAPIASIGAVFFDPQTGEQGPEFSKIIDMGTCGGTVDIS
                     TIEWWLQRSGEARAAILADRIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNVILR
                     RSYERQEIPCPWRYTNDRDVRTIVALGLVMDFDARSVITFEGERHNALHDARYQAKYV
                     SAIWQKLFPNQADF"
     gene            complement(37253..37441)
                     /locus_tag="ACHKA1_001911"
     CDS             complement(37253..37441)
                     /locus_tag="ACHKA1_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO5679567.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYASKESCLRARDEQKISGE
                     CLPLKKVSLYLNNEIPAG"
     gene            complement(37438..37626)
                     /gene="dicB"
                     /locus_tag="ACHKA1_001912"
     CDS             complement(37438..37626)
                     /gene="dicB"
                     /locus_tag="ACHKA1_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO5679568.1"
                     /translation="METLLPNVNTSEGCFDIGILLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLKLGIFGGQQ"
     gene            complement(37637..38491)
                     /locus_tag="ACHKA1_001913"
     CDS             complement(37637..38491)
                     /locus_tag="ACHKA1_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5679569.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKAGAGRGNPMMTTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQPKGWPVSVVTGISTPVRLTTLMVVESLGGELFKLTIEAAIMAT
                     IPALSHPDVTIENGRAVTTSIAIAEFFGKRHERVLDKIRNLDCSAKFTEHNFVSSEYT
                     DSTGRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKELRQNNAPSPDKM
                     IHGDGRTLVIRLDEHGNIKFTETVPDGAMVCTLDTFQFYLEKQGWTLVNRSAIKNMTV
                     EQLLKIHC"
     gene            complement(39010..39162)
                     /locus_tag="ACHKA1_001914"
     CDS             complement(39010..39162)
                     /locus_tag="ACHKA1_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO5679570.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRW
                     LARNTN"
     gene            39437..39739
                     /locus_tag="ACHKA1_001915"
     CDS             39437..39739
                     /locus_tag="ACHKA1_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO5679571.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQ
                     YRTASILKNRRVVFNIKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            39742..40101
                     /locus_tag="ACHKA1_001916"
     CDS             39742..40101
                     /locus_tag="ACHKA1_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5679572.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(40148..40540)
                     /locus_tag="ACHKA1_001917"
     CDS             complement(40148..40540)
                     /locus_tag="ACHKA1_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5679573.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            40667..40927
                     /locus_tag="ACHKA1_001918"
     CDS             40667..40927
                     /locus_tag="ACHKA1_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5679574.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANEQAAAGVDS"
     gene            40924..41361
                     /locus_tag="ACHKA1_001919"
     CDS             40924..41361
                     /locus_tag="ACHKA1_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5679575.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            41448..42458
                     /locus_tag="ACHKA1_001920"
     CDS             41448..42458
                     /locus_tag="ACHKA1_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO5679576.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNGDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAERKVFTQVQWEQKFARHVQHVRAQVKPVSKGGSHAASGGTAS
                     RAVQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWC
                     DD"
     gene            42451..42912
                     /locus_tag="ACHKA1_001921"
     CDS             42451..42912
                     /locus_tag="ACHKA1_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5679577.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKYPKGPTPIEWLKQEYERRKAAGFI"
     gene            42946..43707
                     /locus_tag="ACHKA1_001922"
     CDS             42946..43707
                     /locus_tag="ACHKA1_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5679578.1"
                     /translation="METVFDALKAMGKASSVGLAERLDISREEVLNELWELKRNGVVD
                     KAGHIWFLAGEGESGVTEEQPEQSEVPDVLTGEVEQKVTADMMIEFIGQEGAKTCEEL
                     ADKFGVSIRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAVPVTENDGKA
                     FPQPAGVALPVQESATQEDIKTETVADIVQSLPSFTETQADELIFLSLCRANLALRRA
                     KSDVQKWERVCAALRELNKHRDILRDITATRELLR"
     gene            43740..44021
                     /locus_tag="ACHKA1_001923"
     CDS             43740..44021
                     /locus_tag="ACHKA1_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5679579.1"
                     /translation="MILRQCAGTMTVESIGSLIGRSGAAVRTKARELGICMMLRGDYH
                     QSAKCSQRDIELARQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRRVQA"
     gene            44018..44323
                     /locus_tag="ACHKA1_001924"
     CDS             44018..44323
                     /locus_tag="ACHKA1_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO5679580.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHRAEEALKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEKN"
     gene            44310..44960
                     /locus_tag="ACHKA1_001925"
     CDS             44310..44960
                     /locus_tag="ACHKA1_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5679581.1"
                     /translation="MRKINYQTLREIAKLATQGEWVAFISPGTDTYAVHTPGDERCGD
                     IIKWPGFDDQKNAENNAEFIAAFNPEVVQALLDEREAQSKRIAELEANLVALAAENAL
                     ARKAVQAFCDVVGDNTEVICEEVGRDGVLVILEAMKATGNMPATDAFLSEVRALGVDA
                     AIEAAKNLVAQEYEYKDFKAAQSDCCMHPGSDLIGKVEMTEWLVDFAAQLRKGVQS"
     gene            44957..45286
                     /locus_tag="ACHKA1_001926"
     CDS             44957..45286
                     /locus_tag="ACHKA1_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679582.1"
                     /translation="MRASISYVDDCHLSVRVDEIVSSVPTFPTKNAAVNAGAPFGWRT
                     AVRIERRFENVWVVGKKCFQSDRSAGLNFEAYRFPFLRWEKEGGITKCPILSVRRFKQ
                     EATSEQD"
     gene            45273..45617
                     /locus_tag="ACHKA1_001927"
     CDS             45273..45617
                     /locus_tag="ACHKA1_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5679583.1"
                     /translation="MSKIDYQALREAAQNYRSMLAWYQEKPDSPNAEQDCDAALAAFK
                     REIRHREVGIIADLLDELEEAKQRIDEQESRIVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTAAGIRIEGE"
     gene            45619..45972
                     /locus_tag="ACHKA1_001928"
     CDS             45619..45972
                     /locus_tag="ACHKA1_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679584.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAKP
                     VASCIIEDGGMCVDGFGEYVGHSLPDGTHQLYAAPPVPSKLPREYRNGWPLAYSDYAE
                     GWNDCREAMLQGDKS"
     gene            45969..46322
                     /locus_tag="ACHKA1_001929"
     CDS             45969..46322
                     /locus_tag="ACHKA1_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5679585.1"
                     /translation="MINRIKLEHILEYARQQKHTGQHCKIPPGDMVEIMEIAMRKAGN
                     SPVTPDGWISCSERMPPVGVDVLLFVSSTGEQFTGFNLDNTGDFQYAQCFGAPIVCEA
                     THWMPLPEPPKEVRQ"
     gene            complement(46525..46824)
                     /locus_tag="ACHKA1_001930"
     CDS             complement(46525..46824)
                     /locus_tag="ACHKA1_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5679586.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNIIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGRKVFIVGIYHGGQSYETDFL"
     gene            complement(46830..47087)
                     /locus_tag="ACHKA1_001931"
     CDS             complement(46830..47087)
                     /locus_tag="ACHKA1_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO5679587.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRVAIRSGK"
     gene            47223..47501
                     /locus_tag="ACHKA1_001932"
     CDS             47223..47501
                     /locus_tag="ACHKA1_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679588.1"
                     /translation="MAQDTKLHNSDNSTAPASRHGRRSHAFKSDWFQHDPCTEEQAEW
                     LIQCYRRRGYEFQKDLSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            47503..48561
                     /locus_tag="ACHKA1_001933"
     CDS             47503..48561
                     /locus_tag="ACHKA1_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5679589.1"
                     /translation="MRVLLRPVLVPELGMVLLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISSTQERE
                     LSLAELSWWAVCNQVVDALPEAVVRRSLGLPAEKIRSVYRESDIIPGEQAATSILKQR
                     TKNIALPLHVHQQQNPPQEKTLVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            48562..48942
                     /locus_tag="ACHKA1_001934"
     CDS             48562..48942
                     /locus_tag="ACHKA1_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5679590.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLIDDEQFDEINIVR
                     GKPVSGGRLGVKIYPIMLEGQVKK"
     gene            48939..49760
                     /locus_tag="ACHKA1_001935"
     CDS             48939..49760
                     /locus_tag="ACHKA1_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5679591.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDCVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCSCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVSKFDIEEAWAERQLKKVTR"
     gene            49987..50184
                     /locus_tag="ACHKA1_001936"
     CDS             49987..50184
                     /locus_tag="ACHKA1_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO5679592.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            50335..>50461
                     /locus_tag="ACHKA1_001937"
     CDS             50335..>50461
                     /locus_tag="ACHKA1_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO5679593.1"
                     /translation="MANTVKISSCELINADCLEFIQTLPENSVDLIVTDPPYFKVK"
BASE COUNT        12999 a        11850 c        13170 g        12442 t
ORIGIN      
        1 ttattaatta gtacccctcc agtgttctgg aggggatatt catatttttt aagagtgact
       61 atttatgaaa acttgcataa caaagggaat tgtgaccgta agtttaacgg caatattact
      121 cagctgctca tccgcatggg cggcaggtaa aggcgggatt ggacttgcag caacacgtct
      181 tgtttattca gaaggtgaag agcaaatttc actgggtgta cgtaacacca gtccggatgt
      241 tccttatctt attcagtcat gggtgatgac cccagataat aaaaaatcag cagactttat
      301 tattacacca ccgctatttg tgctgaatcc ggctaatgag aatctgttac gcattatgta
      361 cattggtgcg ccgttggcga aagacagaga aactcttttc ttcactagcg tacgggcagt
      421 cccttcaaca acgaagcggg aagaaggcaa taccctgaag attgccacac aaagcgtcat
      481 caaacttttc tggcgaccaa aaggtttagc gtatccctta ggcgaggctc cggcgaaact
      541 gcgttgcact tcgtcagctg acatggttac ggtcagtaac ccaacacctt atttcattac
      601 cctgacagac ctgaaaatag gtggaaaggt agttaaaaat caaatgattt ccccctttga
      661 taaataccaa ttctctctgc caaagggtgc caaaaatagc agcgtaacgt atcgaaccat
      721 caatgactac ggggcggaaa cgccgcaact caactgtaaa tcgtaattcg tcttcagtta
      781 agagagcgag atgtatagaa ctcaccgaca acacagcctg ttaagctctg gtggcgtgcc
      841 atcgtttatt ggtgggctgg tggtgtttgt gtcggcagcg ttcaatgcac aagctgaaac
      901 ctggttcgat ccagcctttt tcaaagatga tccctcaatg gtggccgatt tgtctcgttt
      961 cgaaaaagga caaaaaataa cgccaggggt ttatcgtgtc gatattgttc tgaatcagac
     1021 aattgtagat acgcgcaacg tcaattttgt tgagataacg ccagagaagg ggattgccgc
     1081 ctgtttgacg accgaaagcc tggatgcaat gggcgtgaat actgatgcat ttccggcttt
     1141 taaacaactg gacaaacaag cgtgtgcgct attggcggag attattccgg atgccagcgt
     1201 aacttttaat gtgaataaac tccgtctgga aatttcagta ccgcaaattg ccatcaaaag
     1261 taatgctcgt ggttatgtcc cccctgaacg ttgggatgaa gggatcaacg cgctattact
     1321 gggatattca tttagcgggg ctaacagtat tcatagcagc gcagacagtg attctggcga
     1381 cagttatttt ctgaatttaa acagtggcgt taatttaggc ccatggagat tgcgcaacaa
     1441 ttcaacatgg agtcgtagta gtggccaaac cgcagaatgg aagaatctca gcagctattt
     1501 gcaacgggcg gttattccac taaaaggcga actgaccgta ggtgatgatt atactgcagg
     1561 cgattttttc gatagcgcca gctttcgtgg tgtgcagctg gcatcagatg acaacatgct
     1621 gccagacagc ctgaaagggt ttgcgcctgt agtgcgtggt atcgccaaaa gcaatgccca
     1681 gataacgatt aagcaaaatg gttacaccat ttaccaaact tatgtatcgc ctggtgcttt
     1741 tgaaattagt gatctctatt ccacgtcgtc gagcggtgat ttgttggttg aaatcaaaga
     1801 agcggacggc agcgtcaata gctatagcgt gcccttttcc agcgtgccat tactccagcg
     1861 tcaggggcga atcaaatacg cggtgacact ggcgaaatac aggaccaata gtaatgaaca
     1921 gcaggagagc aaatttgccc aggtcacgtt gcagtggggc ggaccgtggg gaacgacctg
     1981 gtatggtggt ggacaatatg ctgaatatta ccgtgctgcc atgtttggtc tgggatttaa
     2041 cctcggcgat ttcggagcaa tttcgttcga tgcgacccag gcgaagagta cgctggcaga
     2101 ccaaagcgaa cataaaggtc agtcatatcg ttttctgtat gccaaaacgc tcaaccaatt
     2161 gggcactaat tttcaattga tgggctatcg ctattcgacg tcgggtttct acaccctttc
     2221 cgacaccatg tataaacata tggatggcta cgaatttaat gacggtgatg atgaagatac
     2281 gccgatgtgg tcgcgttatt acaatttgtt ttacaccaaa cgtggcaaac tgcaggtcaa
     2341 tatctcccag caattaggcg agtacggttc gttttattta agtggtagcc agcaaactta
     2401 ctggcatacc gatcaacagg atcggctatt acagtttggc tataacacgc aaattaaaga
     2461 tctctcgctg gggatctcct ggaactacag taagtcccgt ggtcaacctg atgctgatca
     2521 ggtgtttgca ctaaattttt ccctgccgct caatctgttg ctccccagaa gtaatgatag
     2581 ctataccagg aaaaaaaatt acgcctggat gacctctaac accagtatcg ataacgaagg
     2641 gcacattaca caaaacctgg gtttaacgga gacactactc gatgatggta acctgagcta
     2701 cagcgtgcaa cagggatata acagcgaggg gaaaacggct aatggtagcg ccagcataga
     2761 ctacaaaggg gcgtttgcag atgcccgagt gggctacaac tacagcgata acggcagtca
     2821 acaacaactg aactacgctc tttcaggcag tttagttgcc cattcacagg gcattaccct
     2881 ggggcaatcg ctgggggaaa ctaacgtgct gattgcagca ccaggcgcag agaatactcg
     2941 tgtggcgaac agcaccgggc tgaaaactga ctggcgcgga tataccgttg ttccttatgc
     3001 cacttcttat cgtgaaaatt gaatcgcact tgatgcggcg tcgttaaaac gtaacgtgga
     3061 tcttgaaaat gcagtagtca acgtggttcc caccaaaggg gcgttggttc tggcggagtt
     3121 caatgcccat gcgggagcaa gggtattaat gaaaacaaca aagcagggta taccgctgcg
     3181 ttttggcgcg atagcgacgc tggacggggt acaggctaat agcggcataa ttgatgatga
     3241 tggctcgctc tatatggcgg gtttaccggc gaagggaaca ataaccgtac gctggggcga
     3301 agcacccgat caaatttgtc atatcagtta cgagcttacc gaacaacaaa ttaactctgc
     3361 gattacgcga atggatgcca tatgcagata atctttggag aaaaatgcgt gtcattacta
     3421 cgactatttt ttgccgccgt cttaatgcta tggtgcgctc aaaccgctgc ttatagcggg
     3481 cagtgtcata ccactcaggg gaatccgtat attggcgtca attttggcgt taaaaccctg
     3541 gaggaagaag aaaatactgc aggggtagtt aaagacaaat tttatcagtg gaacgaatcg
     3601 aatgattatt atgtttcctg tgattgcgat aaagacaatg tcagaaatgg tcgatgggca
     3661 ttcgccgcgg attcaccgtt agtctattta ggcgacaact ggtacaaaat taatgactat
     3721 cttgccgcca aagttttatt gcaggttaaa ggcagttctc ctactgcggt tcctttcgaa
     3781 aacgtgggca caggggggga tacccgatgg catatttgcg accctggcgg tcaacgttta
     3841 ggcggccagg gggctagcgg taatagcggt agcttttccc tgaaaatatt gcagccgttc
     3901 gttggctcgg tcgtcattcc tcctatggcg ctggcgcgat tatttgaatg ctacaacata
     3961 cctgcaggtg attcctgcac gactacaggt acaccggttt tagtgtatta cctgtctggt
     4021 acgatcaatt cacttggctc atgttccgtc aatgccggag aaacaatcga ggttgatctg
     4081 ggcgacgtat ttgcggctaa ctttcgtgtt gtagggcata agcctcttgg ggccagaacg
     4141 gcagaacttg caattccggt caggtgtaac acgggaaacg cggggttagt taacgtcaac
     4201 ctgagtctga ctgcaacctc agaccccagc tatccccagg cgattaagac gtcacgtcca
     4261 ggcgtgggcg tggtggtgac cgatagccag aacaacatta tttccccggc tggtggaaca
     4321 ctaccgctct ctattcctga tgatgcagac agtatcgcgc gaataaatgt ctatccagtc
     4381 agcacgacag gtgtaccacc agaaaccggg cgatttgaag ccacggcaac ggtgagaata
     4441 aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atttcagtgc gttatcttgt
     4501 tctttagcat tcttaacatc catgtcggga tggctgggcc tgaacaagtt agtatgcata
     4561 tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca ttacaaaata
     4621 ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaatatcgta agcactgctg
     4681 ctgatttgaa tatcgatatc accggttgtg ccgctggtat tactggcgcg gacgtccttt
     4741 ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac acaggcggaa
     4801 gcggtggtat ggcaacgggg attgccgtgc aaattcttga tgcgcaaagt cagcaagaaa
     4861 tcccgctcaa tcaggtccag cctcttacgc ccttaaaagc cggggataac acactcaaat
     4921 atcaacttcg ttataagtcc acaaaggcgg gagcaacggg cggtaatgcg acggcggttc
     4981 tctattttga tctggtttac cagtgaagga gtgatgttga aacggataat ctggattctg
     5041 ttcttactgg gattaacggg gggttgtgag ctatttgccc atgatggcac ggtcaacatt
     5101 agcggatcgt ttcgccgtaa tacatgcgtg ctggcacagg atagcaagca aatcaatgtg
     5161 cagttaggcg atgtctcact gacacgtttt tctcatggga attatggccc ggagaaatct
     5221 ttcatcatta atttgcagga ttgcggaaca gatgtcagca cggttgacgt caccttttca
     5281 ggaacccccg acggagtgca gagcgaaatg ttgtccattg aaagtggtac ggatgcagcc
     5341 agcgggctgg caattgcgat tctggatgat gcgaagatat tgattccgct taaccaggcg
     5401 agtaaagact acagcctaca tagcggtaag gtgccattaa ctttttatgc gcaattgcga
     5461 cctgtcaata gcgatgtgca gtcgggtaaa gtgaatgcca gcgcgacatt tgtacttcat
     5521 tatgactaat acgtggaaca gattggcgct cttgattttc gccgttttat cgctgctggt
     5581 ggcgggtgaa ttgcaggcag gagtggtggt cggcggaacg cgatttatct ttccggcaga
     5641 cagagaatcg atatctattt tactgactaa taccagtcag gaatcctggc ttattaacag
     5701 taaaatcaac cgcccaacgc gttgggccgg gggtgaagcg tcgacagtgc cggcaccatt
     5761 actggccgct ccgccactta ttctcctgaa gcccggtacg acaggcacgt tgcgcttgct
     5821 gagaacggaa agcgacatct tgcctgtgga tcgcgaaacg ctatttgagt taagcattgc
     5881 cagcgtgcca tccggcaaag ttgaaaatca gagcgtaaaa gtggcgatgc gctcggtatt
     5941 taaactgttc tggcgacccg aaggtttgcc gggcgacccg ctggaagctt atcaacaatt
     6001 acgctggaca cggaattcgc agggtgtaca actcactaac ccaacgcctt actacattaa
     6061 cctgattcag gtaagcgtaa atggtaaagc gttaagtaat gtgggagttg tgccgcctaa
     6121 aagcctgcgt cagacaagct ggtgtcaggc aatagcaccg tgtcatgttg cctggcgggc
     6181 gataaatgat tacggcgggt tgagtgcaaa gaaggagcaa aatctgccct gaaacaggtt
     6241 cggaaaacgt ttgcgttttt tttgccgcag gtcaattccc ttttggtccg aactcgcaca
     6301 taatacgccc ccggtttgca caccgggaat ccaggagagt tcatgtacta ccccttcgtt
     6361 cgtaaagccc ttttccagct cgatccagag cgcgctcatg agtttacttt tcagcaatta
     6421 cgccgcatta ccggaacacc gtttgaagca ctggtgcggc agaaagtgcc tgcgaaacct
     6481 gttaactgca tgggcctgac gtttaaaaat ccgcttggtc tggcagccgg tcttgataaa
     6541 gacggggagt gcattgacgc gttaggcgcg atgggatttg gatcgatcga gatcggtacc
     6601 gtcacgccac gtccacagcc aggtaatgac aagccgcgtc tctttcgtct ggtagatgcc
     6661 gaaggtttga tcaaccgtat gggctttaat aatcttggcg ttgataacct cgtagagaac
     6721 gtaaaaaaag cccattatga cggcgtcctg ggtattaaca tcggcaaaaa taaagatacg
     6781 ccagtagagc agggcaaaga tgactatctg atttgtatgg aaaaaatcta tgcctatgcg
     6841 ggatatatcg ccatcaatat ttcatcgcca aataccccag gattacgcac actgcaatat
     6901 ggtgaagcgc tggatgatct cttaactgcg attaaaaata aacaaaatga tttgcaagcg
     6961 atgcaccata aatatgtgcc gatcgcagtg aagatcgcgc cggatctttc tgaagaagaa
     7021 ttgatccagg ttgccgatag tttagttcgc cataatattg atggcgtgat tgccaccaat
     7081 accacactcg atcgttctct ggttcaggga atgaaaaatt gcgatcaaac cggtggctta
     7141 agtggtcgtc cgcttcagtt aaaaagcacc gaaattattc gccgcttgtc actggaatta
     7201 aacggtcgct taccgatcat cggtgttggc ggcatcgact cggttatcgc tgcgcgtgaa
     7261 aagattgctg cgggtgcctc actggtgcaa atttattctg gttttatttt taaaggtccg
     7321 ccgctgatta aagaaatcgt tacccatatc taattatttc ttcgaattcc tctttataag
     7381 ataatcaggg ctttattttc agccctggtt gttttatatt catcactgtt gcttatttag
     7441 acattttgta cttttattgt tgaggttatt aagcgaagcg acaatggatt gtgttgctgc
     7501 ggttttataa ggttggggag aggcagatgc gaattaaacc agacgataac tggcgttggt
     7561 attacgatga agagcacgat cgaatgatgc tcgatttagc caatggtatg ctatttcgct
     7621 cacgttttgc gcgcaaaatg ttgactccag atgccttttc tcccgcaggc ttttgcgttg
     7681 acgacgccgc gctctatttc tcttttgaag aaaagtgccg cgattttaat ttatctaaag
     7741 aacaaaaagc cgaactggtg ctgaatgcac tggtagcaat tcgctatctc aaaccgcaaa
     7801 tgccgaaaag ctggcatttt gtttcccatg gtgaaatgtg ggtgcccatg ccgggggatg
     7861 cggcctgcgt ttggttgagc gacactaacg agcaagtgaa tttactggtc gttgaatctg
     7921 gcgagaatgc cgcgctatgc ctgctggcac aaccctgcgt tgttattgcg ggtcgcgcga
     7981 tgcagttggg cgacgccatt aaaatcatga acgacaggct gaaaccgcag gttaatgttg
     8041 acagcttcag cctcgaacag gcagtctaac gcgccaactt aagtgcagtc ttcggtacac
     8101 agctacagca aagaatggtg ccatcatcgc ccattgctga ttttttcagc ggcgtgactt
     8161 cgccttctaa aagctgaaca cggcaacttc cacaaatgcc cgcgcggcaa gaataaggga
     8221 tacgaattcc ctgattttct aattgctcca gcaacacctg ttggttattt ccacgaaatg
     8281 cctgtccctg ccagtcaata tctacattgg cgtccggttg ttgcgtgatg ttggcagtat
     8341 catcagcggc agctgcgccg taaatttttg ccggagccgt tgtcagaatt tccacctcat
     8401 cgccaacgcg aatcacgccg ctattacggg caattaaatt ctgaccaaaa tcgacatcgc
     8461 cgttatcctg ggcagtgcgg aaagattgca atgtttttaa tggttcgcct gccggatgtt
     8521 tttgcccttt ttctgggctg acggtggtga aaatacagcg gctacaaggt ttaaccacat
     8581 caaacaccac atcaccaatg cgaatcactt tccaactatc ttcttcccag gctgacgcgc
     8641 cggaaaccac cagattgggg cggaattgct ccatttttac actggccgga caacgttgtt
     8701 ggagatcacg taacgaggct tcgttagcaa gaaggtaagg atagccatca gcaaatgaca
     8761 ggggtacagt gttgtggcgt ttcacgcgcc gggtcatttg tggccccacc cagcgtaatt
     8821 gcacttcgcg ggagaaaaat ccacttaacc atttgttgat cgcttctggc gcaattcgcg
     8881 cggtaaaatg tgtgccccaa acttcggttg gtgcgtcttg tgtggcgaaa tcagcaaaac
     8941 gaacatatgc gctactgcca tctggtgcgg tgagatgcaa gccatcatgc acaggtgaag
     9001 gagtaaaccg taccatctgg ggaaactggc gagcggtaat aaacgtaccg tcaggttccg
     9061 tgatcataaa gatgcgatcg aaggccagac cactgacatc agccagagca tgtgtaagac
     9121 caatgccgcg catcgattta acaggatgaa taaaaagccg ggttaatgtc gccacggtgt
     9181 ggtcctcaaa tgaaaataag ccctcaactt tatgacatga cgcgcttatt agctataatg
     9241 cgcaacaatt ttcttagcgc ctgtcccatt aggctatttt atttgtcagt ttggccctgg
     9301 gttgtgctcg aaatcctcac gtacttgtgt acgctccggt ttctccgcgc tgtccatgtc
     9361 cagactgact gcaacaatta cgcctactgc ggtaggctct tagagtaaaa gtgacgatat
     9421 gaattctctg tttgccagta cggcccgtgg gctggaagag ctgttaaaaa ctgaactgga
     9481 aaacctgggg gccgttgaat gccaggtggt tcagggtggg gtccatttca agggcgacac
     9541 acggcttgtt taccagagcc tgatgtggag ccgcctggcc tcgcgtatta tgttgccgct
     9601 gggcgagtgt aaggtttaca gcgatttaga cctctatctc ggtgttcagg cgatcaactg
     9661 gacagagatg tttaatcctg gcgcgacctt cgctgtccac ttcagtggtt tgaatgacac
     9721 aatccgcaac agtcagtacg gcgcgatgaa agtgaaagac gcaatcgtcg atgctttcac
     9781 gcggaaaaat ctgccgcgtc caaatgttga tcgcgatgcg ccggatatcc gcgttaacgt
     9841 ctggctgcat aaagaaaccg ccagtatcgc tcttgatctg agtggtgatg gtttacatct
     9901 gcgtggctat cgcgatcgtg ctggtattgc gccgatcaaa gaaaccctgg cagccgctat
     9961 tgtgatgcga tccggctggc agccaggaac accgctgctc gatccgatgt gtggttccgg
    10021 tacgttgctg attgaagcgg cgatgctggc gaccgatcgc gcaccaggct tgcaccgtgg
    10081 gcgttggggc tttagcggct gggcgcagca tgatgaagct atctggcagg aagtgaaagc
    10141 agaagcgcaa actcgcgccc gtaaaggcct ggctgagtat agctctcatt tttacggttc
    10201 ggacagcgac gcacgggtga ttcaacgtgc gcgcactaac gcccgtcttg cggggattgg
    10261 tgaactgatc acctttgagg tgaaagatgt cgcgcaactg accaatccgc tgccgaaagg
    10321 gccgtacggc acagtgttga gcaacccgcc atacggggaa cgtctggaca gcgaaccggc
    10381 gctgattgcg ctgcatagcc tgctgggtcg gatcatgaaa aaccagtttg gtggctggaa
    10441 tctctctttg tttagtgcct cgccggatct gctaagctgc ttgcagctgc gtgcagacaa
    10501 acagtacaag gcgaaaaacg gcccgctgga ctgcgtacag aaaaattacc acgttgccga
    10561 aagcactcca gacagcaaac cggcgatggt agcggaagat tacgccaacc gcctgcgtaa
    10621 gaacctcaaa aaattcgaga agtgggctcg ccaggaagga attgaatgtt accgcctgta
    10681 tgacgccgat ctgccggaat ataacgttgc cgttgaccgt tatgccgact gggtggtggt
    10741 gcaggagtat gcaccgccaa aaactattga tgcgcacaaa gcgcgtcagc gtctgttcga
    10801 tattatcgct gcaaccattt cggtactggg gattgcgcca aacaaactgg tgctgaaaac
    10861 ccgtgaacgc cagaagggca aaaaccagta ccagaaactg ggcgagaagg gcgagtttct
    10921 tgaagttacc gaatataacg ctcacttgtg ggtgaacctg acggattatc tcgatactgg
    10981 tctgttcctc gatcaccgta tcgcccgccg tatgctcggt cagatgagca aaggcaaaga
    11041 tttcctcaac ctgttttctt ataccggcag cgccaccgtg catgcgggat taggcggtgc
    11101 acgcagcacc accaccgtgg atatgtcgcg tacttatctg gaatgggcag aacgcaacct
    11161 gcgtctgaat ggcctgaccg ggcgtgcgca tcgcctgatt caggccgatt gcctggcgtg
    11221 gctgcgtgag gcaaatgaac agttcgatct gatctttatc gatccgccaa ccttctctaa
    11281 ctcaaaacga atggaagatg cgtttgatgt tcagcgcgat catctggcgc tgatgaaaga
    11341 tttgaaacgt ctgctgcgtg caggtgggac gatcatgttc tcgaacaaca aacgtggctt
    11401 ccgtatggat ctcgacggcc tggcaaaact gggactgaaa gcacaagaaa ttacgcaaaa
    11461 aacgctctcc caggatttcg cccgtaaccg ccagatccac aactgctggc tgattaccgc
    11521 agcctgaaag gaatagtaat gtcattaatc agtatgcatg gcgcatggct gtcgttcagc
    11581 gacgcgccgc ttctcgataa cgcagaactg catatcgaag ataacgaacg tgtttgtctg
    11641 gtgggccgca acggcgcagg caaatcgacg ttaatgaaaa tcctcaaccg tgaacaaggg
    11701 ctggatgacg gtcgcattat ttacgagcag gatttgattg tagcgcgtct gcaacaggat
    11761 ccgccgcgta acgttgaggg tagtgtttat gatttcgttg ccgaaggcat tgaagaacaa
    11821 gcggaatacc tgaaacgcta tcacgatatt tcgcgcctgg tgatgaacga cccgagcgag
    11881 aaaaatctca acgaactggc aaaggttcag gaacagctgg atcaccacaa cctgtggcag
    11941 ctggaaaacc gcatcaacga agtgctggcg caactggggt tagatcctaa cgttgcgctg
    12001 tcgtcgcttt ccggcggctg gttgcgtaaa gcggcattag gacgcgcgct ggtgagcaat
    12061 ccgcgcgtat tgctgctgga cgaaccgact aaccatctgg atatcgaaac catcgactgg
    12121 ctggaggggt tccttaaaac cttcaatggg acgattattt tcatctccca cgaccgttcg
    12181 tttatccgca atatggcgac gcgcattgtt gatctcgatc gcggcaagct ggtgacctat
    12241 ccgggcaatt acgatcagta cctgctggaa aaagaagaag ccctgcgcgt ggaagaacta
    12301 caaaatgccg agttcgatcg caaactggcg caggaagaag tgtggattcg ccaggggatc
    12361 aaagcacgcc gtacccgtaa tgaaggccgc gtacgcgccc tgaaagcgat gcgtcgcgag
    12421 cgtggcgaac gtcgcgaagt gatgggtacc gcaaagatgc aggtggaaga ggccagccgc
    12481 tccggtaaga tcgttttcga aatggaagac gtttgctacc aggttgacgg aaagcaactg
    12541 gtgaaagatt tttctgccca ggttctacgt ggcgacaaaa ttgccctgat tgggccgaat
    12601 ggctgcggca aaaccacgct gctgaaactg atgctcggtc agcttcaagc ggacagcggg
    12661 cgtattcacg ttggcaccaa actggaagtg gcttatttcg atcagcaccg cgcggaactt
    12721 gatcccgata aaacggtgat ggataacctt gccgaaggta agcaagaggt gatggttaac
    12781 ggcaagccac gccacgtttt gggctatttg caggacttcc tgttccatcc gaaacgggcg
    12841 atgacgccag tacgtgcgct ttctggcggt gagcggaacc gcttgctgct ggcgcgtttg
    12901 ttcctcaaac caagcaactt attgattctt gacgaaccga ccaacgatct tgatgtcgaa
    12961 acgctggagc tgctggaaga actgatcgac agctaccagg gcacggtatt gctggtaagc
    13021 cacgatcgtc agtttgttga taacaccgtt acagaatgct ggatcttcga aggcggcggt
    13081 aaaattggtc gttatgtcgg cggttatcat gacgcccgtg gtcagcaaga gcagtatgtg
    13141 gcgctcaaac agcctgcggt gaaaaaaaac gaagaagccg ccgcgccaaa agcagaaact
    13201 gtaaaacgca gcagtagcaa actaagctat aaattgcagc gcgaactgga gcagctaccg
    13261 caattgctcg aagatctgga ggcgaagctg gaagccctac agacgcaggt ggcggatgct
    13321 tccttcttca gtcagccgca tgagcagacg caaaaagtgc tcgctgatat ggctgccgca
    13381 gaacaggaac tggagcaagc ctttgaacgc tgggagtatc ttgaagcgtt aaaaaatggt
    13441 ggctgatcgc aaaatagtcg atttaaacag agacagctga caacggtaga attgcctgat
    13501 gcactgggtt tatcaggcat gaaaaccaac actttgttag tcatctgtaa aatagcgcat
    13561 cattaaggag taccaatgtg cgaacatcat catgccgcga agcacatcct gtgctcgcag
    13621 tgtgacatgc tggtagcgtt accgcgcctt gagcatggtc agaaagcggc atgtccccgg
    13681 tgtggcacaa cgttaaccgt ggcgtgggat gccccccggc agcgtcctac cgcctatgcg
    13741 ttggctgcac tgttcatgct gttgctgtcc aacttgtttc cttttgtgaa tatgaacgtt
    13801 gcaggagtta ccagtgaaat tacattactg gaaattcccg gcgtgctttt ttctgaggac
    13861 tacgccagcc tcggcacctt tttcctgttg tttgtgcaac tggttcccgc gttttgtctg
    13921 ataaccattc tgttactggt gaatcgcgcg gaattaccgg ttcgtttaaa agagcaactg
    13981 gcacgggtgc tttttcaact caaaacctgg ggaatggcgg agattttcct cgcgggtgtg
    14041 ctggtcagtt tcgttaaact gatggcttac ggcagcattg gcgtaggcag cagctttctc
    14101 ccctggtgtt tattttgtgt cctgcaactg cgtgcttttc agtgcgttga tcgtcgctgg
    14161 ttatgggacg atatcgcccc gatgccagaa ctgcgccagc cgctaaaacc aggcgtcacg
    14221 gggatacgtc aggggctgcg ttcgtgctcc tgttgtacgg caatccttcc tgctgatgaa
    14281 cccgtgtgcc cgcgctgtgg tactaaaggg tacgttcgac gtagaaacag cctgcaatgg
    14341 acactcgcgc tgcttgttac gtccatcatg ctgtatcttc cggctaatat tttgcccatc
    14401 atggtgacgg atttattagg ctcgaagatg ccatcgacga ttctcgctgg ggtcatcctg
    14461 ttatggagcg aaggctctta tcccgtcgct gcggtgatct tccttgccag tattatggtg
    14521 ccaacgttaa agatgatcgc catcgcgtgg ctgtgttggg atgccaaagg gcatggcaag
    14581 cgcgacagtg aaagaatgca tttgatttat gaagttgttg agtttgtagg ccgctggtcg
    14641 atgattgacg ttttcgttat cgcggtgctc tcggcgctgg tgcgtatggg aggtttaatg
    14701 agtatttatc cggcaatggg tgcattaatg tttgctttag tcgtcataat gacaatgttt
    14761 tctgctatga cgtttgaccc gcgtttgtcg tgggatcgtc aacctgaatc agagcatgag
    14821 gagtcctgac agtatggaat ctaataatgg ggaagccaaa atccagaaag tgaagaactg
    14881 gtctcccgtg tggatatttc ctatcgtcac ggcgctcatt ggggcctggg ttctttttta
    14941 tcattacagc catcagggac cggaagtgac cctgatcacc gcgaatgcgg aaggaattga
    15001 aggtggcaaa accaccatta aaagccgtag cgttgacgtc ggcgtggttg aaagcgccac
    15061 actcgctgat gatttgacac acgttgaaat caaagcgcgg ctgaattccg gtatggaaaa
    15121 attgctgcat aaagataccg tcttttgggt ggtgaaaccg cagattggtc gagaagggat
    15181 tagcggtctg ggaaccctgc tgtctggggt ttatatcgaa ctgcagccag gcgcgaaagg
    15241 cagcaaaatg gataaatacg atttgctgga ctcgccaccg ttggccccgc ctgatgcgaa
    15301 aggtatccgt gtgattctcg atagcaaaaa agccgggcag ctctcgccag gagatccggt
    15361 gctgttccgt ggctatcggg taggttcggt tgaaaccagc accttcgata cgcaaaaacg
    15421 caatatcagt tatcaactgt tcatcaatgc accttatgac cgactggtga ccagcaatgt
    15481 tcgcttctgg aaagatagtg gcattgcggt tgatctgacg tcagcgggaa tgcgtgtgga
    15541 gatgggctca ttgacaacgc tgctgagtgg cggtgtcagc tttgatgtgc cggaaggtct
    15601 ggatttaggg cagccagtgg caccgaaaac agctttcgtt ttgtatgatg atcagaagag
    15661 cattcaggat tcgttgtaca ccgatcacat tgattatctg atgttcttta aagattcggt
    15721 acgcggtctg caaccgggag ctccggtaga attccggggt attcgcctcg gtaccgtaag
    15781 caaagtgcca ttctttgcgc cgaatatgcg tcagacattt aacgatgatt accgtattcc
    15841 ggtgctgatt cgtatcgagc cagagcggct gaaaatgcag cttggcgaaa atgcggatgt
    15901 tgttgagcac cttggcgaat tgttgaaacg tggtttacgc ggatcgctga aaaccggaaa
    15961 cctggtcact ggcgcactgt atgttgatct cgatttctat ccaaatacgc ctgcaataac
    16021 cggtattcgt gaatttaatg gttatcagat tatcccgacc gttagcggcg gcctggcgca
    16081 aatccagcaa cgactgatgg aagcgttgga taagatcaac aaactgccat tgaatccgat
    16141 gattgaacag gcaaccagta cgctttctga aagtcagcgc acaatgaaaa acctgcaaac
    16201 gacgctggat agcatgaaca agatcctcgc cagccagtcg atgcagcagt tgccgacgga
    16261 tatgcagtca acgttgcgtg aattgaatcg cagcatgcag ggcttccagc ccggctccgc
    16321 agcctacaac aagatggtgg cggatatgca gcgccttgat caggtgttgc gagaactgca
    16381 accggtgctg aaaacgctca atgagaagag taacgcgctg gtatttgaag cgaaggacaa
    16441 aaaagatcca gagccgaaga gggcgaaaca atgaaaaagt ggctagtgac gattgcagca
    16501 ctgtggctgg ccggatgcag ctccggcgaa attaataaaa actattacca gttacctgtg
    16561 gtgcagagcg gtacacaaag taccgccagc cagggcaatc gcctgttatg ggtagagcag
    16621 gtcgctgtac ctgactatct ggcggggaat ggtgtggttt atcaaaccag tgatgtgaag
    16681 tatgtgattg ccaacaacaa cttgtgggcc agcccgttgg atcaacagtt gcgcaacacc
    16741 ctggtagcca acctgagcac gcaactgccc ggctgggtgg ttgcctccca gcctctggga
    16801 agcgcccagg acacgctcaa tgttaccgta acggagttta acggtcgcta tgatggcaag
    16861 gtcattgtca gtggtgagtg gctgttgaac caccagggac aactgatcaa acgtccgttc
    16921 cgtctggaag gagtgcaaac tcaggatggt tacgatgaga tggttaaagt gctggccggt
    16981 gtctggagtc aggaagccgc ttctattgca caagagataa agcgtctacc ttaattataa
    17041 agatttgtaa atataaccgt ctccggtatg ttgcctgagg cggttttttt gtttctaacg
    17101 tgcggaaaaa tttgttcctc ttcacatttt ttgtacaacc gacatgcccg tgtagctcac
    17161 aaatatgaca gtggcgtgaa ttttgcgcat tgacggcagt tatgattcgc ggtattgctt
    17221 aactgtgatt gcacatttag taatcactgt tttcttttcc accagaaacc agtatgaggg
    17281 aaacgaggca tgaagagaca aaaacgagat cgcctggaac gggcacatca acgtggttat
    17341 caggccggca tcgccggacg ctcaaaagaa atgtgtccct atcagacgct gaatcaaagg
    17401 tcacaatggc tgggaggctg gcgagaagcc atggcggaca gggtagtaat ggcctgattc
    17461 tgtctcttta aaaagaaacc tccgcattgc ggaggtttcg ccttttgata ctctgtctga
    17521 tgataatcag aaggcagacg tatcctggaa cagaccgact ttcaggtcgc tggcggtata
    17581 gatcagacga ccatcaacca gcacttcgcc atctgccaga cccataatca gacgacgatt
    17641 aacaatgcgt ttaaagtgaa tacggtaggt cacttttttc gctgtcggca gtacctgacc
    17701 agtgaatttc acttcgccaa cgcccagcgc gcggccttta ccttcgccgc ccagccagcc
    17761 gaggtagaac cctaccagct gccacattgc gtccaggccc aggcatcccg gcataaccgg
    17821 atcgccaata aagtggcatc cgaagaacca cagatccgga ttgatatcca gttctgcttc
    17881 aacataccct ttgttgaagt taccacccgt ttcggtcatt ttgaccacac ggtccatcat
    17941 cagcatgttc ggtgctggca attgcgggcc tttagcgcca aacagttcac cgcgaccaga
    18001 ggcaagaagg tcttcttttg tataggattc gcgtttatct accatgttct ctgtaagcct
    18061 tattttattg aagcacgcag gatagctaac acgtgtacgc tgaacaagtc cgatcagttc
    18121 ggaataaacc agttcagcca acgtaatggc catggaaaac ggtgacgtcc ttcctgttgc
    18181 gatgcttgcg cgatacgttc ctggatggtt tgcatcagcg tcgtttggcc ttcgccatcc
    18241 cacaccagat ttaataataa cggcagtgcg tcagtcacat cgtctactgc ccagatggtg
    18301 aatttgcctt cttctaccgc tttcaccagt tcactgtgaa gacttaaatg gcgaacgtta
    18361 gcagtgggga taatgacacc ttgtttaccc gttaactcac gttgctggca aatagcaaag
    18421 aagccttcga ttttctcatt taaaccaccg accggctggg cgcgaccgaa ctgatcgact
    18481 gaacctgtga tagcgatact ctgattcacc ggcacatcgg cgagggcgct tatcagagcg
    18541 cagagttcag ccatcgaggc actatcgcca tccacttcac tgtatgactg ctcaaatgtc
    18601 agcgatgctg agaaggggat ctgttgctca agctgtagtt ccgacatcag gaacgcctgc
    18661 atgatcatca tccctttcgc atggatattg ccgccaagct ccgctttgcg ttcgatgtcg
    18721 gtgaattcac catcgccaat atgcacaacg cagctaatgc gagaaggttc gccaaaagcg
    18781 cgtggatgac ccggaaattc aatgaccgaa agggcgttaa tttgcccgat gcgttcgcct
    18841 tcggtttcaa tcaggatttg ctcctgaagg atctcatcct gcatgcgttc agcgaggaaa
    18901 ccttcgcgcc attcacgctg ctgtagcatt aagtttagct gctcgccgga gaaggtgtcg
    18961 ccatcacaca gggaggcgac ttctttacac tggcggagga tccactgcgg gctaagcgga
    19021 agtgtttctt gttcaccggt gtagcgtgct gcttcgcgga taagtatcgg ccaggcatcc
    19081 gctcccggtg caggtaagtg attatgtctg gcggtaaatg tcacccagcg acaccactgg
    19141 cttactgact ccgcatcgac aatctggaga gtatcttcaa attcgctata aatagcctgc
    19201 tctgaaagct ctggctccat ctcctggaaa tcagccaatg attcgcgttc gcctaccaga
    19261 atgactttca gcttcaatgg catcgaaggc acagagacgg ggagagggcg cgactcatca
    19321 aacgcaaccc agtcaaaacg ctcgcggtta acgatatttt tcagccgcat ccacagcaga
    19381 ggttgcgcca gcagtgtacg caaagagatg atgagaatac cgccatttgc ctgatgcacc
    19441 agaccaggct gcagggtaat gtcgccatta aactggcgca ggcagccaaa gagttgctcc
    19501 gcttctaccc agtcggcagc gacaacttgc gttaaagtcg caaaattatc atctgcactc
    19561 actgcgttac gtaagcggat ggtgtggccg gaaacctcat agtgaccgcc caccagttgg
    19621 cctgcatcac tttgtagcgc acgtgccgca tcggcaataa gattcagata ctcagactct
    19681 tccggggcct tcgccagcat aaaagaggag gatgctcgcg tatgcagcaa ctgctccagc
    19741 gcaaattgca gtcgcggttg agtatcactg aataaaggat cgttttcgtc aatcaaatgt
    19801 ggctgagcaa atatttcctg atagctatcg gtatcaggaa ccaggtcacg ccatgcaagt
    19861 ttcgtaatgg tcaaagttga tgttttttag tctgttgtca aagccgcgat tataccgtaa
    19921 ccggcactac agcacacgta gaaagcaccg acaatactcc tggcatgggc gttaaagctc
    19981 acaggatgga gattcttttc ttcactggcc taaaaagctg atattctgta aagagttaca
    20041 cggtaacatt gagatcgcta tgaaatatca acaacttgaa aatcttgaaa gcggttggaa
    20101 atggaagtat ctggtgaaga agcatcgcga aggggagtta attacccgtt acatagaagc
    20161 cagtgccgcc caggaagccg tcgatgtgtt gctctcgctg gaaaatgaac ccgtgctggt
    20221 aaatggctgg attgacaagc atatgaatcc ggaactggtc aatcgcatga agcaaaccat
    20281 tcgggccaga cggaagcgcc attttaatgc ggaacaccag catacgcgca aaaaatcgat
    20341 cgatctggaa tttattgtct ggcaacgtct ggctggtctt gcgcagcgtc gcggtaaaac
    20401 actttctgaa acgattgttc agctgattga agatgcggaa aacaaagaga aatacgcgaa
    20461 taaaatgtct tctttgaagc aggatctgca ggcattgctg ggtaaggaat aactgatgaa
    20521 actcagttca atttactaaa ggcgaaaaaa aaccccgcag ctgcggggtt ttttaccaga
    20581 cgagaactta agcctgcggc tgagttacaa cgtctttgat acctttaact tcgatctcaa
    20641 cgcgacgatc cggagccagg cagtcgatca gtgcagcacg ctgtttcacg ttgtcacagg
    20701 tgttgccagt aaccgggttg gattcgccca taccacgtgc ggagatcttg tctgccggga
    20761 tacctttgga gatcaggtaa tcaacaacag actgagcacg gcgctcggac agaccctggt
    20821 tgtaagcgtc agaaccgatg cggtcggtgt aacccagaac aactacggaa ccgtctttcg
    20881 gatccaggtt gctcagctgg ctgtacagct gatccagagc agcctgacct tccggtttca
    20941 gggttgcttt gttgaagttg aacagaacgt cagacttcag agtgaagtgc ttggtctgta
    21001 cttccggtgc cggagctgga gccggagcaa ctaccggagc tgcttcgccc tgaccgaaac
    21061 ggtaggaaac acccaggctc agcaggccgt tgtccggacg agtgccgatg gtgtgtgcgt
    21121 caccgatgtt gttggtccac tggtattcca gacgggtagc gatttcagga gtgatggccc
    21181 attcaacacc acctgcgaat accggagaaa cgccggtatc gtggtttttc tcagattcac
    21241 ctgtcacatt gttgtgagct ttggtgtctg cacgccaaac cataccaccc agacgagtgt
    21301 acacgtccag gtcgtcagtg attgggtaac ccagtttagc ggtcaactga acgccctgag
    21361 ctttgtatgc accgttttca acgctgcctt tgtacggcat acgacctaac cagtcgtaac
    21421 ccatttcaaa gccaacatac gggttaacct ggtaaccacc aaaagcacca gcgcccagtt
    21481 ggttttcatg ggtcgggcca ttgttgtcga tgaaaccagt gtcatggtac tgggaccagc
    21541 ccagtttagc accagtgtac caggtgttat ctttcggagc ggcctgcgct acggtagcga
    21601 aaccagccag tgccactgca atcgcgatag ctgtcttttt cattttttgc gcctcgttat
    21661 catccaaaat acgccatgaa tatctccaac gagataacac ggttaaatcc ttcaccgggg
    21721 gatctgctca atattaactc taccgatatc ttcggcttat gccgagcacc cctggcgatg
    21781 taaagtctac aacgtagttg aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa
    21841 aagtcttgta taaggtatgt ttaatctttt ttgtcagcga caatttacag aagaaaatcg
    21901 cggaaaccgc ttcagacaag cctccgcaag gaaaattaat cacgactgaa agcattggct
    21961 ggacgacaaa aaaagttcca ggattaatcc taaatttact taatgataca aattagagtg
    22021 aatttttagc ccggaaagtt gtctcgttgc gtgagaggat gcgcttaccg gacgcataat
    22081 aaaccccata gcgttacctt catttgccgc atcaacaagt tcagcatgct cttcttcagt
    22141 caaatcatct gccaaccaac cgatcaccac actgtaattg cccgtgcgta aagcgcgaac
    22201 cattgactcc acagtgtggc aaggggggag ctggctaatc tgcattactt tcgttaaggg
    22261 tagcccagat gcctgaaccc attcccgact cagtttttgt tgcggtgtta accagagttg
    22321 ccagcgcgat tgctgaccga gttgctgtaa caatggcaac agtagaagtt gcgtcatcat
    22381 gggctgatct tcgcgataga caacttcact gataagcccg gctgtagtgt tttccgtaga
    22441 gacacgcgca attttacttg ctgcggatga gaacgacgaa gaacgatgtg catagcctga
    22501 agtgtacata atcaatccag cccctgtgag ttactgtatg gatgtacagt acatccagtg
    22561 acgacaaaga tcaaccctat tttcggaaag agcctcgcaa attttgtcgt tggtgacggg
    22621 aaaacataaa ttaatcttgc ccctaaagaa taagttgcct attttcgtat ttaacggatc
    22681 cgttaatgtg aaccattctt ttatgttatg attttaaaag gaattttatg aaaagcctct
    22741 cctataagcg gatctataaa tcacaagaat acctggcaac gttgggcaca attgaatacc
    22801 gatcattgtt tggtagttac agcctgaccg ttgacgacac ggtgtttgcg atggtttctg
    22861 atggtgagtt atatcttcgg gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc
    22921 ctgtctggct gacatataaa aagtgtggcc gatccgttac cctcaattac tatcgggttg
    22981 atgaaagtct atggcgaaat caactgaagc tggtgcgtct gtcgaaatat tctctcgatg
    23041 cagcgctgaa agagaaaagc acgcgcaata cccgggaaag actgaaagat ttgcccaata
    23101 tgtcttttca tctggaagcg attctcgggg aggtggggat taaggatgta cgggcgttac
    23161 gtatacttgg ggcaaaaatg tgttggttgc gactgcggca gcaaaacagt ctggtgacag
    23221 aaaagattct gtttatgctt gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg
    23281 tggcacgccg ccaggagctt gcagaatggg ctgactctct tacgccgaaa caggagtttc
    23341 ctgcggaact tgagtaatct ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa
    23401 taacccaact tgttgtacga ccagaggttc tttgctgtct gcccgtggtt caagttgctg
    23461 catccgctgt ttcaggccag ctaatgcctg attgacgcgt tcttcatcag caggttgatg
    23521 atgtaacgcg tcatcaacat agcaaactgc gtcatcaaga aatgccagaa tttcaggatt
    23581 agttaactgc tcccggtgag caccgagggc tgagatatag ctggtaaacg tatggttaag
    23641 gcacagcaac cgaaacgcgg cttcgcgaat ttgcggggta acgttcggtt cgctggacat
    23701 atttgatacc accgacgcca gttcagcatc acggttgtgt gcatcgcggc gggcaatacg
    23761 atacgccaga cggttatcac gcccctgatg gtattgctcc agtatggcat cgagatagcg
    23821 acagttggcc tctgtggcgc gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg
    23881 ccagatgtag ctcactgccg cccacgcaat ggcacaacca atcagcgtat cgattacgcg
    23941 aggtaacgct acttcaaaac cttcacccag taagttaaaa cacagtagca ccaaaagtgt
    24001 gatgaacatc gttgcatgag cgtattgcac gttacggaag gcaaaaaaga gcacgccggt
    24061 aataaccagc agcaccagct gcccttccag tgatggcaca aaccacagca caggaatgcc
    24121 aatggcgata cctaccagcg taccaataat ccttaacttc aggcggtggc gcgtggcgtt
    24181 atagtttggc tggcagacaa acaaacttgt cagcaagatc caatacccgt gatgcattcc
    24241 ggttatttga atgatggcgt agccgaagca caacaccagc gacattctta ccgcatgacg
    24301 gaagagggcg gattccggcg tgaagtgacg gctaagacgc agccagatat cactcaatcc
    24361 gtgcgggctg tcatcagcga gctcattttc gtcattatta tggggcagtg cctgggcctg
    24421 ttctgattca attgttgcca gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc
    24481 cagtgttttg agtaaatcgg caggcgcgcc gttatcgcgc atccgctcca gggcagcatc
    24541 aatatgcgta aaagcgcgct caaaatgcgg atcatgttga taaggctgac gcagcaaaat
    24601 acagcgtgac agttgctggc acgcctggcc ctgcatcgac atcagccgct gaaaacggaa
    24661 cagcacgtcg ctgtggcgaa aatgttcacg caatgtttga tactgaatat gagaagagct
    24721 ggcacgctcg tgaatatcct gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg
    24781 ttgaccacga tcgccacgta agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc
    24841 catcagctga ccgttggcga gagccaaatc gtacagcggt gcctggcttt catcttcaat
    24901 atcaggatca aacatgcgcg acttgagctc aagataacgc gccagttgtt catagcaacg
    24961 cgccaggttg tcctgcagcg ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac
    25021 gttgtaccag acggcaccgg ccagcagata catcggctgc tgataccagt gctcatacag
    25081 tgatgttccc aacatagtgt aaatagcgat cagcaatgca ccgaaggcaa ttgttgcata
    25141 gcgttgaccc agaccgccga gcaaaatgaa gccgctggta gagagcgtta agccaatcgc
    25201 aaatagccag ggccagggaa acagcaattc tactgaggcc gaggcgataa aaaagcagaa
    25261 cagcgtaatg atgaggttac gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc
    25321 cgctgccacc atccccagcg tcagcggaat cgtcagtttt acatcaccca gccaccacgg
    25381 aaacgctgtg gttccacaaa gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt
    25441 ccaggtatag cgtttgagca aaggacttag catataggcc atattagtgg attattcaaa
    25501 acgacgacgt gcattggctt cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg
    25561 accaaccggc catagtgcaa tagcggcaat tttaaagttc gcaataccga caggaatgcc
    25621 aatgatggaa atacattgtg cgatgcccgt tgcaatgtgc atcaggcata accaccagcc
    25681 aaagaaaatc agccagaaaa tattcaatac cgtaccgcca gtattcagca gcacattttt
    25741 gccagccggg ttcagttcat cgacatgaat agcttcattg ccataaggca ccagagacag
    25801 tttagtgatc tcccagcagg atcgtgtcag cggtaaggta aaaatcagca caatactgac
    25861 cagagtcgcc aacagccagc ccagagtggt ggcaaatccg ccaagcacaa agtttagaat
    25921 gttcaaaacg gtacgcataa aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt
    25981 gtaacgtttt tttgggctgg agcacgtttt ctctgacggt tacactgata agaaataatt
    26041 tcgtgtggat ctacagagtc atggaactga aagcgacaac gcttggaaaa cgtctggcac
    26101 agcaccctta cgatcgggcg gtgatcctca atgccgggat taaagtctcc ggcgatcgcc
    26161 acgaatacct tattcctttc aatcaattac tggcgattca ctgtaagcgc ggtctggtat
    26221 ggggcgagct ggaatttgta ctgccggacg aaaaagtggt gcgtctgcac ggtaccgaat
    26281 ggggcgagac gcagcgtttt taccatcatc ttgatgctca ctggcggcgg tggagtggcg
    26341 agatgagcga aattgcgtct ggtgttttac gccagcaact ggatttgatt gccacgcgca
    26401 ctggagaaaa taaatggctg acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc
    26461 aggctttgtc ggcgttgccg ttgccggtta accgactgga agaattcgat aactgccgtg
    26521 aggcgtggcg taaatgtcag gcctggttga aagatattga aagcgctcgg ttgcagcata
    26581 accaggcgta taccgaagcc atgcttaccg agtatgcgga ttttttccgc caggtcgagt
    26641 cttcaccgct gaatccggcg caggcccggg cagtcgttaa tggcgagcat tctctgttag
    26701 tgctggcagg tgcaggaagc ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc
    26761 tggcgcgtgg tgaagcgtcc cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg
    26821 ctgaagagat ggacgagcgt attcgcgaac ggctacatac cgaagacatt accgcacgca
    26881 cgtttcatgc gctggcgctg catattattc agcagggcag caaaaaagtt ccgatagtca
    26941 gcaaactgga aaatgatacc gctgcccgtc atgaactctt tattgctgag tggcgcaagc
    27001 aatgcagcga aaagaaagcg caggcgaagg gctggcggca atggctgacg gaagaaatgc
    27061 agtggtcagt gccagaaggt aacttctggg atgatgaaaa attacagcgt cgtcttgcct
    27121 cacgcctcga tcgttgggta agtctgatgc ggatgcacgg tggtgcacag gcagaaatga
    27181 ttgccagtgc acccgaagag attcgcgatc tgttcagtaa acgtatcaag ttgatggccc
    27241 cgttattaaa agcctggaaa ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc
    27301 ttattcatca ggcgattgtg attctggaga aaggtcgctt tatcagcccg tggaagcata
    27361 ttctggttga tgaatttcag gatatctcgc cgcagcgggc agcgttgtta gcggcattac
    27421 gcaagcaaaa cagtcagacg acgttgttcg ccgttggtga tgactggcag gcgatttacc
    27481 gattcagcgg tgcgcaaatg tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg
    27541 aacgctgtga tttagacacg acttaccgtt ttaacagtcg tatcggtgag gtggcaaacc
    27601 ggtttattca gcagaaccca ggccagctga aaaagccgct aaacagctta accaatggag
    27661 acaaaaaagc cgtcacgtta ttggatgaga gtcaacttga cgctttgctg gataagctct
    27721 ctggttatgc caaaccggaa gagcgcattc tgatcctggc gcgttaccat cacatgaggc
    27781 ctgccagcct ggaaaaagcg gcaacacgct ggccgaagtt gcaaatcgac tttatgacca
    27841 ttcatgccag caaagggcaa caggcggatt acgtcatcat cgttggcttg caggagggaa
    27901 gtgatggttt tccggctgcg gcgcgggagt cgattatgga agaggcgcta ctgccaccgg
    27961 ttgaggattt cccggacgct gaagaacggc ggttaatgta cgtggcgctg acccgggcac
    28021 gccatcgggt atgggcactg tttaacaaag agaatccctc tccctttgtg gaaatactga
    28081 aaaatctgga tgtgccggtg gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt
    28141 gcaatattac ttcagacggt ccgcgagata acgctgataa tcggggatca gaatatcgac
    28201 cgcgtcgttg aaatgcggcg actggataat gaagtctgcc gttgccacgt tggtggcgac
    28261 cggaatgttc cataccgtcg ccagacgcag caaggctttc acgtcaggat cgtgcggcac
    28321 ggcatttagt ggatcccaga agaaaatcaa tacatcaatt ttcccttctg agatcaatgc
    28381 gccaacctgc tggtcacccc ccattgggcc actcaacatc gcgttgacgt tcatgccggt
    28441 tgcgcgggaa atcaagttac cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa
    28501 cggttgatgt cgttccaccc agctcatcag catttgtttg cagtgatcgt gtgctaccag
    28561 cgcaatatgt ttccgcgcag gtaaagtgcg agtcgtcagt tccataatgt acatccgtag
    28621 ttaactttcc tacagattac tgtaagcact tatcgctgca agataaagac cgaaaaagcc
    28681 tgcgcacagg cacaaaaatc tcaggaagat ggttgttttt ccgcccattg caggaaagta
    28741 tttcgcgttt gtgggtcagc cagtttaaac caatacttca gccgttgttc tgtgagcacc
    28801 tgagactgcg gtggaatagc atccagctcg gaaacacccg aaagcagcgt actgtcatct
    28861 gccatcatcg tggcaaattg cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca
    28921 acctcgacct cataatcaat cgtttttgcc gttgaggtaa tggcgagaat atccagcttt
    28981 accggaatcg gcgtcgcatc gccatccaac aattcaaggc gcggcgcggc atcaaaatgg
    29041 ttagcttccc gctcattctc caggcgaggc aggcgaaaat tgacctggtt gatgagctgg
    29101 gtattaaaac tgaccaccag cggtggggag atatacagcc gttcttcact attggaaaga
    29161 tgaatcgtct tctcaacgcg aaacactaac tgatgcggtc cattatccag ttcaatgctg
    29221 tccgcaccgc gcagcagaga gctggaaacc ttcttgccgt cgagtaccag aagatcgaca
    29281 tcggttgaaa gccgcagcgt ggtggcaaaa acggataccg gcaaacataa tgcaatcaag
    29341 gtggtcacga tgccggtttt cataggtagc tcctgtcaaa agaccgcccg aataataatg
    29401 tgtcaaaatt tttctttaat catacttact aacatataga catatttccc gcttttgctc
    29461 tcattcattc gtattagctg catggttggc atgtcggctt tgtcgtacac tttgtgaaac
    29521 agtctggagg aaataatgaa agaaaccgat attgcaggca ttttaacgtc cactcatacc
    29581 attgcgctgg tgggggcaag cgacaaaccc gatcgtccca gctatcgcgt gatgaaatat
    29641 ctactcgacc aggggtatca cgtcattccg gtttcgccaa aagttgccgg caaaacgctg
    29701 ctaggccagc aggggtatgg cacgctggca gacgttccgg aaaaggtcga tatggtcgat
    29761 gttttccgta attcagaagc ggcgtggggc gtggcacagg aagctattgc cattggcgcg
    29821 aaaaccctct ggatgcaact gggggtgatt aatgagcaag cggcagtact ggcacgggat
    29881 gccgggttaa atgtagtgat ggatcgctgc ccggctattg agatccctcg cctgggcctg
    29941 gccaaataaa aaatccccgg aaggcaaaaa ccttccgggg atttgttcag ggattagtta
    30001 cgcagacgcg gggcctggag ttgtttgcgg atggtctgcg ccagctcatc catggatggc
    30061 tgttcaggat gttcatcctg caattcgcta cttagctggg cttcagccag gtaggtatga
    30121 accggtaggc cgttatcgtc ctccatcacc acgtgatacc acggagcggc gcgaagctcg
    30181 tcattcaccg ccagctcatc aggcgacggt tcagagagcg aataaaccgg gtcgatatcc
    30241 acgaccactc cgagataacc taacagggaa tggcggacct gctggccgat accgaatttg
    30301 ctggcaatca tagtcacctc ccgggaaatc tttacctttg atataagggt aaaattccac
    30361 atttcaagtt acatgacgcg acaggcaaac cctttcagat atagcccttc cggataggta
    30421 gcgatcaccg gatgatcggc tgcctgacgg aactgctcta taaattgtac atcacggccg
    30481 gcatcaattg cggcatccgc gatgattttc tgaaataaat cgctggtcat cagaccggaa
    30541 caggagaaag tcaggagaat accgccttca ttcagcaact gaatcgccag catgttgatg
    30601 tctttatagc cacgacacgc gcccatcaac tggcttttat tctcaacaaa cttcggcggg
    30661 tccatcacga taacgtcaaa tttttcaccg cgatcgcgat aagtacgcag caatttaaag
    30721 acatcatcac ggacaaactc agccttgctc agatccagtt tgttcagctc aacgttctgc
    30781 cgtgcaatat ccagggcttc ctgggaggta tcaacgctga caacctggtt gcaaccgccc
    30841 atcagtgccg atacggcgaa accaccggta taggagaaac agttcagtac gcgtttattt
    30901 tcaacgtagc ggcgggtagc caggcggctg tcacgctggt ccaggtagta gcccgttttg
    30961 tgtccgtgct gaatatctac cagcagcttc atgccatgtt cttcaatcgg cagcagggca
    31021 ggcggcaact cgccggtgac gaggccctgg gtcagctcca tcccttcttt tttacgtacc
    31081 gcaacatcgc tgcgatcgta aatcgcacat tccgggtaca gcgtttgcag ggcgctaatt
    31141 aatgccgcgc gctgatactc tgccccagca ctcagcagtt gcagcaccag aaaattaccg
    31201 aaacgatcga tagtaatacc cggcaggcca tcagattctc cggcgattaa acgatagctg
    31261 tcgaggccat ctttttgcgc cagccagtca cgccattttt gtgcttgttg caaacggcgg
    31321 gaaaaaaaag caatgtcgat agactcagac gggtcaaacg tccagacgcg cgcccggatt
    31381 tgcgaagctg gcgaataagc gccgcgtgct aaccattttc cctgatgatc aacaatatcg
    31441 atggtttcac cgaggctggc tttaccttcc atacgggcaa cggccccgga aaagacccac
    31501 ggatggcgac gaagtaatga tttttcgcgc cctttggcta acactaaacg tacactcata
    31561 atttactttt ctgtcgatgc aaagaaatgg gcgtcattgt ccggagtttt ccatggattt
    31621 gcaacgcgtc cattaaggat aaggagagca gcaatgtcga aagtctgcat aattgcctgg
    31681 gtttacgggc gagttcaggg cgtaggattt cgctacacca cacagtacga agcgaaaaga
    31741 ctgggcttaa ccgggtacgc caaaaatctt gacgatggca gcgtggaagt ggttgcctgc
    31801 ggtgaagaag ggcaggtaga aaaattaatg cagtggttaa agagtggcgg cccgcgttct
    31861 gcgcgtgtag aacgggtgct cagcgagccg catcatccct cgggggaatt aaccgatttt
    31921 cgtatccgtt aaatacattt taccggtttt ggcaggccag caattttggt ggcttgctta
    31981 gccggacctt tcggaaacag tcggtataaa tagcggctgt tacctttttc ttcgccaaat
    32041 ttattcgcca tcgcttttac cagcatacga atcgccggag aagtattgaa ttccagatag
    32101 aaatcacgca caaaacgcac cacttcccag tgttctggcg acagcgaaat cccttcgttc
    32161 tctgcaatca ccactgccag cggctcactc cactggctgc tttctttgag atagccttcg
    32221 gtatccgttt ctatctcttt accttcgaag atcagcataa ttattactac cttaatcaga
    32281 ctgccggcag tgtaacaaaa aacaaagccc cgcataaagc gaggctatga aagtgttagc
    32341 gggtgagatt aatcgcggct ggcgaagccc agaatgctca gcaggctgac gaagatgttg
    32401 tacagcgaaa catacaggct aaccgtggca cgaatatagt tcgtttcacc gccatgaatg
    32461 atgttgctgg tttcaaacaa aatagcgcca gaggagatca aaatgaagac cgcgctgatc
    32521 gccagatgca gagcaggcag ttgcaggaag atattagcaa ccataccaat cagcaccacc
    32581 acaatacccg ccatcagcat accgccgagg aacgacatat ctttgcgggt ggtcagcaca
    32641 tatgcagagc aacagaagaa cactaacgcc gttccgccca gtgccatagc gattacgtca
    32701 cccattccgg cagacagata ggtattcaga attggtccga ggatataacc cagaaaaccg
    32761 gtaaaggcga atgcggagat aatcccggtc ggcttattcg ccgttttata ggtcaggaac
    32821 atcaaaccat acatacccac cagcgtcaga atcagacccg gagatggcag catcagcacc
    32881 gtactggcag ttgcggtaat cgccgaaaag gccagcgtca ggctcagcag aaaataggta
    32941 ttacgcagca ccttatgggt gctaagcagt gatgtacggt catgtgaaga actaacaata
    33001 cgatccattg agtcactctc tatgacagat gtaattaatt aagcagcata atgataatgc
    33061 gtaagggcac ccagaagttt tacccatctt tacgcatttg atctggaaca ggtttaacag
    33121 cggattatca ggtcattaag caaatataac gccctgagaa tttcgacagg caaaagaaaa
    33181 aggggttagc atttagctaa ccccttatct tatctggcgg aagcgcagag attcgaactc
    33241 tggaaccctt tcgggtcgcc ggttttcaag accgatgaaa aataaataaa aatcaatgca
    33301 atactaaaaa cagctgaaaa attaaccaaa cttaacacct agaaaaatca ggctgttagg
    33361 gagctttcag agttagtata ccaagtcttt tttgccagaa aatttcctga gttgcctact
    33421 tgctgaccac aagtggcaaa tccagtgtcg gcgtgatttt tgttttacga tcataaatca
    33481 acacctgatt ttctgtttta tgtccactga aaatctgttt gtcgcgactg cttccttcgt
    33541 aatctgatat tcctttggct tttatgtcat ggaaattgca cccaaacggg acgccggatt
    33601 ttttctcggc tgcgtgttta gcctggttcc accagttgtt tagcgtttta gctatgacct
    33661 tcccgccctt ggttgtgttg atcacatatt cacatgtgcc tgaagataca tttctggcta
    33721 actggatcgc ggttcgtaat cgtggagacc attccttgat ttgtttggtg ccggtcttgt
    33781 tttgctcaat gtagattcct ttatccataa tatcctgcca tttcagctcg agcacatcac
    33841 cgagtcttgc cgcacaaaga taggatatct ccattgcaat gcgtaactgt ggtattgctt
    33901 ccgcatatat cgccgcatac tgttcatctg tgatgtaaac agtgcgggct ttaagcgtaa
    33961 atttcctgac tcctttgcat gggttattct tcacatatcc acgctcatat ccccacccgt
    34021 atacacgact gagacttgcc agttcatggt ttgcctgggt cttgctctca atcccccgtt
    34081 tatccatgaa aattcttacc tgctcaattt ttacattatc agccagtact tttccgaaga
    34141 ctgccagtaa cgccctctga tgttgccgat aatctttttg ggttctgggg gccagttctg
    34201 taaatgcggg ggaatccata aacatgtgcc ataatttagc gacggtcatt atgttgtggc
    34261 gttttgcttt ttccagttca taattctgcc agactttagc tatgctggtt tcccgcactc
    34321 ttcctagccc tatagttttt gtgcttcctt cgggtttcca tacgtaactg taaccattcg
    34381 atctaacacg tggcggtagt gcattatctt ttttgttttt tcttggtctt cccattgttc
    34441 agcgcctcaa aatcggggtc agcagaaacc agttcagata ctttcggtat cgcagtcagt
    34501 ccgcgcggga catcactgcg aagaactatt ggttcgtttt taggaccggg tacaaatggg
    34561 ataccgtgca accttaactg ttgttgctgt tttgtatacc gttcgtatct cgtgatctct
    34621 tgaatttctg ctggcgatag agttaattcg tacatgtggt cacgtgcctt acagcatgac
    34681 cgccgccact atagcttgtg ggcggcgata tgggttgaac attaaaaatc agcctgattc
    34741 gggaacagtt tttgccagat agctgaaacg tattttgcct ggtaacgcgc atcgtgcagg
    34801 gcattatggc gttcaccctc aaatgtgatg acacttcgag catcgaaatc catcaccagc
    34861 cccagggcaa caatcgttct tacatcacga tcattggtgt aacgccacgg gcaggggatc
    34921 tcctgccgtt catatgaacg gcgtaaaatc acgttgtcga agttggctcc atttccccag
    34981 acctgaacaa aaaactcacc ggagttttcg tctataaatt cccgtaattg taaaagcgca
    35041 tcatcaagcg gtattcgatc agctaaaatg gcggcacggg cttcgccaga gcgttgaagc
    35101 caccattcga tggtgcttat gtctacagtg ccgccacatg tacccatatc aattattttg
    35161 ctgaattcag gcccctgctc tccggtttgt ggatcgaaaa atacagcgcc tattgaggct
    35221 attggtgcgt caggattttt gcccattgtt tcaaggtcaa tcatcagatg gtgccacaac
    35281 ctgctggtgg atgtgatttc acgatgaccg ttcaccttaa ttaagggatt tgctgtctcg
    35341 cctgtttcat tattgccatt ggcatgttga tcgccttcgg tgcactcctt gttcgggagt
    35401 ccggcacttt ccatttcctc cggatctttt tcccgggctt catcctggct ttcttcgttg
    35461 aatgtctcct ggtaggttgc gtcccccatc actgcgccac aatcagggca gttaccacca
    35521 ccagtctgat tgcatgcggt acaaactttt tcctcttcct gttgcactac tggttcaggt
    35581 tgtttcgttt ctggctcgtt ttgtaacgca tttgggctgt tttgttccgc tttctgaccg
    35641 ttctgttccg tttcttgctg gttctggttc acagaatcgc aggtttcaat ccccttaacc
    35701 catttcgaat cattcgggtc gctaatccct tcaacaaact caccacgaga agcggcaagc
    35761 aatttgtcgg catctggtcg actgatgtct gctgcctcca taattttatt tacttcttca
    35821 gcagtaactt ttacctgtga cttgtcatcc agcggctgcg ggtcctgatg atgttcaact
    35881 gtatccgctg ccattgtttc agccgttgcc tgttcatctg ccattgcgcc agatggttgc
    35941 gggttttctt catcattttt ttcttcttct gttttgcact tagcagccag tccgccgtta
    36001 atttcttcca ggatatcttt ttccggtgta tgccgggcag ccatgagcgt ttcagctgtg
    36061 ggattctcgt gatcagtttc tgtcaggttg gcattgatat acccctgaag gcgtcccggg
    36121 tagtgataaa actcagggtg ggcgctccgg ataagtgcaa aaatagctgc gcgggaatag
    36181 tccaggatac caggggttgc gcgaagtgct gcggaccatt ccctgaacgg actttctttt
    36241 ttcaggacga tttcttttgc gcgacgataa acgctcacgg gtatttcata aatattaaaa
    36301 tccatcggaa gtgtggctgc tgcagcctcc acatccagag tatcgagggt gtgtactaag
    36361 tcaggattac gatcggtttt atttccgcca ccggcatttg caccggaagc cgtgcgggtg
    36421 atgcgagaaa cacgatttcc tttcatccac tcttttgtca gcagacctcg atcggtatag
    36481 tcagcgtcca ggtatgcttc gaaaaaagca gtcattaacc ccagactgga attaacggga
    36541 ttagggaaaa ctttgtctgt gtcgcgtacc agtttgtgaa ggtcgcgaat ctccagcggg
    36601 tcgagcagct ttgtgttgtg ggaaatagcc agggcagtaa cagccggcag ttcttcagct
    36661 cgtgcgatat gtaatgcctg aagttcttcc cgtgaaacgt gcgttaccgg tttttcgctg
    36721 ccatgttgcg caagccagcg aatgggcagt tcctgaccgg aaatcggcag gagcatatta
    36781 tcctcaatct cagccatgtc ttcgccattg acgttggtat tgtcagtgct ggctggtttg
    36841 tcctgaactg agggggaagg cgctataaat atcattgtga tgccatcttc cccgcctttt
    36901 tcatagcggt tgcagaattc ggtatcaaac acgccttccg gtggaaggtc attcacaacg
    36961 gggaaatgga cgcggacagg ttttttgaaa tcatcagcgt cgtagcctgc ctcgtccatt
    37021 gcaacaacgc accgtgagat ggcaacagat aatttttttg ctgtagtcca gtaaaaacct
    37081 cctttaatcc caaggcgttt tctgaccttg tcattttttg cttcgcaata tagcgaaaat
    37141 tcttctttat cagtgctcat ggataaacct cataactatt ttaaggttgt acgaacccct
    37201 gccattgctg gcatgcttaa tcaacgggta tggcgttaat atggctggtg gattatccag
    37261 ccggtatttc gttattcagg tacagcgata ctttttttaa cgggaggcat tcgccagaaa
    37321 ttttttgctc gtctcttgca cggaggcatg attctttact ggcataaatt ccggtaatca
    37381 cattctgtgg ctcacccgtt ataagaaaaa ccgtcatcac cagtgcaaat gcagaagtca
    37441 ctgctgccct ccgaaaatac caagtttaag aagggcaatt ctggagagta tggaattatc
    37501 attgagaaga taaggctcat attttctcat cttaatggca tcttcagtaa actcccggtt
    37561 actgagcaga ataccaatat caaaacaccc ttcagacgta ttaacgtttg gtaataacgt
    37621 ttccattatc gcgtcctcaa caatgaattt ttagtaattg ctccacagtc atatttttaa
    37681 ttgcgctccg gtttacaaga gtccagcctt gtttctccag ataaaactgg aaggtatcca
    37741 gggtgcagac cattgcacca tcaggaacgg tttcagtgaa tttgatattg ccgtgttcgt
    37801 cgagacggat aaccagggta cgtccgtccc cgtgaatcat tttgtcggga gacggggcgt
    37861 tattctggcg cagttctttc tccatgcgat cgaactcagc gatgtaggct tctttaaatg
    37921 cagcggcttt tttgccggtg aagcccatca ccaggaaaac gaagccgttt ttggtgattt
    37981 ggtacattgg gagtttgcga ccggttgagt cggtatattc gctcgacaca aaattgtgct
    38041 cagtgaattt tgctgaacag tccagattgc gaattttatc caacactcgt tcgtggcgtt
    38101 taccaaagaa ctcagcgatc gcaatagacg tagtgacagc gcgtccattt tcgatggtta
    38161 cgtcagggtg agaaagggca gggatagtag ccatgatggc agcctcgata gtcagtttaa
    38221 ataactcacc accaaggctt tccacgacca taagggtggt gagacgtaca ggggtggaaa
    38281 taccggtgac taccgaaacc ggccagcctt tcggctgccc tgcacgcccc accataatct
    38341 gaatgtggct gtgcattacg cataaaaaaa ccgcctgagc gcggttatgc gcagtagtca
    38401 tcatcgggtt tccacgcccg gcacccgctt tatgaggtgc aggtgcacta taattccacc
    38461 cgttctggtt ttcaatagct acattcaaca ttttctctta cccttcatca ctgaagtgaa
    38521 ctttgttgat gcagtgcctg gtgcctccag gtgacgttaa ccagttaaca attaacgccg
    38581 gaatacagaa ggatgcccgt tacgccccgt aaaagaccac tttacggttt taactgttcc
    38641 gcgtgcgctt agccgcattc accgcatcac aaaattcact ttaaaaaggg cggacatcag
    38701 aaagctaata aactgatgcc gccaaaggta ccactgccat tgaaacatgg agatgttgtg
    38761 gcggggttgt cactcaggca tatggtcaac ctgacaaccc ggtgtcctca atgaggggaa
    38821 aagaaataaa aacccccagc catatttacc gccgcgccat ttcgcggatt gccacaaccg
    38881 gaagcacacg gtcgaagaaa tctaacgaca agccttctaa ggaaaagacg ccttcgccgt
    38941 gtgctttctg ttgtgccctg acttttcagg gtaaattaac cagtggcaac ctgtttttac
    39001 tggtgctaat cagttagtgt ttctggctaa ccagcgacgc gcgccagctt cggttttaaa
    39061 ctttttactt ttggtatacg tcatcgcggt aaacgtgccg tcctggttag ggaacacacc
    39121 gcataccaga gattcgttgt tgccaagatt gagcgtatcc atgttgacct catttcccct
    39181 taacgccggg gtagcggaac aaaaaacctg ctgcatagtt aaacgtaatc cccgccgtca
    39241 tgttcatacg cctcaggctg gctacttaac ccctgaccac tgccgggtaa ctcgaagtat
    39301 tgccctgcgt tctgtggggc ggggtgggtt tatggataca atttacaaat taaaatttaa
    39361 ctggtcaaca tgtttattat taaatttgta attgcgagct gttgcatcag tcccaaaatg
    39421 ggactactat acagttatga agattatctc agttaaaaca ctcagggatt tttgggcgga
    39481 gaatcctgat gcagaacaac cgctaagggc atgggtggat gaggcggcaa aagctgactg
    39541 gaaaagccca gcagacatta aggcacaata ccgaacggcc agtatattga aaaaccggcg
    39601 cgtggtattc aacataaaag gtaatcatta ccgtttaatt gttgcaattg cgtatcagcg
    39661 aggatgggta tttgttaaat tcatcggaaa ccacaaggaa tacgatgcta ttgatgctga
    39721 taccatcgaa ctggagtaag catgaacatc aaacctattc gtacagagca agattatgaa
    39781 gccgcgttgc gtgctgttga acccatgttc gacaatgagc ccgaaattga cacgcctgag
    39841 ggggattttt ttgaggtgat gtgtttgcta atagaggaat atgaaaaaaa acattatccc
    39901 attgagccac catccccaat tgaagctata agatttcgca tggaacagca ggggctgact
    39961 gtgaaagatt tggaacccgc aattgggaaa aaaaatcggg tttatgaggt gttgaatggt
    40021 accagaagcc ttacgttacc aatgattcgc cgtcttcata atcaatttgg tatccccctg
    40081 gaaagcctgg ttggattata aaatctgcta gtcatttgcc tgatgctcgt tccagaaaag
    40141 gaatgcatca ggcagttttg tttttctgcc gcagtaactc ttcaagtttc cgtttataga
    40201 aatcgcgttt ttgctccata tcacgaatga tctgctctgc gtcgctttga ggtaactcat
    40261 ctaaaagcga tatgattttt cgttgttgtt ctgtaagttg cggttggttg tcattactgg
    40321 atacagccat tttatcgccg agagtttctt cttccataaa gaaccaatgg acgggatgtt
    40381 gtgagagctc tgctaatttt tccagtttat ccattcttgg catcacgcct ttcaaccaac
    40441 cttgcacgga ttggggtttt acaccaagac gtcttcccag ctctgactgg tttatattca
    40501 attcctgcaa cacctgctga aggcgtttta caaagatcat caccacccct cgtaaactag
    40561 ttccgcgatc ctacagaaaa aattgataag tggcattaca aatagaagtt gaaatttaaa
    40621 tttaaatttg taatcatcgg tgtcatcgta aagttcggag ggaaacatgc aaaaaagtac
    40681 tcaagtgaaa atcctgtcaa taatgagcca atcagaatta gggcgtcgtc ttggtaaaac
    40741 accgcaaacc ataagtgggt ggtttaaaaa acgagtgcca gcggaggagg ttataccagc
    40801 atgtgaggcg cttgactggg gagtaactcc gcatgaattg cgccctgata aataccctaa
    40861 tccaaccgat ggtttacctg ttgagtatca ggctaacgaa caagcagcgg cgggagttga
    40921 ttcatgaaaa tcaagcatga gcacatccgc atggcgatga atgcgtggct gctttatccg
    40981 agggtagggc gcaaaaaaat cgctgatgat atagcgacag catattttga gcttgaaatg
    41041 acttatccac caatgcatga cacctctacg acagagggta ttggattgaa tatacaaaat
    41101 atttttcgct ggcttgaaaa ggatacgcct gatgctgttg aaaaaattca ggcactaatt
    41161 ccggctatcc tgactgttct tccgcgtgaa ctgcgttatc acctcagtat ttttgacact
    41221 gttgagcgcc gtgcattact ggcggcgcag gaagcgttga gcacggcaat tgatgcacat
    41281 gatgatgctg tccaggccgt ttaccggaaa gcgtatttca gcgacggcgg gtcatccggc
    41341 gagtctgttg tggtgcattg atatttatgc cagccccctg ctgattctgt tgattgggga
    41401 atcacagaat atcaccagag gatggttcgt cacaagatga ggcaattatg gccgcattac
    41461 catacatgca actgtacata gctgattacc tagctgacac catgcatttg tccgcagagg
    41521 agcacggtgc gtatttgttg ctgatgttca attactggca aacagggaaa ccaataccta
    41581 aaaacaggct ggcaaaaatt tcccgtctga ctaacgagcg atgggctgat gtggaaccat
    41641 ccttgcggga gtttttttgc gataacggcg atgaatgggt gcatcttcgg attgaggaag
    41701 atctggcatc agtcagggaa aaattaacca aaaaatcagc cgccggaaaa gcatctgttc
    41761 aggccagaag aagcagaaag gaagcatatg ttcaaacaaa acaagagaga gatttaacag
    41821 gcgttcaaac aaatgttggt gttgtgtttg aacatgacgc aaacacaaaa gcaactaata
    41881 aagatacaga tctaaaagaa ttaaacccca cacataacgt gcgtgaacgc gagagtattc
    41941 cgaccagtga gtcgcatggt gcgccgttgc agacagccga acctgaatac ctggacggcc
    42001 tgagcgaacc gatcgggaaa ttttcgatga ccactgtctg gcagccgtcg ccggattttc
    42061 gacaacgggc agcagtgtgg ggtatggctc tgcctgagcc ggaatttaca cctgctgagc
    42121 ttgccgcatt ccgggattac tggatggcgg agaggaaggt tttcacacag gttcagtggg
    42181 agcagaaatt tgcccgccac gtgcagcacg tcagggcaca ggtaaaacca gtcagcaagg
    42241 ggggaagcca tgcagcatca ggtggcacgg catcacgggc agttcaggaa atccgggcag
    42301 cacgcgaaca gtgggaacga gacaacggat ttatcagcaa cggaaacggc ctggaagctg
    42361 tgggagctta tgggggaggt gtattcgaac cgctggactc agaagaacgg ggccgcacct
    42421 tcgaagctct ggattgccca gattggtgcg atgactgaac agcaaatccg gcaggtctgc
    42481 cgtcagtgca tggaccgctg ccgggcgggt gaaacgtggc ccccggacct ggctgagttt
    42541 gttgcactga tttcggagag tggggcaaat ccatttggtc ttacggtgga tgcagtgatg
    42601 gaagagtacc ggcgctggcg caatgaatcc tggcgatacg acgggagtga taaatacccg
    42661 tggccacagc ctgtgctgta ccacatctgc ctcgaaatgc gtaccagagg gattgagcgc
    42721 cagatgacgc agggtgagtt aaaacgactt gcggaacggc aactgacgaa atgggcaaag
    42781 catgttggta acgggatgag tgttccgcca gtgcgacgac aactggaagg ggcgaaatac
    42841 ccgaaagggc caacgccaat tgaatggctg aaacaggaat acgaacgccg gaaggcagct
    42901 ggttttattt gaatctgaga agcgattttg taggaggaaa ttttaatgga aaccgttttt
    42961 gacgcactga aagcaatggg aaaagccagc tctgttggac tggctgagcg gcttgatatc
    43021 agtcgtgaag aggttctcaa cgaactgtgg gaactcaaaa gaaatggcgt tgttgataaa
    43081 gcgggtcaca tctggtttct ggctggcgaa ggtgaatccg gggtaaccga agagcagcca
    43141 gaacagtctg aagtaccgga tgtgctgacc ggggaggtcg aacaaaaagt taccgcagac
    43201 atgatgattg agtttatcgg tcaggagggg gctaaaacgt gtgaggaact ggcggataag
    43261 ttcggtgtta gcattcgcaa ggttgcttcc acgttggcgg tggtaaccgc aacgggacgc
    43321 ctggcacgcg taaatcagaa cggtaaattt cgttactgca tgccgggcga taatttacca
    43381 gcagagccga aagccgtgcc ggtaacggaa aatgatggta aggcctttcc tcagccagca
    43441 ggtgttgcgt taccagtaca ggaatctgca acacaggaag atattaaaac agaaacagtg
    43501 gcggacattg tgcagtcgtt gccatcgttt accgaaacgc aagcagatga gctgattttt
    43561 ctgtccctgt gcagggcaaa cctggcgctg cgcagggcga aaagtgatgt tcagaagtgg
    43621 gagcgagtct gtgccgcgct gcgggaactg aacaaacaca gggatattct ccgggatatt
    43681 accgccacca gagagctgct gcggtgagtg gctggaagaa gtggcgctgg gcggaaatta
    43741 tgatactccg gcagtgtgcg ggaacgatga cagtcgaaag catcggcagt ctgattggcc
    43801 gtagtggggc tgccgtcagg acgaaagcgc gggaactggg tatctgcatg atgttacggg
    43861 gtgattatca ccagtcagcc aaatgttcac agcgtgatat tgagctggcg cggcaactgc
    43921 atcagcgtgg cgtaccccga cgggaaattg ccgaaaagtt tgggatgaag ttgggcgcag
    43981 tgaataacta cgtttatttc gacaggaggg ttcaggcgtg agggtgagaa tttatatcgc
    44041 cggtccgatg acgggatatg aaaatttcaa ccgtgaggcg tttcacaggg cggaagaagc
    44101 actgaaacgg gaagggcata ccgttttaaa cccggcagta cttccggacg gactgacaca
    44161 gccacactac atggatattt gcatggcgat gatacgctgc gtggatgcga tttacatgct
    44221 gaaaggctgg cagcggtcgg caggcgctaa ggcagaactg gcactggcgg agaaactggg
    44281 acatgcggtt attttccagg aggcaaccag tgagaaaaat taattatcag acgctacgtg
    44341 agatagcaaa actggcaacc cagggcgaat gggtcgcatt catttcgccg ggcactgata
    44401 catatgcggt gcatacgccg ggggatgaac gttgcggaga cattatcaaa tggcctggct
    44461 ttgatgacca gaaaaatgca gagaacaacg cagaatttat tgcagctttc aaccctgaag
    44521 tagtacaggc actgctggat gaacgggaag cccaaagcaa acgtattgca gagctggaag
    44581 ctaatcttgt ggcgctggcg gcggagaatg cacttgctcg taaagcagtt caggcattct
    44641 gcgatgttgt tggcgacaac accgaggtta tctgcgagga ggttgggaga gatggcgttc
    44701 tggttatttt ggaggccatg aaggcaacag gaaatatgcc agccaccgat gctttcctgt
    44761 ctgaagtgcg ggcgctgggg gtggatgctg ctatagaagc tgcaaaaaat ctggtggccc
    44821 aagaatatga gtataaggat ttcaaagcgg cgcagagtga ttgctgtatg caccctggtt
    44881 cagacctgat agggaaggtt gaaatgactg agtggttagt tgactttgct gcccagcttc
    44941 gcaaaggagt tcagtcatga gagcttcaat tagttatgtc gacgattgcc acctaagcgt
    45001 gcgtgttgat gaaattgtgt catccgtgcc aacttttccg acaaaaaatg cagccgttaa
    45061 tgcaggcgcg ccattcggat ggaggacggc agttcgtatt gagcgtcgat tcgaaaacgt
    45121 ttgggtggta ggtaagaaat gcttccaaag cgatcgctct gcggggctta attttgaagc
    45181 atatcgattc ccgttcctcc gatgggagaa agagggcggg attacaaaat gcccgattct
    45241 ttccgtgcgc agattcaagc aggaggcaac cagtgagcaa gattgactat caggcattgc
    45301 gtgaggcagc acaaaactat cggtcgatgc tggcgtggta tcaggagaaa ccagacagtc
    45361 caaacgctga gcaggattgt gatgcagctt tggctgcgtt taagcgtgaa atccgtcatc
    45421 gtgaagtggg tattatcgct gatttgctgg atgaactgga ggaagcaaaa cagcgtatcg
    45481 acgagcagga gtcccgcata gtgaagttac cagaaccatt caagctggct aaatcatcaa
    45541 gtggattaac gtactactac gctgatgagg tcaatgccgc actgactgct gctggtattc
    45601 gcatagaagg agagtgagat gaccactatt accaaagagc gactgcaatg gctggctaac
    45661 atttctggcc gcgatgacat tgacgatata gacggcggtg aaattcgtga gctggcgctt
    45721 atcgcgctgg catcgctgga agcgaagcct gtagcttcat gtattattga agatgggggc
    45781 atgtgtgttg acgggttcgg tgagtatgtt ggtcactcgc tgcctgatgg aacgcaccag
    45841 ctttacgccg ccccgccagt gcctagtaag ttgccgcgtg aatacagaaa cggttggcct
    45901 cttgcatata gtgattatgc tgaaggctgg aacgactgcc gcgaagccat gcttcaggga
    45961 gataaatcat gattaatcgt atcaagctgg agcacatcct cgaatatgcc aggcagcaga
    46021 agcatactgg tcagcattgt aaaattccac caggggatat ggttgaaatc atggagattg
    46081 ccatgcgcaa ggctggcaat tctccggtaa ctccggatgg ttggataagc tgtagtgagc
    46141 gaatgccgcc agttggtgtt gatgtactac tgtttgtgtc cagcacgggt gaacagttta
    46201 caggttttaa cctggataat acgggtgact tccagtatgc gcagtgtttc ggtgcgccta
    46261 tcgtgtgtga agccacacac tggatgccgc taccagaacc gccgaaggag gtgcgccaat
    46321 gagctggcct gaggccttct caaatgttgg tattgcaatg gcggtggcgc tggtggggta
    46381 ttcgatttgc cgctggggat aaatcgccga aaaaagatcc cgccacaaac atgagccggg
    46441 atctttgatt tatatagcct acgaatccgc agtaagagag gggcagacgg tttattctaa
    46501 caccggaatg atgtgggtaa aagtttataa gaaatcggtt tcataacttt gcccaccatg
    46561 atagataccg acaataaaga cttttctgcc atcaacggca aaagcaataa tcgttctgtg
    46621 gcggaaatga gttacccgca tcccctggcg aatatcatcg cgtttattgc cccgatgcgg
    46681 gaatgtagaa aacccatcaa gataatcaag aagcgcattg gcataattgt cagcaatgat
    46741 gttccctgct ttctccgtta tatatctgtg caggttgatt atttgttgtt cggcctcagg
    46801 agtaatgatg acttcatatg tcatgcagat tacttcccgg atcgaatcgc gacgcgaacc
    46861 tgtgaaatgg agcgtccgtt gtttgggttt tcgcggatag aatcaagaga gggggcggct
    46921 gaatgcgtta accacgcttc gattgcttta tcgcgctcat tcagtgcgcg aagcccttca
    46981 cgaatgacct cgctttctga agcataggcg ccggaagcca cacgggcgcg caccatgtca
    47041 gccatctcgt tagttaatgt aatgctgaat tgttgggttg tacgcatggt aaacctcacg
    47101 gagtaggata gaacactatt cgatgatagc acgctgcctg ttgacgacaa cagaaatcag
    47161 agacaatatt gccgcacgcc agcctgaaca actggcacct gctgcgccag cagagacaac
    47221 cgatggcgca agataccaaa ttacacaatt cggataactc caccgcccct gccagcaggc
    47281 acgggcggcg ttctcatgca ttcaaatctg actggttcca gcatgaccca tgcactgaag
    47341 aacaggccga atggctgatc cagtgctacc gcagacgtgg ttacgagttt cagaaagatc
    47401 tcagcttcga tcgtcgtcac tggataatct ccgtcaggct cccttattcc gaacgcccac
    47461 cgcgtccgtc ccgcacattc cagcagcgca tctggaggta acgtgcgggt attacttcga
    47521 cctgttctgg taccggaact cgggatggtg ctccttaagc cgggccgtga atcaatgtca
    47581 gcattccata acggcagaat attggtggaa ccggaaccga aaaacatgcg cggtctgccg
    47641 tccggagtcg ttcctgccgt tcgccagccg ctggcagagg ataaaacatt actgccattt
    47701 ttcagcgatg agcgggttat tcgtgcagca ggtggtgcag gtgcactgtc tgactggtta
    47761 ttacgtcacg tgaaatcctg ccagtggcca cacggtgatt atcatcacag cgaaaccgtc
    47821 attcaccgtt acggtaccgg cgcgatggtg ttgtgctggc actgtgacaa ccagctgcgc
    47881 gaccagacat cagaatcact cgatcaactt gctcagcaga atctggttgc ctggatgatt
    47941 gacgtcatcc gtcacgcaat aagcagtacg caggagaggg agttatcgct ggccgaatta
    48001 tcctggtggg cggtctgcaa tcaggtggtg gatgcactac ctgaggcagt agtgcgtcgt
    48061 tcgctgggat taccagcgga aaaaatccgc tccgtatacc gtgagagcga catcataccg
    48121 ggagagcagg ccgccaccag catactgaag cagcgcacaa aaaatattgc gctgccactt
    48181 cacgtccacc agcaacaaaa tcccccccag gaaaagacgt tagtaagcat cgccgttgat
    48241 ccggagtctc cggctcagta cctccagcgc cagaaaccac aacgggaaga gatgcctgta
    48301 tacacgcgct gggtaaaaac gcagaaatgc atgacgtgtg gcaatcaggc agatgatccg
    48361 catcacatca ttggtcatgg actgggaggt atgggaacaa aggctgacga tttgtttgtt
    48421 attccgttat gccgtaaatg ccataacgaa ctacacgccg gggtaaaaga ttttgaagag
    48481 aaacacggca gtcagctgtt gttgctgatt cgttttttaa tgcacgcgag aaattcgggt
    48541 gttctgaagt ggaaagcata aatgaccgag cgcatagaat ttgttttgcc ttacccgccg
    48601 acggtgaaca cttactggcg acgccgtggc agcacatatt ttatatcgga ggagggaaag
    48661 cgctatcgcc gggcagtggc gcttattgtt cgccagcagc ggctgaaatt aagcctgtcc
    48721 ggacggctgg caataaaaat tattgcagag ccaccggata agcgtcgtcg tgacctggac
    48781 aatattctga aagcaccgct ggatgcgctg acgcatgcgg gagtgcttat tgatgacgag
    48841 cagtttgatg aaatcaatat tgtacgtggc aagccagtat ctggtggacg gctgggggtg
    48901 aagatttacc ccataatgct tgaagggcag gtcaaaaaat gaaactggaa gatttaccga
    48961 aatactactc cccaaaatcc cccggcctga ctgatgcatc ggcctcaacg tcgaaagatg
    49021 cgctgagtat cactgatgtg atggccgcgc agggcatgac acagaatcgg gctgagatgg
    49081 ggttttctgc gttccttggg aaaatgggca ttagtatgaa tgacagagaa cgggcaacag
    49141 aattgctgac agaatatgca ctcagtcggt gtgattgcgt ggcggcgtta agaaaactcc
    49201 cggcagaaat aaaaccggca gtgatgcgta ttatggcttc gtatgcgttt gaagattatg
    49261 cccgtagcgc ggcgagcaaa aaacagtgct cctgttgtca tggaaaaaaa tttattgaaa
    49321 gcgaggtttt tacaaacaag atccagtatc cggatggtaa gccgccagtg tgggcaaagt
    49381 gcacaaaagg cgtgtatccg tcttactggg aggaatggaa aaaagtcagg gaggtggtaa
    49441 aagttgcctg tccggagtgt ggcggaaagg gggaggtttc caccgcctgt aaagattgtc
    49501 gtgggcgcgg tgttgccatt catcgtgaag agtctgaaaa acggggtatg cctgttatca
    49561 gggactgcca gcgttgtggt ggtcgtggct atgaaagatt gccgtcaaca gaggcattta
    49621 atgctatatg cgaggtgaca aaccagataa cacgcgcgtc atgggaaaaa acagttaaga
    49681 aattctatga tgcgctggtg agcaagtttg atattgagga agcatgggct gagaggcagt
    49741 taaaaaaggt aaccaggtaa caaggttgat ttttccggaa tctgtggtaa atttgcccta
    49801 acgatgggcg ttttatgcct gacgttagaa gactttttac acccgtcgcc aggcgggttt
    49861 ttttatgact gaaatcactc cagtacagta aacgcgctgg cggcggtgaa taccggtctt
    49921 tcagcttgct ggctttttcg acaagagtta ttggtgtgtc acgttaaccg gaaaagggaa
    49981 aaagacatgc tgaaacagca ggatatgacc gaaaccgcca gagtggtgtt taatgaatta
    50041 agcgtcaccg aaccggcgac agtcggggag attgcgcaga atacgtacct ttcacgcgaa
    50101 cgctgtcagt taatactgac ccagcttgtt atggcgggtc tggcagacta tcagttcggt
    50161 tgttacagac gccttcagtc ctgaaggctt ttttatttgt ggtaaatggg cggctggtgg
    50221 gtgttagggg cactcaccag ccatctgctc atgcgtccgg atcacaagca aacctcaggc
    50281 ccactgcttt gcgcaaaagc agaatgagcc tatcagagaa gtgcttattg atctatggct
    50341 aatactgtaa aaatatccag ttgtgagcta atcaacgctg attgcctgga atttatccag
    50401 accttaccgg aaaactctgt cgatctgata gtcacagacc cgccatactt taaagtgaag
    50461 c
//
LOCUS       DAZFKF010000027        50402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFKF010000027 DAZFKF010000000
VERSION     DAZFKF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.027"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(188..589)
                     /gene="nikR"
                     /locus_tag="ACHKA1_001938"
     CDS             complement(188..589)
                     /gene="nikR"
                     /locus_tag="ACHKA1_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO5679594.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(595..1401)
                     /gene="nikE"
                     /locus_tag="ACHKA1_001939"
     CDS             complement(595..1401)
                     /gene="nikE"
                     /locus_tag="ACHKA1_001939"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO5679595.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLERASEMLKAVDLDDSVLNKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            complement(1398..2162)
                     /gene="nikD"
                     /locus_tag="ACHKA1_001940"
     CDS             complement(1398..2162)
                     /gene="nikD"
                     /locus_tag="ACHKA1_001940"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO5679596.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAAALGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLRTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2162..2995)
                     /gene="nikC"
                     /locus_tag="ACHKA1_001941"
     CDS             complement(2162..2995)
                     /gene="nikC"
                     /locus_tag="ACHKA1_001941"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO5679597.1"
                     /translation="MNFFISSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPNAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(2992..3936)
                     /gene="nikB"
                     /locus_tag="ACHKA1_001942"
     CDS             complement(2992..3936)
                     /gene="nikB"
                     /locus_tag="ACHKA1_001942"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO5679598.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(3936..5510)
                     /gene="nikA"
                     /locus_tag="ACHKA1_001943"
     CDS             complement(3936..5510)
                     /gene="nikA"
                     /locus_tag="ACHKA1_001943"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5679599.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5621..6208)
                     /gene="acpT"
                     /locus_tag="ACHKA1_001944"
     CDS             complement(5621..6208)
                     /gene="acpT"
                     /locus_tag="ACHKA1_001944"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO5679600.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6210..7439)
                     /locus_tag="ACHKA1_001945"
     CDS             complement(6210..7439)
                     /locus_tag="ACHKA1_001945"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFO5679601.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(7436..8167)
                     /locus_tag="ACHKA1_001946"
     CDS             complement(7436..8167)
                     /locus_tag="ACHKA1_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFO5679602.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(8167..8631)
                     /locus_tag="ACHKA1_001947"
     CDS             complement(8167..8631)
                     /locus_tag="ACHKA1_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFO5679603.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCAAGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(8628..9797)
                     /locus_tag="ACHKA1_001948"
     CDS             complement(8628..9797)
                     /locus_tag="ACHKA1_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFO5679604.1"
                     /translation="MIYISAVGMINALGNNLDEIATNLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPVIPEKFAAHRSRNNQILLAALAQIQPQIDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSPTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQLIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(9799..10383)
                     /locus_tag="ACHKA1_001949"
     CDS             complement(9799..10383)
                     /locus_tag="ACHKA1_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFO5679605.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRQGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(10380..12698)
                     /locus_tag="ACHKA1_001950"
     CDS             complement(10380..12698)
                     /locus_tag="ACHKA1_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFO5679606.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAAALLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDVKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSRGLPVVLALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKFTTSLENA
                     KKEGLISNYRTIPLNSLARQEEDLHLLTTAAPTVTKALQNAGLTTVNPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKNSAALQEISSYYPCGIAWVDRKNTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAITLAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(12667..13272)
                     /locus_tag="ACHKA1_001951"
     CDS             complement(12667..13272)
                     /locus_tag="ACHKA1_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFO5679607.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGK
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            complement(13269..13691)
                     /locus_tag="ACHKA1_001952"
     CDS             complement(13269..13691)
                     /locus_tag="ACHKA1_001952"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFO5679608.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            complement(13695..15371)
                     /locus_tag="ACHKA1_001953"
     CDS             complement(13695..15371)
                     /locus_tag="ACHKA1_001953"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO5679609.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDSLLAEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKHATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTASRARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCETFADP
                     LLLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(15362..15715)
                     /locus_tag="ACHKA1_001954"
     CDS             complement(15362..15715)
                     /locus_tag="ACHKA1_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFO5679610.1"
                     /translation="MKPHEIERHQAQTNHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALNLLAPGWRFHSIQNIKFQSPLLPENRVTLALNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(15702..17063)
                     /locus_tag="ACHKA1_001955"
     CDS             complement(15702..17063)
                     /locus_tag="ACHKA1_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFO5679611.1"
                     /translation="MKQTLPLARWLTAPRPDDTPIAWLNESTWTLGDLRHDVAQLICR
                     LQQQPGERWALCFENSYLFIVALLATLHAGKTPVLPGHNRVIQLNEQRELFDGVLSDS
                     ELNWQGSLLLVASSPQIATQSFTFAAIAPEAFIELFTSGSTGQPKRVIKPVHLLDREA
                     ELLAERLGARLAGCRVVGSVLPQHLYGLTFRVFLPMALGLPLHAAMLWYVEQFAALSH
                     QHRYIFISSPAFLKRLDTQLSPPPVQVLLSAGGELPWQDVQHTASWLRVWPDEIYGST
                     ETGVIAWRYREQEQRRWLPLPGMQFQAEGDAFRLFSPLIAEDNGLLLDDILQFSEDGQ
                     FHLMGRRGRIVKIEEKRISLQEVEQRLLALDGIREAAAVPVTRGGRQCIGVLLVLDDE
                     AHLQWQNGGGHSQEQAWRRLLHPMLEPVAIPRYWRVIDEMPVNSMNKRVYAQLQELFH
                     EAP"
     gene            complement(17060..17641)
                     /locus_tag="ACHKA1_001956"
     CDS             complement(17060..17641)
                     /locus_tag="ACHKA1_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679612.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQPRRQAGPLQAVTQIVAVVGIALCVASFMLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLVRRQVMKRDRA"
     gene            complement(17646..17897)
                     /locus_tag="ACHKA1_001957"
     CDS             complement(17646..17897)
                     /locus_tag="ACHKA1_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5679613.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPKDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            complement(17909..18166)
                     /locus_tag="ACHKA1_001958"
     CDS             complement(17909..18166)
                     /locus_tag="ACHKA1_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFO5679614.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(18141..18962)
                     /locus_tag="ACHKA1_001959"
     CDS             complement(18141..18962)
                     /locus_tag="ACHKA1_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO5679615.1"
                     /translation="MSKLMSRLEWTWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPDRPFF
                     TVAVHGRVEINKFYDATSQEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            complement(18959..19681)
                     /locus_tag="ACHKA1_001960"
     CDS             complement(18959..19681)
                     /locus_tag="ACHKA1_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFO5679616.1"
                     /translation="MKFTLNITDWQALAPGLSEAQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPIVSSSLSAGRDTFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALVIEAGDDWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNVDTFSLPGERVQWRWSHR"
     gene            complement(19722..20780)
                     /locus_tag="ACHKA1_001961"
     CDS             complement(19722..20780)
                     /locus_tag="ACHKA1_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO5679617.1"
                     /translation="MYEQDSLSALDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQ
                     GKHGATLADITEHSHINEYAASVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(20849..21226)
                     /locus_tag="ACHKA1_001962"
     CDS             complement(20849..21226)
                     /locus_tag="ACHKA1_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5679618.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIDQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQGIIKARQQSRDHSAEIRINYTASSYDINYENSQNLQASGGQIHK
                     NYNRWVRNLDKDIQLNLSAGADL"
     gene            complement(21618..22667)
                     /locus_tag="ACHKA1_001963"
     CDS             complement(21618..22667)
                     /locus_tag="ACHKA1_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO5679619.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVKRPVAITIVVVVMLITLTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            22799..24016
                     /locus_tag="ACHKA1_001964"
     CDS             22799..24016
                     /locus_tag="ACHKA1_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5679620.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMAVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(24020..24577)
                     /gene="dcrB"
                     /locus_tag="ACHKA1_001965"
     CDS             complement(24020..24577)
                     /gene="dcrB"
                     /locus_tag="ACHKA1_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO5679621.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(24650..25315)
                     /locus_tag="ACHKA1_001966"
     CDS             complement(24650..25315)
                     /locus_tag="ACHKA1_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO5679622.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLTDKSEINAL
                     QAS"
     gene            25536..25781
                     /gene="tusA"
                     /locus_tag="ACHKA1_001967"
     CDS             25536..25781
                     /gene="tusA"
                     /locus_tag="ACHKA1_001967"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO5679623.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(25883..28081)
                     /gene="zntA"
                     /locus_tag="ACHKA1_001968"
     CDS             complement(25883..28081)
                     /gene="zntA"
                     /locus_tag="ACHKA1_001968"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO5679624.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(28155..28781)
                     /locus_tag="ACHKA1_001969"
     CDS             complement(28155..28781)
                     /locus_tag="ACHKA1_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO5679625.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISSFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            28922..29281
                     /locus_tag="ACHKA1_001970"
     CDS             28922..29281
                     /locus_tag="ACHKA1_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO5679626.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(29284..29553)
                     /locus_tag="ACHKA1_001971"
     CDS             complement(29284..29553)
                     /locus_tag="ACHKA1_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO5679627.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(29543..30139)
                     /gene="rsmD"
                     /locus_tag="ACHKA1_001972"
     CDS             complement(29543..30139)
                     /gene="rsmD"
                     /locus_tag="ACHKA1_001972"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO5679628.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAKCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLYREKVAGQVAYRLYQREAQGESDAD"
     gene            30289..31785
                     /gene="ftsY"
                     /locus_tag="ACHKA1_001973"
     CDS             30289..31785
                     /gene="ftsY"
                     /locus_tag="ACHKA1_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO5679629.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKDEM
                     GEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDAEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            31788..32456
                     /gene="ftsE"
                     /locus_tag="ACHKA1_001974"
     CDS             31788..32456
                     /gene="ftsE"
                     /locus_tag="ACHKA1_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO5679630.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            32449..33507
                     /gene="ftsX"
                     /locus_tag="ACHKA1_001975"
     CDS             32449..33507
                     /gene="ftsX"
                     /locus_tag="ACHKA1_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO5679631.1"
                     /translation="MNKRDAINHIRRFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            33752..34606
                     /gene="rpoH"
                     /locus_tag="ACHKA1_001976"
     CDS             33752..34606
                     /gene="rpoH"
                     /locus_tag="ACHKA1_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO5679632.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            34877..35980
                     /gene="livJ"
                     /locus_tag="ACHKA1_001977"
     CDS             34877..35980
                     /gene="livJ"
                     /locus_tag="ACHKA1_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO5679633.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(36180..36563)
                     /gene="panM"
                     /locus_tag="ACHKA1_001978"
     CDS             complement(36180..36563)
                     /gene="panM"
                     /locus_tag="ACHKA1_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO5679634.1"
                     /translation="MKLTIIRLEKFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            36987..38096
                     /gene="livK"
                     /locus_tag="ACHKA1_001979"
     CDS             36987..38096
                     /gene="livK"
                     /locus_tag="ACHKA1_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO5679635.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            38144..39070
                     /gene="livH"
                     /locus_tag="ACHKA1_001980"
     CDS             38144..39070
                     /gene="livH"
                     /locus_tag="ACHKA1_001980"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO5679636.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            39067..40344
                     /gene="livM"
                     /locus_tag="ACHKA1_001981"
     CDS             39067..40344
                     /gene="livM"
                     /locus_tag="ACHKA1_001981"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO5679637.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            40341..41108
                     /gene="livG"
                     /locus_tag="ACHKA1_001982"
     CDS             40341..41108
                     /gene="livG"
                     /locus_tag="ACHKA1_001982"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO5679638.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            41110..41823
                     /gene="livF"
                     /locus_tag="ACHKA1_001983"
     CDS             41110..41823
                     /gene="livF"
                     /locus_tag="ACHKA1_001983"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO5679639.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            41942..42688
                     /locus_tag="ACHKA1_001984"
     CDS             41942..42688
                     /locus_tag="ACHKA1_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO5679640.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLSETGADRHGHTLLMRLQNKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            43048..44364
                     /gene="ugpB"
                     /locus_tag="ACHKA1_001985"
     CDS             43048..44364
                     /gene="ugpB"
                     /locus_tag="ACHKA1_001985"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO5679641.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            44462..45349
                     /gene="ugpA"
                     /locus_tag="ACHKA1_001986"
     CDS             44462..45349
                     /gene="ugpA"
                     /locus_tag="ACHKA1_001986"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO5679642.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            45346..46191
                     /gene="ugpE"
                     /locus_tag="ACHKA1_001987"
     CDS             45346..46191
                     /gene="ugpE"
                     /locus_tag="ACHKA1_001987"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO5679643.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            46193..47263
                     /gene="ugpC"
                     /locus_tag="ACHKA1_001988"
     CDS             46193..47263
                     /gene="ugpC"
                     /locus_tag="ACHKA1_001988"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO5679644.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            47260..48003
                     /gene="ugpQ"
                     /locus_tag="ACHKA1_001989"
     CDS             47260..48003
                     /gene="ugpQ"
                     /locus_tag="ACHKA1_001989"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO5679645.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNYKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(47990..48430)
                     /locus_tag="ACHKA1_001990"
     CDS             complement(47990..48430)
                     /locus_tag="ACHKA1_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO5679646.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            48550..50295
                     /gene="ggt"
                     /locus_tag="ACHKA1_001991"
     CDS             48550..50295
                     /gene="ggt"
                     /locus_tag="ACHKA1_001991"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO5679647.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
BASE COUNT        11630 a        13293 c        14045 g        11434 t
ORIGIN      
        1 tattatttta atgacagtat cttcatacga aaaagcgaag aaaaacttgt ggtcacaatg
       61 aattgaagat ttttaggaat atatttagaa cgttacaaaa gagaaggttc ggatagggta
      121 tttagagagg aatcaggtgt gctagtggcc ctttgttgat cgttcaccaa aggacccaat
      181 aatagactca atcttccttc ggcaagcact gcaaatgccc gtgccgcacg ccgcgctggg
      241 cgataacgtc atcggcaaaa tgctgcacgt cacccatgtc acctttcaac acggcgattt
      301 ccagacagtc gtcgtggttg atgtgcacat gtagcgtggc gacggagagg tcgtggtgat
      361 gatgctgggt ggaaacaata cggctggcta agtcgcgttt ttcgtgttca tacacatacg
      421 acagcaccgc gaaaccttgt gtgccgtgct gctgggtagc ttcctgcgcc agggcgctac
      481 gcagaatatc gcggatagct tcggaacggt tgttataacc acgacgctgg ctcaggctgt
      541 ccagcgtctc cagtaaatcg tcatcaagcg tgatggtgac tcgttgcatt tgcgttaaac
      601 cttttctgag gtgcgacggc gcacggggaa tgcgggtaat accgcgtttt gtagcacacg
      661 tccggcgtca gaggaaaagg ttaatttatc tcccaccgcc tgggtttcga cgatttgtcc
      721 gttgtccatc accatcactc gctggcaaaa gcgttccacc aggcgtaagt cgtgggtgat
      781 gaacaggcag gcggtgccaa actgttgttg tagctttttc agcaggcgaa tgacgcccgc
      841 ctgtaacacg agatcaaggt tagaaactgc ttcatccaga atcagcagtt tcggttcgac
      901 cgccagcgcg cgagccaggc agacgcgctg gagctggccg ccgcttaact gcggtgggcg
      961 cttgttgagc acgctgtcgt cgagatcgac cgccttcagc atttcgctgg cgcgctccag
     1021 ttgttcggat tttttcagtg atagcaggtg acgcatcggt tctcgcagga tctcgcgcac
     1081 ggttttgcgc ggattcacgg cgctgatgga gtcctgaaat accatctgaa tatcgcggcg
     1141 gaacgcttta cgctgggcgc gattgagctt tgccaacgat tcgccgcgcc agctaatatt
     1201 cccttgtgag ggagactcta agcctaccag cagccgcgcg agggtgcttt tcccgcagcc
     1261 gctgcgcccc agcagggcga cggtttcgcc gcttttcagg gtcagggaaa cgttattcag
     1321 caccgcctgg tgctgatgtt ttccgctaaa tccaccgtgc gcatagtgat gggaaaggcc
     1381 ggaaacgtta agtaaagtca tgatgccagc tccataccgt agagggcgag atgagcggaa
     1441 accaggctgc gcgtcaccgt atgtttgggg gcgttaaaca gcgtttcgac atcgccctgt
     1501 tcgacaatct taccgtgcga catcaccgcc acgtcatccg ccagacgcgc cactacgccc
     1561 atatcatggg tgaccagcag catccccggt gcctgttttt gcataatgct ttccagcaga
     1621 tcgaggatgc gcgcctgtgc taccacgtcg aggtcggtgg ttggttcatc ggcgatgata
     1681 aacggtgatt cgcaaagcac cgccatcgca atcatcatgc gctgcaacat gccgccgctc
     1741 atctcgaacg ggtacagctt cagcacgcgt gcggcgtttt ccagccccac cgcttctatg
     1801 gcagcggtaa gcgtagcgtc atcggcgggt ttccccagcg ccaggcaggt ttcacgcgcg
     1861 tgggtgtgca tggtgcgtag cggattaaag gcgctgcgcg ggttctgcat gatggtggca
     1921 attttgatgc cgcgcagggc gcagggagaa accggtttgc catcggctaa aatttccccc
     1981 gccgtctggc gaacgcctgc gggcagaatt cccagcgccg cggcgcaggt taatgatttc
     2041 ccgctgccgc taccgccgac taacgccagc acacgcccgc gtttcagggt taacgatacg
     2101 ccgtgcacca gcggctgcgc ggcctgtagc gcgatattac gtagttcaat ctgttgcggc
     2161 attagtgtgc gtgctccgtc accagatgag ggtccagatg atcgcgcagt gcgtcaccca
     2221 ccaggttaaa ggccatcacg ctgataaaca gcgccagccc cggccagaac atttgcagcg
     2281 gctgggtcca gatatactgg cgcgcgtcgt taatcatcac gccccattcg gcggtcggcg
     2341 cggtcacgcc gaggccgagg aaggacatcc ccgcgacgtg cagcatcata tggccgatat
     2401 ccagcgttgc cagcaccagc agcgaaggga tcaccgcacc tgccagatga tcaacaaaca
     2461 ctcgcacatg gcccgcgcca gaaagccgtg atgccagcac aaactcgcgc tggcgcagtg
     2521 aaatcaccag gctgcgcacc atgcgtgcat accacgccca gtgcgacagg gcgatggcga
     2581 taattacgtt ggtcagccca gtgccgagca cgccaaccat aaagaacgac agaatcgagg
     2641 tcgggaaggt cataaacata tcggcgacgc gcatggtagc ctgatcaacg cgcccgccaa
     2701 tcagcccggc gctgccgcca ataaccagcc ctaatgtcag caccagcagc aggcaggcca
     2761 ttaccgaacc gagcgacacg cgggtcgctg ccatcagccg cgagaaaata tcgcgaccta
     2821 agtgatcggt gcccagccag tgctgcgcat tcggcgaaag caggcgcgac ggcaaatcaa
     2881 tcgcctgggg gtcatacggc agccaccact ggctggtgag cgcaatcagc gccagcaggg
     2941 cgataatgat cagcgccagg cgtaccgacc agcgggaaga gatgaaaaag ttcacgcgtg
     3001 cgctccttca tgacgacgaa tgcgcgggtc cagcgcggcg ttgagcaaat cgacaatcaa
     3061 attacagacc acaaaaacca ccaccatcat cagcgtaaag cactggatca ccggatagtc
     3121 acggttaaaa atcgccgaca ccgcatagcg cccgacgcca ggccaggcaa agatgttttc
     3181 gataatcatc gtcccgccaa tcagttcgcc aatgtgcatc cccacggcgg tgatcatcgg
     3241 cagcgaggca ttgcgcagga tgtggcgacg ttcggtctgt ttgtcgttca ggccgcgcag
     3301 acgcgcccag gtgacgtgac gctgaccggc gacgtccagc atactggcgc gcagcagccg
     3361 tgcgttaatc gccagcgaca taaaggcaat ggaaaccgca ggcaaaatga tgtgctgcca
     3421 gccgccgtaa cccatcgcgg gtagccattg cagatacacc gaaaacgcca tcaccagtaa
     3481 aaacgccagc cagaagttag gcatcgacac gccgagaaac gcgatgaaac gtacggcgaa
     3541 atccggcaga cgatcgcgat ggcgcgccgc ccagataccg agcggcacag aagtgagcag
     3601 aattaatacc agcgcggcac ctgcaagttc cagcgtggcg ggcaggaagt tcagcatatc
     3661 gtccagcact gggcgttggc tggcgaatga gataccaaag tcaagatgca gcgccttcca
     3721 caaccaggta ccgtactgga cgtacagcgg ctgatccagt ccgagcatgg tgcgggtaga
     3781 ggccagcatc tctggcgtcg gcggcaggtt agacagacgc aaataatcga gcgccgggtc
     3841 gccggtacca aggcgcagca tcagaaaaat gatcaccgag gcggcgagca ccatcgggat
     3901 cagcagcaga aagcggcgta atacgtaacg caacattaag gtttcaccgg tttaatctgt
     3961 tcgaacggaa tttcggtggc gatcggcgcg taggggatgt tacccagctc cggttttgat
     4021 accaccatca ttgagatgta actgataggc agataaaccg cctcgtcatg cagacgggtc
     4081 agaatgtcgc gatacagcgc ctgacgttgc gtttcgtcat gggtcgctag cacttcgccg
     4141 atctctttat caatcagcgg tttgtcggct aatccttgct gtgcctggaa gtcagcgtgt
     4201 gacggtacgc gcattgaact caggaaggcg tgtgggtcgt atggcgcgcc ccaggtgcgg
     4261 tggaaaatca tgccaaaacg accgtcgcgc tgacgagcat agatactgct ctcttcttcg
     4321 ccaatcagcg agacatctgc gccaatctgg cgcatatcag cctgaatgat ttccgccatc
     4381 gatttgctta acgcatcggt gccgatgaac gacagttcaa tgcgcagcgg ctgaccattt
     4441 ttctcgcgga tgtctttgcc cgcaggcagc gtccaaccgg ctttttccag caacgctttc
     4501 gctttctgcg gatcgtactg gcgcggtttc aggccgagat tggcgtaggg cacagaaggg
     4561 gcaaacaggg tgtcggcgac ctgctgggtg ccatacaacg cgttatcaat cagcgatttt
     4621 ttgtttaccg cgtaattaag agcttcacgt actgccagct cattggtggg ggctttggcg
     4681 gtattaagcg ccagcatcac ggtttcgatc ggctgtgaca gttgggtatg gtaagccggg
     4741 ttctggctaa agcgggcgaa ggtatcgagc ggtaataacc cttcgtttcc gtacagcagg
     4801 tcgatatcgc cagtttcaaa cgccaccgcg cgggtagtcg ggtccgggat gacgttaaaa
     4861 gtgatcttct taattgccgg cttttcgccc cagtagtttt cgttacggac gaagacatcg
     4921 tactgattca gtttcgattc ctgcaaaacc cacggcccgg tgccaatcgg cgctttaatc
     4981 ccgttcatgg tttcatggtt tttaaactgc gagggggcga taaagcggaa agggcgcggc
     5041 agggccagtt cttgcaggaa aggatagtag gcgcttttca gggtgatttg cagctctgtt
     5101 ttactgagtg ctttgacatc aacaatctgg tttgccagct ccagccaggc gtgacgttga
     5161 cggttatcga gcactgcgcg gaagttttct gccgccgcct ctgcatcaaa cggttcgccg
     5221 ttagagaatt tcacgtcatc acgcagggtg aaggtccagg ttttaccatc ttctgaatga
     5281 gtccagcttt ttgccagcca cgggatcacc gaaccatcag cctgatattt caccagcggt
     5341 tcataaacca tgctctgggc gaacatctgg ttaggcgtgt aaaggtgcgg gtttagtggc
     5401 ccgacattca ccggccaggc ggtggtgatt tcatctggtg cagcggcatg gacgataaaa
     5461 gacgcacaag ccagcagcgc aaatagagtg cggcggagtg tggagagcat ggtaacccca
     5521 atggattaaa atagatggcg gaaataagta tgacgatttt aagtattcgt catactgatt
     5581 acctgttctg gatcaataga tgggcagtcg ggtgggcgga tcagttaact gaatcgatcc
     5641 attgcacact gtcggcggtg agcgtaaagg gggtgggggt gcagatcgca aggcttaaat
     5701 tttctagctg acaatggctg actgacagcg aggagtgata ggtgctatct acgctgacga
     5761 tttgccaggc gctgccgcca cgctgtttaa cgatggcttc tttgcgcgtc cagatgcgcc
     5821 agaacatttc cagttgctga tcaggatgca ctgcgtccat ttcagcgtgt tccccgaggc
     5881 tgaacacggc gttcgccagc cagcgccagt tggcgcgcgg gcgaatcact tcgatatcgc
     5941 agccgacttc gccttcatca ctcaacagca gggcgatatc gtcaccgcta tggcttaagt
     6001 tgaaccatag cggcgtttcc ggcgcgaatg caggtttgcc ttgttcgcca tagatgatct
     6061 ccggtagcgg ggaaagcgtg tgcgaaagca atgcacgccc cgccagccag cgttcgcgtc
     6121 gtggaccttg cggtgcttgc tcgcgtaaac ccggtggcag tggagctgcg cttaaggtcg
     6181 aaactttccc cagaactatc cgatacatat cagggccaac gtttaatgat gatggatgta
     6241 ttaatgccgc caaaggcaaa gttgttactc tgcaaaaact cacaatcaac cttgcgggct
     6301 tcatgcatga tgtaatccag cgcgccacaa ttcgggtctg gtttattcag gtttagcgta
     6361 ggcgcaaacc aaccttcgcg catcatttgc agactcatcc aggcttcgag cgcgccacag
     6421 gcaccaagcg tatgaccaaa ataacttttc agcgaggaga gcggcacatt atcaccgtaa
     6481 atggttgctg tcgccagact ttctgccata tcaccgcgat cggtcgccgt accatgcgct
     6541 gaaatatagc cgatatcttg tgcgcttaac ccggcgattt tcagcgattg ctccatacaa
     6601 tactgcatgg tttcgcgctg cggctgggta atatgtgccg cgtcgcagtt agtcgcaaag
     6661 ccgacgattt caccgtaaat ggtcgcgccg cgcgctttgg cgtgctccag ttcttcgaga
     6721 atgagcgtac ctgcgccttc accaatcacc agcccgtcgc ggttttcatc gaacggtgaa
     6781 ggtgtggttt tcggcgcatc attgtgctgg ctggtggcga aaagcgtatc aaataccgct
     6841 gcttctgaag gacataactc ttcggcacca ccagcaacca tcaccgtttg ataaccgtgg
     6901 cgaatggctt cccacgcata accgattgcc tggctgccgg aagtacaggc gctggaggtt
     6961 gggatcaccc gcccgcgcag gccgaaaaag aggccagtat taacggcggt ggtgtgcggc
     7021 atcatctgca cataggtggt gccagtgatg ttattggtgt gcttttccgt cagcatggtg
     7081 gcgaactcgc ttaccgggcc ggtactgccg gtcgatgaac cgtacgcaat gcccgtttcg
     7141 ccgctggtta atatcggatc gccaatcaac cctgcctgct ctaacgccag ctcgctggca
     7201 cgggtcgaca tttgcgacac gcggcccatc gcacggatac gcttgcgggt gtagtgctcc
     7261 ggtagcgtga aatcatcaac aggcgcgcca agcagggtgt gcaggccatc gtagacctgc
     7321 cactccggca ttttgcgcac cgcattttca tacgccagta agcgtgcaga aacgtcctgc
     7381 cagttttcac caaaggcagt cacgccgccc attcctgtaa tcactacgcg acgtgtcata
     7441 acatccctcc gttgatggaa ataacctggc gggtgacgta acccgcaata tcagacatta
     7501 aataactggc aagcccggca acttcttccg cctggcccat acgtttcatg gggatcatcg
     7561 acattgcctc tttcagagcg ctctcttcca tttcgatcat cccggtatca ataagccccg
     7621 gcgcgatgca gttgacggtg attttgcgct tcgcgagttc gatagccagc gcttttgttg
     7681 cgccgataat tccggctttg gcagcgctgt aattcacctg gccgcgattg cccattacgc
     7741 cggaaaccga ggatagcgtg atgatgcgcc caccctggcg tgcgccgatc atcggcataa
     7801 tgcacggctg aatgacgttg tagaaactgt cgaggttggt gtggatcacc gcatcccagt
     7861 cgtcatcgct taaagccgga aaagcggcat cgcgggcgat cccggcgtta ctgaccacgc
     7921 cgtaccaggc accgtgttgc gcgatctcat gctccagcac ttcccgacac tgttcgcggt
     7981 ttgcgacgtc gaaactgagc aaacgcccgt tacccccgtt ggcgacaatg gcattcagcg
     8041 tttcctgagc gcctgtggcg tcacggtgat agtgaacgcc aatgttaaag ccgtctgccg
     8101 ccagttggca ggcgatggcg cgaccaatgc ctttgctggc accagtaacc agaactgaac
     8161 gactcatcag gacgctcctt gttgaaacag ggtggtaagt tcttccgcgg tcggctggaa
     8221 ggtgttaatg cggcccgtcg cctcgccgtc gttgatgctg cactcaaaac tgccgaagcg
     8281 atcgtcctgc atcagcagtt tgacggtaat ggtcagcgtc tggcctgcgg gcagaatgcc
     8341 agcggcgcaa agcaattcac gcgcgcccag tatcatccct aactcaatac tgtttttgcc
     8401 ctgctggtgg cgatgccagc cagaccagac gccgacggtt tgcgccatca gttcaagggc
     8461 aaaccagccg gggagattgc cgtccgggtc gagaaacggt gccagtacgc cactgggcga
     8521 aaccgtcacg cggcacaccg cgctgtcatc gctgacgctc accacgtctt ccagcagtaa
     8581 catgggcgcg tcgtggggca gataagcgcc gggggataaa tagtggctca cgaaaccctc
     8641 ccgagcagaa tgctggcgtt attgccgcca aaggcgaagg aattggacag gatcaccgga
     8701 cgtgccagcg gctgcggttt ttcgataatg ccgcagggtg gcagcgtggg atcgcgtggc
     8761 gacaggctga aatcttgggc gggcagcggt aaatcacgct gtaagatcaa catactgatt
     8821 gctgcttcgg taatgcccgc cgcgcccagc gtgtggccgg tcaaatgttt ggtggagctg
     8881 cacggtacgc gttcgccaaa cagggcgtta accaccatcg attcaatctg gtcgttgagt
     8941 tgcgtggcgg tgccgtgcag gttgatatag ccgacgtcat ctggcgtaag ctgcgcatcg
     9001 gtcagtgcct gattaatggc gcggatcgcc ccttcgccct gcggatgcgg ggccgaaata
     9061 tggtaagcgt cactcgattc accaacgccc agcaatgcga tgagctgcgg ttcccgggtc
     9121 agcagcatta gccccgcccc ttcaccaatg gtgatacctg cgcggtcacg accaaacggc
     9181 tgacataagg ttggcgacag cgattcgagg ctgtgaaaac cgttaatcgg catccggctc
     9241 agggtgtctg cgccacccac aatggcggca tcgaccagcc ccgcctcgat aagcctgcgc
     9301 ccgctgatta tcgctctggc gctggaagag caggcggttg aaagggtata ggccgggccg
     9361 tcgagcgcca gccagtggct gagaaaacgc gacggatcgc cgagttcctg ttgcgcgtag
     9421 tgccatgtgg ggctcggctg cccgtgggtg cgcaggttaa cgtgcgtgtc gccttcatgc
     9481 aggccggagg tgctggtacc aagaacaatc gcaatgcgct cgcggccata tttcgcaatg
     9541 gcgtcatcaa tctgcggctg gatctgcgcc agcgccgcca gcaggatctg attgttgcgc
     9601 gatcggtgcg cagcgaattt ttccgggata accggaagct cgccatccac gccagccagg
     9661 accgcctgcg gatgcccctg caaccagcct gcgcgtgggc gcattccggg ggcgacaccg
     9721 cgagccaggt tggttgcgat ttcatcaagg ttatttccca gggcgttgat catgccgacg
     9781 gcggaaatat aaatcatatc agtcacctaa gtattgaatg gtgatgtggt atttaaagac
     9841 atgctgctca atgctgattg gcacgcgttt tccctggcga ttcagatagg tgatttccgt
     9901 gaccagtttg ccgctggcgt tacgcagctc gcgtttgtca ccgttgtcgc gaagtgtcca
     9961 tcctgcggga agttgcggtt gccaggcgct aatcggccag tggctgagca tcacgtcagc
    10021 cagtacctga cttgcgggcg gtaactgtgg gacgacgatg gattgctcgg cgcgtagccc
    10081 ttgcgcatcg taggtcacca gaaacaggcg aatgccgacc gacgacagcc cggcaagggt
    10141 gattttctga tcgtcggcgt taagcatcac taacagcgac tgggttttgc cgttgaagct
    10201 gccggtcaac agttgctgcg aattgactgc gggtgaaatc cctggtgccg gcagcgtgat
    10261 gcgcgtgcca ggttgcagcc acgcctgcgg gcggccttcc tgttctggtt gcgagtggct
    10321 acagccggtg aggataagcg tagcggtcag ggcgaacgct cgccagaatg tggatttgat
    10381 catttttttg ttctcttttt atcgggcata gccagcggcg aaagcaggaa ggcggtgaaa
    10441 ataccgctca ccagcacaat gccaaaactg ctgatggcct gggtggcgct gaataccaac
    10501 attcccagcg tcagcaaggt ggtgagcatt gccagtgtga tcgccagtag cgaagttaat
    10561 ggtgtgccgc gcggattact gaaaaacagc gtgtaattga tgccgatgcc aagcaccagc
    10621 accagcgcca gcagcgaaaa gagattcacc gcctgcccgc tcatcgccag caccgccaga
    10681 ccgcagccca gcgaaagcac cgaaggcacc aggctgataa gccctttgcg ccagccgaga
    10741 cgggccactg cgccgcaggc aatcaccgcc agcgccacca gcaacaagcc ggttaacaca
    10801 taacggtaaa gtgcgaacaa ttcatcaaag gtgtttttgc gatcaaccca ggcaatgcca
    10861 caagggtaat agctggagat ttcctgtagc gccgcgctat ttttaacccc ttcaaccggc
    10921 accagcacgc cgctttcgcc attttccagc gtcagccaca gcagacgcca gccttcactg
    10981 gcggggcttg ccagccattc atcaaccttc accggcatgg cgttgagatc cgggttcact
    11041 gttgtcagcc cggcattttg cagtgctttt gttactgtcg gggccgctgt tgtcagcaga
    11101 tgtaaatctt cctcctgccg cgccagggag ttcagcggaa tggtgcggta gttgctgata
    11161 agcccctctt ttttcgcatt ctccagcgag gtggtaaatt tctccagtcg ccgcaatgtt
    11221 tgctgtgggg agtcgccgta aaccacgaac catttttgat cgacgctctg cccggtcaga
    11281 gcggtgatgg ctttctcctg ggccagaata tgctgcggta gcgcctgtaa ctgcgagata
    11341 tcgtcatcga cgcgcagcat tgacatcccc gccagcgaaa acagcgccag cacgacgggc
    11401 agaccccgcg acagtttttt attgcgccgc cacgctgcca gccagcgtag catcagcgcc
    11461 atcgccggaa ccggacgcac cggcaggcca cggcacagcc acggatgcca gaacaggacg
    11521 gtcagacagg aggcgctcaa cccgacggcg gcaaaaatcg ccatctggcg aatgccgggg
    11581 aagggggcga gcatcataat cagatacgcc gccacggtgg tgagcagcgc cagcagcaag
    11641 gcattgcgca ctttcgccag gctttgccac ggtgaaacgt cgttgccgtg caccatccgc
    11701 tcggtgagat aatagagcgt gtagtcagcg gaaatgccga taacgctcat gctcatcacc
    11761 agcgtcatca ggtgtaattc accgaaaatc aataaagtgg cgaccgttcc cgccagcgcg
    11821 ccgatgccga tggaaatcac gcaaagcagc aacgggcgca aagagcggaa caccgccaca
    11881 atcagcaata tcacccccag cagcgttgcc acgcccaggg ttgagatatc ctgcttcgcc
    11941 tgttggctgg cgtaatcgct gtaaaacacc gtgccgcgcg aaagcagctg cgcctgcggg
    12001 taacgcgttt tcagatcctt ttccagcgta ttcagggtcg tgatgagctg gtgggtttgc
    12061 tgcatatcaa acgacgatcc cgccagttca ccgtgcagca gataccagta gttgccctgg
    12121 ggatcctgcg tcaccagcca gccgtccatc agccgcaaac gctggccgtt tttcgccatc
    12181 gccagctgcg agccgcgcat taacatcagc ggatcgtttt gcagctcctt gccgcttacc
    12241 ccggagaacg cagaataaag ctgggagagg atccactgcg cctgcgcttc gccgccgttt
    12301 tgcaggcgcg cgcgggtgtc ggggtcaatc aggccgttgc ggtgctgcca gaaaaacgct
    12361 ccccacgctt gctggctggc ggcatccatt ggccctttaa cgtcgccgag cgcagcggat
    12421 ttttgcagca gcgtcagcca ctcctgagcg accctgggat tagcctcttt accggggctg
    12481 accagccaca ccagttggcg gtccagacgc tgcataaagc cgtcattcag cgctggggga
    12541 atatcgccca tagcctgttt gggtaacata gccagcacgc tactgttcag ccgtgattgc
    12601 ggcagcagga tcagcaacgc cgccgccatg accaggcaga ctagccccca taacagcgcg
    12661 gggcgtttac tgggcggcaa aacgttggcg ttcgtcatcg gtcagttgcg ctggcgtcag
    12721 ttgatgttgg gtaagagcaa tatcggtgcg atcgccctgt ttatcattaa gttgaatgct
    12781 ctccagatag gttttcccgg cgagatcgat ggtgttgaaa attttatcca gcggcgtggt
    12841 ggtcggcgtc aggcgcagcg tccagcggcc ttcgcctttg tcagcaaatt cgacgcggaa
    12901 gttttgttcc agcactttgc gatcggcctg gaacagcgcg cgcagcaggt ggttaaactg
    12961 gaacatctgc gggttgtttt ctgccgtgat gatttgcggc ggctgaccgt tgatcacctg
    13021 caccatgcgt ttatcatcca gcaatagctg catggggaac ggtgaggttt gatcccacaa
    13081 taacccctgg tcgcgggcga tcaacatctg accctgagat cgcagcggct gcggcagatc
    13141 tttaatcgtc cgggtttgat caaaatgggc gcggatcacc ggttgttcgg taaagcgttg
    13201 ctgaagatcg tccagggtca gggcgctcac aaacgggcta atcagcagcg ccagcagcgg
    13261 taaaaatttc atggtttgac tcccatgcgt tcaaacagaa tatcggggct gacaaagcac
    13321 agttcgcgac tttgctcgtc gaccgcgacc tgaatggtgt atccggtggt ggcgcgtttg
    13381 cccgtttcgg catcgaaaat ctgatagcca atccgcagac ggttttcgaa ctcttcgata
    13441 tgcgcacgca cgcggatgcg ttgctcaaag gtgagggcgt ggcgatattt cacccgcgca
    13501 tcgaccactg gccacaaata accggactct ttcatctgcc gatagccgta attgaactgg
    13561 ttgagcagcg cctcgcgggc cacttcaaag tagcgaaaat agttaccgtg ccaggccacg
    13621 cccatcatat cgacgtcgtg aaagggaatg gtcagctcga cttcagcggt aaatcgggga
    13681 tcgttaagca ccctttactc cttgtcctga atttccggca gttgccagaa atcgaaaaaa
    13741 ttaaaccagt cgaggggcga ctggagcgcg taatgttcca gacgcgcggc gtagtggtcg
    13801 atagcgtttt gcagcgcctg ttggcgttcg ccacgcggca acagcagcgg gtcggcaaag
    13861 gtttcgcagt gaatatgcag cttgccatgc tggcgtaggg cgaaaatcag attcaccggg
    13921 cagcgcaaaa tagaggcgag aataaacggc ccctgtggga agggcgcagg ctggcccata
    13981 aacggactcc agcagacgcg ccagtcgccg ccgcgttgcg gggtgacggc gatgcggtca
    14041 ccgacaatcg ccacccattc gccgttatcc agcttctctt tcagcaggat ggcggtttcc
    14101 gggccgatat ctgttaccgg catcaggtta atgcctgcct gaggagccat ctcctgcatt
    14161 atctgtttaa agcgttgggc gttttcgcta aacaccagcg cgttaatggt tttgtagccc
    14221 tgaatttttg ccagcgcccg gcacacttcc acatcgccaa gatgcgaggc cagcagcaat
    14281 ttgccgcgcg gatcgcggac gtcaagcgcc gcttctgcgc ctggcgcaaa cagcacatca
    14341 cgccccagtt gtagctcgcc gcgccagctg gcgattttgt cgagcatggc attaccgaaa
    14401 cgtagaaagt gctgatagct gttgagtttt gccgcctgtg gttgatgctg tcgtacacgg
    14461 gcgagccagt cttgcgacgc tttgcgcgca cgagaagcag tgagccagta gacgcccacc
    14521 accgggtaaa gcagcgcgct aaacgccgtt cttcccagca gacgccagac cagcagcatc
    14581 aggcgcattc cccataatcc tttcacttca ctctgccgcg cccagtggct ggaagagcgg
    14641 cgcatcagca gtgaaggaat acgcggcaac atgccgaaaa acagacgcgt gtgcatgagc
    14701 gagatgcgga cgttatcttt cagggcatca aaatgcgaaa gcccgtccag tggataggtg
    14761 acgcgggtcg gcacgaaata gctggtgttt ccctgccagt agaggcgcac catcacttca
    14821 gtgtcgaaat ccatccgctt gccgatggtg gcgtgttttg ccagttgcag cgttggcgca
    14881 accggataaa cgcgaaagcc gcacatgctg tctttcagtt gcagggagag cgtttcgatc
    14941 catacccaga cgtgggtgac ccagcgcccg taaagccgtg agcgggggat ggagtcatcg
    15001 taaattggct ggccggagat cagcgccgca gggtgttgtt cagcgagtgc cagcagtttg
    15061 gggatatctt caatcgcatg ttgaccgtca gcatccacct gcaccgcatg gctgaaccct
    15121 gcgtctgccg ctgcctgtaa gccacgcatc actgccgcgc ctttacctgc gttttctgct
    15181 aagcgaatta aggtcacgcc aggctgttcg gcaagcagac tgtccagttg ctgttgcgtg
    15241 gcggcatcgc tgccgtcatc cactacaata cagggcagat taaatggctt aagacgcgcc
    15301 agcacgcccg gcatcatcgc gccgtggttg tagcagggta tcaacacgca gggagaaaag
    15361 tttaccgaca taggcggatc ttcccgctac tggcggtgtg gcgggcgtca ccgtcgtgac
    15421 gctgatagct aaagctcaga atctggcgtt cttcctgcca gttaagcgcg agcgtgacgc
    15481 ggttttccgg cagcaacgga gactggaatt ttatgttctg aatggagtga aaacgccagc
    15541 caggcgcgag cagattgagc gcgtagctca tcgcccagtc gatttgcgca actcctggga
    15601 gtaatggttg cacggcaaaa tgaccgcgaa accagaacag atccgccctg agatgcagaa
    15661 caatttcaag gtgattcgtt tgtgcctgat ggcgctcgat ttcatggggc ttcatgaaat
    15721 aactcctgta attgcgcata gacacgcttg ttcatactgt ttactggcat ttcatcaata
    15781 acgcgccagt aacgcggaat agcaaccggc tccagcatcg ggtgcagtaa tcgccgccag
    15841 gcctgctcct ggctatgccc gccgccgttc tgccattgca gatgagcttc atcatccagc
    15901 accagcaaaa cgccaatgca ctgacgcccg ccgcgtgtaa caggaactgc cgccgcctcg
    15961 cgaatgccgt ccagtgccag cagacgttgt tccacttcct gcaaagaaat acgtttttct
    16021 tcaatcttaa cgatgcgccc acgacgcccc atcagatgga actgaccatc ttcgctaaac
    16081 tgtaagatat cgtcgagtaa caggccgtta tcttccgcta tcagcggtga aaagaggcga
    16141 aaggcatcgc cttcggcctg gaactgcata cctgggaggg gtagccagcg gcgttgctct
    16201 tgttcgcggt agcgccaggc gatgacgccg gtttccgtgc tgccgtagat ttcatcaggc
    16261 cagacgcgca gccagcttgc ggtgtgttgt acatcctgcc acggcagctc accgccagca
    16321 gagagaagca cctgaacggg tggcggagaa agctgggtat ccaggcgctt caaaaatgcc
    16381 gggctgctga tgaagatata gcgatgctga tgactcaacg cggcaaactg ttcgacatac
    16441 cagagcatgg cggcgtgcag cggtaatccc aacgccatgg gcaggaaaac gcgaaaagtc
    16501 aggccgtaca aatgctgcgg caatacggaa ccgacgacac gacaacccgc cagacgtgcg
    16561 cctaatcgtt cagccagcag ttctgcttca cgatccagca aatgaacagg tttaatcacc
    16621 cgtttcggct gtcccgtgga accggaggta aacagctcga taaaagcctc tggcgctata
    16681 gcggcaaagg tgaaggattg tgtcgcgatt tgtggagaac ttgctaccaa cagcaatgaa
    16741 ccttgccagt taagttcgct gtcgctaagc acaccatcga acagttcacg ttgctcgtta
    16801 agctggatga cacggttatg ccccggaagc accggtgttt ttccggcgtg tagcgtggcg
    16861 agcagggcaa cgataaacag atagctgttc tcaaagcaca gcgcccagcg ttcacccggc
    16921 tgttgctgca agcggcagat aagttgcgcg acatcgtgac gcaaatcgcc cagcgtccag
    16981 gtactttcgt tcagccacgc aatgggtgtg tcatcagggc gtggcgcagt cagccagcgt
    17041 gcaagcggta aggtctgctt catgcgcgat ctcttttcat aacctggcgg cgcaccagcc
    17101 attcaccagc cattaacgtc cccatcaaca aataagcaat catgccgttc caggcggtcc
    17161 ataacgacat atcggcataa agcgcggtaa agagggcaat tccaccgtta atgatgaaaa
    17221 agccgcacca gatttgcgtg acgtggcggg tatagcgcac gcccttttcc gggaggtctg
    17281 gctcctgcaa ccgcgccagc cgctcaacta tcggcattgc cgaccacagc gaaccgccaa
    17341 aaaccgccag catgaccgcg ttaaccacta ccgggtaaaa cagcagtagc tggtgcgttt
    17401 tcaacataaa gctggcgaca cataatgcga tgcccacgac agcgacgatt tgtgtgacgg
    17461 cttgtaaagg ccccgcctgg cgacggggct ggcgcagacg caacaaaagc agcaacgcca
    17521 ttagcggtaa cagccagtgc aggccgttat gcgctaagcc aaaccaaatc acaaacggcc
    17581 aggcgagcag caataatcca gtcagcagct taatcatcgg tagcgagcga atgcccgaca
    17641 tcagatcagg cttcttgtag caggcgttct acagcctcga cgacatcctg gactgtacgc
    17701 actgctttaa actcttccgg cttgattttc ttaccggttt tcttttgcag gtgcacaatc
    17761 atgtcaacgg cgtcgatgct gtccagttcc agatcttcgt acaggcgcgc ttcaggtttg
    17821 atgtccttcg gatcgatttc aaacagctta accagcaggg ctgagacttc ctgatagacg
    17881 gtttgttgtt ctgtcatgat ggctttcctc aggcacgttg cgcagcaatg aaagaggcca
    17941 gagtggcgac ggagaaaaag tgctgacgca tctcttcgct ttccgcagag agcacgatgc
    18001 cgtactcatt ttttactgcc agtcccagtt ccagggcatc gatagagtcc agccctaaac
    18061 cgtcgccaaa cagcggcgcg tcggtatcaa tatcatcagg cgtcaactcg tccagattga
    18121 gggtgctgat gatgaggttt ttaatttcca gataaagcgc ttgcattatt tattcctgag
    18181 agaggtaaac aaccttgttg taattgaagc aaaagatgcc ggtttaactg ccttgctgcc
    18241 agcgccggtt cttgtgaagt tgcatcgtaa aatttattga tttcaacccg accatggact
    18301 gcaaccgtaa aaaacggccg gtcaggtgga acgtcatacc atttactttg tttatccagc
    18361 agatgctcgc tacagccgat tgtcacgatg cgtaaatcgc tggcgcagcg cacggcaata
    18421 ttcgccgccc cgcgctgcag cgtcattttt tcaccgggct tcgttcgtgt gccttcgggg
    18481 aaaatcagga tagtgtcacc ctgtgccagt cgctgttggc agcggggtaa cagggtttca
    18541 gcttcgctgt tgattaagta atctgccgcc cgcacgacgc cgccaagaaa aggatttttt
    18601 aacagtgcgc ttttcaccag gcagtcagtt tctggcatga ctgacgccag cagcacgtaa
    18661 tcgatcagcg tgggatgatt ggctacgacg aggcaaccgc gttcctgacg taaaatttca
    18721 gcgccatcaa tacgataatc cagcacgcca agccctttcg cgacagttaa aaacaggcga
    18781 aaactggcgg caatgctgcg acgcgccaaa cgacggcggc gggaagtatc ccacaccaaa
    18841 actaacaata tgttaaacca cacgacagaa agcagcaggc cgccgagacc aaacagggca
    18901 aaacataacc cggtcatgac cagtcgccag gtccactcca gacgtgacat cagcttactc
    18961 atctgtggct ccagcgccat tgcacgcgct cgccaggcag tgaaaatgtg tctacatttt
    19021 gtaaatagtg ctgtaaaaac aacatgcttt gcggcagtgt tgtttcattg cccgcaatgg
    19081 ctgactgcgt ttcacattgc cagtcatcgc cagcttcaat caccagcgcg acggcgtacg
    19141 gccaggtcgg catttctgca ggcagttgtg ggtgataaaa ctcgggcagg aaaccgtcaa
    19201 aatcgaccat cagcacgcgc tggtatcccg cttgtaacag gctaagcact tcacacaaac
    19261 cttgctggaa agtgtcgcga ccagcagaaa gcgatgacga aacgatcggc tgtttggccg
    19321 caatggtcag gttacccacg gaagagttgt gtactgaaag tgcaaagtcg gtcggagaaa
    19381 gcgcctgttc ggttgccagt gcatgaacaa tgcggtaatt gcgctccagt tcgccatgac
    19441 gactggtata caaaacggcg tctggctgat ggcggcgtag cattgccaga ccgcattcaa
    19501 cagccagttt gctgcctgaa ctgaggcggc gggcggtcat cattggcagt tcacttaatt
    19561 ttgccagcgg cgcggcaggg tcaatcgccc acggctggcg cgaccatgct tgccattgct
    19621 gcgcttcgct aagtccaggc gccagtgcct gccagtcggt aatatttaat gtaaatttca
    19681 tcaacgcgcg cccgcggtaa accaaaatta ttaagtgacg attatttctt ttggcatatc
    19741 aaaagtgtat gcccaacgcc taaattatcg tggcgctctg ctacctggaa tcctgcttta
    19801 ttcagatagt cgtaaaattt ctctgcacta tagaaacggc tgttaccgtt tgccatgcag
    19861 gtaaaataaa gtgaggacgc gttcaggcta aatgatgctg cttcaaaccg ctgcgcatcc
    19921 cagaacaact ccatgatgca aacttttgcg ccaggcttca ttacgctggc aattttgctg
    19981 agtatgctaa ttatttgttc gggtgaaaaa cagtctaaaa attggctcat ccaccagata
    20041 tcagcctcgc ccggtaatgg ggcatcgctt agcatatcca ccgcatggaa atctatgcga
    20101 tcagaaaaac ctgcattcgc gatattttct ttcgctagta cgatttgttg tggcagatct
    20161 aataacgtca cggcgatgtt ttcattgtat ttgcagcaac gcaaagccca cttacccgta
    20221 ttgccgccta catcgtacaa tgttgtgggg ttgttggcga atacataagg cagtgcggcg
    20281 ttaaatgcgc catctgaata gtaatgatca aacgcaaacc agctttcacg agcggcgtct
    20341 ggtaattgcg aaagtgccgg atatatcgtc gaccagtcgc caaatacttt taacccggaa
    20401 ggttttcctt cattgagtga atcggcgagg aagaagagcc cctggtaaca gacatcctgg
    20461 gtgaaatcca tattcacgcg agtcatagtg tcatgcagga ggtaatgacc gattttcgcc
    20521 aggtaataaa ttccctcttt acaggtaata attcgcccgc ttaagcccat atctaacaga
    20581 acgctggcag catattcgtt gatatggcta tgttcggtga tatcagccag cgttgcgcca
    20641 tgtttgcctt gccgatccag atatgaaagt acacccgcat tacgtaggca gagtgctgtt
    20701 tgaaaaagca taggggcaaa ggcaatgcgt tgagcttcgg taatcgcatc cagtgcgcta
    20761 agagaatcct gttcgtacat gttgggcaca acctgttaag agggaattaa attattatcg
    20821 gaccgcaacg gcccgacttt aggaagaatt ataaatctgc gcccgctgac aaattcagtt
    20881 gaatatcttt gtcgagattg cggacccagc gattgtagtt tttatgaatt tgtccgcctg
    20941 acgcttgtag gttctggcta ttttcataat tgatatcgta gctggatgcc gtgtagttaa
    21001 tgcgtatttc cgctgaatga tcgcgtgact gttgacgtgc tttaattatg ccttgtccgg
    21061 cttcggacat aatccatttg cgttcaacgc cagctttcag aatcgctttt ttcacttgtt
    21121 cctgggtatg accagcgctg acggttgagt gaacctgatc aattggcgca gtacgcgcgc
    21181 aacctgctaa agcacccaca acagcaatag ccgcaaacca tttcatgtaa tgtctcatta
    21241 gaaccccatt aaagtattgt ttttacgagt aaagcaagat ttaactgtaa ttgctggcat
    21301 cactattctt tatttagaga aaaaattact tgtgagataa gtcagtaagt gcacatcaga
    21361 gtaaaaatct ctgaaatgac taaattactg ccagcaggga ccaactataa gtgagtgaga
    21421 aaaatgaggc aataaaaata acacaatgtt atcagtgcat tctttgagaa ggatttaatc
    21481 gctattcggc gtttatatat atttgactta aggaatggtt tttttaaatt aattttctga
    21541 ataatgtttc atcatttcct caaatcaaat ggccactgtt agataaacgg tggccattgc
    21601 ctgctcagga ggtgctatta acccggtaac cgacttttcg gtctgcccgg ccccagtaaa
    21661 atcgccagtt tgctgccgcc tttcgtcgtt tccatccaga ttttacacac gctggttaat
    21721 ggcaccgaaa gtagcatccc taccgggccg agcagccatc cccaaattaa caatgaaaga
    21781 aataccacca tggtggacat ccccaggcga tggcccatca tccgtggttc taaaatattg
    21841 ccgatgacca tatggaccac taaaaacaat gcgccgacca gaatacattc gtaaacacca
    21901 ttaaacagca gcacctgaat cattggcggt acggcggaaa ttaccgcgcc gatattgggc
    21961 acgtagttaa gcaaaaacgc cagtactgcc cacatcagcg caaactgcac ccccatcagc
    22021 tccagcccca gccagacgat gacacctgtc cataaactga gtagcgtctt taacgccaga
    22081 taatgcgaaa cgcctttaag tgcgcggtgt aatcctgcga tgtgaatctg tgggttattc
    22141 aatgcaaaac gcattttgta agggacgtgg cgcacttcaa acagcataaa aactacggtc
    22201 atcaccagca aaagcacgct cgccattgcc ccggaaagcc cggtcattag cgctgtggtg
    22261 aaggtaacca ctttttccga gtccatccgc tgcagcattc gctccggcga catatgcaaa
    22321 ttaagaaaag gcaacatctc ctgcaattta aaaagtttgc gcgtcagctc cttattaaac
    22381 ttcggcagca tagagataaa ttcgttaaac gatgccgcca gtacgccgac cagcgcggtt
    22441 agtgtgatca gcatcaccac caccacaatc gtaatggcaa cggggcgttt tactccccgt
    22501 cgaataaacc aggtaacgag cgggttgagg acgatggcaa aaaacagcgc cagtaacagc
    22561 tgcacaatga tatctgccgc tgcgtgaatg cccgcgagga tcactaccag cgaggccagc
    22621 ttgagcagaa tgtgcatgcc cgttttatcg ggttgagggg tttccattgg ggcttccttg
    22681 tgactttttg tattaagtgt agcgggagtc acgccagcaa tattctgatt cttactgctg
    22741 attttcacgt cagcacgtgg taaaaatgaa acactgttgt aaaaatgtgg tgatcctcat
    22801 gcccgaaccc gtagccgaac ccgcgctaaa cggattgcgc ctgaatttgc gcattgtctc
    22861 cattgtcatg tttaacttcg ccagctacct caccatcggg ttgccgctcg ctgtattacc
    22921 gggctatgtc catgatgtga tgggctttag cgctttctgg gcaggattgg ttatcagcct
    22981 gcaatatttt gccaccttgc tgagccgtcc tcatgccgga cgttacgccg atttgctggg
    23041 ccccaaaaag attgtcgtct tcggtttatg cggctgcttt ttgagcggtc tgggatatct
    23101 gacggcagga ttaaccgcca gtctgcccgt catcagcctg ttattacttt gcctgggacg
    23161 cgtcatcctt gggattgggc aaagttttgc cggaacggga tcgaccctgt ggggcgttgg
    23221 cgtggttggc tcgctgcata tcggacgggt gatttcgtgg aacggtattg tcacttatgg
    23281 ggcgatggcg atgggtgcgc cgttaggtgt cgtgttttat cactggggcg gcttgcaggc
    23341 gttagcgtta atcattatgg ccgtggcgct ggtggccatt ttgttggcga tcccgcgtcc
    23401 gacggtaaaa gccagtaaag gcaaaccgct gccgtttcgc gcggtgcttg ggcgcgtctg
    23461 gctgtacggt atggcgctgg cgttggcttc cgccggattt ggcgtcatcg ccacctttat
    23521 cacgctgttt tatgacgcta aaggttggga cggtgcggct ttcgcgctga cgctgtttag
    23581 ctgtgcgttt gtcggtacgc gtttgttatt ccctaacggc attaaccgta tcggcggctt
    23641 aaacgtagcg atgatttgct ttagcgttga gataatcggc ctgctactgg ttggcgtggc
    23701 gactatgccg tggatggcga aaatcggcgt cttactggcg ggggccgggt tttcgctggt
    23761 gttcccggca ttgggtgtag tggcggtaaa agcggttccg cagcaaaatc agggggcggc
    23821 gctggcaact tacaccgtat ttatggattt atcgcttggc gtgactggac cactggctgg
    23881 gctggtgatg agctgggcgg gcgtaccggt gatttatctg gcggcggcgg gactggtcgc
    23941 aatcgcgtta ttactgacgt ggcgattaaa aaaacggcct ccggaacacg tccctgaggc
    24001 cgcctcatca tcttaaaact tactgaataa ccagcgtatt aatgatgttt tctgcggtgg
    24061 tctgcgcttt ttgctgatcg tcagctggca gagtaatttg catggtcagc agttgattac
    24121 ccacgttacc cagaataacg gaagagtacg ccgtctggcc tttcgcggag ataatgctgt
    24181 ctaactgctg cattttgtga cctttcagct caatggcttt gttggtcact acttgcagct
    24241 gcggatcgcg gctacgttgc tgatcttcca gacgcttcgc cagcaccgcc agatcttctt
    24301 tcggatcatc gcccatgatg acgatgactg ctttctgccc ggtggcgtcg gaccagacat
    24361 gcatgttatt ggcctgcgtt cccagcttac cgctctggtc ggtcatatcc gctggcagcg
    24421 agaaacttaa cttgccatca agcaggttga cgggattccc ggtagcgtta ctttccgcga
    24481 ccgaaccctg cgccgtagcg ttagtgtctt tatcatcaca ggccgcaagc cccataacca
    24541 gcaggccaat tccgacatat ttaaccagat tgcgcattga cttcttcctt tcgataaacg
    24601 gccataacgg ctcattcatc catcttatca caactctgat aacgaacctt taactcgcct
    24661 gcaaagcgtt gatttcggat ttatctgtca gtcttttcaa cagcatattg agtaatacgc
    24721 catacattgg caggaagaaa acgatactga ttaacacttt gaaacagtaa tcgaccagcg
    24781 cgatttccat ccagtgttca gccataaagg catccgggct acgccagaag gcaatgaaga
    24841 aaaaggccag cgtgtcgctg acgttaccga acagtgtgga cgctgtcggt gccagccacc
    24901 agcggcgact ctgacgcagg cggttaaaaa cgtgcacgtc gaggatctgt cccagcgcgt
    24961 aggccatgaa actggcggtg gcgatacggg cgacaaacag gttgaagtgg gcgagtgcgc
    25021 cgaatccctg ccaggaaccc atatagaata gcgacgagat gacgtaggag atcagcaatg
    25081 ccgggatcat caccgcaaag atgatacgac gggccagcgg tgcgccaaaa atacgtacgg
    25141 tcaggtcggt agcaaggaaa ataaacggga agctaaacgc gccccaggtg gtatggaaac
    25201 caaaaatgga gacgggaagc tgaaccagat agttactgga ggtgatcacc agcagatgaa
    25261 ataacgataa ccagaacaac gccttatagc gttgagtttg cgagaaaacg ttcatattgt
    25321 acctttttga ttaaccattg gggtgaggga acccaatacg tacgacacgt ctgttacctt
    25381 aacataataa cgtgcttacc cttttttcga gccgccgcat gatactgctt tacgttgaca
    25441 atgcaatggt taattttcac gcaatcgtta acctggtttg cttacggact cacagggcgt
    25501 aaactagcgc cgttttttta tgtgatgaga agaaaatgac cgatctcttt tccagccctg
    25561 accacacact cgacgcactt ggcctgcgct gcccggaacc cgtaatgatg gtgcgcaaaa
    25621 cagtgcgcaa tatgcagcct ggtgaaacgt tgctgattat tgctgacgat ccggcgacga
    25681 cccgcgatat tcctgggttt tgtaccttta tggaacacga actggttgct aaagagacgg
    25741 atggactgcc ttatcgttat ttgattcgta aaggcggttg atgggggcag attggcttcg
    25801 atgccgcctt ttcccctcac cctaaccctc tccccagagg agcgagggga ccgatcgcgc
    25861 tcaatgttgc gatcggtttg ccttatctcc tgcgcaacaa tcttaacgca ttcgctgtca
    25921 ccagcaccgt cgcacccgta tctgccagca ccgccagcca tagtccggtc attcctaaca
    25981 gcgtggtgac gaggaagatc cctttcagcc ccagcgcaat agtgatgttc tggcggatat
    26041 tggcgtgagt ggcgcgtgcc agttcaatca tttgcaccag accgcgcagg tggttatggg
    26101 ttaatgctgc gtcggcggtt tccagcgcca cgtctgtgcc gctgcccatt gcaatcccga
    26161 tggcggcagc tttcatcgct ggcgcgtcgt taataccgtc accgaccatc gccagcggcg
    26221 catgttgatt cagctcggtt accgctttga ctttatcttc cggcaacagg cccgctttaa
    26281 actccagccc cagctccccg gcaattgccg ccgctgcgcg tggattatcg ccggtgagga
    26341 tcaccccttt gacgcccagc gcgttcagtt cactgatggc agttgcagca tcggcgcgca
    26401 gggtatcctg taacgcaatg acacccagca cgtcatcgtt acgtactacc agcactaccg
    26461 tttgcccggc gctttccagt tcgttaatta aaccagcaaa tgcatcagcg ggatgtttcc
    26521 cggcagcgca aatcaatacg cgctcaccgt taacctgcgc ttcaatgcca gacccgacca
    26581 gcgcccgctg tgattcggcg gcgggaatgg cgagtgcagc aacctgtgct tcgcgcacga
    26641 tggcctgcgc cagtggatgc gtcgcgcctt gctcgaccgc cgccgccagt gtcagcagtt
    26701 cagattcact aatacccgtt gccggatgaa tcgcggtaac gcgcggttta ccgacggtca
    26761 gcgtaccggt tttatcaaac gccacctggg taacacgacc tagctgctcc agcgccgcac
    26821 cgcctttaat taacgcccca cgacgtgccg ctgctgccag cccggaggta atcgccgcgg
    26881 gcgtggagat aactaacgca cacgggcagc caatcagcag cagcgtcagg cctttataaa
    26941 tccactcttg ccagctggcg gcaaacagca gcggcggcac cagcgttacc agcagggcga
    27001 cggccataat cgctggcgta taaatacggc tgaaacggtc gataaaccgc tcaatgggag
    27061 cgcgacgctc ttcggcttct tcgatcagtt tcagaatccg gtcaatggcg ctggctcccg
    27121 gttcggacaa tacttccagc gttaccagac ggtctacgct ggtggctcct gcaggaactt
    27181 tatcgcccgt tgcgcgctcc accggaatgg attcgccggt cagggcgctt tcatcaaaac
    27241 tggcaaaggg cgagagcagt ttaccgtcgg caggcaaacg cccgcccgcc gcaacttcaa
    27301 tcacatcgcc agggcgcagg ctgttaatcg ccacctcttc ccgctcaccg ttacgcaggc
    27361 gcgtggcggt ttctggtttc agcgccatca acgcgctaac gccctgacgc gcgcggctgg
    27421 cggcccagcc ttccagtcgt tcaccaatca aaaacagcag caacaccatc gcagcttcag
    27481 ccgttgcgcc aataaacagc gcaccaatag cagctacgct cattaacgtt tcaatggcga
    27541 agtaactgcc tgatttgatc agccgtaatg cctgacgagc aatcgggtac agcccaacca
    27601 gcgtggtcgc gataaacgcc agttgaccga acggatgatt aaattgctcc agtccccagc
    27661 tgattgccat catcacgata agcgtaatca gcggcagatt ctctttcagg cgcgattctt
    27721 gtggctcgtc ggcggcctgc tcatcgcgca gggaatagcc cgctttttgc accgcagatt
    27781 caacctgggc acggatgtca ttgtcggcat caaccaccag tttttcggtg gcgaacagta
    27841 cctgcacctg attcacgcct gcaagctggc gcacggcatt ttctaccttg cgcgcacagg
    27901 cggcgcagtc catgccgctg actttccagc tatagcgggt gccggagacg ttttcagaga
    27961 gcgttggcgt gctggaacat gcgccgtcgc agcaacagtc gttggcgttc tgtaccgtgg
    28021 ttagcggttt gaacgcagca aattgagggg ctttcttgcc gtgattgtca ggagtcgaca
    28081 tggcatcctc cggttaagtt ttttctcatt aaccgaagga tacactctgg agtcgactcc
    28141 agagtcaagt tttatcagag atacagcgag cggacgatga ggaagtgacc ggcaaagtag
    28201 caggccgcag caatcgcgtt atccgcacgg aagcgtcggc gatagtggct acccagccag
    28261 acaaagctac tgataaacag caacgaagcg ccgacaaacg cagagagtgc cggagcggtc
    28321 ggacggaaga accacagttc acctgccagc cacaccatca ccagcgtcat gccgataaac
    28381 gtgcagatag gccaacgata ctcttccagc cgcgtccaga taatcgccag taacagcgcg
    28441 cccagaacca gcagtaccaa cggcagaggc cagaagaaag agagcgtcat ctgactggcg
    28501 aaatagatgg tgtacagcag gtgcgagagg aaaaacgcgc cgatggcgta catcagacgt
    28561 tgacgtggca acagggttag cgcatcgccc agcagtgagg cgcacagccc tgccagcacc
    28621 agatagctaa tggcgtcgaa catcggcgct tgccaggcca gtaacagcag gagaagaagg
    28681 gttaacggtt taaacaccca gcgttgccag gttggcccac gatacgatgc atccacagat
    28741 agccatgcgg aaagacagac agcgataaac gaccaaagca tcttagttcc ttgaattcgt
    28801 tattttttcg taggcggaat gcctacggtc ttgcttccag tttagtggcg agggtggacg
    28861 gatgacaaca ccataactgg cagggtatgc ttaattccgc attttccgat gagggataaa
    28921 gatgagcaaa ccacctcttt tctttattgt tatcattggc ttaatcatcg tcgccgcatc
    28981 gtttcgtttt atgcaacagc gacgggaaaa agctgataat gatatggcac cgctccagca
    29041 aaagctggtg gtggtgagca acaagcggga aaaaccgatt aacgatcgcc gttcgcgtca
    29101 gcaggaagtg actccggcag gcaccagtat gcgctatgag gcaagcttca aaccgcaaag
    29161 cggaggaatg gagcagacgt ttcgcctcga cgcccagcag taccacgccc tgacagtggg
    29221 cgataaaggt acgctgagct ataaaggaac gcgctttgtt agctttgttg gcgaacaata
    29281 aggttatttt ttaactttaa atttcttctg ccagaccagc agttcaaaca cgccgaaaag
    29341 gaaaattcgc accttttcgg cggtggtcat ctgcgggcca tctttcggta aagtggattt
    29401 caatagcgcc agctgcatac catgcatcag cacggtaaaa atcagcgcca cgttaacgaa
    29461 aatattcagc gggcgcggga agggatgcac caggttgagg attaaaaatc cccaaacgca
    29521 gagcattaac aaacgtccaa tattaatcag catcactttc tccttgtgct tcgcgttgat
    29581 acagccgata agccacctga cccgccactt tttcccgata caatgaccag tttgccggaa
    29641 cagtgggcag accgttttcg acttcgcttt cgacataaat cagggcttcg tcagccagcc
    29701 agccgttatc ttccagtaaa tttatcgtct cttctaacaa gccacggcgg aacggtggat
    29761 cgacaaacac gatattatgc ggtgtgcctt tttgcgccag gaatgacatc gcgttgctgt
    29821 tcactacgcg tgcattgcct gcttttagtg tcgccagatt cttaattaac tgctgagaaa
    29881 ctgcacgatc catctcaatc aacgtggccc ccgcagcgta gcgtgataac gcttccagcc
    29941 ccagcgcgcc gcttccggcg aagcaatcca gacatttggc gtcaacaatg accggagcca
    30001 gccagttaaa taacgtttcg cgtacgcggt cggtggtggg gcgcagacct gggctatcag
    30061 gaaccgggag tttacggcct cgccactgcc cgccaataat gcggatttgg ccgctgccgg
    30121 aatgattcgg ttttttcatg ataaattgct caatccgcca gataacacat acttgcaggc
    30181 ggtgatgtga attaagttaa gtgatagact atttcatcat ttttttagct gctatgtaca
    30241 tagcgttaac gctgtgccat gaagcaacag cgaggagtgt agtcgcaaat ggcgaaagaa
    30301 aaaaaacgtg gctttttttc ctggctgggc tttggtcaaa aagagcagac cccggaaaaa
    30361 gagacagaag ttcagaatga acaaccggtt gtagaagaaa tcgttcaggc gcaagagcct
    30421 gtgaaggctt cggaacaagc cgttgaagag cagccgcagg cgcatactga agccgaggcg
    30481 gaaacttttg ctgccgacgt tgtggaagtc actgaacagg tagctgaaag tgaaaaagcg
    30541 cagcctgaag cggaagtcgt tgcacagccg gaaccggtcg tagaagaaac gccggagcca
    30601 gtggctatcg aacgtgaaga actgccgttg ccggaagacg tcaacgccga agaggtttcg
    30661 ccagaagagt ggcaggctga agcggaaacc gtagagattg tcgaagcggc ggaagaagaa
    30721 gcggctaaag aagaaattac cgacgaagag ctggaagcac aggcgctggc tgccgaagcg
    30781 gcagaagagg cggtgatggt ggttcctccg gcagaagaag agcagccggt ggaagaaatc
    30841 gctcaggagc aggaaaaacc gaccaaagaa ggttttttcg cgcgcctgaa acgcagcctg
    30901 ttaaaaacca aagaaaatct cggttccgga tttatcagcc tgttccgcgg taaaaaaatc
    30961 gacgatgatc tgtttgagga gctggaagag cagcttttga tcgccgatgt gggtgtggaa
    31021 accacacgta aaattatcac caatctgacg gaaggcgcat cccgcaagca gcttcgtgat
    31081 gccgaggcgc tctatggcct gctgaaagac gagatgggcg agattctggc gaaagtcgat
    31141 gagccgctga atgttgaagg caaagcgccg tttgtgatcc tgatggtggg cgtcaacggt
    31201 gtgggtaaaa ccacgacgat tggtaagctg gcacgtcagt ttgagcagca gggtaaatcg
    31261 gtgatgctgg cggcgggcga tactttccgt gcagcagcgg ttgaacagct tcaggtctgg
    31321 ggtcagcgca acaatattcc ggtgattgcc cagcataccg gtgcggattc cgcctctgtt
    31381 atcttcgacg ccattcaggc agctaaagcg cgtaatatcg acgtcctgat tgccgatacc
    31441 gccggacgtc tgcagaacaa atcgcatctg atggaagagt tgaagaaaat cgtccgcgtg
    31501 atgaagaaac tcgacgctga agcgccgcat gaagttatgc tgactattga tgccagcacc
    31561 gggcagaacg cggtaagcca ggccaaactg ttccacgaag ctgttggctt aaccggcatc
    31621 acgctaacga aactggacgg cacggcgaaa ggcggggtaa ttttctcggt ggctgaccag
    31681 tttggtatcc ctatccgcta cattggtgtc ggcgaacgta ttgaggattt gcgtccgttt
    31741 aaggcggacg actttataga ggcacttttt gcccgagagg attaacaatg attcgctttg
    31801 aacatgtcag caaggcttat ctcggtggga gacaggcgct gcagggcgtt acgttccata
    31861 tgcagccggg tgagatggcg tttctgaccg gtcattccgg cgcagggaaa agtaccctcc
    31921 tgaagctgat ctgtgggatt gagcggccca gcgccgggaa aatctggttt agcggccatg
    31981 acatcacgcg tctgaaaaac cgtgaagttc cgtttctgcg ccgccagatt ggcatgattt
    32041 tccaggatca ccatctactg atggaccgta ctgtctacga taacgtggcg atcccgctga
    32101 ttatcgccgg tgccagcggt gacgatattc gtcgccgggt gtcggcggca ctggataaag
    32161 tcgggctact ggacaaagcg aagaacttcc ctattcagct ttctggcggt gaacaacagc
    32221 gtgttggcat tgcccgcgcg gtggtgaata agcccgcggt actgctggcg gacgaaccga
    32281 ctggtaacct ggacgacgcg ttgtcggaag ggattctacg tctgtttgaa gagtttaacc
    32341 gcgttggggt aaccgtattg atggcaacgc acgacatcaa cctgatttcg cggcgttcct
    32401 atcgcatgct caccctgagc gatggtcact tgcatggagg cgtgggccat gaataagcgc
    32461 gatgcaatca atcatattcg gcggtttggc gggcgtctgg atcgcttccg taaatcggtc
    32521 ggcggctcag gcgacggcgg tcgtaacgcg ccgaaacgcg cgaaatcctc gccaaaaccg
    32581 gtaaatcgca aaaccaacgt tttcaacgaa caggtgcgct atgccttcca cggcgcattg
    32641 caggatctga aaagcaaacc gttcgccacg tttttaacgg tgatggttat cgccatctct
    32701 ctgacgctgc ccagcgtctg ttatatggtg tacaaaaacg ttaaccaggc ggcgacgcaa
    32761 tattatccgt caccgcaaat cactgtttat ctgcaaaaaa cgctggacga tgacgctgct
    32821 gcgggcgtgg tggcacagtt gcaggccgag cagggcgtgg agaaagtgaa ttatctttct
    32881 cgtgaagacg cactgggcga gttccgtaac tggtctggtt ttggtggtgc actggatatg
    32941 ctggaagaaa acccgcttcc ggcagtggcg gtggtgatcc cgaaactcga ttttcagggg
    33001 acggaatcac tcaatacgct gcgtgatcgt atcacgcaga ttaacggcat tgacgaagtg
    33061 cggatggatg acagctggtt tgcccgtctg gcggcgttga ccgggctggt cgggcgcgtt
    33121 tcggcgatga tcggcgtgtt gatggtggcg gcagtgttcc tcgtcatcgg taacagtgta
    33181 cgcctgagta tctttgctcg ccgtgactcc atcaacgtgc agaaactgat tggtgcgacg
    33241 gatggtttca tcctgcgtcc gttcctgtac ggtggcgcac tgctgggatt ttctggcgca
    33301 ttgttgtcat taattttgtc agaaattctg gtgctgcgat tgtcatcggc ggttgcggaa
    33361 gtggcacagg ttttcggaac gaagtttgat atcaatggtt tatcgttcga tgaatgcctg
    33421 ctgttgctgc tggtatgctc gatgattggc tgggtggcag cgtggcttgc cacggtacaa
    33481 catttacgcc actttacgcc tgaataataa aagcgtgtta tactctttcc ctgcaatggg
    33541 ttccgtagca gggaaagaga ccccgttgtc tcttcccggt atttcatctc tatgtcacat
    33601 tttgtgcgta atttattcac aagcttgcat tgaacttgtg gataaaatca cggtctgata
    33661 aaacagtgaa tgataacctc gttgctctta agctctggca cagttgttgc taccactgaa
    33721 gcgccagaag atatcgattg agaggatttg aatgactgac aaaatgcaaa gtttagcttt
    33781 agccccagtt ggcaacctgg attcctacat ccgggcagct aacgcgtggc cgatgttgtc
    33841 ggctgacgag gagcgggcgc tggctgaaaa gctgcattac catggcgatc tggaagcagc
    33901 taaaacgctg atcctgtctc acctgcggtt tgttgttcat attgctcgta attatgcggg
    33961 ctatggcctg ccacaggcgg atttgattca ggaaggtaac atcggcctga tgaaagcagt
    34021 gcgccgtttt aacccggaag tgggtgtgcg cctggtctcc ttcgccgttc actggatcaa
    34081 agcagagatc cacgaatacg ttctgcgtaa ctggcgtatc gtcaaagttg cgaccaccaa
    34141 agcgcagcgc aaactgttct tcaacctgcg taaaaccaag cagcgtttgg gctggtttaa
    34201 ccaggatgaa gtcgaaatgg tggcccgtga actgggcgta accagcaaag acgtacgtga
    34261 gatggaatca cgtatggcgg cacaggacat gacctttgac ctgtcttccg acgacgattc
    34321 cgacagccaa ccgatggcac cggtgctcta tctgcaggat aaatcatcta actttgccga
    34381 cggcatcgaa gatgataact gggaagagca ggcggcaaac cgtctgaccg acgcgatgca
    34441 gggtctggac gaacgcagcc aggacatcat ccgtgcgcgc tggctggacg aagacaacaa
    34501 gtccacgttg caggaactgg ctgaccgtta cggtgtttcc gctgaacgtg tacgccagct
    34561 ggaaaagaac gcgatgaaaa aactgcgcgc tgctatagaa gcgtaatttc cgctattaag
    34621 cagagaaccc tggatgcgag tccggggttt ttgttttttg ggcctcaaca ataatcaatt
    34681 tcccctccga caaaacgcca atccccacgc agattgttaa taaactgtca aaatagctat
    34741 tccaatatca taaaaatcgg gtatgtttta gcagagtatg ctgctaaagc acgggtagtc
    34801 atgcataaaa cgaaataaag tgctgaacaa caacatcaca acacacgtaa taaccagaag
    34861 aatggggatt ctcaggatga acataaaggg taaagcgtta ctggcaggat gtatcgcgct
    34921 ggcattcagc aatatggctc tggcagaaga tattaaagtc gcggtcgtgg gcgcaatgtc
    34981 cggtccggtt gcgcagtacg gtgaccagga gtttaccggc gcagagcagg cggttgcgga
    35041 tatcaacgct aaaggcggca ttaaaggcaa caaactgcaa atcgtaaaat atgacgatgc
    35101 ctgtgacccg aaacaggcgg ttgcggtggc gaacaaagtc gttaacgacg gcattaaata
    35161 tgtgattggt cacctctgtt cttcatcaac gcagcctgcg tctgacatct atgaagacga
    35221 aggcatttta atgatcaccc cagcggcaac cgcgccggag ctgaccgccc gtggctatca
    35281 gctgatcctg cgcaccaccg gcctggactc cgaccagggg ccgacggcgg cgaaatatat
    35341 tcttgagaaa gtgaaaccgc agcgtattgc tatcgttcac gacaaacagc aatacggcga
    35401 aggtctggcg cgtgcggtgc aggacggcct gaagaaaggc aatgctaacg tggtgttctt
    35461 tgatggtatc accgccgggg aaaaagattt ctcaacgctg gtagcgcgtc tgaagaaaga
    35521 gaatatcgac ttcgtttact acggcggtta tcacccggaa atggggcaaa tcctgcgtca
    35581 ggcacgcgcg gcagggttga aaactcagtt tatggggccg gaaggcgtgg ctaacgtctc
    35641 gctgtctaac attgcgggcg aatcagcgga agggctgctg gtgaccaagc cgaagaacta
    35701 cgatcaggtt ccggcgaaca aacccattgt tgatgcgatc aaagcgaaaa aacaggaccc
    35761 aagcggcgca ttcgtttgga ccacctacgc cgcgctgcaa tctttgcagg cgggcctgaa
    35821 tcagtctgac gatccggctg agatcgccaa atacctgaaa gcgaactcgg ttgataccgt
    35881 gatgggtccg ctgacgtggg atgagaaagg tgatctgaaa ggctttgagt tcggtgtgtt
    35941 tgactggcac gccaacggca cagcgaccga tgcgaagtaa ttgttaatcg gcaactttgg
    36001 gttgccgcca aattgctaat atagagtacg ttgcttcatg ccggatgcgg cgtaaacgcc
    36061 ttatccggcc tacaagatcc aaagaaatca gtaaattgca acacacattg taggcctgat
    36121 aagcgtagcg catcaggcaa tttctcgttg gtcatcactt ttgaaatcaa acttgacgat
    36181 taacgcttct cccagccgcc ctgctgtgcc gtaaatccca gtgcctgcat aaacgccgtc
    36241 atcacaccgc gatcttccac gcctgcatcc gccatccacc agcatgaaac gccaggattg
    36301 ttacgcaaaa cctcttccag cagatattgc cccacaccgc gacgtcgagt gacttcccgc
    36361 acgcgcaggg aatccagtgc cccctcggtg ccgcttaagg ttacccgcac ggccgcaagc
    36421 aggcgctcgt taaaacgcgc ggcgtagatg cggtggttat cgtcaacctg taaagaggaa
    36481 ggggaatact ccggccagat cttttgcagg tcaatccggt cctggtcgct aaatttttct
    36541 aatcgaatga tggtcagctt catgggtaac ccgtgtaaat cacaaaagtg gaaccagtgt
    36601 agcgaaataa tttaatcgga ggctttctct tttttatttc ttttggcagg tgattaattt
    36661 tttaacagca ataattacaa agttaaaaca ttagagaatg aaaaatgtcc agcataatcc
    36721 cctgaatgat agtgaattat tccgcccttt gtgccgttat tttatgctga caaaggcact
    36781 tttttctgtt tatctatcaa taaattcaga atattatctg ttcttaatcg actgaaaaat
    36841 agggatttta atcgctatca tcacaaaata ctgcgctaac cccttaatca gacaggcaaa
    36901 aacagtgcag tataaaaaaa gaacagtctg atttgttaac acataaaaac aaagcaacac
    36961 aacatcacga atggggattt ttgactatga aacggaatgc gaaaactatc atcgcaggga
    37021 tgattgcact ggcaatttca cacaccgcta tggctgacga tattaaagtc gccgttgtcg
    37081 gcgcgatgtc cggcccgatt gcccagtggg gcgatatgga atttaacggc gcgcgtcagg
    37141 cgattaaaga cattaatgcc aaagggggga ttaaaggcga taagctggtt ggcgtggaat
    37201 atgacgacgc ctgcgacccg aaacaagccg ttgcggtcgc caacaaaatc gttaacgacg
    37261 gcattaaata cgttattggt catctgtgtt cttcttctac tcaacctgca tcagatatct
    37321 atgaagacga aggtattctg atgatctcgc cgggagcgac caacccggag ctgacccaac
    37381 gcggttatca acacattatg cgtactgccg ggctggactc ttcccagggg ccaacggcgg
    37441 caaaatacat tcttgagacg gtgaagcccc agcgcatcgc catcattcac gacaaacaac
    37501 agtatggcga agggctggcg cgttcggtgc aggacgggct gaaagcggct aacgccaacg
    37561 tcgtcttctt cgacggcatt accgccggcg agaaagattt ctccgcgctg atcgcacgcc
    37621 tgaaaaaaga aaacatcgac ttcgtttact acggcggtta ctacccggaa atggggcaga
    37681 tgctgcgcca ggcccgttcc gttggcctga aaactcagtt tatggggccg gaaggtgtgg
    37741 gtaacgcatc attgtcgaat attgccggtg atgctgccga aggcatgttg gtcactatgc
    37801 caaaacgcta tgaccaggat ccggcaaacc agggcatcgt tgatgcgctg aaagcagaca
    37861 agaaagatcc gtccgggcct tatgtctgga tcacctacgc ggcggtgcaa tctctggcaa
    37921 ctgcccttga gcgtaccggc agcgatgagc cgctggcgct ggtgaaagat ttaaaagcta
    37981 acggtgcaaa caccgtgatt gggccgctga actgggatga aaaaggcgat cttaagggat
    38041 ttgattttgg tgtcttccag tggcacgccg atggttcatc cacggcagcc aagtgatcat
    38101 cccaccgccc gtgaaatgcg ggcgggttta gaaaggttac cttatgtctg agcagttttt
    38161 gtatttcttg cagcagatgt ttaacggcgt cacgctgggc agtacctacg cgctgatagc
    38221 catcggctac accatggttt acggcattat cggcatgatc aacttcgccc acggcgaggt
    38281 ttacatgatc ggcagctacg tctcatttat gatcatcgcc gcactgatga tgatgggcat
    38341 tgataccggc tggctgctgg tggcggcggg attcgtcggc gcaatcgtca ttgccagcgc
    38401 ctacggctgg agtatcgaac gggtggccta tcgcccggtg cgtaactcca agcgcctgat
    38461 tgcactcatc tcggcaatcg gtatgtccat cttcctgcaa aactacgtca gcctgaccga
    38521 aggttcgcgc gacgtggcgc tgccgagcct gtttaacggt cagtgggtgg tggggcatag
    38581 cgaaaacttc tctgcctcta ttaccaccat gcaggcggtg atctggattg tcaccttcct
    38641 cgccatgctg gcgctgacga ttttcattcg ttattcccgt atggggcgcg cctgccgtgc
    38701 ctgcgccgaa gatctgaaaa tggcgagtct gcttggcatt aacaccgacc gggtgattgc
    38761 gctgaccttt gtgattggcg cggcgatggc ggcggtggcg ggtgtgctgc tcggtcagtt
    38821 ctacggcgtc attaacccct atatcggctt tatggccggg atgaaagcct ttaccgctgc
    38881 ggtgctcggt gggattggca gcattccggg agcgatgatt ggcgggctga ttctggggat
    38941 tgcggaggcg ctctcttctg cctatctgag tacggaatat aaagatgtgg tctcattcgc
    39001 cctgctgatt ctggtgctgc tggtgatgcc gaccggtatt ctgggtcgcc cggaggtaga
    39061 gaaagtatga aaccgatgca tattgcaatg gcgttgctct ctgccgcgat gttttttgtc
    39121 ctggcgggcg tctttatggg cgtacaactg gagctggatg gcaccaaact ggtggtcgac
    39181 acggcttcag acatccgttg gcagtgggtg tttatcggca cggcggtggt ctttttcttc
    39241 cagcttttgc gaccggcttt ccagaaaggg ttgaaaagcg tttccggacc gaagtttatt
    39301 ctgcccgcca ttgatggctc cacggtgaag caaaagctgt ttctcgtggc gctgctggtg
    39361 cttgcggtgg cgtggccgtt tatggtctca cgcgggacgg tggatatcgc caccctgacc
    39421 atgatctaca ttatcctcgg actcgggctg aacgtggttg ttggtctttc tggtctgctg
    39481 gtgctggggt acggcggctt ttacgccatc ggcgcttaca cttttgcgct gctcaatcac
    39541 tattacggtt tgggcttctg gacctgtctg ccgattgccg ggttaatggc ggcggcggcg
    39601 ggcttcctgc tcggcttccc ggtgcttcgc ttgcgcggcg actatctggc gatcgttacc
    39661 ctcggtttcg gcgaaattgt gcgcatattg ctgctcaata acaccgaaat taccggcggc
    39721 ccgaacggaa tcagtcagat cccgaaaccg acactcttcg gactcgagtt cagccgtacc
    39781 gctcgtgaag gcggctggga cacgttcagt aatttctttg gcctgaaata cgatccctcc
    39841 gatcgtgtca tcttcctcta cctggtggcg ttgctgctgg tggtgctaag cctgtttgtc
    39901 attaaccgcc tgctgcggat gccgctgggg cgtgcgtggg aagcgttgcg tgaagatgaa
    39961 atcgcctgcc gttcgctggg cttaagcccg cgtcgtatca agctgaccgc ctttaccatc
    40021 agtgccgcgt ttgccggttt tgccggaacg ctgtttgctg cgcgccaggg ctttgtcagc
    40081 ccggaatcct tcacctttgc cgaatcagcc tttgtactgg cgatagtggt gctcggcggt
    40141 atgggctcgc aatttgcagt gattctggcg gctgttctgc tggtggtatc gcgcgagttg
    40201 atgcgtgatt tcaacgaata cagcatgtta atgctcggtg gtttgatggt gctgatgatg
    40261 atctggcgtc cgcagggctt gctgcccatg acgcgcccgc aactgaagct gaaaaacggc
    40321 gcagcgaaag gagagcaggc atgagtcagc cattattatc tgttaacggc ctgatgatgc
    40381 gcttcggcgg cctgctggcg gtgaacaacg tcaatcttga actgtacccg caggagatcg
    40441 tctcgttaat cggccctaac ggtgccggaa aaaccacggt cttcaactgc ctgaccggtt
    40501 tctacaaacc caccggcggc accattttac tgcgcgatca gcatctggaa ggtttgccgg
    40561 ggcagcaaat cgcccgcatg ggcgtggtgc gtaccttcca gcatgtgcgt ctgttccgtg
    40621 aaatgacggt aattgaaaac ctgctggtgg cgcagcatca gcaactgaaa accgggctgt
    40681 tctcaggcct gttgaaaacg ccgtctttcc gtcgcgccca aagtgaagcg ctcgaccgcg
    40741 ccgccacctg gcttgagcgc attggtttgc tggaacacgc caaccgtcag gcgagtaacc
    40801 tggcctacgg tgaccagcgc cgtctggaga tcgcccgctg catggtgacg cagccggaga
    40861 ttttaatgct cgacgaacct gcggcaggtc ttaacccgaa agaaacgaaa gagctggatg
    40921 agctgattgc cgaactgcgc aatcatcaca acaccactat cttgttgatt gaacacgata
    40981 tgaagctggt gatgggaatt tcggaccgaa tttacgtggt caatcagggg acgccgctgg
    41041 caaacggtac gccggagcaa atccgtaata acccggacgt gatccgtgcc tatttaggtg
    41101 aggcataaga tggaaaaagt catgttgtcc tttgacaaag tcagcgccca ctacggcaaa
    41161 atccaggcgc tgcatgaggt gagcctgcat atcaatcagg gcgagattgt cacgctgatt
    41221 ggcgcgaacg gggcggggaa aaccaccttg ctcggcacgt tatgcggcga tccgcgcgcc
    41281 accagcgggc gaattgtgtt tgatgataaa gacattaccg actggcagac ggcgaaaatc
    41341 atgcgcgaag cggtagcgat tgtcccggaa gggcgtcgcg tcttctcgcg gatgacggtg
    41401 gaagagaacc tggcgatggg cggctttttt gccgaacgtg atcagtttca ggagcgcata
    41461 aagtgggtgt atgagctgtt tccacgtctt catgagcgtc gtgttcagcg ggcaggcacc
    41521 atgtccggcg gtgaacagca gatgctggcg attggtcgcg cgctgatgag caatccgcgt
    41581 ttgttgttgc ttgatgagcc atcgctcggt cttgcgccga ttatcatcca gcaaattttc
    41641 gacaccatcg agcagctgcg cgagcagggg atgactatct tcctcgtcga gcagaacgcc
    41701 aaccaggcgc tgaagctggc ggatcgcggc tacgtgctgg aaaacggcca tgttgtgctc
    41761 tccgataccg gcgatgcgct gctggcgaac gaagcagtga gaagtgcgta tttaggcggg
    41821 taaattatcg aatacccggc ggccacgttc tgccgggctc ttcgtccgta tacagacttg
    41881 tttcaactga ccgggaggat taaaaccaga ccacttattt gccacggaaa ggttccctcc
    41941 catgcattat gccgttgttg tcatgcttga attgctttgc tgtattcacg cttaccgtac
    42001 cggacaagag cggtactgga tttttatcat tttctgtttt ccggtgattg gctgcgtggc
    42061 gtatttcgtc atagtgatgc tgtctgaaac gggcgctgac cgtcacgggc atactttgct
    42121 gatgcgcctg cagaacaaac tcaaccctga gcgccacctg cgcaaattaa ccgaagaact
    42181 ggcgattgcc gagacgaatc aaaaccatta tgcgttggct aatgaactgg cgcgtctggg
    42241 gcgttatcac gaagctgttc cccattatca gcaggcgtta agcgggatat tcgcccatga
    42301 agcggcaatg atgctaagcc tggcgcaggc acagtttgct attcaggagt tcgccgcctg
    42361 tcagcagacg ctggaagatg tgatgcgtta taaccctgat tttcagtcgg cagacgggca
    42421 tttgttattt gccagaacgt tagccgcaca agaaaaatac gctgatgcgg aaagcgagtt
    42481 tgaagtattg atttcttatt accccggccc gcaggcgcgt atttactatg cggaaatgtt
    42541 ggcgaaaatg agtcggttac gtgaagccaa cgagcagtat gttgctgtag ttgatacggc
    42601 taaacgctca agacctcatt atagaaaaca ccaccgggaa tggattaaga ccgccaatga
    42661 acggctgaag cagagtgtgg tgcaatgaaa atcttctttg ccgttagacc agtgtcgcgg
    42721 caatcttttg ttaaaattga aaaatgtagc tctcatcggg cagcaaaact ggacgcagaa
    42781 tttgctgcca tttttgctct tcatggtaag gagttctggc gattagctgg ccgttaatcg
    42841 cctcttttca gtgcattctc gggatatccg tgccccatct cccattttcc ctcacccgta
    42901 ccgtcaccgc cttgtcatct ttctgacacc ttactatctt acaaatgtaa caaaaaagtt
    42961 atttttctgt aattcgagca tgtcatgtta ccccgcgagc ataaaacgcg tgaattcgcg
    43021 cattcggtac aacaagagag ataaacgatg aaaccgttac attatacagc ttcagcactg
    43081 gcgctcggac tggcgttaat ggggaatgca caggcagtga cgaccattcc gttctggcat
    43141 tctatggaag gggaactggg taaagaggtg gattctctgg cccaacgttt taacgccgaa
    43201 aacccggatt acaaaattgt accgacctat aaaggcaact acgaacagaa tttaagcgcg
    43261 gggattgccg catttcgtac cggcaatgct ccggctattt tgcaggttta tgaagttggc
    43321 accgccacca tgatggcgtc gaaagccatt aaaccggtat atgacgtgtt taaagaggcg
    43381 gggattcaat tcgatgagtc gcagtttgtg ccgacggttt caggttacta ttccgacagc
    43441 aaaaccggcc acttactctc ccagccgttc aacagctcga ctcccgttct ctattacaac
    43501 aaagacgcct tcaagaaagc ggggttagac ccggaacaac cgccgaaaac ctggcaggat
    43561 ctggcggact atgccgcaaa actaaaagcc tccggcatga agtgcggcta cgccagcggc
    43621 tggcagggct ggatccaact ggaaaacttt agcgcctgga acggtctgcc gtttgccagc
    43681 aaaaacaacg gctttgacgg cacggatgcg gtgctggagt tcaacaagcc ggagcaggtg
    43741 aaacacatcg ccatgctcga agagatgaac aagaagggcg atttcagcta cgtcgggcgt
    43801 aaggatgaat ccaccgagaa gttctataac ggtgattgcg cgatgaccac cgcctcttcc
    43861 ggttctctcg ctaatattcg tgagtacgcc aaatttaact atggcgtagg catgatgcct
    43921 tacgacgccg atgcgaaaga tgcaccgcaa aacgccatta tcggcggggc cagcctgtgg
    43981 gtgatgcagg gcaaagataa agaaacgtat accggcgtgg cgaagttcct cgacttcctc
    44041 gctaagccag aaaatgctgc cgagtggcat cagaaaactg gttatctgcc aatcaccaaa
    44101 gcggcgtatg acttgactcg tgagcagggc ttttatgaga aaaacccagg ggcggatacc
    44161 gcgacgcgtc agatgctgaa taagccgccg ttgccgttca ccaaagggct gcgtctgggc
    44221 aacatgccgc agatccgcgt gattgtggat gaagagctcg agagcgtgtg gaccggcaag
    44281 aagacgccac agcaggcgct ggataccgcc gttgagcgtg ggaatcagtt gctgcgccgt
    44341 tttgagaaat ctacgaagtc ttaatcagtg taatgtcgga tgcgtttcgc ttatctgacc
    44401 tggcatcgcg tgtaagccgg ataagcgaag cgcatccggc acagttcagg aattaaccgt
    44461 aatgtcatca tcccgtccgg tgttccgctc gcgctggctg ccttatctgc tggtcgcgcc
    44521 gcagctcatc atcaccgtta tcttttttat ctggcctgcg ggcgaagcgt tgtggtactc
    44581 gctgcaaagc gtcgatccgt ttggtttctc cagccagttt gtcggcctgg ataacttcgt
    44641 cgcgctgttt catgacagct actatatcga ctccttctgg acgacgataa aattcagcac
    44701 ctttgtcacc gtcagcggtt tgctggtatc gctgttcttt gcggcgctgg tggagtacat
    44761 cgtgcgcggc agtcgtttct atcaaacctt gatgctgctg ccgtatgccg tggctcccgc
    44821 cgttgccgcc gtattgtgga tatttttgtt taaccccggt cgcgggctga tcactcattt
    44881 tctcgccgag ttcggctacg actggaacca cgcgcaaaac agcggtcagg cgatgtttct
    44941 ggtggtgttt gcctcggtgt ggaagcaaat cagctacaac tttctgttct tctatgccgc
    45001 gctgcaatcc attccccgtt cgttgattga agccgcagcc attgatggcg caggcccgat
    45061 tcgccgcttc tttaagattg cgctaccgct tatcgctccg gtgagtttct tcctgctggt
    45121 agtgaacctg gtgtatgcct tcttcgacac cttcccggtg atcgacgccg ccacgtccgg
    45181 tgggccggtg caggccacca cgacgctgat ttataagatc taccgtgaag gctttaccgg
    45241 actggatctg gcttcgtctg ccgcacagtc ggtggtgttg atgttcctcg tcatcgtgct
    45301 gacggtggtg cagttccgct atgttgaaag caaggtgcgt taccaatgat tgagaaccgt
    45361 ccgtggctga cgatattcag ccataccatg ctgatcctcg ggatcgcggt gatcctattc
    45421 ccgctgtacg tggcgtttgt cgcggcgacg ctggataaac aggccgtcta cgccgcgccg
    45481 atgacgctca tccccggcac gcatctgctg gaaaacatcc acaacatctg ggtaaacggg
    45541 gtaggcacaa atagtgcgcc gttctggcgg atgttgctta acagcttcgt gatggcgttc
    45601 agcattacgc tcggcaaaat taccgtctcg atgctctcgg catttgccat tgtctggttt
    45661 cgttttccgc tacgtaacct cttcttctgg atgattttta tcaccctgat gctgccggtt
    45721 gaagtgcgta tcttcccgac ggtggaagtc atcgccaacc tgaaaatgct cgacagctac
    45781 gccggtttaa cgctgccgct gatggcctcg gcgaccgcta ccttcctgtt ccgccagttc
    45841 tttatgacgc tgccggatga gctggtggag gccgcgcgga tcgacggcgc gtcaccaatg
    45901 cgtttctttt gcgacatcgt ttttccgttg tccaaaacta atctggcggc gctgtttgtg
    45961 atcaccttta tttacggctg gaaccagtat ttgtggccgt tgttgattat taccgacgtg
    46021 gatctcggca ccaccgtggc agggatcaaa gggatgatcg ccactggcga aggcaccaca
    46081 gaatggaact cagtgatggc ggcgatgttg ttaaccctga tcccaccagt agtgattgtt
    46141 ttagtgatgc agcgtgcctt cgtgcgcggc ctggtcgata gtgagaaata agatggcagg
    46201 actgaaatta caggcagtaa ccaaaagctg ggatggcaaa acccaggtga ttaaaccgct
    46261 gacccttgat gtggcggatg gcgaatttat cgtgatggtc gggccgtcag gctgcgggaa
    46321 atcgacgctg ctgcgcatgg ttgccgggct ggagcgggtg acggaaggcg atatctggat
    46381 caacgaccag cgggtgaccg aaatggagcc gaaagatcgc gggattgcga tggtgttcca
    46441 gaactacgcg ctttatccgc atatgagtgt tgaagaaaac atggcgtggg ggctgaaaat
    46501 tcgcggcatg ggcaagcagc aaattgccga gcgcgttaaa gaggcggcgc gcattctgga
    46561 actggacggt ctgcttaagc gccgcccgcg cgagctttcc ggcggtcagc gtcagcgtgt
    46621 ggcgatgggc cgcgcgattg tgcgcgatcc ggcggtgttc ctgtttgatg agccactctc
    46681 taacctcgat gccaagctgc gcgtacagat gcgtcttgaa ctgcaacagc tgcaccgtcg
    46741 cctgaaaacg acatcactct acgttactca cgatcaggtt gaggcgatga ccctcgccca
    46801 gcgagtaatg gtgatgaacg gcggcgttgc cgaacagatt ggcacaccag tagaagtcta
    46861 cgaaaagccc gccagcctgt ttgtggcgag ttttattggc agcccggcga tgaatctgct
    46921 gacaggccgc gtgaataacg aaggcacgca tttcgaactg gacggcggta ttgtgctacc
    46981 gctaaacggt ggctaccgtc agtatgccgg gcgtaaaatg actctcggca ttcgcccgga
    47041 acatattgcg ctaagctcgc aagcagaagg tggcgtaccg ctggtgatgg acacgctgga
    47101 gatcctcggc gcagataacc tggcgcacgg acgatggggc gaacagaagc tggtggtgcg
    47161 actggcgcat caggagcgcc cgacggcagg cagcacgctg tggctgcatc tggcggaaaa
    47221 tcagctgcat ctttttgatg gtgaaacagg acaacgagta tgagtaactg gccttatccc
    47281 cgcatcgtcg cacatcgtgg cggcggtaag ttggccccgg aaaacaccct ggcggcaatc
    47341 gacgtcgggg caaaatacgg tcataagatg atcgaatttg acgcgaagtt gtcgaaagat
    47401 ggcgaaatct tcctgctcca tgacgacaat ctcgaacgta ccagcaacgg ctggggcgtc
    47461 gcgggtgaac tgaactggca ggatttacta cgtgtggatg caggcagttg gtacggcaaa
    47521 gcgtttaaag gtgagccgct gccgttgctt tcgcaggtgg cggaacgctg tcgcgaacat
    47581 gggatgatgg cgaatatcga aatcaaaccc accactggca ccgggccatt aacgggcaaa
    47641 atggtggcgc tggcggcacg cgaactgtgg gccggtatga cgccgccgct actgtcatcg
    47701 tttgagattg atgctttaga agccgcacaa caggcagtac cagaactgcc gcgcggtttg
    47761 ttgctggatg agtggcgcga cgactggcga gaactgaccg cgcggctggg ctgcgtctct
    47821 attcatctca attataaatt gcttgataaa gcacgagtga tgcagttgaa agacgccgga
    47881 ttacggattc tggtttatac cgtcaataaa ccccagcgcg cggcagagtt gctgcgctgg
    47941 ggcgtggatt gcatctgtac cgatgcgatt gatgtgattg gtccgaactt tacggcccaa
    48001 tagttttcaa cggtatatca ggctgcggcg tgccgggctg attcagcatg tcgccgtttc
    48061 tcctctccgg caacatatgc tgctgattca acgaactatc cggattacgg ttgctgttta
    48121 acatcccgcc attggtgttg ggcagcattt gctgccgggc gggatttcgc tcccccggct
    48181 gtgactgcaa cacccgctga gaattattgt ttatctggtt ttctaaatgc tgttgttgca
    48241 actgagtttg cgttttcagt tgctgattca gcatcccttt ttgctggatt tgctgcgttt
    48301 gcatctgggt ttgcatccgc tgctgactgg ggatctgata ccccggctgg ttagggttgt
    48361 tcagagtatt aatgggctgt gcaaagccga caaacggcag gagtgccgta agaatcagaa
    48421 gtcgtttcat cgcgtatcct cctctgaaga tattctttaa gtttactcct ttcctgcaat
    48481 catcaggatt aattcagagt tatataccag gcttagctgg ggttgcccct taatctctgg
    48541 agaataacga tgataaaacc gacgttttta cgccgggtgg ccattgctgc tctgttcaca
    48601 ggaagttgtt ttagtacggt agccgcgcca cctgcgccgt ctcctgtttc ttacggcgtg
    48661 gaggaagatg tctttcatcc ggtacgcgcg aaacagggga tggtggcatc tgtagatgct
    48721 actgccactc aggtgggggt ggatatcctc aaggagggcg ggaatgccgt tgatgccgcc
    48781 gtggctgtgg gctacgcgct ggcggtaacg catccgcagg cagggaatct gggcggtggt
    48841 ggttttatgc tgatccgctc gaaaaatggc aataccaccg ctatcgattt ccgcgaaatg
    48901 gcacccgcca aagcgacccg cgatatgttc cttgatgacc agggcaaccc ggacagcaaa
    48961 aaatcactca cttcgcatct ggcttccggc acgcccggta cggtcgctgg tttctcgctg
    49021 gcgttggata aatacggcac aatgccgttg aacaaagtgg tgcagccagc gtttaaactg
    49081 gcacgcgatg gttttatcgt taacgacgcg ctggctgacg atctcaaaac ctacgggagc
    49141 gaagtgctgc cgaatcacga aaacagcaag gctatcttct ggaaagaggg cgagccgctg
    49201 aaaaagggcg acaagctggt gcaggcgaac ctggcaaaga gcctggagat gattgccgaa
    49261 aacggcccgg acgaattcta caaaggcacg atagcggaac agattgccca ggagatgcag
    49321 aaaaacggtg gcctgatcac taaagaagat ttagcggcgt ataaagcggt cgaacgcaca
    49381 ccgataagcg gcgattatcg cgggtatcag gtttactcca tgccaccgcc atcctccggc
    49441 gggatccata tcgtacaaat cctcaatatt ctggaaaact tcgatatgaa gaaatacggt
    49501 tttggcagcg ccgacgcgat gcagatcatg gcagaagcgg agaaatatgc ctacgctgac
    49561 cgctcggaat atcttggcga cccggatttt gtcaaagtcc cgtggcaggc gctgaccaat
    49621 aaagcctatg ccaaatctat tgccgagcaa atcgatatca ataaagcgaa accgtccagc
    49681 gagattcgcc ctggcaagct tgcgccttat gagagtaatc aaactaccca ttactcagtg
    49741 gtggataaag acggtaacgc cgtggcggtg acctatacgc tgaacaccac cttcggtacg
    49801 ggtattgtcg cgggcgagag cggtattctg cttaataacc agatggatga tttctccgcc
    49861 aaaccgggcg taccgaacat ttatgggctg gtgggcggtg atgccaacgc cgtcgggccg
    49921 aacaaacgcc cgctgtcatc gatgtcaccc accattgtgg tgaaagacgg taaaacctgg
    49981 ctggtcaccg gtagccctgg cgggagccgg attattacca ccgtgcttca aatggtggtg
    50041 aatagcatcg attatggcat gaacgtcgcc gaagcgacca atgcgccgcg tttccaccat
    50101 cagtggttgc cggacgagct gcgtgtcgaa aaagggttta gcccggatac gctcaagctg
    50161 ctggaagcaa aaggtcagaa agtggcgctg aaagaggcga tgggcagtac acaaagcatt
    50221 atggttgggc cggacggtga gctgtatggt gcatccgacc cgcgctcggt ggatgattta
    50281 actgcggggt attaaggtta gcggccctct tcgtgggaag agggctgttt tcttgaacaa
    50341 gataaattag tgtctggatt gcttttacta ttgctccgta atatataatc ataattatcc
    50401 tg
//
LOCUS       DAZFKF010000028        47743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFKF010000028 DAZFKF010000000
VERSION     DAZFKF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.028"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            249..401
                     /gene="mokC"
                     /locus_tag="ACHKA1_001992"
     CDS             249..401
                     /gene="mokC"
                     /locus_tag="ACHKA1_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO5679648.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(505..1635)
                     /gene="dnaJ"
                     /locus_tag="ACHKA1_001993"
     CDS             complement(505..1635)
                     /gene="dnaJ"
                     /locus_tag="ACHKA1_001993"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO5679649.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1724..3640)
                     /gene="dnaK"
                     /locus_tag="ACHKA1_001994"
     CDS             complement(1724..3640)
                     /gene="dnaK"
                     /locus_tag="ACHKA1_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO5679650.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4017..4421
                     /gene="yaaI"
                     /locus_tag="ACHKA1_001995"
     CDS             4017..4421
                     /gene="yaaI"
                     /locus_tag="ACHKA1_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO5679651.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4447..5160
                     /gene="msyB"
                     /locus_tag="ACHKA1_001996"
     CDS             4447..5160
                     /gene="msyB"
                     /locus_tag="ACHKA1_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO5679652.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5309..5875
                     /gene="satP"
                     /locus_tag="ACHKA1_001997"
     CDS             5309..5875
                     /gene="satP"
                     /locus_tag="ACHKA1_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO5679653.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5910..6497)
                     /gene="mog"
                     /locus_tag="ACHKA1_001998"
     CDS             complement(5910..6497)
                     /gene="mog"
                     /locus_tag="ACHKA1_001998"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO5679654.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6612..7565)
                     /gene="tal"
                     /locus_tag="ACHKA1_001999"
     CDS             complement(6612..7565)
                     /gene="tal"
                     /locus_tag="ACHKA1_001999"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO5679655.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7844..9274
                     /gene="yaaJ"
                     /locus_tag="ACHKA1_002000"
     CDS             7844..9274
                     /gene="yaaJ"
                     /locus_tag="ACHKA1_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO5679656.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGHQLSRDAWDDVSQE"
     gene            9344..10120
                     /gene="yaaA"
                     /locus_tag="ACHKA1_002001"
     CDS             9344..10120
                     /gene="yaaA"
                     /locus_tag="ACHKA1_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO5679657.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10273..10569)
                     /gene="yaaX"
                     /locus_tag="ACHKA1_002002"
     CDS             complement(10273..10569)
                     /gene="yaaX"
                     /locus_tag="ACHKA1_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO5679658.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(10783..12069)
                     /gene="thrC"
                     /locus_tag="ACHKA1_002003"
     CDS             complement(10783..12069)
                     /gene="thrC"
                     /locus_tag="ACHKA1_002003"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO5679659.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12070..13002)
                     /gene="thrB"
                     /locus_tag="ACHKA1_002004"
     CDS             complement(12070..13002)
                     /gene="thrB"
                     /locus_tag="ACHKA1_002004"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO5679660.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVETAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDARLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13004..15466)
                     /gene="thrA"
                     /locus_tag="ACHKA1_002005"
     CDS             complement(13004..15466)
                     /gene="thrA"
                     /locus_tag="ACHKA1_002005"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO5679661.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15492..15611)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15547..15612)
                     /gene="thrL"
                     /locus_tag="ACHKA1_002006"
     CDS             complement(15547..15612)
                     /gene="thrL"
                     /locus_tag="ACHKA1_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO5679662.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15826..16512)
                     /gene="yjtD"
                     /locus_tag="ACHKA1_002007"
     CDS             complement(15826..16512)
                     /gene="yjtD"
                     /locus_tag="ACHKA1_002007"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO5679663.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16912..17052)
                     /gene="yjjY"
                     /locus_tag="ACHKA1_002008"
     CDS             complement(16912..17052)
                     /gene="yjjY"
                     /locus_tag="ACHKA1_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO5679664.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17148..17864
                     /gene="arcA"
                     /locus_tag="ACHKA1_002009"
     CDS             17148..17864
                     /gene="arcA"
                     /locus_tag="ACHKA1_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO5679665.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17924..19276)
                     /gene="creD"
                     /locus_tag="ACHKA1_002010"
     CDS             complement(17924..19276)
                     /gene="creD"
                     /locus_tag="ACHKA1_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO5679666.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLGELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKDVTTDDELRIWK
                     "
     gene            complement(19334..20758)
                     /gene="creC"
                     /locus_tag="ACHKA1_002011"
     CDS             complement(19334..20758)
                     /gene="creC"
                     /locus_tag="ACHKA1_002011"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO5679667.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVDHEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20758..21447)
                     /gene="creB"
                     /locus_tag="ACHKA1_002012"
     CDS             complement(20758..21447)
                     /gene="creB"
                     /locus_tag="ACHKA1_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO5679668.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21460..21933)
                     /gene="creA"
                     /locus_tag="ACHKA1_002013"
     CDS             complement(21460..21933)
                     /gene="creA"
                     /locus_tag="ACHKA1_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO5679669.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22144..23013
                     /gene="robA"
                     /locus_tag="ACHKA1_002014"
     CDS             22144..23013
                     /gene="robA"
                     /locus_tag="ACHKA1_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO5679670.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23010..23657)
                     /gene="gpmB"
                     /locus_tag="ACHKA1_002015"
     CDS             complement(23010..23657)
                     /gene="gpmB"
                     /locus_tag="ACHKA1_002015"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO5679671.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23709..24221
                     /gene="yjjX"
                     /locus_tag="ACHKA1_002016"
     CDS             23709..24221
                     /gene="yjjX"
                     /locus_tag="ACHKA1_002016"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO5679672.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(24368..24694)
                     /gene="trpR"
                     /locus_tag="ACHKA1_002017"
     CDS             complement(24368..24694)
                     /gene="trpR"
                     /locus_tag="ACHKA1_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO5679673.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24784..26721)
                     /gene="sltY"
                     /locus_tag="ACHKA1_002018"
     CDS             complement(24784..26721)
                     /gene="sltY"
                     /locus_tag="ACHKA1_002018"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO5679674.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26932..28599
                     /gene="ettA"
                     /locus_tag="ACHKA1_002019"
     CDS             26932..28599
                     /gene="ettA"
                     /locus_tag="ACHKA1_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO5679675.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28706..28873)
                     /gene="nadS"
                     /locus_tag="ACHKA1_002020"
     CDS             complement(28706..28873)
                     /gene="nadS"
                     /locus_tag="ACHKA1_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO5679676.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(28907..30139)
                     /gene="nadR"
                     /locus_tag="ACHKA1_002021"
     CDS             complement(28907..30139)
                     /gene="nadR"
                     /locus_tag="ACHKA1_002021"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO5679677.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30160..31542)
                     /gene="radA"
                     /locus_tag="ACHKA1_002022"
     CDS             complement(30160..31542)
                     /gene="radA"
                     /locus_tag="ACHKA1_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO5679678.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31591..32559)
                     /gene="serB"
                     /locus_tag="ACHKA1_002023"
     CDS             complement(31591..32559)
                     /gene="serB"
                     /locus_tag="ACHKA1_002023"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO5679679.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32665..33309
                     /gene="ytjB"
                     /locus_tag="ACHKA1_002024"
     CDS             32665..33309
                     /gene="ytjB"
                     /locus_tag="ACHKA1_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO5679680.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33337..34353
                     /gene="lplA"
                     /locus_tag="ACHKA1_002025"
     CDS             33337..34353
                     /gene="lplA"
                     /locus_tag="ACHKA1_002025"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO5679681.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFTHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            34385..34648
                     /locus_tag="ACHKA1_002026"
     CDS             34385..34648
                     /locus_tag="ACHKA1_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5679682.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(34732..35451)
                     /gene="deoD"
                     /locus_tag="ACHKA1_002027"
     CDS             complement(34732..35451)
                     /gene="deoD"
                     /locus_tag="ACHKA1_002027"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO5679683.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35508..36731)
                     /gene="deoB"
                     /locus_tag="ACHKA1_002028"
     CDS             complement(35508..36731)
                     /gene="deoB"
                     /locus_tag="ACHKA1_002028"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO5679684.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(36783..38105)
                     /gene="deoA"
                     /locus_tag="ACHKA1_002029"
     CDS             complement(36783..38105)
                     /gene="deoA"
                     /locus_tag="ACHKA1_002029"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO5679685.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38232..39011)
                     /gene="deoC"
                     /locus_tag="ACHKA1_002030"
     CDS             complement(38232..39011)
                     /gene="deoC"
                     /locus_tag="ACHKA1_002030"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO5679686.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIDIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39269..40819
                     /gene="yjjI"
                     /locus_tag="ACHKA1_002031"
     CDS             39269..40819
                     /gene="yjjI"
                     /locus_tag="ACHKA1_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO5679687.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            40791..41654
                     /gene="yjjW"
                     /locus_tag="ACHKA1_002032"
     CDS             40791..41654
                     /gene="yjjW"
                     /locus_tag="ACHKA1_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO5679688.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVEEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGVGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALRERG
                     VSRLIFPALYL"
     gene            complement(42051..42833)
                     /gene="yjjV"
                     /locus_tag="ACHKA1_002033"
     CDS             complement(42051..42833)
                     /gene="yjjV"
                     /locus_tag="ACHKA1_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5679689.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVLLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEHAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(42830..43903)
                     /gene="yjjU"
                     /locus_tag="ACHKA1_002034"
     CDS             complement(42830..43903)
                     /gene="yjjU"
                     /locus_tag="ACHKA1_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO5679690.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(44025..44186)
                     /gene="ytjA"
                     /locus_tag="ACHKA1_002035"
     CDS             complement(44025..44186)
                     /gene="ytjA"
                     /locus_tag="ACHKA1_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO5679691.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(44313..44918)
                     /gene="osmY"
                     /locus_tag="ACHKA1_002036"
     CDS             complement(44313..44918)
                     /gene="osmY"
                     /locus_tag="ACHKA1_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO5679692.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(45311..46897)
                     /gene="prfC"
                     /locus_tag="ACHKA1_002037"
     CDS             complement(45311..46897)
                     /gene="prfC"
                     /locus_tag="ACHKA1_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO5679693.1"
                     /translation="MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGT
                     VKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTL
                     TAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
                     LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAV
                     GEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAP
                     APMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVR
                     TAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNF
                     APELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVA
                     RLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMV
                     NLRLAQERYPDVQFHQTREH"
     gene            47117..47365
                     /locus_tag="ACHKA1_002038"
     CDS             47117..47365
                     /locus_tag="ACHKA1_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO5679694.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT        11263 a        12954 c        11993 g        11533 t
ORIGIN      
        1 attaaatcat taaaaaatta cgccataaaa atgcgaggcg tcttccggat tagaatcgat
       61 aaaacagaat gggttaaaga gagcgatata atagcggcgg gtgcttgagg ctgtctgtct
      121 caggcattag ctgaacggca gatagagaaa agccccgagt gatattttac catcaacccg
      181 aggcccccta tatgctgaac acatgtagag tgcctcttac tgaccgtaag gtcaaggaga
      241 agagagcaat gaagcagcat aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg
      301 ccgtagtggc ggcgctggta acgagaaaag acctctgtga ggttcacatc cgaactggcc
      361 agacggaggt tgctgttttc acggcttacg aatccgagta agagcaacgg cggggagtga
      421 tccccgccac tttacaggtg ctcgcatatc ttcaacgcac cctattttta cccaggcctg
      481 cccacgggca ggcttttggg gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc
      541 atcaaagaag ctctttgagc gcgggctgtt gtgctcgccg gttgggccac cgaagctttc
      601 ttgcagctct tgcagcagct gtttctgctt ctcgttcagg cctacaggtg tttcgacgac
      661 aacgcggcac agcaaatcac cctgtgcgcc accgcggaca gacttgacgc ctttaccgcg
      721 catacggaat agcttaccgg tctgggtttc gccaggcact ttcagtttga cgcgaccatc
      781 aagggtcggt acttcgattt cgccacccag cgccgccata gcgaagttga tcgggacttc
      841 gcaatacagg ttgttgcctt cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac
      901 gtacagatcg cctgccggtg cgccatgctc gcccgcttca ccttcgcccg caagacggat
      961 gcggtctcca gtgtccaccc ctgccgggat tttaacggac agcgttttgc tgcgctcaac
     1021 acgaccatga ccatgacatt tgttgcacgg atctttgatc agcgtaccgc ggccctgaca
     1081 gtgtggacag gtctgctgca cggcaaagaa accctggcgc atctgcacct gaccagaacc
     1141 atgacaggtc ggacaggtct gcggctgtgt acctggtttt gcaccgctac cgtggcaaac
     1201 gtcacactct tccagagtcg gaatgcggat ctctttggtc acgccacgta cagcttcttc
     1261 gagggtgagc tccatgttat agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg
     1321 tccgccgcca aaaatatcgc cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc
     1381 gccaaaaccg ccgccgccca tgccaccttg ctcaaacgca gcatgaccat actgatcgta
     1441 tgccgcacgt ttttgcgagt cggtcagaac ttcataagct tccttgatct ctttaaattt
     1501 cgcctcggcc tctttgtcac cctggttacg gtccgggtgg tatttcatgg ccaggcgttt
     1561 gtaggccttt ttgatttcac gctcttccgc tgttttggaa acgcctaaaa tctcgtaata
     1621 atcttgctta gccatctttt ttaaattgcc cctagatgaa tgcacgggcg gagaggaaat
     1681 tccccttcgc ccgtgtcagt ataattaccc gtttacaggg cgattatttt ttgtctttga
     1741 cttcttcaaa ctcagcgtcg acaacatcgt catctttcgc gttgtttgca gaagcatcag
     1801 caccggcagt ctgctgctgg gcatgttgct gctgggcgat ttccatcagt ttctgggaaa
     1861 cctgtgccag ctcctgcatt ttcgcttcga tagcggcttt gtcttcacct ttcagagcag
     1921 tttccagtgc agtcagcgca gactcgatag cagttttgtc gtcagccggc agtttgtcgc
     1981 ctgcttcttc aacctgctta cgggtgctgt gcagcagatg gtcgccctgg ttgcgagtct
     2041 gtaccagctc ttcaaactta cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt
     2101 tctggatttc atcttcgttc agaccagaag aagccttgat ggtgatcttc tgctctttac
     2161 cgctgttttt atctttcgcg gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa
     2221 cttcgatctg cggcataccg cgcggtgccg ggttgatacc atccaggttg aactgaccca
     2281 gagatttgtt atcagccgca cgtttacgtt caccctgcag cacgtggatg gttaccgcag
     2341 actggttgtc ttcagcggta gagaacacct ggctgtgctt ggtcgggata gtggtgtttt
     2401 tcgcgatcag cgtcgtcatc acaccgccca tggtttcgat acccagagac agcggggtaa
     2461 cgtccagcag cagtacgtct tttacgtcac cagtcagaac accaccctga acagcagcac
     2521 cgattgctac agcttcgtcc gggttaacgt ctttacgcgg ctctttacca aagaactcag
     2581 caactttctt ctgaaccatt ggcatacgag tctgaccacc aacgaggata acgtcgtcga
     2641 tatcagatac ggacaggcca gcgtcctgca gtgcaacttt cagcggctca atggaacggt
     2701 ttaccagatc ttcaaccagg ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt
     2761 tcggaccggt cgcgtctgca gtgatgtacg gcaggttaac gtcggtctgc tgagcggaag
     2821 acagttcgat tttcgctttt tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt
     2881 tgcgcaggtc aatgccctga tctttcttga attcttcaac cagatagttg atcagacggc
     2941 tgtcgaagtc ttcaccaccc aggtgggtat caccgttggt tgccagaact tcgaaggttt
     3001 tttcgccgtc aacttcgtcg atttcaataa tagaaatatc gaaagtacca ccacccaggt
     3061 cataaaccgc gatagtacgg ttgccagtac ctttgtccag accgtaagcc agcgcagctg
     3121 cagtcggttc gttgatgata cgttttactt cgagaccagc gatacggcct gcgtctttgg
     3181 ttgcctgacg ctgagcatcg ttaaagtatg ccggtacggt gataacagct tcagttaccg
     3241 gttcacccag gtaatcttca gcggttttct tcattttttt cagcacttca gcagaaatct
     3301 gcggcggtgc cattttctgg cctttaactt cgacccatgc gtcgccgtta tcagcagcaa
     3361 taattttgaa cggcatgatg gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc
     3421 caatcaggcg tttaatcgca aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag
     3481 ccggctgacc aaccagagtt tcaccatcct gggtataggc aatgatagaa ggcgtggtgc
     3541 gatcgccttc ggcgttctcc agcacacgag gagtggtgcc atccataatc gctacacaag
     3601 agttggtagt acccaggtcg ataccaatta ttttacccat ctaaacgtct ccactatata
     3661 ttcggtcatc atgtggttgt gagtctgtaa taggggcgaa acgtctggtt tcaactgccc
     3721 aatttcattt ttttgcagac tcactcactg cggttgacta ctaaatgggg tcgtaaccca
     3781 cgtcatcaag ggggagatgc aaaaattttt tgtgcttttg cgcagaaatt tcgcgatatg
     3841 gcaggacaag acgtaaaatt gacagcaatt ttattcatat aaagaatgaa tcgcagtcaa
     3901 ggtattcatc aaaaaattgt aataaaaaga aaagattacg tgcctgaatc ttctctttat
     3961 cagcagtaaa ctagtgggta ttcatccccc tacctcttcc cactaagaga atccttatga
     4021 aatccgtttt tacgatttcc gccagcctgg cgattagcct gatgctgtgc tgcacggcgc
     4081 aggcaaacga ccataaactc ctcggcgtca ttgcaatgcc gcgtaacgaa accaacgatc
     4141 tggcgctgaa acttcctgtt tgtcgcattg tgaaacgcat acaactctct gccgaccatg
     4201 gcgatttaca gttaagcggt gcatcggttt atttcaaagc cgcccgtagc gccagtcaga
     4261 gcctgaatat tccttcagaa ataaaagaag ggcaaaccac tgactggatc aacattaaca
     4321 gcgataacga caataaacgc tgcgtctcaa aaatcacctt ttccggtcat acggtgaact
     4381 catcggatat ggccacgctg aaaattatcg gcgacgacta acgccagaat atagaagcca
     4441 caaaaaatga atgttaatta cctgaatgat tcagatctgg attttctcca gcattgtagt
     4501 gaggaacagt tagcaaattt cgcccggctg ctcacccata atgaaaaagg caaaactcgc
     4561 ctctccagcg tactgatgcg caacgaactg tttaaatcga tggaaggaca tcccgagcaa
     4621 catcgccgca actggcagct gattgccgga gaattacagc attttggtgg cgatagtatc
     4681 gccaacaaac tgcgcggaca cggtaaattg tatcgggcca ttttgctcga tgtttcaaag
     4741 cgattgaagc tgaaagccga caaagagatg tctacgtttg aaattgagca acagttactg
     4801 gaacaatttc tgcgtaatac ctggaagaaa atggacgagg aacataagca ggagtttctg
     4861 cacgcggtcg atgccagggt gaatgagctg gaagagctgc tgccgctgct gatgaaagac
     4921 aaattattgg caaaaggtgt gtcgcatttg ctttccagcc aattgacccg cattttacgc
     4981 acccacgcag caatgagcgt gcttgggcat ggtttgctgc gcggcgcggg tctgggaggc
     5041 cctgtgggcg cagcattaaa tggggttaaa gcggtcagcg gcagcgccta tcgcgtgacg
     5101 attccagccg tactgcaaat cgcctgcctg cgccggatgg ttagcgccac tcaggtctga
     5161 taccggaaac cggggaaaat tttcattttc ccctcaaaag atcgtagaca ctgccccact
     5221 ggctgattat tatgccgcgc cctgaaaaca ctacagttat tcagggaaat tatttcacca
     5281 ttcattcgat gatgattttt gaggaattat gggcaacact aagttggcta atccggcacc
     5341 gctgggcctg atgggcttcg gcatgaccac cattctgctt aacctgcaca acgtgggtta
     5401 tttcgctctg gacggtatta ttcttgccat gggcattttc tacggcggca tcgcgcaaat
     5461 ttttgccggt ctgctggagt acaaaaaagg caacactttc ggtttaaccg cattcacctc
     5521 ttacggttct ttctggctga cgctggttgc gattctgctg atgccgaaac tgggtctgac
     5581 cgatgcgcca aatgcacagt tccttggtgt ctacctgggt ctgtggggcg tatttacgct
     5641 gtttatgttc ttcggcacgc tgaaaggcgc acgcgtcctg caattcgttt tctttagcct
     5701 gaccgtgctg tttgccctgc tggcgatcgg taacattgcc ggtaatgccg cgatcatcca
     5761 ctttgccggc tggattggtc tgatctgcgg tgccagcgca atctatctgg cgatgggtga
     5821 agtgctgaac gagcagtttg gtcgcaccgt tctgccgatt ggtgaatccc actaattgtt
     5881 ttgagatccc cccgctcggg gggatttttt tattcgctaa cgtcgcgtct tgcactcttc
     5941 ggtctgaatg cagcaaccac ttctggtgcc gtttcaacgt atggcccttc cagcaactga
     6001 atgcagtacg gtacgctggc aaaaataccg tgtaccacaa cgttaccctc agcgtccttc
     6061 acaccttcca gcgtctcttt aatagacttc ggctgaccgg gtaagttaag gatcagcgcc
     6121 tgtttgcgaa tcacacccac ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctg
     6181 atctggcgca tctgttcacc aaagccaggc atctcgcggt ccgctactgc cagcgtcgca
     6241 tcgggcgtta cgtcacgacg cgccgggcca gttccgcccg tggtgagcac cagatggcaa
     6301 ctcatttcat ccaccagctc acacaacgtt tgctcgatga tcgcctgctc atcggggatt
     6361 aagcgggttt ccagttcaaa cggcgtggtt agcgccgatg tcagccattc ttccagcgca
     6421 gggatgcctt tatcctgata aacgccactg gatgcgcgat cagagatgga aactaagcca
     6481 atacgtaaag tattcatcaa gatttttccg tttaaacagt ctcgttaaac agaatgatac
     6541 actgcgaagg gagtgacaga caggcttcag aagaggtagc gtgaccgact tcccggtcac
     6601 gctaagaatg attacagcag atcgccgatc attttttcca gtttttcctg gtcaatagca
     6661 aacttacgga taccttccgc cagtttatct actgccattg gatcctggtt gtgctgccac
     6721 aggaactcgg actcagtgat acgcgccgga cgcgctttca cttcgccggt gtaagacagt
     6781 ttacgttcga tagccccttc gctctcagcc agctctttca gcagtgccgg tgcgatggtc
     6841 agacggtcgc agcctgccag ttccagaatt tcgccgatgt tacggaagct tgcgcccata
     6901 accacggttt cataaccgtg ctctttgtag tactggtaga tttcagatac agaaaccacg
     6961 cccggatctt ctgccggagc gtactctttc ttatcggtat tcgctttgta ccagtcaaga
     7021 atacggccaa caaatggaga gatcagaaac acgcccgctt ccgcacaagc acgagcctga
     7081 gcgaaggaga acagcagggt caggttacag ttgatacctt ctttttccag ctgttccgca
     7141 gcacggatac cctgccaggt agaagccagt ttgatcagaa tacgatcgtt gctaatacct
     7201 gcatcgttgt agagtttgat caggcgtttt gctttcgcaa ttgacgcttc ggtgtcatag
     7261 gaaagacgcg catcaacttc agttgagata cggcccggaa ccagtttcag gatttccaga
     7321 ccaatattta ctgccagttt gtcagtcgcg tccacgatct gctgcgcgcg atcgttgctc
     7381 tgctgtttcg cccaggcgac agcatcatca atcaacttac gatattccgg aatctgcgct
     7441 gcgttaagaa tgagagaagg gttggttgtg gcatcctgcg gttgatacag cttcattgcc
     7501 gcgatgtccc cagtgtcggc cactacggtg gtgtactgac gaagggaggt caatttgtcc
     7561 gtcatgatag tatttctctt taaacagctt gttaggggga tgtaaccggt ctgccctgat
     7621 gatatcacga cgcaacgtca gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg
     7681 tttcggattt ctgacactgc gttgatatcg cccgccattt ttaaacaaaa cctcatgtat
     7741 gctacgcaga agttatcaag tacctcgtag cgtatatact tcttaaacaa ttggtaacgt
     7801 ttacacagga aagtcatcgc gaccggcaat aagagggata tgcatgccag attttttctc
     7861 ctttattaac agcgtccttt ggggatcggt aatgatttac ctgctcttcg gcgcaggttg
     7921 ttggttcact tttcgcaccg gatttgtgca gtttcgctac atccgccagt ttggcaaaag
     7981 tcttaaaaat agcattcatc cacagccagg cggtttaacc tcatttcagt cattgtgtac
     8041 cagtcttgcg gcgcgcgtgg gtagcggcaa cctggccggc gttgcgctgg ctattaccgc
     8101 cggtggacct ggagccgtct tttggatgtg ggttgccgcg tttatcggca tggcgacctc
     8161 gtttgccgaa tgttccctgg cacaacttta taaagaacgt gacgtcaatg ggcagtttcg
     8221 tggcggaccg gcatggtata tggcgcgcgg gctggggatg cgctggatgg gcgttctgtt
     8281 cgccgtcttt ttgctcatcg cctacggcat aattttcagc ggagttcagg caaacgctgt
     8341 tgcccgagcc ctgagttttt cttttgattt tcccccgctg gtgacaggca ttattctcgc
     8401 tgtctttgct ctgctggcaa tcactcgcgg tcttcatggc gtcgcccggc tcatgcaggg
     8461 gtttgtcccg ttgatggcga taatctgggt actgaccagc ctggtgattt gcgtaataaa
     8521 tatcgggcaa cttccccacg tcatttggtc tatttttgag agtgcttttg gctggcagga
     8581 agcggcaggc ggcgcggcgg gatatacctt aagccaggcg attactaacg gttttcagcg
     8641 cagtatgttt tccaatgagg cgggaatggg ttcgacgcca aacgcggcag cggcagcggc
     8701 gtcctggcct ccgcatccgg cagcgcaagg aattgtccag atgattggca tttttatcga
     8761 caccctggtc atctgtacgg caagcgccat gctgatatta ctggcgggta acggcacaac
     8821 ctacatgccg ctggaaggta ttcagcttat ccagaaggcg atgcgggtgc taatgggttc
     8881 ttggggtgct gagtttgtta ccctcgtggt tattctgttt gccttcagct ccatcgttgc
     8941 caactacatt tatgccgaaa acaatctctt ctttttacgc ctgaacaacc ctaaagcgat
     9001 ctggtgtttg cggatctgca ccttcgcaac ggtcatcggc ggcaccttgc taagtcttcc
     9061 gctgatgtgg caactggcag atatcataat ggcctgcatg gctattacca atttgaccgc
     9121 cattttactg ctctcgcctg tggttcatac cattgccagt gattatctac gccagcgtaa
     9181 actcggcgtg cgcccggtgt ttgatccgtt gcgttatccg gatatcggtc accagctttc
     9241 tcgggacgcg tgggatgatg tttcgcagga gtaatcacaa ctatcgatca actcattctc
     9301 gttttttgct aaagtcggca taaatttcct gcaaggactg gatatgctga ttcttatttc
     9361 acctgcgaaa acgcttgatt accaaagccc gttgaccacc acgcgatata cactgccgga
     9421 gctgttagac aattcccagc agttgatcca tgaagcgcgg aaactgacgc ctccgcagat
     9481 tagcacgctg atgcgtatca gcgacaaact ggcaggtatc aacgccgctc gctttcatga
     9541 ctggcagcca gatttcacgc cggagaatgc ccgccaggcg attctggcgt ttaaaggtga
     9601 tgtctacacc ggcttgcagg ccgaaacctt cagcgaagac gatttcgatt ttgcccaaca
     9661 gcatttgcga atgctttccg gcttgtatgg cgtactgcgc ccgctcgatt taatgcagcc
     9721 ttatcgtctg gaaatgggga tccgtcttga gaatgcccga gggaaagatc tgtatcaatt
     9781 ctggggagat atcatcacca acaagctgaa cgaggcgctc gcagcacaag gcgataatgt
     9841 ggtgattaat ctggcgtcag atgagtattt taaatcggtg aagccgaaga aattgaatgc
     9901 cgagattatc aagccggtgt tcctcgatga gaagaacggc aaatttaaga taatcagctt
     9961 ctacgctaag aaagcacgcg gtctgatgag tcgtttcatt attgaaaatc ggctgaccaa
    10021 accagagcaa ctgactggtt ttaatagcga aggttacttc tttgatgaag cttcctccag
    10081 caatggcgaa ctggtgttta aacgctacga gcagcgttaa gtttgtagtc aatgccggat
    10141 gcggcgtgga cgccttatcc ggcctacaaa agcatgcaga ttcaatatat tgcagaattc
    10201 acgtaggcct gataagacgc gccagcgtcg catcaggcgc taaatgcagg attgttaccc
    10261 ggcatttgtc atttagcggt gatgtttgcc tggaccatga ccgccgtgat gatcatgatg
    10321 agctttctta tggtgacgcg gcggtggcgg cggtccgtgt gggtgccagc gattgcctcg
    10381 ccattcataa tgttgtttcc accagccgtg gtcgcgccag tgaccgccat cccagtaata
    10441 accgcgatta tcacgatcgc ctatctgtaa ttttactgac gggactaacg taatttccgc
    10501 agcctgcgct gccatgggag cgaccagaac cagggaaagt gcgagtacaa tagattgcat
    10561 ctttttcaca ggttactcct tttatcattg cccgttatgg gtcgatatcc ggagattact
    10621 cgatgagaac ggcggttgtt attctccgca atcctaatct ctaagttacc gcatttattg
    10681 agaatttctc ctttttccct gtcgtttttc tccataattt cttcataaaa aagccgggct
    10741 gcataaaagc aaacccggcc tgattgagat aatgaataga tattactgat gattcatcat
    10801 caatttacgc aacgcagcaa aatcggcggg cagattatgc gaaagcaagg gtaaatcggc
    10861 acgttctgcc agctcttttg gcagatccaa cgtttcaccg agaatcgctt ccacgctctc
    10921 tttaaatttc gccggatgcg cggtgccgag gaacaagcca tattcgcctg gattcaactg
    10981 gtcacgcagc gcacgatacg ctacggcagc gtgcggctcc gaagtgtagc ccagttcttt
    11041 taactcacgc attgtctgtt gcgtggtttc atcatccacg gctgcataac ccagctcttt
    11101 cagttgccag attttgcggc ggaacaactc ttccacacgc ggccagttgt tcggctgact
    11161 cacgtccatc gcgttggata acgtcgcctg agtcgctttg ggtgaccact gaccgtcgtg
    11221 caggaaacgt ggcacggtat cgttcacgtt ggtcgcagca ataaaacgtt tcaccggcag
    11281 accgagtgac ttcgccagca gacccgccgt caaatcgccg aagtttccgc ttggcaccga
    11341 gacaaccagc tggttgcgcg cttcctgcgg cagctgcgca actgcttcaa agtagtagca
    11401 aatctgcgcc agcaaccggc tgatgttaat cgagttagcc gagtttaacc ccagcgccac
    11461 tttcagttct tcatcatcaa acgcctgctt caccagcgcc tgacaggcat cgaaatcgcc
    11521 gtcgatggca acagtttcga tattgccgcc caatgtacag aacagttttt cttgcagtgg
    11581 actgattttg cctcgtggat agaggataac cactttcaca ttcggtaaac cgtagaaagc
    11641 atgagccact gccgctccgg tatcaccgga ggtcgcggtc agaatggtca ctggcttatc
    11701 gcccgcaata tgggtcagca tttgtgccat aaagcgaccg ccgaaatctt taaatgccag
    11761 cgttggcccg tggaacaatt ccagacaacc gacatcgctt tcaacattgg cgaccggagc
    11821 cgggaaggca aacgccgcgc gcacgcgctc ttccaggatt tcctgcggga tttcatcacc
    11881 aataaacgcc gagaggatct tcgcactgcg ggtgacaaaa tccagcttca gcatctcatc
    11941 aatttcagtc aggctgaatt ccggcaggtc gtgcggaaaa aacagcccct gatttttgcc
    12001 caacccctgg gttacggctt gcgcaaagct gacctgctca ttgtgatctt tcagattgta
    12061 gagtttcatt tagttttcca gtactcgtgc gcccgccgta tccagccggc aaatatgaac
    12121 aaaaccttcc tgattttgca ggtagttctt acccaaccag tcggcaacgc gctgggcggt
    12181 atccggcttg tcacacagag cgaacaaggt cgggccggag ccggagatac cgctcgctac
    12241 cgcgccgatt tccgcgaccg cctgccgcgc ctgccggaag ccaggcagta accgttcacg
    12301 gtagggttct gcgataacat ctttcatcag cttcgcggca agctcaggct gacgggaata
    12361 gcaagcgtga atgaagcctg ccagatgtcg cccgtgcgca atgcaatcct ggcggcgata
    12421 ctgcgccggt aaaatagccc gggcttctgc cgtcgagact ttaatccccg gatacgccag
    12481 cacccacagc cactcatcaa accctggcac ttgctggctg atgatgtcgt tttcttcgat
    12541 catcaactgc ataccaccaa gaaaacacgg tgccacgttg tcgtaatgaa tgctgccgga
    12601 gatacgccct tccagctcgc ccatcaaagc cagcaaacga gcgtcattaa gcggcttgcc
    12661 gcagtgttca ttcatcgcca tcagcgccgc gaccaccgaa caggcgctgg agcctaagcc
    12721 cgaaccgatc ggcatattct tttccagagt catcgccact ggaatttgct tgcccagctc
    12781 ctggcaaaaa cgctcccagc actgataaac gatattttcc cgtggttctg acggcagctt
    12841 atcggcaaaa cgtccgaggt tgttgaggct gaatgtctct gccgtctcaa ccgtgactac
    12901 atctccgagc aatgcaccat caacgggtgt caccgccgcc ccgagcacat caaacccgac
    12961 gctcatattg gcactggaag ccggggcata aactttaacc atgtcagact cctagcttcc
    13021 atgagagggt acgtagcaga tcggcaaaga caccggcagc ggtaacgtca ttgcccgcac
    13081 catatccgcg cagcaccaat ggcagcggct gataatagtg gctatagaag gccagggcgt
    13141 tttcgccatt tttcactttg aacagcggat cattaccatc cacttcggca atcttcacgc
    13201 ggcagacgcc atcttcatca atattgccaa catagcgcaa aacttttcct tcatcacggg
    13261 ccttcgccac gcgcgcggca aagagatcgt cgagctgtga cagattcgcc ataaaagcgg
    13321 caacatcacc ctcggcgtta aactctgcgg gcagcactgg ttcaatttca atatccgcca
    13381 actccagttc acgtcccgtt tcacgagcga gaattaatag tttacgcgct acatccatac
    13441 cagaaagatc atctcgcgga tccggttcgg tataacccat ttcccgcgcc agagtagtcg
    13501 cctcggagaa actcatgcct tcgtctaact tgccgaagat ataagaaagc gaacctgaaa
    13561 gaatgccgga gaacttcatc aattcatcac cagcattgag cagattttgc aggttctcaa
    13621 taaccggtaa tccagcccca acgttggtgt catagaggaa tttacgtcgc gatttttccg
    13681 ccgcatgacg caacagatgg tagtaatcca tcgacgaggt gttggccttt ttgttcggcg
    13741 tgacaacgtg gaaaccttcg cgcaggaagt cggcatattg atccgccact gcctggctgg
    13801 aggtgcagtc aacaatgacc gggttcagca gatgatattc tttcacgagg cgaattaagc
    13861 gcccgagatt aaacggctct ttggcttgcg ccagttcttc ctgccagttt tccaggttta
    13921 ggccatgcac attggtaagc agagccttcg agttagcaac accgcagaca cgtaagtcga
    13981 tatgtttatt cttcaaccag ctttgctgac gcttcagttg ctccagcagc gcaccgccaa
    14041 cgccaccgac gccaatcaca aacacttcga taacctgatc ggtattgaac agcatctgat
    14101 gagtaacgcg cacgccagtg gtcgcatcat cgttatttac cacgacagag attgagcgtt
    14161 cagaagatcc ctgagcaatg gcgacaatgt tgatattggc gcgggccagc gcggcaaaga
    14221 atttcgccga gatcccacgc aaggtgcgca taccatcacc taccaccgag ataatggcca
    14281 gccgttccgt caccgccagc ggctccagta agccttcttt cagttccagg tagaactctt
    14341 cctgcattgc ccgttcagct cgcacacagt cgctttgcgg aacgcagaaa ctgatactgt
    14401 attcggaaga tgattgcgta atcagcacca cggaaatacg ggcgcgtgac attgcagcaa
    14461 agacgcgcgc cgccatgccg accattcctt tcatccccgg gccggaaacg ctgaacattg
    14521 ccatgttatt cagattggaa atgcccttga ccggtaattc gtcttcatca cgactggcac
    14581 caatgagcgt acctggtgct tgaggatttc cggtattttt aatcaggcac gggatctgga
    14641 actgggcgat gggggtaatg gtgcgggggt gaagaacttt agcgccgaag taggaaagct
    14701 ccatcgcttc ctggtaggac atcgatttca acaacctcgc atcgggcacc tgacgcgggt
    14761 cgcaggtata aaccccgtca acgtccgtcc aaatctcgca acaatcggcg cgtaaacagg
    14821 cagccagcac cgccgcggag tagtcggaac cgttgcgtcc aagtaccacc agttcgcctt
    14881 tttcattacc ggcggtgaaa cctgccatca gcaccatgtg atcagccgga atacgacttg
    14941 ccgcaatacg gcgggtggac tcagcaatat cgacggtaga ttcgaggtaa tgccccactg
    15001 ccagcagttt ttcgaccgga tcgataacgg taacgttgtg accacgcgct tctaacacgc
    15061 cggccataat ggcgattgac attttctcgc cacggcaaat cagcgcagcg ttaatgctat
    15121 ccgggcactg ccctaacaaa ctaatgccat gcaggacatg ttttatttgg gcaaattcct
    15181 ggtcgacgaa agttttcaat tgcgccagcg ggaatcccgg ctgggcggcg gcgagtcccg
    15241 tcagaagttc ggcaaaaata cgttcggcat cgctgatatt gggtaaagca tcctggccgc
    15301 taatggtttt ttcaatcatc gccaccaggt ggttggttat tttggcgggg gcagagagga
    15361 cggtggccac ctgcccctgc ctggcattgc tttccagaat atcggcaacc cgcagaaaac
    15421 gttctgcatt tgccactgat gtaccgccga acttcaacac tcgcatggtt gttacctcgt
    15481 tacctttggt cgaaaaaaaa gcccgcactg tcaggtgcgg gcttttttct gtgtttcctg
    15541 tacgcgtcag cccgcaccgt tacctgtggt aatggtgatg gtggtggtaa tggtggtgct
    15601 aatgcgtttc atggatgttg tgtactctgt aatttttatc tgtctgtgcg ctatgcctat
    15661 attggttaaa gtatttagtg acctaagtca ataaaatttt aatttactca cggcaggtaa
    15721 ccagttcaga agctgctgtc agagactctt tttttaatcc acacagagac atattgcccg
    15781 ttgcagtcag aatgaaaagc tgaaaaatac ttactaaggc gttttttatt tggtgatatt
    15841 tttttcaata tcatgcagca aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc
    15901 caggcgttgt tgtaaccagt cgaccagttt tatgtcatct gccactgcca gagtcgtcag
    15961 caatgccatg gctcgttcgc gtaaagcttg cagttgatgt tggtctgccg ttgtgtcact
    16021 tttcgtcggt tgttgtatta atgttgctaa ttgatagcaa tagaccatca ccgcctgccc
    16081 aagattgagc gaaggataat ccgccaccat cggcacacca gtaagaacgt cagccaacgc
    16141 taactcttcg ttagtcagcc cggaatcttc gcgaccaaac accagcgcgg catggctcat
    16201 ccatgaagat ttttcctcta acagcggcac cagttctact ggcgtggcgt agtaatgata
    16261 tttcgcccga ctgcgtgcag tggtggcgac agtgaaatcg acatcgtgta acgattcagc
    16321 cagcgtcggg aaaactttaa tattatcaat aatatcacca gatccatgtg cgacccagcg
    16381 agtggctggc tccagatgtg cctgactatc gacaatccgc agttcgctaa accccatcgt
    16441 tttcattgcc cgcgccgctg ccccaatatt ttctgctctg gcgggtgcga ccagaataat
    16501 cgttatacgc atattgccac tcttcttgac caaatgaccg cgaacagggt gatcactgcc
    16561 aacttattac gcggtgcgaa tttacaaatc cttaacgtaa gtcgcagaaa aagcccttta
    16621 cttagcttaa aaaaggctaa actatttcct gactgtacta acggttgaat tgttaaaaaa
    16681 tgctacatat ccttctgttt acttaggata attttataaa aaataaatct cgacaattgg
    16741 attcaccacg tttattagtt gtatgatgca actagttgga ttattaaaat aatgtgacga
    16801 aagctagcat ttagatacga tgatttcatc aaactgttaa cgtgctacaa ttgaacttga
    16861 tatatgtcaa cgaagcgtag ttttattggg tgtccggccc ctcttagcct gttatgttgc
    16921 tgttaaaatg gttaggatga cagccgtttt tgacactgtc gggtcctgag ggaaagtacc
    16981 cacgaccaag ctaatgatgt tgttgacgtt gatggaaagt gcatcaagaa cgcaattacg
    17041 tactttagtc atgttacgcc gatcatgtta atttgcagca tgcatcaggc aggtcaggga
    17101 cttttgtact tcctgtttcg atttagttgg caatttaggt agcaaacatg cagaccccgc
    17161 acattcttat cgttgaagac gagttggtaa cacgcaacac gttgaaaagt attttcgaag
    17221 cggaaggcta tgatgttttc gaagcgacag atggcgcgga aatgcatcag atcctctctg
    17281 aatatgacat caacctggtg atcatggata tcaatctgcc gggtaagaac ggtcttctgt
    17341 tagcgcgtga actgcgcgag caggcgaatg ttgcgttgat gttcctgact ggccgtgaca
    17401 acgaagtcga taaaattctc ggcctcgaaa tcggtgcaga tgactacatc accaaaccgt
    17461 tcaacccgcg tgaactgacg attcgtgcac gcaacctgct gtcccgtacc atgaatctgg
    17521 gtactgtcag cgaagaacgt cgtagcgttg aaagctacaa gttcaatggt tgggaactgg
    17581 acatcaacag ccgttcattg atcggccctg atggcgagca gtacaagctg ccgcgcagcg
    17641 agttccgcgc catgcttcac ttctgtgaaa acccaggcaa aattcagtcc cgtgctgaac
    17701 tgctgaagaa aatgaccggc cgtgagctga aaccgcacga ccgtactgta gacgtgacga
    17761 tccgccgtat tcgtaaacat ttcgaatcta cgccggatac gccggaaatc atcgccacca
    17821 ttcacggtga aggttatcgc ttctgcggtg atctggaaga ttaatcggct ttaccaccgt
    17881 caaaaaaaac ggcgcttttt agcgccgttt ttatttttca accttatttc cagatacgta
    17941 attcatcgtc cgttgtaacg tctttactgg ctttcatttt cggcagtgaa aacgcatacc
    18001 agtcgatatt acgagtcaca aacatcatgc cggccagcgc caccaccagc acactggttc
    18061 ccaacaacag cgcgctatcg gcagagttga gcagtcccca catcacacca tccagcaaca
    18121 acagcgcgag ggtaaacaac atgctgttgc gccagccttt caataccgcc tgcaaataaa
    18181 taccgttcat taacgcccca atcagactgg cgattatcca tgccacggta aaaccggtat
    18241 gttcagaaag cgccagcaag agcaaataaa acatcaccaa tgaaagcccc accagcaaat
    18301 attgcattgg gtgtaagcgt tgcgcggtga gcgtttcaaa aacaaagaac gccataaaag
    18361 tcagcgcaat cagcagaatg gcgtacttag tcgcccggtc cgttaattgg tattgatcgg
    18421 caggcgtcgt cactgcgacg ctaaacgcgg ggaagttttc ccagccggta tcattgcctg
    18481 aagcaaaacg ctcaccgaga ttattagcaa accagctgct ttgccattgc gcctgaaaac
    18541 ctgactcgct aacttcccgt ttggctggca aaaaatcacc taaaaaactg ggatgcggcc
    18601 agttgctggt taaggtcatt tcgctattac gcccggcagg caccacagaa agatcgccgg
    18661 taccgcttaa attcagggcc atattcagct tcaggttctg cttgcgccag tccccttcgg
    18721 gtaaagggat atgcacgccc tgcccgcctt gctctaaccc ggtgccgggt tcaatggtca
    18781 gcgccgttcc gttaacttca ggcgctttca ccacaccaat accacgcgca tccccgacgc
    18841 taatcacaat aaatggcttg ccaaaggtga tatttggcgc gtcgagctcg ccaagacgcg
    18901 aaacatcgaa atcggctttt agcgttaaat cactgtgcca gacctgaccg gtataaatcc
    18961 ctatcttgcg ttcttccacg ttctgattgc catcaaccat caatgactca ggtaaccaaa
    19021 aatggataaa acttcgtttc cgctccacgg ttttatcctc ttcctgcacc gtataaagct
    19081 cggtcacagg aatagcgata agcggcccaa cgagtttttg cggcccactg gtactttggc
    19141 gaatcgcatc ttccacatcg ctacggtaat cggcacgttc gacaatcacc tgccgaatca
    19201 gcattatcgg aatcaacaac agcaatactg caccaaacag ggtggtcatt ttccagaaca
    19261 ggggggattt caacatagtc ttctcctttg caatggcagc aggatacgaa ggctatgtgg
    19321 gaagaatttg aagctatgtg aagtgacggt gaagtcgaag cgaggccagc acgccgcctt
    19381 cctgcacgtt gcgcagcgtg acttcgccgt taaacaaacg ggcgacctca ctgacgaacg
    19441 ccaaccccag accgctgctt ttttgcccat ttgcacgcgg caaagagtaa aagcgttcaa
    19501 aaatacgtga cagcgcgtag tcaggaatac cactaccggt atccagcacc ttaagcgtga
    19561 cgtgttcatg atccacttcg gcgcttagcg tgatgcaacc gctctcggga gtaaaatcga
    19621 tggcgttatc cagtaaattc cccagcgcct gctccagtaa cgccggttca gcagcaacgt
    19681 taacctcagt aggcataaca tgcaaagtga tgtttttttc tgccaactgc acggtgcgcg
    19741 cttcgctgac gcggcgaaat aatgccgcca catcaacagc agtcagaact acttcctgac
    19801 gattctccag tcttgcctgg cgtagtaacg tttctaccaa tgcctgcata cgcgcatttt
    19861 gggtcagaat gttgtcggta aaacgagcca ccacttccgg tggcggacct tcgcgtaaaa
    19921 tttccgccgc gccacgaatc gccgccagtg ggctttttag ctcatgggtt aacgcataaa
    19981 catactgctc aatatagttt ttcccttcca gcttcacgcg catactttcc agcgcctgcg
    20041 caagcttacg cagctcgcta ctgccgagtt caggaagagg aacgggctta ttgtcagtga
    20101 cggaatcggc atagcgagtg agccgggcaa tggagcggtt gatccaccaa accatgcctg
    20161 cgccaatcac cagtgcaatc cccagcaaaa tggcgctggc ccataaaatt cgccgctcgc
    20221 tgcgcttaat gaccggagcc atcgccgcgt tcggtttgcc tacgctcaaa acgccaataa
    20281 gccgcgagcc gtccataatc ggcgcggcaa catacatcac tgaactttcg ggatcggcag
    20341 gattttgcaa cgtgctgcgc gcaccatact gaccacgcaa cgttagccag acgtcattcc
    20401 agcgcgaata atcctgccca acggctttat ttgccgaatc gaacaatact ttgccctgcg
    20461 catcggtcat atagacatga tattcattgc gcactttgtt aatgccaccg atattggcgc
    20521 gaaacgggcg atgttgtagc tgattaaacg cctgcgccag ttgcccatgc gttgggtccc
    20581 cagagagcaa atcgggacgc gccagctccg ccagcaacgt tgcggtgtcg atcagcgtcc
    20641 cctccgttgc tcttcgcacg cccggtttaa cttctttgac aaaaatagcc agtacgaacc
    20701 aggccgccac cgccaccagt aaaaaatagc ccagcaacaa ccgcatgccg atacgcatta
    20761 caggcccctc aggctatatc ccatgccgcg atgagtatta atcggtgaaa gatcggggtt
    20821 gatggcgcgc agcttggcac gcaacgtttt aatgtgggta tcgacggtgc gatcgtaggt
    20881 gtcctgcgca tcttcccaca cgctatccat tagttgctgg cgggaccaga cgcggcctgg
    20941 cgacttgagt aacgtcttca gcaataaaaa ctcataccgc gtcagcgtta atggcgtgtc
    21001 aaaccagctg atctgcgccg cgggttcatt caattcaaaa tgaccaatac ggatgacggg
    21061 agacggcgac gagaacttct tcacccgacg cagtaaggtg cgcaccctgg cgcacacttc
    21121 gcggggtgaa aacggtttag ccacatagtc gtcagcgcca atttccagcc caagcaggcg
    21181 atcgacctct tcacttcggg ccgtcaggaa cagtacaggt aacgccggat ggagcgccag
    21241 taactggcgg cacaattcaa agccgctgat atcaggcaaa ccaacatcga gaatcatgac
    21301 atcgggaacc tgctggcgag ctttatccag caccggcaag cctcgctcaa agacctcgac
    21361 ggcaaaacct tcctgctgca acatgtagac cagcgtgtcg gctatccctt gctcatcttc
    21421 cactaaccag accgtttccc gttgcataaa tcgcctctgt tattgccgcc acggcatgac
    21481 aggaaccgcg ctaatcgcgt ttttcggcga accttctaca actttgtcgg agtaagccag
    21541 ataagcgagc gcgttgcgtt tggcatcata aaagcgcacg acctgtaacg acttaaagac
    21601 cagggacgtg cgttttttga atactacctc gccctgagct ttgccgtttt taatacgatc
    21661 cgacagttca atcggcccga cttgctgaca agaaatggcc gcatcggagg tatcttccgc
    21721 caggcccaat ccccctttaa tacccccggt tttcgcccgg ctcacataac aggtgacatt
    21781 tttcacatcg ggatcgtcaa aggcttccac aacaattttg tgatccgggc cgatcatttt
    21841 aaatacggtg tcgaccgaac caatctcttc tgcatgagcc aatggcccca gcattatcag
    21901 gcttaaagac aggatcaaat gcttgtattt catattgtta ccatttttat ctattacacg
    21961 agcaattagt tcgtcacggt aaataacata ttgattaaga acaaaatctc aatactttta
    22021 tttccgtcaa gacctaaaac atactctact aagaaaaaaa cactgaatgc taaaacagca
    22081 aaaaatgcta ttatccaatt acctgatgtc aggtgctcgt tgttgaaagg atgaggatat
    22141 tttatggatc aggccggcat tattcgcgac cttttaatct ggctggaagg tcatctggat
    22201 cagcccctgt cgctcgacaa tgtagcggcg aaagcaggtt attccaagtg gcacttacag
    22261 agaatgttta aagatgtcac tggccatgct attggcgcgt atattcgtgc tcgtcgtttg
    22321 tcgaaatcgg cggtcgcact acgcctgact gcgcgtccga ttctggacat cgcgctgcaa
    22381 taccgcttcg actctcaaca gacatttacc cgcgcattca agaagcagtt tgcccagact
    22441 cctgcacttt accgccgttc tcctgaatgg agcgcctttg gtattcgccc gccgctgcgt
    22501 ctgggtgaat tcaccatgcc agagcacaaa tttgtcaccc tggaagatac gccgctgatt
    22561 ggtgttaccc agagctactc ctgttcgctg gagcaaatct ctgatttccg ccatgaaatg
    22621 cgttatcagt tctggcacga ttttctcggc aacgcgccga ccattccgcc agtgctctac
    22681 ggcctgaatg aaacgcgtcc gagtcaggat aaagacgacg aacaagaggt attctatacc
    22741 accgcgttag cccaggatca ggcagatggc tatgtactga cggggcatcc ggtgatgctg
    22801 cagggcggcg aatatgtgat gtttacctat gaaggtctgg gaaccggcgt gcaggagttt
    22861 atcctgacgg tatacggaac gtgcatgcca atgctcaacc tgacgcgccg taaaggtcag
    22921 gatattgagc gatactaccc ggcagaagac gccaaagcgg gagatcgccc aattaatctg
    22981 cgctgtgaac tgctgattcc tatccgccgt taacgctgca gctcatctaa tgcaggggcg
    23041 tccagatgcg agatgtcccc tgcggtttca acgacccagc cagacgccag ccacaggctt
    23101 tcctgataat ccacgcgcga aatagaacag ttacgcagac gtaagcggcg ctctgcccat
    23161 gctggtaatc cgagaatcgt actcaccaga catcccagtg caataccatg gctcaccagc
    23221 agcggtcggc ttccctgcgg taagtcccgg caagattcca gtgccgcatt gacgcgatcg
    23281 ctgagctcct gcattgactc gccttcagga atacgcccat caacggtgcc attgaccagc
    23341 tgccgacgcc agttctcttc ttcttcggtc agagaatcga tatgtctttt ttccagcaca
    23401 cccatgttta attcacgcag gcgagaatca aagatgatgt cacagccgca ggcctgggcg
    23461 atgatttccg ccgtacgccg ggtgcgtcct aaatcgctac tgatgatatg cgtaatgcca
    23521 agctctttgg cacgggttgc cacctgcatc gcctgttgct cacctttggc ggtcagcggg
    23581 ctgtcagact ggccctgaat acgtcgctcg gcgttccact gcgtttcacc gtggcggact
    23641 aggtatacct gtaacatgct ttttttccgt atactgctgt aacgttaatt ttctgagtaa
    23701 tactgattat gcaccaagtt gtctgtgcga ccaccaatcc cgctaaaatt caggccattc
    23761 tgcaggcatt tcacgagatc ttcggcgaag gatcctgcca tattgcatcc gttgccgtcg
    23821 agagcggtgt accggaacag ccctttggca gtgaggaaac gcgcgctggc gcacgaaatc
    23881 gggtagcaaa tgcccgccgt ttacttccag aggctgattt ttgggtggcg attgaagctg
    23941 gcattgatgg cgacagcact ttcagttggg tggtgattga aaacgccagc cagcgcggcg
    24001 aggcgcgttc ggctactttg ccgttgcctg cggtgattct ggaaaaagtg cgtgaaggtg
    24061 aagcgcttgg tcccgtgatg tcgcgttata ccggtattga tgagattggt cgtaaggaag
    24121 gggcgatcgg cgtgtttacc gccgggaaac tcactcgcgc cagcgtctat caccaggcgg
    24181 tgatcctggc gctgagtccg tttcataatg ccgtgtatta agcgcctgat gcgacgctgc
    24241 cgcgtcttat catgcctacc aaacatattg aaattacggg tatttgtagg acggataagg
    24301 cgttcacgcc gcatccggca ttcggtgcac gatgcctgat gcgccacgtc ttatcaggcc
    24361 tacaaaatca atcgcttttc agcaacacct cttccagcca ctggcgcagc tcgacgggcg
    24421 cggctttcag gctgttagat ccacgcgtaa tcgtcgcgat gcctgcgccg agttcatttt
    24481 ttaactcacg ctggctcatt tcgccgcgca acagctcttc gacaatacgc acgcgagtcc
    24541 ccaacgcttc gcgctcatct ggcgtcagca tcaggtttaa caacggtaaa tggagatcgt
    24601 tttggtaggc attcttaagc aggtcgacaa aacgtaacca ctcctggtga cgctgttctg
    24661 ccatcgctgc tgaatagggt gattgttggg ccataatatg tcgccattgt tagcggggga
    24721 agcaaaatgc ctcccccggt tgtactcgct aaagagtacg atagcatatc ataaacgtgt
    24781 ggatcagtaa cgacgtcccc attccgtggc gctcatcaac gtcggtttat cccccatgaa
    24841 atagcggtag taagcgtcat aagccagcac gttcttcaca taaccgcgcg tctcggagaa
    24901 tggaatactc tcgacaaatg ccactgcgtc gatacgcccg gcgctgttgc caagccaggt
    24961 tcgcacccgc cctggtccgg cgttataagc tgctgaggag aaaatacgat tattgccaaa
    25021 ttgctgataa acatattgca ggtaactggt gccaatgttg atattcgttt ccggatccag
    25081 caattgccca gggctgctat aaccaggaat agagaacatc ttcaccgtat gggtcgccgt
    25141 gcccggcata atctgcatca ggccgctggc ccctaccggt gatttcactt tcggattcca
    25201 ggcgctctcc tgacgggcaa tcgccatcgc atagctttgc gggatctcct taccgctggt
    25261 gtagcgtttg aaaagatcgt tgtaagccag cgggaatcgc tcttccagat gatcccacaa
    25321 cttcccggcg atcgttgcct gaacgctaag atcccaccat tggttgttga aagcataccg
    25381 cgccagttga gcttgctctg tttttgactt gctcttcacc agattggccc actcgctacg
    25441 cgcggtgtta tcgagattcc agtacatcaa ctcgcgcacg cgcgccatct ccggcccctg
    25501 agtcagggcg ctgtcaacat tctgcggcgc tttatcaatc ttcagctcat actcttctcc
    25561 gatgcgttgc gctgcaacca tcgggtagaa accacgctgt tgcatgagtt gatgcaaaat
    25621 ctcttttgct tcagcttcac gtccgcgttc cagcaataaa tccgcctgcc agtaacgcca
    25681 ttcatctttc tctttcgcct ccatcggcaa acgcgccagc caggtattca ggccgcgacg
    25741 atcgccagtg ccaagcgcca ttcgcacacg gcgttcaatc aacgacgtgg attgcgagcg
    25801 cataatggcg tcatcgcgcc atttcgcctg ctcgtcggtg acatcgttgc ccatcaaacg
    25861 ccaggcgacg atatcgcgca gctcctgaat ctgatcttca ttaagctgct gcgcctgggc
    25921 aagcgacggg atcatcagcc gtgcattttc agcatcctgc cgcgccacac tggcaaacgc
    25981 caccgccgcc atttgacggg taaaatcggt cgctccggtt gtacgcgcga aggtcagtac
    26041 cgtattaggg ttgttcgcca gtgaaatgat tgccgaggcg atagtctggt aatcggcagg
    26101 catctgccct gccagcactg ttaccaggcc agtgttaccc gctttcatcg ccagacggat
    26161 acgctctaaa tacgccagcg gatcttgttt acctgacgca cgccagacgc taaataactt
    26221 gtcacaggcg ttaggctggc tcttgccggt tagccacagc tctttcgccc cttgccaggc
    26281 ttcttcactc tgcccggtgt tccatttcgc atagtagtaa ttacattgcg cttcggtagt
    26341 tccgggcttt tccgggctaa aggctaacaa gccacgccag tcttcacgcc gcgccagttc
    26401 attgacgaaa cgagattgca gcgtgcgagc gggaggaagc gtggggttag cgcgaacaaa
    26461 gttagtgacc gtcaccgccg gttgattcat caggtcatcg gtgatctggc ggtattccag
    26521 gtagggataa agcggataat ccttcagtcc aggcatcatt tgttcgacca catccatttg
    26581 tcgattatcc caggcctgct tgatttgcgc gtaacgacta cgctgctcat ccagtgagtc
    26641 ggctcgcgcc acgctgctga ccgtcagcag acagacaccg gcagccaaca gccgccaggt
    26701 aacttgtttg gctttttcca caagcgcatc ctctaagtgt aaatacatca atgcagcgtc
    26761 atgccgcgta atagatttat gctaaccagt cattgccgtt tacgccacgt tacggacact
    26821 tttttacttt tactgcgaag gtgatggacc aactgggatt aggcggggaa aaaggctaca
    26881 cttcgccttt gaaaactcat tatcacgata aacatagagg cgaagtccaa cgtggctcaa
    26941 ttcgtttata ccatgcatcg tgtcggcaaa gttgttccgc cgaaacgtca tattttgaaa
    27001 aacatctctc tgagtttctt ccctggggca aaaattggtg tcctgggtct gaacggcgcg
    27061 ggtaagtcca ccctgctgcg cattatggca ggcattgata aagacatcga aggtgaagcg
    27121 cgtccgcagc cagacatcaa gattggttat ctgccgcagg aaccgcagct gaacccggaa
    27181 cacaccgtgc gtgagtccat tgaagaagcg gtttcagaag tggttaacgc cctgaaacgc
    27241 ctggatgaag tgtatgcgct gtacgccgat ccggatgccg attttgacaa gctggccgct
    27301 gaacaaggcc gtctggaaga gatcattcag gctcacgacg gtcataatct gaacgtacag
    27361 ctggagcgtg cggcggatgc gctacgtctg ccggactggg acgcgaaaat cgctaacctc
    27421 tccggtggtg agcgtcgtcg cgtggcgttg tgccgcctgc tgctggaaaa accagacatg
    27481 ctgctgctcg acgaaccgac caaccacctg gatgccgaat ccgtggcgtg gctggaacgc
    27541 ttcctgcacg acttcgaagg caccgtggtg gcgattaccc acgaccgtta cttcctcgat
    27601 aacgttgcgg gctggatcct cgaacttgac cgcggtgaag gtattccgtg ggaaggtaac
    27661 tactcctcct ggctggagca gaaagatcag cgtctggcgc aggaagcttc acaagaagcg
    27721 gcgcgtcgta agtcgatcga gaaagagctg gagtgggtac gtcagggaac taaaggccgc
    27781 cagtcgaaag gtaaagcacg tctggcacgc tttgaagagc tgaacagcac cgaataccag
    27841 aaacgtaacg aaaccaacga actgtttatt ccacctggac cgcgtctggg cgataaagtg
    27901 ctggaagtca gcaacctgcg taaatcctac ggcgatcgcc tgctgattga tgacctgagc
    27961 ttctcgatcc cgaaaggggc gatcgtcggg atcatcggtc cgaacggcgc gggtaaatcg
    28021 accttgttcc gtatgatctc cggtcaggaa cagccggaca gcggcaccat cactttgggt
    28081 gaaacggtga aactggcatc ggttgatcag ttccgtgact caatggataa cagcaaaacc
    28141 gtttgggaag aagtttccgg cgggctggat atcatgaaga ttggcaacac cgagatgcca
    28201 agccgcgcct acgttggccg ctttaacttt aaaggggttg atcagggtaa acgcgttggt
    28261 gaactctccg gtggtgagcg cggtcgtctg catctggcga agctgctgca ggttggcggc
    28321 aacatgctgc tgctcgacga accaactaac gacctggata tcgaaaccct gcgcgcactg
    28381 gaaaacgccc tgctggagtt ccctggctgc gcgatggtta tctcgcacga ccgttggttc
    28441 ctcgaccgta ttgccacgca catcctggac taccaggatg aaggtaaagt tgagttcttc
    28501 gaaggtaact ttaccgagta cgaagagtac aagaaacgca cgctgggcgc agacgcgctg
    28561 gagccgaagc gtatcaagta caagcgtatt gcgaagtaat gcgtaaaatg ccggatgcgg
    28621 cgcgaacgcc ttatccggcc tacaaaacca gcaatttcaa tacgttgtaa aactgtaggc
    28681 ctgataagac gcggtaagcg tcgcatcagg cattgagcac cgaatgccgg atgcggcgtg
    28741 aacacctttt ccggtctaca acagccttag catcctcaat ttcgtggcgg gtcacccgtg
    28801 caggtttttt caaaccttgt ttaatctcga cagcctcttc cagagaggtt ttcaactcat
    28861 caaaaaaact cattttatac ctcgcctttg agcagtttca tcacggttat ctctgctccc
    28921 ccatcatctc ccgcaccagc tccacgcagc gcaggaaacg gctgtcgtaa tcttcctctt
    28981 caacccgcac aaattcgata ttgttctctt ccagcatctc caccagcaga ttctggaact
    29041 ctttacgatc caccgaactg ccgaggctgc gtaaaccatc cgccacccac ggcgtgttgt
    29101 tctccagcag gatcaccaga tcgaaacggt attcatcaat cagcgcctgc acgaacggat
    29161 gttcccgccc ttcgtacttt ttgcagaacg cctgagtggt gacaaaatcg gtatcgataa
    29221 atgccacttt attggcatat ttcaccgcaa aatcaatgta ttgagcgtgg cccagcgcga
    29281 ttttatcgta gtcagaatac tgcaatgcga tctcatcacc gccgaggtgt gaaaagacat
    29341 aatcgcggcc atattcccac gcactggtgg tgttgaagat attggcaagt ttgtttacca
    29401 gggtggattt accgctcgac tcgccgccaa ggatcgccac ggtacgcaca aaaaacggct
    29461 tcacttcggt aggaatatat tcccagtagc ggaacgggtt ttcgcggatc tgcgcaccgc
    29521 tgatactcat aaaggtacgt ttcggatcga ccagcaccgt ctcgatcccc agatgttcca
    29581 tatactgtgg cgcatcggct tcttccgagg tgtagatcag atccggctgg atcccttttt
    29641 cagccataaa ctttttgata ccgttgctcc acacatccca gccgtgcgga tacggctcca
    29701 tgccctcttc gttgaaagca tgaatgcgaa tatttttctg atatttaaaa gtttgcaata
    29761 accagcgcag acgatccggc acggtcggct gctgcgacat ggcactgtct tcgaacaacg
    29821 cgcggtcacg ggtatcgtca aaacccataa tgatatgcag ctcgtcaacc tggctacagg
    29881 cgcgctggat aaggtagata tgtccggtat gcagtgggta gaacttaccg aatacgacac
    29941 cgatcgtttt cttctgccgg ggaaactcaa gccccaaaaa acggtgcaac gcctccagct
    30001 tttgcgcgct ggggcttttg attttggcat tcagtaactg gcttaaatac cctttggtca
    30061 taccgctggc atcagctacc tgctgtagcg tgcagccctg ttgcttgatg gcagttttca
    30121 ggtaatcaaa tgacgacata tctccctccg tatttctcat tataagtcgt cgaacacgct
    30181 aagcgcgtcg gagagttttt taacgccaaa aatctgcatc ccttccggcg cttttttcgg
    30241 tacgttagcc gccggaacaa tcgcccggcg aaaaccgtgt ttcgccgctt cagagattcg
    30301 ttcctgaccg ctgggcaccg ggcggatctc ccctgccagc ccgacttcac caaacaccac
    30361 cagatcctgc ggtagcggtc tgtcacgcag gctggaaacc atcgccagca gtaacgctaa
    30421 gtcggcgctg gtttcggtta cctttacgcc gccgaccacg ttcacaaaca catcctgatc
    30481 ggccatttgc agaccaccgt gacggtgcaa gacagccagc aggattgcca gacggttttg
    30541 ttccagcccc actgccacgc ggcgcggatt cgccatcatc gagtgatcga ccagcgcctg
    30601 aatctccacc agcaacggac gcgttccttc ccacaccacc atcacggagc tgccggaggt
    30661 cacttcatct ccgcgactta agaaaattgc cgaagggttg ctgacttcac gcagcccctg
    30721 ttcggtcatc gcgaagacgc ccagctcatt caccgcgccg aagcggtttt tatggctgcg
    30781 caaggtgcga aaacgggagt cggcatcgcc atccaaaagc accgaacagt cgatacagtg
    30841 ttccagcact ttcgggccag ccagcgaacc atctttggtt acgtgcccca ccatgacaat
    30901 cgccacaccg cgcgttttgg cgaagcgtgt caaataagcc gccgtttcac gcacctgcgc
    30961 cacgctacca ggcgatgact gtacatccgc catatgcatc acctggatcg agtcaattac
    31021 catcagcttc ggttgctctt cttcggcaat caggcagatc tgttcgatgc tggtttccga
    31081 caacatattg agattgtcag tcggcaggcc aaggcgatga gcgcgcattg ccacctgttg
    31141 cagcgactct tcgccggtga catacagcgt tttcatctgc tgggccagtt tgcacagcgt
    31201 ttgcagtagc agcgtggatt tccccgcacc agggttaccg ccaatcagaa tggcacttcc
    31261 tggcaccacg ccgccgccta gtacgcggtc gaactcttta aatccggtgg aaaaacgcgg
    31321 cagctcttca aggctgatat cggagagttt ctggactttt gccaccccgg cgctaccggc
    31381 atagccgctg agacgctcgt tacgcgccac cgtaggcgac gcagcaagac gcacctcggt
    31441 gatggtgttc caggcatgac aggcactgca ctgcccctgc cagcgcggat aatcggcccc
    31501 gcattcatta caaacaaagg cgcgttttgg agcttttgcc acggtgtacc tcgttaatgc
    31561 tgtgccgccc gcaggatggc gggcgagcaa ttacttctga ttcaggctgc ctgagaggat
    31621 gcagaatacc cccatcaggt cagcgtgacg gatggtgact tccgcctttt cattcacttt
    31681 tggcttggca tggtaggcaa tccccagccc tgccgctttg atcatcggca ggtcattggc
    31741 tccatcgcca atcgccacgg tctgcgccag cgggatttca tactcctgcg cgaggcgagt
    31801 cagagttttc gctttgtact gcgcgtctac gatgtcgccg atcacattgc cggtaaattt
    31861 accgtccatg atctccagtt cattggctac cacggctgtc aggcgcagct tgtcgcgcag
    31921 gtattcagca aagaaagtaa agccgccgga ggcaatcgcc actttccagc ccagcgtttc
    31981 cagcttgagc accagttgcg ttaagcctgg catcagcggc agattttcac gcacctgttg
    32041 cagaatattg gcgtcagcgc ctttcagcgt cgccacgcgg ctgcgcaggc tggcggtaaa
    32101 atcgagttcg ccgcgcatcg cccgttcggt tacttccgcc accatctcgc ccgttccggc
    32161 cagtttggca atttcatcaa tacattcaat ctggatggcg gtggaatcca tgtccatcac
    32221 cagcaaaccc ggcgtgcgca ggtgcgggat ttttcccagc ggcgcgacat ccagctgcgc
    32281 ttcgtgggcc aggcgtgtag cccgtgcggt gagtgaacct gccagacgaa tcacctgata
    32341 atcttccacg caccaggcgg caacaatcac catcgccgca cccagtttgc tctggtattg
    32401 ggtcagacgc tgtttatcca gcccacgacc atacagcagc cagccgctac gacctgcgtg
    32461 gtaatccagt ggcatcactt catcaccact taatgaaaga ggcagacccg gccataaaga
    32521 gacatcttca ggcaggtcgc accaggtaat gttaggcatt aaggctcctg taaaatcgtt
    32581 cgaagcaggg aaaataacgc atgaggctac cttgtatcca ttgcttctgg caacattaag
    32641 tctcaaattt tcaaagggtg gaagatggct cgcacaaaac tgaaattccg gctacatcgg
    32701 gcagtgattg tcctgttctg tcttgccttg ttagtggcac tgatgcaggg ggcgtcatgg
    32761 tttagtcaaa accaccagcg acagcgtaat ccacagctgg aagaactggc ccgcaccctg
    32821 gcgcgtcagg tgacgctgaa cgttgcaccg ctgatgcgta ccgactcacc ggatgaaaaa
    32881 cgcattcagg cgatcctcga tcagttaacg gatgaaagcc gtatcctcga cgcgggtgtg
    32941 tatgacgaac aaggcgatct tatcgcccgt tctggcgaaa gcgtcgaagt gcgcgaccgg
    33001 ctggcgctcg acggcaaaaa agcgggcggc tattttaacc agcagattgt cgaacccatt
    33061 gccgggaaaa acggaccgct cggctatctg cgcctgacgc tcgacaccca tacgctcgcc
    33121 accgaagccc aacaggtgga taacaccact aacattttac gcctgatgtt gctgctctca
    33181 ctggcaatcg gcgtagtgct gacccgcacg ctgctacagg gtaaacgtac ccgctggcag
    33241 caatcgccct tcctgttaac cgccagcaaa ccggtgccgg aagaggaaga aagcgagaaa
    33301 aaagagtgac ccattactac aagaaaggaa atcgttatgt ccacattacg cctgctcatc
    33361 tctgactctt acgacccgtg gtttaacctg gcggtggaag agtgtatttt tcgccaaatg
    33421 cccgccacgc agcgcgttct gtttctctgg cgcaatgccg acacggtagt aattggtcgc
    33481 gcgcagaacc cgtggaaaga gtgtaatacc cggcggatgg aagaagataa cgtccgcctg
    33541 gcgcggcgca gtagcggtgg cggcgcggtg ttccacgatc tcggcaatac ctgttttacc
    33601 tttatggctg gcaagccgga gtacgataaa accatctcca cgtcgattgt gctcaatgct
    33661 ctgaacgcgc tcggcgtcag cgccgaagcg tccggacgta acgatctggt ggtgaaaacc
    33721 gccgaaggcg accgcaaagt ctcaggatcg gcctatcgcg aaaccaaaga tcgtggcttc
    33781 caccacggca ccttgctgct caatgccgac ctcagccgcc tggcaaacta tctcaatccg
    33841 gataaaaaga aactggcggc gaaaggcatt acctcagtgc gttcccgcgt gaccaacctc
    33901 accgagctgt tgccggggat cacccatgag caggtttgcg aggccataac caaggccttt
    33961 ttcacccatt atggcgagcg tgtagaagcg gaaatcatct ccccagacaa aacgccagac
    34021 ttgccaaact tcgccgaaac ctttgcccgt cagagtagct gggaatggaa cttcggtcag
    34081 gctccggcat tctcgcatct gctggatgaa cgctttacct ggggcggcgt ggaactgcat
    34141 ttcgacgttg aaaaaggcca tatcacccgc gcccaggtgt ttaccgacag cctcaacccc
    34201 gcgccgctgg aagccctcgc cggacgactc caaggctgcc tgtaccgcgc agatatgctg
    34261 caacaggagt gcgaagcgct gttggttgac ttcccggaac aggaaaaaga gctacgggag
    34321 ttatcggcat ggatggctgg ggcggtaagg taactcgctg attaaaatat ccaaggaagg
    34381 aaatatgatc gatctggaaa accaggaacg ggagattatc aatttaatgt tcagccagcg
    34441 tatttcctgg ctagccgccg tgcgtatcag gcataaatta tcgcttgctg aagtaagtaa
    34501 aatgttaggt atctcgatta attctctaaa acagatcgaa aagacggaac ggcttagctc
    34561 aaacatcaaa agcaaaatgg cagaaattta tggttgtcca ccagagttgc tgatatgccc
    34621 ttcctggatg acggctgaac ataaatgaat agattcgtga atagatacta aaaagccgga
    34681 gcagtccccc actccggctt aacacgacaa tcgccttgca gcgaaacaca attactcttt
    34741 atcgcccagc agaacggatt ccagtgcgat tttgatcatg tcgttgaagg tggtctgacg
    34801 ctcagcggca gtggtctgct cgtgagtgcg gatgtggtca gacacggtgc agatggtcag
    34861 ggctttcgcg ccaaattctg cagcgacgcc gtagatacca gccgcttcca tttccacgcc
    34921 gaggatgccg tatttttcca tcacgtcgaa catttcgccg tccggagagt agaacaggtc
    34981 agcggagaac aggttaccca cgcgagcatc aatgcccagt gctttagctg catctactgc
    35041 gttacgcacc atgtcgaagt cagcgatagc tgcgaagtca tggtctttaa agcggatacg
    35101 gttaactttg gaatcggtgc aggcacccat accaatcacc acgtcacgca gttttacgtg
    35161 cggcagaaca gcgccacagg aaccaacgcg gataattttc ttcacgccga aatcggtgat
    35221 cagttctttg gtgtagatgg agcaggacgg gatacccata ccgtgaccca ttacggaaat
    35281 tttgcggcct ttgtaagtac cagtgaagcc cagcataccg cgtacgttgt tcacttcacg
    35341 ggcatcttca aggaaagttt cagcaatata cttcgcacgc agcgggtcgc ctggcatcaa
    35401 aactacgtca gcgaaatcgc ccatttctgc attaatgtgt ggggtagcca ttgttttatc
    35461 ctttttgtga cataacaaag gcggagtcaa cgctccgccc aaatccatca gaacatggct
    35521 ttgccatatt ccatatcaga agtaccaaaa tattttgcca gagtctggcc gatatccgcg
    35581 aaggtttcac ggtgacccag tgagcccggt tttactttcg ggccgtaaac cagtaccgga
    35641 atgtgttcac gcgtgtggtc agtaccggtc caggtcggat cacagccgtg gtcagcggta
    35701 aggatcagga tgtcgtcatc gcgcagcaga gacatcagct ccggcagacg gcggtcgaac
    35761 agttccagac ccgcagcata accggcgacg tcgcgacggt ggccccagga agagtcgaag
    35821 tcaacgaagt tggtgaagac gatggtgtta tcacccgctt ctttcatctc tttgatggtg
    35881 gcgtcaaaca gcgcgtccag gccagtcgct ttcacttttt tggtgatacc gcagttggcg
    35941 tagatgtccg caattttacc gacagaaacc acctggccgt gtttttcatc aaccagtttc
    36001 tgcagcacgg tcggtgctgg cggctcaaca gccaggtcgt gacggttacc ggtacgctgg
    36061 aagttaccgg ctttgtcgcc gataaacgga cgagcgataa cacgaccgat attgtagccg
    36121 ccgttggtca gctcttcacg ggcgatttcg cacagttcgt agagtttatc cagaccgaaa
    36181 gtttcttcat gacaggcaat ctggaacacg gagtcagcgg aggtatagaa aatcggcttg
    36241 ccggttttca tgtgctcttc gcccagttga tccagaatga ccgtaccgga agagtggcag
    36301 ttaccgaggt aacccggcag attagcgcgt tcgaccagtt tatccagcag ctcttgcggg
    36361 aagctgtttt cgtgatcgga gaaatatccc cactcaaaca gaaccgggac accggcgatt
    36421 tcccagtgac cagacggggt atctttaccg gatgacattt cgtgcgccca tgcgtacgcg
    36481 ccgataactt cagcgttgcc gtccattccc gccggaatga aaccggtaga accttcgtgt
    36541 gctttcgcca gccccagacg ggtcagattt ggcagattga gcgggccttt acgaccgtta
    36601 tcagcttcgc ctttggcaca agcttcagcg atatgaccca gggtgtcagc cccgacgtca
    36661 ccaaagcgtt ctgcatcttc tgtagcgccg atgccgaatg agtccagcac cataataaat
    36721 gcacgtttca tatgttctcc gtactcagtg cttcaaattt aaatgatcag atcagtatac
    36781 cgttattcgc tgatacggcg atagacagtt ggtgtgcttt ccggtgcttt atcggcaagt
    36841 ttaattgccg ctttcaccgc tttcgccgct tcctgccagc tgttttcgtc tttcgcgtgg
    36901 ataaccgcca gcggacgctg accgtctacc tggtcgccca gacgtgccat atcagtaaag
    36961 ccgacgctgt aatcgatggt gtcagatgcc tgacggcgtc cgccgcccat tgcaaccact
    37021 gccatcccca gcgcgcgggt atccatttca ctgacaaaac cttcggtatc agcatagact
    37081 gctttcgtca gcatcgctgt cggcagatac ttcgcgtagt tctcaacgaa gtcggtcggg
    37141 cctttttgtg ccgctaccat acgaccaaag acttctgccg ctttaccgtt gtccagcacc
    37201 gcctgcaatt tcgcgcgcgc ttcggcgtca tctttcgcca gtttgccgga gatcagcatc
    37261 tccacacaca gcgccatcgt gacatcaaac agacgcgggt tacgatattc acccgtcagg
    37321 aactgcaccg cttcacgaac ttcaaccgcg ttacctgcac tggaggccag tacctgattc
    37381 atgtcggtga gcagcgcggt ggtgcgcacg ccagcgccgt tagccacgcc aacaatcgct
    37441 tcggcaaggg cttcagagag ttcgtaggtc ggcataaacg cgccgctacc cactttcacg
    37501 tccatcacca gcgcgtccag accttccgcc agtttcttcg ccaggatcga ggcagtgatc
    37561 agcgggatgg agtccacggt tgcggtaata tcgcgggtcg cgtagaaacg tttatctgcc
    37621 ggagccagtg agctggtctg gccaataatc gccacaccga cgtctttaat aatttcgcgg
    37681 aaacggttgt catccgggaa aatgtcgaag ccagggatgg attccagttt gtcgagcgta
    37741 ccgccagtat gaccgaggcc acgaccggag atcatcggaa tatagccgcc gcaggctgcg
    37801 accatcggcc ccaacatcag cgaagtgaca tcgccgacgc caccggtgga gtgtttatca
    37861 acaatcggtc cattcagatg caggcttttc cagtcgagaa cggttcctga atctcgcatc
    37921 gccatggtca gcgagacacg ctcaggcatt gtcatatcgt ggaagaaaat ggtcatcgcg
    37981 agggcggcaa tctgcccttc ggagatagtg ttgtcgcgaa taccgttgat aaagaaacga
    38041 atttcttcat cgctcagcgc atgaccatca cgttttttac gaataatttc ttgtgcgaga
    38101 aacaaggtaa cctcccgagg aaaagagtat gaggcggctt ttgccggatg cgtctacgcc
    38161 ttatccggcc tacgggtaga tacgtctcgt aggcctgata agcgcagcgc atcaggcgta
    38221 aagcatctta cttagtagct gctggcgctc ttaccgtcgc cgtggcccag cgctttcagc
    38281 aggcttgcca gcaggctgga agcgccaaag cggtagtgac gcgcatcagc ccagtcagca
    38341 ccgaacagtt catctgcgat ggcgagatat ttctgcgcat cttccgcagt acgcacgccg
    38401 cccgccggtt tgaaaccaac ggttttttct acgcccatat cacggatcac ttccatcatg
    38461 atgcgcgcgc tttccggcgt cgcgttcaca gccactttac cggtagaggt tttgatgaag
    38521 tccgcacccg ctttgatgga gatttcagac gctttacgga tcagcgcttc gtctttcagt
    38581 tcgccggttt cgatgatcac tttcagcagt acattcgctg ccgcgcaagc ctctttacag
    38641 gctttcacca ggtcaaaacc aacctgctcg ttacccgcca tcagcgcgcg gtacgggaac
    38701 acaacgtcaa cttcatcagc accgtaggcg attgccgcac gggtttctgc cagtgcgatg
    38761 tcgatgtcgt cgttaccgtg tgggaagttg gttaccgtag caatacggat ttccggggtg
    38821 ccctgctctt tcagtgtttt gcgagcaatc gggataaagc gaggatagat acagatagcg
    38881 gcggtattgc cgaccggagt tttggcctga tgacacagag caattacttt ctcgtcggtg
    38941 tcgtcgtcat tcagggtggt caggtccatc aatttcagtg cacgcaggct gcttgctttc
    39001 agatcagtca tttcattctc ctggcttgtc gccaataaaa ttcaccttgc gagtttgtta
    39061 gtattctaac atctactccg caacacactt cgatatacat cacaattaag gaaatctact
    39121 tacaagtttg catcactata atgcgatctg gttcaaataa ttcactttca aatgaatgcg
    39181 tcagtggtgg caaacgcatc aggatcaaag tgaacatcac gaaacttctt acaatggcgc
    39241 atcaccttcg gcataaaagg aaaagaagat gcccacttct catgaaaatg cactgcaaca
    39301 acgttgccag caaattgtca ccagcccagt gcttagcccg gagcagaagc gccattttct
    39361 ggcactggaa gcagaaaaca atctgcctta cccacagctt cctgccgaag cccgccgcgc
    39421 gctggatgaa ggtgtaatct gcgatatgtt tgaaggtcat gcgccgtaca aaccgcgcta
    39481 tgtcttaccc gattacgccc gttttctggc gaacggttcc gaatggctgg agctggaagg
    39541 cgcgaaagat cttgatgacg cactctctct gctgaccatt ctttaccacc acgtaccgtc
    39601 ggtcacatcg atgccggtct acctggggca actggatgcg ttgttgcaac cgtatgttag
    39661 aattctaaca caagacgaga tcgatgttcg aataaaacgt ttctggcgtt acctcgacag
    39721 aaccctgcca gacgccttta tgcacgccaa tatcggcccg tctgattcgc ccattacccg
    39781 cgcgatctta cgtgcagacg cagagttgaa gcaggtttca ccgaacctga cctttatcta
    39841 cgatcctgaa atcacccctg atgacctgct gctggaagtg gcgaagaaca tctgtgaatg
    39901 tagcaaaccg cacatcgcca acggtccggt gcatgataaa attttcacaa aagggggcta
    39961 cgggattgtg agctgttaca actcactgcc gctggcgggt ggtggcagca cgctggtacg
    40021 ccttaacctg aaagccattg ccgagcgcag cgaatcgctg gatgacttct ttacgcgcac
    40081 tctaccgcac tactgccagc agcagatcgc catcatcgat gcgcggtgtg aattcctcta
    40141 tcaacaatca cacttctttg agaatagctt cctggtgaaa gaagggctga ttaaccctga
    40201 acgttttgtg ccaatgtttg gcatgtatgg gctggcggaa gcggttaact tgctgtgtga
    40261 gaaagaaggg attgccgcgc gctacggtaa agaagccgcc gcaaatgaag tgggttatcg
    40321 catcagcgcg caactggcgg agtttgtcgc caataccccc gtgaaatatg gctggcaaaa
    40381 acgcgccatg ttacacgcac agtcggggat cagttccgat atcggcacca cgccgggcgc
    40441 gcgtttgccg tatggcgatg agccagatcc gatcacccat ctgcaaactg tcgccccgca
    40501 tcatgcttat tattattccg gcatcagcga cattctgacg ctcgacgaaa ccatcaaacg
    40561 taatccgcag gcgctggtac agctttgcct cggtgccttt aaagccggaa tgcgtgaatt
    40621 taccgccaat gtcagcggta acgatctggt tcgcgttacc ggttatatgg tgcgtttgtc
    40681 ggatttagaa aaatatcgcg ccgaaggttc acgcaccaac accacctggc tgggcgaaga
    40741 agccgcacgc aacactcgta ttctggaacg ccagccgcgc gtgataagcc atgaacagca
    40801 gatgcgcttt agtcagtaag attatcccct tctcctgcgt tgacgggcca ggcagtcgtc
    40861 tggctctgtt tttgcagggc tgcaatctgc gctgcaaaaa ctgtcacaat ccgtggacga
    40921 tgggacgttg caatgactgt ggggagtgcg tgccacagtg tccgcatcag gcgttgcaga
    40981 ttgttgacgg caaagtggtg tggaacgctg tggtctgcga gcaatgtgat acctgcctga
    41041 agaggtgtcc gcaacatgcc acaccaatgg cgcaatccat gagcgtggag gaggtgctta
    41101 gccatgtgcg caaagcggtg ctgtttattg aagggataac ggtaagcggc ggcgaagcca
    41161 cgacccaact gccgtttgtg gtggcgctgt ttactgctat caaaaacgat ccgcaactgc
    41221 gccacctcac ctgtctggtg gacagtaacg gcatgttgag cgaaaccggc tgggaaaaat
    41281 tgctcccggt gtgcgacggc gcaatgctcg atctcaaagc gtgggggagc gaatgtcatc
    41341 agcaattaac cggacgcgat aatcagcaga ttaagcgcag catctgtttg ctggcagagc
    41401 gcggtaagct ggcggaactg cgtttgctgg taatccctgg ccaggtggat tatttgcaac
    41461 acatcgaaga actggcggcg tttatcaagg gagtcggcga tgttccggta cgcctgaacg
    41521 cgtttcatgc ccacggcgtg tatggcgagg cgcaaagctg ggcgagcgcc acgccggaag
    41581 acgttgagcc gttggctgat gcgttacggg agcgcggggt gagccggtta atatttccgg
    41641 cgctgtattt gtgacatgcc ggatacggcc tacggttcgg cacagacttg taggcatgat
    41701 aagacgcgtc aagcgtcgca tcaggcatta tgcgccgact gccggatgcg gcgtgaacgc
    41761 cttatccggc ctacggttcg gcacaaacct gtaggcatga taagacgcga caagcgccga
    41821 ctgccggatg cggcgtgaac gccttatccg gcctacggtt cggcacagac ttgtaggcat
    41881 gataagacgc gacaagcgtc gcatcaggca ttgtgcgcca actgccggat gcggcgtgaa
    41941 cgccttatcc ggcctacggt tcggcacaaa cctgtaggca tgataagacg cgttaagcgt
    42001 cgcatcaggc attatgcgcc gactgccgga tgctgcctac gaagggcaaa ctacccacga
    42061 acgttaaaca ccgcatacgt gttattaagc aacacttcgg caatctcatc cgcgggttcc
    42121 gggcgtaact cgcaaagcac ggcaaacaca cgggcagcat gctccgggcg gttaggctgc
    42181 ccctgaaaac cgttgagcgg catatccggc gcatcggttt ccagcaacaa cgatgccagc
    42241 ggtaattttg cgatgacatc gcgggtttta ctggcgcgtg gataggtgat agtaccgcct
    42301 acgccgattt tgtagcccag cagtacaaac cgttcagcct gttgctggct gccagaaaat
    42361 ccgtggacaa caccagcacg cggcaaatcg tggcgtttaa gatgcatcgc cagtttgtcg
    42421 tgcgtgcgcc gtgaatgcag gatcaccggc agatcgtagc gtttcgccag tttcagttgt
    42481 tcgtcgagta accactgctg cctctcaaat tgcggatcgt cgccgaagag atccagaccg
    42541 atctccccca ccgccaccac cttcgccgga cgcctttcca gcgcctgctg tagctgatca
    42601 agagacacat cgctatgttt ttccaacata ccaggatgca agcccaatgc ggcatacagc
    42661 ggttgataat tttccgctaa cgcctgcacg cgggcaaaat tttccgcctc tgtcgccgga
    42721 acaatgatct tgcctacgcc cgcttgtgcc gcgcgttgca ggctggcctc ttcatcgcca
    42781 ctaaacggcg ggaaatcaaa atggcagtgg gtgtcgataa aacggcaaat caagccagat
    42841 cctcattgtt aaatgtcggg tcgttcgcct gcggagcgtc gatcacttcg gcaaccagcg
    42901 tatcgttggc gactggcgca ggcggaatga caatcgattc cggcgtcacc actggcacca
    42961 gatggcgggt aagcggcgct ttttcagtta atagcttgcc aaccgttgcc aggaaataac
    43021 gaccgcataa acgcccaagt ttatagtctt cgcgcagcgc cggaatccga ctgccaagcg
    43081 cgatactatg taatggcttc ggcggataaa tttcgaatat ccgcagcttg cccggtggtt
    43141 tctcaataaa ttgctgaatg tcacgatagc tggtttcatg atgctgcacc agattgacca
    43201 gcggctgcag gctactgtca cccagccagc gttccatgcg tttgaaccac tgcggcgtgt
    43261 agtacatttg tgacggcaca gtgcggatga cgaccaacgt tttagcgccc tgccttgccg
    43321 cctctttaac cggaatcgca tcactgatcc cgccatccag gtagttaatg ccttccagcg
    43381 acactccgct acgataaaag ccaggtatcg cactggaggc gcgaatcaca tccagccagt
    43441 tttgtttggt tggtaaaaag taattcggcg cgtagtcatc ctgacgacag gcgcacatat
    43501 aaaacgattt gccgctgtca aacaaccgcg cggcggtatc catttgcaac ggcatctggc
    43561 ttgcggtggc ctccaccagc cagtcgagat cgataagatt tcctccacgg acaaagcgca
    43621 atggatcgaa aaattcgcgt tttgtggtat agcgcatgat gactttgcgc gcgtaaccgg
    43681 gctgattgca gataaacgcc gagaggttct gcgccccggc agatgtgccg agataaagat
    43741 cgaaaggatt aaactgcgcg cgcataaact catccagcac gccagccgtg aaaattccac
    43801 gctgcccgcc gccttcgcac accagagcaa ttcgtccggg ctggaacggc cttagcgaca
    43861 acggcgcaat attaccaagc gttacaggta ttcgctgccc cacctctgcc ttcctgtttt
    43921 tgttgtgaaa gaatgacaaa gtaacgcaat ttattattcg ctaaaaccgc aaaagccagt
    43981 ccgctggact ggctttggat aaaattttct taatggtctg aaatctaggg tcgttttcgg
    44041 cccatgaaca aactcaccag gaacagaata atcccgacga caaagacaat ttttgctgcg
    44101 cctgcagcgg taccggccag accaccaaat ccaagtgcgg cggcgattaa cgcgataacc
    44161 agaaatatga tgccccaacg aaacataagc gtctccttta ccatagttaa tgtcaccgct
    44221 tgatatgagc gtgaaaatca ctcaatgcga tatttttagt gtggtgcaca tcgcgcctcc
    44281 cgacaaagtt cgggaggacg aattacgacg aattacttag ttttcagatc atttttaacg
    44341 cttttcacac catctaccgc tttggcgata ctttcagcac ggtcactttg tgcctgagaa
    44401 tctacggtac cggagagctg aaccacgcca tcggtggttt caactttcac atgacgggaa
    44461 gggacgatat cgtccgccag cagtttggct ttgatttcac tggtggtggc ggtgtcaccc
    44521 gcgtagccct tcaccgagcc ttctttagcg tcgcgaacgt gcagtttgtc gctgacagag
    44581 gtcacccctt caacgccttt cgccactttc actgcctctt cggcctgggc ctggctttca
    44641 acgaaaccgc tcagggtcac gactttttga tcggttttga cagagatatc ggtgctcttg
    44701 atgttgtcat gatccaccag ggccgccttc actttcgcgg tgatggcgct gtcatccatg
    44761 aaattaccga ctttattcat agagctatcg actttttgcc ctgcgctttc attggtagtc
    44821 tgtgcgttgt tttccgcgta ggcagagccg gtcgcgacgg cagaggtcaa cattacagcc
    44881 agcagagttt tcgaaatctt cagtcttgtc atagtcatcg atttattcct gtatgtttgc
    44941 tcgtaatttg agcctggcaa cacgaggttg cattgctgaa tagggagaga cttcaccctc
    45001 tacagaagtc aatggtcgcc atcacaaaag cgatgagtga tgaataacga ccattacagc
    45061 ctctgaatca gttattaata tcggtagaat gacaatcgac ggctttagat actgatatct
    45121 acgcattgaa cggtatttaa cgccgtcaga aatgtcatca ctttgttaaa tatagatcac
    45181 aattttgaaa ccgctcggga tatcagcgaa aacataagca aaagtgaatg ttttaagaac
    45241 attccgtaag cggctaataa ggaagggaaa ttgacagggc gcagcggcta ccgcgccctg
    45301 gaggcaagaa ttaatgctcg cgggtctggt ggaactgaac gtccggataa cgttcctgtg
    45361 ccaggcgcag gttgaccatg ctggtagcga tgtaagcgag gttatcgccg ccatcaagcg
    45421 ccagttggct ttcgttctta cgcttgaact cttcgaattt cttcgcgtct gcacattcta
    45481 cccagcgggc agtggcaacg ttgactgact catacactgc ttcaacgttg tattcgctct
    45541 tcaggcgcgc taccaccaca tcaaactgca gcacaccaac tgcaccaacg atcagatcgt
    45601 tgttggagat tggacggaac acctgcaccg cgccctcttc ggaaagctgt accagccctt
    45661 tgagcagctg tttttgcttc agcggatctt tcaggcggat acgacggaac agttctggtg
    45721 cgaagttcgg aataccggtg aacttcatca tctcaccctg ggtaaaggtg tcgccgatct
    45781 gaatggtgcc gtggttgtgc aggccgagga tatcgcccgg atacgcttct tcaacgtgcg
    45841 aacggtcacc cgccataaag gtcagcgcgt cggagatcac cacatctttc gcagtgcgca
    45901 cctggcgcag tttcatgcct ttttcatatt taccggacac cacacgcata aacgccacgc
    45961 ggtcgcggtg tttcgggtcc atgttggcct gaattttaaa tacgaagccg gtaaatttat
    46021 cttcgctcgc ttctacggta cgggtatcgg tctgacgcgg catcggcgca ggcgcccact
    46081 ccaccaggcc atccagcata tgatcaacgc cgaagttacc cagcgcagta ccgaagaata
    46141 ccggagtgat ttcgcccgca aggaacagct ctttgtcgaa ctcgttagac gcgcctttaa
    46201 ccagttccag ttcgtcacgc agctgctgtg ccagatcttc accaaccgca gcatcgagat
    46261 ccgggttatt cagcccttta acaatgcgga cttcctgaat ggtgtgccct ttaccgctct
    46321 gatagagata ggtttcatct ttataaaggt ggtaaacgcc tttaaacagc ttgccgcagc
    46381 caattggcca ggtgatcggt gcgcagccaa ttttcagctc gttctcaact tcatcgagca
    46441 attccatcgg gtcgcggata tcacggtcaa gtttgttcat aaaggtgagg atcggcgtgt
    46501 cgcgcagacg ggtaacttcc atcagcttac gggtacgatc ttcaacacct tttgcggcgt
    46561 cgataaccat caggcagcag tccaccgccg tcagggtacg ataggtatct tccgagaagt
    46621 cttcgtgccc cggggtgtcg agcaggttaa ccaggcaatc gtgatacgga aactgcatca
    46681 cagacgtagt aatggagatc ccacgctgct tttccatctc catccagtcc gacttagcgt
    46741 gctggttgga accacggcct tttactgtac cggcagtctg aatggcctgt ccgaacagca
    46801 gcaccttctc ggtgatggta gtcttaccgg cgtccgggtg agaaataatt gcaaaggttc
    46861 ttcttttcgc tacctctgcg gccaaaaggc ttgtcataat tgttgtctct ttgattatgt
    46921 acaatcatat gtacaaagaa aaattcatgc cgctgattgt acattttgga ggtatcagag
    46981 catagcagaa catgatcagt aggtgtattt gtagccaaag aacatttgta catgcagcaa
    47041 tctttgttct attgacactg tttattcata cagttaaaaa taacactgta tacaaacaca
    47101 gtgtagaggg atttttatgc gtattgagat ctgcatagcc aaagagaaaa tgactaaaat
    47161 gccaaacggt gctgtggatg cgttaaagga agaattaact cgacgcatca gtaagcgtta
    47221 tgacgatgta gaggtgatcg taaaagccac cagcaacgat ggcctttctg ttacgcgcac
    47281 cgccgataaa gattcagcta aaacttttgt tcaggaaact ctgaaagata cctgggagtc
    47341 tgctgacgag tggtttgttc actaattaac acgtaaaatc ggtaacggct ggaaatcatt
    47401 caatactcgc actatcggaa gttcaccagc caaccgcagc acgttcttgc atacgacgtg
    47461 tctgcggttt ttcaaccatt cagatacagc actctcatca cattcattct tccgcattaa
    47521 agtatatatg accatatcac cttaatattt ttcttcttca gataaaaatt gttatctatg
    47581 tatactttta aaccgaatcc gtgtagagtc tctacctaag ttagttcgca gctacccctt
    47641 cagtttgcgc cattaacctc ctgacttttg ccgctatctg taagcattcc tgtatacctg
    47701 aaactacagg gttcatctac gaactttaag aacacccagg ata
//
LOCUS       DAZFKF010000029        47562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFKF010000029 DAZFKF010000000
VERSION     DAZFKF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.029"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            133..579
                     /gene="frvA"
                     /locus_tag="ACHKA1_002039"
     CDS             133..579
                     /gene="frvA"
                     /locus_tag="ACHKA1_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO5679695.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            590..2041
                     /gene="frvB"
                     /locus_tag="ACHKA1_002040"
     CDS             590..2041
                     /gene="frvB"
                     /locus_tag="ACHKA1_002040"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO5679696.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2031..3101
                     /gene="frvX"
                     /locus_tag="ACHKA1_002041"
     CDS             2031..3101
                     /gene="frvX"
                     /locus_tag="ACHKA1_002041"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5679697.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAKKRGVEIGNFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NGLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLMLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            3101..4849
                     /gene="frvR"
                     /locus_tag="ACHKA1_002042"
     CDS             3101..4849
                     /gene="frvR"
                     /locus_tag="ACHKA1_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO5679698.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTVCDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAETI
                     VGLTGYEGFMALLRQG"
     gene            complement(4951..6006)
                     /gene="yiiG"
                     /locus_tag="ACHKA1_002043"
     CDS             complement(4951..6006)
                     /gene="yiiG"
                     /locus_tag="ACHKA1_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO5679699.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETLPPANSQPAAPAP
                     EAKPTEAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADW
                     LKDFKQGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAV
                     ALGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKR
                     QLAELKNIEEREGKTFHYYSLAVMISAKQINNLISQDEFDAEAAMKKVSELETLVAQA
                     KEADKGGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFP
                     RALREYNEMVDDYNRLR"
     gene            complement(6159..6992)
                     /gene="fdhD"
                     /locus_tag="ACHKA1_002044"
     CDS             complement(6159..6992)
                     /gene="fdhD"
                     /locus_tag="ACHKA1_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO5679700.1"
                     /translation="MKKTQQKEIENVTNVTGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7186..10236
                     /gene="fdnG"
                     /locus_tag="ACHKA1_002045"
     CDS             7186..10236
                     /gene="fdnG"
                     /locus_tag="ACHKA1_002045"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:7771..7773,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO5679701.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            10249..11151
                     /gene="fdoH"
                     /locus_tag="ACHKA1_002046"
     CDS             10249..11151
                     /gene="fdoH"
                     /locus_tag="ACHKA1_002046"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO5679702.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            11148..11783
                     /gene="fdoI"
                     /locus_tag="ACHKA1_002047"
     CDS             11148..11783
                     /gene="fdoI"
                     /locus_tag="ACHKA1_002047"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO5679703.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            11780..12709
                     /gene="fdhE"
                     /locus_tag="ACHKA1_002048"
     CDS             11780..12709
                     /gene="fdhE"
                     /locus_tag="ACHKA1_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO5679704.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(13039..13281)
                     /gene="yiiF"
                     /locus_tag="ACHKA1_002049"
     CDS             complement(13039..13281)
                     /gene="yiiF"
                     /locus_tag="ACHKA1_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO5679705.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVD
                     QYLVNQSQTVRTSAFGIWQGREEDGVEYQRKLREEW"
     gene            complement(13498..13716)
                     /gene="yiiE"
                     /locus_tag="ACHKA1_002050"
     CDS             complement(13498..13716)
                     /gene="yiiE"
                     /locus_tag="ACHKA1_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO5679706.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(14410..15324)
                     /locus_tag="ACHKA1_002051"
     CDS             complement(14410..15324)
                     /locus_tag="ACHKA1_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5679707.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLTCEAHSHRSVALAKTVAAPIMATG
                     VGAALMYATSSGPYYAQSLSGSPDIEMIKNRMAKLFNQSDTSVRNKYRPAFDKWNELV
                     GKLNHERNTVPFACLTTILATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LFEKRLADDGEIENLFDQQGYFSCDSLTAEEVMMLKDLFDIRVLHLSNKFNVGLQGNI
                     NTDVLQDSFFKNVDFIHESNSYTVGMGV"
     gene            complement(15337..16224)
                     /locus_tag="ACHKA1_002052"
                     /pseudo
     CDS             complement(15337..16224)
                     /locus_tag="ACHKA1_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16634..17575)
                     /gene="fabY"
                     /locus_tag="ACHKA1_002053"
                     /gene_synonym="yiiD"
     CDS             complement(16634..17575)
                     /gene="fabY"
                     /locus_tag="ACHKA1_002053"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO5679708.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(17620..18057)
                     /gene="dtd"
                     /locus_tag="ACHKA1_002054"
     CDS             complement(17620..18057)
                     /gene="dtd"
                     /locus_tag="ACHKA1_002054"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO5679709.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(18054..18926)
                     /gene="yihY"
                     /locus_tag="ACHKA1_002055"
     CDS             complement(18054..18926)
                     /gene="yihY"
                     /locus_tag="ACHKA1_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO5679710.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(18920..19519)
                     /gene="yihX"
                     /locus_tag="ACHKA1_002056"
     CDS             complement(18920..19519)
                     /gene="yihX"
                     /locus_tag="ACHKA1_002056"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO5679711.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TAFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(19660..20547)
                     /locus_tag="ACHKA1_002057"
     CDS             complement(19660..20547)
                     /locus_tag="ACHKA1_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO5679712.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQVGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(20537..21886)
                     /locus_tag="ACHKA1_002058"
     CDS             complement(20537..21886)
                     /locus_tag="ACHKA1_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5679713.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(21985..23418)
                     /locus_tag="ACHKA1_002059"
     CDS             complement(21985..23418)
                     /locus_tag="ACHKA1_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5679714.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASSTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNTLCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLIHWEPLGANVDAEDAEVLAELKK"
     gene            complement(23488..24498)
                     /locus_tag="ACHKA1_002060"
     CDS             complement(23488..24498)
                     /locus_tag="ACHKA1_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFO5679715.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(24531..25418)
                     /locus_tag="ACHKA1_002061"
     CDS             complement(24531..25418)
                     /locus_tag="ACHKA1_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO5679716.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQIMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(25443..26321)
                     /gene="yihU"
                     /locus_tag="ACHKA1_002062"
     CDS             complement(25443..26321)
                     /gene="yihU"
                     /locus_tag="ACHKA1_002062"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO5679717.1"
                     /translation="MATIAFLGLGQMGSPMANNLLHKGHSLQVFDVNALAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            26494..27390
                     /locus_tag="ACHKA1_002063"
     CDS             26494..27390
                     /locus_tag="ACHKA1_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5679718.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIARLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            27430..28218
                     /locus_tag="ACHKA1_002064"
     CDS             27430..28218
                     /locus_tag="ACHKA1_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5679719.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(28329..29594)
                     /gene="yihN"
                     /locus_tag="ACHKA1_002065"
     CDS             complement(28329..29594)
                     /gene="yihN"
                     /locus_tag="ACHKA1_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5679720.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVKLKKEDGASQNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(29696..30676)
                     /gene="yihM"
                     /locus_tag="ACHKA1_002066"
     CDS             complement(29696..30676)
                     /gene="yihM"
                     /locus_tag="ACHKA1_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO5679721.1"
                     /translation="MMTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKKSIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNN"
     gene            complement(30684..31394)
                     /gene="yihL"
                     /locus_tag="ACHKA1_002067"
     CDS             complement(30684..31394)
                     /gene="yihL"
                     /locus_tag="ACHKA1_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5679722.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(31611..33434)
                     /gene="typA"
                     /locus_tag="ACHKA1_002068"
     CDS             complement(31611..33434)
                     /gene="typA"
                     /locus_tag="ACHKA1_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO5679723.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            33807..35216
                     /gene="glnA"
                     /locus_tag="ACHKA1_002069"
     CDS             33807..35216
                     /gene="glnA"
                     /locus_tag="ACHKA1_002069"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO5679724.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            35390..36439
                     /gene="glnL"
                     /locus_tag="ACHKA1_002070"
     CDS             35390..36439
                     /gene="glnL"
                     /locus_tag="ACHKA1_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO5679725.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            36451..37860
                     /gene="glnG"
                     /locus_tag="ACHKA1_002071"
     CDS             36451..37860
                     /gene="glnG"
                     /locus_tag="ACHKA1_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO5679726.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            37972..38082
                     /gene="yshB"
                     /locus_tag="ACHKA1_002072"
     CDS             37972..38082
                     /gene="yshB"
                     /locus_tag="ACHKA1_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO5679727.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(38272..39645)
                     /gene="hemN"
                     /locus_tag="ACHKA1_002073"
     CDS             complement(38272..39645)
                     /gene="hemN"
                     /locus_tag="ACHKA1_002073"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO5679728.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(39834..40340)
                     /gene="yihI"
                     /locus_tag="ACHKA1_002074"
     CDS             complement(39834..40340)
                     /gene="yihI"
                     /locus_tag="ACHKA1_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO5679729.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNNTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(40459..40625)
                     /locus_tag="ACHKA1_002075"
                     /pseudo
     CDS             complement(40459..40625)
                     /locus_tag="ACHKA1_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            40922..41554
                     /gene="yihA"
                     /locus_tag="ACHKA1_002076"
     CDS             40922..41554
                     /gene="yihA"
                     /locus_tag="ACHKA1_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO5679730.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(41936..44722)
                     /gene="polA"
                     /locus_tag="ACHKA1_002077"
     CDS             complement(41936..44722)
                     /gene="polA"
                     /locus_tag="ACHKA1_002077"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO5679731.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIESGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALDEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAYEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LKYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHRDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            45086..46018
                     /gene="yihG"
                     /locus_tag="ACHKA1_002078"
     CDS             45086..46018
                     /gene="yihG"
                     /locus_tag="ACHKA1_002078"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO5679732.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRHDK"
     gene            complement(46059..47489)
                     /gene="yihF"
                     /locus_tag="ACHKA1_002079"
     CDS             complement(46059..47489)
                     /gene="yihF"
                     /locus_tag="ACHKA1_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO5679733.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQISFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAS
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEMNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLHY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT        11943 a        12135 c        11463 g        12021 t
ORIGIN      
        1 atgtgcctta cgccgcaaaa tttatccttc actctgcttt atattgtcac gacttcacga
       61 catatccgcg ctcatcacaa acccgccccg ctattacaat taactgaaat attttttcat
      121 ggagtccgat taatggcagc tcttactgca agctgtattg acctgaatat tcagggcaat
      181 ggcgcttatt ccattctgaa gcagttagcg acaatggcgt tacaaaacgg ttttatcacc
      241 gactcacacc agttcctgca aaccctgctg ctgcgcgaaa aaatgcactc caccggtttt
      301 ggttctggtg tcgccgtgcc gcacggtaaa agcgcctgcg ttaaacaacc gttcgtgtta
      361 ttcgcccgca aagcgcaggc tattgactgg aaagccagcg acggcgaaga cgtcaattgc
      421 tggatctgcc tcggcgtgcc gcaaagtggc gaagaggatc aggtcaaaat catcggcaca
      481 ctgtgtcgca aaattattca ccaggatttt attcaccaac tgcaacaggg cgacgccgac
      541 caagtgcttg ccttgttaaa tcaaaccctc agctcataag gaagtggcga tggagtcatc
      601 cttacgtatt gtcgcgatca ccaactgccc cgccgggatc gctcacacct acatggtggc
      661 ggaagccctg gaacagaaag cccgttctct cggccatacc ataaaagtgg aaactcaagg
      721 gtccagcggc gttgaaaacc gcttatccag cgaagagatt gccgctgccg attacgtgat
      781 tctcgccacc gggcgtggcc tgagcggtga cgatcgcgca cggtttgccg ggaaaaaagt
      841 ttatgagatt gccatctccc aggcgctgaa aaatatcgat cagattttca gtgaattacc
      901 gacaaattcg cagctttttg ccgcagatag cggcgtgaag ctaggcaaac aggaagtaca
      961 aagcggcagc gtgatgagcc acctgatggc gggcgtctct gcggcgctgc cgtttgtcat
     1021 cggcggtggt atcctggtgg cgcttgccaa catgctggtg cagttcggtt tgccgtatac
     1081 cgatatgtcg aaaggcgcgc cgtcgtttac ctgggtagtt gaatccatcg gttaccttgg
     1141 cttcaccttt atgattccca tcatgggcgc ttatattgcc tcgtcgattg ccgataaacc
     1201 ggctttcgct ccggcgtttc tggtttgcta tctggcgaac gacaaagcgc tgctcggcac
     1261 ccagtcgggt gcgggattcc tcggcgcggt ggtgctgggg ctggcgattg gctatttcgt
     1321 cttctggttc cgtaaagtcc gtctcggcaa agcgctacaa ccgctgctcg gttcaatgct
     1381 gatcccgttc gttactctgc tggttttcgg cgtgctcact tactacgtta tcggaccggt
     1441 gatgtccgac ctcatgggcg ggctactgca cttcctcaat accattcctc catcaatgaa
     1501 gtttgcggca gcgtttctgg tcggtgcgat gctggcgttc gatatgggcg gccccatcaa
     1561 taaaaccgcc tggttcttct gcttctcact gctggaaaaa cacatttatg actggtacgc
     1621 catcgtcggc gttgtcgcgc tgatgccgcc cgtcgcggcg ggtcttgcga cgttcattgc
     1681 gcccaaactg tttacccgac aggaaaaaga ggctgccagt agtgccattg tggtcggtgc
     1741 cacagtcgca accgaaccgg ctattcctta cgctctggca gctccgttgc cgatgattac
     1801 cgccaatacg ctggcgggcg gtattaccgg cgtgctggtg atcgctttcg gaatcaaacg
     1861 tctggcacct ggtttgggta tctttgaccc gctgatcggc ctgatgtcgc cggtgggttc
     1921 gttttatctg gtgctggcga ttggcctcgc gctgaacatt tcattcatta tcgtcctgaa
     1981 aggattgtgg ttacgccgca aagcgaaagc cgcgcaacag gagcttgtcc atgaacattg
     2041 agttactgca acagttgtgc gaagccagcg ccgtcagcgg cgatgaacag gaagttcgcg
     2101 acattctgat aaacacgctg gaaccttgcg ttaatgagat cacctttgat ggtctgggca
     2161 gctttgttgc ccgtaaggga aataaaggtc caaaagttgc cgttgtcggg catatggatg
     2221 aagtcggctt tatggtcacc cacatcgacg agagcggttt tctgcgcttt accaccattg
     2281 gcggctggtg gaatcagtcg atgctcaacc accgggtaac aatacgcaca cacaagggag
     2341 tgaaaatccc tggtgtgatt ggttccgtag cgcctcatgc gttaacggaa aagcaaaagc
     2401 aacaaccgct gtcatttgat gagatgttca ttgatattgg cgcgaacagt cgcgaagaag
     2461 cgaaaaaacg cggcgtggaa attggtaatt ttattagccc ggaggccaat tttgcctgct
     2521 ggggcgaaga taaagtagtc ggcaaggcgc tggataaccg catcggctgc gcgatgatgg
     2581 cggagctact acagacagta aataacccag aaattacgct gtacggcgtc ggcagcgtgg
     2641 aagaagaagt tgggctacgc ggggcacaaa cctcggctga acacactaaa ccggatgtgg
     2701 tgatcgtgct ggataccgcc gtcgcgggcg atgttccggg cattgataac attaaatacc
     2761 cgctgaaact gggccagggg ccggggctga tgctgtttga caagcgctac ttccccaacc
     2821 agaaactggt agcagcgtta aaaaactgtg ccgcacataa cggtttaccg ctgcaatttt
     2881 ccaccatgaa aaccggagcg acggatggcg ggcgctacaa cgtgatgggc ggcgggcgtc
     2941 cggttgtcgc gctgtgtctg ccaactcgtt atctgcacgc caacagcggc atgatttcaa
     3001 aagccgatta cgatgctctg ctcatgctga tacggggttt tctgacgacc ttaaccgcgg
     3061 agaaagtcaa cgcgtttagt cagttccgtc aggtggatta atgctaaacg aacgccagtt
     3121 aaagattgtc gatctgctgg agcaacagcc gcgcacgcct ggcgagctgg cgcaacagac
     3181 tggcgtttca ggcaggacca tcctgcgtga tattgactat ctcaacttca cccttaacgg
     3241 caaagcccgc attttcgcca gtggcagtgc gggctatcag ctggaaatct tcgagcgccg
     3301 cagttttttt cagttgctgc aaaagcacga taacgacgat cggctgctgg cgctgttatt
     3361 actgaatact ttcactcccc gtgcgcaact cgcctcggcg cttaatttgc cagaaacgtg
     3421 ggtagcagag cgtctgcccc gcctgaaaca gcgttatgaa cgtacttgct gcctggccag
     3481 tcgccccggt ttgggccatt tcattgatga aacagaagag aaacgcgtta tcttgctggc
     3541 gaacttgcta cgcaaagatc cgtttttaat accgctggcg ggcgtaacac gagataacct
     3601 tcagcattta tccacggtct gcgacaacca acaccgctgg ccgctgatgc agggtgatta
     3661 tctctccagc ctgattctgg cgatttacgc cctgcgtaat caactgaccg atgagtggcc
     3721 gcaatatccc ggtaacgaga taaaacaaat cgttgaacag agcggcctgt ttcttggtga
     3781 taatgctgta agaaccctga cgggtttgat agagaaacag catcagcaag cgcaggtaat
     3841 ttctgccgat catgtgctgg ggttgctgca aagggtgcca ggcatcgcgt cattgaatat
     3901 tattgatacg cagttggttg agaatattac cgggcattta ttacgttgcc ttgccgcacc
     3961 ggtgtggatt gccgagcacc gccaaagcag catgaataac ctgaaagccg cctggcctgc
     4021 ggcctttgat atgagtctgc actttattac gctactgcga gaacagctcg atattcctct
     4081 tttcgacagc gatctgatcg gtttgtattt tgcctgtgcg ctggaacgac atcaaaacga
     4141 acgccagccg atcattttgc tctcggacca gaacgcgatt gccaccatta atcagctcgc
     4201 cattgagcgc gatgttttac attgtcgggt gattattgcc cgtagcttaa gcgagcttgt
     4261 tgccattcgc gaagagattg agccgttatt gatcattaac aacagccatt atttactgga
     4321 tgacgcggta aataattaca tcaccgtaaa aaatatcatt acggctgccg gtatcgaaca
     4381 aataaaacat tttctggcga cggcatttat tcgccagcaa ccggagcgtt ttttctctgc
     4441 ccctggaagt tttcattatt cgaatgtacg cggtgaaagc tggcaacata ttacccggca
     4501 aatttgtgcg caattagtcg cacaacacca tattaccgcc gatgaagcgc aacggatcat
     4561 cgcccgcgaa ggcgaaggtg aaaacctgat tgttaatcgc ctcgccatcc cacattgctg
     4621 gagcgaacag gagcgacgtt ttcgtggatt ttttattacc ctcgcccaac cagttgaggt
     4681 gaataacgaa gtcattaacc atgtcttgat cgcctgcgcc gccgccgatg cgcgtcatga
     4741 gctaaaaata tttagctatc tggcaagcgt attgtgtcag catccggcgg agactattgt
     4801 cggactaacg gggtatgaag ggtttatggc gttacttcgc caggggtgaa tgatgtcgga
     4861 tgcaccgcta acacggctta acgacgttta ggtcggataa ggcgtttacg ccgcatccga
     4921 ccttgtattt ctcagcaaaa acaaaaacgt ttaacgcagg cgattataat catcgaccat
     4981 ttcgttgtac tcgcgtaatg ctctcgggaa agagtcttcg accatccagc ttgagtttgc
     5041 gtcctgaagt tgctctttat cccagtcaga gtacgggact ttatctctga tgcggcgaac
     5101 gtatttttta gcctcgagtt gatactggcc tgccgaatta ataaacgaga aattcatgcc
     5161 gcctttatcc gcttctttgg cctgcgccac cagcgtttcc agttcagaca ctttcttcat
     5221 tgccgcttct gcatcaaact catcttgcga tatcaggtta ttaatttgtt tcgctgaaat
     5281 catgactgcc agagagtagt agtggaatgt tttcccttct ctttcttcaa tatttttcag
     5341 ctccgcaagc tggcgcttat cattaatttc ctgaatcgcc gcatgataag attcggctac
     5401 aggttcaaag gcttccagat tttttaagaa tgtctggtgc agcgttttcc ctttcgcaaa
     5461 cgcatcgtct ttataatttt cctgggtgta atatttatcc atttcgttaa tggtattacc
     5521 caacgccacg gcagcatcga tataactcac cgctacgcca tcaataggtt gcagcgcagg
     5581 cgttaacgcc acagcacttt ttacgccttt ttcacactca gcgaggttgg attcactaat
     5641 gccgtagatg ccataaacag tacgctcttc accggtaggc ccctgtttaa aatctttcag
     5701 ccagtcagca taacgcgcca gactgcgctg taccgggatc tgtaacttgt tgtagcactt
     5761 gatatagaca tccatttttt cgtcgaaaac cgcttgttca tcgaccaccg gttgggcagg
     5821 cgtttcaggt ttagcttctg cttttgcaac gggagcttca gtaggtttcg cttcaggagc
     5881 tggtgcggca ggttgactat tggcaggcgg gagcgtttct gtttcggctt ttttatcatc
     5941 acaacccgtc agaccgaggg ccatgatggc gactataata gcggaagata ataaattcct
     6001 tttcatttaa ttgccttata aatatataga taagagagtg aacataataa atgaaaaaag
     6061 aaaacgccac tacacgcgtt ttatttataa ataaaacaat aaaattcacg aaatgaataa
     6121 gtcctgcaat ttaatatata tcgcaggact tattttaatt aattgctcaa acgctgcgga
     6181 tgggtataaa ccgttgcccg acccggttta caaaaaccta ccagtgtcaa attacagcgc
     6241 tcagccactt ctacagcaag cgtggtcgcg gcagacaccg caaacaaaat ctctacgccg
     6301 cacatcgccg acttttgcac catttcataa ctggcacgac tggaaaccag taccgcacct
     6361 tgctgccagc tttccccttc ttgtgaccgg cggcctaaca gtttgtccag cgctacatgg
     6421 cgacccacgt cttcatgccc gccgaccagt tcgccagatg gcaacatcca ggcagcggcg
     6481 tgagtacaac cagtcagttg tcccactggc tgaaaatcgt tgagatgacg taatgcatca
     6541 tccagtttgt tgagatcaaa cgtctgggtg aacggtagcg gctgcaccgg ttttccgatg
     6601 tcattaagtt gctccacgcc gcatacgcca cagcccgtac gtcccgccag cgcccggcgg
     6661 cgctccttca accccataaa gcggcggctg gaaagctcaa tttgtacttc aagaccatta
     6721 caggaaggaa cgacatccat gccgaagata tcgcgcggac tttctattat cccctcggaa
     6781 agcgaaaaac cgagcgcaaa gtactcaagg tctttgggcg acgccatcat caccacatgc
     6841 gaaatgccgt tgtagaccag cgcaacggga acttcttccg ccacctcatc taaccgtggg
     6901 tgttgtaaat catcacgccg ccataactca atttgacgaa cacctgtgac atttgtcaca
     6961 ttttcgattt ctttttgctg tgtttttttc actttctatt aaccggataa gaacacacac
     7021 accaacatta tggtattctg ttacaaaccc ttcctggatg gagggaaatt gagccaattc
     7081 tggacctttg cggccacttc cgcaaagaaa aataactccc actccctgca cacgcagcaa
     7141 gcgaatgtaa atgggacgtg acaatgtcga aacaaggagc aatccatgca ggtcagcaga
     7201 aggcagttct ttaagatctg cgctggcggt atggcaggca ccacggcagc ggcactgggt
     7261 tttgcaccca gcgtagcact cgcggaaacc cggcagtata aactgctgcg cacccgcgaa
     7321 acccgtaata cctgcaccta ttgttccgta ggctgtgggc tgttgatgta cagcctcggt
     7381 gacggagcaa aaaacgccaa agcatctatc ttccatatcg aaggcgatcc ggatcacccg
     7441 gtcaaccgcg gtgcactttg tccgaaaggc gctggcctgg tggatttcat ccactccgaa
     7501 agccgtctga agtttccgga ataccgtgcg ccaggttctg ataaatggca gcaaatcagt
     7561 tgggaagagg cgtttgatcg catcgccaaa ctgatgaaag aagaccgcga tgctaactac
     7621 attgcgcaaa acgccgaagg cgtgactgtt aaccgctggc tctccaccgg gatgctgtgt
     7681 gcttccgcgt cgagtaacga aaccggctat ttaacgcaaa aattctcccg cgcgctgggt
     7741 atgctcgcgg tcgacaacca ggcgcgtgtc tgacacggac caacggtagc aagtcttgct
     7801 ccaacatttg gtcgcggtgc gatgaccaac cactgggtcg acatcaagaa cgccaacctc
     7861 gtcgtggtga tgggcggtaa cgccgctgaa gctcacccgg tcgggttccg ctgggcgatg
     7921 gaagccaaaa ttcacaacgg cgctaagctg attgtgatcg atcctcgctt tacgcgtacg
     7981 gcggcggtag ctgactacta tgcccctatt cgttccggta ctgacattgc tttcctgtca
     8041 ggcgtattgc tgtacctgct gaacaatgaa aaattcaacc gcgaatacac tgaagcctat
     8101 accaacgcca gcctgatcgt gcgtgaggat tacggctttg aagatggcct gttcaccggc
     8161 tacgacgcgg aaaaacgcaa gtacgataaa tcctcctgga cttatgaact ggacgaaaac
     8221 ggcttcgcca aacgcgatac cacgctgcaa catccgcgct gcgtgtggaa cttgctgaaa
     8281 cagcacgttt cccgctacac gccagatgtg gttgaaaaca tctgtggtac gccaaaagac
     8341 gcgttcctga aagtctgcga atacatcgca gaaaccagtg ctcacgataa aactgcctcg
     8401 ttcctgtatg ccctcggctg gacgcaacac tccgttggtg cgcaaaacat tcgtacgatg
     8461 gcgatgatcc agttgctgct cggtaacatg gggatggctg gcggcggcgt taacgccctg
     8521 cgcggccact ccaatattca ggggctgacg gacctggggc tgctgtcgca gagcctgccg
     8581 ggttacatga cgctgccaag cgagaagcag accgatctgc aaacctacct taccgccaac
     8641 acgccaaaac cgctgctgga aggccaggta aactactggg gcaactaccc gaaattcttc
     8701 gtctctatga tgaaggcctt ctttggtgat aaagcgacgg cggaaaatag ctggggcttt
     8761 gactggttgc cgaagtggga taaaggctac gacgtcctgc aatacttcga gatgatgaaa
     8821 gagggcaagg tcaatggcta tatctgccag ggctttaacc ctgttgcctc attcccgaac
     8881 aaaaacaaag tgatcggttg tctgtcgaaa ctgaagttcc tcgtcaccat cgacccgctg
     8941 aacactgaaa cctctaactt ctggcagaac cacggtgagc tgaacgaagt tgactcgtcg
     9001 aagatccaga ccgaagtgtt ccgtctgcca tcgacctgct tcgcagaaga gaacggttca
     9061 atcgttaact caggtcgctg gttgcagtgg cactggaaag gtgcggacgc cccggggatt
     9121 gcgctgactg acggcgagat cctctccggt atcttcctgc gcttgcgcaa gatgtatgcc
     9181 gaacagggtg gcgcgaaccc ggaccaggtg ctgaacatga cctggaacta cgccattccg
     9241 catgagccga agtcggaaga agtagcgatg gagagcaacg gtaaggcgct ggccgatatt
     9301 accgatccgg caaccggtgc ggttatcgtc aagaaaggcc aacaacttag ctcgttcgcc
     9361 caattgcgcg atgacggtac aacctcctgt ggctgctgga ttttcgccgg tagctggacg
     9421 ccggaaggca accagatggc gcgtcgtgat aacgccgatc cgtctggcct cggtaacacg
     9481 ctgggctggg catgggcatg gccgcttaac cgccgcattc tgtataaccg cgcctccgca
     9541 gatccgcagg gtaacccgtg ggatccgaag cgtcagttgc tgaaatggga cggcactaag
     9601 tggaccggct gggatattcc ggactacagc gcagcgcctc cgggtagcgg cgtcgggccg
     9661 tttatcatgc agcaggaagg catggggcgt ctgtttgccc tcgataagat ggcggaaggt
     9721 ccgttcccgg aacactacga gccgtttgaa acgccgctgg gaactaaccc gctgcatcca
     9781 aacgttatct cgaacccggc tgcgcgaatc tttaaagacg acgccgaagc attgggtaaa
     9841 gccgataagt tcccgtatgt cggaaccacc tatcgtctga ccgagcattt ccactactgg
     9901 accaaacacg cgctgttgaa cgcgattttg caaccagaac agtttgtgga aatcggggag
     9961 tcgctggcga ataaacttgg cattgcccag ggcgataccg tgaaagtctc ctccaaccgt
    10021 ggctatatca aagccaaagc ggtggtgacc aaacgtattc gcacgctgaa agcgaacggc
    10081 aaagatatcg ataccatcgg tattccgatt cactggggtt atgaaggcgt agctaaaaaa
    10141 ggctttattg ccaatacgtt aacgccattc gttggtgatg caaacacgca gacgccggag
    10201 tttaagtcct tccttgtgaa tgtggaaaag gtgtaacgga gacgacatat ggcttatcaa
    10261 tcgcaagata tcattcgtcg ttccgcgact aacggtctga cccccgcgcc tcaggcgcgg
    10321 gacttccagg aagaagtggc gaaactcatc gacgtcacca cctgtatcgg ctgtaaagcc
    10381 tgtcaggtgg cgtgttcaga gtggaacgac attcgcgata ccgtcggcaa taacattggg
    10441 gtgtacgaca accccaatga tttaagcgcc aaatcgtgga cggtgatgcg cttctcggaa
    10501 gtggagcaga acgacaaact ggaatggctg atccgcaaag acggctgtat gcactgttcc
    10561 gatccaggct gtctgaaagc gtgcccggcg gaaggtgcaa ttattcagta tgccaacggt
    10621 atcgtcgact tccagtccga gcagtgcatt ggctgcggtt attgcattgc gggctgtccg
    10681 ttcgacattc cgcgcctcaa cccggaagac aaccgcgtct acaaatgtac gttgtgcgtt
    10741 gaccgcgtgg tggttgggca agaaccagcc tgcgtgaaga cctgcccaac gggcgcgatt
    10801 cacttcggta cgaaagagtc gatgaaaacg ctggcgagcg agcgcgtggc ggagctgaaa
    10861 acccgcggtt acgacaatgc gggcctgtac gatccggcag gcgtcggtgg tacacacgtt
    10921 atgtacgtgc tgcaccatgc tgacaagcca aatctgtatc atggcttgcc ggagaacccg
    10981 gaaatcagcg aaaccgtgaa attctggaaa ggcatctgga aaccgctcgc agctgttggc
    11041 tttgcggcta ccttcgcagc cagtatcttc cactacgtgg gtgtcggtcc gaaccgtgcg
    11101 gatgaggaag agaataatct gcacgaagag aaagacgagg agcgcaaatg aaacgacgtg
    11161 acaccatcgt gcgctacacc gcgccggaac gtatcaacca ctggatcacc gccttctgct
    11221 tcatcctggc ggcggtgagc gggctgggct ttttgttccc gtccttcaac tggttgatgc
    11281 aaatcatggg cacaccgcag ctggcgcgaa ttctgcaccc gtttgtcggc gtggttatgt
    11341 ttgcctcgtt catcatcatg tttttccgtt actggcatca caacctaatc aatcgggatg
    11401 atatcttttg ggcgaagaat attcgtaaga tcgtcgtcaa cgaggaagta ggtgacaccg
    11461 ggcgttataa cttcggtcag aaatgcgttt tctgggcggc gattattttc ctggttctgc
    11521 tgctggtgag tggcgtgatt atctggcgtc cttattttgc gcctgctttc tcaatcccgg
    11581 tgatccgatt cgcgttaatg ctgcattcat ttgccgcagt ggcgttaatt gtggttatca
    11641 tggtgcatat ctacgccgcc ctttgggtga aaggcacgat taccgccatg gtggaaggat
    11701 gggttaccag cgcctgggcg aagaaacatc acccgcgctg gtaccgtgag gtccgcaaga
    11761 caacggaaaa gaaagctgaa tgagtattcg cataatcccg caagatgagc tgggttcgag
    11821 cgagaaacgt acggcggata tgattccgcc gttattgttc cctcggctca agaatttata
    11881 caaccgccgc gccgagcgtc tgcgcgagct ggcagaaaat aatccgctgg gtgattacct
    11941 gcgctttgct gcgcttatcg cccacgccca ggaagtggtg ctgtacgacc atccgctgga
    12001 gatggatctg actgcacgca ttaaagaagc cagcgcacaa ggcaagcccc cgctggatat
    12061 tcacgttctg ccgcgtgata agcactggca aaagctgctg atggcgctaa ttgctgagct
    12121 gaaacctgaa atgagcggcc cggcgctggc agtgattgag aatctggaga aggcatcgac
    12181 tcaggagctg gaagatatgg ccagcgcact gtttgcctct gatttctcat ccgtcagtag
    12241 cgataaagcg ccgtttatct gggctgcact gtcgctctac tgggcgcaga tggccaatct
    12301 gatccccggc aaagcccgcg ctgaatacgg cgaacaacgt caatattgcc cggtatgtgg
    12361 ctctatgccg gtgtccagca tggtgcaaat tggcaccact cagggtctgc gttacctgca
    12421 ctgcaacctg tgtgaaactg aatggcacgt agtgcgcgta aaatgcagca actgtgagca
    12481 gagcggcaaa ctgcattact ggtcgctgga tgacgaacag gccgcgatta aagccgaaag
    12541 ctgcgatgac tgcggcactt acctgaaaat tctctatcag gaaaaagaac cgaaagttga
    12601 agccgtggca gatgacctcg cctctctggt actggacgca cgaatggagc aagaaggcta
    12661 tgcccgcagt tccatcaacc cgttcctgtt tccgggtgaa ggggagtaat ctctgacgtt
    12721 ttccgggtgg cgctggtcgc ccggattaca gctcataggg tgtgacgaca ccatctctcg
    12781 tattccgcgt taccacctca agctatgccg ccagtgcctg ttttgcctca atttacccac
    12841 ccccacatca ataaggttat gggtatgaga ctgatgataa gcgtaagatt gcctgatgcg
    12901 ccgcgcttat taggcctacc aataaactgc aatacattga atttatacga ctttgtaggc
    12961 cgggtaaggc gttcacgccg catccggcat gaacaacgca cacgttgtca gcaatctgat
    13021 tgatatcatt ttttcccatt accattcctc gcgcagctta cgctggtatt cgacaccatc
    13081 ttcctcacgc ccttgccata tgccaaaagc actggttctt actgtttgcg attgatttac
    13141 caagtattga tctaccgctt cccgtaatag ctccgtacga ggaagattac gctgcacctc
    13201 aagatcatca agttgcttaa tcacctcatt cgataaatcg agtataattc tgcccatatc
    13261 aatacctgcc agagagttca taaatatttg accaatatca tttgatatct atggagagaa
    13321 aagtaccctt gtcgaatctt taaagaaagc tcatttacgc atcatttttt atttttgcga
    13381 ggcgttttcc agatatgatg taacagttgc agttattata agttcaagta aaatagcctt
    13441 gtattaatac cagaataagg gcatctgttt tttatattca agaatgaaaa atttttgtca
    13501 ttccttatgc tccttacact gatcatcaac accaactacc tccccctttt gcctgcctcc
    13561 ctttgcttta cgcaccggga attgctgacg ttccagatat tgctctaccg cttcgcgtaa
    13621 aattgcagat cgtgaaacct tacgccagcc ccgaagcttg ttcagccgtt taattgcctc
    13681 ttctgataaa tgaagaaaaa ccgtattcat cgccattgcc accctcttac atatattact
    13741 cattaatgta tgtgcgaaaa ttacgccgga aaattaatta acgaaagcgg atttttgcat
    13801 gttgttttgt ttttgcgaga cgttttccag aaataataag agcgttgcag ttgtttcatt
    13861 tgttactcaa aaatggttta gttatcgccc aggatgagtt atgggtgcta tgaatatatt
    13921 atattcaggg atatttattt tttcattgga tctttatttg cataatctga atggtgtacc
    13981 cgaaaaatgt cagcatccta acagcaccag gatttaaggt gaaattaatc ttccatcaca
    14041 gaccggtcag tactgccacc aaccggtctt tacttgctca tcgttataat tttgttggta
    14101 acagttcggg aaacaaggaa ttccttccgg aacacccccg gcatcatccg atagcattga
    14161 cgtcaatcat cggctcgacg gtgagttttt ttaatcgcat catagggtgg caacgctcag
    14221 cacgatacgg gtcataacaa aactcctgag cagcggcact atgccgcttt cacaggagtt
    14281 ctgaatgctg gtgctttctg tatggcttcg ccatgcctct cgcggctaag cgcctgcaca
    14341 catctctctt tgtatttcat ttttgcaatg cattggccga attctgataa tcctaatgag
    14401 ttttactctt cagactccca tccctacggt atacgagtta gattcatgaa tgaaatcgac
    14461 atttttaaaa aacgaatctt gtaaaacatc cgtgttgata ttaccttgca acccaacatt
    14521 aaatttgtta gagagatgaa gaactcgaat atcaaaaagg tcttttaaca tcattacttc
    14581 ttcggctgtt aatgaatcgc aactaaaata tccctgttga tcaaaaaggt tttctatttc
    14641 tccatcatcg gcgaggcgtt tttcaaagag tgtttgcaac atcgagttgc caaggacaat
    14701 ggatatcgtg cgatctttcg cagatttaca ccccatcaca acggcaacat tcgtatcccc
    14761 ctcaggagtt tcatttatcg ctgtcgctaa aatggtggtc aaacacgcaa acggaactgt
    14821 atttctttcg tgatttaatt tacctaccag ttcattccat ttatcaaaag ccgggcgata
    14881 tttgttgcgc acagaagtat ccgactggtt aaacaattta gccatgcgat tttttatcat
    14941 ctcgatatcc ggcgaaccag aaagactttg cgcataatac ggtcctgatg atgttgcata
    15001 cattaaagcc gcgcccacac ctgtcgccat gatgggcgca gccacggttt tggcaagggc
    15061 gacgctgcga tggctatgcg cttcacaggt taaaggaaca tattccactg actgcggtaa
    15121 ttccttacat gcctccctca tcattgccgt atgctttaca aacaacggtt tttcactgcc
    15181 caaaaaaact ttctgcttaa ttgcactaag aaaacgtaaa tcaaaaatga ttgtttttgg
    15241 tttattattt tttggcacat ttcctattat cccgctgaga acctttcttg ctgcatggag
    15301 atttttttgc acagcacata acacaattga cgaagccttc tctactgcgt cattgatttt
    15361 aattttaaaa tccgcatgat atacgttgac gggcaataca agtatgtctg gcgctacatc
    15421 tttggctgag tatttattgg tgatcgacgg tcgtgaatcc acgagaaaac ttagctgcaa
    15481 attatgctct atattaccag ctgtttttgt ggtgtgatta aacatagaga tgccacctct
    15541 cattctttca cacatatcct caactttttc acttcgttct tgtggcaatt tggctttaac
    15601 gttaatttga tgatttttac ccgtgcattc cttaactaca aggggaatgt tatctaaagt
    15661 gccatacagg ctaataacgt caccaggatt tttatcatgt aagtaagttt gattaatact
    15721 cgaagcattc acaaagaggt caatattata actgtttata acttcaccta catcaaatgt
    15781 gtagtgctta ctctggaggc tgccatcctt atttaattgt atatcaatca atatatcccc
    15841 ggactttgtc accatatctt tgccataaac catttgcata gccatttcga ggagattttt
    15901 atctaactga ttgcgggcgt tgagtaagcg actaccaaat aatttatata ctgttcccgt
    15961 cattgccagg gctgcgcttt ctggtacttt gcgtttccac agtgaggttt ctctattttt
    16021 aaacaggtca tattcattat tactcgacaa accatatttc agttcgttaa cacaatggaa
    16081 cagttgttcc tcaccttcca ttttaaggcg atgtgaaaaa tcatttaata gcgtttgctt
    16141 cggtgattca ttactcatta gttgtttttc taaattaaca acagaataac tttcttgttg
    16201 aatgagatta atattggcaa ccattacagc ctcctttcca cagttaagag caaacataca
    16261 acataattat ttataacatc cagagcagta aatgaatgat actgacaatg atgccagccc
    16321 attatctttg cataatattt gttagctata gattttaaaa atatttatga gaaaagtatg
    16381 gcaccagagg gggttttgac tatcaaaaat cgctcaaaca tgaactaaga tgatgaatta
    16441 agcattatct gcgataattg ccatacagct ctacaaataa tatttaaatg ctcctgaaca
    16501 gtgatgtagc aagacacgta tggagccaac gaagaataaa aatttataga gttcttattc
    16561 aatataaccg aaatagccaa ggactaatat aaatgacctg aatgagagag gcagcaccgc
    16621 ccctctccat cagctactct tcttcgttcc cgccctcttc atacgggcca aatggcttcg
    16681 cgggcagaac gatatacgtg ccttcaaaca ctgcacccgg cgtctcgtcg ccaaagattt
    16741 cgacctgcat ctgcacccgt gcttttcgtc cgcgcgccag acggtcgaga tcgccgctta
    16801 aggcaccgag gtcggctacc gcatgaggtt taccgctaat cggcttgctg tagcggatat
    16861 gcgcatccgc aagaataatc gttccgccga ggtggcgttc acgcagcatc agccagataa
    16921 gtccccaacc ggtgagcgtc gccagtgaga ataaactccc ggcaaacagc gtatggtgcg
    16981 gattctgatt gccggtttct ggcatggtag tgataaattt ttgcccggta tattgctgaa
    17041 tgcgcacgcc cattttttca ctaagcggga tatgttcgta ccacgcctgt tgcagctgcg
    17101 cgcaccagtc gccgcgatgc agaatatcat ccagagtggc gacgggctta atcatcaaaa
    17161 aatggcgaat cggcgtggtg gttggcgtgg tgatttctcc ctgattaaca aaccccagct
    17221 tggcgaaaaa ctccaccgcg tcttcacggg cgctacaggt cacgcgctta acgccttcct
    17281 gacgcgccac cgactccagg gtcatcgcca tcagcgtgcc taaccctttg tcctgcacgt
    17341 cgggatgaac ggccataaag cgaatggacg cttcattgtc ggcattaata tacagtcggc
    17401 ctaccgccac cagattaccc tgctcgtcga cgaccatctg gtgatgcgcc atcgcatccc
    17461 acgcgtcgcg ttccgaacct tttggttgat gcaggggctt acgcaacatt tcccagcgaa
    17521 actgatagta acgctctaat tcttcttctg tttgtggaac ccgaaggtga tacatagcga
    17581 tactctctct tgtttcccgt gacaaccctg gaagctggct catacctgca accagaatgt
    17641 cacggggcca tcattgacca gcgatacctg catatccgca gcgaagcgtc ctgtttgcgt
    17701 gttcatctct tgctgacggc agcgttcgac gaaatagtca tataacgcct ctgcgcgatc
    17761 cggtgatgca cctttggaga aacttggacg catcccccgt tcggtatctg cggcgagggt
    17821 aaactgggaa accaccagca cactgccgcc cgcctgttgc acgttgaggt tcatcttgcc
    17881 ttcggcatcg ctaaagatgc gatagccgag cacacgctcg cacagacggt ttgctttctg
    17941 ttcgtcgtca tccttttcga cacccaataa caccaaaagt cccgcgccaa tttcgcccgt
    18001 cacttctccc tccacggtga cgctggcacg ggttacgcgt tgaattaatg caatcatggt
    18061 tcgtcgtctt cttcttgttc agctgcttgt tttagtttgc ggtattcccc gagagtgaca
    18121 gtaatttccg cgccaagcaa gacgatacac catgtccagt agacccaaac aaagagaatg
    18181 gggatcaccg ccagcacacc gtaaatgagc tgatatgacg ggaacatggt gatataaagc
    18241 gcgaaaccct tctttcctgc ttcgaacagg agcgcggcga caaacgcgcc gacaatcgcg
    18301 tcgcggttag gtacgcggat ggtaggaaca atgctgtaca gcaaccagaa ggagatccac
    18361 gacaacagca gcggaaaaat acgcagcaca ttgtcgatga cagtattgag atcgctcgcc
    18421 cagcgcaggg agagcaaata ggaactgatc gccagactgg cccctgccag cagcggcccc
    18481 agcgttaaaa tcatccagta cacggcgaac gagtaaattt ttggtcgagc tcgtttactg
    18541 cgccagatgg tattcaacgc gctatcgatg gagtacatca acaataacgc ggtgacgatc
    18601 agcccgcacg ccccaacggc ggtcatcttg ttggaattgg caacaaattg ttcgatatac
    18661 cgctgaataa catcgccagt agcaggcaga aagttggcaa aaataaagtg acgtaactga
    18721 atgctgacgt cggaaaacat gggaaaagcg gcgaaaagcg caaaaacaac ggcaaccagc
    18781 ggcactaatg agagcaacga cacataggca aggttacctg ccagggttgt catattgtcc
    18841 tcatcaatgc gttgccagag tagttttagc caggcccata gtggacgggt acggtgcctg
    18901 gctttgtcct gaatggtttt tagcataaca ccttcgcgaa atagtccggg atggtggttt
    18961 tatctttcac cagaatactg gtaatgccta gctgattagc tccttctata ttatcggcgt
    19021 tatcgtcgaa aaaggccgta tcgctgggtg aaaaaccttc cgcctgcaaa acatgctggt
    19081 aaattcgagc ttcaggtttg cgcatcccca gatcttgcga cagatagata tggtcagcag
    19141 catcacgaat ttccgggtat tcttccggcc agaaggtggt atgcaggcgg ttggtattgg
    19201 aaagcaccac cacgcgatgc ccctgctcac gcagtttatg catgatggcg atcacttccg
    19261 ggcgcagcgc aacaaacacc gcctgccagc cgtgagagaa ctgctcgtag cttagcggta
    19321 gagccatctc atgacacagc gcctctgcga acgcttcgtc gctaatttcc ccacgctcat
    19381 gctgatgaaa cgcctccccc atatgaaaac tcttcttaag cgtagccagc ggaatacgcg
    19441 ttaaatcgct ccaggctccc agcacacggt taaagtcgat atcgacaatc acattaccta
    19501 aatcaaagat atagagcatt tttgcctcct ttcgcgccac aagaaattaa ctgtagcggg
    19561 aaaggatggg tttggctatg tggcaataag ggtaatcttg tgatgctgat tgcgaaaaag
    19621 cagagtaatt tcactggctt tttcgcaata gatgaatggt taagattcag gtgtaaattg
    19681 ccaacgagtt tcgataacca gcggcctatc tggtgtcacc aattccggcg ttagatttat
    19741 tgcgttaggt gcctgagtca gcgggttaat acaggtggca tcgggctgtt tatcaaatac
    19801 caccagactg tttgcagaag agatcatggt catcgcgagt ttaccaggcc acagtaactt
    19861 aaccttcacc ccgtcataaa aaccaaaaca gtcatcccag ggaccgactt gaggagagat
    19921 gcgatttcca gttggcagtt catcactacc cgcttcttct tgccagtccg catcaaaaag
    19981 cacctgcaac gattctgtat tttgcggagt cagctttttc gcaaaccacg gatgccagcc
    20041 tgcggaggcc gggaatgtat cggcatatga atggacttcc agttgcaaaa ccagcgcatc
    20101 gttctccagc aaaaaagtct gaatcacttc cccttgccaa ggccacggtg aagccagggg
    20161 catacgtaaa gtcagggaat ctgctgtttt gtcgatgatt tcccacgttg agtaacaagc
    20221 cataccatgc aacgcatgtg gtggtttatt ggcgggtaaa gaataacatt gcccaccagc
    20281 attcagcgtc gcattcccca gtcgccctgc ccacggcacc attgggaaac agccatactg
    20341 aaaagctcta cgctgcggct gccattggcg taatacttca gaaccaaatg cctttaacga
    20401 ggttatcctg gcaccatcat gcggataaat ctgcaggtga atattgcctt tttccagcgt
    20461 cactaactct cctacaggcc caggccatgg cgcgtcaaac gcctgttgta gcgccaggta
    20521 atgctcgttt tttcgttcag ttttcatgag attgttccct gatgtagcgg taagaagcgg
    20581 tttcttgttc atcggtcggc tcaatcagcc agacgcaaac aaaggaaaga actgagacag
    20641 caaataaaaa cagagcgaca taccacggtt ttcctccccc tagcgccagc aaacttgagg
    20701 cgataagggg gcttagtccg ccaagaatcg cggaaaattg ataagcaaaa gaaaggccgg
    20761 tatatcgtac tttggtaccg aacatgcggg tgaaaagtgt tggctgcacg gcaaacatca
    20821 tcgtcggacc taagttgtag ccgaggatca tactgcacca gatgatgagc gtacttttgc
    20881 tatccagtaa taagaagaaa ggaaacgcga acaggacaca aaatcctgct cccgacaaat
    20941 acagactttt ttgacctata cgatctgata accatccaac caatggacaa ctgaaaatac
    21001 cgatcgccga ggcaatcaac atacccgtca ggggaatatc tcgcgataat gctaattggc
    21061 tggaaatata gacaatacca aaggcgttaa taatattaga ggagacactt tccgccagcc
    21121 tcgcacccag tgccaataaa atatttcgtg gatgtttttt gaataattca atcaacggag
    21181 gaacggactt ttctttatta ttcgtcgttt tttgcttttg aaaatccagc gtttcttcgg
    21241 tatgcaggcg gataaacagc ccaacgatca acatcaccgc agaaagccaa aacggaatgc
    21301 gccatcccca ggagagaaac tgctccgaag taagaaaatg gttacagaga gcaaaaatac
    21361 cggttgccag cataataccc acagacgcag ccgtttgcgg gagtgaacct aagaatccgc
    21421 gtcgggattc gggcgctgat tctatggtca tcaatgccgc tccaccgtac tcgccaccaa
    21481 gaccaaagcc ctgaatcaaa cgcagcacca ttaatacaag cggtgcccaa atgccaattt
    21541 cctgatacgt cggaataaaa ccaatcaaaa atgtggagca accgacaata gccagcgtcc
    21601 agataagtgt tatttttcgc ccgattttat cgccaaaatg accaaagacg atgcccccta
    21661 aaggacgagt cagaaaaccg acaccaaagg tcgcaaagga agctataaga cctataaaag
    21721 gatcgctgtc tggataaaac agcggtgcaa ataccagtcc tgctgccgtg ccaaaaataa
    21781 agaagtcata ccattccatt aacgcgcctg cgaaactgcc cataacgaca cgtctcatgg
    21841 ctttaccgga aagcccttca cgcggtgcgg gtaacttttc agacataatt ttcctctcct
    21901 ggtaggcgag aaatctggga tacgaaccac taaacgtggg cagtttgaat taatgcgcat
    21961 cggcttaagc cgatgcgcat taaattattt cttcaattca gccagaacct ctgcgtcctc
    22021 agcatcaaca tttgcaccca gtggttccca atgaatcaat acgcaggtca ggaaccccag
    22081 gaatggcacg attgccaacc agtaaaacgc ttcagttttc aacgcacccc acagcatcgg
    22141 ccagaaaatg agtcctgcaa ttgcccctgt gcgcataata gcgcgggcaa atccgacccc
    22201 agtagggcga atagcgggag gataagagag tgtggcaatg gtcatgccca gcccaccagg
    22261 acccgctgaa tggaagaaaa taattgatcc caataaccct aaagacagcc agggattagt
    22321 cgttgccagt gcgcctaacg acagcaatgc taaggtcaca acagcaaatc catacatcga
    22381 ctgtcggcga gtacccagtc gttggagaat aaacgagccg atcatcccgc ctgtcacccc
    22441 acaaagggta ttcacgacgg cagaaccggt caacgcgcca gtaagcccgc cacttattat
    22501 tcccgccaat gtaagaggaa gataaacacc tacggcgtta tattgccacg cctgcatggt
    22561 tgccacgaca cagccaagaa tggtgcgttt gcggtaacga tcattaaata agatgccata
    22621 tccaccactg tatttattct ctgctttttc cgagaatttc gcttctgttg tactcgatgc
    22681 cgcaacgcga gcctgaacac cataacgttt tcccagaatg tcgcacgctt cctggtaacg
    22741 cccgacgcgt gcggcccaca ttgcggattc accaataaag aagtaacgca aaatcatgaa
    22801 gatgactgca aaaatagctc cgatgaatat tccgtaacgc cacaattgtt cttcagcgat
    22861 acccgacaga agcagcggca aaagtaccaa ataaacaacg gttgtcgaaa cataccaggc
    22921 catttgccag aggttgaccg acgttccggt tttcttcgac gttgaaccac gaagctctgc
    22981 cacggcattg gttgccaaag ggaagtcgat gcctaaacca aagcccatga tgacgcggca
    23041 caccagcacc caccagatac tgggagcaat agctaccgct gcagcaccga tggtgcacag
    23101 ccccatgcca ataataaatg cccgcttttg cccaaagcgg tttattactc ggttcgctaa
    23161 taaaccgcca attaacgcgg cgattaatac tgatgcattg acggttgcag caagacccgg
    23221 cgaaatgccg aattgagcgg caatgtattt attaccaaac cccaccatcg ccgcctggta
    23281 ggcatcaatc aatatgccgc ccaaagcaat aaaaataacg ccgatactgg tgttgattgc
    23341 agagttctta ttaacaatat caattacgtc ctgtggccga ctaattgaat aagttgcagg
    23401 actggtgatt tgactcatcg cagtgtcctt tttaattgtt gtagggtggc gctgacgtga
    23461 cgcagcgcca gacagagatt tagaaaatca ttccggcgca aattgcagca tgacctttat
    23521 ggagtcattt tgctgcgcgc gttcgaatgc cgccgctgca tcctcaatgg caaataaatc
    23581 ggtaaccagt tcactggtat caaaccgccc atccgccagc cattcgataa cagcatcgaa
    23641 atcttcaggg atgtacatgc cagagttgag caagtcacgc tcgaaacgtt gcatcatcgg
    23701 taatggtatt tcacgcggcc cacttggtac gcccatgcac acgacggtgc ctccggcata
    23761 tacacgggta catgcatcgt taagcgtggc ctgcgctgca acgacatcaa tcgcccccgt
    23821 aaatcgcaca tctgcggcca gctcacctgg tgcccaaact tctgctgcgc ccagctttaa
    23881 cgccagcgcg cgtttggcag cgtcaggttc aatcacggta atcttacctg cccccataat
    23941 gcgcagtgcc tgcacgatgg ataagcctat cgttccggca ccaatcacca gtacgctttc
    24001 cagagaagct ttcggcattc ggttaacgca gtgacgggca caagccgccg gttcggcaaa
    24061 ggccaacact tttagcggta aagaggctgg tgcgacatgg caattacgcg caggaacaat
    24121 gtgttgtgag cgagcaaagc ccggtgcgcg aaaaccagca acctgcggtg gttcacaaag
    24181 attaaaacgt cctgctttgc aggctcggca ttccatgcaa gccatgatgg gatcgaccac
    24241 aacatgatcg cccggctgta catttttaac gtcgctgcca acttccgtaa cgcgcgctgc
    24301 aatttcatga ccggggacga caggtggttt ggcaaacgga tgaccgcctt tcagaacatg
    24361 cagatccgaa ccgcatatcc catagaacac aggcgcaatc cgcacttcgt gcggtttaag
    24421 ttcctggtgt ggtacttcaa catattgagt ggtgactttc ccaatatctg aaaataaaac
    24481 ctgggtaatt ttatccattg ttattctcca gaatcgatat ccatagggga ttagcgttgc
    24541 gcgatacctt catcgacaat ttctgccaac cgttgtaaac gaggaacaga gacatcgcgc
    24601 agcataattt gtggatcttg tgcaccaaca accgacgccc aaaccgctcg tccagccagg
    24661 aagcctgacg caccaccttt catggcaata cttactgcac ggccaaaaat atcggcatca
    24721 acgccagaag aaagaattac ccacggcatc ttcatctgat cgttaagttg ctgacaggcg
    24781 gcaagtaagg ttttttcatc gcctttgccc cctaatggca tctcggcttt gtaaagatca
    24841 gcctctgttc caccaagctc tgcggcggcg gcaacaatgg cgctttcacg atcaaagtcc
    24901 caaccgcgac gaggtggacg caccacaggt tcaataatgc taaccagccc ggcactacgg
    24961 cagcgacgaa caaatttatc aaccattgcc agacgttcct ctgcgggttc atcttcacgc
    25021 cagagtacaa gtaatttcat tgccttagcc cccatttcac gggctttatg cgggtcgacg
    25081 gacatatcta tttcaacgct atcaaccggg atgccattac ctggaataaa aagatcggca
    25141 gcgacaatca ggccgcaaga attagcaaca gcgccctgct cgactatttg ctcaagacaa
    25201 aattgtttat ccgccagaac ggcagaagca taaggagaaa ggattcgggt cgcggcaact
    25261 ttaaaatcgg tgagtacact gtcggcgatg ggttttggct gccctgctgc cgcaaacatc
    25321 aagcgcatgg cctcacgctg atcgacagct aacatggcaa atccaccacc tgggcgggag
    25381 atatctttca gcgtaaatgg ggtgaagtgg gatgacatag aaactcctgt taatagataa
    25441 aattaaagac cagcctgctg gcgaacctga tttagcaggg cactccagtc ctgccgccct
    25501 ttgccgtctt gacttgctgc agcgtaataa ctttctgctg caatccccat attgagcggt
    25561 gcccccgtct cccgagcgac gtcaactgca atacgcagat ctttcaacgc cagatccacc
    25621 atgaaggcgg gtgaaagatc gcctttcagc actttgccag gccaggtggt cgtaaaatgt
    25681 cctttccctg ctgccgtacc actcatcacc ttaattgcga catcaagatt taatcccagt
    25741 gactcacata acacagcggc ctcggaggac agcgcattaa gcgcaatgct catatagttg
    25801 ttaattagct tcacgcggat ccccattcca gggccgccag cctcgaccag ctcattaccc
    25861 atgcacatga gaatcgggcg tgcacgtgca atctgctcat cagtgccacc cgctaaaatc
    25921 agcaatgtgc catcaatcgc attcacggac gttctgccca cgggggcgtc catcatactg
    25981 atgccttttt cttgtaactc ccgtattaat gcatcggtct gtaagggatg aatagtcgac
    26041 atatcaatca ataacgcgtc agatgaaaca gcctcacaga cgcctttttc ccccaacaga
    26101 acctgtcgaa caatgtcacc attgggaagc atagtgatga taaattcagc gcctgctgct
    26161 gcttccgctg gggtttgtgc cgcagtggcc ccctgcgtaa ctagcgcatc gacggccagt
    26221 gcattgacat caaatacctg caatgaatgc cctttatgca atagattgtt agccattggg
    26281 ctacccatct gccccagccc gagaaatgca atggttgcca tgaatactct cctgtctgac
    26341 attgacaaaa taatgacaat tgaaaattcg ttttcgtctg tttttgagca tatttgtaat
    26401 ttatgttcaa aaacacgaca tacatcacta atttgatcat ttgtgtcatt taaattgacc
    26461 aaagacgaca aaattaaaca ggacaagaca aatatgacca gaattgcatg cgtgggtatc
    26521 acagttcagg acaggattta ttctttgccc actctgccgg agggcggcgg gaaatatcag
    26581 gcaaatcatt atcttgagat tggtggcggc ccggcagcaa cagccgctgt tgcaatcgca
    26641 aggcttggcg tggaagttga tttcatcggt cgcgtcggcg atgacagttg tggaaatacc
    26701 ttacttgcag aacttgaggg ttggggcgtc aatacagcat tttgccgccg atatcctaac
    26761 gcccgttcgt cgcaatcagc gatcctcgtt gatcagcatg gcgaacggat tatcgttaat
    26821 tatccaagtc ctgatctcgg tactgatgcc gaatggttag aagcgatcga tttctctcgt
    26881 tatgatctga ttctggccga tgttcgctgg catagcggta ctgaaaaagc attctcgcta
    26941 gcgcgccttg cgggtgtgac aacgctttta gacgccgata tgacaccgca ggatatttct
    27001 ccacttgtcg cgcttgccga tcacgccgtg ttctcaacgc cagggctgaa acgcatgact
    27061 ggcctgcagt cacctgaaga aggtttgttc caggcaacga cacaaacagc aggtaaagtg
    27121 tatgttacgc tgggttctga aggatcgttg tggattgaag atggtcattt gtgtcaacag
    27181 gaggcatttt ctgtcaatgt ggttgatacc actggagcag gcgatgtttt tcacggcgca
    27241 ctcgcggtag cgttggcaga aaaaatgcca acaaaagagg ctattcgttt tgccagtgcg
    27301 gttgccgcga tgaaatgtac ccagcctggc ggtcgggccg gcattcctaa tcgtgagcaa
    27361 accgaatcat ttttgtcact ttatgcgtaa aatggtaaac atctaccgag gaggggcaag
    27421 ggagtattca tgagtatcat cgaagtgaca ggtaatccac gccatgacca actcgttcat
    27481 ctaatcgcag aacgcggtta tatgaatatt gaagaactgg cacaactgct ggatgtttca
    27541 acgcaaaccg tgcgccggga tatccgaaaa ctcagtgagc agggcttgat cacccgtcat
    27601 catggagggg ccgggcgcgt ctccagcgtc atgaataccg cttttgagca acgggaactt
    27661 tcgctcaccg ccgagaaacg ggcgatcgct gaggcagtcg ccgattacct tcccgaacgc
    27721 tgtaccgtct ttatcaccat cggtacaacc gtagaagccg ttgccagggc attactcaac
    27781 cggcgtgatt tacgcattat cactaacagc ctacgtgtgg cacagattct ttataagaat
    27841 caggatattg aagtgatggt gccgggaggc actttacgcg ctcataacgg cgggattatc
    27901 ggcccaggag ccgtggattt tattgaaggc ttccgcgcag attatttaat caccagtata
    27961 ggtgccattg aacatgacgg caccctactg gaatttgatg taaatgaagc gttagtcgcg
    28021 agaacgatga ttaaacatgc gcggaataca ttgttagtgg ccgatcatac gaagtttgcc
    28081 gcgtctgctg ccgtttcaat tggcaatgca cggaatgtca gggctttttt tactgatgcc
    28141 ccgcctccca attctttctg ccagttgtta agtgaagaga atgttgaact ggtggttgcc
    28201 gagcaggaag tttcctgaaa ttgaaacttc gaaaaatccg ctggggattc agcggatttt
    28261 attttgccgg atgcggccta cggttgatac ggttttcgtt ggccggatta agcacatccg
    28321 gccattaatt aagcttcagc cgccagcatc tctttcttac gttggctaat acgcttaacc
    28381 agcattacgg aaaccaccgc accacagaat gcgaagcagg ccataatgcc aaacacgatt
    28441 ttgtagccaa taatccccgg atttaaatcc agaatgtagc catacagact gaagcagaac
    28501 atcgccgggg cgtaaccaat aaagctaccc aacgccatcg ccgcgcctgt tttattttca
    28561 gcaatttttg cttcgccgat aggtgcaaaa aatacggctc gctgtgtaaa gactatcgcg
    28621 ccaaagccca gcgtacatgc catccctaaa tagaccggca tactttcgtg aggtagcata
    28681 atcaacagta cgagcgcagc ggtactgatg ataaaggtgt agcatagata tttactcggc
    28741 gatttcagga tcttatctga aatcatgcca ccaatcggtc caccaatcat tttcagacag
    28801 tattggttaa tgatgccgta agcccccacc agcgcaacgg gcaatagata gatgtttttc
    28861 aggaatggaa tgaagaatgt caggccacag taaaccgcgt aaacgaagaa gacgttgaaa
    28921 gcgataagcc agatagtttt gtctttcagc accgaggtca tactggtgtt ttgcgatgcc
    28981 ccgtcttctt ttttcaactt aacggacggt gcctcttctt tgtcattcag gacaaagaaa
    29041 ataataatac ctacggcaat cacaataagg gaatagaacc agatgcctgc tttaaaaccc
    29101 aataagccac tgccaaacca ggtaaatact gccaacgcag aaaatgccac cacggtatcg
    29161 acaatgccac gccctgtttc gaagaagcca aacaaccgac cttgttgttc gctattgccc
    29221 aatcgactta ccgacttgag caagaccggc cagttcatca tgtcgcaagt aacgccaaat
    29281 agcgcccaga caaagaggat gccccaatac cccggcattg tcgtcaaata aacacccagt
    29341 aatcctgtcg caatgagtga gaatgacatg gtgtatctgc gcggtagttt atcagcaaaa
    29401 taaatagaca gaaaaaagcc cactgtggta acaaatgagt ttaccgacat agcattacca
    29461 atttgaccat tggtcaaatg gaaatattcc tgcatcggga tataaaacgc atctttcagc
    29521 gacggtaatt tataaattgt accgccacac agtgttaata gactaaataa cgcccatttc
    29581 tttttcgtga gcataacatc ccctaagcga tttttaaaat cactgtttta aaggcaataa
    29641 gataccgacc attcatatgg aatggtttta ttaactgcgt atgagttaaa acttattagt
    29701 tgttcagaag atggtttgcc tcttgtttga tttgttgtag tacgttacgc acatgattta
    29761 tctgattaat tgcatcttct ttttctttac gtaaccgcgc atccagtaaa tgattttctg
    29821 gtaggggtgt ttcactcatc tggcgataag ggatccacgg attatcatct tcgtcttcaa
    29881 agcggaacgc cggcgcatag taatcaacct cttcttccag gccatatgcc gtcacctgcg
    29941 gctcatcatc acccaggcag ataaggtgcg ttaataacgc tttgaacggc atatcaccct
    30001 gacctgaaat acaggcttta tgacccaggc caccctgttc tttaacgatc aaggcatctt
    30061 taatatggac ctgggtaata tgcggtgcca tcgtttttaa ggcgtcgatg ggatgctcat
    30121 ttgcattgat catgttcgca aaatcaaaca gtaaggaaag agactccatc tcactttctt
    30181 tgaccagcga caccaactca tgacttttta aatcttcatg ctgctcaatc gtaaaagtca
    30241 ggccgctgtc ctgatacgtt tctcgtacat aggcaatgtc gttagcgata atcgataata
    30301 cgtcgcgcaa attaccttca taacgcggat aaaaacgtac ggacgatgcc ccagttttca
    30361 acgcgatggc gacggcttcg tcaatagatt ttttatctga ggcgctggtt tcaatatgaa
    30421 tatcaaggtt cagacggcgg gctttatccc caaaggcaga gagttcttta tcgtccatat
    30481 taccaagaga aaaacgctcg ccatccagaa catggatttt gacgccacgt agattatttt
    30541 cacttgcgat atcaagtaaa tcagcaggca acactctttc cagccgcata tttaaatgaa
    30601 aggcataagc atgtaaataa agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt
    30661 tatttattgt catcataaaa cacctacaga ttaatgatga ggtctacggc ctcaaatcgc
    30721 caatattcaa tatctatctg gacaatattg ccttgctggt cggcgcgatg cttttcaatt
    30781 aagatcgctg gcatacccgc agaaccgcca agatacttgc tgatatcgcc cggcattctg
    30841 acgggtttga aaatcaattt ttttactacc gtctcttttt ggtagcactt ttcccagact
    30901 gcagaaaatg agtggttttc aagttgttca ataaaacccg gagcaacttc aggattaata
    30961 tatgtttcgt gatagaaaac tttatgtcct tccagcgccc cccagccggt gatgcgatag
    31021 agttcagttg atggtgtcac tgcaatcagt ggcgcgagcg tctcgggaat atcgctggta
    31081 cggcttttct cggtaaaccc ccaggaaggt tcccgccctt gctcaattgc ggcccgctga
    31141 aagctcgccg acagctccgg actgtaatta aatcgcggct gggtcacgaa ccacccctta
    31201 cgatccttac ggaagatttt ggattctgcc tcgaggttca acaacgcctg gcgcagcgtc
    31261 atacgtttaa tgcccagcag ttctcccagc tctctttctg aagggagttt tccacccggc
    31321 gtggtgatgc catctttcaa ccaccgatcc agtttctctt ttgcattttc tacggtggat
    31381 tgattctccg ccattgttta atccttttgg tttaaaccag atgaagcatc ttacgcttac
    31441 gccaaaaaag aaagcaatta acggcatatg actttgcaga taattgtgac aaagctctct
    31501 attgacgtaa ccacagactt ataagggaga tactcgcatt ggtgtgcgct cacgacaggc
    31561 aaaaaaaaac ctgccatcgc tggcaggttt tttataacta aaaaacgaaa ttaatcgtct
    31621 ttcggtgcgc ggttggcgcg acgacgatcg ttttccgtca ggtgacgttt acgaatacgg
    31681 atagaggtcg gagtcacttc taccagttcg tcatcatcga tgaactccag agcttgttcc
    31741 agagtcatgc ggataggcgg aaccagaaca acggcttcgt cagtaccgga agcacgcatg
    31801 ttggtcagtt tcttaccggt caggcagttt acagtcaggt cgttagagcg gctatgaata
    31861 ccgataatct gaccttcgta aacttctgca ccgtgaccga ggaacagctt accgcgatcc
    31921 tgcagaccga acagcgcgaa cgcgaccgct ttaccctgac cgttagagat cagtacaccg
    31981 ttctgacgct gacccacttc gcccggacgt acgtcgtcgt agtggctgaa ggtggagtac
    32041 agcagacccg taccggaagt catggtcatg aactcagaac ggaagccaat cagaccacgg
    32101 cttggaatca cgtagtcgag acgtacgcgg cctttaccgt ctggattcat gtttttcagg
    32161 tcgcctttac gttcgcccag cgcctgcatt acagaaccct gatgctgttc ttcaacgtcc
    32221 agagtcacgt tttcatacgg ctcttgttta cgaccgtcga tttcacggaa gataactttc
    32281 ggacgggata ccgccagttc gaaaccttca cgacgcatgt tttcgatcag aacagacagg
    32341 tgtagttcgc cacgaccaga aacgcggaac gcatcagcgt cttcggtttc ttctacgcgc
    32401 agcgcaacgt tgtgtaccag ttctttgttc agacgatcca ggatctgacg agacgttacg
    32461 aacttacctt ctttaccgca gaacggcgag gtgttaacgc agaagaacat agaaacggtc
    32521 ggctcatcaa cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca
    32581 gaaatgttca gttcgcccag gcccgtgatc gccacgatat cgccagcttc cgccagatcg
    32641 gtttcgatac gttccagacc gaggtggccc agcactttac cgactttcgc gttgcgggtt
    32701 ttgccttcgc tatcgatgat agtgacctgc tggttcggct tcactttacc gcgcttgatg
    32761 cggccaatgc cgataacgcc aacatagctg ttgtaatcga gctgagaaat ctgcatctgg
    32821 aacggaccgt caaggtcaac gtccggcgca ggaacgtggt caacaatcgc ctgatacagc
    32881 ggggtcatgt cttccgccat atcttcgtgg tccagacccg cgataccgtt cagcgcagaa
    32941 gcgtaaacga tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg
    33001 aatacctgat ccacaaccca atcaggacgc gcgccagggc ggtcaacttt gttgataaca
    33061 acaatcggct tcaggccgta agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc
    33121 gggccgtcaa atgcgtcaac caccagcagc actgagtcta ccatggacat tacacgttca
    33181 acttcaccac cgaagtcggc gtgccccggg gtatcaacga tgttgatacg gtaatcattc
    33241 catttgatag cggtgttttt cgcgaggatg gtaatcccac gctctttctc caaatcgttg
    33301 gagtccatca cgcgctcttg ggtttcggca cgagagtcga acgtaccgga ttgttggagc
    33361 agcttgtcta ccagggtggt tttaccatgg tctacgtgcg cgatgatggc gatattacgc
    33421 aatttttcga tcacaacttt gcctcaggca ttagaaatag cgcgttattg tacacggatt
    33481 aatcgcacta caaaacagga tcacaaacat cctccgcaaa caagtattgc agagtccctt
    33541 tgtgatcgct ttcacggagc ataaaaaggg ttatccaaag gtcattgcac caatatggtg
    33601 cttaatgttt ccattgaagc actatattgg tgcaacattc acatcatggt gcagcccttt
    33661 tgcacgatgt taagcatgat aacgcctttt aggggcaatt taaaagttgg cacagttttc
    33721 gctttatctt ttttacggcg acacggccaa aataattgca gatttcgtta ccacgacgac
    33781 aatgaccaat ccaggagagt taaagtatgt ccgctgaaca cgtactgacg atgctgaacg
    33841 agcacgaagt gaagtttgtt gatttgcgct tcaccgatac caaaggtaaa gaacagcacg
    33901 tcactatccc tgctcatcag gtgaatgctg aattcttcga agaaggcaaa atgtttgacg
    33961 gctcctcgat tggcggctgg aaaggcatta acgaatccga catggtgctg atgccagacg
    34021 catccaccgc agtgattgac ccgttcttcg ccgattctac cctgattatc cgttgcgaca
    34081 tccttgaacc tggcaccctg caaggctatg accgtgaccc gcgctccatc gcgaagcgcg
    34141 ctgaagatta cctgcgttcc actggcattg ccgacaccgt actgttcgga ccagaaccag
    34201 aatttttcct gttcgatgac atccgtttcg gatcatctat ctccggttcc cacgttgcta
    34261 tcgacgatat cgaaggcgca tggaactcct ccacccaata tgaaggtggt aacaaaggtc
    34321 accgtccagc agtgaaaggc ggttacttcc cggttccgcc ggtagactcg gctcaggata
    34381 ttcgttctga aatgtgtctg gtgatggaac agatgggtct ggtggttgaa gcccatcacc
    34441 acgaagtagc gactgctggt cagaacgaag tggctacccg cttcaatacc atgaccaaaa
    34501 aagctgacga aattcagatc tacaaatatg ttgtgcacaa cgtagcgcac cgcttcggta
    34561 aaaccgcgac ctttatgcca aaaccgatgt tcggtgataa cggctccggt atgcactgcc
    34621 acatgtcgct gtctaaaaac ggcgttaacc tgttcgcggg cgacaaatac gcaggtctgt
    34681 ctgagcaggc gctgtactac attggcggcg taatcaaaca cgctaaagcg attaatgccc
    34741 tggcaaaccc gaccaccaac tcttataagc gtctggtccc gggctacgaa gcaccggtaa
    34801 tgctggctta ctctgcacgc aaccgttctg cgtctatccg tattccggtg gtttcttctc
    34861 cgaaagcacg tcgtatcgaa gtacgtttcc cggatccggc ggctaacccg tacctgtgct
    34921 ttgctgccct gctgatggcc ggtcttgatg gtatcaagaa caagatccat ccgggcgaag
    34981 ctatggacaa aaacctgtat gacctgccgc cagaagaagc gaaagagatc ccacaggttg
    35041 caggctctct ggaagaagca ctgaacgaac tggatctgga ccgcgagttc ctgaaagccg
    35101 gtggcgtgtt cacggatgaa gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg
    35161 accgcgtgcg tatgactccg cacccggtag agtttgagct gtactacagc gtctaatagt
    35221 tgaagttgta ctacccggcg caacaacgcc gggatttagt tgccgtggaa actttcagcc
    35281 catctctgca tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta
    35341 aaaagctata atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg
    35401 cacgcagccc gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaatcga
    35461 tgacaacctg gcgatccatt acgccaaccc cgctgcgcaa caactgctcg cccaaagctc
    35521 ccgcaaattg tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga
    35581 gctgatgcaa gaaagcctgg aggcggggca aggttttacc gataacgaag tgacgctggt
    35641 gatcgacggg cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat
    35701 cctgctggag atggcgccga tggataacca gcgccgctta agtcaggaac agctacagca
    35761 cgcccagcag gttgctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc
    35821 gcttggcggt ttacgtggcg cggcgcagct tctcagcaaa gcattacctg acccgtcact
    35881 gctggaatat accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatc tggtcgaccg
    35941 tctgttgggg ccgcaactgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga
    36001 acgcgtggtg acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta
    36061 cgaccccagc ctgccagaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa
    36121 tattgtgcgc aatgcgctac aggcgctggg gccggagggt ggagaaatca ttctgcgtac
    36181 ccgcaccgcg tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga
    36241 tgtggaagat aacgggccag gcattccgcc tcatttgcag gatacgctgt tttacccgat
    36301 ggtcagcggc cgcgaaggtg gcaccgggct tggcttatcc atcgctcgta atttgattga
    36361 tcagcattca ggcaaaattg aatttaccag ttggccaggg cataccgagt tctcggttta
    36421 cctgcctatc aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat
    36481 gacgatagtt ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt
    36541 acgacatttg agaacggcgc ggaggtactg gaggcgctgg cgagcaaaac gccggatgtg
    36601 ctgctttcag atatccgtat gccgggaatg gacgggctgg cactgctcaa gcagattaaa
    36661 cagcgccatc cgatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc
    36721 gtcagcgcct atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa
    36781 gccgtggcgc tggttgagcg cgccatcagt cattaccagg aacagcagca gccgcgtaat
    36841 gttcagctta acggcccaac gaccgatatc atcggcgaag cgccagccat gcaggacgtg
    36901 ttccgtatta tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc
    36961 ggcaccggta aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg agccaaagcg
    37021 ccgtttatcg cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt
    37081 ggtcacgaga aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag
    37141 gctgatggcg gtacattatt cctcgatgaa attggcgata tgccgctgga tgtgcagacg
    37201 cgtttgctgc gcgtgctggc agacggtcag ttttaccgcg ttggcggcta tgcgccggtg
    37261 aaagtggatg tgcggattat cgctgccact caccagaatc tcgaacagcg ggtgcaggaa
    37321 ggtaagttcc gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg
    37381 ctgcgtgagc gtcgggaaga tattccccgc ctggcgcgcc attttttaca ggttgccgcg
    37441 cgagaactgg gcgtagaagc gaagttgctg catccggaaa ccgaagccgc gttgacgcgt
    37501 ctggcgtggc caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtaatg
    37561 gctgccggac aggaagtgtt gattcaggat ttgcctggcg aactgtttga atcaacggtt
    37621 gcggagagta cttcgcaaat gcaaccggac agttgggcga cgcttcttgc gcagtgggca
    37681 gacagagcgc tacgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag
    37741 cggacgttac tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg
    37801 ctactcggct ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga
    37861 ttcacggctt gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta
    37921 ctgttccgat aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa
    37981 tcaataatta atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt
    38041 gctgccgtgc tgtgtgccgc gatgattggg ctgtttagtt gagtttcaga ttgctgatga
    38101 agtgctcttt gttcatgccg gatgcggcgt gaacgcctta tccggtctac aaaagcaata
    38161 ttttcaacag gttttgtagg cctgataagc gtagcgcatc aggcaatctt gttctcagca
    38221 cattgatggc gctttcgttt tctactttgt aaacgaagcg ccattcacta tttaaatcac
    38281 ccgagagaac tgctgcatcc gcgctttctg gcgcagatag gtatcaaagc acatgcaaat
    38341 gttgcggatc agcaagcgac ctttcgccgt cacctgtatt cccttctcat ccacatccac
    38401 cagcccatct tttgctaacg gggcgagcag cttgagatct tccgcaaagt aatcagcgaa
    38461 gtgcaaatcc cactgtttct caataggggc gtaatccaga cggaagttgc agatgagcga
    38521 cttaatcaca tcgcggcgaa tacagtcatc acgcgttagc gcaataccac gccacagcgc
    38581 attgccttgt tcatccactt gctgatagta ctgtttcaac tctttctggt tctgcgcgta
    38641 gcagtcgcca atcatgctga tggcggaaac gcccatcccc agcagatcgg tatcgccctg
    38701 agtggtgtag ccctggaaat tacgatgcag cacgccttca cgctgggcca ccgccagctc
    38761 gtcatccgga cgggcaaagt gatccatacc gataaactga tagcccgatt gcgtcaggaa
    38821 ggcgatggtt tcctgcagga tatcgagttt ttgctgcgga ctcggcaggt cagcatcttt
    38881 gattttgcgc tgagcagcaa aaatggtcgg cagatgcgca tagttaaaga cgctcagacg
    38941 gtcagggttt agctccgcca cgcgtttcag ggtaaaggcg aaactttccg gcgtctgttt
    39001 cggcaggccg taaatcaggt cgatgttggt ggaggtaaag ccaatctcac gcgcatggtt
    39061 aagcagcgca aagataaact cttcatcctg ctcgcggtta accagacgtt gcacttcttt
    39121 gttgaagtcc tgcacgccca tactcaggcg attaaagccc tcggcgcgta aatgatcgag
    39181 tacatccagt tcgatttccc gcggatcgac ttcgatcgaa atctccgcat cggcattgaa
    39241 ctggaagttt tcgcgcagca gcttcatcag gcggctgatt tgcgctttat taagatacgt
    39301 cggcgttccg ccgccccagt gcagctggct gacgtgacgc ccggcgaaca gcggtgcacg
    39361 atggacgatt tcttgctcca gcgcgtccag atactgatcg gccttgtgct gctggcgagt
    39421 aacaatctta ttgcaaccgc agaagtaaca aagcttatgg cagaacggga tatgtacgta
    39481 gagagataat ggacgctcag gatagcgcgc cacggcttgt aaaaacgcct gttcgccgaa
    39541 gtcttctgaa aactccagcg cggtcgggta cgaggtgtat cgtggcccgg aatagttata
    39601 tttctggatc agggccagat cccagtcgat ttgctgtaca gacatgctca ctccttccga
    39661 tggcgtctct ggcggctacg gcgtaccggc ggatgcccgt ttcgggtcat cagccggttg
    39721 cgcagccact tttgtcgccg cgacaaccgc tgtagtttaa cgaataacca caccagataa
    39781 catataaccg gaagggttat cagcagaacg ggaaggccca cggcggcaat ccgttagttg
    39841 ccccgcagca gacgcatcat gtcttcttgc ttctcgtctt cttcctcttc ttcgtcatcg
    39901 tcataagaga ggccgagttt ctgcatcaac tcatcaatac gatccagttt agcatccacc
    39961 caggattgct cttcggcact cagggtttcg cctgcttcca gacgttccag cagcgcatcc
    40021 agacgctcat ccgtttccag taattccaac tccgcctgcg gtgaaagcat aggtttctca
    40081 ctcttcggtt tgtgctgttt ggtgactttt tcagtcacac ccaatggaat aggggtttta
    40141 ctgccaatac gtggatcttt cggtgcgttc tggcctttgc tgcctgacgt gttgtttccg
    40201 cccgctgcgc ggctgcccgg cgcatgacca cgacgttttt tctgacgctt gcggtcacga
    40261 gcttcctgat tcagctcttc gcgcgttttg cgacgcgctt ttgcatgacc tttgctgcgt
    40321 gatggtgaag atggtttcat aatggttcgt cttttagccg ttttattcag tatagatttg
    40381 cggcggaatc taacagaaag caagcaaaga aaaaaggcga cagattactc tgtcgccttt
    40441 tttcctgact cataaccctt aacgggtctt acaatccttg caggccgcca acacaccctg
    40501 tttttccatt agccggaaca atccgtgttg attccatttc cgtttaatta cgtctccgga
    40561 cgtttgtctt cctgacaatc ctctgtcttc gcctcctggc gctcctgacc ctcatcctga
    40621 gtcattgttc ctgttagcgt cctcgctcta tgggtgttac tttacgcctt tgctttaaac
    40681 aaacaatcaa ccgccatcag tatttgcaca ttttcagagt tttatataaa agcaattcac
    40741 tgatttatat agcttatttg acatgcatcg cagaattttc tctgctattt cgcataaaga
    40801 ctatcggcaa tgtctcacaa agcgtatggc ttttacttac aacgctcacg ccgaaaaatc
    40861 cgccttttat tatggttcag gtataatcgc cacaaagctt tgactgacgg agacgaccgc
    40921 tttgactaat ttgaattatc aacagacgca ttttgtgatg agtgcgcctg atattcgcca
    40981 cctaccttcc gataccggaa ttgaagtggc ttttgcaggc cgttccaacg caggtaaatc
    41041 cagcgcgctg aacacgctga ctaaccagaa aagcctggct cgtacctcaa aaaccccagg
    41101 acgcacccag cttatcaacc tgtttgaagt ggctgacggc aagcgtctgg ttgacttgcc
    41161 tgggtacggt tatgcggaag tcccggaaga gatgaagcgc aaatggcagc gcgcgctcgg
    41221 cgagtacctc gaaaaacgtc agagcctaca aggtctggta gtgctaatgg atattcgcca
    41281 tccgctgaaa gatttggatc agcagatgat tgagtgggcg gtagacagca atatcgccgt
    41341 gctggtactg ctgaccaaag cggacaaact ggcaagcggc gcacgtaaag cgcaattgaa
    41401 tatggtgcgt gaagcggtgc tggcgtttaa cggtgatgtg caggttgaaa cgttttcttc
    41461 gctgaagaaa caaggcgtgg acaagctgcg gcagaaactg gatacctggt ttaacgagat
    41521 gcagcctgta gaagaaacgc aggacggcga ataattttct taccttaatg cttatgccgg
    41581 atgtggcgtg tccggcccat aaattcaata gattacaatt ctaccgttgg cctgataaac
    41641 atggtgcatc aggcattgcg gattttttct ttcgcccaat aaaaaacgcc ccagtcatta
    41701 ctgactgggg cggctaaaat attcagccaa atccgattac gtgaagtaaa aggtctgaaa
    41761 gatagaacat cttacctctg taccctacgc gactaacttt actctttttt gttcagttca
    41821 gaaagcactt tttgtaattt tttttcagtt gttgcatagg aatttctaat agccatcaca
    41881 aaacgtgaca gcttatgttg cttacttacg aaaaaagggc atgttcaggc gaatcttagt
    41941 gcgcctgatc ccagttttcg ccactcccca cttccaccag caacggcaca tccagacggg
    42001 tacagttttc catcagttga tgaatctgct tcgcgacggc atcgacatca tctctatgaa
    42061 cttcaaatac cagttcatcg tgtacctgca tgatcatacg tacacgcggt tgctcagcct
    42121 gtaaccacgc atcaacggca atcatcgccc gtttgataat gtcggcggcg gttccctgca
    42181 ttggcgcgtt aatggctgca cgttcagccg ctgcacgacg agcaccattg ctggatttga
    42241 tatccggcag atacagacgg cgtccgtcca gcgtttcaac gtagccctgc tctttcgcct
    42301 gagcacgggt gcgttccata tacttcagca cgccagggta gcgttcgaag taaaggtcca
    42361 tgtacttctg cgcttcttta cgtggaatgt tcaattgccg cgccagaccg aaagcactca
    42421 tgccataaat cagaccaaag ttgatcgctt tcgcgctacg gcgttgctcg ctggtgacgg
    42481 tttccagtgg caaaccaaac acttctgccg ccgttgcccg gtggatatct tttccttccg
    42541 cgaatgcggt cagcaagcct ttgtcacgcg agagatgcgc cataatgcgc agttcaatct
    42601 gcgagtagtc cgctgagaca atcacataat cctctggcgc aataaacgcc tggcggatac
    42661 ggcgaccttc ttcgttacgt accggaatgt tttgcaggtt aggatcggtt gacgataaac
    42721 gtcccgttgc agttactgcc tggtgataag aggtatgcac acgcccggtt ttcgggttga
    42781 tcatcagcgg cagcttgtcg gtgtaggtcg atttcagctt cgccagacca cgatactcca
    42841 gaatcacttt tggcaacgga tagtccagcg ccagttcttc cagtacctct tccgacgttg
    42901 acggcgcgcc acccggcgtt ttcttcagcg gtttaatgcc ctgtttttca aacagaatgg
    42961 tttgtaactg cttggtggaa gaaaggttaa attcctcacc tgcaatttca tacgctttct
    43021 tttccagctc agccagacga agggtgagct cttcagaatg attgtgcagc actttcggat
    43081 cgatcttcac accattacgt tcaatgcgtg aaagcaccgg caccagcggc atttcgatat
    43141 tctcgaagac gttcaacggc cctttgtgtt tttgcagatc cggccacatt ttcagatgca
    43201 actgcaaggt gacatctgca tcttcggcgg cgtaacgtcc ggcctcatcg agggcaatct
    43261 ggttaaaggt cagttgattt ttgcctttac ccgcaatctc ttcaaaagtg atggttttgt
    43321 gcttcaacca acgttccgcg aggctgtcca tatcgtgacg cccggcaacg ctattgagaa
    43381 tgtaggactc cagcatggta tcaaacgcaa tcccacgcag ctcaatgccg taattcgcca
    43441 gaataccgcg atcgtatttc aggttttgcc cgaccttcag cgccttttca tcttccagca
    43501 gcggttttag caactcgagt gcacgctcgc gagagatttg atcgggcgca tcaagataat
    43561 catgagcaac cggaatatat gccgctacgc ctgactcgat agcaaaagaa agcccgacca
    43621 ggttagcaga gatgttatca aggctgtcgg tttcggtatc aaatgcaaat accggcgctt
    43681 tttccagctt cgcaatccac gctttcagtg tttcttcatc aaggatggtg acgtagttgt
    43741 cataagaaat caccgttgcc gtcacttctg gtgcttcgtc tgcaacactg gtttcctgtg
    43801 gcttcgcggc gggttttgcc cctttggcct gtaaccattt gcccgcttcg acatcagcag
    43861 tccagcgttt gaactcatac tttttgaaca gccccaacaa ctcttccgct gccggttgct
    43921 gcacttccag ttgttcacag gtcagctcca gttcaacgtc ggttttaatc gtcgccagct
    43981 ggtatgagag ataagcaact tctttgtttt gctcgagctt cgctgccatt gttttcgcgc
    44041 cacggaagct caacccagca attttttctg gctcggcata cagcgtatcc agcccgccaa
    44101 gaccttgcag caatgcctgc gcggtttttt caccgacgcc cggtacgcca ggaatgttat
    44161 cagaggagtc acccatcagc gccaggaaat cgatgatcag ttctggcggc acgccgtact
    44221 tattcaccac ctcttccggt ccgaggatgg tattcgtcat ggtattgata agcgtaatat
    44281 ttggcgtcac cagctgcgcc atatctttat cgccagtgct gatcagcacc ggacgcccgg
    44341 ctttttcggc ttcgcgcgcc agagtaccga taacgtcgtc cgcttctacg ccagaaaccg
    44401 ccagcagcgg cagtcccatc gctttaacca tcgcgtgcaa gggttcgatt tgtgctcgca
    44461 gatcgtccgg cattggcggg cgatgtgatt tgtaatgttc aaacagttca tcacgaaagg
    44521 tttttccctt ggcgtcaaag accaccgctg catgcgtcgg tttatattgc atgatcagac
    44581 tgcgcagcat gttgaggaca ccatacatcg caccggtcgg ttcgcctgcg ctgttagtca
    44641 gcggaggaaa cgcgtgatat gcgcgataaa gataagatga accatctaca aggataagtg
    44701 gattttgggg gatctgaacc ataatgtccg tgcctgttta tcagattatg ggtaaaggat
    44761 gccacagaca ggataaaaac atgagttttt caccgctttt cgtgaaaatc ttttgtagat
    44821 cttctggatc gctcgcaaat cgtcatgtgg ataactttgt gcataaatta tttacatcgc
    44881 ttttaattta ttatcatgct ttttacagta tgatgtttgt ctttgttttt catgcagtta
    44941 aaaataaagc catccctaat aattgagata atgggatttt ttctaatgtg gatagatatg
    45001 aattattttt ctccttaagg atcatccgtt atttgggtcg ttttctgcta aaatctgcgc
    45061 cttgcgcttc tttagcgcac tgtctatggc taaccttttg aataaattca ttatgacgag
    45121 aatactcgct gcgataaccc ttttgctgag tatcgtattg accattttgg tcactatttt
    45181 ttgctccgtc ccgatcatca ttgccgggat tgtaaaacta ttgctacctg tgccagtcat
    45241 ttggcgaaag gtttcgcgtt tttgtgattt tatgatgtat tgctggtgtg aaggtctggc
    45301 ggtattactg catctgaacc cacacctgca atgggaagtt cacgggctgg aagggttaag
    45361 taagaagaac tggtatctgc ttatttgtaa ccaccgtagt tgggcagata ttgtcgtact
    45421 gtgcgtgctg tttcgtaagc acattccgat gaataaatat ttcctcaagc aacagctggc
    45481 ctgggtgcca tttcttggcc tggcgtgctg ggcgctggat atgccgttta tgaagcgtta
    45541 ttcccgcgct tatttattac gtcatccgga gcgacgtggt aaagatgttg aaaccactcg
    45601 gcgttcttgt gaaaagtttc gcctgcatcc caccaccatt gtcaatttcg ttgaaggctc
    45661 ccgtttcacg caagaaaaac atcagcaaac ccactccact tttcaaaact tgttgccacc
    45721 aaaggcggca ggcattgcga tggcgctaaa tgtactgggt aaacaattcg ataaactgct
    45781 taatgttacg ctgtgttatc cggacaataa ccgtcagccg ttcttcgata tgttaagcgg
    45841 taaactgacg cggattgtcg tacatgtgga tttacagcct attgccgatg agttacatgg
    45901 cgactacatc aatgacaaaa gctttaagcg ccattttcag caatggctga actcactgtg
    45961 gcaggaaaaa gaccggcttc tgacgtcgtt gatgtcatca cagcggcatg acaagtaaat
    46021 tttgctcgcc tctgaatgag cagaggcgag tgagtatttt aatgaacaaa acgtccggca
    46081 cgagacataa attcttcttc gctcatctcc tgtccgttaa aaacaacttt acccggtgta
    46141 taatgcagct gcagcgaggc ggtattgttg tcaatcgtga ttaactgaaa catctgacct
    46201 aatgtcatca tcccgctgat ttgtttatca gcctgctttt gcgctttttc cgcatccatt
    46261 ccttcagata aattaagctg ttttgcggtt tctgtgacga cattaagcgg taacttcaca
    46321 tcaaaattga gcgatttgat atctttgttt gtggatgatg aagatttggc tgggtcggca
    46381 atactgatat ccagattggc atttagttcg ccgagtgcgt tcttccatct taccggttgt
    46441 ttaatggtcg gctcactttt ttgtaataac ggtaaatact ctttgaacaa tgcagcattc
    46501 atttcttgca gagcaacttc atcgtttgct aactcagggt gtgcagcaag ctgcttctgc
    46561 atcgcaatgt tatactggat gataaattgg cgcactgctg agggatcaat agattcagca
    46621 atcaatgacc actcaccact accgagttgc tgattccctt ttgttaactt atcaagttcg
    46681 taggtcagat tgacattgat ataatctttg tctttaacgc gatccagcct ggtttttgca
    46741 acgaaagcat cgatttgggc aacgtcttcc ccctgattga taatattaac ctgattaaat
    46801 ttcgactcgc tttcaccaac cggaaatttc ttttcttcta atcgtgtcag agaatcaatg
    46861 gtaaaacttt ttgccgttac tttgttcatt ggtgataatt gcagaacaat atcttcgact
    46921 ttgccttcaa tatccaggtt agacaatgaa ctaccatcac cattaaaagt aaactggccc
    46981 tcaccgaaag aaaactttcc atactctgca gaggcaacat ctaatgtcgt agagaatgag
    47041 ccaccaaacg cgaatcgcaa tgtcgcttcc acgggcgatt tatttttcgc ggcgataaat
    47101 agcggttgtg tcagttcatt attcactaag ttcacttttg ccgctgccag tgctgggatg
    47161 acattaccat gcattaacat tgtgatgggc aacggaccgt gctcaacatc cacatcaaat
    47221 gcaactttct ggcctggctt gatattgaga tcgggtgcac cgttatcaaa tgaaatttgc
    47281 atttgaaaat gggaattaaa aaagcctttg tcaaagtttt tataacttaa cgtcatatcg
    47341 taggcatgtt cacctttctt tttagcatcg ttaaaatctt taataaattt ttcgacacca
    47401 ggctgaattt gcgtacctgt gtaccatgtg ccaccacccc agattacggc taacgcaaca
    47461 ataacacctg tagctgactt acgtatcata taaatattcc ctgataaaac agcaaaactt
    47521 tgaatatcca cttatgctga aataatgtcc agcggcagga tg
//
LOCUS       DAZFKF010000030        46353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFKF010000030 DAZFKF010000000
VERSION     DAZFKF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.030"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            480..605
                     /gene="timP"
                     /locus_tag="ACHKA1_002080"
     CDS             480..605
                     /gene="timP"
                     /locus_tag="ACHKA1_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO5679734.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNLSLSGNNLLVVNNLQNSR"
     gene            complement(637..1413)
                     /gene="sseB"
                     /locus_tag="ACHKA1_002081"
     CDS             complement(637..1413)
                     /gene="sseB"
                     /locus_tag="ACHKA1_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO5679735.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDVEEIIQAAGSVATDTLPADEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1556..2839)
                     /gene="pepB"
                     /locus_tag="ACHKA1_002082"
     CDS             complement(1556..2839)
                     /gene="pepB"
                     /locus_tag="ACHKA1_002082"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO5679736.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVFACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2898..3098)
                     /gene="iscX"
                     /locus_tag="ACHKA1_002083"
     CDS             complement(2898..3098)
                     /gene="iscX"
                     /locus_tag="ACHKA1_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO5679737.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3110..3445)
                     /gene="fdx"
                     /locus_tag="ACHKA1_002084"
     CDS             complement(3110..3445)
                     /gene="fdx"
                     /locus_tag="ACHKA1_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO5679738.1"
                     /translation="MPKIVILPHQELCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3447..5297)
                     /gene="hscA"
                     /locus_tag="ACHKA1_002085"
     CDS             complement(3447..5297)
                     /gene="hscA"
                     /locus_tag="ACHKA1_002085"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO5679739.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5314..5829)
                     /gene="hscB"
                     /locus_tag="ACHKA1_002086"
     CDS             complement(5314..5829)
                     /gene="hscB"
                     /locus_tag="ACHKA1_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO5679740.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5925..6248)
                     /gene="iscA"
                     /locus_tag="ACHKA1_002087"
     CDS             complement(5925..6248)
                     /gene="iscA"
                     /locus_tag="ACHKA1_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO5679741.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6265..6651)
                     /gene="iscU"
                     /locus_tag="ACHKA1_002088"
     CDS             complement(6265..6651)
                     /gene="iscU"
                     /locus_tag="ACHKA1_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO5679742.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6679..7893)
                     /gene="iscS"
                     /locus_tag="ACHKA1_002089"
     CDS             complement(6679..7893)
                     /gene="iscS"
                     /locus_tag="ACHKA1_002089"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO5679743.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8005..8493)
                     /gene="iscR"
                     /locus_tag="ACHKA1_002090"
     CDS             complement(8005..8493)
                     /gene="iscR"
                     /locus_tag="ACHKA1_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO5679744.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8764..9504)
                     /gene="trmJ"
                     /locus_tag="ACHKA1_002091"
     CDS             complement(8764..9504)
                     /gene="trmJ"
                     /locus_tag="ACHKA1_002091"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO5679745.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9623..10426
                     /gene="suhB"
                     /locus_tag="ACHKA1_002092"
     CDS             9623..10426
                     /gene="suhB"
                     /locus_tag="ACHKA1_002092"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO5679746.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            10571..11425
                     /locus_tag="ACHKA1_002093"
     CDS             10571..11425
                     /locus_tag="ACHKA1_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5679747.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKNGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            11616..12896
                     /gene="csiE"
                     /locus_tag="ACHKA1_002094"
     CDS             11616..12896
                     /gene="csiE"
                     /locus_tag="ACHKA1_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO5679748.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVCFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12888..14027)
                     /locus_tag="ACHKA1_002095"
     CDS             complement(12888..14027)
                     /locus_tag="ACHKA1_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO5679749.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14187..15077)
                     /gene="hcaR"
                     /locus_tag="ACHKA1_002096"
     CDS             complement(14187..15077)
                     /gene="hcaR"
                     /locus_tag="ACHKA1_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO5679750.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            15213..16574
                     /gene="hcaE"
                     /locus_tag="ACHKA1_002097"
     CDS             15213..16574
                     /gene="hcaE"
                     /locus_tag="ACHKA1_002097"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO5679751.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16571..17089
                     /gene="hcaF"
                     /locus_tag="ACHKA1_002098"
     CDS             16571..17089
                     /gene="hcaF"
                     /locus_tag="ACHKA1_002098"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO5679752.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17089..17409
                     /locus_tag="ACHKA1_002099"
     CDS             17089..17409
                     /locus_tag="ACHKA1_002099"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO5679753.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            17406..18218
                     /gene="hcaB"
                     /locus_tag="ACHKA1_002100"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             17406..18218
                     /gene="hcaB"
                     /locus_tag="ACHKA1_002100"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO5679754.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAAIGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTDPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18228..19430
                     /gene="hcaD"
                     /locus_tag="ACHKA1_002101"
     CDS             18228..19430
                     /gene="hcaD"
                     /locus_tag="ACHKA1_002101"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO5679755.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRGCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            19527..19949
                     /locus_tag="ACHKA1_002102"
     CDS             19527..19949
                     /locus_tag="ACHKA1_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO5679756.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(19997..20869)
                     /locus_tag="ACHKA1_002103"
     CDS             complement(19997..20869)
                     /locus_tag="ACHKA1_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO5679757.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNHFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(20881..21975)
                     /locus_tag="ACHKA1_002104"
     CDS             complement(20881..21975)
                     /locus_tag="ACHKA1_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO5679758.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMEHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(22008..23006)
                     /locus_tag="ACHKA1_002105"
     CDS             complement(22008..23006)
                     /locus_tag="ACHKA1_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5679759.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23030..24541)
                     /locus_tag="ACHKA1_002106"
     CDS             complement(23030..24541)
                     /locus_tag="ACHKA1_002106"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO5679760.1"
                     /translation="MFTATEAVPVAKMVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSGLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEIRALRHKP
                     KLDDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITHPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASRILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(24564..25547)
                     /locus_tag="ACHKA1_002107"
     CDS             complement(24564..25547)
                     /locus_tag="ACHKA1_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO5679761.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAAEKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQFVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEITQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(25644..28925)
                     /locus_tag="ACHKA1_002108"
     CDS             complement(25644..28925)
                     /locus_tag="ACHKA1_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO5679762.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDSLDYRCNLALAMLEYNR
                     ADFPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASKLL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICAKALGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            29043..30236
                     /locus_tag="ACHKA1_002109"
     CDS             29043..30236
                     /locus_tag="ACHKA1_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO5679763.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(30299..31552)
                     /gene="glyA"
                     /locus_tag="ACHKA1_002110"
     CDS             complement(30299..31552)
                     /gene="glyA"
                     /locus_tag="ACHKA1_002110"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO5679764.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            31880..33070
                     /gene="hmpA"
                     /locus_tag="ACHKA1_002111"
     CDS             31880..33070
                     /gene="hmpA"
                     /locus_tag="ACHKA1_002111"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO5679765.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33115..33453)
                     /gene="glnB"
                     /locus_tag="ACHKA1_002112"
     CDS             complement(33115..33453)
                     /gene="glnB"
                     /locus_tag="ACHKA1_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO5679766.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(33514..34848)
                     /gene="glrR"
                     /locus_tag="ACHKA1_002113"
     CDS             complement(33514..34848)
                     /gene="glrR"
                     /locus_tag="ACHKA1_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO5679767.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEMFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMVRGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(34838..35551)
                     /gene="qseG"
                     /locus_tag="ACHKA1_002114"
     CDS             complement(34838..35551)
                     /gene="qseG"
                     /locus_tag="ACHKA1_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO5679768.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDALRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(35716..37143)
                     /gene="qseE"
                     /locus_tag="ACHKA1_002115"
     CDS             complement(35716..37143)
                     /gene="qseE"
                     /locus_tag="ACHKA1_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO5679769.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(37719..41606)
                     /gene="purL"
                     /locus_tag="ACHKA1_002116"
                     /gene_synonym="purI"
     CDS             complement(37719..41606)
                     /gene="purL"
                     /locus_tag="ACHKA1_002116"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO5679770.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPVHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIYADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            41864..43420
                     /gene="mltF"
                     /locus_tag="ACHKA1_002117"
     CDS             41864..43420
                     /gene="mltF"
                     /locus_tag="ACHKA1_002117"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO5679771.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQHMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(43417..43920)
                     /gene="tadA"
                     /locus_tag="ACHKA1_002118"
     CDS             complement(43417..43920)
                     /gene="tadA"
                     /locus_tag="ACHKA1_002118"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO5679772.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(43978..44613)
                     /gene="yfhb"
                     /locus_tag="ACHKA1_002119"
     CDS             complement(43978..44613)
                     /gene="yfhb"
                     /locus_tag="ACHKA1_002119"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO5679773.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            44822..45670
                     /locus_tag="ACHKA1_002120"
     CDS             44822..45670
                     /locus_tag="ACHKA1_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO5679774.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
BASE COUNT        10990 a        12761 c        11680 g        10922 t
ORIGIN      
        1 cgacgttcat agaatggcgc aatatgttcg atattaaaat gacctggctt attagtccga
       61 attcagacaa atataaacaa atcccgctca atattaaaaa tactaaccgg taaaagatat
      121 tacttaaaca tgtaaattca ctttctttaa gcaacaaaaa accgccaaaa tcaggcggtt
      181 atttgttgcc ggtttggttc gcggcctctc cagcagattg acttgtgttg catgagcaac
      241 gcaggtgctt cacagcagag caatattcgc cagcaactct ctttttgtca agcaaaagag
      301 agtaattatt gtttatttag cgtattatcg acaccggccc tttccgccgt ctcgcaaacg
      361 ggcgctggct ttaggaaagg atgttccgtg gccgtaaatg caggtgtttc acagcgcttg
      421 ctatcgcggc aatatcgcca gtggtgctgt cgtgatgcgg tcttcgcatg gaccgcacaa
      481 tgaagatacg gtgcttttgt atcgtactta ttgtttctgg tgcgctgtta accgaggtaa
      541 ataataacct gagtctctcc ggcaacaatt tactggtggt taacaacctt cagaacagca
      601 ggtaagcccg aatgccgccc tttggggcgg catattttag attatccggt tctgtttaaa
      661 gtcacgcaaa aaaccacccc aacgacgttc atagaatggc gcaatatgtt cggtaataaa
      721 gtggctaatt cctttttccc cttttttcac ctgacaaata tcgattggtt catcggcagg
      781 taatgtatcg gtcgctacac ttcccgccgc ctgaataatt tcttcgacat caccatcggc
      841 ttcaatgcca ataagtaaat taggctgtgc ctcttcgttc tctttaattg aacatataaa
      901 agcacgcttc accggcttaa tagttttaaa taaggtggtg agtgaatcaa tcatttgtgc
      961 tggcggctct gcgacttccg ataatatcag cgattcaccg ccttccagga tttcctggct
     1021 gctcagcgga tttccctctt caccaatcaa caaactgatt tcacgcggca taaattcttt
     1081 accggtcggc agtttggcat taaggaagag cgtttcgcca agggtcatct caaacaatgt
     1141 gcgaacgggc attacgacaa atgcctgttc gtcttcaacc gcctgttgaa gtgcttctaa
     1201 cgaggtgaaa aaaggaataa cgctggtgcc gtcttctttt tcccagtgct gtaaatcaag
     1261 cgcgctatct tcaaccacag cctcgccctg cgccgccgta ccaggcaccc agacggtgga
     1321 ttccagtaga gtacggaaaa aggccgggcg gtgcgccggt tcggttgctg ctttttccag
     1381 caggtcttca agttcatttt ttgtttcgga cataagaacc acaattcatt caacgttcgg
     1441 ggcacaagtg cctgatgcgc catacttatc aggcctacaa gggagtcgca atgtattgaa
     1501 tttgcaatac tttgtaggcc ggataaggcg tttacgccgc atccggcata ttagtttacg
     1561 ccgttaacag attagcaatc gtgcgcacac caagtcccgt agcacccgca gaccactgtt
     1621 caaccggcgc tttacggtaa gtcgccgagc agtcgatatg cagccagcct tgctgatagt
     1681 tctcaacaaa gtgcgacagg aagcccgccg ccgtgctcgc gcctgccgga tacgccgcgc
     1741 ttccggtatt gttcagttcg gcaaagttag acggcagctg gctgcggtgg aactccgcca
     1801 gcggcagacg ccagaatggt tcgttctctt gtgaggcact cgccagcaaa cgaccggcca
     1861 gcgcatcgtc aaaactgaac agcgcatgat aatcattacc cagcgcggtt ttcgccgccc
     1921 cggtgagggt cgccgcatca atgatcagtt ccggtttctg cgcactggca tcaatcaggc
     1981 catcggccag caccagacgc ccttcggcat cagtgttcat cacttcaact tttttaccgt
     2041 tgcgataggt gatgatatcg cccagcttga acgcattgcc gctaatcagg ttatccgcac
     2101 agcagaggaa cagcttcacg cgcttgttca gtccgcgcgt aatggcaaat gccagcgccc
     2161 cggtaaccgt tgccgcgccg cccatgtccg acttcatcga gtccataaac gcagtctgtt
     2221 tgatgctgta gccgccggag tcaaaagtga tacctttacc taccaggcac gcgaacactg
     2281 gcgcttcttt atcgccagtt gggttgtaat ccagcgccag caataccgga gaacgttctg
     2341 aaccgcgtcc gacggtgtgc agccccatat aaccttgctc acgcagatct tcgcctttgg
     2401 tgatccgata agtcacacga tcgcccgcga cgttgctgat cagatcaaca gcacgctgtg
     2461 ccagttgcga tggtcccaat tcttctgccg gtgcgttgat ggtgtcacgc acccagtcga
     2521 tgatcatcag gcggttatcc agttcctggc gctgggcatc gtccagatcc ggccactcca
     2581 ctttacgcgt gcctttcggg gctttgtaac cttgccagaa tgcccagcag cgatccgcat
     2641 cccagccttc accgcttagc tgaacatgct tgatgcccag accgtcaatc ttgcgcgccg
     2701 cacgctggat cagccccaga tcgtctgccc cgttcaaatg cagagtaatg ccatcattat
     2761 taatgctgta agttgctttt tctccccagc gcgcgtcggc aggttgggta gagagggtaa
     2821 ttttcatcgc ttctgtcatt ttagttatcc ttattcgttt aacagcaaaa cgggctaccc
     2881 ccaggcagcc cgttcattca ctcggcttcg tccagccaga ctaacaaaat cgcttcgagg
     2941 attttctcgt tggatgcctg cgggtcgtcg tcgaaatctt ccagatcgca aatccactga
     3001 tgcatatcgg tgaatcgaac cgttttcgga tcaagatcgg gatacgcatc gtacagtgct
     3061 tcgccaattt cgcggctatc ggtccactta agtcccatac taacctctgt taatgctcac
     3121 gcgcatggtt gatagtgtaa cgcgggattt cgaccactaa atcttcgtcg gtaacgcgcg
     3181 cctggcagct taaacggctt tccggctcca gtccccaggc tttgtccagc atgtcgtctt
     3241 cctgctctga gctttccggc agcgagtcaa aaccttcgcg aacgatgcag tggcaggtgg
     3301 tgcaagcaca ggatttttca caggcgtgtt caatctcgat accgttacgc agagctgcgt
     3361 cgagaatggt ttcaccgcta ttagcttcca gaacagcgcc atcagggcag agctcctgat
     3421 gaggcaaaat aacaatcttt ggcatattaa acctcgtcca cggaatggcc tttcagcgca
     3481 cgacgaaccg actggtccat gcggcgagcg gcgaaatcct gggtttgttt gtctacgttt
     3541 ttaatcgctt gttcgatggc gtcaacatca tcgccctgcg ccacttcact caggtgagcg
     3601 gcagcatcgt caatgacctg acgttctgcg gcgcttaaca gcgcggcatc agcagccagc
     3661 gcgccgtgca gactttccag cacacgcgcc gcttctactt tttgttctgc cagcattcgg
     3721 gcttttacgt cctgctcggc atagctcatt gagtctttga tcatcgaagc gatttcgcta
     3781 tcggtcagac cgtaagacgg tttgacctga atagacgcct caacgccggt ggatttctcc
     3841 atcgccgtca cgctcaaaag accgtcggca tcgacctgga acgtcacgcg aatatgcgca
     3901 ccgccagccg gtagcgccgg aataccacgc agcgcaaaac gcgccagtga gcggcagtcc
     3961 tgcaccagtt cgcgctcacc ctgcattaca tggatagaca tcgccgtctg accatcttta
     4021 aaggtggtga aatcctgagc gcgggccacc ggaatagtgg tattacgcgg aatcactttc
     4081 tccaccaggc cgcccatcgt ttcgaggccc agcgacagtg ggatcacatc aagcaacagc
     4141 atttcgctgt ctggcttgtt acccaccaga atatccgcct gaatcgccgc gccaatagcg
     4201 acgactttat ccgggtcgat ggaagtcagc ggtggacgac cgaaaaattc gcctacccgt
     4261 tcacgcacca gcggcacgcg agtagaaccg cccaccatca ccacttccag cacttcatca
     4321 gcttctacac ccgcgtcttt cagcgcgcga cgacaagcca gtaaggttcg ttttaccagt
     4381 ggcgcgatca gttcattgaa ttgttcacgg ctgatttcgc cctgccagcc cgcaacgtta
     4441 acggtcacgg agtccgcatc gctcagcgcg attttggctg caatggcggc atccagcagt
     4501 tcacgctgaa cgcggttatc gctacgatca ggaatgcccg cctgctcgcg aatgtaatcc
     4561 gccagcagat ggtcgaaatc atcgccgccg agcgcggaat caccgccggt tgccagcact
     4621 tcaaacacgc cgcgacttaa gcgcagaatg gaaatatcaa acgtcccgcc accgaggtca
     4681 taaacggcga tcacgccttc ctgaccggaa tccagcccgt aggcgatagc cgcagcggtc
     4741 ggttcgttaa gtaagcgcag gacgtgaagg cccgccagac gcgccgcgtc tttggtgccc
     4801 tgacgctggg catcgtcaaa gtacgccgga acggtgataa cgacaccatc cagctcgcct
     4861 gccagggctt cagttgcccg cgccgccagt gctttgagga tgtccgcaga aacgcgcacc
     4921 gggttcagca gccccgccgc cgtttcaatc atcggcaggc cgttttcgct ggcctggaat
     4981 tgataaggca gatgcggata gcgttgctgg atatcagcca gcgagcgtcc catcaggcgt
     5041 ttaacagaac taattgtgtt ggcggtatcg agcgctgcat tagtacgcgc gtcataaccc
     5101 accgaatgcc cttgctgttg atagtgaaca acagatggca gcaggtgacg gccttcatgg
     5161 tcggctaacg tttcggcctg accgctgcgc actgtcgcca ccagcgagtt ggttgtgccc
     5221 aggtcaatac cggccgccag acgacgctga tgcggcgcag cactcaaacc aggttcacta
     5281 atttgtaata aggccatgtt tagcttccag aaattaaaaa tcgagcagtt tttcttcgag
     5341 ttgttcggca ctgcttcgca gtttatcgag aaaacgcagc ttacgcacgg tatccgccgc
     5401 cgcgtcccag gtctcgttgt ctaactgttc aaccatcaac tgatggcggg tatcaaacat
     5461 ctttttcaca cgtttgataa agctttccag ccgcgcttca tctttcgcct gttcgatctc
     5521 gtccagctct tcgcgcagct ccaactgttc catcaggaac gcggtgtcgc gcacagtatg
     5581 ctgctcgctg gcgagatcaa agccgtgcaa agaaagcaaa tattccgcgc gcattaacgg
     5641 atgacgcagc gtttgccagg cctggttaat ggttgcagat tgctgtacgg cggcgagttg
     5701 ttccgcctgg cttccgctgg cgaatttatc aggatgatac tgacgttgta gatcctgaaa
     5761 acgcaggctc agcgcctggg tatcgagttg ataacgggca ggcaagccaa agagggtgaa
     5821 gtaatccata acattctcag ggctgcgatc cgccgcgata acggcgaatc aggtaaaaca
     5881 aaccccacgc gcaggcgacc acggtggggt tatcggtatg cgcatcaaac gtggaagctt
     5941 tcgccgcaac cacactcatc tttaacgttc gggttggtga atttaaaccc ttcgttcagg
     6001 ccttctttta cgaagtccag ctgcgtaccg tccagaaatt gcaggctttt gccatcgacc
     6061 acgactttca cgcctttgtc ttcaaacacg atgtcttccg gcgtcggttc gtcaacaaat
     6121 tccagtacat aagccatacc tgaacatccg gaggttctca cgcccagacg caggccaaac
     6181 cctttgccgc ggttagccag aaaggtattt actcgcgctg ctgcactgtc gctcagtgta
     6241 atcgacataa ccaaacctca actcttattt tgcttcacgt ttgcttttat agtccgcaat
     6301 ggcggctttg atcgcgtctt ctgccagaat agaacagtga attttcaccg gcggcagttc
     6361 aagttcttca gcaatatcgg tgtttttgat cgcctgcgct tcgtcgagag acttcccttt
     6421 cacccattcg gtgaccaggg agctggaagc gatagcggaa ccgcagccgt aagttttaaa
     6481 acgcgcgtct tcaatgatac cttcatcgtt gactttaatc tgcaacttca tcacgtcgcc
     6541 acaggccggt gcacccacca tgccgctgcc gacgttctcg tcgttgttgt caaaggaacc
     6601 cacgttacgc ggattctcgt aatggtcgat aactttttcg ctgtaagcca ttataaattc
     6661 tcctgattcc gataccgatt aatgatgagc ccattcgatg ctgttcagat ccacgccctg
     6721 cttgtacatt tcccacagcg gagaaaggtc acgcagacga ccgatggatt tacgaactaa
     6781 ctcgatggtg tagtcgatct cttcttcagt agtaaaacga cctaaagaga aacggataga
     6841 gctatgtgcc agctcgtcgt tcagccccag cgcgcgcagc acgtaggacg gttcgaggct
     6901 tgctgacgta caggcggagc cggaagaaac cgcgaggtct ttcagcgcca taatcagcga
     6961 ctcaccttca acgtagttga agctgacgtt gagaatgttc ggcgcaccgt gttccaggtc
     7021 accgttcagg taaacttctt cgatatcttt gatgccgttc cacagacggt tacgcagtcc
     7081 gcgcagacgt tccatctcgg tcgccatctc ttcttttgcg atgcgatagg cttcgcccat
     7141 tccgacgatc tggtgaacag gcagagtgcc ggaacgcata ccgcgttcgt gaccgccgcc
     7201 gtgcatttgc gcttcgatgc gtacgcgcgg tttacgacgc acatacagcg caccaatacc
     7261 tttcgggcca tagattttgt gaccggagaa agacatcagg tcaactttca actggctcag
     7321 atcgataggc agtttgccca cgctctgggt tgcatcaacg tgatagataa tgccacgagc
     7381 acggcacatt tcgccgatag ccgcgatatc ctgcaccacg ccgatttcgt tattcacgtg
     7441 catgatagaa acgaggatgg tgtcgtcacg catcgccgct tcgagttctt tcaggtcgat
     7501 aataccgtta cgctgcggtg ccaggtaggt gacttcgaaa ccttcgcgct ccagctgacg
     7561 gcaggtatcc agtaccgctt tgtgttcggt tttgctggtg atgatgtgct tgcctttttt
     7621 ctgataaaag ttggctgcac ctttgatcgc caggttgtca gattcggttg caccagaggt
     7681 aaagacgatt tcacgcggat cagcgccgac cagatcggca atctgattac gggcgatatc
     7741 taccgcttct tcagcctgcc agccgaaacg gtgagaacgg gaggccgggt taccaaaggt
     7801 tccgtccatc gtcataaact gcatcatttt ctcggcaaca cgcgggtcca ccggcgtggt
     7861 tgcggagtag tcgagataaa tcggtaattt cattgctcta taaactccgt acatcactca
     7921 atgcaaggaa tcaggctacc ggctggatgt acgaccgtgt ttacggagta tttagcactc
     7981 cggcctgatt ctgaattctt tttattaagc gcgtaactta acgtcgatcg cgtcttgtgt
     8041 gcgggtgcgt ggcgcgtcgt gagtatgctg acgaccagac acatccagca cttcctggtt
     8101 attaaccagt tcgcctaaag taatgttgtt gagaaaaccg gtgagacggt cgctcaaatc
     8161 acgccacagc gcgtgggtca ggcatttatc cccgccctgg cagccgcctt taccctgaca
     8221 acgggtagca tctacagatt cgtcaacggc gctaatcact tcgccaacgg cgatgctgct
     8281 ggcatctttg cctaacagat aaccaccgcc tggtccacgt acgctggaaa ccagaccatt
     8341 tttacgcaga cgggaaaaca gttgttccag ataagaaagg gaaattccct gacgttcgga
     8401 aatatcagcc aacggtaccg ggcccgcttc agagttgagc gcaacgtcaa gcattgcggt
     8461 cacggcatag cgccctttag atgtcagtct catgtcttac ttcacctcaa actcgcccct
     8521 gcccggggtt ttttattgta aaagtggggg tattgcatag cagggtcaag tctgacattc
     8581 ccgagtaaat tggtcaacta tttacttgac tgatttagtc gggtatttaa ccttcagtgc
     8641 cattttttta tcgtggcgtt tgtacctgtt gtcggatgcg gtgtgaacac cttgtccgac
     8701 ctgggattcc gtgcacttac aggcctgata agacgcgtta agcatcgcat caggcaacca
     8761 ctgttattcg gccttattac ctttattctg ctgctctata gaagccagaa tcccgcgcag
     8821 gatattcaat tcctggcttt ccgggcgcgc acgggtaaac agacggcgca atttattcat
     8881 cacctgcccc ggatggtttt cacggataaa accggttgcc agcagcgttt gttccagatg
     8941 accataaaaa cgctccagat catcgaccag cggatacggc gtctcttcat gctcgacctg
     9001 ctcgccattt tcctgagttg ccagccaggc catacgcact tcataggcaa taacctgaac
     9061 cgccattgcc aggttcagcg aactgtattc cgggtttgcc gcaatcgcga catgataatg
     9121 gcatttctgc aactcttcat tggtcaagcc gacgcgctca cgaccaaaca ccagcgccac
     9181 cggggtattt gccgcttcag cgacactttt cagaccgcat tcgcgcgggt cgagcatcgg
     9241 ccacggcagc gtgcgggagc gtgcgctggt gcctaccacc aggctacaac cggctaacgc
     9301 ttcgtcgagc gtatcgacga tgtgagcatt accaatcaca tcgctggccc ccgccgccag
     9361 ggcaatagcc tgggagtcag gtttcaccag tggattaacc agccacagat tggttaatcc
     9421 cattgttttc atggcacggg caacagaacc catattgccg gtgtgtgacg tctccaccag
     9481 cacaattcga atattttgca gcattgtctt tcttcgtcta aagattattc gcgcatctta
     9541 tcataaaacg aagacagatg ccgatctcgc tgctatactc tgcgccgttt tcccgttctt
     9601 taacatccag tgagagagac cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg
     9661 caaggcgggt aatttaattg ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca
     9721 gaaaggcagt aacgatttcg tgaccaacgt agataaagct gccgaagcgg tgattatcga
     9781 cacgattcgt aaatcttacc cacagcacac catcatcacc gaagaaagcg gtgaacttga
     9841 aggtactgat caggatgttc aatgggttat cgatccactg gatggcacta ccaactttat
     9901 caaacgtctg ccgcacttcg cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt
     9961 tgctgtggta tacgatccta tgcgtaacga actgttcacc gccactcgcg gtcagggcgc
    10021 acagctgaac ggctaccgtc tgcgcggcag caccgctcgc gatctcgacg gtactattct
    10081 ggcgaccggc ttcccgttca aagcaaaaca gtacgccact acctacatca acatcgtcgg
    10141 caaactgttc aacgaatgtg cagacttccg tcgtaccggt tctgcggcgc tggatctggc
    10201 ttacgtcgct gcgggtcgtg ttgacggttt ctttgaaatc ggtctgcgcc cgtgggactt
    10261 cgctgcaggc gagctgctgg ttcgtgaagc gggcggcatc gtcagcgact tcaccggtgg
    10321 tcataactat atgctgaccg gtaacatcgt tgctggtaac ccgcgtgttg ttaaagccat
    10381 gctggcgaac atgcgtgacg agttaagcga cgctctgaag cgttaatgac tcaggcgggt
    10441 gatatcactc acccgccctc gcctttcagg cgctattccg aaatacttcc tcaccgcttt
    10501 actttctttc gtcactctcc caccatcttt ccccgattaa tggatgataa agaagtcgca
    10561 aggaaaggtt atggcactgc cagtgaacaa acgcgttctc aaaattctgt ttattctctt
    10621 tgttgttgcc ttctgcgttt atttagtgcc gcgcgttgcc atcaacttct tctattatcc
    10681 tgacgataaa atttacggtc ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa
    10741 gaacggtact cgtctgcaag gctggtttat cccttcttcg acgggccctg ctgacaacgc
    10801 catcgcaacc atcattcatg ctcacggcaa tgccggaaat atgtccgccc actggccgct
    10861 ggtcagttgg ttacccgagc gtaatttcaa cgtttttatg tttgattatc gcgggtttgg
    10921 taaatcaaaa ggcacgccgt cccaggccgg attgctggac gatacgcaaa gcgccatcaa
    10981 tgtggtgcgc catcgcagtg atgtaaaccc acaacggctg gtgctgttcg ggcagagtat
    11041 tggcggggcg aatattcttg ctgttattgg tcagggtgat cgtgaaggca tacgtgcggt
    11101 gatcctcgac tccacatttg cctcttatgc aaccatcgcc aaccagatga tccccggcag
    11161 tggctactta cttgatgaga gttacagcgg cgaaaattac atcgccagcg tcagcccgat
    11221 cccgctttta ctcattcacg gtaaagctga tcacgttatc ccatggcagc acagcgaaaa
    11281 gttgtatagc ctggcaaaag agccaaaacg gttgatccta atcccggatg gcgaacacat
    11341 tgatgctttt tccgatcgtc acggcgatgt ttatcgcgaa cagatggtgg actttatcct
    11401 tagcgcgttg aatccgcaga actaacccat gatcgctagc acgataatca ttcacaaaac
    11461 caccttaaga catgctaatc cactggtcag aacagtttaa gatgagaaaa attctgtgac
    11521 gcttgccaac atttctgatg attagcattc ccttcgccat ttccctgagc aaactttagc
    11581 tattcttatc aattatgctt atgggagatc tacagatgat gcctacgctt gctccaccat
    11641 ctgtcctttc ggctccccag cgccgctgcc agatcttgct gacgctcttt cagccggggt
    11701 tgaccgccac catggcaacc ttcagcgagc ttaatggtgt ggatgatgat attgccagtc
    11761 ttgatatcag cgaaacagga cgggagatcc tgcgctatca tcaactcaca ctgacgactg
    11821 gttatgacgg tagctaccgg gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc
    11881 actggctacg acgtggtttc cgtctgtgtc cgtcatttat taccagccaa ttcacccccg
    11941 ccctgaagag tgaactgaag cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc
    12001 tacaagcgtt agtgaatctc tgctcccgac ggctgcaaaa acgctttgaa tcgcgcgata
    12061 ttcatttcct gtgtctgtat ctgcaatatt gtttgctgca gcaccacgct ggaattacgc
    12121 ctcagtttaa tccgctccaa cgtcgctggg cggaatcctg ccttgaattt caggtagcgc
    12181 aggaaattgg acgccactgg cagcgtcggg cgctccagcc tgtaccacct gatgagccac
    12241 tgtttatggc actacttttt tccatgttgc gggttcccga tccattgcgg gatgcgcatc
    12301 agcgggacag acaattgcgt cagtctatca aacgtctggt aaaccatttt cgtgagctgg
    12361 gaaatgtttg tttttatgat gaacaggggt tatgtgatca gctttatacc cacctcgccc
    12421 aggcgttaaa tcgcagtttg tttgccatcg gtattgataa taccctgccg gaagagttcg
    12481 ccagactgta tccacgcctg gtacgcacca cccgcgcggc gctggccgga tttgaaagtg
    12541 aatacggcgt ccatctttct gatgaggaaa gtggtctggt cgcggtgatt ttcggtgcct
    12601 ggctaatgca ggaaaacgat ctgcatgaaa aacagattat tctactgacc gggaatgata
    12661 gcgagcgaga agcgcagatt gagcagcagt tacgcgaact aacgttactg ccgctcaaca
    12721 ttaaacatat gtcggtaaag gcatttttgc agacaggcgc tccgcgtggc gcggcactga
    12781 ttattgctcc ttataccatg ccgttaccgc tcttttcacc accgctgatc tatacggacc
    12841 tgacactgac aacacatcaa caggagcaga tccgcaaaat gctcgaatca gcatgaggga
    12901 acaactttcg ggcgcaaaaa cattgccgga agcgccacca gcgccatcac ccagaacacg
    12961 ccgtggccca gatattgata caggaaaccg gcgaaaacgg tcatgatagc gatactgccg
    13021 cccatcgcga cggcagagta aaccgcctgt aaacggatga cttcgctacc ctggcgagcg
    13081 gcaatatagc gcatggcggc caggtggcag accgtgaagg tgccgcaatg cagaatttgc
    13141 accactatca accacggcaa cgccgtagtt gctcccataa tgccccagcg cactacgccg
    13201 caaatcgccg agatcaacag catatcgcgt gcactacaac ggcggaaaag tttattactc
    13261 agtgcaaaga taatgacttc cgccaccacg cccagcgacc acaaataccc caccgccgag
    13321 gccgagtagc cagctgcctg ccagtaaatg gcgctaaaac cgtaataggc cgcatgcgcc
    13381 ccctgcaata aacaaacgca ggccagaaag cgccagttct ggcgaaccag cgccaaccac
    13441 gctgaccaac cggtgctctc ctgttggcgg cttgcccctt gtggctgaat cgtcggacgg
    13501 atgagaaagc cgagcagcat ggatgccacg cccaacgtca acagcacgag gatcacccga
    13561 taatcaaaca tagtgaccag tttgcccgtc agcgccgagc caatgacaaa cgccaccgag
    13621 ccccacagtc gcactttgcc gtaatcaagc gggaactgct tttgccacgt attcgccagt
    13681 gcatcggtca acggtaccag cggtgagaaa aagaggttaa agccaatcat caccagcatc
    13741 agccacgcta cgtgcgcccc cgcccagaag gcgacagcaa agagaagtgt cagcagtgcc
    13801 agcacgcgca aggcggaaat caggcgagaa ggatcgctga cgcggggcgc gatgagcaaa
    13861 ctcccgagaa aacgggcaac cagacctgcc cccaataaca ggccgatggt ttctggcgtt
    13921 aaaccaatcc ctttaagcca gacgctccag aaaggtagaa aaatgccgta actaaaaaag
    13981 tatgtgaaat agccgagcgc caaccagcgc gtggattgca aaaccatgag tccctcccgt
    14041 caggaggcgt tagtctggag ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc
    14101 gagcctgctt cccgatgtct gtaacgccag ttcacaagca ctcaaaaact gcgatccgtg
    14161 acgcatgttg caaagataat gtgtaattat gccgttacgc ttgccaaacg ttcctgcaca
    14221 atggcgatga aatcgcgtaa tgccggcttc atctcccctt tcttccacgc catcagtaaa
    14281 gcaatggaag gcacatttcc ggcaatggga cgaaaaacaa cctgcccggt attaaaatta
    14341 ttcatataac cgggtatcaa agtgacgccc agccccatgc ccaccagatt catggtcacc
    14401 agaatattcg ttgccacctg gacgatattt ggctggctgt tttcttgtgc aaaccacgct
    14461 ttaacgatcg gcgcaagcga accggaatac gccggatcgg tactgacgaa attcacgcca
    14521 tccagttgcg cggcggtgat ctctttttca tgtgctaaag ggtgatcaac cggtaacacc
    14581 accactaatg gttcgtcaaa aagctccaga taatcaatct ccgggctata aacaggatgg
    14641 cgcattaagc cgacgtcgag ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt
    14701 aaactcacca gctcaatcaa ggtgtctggc tgtctgagac gaaacatcgg taatactttt
    14761 ggcagtaaat tcacttccgc cgatggcaca aagccaatgg ttaattgtct gtcttcctga
    14821 acaattttcc gcgcccgtaa tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc
    14881 tggagaaaac attctcccgc cgccgtcaac gcgactttgc gcttatccct caccagtaac
    14941 ggaacaccga cacagttttc aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta
    15001 tgcagttttt ccgccgcacg ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt
    15061 aaatgccgta gttccatcac cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa
    15121 ttaactattt cacatattag caaccaacca gcaacatcct tatggcacaa aaatagaagg
    15181 tcaatacatc ttatctttca ggattaaaaa atatgaccac accctcagat ttgaacattt
    15241 accaactgat tgacacccaa aacggtcggg tcactccgcg tatttatacc gacccggaca
    15301 tttaccaact ggagcttgag cgtattttcg gtcgttgctg gctatttctc gcccacgaaa
    15361 gccagatccc aaaacccggt gatttcttta acacctacat gggagaagat gcagttgtcg
    15421 tggtgcgcca gaaagacggc agcatcaagg cgtttctcaa ccaatgccgc caccgggcca
    15481 tgcgtgtgag ttatgcagat tgcggcaaca ctcgcgcctt tacctgcccg tatcacggct
    15541 ggtcttatgg cattaacggc gagttgatcg atgtaccgct ggaacctcgc gcctacccac
    15601 aagggttgtg taaatcccac tgggggctaa acgaagttcc ttgtgtggag agttataaag
    15661 ggctgatttt tggcaactgg gataccagcg caccgggcct gcgtgattac ctgggtgaca
    15721 ttgcctggta tctggatggc atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg
    15781 gcgtacaaaa gtgggtgatc aactgcaact ggaaattccc ggcagagcag ttcgccagtg
    15841 accagtatca tgctctgttc agccatgctt ctgccgttca ggtattaggg gcgaaagatg
    15901 atggcagcga taagcgcctc ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca
    15961 aagatgcgct gcaatttggt caggacggtc acggtagcgg tttcttcttt actgaaaaac
    16021 cggatgctaa tgtctgggtc gatggcgcag tttcaagcta ttaccgcgaa acctatgccg
    16081 aagcagaaca acgtttaggt gaagttcgcg ccctgcgcct ggcgggtcat aacaatattt
    16141 tccccacgct ttcatggctc aacggcactg ccacgctccg cgtctggcat ccgcgcggcc
    16201 ctgatcaagt cgaagtgtgg gcgttctgta ttactgacaa agccgcctcc gatgaagtta
    16261 aagccgcttt tgaaaacagc gccactcgtg cttttggtcc tgctggtttt ctcgagcagg
    16321 atgactcgga gaactggtgt gaaatccaga aattgcttaa aggccaccgc gcccgcaaca
    16381 gcaaactgtg tctggaaatg gggcttggtc aggaaaagcg ccgcgacgac ggcattcctg
    16441 gcattactaa ctatatcttt tcagaaactg ccgctcgtgg aatgtaccaa cgctgggccg
    16501 atcttctgag tagcgaaagc tggcaggaag tgctcgataa aaccgccgct taccagcagg
    16561 aggtgatgaa atgagtgcgc aagtttcact agagttacat caccgcatta gccagtttct
    16621 ctttcacgaa gccagcttac tggacgactg gaaatttcgt gactggctgg cgcagctcga
    16681 cgaagagatt cgttacacca tgcgcaccac ggttaacgcg caaacgcgcg accgccgcaa
    16741 aggcgtccag ccgccgacga cctggatttt taatgacact aaagaccagc tggagcggcg
    16801 aatcgcccgt ctggaaacgg gcatggcctg ggcagaagag ccaccgtcac gcacccgtca
    16861 cttaatcagc aactgccaga taagcgaaac cgacatccca aacgtatttg ctgttcgggt
    16921 gaattatctg ctttatcggg cacaaaaaga gcgcgatgaa acgttctatg tcgggactcg
    16981 ttttgacaaa gttcgccgtc tggaagatga caactggcgc ttgctggaac gggatatcgt
    17041 cctggatcaa gcggtaatca cttcccataa cctgagtgta ctgttctgat gaatcgaatt
    17101 tatgcgtgtc ccgttgcgga tgtgccggag ggtgaggctc tccggatcga tacctcgccc
    17161 gtcatcgccc tgttcaacgt tggcggcgag ttttatgcca ttaacgatcg ttgcagccat
    17221 ggtaatgcgt cgatgtcaga agggtatctg gaagatgacg ccacggtgga gtgcccgcta
    17281 cacgccgcca gtttttgcct gaaaacgggg aaagcgttat gcctgcccgc caccgatccg
    17341 ctcaccactt atccagtaca cgttgaaggt ggtgacattt tcatcgactt accggaggcg
    17401 cagccatgag cgatctgcat aacgagtcca tttttattac cggcggcgga tcgggattag
    17461 ggctggcgct ggtcgagcga tttatcgaag aaggcgcgca ggttgccacg ctggaactgt
    17521 cggcggcgaa agtcgccagt ctgcgtcagc gatttggtga acatattctg gcggtggaag
    17581 gcaacgtgac ctgttatgcc gattatcaac gcgcggtcga tcagatcctg actcgttccg
    17641 gcaagctgga ttgttttatc ggcaatgcgg gcatctggga tcacaatgcc tcactggtta
    17701 atactcccgc agagacgctc gaaaccggct tccacgagct gtttaacgtc aacgtactcg
    17761 gttacctgct gggtgcaaaa gcctgcgctc cggcgttaat cgccggtgaa ggcagcatga
    17821 ttttcacact atcaaatgcc gcctggtatc ccggcggcgg tggcccgctg tacaccgcca
    17881 gtaaacatgc cgcaatcggg cttattcgcc aactggctta tgaactggct ccgaaagtac
    17941 gggtgaatgg cgtcggcccg tgtggtatgg ccagcgacct gcgcggccca caggcgctcg
    18001 ggcaaagcga aacctcgata atgcagtctc tgacgccgga gaaaattgcc gccattttac
    18061 cgctgcaatt tttcccgcaa ccggcggatt ttacggatcc gtatgtgatg ttggcatcgc
    18121 ggcgcaataa tcgcgcatta agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc
    18181 gcggcattcg ccacgtagct gctgggctgg atctttaagg aagtacgatg aaagaaaaaa
    18241 cgatcattat tgtcggtggt gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc
    18301 aagggttcac cggtgagctg catctgtttt ccgatgagca acatcttcct tatgaacgcc
    18361 ctccgctctc gaaatccatg ttgctggaag attccccaca gttgcagtct gtgttacccg
    18421 ctcactggtg gcaggaaaac aatgttcatc tgcattccgg tgtaaccatc aaaacattgg
    18481 gccgcgacac acgagagtta gtgttagcca acggcgaaag ctggcactgg gatcagcttt
    18541 ttatagcaac cggcgcggca gctcgaccgc tgccgttgct tgatgcactg ggagaacgct
    18601 gctttacctt gcgccatgcc ggtgatgccg ccagactgcg agaagttctg cagccagaac
    18661 ggtcagtcgt aattgtcggt gccggaacta ttggtctgga actggctgcc agcgccacgc
    18721 agcgcggatg taaggtgaca gtgattgaac tggcggcaac cgtcatgggc cgtaatgcac
    18781 caccgcccgt gcaacgctat cttttacagc gccatcaaca ggctggtgtg cgcattctgc
    18841 tcaataatgc cattgaacat gtggtcgatg gtgaaaaagt agaactgacg ctgcaaagtg
    18901 gcgagacgct tcaggccgat gtggtgattt acggtattgg tatcagcgcc aacgagcaac
    18961 tggctcgcga ggccaacctt gatactacca atggcattgt cattgatgag gcttgccgca
    19021 cctgcgatcc cgcgatcttt gccggtggcg atgtggcaat cactcgtctt gataatggtg
    19081 cactacaccg ctgcgaaagc tgggaaaacg ccaataacca ggcgcaaatt gccgctgccg
    19141 caatgttagg gctaccgctt ccgctactgc cgccgccgtg gttctggagt gatcagtaca
    19201 gtgataactt acagtttatt ggcgatatgc gtggcgatga ctggctttgt cgtggcaacc
    19261 cggaaaccca gaaagcgatt tggtttaatc tgcaaaacgg cgtgcttatc ggtgcggtaa
    19321 cgctgaatca gggacgtgag attcgctcaa tccgcaaatg gatccagagc ggcaaaacgt
    19381 ttgatgcgaa acagctgaca gatgagaaca tcgcgcttaa atcactgtaa ccaggataat
    19441 tagcgaatat ctcaacgcct ggggcgtggc gaggtaccag agtgtgtatt acgtttaaat
    19501 cacattatct tgcaaaggga ttggttatga actcactacg ttattttgat tttggtgctg
    19561 cccgccccgt tttgttatta attgcccgta tcgccgtggt cctaattttc attatttttg
    19621 gttttcccaa aatgatgggc tttgacggta cggtccaata tatggcctcg ctgggcgcac
    19681 cgatgccgat gctggcagcg attattgcgg tagttatgga agtgcccgcc gcgatattaa
    19741 tcgtgcttgg ctttttcacc cgtccgctgg cggtactgtt tattttctac acgctgggta
    19801 cggcggtgat tggtcaccat tactgggata tgaccggcga tgccgtcggg ccaaatatga
    19861 ttaatttctg gaagaatgtc agtatcgctg gcgcgttctt gctattggca attaccgggc
    19921 cgggggcaat ttctctcgat cgccgttagg aaagatgccg gatgcggcgt gaacgcctta
    19981 tccggcattt tataaattac agccactcta cccgcaacga catctctgag gttgttgatt
    20041 cccccggcgc aagcgcaatg aggtcacccc cttccgggcg atgatggtca tccggcgcat
    20101 ggctcatcgg ctccagacag aagaaatcaa acgcatatcc tttatcaaac gccgggtcgg
    20161 aaacaaagat gaaataacac ggtgcaggtg gcgtcgtttc tatgatgatg gcataaccct
    20221 cctgcggctg ctcgatgcgg gcctgaccat tccatccggc aaaaccattg ttcacccact
    20281 ggcgcggcaa cggcgctagc tggttaaaat ccagttcttg cggtagctgc tcgcaaaact
    20341 cacccgccag ccactgctcc cgctccagcc agtaaccgct cgcctgtgcc tgaatccgcg
    20401 tttgcggcga caacggaaaa taaggatgcc agccggtacc aaatggcagc gtctctgccc
    20461 cttgattggt gacagagagc gtcaccgtca gcgtatccgc cgttaaatga aacgcctgac
    20521 ttacccgata gtgatagaca ccgctgcgat gttcatacac cagacacaaa ctatcatcgc
    20581 tgtgcgagac acattgccac tcgcccagcc agccatcgcc gtgtagatag tgcgcatccc
    20641 actcaacatt cggttgcagt tgatactcac gcccctgcca gacaaaatgg ttgccgctca
    20701 cccggtttgc aaatggcact agcggaaaac aagaggcatc ggttgccaca ccgctttttt
    20761 taccaggacg taagagcggc gtcgtatcac gccagaaacc ttcgatgaca ccgccctggt
    20821 cagagacatc cagctttagc ggtccgtgag ataaggtata gatggtcatc cgctaactcc
    20881 ttaatccggg aagttaatca caactttccc gcatttgccg ctcgccatca gcgcataggc
    20941 atcacctgcc tgttccagcg agaagcgatg ggtaatggcg ttacgcggcc acagcttcca
    21001 gtcagtcaaa tcgtgggcgc atttttccat atggaacaga ctggtcaccc aggagccaat
    21061 aatccgccgt tgatggtgca tcagatcggc gctgacctcg aattccactt ttccggtttc
    21121 accaatgtaa accacccgcc cccagtcagc agtggattgc agtgccagca agcgacctgc
    21181 ggcattaccg gaacaatcga gcgcaacatc cgcgccaccg tgggtaagtt cagcaattat
    21241 ctgcggcaga ccttcggtgg tcgctaaata accgtgctcc atcaccccta actgttttgc
    21301 catcgccaga cgttccggca gcatatcaac gccgatgatc cgttttgcac cgcgaccttt
    21361 cgccagcatc atcgccatca tgccgaccgg ccccagaccg accaccagca cgttatcgct
    21421 gccggaaact tcgccgcgca aaatcccttc ataggccgtg ccaacgccgc aactgataaa
    21481 cgcaccatct tcgtagctca gcgcatccgg caggaggatc agatcttttt cttccgccag
    21541 cagatattcg gcatgaccgc cgtcacgctg ccagccgtaa gccgcttttc ctttgccagt
    21601 acaagagata ggaaagccgc ggcggcagtt cgggcaaaaa ccacagccag aaatgtgata
    21661 caccagcacg cgatcaccct ctttaaaatg gcgacagcct tgccccatcg ccacaatttg
    21721 cccgcacggc tcatgaccgt tgataaagcc ctggtataac ggtttatcgg gcgccgccgc
    21781 cgtggcgcgg tgttgatggt agatatagtg gacatcgctt ccgcaaatcc cggaggattt
    21841 cattttgatc agtacctggt taatccccgg cgtcggcacc gcaacttccc gcagatcgac
    21901 ggtcgaattt cctggtaaat aagctgccag catcgttttc ataaatccct cattaacaat
    21961 acgattaatt ttcatccctg ccgcacccgc gccaggggcg ttaaggttta gcgtttcgct
    22021 ttactgctac gctgggtcag caagatattc gccagcaccg ccaccacgat gatgacgccg
    22081 cgcaccacct gctggaaaaa ggagttaatc ccgagcagca ccagaccgtt accgattagc
    22141 gtaatcacca gtacgccaag cagcgtaccg aacaacgagc cgcgaccacc ggaaagcgcc
    22201 gtaccgccga ccacgaccgc ggcgatgacg tcaaactcca gaccgtttgc ggcacctgcg
    22261 ttaccggaac cgaggcgcgc cgccaacaaa atgccggtca ccgccgctaa taatcccgaa
    22321 agggtaaaga taagaatgcg tacccgacga acgttaatac cgcacaactg cgccgccgtg
    22381 gcattaccgc caacggcaaa aaccgagcgc ccgaaggcgg tttttcggct aatgaacaca
    22441 aacagcgcaa acagcaccat catgatcagc gcggataccg gcacaccgag aaattgtccg
    22501 cccagccagt ccagcacctc gttttcgtca atcggcactg gcagggcgtt ggtcataaac
    22561 aagcccattc cgcgcagggc gctccacagc cccagcgtgg ccacgaaact tggcacgtta
    22621 aacacgccac gcagcacccc ggcgagcgtt cccatcagcg cgcctaacag caacaccagc
    22681 agacaagcaa tcgccaacgg aacttcaaat tgcagcaaaa atgccaggca caccgagaca
    22741 aaagccacca tcggcccaac gctgacatca atttcaccgg agataataat cagcgtcatc
    22801 gcccaggcgg caatcccaat ggtggcggca tcgcgcagca cgttcatctg gttattcaat
    22861 gagataaagc caggcgcgtt cagggagaag accagataaa gaatggcaat caccaccagc
    22921 aaaccgatct cattaatatg gcgactgaca aattgtttga gcgagacgct cttgccctgc
    22981 ggcaatggta atgacgaagc agacatctta ggttcctcgg taaaattcat cagtgcacag
    23041 acagaatggc ggacatcagc tcatccacat tgaccggagc gtgaaactcc tgcgagaacg
    23101 taccgtgctg taacaacaga atgcggtcgc acaccagcgg taactcctcc acttcactgg
    23161 agataaacac cacgcttttt ccttcggcag ccagctcacg gacaatacgg taaatctgct
    23221 gtttggcttc gatatcgacg ccgcgcgtcg gctcgtcgag caacaaaatc cggctggcgg
    23281 catagaccca acgaccgatc accacttttt gctgattgcc gccagaaagc gtaccgatgg
    23341 gcgtttcact actggcggcc ttgaccgtca tccgctgcat cacctcttcg gttaggcggc
    23401 ggatggtgga ccattgcagc acaccgttgg cgctgatttt ttgccgattg gtcagcactg
    23461 tattttcgtc aacgcccaac cagggaatga tccccgcttc tttgcggttt tctggcgtat
    23521 agccaatgcc gcgtttcagc atgtcgccgt aatcggggtg cgtgattttc tcgccgttga
    23581 taacaatttc gccctgttca tactcctcca gcccaacaat cgctttcagc aattcactgc
    23641 gtcctgcccc cagcaggcca gcaatgccga gcacttcgcc acgacgtagc gtaaagctga
    23701 tgtcgtccag cttgggctta tggcgtaacg cacggatttc cagcacggcc tgatccacaa
    23761 tttcctgagg ggcaaccggc gcaatatcaa cgtgatcgcg cccgagcatc agcgacacaa
    23821 tatgatgcgt ggaggtgttt tcgagcatca catcgcccgc cacctgacca tcgcgcatga
    23881 cggtggcaca ggaggcaatg cggcgaattt cttccatccg gtggctgaca taaatcaccg
    23941 ccacgcccag tgccgacatc tttttcacgg cgcttatcac cagttcaact tccgcactgg
    24001 caagcgaact ggtaggttca tcaagaatga ccacgcgcgg ttcgcctttc atcacccgcg
    24061 caatttccac cagctgcttt tgcgccgggc ttagccctga aacaagttgt tcagggctaa
    24121 cgtcaacgcc cagcgcctgt aagcaacgtt gggcatcctg cgccatttgc aggtaatcaa
    24181 tcatgccgtt gcggcggggc cactgaccga ggcagaggtt ttccgccact gtcagccctt
    24241 ccaccaaact taactcctga taaaccgcgc gtacccccag ttcagcggca cggcgagtca
    24301 acgtagcttc gtcgccttcc agtcgcgtct caccaatcca gatatcaccg ctatccggac
    24361 gttcgctacc ggtaagcatt cgaatgaggg tcgatttgcc cgcgccgttt ttgcctaaca
    24421 gcgcacgaac ctcgccttta ttgagcgtga agttaacgtt atccaacgca acgacgccgg
    24481 gataacgctt atttcctgcc accatttttg ctaccgggac tgcctctgtt gccgtgaaca
    24541 tagggacctc tgcgaatcag cgattagggc agaccatcaa cgtgcgttgc cagccactgt
    24601 ttaccgtctt ccgttttggt atagagatcg attggcacct gaatcacttt ttcaccgtcg
    24661 gcttgtttat tgataacctt caatgtttgc gcgaaaacag cattgcccat tttcttaccg
    24721 gaaatatcca ctaccgcttt cagcacctga ttgttttcca gctcctgagt aatttcggtt
    24781 gtcatatccg aaccgaaaac agcaattttt ccggcctgat tttgattacg taccgctttt
    24841 accgcgccga gtgtcgcacc gcccgattcc cccataatgg cgttgagatc cggcgtggag
    24901 ataatcagtt tttcaccaac ggaaatcgct ttatctaaaa cagtcccttc ctgattagcg
    24961 acaaattgcg cgccgggaac gcgggctttt aatacttctt caaacccttt acgtcgttgc
    25021 acacaaactt caaaggcttc gcaattgatg acggcaattt tcggctggtc aattttgttg
    25081 gcaataaaat aatcggcggc agcgttaccc agttttttac caaattccag cgggtcaccc
    25141 accagatatg ccgagacata tttatcgacc cccttttgat taatacaggt gttgtagcaa
    25201 atcaccggaa tacccgcttc actggcgcga cgaacggtac ggctactgcc attttcagac
    25261 actgccgata aaataatggc atcgacatta cgcgccacga gggtatcaac aaaggtactt
    25321 tctttcgaaa tatcaccctg ggcgttagtt tcaattaact gtacctgtac tgaagaatct
    25381 tttgccgcat cctgaacgcc ctgacgcact ccagcgtaat atccctgagt atcaaggtat
    25441 attgcgccaa tggtcatttc tttttccgca gccctggcaa ataaagcgct acctaatagc
    25501 gtagccatta ataatagatt acgggttgtt ctcatttttt taggcataga gcctcctgta
    25561 gggtttttat taacaacggc ttattctaat tattttgtga tgagcggcag cgcgtgccgc
    25621 tcaaatatta cagttaatag ggtttagtga acgtagttaa aaataaaagg catcacggtg
    25681 gtgaataatg ccgctttcgg ccaggccgga ttaatcgccg tcaattcagc gcgagcagac
    25741 tcatattgcg caacctcccc gagtcccgcg gacgccagca tcgccaccat caggcatttt
    25801 tctttatgct gctgttgtaa atcgccatac agcgacaaca gatcaggttg tgaaacagca
    25861 aagaaatccg cctcaatact ggttttcgcc atctcttgcg cccactgttt catttcacta
    25921 aacagttgct gtgcggtgtg ttgttcgccc agtaatcgca gcgccattcc ttgccagaag
    25981 aggtaatcaa ccggctgatc gttgtaatag ctgtggatgt taatggtgcg atcgccggtc
    26041 gccgccagac gtaaacagcg cgtcgcttca gtttcatcac ccagggcttt ggcacatatc
    26101 gcctgccaga accagatgtc gttgtcagtt tgccccggta atcggccttc gcttaaattc
    26161 tccggatagt gcagcgcggc atgaagcagt ttgctggcct gctgcggttg tctggcatca
    26221 aggtgttgcc aggcgcgtaa caactggttg aggataaact gactggtgac tttcccttct
    26281 ccgccttccc acgggtggaa tttgcgcgtg gcgagaatgt ccgccgcttt gtctgcctgc
    26341 cccgtgagat gccacaaatt gagcagttct gcggtcatgt cgtcgcgttt cagcgcaatt
    26401 tccagattat tttccagacg cgccagtcgt ttctccggtg tggctccact taacttatca
    26461 agcaaatccc gttcgaaaag cagacgtgca tcctgcggcg caagctgata agcattatca
    26521 agataacgcg cggccagctc ataatcgtgt tgcttattcc acgcatggat cgctaacccg
    26581 cgccagccgt cggcaaactc cggcgacatc tctacgcaac gttgccataa ggcaatggct
    26641 ttgttgtagc tacgtttgtt gtagtagaag caggccagta aatggcgagc aaaccagcac
    26701 tcttcaatac tctccagcgc cgccacttct tccagcgtat tcgggaaacg gacaaactgc
    26761 gggaagacat caatggcttt tgcgaccagt tcgccacgtt cggctttcgg cagcaagctg
    26821 gcttgcaggt aaagcggcag cgtgcgctgg cagtccagtg cgttcagcat ctctgccgcc
    26881 agggtgggca ttccccagtt aatcagttgc ccggcggtca tcagggcgtt aacgtcgcgt
    26941 ccctgacaca gaccgcgcca ctgcgcgaga gctgattcgc tacgaccatc gaaccagttc
    27001 agccaccaca gagtggcgtt cagcggataa tcgcgcagca gtttctcgcg ctgcaaacgc
    27061 gcgttgtcct gacgaccact taacaccagc agcagattat gcaggcaaag cacttcctga
    27121 ttggttgggc aggcgcgaag actttgttgg caaaaatcca gacccgcgtc gaagttacca
    27181 ttacgcgccg ccagtcgtgc cagaccataa tagcctccgg ctttactgtt gccgctccag
    27241 accgcacgcc agaaatcctc ttcggcttgt tgatattgtc cctgacgttc gtaagcactg
    27301 gcgcgaatca aactggcctg tccgcactgc ggatttttgt tcagcgcatg tgcgcgtttc
    27361 agcgcctgac tggcatacgc caccgcttgc gggaaatctg cgcggttata ttccagcatc
    27421 gccagcgcca gattacagcg gtaatccagc gaatccagcg ccacgccgcg caggtagtaa
    27481 tcgaacggtg aacggctggc gtgatgatat tgctccagat gctgaccgat aaaccaggct
    27541 tcatctgtac tggtaatgtc ttgtgctgac agtggcgctt tggcgacgtc cggcaacggc
    27601 aactcttgcg gctgatgttc ctgataactc agtacaatat tgccgtcggc atcggagagc
    27661 tcaatggtca gcctttcagg attgataccg tgcaacacgc cctggatggc ggtcgctggt
    27721 gtcagggcca cggcatcatc aagtaacgcg ttgcatttgc cgatttcgcg gatcgccagg
    27781 cggtatccgt tcaacggaga gatggcatac agcccccact caatcccccg ctcactacgc
    27841 tggagtttta tcaccgcatc gcgggaggca ttttgcacca tgcccagaga atgataaggc
    27901 aggaaatact gctcaaaacg cttctcttcg taagcatcaa gccaggtaaa atcgggctgg
    27961 ttatcggcaa aaataccggt catcagttcg atatacgggc cgttattatc agttaggctc
    28021 ttatcccacg cctggccaaa ttcactgtgt ccccagctcc actgcttttt acctggcgca
    28081 atatggtggt tggcaacgtg tagcaaaccg ccatcttcat cgtgacacca cgcgccaaca
    28141 aaatcgtact gcgatttttc ggccatatat gaggttggaa cgggcacatt tttatagcga
    28201 gaaatgtcca ctccagcgga gtagtccact ttgtagtaag tgccggtggc gatggggaaa
    28261 gcggagacgg cccgtttgcc gtgatcaaac accgcagtca catccggcgg gaaaacgctc
    28321 tgatgccctt cccccccttt cactgccggg ttggcccacc acaagaaatg acgcggcgtg
    28381 gcgttgccgt tatagacgcg gctggcgatt tccagcgccg cccggtcagg gcgcagggtg
    28441 aaacctgtca tcacctgtaa accatgcatc ggctccgttt cgcctaccca caccgtctgt
    28501 gcgccgtctt catgggcttc gagggtgaaa tcaacgggca taaaggtggt cgggcgatgg
    28561 tgttgcggcc agttaaactc aatcccgccg gagatccacg gtcccagcag ccccaccagc
    28621 gcaggtttaa tgacttcatt gtgatagaca aaatcgcgtt gtttcacttt atcccatgcg
    28681 cgatgcaccc gaccgcccaa ttccggcagg atcatcactt tgatgtagtc gttttccagc
    28741 cacaccgcct gccaggattt cagggttttc tgctcgctca gcgtatcggt cacgccgtag
    28801 ggataaaccg cgccggacga tccctgataa acgcgatttt ccaggaacat gggatgtata
    28861 tcctgcggcc cggtttcata ggtcggtatc tcaacgcgct cttgccacac ttttactgga
    28921 gtcatggtgc cctcaatatt aacaagacat actgaattaa aagatttgtg gcagtgtatt
    28981 gaacaatctg gcaatgtttt cgcggaataa tcacgcaatt aactaaacaa ggtttagtga
    29041 agatgagagc ctgcattaac aaccaacaga ttcgccacca taacaaatgc gtgattctgg
    29101 aactgctgta ccggcaaaag cgcgccaata aatcaacgct ggcccggctg gcgcaaattt
    29161 cgattccggc agtcagtaat attttgcagg aactggaaag cgaaaaacgg gtggtgaata
    29221 tcgacgatga aagccagacg cgcgggcata gtagcggtac atggctgatt gcgccggaag
    29281 gtgactggac gctgtgtctg aacgtgacgc ccaccagtat tgagtgtcag gttgctaatg
    29341 cttgtttaag tccgaaaggt gaatttgagt atttacagat tgatgcaccg acgccgcagg
    29401 cgctgctgtc cgaaatcgaa aaatgctggc atcgccaccg taaattgtgg ccggaccgca
    29461 ccatcaatct ggcgctggca atccacggtc aggttgatcc agtgacagga gtgtcgcaaa
    29521 ccatgccgca agcgccgtgg acaacgccgg ttgaggtgaa gtatctgctg gaagagaagc
    29581 tcggcattcg ggtgatggtc gataatgact gcgtgatgct ggcgctggcg gaaaaatggc
    29641 aaaataattc gcaggaacgg gatttctgcg tgatcaacgt tgattacggc attggctcgt
    29701 cgttcgtgat taacgagcaa atttatcgcg gcagtttgta tggtagcgga cagattggtc
    29761 acaccatcgt taatccggat ggcgtcgtct gcgactgtgg acgttacggc tgcctggaaa
    29821 ccgtcgcctc gttaagtgct ttaaaaaaac aggcgcgggt atggctaaaa tcacaaccgg
    29881 ttaatactca gcttgatcct gaaaaactga caaccgcgca gttaatcgct gcctggcaga
    29941 gtggagaacc gtggatcacc agctgggttg accgcagtgc caatgccatt ggtttgagtc
    30001 tgtataactt cctcaacatc ctcaatatta atcagatttg gttgtacggt cgcagttgtg
    30061 cctttggtga gaactggctt aatactatta ttcgccagac aggatttaac ccgttcgacc
    30121 gcgacgaagg accgagcgtg aaagcgacgc aaattggctt cgggcaatta agccgcgcac
    30181 aacaggtgct gggaattggc tatttgtatg ttgaggcgca gttacgacag atttgatggc
    30241 gcgataacgt agaaaggctt cccgaaggaa gcctttttca acagattaac cgtttcgctt
    30301 atgcgtaaac cgggtaacgt gcgcagatgt cgagaacttt acctttgatg cgctcgataa
    30361 cggcttcatc attgatgctg tccagcacgt cacacatcca gccagccagt tctttcgctt
    30421 ctgcttcttt gaagccgcga cgcgtaatcg ccggagtacc cacgcgaata ccggaggtca
    30481 caaacgggct cttcggatcg ttcggtacgc tgtttttgtt gacggtgatg ttagcacggc
    30541 ccagagcggc gtctgcttct ttaccggtca ggtttttatc gaccagatca accaggaaca
    30601 gatggttatc agtaccgccg gaaaccactt tgtagccgcg ttccaggaac acttctacca
    30661 tcgctttagc gtttttagcg acctgctgct ggtaagtttt gaactcaggc tccatcgctt
    30721 ctttcagagc aaccgcttta ccagcgatta cgtgcatcaa cggaccgccc tgaccaccag
    30781 ggaaaacggc agagttcagt tttttgtaca gctcttcgct accacctttc gccaggatca
    30841 gaccgccgcg cggacccgcc agggttttgt gagtggtggt agtaacaacg tgagcatgag
    30901 gaaccgggtt cgggtagacg ccagcagcaa ccaggcccgc aacgtgcgcc atatcaacga
    30961 acaggtaagc accgatgctg tcagcgattt cacgcatttt cgcccagtca accacgccgg
    31021 aatatgcaga gaaaccaccg ataatcattt tcggcttgtg ttctttggct tgtttttcca
    31081 gatcggcgta gtcgatatga ccggtagcat cgataccgta aggaacgatg ttgtacagtt
    31141 taccggagaa gttaaccgga gaaccgtgag tcaggtgacc gccatgcgcc aggttcatac
    31201 ccagaacggt atcacctggt tccagcagcg cggtgtacac cgcaaagtta gcctgggagc
    31261 cggagtgcgg ctggacgtta gcgtagtcag cgccgaacag ttctttcgca cgatcgatcg
    31321 ccagttgttc aacgatatca acatactcgc aaccgccgta gtagcgtttg cccggataac
    31381 cttcagcata tttgttggtc agctgagaac cctgcgcctg cattacgcgc gggctggtgt
    31441 agttttcgga ggcgatcagt tcgatgtgct cttcctgacg tactttttcc tgctccatag
    31501 cctgccacag ttcggcatca taatcggcaa tgttcatttc acgctttaac atccgcatct
    31561 cctgactcag ctaacaataa aatttttggc ctttataggc ggtcctgttg gacaacggcg
    31621 aacagtataa ccgaatcatt gtgcgataac aggtcttgac aaaggaattt acgcaaacga
    31681 ttaccttcag gctacgcaag gctttggaga ataaagagct tgcaaccgga aacggatttc
    31741 ttttcaggtt tgtgatgcaa atttttcact tcatcacatt ctttctgaaa aacaccaaag
    31801 aaccatttac attgcagggc tattttttat aagatgcatt tgagatacat caattaagat
    31861 gcaaaaaaag gaagaccata tgcttgacgc tcaaaccatc gctacagtaa aagccaccat
    31921 ccctttactg gtggaaacgg ggccaaagtt aaccgcccat ttctacgacc gtatgtttac
    31981 tcataaccca gaactcaaag aaatttttaa catgagtaac cagcgtaatg gcgatcaacg
    32041 tgaagccctg tttaacgcta ttgccgccta cgccagtaat attgaaaacc tgcctgcgct
    32101 gctgccagcg gtagaaaaaa tcgcgcagaa gcacaccagt ttccagatca aaccggaaca
    32161 gtacaacatc gtcggtgaac acctgttggc aacgctggac gaaatgttca gcccggggca
    32221 ggaagtgctg gacgcgtggg gtaaagccta tggtgtactg gctaatgtat ttatcaatcg
    32281 cgaggcggaa atctataacg aaaacgccag caaagccggt ggttgggaag gtactcgcga
    32341 tttccgcatt gtggctaaaa caccgcgcag cgcgcttatc accagcttcg aactggagcc
    32401 ggtcgacggt ggcgcagtgg cagaataccg tccggggcaa tatctcggcg tctggctgaa
    32461 gccggaaggt ttcccgcatc aggaaattcg tcagtactct ttgactcgca aaccggatgg
    32521 caaaggctat cgtattgcgg tgaaacgcga agagggtggg caggtatcca actggttgca
    32581 caatcacgcc aatgttggcg atgtcgtgaa actggtcgct ccggcaggtg atttctttat
    32641 ggctgtcgca gatgacacac cagtgacgtt aatctctgcc ggtgttggtc aaacgccaat
    32701 gctggcaatg ctcgacacgc tggcaaaagc aggccacaca gcacaagtga actggttcca
    32761 tgcggcagaa aatggcgatg ttcacgcctt tgccgatgaa gttaaggaac tggggcagtc
    32821 actgccgcgc tttaccgcgc acacctggta tcgtcagccg agcgaagccg atcgcgctaa
    32881 aggtcagttt gatagcgaag gtctgatgga tttgagcaaa ctggaaggtg cgttcagcga
    32941 tccgacaatg cagttctatc tctgcggccc ggttggcttc atgcagtttg ccgcgaaaca
    33001 gttagtggat ctgggcgtga agcaggaaaa cattcattac gaatgctttg gcccgcataa
    33061 ggtgctgtga tttgatgttg ccggatggaa acatccggca acccttgacg cggtttaaat
    33121 tgccgcgtcg tcctcttcac cggtacggat gcgaatgacc cgtgccacgt caaagacgaa
    33181 gattttaccg tcaccgattt tgccggtttg cgcagtgcga ataatggtat cgacacaggt
    33241 atcgacaatg tcgtccggta cgacaatctc aattttcact ttcggcagaa aatccaccat
    33301 atactccgcg ccgcggtaca gctcggtatg gcctttctgg cggccaaaac ctttcacctc
    33361 agtgaccgtc atgccggtaa tgccgacttc agccagtgct tcgcggacat cgtccagctt
    33421 gaagggttta ataatcgcat caatcttttt catgctattc cttgaaaagg tcgcctgtct
    33481 tttgatctgc taaacgtaac acataacgcc aattcattcc ttgaaatcgt ttgcatccag
    33541 ctcgtgtcgg gaaagcagtt tataaaattc tgtccggttg cgccccgcca ttctcgccgc
    33601 atgggtgacg ttgcctttgg taatttgcag cagcttacgc aaatagttga gttcaaactg
    33661 attacgtgcc tcaacaaagg ttggcagcgc cgtgttttcg ccctccagcg cctgctctac
    33721 taatgcatca ctaatcaccg gagatgaggt cagcgccacg cactgttcaa tcacgttgac
    33781 caactggcgc acattacccg gccagctcgc ggtcatcagg cgtttcatcg catcggtaga
    33841 gaacgcgcgg acaaacggtt tatgtcgctc tgccgcctgg cgcaacaggt gatttgccag
    33901 tagcggaatg tcttctgtgc gctctgccag agccggaatt ttcaggctga caacgttaag
    33961 gcggtaatag agatcttcac ggaattcccc gcgcaccatc gcttttggca ggtcgcggtg
    34021 agtggcggaa ataatccgca cgtcgatatc aatatcgcgg ttactgccca gcgggcgcac
    34081 tttacgctcc tgcaacacgc gcagcagttt gacctgcaac ggtgcgggca tatcgccaat
    34141 ctcatcgaga aatagcgtac cgccttccgc cgcctggaat aaaccttcgc gattgctgac
    34201 agcgccagta aacgcgccac gcgcatgacc aaacagctcc gactccagca attgctcggg
    34261 caatgcgcca cagttaatag caataaatgg tttgctattg cgcgggctgg cgttgtggat
    34321 agcctgggcg aacatctctt tcccggtgcc gctctgaccg ttaatcaaaa cgctaacgtc
    34381 tgattgcgcc accagccgcg cctgttccag caaacgcagc atcagcgggc tgcgggtgac
    34441 aatcgcctcg cgccagcgtt cgtcggtggc tggcgcggat tgctccagcg catcatcaat
    34501 tgcctgatat agcgcgtctt tatcgacagg cttggtgagg aaactaaaaa cgccctgctg
    34561 tgttgcagca acggcatcgg gaatagaacc atgcgcggta agaataatta ccggcattcc
    34621 cggctgcact ttctggattt cagcaaacag ctgcataccg tccatttcat ccatccgcag
    34681 gtcgctgata actaaatcta ctttttcgcg attcagtacc cgtaaacctt cagcgccact
    34741 ttccgccgtg accacactgt agccttcgct ggtcaggcgc aggccaagca gtttcagcaa
    34801 tcccggatcg tcatcgacca ataataaatg cgcaggttta tggctcatca ggagtgacct
    34861 catgggtgga tggcgcgggc ttttcactct cgtgtggcgt atccggcgag aaatttccag
    34921 ccggtttgcg ggtcgagagc tggcgttcaa tatcagtcag gttttccagc ttgcgggtgg
    34981 tgagttccag ttgctgctgt agaacgtgat gttgctggcg cagagcgtcc agctcgctgt
    35041 cgctggactg ctggagttta ctgtagcgtt ggcgctcttc cgccagttgc agttgcagcg
    35101 cctgaccgtc gcgccagagt tgatacagtg ggcgaacctg tgccgggatc tcggtactta
    35161 acgcttcaat acgcgcgacc agctggcggc gctcatacgg cgtaattttg gcgtcggcga
    35221 gcaaaatccc ttgtttaaag gtattttgcc agctgccgtc gtcatattgg cgggcttgct
    35281 gacgcgactg tgcaggcatt aaacgatcag cacaatccat cgcccgcagc cagtaaagcg
    35341 gattggtttc agttgattta ccttgcagcg cccagatgtc gctacattca gtagaaagat
    35401 agtcagccag ttgataaacc ggaatttttt cttctgctgg cgtatcaatg gctggcttat
    35461 tgtgactctg cacgcaaccc agcaatgcca gacatggcag ccctgccagc cacagccgtc
    35521 ggggcaataa tcgttgaaaa atgtgtcgca tattcaccag acttaaagcc tatcccagtg
    35581 ggcgtaattg ttgcagacag tctggacatg gacagcgcgg agaaaccgga gcgtacatgt
    35641 cgtacgtgag gatttcgagc actgcccggg gccgaactga caaataaaat agcctgatgg
    35701 gatagactgt tagcattatt tcgtgttttt cgacgacggt aattcaatgc ggaaacaaac
    35761 gtcttgcccg ctctcgtcga ccagatacag ttccccttgc atacggcgaa tacaatccct
    35821 ggcaatgctt aatcccagac cgctgccctt caccgcccct tttcgctggt ggcttccctg
    35881 aaaaaagggt tcgaagatca tggcgcgttc ctcttgcgga atgggcgtgc ctgtattgat
    35941 gacatcaata taaacccgcg cgccatgtaa actgctgcga aggcaaatgt taccggattc
    36001 agccccgtag tgcaccgcat tggagtaaag attatccagt acgctcatca gcagcattgg
    36061 ctccgccagg caagctgttg ctttgagatc gacgtcggta tgcatcattt tagcccgtgc
    36121 gggcaggcta tgagcagaaa ccactgtctc caccagcggt gctaattcaa cattctccag
    36181 ttccaccgca ctgtccgcct gtttacggtt gtaatcaagc agttgttcga tcagtttttg
    36241 caaattgcgg ctgctgctat caagaatgct caccacctct ttttgctctg gcgtaagcgg
    36301 cccgacaacc tgatcagcca gtaattcagt gccctcgcgc atactcgcca gtggcgtttt
    36361 taattcatga gataaatgtc ttaaaaattg atggcgttgg gattccagcc atgacaggcg
    36421 ctcacttaac caaagaatac gttgcccaac cgagcgtaac tcgctcggtc cactgaacga
    36481 aacgctattg cccagagaac gcccttcccc caaccgattg atcatccgtt caatattttt
    36541 caccggcccg ataatcatcc gcgtgaaaag tagcaccatc accagactca ccagaaatag
    36601 caccagcgat tgccaaccaa aatattgccc acgttcggcg atttcacgct gaagttgctg
    36661 cccacgagag aacaccactg tgcgcgtggc ctgcaccatt tcggtattgg cactggcaaa
    36721 ggcttcaaga cgcgcggcgg cggcagcatc gggaccactg ttgttgcact gaagttgggc
    36781 cagattgttc aagtcctgac gcaatgcctg gtaaagttta tcgtctggca gtacgcctgc
    36841 gtgggcatcg agcatttcgc tgtaacgctt gcgctggctt tgataaacct tcgcaagcgt
    36901 agggtcatcc agcacgcaat actgacggta gctacgctcc atctccagcg ccgcgttggt
    36961 cattgcttcg ctgcgccggg catcgataag cgtggtgcgg ttgaccagcg ccgcctggtc
    37021 gctaagcgca ttcaggcttt gccaggcttg ccatgccagc accaacaggg gcagcagaat
    37081 cagcaaaaat gccagcatta ccagttgtcg taatgagcgg ggaaaaacgg gccagcgttt
    37141 caaggtgtta ctctcgtcag acgcgaatag cctgatgcta accgagggga agttcagata
    37201 caacaaagcc gggaattacc cggctttgtt atggaataag gcggtgccta actcgacgtt
    37261 tcgcccgatg gttgatatag ctacgctgat atcagaagtt ggacggcagg caccttgttg
    37321 tgcgtcattc gaattttatg tagcacgtcc cgaaggggct gacataagtc ggtgaatgag
    37381 ccactgatta ctattatgca gtaactgtgc caataaagaa aatagtttgg taacgtactg
    37441 attatacgtt gctttgaggg gtttatggct tccccgctat ttgaatgtaa atcagccact
    37501 gtgtctctaa aaagagacaa cttaagaaaa acttattaga ttacaataaa atcaatcatt
    37561 taagtgtcgc cagaaagcga cacggtaaac cacccattgt cgtgatttac agacacaaaa
    37621 aagctcccga agttgggagc ttataatagc ggttgtattt aatgccggat gcggcgtgaa
    37681 tgcctcatcc gggctgcaaa accaatgagt ttgacggctt accccaactg cttacgtgca
    37741 ttgcggaaaa tgcgcatcca tgggccatcc tcgccccagt tttcaggatg ccaggagttg
    37801 ctgacagtac ggaaaacacg ttccgggtgc ggcatcataa tggtgactcg accgctttca
    37861 gtcgtgaccg ccgtaatacc gttcggagaa ccgttcgggt tagccgggta ggtttcagtg
    37921 actttgccga agttatcgac atagcgcagt gccaccagcc ctttgctttc cagtgccgcc
    37981 agatgcgccg catcacgcac ttccacgcgc ccttcaccat gagagacagc aatcggcatt
    38041 tgcgagccca ccatcccctg caacagcagc gacgggcttt gggtcacttc aaccaggctg
    38101 aaacgcgctt caaagcgatc ggaggtattg cgcacaaaac gtggccacaa ctcactacct
    38161 gggatcagtt cacgcagatt agacatcatc tggcaaccgt tacatacccc cagcgccagc
    38221 gtttgcggac ggtggaagaa ggttgcaaac tcatcgcgta cacggtcatt gaacaggata
    38281 gacttcgccc aaccttcacc ggcacccagc acatcaccgt aggagaaacc accgcacgcg
    38341 accagggcgt ggaaatcctc caggcccgtg cgtccagcca gcaggtcgct catatgcacg
    38401 tcgatagcat cgaagcctgc acggtggaaa gctgccgcca tttcaacatg cgagttcacg
    38461 ccctgctcac gcagcacagc aactttcgga cgtgcgccag tggcaatata cggtgctgcc
    38521 acatcttcgt tgatatcgaa cgacagtttt acattcaggc ccggatcggc gtcgtttgat
    38581 ttcgcctgat gctcctgatc ggcacactcc gggttgtcac gcaggcgctg catctgccag
    38641 gtagtttctg cccaccagac acgcaacgta gtgcggcttt cgctgaatac agtctgcccg
    38701 ttggcggtaa tcacaaaacg gtcaccggaa accgcctgcc caacataatg gacgcaatca
    38761 gcaagcccat gctgtgccag tacggactcg accgcttcac ggtcagcggc acgaacctga
    38821 atgaccgcac ccagttcttc gttaaacaac gccgccaggc gatcgtcacc cagagtggcg
    38881 atatccgcgt aaatgccgca atgaccagca aaggccattt ccgccagcgt taccagcaga
    38941 ccgccatcag agcggtcgtg atacgccagc agcttacgct gtgcaaccag cgcctgaatc
    39001 gcgtcataga agcctttcag ttgcgcgaca tcgcgtacat ctgccggttt gtcgccaagc
    39061 tgacgataaa cctgcgccag cgccgttgcg cccagcgcgt tattgccttt gcccaaatca
    39121 atcagcagca gtgcgttatc ttcggtagaa agctgcggcg tgatggtgtg acgtacatct
    39181 tccacgcggg caaatgcaga aatcaccagc gacagcggcg acgtcatttc gcgctcttcg
    39241 ttaccttcct gccagcgggt tttcatcgac atagagtctt tacccaccgg gatcgtcagg
    39301 cccagcgccg gacaaagctc ttcgcccacg gctttaacgg cttcatacag gcccgcatct
    39361 tcaccagggt ggcctgccgc cgccatccag ttggcggaaa gtttaatgcg tttgatatcg
    39421 ccaatctgtg ttgcggcaat attggttaac gcttcaccga ccgccagacg ggcagaggcg
    39481 gcgaaatcca gcagcgcaac cggcgcacgc tcgccaatcg ccatcgcttc accgtagtag
    39541 ctgtcgaggc tggcggtagt gaccgcgcag ttagcgaccg gcacctgcca cggccccacc
    39601 atctgatcgc gcgccaccat gccggttacg ctgcggtcac cgatggtcac caggaaagtt
    39661 ttctccgcca cggtcggcag atgcagtacg cgtttcaccg cgtctgcaat ggtgatccct
    39721 tcacggacca gcgcatcgcc cttcgctttc agcgtttgta catcgcgggt catcttcggc
    39781 gttttaccaa gcaggacgtc cagcggcaga tcgatcggct gattatcaaa atgacgatcg
    39841 tgcagagaaa gatgcagttc ttcggtcgct tcaccaatca ccgcgtaggg tgcgcgctca
    39901 cgcttacaca gttcgtcaaa cagcggtaac tgatcggcag caaccgccag cacgtagcgt
    39961 tcctgggatt cgttacacca gatttccagc gggctcatgc ccggttcgtc gctcaggata
    40021 tcgcgcagtt caaatttacc gccgcgcccg ccgtcgctta ccagttccgg catcgcgtta
    40081 gaaagaccgc ctgcaccaac gtcgtggata aacaggattg ggttggcatc accaagctgc
    40141 cagcaacgat cgatcacttc ctggcagcga cgctccattt ccgggttgtc gcgctggacg
    40201 gaagcaaagt cgaggtcggc atcagactga cctgacgcca tggaagacgc cgctccgccg
    40261 ccaagaccga tgttcattgc cgggccgccg agaacgacca gcttcgcacc gacgttgatc
    40321 tcgccttttt gtacgtgatc ggcgcgaatg ttgccgatcc cgcccgccag catgatcggt
    40381 ttgtgataac cgcgcagctc ttcgccgttg tggctgttca ctttttcttc ataagtacgg
    40441 aagtagccat tcagtgccgg acgaccaaat tcgttgttaa acgccgcgcc gcccagcggg
    40501 ccttcggtca tgatgtccag cgcggtgaca atgcgctcag gcttaccgaa atcttcttcc
    40561 cacggctgtt cgaagcccgg aatacgcagg ttggaaacgg agaaaccgac cagaccagct
    40621 ttcggcttcg cgccacgccc ggtggcgcct tcatcgcgga tttcaccgcc ggaaccggtc
    40681 gccgcgcccg gccacggtga aatcgccgtc gggtggttgt gggtttcaac tttcatcagg
    40741 atatgcaccg gttcctgatg gaaatcgtag cggcccgttt cgtggtcggc aaaataacgg
    40801 cccacttcag aaccttccat tacggcggcg ttatctttat aagcagagag aacgtgatct
    40861 ggcgtggttt cgaaagtatt tttgatcatc ctgaacagcg atttcggctg ctgttcaccg
    40921 tcgataatcc agtcggcatt aaaaattttg tggcggcagt gctcagagtt cgcctgagcg
    40981 aacatataca gctcgatgtc gttcgggtta cgaccaagct ttgtgaaagc atcctgcaga
    41041 tagtcaattt catcttccgc cagagccagg ccaagacgca ggttagcgtc gatcagcgcc
    41101 tgacggccct gccccagcaa atcaacgctg gtaaccggag tcggttgatg gtgggcaaac
    41161 aactgctctg catcatctaa agcaaaaaag accgtttcca tcatgcggtc gtgcagttca
    41221 gcggtaacct gctgccattg ttcattggtc agcgtaccgg cttctatata gtaagcaacg
    41281 ccgcgctcaa ggcggtttac ctgttgtagc ccgcagttat gggcaatatc ggtcgctttc
    41341 gaagaccagg gagagatggt gccaggacgc ggggtcacca gcaggagttt gccttgcggg
    41401 gcgtggctgg cgagtgccgg gccatatttc agcaggcgtt caagttgtgc gtgctcatca
    41461 tcgtttaacg gcgcattgag gtcagcaaaa tggacatact cggcgtaaat attgtgaacc
    41521 gggagcctgg cagcctgaaa acgtgccagc agtttgttga ttcggaatgc cgacagtgca
    41581 ggcgaaccac gcagaatttc catcataagt ctctcgtctt ctaagctttc ggtgtaccca
    41641 agggggggaa acgggcgtca ttataaagaa tctgatgcgc tgacgaaacc gtttgcgtgg
    41701 aaataaaatc accatcgtga attagcaacg cgtgccgcca atggctgtaa taagttgcca
    41761 tctggcgcag gtttacgcaa aatgccgctc atttatgagt aaacctttca ctattattac
    41821 gttttttcaa gctgggacgc gcacgacaca gagaattaac taattgaaaa aattaaagat
    41881 taattatctg ttcatcggca ttctggcact gctgctcgcg gtcgctctct ggccatccat
    41941 tccctggttt ggtaaagccg acaaccgtat cgccgccatt caagcgcggg gagagttgcg
    42001 tgtgagcacc attcatactc ccctgactta taacgaaatc aacgggaaac cttttggcct
    42061 ggattacgaa ctggcgaaac agtttgccga ttacctcggt gtaaaactga aagtgaccgt
    42121 gcggcagaat atcagccagc tgtttgacga cctcgataat ggtaacgccg acctgctggc
    42181 ggcaggactt gtctataaca gtgagcgggt aaaaaattat cagcctggcc ctacctatta
    42241 ttccgtgtca caacaactgg tttataaagt gggtcagtat cgcccacgta cgctgggcaa
    42301 cctgacggcg gagcaactca ccgttgcacc gggtcatgtg gtggttaacg atctccagac
    42361 cctgaaagaa acaaaattcc cggaattaag ctggaaggta gacgacaaaa aaggctctgc
    42421 ggaattaatg gaagatgtca tcgaaggaaa actcgattac accattgctg attctgtcgc
    42481 catcagcctg tttcagcgcg ttcacccgga gctcgccgta gcgctcgata tcaccgatga
    42541 acaaccggtg acttggttta gcccgttaga tggcgataat accctttccg ccgccctgct
    42601 cgacttcttc aacgaaatga atgaagacgg tacgctggca cgcattgaag agaaatacct
    42661 ggggcatggc gatgattttg attacgtcga tacgcgcaca tttttacgcg ccgtcgatgc
    42721 ggtactgccg cagttaaagc ccctgtttga gaaatacgcc gaagaaattg actggcgttt
    42781 gctggccgct attgcttatc aggaatcgca ctgggatgca caggccactt caccgacggg
    42841 tgtgcgcggc atgatgatgt taaccaaaaa taccgcgcaa agcctcggca ttacggatcg
    42901 taccgatgcc gaacagagca tcagcggtgg cgtgcgttat ttgcagcata tgatgagtaa
    42961 agtgccggaa agtgtgccgg agaacgagcg gatctggttt gccctcgctg cgtacaatat
    43021 gggctatgcg catatgctgg atgcccgcgc cctgacggca aaaaccaaag ggaatcctga
    43081 cagttgggct gacgtaaaac agcgtctgcc tttacttagc cagaaaccct attacagcaa
    43141 gctgacttac ggctacgctc gtgggcatga agcctacgct tatgtcgaaa atattcgtaa
    43201 gtatcagatt agcctggtgg gttatctgca agagaaagag aagcaggcta cagaagcggc
    43261 gatgcaactg gcgcaggatt atccggcggt atcgcctacg gagttgggca aagagaaatt
    43321 tccttttctc tcgtttcttt cccagtcgtc atcaaactat ttgacccatt ctccctctct
    43381 gctgttttcc aggaaaggga gtgaagagaa acaaaattaa tccgtcgagg attgcgcttt
    43441 tttctgcgct ttaatttcct ggcggcgcat gcgaaagaag tcactgagca acgccgcgca
    43501 ctcatccgcc agtattcctt ccgtaatttc cactcggtga ttcatacccg gatgatgcag
    43561 cacatccatt aaagatcccg cagcgccagt tttcgcgtca cgcgcaccaa agaccacgcg
    43621 accaatgcga ctgtggatca tcgctccggc acacattaca catggttcaa gcgtgacata
    43681 caacgtggcg tcgatcagac gataattttg catcaccaga ccgccctgcc tcagggccat
    43741 gatttctgca tgtgcggtgg gatcatgtcg accaatcgga cggttccagc cttcgccaat
    43801 aacacgattg ttatgcacta ataccgcgcc gaccggtact tcccgctcat cccaggcacg
    43861 cttcgccagc gtcattgcat gacgcatcca gtattcgtgg ctaaattcga cttcagacaa
    43921 aaagaaaact ccggttataa aagcgcggcg cattatacac ggacgctatg ctttactcta
    43981 ttccagttgc tggagttcac cgcgcggggt tactcgccag cgatgctggc aaaagtaaag
    44041 cagcgggttg tcctgattac tgtcgctata gccactgtac agccgcagcg gcgtgccgat
    44101 tttgcgctcc agttgcgcga ccttttcatg tcccagacaa cgcatcgtca atacccaacc
    44161 accatagcca cgctgaattt ggctggcgat aagattaact cgcggcagcc agggcgtatc
    44221 gaaataaacc gcttcaacca gcggctgcgg agagccggta atcaaccaga tatcagcatc
    44281 ggaacttaac aggtaggtgg ttaatcgctc ctgaaccagc ggaaaggcgg taacattgtc
    44341 acgaaaccag cgtacgaaat cggcctgcaa cgcctgcaaa cgcgcttcat tgtgaccaaa
    44401 agtgcatccc cacagaagca gactcatcgg ccagcgtgcc gcacgacctt ttatcaataa
    44461 cgcaatggct ataatcggca acaacggcag gacaagtaac gcattcagcg gttggcgacg
    44521 cagtaaatag cgcagaaaac tgccgaacat atcctgctga tgcaatgttc catctaaatc
    44581 aaaaaacacc acacgacgct cgtgagttgc caaaccatac tcctctggac tgaaacatcc
    44641 tgattaattt tcgctactac atagcctaac agatagatca tcacttttcc ggcaacaatc
    44701 gaccgcatca tggctaactg gaatttttaa ttcatggcaa ttagcggcaa tggaatataa
    44761 aattcactcg cgtgtgtctc atattgcctg ttgtcgccac tatttcatcg cgaggaaaga
    44821 tatgaacggc ttacttcgta tccgtcagcg ttaccagggg cttgcccaga gtgataaaaa
    44881 actggcggat tatctgctgc tacaacctga tacggcgcgc catttaagct ctcagcaact
    44941 ggccaacgaa gccggagtca gtcagtccag cgtcgtgaag ttcgcgcaaa aactcggcta
    45001 taaaggtttt ccggcgctta agttggcgtt gagtgaagcg ctggcaagcc agccggaatc
    45061 gccctcagtg cccattcata accaaatccg cggtgatgat ccgttacggc tggtcggcga
    45121 aaaactgatt aaagaaaata ctgccgctat gtacgcaacg ctaaacgtta atagtgaaga
    45181 gaaactgcat gaatgcgtaa caatgttgcg ctctgcgcgg cggataattc tgaccggtat
    45241 tggcgcttcg ggtctggtgg cgcaaaactt tgcctggaag ctgatgaaga ttggcttcaa
    45301 tgctgccgca gtgcgcgata tgcatgcgct actcgctacc gtacaggcgt cgtcccctga
    45361 cgatctgtta ttagccattt cttacaccgg tgtacgacgc gagttaaacc tggcggcaga
    45421 tgagatgctg cgagtgggcg gaaaagtgct ggcgattacc ggctttactc ccaatgccct
    45481 gcaacagcgt gcttctcatt gcctgtatac cattgccgaa gaacaagcga caaacagtgc
    45541 ttcaatctct gcttgtcacg cgcagggaat gttaacggat ttgctgttca ttgcgctgat
    45601 tcagcaggat ctggaactgg caccagaacg tattcgtcat agtgaagcgc tggtgaaaaa
    45661 actggtctga gtaaagaatg cgcgtataat gcccgcccgg tttgtgttgt tttgagagtt
    45721 tccttatggc gctgttaatc actaaaaaat gcatcaattg tgatatgtgt gaaatcatat
    45781 aaaaacacat ttattttcat agtgatacag taatgtgtta ttgaatgtgt tgttggattt
    45841 atggtgcaaa aaaattttgc ataagttcag caacacaaag cctccccctt tctttagcat
    45901 tttctactga gcagccatcc gctttcagac ctcacttaaa ttacgcttat tcagagaaga
    45961 gcgtcagaaa cacgatcggc atcactgttt ctgcaaactt ccctgtggca gtctttattc
    46021 gccatataac gtagcactaa agatcttcat taatgtttcc attatagtta atacttcctt
    46081 gtaccggggg tggtgatacc cccgtttttt cattatccct gttcccttaa tcgcactgcc
    46141 aggcgccata aaacacatca actgtggctg gtatggaaac accaaaactc tcacttccgg
    46201 aaatctgaaa aattatccat atcaacagga tgctctggaa atgagaccgg tatcactttc
    46261 aggattaact tttccgtggc actctgcatc cgtgtaggta aagtctcttc acaacaatta
    46321 gataatcgac tcagttcatg ggggaggtga tgg
//
LOCUS       DAZFKF010000031        43608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFKF010000031 DAZFKF010000000
VERSION     DAZFKF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.031"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            35..1084
                     /gene="yeiH"
                     /locus_tag="ACHKA1_002121"
     CDS             35..1084
                     /gene="yeiH"
                     /locus_tag="ACHKA1_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO5679775.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1158..2015
                     /gene="nfo"
                     /locus_tag="ACHKA1_002122"
     CDS             1158..2015
                     /gene="nfo"
                     /locus_tag="ACHKA1_002122"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO5679776.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            2018..3106
                     /gene="yeiI"
                     /locus_tag="ACHKA1_002123"
     CDS             2018..3106
                     /gene="yeiI"
                     /locus_tag="ACHKA1_002123"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5679777.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3162..4412)
                     /locus_tag="ACHKA1_002124"
     CDS             complement(3162..4412)
                     /locus_tag="ACHKA1_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO5679778.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     MLSPATESSQVSFNNLSFTETPPKSIIEAAATGAVTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWTDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(4512..5453)
                     /gene="rihB"
                     /locus_tag="ACHKA1_002125"
     CDS             complement(4512..5453)
                     /gene="rihB"
                     /locus_tag="ACHKA1_002125"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO5679779.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5583..6281
                     /gene="yeiL"
                     /locus_tag="ACHKA1_002126"
     CDS             5583..6281
                     /gene="yeiL"
                     /locus_tag="ACHKA1_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO5679780.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(6349..7599)
                     /gene="psuT"
                     /locus_tag="ACHKA1_002127"
     CDS             complement(6349..7599)
                     /gene="psuT"
                     /locus_tag="ACHKA1_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO5679781.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFTNASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(7693..8631)
                     /gene="psuG"
                     /locus_tag="ACHKA1_002128"
     CDS             complement(7693..8631)
                     /gene="psuG"
                     /locus_tag="ACHKA1_002128"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO5679782.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(8619..9560)
                     /gene="psuK"
                     /locus_tag="ACHKA1_002129"
     CDS             complement(8619..9560)
                     /gene="psuK"
                     /locus_tag="ACHKA1_002129"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO5679783.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQSAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNLIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(9984..11675)
                     /gene="fruA"
                     /locus_tag="ACHKA1_002130"
     CDS             complement(9984..11675)
                     /gene="fruA"
                     /locus_tag="ACHKA1_002130"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO5679784.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVASLAYAFLKRPEVDAVAKAA"
     gene            complement(11692..12630)
                     /gene="fruK"
                     /locus_tag="ACHKA1_002131"
     CDS             complement(11692..12630)
                     /gene="fruK"
                     /locus_tag="ACHKA1_002131"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO5679785.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(12630..13760)
                     /gene="fruB"
                     /locus_tag="ACHKA1_002132"
     CDS             complement(12630..13760)
                     /gene="fruB"
                     /locus_tag="ACHKA1_002132"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO5679786.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            14128..15309
                     /gene="setB"
                     /locus_tag="ACHKA1_002133"
     CDS             14128..15309
                     /gene="setB"
                     /locus_tag="ACHKA1_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO5679787.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(15306..15560)
                     /gene="yeiW"
                     /locus_tag="ACHKA1_002134"
     CDS             complement(15306..15560)
                     /gene="yeiW"
                     /locus_tag="ACHKA1_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO5679788.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            15715..16287
                     /gene="yeiP"
                     /locus_tag="ACHKA1_002135"
     CDS             15715..16287
                     /gene="yeiP"
                     /locus_tag="ACHKA1_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO5679789.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            16510..17976
                     /gene="yeiQ"
                     /locus_tag="ACHKA1_002136"
     CDS             16510..17976
                     /gene="yeiQ"
                     /locus_tag="ACHKA1_002136"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO5679790.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQSGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRDLVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            18094..19080
                     /gene="yeiR"
                     /locus_tag="ACHKA1_002137"
     CDS             18094..19080
                     /gene="yeiR"
                     /locus_tag="ACHKA1_002137"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO5679791.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNGLQSALLKLRLATTA"
     gene            19119..19832
                     /gene="lpxT"
                     /locus_tag="ACHKA1_002138"
     CDS             19119..19832
                     /gene="lpxT"
                     /locus_tag="ACHKA1_002138"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO5679792.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFSDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            20244..20810
                     /gene="mepS"
                     /locus_tag="ACHKA1_002139"
     CDS             20244..20810
                     /gene="mepS"
                     /locus_tag="ACHKA1_002139"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO5679793.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            20991..22547
                     /gene="pdeN"
                     /locus_tag="ACHKA1_002140"
     CDS             20991..22547
                     /gene="pdeN"
                     /locus_tag="ACHKA1_002140"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO5679794.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGIEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            22629..24443
                     /gene="yejA"
                     /locus_tag="ACHKA1_002141"
     CDS             22629..24443
                     /gene="yejA"
                     /locus_tag="ACHKA1_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO5679795.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSAKQQGE"
     gene            24444..25538
                     /gene="yejB"
                     /locus_tag="ACHKA1_002142"
     CDS             24444..25538
                     /gene="yejB"
                     /locus_tag="ACHKA1_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO5679796.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPLHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            25538..26563
                     /gene="yejE"
                     /locus_tag="ACHKA1_002143"
     CDS             25538..26563
                     /gene="yejE"
                     /locus_tag="ACHKA1_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5679797.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            26565..28154
                     /gene="yejF"
                     /locus_tag="ACHKA1_002144"
     CDS             26565..28154
                     /gene="yejF"
                     /locus_tag="ACHKA1_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO5679798.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(28158..28502)
                     /gene="yejG"
                     /locus_tag="ACHKA1_002145"
     CDS             complement(28158..28502)
                     /gene="yejG"
                     /locus_tag="ACHKA1_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO5679799.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(28835..30025)
                     /gene="bcr"
                     /locus_tag="ACHKA1_002146"
     CDS             complement(28835..30025)
                     /gene="bcr"
                     /locus_tag="ACHKA1_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO5679800.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(30053..30748)
                     /gene="rsuA"
                     /locus_tag="ACHKA1_002147"
     CDS             complement(30053..30748)
                     /gene="rsuA"
                     /locus_tag="ACHKA1_002147"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO5679801.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            30897..32657
                     /gene="radD"
                     /locus_tag="ACHKA1_002148"
     CDS             30897..32657
                     /gene="radD"
                     /locus_tag="ACHKA1_002148"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO5679802.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAELRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            32782..33066
                     /gene="rplY"
                     /locus_tag="ACHKA1_002149"
     CDS             32782..33066
                     /gene="rplY"
                     /locus_tag="ACHKA1_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO5679803.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(33205..34212)
                     /gene="yejK"
                     /locus_tag="ACHKA1_002150"
     CDS             complement(33205..34212)
                     /gene="yejK"
                     /locus_tag="ACHKA1_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO5679804.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVCQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            34394..34621
                     /gene="yejL"
                     /locus_tag="ACHKA1_002151"
     CDS             34394..34621
                     /gene="yejL"
                     /locus_tag="ACHKA1_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO5679805.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            34641..36401
                     /gene="yejM"
                     /locus_tag="ACHKA1_002152"
     CDS             34641..36401
                     /gene="yejM"
                     /locus_tag="ACHKA1_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO5679806.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFITFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            36476..36552
                     /locus_tag="ACHKA1_002153"
     tRNA            36476..36552
                     /locus_tag="ACHKA1_002153"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36510..36512,aa:Pro,seq:ggg)
     gene            36771..38012
                     /locus_tag="ACHKA1_002154"
     CDS             36771..38012
                     /locus_tag="ACHKA1_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO5679807.1"
                     /translation="MARKTHPLTTVQIKAARPAQKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLEDVSLADARKRRSEYRTLISAGTDPQDHERQKRETEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLEKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRINEVMDYAANSGLIDTNPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPVEASSARWEEIDLDAQQWTIPAGRMK
                     MRRDHVIPLCSQAMAVLEAMKPISHHRDYVFPSLKDPRQPMNSQTANAALRRMGFGGI
                     LVSHGMRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            38111..38341
                     /locus_tag="ACHKA1_002155"
     CDS             38111..38341
                     /locus_tag="ACHKA1_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679808.1"
                     /translation="MQKNIRCNCDGLQLALMVQHEFWSTYDPEDRTTAPSKKQVVDFL
                     VSRGASRNLAVSIDRVVRPASMKTGGRPRKWR"
     gene            38510..38716
                     /locus_tag="ACHKA1_002156"
     CDS             38510..38716
                     /locus_tag="ACHKA1_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5679809.1"
                     /translation="MEAIKKVIFRQEVKKLIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVIAWMKEREQEARVTAA"
     gene            complement(38859..39035)
                     /locus_tag="ACHKA1_002157"
     CDS             complement(38859..39035)
                     /locus_tag="ACHKA1_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679810.1"
                     /translation="MTNEESTKKTSPARKRRRRKKEHESERFAPCSFALEKYLKEYSS
                     KERASQLWKRTETD"
     gene            complement(39093..39272)
                     /locus_tag="ACHKA1_002158"
     CDS             complement(39093..39272)
                     /locus_tag="ACHKA1_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679811.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            39402..39599
                     /locus_tag="ACHKA1_002159"
     CDS             39402..39599
                     /locus_tag="ACHKA1_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5679812.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGSHHA"
     gene            39592..40059
                     /locus_tag="ACHKA1_002160"
     CDS             39592..40059
                     /locus_tag="ACHKA1_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5679813.1"
                     /translation="MRKNRLQKIITGLYASPVAHMATGFDSLNTSGGQPPISDKRFFC
                     VRKPTHTRIMAGRNGGAFALAGFQMTSLSTLLRLATMFDSVVARLLKFHLGAFHMAVF
                     ARPYFVWRFMQCHHSSIDIYTVTAATEREARARLPHAHLIFVARIRQGKTYAQ"
     gene            40049..40270
                     /locus_tag="ACHKA1_002161"
     CDS             40049..40270
                     /locus_tag="ACHKA1_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679814.1"
                     /translation="MHNKTTPDAAAAALTTLMHALIDIECTAGLAQKEEREEYTLFAL
                     ECIRYTATRSLNDAKNILIADCENGGGYA"
     gene            40263..40412
                     /locus_tag="ACHKA1_002162"
     CDS             40263..40412
                     /locus_tag="ACHKA1_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679815.1"
                     /translation="MRDDRFNSLKQEFSGVSDDAADALSAISELIRAAFFLLGTKENK
                     TTDGN"
     gene            40414..40647
                     /locus_tag="ACHKA1_002163"
     CDS             40414..40647
                     /locus_tag="ACHKA1_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679816.1"
                     /translation="MRDIYLETIDRAFLALSHSEIMMEMLRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            40653..40952
                     /locus_tag="ACHKA1_002164"
     CDS             40653..40952
                     /locus_tag="ACHKA1_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5679817.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            40949..42346
                     /locus_tag="ACHKA1_002165"
     CDS             40949..42346
                     /locus_tag="ACHKA1_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO5679818.1"
                     /translation="MKLAPNVKLLPKDKHTEAVIFAGDNAHSFAEHYIIAQAKKAGDP
                     VPPVYLGRYQLSELDNLQIVDKGRFRAKVIRAGNIDEVQLLTIATKLAIAGVQEARLF
                     SENFELLEEWSDQLPRLRETWERGESLSHQRKTTLPMSVGSAGYDTQLDYVVKGIIPA
                     SSLCSIYGASGSYKSFLAGSWACHVSTGRHWGGRRVAHGAVLYVVGEGGIGVPRRVRA
                     WEVVHGEQVKNLYLVNRPIFPAAPFDVDEMVIAARQVERETGKPVRMIILDTLARCFG
                     GNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYRI
                     RREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDKPRPPVE
                     LERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKRLGYDVKNMRRWLAKLE
                     KDAVIYIDGDDVGPL"
     gene            42550..42801
                     /locus_tag="ACHKA1_002166"
     CDS             42550..42801
                     /locus_tag="ACHKA1_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001453954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679819.1"
                     /translation="MTKMRRDRTEPKYKALDMTEHALKVAIRVIDRHAGEGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            42798..43208
                     /locus_tag="ACHKA1_002167"
     CDS             42798..43208
                     /locus_tag="ACHKA1_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5679820.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            43219..43491
                     /locus_tag="ACHKA1_002168"
     CDS             43219..43491
                     /locus_tag="ACHKA1_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5679821.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            43539..>43608
                     /locus_tag="ACHKA1_002169"
     CDS             43539..>43608
                     /locus_tag="ACHKA1_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707048.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5679822.1"
                     /translation="MAHYRVFVTVRHSYYLKQTDTTE"
BASE COUNT        10896 a        11209 c        10912 g        10591 t
ORIGIN      
        1 gtcgtaacct tatgcccggt taaaggagag ggttatgacg aatatcacct tacagaaaca
       61 acatcgtaca ctgtggcatt ttattccggg gttagccctg agtgcagtta tcaccggggt
      121 cgccctgtgg ggtggttcca ttcccgcggt tgcgggtgcc gggtttagtg ccctcaccct
      181 cgcaatcttg ttggggatgg ttttaggcaa caccatctat ccgcacatct ggaaaagctg
      241 tgacggtggt gtgctgtttg ccaagcaata tttattacgt ctgggtatca ttctttatgg
      301 cttccgtctg acgttctcgc aaattgccga tgtcggtatc agtgggatca tcattgacgt
      361 gttgacgctg tccagtacct tcctgctggc ttgcttcctg gggcagaaag tgtttggtct
      421 ggataagcac accagctggt tgatcggtgc cggtagcagt atctgtggtg ctgccgcggt
      481 actggcgact gagccggtag tgaaagcgga agccagtaaa gtaaccgtgg ctgttgcaac
      541 cgttgttatc ttcgggaccg tcgcgatttt cctctacccg gcgatatatc cgctgatgtc
      601 tcaatggttt agtccggaaa ctttcggtat ctacattggt tctactgtgc acgaagtggc
      661 gcaggtggtg gcggcaggtc atgccatcag cccggatgcg gaaaacgcag cagttatttc
      721 caaaatgctg cgcgtgatga tgctggctcc gttcctcatc ctgctggcgg cgcgtgttaa
      781 acagctgtct ggggcgaaca gcggcgagaa aagcaaaatc actattccgt ggtttgccat
      841 cttgttcatc gtagttgcca tctttaactc gttccacctg ttaccgcaga gcgtggtgaa
      901 catgctggta acgctggata ccttcctgct ggcaatggcg atggcggcgc tgggtctgac
      961 cactcacgtc agcgcgctga aaaaagccgg ggcgaaacct ctgctgatgg cactggtact
     1021 gtttgcctgg ctgattgttg gtggtggtgc gattaactat gtgattcaaa gcgtcatcgc
     1081 ataaaccact acatcttgct cctgttaacc cgctatcatt accgttttcc tccagcgggt
     1141 ttaacaggag tcctcgcatg aaatacattg gagcgcacgt tagtgctgct ggcggtctgg
     1201 caaatgccgc aattcgcgcc gccgaaatcg acgcaaccgc gtttgccttg ttcaccaaaa
     1261 accaacgtca gtggcgtgcc gcaccgctca cgacgcaaac catcgatgaa ttcaaagccg
     1321 cctgtgaaaa atatcactac acatcggcgc aaattcttcc ccacgacagc tatctgatta
     1381 acctcggaca tccggtcact gaagctctgg aaaaatcgcg cgatgccttt atagatgaaa
     1441 tgcagcgttg cgaacagctg gggctttctt tgctcaactt ccaccctggc agccatctga
     1501 tgcagatttc agaagaggat tgccttgcgc gtattgccga atccatcaac attgcgctgg
     1561 ataaaactca aggtgtgacc gcggtgattg aaaacaccgc cggtcagggc agtaacttag
     1621 ggtttaaatt cgaacatctc gcggcgatta tcgacggcgt ggaagataaa tcccgtgttg
     1681 gcgtctgcat tgatacctgc catgctttcg ctgccgggta tgatttgcgt actccagccg
     1741 aatgcgagaa aacgttcgcg gattttgccc gtattgtcgg ctttaagtat ctgcgcggga
     1801 tgcaccttaa cgatgcgaaa agcacctttg gcagccgcgt tgaccgccat catagcctcg
     1861 gtgaaggcaa tatcggtcat gatgcgttcc gctggatcat gcaggacgac cgcttcgacg
     1921 gcattccgct gatccttgaa accatcaacc cggatatctg ggcagaagag atcgcctggc
     1981 tgaaagcgca acaaactgaa aaagcggtag cctgaagatg aataaccggg aaaaggagat
     2041 ccttgcaatt ttacggcgta acccgctgat tcagcagaac gaaattgcgg acatgctgca
     2101 aatcagtcgt tcgcgcgttg cggcgcatat tatggattta atgcgcaaag gccggattaa
     2161 aggcaaaggt tacattctca ccgagcagga atactgcgta gtggtgggga caatcaatat
     2221 ggatattcgc gggatggcgg atatccgtta cccgcaatcg gcttctcatc ccggtacaat
     2281 tcattgctca gcgggcggcg tgggacgcaa catcgcccac aatctggcgc tgttaggccg
     2341 cgacgtccat ttgctttcag tgattggcga tgacttttat ggcgaaatgc tcctggaaga
     2401 aacgcgccgt gccggcgtga atgtctccgg ctgcgttcgt ttgcatggtc aaagcacatc
     2461 gacgtatctg gcaattgcca atcgagacga tcaaaccgtg ctggcgatta acgataccca
     2521 tctgctggaa cagttgacac cgcaactact gaacgggtcg cgcgatttac ttcgtcatgc
     2581 gggcgtggta ctggctgatt gcaacctgac agccgaggcg ctggaatggg tctttaccct
     2641 cgctgatgag atcccggtgt ttgtcgatac cgtttcagaa ttcaaagcgg gcaaaatcaa
     2701 acactggctg gcgcatattc acaccctgaa acccacttta ccggagctgg aaattttatg
     2761 gggacaggcg atcaccagcg atgctgaccg taatgctgca gtgaatgcgt tgcatcagca
     2821 aggtgttcag caactgtttg tttatttgcc cgatgagtca gtttattgca gcgaaaagga
     2881 tggagaacaa tttttgctga ccgcaccagc gcatacgaca gtagacagtt ttggtgctga
     2941 cgatggtttt atggcgggcc tggtatatag ctttctggag ggaaacaatt tccgtgacag
     3001 cgcccgtttt gcgatggcct gcgcggcaat ttcacgcgcc agcggcagct taaacaaccc
     3061 taccctgtct gccgataacg cgctttcatt agtgccaatg gtgtaacaat gttgccggat
     3121 tcgacgcttg accgcatctt atccggctca taaatcgcac actacgctaa accaataaag
     3181 aacccggcaa tggtggcact catcaggtta gaaagtgtcg ccgccgccag cgcgcgtaaa
     3241 ccaagctggg cgatttccgg cgcacggtgt ggcgcaaccg cagaaaacgc ccccaccacc
     3301 accccgatag aaccaaagtt agcgaaaccg cacaacgcga aggaaataat cgccacggtt
     3361 tttgcatcga gagtgccagc tgtttgcaga tagggtgaga aattgagata agcgacaaat
     3421 tcatttattg ccagtttctg tccaatcaaa ctcccggcaa gattcgcatc agtccagtca
     3481 acacccatta cccacgccag tggggccagc aggtaaccta aaatggactc cagcgaggca
     3541 tgttcaaaac caaaccagcc gccaacgccg ccgataatac cgttaattaa cgcaatgatg
     3601 gcaacaaatg ccatcaccac tgttgccaca cccgcagcga ttttcagccc ggtcaccgcc
     3661 cctgtcgcgg cggcttcaat aatgcttttt ggcggtgttt cggtgaaaga gaggttatta
     3721 aaggaaacct gtgaagattc cgtagccggg cttagcatgc gggcaaacaa gatcccgccg
     3781 gggatcgcca ttaacgatgc cgccagcaaa tattccacag gcacgcccag tgcggcgtaa
     3841 ccaatcatcg tcgaaccagc aattgaggcc atgccactac aaatcgctgt aaataactca
     3901 ttgcgattca gccgatcgat aaagggtttg acgattgccg gaatttcgtt ttgcccgagg
     3961 aaaatggtag tgaccgcgac gaatgactcg atcttgctga tatttaatgc tttctggaat
     4021 ataccgccga gaatgcgaat taagatcccc atcacaccga tgtagtagag aatactcacc
     4081 agcgcggtga cgaagataat tgccggtaac accctgaaac caaagataaa tcctgcgcca
     4141 tcaaataagg tgtccatttt cggtccgacc agagaaccga agataaatgc gctacccgcg
     4201 tcgctgtacg ccatcacttt atgcacgcca aaagcgactt tttcagcgac ccaacgccct
     4261 ggcggtaacc aaagcataat gccgccaatc acgacctgta acactaacgc cgcgccaacg
     4321 gtacgcaggc tgatcttctt cttgtttact gacagtaaaa acgcaatcgt cagcaatacc
     4381 accattccca gaacacttct catgacatcc atagttatta tctcttcatg tcatattggg
     4441 cgacggcttt ctgcccctca gaaatagccg ggcctgcatc ttgcgatact gacccggctt
     4501 gttatcaaca cttaatgggt tttgatgtag ccgcgcacgc actcttcgac taagccccag
     4561 aaccagtcgg tatcaatagt gatgccgact ttggtattag cgggcttgcc aagaacgccc
     4621 agctcgtcgc agacggtacg cccatagcaa gggccactgt tgacgtccac ttcgacgtac
     4681 atctcctggg ttttaatgcc atcagggtta atcagataac cgatgcaggt ggcgtcgtgc
     4741 accgggccgc cagccaggcc gtagttttcg aactgggttt tgagagtgaa gttcatgatg
     4801 tcgctgaaca gctctccggc agggccgcct gccctttcca tccgagcaat tacgtccggg
     4861 gtgcaaacgg tctggttggt aagatcgagg cccatcatca ctaatggaac gccggaggtg
     4921 aacaccacgc gtgcggcttc cgggtcagca aagatgttga actcggcaga tggcgtgaag
     4981 ttgcctgtac cgtaagcgcc gcccattagc acaatttcac ggattttggg caggattgcg
     5041 ggttgcatac gcattgccac cgcgatattt gacaacggac caaccggcac cagagtgata
     5101 tcgccatcgc ttgccatcag ggtatcaatg atgtatttca ccgcgtgggt actttctgcc
     5161 tggcgggtca gcggctcgaa taccgggcca tccagtccgg tttcaccgtg aatattatcg
     5221 gcaacgattt gttgacgcat aatgggttgc ggcatccccg cataaaccgg aacattaatc
     5281 tccagtttct ggcaaacatt caggccatta attaatgttt tatcaagcgt ctgattaccc
     5341 gcaacaatgg tgatacctaa taaatcaatc gccggatgtt tcgccgccat cattatagca
     5401 atagcatcat catgacccgg atcacaatcc agaataattt ttctcttttc cattgtttat
     5461 ttcctctgtt tccagttgcg ttattttttc tacagcaaag aaatttcgat cgggccgatg
     5521 attgaatctt aacaacagcg tacgtatgct aaatatgaga aatctcatag cggataaaca
     5581 tcgtgaaaga aatccacaat aatgatctta agcagcaatt gatgagtgaa tccgcgttta
     5641 aggattgctt ttcaacggat gtttcagccg atacgcggct gtttcatttt ttagcgcgtg
     5701 actacattgt gcaggaaggg caacagccgt cctggctgtt ttacctgacg cgaggccgcg
     5761 ccaggcttta cgccacgcta gctaatggtc gcgtgtcgct gatcgatttc tttgccgccc
     5821 cctgttttat tggtgagatt gagttaatcg ataaagacca tgaaccgcgt gcggtacagg
     5881 ctattgaaga gtgttggtgc cttgcgctcc ctatgaaaca ttaccgtccg ctgttattaa
     5941 acgacaccct atttttacga aaactctgcg ttactttaag ccataaaaat tatcgcaata
     6001 ttgtttcttt aactcagaat caatcatttc cgttagtgaa tcgcctggca gcatttatat
     6061 tactctcaca agaaggtgat ctttatcacg aaaagcatac gcaagcggca gagtatttag
     6121 gcgtttctta tcgacatctt ttatatgttc tcgcgcaatt cattcacgac ggtttattaa
     6181 ttaaaagcaa gaaaggatat ctaattaaaa acagaaagca gttgtcaggg ctggcgctgg
     6241 agatggaccc ggagaataaa ttctccggga tgatgcagta aaaattattt gcaatagcgc
     6301 gattgccgga tgcaacgctt aataagcgag tacatccggc aatgaaactc aagctaaacc
     6361 aataaagaac ccggcaatgg tcgcgctcat caggttggaa agcgttgctg ctgccagcgc
     6421 ccgtaaacca agctgggcga tttccggagc acgttttggc gaaatagccg aaaatgcccc
     6481 aacgacaaca ccgatagaac caaagttagc aaaaccacaa agcgcaaagg agataatcgc
     6541 aatggttttc acttccagcg tgccgcccgt ttgcaggtag ggggagaaac tcaggtaagc
     6601 gacgaattcg ttaatcgcca gtttctgccc aatcaggcta cccgcaaggt tggcatcact
     6661 ccagtccaca cccatgatcc acgccagcgg tgccagcaca tagccaaaaa tactttccag
     6721 agaagcattg gtaaaaccaa accagccgcc aattccgccg ataatgccgt tgatcagcgc
     6781 aataattgcg acaaacgcca ttaccaccgt cgccacaccg gcggcgattt ttagcccggt
     6841 catcgcaccg ctcgccgccg cttcgataaa gctttttggc ggcgtttcac tgaacgacag
     6901 attttcaaat gtgacctgcg aaggctcggt tgccggacta agaatacgtg caaacaaaat
     6961 accgccgggg atcgccatca gcgatgccgc caacaggtag tcaattggta cgcccattcc
     7021 ggcataacca atcatcatcg aacccgcaat ggacgccatc ccgctacaaa tagccgtaaa
     7081 caactcgttt cgattcatgc gatcgataaa cggtttaacg atcgccggga tctcattttg
     7141 cccgaggaaa atagtggtga ctgcgacaaa agattcgatt ttgctgatgt tgagggcttt
     7201 ctggaaaatg ctgccaagga tgcgaattag cagccccatc acgccaatgt agtacagcag
     7261 actaatgagc gcagtgacga agataatcgc cgggagtacg cgaaaggcga agataaaacc
     7321 cgcaccgtca aagaggacat ccattttcgg cccgaccagc gaaccaaaaa tgaaggcgct
     7381 accggcatca ctgtaagaca tcactttatg aacgcctaat gccgcctgtt ctactgccca
     7441 ttttcccggc ggaaaataga gcataatgcc accaatagcg atttgcagca gcagtgcggc
     7501 tccaaccgtg cgcaaactga tgctcttttt atttaccgac aacagaaatg ctattgccag
     7561 taacaccacc atccccacaa cacttctcat tatatccata atgattttcc cttcatgcca
     7621 gtaaacccgg cgtcagcgcc aggttttggt atgtttgatg agtacgggcg acggctttct
     7681 gcccgtcaga aattaacccg cgagacgctg atattctttg gcaatttcgc tcgccagaat
     7741 ggcgttgttg aacaccagct ggatgttgga tttcaggctg tcaccgccgg tcagctcagc
     7801 aacgcgcgcc agcaggaatg gcgtactttc tttaccaata acaccctgcg cttcagcttc
     7861 agctaccgcc tgatcgatcg ccgcattgat agtgtgttcc ggcatagcaa actgttccgg
     7921 gatcgggttc gctaccacga ggccaccgtt cagcccgctt tgccatttca ccgccattgc
     7981 acgggcaatt tcgctggcgc tgtcgagacg aatgctgacg tcaaacgggc tggtgcggca
     8041 gaaaaacgca ggcagcgctt ttgtctgata gccaattaac ggcacaccga aggtttctaa
     8101 atactcagtg gttaatccta aatcgagaat agatttcgcc ccggcacaaa caacggtgac
     8161 attagtattt gccagttctt gcaaatcggc agaaatatcg aaggtatgtt ccgccccgcg
     8221 atgcacacca ccaattcccc cggtggcaaa tactttaatt ccggcaagcg ccgcaataat
     8281 catcgttgaa gccacagtgg ttgcgccatt ttttccggcg gcaacaacaa aaggtaaatc
     8341 gcgacgacta actttggtca cgttatgccc ttcacgaccc agtaattcaa tttcttcttt
     8401 gcttaaaccc actttcatca cgccgccaat aatggcgata gtggcaggta cagcgccctg
     8461 tttacgaata gtttcttcaa cttcaattgc ggtctgggca ttttgtggga acggcatccc
     8521 gtgagaaata atggtcgatt ccagcgccac aaccggtttt ttgtttttta aagcgtcctg
     8581 cacttccggg gaaatttgta ataattcagg ggaaattttt aattcagaca ttctgcgttc
     8641 tccactaacg atataacgtt ggcaatcgat aaatcggggt tattggtgta ttcacaggag
     8701 agcgccattg acgaacatcc ctgtgcgaaa cgaacagatt cggcaaacgg cattccgtct
     8761 acccaacacg aagcaagtcc cgccatcatg gcatcgcccg ctccggtaac attaataaca
     8821 ttggttttga tcggcgcaga ccagccactt tcaccgctga tatcgctgta ataaacgcca
     8881 tcgccgccca tgctcaatac cagtcggttc aggccatgtt gatggaacca ggcagcaact
     8941 tttgccacat cttcacgccc tgacagcgca atcccactca gggtttccgc ttcaaggcgg
     9001 tttggcttga gagtgtggat cagatttaga cggtcgcgca ctttgacaca tttccacgcg
     9061 gaaacaggat cgacaaatac gggtacgttg gcggcattat ccagaatcca tgccagtgcc
     9121 tcttcactga tattacagtc cgcgacaatg acctttgcac tctgaataaa ttcacggtgc
     9181 tgtgcaagat attcagctgt aatagcgttg ctaatattca tgtcatttat agcaaccagc
     9241 atttcaccgg tattatcgag taatgataaa taactcgacg tattttctcc cggcacaatc
     9301 aggcatttat cgacataaac gccagattga ttggtttgcg ttagcagcga ttgaccataa
     9361 aaatcactgc ctacggcgct cagtagccag gctttgttac ccagcaacgc caggttttgt
     9421 gcaatattgc gccctactcc accaggcgta aattttattt tacctggatt tgaatccgca
     9481 taatttaatg attcatgtga atatccggcg acatcaatat tcgccgagcc tataattacg
     9541 acataatcct tttcgcgcat agcacgtcct ctggtaaaaa attaccaacc ctgtcaatcc
     9601 acaagaatat agatactgca aagacggttt aaacatgtgt tcatatttaa acatgtgctc
     9661 atagtatgca tgttttacgt aaagtaaatt accgccagag aggcaaaatg ggcagatttg
     9721 agcaggctca caaaatgcga ttaaatgcct gaattacaaa agcgaggaat ttgtgacgag
     9781 atgcacagaa caggaacact aaaaaagggc agaaaagaat ctgcccttga ggaaataacc
     9841 ttagtatttg atgaacgttt caggtttcta ctgaaagata aaaccattta aattcataaa
     9901 attacattca caaaccggtc attctctttg cgctcatcca atgatacagg gcagtaaatt
     9961 tctgccctgt aacacacctt ttattacgcg gctttcgcta ctgcgtccac ttccggacgt
    10021 ttcaggaagg cataggccaa actcgccacc agcgtaccgg caataattgc taccaggtaa
    10081 cccagtaccg gcgtaatagc gccagggatc agcagaacaa acagaccacc gtgcggtgcc
    10141 atcagtttcg caccaatcgc cattgagatt gcgccagtca gcgccccacc aacgatacaa
    10201 cacggcagca cacgcatcgg atcacgagca gcaaacggaa ttgcaccttc cgaaataaag
    10261 cacagaccaa gaaccagagc ggctttgcca ccttcctgct gcgctttgtc gaatttgcga
    10321 cgcgccacca ttgttgccag acccattgcc agcggtggca ccatacctgc cgccataatc
    10381 gccgccatcg ggccataggt ttgagtactc agcagaccca caccgaatgc gtacgctgct
    10441 ttatttaccg gaccgcccat gtcagtacac atcatgccac cgaggatcgc ccccagcaga
    10501 accgcattcg cagtccccat ggtctgcagc cagtgagtca acccttcgag aatgccagca
    10561 accggtttac cgatcaggta gatcatcgcc agaccgacca ccagactgga aattagcgga
    10621 atgatcagga tcggtttcag cgcctccata ctctgtggca gtttcagttg cgtactgatt
    10681 aacttcgcaa tgtaaccagc caggaagccc gcaataatac cgccaatgaa gccagaaccg
    10741 gtgctgaccg ccagcatacc gccaatcaga cccggcgtga gacccggacg atcggcaatg
    10801 gagaaggcaa tgtaacctgc cagtaccggc accatcagcg caaaggctga accaccacca
    10861 atctgcatca gcgccgctgc caacgtaccc ggctctttaa acgcttcgat accaaaagca
    10921 aaagaaagcg cgatacacag accacctgca acgaccatcg gcagcatgta agagacgccc
    10981 gtcagcaagt gacggtatgc gcctgcactc tctttcttac cttcagaggt cgccgtttga
    11041 gctttgcccg ccggttcata cggcgttgct tcagcaaccg ctttatccag ttcctgcgcg
    11101 gttttcttca gcgccagacc ggtagaggta cgatacatcg gtttaccagc aaatttcgcc
    11161 agatccactt cgatatctgc cgccacaatc accagatccg ctgcggctac ttcttcggga
    11221 gtgattgcat tacccgcacc aacagaacca cgggtttcaa ctttcaccca ccagccacgt
    11281 tttttcgctt cggtttcaat ggcttcagcc gccataaagg tgtgtgctac gccagtcggg
    11341 caagcagtca ccgcaactac gcgtttcgga ccgctggcgg caaccggtgc tgtcgcagcg
    11401 accggcgcag tgtaaggttt cgcatggcct ttggcttcac tcaggaacag ctcagggtgc
    11461 gcaactgccc gggaaatatc gcccagccag acatttttac cgttcagcgc gctgtcattc
    11521 gggatggaat caccgagaac aatcgccatt tctgcgtcat tcggattgtc gatgatttcc
    11581 agttttgctt ttcgcgccgc cgcgcccagc agggtcttcg ccatataggc gcgtgcctga
    11641 ccgagattag cgtcaataat cagcagcgtt ttcattatgc ctctcctgct gtcagttaaa
    11701 aggttgtaag tcgacgcgcg ccatcattgc ggccaactgc ggacgatcgg taatacccac
    11761 attgctttga cttaccgcca gggctgcaac agcagtcgcc agacgcagtg tgtgttcact
    11821 ggattcacgc attagcaagc cataaatcag gccaccaacc atagaatccc ctgcgccaac
    11881 ggtgcttacg acatcgactg acggtggttt ggcgatccat tcgccggagg cattaaccca
    11941 aagcgcgcct tcggcaccca gtgaaataac gacatgcgca ataccttgtt cacgcagcgc
    12001 atgcgcagct tcaatcacat ctttcatttc aggcagttta cggcctgccc agatttccag
    12061 ctcgcggcgg ttaggtttca ccagccacgg tgccgctttc aaacctgcta ctaacgcttc
    12121 acggctacta tcaaagataa tgcaagggca ctgactacgc aggcgagtca tccagtcggt
    12181 gaacgcttcc gggctgacgc ctgacggtaa gcttccgctg acacagacca tatcgaactg
    12241 accgagccag ctcagagaat cagtcacaaa gcgttcccag tcggcgggag tgacttcaaa
    12301 acccgagaag ttgaagtcgg tcacttcgcc gtctttttcc gtcagcttaa cgttaattcg
    12361 ggtgcgcccc tgtacaacct ggaaacggtt ggcaatgccc agctcgctga acagttgctg
    12421 aaaaccatcc tgattgtctt taccgaggaa gccgccaacg gtgacatcaa ttcccagatc
    12481 ttttaatact ttggccacgt tgatgccttt acccgccgca tgcagaccgg tggttttcac
    12541 caggttcact tcgccgcgtt caatttccgg gcagaaacca acaaggtcat aagccggatt
    12601 aagggtgata gtagcaacac gtctgctcat tatgcgccct ccccaagacc agcagcgata
    12661 gcgtcgccga ttgctttcag cgcctgttca gcatctgcac cctgggcggt aaagcgtagg
    12721 cgatgacctt tcttaacgcc aagtgccaca actttcatca gactacgtcc gtttgccggt
    12781 ttgccggtgc catcaaggtt tgtcacggta atatcactgt taaattgttt aatggtgttg
    12841 accagcatgg tacctggacg agcatgcagg ccgtgttcat tgcgcaccac aaactccgcg
    12901 cttaacacgt cgtcggtcgg cgcatcatcg ctggtcagca gcgccagcaa cgttgccgca
    12961 tccgctttca gcaagcggtc agctttattg tcgagcaaca aatcagcgag acgcttaaga
    13021 accgcaatgg gctgatcgtc attcatcgcc acactcacca gcatggctgc cgtttcgccg
    13081 tccacatcaa aagcatttgc cgcacggctt accgcaatcg cgctacgcaa attgccttcg
    13141 gcgctatcgc tcagccagat gccctgtccg aggttcagcg gttgttcatt gatggctttg
    13201 gtgacgaaag tggcgtcaac tgcccccgct tctttcagac gcgcagcgtt cagcgcctga
    13261 agagtcagca gatcgctggc gacgatatcc agcgtcagca tttcgttgtc gagcttcagc
    13321 tgctcactct gcttttcgcc catcagcaat gcgcgaagtt cttctgctgt tgttgctgac
    13381 ttcagttgtt cagcaacgga atcatcgctc aggacgtggg tcagctggcg tagcaggccc
    13441 agatgttcat ccgagctggc agcaataccg attgccacgt acgctacctg accgtcaccc
    13501 caggtgacgc cttccgggaa ctgaaatacc tgaacgccgg ttttcagcac ctgatcgcgg
    13561 gtgtcggtag tgccgtgtgg aatagcaata ccattgccga ggaacgttga ggtttgctgt
    13621 tcgcgcgcca gcatgccatt gacgtagcct tctgctacat taccggcctg caccagcgcc
    13681 gcagcgacct ggcgaatcgc ctcttctttg tctccggcct tttcgcccgg atggatgtcc
    13741 tgtacggata actggaacat agttctcctc tcttgctgaa ttgaaacgat tcagcctcta
    13801 tgagaaaaaa agcgccaacc tagcttaggg ttaaagacaa gatcgcgctg aaacgtttca
    13861 agaaagcata atacttctgt ttcagcacgc aaggaaagcc gtaaagttat gagcaaaagt
    13921 ttgatgtgct gcacattttt ttgtcatttt tcctaattgc tgatgggaaa atccgttatc
    13981 agcaatttca tttcagcagc gtcagcagcc aatcagcaac agtgaaatgg cattttgatt
    14041 attcgtggcg cttttgattt cgctgtctgt ttattttctg acaagcagcg taaactccgc
    14101 gtcttcctct tccagtgatc gaccagcatg cataactccc ccgcagtctc cagcgcgaaa
    14161 tcgtttgacc tgacctcgac ggcgttttta atcgttgcct ttctcaccgg tattgcgggc
    14221 gctctgcaaa ccccgacact cagtattttt cttaccgatg aagtacatgc ccgtccggcg
    14281 atggtgggat tcttctttac cggcagcgct gtcattggga ttctggtgag tcagtttctc
    14341 gccgggcgct ctgataagcg cggcgatcgc aaatcgctga ttgtcttttg ctgcctgtta
    14401 ggcgtgctgg cctgcaccct ttttgcctgg aatcgcaact actttgtttt gctattcgtt
    14461 ggcgtctttc ttagcagctt tggctcgacc gctaacccgc aaatgtttgc ccttgcccgt
    14521 gaacatgccg acaaaaccgg acgtgaggcg gtgatgttca gctctttttt acgcgctcag
    14581 gtttcactgg catgggtcat tggcccaccg ctggcttatg ccttagcgat gggtttcagc
    14641 tttacggtaa tgtatctgag cgcagcggta gcatttattg tttgcggcgt gatggtgtgg
    14701 ctgtttttac cgtcgatgca aaaagagctt ccgctggcga ccggcacggt tgaagcgccg
    14761 cgccgtaacc gtcgcgatac gctgctgctg tttgtcattt gtacattgat gtggggctcg
    14821 aacagtctgt acatcatcaa catgccgcta tttataatca acgaactgca tcttcccgag
    14881 aaactggcag gcgtaatgat ggggaccgcc gccgggctgg aaatcccgac catgttgatt
    14941 gccggatatt ttgccaaacg tctgggtaag cgtttcttaa tgcgcgttgc tgccgtgggt
    15001 ggcgtctgtt tttacgcagg aatgctgatg gcgcattcac ctgccattct gttgggcttg
    15061 cagctgctaa atgctatttt tattggcatt ctgggcggca tcgggatgct ctattttcag
    15121 gatttgatgc ccggtcaggc aggttcagcc accacgctct ataccaacac gtcgcgcgtg
    15181 ggctggatca tcgcaggatc tgtagcgggc atcgtcgccg agatctggaa ttatcacgct
    15241 gtgttctggt ttgcgatggt gatgattatc gccactctgt tttgcttact gcggattaaa
    15301 gatgtttaag gcgcggtcag catttccaga tcgataagcc aggtcattgc ctgctgacgc
    15361 gagttaccgc acatttcagc gctggcttgt aagcctgcac agactttcgg ccgcagtggc
    15421 gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa tgcagggcgt attggcgggc
    15481 ttgccatctg gcataccggg aatagggctg gaaattgaag gggcagtgca acacgccccg
    15541 caacccggac ggcattccat acgttgttct cttaaggtta accaccggat tgatgagcgc
    15601 gcacagtacc accttttttg caccagcaaa agtgcgaata ccacttgccc gaaaggcccg
    15661 tcgcgagtac tttgtcgcga tatttttgac attttcgact acaggaattt ttcgatgcca
    15721 agagcgaacg aaattaaaaa aggtatggta ctgaattaca acggcaaact gctgctggtt
    15781 aaggacattg atattcagtc gcccactgcc cgcggtgccg ctacgctgta caaaatgcgt
    15841 ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt tcaaaggaga tgatatcgtt
    15901 gacaccgtga cgctgacccg ccgttacgtt gacttctcct atgtcgatgg caacgaatat
    15961 gtctttatgg ataaagaaga ctataccccg tataccttca ccaaagatca gattgaagaa
    16021 gagttgctgt ttatgccaga gggcggcatg ccggacatgc aggtgctgac ctgggacggt
    16081 caactgctgg cgcttgagct gccgcagacc gttgatctgg aaatcgttga aaccgcacca
    16141 ggcatcaaag gtgcatctgc cagcgcccgt aacaaaccgg cgacattgag cactggtctg
    16201 gtgattcagg taccagaata cttaagcccg ggcgaaaaaa ttcgtatcca tatcgaagaa
    16261 cgccgttata tggggcgtgc tgactaactt cagccgcatg cagaaaaggg atagctcagg
    16321 ctgtcccttt tttaatttat tataccaatc ttctattttg cgcttcctga acaccttacg
    16381 cccgcaattt tcactcgttg attgatcgcc ctcacacttc atcgcattaa caatccagac
    16441 caatttcaat tgctgtcata taactttaca ctgtcattgt taattaatcg ttactaagac
    16501 gtgactccta tgaatacaat cgcctccgtt acgctcccgc atcatgtaca cgctccacgc
    16561 tacgatcgcc agcagttgca atcacgtatc gttcattttg gctttggagc ctttcaccgc
    16621 gcacatcagg cgttactgac cgatcgtgtg ctgaatgccc agagcggcga ctgggggatc
    16681 tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc agctccgcgc acagaaccat
    16741 ttatacaccg tgctggagaa aggggcggac ggcaatcagg cgataattgt cggtgccgtt
    16801 cacgaatgcc tgaatgcaaa actggattcc ttagcggcaa ttattgagaa attttgcgag
    16861 ccacaggtgg caattgtttc cctgacgatt accgaaaaag gctattgtat tgacccggcc
    16921 accggtgcac tcgacaccag caatccgcgg attattcacg atctacaaac ccctgaagaa
    16981 cctcactccg caccgggtat tctcgtcgaa gcactgaaac gccgccgtga gcgcggcctt
    17041 acaccgttta ccgtgctctc ctgcgacaat attcccgaca atggccatgt ggtgaaaaac
    17101 gcggtgctgg gaatggcaga aaaacgttcg ccagaactcg ccgggtggat aaaagaacac
    17161 gtcagttttc cgggaaccat ggtcgaccgc attgttccgg ctgcaaccga cgaatcactg
    17221 gcagaaatca gccagcatct gggggtaaat gatccctgcg cgattagctg cgaaccgttt
    17281 atccagtggg tggtggaaga taacttcgtc gctgggcgtc ctgcctggga agtcgcaggt
    17341 gtacaaatgg tgaatgatgt cctgccatgg gaagagatga aactgcgtat gcttaatggc
    17401 agccactctt ttctcgctta tctgggttac ctctcaggat tcgcccatat cagtgattgc
    17461 atgcaggatc gcgcatttcg ccatgctgcc agaacattaa tgctggatga gcaagcgccg
    17521 acactgcgga ttaaagatgt cgatttaaca caatatgcgg ataagttaat tgcacgtttt
    17581 gctaatccgg cgctgaaaca taagacctgg caaatcgcga tggatggcag ccagaaatta
    17641 ccgcaacgca tgctggcagg tattcgcata catctggggc gcgaaacgga ctggtcgttg
    17701 ctggcattag gcgttgcagg ctggatgcgt tacgtcagcg gcgttgatga tgccggaaat
    17761 gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg atcttgttgc gggcagcagc
    17821 agtgagcaac gcgtcaccgc cctgctttcc ctgcgtgaag ttttcggtga tgatctgcca
    17881 gataacccgc attttgtgca ggccatcgaa caagcctggc aacaaatcgc acaattcggc
    17941 gcgcatcagg cgctattaaa caccctcaaa atttaacgat ttctgcggtt aaagcggatg
    18001 aagctcgcct tcgtccgctc tccccttctc ttttctgccg tttttagcca ggattaacgc
    18061 tcagttaact taccagagta ttgcggagcc gttgtgacca ggaccaacct catcaccggt
    18121 tttctcggca gcgggaaaac cacgtcgatt cttcatctgt tagcccataa agatcccaac
    18181 gaaaaatggg cggtactggt taatgaattt ggggaagtcg gaattgatgg tgctttgctc
    18241 gccgatagcg gcgcactgct gaaagagatc cccggcggct gcatgtgctg cgttaatggt
    18301 ttacccatgc aggtagggtt gaatacctta ctgcgtcagg gaaaaccaga ccgcttgttg
    18361 atagagccga ccgggctggg ccatccgaaa cagatcctcg atctcttaac cgcaccagtc
    18421 tatgaaccgt ggatagatct acgcgccacg ttgtgcattc tcgatccacg cctgctgctg
    18481 gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc tggctgccgc agacatcatt
    18541 gtcgccaata aatccgaccg tgcgacacct gaaagcgagc aagcgctaca gcgctggtgg
    18601 cagcaaaatg gtggcgatcg gcaattaatt cacagtgagc atgggaaagt tgacggtcat
    18661 cttctggatt tgccgcgtcg caatttagcc gagttgcccg ccagcgccgc gcattctcat
    18721 cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc cagagcatca acgctggcgt
    18781 cgcagtctga acagtggaca aggacatcag gcctgcggct ggatattcga cgctgatacg
    18841 gtattcgaca ccattggcat tctggaatgg gcgcgacttg caccggtgga acgcgtcaaa
    18901 ggcgtgctgc gtattcccga agggctggtg cgaatcaacc gtcagggcga tgacctgcac
    18961 attgaaacgc aaaacgttgc gccaccggac agccgtattg agctgatttc cagcagcgaa
    19021 gctgactgga atggcttgca gagcgcgctg ttgaagcttc gtttagcgac taccgcgtaa
    19081 ggttgcctgc gtttttcagt aagataatta gagaaaatat gattaaaaat ttgccgcaaa
    19141 tagtgttgtt gaatattgtc ggcctagcgc tgtttctttc ctggtatatt cccgttaatc
    19201 atggattctg gttgccgatt gatgcggata ttttttattt ctttaatcag aaactggtcg
    19261 aaagtaaggc ctttttgtgg ctggttgcat tgaccaacaa tcgcgctttc gacggttgtt
    19321 cactgctggc gatgggaatg ttgatgctga gtttctggct gaaagaaaac gccccaggca
    19381 gacgacgtat cgtgattatt ggtctggtca tgctattaac tgcagtggta ttaaatcagc
    19441 tgggtcaggc attaattcct gtaaaacggg ccagcccaac attgactttt accgatatta
    19501 accgcgtcag cgaactgctc tctgtcccca cgaaagatgc ctcacgagat agctttcccg
    19561 gcgatcacgg catgatgctg cttatttttt cggcattcat gtggcgttat ttcggcaaag
    19621 ttgcaggtct tatcgccctt attatttttg tggtttttgc gtttcccaga gtaatgattg
    19681 gcgcacactg gttttctgac atcattgtcg gttcgatgac cgtgatattg atcggtttgc
    19741 cctgggtgtt gctgacgcca ttaagtgatc gattaatcac cttttttgac aaatcactac
    19801 cgggaaaaaa caaacatttc caaaacaaat aactcacagt aattaacatc atcaggttta
    19861 tttttatagt gaggataatc ctgatgatgc gcaccgtgct ttcatccatc gaaagcaaaa
    19921 atcattctct aagtaaatga atggattgca tgcgtttcac tcaatcgtac tttaattgac
    19981 caatcccgct tattaacttt ctgtatcact ttttcttata aaaaatcatg taaaaccgct
    20041 cgccaagacc gcaccaatcg ggtaatctcg aactcgtttt gcctcggcgg tagattatcc
    20101 tcacagcata taattttgtg cgttagtcca cagatttggc cttaaggaat tgtttcaaca
    20161 tgcccaggta attagtctcg tgtcgcttgg cattttttta taacgatatt tgtcgttaag
    20221 gacttcaagg gaaaacaaac aacatggtca aatctcaacc gattttgaga tatatcttgc
    20281 gcgggatccc cgcgattgca gtagcggttc tgctttctgc atgtagtgca aataacaccg
    20341 caaagaatat gcatcctgag acacgtgcag tgggtagtga aacatcatca ctgcaagctt
    20401 ctcaggatga atttgaaaac ctggttcgta atgtcgacgt aaaatcgcga attatggatc
    20461 agtatgctga ctggaaaggc gtacgttatc gtctgggcgg cagcactaaa aaaggtatcg
    20521 attgttctgg tttcgtacag cgtacattcc gtgagcaatt tggcttagaa cttccgcgtt
    20581 cgacttacga acagcaggaa atgggtaaat ctgtttcccg cagtaatttg cgtacgggtg
    20641 atttagttct gttccgtgcc ggttcaacgg gacgccatgt cggtatttat atcggcaaca
    20701 atcagtttgt ccatgcttcc accagcagtg gtgttattat ttccagcatg aatgaaccgt
    20761 actggaagaa gcgttacaac gaagcacgcc gggttctcag ccgcagctaa taaaccgttt
    20821 ggatgcaatc ccttggctat cctgacgagt taactgaaag cactgcttag gcagtgcttt
    20881 tttgttttca ttcatcagag aaaatgatgt ttccgcgtct tgatccaggc tatagtccgg
    20941 tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat taacggaatc atgttcatac
    21001 gcgctcccaa ttctggacgt aagctcctgc ttacctgcat tgttgcaggc gtgatgattg
    21061 cgatactggt gagctgcctt cagtttttgg tggcctggca taagcacgaa gtcaaatacg
    21121 acacactgat taccgacgta caaaagtatc tcgataccta ttttgccgac ctgaaatcca
    21181 ctactgaccg gcttcagccg ctgaccttag atacctgcca gcaggctaac cccgaactga
    21241 ccgcccgcgc ggcgtttagc atgaatgtcc gtacgtttgt gctggtgaaa gataaaaaaa
    21301 cattctgttc atctgcgacc ggtgagatgg acattccact caatgaattg attccggcgc
    21361 tcgacattaa taaaaatgtc gatatggcga tcttacccgg cacgccgatg gtgccgaaca
    21421 aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa aaatagcggc gtctttgccg
    21481 ctctgaatct caacctgacg ccttcactct tttatagttc acggcaggaa gattacgatg
    21541 gcgtcgccct cattattggc aatactgcgc tatctacctt ttcttcacgt ttgatgaacg
    21601 ttaacgaatt aaccgacatg ccagtccgtg aaactaaaat tgcgggcatt cctctgaccg
    21661 ttcggcttta tgcagatgac tggacatgga acgatgtgtg gtacgcattt ttactgggcg
    21721 gcatgagtgg aactgtcgtt ggcctgctct gctattacct gatgagcgta cgtatgcgcc
    21781 ccggcagaga aatcatgacc gccatcaagc gcgaacaatt ttacgtggcg tatcaaccgg
    21841 tggtggatac acaagctttg cgagtaacgg gcctggaagt actgctacgc tggcggcatc
    21901 ctgtcgcggg agaaattccc ccggatgcct tcattaactt tgccgaatcg caaaagatga
    21961 ttgtgccgct gactcagcac ctgtttgagt taattgcccg cgatgccgca gaattagaaa
    22021 aagttctgcc ggtaggcgtc aaatttggca ttaacattgc gccggaccat ctgcacagcg
    22081 aaagctttaa agcagatatc cagaaactgc tcacttccct acccgcacac catttccaga
    22141 ttgtgctgga aattaccgag cgcgatatgc tgaaagagca agaagccaca caactcttcg
    22201 cctggctgca ctcggtcggc atagaaattg ctattgatga cttcggcacc gggcacagcg
    22261 cgcttatcta tcttgagcgt tttacgctcg attatctgaa aattgaccgt ggatttatca
    22321 acgccatcgg tacggaaacg atcacttccc ccgtacttga cgcggtgctg acgctggcga
    22381 aacgcctcaa tatgctgacg gttgctgaag gggtcgaaac gccggaacag gcgcgatggc
    22441 taagcgaacg cggcgttaat ttcatgcaag gctactggat tagtcgcccg ttaccgctgg
    22501 acgattttgt ccgctggctg aagaaaccgt atacgccgca gtggtaaggt gtgcttacgt
    22561 cccttattat tcatagtgaa agcatgccgg attgcggcta atgatgagta aaaggaaatc
    22621 cgttgcagat gattgtgcgc atactgctgc tgtttatcgc tctgttcacc tttggtgcgc
    22681 aggcgcaggc tatcaaggaa agctatgcct ttgccgtgct gggcgaaccc cggtacgcgt
    22741 ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc aaaaggtggg cagataacgt
    22801 tgtcagccct cggtaccttc gataatttca accgctatgc actgcgcggc aacccgggcg
    22861 cacgcaccga gcagctgtac gacacgctat ttacgacttc cgatgacgaa ccaggcagtt
    22921 attacccgct gattgctgaa agcgcacgct atgctgacga ttattcctgg gtggaggtcg
    22981 ctattaatcc gcgcgcccgt tttcatgatg gttctcccat tactgcccgc gatgtagagt
    23041 ttacttttca aaaatttatg accgaaggcg tgccgcaatt tcgtctggtc tacaaaggca
    23101 ccaccgtcaa agccattgcg ccgttaaccg tgcgcattga gttagctaaa cccggcaaag
    23161 aagatatgtt gagtctgttt tcgctgccgg tatttccaga aaagtactgg aaggatcaca
    23221 aacttagcga tccgctcgcc acgcctccgc ttgccagtgg tccgtaccga attacgtcct
    23281 ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga ttactgggca gcaaacttac
    23341 cggtaaaccg tggacgctgg aatttcgaca ccattcgcta cgattattac ctcgatgata
    23401 atgtcgcctt tgaagcgttt aaagcaggtg cttttgattt gcgtatggaa aacgacgcta
    23461 aaaactgggc cacgcgttat accggtaaaa atttcgataa aaaatacatc attaaagatg
    23521 agcaaaagaa cgaatcagcc caggatacgc gctggctggc gtttaatatc caacgtccgg
    23581 tattcagcga tcgccgggtc cgcgaagcga tcaccctcgc ctttgacttt gaatggatga
    23641 acaaggcgtt gttttacaat gcctggagtc gcacgaacag ttattttcag aataccgaat
    23701 acgcggccag aaattacccc gacgccgcgg agctggtgct tctggcacca atgaaaaaag
    23761 atctaccgcc agaagtcttc acacaaatct accagccgcc ggtatccaaa ggcgatggct
    23821 acgatcgtga caacctgtta aaagccgaca aacttctcaa cgaagcgggc tgggtgctga
    23881 agggtcagca acgcgttaat gccacaacgg gtcagccact cagctttgaa ttattgcttc
    23941 ctgcaagcag caatagtcag tgggtattgc cgttccagca cagcctgcaa cggctgggta
    24001 tcaacatgga cattcgcaag gtggataact cgcaaatcac caaccgcatg cgcagtcgcg
    24061 actatgacat gatgccgcgc gtatggcggg cgatgccgtg gcccagttcc gatttacaga
    24121 tttcctggtc atcggaatat atcaattcca cttataatgc ccccggcgtg caaagcccgg
    24181 ttatcgactc gctgatcaac caaattattg ccgcgcaggg aaataaagaa aaattactgc
    24241 cgttggggcg agcactggat cgcgtattaa cgtggaatta ttacatgctg ccaatgtggt
    24301 acatggcgga agaccgtctc gcctggtggg ataaattctc ccaacccgct gtacgccctg
    24361 tttacagcct gggtatcgat acctggtggt atgacgttaa taaagcggcc aaactgccgt
    24421 cagccaagca acagggagag tagatgggcg cttatctgat tcgccgtctg ttgctggtga
    24481 tcccaacgtt atgggcgatt atcactatca actttttcat cgtgcaaatt gcgcctggcg
    24541 gtccggtcga ccaggccatc gccgccattg agtttggtaa tgccggagta ttacccggcg
    24601 caggcggtga aggcgttcgc gccagccatg cgcaaacggg cgtcggtaat atcagcgaca
    24661 gtaattaccg tggcggacgc ggattagatc cagaagtgat cgccgagatc actcatcgct
    24721 acggtttcga taagccgctc cacgaacgtt acttcaaaat gctctgggac tacatccgct
    24781 ttgattttgg tgatagcctg tttcgcagcg cctcggtgct gacgctgatt aaagacagtc
    24841 tgccggtttc catcaccctc ggattatgga gcacgctgat tatctatctg gtgtcgattc
    24901 cgttaggcat tcgcaaagct gtttataatg ggagccgctt tgacgtctgg agtagcgcat
    24961 ttatcatcat cggctacgcc attccggcct ttttgtttgc catcctgctg attgtcttct
    25021 tcgcaggcgg cagctatttc gacctgttcc ctctacgcgg cctggtttcc gctaactttg
    25081 attcgctgcc gtggtatcag aaaatcaccg attatctgtg gcatatcacg ctgccggtgc
    25141 tggcgacggt gattggtggc tttgcggcgc tgactatgct gacaaaaaac tcattccttg
    25201 atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa aggggtaagt gaaaaaaata
    25261 ttctctggaa acatgtgttc cgcaacgcca tgctgctggt gattgccggt tttccggcga
    25321 cgtttatcag catgtttttt accggctcgc tgctgattga ggtgatgttt tcactcaatg
    25381 gtcttggctt actgggctac gaagcgaccg tctcgcgcga ttatcctgta atgtttggta
    25441 ccttgtatat tttcaccctg attggcctgc tgctgaatat tgtcagtgat atcagctata
    25501 cgctagtcga tccgcgtata gattttgagg gacgttaatg tcgcgactca gccccgtcaa
    25561 tcaggcccgt tgggcgcgtt ttcgccataa ccgtcgcggc tactggtcgt tatggatttt
    25621 cctcgttttg tttggtttga gtttgtgttc tgaacttata gccaacgata aaccgttgct
    25681 ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa aattacagtg aaagcgattt
    25741 tggcggcccg ctggcaagtc aggctgatta tcaggacccg tggctgaaac aacggctgga
    25801 aaataatggc tgggtactgt gggcaccgat tcgctttggt gctaccagta tcaactttgc
    25861 taccgataag cccttccctt ctcccccctc ccggcaaaac tggctgggaa cggatgccaa
    25921 cggcggcgat gtgctggcac gtattctcta tggcacgcgg atctcggttc tgtttggcct
    25981 gatgctgact ctctgttcca gcgtgatggg cgtgctggcg ggggcgctac aaggctatta
    26041 cggcggtaaa gtcgatctct gggggcaacg ctttattgaa gtatggtcgg ggatgccgac
    26101 gctgtttttg attattttac tttccagcgt cgtacagcct aacttctggt ggctgctggc
    26161 aattactgtc ttgtttggct ggatgagtct ggtcggcgtg gtgcgggcgg agtttttacg
    26221 tacccgtaat ttcgactaca ttcgtgcggc gcaggcgctt ggcgtcagcg atcgcagtat
    26281 catcctgcgt catatgttgc ctaatgccat ggtcgcgacc ctcacctttt taccgtttat
    26341 tttatgtagt tcgatcacca ccctgacctc actcgatttc ctcggcttcg gtctgccgct
    26401 cggttcaccg tcactcggtg aactgctgtt acaagggaaa aataaccttc aggctccgtg
    26461 gcttgggatc accgccttct tgtcggtggc gatattattg tctttgctga tctttattgg
    26521 tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg tagcatgacg caaactctgt
    26581 tagcgattga agatttgtcg gtgggttttc gccatcagca aaccgtacgt acagtagtca
    26641 atgatgtttc actacagatt gaggctggcg aaacgctggc gctggtgggt gagtcaggtt
    26701 caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct cccatccccg ccggttgaat
    26761 atctctccgg cgatattcgt tttcatggcg aatcgctgct tcatgccagc gatcaaacgt
    26821 tgcgcggtgt acgcggtaat aagatcgcca tgatttttca ggagccgatg gtgtcgttaa
    26881 atccattgca taccctggaa aaacagcttt atgaagtgct ttcactccac cgcgggatgc
    26941 gtcgggaagc ggctcgtggc gaaattctta actgccttga tcgcgttggt atccgccagg
    27001 cggcaaaacg gctgacagat tatccgcatc agctctccgg cggcgaacgc cagcgggtga
    27061 tgattgcgat ggcgctgtta acgcgaccgg aattattaat tgccgatgaa ccaaccaccg
    27121 cactggacgt ctctgtccag gcgcagattt tacagctgtt gcgcgaactg caaggcgagc
    27181 tgaatatggg catgctgttt attactcata acctcagcat tgtcagaaaa ctggcccacc
    27241 gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa taacgccgct acgctatttg
    27301 cctcacccac tcatccttac acacaaaagc tactcaacag tgaaccgtca ggcgatccag
    27361 tgccgttgcc agaacctgcc tcaacgttgc tggatgttga acagcttcag gttgccttcc
    27421 ccattcgcaa agggattttg aagcgcattg tggatcataa tgtggtggtg aaaaacatca
    27481 gttttacgct acgggcgggt gaaacgctgg gtttggtagg cgagtctggt tccgggaaaa
    27541 gcacgacggg actggcgctg ctgcgactga ttaattctca gggcagcatc atctttgacg
    27601 gtcagccact gcaaaattta aatcgccgcc agctgttacc tattcgtcat cgcattcagg
    27661 tggtatttca ggatccaaac tcctcgctca acccacgact caacgttttg cagattattg
    27721 aggaaggctt acgggttcac cagccgacgc tttctgccgc acaacgcgaa caacaagtga
    27781 tagccgtgat gcatgaagtg ggattagatc ctgaaacacg ccaccgttat ccggcggagt
    27841 tctctggtgg tcagcgacaa cgtattgcga ttgccagggc attaattctt aagccctcgc
    27901 tgatcatact tgatgaaccg acatcatcac tcgacaaaac ggtacaggcg caaatattga
    27961 cgctattgaa atcattgcaa caaaagcatc aactggccta tttgtttatc agccatgatt
    28021 tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact gcgacaaggg gaagtagtgg
    28081 aacaaggacc gtgcgcgcgc gtgtttgcca caccgcagca ggagtatacg cgtcagctac
    28141 tggcgttgag ctgacgctta aaaaggattg tagtttgaaa agggttcggc aattgccaca
    28201 ccaaaatttt tcaatcggca tgttgcagca aactcgtcct ggcgatttac aaacaggcac
    28261 ggctcacctt cacactccag caccgaacat ggaacttcga tgtcgcttaa cgcctggctt
    28321 agtttataca tcaccgacca tgcattatca ccatccgggc taagcaattt aagcgccacc
    28381 aggctgttgt caccgcacgg tccattttgc ggaatcggtt caaccttcga acctgcgaaa
    28441 ccagcagacc agcgatagtt ctgcggcagt cgatggacga ttgagagttg taatgaagtc
    28501 acttattttc cccggaagca catttacttc acaatttatt cacatcaatt ttaacacatc
    28561 atcaacaatc cgtctttata cagatcgtaa agattgatgc ctcgcctgcg tggcctctat
    28621 ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt gcggggctat atgtacccgt
    28681 ttctgcgatc taactcaacc tttttaacta caatgatgtg actttttaca taaattgatt
    28741 ttacataaaa taaacatata tcgggggaat gatagatttg tggttgacat gcatcaacaa
    28801 aggaagcctt ttagcttcct cgttgtgcaa tagatcaccg ttttttcggc cgactggcgt
    28861 acagacagaa gagaatggag ctggttgcgc agaatgcaat tgaccaaatc atcggccatg
    28921 cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat tgcccctatg ccaaaacgga
    28981 aggttcctgc cagcgaagat gccgttcccg ccatatgggg aaactcatca agaatgaccg
    29041 ccatcgcatt ggatgacacc atcgacacgc agcccacaaa cgccgcaacg ccaaccacca
    29101 gcgaccagaa tcccagcccc agtagcgcac tgatgaccat ccacgctgcc ataataaact
    29161 gtatccacaa ccccgagcgg aacatattta acgcgccaat acggcggacg aagcggctat
    29221 taaagatggt catcacgaac aggaaaacaa tgtttagtgc aaagtaataa ccaaagtttt
    29281 ccggcgcgac gtggttaatt tcaatgtaga caaacggtcc ggcgcttaag aacgagaaca
    29341 tcccggcaaa gctgaaacca ctggccagca tataactcag gacacgctta tgacggaaca
    29401 gcgccgcaaa gttaccaata gtggtacgaa tgtgaaaagg ctggcgacgc tccggtggta
    29461 aggtttcttt gatcagaaag aagatcattg ccgaagccag aatcgccgcc acggcgagga
    29521 tccagaagat gtaatgccag ctcagccaca ccagcaccca gccgccaact atcggtgcca
    29581 tcagcggtgc aatggttgtc accagcatga caaacgacat catccgcgag aactcttctt
    29641 tagggtagat atcacgcatc aaagcgttga taaccacgct ggccgcagcc gccgccagcc
    29701 cgtggaagaa acgcatcaca atcagctgat cgatggtttg tgccaacgca cacgccaccg
    29761 cggcggcggc aaacaccagc gtaccgccaa gaaccaccgg cttacgcccg aagctgtctg
    29821 ccatcggccc gtagattaac tgccccaacg caaagcccag aatataagta ctgagggtca
    29881 tctgcgtact gcccgccggt acgccaaact gcgctgaaat taccggtagc gcgggcagat
    29941 acatatcaat cgacagcggc atcaacatgg ccagcaggcc aaggataaaa acaatagcaa
    30001 acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa gtcattgaaa agttagacga
    30061 cgctggcaat ttcttcttca gttaacggac gatattcacc gggggctaaa tcagcatcca
    30121 gcgtaatgcc gccaatacgt tcacgatgca gctcaaccac atggttaccc acggcggcga
    30181 acatgcgttt cacctgatga taacgccctt cgctgatggt cagacgaacc tgcgttgggg
    30241 taatcacttc cagcaccgcc ggcttagtga gatctttttc gttatgcagc tgcacgcctt
    30301 tagcaaattg ctctgccgta tcgtcagcta cgggcgattc cagcgtcacc agataggtct
    30361 tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca ctgaccatca tctgtcatta
    30421 gtaccagacc ggtggtatcg atatccagcc gccctgcggc gtgcagtttc cacgccactg
    30481 gttcatccag aaaatagagc accgttgggt gatcagggtc gtccgtggag caaacatagc
    30541 cctgaggctt attgagcatg aagtaacgtg gaccgtgttg ctgcgccagc gggttgccat
    30601 cataagcgac atcatgttca ggaagcagtt tgaacgctgc attacggacg atttcgccat
    30661 cgacggtgac acgattgccg cggatttcac gcccggcaat agcacggcta acgccgagtt
    30721 gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt gcctgtaaaa acggaggtcg
    30781 ggcattgacc cgaaaatctg aactgttgtc tgcccagtat agcggtctaa taacttccct
    30841 caagggaaaa agaaccgtgg catactatta gcagaataat ctacctacgc gagaccatga
    30901 tttttacact tcgcccatat cagcaagaag ccgtggatgc cacgctcaac cattttcgtc
    30961 gtcataaaac ccctgccgtt atcgtgctgc ccaccggtgc aggtaaaagc ctggtgatag
    31021 cggaacttgc gcggctggct cgtggtcgcg tactggtgct ggcacacgtt aaagaactgg
    31081 tggcgcaaaa ccatgcaaag tatcaggcgc tggggctgga agccgatatt tttgccgccg
    31141 ggctaaaacg caaggagagc cacggtaaag tggtatttgg cagcgtgcag tcggtcgccc
    31201 gtaatcttga tgcctttcag ggtgaatttt cgctgttgat tgtcgatgaa tgtcaccgta
    31261 ttggtgatga cgaagagagc cagtatcagc aaatcctcac tcacctgaca aaagtgaatc
    31321 cccacttacg cctgctgggg ctgactgcca cgccttttcg attgggcaaa ggctggatct
    31381 accagttcca ttatcacggc atggtacgcg gcgatgagaa agcccttttc cgtgactgca
    31441 tttatgagct gccgctgcgt tatatgatta aacacggcta tctgacgccg ccagaacgac
    31501 tggatatgcc agtagtgcaa tacgatttca gccgcttgca ggcacagagt aacgggctgt
    31561 tcagcgaggc cgatctcaac cgcgagctaa aaaaacaaca acgtattacc ccgcacatca
    31621 tcagccagat tatggagttt gccgaaaagc gtaaaggagt gatgattttc gccgcgacgg
    31681 tcgaacacgc aaaagagatt gtgggattac tacccgccga agatgctgca ctgattactg
    31741 gcgacacccc cggcgctgag cgcgatgtgt taattgaaga ttttaaagcc cagcgttttc
    31801 gctatctggt caacgtcgca gtactgacca ccggatttga cgccccgcac gtcgatctta
    31861 tcgccattct gcgccctacc gaatcagtga gtctttacca acaaattgtc gggcgcggtc
    31921 tgcgtctcgc tccgggcaag actgattgtt taattcttga ttatgcgggt aatcctcacg
    31981 atctctacgc gccggaagtt ggtacaccga aaggcaaaag tgacaacgtt ccggtacagg
    32041 ttttctgccc tgcctgcggt tttgccaaca ccttttgggg gaaaacgacc gccgacggga
    32101 cattgattga acactttggt cgccgctgtc agggatggtt tgaagatgac gacggtcatc
    32161 gcgaacagtg tgacttccgt ttccgtttta aaaattgccc gcaatgtaac gcggaaaacg
    32221 atattgccgc ccgccgctgc cgcgaatgtg acaccgtact ggttgatcca gatgatatgt
    32281 taaaagcgga gctacgactg aaagacgcgc tggtattacg ctgtagcggc atgtctttac
    32341 agcatgggca cgacgagaaa ggcgaatggt tgaaaatcac ctattacgat gaagacggcg
    32401 cggatgtgag tgagcgtttc cgtctgcaaa cgcccgccca gcgaactgcc ttcgagcagc
    32461 tttttatccg cccgcatacg cgcacaccgg gcatccctct gcgctggatc accgccgccg
    32521 atatcctcgc ccagcaagcc ttattgcgac acccggattt tgtcgttgcc cgcatgaaag
    32581 gtcagtactg gcaagtgcgt gaaaaagtgt tcgattacga aggtcgtttt cgtcgggcgc
    32641 acgaattacg cggttaatgg tagttttgat tgatgtataa ggcgattgag tatagaatct
    32701 cgcccgcttt tgcatacgca aagcagatca cttacctgtt gctgggtcgc ctgtagcagg
    32761 attaatttaa gagagaaaga aatgtttact atcaacgcag aagtacgtaa agagcagggt
    32821 aagggtgcga gccgccgcct gcgtgccgct aacaagttcc cggcaatcat ctacggtggc
    32881 aaagaagcac cgctggctat cgagctggat cacgacaaag tcatgaacat gcaagctaaa
    32941 gctgaattct acagcgaagt tctgaccatc gttgttgacg gtaaagaaat caaagttaaa
    33001 gctcaggacg tacagcgtca cccgtacaaa ccgaagctgc agcacatcga cttcgttcgc
    33061 gcttaattgc tgaataagtt gtaaaaaacc ccgctccggc ggggtttttt gtatctgcag
    33121 attatgcctg attacgatat tgctattttt tgcaggccag ataaggcgtt cacgccgcat
    33181 ctggcattca taacacaaaa ccgattaatt gccgccggac gtccggcgct gcaattgatc
    33241 gcgcaaattc ggcggcgtgc ctttaatagt cagggtatcg gtcgccggat cccagaaaat
    33301 ccgttcgccc aacagcatcg catcaaaatt aatagtcaag ccaccgccgc taccggcaaa
    33361 cttcgtcaac tgacgcagtg tgctacggtc tgccgggaag ctctcttcca gttcgtagcc
    33421 tttttcagcc gcaaactccg tgaagctcac ttcgctgacg cctgccagct ctttcgacag
    33481 cgattccagt tcgatctctt cgcctgcctg cagctgctca ttacagtagc tataaacctg
    33541 ctggcacacg ttctgtcgct ctgctttatc cagttgtgct tcggcagtga aatcatcaac
    33601 cgcttgcaac agcccacggt tttgcgcttt cgcgttcaat ccttcgctgg ccccgaggaa
    33661 atccataaag aaatccgcca ctttgcgccc tactctccct ttcaggaaag tgagatagcg
    33721 ggtcgactct ggattggttt cccattcggt taaatcgata cgcgcaacaa tatccgcatg
    33781 attgatatca agataatggg ttgggttgat gtcgagattt tcgttaacgc gcatactgct
    33841 caggttgctc agaactgcca ccagcaaata ctccaccgcc agatagcgat agtggcaaaa
    33901 cagcacgaat ccaccgtcag caaaaggata tttcgctaat tcgtcacgca ggcgaccggt
    33961 tgccgcgcgg ctaaatgcca gaaagtcctc ttcaccctga cgctgtaagc gcagcgtctg
    34021 cgccagttca ctctcttcgc taaacaagcc gtaagcttta tttttggcgc tatagacccg
    34081 atgcagttct gctaccatct caacgacggt ttctgtcggt tccagcaatg aatcgcgcaa
    34141 caccagctca aggttctgct catcacgctt gataagctgg tgcagagcaa tctggttgat
    34201 atccagactc atgataaact ctcctttaag accgggcggt attcaaccac cgccggttaa
    34261 gcgacgcaat aaaagatggc ggtcatttta cagaaggcgg cgtaacgaga atgatttaac
    34321 gcataaaaaa gcagaaaaaa aaccgttgct acggtaatat gttgcccttt catcaactaa
    34381 ctgatttcga tttatgccac aaatttcccg ctacagtgat gaacaggttg aacaattgct
    34441 cgcagagctg ctcaacgtac tggaaaaaca taaggctccg accgaccttt ccctgatggt
    34501 gttaggcaac atggtcacta accttatcaa caccagcatt gccccggccc aacgccaggc
    34561 aattgccaac tcttttgccc gcgccttaca gtcctctatc aacgaagaca aagcgcacta
    34621 agggaaacag ataacaggtt atggtaactc atcgtcagcg ctaccgtgaa aaagtctccc
    34681 agatggtcag ttgggggcac tggtttgcac tgttcaatat tctgctttcg ctcgtcattg
    34741 gcagccgtta cctgtttatc gccgactggc cgaccacgct tgctggtcgc atttattcct
    34801 acgtaagcat tatcggccat ttcagcttcc tggtgttcgc cacctacttg ctgatcctct
    34861 tcccgctgac ctttatcgtc ggctcccaga ggctgatgag gtttttgtcc gtcattctgg
    34921 caacggcggg aatgacgcta ttactgatcg atagcgaagt ctttactcgt ttccatctcc
    34981 atcttaatcc catcgtctgg caactggtta tcaacccaga cgaaaatgag atggcgcgcg
    35041 actggcagct gatgttcatc agcgtgccgg ttattttatt gcttgaactg gtgtttgcga
    35101 cgtggagctg gcaaaagctg cgcagcctga cgcgtcgtcg gcgcttcgcg cgcccgctgg
    35161 ccgcattctt atttatcacc tttatcgcct cgcatgtggt gtatatctgg gccgatgcca
    35221 acttctatcg ccctatcacc atgcagcgcg ctaacctgcc gctttcgtac ccgatgacgg
    35281 cgcgacgttt tcttgagaag catggcctgc ttgatgcgca ggagtatcaa cgccgtctta
    35341 ttgagcaagg taatccagac gccgtttccg ttcagtatcc gttaagcgaa ctgcgctatc
    35401 gcgatatggg cactgggcag aatgtgctgt tgattactgt cgatggcctg aactactcac
    35461 gcttcgagaa gcagatgcct gcgctggcag gttttgctga gcaaaatatt tcgttcacgc
    35521 gccatatgag ctccggcaac actacagaca acggcatctt tggcctgttc tatggcatct
    35581 cgccgagcta tatggacggc attctgtcga cccgtacgcc tgcggcatta attactgcgc
    35641 ttaatcagca aggctatcag ctggggttat tctcatcaga tggctttacc agcccgctgt
    35701 atcgccaggc attgttgtca gatttctcga tgccgagcgt acgcacccaa tccgacgagc
    35761 agaccgccac gcagtggatc aactggctgg gccgctacgc acaagaagat aaccgctggt
    35821 tctcgtgggt ctctttcaat ggcactaaca ttgacgacag caatcagcag gcatttgcac
    35881 ggaaatatag ccgggcggca ggcaatgtcg atgaccagat caaccgcgtg ctcaatgcac
    35941 tgcgtgattc tggcaaactg gacaacacgg tggtgattat cactgccggt cggggtattc
    36001 cactgagcga agaggaagaa acctttgact ggtcccacgg tcatctgcag gtgccattag
    36061 tgattcactg gccaggcacg ccggcgcagc gtattaatgc gctgactgat cataccgatc
    36121 tgatgacgac gctgatgcaa cgcctgctac atgtcagcac acctgccagc gaatattcgc
    36181 aaggtcagga tttgttcaac cctcaacgcc gtcattactg ggttaccgca gcggataacg
    36241 atacgctggc aattaccacc ccgaaaaaga cgctggtgct gaacaataac ggtaaatacc
    36301 gcacttacaa cttacgtggt gaaagagtga aagatgaaaa accacagtta agtttgttat
    36361 tgcaagtact gacagacgag aagcgtttta tcgctaactg attaattata aatcagttag
    36421 cgaaatatct tacttgcaat cggtgtggaa aacggtagta ttaacagcca cgagtcggca
    36481 cgtagcgcag cctggtagcg caccgtcatg gggtgtcggg ggtcggaggt tcaaatcctc
    36541 tcgtgccgac caaattgccc accggatatc accggaaagc accgaataag acagggataa
    36601 cccgctaatc acttgattgg cgggtttttt tatggttttt agtcaccgga tatcaccgga
    36661 tttataagct atcagccgga ttttttagtt atacgcttag ttatacgggc tggcgtataa
    36721 ctaaagccgt ataactaaaa ccctgttttt tcgtgtggaa tcatagacat atggcgagaa
    36781 aaacacaccc attaaccaca gtgcagatca aagcagccag accagcgcaa aaggagtaca
    36841 ccctgcagga cggcggaggg ctttttctct tggtcaaacc gtctggatca aaactctgga
    36901 gattttccta ctaccgacca tcggacaaaa aaagaatatt gctgagtttt ggatcgcttg
    36961 aagatgtttc cctggctgat gccagaaaac gccgtagcga gtacaggacg ttaatcagtg
    37021 ccggaactga cccgcaggac cacgagaggc aaaaaagaga gacagaggcc cgaagacaag
    37081 ggaacacgtt cgaaaatgtg gcggcggcat ggtaccaggt gaaaatcagc cagaatctgg
    37141 cccccaacac gattaaagac atctggcgtt cgctggaaaa atatgtattc ccgttcatcg
    37201 gcaacacgcc aatagacacc ctgaccgctc gcaggttcgt tgaggtgctt acccccatca
    37261 aggaacgtgg caacctggaa acactaaagc gggttttgca gcgaattaat gaggtaatgg
    37321 attacgccgc caacagtggg ctgattgata ccaatccggc tatgaatgtg cgtaaggcgt
    37381 tcccctcccc tgtaaaaaaa catatgccaa caatccgccc cgaacagttg ccggagctta
    37441 tgcaggcttt atcagtatcg gcaacagaac ggcagaccag attactgatt gaatggcagt
    37501 tactgactgt aacccgtccg gttgaagcat cgtcagcacg ctgggaagaa atcgacctgg
    37561 acgcgcaaca atggacgata cccgccggac gcatgaagat gcggcgtgat cacgttatcc
    37621 cgctttgtag tcaggccatg gcggtactgg aggccatgaa accaatcagc caccaccgcg
    37681 attatgtttt cccgagcctg aaagacccac ggcagcctat gaacagccag accgcaaacg
    37741 cagcattgcg gcggatgggg tttggcggga ttctggtttc tcacggtatg cgcgccatat
    37801 tcagcaccgc agcgaacgaa gaaggatttg agccggacgt aatcgaggcc gcactagccc
    37861 acgtggacac caacgaagtg agacgggcat acaaccggag caactacata gaaaaacgca
    37921 ttgtgcttat gcgctggtgg ggcgaatttg tcgaggcggc ggcaacgggc gtaacccttg
    37981 ccagtggtaa aaggggtatt cgggccgtgt agctgtacag aaaaccagta aaaactacga
    38041 aaaccatgca aaaccgtcgt ataattgcac caaatttaac gacaaggccg cgaaacatga
    38101 aaccgttaag atgcaaaaaa atatcagatg caattgcgac gggctgcaac tggccctgat
    38161 ggttcagcat gaattttggt caacctacga tccggaggac agaacgacag ccccatcaaa
    38221 aaaacaggta gtagattttc tggtatcccg cggggcttcc agaaatctgg cggtaagtat
    38281 tgatagggta gtacgtccgg catccatgaa aaccggaggc aggccaagaa agtggcgtta
    38341 acactccctg aaagcggcag aaatgccgct ttttttataa ttccctttca aatcatcaac
    38401 ataaaaaacg gttataaccg tttaaaaacg gtgggaactg tttttaccca tattcgatga
    38461 tttaccgtat ttgtcaccgg aatacaccgg attcacaagg taaatcacga tggaagcaat
    38521 caaaaaagtc atctttcgcc aggaagtaaa aaaacttatc cacatcaagg cagacagcac
    38581 gctgcaaagc atgatcaacg ccggagaatt cccgcagggt tttcgtgttg gtttacgccg
    38641 tcggggatgg tatgaggatg acgtaatcgc ctggatgaaa gaacgcgaac aggaagcgcg
    38701 cgtaacggct gcttaacggg tgtatctcat atgacgaaca cgaaaaaagc ggccccgata
    38761 tggagccgcc tttctgaaca attaacccgc tgcgcattat gtgtcaacaa acataagcca
    38821 gggcatgata gccgctatca ggctggtggg caatgctatc agtctgtttc agtgcgtttc
    38881 cataactgcg aagctctctc ctttgatgag tattctttaa ggtacttctc aagggcaaaa
    38941 gaacacggcg cgaatcgttc tgattcatgc tctttctttc tgcgtcgtct ttttcgtgca
    39001 ggtgatgttt ttttggttga ttcttcgttg gtcattgtgt gcgcctgtaa agcaatgcgc
    39061 cggagttcct cacaccacgg cgctgatagt ttttatcctt tgggttctat gccgcgttgc
    39121 tgtaattctt tgcggattat tcgctttatc caggcagcaa gagaatcatc gccgtcctgt
    39181 tgttgtgcct tttccatctt ttcccgtaag tcaggatcta aacggaactg gaacggagga
    39241 ttaccacgcc tttcgttttt gtgtgttgac acgttaatta cacctcatgt aatgtattta
    39301 tgtgtaatga cacattacac acaatcaaca caaaaagcaa agccccgcac tgtaggagca
    39361 gtaacggggc ttctaaccaa caacgtaaac taggagccgt tatggttgcc gtaaatcata
    39421 taccacacct tgtacacaca caaacggcct ttgtgtggcg ttttctggcc ctgagtgccg
    39481 gagaatctca aatcatccac gtaaccgcct ggacggaacg cgaagcgcgt agccgttgcc
    39541 cgtcaggttg tgttgctgta tttgccgctc gtattcgtca ggggtcacat catgcgtaaa
    39601 aaccgcttgc aaaaaattat cacggggctg tatgcttcac ccgtcgccca catggcgacc
    39661 gggtttgaca gcctgaatac atctggcgga cagccgccca tatccgataa gcggtttttt
    39721 tgtgtccgta aacctaccca tacccgcatt atggcggggc gtaacggggg agcctttgcg
    39781 ctcgctggtt tccagatgac cagtctgtca accctgttac gtctcgccac catgtttgac
    39841 agcgtagtag cgagactcct taaatttcat ctgggagcct ttcacatggc tgtattcgca
    39901 cgcccttact ttgtctggcg ctttatgcag tgccaccaca gcagcattga tatttacacc
    39961 gttaccgcag ccactgaacg cgaagcccgc gccaggttgc cgcacgcaca tctgattttt
    40021 gtcgcccgta ttcgtcaggg gaaaacctat gcacaataaa accacaccgg acgcagcagc
    40081 cgccgcgctc actacgctga tgcacgcact tattgatatt gaatgcacag cagggcttgc
    40141 acaaaaggaa gaacgggaag aatacacatt gttcgccctg gaatgtatcc gatacaccgc
    40201 cacgcggtcg ctgaatgacg ctaaaaatat tcttattgct gattgtgaaa atgggggggg
    40261 ttatgcgtga tgatcgtttt aattccctga aacaggaatt ttccggcgta tctgatgatg
    40321 cggctgatgc gctttctgca atatccgaac tgataagagc ggcctttttc ttacttggca
    40381 cgaaagaaaa taaaacaacg gacggtaatt aatatgcgtg atatttacct cgaaacaata
    40441 gaccgcgcat ttcttgcact ttctcacagt gaaatcatga tggaaatgtt gcgcatatgg
    40501 cttgaaacgc ttggcgataa tgaacgagac aaacaaaaat cacggattgc cacggcatta
    40561 ataactctac ttgagcctgt aataatggaa ctgcaagaaa tagatctatt gcacgacaga
    40621 tataaagaac agcacaccgg agaataaaaa taatgaaact taaatattct ggcttaactg
    40681 ccagtggcaa cactcaccct aaatttacgc gcggtgatat ttaccgcgac cagtacggcg
    40741 gcacggtaat gattaagggc gtggcgggac ggtgcgtaac ttaccgccgt gaaggttacg
    40801 aatatgattg cgtgatgcct gtttatcagt tccggcgtga tttttctctg gtacagaccg
    40861 cgccgcataa cgtgcccacc agcaacgcca gggcacgggc aaacatccag aagctgaaaa
    40921 ccatgattaa cggattcagg ggtaaaaaat gaaactggca ccgaatgtaa aactgttacc
    40981 gaaagataaa cacaccgaag cggttatttt tgcgggtgat aatgctcact catttgcaga
    41041 gcactacatc attgcacagg ccaaaaaagc aggcgatcca gtccctcccg tttacctggg
    41101 gcgttatcag ttaagcgaac tggataactt gcagattgtt gataaaggtc ggttcagggc
    41161 gaaagtgata cgtgccggaa atattgacga agtgcagttg ttaaccatcg ccacgaaact
    41221 ggcaatcgca ggagtccagg aagcgcgcct gttttctgaa aatttcgaat tgctggagga
    41281 atggagcgac cagcttccac ggcttaggga gacatgggaa cgcggggaaa gcctgagcca
    41341 ccagcgaaaa accacgctac cgatgagcgt tggatctgcc ggatacgaca cgcaattaga
    41401 ttacgtggta aaggggatta ttccggcatc gtcgctatgc agcatatacg gggcgagcgg
    41461 ttcatataag tcgttccttg ctggttcgtg ggcgtgtcac gtttcaacgg gtcgccactg
    41521 gggaggccgc agggttgcac atggtgcggt cctctatgtg gttggtgaag gcggtattgg
    41581 tgtaccgcgt cgcgtgagag cgtgggaagt tgtgcacggt gagcaggtga aaaatctgta
    41641 cctggtaaac cgtcccatct ttccggctgc cccgtttgat gttgatgaaa tggttatcgc
    41701 tgcccgtcag gtggagcggg aaacgggtaa acctgtacgc atgattattc tggatacgct
    41761 ggcgcgttgc tttggtggta atgatgaaaa tgattcccgt gatatggggg cgtttatccg
    41821 tggttgtgac gaactgaaac ggcgcacagg ggccacggtg ctggtggttc accattccgg
    41881 caaggatgaa acaaaggggg cgcgtggttc cagtgcattt cgtgcatcac tggacgctga
    41941 ataccggata cgcagggagg acgcaggcag cgaagcgctg gttatctcat gcaccaaaat
    42001 gaaggacgcg gaggaactca aagaagccgc atatgactta cgcgtggtgg agctttttac
    42061 cgacgctgac ggggagttaa tcacgtcgct ggtggtggtg gataagccgc gccctcccgt
    42121 tgaactggag cgcatcgagg aggcaggcaa caagacggaa aaccataccg cgctatgggg
    42181 gtgcatccgt tcacgtacac agaacggcga caagtgcacg atcccgctgt tacgtgatga
    42241 catgaaacgg cttgggtatg acgtgaaaaa tatgcggcga tggttagcca aactggaaaa
    42301 agacgccgtg atttacattg atggtgatga tgtaggacca ctgtaaaaag tgagtagcaa
    42361 aagtgagtaa tggtgaggtt tttacaaaat attaacacga ttgctcactt tctcacctgt
    42421 atacatgctc aaaagtgagg cgtaaaaaaa acacttatga aacatgcgca tacaaaacac
    42481 aaaaatctca agtgagacga agcgagacgc tttaaaaagt gaggcgtaaa agtgagcatg
    42541 agtgaggaga taaccaaaat gcgcagagac agaacagagc cgaaatataa agcgttagac
    42601 atgacagagc acgccttaaa ggtggcaatc agggtaatag accgccacgc aggggaagga
    42661 tacgcgaagg aacatcccga cctgataagc gcattcatga ccacagcagc ggcaaacttt
    42721 gccacgttga cagaacggga gattgccgaa gcggaacagg taacaaccat caacgttaaa
    42781 accggagagg tggaattatg acggcacaga tagccgctta cgggcggctg gtggacgacc
    42841 cgcaggtaaa acagaccagc aagggcacac cgatgacgct ggcgcgtatg gcggtatctt
    42901 tgccatgcag ccaggcacag gacggacagg cgacgttatg gttatcggtc atcgcatttg
    42961 gcaaacaggc cgacttcctg gctaaacatc aaaaaggcga tgttgccagc gtatccggca
    43021 cgatgcaggt aagccagtgg accggacaga acggagaaac gcggcagggc tggcaggtta
    43081 tcgcagacag cgtaatcagt gcccgcgcgg cacgtccagg cgggaacaga cgcaaaacca
    43141 caggcacaca gggtaatcag ccaccagcgg gaggcgatga cccttacggt gatgatattt
    43201 cgttctgagg gggtgacgat ggtacatgac cgcatagcgg aggaactgga ggcgaaaggc
    43261 ttttaccgga gggcggcggc gcgatggggt gaagtcatgc agctggtgga gacagacaag
    43321 gaacggcatc acatcacgat gcgacggctg gaatgttcaa ggaaggcaca gagggcaccg
    43381 gagccgccgg ataatttcgg agacctgaaa aaggcagtcg atcgcactta tgccgaaatg
    43441 ggtatagatg gtgctggtga tgaaatatgg cgcaattacc aagacagcta atcaaacagc
    43501 cggagaaatc cggctttttt tgcaccagtt gaaacggtat ggcgcattac cgggttttcg
    43561 tcacggtcag gcatagttac tatctgaaac aaacagacac aacagagg
//
LOCUS       DAZFKF010000032        43564 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFKF010000032 DAZFKF010000000
VERSION     DAZFKF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.032"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            80..841
                     /gene="adiY"
                     /locus_tag="ACHKA1_002170"
     CDS             80..841
                     /gene="adiY"
                     /locus_tag="ACHKA1_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO5679823.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCKNNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCISDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            978..2315
                     /gene="adiC"
                     /locus_tag="ACHKA1_002171"
     CDS             978..2315
                     /gene="adiC"
                     /locus_tag="ACHKA1_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO5679824.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2419..4062
                     /gene="eptA"
                     /locus_tag="ACHKA1_002172"
     CDS             2419..4062
                     /gene="eptA"
                     /locus_tag="ACHKA1_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO5679825.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4059..4727
                     /gene="basR"
                     /locus_tag="ACHKA1_002173"
     CDS             4059..4727
                     /gene="basR"
                     /locus_tag="ACHKA1_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO5679826.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4728..5828
                     /gene="pmrB"
                     /locus_tag="ACHKA1_002174"
     CDS             4728..5828
                     /gene="pmrB"
                     /locus_tag="ACHKA1_002174"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO5679827.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(5804..5893)
                     /gene="pmrR"
                     /locus_tag="ACHKA1_002175"
     CDS             complement(5804..5893)
                     /gene="pmrR"
                     /locus_tag="ACHKA1_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO5679828.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(6005..7507)
                     /gene="proP"
                     /locus_tag="ACHKA1_002176"
     CDS             complement(6005..7507)
                     /gene="proP"
                     /locus_tag="ACHKA1_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO5679829.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7771..8649)
                     /gene="rdcB"
                     /locus_tag="ACHKA1_002177"
     CDS             complement(7771..8649)
                     /gene="rdcB"
                     /locus_tag="ACHKA1_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO5679830.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWANGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIVALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGRDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLV"
     gene            complement(8646..10874)
                     /gene="crfC"
                     /locus_tag="ACHKA1_002178"
     CDS             complement(8646..10874)
                     /gene="crfC"
                     /locus_tag="ACHKA1_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO5679831.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQRAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQMLLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQIKMELS
                     ESGFRPGFHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            11276..11611
                     /gene="yjdM"
                     /locus_tag="ACHKA1_002179"
     CDS             11276..11611
                     /gene="yjdM"
                     /locus_tag="ACHKA1_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO5679832.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            12069..12512
                     /gene="yjdN"
                     /locus_tag="ACHKA1_002180"
     CDS             12069..12512
                     /gene="yjdN"
                     /locus_tag="ACHKA1_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO5679833.1"
                     /translation="MPLSPYISFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            12645..13433
                     /gene="phnC"
                     /locus_tag="ACHKA1_002181"
     CDS             12645..13433
                     /gene="phnC"
                     /locus_tag="ACHKA1_002181"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO5679834.1"
                     /translation="MQTIIRVEQLSKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLIVGDKKPECRVELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            13458..14474
                     /gene="phnD"
                     /locus_tag="ACHKA1_002182"
     CDS             13458..14474
                     /gene="phnD"
                     /locus_tag="ACHKA1_002182"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5679835.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLSDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISANDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            14580..15359
                     /gene="phnE"
                     /locus_tag="ACHKA1_002183"
     CDS             14580..15359
                     /gene="phnE"
                     /locus_tag="ACHKA1_002183"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO5679836.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            15380..16105
                     /gene="phnF"
                     /locus_tag="ACHKA1_002184"
     CDS             15380..16105
                     /gene="phnF"
                     /locus_tag="ACHKA1_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO5679837.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLALWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            16106..16558
                     /gene="phnG"
                     /locus_tag="ACHKA1_002185"
     CDS             16106..16558
                     /gene="phnG"
                     /locus_tag="ACHKA1_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO5679838.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            16555..17139
                     /gene="phnH"
                     /locus_tag="ACHKA1_002186"
     CDS             16555..17139
                     /gene="phnH"
                     /locus_tag="ACHKA1_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO5679839.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            17139..18203
                     /gene="phnI"
                     /locus_tag="ACHKA1_002187"
     CDS             17139..18203
                     /gene="phnI"
                     /locus_tag="ACHKA1_002187"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO5679840.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDESPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            18196..19041
                     /gene="phnJ"
                     /locus_tag="ACHKA1_002188"
     CDS             18196..19041
                     /gene="phnJ"
                     /locus_tag="ACHKA1_002188"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO5679841.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            19038..19796
                     /gene="phnK"
                     /locus_tag="ACHKA1_002189"
     CDS             19038..19796
                     /gene="phnK"
                     /locus_tag="ACHKA1_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO5679842.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            19907..20587
                     /gene="phnL"
                     /locus_tag="ACHKA1_002190"
     CDS             19907..20587
                     /gene="phnL"
                     /locus_tag="ACHKA1_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO5679843.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            20584..21720
                     /gene="phnM"
                     /locus_tag="ACHKA1_002191"
     CDS             20584..21720
                     /gene="phnM"
                     /locus_tag="ACHKA1_002191"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO5679844.1"
                     /translation="MIINNVKLVLENEVVHGSLEMQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            21720..22277
                     /gene="phnN"
                     /locus_tag="ACHKA1_002192"
     CDS             21720..22277
                     /gene="phnN"
                     /locus_tag="ACHKA1_002192"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO5679845.1"
                     /translation="MTGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYASALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            22264..22698
                     /gene="phnO"
                     /locus_tag="ACHKA1_002193"
     CDS             22264..22698
                     /gene="phnO"
                     /locus_tag="ACHKA1_002193"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO5679846.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            22700..23458
                     /gene="phnP"
                     /locus_tag="ACHKA1_002194"
     CDS             22700..23458
                     /gene="phnP"
                     /locus_tag="ACHKA1_002194"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO5679847.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPHYRRQPCSGVV
                     KFNEAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDTIPVYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNEVIRSPRV
                     ILTHISHQFDAWLMENALPPGFEAGFDGMEIGVA"
     gene            complement(23535..23864)
                     /gene="yjdP"
                     /locus_tag="ACHKA1_002195"
     CDS             complement(23535..23864)
                     /gene="yjdP"
                     /locus_tag="ACHKA1_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO5679848.1"
                     /translation="MKRHSTLFFFTLLTLTTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(23933..26194)
                     /locus_tag="ACHKA1_002196"
     CDS             complement(23933..26194)
                     /locus_tag="ACHKA1_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5679849.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDDILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDMPLVNTL
                     LNDFNDEMRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSGQRV
                     AQSYQDLRSGERVIMIFALLAVVITAFAGWYIYRNLGTNLTAISRAMSRLAYGEADVT
                     FPALQRRDELGELARAFNVFARNTESLERTSRLLKEKTTQMEIAQTERQGLEEALLHS
                     QKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNPDSPDAPRIQRALQAAERGALLTQRL
                     LAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLALEIEAQSPAWPAWIDISQLENA
                     IINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGSGMSQAVKSQV
                     FEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAAKNL
                     SLAAVEQASVSGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAASAE
                     IDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLRKPF
                     TRAQLAQALGQEN"
     gene            26308..27828
                     /locus_tag="ACHKA1_002197"
     CDS             26308..27828
                     /locus_tag="ACHKA1_002197"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO5679850.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLSVAENI
                     FLGSELSHGGLVQRKEMLVQAQKVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHLLTDGGKVQPSSL
                     LVRSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLAIGF
                     LTENRKEQGLFLEMAAAENITMATLERDARWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVYVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            27825..28799
                     /locus_tag="ACHKA1_002198"
     CDS             27825..28799
                     /locus_tag="ACHKA1_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO5679851.1"
                     /translation="MTTSEKTSAKKILMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVVCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSASRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            28818..29759
                     /locus_tag="ACHKA1_002199"
     CDS             28818..29759
                     /locus_tag="ACHKA1_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5679852.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            29814..30497
                     /locus_tag="ACHKA1_002200"
     CDS             29814..30497
                     /locus_tag="ACHKA1_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO5679853.1"
                     /translation="MKRSAINDILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGRNNFAAHGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTADSDITVVIDGCRQKHTAGTQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFR"
     gene            30512..31372
                     /locus_tag="ACHKA1_002201"
     CDS             30512..31372
                     /locus_tag="ACHKA1_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HFO5679854.1"
                     /translation="MPLISLAEGLAHAREHHYSLGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARHAIPVVLNLDHGLHFESVVQALRLGFSSVM
                     FDGSTLGYEENVRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGISDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFCSAGQA"
     gene            31369..32370
                     /locus_tag="ACHKA1_002202"
     CDS             31369..32370
                     /locus_tag="ACHKA1_002202"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO5679855.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQWTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQGGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(32367..33074)
                     /locus_tag="ACHKA1_002203"
     CDS             complement(32367..33074)
                     /locus_tag="ACHKA1_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO5679856.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVCHTGQEAILRIEG
                     DPDIALVVLDMMLPDTNGLRVLQQIQKLRPTLPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRRVGALAVNDEKSAGLSFNGWYLDTMRCQLHNPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
     gene            33365..35350
                     /gene="yjcS"
                     /locus_tag="ACHKA1_002204"
     CDS             33365..35350
                     /gene="yjcS"
                     /locus_tag="ACHKA1_002204"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO5679857.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSEKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            35559..35834
                     /gene="ytcA"
                     /locus_tag="ACHKA1_002205"
     CDS             35559..35834
                     /gene="ytcA"
                     /locus_tag="ACHKA1_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO5679858.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRTNINLAFVGIIYTALFALYAMLFWLAFF"
     gene            35853..36884
                     /gene="mdtN"
                     /locus_tag="ACHKA1_002206"
     CDS             35853..36884
                     /gene="mdtN"
                     /locus_tag="ACHKA1_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO5679859.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            36884..38935
                     /gene="mdtO"
                     /locus_tag="ACHKA1_002207"
     CDS             36884..38935
                     /gene="mdtO"
                     /locus_tag="ACHKA1_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO5679860.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGVQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQVLNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICHTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATGSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLTVALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQVQGAT
                     Q"
     gene            38932..40398
                     /gene="mdtP"
                     /locus_tag="ACHKA1_002208"
     CDS             38932..40398
                     /gene="mdtP"
                     /locus_tag="ACHKA1_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO5679861.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKNSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVLQSIQLMKSLGGGYQAGPVVEKK"
     gene            40596..42743
                     /gene="fdhF"
                     /locus_tag="ACHKA1_002209"
     CDS             40596..42743
                     /gene="fdhF"
                     /locus_tag="ACHKA1_002209"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:41013..41015,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO5679862.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            42837..43526
                     /gene="yjcO"
                     /locus_tag="ACHKA1_002210"
     CDS             42837..43526
                     /gene="yjcO"
                     /locus_tag="ACHKA1_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO5679863.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
BASE COUNT        10132 a        12108 c        11738 g         9586 t
ORIGIN      
        1 atacaattat ataaccattt cccggtatcg ctttgcttta gcgagaactg gtcttttatt
       61 cgcactcagg agtacatgta tgaggatttg cagcgaccaa ccttgtattg ttttattgac
      121 tgaaaaagat gtctggataa gggtgaatgg gaaagaacct attagcctta aagctaacca
      181 tatggcgtta ttaaattgta aaaataatat tatcgacgtc tcctctctta acaacacttt
      241 ggttgctcat attagtcacg acatcatcaa agattacctc cggtttctga ataaagatct
      301 ctcgcaaata ccagtatggc aacgtagcgc tacgcccatt ctcaccctgc catgcctgac
      361 accagacgtc tttcgcgttg ccgcgcaaca cagcatgatg cccgcagaaa ctgagtcaga
      421 aaaggaacga acacgcgcat tattattcac tgtgctatcc cgttttctcg acagtaaaaa
      481 attcctttca ttaatgatgt atatgttacg taattgtata agtgacagcg tttatcaaat
      541 tattgaaagc gatattcaca aagactggaa tcttagtatg gtagccagtt gtttatgtct
      601 tagcccaagt ctgttaaaga aaaagctgaa aagcgaaaac accagttata gccaaataat
      661 caccacctgc cgcatgcgtt atgccgtaaa tgaattaatg atggacggta aaaatatctc
      721 tcaggtatca cagtcctgcg gctacaacag tacgtcgtac tttatttctg tctttaaaga
      781 cttctacggt atgacgccgc tgcattatgt tagtcagcac agagaacgca ctgtcgcctg
      841 atttttaacc ttaacgaaga gctatattaa taacggcatc agcgataacc cggtcgataa
      901 taattcaact atcgaatgca ggcgtatgat atgacgtaat ttattgtcac gaagctcgcc
      961 ttcgcaggag tttaattatg tcttcggatg ctgatgctca caaagtgggc ttaatccccg
     1021 tcaccctgat ggtgtcgggg aatattatgg ggtcaggtgt ttttctgtta cctgcaaacc
     1081 tggcctccac tggcgggatt gccatttatg gatggttggt gacgattatc ggtgcgctgg
     1141 ggctctcgat ggtatacgcc aaaatgtcgt tcctcgaccc aagccctggt ggttcttacg
     1201 cttacgcccg ccgctgcttt ggcccgtttc tcggttatca aaccaacgtc ctctactggc
     1261 tggcctgctg gatcggcaat atcgccatgg tggtcattgg cgtaggatat ttaagttact
     1321 tcttcccgat tctgaaagat ccattggtat taaccatcac ctgcgtcgtg gtgctgtgga
     1381 tcttcgtcct gctgaacatt gtcggtccga aaatgatcac ccgtgtgcag gccgttgcca
     1441 ccgtgctggc gctgatcccc atcgtcggga ttgccgtatt tggctggttc tggttccgtg
     1501 gtgaaaccta tatggcggca tggaacgtca gcggcctggg cactttcggt gcaattcaaa
     1561 gtacccttaa cgttacgctg tggtcgttca tcggtgtgga aagtgcctcc gttgccgcag
     1621 gtgtggtgaa aaacccgaaa cgcaatgtcc ctatagccac cattggtggg gtattgattg
     1681 ccgccgtttg ctatgtgctc tccaccaccg cgattatggg gatgatccct aatgccgcac
     1741 tgcgcgtttc tgcctcgcca ttcggtgatg ccgcacggat ggcgttgggt gacaccgccg
     1801 gggccattgt ttccttctgc gcagctgcgg gttgcttagg ttcactgggc ggctggacat
     1861 tgctggcggg tcaaacggcg aaagccgctg ccgatgacgg actgttccca ccgatttttg
     1921 cccgtgtaaa taaagcgggt acgccagtgg cggggctgat tatcgtcggt attttgatga
     1981 ccatcttcca gctcagcagc atttcaccaa acgcgaccaa agaattcggt ctggtttctt
     2041 ccgtctcggt catctttaca ctggtgccat atctttacac ctgtgcggcg ttactgctgc
     2101 tcggacacgg tcactttggt aaagcacgcc cggcatatct ggcagttact accattgcct
     2161 tcctctactg catctgggcc gtggtggggt ccggggcgaa agaggttatg tggtcatttg
     2221 tcaccctgat ggtcatcacc gccatgtatg cgctgaatta caaccggctg cataaaaacc
     2281 cgtatccctt agatgcacca ataagcaaag attaattccc cttaatccag caaagataaa
     2341 agccaacctt aagaacttaa ggttggctta attttgcttt gcgagcatat gcgcactttg
     2401 ttcgatggaa acaccgtgat gttgaagcgc ctactaaaaa gaccctcttt gaatttactc
     2461 gcctggctat tgttggccgc tttttatatc tctatctgcc tgaatattgc cttttttaaa
     2521 caggtgttgc aggcgctgcc gctggactcg ctgcataacg tactggtttt cttgtcgatg
     2581 ccggtcgtcg ccttcagcgt gattaatatt gtcctgacac taagctcttt cttatggctt
     2641 aatcgaccgc tggcctgcct gtttattctg gttggcgcgg ctgcacaata tttcataatg
     2701 acttacggca tcgtcatcga ccgctcgatg attgccaata ttattgatac cactccggca
     2761 gaaagctatg cgctgatgac accgcaaatg ttattaacgc tgggattcag cggcgtgctt
     2821 gctgcgctga ttgcctgctg gattaaaatc aaacctgcca cctcgcgtct gcgcagtgtt
     2881 cttttccgtg gagccaatat tctggtttct gtactgctga ttttgctggt cgccgcactg
     2941 ttttataaag actacgcctc gttgttccgc aacaacaaag agctggtgaa gtccttaagc
     3001 ccctctaaca gcattgttgc cagctggtca tggtactccc atcagcgact ggcaaatctg
     3061 ccgctggtgc gaattggtga agacgcgcac cgcaacccat taatgcagaa cgaaaaacgt
     3121 aaaaatttga ccatcctgat tgtcggcgaa acctcgcggg cggagaactt ctccctcaac
     3181 ggctacccgc gtgaaactaa cccgcggctg gcgaaagata acgtggtcta tttccctaat
     3241 accgcatctt gcggcacggc aacggcagtt tcagtaccgt gcatgttctc ggatatgccg
     3301 cgtgagcatt acaaagaaga gttggcacag caccaggaag gcgtgctgga tatcattcag
     3361 cgagcgggca tcaacgtgct gtggaatgac aacgatggtg gctgtaaagg tgcctgcgac
     3421 cgcgtgcctc accagaacgt caccgcgctg aatctgcctg atcagtgcat caacggcgaa
     3481 tgctatgacg aagtgctgtt ccacgggctg gaagagtaca tcaataacct gcagggtgat
     3541 ggcgtgattg tcttacacac catcggcagc cacggtccga cctattacaa ccgctatccg
     3601 ccgcagttca ggaaatttac cccaacctgc gacaccaatg agatccagac ctgtaccaaa
     3661 gagcaactgg tgaacactta cgacaacacg ctggtttacg tcgactatat tgttgataaa
     3721 gcgattaatc tgctgaaaga acatcaggat aaatttacca ccagcctggt ttatctttct
     3781 gaccacggtg aatcgttagg tgaaaatggc atctatctgc acggtctgcc ttatgccatc
     3841 gccccggata gccaaaaaca ggtgccgatg ctgctgtggc tgtcggagga ttatcaaaaa
     3901 cggtatcagg ttgaccagaa ctgtctgcaa aaacaggcgc aaacacaaca ctattcacaa
     3961 gacaatttat tctccacgct attgggatta actggcgttg agacgaagta ttaccaggct
     4021 gcggatgata ttctgcaaac ttgcaggaga gtgagtgaat gaaaattctg attgttgaag
     4081 acgatacgct gttattgcag ggactgattc tggcggcgca aaccgaaggc tacgcgtgcg
     4141 atggcgtgac aaccgcgcgg atggcggaac aaagccttga ggcaggtcat tacagcctgg
     4201 tggtactgga tttagggtta cccgacgaag atggactgca ttttctcgcc cgtatccggc
     4261 agaaaaaata taccctgccg gtactgatcc tcaccgctcg cgatacgctg accgacaaaa
     4321 tcgccgggct ggatgtcggt gccgacgact atctggtgaa gccttttgcg ctggaagagt
     4381 tacacgcccg tatccgcgcc ctgctacgac gccataataa tcagggcgaa agtgagctga
     4441 ttgttggcaa tctgacgctg aatatgggtc gccgtcaggt atggatgggc ggtgaagagt
     4501 tgattctgac gcccaaagaa tatgctctgc tgtcacggtt aatgctcaaa gcaggcagtc
     4561 cggtgcatcg ggaaattctc tacaacgaca tctataactg ggacaatgaa ccctcgacca
     4621 acaccctgga agtgcatatc cacaatctgc gcgacaaagt gggcaaagcc cgtatccgca
     4681 ccgtgcgcgg ctttggctat atgcttgtcg cgaatgagga aaactaattg aatctgatgc
     4741 gttttctgcg ccgaccaata tcgctgcgcc aacggctgat attgaccatc ggggccattt
     4801 tgttggtgtt tgagctgatc agcgtcttct ggctatggca tgaaagtacc gagcagattc
     4861 agctgtttga gcaggcgcta cgcgacaatc gcaacaacga tcgccacatc atgcgtgaga
     4921 tccgcgaggc ggtcgccagc ctgattgtcc ccggcgtctt tatggtcagc ctgacgctat
     4981 ttatctgcta tcaggcggta cgccgcatca cccgcccgct ggcggagctg caaaaagagc
     5041 tggaagcgcg caccgccgac aacttaacgc ccattgccat tcacagcgcc accctcgaaa
     5101 tcgaagcggt ggtttcggcg ttaaacgatc tggtcagtcg cctgaccagc acgctggata
     5161 acgaaaggtt gtttaccgct gacgtcgcgc acgaactgcg aacgccactg gcgggggtgc
     5221 gtttgcatct ggaactgctg gcgaaaacgc atcacattga tgtagcaccg ttagtggcac
     5281 ggcttgatca gatgatggag agcgtctccc agctgctgca actggcgcgt gccggacagt
     5341 cattttcttc cggtaattat caacatgtaa aactgctgga agatgtcatt ctcccctcgt
     5401 atgacgaact cagtaccatg ctcgaccagc gacagcaaac cctgctattg ccagagagcg
     5461 ccgccgacat caccgttcag ggtgatgcaa ccctgctgcg gatgttattg cgaaacctgg
     5521 tagaaaacgc ccatcgttac agcccgcaag gcagcaacat tatgattaag ctgcaagaag
     5581 atggcggagc ggtcatggca gttgaagatg aaggaccagg tattgatgag agtaaatgcg
     5641 gggagttgag taaagcgttt gtacgaatgg acagccgtta tggcgggatt ggtctggggt
     5701 taagtattgt cagccgcatt acacagttgc atcacgggca gtttttcctg caaaaccggc
     5761 aagagacttc cggcacgcgg gcctgggtac ggctgaagaa agatcagtac gtggcaaacc
     5821 agatataaag aaagctgctg accaccagca cgctgaacac ggtagttaaa ctttcataaa
     5881 cacggttttt cattgcactc tcctccgtta acctggagga gagtatgcgc gtcagagatt
     5941 aagtgaacct taagagttca attggcctga taagacagcg ttacatcagg ccatccgttt
     6001 cagcttattc atcaattcgc ggatgttgct gcaccaagcg ggtacgtttc gcctgcaaat
     6061 cggcaatctc gtggtcaata tcatcgattt tctgctcgat attatcgtaa tgctcgacga
     6121 gaatttcctt cgcttcctgt atatctgacg ccgccggtgt tgcacctttc aacggacgat
     6181 ttgccgtctc tttcatggtt acgccggtga ttaaaccaac caccgccact accatcaggt
     6241 aataggctgg catcatcaga ttctgcgagc tttcgaccag ccaggccgcc aatgttggcg
     6301 tcagaccggc aaccagcacc gaaatattaa atgccgccgc cagcgcgctg taacggatat
     6361 gcgtcgggaa catcgctggc aaggtagaag ccataacgcc cgtaaagcag ttaaggatca
     6421 ccgccagcat cagtaacccg gcaaaaatca gaccgatgac gttactgtta atcagaataa
     6481 acgccgggat cgccaacaca aacagggcaa cactaccaag tagcacaaac ggacgacggc
     6541 caaaacggtc actcagcaag cccatcaccg gctgaacaaa cagcataccg atcataatgg
     6601 cgataataat cagcaccccg tggtcttcgg agtaatgcag gttatgtgac aaataactcg
     6661 gcatataggt cagcaacatg tagtaagtca cgttggtggc aattaccaga ccaatacatg
     6721 tcaacaggct gcgccagtgt ttggtggcaa tctctttaaa cgagactttc gggccatcct
     6781 gcaaaccttc gcggtcgccc tgttccagtt tatcgacatg ctgctggaac gccggagtct
     6841 cttccagcgc atggcgcagg taaagcccga taatccctaa cggcagagcg ataaagaacg
     6901 gaatacgcca gccccaatcg aggaagttct cttcgccgac aatggtcgaa attaacacca
     6961 ctacgccagc acccagcaca aacccggcaa tagaaccgaa gtccagccag ctgcccataa
     7021 agccacgttt acggtcaggg gagtattccg caacaaatat cgacgccccg gtatattcac
     7081 cgccgaccga gaaaccttgt gccatcttac agatcaacag cagaatcggt gcccaaatac
     7141 caatcgtgtc gtaggacggt attaagccaa tacagaacgt actgatcgac ataatcacaa
     7201 tagtaatagc gaggatcttc tggcgaccat atttatcgcc caacatacca aagaagagtc
     7261 cgccaagcgg tcgaatcaga aagggaacgg agaacgtggc aagtgcagca accatctgca
     7321 cgctggggtc agcccccggg aaaaaaactt tacctaatgc gtaagcaaca aaaccataaa
     7381 caccaaaatc aaaccattcc attgcattac ccagtgatgc tgcggtaatg gctttacgca
     7441 gtttaccgtc atcaataatg gtgacatcac gaagggtaat cggttttact tttttccttt
     7501 tcagcatagc tttcctcgca gagttggccc atttccgcaa acgccagaaa aatcagcgcg
     7561 acggccgcaa aaggggagcg ggaattgcag gatgcaatct ctgtaacaag cgtagcgggt
     7621 ttgcttacac cctccggtta aggaatgtac aatcaaacca gttgacgcct gaattgggca
     7681 gttaatgacc tttttaccct accagcgttt aaatttgtga tcaacttcac acatggattt
     7741 cgtttaggac tcattgatgt aactgattat ttacaccaaa tttacagaga agatttcctc
     7801 gagcattgcc cccgccagac gctgcgctga acagagtcgt ggcatcccca gcgcgacagt
     7861 ttgccagcgt tgggtcactg accagcaaac gggttgttta tctgccgtgg tccactttcc
     7921 cagccaactt aaaaaatcag catgatccct gcccgtcgga acggtcgaag aggcaaacca
     7981 ctgatggtaa tagtgacgcg tggtcggggc cgcattgata ctttctgcca gatgttgtgt
     8041 cgctttcacg cgcaaatcac ccagcaacaa cgccaccgct tgcgggcttc ccagacggca
     8101 ggcatcgcca aatgcccccc aacgcaactc ttcgagaacg ataaaacagc gcccaggcaa
     8161 cgaccaggcg ttatacgccc cagcacgcca gcgggtaaaa atttgctcgc tctgttcacc
     8221 cgcctgcacc agtaggcctc gttgcgttaa cgccttttcc ttccagcgac aacgggctga
     8281 aaaccactca tacaaggcga caatttgctg ggggagctcc gggaaagcag agtgctggcg
     8341 gtgatccatt ttttgcagca gcgccagatg ttgcgcgatc tgcgtcagtg ccagatgacc
     8401 gggatccagc atcgacgcca gtttttccac caatgtcaaa cgcaggctaa gcggcgcatt
     8461 taacattccg ccgttcgccc atgtctgaag ctgcacgcgc gcaagaagtt cctgcatcag
     8521 tcgttcacga aactgctgct gatgggcttg tggaaggcgg gcatcatcgc cctgcgccag
     8581 atccactaaa aagcgcggat gaaccgactc cagcacgcga ccggggccgt ccagtaacgt
     8641 cttggtcaat atcgttctgc cgtgaaaagt gtttgaatat catcgcgtaa cagctgggcg
     8701 tcggtgtaaa tccagccgtg agtcatcaca gtctgctgca attgctgctg catcagcctg
     8761 accaccgatt cattttgttg acgcagagcc agactttcgc gtaaacgcgt ctgtaattcc
     8821 gtcaaacatg aagcgaactc agcgaaaaaa gtattcatgc ctgccgtaac agattcatcg
     8881 acctgctctg ccagaacttt agccatttgt tggcaataga gatcgacttc tgcgcttaat
     8941 gctcgttgca acacactgta atcaaccgtt tctgtcgggg atttctcatt tccccgtccc
     9001 cagtcgggct gattcaacca gcgcgaaaaa gtctcacgca caacgcctaa acgcgtgctc
     9061 tgctcgtccg ttgcttcctg gcgcgaaatg acttcactga acagctggcg agtgttgaag
     9121 tggggaactg cgccgtgaaa aacaggaaaa tgaaacccag gacgaaaccc tgactcgctc
     9181 aattccattt tgatttgttg ctcaatgggg cgaataacat cggttaacac ccggcaaagg
     9241 gtggattcaa gctcggcaaa acgcagcgta aagtcgcgac tgatggtgtt ctgcgccgtc
     9301 tgtaacagtg tctcacagcg ggtacgaatc tcgcttaacg gctccgaatc atcctgaaac
     9361 aacgcggcta actgcgcatt cagcgcatct tgttgttgac gcagaaagtg gttggcggag
     9421 gtcagggcca gctcgatttc atgtttaatc tcgccgctca cctgcgcctg attgagttgc
     9481 aatagctgca aactttcttc gacctgatgg atattttgcc gcaattgttc acaagcgacg
     9541 tttaacccgt gcgcacgaaa atccaggtat tcccgcgcct gctgcgcgta attcaacagt
     9601 ttatgcgcgg cagatcgcag agcatacaac gaggcgttag cgtaagcggc atgaagcaac
     9661 atctgaattg gctgggcgaa cagcgaatct tcccacaact gatcggcagc atgacgaata
     9721 tgttcaaggt ccgccagatc ggcatgacgc cagcgcctgc cgagcgcggc atgggcaaaa
     9781 tcttccaccc agcgttgttg ctctggcgct ggtaacttac cgttgttggc taactcatgg
     9841 cgcgcccgat tcgccaggta gccccacatc gacgacaccg gaaatatctg ctgtggcgta
     9901 atacagcctt tcatcagcgt cccggaaatc agtgcccgca cctggtcggc gtcgtcactg
     9961 ttacgatcct gttgatcgaa cttattgacc agcacataca gcggcaccga ttgccccacc
    10021 gccaaaatcg cctcacggac ctcttcatcg gagatcgatt tcagttgcgt ataatccagc
    10081 accgccagta ccgccgaggc gcgtgccagc tgctggttaa gcattttttg cagatgcggt
    10141 tgcccggctt catttggccc gggggtatcc agtaacgtca attgaccggg ataactctcc
    10201 agccccgcca gatggacaaa ctccacttca atcacgggaa tatgctcaat ggcggcgtaa
    10261 gcagaaaaag gaaaatcgac gtccagcgcc ttcgccagtc gcactaaatc attcaaactt
    10321 ttcagacaat gaaaaatagg ctgggcaccc agataatatt tttcgaaagc gacgccattt
    10381 tcgatccgct gcataagcgc acgcatatct ttatctattt ccagcacatc ggtcagatgc
    10441 ttaatatcgc aatcacgcag gcgctgttgt aattgttgaa ttaaacaatc gattggcgcg
    10501 acatgtgaaa aatgcagtac cggttccttt tgcccgggcg tatggcgaat aagcgtcggc
    10561 aacgcagtca ttgggcgatt gcgattaggc agtacctccg taccaacaat ggcattaatg
    10621 gtggttgatt tccctgcttt catagtaccg acaatcgcca gcaccatttc cagtcgggaa
    10681 attttacgca actcattatt cagcatcgcg tgacgctcgg cgatattagg ctgactccag
    10741 ggtaaagccc gttgtggcgc atcgtctccg ggtacagaga gaggcatttt ttccagtaac
    10801 tgcaactgtt ggcgagaaag ctgtaacagg cgttcagcct cctgacttaa ctcatacagg
    10861 gtctgtgtgt acatagaaaa ttcttcctta aagcaaattt tattactttt atttagccag
    10921 attgtttttg agttctgttt tcggctttta taattactgc aagaaataat tttatattta
    10981 gagtgttgtt ttttattaga ataaataaca tcttccgata cgtttaaaac gtcagaaaga
    11041 taaaaatatc atgtgaataa aaaaaagaac aagtagagca ttaaccttat cttaaataat
    11101 aaatggaggc aaaaagatta ttttcttttt gcgtttcctt tcaaatgaaa acgatcgtcg
    11161 tctaaaatca gcagtacccc cgacaaactc agggattttg tgtataattg cggccttttt
    11221 cggcaatctg ccgttttttg gcgcttttgc cctgctgact tttgaggaaa tccacatgtc
    11281 attaccacac tgcccaaaat gcaactccga atacacctac gaagataacg gcatgtacat
    11341 ctgcccggaa tgtgcctacg aatggaacga cgcagaacct gcacaggaaa gcgacgagct
    11401 gatcgttaaa gatgctaacg gcaatctgct ggctgacggc gacagcgtta ccatcattaa
    11461 agatctgaag gtgaaaggta gctcttcgat gctgaaaatt ggcaccaaag taaaaaacat
    11521 ccgcctggtt gaaggcgatc ataacatcga ttgcaaaatc gacggttttg gtccgatgaa
    11581 actgaaatct gagtttgtga aaaagaactg attgtattgt gatcggtaag ccggataagg
    11641 cgctcgcgcc gcatccggca acggtgccag atgcctgatg cgacgcttgc gcgtcttatc
    11701 aggcctacaa attcccgcac catccgtagg ccggataagg cgtttacgcc gcatccggca
    11761 acagtgccag atgcctgatg cgacgcttgc gcgtcttatc aggcctacaa attcccgcac
    11821 catccgtagg ccggataagg cgtttacgcc gcatccggca acggtgccga ctgcctgatg
    11881 cgacgcttgc gcgtcttatc aggcctacaa attcccgcac cctccgtagg ccggataagg
    11941 cgtttacgcc gcatccggca acagtgccga ctgcctgatg cgacgctcgc gcgtcttatc
    12001 aggccgcctc tcatctgtat aaatttcgaa ctacacttaa ctggcttctc ttcactgagg
    12061 tcaccatcat gccgttaagt ccctacatct cttttgccgg taactgttcc gacgcgattg
    12121 cctattatca acgcacgttg ggcgcggaac tgctctataa aatcagcttc ggcgaaatgc
    12181 caaaatcagc gcaggacagc gccgagaact gcccttccgg aatgcaattt cccgataccg
    12241 ccatcgctca tgccaacgtg cgcattgccg gaagcgacat catgatgagc gatgccatgc
    12301 cgtcaggaaa agccagctac tccggcttta cgctggtgct cgattcgcaa caggtcgaag
    12361 aaggaaaacg ctggtttgat aaccttgccg ctaacggaaa aatcgaaatg gcctggcagg
    12421 aaactttctg ggcgcatggc ttcggcaaag tcaccgataa atttggcgta ccgtggatga
    12481 ttaatgtcgt caaacaacaa ccaacgcaat aatccgccgg gaggcccgcc ctcccgcact
    12541 gtcatcgaat tcccgttaac tcttcatctg ttagtcactt ttaattaacc aaatcgtcac
    12601 aataatccgc cacgatggag ccactttttt agggaggctg catcatgcaa acgattatcc
    12661 gcgtcgaaca actgagcaaa accttcaacc agcatcaggc gctgcacgcg gttgatctga
    12721 acattcatca cggtgaaatg gtggctctgc ttgggccgtc cggttccggc aaatccaccc
    12781 ttttacgtca cttaagcggc ctgattgtcg gcgataaaaa gccagaatgc cgcgtcgaac
    12841 tgctgggccg cactgtccag cgcgaaggcc gcctggcccg tgatatccgc aaaagccgcg
    12901 ccaacaccgg ctacatcttc caacaattca acctggtgaa tcgcctgagc gtactggaga
    12961 acgtgctgat tggcgcgctc ggcagcacgc cgttctggcg cacctgtttt agctggttca
    13021 ccggcgagca gaaacagcgc gcgttacagg cgctgacccg cgttggcatg gtgcattttg
    13081 cccatcagcg cgtttccacc ctctccggtg gccagcagca gcgtgtggcg attgcccgcg
    13141 cgctgatgca gcaggcgaag gtgattctgg ccgatgaacc catcgcctcg ctggacccgg
    13201 aatcggcgcg catcgtgatg gacaccctgc gcgacatcaa ccagaacgac ggtatcaccg
    13261 tggtcgtcac gctgcatcag gtggattacg ccctgcgcta ctgcgaacgc atcgtcgccc
    13321 tgcgtcaggg acacgtcttc tacgacggca gcagccaaca gtttgataac gaacgttttg
    13381 accatctcta ccgcagcatt aaccgcgtcg aagagaacgc gaaagctgcc tgacatcccc
    13441 atcattgagg aaaacgaatg aacgctaaga taattgcctc gctggccttc accagcatgt
    13501 tcagcctcag caccctgtta accccggcgc atgccgaaga gcaggaaaag gcgctgaatt
    13561 tcggcattat ttcaacggaa tcacagcaaa acctgaaacc gcaatggacg ccgtttttac
    13621 aggatatgga gaagaagctg ggcgtgaagg tcaacgcctt ctttgccccg gactatgcgg
    13681 gcattatcca gggaatgcgt ttcaacaaag tggatatcgc ctggtacggc aacctgtcgg
    13741 cgatggaagc agtggatcgc gccaacggcc aggtcttcgc tcagaccgtc gcggcggatg
    13801 gatcgccggg ttactggagc gtgttgatcg tcaacaaaga cagcccgatc aacaacctga
    13861 gcgatctgct ggcgaagcgg aaagatctca ccttcggcaa tggcgatcct aactccacct
    13921 ctggcttcct cgtccccggt tactacgtct ttgccaaaaa caatatctcc gctaacgact
    13981 ttaagcgcac cgtcaacgcc gggcatgaaa ccaacgcgct ggccgtcgcc aacaagcagg
    14041 tggatgttgc cactaacaac accgaaaacc tcgacaagct gaaaacctcc gcgccggaga
    14101 agctcaaaga gctgaaagtg atctggaagt cgccgctgat cccaggcgat ccgatcgtct
    14161 ggcgcaagaa tctgtccgaa accaccaaag acaagatcta cgacttcttt atgaattacg
    14221 gcaagacgcc ggaagagaaa gcggtgctgg aacgcctggg ctgggcgccg ttccgcgcct
    14281 ccagcgacct gcaactggtg ccgattcgcc agctcgcgct gtttaaagag atgcagggcg
    14341 tgaaaagcaa taaaggactg aatgagcagg acaagctggc aaaaaccacc gagattcagg
    14401 cgcagctgga tgacctggac cgcctgaaca acgcgttaag cgcgatgagt tcggtgagta
    14461 aagcggtgca gtaaatcgta ggtcggataa gacgccccgg cgtcgcatcc gacaatgtgc
    14521 aggcgttgat gccggatgcg gtgcaagcac cttatccggc ctacagaccg gagccaaaca
    14581 tgcaaaccat caccatcgcc ccacccaagc gtagctggtt ctcgcttctg agctgggccg
    14641 ttgttctcgc cgtgctggtc gtctcgtggc agggcgcgga aatggccccg cttacgctta
    14701 tcaaagacgg cggcaacatg gcaaccttcg ccgccgactt cttcccgccc gatttcagcc
    14761 agtggcagga ttacctcacc gaaatggccg tcacgctgca aatcgccgta tggggcaccg
    14821 cgctggcagt ggttctgtcc atcccgttcg gtctgatgag cgccgaaaac ctggtgccgt
    14881 ggtgggttta ccagcccgtg cgccgcctga tggacgcctg ccgcgccatt aacgaaatgg
    14941 tcttcgccat gctgttcgtg gtcgccgtcg gtctcggacc gttcgctggc gtgctggcgc
    15001 tatttattca caccaccggc gtgctctcca agctgctttc cgaagcggtg gaagcgattg
    15061 aacctggccc ggtggaaggc attcgcgcca ccggtgccaa caaactcgaa gagatcctct
    15121 acggcgtgct gccacaggtg atgccgctgc tgatctccta ctccctctat cgcttcgaat
    15181 ccaacgtccg ctcggcgacc gtcgtcggca tggtcggcgc gggcgggatc ggcgtcacgc
    15241 tgtgggaagc gattcgcggt ttccagttcc agcaaacctg cgccctgatg gtgctgatta
    15301 tcgtcacggt cagcctgctg gatttcctct ctcaacggtt gcgtaagcac tttatctgat
    15361 tagcgaggca ttgatagcta tgcacttgtc tacacatccg accagctacc cgacacgcta
    15421 tcaggagata gccgcaaaac ttgagcagga gcttcgtcaa cactaccgct gcggcgacta
    15481 tcttcccgcc gagcagcaac tggcagcgcg ctttgaggtg aaccgccaca ccctgcgccg
    15541 cgccatcgac caactggtgg aaaaaggctg ggtacagcgc cgtcagggcg tcggcgtact
    15601 ggtgctgatg cgcccgttcg attacccgct caacgcccag gcgcgtttta gccagaacct
    15661 gctggatcag gggagccatc ccaccagcga aaaactgctg tctgtgctgc gcccggcctc
    15721 cggtcacgtt gcagacgcac tggggattac cgagggcgag aacgttatcc acctgcgcac
    15781 cctgcgccgg gtcaacggcg ttgcgctctg tttaatcgac cactacttcg cggacctcgc
    15841 cctctggccg acgctgcaac gcttcgacag cggctcgctg cacgattttc tgcgcgagca
    15901 aacggggatt gcgctgcgcc gcagccagac gcggatcagc gcccgccgcg cccaggccaa
    15961 agagtgccag cgtcttgaaa tcccgaatat gtcgccgctg ctgtgcgtgc gcacccttaa
    16021 ccaccgtgac ggtgaaagca gcccggcgga gtactccgtc agcctgacgc gcgccgacat
    16081 gattgaattt actatggagc actgaatgca cgcagatacc gcgacccgcc agcactggat
    16141 gtccgtactg gcgcacagcc aaccggcaga actggcagca cgcctgaacg cgctaaacat
    16201 caccgccgac tatgaggtga tccgcgccgc tgaaactggc ctggtacaga ttcaggcgcg
    16261 gatgggcggc accggcgaac gcttttttgc cggcgacgcc acgctgaccc gcgccgccgt
    16321 acgtctgact gacggcaccc tcggctacag ctgggtgctg gggcgcgaca aacagcacgc
    16381 cgaacgctgc gcgctgattg acgcgctgat gcagcaaacc cgccattttc aaaacttatc
    16441 agaaacgctt attgccccgc tggacgctga ccgtatggca cgcattgccg cacgccaggc
    16501 cgaagtgaac gccagccggg tcgacttctt tacgatggtt cgcggagaca acgcatgacc
    16561 ctggaaaccg cttttatgct tcccgtgcag gatgcccagc acagttttcg tcgcctgtta
    16621 aaggccatga gcgagccggg cgtgattgtc gccctgcatc agctcaaacg cggctggcag
    16681 ccgctgaata tcgccaccac cagcgtgctg ctgacgctgg ccgataacga cacgccggtg
    16741 tggcttgccg cgccattgag caacgatatc gtcagccaga gcctgcgttt tcataccaac
    16801 gcgccgctgg tcagccagcc ggaacaggcg accttcgcgg taacggatga ggcgatttcc
    16861 agcgaacagc ttaacgccct ttccaccggc accgccgttg cgccggaagc gggcgcaacg
    16921 ctgattttac aggtcgccag cctgagcggc gggcgcatgc tgcgtctcac cggcgcgggt
    16981 attgccgaag aacgaatgat cgccccgcag ctgccggagt gcattctgca cgaactcacc
    17041 gagcgcccgc acccgttccc gctcggcatc gacctgatcc tgacctgcgg cgagcgcctg
    17101 ctggctgttc cgcgaaccac tcatgtggag gtgtgctgat gtacgttgcc gtgaaagggg
    17161 gcgagaaggc gatcgacgcc gcccacgccc tgcaagagag ccgacgccgg ggcgataccg
    17221 atttgcctga actgagcgtc gcccagattg aacagcagct taacctcgca gtagatcgcg
    17281 tgatgaccga aggcggcatt gccgaccgcg aactggcggc gctggcgctg aaacaggcca
    17341 gcggcgataa cgtcgaagca attttcctgc tgcgcgccta ccgcaccacg ctggcgaagc
    17401 ttgcggtaag cgagccgctc gacaccacgg agatgcgtct cgaacggcgt atttcagcgg
    17461 tttataaaga cattcccggc ggccagctgc ttggcccaac ctacgactac acccatcgcc
    17521 tgctcgattt caccctgctg gcaaacggcg aagcgccgac gctgaccacc gccgacagcg
    17581 aacaacagcc gtcgccgcac gttttcagcc tgctggcgcg tcaggggctg gcgaagtttg
    17641 aagaggatag cggcgcacag ccggatgaca tcacccgcac gccaccggtt tacccctgct
    17701 cacgttcttc ccgcctgcaa cagctcatgc gcggcgacga aggctatttg ctggcgctgg
    17761 cctactccac ccaacgcggt tacgggcgca atcacccgtt cgcgggtgaa atccgcagtg
    17821 gttacatcga cgtgtcgatt gtgccggaag agctgggatt tgcggtgaac gtcggcgaac
    17881 tgctgatgac tgagtgtgaa atggtcaacg gttttatcga cccaccggat gaatctccgc
    17941 acttcacgcg cggctacggg ctggtgttcg gcatgagcga gcgcaaagcg atggcgatgg
    18001 cgctggtcga ccgcgctctg caagccccgg agtacggcga gcacgcgaca ggcccggcgc
    18061 aggatgaaga gttcgtgctg gcacatgccg acaacgtcga agccgcaggc tttgtctcgc
    18121 acctcaaact cccgcactac gtcgatttcc aggccgaact ggagctactc aaacgtctgc
    18181 aacaggagca gaaccatggc taatctgagc ggctacaact ttgcctacct cgacgagcag
    18241 accaaacgca tgatccgccg cgccatctta aaagcggtgg caatccccgg ttatcaggtg
    18301 ccgtttggcg ggcgcgagat gccaatgccg tacggctggg gaaccggcgg cattcagctt
    18361 accgccagcg tgattggcga aagcgacgtg ctgaaggtga ttgaccaggg cgcggatgac
    18421 accaccaacg ccgtgtcgat tcgcaacttc tttaagcgcg tgaccggggt aaacaccacc
    18481 gagcgcacgg acgatgcaac ggtcatccag acgcgtcacc gcatccccga aacgccgctg
    18541 accgaagatc agatcattat tttccaggtg ccgatccccg aaccgctgcg ctttatcgag
    18601 ccgcgcgaaa cggaaacccg caccatgcac gcgctggagg agtacggcgt gatgcaggtg
    18661 aagctgtatg aagatatcgc ccgcttcggt catatcgcca ccacctacgc ctatccggtg
    18721 aaggtgaacg ggcgctacgt aatggacccg tcgccgatcc cgaaattcga taacccgaaa
    18781 atggacatga tgcccgccct gcaactgttc ggcgccgggc gtgagaagcg catctatgcg
    18841 gtgccgccgt ttacccgcgt ggaaagcctc gatttcgacg atcacccgtt caccgttcag
    18901 cagtgggatg agccatgcgc catctgcgga tcgacccaca gctatcttga tgaagtggtg
    18961 ctggatgacg ccggaaaccg catgtttgtc tgctccgata ccgattattg ccgccaacag
    19021 agcgaggcaa aaagccaatg aatcaaccgt tactttcggt caataacctg acccaccttt
    19081 acgcgccggg caaaggcttt agcgatgtct cttttgattt atggccgggg gaagtgctgg
    19141 gcattgtcgg ggaatccggc tccgggaaga ccacgctgct gaaatcaatt tccgcgcgtc
    19201 tgacgccgca gcagggggag attcactatg aaaaccgttc cctctatggc atgagcgagg
    19261 ccgaccgccg tcgcctgctg cgcaccgaat ggggcgtggt gcatcagcat ccgctcgacg
    19321 gcctgcgccg ccaggtgtcg gcaggcggca atatcggcga gcggctgatg gcgaccgggg
    19381 cacgtcatta cggcgatatt cgcgccaccg cgcagaagtg gctggaagag gtggaaattc
    19441 ccgccaaccg gatcgacgac ctgccgacca ccttttccgg cggcatgcag cagcgtttgc
    19501 agattgcccg caacctggtg acgcatccga agctggtatt tatggacgag ccaaccggcg
    19561 ggctggatgt gtcggtgcag gcgcgcctgc tcgacctgct gcgcggcctg gtggtggagc
    19621 tgaacctcgc ggtggtgatt gtcacccacg atttaggcgt cgcccgcctg ctggcggacc
    19681 gtttgctggt gatgaagcag gggcaagtgg tggagagtgg gttaaccgac cgcgtgctcg
    19741 acgacccgca tcatccgtat acacagctgc tggtgtcatc ggttttgcag aattgagccg
    19801 gtgccggatg cggcgtaaac gccttatccg gcctacaaat gcgctccccg taggtcggat
    19861 aagacgcgtc agcgtcgcat ccgacacccg aaccacgagg cgaaaaatga ttaacgtaca
    19921 aaacgtcagt aaaaccttca tcctgcatca gcaaaacggc gtgcgcctgc ccgtcttaca
    19981 acgcgcctca ctcaccgtca acgcgggcga atgcgtggtg ctccacggcc actccggcag
    20041 cggcaaatca accctgctgc gctcgctgta cgccaactat ctgcccgacg aagggcaaat
    20101 ccagatcaaa cacggcgacg aatgggtcga tctggtcacc gcgccagcgc gcaaagtggt
    20161 ggagatccgc aaaaccaccg tcggctgggt gagccagttt ctgcgcgtca tcccgcgtat
    20221 ttcagcactg gaagtggtga tgcagccgct gctcgatacc ggcgtaccgc gtgaagcctg
    20281 cgccgctaaa gccgcgcgtc tgctcacccg cctgaacgtg ccggaacgcc tgtggcacct
    20341 ggcgccatcg accttctccg gcggcgaaca gcagcgcgtc aacatcgccc gcggctttat
    20401 cgtcgactac cccattctgc tgctcgacga acctaccgcc tcgctggacg ctaaaaacag
    20461 cgccgccgta gtggaactga tccgcgaagc caaagcccgt ggcgcagcca tcgtcggcat
    20521 tttccatgac gaagccgtac gcaatgatgt cgccgaccgc ctgcacccaa tgggagcctc
    20581 ttcatgatta tcaataacgt taaactggtg ctggaaaacg aagtggtgca cggttcgctg
    20641 gagatgcagg atggtgaaat ccgcgccttt gccgaaagcc agagccgcct gccggaggcg
    20701 atggacggtg aaggcggctg gctgctgccg ggattgattg agctgcatac cgataacctc
    20761 gataaattct tcaccccgcg cccgaaagtc gactggcccg cccattctgc gatgagcagc
    20821 cacgacgcat taatggtggc aagcggcatc accaccgtac tggatgcggt ggcgattggc
    20881 gacgtgcgcg acggcggcga tcggctggag aatctggaga agatgatcaa cgccatcgaa
    20941 gagacgcaga aacgcggcgt caaccgcgcc gagcaccgcc tgcacctgcg ctgcgagctg
    21001 ccgcatcaca ccacgctgcc gctgtttgaa aaactggtgc agcgcgagcc ggtgacgctg
    21061 gtgtcgctga tggaccactc gccgggccag cgccagttcg ccaaccgcga gaagtaccgt
    21121 gaatattatc agggcaaata ctcgctcact gatgcgcaga tgcagcagta cgaagaagag
    21181 caactggcgc tcgccgcacg ctggtcgcag ccgaatcgcg aatccatcgc cgccctgtgc
    21241 cgcgcgcgaa aaattgcgct tgccagccac gatgacgcca cccacgccca cgttgccgaa
    21301 tcccatcagc ttggcagcgt gatcgccgaa tttcccacca cgttcgaagc ggcagaagcc
    21361 tcgcgcaagc atggtatgaa cgtgctgatg ggcgcgccaa atatcgtgcg tggcggttcg
    21421 cactccggca acgtggcggc cagtgaactg gcgcagcttg gcctgctgga tatcctctct
    21481 tccgactact accccgccag cctgctggat gcggcgttcc gcgtggccga tgaccagagc
    21541 aaccgcttta cgctgccgca ggcggtgaag ctggtgacta aaaatccggc gcaggcgctt
    21601 aatctccagg atcgcggggt gattggcgag ggcaaacgcg ccgacctggt gctggcgcat
    21661 cgcaagggca accacattca tatcgaccac gtctggcgtc agggtaaaag ggtgttctga
    21721 tgacgggaaa actgatttgg ttaatggggc cgtccggctc cgggaaagac agcctgctgg
    21781 cggaactccg cctacgggaa caaactcagt tactggtggc gcatcgctac atcacgcgcg
    21841 acgccagcgc tggaagcgaa aaccatatcg ccctgagcga gcaggagttt tttacccgcg
    21901 cggggcaaaa cctactggcc ttaagctggc acgccaacgg cctgtattat ggcgtcggcg
    21961 tcgagataga cctctggctg cacgccggat tcgacgtgct ggtcaacggc tcacgcgccc
    22021 atctgccgca ggcgcgggca cgctatgcat cggcactgct gcccatctgc ttacaggtgt
    22081 cgccagagat cctgcgccag cgcctggaaa accgtggtcg tgaaaacgcc agtgaaatta
    22141 acgcccggct ggcgcgcgcc gcccgctata ctcctcagga ttgccatacg ctcaacaatg
    22201 acggcagcct gcgccagtcg gtcgacacgc tgctgacgct gattcatcag aaggagaaac
    22261 accatgcctg cttgtgagct tcgcccggcc acgcagtacg acaccgacgc ggtttacgcg
    22321 ctgatttgtg agctaaaaca ggcggagttt gaccatcacg cgtttcgcgt gggctttaac
    22381 gctaacctgc gtgacccaaa catgcgctac catctggcgc tgctggatgg cgaagttgtc
    22441 ggtatgatcg gcctgcattt gcagtttcat ctgcatcatg tcaattggat cggcgaaatt
    22501 caggaactgg tggtgatgcc gcaggcgcgc ggtctgaacg ttggtagcaa gttgctggcg
    22561 tgggcagaag aagaagcccg ccaggccggg gccgaaatga ccgagctttc gaccaacgtg
    22621 aagcgccacg acgcacaccg tttctatctg cgcgaaggct acgagcagag ccacttccgc
    22681 ttcacgaagg cgctgtaaca tgagcctgac cctcacgctc accggcaccg gcggcgcaca
    22741 gggcgttccg gcatggggct gcgagtgtgc ggcctgcgcc agagcgcggc gctcaccgca
    22801 ctatcgccgc cagccatgca gcggcgtagt gaagtttaac gaggcgatca ccctgatcga
    22861 cgccgggcgg cacgatctcg ccgatcgctg gtcgccagga tcgttccagc agtttttgct
    22921 gacgcactac catatggatc acgtccaggg gctgtttccg ctgcgctggg gcgtgggcga
    22981 tacgatcccg gtttacggcc cgccggatga ccagggttgc gacgatctgt ttaaacatcc
    23041 gggcctgctt gatttcagcc acacagtgga accgtttgtg gtgtttgatt tgcaggggtt
    23101 gcaggtcacg cccctgccgc tcaaccactc aaaactgacc ttcggttatc tgctggaaac
    23161 ggcgcacagc cgggtggcgt ggctgtctga caccgctggc ttgccggaaa aaacgctgaa
    23221 atttttacgc aataatcagc cgcaggtaat ggtgattgat tgcagccacc cgccgcgcga
    23281 ggatgcgcca cgtaatcact gtgatttaaa caccgtgctg gcgctgaatg aagttatccg
    23341 ctcgccgcgg gtgattctga cacacatcag ccaccagttt gatgcgtggc tgatggagaa
    23401 cgcgctgccg ccggggtttg aagccgggtt tgatgggatg gagattgggg tggcgtgatg
    23461 agagggaatg tgcgcgctgg ccccctcacc ctaaccctct ccccagaggg gcgatgcctg
    23521 ctccataccc aacatcatcg cccatactca tcttccattc tccgctcttc atcctccagc
    23581 tgccgacgct cctgatcaag ctggcgctgg cgatcgtcca gctgcctgcg gcgatcttca
    23641 aactggcggc ggcggtcgtc atattgtctg cgccgatcgt cgctcacttc acgctgccag
    23701 ccatcgtcgc gcgaatcttc ataatctcgc ccacggtcag ggttataagc gtcgttaatc
    23761 gcctgctgaa tattgccaat ggtgtcgtcg ataatatcgg cctgggccgg tacggtggtc
    23821 agcgtgagca gggtgaaaaa aaagagcgtg gagtggcgtt tcataaactc ggcctcgcaa
    23881 caaagttaat acaaggtagg caaaggtgcc tgttacgagt agaggaggat tctcaattct
    23941 cttgccccag cgcctgcgcc aactgcgcac gggtaaaggg tttgcgcaac agcgccacgt
    24001 ctggcagcgc ggggttatgg ctggggcgta aatcctgacc gctgatgagt agcagcgtca
    24061 ggtgcgggta aagtttgcgg gccgcattga ccacttctgc gccgctcatg ccgccgggga
    24121 gcattaaatc gctgataaaa atatcgattt ctgctgatgc cgccagcaga tccagcgcct
    24181 gttcgccgct gcttgcttcc agcgtcaaat agcccagcag gtgcaactgt tcgcagatgg
    24241 tctggcgcac cgctgcttca tcttccagca ctaacaccag tttatcgccg ctgacagaag
    24301 cctgttctac tgccgctagc gataggtttt tcgccgccgt cagcgcgcgg ggaagctgta
    24361 atctgaccgt tgtcccctgc cccggcgcgc tttcaatttc aacgcgccca ccggactggc
    24421 gcacaaaacc atagaccatc gacaaaccca gcccgctgcc gctgcccgtc tgtttggtgg
    24481 taaagaatgg ctcaaatacc tggcttttca ccgcctgcga cataccgctg ccatgatcga
    24541 tgacctccag tgccaccatg tcctgacgct gcccactgct gcgagtaacg cgctgattcc
    24601 aggtacgaat tttgatgatt cccgtctgcc cttccatcgc gtcacgggcg ttcatcacca
    24661 ggttgatgat ggcattttcc agttggctga tgtcaatcca cgcgggccac gccggagact
    24721 gcgcttcaat ctccagcgcc agcgttgccg gtaaggagtg gcgcattaat tcgcttaact
    24781 cttccagcaa tgttttcagt tcaacggctt gcggatgtaa ggactgcttg cgggaaaacg
    24841 ccagcagtcg ttgtgtcagt agcgccccac gttcggcagc ttgcagcgcc cgctgtattc
    24901 gcggcgcgtc tggcgagtcc ggattcacca gctccaggct gccaataatc accgccagca
    24961 gattattaaa atcatgggcc agcccgcccg tcagttgccc gaccgctttc agtttctggc
    25021 tgtgtaatag cgcctcttcc aacccctgcc gctcagtttg ggcaatctcc atctgcgtgg
    25081 tcttctcttt tagcagccgt gaggttcgtt cgagtgactc ggtattgcgg gcaaaaacgt
    25141 taaaggcgcg cgccagttca cccaactcat cacgccgctg taacgccgga aaagtgacgt
    25201 ccgcttcgcc gtacgccagg cggctcatgg cgcgggaaat cgccgtcagg ttagtgccga
    25261 ggttgcgata gatataccag ccggcaaacg cggtgatcac caccgctaac agggcgaaaa
    25321 tcatgatcac cctctcgccg gagcgcaaat cctggtaact ttgcgccacg cgctgaccgg
    25381 agagcgtcgc cacctgttcg gcgtaacggt tgatgtcgcc attgaggatc gccaccagcg
    25441 ctttgatgtg aaacatatac cagctgattg ccagatcgct ttgctccaga gcggcagaaa
    25501 gcggcgcgag tttacgcatt tcatcattga aatcattgag taacgtatta accaatggca
    25561 tatcgctgtg ggtcggcaac gcagacatta ccccgaccag ttgatgaata atcgcctgcg
    25621 gcgcgggggt ggcaatagcg gcaacaatca gtcggttcat ctcgttaagc aaaatatcat
    25681 cttgcgcgtg agtgactttt tgcagatgcc gcagataact taagttttga tacagcgaac
    25741 ttaacagcgc gttgcgctcc agatgccgcc tttgcccgcg ctccagcata ccgccgacgc
    25801 tggtttgcag ttcgttgcta cgcgtgatga ttcgcgtcac cagcccggct tcctgctgcg
    25861 ctaaaggcgc gttcgccagt tgtgcaagcg agctttttaa tgcccgctgt gtctcctgca
    25921 accgctcggc ttcgccttta tattccagcg ctcccaccac ctgcgacaat cgcaccgccg
    25981 ccgttgccac gtttgccgtg tcgcgcgcca gattcaggct gccggtcata tcatccaccg
    26041 tttgctgctg caccagctcc tgcatctggc tggcatgacg aaagcccatt accgccacgc
    26101 cgctgaccat caacgtcacc gccaccacca gcaaattcaa aagcagcaat cgcccgcgcg
    26161 cgctggcaaa aaaatgacgt ccggggtgcg ccatatcacg ctccgtgaag aaaaatcgca
    26221 atatgacaaa tatgaacggc ttctgacatt ttgcatttac tttcctgtga tggagtgctg
    26281 atatcgacaa cctcgcagga gtccaccatg aacagcacgc cagttctgga gatgcgcaat
    26341 attgccaaag ccttcggcaa attttatgcg ctcaaagggg tggatttgac ggtctaccct
    26401 ggcgagatcc atgccctgat gggggaaaac ggcgcgggaa aaagcacgct gatgaaggtg
    26461 ctggcggggg cgtataccgc caccagcggc gagatcctca tagacggcaa gccctttcac
    26521 attcgcacgc caaaagatgc cttaagcgcc ggtattacgc tgatttatca ggagatgcag
    26581 ctggcaccga atctgtcggt ggcagaaaat atttttctcg gcagcgagct ttcccacggc
    26641 gggctggtgc agcgtaaaga gatgctagtg caggcgcaaa aagtgatcga ccgcctcggc
    26701 gcgcagttta acgccagcga taaggtcatg acgctgacca ttgccgagca acagcaggtc
    26761 gaaatcgccc gcgcactaca tcgcaacagc cgcattctgg tgatggacga acccaccgct
    26821 gccctctcct cccgtgaaac tcaccgtctg tttgaactga ttatgcggct gcgcgatgaa
    26881 gggatggcga ttatctacat cagccaccgt atggcggaag tgtatgagct ttccgatcgc
    26941 gtcagcgtgc tgcgcgacgg gcaatacgtt ggcagcctga cccgcgataa cctcaatgcc
    27001 ggggagctgg tgcggatgat ggtcggcagg ccactgagcg atctgttcaa taaagagcgc
    27061 gatatcccgc tcggtaaagc ccgcctgaat gttcacctcc tgaccgacgg cggcaaagtc
    27121 cagccgagta gcctgctggt gcgttccggc gaaattgttg gcctcgccgg actggtgggt
    27181 gccggacgtt ccgaactggc gcagttgatc ttcggcgtgc ggaaagcgac aggcggaatg
    27241 attgaagtcg atggtgaacc ggtggtgatc cactccccgc gcgaagccat cgatcttgcc
    27301 attggttttc tcaccgaaaa ccgcaaagaa caaggcttat tccttgaaat ggcagcagcc
    27361 gaaaacatca ccatggcaac cctggagcgc gatgcccgct ggggaatgct caatcgcaaa
    27421 aaagcgcaaa ccatttccga tgacgccatt aagttgctca acattcgcgt gcctcatgcc
    27481 caggtacgcg cgggcgggct ttccggcggc aatcagcaaa aactgttgat ctcccgctgg
    27541 gtggcgattg gcccgcgcat tttactgctc gatgaaccca cccgcggcgt ggacgttggc
    27601 gccaaaagtg agatctaccg gatcatgaac gagatggcgc gcaagggcgt ggcgatcctg
    27661 atgatctcca gcgaactgcc ggagatagtg ggaatgagcg atcgcgtcta tgtgatgcgc
    27721 gaaggcagca ttgccggtga gttaaacggc aaaaacatca cccaggaaaa cattatgacc
    27781 ttagcgactg gcgtcaacga cgcccattcc caggcggtaa cctcatgacg acctctgaaa
    27841 aaacctctgc caaaaaaata ctgatgagcg atctgatgca aacggtcggc atcttgccca
    27901 ttttgattct gatcgtggcg gtatttggct ttatcgcgcc aaactttttt accgaaagca
    27961 acctgctgaa tattacccgc caggcatcga tcaacattgt tctggcggcg gggatgacct
    28021 tcatcatttt gaccggcggg atcgatcttt ccgtgggttc gatcctcggc accacagcgg
    28081 tggcggcgat ggtcgtctcc cttagccctg agttcgccat gctgtcgatc ccggcagcgc
    28141 tgatgctcgg cctgctgctg gggctgttca acggcgcgct ggtggccttt gccggattgc
    28201 caccgtttat cgttacgctt ggcacctata cggcgctgcg cggggcggcg tatctgctgg
    28261 cggacggcac cacggttatc aactccaaca ttaatttcga gtggataggc aacaactatc
    28321 tcgggccggt cccatggctg gtggtgattg cgctggcggt gatcgtggtg tgctggttta
    28381 ttctgcgccg caccacgctt ggcgttcata tctacgccgt cggcggcaat atgcaggcgg
    28441 cgcgtcttac cggcattaag gtctggctgg tgctgctgtt tgtttatggt atgagcggcc
    28501 tgctttcagg gctgggcgga attatgagcg cctcaaggct gtacagcgcc aacggcaact
    28561 taggtatggg gtacgagctg gacgccattg cggcggtgat cctcggcggc accagttttg
    28621 tcggcggcat cggcaccatc accggcacgc tggtcggcgc gctaattatc gccacgctaa
    28681 acaacggcat gaccttaatg ggcgtctctt acttctggca actggtgatc aaaggggcgg
    28741 tgatcatcat tgcggtgctg atcgacaaat accgtacccg acaccatcaa agtgcataaa
    28801 cacatgagga atacagcatg agattgaagc ccttagtgac cgcgctctgt gcaggcgcgc
    28861 tgctggccgc agcaccgttc gcccaagcga aagaactgaa atcgataggc gtaacggtgg
    28921 gcgatctggc gaacccgttc ttcgtgcaga tcaccaaagg cgcggagctg gaagcacgca
    28981 aactggcagg cgataacgtc aaagtgacgc tggtttccag cggctacgat ctgggtcagc
    29041 aggtgtcgca gatagacaac tttatcgccg ctaacgtcga tatgattatc ctcaacgctg
    29101 ccgattcaaa agggatcggc ccggcggtga aacgggcgaa agatgccggg atcgtagttg
    29161 tcgcggttga cgtagcggca gaaggtgctg atgccaccat cacctccgat aacacccagg
    29221 cgggcgaaat ggcctgtaaa tacatcaccg atcgcctgaa aggcaaaggc aatgtggtga
    29281 tcatcaacgg cccgccggta tccgccgtgc aaaaccgcgt cgaaggatgc cagactgaat
    29341 ttaaacgcca tcctgacatc aaagtgctct ctgacaacca gaacgcgaaa ggcagtcgtg
    29401 aaggcgggct ggaggtaatg acgggactgc tggcggcaaa tccgaagatc gacggcgtat
    29461 ttgcgattaa cgatcccacc gcaatcggtg ccgatctggc agcaaaacag gcgcaacgta
    29521 acgagttctt tattgtcggt gtggacggaa gcccggacgc cgaagaggcg ctcaagcgtg
    29581 agaattcgtt gtttgtcgct actccggcgc aagatcccca ggtgatggca gcgaaagcgg
    29641 tggaaattgg ctacgacatt ttgcagggca aacctgcgcc gaaagagccg gtgctgatcc
    29701 cggtgacgat gatcgataaa aacaacgtcg gcaattacaa aggctggacg gtgaaataag
    29761 tccacacgcc gggcggcgca gctgcccggt caccgctaag gagaacgctt tcgatgaaac
    29821 gctccgctat aaacgacatt ctcggccata cacggcaatt cttttcgcaa catgacgtac
    29881 atctgccgcc ctttgccagt ttctcgcctg ctcaatggca gcagctcgac accgccgcgt
    29941 gggaggaggt ttttgatctt aaactcggct gggatgttac cgcttttggt cgcaacaact
    30001 ttgcagcaca cgggctgacg ctgtttaccc tacgtaacgg ctcagcaaaa ggaatgccgt
    30061 atgtgaaatg ttacgccgag aagatcatgc atgtgcgcga tgcgcaggtc acgccaatgc
    30121 attttcactg gcgtaagcgt gaggacatta tcaatcgcgg cggcggtaat ttaattgttg
    30181 aactgtggaa tgccgatagt aacgagcaaa cggctgatag tgacattacg gtggtgatag
    30241 acggctgccg ccagaaacat actgcaggca ctcagttgcg cctgtctccc ggcgaaagta
    30301 tctgcctgcc gcccggcctg tatcacagct tttgggcgga aaccggcttt ggcgatgttc
    30361 tggttggcga agtctcttct gtcaatgacg acgaccacga taaccacttt ttacagccac
    30421 tggaccgcta caacctgatt gacgaagacg aacctgcaca gttggtattg tgtaacgaat
    30481 accgccagtt ccgctgaata aggataacgt tatgccattg atttctctcg ctgaagggct
    30541 ggcacatgcc cgcgaacatc actattcgtt aggtgcgttt aatgtgctgg actcgcactt
    30601 tttacgcgcc ttgtttgctg ctgccagaca agaacgttcg ccctttatta tcaacattgc
    30661 cgaagtacat tttaaatata tctcgctgga ttccctggtt gagacagtga aatttgaagc
    30721 ggcgcgtcat gccattccgg tggtgttaaa tctcgatcac ggactgcatt ttgaatcggt
    30781 ggtgcaagcg ttgcggctgg gattcagctc ggtaatgttt gatggctcca cgctggggta
    30841 tgaagaaaat gtccgccaga cgcgggaagt ggtgaaaatg tgccatgccg ttggcgtatc
    30901 ggtagaagct gaactgggcg cggtgggtgg cgacgaaggc ggtgcgctct atggtcatgc
    30961 cgatgaatcc ctgtttaccg atccgcaact ggcgcgtgat tttgtcgacc gtaccggcat
    31021 tgacgcgttg gccgtggcga ttggcaatgc acacggcaaa tatcgcggcg aaccgaaact
    31081 cgatttcccg cgcctcgacg ccatccgcca gcaagcggct atcccgctgg tgttacacgg
    31141 cggctcaggg attagcgatg ccgatttccg ccgcgccatc gaactgggta tccacaaaat
    31201 caatttctac actggcatgt cgcaagcagc gctggctgcc gttgaggccc gaatggcgca
    31261 ccgtcagccg atgtatgacg agtttgccga gttactgctg gcagtggaag ccgccatcag
    31321 cgatacggtt gcacaacaga tgcgtatttt ctgtagtgcg gggcaggcat gatggagcgt
    31381 aaagggatta tcgccgcagg caatatgctg gttgaccacg tccatcagat tatccagtgg
    31441 ccagagcgcg gctggctggc ggagatcacc cacagcgaac gctccaccgg cggtgcgccg
    31501 cttaacgtcc tgctgacgct ggcgaaaatg cgcaccgggc tgccgttaca ggcggttggg
    31561 ctaatcggtc aggacagcga cggcgattac atcatggcga tgcttgagca atatcacgtc
    31621 aatcgtcagc atgtgcagcg cactaccttt gccccgacgt cgatgtcgca ggtgatgaca
    31681 gacccaagcg ggcagtggac gtttttccat tcgccgggtg caaacagact tttggatctt
    31741 ccggcgttcg atcagctcga taacaccatg aagattttcc atctcggcta tttactgttg
    31801 ctcgacagcc tggatatgcc ggacgatgtt tacggcaccc gtagcgcacg cctgctggcg
    31861 caaatgcgtg atactggctt tgaaacctca ctcgacctgg tctcccgtaa gggcgatccg
    31921 cgttatcagc cgctggtgct gcccgcgttg cgccatctgg attatctggt aattaacgaa
    31981 ctggaggccg gggagtttag cgggctggaa attcggttac ccaacggcga accgcatatc
    32041 gcgcatattg ctgccgccgc gcgtgaactg ttagatgcgg gcgtgcgcca gcgggtggtg
    32101 atccactgcc cggaaggggc gtggggagag accccagaac agggcggcgt gtggatccct
    32161 tcgcaaaagt tagaacaacg ggaaattatc ggcagcgtcg gcgcgggcga tgcattttgt
    32221 gccggatttt tatatggctg ccatgaacgc tggacgctta ccgagagcat caaactggca
    32281 cacgcctgcg cgcgggcaag cctgttatgc gccaatgcca tcgacggggc gaaaacgctg
    32341 gaagagctgc aaacagaaat gtcggattaa tgcacgtcgg cagcgaagac ataccccaga
    32401 ccgcgcagag ttttaatcaa catcggctga tgcgggttga gttcgatttt gcgtcgtaat
    32461 cgcatgatta atacgtcgat ggtgcgatca aacacctcca cgctgtcgct gtgggtgaac
    32521 gccagcaact gctcgcggct caatacccga cgcgcgtttt gcgccagagc cagtaacaag
    32581 ccatattcgc cctgagttaa ctcgacatgt tgctgaagcg gattgtgcaa ctggcagcgc
    32641 atggtatcga gataccagcc attgaatgac agcccggcgc ttttctcgtc attaaccgcc
    32701 agcgcaccaa cccgtcgcaa caccgctttc agtcgtgcga ccaccacccg cggggtgaac
    32761 ggtttgcaga tgtaatcatc cgcgcccatt tccagcccga ccaccacatc agactcactg
    32821 cccatgccgg tcaacatcac caccggcaat gtcggacgca gtttctggat ttgctgcaat
    32881 accctcaggc cgttggtatc gggtagcatc atatccagca ccaccagcgc aatgtcagga
    32941 tcgccctcaa tacgcaatat ggcctcctgt ccggtatggc agactgaaac cgtaaagaca
    33001 tgttcactta acacgtcctg caacagtgcg cagatagccg tgtcatcatc caccaccagc
    33061 acgacgggtt tcatggttac actccgcatc attcatctga ttaatcattg tgcgtacttc
    33121 ttgtcgaagt gaaagctgaa agtaatataa ataataaaaa tataagtcag gtcacattat
    33181 tgatgatagt aataaatatg ccgtactttt ccatcttcag cacaaatgcc taataaaatg
    33241 gttcgttccg ctcacacttc cacttattaa agtcgcaatt tcccaattat gataacgcca
    33301 gctttaaatg acgtattttg catggattac atatttacga cataaatgat aaatggagat
    33361 ttatatgaat aactctcggt tattccgttt gagcaggatt gttattgcgt taactgccgc
    33421 cagcggcatg atggtaaata ccgctaacgc gaaagaggaa gcgaaagccg ccactcaata
    33481 tacccaacag gttaatcaga attacgccaa atcattaccg tttagcgatc gtcaggattt
    33541 tgacgatgcc cagcgtggat ttatcgcccc gctgctggat gaaggtattc tgcgtgatgc
    33601 gaacggtaaa gtttactacc gcgcggacga ttacaaattt gatattaatg ccgcagcgcc
    33661 ggaaaccgta aaccccagcc tgtggcgtca gtcgcaaatc aacggtattt ctggcctgtt
    33721 caaagtcacc gataaaatgt atcaggtgcg cggccaggat atctctaaca ttacgttcgt
    33781 tgagggcgag aaaggcatta ttgttatcga cccgctggtg acgccgcctg ccgcaaaagc
    33841 cgcacttgac ctttacttcc agcatcgtcc gcaaaaaccg attgttgccg ttatctacac
    33901 tcacagccac accgaccact atggtggcgt gaaaggcatt atctctgaag ccgatgttaa
    33961 atccggcaaa gttcaggtga ttgcccctgc tggctttatg gacgaagcca tcagcgaaaa
    34021 cgtgctggcg ggtaacatca tgagccgccg tgcgctctac tcttacggtc tgttactgcc
    34081 gcacaacgcg caaggcaacg tgggtaatgg ccttggcgtg acgctggcaa cgggcgaccc
    34141 gagcattatt gcaccgacta aaactatcgt ccgaactggc gagaagatga ttatcgacgg
    34201 cctggagttt gacttcctga tgaccccagg tagcgaagca ccagccgaaa tgcacttcta
    34261 tattccggcc ctgaaagccc tgtgtaccgc cgagaacgcc acgcataccc tgcacaactt
    34321 ctacactctg cgcggcgcga aaacccgcga taccagcaag tggaccgagt acctgaacga
    34381 aacgctggat atgtggggta acgacgcgga agtactgttt atgccgcaca catggccggt
    34441 ctggggcaat aagcatatca atgattatat tggtaaatat cgcgatacta tcaagtacat
    34501 tcacgaccag accctgcacc tggcgaacca gggctacacc atgaatgaaa tcggcgacat
    34561 gattaagctg ccgcctgcac ttgccaataa ctgggccagc cgtggctatt acggttctgt
    34621 cagccacaac gcccgcgcgg tgtataactt ctatcttggc tattacgacg gtaacccggc
    34681 taacctgcat ccgtatggtc aggtagagat gggcaaacgt tacgtgaaag cgctgggcgg
    34741 ttctgcccgt gtcatcaacc tggcgcaaga agcgaacaag caaggtgatt accgctggtc
    34801 ggcagaactg ctgaaacagg tgattgccgc caacccgggt gaccaggtcg cgaagaatct
    34861 gcaagcgaat aactttgaac agctgggcta tcaggccgag tccgccacat ggcgcggttt
    34921 ctacctgacc ggcgcgaaag agctgcgcga aggggtgcat aagttcagcc acggcaccac
    34981 cggttccccg gacaccattc gcggaatgtc ggtcgaaatg ctgttcgact ttatgtccgt
    35041 tcgcctcgat agcgaaaaag ccgcgggtaa aaatatcagc ctgaacttca atatgagcaa
    35101 cggcgataac ctcaacctga cgctgaacga tagcgtgctt aactaccgta aaacgctgca
    35161 atcccaagct gacgcctctt tctacatcag tcgtgaagat ctgcacgccg tgctgaccgg
    35221 acaagccaaa atggcggatc tggtaaaagc gaagaaagcc aaaattattg gcaatggtgc
    35281 gaaactggaa gaaattatcg cctgtctgga taatttcgat ttgtgggtga atatcgtaac
    35341 cccaaattaa tgccactcac ttttgggggc gcttaagccc ccaaaagcat ttacttttaa
    35401 aatatatgca gccaattaat aaataaaata tataagttat gaatgcggtg gctgaataaa
    35461 gctattttta ttcatcaaga aaatagccat actgacgcct tcaatggata ttgatattca
    35521 ctacagatat tgttcgcagc agacaggatg tatttaatat gccaacagtt ctctctcgca
    35581 tggcgatgca actcaaaaaa acagcctgga taattcccgt cttcatggtt tcgggatgtt
    35641 cattatctcc ggcaatcccg gtgatcggcg cttattatcc cagctggttt ttctgcgcca
    35701 ttgccagcct tattttgacg ctcatcacga ggcgaattat tcagcggaca aatatcaatc
    35761 tggcatttgt cggaattatt tataccgccc tttttgctct ctacgccatg ctgttctggc
    35821 tggcattttt ctaatctatt tgagacgcgc tcatggaaag tacgccgaaa aaagctcctc
    35881 gcagtaaatt ccctgctctg ttagtggttg cgttggcgct ggttgccctt gttttcgtta
    35941 tctggcgcgt agacagtgcg ccatcaacta atgacgctta cgcgtcagca gataccattg
    36001 atgtggtgcc ggaagtcagc ggccgcattg tagaactggc ggtcaccgac aaccaggcag
    36061 tcaaacaggg cgatttgctg ttccgcattg acccgcgccc gtacgaagcc aatctggcga
    36121 aagctgaagc ctccctcgcg gcactggata agcaaattat gctcacccag cgtagcgttg
    36181 acgcgcaaca gtttggtgcc gactcggtta acgccacggt agaaaaagcc cgtgccgccg
    36241 ctaaacaggc cacagataca ttacgccgca ccgagccatt actgaaagaa ggttttgtct
    36301 cagcggaaga cgttgaccgt gcgaggaccg cgcagcgcgc tgcagaagcg gatcttaatg
    36361 ccgtattgtt acaggcgcag tcagccgcca gcgccgtcag cggcgtcgat gcgctggtgg
    36421 cgcaacgtgc ggcggtcgaa gcggatattg ccctgaccaa actgcatctg gaaatggcga
    36481 ccgttcgcgc accgtttgat ggccgggtca tttccctcaa aacctccgtc gggcaatttg
    36541 cttctgccat gcgccctatt tttaccctaa tcgacactcg tcactggtac gtgatcgcca
    36601 acttccgcga aaccgatctg aaaaatattc gctcaggtac acccgcaacg attcgcctga
    36661 tgagtgacag cggcaaaact ttcgagggta aagtggattc gattggctac ggcgtgctac
    36721 cggatgacgg cggcctggtg ctgggcggcc tgccaaaagt ttctcgctct attaactggg
    36781 tccgcgttgc ccagcgtttt ccggtcaaaa tcatggtcga taaacctgac ccggaaatgt
    36841 tccgcatcgg cgcttcggca gtcgctaatc ttgagccgca ataatgagcg cgctcaactc
    36901 cctgccatta ccggtggtca ggctgctggc attctttcat gaagagttaa gcgagcggcg
    36961 accaggtcgc gtgccgcaga ccgtgcaact ctgggtaggc tgcctgctgg tgattctgat
    37021 ctcgatgact tttgagatcc cttttgtggc gttatcgctg gcagtgctgt tttacggtgt
    37081 tcagtcgaac gcgttttaca ccaaatttgt cgcgatcttg tttgtggttg ccacggtgct
    37141 ggagatcggc agcctgtttt tgatctacaa atggtcatac ggcgaaccgt tgatccgctt
    37201 gatcatcgcc gggccgatcc tgatgggctg tatgtttttg atgcgcaccc atcggttggg
    37261 gctggtcttt ttcgccgtcg ccattgtcgc tatttacggg caaaccttcc ccgccatgct
    37321 cgactatcca gaagtggtcg tgcgcttaac gctgtggtgt atcgttgttg gcctctatcc
    37381 aaccttgctg atgacgttaa tcggcgtact gtggtttccc agtcgtgcca tttcgcaaat
    37441 gcatcaggtg cttaatgatc ggcttgatga tgccattagc cacctgacgg acagcctcgc
    37501 accgctaccc gaaacgcgga ttgaaagaga ggcgctggcg ctgcaaaaac tcaatgtctt
    37561 ttgcctcgcg gacgatgcca actggcgaac ccagagtgca tggtggcaaa gctgcgtggc
    37621 aacggtaacc tacatttact cgacgctgaa tcgctacgat cccacctctt ttgctgattc
    37681 tcaggcaatt attgaattcc gacaaaaatt agcttcagaa atcaacaagc tgcagcatgc
    37741 cattaccgaa ggtcagtgct ggcaaagcga ctggcggatc agtgaaagtg aagcgatggc
    37801 ggcacgggaa tgtaacctgg agaatatctg ccagacgctg ttacagcttg gtcagatgga
    37861 cccgaatacg ccgccaacgc ccgcagccaa accgccatca atggccgccg atgcttttac
    37921 caatccagac tatatgcgct acgcggtaaa aacgctgctc gcctgtttga tctgtcacac
    37981 cttctacagc ggcgtggact gggaaggcat tcacacctgt atgctgacct gcgtgatcgt
    38041 cgctaatccg aatgtcggtt catcgtacca gaagatggtg ctgcgttttg gcggggcctt
    38101 ttgcggcgcg attctggcgc tgttattcac gctactggtc atgccctggc tggacaatat
    38161 tgtcgaattg ctgtttgtgc tggcaccgat tttcctgttg ggcgcatgga ttgccaccgg
    38221 ctctgaacgc tcttcttata tcggcacaca gatggtggtc accttcgcgc tcgccacgct
    38281 cgaaaacgtt tttggcccgg tgtacgacct ggtggaaatt cgcgatcgcg ccctgggtat
    38341 catcattggt accgtggtgt ccgcggtgat ttacaccttt gtctggcctg aaagtgaagc
    38401 gcgcacgctg ccgcaaaaac tgactgtcgc gctgggtatg ttaagtaaag taatgcggat
    38461 cccacgccag caggaagtca cggctctgcg cacttatctg caaattcgta taggtctgca
    38521 tgcggcgttt aatgcctgtg aagagatgtg ccaacgcgtg gcgctggagc gtcaactgga
    38581 cagcgaagaa cgcgccttac tgattgaacg ttcgcaaacg gttattcgtc agggccgcga
    38641 tcttcttcac gcctgggatg ccacctggaa ctcggcgcag gcgctggata acgcactaca
    38701 gccggacaga gcaggtcagt ttgccgacgc cctggaaaaa tacgctgccg gtctggcaac
    38761 cgcactcagc cgttctcctc aaataacgct tgaagagaca cccgcctctc aggccatcct
    38821 gcccacctta ttaaaacagg agcaacacgt ctgccagctt ttcgcccgct tgccagactg
    38881 gacagccccg gcattaacac ccgccacgga acaggtacaa ggagccacgc aatgatcaat
    38941 cgtcaacttt cacgtctgct gttgtgcagc attctcggca gcacgacgct gatttccggc
    39001 tgtgccctgg tacgtaagga ttctgcgcct catcaacagc tcaaaccgga acaaatcaaa
    39061 ctggccgatg atattcatct tgccagctcc ggctggccgc aggcgcagtg gtggaaacaa
    39121 ctcaatgacc cgcagttgga tgccctgatc cagcggacgc taagcggttc acacaccctc
    39181 gcagaagcga aactgcggga agaaaaagcg cagtcccagg ccgatttgtt agatgccggt
    39241 tcacagttgc aggtggcagc gttagggatg ctcaaccgcc aacgtgtctc ggcgaacggc
    39301 tttttaagcc cttatgcgat ggatgcgcca gcactgggga tggacgggcc gtactatacg
    39361 gaagccacag taggtttgtt tgccggactg gatcttgatt tgtggggtgt gcatcgctca
    39421 gcggttgccg ccgccattgg cgcgcataat gccgcgctgg cagaaaccgc agcagtagag
    39481 ctatcgctga ccacgggcgt agcgcagctt tattacagta tgcaggccag ctatcagatg
    39541 ctcgatctgt tagaacaaac tcgcgatgtg attgattacg cggtgaaagc gcaccaaagt
    39601 aaagtggcac acggtctgga agcgcaagtg cctttccacg gcgcgcgggc acagattctg
    39661 gcggtcgata aacaaattgc tgccgttaaa gggcaaatca ccgaaacgcg agaatctctg
    39721 cgcgcattga ttggcgcggg agccagcgat atgccggaga tcaaaccggt ggcattaccg
    39781 caagtccaga ctggcattcc ggcgacgctc tcttatgagt tgctcgccag acgcccggat
    39841 ctgcaagcca tgcgctggta tgttcaggcg tcattagatc aggtggattc cgcgcgggcg
    39901 ctgttctacc caagctttga tatcaaagcg tttttcggcc ttgatgccat ccacctggat
    39961 accttattca aaaaaaacag tcgccagttc aacttcatcc cgggtctgaa attgccgctg
    40021 tttgacggtg gacggttgaa tgccaatctc gaaggcactc gcgccgccag caacatgatg
    40081 attgaacgtt acaaccagtc agtactgaac gcggtgcgtg acgttgccgt caacggcacg
    40141 cgtctgcaaa cgctcaacga cgagcgagaa atgcaggctg aacgcgtgga agcaacgcgc
    40201 ttcacccagc gcgctgccga ggccgcctat cagcgcggct taaccagccg cttacaggcc
    40261 actgaagccc ggttgccagt acttgccgaa gagatgtcat tactgatgct ggacagccgc
    40321 cgggtgctcc aaagcattca gttgatgaaa tcgctgggcg gcgggtatca ggcaggtccc
    40381 gtcgtcgaga aaaaataaaa tgtctgccgc gtgatggctg tcacgcggta tttcgtttcg
    40441 tcacgtcaaa actgacgaca gcctgttttt cgtcagagtt ttgaataaat agtgcccgta
    40501 atatcaggga atgaccccaa ataaaatgtg gcataaaaga tgcatactgt agtcgagagc
    40561 gcgtatgcgt gatttgatta actggagcga gaccgatgaa aaaagtcgtc acggtttgcc
    40621 cctattgcgc atcaggttgc aaaatcaacc tggtcgtcga taacggcaaa atcgtccggg
    40681 cggaggcagc gcaggggaaa accaaccagg gtaccctgtg tctgaagggt tattatggct
    40741 gggatttcat taacgatacc cagatcctga ccccgcgcct gaaaaccccc atgatccgtc
    40801 gccagcgtgg cggcaaactc gaacctgttt cctgggatga ggcactgaat tacgttgccg
    40861 agcgcctgag cgccatcaaa gagaagtacg gtccggatgc catccagacg accggctcct
    40921 cgcgtggtac gggtaacgaa accaactatg taatgcaaaa atttgcgcgc gccgttattg
    40981 gtaccaataa cgttgactgc tgcgctcgtg tctgacacgg cccatcggtt gcaggtctgc
    41041 accaatcggt cggtaatggc gcaatgagca atgctattaa cgaaattgat aataccgatt
    41101 tagtgttcgt tttcgggtac aacccggcgg attcccaccc aatcgtggcg aatcacgtaa
    41161 ttaacgctaa acgtaacggg gcgaaaatta tcgtctgcga tccgcgcaaa attgaaaccg
    41221 cgcgcattgc tgacatgcac attgcactga aaaacggctc gaacatcgcg ctgttgaatg
    41281 cgatgggcca tgtcattatt gaagaaaatc tgtacgacaa agcgttcgtc gcttcacgta
    41341 cagaaggctt tgaagagtat cgtaaaatcg ttgaaggcta cacgccggag tcggttgaag
    41401 atatcaccgg cgtcagcgcc agtgagattc gtcaggcggc acggatgtat gcccaggcga
    41461 aaagcgccgc catcctgtgg ggcatgggtg taacccagtt ctaccagggc gtggaaaccg
    41521 tgcgttctct gaccagcctc gcgatgctga ccggtaacct cggtaagccg catgcgggtg
    41581 taaacccggt tcgtggtcag aacaacgtgc agggtgcctg cgatatgggc gcgctgccgg
    41641 atacgtatcc gggataccag tacgtgaaag atccggctaa ccgcgagaaa ttcgccaaag
    41701 cctggggtgt ggaaagcctg ccagcgcata ccggctatcg catcagcgag ctgccgcacc
    41761 gcgcagcgca tggcgaagtg cgtgccgcgt acattatggg cgaagatccg ctgcaaactg
    41821 acgcggagct gtcggcagta cgtaaagcct ttgaagatct ggaactggtt atcgttcagg
    41881 acatctttat gaccaaaacc gcgtcggcgg cggatgttat tttaccgtca acgtcgtggg
    41941 gcgagcatga aggcgtgttt actgcggctg accgtggctt ccagcgtttc ttcaaggcgg
    42001 ttgaaccgaa atgggatctg aaaacggact ggcaaatcat cagtgaaatc gccacccgta
    42061 tgggttatcc gatgcactac aacaacaccc aggagatctg ggatgagttg cgtcatctgt
    42121 gcccggattt ctacggtgcg acttacgaga aaatgggcga actgggcttc attcagtggc
    42181 cttgccgcga tacttcagat gccgatcagg ggacttctta tctgtttaaa gagaagtttg
    42241 ataccccgaa cggtctggcg cagttcttca cctgcgactg ggtagcgcca atcgacaaac
    42301 tcaccgacga gtacccgatg gtactgtcaa cggtgcgtga agttggtcac tactcttgcc
    42361 gttcgatgac cggtaactgt gcggcactgg cggcgctggc tgatgaacct ggctacgcac
    42421 aaatcaatac cgaagacgcc aaacgtctgg gtattgaaga tgaggcattg gtttgggtgc
    42481 actcgcgtaa aggcaaaatt atcacccgtg cgcaggtcag cgatcgtccg aacaaagggg
    42541 cgatttacat gacctaccag tggtggattg gtgcctgtaa cgagctggtt accgaaaact
    42601 taagcccgat tacgaaaacg ccggaataca aatactgcgc cgtgcgcgtc gagccgatcg
    42661 ccgatcagcg cgccgccgag cagtacgtga ttgacgagta caacaagttg aaaactcgcc
    42721 tgcgcgaagc ggcactggcg taatacagtc ctttctacag cctcctttcg gaggctgttt
    42781 ttttatccat tcgaactctt tatactggtt actccctacc caatcgtatt atcaaaatga
    42841 aaaaaattat cgcattgatg ttgtttttga cattctttgc ccacgcgaac gactccgagc
    42901 ctggcagcca gtatttaaag gcagcagagg ccggggaccg acgcgcacaa tattttcttg
    42961 ccgacagctg gtttagctcc ggcgatttga gcaaagccga atattgggca cagaaagccg
    43021 ccgacagcgg tgatgctgat gcctgcgcgc tgctggcgca gatcaaaatc accaatccgg
    43081 tcagtctgga ctatccacaa gcaaaagttc ttgcagagaa agcggcgcaa gcgggcagta
    43141 aagaaggtga agtaacgctg gcgcatattc tggtaaatac tcaagcgggc aaaccggatt
    43201 atccaaaggc gatttcgctg ttagaaaacg cctcggaaga tctggagaac gactctgccg
    43261 tcgatgccca aatgctgctt ggtttgattt acgccaacgg cgtgggcatt aaggccgacg
    43321 atgacaaggc aacctggtat ttcaaacgca gctctgcaat ttcccgaacc ggttattccg
    43381 agtactgggc gggaatgatg ttcttaaacg gtgaagaagg ttttatcgag aagaacaagc
    43441 aaaaggcgct gcactggttg aacctgagct gtatggaagg gtttgatacc gggtgtgaag
    43501 agtttgaaaa attaaccaac ggttaaggta ggcctgataa ggcgcgttaa gcgtcgcatc
    43561 aggc
//
LOCUS       DAZFKF010000033        42886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFKF010000033 DAZFKF010000000
VERSION     DAZFKF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.033"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(158..364)
                     /gene="ybfA"
                     /locus_tag="ACHKA1_002211"
     CDS             complement(158..364)
                     /gene="ybfA"
                     /locus_tag="ACHKA1_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO5679864.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            677..766
                     /gene="kdpF"
                     /locus_tag="ACHKA1_002212"
     CDS             677..766
                     /gene="kdpF"
                     /locus_tag="ACHKA1_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO5679865.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            766..2439
                     /gene="kdpA"
                     /locus_tag="ACHKA1_002213"
     CDS             766..2439
                     /gene="kdpA"
                     /locus_tag="ACHKA1_002213"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO5679866.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPFGSGLARLINDIPLPGTAGVER
                     VLFSALGVSDREMNWKQYLSAILCLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFSLIRAFTRQSM
                     STLGNAWVDLLRITLWVLAPVALLIALFFIQQGALQNFQPYQAVTTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2462..4510
                     /gene="kdpB"
                     /locus_tag="ACHKA1_002214"
     CDS             2462..4510
                     /gene="kdpB"
                     /locus_tag="ACHKA1_002214"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO5679867.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSVAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTCILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4519..5091
                     /gene="kdpC"
                     /locus_tag="ACHKA1_002215"
     CDS             4519..5091
                     /gene="kdpC"
                     /locus_tag="ACHKA1_002215"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO5679868.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5084..7768
                     /gene="kdpD"
                     /locus_tag="ACHKA1_002216"
     CDS             5084..7768
                     /gene="kdpD"
                     /locus_tag="ACHKA1_002216"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO5679869.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7765..8442
                     /gene="kdpE"
                     /locus_tag="ACHKA1_002217"
     CDS             7765..8442
                     /gene="kdpE"
                     /locus_tag="ACHKA1_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO5679870.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(8617..10257)
                     /gene="pgm"
                     /locus_tag="ACHKA1_002218"
     CDS             complement(8617..10257)
                     /gene="pgm"
                     /locus_tag="ACHKA1_002218"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO5679871.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(10283..10828)
                     /gene="seqA"
                     /locus_tag="ACHKA1_002219"
     CDS             complement(10283..10828)
                     /gene="seqA"
                     /locus_tag="ACHKA1_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO5679872.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            11013..11777
                     /gene="ybfF"
                     /locus_tag="ACHKA1_002220"
     CDS             11013..11777
                     /gene="ybfF"
                     /locus_tag="ACHKA1_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5679873.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPTMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFSAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            11917..12210
                     /gene="ybfE"
                     /locus_tag="ACHKA1_002221"
     CDS             11917..12210
                     /gene="ybfE"
                     /locus_tag="ACHKA1_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO5679874.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            12350..12880
                     /gene="fldA"
                     /locus_tag="ACHKA1_002222"
     CDS             12350..12880
                     /gene="fldA"
                     /locus_tag="ACHKA1_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO5679875.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            13090..13176
                     /gene="uof"
                     /locus_tag="ACHKA1_002223"
     CDS             13090..13176
                     /gene="uof"
                     /locus_tag="ACHKA1_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO5679876.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            13169..13615
                     /gene="fur"
                     /locus_tag="ACHKA1_002224"
     CDS             13169..13615
                     /gene="fur"
                     /locus_tag="ACHKA1_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO5679877.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(13699..14025)
                     /gene="chiQ"
                     /locus_tag="ACHKA1_002225"
     CDS             complement(13699..14025)
                     /gene="chiQ"
                     /locus_tag="ACHKA1_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO5679878.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(14075..15481)
                     /gene="chiP"
                     /locus_tag="ACHKA1_002226"
     CDS             complement(14075..15481)
                     /gene="chiP"
                     /locus_tag="ACHKA1_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO5679879.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(16060..17724)
                     /gene="glnS"
                     /locus_tag="ACHKA1_002227"
     CDS             complement(16060..17724)
                     /gene="glnS"
                     /locus_tag="ACHKA1_002227"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO5679880.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(17927..19873)
                     /gene="nagE"
                     /locus_tag="ACHKA1_002228"
     CDS             complement(17927..19873)
                     /gene="nagE"
                     /locus_tag="ACHKA1_002228"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO5679881.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAETAPATTAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            20206..21006
                     /gene="nagB"
                     /locus_tag="ACHKA1_002229"
     CDS             20206..21006
                     /gene="nagB"
                     /locus_tag="ACHKA1_002229"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO5679882.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            21066..22214
                     /gene="nagA"
                     /locus_tag="ACHKA1_002230"
     CDS             21066..22214
                     /gene="nagA"
                     /locus_tag="ACHKA1_002230"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO5679883.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            22223..23443
                     /gene="nagC"
                     /locus_tag="ACHKA1_002231"
                     /gene_synonym="nagR"
     CDS             22223..23443
                     /gene="nagC"
                     /locus_tag="ACHKA1_002231"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO5679884.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            23491..24243
                     /gene="nagD"
                     /locus_tag="ACHKA1_002232"
     CDS             23491..24243
                     /gene="nagD"
                     /locus_tag="ACHKA1_002232"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO5679885.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(24294..24392)
                     /locus_tag="ACHKA1_002233"
                     /pseudo
     CDS             complement(24294..24392)
                     /locus_tag="ACHKA1_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24640..26304
                     /gene="asnB"
                     /locus_tag="ACHKA1_002234"
     CDS             24640..26304
                     /gene="asnB"
                     /locus_tag="ACHKA1_002234"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO5679886.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            26684..26760
                     /locus_tag="ACHKA1_002235"
     tRNA            26684..26760
                     /locus_tag="ACHKA1_002235"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26718..26720,aa:Met,seq:cat)
     gene            26770..26854
                     /locus_tag="ACHKA1_002236"
     tRNA            26770..26854
                     /locus_tag="ACHKA1_002236"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26804..26806,aa:Leu,seq:tag)
     gene            26878..26952
                     /locus_tag="ACHKA1_002237"
     tRNA            26878..26952
                     /locus_tag="ACHKA1_002237"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26910..26912,aa:Gln,seq:ttg)
     gene            26968..27044
                     /locus_tag="ACHKA1_002238"
     tRNA            26968..27044
                     /locus_tag="ACHKA1_002238"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27002..27004,aa:Met,seq:cat)
     gene            27093..27167
                     /locus_tag="ACHKA1_002239"
     tRNA            27093..27167
                     /locus_tag="ACHKA1_002239"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27125..27127,aa:Gln,seq:ctg)
     gene            complement(27321..28496)
                     /gene="ubiF"
                     /locus_tag="ACHKA1_002240"
     CDS             complement(27321..28496)
                     /gene="ubiF"
                     /locus_tag="ACHKA1_002240"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO5679887.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRSKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            28642..30066
                     /gene="miaB"
                     /locus_tag="ACHKA1_002241"
     CDS             28642..30066
                     /gene="miaB"
                     /locus_tag="ACHKA1_002241"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO5679888.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            30219..31259
                     /gene="ybeZ"
                     /locus_tag="ACHKA1_002242"
     CDS             30219..31259
                     /gene="ybeZ"
                     /locus_tag="ACHKA1_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO5679889.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            31256..31723
                     /gene="ybeY"
                     /locus_tag="ACHKA1_002243"
     CDS             31256..31723
                     /gene="ybeY"
                     /locus_tag="ACHKA1_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO5679890.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            31813..32691
                     /gene="corC"
                     /locus_tag="ACHKA1_002244"
     CDS             31813..32691
                     /gene="corC"
                     /locus_tag="ACHKA1_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO5679891.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            32716..34254
                     /gene="lnt"
                     /locus_tag="ACHKA1_002245"
     CDS             32716..34254
                     /gene="lnt"
                     /locus_tag="ACHKA1_002245"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO5679892.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWSKTTWLRVAIVAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSTLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            34651..35559
                     /gene="gltI"
                     /locus_tag="ACHKA1_002246"
     CDS             34651..35559
                     /gene="gltI"
                     /locus_tag="ACHKA1_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO5679893.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            35729..36469
                     /gene="gltJ"
                     /locus_tag="ACHKA1_002247"
     CDS             35729..36469
                     /gene="gltJ"
                     /locus_tag="ACHKA1_002247"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO5679894.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            36469..37143
                     /gene="gltK"
                     /locus_tag="ACHKA1_002248"
     CDS             36469..37143
                     /gene="gltK"
                     /locus_tag="ACHKA1_002248"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO5679895.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            37143..37868
                     /gene="gltL"
                     /locus_tag="ACHKA1_002249"
     CDS             37143..37868
                     /gene="gltL"
                     /locus_tag="ACHKA1_002249"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO5679896.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            37986..38921
                     /gene="rihA"
                     /locus_tag="ACHKA1_002250"
     CDS             37986..38921
                     /gene="rihA"
                     /locus_tag="ACHKA1_002250"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO5679897.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQKC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHGEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            39005..40675
                     /gene="hscC"
                     /locus_tag="ACHKA1_002251"
     CDS             39005..40675
                     /gene="hscC"
                     /locus_tag="ACHKA1_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO5679898.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHTSAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIEIP"
     gene            complement(40735..42186)
                     /gene="djlC"
                     /locus_tag="ACHKA1_002252"
     CDS             complement(40735..42186)
                     /gene="djlC"
                     /locus_tag="ACHKA1_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFO5679899.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDDATEHEILRVFRALLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSVGMDEEEREAFLEAI
                     QAGDCFDFLGLLEYPVALQNQTVEYYFALERCCHYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWGSYPSTQADNLLLQWSAQHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(42183..>42886)
                     /gene="ybeU"
                     /locus_tag="ACHKA1_002253"
     CDS             complement(42183..>42886)
                     /gene="ybeU"
                     /locus_tag="ACHKA1_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO5679900.1"
                     /translation="KEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSSWG
                     INNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVAQ
                     TAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYVRAHHYYHSWMHYF
                     SAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPLT
                     VQPECPVTLKDVSDL"
BASE COUNT        10234 a        11084 c        11165 g        10403 t
ORIGIN      
        1 gtcagataga aactattagg aaaatattta tatatatttc atgaatatat gtttcgattg
       61 gtataacggt gtctaaatat tcctctgaac atcttcctga tacaaaaaac cgccagcgtt
      121 tccgctggcg gttttcaatg tattcagaac tcagcaatca ataaaaatca ccggttgcct
      181 tttcggcctg atccatccac accggtttat cgctggtttt cgaccagacg cgatgcaggt
      241 agctgtaaaa gcgggcgcgg tctttccaga aaagcatgaa aggcagcgcc agaacgcccg
      301 ctgcaaccgc ataagtacgg cgtaaaaaga taagccatgc aggatattct ctgtagagtt
      361 ccatactttt ctcccccttt tgtatctacc cggtgaatag caccggaaaa atgaatttgt
      421 ttatctgatg aaaatagtac cgccttttgt gtaattttac tactcatccg accacttatt
      481 tttgcttatt gatggtttat ttacattcac cctgtaatta agttacacaa aagttaaatt
      541 aatactaaac attagttaaa tcatggcttt tgccattttt atactttttt tacaccccgt
      601 ccgcagattt ttgcgaaatc tttgcagcca gaattctacc cttccggtat cacttttagg
      661 ccactggagg tgcactgtga gtgcaggcgt gataaccggc gtattgctgg tgtttttatt
      721 actgggttat ctggtttatg ccctgatcaa tgcggaggcg ttctgatggc tgcgcaaggg
      781 ttcttactga ttgccacgtt tttactggta ttaatggtgc tggcgcgtcc tttcggcagc
      841 gggctggcac ggttgattaa tgatattcct cttcccggta cagcgggcgt tgaacgcgta
      901 ctctttagcg cgcttggcgt ctctgaccgt gagatgaact ggaagcagta tctctcagcc
      961 attctctgtc tgaacatgct ggggctggcg gtgctttttt tcatgctgct cggtcagcac
     1021 tatctgccgc ttaatccaca gcagttgccg gggctgtcgt gggatctggc gctgaatact
     1081 gccgtcagct ttgtcactaa taccaactgg caatcttata gcggtgaaac cacgttgagc
     1141 tatttcagcc agatggcggg cttaacggtg caaaactttc tttctgccgc cagcgggatt
     1201 gcggtgattt tttccctcat ccgtgcattt acccgccaga gcatgagcac actcgggaat
     1261 gcctgggtcg atctgctacg catcacgtta tgggtgttgg cccctgtggc gttgttgatc
     1321 gcgctgtttt ttattcaaca aggtgcactg caaaattttc agccttatca ggctgtgacg
     1381 accgttgaag gagcgcagca gctgttacca atggggccag tggcttctca ggaagcgatc
     1441 aagatgctcg gcactaacgg cggtggcttc tttaatgcca actcgtcgca tccgtttgaa
     1501 aacccaaccg cactgaccaa cttcgtgcag atgctggcga tcttcttgat cccaacagcg
     1561 ctgtgctttg cctttggtga agtgacgggc gatcgccgcc aggggcgcat gttgctgtgg
     1621 gcgatgtcag tgatttttgt catctgcgta ggcgtggtga tgtgggcaga agttcagggt
     1681 aatcctcatc tgctggcgct cggcgcagac agcagcatca atatggaagg taaagagagc
     1741 cgtttcggcg tgctggtcag tagcctgttt gcggtcgtga ctacggcggc gtcctgtggc
     1801 gcggtgattg cgatgcatga ttcgtttacc gctctcggtg gcatggtgcc gatgtggctg
     1861 atgcaaattg gtgaagtggt attcggcggt gtcggttctg gtctttacgg catgatgctg
     1921 tttgtcctgc tggcggtgtt tattgccggg ctgatgattg gtcgtacacc ggaatatctg
     1981 ggtaaaaaaa tcgacgtacg tgagatgaaa ctgactgcac tggcgattct ggtcaccccg
     2041 acgctggtgc tgatgggcgc ggcgctggca atgatgaccg acgccggacg tagcgccatg
     2101 ctcaaccctg gcccgcatgg ttttagcgaa gtgctgtacg ccgtgtcatc cgccgctaac
     2161 aacaacggca gcgcctttgc cggattaagc gccaactctc cgttctggaa ctgtttactg
     2221 gcgttctgca tgtttgtcgg tcgcttcggg gtgattatcc cggtgatggc aattgccggt
     2281 tcgctggtga gtaaaaagag ccaacccgcc agctccggca cgctgccaac gcacggcccg
     2341 ctgtttgttg gcctgttaat cggcaccgtg ttgctggttg gcgcactgac ctttatccct
     2401 gccctggcgc ttggtccggt ggcggaatat ctctcctgat gatattgagt gagcactgaa
     2461 tatgagtcgt aaacaactgg cgctattcga accaacactt gtcgttcagg cgctgaaaga
     2521 agcggtgaaa aaattaaacc cgcaggcgca atggcgcaat ccggtgatgt ttatcgtctg
     2581 gatcggcagt ctgctgacca cctgtattag catcgcgatg gcaagcggcg cgatgcctgg
     2641 caatgcgctg tttagcgtgg ccattagcgg ctggctgtgg gtcaccgtac tgttcgctaa
     2701 tttcgccgag gcgctggcag aaggccgcag taaagcgcag gccaacagcc tgaaaggggt
     2761 gaaaaaaacg gcctttgccc gcaagctgcg cgaaccgaaa tatggcgctg cggcggacaa
     2821 agttcccgcc gatcaactgc gtaaaggcga tatcgtactg gtagaagcag gcgatattat
     2881 cccctgcgat ggtgaagtta ttgaaggcgg cgcatcggtc gatgaaagcg ccatcaccgg
     2941 ggaatcggca ccggtgatcc gtgaatccgg cggcgatttt gcctccgtca ccggcggcac
     3001 gtgtattctt tctgactggc tggtgattga gtgtagcgtt aaccccggcg agacatttct
     3061 ggatcggatg atcgcgatgg tggaaggcgc acagcgacgc aaaacgccga acgagattgc
     3121 cctgaccatc ctgctgattg ccctgactat cgtcttttta ctggcaaccg ccacgctgtg
     3181 gccgttttcc gcgtggggcg gtaatgcagt cagcgtaacg gtactggtgg cgctgctggt
     3241 ctgtctgatc ccaaccacta ttggcggcct gttgtcagcg atcggcgtcg ccgggatgag
     3301 tcggatgctg ggcgcgaatg tgattgccac cagcggacgt gcagttgaag cggcaggtga
     3361 cgttgacgtt ctgctgctgg ataaaaccgg caccatcaca ctcggtaacc gtcaggcatc
     3421 ggagtttatc cctgcgcagg gcgtggatga aaaaacgctg gctgacgccg cacaactggc
     3481 ttcgctggct gatgaaacgc cggaaggccg cagtattgtg atcctcgcca agcagcgttt
     3541 taacctgcgc gagcgcgatg tgcagtcgct ccatgccacc tttgtaccgt ttactgcgca
     3601 aagccggatg agcgggatca atatcgacaa ccgcatgatc cgtaaaggtt ctgtcgatgc
     3661 cattcgccgc catgttgagg ctaacggtgg tcacttccct gccgatgttg atcaaaaagt
     3721 cgatcaggtt gcgcgtcagg gggccacacc gctggtagta gtagaaggtt ctcgtgtgct
     3781 gggcgttatt gcgttgaaag atatcgtcaa aggcggcatt aaagaacgct tcgcccaact
     3841 gcgcaaaatg ggtattaaaa cggtgatgat taccggcgat aaccgtctga ctgccgccgc
     3901 cattgccgcg gaagcgggcg tcgatgattt tctcgccgaa gcgacaccgg aggccaagct
     3961 ggcattgatc cgtcagtatc aggcggaagg ccgtttggta gcaatgaccg gcgacggcac
     4021 caacgatgct ccggcgctgg cgcaggcaga tgtcgcggtg gcgatgaact ccggtactca
     4081 ggcggcgaaa gaagcgggca atatggtgga tctcgactct aacccgacca agttgatcga
     4141 ggtggtacat attggcaaac agatgctgat gacccgtggc tcgctgacca ccttcagcat
     4201 tgccaacgat gtggcgaaat acttcgccat tattccggcg gcattcgcgg taacgtatcc
     4261 acagttaaat gcgctgaaca tcatgcgcct gcattcgccc gactccgcca ttctcagtgc
     4321 ggtcattttc aacgccttga ttatcgtctt tttgattccc ctggcgttaa aaggcgtgag
     4381 ttataaaccg cttaccgctt ctgccatgtt gcgccgtaac ttatggattt acggtctggg
     4441 tgggctgctg gtgccgttta ttggtatcaa agtcattgat ttactactga ccgtttgcgg
     4501 tctggtgtga ggtttaccat gcgtggatta cgtccggcat tatcgacatt tatctttctg
     4561 ttattgatta ctggcggtgt ttacccgctg ctgaccaccg cgctggggca atggtggttt
     4621 ccctggcagg ccaacggttc gttgatccgt gaaggtgata cggtgcgggg ttcggcatta
     4681 atcgggcaga attttaccgg caacggctat tttcatggtc gcccgtcggc aacggcagaa
     4741 atgccttata atccacaggc ttctggcggg agcaatctgg cggtcagtaa ccctgagctg
     4801 gataaactaa tagccgcacg cgttgctgca ttacgggccg ctaacccgaa tgccagcacg
     4861 agcgttccgg ttgaactggt gacggcgtcg gcaagcgggc tggacaataa catcaccccg
     4921 caagcggcgg cctggcagat cccacgcgta gcgaaagcgc gtaatctcag cgttgaacag
     4981 ctcacgcaac tgatcgcaaa atacagccaa caaccgctgg tgaaatatat cggccagccg
     5041 gtcgtcaaca ttgtcgaact caatctggcg ctggataaac ttgatgaata acgaaccctt
     5101 acgtcccgac cccgatcgtc tgctggaaca aactgccgcg ccgcatcggg ggaagctgaa
     5161 agtttttttc ggtgcctgcg caggcgtcgg gaagacctgg gcgatgctgg cagaagccca
     5221 gcgactgcgg gcgcaagggc tggatattgt ggttggcgtg gtagaaaccc acgggcggaa
     5281 agataccgcc gcaatgctgg aagggctggc tgttctgccg ctgaaacgcc aggcgtaccg
     5341 tgggcggcat atcagcgagt ttgatctcga tgccgccctc gcccgccgtc cggcgctgat
     5401 cttaatggac gaactggcgc acagtaatgc gccaggttcc cgtcatccca aacgctggca
     5461 ggatatcgaa gaactgctgg aagctggcat tgatgttttc acaaccgtca acgttcagca
     5521 tctggaaagt ctgaatgatg tggtcagcgg cgtcaccgga attcaggtac gggaaaccgt
     5581 gcccgatcct tttttcgatg ccgccgacga cgtggtgctg gtggacttgc ccccggacga
     5641 tctgcgccag cggctaaaag aaggcaaagt ctatattgcc gggcaggcgg agcgcgccat
     5701 tgaacatttt ttccgcaaag gcaatctgat cgccctgcgc gaactggcac tgcgccgtac
     5761 tgccgatcgc gttgatgagc aaatgcgcgc ctggcggggg catcctggcg aagagaaagt
     5821 ctggcacacg cgcgacgcga tccttttatg catcggccat aacaccggca gcgaaaaact
     5881 ggtccgcgcg gcggcgcggc tggcgtcacg gctggggagc gtctggcacg cggtgtatgt
     5941 tgaaacccct gccctgcatc gcttaccgga gaaaaaacgt cgggcaattc tcagcgcctt
     6001 acgtctggcg caggagctgg gcgcggagac ggcaacactt tctgatccag cggaagagaa
     6061 agcggtagtg cgttatgccc gtgaacataa tctcggcaag attattctcg gtcgcccagc
     6121 ctcgcgccgc tggtggcgtc gggaaacgtt tgctgaccga ctggcgcgca tcgcccccga
     6181 tctcgatcag gtgctggtcg cgcttgatga accacccgcc cgcacgatta acaacgcgcc
     6241 ggatagccgc tcttttaaag acaagtggcg ggtacagatt cagggatgcg tggttgccgc
     6301 cgcgttatgc gccgttatca ccttaattgc catgcagtgg ctgatggcgt ttgatgccgc
     6361 caacctggtg atgctgtatc tacttggcgt ggtggtggtg gcgctatttt atggacgctg
     6421 gccttcggtg gttgctaccg tcattaatgt ggtgagtttc gatctctttt ttatcgcccc
     6481 acgcggcacg ctcgccgtct ctgatgtgca atatctgctg accttcgcgg tgatgttaac
     6541 cgtcgggctg gtgatcggga accttactgc gggtgtgcgt tatcaggcac gggtagcccg
     6601 ttaccgcgag caacgcacac ggcacttata tgaaatgtcg aaagctctgg cggtgggccg
     6661 cagtccgcag gacatcgccg ccaccagcga acaatttatt gcctccacgt ttcatgcccg
     6721 cagtcaggtg ttgttgcccg atgacaacgg taaattgcag ccgttaacac atccgcaagg
     6781 aatgacgccg tgggacgatg ccatcgcgca gtggagtttt gataaaggcc tgcctgcggg
     6841 tgcgggcacc gacacgttac ccggcgtgcc gtaccagatt ttgccgttaa aaagcggtga
     6901 gaaaacctac gggttggtgg tggtggaacc ggggaatctg cgtcagttga tgatcccgga
     6961 acagcagcgc ctgctggaga cgtttacgct gttagtcgcc aatgcccttg agcggctgac
     7021 gctgaccgcc agcgaagaac aggcgcggat ggcaagcgaa cgtgaacaga tccgcaacgc
     7081 cctgctggcg gcgctttcgc atgatttacg cactccgctt acggtgctgt ttggtcaggc
     7141 agaaatctta acgctcgatc tggcaagcga aggatcaccc cacgcccgcc aggccagcga
     7201 gattcgtcag catgtgctga acactacccg actggtgaat aatctactgg atatggcgcg
     7261 aattcagtcc ggcggcttta atttgaagaa agagtggtta acgctggaag aagtagtcgg
     7321 cagcgcgctg caaatgctgg aaccgggttt atcgtcgccc atcaatcttt ctctgccaga
     7381 accgctgacc ttaatccacg ttgacgggcc actctttgaa cgggtgctga ttaatctgct
     7441 ggagaacgcg gtgaaatatg cgggtgcgca ggccgaaatt ggtatcgatg cccacgttga
     7501 gggcgaaaat ctacaactgg atgtctggga taacggcccc ggtcttccgc caggccagga
     7561 gcagacgata tttgataagt ttgctcgcgg gaataaagag tcggcagtac cgggggtagg
     7621 gcttggactg gcaatttgtc gggcgatagt ggatgtacac gggggcacta ttaccgcgtt
     7681 caaccgaccg gaaggtggtg cctgttttcg tgttacactt ccccagcaaa ctgcccctga
     7741 acttgaagaa tttcatgagg atatgtgaca aacgttctga ttgttgaaga tgaacaggct
     7801 attcgtcgct ttctgcgcac ggcgctggag ggcgacggga tgcgcgtcta tgaggccgaa
     7861 acgctgcaac gcggcttgct ggaagcggct acccgtaagc cagatttgat tattctcgat
     7921 ctcggcctgc ccgatggtga tgggattgag tttatccgcg acctgcgcca gtggagcgcg
     7981 gtgccggtga ttgtgctttc cgcacgtagc gaagagagcg acaaaatcgc cgcgctggat
     8041 gccggagcag atgattatct gagtaagccg tttggcattg gcgaattgca ggcccgtctg
     8101 cgcgtcgcat tacgccgcca ctccgccacc gccgcgcccg atccgctggt aaaattttcc
     8161 gatgttaccg tcgatttagc cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc
     8221 acgccgattg aattccgcct gctggcggtg ctgctcaaca atgctggaaa agtgctcacc
     8281 cagcgccagc tccttaacca ggtatgggga ccaaacgcgg tcgaacacag tcattatttg
     8341 cggatttata tggggcatct gcgacaaaaa ctggaacagg atcccgctcg cccccgccat
     8401 ttcattactg aaaccggtat tggttatcgg tttatgcttt aggcggttaa atgctggatc
     8461 cggcatgaac gccttatccg gcctacaaaa tcgcgtaaat tcaatatatt gcaccatcct
     8521 cgtaggcctg ataagcgtag cgcatcaggc aattctgtgt ttgtcatacg taaaaaaggg
     8581 cgatcttccg accgcccttt ttcttattta atgtgtttac gcgtttttca gaacttcgct
     8641 aacaatctca accgcttctt tctcaatctg cttgcgatgt tcttcaccga ggaagctttc
     8701 gcagtagatc ttatatgcgt cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc
     8761 agtcatcact ttcagaccgc caatagaagc accgttgccc ggagcagcag tcaggcgcgc
     8821 ggtgatcggg tcacctgcca gggtgctggc gctcaccatt tccggagaca gcttagacag
     8881 cgccgctttt tgtgcggaag tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa
     8941 gcgttttgcc agttcgttgt agtgttcctg cgggttctta ccggtgacag cggtgatttc
     9001 cgccgccagc agacacatga tgatgccgtc tttgtcggtg gaccacggcg tgccgtcgaa
     9061 acgcaggaag gaagcccctg cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag
     9121 accatcaaca aaccatttga aacctaccgg gacttctacc agcttacgac ccaagtcatt
     9181 gaccacacgg tcgatcatcg cagaagaaac cagcgtttta ccaacggcaa catctttgcc
     9241 ccactgcgga cgatgctgga acaggtagtt gattgccacc gccaggtagt ggttcggatt
     9301 catcaaacct gccggagtga cgataccgtg acggtcataa tccgggtcgt tagcaaacgc
     9361 cagatcgaac ttatcacgca gtgccagcag gcccgccatc gcacactcgg aggagcagtc
     9421 catacggatc gcgccgtctt tatcaaggtg cataaagcgg aaggtttgat cgacctgatc
     9481 gttaacgata gtcaggttga ggttgtaata ctcgccaata cgcttccagt attcgatacc
     9541 ggaaccgccc agcggatcaa cgccaagcgt caggcccgct ttctgaatcg ccgccatatc
     9601 aacgatatcg gccagccctt ccacaaacgg ctgcaccaga tcctgctctt tcacatgacc
     9661 ggatgccatc gcttcgtcga gggagatacg cttcacgcct ttcaggccat cggccatcag
     9721 tgcgttggct ctgtcttcca ccactttagt gacgttggta tctgccgggc caccatttgg
     9781 cggattgtac ttgataccac catcttccgg cgggttatgg gacggtgtaa tcacgatacc
     9841 gtctgccagc gggccacctt ttttattgtg aaccaggatg gcattggaaa cggcaggcgt
     9901 cggggtgaag ccattgtttt cctgcacaat aacatctacg ccgttcgctg ccagcacttc
     9961 cagtacggaa ataaatgcag gttcggacag ggcgtgagta tctttaccca catagcaagg
    10021 gccagtgatg ccgtttttcg cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg
    10081 cggctcgtta aagctgtggc gcgctgcact gccacggtga ccggaagtac cgaatttcac
    10141 cgcgtgctcc gcattccctg cttctggttt cagtacataa tattgcgccg tcagttgggc
    10201 gacgttaatc aaatcactct gttgtgcagg ttggcctgca cgattgtgga ttgccattgc
    10261 tttgtccttt gtctgcaacg ttttagatag ttccgcaaac cttctcaatc aattccgccg
    10321 ggaattgcat cgactgcatg atgtgttcga tcatgctgca tttacggccg gtgttggtgt
    10381 tggtgatcac ccaatacggc gtgcctggca catgtttcgg cttggtctga ttaccatttt
    10441 tcagcagcgt ttgttcatct gccgcaaagt aaacgcgtgt acgaccgtgc aacgattccg
    10501 ttgcttcggc aaacgcctgg gcgtcaagag aatatagtgt agacaacagc agcataaagc
    10561 gattgaccgc tcgcttttgc tctgcgtatt catccgaaag cagaagttca cgcattgcgc
    10621 gaaccttgtc tttaatcgtt ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc
    10681 gaacctcttt cgtcaccgga gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc
    10741 gtaaaatgtc ggatgcgctc tcgccgatat gcttagtgtg gctggcaata tagctgtaga
    10801 gttcatcatc aacttcaatc gttttcatct taatccagtg cagtgtctta gctgaataca
    10861 agtcgccagg agtatatgaa taaatcccgg cagcggatag cgtcaagcct ggcttgcaga
    10921 aaaactcgga ataaacctta tttgttgcag ccctgtggca caatggtcaa cataataccc
    10981 taacccgaca acgcgaaaaa aagaactttg ccatgaaatt gaatatccgc gcgcaaactg
    11041 cacaaaacca gcacaataat tctcccatcg ttcttgtcca tggtctgttt ggcagcctcg
    11101 acaaccttgg cgtactggct cgcgatctgg taaacgatca caatatcatc caggttgata
    11161 tgcgtaacca cggtctttca ccgagagatc cggtaatgaa ttacccgacg atggcgcagg
    11221 atcttgttga taccctggat gcactgcaga tcgacaaagc aacatttatc ggtcactcca
    11281 tgggcggtaa agcggtaatg gcacttaccg cactggcccc cgatcgcatc gataaactgg
    11341 tggcgatcga tatcgcaccg gtcgactatc acgtacgccg tcatgatgag attttctcgg
    11401 ctatcaacgc ggtcagtgaa tcggacgcac aaactcgcca gcaagcagca gcaataatgc
    11461 gccagcatct taatgaagaa ggggtgattc agtttctgtt gaaatctttt gttgacgggg
    11521 agtggcgctt taacgtgccg gtattgtggg atcagtatcc gcatattgtg ggttgggaga
    11581 aaatcccggc atgggatcac cctgccctgt ttatccctgg cggcaattct ccgtatgtta
    11641 gcgagcagta ccgtgatgat ttactggcac aatttccaca ggcacgggcg catgtgattg
    11701 caggcgcggg tcactgggtc catgctgaaa aaccagatgc ggtattacgc gctatccgtc
    11761 gctatctcaa tgattaacgc aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg
    11821 tgggcgttgg cgagactcct ctcgctgttg tattatggcg cgctatccat ccgggcaggc
    11881 gcctggctga ttgtttcccc cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt
    11941 acgacattag atctgttcgc gcacgagcgt cgaccgggac gaccgaaaac taatccgctt
    12001 tcgcgcgatg aacagctgcg tattaataaa cgcaaccaac taaaacgcga caaagtacgt
    12061 ggccttaagc gtgtcgaact gaagctgaac gcggaagctg tcgaggcgct gaacgaactg
    12121 gcggaatcgc gcaatatgag ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg
    12181 gcggcgctgc gtagtcaggg catcgtttaa atttccactt tcatgtagca cagtgtgcag
    12241 tcctgctcgt ttgctgattc cgcaagactg cctgttctgc tatgattgcc tttatccgtg
    12301 ggcaattttc cacccccatt tcaataagtt tcaagaggtt atttcactca tggctatcac
    12361 tggcatcttt ttcggcagcg acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa
    12421 acagcttggt aaagacgttg ccgatgtcca tgacattgca aaaagcagca aagaagatct
    12481 ggaagcttat gacattctgc tgctgggcat cccaacctgg tattacggcg aagcgcagtg
    12541 tgactgggat gacttcttcc cgactctcga agagattgat ttcaatggca aactggttgc
    12601 gctgtttggt tgtggtgacc aggaagatta cgccgaatat ttctgcgacg cattgggcac
    12661 catccgcgac atcattgaac cgcgcggtgc aaccatcgtt ggtcactggc caactgcggg
    12721 ctatcatttc gaagcatcaa aaggtctggc agatgacgac cactttgtcg gtctggctat
    12781 cgacgaagac cgtcagccgg aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat
    12841 ttctgaagag ttgcatctcg acgaaattct caatgcctga tgtgatgcgg cgtagactca
    12901 tgtctacgcc gtattaatag ataatgccaa tcaaaataat tgctacaaat ttgtaacttt
    12961 tgctgttgta cctgtacaat gtcccggtgt tcaagtggcc ttgccgttgt aaatgtaagc
    13021 tgtgccacgt ttttattaac aatatttgcc agggacttgt ggttttcatt taggcgtggc
    13081 aattctataa tgatacgcat tatctcaaga gcaaattctg tcacttcttc taatgaagtg
    13141 aaccgcttag taacaggaca gattccgcat gactgataac aataccgccc taaagaaagc
    13201 tggcctgaaa gtaacgcttc cacgtttaaa aatcctggaa gttcttcagg agccggacaa
    13261 ccatcacgtc agtgcggaag atttatacaa acgtctgatc gatatgggtg aagaaattgg
    13321 tctggctacg gtatatcgcg tactgaacca gtttgacgac gctggtatcg tcacccgcca
    13381 caattttgaa ggcggtaaat ccgtatttga actgacacag caacatcacc acgatcacct
    13441 gatctgcctc gactgcggca aggttatcga atttagtgat gattcaatcg aagcgcgtca
    13501 gcgtgaaatt gccgcaaaac atggcattcg cctgactaac cacagtctct atctttacgg
    13561 tcactgtgcc gaaggcgatt gccgcgaaga tgagcacgcg cacgaaggca aataagccag
    13621 tctgaacgag aaaagccaac ctgcgggttg gcttttttat gcaagggaaa taagaaaagc
    13681 gagagttaca gctctcactt atttgttatt actgcgaatt tcctgccaga ccttatcaca
    13741 atcggctttc accgcctgat catttccggt ttgcgttgca cgtagacact gctgataatc
    13801 cagtacgcgg acactttcct ggtcggcaaa ctgctgatgt tgtttctctt tcttcagcac
    13861 gtttaacacg ctctggcagg cttcaatttt ttccggcgaa ccttgtgcgg tgttgataca
    13921 ggcgctgtat gcctctttca gacggctgtc ttcctgtggt gcggttgatt gcgcacaagc
    13981 taccagcccc gatgccatca tggcgatgag aatcagtttt ttcatagcaa attcctcaaa
    14041 cgtgcggcag gcacaaaacc tgccgcgtcg ggcatcagaa gatggtgaat ggtgcgatta
    14101 ccataaattt cacgtcacgc tcatcctgga agatgttacc gtaaccgccg ccccagcttg
    14161 ggatgtcgga gtggttgtcg tattcggtga agtgcagttt gaacatcgtg cctttggcgc
    14221 gaccgtcctg aatggtgtag accgcatcca ggctgtaggc agactcttca atagtccggt
    14281 ttttgtcgta gtacgcatcc ggattgctct gccaggtcgc aggtttagca tcccatgcgt
    14341 aaacgtagga agcgccgatg gcgaagcctg gaagattcca gtttttcagg tcatacatcg
    14401 caccgaagaa gaccgctttt tcgccgttgg cgttgaagtc agaacggtta tcccaccaga
    14461 tatccaggcg accgttcgag gaagcgtagg ttggagtcat acgttgcagg aagtatccct
    14521 gctgaccgtc agctttaacc caggtgcctt cgaggcgcaa atccactacg tcagccgccc
    14581 ggtaaccaaa ggtcaacgct tgcagccagg cggtgccgtc ataaagatcg ttgacgctgc
    14641 gatcgtcaac tttatcgcgc gtaccgtaga actggtagct ggtggttaac gggctaccgg
    14701 cgatatcaaa tttgtagctg gctttggcaa agtattgatc gatataccct tccgcctgac
    14761 caaatgccgc ttccagtacg aagttatttt tgaagtcgta tttcgcaccg atggagtgca
    14821 gataatcaac tttggtagtt ttatcgttct gataaaactc atccatttcc agatgccacg
    14881 gtgctttgta ttcgttggtc cacatgtagg agaaactcaa cgcaccggca tcgccgtaat
    14941 caaaattcgc tccggcttcc gcaccctgat aggtacctgg cataaagctc cagtgcggcg
    15001 ctaacagcgt ctgaccggtt ggctgaatat aacctgccct cgcccaaacc ggaccgtatt
    15061 taaatttggc cgctgcttta tacaggctta taccgctttt atcgccggac cagtcttcgt
    15121 cataggcttt attacttttt gaaaacgcga tttcgttcgg gtgagagctg tcgccgtttt
    15181 ccgccatttc aatcgccgta aacgcggcaa tatcaaggcc gaacatatca gcagcataac
    15241 cagactgaaa atcgaggttg gcattccagg tggagtgaga aaggttggtt ttgtatttgt
    15301 cgccgtcggt aacatcttta cggtcgcgtt cacgctgcca gtaatagata ccgccggtta
    15361 aggttgaatc gtcgatgaat ccttcggcgc gagcctccgg cattgccata aagcccgaca
    15421 ttgctgtaac accggcgata gccagcgcca gcgtactacg tttgccacta aacgtacgca
    15481 tgggttaatc ctctttgacg tataaattgc tgcaccaaag gtgaacagcg aaaaaataaa
    15541 aattaaaaaa gtagcgttga gaaaagtctc gctgactaca ggaacagact atttttcgcg
    15601 gcgcgaatta tcaatctctt tctgtaacca gaatgtaaga acatgagcaa ccgcacatat
    15661 tttgatgatt tttgttacaa ggcttaagtt tgcggttgaa aggcttgcag aagaataaaa
    15721 gcgtgaattt ccagcaagat gaaagcggtt acattttatt taattatgtt ttgttatttt
    15781 aaatttattt tatggataaa ttgcgaagcg gaattaattc gtgacgaaaa gcagaaaaaa
    15841 acgccgcaga ccgcggcgtt taggattgtt tgcatgtctc ttgccggatg cggcgtgaac
    15901 gccttatccg gcctacaaaa gcacgcaaac tcaatatatt gcagagatca tgtaggcctg
    15961 ataagcgtag cgcatcaggc aatttagcgt ttgaaactgt ttgtctgttc cttgccggat
    16021 gcgacgtaaa cgccccatcc ggcatagcga aacttaaaat tactcgccta ctttcgccca
    16081 ggtatcacgc agcccaacgg tgcggttaaa taccggtttt tccgccgtag aatggcggct
    16141 atcgaggcag aagtaacctt cacgctcaaa ctggaatgct ttacccgcta ccgcatcttt
    16201 cagcgacggt tcagcaaagc cctgtttgat caccagcgat tccgggttaa tcaccgacag
    16261 gaaatcatcc gcagcacctg ggttaggcac gctgaacaga cgatcataca aacggatttc
    16321 aaccggcagc gcatgtgccg cgctcaccca gtgaataacg cctttgactt tacgaccatc
    16381 agccggatct ttgcttaagg tgtcggcgtc ataagtacag aagatggtgg tgatattacc
    16441 ttcggcatct ttctcgacgc gttctgcctt aatcacataa gcattacgca gacgcacttc
    16501 tttaccgagc accagacgtt tgtactgctt gttagcttct tcgcggaaat cggcgcgatc
    16561 gatccaaatc tcaccgctaa acggcacctg acggctgccc atttccggtt tgttcggatg
    16621 gttcggcatg gtaaccattt cgccttcgcc ctgatagttt tcgataacca gtttcaccgg
    16681 atcgataacc gccattgcgc gcggcgcatt ttcgttgaga tcttcacgga tgcaggattc
    16741 cagcgacgcc atctcaatgg tgttgtcctg cttggtcacg ccgatgcgtt tgcagaactc
    16801 acgaatagaa gccgcagtgt aaccacgacg acgcagaccg gaaatggtcg gcatacgcgg
    16861 gtcatcccag ccttcaacgt gcttatcggt caccagcagg ttcagcttac gcttggacat
    16921 cacagtgtat tccagattca ggcgcgagaa ctcatactgg cgcgggtgaa caggaatggt
    16981 gatgttgtcc agtacccagt cgtacagacg acggttgtcc tggaactcca gcgtacacag
    17041 agagtgcgta ataccttcca gcgcatcgct gatgcaatgg gtgaagtcgt acatcgggta
    17101 gatgcaccac ttgttgccag tctggtggtg ttcagcaaat ttaatgcggt acagcaccgg
    17161 atcgcgcatc acgataaacg gtgaagccat gtcgattttc gcacgcaggc aggctttacc
    17221 ttcttcaaaa ccgccggtac gcattttctc gaacagcgcc aggttctctt caacgctacg
    17281 gtcgcggtac gggctgtttt taccaggctg cgtcagggtg ccgcggtatt cgcggatctg
    17341 ttccggcgtc agttcatcaa cgtacgccag gcctttattg atcagttcga tcgcataggc
    17401 gtggagctga tcaaaataat cggaggagta acggacgtta ccagaccagt gaaaacctaa
    17461 ccactcaacg tcgtttttga tcgaatcgac atactcgata tcttctttta ccgggttagt
    17521 gtcgtcgaaa cgcaggttgc actggccttt atagtcctgg gcgatcccga agttcaggca
    17581 gatagatttc gcatggccaa tatgcagata gccattcggc tccggcggga aacgggtgtg
    17641 tactgtggtg tgcttaccac tggccagatc ttcatcgatg atctgacgga taaagttagt
    17701 cgggcgggct tctgcctcac tcatcgtgga ttcctcaaag cgtaaacaac gtataacggc
    17761 gtatgatctt ataagcggga caggctgaca actgttagtt agtgaaaatc gaaaatctca
    17821 ctgagattat ggggatgatt gatgaaaaaa aaagcggcag agaagatctc caccgcttgt
    17881 cattgttgga tgcgacgctc aagcgtcgca tcaggcataa agcagattac tttttgattt
    17941 catacagcgg tgtttgaccc gccacaacat ggccctgagc tttaatgatc aagccactga
    18001 aatcgtcgat attactgcaa accactgggc taatcatcga gcgggcgtta gcgttcaggt
    18061 aatccagatc catttccaga atcggttgtc ctgcgcttac ctgcgcgccc tcttccacca
    18121 gacgtttaaa gcctttacct tccagcgcta cggtgtcgat acccatatgg acgacgatct
    18181 ccgcgccttt ttcggtttcc agacagaacg cgtggttggt gttgaagatt ttcacgattg
    18241 tccctgcggc tggtgatacg acgattttat ctgtcggttt caccgccaca ccgtcaccca
    18301 ccgctttgct ggcgaatgct tcgtcaggaa cctgatccag tgccacgaca tcaccggtaa
    18361 tcggcgatac cagctccgcg atagataccg cgtttggtac agcctgcggt tttgctacag
    18421 gagcggtagt tgccggagca gtttcagcag aggcagctgc taccggaccg cgggcaacga
    18481 ctttcttcat cgcatcgccg atggattctg ctttcgcgcc aacaatcacc tgaatagtct
    18541 gtttgttcag ttttactacc ccagaagccc ccagacgttt acacatcgta tcgttaacgc
    18601 gggcagagtc agccacggta aggcgcagac gggtgataca ggcgtcaatt gctttcaggt
    18661 tgtcagtgcc gccaaccgca gcaatatagt tggtcgccag ttgattgagg ccttcttcag
    18721 tgttgctgtt ggcttcttca gtaacgatct cgtcttcttt atcttcacga cccggcgttt
    18781 tcaggttgaa catgcggata accaaactga acaccacgaa gtagatagcg aagaagacaa
    18841 cacccataac cagcagcatc cagacgttct ggctggcggc cggcaggtta tacatcaacg
    18901 cgtagtcgat agcccccgca gagaaagaga agccggcatg gatacccagc agcgttgcca
    18961 caaacaggct gataccggtc agcagtgcgt gcaggaggta cagcagcgga gcaaggaaca
    19021 tgaacaggaa ttccagcggc tcagtcacac cggtcaggaa cgcagtaaca gcaacagaaa
    19081 gcagcatccc gccaaccatc ggacgacgct ctttcggtgc tgcgaagtac atcgccagcg
    19141 ccgcacccgg caggccgaac atcatgatcg ggaagaagcc ggacatgaac atccccgcgg
    19201 tgccgtcacc agcgtagaaa cggttgatgt cgccgtggaa aaccgtaccc gccgcgttgg
    19261 tgaattcacc aatctggaac caggcgatgg tgttcagcac ctgatgcaga ccggttggga
    19321 tcagcagacg gttgatgaaa ccaaagatac cggaacccag cgcgcccgca gaaacgatcc
    19381 actcgccgcc tgcatggata gcgtgctgta ccggcggcca gacgtaacca aaaatggccg
    19441 ccagcaccag acagaagaag ccggtggcga tcggcacaaa gcgtttgccg ccgaagaagc
    19501 tcaggaagtc cggcagttta atatcggacc aacggttata ggctgcgcca ccaaccagac
    19561 cggtaatgat acccgccagt acacccatgt taatttctgg gttgatggtc accatcgctt
    19621 tggttaacac aaagtaacct accgcacccg ccagcgccgc cgcacctgcg ctgtctttcg
    19681 accagctgga tgccacaccg atggcgaaga ttaatgcgag gttatcaaaa atcgcaccgc
    19741 ccgcctgggc aataaacgca acgttaagta aatctggctg accgaatcgc agcaacagtg
    19801 ccgccaccgg cagcaccgcg atagggagct gtaacgccct accgagtcgc tggaaaaaac
    19861 ctaaaatatt catcttattc cccctacgag aaccctattt ggctcgttta aagccgtatt
    19921 tttattgtgc tgcaaattgt actgccgatg ttctgtaatc agattgttag atcatctgcc
    19981 acagagtgtg tgaaaattta attcgtatcg caaattaaac gcgtgtcttt tgtgagtttt
    20041 gtcaccaaat atcgttatca tcactccctt ttactggcta aaccagaaaa cttattttat
    20101 cattcaaaaa atcaggtcgg attgacgcct gtctgcgcaa atccaggtta cgcttaaaga
    20161 tgcctaatcc gccaacggct tacattttac ttattgaggt gaataatgag actgatcccc
    20221 ctgactaccg ctgaacaggt cggcaaatgg gctgctcgcc atatcgtcaa tcgtatcaat
    20281 gcgttcaaac cgactgctga tcgtccgttt gttctgggcc tgccaactgg cggcacgcca
    20341 atgaccacct ataaagcgtt agtcgaaatg cataaagcag gccaggtcag ctttaagcac
    20401 gttgtcacct tcaacatgga cgagtatgtc ggtctgccga aagagcatcc ggaaagctac
    20461 tacagcttta tgcaccgtaa tttcttcgat cacgttgata ttccagcaga aaacatcaac
    20521 cttctcaacg gcaacgcccc ggatatcgac gccgagtgcc gccagtatga agaaaaaatc
    20581 cgttcttacg gaaaaattca tctgtttatg ggcggtgtag gtaacgacgg tcatattgca
    20641 tttaacgaac cggcgtcttc tctggcttct cgtactcgta tcaaaaccct gactcatgac
    20701 actcgcgtcg cgaactctcg tttctttgat aacgatgtca atcaggtgcc aaaatatgcc
    20761 ctgactgtcg gtgttggtac actgctggat gccgaagaag tgatgattct ggtgctgggt
    20821 agccagaaag cactggcgct gcaggccgca gttgaaggtt gcgtgaacca tatgtggacc
    20881 atcagctgtc tgcaactgca tccgaaagcc atcatggtgt gcgatgagcc ttccaccatg
    20941 gagctgaaag ttaagacttt aagatatttc aatgaattag aagcagaaaa tatcaaaggt
    21001 ctgtaattgt tatccctgcc ctgacacctt gctcagggca atatttttta aaatcggggg
    21061 tcagaatgta tgcattaacc cagggccgga tctttaccgg ccacgaattt cttgatgacc
    21121 acgcggttgt tatcgctgat ggcctgatta aaagcgtctg tccggtagcg gaactgccgc
    21181 cagagatcga acaacgttca ctgaacgggg ccattctctc ccccggtttt atcgatgtgc
    21241 agttaaacgg ctgcggcggc gtacagttta acgacaccgc tgaagcggtc agcgtggaaa
    21301 cgctggaaat catgcagaaa gccaatgaga aatcaggctg tactaactat ctgccgacgc
    21361 ttatcaccac cagcgatgag ctgatgaaac agggcgtgcg cgttatgcgc gagtacctgg
    21421 caaaacatcc gaatcaggcg ttaggtctgc atctggaagg cccgtggctg aatctggtga
    21481 aaaaaggtac ccataatccg aattttgtgc gtaagcctga tgccgcgctg gtcgatttcc
    21541 tgtgtgaaaa cgccgacgtc attaccaaag tgaccctggc accggaaatg gttcctgcgg
    21601 aagtcatcag caaactggca aatgccggga ttgtggtttc tgccggtcac tccaacgcga
    21661 cgttgaaaga agcgaaagcc ggtttccgcg cggggattac ctttgccacc catctgtaca
    21721 acgcgatgcc gtatattacc ggtcgtgaac cgggcctggc gggcgcgatc ctcgatgaag
    21781 ctgacattta ttgcggtatt atcgctgatg gcctgcatgt tgattacgcc aacattcgta
    21841 acgctaaacg ccttaaaggc gacaaactgt gtctggttac cgacgccacc gcgccagcag
    21901 gtgccaacat tgaacagttc atttttgcgg gtaaaacaat atactaccgt aacggacttt
    21961 gtgtggatga gaacggtacg ttaagcggtt catccttaac catgattgaa ggcgtgcgta
    22021 atctggtcga acattgcggt atcgcactgg atgaagtgct gcgtatggcg acgctctatc
    22081 cggcgcgtgc gattggcgtt gagaaacgtc tcggcacact cgccgcaggt aaagtagcca
    22141 acctgaccgc attcacacct gattttaaaa tcaccaagac catcgttaac ggtaacgagg
    22201 tcgtaactca ataagagaaa gtatgacacc aggcggacaa gctcagatag gtaatgttga
    22261 tctcgtaaaa cagcttaaca gcgcggcggt ttatcgcctg attgaccagt acgggccaat
    22321 ctcgcggatt cagattgccg agcaaagcca gcttgccccc gccagcgtaa ccaaaattac
    22381 gcgtcagctt atcgaacgcg ggctgatcaa agaagttgat cagcaggcct ccaccggggg
    22441 ccgccgcgct atctctatcg tcaccgaaac ccgcaatttc cacgcaatcg gcgtacggct
    22501 tggtcgtcac gacgccacca tcaccctgtt tgatctcagc agcaaagtgc tggcagaaga
    22561 acattacccg ctgccggaac gtacccagca gacgctggaa catgccctgc tgaatgccat
    22621 tgctcagttt attgatagct accagcgcaa actgcgcgag ctgatcgcca tttccgttat
    22681 cctgccagga cttgttgacc cggacagcgg caaaattcat tacatgccgc atattcaggt
    22741 agaaaactgg gggctggtag aagcactgga agagcgtttt aaagtgacct gtttcgttgg
    22801 tcacgatatc cgtagtctgg cgctggcgga gcactacttc ggtgcaagtc aggattgcga
    22861 agactccatt ctggtgcgtg tccatcgcgg aaccggggcc gggattatct ctaacgggcg
    22921 catttttatt ggccgcaacg gtaacgtcgg tgaaattggc catattcagg tcgaaccgct
    22981 gggtgaacgc tgccactgcg gcaactttgg ctgcctggaa actatcgctg ccaacgccgc
    23041 tattgaacaa cgggtgttga atctgttaaa gcagggctac cagagccgcg tgccgctgga
    23101 cgactgcacc ataaaaacta tctgcaaagc tgcgaacaaa ggcgatagcc tggcgtcgga
    23161 agtgattgag tatgtcggtc gtcatctggg caaaaccatc gccattgcca tcaacctgtt
    23221 taacccgcaa aaaattgtta ttgccggtga aatcaccgaa gccgataaag tgctgctccc
    23281 tgctattgaa agctgcatta atactcaggc gctgaaggcg tttcgcacta atctgccggt
    23341 ggtacgttct gagctagacc accgctcggc aatcggcgct tttgcgctgg taaaacgcgc
    23401 catgctcaac ggtattttgc tccagcattt gctggaaaat taatgtgctt ttatagtggc
    23461 gcttattgtt gtcaatattc tgggtagtcc atgaccatta aaaatgtaat ttgcgatatc
    23521 gacggcgtgc tgatgcacga taacgtcgcc gtaccgggtg cagcggaatt tttgcacggg
    23581 attatggata aaggcctgcc gctggtgttg ctgaccaact atccttcgca gaccgggcaa
    23641 gatctggcga accgctttgc cactgccggt gtcgatgtac ctgacagcgt gttttatacc
    23701 tctgcgatgg cgaccgccga tttcctgcgt cgccaggaag gcaagaaagc gtatgtggtg
    23761 ggcgaaggcg cactgattca tgaactgtac aaagccggtt tcactattac cgatgtgaac
    23821 cctgattttg tcattgttgg cgaaacgcgt tcctacaact gggacatgat gcataaagcg
    23881 gcctatttcg tcgctaacgg tgcacgcttt atcgccacca acccggatac tcacgggcgc
    23941 ggtttttatc cggcctgtgg cgcgttgtgt gcagggattg agaaaatctc cgggcgcaaa
    24001 ccgttctatg ttggtaagcc gagtccgtgg attatccgcg cggcattaaa caaaatgcag
    24061 gcacattcgg aagaaacggt gattgtcggc gataacctgc gtaccgatat tctggccggc
    24121 ttccaggcag gtctggagac gattctggtg ctttctggtg tttcgtcgct cgacgatatc
    24181 gacagtatgc cgttccgccc cagctggatt tacccatcgg tcgctgaaat cgacgttatc
    24241 tgaatagcaa ccacgtctgc ggacgtggtt ggctctgatg acaaacgcca aactgcctga
    24301 tgcgctacgc ttatcaggcc tacgtagctc ctgcaacata ttgaattttc atgcttttgt
    24361 aggccggata aggcgttcac gccgcatccg gcatgaacaa atcgcacttt gtcagcaatt
    24421 tggcccccgt aaaatataaa cattcaataa aacccgccaa aaattatcga tccattaata
    24481 aatacatcac cgcaccctcc cctttttcac aaatcagcaa tcaccattac gttttttatt
    24541 ttttcaccgc caaatcgctt gcgcacagtg ctgctttgcg gcattttttt aaacaagcaa
    24601 acacaacaag caacaaatac caggttaacg gagaaggtta tgtgttcaat ttttggcgta
    24661 ttcgatatca aaacagacgc agttgagctg cgtaagaaag cactcgagct gtcacgcctg
    24721 atgcgtcatc gtggcccgga ctggtccggt atttatgcca gtgataacgc cattctcgcc
    24781 cacgaacgtc tgtcaattgt tgacgttaac gcgggggcgc aacctctcta caaccaacaa
    24841 aaaacccacg tactggcggt aaacggtgaa atctacaatc accaggcatt gcgcgctgaa
    24901 tacggcgatc gttatcagtt ccagacaggg tctgactgtg aagtgatcct cgcgctgtat
    24961 caggaaaaag ggccggaatt tctcgacgac ttgcagggca tgtttgcctt tgccctgtac
    25021 gacagcgaaa aagatgccta cctgattggt cgcgaccatc tggggatcat cccactgtat
    25081 atgggctatg acgaacatgg tcagctgtat gtggcctcag aaatgaaagc cctcgtgccg
    25141 gtatgccgca cgattaaaga gttcccggcg gggagctatt tgtggagtca ggacggcgaa
    25201 atccgttctt attatcatcg cgactggttc gactacgatg cggtgaaaga taatgtcacc
    25261 gacaaaaacg agctgcgtca ggctctggaa gattccgtta aaagccatct gatgtctgat
    25321 gtgccttacg gtgtgctgct ttctggtggt ctggattcct caattatttc cgctatcacc
    25381 aagaaatacg cagcccgtcg cgtggaagat caggaacgct ctgaagcctg gtggccgcag
    25441 ttacactcct ttgctgtagg tctgccgggt tcaccggatc tgaaagcagc ccaggaagtg
    25501 gcaaaccatc tgggcactgt gcatcatgaa attcacttca ctgtacagga aggtctggac
    25561 gccatccgcg acgtgattta ccacatcgaa acctatgatg tgaccactat tcgcgcttca
    25621 acaccgatgt atttaatgtc gcgtaagatc aaagcgatgg gcattaaaat ggtgctgtcc
    25681 ggtgaaggtt ctgatgaagt gtttggcggt tatctttact tccacaaagc accgaatgcc
    25741 aaagaactgc atgaagagac ggtgcgtaaa ctgctggccc tgcatatgta tgactgcgcg
    25801 cgtgccaaca aagcgatgtc agcctggggc gtggaagcac gcgttccgtt cctcgacaag
    25861 aaattcctcg acgtggcgat gcgcattaac ccgcaggata aaatgtgcgg taacggcaag
    25921 atggaaaaac acatcctgcg tgaatgtttt gagtcatacc tgcccgcaag cgtggcctgg
    25981 cggcagaaag agcagttctc cgatggcgtc ggttacagtt ggatcgacac cctgaaagaa
    26041 gtggcggcgc agcaggtttc tgatcaacag ctggaaactg cccgcttccg cttcccgtac
    26101 aacacaccga cctcaaaaga agcgtatctg taccgggaaa tctttgaaga actgttcccg
    26161 cttccaagcg ccgctgagtg cgtgccgggc ggtccttccg tcgcgtgttc ttcagctaaa
    26221 gcgatcgaat gggatgaagc gttcaaaaaa atggacgatc cgtctggtcg cgcggttggt
    26281 gttcaccagt cggcgtataa gtaagaaatg cgaaacagcc ccggtgaaat ccggggctgt
    26341 ttcagttatt gaggtgcttg ttgattctga tatgcagtaa ggaaatgttc cgggtttgcc
    26401 acatcactgt ttgacaactc atcactgtag gccagtattt catagattaa tttcccacca
    26461 ctattgttaa attccgaatt tcatcctgta tgacaaaaga atggctgtag ggtgggaaaa
    26521 aacacaaagt aaacaataat tgacgaatat agcgccacgc tgttcgcaac ctaaccaaac
    26581 agtcactttc gagcaatttt ccttgaaaaa gaggttgacg ctgcaaggct ctatacgcat
    26641 aatgcgcccc gcaacgccga taaggtatcg cgaaaaaaaa gatggctacg tagctcagtt
    26701 ggttagagca catcactcat aatgatgggg tcacaggttc gaatcccgtc gtagccacca
    26761 tctttttttg cgggagtggc gaaattggta gacgcaccag atttaggttc tggcgccgca
    26821 aggtgtgcga gttcaagtct cgcctcccgc accattcacc agaaagcgtt gtacggatgg
    26881 ggtatcgcca agcggtaagg caccggtttt tgataccggc attccctggt tcgaatccag
    26941 gtaccccagc catcgaagaa acaatctggc tacgtagctc agttggttag agcacatcac
    27001 tcataatgat ggggtcacag gttcgaatcc cgtcgtagcc accaaattct gaatgtgtcg
    27061 aatatgttcg acaaattcaa aaaccaattt gttggggtat cgccaagcgg taaggcaccg
    27121 gattctgatt ccggcattcc gaggttcgaa tcctcgtacc ccagccacat taaaaaagct
    27181 cgcttcggcg agctttttgc ttttctgcgt tcactcaatg tcgaatgcga tgttgacacg
    27241 tcttatcctt caatgtcgga tgcgacgctg ccgcgtctta tccgacctac ggttggcacg
    27301 catccggcaa tgttgtaagg ctacaaccct aacgcatatt tcagcgcctg acgtttcaac
    27361 acgccagcac gctccgccgc catcaaacca agattacgca caaaacgcag cggcggcaga
    27421 ttattactga atccggcata aaacagatcc ataccgctct gcataatgaa gttatccgcc
    27481 atgcgccgca tctggtaacg cttgaggaca ggataactgg cccacgcttc gccgtagctg
    27541 cgggcattca ccagaacatc aatcagggca tcgacatcac gataaccaag attcaccccc
    27601 tgccccgcca gcggatggat ggtatgcgcg gcatcgccca ccagcgcaag ccctggctgc
    27661 acatactgca acgcatggcg acgcgtcagc ggaaacgcac cagcggcaag cggtgtaacg
    27721 taacccagac gcgacgggaa atgcttcgcg atttccgcct ggagctgtgc catattcata
    27781 ttctgcaact ggcgaatacg cgccggagag tcataccaca ccagcgatgc ccagttatca
    27841 aacaacggca gaaacgcacg cggtccgtcc ggagtaaatt gctgccaggt gctatcgccg
    27901 ggatcgttct cgcactggac gctaatcaac atgcacgact gcgcatactg ccatgcatga
    27961 acgccaattc ccgccatctg ccgcacctgc gaatttgcgc cgtcggcacc aatcaccagc
    28021 ttcgagcgaa tcacttcacc gcctttcagc tccagctcct gaagatcatt atctcgatgt
    28081 aatgcaatca gcgagcctgg cacacgtaac gttactttcg gatgcgcttc cagcgcctgc
    28141 cacaacgcct gttgcaggac agtgttttcc accatatagc caagcagcgg tagcttaagt
    28201 tcagcggcgt caaacaccac atgcgccgtt tcccactccc acgtttccag tctgcggtaa
    28261 ggatggcaac gcatagcctg tactgcatcc cagaccccta accctttaag caatgatacc
    28321 gaagccgcgc tgatcgccga gatccgcacg tccggttggc tatcagcgac aaacggtgca
    28381 ggttctgcgt gctcgatcac cgttaccgta aatccgtgct gtgccagccc cagcgccagt
    28441 gcgccgccga ccattcctcc gccgacaatg gcaatttccg ttggttgatt tgtcatggtc
    28501 aatcatcctg ttagcaatat gctttaagtt tacaggattt ttacccccaa gggctgataa
    28561 cgctcatact ggtcacaacg gcagcaaagc attacactat gcgccctgca ttcctggcta
    28621 ctatttcgca agagcaagtc gatgaccaaa aaactccata ttaaaacctg gggctgtcag
    28681 atgaacgagt acgattcatc gaagatggcc gatctgctgg atgccaccca cggctatcaa
    28741 ctgaccgacg tggcggaaga agcggatgtg ctgctgctga acacctgctc aatccgcgag
    28801 aaggctcagg aaaaagtctt ccatcagttg ggtcgctgga aactgttaaa agagaagaat
    28861 ccagacctga ttatcggcgt cggtggctgc gtggcatcgc aagaaggcga gcacattcgc
    28921 cagcgcgccc actatgtcga tattattttt gggccgcaaa cgctgcaccg tctgccggag
    28981 atgatcaact ccgtgcgcgg cgaccgcagc ccggttgtag atatcagctt cccggaaatc
    29041 gagaagtttg accgtctacc ggaaccgcgc gccgaagggc cgaccgcgtt tgtctccatc
    29101 atggaaggct gcaataaata ttgcacctac tgcgtggtgc cttacacccg tggtgaagag
    29161 gtaagccgtc cgtccgacga tattctgttt gagattgccc agcttgcggc tcagggcgtg
    29221 cgtgaagtca acctgctcgg tcagaacgtg aacgcctggc gtggtgaaaa ctacgacggc
    29281 accaccggat cgtttgccga tctgttgcgt ctggttgctg cgatcgacgg gatcgatcgt
    29341 attcgcttta ccaccagcca tccgatcgaa ttcaccgacg atatcatcga agtgtatcgc
    29401 gacacgccgg agctggtgag cttcctgcat ctgccggtac agagcggttc cgatcgcatt
    29461 ctgaacctga tgggacgtac ccatacggcg ctggagtaca aagcgatcat ccgtaaactg
    29521 cgtgcggcgc gtccagatat tcagatcagt tctgatttca tcgttggctt ccctggcgaa
    29581 accaccgaag acttcgagaa aacgatgaag ctgattgccg acgtcaattt cgacatgagc
    29641 tacagcttta tcttctctgc acgtccgggt acaccagccg ccgatatggt tgatgatgtc
    29701 ccggaagaag agaagaagca gcgtctgtat attctgcaag agcgcattaa tcagcaagcg
    29761 atggcgtgga gccgccggat gctcggcacc acccagcgta ttctggtaga aggtacatcg
    29821 cgtaagagca tcatggagct ttccgggcgt acggaaaata accgcgtggt caacttcgaa
    29881 ggcacgccgg atatgatcgg taaattcgtc gatgtagaaa ttaccgacgt ctacccgaac
    29941 tctctgcgcg gtaaagtggt gcgaactgaa gatgaaatgg gtctgcgcgt ggcagaaaca
    30001 ccggaatcgg tgatagcccg tactcgcaaa gaaaacgacc ttggcgtggg ttattatcag
    30061 ccgtaattct caggcccgcc gttccggtgg gccttgtttt ttctctcttt atccccatct
    30121 ttatcgcaat gcttgcgcct ttttcctgtg gcgtgaataa tttccttata ggccggtgaa
    30181 tgttcagctt ccggcggcat acagataaga ggaacggttt gaacatagac actcgcgaaa
    30241 tcaccctgga gccagcagac aacgcgcgtc tgttgagcct gtgcggcccg tttgatgaca
    30301 acatcaagca gctcgaacgc cgtctcggca tcgagatcaa tcgccgcgat aaccacttta
    30361 aactgaccgg ccgtccgatt tgcgtcaccg ctgcggcaga cattctgcgt agcctgtatg
    30421 tcgatactgc cccgatgcgc ggtcagattc aggatattga accggaacag atccaccttg
    30481 cgattaaaga agcgcgggta ttggagcaaa gtgcggagag cgtgccggag tacggcaaag
    30541 cggtcaatat caaaaccaaa cgcggcgtaa ttaagccgcg cacgccaaac caggcgcagt
    30601 acatcgccaa tattctcgac catgacatta ccttcggcgt tggcccggcg ggtacgggta
    30661 aaacctacct ggcagtggct gcggcagttg atgccctgga gcgtcaggaa attcgccgta
    30721 ttctgctgac tcgtccggcg gtcgaagccg gtgagaaact gggcttcctg cctggcgatt
    30781 taagccagaa agtagacccg tatctgcgcc cactgtacga cgcgctgttt gaaatgctgg
    30841 gctttgagaa agtcgagaaa ctgattgagc gcaacgttat tgaagtcgcg ccgctggcct
    30901 atatgcgtgg tcgtacgctg aatgacgcct ttatcattct cgatgagagc cagaacacca
    30961 ccatcgaaca gatgaagatg ttcctgaccc gtatcggttt taactcaaaa gcggttatca
    31021 ccggcgacgt cacacagatc gacctgccgc gtaatactaa atcaggctta cgtcatgcca
    31081 tcgaagtgct ggccgatgtc gaagagatca gctttaactt cttccacagc gaagacgtgg
    31141 ttcgtcaccc ggtggtggcg cgtatcgtta acgcctatga agcctgggaa gaagccgaac
    31201 aaaaacgtaa agcggcgctg gcggcagaac gcaagcgcga agaacaggaa caaaaatgag
    31261 tcaggtgatc ctcgatttac aactggcatg tgaagataac tccggattgc cggaagagag
    31321 ccagtttcaa acatggctga atgcggtgat cccgcagttt caggaagagt cggaagtgac
    31381 gattcgcgtg gtcgataccg ccgaaagcca cagtctcaat ctgacctatc gcggtaagga
    31441 taagccgacc aacgtgctct ccttcccgtt tgaagtgccg cctggcatgg aaatgtcgct
    31501 actgggcgat ctggttatct gccgtcaggt ggttgagaag gaagctcagg agcaaggcaa
    31561 accactggag gcgcactggg cgcatatggt ggtgcacggc agtctgcatt tgttaggtta
    31621 cgatcacatc gaagatgacg aagcagaaga aatggaagcc ctcgaaacag agattatgct
    31681 tgctctgggc tatgaggatc cgtacattgc cgagaaagaa taatttgcca gccatttgac
    31741 tggcagacgt ccccgccgtt ggtgattaac gacggcgttt tgattaacta acatgagaac
    31801 ccatacgacg ccatgagcga cgacaattca cacagtagtg acacgataag caacaagaag
    31861 ggatttttct ccctgttact cagccaactt ttccacggtg aaccgaaaaa ccgtgacgaa
    31921 ctgctggcgc tgatccgtga ttccgggcag aacgacctta tcgacgaaga tacgcgcgat
    31981 atgctcgaag gggtgatgga catcgcagac caacgcgtcc gcgacatcat gatcccccgc
    32041 tcccagatga ttaccctgaa acgcaaccag acgctggacg aatgccttga tgtcatcatc
    32101 gagtccgccc actcacgttt cccggtgatt agcgaagaca aagatcacat tgaagggatt
    32161 ctgatggcga aagacttgct gccgtttatg cgcagcgatg ctgaagcctt cagcatggac
    32221 aaagtgttac gtcaggcagt tgtcgttcct gaaagtaagc gcgtagaccg gatgctgaaa
    32281 gagtttcgct ctcagcgtta ccacatggcg atcgttattg acgaattcgg tggggtttcc
    32341 ggtctggtaa ccattgaaga catcctggaa ctgattgttg gtgagattga agacgaatat
    32401 gacgaagaag atgatatcga cttccgtcag ctgagtcgtc atacctggac cgtgcgcgca
    32461 ctggcttcca ttgaagactt caacgaagcg ttcggcaccc actttagcga tgaagaagtc
    32521 gacactatcg gtggtctggt gatgcaggca tttgggcatc ttccggcacg tggcgaaacc
    32581 atcgacatcg acggttacca gttcaaagtg gcgatggccg acagtcggcg tattattcag
    32641 gttcatgtca aaatcccgga tgactcaccc cagccgaagc tggatgaata aaaccgaaac
    32701 tggatagata actacatggc ttttgcctca ttaattgaac gccagcgcat tcgcctgctg
    32761 ctggcgttat tattcggtgc ctgcggaacg ctggccttct ctccttacga cgtctggcct
    32821 gcggcgatta tttcgctgat ggggcttcag gcgctgacct ttaaccgccg tccactccag
    32881 tctgccgcta ttggcttttg ctggggattt ggcctgtttg gcagcggtat taactgggtc
    32941 tatgtcagta tcgcgacctt tggcggaatg cctggcccgg ttaacatctt cctggtggtg
    33001 ctgctggcgg cgtatttgtc gctgtatacc ggactgtttg ctggcgtgtt gtcgcgtctg
    33061 tggtcgaaaa ccacctggct gcgcgtggcg attgtcgctc ctgctctctg gcaagtgacc
    33121 gagtttctgc gcggttgggt gctgactggc ttcccgtggt tacagttcgg ctatagccag
    33181 attgatggcc cgttaaaagg gctggcaccg ctaatgggcg tggaagccat taacttcctg
    33241 cttatgatgg ttagcggcct gctggcactg gcgttagtca aacgcaactg gcgtccgctg
    33301 gtggtggccg tcgtgctgtt tgcccttccc ttcccgctgc gttacatcca gtggtttacc
    33361 ccacaaccgg agaaaaccat tcaggtttcg atggttcagg gcgatattcc gcaatcgctg
    33421 aaatgggacg aaggccagct tcttaatacg ctgaagattt actacaacgc aacggcaccg
    33481 ctgatgggca aatcatcgtt gattatctgg ccggagtcgg cgataaccga tctggaaatt
    33541 aatcagcaac cgttcctcaa agcactggac ggtgagttgc gtgataaagg gagtacgctg
    33601 gtgaccggga ttgttgacgc gcgtcttaat aagcagaacc gttacgatac ctacaacacc
    33661 atcatcacac tgggtaaagg tgcaccgtac agctacgaat cagccgatcg ctataacaaa
    33721 aaccatctgg tgccgtttgg cgagtttgtc ccgctggagt cgattctgcg tccgttagca
    33781 ccgttctttg atctgccgat gtcgtcgttc agccgtgggc catatatcca gccgccgctg
    33841 tcggcaaatg gtattgagct tactgcggct atttgctacg agatcattct cggcgagcaa
    33901 gtgcgcgata acttccgccc ggataccgac tatctgctga ctatctccaa cgatgcgtgg
    33961 tttggtaagt ctattggtcc atggcagcac ttccagatgg cgcgaatgcg tgcgctggag
    34021 ctggcgcgcc cattgctgcg tagtaccaac aacggcatta cggcggtgat tggtccgcag
    34081 ggtgagattc aggcgatgat ccctcagttc acccgcgaag tgttgaccac taacgtgacg
    34141 ccgaccaccg gactcacacc atacgcacgt accggcaact ggccgctgtg ggtgctgacg
    34201 gcactgtttg gttttgccgc tgtgctgatg agtctgcgtc aacgacgtaa ataatccctt
    34261 cttatcgtgc cggaatacat cccgtatttc ggcacgctca ccggattcgg catcagtgcg
    34321 ttatccggat aacaaaattc ccctatccaa ctttggcaca tctattgctt tgttatacaa
    34381 ggcaaagctt gaaccagcat cagtacaacc cggttgcacc acagcaatcc accggtagca
    34441 ccaaaagagt gcattttaag aattttgctt cggaatggtg cggcgcacct cccaaatata
    34501 aacaataaaa cagcaatatc tttacattta gccaaactaa atgttaccta acaggcacaa
    34561 cactgcacaa taaagttgca gacgataaca acacaaacac tcacaacggg tatccatgcg
    34621 ttcttaacgc agaagataaa ggagttggat atgcaattac gtaaacctgc cacagcaatc
    34681 ctcgccctgg cgctttccgc aggactggca caggcagatg acgccgcccc ggcagcaggc
    34741 agtacgctgg acaaaatcgc caaaaacggt gtgattgtcg tcggtcaccg tgaatcttca
    34801 gtacctttct cttattacga caatcagcaa aaagtggtgg gttactcgca ggattactcc
    34861 aacgccattg ttgaagcagt gaaaaagaaa ctcaacaaac cggacttgca ggtgaaactg
    34921 attccgatca cctcacaaaa ccgtattcca ctgctgcaaa acggcacttt cgattttgaa
    34981 tgtggttcca ccaccaataa cgtcgaacgc caaaaacagg cggctttctc tgacactatt
    35041 ttcgtggtcg gtacgcgcct gctgaccaaa aagggcggcg atatcaaaga ttttgccgac
    35101 ctgaaaggca aagccgtagt cgtcacttcc ggcactacct ctgaagtttt gctcaacaaa
    35161 ctgaatgaag agcaaaaaat gaatatgcgc atcatcagcg ccaaagatca cggtgactct
    35221 ttccgcaccc tggaaagcgg tcgtgccgtt gcctttatga tggatgatgc cctgctggcc
    35281 ggtgaacgtg cgaaagcgaa gaaaccagac aactgggata tcgtcggcaa gccgcagtct
    35341 caggaagcct acggttgcat gttgcgtaaa gacgatccgc agttcaaaaa gctgatggat
    35401 gacactatcg ctcaggtgca gacctccggt gaagcggaaa aatggtttga taaatggttc
    35461 aaaaatccca ttccgccgaa aaacctgaac atgaatttcg aactgtcaga cgaaatgaaa
    35521 gcactgttca aagaaccgaa tgacaaagca ctgaactaat tacaagaacc aggggcggaa
    35581 attctagccc tctcgattgt tacgtagcac ggacagacta tacgcctgat ggtcgttccc
    35641 catcgggcct gaaaaccgca atacgctggg taacaatctt cgagggtagc agttaacgct
    35701 gctaccctct tttttctgga gtagatttat gtctatagac tggaactggg gtattttttt
    35761 acaacaagcc ccgttcggca acaccaccta tcttggttgg atctggagtg gttttcaggt
    35821 gacgatcgct ttgtcgatct gcgcctggat tatcgctttc ctcgtcggtt catttttcgg
    35881 cattttacgt accgttccaa accgttttct ctccggtctg ggtacgttgt atgtcgaact
    35941 gttccgcaac gtgccgttga ttgtgcaatt ctttacctgg tatctggtga tcccggagtt
    36001 gctgccagag aaaatcggca tgtggtttaa ggccgagctg gacccaaata ttcagttttt
    36061 cctctcctcc atgctctgtc tggggctgtt taccgccgcc cgtgtttgcg aacaggtacg
    36121 cgccgccatt cagtcgctgc cgcgcgggca aaagaatgcc gcgctggcga tggggctgac
    36181 gctgccgcag gcttaccgct atgtactgtt gcctaatgct tatcgcgtta tcgtcccgcc
    36241 gatgacctcc gagatgatga acctggtgaa aaactccgcc atcgcctcga ctatcggtct
    36301 ggtagatatg gcagcgcagg cgggtaaatt gctcgattac tcggcccacg cctgggagtc
    36361 gtttaccgcc attacgctgg cgtatgtgtt gattaacgcg gttatcatgc tggtaatgac
    36421 gctggttgaa cgtaaagttc gcctgcctgg caacatgggg ggcaaataat gtacgagttt
    36481 gactggagtt ccattgtccc ttccctgcca tatctgctcg acgggctggt gatcacgctg
    36541 aaaatcaccg tcacggcagt cgtgattggt attttgtggg gcaccatgct ggcggtgatg
    36601 cgcttgtcca gctttgcgcc cgtagcgtgg tttgccaaag cctacgttaa cgtattccgc
    36661 tcgattcctt tagttatggt tttgctgtgg ttttacctga tcgtgccggg ttttctgcaa
    36721 aacgtgctgg gattatcgcc aaaaaatgat atccgtttaa tttcggcgat ggtggcgttt
    36781 tcgatgtttg aagcggccta ctattcagag attatccgtg ctggtattca aagtatttct
    36841 cgtggtcagt ctagcgccgc gctggcgttg ggaatgactc actggcagtc gatgaaactg
    36901 attattctgc cgcaggcgtt ccgcgcgatg gtgccgctgc tgctcactca gggcatcgta
    36961 ctgttccagg atacctcgct ggtgtatgtg ttatctctgg ccgatttctt ccgtaccgcc
    37021 tcaaccattg gtgagcgtga tggtacgcag gttgagatga tcctgtttgc cggatttgtt
    37081 tatttcgtta ttagccttag cgcgtcgttg ttggtcagct acttgaaaag aaggacagca
    37141 taatgattac cctgaaaaat gtttcaaaat ggtatggtca ctttcaggtg ctgaccgact
    37201 gctcaaccga agtgaaaaaa ggcgaagtgg tggtggtttg cggcccgtct ggttccggca
    37261 aatcaacgct gattaaaacc gtcaacggcc tcgaaccggt gcagcaaggt gaaatcaccg
    37321 tcgatggtat cgtggttaac gataagaaaa ccgatctggc aaagctgcgt tctcgcgtcg
    37381 ggatggtatt ccaacatttc gagctgttcc ctcatctgtc gattatcgaa aacctgaccc
    37441 tggcgcaggt gaaagtgctt aaacgcgata aagcaccggc tcgtgaaaaa gcattgaaat
    37501 tgctggagcg cgtgggactt tccgcacatg ccaataaatt tccggcgcag ctttccggcg
    37561 gccagcagca gcgtgtggca attgctcgcg cattgtgtat ggatcctatt gcgatgctgt
    37621 tcgacgaacc cacatcggca ctagacccgg agatgatcaa cgaagtactg gacgtgatgg
    37681 tggaactggc gaacgagggg atgaccatga tggtggtgac ccacgaaatg ggctttgccc
    37741 gtaaagtggc gaatcgggtg atctttatgg atgagggtaa aattgttgaa gactcgccga
    37801 aagatgcgtt cttcgatgat ccgaaatcgg accgcgcaaa agacttcctc gcgaaaatcc
    37861 tgcattaatc actctggcgc gcatacccaa gtgcgcgcca tctcacgctt tatcgtctcg
    37921 cccctcgtat ctgttacatc ctggcgttac ccttgtcgca aagaagcaca caacaaggag
    37981 caacaatggc actgccaatt ctgttagatt gcgacccagg tcatgatgac gctatcgcaa
    38041 tagttctcgc cctcgcctca ccagagcttg atgtcaaagc aattacgtct tccgccggaa
    38101 accagacacc agaaaaaacc ttacgcaatg ttctgcgtat gctgaccttg cttaatcgca
    38161 ccgatattcc ggtggcaggc ggcgcggtaa aaccgttaat gcgtgagttg attatcgcgg
    38221 acaatgtgca cggcgaaagc ggtctcgacg gcccggcatt accggaaccg acgtttgcac
    38281 cgcaaaagtg tacggcggtg gagctgatgg cgaaaacgct acgtgaaagt gcggaacctg
    38341 tcaccattgt ttctaccgga ccgcaaacta acgttgcttt gctgctcaat agccatccgg
    38401 aactgcatag caaaattgcc cgtatcgtga ttatgggcgg cgcaatgggg ctgggtaact
    38461 ggacgcctgc ggctgaattt aatatttacg ttgacccaga agcggcagaa attgtattcc
    38521 agtcagggat cccggtggtg atggccggtc tggatgttac tcacaaagca caaattcacg
    38581 gtgaggacac cgagcgtttc cgcgcgattg gcaaccctgt ttcaaccatt gttgccgaac
    38641 tgctggattt cttcctcgaa tatcataaag acgaaaaatg gggctttgtc ggcgcaccgc
    38701 tgcatgaccc atgcaccatc gcctggctgt tgaaacccga gttgtttacc actgttgagc
    38761 gctgggttgg cgtggaaaca caggggaaat atacccaggg gatgacggtt gttgattatt
    38821 attatctgac tggcaataaa ccgaatgcca ctgtaatggt cgatgttgat cgtcagggct
    38881 ttgttgattt actggctgat cgtctgaaat tttacgctta acacacgtca ttgattaaga
    38941 tcaccacgcg ttacctgggt ggtcttaatc tgtttatctc tttttctttc aagatataaa
    39001 tgcaatggat aatgcagaac tcgccattgg tatcgatctc ggtactacca atagtttaat
    39061 tgccgtctgg aaagacggtg ccgcgcaatt aattccaaat aagttcggtg aatatttaac
    39121 accatccata attagcatgg atgaaaataa tcatatttta gtcggaaaac cggctgtatc
    39181 acggcgtact tcgcatccgg ataaaacggc agcgttattt aaacgtgcaa tgggcagtaa
    39241 taccaactgg cggttaggca gcgacacatt taacgcgcca gaactgtcct ctttggtatt
    39301 acgctcatta aaagaagatg ccgaagaatt tctgcaacgt ccgattaaag atgtggtgat
    39361 ctctgttccg gcttatttca gcgatgaaca acgcaagcat acccgtttag cagcggagtt
    39421 agccggttta aatgcggtac gcttaattaa tgaacccaca gcagctgcga tggcgtatgg
    39481 cctgcatacc caacaaaata cccgttcgct ggtgtttgat ctcggtggcg gcacgtttga
    39541 cgttacggtg cttgagtacg ccacgccggt gattgaagtt cacacctccg ctggcgacaa
    39601 cttcctcggt ggcgaagatt ttacccatat gctggtcgat gaggttttaa aacgcgcgga
    39661 tgtcgccagg accacgctta acgagagtga actggcagcc ttgtatgcct gtgtggaagc
    39721 ggcaaaatgt agcaatcaat cgccaatgca cattcgctgg cagtaccagg atgaaacgcg
    39781 ggaatgcgaa ttttacgaga acgaactgga agatttgtgg ttgccgctgc tcaatcgctt
    39841 gcgagtgccg attgaacagg cgttgcgcga tgcacgtctg aagccgagtc aaatcgacag
    39901 tctggtgctg gttggcggcg cgtcacaaat gccgctggtg cagcgaatcg ccgtgcgtct
    39961 gtttggcaaa ttaccgtatc aaagttacga tccgagcacc attgtcgcgc tgggcgcagc
    40021 aattcaggct gcctgccgct tacgcagtga agatattgaa gaagtgatcc tcactgatat
    40081 ttgcccttac tcgttgggcg ttgaggttaa ccgtcagggc gtttccggca ttttctcgcc
    40141 gattattgaa cgaaacacca ctgtgcccgt gtcgcgtgta gaaacttatt caaccatgca
    40201 cccggaacag gattcaatta cggttaacgt ctatcaggga gaaaaccaca aagttaaaaa
    40261 caacattctg gtggaatcct tcgatgtgcc gttgaagaaa accggggctt atcagtcgat
    40321 tgatattcgc tttagttatg atatcaacgg tttgcttgaa gttgacgtgc ttctggaaga
    40381 cggcagcgtt aagtccagag tgattaacca cagcccggta acattgagcg cgcagcagat
    40441 tgaagagagt cggacgcggt tatccgcatt gaaaatttat ccgcgcgata tgctcatcaa
    40501 tcgtaccttt aaagccaaac tggaagagtt gtgggcgcgg gcgctgggtg acgagcgaga
    40561 agagattggc cgggtgatca ccgattttga tgcggcgctg cagtcaaacg atatggcccg
    40621 cgtcgatgag gtcaggcggc gggcgagcgt ttatttagcc attgagatcc cataatcacg
    40681 gctgccggat gcggcgtaaa tgctgcatcc ggcatcggcg caaaaaacac atcgtcaact
    40741 aaacaacatc gcgattttgc cgagagcata acctataacc agcaccgtta aactgatttt
    40801 gataatgctg gtaatgctct gtggtttttc accctcatca tttttttctt catggttttc
    40861 aggtgttggc tcatcgacat cttcatcctc cggctcgagt tttactttcg tcaacagcat
    40921 ccgggtgata atggcctcta atggatgagc atctccgcct tccagccttg tattaagacg
    40981 ggccagcatc tcttgtttcg gaaacgcttc aagtactacc tgttccgcta cccgatacaa
    41041 atccagtaac ggtgaatctt gctctgtctc gctaccgact ctcgatttcc accacgccca
    41101 tgctttgcgt ttgaagaata acgactcaat aaacgattga cctaacggcg atagttttcc
    41161 gctatgtaaa atttccgccc ataacagacg cgtccgggcg ctctcaccag gaacatattt
    41221 caacggttta ccttgcgcat ctaccatgct ccgcgcttcg ataaccatca ctaataacgc
    41281 gaaataatcc gggcaatgtt gtgcgctcca ctgcaacaac aaattatcgg cttgtgtaga
    41341 tgggtaactt ccccagtacg cgcagagatc ggcaagcagg ctttccccct cgccgcagta
    41401 caaacgttgt gcacactggt aataccgggc atcttcgctt tctggttctt ccatttgcca
    41461 ctgtttggct acaggaatga gttccgccat acctgtttgc accgagctgt accagcgcaa
    41521 cagtttgcga tgtaactttg catcatcagg aatgaaccac ggaccttcca tcgccaaaaa
    41581 agcagtgaca tagtcaggat ggtaatggca gcaacgttcc agcgcgaagt aatattcaac
    41641 agtctggttc tgcaacgcaa cgggatattc cagaaggcca aggaaatcga aacaatcacc
    41701 agcctgaatg gcctcaagaa aagcctccct ttcttcctca tccattccta cgctgtcatt
    41761 aaacgaatgc cagttcaaac gctctgccag caaagaagca caatttaatg aaagatatcg
    41821 cgcttctata gcgatggcac acagcggcca gcgtaattga tcgacatcct ccatgttcca
    41881 ggtatttaat tgctgaataa atttctgcca ggcggagggc tgaaaacgat cactttctga
    41941 atccagtaat gccctgaata cacgtagtat ttcatgctca gtggcatcat ctttttcttc
    42001 atcatctgct tcctcgacag gatttaccgc cagtcgcagc gcctcttcat aggcctggcg
    42061 taatgcttta aacccctgcg gatcggtttc gggatgacat aacggtaagc gagcaagata
    42121 agcctggcgg ataatgtcta tttgcgtcgt gctttcaatt tcgaggattt gccaacatgt
    42181 tttcataggt cgctcacatc cttaagcgtt accgggcatt caggctggac agttaatggc
    42241 tgccagggaa gcgtggcata gaaacgatcg cttcccgctg ccagttcttc gaacatacga
    42301 ctcccggcgc tatcgtattt gtacagggtt agcgcctcgc gtagcgaagt gttgtcttcg
    42361 cactgagaag attgccagta gagacgccct agtgaatagg cagaaaaata gtgcatccag
    42421 ctgtgatagt aatggtgagc tcgtacatag acgcgcgatt gcagccataa actctcgtct
    42481 tccgtcagcc acttgttgag cacaccgacc cgtgacagaa accccattcg aacataatcc
    42541 caagctttga ttcccccttc tccacagcac atagcggttt gggcaacgaa ttgggtataa
    42601 atcaaccctc tctccgagcc accagcaatt aatgctttcc actcttcagg ggaagagcga
    42661 tgccatgtca gatataagcc agctaaatca ttagcatgac catcatcagt catctgataa
    42721 atagtctgaa ttaaatcatc acgattattt attccccagg atgatgagag atcgaattct
    42781 tttatcccgg tatacatttt tggcgagtca tgcgcgggtt tgcaaccctg attaagtatt
    42841 tccatgggcg cagaaagcgc aaaaagaaga tattgttctt ccttat
//
LOCUS       DAZFKF010000034        42876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFKF010000034 DAZFKF010000000
VERSION     DAZFKF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.034"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            44..946
                     /gene="ybdO"
                     /locus_tag="ACHKA1_002254"
     CDS             44..946
                     /gene="ybdO"
                     /locus_tag="ACHKA1_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5679901.1"
                     /translation="MTNLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     HGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNECF"
     gene            1093..2313
                     /gene="ybdN"
                     /locus_tag="ACHKA1_002255"
     CDS             1093..2313
                     /gene="ybdN"
                     /locus_tag="ACHKA1_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO5679902.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2286..2915
                     /gene="ybdM"
                     /locus_tag="ACHKA1_002256"
     CDS             2286..2915
                     /gene="ybdM"
                     /locus_tag="ACHKA1_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO5679903.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2916..4076)
                     /gene="ybdL"
                     /locus_tag="ACHKA1_002257"
     CDS             complement(2916..4076)
                     /gene="ybdL"
                     /locus_tag="ACHKA1_002257"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO5679904.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4185..5273
                     /gene="hcxA"
                     /locus_tag="ACHKA1_002258"
     CDS             4185..5273
                     /gene="hcxA"
                     /locus_tag="ACHKA1_002258"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO5679905.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNNAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5283..5480)
                     /gene="ybdD"
                     /locus_tag="ACHKA1_002259"
     CDS             complement(5283..5480)
                     /gene="ybdD"
                     /locus_tag="ACHKA1_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO5679906.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5662..7766)
                     /gene="cstA"
                     /locus_tag="ACHKA1_002260"
                     /pseudo
     CDS             complement(5662..7766)
                     /gene="cstA"
                     /locus_tag="ACHKA1_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
     gene            complement(7947..8360)
                     /gene="entH"
                     /locus_tag="ACHKA1_002261"
     CDS             complement(7947..8360)
                     /gene="entH"
                     /locus_tag="ACHKA1_002261"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO5679907.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8363..9109)
                     /gene="entA"
                     /locus_tag="ACHKA1_002262"
     CDS             complement(8363..9109)
                     /gene="entA"
                     /locus_tag="ACHKA1_002262"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO5679908.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9109..9966)
                     /gene="entB"
                     /locus_tag="ACHKA1_002263"
     CDS             complement(9109..9966)
                     /gene="entB"
                     /locus_tag="ACHKA1_002263"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO5679909.1"
                     /translation="MAIPKLQAYALPEAHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9980..11590)
                     /gene="entE"
                     /locus_tag="ACHKA1_002264"
     CDS             complement(9980..11590)
                     /gene="entE"
                     /locus_tag="ACHKA1_002264"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO5679910.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAANNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFIAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11600..12775)
                     /gene="entC"
                     /locus_tag="ACHKA1_002265"
     CDS             complement(11600..12775)
                     /gene="entC"
                     /locus_tag="ACHKA1_002265"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO5679911.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQLLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12964..13920
                     /gene="fepB"
                     /locus_tag="ACHKA1_002266"
     CDS             12964..13920
                     /gene="fepB"
                     /locus_tag="ACHKA1_002266"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO5679912.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGIHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13924..15174)
                     /gene="entS"
                     /locus_tag="ACHKA1_002267"
     CDS             complement(13924..15174)
                     /gene="entS"
                     /locus_tag="ACHKA1_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO5679913.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            15285..16289
                     /gene="fepD"
                     /locus_tag="ACHKA1_002268"
     CDS             15285..16289
                     /gene="fepD"
                     /locus_tag="ACHKA1_002268"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO5679914.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16286..17278
                     /gene="fepG"
                     /locus_tag="ACHKA1_002269"
     CDS             16286..17278
                     /gene="fepG"
                     /locus_tag="ACHKA1_002269"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO5679915.1"
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17275..18090
                     /gene="fepC"
                     /locus_tag="ACHKA1_002270"
     CDS             17275..18090
                     /gene="fepC"
                     /locus_tag="ACHKA1_002270"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO5679916.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(18087..19220)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKA1_002271"
     CDS             complement(18087..19220)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKA1_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO5679917.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(19436..23317)
                     /gene="entF"
                     /locus_tag="ACHKA1_002272"
     CDS             complement(19436..23317)
                     /gene="entF"
                     /locus_tag="ACHKA1_002272"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO5679918.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLAAQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHSERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLREHGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRAP
                     KAGSETIIAAAFSSLLGCDVQDVDADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPLWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(23314..23532)
                     /gene="ybdZ"
                     /locus_tag="ACHKA1_002273"
     CDS             complement(23314..23532)
                     /gene="ybdZ"
                     /locus_tag="ACHKA1_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO5679919.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(23535..24737)
                     /gene="fes"
                     /locus_tag="ACHKA1_002274"
     CDS             complement(23535..24737)
                     /gene="fes"
                     /locus_tag="ACHKA1_002274"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO5679920.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLEAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24980..27220
                     /gene="fepA"
                     /locus_tag="ACHKA1_002275"
     CDS             24980..27220
                     /gene="fepA"
                     /locus_tag="ACHKA1_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO5679921.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAAGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            27395..28015
                     /gene="entD"
                     /locus_tag="ACHKA1_002276"
     CDS             27395..28015
                     /gene="entD"
                     /locus_tag="ACHKA1_002276"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO5679922.1"
                     /translation="MKTTHTSLPFAEHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(28137..28289)
                     /gene="hokE"
                     /locus_tag="ACHKA1_002277"
     CDS             complement(28137..28289)
                     /gene="hokE"
                     /locus_tag="ACHKA1_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO5679923.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            28258..28526
                     /locus_tag="ACHKA1_002278"
                     /pseudo
     CDS             28258..28526
                     /locus_tag="ACHKA1_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28741..29859
                     /gene="ybdK"
                     /locus_tag="ACHKA1_002279"
     CDS             28741..29859
                     /gene="ybdK"
                     /locus_tag="ACHKA1_002279"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO5679924.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29925..30173
                     /gene="ybdJ"
                     /locus_tag="ACHKA1_002280"
     CDS             29925..30173
                     /gene="ybdJ"
                     /locus_tag="ACHKA1_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO5679925.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            30238..30606
                     /gene="ybdF"
                     /locus_tag="ACHKA1_002281"
     CDS             30238..30606
                     /gene="ybdF"
                     /locus_tag="ACHKA1_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO5679926.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30700..31353
                     /gene="nfsB"
                     /locus_tag="ACHKA1_002282"
     CDS             30700..31353
                     /gene="nfsB"
                     /locus_tag="ACHKA1_002282"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO5679927.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31611..32858
                     /gene="ybdG"
                     /locus_tag="ACHKA1_002283"
     CDS             31611..32858
                     /gene="ybdG"
                     /locus_tag="ACHKA1_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO5679928.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(32926..34302)
                     /gene="pheP"
                     /locus_tag="ACHKA1_002284"
     CDS             complement(32926..34302)
                     /gene="pheP"
                     /locus_tag="ACHKA1_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO5679929.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTIDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(34404..37547)
                     /gene="cusA"
                     /locus_tag="ACHKA1_002285"
     CDS             complement(34404..37547)
                     /gene="cusA"
                     /locus_tag="ACHKA1_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO5679930.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKIQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDTTLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(37559..38782)
                     /gene="cusB"
                     /locus_tag="ACHKA1_002286"
     CDS             complement(37559..38782)
                     /gene="cusB"
                     /locus_tag="ACHKA1_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO5679931.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFDRAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDSATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(38798..39130)
                     /gene="cusF"
                     /locus_tag="ACHKA1_002287"
     CDS             complement(38798..39130)
                     /gene="cusF"
                     /locus_tag="ACHKA1_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO5679932.1"
                     /translation="MKKALQVAMFSLFTVIGFNVQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(39154..40536)
                     /gene="cusC"
                     /locus_tag="ACHKA1_002288"
     CDS             complement(39154..40536)
                     /gene="cusC"
                     /locus_tag="ACHKA1_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO5679933.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALENNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNGGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            40693..41376
                     /gene="cusR"
                     /locus_tag="ACHKA1_002289"
     CDS             40693..41376
                     /gene="cusR"
                     /locus_tag="ACHKA1_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO5679934.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            41366..42814
                     /gene="cusS"
                     /locus_tag="ACHKA1_002290"
     CDS             41366..42814
                     /gene="cusS"
                     /locus_tag="ACHKA1_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO5679935.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLVMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEIGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVDHQVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIVLPERG"
BASE COUNT        10321 a        11821 c        10883 g         9851 t
ORIGIN      
        1 taagtgaata tatcatggaa gaaaaatata accggagtag tgtatgacca atctctacga
       61 cttgaaaaag ttcgacttaa atcttcttgt catatttgag tgtatttacc agcatttaag
      121 cattagcaaa gctgctgaat cactatatat caccccatca gccgtgagcc agtcgttaca
      181 acgcttacgt gcgcaattta atgacccttt attcattcgc tcaggaaaag gtatcgcgcc
      241 gacgacaaca gggttaaatc tgcatcacca tcttgagaaa aatctcagag gccttgaaca
      301 aacaattaat atagtcaata agtccgaact aaagaaaaac tttattattc acggccctca
      361 gctcatttct tgctctaata atagtatgct gatccgctgc ctacggcaag attcctctgt
      421 cgagattgag tgccatgata tcctcatgtc agcagagaat gctgaagaac tgttggttca
      481 ccgcaaagcc gatctggtga tcacccaaat gccagtgatc agccgttccg tcatttgcat
      541 gccattgcat acgattcgca atactctaat ttgtagtaat aggcatccac gaattactga
      601 caatagcact tatgaacaaa ttatggcaga agagttcacc caattaatat ctaaatcagc
      661 aggggttgat gatattcaaa tggaaataga cgagagattt atgaatcgca aaatttcatt
      721 tcgtggttcc tctttgctga caatcattaa tagcattgca gttacagatt tactgggaat
      781 agtcccctat gaactttata actcccaccg tgactttctt aatttaaaag agataaaact
      841 agaacatccg ttaccttcaa taaagcttta catttcctat aataaatctt ctttaaacaa
      901 tctggtgttc tcccgattta ttgaccgtct aaatgagtgt ttttgatata taacctcaga
      961 cagctactat aactttcacc tatttattca cagcgcgaat gaaaaataaa acactctgct
     1021 tttcagtgtt tactttataa ttaacagtct tatctcactg atcaccctgt aacgttcaga
     1081 gagcgtcttc agatgtctat ttataaaatt cctcttccgc tcaatattct ggaagctgca
     1141 cgagagcgta ttacctggac actcaacacc ctaccccgcg tttgtgtctc attttctggc
     1201 ggcaaagact ctggtttaat gctgcatctg acagccgaac ttgcccgaca aatgggcaaa
     1261 aaaatctgcg ttttgtttat cgactgggag gcgcaattct cttgcaccat taactatgtt
     1321 cagtccctgc gcgagttgta caccgatgtc atcgaagagt tttactgggt tgcgctcccg
     1381 cttacgacgc aaaattccct ttcacaatac caacccgaat ggcagtgctg ggaacctgat
     1441 gtcgaatggg tgcgtcagcc cccgcaagat gcgataaccg atcctgactt tttctgcttt
     1501 taccagccag gcatgacctt cgaacaattt gtacgtgaat ttgccgaatg gttttcacaa
     1561 aaacgtccgg cggcgatgat gatcggcatc cgtgcggatg agtcctacaa ccgttttgtc
     1621 gccatcgcca gtttaaataa acaacgtttt gccgacgata aaccctggac tacggccgca
     1681 ccaggcggtc atagctggta catttacccc atttacgact ggaaagtggc tgatatctgg
     1741 acctggtatg caaatcatca aagtctctgc aacccactgt ataacctaat gtatcaggca
     1801 ggcgttcctc tgcgtcatat gcgaatttgc gaaccttttg gcccggagca acgacaagga
     1861 ttatggctct atcacgttat cgaaccggat cgctgggctg ctatgtgcgc acgagtcagc
     1921 ggggtaaaaa gtggcggcat ttacgccgga catgacaatc atttctatgg gcatcggaaa
     1981 atcctcaagc cagaacattt agactggcaa gaatatgcat tattgctgct caatagcatg
     2041 ccggaaaaaa cagctgagca ttaccgcaat aaaatcgcca tttatttgca ctggtatcag
     2101 aaaaaaggca tcgaggttcc acaaacccag caaggggaca ttggcgcgaa agatatcccc
     2161 tcctggcggc ggatatgcaa agttttactc aataacgatt actggtgtcg ggcattatca
     2221 tttagtccta cgaaagcgaa gaactatcag cgttataacg aacggataaa aggaaaacgt
     2281 caggaatggg ggatactatg caacaacgat taacgcagga tttaacgcaa tttctcgcca
     2341 gcttgccgga agatgatcgc atcaacgcga ttaatgaaat ccgcatggcg atccatcagg
     2401 ttagcccctt tcgtgaagag ccagtggatt gcgttctgtg ggttaaaaac agccaactta
     2461 tgcctaatga ttacaaccca aacaacgtgg caccacccga aaaaaaactg ctgaaaaaat
     2521 ccattgaaat agatggtttt acccaaccca ttgtggtcac gcatacggat aaaaatgccc
     2581 tggaaatcgt ggatggtttt caccgccatg aaattggtaa aggctcatca tcattaaaat
     2641 tacgcttaaa aggttatttg ccggtgacct gtctggaagg tacgcgtaat caacgcatcg
     2701 ccgcgactat tcgtcataat cgcgctcgtg gacgccatca aatcaccgcc atgtcggaaa
     2761 tcgtccgtga acttagccag ttgggatggg acgataataa aattggcaaa gagctgggaa
     2821 tggacagcga cgaagtgctg cgcctgaagc aaattaacgg cctgcaagag ttgtttgccg
     2881 accgtcaata ttcccgcgcc tggacggtta aatagctaaa gctggcgcag gcgttcagct
     2941 gctgccagca acgtcgattc cttcttggca aaacagagac gaatcagttt atgagggaag
     3001 ggatcggcgc aaaacaccga tagcggaatc gccgctacgc cgtgctcctg cgtcagccac
     3061 tggcaaaact caacatcatc cagggtagat acggcgctgt aatccaccag caaaaagtat
     3121 gtaccttcac acggtaaaat ctccagccgg ctttcattta aagcattcac cagaatatcg
     3181 cgcttctggc gataaaagtc cggtaacgca agataatgct caggttctgc acgtagcata
     3241 tcagcaagcg ccagctgtgc cggggtattc accgaaaagg tcagatactg atgtaccttg
     3301 cgaatttcgg cactgatggg cgctggcgca acacaataac ccactttcca gccggtcata
     3361 tgataggtct tgccaaatga agaaaccgcc actgcccgct cacgcagctg cggatgcgcc
     3421 agcacactgg catggccctg ttgtgaaaag ttgatgtgct cgtagacttc atcgctaatg
     3481 acaaaaatct cgtgcccggc gatcgcctgc cacaaagcgg cgaaatcagc ctgctgccag
     3541 acagttgcac tgggattatg cggagtgttg aggatcacca gtctggtgcg ctcgcttaac
     3601 aatgcggcaa attcctgcca gtcaacgcga aaatgcggtg gttgcagtgc catacgcttc
     3661 actattcccc cagaaagcgc gatggcgggg gcgtaactgt catagctggg atcaaaacaa
     3721 atcacttcat cgccattgcg caccagtgcg gtaatcgccg cgtataacgc ttccgtcgcc
     3781 cctgccgtta cggtgatatc gctatcggca tctggttgat agccatacaa acgttccgtt
     3841 ttctgagcaa tcgcctcgcg caaggcctgc acgccggtca tgggcgcgta ttggtttgcc
     3901 ccctgggcaa cgtggtacgc cagccgctcc tgtaaatagc gcggaccatc aaaatcagga
     3961 aagccttgcg acaggttaat cgcctggtgt tgctgcgcca gcgcgctcat ctgggtgaaa
     4021 atagtggtgc caagttgtgg aagtttgctt tgtggaatca gagggttatt tgtcattttt
     4081 attgtacctg acgaagttgc gtgttgaagg cactataaca cgatgctagt atttggcaat
     4141 caagacgttt agatgtctaa ataaaacaat aaggacaaca caacatgcct cacaatccta
     4201 tccgcgtggt cgtcggcccg gctaactact tttcacatcc aggaagtttc aatcacctgc
     4261 acgatttttt cactgatgaa caactttctc gcgcggtgtg gatctacggc aaacgcgcca
     4321 ttgctgcggc gcaaaccaaa cttccgccag cgtttggact tccaggggca aagcatattt
     4381 tgtttcgtgg tcattgcagc gaaagcgatg tacagcaact ggcggctgag tccggtgacg
     4441 atcgcagcgt ggtgattggc gtcggcggcg gcgcactgct cgacaccgcg aaagccctcg
     4501 cccgccgtct cggtctgcca tttgttgccg ctccgacgat cgccgccacc tgcgccgcct
     4561 ggacaccgct ctccgtctgg tataacaatg ccggacaggc gctgcattat gagattttcg
     4621 acgacgccaa ttttatggtg ctggtggaac cagagattat cctcaatgcg ccgcaacaat
     4681 atctgctggc ggggatcggt gacacgctgg cgaaatggta tgaagcggtg gtgctggctc
     4741 cgcaaccaga aacgttgccg ctaaccgtgc gactggggat caataatgcg caggccattc
     4801 gcgacgtctt gttaaacagt agcgaacagg cgctgagcga tcagcaaaat caacagttaa
     4861 cgcaatcatt ttgcgatgtg gtggatgcta ttattgctgg tggtgggatg gtcggtggtc
     4921 tgggcgatcg ttttacgcgt gtggcggcgg ctcatgccgt gcataacggt ctgaccgtgc
     4981 tgccgcaaac cgagaagttt cttcacggca ccaaagtcgc ctacggaatt ctggtgcaaa
     5041 gcgccttgct gggtcaggat gatgttctgg cgcaattaac tggagcgtat cagcgttttc
     5101 atctgccgac tacgctggcg gagctggaag tggatatcaa taatcaggcg gagatcgaca
     5161 aagtgattgc ccacaccttg cgcccggtgg agtccattca ttacctgcca gtcacgctga
     5221 caccagatac gttgcgtgca gcgttcgaaa aagtggaatc gtttaaagcc tgacgtgcaa
     5281 atttagcagc agcgcgcgcc gccttttcca ccgtagcgcg cgtcctgccg ctcacggaaa
     5341 aactcctcgt aggtcatcgg cgtttgatcg ggatggttaa cccgcatatg ttcgacatag
     5401 ttgtcgtaat caggcatacc aatcatcagt ttcgccgcct gacctaaata ttttccggct
     5461 tttgccagtg aatcaaacat cttggcctcc gctaaccatg ttgctgaaag agtgccggat
     5521 gcggcgtgaa cgccttatcc ggcctacaaa tgctatcaac gcgctcggtc agtcccctcg
     5581 ccccatcggg gagagggtta gggtgagggg gaacccacgg cgccatgcca acattcgcca
     5641 acatcccccc tcactctgac tttagtgcgc gccttttgcc tgcgccacga tctcctcgac
     5701 attttccggc attggctcat acggcatttc tttcgccgtt ggcttcggct ctttcaatgc
     5761 cgcaagtgcc gtcttaatcg agaacagtgc cagaaccacc acgaccacca taaagaagat
     5821 ggttaacccg gcatccagac ggttgttgaa caccagttgc gccagctgtg actcagtata
     5881 ctgcgacgga atattgccgc tgtcgatcat tgcctggaac ttgttagcaa tggccaggaa
     5941 gccgactttc gcatccgggc taaacgcttt ctgccagcca gcggtcaggg tacaaatcag
     6001 cagccaggcc gttggtacca gcgccaccca ggcgtaacgt tgacgtttca tcttgaacag
     6061 caccacggca cagagcatca gcgccatccc tgccagcatc tggttggcaa taccaaacag
     6121 cggccacaga gtgttaatac cgcccagcgg atcgaccacg ccctgatgga ggaagtagcc
     6181 ccacgctagc acgcacagcg ccgttgccag caggttagca ggcagtgaat cggtccgctt
     6241 caggccagga gacaccacgc ccagcagatc ctgcaacata aagcgcgcag cacgcgtacc
     6301 cgcatctacc gccgtcagaa taaacaatgc ttcaaacaaa atggcgaagt gataccagaa
     6361 cgccacatcc atcatgccgc ccagcgcgcc gtgcagaatg taggccatcc ccaccgccag
     6421 cgtcggcgca ccgcccgcac gggaaatgat cgactgttca cccacttcgc tggcaatctg
     6481 gtttagcgta tccggggtaa tcgcaaagcc ccagctactc accacttgcg ccgcagaagc
     6541 gaccacatcc gccgtccctg ccggagccag caccgccatc gggctgttca tggcgaaata
     6601 gacgcccgga tcgatgatac aggcggaaac cagtgccata atcgccacga aggattccat
     6661 caacatcccg ccgtagccga taaagcacgc ctgcccttcg ttcgccagca tcttcggcgt
     6721 ggtaccggaa gagatcagcg catggaagcc agacaccgca ccacaggcga tggtgataaa
     6781 caggaacggg aacaggttac cggtccatac cgggccagtg ccatcgacaa tttggtcagc
     6841 gcaggcatgg tcagcgtcgg acgcataatc aaaatgccta ccgccagacc gacgatagtc
     6901 ccgattttca ggaaggtaga gaggtagtca cgcggggcca gcagtaacca caccggcagc
     6961 accgccgcca caaaaccgta gcccaccagc atccaggtca gctgcacgcc ggtaaagtca
     7021 aagtacggtg cccaggtcgg gctttctgcc acccagccgc cagagataat ggcaaaaatc
     7081 aggaacacca ggccaatcac tgacacttcg ccaatacgcc ccggacgcag atagcgcagg
     7141 tagattccca taaaaatcgc cagcggaatg gtgaacgcaa cggtatacgt tccccacggg
     7201 ctatgagtca gggctttcac cacgatcatc gccagtactg caaggataat gaccatgatc
     7261 ataaagcagg ccaccagcgc gatcaccccg gcggttggcc ccatctcttc tttgaccagc
     7321 tcacccagcg aacgaccgtc acggcgcgta gaaacaaaca gcaccatgaa atcctgcacc
     7381 gcaccggcga gaaccacccc ggcgagtagc cagatcatcc ctggcaggta acccatttgc
     7441 gccgccagta ccggcccaac caacggacct gctccggcaa tggccgcaaa atggtgaccg
     7501 aacagcactt tcttgtccgt cggcacatag tccagcccgt cgttatggcg caccgctggc
     7561 gtcatacgcg tcgggtcaac cgccagcaca tttttggcga tatacaggcc ataaaaacgg
     7621 taagcgatca gatagataca gaccgacgcc accacaatcc acagcgcgtt gatctgttcc
     7681 ccacgattta aagcaatgta tcccagagca aatgctccca ttacagagag cactgtccag
     7741 acgaggtatt tccctgattt gttcatagtt gttatccgtg tgcgtgtcca tagagatgtt
     7801 acattttgtt tctataacat ctttgtacga tttaacaaca cggcaaccat gtaaatcgga
     7861 gttgaaacca atatttaact taacaatgtt aactcgatca ctccgttaac cgagttttcc
     7921 tgcaatctca tttaattctg tcgggctcat cccaaaactg ccgtacccag ccgacaagtg
     7981 caacaacgcc gcccctgttc atcgaaaacg acgatttccc agctctgatt ttgccgccca
     8041 agatgcagcg gctggcagac gccgcgtacc ttgccttcag acaccgggcg atgatgggtg
     8101 gcgttaagtt ccgtgcccac cacacactgc ccgtcacggg tcatcataaa tccggccatc
     8161 gatcccagcg tttcagccag cgcagccgac gcgccgccat gcagcaggcc aaacggctga
     8221 tgagtacggg tatcaaccgg catttcggct tccagcacat catcgcccag acgggtatac
     8281 acaattccca gatgcgccac cattgtgtta tcgctggtgg cgttcagttc gtcgagcgtt
     8341 aaatggcgtt tccagatcat gcttatgccc ccagcgttga gccgccatcg accacaatat
     8401 cctgtagggt gatatggctg gcgaggtcag aggcgaggaa caaaatcgtg ttggcgatct
     8461 cttgtggacg ggcgattttc cccagcggaa tgccgagttt aaactgctcg ccaaagccgc
     8521 gaatacgctg ttcttcggcg tcatcgctca cccacagcgt gcgttgcata tcggtatcgg
     8581 tggaacccgg cgaaaccaca ttacagcgca cgccgctacc cgccagttcc agcccgacgc
     8641 tcaacgccag gcttttcagc gccgctttcg atgcgccata agcactcata ccaatacgcg
     8701 gcgtgtgtgc ggcgtcggac gccacagtga caatcgcccc gccccgctga cggcgaaact
     8761 ggttcatggt ttgctggaac aggttaaacg caccgccgac gttaaccgca aaagtctgct
     8821 gccagtcctc cttactgagc tgatcggtcg cgcccatgcg caaaattccc gccgcattga
     8881 ccagcacgtc cagtcgctcc gtttcagcta acagtcgctg acatacctgc gccacctgcg
     8941 cagcatcggc aacatccatt acttcggtcg caaagggata ttgctcctga gcgaacgctt
     9001 gatcaaaacc tgtaactttc gctcccgctt caacaaacgc cagcgccgtg gcgtagccga
     9061 tacctttccc cgcgccggtt acccagacat ttttaccgct gaaatccatt atttcacctc
     9121 gcgggagagt agcttccacc aggcgtcgat ggtcgggttt ttcgccaaca tgacaaagtc
     9181 gatatcgcca tgcactttgc gccagcgcgc cgccagcgcc atcatgcgca ccgaatccag
     9241 accgtagtcg atcaggttgt cgtcatcgaa cggttcatcg gactcgtcca gcaacggcag
     9301 gatcacctca cgcagcgccg ctttgctggc ggggataggt gctggcagta attcttcggt
     9361 catcaccacc cggccagaac gtccggccac atatttcagc gacatcaaat gctcgtcacg
     9421 gctgaaatcg gccagcgcat ccgccaccat aaacggttta atatcgcgca taaatgcgtc
     9481 ggttgcggtg gtcatacagc caatgtgggc atatacccct gtaataatca gctggttacg
     9541 tccactctct ttgagcattt gctccagcgg agaacgatga aacgcgctgt agcgccactt
     9601 caccagcacc gtgtcgtcgg catctggcgt caggcgatcc accacctttt gctgttccgg
     9661 cgagcgggtc aggcccggcc cccacatatc attcaacagc gcccgatctt catcgctctg
     9721 ctctttcggc tgggcggtgt aataaaccgg gatattgtgc tgtttgcagt agtcgcgcag
     9781 cgcagcaata ttcgcgatca cctgctccat catcgggcag ttctcgcccc agaagctgac
     9841 aaaatagtcc tgcatatcat ggattaacaa cgcggcacgt tgcggttcaa aggcccagtc
     9901 gactttattc ttagggatat cgtgagcctc cggcagtgcg taagcctgta attttggaat
     9961 agccatcgtg ttctctcctt caggctgatg cgcgtgacgc cagccactga cgtaattgtt
    10021 ttttatcgac tttcccgacc gccgtcagcg gaagtgaatc cacacactcc acgcgatccg
    10081 gtaatttaaa ttcggcaata ccctgttcac gcaggaaacg acgcacctgc accgcgcgca
    10141 gcggctcttt taccaccaga taagcacagc ttttttcgcc catcagctca tcttccatac
    10201 tgaccagtgc ggcgtagatc accgccggat ggcgcagcaa caggttttcg atctcttcgg
    10261 cagcgatctt ctcgccgcca cggttgatct gatctttctc gcgcccctgc acggtgatgt
    10321 aaccctctgg atcaatagag atcagatcgc cggaacagta aaaaccgttg gcatcaaagg
    10381 cgctggcatt gtgctgcggg cttttgtaat agccacggaa ggtatacggc ccgcgcgtca
    10441 tcaggcgtcc gacttcccct tgcggcagtg gatttccttc ggcatcggca acccatactt
    10501 cgtcatccgg acacattggg taaccctggg tatggataat tttctccgcg ctatcatcaa
    10561 gacgggtata gttcaccagc ccttccgcca taccaaacac ctgctgcaac tggcaaccaa
    10621 tctcagcggg aatacgcgcc gcaagcgtgg cagaaagacg tgcgccaccg acctgtaaca
    10681 gtttcagcga ggcaagctgc gcccggcttt cgccttcggt cagcgcctgc aaccacaggc
    10741 tgactgccgg cggcaccagc gcggtaacgt taatctgatg tttttcaatc aatgggaagc
    10801 aaagcgtggc gctgggatcg gccgccagaa caaccgttcc tccggcaaga aagacgccca
    10861 gcgatcctgg cgaactcatg gcgtagttat gagccgccgg gatcgcgcac aggtagcgcg
    10921 tctgttgtgt gaactgacaa atctcgacgc tacgacgcac gctgtagtag tagtcgttat
    10981 gagtgcgcgg gatcagtttc ggtgtgccgg tggtgccgcc ggaaagctgg aaataggcca
    11041 cttcatcagc aggtgacggc gtggcagtaa aatcctcagc cggatggtta atcgcatcct
    11101 gcaagttatg ctcaccgctg tcgttgtgca gttgcaccac gcgaatggag gagtgctctg
    11161 caataaatgt attgaggaaa tcatccccgc taaatagcgc atgttggcga tcggcaatca
    11221 gtaacgccgg ttcaatctgg ctggcatagg cgttcagttc actacgctga tggctgaaca
    11281 gcgccagcac cggcgcaacg cccagtttca gcagcgcgaa aaaggtaatg taaagttcag
    11341 cgacgttacc cagttgtacc agcgcggttt caccgggttt aatgccctga cggcgcaaac
    11401 tacaggcgag attattcgcc gcctgattca gctcccgata actcaactgc cgctcgccgt
    11461 cgataaccgc gatgctgtca ctcgcagcgt ggcgagtcag aatgtcagtc agtggcaaat
    11521 cctgccagta gcctttttcc cgatagcgac gggcaaactc ttccggccag cgggtaaatg
    11581 gaatgctcat catcgctcct taatgcaatc caaaaacgtt caacatggta gaaagtttga
    11641 cgcctgtttc gcgccactca cccaacggtg acgacgcagg cacaatcccc gctccggcaa
    11701 acagacgcac ctgattttcc cgcagcttcg cgcagcggat ggtcaccacc cattcgccgt
    11761 taccttcgct gtcacaccaa cccacaatgc cgccaaacag ttcgcggtcg aacggctcca
    11821 gttcagcaat aacctgggtc gcggcctgat gtgggaaacc gctcagcgcg ggggtcggat
    11881 gcagcagaca agccagagtc agtgcgtttt cttgcgaatt cgctttacct tcaaagggag
    11941 ttgcgagatg ccacagcgtc ggcgtggtaa tcaattgtgg agaggaggga acgtgtaact
    12001 cactactgcg ttcgcgcagt acctctttca tcgcctgagt caccagttca tgttcatggc
    12061 gatctttttc tgacgccagc agacgattac ccgcttcgcg atcgagcact tcatccggct
    12121 gacgacgcgc ggaaccggct aacggaatgg agctaaaacg ctcgccgtct ttacgtagca
    12181 gcagttccgg gctggccccc agcagaacgc caccatctgc caacggcaca tggaagttgt
    12241 aactaaccgg gttttgcgca atcaaccgtt ccagcaatac gccactatca atggcggcgt
    12301 cagtggtgat atcaatcaac cgtgacaaca ccactttgtc gacctgcggc gtggcggtaa
    12361 gtgcggcggc gcgggcaacc atctgttcaa acgtggtttg ttccggaatt gcctggcgtt
    12421 ccaccacatt cagcaactgg ctgcgggtga aacggcgtgc ggaagcttgt ttttcctgac
    12481 gggagaacga ctgccaggat tcgggaatat acagcgacga aggctgacgt ggatcgaagg
    12541 gaatcgcccc gaccatcacc ggatttttga tgccctgcgc tttggcatcg gcaaacagcg
    12601 cggcgagttt ttgctggaag ggactgtcag gcgaatcccc gttcacagcc ggttcatcga
    12661 agcgggcgaa acatcctgac gtcgtaaaac tgcggtacgg cgacataaag aaaaagcgat
    12721 tgggcgcaag tgttgccatg gtctgctgta cttcctcagc cagtgacgta tccatatcat
    12781 cctccacaaa atgataaagg ctttaataat gattatcatt tatattttcg gtcgctaacc
    12841 taaaagcaaa cgcgcactat gtcaactctt gaggtaacgc aatctgcaac tcggggttgc
    12901 atctggttgt ggttacaatg aaaatgagaa gcattaactt cattaataac aggaagctga
    12961 tttgtgagac tcgccccgct ctaccgcaat gcccttctat taacaggact tttgctttca
    13021 ggaatagccg cagttcaggc cgccgactgg ccgcgtcaga ttactgacag ccgtggcatt
    13081 cataccctgg aaagccagcc gcagcgtatt gtttccacca gcgtcaccct gaccggctca
    13141 ctgctggcga ttgatgctcc ggtgatcgcc agcggcgcga ccacgccaaa taaccgcgtc
    13201 gcggatgacc agggcttttt acgccagtgg agcaaggtgg cgaaagaacg caaactgcaa
    13261 cggctctata tcggcgaacc gagtgcagaa gccgttgccg cgcaaatgcc ggatctgatt
    13321 ttaattagcg caaccggcgg tgattcggcg ctggcgctgt acgatcagct ttccaccatc
    13381 gcgccgacat taatcatcaa ttacgacgac aaaagctggc agtcgctgtt aacgcagctt
    13441 ggcgaaatta ccgggcatga gaaacaagcg gcagagcgga ttgcacagtt tgataagcaa
    13501 ctggcggcgg cgaaagagca aatcaaatta ccgccgcagc cggtcactgc cattgtctat
    13561 accgccgctg cacacagtgc caatctctgg acgccggaat cagcacaagg gcagatgctg
    13621 gaacaactcg gctttacgct ggcgaagttg cccgcaggct taaacgccag ccaaagccag
    13681 ggcaaacgcc atgacatcat tcagcttggt ggggaaaatc tggctgcagg gttaaatggc
    13741 gagtcactat tcctgttcgc cggtgatcag aaagatgccg atgctattta tgctaatccg
    13801 ctgctcgcgc acctgcctgc agtacaaaac aagcaggttt atgcgctggg aaccgagacg
    13861 ttccgtctgg attactacag cgccatgcaa gtgctggaac gacttaaggc gctgttttaa
    13921 gcattaaccg tcagacgctg tcacctgcgg cggcgtctgg cgaaaacgtc gcaactccac
    13981 cagcaccagc aataacaaca cgccgataat caacaaacca aaaccgcttg cgcttgcgga
    14041 agcaaccggt gtcatcatcg ctcccaaacc gcccagcagc gccgcgccta tggcatcgcc
    14101 cgtcacgttt tgtgccgtcc acaaaccgtt aatccgccct aacatcgctt ccggggtttg
    14161 cgtttgcagc attgtgtatt gcagcaacga gctgaccgca ctcaaccagc cgaacagcgc
    14221 cagacaaacc acgcctaaaa tccacatcgg catcaggcca aacagaccaa tggcgaggaa
    14281 cgatcccagc gtggagagca gcatcaataa ccctggtcgc gcgctatgcg ccagcttccc
    14341 gctggttaac gcgccaatag ccgcgccgag cgggatcgcc gcatagagaa aaccaatctg
    14401 tgccgccgac atctgccagt tgtcagccag cgccggatac agtacccgca ccgcactcgc
    14461 catcgtcaat aaaccaccca gcagcgcaat ccctcctacc agcgggctgg cgagcagaaa
    14521 acgaaatcct gccagtaatg atttcaacgg atgctcacgc ggctgcggtg gcggtggcaa
    14581 cgccggaagg cttaacaacg gcagcaaggt aataaacgtg cccgccgccg ccagtccgta
    14641 gttccaggcc acaccgccag tcgccagcag taaaccgcca atcatgggcg aaatcaccga
    14701 ccccagacgc acggtcaaca tggtgatcgc cccggcctgc attaagtttt cacgccctac
    14761 cagtgcaggt gtcgccgcca gtagcgccgt aacgccaagt gatgcgaaaa aaccatccca
    14821 taaaccaagt aaatagattg ccagcaatga cggttcaggc agcagtgcat taaggcacag
    14881 tccaatgaag ccaatgccac aggtgccgcg cgccagcaaa atcacttttt tgcgttcata
    14941 gcgatccgcc agcacaccgc cgaccatcag gccaacaaac atcgcgccgc cggtcagcgt
    15001 caccgaaagc cccacctgcc aggtggaatg tgtcatcatc tggatctgca ccggcaccgc
    15061 gacgccgagc aaacccagag aaacaataga gatgaaacga gcgaggaata ctgcgcgaaa
    15121 cgccgggtgc gttttcaaca ggctgaggtt aagcagccag gattgtttat tcattacaat
    15181 gccttgccat caaatatttt aaatacctat tcttaggctg cacatgctaa catatccaaa
    15241 taagatcgat aacgataatt aatttcatta tcatggaagt tcgtatgtct ggttctgttg
    15301 ccgtgacacg cgccattgcc gtgcccggat tgctgttatt actgattatc gcgacggcat
    15361 taagtctgct cattggggca aaatcactcc ccgcttccgt agtgctggag gccttctccg
    15421 gcacctgcca gagcgccgac tgcaccatcg tgctcgacgc gcggctgccg cgtacccttg
    15481 ccggtttact ggcaggcggc gcgcttggcc ttgccggggc gttaatgcaa accctcaccc
    15541 gaaacccact tgccgacccc ggcttgcttg gcgtgaacgc cggagccagc tttgccattg
    15601 tgctgggtgc ggcgctgttt ggttactctt ccgcacagga acaactggcg atggccttcg
    15661 ccggggcgct ggtggcctcg ttgattgttg cctttaccgg cagccagggc ggcgggcagt
    15721 taagtccggt gcgtttaacc ctggcgggcg tggcgctggc ggcagtgctg gaagggctga
    15781 ccagcggcat cgccctgctt aatcctgacg tctacgatca gttgcgtttc tggcaagccg
    15841 gttcgctgga tattcgcaat ctacatacct taaaagtggt gctgatcccg gtgctgatcg
    15901 ccggagcaac cgcgctatta ctgagccgcg cgctgaacag tttgagcctc ggcagcgaca
    15961 ccgcgacggc gctgggcagt cgcgtggcgc gcacacagtt gattggtctg ctggcgatta
    16021 ccgtgctttg tggtagtgcg acggcggtag ttggcccgat tgcctttatc ggcctgatga
    16081 tgccgcacat ggcgcgctgg ctggtaggtg ccgatcatcg ctggtcgctg cccgtcacgc
    16141 tacttgctac ccctgccctg ctgctgtttg ccgatatcat cgggcgggtg attgtgcccg
    16201 gcgaactgcg cgtttctgtg gtcagtgcgt ttattggtgc accggtgctg atcttcctcg
    16261 tgcgacgtaa aacgcgaggt ggtgcatgat ttacgtctct cgccgattac tcatcacctg
    16321 tttgctgctg gtttccgcct gtgtggttgc aggtatctgg ggattacgca gcggtgccgt
    16381 cacgctggaa acctcgcagg tattcgccgc gctgatgggc gatgcgccgc gcagtatgac
    16441 gatggtggtc accgaatggc gtttaccacg cgtgctgatg gcgctgttga ttggcgcagc
    16501 actgggcgtc agtggcgcga tttttcagtc gctgatgcgt aacccgctcg gcagccctga
    16561 cgtaatgggt tttaacaccg gggcgtggag cggcgtgctg gtggcgatgg tgctgtttgg
    16621 tcaggacctg acggctatcg cgctggcagc aatggtgggc ggcattatca cttcgctgtt
    16681 ggtctggctg ctcgcctggc gtaacggtat cgacaccttt cggttgatta ttatcggtat
    16741 cggcgttcgc gccatgctgg tggcctttaa tacctggctg ttgctgaaag cgtctttaga
    16801 aacggcgcta acagcaggtt tgtggaatgc cggatcgctc aacggcctga cgtgggcaaa
    16861 aacctcgcct tccgcaccca tcattatatt gatgctaatt gccgccgcct tactggtacg
    16921 ccggatgcgc ttgctggaaa tgggcgatga taccgcgtgt gcgctgggcg tcagcgtcga
    16981 acgttcgcgt ctgttaatga tgctggttgc agtggtgctt accgctgcgg caacagcgct
    17041 tgccgggccg atttccttta ttgctttagt cgcgccgcac attgcccgac gcattagcgg
    17101 caccgctcgc tgggggctaa cccaggcggc gctgtgcggg gcgttgttac tgctggcggc
    17161 cgatctctgc gcccaacaac tgtttatgcc gtatcaactt ccggttggcg tcgttaccgt
    17221 cagcctcggc ggtatttacc ttatcgtctt gttaattcag gagtctcgca aaaaatgacc
    17281 gaatcagtag cccgtttgcg cggcgaacag ttaaccctgg gatatggcaa atataccgtt
    17341 gcggaaaatc tgactgtaga aatacctgat ggtcacttca cggcaattat cgggccaaat
    17401 ggctgcggta aatccacgtt gctgcgtacc ttaagccgcc tgatgacgcc tgctcacggg
    17461 catgtctggc tggatggcga gcacattcaa cattacgcca gtaaagaagt tgcacgccgg
    17521 attggtctgt tggcgcaaaa tgctaccacg ccgggcgaca tcaccgtgca ggagctggtg
    17581 gcgcgtgggc gttatccgca tcaaccgctg tttacccgtt ggcgcaaaga agatgaagag
    17641 gcggtaacga aagcgatgca ggccacggga ataactcatc ttgcagatca aagcgtggat
    17701 accctttccg gtgggcaacg ccagcgagcg tggatcgcga tggtgctggc ccaggaaacg
    17761 gcgattatgc tgctcgacga acccacgacc tggctggata tcagtcatca gattgatcta
    17821 ctggaactgt taagcgaact gaaccgcgag aaaggctata ccctggcggc ggtgctgcac
    17881 gatcttaatc aggcctgtcg ttacgccagc catttgattg cattgcggga agggaaaatt
    17941 gttgctcagg gagcgccgaa ggagattgtc actgctgaat tgatcgagcg catttatggt
    18001 ctccgctgca tgattattga cgatccggta gccggaacgc cgcttgtggt gccgctcgga
    18061 cgaacggcac cttcaaccgc aaagatttaa actaagtggt ctgccatcat ggcatcctgc
    18121 tttctggatg ccatcgcata gcgcaataac acgctaccac aagccaccat cccgccgatc
    18181 agcgcggaaa ggatcacaat aatcgcctta cccggaccgt cttttttcac tggcaatgac
    18241 gggcttaact gatatttaaa cggcgtaaaa ttcacatcgt tgatatttgc ttttgttaac
    18301 tgctcaacaa gatactgacg attacgtaat tcaccgttca gttccgcaac gtcagtgacc
    18361 gctttttcaa tttccagttt gcgttcaata ccgtctgcgc cgagagaaat agagaaatcg
    18421 gggtcatctt taacggcctg accattactg taaacgggct ttttaattcc tgccgcgttg
    18481 gcaatgtcga gtgaataatt gaggcgctga atgtttgcat caagttgatt tttcatttta
    18541 atgcggtcct gagccagttt ttctttttca aactgggttt tgatctccag tttattacgg
    18601 acgttttcta tcgactcttt caccaccaac gcagagatat aatcgatata cccgctcaaa
    18661 acggtctgcg cctcttcact ggttggcgcg gtaaaactta gcgtccagga ggtatacagt
    18721 gacggttcat cttttttctt actggcattg tcatcaaccg ctttcatttt ttcgctcaat
    18781 gcgacaattg cgcgatgcaa atccagttcg tcgattttcg cctcttttaa ttggtccatc
    18841 acataaggtg atgaacgcag gtactcttcc agcaagctaa ccgactgaaa cttcttgata
    18901 aacaggttaa atgcttctgt acgatcaatt ttgatatcca gatccagcac acgaagctta
    18961 gtgaatgttt tctccaactc ttgccactga acaggttctg gaggcgtgac aaccgccgcg
    19021 ctggtccatt tttgcggcag gatgaaagag atcagcaagc ctgcgcaggc aaacgcaaaa
    19081 acgaccgcca tgaccgtttt tttggcccgc cataaaactg agattagatt aagtaaatca
    19141 atttcattat tactgggcga cgccagagga taatcgggaa aatgagcgtc acttccctgt
    19201 ttaatattca gtgatgacat ggggtataac ctgaaaatga gtccaatagg gggaaatctc
    19261 cacggcgctc actttaccag ttggtgagaa ttatccgaag ctgaagtttg taaattcatt
    19321 ccactgaatg caacattcac tctatttaag aaaaataacc agcaaactta taaattatat
    19381 aaatgataaa cccataatta tttcctgcaa ttatgggttt atagataatg ttaatttatc
    19441 tgtttagcgt tgcgcgaata atcggcccaa ttttttcaaa tgcccctgga gagataatat
    19501 ccacatgcgc acaatcctga cgatagatat ccagctccgc aatccacggc gaccaggcgc
    19561 gttcaggact cataccttcc tgaagcgtgc gttcagcaac aaacaacgtc gctttaccgt
    19621 caaacggtac gctatgagca gtcgtcagca ggcgcacagc atcagcgtag ttgccttcaa
    19681 tggtggtaaa caactccgtt gaagtacttc cctgctgtgc cgccaggaag gcctcgcgct
    19741 cgcggttaat ctccgccagc acttccgggt ccagaccatt agcttctttt tcctgccagt
    19801 tttgcgtttc tggcggccag gtatccagca agccaagaaa tgccacctgt tcgccacggg
    19861 cacgcagccg cgccgcaata ccttgcgcca gcgtaccgcc aagtgaataa cccagcaggt
    19921 aataagggcc gtgcggttgt tgttcaagta acgttgccag atgcgcttcg cagacttcat
    19981 ccaggttcgt cgctgtctgc atggggccat gagggcgcgg cgactgaatg ccgataatcg
    20041 accatagtgg atcgagataa cgcgagagca cgctaaactg ccaggcaaaa ccggatgccg
    20101 gatggaaaca aaacagcgtc gggccattac cttcacgcaa cggcagaatg gtttcgaatc
    20161 ccatgcgccg ggagctgtcc tcttcaccat caataatcgt tgccagtttg gcgacggttg
    20221 acgcgaccat cacctgcccc ggcgtcacct gacgggcaaa ctgccgactt aactgcgctg
    20281 ccagtttcat tgccagtagc gaatgaccac caagcgcgaa gaaatcagca tcgacatcct
    20341 gcacgtcaca acccagtaac gacgagaatg ccgcggcgat aatcgtttca ctgcccgctt
    20401 tcggcgcacg ccccggcgtt tgtgccttaa gttcaggcaa cggtaaggct ttgcgatcca
    20461 gcttgccgtt ggcgctaaga ggtaactgtg gaagttgcag cagaacgacc ggcaccatat
    20521 gcggcggcaa tgtttcgcga agctgtgcct gtaatgcgct ggtatccaac ggcagacctg
    20581 attgcgacac cagatagccc accaactgac gcgcatcacc accggtggct gccgcctggt
    20641 taatcacaca ggcgtgggta acggcttgtt cgacatccgg cagcgcctgc atcacgcgat
    20701 cgatttcgcc cagttcgata cgctgcccgc gaatttttag ctgatcgtca ctgcgcccga
    20761 ggtactccac cgcgccgtta tccagccagc gggcaacgtc tccggtacgg tacatccgtt
    20821 cgccaggagc aaaaggatcg gcaataaaac ggctggcggt cagatcgggt cgtccaagat
    20881 acccctgcgc cagttgaatg ccggtgagat agagatcacc cgccacaccc ggcggcaccg
    20941 gatgcatcat cgcatcaaga atacgcagac ccgtattcca taccggataa ccaatcggca
    21001 cactgctgcc gcgcacctgt gccagttcct cgccaaaagc cggataccag ctgacatcta
    21061 ccgccgcttc cgtcgggcca tatagattat gcaacggcgc gcccgttaac tgttgccatt
    21121 cgcggcataa atcagccggt aaggcctcac cactacagaa aacctgtttc aacgtcgcgc
    21181 aactctggcg agcggtttgc ggagtcagcg atgcaacaaa tgccgccagc atcgacggca
    21241 caaagtgcgt ggtcgttacg ccatattcgg caaagaattg ctgcatagcg agcgggtcgc
    21301 ggtgcgcttc cggttcagcc atcaccagtt ttgccccggc aataaacggc cagaaaaact
    21361 cccacaccga gacatcaaaa ctgcacggcg ttttttgggc aacgacatct tcgcctgtaa
    21421 gcgggtagtg attttgcatc cataacaggc ggttaacgat agccgtctgc ccgaccatta
    21481 cccctttcgg tctgccggtg gaaccagagg taaagatgat ataagccgtg tggtgcggtt
    21541 gtgaaagttg cagcggcgca ctgccctgcg gtgtaagcgg ggcgttatag caaaggttgg
    21601 ttaaatctgg aacatcggca aagcgcggca gttgatcatc ggtggtgatt aacagcgacg
    21661 gacgcgcatc ttccagcatc attttcaggc gatcgtccgg ataccccgta tccagcggta
    21721 accaggccgc acctgcttca acaatcgcat gtagcgccag ggtcaaaaag accgagcgcg
    21781 gtaatgccac cgccacactg tcccctggtt taacgccgtg ctcacgcagc agattcgcca
    21841 gcgccaccac ctgctcgcgc atttcccgat agctgaactg gtaacgcgca tctgccagcg
    21901 ccggagcatc cggtgttttt gccgcttgtt ctgccaccag cgcgctaagc gtggtttctg
    21961 gaatctcaac ctgagtggcg ttgatctgcg ccaactgcgc atactcacct ggcagcataa
    22021 tatcgacatc gccgcacaac agcgccggat ccgcggcgaa ctgggcaatc agcattttca
    22081 ggcgttcaga atgctggatt aacgttggct catcgtaacg ctgtttattg gcaaggatct
    22141 caatactcaa atcaccgtgt tcatccggga acagggccag ttcaaggtca ttaaccggcc
    22201 cggttgccag ggtatgggtt tgcgcctgaa caccaggaat gtccagttgg taatcaaata
    22261 ctttgatatt gagtaccgga ccgaacagcg gttcatcacc tgccgctcgc ccactgtcac
    22321 ggacaatttg ttcggcatcg taacgttgat ggcgacgcat ttttttcagt tgtgctgcca
    22381 gtcgggttgc cagttccggc agcgtttctt gcgccgcaat gtgaataccc aacggtaaaa
    22441 cgttgagcac gggtccggta gccgtcagcg ccgccgagcc cagtcgacgc ataaagataa
    22501 atccggcggc gtagtccatg cgattgcaca atcgccccag ccacaaggct gccagcgcaa
    22561 gggctaaatc ggtacgctgc acacctgaaa gttgcgcagc caactggcgg aattccccgt
    22621 cggtaaattc cagtttcagg cgcagaatat ctgccgaggc gctacgcccc ggtaaaggtg
    22681 ccggagaaag tgacgcgggc ggtggcagtt ggcgacgctg ttccacccag aatgccgcat
    22741 cacgctgcca ggcttcgctt tcgcggtatt gctggtactc ttccactaca tcagcgaaag
    22801 gcgtaaacgg cgaagcaggc gttggttcgc cacgcagcaa tgcgcagtaa atgttggcga
    22861 tctggcgggt aatggccgga aaactgaagc catcgaccag taaatggtga taacgctgat
    22921 accagtacca gcggttatct gccacctgta tcagctggtg aaagaccagt ggtttaccgc
    22981 tatcgacgcg caaatcttgt tgcaaatccg cctgcattaa tgcctgcgca gtaccgtgcg
    23041 gatcaatgtt ggttcgcagg tcgataattt ctggcagttc gaatatcagc gcatcatcga
    23101 cccattgcca gacttcgccg ttatcttccg taaaacgcat ccgcaacgta tctgcttgcg
    23161 ctaatccggc aaccaccgcg cgggccagta atggcgcatc aacctctccg gttaactcaa
    23221 cgtaatgcgc cacgctccag gcggagggta aatccgacag tttttctgcc atccagatgc
    23281 cgggctgtgc ggcgaccaaa ggtaaatgct ggctcattgt gcctcctgca actgggtgaa
    23341 attcgccggt gtcagagtac gccagtgggc ttcaagccac tgctggcagg actcttgtga
    23401 ctgcggctga cacacaatgt cccagcctgc cggtaaggcg cattgttgcg gccacagact
    23461 gaattgcccc tgcgcattgc gcaaaatgta aaacactccc tgtggatcat cgaagggatt
    23521 actgaatgcc atattcaact cctgtcatgg aaaagtggtt gccagaggtc gattaacccc
    23581 tgcatcaagc caccgcgcca acaaagcgca tcatgtccgc cgtcaacctg acgccagaaa
    23641 atggattctt ttatagggtg taattgcgca tacagcgcct gattggcccg catgatcatc
    23701 ggctcgcgaa tacccgcttc cagcacaatg cgcagacctt cggcgctaac ttcaccagct
    23761 tctagctttt caagtaacac gccctcttgc tgcccgcccc gatgcggcca ccagtacgat
    23821 cctgactggc ttaatacaca gccaaagcgt tcaggccagt gcagtccggc atacagcgcg
    23881 gacagcccac caaaactctg cccggcgacc acggtgcgat cggcgcgatc gctaaaaggg
    23941 gcaatagctt tcaccagggg caataactct tgctgtactg ccagccagaa atccgcatta
    24001 cacggcagtt cgtgggcgcg gtgcgtggtg tcgatagcgt cgatcaacac atacacggcg
    24061 ggaggaagtt gctgacgatg ggtcagcgaa gtcagcacag gccagacggg catactttgc
    24121 gcccaaaatt cgccatcgag caaaactgcc agcgggcgtt cttctgctgt tacatcgccg
    24181 gtggtaaaaa tccatacacg acgtgaattt ttcaaccgtt cacttttcca gataatttct
    24241 ttggctggta tttctggcgc ttgcggacaa tcccatcccg gttgcagagg cgcttgcggc
    24301 atttcgagtg cagaaacagc gtgccctcgc ccgcctttcc agctttgtgg attcagcgga
    24361 tcggctatcg cctggggtaa tagttttcgc cagccttcac gtaattcgag gcgatcgggg
    24421 gatggtgcag aaaaaatgtc atcgcgttcg gtgggaataa agcagtagct gccgcgccag
    24481 ttggcattga gttgtgtcgt ccactgccag acatcagtac ctgcaattcg ctgcatcgac
    24541 tggggctggc tgttctgatg atgatcggtc acaccagtga tgtagaccca tacacgcttt
    24601 atcgtcgagt attcttcaga accttgggga tcacgccacc agaaagtgac ctcaaacatt
    24661 tcgtcattca gacgctgcca ttccgggcca tgtttcgact gccaccagct ctcacttcct
    24721 acttttaacg ccgtcacacc ataaccccat gtttactgtg caatttttca ttgattgcag
    24781 aaatatattg ataatattat tgataactat ttgcatttgc aatagcgtaa tggcgcgccg
    24841 tgggaagcgc ggacattaat taaccaactg cactgcgtgt ctttcaggat caaaggtttt
    24901 cgcggtagcg ggatgcgtcg tgttgatgac gaccatgccc gacagttgca attcgtggca
    24961 aaaatgcagg aataaaacaa tgaacaagaa gattcattcc ctggccttgt tggtcaatct
    25021 ggggatttat ggggtagcgc aggcacaaga gccgaccgat actcctgttt cacatgacga
    25081 taccattgtc gttaccgccg ccgagcagaa cttacaggcg cctggcgttt cgaccatcac
    25141 cgcagatgaa atccgcaaaa accccgttgc ccgcgatgtg tcggagatca tccgtaccat
    25201 gccaggcgtt aacctgaccg gtaactccac cagtggtcag cgagggaata accgacagat
    25261 tgatattcgc ggtatgggtc cggaaaacac gctgattttg attgacggca agccggtaag
    25321 cagccgtaac tcggtgcgtc agggctggcg tggcgagcgc gatacccgtg gtgatacctc
    25381 ctgggtgcca cctgaaatga ttgaacgtat tgaagttctg cgtggtccgg cagctgcgcg
    25441 ttatggcaac ggcgcggcgg gcggcgtggt taacatcatt accaaaaaag gcagcggcga
    25501 gtggcacggc tcctgggacg catatttcaa tgcgccagaa cataaagagg aaggtgccac
    25561 caaacgcact aactttagcc tgaccggtcc gctgggcgac gaattcagct tccgcttgta
    25621 tggcaacctc gacaaaactc aggctgacgc gtgggatatc aaccagggcc atcagtccgc
    25681 gcgtgccgga acgtatgcca cgacgttacc agccgggcgc gaaggggtga tcaataaaga
    25741 tattaacggc gtggtgcgct gggacttcgc accattgcaa tcgctggaac tggaagcggg
    25801 ttacagccgc cagggtaacc tgtatgcggg cgacacccag aacaccaact ccgattccta
    25861 tacccgctcg aaatatggcg atgaaaccaa ccgtctgtat cgccagaact actcgctgac
    25921 ctggaacggc ggctgggata acggcgtgac caccagcaac tgggtgcagt acgaacacac
    25981 ccgtaactcg cgtattccgg aaggtctggc gggcggtacc gaagggaaat ttaacgaaaa
    26041 agcgacacag gatttcgtcg atatcgatct tgatgacgtg atgctgcaca gcgaagttaa
    26101 cctgccgatt gatttcctcg ttaaccaaac gctgacgctg ggtacggagt ggaatcagca
    26161 acggatgaag gacttaagtt ccaacaccca ggcactgacc ggaacgaata ccggtggcgc
    26221 tattgatggc gtgagtgcca ccgaccgtag cccgtattca aaagcagaaa ttttctcgct
    26281 gtttgccgaa aacaacatgg agctgactga cagcaccatc gtaacgccgg ggctgcgttt
    26341 cgatcatcac agtattgtcg gcaataactg gagcccggcg ctgaacatat cgcaaggttt
    26401 aggcgatgac ttcacgctca aaatgggcat tgcccgtgct tataaagcgc cgagcctgta
    26461 ccagactaac ccgaactaca ttctctacag taaaggtcag ggctgctatg ccagcgcggg
    26521 cggctgctat ctgcaaggta atgatgacct gaaagcggaa accagtatca acaaggagat
    26581 tggtctggag ttcaaacgtg acggctggct ggcgggcgtg acctggttcc gtaacgatta
    26641 tcgcaataag attgaagcgg gctatgtggc tgtagggcaa aacgcagccg gcaccgatct
    26701 ctatcagtgg gataacgtgc cgaaagcggt ggttgaaggt ctggaaggat cgttaaacgt
    26761 accggttagc gaaacggtga tgtggactaa taacatcact tatatgctga agagtgaaaa
    26821 caaaaccacg ggcgaccgtt tgtcgatcat cccggagtat acgttgaact caacgctgag
    26881 ctggcaggca cgagaagatt tgtcgatgca aacgaccttc acctggtacg gtaagcagca
    26941 gccgaagaag tacaactata aaggtcagcc agcggttgga ccggaaacca aagaaattag
    27001 tccttacagc attgttgggc tgagcgcgac gtgggatgtg acgaagaatg tcagtctgac
    27061 cggcggcgtg gacaatctgt tcgacaaacg tttgtggcgt gcgggtaatg cccagaccac
    27121 gggcgatttg gcaggggcca actatatcgc cggtgccggg gcgtatacct ataacgagcc
    27181 gggacgtacg tggtatatga gcgtaaacac tcacttctga tgctaacgtc agattgttga
    27241 caaagtgcgc gtcgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaaatc
    27301 ttgccaattc aatatattgc aggaccatgt aggcctgata agcgtagcgc accaggcagt
    27361 tttgcgtttg tcatcagtct cgaatatggt cgatatgaaa actacgcata cctccctccc
    27421 ctttgccgaa catacgctgc attttgttga gttcgatccg gcgaattttt gtgagcagga
    27481 tttactctgg ctgccgcact acgcacaact gcaacacgct ggacgtaaac gtaaaacaga
    27541 gcatttagcc ggacggatcg ctgctgttta tgccttgcgg gaatatggct ataaatgtgt
    27601 gcccgcaatc ggcgagctac gccaaccggt gtggcctgcg gaggtatacg gcagtattag
    27661 ccactgcggg gctacggcat tggccgtggt ttcccgtcaa ccgattggcg ttgatatcga
    27721 agaaattttt tctgcgcaaa cagcgaccga attgacagac aacattatta caccagcaga
    27781 acacgagcga ctcgcagact gcggtttagc cttttctctg gcgctgacac tggcattttc
    27841 cgccaaagag agcgcattta aggcaagtga gatccaaact gatgcaggtt ttctggacta
    27901 tcagataatt agctggaata aacagcaggt catcattcat cgtgagaatg agatgtttgc
    27961 tgtgcactgg cagataaaag aaaaaatagt cataactctg tgccaacacg attaattgac
    28021 aacatctggt acgattcgcc cgcagccatc actgaccgcg ggcgaaagtg taaagcaggt
    28081 gccttaccat cctgacctga caaccggata tgcggggatt actccccgca caacggctac
    28141 ttcttcggtt cgtaagcgag aacagccctg aactcaatat tacgttcctt caccgtaaac
    28201 tcacacagcg agtcgccgac cagaagcgta aatcccagca ccgtcaaaca cagcactatg
    28261 actgccacaa gggcatattt cgtcagcatg tttatattgc ctccatgcga agaagggact
    28321 accatccaaa ttgttcaggt ttggagtggc agccccaggg ttgatagaaa tatctcctgg
    28381 ggcttcatct ttccagacct cagagttaac ctgaaaccgg aaagcttcag gcacccgccg
    28441 ttatcttacc cttcctcaac aaaatggctg tttatttata cagtaaatcc tgtttcggac
    28501 ttctcactcc tcatttaatc aactgaagct gagcccgttt tttctgcatg cctcaatcgt
    28561 caaaattctt cgccaccagc cagccgtgcc ataacttgtc acattgcaaa agtagccaaa
    28621 ttgcttcagg caccatcagt tcaccatcac caaaaaaaag aaacattcca gaacttccgc
    28681 atctcgccca gccgctccga cttctatact gaatagaaaa cgccaacata agagaaacct
    28741 atgccattac ccgattttca tgtttctgaa ccttttaccc tcggtattga actggaaatg
    28801 caggtggtta atccgccggg ctatgactta agccaggact cttcaatgct gattgacgcg
    28861 gttaaaaata agatcacggc cggagaggta aagcacgata tcaccgaaag tatgctggag
    28921 ctggcgacgg atgtttgccg tgatatcaac caggctgccg ggcaattttc agcgatgcag
    28981 aaagtcgtat tgcaggcagc cgcagaccat catctggaaa tttgcggcgg tggcacgcac
    29041 ccgtttcaga aatggcagcg tcaggaggta tgcgacaacg aacgctatca acgaacgctg
    29101 gaaaactttg gctatctcat ccagcaggcg accgtttttg gtcagcatgt ccatgttggc
    29161 tgtgccagtg gcgatgacgc catttatttg ctacacggct tgtcacggtt tgtgccgcac
    29221 tttatcgccc tttccgccgc gtcgccgtat atgcagggaa cggatacgcg ttttgcctcc
    29281 tcacgaccaa atattttttc cgcctttccc gataatggcc cgatgccgtg ggtcagtaac
    29341 tggcaacaat ttgaagccct gtttcgctgc ctgagttaca ccacgatgat cgacagcatt
    29401 aaagatctgc actgggatat tcgccccagt cctcattttg gcacggtgga ggttcgggtg
    29461 atggataccc cgttaaccct tagccacgcg gtaaatatgg cgggattaat tcaggccacc
    29521 gcccactggt tactgacgga acgcccgttt aaacatcagg aaaaagatta cctgctgtat
    29581 aaattcaacc gtttccaggc ctgccgctat gggctggaag gcgtcattac cgatccgcac
    29641 accggcgatc gtcgaccgct aacggaagac accttgcgat tgctggaaaa aatcgcccct
    29701 tccgcacata aaattggtgc atcgagcgca attgaagcac tgcatcgcca ggtcgtcagc
    29761 ggtctgaatg aagcgcagct gatgcgcgat ttcgtcgccg atggcggctc gctgattggg
    29821 ctggtgaaaa agcattgtga gatctgggcc ggtgactaag tcgggaattg ccttacagaa
    29881 gaatatccct gacaatggtg ttttaactta catttaacaa tgttatgaaa caccctttag
    29941 aaaccttgac caccgcagca ggcattttgc tgatggcttt cctctcttgc ctgctgcttc
    30001 ccgcccccgc attggggctg acgctggcac aaaaactggt gaccacgttc catctgatgg
    30061 atcttagtca gctttacact ttattgtttt gtctgtggtt tttagtgctg ggcgctattg
    30121 agtattttgt tctgcgcttt atctggcgac gctggttctc gctggcggat taaacgtcga
    30181 ttatcgccgt acttcgcggc atactttgct tattctcctt cgcctaaagg aacgtttatg
    30241 gataagcaat cactgcacga aacggcgaaa cgcctggccc ttgagttacc ctttgtcgag
    30301 ctttgctggc cttttggccc ggagttcgat gtttttaaaa ttggcggcaa gatttttatg
    30361 ctgtcgtcgg agctacgcgg cgtccccttt atcaatctga agtccgatcc acaaaaatcc
    30421 ctgttaaatc agcaaatata cccaagcatt aagccagggt atcacatgaa taaaaagcac
    30481 tggatttcgg tgtatcccgg cgaggaaatc tccgaagcgt tacttcgcga tctgatcaac
    30541 gattcgtgga atctggtggt tgatggtctg gctaaacgcg atcaaaaaag agtgcgtcca
    30601 ggctaaagcg gaaatctata gcgcattttt ctcgcttacc atttctcgtt gaaccttgta
    30661 atctgctggc acgcaaaatt actttcacat ggagtcttta tggatatcat ttctgtcgcc
    30721 ttaaagcgtc attccactaa ggcatttgat gccagcaaaa aacttacccc ggaacaggcc
    30781 gagcagatca aaactctcct gcaatacagc ccatccagca ccaactccca gccgtggcat
    30841 tttattgttg ccagcacgga agaaggtaaa gcgcgtgttg ccaaatccgc tgccggtaat
    30901 tatgtgttca acgaacgtaa aatacttgat gcctcgcacg tcgtggtgtt ctgtgcaaaa
    30961 accgcgatgg acgatgcctg gctgaagctg gttgttgacc aggaagatgc tgatggccgc
    31021 tttgccacgc cggaagcgaa agccgcgaac gataaaggtc gcaagttctt cgccgatatg
    31081 caccgtaaag atctgcatga tgatgcagag tggatggcaa aacaggttta tctcaacgtc
    31141 ggtaacttcc tgctcggcgt ggcggctctg ggtctggacg cggtacccat cgaaggtttt
    31201 gacgccgcca tcctcgatgc agaatttggt ctgaaagaga aaggctacac cagtctggtg
    31261 gttgttccgg taggtcatca cagcgttgaa gattttaacg ctacgctgcc gaaatctcgt
    31321 ctgccgcaaa acatcacctt aaccgaagtg taattctcct gacagccggg accccatccc
    31381 ggctcagatt gctgacaaag tgcgctttgt ttatgccgga tgcggcgtga ccgccttatc
    31441 ctgcctacaa gttcgtgcca attcattcag gtaggcatga taagcgtagc gcatcaggca
    31501 attttgtatt tgccatcagc cctcgcagga aatcccccct cagattctcc tgatttgcat
    31561 aaccctgttt cagccgtcat cataggctgc tgttgtataa aggagacgtt atgcaggatt
    31621 taatatccca ggttgaagat ttagcgggta ttgagatcga tcacaccacc tcgatggtga
    31681 tgattttcgg tattattttt ctgaccgccg tcgtggtgca tattattttg cattgggtgg
    31741 tactgcggac cttcgaaaaa cgtgccatcg ccagttcacg gctttggttg caaatcatta
    31801 cccagaataa actcttccac cgtttagctt ttaccctgca ggggattatc gtcaatattc
    31861 aggcggtatt ctggctgcaa aaaggcaccg aagcggcaga tattctgact acctgcgcgc
    31921 agttgtggat catgatgtat gcgctgcttt cagtcttctc gctgctggat gttattttga
    31981 atctggcgca gaaattcccg gcagcatctc agttaccgct gaaagggata tttcaaggga
    32041 ttaaactgat cggcgcgatt ctggtcggca ttttgatgat ctcgctgctg attggtcagt
    32101 cgccagcgat tctgatcagc ggtcttggtg caatggctgc cgtgctgatg ttggtattta
    32161 aagatccgat tcttggtctg gtggcaggta ttcagctttc cgcgaacgat atgctgaaac
    32221 tgggcgactg gctggagatg ccgaaatacg gcgcggatgg cgcggtgatc gatattgggt
    32281 taaccaccgt caaagtgcgt aactgggaca ataccattac cactattccc acctggtctc
    32341 tggtttctga ctcctttaaa aactggagcg ggatgtcagc atccggcggg cgacgtatta
    32401 agcgcagtat cagtattgat gtcaccagta ttcgttttct tgatgaagac gaaatgcaac
    32461 gtctgaataa agcgcatttg ttaaagcctt atttaaccag ccgccatcag gaaattaatg
    32521 agtggaatcg ccagcaaggt tctacggagt cggtattaaa cctgcgccga atgaccaata
    32581 ttggaacctt tcgtgcctat ctgaacgaat atctgcgtaa ccatccgcgg attcgtaaag
    32641 atatgacctt aatggtacgc caactggctc caggtgataa cggtttaccg cttgagatct
    32701 atgcgtttac caacaccgtg gtgtggctgg aatatgaaag cattcaggct gatatattcg
    32761 atcacatatt tgcgattgtc gaagagtttg gtctgcgact tcatcagtcg ccaaccggca
    32821 atgatattcg ctctctggcg ggtgcattta agcagtaatt aaaaaaaccg ctctcatcga
    32881 atggatgaga gcggacatcg ttttgtcgga tgtagcgtga atgccttatt tccgacgcag
    32941 cgttttaaat gccacaaaca ggaatacaat ccacaccggc agcaggatcg ctgacaagcg
    33001 catatcatct atcgtgcaca tcagcagcaa aatcatgccg aggaaggcaa tgcagagata
    33061 gtttccgaac ggatagagca gcgccttaaa ctgtgtttca cgcccctgac gtcgcatcgc
    33121 tgcacgaaaa cgcagatgcg ccagacagat cataatccag ttcaacagca gcgttgctac
    33181 caccagcgcc atcagcagac caaacgcttt ttgcggcagc agatagttga ttaacaccac
    33241 cagcgaagtg atcgctccgg aaagcatcag cgagttaatc ggcacgccgc gacggctgac
    33301 gcgagtcaaa aacttcggcg cattaccctg tacagaaagg ccaaacagca tgcggctgtt
    33361 agagtaaacc ccgctgttat acactgacag cgatgctacc agaatgacga agttcagcgc
    33421 agaagctacc acgttgctgt cgagattatg gaaaatcatc acaaacgggc tactgttgga
    33481 tttcacttcc acccacggat agagcgccag taaaaccacc agtgaaccga tgtaaaacag
    33541 caggatgcga tacaccacct gatttaccgc ttttggaatg cttttttccg gatcgcgcgc
    33601 ttcagcggca gtaatcccaa tcagctccag accgccgaag gagaacataa ttaccgccag
    33661 cgacaaaatc agcccattcc agccggtggc gaagaaacca ccgtagcgcc agaggttgtc
    33721 gatactggct ttctcgccgc cgtgaccaga aaacagcagc cacaggccaa agccgatcat
    33781 accgatgatt gccagcactt taatcagcgc aaaccagaac tcggtttcgc catataagcg
    33841 cacgttcacc aggttaacgg cgttgatgat aataaagaag gcggcagccc aaatccacgt
    33901 tggaacatcc gggaaccagt actgcatata gatgcccgca gcggtcagct ctgccattcc
    33961 caccagcacg aacatcaccc agtaattcca gccagagagg aagcccgcga acggtcccca
    34021 gtatttatag gcaaagtggg caaatgaacc ggataccggc tcctcaacca ccatttcgcc
    34081 aagctggcgc ataatcagga aagcgatgat cccggcgacg ccgtagccca gcaatacagc
    34141 cggacccgcc atctgaatcg ccgggccaat gccaagaaac agaccagtac caattgcgcc
    34201 acccagcgca atcagttgaa tatgacggtt atgtaatccg cgatgaagcg tcggctcttg
    34261 attcgacgca gtatcttccg atacggttga cgcgtttttc acgcatttcc cctgtgtgtc
    34321 ttttttgttg aggggcacct tttaacattt agtgcccatc gtcgcaagac acaatccaca
    34381 cggttaaacg gggtatcctg cttttatttc cgtacccgat gtcggtgcag ccacatcagc
    34441 ttatacgccg ccgggataat aaacagcgac agcaaaggcg cggtgatcat gccgccaatc
    34501 atcggcgcgg caatccggct catcacctct gaccctgctc ctgttcccca cagaatcggc
    34561 aacagaccag cgataatcac cgccaccgtc atcgctttcg ggcgcacgcg caggaccgcg
    34621 ccgtgataca acgcctcatc cagcttctgc tcgctgaatg tttgcggatt attcaacgac
    34681 ggctcggcct ctatggcgtg acgtaaatac atcagcatca ccacgccaaa ttcggcggcg
    34741 accccggcaa gggcaataaa accggtgccc gtcgccacag aaagatgaaa gcccatccac
    34801 cacagcaacc agatgccgcc caccagcgca aacggtacgc tgctgataat cagcaacgct
    34861 tcgcccaccc gacggaatgc cagatacaac agcacgaaga tgatcatcag tgtcatcggc
    34921 accatgagtt tcagcttatg gttggcgcgc tccagtagct cgaactgccc ggagaatgcc
    34981 acgctggtgc ccggttttaa ctgcactttt tcagctatcg ctttttgcaa atcgtgaacc
    35041 accgacacca tgtcacgatc gcgggcatcg atataaatcc agctcgtcgg gcgcgcattc
    35101 tcggttttca gcattgacgg tccggtagag actttaacgt cggccacgtc tgccagggtg
    35161 atttgctgct tcatcggcgt caggatcggc aactggcgca gtgcctgcgg actatcgcgc
    35221 cagctttgcg gataacgcag attaattgga taacgggcaa tcccttccac cgtttcgcca
    35281 accatcgccc cgcccaccgc agaagtcaca aacaactgca catccgccac cgtcataccg
    35341 taacgcgcgg ctttttcacg gttaatctca acgttgatat agcgcccacc ttccagacgc
    35401 tcggcaagcg cagaagctac gcctggcacc gttcgcgcca cttcttcaat ttgctccgcc
    35461 atcgcgtcga tatccgccag cacagtgccg gaaactttaa tgccgatggg gcttttaatg
    35521 ccggttgaga gcatatcgat acggttacga attggcggca cccacagatt cgccagcccc
    35581 ggcagacgca cggtgttatc cagttcctca atgattttgt ccattgtcat gcctggccgc
    35641 cactgatcct gcggcttaag ctggatggtc gtttctacca tctccagcgg agcagaatcg
    35701 gtggcggttt ccgctttccc ggttttgcca aatacccgcg ccacttcagg tacgctcata
    35761 atcagcttgt cggttttttg cagcatactc gccgcctctg ctgcggaaat ccccggcagc
    35821 gtcgatggca tatacaacaa gtcgccttca ttgatctgcg gtaaaaattc cccgccaact
    35881 ttattgagcg gccagagaac cgtcagcacc gaaagcgctg ccaccaacag cgtggttttc
    35941 ggccagtgca gtactttcag caatagagga tgataaacac gaatcaaaaa gcgattgagc
    36001 gggttactgc tttccggcgg aattttgcca cggatccagt aacccatcag gatagggatc
    36061 actacgatcg ccagcagcgc cgcacccgcc atcgcatacg ttttggtgaa cgccagcggg
    36121 ccaaacagac gcccttcctg cccttccagg gtgaagatcg ggataaacga cagtgtgata
    36181 atcagcagac tgataaacag cgccggtccc acttcaacag acgcatcggt aatcacctgc
    36241 cagcgcgttt tattatccag cgtggtgtca ggatgctggt gctgccactc ttccagccgt
    36301 ttatgcgcat tctcgatcat gacgatagcg gcatcgacca tcgccccgac ggcaatcgca
    36361 ataccgccca gcgacataat attggcattc agcccctgga agtgcatgac aataaaagca
    36421 atacacaacc ccagcggcaa cgaaataatc gccaccagcg ccgagcgcac atgccagaga
    36481 aacagcgcac agactaccgc caccacaata aactcttcca gcaacttgcc gctgagattg
    36541 tcgatggccc ggtcaatgag ctggctgcga tcgtatgttg taactatctc cacgccttcc
    36601 ggcagactgc ttttcagcgt ttctagtttg tccttcacgg cggcgatcac ttcacgggcg
    36661 tttttgccgg agcgtaggat caccaccccg cccgcaactt cgccttcgcc gttcagttcg
    36721 gcaatgcccc ggcgcatctc cgggccaacc tggatcttcg caacatcacg cagataaacg
    36781 ggcacgccat tttcactggc ttttaaaacg atgtgattaa agtcgtcgag cgtttgtaga
    36841 tagccgctgg cgcgcaccat atattctgct tccgccagtt cgattgacga accgcccgct
    36901 tcctggtttg aagcatccag cgcgctttta acttcggcga gactgatgcc atactgcgcc
    36961 aggcgctggg gatcgataac cacctgatac tctttcacca cgccgcccac cgacgccact
    37021 tccgcaacgt cagggatggt ttttagctca tatttgagaa accagtcctg caatgagcgt
    37081 aaatcggcca gatcgtgctt accgctgcga tccaccagtg catattcata gatccagcca
    37141 acacccgtgg catctggccc cagctcggcg ctgactcccg caggcagctt accctgtacc
    37201 tggttgaggt actccagcac ccgcgagcgc gcccagtacg gatcggtgcc atcttcgaaa
    37261 atgacataca cataagagtc gccaaactgc gagaaaccgc gcacagtctt cgcgccaggt
    37321 accgacaaca tggtggtagt tagcggataa gtcacctgat tttcaacgat ttgcggtgcc
    37381 tgcccgggat agctggtttt aataatcacc tgcacatcgg agagatccgg caacgcatcc
    37441 actggcgtgt taatgatggt ccaggtgccc cagatactca gaaataacgc gcccatcagc
    37501 accagaaaac ggttcgccac cgagcgacga ataatccatt caatcattgg ttattccctc
    37561 aatgcgcatg ggtagcactt tcagagcgca tccgctccag tgcgccagaa atattggctt
    37621 cggaatcaat caggaacagg ccgctggaaa ccaccttttc accttccgcc agaccagagc
    37681 gtaacgcggt gacaccttgc gatgcctgga aaacggcaac gcgtttcggt acaaagcgcc
    37741 cgtcggcatc aacggtaatc acccgctgtt cgttgccggt atcaatcagc gcttgtgacg
    37801 gaatgagcag catcggttcg ctggcggtat tgagttgcaa ccaggcgttc attcccggtt
    37861 ttagcgcctc gtcggcgttg tcgacttcca gacgcagctg cagcgtgcgg gtcgcggaat
    37921 ccacgccagg tagtagcgtc catttgcgga tggtgagtgt tttatcaggt cgcgccggaa
    37981 cggtgagcgt aaactgcgag gcatctttca ccagccaggc gatggactcc gggatcgcag
    38041 cagtgaccca caccgggtcc ataccctgaa ttttcgcgac cacgttatct ttggcgatat
    38101 tcattcccgc gcgcagatca aacgcggtga tcacgccatc aatgggtgct ttgagcgtaa
    38161 agcgagtctg gattttttgc gtggcgatca ggcggcgaat atccgcctcc ggcattcccg
    38221 ccagtcgcag ccgctcaaga atgccttcag tctgggtcgc cgtaccgccg gtttcgcgca
    38281 gcagtaaata ctcactctgc gcttccaccc agtcgggaat ggtcaggtcg agaagcggtg
    38341 tgcccttttg cactttatcg cccacggtaa gcggatacac cttgtcgata aacccggcag
    38401 cgcgagcctg cacaatggca tactgatact cgttgtaact gacattcgcc gggaaactct
    38461 gggcaaaagt cagcggtccg cgcgtgacgg tagccgtttt cacccccaga ttctgcgtct
    38521 gagtcgggtc aatgcgcaca ccagacgcag aactctcttc atcggcatat ttcggcacca
    38581 gatccatatc cataaacggc gatttacctg gtttatcgaa ccgcgtattg ggatacatcg
    38641 ggtcgtacca gaataagatt ttacgttctg cagtcgacgt tttttctgcg ggcggttcag
    38701 cccggtcaaa ccaggtaaaa cctgccgcag aaataatacc gcccgcgatc atgctgccga
    38761 taataagcgc gatttttttc atctcattaa acctgggtta ctggctgact ttaatatcct
    38821 gtaataaaga aaggttgccc tgctggacaa aattaaacgc cactttgtcg ccagttttaa
    38881 tttcactcat tttcgtctgc ggagtgatgg taaagcgcat agtcatctcc ggccagttca
    38941 cggcagcaat cggatcgtga tggatggtga tttttttgct ttccagatca acacctttta
    39001 ccacgccggt ggcactaatg acctgtggtt gtgcttcgct catggtttca tgatgatgtt
    39061 cgttagcctg gacattaaag ccaataacgg taaacagact gaacattgcg acttgcagtg
    39121 cttttttcat ttactctctc ctggagttaa aaattattgc tgccaaccgc caccaagtgc
    39181 ggtatataaa gaaatttcgt tgacctgacg ggcataattc aggtcaagta aggtttgtcg
    39241 ggttgcaaat aaagaacgct cggcatccag cacttccaga taacttaccg cgccgtgctg
    39301 atataatgcc cgcgcccgtt gcaaagtaat ttgcagcgac gccagataac gctgctgggc
    39361 gctgatttga tcgttcaggc tttgacgtaa tgccagcgca tctgccactt ctttaaaggc
    39421 gttctggatt ttctgttcat aattcactac cgactgctgc tggcgaattt cggcgatatc
    39481 cagattggcc tggttgcgtc cggcattaaa aatggggatc tcaattttgg gtataaaatt
    39541 ccacatcccg ctgctggcgt taaataatga cgacagatcg ctgctggcgg ttgatattcc
    39601 gctggtcagg ctgatagacg ggaaaaatgc cgcgcgtgca gcaccaatat tggcattagc
    39661 cgccattaac gcatgttcag cttccataat atccgggcgc tgcaataaga tttgcgacga
    39721 caagcccgcc ggtaatttaa cgctttgcag actgccgccg tttactgtct gcgcttgcgg
    39781 cagcttgccg tagcttccca ataacagttg caatgcatta ttcgcctgcg ccagttcccc
    39841 ctgacgttta gcgatgtcgc tgcgggtact ttcaatcacc ccgcgagcct gttccagcgc
    39901 cagaacatta ctgctacctg tcaacagttg tttttcgaca aacgcatatg actgctgata
    39961 attacgcagc gtttcttcgg ctatttgcaa ttgcgcatac gccagctgct gattgaaata
    40021 gctttgcgcg acattagaaa ccagcagaat atgcaccgcg cgctgagctt cctcagtggc
    40081 taaataattt tgtcgctcgg cttcgctcat gttctttaag cgcccgaaaa aatcgaggtc
    40141 aaagctggcg ttaaggccag tagaaaactc ccgcgtcgtg gctgaatcgc ctttaagatt
    40201 gccgctccag ctgccgctgc cctcaccatt gagctgtggg tagcggtcgg catcagtcag
    40261 acgatattgc gcccgtgcct cctgcacttt cagcgtcgcc atgcgcaaat cccggttatt
    40321 ctccagcgcc tcgctaatca gcgtcttcac ctgattatca acaaaaaagg tgcgccagcc
    40381 cgcgttctga tagttatctg ctgcgttaac caggccgttc tggctgagtg agaactgctg
    40441 aggaacgggc attgccggac gctgataatc cggtgccagt gaacaaccgg ttagcgcaag
    40501 ggccacacaa aatggcagaa gtttacaagg agacataggc tcataatttc tggtgatttt
    40561 ataccgccaa ctttactcgc caggctctga ttttccggtg acaggaaaat gacaaaattg
    40621 tcattttgcc aataagcgat tgccatctga tcccgctact ctagaattgc ccgggcaaca
    40681 tgcggaggaa atatgaaact gttgattgtc gaagatgaaa agaaaaccgg agaatacttg
    40741 accaaagggt taaccgaagc cggttttgtg gtcgatttgg ccgacaacgg gctgaatggc
    40801 taccatctgg cgatgaccgg tgattatgat ctgataatcc tcgatattat gctgccggac
    40861 gtgaacggct gggatatcgt gcgcatgtta cgctccgcca ataaagggat gccgattctg
    40921 ttgcttaccg cgcttggcac cattgaacat cgcgtcaagg ggctggagtt gggggcagat
    40981 gactacctgg tgaaaccatt cgcttttgct gaactactgg cgcgggtgcg cacattactg
    41041 cggcgcgggg cggcggtgat tatcgaaagt cagtttcagg ttgccgattt gatggtcgat
    41101 ctcgtcagcc gcaaagtcac ccgcagcggc acgcgcatca ctttgaccag taaagagttt
    41161 actctgctgg agttttttct ccgccatcag ggcgaagtgc tgccccgctc gcttatcgcc
    41221 tcgcaggtat gggacatgaa ttttgacagc gacactaacg ccattgatgt ggcggtgaag
    41281 cggctgcgcg gcaaaatcga caacgacttt gagccgaagc taattcagac cgtgcgcggc
    41341 gtgggttaca tgcttgaggt gccggatggt cagtaagcca tttcagcgcc cgttttcgct
    41401 ggcaacccgc ctgacctttt ttatcagcct ggccaccatc gcggcgtttt tcgcctttgc
    41461 atggatcatg atccactcag taaaagtgca ttttgccgag caggatatta atgatttaaa
    41521 agagattagc gccacccttg aacgcgtact aaatcaccct gacgaaacgc aagcccgacg
    41581 cttaatgacg ctggaagata tcgtcagtgg ttattccaac gtgttgattt ccctggcaga
    41641 tagccacggt aaaacggtgt atcactcccc cggtgcgccg gatatccgcg agtttacgcg
    41701 tgacgccata cccgataaag acgctcaggg tggcgaggtg tatctccttt ccggcccgac
    41761 gatgatgatg ccaggccacg gtcacgggca tatggaacac agcaactggc ggatgatcag
    41821 tttgccagtt ggcccactgg ttgacggcaa accgatttat acgctctaca tcgcgctttc
    41881 gatcgatttt catcttcatt acataaatga tttgatgaat aaacttgtaa tgaccgcatc
    41941 gatcatcagc atcctgattg tctttatcgt gctattggcg gtacataaag gtcacgcgcc
    42001 gattcgcagc gtcagccgtc aaatccagaa tattacctcg aaagatctcg acgttcgcct
    42061 cgacccgcag accgtgccga ttgagctgga acagctggta ctgtcgttca accatatgat
    42121 cgagcgtatt gaggatgtct ttacccgcca gtccaatttc tcagcggata tcgctcacga
    42181 aattcgtacg ccgattacca atctcatcac gcaaacggaa atcgccctca gccagtctcg
    42241 cagccagaag gagctggaag atgtgctcta ctctaatctc gaagagctga cgcgaatggc
    42301 gaaaatggtc agcgatatgc tgtttctcgc ccaggccgat aacaaccagc taatccccga
    42361 aaagaaaatg ctcaacctgg cggatgaaat cggcaaagtg ttcgattttt tcgaggcgtt
    42421 agcggaagat cgcggcgtgg agttgcggtt tgttggcgac aaatgtcagg ttgcgggcga
    42481 tccgctgatg ctgcgtcggg cgttaagcaa cctgctttct aatgccctgc gttatacgcc
    42541 acccagtgag gctattgtag tgcgctgcca gacggtcgat caccaggtgc aagttatcgt
    42601 cgaaaacccc ggtactccca ttgcgcccga gcacttaccg cgattgtttg accgtttcta
    42661 ccgcgttgac ccttcccgcc agcgaaaagg cgaaggtagc ggcattgggc tggcgatagt
    42721 gaaatcgatt gttgtcgcac ataaaggcac ggttgcagta acgtcagatg cgcgggggac
    42781 aaggtttgtg attgtgttgc cggagagggg gtgattatta agttcaaatt cagggcgagg
    42841 gtttacagcg tttttcgcta aatttccatc attata
//
LOCUS       DAZFKF010000035        41077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFKF010000035 DAZFKF010000000
VERSION     DAZFKF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.035"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2117
                     /locus_tag="ACHKA1_002291"
     CDS             <1..2117
                     /locus_tag="ACHKA1_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFO5679936.1"
                     /translation="ACGVYPQDLKDREIISTFHAEKIINDLLKNDYKISLFPDTTYRE
                     LNKAAQRSITAPDRIGEGKTWIYQRDTMNEREDNKGVHQYGPAEHFTHIISDKPSPKD
                     KYVAYAINIPDYELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQ
                     CEIITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEE
                     KGYWFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKND
                     LDYPSADIRAKISKDIEGGIPGYWQGQVLIPFIIDPVTKERKQIHPEDIHLTAKDFEA
                     SKDNISKDEWENLHALKEKRLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAA
                     KHSKHEQIERSITPPLKNYNERLHNTNLFNPKYHEKAVMNYMEYLVTSQLDEQQMSSL
                     LYERDDLSAIDFSLLMKTIKAFSFGGDLQTLASKPGSTISSIPSKRRILININHDFPN
                     NGNLFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNDDGSTRVGGE
                     AWLNKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDG
                     LVIINKGNEEVEGTWPNKLQPGKYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNI
                     NIPGRSALLLGKTGEPPNYLYL"
     gene            2452..3429
                     /gene="glaH"
                     /locus_tag="ACHKA1_002292"
     CDS             2452..3429
                     /gene="glaH"
                     /locus_tag="ACHKA1_002292"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO5679937.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLTPSAQSPRLLELTFTEQTTKQ
                     FLAQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3448..4716
                     /gene="lhgO"
                     /locus_tag="ACHKA1_002293"
     CDS             3448..4716
                     /gene="lhgO"
                     /locus_tag="ACHKA1_002293"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO5679938.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARNVDALHAAFNQ"
     gene            4739..6187
                     /gene="gabD"
                     /locus_tag="ACHKA1_002294"
     CDS             4739..6187
                     /gene="gabD"
                     /locus_tag="ACHKA1_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO5679939.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGD
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            6201..7481
                     /gene="gabT"
                     /locus_tag="ACHKA1_002295"
     CDS             6201..7481
                     /gene="gabT"
                     /locus_tag="ACHKA1_002295"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO5679940.1"
                     /translation="MSSNKELMQRRSQAVPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHTEIGDVRGLGAMIAIELFEDGDHSKPNAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIIGQCFAEAKQ"
     gene            7899..9299
                     /gene="gabP"
                     /locus_tag="ACHKA1_002296"
     CDS             7899..9299
                     /gene="gabP"
                     /locus_tag="ACHKA1_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO5679941.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9320..9982
                     /gene="csiR"
                     /locus_tag="ACHKA1_002297"
     CDS             9320..9982
                     /gene="csiR"
                     /locus_tag="ACHKA1_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO5679942.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMNLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEARDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRQTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMCQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9983..10432)
                     /gene="kbp"
                     /locus_tag="ACHKA1_002298"
     CDS             complement(9983..10432)
                     /gene="kbp"
                     /locus_tag="ACHKA1_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO5679943.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10516..10674)
                     /locus_tag="ACHKA1_002299"
     CDS             complement(10516..10674)
                     /locus_tag="ACHKA1_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO5679944.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10857..11156
                     /locus_tag="ACHKA1_002300"
     CDS             10857..11156
                     /locus_tag="ACHKA1_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO5679945.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            11166..11684
                     /gene="ygaP"
                     /locus_tag="ACHKA1_002301"
     CDS             11166..11684
                     /gene="ygaP"
                     /locus_tag="ACHKA1_002301"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO5679946.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQ"
     gene            complement(11737..12141)
                     /gene="stpA"
                     /locus_tag="ACHKA1_002302"
     CDS             complement(11737..12141)
                     /gene="stpA"
                     /locus_tag="ACHKA1_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO5679947.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            12809..13258
                     /gene="alaE"
                     /locus_tag="ACHKA1_002303"
     CDS             12809..13258
                     /gene="alaE"
                     /locus_tag="ACHKA1_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO5679948.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKISPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
     gene            complement(13295..13639)
                     /locus_tag="ACHKA1_002304"
     CDS             complement(13295..13639)
                     /locus_tag="ACHKA1_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO5679949.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            13791..14120
                     /locus_tag="ACHKA1_002305"
     CDS             13791..14120
                     /locus_tag="ACHKA1_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO5679950.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(14270..15604)
                     /locus_tag="ACHKA1_002306"
     CDS             complement(14270..15604)
                     /locus_tag="ACHKA1_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO5679951.1"
                     /translation="MPRYQDIARQLKTAIEQGELKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLVRRGQAGTFVSEQIHPQQSAVDVVAFAGENQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASMALILHALAQPGDGMWMEDPGFPLIRPIVTRHGVEILPVPVDDNGLDVTSGIQN
                     YPDARFALITPAHQSPLGVALSLARRYQILEWAERSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVEQISQFRQQASLEPCAVPVLWQNTL
                     ADFIREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIKLAGNDVTF
                     VHKANAAGLAVQALSDWRIRSSEDGGLLLSFTNIVSESMARQVAQQLRSALN"
     gene            15692..16123
                     /locus_tag="ACHKA1_002307"
     CDS             15692..16123
                     /locus_tag="ACHKA1_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO5679952.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELIYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTDIAKT
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            16332..16577
                     /gene="nrdH"
                     /locus_tag="ACHKA1_002308"
     CDS             16332..16577
                     /gene="nrdH"
                     /locus_tag="ACHKA1_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO5679953.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            16574..16984
                     /gene="nrdI"
                     /locus_tag="ACHKA1_002309"
     CDS             16574..16984
                     /gene="nrdI"
                     /locus_tag="ACHKA1_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO5679954.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            16957..19101
                     /gene="nrdE"
                     /locus_tag="ACHKA1_002310"
     CDS             16957..19101
                     /gene="nrdE"
                     /locus_tag="ACHKA1_002310"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO5679955.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFDHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VVDERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYNENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            19111..20070
                     /gene="nrdF"
                     /locus_tag="ACHKA1_002311"
     CDS             19111..20070
                     /gene="nrdF"
                     /locus_tag="ACHKA1_002311"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO5679956.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKRLEKISEAQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            20426..21628
                     /gene="proV"
                     /locus_tag="ACHKA1_002312"
     CDS             20426..21628
                     /gene="proV"
                     /locus_tag="ACHKA1_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO5679957.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKAIDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            21621..22685
                     /gene="proW"
                     /locus_tag="ACHKA1_002313"
     CDS             21621..22685
                     /gene="proW"
                     /locus_tag="ACHKA1_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO5679958.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            22743..23735
                     /gene="proX"
                     /locus_tag="ACHKA1_002314"
     CDS             22743..23735
                     /gene="proX"
                     /locus_tag="ACHKA1_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO5679959.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            23927..25111
                     /locus_tag="ACHKA1_002315"
     CDS             23927..25111
                     /locus_tag="ACHKA1_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5679960.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAACGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYNDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            25235..25972
                     /gene="ygaZ"
                     /locus_tag="ACHKA1_002316"
     CDS             25235..25972
                     /gene="ygaZ"
                     /locus_tag="ACHKA1_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO5679961.1"
                     /translation="MESPTTQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            25962..26297
                     /gene="ygaH"
                     /locus_tag="ACHKA1_002317"
     CDS             25962..26297
                     /gene="ygaH"
                     /locus_tag="ACHKA1_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO5679962.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            26388..26918
                     /gene="emrR"
                     /locus_tag="ACHKA1_002318"
     CDS             26388..26918
                     /gene="emrR"
                     /locus_tag="ACHKA1_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO5679963.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            27045..28217
                     /gene="emrA"
                     /locus_tag="ACHKA1_002319"
     CDS             27045..28217
                     /gene="emrA"
                     /locus_tag="ACHKA1_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO5679964.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQTFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            28234..29772
                     /gene="emrB"
                     /locus_tag="ACHKA1_002320"
     CDS             28234..29772
                     /gene="emrB"
                     /locus_tag="ACHKA1_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO5679965.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(29836..30351)
                     /gene="luxS"
                     /locus_tag="ACHKA1_002321"
     CDS             complement(29836..30351)
                     /gene="luxS"
                     /locus_tag="ACHKA1_002321"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO5679966.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(30501..32057)
                     /gene="gshA"
                     /locus_tag="ACHKA1_002322"
     CDS             complement(30501..32057)
                     /gene="gshA"
                     /locus_tag="ACHKA1_002322"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO5679967.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(32130..32558)
                     /locus_tag="ACHKA1_002323"
     CDS             complement(32130..32558)
                     /locus_tag="ACHKA1_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO5679968.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVATLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(32555..33121)
                     /gene="yqaB"
                     /locus_tag="ACHKA1_002324"
     CDS             complement(32555..33121)
                     /gene="yqaB"
                     /locus_tag="ACHKA1_002324"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO5679969.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(33404..33480)
                     /locus_tag="ACHKA1_002325"
     tRNA            complement(33404..33480)
                     /locus_tag="ACHKA1_002325"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33444..33446),aa:Arg,seq:acg)
     gene            complement(33680..33756)
                     /locus_tag="ACHKA1_002326"
     tRNA            complement(33680..33756)
                     /locus_tag="ACHKA1_002326"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33720..33722),aa:Arg,seq:acg)
     gene            complement(33760..33852)
                     /locus_tag="ACHKA1_002327"
     tRNA            complement(33760..33852)
                     /locus_tag="ACHKA1_002327"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33816..33818),aa:Ser,seq:gct)
     gene            complement(34168..34353)
                     /gene="csrA"
                     /locus_tag="ACHKA1_002328"
     CDS             complement(34168..34353)
                     /gene="csrA"
                     /locus_tag="ACHKA1_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO5679970.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(34588..37218)
                     /gene="alaS"
                     /locus_tag="ACHKA1_002329"
     CDS             complement(34588..37218)
                     /gene="alaS"
                     /locus_tag="ACHKA1_002329"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO5679971.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(37346..37846)
                     /gene="recX"
                     /locus_tag="ACHKA1_002330"
     CDS             complement(37346..37846)
                     /gene="recX"
                     /locus_tag="ACHKA1_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO5679972.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATEEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLSTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(38083..39144)
                     /gene="recA"
                     /locus_tag="ACHKA1_002331"
     CDS             complement(38083..39144)
                     /gene="recA"
                     /locus_tag="ACHKA1_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO5679973.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(39224..39721)
                     /gene="pncC"
                     /locus_tag="ACHKA1_002332"
     CDS             complement(39224..39721)
                     /gene="pncC"
                     /locus_tag="ACHKA1_002332"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO5679974.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(39866..40951)
                     /gene="mltB"
                     /locus_tag="ACHKA1_002333"
     CDS             complement(39866..40951)
                     /gene="mltB"
                     /locus_tag="ACHKA1_002333"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO5679975.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         9904 a        10701 c        10355 g        10117 t
ORIGIN      
        1 atgcttgtgg agtttatcct caggatttaa aagacagaga aattatatca acttttcacg
       61 cagaaaaaat cattaatgat ctgttaaaaa acgattataa aatttccctt tttccagata
      121 caacttaccg tgaactgaat aaagctgcac agcgtagtat tacagcgcca gacaggatag
      181 gagaaggaaa aacatggatt tatcaaagag atacaatgaa tgaaagagaa gataacaagg
      241 gggttcacca gtatggtcct gctgaacatt tcacccacat tatatctgac aaaccctccc
      301 caaaagataa atatgttgca tatgctatta acattcctga ctatgagctg gcagccgatg
      361 tatataatat taacgtgacg tcaccttccg gacagcaaga aacatttaaa atactaatca
      421 atccagaaca tctacggcaa acacttgagc gtaaatctct tactgctgtt cagaaatcac
      481 aatgtgaaat catcaccccc aaaaaacctg gcgaagcgat tcttcatgct tttaatgcca
      541 cctaccagca aatcagagaa aatatgtctg aatttgcacg ttgccattat gggtatatac
      601 aaatccctcc agtgacaact ttccgtgccg acggaccaga aactcccgaa gaagaaaaag
      661 gttactggtt tcacgcttat caacccgaag atctttgtac catccacaat ccaatgggag
      721 atttgcagga ttttatcgca ttggttaaag atgctaaaaa atttggtatc gatatcattc
      781 ctgattatac ctttaacttt atgggaatcg gggggagtgg taaaaatgac ctggattatc
      841 cctctgctga tatacgagcg aagatcagta aagatataga aggtggtatc cctggctatt
      901 ggcaaggtca ggttttgatt ccatttatta tcgatccagt aacaaaagaa cgtaaacaaa
      961 tccatccaga agatatacat ctcactgcaa aagacttcga agctagtaaa gataatattt
     1021 ctaaggatga atgggaaaac ctccatgcat taaaagaaaa gcgtttaaat ggaatgccta
     1081 aaacaacccc caaaagtgac caggttatta tgctgcaaaa tcaatacgtt cgtgaaatgc
     1141 gaaaatatgg cgtacgaggt ttacgttatg atgcggcaaa acactcaaaa catgaacaaa
     1201 tagaaagatc aataacccca ccgcttaaaa attataatga gcgattacac aatactaact
     1261 tatttaaccc aaaatatcat gaaaaagccg ttatgaatta catggaatat ctggtaacta
     1321 gtcagttgga tgaacaacaa atgtcatcgc tgctttatga aagagatgat ttaagcgcca
     1381 ttgatttttc attgctcatg aagacgataa aagctttttc atttggtggc gatctccaaa
     1441 cccttgcatc aaaaccgggt tcaacaatct caagcatccc atcaaaaaga cggatattga
     1501 ttaacattaa ccacgatttt cctaacaatg gcaatctttt caatgacttt ctatttaacc
     1561 atcaacaaga tgaacaatta gcaatggcat atatggccgc tctcccgttc agcaggcctt
     1621 tagtttactg ggatggccaa gtattaaaat caacgactga aattaaaaat gatgatgggt
     1681 cgacgcgtgt cggcggtgag gcgtggctta ataaaggttg ctctacctat cagcagctct
     1741 acaatgaatt ccacgcatta tatatagata aagcaggaat atggagcgca tttgagggtg
     1801 tatttgcaac taaaaacgtt ctggccttta gtcgtgggga ttctgtgaac attaatcact
     1861 ctcctcatga tggactagtt ataataaata aaggaaacga agaagttgaa ggtacctggc
     1921 ctaacaaatt gcaacctgga aaatataaaa acatggggag taatagcgtt aacattatta
     1981 ttaataatac ccgaaaaatt atcccccctg gtaaagtatt tacgcttaga ggcggaactc
     2041 taaatatcaa tattcctggg cgtagcgctc ttcttttagg gaaaacagga gaaccgccga
     2101 actatctcta tttgtaattt aatgttatat ctgccccgct aaaacggggc agataatatg
     2161 tttagtttac taacggtcat tttgcattga agccatttac tgttttttat cgaccagata
     2221 atctgttctc tgatggtaac tccccctaac ctgttgcttt agttattcat ttcctgtctc
     2281 actttgcctt aataccctac gttaaatgtt actaatttgt tgtttttgat cacaataaga
     2341 aaacaatatg tcgcttttgt gcgcattttt cagaaatgta gatattttta gattatggct
     2401 acgaaatgag catcacgcac tctccctgaa acctcaagag gatcgcttct gatgaatgca
     2461 ctgaccgccg tacaaaacaa cgctgtcgat tcaggccagg actatagcgg attcaccctc
     2521 accccatcgg cgcaatcccc gcgtctgctg gaactcacct ttaccgaaca gacgaccaaa
     2581 cagttcctcg cgcaggttgc cgagtggccc gtgcaggcgc tggagtacaa atcgtttctg
     2641 cgttttcggg taggcaaaat tcttgatgat ctgtgtgcga atcagctgca accgttgctg
     2701 ttgaagaccc tgctaaaccg cgctgaaggt gcgttgttga tcaatgcggt gggggtcgat
     2761 gatgtgaagc aagcggatga gatggtgaag ctggcaacgg cggtggcgca tctgattggt
     2821 cgctccaatt tcgacgcgat gagcggtcag tattacgcgc gttttgtagt gaaaaatgtc
     2881 gataactcag acagctatct gcgtcagccg caccgcgtga tggagctgca caacgacggc
     2941 acctacgtgg aagagatcac cgactatgtg ctgatgatga aaatcgacga gcaaaacatg
     3001 cagggcggaa attcgttgct gctgcatctc gatgactggg aacatctcga ccactatttc
     3061 cgccacccgc tggcgcgccg cccaatgcgc tttgccgcgc cgccgagtaa aaacgtcagc
     3121 aaagatgttt tccatccggt gttcgacgtt gatcaacagg gtcgcccggt gatgcgttat
     3181 atcgaccaat tcgtccagcc aaaagatttt gaagaaggcg tgtggttgag cgagctttcc
     3241 gatgccattg aaaccagcaa aggcattctt tctgtgcccg ttcccgttgg caaattcctg
     3301 ttgattaaca acctgttctg gctgcacggt cgcgaccgct ttactccgca cccggatctg
     3361 cgccgtgaac tgatgcgtca gcgtggctat ttcgcttacg ccactcacca ctaccagacg
     3421 catcagtagc gcaaaggaat tgagcggatg tatgattttg tgattattgg cggtggcatc
     3481 atcggcatgt cgaccgccat gcaactgatt gatgtctacc cggacgcccg cattgcgttg
     3541 ctggaaaaag agtccggccc ggcctgtcac cagacggggc acaacagcgg cgtgatccat
     3601 gccggggtct attacacgcc cggcagcctg aaggcacagt tttgcctggc ggggaaccgc
     3661 gccaccaaag ccttttgcga tcaaaacggc attcgctacg acaactgcgg caagatgctg
     3721 gtcgccacgt ccgatctcga aatggaacgg atgcgcgcgt tgtgggaacg gacggcggcg
     3781 aacggcatcg agcgcgagtg gttaaacgcc gatgaactgc gcgagcgcga accgaatatc
     3841 accgggcttg gcggtatttt tgtgccgtcc agcggcattg tcagctaccg cgaagtcacg
     3901 gcggcgatgg caaaaatctt ccaggccaga ggcggcgaga ttatctataa cgccgaagtc
     3961 agcggcctta gtgagcataa aaacggcgtg gtgatacgta cccgtcaggg tggcgaatac
     4021 gaagcgtcaa cgctgattag ctgttccggg ctgatggctg accggctggt gaaaatgctc
     4081 ggcctcgaac cgggctttat tatctgcccg ttccgtggcg agtatttccg cctggcgccg
     4141 gagcataacc agattgttaa ccacctgatt taccccatcc ccgacccggc aatgccgttt
     4201 ttaggcgttc atctcacccg catgatcgac ggtagcgtca ccgttgggcc aaacgcagtg
     4261 ctggcgttca aacgcgaagg ctatcgcaag cgcgacttct cgtttagcga cacgctggag
     4321 attttaggct cgtcggggat tcgccgggtg ctgcaaaacc atctacgctc aggactgggc
     4381 gagatgaaaa actcgctgtg caaaagcggc tatctgcggc tggtgcaaaa gtattgtccc
     4441 cggctttcgt taagcgatct ccagccctgg cccgccggtg tgcgggcgca ggcggtatcg
     4501 ccggacggca agctgattga cgattttctg tttgtcacca ccccgcgcac gatccacacc
     4561 tgcaatgcgc cctccccggc agcgacatca gcaattccta ttggtgcgca tattgtcagc
     4621 aaggtacaaa cgctgttggc aagccagagt aaccccggac gcacgctgcg agcggcacgt
     4681 aatgtggatg ccttacacgc cgcatttaat caataacctt tgaaaacagg atgtagcgat
     4741 gaaactgaac gacagcaact tattccgcca acaggcgttg attaacgggg aatggctgga
     4801 cgccaacaat ggtgaagtca tcgacgtcac caatccggcg aacggcgaca agctgggtag
     4861 cgtgccgaaa atgggcgcag atgaaacccg cgccgctatc gacgccgcca accgcgccct
     4921 gcccgcatgg cgcgcgctca ccgccaaaga acgcgccaac attctgcgca actggttcaa
     4981 tttgatgatg gagcatcagg acgatttagc gcgcctgatg accctcgaac agggtaaacc
     5041 gctggctgaa gccaaaggcg aaatcagcta cgccgcctct tttattgagt ggtttgccga
     5101 agaaggcaaa cgcatttatg gcgacaccat tcccggtcat caggccgata agcgcctgat
     5161 tgtgatcaag cagccgattg gcgttaccgc ggctatcacg ccgtggaact tcccggcggc
     5221 gatgattacc cgtaaagccg gcccggcgct ggcggcaggc tgcaccatgg tactgaagcc
     5281 cgccagtcag acgccgttct ctgcgctggc gctggcggag ctggcgatcc gcgcgggcat
     5341 tccggctggc gtgtttaacg tggtcaccgg ttcggcgggc gcggtgggtg acgaactgac
     5401 cagcaacccg ctggtacgca aactgtcgtt taccggttcg accgaaattg gccgccagtt
     5461 aatggaacag tgcgcgaaag acatcaaaaa agtgtcgctg gagctgggcg gcaacgcgcc
     5521 gtttatcgtc tttgacgatg ccgatctcga taaagccgtg gaaggcgcac tggcctcgaa
     5581 attccgcaac gccgggcaaa cctgcgtctg cgccaaccgt ttatacgtgc aggacggcgt
     5641 gtatgaccgc tttgccgaaa aattgcagca ggcggtgagc aaactgcata tcggcgacgg
     5701 gctggataaa ggcgtcacca tcgggccgct gatcgatgaa aaagcggtag caaaagtgga
     5761 agagcatatt gccgatgcgc tggaaaaagg cgcgcgcgta gtttgcggtg gtaaagcaca
     5821 cgaacgcggc ggtaacttct tccagccgac cattctggtg gacgttccag ccaacgccaa
     5881 agtgtcgaaa gaagagacgt tcggccccct cgccccgctg ttccgtttta aagatgaagc
     5941 cgatgtgatc gcgcaggcca atgacaccga gtttggcctt gccgcctatt tctacgcccg
     6001 tgatttaagt cgcgtcttcc gcgtgggcga ggcgctggag tacggcatcg tcggcatcaa
     6061 taccgggatt atttccaatg aagtggcccc gttcggcggc attaaagcct cgggtctggg
     6121 tcgtgaaggt tcgaagtatg gcatcgaaga ttacttagaa atcaaatata tgtgcatcgg
     6181 tctttaactg gagaacgcga atgagcagca ataaagagtt aatgcagcgc cgcagtcagg
     6241 cagttcctcg tggcgttggg caaattcacc ccattttcgc tgatcgcgcg gaaaactgcc
     6301 gggtgtggga cgttgaaggc cgtgagtatc ttgatttcgc gggcggcatt gcggtgctca
     6361 ataccgggca cctgcatccg aaagtggttg cggcggtgga agcgcagttg aaaaaattgt
     6421 cgcacacctg cttccaggtg ctggcttacg agccgtatct ggagctgtgc gagattatga
     6481 atcagaaggt gccgggcgat tttgccaaga aaacgctgct ggtcaccacg ggttccgaag
     6541 cggtggaaaa cgcggtgaaa atcgcccgcg ctgccaccaa acgtagcggc accatcgctt
     6601 ttagcggcgc gtatcacggg cgcacgcact acacgctggc gctgaccggc aaggtgaatc
     6661 cgtactctgc gggcatgggc ctgatgccag ggcacgttta tcgcgcgctt tatccttgcc
     6721 cgctgcacgg catcagtgaa gatgacgcta tcgccagcat ccaccggatc ttcaaaaatg
     6781 acgccgcgcc ggaagatatc gccgccatcg tgattgagcc ggttcagggc gaaggcggtt
     6841 tctacgccgc gacgcctgcg tttatgcagc gtttacgcgc gctgtgtgac gagcacggga
     6901 tcatgctgat tgccgatgaa gtgcagagcg gcgcggggcg taccggcacg ctgtttgcga
     6961 tggagcaaat gggcgtggca ccggatctca ccacctttgc gaaatcgatc gcaggcggtt
     7021 tcccgctggc gggcgtcacc gggcgcgccg aagtgatgga tgccgtcgct ccaggcgggc
     7081 tgggcggcac ctatgcgggt aacccgattg cctgcgtggc tgcgctggaa gtgttgaagg
     7141 tgtttgagca ggaaaatctg ctgcaaaaag ccaacgatct ggggcagaag ttgaaagacg
     7201 gattgctggc gatcgccgaa aaacacaccg agatcggcga cgtacgcggg ctgggggcga
     7261 tgatcgccat cgagctgttt gaagacggcg atcacagtaa accgaacgcc aaactcaccg
     7321 ccgagatcgt ggcgcgcgcc cgcgataagg gcctgattct tctctcctgc ggcccgtatt
     7381 acaacgtgct gcgcatcctt gtaccgctca caattgaaga tgctcagatt cgtcagggtc
     7441 tggagatcat cggccagtgt tttgctgagg cgaagcagta gcaccgcccc catgccggag
     7501 gcgacgctgc gcgtcttgtc cggcctacga gagtgtaggt cggataaggc gtttacgccg
     7561 catccgacaa tcggcgcacc atgccggagg cgacgcttac gcgtcttgtc cggcctacgg
     7621 aaatgtaggt cggataaggc gtttgcgccg catccgacaa tcggcgcacc catgccggag
     7681 gcgacgcttt gcgtcttgtc cggcctacgg ggattctcgt cggataaggc gtttacgccg
     7741 catccgacaa tctgtacgtg aacaggaaga aatctatgtt ggccgggtaa ggcggagccg
     7801 ctctccggca aaaagaatca ataacaatta aacgcgtgac ccgacgcggg aaatgtcggg
     7861 gcgctctccc aagtgacaca ctttcgagag gattcaggat ggggcaatca tcgcaaccac
     7921 atgagttagg cggcgggctg aagtcacgcc acgtcaccat gttgtctatt gccggtgtta
     7981 tcggcgcaag tctgtttgtc ggttccagcg tcgccatcgc cgaagcgggc ccggcggtat
     8041 tactggccta tctgttcgcc ggattactgg tggttatgat tatgcggatg ttggcggaaa
     8101 tggcggttgc cacgcccgat accggttcgt tttccaccta tgccgataaa gccattggcc
     8161 gctgggcggg ctataccatc ggctggctgt actggtggtt ttgggtactg gttatcccac
     8221 tggaagccaa catcgccgcc atgatcctgc actcgtgggt tccaggcatt cccatctggt
     8281 tattttccct cgtcattacc ctcgccttaa ctggcagtaa tttattaagc gttaaaaact
     8341 acggcgaatt tgagttctgg ctggcgctgt gcaaagtcat cgctatcctg gcctttattt
     8401 tccttggtgc agtcgcaatt agcggttttt acccgtatgc cgacgtgagc gggatctcaa
     8461 gattgtggga tagcggcggc tttatgccca acggtttcgg tgcggtatta agtgcgatgt
     8521 tgatcaccat gttctcgttt atgggcgcag aaattgtcac cattgccgcc gcggaatccg
     8581 acacgccgga aaaacatatt gtccgcgcca ctaactcggt tatctggcgt atttctatct
     8641 tctatttgtg ctctattttt gtcgtcgtgg cattaatacc gtggaatatg ccggggctga
     8701 aagccgttgg ttcttatcgc tcggttctgg aattgctcaa tattccccat gcgaaattaa
     8761 tcatggactg cgtgatatta ctttccgtaa ccagttgcct gaactcggcg ctgtataccg
     8821 cttccaggat gctctactcc ttaagccgtc gcggtgatgc gcccgcggta atgggtaaaa
     8881 tcaaccgcag taaaaccccg tacgtggcgg tgttactctc caccggcgcg gcatttttaa
     8941 cggtggtggt gaactattac gcacctgcga aagtgtttaa attcctgatc gacagctccg
     9001 gcgctatcgc cctgctggtt tatttagtca tcgccgtttc acagttgcgg atgcgcaaaa
     9061 ttctgcgagc agaaggaagc gaaattcgcc tgcgcatgtg gctttacccg tggctcacct
     9121 ggctggtcat cggctttatt acctttgtgt tggtggtgat gctattccgc ccggcgcaac
     9181 agttagaagt gatctccact ggcttattag cgatagggat tatctgtacc gtgccaatta
     9241 tggctcgctg gaaaaagctg gtattgtggc aaaaaacacc cgttcataat acgcgctgat
     9301 aatgatcagg agtcacacca tgaccattac gtctctggat ggctatcgct ggctgaagaa
     9361 cgatattatt cgcggtaatt ttcaaccgga tgaaaaatta cgaatgaatt tgctgacatc
     9421 gcgttatgca cttggcgttg ggccgttacg ggaagctctt tcgcaactgg tggcggaacg
     9481 gctggtcacg gtggtgaatc aaaaagggta tcgggtggcg tctatgtcag agcaggagct
     9541 gctcgatatt ttcgacgccc gcgccaatat ggaagcgatg ttagtgagtc tggcgattgc
     9601 ccgcggtggc gatgagtggg aggcagacgt tctcgcaaaa gcgcatctgt tgagtaagct
     9661 tgaggcccgt gacgccagcg agaaaatgct tgatgagtgg gatctgcgtc atcaggcgtt
     9721 tcatacggca attgtggcgg gctgtggttc tcactatttg ctgcaaatgc gtgaacggtt
     9781 gtttgatctg gcggcgcgct atcgatttat ctggctgcga caaacagtgc tttcggtgga
     9841 aatgctggag gataaacacg atcagcacca gaccctgact gcggcggtac tggcgcgaga
     9901 taccgcgcgc gccagtgagt taatgtgcca gcatttactg acgccaattc ccattatcca
     9961 gcaggcgatg gctggcaatt aactactctt caggaatacg caacacttgc cccgggtaaa
    10021 ttttatccgg gctttttagc atcggtttat tcgcttcgaa gattttattg tacagattgg
    10081 cgttaccgta gacctgtttg gaaatggcac tcagagtgtc gccagactta acggtataaa
    10141 actggctggc agtggctggt gtcgccgttt tcacctgatc atcgacactg gcaataccgg
    10201 aaatattccc caccgcaaca aggattttct ccttcgcctc ctgactcagg ccgtcaccag
    10261 tgaccgtcgc tttgccgtcg gcaatttgaa tgttcacttt atcggcatca ggtataccgg
    10321 ttttgctcag atgctcctgc accttcttcg cctgatcgtc tttatcgtgc tgacctgtaa
    10381 ccgcgtccca gagtttttct ccggcatctt tcacaaaatt gaacagcccc atagctacct
    10441 cattcgttaa cggaaacact cagaaagtgt agcagaggaa cgcggcgcgt taacgccgcg
    10501 gggatgagca caggtttaat cgcgggtttg cacccagaac gcgtgaatca aaccggggat
    10561 atacccaagc agtgtcaaca gaatattaat aatgaacgcc caaccgaacc ctttaccgag
    10621 cagcacgccg agcggcggca gaatgatggt gatgacgatt ctccagaaac ccatatgtac
    10681 tccctataag aaaattactc attgtttaaa aagagatttt atctcttaag cgtagtaatt
    10741 ttagcggagg ctgccagttt cagcttacgc ttttactctc atttacgctg ctgatgctgg
    10801 tatatgttgc atttttagtt tagagtttac ttaatttagc taacacttaa atgactatga
    10861 ctgaactcgc acaattacag gccagtgccg aacaggcagc ggccttattg aaagcaatga
    10921 gccaccctaa acggttgctg attctgtgca tgcttagcgg ttcacccggc accagcgcgg
    10981 gagagctgac gcgcattacc ggactgagtg cctctgcgac atcacagcat ctcgcccgta
    11041 tgcgggacga agggcttatc gacagccaac gggatgccca acgcattcta tattccatta
    11101 aaaatgaggc ggtaaatgcc attatcgcca ccctgaaaaa tgtctattgt ccgtaaggag
    11161 tcactatggc tttgacaacc atttcgccgc atgatgcaca agaattaatc gcacgcggcg
    11221 caaagttaat cgatattcgt gacgctgatg aatatcttcg tgaacatatt cctgaagcag
    11281 atctggctcc attatccgtg ctggaacagt caggtctccc ggctaaatta cgtcgcgagc
    11341 aaattatatt ccactgccag gcaggtaaac gcaccagtaa taacgccgat aaattagcag
    11401 cgattgccgc ccccgcagaa atctttttac tcgaagatgg gattgatggc tggaaaaaag
    11461 caggattgcc agtagcggta aataaatctc aacccttgcc gttaatgcgc caggtgcaga
    11521 tcgctgcggg tggtttaata ttaatcggcg ttgtactggg ttataccgta aatagcggtt
    11581 tcttcttatt aagcggcttt gttggtgccg ggttactgtt tgcaggaatt agcggttttt
    11641 gtgggatggc aaggttgtta gataagatgc cgtggaacca atgagcttga gaagcgacgc
    11701 cggacgcgcc atagcagcga catccggcct cagtaattag atcaggaaat cgtcgagaga
    11761 tttaccttct gccagcgcct gagcgattgg cttcggtgta cggccctgac cggtccaggt
    11821 tttagtttca ccgttaacat cggtgaattt atatttcgcc ggacgcggct ggcgtttttt
    11881 accagcacgt ggtgcagcag cagagctatt acccaataac tcttccgggt taattccgtc
    11941 agctttcatc agctccagcc aggtgctaat tttttcctgg cgctctgcca gttcacgctg
    12001 ctgctgttct tcttcttcac gtctttcttt agtgacaacc ctgaattttt cgagcatttc
    12061 ttcaagaacg tcaatggaga attcgcgagc catcgcacgg agggtgcgaa tgttatttaa
    12121 actttgtaac attacggaca taataaagaa aaccttttaa cgccaaaaca aaaaagtatt
    12181 tcgtcgcgta gtgtaagcga tcacttatta tatttccaca aaatttaatc tatttacagt
    12241 cctgcgcgag attatttcag ctgttatttt ctgaaactct cagaataatt tcaagattat
    12301 atattagcca ttttgatgag taatttaggt tattggtgaa cagagtggcg ctctctgctc
    12361 gctaattccg caagcctgac aaaaccgaag tgaataaaaa tgcaatataa cagaataaaa
    12421 gaaaacacca ctcactggtg ctttctgctg ttaatgtaca ttttctggca taaaaaatgc
    12481 tgtaaatgac ccggtcacga catacaccga attctctacg cttatagcgt tacctcaccc
    12541 ccaaactttc attagtgaaa ttaaaggaaa ggtcatatct aattgattta attgaaaata
    12601 aatcatatcg gcatgtaaaa atccacaatg tacaaaaaac gagccgttac ggaatatttt
    12661 atctacaaaa actgactaaa taaaaatttt tcactaattg attagtcata gtcagcgata
    12721 tacgctatgc gaaaatgcag atggcaatga gatccactgc tttcatctcc attaacatcc
    12781 cattacgctt ttattaagga gcattagcat gttctcaccg cagtcacgct tgcgtcatgc
    12841 agttgcagat acgttcgcga tggttgttta ctgttctgtc gtgaacatgt gtattgaagt
    12901 tttcctctcc ggaatgagct tcgaacagtc tttttattcc agattggtag cgattccggt
    12961 gaacatctta attgcatggc catacggtat gtaccgcgat ctgtttatgc gcgcggcacg
    13021 caaaattagc ccgtcgggct ggataaaaaa tctggcggat atcctggctt atgtgacgtt
    13081 ccagtcaccg gtgtatgtgg cgatcttgtt agtggtgggc gcagactggc atcagattat
    13141 ggcggcggtc agttcaaaca tcgttgtttc gatgttgatg ggggcggttt atggctactt
    13201 cctcgattat tgccgccgac tgtttaaagt cagccattac cagcaggtaa aagcctgact
    13261 cttcttttcg cgactggcat cgccagtcgc gtctttaact ggcttcgcca aataaaccat
    13321 tcaaataacg ttcaagcgca acacgcgaac taaagccatg cgggataccg taatgctcgt
    13381 gtcgctcgcc tgctaaatag agcggaaatt gttgataatg atcgcaagtt ttaatatcgg
    13441 aagcgggttc agccagtgtc gaactacgtt cttttaatgt tggatgaatt accagtgcgg
    13501 tacgtcccat tcgcgcttcg cgattaacat agacataatt ttccccacgg cggtaaccat
    13561 acgctttctg ggttaccaca tcgacagtaa aaccgacttt ttcaagtacg cgcgccacct
    13621 cgtctggtcg taaatacata tttgatcctc gttattatca accgcgggcc taccttacct
    13681 gattgcgcat tcacaacgct ttcagaaaag tccataaagc cgcgatgaac ctcagttaag
    13741 aaaattatgg tctacactga aaattacatc gaattctaat gggggatcat atgtttaacc
    13801 gaccgaaccg caacgacgtt gatgatggcg tgcaggatat ccagaatgat gtcaatcaat
    13861 tagctgacag cctcgaatct gtattgaagt cctggggcag cgacgccaaa ggggaagctg
    13921 aagccgcacg cagtaaagct caggcattgc tgaaagaaac cagggcgcga atgcatggtc
    13981 gtactcgcgt ccagcaagcc gcacgcgatg ccgttggctg cgcggattct tttgttcgtg
    14041 aaagaccctg gtgtagtgtg ggaactgctg ctgcggtagg tatttttatc ggtgcgttgt
    14101 tgagcatgcg taaatcgtag tgagacgatg acaagcagta gtctgacttc tgaaaagtac
    14161 tcagtgcgtc aacgccttgt ccgcctacag gcctgaaaaa catagcgcat caggggattt
    14221 tgccctggtc accagtctta taggaccccg aagcctggct tcggggttat taatttaaag
    14281 cactgcgcaa ttgctgtgct acctgtcgcg ccatactttc gctaacgata ttcgtaaacg
    14341 agagcaataa tcctccgtcc tcgcttgagc ggatacgcca gtcgctaagt gcctgtacgg
    14401 caagaccagc agcattggct ttatgcacga atgtcacgtc attacctgct agtttgatca
    14461 ccatctggat accgcctttt tgcggcacaa cctgaaatcc ctgctgcgta agtgcctgct
    14521 caatccactg ccgtcgctgg gcataatgct ggcgcatttt cttcagatgc cgccagaaat
    14581 gcccctcacg gatgaaatct gccagtgtgt tctgccacag tacgggtaca gcacatggtt
    14641 ccagtgacgc ctgctgccgg aattgcgaaa tctgctccac cggcaccacc agccacgcac
    14701 agcgcaatgc aggaaatagc gctttgctga atgttccggc ataaatcacc cgctgcggcg
    14761 cgtcgagact ttttagtgcc ggtaacggct taccgtgata gcgaaactca ctgtcgtaat
    14821 catcttcaat aatccatgcc tgactacgct ctgcccattc cagtatctga tacctacgcg
    14881 ctaaagagag tgccacaccc agcgggcttt ggtgtgccgg agtaatcagg gcaaaacgcg
    14941 catcaggata attttgtatt ccgcttgtga catccagtcc gttgtcatca accggcacag
    15001 gtaaaatttc cacaccgtgg cgagtgacaa tcgggcgaat cagcggaaag ccgggatctt
    15061 ccatccacat cccgtctccc ggttgcgcca gagcgtgcag aattaaagcc attgaggccg
    15121 cataaccgtg agtgataaag acctgctctg gctgacaatc aatcccacgt gaaacgcgta
    15181 aataatcaac aatagcctcg cgcaaggccg cctcgccgca gacatcgcct aatgccaaat
    15241 caaaacgcgt ctgggtacgc agacgacggc ccatcacccg cgcccacaat tcacgcggaa
    15301 aaagatcaag tgcaggtagc cccatctgaa aaggcagcgg ctgctgattt tcaccagcaa
    15361 aagccacgac atccacagca gactgttgcg gatgtatctg ctcactgaca aaggtacccg
    15421 cctgtccccg cctgaccagc catccctgcg ccaccagctc agcataagca ttttcgacag
    15481 tggatcgaga gacacccagc tcctgcgacc aggtacggct cgagggtagc cttgcgccag
    15541 gtttcagttc tccttgctcg atagccgttt ttaactgacg ggcgatatcc tgataacgtg
    15601 gcatatggtc tgctttttta ggtaatttat ggctctcttt agcagtccat tatagtgcta
    15661 cattgcgggc gttaataaga ggagaaactg tatgactaca ttacgtcagc cttactacga
    15721 acttagcccg gcagtgtata acgcactggt gcaggccaaa acggcactgg aaaatagcac
    15781 gctggatacc acgttgatgg agttaattta tttgcgcgtc tcgcaaatca acggctgcgc
    15841 attttgtctg gagatgcaca gcaaagcatt gcgcaaatcc ggtgtgccac aacacaaact
    15901 ggacgccctg gcaggctggc gcgtaagcca tcattttgat gaacgcgagc gagcggcgct
    15961 ggcatgggcg gaatcagtga ccgatattgc caaaacccat gcggaagacg aggtttatca
    16021 gcctttgctt gagcatttca gcgcagcaga aatcagcgac ttaacgtttg ccatcgggct
    16081 gatgaattgt tttaaccgtc tggccgtttc catgcggatg taatttgctg ttgccgggta
    16141 tgcgcccggc aaaaaaacac aaaatcccct accccgttga gccattcacc ggtggcgtaa
    16201 tcaatctaaa attcccatat cttgtgtggt tgaatctttt ttcaactaca tctagtatct
    16261 ctgtatcaac agagagacaa cccgacgcgt atcatcgcgc cgtatcttca ttttaaacgg
    16321 aaatacgaat catgcgcatt actatttaca ctcgtaacga ttgcgttcag tgccacgcca
    16381 ccaaacgggc gatggaaaac cggggctttg attttgaaat gattaatgtc gatcgcgttc
    16441 ctgaagcggc agaagcgttg cgtgctcagg gctttcgtca gttgccggta gtgattgctg
    16501 gcgatcttag ctggtctggt ttccgtccgg acatgattaa ccgtctgcat ccagcgccac
    16561 acgcggccag tgcatgagcc agctcgtcta cttctccagc agctccgaaa acacgcagcg
    16621 ttttatcgaa cgtttaggtc tgcccgcggt gcgcatcccg ctcaatgagc gggaacggat
    16681 tcaggtagac gagccttaca tcctgatcgt gccctcttac ggcggcggcg gtacggctgg
    16741 cgcggtgcca cgacaggtaa ttcgcttttt aaacgacgag cacaaccggg cgttgcttcg
    16801 cggcgttatt gcttcgggta atcgcaactt tggtgaggcg tatggccgcg ctggagatgt
    16861 gattgcccgg aaatgcggcg tgccgtggct gtaccgcttt gaactcatgg gtacgcaaag
    16921 cgatatcgaa aacgttcgta aaggagtaac cgaattttgg caacgacaac cgcagaatgc
    16981 ctgacgcagg aaacgatgga ttaccacgcg ctgaatgcga tgcttaacct ctacgatagc
    17041 gcaggtcgca ttcagttcga taaagaccgc caggccgttg acgcctttat tgcgacgcat
    17101 gtgcgtccga acagtgtgac cttcagtagc cagcagcagc gcctgaactg gctggtcaac
    17161 gaaggttact atgatgaaag cgttcttaat cgctactctc gcgactttgt cattacgctg
    17221 tttgaccacg cacacaccag cggttttcgt ttccagacat tcctcggggc atggaagttt
    17281 tacaccagct atacgttgaa gacattcgac ggtaaacgtt atctggaaga ttttgccgat
    17341 cgagtcacga tggtggcgct gacgctggca caaggcgatg agacgctggc gttgcaactg
    17401 acagatgaaa tgctgtcagg acgctttcag ccagccacgc caacattcct caactgcggt
    17461 aagcagcagc gcggcgaact ggtttcctgt tttttgctgc gtattgaaga caatatggag
    17521 tcgattggtc gggcggtaaa ttccgcactg caactgtcga aacgcggcgg cggcgtagca
    17581 tttttgctgt cgaatctgcg ggaagcgggc gcgccaatta aacgtattga aaatcaatct
    17641 tctggcgtaa ttccggtgat gaaaatgctg gaagacgcat tttcctatgc caaccaactc
    17701 ggcgcgcgtc agggggctgg ggcagtctat ttacatgctc atcatcccga tattctgcgt
    17761 tttctcgaca cgaaacggga aaatgccgac gaaaaaatcc gcattaaaac actgtcgctt
    17821 ggcgtggtga tcccggatat cactttccat ctggcaaaag agaatgcgca gatggcgctg
    17881 ttttcgcctt atgacgtaga gcgagtttat ggcaagccgt ttgccgatgt ggccatcagt
    17941 caacactatg acgaactggt tgtcgatgaa cgcattcgaa aaaaatacct caacgcccgt
    18001 gatttcttcc agcgactggc agaaatccag tttgagtccg gctatccgta catcatgtat
    18061 gaagacacgg taaaccgtgc taaccctatc gccgggcgca taaatatgag taatctctgc
    18121 tcagagattt tgcaggttaa cagcgcctca gagtataacg agaatctcga ctatgcccgc
    18181 acaggccatg atatctcctg caatttaggt tcgttgaata ttgcccacac catggattcc
    18241 cccgactttg cccgcacggt agagactgcc gtgcgcggtt taacggcagt atcagatatg
    18301 agtcatatcc gcagcgtgcc gtccatcgaa gccggaaatg ccgcctcgca cgccatcgga
    18361 ctggggcaga tgaatttaca cggctatctg gcgcgagaag gcatcgctta tggttcgccg
    18421 gaagcactgg atttcaccaa tctctatttc tatgccatca cctggcacgc actgcgtacc
    18481 tcgatgttgc tggcacgcga acgcggtgaa accttcgccg ggttcaaaca gtcacgctat
    18541 gccagcggtg aatattttag ccaatatctg caaggtaact ggcagccgaa aaccgcgaaa
    18601 gttggcgaac tgtttgcccg cagcggtatt acgttaccta cccgtgagat gtgggcgcag
    18661 ctgcgcgatg acgtgatgcg ctacggcatt tacaaccaga atctccaggc ggtgccgcca
    18721 accggttcta tctcttatat caaccatgct acgtcgagta ttcatccgat tgtggcgaaa
    18781 gtagagatac gcaaagaggg caaaacagga cgcgtttact accctgcccc gtttatgact
    18841 aacgagaatc tggcgctgta tcaggacgct tacgaaattg gcgcagaaaa gatcatcgac
    18901 acctacgcgg aagcgactcg ccatgtcgat caggggctgt cgctgacgct gtttttcccc
    18961 gataccgcca ccactcgcga tatcaacaaa gcgcagattt acgcctggcg caaaggtatc
    19021 aaaacgctct attacatccg cctgcgtcag atggcgctgg aaggcactga aattgaaggc
    19081 tgcgtctcct gtgcacttta aggaatatct atgaaactct cacgtatcag cgccatcaac
    19141 tggaacaaga tatctgacga taaagatctg gaggtgtgga atcgcctgac cagcaatttc
    19201 tggctaccag aaaaggtgcc actgtcgaac gatatacctg catggcagac attaagtgtc
    19261 gtagaacaac aactgacaat gcgcgttttt actggcctga cgctgctcga cacgctgcaa
    19321 aatgttatcg gcgcgccttc gctgatgccc gatgcgctca cgccccatga agaggcggta
    19381 ttatcgaata tcagctttat ggaagcggtt catgcccgtt cttacagttc gattttctcg
    19441 acgttgtgcc agaccaaaga tgtcgatgcc gcctacgcct ggagtgaaga aaacgcaccg
    19501 ttgcagcgaa aagctcagat tattcagcaa cattatcgcg gtgatgatcc gctgaaaaag
    19561 aaaatcgcca gtgtgtttct tgaatctttt ttgttctatt ccggtttctg gctgccgatg
    19621 tatttttcca gccgcggaaa gctgaccaat accgcggacc tgatccgtct gattatccgc
    19681 gatgaagcag tccacggtta ctacataggc tataaatatc agaaaagact ggaaaagatc
    19741 tccgaggcac aacgtgaaga gttgaagagt ttcgccttcg atttgttgct agaactctat
    19801 gacaacgagt tgcaatatac cgatgagctg tacgccgaaa ccccgtgggc tgacgatgtg
    19861 aaagcgtttc tctgttacaa cgccaataag gcgttgatga atctgggcta cgaaccttta
    19921 tttcccgcag aaatggcgga agtgaatccg gcaatcctcg ccgcgctttc gccgaatgcc
    19981 gatgaaaatc acgatttctt ttccggttca ggctcctctt atgtgatggg gaaagcggtt
    20041 gaaacagaag atgaagactg gaatttctga gggtgttatt ttcaaaaata tcactacccg
    20101 cgacagggaa ataattcccg ccaaatagct ttttatcacg caaatatttt gtggtgatct
    20161 acactgatac tctgttgcat tattcgcctg aaaccacaat attcaggcgt tttttcgcta
    20221 tctttgacaa aaaatatcaa cttttctcga tttgctctca gcccttatat cacgggaaat
    20281 tccggcgatt tgctcacatc aatattcatg ccacatttgc cctcaggggt tgcctcagat
    20341 tctcagtatg ttagggtaga aaaaagtgac tatttccatt gggtaatgta tcgacataaa
    20401 caaataacag gaatctttct attgcatggc aattaaatta gaaattaaaa atctttataa
    20461 aatatttggt gagcatccac agcgagcttt caaatatatc gaacaaggac tttcaaaaga
    20521 acaaattctg gaaaaaactg ggctatcgct tggcgtaaaa gacgccagtc tggccattga
    20581 agaaggcgag atatttgtca tcatgggatt atccggttcg ggtaaatcca caatggtacg
    20641 ccttctcaat cgcctgattg aacccacccg cgggcaagta ctgattgatg gtgtggatat
    20701 tgccaaaata tccgacgccg aactccgtga ggtgcgcaga aaaaagattg cgatggtctt
    20761 ccagtccttt gccttaatgc cgcatatgac cgtgctggac aatactgcgt ttggtatgga
    20821 attggccgga attaatgccg aagaacgccg ggaaaaagcc attgatgcac tgcgtcaggt
    20881 cgggctggaa aattatgccc acagctaccc ggatgaactc tctggcggga tgcgtcaacg
    20941 tgtgggatta gcccgcgcgt tagcgattaa tccggatatt ttattaatgg atgaagcctt
    21001 ctcggcgctc gatccattaa ttcgcaccga gatgcaggat gagctggtaa aattacaggc
    21061 gaaacatcag cgcaccattg tctttatttc ccacgatctc gatgaagcca tgcgtattgg
    21121 cgaccgaatt gccattatgc aaaatggtga agtggtacag gtcggcacac cggatgaaat
    21181 tctcaataat ccggcgaatg attatgtccg taccttcttc cgtggcgttg atattagtca
    21241 ggtattcagt gcgaaagata ttgcccgccg gacaccgaat ggcttaattc gtaaaacccc
    21301 tggcttcggc ccacgttcgg cactgaaatt attgcaggat gaagatcgtg aatatggcta
    21361 cgttatcgaa cgcggtaata agtttgtcgg cgcagtctcc atcgattcgc ttaaaaccgc
    21421 gttaacgcag cagcaaggtc ttgatgcggc gctgattgat gcgccgttag cagtcgatgc
    21481 acaaacgcct cttagcgagt tgctctctca tgtcggacag gcaccctgtg cggtgcccgt
    21541 ggtcgacgag gaccaacagt atgtcggcat catttcgaaa ggaatgctgc tgcgcgcttt
    21601 agatcgtgag ggggtaaata atggctgatc aaaataatcc gtgggatacc acgccagcgg
    21661 cggacagtgc cgcgcaatcc gcagacgcct ggggtacacc ggcgactgca ccgactgacg
    21721 gcggtggtgc tgactggctg accagtacgc ctgcgccaaa cgtcgagcat tttaatattc
    21781 tcgatccgtt ccataaaacg ctgatcccgc tcgacagttg ggtcactgaa gggatcgact
    21841 gggtcgttac ccatttccgt cccgtcttcc agggcgtgcg cgttccggtt gattatatcc
    21901 tcaacggttt ccagcaattg ctgctgggta tgcccgcgcc ggtggcgatt atcgttttcg
    21961 ctctcatcgc ctggcagatt tccggggtcg gaatgggcgt ggcgacgctg gtttcgctga
    22021 ttgccatcgg cgcaatcggt gcctggtcgc aggcaatggt gactctggcg ctggtgttaa
    22081 ccgccctgct gttctgtatc gtcatcggtt tgccgttggg gatctggctg gcgagaagtc
    22141 cgcgagcggc gaaaattatt cgtccactgc ttgatgccat gcagaccacg ccagcgtttg
    22201 tttatctggt gccaatcgtc atgctatttg gtatcggtaa cgtgccgggc gtggtggtga
    22261 cgatcatctt tgctctgccg ccgattatcc gtctgaccat tctggggatt aaccaggttc
    22321 cggcggatct gattgaagcc tcgcgctcat tcggtgccag cccgcgccag atgctgttca
    22381 aagttcagtt accgctggcg atgccgacca ttatggcggg cgttaaccag acgctgatgc
    22441 tggccctttc tatggtggtc atcgcctcga tgattgccgt cggcgggctg ggtcagatgg
    22501 tacttcgcgg tatcggtcgt ctggatatgg ggcttgccac cgttggcggc gtcgggattg
    22561 tgatcctcgc cattatcctc gatcgtctga cgcaggccgt tgggcgcgac tcacgcagtc
    22621 gcggcaaccg tcgctggtac accactggcc ctgtcggtct gctgacccgc ccattcatta
    22681 agtaactctg cacttgcccg gtgacaccgg gcatatcacc ctgccaaaaa aaaggaataa
    22741 caatgcgaca tagcgtactt tttgcgacag cgtttgccac gcttatctct acacaaactt
    22801 ttgctgccga tctgccgggc aaaggcatta ctgttaatcc agttcaaagc accatcaccg
    22861 aagaaacctt ccagacactg ctggtcagtc gtgcgctgga gaaattaggt tataccgtca
    22921 ataaacccag cgaagtggat tacaacgttg gctacacctc gcttgcttcc ggcgatgcaa
    22981 ccttcaccgc cgtgaactgg acaccactgc atgacaacat gtacgaagct gccggtggcg
    23041 ataagaaatt ttatcgtgaa ggggtatttg ttaacggcgc ggcacagggt tacctgatcg
    23101 ataagaaaac cgccgaccag tacaaaatca ccaacatcgc acaactaaaa gatccgaaga
    23161 tcgccaaact gttcgatacc aacggcgacg gtaaagcgga tttgactggc tgtaaccctg
    23221 gctggggctg cgaaggtgcg atcaaccacc agcttgccgc gtatgaactg accaataccg
    23281 tgacgcataa tcaggggaac tacgcggcga tgatggctga caccatcagt cgttacaaag
    23341 agggcaaacc ggtgttttac tacacctgga cgccgtactg ggtgagtaat gagctgaagc
    23401 cagggaaaga tgtggtctgg ttgcaggtgc cgttctctgc actgccgggc gataaaaatg
    23461 ccgataccaa actgccgaat ggcgcgaatt acggcttccc ggtcagcacc atgcatatcg
    23521 ttgccaataa agcctgggcc gagaaaaacc cggcagcagc gaaactgttt gccattatgc
    23581 agttgccagt ggcagatatt aacgcccaga acgccattat gcatgacggc aaagcctcag
    23641 aaggcgatat tcagggccac gttgatggct ggatcaaagc ccaccagcaa cagttcgatg
    23701 gctgggtgaa tgaggcgttg gcagcgcaga agtaattttt attcgggcgg ataaggcgtt
    23761 tacgccgcat ccgccgttct gtacacaatg cctgatgcga cgctggcgcg tcttatcatg
    23821 cctacaaacc acaccgcaca tttcaacaat ctatctttca tcccatattc atcaacatcc
    23881 gctattattg atttccagct taatcatcac ctgatgaaca aaaataatga ccaaacctaa
    23941 tcatgagctt agcccggcgc taatcgtgct gatgtctatc gccaccggtc tggcggtagc
    24001 cagtaactat tacgcccagc cattgctcga caccatcgcg cgtaactttt ccctttccgc
    24061 cagttcggca ggctttattg ttaccgccgc gcagttgggc tatgccgcag gtctactgtt
    24121 tcttgttccc ctcggtgata tgtttgaacg ccgccgcctg attgtctcga tgaccttact
    24181 ggcggcatgc ggcatgttga ttaccgccag cagtcagtcg ctggcgatga tgatcctcgg
    24241 tacggcatta accggtttat tctcagtcgt ggcacaaatt ctggttccac tggcagcgac
    24301 gctggcttca ccggataaac gcggcaaagt ggttggcacc attatgagcg gcctgctgtt
    24361 ggggatattg ctggcacgaa ccgttgccgg gttactggca aacctcggcg gctggcgcac
    24421 cgtcttttgg gttgcctcgg tgttaatggc tctgatggcg ctagcattat ggcgtggtct
    24481 gccacaaatg aaatcagaaa cccacctcaa ttacccacag ttgttgggtt ccgttttcag
    24541 tatgtttatc agcaataaga tcctgcgcac ccgcgcgttg ctgggctgcc tgacctttgc
    24601 caacttcagc attctctgga cctcaatggc ctttttgctt gccgcaccgc cttttaatta
    24661 caacgatggc gtcattggtc tgtttggact tgcgggggct gccggagcgt tgggcgctcg
    24721 tccggcgggc ggttttgccg acaagggcaa atcgcaccac accacaactt tcggtctgct
    24781 gctgctgtta ctttcatggc tggcaatctg gtttggtcac acttccgtac tggcgttgat
    24841 tatcggcatt ctggtgctgg acctcaccgt acagggcgtg catatcacta accagacggt
    24901 aatttatcga atacatcctg atgcgcgtaa tcgcctgacc gcaggttaca tgaccagcta
    24961 ctttattggc ggtgccgccg gttcgctaat ttcagcctca gcctggcaac atggcggttg
    25021 ggctggcgtt tgtctggctg gcgcgacgat tgccctggtt aacttactgg tctggtggcg
    25081 aggttttcat cgtcaggaag ccgcaaatta agcaatcagc aaccgttttc gtggctttac
    25141 acttataagg gtgttaagaa gcccatcagt ctgataaggt taagatattc attcagtcta
    25201 tttataatat taacaatcgt taagcgtaca ctctatggaa agccctacta cacagcctgc
    25261 tcctggttcg gcgaccttca tggaaggatg caaagacagt ttaccgattg ttattagtta
    25321 tattccggtg gcctttgcgt tcggtctgaa tgcgacccgt ctgggattct ctcctctcga
    25381 aagcgttttt ttctcctgca tcatttatgc aggcgcgagc cagttcgtca ttaccgcgat
    25441 gctggcagcc gggagtagtt tgtgggttgc tgcactgacc gtcatggcaa tggatgttcg
    25501 ccatgtgttg tatggcccgt cactgcgtag ccgtattatt cagcgtctgc aaaaatcgaa
    25561 aaccgccctg tgggcgtttg gcctgacgga tgaggttttt gccgccgcaa ccgcaaaact
    25621 ggtacgcaat aatcgccgct ggagcgagaa ctggatgatc ggcattgcct tcagttcatg
    25681 gtcatcgtgg gtatttggta cggtaatagg ggcattctcc ggcagcggct tgctgcaagg
    25741 ttatcccgcc gttgaagcag cattaggttt tatgcttccg gcactcttta tgagtttcct
    25801 gctcgcctct ttccagcgca aacaatctct ttgcgttacc gcagcgttag ttggtgccct
    25861 tgcaggcgta acgctatttt ctattcccgt cgccattctg gcaggcattg tctgtggctg
    25921 cctcactgcg ttaatccagg cattctggca aggagcgccc gatgagctat gaggttctgc
    25981 tgcttgggtt actggttggc gcggcgaatt attgcttccg ctatttgccg ctgcgcctgc
    26041 gtgtgggtaa tgcccgccca accaaacgtg gcgcggtagg tattttgctc gacaccattg
    26101 gcatcgcctc gatatgcgct ctgctggttg tctctaccgc accagaagtg atgcacgata
    26161 cacgccgttt cgtgcccacg ctggtcggct tcgcggtact gggtgccagt ttctataaaa
    26221 cacgcagcat tatcatccca acactgctta gtgcgctggc ctatgggctc gcctggaaag
    26281 tgatggcgat tatataaccc acaagaatca tttttctaaa acaatacatt tactttattt
    26341 gtcactgtcg ttactatatc ggctgaaatt aatgaggtca tacccaaatg gatagttcgt
    26401 ttacgcccat tgaacaaatg ctaaaatttc gcgccagccg ccacgaagat tttccttatc
    26461 aggagatcct tctgactcgt ctttgcatgc acatgcaaag caagctgctg gagaaccgca
    26521 ataaaatgct gaaggctcag gggattaacg agacgttgtt tatggcgttg attacgctgg
    26581 agtctcagga aaaccacagt attcagcctt ctgaattaag ttgtgctctt ggatcatccc
    26641 gtaccaacgc gacgcgtatt gccgatgaac tggaaaaacg cggttggatc gaacgtcgtg
    26701 aaagcgataa cgatcgccgc tgcctgcatc tgcaattaac ggaaaaaggt cacgagtttt
    26761 tgcgcgaggt tttaccaccg cagcataact gcctgcatca actctggtcc gcgctcagca
    26821 caacagaaaa agatcagctc gagcaaatca cccgcaaatt gctctcccgt ctcgaccaga
    26881 tggaacaaga cggtgtggtt ctcgaagcga tgagctaacg cgtcatctcg ctcaaaaatc
    26941 cagatttata aaagaaaaaa tgaccggcca gcatcgcaac atgctggcct ttttggcaag
    27001 caggtcggct cagccgatga gttaagaaga tcgtggagaa caatatgagc gcaaatgcgg
    27061 agactcaaac cccgcagcaa ccggtaaaga agagcggcaa acgtaagcgt ctgctcctcc
    27121 ttctcacctt gctctttata attattgccg tagcgatagg gatttattgg tttttagtac
    27181 tgcgtcattt cgaagaaacc gatgacgcat acgtggcagg gaatcaaatt caaattatgt
    27241 ctcaggtgtc tggcagcgtg acgaaagtct gggccgataa caccgatttt gtaaaagaag
    27301 gcgacgtgct ggtcactctc gacccgacag atgctcgcca gacgtttgaa aaagccaaaa
    27361 ctgcactggc ttccagcgtt cgccaaaccc accagctgat gattaacagc aaacagttgc
    27421 aggcgaatat tgaggtgcaa aaaatcgccc tcgcgaaagc acaaagcgac tacaaccgcc
    27481 gtgtgccgct gggcaatgcc aacctgattg gtcgcgaaga gctgcaacac gcccgcgacg
    27541 ccgttaccag tgcccaggcg caactggacg tcgcgattca acaatacaat gccaatcagg
    27601 cgatgattct ggggactaaa ctggaagatc agcctgccgt gcaacaggct gccaccgaag
    27661 tacgtaacgc ctggctggcg ctggagcgta ctcgtattgt cagtccgatg accggttatg
    27721 tctcccgccg cgcggtacag cctggggcgc aaattagccc aacgacgccg ctgatggcgg
    27781 tcgttccagc caccaatatg tgggtggatg ccaactttaa agagacgcag attgccaata
    27841 tgcgtatcgg tcagccggtc actatcacca ccgatattta cggcgatgat gtgaaataca
    27901 ccggtaaagt ggttggtctg gatatgggca caggtagcgc gttctcactg cttccggcgc
    27961 aaaatgcgac cggtaactgg atcaaagtcg ttcagcgtct gcctgttcgt atcgaactgg
    28021 accaaaaaca gctggagcaa tacccgctgc gtatcggttt atccacgctg gtgagcgtca
    28081 ataccactaa ccgtgacggt caggtactgg caaataaagt acgttccaca ccggtagcgg
    28141 taagcaccgc gcgtgaaatc agcctggcac ctgtcaataa actgatcgac gatatcgtaa
    28201 aagctaacgc tggctaatcc agaggtgcgt gtgatgcaac agcaaaaacc gctggaaggc
    28261 gcgcaactgg tcattatgac gattgcgctg tcactggcga cattcatgca ggtgctggac
    28321 tccaccattg ctaacgtggc gatccccact atcgccggga atctgggctc atcgctcagc
    28381 cagggaacgt gggtaatcac ttctttcggg gtggcgaatg ccatctcgat cccgcttacc
    28441 ggctggctgg caaagcgcgt cggggaagtg aaactgttcc tctggtccac catcgccttt
    28501 gctattgcgt cgtgggcgtg tggtgtctcc agcagcctaa atatgctgat cttcttccgc
    28561 gtgattcagg ggattgtcgc cgggccgttg atcccgcttt cgcaaagtct attgctgaat
    28621 aactacccgc cagccaaacg ctcaatcgcg ctggcgttgt ggtcgatgac ggtgattgtc
    28681 gcgccaattt gcggcccgat cctcggcggc tatatcagtg ataattatca ctggggctgg
    28741 atattcttca tcaacgtgcc gattggcgtg gcggtggtgt tgatgacatt gcagacgctg
    28801 cgcggacgcg aaacccgcac cgaacggcgg cggattgatg ccgtggggct ggcactgctg
    28861 gttattggta tcggcagcct gcagattatg ctcgaccgcg gtaaagagct ggactggttt
    28921 tcatcacagg aaattatcat ccttaccgtg gtggcggtgg tggctatctg cttcctgatt
    28981 gtatgggagc tgaccgacga taacccgata gtcgatctgt cgttgtttaa gtcgcgcaac
    29041 ttcaccatcg gctgcttgtg tatcagcctc gcgtatatgc tctacttcgg cgctattgtt
    29101 ctgctgccgc agttgttgca ggaggtctac ggttacacgg caacgtgggc aggtctggcc
    29161 tctgcgccgg tagggattat tccggtgatc ctgtcgccga ttatcggccg cttcgcgcat
    29221 aaactggata tgcgccgact ggtaaccttc agctttatta tgtatgccgt ctgcttctac
    29281 tggcgtgcct atacctttga accgggtatg gattttggcg catcggcctg gccgcagttt
    29341 atccagggct tcgctgtggc ctgcttcttt atgccgctga ccaccattac gctgtctggt
    29401 ttgccaccgg aacgactggc ggcggcatcg agtctctcca actttacgcg tacgctggcg
    29461 gggtctatcg gcacgtcgat aaccacaacc atgtggacca accgcgagtc gatgcaccat
    29521 gcgcagttaa ccgagtcggt aaacccgttc aacccgaatg cccaggcgat gtacagtcaa
    29581 ctggaagggc ttgggatgac gcaacagcag gcctccggct ggattgccca gcagatcact
    29641 aaccaggggc tgattatttc cgccaatgag atcttctgga tgtcagccgg gatattcctc
    29701 gtcctgctgg ggctggtgtg gtttgctaaa ccgccctttg gcgcaggtgg cggcggaggc
    29761 ggtgcgcact aagtacaact aagccagttc atttgaactg gcttttttca attaattgtg
    29821 aagatagttt actgactaaa tgtgcagttc ctgcaacttc tctttcggca gcgccagttc
    29881 ttcgttgctg ttgatgcgca cgtcacgttc cagaatgcta cgcgcaatat cctgcgcttc
    29941 ctgcaacgag tgcatctggt aagtgccaca ctggtagacg ttcagctccg gaatctgatt
    30001 ctgatcctgc actttcagca cgtcttccat tgccgctttc caggcatcag caacacgctg
    30061 ctcatctggc gtaccaatca gactcatata aaaaccggtg cggcagccca ttggcgagat
    30121 atcgataatc tctacaccat taccgttaag atggttacgc ataaaaccag caaacaggtg
    30181 ctccagggta tggatccctc tttctggcat cacttctttg ttcggcacgc agaagcgcag
    30241 atcgaacacg gtgattgcgt cgccatgcgg ggtgttcatt gttttcgcca cccgaactgc
    30301 aggcgcttcc atccgggtat gatcgactgt gaagctatct aacaacggca tttagccacc
    30361 tccggtaatt tttttaaaaa ttttctgaac tctttcttcc caggcgagtc tgagtatatg
    30421 aaagacgcgc atttgttatc atcatccctg aattcagaga tgaaattttg gccactcacg
    30481 agtggccttt ttcttttctg tcaggcgtgt ttttccagcc acaccgcaaa cggttcggta
    30541 tctgcggctt ccatttcctg ctgacggcgt tcagacgcct cgcgctcggc tacaaaatcc
    30601 tcttcgcgca gaatttccag cggctcttca cgcagcagat tacggtaggc ttctgcaaat
    30661 gctttgcctg ttccgccaat accagtatca atcatagacc ttaagatacg ggcagagaaa
    30721 gtcagatcgg gattatcgaa gcaggcgacc agttcatcac acactttctg atacgcttcg
    30781 ccgccgttaa tgctatccag cgtttgcgcg acgcgtttca gatcgcggaa cagatcttta
    30841 cccacctgcg gtaacgggaa ctgcgcggtt tcgcagccga tacccagcgt cagacccggt
    30901 ttgcgacctt cgaggatcac ccggttccag ttaacgcgtg tacaggcaag ttcgctactg
    30961 ctcatttccg gtgcatcagc cagcgcacac cagaccataa acaggtcgag gaatcgcacc
    31021 tgctgttcat ctacaccaat cggcgagaac gggttgatgt ccagcgaacg cacttcaata
    31081 tattcaatgc cgccacgtaa cagcgcatca gaaggcgact cgccgctgcg ggtaacgcgt
    31141 tttggacgaa tcggcgcgta cagttcgttt tcaatctgca gcacattgct attgatttgc
    31201 agccgcttac catctttctc aataccaatc ttcgcgtact cttccgatgg cgttttgatt
    31261 gcctgtttca ggcccgctac gtactcgtaa agatcgttga aggtaatacc aagattgctt
    31321 tgcgatttat tggtatagcc aagatcgctc aaacgaagag aggtcgcata cggcaggtaa
    31381 tacataccgc actcggtttt ctcaaacggc agcgacgttg gtttaccttg caggaatgaa
    31441 gagcatattg ccggagacgc gccaaacaga taaggaatga cccaaccgaa gcgatagtag
    31501 ttgcgaataa cgcggaaata gcccgcagag attttctctt tagcatcagc gcccgaaata
    31561 tcgccacact tcgcttgcca gaatgccata ggcaaagaga agttgtaatg cacgccggag
    31621 atggtttgca tcagcgcgcc gtagcgattt ttcagccctt cacggtacag cgttttaaag
    31681 cgtccggtgt tagaagtgcc atactgcgcc agttcgatgt cctgaccttc tgcgatgtag
    31741 catggcatac ttaacggcca catccgctca tcgcccatgt tgcgcgccgt atagcgatgc
    31801 aggtcgcgca taaaggtcag catatgttca atatcaccat ccactggtgt aataaattcc
    31861 agcaatgctt ccgcaaaatc ggtggttatc catttatgcg tcagtgcgga acctaatgct
    31921 tcaggatgac ctgttgttgc cagtgtgcca tcagcattaa cacgcaaagt ttcgcgctcc
    31981 agcccacgct gtatcccctt taacgcctga ggatgttttt ccagccaggc cagcgcctgt
    32041 gatacgtccg ggatcaaatt gacctcccgc ctgtcaaaat cgttttaatt agcataactg
    32101 taatggtgac catatgtgca ggcctacaat tagtgccacc acatcatgcc ctgaacggtc
    32161 gctgctgcaa ctgcaacata gcgtaacgct ttaccaaggc acaaaaaaaa gattaccggc
    32221 ccccaggaga tgcgcatcca tcccgctaac aggcacagta aatcgccaac tacgggcatc
    32281 cagcttaata atagcgtgac tgcaccatag cgtttcagcc agccagtagc tttctcttgc
    32341 cagcgcgatg ttttacgcaa tggaaagaaa cgcccaagga taacgttagt taaccctcca
    32401 aggctattac ccattgttgc tgttaaaact aaaacccagg gatgactgat cccggaaagc
    32461 aacgttgcca ccagcacgac ttcggagttg ccgggtaata gtgtagcgct gagaaaacta
    32521 ctggcaaaca acgagaaaag cgataacgct tcactcacag caagcgaaca tccacagcgt
    32581 ccatgcctgc tgcacgggcc gcctgaatac cgaaatcggc atcttcaaag accacacact
    32641 gcgtcggttg cacgcccata cgctgcgcgc acaacaaaaa tgtgtctggc gcgggtttat
    32701 ggtgtttgac gtgatcggca gcgacgacgg cgtcaaaata acggcgtaat cccaggtgag
    32761 ccagcaatgc ctcagcgatg gcgctttcac tccccgttcc tacagccatt gggcgacgac
    32821 catgccaact tttcaccact tcaacaagag gaagcggctc gacactatcc agcagcatac
    32881 ttcttactgc ttctgttttt tcacgcgcta acgcatgcgg gtcgagatcg gcctgattca
    32941 gctcaataat tgcctgagca atacgccagg tgggcgatcc attaagcgca atcatcgcct
    33001 gaacatcgta ctgaagaccg tagtgcccta atacttcgcg ccacgcttta cggtgcgtag
    33061 gctccgtatc caggattgtg ccatccatat caaaaattaa acctgcataa cgctcgtaca
    33121 tggtcttctc gcaagtcgaa aaatatgacg ctactttagc gtaattgctt gattttgtcg
    33181 ctgatggcga aggagaagaa aattcaggaa atggataaag tagttattta gaaatgagat
    33241 atttttgaag gaaatttttt ggagaagatg gtgcatccgg gaggattact cgccttgcgg
    33301 ctcgcccttc gggccgttgc taaagcaacg ttatcctccc tggtgcctgc ggttaatacc
    33361 gctacctctt cgatatcttc attgctgaag atttcggata atatggtgca tccgggagga
    33421 ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta tccagctgag ctacggatgc
    33481 atcgggaaac ttactttact gcagattttt tgataccgct actaaagccg tatcaagtaa
    33541 gagatggtgc atccgggagg attactcgcc ttgcggctcg cccttcgggc cgttgctaaa
    33601 gcaacgttat cctccctggt gcctgcggtt agtaccgcca cctcttccat accttcatcg
    33661 ctgaaggtta cggagaatat ggtgcatccg ggaggattcg aacctccgac cgctcggttc
    33721 gtagccgagt actctatcca gctgagctac ggatgcaaat ggcggtgagg cggggattcg
    33781 aaccccggat gcagcttttg accgcatact cccttagcag gggagcgcct tcagcctctc
    33841 ggccacctca ccacacgcct cttacgagtg cttcgaagaa cttgtttatc gctcatcgtc
    33901 gctgcgtggc gcacatatta ctttctgaga cttataagtc aaacaatttt tcccacactt
    33961 ttatcgtttg cacacttcac gttcaattag tctgtaaaaa cgtcaaaaag agtgttttat
    34021 caacagaaaa atggaggtct gacagatagc agtaatgtaa aaaaatggag acttaagttg
    34081 aatgaacggg agtaaagcga aaagactata gagtgaagga gaaattttga gggtgcgtct
    34141 caccgataaa gatgagacgc ggaaagatta gtaactggac tgctgggatt tttcagcctg
    34201 gatacgctgg tagatctctt cacggtgaac agaaacttcc ttcggggcat ttacgccaat
    34261 acgtacctgg ttgcccttta cccctaaaac tgtcacggtg acctcatccc caatcatgag
    34321 ggtctcacca actcgacgag tcagaatcag cattctttgc tccttgaaag attaaaagag
    34381 tcgggtctct ctgtatcccg gcattatcca tcatataacg ccaaaaagta agcaatgaca
    34441 aacacattac atctaagcag tcatggcatt acattctgtt aaacctaagt ttagccgata
    34501 tacacaactt caacctgact ttatcgttgt cgatagcgtt aatgcgaatg ccgtgaagcg
    34561 agtccacggc attgccttac gcttatatta ttgcaatttc gcgctgaccc agcctttcac
    34621 actggctaac gctgcaggta aggccgcagc atccgtacca ccggcttgcg ccatgtcagg
    34681 acgtccacca cccttgccac ccacctgctg agcgaccata ccaatcagtt cccctgcttt
    34741 cacacgatct gtgacgtcct tagatacgcc tgcaatcaga gaaaccttac cttcggctac
    34801 cgttgccagc acgataattg tcgaccccag ctgatttttt aaatcgtcaa ccatggtacg
    34861 caacattttc ggctcaacac cgctaagctc gctaaccaac agcttaacac cattaacatc
    34921 aattgcctta ctggaaagat ttgcgctctc ctgtgcggca gcttgttctt taagctgttg
    34981 tagttctttt tctaactgac gcgtacgttc cagtactgag cgcactttat cagccagatt
    35041 attgctatcg cctttcagca gatgtgcgac ttcgcttaag cgatcgctgt ctgcatgaac
    35101 ggtggcgata gcgccttctc cggttaccgc ttcaatacga cgaacgcctg cagcagtacc
    35161 cgattcagag atgatgcgga acagaccaat atcaccagtg cggctggcgt gagtaccgcc
    35221 acacagctcg gtggagaaat cgcccatgct cagcacgcgt acgcgctcat catacttctc
    35281 gccgaacagc gccatcgcac ctttcgcttt cgccgcttcg agatccatga tgttggtttc
    35341 gatcggcaaa ttgcgacgaa tctgtgcgtt caccaggtct tcgaccgcac gaatctcttc
    35401 tggtttcatc gcttcgttgt gtgagaagtc gaagcgcagc accttgtcgt taaccagtga
    35461 acctttctgc gatacatgag tacccagaac ctggcgcagc gcagcatgca tcaggtgcgt
    35521 tgcggagtga ttcagacgaa tacgggcgcg acgagcctca tcaacatcag cctgcacagc
    35581 gtcgcccact ttcagagaac ccgcagcaag tttaccgatg tgaccaatcg cctggccgta
    35641 tttctgcgta tcttccacca cgaaggagaa gttagcgcct ttcagttcgc ctttatcacc
    35701 aacctgaccg ccggattccg catagaatgg cgtttgatcc agcacgacca cagcttcctg
    35761 gcctgcattg atggcatcaa ccgctttacc atcaacaaac agcgcggtca ctttgccgtt
    35821 cagttccaga tggtcatagc ctttaaattc agatgcactg tcaacacgga tcattgcgtt
    35881 gtaatcggca ccaaagccgc tggcttcgcg cgcgcgacga cgctgctctt ccattgcagc
    35941 ttcaaaacca gcttcgtcaa ctttgatgtt gcgctcacga caaacatcag ccgtcaggtc
    36001 aaccgggaag ccataggtgt cgtacagacg gaaagcagtt tcaccatcca gcgtatcacc
    36061 agaaagtttt gccagctctt catccagcaa cgccagaccg cgctccagag tacgagcaaa
    36121 ctgctcttct tcagtcttca gcacctgctc aacctgcgcc tgctggcgtt tcaggtcttc
    36181 acccgcagag cccataacgt cgatcagcgg accaaccagt ttgtagaaga aggtttcttt
    36241 cgcgccgagc atattaccgt gacgcactgc gcgacgaatg atacgacgca gtacataacc
    36301 acggttttca ttggacggca ttacgccatc cgcgatcagg aacgcacaag aacgaatgtg
    36361 gtcagcgatt acgcgcagcg atttattgct cagatcggtt gcgccagtga ctttcgctac
    36421 cgcctggatc aacgtgcgga acaggtcgat gtcatagtta gagttaacgt gttgcagcac
    36481 cgcagcaata cgctccagac ccataccggt atctacagac ggcttcggca gcggttccat
    36541 cgtgccatcg gcctggcggt tgaactgcat gaagacgatg ttccagatct caatgtagcg
    36601 gtcgccgtct tcttccggac ttcccggagg gccaccccaa atgtggtcgc cgtgatcgta
    36661 gaagatttcg gtgcacgggc cgcacggacc agtgtcaccc atctgccaga agttgtcaga
    36721 tgcgtatggc gcacctttgt tatcgccgat gcgaataata cgttcgcgcg ggatcccgac
    36781 ttctttttcc cagatttcgt aggcttcgtc gtcgctttca tagacggtaa cccacagacg
    36841 ctctttcggc agggcaaacc atttttcgct ggtcagcagt tcccatgcaa actgaatggc
    36901 atcgtgtttg aaatagtcgc cgaagctgaa gttgcccagc atttcgaaga aggtatggtg
    36961 acgcgcggtg taaccgacgt tttccaggtc gttgtgttta ccacccgcac gcacacagcg
    37021 ttgggaagtg gtagcgcggg aataattacg cttgtcgagc ccaaggaaca catccttgaa
    37081 ctggttcatc ccggcgttgg taaacaacaa agttgggtcg ttatggggta ccagggagct
    37141 gctggcaact acctgatgtc ccttactatg gaaaaagtcg agaaacgcct gacggatctc
    37201 agcggtgctc ttgctcataa ttatcctgaa atcaagctaa cgaaatatcg ccaccagctc
    37261 cagcgtgtct taaccgccgg gctggtaact gaaaagtggg aataagataa gttttcttga
    37321 ctgggaagta aaataccgta tgcgttcagt cggcaaaatt tcgccaaata tcctggatat
    37381 cttccatcag atagccacga tagagcagaa aacgctggat cttaactttt tctgaaaaga
    37441 cagttgacaa aggttcacca tattttcgcg tcgcctgatc gcgcgccagt gcgcaccagt
    37501 cgatgtcaca ttcacgcatc gctttttctg tcgcttcgcg ggaaatacct ttctgattca
    37561 gttcctggcg aatacgcgca ggtccataac ctttgcggct acggctggcg ataaagcgcg
    37621 caacaaatcg gctgtcatcg agatagccat gttcatggca ccaggcaata acgcgctcgt
    37681 aatcttcttc cgtggcatca atctcttccg ggccattttt gcccataatc ggtgccgcga
    37741 gtttacgtcg cagttcttgc tcactgtgat cgcgcaccgc cagaatgcgt accgcacgat
    37801 ccaacaggcg agcatatgcc gggcgacggg acgttgattc tgtcatggca tatccttaca
    37861 acttaaaaaa gcaaaagggc cgcagatgcg acccaggttt tgctgataaa cagatttaat
    37921 agtcaatgag tgtgcagaat gttcccgcag gcccaattcg ttagaggatc gaggtgagag
    37981 taatttttca cgccagtgct ggcctgttcc cctcaccctc tccccaaagg ggcgagggga
    38041 ccgtccgtgc acacattatc tattatcatc aataacgaac gattaaaaat cttcgttagt
    38101 ttctgctacg ccttcgctat catctacaga gaaatccggc gttgagttcg ggttgctcag
    38161 cagcaactca cgtactttct tctcgatctc tttcgcggtt tccgggttat ctttcagcca
    38221 ggcagttgca ttcgctttac cctgaccgat cttctcacct ttgtagctgt accacgcgcc
    38281 tgctttctcg atcagcttct cttttacgcc caggtcaacc agttcgccgt agaagttgat
    38341 accttcgccg tagaggatct ggaattcagc ctgtttaaac ggcgcagcga ttttgttctt
    38401 caccactttc acgcgggttt cgctacccac cacgttttcg ccctctttca ccgcgccgat
    38461 acgacggatg tcgagacgaa cagaggcgta gaatttcagc gcgttaccac cggtagtggt
    38521 ttccgggtta ccgaacatca caccaatttt catacggatc tggttgatga agatcagcag
    38581 cgtgttggac tgcttcaggt tacccgccag cttacgcatc gcctggctca tcatacgtgc
    38641 cgcaaggccc atgtgagagt cgccgatttc gccttcgatt tccgctttcg gcgtcagtgc
    38701 cgccacggag tcaacgacga taacgtctac tgcgccagaa cgcgccaggg cgtcacagat
    38761 ttccagtgcc tgctcgccgg tgtccggctg ggagcacagc aggttgtcga tatcgacgcc
    38821 cagtttacgt gcgtagattg ggtccagcgc gtgttcagca tcgataaacg cacaggtttt
    38881 accttcacgc tgcgctgcgg cgatcacctg aagcgtcagc gtggttttac cggaagattc
    38941 cggtccgtag atttcgacga tacggcccat cggcagacca cctgccccaa gcgcgatatc
    39001 cagtgaaagc gaaccggtag agatggtttc cacatccatg gaacggtctt cacccaggcg
    39061 catgatggag cctttaccaa attgtttctc aatctggccc agtgctgccg ccaacgcttt
    39121 ctgtttgttt tcgtcgatag ccatttttac tcctgtcatg ccgggtaata ccggatagtc
    39181 aatatgttct gttgaagcaa ttatactgta tgctcataca gtatcaagtg ttttgtagaa
    39241 attgttgcca caaggtctgc aatgcatacg cagtagcctg acgacgcacc gcatcacggt
    39301 cgccgctgaa gcattcccgc cgggtaatgc cttcaccgcg ggcagtggca aaagcaaacc
    39361 agacggtgcc gacaggcttc tcttcactgc cgccatccgg cccggcgata ccactaatag
    39421 acacggcgta atcagcacga gccgctttca gtgcgcctat cgccatttcc accacgacgg
    39481 gttcgctcac cgcgccatgc tgcgccagcg tctcttcacg tacgccgatc atctgcgctt
    39541 tggcttcgtt actgtaggtg acaaatccgc gttcaaacca ggcggagcta ccggcaatat
    39601 cggtaatcac tttcgctacc caaccgccgg tacaagactc agcagttgtt acggttgcgc
    39661 cacgggcttt cagcgcctgc ccaacctgtt cgcttaactg catcagttca ctgtcagtca
    39721 tcacaacctc tgtcagtcaa aaatttatgc cggacaagat agcactttcg tcaggaagat
    39781 ggtgttggcg ttgcaatttt acgaggagga ttcagaaaaa agctgattag ccagagggaa
    39841 gctcacgctc ccctcttgca aatagttact gcacgcgcgc cagcgccacg gcttgtccta
    39901 actgccagac cgccattgcg taatgggtgc tgtggttata acgggtaatg gtgtagaagt
    39961 tcggcagacc gtaccagtac tggtaaccgg taccgacatc cagacgcagc aggctggctt
    40021 gttgatggtt gcccagcggc tgctgtggcg ttaaacctgc cgcggcaagc tgcgaaatgc
    40081 tgtacttggt tttgaagcca tttggcaagc ctggagcctg accgtttgcc attaccgcga
    40141 cctgatcgcc tttcacccaa ccgtgcgctt tgaaataatt cgccacgcta ccgatcgcat
    40201 caaccggatc ccacaggttg atatgcccgt cgccgctgaa atctaccgca tattgtttgt
    40261 aagacgacgg cataaactgt ccgtagccca tcgccccggc aaaggaacct ttcagattga
    40321 gcggatcgtc ctgctcgtcg cgcgccatca gcaggaaggt ttccagctcg ccagagaaat
    40381 actccgcgcg gcgtgggtag ttaaatgaca gcgttgccag cgcatcgagg atgcgagttt
    40441 tccccatcac gcgcccccag cgggtttcaa cgccgataat cccgacgata atttccggcg
    40501 gtacgccata cacctgccac gcgcgattca acgcatcttc atactgattc cagaaaacca
    40561 caccgttctg cacgttgtcc ggcgtaataa attttttgcg ataacgcagc catgcgccgt
    40621 tcggacctga tggcggtttc accgatgtgg ttggtgcctg gttatccatc agccgcagta
    40681 ccgaatccag acgtttcgcc tgggagagaa tttcctgcaa ctgctgacga tcgaaaccgt
    40741 gtttgttcac cattttgtcg atgaactgct gggcattcgg gttattggcg aagtcaccgc
    40801 ccatctgcat cacattgtgc tgcggctcaa gcaggaagcc gccagacggc gttccggtgg
    40861 tcgtttcagt ctcagtaggt tttggcttgc tgctacaggc ggcaagcaac acaaaaaggg
    40921 gaagcaatgt tacataacga cgcttgaaca tgaggggtcc atttaacaga ttcaaccagg
    40981 ggcaagtatg gtaaagcatc acgccccgca caaggaagcg gtagtcactg cccgatacgg
    41041 actttacata actcaactca tccccctcgc tatcctt
//
LOCUS       DAZFKF010000036        40841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFKF010000036 DAZFKF010000000
VERSION     DAZFKF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.036"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(56..337)
                     /gene="yehE"
                     /locus_tag="ACHKA1_002334"
     CDS             complement(56..337)
                     /gene="yehE"
                     /locus_tag="ACHKA1_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO5679976.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(599..1708)
                     /gene="apbC"
                     /locus_tag="ACHKA1_002335"
     CDS             complement(599..1708)
                     /gene="apbC"
                     /locus_tag="ACHKA1_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO5679977.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1840..3873
                     /gene="metG"
                     /locus_tag="ACHKA1_002336"
     CDS             1840..3873
                     /gene="metG"
                     /locus_tag="ACHKA1_002336"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO5679978.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4014..4208
                     /locus_tag="ACHKA1_002337"
                     /pseudo
     CDS             4014..4208
                     /locus_tag="ACHKA1_002337"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            6741..7817
                     /locus_tag="ACHKA1_002338"
                     /pseudo
     CDS             6741..7817
                     /locus_tag="ACHKA1_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7830..10862
                     /locus_tag="ACHKA1_002339"
     CDS             7830..10862
                     /locus_tag="ACHKA1_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO5679979.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQENELHITWGKVGSNGQSQVKSFAD
                     ATAAEKAELKLIAEKVKKGYVEQVDANAHSHDASAKKVLVTEEKASINRQAATNGLPW
                     LADNDPIILPPNIARHALSHRLWPGEPVKKPTKPEKQNLISRLANRTYHYYKNLTFDY
                     SSCSDEWADAISQAISLIKAQKTEIPARTMAVLTFLEICFCQGWNNETSSELLDEIVS
                     TFGIEYATETVIFWWQIDFDFDYKDELLTFFINYADSSKDSYRRNNDKFSLRLRKHLS
                     LAEEEVWQNCIAKLLVALPDISLFRQPLIAILMPEIPEVAHEIVHRLHQVADVPQLEM
                     LKLVATDPFTLEILENYQYIDVFNTYGASWSATVLREQGIAALARLAPYAEEDNCGDV
                     LKCINHPLAITALIEAADTNKRCYERLIKSAENFPSATIAALAEALVKKDDKRKHTLL
                     VSLLNANPLLVEKLVIGLSSQAANLLQQCQQLLNSRNEDFANSEILPMVLVSPPWETQ
                     NKNNDNSPVGKYPAKIPPLPLFYQPPLWTRPKIKNSSKVLPDSALQHLGEMLRFPQEE
                     ALYPGLLQVKDACTADSLAEFAWDLFTAWLTAGAPSKESWAFTALGVLGNDDTARKLT
                     PLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIAQKLKFKALQERAKEKIADIA
                     ESRELTVAELEDRLAPDLGLDDNGSLLLDFGSRQFTVSFDETLKPFVRDVSGSRLKDL
                     PKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVARLESAMCLRRRWSPENFQLFLVE
                     HPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTADDDLFTLPEGDISIGIPHVLE
                     ISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERNASELTRWAGRKCPSGRVMGL
                     ANKGWIKGTPQDGGWIGWMIKPLGRWSLIMEIDEGFAVGMSPAELSAEQLLSKLWLWE
                     GKAESYGWGSNSTQEAQLSVLDAITASELINDIEALFE"
     gene            10872..14504
                     /gene="yehI"
                     /locus_tag="ACHKA1_002340"
     CDS             10872..14504
                     /gene="yehI"
                     /locus_tag="ACHKA1_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO5679980.1"
                     /translation="MDKELPWLADNAQVELKYKKGKTPLSHRNWPGEPVPVITESLIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSVPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEKSCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQKNRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNNPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLKSYLSALPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLASTESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLM
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAVQRDLARQWILHWPEHTATALIPL
                     VFTKSSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEHLLKQSPIEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQTVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRHFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHLLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            14565..14882
                     /gene="yehK"
                     /locus_tag="ACHKA1_002341"
     CDS             14565..14882
                     /gene="yehK"
                     /locus_tag="ACHKA1_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO5679981.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLHPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            16122..17210
                     /gene="yehL"
                     /locus_tag="ACHKA1_002342"
     CDS             16122..17210
                     /gene="yehL"
                     /locus_tag="ACHKA1_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO5679982.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDAAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            17221..19500
                     /gene="yehM"
                     /locus_tag="ACHKA1_002343"
     CDS             17221..19500
                     /gene="yehM"
                     /locus_tag="ACHKA1_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO5679983.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFLGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQSWCWQWGLQQAGEQLLKT
                     VLTRLRQHHLPASTADMAAAHLHAMALAQLRGHKLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESDGKPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHVPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLN
                     DDDFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            19493..20629
                     /gene="yehP"
                     /locus_tag="ACHKA1_002344"
     CDS             19493..20629
                     /gene="yehP"
                     /locus_tag="ACHKA1_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO5679984.1"
                     /translation="MSELNDLLTNRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSSIPLARNFDFKSTLRANLQHWHPQHSKLYIESPRFNSRIKRHSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVNLTADVADPVELLMKVQ
                     LGGGTSIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            20626..22629
                     /gene="yehQ"
                     /locus_tag="ACHKA1_002345"
     CDS             20626..22629
                     /gene="yehQ"
                     /locus_tag="ACHKA1_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO5679985.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMAQRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWRMLSAPLRQPGIVALR
                     EYLRQRPPSCIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSAGVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHMLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            22754..23215
                     /gene="yehR"
                     /locus_tag="ACHKA1_002346"
     CDS             22754..23215
                     /gene="yehR"
                     /locus_tag="ACHKA1_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO5679986.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(23256..23726)
                     /gene="yehS"
                     /locus_tag="ACHKA1_002347"
     CDS             complement(23256..23726)
                     /gene="yehS"
                     /locus_tag="ACHKA1_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO5679987.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(23773..24492)
                     /gene="btsR"
                     /locus_tag="ACHKA1_002348"
                     /gene_synonym="yehT"
     CDS             complement(23773..24492)
                     /gene="btsR"
                     /locus_tag="ACHKA1_002348"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO5679988.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(24489..26174)
                     /gene="btsS"
                     /locus_tag="ACHKA1_002349"
     CDS             complement(24489..26174)
                     /gene="btsS"
                     /locus_tag="ACHKA1_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO5679989.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            26396..27127
                     /gene="mlrA"
                     /locus_tag="ACHKA1_002350"
     CDS             26396..27127
                     /gene="mlrA"
                     /locus_tag="ACHKA1_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO5679990.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGYDIFPLGI"
     gene            27187..27294
                     /gene="yohO"
                     /locus_tag="ACHKA1_002351"
     CDS             27187..27294
                     /gene="yohO"
                     /locus_tag="ACHKA1_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO5679991.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(27275..28006)
                     /gene="yehW"
                     /locus_tag="ACHKA1_002352"
     CDS             complement(27275..28006)
                     /gene="yehW"
                     /locus_tag="ACHKA1_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO5679992.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(28011..28937)
                     /gene="yehX"
                     /locus_tag="ACHKA1_002353"
     CDS             complement(28011..28937)
                     /gene="yehX"
                     /locus_tag="ACHKA1_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO5679993.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVVDYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(28930..30087)
                     /gene="yehY"
                     /locus_tag="ACHKA1_002354"
     CDS             complement(28930..30087)
                     /gene="yehY"
                     /locus_tag="ACHKA1_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO5679994.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKFGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(30094..31011)
                     /gene="osmF"
                     /locus_tag="ACHKA1_002355"
     CDS             complement(30094..31011)
                     /gene="osmF"
                     /locus_tag="ACHKA1_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO5679995.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLGRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(31263..33560)
                     /gene="bglX"
                     /locus_tag="ACHKA1_002356"
     CDS             complement(31263..33560)
                     /gene="bglX"
                     /locus_tag="ACHKA1_002356"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO5679996.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSSQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            33756..35471
                     /gene="dld"
                     /locus_tag="ACHKA1_002357"
     CDS             33756..35471
                     /gene="dld"
                     /locus_tag="ACHKA1_002357"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO5679997.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(35510..36442)
                     /gene="pbpG"
                     /locus_tag="ACHKA1_002358"
     CDS             complement(35510..36442)
                     /gene="pbpG"
                     /locus_tag="ACHKA1_002358"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO5679998.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(36616..37203)
                     /gene="yohC"
                     /locus_tag="ACHKA1_002359"
     CDS             complement(36616..37203)
                     /gene="yohC"
                     /locus_tag="ACHKA1_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO5679999.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            37373..37951
                     /gene="yohD"
                     /locus_tag="ACHKA1_002360"
     CDS             37373..37951
                     /gene="yohD"
                     /locus_tag="ACHKA1_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5680000.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKIIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(38081..38842)
                     /gene="yohF"
                     /locus_tag="ACHKA1_002361"
     CDS             complement(38081..38842)
                     /gene="yohF"
                     /locus_tag="ACHKA1_002361"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5680001.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(38895..40331)
                     /gene="mdtQ"
                     /locus_tag="ACHKA1_002362"
     CDS             complement(38895..40331)
                     /gene="mdtQ"
                     /locus_tag="ACHKA1_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO5680002.1"
                     /translation="MNRDSFYPAIACFPLLLMLTGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSELFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            40555..40638
                     /gene="yohP"
                     /locus_tag="ACHKA1_002363"
     CDS             40555..40638
                     /gene="yohP"
                     /locus_tag="ACHKA1_002363"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO5680003.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT        10214 a        10731 c        10204 g         9692 t
ORIGIN      
        1 tcatttatat aaatgatttc ggctttctaa atgcaattaa aatcaggttg ccaaattaat
       61 cgtagcgatt taacacgatt cgacgtttgc tatcactacc tgcaacaacc acttctgtcg
      121 ttacaccttt gacgggcgaa gaaacctgat gacgggtatc gacattatac gttaactgac
      181 cacaatgctg ttgaggttga ctattaacca tagccatact gcatgttggg ggactgatcc
      241 tgccattaat cgttaatgtg gcagtctctg aagagaacgc gggagaagcc aatccatagg
      301 caagaaaaat aattcctgac agccaatact tattcattga acgttatccc tgtagtaaag
      361 gttatgcctg gatagaatga gtgcataaca aactatagct gtacatccac tacacagcac
      421 gaaggatgat aatgaagcat tgcctgtacg atcaatcgac tttgtagagt ttcggacgaa
      481 ggtccgcaga atattcgcag tattaaataa gtgttcatta atgcggcaaa aacaaataca
      541 ccgcatcaat ataacatctc tgcggtgtat gaagaataac cagaatggtt aataggcgtt
      601 agaccgcgcg gaaggaaatc tcgcctggaa tgacttcacc ctgccagtag agctgagctg
      661 caacgcggtc agccagctgg cggtagatag cggtaaattc gctctccgga cggctgataa
      721 ccgttggcgt gcctttatcg agatcctcac gtaaggagat gtgtaacggc atctgcccca
      781 gcagctgtgt atggtatttt tcagccagtt tctctgcacc accggtaccg aaaatcggtt
      841 catggtgacc acagttactg caaatatgca cactcatgtt ctcgacaata ccgagcaccg
      901 gaacctcgac tttctcgaac atcacaatgc cttttttcgc atcgatcaac gcgatgtcct
      961 gcggggtggt gaccacgact gcaccagtta ctggaatgtt ttgcgccagc gtcaattgaa
     1021 tgtcaccggt gcctggcggc atatcaagca ccagatagtc gagatccggc cacaaggttt
     1081 cctgcaacat ctgcattaac gccttgctcg ccatcggtcc acgccacacc atcgcattgt
     1141 cgtcagtgac cagataacca attgaattgg ttgccaggcc atgagacata atcggtgcca
     1201 tatgggtacc gtccggcgaa gttggacgct gattttctgc gcccagcatg gtgggaattg
     1261 atgggccgta gatatcggcg tccagaatgc cgactttcgc cccttccgcc gccagtgcca
     1321 gcgccaggtt taccgccgtg gaggatttac ccacgccgcc tttaccggaa ctgaccgcaa
     1381 tgatgttctt cacgccatta atacccggct gattcttcac gcgttttaag gtggcgatat
     1441 tgtgcgacag tttccagtcg atagctttcg ccccggtgat acgcagcagt tcggcgctac
     1501 attgctcttt taactcttca aacgcgctat gccagacgaa tggcataacc agttccacat
     1561 gcaacgtgtc gtccatccag gcgacatggt gcaacgcttt cagcgtggtg aggttatgct
     1621 tcagggttgg gtgctgaaaa ttggccagtg tcccggcgac cattgcgcgc aaggcttctg
     1681 gtgatttggc ctgggattgt tcgttcatcc cgactccttt tttgtataga taaaccatca
     1741 gctgatagtt tacctgaaga atatagagaa gtacttactt aacattttcc catttggtac
     1801 tatctaaccc cttttcacta ttaagaagta atgcctacta tgactcaagt cgcgaagaaa
     1861 attctggtga cgtgcgcact gccgtacgct aacggctcaa tccacctcgg ccatatgctg
     1921 gagcacatcc aggctgatgt ctgggtccgt taccagcgaa tgcgcggcca cgaggtcaac
     1981 ttcatctgcg ccgacgatgc ccacggtaca ccgatcatgc tgaaagctca gcagcttggt
     2041 atcaccccgg agcagatgat tggcgaaatg agtcaggagc atcagactga tttcgcaggc
     2101 tttaacatca gctatgacaa ctatcactcg acgcacagcg aagagaaccg ccagttgtct
     2161 gagcttatct atactcgctt gaaagaaaac ggttttatta aaaaccgcac catctctcag
     2221 ctgtacgatc cggaaaaagg catgttcctg ccggaccgtt ttgtgaaagg cacctgcccg
     2281 aaatgtaaat ccccggatca atacggcgat aactgcgaag tctgcggcgc gacctacagc
     2341 ccgactgaac tgatcgagcc gaaatcggtg gtttctggcg ctaccccggt aatgcgtgat
     2401 tctgaacact tcttctttga tctgccctct ttcagcgaaa tgttgcaggc atggacccgc
     2461 agcggtgcgt tgcaggagca ggtggcaaac aaaatgcagg agtggtttga atctggcctg
     2521 caacagtggg atatctcccg cgacgcaccg tacttcggtt ttgaaattcc gaacgcgccg
     2581 ggcaaatatt tctacgtctg gctggacgca ccgattggct acatgggttc tttcaagaat
     2641 ctgtgcgata agcgcggcga cagcgtaagc ttcgatgaat actggaagaa agactccacc
     2701 gccgagctgt accacttcat cggtaaagat attgtttact tccacagcct gttctggcct
     2761 gcaatgctgg aaggcagcaa cttccgcaag ccgaccaact tgtttgttca cggctatgtg
     2821 acggtgaacg gcgcgaagat gtccaagtct cgcggtacct ttattaaagc cagcacctgg
     2881 ctgaatcatt ttgacgccga cagcctgcgt tactactaca ctgcgaaact ctcttcgcgc
     2941 attgatgata tcgatctcaa cctggaagat ttcgttcagc gcgtgaatgc cgatatcgtt
     3001 aacaaagtgg ttaacctggc ctctcgtaat gcgggcttta tcaacaagcg ttttgacggt
     3061 gtgctggcaa gcgaactggc tgacccgcag ttgtacaaaa ccttcactga tgccgctgaa
     3121 gtgattggtg aagcgtggga aagccgcgaa tttggtaaag cagtgcgcga aatcatggcg
     3181 ctggctgatc tggctaaccg ctatgtcgat gaacaggctc cgtgggtggt ggcaaaacag
     3241 gaaggccgcg atgctgacct gcaggcgatt tgctcaatgg gcatcaacct gttccgcgtg
     3301 ctgatgactt acctgaagcc ggtactgccg aaactgaccg agcgcgcaga agcattccta
     3361 aatacggaac tgacctggga tggtatccag caaccgctgc tgggccacaa agtgaatccg
     3421 ttcaaggcgc tgtataaccg catcgatatg aagcaggttg aagcactggt ggaagcgtct
     3481 aaagaagaag tgaaagcagc tgccgcgcca gttactggcc cactggcaga tgatccgatt
     3541 caggaaacca tcacctttga cgacttcgcc aaagttgacc tgcgcgtggc gctgattgaa
     3601 aacgcagagt ttgttgaagg ttctgacaaa ctgctacgcc tgacgctgga tctcggcggt
     3661 gaaaaacgca atgtcttctc cggcattcgt tctgcttacc cggatccgca ggcactgatt
     3721 ggccgtcaca ccattatggt ggctaacctg gcaccacgta aaatgcgctt cggtatctct
     3781 gaaggcatgg tgatggctgc cggtcctggc gggaaagata ttttcctgct aagcccggat
     3841 gccggtgcta aaccgggtca tcaggtgaaa taatccccct tcaaggcgct gcatcgacag
     3901 cgccttttct ttataaattc ctaaagttgt tttcttgttt gttcaacgct cttaattttt
     3961 ttcattaact gctagcatcc gcttataact ggatataaat acagggaacg cggatgaaga
     4021 gttatatcta tcaggacgaa aaatcacata aattctgggc ggtggagcag cagggaaacg
     4081 agttgcatat cagctgggga aaagttggta cccaagggca aagccaggta aaaaattttg
     4141 ctgatgttgc agcagcggaa aaagcgaaac tgaagcttat cgcggagaaa acgaaaaaag
     4201 gctatgtgga aaatgcttca gcaaacgtgc atatcccccc cattaccaaa gccactcctg
     4261 aagttgaaac ttcccctgag agtaaaaacc aacgcccctg gctggctgat gatgcggtca
     4321 ttcggctaac tgacgatatt aatcgatttg cttttcctca tcgctctcgc cccagagaga
     4381 tcaattatct gcacaaagat ggtcaaatat ggcagctcat cgcgaataac accaaggcat
     4441 atgatcccgc caacaattac cgttcttatc cagaaaactg gcagcaggct tttgctgagt
     4501 tacaaatgcg agttcagggt aatcaacaga cagggagtgt gcaatctgat gcagcattgc
     4561 tctggagttt ctggaactct tactctgcag atgaactgat cgatgactta gtcattcgct
     4621 gtggtctgga aagtgccgtt gaaattgccc ttctcgctct tcaacttaga tataaaccag
     4681 taaaagccga cgtcaccacc atcattcccc ctgatctcaa agcagaatcg ctaccgaact
     4741 ggcatcaacg tctatgtcat catctttcac tcgcttcaga agatgagtgg caacgctgtg
     4801 tagataaggt actcgcggct attccctcgc tatccccagc acggcaacct tttgcggcgt
     4861 tactgatccc tgaacgcccg gatatcgcca atgctatggc gctacattat gcagatcaaa
     4921 acgttccggc gatgacctgg ttaagtatga tggtaagcga tgatgttgcc ctggccaccc
     4981 tggaaaaata tggtttccca ccgctatata atgattttcg caaatatctg gctacgttgc
     5041 tggcaaataa tggaatgcgt ggtgtaagcc ggatacttct caagctacct gtcgactatc
     5101 ccgtaaaata cacggacctt ttcactcaca tacacgccaa tgctgaagat cttgtcaaat
     5161 ggctatggaa aacgaatcac ccagatgcga ttcaaattct gatcctcggt gtaaatggca
     5221 agaaaaagca cctggaatac ttaagcaaag cctgccaaaa acatcccgct gcggctattg
     5281 cggcttatgc tactttgctg gcaatacatg aagataaaga gtggcgtaaa gcgcttgtca
     5341 aactgattac cgccacgcca gagttagtat gcgacgtcat tccctgggtt aatgctaaag
     5401 cggcaggcat cttatctgaa tgtcgtccgc agccagtagc tgaagaatgt gaatatgcca
     5461 ctgtggatat gttgccggag ctgcttgttt cgccgccctg gatgaccaaa gagaaaaaga
     5521 aaaatacgcc tgtgtttgat cttccagtgc ttccagttcc ttctgtttct gatgtcactc
     5581 cagagattac caaaaaactg acccgtactt accttgtcac ccacttccag caaatagccc
     5641 aacagcaggc gacaaaacaa acattgttta ccgacctgcc gccgatcaaa aaagcaagct
     5701 gggagaaaca cttaataccg cttacaccgg agcaacaaat actgtggcat cttggttttg
     5761 aaaaatggcg ggaaagcgga gaaaaaatat acgaaaaaat tccggcgcca caaagcgccg
     5821 ttgatgccct gctacgcttc gattttccag cactcaatgc tgaatttgtg caatatcata
     5881 acaatgcata caaaagctgg aatctcattg ccctctgtta cctgcctggt caacaggcca
     5941 tttcttttct taaccagata gtcaaagaag ataactactc tggtgaggga aatattctgg
     6001 caatttttgg tagcgccgcc atacctgcat ttatggcctg cctgcaaggt gatccacgac
     6061 gtctgtgctt cttccccttt ttcctcggtg tcagtgaact ggccttaccg atggcgcaac
     6121 aattacagaa aaaaatgtct tatgaagatg cgcgaaactg gctaactgat tatccacgcc
     6181 atgccgccgc cgggctactt cctgtggccc tgggcaaaaa aggaaaagat cgtgattgtg
     6241 cccgccaggc gcttcgtctg ctggtgaatt taaaccagcg agaaacgatt gaggaaatcg
     6301 cgcaaggata taatcaacct gatgttttag ctgcgctggc aacattgttc gatagcgacc
     6361 cactggaaga atatcccgct aaaatcgccc cactgtccgg cttttatcag ttcaccttgt
     6421 ggcgcagacc acggcttaaa agtaacaacc tgcctctgtc agatgacgct atgcgccacc
     6481 tcggcactat gctgagcttt cctcgcgaca ttaccgccta cgctgggctg gatatcatta
     6541 gagaaatctt cacccgcgaa tcactggctg aatttggctg ggatctgtat accgcctgga
     6601 cagaagctgg cgcacccgca aaagaaaact gggcatttac ttcgcttggg attttaggca
     6661 atgacgacac cgcgcgtaaa ttaaccccgc ttatccgcgc ctggcctggg gaatcccagc
     6721 ataaacgggc ggtgtccggg ctggatgtat tagctgatat tggcagcgat gttgcgctaa
     6781 tgctgcttaa tggcatcgcg cgaaaaatta aattcaaagc attacaggaa catgcccgcg
     6841 agaaaatcaa catagttgct gaaaaccgtg ggctgactat ggctgaactg gaagaccgcc
     6901 tggctccaga tttagggctt gatagcagtg gctcgctgat actggatttc ggcccccgca
     6961 aattcaccgt tggttttgac gaaaccttaa aacctgtagt gtgcgatgca aacggcaaag
     7021 tcctgaaaga tttacctaag ccaaaccaga gcgatgataa aacactggca actgacgcag
     7081 ttaatctctt caaacagttg aaaaaagatg ttcgtgctat cgccagccag caaattaccc
     7141 gtctggaaca ggctatgtgc cagcgcagac gctggacggc agagcagttc cgcctgtttc
     7201 tggtggagca tccgctggta cgtcacttaa cccgacggct gttatggggg gtttataacg
     7261 atgaaaacgc tcttatcacc tgttttcgcg tggcagaaga cagcacttac agcgatgcgc
     7321 aggatgagtt attcacgctg ccagcaggaa acatcggtat tccgcatgtg ctggaaatat
     7381 cccctgaatc cgccgctgca ttcaggcaaa tttacgctga ttacgaactg cttccccctt
     7441 tccaacagct cgagcgaggt agctatcacc ttgctgataa tgaacgtaat actcacgaac
     7501 tgacacgctg gcaaggacgg ctttgccagg ccggacgcat tgtcgggctg gaacgcagag
     7561 gctggcaacg tctggaagaa agcggcagcg tttatgcgat gcgcaaaaca acccctcatg
     7621 gcgatctcga acttgaaacg gaaccttttt cattaattta tggtgaaacg gggtatggcg
     7681 accagctacc ggttgaaagc gtaaaaatca cctcgccaga tgatcgctac ggtaaacaat
     7741 cctcactcac tttctccatg ctcgacgaca tcaccgccag cgagctgatt aacgacatcg
     7801 aatcactgtt tgattaaaag gatttaacaa tgaaaaccta tatttatcag gacgaaaaat
     7861 cacataaatt ctgggcggtg gagcagcagg aaaacgagct gcatattacc tggggtaaag
     7921 tcggtagtaa cgggcaaagt caggtaaaaa gctttgctga tgctacggcg gcggaaaaag
     7981 cggaactgaa gctgattgcg gagaaggtga agaaggggta tgtggagcaa gtagacgcta
     8041 acgctcactc acatgatgcc tcagcaaaga aagtcctggt cacagaggaa aaagcctcaa
     8101 taaatcgcca ggcagcaact aatggtttac cctggctggc agacaacgat cccattattc
     8161 ttcccccgaa tattgcccgc catgccctca gccaccgcct ttggccggga gagccagtaa
     8221 aaaaaccaac caaacccgag aaacaaaatt taatttcccg attggcaaat cgtacttatc
     8281 attattataa aaatctgact ttcgattaca gctcatgctc tgatgaatgg gctgatgcga
     8341 ttagccaggc aatcagctta attaaggccc aaaagactga gatacccgcg cgtactatgg
     8401 cggtacttac ttttctggaa atatgttttt gtcagggctg gaataatgaa acctcatccg
     8461 agttgctgga tgaaattgta agcaccttcg gtatcgaata cgcaactgaa actgtcatct
     8521 tctggtggca aattgatttc gattttgact ataaagatga acttttgact ttctttatta
     8581 attatgcaga tagcagcaag gacagttata gaaggaataa cgacaaattt agtttacgtc
     8641 tgcgtaaaca tttatcacta gcagaagaag aagtatggca aaattgcatt gcaaaattac
     8701 tcgttgcgtt accagatata tcactctttc gccaaccgtt aattgccatt ttaatgccag
     8761 aaatacccga agtggcccat gagatcgtac atcgtttaca ccaggtcgca gatgttccac
     8821 aacttgaaat gttgaaactg gtagcgacag atccattcac gctcgaaata ctggagaact
     8881 atcaatacat tgatgtattt aacacctatg gtgccagttg gtcagctacg gtattacggg
     8941 agcaaggtat agcggcactg gctcgcctgg ctccctatgc tgaagaagat aattgcggtg
     9001 acgtactgaa atgtattaat catccgttgg ccattacggc actcatcgaa gccgccgata
     9061 ctaataaacg ctgttatgaa cgactaataa aaagtgccga aaattttcct tctgccacga
     9121 tagcggcatt agccgaagca ttggtaaaaa aagatgataa acgcaaacat acgttgctgg
     9181 tttcattact taatgcaaat cctttattgg ttgaaaaatt agtcatcgga ttatcgtcgc
     9241 aagcggcaaa tttattgcaa caatgtcagc aattgctgaa tagtcgcaat gaagattttg
     9301 caaactcaga aattctacca atggtactag tctcgccgcc ctgggaaact caaaataaaa
     9361 ataacgacaa cagtcctgtg ggtaaatacc cggcgaaaat ccccccttta ccgttgtttt
     9421 atcaacctcc attatggact cgcccgaaaa tcaaaaatag cagcaaggta ctgccagata
     9481 gcgcactcca gcacctcggt gaaatgctcc gcttccctca ggaagaggcc ctgtatccgg
     9541 gattattgca ggtgaaagat gcctgtaccg ccgactcact ggctgaattt gcctgggatc
     9601 tgtttaccgc ctggttgact gcgggcgcac cgtcgaaaga gagttgggcg ttcactgcgt
     9661 taggcgttct tggtaacgat gacaccgccc gcaaactgac gccattaata cgcgcctggc
     9721 ctggtgaatc ccagcataaa cgcgccaccg ttgggttgga tattctcgcc gctatcggta
     9781 gtgatatcgc cctgatgcag cttaacggta ttgcccagaa actgaaattc aaagcattac
     9841 aggagcgggc aaaagaaaaa attgccgaca ttgccgaaag ccgcgaactc acggtggcag
     9901 agcttgaaga tcggttagca ccggatctcg gtctggatga taacggttcg ttgctgctgg
     9961 atttcggctc acgccagttc accgtcagct ttgatgaaac cttaaaaccg tttgtgcgtg
    10021 atgtttctgg cagccgtctg aaagacctgc ccaaaccaaa caaaagcgat gatgaatcac
    10081 aggcgaacga tgctgttaac cgctacaaat tgctgaaaaa agatgcgcgt accgtcgccg
    10141 cccagcaggt agcaaggctg gaatccgcca tgtgcctgcg ccgccgctgg tcgccggaaa
    10201 acttccagct cttcctggtt gagcatccgt tggttcgcca cttaacccgc cgtctgattt
    10261 ggggtgttta tagcgccgaa aaccagctac tggcttgctt tcgcgtagcg gaagataaca
    10321 gctacagtac cgctgacgat gatcttttca ccctgccgga aggcgatatc tctatcggca
    10381 ttcctcacgt tctggaaata tcaccaacgg atgctgccgc ctttggtcag ctttttgccg
    10441 actacgaact gctaccaccg ttccgtcagc ttgaccgtaa cagttacgcc ctgacagaag
    10501 ccgagcgcaa cgccagcgaa ctgacccgct gggcaggcag aaaatgcccg agcggtcggg
    10561 tcatggggct ggcgaataaa ggctggataa agggcacccc gcaggatgga ggctggatcg
    10621 gctggatgat caaacctctg ggccgctggt cgttaatcat ggaaatcgat gaaggttttg
    10681 cggtaggcat gtcgccagcc gaactgagtg ccgagcagct cttaagcaag ctgtggctat
    10741 gggaaggcaa agcagaaagc tatggctggg ggagtaattc aacacaggaa gcgcagctct
    10801 ccgtactcga tgccatcacc gccagcgagc tgattaacga tattgaagct ctgtttgaat
    10861 aaggaaagtg catggacaag gaattaccgt ggctggcgga taacgcccaa gtggaactga
    10921 aatataaaaa aggcaaaaca ccactcagcc atcgcaattg gccgggcgaa ccggtgcctg
    10981 ttatcactga aagcctcatc cagacactgg gtgatgaatt gctacaaaaa gcagagaaga
    11041 aaaaaaacat tgtctggcgt tatgaaaatt tttcactgga gtggcagtcc gccattacgc
    11101 aggccatcaa cttgatcggc gaacacaaac cctcagtccc ggcccgcaca atggcggcgc
    11161 tagcctgtat cgcgcaaaat gacagccaac agttgctcga cgaaatcgtc caacaagagg
    11221 ggctggaata tgcgactgag gtggtgattg cacgccagtt tattgcacgg tgttatgaga
    11281 gtgatcctct ggtagtcaca ttgcagtatc agaacgagga ctatggctat ggttatcgct
    11341 cagaaaccta taacgaattc gatctccgac tgcgtaaaca cctctctctg gcagagaaaa
    11401 gctgctggca gcgttgcgcc gacaaactca ttgccgcact gccaggaata accaaagttc
    11461 gccgtccttt tattgcgctg atcctcccgg aaaagcctga aatagccaat gaactcgcca
    11521 gtcttgaaag ctcccggtca agtctccatt caaaggagtg gttaaaggtc gttgctactg
    11581 ataatacggc ggtaaaaaaa ctcgaacgat actggggcct ggacgtattt agcgatcgcg
    11641 aagccagcta catgtcacaa aaaaaccgct tcggctacgc ggcctgcgcc tcccttttgc
    11701 gcgaacaagg actggcagcc gttccgcgcc tggcaatgta tgctcataaa gaagattgcg
    11761 gcagtctgct ggtacaaatt aaccatccgc aagtcatccg caccttgctg ttagttgctg
    11821 ataaaaacaa ccccagcctg caacgtgtgg ctaaatacag taaaaacttc ccccatgcga
    11881 cgctcgccgc actggcagaa ctgctggcgt taaaagaacc accagcccgc cctggttatc
    11941 caatcatcga agacaaaaag ctgcctgcac agcaaaaagc tcgagatgaa tactggcgta
    12001 cgctgttaca aacgctgatg gcatcgcagc cacaactggc agcagaagtg atgccgtggt
    12061 taagtactca agccagggca gtgctgaaga gttatttatc ggcactgcca aaaccggtta
    12121 ttgatggcac cgataacagc aatctgcctg aaatcctcgt ttcaccaccg tggcgtagta
    12181 agaaaaaaat gacagctcca cgtcttgatt tggcaccgct cgaattaacc ccgcaagttt
    12241 actggcaacc aggcgaacaa gagaggcttg cctccactga gtctgcccgt tatttcagca
    12301 cggaatctct tgcggaacgc atggaacaaa aaagtgggcg agttgtatta caggaactgg
    12361 gttttgggga tgatgtatgg ctgtttctga attatatact ccccggaaaa ctggatgctg
    12421 cacgcaattc actcattgtt cagtggcatt actaccaggg gcgggttgaa gagatcctga
    12481 atggctggaa ctccccggaa gcacaattag cagaacaggc gctccgcagc ggtcacatag
    12541 aagcgttaat taacatatgg gaaaatgaca actactcacg ttatcgtccg gaaaagagtg
    12601 tctggaacct gtatttattg gcacagttgc cgcgtgagat ggctttgacc ttctggctgc
    12661 gtatcaatga gaaaaaacat ctgttcgcgg gtgaggacta ttttctcagt atcctcgggt
    12721 tggatgcgct accaggtctg atgttggctt tttcacatcg tccaaaagaa acatttccgt
    12781 taattttaaa ttttggcgca acagaactgg ccctgcccgt tgcccgcgtc tggcgacgtt
    12841 tcgcggtgca gcgtgatctg gctcgccagt ggattttaca ttggccggaa catacggcta
    12901 ctgcacttat ccctcttgtc ttcaccaaat ccagcgataa tagcgaagct gcattacttg
    12961 ccctgcgttt actttacgaa cagggacatg gtgaattgct gcaaaccgtg gcaaaccgct
    13021 ggcagcgtac agatgtatgg cctgccctgg agcatttact taaacagagt ccaatagaga
    13081 tttacccggc acgcattcca aaagcccctg atttctggca tccgcaaatg tggtccaggc
    13141 cgcgccttat cactaataat caaactgtta ccgatgacgc tctggaaatt atcggcgaaa
    13201 tgctgcgctt tacccagggg ggacgttttt atagcgggct ggaacaactg aaaacgttct
    13261 gccagccaca aacgctggca gcttttgcct gggatctctt cactgcgtgg caacaagctg
    13321 gtgcccccgc aaaagacaac tgggcatttc tggcgttaag tctctttggt gacgaaagca
    13381 cggcacggga tctgacgaca cagatcctcg cctggccaca agaaggcaaa tctgcccgtg
    13441 ctgtcagcgg cctgaacatc cttaccctga tgaataatga tatggcgctg atacagctgc
    13501 atcatatatc gcaacgggct aaatcccgcc ccttacgtga taacgcggcg gaatttcttc
    13561 aggtggtcgc agaaaatcgc gggctaagcc aggaagagct agcggacaga ttagtcccaa
    13621 ccctgggcct tgatgatccg caggcgttga gttttgattt tggtccccgg cattttaccg
    13681 ttcgcttcga tgaaaacctt aacccggtta tctttgatca gcaaaacgtt cgccagaaaa
    13741 gcgttccccg gttgcgcgcc gatgacgatc aactgaaagc gcccgaggca ctggcccgac
    13801 taaaagggct aaaaaaagat gccactcagg tgagcaaaaa cctgctcccg cgtcttgaaa
    13861 cggccctacg taccactcga cgctggtcgc tggcagattt tcattctctg tttgttaatc
    13921 atccctttac ccgtctggtt acccagcgat taatatgggg ggtttatccg gcaaatgaac
    13981 cgcgttgttt actcaacgcc tttcgtgtgg ccgcagaggg ggagttctgc aatgcgcaag
    14041 atgagccaat tgacctgcct gcggacgctc tgattggcat tgcccacctg ttagaaatga
    14101 cagtagaaat gcgcagtgaa tttgcacagc tttttgccga ttacgaaatt atgccgcctt
    14161 ttcgccagtt gtcgcggcgc acggtgctgc tcacacctga cgagtcaacc agtaacagcc
    14221 tgactcgctg ggaaggtaaa tccgctaccg ttgggcaact tatgggaatg cgatacaaag
    14281 gctgggagtc aggctatgag gacgcatttg tctatgacct gggtgagtac cggctggtcc
    14341 ttaagttttc acccggtttt aaccactaca atgttgatag caaagcgcta atgagcttcc
    14401 gttctcttcg agtgtaccgt gacaataaat ccgtcacttt tgccgaactt gatgtgtttg
    14461 atttgagtga ggcgttaagc gcacctgacg tcattttcca ttaaaacaca gcgggcagtg
    14521 atgcaactgc ccgttatcaa cacgacttac cagcggggaa agcgatgatc gtgcaaaaag
    14581 agctagttgc tatttacgat tatgaggtcc ctgtacctga agatccgttt tccttcagac
    14641 ttgagatcca taaatgctct gaattattta caggttccgt ctatcgactg gagcgattcc
    14701 ggctacatcc aacatttcat caacgtgatc gagaagatgc tgacccgcta ataaatgatg
    14761 cgttgattta tataagagat gagtgtattg atgagcggaa attacgaggt gaatcacctg
    14821 aaactgtaat agcaattttt aatcgtgaac tacagaatat attcaaccaa gaaatagaat
    14881 aatatactct agataattaa agtcgcagca catgcaactt gaagtatgac gagtatagct
    14941 ttcttaaagg gggaaacggt attccccctt agatgcgttt caatttgccc gttacttcaa
    15001 caaatcaatc aaccgtaaca gagccactaa taactccagt acggcaatga gcacggttaa
    15061 cttttgtgtc agcgtgtcca tccgctctcc ttattaagga gcgcaacacc agcaccttac
    15121 ccttacactg cctgtcgata ctggtttacg tatgttggta cgaaacgttg ctctccggat
    15181 cagggcaccg cctgcggcac cacccgtcca gcagccagga cttaaaattg gtccgatccg
    15241 gcacaacctc ccaacgggca ggattataaa tctcattcac tgaattgcaa tggagtgtgg
    15301 atatggttat aatttattcg ttggtacgaa atgttgcaca atcagcaagt tagcgtcact
    15361 gtagtaaata aaagaacctg cctcaccagc aggttttttt attttctgcg atctactttc
    15421 cagatatttt ttgccaatag cccaccataa atattcacat cccggtcaaa tggtgcgttg
    15481 aatataaaat cgatgaactt ataaataaac atgagttata tgatgaatta ataaaaacag
    15541 taatcatttt attaaagggg gaagcggttt tcccccttag atgcgtttca atttgcccgt
    15601 tacttcaaca aatcaatcaa ccgtaacaga gctactaata gctccagtac agcaatgagc
    15661 acggttaact tttgtgtcag cgtgtccatc cgctctcctt attaaggagc gcaacaccag
    15721 caccttaccc ttaaactgcc tgtcgatact ggtttacgta tgttggacca cgatgttgca
    15781 ctccggatca gggcaccgcc tgcagcacca cccgtccggt agccaggact tgaaataggt
    15841 tcgatccggc gcaacgtccc aacggtctgg attataaatc tcattatctt aattgtaacg
    15901 gggtccagcc gtggttataa ttcgttcgtt ggaacacgat gttgcacagg ctgtggtgtt
    15961 aggcctgaaa atagtaaata aaagaacctg cctcaccagc aggttttttt atttactgtg
    16021 atctgctttc cagatatttt tcgctcaaac aactaatgcg ccaaacattt attgcgcgta
    16081 aaatatcgtt tatttcatta atacatttca gggatgaata tatgtcaccg cagaataatc
    16141 atctgcagcg tccgcctgct gctgtgttat acgccgatga actggcaaaa ttaaaacaaa
    16201 atgatgccgc accttgcccg cccggttggc agttaagttt gcctgcggcc cgtgctttta
    16261 tccttggcga tagcgcgcaa aatattagcc gtaaagtggt gattagtccc tccgctgtcg
    16321 aacgtatgtt agtcactctt gccaccgggc gcggtttgat gttggtgggg gaacctggca
    16381 ccgcaaaatc tctcctgtct gaattactgg caaccgctat tagcggcgat gccgggttaa
    16441 ccatccaggg cggcgcatct actactgaag atcaaatcaa gtacggctgg aactacgccc
    16501 tgcttatcaa tcatggtcca tcaacggaag cgctggtccc tgccccgctt taccagggaa
    16561 tgcgcgacgg caaaatcgtc cgttttgaag agataacgcg tacaccgctg gaagtgcaag
    16621 actgtctgtt aggtatgctt tctgacaggg tgatgacggt gccggaactc actggtgaag
    16681 ccagtcagct ctatgcgcga gaaggcttta atattattgc cactgccaat acccgcgatc
    16741 gcggcgtcaa tgaaatgagt gctgcgctaa agcgtcgctt cgattttgaa actgtctttc
    16801 cgattatgga tttcgcccag gagctggagc ttgtcgccag tgcttcggcg cgtttgctgg
    16861 cccatagcgg tattccgcat aaagtgcctg acgcggtact ggagttactg gtccgcacct
    16921 tccgcgatct gcgtgccaac ggcgaaaaga aaacctcaat ggatacacta acagcgatta
    16981 tgtccaccgc tgaagccgtg aacgtggccc acgccgtggg cgtccgcgcc tggtttttag
    17041 cgaatcgcgc aggtgagcca gccgatctgg tggactgcat tgccggaacc atcgtcaaag
    17101 ataacgaaga agatcgcgct cgtctgcgcc gttacttcga acagcgtgtc gccacacata
    17161 aagaagctca ctggcaggct tattatcaag cccgccaccg cctgccgtga ggaaagatgc
    17221 atgagcgagc cgttaattgt cggcatccgg catcatagtc cggcctgcgc ccggctggtg
    17281 aaatcgttaa tcgaaagcca gcggccacga tacgtgttga ttgaaggccc ggctgatttt
    17341 aatgaccggg tagacgaact gtttttagcc caccagcttc cggtagctat ttacagttat
    17401 tgccagtatc aggacggtgc agcccccggg cgtggtgcct ggacgccatt tgctgaattt
    17461 tcgccggagt ggcaggcgct acaagccgct cgtcgtattc aggcacaaac ttacttcatc
    17521 gatttgcctt gctgggcaca gagtgaagaa gaggacgatt cgcctgatac gcaagaggaa
    17581 agccagacct tgctgctgcg tgccacccgc atggataaca gcgataccct gtgggatcac
    17641 ttgttcgaag atgaaagcca gcaaactgca ttaccctctg cactggcgca ctattttgcc
    17701 caactgcggg gcgatttcct cggcgatgca ctcaatcgtc agcgcgaagc ctttatggct
    17761 cgctggattg catgggcggt gcagcaaaat aatggcgacg tgttagtcgt ctgcggtggc
    17821 tggcacgctc cggcactggc aaaaatgtgg cgcgaatgcc cgcaggacat taacaagcca
    17881 gaattgccct cgctggcaga tgccgttaca ggttgttatc tcacacccta cagtgaaaag
    17941 cgccttgatg tgctggcagg atacctttca ggaatgcctg ccccggtctg gcaaagctgg
    18001 tgctggcagt ggggcttaca gcaggcggga gaacaactgc taaaaacggt tcttacccgt
    18061 ttgcgccaac accacttgcc tgcttcgacc gcggatatgg ctgccgctca tctgcatgcg
    18121 atggcgctgg cacagttgcg cggacataaa ctaccgttac gcactgactg gctggatgcc
    18181 atagcaggct cgctgattaa agaagccctg aacgcgccgt tgccgtggag ctatcgcgga
    18241 gttattcatc ccgacaccga tccgattctg ctaacgttga tagacacatt agcgggtgac
    18301 ggattcggta aacttgcccc ttctacgcca caaccgcctc tgccaaaaga tgtcacctgc
    18361 gaactggaac gtaccgcaat ctctcttccg gcggagctta ccttaaatcg ctttaacccc
    18421 aatggactag cgcaaagtca ggtgttacat cggctggcaa tactggagat cccagggatt
    18481 gtacgccagc agggaagcac actgacactt gcaggcaacg gtgaagaacg ctggaaatta
    18541 acccgcccgc ttagccagca tgcggcattg attgaggccg cctgctttgg tgccacactc
    18601 caggaagccg cacgccataa attagaagcc gatatgctgg acgcgggtgg aatcggcagt
    18661 atcaccacat gtcttagcca ggcggcgtta gcgggtctgg cgtccttcag tcaacaatta
    18721 ctggagcaac tcacattatt aatcgcccag gaaaatcaat ttgccgaaat ggggcaggcg
    18781 ctggaagtcc tttatgcctt atggcggtta gatgaaatca gcggtatgca aggcgcgcag
    18841 atattgcaga cgacgttatg tgcggctatc gatcgcacgc tgtggctgtg tgaatccgac
    18901 ggcaaaccgg atgaaaagga gtttcacgct cacctgcata gctggcaggc gctttgccat
    18961 atcctgcgcg atctacatag cggcgttaat ttacccggcg tttctctttc tgcggcggtg
    19021 gccttactgg aacgccgcag tcaggcaatt catgtcccgg cgctggatcg cggcgcggct
    19081 cttggcgcac tgatgcgtct ggaacatccc aacgccagtg ccgaagcggc gctgacaatg
    19141 ctggcgcagt tatccccggc acaatccggc gaggcgctgc acggtttgct ggcgctcgcc
    19201 cgtcatcaac tggcctgcca gccggcattt atcgccggtt tcagcagtca tttaaatcaa
    19261 ctgaatgacg acgattttat taacgccctg cccgatttac gcgcggcgat ggcctggcta
    19321 ccaccacgag aacgcgggac gctggcgcat caggtgcttg agcattatca actggcgcaa
    19381 cttcccgttt cggcgctgca aatgccgttg cattgtccac cacaagccat tgcacatcat
    19441 caacaactcg aacagcaggc actggcatcg ctgcaaaact ggggagtttt ccatgtctga
    19501 actgaacgat cttctgacca accgtgagct acaacgctgg cgattaattc ttggcgaagc
    19561 ggcagaaacg acgctttgtg ggctggatga caacgcccgg cagatagacc acgcgctgga
    19621 gtggctgtat gggcgcgatc ctgaacggct acagcgtggt gaacgctccg gtggattagg
    19681 tggctcaaat ctcaccaccc ctgagtggat caacagtatt cacacgctgt ttccgcaaca
    19741 ggtgattgag cggctggaaa gcgatgccgt actgcgctat ggcattgaag atgtggtgac
    19801 gaatctcgac gtgctggaac gtatgcagcc ttctgaaagc ctgctacgcg ccgttttgca
    19861 caccaaacat ctgatgaacc ccgaagtact ggctgccgcc cgccggatag tgcgccaggt
    19921 tgttgaagaa attatggctc gactggcaaa ggaagttcgt caggcttttt ctggtgtccg
    19981 cgatcgccgt cgccgctcat ctattccact ggcgcgaaac tttgatttca aaagtactct
    20041 acgcgccaac ctgcaacact ggcacccaca acacagcaag ttgtatatcg aatccccccg
    20101 ctttaacagc cgtattaagc gccacagtga acaatggcaa ctggtcttac tggttgatca
    20161 aagcggatcg atggtcgatt cggtgatcca ctctgcggtg atggcggcct gtttgtggca
    20221 gttacccggc attcgtaccc atctggtggc gtttgacaca agcgtcgtta atctcacggc
    20281 agacgttgcc gatccggtag agttattaat gaaagtacag ttgggcggcg ggaccagtat
    20341 cgccagtgcc gtggagtatg ggcggcaact tattgaacaa ccagcaaaaa gcgtcattat
    20401 cctcgtgagc gatttttacg aagggggttc atcatcattg ctgacgcatc aggtgaaaaa
    20461 gtgtgtccag agcggcatca aagtgctggg actggcagcg ctcgatagca cagcaacgcc
    20521 ttgctatgac cgcgatacgg cccaggcgct ggttaatgtc ggcgcacaaa tagccgccat
    20581 gacgccgggc gagctggcat catggcttgc ggagaatctt cagtcatgaa ttcactacgt
    20641 ccggaattat tagaactgac accgcaggca ctgacggcgt taagcaatgc cggttttgtt
    20701 aagcgcagcc ttaaggaact ggaaaatggc aacgtcccgg aaatcagcca tgagaacggc
    20761 gctttaatcg ccaccttcag tgacggtgtc cgtacccagc tggcgaacgg tcaggcactg
    20821 aaagaggctc agtgcagctg cggggccagc ggcatgtgcc gtcatcgcgt gatgctggtg
    20881 ttaagttatc aacgactttg tgctaccgcc cagcctacgg gaaaagaaga ggaatgggat
    20941 ccggcaatct ggctggaaga actggctacc cttcccgatg ccacccgcaa acgcgcacag
    21001 gcgctggtcg ctaaaggcat caccattgag ttgttctgta cgccgggcga aattccctct
    21061 gcccgcttac cgatgagcga tgtgcgtttt tattcccgta gcagcattcg tttcgcccgt
    21121 tgtgattgta ttgaaggcac actttgcgaa catgtcgtac tggcagtaca ggcctttgtc
    21181 caggcaaaag cgcagcaagc ggaattgact catttaatct ggcagatgcg cagcgaacac
    21241 gtcacatcat ctgacgaccc gtttgccagc gaagaaggca aaacgtgtcg tcaatatgtt
    21301 cagcaactaa gccaggcatt atggctgggc ggcatcagcc agccgcttat tcactacgag
    21361 gccgctttca gccgcgcgca gcaagcagcg gaacgctgca actggcgatg ggtgagtgaa
    21421 tcgctacggc aactgcgcgc cagcgttgat gccttccacg cccgcgccag ccactatcat
    21481 gccggagaat gcttacgtca gcttgcggca ttaaacagtc gattaaattg cgcacaagag
    21541 atggcccagc gcgacagtgt tggtgaagtt cctcctgtgc cgtggcgcac ggtcgttggc
    21601 tctggcattg ccggagaagc aaagcttgat catctgcggc tggtgtcttt aggtatgcgt
    21661 tgctggcagg atattgagca ttatggttta cgcatctggt ttaccgatcc cgacaccggc
    21721 agtattttgc acctttcgcg cagttggccg cgaagtgaac aggaaaactc accggcagct
    21781 acgcgtcggc tgtttagttt tcaggctggc gcactggcgg gtgggcaaat tgtttcacaa
    21841 gcagcaaaac gcagtgccga tggcgagctg ctgttagcta cccgcaaccg cttaagcagc
    21901 gttgtgccgc tgtcgcctga tgcctggcga atgttgagcg cgccgttacg ccagccgggc
    21961 attgtggctt tgcgggaata tttacgccag cgtcccccct cctgcatacg gcctcttaat
    22021 caggtcgata acttatttat tctgccggtc gctgagtgta tttcgctcgg ttgggatagc
    22081 agccgccaga cgctggatgc gcaggtaatc agcggcgaag gtgaagataa tctgctgacg
    22141 ttatcactgc cagcctcagc cagcgcgcct tatgccgttg aacgcatggc ggcgcttttg
    22201 caacaaacag acgaccccgt gtgtctggtt tctggctttg tcagttttgt tgatgggcaa
    22261 ttgacactgg aaccacaggt aatgatgaca aagactcgtg cctgggcgct ggacgcagaa
    22321 actgcgcctg tggtggcatc actaccttct gccggcgttc tgcctgtgcc gtctactgct
    22381 catcagttgc tgatgcgctg ccaggcatta cttattcaac tgctccataa cggctggcgc
    22441 tatcaggaac agagtgctat tggtcaggca gagttgctgg cgaatgacct caccgcggtg
    22501 ggtttttatc ggctggcaca tatgttggga caatttcgta atacagaaag cgaggcacgg
    22561 gtagaagcta tgaataacgg tgttttgctg tgcgaacaat tattccccat gcttcagcaa
    22621 caaggatgaa atagtgcttt ttactaagag ttctactcca gttccggact gctcacgcca
    22681 cggtattagg catatcctat atagcccctg gtgagagtca ccagttcctt gattaaataa
    22741 aatggagttt tacatgaagg ctttcaataa gctgttttcc cttgttgttg catctgttct
    22801 ggttttctct cttgctggct gcggtgacaa agaagaatcg aagaaattca gcgccaatct
    22861 gaacggcact gaaattgcca ttacctatgt ctacaaaggt gacaaggtgc ttaagcaatc
    22921 ttctgaaacc aaaattcaat ttgcctccat tggtgcaacc accaaagaag atgctgccaa
    22981 gacacttgag ccgttaagcg ccaaatacaa aaacatcgcg ggtgttgaag aaaaattaac
    23041 ctataccgat acctacgcgc aggaaaacgt gactatcgat atggaaaaag tggattttaa
    23101 agccctgcag ggtatttcag gaatcaacgt ttctgctgaa gatgccaaaa aaggtatcac
    23161 tatggcgcaa atggaactgg tgatgaaagc cgctggtttt aaagaagtga aataatctgt
    23221 cggcggccat gtttcgcatg gccgccatac ccgtcttagc ttttcttcac atgctggcgc
    23281 gctgccagtc cacgcagaaa ataacgtaaa aattgatcgc cgcattcgcg gaagttttta
    23341 tgatccggtg cgcgcatcat cgctgtaatt tccggcatcg aaacgcggaa ctgctgttcg
    23401 gtgaggatcg ccagaatgtc atcggttttc agcgaaaacg cgatgcgtaa ttttttcagc
    23461 acgatgttgt tattaatgcg acgttccggc tccagtgccg gagcagactc atccttgccg
    23521 cgtttttcat aaatcaggcc attgaggaat gacgacaaaa caatgtccgg acaacgctga
    23581 aaaccctctt cgtcttcttt acgtagccag acggcgatct gttccgcggt ggcttcgaca
    23641 ttacccagcg ccagtatacg caccaggtca ttattattgg ctttcaaaat gtagcgcacg
    23701 ctgcgcagaa tatcgttact tagcatgagg ccttcaggtg ttgatgaggc aaaaagccat
    23761 tttagcagtc ttttacaggc caatcgcctc ttttaagctt ttcagataac ggcggctgac
    23821 cggcacggtt aagccattac gcaaaatcaa ctcggcctgg ccgttatctt ccagacgaat
    23881 ctcctgtaaa tgcgcgaggt taaccagata ctgacgatgg cagcgcagta gtggtgtacg
    23941 actttccagg gtacgtaatg tcaattcggt aaagccctct ttcccttcgt ggctggtaac
    24001 gtagacaccg ctcatccgac tgctgacaaa tgccacatct ttcatttgca gcaaataaat
    24061 ccgactatgc ccagtacaag ggataaattt cagcgcctgt tgattttctg gtaacagcga
    24121 aacatcctgc ttgctgcgtt cctgccgcaa acgcgccagc gttttctcca gtcgcgcttc
    24181 atcaattggc ttcagcagat aatcaaaggc gtgttcttca aaggctttaa tggcgtattc
    24241 gtcaaacgcg gtgagaaaaa caatatacgg gcgatgttcc gggtcaagca tccccaccat
    24301 ttccagacca ctgatgcgcg gcatctggat atcgagaaac agcacatccg ggcgcagttt
    24361 atgcaccgcg ccgatccctt ccacggcgtt tgaacactca ccaacgattt caatatcgct
    24421 ctgctcctgc aaaaatacgc gcaggttctc ccgtgctaac ggttcatcat cgacaattaa
    24481 gactttaatc atgcctcgtc cctccatggt agtcgtaacg ttattcgggt gtaactatca
    24541 ggctcacagg cgacgcttat cccatagtca tcgccaaacc gttcacgtaa acgcttatcc
    24601 accagattca tccccagccc actggcattg gttaccggtt gatacaaacc ggcattgtct
    24661 tcgatctcca gcattaaatg ttgcccctca cgtcgggcgc tgattgccac tcgccctgta
    24721 tccagcagtt gtgatgtccc atgtttaatg gcgttttcca ctatcggttg cagggtaaac
    24781 gcgggcaatt gctgctggga taattcttgc ggaatagcaa tgttgacctg caaccgcgac
    24841 tggaagcgcg ccttttcaat ttgcagataa gcattcacat gttcaatttc gtcggcgaga
    24901 gtaacaaact ccgaaggccg ctttaagttt ttgcggaaaa aagtggaaag atactgcacc
    24961 agctggctgg cctgttcgct gtcgcggcgg atcaccgctt taatggtgtt aagcgcatta
    25021 aacaaaaaat gggggttcac ctgggcgtga agcagtttga tctctgactg ggtgagcatc
    25081 gctttttgcc gctcatattg cccggcaagg atctgcgccg aaagtagttg cgcaatcccc
    25141 tcacccaacg tgcggttgat tgaactgaat aaacggtttt tggcttcata caatttgatg
    25201 gtgcccatca cccgctgatt ttcaccacgt aacggaatta ccagcgtcga ccccagtttg
    25261 cattgcggat gcaaagagca acggtaaggt acttcgttgc catcagcgta gaccacttca
    25321 ccggtttcaa tcgcttttaa ggtataagtc gaagaaatcg gtttgccggg taaatggtgg
    25381 tcgtcaccaa ttccggtaaa ggccagcaat ttctctcgat cggtaatcgc gactgcacca
    25441 atatccagct cctgatacag cacctgtgcc actttcatgc tgttcacttc gttaaacccc
    25501 tggcgcaaaa tgccttccgt cgaggccgcc actttcagcg cagtggcaga aaaagctgaa
    25561 gtgtattttt caaacatcgc gcgtttatcg agcaatatac gcataaacag cgccgcgccg
    25621 acggtattgg tgaccatcat tggcgcagca atattactca ccagacgcac cgcatcttca
    25681 taaggtcggg cgatcgccag gatgatcaac atttgcacca tttcagcgac gaacgtgacg
    25741 gcaccggcgg taatggggtt aaagacttta tcagtgcgcc cgcgacggat caggatgctg
    25801 tgtaccaggc cgccgagcaa tccttcaacg atggtcgaga tcatgcaact tagcgcggtc
    25861 atgcccccca tcgaatatcg atgtaacccg ccggtcaggc caaccagccc accgacgacc
    25921 ggaccgccga gtaagccgcc cattaccgcg cctatcgcac gggtattggc aatagaatcg
    25981 tcaatgtgca acccaaacca ggtgcccatg atgcagaaga tggaaaagac gatgtagcag
    26041 agaaatttat gcggcagacg aaccgtgacc tgcattaacg gtatgaataa tggcgtttta
    26101 ctcattaacc acgcaatgac taaaaaaacg cacatctgct gaagcagcag caacaccaga
    26161 ttaaaatcgt acatacccgc aaaccacact tccctttaaa acgcgtaaca tacattgcct
    26221 gcgtttaact ttctttgaac tcttgcagaa aaatgagaat tcgtgagtac gatcactcaa
    26281 aatcgcctgg caaaaataaa atcaccctat aaatgcacaa aaaacgggca aaactacctg
    26341 gttcgcaaaa ctgcgtctaa agttaaaccg ggacctcgcg agcaagggtg agacgatggc
    26401 gctttacaca attggtgaag tggcgttgct ttgtgatatt aaccctgtca cgttacgcgc
    26461 gtggcagagg cgttacggat tgctgaaacc gcaacggaca gacggcggtc atcggctgtt
    26521 caacgatgcc gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca
    26581 ggtcagcaaa gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg
    26641 cgatcagcaa gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac
    26701 gtggatcaaa gagcgcggtc aggattaccc cgcccagacg ctcaccacac atctgtttat
    26761 tcctctgcgc cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct
    26821 cgacggcgta ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg
    26881 taaagatgcg ctggtggttg gctggaatat tcacgatacc acccgtctgt ggctggaggg
    26941 ctggattgcc agtcaacaag gctggcgcat tgatgtcctc gcccactcac tcaatcaact
    27001 acgccctgaa ctgttcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc
    27061 cgcccaacag cagcaactca ccagttggca agaacaaggc tatgatattt tcccactcgg
    27121 catttaatga ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag
    27181 tgcattatgc gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc
    27241 ggaattggtg gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct
    27301 gcaccagcct ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc
    27361 cctggatcac atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg
    27421 tgctggcccc taccgttgag gctatcgtcg ccgtaccaat gttgataatc accgaagttc
    27481 gcacgcccgc cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac
    27541 cacgactcat tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc
    27601 cggcaagtgt cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg
    27661 gttgcagacc aaacccgatc accggaacgg cgatcgccag caccgcgacg ggcggaaaag
    27721 tctgtccaac ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag
    27781 tgacagcaat tccggcacca gtaccaatga tcaccgcaaa caaactcgaa attcccacca
    27841 gccagaaatg agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca
    27901 gttgtgggaa caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca
    27961 caaacaaagc aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc
    28021 accagcagat cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc
    28081 agcacctcgc atccccgcgc gacaaacaga gagagcgcat cgcgtagcgt catctcttct
    28141 gccagtgcct caccttctgc tcgttcttcg cgacgcacgt aatccaccac actacgtaac
    28201 gaaagcaggc gcacacccag ttcactacgt ccaaaaaact ggcggacaaa atcattcgcc
    28261 ggacgagtca gcatcgtcag cggattcccc tgctgcacca cttcaccgtg atccatcaat
    28321 accagatgtt ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatagtg
    28381 cgccccagca aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg
    28441 tccagtgcgc caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc
    28501 gccactccca cacgttgctg ctgaccaccg gaaagctgat gtggataacg ctcacgtaaa
    28561 tttgactcca gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc
    28621 gaccattttt gtaattgcgg cacggtggca atgttttgtg ccacgctcca gtggggaaac
    28681 aggccaatag attgaatggc atagcccata cggcggcgca actccagtac tggcagcgag
    28741 cgaatttctt ctccggcaaa gcggatctct ccgctgtcat gttccaccag gcggttaatc
    28801 attttcaggg tggtggattt gccggaacca gatgtgccaa tcagcaccga aaaactccct
    28861 tcctgaaaat tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca
    28921 tggctaaatt caatcattac gtttcacctt caacagtgcg ataagtaaat cgaacagcgc
    28981 atcgatcagc accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc
    29041 gctgcttagc agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc
    29101 aatcaccgcc atgcctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg
    29161 taacgccagc ggtaactgaa catgcaggaa tcgctgcgcc ccgctcatcc ccatcgctct
    29221 ggcgctctcc agcacatcgc gcgggatctg gttcaagcct actaccacgc cgcgcaccag
    29281 cggcagcaag gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat
    29341 gccgaacttc cccagccacg gaaaggccgt aaccagtccg gcaagcggcg caatcagcag
    29401 gccaaagagc gccaccgaag gcacggtctg aatgacattg agcagagaaa aaattgcccc
    29461 ctgccgcgca gtggaaaaat agcaccagat gcccaacggc acaccaatca ctaacgcagg
    29521 cagcaccgca ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg
    29581 acggttggcg tattctttca ttagtgagag atcgttaagc gtgccggagt acagcaacca
    29641 cagcggaata atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat
    29701 tcggcggatg gcatcgctac aggccagcaa tgccagcgcc gcagccagcc agaaaccact
    29761 gccgaggctg gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc
    29821 tgctccccac accagcaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc
    29881 ttttttagcg ggaataaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca
    29941 aatcgtttgc ggccacagct gccagagatg tcgcccttca cccgagacta aacgattagg
    30001 cgcgtaactg ataaacggca gcgccgctgc gattgccgtc agcaacagca gcagcgccag
    30061 aacaggattg atacgtagat aagtcacggg aaattacttc gtccaccctt tttgtttcag
    30121 gtagtcggca gccacttttt tggcatccag tccttcaact gcaatgctgg cattcagttg
    30181 ctgcaatgtt ttttcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg
    30241 atactccttc aacaccgatt cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc
    30301 ttgcggatcg cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc
    30361 cattgcggca ttaacgccag aagtttgttg ggcagcggct ttaatcgtta ccgccgtgtc
    30421 gccgccagcc agtgacagta actgatcctg atcgagttta aagtcgtagg ctttttcaaa
    30481 cgcgggtaac gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc
    30541 gccctctttc agataacgac cgagatcggc aagcgaagtg agtttgtttt tctctgccac
    30601 atcctgacgc acggcgatgg tccaggtgtt attcgccggt gcaggcgtca gccagattaa
    30661 cttgttttgc tctgcatcga gttttttgac tttctcgtaa ccttgctgcg cgtttttcca
    30721 tgccgcatcg ttttcatctt taaagaagaa agcgccattg ccggtatatt ccggatagat
    30781 atccagttca ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt
    30841 attcaccgtc ggtacgccgt ggctttcgag tacttgcaaa atgatattgc cgagcagcgc
    30901 gccttcggta tcgatttttg aaccgacttt aacgggagaa gctgcctgta gcggcaggct
    30961 cacggctgcc aacataacca gtgaacctgc ccagaccttt gagagtggca tgatgctttc
    31021 ctcattcttt tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga
    31081 gttagcctga gcggacggat tcagttgcag aatcagataa atacttaaga ggcatattcg
    31141 gtccggcgtt tcctgccgga tgcggcgcga gcgccttatc cggcctacaa agggcgaaaa
    31201 ttcagcaagt taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag
    31261 cattacagca actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaagaca
    31321 ttgaacttgc caggctcggc gtcatatttc atctgttgat tccagaactt cagcgcctcg
    31381 atatcaatcg ggaagctaac ggtctgagtt tcgcccggtt tcagggtgat tttctcaaag
    31441 cctttcagct gtttcaccgg acgactcatg gaagccgtca catcctgcaa gtacatctgc
    31501 actaccgtgg ccccttcgcg tttaccggtg ttcgtcacct gcacgctggc cgtcactttg
    31561 ccgtcacgct tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag
    31621 ctcagaccat agccgaacgg ataaagcgcc ccgttagctt catcaaaata acgcgaagtg
    31681 tatttgttcg gcttgtcggc attatacgga cgaccggtgt tcagatggct gtagtacacc
    31741 gggatctgcc cgacagaacg cgggaaggac atcggcagct tgccggacgg gttgtaatcg
    31801 ccaaacaata catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga
    31861 atcgcatcag cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc
    31921 agcaccagcg gtttaccggt ggctttcagc gccgcaatca agtcacgttg gctttgcgga
    31981 atagtaatat cggtacggct ggaagcttcg tgcgccatcc cctgagcttc accgaccaca
    32041 gccaccacca catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc
    32101 gaacgcggat cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg
    32161 tcactggtaa cgttcgcccc tttggcatac agcactttac cgttttcacc gacggcattt
    32221 ttaatcccgg tcagcacggt cacggattga tcggcaacac cggctgcgga ccagctgccc
    32281 atcacgtcac gcttgctgtc tgccagcggg ccaaccaccg caatggttgc ggattttttc
    32341 agcggtaacg tttcgagacg gtttttcagc aacaccaggc tttcgcgcgc cacttcacgc
    32401 gcttctttgc ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcgga
    32461 ccgagatggc tgtatgggtc gttaaacaac cccatatcat atttaacgtt cagtacatgg
    32521 cgggcagcat cgtccagctc tgccatcgtc actttgccgg atttgatcaa cccaggcaga
    32581 tacttcgagt agtactcgtc gctcatgctc atgttgattc cggatttcag cgccacgcgc
    32641 accgcatctt ccgggtctgc cgccgtgcca tgtttaatca gttctttgat tgcaccgtga
    32701 tcggaaacgg tgatgccttt aaaaccccac tgatcgcgca gaacatcttt cagcagccag
    32761 gagtcggagg tggctggcgt gccgttgagt gagttcagcg ccaccattac cgcaccgctg
    32821 cctgcgtcca gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcgaactc
    32881 atatcgacgg tgttgtattc tttaccgcct tctaccgcgc cgtaagcggc aaagtgtttg
    32941 acgctggtca tcaccgagta gcgatctgcc gggcttttac cctgcatcgc ttccaccata
    33001 gttttaccca ttattgaagt gagatacgta tcttcgccaa aaccttcgga agcacgtccc
    33061 cagcgcggat cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct
    33121 gccgcttcat aagcagagac acgtccgacc gtttttactg catcgaggtt aaaagacgag
    33181 gccagaccga ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag
    33241 aaaagaggaa ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc
    33301 tgacgggtta cggtgttgaa aatcgctcca acctgaccgt ctttgatcat ctcgcggatc
    33361 gcctctttcg ggttatccgg gccgacgctg attaagcgca gctgaccaat tttctcatca
    33421 actgtcattt tcttaagcag ttcggtgacg aacgcatccc gcgcttcagg cgttaatgga
    33481 tggttaccga ataaatcatc cgccagtgct ggctgcaggg ccagactcac cgcgattcct
    33541 actgaacata gccatttcat atggattttt cttcctttgt tgcccgacgt ggcagcgaaa
    33601 atggtgcaaa aaccgtagtt tgccataagc atgatggaga gagaaaaaga atgctcagtt
    33661 tattgtctga attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt
    33721 ttgtgatatt ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata
    33781 ataaagcctt tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc
    33841 ccgcaaaaac ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg
    33901 tcgttttccc tggctcacta ctggaattgt ggcgggtgct gaaagcctgc gtcaccgccg
    33961 acaaaattat tctgatgcag gctgccaata caggcctgac cgaaggatcg acgccaaacg
    34021 gtaacgatta tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg
    34081 ttctcggcaa gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa
    34141 aagccctcaa accgctggga cgcgaaccgc actcggtgat tggatcatcg tgtataggcg
    34201 catcggtcat cggcggtatc tgtaataact ctggtggttc gctggtgcag cgtggcccgg
    34261 cgtataccga aatgtcgtta ttcgcgcgta taaatgaaga tggcaaactg acgctggtga
    34321 accatctggg gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgacg
    34381 atcgcatcaa agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg
    34441 tccaccgcgt tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatccggatc
    34501 ggttatttga atcttctggt tgcgccggga agctggccgt ctttgcagta cgtcttgata
    34561 ccttcgaagc ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc
    34621 tgaccgaaat ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat
    34681 atatgcaccg ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga
    34741 ttgataagtt aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg
    34801 cgatgctgga gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgcg atgcaaaaat
    34861 tcggtcacct gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg
    34921 agcatcatct gctgttaaaa atggcgggcg atggcgttgg tgaagccaaa tcgtggctgg
    34981 tggattattt caaacaggcc gagggcgatt tctttgtctg tacgccggag gaaggcagca
    35041 aagcgttttt acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt
    35101 ccgatgaagt cgaagacatt ctggcgctgg atatcgctct gcggcgtaac gacaccgagt
    35161 ggtatgagca tttaccgccg gagatcgaca gccagctggt gcacaagctc tattacggcc
    35221 attttatgtg ctatgtcttc catcaggatt acatcgttaa aaaaggcgtg gatgtgcatg
    35281 cgttgaaaga acagatgctg gagctgctac agcagcgcgg cgcgcaatac cctgccgaac
    35341 ataacgtcgg tcatttgtat aaagcaccgg agacgttgca gaagttttat cgcgagaacg
    35401 atccgaccaa cagtatgaat ccggggatcg gtaaaaccag taagcggaaa aactggcagg
    35461 aagtggagta aaaatgacgg atggcagagt atcgccatcc ctaattcact taatcgttct
    35521 gtgccgtctg ccccgccgcc gccatttggg cggctttttg ttttttatag ctcaacgctg
    35581 ctgccggaac aggcatcact ttaccggttt caatccaggt acgcaggcgg ctggcatcgg
    35641 caaaatgggt atatttgcca aacgcatcca tcactaccag cgccaccggt ttattattga
    35701 taaccgtacg catcaccaga caatggcccg ccgcattggt aaagccggtt ttggttagct
    35761 ggatattcca gttatcgcga tacaccagat gattagtatt gcggaacggc agcgtatacg
    35821 tcgggttaga gaaggttgcc atatcttccc gggtggtact taactgcccg atcaacggat
    35881 attgtttgct ggcaatgagc agtttggtta agtcacgggc ggttgaaacg ttatgcaccg
    35941 atagtccggt cggttcaaca aagcgcgtgt tgttcattcc gagcgatttc gctttcgcat
    36001 tcatcgcctt aataaaggct ttgtaaccac cgggataatg gtgcgcaagg cttgccgccg
    36061 cgcggttttc tgaagacatc aacgccagca acagcatatc tttacggctg atttcgctgt
    36121 tcagtcgtac gcgcgaatag acccctttca tctccggcgt ctggctgata tccactttta
    36181 gtttttcatc cagcggcagt cgcgcatcca gcacaaccat cgcggtcatt aatttgctga
    36241 tagacgcaat cggacgcacc agatccgggt ggttcgaata gatcactttg ttggtattca
    36301 gatcaacaat catcgcgcta ccggaggcaa tttccggttg tgaagcggtg gtagcggctg
    36361 ccgttttcgc aacggcctgc ggtgccaaag gcacagccag catcagggcc aggctaaata
    36421 aagaaactcg aaatttcggc atgatgagca ttcagatagt ggttcacgcg cacgggttgc
    36481 gcaccgccgg agtaaggatt tactgaggct agcgacgcca tcataacgag caaaaagtgc
    36541 gatcgtcaaa ggagaatcgt gaggaaatgc tgcattgctg acatttacgc cagcaatgca
    36601 acgtcaaaga actttctaga acaaacgata accgtagccc catagtataa cggtaagggc
    36661 gagcagcact tccagtacca gcacgccaat cgccagcgtc gaactggaga agctaaggcc
    36721 ttcctctttg ttgatattca ggaagctcgg aataccaagg taaagcaggt aaccggtgta
    36781 aaacagtgcc accgtgccga ccagcgcaca caaccagacc agcggataaa gcgccaccag
    36841 accgcttaaa aacagcggag ttgcaacgta gcccgcgaag accatgcagt gcgcaagtga
    36901 cggacgctgc gggtaattgc gcgccatcca ccagatgacc cgccccatca ccgcaacccc
    36961 agccagcata acgccgtaaa acaagacagc cagcgccagt ccggtaaacc aggataactt
    37021 caggatagtg ccatcgccaa aattccagcc aatctgtgta gtgccaatga aggcgcaaat
    37081 caccggaatc gccgccatca gcaaaacgtg gtgggtgtaa tggtgagaaa tcgtttcgtt
    37141 ttcgcgatta atcacctgca tttcacgatc gggatgggaa aacagtcccc agacatggct
    37201 cataccgccc ccttgttgtg agttcatgaa cctgacagtt caagtataag tcagcttgtg
    37261 attatttttt gttcgctcat gtaatttgca ccgtctgatg tacgtccttt ttcagcgtga
    37321 atgatttcca gggtgtatgc ttacaggcaa ccaaagggag acagactggc ctatggatct
    37381 caatacactc atctcacaat atggttatgc cgcgctggtg atcggtagcc tggcggaagg
    37441 tgaaaccgtg actttgctgg gaggcgttgc ggcgcatcag gggctattaa agttcccgct
    37501 ggtggtactt tctgtggcgc ttggcggcat gattggcgac caggtgcttt atctgtgcgg
    37561 gcggcggttt ggcggcaagc tgttacgccg tttctcgaaa catcaggata aaattgagcg
    37621 ggcgcagaaa attatccaac gccatccgta tctgtttgtc attggtacgc gctttatgta
    37681 tggctttcgg gtgattggcc cgacgctgat tggtgccagc cagctgccgc cgaaaatctt
    37741 tctgccgctg aatattctcg gcgcatttgc ctgggcgttg atttttacca ctatcggtta
    37801 cgctggtggt caggtgattg cgccgtggtt gcacaatctc gatcagcatt tgaagcactg
    37861 ggtctggttg attctggtcg tggttctggt ggtaggcgtg cgctggtggc tgaaacggcg
    37921 cgggaagaaa aagccggata atcaggcgta aaaccattgc cctggataag gcgttcgcgc
    37981 cgcatccgac atccggggca ccatgcctga tgcgacgctt gtgcgtctta tcatgcctac
    38041 caaaatttta cccattgaaa taaccacgca cctcactgcg ctattctggg ttgaactgcg
    38101 gattcgccaa cataaagccg ccatccacta tcaacgactg cccggtggtg taatttgcgc
    38161 cctctgagca cagccacgct accaggctgg caatctcatg cgttgcgcca aaacgccgca
    38221 gaggaatcga aggctccgcg tcgggcttca cgtcgctgtc atccatgcca ttcattggcg
    38281 tggcgatcgc cccaggcgcg actgcgttca ccaaaatctt atgtctgacc agctccagcg
    38341 ccattgcttt ggttaaccca ccgagcgcat gtttagcggc tgtgtaggcg ctggcatccg
    38401 gcagcggcgt atgttcatgt accgacgtaa tgttgatgat gcgaccgccc tgcccttgtt
    38461 tcaccatctg gcgggccgca atttgtgagc ataaaaatgc accatcgaca tcaacggtaa
    38521 aaatcttgcg ccactcatcg aaagccatat caagaaacgg cgctttggtc attgcgcccg
    38581 cattattcac cagcacatca atacgcccca gccgttgaat gagtttctcc aacgcctgcg
    38641 caccttctgg cagtttaccg agatccagct gcacaatctc cgcacgtacg ccgtggctaa
    38701 ctacctcacg cgcggtatct tttgcccctt cttcatctga gtgccaggta atgccaatat
    38761 caaacccttg ctgcgccagt aataacgcgc actctttgcc gatccccgaa tcggaggcgg
    38821 taataatcgc tacctgtgcc atcgagttct ccacttaacg ctgaataaac gttaagtata
    38881 gaagtcacat atcatcagcg tttgtaaccc ccgcccaacg caccagtgag ttgaatggag
    38941 gcatccagcc actgcccttg caataacagg ccattggccc gctcacgcag cgcggggatt
    39001 ctggcttcgc tgacgcggga accagcaatg atgcccgcgt taaagcgggc ctgcgcaaga
    39061 cctaccacac gcaaggcatc gcgctcaatt tgcgcctgat gctggttttt ctccgccagt
    39121 gtctgaacct gactggctgc ccgcgccacg tcattcaccg cttcaaccac cgctttgttg
    39181 tagctggcga tagacaagtt gctttcggct tttgcgatat cgagattggc gttaagacga
    39241 ccactatcga aaatgggtag cgtcaggcct gccgtaacgc ccatttgctg cgcggaatga
    39301 cggaacagct cgcttaagtg caacgcatcc tgttgcagga aggccatcag gttgatgtca
    39361 ggataaaatg ccgcttttgc cgcatcaatg gtgcttagcg atgactcaac gtaccagtgc
    39421 gccgcctgca aatctgcccg ccgggccagt aaggagtacc ccagttcatc aggaagctgg
    39481 cttgccactt tcggcaacgc gaccggttta agcttcaatg actttgtctg gtgatttgta
    39541 agtgcgctta accgtgcctc aataattttc attttccccg cgacatcgtt gagctgctgc
    39601 cgggttttgc tggcattaat atcggtttcc acaccttcaa ctgaagaagt aatcccgttc
    39661 tgatatagct ggcgatcggt cgcgataatg gtgttctgct ctttttcgat ttgctgcaag
    39721 accgtgttta acgccgcctg ggtttgccac tcccagtaca ggcgggctac gctgccagcc
    39781 agcaattggc gggtttgctc gcgttccgcc gcccgtgctt taaccgtacc caggcgggca
    39841 gtaacctccg cccgattctt tccccagata tcgagatgcc agcccgccgt taagccaaaa
    39901 gttccgttgg tgtaccacgg accggtcgta cctgcggccg gatcgttcag agcaaacggc
    39961 cccattagcc cttctgccga cattttttgc cgctccatat ccgcagaaaa gtcgatctgc
    40021 ggaccatcct gagtggcaat tgccttcgcc tgggcttcag ctagctgaat gcgctgttca
    40081 gccacctgca tatccggtgc gttctgtagt gcattgttaa ttaatgaagt gagttgatta
    40141 tcgtgatact ccagccacca ttggctgtct ggccaaccat ttttcagcgc cgtgggtaat
    40201 gcggtatcaa cttgtgcagc gggcgtttgc tggcttaacg cctggcgggt ttcatgcata
    40261 ggcgcacacc cggtcagcat cagtaacagc ggaaaacagg cgatggctgg ataaaaggaa
    40321 tcacgattca tgggggaata atcaggtaag aaaaggtgcg cggagattac cgtgtgttgc
    40381 gatatatttt ttagtttcgc gtggcaatac atcagtagca ataaaacgac atatccagaa
    40441 aaatatacac taagtgaatg atatcttccg atttatctta atcgtttatg gataacggca
    40501 aagggcttcg ttttttccta tacttattca gcactcacaa ataaaggaac gccaatgaaa
    40561 attatactct gggctgtatt gattattttc ctgattgggc tactggtggt gactggcgta
    40621 tttaagatga tattttaaaa atcagatatg acatataagt tgtcaggtca ggaactagat
    40681 attttgtgta cttgatttgc aaacagctcc ggtgttggtg gcgtcaaaat cgctgaagcg
    40741 ttccccgaag gccggggcag cccacatgga tgtgggctga gggcgcgtct tacaaggacg
    40801 tttcctcgcg cccgacccgg tagccgaagg ggataagctg a
//
LOCUS       DAZFKF010000037        40609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFKF010000037 DAZFKF010000000
VERSION     DAZFKF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.037"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            310..396
                     /gene="leuL"
                     /locus_tag="ACHKA1_002364"
     CDS             310..396
                     /gene="leuL"
                     /locus_tag="ACHKA1_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO5680004.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            489..2060
                     /gene="leuA"
                     /locus_tag="ACHKA1_002365"
     CDS             489..2060
                     /gene="leuA"
                     /locus_tag="ACHKA1_002365"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO5680005.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2060..3151
                     /gene="leuB"
                     /locus_tag="ACHKA1_002366"
     CDS             2060..3151
                     /gene="leuB"
                     /locus_tag="ACHKA1_002366"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO5680006.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYY
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASTIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3154..4554
                     /gene="leuC"
                     /locus_tag="ACHKA1_002367"
     CDS             3154..4554
                     /gene="leuC"
                     /locus_tag="ACHKA1_002367"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO5680007.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4565..5170
                     /gene="leuD"
                     /locus_tag="ACHKA1_002368"
     CDS             4565..5170
                     /gene="leuD"
                     /locus_tag="ACHKA1_002368"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO5680008.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(5278..5409)
                     /gene="sgrT"
                     /locus_tag="ACHKA1_002369"
     CDS             complement(5278..5409)
                     /gene="sgrT"
                     /locus_tag="ACHKA1_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO5680009.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            5498..7156
                     /gene="sgrR"
                     /locus_tag="ACHKA1_002370"
     CDS             5498..7156
                     /gene="sgrR"
                     /locus_tag="ACHKA1_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO5680010.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWISYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHARP
                     THCEKPAGLEHLTLTFYQDHIEHRVIARIMQQILASHQVTMEIKEISYDQWHKGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPLDWQADAARWRNGEINLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            complement(7385..7954)
                     /gene="espY2"
                     /locus_tag="ACHKA1_002371"
     CDS             complement(7385..7954)
                     /gene="espY2"
                     /locus_tag="ACHKA1_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HFO5680011.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMANLVKKTENMNIFE
                     ELWETLRNLFRSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDQLTHWLIK
                     FNELKEHARDPENMAAKASLSPKDTLCVSFFIGDEAIFTLKLQLKENTRTGCIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     regulatory      8196..8295
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8326..9309
                     /gene="thiB"
                     /locus_tag="ACHKA1_002372"
     CDS             8326..9309
                     /gene="thiB"
                     /locus_tag="ACHKA1_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO5680012.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9285..10895
                     /gene="thiP"
                     /locus_tag="ACHKA1_002373"
     CDS             9285..10895
                     /gene="thiP"
                     /locus_tag="ACHKA1_002373"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO5680013.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVIAVALAAFLALWSNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            10879..11577
                     /gene="thiQ"
                     /locus_tag="ACHKA1_002374"
     CDS             10879..11577
                     /gene="thiQ"
                     /locus_tag="ACHKA1_002374"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO5680014.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSIVVADGRIAWQGKTEELL
                     SGKASASALLGITG"
     gene            complement(11664..12428)
                     /gene="yabI"
                     /locus_tag="ACHKA1_002375"
     CDS             complement(11664..12428)
                     /gene="yabI"
                     /locus_tag="ACHKA1_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5680015.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12514..13392)
                     /gene="araC"
                     /locus_tag="ACHKA1_002376"
     CDS             complement(12514..13392)
                     /gene="araC"
                     /locus_tag="ACHKA1_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO5680016.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13731..15431
                     /gene="araB"
                     /locus_tag="ACHKA1_002377"
     CDS             13731..15431
                     /gene="araB"
                     /locus_tag="ACHKA1_002377"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO5680017.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHMP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15442..16944
                     /gene="araA"
                     /locus_tag="ACHKA1_002378"
     CDS             15442..16944
                     /gene="araA"
                     /locus_tag="ACHKA1_002378"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO5680018.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17113..17808
                     /gene="araD"
                     /locus_tag="ACHKA1_002379"
     CDS             17113..17808
                     /gene="araD"
                     /locus_tag="ACHKA1_002379"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO5680019.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            17883..20234
                     /gene="polB"
                     /locus_tag="ACHKA1_002380"
     CDS             17883..20234
                     /gene="polB"
                     /locus_tag="ACHKA1_002380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO5680020.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVFLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGNASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20398..23304
                     /gene="rapA"
                     /locus_tag="ACHKA1_002381"
     CDS             20398..23304
                     /gene="rapA"
                     /locus_tag="ACHKA1_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO5680021.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISVLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDTARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23316..23975
                     /gene="rluA"
                     /locus_tag="ACHKA1_002382"
     CDS             23316..23975
                     /gene="rluA"
                     /locus_tag="ACHKA1_002382"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO5680022.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24093..24908)
                     /gene="djlA"
                     /locus_tag="ACHKA1_002383"
     CDS             complement(24093..24908)
                     /gene="djlA"
                     /locus_tag="ACHKA1_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO5680023.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMERMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGDNWQQTQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25163..27517
                     /gene="lptD"
                     /locus_tag="ACHKA1_002384"
     CDS             25163..27517
                     /gene="lptD"
                     /locus_tag="ACHKA1_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO5680024.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27570..28856
                     /gene="surA"
                     /locus_tag="ACHKA1_002385"
     CDS             27570..28856
                     /gene="surA"
                     /locus_tag="ACHKA1_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO5680025.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            28856..29845
                     /gene="pdxA"
                     /locus_tag="ACHKA1_002386"
     CDS             28856..29845
                     /gene="pdxA"
                     /locus_tag="ACHKA1_002386"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO5680026.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAILGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLSGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            29842..30663
                     /gene="rsmA"
                     /locus_tag="ACHKA1_002387"
     CDS             29842..30663
                     /gene="rsmA"
                     /locus_tag="ACHKA1_002387"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO5680027.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30666..31043
                     /gene="apaG"
                     /locus_tag="ACHKA1_002388"
     CDS             30666..31043
                     /gene="apaG"
                     /locus_tag="ACHKA1_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO5680028.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31050..31898
                     /gene="apaH"
                     /locus_tag="ACHKA1_002389"
     CDS             31050..31898
                     /gene="apaH"
                     /locus_tag="ACHKA1_002389"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO5680029.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            complement(31895..32209)
                     /locus_tag="ACHKA1_002390"
     CDS             complement(31895..32209)
                     /locus_tag="ACHKA1_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFO5680030.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(32212..32445)
                     /locus_tag="ACHKA1_002391"
     CDS             complement(32212..32445)
                     /locus_tag="ACHKA1_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO5680031.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(32531..33010)
                     /gene="folA"
                     /locus_tag="ACHKA1_002392"
     CDS             complement(32531..33010)
                     /gene="folA"
                     /locus_tag="ACHKA1_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO5680032.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLAKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(33202..35064)
                     /gene="kefC"
                     /locus_tag="ACHKA1_002393"
     CDS             complement(33202..35064)
                     /gene="kefC"
                     /locus_tag="ACHKA1_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO5680033.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(35057..35587)
                     /gene="kefF"
                     /locus_tag="ACHKA1_002394"
     CDS             complement(35057..35587)
                     /gene="kefF"
                     /locus_tag="ACHKA1_002394"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO5680034.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLDGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGKSHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35698..37029)
                     /gene="yaaU"
                     /locus_tag="ACHKA1_002395"
     CDS             complement(35698..37029)
                     /gene="yaaU"
                     /locus_tag="ACHKA1_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5680035.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(37087..37374)
                     /gene="fixX"
                     /locus_tag="ACHKA1_002396"
     CDS             complement(37087..37374)
                     /gene="fixX"
                     /locus_tag="ACHKA1_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO5680036.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37371..38657)
                     /gene="fixC"
                     /locus_tag="ACHKA1_002397"
     CDS             complement(37371..38657)
                     /gene="fixC"
                     /locus_tag="ACHKA1_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5680037.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38708..39649)
                     /gene="fixB"
                     /locus_tag="ACHKA1_002398"
     CDS             complement(38708..39649)
                     /gene="fixB"
                     /locus_tag="ACHKA1_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO5680038.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGTDGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39664..40434)
                     /gene="fixA"
                     /locus_tag="ACHKA1_002399"
     CDS             complement(39664..40434)
                     /gene="fixA"
                     /locus_tag="ACHKA1_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO5680039.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9703 a        11102 c        10697 g         9107 t
ORIGIN      
        1 atatcaaaaa atccgcaaaa catataattc aatacaaatc atcaggatag gttttgcaac
       61 gcgtgcattt tgtccccttt ttcctcgtgg attagatgca aaaatttatt ctgaaatatg
      121 tcaaccgatg aaaagcgtcg gtagttaatc agaaattaat atcgcttgtt ttaaccacag
      181 cagcacaatt agctaatttt acggatgcag aactcatgct ggcgggacgt ttttattgcg
      241 tcagggttga catccgtttt tgtatccagt aactctaaaa gcatatcgca ttcatctgga
      301 gctgatttaa tgactcacat cgttcgcttt atcggtctac tactactaaa cgcatcttct
      361 ttgcgcggta gacgagtgag cggcatccag cattaagcca gcacgcagtc aaacaaaaaa
      421 cccgcgccat tgcgcgggtt tttttatgcc cgaagcgagg cgctctaaaa gagacaagga
      481 cccaaaccat gagccagcaa gtcattattt tcgataccac attgcgcgac ggtgaacagg
      541 cgttacaggc aagcttgagt gtgaaagaaa aactgcaaat tgcgctggcc cttgagcgta
      601 tgggtgttga cgtgatggaa gtcgggttcc ccgtctcttc gccgggtgat tttgaatcag
      661 tgcaaaccat cgctcgccag gttaaaaaca gccgcgtatg cgcgttagct cgctgcgtgg
      721 agaaagatat cgacgtggcg gccgaatccc tgaaagtcgc cgaagccttc cgtattcata
      781 cctttattgc cacttcgcca atgcacatcg ccaccaagct gcgcagcacg ctggacgagg
      841 tgatcgaacg cgctatctat atggtgaaac gcgcccgtaa ttacaccgat gatgttgaat
      901 tttcttgcga agatgccggg cgtacaccca ttgccgatct ggcgcgagtg gtcgaagcgg
      961 cgattaatgc cggtgccacc accatcaaca ttccggacac cgtgggctac accatgccgt
     1021 ttgagttcgc cggaatcatc agcggcctgt atgaacgcgt gcctaacatc gacaaagcca
     1081 ttatttccgt acatacccac gacgatttgg gcctggcggt cggcaactca ctggcggcgg
     1141 tacatgccgg tgcacgccag gtggaaggtg caatgaacgg gatcggcgag cgtgccggaa
     1201 actgttcact ggaagaagtc atcatggcga tcaaagttcg taaggatatt ctcaacgtcc
     1261 acaccgccat caatcaccag gagatatggc gcaccagcca gttagttagc cagatttgta
     1321 atatgccgat cccggcaaac aaagccattg ttggcagcgg cgcattcgca cactcctccg
     1381 gtatccacca ggatggtgtg ctgaaaaacc gcgaaaacta cgaaatcatg acaccagaat
     1441 ctattggtct gaaccaaatc cagctgaatc tgacctctcg ttcggggcgt gcggcggtga
     1501 aacatcgcat ggatgagatg gggtataaag aaagtgaata taatttagac aatttgtacg
     1561 acgccttcct gaagctggcg gacaaaaaag gtcaggtgtt tgattacgat ctggaggcgc
     1621 tggccttcat cggtaagcag caagaagagc cggagcattt ccgtctggat tacttcagcg
     1681 tgcagtctgg ctctaacgat atcgccaccg ccgccgtcaa actggcctgc ggcgaagaag
     1741 tcaaagcaga agccgccaac ggtaacggtc cggtcgatgc cgtctatcag gcgattaacc
     1801 gcatcactga ctataacgtc gaactggtga aatacagcct gaccgctaaa ggtcacggta
     1861 aagatgcgct gggtcaggtg gatattgtcg ccaactacaa cggtcgccgc ttccacggcg
     1921 tcggcctggc caccgatatt gtcgagtcct ccgccaaagc catggtgcat gtactgaaca
     1981 atatctggcg cgccgcagaa gtcgaaaaag agttgcaacg caaagctcaa cacaacgaaa
     2041 acaacaagga aaccgtgtga tgtcgaagaa ttaccatatt gccgtattgc cgggggacgg
     2101 tattggtccg gaagtgatga cccaggcgct gaaagtgctg gatgccgtgc gcaaccgctt
     2161 tgcgatgcgc atcaccacca gccattatta tgtaggcggc gcagccattg ataaccacgg
     2221 gcagccgctg ccgcctgcga cggttgaagg ttgtgagcaa gccgatgccg tgctgtttgg
     2281 ctcggtaggc ggtccgaagt gggaacattt accaccagac cagcaaccag aacgcggcgc
     2341 gctgctgcct ctgcgtaagc acttcaaatt attcagcaac ctgcgcccgg caaaactgta
     2401 tcaggggctg gaagcattct gtccgctgcg tgcagacatt gccgcaaacg gcttcgacat
     2461 cctgtgtgtg cgtgaactga ccggcggcat ctatttcggt cagccaaaag gccgcgaagg
     2521 tagcggacaa tatgaaaaag cgtttgatac cgaggtgtat caccgttttg agattgaacg
     2581 tatcgcccgc atcgcatttg aatctgctcg caagcgtcgc cacaaagtca cctcaatcga
     2641 caaagctaac gtgctgcaat cctctatttt atggcgtgaa atcgtcaacg agatcgccac
     2701 ggaatacccg gatgtcgaac tggcgcatat gtacatcgac aacgccacca tgcagctgat
     2761 taaagatcca tcacagtttg acgttctgct gtgctccaac ctgtttggcg acattctgtc
     2821 tgacgagtgc gcaatgatca ctggctcgat ggggatgttg ccttccgcca gcctgaacga
     2881 gcaaggtttt ggactgtatg aaccggcggg cggctcggca ccagatatcg caggcaaaaa
     2941 catcgccaac ccgattgcac aaatcctgtc gctggcactg ctgctgcgtt acagcctgga
     3001 tgccgatgat gcggcttcca ccattgaacg cgccattaac cgcgcattag aagaaggcat
     3061 tcgcaccggg gatttagccc gtggcgctgc cgccgttagt accgatgaaa tgggcgatat
     3121 cattgcccgc tatgtggcag aaggggtgta atcatggcta agacgttata cgaaaaattg
     3181 tttgacgctc acgtagtgta cgaagccgaa aacgaaaccc cgctgttata tatcgaccgc
     3241 cacctggtgc atgaagtgac ctcaccgcag gcgttcgatg gtctgcgcgc ccacggtcgc
     3301 ccggtacgtc agccggggaa aaccttcgcc accatggatc acaacgtttc aactcagacc
     3361 aaagacatta atgcctgcgg tgaaatggcg cgcatccaga tgcaggagct gatcaaaaac
     3421 tgcaaagaat ttggcgtcga gctgtatgac ctgaatcacc cgtatcaggg gatcgtccac
     3481 gtgatggggc cggaacaggg cgtgaccttg ccggggatga ccattgtctg cggcgactcg
     3541 cacaccgcca cccacggcgc gtttggcgca ctggcctttg gtatcggcac ttccgaagtt
     3601 gaacacgtac tggcaacgca aaccctgaaa cagggccgtg cgaagaccat gaaaattgaa
     3661 gtccagggca aagccgcgcc gggcattacc gcaaaagata tcgtgttggc aattatcggt
     3721 aaaactggca gcgcaggcgg caccgggcat gtggtggagt tttgcggcga agcaatccgt
     3781 gatttaagca tggaaggtcg gatgaccctg tgcaatatgg caatcgaaat gggcgcgaaa
     3841 gccggtctgg ttgcaccgga cgaaaccacc tttaactatg tcaaaggtcg tctgcatgcg
     3901 ccgaaaggca aagatttcga cgacgccgtt gcctactgga aaaccctgca aaccgacgaa
     3961 ggcgcaactt tcgataccgt tgtcactctg caagcagaag agatttcgcc gcaggtcacc
     4021 tggggcacca atccaggcca ggtgatttcc gtgaacgaca atattcccga tccagcttcg
     4081 tttgccgatc cggttgaacg cgcgtcggca gaaaaagcgc tggcctatat ggggctgaaa
     4141 ccgggtattc cgctgaccga agtggctatc gacaaagtgt ttatcggttc ctgtaccaac
     4201 tcgcgcattg aagatttacg cgcggcagcg gagatcgcca aaggccggaa agtcgcgcca
     4261 ggcgtgcagg cactggtggt tcccggctct ggcccggtaa aagcccaggc ggaagcggaa
     4321 ggtctggaca aaatctttat tgaagccggt tttgaatggc gcttgcctgg ctgctcaatg
     4381 tgtctggcga tgaacaacga ccgtctgaat ccgggcgaac gttgtgcctc caccagcaac
     4441 cgtaactttg aaggccgtca ggggcgcggc gggcgcacgc atctggtcag cccggcaatg
     4501 gctgccgccg cggctgtgac cggccatttc gccgacattc gcaacattaa ataaggagca
     4561 caccatggca gagaaattta tcaaacacac aggcctggtg gttccgctgg atgccgccaa
     4621 tgtcgatacc gatgcaatca tcccgaaaca gtttttgcag aaagtgaccc gtacgggttt
     4681 tggcgcgcat ctgtttaacg actggcgttt tctggatgaa aaaggccaac agccaaaccc
     4741 ggacttcgtg ctgaacttcc cgcagtatca gggggcttcc attttgctgg cgcgggaaaa
     4801 cttcggctgt ggctcctcgc gtgagcacgc gccctgggca ttgaccgact acggttttaa
     4861 ggtggtgatt gcgccgagtt ttgctgacat cttctatggc aatagcttta acaaccagct
     4921 gctgccggtg aaattaagcg acgcagaagt ggatgagctg tttgcgctgg tgaaagccaa
     4981 tccggggatt cattttgatg tagacctgga agcgcaagag gtgaaagcgg gtgagaaaac
     5041 ctatcgcttt accattgatg ccttccgccg ccactgcatg atgaacggtc tggacagcat
     5101 tgggcttacc ttgcagcacg acgacgccat tgcggcttat gaagcgaagc aacccgcgtt
     5161 tatgcgttag tttgtacacc gtccggtact caatcgccaa aaagaaaaaa gccagcagat
     5221 aatcactgct ggcgtggcga ttaacaccaa tactcagtca cacatgatgt ccgcaaatca
     5281 gtagtcagaa tgactcccct cccactgcat cagttcttca agcatggtta atcgttgtgg
     5341 gacgcttaac caacgcaacc agcacaactt cgctgtcgcg gtaaaatagt gctgataaaa
     5401 ctgacgcatg gggcacctcc ttgcttcatc gttaggaatt attgacttaa tatagggaaa
     5461 ataaaattgc tgtcttttgc gcaggagttc cccttttatg ccatctgctc gtctgcaaca
     5521 acagttcatc cgcctgtggc aatgctgcga gggtaaatcg caggacacaa cgctcaacga
     5581 actggcagcg ttattgagct gctcgcgtcg tcatatgcgc accctgctca acaccatgca
     5641 ggatcgcggc tggctgacgt gggaagcgga agtcgggcgt ggtaaacgct cgcgtctgac
     5701 attcctctat accgggctgg cgcttcagca acagcgggcg gaagacctgc tggagcagga
     5761 ccgtatcgac caactagtgc agttagttgg cgacaaagcg actgtgaggc aaatgctggt
     5821 ttctcatctg ggccgtagct tccgccaggg gcggcacatc ctgcgcgtgc tctactatcg
     5881 tccgttgcgt aatctgctac ctggcagcgc attgcgccgt tccgaaaccc atatcgcccg
     5941 gcaaatcttc agttcgctaa cgcgcataaa tgaggaaaat ggggaactgg aagcagacat
     6001 cgcccaccac tggcagcaaa tatcaccgct tcactggcgt ttctttttgc gtccaggagt
     6061 ccattttcat catggtcgtg aactggaaat ggacgacgtg atcgcctctt taaaacgaat
     6121 caatacgctg ccgctctatt cgcatattgc tgacattgtg tcgccgacgc cctggacgct
     6181 ggatatccac ctcacgcagc cggatcgctg gttgccgtta ctgctgggac aagttccggc
     6241 gatgatcctg ccgcgcgaat gggaaaccct cagtaacttt gccagccatc ccatcggcac
     6301 cggtccgtat gcggtgattc gcaacagcac caatcaactg aaaattcagg cattcgatga
     6361 cttcttcggt taccgggcat taatcgatga agttaatgtc tgggttctgc cggaaattgc
     6421 cgacgagcca gccggagggc tgatgctgaa aggcccacag ggcgaggaaa aagagattga
     6481 aagccgactg gaggaaggtt gttactattt actgttcgac agccgaaccc atcgcggggc
     6541 gaatcaacaa gtcagagact ggataagcta tgtgctttcg ccgaccaatc tggtctattt
     6601 cgccgaggaa cagtaccagc aactgtggtt tccggcctat gggctgctcc cccgctggca
     6661 ccacgcccgc ccaacacatt gcgaaaaacc tgccgggctg gaacatctca ccctgacgtt
     6721 ttatcaggat catattgaac atcgggtgat tgccaggatc atgcagcaga ttctggcgag
     6781 tcatcaggtc acgatggaaa tcaaagagat cagctacgat caatggcaca aaggtgagat
     6841 cgaaagcgac atatggctta acagcgccaa ctttacgctg ccgctggatt tttcgctgtt
     6901 cgcgcacttg tgcgaagtac ccctgctgca acactgtatt ccgctcgact ggcaagccga
     6961 cgcagcccgc tggcgcaatg gcgagataaa tctggcgaac tggtgccagc aactggtcgc
     7021 cagtaaagca atggtgccac ttatccacca ctggctgatc attcaggggc aacgcagtat
     7081 gcgcggcctg cgcatgaata ccctcggctg gttcgatttt aaatcagcgt ggtttgcgcc
     7141 gccggatcca gagtagcttg cgccttttgg cggtcatgag cgcatttttt atattcagac
     7201 ggaatgataa aatacattac ttttatattt tctctgtttt atgcatcgtc tgattagcgt
     7261 cgcaaataag attaaagaag gggcattcca gcccctcttt catattctga gttaatcaat
     7321 aacggcgcag tctagccttg ttctggccgg aaagatcatc tttttcaggt taacctcgcg
     7381 caggtcagtc ataccaacgg ctattgttcg tttcagcatt gctcaggtca acctcgagac
     7441 agtcaatacc acaaatgaca accccgttaa agtaagcatt actcagatca atgcacccgg
     7501 tcctggtgtt ctctttcagc tgcaacttca gagtaaatat cgcctcatcg ccaataaaaa
     7561 aactgacgca taacgtatct tttggcgaga gtgaggcctt cgcggccata ttttccggat
     7621 ctcttgcgtg ttctttgagt tcattaaatt ttatcagcca gtgagttaac tgatcacgcg
     7681 ccttcccgtc gacagccccg gtaaagtatt ttgagtagtc atcactattc tggaaataaa
     7741 atgcatcctt cagaatttgc cttgccgcag tctgggaatg tttatctgac cggaagagat
     7801 tgcgtaacgt ctcccatagc tcttcaaaga tattcatatt ctcggtcttt ttgaccaagt
     7861 tagccatcct gataatctga gaattagtaa ggcctggatc tttagtggta ttactagcag
     7921 ggatactaat ctgttctggg tttcttactt tcatgctgat agctccactc tgagtaaaaa
     7981 actcagaata agcgtcctga ataagaatac tgtcaaaaat cgctcattca cgcaataaac
     8041 ggttacgtta cctattttga ctgtagcgaa gggattcttt cggatcaacg ctctccagct
     8101 atgcaaatac gctcttgata gctgaacgaa tattggcttt tttaatacgc cttctctatg
     8161 gattgctggt tgataacaaa atcactacac taacgccgtt ctcaacgggg tgccacgcgg
     8221 acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc gaagggattt
     8281 gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt aaaaaaatgt
     8341 cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct gactgtttat
     8401 acctacgatt ccttcgccgc cgactggggg ccaggtccgg tggttaaaaa agcctttgaa
     8461 gccgactgta attgcgaact gaaactggtg gcgctggaag atggcgtttc gcttctcaac
     8521 cgtctacgga tggaaggcaa aaacagtaaa gccgatgtgg tgctggggct ggataacaac
     8581 ctgttagacg ccgccagtaa aaccggactg tttgccaaaa gcggtgtggc agcggatgcc
     8641 gttaacgttc ccggcggctg gaataatgac actttcgtac cgtttgatta tggctacttc
     8701 gccttcgttt atgacaagaa caaactgaaa aacccgccac aaagcctgaa agaactggtt
     8761 gagagcgatc aaaactggcg ggtgatttat caggatccgc gcaccagtac accggggctg
     8821 ggtctgttgc tatggatgca aaaagtctat ggcgatgacg ccccacaagc ctggcagaaa
     8881 ctggcgaaga aaaccgtcac cgtcaccaaa ggctggagcg aagcctacgg cctgttttta
     8941 aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca cattctcgaa
     9001 gagaagaaag ataactacgc cgccgcgaac ttcagcgaag gtcactatct gcaagtggaa
     9061 gtcgccgctc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt cctccagttt
     9121 atggtttctc cggctttcca gaatgcgatc ccaaccggca actggatgta tccggtggca
     9181 aacgtcacgc tgcctgccgg ttttgaaaaa ttgaccaaac ccgcaaccac gctggagttc
     9241 acgccagccg aagtggcggc acaacgtcag gcatggatta gcgaatggca acgcgccgtc
     9301 agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg gtgatagcgg
     9361 ttgcgctggc ggcgtttctc gccctgtggt cgaacgcgcc gcagggtgac tggtcggcag
     9421 tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag gcgtttctct
     9481 cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgcgctctat cgcaggcgct
     9541 ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc ccggtgctgg
     9601 ttgctgtttt cggcattctt agcgtctatg gtcgccaggg ctggctggca tcgctctgcc
     9661 aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggtatt ttgctggcgc
     9721 acgtattttt taatctaccg atggcgagcc gcttattact ccaggcactg gaaaacattc
     9781 ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat tttttccgct
     9841 tcgtcgaatg gccgtggtta cggagacaaa tcccgccggt tgctgcgctt atctttatgc
     9901 tctgtttcgc cagcttcgcc accgtactgt cgctgggcgg cggtccgcag gcgaccacta
     9961 tcgagctggc aatctatcag gcgctgagtt acgactacga tcctgcccgc gcggcgatgc
    10021 tggcgctgat ccagatggtg tgttgccttg ggctggtgct gttgagtcag cgactgagta
    10081 aggccattgc gcccggcacc acgctgctgc aaggctggcg cgacccggac gatcgtctgc
    10141 atagccgcat ttgcgacacg gtgttaattg tgctggcgct gctgctgttg cttccaccgt
    10201 tactggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg gcacaaccgg
    10261 tactgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggc gtattgtgcg
    10321 tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg cagaaaatgc
    10381 tggcgggtca ggcgctggag atgagtggca tgttgatcct cgccatgccg gggattgtgc
    10441 tggcaacggg cttcttttta ctgctcaaca acactatcgg cctgccacaa tctgctgacg
    10501 gcattgtgat tttcaccaat gcgttaatgg cgatccccta cgccctgaaa gtgctggaaa
    10561 acccgatgcg cgatatcacc gcccgctaca gcatgttatg tcagtcgctg gggattgaag
    10621 gctggtcacg cttaaaagtg gtggagctgc gcgccctgaa acgtccactg gcgcaggcgc
    10681 tggcctttgc ctgcgtgctg tcgattggtg attttggcgt ggtagcgttg ttcggtaacg
    10741 atgatttccg caccctgccg ttttatctct accagcaaat tggctcctat cgcagccagg
    10801 acggcgcggt caccgcgtta attctgctgc tactctgttt tctgctgttt accgtgattg
    10861 aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta ccaccatttg
    10921 ccgatgcgtt ttagcttaac agtagaacgc ggcgagcagg tggcgatcct cgggccaagc
    10981 ggcgcaggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc agccagcggt
    11041 tcgctgacta tcgatggcgt tgatcacacc acgacgccgc cgtcacgccg tccggtgtcg
    11101 atgctgtttc aggagaacaa cctgttcagc cacctgacgg tcgcgcagaa catcgggctg
    11161 gggctaaatc cgggattgaa actgaacgcg gcacagcagg agaaaatgca cgcgattgcc
    11221 cgtcagatgg ggattgataa tttaatggcg cggttacctg gcgagctttc cggcggtcag
    11281 cgacagcgag tggcgttagc gcgttgtctg gtacgcgaac agccgatttt attgctcgat
    11341 gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgttgacgct ggtgagcacg
    11401 agctgccagc agcaaaaaat gacgttattg atggtgtcgc acagtgtgga agatgcggca
    11461 aggattgcaa cacgctcgat agtggtcgcc gacgggcgca tcgcctggca gggtaagacc
    11521 gaagaattgt tgagcggtaa ggcgagtgct tcggcgctgt tgggaattac aggttaatat
    11581 gcactgtcgg atgcggcgtg aacgccttat ccgacctacg ggtgcgtttt gtccgcatca
    11641 agcgccccac acaagactat gccctaaact ccaaccactt tacgcaaaat atcgatatac
    11701 accggcatta acggatggcg aattaacacc accagcgcca ccacgccgat ggcagaaatc
    11761 aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg gtcagtcgct
    11821 ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa taccgccgtc
    11881 gccagcagca accatttaaa ctcaccgctc tgcattccgg ctggaatatc gatcgccgcg
    11941 cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc gataatattc
    12001 ggcgtaataa atttagccac cggcagatcc agcattcccg ccaccattgg caccagcgga
    12061 cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg atgcaacgca
    12121 tgttcagttt tatcgagtaa tgctttgttc ttcttcagaa atgaccagcg atgcaatggc
    12181 tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcagaca accgacgatc
    12241 cctgccagcc aggcgtgcca gaaacttaac tcgccgctgc caatcagcgc tcccagcccc
    12301 gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc cagaaaggcc
    12361 accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg ttccagcaat
    12421 gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc tatgctactc
    12481 cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg ctacatcatt
    12541 cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc attttttaaa
    12601 tactcgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg tggcgatagg
    12661 catccgggtg gtgctcaaaa gcagcttcgc ctggctgatg cgttggtcct cgcgccagct
    12721 taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg gcgacaagca
    12781 aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat cgctgatgta
    12841 ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt taatcgcttc
    12901 catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg aatagcgccc
    12961 ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc
    13021 ttcatccggg cgaaagaaac ccgtattggc aaatattgac ggccagttaa gccattcatg
    13081 ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag cctccggatg
    13141 acggccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg gtcggcagac
    13201 aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat tgagaatata
    13261 acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg
    13321 cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg gatcattttg
    13381 cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt gtccatattg
    13441 catcagatat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc aaccggtaac
    13501 cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa
    13561 caaaagtgtc tataatcacg gcagaaaagt ccacattgat aatttgcacg gcgtcacact
    13621 ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac gctttttttc
    13681 gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg atggcgattg
    13741 caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggac tgcgccagcg
    13801 gtgaagagat cgccaccagc gtagagtggt atccccgttg gcaaaaaggg caattttgtg
    13861 atgccccgaa taaccagttc cgtcatcatc cgcgtgacta cattgagtca atggaagcgg
    13921 cactgaaaac cgtgcttgca gagcttagcg tcgaacagcg cgcagctgtg gtcgggattg
    13981 gcgttgacag taccggctcg acgcccgcac cgattgatgc cgacggtaac gtgctggcgc
    14041 tgcgcccaga gtttgccgaa aacccgaacg cgatgttcgt attgtggaaa gaccacactg
    14101 cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac gttgactact
    14161 cccgctacat tggcggtatt tattccagcg aatggttctg ggcaaaaatc ctgcatgtga
    14221 ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg tgcgactggg
    14281 tgccagctct gctttccggt accacgcgcc cgcaggatat tcgtcgcgga cgttgcagcg
    14341 ccgggcataa gtcgctatgg cacgaaagct ggggcggcct gccgccagcc agtttctttg
    14401 acgaactgga cccgatcctc aatcgccatt tgccttcccc gctgttcact gatacctgga
    14461 ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc ggcctgcctg
    14521 aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt ggtgcaggcg
    14581 cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt ctgattgccg
    14641 acaaacagag cgttggcgag cgggcagtga aaggtatttg cggtcaggtt gatggcagcg
    14701 tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat atctacgcct
    14761 ggttcggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat ccggaactaa
    14821 aagagcaaat taacgccagc cagaaacaac tgcttccggc gctgaccgaa gcatgggcca
    14881 aaaatccgtc tctggatcac atgccggtgg tgctcgactg gtttaacggc cgccgcacac
    14941 cgaacgctaa ccaacgcctg aaaggggtga ttaccgacct taacctcgct accgatgctc
    15001 cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca atcatggagt
    15061 gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc atcgcgcgga
    15121 aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg caaattgttg
    15181 cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc gcgaaagtgc
    15241 acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa accctgcaac
    15301 cgtgcagcga gcaggcacaa cgctttgaac agctttatcg ccgctatcag caatgggcga
    15361 tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc caggccgttc
    15421 cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg gtttgtaatt
    15481 ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca tgccgagcac
    15541 gttgttaatg cgctaaatac ggaagcgaaa ctgccctgca aactggtgtt gaaaccgctg
    15601 ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga cgatcgttgc
    15661 gctggtctgg tggtatggct gcacaccttc tccccggcca aaatgtggat caacggcctg
    15721 accatgctca acaaaccgtt gctgcaattc cacacccagt tcaacgcggc gctgccgtgg
    15781 gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg tcgcgagttc
    15841 ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca ctggcaggat
    15901 aaacaagccc atgagcgtat cggctcctgg atgcgtcagg cggtctctaa acaggatacc
    15961 cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtggcggt caccgatggc
    16021 gataaagttg ccgcacaaat caagttcggt ttctccgtca atacctgggc ggttggcgat
    16081 ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt cgatgagtac
    16141 gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg acagaacgtg
    16201 ctggaagcgg cgcgtattga gctggggatg aaacgtttcc tggaacaagg tggcttccac
    16261 gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg tctggccgta
    16321 cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa aaccgccgcc
    16381 ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc ctttatggag
    16441 gactacacct atcacttcga gaaaggcaat gacctggtgc tcggctccca catgctggaa
    16501 gtctgcccgt cgatcgccgc agaagagaaa ccgatcctcg acgttcagca tctcggtatt
    16561 ggtggtaagg acgatcctgc ccgcctgatc ttcaataccc aaaccggccc agcgattgtc
    16621 gccagcctga ttgatctcgg cgatcgttac cgtctactgg ttaactgcat cgacacggtg
    16681 aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa agcgcaaccg
    16741 gatctgccaa ctgcttccga agcgtggatc ctcgcaggtg gcgcgcacca taccgtcttc
    16801 agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga cattgaaatc
    16861 acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcgctgcg ctggaacgaa
    16921 gtgtattacg gatttcgtcg ctaagtagcc gcatcaggtg tgtaacgcct gatgcgacgc
    16981 tgacgcgtct tatcaggcct acacgctgtg attttgtagg ccggataagc aaagcgcatc
    17041 cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg aaacgacctc
    17101 tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa tttggcgctg
    17161 ccaaaacata acctggtcac gctcacctgg ggcaacgtca gcgccgttga tcgcgagcgc
    17221 ggcgtctttg tgatcaaacc ttccggcgtc gattacagca tcatgaccgc cgacgatatg
    17281 gtcgtggtca gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc ctcctccgac
    17341 acgccaactc accggctgct ctatcaggca ttcccgtcca ttggcggcat tgtgcacaca
    17401 cactcgcgcc acgccaccat ctgggcgcag gcgggtcagt cgattccagc aactggtacc
    17461 acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac cgacgcagaa
    17521 atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt cgaaaaacag
    17581 ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt ctcacggccc atttgcatgg
    17641 ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt cgcttatatg
    17701 gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac gctgctggat
    17761 aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg actgtataaa
    17821 accacagcca atcaaatgaa accaggctat aatcaagcct ggttttttga tggattttca
    17881 gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggataccccg caaggaacag
    17941 aggtctcctt ctggctggcg acggacaacg ggccgttgca ggttttcctt gcgccgcaag
    18001 agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt ttgcggggtg
    18061 aacaaggctt tcgcctgacg ccgctggcgt taaaggattt tcaccgccag ccggtgtatg
    18121 gcctttactg tcgcgcccat cgccagttga tgaattacga aaagcgcctg cgtgaaggtg
    18181 gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg gagcggttta
    18241 tcacctcgcc ggtgtgggtc gagggtgata tgcacaatgg cgctatcgtt aatgcccgtc
    18301 tgaaaccgca tcccgactat cgcccgccgc tcaagtgggt gtctattgat attgaaacca
    18361 cccgccacgg tgagctgtat tgcatcggcc tggagggctg cgggcagcgc atcgtttata
    18421 tgctggggcc ggagaatggc aacgcttccg cgctcgattt cgaactggaa tacgtcgcca
    18481 gtcgcccgca gctactggaa aaacttaacg cctggtttgc caactacgat cctgatgtga
    18541 tcatcggttg gaacgtggtg caattcgatc tgcgaatgct gcaaaaacat gccgagcgtt
    18601 atcgtattcc gctgcggctg gggcgcgata acagcgagct ggagtggcgc gagcacggct
    18661 ttaaaaacgg cgtctttttt gcccaggcta aagggcggct aattatcgac ggtatcgagg
    18721 cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc gctcaggagc
    18781 tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa attgaccgcc
    18841 gtttcgccga agataaacct gcgctggcga cttataacct gaaagattgc gagctggtga
    18901 cgcagatttt ccataaaact gaaatcatgc cgtttttgct cgaacgagca acggtgaacg
    18961 gcctgccggt agaccgccac gggggatcgg tggcggcgtt tggtcatctc tattttcccc
    19021 ggatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg cacgccagcc
    19081 ctggcggcta cgtgatggat tcgcgacctg ggctttatga ttcggtgctg gtgctggact
    19141 acaaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc gggctggtgg
    19201 aaggcatggc gcagcctgat ccagagcaca gcaccgaagg ctttctcgat gcctggttct
    19261 cgcgagaaaa acattgcctg ccggagattg tgactaacat ctggcacggg cgcgatgaag
    19321 ccaaacgtca gggtaataag ccgctttcgc aggcgctgaa gatcattatg aatgcctttt
    19381 atggcgtgct cggcaccacc gcctgccgct tcttcgatcc gcggctggca tcgtcgatca
    19441 ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcgtt gattgaagcg cagggctacg
    19501 acgtgatcta cggcgatact gactcaacgt ttgtctggct gaaaggcgca cattcggaag
    19561 aagaagcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg tgggcggaaa
    19621 cgctgcaaaa acaacagctg accagcgcac tggaactgga gtatgaaacc catttctgcc
    19681 gttttctgat gccaaccatt cgcggagccg ataccggcag taaaaagcgt tatgccgggc
    19741 tgattcagga gggcgacaaa cagcggatgg tgtttaaagg gctggaaacc gtgcgtacgg
    19801 actggacgcc actggcccag cagtttcagc aggagcttta cctgcgtatc ttccgcaacg
    19861 agccatatca ggagtacgtc cgcgaaacca tcgacaaact gatggcgggt gaactggatg
    19921 cgcggctggt ttaccgtaaa cgccttcgcc gtccgctgag cgaatatcag cgtaatgttc
    19981 cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgc ggtcgcccct
    20041 tgcaatacca gaaccgcggc accattaagt acgtatggac caccaacggc ccggagccgc
    20101 tggactacca acgttcacca ctggattacg aacactatct gacccgccag ctacaacccg
    20161 tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg accgggcaac
    20221 ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg tgacgaatgc
    20281 tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca ccactacctc
    20341 ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg aacacatatg
    20401 ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg acttggaacc
    20461 gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg tgaaaaccgt
    20521 ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg tgataccatt
    20581 accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa tggcttgctg
    20641 acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg tgaagttttc
    20701 cttgatagca aactggtgtt cagcaaaccg caggaccgcc tgtttgccgg gcagattgac
    20761 cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga acagttccgt
    20821 atgccgtaca gcggcttgcg cggtcagcgt accagcctga tcccgcacca gctcaacatc
    20881 gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga agtgggttta
    20941 gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg cgctgctgaa
    21001 cgtgtgctga ttattgtccc ggaaacctta cagcatcagt ggctggtaga aatgctgcgc
    21061 cgtttcaacc tgcgttttgc gctgtttgat gatgagcgtt atgccgaagc tcagcacgat
    21121 gcttacaacc cgttcgatac cgaacaactg gtgatttgct cgctggattt tgcccgtcgt
    21181 agcaaacagc ggctggaaca tctctgtgaa gccgaatggg acctgctggt ggtcgatgaa
    21241 gcccatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc cattgaacaa
    21301 ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca gctggggatg
    21361 gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga ttttgcgcag
    21421 ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat gctgctggca
    21481 ggtaataaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg cgagcaggat
    21541 atcgagccgc tgttgcaagc agcaaacagc gacagcgaag atgcccagag cgcccgtcag
    21601 gaactggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt ccgtaacacc
    21661 cgtaacggtg tgaaaggctt cccgaaacgc gagttgcaca ccattaagct gccgctaccg
    21721 acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa aagtgcggaa
    21781 gatcgtgctc gagatatgct ctacccggag cgtatttatc aggaatttga aggtgataac
    21841 gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct gaccagccat
    21901 cgctctcaga aagtgctggt gatctgcgct aaagctgcca ctgcgctgca actggagcaa
    21961 gtactgcgcg aacgtgaagg tatccgcgct gctgtgttcc acgaaggcat gtcgattatt
    22021 gagcgtgacc gcgctgccgc ctggtttgct gaagaagaca ccggcgcaca ggtactgctg
    22081 tgctcggaaa tcggttctga aggacgtaac ttccagttcg ccagccacat ggtgatgttt
    22141 gacctgccat tcaacccgga tctgctggag cagcgtattg gtcgtctgga tcgtatcggt
    22201 caggcgcacg atattcagat ccatgtgcct tatctggaaa aaactgctca gtcggtgctg
    22261 gtgcgctggt atcacgaagg tctggatgca ttcgagcaca cctgcccgac cggacgcact
    22321 atttacgata gcgtctacaa cgatctgatt aactatctgg cttcaccgga tcaaaccgaa
    22381 ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa agcacaactg
    22441 gaacaggggc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa agcccaggca
    22501 ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt cgccatgaac
    22561 ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat cgtgctgacc
    22621 ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg catcaccatc
    22681 acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac ctgggagcat
    22741 ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag cagcacgatt
    22801 tcagtgttga aaaacaaagc gttaccggta ggtacgctgt tggtggaact gatttacgtg
    22861 gtcgaagccc aggcgccgaa gcagttgcag ctcaaccgct tcctgccacc gacgccggta
    22921 cgtatgctgc tggataaaaa tggcaacaac ctggcggccc aggtggagtt tgaaaccttt
    22981 aaccgccagc ttaacgcggt caaccgtcac accggcagca aactggttaa tgccgtgcag
    23041 caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc ggcccgtgca
    23101 ttgattgata ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct gtctcgtctg
    23161 gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc cattgaaagc
    23221 aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga tgccctgcgt
    23281 ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac tacaatccac
    23341 cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg gtcaacaagc
    23401 cgagcggttt gttgtctgtg ccgggtcgtc tggaagagca caaagacagc gtgatgacgc
    23461 gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg gctaccagcg
    23521 gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc cagttccgcg
    23581 agcgcgagcc gaaaaagcag tatgtggccc gcgtctgggg gcatccatcc cctgcggaag
    23641 gtctggtaga tctgccgctg atttgcgact ggccaaaccg cccgaaacag aaagtctgtt
    23701 acgaaacagg taaacctgcg cagacggaat atgaagtggt ggagtatgcg gcggataaca
    23761 cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcaattgcgt gtgcatatgc
    23821 tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccggaa gcgagagcga
    23881 tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg gcgtatggca
    23941 atagtatgac gtttaaagcg ccagcggatt tttaatagtc acactggcga agttcggttg
    24001 gccggagaag gcgttcacgc cgcatccggc aattttgcgc cgattgcctg atgcgacgcg
    24061 ggccgcgtct tatcaggcct acagtttgaa acttatttaa accctttctg ctgcttaatc
    24121 aattcatagg cctgctgaat ttcctgcgct ttttgcttcg ccatctccat catctcaggc
    24181 ggcaaacctt tcgccaccaa cttatcggga tggtgttcac tcatcagctt acggtaggca
    24241 cgtttgatgg tggtcgcatc atccgtcggc ttcacgccca gcacattaca ggcatcttcc
    24301 agcgtcgggc cacgctgggt ttgttgccag ttatcaccgc cagattgctg ctgataaccg
    24361 ccgccaaact gtgcaccgcc ctgcatcatg cgcaaaaact ggtcaaactg ggcgcgggag
    24421 atccctaatt cttcagcaat gacatacagc accgcccgtt cattcgggtg cagtgaacca
    24481 tcagcaaacg ccgcctgaat ctggatctcc agaaacatac gaattaagtc aaaacgacca
    24541 aagcagacac tgcgaaactg gcgcatcttt tcgcgcagcg ggtaattgtc tgattttccc
    24601 acccggaacg cgttttgcgc cgcagtacgg gaagcgccat gaaggttcat tcgctccatc
    24661 aactggctgg cgatatgaat atcagcctcc gtgacgcgac ctttggattt ggttaaatgc
    24721 cccatcactt caaaagtggt ggcaaaaaac agcgcctgac gctcacgctg gttggcaaac
    24781 cacgccattt tacggctacg ggctttatca aacatatggc caattaacag gcctaacact
    24841 acgccccaaa agccgccgcc catcagtaag gccacggcca cgccaatgat ttttccccaa
    24901 tactgcatat attccccaaa tcgacacacg gatatcaggg ctatctccca caatataaag
    24961 gtgcttttac cgttttccgg ctgcggtcaa ttgtgggaca tcgcctataa tttgcattat
    25021 catacctgtc attcaatgcc gtgcctaaca ccactgacgc tattcggaca ggattaacac
    25081 tagcgtagag atgacgagta cgttagtctc tgaccgtttg tcacgcgcaa cgttaccgat
    25141 gatggaacaa taaaatcaac gtatgaaaaa acgtatcccc actctcctgg ccaccatgat
    25201 tgccaccgcc ctttatagtc aacagggact ggcagccgac ctcgcctcac agtgcatgtt
    25261 gggcgtgcca agctatgacc gtcctctggt acagggcgat accaatgact tacccgtgac
    25321 tatcaatgct gaccacgcga aaggggacta cccggatgac gccgtgttta ctggcagcgt
    25381 ggatatcatg cagggtaaca gccgtctgca ggccgacgaa gtgcagctcc atcaaaaaga
    25441 ggcaccagga caaccggagc cggtacgtac cgttgatgcg ctcggtaatg tccattacga
    25501 cgataaccag gtgatcctca aagggccgaa aggctgggcg aatctgaaca ccaaagatac
    25561 caacgtctgg gagggtgatt accagatggt gggtcgccag ggccgcggta aagcggacct
    25621 gatgaaacaa cgtggcgaaa accgctatac cattctggat aacggtagct ttacctcctg
    25681 tctgccgggt tctgacacct ggagcgtggt aggtagcgaa attattcatg accgcgaaga
    25741 acaagtggcc gaaatctgga acgcccgttt taaagttggc ccggtaccaa tcttttatag
    25801 cccctatttg cagttgccgg tgggtgacaa acgtcgctct ggtttcttga tcccgaacgc
    25861 caagtacacc accaccaact actttgagtt ctacctgcca tattactgga acatcgcgcc
    25921 aaatatggat gccaccatca cgccgcatta tatgcatcgt cgtggcaaca tcatgtggga
    25981 gaacgaattc cgctacctct cccaggcggg cgctggcttg atggaactgg actatctgcc
    26041 ttcagataaa gtctatgaag atgaacaccc gaacgatgac agttcacgtc gttggttatt
    26101 ctactggaac cactccgggg tcatggatca ggtgtggcgt ttcaacgtcg actacaccaa
    26161 ggtcagcgat cctagctact tcaatgattt cgataacaag tacggttcca gtactgacgg
    26221 ctacgcaacg caaaaattca gcgttggcta tgcggtgcaa aacttcaatg ccaccgtttc
    26281 aaccaagcag ttccaggtct tcagcgaaca gaacaccagt agctactcgg cagagccgca
    26341 gttagacgtt aactactacc agaatgatgt tggtccgttt gatacgcgta tttacggcca
    26401 ggcagtgcac tttgttaaca ccagagacga catgcctgaa gcaacccgtg ttcacctgga
    26461 accgaccatc aatttgccgc tctctaataa ctggggcagt atcaataccg aagcgaagtt
    26521 gctggcaacc cattatcagc aaactaatct tgactggtat aactccagaa acacgaccaa
    26581 gctggacgaa tccgttaacc gcgtaatgcc gcaattcaaa gttgacggca aaatggtctt
    26641 tgaacgcgat atggaaatgc tggctccggg ttatacccaa acgctggaac cgcgcgcgca
    26701 gtatttgtac gtgccatatc gcgatcagag cgacatctat aactacgact catctctgct
    26761 gcaatctgac tactctggcc tgttccggga ccggacttac ggcggtcttg accgtattgc
    26821 ctccgctaac caggtgacga ccggtgtcac atctcgcata tatgatgatg ctgccgttga
    26881 acgttttaat atttccgttg gtcaaatcta ctatttcacg gagtctcgca ctggcgatga
    26941 caacataaca tgggagaatg acgacaaaac gggctcactg gtgtgggcag gcgatactta
    27001 ctggcgtatc tccgagcgtt ggggattgcg tggcgggatt cagtacgata cacgtctgga
    27061 taacgtagcg accagtaact ccagcattga ataccgtcgg gatgaagacc gtctggtaca
    27121 gctgaattac cgttacgcca gcccggaata tattcaggct acgctgccta agtactattc
    27181 cactgctgag caatataaga atggtatttc gcaggtaggt gctgtcgcca gctggccaat
    27241 tgccgatcgt tggtccattg ttggggccta ctactacgac accaatgcga acaagcaagc
    27301 cgactctatg ttaggtgtgc aatacagctc ctgctgctat gcaattcgcg tcggttacga
    27361 gcggaagctg aacggttggg ataacgataa acaacatgcg gtatatgaca acgcaatcgg
    27421 ctttaacatc gaacttcgcg gcctgagctc caattacggt ctgggtacgc aagagatgct
    27481 gcgttcgaac attctgccgt atcaaaacac tttgtgattt gttgatttac cacgtaatcc
    27541 gcagtgcggt taattgaaat ggaaaaagta tgaagaactg gaaaacgctg cttctcggta
    27601 tcgccatgat cgcgaatacc agtttcgctg ccccccaggt agtcgataaa gtcgcagccg
    27661 tcgtcaataa cggcgtcgtg ctggaaagcg acgttgatgg attaatgcag tcggtaaaac
    27721 tgaacgctgc tcaggcaagg cagcagcttc ctgatgacgc gacgctgcgc caccaaatca
    27781 tggaacgttt gatcatggat caaatcatcc tgcagatggg gcagaaaatg ggagtgaaaa
    27841 tctccgatga gcagctggat caggcgattg ctaacatcgc gaaacagaac aacatgacgc
    27901 tggatcagat gcgcagccgt ctggcctacg atggtctgaa ctacaacacc tatcgtaacc
    27961 agatccgcaa agagatgatt atctctgaag tgcgtaacaa cgaggtgcgt cgtcgcatca
    28021 ccatcctgcc gcaggaagtc gaatccctgg cgcagcaggt gggtaaccaa aacgacgcca
    28081 gcactgagct gaacctgagc cacatcctga tcccgctgcc ggaaaacccg acctctgatc
    28141 aggtgaacga agcggaaagc caggcgcgcg ccattgtcga tcaggcgcgt aacggcgctg
    28201 atttcggtaa gctggcgatt gctcattctg ccgaccagca ggcgctgaac ggcggccaga
    28261 tgggctgggg ccgtattcag gagttgccgg ggatcttcgc ccaggcatta agcaccgcga
    28321 agaaaggcga cattgttggt ccgattcgtt ccggcgttgg cttccatatt ctgaaagtta
    28381 acgaccttcg cggcgaaagc aaaaatatct cggtgaccga agttcatgct cgccatattc
    28441 tgctgaaacc gtcgccgatc atgactgacg aacaggcccg cgtgaaactg gaacagattg
    28501 ctgctgatat caagagtggt aaaacgactt ttgctgccgc agcgaaagag ttctcgcagg
    28561 atccaggttc tgctaaccag ggcggcgatc tcggctgggc tacaccagat attttcgatc
    28621 cagccttccg tgacgccctg acccgcctga acaaaggtca aatgagtgca ccggttcact
    28681 cttcattcgg ctggcattta atcgaactgc ttgatacccg taatgtcgat aaaaccgacg
    28741 ctgcgcagaa agatcgtgca taccgcatgc tgatgaaccg taagttctcg gaagaagcag
    28801 caagctggat gcaggaacaa cgtgccagcg cctacgttaa aatcctgagc aactaatggt
    28861 taaaacccaa cgtgttgtga tcactcccgg cgagcccgcc gggattggcc cggacttagt
    28921 tgtccagctt gcacagcgtg agtggccggt cgaactggtt gtttgtgccg atgccactct
    28981 ccttaccgac cgggcagcga tactcggttt gccgctcacc ctccgccctt attcccccaa
    29041 ctcccctgca caaccgcaaa ctgcgggcac attaacgcta cttcctgtcg cgctacgaga
    29101 atctgtcact gcggggcagt tagcggttga aaatgggcat tacgtggtgg agacgctggc
    29161 gcgagcgtgt gatggctgtc tgaacggtga atttgctgcg ctgatcacag gccccgtgca
    29221 taaaggcgtc attaacgacg caggcattcc gtttaccggt cataccgagt ttttcgaaga
    29281 gcgttcgcag gcgaaaaaag tggtgatgat gctggcgacc gaagaacttc gcgtggcgct
    29341 ggcaacgacg catttaccgc tgcgcgatat cgcagacgct atcacccctg cacttttgca
    29401 cgaagtgatt gctattttgc atcacgattt gcggaccaaa tttggtattg ccgaaccgcg
    29461 cattctggtc tgtgggctga atccgcacgc gggcgaaggc ggtcatatgg gtacggaaga
    29521 gatagacacc attattccgg tgctcgacga actgcgggcg caggggatga aacttagcgg
    29581 gccgctgcct gctgataccc tgtttcagcc taaatatctc gataacgccg acgcggtgct
    29641 ggcgatgtac cacgatcagg gtcttcccgt gctaaaatac cagggcttcg ggcgcggtgt
    29701 gaacattacg ctgggcctgc cctttattcg cacatcagtg gaccacggca ccgcgcttga
    29761 actggcggga cgtggcgaag ccgatgtcgg cagttttatt acggcgctta atctcgccat
    29821 caaaatgatt gttaacaccc aatgaataat cgagtccacc agggccactt agcccgtaaa
    29881 cgcttcgggc aaaactttct caacgatcag ttcgtgatcg acagcattgt ttctgccatt
    29941 aacccgcaga agggccaggc gatggtcgaa atcggccccg gtctggctgc attgaccgaa
    30001 ccggtcggcg aacgtctgga ccagctgacg gttatcgaac ttgaccgcga tctggcggca
    30061 cgtctgcaaa cgcatccatt cttaggcccg aaactgacga tttatcagca ggatgcgatg
    30121 acctttaact ttggcgaact ggccgagaaa atgggtcagc cgctgcgtgt tttcggcaac
    30181 ctgccttata acatctccac gccgttgatg ttccatctgt ttagctatac tgatgccatt
    30241 gccgacatgc actttatgtt gcaaaaagag gtggtgaatc gtctggttgc aggaccgaac
    30301 agcaaagcgt atggtcgatt aagcgtcatg gcgcaatact attgcaatgt gatcccggta
    30361 ctggaagtac cgccgtcagc ctttacacca ccacccaaag tggattccgc cgtcgtgcgc
    30421 ctggttcctc atgcaacgat gcctcacccg gttaaagatg ttcgcgtgtt gagccgcatc
    30481 accaccgaag cctttaacca gcgtcgtaaa accattcgta acagcctcgg caacctgttt
    30541 agcgtcgagg tgttaacggg aatggggatc gacccggcga tgcgagcgga aaatatctct
    30601 gtcgcgcaat attgccagat ggcgaactat ctggcggaga acgcgccttt gcaggagagt
    30661 taacgatgat caattcgccc cgagtgtgta ttcaggttca aagcgtctac attgaggctc
    30721 aatcttcacc tgataatgaa cgttacgttt ttgcttatac cgtaaccata cgcaatctgg
    30781 ggcgagcgcc agtgcagttg ttggggcgtt actggctgat caccaatggc aatggccgtg
    30841 aaaccgaagt ccagggcgaa ggagtggttg gcgtccagcc acttatcgcg cctggcgaag
    30901 agtaccagta caccagcggt gcaatcattg aaacgccgct gggcaccatg cagggtcact
    30961 acgaaatgat cgatgaaaat ggcgtccctt tcagcatcga cattcccgta ttccgactcg
    31021 ccgttcccac actcattcat taaaagaata tggcgacata ccttattggc gacgttcatg
    31081 gttgttacga tgaactgatc gcattgctgc ataaagtaga atttacccct gggaaagata
    31141 ccctctggct gacaggcgat ctggtcgcgc gcggcccggg ttcgctggat gttctgcgct
    31201 atgtgaaatc cttaggcgac agcgtacgtc tggtgctggg caatcacgat ctgcatctgc
    31261 tggcggtatt tgccgggatc agccgcaata aaccgaaaga tcgcctgaca ccgctgctgg
    31321 aagcgccgga tgccgacgag ctgcttaact ggctgcgtcg ccagcctctg ctgcaaatcg
    31381 acgaagagaa aaagctggtg atggcccacg cagggatcac gccgcagtgg gatctacaga
    31441 ccgccaaaga gtgcgcgcgc gatgtagaag cggtgctgtc gagtgactcc tatcccttct
    31501 ttctcgacgc tatgtacggc gatatgccaa ataactggtc accggaattg cgggggctgg
    31561 gaagattgcg ttttatcacc aacgccttta cccgtatgcg tttttgcttt ccaaacggcc
    31621 aactggatat gtacagcaaa gaatcgccgg aagaagcccc tgccccactg aaaccgtggt
    31681 ttgcgattcc tggccctgtc gctgaagaat acagcatcgc ctttggtcac tgggcatcgc
    31741 tggaaggcaa aggtacgccg gaaggtattt acgcgctgga taccggctgc tgctggggtg
    31801 ggtcattaac ctgcctgcgc tgggaagata aacagtattt tgtccagccg tcgaaccggc
    31861 ataaggattt gggtgaggga gaggcggtcg cgtcttaaat cccgtcgagc ataaaatcta
    31921 atgctccttt aatcaacgtt cggtgcgcgc tggcatcgca aaatttcgtt cccagcgcgt
    31981 taactggaac agttgcagtc tcatgcgtca tgagcacata ttctttgccg tctaccagta
    32041 aaaggattgg gttaagccgt tctggtgggc gaatacggct aaatcgttca ataggtgtta
    32101 atggaataac aatacggcga ttaatcacac caataatgtc gctggtaaca tcgataacaa
    32161 agggaaacgc ggcgttcctg ctgcgactgc ggtataccgt aaattgcatc gctagaagct
    32221 ccggtactca tcagagaaac atccggtttc atcagccaat tgatttaacg cagcgatagc
    32281 ttctgcgttc tcttcacgcc aacgtgttgc tgcatgtttt ttaagttcag cattgagcgc
    32341 aaccgtaagg gtggcgctca tatttaagcc tgcatcacga gcgcgattga ctaactcacg
    32401 gtcgacggtg acggtcacac tttgtgtggt acgttttgca gtcatgatat cgtgctcctg
    32461 tacaatttat acgtctagta tacacggata tctgcacgta tcagtgatgc cggaattcta
    32521 atatacaaaa ttaccgccgc tccagaatct caaagcaata gctatgagag ttctgcgcat
    32581 cagcgtcgtg gaattcgctg aataccgatt cccagtcatc cggctcgtaa tccgggaaat
    32641 gggtgtcgcc ttccacttct gcgtcgatat gcgtcagata cagtttttgc gcttttgcca
    32701 ggaactgctc ataaacgcga ccgccgccaa tcaccatgat ttctggtacg tcaccacacg
    32761 ccgcgatggc ttcatccacc gacttcaccc acgttacgcg atcgtccgta cccggttgac
    32821 tgctgaggat aatatttttg cgtcctggca acggacgacc gatggattcc caggtatggc
    32881 ggcccataat cacgggttta tttaaggtgt tgcgtttaaa ccaggcgaga tcggcaggca
    32941 ggttccacgg catggcgttt tccatgccga taacgcgatc taccgctaac gccgcaatca
    33001 gactgatcat tgagatttcc cgataaaaaa aattgtcgcc actatacgta aagcgtaaac
    33061 cgtcgtcgac tggtgcgagg atgatgttga ggaaaatttt atattctgct ggcgagtcca
    33121 cgctctcccc ctggactcgc cgcattacaa tgaaacaaaa acaaacagtt agctgtaaag
    33181 tgtgatttac gtcactcttt attaggatga gggtttcgtt tccggttcat ccgccatgtt
    33241 gccggtatgt ttaccttctt ctgttccctg ccagccatgt cgttgaatta atgacagatg
    33301 ttcgcggtcc tcggtgatga tctcacttaa catcgcgctg gtgcgtttat agaccgccgc
    33361 gcgggctttg gtgtcattct caaccattgc catctcttcc accatctgaa tattaaagcg
    33421 gcggaacaca tcggcacgtt ctcgcgcttc atacggcccc agacctaaac tttccagcgc
    33481 cagacgcccg gttttcagcg caccttcaaa ggtttcacgc tccggctttt caacgcctgc
    33541 ctgacgcaaa cgaatgtagt ggtcgacatc gcgggcgcgg gcaataatct gcaaatgcgg
    33601 gaaatgttct ttcaccatct ccgtcagttg caggttggtt tgcggatcgt caatagcgtt
    33661 aatcagcact tccgctttcg ctgctccggc agattccagt aaatccatcc gcgtggcatc
    33721 gccgtaaaac actttcatac caaatttacg cagcgtttcg atatggtccg gatcgtgatc
    33781 gagcaccacc attttcaccc cgctggagag cagtaaacgt ccggtaatct gtccaaaacg
    33841 accgaatccg gcgataatca cgcgcggctg ttcttcatcg atctcatcgg cttcacgcgc
    33901 ttcctcagta gaagattgct caaggcgatt gaggatcacc agcagaatcg gcgttgctgc
    33961 catcgacagc gccaccgcca gggtcagcga tttcgcccac tccggctcca gcacattcgc
    34021 catctgcgcc gcgccaaata ccacaaaggc aaactcactg ccctgcccta acaacaccgc
    34081 aaaccagcga cgctgtttat ttggcacctg caacggtcgg gcaatcagcc acagcatggc
    34141 gattttgatg atgaggaaac cgagcagcaa aatgacaatg cgcaatgggt tttcaatcag
    34201 cgtgccaaag tctatcgaca tgccaacacc gatgaaaaac agccccaaca gcaaaccttt
    34261 aaacggttcg atatcgcttt ccagcgcatg acggtattcc gagcttgcca gcagtacgcc
    34321 cgccagaaac gcgcccatcg ccatcgacaa gccgacctct tccagcagca aaccaaagcc
    34381 aaacacaagg aataacgcca cggcactaaa cacttcccgc aagccagagc gggcaacaaa
    34441 acgcagcgcc ggacgcgtaa catagcgccc cagcaatacc accagtacca gcgcacccgc
    34501 cacttttaac gccgagagag caaatgcgcc catcgtcgtc gaggcactgc tcgccgccag
    34561 tagcggaatc atcgccacca gcgggatcgc cgcaatatcc tggaacagca gcaccgcaaa
    34621 ggcgctgcga cccatttgcg tcaccatcag attgcgttca ttcatcgcct gcatggcaat
    34681 cgccgtagag gagagcgcca gcgtcatgcc gatcaattcc gcgacctgcc agcgcagccc
    34741 aagtaacatg cagaacagcc ccagcaggcc gccgcaaatc accatctgta atgcgccacc
    34801 gccgaacacc gccgcacgca gcttccacag cctttgtgga tcgagttcga ggccgataat
    34861 aaacagcatc agcaccacac caatttcagc aaagtgcaga atagattcgg catcggtcac
    34921 cagccgcaac ccccacgggc caataatgca gcctgcaatc aggtagccaa gtactgatcc
    34981 cagaccgagg cgtaccgcaa tgggtacaat cagcgccgcc gatccgaggt agatcagcgc
    35041 ctgaaccagc gtatgactat ccatgatggg cctcctgcca ttccagcaga cgttgcttat
    35101 agtggcgcgc ctgcccttcg agggtttcgt cgtcacaaat aaaggtgcag tgcatggcaa
    35161 acggtggcag ccagttcagc ccgcagtaga ttgccgtcgc ctgtagcggc tgcgacagca
    35221 catcaaagcc cggatgcgca ccaatttcaa aatggctttt cccgccgccg gtcgtcaccg
    35281 cccacagcaa atgtttgcca tgcagcgccg tgccgccgtg accgtaagcc cagccgtgcg
    35341 agaaaacttt atcgatccaa agtttaagga gcggtggaat gctgtaccac tgcatcgggt
    35401 gctgccagac gatcagatcg gcgcgagaca gcgcctcctg ctcggcggca atatcgatat
    35461 tgaagtcagg atagagttga taaagagagc gaatttcgac gccatccagc gtccttgcct
    35521 gttcaagcat ccgtttattc gcatgggaat gatgcggata cggatgcgca taaattatga
    35581 ggatcatagt tattagcccg cgtgtttacg cctttgtttt cccacaaagt gtagtcatat
    35641 attcacgcgg cttatagtca ataatattga ttcagaaaag agagaaatct gaacaaatta
    35701 tcccattctc tgcccgcgga tcgtcatatt gctggtctgc gccagtgaca ttcctcgagt
    35761 ctccggggca aacgctacgg aaatcaacaa gccaaacagc gagatacccg cccccattag
    35821 catcgtgtta ctgataccgt aattattgat aaatatcggc agtgcccagg tcgaaacaat
    35881 ggtgccaata cgacttaagg acataatcac gcccacggca gaggcgcgga tatccgtcgg
    35941 gaagagttca ttaggataga gccactgcaa attacccggc ccgccagaga aaaaggcata
    36001 caccgcaaag gccatcacta ccagccagat ccccatatcc gggattagcc ccaaaaccgc
    36061 cagcgccagc gtcatcatgg caaagctgcc aatcaacaat ggacgccgtc cggcagtgtt
    36121 tagccacagc atcggcggaa tacagccgag cataaagaac aggctaatca ccacattccc
    36181 tagtgccgcg tttttgccaa cccccaatcc caacaaacca acgatttgcg ggccaaaggt
    36241 gtaaatggcg aacattggga tcacctggca ggtccagatg gcggcaacaa acagaacaaa
    36301 aggaaaatgg cggcgattaa acagatcgcg aaaacgggtt tgctgcggct gctcttcctc
    36361 gaaagccacc ggttcgccaa acagttttat catcatctct tcgcactctt ttactcgccc
    36421 tttgcgtaac agccagcggg gagattctgg cagttcgaat cgaccaatca aaatcaacaa
    36481 acaagggatc gccgcgctac ccagcatcca gcgccagccg ccttccacat cataaagcca
    36541 gtagccgacc agatcggcgc aggtcgcgcc gacataccac atggcggcga taaagctgat
    36601 ggaaaatgcc cgctgacggg tactggagaa ctcggtgatc attgaggtgg cgatgggata
    36661 atctgcaccg atgacaatgc cgataagtac ccgcatcacc aacagttcga cgggggatga
    36721 aacaaacatc gtcgccaccg atatcacgcc gatggcgatg atatcaatga ggaacatttt
    36781 gcgccgtccg actttatcgg aaatataacc aaacagcgat gtgccaacga acagcccggc
    36841 gagcgttccc gcgcccagca agccaatcca gtcagcgtcc agtttcagcg ccggcgtcag
    36901 ttgctccagc gccacgccaa tcattaccag tacataacca tccagaaacg gaccaccgct
    36961 tccccacagc aaaatgcggc ggtgaataga ggagaatttg agatcgtcaa agtttctgga
    37021 cggttgcatg ggttattcct gttttttgca atgagagtca gtgcggcgtt acgtatcaaa
    37081 ccaacatcag ccgtaacgga actccacacc aaaggtgccg cgcgggtatt cccactgttc
    37141 cagcgccgac cccagcccca gaatgcgaca ggtgccgcac tccagacatc cggcgtaatc
    37201 gaagcgcaca ctgccgtcat cctgcttctt gtacagacct gcggggcacg ctttcaccag
    37261 cagctccagg gcctgtttat cggcatcggc cttcacaaca atgtgcggat gctcttcatc
    37321 gacattgaat ttattgacgc ccagtttgac gtccacattg acgggagaag tcataaaacg
    37381 gtcactcctt tcatgccatc cttgattaga ttgatgaagc ccactttctt accgtggcgg
    37441 aggattttct tgcgcatcag ttctggcgcg ctgccgtcaa tggtgaacag gtcacgcgcc
    37501 acgcccaccg ccagttccgg gtagccgcta aacatgcgtg ggttatcaag gaacgccggt
    37561 agtttctggt acatacgcat atcgcgcagc gggccactct caagatgctg acgatattcc
    37621 gcgagttttt gcttactgaa atcatcgctt ttcatcgctg aaagcacggt ttttgctgca
    37681 gcttccccgg cggcaatcgc cagatccata ccgcgaatgg taaaaccgag gttcatacac
    37741 attccggcgg catcaccggc aatcaatacg ccgtcaccga ccagctccgg cagcatgttg
    37801 atgcctgctt ccggcactac gtgagcggaa tattccacca gcttgccacc cgcgatcagc
    37861 ggtgcaacgg ccggatgttg tttgaaatct tccagcattt gcggcaccga ttttttcgcg
    37921 tcatgcagat gatgcagacc acaaaccagc cccagcgaca gggtgttttc attggtataa
    37981 agaaaaccgc cgcccatcag gccatcagta ggtgatcccg caaacaggca agccgcgccc
    38041 tgattaccct gcaactgaaa acggtcttcg attaccgact tcggtaactc gatcagttcc
    38101 ttcacgccaa ccgccacatc cgtcggtttg acgcgttttg ccatccccaa tttttcggca
    38161 aggatggagt tcaccccatc agcaaggatc accgttttcg cttcaatcac atcgccatcg
    38221 gcttctacac cgacgacttt gccatcgcgc tgtacgaggt tatcgacgcg gatcccggta
    38281 attaactgcg cacccgcttc ttcggcctgc tccatcaacc aggcatcaaa tttactgcgc
    38341 aaaacggagt aagaacgctg ggatggcgag gtttcgtcac cgttgcagta gtccatagtc
    38401 atcgctgact tttccgtcat aaacgcgagt ttttcatggg tgatcaggcg ttctacgggg
    38461 gcggagtcgg cgaaacctgg aataatgtgt tccagactgt gggcatagag acgcccgccg
    38521 gtgacgttct tggcacctgc ggaattgcca cgctcgataa ctaacacttg cgcaccttcg
    38581 cgggcgagca ccagtgcggc aaccgaaccg gcaagccctg caccgacgat gatggcgtca
    38641 aagatatctt cggacataag agctccctgt cagcggcagg gccgtaatgc agccctgcca
    38701 gagtggatca acgcgctaaa gctgcggtca gcgccggaag gatcttcacg gcgtcgccaa
    38761 caatgccgta atccgcgtac tggaagatcg gcgcattttt atctttgttg atggcgaaaa
    38821 tggtttgcga tgcattagca ccaaccatgt gctggatctg cccggagatc cccaccgcca
    38881 ggtacagttc aggtttcagc atcaggttgg agataccgac atagcgttcg tgctccatcc
    38941 atttttcgtt ttccgccacc ggacgagaac aggccaactc cgcacctatc gccttacaaa
    39001 gctgttctgc cagcgcaatg ttctctttgc tgccaatgcc gcgaccgacg ctgaccacca
    39061 gacgggcttt gtcgagatcg acgctgttgc tctggcgcgc ctgggttgcc gtgcgggtaa
    39121 tcgccacagc cggagcctgc cactccacgg tgtgcgtttc gccagtgcgt gacgcgtctg
    39181 gctgagccgc atcgaacgtg ccactgctga tggtcagtac cgcatacggc gtggcaatgc
    39241 gttcttcgcc aatcgccagg ccgccgtaaa ccatgtgttt cactgttgct ttaccgtcct
    39301 gtacgctgac ggtgctggca tcgttagaca ccgccgcttt aaggcgataa cccagttttg
    39361 ccgccagtaa tttgccgcga cgggtgtttg gcaacagcac caggccgtct gtgccgtgct
    39421 ggcgaatagt gtcagccatg acactggcgt aatcttcgat catccgatcg tccggtttgc
    39481 cgtttaattt ccagacatga ttagcgccga gctggattgc ctgtgcgccg tcggcatcat
    39541 tgaggacaaa ggtgttgatt tgattagcta aagcctgcgc accgttcatc agttccggca
    39601 gacgagaagg ggtatcgctg aatacccaga cttgagaaaa cgtgttcata gcatcccctg
    39661 taattaaatg actttgcgaa gattttcagc aaatgcggcg atctgttctt cgccgtcgcc
    39721 ttcaatcacg atgcgctgac gctcgcgctg tttcggcgcg gcaacctgtt gttctgacca
    39781 ggctgcctct gcgttaaaac cgatatccgc cgccgaccat acctggactg gctttttcgc
    39841 cgcgccgaga atggctttca tcgaaggaat ttgtggggag ttgatatcag tggaaacagc
    39901 aacaaccgca ggcagcggaa tgcttaaggt ttcggtttca tcttccagtt cgcgctcaac
    39961 ggtgagggta tctgccgtca gggagataat tttgctgacg ccgttaactg ccggaatatt
    40021 gaggatttcg cccaccagca gaccaacctg ctgggcataa aggtcggaag aaccatcgcc
    40081 acagaggatc agatcaaagc ctgctttctg ggcggctgca gccagtgcgc tcgccgtttg
    40141 ttgcggcagt gcctgctcga actggtcatc aatcaccaca atcagttcat ccgggccgcg
    40201 cgatagcaca tctttacgcc ctttggcgtt ggtcagggct ttaccgccca cacttaaggc
    40261 tgtcacctgc gcctctgctg cctgttgctt tagctggcaa gccgcttcaa tagcgttgag
    40321 atcgtattgg cttattttgg catcggcttt gctgaagtct aatgaaccat cagcattatt
    40381 gaccgcaata tcctgttcat caggcacgca cttatagcaa gtaataatct tcattgcatc
    40441 tccagaaatc atgaaggaaa cgtcgttcat atcggtggtg ttaattacag atatcacgcc
    40501 ctttaatttg gaaataaaac tttaatcacc aatattgaaa atgtcacacg cagataaatc
    40561 caactttcaa tattgttaag ttcctcacca atattgaaaa catggcgta
//
LOCUS       DAZFKF010000038        39981 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFKF010000038 DAZFKF010000000
VERSION     DAZFKF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.038"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(238..753)
                     /gene="pgpA"
                     /locus_tag="ACHKA1_002400"
     CDS             complement(238..753)
                     /gene="pgpA"
                     /locus_tag="ACHKA1_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO5680040.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(731..1708)
                     /gene="thiL"
                     /locus_tag="ACHKA1_002401"
     CDS             complement(731..1708)
                     /gene="thiL"
                     /locus_tag="ACHKA1_002401"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO5680041.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1786..2205)
                     /gene="nusB"
                     /locus_tag="ACHKA1_002402"
     CDS             complement(1786..2205)
                     /gene="nusB"
                     /locus_tag="ACHKA1_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO5680042.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2225..2695)
                     /gene="ribE"
                     /locus_tag="ACHKA1_002403"
     CDS             complement(2225..2695)
                     /gene="ribE"
                     /locus_tag="ACHKA1_002403"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO5680043.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2784..3887)
                     /gene="ribD"
                     /locus_tag="ACHKA1_002404"
     CDS             complement(2784..3887)
                     /gene="ribD"
                     /locus_tag="ACHKA1_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO5680044.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(3891..4340)
                     /gene="nrdR"
                     /locus_tag="ACHKA1_002405"
     CDS             complement(3891..4340)
                     /gene="nrdR"
                     /locus_tag="ACHKA1_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO5680045.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4491..5030
                     /gene="yajI"
                     /locus_tag="ACHKA1_002406"
     CDS             4491..5030
                     /gene="yajI"
                     /locus_tag="ACHKA1_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001430049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO5680046.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHNVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLDFVRIHDIQPVMH"
     gene            5329..6213
                     /gene="tsx"
                     /locus_tag="ACHKA1_002407"
     CDS             5329..6213
                     /gene="tsx"
                     /locus_tag="ACHKA1_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO5680047.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6403..6750)
                     /gene="yajD"
                     /locus_tag="ACHKA1_002408"
     CDS             complement(6403..6750)
                     /gene="yajD"
                     /locus_tag="ACHKA1_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO5680048.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(6879..7850)
                     /gene="secF"
                     /locus_tag="ACHKA1_002409"
     CDS             complement(6879..7850)
                     /gene="secF"
                     /locus_tag="ACHKA1_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO5680049.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(7861..9708)
                     /gene="secD"
                     /locus_tag="ACHKA1_002410"
     CDS             complement(7861..9708)
                     /gene="secD"
                     /locus_tag="ACHKA1_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO5680050.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(9736..10068)
                     /gene="yajC"
                     /locus_tag="ACHKA1_002411"
     CDS             complement(9736..10068)
                     /gene="yajC"
                     /locus_tag="ACHKA1_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO5680051.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(10091..11218)
                     /gene="tgt"
                     /locus_tag="ACHKA1_002412"
     CDS             complement(10091..11218)
                     /gene="tgt"
                     /locus_tag="ACHKA1_002412"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO5680052.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(11273..12343)
                     /gene="queA"
                     /locus_tag="ACHKA1_002413"
     CDS             complement(11273..12343)
                     /gene="queA"
                     /locus_tag="ACHKA1_002413"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO5680053.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            12436..13017
                     /gene="acpH"
                     /locus_tag="ACHKA1_002414"
     CDS             12436..13017
                     /gene="acpH"
                     /locus_tag="ACHKA1_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO5680054.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVITILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYDALETRFWQFYPRMMAQASRKAL"
     gene            complement(13038..14519)
                     /locus_tag="ACHKA1_002415"
     CDS             complement(13038..14519)
                     /locus_tag="ACHKA1_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680055.1"
                     /translation="MTISNSNLLKPFITGAELPPFKEDVFSKTEEQFKKSTHGKSLFI
                     CGNENLVNISAEKIDEIKNSKNFFSNVVNIIILLIKYPDKILPILHGCPAESIELIKN
                     AKRTGQWSYEMTDKNGQNIEVSYIFTDTIDDKSDGKLCLKICNSCNQNDSSTHEYKID
                     HQQLKNHIRQLEEWALLEKFYDNNAYYSKRTKQLEKKIAKAGFITGKVNAEKFNVKEF
                     KDDITSMLCEQPPMCTHQISSSGYLSADKVPDLYKTNLKDAQRDLKTTPVDIDGVSLK
                     SKTREKIAEEIAKKTAEKTAEKTAKKNSEIIAKQNANSIEEKNIEKNNKETNPEDNFV
                     LKFSQEEINEMLLDYSDEEQKKLRPFFTSDHIYCLFDAFSQCLYNVARDGSPRYAGLL
                     QQIASKCAILQDFDQQLFWRTFSGGVFHRRISVNSEPEKTDVWRFSTQFDFPHINDNG
                     ELSRTVLMFEGVAESNFKDIANHSSECFSYRCYAAPLDANAKK"
     gene            complement(14707..16524)
                     /gene="malZ"
                     /locus_tag="ACHKA1_002416"
     CDS             complement(14707..16524)
                     /gene="malZ"
                     /locus_tag="ACHKA1_002416"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO5680056.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAIDVPDIGSQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(16680..18053)
                     /gene="proY"
                     /locus_tag="ACHKA1_002417"
     CDS             complement(16680..18053)
                     /gene="proY"
                     /locus_tag="ACHKA1_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO5680057.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(18129..19448)
                     /gene="brnQ"
                     /locus_tag="ACHKA1_002418"
     CDS             complement(18129..19448)
                     /gene="brnQ"
                     /locus_tag="ACHKA1_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO5680058.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(19855..21150)
                     /gene="phoR"
                     /locus_tag="ACHKA1_002419"
     CDS             complement(19855..21150)
                     /gene="phoR"
                     /locus_tag="ACHKA1_002419"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO5680059.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(21208..21897)
                     /gene="phoB"
                     /locus_tag="ACHKA1_002420"
     CDS             complement(21208..21897)
                     /gene="phoB"
                     /locus_tag="ACHKA1_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO5680060.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            22087..23289
                     /gene="sbcD"
                     /locus_tag="ACHKA1_002421"
     CDS             22087..23289
                     /gene="sbcD"
                     /locus_tag="ACHKA1_002421"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO5680061.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELEESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            23286..26429
                     /gene="sbcC"
                     /locus_tag="ACHKA1_002422"
     CDS             23286..26429
                     /gene="sbcC"
                     /locus_tag="ACHKA1_002422"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO5680062.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTVPQEDEEESWLATRQQEAQSWQQHQN
                     ELTALQNRIQQLTPILETLPQSDELPHCEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALVDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPGGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            26555..27739
                     /gene="araJ"
                     /locus_tag="ACHKA1_002423"
     CDS             26555..27739
                     /gene="araJ"
                     /locus_tag="ACHKA1_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO5680063.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(27873..28781)
                     /gene="mak"
                     /locus_tag="ACHKA1_002424"
     CDS             complement(27873..28781)
                     /gene="mak"
                     /locus_tag="ACHKA1_002424"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO5680064.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            28906..29817
                     /gene="rdgC"
                     /locus_tag="ACHKA1_002425"
     CDS             28906..29817
                     /gene="rdgC"
                     /locus_tag="ACHKA1_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO5680065.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(29895..30350)
                     /locus_tag="ACHKA1_002426"
     CDS             complement(29895..30350)
                     /locus_tag="ACHKA1_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680066.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(30347..32626)
                     /locus_tag="ACHKA1_002427"
     CDS             complement(30347..32626)
                     /locus_tag="ACHKA1_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HFO5680067.1"
                     /translation="MNRINILVICMVLFFMTGYACATEWISSEDLITSDFHLMTADER
                     NVVKAATDDSMEAAYMLKDNIRWYYHNGNLSLPANFSNQNKLVVNGNLTISGDYDDYL
                     SGNGHLIVLGNVIVDNFINHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARGIIVS
                     DKATQFEVIKAEFYINEDGSGEGYNWDENIQKAYSLVTADLYDHTEIETDNISNAYPD
                     YDSVADNIVQGLPLFRDKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFLTHTE
                     SLSPAVMLQLLQHPDDQTRESMAQSWPAQQMHLLTDELIKDEAVARGLVKNSNISADV
                     NKKLMSVPVESVQLEQARQDNLSPDIVASLSHSPFLNVRKTLLSHYDYAWLVPTAVAD
                     ELINSEDPELRERITGADLTAQQAVLLSKDKSLKVREALARTLTELKITQLSATLRTE
                     DIERIAEQMYLDNKENKNIVKALLIALPEMRQLSLAKEDVHNLREGTRYLTSKEVISY
                     LLTQHDVPTVWDELARDKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLALIDNG
                     VVDEEMLNNAIDLLVDLPAEYRYRMRNQLFDNKELPSGIINKLDQQYRFNSDWALAVV
                     SMKNSTRRQSERGLHRWNHEDSDIFAELATIKDKSDDEWWRALLQSRNDHLRQTALRN
                     AHTPASLLTTLTEPQDRSLAINNPQLAADVKTAWLKEDPSLLLFVEQPDLSLLRDLVK
                     TGATRKIRSEARHRLEEKQ"
     gene            complement(32770..33054)
                     /gene="ppnP"
                     /locus_tag="ACHKA1_002428"
     CDS             complement(32770..33054)
                     /gene="ppnP"
                     /locus_tag="ACHKA1_002428"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO5680068.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(33126..33803)
                     /gene="aroM"
                     /locus_tag="ACHKA1_002429"
     CDS             complement(33126..33803)
                     /gene="aroM"
                     /locus_tag="ACHKA1_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO5680069.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(34061..34252)
                     /gene="yaiA"
                     /locus_tag="ACHKA1_002430"
     CDS             complement(34061..34252)
                     /gene="yaiA"
                     /locus_tag="ACHKA1_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO5680070.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(34302..34826)
                     /gene="aroL"
                     /locus_tag="ACHKA1_002431"
     CDS             complement(34302..34826)
                     /gene="aroL"
                     /locus_tag="ACHKA1_002431"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO5680071.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(35009..35467)
                     /gene="yaiI"
                     /locus_tag="ACHKA1_002432"
     CDS             complement(35009..35467)
                     /gene="yaiI"
                     /locus_tag="ACHKA1_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO5680072.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            35587..36396
                     /gene="proC"
                     /locus_tag="ACHKA1_002433"
     CDS             35587..36396
                     /gene="proC"
                     /locus_tag="ACHKA1_002433"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO5680073.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRSQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(36413..37528)
                     /gene="adrA"
                     /locus_tag="ACHKA1_002434"
                     /gene_synonym="dgcC"
     CDS             complement(36413..37528)
                     /gene="adrA"
                     /locus_tag="ACHKA1_002434"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO5680074.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(37630..37950)
                     /gene="psiF"
                     /locus_tag="ACHKA1_002435"
     CDS             complement(37630..37950)
                     /gene="psiF"
                     /locus_tag="ACHKA1_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO5680075.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(38069..39484)
                     /gene="phoA"
                     /locus_tag="ACHKA1_002436"
     CDS             complement(38069..39484)
                     /gene="phoA"
                     /locus_tag="ACHKA1_002436"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO5680076.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTADQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFNGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(39585..39845)
                     /gene="iraP"
                     /locus_tag="ACHKA1_002437"
     CDS             complement(39585..39845)
                     /gene="iraP"
                     /locus_tag="ACHKA1_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO5680077.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT        10025 a        10556 c         9666 g         9734 t
ORIGIN      
        1 gatgatattt ttcgtcaaca gtcagagaat atgagcaatt attatgagtt cgtcgactga
       61 cgatatttca gtgtcattga attatgagtg acaaagatac accttgaaat atcgccgcat
      121 agtatattcg gcgatgtcgg gttttacttt aacagagtgc cccaaaattg gggcactgtt
      181 catttataaa tcaaaccggg tggtctggaa tcgcctacac ctcccggtca atggcattta
      241 aagaataccc agcggccagt gatgaccgat aaagtacagg atgcctgcgg aaatcacccc
      301 ggcgacaata tcatcgatca tgatccccat gccgccatgt acattgcgat caaaccagcg
      361 gatcggccac ggcttccaca tatccagaat acggaaaatc acaaacccgg cggcgaccca
      421 ctgccagtca ttggtcggca gcgccatcag ggtgatccac atgccaataa actcatccca
      481 gacgatgcta ccgtgatcgt gcacacccat gtctttcgcc gtttgatgac aaagatagac
      541 gccgatacag atccccaaca tcaccaccag cgagtagagc tgccagggca aaaaggtcat
      601 cagataccag aacggaatcg ccgccagcga tcccatcgtg ccaggaacga ttgggcttaa
      661 tccacttccg aatccgacag caagcagatg ccacggatta ctcatcttca ggcgactttt
      721 cgcgacatct ttatggcgtg gcaaaatggt catatccttt ccagtctaac gtgacaggtt
      781 cgccgtcacg aataaaacaa agcccttcga tatcggcggt catttgcccg atacaggtaa
      841 acggtacgcc cagatgaccg agagccacat ccagcgcgcc acggttcagt tccggcaccg
      901 tgaaacacaa ctcgtaatct tcaccgccag agagcgccca gcgcagcgcc tgttccggta
      961 caacatggcg agaaagcgca tcagaaaacg gcagcaatgc caggtcaata cgtgcgccgc
     1021 agtcgctggc tttcacgata tgcccgagat cggaaatcag accgtcagag agatcgatag
     1081 ctgaatttgc cagatcgcgc agtgcctgcc cctgtaaaat acgcggcgat ggacggagat
     1141 gacgtttgat caagtagtcc gcatctttag catcggcaac ctgcaaacgg ttttgcaaaa
     1201 tcgccagccc ggcggcgcta tcgcccggtg taccggtcac atagatccag tcacccggtt
     1261 tcgccccaga gcgcgttaag gctcgtccca tcggaacaaa gccgtggata cccaacgtca
     1321 ttgataatgg cccacgcgtg gtatcgccgc caatgagttg catatcgtaa taattgagaa
     1381 gatcaaacaa actgtcgctg aaggactcaa gccacgcttc gtctacgtcc ggtaaggtta
     1441 atgccagcgt cagccaggcc ggatcggccc ccatcgctgc cagatcgctt aggttcaccg
     1501 ccagtgcttt ataagccaga tcagcaggat cgatatcagg gaggaaatgg ttgcccgcca
     1561 ccagcgtatc agtgctgatc gccagggtct gtttctcggg gatattgaga agagcgcaat
     1621 cgtcgccgat gcccagttcg acatcaagac gagaacttct tacacggtca aaataacggg
     1681 caatcaggga gaactcgcca catgccatac gttatgcctc agcaggtaaa aaagaaaagg
     1741 ccggggatcg cggaacagtc ttccgtgaat ctaccggcct ggatattact ttttattagg
     1801 gcgaatcaca ggtgctgctt tatcgagtac gccgttgacg aacttatggc tgtcttctgc
     1861 gccgaacgat ttcgccagtt cgatcgcttc gttaatggcc actttgtatg gcacatcgct
     1921 acgtttagac agttcgtaca gcgcaatgcg cagtactgct ttttctacct gtcccagttc
     1981 ttccagcagg cgggacaggt atggcttcat cagtccgtcg aggtatgcgg tattagtcgc
     2041 caccccggcc agcagctcac ggaagtacag gacgtcaacg tcttttacat cctgttcagc
     2101 caggaactgg tattcaacat cagcgatgtc gttctgggac aactgccagg agtagagcgc
     2161 ctggacggca cactcacgag cgcggcgacg agcagcaggt ttcacggatt tccccttact
     2221 aatttcaggc cttgatggct ttcaatacat taatcatttc aagcgcggtc agtgcagctt
     2281 ctgcaccttt gttgccagct ttggtgccag cacgttcgat cgcttgttca atgctttcag
     2341 tggtcagaac cccaaaagca accggaattt cgctgtcctg ggcaacatgc gccaggccgt
     2401 tgcttgcacc accagcgaca tattcaaagt gggcagtgcc accacgaata accgtaccca
     2461 gcgcaatcac cgcgtcgtat ttaccggttt tagccagtgc acccgccgcc agcggcagct
     2521 cataggcacc aggcacccaa acaacggtaa tgttttcatc ttttacctga ccgatacgtt
     2581 tcagtgcgtc aattgcacct tccagcaggc tgtcattgat aaagttgttg aaacgcgcga
     2641 tggtgatggc gacgcgagcg tccggggtag caacgttagc ttcaataatg ttcattttct
     2701 tccttcgggt tcgagtatgg ccccgcaggg gggcggattt tagcataata tttcgtgcgc
     2761 tgcttccctt tcgagccggg agatcatgca cccactaaat gcaggcaaac atccgggcct
     2821 acatgacgta tctctttgaa tttaaattgg ggggcgtcgg ctaatttctc aagccctggc
     2881 agcgagcata atccacgggc gtcgctgcct aatagtttag gtgcgatata gacaatcagc
     2941 tcatcgacta aacccgcctg cagcaatgcg ccagcgagcg ttggccccgc ttccacccag
     3001 atgctgttaa tctgctgttt acccagttgc atcatcagta caaccagatc cagatgacct
     3061 ttatgctctg gaatcagcaa ggtacgcacc gtttccggcc actcacgaga atcttcctgc
     3121 gtacgcgcga accaggtttc gccgggctgc tgcacaatgt gatgttccgg cgtcacgcga
     3181 ttttggctat caatcacaat acgtaccggc tggcggagat tttgttgcgg gtagagcgcc
     3241 tgagtttgtt catccagttc ggaccaacgc accgttaagg caggatcatc cgccagcacc
     3301 gtggcgctgc tggttaaaat ggcatgactt tgcgcgcgca gtcgttgtac atcacgccgc
     3361 gcctggggcg aagtgatcca ctggctttcg ccgctcgcca tcgccgtgcg accatcaagc
     3421 gatgcgccaa gttttaactg aatataagga aagccggtgc gcatccgttt gagaaagcct
     3481 ttattcaatt gctcggcttc actcatcatc agcccgtggc tgacgtcaat gccagcctgt
     3541 tgcagacggt aaagtccacg tccagcaacc tgcgggttcg ggtcctgcat cgcagcgacc
     3601 acgcgcgcta cgccagcggc gatgagtgcg tcacagcacg gtggcgtacg accatgatgg
     3661 ctacagggtt cgagtgtgac ataggcggtc gcacctttgg ctttttcacc cgccatacgc
     3721 aacgcgtgta cttcagcatg tggttcaccc gctcgttggt ggtaaccttc accgacaatt
     3781 tcgccatctt tgacaatgac gcacccgaca ttcgggttgg ggtgggtggt aaaacgtcct
     3841 cgttgcgcca gctttagcgc ccgcgccatg taatactcgt cctgcacggc ttagtcctcc
     3901 aggcgcgcga tctcttcgcc aaattctttg atatcttcga aactgcggta gacagaggca
     3961 aaacggatat aggcgacttt atcgagcttt ttcaattgct ccatcaccag attgccaatc
     4021 atcttgctcg gcacttcgcg ctcaccggtg gcgcgcagct gcgatttaat atgattgatt
     4081 gccatttcga cgtcatcgga actcaccgga cgtttttcca gcgcccgcag cattccgcta
     4141 cgtaattttt cttcattaaa cggttcacgc acgtcgttgc tttttacaac acgcggcata
     4201 accagctccg ccacttcaaa ggtggtgaaa cgttcattac acaccagaca ctgccgacgg
     4261 cggcgtacgg atgaaccctc gcccacgaga cgagagtcaa ttaccttagt gtccacggcg
     4321 aaacagaatg ggcaatgcat acggcgtcct gaccaggtgg ttaacagaaa tctattttac
     4381 cctgaactga cgatacaaca aaggcgcaac gcttttgaga taaaggatcg atgctttcgc
     4441 tgcttttacc ctctaccatt aggctaattg cgacgagaaa gggacgacct atgaatacta
     4501 acgtttttcg actgctcctg ctgggaagcc tgttcagcct tagcgcctgt gtgcagcaaa
     4561 gcgaagtgcg acagatgaaa cacaacgtca gcacgctgaa ccaggagatg acgcaactca
     4621 atcaagaaac agtcaaaatc actcagcaaa acaggctgaa tgcaaaatcc agcagtgggg
     4681 tttaccttct gcctggagcg aaaacaccgg caagactgga aagccagatc ggtactttac
     4741 gtatgtcgct ggtgaatatt acgcctgata cagatggcac caccctgacg ctacgtattc
     4801 agggcgagtc gaatgatccc ttgcccgcat tcagcggaac cgttgaatat gggcaaattc
     4861 agggaacaat agacaacttt caggaaatca atgtgcagaa tcaattgatt aatgcccctg
     4921 ccagcgtcct cgcccccagc gatgttgata ttccgttaca gttaaagggg atttctgtgg
     4981 atcagttaga ctttgtacgt atccacgaca ttcaacctgt tatgcattaa acgtattgcg
     5041 gggcaatttt gtgcgtcccg caacatcttt ccccgtcatt ttgttactcc gcttacatca
     5101 cccagattga tagtaaaagt ttgcaacaag ggtgaaagtc agtacaatcc ctgcccgaat
     5161 gtgtgtaaac gtgaacgcaa tcgattacgt aaatgataga actgtgaaac gaaacatatt
     5221 tttgtgagca atgattttta taataggctc ctctgtatac gaaatattta gaaacgcaat
     5281 ttgcgccttt ttcactcccg caagggattt tcaaacagtg gcatacatat gaaaaaaaca
     5341 ttactggcag ccggtgcggt actggcgctc tcttcgtctt ttactgtcaa cgcagctgaa
     5401 aacgacaaac cgcagtatct ttccgactgg tggcaccaga gcgttaacgt tgtcggaagc
     5461 tatcacaccc gttttggacc gcagatccgc aacgatacct accttgagta cgaagcattc
     5521 gctaaaaaag actggttcga cttctatggt tatgcggatg cgccggtatt cttcggcggt
     5581 aactccgatg ccaaaggtat ctggaaccac ggctctccgc tgtttatgga aatcgaacca
     5641 cgtttctcca tcgacaagct gaccaatact gaccttagct tcggtccgtt caaagagtgg
     5701 tacttcgcga acaactacat ttacgacatg ggtcgtaata aagatggtcg ccagagcacc
     5761 tggtacatgg gtctgggtac cgatatcgac actggcctgc cgatgagcct gtccatgaac
     5821 gtctatgcga aataccagtg gcagaactat ggcgcagcga acgaaaacga gtgggacggt
     5881 taccgtttca aaattaaata ctttgtgccg attaccgatc tgtggggcgg tcagctgagc
     5941 tacatcggct tcaccaactt cgactggggt tccgatttag gggatgacag cggtaacgca
     6001 atcaacggta ttaagacccg tactaataac tctatcgctt ccagccatat tctggctctg
     6061 aactacgatc actggcacta ctctgtcgta gctcgttact ggcacgacgg tggtcagtgg
     6121 aacgacgatg cagaactgaa cttcggcaac ggcaacttca acgttcgctc taccggctgg
     6181 ggtggttacc tggtagtagg ttacaacttc tgattatgaa aatgccggga tttattcccg
     6241 ggatttattc ccggcatttc agattgttaa caacgtgcgc tttgttcatg ccggatgcgg
     6301 cgtaaacgct ttgtccggcc cacaaaagca cgtaaatgca atatattgct gaaataacgt
     6361 aggcctgata agcgtagcgc atcaggcaat tttacgtttt aatcacttct tcttgttcat
     6421 catcgctttc aggtcagcga atgggttgta cttcgcttca ccgacatctt tctgcgcatc
     6481 ttcccctgcg ataacggtcg taccatactg atccgcttcg gtatatttcg aatgctcatg
     6541 atcgtggcaa tagagacaaa gcaattccca gttactgcca tcttccgggt tattggtatg
     6601 gtcgtgatca atgtggtgaa cggtaagttc acgcaggttg gaataaacaa actcgcggga
     6661 acagcgaccg cagacccacg gatagatttt taatgctttt tcgcgatagc cgctttctaa
     6721 ccgcgcatag ttttttggga tgatagccat gaaaagttac ctggatgaaa tgtagggtta
     6781 cgcgcagttt cccaagatgc ggggattttc gcaatctcca tacatcaaaa aaaatcccga
     6841 tcttctgacc gggattttca acatcaacgg gaacttgatt aaggcagaat tgacggctga
     6901 tccgcccctt ctttttccac tttctgctgc aacatgtgtt cgcgcttcat acccagtttc
     6961 agagccaacg cggatgccac atagatggaa gatgcagtac cgatggaaac accaatcagc
     7021 atggtcagcg agaagccttc cagtaccggg ccaccgaaga ggtacagcat caggataacc
     7081 atcaaggtag taccggatgt gatcaaggta cgatgcagcg tctgggtcaa ggacacgtta
     7141 aagatttcgt aaggcgtacc gcgacgtatc ttgcggaagt tttcacgaat acggtccgat
     7201 accacgatac tgtcgttaag cgagtaaccg ataaccgaca tcaacgatgc cacaatggtc
     7261 aggtcaatct cgatatggaa taacgacaaa atacccagcg taatgataac gtcgtgcgcc
     7321 agcgcaataa ccacccctgc cgccagtcgc cactcaaagc ggaaacctac gtacacgagg
     7381 atagacagca gcgctgccat caacgccatc gcaccggttt gcgcaaggtc tgcccccacg
     7441 ctcggaccga cgaactcaat acgcttcact gctgcattct gattggtgga ttcgttaatc
     7501 accttcagaa cctggctgcc cagcacctga ccgccggttt cgccttcagc aggcggcata
     7561 cggaccatga tgtcgtggct gctaccaaag ttttgcagca ttggctcttc aaaaccggct
     7621 ttttgcaatg catcacgcat tacgtcaatt tcagccggtt tttcgagcgt aatttcgata
     7681 accgtaccac cggtgaaatc cagcccccag ttaaagccgc gcacgcccat aataacgata
     7741 gcagcgatta acagcagacc agagatgccg aaagcccagt agtcccagcg cataaagtca
     7801 tagactttac ggccgtggtt tagttgttca acagtatatt cctgtgccac atcgcactcc
     7861 tcagattgac agcttcttga cgcgcttgcc gccatatagc aggtttacga tggcacgcgt
     7921 accgacaatc gcggtaaaca tcgacgtcgc cacaccgata ccggtagtaa tcgcgaaccc
     7981 tttaattgcc ccggtaccca ctgcgtacag gatgatgact ttaatcagcg tggtgatgtt
     8041 cgcatcgaag atagaactga atgcgccacg ataaccttca tcaattgcct gttgaacagt
     8101 acgtccgttg ctcaactctt ctttaatacg ttcgttgatc agtacgttcg catcgaccgc
     8161 taccgcaagg gttaagacga tacccgcgat acctggcata ctcagtgttg cgcctggcaa
     8221 cagcgacata ataccgacga tcaagatcaa gttggcaatc agagcactgg tcgcaatcag
     8281 accaaacttc ttatagaaga tgatcatgaa cagaatagaa accagcagac cggcaaggca
     8341 agcctccagc ccctgctcaa tgttctgcat acccagggtc gggccaattg tacgttcttc
     8401 aacaatctga atcggcgcga tcaacgcacc ggcacgcagc agcagtgaca gctgacgggc
     8461 ttcgttcggg ttgttgatgc cggtgatacg gaagctgtta ccaagacgag actggatgtt
     8521 ggcgatgtta atcacctctt cctgtttcac cagaaccgca cgaccatttg catctttctt
     8581 accgctgtct ttgtactcca caaacagggt tgccatcggt ttgccgatgt tgtccttggt
     8641 gaagttagac ataatgttgc caccagcgct atcgagcgag atgttaacct gcggctggtt
     8701 gtattcgtcc tggctggaag tggagtcggt gatatggtca ccggtcagaa ttacgcgttt
     8761 gtacagcaca accggctgac cttcgcgggt ctgtttcact tcagagtcgc ccggtacgcg
     8821 accggatgcc gcagcggcct gatcaacgtt ggtgtttacc agacggaatt ccagcgttgc
     8881 cgtcgcaccc agaatctctt tcgcacgcgc agtgtcctga atacctggca gttcaacaac
     8941 gatacggtca gcaccctgac gctgaaccac cggctccgcc acgccaagtt ggtttacacg
     9001 gttacgcagg atattgatgt tctgctgcac cgcatattca cgcgcttcac tcagacgagc
     9061 atcgctcata accgcacgca gctggttgct gccctggcta ctaatcacca ggtccggatg
     9121 gcgcttgctc agatacgcaa tggcttcatc acgagcttta gcatcgcgga aagtgatgct
     9181 caggccgtag ttgttttctt tacgaacggt ggtatacggg atgccttttt cgcgcaggtc
     9241 gctgcgcagg ctatcgatat tttgttcctg gagtttgcca agcgcggtgt ccatatccac
     9301 ttccatcagg aagtgaacgc cgccacgcag gtcaaggccg agcttcatcg gctcagcgtg
     9361 aatagctgcc agccagcgcg gcgttgccgg ggcaaggtta agcgccacga cgtatttgtc
     9421 acccataacg cccattaatg cttcacgagc gcgcagctgg gtgtcagtgg agtcgaagcg
     9481 cgcaagaata gcgccctctt ccagtgccac agacttagca gttatttttt cttcttgtaa
     9541 cgttttctgg acctggatca gcgtttgctc actggcggcg actccgcgcg caccagtgat
     9601 ctgaacagcc ggatcctcac caaacaggtt gggaagcgca tacagcagac cgatgacaat
     9661 caccacgatc agcatgacgt acttccacaa aggataacgg tttaacacgg caattccctt
     9721 agggaaaaat tttaattaca gcgccttcat ggtgcctttc ggcaggacgg cagctacgaa
     9781 gtcacgttta ataactactt cagtggtgtc attcagcgcg atagcaatgt agccgttttc
     9841 cgctactttg gttacgcgac cgaccaggcc accgttcgtc agaacttcat cacctttggc
     9901 aatggagtcc atcagctttt tgtgttcttt ggtgcgcttc tgctgtggac gcaggatcat
     9961 gaaatagaaa atcagaccga ataccaccag catcaaaatc aaagacatcg ggctaccttg
    10021 cgccggtgca cccgttgccg ctaccgcatc agaaataaaa aagctcatta aatttccctc
    10081 attattaata ttaatcaacg ttcaaaggtg gtacttctcg cccctgacgc tggtaaaaat
    10141 cagttacgaa gctctctaat ttaccctctt caatagcctt gcgtaaaccc gccatcaaac
    10201 gctggtagta acgaaggtta tgaatggtat tgagtcgcgc gcctaatatt tcgttgcaac
    10261 ggtcaagatg atgcaagtaa gcgcgtgaat aattgcgaca ggtgtagcaa tcacactcag
    10321 gatcgagtgg gccagtatcg ctcttatact tcgcattgcg gattttcacc acgccatcgg
    10381 tcacgaacaa atgaccattt cgggcgttgc gggttggcat tacgcagtca aacatatcga
    10441 taccacgacg tacgccttca accaggtctt ctggtttacc aacgcccatc aggtaacgcg
    10501 gtttgtctgc cggaatttgc gggcatacat gctccagaat gcggtgcata tctgctttcg
    10561 gctcacccac agccagaccg ccgacagcgt agccatcaaa accgatatct accagacctt
    10621 taacagaaat atcacgtaaa tcttcgtaaa cgctgccctg aatgatacca aacagcgcat
    10681 ttttgtttcc gagactgtca aaacgctcac ggctacgctt cgcccaacgc agagacatct
    10741 ccatggagcg ttttgcgtaa tcccagtcag caggatacgg cgtacactca tcaaagatca
    10801 tgacgatatc cgaaccaaga tcgtactgaa tctccatcga cttttccggg tcgaggaaaa
    10861 tcgggtcgcc gttgatcggg ttacggaagt gcacgccctg ttcggtgatt ttacgaatat
    10921 cgccaaggct gaagacctgg aagccgccgg agtcggtgag gatcggtccc ttccactgca
    10981 taaaatcgtg cagatcgccg tgcagtttca tgatttcctg gcccgggcgc agccacaggt
    11041 ggaaggtgtt gccgaggata atttgcgcgc cagtggcttc aacttcttct ggcgtcatcc
    11101 cttttacggt gccgtaggtg ccaacaggca taaaacaagg cgtttccact acgccacgat
    11161 caaagaccag gcggccacgg cgtgcgcgac cgtcggtggt gtccagttca aatttcattt
    11221 tttctccact acgtcagaaa aacagtccaa cgtttaaacc agcgccgcgg aattactccc
    11281 cgacgcgctc attaattgcc tgcggattgt acgtgataaa catcgcatca ccgtaactaa
    11341 aaaagcgata tttctcttct accgccgctt tataggcatt catggtgtgt tgataaccgg
    11401 caaaggccga aaccagcata atcagcgtcg actctggcaa gtggaagttt gtcaccagcg
    11461 catcgaccac tttgtactgg aagcccggat agataaagat ttgggtatcg tcaaagaacg
    11521 gttcgatgag atcgtttttc gccgcctgag ccgcgctttc cagcgaacgt actgaggtag
    11581 tgccaaccgc aatcacccgg ttaccgcgcg ctttcgccgc cagtaccgcg tctaccacat
    11641 cctgcggtac ttcagcgtat tccgagtgca tgatgtgatc ttcaatggtg tcgacgcgca
    11701 ccggctggaa ggtgcccgca ccaacgtgca gggtgacgaa cgccatctcc acgcctttgg
    11761 cgcgtaattt ttccagcaaa ggttcgtcaa aatgcagacc agcggtcggg gctgcaaccg
    11821 cgcccggttt ttcgctataa acggtttgat aaagttcgcg gtcagcgtct tcgtccggac
    11881 ggtcgatata cggcggcagc ggcatatggc cgatgctgtt gagaatatcc agtaccgagc
    11941 gttcatcatt aaattcgact tcaaacagtg cgccgtggcg cgcggtcatt gttgcgttga
    12001 tactttcgtc atcgcccagc aacagttctg cgccaggttt tggcgctttc gaggcgcgaa
    12061 tatgcgcaag aatgcgtttg tcgtcgagca tccgctcaac cagcacttca atcttgccgc
    12121 cgctggcttt acgcccaaac aggcgcgccg ggatcacgcg ggtattatta aaaaccagaa
    12181 gatcgccggg gttgagctta tcgagtaaat cggtgaaagt accgtgcgtc agcgcgcccg
    12241 tcggcccgtc cagcgacagt aaacgacagc tactgcgttc aggcatcgga tagtgggcaa
    12301 tcagggattc gggcaattca aaggagaaat cggtaacgcg catgacactg actcagacta
    12361 aaataagagg cgggtagtct agtgccgggg cgctctccct gcaaccttta cccccctttg
    12421 gatagaatat aatcgatgaa ttttttagct cacctgcatt tagcccatct cgcggaaagc
    12481 tcgctttccg gcaatttgct ggctgatttc gtacgcggaa atcctgaaga aagttttccg
    12541 cccgacgtcg tggctggcat tcatatgcat cgacgtatcg acgtattgac tgacaatctg
    12601 ccggaagtcc gcgaagcacg ggagtggttt cgtagtgaaa ctcgccgcgt tgcgcctatt
    12661 acgctggatg tcatgtggga tcactttctt tcccgtcact ggtcgcagct gtcgccggac
    12721 tttccgctac aggaatttgt ctgttatgcc cgcgaacaag tgataacgat tttgccggac
    12781 tcaccgccac gctttatcaa cctgaacaac tacttatggt cagagcagtg gctggtgcgc
    12841 tatcgcgata tggatttcat ccagaacgtg ttaaatggca tggcaagccg tcgcccacgt
    12901 ctggatgccc tgcgtgactc ctggtacgat ttaaacgccc attatgacgc cctcgaaacc
    12961 cgcttctggc agttttatcc gcggatgatg gcgcaggcgt cacgcaaggc gctctgaaac
    13021 gtcaaactcc ggcgtcacta ctttttagca ttcgcatcca gcggggcggc ataacaacgg
    13081 taagaaaaac attcgctgga atgattggcg atatctttaa agttgctttc agcaaccccc
    13141 tcgaacatga gtaccgttct ggacaactct ccgttgtcat ttatatgtgg aaaatcaaat
    13201 tgtgtactga atcgccatac atctgtcttt tctggctcgc tgttcacaga gatacgtcga
    13261 tggaagactc ctcctgaaaa cgtgcgccag aacaattgtt gatcaaaatc ttgtaatatc
    13321 gcacacttgg atgctatttg ttgtagtaaa cctgcatacc gtggactgcc atcacgagca
    13381 acattataca aacactgact aaaagcatcg aacaaacaat agatgtgatc actagtgaaa
    13441 aaaggtctta attttttttg ttcttcgtcg ctgtaatcca ggagcatctc attgatttct
    13501 tcctgtgaga attttaagac aaaattatct tcaggatttg tttctttgtt gtttttttca
    13561 atattcttct cctcaatgct attcgcattt tgtttcgcaa ttatttccga attttttttc
    13621 gcagtttttt ccgcagtttt ttccgcagtt tttttcgcaa tttcttctgc aattttttcc
    13681 cgagtttttg attttagact aacaccatcg atgtcaaccg gtgttgtttt cagatctctc
    13741 tgcgcgtctt tcaaatttgt cttatagaga tcgggcactt tatcggcaga taggtatccg
    13801 gatgaagaaa tctggtgtgt acacatgggg ggctgttcac aaagcatcga ggttatatcg
    13861 tctttaaact cttttacatt aaacttctcg gcattaacct tccctgttat aaaccccgct
    13921 ttggcaattt tcttctctaa ttgttttgta cgtttagaat aataagcgtt gttatcataa
    13981 aacttttcaa gaagggccca ctcctcaagt tgccttatgt gatttttcaa ctgttgatga
    14041 tctattttat attcatgtgt actagaatca ttttggttgc aggagttaca gatttttaag
    14101 cataattttc catcagattt atcatctatg gtatcagtga aaatatagga cacttcaata
    14161 ttctgcccgt tcttatcagt catctcatat gaccattgcc ccgttcgttt cgcattctta
    14221 atgagttcga tggactctgc aggacaacca tgaagaatgg gcagaatttt atcaggatac
    14281 ttaatgagta aaattattat atttacaacg tttgaaaaga aatttttgct attttttatt
    14341 tcatcgattt tttccgctga aatgtttact aagttttcat ttccgcaaat gaaaagcgat
    14401 ttgccatggg tgcttttttt aaattgttct tctgttttag aaaagacatc ctccttaaaa
    14461 ggtggcaact cagcaccagt aataaacggt ttcaataaat tcgaatttga aattgtcata
    14521 tctttgctcc actacagggt tatcctggag catgttgtac ataacatcat gggcaaagaa
    14581 taccaaaaat cgctcatatc gatcgggaca aatatttaac aaattcagcc ctgacattga
    14641 gccgcttttt tgcgctgcgt agtgtcaggg ctggtgacta acgacgtaac aaataaaaca
    14701 gatgctttag ttcatccaca ccgtagccga aatggcaggc aaagccagaa tcccgtctgt
    14761 cagttgccca tggccttctt tgcgttgcca ttgcgcgaca ttaagaaacg gtgacgcggg
    14821 gagcaccact tcacaggcct cgccacggtt gattgccacc agtacacgct gctgatttag
    14881 cacgcggaca aataccacca cgttatcttc cgcatacagt acctgacagc cgccacgacg
    14941 cagcgcctga cttttcttac gcagcacaat cattcgctgg tacagcgcga ataacgccgt
    15001 atcctgcttt tccacctgcc aggggaacgg tttacggcaa aacggatcgt ttttaccatc
    15061 cagtcccact tcatcaccgt aataaatgca cggtacacca ggccaggtga acagccagac
    15121 caccgccagg ggcaggcgcg caacatcccg accgagcagc gttttaaatc gcgcagtatc
    15181 gtggctgtcg agctgattaa acatacgtaa ttgctgttga tgagaaagcc ctgcgcggta
    15241 attatccatc caggccatac aggtttgggc gtcaatttgc tgcggatcgt aagagatgtc
    15301 ggtattggca agaaatcccc acaacgggaa tgtgaagccg cgatagttca tggcggcatc
    15361 ttccacatcg gcctgtaacc attgccgtgc atcgccaaaa tgctctccaa ctatatacgc
    15421 ttccggctgg gtttctttcg ccgcttcggt gatcccagca acgtgctgca aattattgcg
    15481 cgccccaccc gcttccccca gcatatgcac cacatccagc cgccagccgt ccatattcca
    15541 cggcgctttc agccagtggc ggacaatact gtcttccccg cgataaattt cattcaccag
    15601 actttccgac tgataatcca gcttcggcaa gctggcatag ccaagccagt cgagcgccgt
    15661 gccatcatca ctaaacgagt accagtcgcg ccaaggcgat tcggggttgt gacaagctcc
    15721 ccccgtgcca cgattgtgcc tgtcaaacca ggcatgggaa tcgccactgt ggttaaacac
    15781 gccgtccagc accagacgca ttcccagctg ctgcgtattg tgtcgcaaac gcagcaacgc
    15841 cccatcaccg ccaaactgcg gatcgacatg gcgataatcc tcggtatcgt atttatgtac
    15901 gctgggagct ttaaacaccg gattgagata caacgccgtc acgccaagtt ttttcagata
    15961 cggcagtttt tcgcttatcc cgtccagatc gccgccatag aacgttgatc cgcccgcctg
    16021 cgccgtgacc ggttcatccc agtcacgcaa tatgatctct tgtccggctg cgtgatggta
    16081 atagacatga tcctgttcag cttcacgagg aagactacgg gcgaaacgat cagggaagat
    16141 ctgataaaaa atctgatccg cagcccattg tgaaccgata tccggtacat cgatggcaaa
    16201 ctgctccagt cgtgctggcg gcattcggct gaagccttgc ggtgtaaacc aacgctggcg
    16261 atcgtgccaa agcaatttga aactataacg ccgccggggt tgtccgctgg agagatcgat
    16321 cgccgcccgc catgccgtga cgcctggctg cggctgactg cgctgcttat gcatcggtac
    16381 tgacatttct tcgttatcgt gttctgtacg cagcataatg cgctgcggtg ggtcttcgcc
    16441 cgtcagccac agtgtaatga gcagttgatc tttgctttgt ttaacaaatg ggggcaccgg
    16501 caggtgccat gcatttaaca tcataattcc cctgtgatga aaatctgatc accttgccat
    16561 agcctaatgc aggatcactc atcatctgaa ggatatttgg gggaggagga cgggggcgga
    16621 taactttcgg aatagccgga tcaggatttc ctcctgaacc ggcttacaga acgcaagtat
    16681 tactggtttt cagccagctg acgatcgtgg cggcgtttaa acatccagcc aatcaacagc
    16741 acaacaatcc acgcgaaacc gacatacagc gagatacgcg tatccgggtg ataaccaatc
    16801 aacccgataa taaagagcag gaaaatcagc ccgccgatgg tcgttgctac cccacccggc
    16861 actttaaatt tcagcgcctt aacttcttct ggcggcaaac ggcgacggaa ggcgatttgc
    16921 gacagcagga tcataatcca cacccatacc gtggcgaagg ttgccagcga agcgatcacc
    16981 aggaagacgt tttccggcat gatgtagttc agatacaccg caaacagcag cgcggtagtc
    17041 atcaccagca ccgtaaccca cggaataccg cgacgcgacg ttttgctgaa aattttcggt
    17101 gcgctgccct gctctgccat accgtggagc atacggccta cgccaaatac gtcactgtta
    17161 attgccgaca gcgaagcagt cagcacaaca aagttaagaa tgctggcggc aaaggtaatg
    17221 cccatatgct ggaacgtcag cacgaacggg ctaccggcag tgccaacctg attccacggg
    17281 tagatagaca taatgacgaa cagtgtacca acatagaaca ccagaatacg catcggcacg
    17341 gagttaatcg cacgcggaat agatttctca ggatctttcg cttcaccggc ggtaatcccg
    17401 ataatttcga tcccaccgta agcaaacatc accatttgca acgacattac catgccaagc
    17461 cagccgttac tgaagaagcc gccgttgctc cacaggttat gaataccggt cggttgcccg
    17521 ccgttgccaa tcccccagat gatgatgccg aaaccggcga caatcatgat gatgatggtg
    17581 gcgactttaa agaacgagaa ccagaattcc agctcaccga ataccttcac gctcatcagg
    17641 tttacggcgc aaatgatcag caccacgctc agtacccaaa tccagtgcgg caccgtcggg
    17701 aaccagacac ccatatagat accaaaagcg gtcacatcgg cgatggcgac aataaggatt
    17761 tcaaagcagt aggtccaacc ggtaatgtaa cctgccagcg ggccgaggtt ttcctgcgca
    17821 taacgcgaga aagagctggc ggccgggtta tgtaccgaca tttcccccag cgcacgcata
    17881 atgatatacg ccgcgatacc accgataata taggccaaca acacgctcgg accggccatt
    17941 ttgatggcgt ctgccgaacc gtaaaacagc ccggtgccaa ttgctgaacc cagtgccata
    18001 aagcgtatgt gtcgggtact tagcccacgc tttagcttgt tcttactttc catcaattcc
    18061 taacccatca acacaataaa aaaccacggg gcatcgcagc cccgtggtta aaacaaatgt
    18121 tcagtgattt agtgagcgct ggaggtcacc tgacgacctg ccgcgcgatc ccagataatg
    18181 gccagaacca ccatcaccac tgttggcatt aaccacgcca gaccttgttc ggccagcggt
    18241 aaacgctgcg cccaggacgg taagatatcg ctgaatgcag atgctttgat cccgtcgaga
    18301 ataccaaaaa gcaggctgat aaacatcggc ggagcaatca cgcgggacga attatgccac
    18361 catgagcgtg taaaacttaa tacaaccagt gcgatacacg gcggataaat agcggtcagc
    18421 accggtacgg agatctggat cagctggctt aagccgaggt tagaaaccac catcgagaag
    18481 ccgccgagga taaacaccag cgtacgataa gagagcggta cgtactgggc aaagaattct
    18541 gcacaagcac aggtcaggcc aactgccgtt accaggcagg cgatgaagat taacgccgcc
    18601 agcaggaagc taccgccgcc gccaaaggtg tgctgaacgt aagcatgcag aatagcagcg
    18661 ccgtttgcag actgatcgac cagcgacgcg ctgtctgaac ccagacggaa cagcgccagg
    18721 tacagcagag tcagaccaac acccgccatc aggccagccc agacggtata acgggtcagc
    18781 agacgcgctt cagtaacgcc acgagaacgc gccgcgttaa caataacgat accaaacacc
    18841 attgcgccca gcgtatccat ggtcagatag ccgttaacga agccgttaga aaacgcagcg
    18901 ttttgataag cctcagtcgc cgtgctgata gaacccgccg gccagacaat cgcggcaaca
    18961 gacaggatga ccagcgcgat aattttcagc ggtgcaagga agttgcccac ggtatccagc
    19021 agcttgcccg gatagagcga aaccagaata acgatagcga aatagaccag gctgtagata
    19081 aacagcggca gtgcggaatc acccgtcagc ggcgcaatcc ccacttcaaa agaaacggta
    19141 gctgtacgcg gcgtagcgaa aagcggcccc accgccaggt aacacaccgt tgccagcagt
    19201 acgccagcga ctttaccgat tggcgtgctg aggctgtcaa caccgccgcc aacttttgcc
    19261 agcgccacta ccgttaatac cggcaggcca acggcagtaa tgaggaagcc gaatgccgca
    19321 gtccagacgt gttcgcctgc ctgcaagccg accattggag ggaaaataat gttacctgcg
    19381 ccgacgaaca acgcaaatgt cataaagccc agagcgatga tatcgcgcga tcttaattga
    19441 tgggtcataa atcatactgc ctgtggatgt ggtgttgtaa cgttaaattt ttgtgccaat
    19501 ccctggggat gatacagcgg tatatttaaa caattttagc gggaaatact cccaaccagg
    19561 cgcggcgaag aatagttttt cgatttaccg cgttaatgca gtcagtatga cagcacctga
    19621 agcgcaattt aaacgcttat aacgttttaa agcaaggagg gatctaaaaa ccagaggtat
    19681 atagcgataa gaaatcacca accagtataa aacactattt ccgtgaaaaa gacatggcta
    19741 atcatgcgaa caaaaaatca ccaaccgttg taaaatctaa cgcccgtatt ttgactgcaa
    19801 agaaaataag acaacatcgg ctggcttatg gcaataaaag atgacaaagg cggattaatc
    19861 gctgtttttg gcaattaaac gttccgggat aacaaagctg aaacgtgttc cttttcctac
    19921 tgtactctca atattcaggc gactttcgtg atgattcacc gcatgtttca cgatcgctaa
    19981 ccctaatccg ctaccgccgg tttgccggga acgcgcttta tcaacgcgat aaaaacgctc
    20041 ggtcaggcgc ggaatatgct ccggtgcaat gcccggtccg ttatcttcaa cgctaaattc
    20101 ggcaccgtgc ggcactcgct gccagcgtac ggtgatatgc gtgccttccg gcgtatgatt
    20161 cacggcgtta tagaccaggt tcgaaatcgc actgcgtagc tgatcttcgt tgccagacac
    20221 cttgaggccg ttatctatct caaaggtaaa tgtctgtttt ttctgactca gagtctgagc
    20281 ctcgcgctca acaacgcgca gcatcatcgg cacatcaacc ttttcattga gcaaatgcgt
    20341 cggtgcggct tctattttcg acagcgtcag caattgcttc accaaccctt ccatccgctg
    20401 ggtctgctcg cgcatggtgt gcaacgcttt ttcgcgtacc gcgccttcca gcggctgctc
    20461 atccatcatc tccaggtaac cctgtaacac ggtcaatggc gtgcgtaact catggcttac
    20521 gttggcgaaa aagttgcgcc gcgccccttc cagttgatgc atttgcgtga catcacgcgc
    20581 caccatcagc aactgtttgt gggtataagg catgacgcga atttccagat gccgcccggt
    20641 gttgagcacc agattgagcg ggcgagaaaa atcacgcgtt ttcagatatt gcgtaaactc
    20701 cgggtaacgc agcaggttaa ggatgttctg cccgttatct tccggccagc gcaaaccaag
    20761 aatctgttgc gccagaccgt tacaccagaa aataccgccc tcttccgtgg tcagcaccac
    20821 tgcgtcgggc agcgactccg cgccgctacg aaagcgttta atcagattgc ccagttcacg
    20881 gcggcgtttt ttatttcgca gctgcatctg gtgtaagccg tatagtagcg gttcccagct
    20941 accacgcccc ggtggcgggg tcatactacg atccacccac agccaccatg aaaggcgcaa
    21001 taaattccag aaatgccaga taagcagtcc cgttaccgat gccagcaaaa accagggcag
    21061 gtaaccaaaa aatgcaccca ggatgaaagc cgggaggcag caaagtagca gctccagcac
    21121 cagccttttc cacgacagcc gttccagcac gtaagatact ccagttaaga aatcataagc
    21181 cctgctctgc gtccgatgag caaggcgtta aaagcgggtt gaaaaacgat atcctgtacc
    21241 gcgcacggtc tgcaccatgc ggtcatgccc gccgggctcc agtgctttac gcaggcgacg
    21301 aatgtggaca tcgaccgtgc ggtcttccac atacacgtta gttccccaga cgtggtttaa
    21361 cagctgctcg cggctgtaca cgcgttcagg atgcgtcata aaaaagtgca gcagtttaaa
    21421 ttctgtcggc cccatctcca gcggctcttc gcccgccatc acccggtgag atgtcgggtc
    21481 gagacttaat ccctgcatct caatcacctc ttccaccgcc attggcgaaa tacggcgcat
    21541 taccgctttg attcgcgcca ccagctcctt cggcgaaaac ggcttggtga tatagtcatc
    21601 cgcgccggtt tcaaggccgc gcacgcgatc ttcttcttcc cctctggcgg tcaacatcac
    21661 cactggaata tcccgggtca tcgactcgcg cttgaggtgt ttgatgaact ggataccgga
    21721 gccgccaggt aacatccagt caaggagaat taaatccggc cagggttcat tcagttgatt
    21781 cacagcactg tcataatctt ccgcttcgac cggctgaaag ccattttgtt cgagcacgaa
    21841 gcagaccatt tcgcgaattg gagcttcatc ttctacgacc agaatacgtc tcgccatgat
    21901 ttgccctgtt gtaataaata ggttgcgatc attaatgcga cgtcattatg cgtcagattt
    21961 atgacagatt tatgaaaagc tcgtcgcaca tctcttcagg ttattgattt ccgtggcgca
    22021 gaaaaaagca aatggcacat ctgtttgggt ataatcgcgc ccatgctttt tcgccaggga
    22081 accgttatgc gcattcttca cacctcagac tggcatctcg gccagaactt ctacagtaaa
    22141 agccgcgaag ctgaacatca ggcttttctt gactggctgc tggaaacagc acaaacccat
    22201 caggtggatg cgattattgt tgctggtgat gttttcgata ccggctcgcc gcccagttac
    22261 gcccgcacgt tatacaaccg ttttgttgtc aatttacagc aaactggctg tcatctggtg
    22321 gtactggcag gaaaccatga ctcggtcgcc acgctgaatg aatcgcgcga tatcatggcg
    22381 ttcctcaata cgaccgtggt cgccagcgcc ggacatgcgc cgcaaatctt gcctcgtcgc
    22441 gacgggacgc caggcgccgt gctgtgcccc attccgtttt tacgtccacg tgacattatt
    22501 accagccagg cggggcttaa cggtattgaa aaacagcagc atttactggc agcgattacc
    22561 gattattacc aacaacacta tgccgatgcc tgcaaactgc gcggcgatca gcctctgccc
    22621 atcatcgcca cgggacattt aacgaccgtg ggggccagta aaagtgacgc cgtgcgtgac
    22681 atttatattg gcacgctgga cgcgtttccg gcacaaaact ttccaccagc cgactacatc
    22741 gcgctcgggc atattcaccg cgcacagatt attggcggca tggaacatgt tcgctattgc
    22801 ggctccccca ttccactgag ttttgatgaa tgcggaaaga gtaaatatgt ccatctggtg
    22861 acattttcaa acggcaaatt agagagcgtg gaaaacctga acgtaccggt aacgcaaccc
    22921 atggcggttc tgaaaggcga tctggcgtcg attaccgcac agctggaaca gtggcgcgat
    22981 gtatcacagg agccgcctgt ctggctggat atcgaaatca ctactgatga gtatctgcat
    23041 gatattcagc gcaaaatcca ggcattaacc gaatcattgc ctgttgaagt attgctggta
    23101 cgccggagtc gtgaacagcg cgagcgtgtg ttagccagcc aacagcgtga aaccctcagc
    23161 gaactcagcg tcgaagaggt gttcaatcgc cgtctggcac tggaagaact ggaggaatcg
    23221 cagcagcaac gtctgcagca tcttttcacc acgacgttgc ataccctcgc cggagaacac
    23281 gaagcatgaa aattctcagc ctgcgcctga aaaacctgaa ctcattaaaa ggcgaatgga
    23341 agattgattt cacccgcgag ccgttcgcca gcaacgggct gtttgccatt accggcccaa
    23401 ccggtgcggg gaaaaccacc ctgctggacg ccatttgtct ggcgctgtat cacgaaactc
    23461 cacgcctctc taacgtttca caatcgcaaa atgatctcat gacccgcgat accgccgaat
    23521 gtctggcgga ggtggagttt gaagtgaaag gtgaagcgta tcgcgcgttc tggagccaga
    23581 atcgggcgcg taaccagccg gacggtaatt tgcaggtgcc acgcgtagag ctggcgcgct
    23641 gcgccgatgg caaaattctc gccgacaaag tgaaagataa gctggaactg acggcgacgt
    23701 taaccggact ggattacggg cgcttcaccc gttcgatgct gctttcgcag gggcaatttg
    23761 ctgccttcct gaatgccaaa cccaaagaac gcgcggaatt gctcgaggag ttaactggca
    23821 ctgaaatcta cgggcaaatc tcggcgatgg tttttgagca gcacaaatcg gcccgcactg
    23881 agctggagaa gctgcaagcg caggccagcg gcgtcgcgtt gctcacgcca gaacaagtgc
    23941 aatcgctgac agcgagtttg caggtactta ctgacgaaga aaaacagtta cttaccgcgc
    24001 agcagcaaga acaacaatca ctaaactggt taacgcgtct ggacgaattg cagcaagaag
    24061 ccagccgccg tcaacaggcc ttgcaacagg cgttagccga agaagaaaaa gcgcaacctc
    24121 aactggcggc gctcagtctg gcacaaccgg cacgaaatct tcgtccgcac tgggaacgca
    24181 tcgcagaaca cagcgcggcg ctggcgcata ctcgccagca gattgaagaa gtaaatactc
    24241 gcttacagag cacaatggcg cttcgcgcga gcattcgcca ccacgcggcg aagcagtcag
    24301 cagaattaca gcagcagcaa caaagcctga atacctggtt acaggaacac gaccgcttcc
    24361 gtcagtggaa caacgaactg gcgggttggc gtgcgcagtt ctcccaacaa accagcgatc
    24421 gcgagcatct gcggcaatgg cagcaacagt taacccatgc tgagcaaaaa cttaatacgc
    24481 ttgcggcgat cacgttgacg ttaaccgccg atgaagttgc tagcgccctg gcgcaacatg
    24541 ccgagcaacg cccactgcgt cagcgcctgg tcgcgctgca tgggcagatt gttccccaac
    24601 aaaaacgtct ggcgcagtta atggtcacta tccagaatgt cactctggaa cagacgcaac
    24661 gtaatgccgc acttaacgaa atgcgccagc gttataaaga aaagacgcag caacttgccg
    24721 atgtcaaaac catttgcgag caggaagcgc gcatcaaaac gctggaagct caacgtgcac
    24781 agttacaggc gggtcagcct tgcccacttt gtggttccac cagccacccg gcggtcgagg
    24841 cgtatcaggc gctggagcct ggtgttaatc agtctcgatt actggcgctg gaaaacgaag
    24901 ttaaaaagct cggtgaagaa ggtgcgacgc tacgtgggca actggacgcc ttaacaaagc
    24961 agcttcagcg tgatgaaaac gaagcgcaaa gcctccgaca agatgagcaa gcacttactc
    25021 aacaatggca agccgtcacg accagcctca atatcacctt gcagccacag gacgatattc
    25081 aaccgtggct ggatgcacaa gatgagcacg aacgccagct gcggttactc agccaacgac
    25141 atgaattaca agggcagatt gccgcgcata atcagcaaat tatccagtat caacagcaaa
    25201 ttgaacaacg ccagcagcag ctcttaacgg cattggcggg ttatgcactg acagtgccac
    25261 aggaagatga agaagagagc tggttggcga cacgtcagca agaagcgcag agctggcagc
    25321 aacaccagaa cgaattaacc gcgctgcaaa accgtattca gcagctgacg ccgattctgg
    25381 aaacgttgcc gcaaagtgat gaactcccgc actgcgaaga aactgtggta ttggaaaact
    25441 ggcggcaggt acatgaacaa tgtctcgcat tacacagcca gcagcagacg ttacagcaac
    25501 aggatgttct ggcggcgcaa agtctgcaaa aagcccaggc gcagtttgac accgcgctac
    25561 aggccagcgt ctttgacgat cagcaggcgt tccttgcggc gctagtggat gaacaaacac
    25621 taacgcagct ggaacagctc aagcagaatc tggaaaacca gcgccgtcag gcgcaaacgc
    25681 tggtcactca gacagcagaa acgctggcac agcatcagca acaccgacct ggcgggttgg
    25741 ctctcactgt gacggtggag cagattcagc aagagttagc gcaaactcac caaaaattgc
    25801 gtgagaacac cacgagtcaa ggcgagattc gccagcagct gaagcaggat gcagataacc
    25861 gtcagcaaca acaaacctta atgcagcaaa ttgctcaaat gacgcagcag gttgaggact
    25921 ggggatatct gaattcgcta ataggttcca aagagggcga taaattccgc aaatttgccc
    25981 aggggctgac gctggataat ttagtccatc tcgctaatca gcaacttacc cggctgcacg
    26041 ggcgctatct gttacagcgc aaagccagcg aggcgctgga agtcgaggtt gttgatacct
    26101 ggcaggcaga tgcggtacgc gatacccgta ccctttccgg cggcgaaagt ttcctcgtca
    26161 gtctggcgct ggcactggcg ctttcagatc tggtcagcca taaaacacgt attgactcgc
    26221 tgttccttga tgaaggtttt ggcacgctgg atagcgaaac gctggatacc gcccttgatg
    26281 cgctggatgc cctgaacgcc agtggcaaaa ccatcggtgt gattagccac gtagaagcga
    26341 tgaaagagcg tattccggtg cagatcaaag tgaaaaagat caatggactg ggctacagca
    26401 aactggaaag tacgtttgca gtgaaataac tattcagcag gataatgaat acagaggggc
    26461 gaattatctc ttggccttgc tggtcgttat cctgcaagct atcactttat tggctacggt
    26521 gattggtagc cgttctggtg gttgtgatgg tggtatgaaa aaagtcattt tatctttggc
    26581 tctgggcacg tttggtttgg ggatggccga atttggcatt atgggcgtgc tcacggagct
    26641 ggcgcataac gtaggaattt cgattcctgc tgccgggcat atgatctcgt attatgcgct
    26701 gggggtggtg gtcggtgcgc caataatcgc actcttttcc agccgctact cactcaaaca
    26761 tatcttgttg tttctggtgg cgttgtgcgt cattggcaac gccatgttca cgctctcttc
    26821 gtcttacctg atgcttgcca ttggtcgcct ggtttccggc tttccgcatg gcgcattttt
    26881 tggcgtcgga gcgatcgtgt tatcaaaaat tatcaaaccc ggaaaagtca ccgccgccgt
    26941 ggcggggatg gtttccggga tgacagtcgc caatttgctg ggcattccgc tgggaacgta
    27001 tttaagtcag gaatttagct ggcgttacac ctttttattg atcgctgttt ttaatattgc
    27061 ggtgatggca tcggtctatt tttgggtgcc ggatattcgc gacgaggcga aaggaaagct
    27121 gcgcgaacaa tttcactttt tgcgcagccc ggccccgtgg ttaattttcg ccgccaccat
    27181 gtttggcaac gcaggtgtgt ttgcctggtt cagctacgta aagccataca tgatgtttat
    27241 ttccggtttt tcggaaacgg cgatgacctt tattatgatg ttagttgggc tagggatggt
    27301 gctgggaaat atgctaagtg gcaggatttc aggacgttat tcaccactgc gcattgcagc
    27361 agtgactgac tttataattg tactggcact gctgatgctc tttttctgcg gcggtatgaa
    27421 aacaacgtcg cttatttttg cttttatttg ttgcgcggga ttatttgccc tttcagcacc
    27481 tctgcaaata ttgttactgc aaaacgccaa aggcggagag ttattaggtg ccgcaggtgg
    27541 gcaaatagcc tttaacctcg gtagcgccgt cggcgcatat tgcggtggta tgatgctgac
    27601 gctggggctg gcatataatt acgtggcgct gcctgccgcc ctgctttcgt ttgctgcgat
    27661 gtcgtcattg ctgctgtatg gtcgctataa gcgccagcaa gcggcggata ctccggtgct
    27721 ggcgaaacct ctggggtaga ttgcagtcac ggagcacgtt gcttattgcc ggatgcggcg
    27781 tgaacgcctt acgcggttct ggcacatttt gtaggcctga taagacgcgg caagcgtcgc
    27841 atcaggcatg atgcgccaat tgcctgggtt ttttactctt gtggccataa ccacgcagcg
    27901 ccgcgcacgc cgctggaatc accatgcttc gccttacgca ccggcgtttc acattcgccg
    27961 ccgaagacaa attgtttaat caactgcgga accgtttgat ataaacggtc tacattgctc
    28021 atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg tgccagcgat
    28081 tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc gctttcttca
    28141 accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg ataatccgtc
    28201 gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca ataacaaggg
    28261 acttcctcgc gataacgcag ttcgtcttcg tccatccacg gtagcggatt gtgtccccac
    28321 tcacctgccg tgccattgcc gccgatatgc gcccgcccat tgaatgccac gcccgcgccg
    28381 catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc gccatctact
    28441 gcttctgaaa ccgccagaca gttggcgtca tttgccagcc gcacttcccg ctgcaacctc
    28501 gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc attcttcacc
    28561 acaccggtgt aaggcgaaat tgagccagga atgcccatac ctaccgttcc gcgctgcccc
    28621 gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg ccggtaatca
    28681 tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc cagtgcaatc
    28741 acttcagttt tggtgccgcc taaatcgata cctatacgca cggtactctc cttatttttt
    28801 tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg ccgacaattc
    28861 gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct gtggttcaag
    28921 aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga gatggaaaaa
    28981 cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa gatgggctgg
    29041 gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg tcaaattgtt
    29101 atctgcgcac gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca ggcgctggaa
    29161 gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac cgaaaaagat
    29221 tcgctgaaag acgaagtgct gcactctctg ttgccgcgtg ctttcagccg ttttagccag
    29281 acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc cagtgccaaa
    29341 aaagcggaag atacgctggc actgctgcgt aaaagcctgg ggtcgttacc ggttgtacca
    29401 ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggtacgttc cggtagtgcg
    29461 gcacagggct tccagctgct tgatgaagcc gagctgaaat cgttgctgga agatggcggc
    29521 gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca cattgaagcc
    29581 ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt tgtgatgtgc
    29641 gacgatggtt cgctcaagcg tctgaagttc tgcgacgagc tgcgcgatca aaacgaagat
    29701 atcgaccgtg aagatttcgc ccagcgtttt gatgccgatt tcatcctgat gactggtgaa
    29761 ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca acgttaattg
    29821 ctgatttacc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg tacgaagcca
    29881 caccaggcat ataattattc gctacggcgg gcaataattt ttagcgcagc aatattatgc
    29941 gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc agcacgcaga
    30001 atattcacta acccccattt atcgctggta aattgttttg ttaacagctc cgcttttggg
    30061 gtgtcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag gtaaggatac
    30121 cagcaagttt caccgtgaaa attacgcact ccaccgttac ctgcaataaa gtctttcggg
    30181 taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc ggtgcttcca
    30241 ccattttggc tgcgcatggg ataacgggta cagtgaattt gcaggttgat cgttggcgtc
    30301 agtaagtcat agatctcccg ctgtaacttg ctccatgggc gctgtgtcat tgtttttcct
    30361 caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc actaaatcac
    30421 gtaacagcga aagatcgggt tgttcgacaa ataagagtaa tgatggatcc tcttttaacc
    30481 acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac cgatcctgag
    30541 gctcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat gccgtttggc
    30601 gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct gatttatctt
    30661 ttatcgtcgc cagttccgca aaaatatcgc tatcctcatg gttccagcgg tgtaatcctc
    30721 tttcactttg tctacgggtg ctatttttca tactgacaac agccagcgcc cagtcagagt
    30781 tgaagcgata ctgttgatcg agtttgttga taattccgga aggtaattct ttattatcaa
    30841 ataattggtt acgcattcga tagcggtatt cagcgggtaa atcaaccagt aaatcaatgg
    30901 cattatttaa catctcttcg tcaacaacac cattatcaat cagcgccagc tgtacttcgt
    30961 aggcttgctc ttgatcttct tgccgtttgc tcatcattaa atttaatgtg cgttgccata
    31021 actgtttctt atattccagc ggcaataatt tatcgcgtgc cagctcatcc cagacggtgg
    31081 gaacatcatg ctgagtcagt agataactaa tcacttcctt cgatgtcaga taacgcgtac
    31141 cttcgcgtaa attgtgcaca tcttcttttg ccagagacag ttgacgcatc tccggcaacg
    31201 ctatcaataa cgcttttacg atatttttat tttctttatt atccagatac atttgttctg
    31261 cgatacgttc aatatcctct gtacgcaggg ttgctgacag ttgggtaatt tttaattcag
    31321 taagcgtccg ggctaatgct tcacgaactt tcagtgattt atctttactc aacagcacgg
    31381 cttgttgtgc ggttaaatct gctccggtaa ttctttcgcg taactccgga tcttcgctat
    31441 ttatcaactc atcggccact gctgtcggaa ccagccaggc gtaatcgtaa tggctgagta
    31501 atgtcttgcg tacattgaga aaggggctat gacttaacga cgcaacaata tctggagaaa
    31561 gattatcctg tctggcctgc tccagctgta cgctctcgac gggtacagac attaattttt
    31621 tattcacatc ggcggaaata ttactgtttt taaccagccc tctggcaaca gcctcatctt
    31681 taattaattc atcggtaagc aaatgcatct gttgagcggg ccaggactgc gccatcgatt
    31741 cacgcgtctg gtcatcgggg tgctgtaaca gttgcagcat aacggcgggt gataaactct
    31801 ctgtatgagt cagaaaacgt gccaccagtg gatcttgatg ctttatttta ttcgctggga
    31861 aattatcgag tttccccgtt tcaatccatt ttaatttctc attaatttca ggagcggctt
    31921 tatctcgaaa aagcggtaaa ccctgaacga tgttgtctgc gacgctatca tagtcaggat
    31981 acgcattgga aatgttatcg gtttcgattt cagtgtgatc ataaagatcg gcagtcacca
    32041 gtgaataggc tttttgaata ttctcatccc agttatatcc ttcaccactt ccatcttcat
    32101 tgatataaaa ttcagcttta atgacttcaa attgcgttgc tttatcggaa acaataatgc
    32161 ctcgcgcact aattcccttc atcacttcaa agttatgatc attgtaatca gcataaacta
    32221 aacctttcgc tgtcatctgt cctttgacat acgcgaaatc atgattaata aagttatcga
    32281 caataacatt acctaataca atcagatgac cattacctga cagataatca tcataatcgc
    32341 cggaaatcgt taaattaccg ttcaccacca gtttattttg attgctgaag ttagcgggta
    32401 atgataggtt accattatga taataccagc ggatattatc ttttagcata taagcagctt
    32461 ccatactatc gtcggttgcc gctttaacca cgttgcgctc atctgccgtc ataagatgaa
    32521 agtcagaggt gataaggtcc tcgctactga tccactctgt ggcacatgca tagccagtca
    32581 taaaaaataa gaccatacag atgaccaata tattaattct attcataatc ttcaatgata
    32641 tttatatcaa ccttgatgaa ttatgaatat atcggaaatt gaaaaaatcg cagcaaagga
    32701 aatattatta tgggttaaag agaggatatt tcagaaaaat gccctcccgt tcaaggggaa
    32761 ggcgaggaat tacagatagc ggcacagata agaggtgggt tcggcaactt gcagatgaaa
    32821 ctcactgtga ccgggaacat taaacaccga accggcttca tacacttgcc agtcggtcgc
    32881 gtcaggcagt aacacattca gcgcgccact gattaccgtc atctcttccg gctcagcggt
    32941 gctgaaggtg tattcgcctt caaccataac acccacgctg gcgcgaccag tgctgctgct
    33001 ggaaaagccg atcgatttca ctttgccgga aaagtactca ttactttgaa gcataaactg
    33061 gccctgtctg gtagttgaac atctacttca ctataggggc cagaggcgct ggctgtcacg
    33121 caaaattaca ccagtaattc cgcagccaac cgtgcaatca atacgttaga gagcaagaca
    33181 ggaacatcga gctgtttttg cagtaaatcg cgatgacgct ggttaaatcc caaacaatcc
    33241 agcatgatga catctgcgcc tttcgccagt aattctttcc cggcatcaat gattttttgt
    33301 tctgaatcat gaatggggtt acccaatgaa aataccggcg gtttctgcaa aatttgccat
    33361 ttttgcgcct gaacggtcag caactcctca accggtacga taacccccac ctgatgatct
    33421 tcaacaatag aggaaaccag cggtggcaat atccgcgacg gctcaagaaa gatcgtatta
    33481 cgcgcagtca tactactaat gtttgccgta ctcattaata taatgacgtc gtagccctga
    33541 ttatcgagca cttcaaccac accttgcagg tcacgctcta ctttgcgacg cgaaacatgg
    33601 gccagatggt tgtcatttaa taatgtgaga attgtgtctt cgcctgcttc tggcgcgtac
    33661 tccgccatca cttcttcgcg gcttaacttc cccagcaggc tatgatggga aatattatct
    33721 tcgtcaatgt attctgtcag gagcggcaaa acttcctgca taggtacaat gccgatggtt
    33781 agaatcgcca acgacgcact cattgttctt ccaccttact tttatttcgt cgttaattac
    33841 cgggcaagtg ttaagcacca ctgctgacac ttacttgtgc tacaaatgac aaaaagcgta
    33901 gcagcagacg tgcacggcat aatgtaaaga ttccaaatga ttccagtaat ggattgttat
    33961 tgtttaatat tctaattatt tgaaaaacat gaattatgaa aaatgtgacg cagatccctt
    34021 gtagtggcac tcagaatccc ttccgagcag tctgatgcgg tcactctttg tcagggtcct
    34081 catagcgttt tttcgcatcc atcgcttcct gagcggtagg atgttcactg atcaacgagt
    34141 ctggtttagg atgatcggct ctgagttggt accaggttac cgtctgtcct ggttttcctt
    34201 tttcaatcgt cactatatat gcttcacgag gataaggcgg tttcgttggc atcgttcttc
    34261 cttatttcac gggatgaacg ttaagtatag gcgctctaaa atcaacaatt gatcgtctgt
    34321 gccagggcgc tacgaatttc agaaatcacc tggctgggtt cgtttgttgc gtcgatgata
    34381 atatgcgcaa cttcgcgata tagcgcatcg cgttcttcca gcacttcctg aacttcttcg
    34441 ctcagaggtt ttcccgttaa ggttggccgt aaatcttctt ccggtgcagc ttgcagtcgg
    34501 ttaaccagga ctgaaactgg cgcacacaaa taaaccacga tcccgttatt ttgcatgaag
    34561 tgacgattaa attccgtcag aataatgccg ccgcctgtag cgataacggt ggatggcgca
    34621 gttaccgctt ccagcgccgc cgtttctctg gcgcgaaatc ccgcccactc ttccctttcg
    34681 acgatctccg cgaccgtcat attgagctgt gattgcaacc actgatcggt atcgacaaac
    34741 cgacggttaa gcgaatcggc aagggccatt ccgaccgttg ttttaccaca gccccgaggc
    34801 ccgatcagaa aaagaggttg tgtcatcgtg ggttttcccc aataggtcgc aatgcggcga
    34861 aagccggtgt catgagaata gcgatcatac catcaaacta gtacaatttc gattgtaaag
    34921 aaaaaattcc acttaaagtg aaaatctcaa tacatccctt actataccaa taaatattca
    34981 agaatgaagt gtaaataata aattacattt agccacgact acgttgcact tccagccacc
    35041 acttctccag ctccgcggca aatgcctggc ggtcacgttg tgaaaggcta tctggtccgc
    35101 cggtctggat cccactggca cgtaaggtat ccataaaatc gcgcatcgtc aggcgctcac
    35161 gaatggtcgc tggcgtgtaa cgttcaccgc gcggattaag cgccgcagcg cctttctcga
    35221 tggcttcagc agccaaaggt atatctgcgg tgatcaccaa atcgcccgct tcacactgcc
    35281 ggacaatttc gttatcggca acgtcgaaac ctgccgcgac gcgcagcgta cgaataaatc
    35341 gcgatggcgg cacgcgtaaa ctctggtttg ctaccagtac cagcggcatc tgcatacgtt
    35401 ccgccgcgcg atacaaaatc tctttaatta cattgggaca cgcgtcggca tccacccaaa
    35461 ttgtcataaa gtcatccttt gttgggtaat cctctattgt gtcgcgcttt tgccttccgg
    35521 catagttctg tttatgcttc tgccagcgat tatcaaaaca atgaatttca cggcaggagt
    35581 gaggcaatgg aaaagaaaat cggttttatt ggctgcggca atatgggaaa agccattctc
    35641 ggcggtctga ttgccagcgg tcaggtgctt ccagggcaaa tctgggtata taccccctcc
    35701 ccggataaag tcgccgccct gcatgaccag ttcggcatca acgccgcaga atcggcgcaa
    35761 gaagtggcgc aaatcgccga catcattttt gctgccgtta aacctggcat catgattaaa
    35821 gtgcttagcg aaatcacctc cagcctgaat aaagactctc tggtcgtttc tattgctgca
    35881 ggtgtcacgc tcgaccagct tgcccgcgcg ctgggccatg atcggaaaat tatccgcgcc
    35941 atgccgaaca ctcccgcgct ggttaatgcc gggatgacct cggtaacgcc aaacgcgctg
    36001 gtaaccccag aagataccgc tgatgtgctg aatattttcc gctgctttgg cgaagcggaa
    36061 gtaattgctg agccgatgat ccacccggtg gtcggtgtga gcggttcttc gccagcctac
    36121 gtatttatgt ttatcgaagc gatggccgac gccgccgtgc tgggcgggat gccacgctcc
    36181 caggcgtata aatttgccgc tcaggcggta atgggttccg caaaaatggt gctggaaacg
    36241 ggagaacatc cgggggcact gaaagatatg gtctgctcac cgggaggcac caccattgaa
    36301 gcggtacgcg tactggaaga gaaaggcttc cgtgctgcag tgatcgaagc gatgacgaag
    36361 tgtatggaaa aatcagaaaa actcagcaaa tcctgatgac tttcgccgga cgtcaggccg
    36421 ccacttcggt gcggttacgt ccggctttct ttgctttgta gagcgccaaa tctgccgatt
    36481 tcaaccactc acgatagtga ctcatttgtg ggttcagcgg cgcaaccccc acactaatcc
    36541 gtaaagttac ctgtggcgta ttcggcaaac gtaatgtatt tagcccttca tgcacccgta
    36601 acatggcggt aatggcgctc tcagctggcg taccggacat gattactgca aactcatcgc
    36661 cgccaaaccg accaatcaca tcgctaccac gcagggtaat ttgtaactgt cgggtaagcg
    36721 ccacaatcgc ttcatcgccc acatcatggc cccaggtatc gttgatgctc ttgaaatggt
    36781 cgatatcgat aatcagtaac gttgcatcgc gattatgccg ccgacagtta tcaaattcat
    36841 tgcgtaacat agtttcccaa tgacgtcggt tatacacgcc cgtcatgccg tcgcgggtac
    36901 tcatgacctg caacctgcgt ttatgttccg ccagtttagt cgccgtctgg tagctgaccc
    36961 agccaaacag cagaggataa atgacaataa tgggaaggga gagccaccat tccagcggcg
    37021 cactattgaa cgacacggtg atgcccgtca gctcgagggt gacaaggcag gaaaccacca
    37081 tcaacaccag acccgcgaca aacagacggg ggccgcctgc ccccatcaaa ttcagacaca
    37141 taatcatcaa catcgcggtg gaaggcagca cgtttacgcc cattacgcct acccacattc
    37201 ccgctaatac tgcatcggtt tttaagttgt aaatttcccg gctaagcgga tcgacggccc
    37261 tgctcgctat ctgccaggct aaatgcggcc agacgaacgc ccagccgacc aacaccagcc
    37321 accaccagcc cggcggcggg tgtgaaacca gcgttgaagc aatcggtaag aacatgccag
    37381 ccaggccaac cgcacggggt agtctgacgc gacgggcgaa gcgcagcccg gaccgctgat
    37441 gttcgttttg aggagttaaa ggaggttcct ccccgtgcgc cgccgctttt ttgaaaaagt
    37501 tttcatcatt cattattttt gggaacattc agaaacaatt ttcccaaatt atagagacgg
    37561 ggctaaaggc agaagtatga aatttcgggt gagccatttt agtagctcac ccgttagcac
    37621 tatcaggtat caggcggctt tcttgaggca ggcgctcata aacttattac gatcatcacc
    37681 tttcagtgat tgttgcgttg cttgattatt acattcgcgc atcttttgct gctgtggtgt
    37741 caaacttttt tcaccaggcg cagacttgct gttcttcagg caatcactca tgtaggtctt
    37801 acgagcatcc cctttcaacg cctgtgccgt cgcctgctga ttacaggagg tcatacgctg
    37861 ttgttgtggg gttaaggttc tctcggcagc gccgacggtg gttaaaaaaa ccagaccgaa
    37921 aagcaaggta accagtaatg taattttcat agcaccatcc ctcttcatgt tttaaccatg
    37981 agcgtatgcg cccgtgatct gccattaagt ctggttgcta acagcaaaaa aaccacccgg
    38041 cagcgaaaat tcactgccgg gagcggtttt atttcagccc cagagcggct ttcatggtgt
    38101 agaagagatc ggtctggtcg gtcagtccaa cgacattggc ggcatgcggg ccatacgccg
    38161 caatacgcaa ctgactgccg gtatgttctt gtgaatcctc ttcggagttc ccgtaactca
    38221 tcaccatcac tgcgccatct ttggtattta gcgcctgggt gaggcccgga gctttggtat
    38281 ccggcgcaac aatctggctg gcgtgggcgt gatcagcggt gactatgacc agcgtgttac
    38341 catccttttt ggcgaattcc agcgcccgtt gtacggcttc atcgagatcg accgtctcgc
    38401 caatttgccc acaaggattc gcagcatgat cctgtttatc gattgacgca ccttcaactt
    38461 gcaggaaaaa gcctttctca tttttactca ataattcaat ggctttgtcg gtcatctgtg
    38521 ccagggtcgg tacactgtca ttacgttgcg gattaggcgt acaggtgact gcgggcttat
    38581 cgatattacc gtggtacgtt gctttcggtc ctagccagcg cactggcata ttgccgtcag
    38641 caaacaggcc aagcaggggt ttttgctgat tcgcttccgt caccgaattc agtgaggcag
    38701 catcgctcac caactgataa ccacgcgcct gtgcctgttc acgcagcgtt tttccctgcc
    38761 attcaccagc ggttgccgtt tcagcaaagg tttttgcgcc gccgccaagc gtaacgtcgg
    38821 cacgagcgtt aagcagctgt tcggtaatcg atccttttcc gcctttttcc agagcgttac
    38881 ccggacattt ttcactggtc gcgctcggac cgtagcattt gcgcgaagtc acatgtgcca
    38941 ccagcgcagc gggcgtggca tcctgcaact ctgcggtaga aacgttaccg gtcgccagac
    39001 ctgcggcttt tgccatttcc agaatcgttg ggtgatcttt ttcgtgaata tcgacgccca
    39061 gcgcgccgtt ataggttttg acaccggttg accaggcggt tgctgatgca gccgagtcgg
    39121 tgacgtagtc cggtttgccg gtttttttat tcagcgcata gtgagtgtat tgcccggtaa
    39181 gcggtaaggc atcgatacca ttaaaaaagc cgcccgcacc ttcggcataa ttacgtgcgg
    39241 cagtaatttc cgaatccccc atcccatcgc caatcagcaa aataatattt tttgcaggtt
    39301 tatcgctaag agaatcacgc agagcggcgg tctgatcagc agttaagcgg cgagcaccgc
    39361 cgggtgcagt aatatcgccc tgagcagccc ggttttccag aacaggcatt tctggtgtcc
    39421 gggcttttgt cacaggggta aacagtaacg gtaagagtgc cagtgcaata gtgctttgtt
    39481 tcactttatt ttctccatgt acaaatacat taaaaaataa aaacaaagcg actataagtc
    39541 tcggccgtga caactttatg acatctgttg aaaagattaa ctttttactg acgaggatgc
    39601 ttcaataact tctttacgta atcgcgcagc agctccgtat cgtcgtcagg aatgctggca
    39661 tcgggcttta cctcgtacag cgccccctct acctgatcaa tcaaccgttg ttggtcattt
    39721 tgcgccatat tgcgaagcat tgcagtgacg ataatctcca aagcttctac ctgcgcacac
    39781 agttctttcg actcttcttc tttttgggca agcttaaata acaactcagc aatgagattt
    39841 ttcatgtctg tatttcctta tccaaagtat ggagaagtta acattatgtt tcacaacttc
    39901 atagtgtctg gaagtattat ttttatgaca aaggaaatac tttgcgcaaa tattttttgt
    39961 ttgattacca gcgaaacgtt t
//
LOCUS       DAZFKF010000039        38333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFKF010000039 DAZFKF010000000
VERSION     DAZFKF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.039"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..938
                     /gene="ytfI"
                     /locus_tag="ACHKA1_002438"
                     /pseudo
     CDS             1..938
                     /gene="ytfI"
                     /locus_tag="ACHKA1_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="protein YtfI"
     gene            complement(1001..1555)
                     /gene="ytfJ"
                     /locus_tag="ACHKA1_002439"
     CDS             complement(1001..1555)
                     /gene="ytfJ"
                     /locus_tag="ACHKA1_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO5680078.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            1880..2086
                     /gene="ytfK"
                     /locus_tag="ACHKA1_002440"
     CDS             1880..2086
                     /gene="ytfK"
                     /locus_tag="ACHKA1_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO5680079.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(2148..3491)
                     /gene="paeA"
                     /locus_tag="ACHKA1_002441"
     CDS             complement(2148..3491)
                     /gene="paeA"
                     /locus_tag="ACHKA1_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO5680080.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3814..4452)
                     /gene="msrA"
                     /locus_tag="ACHKA1_002442"
     CDS             complement(3814..4452)
                     /gene="msrA"
                     /locus_tag="ACHKA1_002442"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO5680081.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4658..6391
                     /gene="tamA"
                     /locus_tag="ACHKA1_002443"
     CDS             4658..6391
                     /gene="tamA"
                     /locus_tag="ACHKA1_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO5680082.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6388..10167
                     /gene="tamB"
                     /locus_tag="ACHKA1_002444"
     CDS             6388..10167
                     /gene="tamB"
                     /locus_tag="ACHKA1_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO5680083.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRAALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMDVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDITKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLSAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            10170..10511
                     /gene="ytfP"
                     /locus_tag="ACHKA1_002445"
     CDS             10170..10511
                     /gene="ytfP"
                     /locus_tag="ACHKA1_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO5680084.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            10723..10974
                     /gene="chpS"
                     /locus_tag="ACHKA1_002446"
     CDS             10723..10974
                     /gene="chpS"
                     /locus_tag="ACHKA1_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO5680085.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            10968..11318
                     /gene="chpB"
                     /locus_tag="ACHKA1_002447"
     CDS             10968..11318
                     /gene="chpB"
                     /locus_tag="ACHKA1_002447"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO5680086.1"
                     /translation="MVKKSEFERGDIVLVGFDPASSHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(11398..11928)
                     /gene="ppa"
                     /locus_tag="ACHKA1_002448"
     CDS             complement(11398..11928)
                     /gene="ppa"
                     /locus_tag="ACHKA1_002448"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO5680087.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            12238..13194
                     /gene="ytfQ"
                     /locus_tag="ACHKA1_002449"
     CDS             12238..13194
                     /gene="ytfQ"
                     /locus_tag="ACHKA1_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO5680088.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            13334..14836
                     /gene="ytfR"
                     /locus_tag="ACHKA1_002450"
     CDS             13334..14836
                     /gene="ytfR"
                     /locus_tag="ACHKA1_002450"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO5680089.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQPISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIEMLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            14850..15872
                     /gene="ytfT"
                     /locus_tag="ACHKA1_002451"
     CDS             14850..15872
                     /gene="ytfT"
                     /locus_tag="ACHKA1_002451"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO5680090.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            15859..16854
                     /gene="yjfF"
                     /locus_tag="ACHKA1_002452"
     CDS             15859..16854
                     /gene="yjfF"
                     /locus_tag="ACHKA1_002452"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO5680091.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLISEESIPINHPVYDTLSSLVWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQVGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(16887..17885)
                     /gene="fbp"
                     /locus_tag="ACHKA1_002453"
     CDS             complement(16887..17885)
                     /gene="fbp"
                     /locus_tag="ACHKA1_002453"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO5680092.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            18061..19434
                     /gene="mpl"
                     /locus_tag="ACHKA1_002454"
     CDS             18061..19434
                     /gene="mpl"
                     /locus_tag="ACHKA1_002454"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO5680093.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQLDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(19590..20141)
                     /gene="yjgA"
                     /locus_tag="ACHKA1_002455"
     CDS             complement(19590..20141)
                     /gene="yjgA"
                     /locus_tag="ACHKA1_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO5680094.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            20235..21587
                     /gene="pmbA"
                     /locus_tag="ACHKA1_002456"
     CDS             20235..21587
                     /gene="pmbA"
                     /locus_tag="ACHKA1_002456"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO5680095.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            21771..22157
                     /gene="cybC"
                     /locus_tag="ACHKA1_002457"
     CDS             21771..22157
                     /gene="cybC"
                     /locus_tag="ACHKA1_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO5680096.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(22202..22666)
                     /gene="nrdG"
                     /locus_tag="ACHKA1_002458"
     CDS             complement(22202..22666)
                     /gene="nrdG"
                     /locus_tag="ACHKA1_002458"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO5680097.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(22856..24994)
                     /gene="nrdD"
                     /locus_tag="ACHKA1_002459"
     CDS             complement(22856..24994)
                     /gene="nrdD"
                     /locus_tag="ACHKA1_002459"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO5680098.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(25388..27043)
                     /gene="treC"
                     /locus_tag="ACHKA1_002460"
     CDS             complement(25388..27043)
                     /gene="treC"
                     /locus_tag="ACHKA1_002460"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO5680099.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPEQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(27093..28514)
                     /gene="treB"
                     /locus_tag="ACHKA1_002461"
     CDS             complement(27093..28514)
                     /gene="treB"
                     /locus_tag="ACHKA1_002461"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO5680100.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(28633..29580)
                     /gene="treR"
                     /locus_tag="ACHKA1_002462"
     CDS             complement(28633..29580)
                     /gene="treR"
                     /locus_tag="ACHKA1_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO5680101.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            29765..29818
                     /gene="mgtL"
                     /locus_tag="ACHKA1_002463"
     CDS             29765..29818
                     /gene="mgtL"
                     /locus_tag="ACHKA1_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO5680102.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            29959..32655
                     /gene="mgtA"
                     /locus_tag="ACHKA1_002464"
     CDS             29959..32655
                     /gene="mgtA"
                     /locus_tag="ACHKA1_002464"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO5680103.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(32861..33247)
                     /gene="ridA"
                     /locus_tag="ACHKA1_002465"
     CDS             complement(32861..33247)
                     /gene="ridA"
                     /locus_tag="ACHKA1_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO5680104.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(33320..33781)
                     /gene="pyrI"
                     /locus_tag="ACHKA1_002466"
     CDS             complement(33320..33781)
                     /gene="pyrI"
                     /locus_tag="ACHKA1_002466"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO5680105.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(33794..34729)
                     /gene="pyrB"
                     /locus_tag="ACHKA1_002467"
     CDS             complement(33794..34729)
                     /gene="pyrB"
                     /locus_tag="ACHKA1_002467"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO5680106.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(34733..34867)
                     /gene="pyrL"
                     /locus_tag="ACHKA1_002468"
     CDS             complement(34733..34867)
                     /gene="pyrL"
                     /locus_tag="ACHKA1_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO5680107.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(35148..35543)
                     /gene="yjgH"
                     /locus_tag="ACHKA1_002469"
     CDS             complement(35148..35543)
                     /gene="yjgH"
                     /locus_tag="ACHKA1_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO5680108.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGS
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDNIIDITSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(35674..36387)
                     /gene="bdcA"
                     /locus_tag="ACHKA1_002470"
     CDS             complement(35674..36387)
                     /gene="bdcA"
                     /locus_tag="ACHKA1_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5680109.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDHL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHGFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            36458..37051
                     /gene="bdcR"
                     /locus_tag="ACHKA1_002471"
     CDS             36458..37051
                     /gene="bdcR"
                     /locus_tag="ACHKA1_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO5680110.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGGAIKTILKE"
     gene            37196..37648
                     /gene="tabA"
                     /locus_tag="ACHKA1_002472"
     CDS             37196..37648
                     /gene="tabA"
                     /locus_tag="ACHKA1_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO5680111.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTVETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            37771..>38333
                     /gene="yjgL"
                     /locus_tag="ACHKA1_002473"
     CDS             37771..>38333
                     /gene="yjgL"
                     /locus_tag="ACHKA1_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HFO5680112.1"
                     /translation="MSKISDLNYSQHITSADNFKPKNEVLNTWCVGMNDFARIAGGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADSSKVIIEISLSTTTMGTRQGHTAIIFPQSDFLSFRYEGKSFERKDDS
                     SLHLITNKVLACYQREANKEIARLLNN"
BASE COUNT         9284 a         9456 c        10272 g         9321 t
ORIGIN      
        1 tgttacccag gatcagacac aataatttta ttggtgcggt gtagttattt gtaaaatcat
       61 cgcatacaaa aacacattca aacaattttt ttaataatat tcagcatgca tttaagaaaa
      121 aagattggat ttcgaattat gatagcctgt taactttgag ggagttcttt cgttgcgcaa
      181 ctcagattga taaagatagt tatcaagtgc tttcatcaaa aaatggaact gttaacgcta
      241 tggataagtt tcttatcagt ttcagcctta aagataacgg tgccgaatat acaatgacat
      301 tgcgaggcag tggatttgaa tacgaagaaa cccccataac aataaatgag tataactcat
      361 tcatggattt caaaaaccgt gaatttccgt tagaacaaaa cagacgattg tatgcctggg
      421 atattctgca gaaaaaacag tctgacatac cgaaaaggat taaaggttat attcaccagg
      481 cgattggtga cgtgtcttta ggttatgcct tattggagga tatagtaagt aaattaaaac
      541 ggggtaaatt tgaactccaa ataccaggag gtggaattaa agaatgcgat ggatggtata
      601 tttacgaaaa aataattgat gatgattttg ccatagtcat agaatctttg ggctttgcgc
      661 ttaaaatcta tggaggggat gaacgttttc gtaatggttc ctctgttgtt ttggaggatg
      721 aagattactc tcttatttat aattttctgg ttaatgcagg ttgccaacaa gtcgaattag
      781 ccgaacaagt ggatgcgata gtaagtgcga atctggccgc agatagtgat attaccaaag
      841 aaaagatttg cgagaaatat aaatcgacca ttgaggcgtt taaaaaagag caactagcgt
      901 taccagtatt agttcggcgt aagaactcag agacttaatt cccctatccc atagataacg
      961 ataggggaaa attatttttg cagaatttat gttattgcca ttatttgagc aacttttgca
     1021 gcaggtccat cacttgctgc acctcttcct gagtgagcgc cccgtctttg gcccattgca
     1081 cgcgaccatc cttatccagc accactacag cggaactttc ttcatccagc tgccaggcac
     1141 cgcgcgcgac gccattgcta tcgacaataa actgcgacca gggataaagc tttttattac
     1201 tctccagact gctgcgcaca aacatgccgg aacccggaat cgcgtcgtcg gtgttaacaa
     1261 tggtggtggt ctggtaacga tcgtgcggta acttcgctga tttaatcgct tcaatcagcg
     1321 tcgcgttttt ctcttttgca gaggtgcgac cagcaatatg ttgcagtact cgcacttttc
     1381 ccactaactg cgcgctgttc caggttttgt agctaaactg atctttatca agcaccaact
     1441 cgccccgatc ggtaatgcca atcggcggca ctcgctgacc ggtttcgaac tgatgtgcgg
     1501 aagccatcat cggcaacagc aggcaggtga gtgccagaat cttgcgtagg gtcatggtgt
     1561 ttccttctta tgatatgcag gtgatccgac cacttgggtc tgagttttaa tcatatgtgc
     1621 tatttatcga atttcccgca agtgtgatgc cagtttgcgg tcaagcgcac aaatcatatg
     1681 aaaaatgaat gcttatactg aagaccgcgc ttcggtaaaa agataattct gaataattgt
     1741 aacctttagg taaaaaaagt tatacgcggt ggaaacattg cccggatagt ctatagtcac
     1801 taagcattaa aatttgcgcc tcataatagt tgggccgatt gtggcaccgc acaggcgtaa
     1861 tactcagcag gagataacaa tgaaaatttt ccaacggtac aacccacttc aggtggcgaa
     1921 gtacgtaaag atcctgttcc gtggacggtt atacatcaag gacgttggcg cttttgaatt
     1981 tgataagggt aagattctta tcccaaaagt gaaggataag ctgcatttgt cagtgatgtc
     2041 cgaagttaac cgtcaggtta tgcgtctgca aacagagatg gcttaaccaa agtgctatgc
     2101 agtaataaga cggctcctga ttcaggagcc gttgatgttt atgggggtta cgcgacgctt
     2161 tcttctttat ctttagcgtc aggcagtttt ggcgaaaggg ctgtggcctt gctgtcgatc
     2221 cgggtcacca gcagctggtc gatgcggtag ttatcgatat ccaccacttc aaatttgtag
     2281 ccggcgaatt tcaccgaatc ggtgcgtttc gggatcttac gcagcataaa catcataaag
     2341 ccgccgatgg tttcgtagtt gcccgactgc gggaactcgt caatatccag cacgcgcatg
     2401 acgtcgtcaa tcggagtgcc gccgtcaatc agccatgagt tctcatcacg ggcgacaatc
     2461 tgctcttcca gtccctgacc gaccagatcg cccatcagcg tggtcatcac gtcgttgagg
     2521 gtgatgatcc ccactaccag cgcgtactcg ttcatgatca ccgcgaagtc ttctcctgcg
     2581 gttttaaaac tttccaacgc ctctgaaagg gttaacgtat ccggcacaat cagcgtgttg
     2641 cgaatttgta cgccgctgtt cagtgccagg ctttggttag ccagcacgcg gttcagcagg
     2701 tctttagaat cgacatagcc gatgatgtgg tcaatatctt cattacagac gaggaactta
     2761 gagtgcggat gttccgccac cttatttttc agactttgct catcttcgtg gagatcaaac
     2821 caaatcacgt tttcacgcgg tgtcattgaa gacggaacgg tacgggattc cagctcaaag
     2881 acgttttcaa tcagctcgtg ttcctgttta cgtaacacgc ccgccagcgc accggcttcc
     2941 actaccgcgt agatgtcatc agaagtgatg tcatctttac gtaccattgg cagtttgaaa
     3001 atacggaaga ttatgttcgc caggccgttg aagaaccaca ccagcggggt gcaaacgtac
     3061 aggcagaagc gcatcgggtt gatgatacgc aaagccacag cttctggcgc aatcataccg
     3121 atgcgtttcg gggttaaatc cgcaaacagg ataaacatgc cagtcactaa cgagaaagag
     3181 agaataaagc tcagttgctc agagagctct gccgacatat agcgggagaa caggctgtga
     3241 aaagctggag aaaatgccgc atcaccgacg ataccgccga gaatcgccac tgcgttcaga
     3301 ccgatttgga ccacggtaaa gaacatgccg ggattttcct gcatattcag aacgcgttgg
     3361 gcatttatat tgccttcatc agccagcagt ttaagtttga ttttgcgtga ggcggcaagc
     3421 gagatctcgg acatcgagaa gaacgcactt acagcgatca agcagagtat gactaaaata
     3481 ctgtttaaca tatcttatcc gacctttcag gccagatcct cggaagggga agttgattaa
     3541 tttgtgtgaa atacacattg aaagccgatt cgaagaaagt gaaccggcat tttcagtggc
     3601 tagtatagcg taaggtactg taaagtcgcc agagggttaa aattcagact gctgataaat
     3661 gcaatactgc ctgatgcgct atgccgatca ggcctacgca gtccttgcaa tatattgaat
     3721 ttggacgatt ttgtaggcct ggtaaggcgt ttacgccgca tccggcataa ataaagcgca
     3781 ctttatcaac aatctacatt tgtacccgta acgctatgct tccggtggca gacagacgcc
     3841 aattccgcca attccacagt aaccatacgg gtttttatgc agatattgct ggtggtcatc
     3901 ttcggcataa taaaacggtg tggcgttagc gatttccgtg gtgatgtgac gatcgtcatc
     3961 ggcggcaagc atcgccgcct gaaaacgttc cagactggcg cgagctgcgg catcctgttc
     4021 tggggtcagc ggataaatcg ctgaacgata ctgcgtgccg tggtcattgc cctgacgcat
     4081 tccctgggcg ggatcgtgat tctcccaaaa tacctgtagc aactgctcat agctgatgac
     4141 ggaaggatcg taaacaatgc gtaccgcttc ggcatgaccc gtatcaccgg agcacacttc
     4201 ccgataagtc ggatttggcg tgtagccgcc ggtatagcct gcggcggtgc tgtaaacgcc
     4261 gggtaactgc cagaacagac gctccacacc ccagaaacaa cccatcgcaa aaatggcaat
     4321 ctccattccg tcaggtacat tggtcattga gtgaccgttg accgcatgca gcgtggctac
     4381 gggcatcggg gtgttacgtc caggcagggc atcggcgggg gaaaccagat gctttttatc
     4441 aaataaactc atggtgtcgc tctcccgaaa atcggtcatt ggggttaagg ttgtaacaag
     4501 aggcgtattt gcacacaata accaccgtga ataggtctaa agtaaaacat aagaaatatt
     4561 tggggtttag tctgcttttt aatccatatt actggatttt tgttaagccg tttaacggcg
     4621 ttccaggggc aggaaaaaag gatattcagg agaaaatgtg cgctatatcc gacagttatg
     4681 ctgtgtaagc ttactctgct taagcggatc tgccgtcgcc gcgaacgtcc gtctacaggt
     4741 cgaggggtta tcgggacagc tggaaaagaa cgttcgcgcg cagctttcta cgattgaaag
     4801 tgatgaagtg acgccagacc gtcgctttcg cgcacgcgtc gatgatgcta tccgcgaagg
     4861 tctgaaagcg ctgggttatt accagccgac cattgaattt gatctccgtc caccgccaaa
     4921 gaaagggcgg caggtattga tcgccaaagt cacgccaggc gtgccggtgt taattggcgg
     4981 caccgatgtg gtattgcgcg gcggcgcgcg gaccgataaa gactatttga aattgctcga
     5041 tactcgcccg gctattggta cggtactgaa ccagggcgat tatgaaaatt tcaaaaagtc
     5101 cttaaccagc attgcgttgc gtaaaggtta tttcgatagc gaatttacca aagcgcagct
     5161 gggcattgcg ctcggcctgc ataaagcctt ctgggatatt gattataaca gtggcgaacg
     5221 ttaccgcttt gggcatgtga cctttgaagg atcacaaatc cgcgatgaat acctgcaaaa
     5281 tctggtgccg tttaaagagg gcgatgagta cgaatcgaaa gatctggcag aactgaaccg
     5341 ccgactttct gctaccggct ggtttaactc ggtggtggtg gctccacaat ttgataaagc
     5401 gcgcgaaacg aaagtattac cattgacggg cgtggtttcg ccgcgaacag aaaacaccat
     5461 cgaaaccggg gtcggttact ctacggacgt gggaccgcgc gtgaaagcga cgtggaaaaa
     5521 gccgtggatg aactcttatg gtcacagtct gaccaccagt accagtattt ccgcgccgga
     5581 acagaccctc gacttcagct ataaaatgcc gctgctgaag aatccactgg aacaatatta
     5641 tttggtgcag ggcggtttta agcgcactga cctgaacgat accgagtctg actccactac
     5701 gctggtggct tctcgctact gggatctctc cagcggctgg cagcgtgcca ttaacctgcg
     5761 ctggagtctc gaccacttta ctcagggtga aattaccaac accacgatgc tgttttatcc
     5821 tggggtgatg attagccgca cgcgttctcg tggtggcctg atgccaacct ggggcgactc
     5881 gcaacgctac tctatcgact actccaacac tgcctggggc tcagatgtcg atttctccgt
     5941 tttccaggca caaaacgtct ggatccgcac actgtacgat cgccatcgtt ttgtgacacg
     6001 cggcacgctg ggctggattg aaaccggtga tttcgacaaa gtaccgccgg atctgcgttt
     6061 tttcgccggg ggcgaccgca gtattcgtgg ctacaaatac aaatctatcg ctccgaaata
     6121 cgccaacggt gacctgaaag gggcctcgaa gttgataacc ggatcgctgg agtaccagta
     6181 caacgtgacc ggaaaatggt ggggcgcggt gtttgtcgat agtggcgaag cggtaagcga
     6241 tattcgccgc agcgacttta aaaccggtac cggggtcggc gtacgctggg aatcgccggt
     6301 cgggccaatc aaactcgatt ttgccgtacc ggtcgcggat aaagacgaac acgggttaca
     6361 gttttacatc ggtctggggc cagaattatg agtttatgga aaaaaatcag cctcggcgtg
     6421 gttatcgtta tcttactgtt gctgggatcg gtggcgtttc tggtgggcac caccagcggc
     6481 ctgcatctgg tatttaaagc ggcggatcgc tgggtgccag gactggatat tggcaaggtc
     6541 accggcggct ggcgcgatct caccttgtct gacgttcgtt atgagcagcc aggcgtggcg
     6601 gtaaaagcag gcaatctgca tctggcggtc gggcttgagt gcctgtggaa cagcagtgtt
     6661 tgtattaatg atctggcgct gaaagacatc caggtcaata tcgacagcaa aaaaatgccc
     6721 ccttctgaac aggttgaaga agaggaagat agcggtccgc tggatctctc cacgccgtat
     6781 cccatcaccc tgacacgggc ggcactggac aacgtcaaca tcaagattga tgacaccacg
     6841 gtatcggtga tggacttcac ctccggcctg aactggcagg agaaaaccct gaccctgaaa
     6901 ccgacgtcgc tgaaaggcct gctgattgct ctgccgaaag tggcggaagt ggcgcaggaa
     6961 gaagtggtcg aaccgaaaat tgaaaatccg cagccggagg aaaagccgct cggcgaaacg
     7021 ctgaaagatc tcttttctcg cccggtattg ccggaaatga ccgacgtgca tttgccgctt
     7081 aacctgaaca ttgaagagtt taagggcgag cagttgcgcg tgacgggcga cacggacatc
     7141 accgtgcgca ccatgctgct gaaagtgagc agcattgacg gcaatactaa actggacgcc
     7201 ctggatatcg attccaacca ggggatcgtc aacgccagcg gcacggcgca gctgtcagac
     7261 aactggccgg tggacatcac tctcaacagt acactgaacg tggagccgtt gaaaggtgaa
     7321 aaagtgaagc tgaaagtggg cggcgcgctg cgcgaacagc tggagattgg cgttaatctt
     7381 tccggtccgg tggatatgga tttacgcgcc cagacgcgac tggcggaagc cggattgccg
     7441 ctcaacgtgg aagtgaacag caaacagatt tactggccgt tcactggtga gaagcagtat
     7501 caggcggatg atctgaaact gaaacttacc ggcaaaatga ccgattacac gctctctatg
     7561 cgtacggcag tgaagggaca ggagatcccg cccgcgacta ttaccctcga cgccaaaggt
     7621 aatgaacagc aggtcaatct cgacaaactc accgtcgcgg cgctggaagg gaaaactgaa
     7681 ctcaaggcgt tgctcgactg gcagcaggcc attagttggc gcggtgagct aacgcttaat
     7741 ggcattaaca ccgccaaaga gttcccagag tggccgtcga aactcaatgg gttgattaaa
     7801 acccgcggta gcctgtacgg cggcacctgg cagatggacg tgccggagtt aaagctgacc
     7861 ggtaacgtta aacagaacaa agtgaacgtt gacggcacgc tgaaaggcaa cagttatatg
     7921 cagtggatga tcccagggct tcatctggaa ctcgggccaa acagtgccga agtgaaaggc
     7981 gagctggggg taaaagatct caatcttgat gccaccatca acgcgccggg gctggataac
     8041 gcgctgccgg ggcttggcgg tacagcgaaa gggctggtga aagtacgcgg cacggtggaa
     8101 gcgccacaac tactggcaga tatcaccgcg cgcggcctgc gctggcagga actttccgtg
     8161 gcgcaggttc gcgtggaagg cgacattaaa tccaccgatc agatcgccgg gaaactcgac
     8221 gtacgcgttg agcaaatttc gcagccggat gtaaatatca acctcgtcac cctgaatgcc
     8281 aaaggcagcg aaaagcagca cgagctacag ttgcggattc agggcgagcc tgtctccggg
     8341 cagcttaatc tggcaggaag ttttgatcgc aaagaagaac gctggaaggg aacacttagc
     8401 aatacccgct tccagacgcc ggtcggcccg tggtcgctga cccgcgatat tgcgctggat
     8461 taccgcaata aggagcaaaa aatcagcatc gggccacact gctggcttaa cccgaatgcg
     8521 gaactgtgcg tgccgcaaac tatcgatgcg ggggccgaag ggcgtgcggt ggtgaatctc
     8581 aaccgcttcg acctcgccat gctgaaaccg tttatgccag aaaccactca ggccagcggt
     8641 atcttcacgg gtaaagcgga cgttgcctgg gacatcacga aagaggggct gccgcagggc
     8701 agtatcactc tttcggggcg taacgttcag gtaacgcaaa ccgtcaacga tgcggcgctg
     8761 cccgtggcgt ttcagacgtt gaatctgtcg gcggagttgc gtaacaaccg tgccgaattg
     8821 ggctggacca tccgcctgac caataacggc cagtttgatg gacaggtgca ggtgaccgat
     8881 ccgcaaggcc gccgtaatct tggtggcaac gtcaatatcc gtaacttcaa ccttgcgatg
     8941 ataaacccca tctttacccg tggggaaaaa gcagcgggga tggtgagtgc caacttgcgt
     9001 ctgggtggtg atgtgcaaag cccgcagttg tttggtcagc ttcaggttac gggtgtggat
     9061 atcgatggca actttatgcc gtttgatatg caaccgagcc agcttgcggt caactttaac
     9121 ggtatgcgct cgacgcttgc cggtacagta cggacccagc agggtgaaat ctacctgaac
     9181 ggtgatgccg actggagcca aattgaaaac tggcgggcgc gggtaacggc gaaaggcagt
     9241 aaagtgcgga tcaccgtgcc gccgatggta cgaatggatg tatcgccaga tgttgtattc
     9301 gaggctacac caaacctgtt taccctcgat ggtcgcgtgg atgtcccgtg ggcgcgcatc
     9361 gtggtgcacg atctgccgga aagcgcagta ggcgtctcca gcgatgtggt gatgcttaac
     9421 gataacctgc aaccggaaga gccgaaaacg gcgtcgattc cgattaacag taacctgatt
     9481 gtccacgttg gcaacaatgt gcgcattgac gcctttggcc tgaaagcgcg gctgacgggc
     9541 gatcttaacg tcgttcagga caaacaaggg ctgggcctga acgggcagat caacatccct
     9601 gaagggcgct tccatgccta tggtcaggat ctgattgtgc gtaaaggtga gttactgttc
     9661 tctggcccgc cggatcaacc gtatcttaat attgaagcta ttcgtaaccc ggatgctaca
     9721 gaagacgacg taatcgccgg agttcgcgtc actggtctgg cggacgaacc gaaagcggag
     9781 atcttctctg acccggcgat gtcgcaacaa gctgccttgt cttatttgct acgtggacaa
     9841 gggctggaga gcgatcagag cgacagtgcg gcaatgacct cgatgctgat tggtctgggg
     9901 gttgcgcaaa gtggccagat tgtgggtaaa atcggcgaga cgtttggcgt aagcaattta
     9961 gcgctcgaca cccagggagt aggcgactcc tcccaggtag tggtcagcgg ctatgtattg
    10021 ccaggtctgc aagtgaaata cggcgtgggt atatttgact ctatagcaac actcacgtta
    10081 cgttatcgcc tgatgcctaa gctatatctg gaagccgtgt ctggtgtaga ccaggcactg
    10141 gatttgctct atcagttcga gttttagcaa tgcgaatatt tgtctacggc agtttacgcc
    10201 acaaacaagg caacagtcac tggatgacca atgcccagtt actgggcgat ttcagtatcg
    10261 ataactacca gttgtatagc ctgggccact atccaggcgc agttccgggg aacggaacgg
    10321 tacacggtga agtttatcgt attgacaacg ccacgctggc cgaacttgat gccttgcgca
    10381 ccaggggcgg tgaatacgcg cgccagttga ttcagacgcc gtacgggagt gcatggatgt
    10441 acgtttatca acgacccgtc gatggattaa agctaattga aagcggcgac tggttagaca
    10501 gggataagta accctatgca tacgccacct tcgggtggcg ttgttttttg cgagacgact
    10561 cgcattctgt tttgtaattc cctcaccttt tgcttttctc tccgagccgc tttccatatc
    10621 tattaacgca taaaaaactc tgctggcatt cacaaatgcg caggggtaaa acgtttcctg
    10681 tagcaccgtg agttatactt tgtataactt aaggaggtgc agatgcgtat taccataaaa
    10741 agatggggga acagtgcagg tatggtcatt cccaatatcg taatgaaaga acttaactta
    10801 cagccggggc agagcgtgga agcgcaggtg agcaacaacc aactgattct gacacccatc
    10861 tccaggcgct actcgcttga tgaactgctg gcacagtgtg acatgaacgc cgcggaactt
    10921 agcgagcagg atgtctgggg taaatccacc cctgcgggtg acgaaatatg gtaaagaaaa
    10981 gtgaatttga acggggagac attgtgctgg ttggctttga tccagcaagc agccatgaac
    11041 agcaaggtgc tggtcgacct gcgcttgtgc tctccgttca agcctttaat caactgggaa
    11101 tgacgctggt ggcccccatt acgcagggtg gaaattttgc ccgttatgcc ggatttagcg
    11161 ttcctttaca ttgcgaagaa ggcgatgtgc acggcgtggt gctggttaat caggtgcgga
    11221 tgatggatct acgcgcccgg ctggcaaagc gtattggtct ggctgcggat gaggtggtgg
    11281 aagaggcgtt attacgcttg caggcggtgg tggaataagg tgtgtttatt tatcgcgggc
    11341 ataaaaaaac ccttactaac cgaagcccgg cgttcagggt tattgcgcta gaagaactta
    11401 tttattcttg gcgcgctcga aggaggcaac gatttcagct ttagcggctt ctgcgttttc
    11461 ccaaccttca actttcaccc acttgccttt ttcgaggtct ttgtagtgct cgaagaagtg
    11521 agcgatctgc gctttcagca gctctggcag atcgttaacg tctttaatgt gatcgtattc
    11581 tttgctcagc ttgctgtgcg gaaccgcaac cagtttcgca tcttcaccgg cttcgtcggt
    11641 cattttcaga acgccaaccg gacggcaacg gatcacagaa cccggctgca gcgggtacgg
    11701 agtcgggacc agtacgtcaa ccgggtcacc gtccagagac agggtgtggt tgatgtaacc
    11761 gtagttgcat ggatagaaca tcgcggtgga catgaagcgg tcaacgaaca gtgcgccgct
    11821 ctctttgtcg atttcgtatt tgatcggatc tgcgttagcc gggatctcaa taacaacgta
    11881 gatgtcttcc ggcagatctt tacccgcagg gacgttgagt aagctcatgt ctgtttcctt
    11941 taaaaatatg tggcaaacaa gtgccgagta ttatagccaa ctcgcgccga atgtcttcgc
    12001 ttgttttcgt cttcattttc ctttttcgcc agttttaaga cggtatacag agcaggaaaa
    12061 tccataaccg tagccgcatt tttcatagtg agatgaaagc gattacaaac ttgtgattaa
    12121 cgttttattt acttttttga agtgtgatgt aacgcaatcc gttacataac gaattgtcta
    12181 tagttttttc gcgaacatct tttcaccaat aataattacc ccgacgagga caaccctatg
    12241 tggaaacgct tacttgtagt ctctgcagtc tcggcagcca tgtcgtctat ggcgttggcc
    12301 gctccattaa ccgtaggatt ttcgcaggtc ggatcggaat ccggctggcg cgccgcagaa
    12361 accaatgtgg cgaaaagtga ggccgaaaaa cgcggaatta cgctgaaaat tgccgatggt
    12421 cagcaaaagc aggaaaacca gattaaagcg gtacgttcct tcgttgcaca aggggtggat
    12481 gcgatcttta tcgctccggt ggttgcgacg ggttgggaac cggtattaaa agaggcgaaa
    12541 gatgccgaaa tcccggtctt cttgctcgac cgttccatcg atgtgaaaga caaatctctc
    12601 tatatgacca ccgttactgc cgacaacatc ctcgaaggca agttgattgg tgactggctg
    12661 gtaaaagaag tgaatggcaa accatgcaac gtggtggagc tgcagggcac cgttggggcc
    12721 agcgtcgcca ttgaccgtaa gaaaggcttt gccgaagcca ttaagaatgc gccaaatatc
    12781 aaaatcatcc gctcacagtc aggtgacttc acccgcagta aaggcaaaga agtcatggag
    12841 agctttatca aagcggaaaa caacggcaaa aacatctgca tggtttacgc ccataacgac
    12901 gacatggtga ttggtgcaat tcaggcaatt aaagaagcgg gcctgaaacc gggcaaagat
    12961 atcctcacgg gttccattga cggtgtaccg gacatctaca aagcgatgat ggatggcgaa
    13021 gcgaacgcca gtgttgaact gacgccgaac atggcgggac ccgcttttga cgcgctggag
    13081 aaatacaaaa aagacggcac catgcctgaa aagctgacgt taaccaaatc caccctttac
    13141 ctgcctgata ccgcaaaaga agagttagag aagaagaaaa atatggggta ttgagggttg
    13201 ctatgcctga tgccgattcg taggccggat aaggcgctcg cgccgcatcc ggcgatggtg
    13261 cactgaagcc tgatgcgacg cttaccgcgt cttatcatgc ctactgggag cacgctttac
    13321 accgggggaa accatgacga ccgaccaaca ccaggagatc ctccgcaccg aaggattaag
    13381 taaatttttc cccggcgtca aagcgttaga caacgttgat ttcagcctgc gccgtggcga
    13441 aatcatggcg ctgctcggtg aaaacggggc gggaaaatca acgctaatca aagcattaac
    13501 tggtgtatac catgccgatc gcggcaccat ctggctggaa ggccagccta tctcaccgaa
    13561 aaataccgcc cacgcgcaac aactcggcat tggcaccgtc tatcaggaag taaacctgct
    13621 acccaatatg tcggtcgctg ataatctatt tataggccgc gaacccaaac gcttcggcct
    13681 tctacgccgc aaagagatgg aaaagcgcgc caccgaactg atggcatctt acggtttctc
    13741 cctcgacgtg cgcgaaccgc tcaaccgctt ttcagtcgcg atgcagcaaa tcgtcgctat
    13801 ttgccgggct atcgatctct ctgccaaagt gctgatcctc gatgaaccaa ccgccagtct
    13861 cgacacccag gaagtggagt tactgtttga cctgatgcgt cagttgcgcg atcgcggcgt
    13921 cagcctgatt tttgtcactc actttctcga tcaggtctat caggtcagcg atcggatcac
    13981 cgtcttacgc aacggcagtt tcgtaggctg tcgggaaacg tgcgagctac cgcagatcga
    14041 actggtaaaa atgatgctgg ggcgcgagct ggatacccac gcgctacagc gtgccgggcg
    14101 aacattgttg agcgacaaac ccgttgccgc gttcaaaaat tacggcaaaa aaggaacgat
    14161 cgcaccgttt gatctcgaag tacgccccgg cgagatcgtc ggtctggctg gcttgctggg
    14221 atcaggacgt accgaaaccg ccgaagtgat cttcggtatc aaacctgctg acagcggcac
    14281 tgcgttgatc aaaggcaaac cgcaaaccct gcgatcgcca catcaggcgt cggtactggg
    14341 tatcggcttc tgcccggaag acaggaaaac cgatggcatc atcgccgctg cctcggtgcg
    14401 ggaaaatatc atcctcgcgc tacaggccca gcgcggctgg ctgcgaccga tctcccgcaa
    14461 agaacagcaa gagattgccg aacgctttat ccgccagctt ggcattcgca ccccttccac
    14521 tgaacaaccg attgaatttc tctccggcgg caatcagcaa aaagtgctgc tctctcgctg
    14581 gctgctgact cgtccgcaat ttctgatcct cgacgagcca acgcgtggaa ttgacgttgg
    14641 tgcgcacgcc gagatcatcc gcctgattga aatgctatgc gccgatggtc tggcgctgct
    14701 ggtgatctcc tccgaactgg aagaactggt gggctatgcc gaccgggtga tcatcatgcg
    14761 cgatcgcaaa caggtggcgg agatcccgct ggcagagctt tccgttccgg cgatcatgaa
    14821 cgccattgcg gcgtaaggag aacagtgtga tgcctcaatc tcttccggac accactacgc
    14881 caaaaaggcg ctttcgctgg ccgacgggga tgccgcagct ggtagcacta ttgctggtgc
    14941 tgctggtcga tagcctggta gccccgcatt tctggcaggt ggtgcttcag gatgggcgtt
    15001 tgttcggtag ccccatagac attcttaacc gtgcagctcc cgttgcgtta ctggcgattg
    15061 gcatgacgct ggtgatcgcc accggtggga ttgatctctc cgtaggggcg gtgatggcta
    15121 tcgccggagc cacaacggct gcgatgacgg tcgcgggatt cagcctgccg attgttttgt
    15181 taagcgccct gggcaccggc atcctggcgg gattgtggaa cggcatactg gtagcgatcc
    15241 tcaaaattca gccgtttgtc gccactctga tcctgatggt cgccgggcgc ggcgtggcgc
    15301 aactgatcac cgccgggcag atcgtcacgt ttaactcgcc ggatctctca tggttcggca
    15361 gtggatcgct gttgttcctg ccaacgccgg tcattatcgc ggtgctgacg cttatcctgt
    15421 tctggctgtt gacccgcaaa acggcgctgg ggatgtttat cgaagccgtt ggtatcaaca
    15481 ttcgggcggc aaaaaatgcc ggggtaaaca cgcggatcat cgtcatgctt acctacgtgt
    15541 tgagcgggct gtgtgcggcg attgcgggca ttatcgtggc ggcggatatt cgcggtgccg
    15601 atgccaataa cgccgggtta tggctggagc tggacgccat tctcgcggtg gtgattggcg
    15661 gcggatcgct gatgggcggg cgctttaacc tgctactttc ggtggtgggg gcgctgatta
    15721 ttcaggggat gaacaccgga attttgcttt cgggctttcc gccagagatg aaccaggttg
    15781 taaaagcggt ggtggttctt tgcgtgctga ttgtccagtc gcaacgcttt atcagtctga
    15841 ttaaaggagt acgtagccgt gataaaacgt aatttgccac tgatgatcac catcggcgtc
    15901 tttgtgttgg gttatcttta ctgcctgacc cagtttcccg gttttgcttc cacaagagtg
    15961 atctgcaata tcctgaccga taacgccttt cttgggatca ttgccgttgg catgaccttt
    16021 gtgatcctct ccggtgggat cgatctctcc gtcggttcgg tgatcgcctt taccggcgtg
    16081 tttctggcaa aagtgattgg agatttcggt ctctcaccgc tgctggcgtt tccgctggtg
    16141 ctggtgatgg gctgtgcctt cggcgcattt atggggcttt tgatcgacgc cctgaagatc
    16201 ccggcattta tcattaccct tgcagggatg ttctttttgc gcggtgtcag ctatctcatc
    16261 tcagaagagt cgatcccgat aaaccatcct gtttatgaca cgctctcaag ccttgtatgg
    16321 aaaatccctg gcggcggtcg cttaagtgcg atggggctgc tgatgctggc ggtggtggtt
    16381 atcggcatat tcctcgcgca tcgtacccgt tttggtaatc aggtatacgc cattggcggc
    16441 aatgcgacgt cggcaaacct gatggggatt tctacccgta gcactactat tcgcatttat
    16501 atgctctcca ccggactggc aacgctggcg gggattgtct tctcgattta tacccaggtc
    16561 ggatatgcgc tggcgggcgt aggtgtggaa ctggacgcta tcgcctcagt ggtgattggc
    16621 ggtacgcttt tgagcggtgg cgttggaacg gtattaggga cgctttttgg cgtggcgatt
    16681 cagggactga ttcagactta cataaacttt gatggcacgc tgagctcatg gtggacgaaa
    16741 atcgccatcg gtattttgtt gtttattttt atagcattac agcgtgggtt aacggtgctg
    16801 tgggagaatc gtcagagttc gccagtgaca agagtcaaca ttgcgcagca ataaaaacgc
    16861 ctctccgtgt ggagaggcgc aggagattac gcgtccggga actcacggat aaagcgttcg
    16921 acatcttcaa ccatatggtc gttgccgaca aagaacgaac ggcgctggtg cagggtttcc
    16981 gggataatat ccagaatacg ctctttgcca tcgctcgctt taccgcccgc ttgttccgcc
    17041 aggaatgcca tcgggttgca ctcatacagc aaacgcagtt tgccgtccgg gtggctggcg
    17101 gtgcttgggt agagataaat accgcctttc agtaggttac ggtggaaatc cgcaaccagt
    17161 gagccgatat aacgtgaggt atacgggcgg ttggtggatt tatcttcttc ctggcagaat
    17221 ttaatgtact tcttcacccc gttcggaaac ttaatgtagt ttccttcgtt gatggagtag
    17281 gttttgcctt tctccgggaa gcgcatccgt tcctggcaca ggcagaaaac gccgagcgaa
    17341 ggatcgtaag taaaggcgtg aacaccgcat ccggtggtgt aaaccagcat ggtagaggag
    17401 ccgtatacca cgtaacctgc cgcaacctgt ttgttaccag gctggaggaa atcttcttcc
    17461 gttaccggcg tgccaacagg cgtaacgcgg cggtagatgg agaaaatggt accgacagag
    17521 acgttaacat cgatgttgga cgagccatcc agggggtcca tcagcaccac gtattttgcg
    17581 tgttcacagc cttcaaagac gacaatctca tcttcttctt cagaggcaat gcccgcaacg
    17641 atatcgcgtg ctttcagtgc ggctttcagt ttttcattag cgaacaagtc gagtttctgt
    17701 tgaacctcgc cctgcacgtt ctcagcaccg ctggcaccca ggatatcaac cagtcctgct
    17761 ttgttgatat cacgatggat aatcttggcg cccagtttta ttgccgacag caaagcagtg
    17821 agctcaccgg tagcatgaga aaactcgtgc tgcttttcga caataaattc acctaacgtt
    17881 ttcataaaac tttccctgca atgtttatgg agtaaagcga ccgcaacaat cttaacaaat
    17941 aatctcaatg ttgcgctcag gtgaatcgcg ccagcaaatt gcggattatc ctgaaatgcg
    18001 tttctcactt gcccgacata tgcgtaaaat gagcggcaga ttaaaaaagg atagtgacgt
    18061 atgcgcattc atattttagg aatttgtggc acgtttatgg gcggtctggc gatgctggcg
    18121 cgccagttag gccatgaagt aacgggttcg gacgccaatg tgtatccgcc gatgagcacc
    18181 ttacttgaga agcaaggcat tgaactgatt cagggttacg atgccagcca gctcgatccg
    18241 cagctggatc tggtgattat tggcaacgcc atgacccgtg gaaatccgtg tgtggaagcg
    18301 gtactggaaa aaaacatccc ttatatgtca ggtccacagt ggctgcacga ttttgtgctg
    18361 cgcgaccgct gggtgctggc cgttgccggt acacacggca aaaccaccac cgcgggaatg
    18421 gcgacctgga ttctggaaca gtgtggttac aaaccgggct ttgtaatcgg cggtgtgccg
    18481 gggaactttg aggtttcggc gcgtctgggc gaaagcgact tctttgttat cgaagcggat
    18541 gagtacgact gcgccttctt cgacaaacgc tctaaatttg tccattactg cccgcgtacg
    18601 ctgatcctca acaaccttga gttcgatcac gccgatatct ttgacgacct gaaagcgatc
    18661 cagaaacagt tccaccatct ggtgcgtatc gttccggggc agggccgtat tatctggcca
    18721 gaaaacgaca tcaacctgaa acagaccatg gcgatgggct gctggagcga gcaggagctg
    18781 gtgggtgagc agggtcactg gcaggcgaaa aagctgacca ccgatgcttc cgaatgggaa
    18841 gttttgctgg atggcgaaaa agtgggcgaa gtgaaatggt cgctggtagg cgaacataat
    18901 atgcacaatg gcctgatggc gattgcagcg gctcgccatg ttggtgtagc gccggcagat
    18961 gccgctaacg cgctgggttc gtttattaat gctcgtcgcc gtctggagtt gcgtggtgaa
    19021 gcgaatggcg tcacggtata tgacgatttt gcccatcacc cgacggcgat tctggcaacg
    19081 ctggcggcgc tgcgtggcaa agttggtggt acggcgcgca ttattgctgt gctggaaccg
    19141 cgctcgaata ccatgaaaat ggggatctgc aaagacgatt tggcaccttc tttaggtcgt
    19201 gccgatgaag tcttcctgct gcaaccggcg catattccgt ggcaggtggc agaagtggca
    19261 gaagcctgcg ttcagcctgc acactggagt ggcgatgtgg atacgctggc agatatggtg
    19321 gtgaaaaccg ctcagcctgg cgaccatatt ctggtgatga gcaacggcgg ttttggtggg
    19381 atccatcaga aactgctgga tggtctggcg aagaaagcgg aagccgcgca gtaattcggc
    19441 cttagcctga tatagtgtgc gtaggccgga taaggcgttc acgccgcatc cggcatttga
    19501 gcatagtgcc tgatgcgacg cttgatgcgt cttatcaggc ctacaggtgc accgcatccg
    19561 gcaaaccata ctcacatcaa caacgaaaat tacccttcat tctccgccag ttcgcgcaga
    19621 tactggaaaa tctggcgtgc ggatttcggc ggcttattcc cttctttctc tttcttcgcg
    19681 ttgcggatca gagtacgcag ttgctgacga tcggcatcgg gccacagatt taatacctca
    19741 gcgatggcgt catcaccttg atcgatcaga cgatcgcgca ggttttccag tttatggaac
    19801 agcaccacct gctggttgtg acggtttttc agcttatcca gcgcctgacg aataggctct
    19861 acgtcgcgct ggcgcagcat cttaccgatg agctgcaact ggcggcggcg accttccatc
    19921 ttaatacgct gggcgagctc aatagccgcg cgcagatccg catctaacgg gatcttatcc
    19981 agcgcgtttt tccccagatc aacaatttcc gcgccaaggc gttttagctc ctcggcatca
    20041 cgtttaattt cacttttact gacccagata atttcatcgt cttcgtcttc gatgtcatca
    20101 ccgggaacgt cgtcgagcca gtcttcgggc tgcttagtca tctcaggctc cttaaaaaaa
    20161 gaggctaatg ttaccagtta agatgcgcac tgaaaaacgg ttctctgtta gacttcagag
    20221 aaactctcta cattatggca cttgcaatga aagtaatctc tcaagttgaa gcgcagcgca
    20281 agattctgga agaagcagtt tccactgcgc tggagttggc ctcaggcaaa tcggacggtg
    20341 cggaagttgc cgtcagcaag accaccggca ttagcgtaag cacgcgttat ggtgaagtgg
    20401 agaatgttga attcaatagc gatggcgcgc tggggatcac tgtttatcac cagaaccgca
    20461 aaggtagcgc atcatccacc gatttaagcc cgcaggccat tgcccgtact gtacaggcgg
    20521 cgctggatat tgcccgttat acctcgccag atccctgtgc cggcgtggca gacaaagagc
    20581 tgctggcctt tgacgcacca gatctcgact tgttccaccc tgcggaagtt tccccggatg
    20641 aagccattga actggcggcc cgcgcagaac aggcggcatt gcaggcggac aaacgcatca
    20701 ccaataccga aggtggcagc tttaacagcc actacggtgt caaagttttt ggcaacagcc
    20761 acggcatgtt gcagggttac tgctcaacgc gtcattcgct ctccagctgt gtaattgccg
    20821 aagaaaatgg cgatatggag cgtgattacg cctacaccat tggtcgtgcg atgagcgatc
    20881 tgcaaacgcc agagtgggtt ggggccgact gtgctcgccg tactttatcg cgcctgtcac
    20941 cgcgtaaact ctccaccatg aaagcgccgg tcatttttgc caatgaagtg gcaaccgggc
    21001 tttttggcca tctggtgggg gcgatagcgg gtggatcggt ttaccgtaaa tctaccttcc
    21061 tgctggattc gctgggtaaa caaattctgc cggactggct gaccattgaa gagcatccgc
    21121 atctgctgaa agggctggcg tcgacgccat tcgacagcga aggtgtgcgc accgagcgtc
    21181 gcgatattat taaagatggc atcctgactc agtggctgct gaccagctat tcggcgcgaa
    21241 aactggggct gaaaagcacc ggacatgcgg gcggtattca caactggcgg attgccggac
    21301 aaggtctaag cttcgagcag atgctcaaag agatgggcac cgggctggtg gtgacggaat
    21361 tgatgggcca gggcgtgagt gcaattaccg gtgattattc ccgtggtgcg gcgggcttct
    21421 gggtagaaaa cggcgaaatt cagtatccgg ttagcgaaat caccatcgca ggtaatttaa
    21481 aagatatgtg gcgcaatatt gtcaccgtcg gtaacgatat tgaaacacgc agtaatatac
    21541 agtgtggttc tgtgctgttg ccggagatga aaatcgccgg acagtaaaaa aactggcgcg
    21601 acctgccgcg ctataaacta aaaaattaca caaaatcatt cgcactgcat cgagacggaa
    21661 agtgaatgag ggccggggag cgtactcgca gtacgtgatc cggtcgaatg agcgtagtcg
    21721 tcgatgaggc agtgtgaagg atgaagtgta aataaaaaag gaagtgagca atgcgaaaaa
    21781 gcctgttagc tattcttgca gtctcctcgt tggtattcag ttcggcgtcg tttgccgctg
    21841 atcttgaaga caatatggaa accctcaacg acaatttaaa agtggtcgaa aaagccgata
    21901 acgcggcgca agtcaaagac gcgttaacga agatgcgcgc cgcagcgctg gatgcgcaaa
    21961 aagcaacgcc gccgaagctc gaagataaat caccggacag cccggaaatg aaagatttcc
    22021 gccacggttt cgacattctg gtcggtcaga ttgacgacgc gctgaagctg gcaaatgaag
    22081 gtaaagtaaa agaagcgcag gctgccgcag agcaactgaa aacgacccgc aacgcctatc
    22141 accagaagta tcgttaattc cttatttccc tgttgcctgc actcaggtaa cagggaactt
    22201 atcatcgcaa atgatgcacc acctgattgc tgctgccgcg ccagatcagg gaagggtctt
    22261 ttaaatcctg cacaaatttg ccgtcgacca gcacgttaat caaatcaaca acctgcattt
    22321 gcgcagcgtt gagttcgtcg agtttatagc ctgtccacac ccagatgtct ttacccggac
    22381 actcggcgcg gatgcgttgt accagtttca gaatatccgg cacgttttgc ggatgcagcg
    22441 gatcgccgcc ggagagggaa ataccctggc gtttgatacg agtgtcattc agatcgttaa
    22501 tgatctggtc ttccattgct ttagtaaatg gctgaccgga atttacccgc catgtgcttt
    22561 tgttatagca accggggcat tcatgaacac acccggagac aaacagggtg cagcgagtgc
    22621 cgggaccgtt gacgatgtcg acaggatagt actgatgata attcattatt tcgcctggct
    22681 tatcagtccg cgtgtaggct gtatcaggca atcaacgcct gatgcgacgc tgtcgcgtct
    22741 tatcaggcct acagccgttg ccaaatgtag gccggataag gcgtttacgc cgcatccggc
    22801 aatcaacgcg ggatgcgacg ctttcacatc acatccggca tttgcttagt gcagatcaac
    22861 ctatctgccc atttcccaaa tgtttaacgc ggcgcttaac ttcttcctgc ttaccagcgt
    22921 taaacggacg tgcatccggg ctacctaaat atccgcacac gcggcgagtt accgacacac
    22981 gggaggcgtc atggttacca catttcgggc aagtgaagcc tttgctggtg cactcgaact
    23041 caccggtaaa gccacactcg tagcactcat caatcggtgt attggtgccg taatacggta
    23101 catgctgata gctgtaatcc cagacatctt ccagcgcctt caggttgtgc tgaatgtttg
    23161 gatactcgcc gtagcaaatg aaaccaccgt tcgccagcgg cgggtaaggc gcttcaaagt
    23221 cgatcttgtc gtacgggttc accttcttct ccacatcgag gtggaaactg ttggtgtagt
    23281 aacctttatc ggtcacgccc ggcaccacgc caaactcagc agtatcgaga cggcagaagc
    23341 ggtcgcacag gttttcactc ggcgtgctgt agagactgaa accataaccc gtttcttctt
    23401 tccactgatc cactgcctga cgcagacgtt caacaatggc gatacctttc gcgcgaagct
    23461 gctcgttgtc gtaaacatgc tcgccgccga acagcgcgtt aatggtttcg tggatgccga
    23521 tgtaacccag cgaaatagac gcacgaccgt ttttgaagat ttcagaaaca tcatcgtcag
    23581 cattcagacg cacgccacaa gcaccttcca tatagaggat cggggccacg cgcgctttca
    23641 cgccttcgag acgagcgata cgggtcatca gcgccttacg tgccagcacc agacgttcat
    23701 ccagcagctt ccagaaggtg gcttcatcgc cttttgcttc cagagcaata cgcggcaggt
    23761 tcaggctgat cacgccgagg ttgttacgac catcgtggat ctgctcgccg ttttcatttt
    23821 cccacacgcc gaggaagctg cggcagccca tcggggtttt aaacgaaccg gtgactttca
    23881 ctacctgatc gtagttcagg atatccggat acatgcgctt gcttgcgcac tccagcgcca
    23941 gctgtttgat gtcgtagttc ggatcgcctt ttttatggtt caggccatcg cgaatcgcaa
    24001 acaccagttt cgggaacacc gcagttttac ggtttttacc gaggcctgcg atacggttac
    24061 gcaggataga ttcctgaatc aggcgcgact cccagctggt tcccagacca aaaccaaagg
    24121 ttacaaacgg cgtctgaccg ttggcggtgt gcagggtgtt tacttcgtac tccagcgact
    24181 ggaaggcatc gtagcactct ttaatggtac gagagttagc gtagccttcg gcgtccggga
    24241 tgttccactc ttctgcggtc ttgcgatgtt tgttgtagct ggcggtgaca aacggtgcca
    24301 gcacttcatc gatacggtta atggtggtgc cgccataaat atggctggcg acctgagcaa
    24361 taatctgcgc agttacagcg gttgccgtag agatcgactt cggcggttca atctcggcgt
    24421 tccccatctt aaagccctgg gtcagcatgc ctttcaggtc gatcaacatg cagttgaaca
    24481 tcgggaagaa cggcgagtaa tcgagatcgt gatagtgaat atcgccacgc tcatgtgcct
    24541 gcaccacgtc acgcggcagc aggtgctgac gtgcatagtg tttagccacg atcccggcca
    24601 gcaggtcgcg ctgggttgga atcaccttgc tgtctttgtt ggcgttttcg ttgagtaacg
    24661 aggcgttggt ctgctcgacc agaccacgga tctcctgatt caggcgaccg cgtttttcac
    24721 gttcaatgtc gcgatcgtga cggtactcga tgtaagcacg agccagttgt ttgtatggac
    24781 ctgacatcag ctggttttca acagcggtct ggatctcatt gatatccacc tggttgcggc
    24841 cctgcatctg ctcgctgaca actgcagcaa cagtggcgca ataatcggca tcatcgactt
    24901 ccgctgcttt agctgcacgc agaatcgctt ctttgatgcg ctctgattta aacggcactt
    24961 tgcagccgtc tcgtttcatc acatgcggtg tcatgatcgc tccatatttt taagaacagg
    25021 ttatccacag aaattgggaa aggcgttttc cggttttttc gtttcgtttt ccggtgcttt
    25081 ccgcaatcgc catccgctta tccacaacgc cgcacctttt tcgtggggag ctgtagtagc
    25141 aattatagtc gattaataca acatattggg ttgggacgca ttttaaagtc tatatatagt
    25201 gctttgcatc aaggatgttt gaactttttt tgatgtagct caaagtaaaa agcagagcgt
    25261 acggatgacg ggcgctacag cgttatgtaa attttttaat gaatttgctg gttaaaaaat
    25321 caacaaaaac aacatactga cagacaaaac cccggaatga ccggcattac cggggcttag
    25381 ggaagattta cttctgtaac caccagacag cctcaaaagg ccgtaaagtc atggcacagg
    25441 gttgtggtga ggcttcttcg tagttatgca tcacaagctg ccagttgccg cgcatttgct
    25501 ctggctgcca gggttggatc tcacggctaa ggttggcaat gaccagcaag gtttgcccct
    25561 tccattcacg gcgatagcac cacaatacag ggctgtttgg cagcagatcc tggtaattgc
    25621 cccatgtcag aacggcttcc tgcttacgca gtgcgattaa cttttggtag gtgtaaaaca
    25681 ccgaggactc atcggccagc gcggcttcta cgttgatttg ttgatagtta tcgcccaggc
    25741 caatccacgg ttcgccagcc gtaaacccgg cattatcgcc gttgctccat tgcatgggcg
    25801 tgcggctgtt gtcacgggat ttactggcga ggattgccaa taactcgtcg gcatcacgcc
    25861 catcgttgcg cagctcggca aacatattga ggctctctac gtcgcgatag tcagtaatgc
    25921 gcgtgaaatg cgggttggtc atgccaatct cttcgccctg gtagatatac ggcgttccct
    25981 gcatgccatg cagcaccatc gccagcattt ttgccgcagg cacgcggtat tcaccttcat
    26041 caccaaagcg agaaacaatg cgcggctgat cgtggttaca ccagaacaag gcattccatg
    26101 ctacgttgtg cattccttgt tgccagtggc ggaacaatgt tttcaacgcc acaaagtcag
    26161 gtttagccag cgtccatttt tcaccaccgg gataatcgac cttcaggtga tgaaaattaa
    26221 aggtcatcga caattcactg ccagtcagtg ccgcgtatcg ctggcaatgc tcaaggctgg
    26281 tggaggacat ttcacctacg gtcattaacc cgcgtggcgt aaacacatcg cggttcatct
    26341 cgtgcaaaaa ctcgtgtgct cgtggcccgt cggtgtagaa gcgacgcccg tcgccatcca
    26401 ggtcatcagg gaaacgcggg tctttggaga tcagattcac cacatccagg cgcaacccgt
    26461 cgaccccacg atcggcccag aactcacaga cttttttcag ctctgcgcgt accgctggat
    26521 tctcccagtt gagatccgcc tgttctggtg caaagagatg caaatagtac tgttcgcttt
    26581 ccgcatgcca gcgccacgca ctaccgccaa attttgaacg ccagttgttc ggtggcgttt
    26641 ctggttctcc atcgcgccag atataaaact ggcggtaagg gctttctttg ttcagcgcct
    26701 cgcgaaacca ggcatgttgg gtagaggtat ggttaaacac catatcgaga atgatacgaa
    26761 tcccgcgcga ttttgcctgc gtcaccagtt cgtcaaaatc gtccagcgtg ccgtaggtgg
    26821 gatcaatcgc cgtatagttc gctacgtcgt aaccgttatc gacctgggga gagacataaa
    26881 agggggttag ccagatggca tcaacgccca gtttatgcag atagtccagg cgttggataa
    26941 cgccacgtaa atcgccggtg ccgctacccg tggtgtcctg aaaactcttt ggataaatct
    27001 ggtagataac gccgttttgc caccagtggg gaagattagt cattacgtta ttcctgcaaa
    27061 tgcgaggggg cgcaattgcg ccccgaagaa aattaaacaa tgtccagcgt gcccaggcgg
    27121 tatttccgct gatagataaa cgaggtgagt acaatcggga tgatgatggc gatagccatt
    27181 gccagcgcaa acacctgcca gtagctcggt tgaatcgaga gaattcccgg caggccgcct
    27241 acgccgatgc cattcgccat aacgccgttc aggccgcata gcaatcctgc cagaccagaa
    27301 ccaatcatcg cgcacagcat cgggaagcga tatttcaggt tgatgccgta cattgcaggc
    27361 tcagtgaccc caagccaggc ggagatcgcc gcaggcacgg agatctcgcg ttcattgtgt
    27421 ttgcggctgg aaatgataat gcctatcacg gcggagccct gagcgatatt cgacagcgca
    27481 atcagcggcc acactggcgt accacccatg ctttgaatca tctgcaaatc aatagcaagc
    27541 gtggtctggt gtaccccggt gatcaccagc ggggcgtaca ggaagccaaa caatgccgcg
    27601 ccaatcggag caaagctgcc ggtcatcagg tgacgtaccg caaaggcaac gccatcgcca
    27661 atcatgcgac caaacggacc aatcagcgca tgggcgagga acaccgcgag gatcagcgaa
    27721 cagacgggca ccaccaccag atagaggtaa tctggcacga tgcgtttaag gcgagtttca
    27781 ataacgccca gcgccagccc ggctaatagt gccgggatca cctgcgcctg atagcccact
    27841 ttggcgatgc tgaacatgcc aaagtcccac acttccggca gctgctgccc gagcagataa
    27901 gcgttcatca gctgggggga aaccagtgtc acgccaagca cgataccaag gatcggcgtg
    27961 ccgcccattt ttttcaccgc tgaccagcaa atcccgaccg gcaggtagaa gaagatcgct
    28021 tcaccgatca accacagaaa atcgtagatc gtttgcaggg aagggtacat ttgcgccagc
    28081 gtttgaccgt tgctcatggg caaatcgccg atcacattgc gaaaaccgag gatcaaaccg
    28141 ccgctaatca acgcgggcag caacgggaag aagatctctg cgaaatgaga gatcaactgc
    28201 tcatgccatt tcatattctg ccgggcggct ttttttacct gctctttatc aacctgcgcc
    28261 tgtccggttg acgctatcag tgcttgatag taatcaccca cgttggtgcc aatcaccacc
    28321 tgaaattgcc cggcattggt gaaacagcct ttcaccatgg ggagttgctc aatttctttc
    28381 ggtctggcat tggccggttg gttgaggaca aagcgtaggc gagtaataca gtggctcacc
    28441 gtcgcaatat tgccgcgccc gccgaccagt tcaatcaacc gatcgatatc cgtttggttt
    28501 attttgctca tcataaagcc ccatggcaga tgacattttt ggttggctgc agaatatatt
    28561 gcgcggaaaa atttaaaaac gggaacgttc ccgaaacgca gcgaagatca caatttatcg
    28621 ttcaggaaac gatcaggaca gggtggcggg gatgatgatt tgttgcggtt cgctgcgtcc
    28681 ggttacctgt gcgatcaact ggcaggccgc ctggcgtcca gcttcggcgt aaccgggatc
    28741 tacggtgacg atctccggat ggaggaattt cattaacggc gtattaccga cgctcgccag
    28801 ttgcaaggtg tcgatgcgtt gctcttgcag gtatttactt gcgccaagtg ccagcgtgtc
    28861 ggttgcgcac agtaaggcgg tagtttcagg cgtaatcact tttgctacgt tctcatagcc
    28921 ttgcttcata gcaaggcctg gcagagcggc aacgggatgc agtttatgcg ctttgcagaa
    28981 cgccaggtag gcttcgtgac gtcgcttacc ggtcgtcacg tcactgtgcg gcacgccgag
    29041 ataactgata ttacgatgcc cctggtcata cagccgttgc atcaggatct tgattgcccc
    29101 ttcgtcgtca taacagaccg aagcaaagcc ttttgcgtca cgcgccagca gaaccagcga
    29161 tgactgccag tgggctaaca tttcttctgt tatgccagta aaaccgaaca gcactacgcc
    29221 gtcgatatta cgccgtttca gcacccccaa atgttcggca actaattgcg gggaaaactg
    29281 actttccatc atgattgggt cgtaaccttg ttcatagaac gctggcagca tggtttgaac
    29341 ggcgagattt tctgacaacg aatccagacg ggtaacaatg atggcgacca ctttatcgct
    29401 ttgcccacgc atagcgcgcg cagagcggga aggggaaaat ccatgctgat tcatcactgc
    29461 ttcaacacgc tcgcgggtgc gctggctcac gccgctttcg ttattcagca cccgggaaac
    29521 tgtagatttc cccacgccgc ttaagcgcgc gatgtctttg atggtcagcc gattttgcat
    29581 cctgttgtcc tgtaacgtgt tgtttaatta tttgagccta actttacccg tgcattcagc
    29641 aatgggtaaa gtctggttta tcgttggttt agttgccagc aggtattata tcgccataga
    29701 tgctacgaat attattggat tctccttatt atttgcggcg cttttttcac ttaccggagg
    29761 ttatatggaa cctgatccca cgcctctccc tcgacggaga ttaaaacttt tccggtaagc
    29821 ccgtcttttc acggcgttac cggatgcgta aggccgtgac gttttaacgt ccctgctcag
    29881 ctttattacc ttcaggtaag gcttcgccac gcctgaagac atttctgtac tgtttcagac
    29941 agtgcggagg gactccttat gtttaaagaa atttttaccc ggctcattcg ccatttacct
    30001 tcccgtctgg ttcatcgtga tccattgcct ggcgcgcagc agacagtgaa tacggtggtc
    30061 ccgccgtcct taagtgcgca ttgcctgaaa atggcggtga tgcccgaaga agaattgtgg
    30121 aaaacgttcg acacccatcc ggaagggtta aatcaggcgg aagtggaatc tgcccgcgaa
    30181 caacatggtg aaaataaatt acccgcacaa caaccgtcgc cgtggtgggt acatttatgg
    30241 gtctgctatc gcaacccctt taatatttta ctcaccattc tcggcgctat ttcttacgcc
    30301 acggaagatt tatttgctgc gggcgttatc gcgctaatgg tcgctatttc tacgttgctg
    30361 aactttattc aggaagcacg ttccactaaa gcggcagatg ccctgaaagc gatggtcagc
    30421 aatactgcga cggtgctgcg cgtaattaac gacaaaggcg aaaatggctg gctggagatc
    30481 ccgatcgacc agctggtgcc cggcgatatt ataaaactgg cggcgggaga tatgatcccg
    30541 gcagatttac gtatcttgca ggcgcgggat ctgttcgtcg ctcaggcgtc gttaaccggt
    30601 gagtctctgc ccgtagaaaa agccgctacc actcgccagc cggagcacag caatccgctg
    30661 gagtgcgaca cgctgtgttt tatgggcacc accgtggtga gcggcacggc acaagcaatg
    30721 gtgattgcta caggtgccaa tacctggttt ggtcaactgg cggggcgtgt tagtgagcag
    30781 gaaagcgagc cgaatgcctt tcagcaaggg atcagccgcg tcagtatgct gctgattcgc
    30841 tttatgctgg tgatggcacc ggtggtgctg ttaatcaatg gctacaccaa aggcgactgg
    30901 tgggaagcgg cgctgtttgc gctttcggtc gcggtaggcc taacgccgga aatgttgccg
    30961 atgattgtta cctcgacgct ggcgcgcggc gcagtaaagc tgtcgaaaca gaaagtgatc
    31021 gtcaaacatc tggatgctat tcagaacttt ggcgcaatgg atattctgtg cactgataaa
    31081 accggcaccc tgacgcagga taaaattgtg ctggagaatc ataccgatat ctccggtaaa
    31141 accagcgaac gcgtgctgca tagcgcgtgg ttgaacagtc attatcagac cggacttaaa
    31201 aacctgctcg atacagcggt gctcgaaggt acggatgaag agtcagcgcg ctcgctggcc
    31261 agtcgttggc aaaaaatcga tgagattccg tttgatttcg agcgtcgccg gatgtcggtg
    31321 gtagtggcag aaaataccga gcaccatcag ctggtttgca aaggcgcatt gcaggaaatc
    31381 ctcaatgtgt gttcgcaggt gcgtcacaat ggcgagattg tgccgctcga tgacatcatg
    31441 ctgcgtaaga ttaagcgggt tactgatacg ctgaatcgtc aggggctgcg cgtggttgcg
    31501 gtggcgacga aatacctgcc agcgcgtgaa ggagattacc agcgggcgga tgaatccgac
    31561 ctgatccttg aaggatatat tgcttttctt gatccgccaa aagagacaac cgctccggca
    31621 ctgaaggcat taaaagcgag tggaattacc gtaaaaatcc ttactggcga cagtgagtta
    31681 gttgcagcga aagtgtgcca tgaagtgggg ctggacgcgg gagaggtggt cattggtagt
    31741 gatattgaaa cgctatctga cgacgaactg gcaaatcttg cacagcgcac cacgctgttt
    31801 gcccgcctga cgccgatgca taaagaacgc attgtgacct tactgaagcg cgaagggcat
    31861 gtggttggct ttatgggcga tggtattaat gatgcgcccg ccttacgcgc ggcggatatc
    31921 ggcatttctg tggacggcgc ggtagatatt gcccgtgaag cggctgatat catcctgctg
    31981 gaaaaaagcc tgatggtgct ggaagagggg gttattgagg gacgtcgcac tttcgccaac
    32041 atgctgaaat acatcaaaat gacggcgagt tctaacttcg gtaatgtgtt cagcgtgctg
    32101 gtggcgagtg ctttcttgcc cttcctgccg atgttgccgt tacacttgct tattcagaac
    32161 ctgctgtacg atgtgtcaca ggtggcgatc ccgtttgata atgtcgacga cgagcaaatt
    32221 caaaagccgc agcgttggaa tccggcggat ctggggcgct ttatgatctt cttcggaccg
    32281 atcagctcga tcttcgatat tctgacgttt tgcctgatgt ggtgggtatt ccatgccaac
    32341 acgccggaaa cgcaaacgct gttccagtcg ggatggtttg tggtgggctt actgtcgcaa
    32401 acgctgattg tgcatatgat ccgcacccgc cgtgtgccgt ttattcagag ctgtgcatcg
    32461 tggccgttaa tgatcatgac cgtgatcgtg atgattgtcg gtatcgcatt gccgttttca
    32521 ccgctggcca gttatctgca gctgcaggcg ctgccgttaa gctatttccc gtggctggtt
    32581 gcgattctgg cagggtatat gacattaacc cagttggtga aagggttcta tagccgtcgt
    32641 tacggctggc aataaagaat caactgggca tggtgacccg gattcagatt gcagacaaag
    32701 tgagttttgt tcatgccgga tgcggcgtaa atgccttatc cggccaacaa attcaataaa
    32761 ttgcaggaat cacgtaggcc tgataagcgt agcgcatcag gcgattttgc gtttgtcatc
    32821 agacttaatc cgggcatgat agcccggatt tccatcaaga ttagcgacga acagcgatcg
    32881 cttcgatctc aatcttcacg tctttcggca gacgggctac ttcaacgcaa gaacgtgccg
    32941 ggaaggtggc gttgtgttcg gtgaagaagg cttcgtaagt ggcgtttacg gttgcgaagt
    33001 cgttcagatc ttttacaaac acggtagttt taacgatgtc gcccactttc aggccagcgg
    33061 cttcgacgat cgcttttacg ttatccagcg actgacgtgc ctgtgcagcg acgtctgccg
    33121 gtacttcgcc cgttttcgga tttaccggga tctgaccgga ggtgatgatc atattgccca
    33181 gatcaacgcc ctgtacgtaa ggaccgatag ctgccggtgc attttccgtc gcgatagttt
    33241 tgctcatgat ttctccttta ttacagcggt aaaaagtctg gctcattata gggagccaga
    33301 cttttattac caaccgcaat taattggcca gcaccacatt atgggaaaac tctttttcac
    33361 agtatttgca tttgagcgcg atatcattgg cgcgttttcg cacggcaaag ctggaagaaa
    33421 ccggttcggc atggctgata cagttgctgt tcgggcagac cagcacattg tcgatgcgct
    33481 ccggcagact tgggcgcgat ttacccacca cttcatagtt gtcgatacgg ttaaccgtgg
    33541 cttgcggcgc atacaatgcc agttgatcta cttgatcttc actcaaaaag gtattttcga
    33601 ttttgattag atctttgcgg cccatctcgc cagaaggcag gttcagaccg atggtaatgc
    33661 gctgatccgt ttcggtcagc ttgaacagac tcaacagctt aaaaccgatc tgggcgggga
    33721 tatggtcaat taccgtgccg cgtttaatag cttcaacctg caatttatta tcgtgtgtca
    33781 tctctatttc cccttacagt accagatcgc gattcagaac cagtgccagt aacgcctggc
    33841 gagcgaaaat cccattgcct gcctgctgga agtaccaggc gtgtggcgtt ttatcaacat
    33901 ccgtcgcaat ctcatcaaca cgcggcagcg gatgcagcac tttcatattg gctttggcgt
    33961 tgtggagatc gctggcgcga agaacaaact gcgctttcac gttggcgtac tcggacgggt
    34021 ccagacgctc tttttgcacg cgggtcatgt acaggatgtc aacttccgcc atcacttctt
    34081 caatagagct gtgcagactc catgcgatcc ctttttcatc gagcatatcc agaatgtatt
    34141 gcggcattgc cagcgcgtcc ggcgcgatga agtaaaaacg gttgccgtcg aacttcgcta
    34201 acgcctgagt cagggagtga acggtgcggc catatttcag gtcaccaacc attgcgacgt
    34261 ggagattgtc cagacgcccc tgggtttcct gaatagtgaa taagtccagc aaggtttgcg
    34321 tcggatgttg gttggagcca tcaccggcat tcagtaccgg tacattgccg gaaaactcgg
    34381 tggccaggcg cgccgcacct tcctgcggat gacgcatcac tatcgcatcg acgtaagtgc
    34441 tgataaccga aatggtatcg gccagcgttt cgcccttttt acccagtgat gtattggcgc
    34501 tgtcggagaa gcccaccacg ctggcaccca ggcggtgcat ggaagtttcg aaagagaggc
    34561 gggtacgggt agaggcttcg aagaagcagc tggcaatgac tttgtgtttc aacagctctg
    34621 gttgcgggtt tgctttcagt tttgccgctg ttgccagcac cagattaagg tcatcgcgac
    34681 taaggtcgtt tatggaaatg atatgtttct gatatagcgg attagccatc ttttatctcc
    34741 tgacgcctgg gcaaaaaaaa gcccctcgat tgaggggctg ggaatgggtg atcaacggga
    34801 agaaaaacgg caggccagcg tcttttttca gacgcggtaa gacaaaatgt cgaacacact
    34861 gaaccataca tcctcccggc aaattgtccg gcattatact aatcgtcaga agcggtgcaa
    34921 gcatttgatg caatattttg tcagcgcaaa cggtttattt gaatttaaag tcaaaatata
    34981 tgcattttta tttactgtga ttctggaggg gaactgtcgt tcggtatcaa taattgcaga
    35041 catttcctgc ttttttcttt ttcactttca cgcaatcaga tatgcatttt attccattca
    35101 tccgacttat agggcggagt gtgaaaacga acggctaaca ctattgctta cagctcaggg
    35161 atgcgcgcta tcactttaat ttcaaaatca aagcctgcca gccatgtaac acccaccgcc
    35221 gtccagtttg gataaggtgg ggcgctaaat atttcatttt tcaccgtcat gatgtcttcg
    35281 aattgttttt ctggatcggt atgaaagctc gtaatatcaa tgatattgtc aaaagtgcat
    35341 cccgcagcag ccagggtcgc atgcaaatta tcaaatgcca gtctgacttg ttgctgaaaa
    35401 tcgggttctg gtgatccgtc ctctcgactt cctacttgcc cggaaacaaa cagcaaatcg
    35461 ccggaacgaa tagccgcaga ataacgatgt tctgcataca gtgaatgtcg gccagcaggg
    35521 aaaacagcgg ttctttctac catttggtta tcctcaagat ttacgacatg aacagaagat
    35581 ttatatttac cgggagccgc ttttagcgga cgacgtgagt aaacaaaacc cagacatcat
    35641 ggataatggc tgggcttaat tgagcgtagt cggttatgcg ccaaacgcgc catcaatggt
    35701 atgcatcgcg ccggtaacaa aactggcttc tggccctgct aaccatgcga ccataccagc
    35761 gacctcttcc ggttgcccat gtcttttgat ggccataaaa ccatgcaaca tatcgcgcat
    35821 tggcccgttg gcgggattag cgtcggtatc aattggccct ggctggacga cgttgatggt
    35881 gatcccacgc ggtccaaaat cacgggccag cccgcgcgcc atgccttgta gggcagattt
    35941 actggcggca taagcagcca tgcctgcaac aggcatacga tcgccattca cggagccgat
    36001 gattaagatg cgcccgcctt cgggcatctg ccgggcggct tcaacagagg catggtaagg
    36061 agcatgaata ttgattttga aaaggtgatc aatatcgtcg gcatttaatt ccagggcatc
    36121 gccaaagacg ccaatacctg catttaccac caggatatcc aatgcgccgc tcttacgaac
    36181 gacatcaata acagcgtctc tgtcagcact atctgtgaat actgctgtcg ctccagtctc
    36241 ttgtgccagg cgttcagcgg catctttcga ccccgcatag gtgaatcgta cattggcccc
    36301 atcggtgacg aaacgacgta cgatagcggc accgattcca cggctaccac cgaggatgag
    36361 aactgtctta cctgtaaaag cgcccataag gactccttga tttattatgt aacatgcatt
    36421 acaaaactgt tttaactttc tgtcaacaag ttttgtaatg gtcactaaaa aacaatctcg
    36481 cgttccaggt cgtcccagac gtttcgctcc tgagcaggca gtctctgcgg caaaagtgct
    36541 ttttcaccaa aaaggtttcg atgctgtcag tgttgctgaa gttactgatt atcttggtat
    36601 taaccccccg agcctctacg cggcttttgg cagtaaggct gggttattta gccgtgtact
    36661 caatgaatat gtcggtacgg aagctattcc gcttgtcgat attcttcgtg atgatcgtcc
    36721 ggtaggggag tgcctggctg aggtattaaa agaagcggcg cgcagatata gccaaaacgg
    36781 cggctgcgct ggctgtatgg ttcttgaagg cattcatagt catgatccac aagcgcgtga
    36841 tattgccgtt caatattatc acgccgcaga aacggccatt tatgactata tcgccaggcg
    36901 gcatccacaa agcgcacaat gtgtgactga ttttatgagt accgtgatgt cagggctttc
    36961 tgcgaaggca cgagaggggc actccctcga acaactctgt gcaacagctg cactggcggg
    37021 gggagcgata aaaactattc tcaaggagtg atgcgggcct tgacccgaaa ggcgggaacg
    37081 cgcctgccga taagttgtga taagacaata attcacgcgt taaggctagc ggaattgatc
    37141 atcttttcgt ataacgctag aaatgaaacg ttgttttaat taaggagtgg aaaagatgat
    37201 catcggaaat attcataatc ttcagccgtg gctaccccag gagttacgcc aggcgattga
    37261 gcatatcaaa gcacacgtta cggtagaaac gccaaagggc aagcacgata tcgaaggcaa
    37321 tcgtctgttt tatcttatct cggaagatat gaccgagccg tacgaagctc gccgtgcgga
    37381 gtaccatgcc cgctatctcg acattcagat tgtgttaaaa ggtcaggaag gcatgacctt
    37441 cagcacacaa ccggcaggta cgccggatac cgactggtta gctgataaag acatcgcatt
    37501 tttgccggaa ggcgttgatg agaaaacagt tatccttaat gaaggtgatt ttgttgtgtt
    37561 ttatccgggg gaagtgcata aaccgctgtg cgcagtgggc gcgccagccc aggttcgcaa
    37621 agcagtagtg aagatgctga tggcgtgatg acttttcgcc gtaaataact ccaggtttac
    37681 ggcgagtttg tgaaaagagc gttttttgat atttttttgt gagtaaaatt tgtaatgctt
    37741 agacgttctt attaactcaa ggagttcgtc atgagcaaaa tatcagattt gaattattct
    37801 caacacatta catcagccga taattttaaa ccaaaaaatg aagtcttaaa tacctggtgt
    37861 gtgggaatga atgattttgc ccgtattgcc ggggggcagg atagcagaag gaatattctt
    37921 tctcctggag catttttgga gtttttggca aaaatattta ccctgggtta tgtggatttt
    37981 agcaaacgct ccaacgaagc gggtagaaat atgatggctc atattgagtc ctcatcttat
    38041 atcaaaaata atgatggcag tgaaataatg aagtttgtta tgaataatcc tgaaggtgag
    38101 cgagcggatt catctaaggt gattatagaa atttcacttt ccactactac tatggggact
    38161 cgtcaaggac atacagccat tatatttcca caatctgatt ttttgagttt tcgttatgaa
    38221 gggaagtctt ttgaaagaaa agatgatagt tcattacacc tgattactaa caaggttctg
    38281 gcgtgttacc aacgcgaagc taacaaggaa atagcgcgtc tattaaataa tca
//
LOCUS       DAZFKF010000040        38032 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFKF010000040 DAZFKF010000000
VERSION     DAZFKF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.040"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            81..1436
                     /locus_tag="ACHKA1_002474"
     CDS             81..1436
                     /locus_tag="ACHKA1_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HFO5680113.1"
                     /translation="MARFQFKSRKNNGFIFLISFMVMGEATIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPDVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDINLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMVSAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
     gene            1539..22316
                     /locus_tag="ACHKA1_002475"
     CDS             1539..22316
                     /locus_tag="ACHKA1_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO5680114.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMQDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHITFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTTVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDTTGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGEVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTA
                     DNIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVDAL
                     ADGNATVTASVNDVAGNPTSVSRVALVDATPPVVTINPVATDNVINTPEHIQAQIISG
                     TVTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDKAG
                     NTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNG
                     QNYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSSIGNSTTASHNVLVDSALPG
                     VTINPVATDDIINAAEAGVAQTISGQVTGAAVGDAVTVTLGGNNYTTTVQAGLSWSVD
                     VPADAIQALGNGDLTVNASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVVTGSSLGLAEGTPLTVTINDVEYTTAVQADGSWSVGITAAQVSAWPAGAVNI
                     AVSGESSAGNPISITHPVTVDLTPAAITINTIATDDVINAAEKGANLTLSGTTTNVEA
                     GQTVTVTFGGKNYTASVAGDGSWTATVPAADLAALPEGSASAQASVSNINGNSASAVH
                     SYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTVTLNSPTVQTYQ
                     ATVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTIN
                     TIAGDDIINATEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAA
                     DVTALGSGPQTVTATVTDAAGNSDSETHTVTVNLTAPTIGINTIATDDVINATEKGAD
                     LQISGTSNQPAGTTITLTLNGQNYTATTDASGNWSTTVPASAVGALGEASYTVTANVT
                     DSAGNSNSASHNVQVNTALPGVTLNPVASDDIINAAESGVAQTISGQVTGAAAGDTVT
                     VTLGGKTYTATVQGNLSWSVDVPAADIQAIGNGELTVNAWVTNGVGNTGSGSRDITID
                     ANLPGLRVDTVAGDDVVNSIEHGQALVITGSSSGLAAGAALTVVINNVTYGATVLADG
                     TWSLGVPAANVGNWPAGTVNITVSGATSAGNPVTITHPVTVDLAAVAISINTVSGDDV
                     INAAEKGADLTLSGSTSGVEPGQTVTVTFDGKTYTATVAGDGSWTTTVPAADLAALRD
                     GDATVQASVSTINGNTASATHVYSVDATTPTLTINTIATDDILNAAEAGNPLTISGSS
                     TAEAGQTVTVTLNGVTYTGTVQADGSWSVSVPTADLSNLTASQYTVSASVNDKAGNPV
                     SATHGLAVDLTVPVLTINTVSGDDIINATEHGQALVISGSSTGGEAGDVITVTLNSKT
                     YTTTLDASGNWSVGVPLSDVTALGSGPQNITATITDAAGNSDDASRTVTVNLAAPTIG
                     INTIATDDVINATEKGADLQITGTSNQPAGTTITVTLNGQNYTATTDASGNWSATVPA
                     SAVSALGEANYTVTANVTDKAGNSNSASHNVLVNSALPAVTINAVATDDIINADESDN
                     AQTISGQVTGAAAGDTVTVTLGGNTYTATVQANLSWSISVPAADIQALGNGDLTINAL
                     VTNGVGNTGSGSRDITIDANLPGLRVDTVAGDDVINSIEHNQALVITGSSTGLTAGTA
                     LTVEINNVTYAATVLADGTWSLGVPAVDVSNWPAGTVNITVSGTNSAGTTSTITHPVT
                     VDLAAVAITINTLSGDDVINAVEKGETLVVSGSTSGVEAGQTVTVTFGGKNYTTTVEA
                     NGSWTVNVPPADLAALPDGAGNVQASVSNINGNNAQADRAYSVDATAPLVTINTIASD
                     DTLNVSEAGAGITISGTTTAQAGQTLTVTLNNNTYQTTVQADGTWSVNVPAADLSGLT
                     ASSYTVTATVSDKAGNPASADHALAVDVTAPDLTINTVAGDDIINAIEHGQALVVSGT
                     STGAAAGDVVTVNLNGKNYTTTLDASGNWSVGIPAADVTALATGSQTITASLSDRAGN
                     SDSTTHNVTVDLSGPTLTISIVSGDDIINNAEKTQDLIISGGSSGLATGTTVTVMLNG
                     LAYSATTDGSGNWSVTVPASAVGALGEAVYQISASATDSAGNSGITTHTVNVESLLPG
                     VIINTVAGDDIINAAEIGVNQTISGQVTGTAAAGDTVTVTLGGNQYVATVQPDLSWSV
                     SVPANVLQALGNGELTISASVTNSANNTGTTTHDIVIDANLPGLRVDTVAGDDVINSI
                     EHTQALVITGSSSGLAAGAALTVVINSVTYGATVLADGSWSVGVPAADVTNWPAGTVN
                     IAVSGTNTAGTTTSITHPVTVDLAAVAITINPLSTDDVINAAEKGSDLQLSGTTSDVE
                     AGQTITVIFGGKSYTTTVAADNTWGLTIPAADLAALPDGAANVQASVSSVAGNSAQAT
                     HVYSVDATAPSVTINTIAADDILNAAEAGSALIISGTSTTEAGQTVTVTLNGVNYSGN
                     VQADGSWSVSVPTGDLANLTASSYTVNASVSDKAGNPASATHNLTVDLAAPVVTINTV
                     AGDDIINATEHGQAQIISGSATGATTGNTVSVTIGTTTYTTVLDANGNWSIGVPASVI
                     SALAQGDVNISATVTDSAGNSGTASHVVSVALGAPVLAINTIAVDDIINATEKGADLA
                     ISGTSDQPAGTQITVTLNGQNYTTTADASGNWSVTVPASAVGTLGEATYTVTASATDA
                     DGNSGSASHNVQVNTALPGVTINVVATDDIINAAEAGVDQTISGQVTGAAAGDTVTVT
                     LGGATYTATVQANLSWSVDVPASALQALGNGELTISASVTNSVGNTGNGTREITIDAN
                     LPGLRVDTVAGDDVVNIIEHGQALVITGSSSDLAAGSNVTLTINGQTYVAAVLADGSW
                     SVGVPAADVSAWPAGTVTITASGNTTAGNPVSVTHPVTVDLTAVAVSINAITVDDVIN
                     AAEKGAALTLSGSTSGVEAGQTVTVNFGGKTYTASVATNGSWSTTVPAADMAALRDGD
                     ASAQASVSNVNGNSATTTHAYSVDASAPTVTINTIAGDDILNAAEAGAALTITGSSTA
                     EAGQTVTVTLNGENYTGTVQTDGSWSVSVPQADVSALTASNYTVSAAVNDKAGNPASV
                     NHNLTVDTSVPVVTINTVAGDDVINATEHAQAQIISGSATGAATGSTVTVTIGTNTFT
                     TVLDASGNWSVGVPASVVSALANGTVTINASVTDVAGNSGSATHQVTVNTGLPTITFN
                     AISGDNVLNADEKGQPLTISGGSTGLATGAQVTVTLNGHNYSATTDASGNWTLTVPVS
                     DLAALGQANYTVSASATSAAGNTASSQANLLVDSGLPDVTINTVAGDDIINAAEAGAD
                     QTISGVVTRAAAGDTVTVTLGGNTYTATVQSNLSWSVSVPTADLQALGNGDLTITASV
                     TNANGNTGSGTRDITIDANLPGLRVDTVAGDDIVNSIEHGQALVITGGSSGLNAGAVL
                     TVTINSVAYSATVQADGSWSVGIPAANVSAWPAGPLTVEVAGQSSADNPVSVSHPFTV
                     DLTAVAISINTVASDDVINAAEKGTNLTLSGSTSGIESGQTVTVTFGGKTYTASVAAN
                     GSWSKTVPVADLATLPDGAANVQASVSSASGNSASATHAYSVDASAPTLTINTIASDD
                     ILNATEAGSPLTISGSSTAETGQTVTVTLNGATYTGTVQADGGWSVSVPTSALGALNA
                     SNYTVSATVNDKAGNPGSASHNLAVDTTAPVLTINTVAGDDIINDAEHAQALVISGTS
                     TGGEAGDVVSVVLNGKTYTTTLDASGNWSVGVPAADVAALGSGAQTITASVSDRAGNS
                     DDASRTVTVSLSAPVISINTIAGDDVINATEKGSDLALSGTSDQPAGTAITVTLNGQN
                     YSATTDASGNWSVTVPASAVSALGEATYSVTASVTNAQGNSSTASHNVQVNTALPGIT
                     INPVATDDIINASEAGSAQTISGQVTGAAAGSTVTVELGGKTYTATVQPDLSWNVSVP
                     AADWQALGNGELTVNASVTNAVGNTGSGTRDITIDASLPGLRVDTVAGDDVVNIIEHS
                     QAQVITGSSSGFAAGTALTVVINNQTYAATVLVNGTWSVGVPAADVSNWPAGTLNITV
                     SGANSAGTQTSITHPLTVDLTTVAISINAITPDDVINAAEKGAALTLSGSTSGVEAGQ
                     AVTVTFGGKTYTTTVAANGSWSTTVPAADLAALRDGDASAQVRVTNVNGNSATATHEY
                     SVDSAAPTVTINTIASDNIINASEAAAGVTVSGTSTAQTGQTLTVTLNGTNYQTTVQA
                     DGSWSLTLPASDLTALANNGYTLTATVSDLAGNPGSASKGVTVDTTAPVISFNTVAGD
                     DVINNVEHTQAQIISGTATGAVAGDRLVVTIAGQQYVTSTDASGNWSVGVPASVISGL
                     ADGTVTISATITDSAGNSSTQTHNVQVNTAAVSLSVSTISGDNIINAAEAGSALTLSG
                     TGTNFATGTVVTVLLNGKGYSATIQSNGSWSVNVPAADVAALSDGTSYTVSASAQDSA
                     GNSATASRSVAVDLTAPVISINTVSTDDRLNAAEQQQPLTLNGSTSAEVGQTVTVTFG
                     GKTYTATVAANGTWALNVPAADLAALGQGAQTITASVNDRAGNPGQATHALTVDTVAP
                     TVTIATVAGDDIINNAEQLAGQTISGTTTAEVGQTVTVTFNGQSWSATVGSGGSWSVF
                     IPAQQFAGLSDGSYTISATVSDQAGNPGSASRGVTLNGDVPTVTINTFAGDDVVNAAE
                     HGSSLVISGTTTAPVGQTLTLTLNGKTYTTTVQTGGSWSYTLGSADVTALADGNAYVI
                     NASVSNAIGNTGSSNHTITVDLSAPAMGINIDSLQADTGLSASDFITSVSPVVVNGSL
                     TAALASNETAQISIDGGTTWTTLTVTGTTWRYNDSRTLTDGNYLYQVRVIDAAGNVGA
                     TDSQNVVIDTTAPDPAVKTIAISAITTDTGLITNDFVTSDTTLAVSGTLGATLSAGEF
                     AQISIDGGTTWQNLSVSGLTWSYLDGRTLTDGNYNYQVRVIDTAGNIGATASQIVTVD
                     TTAPLASKTIAIASISDDTGLSSSDFVTRDTTLTVRGTLGAALAADERAQISLDGGVT
                     WTTLTVIGTSWSYADGRTLTDGTWNYTVRVVDLAGNVGQTATQNVVVDTTSPEAAKSI
                     TITGISDDTGTSSSDFITSDTTLTVRGVLGAALGANEFAQISTDNGATWVNVTLAADG
                     LNWSYVDGRTLTNGTTTWQVRVVDLAGNVGATGSQSAQIDTVNPAQVLTIASISTDTG
                     SSATDFITSDTSLTLTGSLGAGLASGEVAQISLDGGATWTTLTTNGTQWTYTDSRTLT
                     DGSYVYQVRVLDLAGNTGPVVSKTVVVDTINPTATPGIVSYTDDVGQRQGTLSSSQAT
                     DDTTPLLNGVLSAPLASGEVVYLYRNGLLLGAVTMVGALNWTYSDSGLVSGAYTYSAR
                     VVDLAGNITSSSDFVLTVDTSIPTTLAQITSQTTRDTTPIISGVITAALASGQYVEVV
                     INGKTYTSEPGGAVVVDPAHNTWYVQLPDTDALTVSATAYTVTAQVKSSAGNGNNANI
                     SNGTVTVNAAIDYTPTWTTASKTTAWGLTYGLDSHGMWTVLANQQVMQSTDPLTWSKT
                     ALTLYQSGNNYATSSIADYDRNGTGDLFITRDDYGTGYINGFTNNGDGTFSSAIQVTV
                     GTLTWYGSIVAFDKEGDGYLDFWIGDAGGPDSNTFLWNNAGTLVGNSTTSNSGGSATV
                     GGAVTGYLSLNEGSGVDLNNDGRIDLVQHTYNLNNYYTLSSLINQGNGTFVWGQNTTN
                     TFLSGAGSGAMSSSVSMTWADFDGDGDMDLFLPASQGRANYGSLLFNTNGVLGSPVAV
                     GATATTYASQFSLAVDWNHDGLMDIARIAQTGQSYLYTNVGGASNWTQSALGGSQSGT
                     TSGVAAMDYDWDGAVDVLVTKQSGSVFLIRNTNTVSYGTSLHLRITDPNGINVYYGNT
                     VKLYNSAGVLVATQIINPQSGMGVNDTSALVNFYGLNAGETYNAVLIKSTGTTASNID
                     QTVNTSWGGLQATDATHAYDLSAEAGTASNNGKFVGTGYNDTFFATAGTDTYDGSGGW
                     VYSSGTGTWLANGGMDVVDFRLSTVGVTANLSSTAAQATGFNTSTFTNIEGISGSNFN
                     DTLTGSSGDNQLEGRGGNDTLNIGNGGHDTLLYKLLNASDATGGNGSDVVNGFTVGTW
                     EGTADTDRIDIRELLQGSGYTGNGKASYVNGVATLDAQAGNIGDFVKVTQSGSDTIVQ
                     IDRDGTGGTFATTNVVTLTGVHTDLATLLANHQLMVV"
     gene            22320..24482
                     /locus_tag="ACHKA1_002476"
     CDS             22320..24482
                     /locus_tag="ACHKA1_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFO5680115.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADVEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWSLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIVATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            24479..25654
                     /locus_tag="ACHKA1_002477"
     CDS             24479..25654
                     /locus_tag="ACHKA1_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO5680116.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            25651..26058
                     /gene="cueR"
                     /locus_tag="ACHKA1_002478"
     CDS             25651..26058
                     /gene="cueR"
                     /locus_tag="ACHKA1_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO5680117.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(26062..26265)
                     /locus_tag="ACHKA1_002479"
     CDS             complement(26062..26265)
                     /locus_tag="ACHKA1_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680118.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGIN"
     gene            complement(26262..26684)
                     /locus_tag="ACHKA1_002480"
     CDS             complement(26262..26684)
                     /locus_tag="ACHKA1_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680119.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPASRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(26785..28524)
                     /locus_tag="ACHKA1_002481"
     CDS             complement(26785..28524)
                     /locus_tag="ACHKA1_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO5680120.1"
                     /translation="MKSVKLSLLAVVVATIVSPSAFADDTVEAAATELTAIHPGMSHD
                     EIDQKINRFMIRTGNTEAAQDYLFSHGYMDPQTGDNIYPASTAVQTDSNITDQKQADR
                     DAAQDTAIQNAHNIASIAAANADDAKKALTTAQSAIDANKGAIADTQKDVADVKASAA
                     HANTNANTALMNGLKLSGAVSDNKNQIEKNSSSIAEQQEQMALSEQNQIHRDAAQDSS
                     IQDAQHSANWASLKADDAHHAIMVAQTDIDANKSAISDTQKDVAAVRTDVSAMKDDVA
                     DVKASADHANANANTALMNGQKLSGVVSDNKNQIEKNSTAITEQQEQITATQKTVAAT
                     GDVKTAARYQSMINALQTATADAQQSQSEAQQTQITEQQKQIAATEKTVAAIGDPQST
                     AHYQKMINAKLTAQNEANERSTADQEQRINELASNVATQQQIDSVQYGEQIRNLAQDS
                     AQAHEQIESLTQDSAQTHQQLTNTQKRVADNSQQINSLNNNFSSLKHEVEDNRKEANA
                     GIASAVAIASQPQVKTGDFMMVSAGAGTFNNESAVSVGASFNAGIHTVIKAGVSADTQ
                     SDFGAGVGVGYSF"
     gene            28890..29252
                     /locus_tag="ACHKA1_002482"
     CDS             28890..29252
                     /locus_tag="ACHKA1_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680121.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKGMGKTTEE"
     gene            complement(29533..29991)
                     /gene="ybbJ"
                     /locus_tag="ACHKA1_002483"
     CDS             complement(29533..29991)
                     /gene="ybbJ"
                     /locus_tag="ACHKA1_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO5680122.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(29988..30905)
                     /gene="qmcA"
                     /locus_tag="ACHKA1_002484"
     CDS             complement(29988..30905)
                     /gene="qmcA"
                     /locus_tag="ACHKA1_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO5680123.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            31051..31728
                     /gene="fetA"
                     /locus_tag="ACHKA1_002485"
     CDS             31051..31728
                     /gene="fetA"
                     /locus_tag="ACHKA1_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO5680124.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            31715..32494
                     /gene="fetB"
                     /locus_tag="ACHKA1_002486"
     CDS             31715..32494
                     /gene="fetB"
                     /locus_tag="ACHKA1_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO5680125.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(32557..33411)
                     /gene="cnoX"
                     /locus_tag="ACHKA1_002487"
     CDS             complement(32557..33411)
                     /gene="cnoX"
                     /locus_tag="ACHKA1_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO5680126.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(33472..34281)
                     /gene="ybbO"
                     /locus_tag="ACHKA1_002488"
     CDS             complement(33472..34281)
                     /gene="ybbO"
                     /locus_tag="ACHKA1_002488"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO5680127.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(34271..34894)
                     /gene="tesA"
                     /locus_tag="ACHKA1_002489"
     CDS             complement(34271..34894)
                     /gene="tesA"
                     /locus_tag="ACHKA1_002489"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO5680128.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            34865..35551
                     /gene="ybbA"
                     /locus_tag="ACHKA1_002490"
     CDS             34865..35551
                     /gene="ybbA"
                     /locus_tag="ACHKA1_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO5680129.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            35548..37962
                     /gene="ybbP"
                     /locus_tag="ACHKA1_002491"
     CDS             35548..37962
                     /gene="ybbP"
                     /locus_tag="ACHKA1_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO5680130.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMINLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LSEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMHQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         8689 a        10721 c        10951 g         7671 t
ORIGIN      
        1 aagttattac ccttcttaat aaaatgagac tggttataaa aaccagttaa aataatgacc
       61 tggaaggaag tgacttatct atggcgcgat tccagtttaa aagccgtaaa aataatgggt
      121 ttattttttt aataagtttt atggtgatgg gagaagctac aattgctgca ccgctgccac
      181 aatgggcgaa cgccccagcg gtaacgccag ttgcccaatt atccttacag gaaagtatat
      241 tacgcgcctt tgcgcgaaat cccgacgtca ctcaacaggc ggcgcagata ggcattggcg
      301 aagcgcaaat tgatgaagcc aaaagtgcct ggtatccgca cgttggatta accggcaacg
      361 cggggccgtc ccgacaaacg gactccagcg gcaggcttga taacaacgtt tcgtatggca
      421 taaccctgac acagctggtg tatgactttg gtaaaactaa caacgatatc aatctgcaaa
      481 ctgccgcccg tgacagctac cgctttaaat tgatggcaac cttaaccgat gttgcagaga
      541 aaacggcgac tgcctatatg gaagtcagtc gttatcaggc tttgtgcgat gcggctcaac
      601 gcaatattca ctcgctggaa aacgtctaca acatggcggc attgcgcgct aacgctggcc
      661 tgaactcatc gtcggatgaa ttacaggccc agacgcgtat tgccggaatg cgctcaacgc
      721 tggagcaata tcaggcgcag atggtgagcg ccaaagcgca actggcggtg ctcactggcg
      781 tacagccgga ggcgatagcc gcgccaccgg ctgaattagc cgagcagccg gtatcgctaa
      841 aaaatattga ttaccagtcc atcccgctgg tgctggcggc agaaaactta cgccagtcag
      901 cacagtacgg cgtggaaaaa acgaaagcgc aatactggcc aacgctcagt attcaggggg
      961 gtaaaacgcg ctaccagacc agcgaccgct cgtattggga tgatcagcta caactgaacg
     1021 ttaacgcgcc gctgtatcag ggcggcgcgg tttctgccca ggtgcaacag gccgaagggc
     1081 aacaaaaaat ctctgcctcg caggtcgaac aggcaaaact ggatgtgctg caacgagcgt
     1141 ctgtggcata tgcgaactgg accggcgcac ggggtcgtga agaagccggt ttagcgcaat
     1201 ccgaaagtgc gcacaaaacg cgagatgtgt accaaaatga atataaattg ggtaaacgca
     1261 gtctgaatga tctattaacc gtcgagcaag atgtctttca ggcgcaatcg gctgaaataa
     1321 atgccaatta tgatggctgg gtcgccgccg taaattatgc cgctgcggtg aataacctta
     1381 ttccattagc gggaattaaa cagggcttat acaacgactt acccgacttg aaataatcct
     1441 gccattatta ctagtgggcc gttgttaagt gattaccttt gtcttattta gataaatgga
     1501 ctcgctaagg atgtgcgcca tttatcagga atgccattat gagcctgatt attgatgtta
     1561 tttcgcgtaa aacatccgtc aaacaaacgc tgattaatcc tggcgacgtc acggttgtta
     1621 tttatgagcc ttccgtggtg caggttcatg ctcaggcctc tgccgttgcg cgttacgtcc
     1681 gtgaaggaaa tgacctgctg atctatatgc aggacggcac ggtgatccgc tgcaacggtt
     1741 atttcctgca agcggcgaat acagctgaac aatcggaact ggtgtttgcc gatggtcaac
     1801 agctaaccca tatcaccttt gccgatactg ctgcgggtgg attagccccc gtagaactga
     1861 ctgcccagac cactgcgatt gaaagcattg cgccatttct tgataccgtt gctcagacca
     1921 gcgctttccc gtggggttgg ctggcggggg cggcggtagg tggtggcgcg cttggtgcac
     1981 tgctggcaag cggtggcgat ggcgactcga aaacagaagt gattaataac cctacgccac
     2041 ccgctgagcc tggcaacgcc acaccatcat ttttagttac cgataatcag ggcgatcagc
     2101 gcggcattct cgccaccaat gacatcaccg atgacaccac gccaaccttt agcggcagcg
     2161 ggcaggcggg ggcgactatt cagattaaag acagtaacgg caatactatt gccagtactc
     2221 aggtagacaa caacggtcac tggagtgtct cgttacccac gcaaagtgca ggtgaacata
     2281 cctggtcagt ggtgcaaatt gtcggcagta ccatcactga cgccggttcg ataacgttaa
     2341 ccatcgacaa tagtcaggcc agcgtgcagg ttgccaccac cgcaggcgat aacattatta
     2401 acgccagcga acaggccgcc gggtttacgc tttctggcac cagtagccat ctggcgcagg
     2461 gaacagaact caccgtcaca cttaacggca aaacctacac cacaactgta ggggctaacg
     2521 gtgcctggag cgtgcaggtg ccgaccgccg atgcacaggc gttaggcgaa gggaatcagg
     2581 cggtactggt cagtgggaaa gacaccacag gcaatacggt caccggcgcg cagctgctaa
     2641 cggtcgatac ccaaccgcca acgcttgcca tcaacaccat cgcccaggac aacatcatca
     2701 gtgctgcgga acataacgtc gcgctggtac tgagcggcac gtcgaatgca gaagcggggc
     2761 aaaccgtaac actgaccgtc aacgggaaaa gccatacagc aaccgtcggt agcgacggaa
     2821 cctggcaagt gacgctgcct gccacggaag tccaggcact ggcggagggt aattacgctg
     2881 tcaatgccag tgtcagcgat cgggcaggga acaccaccag ccacagcgcg aatttcacgg
     2941 tagacacctc agcacccgtg gtcagtgtta ataccgtggc gggcgacgat attcttaata
     3001 atgccgagca ggccgtcgcg cagatcatct ccggacaagt tagcggtgct tctcctggcg
     3061 atacggtaac ggtgaaattg ggcactcatg tcctgacggg catcgtgctg gcagatggca
     3121 gctggaatgt ggcgttggac ccggcggtaa cccgcacgct ggatcgcgga gccaatacga
     3181 ttttcgtcac cgtgacagat gctgcaggaa atactggcgc ggcgtctcga gcaatcacgc
     3241 tggtcggtgt ttctccgttg atcaccatta acaccgtctc cggcgatgac attatcagtg
     3301 gcgcagaaaa aggtgcgcca ctgaccctta ccggtagcac tcaacaggct gagacaggac
     3361 aaaccgtcac agtaaccctg gctggacaga gttttaccac taccgtgcag gccgatggct
     3421 cctggagtct gacggtacct gccgccgcga tgggaaatct gcctgacggc gaggtggcga
     3481 ttaccgcttc tgtgacggat ctcagcggca ataccggcaa cacctcccgc accattaccg
     3541 tcgacagcca ggccccggcc ttaagcattg atccactgac cgctgataac atcattaacg
     3601 ccgccgaaag cgggcaggat ctgcccatca ccggcaccac cgacgcccag ccagggcaga
     3661 cggtaacggt gacgcttaat gggcagacgt atcagggcgt cgtgcagcca gacggcacct
     3721 ggagcgtgac cgttcccgcc gccaacgttg acgcgctggc tgacggtaac gccacggtca
     3781 ccgccagcgt gaacgatgtc gccggtaacc cgaccagcgt ctcacgcgtg gcgctggtcg
     3841 atgccacgcc gccggtggtg accattaatc cggtggcgac cgataatgtc attaacacgc
     3901 cggaacatat ccaggcgcaa attatcagcg gcacggtcac tggcgctcag gcgggcgata
     3961 tcgtcaccgt gacgctgaat aatgtggatt acaccacggt ggtggatggt tccggcaact
     4021 ggagtctggg cgttccggcc tcggtggtca gtgggctggc ggacggcagt tatcctgtca
     4081 gcgtctcggt aaccgacaaa gccggaaaca cgggcagcca gtcattgacc gtcacggtca
     4141 ataccgccgc gccccttatc ggcattaaca gcattgcggg cgatgatgtg attaacgcca
     4201 gcgaaaaagg ggccgatctc cagattaccg gcaccagcga tcagcctgtt aacaccgcca
     4261 tcaccgtgac gctgaacggg caaaattaca ccaccacgac cgacgcctcc ggcaactgga
     4321 gcgtcaccgt tccggcatcg gcggtaaccg cactgggcca ggccaactac acggtaacgg
     4381 cggcggtgac cagcagtatc ggcaatagca ccaccgccag ccataacgtg ctggtcgaca
     4441 gcgcgctgcc cggcgtgacc attaacccgg tggcaaccga cgatattatt aacgccgccg
     4501 aagcgggcgt ggcgcaaacc atcagcgggc aggtgacagg agccgccgtt ggcgacgcgg
     4561 taaccgttac gctgggtggc aataactaca ccaccaccgt gcaggccggc ttaagctgga
     4621 gcgtggacgt tccggcggac gctattcagg cgctgggtaa tggcgattta acggttaatg
     4681 cctcggtcac caatcaaaac ggcaacaccg gcagcggcac gcgggatatc accatcgacg
     4741 ccaatctgcc cggcctgcgg gtcgatacgg tggcgggcga tgatgtggtc aatatcatcg
     4801 agcacgggca ggcgctggtg gtcaccggca gcagcttggg gcttgctgaa ggcacgccgc
     4861 ttaccgtcac gattaacgat gtggaataca ccactgcggt gcaggccgat ggcagctgga
     4921 gcgtgggcat cacggcggcg caggttagcg cctggcctgc gggggcggtt aatattgccg
     4981 tatccgggga aagtagcgcc ggaaatccga taagcattac gcatccggta acggtggatc
     5041 tcactccggc ggcgatcacc atcaacacca tcgccacgga tgacgtgatt aacgctgcag
     5101 aaaaaggggc taatttaact ctttccggca ccaccactaa cgtagaagca gggcaaaccg
     5161 tcactgtcac ctttggcggt aaaaattaca ccgccagcgt ggcgggcgat ggtagctgga
     5221 ctgccaccgt tcccgccgcc gatctggcag cattaccaga gggcagcgcc tccgcgcagg
     5281 ccagcgtcag caatatcaac ggcaatagcg cctcggcggt gcacagctac agcgtcgaca
     5341 gcagcgcgcc aaccatcatt atcaataccg tcgccagcga caatatcgtc aacgccagcg
     5401 aagccgatgc gggtgtgacg gtgagcggca gcaccaccgc tgaagcgggg cagattgtta
     5461 cggtaacgct caatagcccg accgtgcaga cgtatcaggc aacggtgcag gcggacggca
     5521 gctggagcat caatattccg gcggcagatc ttgaggcatt gaccgatggc agccacaccc
     5581 tgaccgccac ggtcaatgac aaagcgggca acccggcgag caccacgcat aatctggcgg
     5641 tggatctcac cgttccggta ctcaccatca acaccattgc gggcgatgac attataaacg
     5701 ccaccgaaca cgggcaggcg ctggtgattt ccggttccag cactggcgga gaagcgggag
     5761 atgtcgtcac cgtcacgcta aacagtaaaa cctacaccac gacgctggat gcctccggca
     5821 actggagcgt cggcgttccg gcggcggatg tcacggcgct gggtagcggc ccgcaaaccg
     5881 tgactgcgac cgttaccgat gccgccggta acagcgatag tgagacgcac acggtcaccg
     5941 ttaaccttac cgcgccgacc attggcatta acaccatcgc caccgatgac gtgattaacg
     6001 ccaccgaaaa aggcgcggat ctgcaaattt ccggcaccag taatcagcca gcaggcacca
     6061 caattacgct gacgctgaac gggcaaaatt acactgccac cacagatgct tccggcaact
     6121 ggagcaccac agtgccagct tccgcagtcg gtgcgctggg cgaagccagc tacacggtga
     6181 cggcgaacgt caccgacagc gcgggcaaca gcaactccgc cagccataac gtgcaggtga
     6241 ataccgcgct gcctggcgtc acccttaatc cggtagcgag cgacgatatt atcaacgctg
     6301 ccgaatcggg cgtggcgcag accatcagcg gccaggtcac gggcgcggcg gcgggcgata
     6361 cggttaccgt aacgcttggc gggaaaacct acaccgctac cgtgcagggg aatttaagct
     6421 ggagcgtgga cgttccggcg gcggatattc aggccatcgg caatggcgag ctgacggtta
     6481 acgcctgggt aaccaacggc gttggcaata ccggcagcgg ttcgcgcgat attactatcg
     6541 acgccaacct gcctggcctg cgcgtcgata ccgtggcggg cgatgatgtg gttaacagca
     6601 tcgaacacgg tcaggcactg gtgataactg gtagcagcag cgggctggcg gcgggcgctg
     6661 cgctgacggt ggtgatcaat aacgtcacct atggcgcgac agtattagcc gatggcacat
     6721 ggagccttgg cgttccggcg gcaaatgtag gcaactggcc tgcgggtacg gtgaatatca
     6781 cggtttcagg tgctactagc gcaggaaacc cggtcaccat cacccatcca gtcaccgtcg
     6841 atctggcagc tgtggcgatc tccattaaca ccgtttccgg cgacgatgtg attaacgccg
     6901 ccgaaaaagg ggcagattta accctttccg gcagcacctc cggcgtggag ccagggcaaa
     6961 cggtcaccgt cacctttgac ggcaaaacct acaccgccac cgtagcaggc gatggtagct
     7021 ggacaaccac cgtccctgcc gccgatctcg ccgcattacg cgacggcgac gccacggtac
     7081 aggccagcgt cagcactatt aacggcaaca cggcttcggc aacccacgtc tacagcgtcg
     7141 atgccacgac cccgacgctt accattaaca ccatcgccac cgacgatatt ctgaacgccg
     7201 ccgaggcggg taatccgcta accatcagcg gcagcagtac cgccgaagcg gggcagacgg
     7261 taaccgtcac gcttaatggc gtgacttaca ccggcaccgt tcaggcggac ggtagctgga
     7321 gcgtcagcgt gccgacggcg gatctcagca atctgaccgc cagccagtac accgtcagcg
     7381 cctcggtaaa cgataaagcg ggtaacccgg tgtccgccac tcacgggctg gcggtggatc
     7441 tcaccgtgcc ggtgctgacc attaacaccg tctcgggcga tgacattatt aacgctactg
     7501 aacacgggca ggcgctggtg atctccggtt ccagtactgg cggcgaagcg ggcgatgtca
     7561 tcaccgtcac gttaaacagt aaaacctaca ccaccacgct ggacgcttcc ggcaactgga
     7621 gcgtcggcgt tccgctgtct gatgtcaccg cgcttggcag cggcccgcaa aacatcacgg
     7681 ccacaattac cgacgcggca ggcaacagcg atgacgccag ccgcacggtc accgtgaatc
     7741 tcgccgcgcc aaccattggc atcaacacca tcgccaccga tgacgtgatt aacgccacgg
     7801 aaaaaggcgc ggatctgcaa attaccggta ccagtaatca gccagcgggc accacgataa
     7861 cggtgacgct gaacgggcaa aattacaccg ccaccacaga tgcttccggc aactggagcg
     7921 ccacggtgcc agcctcggca gttagcgcat tgggtgaagc taactacacg gtaacggcaa
     7981 acgtcaccga caaggcaggc aacagtaatt ccgccagtca taatgtgctg gtcaacagcg
     8041 ccttgcctgc cgttaccatt aacgcggtgg cgactgacga tattatcaat gccgacgagt
     8101 cggacaatgc gcaaaccatc agcggccagg tgacgggggc cgcggcggga gataccgtta
     8161 ccgtgacgct gggcggcaac acctacaccg ccacggtgca ggctaattta agctggagta
     8221 tcagcgttcc ggcggcggat attcaggcgc tgggtaacgg cgacctgaca attaatgcct
     8281 tggtcaccaa tggcgtgggc aacaccggca gcggctcgcg cgatatcacg atcgacgcca
     8341 atctgcctgg cctgcgggtc gataccgtgg cgggcgatga tgtgattaac agcattgagc
     8401 acaatcaggc gctggtgatc accggcagta gcaccgggct gacggcggga acggcgttaa
     8461 cggtcgagat taacaacgtt acttacgctg ccacggtgtt agccgacggc acgtggagcc
     8521 tcggtgttcc ggcggtagac gtcagcaact ggccagcggg tacggtgaat atcacggtaa
     8581 gcggcaccaa cagcgccgga acaacctcca ccattaccca tccggtcacc gtcgatctgg
     8641 cggcggttgc catcaccatt aacacccttt ccggcgatga tgtgatcaac gccgtcgaaa
     8701 aaggcgaaac gctggtggtg agcggcagca ccagcggtgt ggaagccggg cagacggtga
     8761 ccgtcacctt tggtggcaaa aattacacca ctaccgtgga agccaacggc agctggacgg
     8821 tgaatgtgcc gcctgccgat ctcgctgcgc tgccggatgg cgcgggcaac gtgcaggcga
     8881 gcgtcagcaa catcaacggc aataacgccc aggcggatcg cgcgtacagc gttgatgcca
     8941 ccgcgccact tgtgaccatc aacaccatcg ccagcgacga tactcttaac gtgagcgaag
     9001 cgggcgcggg gataaccatc agcggtacca ccacggcgca ggccgggcag acgctcaccg
     9061 tcacgctcaa taacaacacg taccagacca ccgttcaggc ggatggcacc tggagcgtga
     9121 atgttccggc agcggattta agcggattaa ccgccagcag ttacaccgtg accgccacgg
     9181 tgagcgacaa agcgggtaac ccggcaagcg ccgaccacgc gctggcggtg gacgttaccg
     9241 caccggatct caccattaat accgtcgcgg gcgatgacat tatcaacgcc atcgaacacg
     9301 gtcaggcact ggtcgtcagc ggaaccagta cgggcgcggc ggcgggcgat gtggtcacgg
     9361 ttaacctgaa cggtaaaaac tacaccacca cgctggacgc ctccggtaac tggagcgtgg
     9421 gcattccggc ggcggatgtc acggcgctgg caaccggcag ccagacgatc accgccagcc
     9481 tgagcgatcg cgcgggcaac agcgacagca ctacccataa tgtgaccgtt gatctcagcg
     9541 gcccgacgct gaccatcagt atcgtctccg gtgatgacat tattaataat gcggagaaaa
     9601 cccaggatct aatcatcagc ggcggctcca gcggcctggc gaccggcact acggtcaccg
     9661 tgatgttgaa cggcctggcg tacagcgcaa ctaccgacgg ttctggcaac tggagcgtta
     9721 ctgtgcctgc ttcggcggtc ggcgcactgg gcgaggcggt gtaccagatt agcgccagcg
     9781 ctaccgacag cgcgggcaac agcggcatca ccacccatac ggtgaatgtt gaatcgctcc
     9841 tgccaggggt gatcattaat accgtggcgg gcgatgacat tatcaacgcc gctgaaattg
     9901 gtgttaacca gaccatcagc ggacaggtta cggggacggc agcagccgga gatacggtca
     9961 ctgtcacgct cggcggtaat caatacgtcg ccaccgtaca gccagattta agctggagcg
    10021 tcagcgtacc ggcgaacgtt ttgcaggcgc tgggcaacgg tgagttgacc atcagcgcct
    10081 cggtgaccaa ttccgcaaat aatactggca ccacaacgca cgacatcgtg atagatgcca
    10141 acctgccagg cctgcgggtc gataccgtgg caggcgatga tgtgattaac agcatcgagc
    10201 acactcaggc gttggtgatc accggtagca gtagcggact ggcggcgggc gcggcgttga
    10261 cggtggttat caatagcgtc acctacggcg caacggtttt agcggatggt agctggagcg
    10321 ttggtgttcc ggcggcagat gtcacaaact ggcctgcggg gacggtcaat attgccgtct
    10381 ctggcaccaa caccgccgga acgacaacca gtattaccca tccggtgacc gtcgatctcg
    10441 cagccgtggc gatcaccatc aacccccttt ccactgacga tgtgattaac gccgctgaga
    10501 aaggctctga tttgcagctc tccggcacca cctccgatgt ggaagcgggg caaaccatca
    10561 ccgttatctt cggcggcaaa agctacacca ccacggttgc cgcggataat acctgggggc
    10621 tgacaatccc agccgccgat ctcgccgctt taccggatgg cgcagcaaac gtccaggcca
    10681 gtgtcagcag cgtcgcaggt aacagcgccc aggcaacgca tgtttacagc gttgatgcca
    10741 ccgcgccctc cgtcaccatc aacaccatcg ccgcagacga tattcttaac gccgctgaag
    10801 cgggatcggc gctgatcatc agcggcacca gcacgacaga agccgggcag acggtaaccg
    10861 tgacgcttaa cggcgttaat tacagcggca acgtgcaggc tgacgggagc tggagcgtca
    10921 gcgtaccaac tggtgattta gctaacctta ccgccagctc gtataccgtt aacgcctcgg
    10981 tcagcgacaa agccggaaat ccggcttcgg caacgcataa tctgacggtg gaccttgccg
    11041 ctccggtcgt caccatcaac acggtggcgg gcgatgacat catcaacgcc acggaacacg
    11101 gacaggcgca gatcatcagc ggctcggcaa cgggcgcgac taccggtaat acggtttccg
    11161 tgacgattgg cacgaccacc tataccaccg tgctggacgc caacggcaac tggagcatcg
    11221 gcgtgcctgc cagcgtgatt tccgcactgg cgcagggcga tgtgaacatt agcgccacgg
    11281 ttaccgactc cgcaggcaac agcggcacag cctcgcacgt tgtcagcgtg gcgctcggcg
    11341 ctccggtgct cgccattaac accattgccg tcgatgacat catcaacgcc acggagaaag
    11401 gcgcggatct ggcgatttcc ggcaccagcg accagcctgc gggcacgcag attaccgtta
    11461 cgctcaacgg gcaaaattac accaccacag ccgatgcttc cgggaactgg agcgtgaccg
    11521 ttccggcgtc tgcggttggt acgcttggtg aagcgactta taccgtgacc gcctccgcca
    11581 ctgacgccga tggcaacagc ggttccgcca gccataacgt acaggttaat accgcgctgc
    11641 cgggcgtcac cattaacgtg gtggcaacgg acgatattat taacgccgcc gaagcgggcg
    11701 tggaccagac catcagcggg caggtgacgg gtgcggcggc aggcgacacg gtgaccgtca
    11761 cgctcggcgg ggcgacttac actgccacgg tgcaggcgaa tttaagctgg agcgtcgatg
    11821 ttccggcctc cgcgttacag gcgttgggca acggtgagct gaccatttcg gcttcggtaa
    11881 cgaacagcgt aggcaatacc ggcaacggca cgcgtgaaat caccatcgac gcgaatctgc
    11941 caggtctgcg ggtcgatacc gtggcgggcg atgatgtggt caatatcatc gagcacgggc
    12001 aggcgctggt gattaccggc agcagctccg atctggcagc gggcagcaac gtcacgctga
    12061 ccataaacgg gcaaacgtat gttgcggcgg tgctggcgga tggctcatgg agcgtcggtg
    12121 ttccggcggc agatgtcagc gcctggcctg cggggacggt cacgattaca gccagcggca
    12181 acaccaccgc cggaaacccg gtgagcgtta cgcatccggt gactgtcgat ctcacggcgg
    12241 tggcggtcag catcaacgcc attaccgtcg atgatgtgat taacgccgct gaaaaaggcg
    12301 cggcgttaac gctctccggc agcacctctg gcgtcgaagc cgggcaaacg gtcaccgtga
    12361 actttggcgg aaaaacctac actgcaagcg tggcgacgaa cggttcctgg agcaccacgg
    12421 ttccggcggc agatatggcg gccctgcgtg atggcgatgc cagcgcacag gccagcgtca
    12481 gcaatgttaa cggcaacagc gccaccacta ctcacgctta cagcgtcgat gccagcgcgc
    12541 caacggtgac cattaacacc attgcgggcg acgacattct taacgccgcc gaagccggag
    12601 cggcgctgac catcactggc agcagcacgg cggaagcggg gcagacggtg accgtcaccc
    12661 tcaatggcga aaactatact ggcacggttc aaacggacgg tagctggagc gtcagtgtgc
    12721 cgcaagccga cgtaagcgcc ctgaccgcca gcaactacac cgtgagcgcg gcggtgaacg
    12781 acaaagccgg aaacccggcc tcggttaacc acaacctgac ggtggatact tctgttccgg
    12841 tcgtcaccat caacacggtg gcaggcgatg atgtgatcaa cgcgacggaa cacgcccagg
    12901 cacagatcat cagcggatcc gccacgggtg cggcaaccgg tagcaccgtg acggtgacta
    12961 tcggcacaaa tactttcacc acggtgctgg atgccagcgg caactggagc gtcggcgttc
    13021 cggcaagcgt cgtctcggca ctggcgaacg gcacggtgac cattaacgcc agcgtcaccg
    13081 atgtcgccgg taacagcggc agcgccacgc atcaggtgac ggtcaatacc gggctgccga
    13141 ccattacttt taacgccatc agcggcgata acgtcctgaa cgctgatgaa aaaggccagc
    13201 cgttgaccat cagcggcggc agtacggggc tggcgacggg cgcgcaggtc accgtcacgc
    13261 tcaacggtca caactacagc gccaccaccg acgcgtcggg caactggacc ttaaccgtgc
    13321 cggtgagcga tctggcggca ttaggtcagg ccaactatac ggtcagcgcc agcgccacca
    13381 gtgcagcagg caacaccgcc agcagccagg cgaatttact ggtcgacagc ggcctgccgg
    13441 acgtcaccat caacaccgtg gcaggcgacg atattatcaa cgccgccgaa gcgggggccg
    13501 atcaaaccat cagcggggtg gtgactcgcg ccgccgctgg cgatacggtc accgtgacgc
    13561 tgggcgggaa cacttacacc gctacggtac agagcaactt aagctggagc gtcagcgttc
    13621 cgacagccga tctccaggcg ttgggcaatg gtgatttgac cattaccgcc tcggtcacca
    13681 acgctaatgg caacaccggg agcggcacgc gggatatcac cattgatgcc aacctgccgg
    13741 ggctgcgcgt agataccgtg gcgggcgatg atatcgtcaa cagcatcgag cacgggcagg
    13801 cgctggtgat caccggcggc agtagcggcc tgaatgcagg tgctgtgctg acggttacca
    13861 tcaacagtgt ggcgtattcc gccacggtgc aggcggacgg aagctggagc gttggcattc
    13921 cggcggcaaa cgtcagcgcc tggcctgcgg ggccgttaac cgttgaggta gccgggcaaa
    13981 gcagcgccga taacccagtc agcgtcagcc atccgttcac cgtcgattta acggcggtgg
    14041 caatcagcat caatacggtc gccagcgacg acgtgattaa cgccgccgag aaaggcacca
    14101 atctgaccct ttccggcagc accagcggga ttgaaagcgg gcaaaccgta accgtcacct
    14161 ttggcggcaa aacctacacc gcaagcgtcg ccgccaacgg aagctggagc aaaacggttc
    14221 cggtggcaga tctggcaact ctgccagacg gcgcggcgaa cgtgcaggcc agcgttagca
    14281 gcgcgagcgg taacagtgcc tcggcgaccc atgcgtatag cgttgacgcc agcgcgccga
    14341 cgctcaccat taacaccatc gccagcgacg atatccttaa cgccacggag gccggaagcc
    14401 cgctcaccat cagcggctcc agcaccgccg aaaccgggca gacggtcacc gtcaccctta
    14461 acggcgcaac ctacaccggc actgtacagg cggacggcgg ctggagcgtc agcgttccta
    14521 cttcagccct gggcgcgctc aacgcaagca attacaccgt cagcgccacg gtcaacgaca
    14581 aagcgggcaa ccccggcagc gccagccaca atctggcggt agataccacc gcgccggttc
    14641 tcaccattaa caccgtggcg ggcgatgaca tcatcaacga cgccgaacac gcccaggcgc
    14701 tggtgatctc cggtaccagc acgggcgggg aagcgggcga tgtggtgagc gttgtgctca
    14761 atggcaaaac ctacaccacc acgctggatg cctccggtaa ctggagcgtc ggcgtaccgg
    14821 cggcggatgt tgcggcgctg ggtagcggcg cacagaccat caccgccagc gtcagcgatc
    14881 gggcaggcaa cagcgacgac gccagccgca ccgtgaccgt cagcctcagc gcgccagtga
    14941 ttagcatcaa caccatcgcg ggcgatgatg tgatcaacgc gacggaaaaa ggatctgatc
    15001 tggcgctttc tggcaccagc gatcagcctg cgggtacggc gatcaccgtc accctgaacg
    15061 gacaaaacta cagcgccacc acggatgcct ccggcaactg gagtgttacc gtgcctgcct
    15121 cggcggtcag tgcgctgggc gaagcgacct acagcgtgac ggcgagcgtc accaatgctc
    15181 agggtaacag cagcaccgcc agccataacg tgcaggttaa taccgcgctg ccgggcatca
    15241 ccattaatcc ggtggcgacg gacgatatta tcaacgcttc agaggcaggc agcgcgcaaa
    15301 ctatcagcgg ccaggtaacc ggtgcggcgg cgggcagcac cgtcaccgtt gaactgggcg
    15361 gtaaaactta caccgccacc gtgcagccag atttaagctg gaatgtaagc gtacctgccg
    15421 ccgactggca ggcgctgggt aacggtgagt taacggttaa tgcctcggtg accaacgccg
    15481 ttggcaatac cggcagcggc acgcgcgata tcaccatcga tgccagtctg cccggcctgc
    15541 gggtggatac cgtcgcggga gatgatgtgg tcaatatcat cgaacacagt caggcgcagg
    15601 tgatcaccgg cagcagctcc ggctttgccg caggcacggc gttgacggtg gtgattaaca
    15661 accaaactta cgccgccacg gtgctggtga atggtacctg gagcgtcggc gttccggcgg
    15721 cggatgtcag taactggcct gcgggaacgc tgaatattac cgttagcggc gcgaacagtg
    15781 ccggaacgca aaccagcatt acccatccgc tgactgttga tctcactacc gttgccatca
    15841 gcattaacgc tattacccct gatgatgtga ttaacgccgc cgaaaaaggc gcggcattaa
    15901 cgctctccgg cagcacgtcc ggcgtcgaag cggggcaagc cgtcaccgtc acctttggcg
    15961 gtaaaaccta caccaccacg gtggcggcaa acggtagctg gagcaccact gttccggctg
    16021 cagatttggc ggcgctgcgt gacggcgatg ccagcgccca ggtgcgggtg actaacgtca
    16081 acggcaacag cgccacagcc acgcatgaat acagcgtcga tagcgccgcg ccaactgtga
    16141 ccatcaacac cattgccagc gataacatca tcaacgccag cgaagcggcg gcgggcgtaa
    16201 cggtgtccgg caccagcacg gcgcaaaccg ggcagacgct caccgtcacg cttaacggca
    16261 ctaactacca gaccacggtg caggcagacg gcagttggag cttaacgctg cccgccagcg
    16321 acttaaccgc actggccaat aacggctaca ccctgaccgc tacggtcagc gatctggcgg
    16381 gtaaccctgg cagcgccagc aaaggtgtga ccgtcgatac caccgcgccg gtgatcagct
    16441 ttaacaccgt ggcgggcgat gatgtgatta acaacgtcga acacactcag gcgcagatta
    16501 tcagcggcac cgccacgggc gcggtcgcag gcgaccgcct ggtggtgacc atcgccgggc
    16561 agcagtatgt caccagcacc gatgccagcg gcaactggag cgtcggcgtg cctgccagcg
    16621 tgatttccgg ccttgcggac ggcacggtga ccatcagcgc caccattacc gatagcgcag
    16681 gcaacagcag cacgcagacg cacaacgtac aggtgaacac ggcggcagtg tcgctttcgg
    16741 tcagcaccat cagcggcgat aatattatta acgccgccga agcgggcagt gcgctgaccc
    16801 tgagcggcac tggcactaat ttcgcgacag gtacagtggt gaccgtgttg cttaacggca
    16861 aaggctacag cgccaccatt cagagcaacg ggagctggag cgtgaacgtg cctgcggcgg
    16921 atgttgcggc gctcagtgat ggcaccagct acacggttag cgcctccgct caggacagtg
    16981 ccggaaacag cgccacggct tcgcgcagcg tggcggtgga tctcaccgcg ccggtgatca
    17041 gcattaacac cgtttcgacg gatgaccgac tcaacgccgc cgaacaacag cagccgttaa
    17101 cccttaacgg ctcgaccagc gcggaagtgg gacagacggt caccgtcacc tttggcggca
    17161 aaacctatac cgccacggtt gctgccaatg gcacctgggc gctgaacgtg cctgcggcag
    17221 atctggccgc gctcgggcag ggcgcgcaga ccattaccgc cagcgtgaac gatcgcgccg
    17281 gtaaccccgg acaggccacg cacgccctga ccgtcgatac cgttgcgcca acggtcacca
    17341 tcgccacggt ggcgggtgac gatattatca acaacgccga gcagcttgcc gggcagacca
    17401 tcagcggcac caccaccgcc gaagtaggcc agacggtgac cgtcactttt aatggtcaga
    17461 gctggagcgc aacggtcggc agcggcggaa gctggtcggt atttattccg gcgcagcagt
    17521 ttgccggatt aagcgacggc agctacacca tcagcgcgac ggttagcgat caggccggaa
    17581 accccggcag cgccagccgt ggcgtgacgc tgaacggcga cgtgcccacc gtcaccatca
    17641 acacctttgc gggcgatgat gtggtgaatg cagcagaaca cggctcgtcg ctggtgatca
    17701 gcggcaccac caccgcgccg gtcggacaga cgctgaccct gaccttaaac ggcaaaacct
    17761 acaccaccac ggtacagacc ggcggtagct ggagctacac cctaggcagc gccgatgtta
    17821 ccgcgctggc ggacggcaac gcctacgtga ttaacgcctc ggtgagcaat gccattggca
    17881 acaccggtag cagtaatcac accattaccg tcgatctcag cgctccggcg atgggcatta
    17941 atatcgattc cctgcaagcc gacactggcc ttagcgccag cgactttatc accagcgtca
    18001 gtccggtagt ggtcaacggc tcgctcaccg ccgcgcttgc cagtaacgag acggcgcaaa
    18061 taagtatcga cggcggcaca acctggacca cgcttaccgt taccggcacg acctggcgct
    18121 ataacgacag ccgcacgctg accgatggca actatctcta tcaggtgcgg gtgattgacg
    18181 cagcgggcaa cgttggcgcg accgacagcc agaatgtggt gatcgacact accgcgccag
    18241 atcccgcggt gaaaaccatc gccatcagcg cgatcaccac cgatacgggc ttgatcacta
    18301 acgattttgt caccagcgac acgacgcttg cggtgagcgg cacgctgggg gcgacgcttt
    18361 ctgccggtga gttcgcgcaa atcagcattg atggcggcac cacctggcag aatctttcgg
    18421 taagcggttt gacctggagc tatcttgacg ggcgtacgct gaccgatggc aactacaact
    18481 accaggtgcg ggtgatcgac accgccggaa atatcggcgc gaccgccagc cagatagtca
    18541 ctgtggatac caccgcgccg ctggcgagca aaaccatcgc cattgcgagc atcagcgatg
    18601 acaccgggtt aagcagcagc gattttgtta cacgcgacac cacgctgacc gtgcgcggca
    18661 cgctcggcgc ggcgcttgcc gccgatgaac gggcgcaaat cagcctcgac ggcggcgtca
    18721 cctggactac gttaaccgtt atcggcacca gctggagcta tgccgatggt cgcacgctta
    18781 ctgacggcac ctggaactac acggtgcggg tggtggatct ggcggggaac gttgggcaga
    18841 ccgcaacgca aaacgtggtg gtcgatacca ccagcccgga agcggcgaaa agtatcacca
    18901 ttaccggcat cagcgatgac accggaacca gcagcagcga ttttattacc agcgacacca
    18961 cgcttaccgt gcgcggcgtg ttgggcgcgg cgctcggcgc taatgagttc gcacaaatca
    19021 gtaccgacaa cggcgcaacc tgggtgaacg tgacgctagc agcagacggc ctgaactgga
    19081 gttacgttga cggacgaacc ctcaccaacg gcaccaccac ctggcaggtg cgggtggtcg
    19141 atctggcggg caacgttggc gcaaccggca gccagtcggc gcagattgac accgttaacc
    19201 cggcgcaggt gttgaccatc gccagcatca gcaccgacac ggggagttcg gcaactgact
    19261 ttatcaccag cgatacctcg ctcacgctaa ccggttcgct gggagcgggg cttgccagcg
    19321 gcgaagtggc gcagattagc cttgatggcg gcgcgacctg gactacgctc accaccaacg
    19381 gcacgcagtg gacctacact gacagccgca cgctgaccga cggcagctat gtttatcagg
    19441 tgcgggtgct ggatctggcc gggaacaccg gtccggtggt gtcgaaaacg gtggtggtcg
    19501 atacgattaa ccccaccgcc acgccaggta ttgtgtcgta taccgatgat gtcgggcagc
    19561 ggcaggggac gttaagcagt tcgcaggcca ccgacgacac tacgccgctg ctgaacggtg
    19621 tactttccgc gccgcttgcc agcggtgaag tggtctatct ctaccgtaac ggcctgctgt
    19681 taggtgcggt aacgatggtc ggcgcactga actggaccta cagcgacagc gggctggtca
    19741 gcggtgccta tacctacagc gcgcgagtgg tggatttggc ggggaatatc acctcctcca
    19801 gtgattttgt cctgacggtc gatacctcta ttccgaccac gctggcgcaa atcaccagcc
    19861 agaccacgcg cgatactacg ccgattatca gcggggtgat caccgccgcg cttgccagtg
    19921 ggcagtatgt tgaagtggtg atcaacggca aaacctacac ctctgaaccg ggcggcgcgg
    19981 tagtggtcga tccggcgcac aacacctggt atgtacagtt gccggatacc gatgcgctga
    20041 cagtttccgc gaccgcctat accgttactg cacaggtaaa aagttcggcg ggtaacggca
    20101 ataacgccaa tattagcaac ggcacggtga cggttaacgc ggcgattgat tacacaccga
    20161 cctggaccac cgccagcaaa accaccgcct gggggctgac ctacggcctc gactcgcacg
    20221 ggatgtggac ggtgctggca aaccagcagg taatgcaatc gactgaccca ctcacctggt
    20281 cgaagaccgc gctgacgctg tatcagagcg gcaacaacta cgccaccagc tccatcgccg
    20341 attacgaccg taacggcacg ggcgatctgt ttatcacccg tgatgactac ggtacaggct
    20401 atatcaacgg ctttaccaat aacggcgacg gcaccttttc cagcgctatt caggtcaccg
    20461 tcggcaccct gacgtggtac ggctcgattg tggcatttga taaagagggc gacggctatc
    20521 tcgacttctg gattggcgac gcgggcgggc cggactccaa cacctttctg tggaacaacg
    20581 caggcacgct ggtaggcaac tccaccacgt cgaacagcgg cggtagcgcc acggtgggcg
    20641 gggcggtgac ggggtatctt tcgctcaacg aaggttctgg cgtcgatctg aacaatgacg
    20701 gcaggatcga cctggttcag cacacctata acctgaacaa ctattacacg ctgtcttcgc
    20761 tcatcaacca ggggaatggg acgtttgtct gggggcagaa caccaccaat accttcctga
    20821 gcggggcggg tagtggcgct atgagcagca gcgtttccat gacctgggcc gatttcgatg
    20881 gtgacggcga tatggatctc ttcctgcccg ccagccaggg aagagctaac tacggctcgc
    20941 tgttattcaa caccaacggc gtactgggta gcccggtggc ggtgggcgca acggcaacca
    21001 cctacgccag ccagtttagc ctggcggtgg actggaacca tgacggcctg atggatatcg
    21061 cccgtatcgc ccagaccgga caatcgtatc tttacaccaa cgttggtggt gccagcaact
    21121 ggacacaatc agccctcggc ggcagccaga gcggtaccac cagcggcgtg gcggcaatgg
    21181 actacgactg ggacggcgcg gtggatgtgc tggtgaccaa acagtcgggc agcgtgttcc
    21241 tgatccgcaa caccaacacg gtgagctacg gcacttcgct acacctgcgc atcaccgatc
    21301 ccaacggcat taacgtctat tacggcaata ccgtgaagct gtacaactcg gcgggagtgc
    21361 tggtcgccac gcaaatcatc aacccgcagt cgggtatggg ggttaacgac acctcggcgc
    21421 tggtcaactt ctacgggctg aatgccggag aaacctacaa cgcggtgctg atcaaatcca
    21481 ccggcaccac cgccagcaat atcgaccaga cggtcaacac cagctggggc ggtttacagg
    21541 ccaccgatgc cactcacgct tacgacctca gcgccgaagc gggtaccgcg agcaacaacg
    21601 gcaagttcgt cggcaccggt tataacgaca ccttctttgc caccgcaggc accgatacct
    21661 acgacggttc cggcggctgg gtgtacagct ccggcaccgg aacgtggctg gcgaacggcg
    21721 ggatggacgt ggttgatttc cggctttcga cggtgggcgt gacggctaac ttaagcagca
    21781 ccgccgcgca ggccaccggt tttaacacct cgacatttac caatatcgaa ggcatttccg
    21841 gatcgaattt taacgacact ctgaccggca gcagcggcga taaccaactg gaagggcgcg
    21901 gcggcaacga cacgctcaac atcggcaacg gcggccacga caccttgctc tataaactgc
    21961 tcaacgccag cgacgccact ggcggcaacg gttcggatgt ggtgaatggc tttacggtag
    22021 gcacctggga aggcactgcc gacaccgatc gcattgatat ccgcgagttg ttgcagggca
    22081 gcggctacac cggaaacggc aaagccagct atgtcaacgg cgtggcgacg ctggatgcgc
    22141 aggccggaaa catcggtgac tttgtcaaag tcacccagag cggcagcgac accatcgtgc
    22201 agatcgaccg cgacggcacg ggcggcactt ttgcgacaac taacgtggtc acgctgacgg
    22261 gcgtgcacac cgacctcgcc actttgctgg cgaatcatca gttgatggtg gtgtagataa
    22321 tgaaaaaaaa tgcccagtcc gtggaagcct ggctggaggc gatgatcgcc gtggcgcgct
    22381 attatcggct cgatttttct caggagaatg tgcgcgcgac cgtcaactgg gagcgcgaca
    22441 gtaagcggga agagttgctc accgacatgg cgcggcagtt gggaatgggg ttacggctgg
    22501 tggagttttc cgccgattcg cttaacccct ggcgtctgcc gctgatcgcg gtgtttgata
    22561 atcagcagat tggcgtgatc acccgtcgcg acaatcatga caatatcagc gttcagttca
    22621 gcggtgacga agggctggaa accacgctta acgtcgcgga tgtcgaggat aaaatcgttg
    22681 aactggcgct actgcgcccg ctcagcgcca tccctgacgc gcgggtagat gactatattc
    22741 gcccgtatca ggcgaactgg ttctggagtc tttcgctaaa agactggcgg cgctacggcg
    22801 atattatgct ggcgtcactg gtggcgaacg tgctggcgct ggcggcgatg attttctcga
    22861 tgcaggttta cgaccgcgtc gttccggcgc agtcctaccc gacgctatgg gtgctgtttg
    22921 ccggggtgat gatggcgatc ctgtttgagt tctgtatgcg catggtgcgt acgcatctct
    22981 cggatgtgat cggcaagcgg gcggatctgc gtatttcgga tcgcgttttt ggtcacgcat
    23041 tacggctgaa aaacaacgtg cgatcgaaat ccaccggctc gtttatctcg cagatccgcg
    23101 aactggaatc ggtgcgggag ctgattactt ccaccactat cggcgcggtg gcggatctgc
    23161 cgttcttcct gctgtttgtt tttattctgt ggatgattgg cggctggctg gtgctggttg
    23221 ttttgctggc gctgccgtta ttggtgatcc ccggcctgct ggtgcaacgc ccgctggcgc
    23281 ggctggcgaa cgaaggaatg cgcgagtcag cggtacgcaa cgctacgctg gtggaagccg
    23341 tgcagtcgat tgaagatatt aaactgcttc gcgctgaaca gcgttttcag aatcagtgga
    23401 atcacaccaa cgacgtggcc tcgtccatca gcatgaagca acgttttctc accgggctac
    23461 tgctcacctg gacccaggaa gtgcaatcca tcgtctacgt cgtggtgctg ttggtgggct
    23521 gctttatggt gatgaacggc gacatgacca ctggcgcact ggtcgggacg acgatcctgg
    23581 cttcgcgcac catcgcgccg ctgtcgcaga tatccggcgt gctctcgcgc tggcagcagg
    23641 cgaaagtagc acgtaacggc cttgatgaac tgatgaagcg ccccgtggat cagccggaac
    23701 atggcaaact ggtgcataaa gcggtgctgc acggtaatta tcagttcagc aacgcggtgt
    23761 tttattacga cgaagaagag aaaattgccg atgtggcgat cggtaaactc aacattcagg
    23821 ccggagagaa aatcgccatt ctcgggcgca acggcgcggg taaatcgacg ctactgcaaa
    23881 tgctcgccgg aatgcgtatc gcccagcagg ggcaggtgtt gctggataac atcagtatcg
    23941 ggcagcttga tccggcagat ctgcggcgag acatggggct actcagccag accgggcgac
    24001 tgttctttgg ttcgctgcgg gaaaacctca ccatggggat gccggaagcc agcgatgaag
    24061 atatcgaacg cgcgttaacc ctcagcggag cgctgccctt tgtgcagaag cagaaaaacg
    24121 gcctcaacta catgattcag gaagggggat ttggtctttc tggcggtcag cgacaaacgc
    24181 tgctgctggc gcggctactc atctctcaac ccaatatcgt cctgcttgat gaacccagcg
    24241 cctcgctgga tgaaatggcg gaagcgtatt taatcgagca actgaaacag tggattgggc
    24301 atcgcactct gatcgtcgct acccaccgca cggcgatgct gcaactggtg gataggataa
    24361 tcgtgatgga tcaggggcgg attgtgatgg acggcgcgaa agaggccatt ttgcgtgaac
    24421 agggcgaacc cacggcgcga cgggtggtgt tgcaggaaaa gaataagggg tctgccgcat
    24481 gaacggacaa ctgcaatata accgtggcct gactgaaccc cgcttgccgc gttcggcgct
    24541 ggcggtacgc gttaccgctg tgatgctgct ctgctttctt ggctgggcgt ggtacttcca
    24601 gcttgatgaa gtcaccaccg gcagcggcac ggtagaaccc tccgggcgcg agcaggtggt
    24661 gcagtcgctg gaaggcggaa ttttgtatca tctcgacgtc aaggttggcg atattgttga
    24721 acagggccag ccgctggcgc agcttaaccg caccaaaacg gaatccgacg tccaggaagc
    24781 gatgtcgcgg ctgtacgctg cgctggcaac ctcggcgcgg ctgcgggcgg aagtgagcaa
    24841 taaaccgctg gtcttccctg acgaactgaa taaattcccc gaacttatcg agtcggaaac
    24901 ggcgttgtac aacactcgcc gcgacgggtt gaacaaagcc accaccgggc tgacgcaggg
    24961 gatcagtctg gtcaatcgcg aactggcgat gacccagccg ctggtaaaac agggggcagc
    25021 gagcagcgtc gaagtactgc gcctgcaacg ccaggccaat gaactggaaa acaaactcag
    25081 cgacgtgcgc acgcaatact acgttcaggc gcgggaagag ttagcgaaag ccaatgcgga
    25141 agtggaaacc cagcgttcgg tgatccgtgg gcgtgaagac tccctcacca ggctcaactt
    25201 tactgcgcca gttcgtggca ttgttcagga tattgacgtc accacggttg gcggcgtcat
    25261 cgcccccggt ggtaaactga tgaccatcgt gccgctggat gagcaactgc tgattgaggc
    25321 aaaaatctcc ccgcgcgatg tggcgtttat ccatccgggg caaaaatcgc tggtgaaaat
    25381 aactgcctac gattactcca tctacggcgg tctgccggga gaagtagcgg tgatatcacc
    25441 agacaccgtg caggatgaag tgcggcgcga tgtttattac tatcgggtgt acattcgcac
    25501 tttcagcaac catctggaaa acaaaagcaa acagcagttt ccgatattcc ccggtatggt
    25561 ggcgacggtc gatatccgca ccggcaaaaa aagcgtgctg gattatctgt tgaaaccctt
    25621 taacaaagca caggaggcgt tgcgcgaacg atgaacatta gcgatgtagc aaaaattacc
    25681 ggcctgacca gcaaagccat tcgcttctat gaagagaagg ggctggtgac gccgccgatg
    25741 cgcagcgaaa atggttatcg aacctacacg cagcagcatc tcaacgaatt gaccttactg
    25801 cgccaggcgc gccaggtggg ctttaacctg gaagagagcg gcgagctggt gaatctgttt
    25861 aacgacccgc agcgtcacag tgccgacgtc aaacgtcgca cgctggagaa ggtggcggag
    25921 attgaacggc atatcgaaga actgcaatct atgcgcaacc agctactggc gctggcgaat
    25981 gcctgccccg gtgatgacag cgccgactgc ccgattatcg aaaatctctc cggctgctgt
    26041 catcatcggg cggggtgaaa atcagttaat tcccgtttgc accgaaagaa tatgctgcaa
    26101 actttctacc ataatgcgcc ccgactgaaa taagccttcc tggatttcgt tgttaacatt
    26161 cacagcgtga accagggagg ctaataatac tacttgcaag aaggcaaaaa taaacgcacc
    26221 gataatcata atatgcgtca ggggtatttt cctttgctcc ttcattttaa ataacccaca
    26281 aactggcctt ctttcattaa ggcgactttt cctgtgtgat gataaacaat gacgttatac
    26341 agacctgact gataaacagt ggcatcttta ttgtcttcat caattgcaca taatgaatca
    26401 ttgagataag caaagttttt actgctcatt ctaaaggtca tgccaaatcc tttataaatg
    26461 gctttctttg ccgcctgggc atgttgtgcg gcttctgcat ttttaaatgc ctgcgcttcg
    26521 gaagcactta aaatagccgt ctggcgctgc tgttctgcga gataacaggt gtcatggctg
    26581 acgccctgcg cctggcaatc tgcgatgcgg gaagcgggtg agctacagcc agccagtaat
    26641 ataaccgcca gcagggaaaa cgatttaatg gagctattca tcattgttaa ccttaaataa
    26701 actgcggaat attgctatac ccgtcatact tcaagttgct gcaactcgaa ttatttagag
    26761 tataataaga aattgctatt aaaattagaa cgaatacccc acgccgacac ccgcgccgaa
    26821 atcagattgt gtatccgcag agactcccgc tttaattacc gtatgaattc cagcattaaa
    26881 cgaagcaccg acagataccg cagattcatt gttgaatgta cccgccccgg cagacaccat
    26941 cataaaatca cctgtcttaa cttgaggctg tgaagcaata gcgacggctg acgcaatacc
    27001 cgcattagcc tctttacggt tatcttccac ttcgtgtttc aacgaactga agttgttatt
    27061 gagcgaattg atttgctggc tgttatctgc aacacgtttt tgcgtgtttg ttaactgctg
    27121 gtgcgtttgg gcagaatctt gtgtcagact ttcgatttgc tcatgtgcct gggccgagtc
    27181 ttgcgcaagg tttcgaattt gttcaccgta ttgcacgcta tcgatttgct gttgcgtcgc
    27241 gacattggaa gccagttcat taattctctg ctcctgatca gcggttgaac gttcattggc
    27301 ttcattttgc gccgtcagtt tggcattaat catcttttga taatgcgcag ttgactgggg
    27361 atcgccgatt gcagcgaccg ttttttctgt ggctgcgatc tgcttctgtt gctctgtaat
    27421 ctgcgtttgt tgggcttcag actgtgattg ttgcgcatca gcagtggctg tctgaagagc
    27481 gttaatcatg ctctgataac gagcagcggt ctttacatca cccgtggcgg caacggtttt
    27541 ttgcgtggca gtgatttgtt cctgctgttc ggtaatcgcc gtactgtttt tttcaatctg
    27601 gtttttgttg tcgctgacga caccggaaag tttctggcca ttcatcagcg cggtattagc
    27661 attcgcgttg gcatgatcgg cacttgcttt aacatctgcg acatcatctt tcattgcact
    27721 cacatcggtg cggacagcgg caacatcttt ttgtgtgtcg gaaatggccg acttattggc
    27781 gtcgatatcc gtttgcgcca ccataatggc atggtgcgcg tcatcggctt taagcgaagc
    27841 ccagttagca gagtgctggg catcctgaat tgacgagtcc tgtgcggcat cgcgatgtat
    27901 ttggttctgc tcacttaacg ccatctgttc ctgctgttca gcaatagagg agctgttttt
    27961 ttcaatctgg tttttgttgt cgctcacggc accggagagt ttcaggccat tcatcagggc
    28021 ggtattggcg tttgtgttgg catgagcggc gcttgcttta acatctgcga catctttctg
    28081 agtatcagca atagctccct tgttcgcatc aattgcagat tgcgcagtgg taagcgcttt
    28141 tttggcgtca tctgcatttg ccgccgcaat gcttgcgata ttgtgtgcgt tctgaattgc
    28201 ggtatcttgc gccgcgtcgc gatcagcctg tttttggtcg gtgatgttgc tgtcagtctg
    28261 tacggctgta ctggcagggt aaatattatc gcctgtctgt gggtccatat atccatgaga
    28321 aaacaggtaa tcttgcgcgg cttctgtatt acctgtccga atcatgaagc gattgatttt
    28381 ttgatcgatt tcatcatgag acatacctgg gtgaatcgct gtcagttctg tcgctgccgc
    28441 ttcaaccgta tcatctgcaa aagcagatgg gctcacaatg gtagcaacaa cgactgccag
    28501 taaagacaac tttacagatt tcatttattt tttctcgatc aaattaagtt tattttggtt
    28561 tctaatagaa actaataata tttctattgg agttaatttg cgttgcaatt taatcgaaat
    28621 ctacgccgcc atttgtgtga tgtaaagcgc gtttttgtct tgtgtaagcg taaacactgt
    28681 ctataagaaa ctgaattggt ttcttaagta aataattttg aaaatcattt tgtgctattt
    28741 tagcgtaaaa aagagtaaag acttgttttg ataagcgaaa taaattctgt ttctggacta
    28801 aacgtgaagc ggttcctgat gtgtgctaaa atgcgtttct ttgataaatc tcaattttta
    28861 agaggtttac gccaggcagg aaaatttaaa tgaatgaatt tgagaagatt tttaatgaaa
    28921 tgaatctgga ccgggcattg ctaccgatcc tttttcgttc gaaccggtcg acagtgtgga
    28981 agtatttaag cggcgatagt acggctcctg cctctgccat gtcgctgatt atgctgttgc
    29041 agttgatcca gaaacgaaac cccgatctgt tagccgaatg gttaacgctt tctgatttca
    29101 ccattccgcc agaagtttac ctggaccagc ctgattactg gaaagggtgg gtgtatacgc
    29161 aacacaaggt caataaaaac gtcctggaat atctaaaaaa acactatccg gatgaagacc
    29221 aaaaaggtat gggtaaaacc acagaagagt aaattgtcat tacccgccca catgccggat
    29281 gcgcacagac tggcagtgtt agtaggcatg ataatacgcg tcagcgtcgc atcaggcatc
    29341 ggcgcataac cgccggatgc ggcgtaaacg ccttatccgg cctacgattc gggcgcagac
    29401 ttccaggcat gataatacgc gcccgcctcg catcaggtgt cggtgcacga ctgccggatg
    29461 cggcgcaagc gccttatctg gcctacgatt cgggcatatt ttgtaggcat gataagacgc
    29521 gtcagcgtcg catcaggcga tgaccgcacg gatgatcagc gttatccctt ctatcgcaat
    29581 gacttcaaca tgcgtacctg cgccgagatc ctcgctggcg ctgacaggcc atgaactgtc
    29641 accgacgcgc atatgaccgc gcccgttgac cagcggagat tccagcacaa aacgtcggcc
    29701 aatcagctgc tgcccgcgct ggtttaaatg actgtcgctg tgcttttgtt cgcgcacccg
    29761 ccgcgacaac catttccacc acagccaggc ggcgagcagc gtcaggacgg caaacatcac
    29821 cccttgccac tcccaaccca gcggcaccag ccagaccacc aggccggtaa tcaccgctgc
    29881 cacgccactc cacaacaaat aaccatttcc gcccagcatc tcggctgcca gcagcaaacc
    29941 gccgagactg agccagaaaa tatgtggatg aacgaccatt aactccatca tggctgagtc
    30001 cgtttgttgg cgctgtcttt caccagctca gcaatcccgg caatcgaccc catcaggctg
    30061 ctggcctcta atggcatcat cactactttg ctgttactgg acgaaccgat ctgctgtaac
    30121 gcttcggtgt atttctgcgc tacgaagtag ttcaccgcct gaatatcacc ggaggcgatg
    30181 gcttcagaca ccattttggt ggcgcgggct tctgcttcag cggaacgttc acgcgcttca
    30241 gcctgtaaaa acgccgactg acgttcgcct tccgctttca ggatttgcga ctgtttttca
    30301 ccttcggctt tgaggatttc cgcctgacgg atcccttccg cttcaagaat gtaagcgcgt
    30361 ttggtacgtt ccgctttcat ctgcgcgttc attgaagaga taagctctgc cggtgggcgc
    30421 acgtcgcgaa tttcaatacg ggtgacttta atcccccacg ggttggtggc ttcatcgaca
    30481 atacgcagca ggcgtgagtt gatgctgtcg cgctgagaga gcatttcgtc aagttccatt
    30541 gaacccagca cggtacggat gttagtcatg gtcaggttga tgatcgccag ctccagattg
    30601 ctgacttcat aggcagcgcg tggcgcgtca atcacctgaa taaaacagac tgcgtcgatg
    30661 gtaacgttgg cgttatcttt cgagataact tcctgggaag ggatatcgag cacttgctcc
    30721 atcatattga tcttgcgacc aatgcgatcc ataaacggca ccaccagact gagccccggc
    30781 tgtaacgttt tggtatagcg accaaaacgt tctaccgtcc attgatagcc ctgcggcacg
    30841 attttgacgc ccgcgccgac aatgaccagc gcgacaaaaa tgagaatcgg gataaagata
    30901 agcatcggaa aaacctcctg ttgtaccgtc cataatcagc aaaattgctg cttgattaaa
    30961 caaattatac ctgattactg aaagagagtt cccccttatt cctgcgaagg ataaactgtt
    31021 tttagtaaaa atcagaaaaa gggaacagcg atgcaggaaa atagtccttt gcttcagcta
    31081 caaaacgtag gatatctggc gggtgatgcg aagattctta ataacatcaa tttttcgctg
    31141 cgtgctggcg aatttaagtt aattaccggt ccttctggtt gtggcaaaag tacgctgcta
    31201 aaaatagttg cttcattgat cagcccaacc agcggaacgt tactgtttga aggtgaggat
    31261 gtcagcacac taaagccaga aatctaccgc caacaagtct cttactgcgc ccagacaccg
    31321 acgctgtttg gcgatacggt atacgataat ctgatctttc cctggcagat ccgtaaccgg
    31381 cagcctgacc cggccatttt tctcgatttt cttgaacgct tcgccttgcc ggacagcatt
    31441 ttgacgaaga atatcgccga gctatctggt ggtgaaaaac aacgcatctc attgattcgt
    31501 aacctgcaat ttatgccgaa ggttttattg ctggatgaaa taaccagtgc gctggatgaa
    31561 agtaataaac ataacgtcaa tgagatgatc catcgttatg tgcgcgagca aaatattgcc
    31621 gtgctgtggg tgacacacga taaagacgaa attaatcatg cggataaagt gattacactg
    31681 caaccgcatg ccggagaaat gcaggaagca cgctatgaac tcgcataata ttactaacga
    31741 atcattagca ctggcattaa tgctggtggt ggtggcaatc ttaattagcc ataaagaaaa
    31801 actggcgctg gagaaagata ttctctggag cgtcgggcgc gcgataattc agctgattat
    31861 tgtcggctat gtgctgaagt atattttcag cgtggatgat gccagcctga cattattgat
    31921 ggtgttattt atctgcttta atgcggcgtg gaacgcgcaa aaacgcagta aatatattgc
    31981 taaagctttt atctcatcgt ttattgccat tacggtcggg gcgggaatta ccctggcggt
    32041 gctgattctc tcagggtcga ttgaatttat cccgatgcag gtgatcccta tcgccgggat
    32101 gatagccggt aacgccatgg tagcggtagg gctgtgttac aacaatttag gacaacgggt
    32161 gattagcgaa cagcaacaga ttcaggagaa actgagtctt ggcgcgacgc cgaaacaggc
    32221 ttcagcgata ttgattcgcg acagtattcg cgcggcttta attccgacgg tcgattcagc
    32281 aaaaacggtt ggcttagtga gtttaccagg aatgatgtcc gggctgatat ttgccgggat
    32341 tgatccggtg aaggcgatta aatatcagat tatggtgacc tttatgctgc tctcaaccgc
    32401 cagcttgtcg accattattg cctgctattt aacctatcgt aagttttata attcgcgcca
    32461 ccagttggtg gtgacgcaat tgaagaagaa atgatgcgat gccggatgcg cagcatcgca
    32521 tccggcgttg tggttcatgt gccgatcaac ggaatatcaa tacaacaatg catacagctg
    32581 gcggcgatac ttcgacgcca gtgcatcacc cgtacccagc gcagcgagga tctcctggaa
    32641 cgttttacgc gtctgaccgt ctgcggcggt gagatcttta cgcagatgcc cgaacagcaa
    32701 ctccagcgcc tcttcattgc gcccaacctg atgcagttgc agcgccagtt gcgtcgccag
    32761 tgcggcatct tctggattct ccgccacctg ctgttgcaac tgttgaattt ccggcgtatc
    32821 agccgcctgc ttcagcagtt cgatttgcgc caccagcccc tggtagcggg tgtcctgatc
    32881 ctgcaacgga atggttttca gcaccgcttc cgcatcttca gaacggttca gcgcaatcag
    32941 cgtttctgcc agcagcaggc cgatctcccc gttctgattc gacaactgcc aggcgtcttt
    33001 cagcaatggc agggcgtcgg tgtaattgcc ttcctgcatc agttgcatcg cctgctgcgc
    33061 tttcagctct tcttcgcgcg gcagcacttt atccagcagg gcgcggatcg cctcttccgg
    33121 ttgcggcccc tggaagccat ctaccggttg cccgttctgg aacagataca cggtcggaat
    33181 cgcgcgcaga ccaaactggg cggcaatcat ctgctccgcg tcgcagtcca gcttcgccag
    33241 aataaattgc ccgttgtact gcgccgcgag gctttccaga attggggtta actgcaaaca
    33301 gtgctggcta cgttcagacc aaaaatagaa cagcaccgga gtggtcatcg actgttcaag
    33361 aacctgttgc aggttagatt cgttaatgtt gacaatattt tctacggaca tggagtcgct
    33421 ctctgttgtc gatattttct ttgacatggg ggcttaagcg cgcgcttcaa ctcacccctg
    33481 caatattttg tccatcacgc gccccggcag caggcgctta agcaccatta ctgcccaggt
    33541 caccagcgtt accggatagc gcatcttcgg cttctcgcta ataaaagcat ggcgtacttt
    33601 gtccaccacc gcttccggtc ccaacgtaaa gcgggcggcg atgccgggat tttcgactgg
    33661 tttatcactt tgcgtctggt tgacgttgtc ggtgaagcga gtacgaatgg gaccgggttc
    33721 gatcaggctg actttaattc cgctgtggcg tagctccatg cgcagcgcgt ctgaccacgc
    33781 ctccagcgca tatttactgg ccgcgtaagc gccacgaccc ggcgtggaga ttaatcccat
    33841 caccgatgat gtcatcacaa tacgcccttc accgtgcggt aacatcgcgg gtaacaggcg
    33901 catggtgagc tggtgtgcgc cgaaaaagtt ggcggaaaac tgctgttcca tctgcgcacg
    33961 gctgatggtg gaaagggggc catacatgcc gaatccggca ttgttaaaga tcccatacag
    34021 acaattatcg gtcagggcga tcacctcgtc ggctgcgcga tcaacacttt ctggtgaatc
    34081 cagatcgatc aacacgccgg taaatcccat gctgttcatg cgctcaacat catccggttt
    34141 ccggcaacct gccagcacat gaaaaccctg gcgttttaat tcgagcgcgc tttccaggcc
    34201 aattccactg gaacatccgg taattaagac cgatttttgc ataactttac ctgtcaggat
    34261 ctccgttgct ttatgagtca tgatttacta aaggctgcaa ctgcttcgcc atccagtcgg
    34321 caataaacgg ctgggcgtcg cggttgggat gaataccgtc atcctgcatc cattgtggct
    34381 tgaggtagac ctcttccata aaaaagggca gcagcggaac atcaaactct ttggcgagtt
    34441 tggggtaaat ggcgctaaag gcttcattat aacggcgacc atagtttgca ggcagacgta
    34501 tttgcattaa caatggttca gcgttggcgg ctttgacatc ctgcaaaatc tggcgcagcg
    34561 tttgctcggt ttgctgtggc tgaaaaccac gcaaaccgtc attgccgccc agttcaacca
    34621 gcacccagcg cggctgatgc tgtttcagca gagccggaag gcgcgccagc ccttgttgcg
    34681 aggtgtcgcc gctgatgctg gcattgacta ccgacgtttt actctgccac ttatcattca
    34741 acaaggcagg ccaggccgcg ctggcagaca ttcgataccc ggcgctcagg ctatcaccca
    34801 gaatcaataa cgtgtccgct gcggcggcac ggaaggttaa caggaccagg aacaggaagg
    34861 gcaaatgcca gcggaaaaca ttgttgaagt tcatcatctt aagaagtccg tcggtcaggg
    34921 ggagcatgaa ctctccatcc tcaccggagt tgagctggtt gtcaaacgtg gcgagaccat
    34981 cgcactggtg ggcgagtcgg gatcgggtaa gtcaaccttg ctggcgatcc tcgccgggct
    35041 ggatgacggc agcagtggcg aagtgagtct ggtgggacaa ccgctacata atatggacga
    35101 agaagcgcgg gcaaagttgc gcgcgaagca cgtcggcttt gtttttcagt catttatgtt
    35161 aattcctacc cttaacgcgc tggaaaacgt cgagcttccg gcgctgctgc gcggtgagag
    35221 tagcgcggaa agtcgtaacg gagcgaaagc gttgctcgaa cagttagggc tgggtaaacg
    35281 tctggatcat cttccggcac agctttccgg cggtgaacag caacgagtgg cgctggcacg
    35341 agcctttaac ggtcgacctg atgtgctgtt tgccgacgaa cccaccggta accttgaccg
    35401 ccagacgggc gataaaattg ccgacttgct gttttccctc aaccgtgagc atggcaccac
    35461 gttgataatg gtgacccacg acctgcaact ggcggcacgc tgcgaccgct gcttacggct
    35521 ggtgaacggg cagttgcagg aggaagcatg attgcacgtt ggttctggcg cgaatggcgc
    35581 tcgccgtcgc tattaattgt ctggctggcg ctaagcctgg cggtggcctg cgtgctggcg
    35641 ctgggcaata tcagcgatcg catggagaag ggcttaagcc agcaaagccg tgagtttatg
    35701 gcgggcgatc gggcgctgcg cagttcgcgc gaagtgccgc aagcgtggct ggaggaagcg
    35761 caaaagcgcg gcctgaaagt cggcaagcag ctgactttcg ccaccatgac atttgcaggc
    35821 gacacaccgc agctggcgaa cgtcaaagcg gtggatgata tctacccgat gtatggcgat
    35881 ctgcaaacca atccccctgg cctgaaaccg caggcgggca gcgtattgct ggccccacgc
    35941 ctgatggcac tgcttaacct gaaaacgggc gacaccattg acgtgggcga tgccaccttg
    36001 cggattgccg gagaagtgat tcaggaaccg gattccggtt ttaacccctt ccagatggct
    36061 ccgcgtctga tgattaatct ggcggatgtc gataaaaccg gagccgtgca gccggggagt
    36121 cgggtcacct ggcgctataa attcggcggc agcgagaacc agctcgacag ctatgagaaa
    36181 tggttgttac ctcagcttaa acccgaacaa cgctggtacg gtctggaaca ggacgaaggc
    36241 gcgctggggc gatcgatgga acgctcgcag cagttcctgc tgctttcggc gcttctgacc
    36301 ttgctgctgg cagtggcagc ggtggcggtg gcgatgaatc attactgtcg cagtcgctac
    36361 gatctggtgg cgatcctcaa aacgctgggg gcagggcgag cgcaactgcg taagctaatc
    36421 gtcggtcagt ggttgatggt gctgacgctt tcagccgtta ccggtggggc gataggcctg
    36481 ttgttcgaaa acgtgttgat ggtgctgctc aagcccgttc tgcctgctgc actaccgcca
    36541 gccagcctct ggccatggct gtgggcgctc ggcaccatga cggtcatctc gctgctggtg
    36601 gggctacgac cataccgctt gttgctggca acgcagcctt tacgcgtatt acgtaatgat
    36661 gtggttgcga acgtctggcc gctgaagttt tatctgccga ttgtcagtgt ggtggttgtg
    36721 ctgctgctcg ccggattaat gggtggcagc atgctgcttt gggcggtgct ggcgggcgcg
    36781 gtagtactgg ctttgctgtg cggtgtgctg ggctggatgc tgctgaatgt acttcgccgt
    36841 atgacgctga aatcgctgcc cctgcgcctg gcggttagcc gcctgttacg tcagccgtgg
    36901 tcaacgttaa gccagctttc ggcattttcg ctctccttta tgctgctggc actgctgctg
    36961 gtgttgcgtg gcgatctgct cgaccgctgg cagcagcagc tacctccaga aagcccgaac
    37021 tactttttaa ttaacatcgc cacagaacag gtagcaccgc taaaagcgtt cctctcggaa
    37081 catcagatag tcccggaatc gttttatccg gtggtgcggg cgagactgac ggcgattaac
    37141 ggcaagccga cagaaggcaa tgaagatgag gcgctcaatc gcgaactcaa tcttacctgg
    37201 caaaatacgc ggcccgatca taatccgatt gtcgccggta actggccgcc aaaagccgat
    37261 gaagtgtcga tggaagaggg gctggcgaaa cgcttaaacg ttgccctcgg tgataccgtg
    37321 acttttatgg gcgataccca ggagttccgc gctaaagtga ccagcctgcg caaagttgac
    37381 tgggaaagtc tgcggcctaa tttctatttt attttccctg aaggggctct ggacgggcaa
    37441 ccgcagagct ggcttaccag tttccgctgg gagaatggca acggcatgtt gacgcaactc
    37501 aaccgccagt tcccgaccat tagcctgtta gacattggcg cgattttaaa acaggtcggc
    37561 caggtgctgg agcaggtaag tcgggcgctg gaggtgatgg tggtgctggt caccgcctgc
    37621 ggtatgttgc tgttgctggc acaggtgcag gtgggaatgc atcagcgtca tcaggagctg
    37681 gtggtgtggc gcacactcgg tgcggggaaa aaactgctgc gcacgacatt gtggtgcgag
    37741 ttcgccatgc ttgggtttgt ttctggcctg gtggccgcaa ttggtgcgga aacggcgctg
    37801 gcagtgttgc aggcgaaagt gtttgatttc ccgtgggagc cagactggcg attgtggatt
    37861 gttctgccgt gcagcggtgc gctgctgctg tcgcttttcg gcggctggct gggcgcgaga
    37921 ctggttaagg gtaaggcgct gttcaggcag tttgcggggt gatgagagta aataaggggc
    37981 gttctggttg aatggaacgc cttgtttaaa ttaattctac aatatgtttc at
//
LOCUS       DAZFKF010000041        37466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFKF010000041 DAZFKF010000000
VERSION     DAZFKF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.041"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..882
                     /gene="yieE"
                     /locus_tag="ACHKA1_002492"
     CDS             1..882
                     /gene="yieE"
                     /locus_tag="ACHKA1_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO5680131.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            1087..2556
                     /gene="lysP"
                     /locus_tag="ACHKA1_002493"
     CDS             1087..2556
                     /gene="lysP"
                     /locus_tag="ACHKA1_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO5680132.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2848..4827
                     /gene="cirA"
                     /locus_tag="ACHKA1_002494"
     CDS             2848..4827
                     /gene="cirA"
                     /locus_tag="ACHKA1_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO5680133.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4859..5695)
                     /gene="yeiG"
                     /locus_tag="ACHKA1_002495"
     CDS             complement(4859..5695)
                     /gene="yeiG"
                     /locus_tag="ACHKA1_002495"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO5680134.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5953..6621
                     /gene="folE"
                     /locus_tag="ACHKA1_002496"
     CDS             5953..6621
                     /gene="folE"
                     /locus_tag="ACHKA1_002496"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO5680135.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6638..7795
                     /gene="yeiB"
                     /locus_tag="ACHKA1_002497"
     CDS             6638..7795
                     /gene="yeiB"
                     /locus_tag="ACHKA1_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO5680136.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDTQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFTQFDR
                     LGLLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            7937..8977
                     /gene="galS"
                     /locus_tag="ACHKA1_002498"
     CDS             7937..8977
                     /gene="galS"
                     /locus_tag="ACHKA1_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO5680137.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            9257..10255
                     /gene="mglB"
                     /locus_tag="ACHKA1_002499"
     CDS             9257..10255
                     /gene="mglB"
                     /locus_tag="ACHKA1_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO5680138.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10316..11836
                     /gene="mglA"
                     /locus_tag="ACHKA1_002500"
     CDS             10316..11836
                     /gene="mglA"
                     /locus_tag="ACHKA1_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO5680139.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11852..12862
                     /gene="mglC"
                     /locus_tag="ACHKA1_002501"
     CDS             11852..12862
                     /gene="mglC"
                     /locus_tag="ACHKA1_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO5680140.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(13105..14340)
                     /gene="preA"
                     /locus_tag="ACHKA1_002502"
     CDS             complement(13105..14340)
                     /gene="preA"
                     /locus_tag="ACHKA1_002502"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO5680141.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14334..15572)
                     /gene="preT"
                     /locus_tag="ACHKA1_002503"
     CDS             complement(14334..15572)
                     /gene="preT"
                     /locus_tag="ACHKA1_002503"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO5680142.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLLRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCIAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MVADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(15766..16005)
                     /gene="yeiS"
                     /locus_tag="ACHKA1_002504"
     CDS             complement(15766..16005)
                     /gene="yeiS"
                     /locus_tag="ACHKA1_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO5680143.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(16008..16727)
                     /gene="sanA"
                     /locus_tag="ACHKA1_002505"
     CDS             complement(16008..16727)
                     /gene="sanA"
                     /locus_tag="ACHKA1_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO5680144.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(16877..17761)
                     /gene="cdd"
                     /locus_tag="ACHKA1_002506"
     CDS             complement(16877..17761)
                     /gene="cdd"
                     /locus_tag="ACHKA1_002506"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO5680145.1"
                     /translation="MHPRFQTAFAQLAENLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(17891..18586)
                     /gene="yohK"
                     /locus_tag="ACHKA1_002507"
     CDS             complement(17891..18586)
                     /gene="yohK"
                     /locus_tag="ACHKA1_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO5680146.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPMILAVMG"
     gene            complement(18583..18981)
                     /gene="yohJ"
                     /locus_tag="ACHKA1_002508"
     CDS             complement(18583..18981)
                     /gene="yohJ"
                     /locus_tag="ACHKA1_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO5680147.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            complement(19112..20020)
                     /locus_tag="ACHKA1_002509"
     CDS             complement(19112..20020)
                     /locus_tag="ACHKA1_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5680148.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            20147..21505
                     /locus_tag="ACHKA1_002510"
     CDS             20147..21505
                     /locus_tag="ACHKA1_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5680149.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            21517..22545
                     /gene="gtdA"
                     /locus_tag="ACHKA1_002511"
     CDS             21517..22545
                     /gene="gtdA"
                     /locus_tag="ACHKA1_002511"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFO5680150.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            22561..23262
                     /locus_tag="ACHKA1_002512"
     CDS             22561..23262
                     /locus_tag="ACHKA1_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO5680151.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            23271..23915
                     /gene="maiA"
                     /locus_tag="ACHKA1_002513"
     CDS             23271..23915
                     /gene="maiA"
                     /locus_tag="ACHKA1_002513"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFO5680152.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            23930..25123
                     /locus_tag="ACHKA1_002514"
     CDS             23930..25123
                     /locus_tag="ACHKA1_002514"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFO5680153.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            25283..26233
                     /gene="dusC"
                     /locus_tag="ACHKA1_002515"
     CDS             25283..26233
                     /gene="dusC"
                     /locus_tag="ACHKA1_002515"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO5680154.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSHDIARAIQAIDIDKLR"
     gene            26715..27614
                     /gene="lysO"
                     /locus_tag="ACHKA1_002516"
     CDS             26715..27614
                     /gene="lysO"
                     /locus_tag="ACHKA1_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO5680155.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            27758..29410
                     /gene="hcp"
                     /locus_tag="ACHKA1_002517"
                     /gene_synonym="priS"
     CDS             27758..29410
                     /gene="hcp"
                     /locus_tag="ACHKA1_002517"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO5680156.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            29422..30390
                     /gene="hcr"
                     /locus_tag="ACHKA1_002518"
     CDS             29422..30390
                     /gene="hcr"
                     /locus_tag="ACHKA1_002518"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO5680157.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFITGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            30523..32241
                     /gene="poxB"
                     /locus_tag="ACHKA1_002519"
     CDS             30523..32241
                     /gene="poxB"
                     /locus_tag="ACHKA1_002519"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO5680158.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            32278..33279
                     /gene="ltaE"
                     /locus_tag="ACHKA1_002520"
     CDS             32278..33279
                     /gene="ltaE"
                     /locus_tag="ACHKA1_002520"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO5680159.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLNKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGIYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            33290..34720
                     /gene="ybjT"
                     /locus_tag="ACHKA1_002521"
     CDS             33290..34720
                     /gene="ybjT"
                     /locus_tag="ACHKA1_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO5680160.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGESGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLSLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            34819..35832
                     /gene="ybjS"
                     /locus_tag="ACHKA1_002522"
     CDS             34819..35832
                     /gene="ybjS"
                     /locus_tag="ACHKA1_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO5680161.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(35829..36659)
                     /gene="amiD"
                     /locus_tag="ACHKA1_002523"
     CDS             complement(35829..36659)
                     /gene="amiD"
                     /locus_tag="ACHKA1_002523"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO5680162.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     STWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMRPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(36656..36979)
                     /gene="ybjQ"
                     /locus_tag="ACHKA1_002524"
     CDS             complement(36656..36979)
                     /gene="ybjQ"
                     /locus_tag="ACHKA1_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO5680163.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(37190..>37466)
                     /locus_tag="ACHKA1_002525"
     CDS             complement(37190..>37466)
                     /locus_tag="ACHKA1_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO5680164.1"
                     /translation="NVKVAADAFLEILYSLKNKYCIKLLSAQDRAFIYEFARMIFAYI
                     NDKSIEILLLSCFAAADQKAIQRYIPQAQDGEDFRNHLQYKLTLPTH"
BASE COUNT         8781 a         9564 c        10019 g         9102 t
ORIGIN      
        1 atgcacatca ccctccggca gttggaagtt tttgcagaag tattgaaaag tggatcaacc
       61 acccaggcgt cggtgatgct ggcgttgtcg caatcagcag tgagcgcagc cttgaccgac
      121 ctggaagggc agcttggcgt gcaactgttt gatcgcgtgg ggaaaagact ggttgttaat
      181 gaacacgggc ggctgctcta tccgcgtgcg ttggcactgc ttgaacaggc ggttgaaatc
      241 gaacaactgt ttcgcgaaga caacggcgcg attcgtatct atgccagtag taccatcggt
      301 aactacattc tgcctgcagt tatcgcccgt tatcgccatg attatccgca gttgccgatt
      361 gaacttagcg ttgggaacag ccaggacgtg atgcaagcgg tgctggattt ccgcgttgat
      421 attggcttta ttgaaggacc gtgccacagc actgaaatca tttctgaacc atggctggaa
      481 gacgagctgg tggttttcgc cgcgccgact tcgccgttgg cccgtggtcc ggtcacctta
      541 gaacagctgg ccgctgcgcc gtggatcctg cgtgaacgcg gctccggcac gcgggagatt
      601 gtcgattatc tgttgctgtc acatttaccg aagtttgaga tggcgatgga attaggtaac
      661 tccgaggcaa tcaaacatgc ggtgcgtcat gggttgggaa ttagttgcct gtcgcgacgg
      721 gtgattgaag accaattgca ggcaggcaca ttaagtgaag ttgcggtccc tctgccgcgc
      781 ctgatgcgta cgttgtggcg tatacatcat cggcaaaaac acctttccaa cgcgctacgg
      841 cgctttctgg actattgcga tcccgcgaat gtcccgcgtt aagttgctgt acaagaacat
      901 gctggtgctg tgtcgatttc gtgacgcagc gccttcagca tgcattcgcc agaaaagaga
      961 ttggctgctt tacttataat ccctgggcga tcatgaaggt gtcttataac cgtgtatttc
     1021 tgccggaagg attgccaatc gtctgctaca atcgcgcctc atttttaaga tggatagcat
     1081 ttttgtatgg tttccgaaac taaaaccaca gaagcgccgg gcttacgccg tgaattaaag
     1141 gcgcgtcacc tgacgatgat tgccattggc ggttccatcg gtacaggtct ttttgttgcc
     1201 tctggcgcaa cgatttctca ggcaggtccg ggcggggcat tgctctcgta tatgctgatt
     1261 ggcctgatgg tttacttcct gatgaccagt ctcggtgaac tggctgcata tatgccggtt
     1321 tccggttcgt ttgccactta cggtcagaac tatgttgaag agggctttgg cttcgcgctg
     1381 ggctggaact actggtacaa ctgggcggtg actattgccg ttgacctggt tgcagctcag
     1441 ctggtcatga gctggtggtt cccggataca ccgggctgga tctggagtgc gttgttcctc
     1501 ggcgttatct tcctgctgaa ctacatctct gttcgcggct ttggtgaagc ggaatactgg
     1561 ttctcactga tcaaagtcac gacagttatt gtctttatca tcgttggcgt gctgatgatt
     1621 atcggtatct tcaaaggcgc gcagcctgcg ggctggagca actggacaat cggcgaagcg
     1681 ccgtttgctg gtggttttgc ggcgatgatc ggcgtagcta tgattgtcgg cttctctttc
     1741 cagggaaccg agctgatcgg tattgctgca ggcgagtccg aagatccggg gaaaaacatt
     1801 ccacgcgcgg tacgtcaggt gttctggcga atcctgttgt tctatgtgtt cgcgatcctg
     1861 attatcagcc tgattattcc gtacaccgat ccaagcctgc tacgtaacga tgttaaagac
     1921 atcagcgtta gtccgttcac cctggtgttc cagcacgcgg gtctgctctc tgcggcggcg
     1981 gtgatgaacg cagttattct gacggcggtg ctgtcagcgg gtaactccgg tatgtatgcg
     2041 tctactcgta tgctgtacac cctggcgtgt gacggtaaag cgccgcgcat tttcgctaaa
     2101 ctgtcgcgtg gtggcgtgcc gcgtaatgcg ctgtatgcga cgacggtgat tgccggtctg
     2161 tgcttcctga cctccatgtt tggcaaccag acggtatacc tgtggctgct gaacacctcc
     2221 gggatgaccg gttttatcgc ctggctgggg attgccatta gccactatcg cttccgtcgc
     2281 ggttacgtat tgcagggaca cgacattaac gatctgccgt accgttcagg tttcttccca
     2341 ctgggtccga tcttcgcatt cattctgtgt ctgattatca ctttgggcca gaactacgaa
     2401 gcattcctga aagataccat tgactggggc ggtgtagcgg caacgtatat tggtatcccg
     2461 ctgttcctga ttatttggtt cggctacaag ctgattaaag gaacccactt cgtacgctac
     2521 agcgaaatga agttcccgca gaacgataag aaataagttt ctcccttcct tgctaagccc
     2581 tctcaaccga gagggctttt tcaattccat ttccctgaca aatcatgcgg atataaaatt
     2641 taacatttgg attgataatt gttatcgttt gcattatcgt tacgccgcaa tcaaaaaagg
     2701 ctgacaaatc agaggctggt ccggttttct gggatgatca cctgcataca aataagtcca
     2761 ccgcgatgct gccgtacgca aggggacgtg aagaagatgt gagcgataac ccattttatt
     2821 ttcgtagtta cctcatggag atatggaatg tttaggttga accctttcgt acgggtcggg
     2881 ctgtgtttgt ccgctatttc ttgtgcatgg cctgtgttag cggtcgatga tgatggcgaa
     2941 acgatggttg tcactgcatc ttccgttgaa caaaacctta aagatgcacc cgccagtatc
     3001 agcgtcatta cccaggaaga cctgcagcga aaaccggtac agaatctgaa ggatgtcctc
     3061 aaagaagtgc ctggcgtaca actgacgaac gaaggggata accgtaaggg cgttagtatt
     3121 cgtggtctgg acagcagcta caccctgatt cttgtcgacg gtaaacgcgt taactcccgc
     3181 aatgccgtct tccgccacaa tgatttcgat ctgaactgga tcccggtcga ttccatcgaa
     3241 cgtattgaag tggtccgtgg cccgatgtcg tcgctgtacg gttccgatgc gctcggcggt
     3301 gtagtgaata tcatcaccaa aaaaatcggt cagaaatggt cgggtaccgt taccgtcgat
     3361 accaccattc aggaacatcg cgatcgcggt gatacctata acggtcagtt ctttaccagt
     3421 ggaccattaa ttgatggtgt gctgggaatg aaagcttacg gcagcctggc aaaacgtgaa
     3481 aaggatgacc cgcaaaactc aacgaccacc gataccggag aaacgccgcg tattgaagga
     3541 ttctccagcc gcgacggcaa tgtcgaattt gcctggacac cgaatcaaaa tcacgatttt
     3601 actgccggat acggtttcga ccgtcaggat cgtgattccg actcgctgga caaaaaccgc
     3661 ctggaacgcc agaactactc cgtcagccat aatgggcgtt gggattacgg caccagcgaa
     3721 ctgaaatact acggtgagaa agtcgagaac aaaaaccctg gcaacagcag cccgataact
     3781 tccgaaagca atacggtcga cggcaaatac acgttgccgc tgacggcgat taatcagttt
     3841 ctcacggttg gcggtgaatg gcgtcacgac aaacttagcg atgcggtgaa cctgaccggg
     3901 ggaaccagct ccaaaacgtc tgccagccag tacgcgctgt ttgtggaaga tgaatggcgg
     3961 atcttcgagc cgctggcgct gacgaccggc gtgcgtatgg acgatcacga aacctacggt
     4021 gaacactgga gtccgcgtgc ctacctggtt tataacgcca ccgacaccgt aacggtgaaa
     4081 gggggctggg cgacggcatt taaagcacct tctctgttgc aacttagccc tgactggacg
     4141 agcaattcct gccgtggcgc atgtaagatt gtgggtagcc cggatctgaa accagaaacc
     4201 agcgaaagtt gggagctggg gctgtactac atgggtgaag aaggctggct ggaaggggtt
     4261 gaatccagcg ttaccgtttt ccgtaacgat gtgaaagatc gtatcagcat cagccgtacg
     4321 tctgacgtca atgctgcacc gggctaccaa aactttgttg gttttacagc gaacggtgtg
     4381 ccggtattta gctactacaa cgttaacaaa gcacgtattc agggcgtgga aaccgagctg
     4441 aaaattccgt tcaacgatga atggaaactg tcactcaact acacctacaa cgatggtcgt
     4501 gatgtcagca acggcgaaaa caaaccgcta tccgatctgc cgttccatac cgccaacggt
     4561 acgctggact ggaaaccgct ggcgctggaa gactggtcat tctatgtttc tggtcactat
     4621 accgggcaga aacgagccga cagcgcgacg gctaaaacac cgggcggtta taccatctgg
     4681 aataccggcg cggcctggca ggtgactaaa gacgtcaaac tgcgcgcagg cgtgctgaac
     4741 cttggcgaca aggatctcag tcgtgacgac tacagctata acgaagacgg acgtcgttac
     4801 tttatggcag tggattatcg cttctgatga gaagatgccc ggcgaaccgg gcggactttc
     4861 acttcagtaa atactgcgca tggaagcgca ggtgatcctc tataaaagag gcgatgaagt
     4921 agtaactgtg atcatatccc ggctgaatac gcagcgtcat cggccacgct ttctggcgcg
     4981 cggcttctgc cagaactgcg ggttgcaact gatcggcaag aaactgatca ttatcgccct
     5041 gatcgataag cgtcgggatc gcatcctgcg cgttactggc atacatcagc gcgcaactgt
     5101 cccattccag ccatgcattt ttgtcctcac ctaaatagcg gctaaacgct ttgattcccc
     5161 acgggacgct gcacggattc acaattggcg caaaggcgga aacgctggtg tatttgcccg
     5221 ggtttttcag cgccataatc agcgcaccgt gaccacccat tgaatgaccg ctaatagcgc
     5281 agcggtcgct gacattaaat tgcgactgaa ccagcgccgg taattcatcg cgcagataat
     5341 catacatccg gtaatgcgtc gcccacggcg gttgcgtggc attaagataa aagcctgcgc
     5401 cctggcctaa atcgtagcca tcgtcgttgg caaccttttc gccgcgcggg ctggtgtctg
     5461 gcatcaccag tacaatcccc agttctgccg ccacgcgctg ggcacccgcc ttggtggtga
     5521 agttctcgtc attgcaggtt aatccggaaa gccagtacag cactggtggc ggggtgtgat
     5581 cacgaggggg ggggagaaag atactgaacg tcatcgggca gtttaaggta ctggagtcgt
     5641 gtcgccagcg ttgctgccag ccttcaaaac agcggtgctc ttcgagcatt tccatgcgtg
     5701 gctccttgtt gtgttgtttg caatttcttc atcataccgt ttattcaatg tgctgtgagt
     5761 aactttcact tccgtatttg cataacgatg ttttaacatc tgctgatgaa aggcagcggc
     5821 aattacaata attatcgctg tgaatactgg attatgtgcg ccgcctcacg cacaataatc
     5881 aggctgtaaa tcagcttaat aactttgccc ccacgcaggg cggaggcgtc acacctgcag
     5941 gagaaatcat aaatgccatc actcagtaaa gaagcggccc tggttcatga agcgttagtt
     6001 gcgcgaggac tggaaacacc gctgcgcccg cccgtgcatg aaatggataa cgaaacgcgc
     6061 aaaagcctta ttgctggtca tatgaccgaa atcatgcagc tgctgaatct cgacctggct
     6121 gatgacagtt tgatggaaac gccgcatcgc atcgctaaaa tgtatgtcga tgaaattttc
     6181 tccggtctgg attacgccaa cttcccgaaa atcaccctca ttgaaaacaa aatgaaggtc
     6241 gatgaaatgg tcaccgtgcg cgatatcact ctgaccagta cctgtgaaca ccattttgtt
     6301 accatcgatg gcaaagcgac ggtggcttat atcccgaaag attcggtgat cggtctgtca
     6361 aaaattaacc gtatcgtgca gttctttgcc cagcgtccgc aggtgcagga acgtctgacg
     6421 cagcaaattc ttattgcgct acaaacgctg ctgggcacca ataacgtggc tgtctctatc
     6481 gacgcggtgc attactgcgt gaaggcgcgt ggcatccgcg atgcaaccag tgccacgaca
     6541 acgacctctc ttggtggatt gttcaaatcc agtcagaata cgcgccacga gtttctgcgc
     6601 gctgtgcgtc atcacaactg attaaaaggc aggaaccatg gagcgcaacg tcacgctcga
     6661 ttttgttcgc ggcgtcgcca ttctggggat cctgctatta aacatcagcg cctttgggct
     6721 accaaaggcg gcttatctta atcccgcctg gtacggcgct attacgccgc aggatgcatg
     6781 gacctgggca tttctcgatc tcatcggcca ggtgaaattc ctcacgcttt ttgcgctgct
     6841 gtttggtgct ggcctgcaaa tgttgctgcc ccgtggcaga cgctggatcc agtcgcggtt
     6901 aacgctgtta gtcttgctgg gctttattca cggtttattg ttctgggacg gcgatattct
     6961 gctggcttac gggctggtgg gcttaatctg ctggcggctg gtgcgcgatg cgccatcggt
     7021 aaaaagcctg tttaataccg gcgtcatgct ttatctggtg gggcttggcg ttttgctgtt
     7081 attggggctg atttccgata cccagaccag ccgcgcctgg acgccggatg catcggctat
     7141 tttgtatgaa aaatactgga agcttcacgg cggcgttgaa gcgatcagta atcgtgccga
     7201 tggtgttggc aacagtttac tggcactggg cgcacagtat ggctggcaac tggctgggat
     7261 gatgctcatt ggtgccgcat tgatgcgcag tggctggctg aaagggcagt tcagcttacg
     7321 tcactatcgt cgtactggtt ttgtgctggt ggcgattggg gtgatcatta accttcctgc
     7381 catcgccctg caatggcggc tggactgggc atatcgctgg tgcgccttct tacttcagat
     7441 gccgcgggaa ctgagtgcac cgtttcaggc gattggctat gcgtcgctgt tttatggttt
     7501 ctggccgcaa ttgagccgct ttaagctggt gcttgcgatc gcctgcgtcg gacggatggc
     7561 gctgaccaac tatctattgc aaacgctgat ttgtaccacg cttttttatc acctcggttt
     7621 gtttacgcag tttgaccgtc tgggattgct ggcgtttgtt attccggtat ggctggcgaa
     7681 tattctcttc tctgttatct ggctgcgtta cttccgccag gggccggtgg aatggctctg
     7741 gcgtcagtta actttgcgtg ctgccggacc ggcaatatct aaaacatcaa gataacgatc
     7801 tggatcacat tcgttaacaa aacggctgta accgtttcca ttgctgtgac tcgattcacg
     7861 aagtcctgta ttcagtgctg acaaaatagc cgccagcaag cagtcattta ctgcaatctc
     7921 ataacaggta gtgaatatga tcaccattcg tgatgtagcg cgtcaggctg gcgtctctgt
     7981 ggcaacggtt tcccgggtgc tcaataacag cacgctggtc agtgccgaca cgcgtgaagc
     8041 agtaatgaaa gccgtgagtg agctggatta tcggccaaac gccaatgccc aggcgctggc
     8101 aactcaggtt agcgacacca ttggcgtggt ggtgatggac gtttctgatg cgtttttcgg
     8161 cgcgctggta aaagcggtgg atctggtcgc ccagcagcat cagaaatacg tgctaatcgg
     8221 caatagctat catgaagcgg aaaaagaacg tcacgccatt gaggtgttaa ttcgccagcg
     8281 ttgtaatgca ttgattgttc actcaaaagc attgagtgac gatgaactgg cgcaatttat
     8341 ggataacatt cccggtatgg tgttaatcaa ccgcgttgtg ccggggtacg cccatcgttg
     8401 cgtttgcctg gataatctca gcggtgcccg aatggcgacg cgcatgttgc tgaataacgg
     8461 tcatcaacgt attggttatc tttcttccag ccacggcatt gaagatgacg ccatgcgtaa
     8521 agcaggctgg atgagtgcgt tgaaagagca ggatattatt ccgccggaaa gctggattgg
     8581 cactggtacg ccggacatgc cgggcggtga ggcggcgatg gttgaactgc tggggcgcaa
     8641 tctacaactt accgctgtat ttgcttataa cgacaatatg gctgctggcg cactgacagc
     8701 attaaaagat aatggcattg cgattccgtt acatctctca atcatcggtt tcgatgatat
     8761 tcccatcgcc cgttacaccg acccgcaatt aacgaccgtg cgttatccca ttgcttcaat
     8821 ggctaaatta gccaccgaac tggccttgca gggtgcagca ggcaatattg atcctcgtgc
     8881 cagccactgt tttatgccga cgttagtgcg tcgccattct gtcgcaacgc gccagaatgc
     8941 ggcggcgatc actaactcaa caaatcaggc gatgtaaccg ctttcaatct gtgagtgatt
     9001 tcacagtatc ttaacaatgt gatagctatg attgcaccgt tttaacgttg taacccgtat
     9061 gtaacagtga ataatcactt tcaccgaggt aatagcgtca taacaacaat taaagccgtt
     9121 ttctggagcg ttaccgggca tggaagaacg aattttaaaa agtgagcttc ggcgttcagt
     9181 aacacttcat taactctact gccccgccga gcatttatct caagcactac cctgcataag
     9241 aaaaaccgga gataccatga ataagaaggt gttaaccctg tctgctgtga tggccagcat
     9301 gttattcggt gccgctgcac acgctgctga tactcgcatt ggtgtaacaa tctataagta
     9361 cgacgataac tttatgtctg tagtgcgcaa ggctattgag caagatgcga aagccgcgcc
     9421 agatgttcag ctgctgatga atgattctca gaatgaccag tccaagcaga acgatcagat
     9481 cgacgtattg ctggcgaaag gggtgaaggc actggcaatc aacctggttg acccggcagc
     9541 tgcgggtacg gtgattgaga aagcgcgtgg gcaaaacgtg ccggtggttt tctttaacaa
     9601 agagccgtct cgtaaggcgc tggatagcta cgacaaagcc tactacgttg gcactgactc
     9661 caaagagtcc ggcattattc agggcgattt gattgctaaa cactgggcgg cgaatcaggg
     9721 ttgggatctg aataaagacg gtcagattca attcgtactg ctgaaaggtg aaccgggcca
     9781 tccggatgca gaagcacgta ccacttacgt gattaaagag ctgaacgaca aaggcattaa
     9841 aactgaacag ttacagttag ataccgcaat gtgggacacc gctcaggcga aagataagat
     9901 ggacgcctgg ctgtctggcc cgaacgccaa caaaatcgaa gtggttatcg ccaacaacga
     9961 tgcgatggca atgggcgcgg tagaagcact gaaagcacac aacaagtcca gcattccggt
    10021 gtttggcgtc gatgctctgc cagaagcgct ggcgctggtg aaatccggtg cactggcggg
    10081 caccgtactg aacgatgcta acaaccaggc gaaagcgacc tttgatctgg cgaaaaacct
    10141 ggccgatggt aaaggtgcgg ctgatggcac caactggaaa atcgacaaca aagtggtccg
    10201 cgtaccttat gttggcgtag ataaagacaa cctggctgaa ttcagcaaga aataagactg
    10261 attgggcgca gtctattact gcgcccttat tgtatggcca acaaggtaaa attatatggt
    10321 cagctcaacg actccgtctt ccggggaata cttgttggaa atgagcggta tcaacaagtc
    10381 ctttcctggt gttaaggcac ttgataacgt taatttaaaa gtccggccac attctatcca
    10441 tgcattaatg ggggaaaacg gtgcaggaaa atcgacatta ttaaaatgcc tgtttggtat
    10501 ttatcaaaaa gactccggca ccattttatt ccagggtaaa gagatcgatt tccattcggc
    10561 caaagaagcc ctggaaaatg gtatttcgat ggtacaccag gagttaaacc tggtattaca
    10621 acgttcggtg atggataaca tgtggctggg gcgatatccc accaaaggca tgtttgtcga
    10681 tcaggacaaa atgtaccgcg aaaccaaagc gatttttgat gaactggata ttgatatcga
    10741 tccgcgtgcg cgcgtcggca cattatctgt ttcgcaaatg cagatgatcg aaatcgccaa
    10801 agcgttttcc tataacgcga aaattgtgat tatggatgaa ccgacttctt cgttaaccga
    10861 aaaagaggtc aatcatctgt tcactattat tcgtaaatta aaagagcgcg gctgcggtat
    10921 tgtttatatc tcgcataaaa tggaagaaat cttccagtta tgtgatgaag ttaccgtatt
    10981 gcgcgacggt cagtggatcg ccaccgaacc gctggcagga ctgacgatgg ataagatcat
    11041 cgccatgatg gttgggcgtt ctcttaacca gcgtttccct gacaaagaaa acaagccggg
    11101 cgaagtcatc ctcgaggtgc gtaacctgac gtcactgcgc cagccgtcga tccgtgatgt
    11161 ctcgtttgat ctgcataaag gggagatcct cggtattgcc ggtctggtgg gggcgaaacg
    11221 taccgatatt gttgagacgt tatttggtat tcgcgagaaa tcggctggca ccattacgtt
    11281 gcacggcaaa aagatcaata accataatgc caacgaagcc ataaaccacg gatttgcact
    11341 ggtaactgag gagcgccgct caacaggaat ttatgcctat ctggatattg gttttaactc
    11401 gttaatttcc aatattcgca actacaaaaa taaagttggt ttactggata actcgcggat
    11461 gaaaagcgat acccagtggg tgattgattc gatgcgggta aaaacaccgg ggcatcggac
    11521 gcaaattggt tcgctctccg gtggtaatca gcaaaaggtg attattgggc gctggctact
    11581 aacgcaaccg gaaatattaa tgctcgatga accgacgcgc ggtattgatg tcggggcgaa
    11641 gtttgagatc tatcagttaa ttgccgaact ggcgaagaaa ggcaaaggga ttattattat
    11701 ctcctctgaa atgcctgagt tgttagggat aacagaccgt attctggtca tgagcaatgg
    11761 tctcgtttcc ggaattgtcg atacaaaaac aacaacgcaa aacgaaattc tgcgtcttgc
    11821 gtctttgcac ctttaagatt aggggcttcc catgagtgcg ttaaataaga aaagttttct
    11881 tacttacctg aaagagggcg gtatttacgt cgttctttta gttttgctgg cgattattat
    11941 tttccaggac ccaacatttt taagtctgtt gaacttaagt aatattctca cccagtcatc
    12001 ggtgcgtatt attatcgcgc tcggtgtggc agggttaatt gtcacccagg ggaccgacct
    12061 ttctgcgggg cgtcaagtag ggctggcggc agtggtggca gcgacattat tgcagtccat
    12121 ggataacgcc aacaaagtgt tcccggaaat ggcgacgatg ccgattgcgc tggttattct
    12181 gattgtctgt gccattggtg cggtgatcgg tttgatcaac ggtctgatta tcgcttatct
    12241 caacgttacg ccgttcatta ccacgctcgg cacgatgatc atcgtctatg gcatcaactc
    12301 gctctattac gatttcgtcg gggcgtcgcc aatttctggt tttgacagtg gcttctctac
    12361 ctttgctcag ggctttgtcg cgctggggag tttccgtctc tcttacatca ccttctacgc
    12421 gttgattgcg gtggcgttcg tctgggtgtt gtggaacaaa acccgcttcg gtaagaacat
    12481 ttttgccatt ggcggtaacc cggaagcggc aaaagtatct ggtgtcaacg tcggcctgaa
    12541 cctgctgatg atctacgcgt tgtctggcgt gttctatgcc tttggcggga tgttagaagc
    12601 cggacgtatc ggctctgcca ccaacaacct cggctttatg tatgagctgg atgctatcgc
    12661 ggcgtgcgtg gtaggcggcg tatcgttcag cggcggtgtg gggacggtga ttggcgtggt
    12721 gaccggggta attattttta ccgtcatcaa ctatggcctg acgtatatcg gcgtaaaccc
    12781 atactggcag tacatcatca aaggggcgat tattatcttc gccgtagcgc tggattcact
    12841 gaagtacgcg cgtaagaagt gatatgattg tggtccgcat tcggtcataa acgcacataa
    12901 catcatgatc tgtgtggttt tgtaggccgg ataaggcgtt cacgccgcat ccggcgacag
    12961 gagccaaatg ccggatgcaa cgctggcgcg ttttatccgg cctacaatga tctcaacata
    13021 ctgatttgta tggttttgta ggccggataa ggcgttcaag ccgcatccgg cgacaggaac
    13081 caaatgccgg atgcgtctct ggttttacaa cgttaccggg tgttctttct cgcctttctt
    13141 aaacttcact tccccgagct caatacaacc caccgggcag acgtgaccac aaagcagaca
    13201 acccacacat ttctcggtat tacaatgcgg tgtgcgggtt ttctcgctcc attccatcgc
    13261 ctggtgaccg ccgtcgtaac aggaaatata acagcgtcca cagccaacac atttatcaag
    13321 attgatacgg ggatagacaa tataactgcg gtctaaatct tccgccggga caatattgtt
    13381 attcgccaga cctaccatct cctgcaacga atcaaatccc tgatcggcga gataatgcga
    13441 caacccgctc gccatatctt ccactatccg atacccgtac tgcataatgc cggtggtcac
    13501 ctgtaacgtt gctgcgccga gcaataaaaa ctccgccgca tcctcccagg tttcaatgcc
    13561 gccgataccg ctgattggga aatcgcgcag ttctggatgg gtgcgcattt gctgaatgaa
    13621 gcgcagggcg atcggtttta ccgctttacc ggaatatccg gaaatactcg attttccgtt
    13681 aacgatcggc ataccgattt tctgattaag atcgatattg gtgatggatt taacggtgtt
    13741 aatcgccgca atgccatctg cgccgccgcg cttcgccgcc agcgccactt cgcacatatc
    13801 accgatattc ggcgtcatct tcgccagcat tggcagcgtg gaaccccgtt tcactgcccg
    13861 acaatatttt tctaccagct ccgggctttg cccgacatcg ctacccatcg catgagaagt
    13921 catttgcgga caggagaagt tacactcgat catatccgcg ccagcttctt gcaccaggcg
    13981 cgccagctcc tcccattgct gctcattttc ccccatgatc gaagcgatca atactttgtc
    14041 cgggtaatct tccttcagcc gacgcagggc ggccagattc tcttccaacg gatgttcagc
    14101 aatctgctcc atatttttga agccgataaa accggtatct tctttcacca gatgatcaaa
    14161 acgcggcgag acttcgttgg cgataaaaaa gccgatcgtt ttaaacacca cgccgcccca
    14221 acctgtgtcg taggctttgg cacacatctc atagcagttg cctaccggcg aagaagagag
    14281 gcagaacggg ttgggaaact tcacgccgca aaaagtaatc gaaagatctt tcgttaacat
    14341 gagcaagctc cctctaaata gtgatgaatc gcctcggcgg cttctttccc ggttttcacg
    14401 gcatagacca cggttttgtc accctcaaca atatcgccag cagcaaagac ttgcggatcg
    14461 cgggtctgat aattttgtgt tttgatggtg ttacgctgcg gctctaactc cgcaaaggca
    14521 tccagtctgg catgctgacc gacggcgaga ataattttat ctgccaccat cgtcagttcg
    14581 cccgataacc gtacatgctt aaacgtgact ttattgcctt ccacggctac tggcgtgaat
    14641 ccatcgatga tcgaaacgcc cagctcccgg gcgctggcaa actctttttc gcttgccgga
    14701 aactcatcta actcttcacg cgctatgcag gttaccgcct gacagccgag aactttcagc
    14761 gtgctggcta cgtccatcgc gacatcaccg ccgccgataa ttaatgcgct ttgtggaatg
    14821 ctgatatcgc cttgcgcctg tcgtgcacgt agcaggaagt cgacggcaat ctcaacgtcg
    14881 ctatgctcaa acagcggtag cccggaaccg cttgataacc ccacggtgac cagtaccgcg
    14941 cggttttccg ctttcagttg ctccagggtg agtgttttac cgatttcgtt attgcactta
    15001 atggtcacgc ccattttttc aatacgggcg atctctgcat ccagcactga ttgcggtaac
    15061 cggaattgcg gaataccgtt acgcaaccag ccaccggggt gcgcttcttt ctcataaatc
    15121 gtgacgtcat aaccctggtt tgtcagtgtc acactggcct gcaatccggc aggacctgcg
    15181 ccaataatcg cgactttgcc gagcgtttta gtaccaggct gataaatttc catccctgtt
    15241 tgttgttcaa aatcagtaac aaaacgctgt aagcggccga tatcaatggg agcatcaaca
    15301 ccggcacggg tacaaccgct ttggcataat ttctccgtcg ggcacactct ggcacaaacg
    15361 gcaccgaggg cattattttc gcgaattgtc tcggcagcgc ctttaaaatt acgaaagtag
    15421 attgagcgaa taaatttccc cggatcggtc tgtgccgggc aagcctgact acagggagcg
    15481 tcgtgacata ataaacagcg agaggcttct ttaatcgcca gtaaaggcgt aaaagccgga
    15541 gtgagttcat ccagataatt ttgttgcggc atgtgatctt ccttaatgct atgagtaatt
    15601 aaggcgaacg ctaaataaat attgcgtgct gatattaagg ctatgcgcgt ggggaaattt
    15661 aaactgtgaa aggattcaca aatagctgcg gttcagaatg ttatatccgc agcttattga
    15721 gtgaagtaat aattattctc aataattaac agaaaagaga aaatattaac gatagaaaat
    15781 aagataaaca atcatgccga tagataaaat tccgaagccg atatatgcca ccatataagc
    15841 gaaaaagagt ccgggatttt ttgctcgcca gacatacacc ggttccggtg catttttaac
    15901 ccgtttatca atcgcgcctg cgcgccagcc tttccatgct tcgcggaaac aaaatatcgg
    15961 gataaggaaa aaaacggcaa cgacaaaaaa ctgctgtaca tccataacta ctttccttgt
    16021 ttcttttgta attcaagtaa ctgttcgggt gtgacggcgg gatacccctg cgcatcttcc
    16081 ggcacctggt gcatagccgg aatagggacc agcggcccta aaaaacgcgg ttcacgttta
    16141 aaaatataaa ggtcagccag cgcaccgaaa cgggcggcaa attcgcgaat acgtactgac
    16201 agcatatctt tcggtgacgg tacggcataa cactgagcct gaatccccat atgcagcgca
    16261 ataaataatg cccgctcaca gtggaaacgt tgggtgataa taatgaaatc attagtatcg
    16321 aaaactttgc gtgtacgcac gatggaatcc agcgtacgaa agcctgcgta atcgagaaca
    16381 atatctgatg ggtcgacacc agcagcgatt aaatctttgc gcatggtcat cggctcatta
    16441 taactttgca atgcgttatc gccgctcagt aatagataat ttaccttacc gctgttatag
    16501 gcattaatcg ctccttgaat gcggtagcga taatactgat taattacgcc agtacgataa
    16561 tattttgctg ttccgagcac cacaccgacc tggcggtagg ggagatcctg caattcgtcg
    16621 tagatataag gcgcggtttt ccagctcatc cagcgatcga ggccgagcac agtcaacagc
    16681 agcaagccga taaggactaa caggctgagg aacacgcgct ttaacatgca ggagactcga
    16741 tctaaggaaa atttccatca ggctacttta cccgctggtt ttgcgcaaga aacggcaaac
    16801 tgtttgccgg gattttcgac accaggagag ggctggcaag ccggacaagg cgttcacgcc
    16861 gcatccggca ccaggcttaa gcgagaagca cgcggtcgat actgtgacag ccgagcgctt
    16921 tcagcgttgc ggaggtggca tcccactgaa tcaacggcgc atcggctttt tctgccagaa
    16981 ccgcgcgctg gatatccggg taatcataac ccttgagatt caacagaatt aacgctccct
    17041 gcaacggcgg cagagtcggg ttgaatgcgg cgttttcagc gtagctgccg ctgaaaattc
    17101 gaccgtcttt acattccagc gcaacaccgc ttggcgactt actgtaaggc atgtgcgaac
    17161 ggtttgccgc cgcaatcgcc gcctgagaaa gcgcatcacc agtcagcgca tagccgtgat
    17221 cctgctcgtc catcagcagc gttttaatct ccagatcttt cggcccaaag gcatccggca
    17281 gatagtcacg cagcgcgtgt gcctcgcggc ccggcagatg aatacgcaga tccagaccgc
    17341 tgttcagttc attcataaac tgacggcagt gaccacaagg cgtgtagtta acggtgatgg
    17401 ctgcaagcgc tttttcacca ctcaaccagg cgtggctgat cgcgctttgt tcggcatgaa
    17461 cggtttgctg cattgtcgca ccaataaatt ccatattggc accaaaatac caggttccgc
    17521 tcacaccgcg cgcaattgcg ccaacattaa aattcgacaa tggcgtacgc gcacaggccg
    17581 ccgccagcgg aagtagtgcg aatgccagcg cgtcttcgtc cagccccgtt gcgctcttca
    17641 gcgatgagac ttgctccccg gtcaacaaag cggggaagta cttgtctgcc agaataggtt
    17701 ccagtgcaga ttgcaagttt tccgcaagtt gggcaaaagc ggtttgaaaa cgtggatgca
    17761 tggcgttgcc tcataatcat gtaatgggat aacagtttac gaacccgtta tggctttata
    17821 tgtgatctga atctcattaa ttatgcaacg cgtgaaactt acatcaaaaa tgcgcgacgc
    17881 atcgcaaatt ttagcccatt actgccagaa tcatcgggaa aaggaacggt gcgatcagcg
    17941 aagtaattat cccgcataac accagcgcta gcgaactaaa tgcaccttcc tgataatcca
    18001 gctcggcgca gcgcgctgta ccgagggcgt gcgaggcagt ccccattgcc agaccgcgcg
    18061 cagctttggt acgaatacgc atcgcattaa gcaatgtatg gccaaatacc gcgccgagga
    18121 tgccgacgaa aatcacgcaa actgcgctga ttgccggaat accgccaata ctgccgccaa
    18181 ccgccattgc aataggcgtg gtgactgatt tcggcaggat tgacgcggcg atttccggtg
    18241 aagcgcccat caacaatgcc acggaagtcc ccgtcaccat cgcaaccacg ctgccgataa
    18301 aacagatggt gatgatcgat ttccagcgcg cgcggatctg gtgtagctgc tcatataaag
    18361 gataggccag cgcgacgacc gccggttgca gcagatcgtt tagcacttcg ctacctttaa
    18421 agtaactgtc gtaagagatg ccggtcagca ttaaaaaagg aatgatcacc accattgcta
    18481 ccagcaacgg gttaagcagg ggaaacttat accgtgccgc cagtttgcgg gcggcaaaaa
    18541 agacaatcaa cgtcagcggt aatgaccacc agatattcgc catcattctt ctgatccttt
    18601 ctgacctacg actttacgtt caccgtgtac cagttgcgaa ctccagctca ccaccagaaa
    18661 aaccaccagc gtactgattg cacaggaaac cactaccggg ccaaactgtg cgcggagcaa
    18721 atcaaaatat tgcatgacgc ctacgccaat cggcacaaac aatagcgcca tatagcgaat
    18781 cagtacgtag caccccggat tgacccattt cgccggaaga atttgcaagg ccagcaggac
    18841 aaacaggatc agcatcccga tgatgctgcc cggaatggtt accggtagca gggaagcaat
    18901 gaaaatgcct gcatacaggc aggcataaat caggacgaaa gcgcgtaaat attgccagat
    18961 aatgttcagt gtcttactca taatcaacac cccagatgag atggattcat catacaatta
    19021 attcgtaaaa cgtgctaccg atcacattaa gaattctcag catagttgaa ttttagggag
    19081 gtaacctcgg gcgagcgccc cgttcagcac actaattctg ctgtaaaaag tgcagaaact
    19141 gacttaaagc ataagaaggc gtttcgttat tgcgccagaa cactccgacg ctacctttct
    19201 gttctatctt tggcaggttg agtatcacca gttgtccctg gctggcataa cgttgcgcca
    19261 ggcgtaatga caaaatagag atcatatcgc tgctttgtaa caggttggta ctgacgttca
    19321 tcgacgccga ctcaatcgtg ttttctggca gcattacgcc gtggtctacc agcgcattat
    19381 caatactaat gcgaatgggg gtacccgtcg gccagacgat ccagcgccag ttggcaagat
    19441 cattccagct gagggtggcg tatttcgcca gtgggtgctc agggcgggcg acaaagcata
    19501 ctggctcggt gtacagcacc tgataattca gtggcagctg caatgcgcga ccgccaattc
    19561 gcccaacgac cacatcgaca gaacctgcca atagatcttg cagcaatggg gtcatcacct
    19621 tctcttcgat attcaggtgt agagttggca tcttcgtaag caaatgcaaa atcgcctggg
    19681 atacgcagtc agtggcgacg ggagaacagc caattttcag actgccgacc agtccacctt
    19741 gtttaaaacg ggccagttca tgttgcgatc gctccatgtc attgattaac cgttgggcat
    19801 gttgcaataa caatttgccg ccttcagtcg gacgtaatcc tttgctgtgc cgctcaaaaa
    19861 gtgtgattcc aatctcatct tcgagttgtg acaaccattt cgaaagcgca ggttgggtga
    19921 tattcatcat tcgtgcgaca tgggttagat tgccctgttc acccagcgcc accagcattt
    19981 caaggtgatg tagttttaat ttttgcgccc agttcgccat ttgccataac ctccaggtta
    20041 tctctctgcc cgaaatgcca ttgtacatct catctcttta atcacaatat cgcaacatat
    20101 acaaaacaaa agaaacatct accttacctg cactgaggca tttactatga ctcaacgacg
    20161 tgatttacaa gcacttatcg atgctgctcc ggtcggcaag atgcagtggc gcgttatcat
    20221 ctgctgtttt ctggtggtaa tgctcgatgg ttttgacacc gcggctatcg gatttatcgc
    20281 ccctgatatt cgcactcact ggcaactcac cgcaggcgat ctcgccccgc tttttggcgc
    20341 gggtctgcta ggattaaccg caggcgcact gctttgtggc ccgttgtccg atcgctttgg
    20401 tcgcaagcgg gtgattgagc tgtgcgtctt tttgtttggc gcgttaagcc tcgcctcggc
    20461 tttctcaccg gatctgcaaa cgctggtctt cctgcgcttt ctcaccggcc tgggtctggg
    20521 cggcgcgatg ccgaacacca tcaccatgac ctcggagtat ctgcctgccc gccgccgcgg
    20581 cgcgctggtg acgctgatgt tttgcggctt tacgctgggt tcggcgtttg gcgggattgt
    20641 cagtgcgcag ctggtgccgg tgattggctg gcacggcatt ctggtgttgg ggggcgtgtt
    20701 accgctgatg ctgtttgtcg cactgctggt agttctgccg gaatcgccac gctggcaggt
    20761 gcgccgccag ctaccgcagg cggtgattgc gaaaaccgtc agcgccatca cccgcgagcg
    20821 ttatgtcgat acccactttt atctcattga atcagcctcc gttacaaaag gcagcattcg
    20881 tcaactgttt atgggacgtc agttacccat cacgttgatg ctgtgggtag tgttctttat
    20941 gagcctgctg attatttacc tgctctcaag ctggatgccg acgctactga accatcgcgg
    21001 catcgacctg caacacgcgt cctgggtcac cgccgcgttc cagattggtg gtacgttagg
    21061 cgcgctggcg ctgggtgtgc tgatggataa gttcaatcct ttccgcgtgc tggcgctgag
    21121 ctatgccata ggcgctatat gcatcgtgat gattggctta agccaggatg gattatggtt
    21181 gatggcgctg gcgattttcg gcactggcat tggcattagc ggatcgcagg tggggctgaa
    21241 tgcgctgacc gcaacacttt atccaacaca aagtcgcgcc acgggcgtga gctggtcaaa
    21301 tgctatcggc cgctgcggcg cgattgttgg ttcgctgtct ggcggcgtga tgatggcgat
    21361 gaatttctct ttcgacaccc tgtttttcat tatcgctgtg cctgcggcga tcagtgcggt
    21421 gatgttaacc ctgctgatta ccgtggttcg ccagtcgacc tctgtccctg actcactgcc
    21481 tcgcgcaggc gttgtgaacg aataaggagc atcccaatga ctgataacaa tcaaaatagc
    21541 cgcgagcaat tttatcagca tatttccggg caaaacctga ccccgctgtg ggagtcactg
    21601 caccatctgg tgccgaaaac gcccaacgct aactgtgcgc cagcgtactg gaattatcag
    21661 gagatccgcc cgctgctact ggagagcggc ggcctgattg gtgcgaaaga ggccgtgcgc
    21721 cgcgtgctgg tgctggaaaa cccggcgctg cgcgggcagt cttccattac cgcgacttta
    21781 tatgccggtt tgcaactgat catgccgggc gaagtggcac cgagtcatcg tcataatcag
    21841 tcggcgctgc gttttattgt tgaaggcaaa ggggcattta ccgccgttga tggcgaacgc
    21901 acgccaatga atgaaggcga ttttatcctg accccgcagt ggcgctggca cgatcacggt
    21961 aaccctggcg acgaaccggt tatctggctc gatgggctgg atctgccatt agtgaatatt
    22021 ctgggctgcg gttttgccga agattatccg gaagagcaac aaccggtaac gcgtaaagag
    22081 ggagattatc tgccgcgtta cgctgccaat atgttgccgc tgcggcatca gacggggaac
    22141 tcctcgccga tctttaacta tcgttatgac cgcagccgcg aagtgctgca cgatttaacc
    22201 cgactgggcg atgccgacga gtgggatggc tacaaaatgc gctacgtcaa cccggtcacc
    22261 ggcggctatc cgatgccgtc gatgggcgct ttcctgcaac tgctgccgaa agggtttgcc
    22321 tcacgcgttg cgcgtaccac tgacagcacc atctaccatg tggtggaagg tagcgggcag
    22381 gtcatcatcg gcaatgaaac cttcagcttt agtgcaaaag atatcttcgt ggtgccgacc
    22441 tggcatggcg tgtcgttcca gaccacgcaa gattccgttt tatttagttt ttcggacaga
    22501 ccggtacaag aagccctggg gctgttccgc gaagcgcgtt attaatttag gagagaaaaa
    22561 atgactcaat acgtattcga accgcaagcg cccgttaccg taccggttgt tggcagtgat
    22621 gaacaattcc ccgtgcgccg cgtttactgc gtcggacgta actatgccgc tcacgcccgc
    22681 gaaatgggct ttgatccgga tcgtgaaccg ccattcttct tctgcaaacc ggcggatgcc
    22741 gttgttccgg ttgccgctgg tgaaacgctg gcgctgccgt atccggcaca gaccgataac
    22801 taccattacg aaattgaact ggtggtggcg attggtaaaa aaggcagcga tatcccgctg
    22861 gaacacgccc atgaatatgt ctggggttat gccacgggtc ttgatatgac tcggcgtgac
    22921 cgtcaaatgg aaatgcgcca gatgggccgc ccgtgggaaa tcggtaaagc gtttgatctc
    22981 tcagcgccca tcgcgccgct gcacaaagcc cgcgatatcg gcggtattga taatgcgcca
    23041 atctggttac aggttaacgg cgaagatcat cagcgcagcg atattcgcca tctcatctgg
    23101 tcggtgaatg aaaccatcag ctatctgtca ggcttctttg agctgcaacc gggcgatctg
    23161 atcttcaccg gtacgccgga aggggttggc ccggtagtga aaggtgatgt gatcacaggc
    23221 aacgtcgagg gcttaacgcc tatcgcggtg aagattgtct gaggtgcatg atgaagctgt
    23281 acagtttttt taatagttca gcgtcgtacc gggtacgcat tgcgctggcg ctgaaaggca
    23341 ttaattacca gaccgaaggg gtgaatatcc gtattggtca gcaaaatgaa ctggcctatc
    23401 ggcggatgaa tccggtcggg ctggtgccga cgctgctgac tgacgagggc gaatcactcg
    23461 gacaatcgct ggcgattatt gactggctgg agaggcacta cccgcaagtt ccgctggtgc
    23521 cgcaggaaga gccggcccgc aacaaggtgc tggaaattgt ctatgccatc gcttgtgata
    23581 ttcatccatt gaataacttg cgcgtgctgc gctacctgac cgaagaactg aacgtcagtg
    23641 aagaagaaaa aaaacgttgg tatgcgcact ggatccagca agggttaagc gcggtggaac
    23701 aactgttgcg ccagaaccag tcggggcaat tttgtgtggg cgagacgccg acgctggcag
    23761 attgctgcct tgtcccgcag tgggcgaacg cgctacgcat gaactgcgat ttgagcgggt
    23821 atccacgctg caaagcggtg tacgacgcct gcacacagct gccagcgttc attgccgcag
    23881 cgccagaaaa tcaacaagat aaaatttcag cctgaccagg agaactataa tgacgaaagt
    23941 gacacgcgca gtaattgtgg gaggcgggat cggcggtgcg gcaactgcgc tgtcactggc
    24001 ccgcctgggg atcaaagtgg tgctgctgga aaaggcgcat gagatcggtg agattggcgc
    24061 gggcatccag ttggggccaa atgcgttttc ggcgctggac agcctcggcg tcggcgaagt
    24121 tgcccgccag cgcgcggtgt ttaccgatca cattaccatg atggatgcgg tgaacgccga
    24181 agaagtggtg tgcattgaaa ccgggcaggc ttttcgtgac catttcggcg gcccttacgc
    24241 ggtgatccac cgggttgata ttcacgcctc ggtgtgggaa gcggtactga cgcatccgga
    24301 cgtggaatat cacacctcca ccaacgtcgt ggatattcgc gagacggaag acgatgtcac
    24361 cgtgtttgac gatcgcggca atagctggac cggcgatatt ctgattggct gtgacggcgt
    24421 gaagtcagtg gtgcgccaga gcttattggg cgatgcgccg cgtgtcaccg gacatgttgt
    24481 ctaccgtgcg gttatcgatc gtgccgatat gccggaagat ttgcgcatta acgcaccggt
    24541 actgtgggcg gggccgcatt gtcacttggt gcattaccct ttgcgcggcg gcaagcagta
    24601 taacctggtg gtaacattcc acagccgcca gcaggaagag tggggcgtga aggatggtag
    24661 taaagaggaa gtgctctctt actttgaagg gattcacccc cgtccgcgcc agatgttgga
    24721 taaaccgact tcatggcgac gctggtcaac cgctgaccgt gaaccggtgg cgaagtgggg
    24781 aacgaagcgc ataacgcttg tgggcgattc ggcccatccg gtggcgcaat atatggcgca
    24841 gggagcctgt atggcgctgg aagatgcagt aacgctgggt aaggcgctgg cgcagtgtga
    24901 gggtgatgcg gcgcaggcct ttgcgctgta tgagtcggta cgaattccgc gcaccgcgcg
    24961 catcgtctgg tcgacccgtg aaatggggcg ggtttaccac gccgcagggg tagaacgcca
    25021 ggtacgtaat ctgctgtgga aagggaaatc gcaggcagaa ttttatcgcg gcatggagtg
    25081 gttgtacggc tggaaagaag ataactgttt gcaaccacgc tgagcacttc ttcacccaat
    25141 caggcgcacg ggcggtcccc tcgccccttc gggagagggt tagggtgagg gtaaaaatct
    25201 tgcccgaatt aatcagtaag tcagtttgtc tgcaaacaca ggaggcgcta acatagcgcc
    25261 tcattttttt gcgggtgatg atatgcgtgt gttactggca ccgatggagg gtgtgcttga
    25321 ctctctggtg cgtgaattgc tgaccgaagt taacgactac gatctgtgca tcaccgagtt
    25381 tgtccgcgtg gtggatcaac tgctgccggt aaaagtcttt catcgcattt gccctgagtt
    25441 acaaaacgcc agccggacac catccggtac gctggtgcgc gttcagttgt taggtcagtt
    25501 cccacaatgg ctggcagaga acgccgcccg tgcggtggaa ttaggttcct ggggcgtgga
    25561 tctcaattgc ggctgcccgt cgaaaacggt caacggtagc ggtggtgggg cgacgttact
    25621 caaagatcct gaactcatct accagggtgc aaaagcgatg cgtgaagctg taccggcgca
    25681 tttgcccgtc agcgtgaaag tgcgtctggg ctgggacagc ggtgagaaga aatttgaaat
    25741 cgccgatgcg gttcaacagg ctggtgctac ggagctggtg gtgcatggac ggacaaaaga
    25801 gcagggttac cgcgcggagc atattgactg gcaggcgatt ggtgagattc gccagcggct
    25861 gaatattccg gtgattgcca acggtgaaat ctgggactgg cagagcgcgc aacaatgcat
    25921 ggcgatcagc ggctgcgacg cagtgatgat tggtcgcggg gcgctcaata ttcccaacct
    25981 gagccgggtg gtaaaatata acgaaccgcg aatgccgtgg ccggaggtgg ttgctttgct
    26041 gcaaaaatat acccgtctgg aaaagcaggg cgataccggg ttatatcacg tagcgcggat
    26101 taaacagtgg ttgagttatt tgcgtaaaga atacgatgaa gcaacggaat tatttcagca
    26161 tgttcgggtg ttgaataatt cccatgatat tgcaagggct attcaggcaa ttgatatcga
    26221 taagctgcga taatagtgga atcgcagttt atcagagaca atgaatcgct tattcagctt
    26281 catcatcttg ttgcgctcct ttcatgaact aagccacata ttgccactgg cgcaaggagc
    26341 gcgcaggggg agggcaatcg cctcctcccc ctgcaccccc gggctctggc gaacaaaatc
    26401 gccgctgcgc ggtaccctca gcttatccct tgcggctacc gggtcgggcg cgaggtaaca
    26461 tccctgtaaa acgcgccctc agcccacatc catgtgggct gccccggcct tcagggaacg
    26521 cctcggcgat tttgacgcca ccaacaccgg tgcggtttgt aattaaagag caagaatatc
    26581 tttaaatgaa aatgaatacc ctaccgatcc ttatgcataa catttccttt taattgcaac
    26641 tttactcgtc cttccgcttt cagtagtgtg tcatcgcgca tttcgacttc tttctttgca
    26701 agggctgggt tttcatgttt tctgggttgt taatcattct ggttcccctg attgtgggtt
    26761 acctcattcc gcttcgccaa caagctgcgt taaaagttat taatcagcta ctaagctgga
    26821 tggtttacct tattctcttt tttatgggta tcagtctggc gtttctcgat aacctcgcca
    26881 gtaacctgtt ggcgattctg cattattctg ccgtcagtat taccgttatt ttactgtgta
    26941 atattgccgc ccttatgtgg ctggagcgcg gtctgccgtg gcgcaaccac catcagcaag
    27001 aaaaactccc gtcgcgtatt gcgatggcgc ttgagtcgct aaaactgtgc ggcgtagtag
    27061 tgattggttt tgccattggc ctgagtggac tggctttctt acaacacgcg accgaagcca
    27121 gtgaatacac cttaattctg ctacttttcc tcgtcggtat tcagttgcgc aataatggca
    27181 tgaccttaaa gcagattgtc cttaatcgcc ggggaatgat tgtcgccgtg gtggtggttg
    27241 ccagttcatt aattggtggt ttaattaacg cctttattct tgatctaccc atcaataccg
    27301 cgctggcaat ggcctctggt ttcggctggt attctctttc cggtatttta ttgaccgaat
    27361 cttttggtcc ggtaatcggg agcgcggcgt tttttaatga tctggcccgt gagctgattg
    27421 caattatgtt gatccctggg ctgattcgcc gcagccgctc taccgcactg ggcttatgcg
    27481 gtgcgacgtc gatggatttc accctgcccg ttcttcaacg tactggcggg ctggatatgg
    27541 tcccggcggc aattgttcac ggttttattc ttagcctgtt agtgccgatc ctcatcgcct
    27601 ttttctctgc gtaatacctc tctggcggta gatccctgcc gccaaaattg cgctaaatca
    27661 atctccctta aagttgcatg aaaaatccct tttatccccg cgttaagcgt cttaacctta
    27721 aagatgtata ttaaatataa ctttaaaagg tgtgatcatg ttttgtgtgc aatgtgaaca
    27781 aactatccgt actccggcag gaaacggctg ctcatacgcg caggggatgt gtggtaaaac
    27841 ggcggaaacc tctgaccttc aggatttact catcgcggcg ctgcaagggc tttcggcctg
    27901 ggcggtaaaa gcgcgtgaat acggcatcat caaccacgat gtagacagct ttgcgccacg
    27961 cgcatttttc tcaaccctga ccaacgttaa cttcgattct ccgcgtattg tcggctacgc
    28021 tcgtgaagcg attgccttgc gcgaggcgct gaaagcacaa tgcctggctg tagatgccaa
    28081 cgcccgcgtc gataacccga tggctgacct gcaactggtg agcgacgatc tcggcgaact
    28141 gcaacgtcag gcagcagaat ttacccctaa caaagataaa gcggcgattg gcgaaaacat
    28201 tctcggcctg cgtctgctgt gcctgtatgg cctgaaaggt gcggcggcct atatggaaca
    28261 cgcacacgtt ctcggtcaat acgacaatga tatttatgcc cagtaccata aaatcatggc
    28321 gtggctgggg acctggcctg ccgatatgaa cgcgcttctt gagtgttcaa tggaaatcgg
    28381 ccagatgaac ttcaaagtga tgagcattct ggatgcaggc gaaaccggta aatacggtca
    28441 cccgacgcca acccaggtca acgtcaaagc gacggcgggt aaatgcattc tgatttccgg
    28501 tcacgatctc aaagatctct acaaccttct ggaacagacc gaaggcacgg gtgttaatgt
    28561 ctacacccac ggcgaaatgc tgcctgcgca tggctacccg gagctgcgta aattcaagca
    28621 tctggtcggt aactacggca gcggctggca gaatcagcaa gtggagttcg ctcgtttccc
    28681 tggccccatc gtgatgacct cgaactgcat catcgaccca accgtaggcg cttatgacga
    28741 tcgtatctgg acccgcagca tcgttggctg gccgggtgtg cgtcatctgg atggcgaaga
    28801 tttctctgcg gtcattgccc aggcgcaaca aatggcgggc ttcccgtaca gcgaaattcc
    28861 gcacctgatc accgtgggct ttggccgcca gacgctgctt ggcgcagcgg atacgctgat
    28921 tgatctggtg agccgtgaaa aactgcgtca tatcttcctg cttggtggct gtgacggcgc
    28981 acgcggcgag cgccactact tcaccgattt cgccaccagc gtgccggatg actgcctgat
    29041 cctgaccctc gcctgtggta aatatcgctt taacaaactt gagtttggcg atatcgaagg
    29101 tctgccgcgt ctggtagatg ccggtcagtg taacgatgct tactcagcga ttattctggc
    29161 ggttacgctg gcagagaaac tgggctgcgg tgtcaacgat ctgccgctgt cgctggtgct
    29221 ctcctggttt gaacagaaag cgatcgtcat tctgctgacg ctgctttctc tgggcgtgaa
    29281 aaatatcgtc accggcccga ctgcacctgg tttcctgaca ccagatctgc tggcggtgct
    29341 gaacgagaaa ttcggcctgc gttctatcac cactgttgaa gaagacatga agcaactgtt
    29401 gagcgcgtaa ggagtttgtc gatgacgatg ccaacgaatc aatgcccgtg gcggatgcag
    29461 gttcatcaca ttaagcaaga aacgccggat gtgtggacga tttccctgat ttgccacgat
    29521 tactacccat accgcgccgg gcaatatgca ctggtcagcg tgcgtaactc agcggaaacg
    29581 ctgcgtgctt acaccatttc ctccacgcca ggcgtgagtg aatatatcac cctgaccgtg
    29641 cggcggattg atgacggtgt cggctcccag tggctgaccc gcgatgtaaa acgcggtgat
    29701 tatctctggc tttcggacgc gatgggggaa tttacctgcg acgataaagc agaagataaa
    29761 ttcctgttgc tggctgcagg ctgcggcgtc acgccgatta tgtcgatgcg tcgctggctg
    29821 gcgaagaatc gtccacaggc cgatgtacag gtgatctaca acgtgcgtac gccgcaggat
    29881 gttattttcg ccgatgagtg gcgtaactat ccggtaacgc tggtggcgga aaataacgtt
    29941 accgaaggct ttatcactgg tcgtctcact cgcgaactcc tgactcgcgt tcctgactta
    30001 gcttcacgta ccgtgatgac ttgtggtcca gcaccgtata tggattgggt agagcaggaa
    30061 gtgaaagcgc tcggcgtgac gcgtttcttt aaagagaaat tcttcacccc agtagcggaa
    30121 gcggcgacca gcggtctgaa attcaccaaa ctgcaaccgg cacgagaatt ttacgccccg
    30181 gttggcacta cgctactgga ggcgctggaa agcaataacg ttccggttgt cgctgcctgc
    30241 cgtgcgggtg tttgcggctg ctgtaagaca aaagtggttt ccggtgaata tacggtgagc
    30301 agcacaatga cgctgaccga cgccgaaatc gctgaaggtt acgtactggc ctgctcctgc
    30361 catccgcagg gggatttggt tctcgcataa tcgccttatg cccgatgata ttcatttcat
    30421 cgggctattt aaccgttagt gcctcctttt tctcccatcc cttccccctc cgtcagatga
    30481 actaaacttg ttaccgttat cacattcagg agatggagaa ccatgaaaca aacggttgca
    30541 gcttatatcg ccaaaacact cgaatcggca ggcgtgaaac gcatctgggg agtcacgggc
    30601 gactctctga acggtcttag cgacagtctt aatcgcatgg gcaccatcga gtggatgtcc
    30661 acccgccatg aagaagtggc ggcgtttgcc gctggcgctg aagcacaact ttcaggagaa
    30721 ctggcggtct gtgccggatc gtgcggcccc ggcaacctgc acttaatcaa cggcctgttc
    30781 gattgccacc gcaatcacgt tccggtactg gcgattgccg ctcatattcc ctccagcgaa
    30841 ataggcagcg gctatttcca ggaaacccac ccacaagagc tattccgcga atgtagtcac
    30901 tattgcgagc tggtttccag cccggagcag atcccacaag tgctggcaat tgctatgcgc
    30961 aaagcggtgc ttaaccgtgg cgtttccgtt gtcgtgttac cgggtgacgt ggcgttaaaa
    31021 cctgcgccag aaggggcaac cacgcactgg tatcatgcgc cacagccagt tgtgacgccg
    31081 gaagaagaag agttacgcaa actggcgcaa ctgctgcgtt attccagcaa tatcgctctg
    31141 atgtgtggca gcggctgcgc gggggcgcat aaagagttag ttgagtttgc cgggaaaatt
    31201 aaagcaccta ttgttcatgc cctacgcggt aaagagcatg tcgaatacga taatccgtat
    31261 gatgttggaa tgaccgggtt aatcggattc tcgtcaggtt tccataccat gatgaacgcc
    31321 gacacgttag tgctactcgg tactcagttc ccctaccgtg cgttctaccc gaccgatgcc
    31381 aaaattattc agattgatat caatccagcc agcatcggcg ctcacagcaa ggtggatatg
    31441 gcactggtcg gcgatatcaa atcaaccctg cgtgcattgc tgccactggt ggaagaaaaa
    31501 gccgatcgca aatttctgga taaagcgctg gaagattacc gcgatgcccg caaagggctg
    31561 gacgatttag ctaaaccgag cgagaaagcc attcacccgc aatatctggc gcagcaaatt
    31621 agtcattttg ccgccgatga cgccattttc acctgtgacg ttggtacgcc aacggtgtgg
    31681 gcagcgcgtt atctgaaaat gaacggcaag cgtcgcctgt taggttcgtt taaccacggt
    31741 tcgatggcta acgccatgcc gcaggcgctg ggcgcgcagg caaccgagcc ggaacgtcag
    31801 gtggtcgcca tgtgcggcga tggcggtttt agcatgttga tgggcgattt cctctcggta
    31861 gtgcagatga aactgccggt gaaaattatc gtctttaata acagcgtgct gggctttgtg
    31921 gcgatggaga tgaaagccgg aggctacctg acagacggta ctgagctgca cgacaccaac
    31981 tttgcccgca ttgccgaagc atgcggcatt acgggtatcc gtgtagaaaa agcgtctgaa
    32041 gttgatgaag ccctgcaacg cgccttctcc atcgacggtc cggtattggt ggatgtggtg
    32101 gtcgccaaag aagagttagc cattccaccg cagatcaaac tcgaacaggc caaaggtttc
    32161 agcctgtata tgctgcgcgc aatcatcagc ggacgcggcg atgaagtgat cgaactggcg
    32221 aaaacgaact ggctaaggta aaaagggtgg catttcccgt cataataagg acatgccatg
    32281 attgatttac gcagtgatac cgtaacccga ccgagccgcg ccatgctcga agcgatgatg
    32341 gccgccccgg ttggggacga cgtttacgga gacgacccta ccgttaatgc tctgcaggac
    32401 tatgcagcag agctttccgg taaagaagcc gccatttttc tgcctaccgg cactcaggcc
    32461 aacctggtcg ctctgctcag tcactgcgaa cgcggcgaag agtatattgt cggtcaggcc
    32521 gcgcataact atctgtttga agccggtggc gcagcggtgc tgggcagtat tcagccgcaa
    32581 cccatcgacg cggctgccga cggcacgcta ccgctgaata aagtggcgat gaaaatcaaa
    32641 cccgacgata tccatttcgc ccgcaccaaa ttactcagtc tggaaaacac ccacaacggc
    32701 aaagtgttgc cgcgtgaata ccttaaagat gcatgggaat ttacccgcga gcgcaatctg
    32761 gcgctgcatg ttgacggtgc gcgcatcttt aatgccgtgg tggcttacgg ctgcgaactg
    32821 aaagagatca cgcaatattg tgattcgttc accatttgcc tgtcgaaagg tcttgggacg
    32881 ccagtcggtt cattactcgt cggtaatcgt gattacatta aacgtgccat tcgctggcgg
    32941 aaaatgacag gtggcgggat gcgccagtcc ggcattctgg ctgccgccgg gatatatgcc
    33001 ctgaaaaata atgttgcgcg cttgcaggaa gaccacgaca acgctgcctg gatggcggag
    33061 cagctgcgtg aagcaggcgc ggatgtgatg cgtcaggaca ctaatatgct gtttgttcgc
    33121 gtcggggaag aaaatgctgc cgcgttaggc gaatacatga aagcgagaaa cgtgctgatt
    33181 aacgcctcgc cgattgtccg cctggtgacg catcttgacg tctcgcgcga acagctggcg
    33241 gaagtcgccg cccactggcg cgcattcctg gcgcgttaag gagagaaacg tgccgcaacg
    33301 cattttagtt ctcggtgcca gtggctacat tggtcagcat ctggtgcgca cactcagcca
    33361 gcaagggcat cagatcctgg cggcggcacg tcatgtcgac aggcttgcaa agctgcaact
    33421 ggcaaacgtc agttgccata aagtcgatct cagctggccg gataaccttc cggccctgtt
    33481 acaggatatc gataccgtct attttctggt gcacagcatg ggcgaaagcg gcgattttat
    33541 cgctcaggag cgccaggtgg ctctcaacgt ccgcgatgcg ctacgtgaag taccagttaa
    33601 gcaattaatc tttctcagtt cgttacaggc cccgccacat gagcagtcgg atcatctgcg
    33661 tgctcgtcag gctacggcgg atattcttcg tgaagcaggt gtcccggtga ccgaactgcg
    33721 ggccggaatt atcgttggcg caggttcagc agcgttcgaa gtcatgcgcg atatggtcta
    33781 caatctgcca gtgttaacgc cgccacgctg ggttcgttca cgcaccacgc ccatcgcgct
    33841 ggaaaacttg ctgcactatc tggtggcgct gttagatcat ccggccagcg aacaccgcat
    33901 cttcgaagcc gccggaccag aggtgctcag ttatcagcaa cagtttgaac attttatggc
    33961 ggtgagcggt aagcgccgct ggttgatccc catccccctc cccactcgct ggatttcggt
    34021 gtggtttctt aatgtgatta cttccgtacc gcccaccacc gccagggcgt tgattcaggg
    34081 gctgaaacac gatctgctgg cggatgacac cgcgctacgt gcactcatcc cacaacggct
    34141 gatcgctttc gatgacgcgg tacgtagcac gttgaaagag gaagaaaaac tggtcaactc
    34201 cagcgactgg ggctacgacg ctcaggcctt tgcccgctgg cgacctgagt acggttattt
    34261 cgccaaacag gcggggttta ccgttaaaac gtccgccagc cttgcggctt tatggcaggt
    34321 agtgaaccaa atcggcggta aagagcgtta tttctttggc aatattttgt ggcagacacg
    34381 ggcgttgatg gaccgtgcga tcggtcataa attagcgaaa ggccgtccgg agcgcgaata
    34441 tttgcaaact ggcgatgcgg tggatagctg gaaagtcatt gtcgttgaac cgaaaaagca
    34501 actttcgttg ttatttggca tgaaagcacc ggggctggga cgactgtgtt ttaccctgga
    34561 agataaaggc gactatcgta ctatcgatgt ccgcgctttc tggcatccgc acggtatgcc
    34621 ggggctgttt tactggttat tgatgatccc cgcgcatctg tttatttttc gcggaatggc
    34681 aaaacaaatc gccagactgg cagaacaaag cacagattaa tcgacaaaac ctgcgttatt
    34741 ctttcatgtt tcccattgca tcatggggga aatcacggaa gaatgcgcac gtcattttct
    34801 caacaacagt ggattgatat gaaggtactg gttaccggcg ccaccagcgg cttaggtcga
    34861 aacgcggtag agtttttatg ccagaaaggc atcagcgtgc gagcgaccgg tcgcaacgag
    34921 gcaatgggta aattgctgga aaaaatgggc gcagagtttg ttccggcgga tctgaccgag
    34981 ctggtctcat cacaagctaa agtgatgctc gcgggcattg atacgctgtg gcactgctcc
    35041 agctttacct ctccctgggg gacacaacag gctttcgatc tggctaacgt tcgcgccact
    35101 cgccgcctgg gtgaatgggc tgtcgcctgg ggtgtgcgta actttattca tatctcttct
    35161 ccctccctgt acttcgatta tcaccaccat cgcgatatta aagaagattt tcgccctcac
    35221 cgcttcgcca acgagtttgc ccgcagcaaa gcagccagcg aagaagtgat caatatgctt
    35281 tcgcaggcga atccacaaac gcgctttact attctgcgcc cacaaagtct gttcggaccg
    35341 cacgataaag tctttatccc ccgtcttgcg catatgatgc accactacgg cagcattctg
    35401 ttaccgcacg gcggaagtgc gctggtcgat atgacctact atgaaaatgc agtgcacgcc
    35461 atgtggctgg caagccagga agcctgcgat aagctacctt ccgggcgtgt gtacaacatt
    35521 accaacggcg agcatcgcac actgcgcagc atcgtgcaga agctgatcga cgagttgaat
    35581 attgactgtc gtattcgttc cgtcccctac ccgatgctgg atatgatcgc ccgcagcatg
    35641 gagcgtttag gccgcaagtc agcaaaagag ccgccgctga cccactacgg cgtctcgaag
    35701 cttaattttg actttacgct ggatattacg cgtgcacagg aagaattagg ttatcagccg
    35761 gtcatcaccc tggatgaagg tatcgagaaa actgccgcct ggctacgcga ccacggaaaa
    35821 ctgccgcgct aatcctgccc gtatttctcc agcaacgctt cggcaattgc ctgagtttct
    35881 gcatccgctt cgccgttgta taacgtcggg cggaaatgca tctggaacgc cataatcacg
    35941 cgccgctgct cgcgcggtct catatcaggt ttaacgtcat aaccgtagcg cgccaaaagc
    36001 tccagcaatg acgcggtatc taccggtgtg tgcggcgcgc gcccggcaag gtaaaagttc
    36061 acccgctgcg catccggcca ggcaccaatc ccctgctgcg ccagttgctg ccagggaaat
    36121 aatggccccg gatcgtcttt gcgctgcggt gcgatatccg catgtgccac tacgttttcc
    36181 ggcttgatgt gataacgggc aataatatct ttcgccagtg gaataagcgc ctgaatctgt
    36241 gccggttcaa acggggcaaa atatttcact ccggccgatt tttgccagcc acggttttcc
    36301 agctcaatgc caatagaggt gtcgttaagg cgcgttgccc cgcgccaggt gctaatcccc
    36361 gcatgccagg ccagttcttg ttccggcacc agttgccaga tgcgcggttt accgttgtat
    36421 cgcggtggta ccgcagggac cagataatgc gagctgacct gcttatcggt cagtgtcgcc
    36481 agcgagctat caaaatcatc tgcggtgtag tggatcacca gcactttaat gcgcggatac
    36541 gccgcctgcg cctggcgtcg ggtatcaagc tggtatccct ctttctcgac aatgcctttt
    36601 tcgcctgcac accctgccag taacagagcg gcagcgacca gccagaagac tcttctcatc
    36661 gacgcgtttt caccgccgta ccgctgacgc taaccatcag catactgccg ttttgcccga
    36721 ccgtttcgta gtcgatatca ataccaacta cggcatcggc ccccagcgcc cgcgcctggg
    36781 agcctaattc ctcaaaggcg atctcccgtg ctttacgcag ttctttttca tacgcaccgg
    36841 aacgtccgcc aacgatatcg cggataccgg caaagaaatc acggaaaata ttggcaccta
    36901 aaatcgcttc gccggtcacc acaccgcaat attcaacgat ggtctggcct tccagagttg
    36961 gagttgtgga aaattgcatg atcgatctcc ttgtttgtta agccttcgat ctctaaagca
    37021 ttatcggact gatatgccat gttggaaaga aggttttgat cagtttagga aatcatctca
    37081 ttgtgtcaaa aacagctaca taagcctgta caattatctg tttcgtaacc ggatattggt
    37141 ttcattatgg agcaccgctt acctgcccta ttatgttgtg ccggcaacat taatgagtgg
    37201 gaagagtcaa cttatattgt aaatgattcc taaagtcttc cccatcttgc gcctgaggta
    37261 tataccgctg gattgctttt tggtccgccg ccgcaaaaca agaaagtaat aagatctcaa
    37321 tgcttttgtc attaatataa gcgaagatca ttcttgcaaa ttcgtatata aacgcccgat
    37381 cctgagcgct taatagttta atacaatatt tatttttaag agaataaagt atttcaagaa
    37441 aagcatccgc agcaaccttc acgtta
//
LOCUS       DAZFKF010000042        37069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFKF010000042 DAZFKF010000000
VERSION     DAZFKF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.042"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(33..164)
                     /locus_tag="ACHKA1_002526"
     ncRNA           complement(33..164)
                     /ncRNA_class="other"
                     /locus_tag="ACHKA1_002526"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(208..292)
                     /locus_tag="ACHKA1_002527"
     tRNA            complement(208..292)
                     /locus_tag="ACHKA1_002527"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(256..258),aa:Tyr,seq:gta)
     gene            complement(452..1294)
                     /gene="purU"
                     /locus_tag="ACHKA1_002528"
     CDS             complement(452..1294)
                     /gene="purU"
                     /locus_tag="ACHKA1_002528"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO5680165.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1344..1802)
                     /gene="ychJ"
                     /locus_tag="ACHKA1_002529"
     CDS             complement(1344..1802)
                     /gene="ychJ"
                     /locus_tag="ACHKA1_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO5680166.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAVLRAELIAGFAHTEWLGLTVFEHCWQDVDNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            1915..2820
                     /gene="rssA"
                     /locus_tag="ACHKA1_002530"
     CDS             1915..2820
                     /gene="rssA"
                     /locus_tag="ACHKA1_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO5680167.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            2912..3925
                     /gene="rssB"
                     /locus_tag="ACHKA1_002531"
     CDS             2912..3925
                     /gene="rssB"
                     /locus_tag="ACHKA1_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO5680168.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            4127..5035
                     /gene="galU"
                     /locus_tag="ACHKA1_002532"
     CDS             4127..5035
                     /gene="galU"
                     /locus_tag="ACHKA1_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO5680169.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(5179..5592)
                     /gene="hns"
                     /locus_tag="ACHKA1_002533"
     CDS             complement(5179..5592)
                     /gene="hns"
                     /locus_tag="ACHKA1_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO5680170.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            6196..6813
                     /gene="tdk"
                     /locus_tag="ACHKA1_002534"
     CDS             6196..6813
                     /gene="tdk"
                     /locus_tag="ACHKA1_002534"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO5680171.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(7115..9790)
                     /gene="adhE"
                     /locus_tag="ACHKA1_002535"
                     /gene_synonym="adhC"
     CDS             complement(7115..9790)
                     /gene="adhE"
                     /locus_tag="ACHKA1_002535"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO5680172.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            10183..10236
                     /gene="ychE"
                     /locus_tag="ACHKA1_002536"
     CDS             10183..10236
                     /gene="ychE"
                     /locus_tag="ACHKA1_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO5680173.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            10267..10914
                     /gene="ychE"
                     /locus_tag="ACHKA1_002537"
     CDS             10267..10914
                     /gene="ychE"
                     /locus_tag="ACHKA1_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO5680174.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSFQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(11072..11368)
                     /locus_tag="ACHKA1_002538"
     CDS             complement(11072..11368)
                     /locus_tag="ACHKA1_002538"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680175.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNHLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            11652..13283
                     /gene="oppA"
                     /locus_tag="ACHKA1_002539"
     CDS             11652..13283
                     /gene="oppA"
                     /locus_tag="ACHKA1_002539"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO5680176.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            13369..14289
                     /gene="oppB"
                     /locus_tag="ACHKA1_002540"
     CDS             13369..14289
                     /gene="oppB"
                     /locus_tag="ACHKA1_002540"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO5680177.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            14304..15212
                     /gene="oppC"
                     /locus_tag="ACHKA1_002541"
     CDS             14304..15212
                     /gene="oppC"
                     /locus_tag="ACHKA1_002541"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO5680178.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            15224..16237
                     /gene="oppD"
                     /locus_tag="ACHKA1_002542"
     CDS             15224..16237
                     /gene="oppD"
                     /locus_tag="ACHKA1_002542"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO5680179.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            16234..17238
                     /gene="oppF"
                     /locus_tag="ACHKA1_002543"
     CDS             16234..17238
                     /gene="oppF"
                     /locus_tag="ACHKA1_002543"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO5680180.1"
                     /translation="MNAVTEGRKVLLEIDDLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(17291..17620)
                     /gene="yciU"
                     /locus_tag="ACHKA1_002544"
     CDS             complement(17291..17620)
                     /gene="yciU"
                     /locus_tag="ACHKA1_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO5680181.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(17655..19115)
                     /gene="cls"
                     /locus_tag="ACHKA1_002545"
     CDS             complement(17655..19115)
                     /gene="cls"
                     /locus_tag="ACHKA1_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO5680182.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            19258..19431
                     /gene="yciY"
                     /locus_tag="ACHKA1_002546"
     CDS             19258..19431
                     /gene="yciY"
                     /locus_tag="ACHKA1_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO5680183.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(19485..20738)
                     /gene="kch"
                     /locus_tag="ACHKA1_002547"
     CDS             complement(19485..20738)
                     /gene="kch"
                     /locus_tag="ACHKA1_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO5680184.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(21039..21335)
                     /gene="yciI"
                     /locus_tag="ACHKA1_002548"
     CDS             complement(21039..21335)
                     /gene="yciI"
                     /locus_tag="ACHKA1_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO5680185.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            21559..22278
                     /gene="tonB"
                     /locus_tag="ACHKA1_002549"
     CDS             21559..22278
                     /gene="tonB"
                     /locus_tag="ACHKA1_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO5680186.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTASARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(22318..22716)
                     /gene="yciA"
                     /locus_tag="ACHKA1_002550"
     CDS             complement(22318..22716)
                     /gene="yciA"
                     /locus_tag="ACHKA1_002550"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO5680187.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(22821..23360)
                     /gene="yciB"
                     /locus_tag="ACHKA1_002551"
     CDS             complement(22821..23360)
                     /gene="yciB"
                     /locus_tag="ACHKA1_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO5680188.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(23390..24133)
                     /gene="yciC"
                     /locus_tag="ACHKA1_002552"
     CDS             complement(23390..24133)
                     /gene="yciC"
                     /locus_tag="ACHKA1_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO5680189.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(24161..24250)
                     /locus_tag="ACHKA1_002553"
                     /pseudo
     CDS             complement(24161..24250)
                     /locus_tag="ACHKA1_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            24490..25128
                     /gene="ompW"
                     /locus_tag="ACHKA1_002554"
     CDS             24490..25128
                     /gene="ompW"
                     /locus_tag="ACHKA1_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO5680190.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSELSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(25188..25694)
                     /gene="yciE"
                     /locus_tag="ACHKA1_002555"
     CDS             complement(25188..25694)
                     /gene="yciE"
                     /locus_tag="ACHKA1_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO5680191.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(25740..26240)
                     /gene="yciF"
                     /locus_tag="ACHKA1_002556"
     CDS             complement(25740..26240)
                     /gene="yciF"
                     /locus_tag="ACHKA1_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO5680192.1"
                     /translation="MNMKTMEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(26326..26505)
                     /gene="yciG"
                     /locus_tag="ACHKA1_002557"
     CDS             complement(26326..26505)
                     /gene="yciG"
                     /locus_tag="ACHKA1_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO5680193.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQNGGNKSGKS"
     gene            complement(26886..27692)
                     /gene="trpA"
                     /locus_tag="ACHKA1_002558"
     CDS             complement(26886..27692)
                     /gene="trpA"
                     /locus_tag="ACHKA1_002558"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO5680194.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(27692..28885)
                     /gene="trpB"
                     /locus_tag="ACHKA1_002559"
     CDS             complement(27692..28885)
                     /gene="trpB"
                     /locus_tag="ACHKA1_002559"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO5680195.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTKTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(28897..30258)
                     /gene="trpCF"
                     /locus_tag="ACHKA1_002560"
     CDS             complement(28897..30258)
                     /gene="trpCF"
                     /locus_tag="ACHKA1_002560"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO5680196.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNIEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(30259..31854)
                     /gene="trpD"
                     /locus_tag="ACHKA1_002561"
     CDS             complement(30259..31854)
                     /gene="trpD"
                     /locus_tag="ACHKA1_002561"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO5680197.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(31854..33416)
                     /gene="trpE"
                     /locus_tag="ACHKA1_002562"
     CDS             complement(31854..33416)
                     /gene="trpE"
                     /locus_tag="ACHKA1_002562"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO5680198.1"
                     /translation="MQTQKPTLELLTCEGAYHDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LSFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(33438..33531)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(33508..33552)
                     /gene="trpL"
                     /locus_tag="ACHKA1_002563"
     CDS             complement(33508..33552)
                     /gene="trpL"
                     /locus_tag="ACHKA1_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO5680199.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            33690..34571
                     /gene="rnm"
                     /locus_tag="ACHKA1_002564"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             33690..34571
                     /gene="rnm"
                     /locus_tag="ACHKA1_002564"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO5680200.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDIIHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            34568..35188
                     /gene="yciO"
                     /locus_tag="ACHKA1_002565"
     CDS             34568..35188
                     /gene="yciO"
                     /locus_tag="ACHKA1_002565"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO5680201.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            35216..>37069
                     /gene="yciQ"
                     /locus_tag="ACHKA1_002566"
     CDS             35216..>37069
                     /gene="yciQ"
                     /locus_tag="ACHKA1_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFO5680202.1"
                     /translation="MAGKFRCILLLIVGLFFSSLSYAKNTEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLQFSNIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTLGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQSDKNQQTLIRV
                     NEGVTRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSSF
                     YRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFTR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAVSAN
                     LGREWVARITPSLTAAVSAPEIARSGVLFSLQMHLSLGANTSLLGRSYSGGG"
BASE COUNT         9420 a         8787 c         9249 g         9613 t
ORIGIN      
        1 aggtaattca aattttgagg taatgcttga ggtgctggtg ggggaaggat tattcgtcgc
       61 ttcgctcctc acccttcggg ccgttgcctg tggcaatgtt ctctcgcttt cgctcgagtc
      121 gaaccttagt cgaaggttct cacccttccc cgatgagtgc aaactttcac aatctcaccg
      181 aagttaccac atcgctgtgg tgaatcatgg tggtggggga aggattcgaa ccttcgaagt
      241 cgatgacggc agatttacag tctgctccct ttggccgctc gggaacccca ccacggggta
      301 atgcttttta ctggcctgct cccttatcgg gaagcggggc gcatcatatc aaatgacgcg
      361 ccgctgtaaa gtgttacgtt gagaaaaatg aactggttgc gtaattttta tccgtaacgg
      421 attaaaggta accagttatt tttgctggcg attaaagaat aatcgtccga ttaccgtaaa
      481 caaagacgcg ctgcgccagt actttgtaaa gcgcacgact taagacgttt ttctcgacgt
      541 cacgacctgc gcgcatcata tcttcagctg tgtaggtatg atcgacatga ataacgtcct
      601 gcatgatgat tgggccttcg tccagattgt cattcacata gtgagcggtt gcaccaataa
      661 tcttcacgcc acgttcatag gcctggtgat aaggacgtgc gccgataaac gctggcagga
      721 aggaatggtg aatattgatg atcttattcg ggaagcgtga cacaaattcc ggtgttaata
      781 cccgcatata cttcgccagc accacgtagt caggttgata agcatcaatg gcatccgcca
      841 tcttttgatc gtgctcgttg cggcttaacc cttcatggct taccagctca aacggtatat
      901 caaaacgctc aaccagagaa cgtaaagtat cgtggttacc aataactgcc gcgatttcga
      961 catccaggcc gccgtaattg gctttcatca acaaatcgcc aaggcaatgc gcttctttag
     1021 tgaccagaat cactatccgg cgacgaccgg caggattcag ctcacgcacg gagccttctg
     1081 gcaatgcgct atcgagatcc gccagcaggg tggaatcatt aaaaatccct tccagttccg
     1141 tgcgcataaa aaagcgcccg gtacggtgat caacaaattc attgttctgt acgatattta
     1201 actcgtgctt gtagcaaata ttggtaatac gtgcgatcag acctttttgg tccggacaaa
     1261 tagtacgcag aactttacgt tggagtgaat gcattgctgg aaaaaccttg ttgagagtgt
     1321 ttgctaaacc gtaccgtcaa ccattattgg ccgcagcact ttttaaattt tttacctgaa
     1381 ccacaagggc agggatcgtt gcggccaaac tgcggacgtg taccgtcaat atagtaccat
     1441 tgaccgtttt ctttcaaaaa tcgcgaacgt tcaattattg cacctgtttt gccaccttcg
     1501 gtaaatcgag caacaaaact gacaaatccg atattgtcga cgtcctgcca acaatgttca
     1561 aatacggtca atcccaacca ttcagtatgc gcaaatccgg caatcaattc ggcacgtaac
     1621 accgctgccc cacaagatgg atgccaggtc tttattaaat aatctgcgtc ttgcatcaca
     1681 aaagcgcagt aacgcgaacg catgagatgt tctggatcag gtgcaacctt ttcaccagac
     1741 acataagggt ggcaacatag gctatactcg acagcactac cacagggaca aagctgagac
     1801 acaaataatc tccctggaaa caataacggc gtattaaccg cctgagtagc actatgttaa
     1861 ccgagcagta gcgatgtggc tacgattgca ttccagggga atcttgcggg aataatgaga
     1921 aagataaaaa tagggctggc gctgggatct ggcgcggcga gaggttggtc gcatattggc
     1981 gttattaatg cgctaaaaaa agtgggtatt gaaattgata tcgttgcagg atgttctatt
     2041 ggttcgctgg tgggcgcggc ctatgcatgc gatcgattat ctgcgctgga agattgggta
     2101 acctctttca gttattggga tgttttacgc ctgatggatc tctcctggca gcgcggtggg
     2161 ttactgcgcg gcgagcgtgt cttcaatcaa taccgcgaaa taatgccgga aacagagatc
     2221 gaaaattgtt cccgtcgctt cgcggctgtt gccaccaatt taagtactgg gcgtgaatta
     2281 tggtttactg aaggcgatct ccatcttgct attcgcgcat catgcagtat tccaggacta
     2341 atggcacccg ttgcgcataa cggctactgg ctggttgatg gggcagtcgt taacccaatt
     2401 cctatttcgc tcacgcgtgc attgggtgct gatattgtga tagcggtcga cctgcagcac
     2461 gatgctcatt tgatgcaaca agatttgctc tcctttaatg tcagtgaaga aaatagcgag
     2521 aatggtgatt ctctgccgtg gcatgcgcgt ctgaaagaaa ggttaggcag cataacgaca
     2581 cgtcgggcgg tgacagcacc aacggcaaca gagattatga ccacttctat ccaggtgctg
     2641 gagaaccgcc ttaaaaggaa ccgcatggca ggtgatccgc ccgatattct gattcaacct
     2701 gtttgcccgc aaatatctac gcttgatttc catcgcgcgc acgctgccat tgcggccggg
     2761 cagctggcag tggaaaagaa aatggacgaa cttttgccgt tggtacgcac caacatttga
     2821 ccagaatttt tatctacact taagttaatt ctgacaggcg caggtggcaa tagcatgcca
     2881 ctattgagta aagccagtca ggggagagaa catgacgcag ccattggtcg gaaaacagat
     2941 tctcattgtt gaagatgagc aggtatttcg ctcgcttctg gattcgtggt tttcctcatt
     3001 gggagcgaca acggtactgg cggctgatgg ggtggatgcc cttgagttgc tgggaggttt
     3061 cactccagac ctgatgatat gtgatatcgc gatgccacga atgaacgggc ttaaactgct
     3121 ggagcatata cgtaacagag gcgaccagac cccagttctg gtgatatctg ccactgaaaa
     3181 tatggcagat attgccaaag cgttacgtct gggcgttgaa gatgttttgc tgaaaccagt
     3241 taaagatctg aatcgcttgc gcgagatggt ttttgcctgt ctctatccca gcatgtttaa
     3301 ttcgcgcgtt gaggaagagg aaaggctttt tcgcgactgg gatgcaatgg ttgataaccc
     3361 tgccgcagcg gcgaaattat tacaggaact acaaccgccg gttcagcagg tgatttccca
     3421 ttgccgggtt aattaccgtc aattggttgc cgcggacaaa cccggcctgg tgcttgatat
     3481 tgccgcactt tcggaaaacg atctggcatt ttattgcctt gatgtaaccc gagctggaca
     3541 taatggcgta cttgctgcct tgttattacg cgcattgttt aacggattat tacaggaaca
     3601 gcttgcacac caaaatcaac ggttgccaga gttgggcgcg ttattgaagc aggtaaacca
     3661 tttactccgt caggccaatc tgccggggca gtttccgcta ttagttggct attatcatcg
     3721 cgaactgaaa aatctcattc tggtttctgc gggtctgaat gcgacgttaa ataccggcga
     3781 acaccaggtg caaatcagta atggtgttcc gttaggcact ttaggtaacg cttatttgaa
     3841 tcaattgagc cagcgatgcg atgcctggca atgccaaata tggggaaccg gtggtcgact
     3901 gcgcttgatg ttgtctgcag aatgagcaaa cgatgacgcg ggctaaattt gcattaccag
     3961 ctaatgtcgg ctggtggtac tatcgtcgcc attcgtataa gtaattgtct taattatgct
     4021 aactcgcatc cttttcagaa cttagcccct ccggggtgct gatatactgg gatgcgatac
     4081 agaaatatga acacgttcaa aacacgaaca gtccaggaga atttaaatgg ctgccattaa
     4141 tacgaaagtc aaaaaagccg ttatccccgt tgcgggatta ggaaccagga tgttgccggc
     4201 gacgaaagcc atcccgaaag agatgctgcc acttgtcgat aagccattaa ttcaatacgt
     4261 cgtgaatgaa tgtattgcgg ctggcattac tgaaattgtg ctggttacac actcatctaa
     4321 aaactctatt gaaaaccact ttgataccag ttttgaactg gaagcaatgc tggaaaaacg
     4381 tgtaaaacgt caactgcttg atgaagtgca gtctatttgt ccgccgcacg taactattat
     4441 gcaagttcgt caggggctgg cgaaaggcct gggacacgcg gtattgtgtg ctcacccggt
     4501 agtgggtgat gaacctgtgg ctgttatttt gcctgacgtt attctggatg aatatgaatc
     4561 cgatttgtca caggataacc tggcagagat gatccgccgc ttcgatgaaa cgggtcatag
     4621 ccagatcatg gttgaaccgg ttgctgatgt gaccgcatat ggcgttgtgg attgcaaagg
     4681 cgttgaatta gcgccgggtg aaagcgtacc gatggttggc gtggttgaaa aaccaaaagc
     4741 ggatgttgcg ccgtctaatc tcgctattgt gggtcgttac gtacttagtg cggatatttg
     4801 gccgttgctg gcaaaaaccc ctccgggagc tggtgatgaa attcagctca ccgacgcaat
     4861 tgatatgcta atcgaaaaag aaacggttga agcctatcat atgaaaggga agagccatga
     4921 ctgcggtaat aaattaggtt acatgcaggc cttcgttgaa tacggtattc gtcataacac
     4981 ccttggcacg gaatttaaag cctggcttga agaagagatg ggcattaaga agtaacatcc
     5041 gtatcggtgt tatccacgaa acggcgttga gcaatcgacg ccgttttttt atagcttatt
     5101 cttattaaat tgtcttaaac cggacaataa aaaatcccgc cgatggcggg attttaagca
     5161 agtgcaatct acaaaagatt attgcttgat caggaaatcg tcgagggatt taccttgctc
     5221 atccattgct tttttgatta cagctggagt acggccctgg ccagtccagg ttttagtttc
     5281 gccgttttcg tcaacgtagc tatattttgc cggacgctga gcacgtttag ctttggtgcc
     5341 agatttaacg gcggcaaggc tattcagcag ctcgttcggg tcaataccgt cagcgatcag
     5401 catttcgcga tattgctgca gtttacgagt gcgctcttca acttcagcag cagccgcgct
     5461 ttcttcttcg cgacgttcgt taacaacaac ttctaatttt tccagcattt cttccagcgt
     5521 ttcaagtgta cattctcttg cctgcgcacg aagagtacgg atgttgttca gaattttaag
     5581 tgcttcgctc attgtagtaa tctcaaactt atattggggt ggtttgttga ggtaataata
     5641 gagccttaaa ttcagttgtg caatagccag gaatgtaagt aattcaaaat tgttctttat
     5701 tttgtgccgc caataaatat cttttcataa aattagccag aaaagacgcg gcatatagcc
     5761 ctatttgtac cgatgatttc gcagcacgtg aggttaaaac ttcctgattc atgtcacatt
     5821 ttatggggag attatcgtaa gctgacgacc tttcagtctt ctgtattagt cgtgtttacg
     5881 agaattccct attaagcgaa tgatgaaaag tagaacagtc gcaataagag cagggactta
     5941 gtattgcact atctcctgga ggtcaacaga gggctattac ttgcgcaaca ggttaaagat
     6001 tgtgaatagt taccagcagt catttacccg cttataacaa gcgaggcagt tgtaatgata
     6061 gctcagaagg attatgcaag gcttcgtaag ggagaacgca tataccactt ctgtgcatac
     6121 tgttgagctg aaaaactgac gaattatgat aaactccagc caactttatt tcatatcatt
     6181 gagggcctgt ggctgatggc acagctatat ttctactatt ccgcaatgaa tgcgggtaag
     6241 tctacagcat tgttgcaatc ttcatacaat taccaggaac gcggcatgcg cactgtcgta
     6301 tatacggcag aaattgatga tcgctttggt gccgggaaag tcagttcgcg tataggtttg
     6361 tcatcgcctg caaaattatt taaccaaaat tcatcattat ttgatgagat tcgtgcggaa
     6421 catgaacagc aggcaattca ttgcgtactg gttgatgaat gccagttttt aaccagacaa
     6481 caagtatatg aattatcgga ggttgtcgat caactcgata tacccgtact ttgttatggt
     6541 ttacgtaccg attttcgagg tgaattattt attggcagcc aatacttact ggcatggtcc
     6601 gacaaactgg ttgaattaaa aaccatctgt ttttgtggcc gtaaggcaag catggtgctg
     6661 cgtcttgatc aagcaggcag accttataac gaaggtgagc aggtggttat tggtggtaat
     6721 gaacgatacg tttctgtatg ccgtaaacac tataaagagg cgttacaagt cggctcatta
     6781 acggctattc aggaaaggca tcgccacgat taataagaat ttctttactg acagggtgag
     6841 cagggcactt ttatcctgtc agttcgtttt acgcacttct tccgggctat acacccttct
     6901 cggcagtttt ttaacgccgc tatacgcctc acagggctct taagcaccga cgttgacttg
     6961 tgatctgtaa agtacaatat cgctgtgttt aggcgttata catcgtcgca aatatgatga
     7021 aggctaatgc tgtcggttta tggaaaagtt gctttgggta aacaaaaaaa cggccccaga
     7081 aggggccgtt tatgttgcca gacagcgcta ctgattaagc ggattttttc gcttttttct
     7141 cagctttagc cggagcggct tcttttttcg ctgcagtttc accttctaca taatcacgac
     7201 cgtagtaggt atccagcaga atctgtttca gctcggagat cagcgggtaa cgcgggttag
     7261 cgccggtgca ctggtcatcg aatgcatctt cagacagttt atccacgttc gccaggaagt
     7321 ctgcttcctg aacgccagct tcacggatag atttcggaat acccagttca gccttcagcg
     7381 tttccagcca tgccagcagt ttctcgatct tagcagcagt acggtcgccc ggtgcgctca
     7441 gacccaggtg gtcggcaatt tcagcataac gacggcgagc ctgcggacgg tcatactggc
     7501 tgaatgcagt ctgcttggtc gggttgtcgt tcgcattgta gcgaataacg ttacaaatca
     7561 gcagggcgtt tgccagaccg tgcggaatat ggaactggga acccagtttg tgcgccattg
     7621 agtgacatac acccaggaag gcgttcgcaa acgcgatacc cgcgatagtc gctgcactgt
     7681 gaacacgttc acgcgctacc ggatttttag acccttcgtg gtaggacgct ggcagatatt
     7741 ctttcagcag tttcagtgcc tgcagagcct gaccatcaga gaactcagat gccagtacag
     7801 aaacataagc ttccatggcg tgagttactg cgtccagacc accgaaagca cacagggact
     7861 tcggcatgtc cataaccagg ttggcgtcga caatcgccat atccggggtc agcgcatagt
     7921 ctgccagcgg atatttctga ccagtagcgt cgtcagttac aaccgcaaac ggagtgactt
     7981 cagaacctgt accagaagtg gtggtgacag cgatcatttt cgctttcacg cccattttcg
     8041 ggaacttgta gatacgttta cggatatcca taaagcgcag cgccagctct tcgaagtgag
     8101 tttccggatg ttcgtacata acccacatga tcttcgcggc gtccatcggg gaaccaccac
     8161 ccagcgcgat aatcacgtct ggtttgaagg agtttgccag ttctgcacct ttacgaacga
     8221 tgctcagggt cgggtcagct tctacttcga agaagacttc agtttcaacg cctgctgctt
     8281 tcagtacgga agtgatctga tcagcataac cattgttaaa caggaagcgg tcagtcacga
     8341 tgagcgcacg tttgtggcca tcagtaatca cttcatccag cgcgattggc agggagccac
     8401 ggcggaagta gatagatttc ggaagtttgt gccacaacat gttttcagct cgcttagcaa
     8461 cggttttctt gttgatcagg tgtttcggac caacgttttc agagatggag ttaccacccc
     8521 aggaaccaca acccagagtc agggaaggtg cgagtttgaa gttatacagg tcaccgatac
     8581 caccctgaga agccggggtg ttaatcagga tacgagccgt tttcattttc tgaccgaagt
     8641 aagaaacgcg agccggttgg ttatcctggt cagtgtacag gcaagaggta tgaccgatac
     8701 cacccatagc aaccagtttc tctgcttttt ctaccgcgtc ttcgaaatct ttagcgcggt
     8761 acattgccag agtcggggac agtttttcat gtgcgaacgg ttcgctttca tcaacaacgg
     8821 tcacttcacc gatcagaatc ttggtgtttt ctggtacaga gaagcctgcc agttcagcaa
     8881 ttttataggc tggctgacca acgatagccg cgttcagcgc accgtttttc aggataacgt
     8941 cctgaacagc tttcagctct ttaccctgca acagatagcc gccgtgggtt gcgaaacgtt
     9001 cacgtactgc gtcataaaca gagtcaacaa caacaacaga ctgttcagaa gcacagatta
     9061 cgccgttgtc gaaggttttg gacatcagta cagatgcaac tgcacgtttg atatcagcag
     9121 tttcatcaat aacaactgga gtgttacccg cgcctacgcc gatagctggt ttaccggagc
     9181 tgtatgcggc tttaaccatg cccggaccac cagtcgcgag gatcaggttg atgtctgggt
     9241 ggtgcatcag tgcgttagac agttcaacag aaggttgatc gatccagccg atcagatctt
     9301 tcggagcacc ggcagcgata gcagcctgca gaacgatatc agccgctttg ttggtggcat
     9361 cttttgcacg cgggtgcggg gagaagataa tggcgttacg ggtcttcaga ctgatcagcg
     9421 atttgaagat agcagttgaa gtcgggttag tggtcggaac gataccgcaa ataataccga
     9481 ttggttcagc gatagtgatg gtaccaaaag tgtcgtcttc agacagaaca ccacaggttt
     9541 tttcatcttt ataggcgttg tagatatatt cagaagcaaa gtggtttttg atcactttat
     9601 cttcgacgat acccatgccg gattcggcaa cggccatttt cgcgagtggg attcgagcat
     9661 ctgcagcagc cagagcggcg gcgcggaaga ttttgtctac ttgctcttga gtgaaactgg
     9721 catattcacg ctgggctttt tttacacgct ctacgagtgc gttaagttca gcgacattag
     9781 taacagccat aatgctctcc tgataatgtt aaactttttt agtaaatcat ctgctcgaat
     9841 acgagagtat agtcagtgcg gtgatgattt gcttaaccga tgaaaatcaa aagcttactc
     9901 gcgctcacac tcactgtgat ttactaaaag agtttaaaca tcagagttat tatctctaat
     9961 gcgtcacttc caggtggcgt aagcaagatt actcacttct gggtactgat tacgtgatcc
    10021 aaatcaaatt tttgcaaagc tgacaccttt cagcatcgct tttcgccatt atagctaaca
    10081 gttaataaat tgtagtatga tttggtggct acattagcat gttttgcaca actagataac
    10141 aataacgaat gatagcaatt ttaagtagtt aggaggtgaa aaatgctgtc aaaaggcgta
    10201 ttgtcagcgc gtcttttcaa ccttatttat ggctaacatt atccggcttt tgcttcggag
    10261 ctaaccgtga ttcagaccct ttttgatttt cccgtttact tcaaattttt catcgggtta
    10321 tttgcgctgg tcaacccggt agggattatt cccgtcttta tcagcatgac cagttttcag
    10381 acagcggcag cgcgaaacaa aactaacctt acagccaacc tgtctgtggc cattatcttg
    10441 tggatctcgc tttttctcgg cgacacgatt ctacaacttt ttggtatatc aattgattcg
    10501 ttccgtatcg ccgggggtat cctggtggtg acaatagcga tgtcgatgat cagcggcaag
    10561 cttggcgagg ataaacagaa caagcaagaa aaatcagaaa ccgcggtacg tgaaagcatt
    10621 ggtgtggtgc cactggcgtt gccgttgatg gcggggccag gggcgatcag ttctaccatc
    10681 gtctggggta cgcgttatca cagcattagc tatctgtttg gtttctttgt ggctattgca
    10741 ttgttcgctt tatgttgttg gggattgttc cgcatggcac cgtggctggt acgggtttta
    10801 cgccagaccg gcatcaacgt gattacgcgt attatggggc tattgctgat ggcattgggg
    10861 attgaattta tcgttactgg tattaagggg attttccccg gcctgcttaa ttaattcctt
    10921 tcaaatgaaa cggagctgcc atgctccgtt tacttcgtca ttatttttac tttgttcccg
    10981 cgcagttatc aaaagcaaaa ggaataggta aaaatattct tctcaaatta cagttagtta
    11041 taaggatttc cttaactgtt tctcttcacc atcatgttat tttcgccaca tcataatcct
    11101 gggcttgctg aagaataatt gaaatgatat tagtaattcc actgcctttg gtagaggaaa
    11161 gtgctaaata ataatcaatt gttaaatgat tgtgcatttc actactggaa ctgtaatcag
    11221 aaaagataga catgcttaac caatctctat ttgattgaat tgaaagatgt ttgttaagac
    11281 atggatgcaa gctatagatt ctgatacggt caataaaaga gaattgctta acaattttgc
    11341 aaaatgtatt ggcgagtaag aagcgcattt ggtactttcc gggcaaccgc caggcgattc
    11401 tttattggta atgagaataa ttaacaatta aagagcgtcg cgaaagaata atgtgtctcg
    11461 acaggggaga cacagtacga atcgacataa ggtgatcgtc tgaatcacca gaataaataa
    11521 agtcggtgat agcaatccgt aacgataaag taacctgaca gcagaaagtc tccgagcctg
    11581 tgcagggtcc cattccggga ttacacatgc tggttaatac cagtaattat aatgagggag
    11641 tccaaaaaac aatgaccaac atcaccaaga gaagtttagt agcagctggc gttctggctg
    11701 cgctaatggc agggaatgtc gcgctggcag ctgatgtacc cgcaggcgtc acactggcgg
    11761 aaaaacaaac gctggtacgt aacaatggtt cagaagttca gtcattagat ccgcacaaaa
    11821 ttgaaggtgt tccggagtct aatatcagcc gagacctgtt tgaaggctta ctggtcagcg
    11881 atcttgacgg ccatccggca cctggcgtcg ctgaatcctg ggataataaa gacgcgaaag
    11941 tctggacctt ccatttgcgt aaagatgcga aatggtctga tggcacgcca gtcacagcac
    12001 aagactttgt gtatagctgg caacgttctg ttgatccgaa caccgcttct ccgtatgcca
    12061 gttatctgca atatgggcat atcgccggta ttgatgaaat tcttgaaggg aaaaaaccga
    12121 ttaccgatct cggcgtgaaa gctattgatg atcacacatt agaagtcacc ttaagtgaac
    12181 ccgttccgta cttctataaa ttacttgttc acccatcaac ttcaccggtg ccaaaagccg
    12241 ctatcgagaa attcggcgaa aaatggaccc agcctggtaa tatcgtcacc aacggtgcct
    12301 ataccttaaa agattgggtc gtaaacgaac gaatcgttct tgaacgcagc ccaacctact
    12361 ggaacaacgc gaaaaccgtt attaaccagg taacctattt gcctattgct tctgaagtta
    12421 ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac ttataacaac atgccgatcg
    12481 aattgttcca gaagctgaaa aaagagatcc cggacgaagt tcacgttgat ccatacctgt
    12541 gcacttacta ttacgaaatt aacaaccaga aaccgccatt caacgatgtg cgtgtgcgta
    12601 ccgcactgaa actaggtatg gaccgcgata tcattgttaa taaagtgaaa gcgcagggcg
    12661 acatgcccgc ctatggttac actccaccgt atactgatgg cgcaaaattg actcagccag
    12721 aatggtttgg ctggagccag gaaaaacgta acgaagaagc gaaaaaactg ctggctgaag
    12781 cgggttatac cgcagacaaa ccgttgacca tcaacctgct gtataacacc tccgatctgc
    12841 ataaaaagct ggcgattgct gcctcttcat tgtggaagaa aaacattggt gtaaacgtca
    12901 aactggttaa ccaggagtgg aaaacgttcc tcgacacccg tcaccagggt acttttgatg
    12961 tggcccgtgc aggctggtgt gctgactaca acgaaccaac ttccttcctg aacaccatgc
    13021 tgtcgaacag ctcgatgaat accgcgcatt ataagagccc ggcctttgac agcattatgg
    13081 cggaaacgct gaaagtgact gacgaggcgc agcgcacagc tctgtacact aaagcagaac
    13141 aacagctgga taaggattcg gccattgttc ctgtttatta ctacgtgaat gcgcgtctgg
    13201 tgaaaccgtg ggttggtggt tataccggca aagatccgct ggataatacc tacacccgga
    13261 atatgtacat tgtgaagcac tgatggcaat acgtggggca ggagtgtcct gctccacggt
    13321 gtctgatttt tatcgcatta cagaaggcac aggccagaag gtagggcaat gttaaaattt
    13381 attttacgtc gctgtctgga agcgattccg acgctattta ttcttattac tatttcgttc
    13441 tttatgatgc gcctcgcgcc gggaagtcct tttaccggcg aacgtacttt accgccagaa
    13501 gtgatggcca atatcgaagc gaaatatcat cttaatgatc cgatcatgac acagtatttc
    13561 agctacctga aacaactggc gcacggtgat ttcggtccat cgtttaaata taaagattat
    13621 tcggtcaatg acctggtggc atccagtttt cccgtttctg ccaaactggg agccgcagca
    13681 tttttccttg cggtaatact gggtgttagt gctggcgtta ttgccgcatt aaaacaaaac
    13741 accaaatggg actataccgt gatggggctg gcaatgaccg gggttgttat ccccagtttt
    13801 gtggttgcgc cattattagt catgatattt gcgatcattt tgcattggct gccgggcggt
    13861 ggctggaatg gtggggcgct taaattcatg atattgccga tggtggcgtt gtcactcgcc
    13921 tatatcgcca gtattgcgcg tattacccgt ggctctatga ttgaagtatt acactccaac
    13981 tttattcgta ctgcccgggc gaaagggtta cctatgcggc ggatcatttt acgccacgca
    14041 ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc ccgcatttgt cggcattatt
    14101 accggttcta tggttatcga aaccatttat ggtttgccgg ggattgggca actgttcgtt
    14161 aatggcgcat tgaaccgtga ctattcctta gtgttaagcc tgaccatcct ggttggtgcg
    14221 ttaaccattt tgtttaatgc cattgtcgat gtgctatatg cggttatcga cccgaaaatc
    14281 cgttactgat actggagctc gcgatgatgt taagtaagaa aaacagcgag acgctggaaa
    14341 atttcagtga aaagctggag gtcgaagggc gcagcttgtg gcaggacgca cgtcgacgtt
    14401 ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct ggtgctgatc gcgttatttg
    14461 taatcctggc accgatgctt tcgcagtttg cctatgacga tactgactgg gcgatgatgt
    14521 ccagcgcccc ggatatggag tccggtcact actttggtac tgactcatcc ggtcgcgacc
    14581 tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat ggtcggtgtt gctgcggcac
    14641 tggtggcagt ggtcgtgggg acactttacg gttcgctttc cggttatctg ggcggtaaag
    14701 tggattcggt aatgatgcgt ctgctggaaa tcctcaactc cttcccattc atgttcttcg
    14761 tcattttgct ggtgaccttt ttcggccaaa acatcctgct gattttcgtg gcgattggca
    14821 tggtttcctg gctggatatg gctcgtattg tgcgtgggca aaccctgagt ctgaagcgca
    14881 aagagtttat tgaggcggca caagttggcg gtgtatcgac gccgggcatt gttattcgcc
    14941 acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc atcgctactg gtgcccagca
    15001 tgatcctctt tgaatctttc cttagcttcc tggggttggg tacgcaagag ccgttaagca
    15061 gctggggggc attgctgagt gatggcgcga actcgatgga agtctctcca tggttactgt
    15121 tgttcccagc gggattcctc gtggtgacgc tgttttgttt caactttatc ggcgatggct
    15181 tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca gccatgagcg taattgaaac
    15241 tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg ctgaacgtga aagatttgcg
    15301 tgtcaccttt agtaccccgg acggcgacgt cacggcggtc aatgatttaa atttttccct
    15361 acgtgccgga gaaacgctgg gcattgtagg tgagtctggt tcgggtaaat cgcaaactgc
    15421 atttgcgttg atgggcctgt tggctgccaa cggacgcatt ggcggatcgg caaccttcaa
    15481 tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat aaactgcgcg ctgaacaaat
    15541 ctcaatgatt tttcaggacc caatgacttc gttgaatccc tatatgcgcg tcggtgagca
    15601 gttgatggaa gtgctgatgc tacataagaa catgagcaaa gctgaggcgt ttgaagagtc
    15661 ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt aaacgcatga aaatgtaccc
    15721 gcacgaattt tctggtggca tgcgtcagcg agttatgatt gcgatggcat tgctatgtcg
    15781 acctaagctg ctgattgcag atgaacccac tactgcgctg gacgtcaccg tacaggcgca
    15841 gatcatgacg ctattgaatg aactgaagcg ggaatttaat accgccatca ttatgatcac
    15901 ccacgatctt ggcgtggtgg cggggatctg cgacaaagtg ctggtaatgt acgccgggcg
    15961 cacgatggaa tatggcaacg cgcgcgatgt cttttatcaa cccgttcatc cttattctat
    16021 cggtttgctc aacgcggtgc ctcgtctcga tgcggaaggt gaaacaatgt tgactattcc
    16081 gggtaatccg ccaaacctgc tgcgattacc gaaaggttgc ccgttccagc cacgttgtcc
    16141 gcatgcgatg gaaatttgta gtagcgcacc gccgctggaa gagtttacgc ctggccgtct
    16201 gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg ctgtaaccga aggaagaaaa
    16261 gtcctcctcg aaatcgacga tctgaaagtg cactttgaaa tcaaagatgg caaacagtgg
    16321 ttctggcaac caccgaaaac tctcaaagcc gtagatggtg taactcttcg cctgtatgaa
    16381 ggggaaacat taggtgtggt aggggaatcg ggatgcggta agtccacctt tgctcgcgcc
    16441 atcatcggtt tggtcaaggc gaccgacggt catgttgcct ggttaggtaa agagttgctg
    16501 ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata ttcagatgat tttccaggat
    16561 ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga tcattgctga accactgcgt
    16621 acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc gcgtgaaggc gatgatgctg
    16681 aaagtcgggt tattgcctaa cctgattaac cgctatccgc atgagttctc cggtgggcag
    16741 tgccagcgta tcgggattgc tcgtgctctt attcttgaac cgaagctgat tatctgcgat
    16801 gagccggttt cggcgctgga tgtgtcaatt caggcgcagg tggtcaacct gctccagcag
    16861 ctgcaacgtg agatgggatt gtcattaatt tttattgctc atgacctggc cgtggtaaaa
    16921 cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg cggtagaact ggggacctat
    16981 gatgaggtct accacaatcc actacatcct tacaccaggg cattgatgtc ggcagtcccc
    17041 atacctgatc cggatctgga gaagaacaaa accatccagt tactggaagg ggaattaccg
    17101 tcgccgatca acccgccttc cggttgtgtt tttcgtaccc gttgcccgat tgccggtccg
    17161 gagtgtgcca aaacacgtcc tgtgctggag gggagtttca gacacgccgt ttcttgcctg
    17221 aaagtcgatc cgctttaaac acaaaaaggg ctgacagttg tcagcccttt ttcacgccga
    17281 aagtgattat ttattcccgc cagatgatat ggcaaagttt gtgatctttt tcgcggcata
    17341 acagaatgcg ggcgaaaaca tcgttgattt caccatcttc actgtccgcc agaccaatca
    17401 ccacttcggc aaaaaagtct gggttcaaat cgaaatcaac atgttcctgc cagtcttccg
    17461 ccggatcgaa taactccgcg ccgccgcgct cttcaaactg aagattgaac agcagaacat
    17521 cagctggatc gagattgtcg gcagccagtt cgagaaaaat atcgtaggcc tgctcaagcg
    17581 tttcatcttc agtcaggcga ttgtttagat ccatatccat agtcactacc tgtttaacct
    17641 ctgttggcga cgttttacag caacgggctg aagaagtaaa acagtcgctc ggcgacacgt
    17701 tgccataatg gacgttttag ccataaacgg gcatcgagca gacgtgaacg cgaaatataa
    17761 tcgtcctgaa cggcggcaag gtctgcacca aaacctttat cgtcgattgc cagggtaatc
    17821 tcgaaattca gccacagact acgcatatca aggttaacgg tgccaaccag acttagttcg
    17881 ccatcgacca gcacgctctt ggtatgcagt aacccgcctt caaactgata aattttaacc
    17941 ccagcagcaa gcagttccgt aaagaatgcg cgactggccc agccgaccag catcgagtca
    18001 ttttttcgcg gaaggataat actgacatcc accccgcgct gcgccgccgt gcaaatcgca
    18061 tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca tgatcaaata ttcacgcgcc
    18121 gaataagccg cagtcaataa tgcctggtga atgagatctt ccggaaagcc ggggccagaa
    18181 gcaattgtgt gaatggtatg accgctggcc tgctcaaacg gcataatatt gacatcaggt
    18241 ggtggcggta gaatacgttt ccctgtttca atctcccagt cgcaggaata aataatcccc
    18301 atcgcggtgg cgatggggcc ttccatacgc gccatcagat caatccattg ccctacgccc
    18361 gcatcttgtt tgaagtagcg aggatcgacc atattcatgc tgccggtgta cgcgatgtaa
    18421 ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca tacggcgtaa aaacacacgc
    18481 atcagattga cctttaaggc ttcgaccact tcaataccgg cattacgcat tagctcgggc
    18541 cacgggctgc ggaaaaaagc cacactcccg gcggagtcga gcatcaatcg gcaatgaatg
    18601 ccgcgtcgcg cagccgccat taatgattca gccacctggt ccgccatgcc gccgggctgc
    18661 cagatataaa acaccatctc aatattatgg cgcgcgagct ggatgtcgcg gattaacgcc
    18721 tgcatcacat catctgactc ggtcatcagt tgtagctgat tccctttgac cccagcgatc
    18781 ccctgacgac gctcgcaaag cttgaataat ggcgcagcga cactgctatt ttcttcggcg
    18841 aagatatgct tacaggcttt aaggtcgtta agccattttg cggtggaagg ccacatcgct
    18901 ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc caacggcaag ataggcaata
    18961 attccgacta acggcagaat gtaaataatc aacagccagg ccatcgcgga gggaactgcg
    19021 cgtcgtttca ttagaatgcg taaagttacg cctgcaatga gcaaccagta tcccagaatg
    19081 gccaaccaac tcaccaacgt ataaacggtt gtcatagatt agaaatcctt ttgaaagcgc
    19141 attgttatga gtttacgcat atggattcat ctggcaaata aaaacgcggg aaaagcactg
    19201 gtcagcgtcg ggggcggggg tataatgacc attctgttat tgcatagagt agttaacatg
    19261 aagcggagta gaacggaagt ggggcgctgg cgcatgcagc gtcaggctag ccgacgtaaa
    19321 tcgcgttggc ttgaggggca atcgcgccga aatatgcgta tccacagcat caggaagtgc
    19381 attctaaaca aacagcgtaa ctcgttattg tttgcgatct acaatatcta aatgttctgg
    19441 gcaccattgc ggtgcccaat aagtttactt ttccgtcttt aactctattt ttgcaccgat
    19501 tctttactgt cagctttcgt ttcttgttca tcgttatcgc tgaaaatgcc atgaccggag
    19561 ttcaacaaca ttgaaacgag catatcatta ttaatctctt caccatttaa aactcgcgcc
    19621 agaatttcgc tgccaaacag ttgcggtgag agaatgatat ccggatgtac catcttaatc
    19681 ttattcaggt ttttgctatc actgacggcg agaactgttt tgacatcact gctcatatct
    19741 tttgccgaga gtacaacaaa cgcgttatct gcatcgttat cactcagcgc cagaatggcc
    19801 cggcaacgat cgattcccgc tttctttaat acagaactgt cattactgtc accggggata
    19861 acgtctgcgt tatcgcctaa gcgttgctca agttgcttga tatcatcttc aggcaagttg
    19921 ctgataaccg ttacgttttg tccgcgttga ttcagttgca ggatcgtatt gatggcgaga
    19981 atcgaatgtc cgcaaacaat aaaatgatct ttacgatgca ttgtatgatt gtttcctttt
    20041 acaagtttgt tgaatccccc gcggataagc ggaccaaaaa ttgaggtcat agatgtggca
    20101 aaaacggtaa tgccggaaat aatgaccgaa atagtgaaca atcgtgctga ttcagaaaca
    20161 gggacaatat cgccatagcc gacggttgac atggtttcta tcgaaaaata gaacgcggtc
    20221 atcaaacttt ctattcgcgg attaaaacct tcgcttaaat aaagcgcacc gtaggttgag
    20281 taaaacagta atgtcgtgaa actaataaat gcaaaaattg tcccggctgc ggcgctactg
    20341 tgggagaagt ctttgcgcag tatcagcaaa aacaccagcg taaaaatgca aaatccaata
    20401 ctaaatttca gccagggata aaaatgcagg gtgtaaatta gcgctatcaa caacaaaatg
    20461 atactgattg cccatgcgag ctttgcccgg aacagtaagc caatggagtt caggacaaga
    20521 aagacgccga gcataaatag tggggcgttt gccagcaagg agagatccaa ctctgaaaat
    20581 gctttgatat ggaaaatatc aagcaaattg accgacatac catagattgt tttaaaaata
    20641 agcaatccat ttaaaaagac ggccagcgcg agaatgtcgt gccgtagggt cacccataaa
    20701 tttgttgctg tttgtttgaa tgtagcccag tgactcactg atacctctgt aaccacaatt
    20761 ttgtttaatt atcttagcgt gcacattttc tgaacataca tgcagcgcgt gaatgtgtta
    20821 aacaatagta gatgacttta tccgcagtaa tcaaaggttt tgagtgagca actctgcgtc
    20881 aggaaagggc atttttcctg aatatgctca catcatataa agaaatacag ataaagttat
    20941 tatctgcttg tggtggtgaa tgcactgacc gacctataag gaaaggccaa acaagaacac
    21001 ggttgcaaaa accgtgccct taaatattgg ctctctactc agaacacttt cttaaatggt
    21061 ttcactgaaa cgtgttcata gactcctgcc gctacgtacg ggtcagcatc ggcccaggcc
    21121 tgagctgctt ccagcgattc aaattcagca ataacggttg agccagtaaa tcccgcagcc
    21181 cctggatcgt tactgtctac cgctggcatt ggaccagctg tcaacaaacg accttcatca
    21241 tgcagtaact gtaaacgtgc taaatgtgcc ggacgaacgg aaaggcgttt ttcgaggcta
    21301 tcagctttgt cttgagcgta aataacatac aacacgggca caactccttg ttcgggaaag
    21361 ttgtaaagta cgttatttga aagggcgaag atctgcaacg gaaagatgat gtctttgtta
    21421 agaccatgca taaagtaagg tcaattacgc caaaaatgac attttcactg atcctgatcg
    21481 tcttgcctta ttgaatatga ttgctatttg catttaaaat cgaggcctgg tttttctact
    21541 gaaatgatta tgacttcaat gacccttgat ttacctcgcc gcttcccctg gccgacgtta
    21601 ctttcggtct gcatacatgg tgctgttgtg gcgggtctgc tctatacctc ggtacatcag
    21661 gttattgaac tacctgcgcc tgcgcagcca atttctgtca cgatggttgc gcctgctgat
    21721 ctcgaaccgc cacaagccgt tcagccgcca ccggagccgg tggtagagcc agaaccggaa
    21781 cctgagccga tccccgaacc gccaaaagaa gcaccggtgg tcattgaaaa gccgaagccg
    21841 aaacctaagc caaaaccgaa gccggtgaaa aaggtacagg agcagccaaa acgcgatgtc
    21901 aaacccgtag agtcgcgtcc ggcatcaccg tttgaaaata cggcatcggc acgcccgaca
    21961 tcaagtacgg caacggctgc aaccagcaag ccggttacca gtgtggcttc aggaccacgc
    22021 gcattaagcc gtaatcagcc gcagtatccg gcacgagcac aggcattgcg cattgaaggg
    22081 caggttaaag ttaaatttga tgtcacgccg gatggtcgcg tggataacgt gcaaatcctc
    22141 tcagctaaac ctgcgaacat gtttgagcgt gaggtgaaaa atgcgatgcg cagatggcgt
    22201 tatgagccgg gtaagccagg cagtgggatt gtggtgaata tcctgtttaa aattaacggc
    22261 accaccgaaa ttcagtaagc agaaagtcaa aagcctccga ccggaggctt ttgattatta
    22321 ctcaacaggt aaggcgcgag gttttccttc aggatcaacc gcgacatact taaataatgc
    22381 ttctgtcgct ttatagcgtt gcccgattgg ttcagacgct acttttttca cccacacttc
    22441 aatattaatg ctgaccgatg tcgtcccttt ctggacacag cgtgcatagc agcacaccac
    22501 atcgccgacc gcaaccggcc gtaagaaagt cattccttca acccgcacag tcactacgcg
    22561 accgtgggca atttctttcg ccagaatagc gccgccaata tccatttgtg acattaacca
    22621 accaccaaag atgtcaccat tggcattggt atcggcgggc atggctaaag tacgtaaaac
    22681 aagatcgccc tgagggacgt tatgtgttgt agacatggta aaaccgactt aaaaggaaaa
    22741 tcacaggcgc gatgctacta tgattttcct gaggagaaca gaggagatac agcgccagcc
    22801 ccgaaggact ggcagtctgg ttaggattta tcttcctgcg gcatgtggcg gtagatatag
    22861 ataccgctta acaatgtgaa gattaaggta agggcggtca ggccaaagac tttaaagttg
    22921 acccaaatat tttgcggcag ccagaatgcg atgtagatgt ttgccagacc gcaaaggata
    22981 aagaaaacag cccaggccag attcagcttc gaccataccg gttgcggcag cgtgagttct
    23041 ttacccagca tccgctgaat tagcggcttt ttcatcaccc attggctgac taacagggca
    23101 cccgcaaaca gggcataaat gacagtaacc ttccatttaa taaactcatc attgtggaag
    23161 aacagcgtca agccaccgaa gacgaccacc agaacaaaag tgatcagggc catcttctca
    23221 accttacgaa agcgaaccca gctatagata agcacaatcg ccgtggcgac gatcagcgcc
    23281 gcagtagccg catagatgtc ataaatcttg taaaacgcga aaaagacaac cagcggtaaa
    23341 aaatcaagaa actgcttcat tttacgattc cgtaatcaaa tgcttaaggt tattggcgaa
    23401 tcaacatata cagacggaac agatagatga gcaatacggc tgaaatcagg ttgctcaagg
    23461 tgttcgccag tacggcacca atttccgggg ttaatgcggc aaaagaagag gcaaaaagca
    23521 gcaacagtgt ctttgccagc aaccagctca gtactgcggg cgccaccaga cgcatattcg
    23581 cccaagtcag ccgcatactg ctacgcatcg aggcaaaaac gcccattttg tcctgtacca
    23641 acatcaccgg tgccagggcc agtaaaatgg ccatgataat tcccggaacg accactagca
    23701 tgatgccaat ctgtactaaa agggtagtca gaaaaatcag aataaataac tttggcaata
    23761 tcggcgcact ggcaccaata gctcgcagcg cactgactct ctgacccgca gacaccagct
    23821 ggataattaa tattacgcct ccggcgagaa tggcgttacc gattaatcct gaaaaagtgg
    23881 acgccgctga agcctgcagc aaaatttgtt gctgttccgg tgacatattc tgaaccaggt
    23941 cgaacaaccc actactgccg ctaacgggca cgccgtcatt gagctgcgca agctgtgcat
    24001 cactgggtga gaaaacatgc cctaacacca ctgtgataaa cgcacatagc aacgatacca
    24061 acagaatggt cataaattga ttacggaaga aatttccggt gtcacggtat acggactgcg
    24121 ccgtgataga catgcactct ccttgagttt tatgcaggtg ttaattagcg ggcaattgta
    24181 ccctggttaa gcctcgggtg gcagcatcag gtcttgtatg gaaaagcata tctttgtaaa
    24241 gcggaggtaa ttgctggcct tcaggcgtgg gcaaaccgcg gcaagtggcg atcgagttaa
    24301 tatttgcgta gcgaaaatat ttaaaaattg atttaaatca cgttaaccag gattcccaat
    24361 gcaacttcta aattaatcca gatcaataaa gggtgaatta tcatatgtaa tgtgatctat
    24421 gtaggatcat ttgttactcc aatgtaggta tatttgtcac gtttttataa ccataacgac
    24481 ggagcggata tgaaaaagtt aacagtggcg gctttggcag taacaacact tctctctggc
    24541 agtgcctttg cgcatgaagc aggcgaattt tttatgcgtg caggttctgc aaccgtacgt
    24601 ccaacagaag gtgctggtgg tacgttagga agtctgggtg gattcagcgt gaccaataac
    24661 actcaactgg gcctgacgtt tacttatatg gcgaccgaca acattggtgt ggaattactg
    24721 gcagcgacgc cgttccgcca taaaatcggc acccgggcga ccggcgatat tgcaaccgtt
    24781 catcatctgc cgccaacact gatggcgcag tggtattttg gtgatgccag cagcaaattc
    24841 cgtccttatg ttggggcagg tattaactac accaccttct ttgataatgg atttaacgat
    24901 catggcaaag aggcggggct ttccgagctc agtctgaaag attcctgggg agctgccggg
    24961 caggtggggg ttgattatct gattaaccgt gactggttgg ttaatatgtc agtgtggtac
    25021 atggatatcg ataccaccgc caattataaa ctgggcggtg cacagcaaca tgatagcgta
    25081 cgcctcgatc cgtgggtgtt tatgttctca gcaggatatc gtttttaatt ccgcacaaaa
    25141 acgaccccgt aatatacggg gtcaataagg acatggcata aagggtatta tttcttcgct
    25201 tctacgccat cagtttcaga gcgaattaaa aatttctcag ttgtttgcgg aatatgctga
    25261 atcagccagt cggccatttg cttttcctca tttaaaatcg cttcgatgat cggaattgag
    25321 gctgtatcac cggcattttt tgctgctgct aatagagatg tgtaacaggc gatttcaaat
    25381 tgctcgaaga catatccgct aatagaacct ttgactattt catcagaagg gaatatacca
    25441 ccgattgact gcccaagcgc agccatttta ctcatggaat ctttaatgac tgaacgtgaa
    25501 atgtcattac gatcaagaat agtttccagt tgaacaatct ggtttttggt ttcactaaga
    25561 tgttgttcaa tacgagcacg tagttcagga taattatcta tacggctggc catggattca
    25621 agcatagatt cggcttgctt ttccattgca tgggcgtcac gtaaccagtc atgataatgt
    25681 tcaatacgat tcatgaaaat gtcctcttta taaaatgact aaaagttaaa attcatattt
    25741 caggctttat tttcggcttt cttatttacg ttattaaggg ccagatcagt cagtttgatg
    25801 tcggtggcct tttcttcttc cagggtctct ttcagaagct tcgctgcttt acggtaacct
    25861 aattgttcag ccagcgtcgc taatgtcccg taactggcaa tctcataatg ctcgactttc
    25921 tgtgctgcgg caatcagtgc ggcatcacgc acttcgtttt tctcggtact ttcgatgacc
    25981 tcattagctt cttcaataag accttccatt gccacacatt tcatgcgctt aattttcaga
    26041 ttcgattctg attccacaac ttgatcaata cgttcaatct gtccatgtgt ttcctcgagg
    26101 tgcgcatgaa aagcctgact taatttttcg tttgatgttg ctcttgcgag ttttgccagt
    26161 gcccgggtta attgtttttc tgcgctgtag gtatctgaaa gtaggtgaat aaatacatct
    26221 tccatggtct tcatattcat atttttctcc agtggaatca ctgcgagcca tacggcccgc
    26281 agaagcaatt atctttatca gctgatatta ataattaacg gtgaatcagg atttacctga
    26341 tttattacca ccgttttgtt gaccgccttt tttacccgct tcagatgcgc gttgcggatc
    26401 atttttaaaa ttaccgccgc tatgctgacc gcctttacgg cctgcgtcgg atgccttctc
    26461 acggtcttcg gcgaaatttc ctgaaccacc acgatgttcg gccatgttat ttctcccgtt
    26521 gcgttgcatt gtttcattaa tatgagtgtt gtgtgtcgac actcattaaa attagtcgat
    26581 aatgagaatt ggtcaaatta agcgtaacga gaaggaagtg ggaaaatata ttatgaggaa
    26641 cgttcttgat atattaacaa ttgcctgagg aataagtgac ttagagaaaa ttattgattt
    26701 tactggtgtt atgttgcggg attaatttgt ttgattatat tactgttggg cggaaaaatg
    26761 acgtaagttg acgttcgaca ggggtaagcg aaacggtaaa aagataaata ttaaatgaat
    26821 ttaggatttt tccggcttcg ttaaagaaag ttaaaatgcc gccagcggaa ctggcggctg
    26881 tgggattaac tgcgcgtcgc cgctttcatc ggttgtacaa aagctttcag tgccgccagc
    26941 attttctctg gctcattaat atgttgctcg atgattttaa caatggccga accagaaatc
    27001 gcgcccgcag ctcctgcatc aatcgctgct tttacctgat ccggggcgga aataccaaat
    27061 ccctgcaatg gaggtgcagc gttgtactct ttcagcttcg caaccagatg attgaggggt
    27121 aacgcggcgc ggttttctgc gccggtcacg cccgctcgtg acagcaaata tgtgtaacca
    27181 cgaccgtaag aggctatctg gcgcagcagg tcgtcatcgg catttggcgg gcagatgaag
    27241 ataggtgcga cattatgacg caacgcggcc tggcggaagg gcgcggactc ttcaactggc
    27301 acatcggcaa ccagcaccga atcgacgccg actttttcgc actgggcata aaactcatca
    27361 atgcctttgt taaacaccag attggcatac atcaacaggc caatgggaat ggtcgggtgt
    27421 ttctggcgaa tcagtgccag catttcaaaa cattgtgccg gagtcacacc tgccgcaaag
    27481 gcgcgcagag tggcgttttg aatggtcggg ccatccgcca gtgggtcgga gaaggggata
    27541 cctaactcca gcgcgtcagc accggcttca attagcgtat cgataatttt caatgactgc
    27601 tcaatgcccg gatccccgag cgtgacgaaa ggaacgaatg cgccttcttt gcgctccttc
    27661 aactgggcaa acagagattc gtagcgttcc atcagatttc ccctcgtgct ttcaaaatat
    27721 cgtgaacggt gaagatgtct ttatcgccgc gaccggaaag gttaaccacc agtagctgct
    27781 ctttttccgg gttttcgcgc atcattttca gtgcatgggc cagggcgtgg gaggattcca
    27841 gcgccgggat gatcccttcg tgcaggcaca gcgttttgaa ggcttcaagg gcttcatcat
    27901 cggtaataga cacgtaatca gcgcgtccag tgctgttaag atacgcgtgt tgtgggccga
    27961 cggacgggaa atccagcccg gcggagatgg agtaagattc ttcaatctgc ccgtcttcgg
    28021 tttgcatcat cggcgctttc ataccgaaat agatgcccac gcgaccatgt tttaacggtg
    28081 cgccgtgctc gccggtttct ataccgtgac cgccaggctc cacaccaatc aggccgacgt
    28141 tggtttcgtt gatgaaatct gcaaacatgc cgatggcatt cgaaccgccg ccaacacagg
    28201 cgataacggc atccggcagg cgaccttctc tttccagaat ctgtgctttg gtttcttcgc
    28261 caatcatccg ctgaaactca cggacaatgg tcggataagg atgcgggcca gctgcggtgc
    28321 ccagcatata gtgcgcggtt tcataactgc cggaccagtc gcgtagcgcc tcgttacagg
    28381 catctttcag cgtcgcggaa ccgctatgca ccgggatcac ttccgcaccc attaagcgca
    28441 tacgaaaaac gtttggcgac tggcgttcaa cgtctttggc ccccatataa atacggcatt
    28501 tcaggccgag cagggcgctg gcaagggccg acgccacgcc atgctgaccg gccccggttt
    28561 cggcgatgat ttcggtttta cccatccgct tcgccagtaa agcctgaccg agcacctggt
    28621 tagttttatg cgcgccgccg tgcagcaaat cttcgcgctt cagatacagc gtggttttcg
    28681 tcccagccgt aatgttctgg catttggtca gtgcggttgg acgcccggca tagtttttca
    28741 gtaggtcgtt gaactgggcc tgaaattcag gatctttttg cgcgctgaca aaagcttctt
    28801 ccagctggcg cagagcaggc atcaggattt gtggcacgta catgccgcca aactcaccaa
    28861 aataggggtt aagtaatgtt gtcattgttc ctttccttaa tatgcgcgca gcgtctggaa
    28921 aaccgaggcc aaaagacgtg cgtctttgat gcccggttgc gactctacag cagaattaaa
    28981 atcaagtccg gcgcagccgg tttgtgccgc ttccacgcag ttatctgcgc ctaagccccc
    29041 cgccagcaga acgttgccaa gcgattgacc gtttaacagt gaccagtcga aacgttgccc
    29101 gcttccaccc tggccgttgt ctaaaacata tttatcaacg tgctgaagct cgcgggcggg
    29161 cagggtttca ccgacgctta atgctttcca gatggcaacg tgtgctggca gagcttcacg
    29221 cagcgtatcg atatacagct gatcttcatt accatgcagt tgcactgccg ccagcgataa
    29281 caccttagct ttgtccacca catcggcaat atcgtgattg cggaacacgc caacatactg
    29341 caacggtgcc gcagccatca cttcctgcgc ctgttcaatg ttgacgcaac gcggtgatgt
    29401 cgcaacaaaa atcaacccac cgtaaatcgc gcccgcgtca tacgctgctt tagcatcttg
    29461 cccacgcgtc aggccacata ctttattctc acccagcaac acccggcgca cggcggcgtt
    29521 caaatcgtca tgggccatca acgccgaacc aatcagaaaa ccgttagcga agtggcttaa
    29581 ctcgcgcacc tgagcgtaag tattgatgcc tgattcgctg attaccgtca cgttgtgccc
    29641 cagtttcggc gcaagctcgc gggtacggtt gagatcaatc gacaaatcac gcagatcgcg
    29701 gttgttgatg ccaacgacct ttgcccccaa tgcaatggcg cgctccagtt cctcttcatt
    29761 actgacttcg gtcagcacac ccatctccag actgtgggcg acggcggcaa gctggcgata
    29821 ttgttcgtca tccagtactg aaagcattaa taagcaggca tcggcctggt aatagcgcgc
    29881 cagatagatc tggtaagggt cgataatgaa gtctttacat aaaatcggct gcggggcgat
    29941 ttggctgacg atggggagga aatcaaagct cccctgaaaa tatttctcat cagtcagcac
    30001 cgaaattgcc gaagcgtaat gtttataaat ggcggcaatg cgtgctggat cgaaatcatc
    30061 acggatcacg ccttttgacg gcgacgcttt cttgcactcc agaataaacg ccgtgcgcgc
    30121 accctgtagc gcatcataaa aatgtcgcgt gctcggctga acctcattct gaaaactggc
    30181 cagcggttgc tgctgtttgc gggcttctac ccaaatcgcc ttgtctgcga cgatcttcgc
    30241 taaaacggtt tgcatcattt accctcgtgc cgccagtgcg gtgactctgt cgtaagcgga
    30301 accactgcgc agtacctcaa gaacggtttg cgcattggct tgcagatctt catggccatg
    30361 caggcgcatt aacatggcga cgttcgcagc gacggctgct tcatgggcgg cgtcgccttt
    30421 accttgtaac aaacgtgtta aaatgtcacg gttttcttcc ggtgttccgc ctgccagttg
    30481 ctcctggtgg tagggcgtca ggccaaaatc ttcagcggtc aattgatagc tcttaatttc
    30541 gccgtcatgc agctcggcaa cgattgtcgg cgcgtgtaat gaaacttcat ccatcccgcc
    30601 gctgtgcacc accgccgcgc gttgataccc cagcacgcgc aaggtttcgg caatcggcag
    30661 caccagttcc ggactataaa caccaattaa cgccagcggc ggatgcgccg ggttaatcaa
    30721 tggtcccagc acgttgaaca aggtgcgggt tttcagttgc tggcgaaccg gcatcgcgtg
    30781 gcggaatccg gtgtgatact tcggcgcaaa gaggaaacag acgcctaatt cgtccagcgc
    30841 ctggcgcgat ttatcggcgt tcatatcaag attaataccg aatgccgcca gcagatccga
    30901 cgagccagat ttactggaga cgctacggtt gccgtgtttc gcaactttca gcccacaggc
    30961 cgcggcgaca aacgcactgg cggtggaaat attgatactg ttgctgccgt caccgccagt
    31021 cccgacgata tcagcaaaca gataatccgg gcgcgggaac ggcgctgcgt tttccagtag
    31081 cgcggttgct gctccggcga tctcgttcgg gtgctcaccg cgaattttca tgctcaccag
    31141 cgccgccgcc agttgttccg gcttcagctc gccacgcacc accgctgaaa acagctggtg
    31201 actttcttgt tggctaagcg tctgcgcctg atacagtttt tccagaatcg gttgcagcgt
    31261 gttggttggc tcaagtttct gctgcgccca ggccagcgtt tgttccagca ggcgagcgcc
    31321 ctgggtagta agaatggatt ccggatggaa ctggaatcca caaacgcgat ccgcatcgtg
    31381 acgtactgcc atcaccatgc cattaaaatg ggcattgatg gttaaacctg ccggaatgtt
    31441 actgccaacc agcgagtgat aacgcgccac cggcaatggg tttgttaatc cggcaaacat
    31501 cgcctgaccg tcgtgttcaa tgctggaggc tttaccgtgg agaatttcgc ccgcctgacc
    31561 gacatagccc ccgtaagctt cgacaatcgc ctgatgacca aggcaaatgc caataatcgg
    31621 cagcttaccg cgcaggcggg taagcagttc tggcatacaa ccagcttcgc tcggcacacc
    31681 gggtcctgga gaaagcatca gcaccggatt gctcatcgtc gccaggcgtt caattaaggt
    31741 ttgcgccgga atatggttgc ggtaaatcac cacgttatgc ccattgctgc gcaactgatc
    31801 tgccaggttg tacgtaaaag agtcgatatt atcgagcagc agaatgtcag ccatcagaaa
    31861 gtctcctgtg catgatgcgc ggtggcaata gcgcgcagta cagcgcgggc tttattacgg
    31921 gtttcgtcgg cttccgactg cggaacagaa tcaaggacta cgccagcacc ggcttgcacg
    31981 gtggcgatac cgttttccac cagcgccgag cggatcacaa tgcaggtgtc gagatcgcca
    32041 tgcgcggtga aataacctac cgcgccgccg tagctgccgc ggcgacgacc ttcagcctcg
    32101 gcaattaact gcatagcgcg tactttcggc gcaccgctta acgtacccat attcatacag
    32161 gcgcggtaag cgtgtagggc gtcgagatcg tggcgcagct caccgaccac gcgggagacc
    32221 aggtgcatca cgtaagagta acggtcaact ttggtaagat cggcgacgta gcggctgccg
    32281 ggggtgcaaa tgcgtgccag atcattacgg gcgagatcca ccagcatcag atgttcagaa
    32341 agctctttat gatcggtacg catttccagt tcgatgcggc tgtcgaggtc tctgtccagt
    32401 gaaccatcgg cacgacgacc gcgtggacgt gttccggcaa tcgggtagat ctcaatctgg
    32461 cggctggtgg cgtcatactt gagcgaactt tccggcgacg cgccaaacag ggtgaaatca
    32521 ttatcctgca taaaaaacat gtacgggctg ggattactct ttttcagcac gtaataggcc
    32581 gccagcggtg acgggcaggg cagagagaaa cggcgagatg gcaccacctg gaaaatttct
    32641 ccggcgcgaa tcgctttttg caacaaacgc actacgccac cgaactcttc atcgctctgg
    32701 ttacattcac aacgcatatg cggcacggaa accaccggca gcggtggcgc ggtttcggtc
    32761 agttgctggc gaagatcgtt caggcgagca gtgagacgtt gtttttcttc ttcattcgga
    32821 gcaaaaaggc tggcctgaat gcgggtgctt tttttctgat ggtcaatcac catcagcgtt
    32881 tcagcgagat aaaaacagaa atcagggcag ttattttccg ctgacagttg cggtaaatct
    32941 tcaaaccccg ccacaaggtc ataagagaac aggccgccga agaacatggc ttctcgttct
    33001 tccttcggta cattcaacag attctgcaat aagcggaaag cgtcaaaaac cgaaagggag
    33061 cataagcgag cgtcttcatc cagcagtgga ctaacagggg ggaagctcag cacacggcag
    33121 tttggtaatt gttcattttc cacacccgca ggcagggcgt tatccagtag tgtcagcagg
    33181 gcttcgccgt tgccggaaag ggactggatt gtgacagtgt cacctaaagc tgtaatgcgc
    33241 agcgcactgt ctaccagcag caggcttttt aaatcatctt tgctgtcgat atctgcggat
    33301 tccagcagca gcgttgccgg acgattccca cacaactggt gaaaaagcgc ggtgggattg
    33361 tcgtgataag cgccttcgca ggttagcagt tcgagagtcg gtttttgtgt ttgcattgtt
    33421 attctctaat tttgttcaaa aaaaagcccg ctcattaggc gggctgggta tctgattgct
    33481 ttacgcatgg tgaatacact gcccgtttca ggaagtgcgc caccaacctt tcagtacgaa
    33541 aattgctttc attgtcgata ccctttttac gtgaacttgc gtactagtta actagttcga
    33601 tgattaattg tcaacagctc atttcagaat atttgccaga accgttatga tgtcggcgca
    33661 aaaaacatta tccagaatgg gagtgcgcct tgagcgacac gaattacgca gtgatttacg
    33721 acctgcacag ccataccaca gcttccgatg gctgcctgac gccagaagca ttggtgcacc
    33781 gtgcagtcga gatgcgcgtc ggcaccctgg cgatcaccga ccatgacacc acagctgcaa
    33841 tcgcgccagc aagagaggaa atttcacgtt cgggactggc actgaatctt attcccggcg
    33901 tggaaatatc cacggtctgg gaaaatcatg aaattcatat tgtcgggctg aatattgata
    33961 ttattcatcc gctaatgtgt gagttcctcg cgcagcagac agaacggcgc aatcagcggg
    34021 cgcagcaaat tgccgaaaga cttgaaaaag cgcaaatacc tggggcgctg gaaggcgcac
    34081 aacgactggc gcagggcggc gcagtgacgc gcggtcattt tgcgcgtttc cttgttgagt
    34141 gcggcaaagc cagttcaatg gcggatgtct ttaaaaagta tctggcgcgc gggaaaaccg
    34201 gatacgttcc gccacagtgg tgtacaatag aacaagctat tgatgtcatt catcattctg
    34261 gcggtaaggc ggtgctagct catcctgggc ggtacaatct tagtgctaaa tggctgaaaa
    34321 gattggttgc gcattttgcc gaacacgacg gcgacgcgat ggaagtcgcg cagtgccagc
    34381 aatcgcccaa tgaacgtacc cagctggcga cgcttgcccg ccagcaccat ttatgggcat
    34441 cacaaggatc tgattttcat cagccatgcc cgtggatcga actgggtcgt aaactctggc
    34501 tgcccgcagg cgttgaaggc gtctggcagc tatgggaaca gccgcagaac accacagaga
    34561 gggaattatg agccagtttt tttatattca tcctgataac ccgcagcaac gtctgatcaa
    34621 ccaggcggtg gagatcgtgc gtaaaggcgg ggtgattgtt tatccaaccg attccggcta
    34681 tgcgctcggc tgtaaaattg aagataaaaa cgcgatggag cgtatttgtc gtattcgcca
    34741 gctgccggac ggtcacaact ttaccctgat gtgtcgcgat ctttctgaac tctcgaccta
    34801 ttcatttgtc gataacgttg cgtttcgttt aatgaaaaac aacacgccgg gcaactatac
    34861 cttcatcctg aaggggacga aggaggtgcc acgccgtctg ttgcaggaaa aacgcaaaac
    34921 catcggtatg cgtgtgcctt ctaacccgat tgcgcaggcg ttgcttgaag cactgggcga
    34981 accgatgctt tccacttcgc taatgctgcc aggcagcgaa tttaccgaat ccgatcccga
    35041 agaaattaaa gaccgtctgg aaaaacaggt agatttgatt attcacggcg gctatctcgg
    35101 gcagaaaccg acaacggtta ttgatcttac cgatgatacg ccggtcgtgg tgcgtgaagg
    35161 cgtaggtgat gtgaagcctt tcttataaca aattgcgttc cacggatgga agactatggc
    35221 agggaagttt cgctgcattt tgctattgat agttgggctt tttttctctt cgttaagtta
    35281 tgcgaaaaac acggagatgc cttcttatga agaagggatc tcgctctttg atgttgaagc
    35341 cactctgcaa ccggatgggg tgctcgacat caaagagaac attcattttc aggcgcgaaa
    35401 tcagcagatt aagcacggat tttatcgtga tttaccacga ctatggatgc agcctgatgg
    35461 ggacgctgca ctgctgaact atcatattgt tggcgtcacc cgtgatggta ttcctgaacc
    35521 ctggcatctt gactggcata tcgggttaat gagtattgtc gtgggcgata aacaacgttt
    35581 cttgcctcaa ggcgactatc attatcaaat tcattatcag gttaaaaatg ctttcctgcg
    35641 tgagggggat tctgatctgc taatttggaa cgtgaccggt aaccactggc cgtttgaaat
    35701 ttataagacc cgtttttctc tccagttctc taatattgcg ggtaatccat ttagcgaaat
    35761 cgatctcttt accggagaag agggcgacac atatcgtaat ggccgcatcc ttgaggacgg
    35821 aagaattgaa tcccgcgatc cgttttatcg tgaagatttc acggtactct accgttggcc
    35881 tcactcgtta cttagcaatg ccccggctcc acaaacgacg aatattttca gccatcttct
    35941 tttaccctcc acgtcatcgt tgttaatttg gtttccgtgt ctattcctgg tttgtggatg
    36001 gttatatctc tggaagcgca ggccgcaatt tacgccggta gatgtgattg aaaccgatgt
    36061 cattccgcca gattacacgc tcggcatgtt acgtctcgat gcgaagctgg tttacgacga
    36121 taaaggtttt tgtgccgata tcgtaaatct gatagtgaaa ggaaaaattc atctggaaga
    36181 tcagtctgac aagaaccagc aaaccctgat tcgtgttaat gaaggcgtga ccagaaataa
    36241 tgcggtatta ctgcccgcag agcagttatt actggaagcg ttatttcgta aaggcgataa
    36301 ggtcgttctt acggggagac gcaacagagt cttacgcagg gcatttttac ggatgcagaa
    36361 attttatctg ccgcgtaaaa agtcttcgtt ttaccggcct gatacgtttt tgcaatgggg
    36421 tggactggca atattggcgg tcattctcta cggtaacctg agtcctgtag gctgggcagg
    36481 aatgagtctg gtgggcgata tgtttattat gatctgctgg cttcttcctt ttttattttg
    36541 ttcccttgag cttttgttta cccgcgatga tgacaagcct tgcgttaatc gtgtaatcat
    36601 cactttgttt ttaccgctga tttgttcagg cgtggccttt tattctctct atatcaatgt
    36661 cggagatgta ttcttttact ggtatatgcc agcggggtat tttagcgctg ttttcctgac
    36721 cggttatctc actggtatgg ggtatatttt tctgccaaag tttacccaaa ctgggcagca
    36781 acgttatgcc cacggtgaag ctatcgttaa ctatcttgcg cgtaaagagg cggcaacaca
    36841 cagtgggcgg cggcggaaag gggaaacacg gaaactggat tacgcgttgc taggttgggc
    36901 agtctcagca aaccttggaa gagaatgggt tgcacgtatc accccatcac tcacagcggc
    36961 tgttagcgcc ccggaaattg cccgtagtgg cgttttgttc tcattacaga tgcacctgag
    37021 tctgggggcc aataccagtt tgttggggcg aagttattcc ggtggtggt
//
LOCUS       DAZFKF010000043        34675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFKF010000043 DAZFKF010000000
VERSION     DAZFKF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.043"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..640
                     /locus_tag="ACHKA1_002567"
     CDS             <1..640
                     /locus_tag="ACHKA1_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFO5680203.1"
                     /translation="FKNEINKEDILKLMAAREKVAGASDKWTKASGLYSAIVKGHTEI
                     VAAWMETAEVIASHYENDKDVVRELLSLSRNNAVCSLYVASYKTMSKQVIDVYLNAAI
                     RLALQHGFTFDEIVEQFTRDFDGKSFSHVVEKADDIYMGLWLKIFKIVVGENENYLKD
                     VMMQLEAKNNEGKSVISQANGNPVFKELFWKAIDEFNFPQEELNRLNQYRSL"
     gene            complement(779..2143)
                     /gene="mnmE"
                     /locus_tag="ACHKA1_002568"
     CDS             complement(779..2143)
                     /gene="mnmE"
                     /locus_tag="ACHKA1_002568"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO5680204.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGISEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(2249..3895)
                     /gene="yidC"
                     /locus_tag="ACHKA1_002569"
     CDS             complement(2249..3895)
                     /gene="yidC"
                     /locus_tag="ACHKA1_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO5680205.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3898..4155)
                     /gene="yidD"
                     /locus_tag="ACHKA1_002570"
     CDS             complement(3898..4155)
                     /gene="yidD"
                     /locus_tag="ACHKA1_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO5680206.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(4119..4478)
                     /gene="rnpA"
                     /locus_tag="ACHKA1_002571"
     CDS             complement(4119..4478)
                     /gene="rnpA"
                     /locus_tag="ACHKA1_002571"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO5680207.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(4495..4635)
                     /gene="rpmH"
                     /locus_tag="ACHKA1_002572"
     CDS             complement(4495..4635)
                     /gene="rpmH"
                     /locus_tag="ACHKA1_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO5680208.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4865..4945)
                     /locus_tag="ACHKA1_002573"
     CDS             complement(4865..4945)
                     /locus_tag="ACHKA1_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680209.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            5242..6645
                     /gene="dnaA"
                     /locus_tag="ACHKA1_002574"
     CDS             5242..6645
                     /gene="dnaA"
                     /locus_tag="ACHKA1_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO5680210.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6650..7750
                     /gene="dnaN"
                     /locus_tag="ACHKA1_002575"
     CDS             6650..7750
                     /gene="dnaN"
                     /locus_tag="ACHKA1_002575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO5680211.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7750..8823
                     /gene="recF"
                     /locus_tag="ACHKA1_002576"
     CDS             7750..8823
                     /gene="recF"
                     /locus_tag="ACHKA1_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO5680212.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8852..11266
                     /gene="gyrB"
                     /locus_tag="ACHKA1_002577"
     CDS             8852..11266
                     /gene="gyrB"
                     /locus_tag="ACHKA1_002577"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO5680213.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(11290..11388)
                     /locus_tag="ACHKA1_002578"
                     /pseudo
     CDS             complement(11290..11388)
                     /locus_tag="ACHKA1_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11506..11904
                     /gene="yidB"
                     /locus_tag="ACHKA1_002579"
     CDS             11506..11904
                     /gene="yidB"
                     /locus_tag="ACHKA1_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO5680214.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGDVAPQANNDLLSAGMELLKGKLFR"
     gene            12019..12831
                     /gene="yidA"
                     /locus_tag="ACHKA1_002580"
     CDS             12019..12831
                     /gene="yidA"
                     /locus_tag="ACHKA1_002580"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO5680215.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12877..13533)
                     /gene="yidX"
                     /locus_tag="ACHKA1_002581"
     CDS             complement(12877..13533)
                     /gene="yidX"
                     /locus_tag="ACHKA1_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO5680216.1"
                     /translation="MKLNFKGFFKAASLFPLALMLSGCISYALVSHTAKGTSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13669..13782
                     /locus_tag="ACHKA1_002582"
                     /pseudo
     CDS             13669..13782
                     /locus_tag="ACHKA1_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13779..14843)
                     /gene="cbrA"
                     /locus_tag="ACHKA1_002583"
     CDS             complement(13779..14843)
                     /gene="cbrA"
                     /locus_tag="ACHKA1_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO5680217.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDNKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFATLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFICGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKINAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            14944..16158
                     /gene="yidR"
                     /locus_tag="ACHKA1_002584"
     CDS             14944..16158
                     /gene="yidR"
                     /locus_tag="ACHKA1_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO5680218.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGEMVSFTYNDHVMHERSPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCALVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPKDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAESGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(16160..16492)
                     /gene="yidQ"
                     /locus_tag="ACHKA1_002585"
     CDS             complement(16160..16492)
                     /gene="yidQ"
                     /locus_tag="ACHKA1_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO5680219.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASASM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    16732..16804
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            16798..17211
                     /gene="ibpA"
                     /locus_tag="ACHKA1_002586"
     CDS             16798..17211
                     /gene="ibpA"
                     /locus_tag="ACHKA1_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO5680220.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            17323..17751
                     /gene="ibpB"
                     /locus_tag="ACHKA1_002587"
     CDS             17323..17751
                     /gene="ibpB"
                     /locus_tag="ACHKA1_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO5680221.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            17947..19608
                     /gene="yidE"
                     /locus_tag="ACHKA1_002588"
     CDS             17947..19608
                     /gene="yidE"
                     /locus_tag="ACHKA1_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO5680222.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIRTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(19605..20321)
                     /gene="yidP"
                     /locus_tag="ACHKA1_002589"
     CDS             complement(19605..20321)
                     /gene="yidP"
                     /locus_tag="ACHKA1_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5680223.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPDKS"
     gene            20617..22233
                     /locus_tag="ACHKA1_002590"
     CDS             20617..22233
                     /locus_tag="ACHKA1_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO5680224.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            22233..23555
                     /locus_tag="ACHKA1_002591"
     CDS             22233..23555
                     /locus_tag="ACHKA1_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFO5680225.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(23552..24445)
                     /gene="yidL"
                     /locus_tag="ACHKA1_002592"
     CDS             complement(23552..24445)
                     /gene="yidL"
                     /locus_tag="ACHKA1_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5680226.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            24612..26327
                     /gene="yidK"
                     /locus_tag="ACHKA1_002593"
     CDS             24612..26327
                     /gene="yidK"
                     /locus_tag="ACHKA1_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO5680227.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            26324..27817
                     /gene="yidJ"
                     /locus_tag="ACHKA1_002594"
     CDS             26324..27817
                     /gene="yidJ"
                     /locus_tag="ACHKA1_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5680228.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARTEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYQLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVNTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDARPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEDKKQKF"
     gene            complement(27864..28313)
                     /gene="yidI"
                     /locus_tag="ACHKA1_002595"
     CDS             complement(27864..28313)
                     /gene="yidI"
                     /locus_tag="ACHKA1_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO5680229.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            28422..28769
                     /gene="yidH"
                     /locus_tag="ACHKA1_002596"
     CDS             28422..28769
                     /gene="yidH"
                     /locus_tag="ACHKA1_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO5680230.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            28759..29121
                     /gene="yidG"
                     /locus_tag="ACHKA1_002597"
     CDS             28759..29121
                     /gene="yidG"
                     /locus_tag="ACHKA1_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO5680231.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            29118..29615
                     /gene="yidF"
                     /locus_tag="ACHKA1_002598"
     CDS             29118..29615
                     /gene="yidF"
                     /locus_tag="ACHKA1_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO5680232.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDTLQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     HPRRDCQACDWHRLCPHAREETPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(29623..30807)
                     /gene="emrD"
                     /locus_tag="ACHKA1_002599"
     CDS             complement(29623..30807)
                     /gene="emrD"
                     /locus_tag="ACHKA1_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO5680233.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNGGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            30889..30963
                     /locus_tag="ACHKA1_002600"
     CDS             30889..30963
                     /locus_tag="ACHKA1_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680234.1"
                     /translation="MNVSQICARNGEKKNGRICKQKRQ"
     gene            complement(31087..31176)
                     /gene="tisB"
                     /locus_tag="ACHKA1_002601"
     CDS             complement(31087..31176)
                     /gene="tisB"
                     /locus_tag="ACHKA1_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO5680235.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            31741..31839
                     /gene="ivbL"
                     /locus_tag="ACHKA1_002602"
     CDS             31741..31839
                     /gene="ivbL"
                     /locus_tag="ACHKA1_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO5680236.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            31945..33633
                     /gene="ilvB"
                     /locus_tag="ACHKA1_002603"
     CDS             31945..33633
                     /gene="ilvB"
                     /locus_tag="ACHKA1_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO5680237.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPVRVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            33637..33927
                     /gene="ilvN"
                     /locus_tag="ACHKA1_002604"
     CDS             33637..33927
                     /gene="ilvN"
                     /locus_tag="ACHKA1_002604"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO5680238.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            34190..>34675
                     /locus_tag="ACHKA1_002605"
     CDS             34190..>34675
                     /locus_tag="ACHKA1_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680239.1"
                     /translation="MASPINSGIMMSNLCSSTFLDKNINVAAELDITNSEKKYSPGSN
                     FAKWMLQEIKRLIANIMSGSRSVNTEILNYFHPMPGTENNGNRTWMAATGENEYTVIE
                     QTGGKSFKVIQFDAGEKNTKKIVKETSYSGVAVATIIKSLSEKTAALETHSADTVLRK
                     KL"
BASE COUNT         8391 a         8762 c         9165 g         8357 t
ORIGIN      
        1 gtttaaaaat gaaataaata aagaagatat attgaaactg atggcagcaa gagaaaaagt
       61 ggcgggagca tcagacaaat ggacgaaggc atcaggctta tattctgcga tagtgaaagg
      121 gcatacggag attgttgctg cctggatgga gacagctgaa gtgatagcca gccattatga
      181 aaatgataaa gatgtagtga gagaactact gtcgctgagc agaaataatg cagtttgctc
      241 tttgtatgtt gccagctata agacaatgag taagcaggtc attgatgtat atctgaatgc
      301 ggcgattcgc ctggcgttgc aacacgggtt cactttcgat gaaattgtgg agcagtttac
      361 ccgtgacttt gatgggaagt cattctctca tgtggtagag aaagcggatg atatatatat
      421 gggactctgg ctgaaaatat tcaaaattgt ggttggtgaa aatgaaaatt atcttaaaga
      481 tgtgatgatg caactggaag cgaagaataa tgaaggtaaa tctgtaataa gtcaggcaaa
      541 tggcaatcct gtatttaaag aactattctg gaaagcaata gacgaattta actttccgca
      601 agaggagctt aatcggttaa accaatatcg ttctctgtaa agacaggact gcaagatgtt
      661 gaaagtcgct ccagccagta gtaaaggcag ttttggtgca ggatgctcat aaaaaaacaa
      721 gcgctgttcc gttctgatag tagaacggaa cagtgccctt tatatcaatt aagacgactt
      781 acttaccaat acagaagctg gagaaaatcc gccccagcag gtcgtcagag gtaaattccc
      841 cggtgatttc gcttaagttc tgctgcgcca ggcgcaactc ttccgccagt agttcgcctg
      901 cccaggcacc taacagttgc gctttacctt gctgcaaatg ctccgctgcc tgttccagcg
      961 cctgtaggtg gcgacggcgc gccaggaagc cgccttccat gttggtgtca aagcccatgc
     1021 tctgtttgag atggttacgc agcacgtcca cgccttcgcc agtccttgca gacagacgaa
     1081 ttaacgcgtg accgttcact tcacttattc ccagcgtttc gccggtgata tcggctttat
     1141 tgcgcaccac ggtgatcggc agtttcgctg gcagacgggc gataaattcc ggccagatct
     1201 ctgccggatc aacggcgtct gtcgtggtgc catcgaccat aaacagcacg cggtcggcct
     1261 gttcaatttc ctgccacgcg cgctcgatcc caatacgttc aacttcgtca ctggcttcac
     1321 gtagcccggc ggtatcgatg atatgcagcg gcattccgtc aatatggata tgctcacgca
     1381 gcacgtcacg cgtggttccg gcgatatcgg ttacgattgc cgcttcacgg cccgccagcg
     1441 cgtttaacag gctcgattta ccggcgttag gacgtccggc aatcaccacc ttcatccctt
     1501 cgcgcaacaa actaccctga cgtgcttcag cacgcactgc atcgagatcg gcaatcacgt
     1561 cattaagctg ggcttcaatt tttccgtcgg agaggaaatc gatctcttca tcggggaaat
     1621 caattgccgc ttcgacgtaa atgcgcaagt gggtgagggc ttccaccaga tggttgaccc
     1681 gtgcggagaa tgcgccttgc agcgagttaa gtgccgaacg ggctgcctgt tccgaactgg
     1741 catcgataag atcggcaatc gcctcggcct gggctaagtc gagtttatcg ttaagaaacg
     1801 cgcgttcgga aaactcacca gggcgagcaa tccgcaggcc gggaatggtc agaatgcgtt
     1861 ttaacagcag gtcgaggatc accggaccgc catgaccctg cagttccagc acatcttcgc
     1921 cggtgaacga gttcgggcca gggaaccata gcgcaatccc ctgatcgagc acgctgccgt
     1981 cggcgtcttt aaacggaaga taatcggcgt agcgcggctt aggcagttta cccagcacgg
     2041 tttcggcaac ttcacgggct ttgaagccgg agatgcgcag gatgccaacg ccgccacgtc
     2101 ccggaggcgt ggcctgggct acgatagtgt cattatcgct catgatgttc ctgttgcttt
     2161 gtgtggcgga tgcgcggtgc ttatccgccc tacgaaaaga aaaaaggcgg tcaactgacc
     2221 gcccttattt tagcgaaaac tcaccgaatc aggatttttt cttctcgcgg ctatgcaggc
     2281 cacgtttttc cagaccacgg taaatcagct gctgctgaat aatggttacc aggttgctga
     2341 cgatatagta cagcaccaga cctgacggga accacaggaa gaacacggtg aagatgaccg
     2401 gcataaaggt catgatcttc tgctgcatcg ggtcggtcac agtggtcggc gacatcttct
     2461 gaatgaagaa catcgttacg cccatcagga tcggcaggat gtagtacggg tcctgtgcag
     2521 acaggtcgtg gatccacagt gcgaacggcg cctgacgcag ttcaacggaa cccatcagca
     2581 tgtagtacaa cgccaggaag attggcatct ggatcagcag cgggaagcag ccgcccagcg
     2641 ggttaacctt ctcagctttg tacagcgcca tcatttcctg gctgatacgc tgtttgtcat
     2701 cgcccagacg ctcacgcatc gcctgaatct tcggctgcag catacgcatc ttcgccatgg
     2761 aggtgtactg cgctttggtc agcgggtaca tgatgccacg aacgataaag gtgatgatga
     2821 taatggagaa gccccagtta cccacaaagc tatggatcca tttcagcagt ttgaacagcg
     2881 gctgagagat gaaccacaac caaccgtaat caacggtcag atccaggtgc ggagcaacag
     2941 ctgccatttt gtcctggatt tccgggccaa cccacagggt gctgttcatc gcgccagtct
     3001 gaccaggctg aaccagtacc ggctgagatt tatagccgat agcggcgatg ccgttaccca
     3061 gattagcagt atagaagttg ttggtaccgt cgttatgcgg gatccacgcc gtcgcgaaat
     3121 actgttgcag cattgccacc caaccacctt tcgaagagat gttcaggttt tcgttatcgg
     3181 caatggtatc gaacttgtat ttctcatact tctcgtcagg cgtggagtac gccgcgccac
     3241 ggaaggtgtg cagtgcgaag ttgctgcttc cggtatcgag atgcggtggc agagtgatgg
     3301 attgcttcaa ctgaccaaat gaggagattt ccagcggttt ctcgccagca ttctgcacgt
     3361 tgtagttgac gttgacagcg taatcaccac gttttaggac aaacgttttg gtaaacgtgt
     3421 tgcctgccgc gtcggtatac gtcatcggca cctgcagttc gttttgacct tcagccagca
     3481 cataagcgtc tttttcaacg ttatacagcg gacgcgggcc gttagccggg ttatccgggc
     3541 catcacgacc ggtcagaccg ctctgtgcct gataaataaa ctgcggtgaa gtttccagca
     3601 gctggaacgg ctgggtagag ttcagctctt tcgggtaagc aggcagcaga gcttgctcaa
     3661 catcaccacc acgggtgttg atggtcagat caagcacgtc ggtcttaacc gagatcagtt
     3721 tcccctggcc actggccggt acgccctggt cggcggcgct acccgctgcg gtggtcgttg
     3781 tctgcgtggt ctgttgggcc tgaggttgcg ggtttttatc ctgctcccag gcttgccaga
     3841 tcatgaaaga cacgaacagc aaagcgatga ctaaaagatt gcgttgcgaa tccatcgtta
     3901 gtgttctctg gtatcaaatg gtccgggcgg gacgggatcg tcaccaccag ggtgtaaagg
     3961 gtggcatttt aatacgcgtt tcaccgtcaa ccaactgcct tttatcactc caaacctgcg
     4021 caatgcctca attccgtagc ttgaacaggt tggagtgaaa cgacaatgcg gcccgagtag
     4081 cggactaatc aggcgttgat agacccgaat gagggctatc aggacccgcg agccaggcga
     4141 cagtggcggc gccataattt ttccaacgct tccgagagag cacggttatc gaggtcggca
     4201 accccttttt tcgccaccac cacgaaatcc atagccggga gttcatgttg gcgcagacgg
     4261 aagctttcac gcgtcagacg tttaatccga ttgcgttcat gggcgcgtcg aacgtttttc
     4321 ttggcgactg taagaccgat acggggatgc cccagcgaat tcaggcggcc gagaatggta
     4381 atttgcggcg tgccagcccg ttgtggctgc tggaagacga atgtgaattg actgggagtt
     4441 aacaagcgta actccctggg aaatgcgagc ttaaccactc aggggttagc tttattactt
     4501 agaaacggtc agacgagcgc ggcctttagc acgacgacgt gccagaacct gacgaccatt
     4561 tttagtagcc atacgagcac ggaagccgtg agaacggttg cgcttcagta cagacggttg
     4621 aaaagtgcgt ttcatggcga tttctaccta aacttgaata aattcaatgg ctttattgga
     4681 tatccgccga aaaatgaaac gatggacacc gaagccatgg gtgattaaag aggccggatt
     4741 gtaataattg tacactccgg agtcaattct ctttccttat ttaccgcgct tttccgcacc
     4801 ttttcgcagg gaaaacgtac ggcctcacac cagtgaaaac cagcatggcg cgccgggtgg
     4861 aggattatac gggctgacgg gtaaagcgca aggatcgtcc tggatcttta ttagatcgat
     4921 taagccaatt tttgtctatg gtcattaaat tttccaatat gcggcgtaaa tcgtgcccgc
     4981 ctcgcggcag gatcgtttac acttagcgag ttctggatca tcctgtggat aaatcgggaa
     5041 aatctgtgag aaacagaaga tctcttgcgc agtttaggct atgatccgcg gtcccgatcg
     5101 ttttgcagga tcttgatcgg gcatataacc gcagacagcg gttcgtgcgt caccctcaag
     5161 cagggtcttt tcgacgtacg tcaacaatca tgaatgtttc agccttagtc attatcgact
     5221 tttgttcgag tggagtccgc cgtgtcactt tcgctttggc agcagtgtct tgcccgattg
     5281 caggatgagt taccagccac agaattcagt atgtggatac gcccattgca ggcggaactg
     5341 agcgataaca cgctggccct gtacgcgcca aaccgttttg tcctcgattg ggtacgggac
     5401 aagtacctta ataatatcaa tggactgcta accagtttct gcggagcgga tgccccacag
     5461 ctgcgttttg aagtcggcac caaaccggtg acgcaaacgc cacaagcggc agtgacgagc
     5521 aacgtcgcgg cccctgcaca ggtggcgcaa acgcagccgc aacgtgctgc gccttctacg
     5581 cgctcaggtt gggataacgt cccggccccg gcagaaccga cctatcgttc taacgtaaac
     5641 gtcaaacaca cgtttgataa cttcgttgaa ggtaaatcta accaactggc gcgcgcggcg
     5701 gctcgccagg tggcagataa ccctggcggt gcctataacc cgttgttcct ttatggcggc
     5761 acgggtctgg gtaaaactca cctgctgcat gcggtgggta acggcattat ggcgcgcaag
     5821 ccgaatgcca aagtggttta tatgcactcc gagcgctttg ttcaggacat ggttaaagcc
     5881 ctgcaaaaca acgcgatcga agagtttaaa cgctactacc gttccgtaga tgcactgctg
     5941 atcgacgata ttcagttttt tgctaataaa gaacgatctc aggaagagtt tttccacacc
     6001 ttcaacgccc tgctggaagg taatcaacag atcattctca cctcggatcg ctatccgaaa
     6061 gagatcaacg gcgttgagga tcgtttgaaa tcccgcttcg gctgggggct gactgtggcg
     6121 atcgaaccgc cagagttaga aacccgcgtg gcgatcctga tgaaaaaggc cgacgaaaat
     6181 gacattcgtt tgccaggcga agtggcgttc tttatcgcca agcgtctacg atctaacgta
     6241 cgtgagctgg aaggcgcgct gaaccgcgtc attgccaacg ccaactttac cggacgggcg
     6301 atcactatcg acttcgtgcg tgaggcgctg cgcgacttgc tggcattgca ggaaaaactg
     6361 gtcaccatcg acaatattca gaagacggtg gcggagtact acaagatcaa agtcgcggat
     6421 ctcctttcca agcgtcgatc ccgctcggtg gctcgtccgc gtcagatggc gatggcgctg
     6481 gcgaaagagc tgactaacca cagtctgcca gagattggcg atgcgtttgg tggtcgtgac
     6541 cacactacgg tgcttcatgc ctgccggaag atcgagcagc tgcgtgaaga gagccacgat
     6601 atcaaagaag atttttcaaa tttaatcaga acattgtcat cgtaaaccta tgaaatttac
     6661 cgtagaacgt gagcatttat taaaaccgct acaacaggtg agcggtccgt taggtggtcg
     6721 tcctacgcta ccgattctcg gtaatctgct gttacaggtt gctgacggta cgttgtcgct
     6781 gaccggtact gatctcgaga tggaaatggt ggcacgcgtt gcgctggttc agccacacga
     6841 gccaggagcg acgaccgttc cggcgcgcaa attctttgat atctgccgtg gtctgcctga
     6901 aggcgcggaa attgccgtgc agctggaagg tgagcggatg ctggtgcgct ccgggcgtag
     6961 ccgtttttcg ctgtctaccc tgccagcggc ggatttcccg aacctcgatg actggcagag
     7021 tgaagtcgaa tttaccctgc cgcaggcgac gatgaagcgt ctgattgaag cgacccagtt
     7081 ttctatggcg catcaggacg ttcgctatta cttaaacggt atgctgtttg aaaccgaagg
     7141 tgaagaactg cgcaccgtgg cgaccgacgg ccaccgtctg gcggtctgtt caatgccaat
     7201 tggtcaatct ttgccaagcc attcggtgat cgtgccgcgt aaaggcgtga ttgaactgat
     7261 gcgtatgctc gacggcggcg ataatccgct gcgtgtgcag attggcagca acaatattcg
     7321 cgcccacgtt ggcgacttta tcttcacctc taaactggtg gatggtcgct tcccggatta
     7381 ccgccgcgtt ctgccgaaga atccggacaa acatctggaa gcaggctgcg atctcctcaa
     7441 gcaggcgttt gctcgcgcgg caattctctc taacgagaaa ttccgcggcg tgcgtctgta
     7501 tgtcagcgaa aaccagctga aaatcaccgc caataacccg gaacaggaag aagcggaaga
     7561 gatcctcgac gttacctata gcggtgcgga gatggaaatc ggcttcaacg tcagctatgt
     7621 gctggatgtc ctgaacgcgc tgaaatgtga aaacgtccgc atgatgctga ccgattcggt
     7681 ttccagtgtg cagattgaag atgccgcatc acagtcggct gcctatgttg tcatgccaat
     7741 gagactgtaa tgtccctcac ccgcttgttg atccgcgatt tccgcaacat tgaaaccgcg
     7801 gatctcgcct tatctcccgg ctttaacttt ctggtaggtg ccaacggcag tggcaaaacc
     7861 agcgtgctgg aagccatcta tacgctcggc catggtcggg cgtttcgcag tttgcagatt
     7921 ggtcgcgtca ttcgccatga gcaggaggcg tttgttctcc acgggcgatt acagggcgaa
     7981 gagcgcgaga cggcgattgg cttaaccaaa gacaaacagg gcgacagcaa agtccgcatc
     8041 gacggtactg acgggcataa agtcgcggaa ctggcgcacc tgatgccaat gcagctgata
     8101 acgccagaag ggtttacttt actcaacggc ggccccaaat acagaagagc attcctcgac
     8161 tggggatgct ttcacaacga acccggattt ttcaccgcct ggagcaatct caagcgattg
     8221 ctcaagcagc gcaatgcggc gctgcgccag gtgacacgtt acgaacagct acgcccgtgg
     8281 gataaagaac tgatcccgct ggcagagcaa atcagcacct ggcgcgcgga gtatagcgcc
     8341 ggtatcgcgg ccgatatggc tgatacctgt aagcaatttc tccctgagtt ttctctgact
     8401 ttctctttcc agcgcggctg ggagaaagag acagaatatg ctgaggtgct ggaacgtaat
     8461 tttgaacgcg atcgccagct aacctacacc gcgcatggcc cgcataaagc ggacttacgc
     8521 attcgcgccg acggtgcgcc ggtggaagat accttatcgc gtgggcagct taagctgttg
     8581 atgtgcgcct tacgtctggc gcaaggagag ttcctcaccc gtgaaagcgg gcggcggtgt
     8641 ctctacctga tagatgattt tgcctctgag cttgatgatg agcgtcgcgg gctgcttgcc
     8701 agccgcttaa aagcgacgca atcgcaggtc tttgtcagcg cgatcagtgc tgaacacgtt
     8761 atagacatgt cggacgaaaa ttcgaagatg tttaccgtgg aaaagggtaa aataacggat
     8821 taacccaagt ataaatgagc gagaaacgtt gatgtcgaat tcttatgact cctccagtat
     8881 caaagtcctg aaagggctgg atgcggtgcg taagcgcccg ggtatgtata tcggcgacac
     8941 ggatgacggc accggtctgc accacatggt attcgaggtg gtagataacg ctatcgacga
     9001 agcgctcgcg ggtcactgta aagaaattat cgtcaccatt cacgccgata actctgtctc
     9061 cgtacaggat gatgggcgcg gcattccgac cggtattcac ccggaagagg gcgtatcggc
     9121 ggcggaagtg atcatgaccg ttctgcacgc aggcggtaaa tttgacgata actcctataa
     9181 agtgtccggc ggtctgcacg gcgttggtgt ttcggtagta aacgccctgt cgcaaaaact
     9241 ggagctggtt attcagcgcg agggtaaaat tcaccgtcag atctacgaac acggtgtacc
     9301 gcaggctccg ctggcggtta ccggcgagac tgaaaaaacc ggcaccatgg tgcgtttctg
     9361 gcccagcctc gaaaccttca ccaatgtgac cgagttcgaa tatgaaattc tggcgaaacg
     9421 tctgcgtgag ttgtcgttcc tcaactccgg cgtttccatt cgtctgcgcg acaagcgtga
     9481 cggcaaagaa gaccacttcc actatgaagg cggcatcaag gcgttcgttg aatatctgaa
     9541 caagaacaaa acgccgatcc acccgaatat cttttacttc tccaccgaaa aagacggtat
     9601 tggcgtcgaa gtggcgttgc agtggaacga tggcttccag gaaaacatct actgctttac
     9661 caacaacatt ccgcagcgtg acggcggtac tcacctggca ggcttccgtg cggcgatgac
     9721 ccgtaccctg aatgcctaca tggacaaaga aggctacagc aaaaaagcca aagtcagcgc
     9781 caccggtgac gatgcgcgtg aaggcctgat tgcggtcgtt tccgtaaaag tgccggaccc
     9841 gaaattctcc tcccagacca aagacaaact ggtttcttct gaggtgaaat cggcggttga
     9901 acagcagatg aacgaactgc tggcggaata cctgctggaa aacccaactg atgcaaaaat
     9961 cgtggttggc aaaattatcg atgctgcccg tgcccgtgaa gctgcgcgtc gcgcgcgtga
    10021 aatgacccgc cgtaaaggtg cgctcgactt agcgggcctg ccgggcaaac tggcagactg
    10081 ccaggagcgc gatccggcgc tttccgaact gtaccttgtg gaaggggact ccgcgggcgg
    10141 ctctgcgaag caggggcgta accgcaagaa ccaggcgatc ctgccgctga aaggtaaaat
    10201 ccttaacgtt gagaaggcgc gcttcgacaa gatgctctct tctcaggaag tggcgacgct
    10261 tatcaccgcg cttggttgtg gtatcggtcg tgacgagtac aacccggaca aactgcgtta
    10321 tcacagcatc atcatcatga ccgatgcgga cgtcgacggc tcgcacattc gtacgctgct
    10381 gttgaccttc ttctatcgtc agatgccgga gatcgttgaa cgcggtcacg tctacatcgc
    10441 tcagccgccg ctgtacaaag tgaagaaagg caagcaggaa cagtacatta aagacgacga
    10501 agcgatggat cagtaccaga tctctatcgc gctggacggc gcaacgctgc acaccaacgc
    10561 cagcgcaccg gcattggctg gcgaagcgtt agagaaactg gtgtctgagt acaacgcgac
    10621 gcagaaaatg atcaaccgca tggagcgtcg ttatccgaaa gcaatgctga aagagcttat
    10681 ctatcagccg acgctgacgg aagccgacct ctctgatgag cagaccgtta cccgctgggt
    10741 gaacgcgctg gtcagcgaac tgaacgacaa agaacagcac ggcagccagt ggaagtttga
    10801 tgtccacacc aatgccgaac aaaacctgtt tgaaccgatt gttcgcgtgc gtacccacgg
    10861 tgtggatact gactatccgc tggatcacga gtttatcacc ggtggcgaat atcgtcgtat
    10921 ctgcacgctg ggtgagaaac tgcgtggctt gctggaagaa gatgcgttta tcgaacgtgg
    10981 cgagcgtcgt cagccggtag ccagctttga gcaggcgctg gactggctgg tgaaagagtc
    11041 tcgtcgcggt ctctccatcc agcgttataa aggtctgggc gagatgaacc cggaacagct
    11101 gtgggaaacc accatggacc cggaaagccg tcgtatgctg cgcgttaccg ttaaagatgc
    11161 gattgctgcc gaccagttgt tcaccacgct gatgggcgac gccgttgaac cgcgccgtgc
    11221 gtttattgaa gagaacgccc tgaaagcggc gaatattgat atttaatggc gttaaccatg
    11281 cgagcgtgcc tgatgcgcta cgcttatcaa gcctacgaga ctcctgcaat atattgattt
    11341 tgcatgtttt tgcaggccgg ataaggcgtt cacgccgcat ccggcaagaa gctataagaa
    11401 aagggcggag atcatctccg ccctttttat ttctgcaatc cgcaattatc atcccgcagt
    11461 tctatacttt ttgggtcaac acgtaacgct ttacaggtga ttatcatggg gctttttgat
    11521 gaagttgtcg gcgcctttct gaaaggcgat gcggggaaat atcaggcgat tttaagttgg
    11581 gttgaggagc agggcggcat tcaggtgctg ctggaaaaac tgcaaagtgg cggcttaggg
    11641 gccattctct caacctggct gagtaatcaa cagggcaatc aatcggtgag cggcgaacaa
    11701 ctggaatcgg cgctcggcac aaatgcggta tccgatctcg ggcaaaaact tggcgtggat
    11761 accagtacgg cttccagttt actggcagaa caattgccga agattattga tgctctctcg
    11821 ccacagggcg atgtagcgcc gcaagccaac aatgatttac tttccgcagg gatggaacta
    11881 ctgaaaggga aactcttccg ctaagcaaat ggggagcatg caacccgcgt tgcatccccc
    11941 ttttccctcg ggtgttgtac tgatttttga gcggaatcgc gttagcatgg gtcaggaacc
    12001 aatctacctg gggaactcat ggctatcaaa ctcattgcta tcgatatgga tggcaccctt
    12061 ctgctgcccg atcacaccat ttcacctgcc gttaaaaatg cgattgccgc agctcgcgcc
    12121 cgtggcgtga atgtcgtgct aacgacgggt cgcccttatg caggtgttca caattacctg
    12181 aaagagctgc atatggaaca gccgggtgac tactgcatta cttataacgg cgcgctggta
    12241 cagaaggccg ctgatggtag caccgtggcg caaactgctc tcagctatga cgactaccgt
    12301 ttcctggaaa aactctctcg cgaagtcggt tctcatttcc acgcactgga ccgcaccacg
    12361 ctgtacaccg ccaaccgtga tatcagctac tacacggtgc atgaatcctt tgttgccact
    12421 attccgctgg tgttctgcga agcggagaaa atggacccca atacccagtt cctgaaagtg
    12481 atgatgattg atgaacctgc catcctcgac caggctatcg cgcgtattcc gcaggaagtg
    12541 aaagagaaat ataccgtgct gaaaagtgcg ccgtacttcc tcgaaatcct cgataaacgc
    12601 gttaacaaag gtacgggtgt gaaatcactg gccgacgtgt taggtattaa accggaagaa
    12661 atcatggcga ttggcgatca ggaaaacgac attgcaatga ttgaatatgc aggcgtcggt
    12721 gtggcgatgg ataacgctat tccttcggtg aaagaagtgg cgaactttgt caccaaatct
    12781 aaccttgaag atggcgtggc gtttgctatt gagaagtatg tgctgaatta atcagtggac
    12841 gggcaaacag cataaacgcg tttgcccgct tactgattat tggtttactg catatacaac
    12901 cagaaattgc agcggtgcag taatgatgtc cagcgtaaca gtcactggca gtaatgctgc
    12961 ggaaacacca tccggaatgc ctctgggtga atagtattca taaaatcgca ctttaaatgg
    13021 atggtagaac gccatcacct ttgagtaatc catgttttta tttttctcat ggattgtgcc
    13081 ttttaggttt tccagtgacc tggtacagtg ttgaacacca caggcaaaac cataatgcgt
    13141 tgccagtgcc ttttcttctt cgttattcca gtagtagtaa agcgatattg tgccggtaaa
    13201 tttctttttc cccgcattta aaacaaatct tgcgtcatca gcaacctgaa tattgtgccg
    13261 gttaagtgct ggatcattga gcatcttaac gatgtcatca gcaccatctg tgataaggta
    13321 atccagcgat tcccctacaa aaacatagcc ttttgttcca ttactatcct ttgcctgcga
    13381 tagccccgtg atggtgtctg actgcgattg atactttcct gaagtaccct ttgcggtatg
    13441 ggaaaccaga gcatacgaga tacagcctga aagcatcagt gctagtggga ataaactggc
    13501 agccttaaaa aatcccttaa aattcaactt catgattaat cctttaaacg agtagctaag
    13561 ccgttgaaag catactcttc tttccctcct gtttagcgcc tgatctccct tttcagattt
    13621 ctgcccgata taagaccttt tcttatgagg tgtttttttg tggactaata tctgttgcaa
    13681 ggctatggct tcgccccggc actggtttat atctccaccg tcgcgttgat tggtgcgctc
    13741 tcttacatcc tgctggtggg cgatgtgaag cgcgttggct aatccttcaa ctgtggaata
    13801 tgtgccacac cactgcgcat aatccacttt cttaaagccg gtgcggtcag gcatcggctt
    13861 tttactatct tgccgaagag ttttaaacgc agtttgcggg tggcgcgcca gtaggctgcg
    13921 ttgatcttct ctggctgctt cagtaacacc gaacgcagaa tctctgcgct atccagcgca
    13981 tagcttatcc cctccagcga gctggcgctg ataaatcccg ccgcttcacc aatcagaaag
    14041 gcgttgtcct taccgcagat aaaatcctgc cagcgtgagg gaaacagcac tgtgcatttt
    14101 tcgcttttca ccgctttgcc aaactggaac tgaaaggcgc tcattttctc tttcagcgtc
    14161 gcgaaacgcg tctgaccgtc tttcattgga taggcaccac caaagataaa ataaccgtct
    14221 ttgctgatac tccatgagta acagtcagtt atcgcattat caaagatgca ggagtagaac
    14281 ggcaccggat gtttctctgc gaaccactgc tggattgcga catatttacg gatttgatga
    14341 tccgggtaga gatgtcgccg caccatcgag ttggcaccat cggcaccgac cagatagcgg
    14401 gcggtaatat gctgctccca gccgtctgca cgaaaaatga catgccattt attatcctca
    14461 cgccagattt tccggcacag gctatcgtgg taaacctcaa cgctggcggg gatcagcgat
    14521 ttcatccaca agtcgaaagc atggcgatta atattgatat agcttcgctg gtagttacgc
    14581 gtgagcgatg cggcgacgtc gacggttttg acgctgaaaa tctgcggatt agcgatcaca
    14641 tcgacaggaa gcgtcagtcc atcgcgaata aaagatcgct gcgcgtccgg tgccagcaga
    14701 ccgccgcagg gtttgctgaa accttcagta ccacactggt gctttttatc cagcgcgatc
    14761 acctgcattt tgcctgctaa ctttcgtgcc aacgccgatc ctgccgggcc gaggccaata
    14821 atcgccacgt cgaaatgttc catcttttca ctcacattca tcacgatggc aaaagactat
    14881 tgtctgaaag tgaagtcctt gtgaagtatg atggcgtgaa ccgttttgta tcaggaccac
    14941 gccatgaaac agataacctt tgctccccgt aatcacctgc tcaccaatac caatacctgg
    15001 acgcccgaca gccagtggct ggtatttgac gtgcgtcctt caggggcgtc gtttaccggc
    15061 gagaccattg agcgtgtgaa tatccatact ggagaggtcg aggttatcta tcgcgcgtca
    15121 cagggcgcac acgtcggcgt ggtgaccgtt catccgaagt cagaaaaata tgtcttcatt
    15181 cacggcccgg aaaatcctga tgaaacatgg tattacgatt tccatcatcg gcgcggagtc
    15241 attgctgaag gcggcaaggt gagcaatctc gatgcaatgg atattactgc accgtacacc
    15301 ccaggagcgc tgcgcggcgg cagccatgtg catgtcttta gcccgaacgg tgaaatggtg
    15361 agctttacct ataacgacca tgtaatgcat gaacgcagcc cggcgctgga tttgcgaaac
    15421 gtcggggttg ctgcaccgtt tggcccggtc aacgtacaaa agcagcatcc gcgtgaatac
    15481 agcggtagcc actggtgcgc actggtgagt aaaacaacgc ccacgccgca gcctggcagc
    15541 gatgaaatca atcgtgctta tgaagaagga tgggtaggaa atcacgcgct ggcgtttatt
    15601 ggcgacacac tttcgccaaa gggcgagaaa gtaccggagc tgtttattgt cgagttgccg
    15661 aaagatgaag ctggctggaa agcagctggt gatgcgccgt taagcggaac ggaaacaacc
    15721 ctgcccgcgc caccgcgtgg cgtcgtgcag cgacgtttaa cctttaccca ccatcgggct
    15781 tatccggggt tagtcaacgt cccgcgccac tgggtgcgct gtaatccgca gggtacacaa
    15841 atcgcgtttt taatgcgaga tgataacggc attgtgcaac tgtggcttat ctcgccacag
    15901 ggcggcgagc cgcgccagtt aacccataac aaaacggata ttcagtctgc atttaactgg
    15961 catccatcag gagaatggtt gggctttgtg ctggataatc gaattgcttg tgcccatgcg
    16021 gaaagcgggg aggttgagta tttaaccgaa aaccacgcca atccaccttc tgcggacgcc
    16081 gtggtcttct cgccggatgg tcaatggctg gcgtggatgg aaggcggtca gctgtggatc
    16141 accgaaactg atcgctaaat cagttatcag gcattctggc cggtggaata accgcattgg
    16201 tggcttgcgc gttggcttcg cttttttcaa cgcgtgagcg caccgagctg tctttgcgga
    16261 acacatccca cggcagtaaa agtgtgtcca tcacagcggt aaacggcata tcgagaatcg
    16321 ccagcgactt agtgccccag ttggtttcat cgtcgccaat catgctggcg ctggcgcggg
    16381 tgccgggata cgttccttct ttccccccgg tatgggacat cacgctggag catcctccga
    16441 gtaatgtcat tccgctgcat atcatgaacg ctaacaacac atttcttatc ataataaatt
    16501 catctgttga tcgtgggtgt tggcctgatg agttatagcg atcccttgct gaaaataaca
    16561 tcatcattac gtcgcactgt ggcggctatc gcactttaac gtttcgtgct gccccctcag
    16621 tctatgcaat agaccataaa ctgcaaaaaa aagtccgctg ataaggcttg aaaagttcat
    16681 ttccagaccc atttttacat cgtagccgat gaggacgcgc ctgatgggtg ttctggctac
    16741 ctgacctgtc cattgtggaa ggtcttacat tctcgctgat ttcaggagct attgattatg
    16801 cgtaactttg atttatcccc gctttaccgt tctgctattg gatttgaccg tttgtttaac
    16861 cacttagaaa acaaccagag ccagagtaat ggcggctacc ctccgtataa cgttgaactg
    16921 gtagacgaaa accattaccg cattgctatc gctgtggctg gttttgctga gagcgaactg
    16981 gaaattaccg cccaggataa tctgctggtg gtgaaaggtg ctcacgccga cgaacaaaaa
    17041 gagcgcacct atctgtacca gggcatcgct gaacgcaact ttgaacgcaa attccagtta
    17101 gctgagaaca ttcatgttcg tggcgctaac ctggtaaatg gtttgctgta tatcgatctc
    17161 gaacgcgtga ttccggaagc gaaaaaaccg cgccgtatcg aaatcaacta attccctaag
    17221 gccgccttgc gcggcctgac atctccatgc tcgccgtcag ggagcatatg cgaatcttcg
    17281 gatttgcagg tacttactcg cttcttagaa ggagaaatga ctatgcgtaa cttcgattta
    17341 tccccactga tgcgtcaatg gatcggtttt gacaaactgg ccaacgcact gcaaaacgcc
    17401 ggtgaaagcc agagcttccc gccgtacaac attgagaaaa gcgacgataa ccactaccgc
    17461 attacccttg cgctggcagg tttccgtcag gaagatttag agattcaact ggaaggtacg
    17521 cgcctgagcg taaaaggcac gccggagcag ccaaaagaag agaaaaaatg gctgcatcaa
    17581 gggcttatga atcagccatt tagcctgagc tttacgctgg ctgaaaatat ggaagtctct
    17641 ggcgcaacct tcgtaaacgg tttactgcat attgatttaa ttcgtaatga gcctgaaccc
    17701 atcgcagcgc agcgtatcgc tatcagcgaa cgtcccgcgt taaatagcta actagctatt
    17761 ctctttgccc cgccattctg gtggggcttt ttttgtcttt cttcttctac aatgcgttaa
    17821 atttcgactg tttaagatat ttcggcacgt tctttcccgt gataatgtgc gctataaggc
    17881 aaatctgctt caccgattca gccataatcc gtagcaatta aaatgttatc tcaagagaaa
    17941 tggacgatga gtgatatagc attaacggtc agtattctgg ctttggtggc agtcgtcggt
    18001 ttgtttatcg gcaacgtcaa atttcgcggc ataggattag gtattggcgg cgtgctgttt
    18061 ggtgggatca tcgtcggcca ttttgtttct caggcgggga tgacattaag tagcgatatg
    18121 ctgcatgtta ttcaggaatt tggtctgatc ctgttcgttt atactatcgg gattcaggta
    18181 gggccgggct tctttgcctc attgcgcgtc tccggattac gcctcaacct gtttgctgtt
    18241 ctgatcgtca tcatcggtgg tctggttacc gccatcctgc ataaactgtt tgatattcca
    18301 ctgccggtag tgctggggat tttctccggt gcggtaacca atacgccagc gctgggggca
    18361 gggcagcaga tcttgcgcga cctgggtaca ccaatggaaa tggtcgatca gatggggatg
    18421 agttacgcga tggcgtatcc attcggcatt tgcgggatat tgttcaccat gtggatgttg
    18481 cgggttattt tccgcgtcaa tgtcgagaca gaagcccagc agcacgaatc ttcacgcacc
    18541 aatggcggcg cgctgatcag gactatcaat attcgcgttg agaaccctaa cctgcatgat
    18601 ttagccatta aagatgtacc gattctcaac ggcgacaaaa ttatctgctc gcgtctgaaa
    18661 cgcgaagaaa ccctaaaagt tccttcgcca gataccatta tccaactggg cgatttgctg
    18721 catctggtgg gtcagccagc ggatttacat aatgcgcaac tggtgattgg tcaggaggtc
    18781 gatacttcgc tgtccacgaa aggcactgat ttgcgcgtcg agcgtgtggt ggtcaccaat
    18841 gaaaacgtgc ttggtaagcg tattcgcgac ctgcatttta aagaacgcta tgacgttgtt
    18901 atctcgcgcc tgaaccgtgc cggggtcgaa ctggtcgcca gtggcgatat cagcctgcag
    18961 ttcggcgata tcctcaacct ggtggggcgt ccgtccgcaa ttgatgccgt tgccaatgtg
    19021 ctggggaatg cgcagcaaaa actgcaacag gttcagatgc tgccagtgtt tattggcatc
    19081 gggcttggcg tattgttagg ttctattccc gtctttgtgc ctgggttccc ggccgcgttg
    19141 aaactggggc tggcgggcgg tccgctgatt atggcgttga tcctcgggcg tatcggcagt
    19201 atcggcaagc tgtactggtt tatgccgcca agtgccaacc tcgcgctgcg ggagctgggg
    19261 atcgtgctgt tcctctcggt cgttggtctg aaatctggtg gggattttgt gaataccttg
    19321 gtcaatggcg aagggctaag ctggattggt tatggtgccc tgatcaccgc cgttccgctg
    19381 attactgttg gcattctggc gcggatgtta gccaaaatga attacctgac catgtgcggg
    19441 atgctggcag gttccatgac cgatcctccg gcgctggcgt ttgctaataa tcttcatcca
    19501 accagcggtg cggcggcgct ctcttacgcc actgtctatc cgttagtgat gttcctgcgc
    19561 attatcaccc cccaattact ggcggtgctc ttctggagta tcggttaact cttgtccggg
    19621 tggagtcgcc gtaaatggta ctccacctgg tattcgctgg catttctgaa catcactgaa
    19681 taattcaaaa actccccgct ctcgctatat gacaacgagg tgatccgcag cagtggcgtg
    19741 tgttccgcta ccttcatttg ccgcgccagc agtctatcgg cgagcaccgg cgtcaggctt
    19801 tcataattac cgccaatcag aatcccgcac tcttgttcaa tatattcaaa cttcgaccct
    19861 tccagatgtt gcagcgaaag attacggaac agttttaccg gcatatagct gtcttccagc
    19921 atcagcggtt tcccttccac aaaacgaacg cgacgggaga agtagatctg ctcgttgatt
    19981 tgaatccgta actggctggc aattgccgga ggcgcaggca tgatttcaaa aatcagcacc
    20041 tggctggtga ctgtttttcc ctgccgtttt aacacctcca ccagcccggt caggctggcg
    20101 gtttgatgca gtacatcttt gcgcaccagg taagtgccgc tgccgtggcg gcggaccacc
    20161 agcccccacg ctaccagcag gtcaatggct ttacggatgg tcatccgcga taccgcaaac
    20221 tcttccgcca gctttttttc accgggaaga aggctgttta gcgtgaaatc tgcggaatta
    20281 agtctaattc ttaaccgctc cgcaatgctt ttgtagatca tctctagatc tcttttttgc
    20341 ctgcaagccc gggtaatgcg cccacaagtt ctgcttaatc gattgaaaaa acatcttttt
    20401 tttaaagatg tgttcgatgc tgtgtacctg tgctcacaga ggtctacttt ttcgcgaaaa
    20461 cgtagatctc taccgcccaa cgaaaagcat gaaagcgatc acgaatccca ttgggtcgtc
    20521 atgttctgcc gatcgcatct tcctatcctc gctccaggcc tgccgcataa ccaatcaggc
    20581 ttcctactta cagaattgag aaaagaggat gtggaaatgc tcagtcaaat tcaacgcttt
    20641 ggcggcgcga tgttcacgcc agtgctgctg tttcccttcg ccgggattgt ggtgggtctt
    20701 gccatcttgc tgcaaaaccc gatgtttgtc ggggaatcac tgaccgatcc gaacagttta
    20761 ttcgcgcaaa tcgtacacat tattgaagag ggcggatgga cggtattccg taatatgccg
    20821 ctgatttttg ctgtcggttt acccattggc cttgctaagc aagcgcaggg gcgtgcttgt
    20881 ctggcggtga tggtgagttt cctgacctgg aactatttca tcaacgcgat gggaatgacc
    20941 tggggaagct acttcggcgt cgatttcact caggacgcgg tggcaggtag cggtctgaca
    21001 atgatggccg ggattaaaac cctcgatacc agcattatcg gcgcaattat catttccggc
    21061 attgtgacgg cgctgcataa ccgtctgttc gataaaaaac tgccggtgtt tctcggcatt
    21121 ttccagggga cgtcttatgt ggtgattatc gccttcctgg tgatgatccc ctgtgcctgg
    21181 ctgacattgc tcggctggcc aaaagtacaa atggggattg aatctctgca agcgttcctg
    21241 cgttcggcgg gtgcgcttgg ggtgtgggtt tacaccttcc tcgaacgtat tctgatccca
    21301 accggtttac accacttcat ctacggaccg tttatctttg gtccggcagc tgttgaaggc
    21361 ggcattcaga tgtactgggc gcagcatctg caagagttca gtttgagcgc cgagccgctg
    21421 aaatcgttgt tcccggaagg cggttttgcc ctgcacggta actcaaaaat ctttggtgcc
    21481 gtgggcattt ctttagcgat gtacttcact gccgcaccgg aaaatcgggt aaaagtggcg
    21541 ggcttgctga ttcccgcaac cttaaccgcc atgctggtgg gaattaccga accgctggaa
    21601 tttaccttcc tgttcatttc accgttgctg tttgcggtac acgctgtgct tgcggcctca
    21661 atgtcgaccg tgatgtatct ctttggtgtg gtgggcaaca tgggcggagg tctgattgac
    21721 caggttttac cgcaaaactg gatcccgatg ttcagcaacc acgcggatat gatgttgacc
    21781 caaatcgcca ttgggttgtg ctttaccctg ctgtacttcg tggttttccg caccctgatt
    21841 ctgcaattca acatgtgcac gccgggacgt gaagatgcgg aagtgaaact ctactcaaaa
    21901 gccgaataca aagcctcgcg aggccaaacc accgcggcag agccaaaaaa agagctggat
    21961 caggctgccg gtatcctgca agccctgggc ggggtcggca atatctccag catcaacaat
    22021 tgcgcgacgc gtctacgtat tgcactgcat gacatgtcac aaacgctgga tgacgaagtc
    22081 tttaaaaagc tgggagcgca cggcgtcttc cgtagtggcg atgccattca ggtgatcatt
    22141 ggtctgcatg tatcccagct gcgtgaacag ctcgatagct taattaattc tcatcaatca
    22201 gcagaaaatg ttgccattac ggaggcagta taatgaccaa attctcagtg gttgtcgcag
    22261 gcggtggaag cacctttacg ccaggcatcg tgttgatgct cctggcgaat caggaccgtt
    22321 tcccgcttcg tgcactgaaa ttttatgata acgatggcgc gcggcaggaa gtgattgccg
    22381 aagcctgtaa agtcatcctt aaagagaaag cgccggacat tgcgtttagt tacaccaccg
    22441 atcctgaagt ggcgttcagc gacgttgatt ttgtcatggc gcacatccgc gtcggcaaat
    22501 acccgatgcg cgaactggat gaaaaaatcc cgctgcgcca cggcgttgtt ggtcaggaaa
    22561 cttgcggacc cggcggaata gcgtacggca tgcgttccat tggcggcgtc ctggaactgg
    22621 tggattatat ggaaaaatat tcaccaaacg cctggatgct caactactcc aacccggcag
    22681 ccattgtcgc agaagccacg cgtcgtctgc gcccgaatgc gaaaatcctc aacatctgtg
    22741 acatgccaat cggtattgaa agccggatgg cgcaaattgt tgggctgcaa gatcgcaaac
    22801 agatgcgcgt gcgctactac ggcctgaacc actttggctg gtggacatca attgaagatt
    22861 tgcagggcaa cgacctgatg ccccagctgc ggcaatatgt ctcgaagcac ggttatgttc
    22921 caccgcagca agatacacat actgaagcga gctggaacga cacctatgca aaagcgcggg
    22981 atgtccaggc actggacccg gatacattac caaacaccta tctgaaatat tatctcttcc
    23041 cggattatgt cgttcagcat tccaaccctg aacatacccg cgcgaatgag gtgatggaac
    23101 atcgcgagaa acaggttttc gatgcttgcc gcgccattac ggcggcagga aattcagcgg
    23161 cgggcaagct ggagattgac gaacatgcgt catacatcgt cgatctggcg gcggcaattg
    23221 ccttcaacac tcaggagcgg atgttgctga ttgtgcctaa caacggggca atccataact
    23281 ttgatgatga agcgatggtc gagatcccgt gtctggtcgg gcacaacgga ccagaaccac
    23341 tggtggtcgg cgatatcccg cagtttcaga aagggttaat gagtcagcaa gtggcggtgg
    23401 aaaaactggt cgtggacgcc tgggaacagc gttcatatca gcacctgtgg caggcgatta
    23461 cgttgtcgaa aacggtaccg agcgcctcgg tcgccaaagc tattctggat gagttgctgg
    23521 aggccaacaa agcgtactgg ccagagttac gttaattgtg tggactggca tccgttgggt
    23581 gctggtccgt attctttagc acggctgaag gcgcataacc aaatttatgt ttaaaggctt
    23641 tgctgaaatg aaaggagtcg aagaagttga gcgtatcagc gacttcgcca accgagtttc
    23701 cttgttgctt taatagcgat agcgccagat ccagacgcgc atcgaggtaa tattctttcg
    23761 gcgtcttgcc ggtatagcgt aaaaacagac gacgcaacca ggcttcgcta caggggatcg
    23821 tggcagccat atcggctacg ctccagcgtt gttgcagact ggcatgtaaa gtggcaatta
    23881 atttttcaat ttgccgccgt tgtggatctt ttttaccgtc tgcgtacata agacaaatcc
    23941 actgataaat aattttcgtc agaaaagcga ctgccagatt attttttatt gcttctggtg
    24001 aagttattaa ctcagcaact tcggtgagtt cctgattata aatttcgcca ttataaataa
    24061 cgctttgctg accaacggga atatccatca tactggtggg ggtaaattcc atccagtact
    24121 gttcccagac taaaccttca cagtgataag agtgaatatc cattggcttt aaaaatataa
    24181 tacagttgcc atgcagagtt atttgttcgc cattctttaa aagaattttc ccacatcctt
    24241 gtacggtata caccgcaacc catgagttgg cgataagcgg tttcttcttg tcccgcggcc
    24301 agatcacccg gtaatgctcg tcggcgtagg tatgccagag cgagatcaga gaaattccgc
    24361 tggagatcac attttcgtta atcaataagg gaatattgta tggagtttcg ttttttacat
    24421 ctgaagtttg caattttcca ttcattttaa tatccctccg gcgaattatg ctcacaacct
    24481 gaacttaaca gaaaacaatc cgttcaggga tacgacgttt gtattttaag aatctgactg
    24541 cctgacccga cgcattttac ctctccctat attcatgcgt ccgggacata aacataaata
    24601 agggctatga gatgaattcg ttacaaatct tgagttttgt cggttttacg ctgctggtgg
    24661 cgatcatcac ctggtggaag gttcgcaaaa cagataccgg atcgcaacaa ggctattttc
    24721 ttgccggacg ttcactaaaa gcgccggtta ttgctgcttc gttaatgtta accaaccttt
    24781 ccacggaaca actggttggt ctttccgggc aggcctacaa aagcggcatg tcggtgatgg
    24841 gctgggaagt gacttcagcg gtgacgctga tcttcctcgc gctaatcttt ttaccgcgct
    24901 atctgaagcg cggcattgcc accatccccg attttctgga ggaacgttat gataaaacga
    24961 cgcgtattat catcgacttc tgcttcctca ttgccaccgg cgtctgcttt ctgccgattg
    25021 ttctctactc cggcgcgttg gcgctcaaca gcctgtttca cgtcggggaa tcgctacaga
    25081 tttctcacgg tgcggctatc tggctactgg taattttgct tggtctggcg ggaattttgt
    25141 atgcggtgat cggcggactg cgcgcaatgg cagtggcgga ctccatcaac ggtattgggc
    25201 tggttatcgg cgggttgatg gtgccggtat ttggcctgat cgcgatgggc aagggcagct
    25261 ttatgcaggg cattgagcaa attaccaccg ttcacgccga gaaattaaac tcagtcggtg
    25321 gcccgaccga tcccttgccg attggcgcgg catttaccgg tttaattctg gtgaacacct
    25381 tttactggtg tacaaatcag ggcatcgtgc aacgcacgct ggcgtcaaaa agcctggcgg
    25441 aagggcaaaa gggggcgctg ttaacggcgg tgctgaaaat gctcgacccg ctggtactgg
    25501 tgctgccagg gttgattgcg tttcatctgt atcaggatct accgaaagcc gacatggcct
    25561 acccgacgct ggtcaataac gttctgccag tgccactggt gggtttcttc ggcgcggtgt
    25621 tatttggtgc ggtgatcagt accttcaacg gctttctgaa tagcgccagt acgttattca
    25681 gtatgggtat ttaccgtcgc atcattaacc agaatgccga gccgcagcag ctggtcaccg
    25741 tcgggcgcaa atttggtttc tttatcgcta tcgtttcggt gctggtagcg ccgtggatcg
    25801 ccaacgcgcc gcaggggctg tatagctgga tgaaacagct caacggcatt tacaacgtgc
    25861 cgctggttac catcatcatt atgggctttt tcttcccgcg catcccggcg ctggcggcaa
    25921 aagtggcgat ggggattggc ataatcagct acatcaccat caactatctg gtgaagttcg
    25981 acttccattt cctctatgtg ctggcctgta cgttctgcat caacgtggtc gtgatgctgg
    26041 tgatcggttt tatcaaaccg cgcgccacgc cgttcacctt caaagatgcg tttgcggtgg
    26101 acatgaaacc gtggaaaaac gtcaagatcg cgtcaattgg catcctgttc gcgatgattg
    26161 gcgtctatgc cgggctggct gaattcggcg gctacggtac gcgctggtta gcgatgatca
    26221 gttatttcat tgccgccgta gtgattgtct acctgatttt tgacagctgg cggcatcgtc
    26281 acgacccagc cgtaaccttt actcccgatg caaaggatag cctatgaaac gccccaattt
    26341 tctgttcatc atgaccgata cccaggccac taatatggtc ggttgctata gcggtaagcc
    26401 gctgaatacg caaaatattg atagtctggc ggcggaaggt attcgcttta actccgccta
    26461 cacctgttca ccggtttgta caccggcgcg cgccgggctg tttaccggta tctacgctaa
    26521 ccagtccggc ccgtggacca acaacgtcgc gccaggcaaa aacatttcca ctatggggcg
    26581 ctactttaag gatgcgggct atcacacttg ttacatcggc aaatggcatc tcgacggtca
    26641 tgactatttc ggcactggcg agtgtccgcc ggagtgggac gctgattact ggttcgatgg
    26701 ggcgaactat cttagcgaac tgacggaaaa agagattagc ctgtggcgca atggcctaaa
    26761 cagcgtcgaa gatttacagg cgaaccatat tgacgaaacc ttcacctggg cgcaccgcat
    26821 cagcaatcgg gcggtggatt ttctgcaaca gcccgcgcgc accgaggaac ccttcctgat
    26881 ggtggtttcg tatgatgagc cgcatcaccc gttcacctgt ccggtggagt atttagagaa
    26941 atacgctgat ttttactacc aactgggcga gaaagcacag gatgacctgg cgaacaaacc
    27001 agaacatcac cgcttatggg cgcaggcgat gccatcgcca gtcggtgatg acgggcttta
    27061 tcaccatccg ctctattttg cctgtaatga ctttgttgat gaccaaatag gacgggtcat
    27121 caatgcctta acgccagagc aacgtgaaaa tacgtgggtt atttatacat ccgatcacgg
    27181 cgaaatgatg ggcgcacata agctgatcag taaaggagcg gcgatgtatg acgacatcac
    27241 ccgtattccg ctgatcatcc gttcgccgca aggggagcgg cgacaggtca atacgccagt
    27301 cagtcatatc gatttactgc cgacaatgat ggcgctggca gatattgaaa aaccagagat
    27361 tctgccgggg gaaaatatcc ttgccgtgaa agagccacgc ggcgtgatgg tggaatttaa
    27421 ccgctacgag attgagcatg acagctttgg cggttttatt ccggtgcgtt gctgggtgac
    27481 ggatgacttt aaactggtgc tcaacctctt caccagtgat gaactttacg atcgccgtaa
    27541 tgacccaaat gaaatgcata acctgatcga tgatgcccat tttgccgacg ttcgcagcaa
    27601 aatgcatgat gccttattgg attatatgga caaaattcgc gatccgttcc gcagctacca
    27661 atggagcctg cgtccgtggc gtaaagatgc acggccgcgc tggatggggg cgtttcgtcc
    27721 acgcccacaa gatggctatt cgccagtggt acgtgactat gacaccggcc tgccgacaca
    27781 aggggtgaaa gtggaggata aaaaacagaa gttctgacga taatgcccgc tgatgatcac
    27841 ccggcgggca ttattcaggc tgcttaaacc agtgaaccgc cggtcaggaa ggaaaagata
    27901 aggaatatca gcaaaatgct gcacacgatg ccgataccaa aaagcaaggt atggaaagcc
    27961 agcgtcccgc cgccaaaaac cagcgcgccg cgaatgcccc agcgattttc cttacgcata
    28021 ccgccaataa atgcgcaaag tagcgcagca atggccagtg cgataccgct ttgatcgagg
    28081 attttatcga catctacgtc ctcttttttc tccacggtgg taattttttc gccttttatt
    28141 ccggcgagaa gacctctttt aatttcagcc gttttgtccg ccacgatgct ttcaagcgtt
    28201 ggcggtggtg cggagaaagg gccgaatgaa aagtggataa tccccatcat taaagcgatg
    28261 gcaccgaata gcatgcccaa tgaactgatt ttattttgcg ctatgatacc catctgacct
    28321 ccttgtagcg gatggcaaaa gcatatcagg aaacgggcgg acgaaattct taaaaaaaac
    28381 cgctaaaggt agagcgttag tgaaattgca aaggagcaac aatgaaaatt tcccgcctcg
    28441 gagaagcgcc cgattaccgc ttctcgctgg ctaatgagcg tacttttctg gcgtggatcc
    28501 gtactgcact aggatttctg gcggcggggg ttgggcttga tcaacttgcg ccagatttcg
    28561 ccacgcccgt cattcgcgaa ctgctggcgt tgttgttgtg tctgttttcc ggcgggttag
    28621 cgatgtatgg ctacttgcgc tggttacgca atgaaaaagc gatgcgtctg aaagaggacc
    28681 tgccctacac caacagctta ttaatcatca gcttaatatt gatggtcgta gcagtgatcg
    28741 ttatgggact ggtgttgtat gccggatagc cgcaaagcca gacgcattgc cgacccaggg
    28801 ctgcagccgg agcgtacatc attggcgtgg tttcgtacca tgctgggcta cggcgcgttg
    28861 atggcgttgg ctatcaaaca caactggcac caggcgggca tgttattctg gatttcgatt
    28921 ggcatcctcg ccatcgtggc gctgatcctc tggcactaca ctcgtaatcg caatttaatg
    28981 gatgtcacga atagcgattt ttcccaattt cacgtagtcc gtgacaaatt tttgatctcc
    29041 ctcgcggtgt tatctctcgc aatactgttt gctgtaacgc atatacatca acttatcgta
    29101 tttattgaga gagtcgcatg acaggaagtc aggtcattga tgctgaagag gacaggcata
    29161 aactggttgt agagtataag gatactctgc aacctgccga tttttatcac aatttcaaac
    29221 agcggggcat ccgctccgta cagcttattc cccatcttga atttgatgac cggggcgata
    29281 tgacggccgc ttcggtgaca gcagagctgt gggggaaatt tttaattgcc ctgtttgagt
    29341 gttgggtgcg ggcagatatc agtcgtattt cgattgagct ttttgacgcc actttacaaa
    29401 aatggtgtgg cagcgaaaac ccgcacccgc gccgtgactg ccaggcgtgc gactggcatc
    29461 ggctgtgtcc gcacgctcgg gaggagacgc cagacaacgt gctttgtgcg ggctatcagg
    29521 cgttttattc ttactccgca ccgcatatgc gggtgatgcg tgatctgata aagcagcatc
    29581 gttctcccat ggagctgatg acgatgctgc ggtgatgtgc gcttaaacgg gctgcccctg
    29641 atgcgacatc cgcgtcgcca gcggtagcca gcacagcacg atcaacaatc ccattaaggt
    29701 catcaacaac cccaggctgc cctgaccggt ttgcggcaac atcgctgaga gcgacgccag
    29761 cacgccggaa ccaatgtttt gcagaccacc gaccagcgcg ccagccgtgc ccgccaggaa
    29821 ggggaacggc tccatcgcgc cgctggtcgc cagcggaaac agcatcccgg caccgaaaaa
    29881 gaatagcgcg gcgggaacaa gcagcgtcca gacattcatc acgccaaacc agtcggggat
    29941 ccacatcagc aagccagcca gcaggcagca gataaccgac tgccacatca acgttgagaa
    30001 gcgtttatta ggacgcccgg caaaccatgc cccaaaaaat gccgccggaa tcggcagaat
    30061 aaacaaaata ctgaccgtca tactgctcag ccctaacacc gcgcccatca gcacgccgga
    30121 gcaggcttca aaggcggcaa tcccggccag accgccaatc agcatcagca aataacagtt
    30181 aaaaccgccg ttaccgaaaa gcgttttata actggtaagc aggcgcgtgc gcggtgcatc
    30241 gaccggacgc gtttccggca tccagcgggc catactgaag gtcacgccag cacaaagaac
    30301 caacaaaaag agataacagg cgcgccagtt ccacatcgta tccagcagac cgccgattag
    30361 cggtgcgagc aacggactaa caagaatccc catgtttaac aggctgttag catggcgcaa
    30421 ctgtgtccgt tcatataaat cacgcggcaa agtacgcgcc attacgccgc caacgccggt
    30481 gcccatcccc tgcatcgcgc tggcggcaat caataccgtc aaactggagg tcgtgaccgc
    30541 gaccagcgtt gccagcataa aaatggacat tccgacgagg atcaccggtc ggcgacccac
    30601 acggtcggaa atcgggccat aaaacagctg tgagacaccg taagtcagca gataagcgcc
    30661 cattacgctc tgcaccgccc cttcacggac gttgagatcg cgcgccatat cggcaatagc
    30721 tggaatataa atggtttgcg ccatctgacc gacggccacg agtaatacca acatcaataa
    30781 caaattgacg tttctatgcc ttttcattat cacggatgct tttataaaaa taattagaat
    30841 aagtcactac gcattcccat aaatgcggga gaggaatcta tcacattgat gaatgttagc
    30901 cagatatgtg ccaggaatgg cgaaaagaaa aacggcagaa tatgtaaaca aaagcggcaa
    30961 taaatgttgc cggaagggat ggtaagtcgg ataagatgcg tcagcatcgc atccgacacc
    31021 aacccacaag cagaatataa aaggggagcg gtttcccgct cccctttggt gcgacttgaa
    31081 tctgaattac ttcaggtatt tcagaacagc atcaagtagt tgcagtgctg caacaatgag
    31141 tttgaggata agaatggcga tatccaccag gctcatacgc gtctcctgtg gttcaggagc
    31201 actgccagct gacgtacctt tccgctgcct gttgccagct gttgtgcacg cggtatctca
    31261 aggagaaacg ttgtgcttgt tgacataaca cagtgtgctc acgcggggtt aaggcgctga
    31321 cggcaccacc cgtttcagcc aggacttagt gcgccgggta acgatgcggc ccccgcataa
    31381 cacattgcgt acagtgatat tatagaagtt tactgtataa ataaacagta atagttggac
    31441 aaaacgcaaa ctgtgatcga tactcatcta tgcactaaat acgtcaaaat tcgtgccgaa
    31501 attgcgcgtt ctgcgcggaa caggtatact ttcagtgttg acataataca gtgtgctttg
    31561 cggttaccag ccgcaggcag ctgacgaaac ctcgctccgg cggggttttt tgttacctgc
    31621 aattcagtat aaaacgtgat caacccctca attttccctt gctgaaaaat tttccattgt
    31681 ctcccctgta aagctgtgct tgtataaata ttgttaaaca caaaaccaac aaggtcccca
    31741 atgactactt ccatgttcaa cgcaaagcta ctaccaactg cgccatccgc cgcagtggtc
    31801 gtcgtgcgtg tggtggtggt cgtcggcaat gcgccgtagg gactggaaca acacacgatt
    31861 ccaaaacccc gccggcgcaa accgggcggg gtttttcgtt taagcacctc ccggaaagtc
    31921 ggcccagaaa aaaaggactg gagcatggca agttcgggca caacatcgac gcgtaagcgc
    31981 tttaccggcg cagaatttat cgttcatttc ctggaacagc agggcattaa gattgtgacg
    32041 ggcattccgg gcggttctat cctgcctgtt tacgatgcct taagccaaag cacgcaaatc
    32101 cgccatattc tggctcgcca tgaacagggc gcggggttta tcgctcaggg aatggcgcgc
    32161 accgacggta aaccggcggt ctgtatggcc tgtagcgggc cgggtgcgac taacctggtg
    32221 accgccattg ccgatgcgcg gctggactcc atcccgctga tttgcatcac tggtcaggtt
    32281 cccgcctcga tgatcggcac cgacgccttc caggaagtcg atacctacgg catctctatc
    32341 cccatcacca aacacaacta tctggtcaga catatcgaag aactcccgca ggtcatgagc
    32401 gatgccttcc gcattgcgca atcaggccgc cctggcccgg tgtggataga cattcctaag
    32461 gatgtacaaa cggcagtttt tgagattgaa gctcagcccg cggtggcaga aaaagccgct
    32521 gcacccgcct ttagcgaaga aagcattcgt gacgcggctg cgatgattaa cgccgccaaa
    32581 cgcccggtgc tttatctggg cggcggtgtg atcaatgcgc ctgtgcgggt gcgtgaactg
    32641 gcggagaaag cgcaactgcc aactaccatg accttaatgg cgctgggtat gctgccaaaa
    32701 gcgcatccgc tgtcgctggg tatgctgggg atgcacggcg tgcgcagcac caactatatt
    32761 ttgcaggagg cggatttgtt gattgtgctc ggtgcgcgtt ttgatgaccg ggcgattggc
    32821 aaaaccgagc agttctgccc gaatgccaaa attattcatg tcgatatcga ccgcgcagag
    32881 ctgggtaaaa tcaagcaacc gcatgtggcg attcaggcgg atgttgatga cgtgctggcg
    32941 cagttgatcc cgctggtgga agcgcaaccg cgtgcagagt ggcaccagtt ggtggcggat
    33001 ttgcagcgcg aatttccgtg tccaatcccg aaagcgtgcg atccattaag ccattacggc
    33061 ctgatcaacg ccgttgccgc ctgtgtcgat gacaatgcga ttatcaccac cgacgttggt
    33121 cagcatcaga tgtggaccgc gcaagcctat ccgctcaatc gcccacgcca gtggctgacc
    33181 tctggcgggc tgggcacgat gggctttggc ctgcctgcgg cgattggcgc ggcgttggcg
    33241 aacccggatc gcaaagtgtt gtgtttctcc ggcgacggca gcctgatgat gaatattcag
    33301 gagatggcga ccgccagtga aaaccagctg gatgtcaaaa tcattctgat gaacaacgaa
    33361 gcgctggggc tggtgcatca gcaacagagt ctgttctaca agcagggcgt ttttgccgct
    33421 acctatccgg gaaaaatcaa ctttatgcag attgccgccg gattcggcct cgaaacctgt
    33481 gatttgaata acgaagccga tccgcaggct gcattgcaag aaatcatcaa tcgccctggt
    33541 ccggcgctga tccatgtgcg tattgatgcc gaagaaaaag tgtacccgat ggtgccgcca
    33601 ggtgcggcga atactgaaat ggtgggggaa taagccatgc aaaacacaac tcatgacaac
    33661 gtgattctgg aactcaccgt tcgcaaccat ccgggcgtaa tgacccacgt ttgtggcctt
    33721 tttgcccgtc gcgcttttaa cgttgagggc attctttgct tgccgattca ggacagcgac
    33781 aaaagccaca tttggctact ggtcaatgac gaccagcgtc tggagcagat gataagccag
    33841 atagacaagc tggaagatgt ggtgaaagtg cagcgtaatc agtccgatcc gacgatgttt
    33901 aacaagattg cggtgttttt tcagtaagga gcgacattaa accgctcggg ggggtactga
    33961 tgagcgattt ttgatagatc cgttcagata tatagggtga aattgtgcca gagaccatct
    34021 caggcacaat ttattagccc tgaatgttaa tttaagtcgc tgcaccagta atttgggggt
    34081 tcacagttag ggctaaaatt tccagcgatg gtgttaaatt ctgattgctg tctataccaa
    34141 tggtcatgac cagcattcta ttaattaatg gttcatcgtg aggtatatta tggcaagccc
    34201 tataaacagc ggaataatga tgtcaaatct ctgctcttcc acctttttgg ataaaaatat
    34261 aaatgtcgct gctgagcttg atattacaaa tagcgagaaa aaatattcgc ccggcagtaa
    34321 ttttgcaaaa tggatgttgc aggaaataaa gcgcttaata gcaaatatta tgtccggttc
    34381 tcgcagtgtt aacaccgaaa tccttaacta tttccaccct atgccaggca ctgaaaataa
    34441 tggcaatcgt acctggatgg cggcaactgg agagaacgaa tataccgtaa tcgaacaaac
    34501 aggtggcaaa tcctttaaag ttatacagtt tgatgcaggt gaaaaaaata caaaaaaaat
    34561 agtaaaagaa acatcatatt ctggagtggc tgtggcgact attatcaaat cgctgtctga
    34621 aaaaactgct gcgttagaaa cacattctgc tgacacggtt ttgaggaaaa aatta
//
LOCUS       DAZFKF010000044        34395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFKF010000044 DAZFKF010000000
VERSION     DAZFKF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.044"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(158..370)
                     /gene="rpmE"
                     /locus_tag="ACHKA1_002606"
     CDS             complement(158..370)
                     /gene="rpmE"
                     /locus_tag="ACHKA1_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO5680240.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            573..2771
                     /gene="priA"
                     /locus_tag="ACHKA1_002607"
     CDS             573..2771
                     /gene="priA"
                     /locus_tag="ACHKA1_002607"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO5680241.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2927..3952
                     /gene="cytR"
                     /locus_tag="ACHKA1_002608"
     CDS             2927..3952
                     /gene="cytR"
                     /locus_tag="ACHKA1_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO5680242.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4044..5003
                     /gene="ftsN"
                     /locus_tag="ACHKA1_002609"
     CDS             4044..5003
                     /gene="ftsN"
                     /locus_tag="ACHKA1_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO5680243.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5096..5626
                     /gene="hslV"
                     /locus_tag="ACHKA1_002610"
     CDS             5096..5626
                     /gene="hslV"
                     /locus_tag="ACHKA1_002610"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO5680244.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5636..6967
                     /gene="hslU"
                     /locus_tag="ACHKA1_002611"
     CDS             5636..6967
                     /gene="hslU"
                     /locus_tag="ACHKA1_002611"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO5680245.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7034..7960
                     /gene="menA"
                     /locus_tag="ACHKA1_002612"
     CDS             7034..7960
                     /gene="menA"
                     /locus_tag="ACHKA1_002612"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO5680246.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVVGIFLSQWAA"
     gene            8053..8538
                     /gene="rraA"
                     /locus_tag="ACHKA1_002613"
     CDS             8053..8538
                     /gene="rraA"
                     /locus_tag="ACHKA1_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO5680247.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            8598..9272
                     /locus_tag="ACHKA1_002614"
     CDS             8598..9272
                     /locus_tag="ACHKA1_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680248.1"
                     /translation="MQNIILLFIYLLLIAVNHFRYKFLLSGNAGEMILYFANVFFNPL
                     ALSFIIATIICITIKKRSSRSFIRGTCWLMGLFLIYSSYTVWERHSIWDYTFPEPGIT
                     VTVPSKQWVANIVKQGPTLVTRDAHAILAIVAFSQNELGVHSIEELTATQNGTAEMHV
                     CDVQGFACAYQEGVQTMSDGKVRHAIFMTLLDNTNLIQLVAAIDPDYLDEYQEEVMKM
                     MLSARQ"
     gene            9395..10021
                     /locus_tag="ACHKA1_002615"
     CDS             9395..10021
                     /locus_tag="ACHKA1_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680249.1"
                     /translation="MVGEYIGSVLLGPLLLPVLVSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(10060..10305)
                     /gene="zapB"
                     /locus_tag="ACHKA1_002616"
     CDS             complement(10060..10305)
                     /gene="zapB"
                     /locus_tag="ACHKA1_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO5680250.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            10731..11576
                     /gene="glpF"
                     /locus_tag="ACHKA1_002617"
     CDS             10731..11576
                     /gene="glpF"
                     /locus_tag="ACHKA1_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO5680251.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            11599..13107
                     /gene="glpK"
                     /locus_tag="ACHKA1_002618"
     CDS             11599..13107
                     /gene="glpK"
                     /locus_tag="ACHKA1_002618"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO5680252.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            13337..14347
                     /gene="glpX"
                     /locus_tag="ACHKA1_002619"
     CDS             13337..14347
                     /gene="glpX"
                     /locus_tag="ACHKA1_002619"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO5680253.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            14444..15190
                     /gene="fpr"
                     /locus_tag="ACHKA1_002620"
     CDS             14444..15190
                     /gene="fpr"
                     /locus_tag="ACHKA1_002620"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO5680254.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAVGLPMNKESSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(15195..15623)
                     /gene="uspD"
                     /locus_tag="ACHKA1_002621"
     CDS             complement(15195..15623)
                     /gene="uspD"
                     /locus_tag="ACHKA1_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO5680255.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(15650..15949)
                     /gene="yiiS"
                     /locus_tag="ACHKA1_002622"
     CDS             complement(15650..15949)
                     /gene="yiiS"
                     /locus_tag="ACHKA1_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO5680256.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(16161..16601)
                     /gene="yiiR"
                     /locus_tag="ACHKA1_002623"
     CDS             complement(16161..16601)
                     /gene="yiiR"
                     /locus_tag="ACHKA1_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5680257.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            16702..17301
                     /gene="yiiQ"
                     /locus_tag="ACHKA1_002624"
     CDS             16702..17301
                     /gene="yiiQ"
                     /locus_tag="ACHKA1_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO5680258.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            17409..18176
                     /gene="tpiA"
                     /locus_tag="ACHKA1_002625"
     CDS             17409..18176
                     /gene="tpiA"
                     /locus_tag="ACHKA1_002625"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO5680259.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(18231..18986)
                     /gene="cdh"
                     /locus_tag="ACHKA1_002626"
     CDS             complement(18231..18986)
                     /gene="cdh"
                     /locus_tag="ACHKA1_002626"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO5680260.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESVTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(19093..20082)
                     /gene="sbp"
                     /locus_tag="ACHKA1_002627"
     CDS             complement(19093..20082)
                     /gene="sbp"
                     /locus_tag="ACHKA1_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO5680261.1"
                     /translation="MNKWGVGLTFLLATTSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(20401..21363)
                     /gene="pfkA"
                     /locus_tag="ACHKA1_002628"
     CDS             complement(20401..21363)
                     /gene="pfkA"
                     /locus_tag="ACHKA1_002628"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO5680262.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(21544..22446)
                     /gene="fieF"
                     /locus_tag="ACHKA1_002629"
     CDS             complement(21544..22446)
                     /gene="fieF"
                     /locus_tag="ACHKA1_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO5680263.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(22595..23095)
                     /gene="cpxP"
                     /locus_tag="ACHKA1_002630"
     CDS             complement(22595..23095)
                     /gene="cpxP"
                     /locus_tag="ACHKA1_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO5680264.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            23245..23943
                     /gene="cpxR"
                     /locus_tag="ACHKA1_002631"
     CDS             23245..23943
                     /gene="cpxR"
                     /locus_tag="ACHKA1_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO5680265.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            23940..25313
                     /gene="cpxA"
                     /locus_tag="ACHKA1_002632"
     CDS             23940..25313
                     /gene="cpxA"
                     /locus_tag="ACHKA1_002632"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO5680266.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETLKANQLWGEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(25419..26093)
                     /gene="yiiM"
                     /locus_tag="ACHKA1_002633"
     CDS             complement(25419..26093)
                     /gene="yiiM"
                     /locus_tag="ACHKA1_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO5680267.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENISTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(26242..27225)
                     /gene="kdgT"
                     /locus_tag="ACHKA1_002634"
     CDS             complement(26242..27225)
                     /gene="kdgT"
                     /locus_tag="ACHKA1_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO5680268.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(27485..28105)
                     /gene="sodA"
                     /locus_tag="ACHKA1_002635"
     CDS             complement(27485..28105)
                     /gene="sodA"
                     /locus_tag="ACHKA1_002635"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO5680269.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            28390..29424
                     /gene="rhaT"
                     /locus_tag="ACHKA1_002636"
     CDS             28390..29424
                     /gene="rhaT"
                     /locus_tag="ACHKA1_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO5680270.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLISTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(29421..30269)
                     /gene="rhaR"
                     /locus_tag="ACHKA1_002637"
     CDS             complement(29421..30269)
                     /gene="rhaR"
                     /locus_tag="ACHKA1_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO5680271.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(30343..31179)
                     /gene="rhaS"
                     /locus_tag="ACHKA1_002638"
     CDS             complement(30343..31179)
                     /gene="rhaS"
                     /locus_tag="ACHKA1_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO5680272.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            31467..32936
                     /gene="rhaB"
                     /locus_tag="ACHKA1_002639"
     CDS             31467..32936
                     /gene="rhaB"
                     /locus_tag="ACHKA1_002639"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO5680273.1"
                     /translation="MTFRNCVAVDLGASSGRVMLTRYERECRSLTLREIHRFNNGLHS
                     QNGYITWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMTQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            32933..34192
                     /gene="rhaA"
                     /locus_tag="ACHKA1_002640"
     CDS             32933..34192
                     /gene="rhaA"
                     /locus_tag="ACHKA1_002640"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO5680274.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRAYEKEILSRRG"
BASE COUNT         8476 a         8803 c         8864 g         8252 t
ORIGIN      
        1 taaaatcctt ttgaaataaa taagaaccag aagcctagcg ccagtcaaca acaggaaatg
       61 ataatgagtt aacaccccgg aatttttcag aatttataaa aggcaaatta caggcacaaa
      121 aaaagcgccg tgcggcgctt ttttcggaaa tccggtctta tttgctgccc gggatgttga
      181 aacgcttgtt gaagcggtca acacggccac cggtagcaac atcacgctgt ttgccagtga
      241 agaacgggtg gcacttgctg cacacgtcga ggttcaggtc atgaccaacg gtggagcgga
      301 ttttcattac gttaccgcaa gagcagctag cagtaatttc ttcgtatttc gggtgaatat
      361 cttttttcat gggaaaacct cggtttaagg ccgcgtcgct cttccagccc taacgccaga
      421 caccacgcga tgttaaaagt atagcttcaa tacgatcatt tcgtacgaag gcgcgaaatc
      481 atacagaaat taaccagcgt atgcaaactg atccgcactc ttctacggca atgtgtatac
      541 taacccaccg aatttcaagt caggatgatg ctatgcccgt tgcccacgtt gccttgcccg
      601 ttccgcttcc tcgtaccttt gactatctgc tgccagaagg catgacggtt aaagctgggt
      661 gtcgcgtgcg cgtgccgttt ggcaaacagc aggagcgcat cgggattgtg gtatcagtta
      721 gcgatgccag cgaactgccg ctcaatgagc taaaagcggt agtcgaagtg ctggatagtg
      781 agccggtgtt tactcactcc gtctggcgat tgctgctatg ggcggcagat tattatcatc
      841 atccgattgg cgatgtgctg tttcatgcct tgccgatttt actacgccag gggcggcctg
      901 cggcgaacgc gccgatgtgg tactggtttg ccactgaaca aggccatgcg gtggatctga
      961 acagcctgaa acgttcgcca aagcaacaac aggcactggc ggcattgcgg caaggcaaaa
     1021 tctggcgcga ccaggttgcc acgcttgaat ttaatgatgc cgcgttacag gcgctgcgca
     1081 aaaaaggtct gtgtgattta gcaagtgaaa caccagagtt tagcgactgg cgaacgaact
     1141 atgccgtttc tggtgagcgg ttgcgattga ataccgaaca ggccaccgcc gttggcgcaa
     1201 ttcatagtgc ggcggatacc ttctctgcct ggctgctggc gggtgttaca ggttccggta
     1261 aaacagaggt ttatttgagc gtactggaaa acgtgctcgc tcagggcaaa caggcgctgg
     1321 tgatggtgcc ggaaatcggc ctgacaccgc aaactatcgc ccgttttcgt gaacgtttta
     1381 atgcccccgt agaagttctg cattccggcc tgaacgacag cgagcgtctt tcggcgtggt
     1441 tgaaagcgaa aaatggtgag gcggcgattg tgatcggcac ccgctccgcg ctgtttacgc
     1501 cgtttaaaaa tctcggtgtg attgtcattg atgaagagca cgacagttcc tacaagcagc
     1561 aggaaggctg gcgctatcat gcccgcgacc tggcggttta tcgtgcgcac agcgagcaaa
     1621 tcccgattat tcttggctcc gcaacacccg cactggaaac gttatgcaac gtccagcaga
     1681 aaaaataccg cctgctgcgc ctgacgcgtc gggcggggaa tgcgcgtccg gcaattcaac
     1741 acgtgctgga tttaaaaggt cagaaggtgc aggcggggct ggctccggcg ttaatcaccc
     1801 gtatgcgcca gcatttacag gccgacaacc aggttatcct ctttcttaac cgccgtggct
     1861 ttgcgcctgc cttgctgtgc cacgactgcg gctggattgc cgaatgtcca cgttgcgatc
     1921 actactacac gctgcatcag gcgcaacagc atttgcgctg tcaccactgt gacagccaac
     1981 gcccggtgcc gcgccagtgc ccttcctgcg gttccacgca cctggtaccc gtggggctgg
     2041 gcaccgaaca gcttgaacag acgctcgcgc cgttattccc tggtgtgccc atttctcgta
     2101 tcgaccgcga taccaccagc cgcaaaggag cgctggaaca acaactggcg gaagtgcatc
     2161 gcggcggcgc gaggatcttg attggcacgc aaatgctggc gaaaggtcac cactttccgg
     2221 atgtgacgct ggtagcgtta ctggatgtgg acggcgcgct gttttctgcc gattttcgct
     2281 cggcagagcg ttttgcccag ctatacaccc aggtagcagg ccgtgccggg cgtgcgggta
     2341 aacagggcga agtggtgctg caaacgcatc atccggaaca cccgctattg caaacgttgc
     2401 tctataaagg ctacgacgct tttgccgaac aggcactggc tgagcgacgc atgatgcagc
     2461 tgccgccgtg gaccagccat gtgattgtgc gtgcggaaga tcataacaat cagcatgcgc
     2521 cgctgttcct gcaacaactg cgtaatctga tcctctccag cccgctggca gacgacaaat
     2581 tgtgggttct cggtccggtt ccggcactgg cacctaaacg tggcggtcgc tggcgctggc
     2641 agatattgtt gcagcaccct tcccgcgtgc gcttgcaaca catcattaac ggtacgctgg
     2701 cactcatcaa tacaataccg gattcccgta aggtgaaatg ggtgctggat gttgatccga
     2761 ttgagggtta aaccgctcac gatgcgaggc ggatcgaaaa attcaatatt catcacactt
     2821 ttcatgaaaa ttctgtaacc gttttcacgc gctatctgct aaaaatgttg ccgatgtgaa
     2881 gtaaacatgg atgtagtacg cctgacgtgc caggcgagga gtgagtgtga aagcgaagaa
     2941 gcaggaaact gccgcgacca tgaaagacgt tgccctcaag gcaaaagtct ctacagcgac
     3001 cgtctcccga gcattaatga atcccgataa agtctctcag gccacccgta atcgggttga
     3061 aaaagcggcc cgggaagtgg gttatttacc gcagcctatg gggcgcaacg tcaagcgtaa
     3121 tgaatcccgc accattctgg tgattgtccc ggatatctgc gatcccttct ttagcgaaat
     3181 tattcgcggt atagaagtta cggcggcaaa tcacggatat ctggtgctga ttggcgactg
     3241 tgcgcatcaa aatcagcagg aaaaaacctt tatcgatttg atcatcacca agcaaattga
     3301 tggcatgttg ctgctgggtt caaggctgcc gtttgatgcc agcattgagg aacagcgtaa
     3361 tctgccgccg atggtgatgg cgaacgaatt tgcaccggaa ctggagctgc ctaccgttca
     3421 tatcgacaat ctgaccgccg catttgatgc agtaaattat ttatatgagc aagggcataa
     3481 acggattggc tgtatagccg gtcccgaaga gatgccgctg tgtcactacc gcctgcaagg
     3541 ttacgttcag gcgctgcgtc gctgcggcat tatggttgat ccgcaataca tcgcccgtgg
     3601 tgacttcacc ttcgaagccg gaagcaaagc gatgcagcag ctgcttgatc ttccacaacc
     3661 gcctactgct gtcttctgcc atagcgatgt gatggcgctc ggcgcacttt ctcaggcaaa
     3721 acgccagggg ctgaaagtcc cggaagacct ttccataatc ggttttgata acatcgacct
     3781 gacgcaattt tgtgatccgc cgctgacaac catcgcgcag ccgcgttacg aaatcggtcg
     3841 ggaagctatg ctgttattgc ttgatcaaat gcaggggcaa cacgttggca gtggctctcg
     3901 tttaatggac tgcgaactta tcatccgggg atcaacacgc gcgttacctt aaagtaaacc
     3961 tttaagactg ccgtgactgg tcaaaggccc gccgcttaag taacatggcg ggctgacgaa
     4021 cgaataaata cagcgaaacg atagtggcac aacgagatta tgtacgccgc agccaaccgg
     4081 caccttcgcg gcgaaaaaag agcacctcac ggaaaaagca acgaaatctg cctgcggttt
     4141 ctcccgctat ggtcgctatt gctgccgccg ttcttgtgac ctttatcggt ggtctgtact
     4201 tcattacgca tcacaagaaa gaagagtccg agacgctgca aagccagaaa gtgaccggaa
     4261 acggactacc accaaaacca gaagaacgct ggcgctacat caaagagctg gaaagtcgcc
     4321 agccgggtgt gcgtgcgccc acagaacctt ctgctggtgg tgaagtgaaa acgccggagc
     4381 aactgacacc agaacaacgt cagcttcttg aacaaatgca ggctgatatg cgccagcagc
     4441 caacgcagct ggttgaggtt ccgtggaatg aacagacgcc ggaacagcgt cagcaaacgc
     4501 tacagcgcca acgtcaggcg cagcaattag cggaacagca acgcctggct cagcagtcgc
     4561 gtacgactga acaaagctgg cagcagcaga cgcgtacgtc gcaagccgcg ccggtacagg
     4621 cgcaaccgcg ccagtctaaa ccggcttcta ctcagcagcc gtaccaggat ctgctgcaaa
     4681 cccctgcgca cacgaccgcg cagtcgaagc cgcagcaagc tgcgccggtt actcgtgccg
     4741 ctgacgcgcc aaaaccgacg gcggagaaaa aagacgaacg ccgctggatg gtgcagtgcg
     4801 gttcgttcag aggcgcggaa caggcagaga cggtacgtgc tcagctggcg ttcgaaggct
     4861 ttgactcaaa aattaccacc aacaatggct ggaatcgtgt ggtcattggc ccggtgaaag
     4921 gcaaagagaa cgcagacagc accctcaatc ggttgaagat ggcgggtcat acaaactgca
     4981 ttcggctcgc cgccgggggt tgaaaccctc aaaatccccc ccatctataa ttgcattatg
     5041 ccccgtactt ttgtacgggg tttgtactct gtattcgtaa ccaaggggtc agctcgtgac
     5101 aactatagta agcgtacgcc gtaacggcca tgtggtcatc gctggtgatg gtcaggccac
     5161 gttgggcaat accgtaatga aaggcaacgt gaaaaaggtc cgccgtctgt acaacgacaa
     5221 agtcatcgcg ggctttgcgg gcggtactgc ggatgctttt acgctgttcg aactgtttga
     5281 acgtaaactg gaaatgcatc agggccatct ggttaaagcc gccgttgagc tggcaaaaga
     5341 ctggcgtacc gatcgcatgt tgcgcaaact tgaagcactg ctggcagtcg cggatgaaac
     5401 cgcttcgctt atcatcaccg gtaacggtga cgtggtgcag ccagaaaacg atcttattgc
     5461 tatcggctcc ggcggccctt acgcccaggc tgcggcgcgc gcgctgttag aaaacactga
     5521 acttagcgcc cgtgaaattg ctgaaaaggc gttggatatt gcaggcgaca tttgcatcta
     5581 taccaaccat ttccacacca tcgaagaatt aagctacaaa gcgtaaggat ctcccatgtc
     5641 tgaaatgacc ccacgcgaaa tcgtcagcga actggataag cacatcatcg gccaggacaa
     5701 cgccaagcgt tctgtggcga ttgctctgcg taaccgctgg cgtcgcatgc agctcaacga
     5761 agagctgcgc catgaagtga ccccgaaaaa tatcctgatg atcggcccga ccggtgtcgg
     5821 taaaactgaa atcgcccgtc gtctggctaa gctggcgaat gcgccgttca tcaaagttga
     5881 agcgaccaaa ttcaccgaag tgggctacgt cggtaaggaa gtggattcta ttattcgcga
     5941 tctgaccgat gccgccgtga aaatggtacg cgtccaggct atcgagaaaa accgttatcg
     6001 cgctgaagaa ctggcagaag aacgtattct cgacgtgctg atcccacctg ctaaaaacaa
     6061 ctggggacag accgaacagc agcaggaacc gtccgctgct cgtcaggcat tccgcaaaaa
     6121 actgcgtgaa ggccagcttg atgacaaaga aatcgagatc gatcttgccg cagcaccgat
     6181 gggcgttgaa attatggctc ctccaggcat ggaagagatg accagccagc tgcagtccat
     6241 gttccagaac ctgggcggtc agaagcaaaa agcgcgtaag ctgaaaatca aagacgccat
     6301 gaagctgctg attgaagaag aagcggcgaa actggtgaac ccggaagagc tgaagcaaga
     6361 cgctatcgac gctgttgagc agcacgggat cgtgtttatc gacgaaatcg acaaaatctg
     6421 taagcgcggc gagtcttccg gtccggatgt ttctcgtgaa ggcgttcagc gagacctgct
     6481 gccgctggta gaaggttgca ccgtttccac caaacacggg atggtcaaaa ctgaccacat
     6541 tctgtttatc gcttctggcg cgttccagat tgcgaaaccg tctgacctga tcccggaact
     6601 gcaaggtcga ctgccaatcc gcgttgaact gcaggcactg accaccagcg acttcgagcg
     6661 tattctgacc gagccgaatg cctctatcac cgtgcagtac aaagcactga tggcgactga
     6721 aggcgtaaat atcgagttta ccgactccgg tattaaacgc atcgcggaag cggcatggca
     6781 ggtgaacgaa tctaccgaaa acatcggtgc tcgtcgttta cacactgttc tggagcgttt
     6841 aatggaagag atttcctacg acgccagcga tttaagcggt caaaatatca ctattgacgc
     6901 agattatgtg agcaaacatc tggatgcgtt ggtggcagat gaagatctga gccgttttat
     6961 cctataatcg cgttcaatca ttttcatcat tgtttgatgg ggctgaaagg ccccattttt
     7021 attggcgcgt attatgactg aacaacaaat tagccgaact caggcgtggc tggaaagttt
     7081 acgacctaaa accctccccc tcgcctttgc tgcaattatc gtcgggacgg cgctggcatg
     7141 gtggcaaggt cattttgatc cgctggtcgc cctgctggca ctgattaccg ccgggctatt
     7201 acagatcctt tctaacctcg ccaatgatta cggcgatgca gtaaaaggca gcgataaacc
     7261 tgaccgcatt gggccgctac gcggcatgca aaaaggggtc attacccagc aagagatgaa
     7321 acgggcgctc attattaccg tcgtgctcat ctgtctctct gggctggcac tggttgcagt
     7381 ggcatgccat acgctggctg attttgtcgg tttcctgatt cttggcgggt tgtcgatcat
     7441 tgccgctatc acctacaccg tgggcaatcg tccttatggt tatatcggtc tgggtgatat
     7501 ttccgtactg gttttctttg gctggttaag cgtcatgggg agctggtatt tacaggctca
     7561 tacattgatt ccggcactga tccttccggc gaccgcatgc ggcctgctgg caacggcagt
     7621 actgaacatt aataacctgc gtgatatcaa tagcgaccgc gaaaacggca aaaacacgct
     7681 ggtggtgcgc ttaggtgaag tgaacgcgcg tcgttatcat gcctgcctgc tgatgggctc
     7741 tctggtatgt ctggcgctgt ttaatctctt ttcgctgcat agcctgtggg gctggctgtt
     7801 cctgctggcg gcaccgttgc tggtgaagca agcccgttat gtgatgcggg aaatggaccc
     7861 ggtggcgatg cgaccgatgc tggaacgcac tgtcaaggga gcgttactga ctaacctgct
     7921 gtttgttgta gggatattcc taagccagtg ggcagcataa ctgacaaata tcaattaaca
     7981 attgatgatt ttgccaacag accacatagc gcgatatact gaaaattctc gcagcaactg
     8041 aatgttaagc ctatgaaata cgatacttcc gagctttgtg acatctatca agaagatgtt
     8101 aacgtcgtgg aaccgctgtt ctccaacttt ggcggacggg cgtcgtttgg cggacaaata
     8161 atcacggtaa aatgtttcga ggacaacggg ttgctgtacg atctgctcga acagaatggc
     8221 cgtggtcgtg ttcttgtcgt cgatggcggt ggttctgttc gtcgcgcact ggtcgatgct
     8281 gaactggcgc gtctggcagt acaaaatgaa tgggaaggtc tggtcattta cggcgcggtg
     8341 cgtcaggtag atgacctgga agagttggat atcggcatcc aggcgatggc ggcaattccg
     8401 gttggtgccg ctggcgaagg cattggcgaa agcgatgtcc gcgtcaattt tggcggtgtt
     8461 accttcttct ccggcgacca tctttatgcc gacaataccg ggattattct ttcagaagat
     8521 ccgctggata ttgaataagt atctgccatg cgttaaaagg ggaattaaat tccccttttt
     8581 tatacatgga cgtattcatg cagaatatta ttcttttatt tatatattta ttacttatag
     8641 ccgttaacca tttccgctac aaatttcttt tatctggcaa cgcgggtgaa atgattttat
     8701 attttgctaa cgtatttttt aacccacttg ccttatcatt tattatcgcc acaatcatct
     8761 gtataacgat aaaaaaaaga agcagccgca gctttattcg cggcacttgt tggttaatgg
     8821 gattatttct tatctatagc agctatacgg tgtgggaacg acacagcata tgggattata
     8881 ctttcccgga gccaggtatc accgtcacgg ttccgagcaa acagtgggtt gccaatatag
     8941 taaaacaagg accaaccctg gtgacccggg atgcccatgc tattttagct attgttgcct
     9001 tctcacaaaa tgaactcggt gtgcattcaa tcgaggaact taccgcgact caaaacggaa
     9061 ctgcagaaat gcatgtttgt gatgttcagg gatttgcctg cgcttatcag gaaggagtgc
     9121 aaacaatgag tgatggcaaa gtaagacatg ctattttcat gactctactg gataacacta
     9181 atttgataca actggtcgca gctatcgacc cagattattt ggacgaatat caagaagaag
     9241 taatgaaaat gatgctttct gctcgtcaat gatctgatgt gcatgttaat aataatggat
     9301 tgatatgaga tacgttatct tattaatact atggataata actgtttttc ttagtcatct
     9361 tcagcacagt aacttgctta ctggagatcc tgggatggtt ggcgagtata tcggttcagt
     9421 acttcttggg ccgttattac ttcccgtgct tgtgtcaggt attctgtgca cattcactaa
     9481 aaaaacgcgt aacttcgcca gttttactcg cgggtgctgt tgggtattgg gagtcctgct
     9541 tttatccaac gtcggcaata cgtttcgtct gttcaccccg tggcactata cgtttgagaa
     9601 ggcagctatt tccgtgactg tcccaaaccg acactggaat acggtttcta ttagtactga
     9661 taaaaccatt gatataagaa gtgaagataa tagcgtcttt atttctgctt tccgcctgcc
     9721 cgcaggacgt agcgccgatg actcgctgga agagttaaag aagatgcagc gagacaatct
     9781 caaggatcaa tacaatgaag aaaccttcca gtttcatgat tgtaatgcca aacatttcac
     9841 ctgtaagtat caggatgtgt tgataaattt tgatggtcag cagaaaagaa ccatcagtgt
     9901 ttatcttgaa gacaccccca gggctgtggg gatcattgca ttaatggagc cagatacggc
     9961 agataaatat cgccaacagg cgatggagat tatgttgtct gctaaaaata cagtgaagta
    10021 aaaaataagg cgccttgcgg cgcctctgcg tggaagagat taaacctctt ccatgcgacc
    10081 cagcagggcc tgcagacgtt cctgccagcc gttctgctgt tctttcagat ggttgttctc
    10141 acgctccagc tcttcgcgct gatgctgggc attttgaact tcctgcgaca gtgagttgtt
    10201 tttttctttc agctcttcga tttccatctg caacagagtg atggtatcaa tcgcctgctg
    10261 tacttttgct tccagtttct caaacacttc taatgacatt gtcatacctc tcctgaattg
    10321 caaggcgttg atggataaaa aatcctcgtc ccgattaccg gtgacgcctt aataaatacg
    10381 agcgcacttt agttagctcc gattgtatga agccgcgcca tcgctgtcca gcggcacgcc
    10441 ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat ccacaccaat
    10501 cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca tgtacaatca
    10561 gatggaataa atggcgcgat aacgctcatt ttatgacgaa gcacacacat tttaagttcg
    10621 atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag catcgtggag
    10681 gtccgtgact ttcacgcata caacaaacat taactcttca ggatccgatt atgagtcaaa
    10741 catcaacctt gaaaggccag tgcattgctg aattcctcgg taccgggttg ttgatttttt
    10801 tcggtgtggg ttgcgttgca gcactaaaag tcgctggtgc gtcttttggt cagtgggaaa
    10861 tcagtgtcat ttggggactg ggggtggcaa tggccatcta cctgaccgca ggggtttccg
    10921 gcgcgcatct taatcccgct gttaccattg cattgtggct gtttgcctgt ttcgacaagc
    10981 gcaaagttat tccttttatc gtttcacaag ttgccggcgc tttctgtgct gcggctttag
    11041 tttacgggct ttactacaat ttatttttcg acttcgagca gactcatcac attgttcgcg
    11101 gcagcgttga aagtgttgat ctggctggca ctttctctac ttaccctaat cctcatatca
    11161 attttgtgca ggctttcgca gttgagatgg tgattaccgc tattctgatg gggctgatcc
    11221 tggcgttaac ggacgatggc aacggtgtac cacgcggccc tttggctccc ttgctgattg
    11281 gtctactgat tgcggtcatt ggcgcatcta tgggcccatt gacgggtttt gccatgaacc
    11341 cagcgcgtga cttcggtccg aaagtctttg cctggctggc gggctggggc aatgtcgcct
    11401 ttaccggcgg cagagacatt ccttacttcc tggtgccgct ttttggccct atcgttggcg
    11461 cgattgtagg tgcatttgcc taccgcaaac tgattggtcg ccatttgcct tgcgatatct
    11521 gtgttgtgga agaaaaggag accacaactc cttcagaaca aaaagcttcg ctgtaatatg
    11581 actacgggac aattaaacat gactgaaaaa aaatatatcg ttgcgctcga ccagggcacc
    11641 accagctccc gcgcggtcgt aatggatcac gatgccaata tcattagcgt gtcgcagcgc
    11701 gaatttgagc aaatctaccc aaaaccaggc tgggtagaac acgacccaat ggaaatctgg
    11761 gccacccaaa gctccacgct ggtagaagtg ctggcgaaag ccgatatcag ttccgatcaa
    11821 attgcagcta tcggtattac gaaccagcgt gaaaccacta ttgtctggga aaaagaaacc
    11881 ggcaagccta tctataacgc cattgtctgg cagtgccgtc gtaccgcaga aatctgcgag
    11941 catttaaaac gtgacggttt agaagattat atccgcagca ataccggtct ggtgattgac
    12001 ccgtactttt ctggcaccaa agtgaagtgg atcctcgacc atgtggaagg ctctcgcgag
    12061 cgtgcgcgtc gtggtgaatt gctgtttggt acggttgata cgtggcttat ctggaaaatg
    12121 actcagggcc gtgtccatgt gaccgattat accaacgcct ctcgtaccat gttgttcaac
    12181 atccataccc tggactggga cgacaaaatg ctggaagtgc tggatattcc gcgcgagatg
    12241 ctgccagaag tgcgtcgttc ttccgaagtg tacggtcaga ctaacattgg cggcaaaggc
    12301 ggcacgcgta ttccaatctc cgggatcgcc ggtgaccagc aggccgcgct gtttggtcag
    12361 ttgtgcgtga aagaagggat ggcgaagaac acctatggca ctggctgctt tatgctgatg
    12421 aacactggcg agaaagcggt gaaatcagaa aacggcctgc tgaccaccat cgcctgcggc
    12481 ccgactggcg aagtgaacta tgcgttggaa ggtgcagtgt ttatggcagg cgcatccatt
    12541 cagtggctgc gcgatgagat gaagttgatt aacgacgcct acgattccga atatttcgcc
    12601 accaaagtgc aaaacaccaa tggtgtgtat gtggttccgg catttaccgg gctgggtgcg
    12661 ccatactggg acccgtatgc gcgcggggcg attttcggct taacgcgtgg agtgaacgct
    12721 aaccacatta tccgcgcgac gctggagtct attgcttatc agacgcgtga cgtactggaa
    12781 gcaatgcagg ccgactccgg tattcgtctg cacgccctgc gcgtggatgg cggcgcagta
    12841 gcaaacaatt tcctgatgca gttccagtcc gatattctcg gcacccgcgt tgagcgcccg
    12901 gaagttcgcg aagtgactgc attgggtgcg gcctatcttg ctggtctggc ggtcggtttc
    12961 tggcagaacc tcgacgagct gcaagagaaa gcggtgattg agcgcgagtt ccgtccaggc
    13021 atcgaaacca ctgagcgtaa ttaccgttac gcaggctgga aaaaagcggt caaacgcgcg
    13081 atggcgtggg aagaacacga cgagtaatgt caggcacctg aatcgattgc ctgatgctga
    13141 tcgcctgatg cgacgcttac gcgtcttatc aggcctacga gccgatgcca ttttgtgtag
    13201 gcgggataag gcgttcacgc cgcatccggc atcacgcaga ctctgaaacc gtgcgggggc
    13261 aaccccgcac acatcaataa tccctccctt cccctgtgct acacttcgcg ccattcctta
    13321 ctgcttagag tttgctatga gacgagaact tgccatcgaa ttttcccgcg tcaccgaatc
    13381 agcggcgctg gctggctaca aatggttagg acgcggcgat aaaaacaccg cggacggcgc
    13441 ggcggtaaac gccatgcgta ttatgctcaa ccaggtcaac attgacggca ccatcgtcat
    13501 tggtgaaggt gaaatcgacg aagcaccgat gctctacatt ggtgaaaaag tcggtactgg
    13561 tcgcggcgac gcggtagata ttgctgttga tccgattgaa ggcacgcgca tgacggcgat
    13621 gggtcaggct aacgcgctgg cggtgctggc agtaggcgat aaaggctgtt tcctcaacgc
    13681 gccagatatg tatatggaga agctgatcgt cggaccggga gccaaaggca ccattgatct
    13741 gaacctgccg ctggcggata acctgcgcaa tgttgcggcg gcgctcggca aaccgttgag
    13801 cgaactgacg gtaacgattc tggctaaacc acgccacgat gccgttatcg ctgaaatgca
    13861 gcaactcggc gtacgcgtat ttgctattcc ggatggcgac gttgcggcct caattctcac
    13921 ctgtatgcca gacagcgaag ttgacgtgct gtacggtatt ggtggcgcgc cggaaggcgt
    13981 ggtttctgcg gcggtgatcc gcgcattaga tggcgacatg aacggtcgtc tgctggcgcg
    14041 tcatgacgtc aaaggcgaca acgaagagaa tcgtcgcatt ggcgagcagg agctggcacg
    14101 ctgcaaagcg atgggcatcg aagccggtaa agtattgcgt ctgggcgata tggcgcgcag
    14161 cgataacgtc atcttctctg ccaccggtat taccaaaggc gatctgctgg aaggcattag
    14221 ccgcaaaggc aatatcgcga ctaccgaaac gctgctgatc cgcggcaagt cacgcaccat
    14281 tcgccgcatt cagtccatcc actatctgga tcgcaaagac ccggaaatgc aggtgcacat
    14341 cctctgattg atttgatcga ttgagccttc cagtccttcg ggactggaat ttttttgttc
    14401 ggagaacgaa gataaggcaa gtcaatcaaa acaggagaaa aacatggctg attgggtaac
    14461 aggcaaagtc actaaagtgc agaactggac cgacgccctg tttagtctca ccgttcacgc
    14521 tcccgtgctt ccgtttaccg ccgggcaatt taccaagctt ggccttgaaa tcgacggcga
    14581 acgcgtccag cgcgcctact cctatgttaa ctcgcccgat aatcccgatc tggagtttta
    14641 cctggtcacc gtccccgatg gcaaattaag cccacgattg gcggcactga aaccgggcga
    14701 tgaagtgcag gtggttagcg aagcagctgg cttctttgtt ctggatgaag taccagattg
    14761 cgaaacgcta tggatgctgg caaccggtac agcgattggc ccttatttat cgattctaca
    14821 actaggcaaa gatttagatc gcttcaaaaa tctggtcctg gtgcacgccg cacgttatgc
    14881 cgccgactta agctatttgc cactgatgca agaactggaa aaacgctacg aaggaaaact
    14941 gcgcattcag acggtggtca gtcgggaaac ggcagcgggt tcgctcaccg gacggatacc
    15001 ggcgttaatt gaaagtggtg aactggaaag cgcggttggc ctgccgatga ataaagaaag
    15061 cagccatgtg atgctgtgcg gcaatccaca gatggtgcgc gatacgcaac agttgctgaa
    15121 agagacccgg cagatgacga aacatttacg tcgccgaccg ggtcacatga cagcggagca
    15181 ttactggtaa gcggttactt atcgataaac ggcacgatca agagatccgc actcaactta
    15241 ttgatcatcc cgcgatatgc cggcatcaaa cggttgataa atgagtggtg atgaccacag
    15301 acaaggaggt cgcactgctc tttttgcata atttccagca gtgtttccgg catttctccg
    15361 cgttcaatac gcagttttgt cttcggccat tgaatatttt tcgtcagttt atacagcttg
    15421 ttatccgact tattcttcaa caattgaaga atatcttctg ttgcagggaa gtagataccc
    15481 gggtacaact cgcttaagcc atcatcaata tgaattaacg tcaggtgagc gtcattatgt
    15541 ctggcgagct ccagggcttt attcaccagt aaggcatctt cttcattccc ggaaattgcc
    15601 acgccaatgt gtttataagc catgtttaac tccttctaaa gcaactccat caagctaacg
    15661 aacgcagtga taactcaaaa ataatcatct cggcctggca tgagaaggtg aaggccgcat
    15721 caagctcaac ttgcccctcc agatccttaa tcttataggc cacgctggcc ccttcatctg
    15781 cagaggatgc agcggcggca agctctttca gtttggtcat aaaggactct gcttcttcgc
    15841 gggtggcaaa aaagcgcgaa aacttactgg tacagttgtc gttatcaatc accgtaccga
    15901 tatcaatcgc acatccttta gtactgcatt tatctacgac atctttcata gggcacctct
    15961 gtatattcgc ctctctgtcg attacgactt aagaataatg cgccgttaag ggaagaacaa
    16021 acaagtaatg atcttatgga ttaccatttt ttttgatatt gcttaaggtg aagagttcag
    16081 aaggattttc cgggcattgt catttgcatc gtggaaataa caatcccgga aaaaaatagc
    16141 agtaagagat atattaaagg ttaattactt gatttattgt cggctttata cttcacatcc
    16201 tgagtatctt taccatattt attttcgcct tgtgtgccaa caaacgcacc aagatcgata
    16261 agcatcatca ccagaatcaa cgtcgggaca aaacgcccta ccgcccattg ccagacaccc
    16321 ggtaaaatcg cccagttacc cgccagtagc atccacgcca caatcatcag aaatgcccat
    16381 gcgccggaac gcccgcgatc atgcaagcgc ttaacggtta ctgccgctgt tggccagagc
    16441 aagcacacaa ggcaaaacgc cgcggtctga atatcgagta aatttttacc cgccagtgag
    16501 aaaagcacca gcatgcctgc gaaccacagg cctatccaaa tccagaaatc acggcgtcca
    16561 atacgccctt taaatgagaa taaccattgc tgtatggtca tgtaagttcc ttgatggttg
    16621 tcttttccag gattctaccc ttttgacaag ggcgacagat atcgttttaa tcggagccag
    16681 tcataacaaa aggtactgtc aatgaagcca gggtgtacgc tgttttttct cttatgttct
    16741 gcattaaccg ttacaacaac ggcgcatgca caaacaccag atacggcaac gaccgcgcct
    16801 tatctgctgg ctggagcccc tactttcgat ctctccatca gccagtttcg agaagacttt
    16861 aacagccaga atcccagcct gccactgaac gaatttcgtg ccatcgacag cagtcccgac
    16921 aaagccaatc tcactcgtgc tgcaagtaaa attaacgaga acttgtatgc ttctacagcg
    16981 ctggagcgcg gtaccttaaa aatcaaaagc attcaaatga cctggctacc catccagggg
    17041 ccagagcaaa aagccgcgaa agcgaaagct caggaatata tggcagcggt gatccgcaca
    17101 ctcaccccat taatgaccaa aacacaaagc cagaaaaaac tgcagtcgct actaacggcg
    17161 gggaaaaaca aacgttatta caccgagaca gaaggtgcac tgcgttatgt tgtcgcggac
    17221 aacggcgaaa aggggctgac cttcgctgtt gaaccgatta agctggcgct atctgaatcg
    17281 cttgaaggtt tgaataaatg acaaaaagca aagcctttgt gccgatgaat ctctatactg
    17341 tttcacagac ctgctgccct gcggggcggc catcttcctt tattcgctta taagcgtgga
    17401 gaattaaaat gcgacatcct ttagtgatgg gtaactggaa actgaacggc agccgccaca
    17461 tggttcacga gctggtttct aacctgcgta aagagctggc aggtgttgct ggctgtgcgg
    17521 ttgcaatcgc accaccggaa atgtacatcg atatggcgaa gcgcgaagct gaaggcagcc
    17581 acatcatgct gggtgcgcaa aacgtggacc tgaacctgtc cggcgcattc accggtgaaa
    17641 cctctgctgc tatgctgaaa gacatcggcg cacagtacat catcatcggc cactctgaac
    17701 gtcgtactta ccacaaagag tctgacgaac tgatcgcgaa aaaattcgcg gtgctgaaag
    17761 agcagggtct gactccggtt ctgtgcatcg gtgaaaccga agctgaaaac gaagcgggca
    17821 aaactgaaga agtttgcgca cgtcagatcg acgcggtact gaaaactcag ggtgctgcgg
    17881 cattcgaagg tgcggttatc gcttacgaac ctgtatgggc aatcggtact ggcaaatctg
    17941 caactccggc tcaggcacag gctgttcaca aattcatccg tgaccacatc gctaaagttg
    18001 acgctaacat cgctgaacaa gtgatcattc agtacggcgg ctctgtaaac gcgtctaacg
    18061 ctgcagaact gtttgctcag ccggatatcg acggcgcgct ggttggtggt gcttctctga
    18121 aagctgacgc cttcgcagta atcgttaaag ctgcagaagc ggctaaacag gcttaagtct
    18181 gacaggtgcc ggatttcata tccggcactt actttcctta actcttcgcc ttaacgcaaa
    18241 atctcacact gatgatcctg aatttcctcg gctgaagcac ggttaagcgt cagtagattt
    18301 cgttgtgtcg ccagcaatac aaatgagtta tcactctgcc gtaccatcgc cagcccgtag
    18361 cttcccatat gttcccgcgc ctcaggtact tcttctgcca gcatcataaa tgggctgcgt
    18421 tgtaccagtt cgctttccgt tacccgacgc gccaggtatt catgcccgcg caaaccacct
    18481 ggcagtggca accagcggct gctgatgttc gccagattgt tatccagttg ttcgcgcaca
    18541 tcaggacgaa tacaagagat atgaatatga aaatggtttt gcgtacgccc ggtgcgggag
    18601 ttgatcgcca aagaaaccgc gcgatcggga accggctggc cgtatttttt gctcataaaa
    18661 tcacgcgcct gccaggccaa ccaaaagaag ttcggcgttg aaggatcggt caacaaagga
    18721 ctttcagtac cgttaatacg atacgttggc atcaacagat attgcagtgg gccattaaga
    18781 tcttttaaaa ccacgtatcc ggcattgggt ttgacttccg cacatggcga aggattttga
    18841 ttttgctgct gattgggcaa acattcctca aggacaatct tacgtaatgt aaccgactct
    18901 tcaccggtta atttccagta accaataccg gcagccacaa cggcgataac tatcatcacc
    18961 aaaaaaagaa gacccgcttt tttcatcttt ttttccctgt acctcaaaga gatgcaaggg
    19021 taacgcaaaa tcgtgacaaa taaaaaaccc ggtcgcgaga aaccaccggg tttgataatt
    19081 atcctggaga aatcagcgtt tgctgatctg atcgaacgta ccgccgttag caaaatgctc
    19141 tttttgcgct ttcgtccagc cgccgaactc ttcatcaatg gtgaataact tcagctttgg
    19201 aaacgcattt tcgtactttt tcgccacctc agcgtcgcgc ggacggtagt agtttttcgc
    19261 ggcaatttcc tgaccttctg gcgagtagag atatttcagg taggcttccg ccacctcttt
    19321 agtacctttt ttctcgacca ctttatcgac caccgacacg gttggctctg cgaggataga
    19381 ctcactcggc gtgacgattt cgaatttatc tttccccagt tcattcgctg ccagcagagc
    19441 ttcgttttcc caggcaatca gtacatcgcc aattccgcgc tcgacaaaag tgttagtgga
    19501 gccgcgcgcg ccagaatcca gaacttcgac gtttttatac agtgcccgaa caaaatcctg
    19561 tgcttttgcc tgatcgttgt tgttgtgatg cagcgcgtag ccccaggcag ccaggtaatt
    19621 ccagcgcgcg ccaccagagc ttttcggatt aggcgtgatc actgaaacac ccggtttaat
    19681 cagatcgttc cagtcatgga tctgcttcgg atttccctta cgtaccagga aaacaatggt
    19741 ggaagtgtac ggtgcggagt tatccggcag acgtttgatc cactctttat caatccgccc
    19801 gcgttccgca attgcgtcca cgtcataggc cagagccagc gtgacaacat cagcttcaat
    19861 accgttgatt accgacgtcg cttgtttacc tgaaccaccg tgtgactgac gaatcaccac
    19921 gttatcacca gtttgctgtt tccagtgggc gctgaatgcc ttgttgtact gttcgtacaa
    19981 ttcgcgcgtt ggatcatatg aaacgttaag aagctgaata tcctttgcca taacgctggt
    20041 tgtcgccagc aaaaatgtta accctacgcc ccacttgttc atcgcccgac tctcttatgt
    20101 tgtgttgtga tgagcaaagc gtgccagaag gttaaccaaa cattaaagaa taaaaaaaga
    20161 ttggctataa cttgcgggta tatgttgggg gttaaaaagg cggaagaaat ccgcctcata
    20221 ttgctgacaa agtgcgcttt atcgatgccg gatgcggcgc gaacgcctta tccggcctac
    20281 aaaagtttgt aaattcaata aattgtagaa ttcacgtagg cctgataagc gaagcgcatc
    20341 aggcattttt gcttctgtca tcggtttcag gctaaaggaa tctgcctttt tccgaaatca
    20401 ttaatacagt tttttcgcgc agtccagcca gtcgcctttg aacggacgct tcatgttttc
    20461 gatagcgtcg atgatgtcgt ggtgaaccag ctgttcgttc tggataccta cgcaacgacc
    20521 gccgtaacct gccagcagca gatcgatagc gtaagcgccc atacgggaag ccagaatacg
    20581 gtcgtaaggc accggagaac caccgcgctg gatgtggccc agcacagttg cgcgggtttc
    20641 acgaccggtt tctttctcga tgaaatgcgc cagttcgtca acatcacaca tatgttcggt
    20701 aatcgccacg atcgcgtgtt ttttaccttt cgcgataccc gctttgattt cgtttaccag
    20761 gtcttcacgg ctgaattcaa cttccggaac cacaacgaat tcacagccac cagcaatggc
    20821 cgcagccagc gtcagatcgc cacaataacg gcccatcact tccaccacgg aaatacgctg
    20881 gtgagaagaa gaggtgtcac gcagacggtc gatcgcttct acaacggtgc tcagcgcagt
    20941 gaagaaaccg atagtgtagt cagtgccttt gatgtcgttg tcgatagtgc caggcagacc
    21001 gatgcacggg aagcccattt cggtcagacg cattgcaccc atgtaggaac cgtcaccgcc
    21061 gataaccacc agcgcatcga tcccacgttt tttcaggttt tcgatagcca cggcgcggat
    21121 gttctcgtcg cggaattccg ggaaacgcgc agaaccgagg aacgtaccgc cacggttgat
    21181 catgtcagaa acgctgtaac ggtctagctg taccatacgg tcttcataca gacccagata
    21241 gccgtcataa atgcccatta cttccagacc ttctgtcagc gcagaacgaa caaccccgcg
    21301 aattgcggcg ttcatgcctg gcgcatcacc gccgcttgtc aacacaccga ttttcttaat
    21361 catgactacc tctgaacttt ggaatgcaaa atgaaatctg ttgccggaag tcttcttaca
    21421 catcgaagtg atccaacgaa tgtgcaaata gtataacaat cacttcctgc tgaattgatt
    21481 caggtcaggc caaatggcgg tattttatac acaaaatgcg ggtctggctc tcttttatac
    21541 tgattatgaa agcatagacc gtttaccctc cctgggtacg acggaacagg ggtcctgatg
    21601 gataatcaca tccgatcccg gaaaacgccg taaaatagcc tgctctacct gatccgccac
    21661 catatgtgcc tgaaccaaag gcagagagtc ttccatttcc aaatgaatct gaataaagcg
    21721 ggtcggccct gactgccgcg tgcgaagatc gtgagcgccg ctaacacccg gccaggaagt
    21781 cacgatatca ataatttctt gccgttcctc atcaggcaat gcgcgatcca gtaatgactg
    21841 taccgcctca tatcccatgc gtaacgcgct atataaaata tagatgccga ttcccaatgc
    21901 aaacagagca tcggcgcgat gccagccgta ccaggacaac cccagcgcga gcagaattgc
    21961 gccgttcatc ataacatcag actggtaatg tagcatatca gcccgcaccg cctggctttg
    22021 cgtccggcgc accacccaac gctgaaacga gacaaggata atcgtacaaa ttagcgccac
    22081 aattgtcacg ataaccccga cgcctggatc tgtcatcggt gttggagata tcagatgttg
    22141 aatacccgtc aaaaacagga atagtgccga accggagata aacatacttt gcgccagcgc
    22201 cgcgagggac tcagctttac cgtgaccaaa cgagtgatta tcgtcggcag gttgcaggga
    22261 atatcgcacc accagtaaat tcgtcaacga cgcgccgata tccaccagcg aatccaccag
    22321 cgcggcgaga atactcaccg acccggtata ccaccatgca aaaattttaa tcagcaatag
    22381 cagcgaagcc atcgccgtcg cagcaatcgc cgcccgactg accagccgtc cataagattg
    22441 attcataaat actcccgcta tcaactgacg ctagtataac ggaagcaaat catctgcaat
    22501 gcattaagca gcaggcaaat tgaggataaa aaaaaccccc acatcatgtg ggggaagaca
    22561 gggatggtgt ctatggcaag gaaaacaggg tttactactg ggaacgtgag ttgctactac
    22621 tcaatagctt caacgatgaa cttttttgcc attgcgtcac gtcgcgcaac tgctccattc
    22681 gttgttgatg tttctcgttt aaaaccgctt gctgctccgg cgttaacagg cgatacattt
    22741 ggttgcggac ttttgccatc tcaacctgac gagcaatttg ctcattcgcc attttttctg
    22801 cctgtgcgcg cacagcgttt tcatcaaaat tttctgcggt gacaaggcga tgcattgtct
    22861 ccagttcgct aacattaaca ggaggctgtt cgtgccgggc ctgttgcata agatctcgca
    22921 tctgctgacg ctgatgttcg gttaaactta tgccgtcgaa catatggctc tgcgtactgc
    22981 gctgcgtaag ttcttcaccc ggatgccagt tatcgcctga accgacttca gcagcgtggc
    23041 ttaatgaact gactgccagc gttgaggcca tgacggcagc ggtaactatg cgcatcattt
    23101 gctcccaaaa tctttctgtc gcgattcaac gatagagagt ttacgattca ggctgcaaac
    23161 atgcgtcagg gggtgtaaaa caacgtaaag tcatggatta gcgacgtctg atgacgtaat
    23221 ttctgcctcg gaggtattta aacaatgaat aaaatcctgt tagttgatga tgaccgagag
    23281 ctgacttccc tattaaagga gctgctcgag atggaaggct tcaacgtgat tgttgcccac
    23341 gatggggaac aggcgcttga tcttctggac gacagcattg atttactttt gcttgacgta
    23401 atgatgccga agaaaaatgg tatcgacaca ttaaaagcac ttcgccagac acaccagacg
    23461 cctgtcatta tgttgacggc gcgcggcagc gaacttgatc gcgttctcgg ccttgagctg
    23521 ggcgcagatg actatctccc gaaaccgttt aatgatcgtg agctggtggc acgtattcgc
    23581 gcgatcctgc gccgttcgca ctggagcgag caacagcaaa acaacgacaa cggttcaccg
    23641 acactggaag ttgatgcctt agtgctgaat ccaggccgtc aggaagccag cttcgacggg
    23701 caaacgctgg agttaaccgg tactgagttt accctgctct atttgctggc acagcatctg
    23761 ggtcaggtgg tttcccgtga acatttaagc caggaagtgc tgggcaaacg cctgacgcct
    23821 ttcgaccgcg ctatcgatat gcacatttcc aacctgcgtc gtaaactgcc ggatcgtaaa
    23881 gatggtcacc cgtggtttaa aaccttgcgt ggtcgcggct acctgatggt ttctgcttca
    23941 tgataggcag cttaaccgcg cgcatcttcg ccatcttctg gctgacgctg gcgctggtgt
    24001 tgatgttggt tttgatgtta cccaagctcg attcacgcca gatgaccgag cttctggata
    24061 gcgaacagcg tcagggtctg atgattgagc agcatgttga agcggagctg gcgaacgatc
    24121 cgcccaacga tttaatgtgg tggcggcgtc tgttccgggc gattgataag tgggcaccgc
    24181 caggacagcg tttgttatta gtgaccaccg aaggccgcgt gatcggcgct gaacgcagcg
    24241 aaatgcagat cattcgtaac tttattggtc aggccgataa cgccgatcat ccgcagaaga
    24301 aaaagtatgg ccgcgtggaa ctggtcggtc cgttctccgt gcgtgatggc gaagataatt
    24361 accaacttta tctgattcgt ccggccagca gttctcaatc cgatttcatt aacttactgt
    24421 ttgaccgccc gctattactg ctgattgtca ccatgttggt cagtacgccg ctgctgttgt
    24481 ggttggcctg gagtctggca aaaccggcgc gtaagctgaa aaacgctgcc gatgaagttg
    24541 cccagggaaa cttacgccag cacccggaac tggaagcggg gccacaggaa ttccttgccg
    24601 caggtgccag ttttaaccag atggtcaccg cgctggagcg tatgatgacc tcccagcagc
    24661 gtctgctttc tgatatctct cacgagctgc gcacgccact gacgcgtctg caactgggta
    24721 cggcgttact gcgccgtcgt agcggtgaaa gcaaggaact ggagcgtatt gaaaccgaag
    24781 cgcaacgtct ggacagcatg atcaacgatc tgttggtgat gtcacgtaat cagcaaaaaa
    24841 acgcgctggt aagcgagaca ttgaaagcta accagttgtg gggcgaagtg ctggataacg
    24901 cggcgttcga agccgagcaa atgggcaagt cgttgacagt taacttcccg cctgggccgt
    24961 ggccgctgta cggcaatccg aacgccctgg aaagtgcgct ggaaaacatt gttcgtaatg
    25021 ccctgcgtta ttcccatacg aagattgaag tgggctttgc ggtagataaa gacggtatca
    25081 ccattacggt ggacgacgat ggtcctggcg ttagcccgga agatcgcgaa cagattttcc
    25141 gtccgttcta tcggaccgat gaagcacgcg atcgtgaatc tggcggtaca ggtttggggc
    25201 tggcgattgt tgaaaccgcc attcagcagc atcgtggctg ggtgaaagca gaagacagcc
    25261 cgctgggcgg tttacggctg gtgatttggt tgccgctgta taagcggagt taaactccgc
    25321 atttgtaggc aggataaggc gtttacgccg catccggcat ttgagcagga tgcctgatgc
    25381 gacgctgata gcgtcttatc aggcctacac tccgggggtt attttcccca caaccggcgg
    25441 gaaaaatctt cgatcttgcc gctcagtcgg cgcttttgca tcgtccgcgt ccagcttttc
    25501 gataacccgg cggcggagag taaacggtga tactgatcat catcaaacgg catatgccat
    25561 gcgatggcgg cagcctcctg cacggtcaca tcgctgacac gggaaaccaa ctccagcggc
    25621 gcgtccgcag acactttccc tggcgctatc acgctataca accatcccac tttacccgtg
    25681 ttttgcatca actgcgctat atcgctgata tcaaaatggt aattgagttt atagcaaggc
    25741 gagcgcggct ggctgacctg aattaatgcc tctccccagc ggaaaatatc gccaatataa
    25801 acattacttt ccgtgagacc gtcggttgag atattttcgc caaacgcagg cgcaacaaac
    25861 aactccgcct gttccggaaa ttcccgcgcc cagtagagat agtgctcacg aggataatga
    25921 cacagcgctc tgtctggccc gccgtgaacc tttttctccg cctgctcgtc accttccagc
    25981 cccagctccg tcagcatcaa ctcaccatca acctggattt tggcaattgc gctgggtttg
    26041 ccttcgggat aagcctgaat cttgcctgtg tatacatcaa ccggatatcg catctgttcc
    26101 ctctccacga gaaatttccc cattagacca tatttatttt atgtcaggcg tgctgttttc
    26161 gcggtaagta tccaggcagg aactcaatag caaaccggac gaacgttagt tcatccggct
    26221 gtggggatta aggaagcagg tttatttcac cgtacctaaa atttcgattt tcgccgctct
    26281 ggctttgact ttacgtgacc agatagaggt aagaattggc accagaatcg aagtcacaat
    26341 gactgccgtc gctaccagcg aagttgctgc cggagccatc ggtttaaacg caggcaccat
    26401 ttctgcaatc agcacaggtg tcgctaccgc ggcccctgcg gaactggaag cggcaattcc
    26461 ggcagtgcca tcgccaccgc caatcaattt atcggcgata atcaacggaa taccggtcac
    26521 gataattact gccacaccca acaggatccc cagcaaacct gtctgggcaa ttacagtcaa
    26581 atcaatggta ttgcccagtg cgaaggcaaa gaatggaatc agcgtctgca ccgctttgct
    26641 gaaaaattct cgtaattcag ggtcaaggtt ccccagggca aagcccacca ggaacggtaa
    26701 tactgcgccg acgaaaacat gcggttcaaa cgaggcaatc cctgcagtgc ccagaataat
    26761 catcgtcatg agcggcccgg actccaacga catcaacaca aatgccccag cttcttcttt
    26821 tgtgccgtac tgctgcatga tggaagcgta aagtccgccg ttggtcatat ccatcgccgc
    26881 caccagcgcc agcgttgaaa gtccggcaaa gaatccaact tcaacaccat gttccggaat
    26941 aatgcgcgag gcaattgccg caaccaccca cgcgacggca attttagtta ccaccagcgt
    27001 accggattta cgcagtaccg ttcccgtcgc gcttaatttt attgacgccc ccatgcaaaa
    27061 aaaccacacc gccagaatgg gcaccgtacc ggtaatcatc ccgttggtga atgatccaaa
    27121 atatttcccc gcccccggcg agaaggtgtg gcacagtgcg ccaaggaata gcgggacgag
    27181 catcatcccc cccgggattt tctcaatcga gcgttttatc tgcatttcca tcacctgtcg
    27241 gcatattttt aagggctgat gcgtaattcc tcaatggagc atcagtgttg atggaaacga
    27301 agatagacat tcatttaatc aataaaagtg atcaaaacca catcaattga aacactgttt
    27361 tatttttatc ggatcattgt ttttattttg aaattgatca caaaaaaacc accgccgttg
    27421 gcgatggttc attttttaag ctgatatgcg gcctatacgc ctcattgcag caggcggcaa
    27481 atgattattt tttcgccgcg aaacgtgccg ctgcttcgtc ccagttcacc acgttccaga
    27541 actctttgat gtagtccgga cggcggttct ggaatttcag gtagtaagca tgttcccaca
    27601 catccaggcc cagaatcggg aagccggaag cgccagaaat agcttcaccc atcagcggag
    27661 aatcctggtt agcagtagaa accaccgcca gtttatcgcc tttcagcacc agccatgccc
    27721 agccggaacc aaagcgggaa gctgccgctt tttcaaattc tgctttgaag ttatctacgg
    27781 aaccgaaatc acgttcgata gccgctttca ggtcaccctg cagggtggtg ccttttttca
    27841 gacctttcca gaacaggctg tggttagcgt gaccgccagc gttgttgcgc agtacggttt
    27901 tcttgtctgc tggcagctgg tccagtttgg tgatcagctc ttcaaccggc aggttggcaa
    27961 attctggcag gctttccagc gccgcgttgg cgttgttgac gtaggtctga tggtgtttgg
    28021 tgtggtggat ttccatggtc tgcttatcga agtgcggttc cagggcatcg taagcatacg
    28081 gcagggatgg cagggtatag ctcatattca tctccagtat tgtcgggcgg ccgattgtta
    28141 atgccgcgta agcagttggt tcattatagt taattaaatg atattgaaaa tgattatcaa
    28201 tgccgtactt ttcgtaaggg tatggttttg caggaaaatg cccgagatgt gaagcaaatc
    28261 acccacttaa tgccgtgatt gccagtaaat cgacaacggc ggcaacaggc gaaaggttaa
    28321 tcgacagcac gatttttaca ctcatctcgt cggagatgtg acgcgacgaa aaatgatgag
    28381 gataagaaga tgagtaacgc gattacgatg gggatatttt ggcatttgat cggcgcggcc
    28441 agtgcagcct gtttttacgc tccgttcaaa aaagtaaaaa aatggtcatg ggaaaccatg
    28501 tggtcagtcg gtgggattgt ttcgtggatt attctgccgt gggccatcag cgccctgtta
    28561 ctaccgaatt tctgggcata ttacagctcg tttagtctct ctacgctgct gcctgttttt
    28621 ctgttcggcg ctatgtgggg gatcggtaat atcaactacg gcctgaccat gcgttatctc
    28681 ggcatgtcga tgggaattgg catcgccatt ggcattacgt tgattgtcgg tacgctgatg
    28741 acgccaatta tcaacggcaa tttcgatgtg ttgattagca ccgaaggcgg acgcatgacg
    28801 ttgctcggcg ttctggtggc gctgattggc gtagggattg taactcgagc cgggcagttg
    28861 aaagagcgca agatgggcat taaagccgaa gagttcaatc tgaaaaaagg gctggtgctg
    28921 gcggtgatgt gcggcatttt ctctgccggg atgtcctttg cgatgaacgc cgcaaaaccg
    28981 atgcatgaag ccgctgccgc acttggcgtc gatccactgt atgtcgctct gccaagctat
    29041 gttgtcatca tgggcggcgg cgcgatcatt aacctcggtt tctgttttat tcgtctggca
    29101 aaagtgaagg atttgtcgct aaaagccgac ttctcgctgg caaaaccgct gatcattcac
    29161 aatgtgttac tctcggcact gggcgggttg atgtggtatc tgcaattctt tttctatgcc
    29221 tggggccacg cccgcattcc ggcgcagtat gactacatca gttggatgct gcatatgagt
    29281 ttctatgtat tgtgcggcgg tatcgtcggg ctggtgctga aagagtggaa caatgcagga
    29341 cgccgtccgg taacggtgtt gagcctcggt tgtgtggtga ttattgtcgc cgctaacatc
    29401 gtcggcatcg gcatggcgaa ttaatctttc tgcgaattga gatgacgcca ctggctgggc
    29461 gtcatcccgg tttcccgggt aaacaccacc gaaaaatagt tactatcttc aaagccacat
    29521 tcggtcgaaa tatcactgat taacaggcgg ctatgctgga gaagatattg cgcatgacac
    29581 actctgacct gccgcagata ttgattgatg gtcattccag tctgctggcg aaattgctga
    29641 cgcaaaacgc gctcactgca cgatgcctca tcacaaaatt tatccagcgc aaagggactt
    29701 ttcaggctag ccgccaaccg ggtaatcagc ttatccagca acgtttcgct ggatgttggc
    29761 ggcaacgaat cactggtgta acgatggcga ttcagcaaca tcaccaactg cccgaacagc
    29821 aactctgcca tttcgttagc aaacggcaca tgctgactac tttcatgctc aagctgaccg
    29881 ataacctgcc gcgcctgtgc catccccatg ctacctaagc gccagtgtgg ttgccctgcg
    29941 ctggcgctaa atcccggaat cgccccctgc cagtcaagat tcagcttcag ccgctccggg
    30001 caataaataa tattctgcaa aaccagatcg ttaacggaag cgtaggagtg tttatcgtca
    30061 gcatgaatgt aaaagagatc gccacgggta atgcgataag ggcgatcgtt gagtacatgc
    30121 agaccattac cgcgccagac aatcaccagc tcacaaaaat catgtgtatg ttcagcaaag
    30181 acatcttgcg gataacggtc agccacagcg actgcctgct ggtcgctggc aaaaaaatca
    30241 tctttgagaa gttttaactg atgcgccacc gtggctacct cggccagaga acgaagttga
    30301 ttattcgcaa tatggcgtac aaatacgttg agaagattcg cgttattgca gaaagccatc
    30361 ccgtccctga cgaatatcac gcggtgacca gttaaactct cggcgaaaaa gcgtcgaaaa
    30421 gtggttactg tcgctgaatc cgcaatgata ggcgatgtca gtaacgctgg cctcgctgtg
    30481 acgtagcaga tgtcgagcct tcatcagtcg caggcggttc aggtaacgct gaggcgtcag
    30541 tcccgtttgc tgcttaagct gccgatgtag cgtacgcagc gaaagagaaa attgatccgc
    30601 cacggcatcc caattcacct catcggcaaa atggtcctcc agccaggcca gaagcaagtt
    30661 gagacgtgat gcgctgtttt ccaggttctc ctgcaaactg cttttacgca gcaagagcag
    30721 taattgcata aacaagatct cgcgactggc ggtcgagggt aaatcatttt ccccttcctg
    30781 ctgttccatc tgtgcaacca gctgtcgcac ctgctgcaat acgctgtggt taacgcgcca
    30841 gtgagacgga tactgcccat ccagctcttg tggcagcaac tgattcagcc cggcgagaaa
    30901 ctgaaatcga tccggcgagc gatacagcac attagtcaga cacagattat cggtatgttc
    30961 atacagatgc cgatcatggt cgcgcacaaa acagaccgtg ccaccggtga tggtataggg
    31021 ctgcccatta aacacatgaa tacccgtgcc atgttcgaca atcacaattt catgaaaatc
    31081 atgatgatgt tcaggaaaat ccgcctgcgg gagccggggt tctatcgcca cggacgcgtt
    31141 accagacgga aaaaaatcca cactatgtaa tacggtcata ctggcctcct gatgtcgtca
    31201 acacggcgaa atagtaatca cgaggtcagg ttcttacctt aaattttcga cggaaaacca
    31261 cgtaaaaaac gtcgattttt caagatacag cgtgaatttt caggaaatgc ggtgagcatc
    31321 acatcaccac aattcagcaa attgtgaaca tcatcacgtt catctttccc tggttgccaa
    31381 tggcccattt tcctgtcagt aacgagaagg tcgcgaattc aggcgctttt tagactggtc
    31441 gtaatgaaat tcagcaggat cacattatga cctttcgcaa ttgtgtcgcc gtcgatctcg
    31501 gcgcatccag tgggcgcgtg atgctgacgc gttacgagcg tgaatgccgc agcctgacgc
    31561 tgcgcgaaat ccatcgtttt aacaatgggc tgcatagtca gaacggctat atcacctggg
    31621 atgtggatag ccttgaaagt gccattcgcc ttggattaaa caaggtgtgc gaggaaggga
    31681 ttcgtatcga tagcattggg attgatacct ggggcgtgga ctttgtgctg ctcgaccaac
    31741 agggtcagcg tgtgggcctg cccgttgctt atcgcgacag ccgcaccaat ggcctgatga
    31801 cgcaggcaca acagcaactc ggcaaacgcg atatttatca gcgtagcggc atccagtttc
    31861 tgcccttcaa tacgctttat cagttgcgtg cgctgacgga gcaacaacct gaacttattc
    31921 cacacattgc tcacgctctg ctgatgccgg attacttcag ttatcgcctg accggcaaga
    31981 tgaactggga atataccaac gccacgacca cgcaactggt caatatcaat agcgacgact
    32041 gggacgagtc gctactggcg tggagcgggg ccaacaaagc ctggtttggt cgcccgacgc
    32101 atccgggtaa tgtcataggt cactggattt gcccgcaggg taatgagatt ccagtggtcg
    32161 ccgttgccag ccatgatacc gccagcgcgg ttatcgcctc gccgttaaac ggttcacgtg
    32221 ctgcttatct ctcttctggc acctggtcat tgatgggctt cgaaagccag acgccattta
    32281 ccaatgacac ggcactggca gccaacatca ccaatgaagg cggggcggaa ggtcgctatc
    32341 gggtgctgaa aaatattatg ggcttatggc tgcttcagcg agtgcttcag gagcggcaaa
    32401 tcaacgatct tccggcgctt atctccgcga cacaggcact tccggcttgc cgcttcatta
    32461 tcaatcccaa tgacgatcgc tttattaatc ctgagacgat gtgcagcgaa attcaggctg
    32521 cgtgtcggga aacggcgcaa ccaatcccgg aaagtgatgc tgaactggcg cgctgtattt
    32581 tcgacagtct ggcattgctg tatgccgatg tgttgcatga gctggcgcag ctacgcggtg
    32641 aagatttctc gcaactgcat attgtcggcg gcggctgcca gaacacgctg ctcaaccagc
    32701 tatgtgccga tgcctgcggt attcgggtga tcgccgggcc tgttgaagcc tcgacgctcg
    32761 gcaatatcgg catccagtta atgacgctgg atgaactcaa caatgtggat gatttccgtc
    32821 aggtcgtcag caccaccgcg aatctgacca cctttacccc taatcctgac agtgaaattg
    32881 cccactatgt ggcgcagctt cactctacac gacagacaaa ggagctttgc gcatgaccac
    32941 tcaactggaa caggcctggg agctggcgaa acagcgtttc gcggcggtgg ggattgatgt
    33001 cgaggaggcg ctgcgccaac ttgatcgttt acccgtttca atgcactgct ggcagggcga
    33061 tgatgtttcc ggttttgaaa acccggaagg ttcgctgacc ggtgggattc aggctactgg
    33121 taattatccg ggcaaagcgc gtaatgccag tgagctacga gccgatctgg aacaggctat
    33181 gcggctgatt ccggggccga aacggcttaa tttacatgcc atctatctgg aatcagacac
    33241 gccagtctcg cgcgatcaga tcaaaccaga gcacttcaaa aactgggttg aatgggcgaa
    33301 agccaatcag ctcggtctgg attttaaccc ctcctgtttt tcgcatccgc taagcgccga
    33361 tggctttacg ctttcccatg ccgacgacag cattcgccag ttctggattg atcactgcaa
    33421 agccagccgt cgcgtttcgg cctattttgg cgagcaactc ggcacaccat cggtgatgaa
    33481 catctggatc ccggatggta tgaaagatat caccgttgac cgtctcgccc cgcgtcagcg
    33541 tctgctggca gcactggatg aggtgatcag cgagaagcta aaccctgcgc accatatcga
    33601 cgccgttgag agcaaattgt ttggtattgg tgcggagagc tacacggttg gttccaatga
    33661 gttttacctg ggttatgcca ccagccgcca gacggcgctg tgcctggacg ccgggcattt
    33721 ccacccgact gaagtgattt ccgacaagat ttccgccgcc atgctgtatg tgccgcagtt
    33781 gctgctgcac gtcagccgtc cggttcgctg ggacagcgat cacgtggtgc tgctggatga
    33841 tgaaacccag gcgattgcca gtgagattgt tcgtcacgat ctgtttgacc gggtgcatat
    33901 cggcctcgac ttctttgatg cctctatcaa ccgcattgcc gcgtgggtca ttggtacacg
    33961 caatatgaaa aaagccctgc tgcgtgcgtt gctggaacct accgctgagc tgcgcaagct
    34021 ggaagcggcg ggcgattaca ctgcgcgtct ggcgctactg gaagaacaga aatcgttgcc
    34081 gtggcaggcg gtatgggaaa tgtattgtca gcgtcacgat acgccagcgg gtagcgaatg
    34141 gctggagaac gtgcgggctt atgagaaaga aattttgagt cgccgcgggt aaacactgcc
    34201 ggatgcggcg cgagcgcctt atccggccta caggtcggca acagttgtag gcctgataag
    34261 acgcgacagc gtcgcatcag gcattgattg ccggatgcgg cgcgagcgcc ttatccggcc
    34321 tacaggtcgg caacagttgt aggcctgata agacgcgaca gcgtcgcatc cggcattgat
    34381 tgccggatac ggcac
//
LOCUS       DAZFKF010000045        34339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFKF010000045 DAZFKF010000000
VERSION     DAZFKF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.045"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..92)
                     /locus_tag="ACHKA1_002641"
     CDS             complement(<1..92)
                     /locus_tag="ACHKA1_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680275.1"
                     /translation="MLGHISKFDGNNSLIKHGVVQGNNIVDFDLL"
     gene            complement(321..1241)
                     /gene="malM"
                     /locus_tag="ACHKA1_002642"
     CDS             complement(321..1241)
                     /gene="malM"
                     /locus_tag="ACHKA1_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO5680276.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVAGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1484..2824)
                     /gene="lamB"
                     /locus_tag="ACHKA1_002643"
     CDS             complement(1484..2824)
                     /gene="lamB"
                     /locus_tag="ACHKA1_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO5680277.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(2896..4011)
                     /gene="malK"
                     /locus_tag="ACHKA1_002644"
     CDS             complement(2896..4011)
                     /gene="malK"
                     /locus_tag="ACHKA1_002644"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO5680278.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4376..5566
                     /gene="malE"
                     /locus_tag="ACHKA1_002645"
     CDS             4376..5566
                     /gene="malE"
                     /locus_tag="ACHKA1_002645"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO5680279.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            5720..7264
                     /gene="malF"
                     /locus_tag="ACHKA1_002646"
     CDS             5720..7264
                     /gene="malF"
                     /locus_tag="ACHKA1_002646"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO5680280.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            7279..8169
                     /gene="malG"
                     /locus_tag="ACHKA1_002647"
     CDS             7279..8169
                     /gene="malG"
                     /locus_tag="ACHKA1_002647"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO5680281.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8541..10016
                     /gene="xylE"
                     /locus_tag="ACHKA1_002648"
     CDS             8541..10016
                     /gene="xylE"
                     /locus_tag="ACHKA1_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO5680282.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSSRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10060..10470)
                     /gene="psiE"
                     /locus_tag="ACHKA1_002649"
     CDS             complement(10060..10470)
                     /gene="psiE"
                     /locus_tag="ACHKA1_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO5680283.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10596..10739)
                     /locus_tag="ACHKA1_002650"
     CDS             complement(10596..10739)
                     /locus_tag="ACHKA1_002650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680284.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            10740..11018
                     /locus_tag="ACHKA1_002651"
     CDS             10740..11018
                     /locus_tag="ACHKA1_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680285.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11065..13161)
                     /gene="yjbH"
                     /locus_tag="ACHKA1_002652"
     CDS             complement(11065..13161)
                     /gene="yjbH"
                     /locus_tag="ACHKA1_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO5680286.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGVDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13161..13898)
                     /gene="yjbG"
                     /locus_tag="ACHKA1_002653"
     CDS             complement(13161..13898)
                     /gene="yjbG"
                     /locus_tag="ACHKA1_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO5680287.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSEMPDALNADILQTLTQRIPQ"
     gene            complement(13895..14533)
                     /gene="yjbF"
                     /locus_tag="ACHKA1_002654"
     CDS             complement(13895..14533)
                     /gene="yjbF"
                     /locus_tag="ACHKA1_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO5680288.1"
                     /translation="MKRPAFILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(14647..14889)
                     /gene="yjbE"
                     /locus_tag="ACHKA1_002655"
     CDS             complement(14647..14889)
                     /gene="yjbE"
                     /locus_tag="ACHKA1_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO5680289.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15401..17050)
                     /gene="pgi"
                     /locus_tag="ACHKA1_002656"
     CDS             complement(15401..17050)
                     /gene="pgi"
                     /locus_tag="ACHKA1_002656"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO5680290.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSQAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      17287..17483
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17575..18924
                     /gene="lysC"
                     /locus_tag="ACHKA1_002657"
     CDS             17575..18924
                     /gene="lysC"
                     /locus_tag="ACHKA1_002657"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO5680291.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLSMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            19177..19449
                     /gene="yjbD"
                     /locus_tag="ACHKA1_002658"
     CDS             19177..19449
                     /gene="yjbD"
                     /locus_tag="ACHKA1_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO5680292.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19450..20322)
                     /gene="rluF"
                     /locus_tag="ACHKA1_002659"
     CDS             complement(19450..20322)
                     /gene="rluF"
                     /locus_tag="ACHKA1_002659"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO5680293.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKTKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            20670..21617
                     /locus_tag="ACHKA1_002660"
     CDS             20670..21617
                     /locus_tag="ACHKA1_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO5680294.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            21687..22493
                     /locus_tag="ACHKA1_002661"
     CDS             21687..22493
                     /locus_tag="ACHKA1_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5680295.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22503..22910
                     /locus_tag="ACHKA1_002662"
     CDS             22503..22910
                     /locus_tag="ACHKA1_002662"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO5680296.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNTNVEAVEFVPGENAGDIV
                     AKLKKLVSIHSQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            22910..23404
                     /locus_tag="ACHKA1_002663"
     CDS             22910..23404
                     /locus_tag="ACHKA1_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO5680297.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            23470..24267
                     /locus_tag="ACHKA1_002664"
     CDS             23470..24267
                     /locus_tag="ACHKA1_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO5680298.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24278..25102
                     /locus_tag="ACHKA1_002665"
     CDS             24278..25102
                     /locus_tag="ACHKA1_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO5680299.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25154..26383
                     /gene="sorE"
                     /locus_tag="ACHKA1_002666"
     CDS             25154..26383
                     /gene="sorE"
                     /locus_tag="ACHKA1_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO5680300.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSMVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSESQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            26765..27454
                     /gene="pepE"
                     /locus_tag="ACHKA1_002667"
     CDS             26765..27454
                     /gene="pepE"
                     /locus_tag="ACHKA1_002667"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO5680301.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(27545..29176)
                     /gene="yjbB"
                     /locus_tag="ACHKA1_002668"
     CDS             complement(27545..29176)
                     /gene="yjbB"
                     /locus_tag="ACHKA1_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO5680302.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLAMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29396..33079)
                     /gene="metH"
                     /locus_tag="ACHKA1_002669"
     CDS             complement(29396..33079)
                     /gene="metH"
                     /locus_tag="ACHKA1_002669"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO5680303.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRVRKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLETARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33279..34103
                     /gene="iclR"
                     /locus_tag="ACHKA1_002670"
     CDS             33279..34103
                     /gene="iclR"
                     /locus_tag="ACHKA1_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO5680304.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         8097 a         8973 c         8903 g         8366 t
ORIGIN      
        1 agtaaatcaa aatctactat attatttcct tgcaccacac catgttttat caaagagtta
       61 ttgccgtcaa actttgagat atgacctaac ataaaacctc tgcattacag aaatgaaata
      121 acctggaaaa gtagcctgaa tgtccagaca ctaccggcga aaggaaaaga atctctgata
      181 aggcattgag ataatggata ttcttagtga agaagaagtg agttactata aaaaatcgct
      241 catatcccag gaatgaagca taaaaaaagg tgcgccagga gacgcaccag ttgttgcaaa
      301 atcagcactg tggggcgtaa ttaccccttg ccttttacac tgctgataaa ggtggaacgg
      361 gcagatgtcg atcccaggcg ttcagcttca tcaagcagtt ttaacgcctt atcaacatca
      421 cctttcgcga cagcgttttt gatcgcggta ttaaaataac tttccgtgtc gttgagcatc
      481 ggctcgcttt tcttcaccgg caccggagcg ggtgcagcca cagctggtgc cgccgtgttg
      541 cctaccgtaa ccggagctgg agcggaagaa ccaaataaag gcccgaccaa cacgctggag
      601 ctggagttcg ttttcacttt cagtttcagt aagccatcgg tggtatgacg agcaaccgga
      661 tcgggaatat ccgggatcga gttaccaacg cctttggcat aggctttagc cgggtcgagc
      721 agttgggtcg tctgctggag atctttttcc gtggtaaaga ccagaacata aagtttttgc
      781 tgccccaacg ccggtgtcag gcgcataacg ccttccagcc gatctgcact catcacgcct
      841 ggttcctggt aggtgaaata actgctggga aagaaggctg atggggtcat gttctgatca
      901 agaatcagca cgttcggcgc aaatacgctg gtttgtttgt tcacttcgct ggtcagcgtc
      961 agggtcagtt cgccaatgtt tgccgggacg ctgtacgcag caaccggacc actgatgcca
     1021 gcaacgttca gttgttggcc gccagtcgcc agttgggtgg tctgggtttt agattgatcg
     1081 accggtgtcc aggtgagttg ttgcagcgcg gcagatggaa tggctggcgc gtcgctggtg
     1141 ttttgcggta cgtagttaac atcggcaagg ctgattccag gcgcgcttgc cagcaaccct
     1201 gctgataaac agagggcgat gagactttta ttcattttca ttgttttcac ctcaaaatct
     1261 ggagctcagc ggtagccagg caatagcgcg ctaaacccga taatcagagg ggcttgcgcc
     1321 cctttcagat aatgacaacc tgtttttatg ccggatgcgg cgtaaacgcc ttatccggcc
     1381 tacatttgac agccgttgta ggcctgataa gacgcgcaag cgtcgcatca ggcgttggat
     1441 gccggatgcg gcgcaagcac cttatccggc ccaggttttg ctattaccac cagatttcca
     1501 tctgggcacc gaaggtccac tcgtcgctgt cgccacgacc gaagctgccg ccgttgaaat
     1561 cagcaggaac ggctttgccg tagttcgggt taaccttgct atcgccgttg tagtcgtaac
     1621 cccatttctc atcccacttg gcgtaggttg cgaagacacg aatagccggg cgtgaccaga
     1681 tgctgtcgcc agcctgccat tgttgtgcga gggtaatttt gtactgattg ttcttgtcgc
     1741 cggtgcgctg ggattcgacg ttgtcgtagc cgatttccat cacggtgctc atgattggcg
     1801 tccacttgta catcgggcga ataccgacgg tccaccactt ggtgccgttg tcgttatccc
     1861 agttgatatc ctggtacata cccacgtaca tcatgtccca gttgtcgccc atggagatcg
     1921 caccgtggtc gaggatacgc agcatgtgac cgttgttgtt gatattgtag gcaaattttt
     1981 cgttatcaaa tgcaacgcca gaaccctgcg acagaccttt accctgcgag gtcatcgagt
     2041 cagtagcgta ctgaacaaca aacttgttaa agcccttcag gacactctga gtatgttcag
     2101 cagtgaataa ccagccgtct ttcgatgcgc catcaaccag acgatagtta tcacgcaggt
     2161 tggcacgacc gtagtcgaca cccagttcta atgtgccgcc cgggttgatt tccatctgcg
     2221 ctaaacgcac atcgaaaacg tcgttcgcgg tttcgttggt atagtcataa atattgttgc
     2281 tggcgaaaga ggaagaacca cctgcttcag aggagcgggt tgctgccaga gagagtttac
     2341 cgaagccaac atcgatgttt tccagaccgg caccaggacc agaaatatcc cagtagtaga
     2401 agtcgatcat atgaacgtca tgacgttggt agaagcgctt acctgcccag atggtggagc
     2461 ctggcagcca ttcgatcagg tttttaccct gcacgtttgc ttcacggaag gccgggtcgg
     2521 tagcttccca gtcattctgt tgcgcgacgg aataggccac gttagtgtcg aaatagaagc
     2581 tcttatcgcc ctctttccac acttcctgac ccaattttaa ttcagcataa gtttcacatt
     2641 cgttgccaag acggtattta ctttgagcac cggtagtctg gaaacactgt tgttcaccgc
     2701 cgctacctgt ccagccaata ccggaacgtg catagccgtg gaaatcaaca gccattgcct
     2761 gagcagacat tacgcccgct gcgacggcaa ccgccagagg aagtttgcgc agagtaatca
     2821 tcattctatc tcctgagtca ttgcttttct tttttcacat cacctgtgac aggctttgtg
     2881 tgttttgtgg ggtgcttaaa cgcccggctc cttatgcagt cgacgacatg cagtgccatc
     2941 ctcacggaac agatggcaac gctctggcgg caggccgata gcgaatgtgg caccttcttc
     3001 taccaacacc acgtcgttct ggcggtacac caggttttga cgaatggaag ggatctggat
     3061 atggatttgg gtttcgttgc cgagttgctc gacgacctga acttcaccct caaggatgac
     3121 gtcagcgata tcactcggca gtagatgttc cgggcgaata cccagcgaca tattggctcc
     3181 aacctggaca tcacggcttt caactggcag ccagacttgc tgacgatttg gcatcggcag
     3241 ctccacctgc acttgatcga ttgcggtggc ggtcactttt accggcagga agttcatctt
     3301 tggcgaaccg ataaatccgg cgacaaaacg gtctgccgga tagtggtaca gctccagcgg
     3361 tttcccaacc tgcgccacgc gaccggcgtc cagcaccacg attttgtcgg ccagcgtcat
     3421 cgcttcgacc tgatcgtggg tgacgtaaat cattgtgcgg cccaggcgtt tatgcagacg
     3481 ggagatttcg atacgcattt gcacacgcag tgcagcatcg aggttggaga gcggttcatc
     3541 gagcaaaaat acgcttggct cggccaccag cgtacggcca atcgccacgc gctgacgctg
     3601 accaccggag agcgctttcg gtttgcgatc cagcaaatgc gccagttgca gcacttccgc
     3661 cacctggttg acgcgttggt taatcacctc tttttttgcg ccagccagtt tcaggccaaa
     3721 tgacatgttt tctgctactg acaggtgggg atagagcgcg taagactgaa acaccatacc
     3781 aacgccgcgt tctgctggcg gagtgtcatt catccgtttc tcaccgatga acaagtcgcc
     3841 gctggtgatc gtctcaagcc cggcaatcat gcgcagtaaa gtcgatttac cgcagccaga
     3901 cggtccgaca aacaccacaa attcaccttc atggatatcg agattgatat ctttcgatac
     3961 cacgacctca ccccaggctt tcgttacatt ttgcagctgt acgctcgcca tgcccttctc
     4021 cctttgtaac aacctgtcat cgacagcaac attcatgatg ggctgactat gcgtcatcag
     4081 gagatggctt aaatcctcca ccccctggct tttttatggg ggaggaggcg ggaggatgag
     4141 aacgcggctt ctgtgaacta aaccgaggtc atgtaaggaa tttcgtgatg ttgcttgcaa
     4201 aaatcgtggc gattttatgt gcgcatctcc acattaccgc caattctgta acagagatca
     4261 cacaaagcaa cggtggggcg taggggcaag gaggatggaa agaggttgcc gtataaagaa
     4321 actagagtcc gtttaggtgt tttcacgagc acttcaccaa caaggaccat agattatgaa
     4381 aataaaaaca ggtgcacgca tcctcgcatt atccgcatta acgacgatga tgttttccgc
     4441 ctcggctctc gccaaaatcg aagaaggtaa actggtaatc tggattaacg gcgataaagg
     4501 ctataacggt ctcgctgaag tcggtaagaa attcgagaaa gataccggaa ttaaagtcac
     4561 cgttgagcat ccggataaac tggaagagaa attccctcag gttgcggcaa ctggcgatgg
     4621 ccctgacatt atcttctggg cacacgaccg ctttggtggc tacgctcaat ctggcctgtt
     4681 ggctgaaatc accccggaca aagcgttcca ggacaagctg tatccgttta cctgggatgc
     4741 cgtacgttac aacggcaagc tgattgctta cccgatcgct gttgaagcgt tatcgctgat
     4801 ttataacaaa gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga
     4861 taaagaactg aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag aaccgtactt
     4921 cacctggccg ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa acggcaagta
     4981 cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga ccttcctggt
     5041 tgacctgatt aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc
     5101 ctttaataaa ggcgaaacag cgatgactat caacggcccg tgggcatggt ccaacatcga
     5161 caccagtaaa gtgaattatg gtgtaacggt actgccgacc ttcaagggtc aaccatccaa
     5221 gccgttcgtt ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca aagagctggc
     5281 gaaagagttc ctcgaaaact atctgctgac tgacgaaggt ctggaagcgg ttaataaaga
     5341 caaaccgctg ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga aagatccacg
     5401 tattgcagcc accatggaaa acgcccagaa aggtgaaatc atgccgaaca tcccgcagat
     5461 gtccgctttc tggtatgcag tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac
     5521 tgtcgatgaa gccctgaaag acgcgcagac tcgtatcacc aagtaatgct gtgaaatgcc
     5581 ggatgcggcg tgaacgcctt gtccggccta caaaaccgaa acgtatgtag gcctgataag
     5641 acgcgtcagc gtcgcatcag gcagttgttg tcggataagg cgtgaaagcc ttatccgtcc
     5701 tggaatgagg aagaacccca tggatgtcat taaaaagaaa cattggtggc aaagcgacgc
     5761 gctgaaatgg tcagtgttag gtctgctcgg cctgctggtg ggttaccttg ttgttttaat
     5821 gtacgcacaa ggggaatacc tgttcgccat taccacgctg atattgagtt cagcggggct
     5881 gtatattttc gccaatcgta aagcctacgc ctggcgctat gtttacccgg gaatggctgg
     5941 aatgggatta ttcgtcctct tccctctggt ctgcaccatc gccattgcct tcacaaacta
     6001 cagcagcact aaccagctga cttttgaacg tgcacaggaa gtgttgttag atcgctcctg
     6061 gcaagcaggc aaaacctata actttggtct ttacccggcg ggcgatgagt ggcaactggc
     6121 gctcagcgac ggcgaaaccg gcaaaaatta cctctccgac gcttttaaat ttggcggaga
     6181 gcaaaaactg caactgaaag aaacgaccgc ccagcccgaa ggcgaacgcg cgaatctacg
     6241 cgtgattacc cagaatcgtc aggcgctgag tgacattacc gccattctgc cggatggcaa
     6301 caaagtgatg atgagctccc tgcgccagtt ttctggcacg cagccgctct acacactcga
     6361 cggtgacggc acgttgacga ataatcagag cggcgtgaaa tatcgtccga ataaccaaat
     6421 tggcttttac cagtccatta ccgccgacgg caactggggc gatgaaaagc taagccccgg
     6481 ttacaccgtg actactggct ggaaaaactt tacccgcgtc ttcaccgacg aaggcattca
     6541 gaaaccgttc ctcgccattt tcgtctggac cgtggtgttc tcgctgatca ctgtcttttt
     6601 aacagtggca gtcggcatgg ttctggcgtg tctggtgcag tgggaagcgt tgcgcggcaa
     6661 agcggtctat cgcgtcctgc tgattctgcc ctacgcggtg ccatcgttca tttcaatctt
     6721 gattttcaaa gggttgttta accagagctt cggtgagatc aacatgatgt tgagcgcgct
     6781 gtttggcgtg aagcccgcct ggttcagcga tccgaccacc gcccgaacga tgctgattat
     6841 cgtcaatacc tggctgggtt atccgtacat gatgatcctc tgcatgggct tgctgaaagc
     6901 gattccggac gatttgtatg aagcctcagc aatggatggt gcaggtccgt tccagaactt
     6961 ctttaagatt acgctgccgc tgctgattaa accgctgacg ccgctgatga tcgccagctt
     7021 cgcctttaac tttaacaact tcgtgctgat tcaactgtta accaacggcg gcccggatcg
     7081 tcttggcacg accacgccag ccggttatac cgacctgctt gttaactaca cctaccgcat
     7141 cgcttttgaa ggcggcgggg gtcaggactt cggtctggca gcagcaattg ccacgctgat
     7201 cttcctgctg gtaggtgcgc tggcgatagt gaacctgaaa gccacgcgaa tgaagtttga
     7261 ttaagggaga taacaaaaat ggcaatggtc caaccgaaat cgcaaaaagc acgtttgttt
     7321 attactcacc tgctactgct actttttatc gcggcgatta tgttcccgct gctgatggtc
     7381 gtcgccatct cactgcgcca ggggaacttt gcgaccggta gcctgatccc ggagcaaatc
     7441 tcctgggatc actggaaact ggcgttaggt tttagcgttg aacaggctga tggtcgcatt
     7501 acgccaccgc cattcccggt actgctgtgg ctgtggaact cggtaaaggt cgccgggatt
     7561 tccgcgattg gcattgtggc gctctccacc acctgcgcct acgctttcgc ccgtatgcgc
     7621 tttccaggca aagcgacgct gctgaaagga atgctgattt tccagatgtt cccggcagta
     7681 ctttcactgg tcgcgttgta tgcgttgttt gatcgtctgg gtgagtacat tccattcatt
     7741 ggcctgaata ctcacggcgg cgtaattttc gcgtatctgg gtgggattgc gctgcacgtc
     7801 tggaccatca aaggctattt cgaaaccatc gacagttcgc tggaagaagc ggcggcgctg
     7861 gatggtgcga caccgtggca ggccttccgc cttgtcctgt tgccgctgtc agtaccgatt
     7921 ctggcggtgg tgttcatcct gtcgtttatc gctgccatta ctgaagttcc ggtcgcatcg
     7981 ctgttactgc gtgacgtaaa cagctacacc ctggccgtgg ggatgcagca atacctcaac
     8041 ccgcaaaact acctgtgggg tgactttgcc gccgctgccg tgatgtctgc attaccgatc
     8101 accatcgtct tcttgctagc tcaacgctgg ctggtcaacg gcctgacggc aggtggtgtg
     8161 aaaggttaaa gatgttgttc tgccaatgtt atgccgctgc accctcaact tacgttatcc
     8221 caacttgtga ctgttattcg gcgctccacg gagcgccttt ttttctttcg tctgcaatct
     8281 gaatcgttcg ccggttaata tttccatcat agaacttatt atttttacgt tatttgtttt
     8341 cccacttacg ataattctct ttcgtgctct gagtcacggc aatagtattg tttttatcaa
     8401 ttttggataa ttatcacaat taagatcaca gaaaagacat tacgtaaacg cattgtaaaa
     8461 aatgataatt gccttaactg cctgacaatt ccaacatcaa tgcactgata aaagatcaga
     8521 atggtctaag gcaggtctga atgaataccc agtataattc cagttatata ttttcgatta
     8581 ccttagtcgc tacattaggt ggtttattat ttggctacga caccgccgtt atttccggta
     8641 ctgttgagtc actcaatacc gtctttgttg ctccacaaaa cttaagtgaa tccgctgcca
     8701 actccctgtt agggttttgc gtagccagcg ctctgattgg gtgcatcatc ggtggtgccc
     8761 tcggtggtta ttgcagtagc cgcttcggtc gtcgtgattc acttaagatt gctgctgtcc
     8821 tgttttttat ttctggtgta ggttctgcct ggccagaact tggttttacc tctataaacc
     8881 cggacaacac agtgcctgtt tatctggcag gttatgtccc ggaatttgtt atttatcgca
     8941 ttattggcgg tattggcgtt ggtttagcct caatgctctc gcccatgtat attgcggaac
     9001 tggctccagc tcatattcgc gggaaactgg tctcttttaa ccagtttgcg attattttcg
     9061 ggcaactttt agtttactgc gtaaactatt ttattgcccg ttccggtgat gccagctggc
     9121 tgaatactga cggctggcgt tatatgtttg cctcggaatg tatccctgca ctgctgttct
     9181 taatgctgct gtataccgtg ccagaaagtc ctcgctggct gatgtcacgc ggcaagcaag
     9241 aacaggcgga aggtatcctg cgcaaaatta tgggcaacac gcttgcaact caggcagtac
     9301 aggaaattaa acactccctg gatcatggcc gcaaaaccgg tggtcgtctg ctgatgtttg
     9361 gcgtgggcgt gattgtcatc ggcgttatgc tctccatctt ccagcaattt gtcggcatca
     9421 atgtggtgct gtactacgcg ccggaagtgt tcaaaacgct gggggccagc acggatatcg
     9481 cgctgttgca gaccattatt gtcggagtta tcaacctcac cttcaccgtt ctggcaatta
     9541 tgacggtgga taaatttggt cgtaagccac tgcaaattat cggcgcactc ggaatggcaa
     9601 tcggtatgtt tagcctcggt accgcgtttt acactcaggc accgggtatt gtggcgcttc
     9661 tgtcgatgct gttctatgtt gccgcctttg ccatgtcctg gggtccggta tgctgggtac
     9721 tgctgtcgga aatcttcccg aatgctattc gtggtaaagc gctggcaatc gcggtggcgg
     9781 cccagtggct ggcgaactac ttcgtctcct ggaccttccc gatgatggac aaaaactcct
     9841 ggctggtggc ccatttccac aacggtttct cctactggat ttacgggtgt atgggcgttc
     9901 tggcagcact atttatgtgg aaatttgtcc cggaaaccaa aggtaaaacc cttgaggagc
     9961 tggaagccct ctgggaaccg gaaacgaaga aaacacaaca aactgctacg ctgtaatctt
    10021 cctgtccagc acgccgcgcc agttcggcgt gctgtctttt tactcccgct tcagccgttt
    10081 cgaattacac agccacaggg tgatcaccag taacaggatc gcagccgagt agatcaacac
    10141 atccagtggc gatttatgat cgacgatgat caagcgcaca atggcggtga tcccaatata
    10201 gacaaagtaa cgtagcggga agtgaaaacc ggactgaaag tacttcacaa tcagcgcgat
    10261 aaattcgaaa tagagaaagt aaaccaccag tccttctacc agctcatatt tgctggtttg
    10321 ttctggcgcg aatagcacat cagccagatg caccgtttct ttgccgagga agacaaccaa
    10381 aatcaggccg aggcacaaca ggccaagatt gagtacggtc tgcaaaatgg tggagataaa
    10441 ctccacgcgc ggacgagaga gtgacgtcat agcagtacct ccttcacagt ggcgagcttc
    10501 ctgtataacg caaaaatgtg acggagatct atattttttg tgtatgtttt gttcaacctg
    10561 gatttggagc gcgcctccac caaagccgga tatacctacc cgtaggccgg ataagccgcg
    10621 cctggcggcg catccggcaa gacaaaccgc accaaagccg gtaattcaaa aatcacccat
    10681 tcaggtgatt cccagccacc agcaatgcgc gaaaaatcga caatgttcct ctggaacaaa
    10741 tgaaacgtaa cctgataaag gtggtaaaaa tgaagcctta ttttgctgct ttgatgttat
    10801 cagtctctgt tcttcctgct tatgctggtc cgctcggtac tgctgataaa gccgacctac
    10861 cgcaaagcaa tgtctccagc ccaatgatgg cccagtcgct caggcaacca gacctgcaac
    10921 cgatatcgac ggacaggaag acagaatgtt ttcgccttta cacaccagat cgcaaaccgg
    10981 gggtgaactg cgttcctgat ggctcgacag gccattaaga atgaaggtgc cagtgagtgc
    11041 gaaaactggc acctttccca tgacttagcg gaaattgacg ctacggtcgc tggtcatgtc
    11101 atacaactgg aacttacgcc caagttgctg accaccgtca cgcgtcagcg gcgtccagcc
    11161 aattgccgca cggctgcgtg tcgggccaga cgagaagaga tccaacggta cagagacata
    11221 cacgcctttg gtgaagtccc cttcgccgta ctcctctttc gaaacattag tgatcgtggc
    11281 atagccaccc accaccacac cgctatcaaa gcgtttggcg atctccagcg tgccgccttt
    11341 atcccctgcc agatactgcc cgacgctggc tttaactaac acatcctgag cgaaagatgg
    11401 cgtccagtag gcggtcagat gtccggtttt cacgctgtag tcggtgaatt tcatcatatc
    11461 ttttgcacta cgccagtcgc gctgtttaac gtagttggca tccacaccaa acgcccagtt
    11521 gctgtccagc gggcgataca acacttctgc ccccgcaccg ccaaacatgg tttcgagata
    11581 gccgccgtag acctgaccgt agaagccgtt gcccagatgc tggaagtagt tggcttgcag
    11641 gttattcaca tagacatcgt tctgcacata ctcgcgcaca tgggtacgca cgcgcggtaa
    11701 gtgcgagtcc tgcggcggat tggtgtagtt aaacttgtcg tagttgttgg caaggttagc
    11761 gaacaggctg ccggtggtca gcagatggtc cgtcagccac aaatctgccg ttcccatcac
    11821 gccgagctga tacatgtaaa agttttccgg gccaccgacc gactggttca gcaccggatc
    11881 gatatggaaa tcaaagcgtg atttgtcgat ataccagccc tgctcggtgg acttcggaac
    11941 cactggctcg acgcgttttt gcgccagctt cgtttcgtgg cccaacggct ctccggcgag
    12001 atgatttttc aggctggcga catccgtttc cgtcgtcacc tgcggcatgt taaggcgatt
    12061 ttccgtaacg cggatcgtct tgatcccatc cggcagatca ttcatcacga tccgattagc
    12121 acggatgatc ccttcgcgcg aatcacgata tttcacctgc tcgccggtaa catacagcgt
    12181 atcgcctttc gcctggatct gcggatcggc aagtccggca ttgtatttca acagcgttaa
    12241 ctgattcgcc accaccgaat gctgcaaaat ggcatcctgc ggctgcggtt gatattgcgg
    12301 gcgggcgtta tcgttgtaag acgggcgcag atcgttaaag ttggtgcgca gcgtgacgcc
    12361 aaacataaag gtgttgccac gttcatagct aaggttaacg tcggcccaat cggtaacgcg
    12421 ataaatcgcc ccgacgttaa acttactttt ttgctccagc ttcccggcaa aatcctgctg
    12481 ataattattg ccttcatact ccagtttcag gcgcagcggt tgccagggcg tctggtattc
    12541 cacgccgcca aacagtgagg caggaccgtg gaacatctgg ctaccgtcga tagatcccgc
    12601 ctgtttgtag ctgttatcgc gatagcaata tttatcactg gctgaacaga gcggattttt
    12661 cacattaccg ctggtgccca gatatcccca gcccaggccg agcgtaaaat cgaacggccc
    12721 ccaggctttg ctggcaacaa gatattccgc atcaaacagc cccgtaccgc cgatatcccg
    12781 cgcaccaacc gccacttgcg gcagccaata actctcttcc cacaaacgca gtttgagatc
    12841 gaaggcttta tctttatacg tttgatcgcc agagaacgct tcgacgctgc tgtactgccg
    12901 ggtgcgcacg tcggtgtagc gcagcgttgt ttccagccac gggaagagtt gcactgaagc
    12961 tgagtaataa cggtactgat cgttatcacg atagttcaga ctcaactccc cttcccgtgc
    13021 catacgcgcg gtgggcgttt gtaataatcc tacgccaccg aaatccgact gcgacggacc
    13081 aatgggcgca ggatatgttt cgccgtagca agctgtgcta atgcccagcg ccagtaagct
    13141 aagcagatgt ctttttttca ttattgaggt atccgctgcg tcagagtctg aagaatgtcg
    13201 gcgttaaggg catcaggcat ctcactccag acggagtccg cgaggccaac ataaataatg
    13261 ctgccgggca tcggctctac gtgacgcttg ttccagtaag ccaccggcgc tttttgtgtg
    13321 cgtccgtccg ggtaaaccac ccacgcgtag ctacgatccg caccgctaag cagactttgc
    13381 ccggagagat agctcgccac gtcgcgaccg ggagtgaatg gctgattgcc aggacggctg
    13441 ataagcccga acaacgtgac cgtggacggt ggtggtccga cccacagcgt gtagttgccc
    13501 tgcaacggcg ggttagcgcg ttcagcaacg cggacgatat cgggatcaag attgattttt
    13561 tgcctgcccg tcaccttcaa cgcctgaatt tgctggcgta aggcgttaat cgcagcggca
    13621 tcgtcggcgc tggagtctgc ggccagttct gccagccgtg tcagcaacgc ctgctgctga
    13681 cgcaatgctg ccgccgttgc cagctcttcg ctgatcaccg caccgggcca ccagctgttt
    13741 gccagccgcg gttggcctac cagatcgatt aaatgctctg cgcctgttag tgttttggcc
    13801 tcactgctgc cattgctgaa caccttgacg gttcctgccg cgaagaccga tgacgctccc
    13861 acactcaaaa gcaacgcgac aaaagtttgt ttgatcattg ttttgccgcc ttgatgaggg
    13921 tggttttcac cgggaaataa tccgcgccga gatactgttc cgactggcga atttgccctt
    13981 cgctgtcgat ccaatagcgg ttatgccagc gcgcctggtc ggtggagact tcttcgtcca
    14041 gcacgcgaac cggggtttca tcgctgccga ctttgacggt atcggtgcca tcccatttga
    14101 agactgagcg tgcggtggcg tagcgtacct gctggtactc ggtccagccc atcgtgcgcg
    14161 tccatgttgc accatcaaca atttgtgcgg gtttaatcag cgggtcggcg gcaaggttat
    14221 tcacttcaat caggttgtcg ccgccaagca aagtcttcac cagacgacca tgttgtgtga
    14281 cgagagtggc ctgatcctga gtgacccatt tttgttgtcc gtcttcagcg aaggccagca
    14341 ccacaaacaa ctgcggcccg ccattaagct gcatgtactg gctggcgtag ggcatatttt
    14401 gaatatcatc gtccgtcagc tgtacacctg gcgtaccgaa cagactgtcc cacagtgaat
    14461 tgcccagctc tttagtcgtg gctgaacagg cctgtaatag caggcagata agaatgaatg
    14521 caggtcgctt cacgactctt ctccagaggg gcagaataac cacaccggag tgtggttatg
    14581 gtaaatccct tcatacttca agccacagat gcgatgactc gaattatttt ggggatacat
    14641 actttattac tgggtactgg tggtcgtggt tgttgtggtc ccggtattag aaccatcacc
    14701 gccaccggtt gccgcgagcg cgacacccac cgccgagctt acggtgctga cgctggtcgc
    14761 ggaggaacta cccgccgaaa ccgacgttgc tgccgaccct gccgcttcgc ccacctgtac
    14821 aggtgcagcc cacgcagaag tcgccgcaag cgcagatatg gcaaaaatgc catacagaac
    14881 ttttttcata acaatttccc ttcattgaat gaatggagat ttacccaaaa acatttcggg
    14941 cgggatcgag tatacacaac taaagcatgc gaattacggc gcggggaatt agagatgggg
    15001 ttttaggata attggaatga atatcaaata taagctacaa cttatatata aataacatta
    15061 aaaatcatag tgttaaaata aaagtaaaag taattaatct gagtattttc cgaaatcaat
    15121 cacgattaaa aatctatttt aggattaacc tcacggtatg atttccatta aatcacagac
    15181 aaggagattt atctgataaa acaatgccga ctttaagtcg gcattgttat taaggttatt
    15241 taattaatcg tgctatgtat taattgccga atacgatgta catatcggca tcgacctgta
    15301 ggcctgataa gacgcgaccg cgtcgcatca ggcatcggtt gccggatgcg gcgtgaacgc
    15361 cttatccggc ctacatagcg gcatcaggca tgaacgatga ttaaccgcgc cacgctttat
    15421 agcggttaat cagaccattg gtcgagctat cgtggctgct gatttcttta tcatctttca
    15481 gctctggcag aatacggttc gccagctgtt tacccagttc cacgccccac tggtcgaagg
    15541 tgaagatgtt caggatcacg ccctgagtaa agattttgtg ctcatacagc gcaatcaacg
    15601 cacccaggct gaacggagtg atttcacgca gcaggatgga gttggtcggg cggttacctt
    15661 cgaacacttt gaacggcacc acgtagtcaa gcgttgccgg atctttaccc tgatcgcgat
    15721 attcctgctc aaccacttcg cgggatttac caaacgccag cgcttcggtc tgagcaaaga
    15781 agttagacag cagtttctgg tgatgatcag agagcgggtt atgggtgata gccggggcga
    15841 tgaaatcgca cggtaccatt ttggttccct gatggatcag ctggtagaac gcgtgctgac
    15901 cgttagtgcc tggttcaccc cagataatcg ggccagtctg gtaatccaca acgttaccgt
    15961 tacggtcaac atacttaccg ttggactcca tattgccctg ctggaagtac gccgcaaaac
    16021 ggtgcatata ctggtcatac ggcagaatcg cttcagtttc cgcaccaaag aaattgttgt
    16081 accagatgcc aatcagcgcc agcaagacag gcaggttttt ctcggcaggc gtggtggaga
    16141 aatgcttgtc catcgcgtgt gcgccagaaa gcagttcaac gaagttatca aagccgatgg
    16201 agagaacaat cgacaggcca atcgctgacc acaaagagta acgaccgccg acccagtccc
    16261 agaactcgaa catgttggca gtatcaatac caaactcgcc aacggctttg gcattggtgg
    16321 agagcgccgc aaagtgtttc gcaacgtgtt tttcatcacc tgccgctttc aggaaccagt
    16381 cacgcgcgct atgggcgttg gtcatggttt cctgagtggt gaaggtttta gatgctacca
    16441 ggaacagggt agtttccggg tttacttttt tcagcacttc cgcgatgtgg gtgccatcga
    16501 cgttagacac aaagtgcatg ttcaggtggt ttttgtacgg acgcagtgct tcggtcacca
    16561 tgtacgggcc gaggtcagaa ccgccgatcc cgatgttcac tacgtcagtg attgctttgc
    16621 cggtataacc cttccactca ccggagataa tcgcttgtga gaaggttttc atcttctcca
    16681 gcaccgcgtt gacttccggc attacgtctt tgccatcaac cagaatcggg gtattgctac
    16741 ggttacgcag cgctacgtgc agcacggcgc ggttttcagt gcggttgatc ttctcgccag
    16801 agaacatcga cttaatcgcg cccgccagat cgcactcttt cgccaaatct tgtaatttcg
    16861 ccagcgtctc ttcagtgatg cggtttttgg agtaatccac cagcatctga tcgtcgaagg
    16921 ttgcggagaa cttagaaaaa cgatcgccgt ctttagcaaa aagatcggcg atcgtaacgt
    16981 ctttcatttc atcgaagtgt ttctgtagtg cctgccaggc agcggtctgc gttggattga
    17041 tgtttttcat tagcaatact cttctgattt tgagaattgt gactttggaa gattgtagcg
    17101 ccagtcacag aaaaatgtga tggttttagt gccgttagcg taatgttgag tgtaaaccct
    17161 tagcgcagtg aagcatttat tagctgaact actgaccgcc aggagtggat gaaaaatccg
    17221 catgaccccg tcgttgacaa ccgccccgct caccctttat ttataaatgt actacctgcg
    17281 ctagcgcagg ccagaagagg cgcgttgccc aagtaacggt gttggaggag ccagtcctgt
    17341 gataacacct gagggggtgc atcgccgagg tgattgaacg gctggccacg ttcatcatcg
    17401 gctacagggg ctgaatcccc tgggttgtca ccagaagcgt tcgcagtcgg gcgtttcgca
    17461 agtggtggag cacttctggg tgaaaatagt agcgaagtat cgctctgcgc ccacccgtct
    17521 tccgctcttc ccttgtgcca aggctgaaaa tggatcccct gacacgaggt agttatgtct
    17581 gaaattgttg tctccaaatt tggcggtacc agcgtagctg attttgacgc catgaaccgc
    17641 agcgctgata ttgtgctttc tgatgccaac gtacgtttag ttgtcctctc ggcttctgct
    17701 ggtatcacta atctgctggt cgctttagct gaaggactgg aacctggcga gcgattcgaa
    17761 aaactcgacg ctatccgcaa catccagttt gccattctgg aacgtctgcg ttacccgaac
    17821 gttatccgtg aagagattga acgtctgctg gagaacatta ctgttctggc agaagcggcg
    17881 gcgctggcaa cgtctccggc gctgacagat gagctggtca gccacggcga gctgatgtcg
    17941 accctgctgt ttgttgagat cctgcgcgaa cgcgatgttc aggcacagtg gtttgatgta
    18001 cgtaaagtga tgcgtaccaa cgaccgattt ggtcgtgcag agccagatgt agccgcgctg
    18061 gcggaactgg ccgcgctgca gctgctccca cgtctcaatg aaggcttagt gatcacccag
    18121 ggatttatcg gtagcgaaaa taaaggtcgt acaacgacgc ttggccgtgg aggcagcgat
    18181 tatacggcag ccttgctggc ggaggcttta cacgcatctc gtgttgatat ctggaccgac
    18241 gtcccgggca tctacaccac cgatccacgc gtagtttccg cagcaaaacg cattgatgaa
    18301 atcgcgtttg ccgaagcggc agagatggca acttttggtg caaaagtact gcatccggca
    18361 acgttgctac ccgcagtacg cagcgatatc ccggtctttg tcggctccag caaagaccca
    18421 cgcgcaggtg gtacgctggt gtgcaataaa actgaaaatc cgccgctgtt ccgcgcgctg
    18481 gcgcttcgtc gcaatcagac tctgctcact ttgcacagcc tgagtatgct gcattctcgc
    18541 ggtttcctcg cggaagtttt cggcatcctc gcgcggcata atatttcggt agacttaatc
    18601 accacgtcag aagtgagcgt ggcattaacc cttgatacca ccggttcaac ctccactggc
    18661 gatacgttgc tgacgcaatc tctgctgatg gagctttccg cactgtgccg ggtggaggtg
    18721 gaagaaggtc tggcgctggt cgcgttgatt ggcaatgacc tgtcaaaagc ctgcggcgtt
    18781 ggcaaagagg tattcggcgt actggaaccg ttcaacattc gcatgatttg ttatggcgca
    18841 tccagccata acctgtgctt cctggtgccc ggcgaagatg ccgagcaggt ggtgcagaag
    18901 ctgcatttta atttgtttga gtaaatactg tatggcctgg aagctatatt tcgggccgta
    18961 ttgattttct tgtcactatg ctcatcaata aacgagcctg tactctgtta accagcgtct
    19021 ttatcggaga ataattgcct ttaatttttt tatctgcatc tctaattaat tatcgaaaga
    19081 gataaatagt taagagaagg caaaatgaat attatcagtt ctgctcgcaa acgaattccc
    19141 gtacactgaa ctctctggct cactgagggc accatcatgg cactcccccg tattacccaa
    19201 aaagagatga ctgaacgcga gcagcgtgaa cttaaaacac tgctggatcg ggcgcggatt
    19261 gcgcatggtc gcgtactgac caactccgaa accaacagca ttaagaaaga gtacatcgat
    19321 aaattaatgg tcgagcgtga ggctgaagcg aaaaaagccc gccagttaaa gaaaaagcag
    19381 gcttataaac cggatcctga agcgtcattt agctggtcag caaatacgtc gacgcgcggc
    19441 aggcgctgat tagcgcccct tctttttacg ccccggcgag gtaaaacgct taccgttgga
    19501 cgccgggcga ccgccttttt ccgccgtttt ttccatctta acgactggac gtttgatgcc
    19561 cgccgttttc ggtttggcct tcgttttagg ttttgcctcg gaagaggaat tttcgatgag
    19621 cttaaagaga tcgataagct cgtcgtcggt taaatcgcgc cattctccca gcggaatgcc
    19681 gcttaagctg acgttcataa tgcgcgtacg ttccagcttt ttcacttcat agccgaaatg
    19741 ctcgcacatg cgacggatct gacggttcag cccctgcacc agggtaatgc ggaagacaaa
    19801 cggcgcttct tttttgactt tacacttttt agtcactgtc ccgaggattg gcacccccgc
    19861 ccccatacca cgaataaatt catcggtaat cggtttatcg accgtcacca gatactcttt
    19921 ctcatgatcg ttacccgcac ggaggatctt attcaccaga tcgccgtgat tggtgaggaa
    19981 aatcagcccc tgggagtctt tatccaggcg gccaatcggg aacacgcgtt tgctgtggtt
    20041 aacgaaatcg acaatgttat cgcgctcgcc atcttcggtg gtgctgacaa tgcctacggg
    20101 cttgttcagg gcgataagta ccaaatcttc ggcttcccga ggctcaatca actgaccgtt
    20161 tactttcaca acgtcgccgg gtttcacctg atcgccaatg gtggctcgct tgccattaag
    20221 gaacacattg ccttgctcga tatagcgatc cgcttcgcgg cgtgagcaaa ttccgctttc
    20281 gctgatgtat ttatttaaac ggactgatga gtcgggcagc ataaattctc ctgtaaaacg
    20341 cggaatatac cgtacctctt agcggggaaa aacagggtta tgcggcaacg tcgaaattaa
    20401 atgtgatctg ccacgccttt tatcacaaaa gtgtctccgg aagtacaatg caaatttgcg
    20461 tggcaaaata aggataatca acctattacg aatctccgca cacctgtgct gataactact
    20521 acgcagagaa acaaatgtgc aagagaaagc gctatttctc cccatgcaaa gagtgaaaaa
    20581 tcgctttgaa aggccatcat gatggggtta tatcagatca agcggtgaga aagcgtattt
    20641 gcacaaatgt gcagaaccag gaggcggcaa tggaaaacag tgacgatatc cgtttgattg
    20701 tgaagattgc ccaactctat tacgaacagg atatgacgca ggcgcaaatc gcgcgcgaac
    20761 tgggtattta ccgcaccacc atcagccgct tgcttaaacg aggccgcgat cagggaattg
    20821 tcaccatcgc catcaactat gactacaacg aaaatctctg gctggagcag caactgaagc
    20881 aaaagtttgg cctgaaagac gttgtggtgg tgtcgggaaa tgatgaggat gaagagactc
    20941 aactggcgat gatggggtta cacggcgcgc aactgctgga tcgcttgctg gagcctggcg
    21001 atattgtcgg tttttcctgg ggtcgcgcgg tgagcgcact ggttgaaaac ttgccgcagg
    21061 cggggcaatc gcggcagtta atctgcgtac caattattgg cggaccgtcc ggtaaactcg
    21121 aaagccgcta tcacgtaaac acattaacct acagcgcggc tgcgaagctg aaaggggaat
    21181 cgcatctcgc ggattttccg gctctgctgg ataacccctt aattcgtaat gggatcatgc
    21241 agtctcagca ctttaaaacc atctctgcct actgggataa tctggatgtc gccctggtgg
    21301 gaattggctc accggccatt cgcgacggcg ctaactggca tgcgttttat ggtggtgaag
    21361 agagtgacga cctgaatgcc cgccaggttg ctggcgatat ttgctcgcgc ttttttgata
    21421 ttcacggcgc aatggttgaa acgaatatga gcgaaaaaac actctctatc gaaatgaata
    21481 aattaaagca ggcacgatat tccattggca ttgccatgag tgaagaaaaa tacagcggaa
    21541 ttattggtgc actgcgtgga aaatatatta attgtctggt aacgaatagc agcacagctg
    21601 aactattact gaaataaaaa tcttcccgat gtttatcgta ataaaaatta cggcgggtcc
    21661 ctattatcta aaatcaggag tctgttatgc aaacgtggtt aaatttgcag gataaaatca
    21721 ttatcgtcac cggcggcgca tccggtattg gtctggcgat tgtagaggaa ttattagcac
    21781 aaggcgcaaa cgtacagatg gtagatattc acggtggcga tggtcaatat gaagttcata
    21841 aaggctatca gttttggccg accgatattt ccagcgccaa agaggtaaat catacggtag
    21901 cggaaattat ccagcgtttt ggtcgcatcg acggtctggt caataacgcc ggggtcaatt
    21961 tcccgcgtct gctggtcgat gagaaagcgc ctgccgggca gtatgaactc aacgaagctg
    22021 cattcgaaaa aatggtcaat atcaaccaga aaggcgtttt tctgatgtcg caggcggtgg
    22081 cgcgacagat ggtcaaacaa catgatggcg tgattgtgaa tgtttcctca gaaagtggac
    22141 tggaaggctc agaaggccaa agctgttacg ccgctaccaa agccgcgctc aatagcttca
    22201 cgcgctcctg gtcgaaagag ctgggtaaac acggtatccg tgtggtcggt atcgcgcccg
    22261 ggattctgga aaaaaccggg ctacgcacgc cggaatatga agaggcgctg gcgtggacgc
    22321 gcaatatcac cgtcgagcag ctgcgtgaag gctataccaa aaacgccatt cctattgggc
    22381 gcgccggaag attagcagaa gtcgctgatt ttgtttgtta tctgttgtct gaacgcgcca
    22441 gctacatcac cggagtaacc actaacattg caggcggcaa aacgcgcgga taaggaggct
    22501 ttatggtcaa cgccattttt tgtgcccacg gcaaattagc ttgcgccatg ctggaatcgg
    22561 ttcagatggt ttacggcaac accaatgttg aagccgttga atttgtgccc ggtgaaaacg
    22621 ccggagacat cgtagcaaag ctgaaaaagt tagtaagcat tcacagtcaa gatgagtggc
    22681 tgattgccgt ggatttacag tgcggcagcc cgtggaatgc ggcagcgatg cttgcgatgc
    22741 gcaatcctcg tctgcgggta atcagcggac tttctctgcc gctggcgctc gaactggttg
    22801 ataaccagga cagcatgaat gttgatgaac tgtgtgaaca cctgacgcaa atcgcaaagc
    22861 aaacctgcgt tgtctggaaa caactggcaa cgacagagga ggatttctga tgaacattac
    22921 gctcgcccgc attgatgacc gcctgatcca cggtcaggtc actaccgtct ggtcgaaagt
    22981 ggctaacgcc cagcgcatta ttatctgcaa tgacgaagtt tataacgatg aagttcgccg
    23041 gacattattg cgtcaggctg cgccaccggg aatgaaggtt aacgtggtca atattgaaaa
    23101 agccgtcgcg gtttatcaca acccgcaata tcaggacgaa accgtttttt atcttttcac
    23161 tcgcccacag gatgccttag cgatggttcg ccagggggta aaaataggca ccctaaatat
    23221 tggcggcatg gcctggcggc ccggtaaaaa acagttaacc aaagcggttt cattagatga
    23281 tgatgatatt aacgcatttc atgagttaaa taatcttggc gtcatactgg atttacgcgt
    23341 cgtagcgtca gatccctcaa tcaatattat cgacaaaata aacgaacagt taatcgcaaa
    23401 ttaaataata gcgcctgtgt acatatgctt taacaggcag ggatgacgac acttaaaagg
    23461 tgacaaatca tggaaataag taccctacaa attattgcta tatttctttt ttcctgtatt
    23521 gccggaatgg gcagcgtgct ggatgaattc cagactcatc gcccgttaat tgcctgtacg
    23581 gtgattggtt taattctcgg tgatttaaaa accggaataa tactcggtgg tacgctggaa
    23641 ttgatagctc tcggctggat gaacgtcggc gcggcgcaat ctccggattc tgcactcgcc
    23701 agcataatct ccgccattct ggttatcgtt ggtcagcaaa gcatcgccac cggaatcgcc
    23761 atcgcgttgc ctgtggctgc ggcaggccag gtgctgacgg tgtttgcacg taccatcacc
    23821 gtagtgttcc agcacgcggc ggataaagca gcggaagaag cgcggtttcg cactctcgat
    23881 attctgcatg tctccgcgct tggcgtgcag gcgctgcgcg tcgccattcc ggcactgatt
    23941 gtctcgctgt tcgtcagcgc cgatatggtc agcaatatgt taaacgccat tcccgaattt
    24001 gtgacccgag gactacagat tgctggcggt tttatcgtgg tggtcggtta cgccatggtg
    24061 ctacgtatga tgggcgtgaa atatttgatg cctttctttt tcctcggttt cctcgcaggt
    24121 ggctacctcg atctcagtct gctggcgttc ggtggcgtcg gcgtgatcat ggccctggtc
    24181 tacatccagc taaatccaca gtggcgtaaa gctgaacctc aactccaggc caccgcctcc
    24241 accgcccttg accaacttga cgattaacgg agccatcatg gaacagagaa aaattacacg
    24301 cagcgatctg gtgagcatgt ttctgcgctc caacctgcaa caggcgtcct ttaactttga
    24361 acgtattcac gggctgggtt tttgctacga catgatcccc gccatcaagc gactttaccc
    24421 attaaaagag gatcaggttg cggcgctcag gcgacacctg gtgttcttca ataccacgcc
    24481 agccgtatgt ggcccggtca tcggcgtcac tgccgccatg gaagaggcgc gggccaacgg
    24541 cgcggaaatt gatgacggta ccattaacgg catcaaagtc ggtctgatgg gaccattggc
    24601 aggggttggc gatccactgg tctggggaac gctgcgcccg attaccgccg cgctcggcgc
    24661 atctctggca ctttcgggca acattctcgg cccgctgctg ttcttcttta ttttcaacgc
    24721 ggtgcgtctg gcgatgaagt ggtatggcct acagctcggc tttcgcaaag gggtgaatat
    24781 cgtcagcgat atgggcggga atgtgctgca aaaactcacc gaaggcgcgt cgattctcgg
    24841 actgtttgtg atgggcgtgc tggtcaccaa atggacgtca atcaacgtac cgttggtggt
    24901 ttcacaaacg catgccgccg atggctccac cgtcaccatg accgtgcaga acattctcga
    24961 ccaactttgc cctggtttgc tggcgctcgg tctgacgcta ctaatggttc gtctgctcaa
    25021 caaaaaaatt aacccggtat ggctgatttt cgccctgttt ggcttaggga ttatcggcaa
    25081 tgcgctgggc ttcctgtcct gattcttcgc cccggcacga ctgccggggc catcgctcaa
    25141 catgaggtgg tttatgaaaa caacagctct gcgtctttat ggtaaacgtg atttacgcct
    25201 ggaaaccttt gaccttcctg aaatgcagga ggatgaaatc ctcgcgacgg tggtcactga
    25261 cagcctgtgc ctctcttcct ggaaagaggc caacctgggt gaaaaccata aaaaagtacc
    25321 cgacgatgtg gcgaccaacc caatcatcat cggccacgag ttttgcggcg atattctggc
    25381 cgtgggtaaa aagtggcagc acaaattcca gccgggtcag cgttatgtga ttcaggccaa
    25441 cctgcaactc cccgaccgcc cggactgccc cggctactcc ttcccgtggg taggcggcga
    25501 agccacgcat gtggttattc ccaacgaggt catggaacaa gattgcctgc tggcatacga
    25561 cggcgaaacc tattttgaag gctcgatggt tgaaccgctt tcctgcgtga ttggcgcgtt
    25621 caacgccaac tatcatcttc aggaaggtag ttataaccac acgatgggga ttcgcccgca
    25681 agggcgcacg ctgatcctcg gcggcaccgg accaatggga ctgttggcga ttgattatgc
    25741 gctgcatgga cccgttaacc cgtcgctgct cgtcattacc gataccgaca acgataaatt
    25801 gagttatgcg cgcaagcact atccatcaga atcgcaaaca ctgattcatt atctcaatgc
    25861 caccgatgca gcatttgata cgctaatggc gctgagtggc ggtcacggct tcgatgatat
    25921 tttcgtcttt gtgcctaatg aaggactggt gactctcgcc tcttccttgc tggcgacaga
    25981 tggttgcctg aatttcttcg ccggaccgca ggataaacat ttcagcgcgc caattaattt
    26041 ctacgatgtg cattatgcat ttacccacta cgtgggcacg tcaggcggca ataccgacga
    26101 catgcgcgcg gcggtcaaac tgatcgaaga gaaaaaagtg caggccgcaa aagtggtaac
    26161 acatattctt gggctgaatg ccgcgggcga aaccacgctt gaattgcctg ccgtcggcgg
    26221 cggcaaaaag ctggtgtata ccgggaaata cctgccgctg acgtcactca cgcagattca
    26281 ggatcaagca ctggcggcga ttctggcgcg tcatcagggg atctggtccg gtgaggcgga
    26341 gcaatatctg ctcactcatg cagaggcaat ttcccatgat taatcgcgtt actcaactct
    26401 gcatgtcgct ggctggtcgc accagcaact tcgcgaccgg atgtgctgct gattgctgaa
    26461 gcagcacaat ttgcacgaga agcataaaaa atatcagcgc cttaaggggc gctcaaattg
    26521 gtggcaaagt gcacattgtt tatgccggat gcggaatgaa gccttattcg gtctacataa
    26581 ttttgaaaat tcaatatatt gcagttatct ggtaggccag gtaagcgtag cgcatcaggc
    26641 aatgctgtgt ttgtcatcag tctcagcgcc cggtgcaata cgtgacatcg tgagccatgt
    26701 cagtaagtgg ttaaattcgc agtggtagca ttccttccgt gatttcttac taaccggaga
    26761 agtaatggaa ctgcttttat tgagtaactc gacgctgccg ggtaaagcct ggctggaaca
    26821 tgcactgccg ctaattgctg aacagttgca gggtcgccgc tcagcggtgt ttatcccttt
    26881 cgctggcgta acgcagacct gggatgatta cacagcgaaa acggctgcgg ttcttgctcc
    26941 gctgggtgtt tctgtcaccg gtattcatag cgttgtcgat cccgttgccg cgattgaaaa
    27001 tgctgagatc gtgattgtcg gcggcgggaa tactttccag ttgctgaaac agtgccgcga
    27061 gcgcgggctg ctggcaccaa ttactgacgt ggttaaacgt ggcgcactgt atattggttg
    27121 gagcgcaggc gctaaccttg cttgcccaac cattcgtacc accaacgata tgccgattgt
    27181 cgatccgcaa ggtttcgatg cgctgaatct gttcccgctg caaatcaacc cgcacttcac
    27241 caacgcgctg ccggaaggcc ataaaggtga aacccgtgaa cagcgtattc gcgaactgct
    27301 ggtcgtcgcg ccagaactca caattattgg tctaccggaa ggtaactgga tcacagtgag
    27361 taaaggtcac gcaacgctgg gtggcccgaa caccacttat gtgtttaagg ctggtgaaga
    27421 agcggttccg ctagaagctg gtcaccgttt ttaagtgaat tgccggatgc atgatccggc
    27481 atcttgttca tagtgcctga tgcgacgctt gacgcgtctt atcaggccta caggattcac
    27541 aatattaata ctcgtcccgt ccttcatctt catccggctg ttccagcaca ctgtaagcca
    27601 ccgaacaaaa cagcgagttc aggcgctgca tatctcccag taagcctaaa tgtagcgaac
    27661 tggtttcaat gctctgcacg ttttgctgat gcaggcgatc gacgtgggcg tgggaatagc
    27721 ggcgattaag aatacgaaag cgatgcttgc tgcggcgcaa acgacgggcg ctggtaacat
    27781 caccagagaa gaaaaccgac attgccagtt ttaaattact gagcaattgc tcatagagcg
    27841 catccagttc cttcaagcca tcaagcgaaa atgcccgccg tgctgccagc gatttatcgg
    27901 caatttcgct ccccatgcgc tcgacgatat cggaggcctg ttcaaggttg agcgacattt
    27961 cgatgatctc cgcccagcgg cgcgactctt cttctgccag ttcctctttt ggcatccgcg
    28021 ccagatacag cttaatggcg gtatagagaa cgttgatatc atcagccagc ttacgcagct
    28081 ctttctcctg ccgtggctcg ccgtgcatca ctttattcag cccttccatc atctgttcca
    28141 tggcgtcgcc aatacgcagg gtttcgcgcg cggcgttcgc cagagcaagc gtgggcgtat
    28201 ccagcgcgct gacatccaga tgtttgggtc gtagctgggt atccagttcc ggttcatcgc
    28261 gaataatcgt tttgcaaaac cgtgccatcg ggtcaacaaa tggcagcatg gccaggcaac
    28321 gtacaaggtt gtagaagacg tggaaataga tcaccagttc cgcttttggc aatgacagct
    28381 tccccattgt ctctgccagc aaatggacaa acggcaggat aatcaggcta cccaccagct
    28441 taaacagcag actacccagc gcgacacggc gggctgcggc attggcggca ctgttgttga
    28501 gcatcgccag caggccggaa ccgaggttag caccaatcac cagacagagc gccacgggga
    28561 aggagataat gcctgcggcg gtcagagtcg cagtcagcag tacagcagca aggctggagt
    28621 agctgataat ggcgaacatc gcgccaatca gcgcatccag cagaatatcg ccggtcagcg
    28681 aggcaaagat cacctgaacg ccgtttgcct gggtgatcgg cgttacggcc tgcacaatca
    28741 actccagcgc cagcaaaatc agcccaagac caataccgac gcggcccagt tgcccggcgc
    28801 gtgactgttt gcgtccgagg aaaaaaatca cgccgataaa aataagtaac ggtgacagcc
    28861 aggataagtc gaaggtgaga atacgcgcca ttagcgccgt cccgacatct gcacccagca
    28921 caatcaccag agccggtgcg agggctacca gatcctgagc gacgaacgag gtcaccagca
    28981 tggtggtggc attactgctc tgcaccagtg cggtaacgcc gatccccgcg caaaaggcga
    29041 gcggcttctt ttcgacgctc cggctaagga cagtacgcaa acgcgcgccg aagacgcgca
    29101 ttacgccggt tcgaacaata tgagtccccc agaccagcag ggcgacggca gaaagcaggt
    29161 gaagcagcgt taacacgagt tcaggttctc cttatcgtta tatgttcctg aagcaattga
    29221 atgaccaaag gaccggaaac acgctgactg gcatctttac tgtgcctttt gacattcgtt
    29281 tttccggcta agattattcc agcctgtttc cagtataagt gtttcatatt aataaagaga
    29341 cagggcactc attgagcgcc ctgtttgttg taaggaaaag tgacagattt gtgaatcagt
    29401 ccgcgtcata ccccagattc ggtgccagcc agcgctcaac atcggagacg ctcatacctt
    29461 tacggcgggc atagtcttca acctgatcgc gctgaatttg cgccaccgcg tagtacttgc
    29521 tgtccggatg actgaaatac caacccgaaa ccgatgcacc gggccacatg gcgaaagatt
    29581 ctgtgagttt catgccggta tgtttttcca cttccagcag ctcccagatg gtggcttttt
    29641 ccgtatgttc cgggcaggcc gggtagcccg gtgccggacg gatgccctgg tagttttcgc
    29701 ggatcagttc ttcgttgctg aggttctcgt tcggcgcata gccccagtag actttacgca
    29761 cacgctcatg gagatactcc gcaaaggctt cggctaaacg gtcggcaagc gctttcacca
    29821 tgattttgtt gtaatcatcg tgctgcgctt caaaggcatc agccagtgcg tcctcttcca
    29881 gcccgccagt cacggcaaat gcgccgatgt aatctgcttt accagaaagc ttcggcgcaa
    29941 cgaagtcagc gagacagtag ttagcgaatc ctgttttttc ggtctgttga cgcagatggt
    30001 ggctgacgtt gatcacatgg gtgcgcgttt cgtcacggta gatttcaatg tcatcgccca
    30061 cacggtttgc cgggaacagc cccaccacac cacgcggatt cagcgttttc tcggcgctta
    30121 atttatccag catgtcgttg gcgtctttaa acagccgctg cgcctcaacg cctaccactt
    30181 catcttccag aatgcgcgga tacttcccgg ccagcgacca ggtcataaag aacggcgtcc
    30241 agtcgatgta attacgcagc gtttcgatgc tggcttcaac ttcctgcaca ccgagacggt
    30301 gcgccaccgg cggcgtgtaa gcctgccagt caaaagcgaa atcgttatcg cgcgccgttt
    30361 ccagcgtgac cggtggtgtg cgtggtttct tgcgcccgtg ctgaatacgc acggtttcgt
    30421 actccttgcg ggtacgagcg acaaaatcat cgcgctgagt atcggaaagc agcgccgcca
    30481 ccacgccaac ggtgcgcgag gcattctgca catacaccgt cgggccgctg tagttctgct
    30541 caattttcac cgccgtgtgc gcttttgagg tggtcgcgcc gccaatcaat agcggaatgg
    30601 tgaagccctg acgctccatc tctttcgcca cgttaaccat ctcgtccagc gacggcgtga
    30661 taagccccga aaggccaatc aaatcagcat tcacttcttt agcagtacgg agaatttttt
    30721 ccgcaggcac catcacgccg agatcgacaa tttcgtagtt gttacattgc agcaccacgc
    30781 caacgatatt tttaccgata tcgtggacgt cgcccttcac ggtggcgatc accatcttgc
    30841 cgttggtttt gccctgctct ttgctggctt caataaacgg ttcgagatac gccaccgcct
    30901 gtttcatgac gcgcgccgat ttgaccacct gtggcaggaa cattttccct tcgccaaaca
    30961 gatcgccgac cacgttcatg ccgtccatca acgggccttc aatcacttca atcgggcgcg
    31021 tagcctgctg gcgggcttct tcggtatcct gctcgataaa ctcagtaatg cctttgacca
    31081 gcgagtattc cagacgttta ttcacttccc acgagcgcca ctccgcctgc tgggcgttag
    31141 cggtgtcgtc ggttttgctg ccgcgatatt tctcggcaag ctccagtaaa cgctcagtgc
    31201 catcatcgcg acgattaaga atcacatctt ccaccgcgtc gcgcagttca gcgggcaggt
    31261 cgtcgtaaat cgccagttgc ccggcgttga cgatccccat atccatgcca ttgcgaatag
    31321 cgtagtagag gaacaccgcg tgaatagctt cgcgcaccgg atcgttacca cggaacgaga
    31381 acgagacgtt ggagacgccg ccggaaatca gcgcgtgcgg cagttcgcgt ttgatgtctt
    31441 cacacgcgcc gataaagtcc tgcgcatagt tgttgtgctc ttcaatgccg gtggcgaccg
    31501 cgaagatgtt cgggtcgaag atgatatctt ctggcgggaa gccaacctct tcggtgagga
    31561 ttttgtacgc ccgacggcaa atctcgattt tccgtacgcg ggtatcggcc tgtccctgtt
    31621 cgtcaaaggc cattaccacc acggccgcac cgtaacgacg caacaatttc gcgtgatgga
    31681 taaaggcatc gacgccctct ttcatcgaga tagagttaac aatgcctttg ccctggatac
    31741 acttcagacc tttttcaatg acatcccatt ttgaggagtc gatcataatc ggcacgcgag
    31801 cgatatccgg ttcaccggca atcagattga gaaaacgcac catcgccgct tcggcatcga
    31861 gcatcccttc atccatgttg atatcgataa tctgcgcacc gttttccacc tgctggcggg
    31921 ccacatccag cgcctcgctg tatttctctt ctttgatcag gcgcttgaac ttagcggaac
    31981 cggtgacgtt agtccgctcg ccgacgttca caaacaggct gtcgtcgcca atgttcagcg
    32041 gctccaggcc ggacaaacgg caggctacgg gaatttcagg cagtttgcgc ggcgctaatc
    32101 cttctactgc acgactcatc gctgcaatat gttgtggcgt ggtgccacag cagccgccga
    32161 cgatattgag aaaacccgct tgcgcccatt cacgtatctg ttttgccatc gtgtcggcgt
    32221 cgagatcgta ctcaccaaag gcgttgggta gcccggcgtt cgggtgcgcg gtgacgtagc
    32281 attccgcaat ccgtgacagc tcctgcacgt actggcgcag ttcatctggc cccagcgcac
    32341 agttcaggcc aaaggtcagc gcttcggcgt ggcgcaatga gttgtaaaat gcttcggtgg
    32401 tctgcccgga gagcgtgcgc ccggaggcgt cggtgatggt gccggagatc ataatcggca
    32461 gctcaacgcc cagcgcttca aactccgttt tcaccgcaaa aaccgccgct ttggcgttaa
    32521 gggtgtcgaa aacggtttca atcaggatca gatccgcacc accttccacc agcgctttgg
    32581 tggattcgcg ataggccgcc accagctggt caaaagtgat gttgcggaat gccggatcgt
    32641 tgacgtccgg agaaatagac gccgtgcggt tggtcgggcc gagaacaccg gcaacgtagc
    32701 gcggtttctc tggcgtgcgc gcggtccact catcagcaca ggcgcgcgcc agtttcgccg
    32761 ccgcaaagtt gatttccgcc gacagggatt ccatctggta atccgccatc gcaatggtcg
    32821 tggagttgaa ggtgttggtt tcgatgatat ccgcgcccgc ttcaaagtag gcgttgtgga
    32881 tagcggcgat cacttccggt ttgcttagca ccagcagatc gttattgcct ttgaggtcgc
    32941 atggccagtc ggcaaagcgt tcaccacgaa aatcggcttc gttcagtcga tagctctgga
    33001 tcatggtgcc cataccgccg tccagcacca gaatacgttc atttaactgc gcacgcagtt
    33061 gttccacttt gctgctcaca cttgctcccg acacgctcaa cccagattta ataaaaattc
    33121 aacaaaccat actggcataa acgcatctgt ggtaaaagcg accaccacgc aacatgagat
    33181 ttgttcaaca ttaactcatc ggatcagttc agcaactatt gcattagcta acaataaaaa
    33241 tgaaaatgat ttccacgata cagaaaaagg agactgtcat ggtcgcaccc attcccgcga
    33301 aacgcggcag aaaacccgcc gttgccaccg caccagcgac tggacaggtt cagtctttaa
    33361 cgcgtggcct gaaattactg gagtggattg ccgaatccaa tggcagtgtg gcactcacgg
    33421 agctggcgca acaagccggg ttacccaatt ccacgaccca ccgcctgcta accacgatgc
    33481 aacagcaggg tttcgtgcgt caggttggcg aactgggaca ctgggcaatc ggcgcacatg
    33541 cctttatggt cggcagcagc tttctccaga gccgtaattt gttagcaatt gttcacccta
    33601 tcctgcgcaa tctaatggaa gagtctggcg aaacggtcaa tatggcggtg cttgatcaaa
    33661 gcgatcacga agcgattatt atcgaccagg tacagtgtac ccacctgatg cgaatgtccg
    33721 cgcctatcgg cggtaaatta cctatgcacg cttccggtgc gggtaaagcc tttttagccc
    33781 aactgagcga agaacaggtg acgaagctgc tgcaccgcaa agggttacat gcctataccc
    33841 acgcaacgct ggtgtctcct gtgcatttaa aagaagatct cgcccaaacg cgcaaacggg
    33901 gttattcatt tgacgatgag gaacatgcgc tggggctacg ttgccttgca gcgtgtattt
    33961 tcgatgagca ccgtgaaccg tttgccgcaa tttctatttc cggaccgatt tcacgtatta
    34021 ccgatgaccg cgtgaccgag tttggcgcga tggtgattaa agcggcgaag gaagtgacgc
    34081 tggcgtacgg tggaatgcgc tgactttttc tggcgggcag aggcaatatt ctgcccgtca
    34141 tacctgagtg gtaatagaat aagggtgtct gttaatcgca ttgacgccaa aataacttat
    34201 tgtcatacac ttcacacact tttattaact cagcattatt tttaaacatc aaaccactta
    34261 attattaaca aaatcgcaag atctcattcc accgaaactt ttacggctgc tcaatgagat
    34321 tgagcgattt atgatattt
//
LOCUS       DAZFKF010000046        33828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFKF010000046 DAZFKF010000000
VERSION     DAZFKF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.046"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            121..549
                     /gene="uspC"
                     /locus_tag="ACHKA1_002671"
     CDS             121..549
                     /gene="uspC"
                     /locus_tag="ACHKA1_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO5680305.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGN"
     gene            complement(556..1980)
                     /gene="otsA"
                     /locus_tag="ACHKA1_002672"
     CDS             complement(556..1980)
                     /gene="otsA"
                     /locus_tag="ACHKA1_002672"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO5680306.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(1955..2755)
                     /gene="otsB"
                     /locus_tag="ACHKA1_002673"
     CDS             complement(1955..2755)
                     /gene="otsB"
                     /locus_tag="ACHKA1_002673"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO5680307.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2922..3908)
                     /gene="araH"
                     /locus_tag="ACHKA1_002674"
     CDS             complement(2922..3908)
                     /gene="araH"
                     /locus_tag="ACHKA1_002674"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO5680308.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(3923..5437)
                     /gene="araG"
                     /locus_tag="ACHKA1_002675"
     CDS             complement(3923..5437)
                     /gene="araG"
                     /locus_tag="ACHKA1_002675"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO5680309.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5507..6496)
                     /gene="araF"
                     /locus_tag="ACHKA1_002676"
     CDS             complement(5507..6496)
                     /gene="araF"
                     /locus_tag="ACHKA1_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO5680310.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7293..7796
                     /gene="ftnB"
                     /locus_tag="ACHKA1_002677"
     CDS             7293..7796
                     /gene="ftnB"
                     /locus_tag="ACHKA1_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO5680311.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(7875..8126)
                     /gene="yecJ"
                     /locus_tag="ACHKA1_002678"
     CDS             complement(7875..8126)
                     /gene="yecJ"
                     /locus_tag="ACHKA1_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO5680312.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8241..8327)
                     /gene="azuC"
                     /locus_tag="ACHKA1_002679"
     CDS             complement(8241..8327)
                     /gene="azuC"
                     /locus_tag="ACHKA1_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO5680313.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            8590..8913
                     /gene="yecR"
                     /locus_tag="ACHKA1_002680"
     CDS             8590..8913
                     /gene="yecR"
                     /locus_tag="ACHKA1_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO5680314.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9084..9581
                     /gene="ftnA"
                     /locus_tag="ACHKA1_002681"
     CDS             9084..9581
                     /gene="ftnA"
                     /locus_tag="ACHKA1_002681"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO5680315.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(9619..9858)
                     /gene="yecH"
                     /locus_tag="ACHKA1_002682"
     CDS             complement(9619..9858)
                     /gene="yecH"
                     /locus_tag="ACHKA1_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO5680316.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10049..11260
                     /gene="tyrP"
                     /locus_tag="ACHKA1_002683"
     CDS             10049..11260
                     /gene="tyrP"
                     /locus_tag="ACHKA1_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO5680317.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11322..11987)
                     /gene="yecA"
                     /locus_tag="ACHKA1_002684"
     CDS             complement(11322..11987)
                     /gene="yecA"
                     /locus_tag="ACHKA1_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO5680318.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAYPQEQAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12183..12269)
                     /locus_tag="ACHKA1_002685"
     tRNA            complement(12183..12269)
                     /locus_tag="ACHKA1_002685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12233..12235),aa:Leu,seq:taa)
     gene            complement(12282..12355)
                     /locus_tag="ACHKA1_002686"
     tRNA            complement(12282..12355)
                     /locus_tag="ACHKA1_002686"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12321..12323),aa:Cys,seq:gca)
     gene            complement(12410..12485)
                     /locus_tag="ACHKA1_002687"
     tRNA            complement(12410..12485)
                     /locus_tag="ACHKA1_002687"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12450..12452),aa:Gly,seq:gcc)
     gene            complement(12637..13185)
                     /gene="pgsA"
                     /locus_tag="ACHKA1_002688"
     CDS             complement(12637..13185)
                     /gene="pgsA"
                     /locus_tag="ACHKA1_002688"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO5680319.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13242..15074)
                     /gene="uvrC"
                     /locus_tag="ACHKA1_002689"
     CDS             complement(13242..15074)
                     /gene="uvrC"
                     /locus_tag="ACHKA1_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO5680320.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15071..15727)
                     /gene="uvrY"
                     /locus_tag="ACHKA1_002690"
     CDS             complement(15071..15727)
                     /gene="uvrY"
                     /locus_tag="ACHKA1_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO5680321.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16023..16199
                     /gene="yecU"
                     /locus_tag="ACHKA1_002691"
     CDS             16023..16199
                     /gene="yecU"
                     /locus_tag="ACHKA1_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO5680322.1"
                     /translation="MIKIFIGHYINVFYSTVDITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            16186..16410
                     /gene="yecF"
                     /locus_tag="ACHKA1_002692"
     CDS             16186..16410
                     /gene="yecF"
                     /locus_tag="ACHKA1_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO5680323.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(16478..17200)
                     /gene="sdiA"
                     /locus_tag="ACHKA1_002693"
     CDS             complement(16478..17200)
                     /gene="sdiA"
                     /locus_tag="ACHKA1_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO5680324.1"
                     /translation="MQDKDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQLLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(17430..18182)
                     /gene="tcyN"
                     /locus_tag="ACHKA1_002694"
     CDS             complement(17430..18182)
                     /gene="tcyN"
                     /locus_tag="ACHKA1_002694"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO5680325.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGESKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(18179..18847)
                     /gene="tcyL"
                     /locus_tag="ACHKA1_002695"
     CDS             complement(18179..18847)
                     /gene="tcyL"
                     /locus_tag="ACHKA1_002695"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO5680326.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(18862..19848)
                     /gene="dcyD"
                     /locus_tag="ACHKA1_002696"
     CDS             complement(18862..19848)
                     /gene="dcyD"
                     /locus_tag="ACHKA1_002696"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO5680327.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNVLGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(19953..20753)
                     /gene="tcyJ"
                     /locus_tag="ACHKA1_002697"
     CDS             complement(19953..20753)
                     /gene="tcyJ"
                     /locus_tag="ACHKA1_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5680328.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(20841..21392)
                     /gene="fliZ"
                     /locus_tag="ACHKA1_002698"
     CDS             complement(20841..21392)
                     /gene="fliZ"
                     /locus_tag="ACHKA1_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO5680329.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPSSDIY"
     gene            complement(21438..22157)
                     /gene="fliA"
                     /locus_tag="ACHKA1_002699"
     CDS             complement(21438..22157)
                     /gene="fliA"
                     /locus_tag="ACHKA1_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO5680330.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDNNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(22477..24189)
                     /locus_tag="ACHKA1_002700"
     CDS             complement(22477..24189)
                     /locus_tag="ACHKA1_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO5680331.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGETITIDL
                     KKIDSSTLKLTSFNVNGKGAVDNAKATEADLTAAGFSQGAVVSGNSTWTKSTVTTFNA
                     ATATDVLASVSGGSTISGYTGTNNGLGVAASTAYTYNATSKSYSFDATALTNGDGTGA
                     TTKVADVLKAYAANGDNTAQISIGGSAQDVKIASDGTLTDVNGDALYIGSDGNLTKNQ
                     AGGPDAATLDGIFNGANGNAAVDAKITFGSGMTVDFTQASKNVDIKGATVSAEDMDTA
                     LTGQAYTVANGAQSFDVAAGGAVTATTGGATVNIGADGELTTATNKTVTETYHEFANG
                     NILDDDGAALYKAADGSLTTEATGKSEATTDPLKALDDAIASVDKFRSSLGAVQNRLD
                     SAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLS
                     LLQG"
     gene            24455..25861
                     /gene="fliD"
                     /locus_tag="ACHKA1_002701"
     CDS             24455..25861
                     /gene="fliD"
                     /locus_tag="ACHKA1_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO5680332.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            25886..26296
                     /gene="fliS"
                     /locus_tag="ACHKA1_002702"
     CDS             25886..26296
                     /gene="fliS"
                     /locus_tag="ACHKA1_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO5680333.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            26296..26661
                     /gene="fliT"
                     /locus_tag="ACHKA1_002703"
     CDS             26296..26661
                     /gene="fliT"
                     /locus_tag="ACHKA1_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO5680334.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            26739..28226
                     /gene="amyA"
                     /locus_tag="ACHKA1_002704"
     CDS             26739..28226
                     /gene="amyA"
                     /locus_tag="ACHKA1_002704"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO5680335.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVITDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(28260..28673)
                     /gene="yedD"
                     /locus_tag="ACHKA1_002705"
     CDS             complement(28260..28673)
                     /gene="yedD"
                     /locus_tag="ACHKA1_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO5680336.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKAGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            28860..30065
                     /gene="yedE"
                     /locus_tag="ACHKA1_002706"
     CDS             28860..30065
                     /gene="yedE"
                     /locus_tag="ACHKA1_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO5680337.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            30062..30295
                     /gene="yedF"
                     /locus_tag="ACHKA1_002707"
     CDS             30062..30295
                     /gene="yedF"
                     /locus_tag="ACHKA1_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO5680338.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            30404..31072
                     /gene="yedK"
                     /locus_tag="ACHKA1_002708"
     CDS             30404..31072
                     /gene="yedK"
                     /locus_tag="ACHKA1_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO5680339.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            31183..31662
                     /gene="yedL"
                     /locus_tag="ACHKA1_002709"
     CDS             31183..31662
                     /gene="yedL"
                     /locus_tag="ACHKA1_002709"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5680340.1"
                     /translation="MFTIKTDDLTHPAVQTLVAYHISDMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGMGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAQD
                     RCLHRLSLETGTQAGFTACHHIYLKHGFVDCEPFADYQLDPHSRFLSLTLCENNELL"
     gene            complement(31817..32935)
                     /locus_tag="ACHKA1_002710"
     CDS             complement(31817..32935)
                     /locus_tag="ACHKA1_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5503 domain-containing protein"
                     /protein_id="HFO5680341.1"
                     /translation="MFPLNDLSLKTQSVQLNKVTSNTESTIKQQELISDDAMINELSS
                     ELVSCLGNGKFTPISEDSKLLNMLSEFKLLREQCFRWGNYTLLFENYGAYDKTGAITI
                     EKSQGEGTLPIRHKLEFISTNIAELLDKLTKITDARLCKGFSDWASSVKEGASNDVKE
                     NVDRALLRMFKCVELHNNELDLSYLFWGSVPPLPEWIEMLYLSDNQLDSVQVPESCKE
                     LGLDSNNLTEFPQVPDGITLISVNNNLISHIDSFPPKIKEIYIRHNKLSQIPEIPDTT
                     TVFDCGCNKIQEIRYFPKNLEKALIEYNNIEVVPAIHSKLKLLFIECNPIKEAFLMPW
                     TLTDITYGISQRKYIVTNPDDYDKYSYMVKRIVIDGHN"
     gene            33119..33259
                     /locus_tag="ACHKA1_002711"
                     /pseudo
     CDS             33119..33259
                     /locus_tag="ACHKA1_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            33236..33615
                     /locus_tag="ACHKA1_002712"
                     /pseudo
     CDS             33236..33615
                     /locus_tag="ACHKA1_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         8530 a         8562 c         7969 g         8767 t
ORIGIN      
        1 attgtcttta ctaataatca gactaatatt tacctgtttg actgagttgg gattgcgtcg
       61 tttctccatt aggagtaaag ctttaatgtc acctgaagtt cacagaataa ggaacaggtt
      121 atgagctata gcaatattct tgtcgctgtt gcggttacac cggaaagtca gcaactgctg
      181 gcaaaagcgg tatctatcgc cagaccagta aagggacaca tcagtttaat tactctcgct
      241 tccgacccgg aaatgtacaa tcaattagct gcgccgatgc ttgaagattt acgcagcgtg
      301 atgcaggaag aaacgcaaag ctttcttgat aagttaattc aggatgcggg gtatcccgtt
      361 gacaagacgt ttattgccta cggtgagtta agcgaacata ttctggaagt ttgtcgcaaa
      421 tatcatttcg atttggtgat ttgcggtaat cacaatcaca gtttcttttc gcgagcatcc
      481 tgctcggcga aaagagtgat tgcctcaagt gaagtcgatg tgctgttagt tccacttacg
      541 gggaattaac cgctcctacg caagcttagg aaaggtagca actttatcgc gctgctggct
      601 ttccgcgctt cgcggaacta tctgctttag gtcgctgatg aagcactcct gccagtggtt
      661 aatatcgttt ttcacgataa cgtccagcat ttctgcatga cgggaaatac gttccgccag
      721 cgacatagtc aatgcacgat ccagcgcagc tgcaacttcg tcacgatcgt agggattaac
      781 aattaacgcc gacgttaact cgtttgccgc tcccgcaaat tgcgaaagaa caagaacgcc
      841 cggattggct gggtcctgag cggcaacata ctcttttgcc accaggttca tcccgtcacg
      901 cagtggcgtc actaagccca cgtcagagta gcggaatatt ttcatcagta atttacggtc
      961 aaaatgctga ttcaaataat aaagaggcgt ccagcctaat tgcccgtatt taccattaat
     1021 tcgtccagct tcattttcga gctgatgacg aatatcctga taggcttgca catcaccacg
     1081 cgacgttggt gcaatctggg tataacgaat tttaccatga tgctgcggat atttttccag
     1141 caacgcttca taggcgagaa aacgctctgg caaacctttg gaataatcca gccgttcgac
     1201 agaaaagata ttttgtacgt ttttcagttc cgctttaagt tgcgccagtt ttggcggcag
     1261 tggcccggca gcctgtttgg ctatttcttt tggctcaatg ccaatcgggt aaacttctgt
     1321 tcgaaacgct ttgccccagg ctgtatggct ttttgcgcta cgtgtcgtga cgcgggtcag
     1381 gttagaaagg caatccagga acgccagacg atcgttttct gtctggaaac ccagcaaatc
     1441 ataatcacaa agctgttcaa gcaaggtgtc atatgtcggc agcgcgttga agatttccgg
     1501 tgtcgggaaa ggaatatgca gaaagaaacc aatgcgatta ttcactcccc gtttgcgtaa
     1561 ttcatgcgca aatggcaaca ggtgataatc gtggatccag ataatgtcat cgtcttgcaa
     1621 cagcggcagt aatttatctg ccagcaacgc atttacgcgt aaatagccgt cccaggcagg
     1681 acgctgaaat tgcaccagat cgaggcgata atgaaaagca ggccagagaa cggcattgga
     1741 gaattggttg tagtattcgt caaggtcctg ttcgctgagg ttaaaagagg cccacgtaat
     1801 gttacctttt ttcacctttt ttagcggctg atcctcattc cctgtttcac cactccagcc
     1861 aaaccacagt ccgcctgcgg ctttcagtgc ccccagtatg ccaacggcaa ggccaccggc
     1921 actggcggcg tgctcatctg gtggtgcaat ccggttagat actacgacta aacgactcat
     1981 agtcatcact cctgttattt tctctttttt gttgtaatgc ggtggttatc atttcaagcc
     2041 agctccagac atccggtacg cccgccagtc gccatgatgc ctgagttgcg cctgtgccaa
     2101 tttttactga cattccgccc agtcggttaa cgactgcgaa gccagattca tcggttaaat
     2161 catcgcccag aaatacgggc gttcgcccga taaagggagc ttcctgcata aaggcagcta
     2221 tcgcctcacc tttactggta cctctcggtt tgatctcgac aacacacttt ccctgctgta
     2281 acgccatttg tggccagatc tgagtaatac gttgcgctaa tgtcattaat gcgtcttcat
     2341 gctgcggagc ctgacgataa tgcagcgcaa aagccatccc tttcgtctcc agctccgcgc
     2401 cgggatactg ggcgatgacc gtatgcagtt gcacgctaat atcacgcgca atcgcatccg
     2461 gcagatgaac gatatgcgtt ttaccattga tgtcacggcg ctccgcccca tgcacgcccg
     2521 ctaacgggaa gcgataaggt tttgccagtg cgtccagctc caccattgag cgccctgata
     2581 tcaatgccaa tgcaccatca cttgcggttg ccagtagctg aagtccttgc agaatattgt
     2641 caggtacgac gacctgatcg ggatgcggtt tgatttccgc cagcgttcca tcgagatcaa
     2701 aaaaccaggc atatttcgcg gatagttcag gggtttcggt taacggttct gtcacccggt
     2761 tctcctcctt ctcttttctt attgttttgc gcagaacgcg ctttacagac atgtaagtat
     2821 agacagtgtg acgggggtcg ccatttgcaa cgaagaacag ccaaccgttg tggcggctgg
     2881 ctgtggtggg aaaaaacgtt agattgttgt ggaaaaaagc atcagacagt gcgtttcgct
     2941 ttttgcttgt aacggtcgaa gatcaccgct gccagcagga ttaagccgcg aaccacgtac
     3001 tgcgcgaaag gagaaatatt aagcaggttc atggcgtttt ccacggtgcc taaaattaag
     3061 atacccgcca ccacatatga gatttttccg atgccacctt tcagagaaac gccacctaaa
     3121 acgcaggcgg agataacaat aagctcataa ccaatcgacg tcattggctg cccactagtc
     3181 atacgtgaag ccagaataat tccggctatc gcagatacca ggccagagag aacaaagata
     3241 ataattttgg tgcgaacaac cggtacaccc gccagacgcg cagcctcttc gttcccgcca
     3301 atcgccaggg tattacgacc aaaggtggtt ttattcagca gcaaaccaaa gataatcaga
     3361 cacgcgacgg tgagccagat tggcgcaggc agaccgaacc agttagcgta accaagggca
     3421 aagaagcttt catcttcgat accgaccgct ttaccgtcgg aaatgatgta cgccagacct
     3481 cgaacaatct gcatcgttgc cagtgtcgtg atcagagcat ttattttcag tttggcgata
     3541 acaaagccat tgaccaggcc acagagaatg ccaagcagca accccgctgc cacgccaatc
     3601 cacaggcttt cagtcaggtt gataaccacc gccgtggtga cacccgcaca ggcaattacg
     3661 gaggcgacag aaaggtcaaa gtcaccggaa gcaaggcaga acaacatgcc acacgccacc
     3721 atccccgaca tggaaattgc caggcccaac cctttcatat taatgaaggt ggcaaagttc
     3781 gggacaaaaa tggcacaggc gataaaaagc accgcaaaca ccaccagcat gccgtactga
     3841 tcccagatac gcccgaagct gaatgacgac ttaggtgcgc cagaacccga tgtagaaaca
     3901 gaagacatca tactctcctt actcaggcaa ctgcctggct gactttaggc atcgcaaggc
     3961 tcagtgcctg acgctcatct gcctgctcgt gtaacaattc accggcgatt tcaccttccc
     4021 gcatcaccac aatccggtcg gcaacgccga ggacttcagg taagtcgctg gaggcaaaca
     4081 gcaccgccac gccctgcgcc gccagcgcat aaattacgtt atatatttcg tgcttagcgc
     4141 caacatcaat gccgcgcgta ggttcatcca gcaaaatgac cttcatctct tccgataacc
     4201 agcggcccag aatggctttt tgctgatttc cgcctgagag attcatgatc agttgctccg
     4261 cgcccggcgt tttgatgttg agcgaacgaa tgtggtgatc ggcattgttt tcttcccaac
     4321 cgttgttgat tacacaaccg ccgagcacat gtttacgtct ggcactgatg ttgatattgt
     4381 cgcgaacgga gtgcacagga ataatgcctt ccgctttgcg atcttccggg cagagcatca
     4441 tgcctgcggc aatggcgtgg ctcggtttac gaatatcgat cggctgttgg tcgatataaa
     4501 cctgaccggc ggtgatttgc gtcccgccaa acatgccttt cattaattcg ctacgccccg
     4561 cccctaccag accaaacagc ccaacaattt caccactgcg aaccgccaga cttattggcg
     4621 tacgcacgcc tggtgctttc acagcatcaa gacgcaggcg ctcctcgcca taactacgcg
     4681 gttgccagcc gtagatatcg ccaatgtcgc gcccgaccat cgcctgcacc agcgcgtcgt
     4741 ggtcaacctg ctgcatatcg gtaaaggttt tgacataacg tccatcttta aagacagtga
     4801 tggcatcgct gagggcaaat atttcttcca tacggtgaga aacgtataag atcacccgcc
     4861 cctcttttcg cagttcacga ataacgcgga aaagattgtc gatttcgcgg gcagaaaggg
     4921 agctggttgg ctcatcaaag gcgataattt tagcgttacg cgccagcgcc ttggcgattt
     4981 caaccatctg ccactgacca atggagagat atttcagcgg cgtgtccggg tcaatatcca
     5041 taccaagatg tttaagttgt aaacccgcct cataattcag caatgagcga ttcacaatgc
     5101 cgcctttatg cggcagctgg ccgagataga tgttttccgc gacggtcatt tccggcacga
     5161 gatgcagttc ctggtaaata atcgccacac ccgcgttaag tgctgcggtc gtgtcggaaa
     5221 aggacatttc ctgcccatta atcactacag aacccgtggt tggcgcatag ttgccgctga
     5281 ggatttttaa gagagttgat tttcctgcgc cattttcacc catcaacgca tgaacctgac
     5341 cggcatagca gtcaaaacta atatccgtca gcgccttaac gccgggaaac gttttaccga
     5401 tgccgcgaaa tgagagatac ggggtagact gttgcataac gtctccgtga atcactggtc
     5461 gttcacaccc cctctgcatc atgcagaggg gaatttttcc ggcaaattac ttaccgccta
     5521 aacctttttt ctccagttct tctttaaagt tgtcacgcgt gatcagcacc acgtcggtaa
     5581 cttcggtgaa tttggtcggt tcaacacctt ttgctaccca gttgtaaagc atttcgctgg
     5641 acttataacc atgtacgtcc gggcttggca acagggaacc gtagaagccg gttgcctgtg
     5701 ccttagacag ttcgctcacc gcatccacac cgttaatacc gataccgatg atatcggccg
     5761 ctttaaagcc ctgaccttcc gtcgcgcgta cgccgcccag cacggtgctg tcgttcatac
     5821 cgacgatcag ccaatgttta acttccggat gttgaaccag cattgagttg gcagcgtcaa
     5881 atgcccccgg gatgtcgtta gatttggtag gtacctgata aatttgtttt tccgggaatc
     5941 ctgcagcttt cagcgcatcc atagaacccg tagtacggcg acgagccgta tccagttcgt
     6001 tggaggtaat cgccatcacc gcgctttctt tgacatccca gccacgtttc tgcatctctt
     6061 tatacagttc ctggccctga cgttcgccaa ttttggtcgc cgccatcatc accagaggaa
     6121 cggtatccat tggcttacct ttggcgttaa caaactggtc atccacggca atgactttca
     6181 tatcgtagcc acgcgccttc gccacgatgg cagagccgag tttcgggtcc ggagtacaaa
     6241 taacgaaacc ttttgcgcca ctggcggcca ggctatcgat cgcgttcaat gttttttcgc
     6301 catccggcac ggcaatctta ataacctcaa accctaaatc cttcccggct ttatcggcaa
     6361 acttccattc ggtctggaac cacggctctt ccggttgctt caccaggaaa ccgagcttca
     6421 ggttctccgc catagcggat tgtgacataa cggctgccag accaatggct gccagggctt
     6481 tagtaaattt gtgcatggtt ctctccagct ttagtgtcgt tttgtgtagg gcaaaaacga
     6541 atgacattcg ttaaattaat cggaaaacaa agcattacct tttaactaaa agataagtga
     6601 ctgtgttgac atagttttag cgagaaatta attctccata ggagagcaat atcacatcgc
     6661 agaattacag tgagaacgtg cataaattta gcgggaaaag acataaggaa aagccaattt
     6721 gtcagacaaa ttgttgaatg cacagcagat taatccataa gattagcctg gaaatccttg
     6781 ttgtctttgg tacccatgcg ggatgtcttc tttttaacca gtcaataggc cgcattacct
     6841 ggcgttgagt ttttgaaatg gtgtaataac cacaactcaa agatgtggaa aatgcgcgtc
     6901 attcatttcg ccattaatta tcactgtgct cattaattaa cagaacacgt ataatgagag
     6961 ccatctcgca aaaatgaaaa aacgttttat aaaatcatca cttcatcatg aattcaaatt
     7021 catcgattaa tatcaacaag atacaaaaag cactatcatt aaaattcatt gcagttacat
     7081 tgatctcatc aatgaaatgt aaaaatatat aaacttgatg atttaagcat tttcttatac
     7141 ccgttcagac gttattctta tttcagatca tcgtcagaat tgactccacg atcacatttc
     7201 ggaccggcag aaaggaatta ttctgcaaac agtaattatg gtgttttgat ttatcttgca
     7261 cctctccact tctggatata aggatattag gtatggcaac cgctggaatg cttctcaaac
     7321 tcaactctca aatgaaccgc gagttttacg catccaatct ctaccttcac ctgagtaact
     7381 ggtgttctga acagagtctg aacggcaccg ccactttcct tcgcgcccag gcacagagta
     7441 atgtgaccca aatgatgcgc atgtttaact ttatgaagag tgtcggcgct acccccatcg
     7501 ttaaagccat tgatgttccc ggtgaaaaac tgaactctct ggaagaactg ttccaaaaaa
     7561 cgatggaaga atacgagcaa cgttctagta cgttggcaca gttagccgat gaagcgaaag
     7621 aactgaatga tgattcaacc gtcaatttcc tgcgcgatct ggaaaaagaa cagcagcatg
     7681 atggcctgtt gctgcaaacc attcttgatg aagtgcgcag tgcgaaactt gcgggtatgt
     7741 gccctgtgca gaccgaccaa catgttctga atgtcgtgtc gcatcagctg cattgatcat
     7801 acatcggcgc taatgcattg cgccgatgaa gtttttgaga aaccgctgcc tcatcttttt
     7861 gaagcagcgg ttttttaatg ggattcgcct tgtggggtaa acttgagttc aatcagcgcg
     7921 atggcttttt ggattgcccg catggtgacc gggtctgcgg cggcgggatg atgagtaaag
     7981 tcgatattct tcagctggct ggacattttt tcgcgaactt caacgggcgc gattacatcg
     8041 agaacatcca gaatttgttt gataaccaac tggcaagcaa ccacatcaga aaccagttcc
     8101 tgatcggcat tcagcggctg ggacatcgta aactcctgat agcattttga aagccgttat
     8161 agtagcggct tcacatcttc agcgatagtc acatccaccg tcatcaggac acaaaaaaac
     8221 ctgccggagc aggttttttg ttatcggaac atattgcctg gcggtacgtc tttgaacgtc
     8281 ttgcaatagt tattgaacat acttttcagg attttgcgca gtttcatcgc ggcactccga
     8341 ccatttgtta tacaggtgtt attgtctttg cgcttataat atgacaacca tcacaaaaat
     8401 caatctttat gtgatgcaaa tcacataaat acccctttaa tgttataaaa atgataacca
     8461 aaaaacagcc cccctatttc tgacacctac agatggcagg aaatagcgcc tgccaggcgt
     8521 cttttccggc cattgtcgca gcgctgtaac gcgtaaaata gtgctttctc ttactcttct
     8581 ggctggacca tgagacttct gattctgact ctttcattaa taacgctggc ggggtgtacg
     8641 gttactcgcc aggcccatgt gagcgaagtt gacgcggcaa caggtattgt acggttggtt
     8701 tatgaccagg cttttttgca gcatgcccat accgatcgtt atgtcagtcg cgggatcgcc
     8761 gatcgcgcat gccagcagga aggctatacc cacgcgatcc cctttggtca gccagtaggc
     8821 aactgtagcc tttttgcggg ttccttatgt ttgaatacgg aattcacttt gtcatatcag
     8881 tgccatcatt ccgccttccc tgtctttctc taaatatttt aatcagcgag gggatcttcg
     8941 ctgattaaag aaatagcggt aatgcgtttt attcccattc gtatttttaa taattgaagt
     9001 ttatatttta ccttttgcaa ataataaaat aacaaattat agtgacgcca cagcaacaaa
     9061 tataaccttt gtggagcact atcatgctga aaccagaaat gattgaaaaa cttaatgagc
     9121 agatgaacct ggaactgtac tcttcactgc tttatcagca aatgagcgcc tggtgcagct
     9181 atcatacctt cgaaggtgct gccgcgttcc tgcgccgtca cgcccaggaa gagatgacgc
     9241 atatgcagcg tctgtttgat tacctgactg ataccggcaa tttaccgcgt attaataccg
     9301 ttgaatctcc gtttgctgaa tattcctcac ttgatgaatt attccaggaa acctataaac
     9361 acgaacaatt aatcacgcag aaaattaacg aactggctca tgctgcaatg accaatcagg
     9421 actacccaac atttaatttc ctgcaatggt atgtttctga gcagcatgaa gaagagaaac
     9481 tgttcaaatc gattattgat aaattaagcc tggcaggcaa aagcggcgaa ggtctgtatt
     9541 ttatcgacaa agaactctct accctcgaca cacaaaacta atgctctccg gcggcaggtt
     9601 ttctgccgcc gtttcaaatc agtgacggca aatcttactc tgatcggtcg aaaaaccttc
     9661 ttctgaaggt ataaatttgc cttttgctgc cagaaacgct accagctctc ccgctgtcat
     9721 cccttctgcc gagcaagtgt gaaaacgtgc ctgttcacca aaacgcgctt taatcgcagc
     9781 ttccagactg gcatgcgtat attgctcgcc tgattcaatc atcatattta acacttcatg
     9841 accgtgaata gagtccatcg ttcctcctca aaaaaaagcc tagcgtagcg attaccgctt
     9901 atgaagactt tgcgccagcg caggactgaa tgcttttatt gtacatttaa atttacacca
     9961 tatgtaacgt cggtttgacg aagcagccgt tatgccttaa cctgcgccgc agatatcact
    10021 cataaagatc gtcaggacag aagaaagcgt gaaaaacaga accctgggaa gtgtttttat
    10081 cgtggcggga accacaattg gcgcaggcat gctggcaatg ccgctggctg cggccggtgt
    10141 tggttttagc gttactttaa tcttgttgat tgggctttgg gcgttgatgt gctacacggc
    10201 gctattactg ctggaggtgt accagcatgt tccggcagat accggtctgg gcacgctggc
    10261 aaaacgctat ctgggacgct acggtcaatg gctgacgggc ttcagtatga tgttcttaat
    10321 gtatgctctg actgcggcat acatcagcgg tgccggtgaa ttgttggcct ccagcatcag
    10381 cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg ctgttgttca cttttgttgc
    10441 cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta tttaaccgtt ttctgttcag
    10501 cgccaaaatt atttttctgg tggtgatgct ggtattgctg ctaccgcata ttcacaaagt
    10561 gaatctttta accctgccgt tgcaacaggg gctggcactg tctgcaatcc cggtgatttt
    10621 tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc agctatatgg atggcaacat
    10681 tcgtaagcta cgctgggtgt ttataaccgg tagtgcgatc cccctggtgg catatatttt
    10741 ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc tttatgggat tgctggctaa
    10801 tcatgccgga ttaaacgggc tgttacaggc gttacgcgaa atggtggcct ctccgcatgt
    10861 tgagctggca gtgcatttat ttgctgattt agccctcgcc acgtcatttc tcggcgttgc
    10921 gttaggctta tttgattatc tggctgattt gtttcagcgt tcaaataccg tgggtggaag
    10981 attgcaaact ggtgcaatta cctttctgcc gccgttggcg tttgcactgt tttatccacg
    11041 aggatttgtg atggcgctgg gttacgccgg tgtggcgctg gcggtactgg cattgattat
    11101 cccttcgctg ttgacctggc aaagcagaaa gcacaatcct caagcgggtt accgggtcaa
    11161 aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt attgctgtga ttggcgtgca
    11221 atttttgatt gcggcagggt tgttaccaga agtggggtga ttagatagcc tcaaattcct
    11281 tattgggtgc cagaattaac gctgacaccc aatttgactt cttaatgcag gcagcactgc
    11341 ttaaatttct taccactacc gcacgggcaa ggatcgttac gccccggttt ctcttctgct
    11401 ttgatcggtt gctgaacagc ttgttcctgc ggatacgcca tccagtatgc atgtaaatca
    11461 agcgccgcca gtcgaatggc atctacgctc tcttcaaacg cttctggcga catcttctct
    11521 acccgctcga agttttcctc agtaccgtgc agcgctatcg cctccagcgc tggttttaac
    11581 gaatcgggca atgttgacca gtcagaaagt gccacgcccc gcatatagcc aaagcaccac
    11641 tcctcaacaa tcgtcagctc gctgccatca acttctcgca agccgaataa cggctcaaac
    11701 tgctccggga attcgttcag acgctctgcg gtatcggcca tatgttgaaa agccaggttc
    11761 ataaagcgcg tcatctcttt ctctgacgcc cagcgcggca catagtcagc cccaccccac
    11821 acggcaacca gccactgtgc cggttcaatc tcttgcggag aactcaacac cgccgtcaat
    11881 aaaccgtcca gctccgccac atcaaggatg gcgtggtcag tgttgtattt ggtcagaata
    11941 tcgtccagcc attccaactc actttcgttt aacggtcccg ttttcatacg cttttccttg
    12001 tggatctcaa ctcgccagca cctatattac atgccggtcc gtatcagaga tactttttga
    12061 gtggctttgc cggtgattaa aaatcaagga gggtgtaacg acaagttgca ggcacaaaaa
    12121 aaccacccgg aggtggtttc acgacactgc ttattgcttt gattttattc ttatctttcc
    12181 catggtaccc ggagcgggac ttgaacccgc acagcgcgaa cgccgaggga ttttaaatcc
    12241 cttgtgtcta ccgattccac catccgggct cgggaagaaa gtggaggcgc gttccggagt
    12301 cgaaccggac tagacggatt tgcaatccgc tacataaccg ctttgttaac gcgccaaatt
    12361 cttcaggcct ttcagccaga catccgcttg aggccgatgt cttttaaact ggagcgggaa
    12421 acgagactcg aactcgcgac cccgaccttg gcaaggtcgt gctctaccaa ctgagctatt
    12481 cccgcattca tcaagcaatc agttaatcac ttgattttat tatcgtctgg caatcagtgc
    12541 cgccgttcga tgcgttgcat tctacttacc tggcgcgatg agtcaacgat atttttcacc
    12601 acttttgatc gtttgctgaa aattgcgccg aaacgatcac tgatcaagca aatctgcacg
    12661 cgcagcgctc aaatattgca acattgacca cagagtcagt accgcagcca caaagaaaag
    12721 tgcaatgccg gcgtactcaa cccaaatgtt cggacgccac agcagccagg ccaacgccac
    12781 catctgggca gtggttttca ctttcccaat ccaggagaca gccacgctac tgcgtttacc
    12841 caactccgcc atccattcgc gtagcgcaga aataataatt tcacgggcga tcatcgttgc
    12901 cgccggtaag gtcacccacc agctgtggta atgctcggtt accagcacca tggcgatagc
    12961 cacgagaact ttatctgcta cagggtcaag gaaagcacca aaccgggtac tctggttcca
    13021 gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc gcgacgcaga aaatgagcgc
    13081 ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc aatacaaaga atgggataag
    13141 gatgacacgg aacagtgtaa gcaacgtagg gatattaaat tgcataatga cgggtaacta
    13201 tctgttgtca gtaagattac ccctatgttg ctacagagac atcaatgttt caacgaccag
    13261 aagatctttt ctgccagacc ttgcgaaata cccggcactt ttgcaatttc ctcgacgctg
    13321 gcgttacgta aaccttgcaa accgcccata tatttcaaca acatttgccg gcgttttggc
    13381 ccgacgcctt caatggtttc cagggaactg gtatttttga ccttcgcccg ttttttacgg
    13441 tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat gctggataac atgcagcgcg
    13501 ggagaatctg gcggcaaact gaatccctca ccttccggct caaagaacag cgtttccagc
    13561 ccagccttac gatctgctcc tttggcaacg ccaagtagca gcggatgatt tttatcccat
    13621 gagacatcca gttcggcgaa aacatttttc gcctgcgcaa gctggccttt gccgccgtcg
    13681 ataagtatca catccgggat cttactgtcg tcgatggctt taccataacg ccgacgcagc
    13741 acctgattca tcgccgcata atcatcgccc ggcgtgatgc cagtaatgtt atagcgccga
    13801 tactccgcac gcagcgggcc gttagcatca aacaccacac aggaagcgac ggtttgttca
    13861 cccatggtat ggctgatgtc aaagcactcc atccgcttca cttccggcaa tttcaacacg
    13921 ctggcaagtg ctgtaagccg ctggtgaacg gtagattgct gcgaaagttt gctggttaag
    13981 gccgtcgccg cattggtgcg cgcgagtttc aggtaacgcg ccctatcgcc acgaggtttg
    14041 gtttgaacat taatcttgcg tcccgccagt tctgaaaggg aatcggcgag cagcgtttta
    14101 tcgctaagat taaaatcgag caggatctca cccggtaagg tgcgcatctg gctgccttgt
    14161 aaatagaact gtcctacgaa ggtttccacc acctcgctca gttccgtacc gccaggcact
    14221 ttcgggaaat agctgcggct gccaagcact ttgccctgac gaatgaacaa tacgtggaca
    14281 caagccatgc ccgcatcgaa cgccacacca ataacgtcaa ggtcgtcgcc ggtattggaa
    14341 acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt ggtcgcgaat acgtgccgct
    14401 tcttcaaact ccagattctg gctggcagtt tccatacgac taatgagttg cgtaagcacc
    14461 tgatcatctt tgccagacaa aaacaggcgc acatactcga cctgctgagc gtattcttct
    14521 tcactcacca gtccttcaac gcacggtccc agacagcgcc ctatctgata ttgcagacac
    14581 ggacgcgagc gattgcgata aacactattt tcgcactggc gaatggggaa aatcttttgc
    14641 agtagcgcca gtgtttcacg tacggcatag ccattcggga acgggccgaa atattcacct
    14701 ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt gggtatcacc actcaggaag
    14761 ataaaaggat atgatttatc atcgcgtagc aaaacgttgt aacgcggctg atagagtttg
    14821 atgtagttgt gttccagcaa cagcgcttcg gtttctgtat gagtaaccgt tacatcaatt
    14881 tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag cgaggttgct acggaaatag
    14941 ctggaaagcc gttttttcag gtctttcgct ttgccgacat agataaccgt accaccagca
    15001 tcgtacatgc gataaacgcc tggctggctg gttacggttt ttaaaaacgc ttttgcgtca
    15061 aactgatcac tcactgactt gataatgtct ccgcattaca cagaccatgg cgaattgcca
    15121 ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact gaacatacga tagcggtagc
    15181 tgttcaccgt tttcggactg agattgagct gttctgagat ctcattgacc ttctggccct
    15241 tggtgatcat cagcataatc tgcaattcac gttcagacaa actggcaaat gggctttctg
    15301 ttttttctgg ttcgatctgg cttaacgcca tttgttgagc gatgtcagaa gcaatgtaac
    15361 gctgccctga atagacagaa cggatcgcac tcacgacttc ctgcggagcc gcgcctttgc
    15421 tgaggtagcc cgcagcaccg gcctgcatga ctttcgctgg taaagggttt tctgtatgga
    15481 cggtaagcat gatgattttg acatcagctg tggaacgcgc gattttacgc gtcgcctcaa
    15541 gaccgccaat gcccggcata ctcatgtcca ttagcaccac gtcaacggca tttgcccggc
    15601 accacttaac ggcgtcttca ccgcacgatg cctcaccgac gacttttata ccctttatat
    15661 cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg gtcatcaaca agtagaacgt
    15721 tgatcaaagg aatatctcca gaaataggga taacgctact gatagttagt cattcgtata
    15781 ttagcggttt ttattgcaac tttgaaacgt taaaaatggt gggtattcga tttttctctc
    15841 gtttttgtaa attcgattgt ccacagttat ggtcacgccc gtcaccaacg gcttcaggga
    15901 tcgtttcaca gtaagccctg cgatgaaatg tgacaaaaat tgactttatt cagcaaaaat
    15961 gaaaatcagc ccgctgaata tgtaacatta attaacccga acaatacggg caaaaaacat
    16021 tcatgataaa aatatttatt ggtcattata ttaacgtatt ttatagtact gttgatatca
    16081 cgctcaaaaa acaaccactg ctatttttag caaagcttat ggtatactcc gccgccttaa
    16141 catttttcac cgcaaatttt cattgcaaca tgacgaggaa aataaatgag tacgcctgat
    16201 ttttctactg ccgagaataa tcaagaactg gcaaatgaag tctcctgcct gaaagcgatg
    16261 ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg gccgcgtgat gttaaagatg
    16321 gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg cagtattttc caaaacggtt
    16381 aagcaaatta aacaagccta ccgtcagtaa tataaaaccg gctgatagcg tgcctttcag
    16441 ccggtttttg catctggcac gcaggacaga aaagagatca aattaagcca gtagcggccg
    16501 cgtaacaggc aacctgggtc ttatttggtg cattaatttt tttctgcatg tttttctggt
    16561 ggaaattgac cgtattctcc gagattgaca aaatcatcgc tatctctgct gatgtcttcc
    16621 cttccgccgt ccacctcaga atttcttttt cgcgcttgct gaaattcatc tctggcgtca
    16681 tcactatttc atcatttaaa cgcatcagag ccatcagact ttcgcgcacc agtaactgca
    16741 tttttaattg cagttcatca ctaagaatgg gtatttcgcg cgcgctgcaa cgggaaaagg
    16801 acaaaaagcc cagcgcccgg tttggcagca ttaaatactg agtgacaccg cggcgtaaac
    16861 catgtgcgcg cgcggcttcc cataacggct gtgcttcgct gaataagtca tcattccaca
    16921 ttaaatggcc ctgactaaag ttttcagggt tcagcaccgg atcaattgcg agaaagtttt
    16981 ttgcctgata ataactaacc cacgcctcag ggtaattggt gtaaaaagcc actttaggtc
    17041 gcgtgaatgg taccgggtgg cgaacacata acgaatagta atcgtactcc agcagctgag
    17101 cctgaagctc tatttcatgg tagacctctt ctgcggcctc catcctctga aaacgcaaca
    17161 gcatcgtgcg acgccagctg aaaaaatcct tatcctgcat agtaaaccgc aacgcccctg
    17221 agagtgagta tcatttataa tgataattca aactatcaca taaataagat ttatatataa
    17281 tttatattat tcagacaatg aattactttt gcaagccatc gcattctctt atgttattaa
    17341 tgagttatgc tgatttgtta aacagtttta tcacgcttga aatggcgttc cgccccgact
    17401 gtcgaatcgt cggggctgat tttttcttat tattgcagca gaaacttctc gaggaactgg
    17461 cgggtgcgag gctgctcggg atcggcaaat aacgcttttg cggccccctg ctcgactatc
    17521 cgcccctggt ccataaagat cgcccggtcc gcaacatccc gggcaaagct catttcgtgc
    17581 gtcacaatca ccatcgtgcg cttttcctgc gccagctgac ggatggtgtt caggacttcg
    17641 cccaccagct ctggatccag cgctgacgtt ggctcgtcaa acaaaatcac ctcaggacgc
    17701 attgccagcg cacgcgcaat cgcaacacgc tgctgttgac cgccagacaa acgacgtgga
    17761 tagctggttt ctttaccggc cagcccaact ttcgccagta gctcgcgagc gcgcgccgtg
    17821 gcctcttctt tcgattcgcc tttgacgatc accggccctt caataatgtt ctccagcacc
    17881 gtacgatgcg gaaacaaatt aaagttctgg aagacaaacc cgacgtgctg acgcaactgg
    17941 cgaatcagcg atttttgctg acttaatgaa cgtgcagtat caatagtgat atcgccaacg
    18001 gtgatagttc ccgcttccgg ttgttccagc agatttatgc tgcgtagcaa cgtggttttg
    18061 ccggaaccac tcggaccgat aattgccacc acttcgccag gctttacctc aaggtcgata
    18121 ccgtgcagca ccgtctgacc gtgaaatttt ttcaccaggt tcttaacttc aatggcactc
    18181 attttggttc tctctcctgg cgattaagtt gattctcaaa atggttctgc aacgtcgata
    18241 acacagtcgc catgatccag tagatgagcg aagccgccag gtacatggtg aacacctcca
    18301 gcgtgcgcga ggtaatcaac tgcgcttgac ggaacagctc cggcacctgg attgtcgcgg
    18361 ccagcgacgt atcttttacc aggctgataa agctgttcga cagcggcggc aacgccaccc
    18421 gcgccgcctg tggcaaaatc gcgcgacgca tagtctgcca tggcgtcata ccaatactgg
    18481 cagctgcttc ccactgacct ttatcaatag aagaaatggc ggcgcgaagc gtttcggcag
    18541 cataggcggc agtgttgagc gacagaccaa tcattgctga cggaatggga tctaattcga
    18601 taccaaactg cggcaggccg taatagatca taaacagttg tgcgatgagc ggcgtaccac
    18661 gaaaaataga gatataaaaa cgcgccagcc agcgcacggg ccagattggc gacaggcgca
    18721 tcagcgcgag aataaacccg agcagtaagc caaaaaacat gccgccgata ctgagttgca
    18781 gcgtataccc agcccctttc agcaggaacg gcaaggaatc aataaccagt tgtatacttt
    18841 cttgcatgag cgtttccgga gctaaacgtg gggatgatag gcgaacagcg caggcgcgcc
    18901 gccggtatga ataaacagaa tcggcccttc atctttgaag cgtttctgac tgataccgtc
    18961 aatcagaccc gccatcgctt ttccggtata cacaggatca agcagaatgc cttccagccg
    19021 cgccagcaat ttcactgctt ccatgccttc gtcgttcggc acgccgtagc caggtgcaaa
    19081 atagtcatcc cagagtaaaa tttctgctga tgcggtcagc tccagttctt tcgcaatcgc
    19141 ctgttgtagg ttaaccactt tcggcaattg atcggcaacg gaacgcgaca cggtcacgcc
    19201 aatcagttcg ctttcaggca tcaggtgttc cagcccaaca gccagtccgg cgtgagttcc
    19261 ggcactgccc gatgcgacta ccaccgacga aatattaacc gccccttcac actgttgcgc
    19321 gatttccagc gcactctcca cataacctag cgcgccaaga acattagaac cgccaaccgg
    19381 aatgacatat gggcgaaagc cttgtgcttc gactcgcgtc gccagctctt ccagttgggc
    19441 attgggatcg gtcagtgcgt cgcacatttc aatctgggta ttgaacagat ccagcaacaa
    19501 gcgattgccg ttggttaaat agttttctgc ggttgtgcca ataggatttt ccagcagcgc
    19561 cacgcagtgc agaccgagtt tggcagcgac tgcggcagtc tggcgcacat ggttagactg
    19621 gatcgccccg gcagtaatca gcgtatcggc accttcacgc agagcatctg ccgcgagaaa
    19681 ttctagctta cgtaatttat tgccgcccat tgccatgggg gtgacgtcat cccgtttgat
    19741 gaaaatttcc cgtccaagat aatcagaaaa gcgcggcaga tattcgagcg gcgttggcgc
    19801 gccgataaac tccagccgtg gaaaacgggt taaattatgc agtggcataa cagcctccga
    19861 tgtgtgttgt tgtgattttc ttattatgca cgctgaaaac gcgtaaataa aaaaggcgct
    19921 agtgaaagcg cccttttttg tcattatgct gattatttgg tcacatcagc accaaaccat
    19981 ttttcggaaa gagcttgcag agtgccgtct ttttgcattt ccgcaattgc atcattcact
    20041 gctttcagca ggtcttcatt tcctttacgc agcgccacgc cagactcctg acgggagaat
    20101 gcttcaccgg ttactgccag cgtatcgttg gttttcttca ccagatccag cgccgccaga
    20161 cgatcaacga ggatcgcatc gatacgccct acgcgcagat cctgatattt ggtcgggtca
    20221 tcatcatagg tacgcacatc gacgccctga acattctgcc gcagccactc ttcatagttg
    20281 gtgcccagac cgacacccac ttttttgcct ttcagatcag cagccgtttt aatggtgcct
    20341 tcgttacctt ttttcaccag cgcctgaata ccagaaatgg tgtacggggt tgagaaatcg
    20401 tattttttct tgcgctcatc agaaatggtg acctgattaa tcaccacatc aatacgttta
    20461 gagtccagcg acgccagcat accgtcccat ttggtcggtt ttagggacgc ctcaacgcca
    20521 agatgttttg ccagctgttg ggcaaattcc acttcaaaac cggttaattt gccgtcatcc
    20581 ccctgaaaac tgaacggcgg ataagttcct tccagaccta ccagcagcgt gccgcgctct
    20641 ttaactttat taagcagacc ttcatctgca aaacttttaa cgctcatgcc cgcaaccagc
    20701 gccacggcca tcacacccat caatgcctga cgtcccagat gtgctaattt catattcacc
    20761 ccgaatgttg ttatgtctgt ttgcagtgta gagccattgt ttgtaaacac aaaaacaact
    20821 ccgctacatc ttattcttat ttaatatata tcagaagaag gcaggctgga ggatttctgc
    20881 acaagtccgg tacaggtttg gcgctgatag tgttgatact tccgtaacgc gatgcggtaa
    20941 ttgttggtgc tggtggtggg cagccacggg gcgaggattt catcaaggaa accgtcctgc
    21001 agctgatcga gggaaatatt ttgctcgtgt agatgattcc ccaaacggcg cagacgaacg
    21061 acatattcac gcaccgtacc agggctcatt tcagtttgct caaaaagaaa ttgcttaaag
    21121 ccgataatat cgaaaaaatc actctgcgtt ttgcaatgta aatcaccgca aaatcggcac
    21181 aatgccgccc atgatttttg ttccgtttgc cagccatttt catcaagcag cgtgtccagg
    21241 cgggaaatct cgattttatt cactattttg ccgtcgcgaa ctaaggtaat gcgatcgagt
    21301 aatttacggc aatgcgcgca atgggtctgg ctgtgtttaa agtctttaag atagcggctt
    21361 aatggccgtc ttttcaggtg ctgcaccatc attaagaact cctggtagtc aaagttaaag
    21421 tgcggcattt actgacgtta taacttaccc agtttagtgc gtaaccgttt aatagcctgg
    21481 ctgtgtaact gactgacccg cgattccccg acctccagca ctgcaccaat ctctttgaga
    21541 ttcagctctt cctggtaata gagggttaat accagttttt cgcgctccgg caacgtttcg
    21601 atggcttcca tcacccgctg gcgcagatta ttgtccagca gttgttgtag cgggttttct
    21661 cgctgatgat catcagtaac cagttcgatg ctatcgccgt gctcttcgcg ccactcatcg
    21721 taggagaaga gctggctgtt attggtgtcg agcaacattt ggcgataatc ggcaatatcg
    21781 atccctaaac gttccgctac ctcagtttcc gtggcgttgc ggccaagttc ctgctccagt
    21841 tgccctattg cctgtgccac ttcacgcgcg ttgcgtcgca cgctgcgcgg cacccagtca
    21901 cggctgcgaa gttcatccag catagcgcca cggatacgct gcactgcgta agttgtaaat
    21961 gccgttcctt gtagggcgtc atagcgttcg acggcattaa gtaacccaat gccgcccgcc
    22021 tgtagcagat cgtcaagttc cacgctcgcg ggcagtcgaa cctgcaggcg caatgcttcg
    22081 tgacgcacca gcgggacata acgctgccat agcgagtgtt tatccattac accttcagcg
    22141 gtatagagtg aattcacgat aaacagccct gcgttatatg agttatcggc atgattatcc
    22201 gtttctgcag ggtttttaat cggacgatta gtgggtgaaa tgaggggtta tttgggggtt
    22261 accggtaaat tgcggacaga aaaacccccg ccggtggcgg gggagcacgt tgctgacaaa
    22321 ttgcgcttga tgttgccgga tgcggcgtaa acgccttatc cggcctacaa aaatgtgcaa
    22381 attcaataaa ttgcaattca ccgtgtaggc ctgataagcg cagcgcatca ggcaatttgg
    22441 cgttgccgtc agtctcagtt aatcaggtta ggacgattaa ccctgcagca gagacagaac
    22501 ctgctgcggt acctggttgg cttttgccag cacggagtta ccggcctgct ggatgatctg
    22561 cgctttcgac atattggaca cttcggtcgc atagtcggcg tcctgaatac gggactgcgc
    22621 ttcagacagg ttggtagtgg tgttgttcag gttggtgact gcggaatcca gacggttctg
    22681 caccgcaccg agggaagaac ggaatttgtc tacagatgcg atagcatcgt ccagcgcttt
    22741 cagcggatcc gtggtcgctt cggatttacc agtagcttca gtggtcagag aaccgtcagc
    22801 cgctttgtac agagccgcgc cgtcatcatc cagaatattg ccgttagcaa attcgtgata
    22861 agtttctgtg acagtcttgt tggtcgcagt cgtcagttca ccatcagcac caatatttac
    22921 ggtagcgcca cctgtagtag cggttactgc cccaccagcg gcaacgtcaa aagactgtgc
    22981 gccgttagct acggtataag cctgcccagt taacgcagtg tccatatctt cggcggatac
    23041 cgttgcgccc ttaatatcca catttttgct agcctgggtg aaatcaacgg tcatgccgct
    23101 gccgaatgta atcttcgcat caactgctgc attaccattc gcaccgttga aaataccgtc
    23161 caacgttgcc gcatctgggc cgccggcctg gtttttagtc aggttgccgt cagaaccaat
    23221 atataaagca tcaccattga cgtcagtcag ggtgccatcg ctggcaattt taacgtcctg
    23281 agcgcttccg ccgatggaga tctgagccgt attatcaccg tttgctgcat aggctttcag
    23341 cacatcagca actttagtgg tggccccagt accatcgcca ttggtaagtg cggttgcgtc
    23401 aaatgaataa gacttgctgg ttgcgttgta ggtatatgca gtagaagccg ctacgcctaa
    23461 tccattgttt gtaccggtat aaccgctaat agtgctgcct ccgctaacgc ttgccagcac
    23521 gtcggtagct gttgctgcat taaaggtagt aacagtagat ttagtccagg tgctgttgcc
    23581 actgacgact gcaccttggg agaagcccgc agcggtcaga tctgcttcag tggctttagc
    23641 attatcaaca gcgcctttac cgttaacatt gaagctggtc agcttcaatg tagaagagtc
    23701 gatctttttc agatcaatcg tgatggtttc accatcattt gcgccgacct gaattttcat
    23761 cgagccatct ttagacagca cgttcacgcc gttgaactgg gtctgaccgg atacgcggtc
    23821 aatttcgtcc agacgagatt tgatttcgtc ctggatggag tccaggtcag aatcggagtt
    23881 agtaccggta gtggcctgaa ccgtcagttc acggatacgc tgtaagttgt tgttgatttc
    23941 ggacagcgcg ccttcggtgg tctgtgcaac agaaataccg tcgttggcgt tacgtgcagc
    24001 ctgagtcagg cctttaatat tagaggtgaa acggttagca atcgcctgac ctgcggcgtc
    24061 atccttcgcg ctgttaatac gcaagccaga agacagacgc tcgatagaac tcgacagcgc
    24121 agactggttc ttgttgatat tattttgagt gatcagcgag aggctgttgg tattaatgac
    24181 ttgtgccatg attcgttatc ctatattgca agtcgttgat tacgtattgg gtttccaccc
    24241 gtcggctcaa tcgccgtcaa ccctgttatc gtctgtcgta aaacaacctt tagaattttt
    24301 ttcacaaaca gccatttttt gttagtcgcc gaaatactct tttctctgcc ccttattccc
    24361 gctattaaaa aaaacaatta aacgtaaact ttgcgcaatt caggccgata accccggtat
    24421 tcgttttacg tgtcgaaaga taaaaggaaa tcgcatggca agtatttcat cgctgggagt
    24481 cgggtcaggt ctggatttaa gttcaatcct cgatagcctc accgccgcgc aaaaagcgac
    24541 gctaacccct atttcaaatc agcaatcgtc gtttaccgct aaacttagcg cctacggtac
    24601 gctgaaaagc gcgctgacga ctttccagac cgccaatact gcattgtcta aagccgatct
    24661 tttttccgcc accagcacta ccagcagcac caccgcgttc agtgccacca ccgcgggtaa
    24721 cgccatcgcc gggaaataca ccatcagcgt cacccatctg gcgcaggcgc aaaccctgac
    24781 cacgcgcacc accagagatg atacgaaaac ggcgatcgcc accagcgaca gtaaactcac
    24841 cattcaacaa ggcggcgaca aagatccgat taccattgat atcagcgcgg ctaactcgtc
    24901 gttaagcggg atccgtgatg ccatcaacaa cgcaaaagcg ggcgtaagtg cgagcatcat
    24961 taacgtgggt aacggtgaat atcgtctgtc agtcacatca aatgacaccg gccttgacaa
    25021 tgcgatgaca ctctcggtca gcggtgatga tgcgctacaa agttttatgg gctatgacgc
    25081 cagtgccagc agcaacggca tggaagtctc ggtcgccgcc cagaatgcgc agctgacggt
    25141 caacaacgtc gccattgaga acagcagcaa caccatcagc gacgcgctgg aaaacatcac
    25201 cctgaacctg aacgatgtca ccacgggcaa ccaaacgctg accatcactc aggacacctc
    25261 caaagcgcaa acggcgatta aagactgggt gaatgcctat aactcgctaa tagatacctt
    25321 cagcagcctg accaaataca ccgccgtaga tgcgggagct gatagccaga gttctagcaa
    25381 tggcgcactg ctcggcgact ccacgctgcg gacgattcag acgcagttga aatcgatgct
    25441 gagtaatacc gtcagttctt ccaactataa aacgctggcg cagattggta tcacgaccga
    25501 tcccagcgat ggcaaactgg aactggatgc cgacaaactc accgctgcac tgaaaaaaga
    25561 tgccagcggc gtaggtgcat tgattgttgg cgatggtaaa aaaaccggca tcacgaccac
    25621 catcggcagc aacctgacca gttggctttc gacaacgggc attattaaag ccgctaccga
    25681 tggcgttagt aagaccctga ataaattaac taaagactac aacgccgcca gcgatcgcat
    25741 tgatgcgcag gtcgcgcgct acaaagaaca atttacccaa ctggacgttt taatgacctc
    25801 gttaaacagc acaagcagct acttaacgca gcagttcgaa aacaacagta attccaagta
    25861 atcattattc atcgggagac aggtcatgta cgcggcaaaa ggcacccagg cctatgcaca
    25921 aattggcgtc gaaagcgccg taatgagcgc cagccagcag cagctggtca ccatgctatt
    25981 tgatggagtg ctgagcgcac tggttagagc gcgcctgttt atgcaggaca acaatcagca
    26041 aggcaaaggc gtctctttgt caaaagcgat caacatcatt gagaacggac tgcgggtgag
    26101 tcttgatgaa gagagcaaag acgaattaac ccaaaacttg attgctcttt atagctatat
    26161 ggtcaggcgc ttgctgcaag ccaatttacg caacgatgtc tccgcagtcg aagaagtgga
    26221 agcattaatg cgtaatattg ccgatgcctg gaaagagtct ttactctccc cttctttgat
    26281 tcaggaccca gtctgatgaa ccatgcaccg catttgtatt tcgcctggca acaactcgtc
    26341 gaaaaaagcc agctcatgtt acgcctggca acggaagaac aatgggacga actcatcgcc
    26401 agcgaaatgg cgtatgtgaa tgcggtgcag gagattgcac atttgactga agaggttgac
    26461 ccgtccacca cgatgcagga gcagctccgc ccgatgctgc gcctgattct cgacaacgaa
    26521 agcaaggtaa agcagttatt acagattcga atggatgaac tggcgaaact ggtcggtcag
    26581 tcatcggtgc aaaaatcggt gttaagtgcc tatggcgatc agggcggctt tgtgctggct
    26641 ccgcaggata acctcttttg aatctgaatg agtcgatggc tcgcgaataa tccgattacg
    26701 gctacgcttc taatgttccc cttgaatgga gtcgaagaat gcgtaatccc acgctgttac
    26761 aatgttttca ctggtattac ccggaaggcg gtaagctctg gcctgaactg gccgagcgcg
    26821 ccgacggttt taatgatatt ggtatcaata tggtctggtt gccgcccgcc tataaaggcg
    26881 catcgggcgg gtattcggtc ggctacgact cctatgattt atttgattta ggcgagtttg
    26941 atcagaaagg cagcatccct actaaatatg gcgataaagc acaactgctg gccgccattg
    27001 acgccctgaa acgtaatgac attgcggtgc tgttggacgt ggtagtcaac cacaaaatgg
    27061 gcgcggatga aaaagaagct attcgcgtgc agcgtgtaaa tgctgacgac cgtacgcaaa
    27121 ttgatgaaga aatcattgag tgcgaaggct ggacgcgtta caccttcccc gcccgtgccg
    27181 ggcaatactc acagtttatc tgggatttca aatgttttag cggtatcgac catatcgaaa
    27241 accctgatga agatggcata tttaaaattg ttaacgacta caccggcgaa ggctggaacg
    27301 atcaggttga tgatgaactc ggtaatttcg attatctgat gggtgagaat atcgatttcc
    27361 gcaatcatgc cgtgacggaa gagattaaat actgggcgcg ctgggtgatg gaacaaacgc
    27421 aatgcgacgg ttttcgtctt gatgcggtca aacatattcc ggcgtggttt tataaagagt
    27481 ggatcgaaca cgtacaggaa gttgcgccaa agccgctatt tattgtggct gaatactggt
    27541 cgcatgaagt cgataaactg caaacgtata ttgatcaggt ggaaggcaaa accatgctgt
    27601 tcgatgcacc gctgcagatg aaattccatg aagcatcgcg catggggcgc gactacgaca
    27661 tgacgcagat tttcacgggt acattagtgg aagccgatcc tttccacgcc gtaaccctcg
    27721 ttgccaacca cgacacccaa ccgttgcaag ccctcgaagc accggtcgaa ccgtggttta
    27781 aaccgctggc gtatgctcta attttgttgc gggaaaatgg cgttccttcg gtattctatc
    27841 cggacctcta cggtgcgcat tacgaagatg tcggtggtga cgggcaaacc tatccgatag
    27901 atatgccaat aatcgaacag cttgatgagt taattctcgc ccgtcagcgt ttcgcccacg
    27961 gtgtacagac attatttttc gaccatccga actgcattgc ctttagccgc agtggcactg
    28021 acgaatatcc cggctgcgtg gtggtcatgt cgaacgggga tgatggcgaa aaaaccattc
    28081 atctgggaga gaattacggc aataaaacct ggcgtgattt tctggggaac cggcaagaga
    28141 gcgtaataac cgacgaaaac ggcgaagcaa ccttcttttg caacggcggc agtgtcagcg
    28201 tgtgggttat cgaagaggtg atttaaattc atccccggcg gcaagccggg gagatttcat
    28261 tacggcagtg gcgtcggtaa cggcgcttta tccagcgcgg cggcacactc tgccgtcggg
    28321 cgatccacac gctccatcgt cataccatcg tactcaatgg tgttaccgtc acgctcaacc
    28381 tcgtacagct cacgttttac ggtaacgtta gtgagatcat cagacatcaa tgtcaactta
    28441 cccggcacgg caatcacccg ctgccactga cggcaatcca gagtatcgcc cgctttagtg
    28501 acaatcaggc tgcctatcgc ttccggactg accagcgcac gttgcggccc tttggtctgc
    28561 cagtagcccg ccagccagtc cggcgcgggg gttttcacga cattgttata gttttcgact
    28621 tcggcacagc ccgccagcaa catgagcgcg cctgcaattg cgagtttttt catcattcgt
    28681 cctgcatgag aagaaaagag gattgtggca ttaaagccct gatgtcgcca gcctttccag
    28741 gttcagagag aaatttgatc taactattat ttttgtgaat tattaccccg aaaataaatt
    28801 tgcatggtgt atgattcgcg actcttgttt caaaccttct gagttcagag gctacattca
    28861 tgtcatggca gcaattcaaa cacgcctggt tgattaaatt ctgggcgccc atccccgcgg
    28921 tcatcgcggc gggtattctc tccacttact attttggcat cactggcacc ttttgggctg
    28981 tcacgggtga atttacccgt tggggcggtc agctcctgca gctgttcggc gtccatgctg
    29041 aagagtgggg ttattttaaa attatccatc tggaaggatc gccattaacc cgcatcgacg
    29101 ggatgatgat cctcggtatg tttggcggct gctttgccgc agcgctgtgg gccaacaatg
    29161 tcaaactacg catgccgcgt agccgtatcc gcattatgca ggccatcatt ggcggcatta
    29221 tcgccggttt tggcgcgcgt ctggcaatgg gctgtaacct ggcggcgttc tttaccggta
    29281 ttcctcagtt ctcgctgcat gcctggttct ttgccatcgc cactgccatt ggttcatggt
    29341 ttggcgcgcg ctttaccctg ctgcccatct tccgtattcc cgtgaaaatg cagaaagttt
    29401 ctgccgcctc accgctgacg caaaaaccgg atcaggcgcg acgtcgtttt cgtctcggga
    29461 tgctggtctt tttcggcatg ctgggctggg cgctgctcac ggcgatgaac caacccaaac
    29521 tggggctggc aatgctgttt ggcgtcggct ttggtttact gattgaacgt gcgcaaatct
    29581 gctttacttc ggcgttccgc gatatgtgga tcaccggacg tacccatatg gcgaaagcaa
    29641 tcattatcgg tatggcggtg agtgccatcg ggatcttcag ttacgtacag ttaggcgttg
    29701 aacccaaaat catgtgggcg ggaccaaacg cggtaattgg tggtttactg tttggttttg
    29761 gcatcgtgct ggctggcggc tgcgaaaccg gctggatgta ccgcgcggta gaaggccagg
    29821 tgcactactg gtgggtcggt ctgggcaatg tgatcggctc aacgattctg gcgtattact
    29881 gggatgattt cgctccggcg ctggccaccg actgggacaa aatcaacctg ctgaaaacct
    29941 ttggcccgat gggtggcctg ctggtgacat atttgctgtt gtttgctgcg ctgatgttga
    30001 ttatcggctg ggaaaaacgc ttcttccgcc gtgcggcacc gcagactgct aaggagatcg
    30061 catgaaaaat atcgttcctg attaccgtct tgatatggtg ggtgaaccct gcccttatcc
    30121 ggcagtcgca actcttgagg cgatgccgca gttaaaaaaa ggggaaatcc tggaagtggt
    30181 gagcgactgt ccgcagtcga tcaataatat tccactggat gcgcgtaatc acggctatac
    30241 ggtgctggat attcagcaag acgggccgac cattcgttat ttaattcaga agtaatccct
    30301 cattcccgct gtgacccaca gcgggaatat cgttgcctct gtaccacata gattttaccc
    30361 gtcccctctc ctattctttt cagtgtgctc tcaggaggtt tctatgtgtg gtcgctttgc
    30421 ccaatcccaa acgcgtgaag attaccttgc gcttctcgct gaagatattg aacgcgatat
    30481 tccctatgat cccgaaccca ttggcagata caacgtcgcg ccgggaacca aagtccttct
    30541 gctcagtgag cgtgatgaac accttcatct ggatccggtt ttctggggat atgctcccgg
    30601 atggtgggat aaaccgccgc tgattaacgc ccgcgtagaa actgcggcaa ccagtcgtat
    30661 gtttaaaccg ctctggcaac atggtcgggc aatctgtttt gccgatggct ggtttgagtg
    30721 gaaaaaagaa ggcgacaaaa agcagcctta ttttatctat cgtgctgatg gacaacctgt
    30781 ttttatggca gcgataggca gcacaccgtt cgagcgcggt gacgaagccg aaggattttt
    30841 gattgtcact gcagcagcag accagggtct ggtagatatt catgaccgcc gcccgctggt
    30901 tctgtcgcca gaagctgcgc gggaatggat gcggcaagag attagcggta aagaagcctc
    30961 agaaatcgcg gccagtggct gtgttccagc aaaccagttc tcctggcacc cggtatcgcg
    31021 cgcggtgggt aatgttaaaa accagggtgc ggagttaatt caacctgttt gataagcctg
    31081 gagattattg atcaacaata ctgcgtcata agtaatctct attagacaaa gatttcatta
    31141 cctgttggca tattgcaaaa ataacaccaa tacggaatcg tcatgttcac gattaaaaca
    31201 gatgatctca cccatccagc agtgcaaaca ttagtggctt accatatttc cgacatgctg
    31261 cagcagtctc cccccgaaag cagtcatgct ttagatgtgc aaaaattacg taacccgaca
    31321 gtgacattct ggtcagtatg ggaaggcgaa caactcgcag gaatgggtgc gctgaagttg
    31381 ctggatgata aacatggcga gctaaaatca atgcgtaccg cgccaaatta tttacgtcgt
    31441 ggtgtcgcca gtctgatttt acgccacatt ttgcaggtcg cccaggacag atgccttcat
    31501 cgcctgagct tagaaacggg tacacaggct ggatttacgg cctgccatca tatttatttg
    31561 aagcatggtt tcgttgattg cgaaccgttt gctgattatc aacttgatcc acacagtcga
    31621 tttttatcat tgacgctatg cgaaaataat gagttgctat gagccagacg cagcacattc
    31681 ttgcattcga cgtgctgcgc ctttatttat caccagcagg aaacgccttg tccatagacg
    31741 ccacttctac atgcgccaca agaaacctct attccagtga cacaattacg cctaattaat
    31801 tacataaatg aattatttaa ttatgaccat ctattactat ccttttaacc atgtaagaat
    31861 atttatcata atcatcggga ttcgtaacaa tatattttct ctgcgatatt ccataggtaa
    31921 tgtctgtcaa agtccatggc attaaaaatg cctctttaat aggattgcat tctataaaaa
    31981 gtaatttcaa cttgctatgt atcgcaggaa caacctcaat gttattatac tcgataagtg
    32041 ctttctccag atttttgggg aagtatcgaa tttcttgtat cttattacag ccacaatcaa
    32101 aaaccgttgt agtatctggt atttctggta tttgcgatag cttattgtgc cgaatataaa
    32161 tttccttaat cttcggcgga aatgagtcaa tatgcgatat caggttatta tttacggaga
    32221 tcagggtaat tccatcaggt acttgtggaa attctgtaag gttattggaa tcgagtccta
    32281 attctttgca tgattcgggc acctgtactg agtcaagttg attatcacta aggtaaagca
    32341 tttcaatcca ctctggaaga ggcggtacag aaccccaaaa taggtatgat aagtcaagtt
    32401 cattattgtg tagctcaaca catttaaaca ttctcagcaa tgccctatcc acattttctt
    32461 tgacgtcatt cgatgcgcct tctttgactg aactagccca gtcactgaaa cctttgcaaa
    32521 gcctggcatc tgtaattttg gttaacttgt ccagcaactc tgcaatattg gtcgatataa
    32581 actctaattt atgtcgaatg ggtaaagtcc cctccccctg acttttttct atcgtgatag
    32641 cccccgtctt atcataagcc ccataatttt caaacaatag agtataatta ccccacctga
    32701 aacattgctc tctcaataac ttaaattctg acagcatatt caataatttg ctgtcttcac
    32761 taattggtgt aaacttacca tttccaagac aactgactaa ctcacttgat aattcattta
    32821 tcattgcatc atcagatatt aactcttgtt gttttatcgt agactcagta ttagatgtga
    32881 ctttatttaa ttgaaccgac tgagttttta acgaaagatc atttaatgga aacatttaca
    32941 ctcctgttta ataaattata ttgcccaggg cgcattctaa aacacaatga aattaccact
    33001 atcaaaaatc gctcatatcc atagcccggg atatgaataa aaaaatcaac aaagattaaa
    33061 ttattcaaac gttactgttg cgtctgtggt cgtaacaaca tccgacgtgg tgatgtgtca
    33121 aatgagacac tctgatgatc agagggttta tcgtgtatac ggatcgtgga tggcagaaaa
    33181 cgttcaggat caggtatcca ttttaaacca gataatgagt aagtttaccc catccatgcc
    33241 ccacgcagtg agatcggatg tcattaaaaa agtcaggtca gcaggtcagt atgggaaggc
    33301 gaacaactcg caggaatggg tgcgctgaag ttgctggatg ataaacatgg cgaactgaaa
    33361 tcaatgcgta ccgcgccaaa ttatttacgt catggtgtcg ccggtcggat tttacgccac
    33421 attttgcagg tcgcccatga cagatgcctt catcgcctga gtttagaaac gggtacacag
    33481 gctggattta cggcctgcca tcaactttat ttgaagcatg gtttcgctga ttgcgaaccg
    33541 tttgctgatt atcgacttga tccacacagt cgatttttat cattgacgct atgcgaaaat
    33601 aatgaattgc catgagccag acgcagcaca ttcttgcatt cgacgtgctg cgcctttatt
    33661 tatcaccagc aggaaacgcc ttgtccatag acgccccttc cacatgcgcc acaagaaacc
    33721 tctattccag tgacacaatt gcgcctaatt aattacatat aatatttaat tatgagttcc
    33781 tcaccatcta ttacatactt tttaaccata tcggaatatt tatcataa
//
LOCUS       DAZFKF010000047        33747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFKF010000047 DAZFKF010000000
VERSION     DAZFKF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.047"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..2654
                     /locus_tag="ACHKA1_002713"
                     /pseudo
     CDS             1..2654
                     /locus_tag="ACHKA1_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta strand repeat-containing protein"
     gene            5547..6407
                     /gene="pdxI"
                     /locus_tag="ACHKA1_002714"
     CDS             5547..6407
                     /gene="pdxI"
                     /locus_tag="ACHKA1_002714"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO5680342.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDILDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            6471..8777
                     /gene="ydbD"
                     /locus_tag="ACHKA1_002715"
     CDS             6471..8777
                     /gene="ydbD"
                     /locus_tag="ACHKA1_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO5680343.1"
                     /translation="MFKPRICSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALKIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIASMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWGFLESALISWDNLPAVVLKELQQNTSR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            8948..9553
                     /gene="ynbA"
                     /locus_tag="ACHKA1_002716"
     CDS             8948..9553
                     /gene="ynbA"
                     /locus_tag="ACHKA1_002716"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO5680344.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYVGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            9553..10449
                     /gene="ynbB"
                     /locus_tag="ACHKA1_002717"
     CDS             9553..10449
                     /gene="ynbB"
                     /locus_tag="ACHKA1_002717"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO5680345.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLAGVLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNTQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMV
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            10465..12222
                     /gene="ynbC"
                     /locus_tag="ACHKA1_002718"
     CDS             10465..12222
                     /gene="ynbC"
                     /locus_tag="ACHKA1_002718"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO5680346.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLIATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPGNEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            12236..13528
                     /gene="ynbD"
                     /locus_tag="ACHKA1_002719"
     CDS             12236..13528
                     /gene="ynbD"
                     /locus_tag="ACHKA1_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO5680347.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGSLNQFTAVQDFNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATIMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIKIALPYVVG
                     ACSCIVLMELMIMLQLWWSVWLCWPVLSLFIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCLRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVNEAISYIRARRPQIVLTDEHKAMLRLWENR"
     gene            complement(13582..14187)
                     /gene="azoR"
                     /locus_tag="ACHKA1_002720"
     CDS             complement(13582..14187)
                     /gene="azoR"
                     /locus_tag="ACHKA1_002720"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO5680348.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            14388..18290
                     /gene="hrpA"
                     /locus_tag="ACHKA1_002721"
     CDS             14388..18290
                     /gene="hrpA"
                     /locus_tag="ACHKA1_002721"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO5680349.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            18562..19362
                     /gene="ydcF"
                     /locus_tag="ACHKA1_002722"
     CDS             18562..19362
                     /gene="ydcF"
                     /locus_tag="ACHKA1_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO5680350.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTIFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPHVPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            19559..20998
                     /gene="aldA"
                     /locus_tag="ACHKA1_002723"
     CDS             19559..20998
                     /gene="aldA"
                     /locus_tag="ACHKA1_002723"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO5680351.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISSGIRECASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(21040..22041)
                     /gene="gap"
                     /locus_tag="ACHKA1_002724"
     CDS             complement(21040..22041)
                     /gene="gap"
                     /locus_tag="ACHKA1_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO5680352.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVNGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            22230..22760
                     /gene="cybB"
                     /locus_tag="ACHKA1_002725"
     CDS             22230..22760
                     /gene="cybB"
                     /locus_tag="ACHKA1_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO5680353.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            23005..23178
                     /locus_tag="ACHKA1_002726"
     CDS             23005..23178
                     /locus_tag="ACHKA1_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680354.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            23204..23272
                     /gene="yncP"
                     /locus_tag="ACHKA1_002727"
     CDS             23204..23272
                     /gene="yncP"
                     /locus_tag="ACHKA1_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO5680355.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(23250..23399)
                     /gene="hokB"
                     /locus_tag="ACHKA1_002728"
     CDS             complement(23250..23399)
                     /gene="hokB"
                     /locus_tag="ACHKA1_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO5680356.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            23798..25438
                     /gene="trg"
                     /locus_tag="ACHKA1_002729"
     CDS             23798..25438
                     /gene="trg"
                     /locus_tag="ACHKA1_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO5680357.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRVGGMFMIGAFVFALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHYA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITTVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVLAEPRGAGKPVSFATV"
     gene            complement(25477..26400)
                     /gene="ydcI"
                     /locus_tag="ACHKA1_002730"
     CDS             complement(25477..26400)
                     /gene="ydcI"
                     /locus_tag="ACHKA1_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5680358.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            26617..27960
                     /gene="ydcJ"
                     /locus_tag="ACHKA1_002731"
     CDS             26617..27960
                     /gene="ydcJ"
                     /locus_tag="ACHKA1_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5680359.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            28185..29840
                     /gene="opgD"
                     /locus_tag="ACHKA1_002732"
                     /gene_synonym="mdoD"
     CDS             28185..29840
                     /gene="opgD"
                     /locus_tag="ACHKA1_002732"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO5680360.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            29980..30204
                     /gene="ydcH"
                     /locus_tag="ACHKA1_002733"
     CDS             29980..30204
                     /gene="ydcH"
                     /locus_tag="ACHKA1_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO5680361.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQHESVKEV"
     gene            30267..30806
                     /gene="rimL"
                     /locus_tag="ACHKA1_002734"
     CDS             30267..30806
                     /gene="rimL"
                     /locus_tag="ACHKA1_002734"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO5680362.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(30798..31778)
                     /gene="ydcK"
                     /locus_tag="ACHKA1_002735"
     CDS             complement(30798..31778)
                     /gene="ydcK"
                     /locus_tag="ACHKA1_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO5680363.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGKSR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            31902..32894
                     /gene="tehA"
                     /locus_tag="ACHKA1_002736"
     CDS             31902..32894
                     /gene="tehA"
                     /locus_tag="ACHKA1_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO5680364.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFIARLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            32891..33484
                     /gene="tehB"
                     /locus_tag="ACHKA1_002737"
     CDS             32891..33484
                     /gene="tehB"
                     /locus_tag="ACHKA1_002737"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO5680365.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFAGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         8540 a         7773 c         8852 g         8582 t
ORIGIN      
        1 atacgccggt tccaccagat gacggcggtg atacgccggt tccaccagat gatggcggtg
       61 atacgccagt tccagacgac ggcggtgata cgcctgttcc gccagacgat ggtggcgata
      121 ccccggttaa acacgatcct gtcgtctata aaaatgacgt aacctgggat caggacgcga
      181 aaaccgtcaa aattcgcgaa accaccttta cctacagcca aaatgccgat gggagttaca
      241 ccttaaccgc gcctgatggc ctgacgacga tagtcaaggg ctggacagtc catgacgaca
      301 gcaacaccgt catctttgat ggcgtaaata cggcaggtgg catcacctgg agctacgacg
      361 atgatgggct catccatatc actaaagagg ccggtgtcgt tgttgatggc acaaccggaa
      421 ctgcgattga ttttggtaat gcgatcatca ccgatcaggg cggcaatacg gcgctgaatg
      481 gcggtactgt catgacggtt gagggtgaca acatcaccct caacaacgac ggtaaaacga
      541 ccgccatcgg cgagggttcg gtcgttggta tccttactgg tgacgatatc actatcaata
      601 acaacggcga gacggaagtt gacggcggta ccgcagtaat catcaatggc gataatgcta
      661 cgctgaacac tgcgggcgac tcgaccatca ccaatggtgg tacgggtagc cttatcaatg
      721 gcgataatgc gcgggtcgat aatcaaggta ccatgagcgt tgacggtgag aattccactg
      781 gctcgaaaat tgtcggtgac ggtgccacca tcaaacagga aggcgactta tacgtaagcg
      841 gtggtgcgca cggtattgac gttgacggca atgatacgtt cgttagcaac aaaggtaata
      901 ttacggtcat cgaagataac tctattggca tgctgcttga tggtgatggc gtcagcgtga
      961 tcaacatggg cgacctcaat gttggtcagg ccgctgcggg tgaaaatgca atcggcattc
     1021 agatcgacgg tgataatgcg acatttgtta acgttggtga tatttccgcg accaatgccg
     1081 gaaccggtgt cagcgttgct ggggataaag ctaatatttc actcgcgggt gggttagacg
     1141 ttggggattt ctccaccggt cttgatgtct ctggcaataa taacaacatg acgctggcga
     1201 cttatgagct gaatgtgaca gggcaaaaag cgacgggggt taatgtttcg ggtgatggca
     1261 ataggattga aatcgcaggc agcattctgg ttgataaaga tcagaaagcc gataatgcgc
     1321 aaccttactt ctttaatccc tcaaccggcg taaatatcag cggtaataac aatgacgtca
     1381 cgctggatgg tcagttaacc gttgttgctg atagcgaaac cacggtgcgt tcctatgcaa
     1441 gttacgacgg cgctcaggag cacattgcag gtattgttat tgctggcgat gataatacct
     1501 ttacgcttaa tggcggtgtc cattttgtcg gcgaaaaaaa tgtcatggat gatggctcaa
     1561 aaccatctgc ttctcgtcgt ggtatcggag acacaccgct tattagcgtt gatgggcatt
     1621 catcggtcta cctcaatggt gagtcaacca ttagcggcga gttcccgttg ggtttcgaaa
     1681 atctcattca gttgagtcat ggcgcggagc tggagattgg cgctgacgcg acgtttgata
     1741 tgagtgatgt ggattccttc acctattatt atcgcgtggc gttatccacc attagtattg
     1801 attctggtgc gaaggcgacc aataacggtg aagttgaact gaaaaatatc ggttttgcgg
     1861 ctgcctggaa taaagattca acggtcatca ataacggatc cattggcctg gcgatgtacg
     1921 attttggtac cgacccggca ccaaaagtct tcgatgttga atacgggggg attggcgtta
     1981 acaatggcac cttgaccacc aaaatgatga atcagcacag cgtgctgaat tatccggcag
     2041 agtggaactt gtctgacggt acctctttca ataacagagc attaggtttg acaggtatgc
     2101 ttgccagcta ctcgtcatcc attctgaacg gcgaaacggg cattattgag atgtatggtc
     2161 gtggcagcgt ggggatgttg gctatcgata aatcaaccgc ggataacgaa gggcaaatca
     2221 ccctggatac gctgtgggtc gatgaaaatg atgaaacatc gctgcgtaat aacatcgcaa
     2281 atagcacggc gaaagatttc ggtgtgggaa tggcttcggg aaccgatgct tactatggag
     2341 cattaaccaa agcgacgtct accaaccacg agaatggtgt gattaccgtc tacaacgcgg
     2401 gtgcggggat ggcggcgtat ggtaatacca acacggtcat taaccaggga acgatcaacc
     2461 ttgagaaaaa tgaaaattac aacgactcgc ttggcgtaaa taaactcgtc ggcatggctg
     2521 tttacgagaa aggaacggct atcaatgatc aaaccggtgt tattaatatt aatgctgaaa
     2581 atggtcaggc attctataac gatggcactg ggcttattat caactatggc accatttgta
     2641 cctttggcgt ttgtcagggg gccgatgagt ataatccgac ggataactat atcgccgatt
     2701 tgtatgatga tggcgatgta ttaagctctg cgggtgagac tgtatcatta tcatctggtg
     2761 cgattattaa tggggatgtt accagcgcgg gtaccgttaa gaatggaaaa atccagattc
     2821 ttgaaaatgg cgcgctggtt aatgagtcga gcggaaccat caattcggct atcgtcgtta
     2881 atgcaggcgg tacgttaacc aatgaaggtg tattgaatac ggtgacgctg gaaggaggca
     2941 ctttcaataa caacggaacg cttaatgacg tcgtaaaaat tgagaaaaac agcaacgcgg
     3001 taattaataa taccggttca ctgtcaacct tacagcttaa cgatggcacg gtgaataaca
     3061 gcggtatcgc gtcggcgcgc gttaacgccc agggcgatgc tgtattcaat aaccttgcag
     3121 gcggcgaggc gcgtaaaggt gccattctct ataactctgc ggtggtgaat aacgcgggga
     3181 cctggaaaat gggttatcag gatgaaaata acaatgccgg gacgctggat attgacgacc
     3241 aatcaacgtt caacaacagc ggcaagctca tccttgataa cagtaaaaac gccattcgct
     3301 tccagggaag caatgctaac gccacgttat ataacaccgg tgaaatgacg ctggatgccg
     3361 cacttggcgc gggcgctatt cactatgacg acggcgccag ccagtttatt aacaagggcg
     3421 tggtggatgc gaaagtcact gtggcggtaa gtaccgctgg tgcgacagaa agcgatgcca
     3481 tcctgtggaa ccaggatggc ggggtgatta acttcgataa agacaacgcc agcgcagtta
     3541 aattcaccca caacaactat gttgccctca acgatggcgt gatgaacatc agcggcaaca
     3601 atgccgtggc gatggaaggt gataaaaacg cacaactggt taacaacggc gttatcaata
     3661 tcggcaccga aggtaccacc gataccgggt taattggtat gcaactggat gccaacgcta
     3721 ccgcagacgc ggtgatcgag aacaacggca cgattaatat ctatgctaac gattcctttg
     3781 cattcagtgt gttgggaaca caaggccata tcgttaacaa cggtaccgtg gtgattgccg
     3841 atggcgtgac cggttccggg ttgattaagc agggcgacag cgtgaacgtg gaaggggtga
     3901 acggcaacaa cggtaataat accgaagtgc attacaccga ctacacgttg ccggaagtgc
     3961 cgaacaccag ttctggtggc gtaacaacag ggggagatgc gactggcagc ggtagcaaca
     4021 acctcaacgg ttatgtggtc ggtaccaacg ttgacggcag cgccggtaag ctcaaggtca
     4081 acaacgccag catgaacggt gtcgggatca acaccggttt cgccgccggt acggcagaca
     4141 ccacggtcag tttcgacaac gtggtggaag gcagcaacct gaccgacgcc gatgccatca
     4201 cctcaacgtc cgtggtgtgg accgccaaag gcagcaccga tgccagcggc aacgttgacg
     4261 tcaccatgag caaaaacgcc tacaccgatg tggcgaccga tgcttcggtg aacgatgtgg
     4321 cgaaggcact ggatgcgggt tacaccaaca acgagctgta taccagcctg aacgtcggca
     4381 ctactgccga actgaacagc gccctgaagc aggtgagcgg cagccaggca accacggtat
     4441 tccgcgaggc gcgcgtgctg agcaaccgct tcagcatgct ggcggacgcc gcgccgaaga
     4501 tgggcaacgg tctggcgttt aacgtggtgg cgaaaggcga cccgcgtgct gaactcggaa
     4561 ataacaccga gtacgacatg ctggcgttgc gtaaaaccgt tgacctgagc gaaagccaga
     4621 gcctgagtct ggagtacggt atcgcccgtc tggatggcga cggtgcgcag aaagcgggcg
     4681 acaacggcgt aaccggtggt tacagccagt tcttcggcct gaagcaccag atgttcttcg
     4741 acaacgggat gaactggaac aacgccctgc gctatgacgt gcataacctc gacagcagcc
     4801 gctcggtggc ttacggcgac gtcagcaaaa cggcagacac ggatgtgaaa cagcagtacc
     4861 tggagttccg cagcgaaggg gcaaaaacct ttgaaccgcg cgaagggctg aaaatcaccc
     4921 cgtacgccgg agtgaaactg cgtcacacgc tggaaggcgg ctatcaggag cgcaatgccg
     4981 gagactttaa cctgagcatg aacagcggca gcgaaacggc ggtggacagc atcgtcgggc
     5041 tgaaactgga ctacgcaggg aaaggcggct ggagcgcgaa tgcgacgctg gaaggcgggc
     5101 cgaacctgag ctacagcaag agccagcgca cggcaagcct tgcaggggca ggcagccagc
     5161 actttaacgt cgatgacggt cagaagggcg gcggtatcaa cagcctggcg agcgtcggcg
     5221 tgaagtacag tagcaaagaa agttcgctga atctggatgc gtatcactgg aaagaggacg
     5281 gcatcagcga caaaggcgtg atgctgaact ttaagaaaac gttctaattt ttagcatgtg
     5341 atacacaaac cgcaacgctg atacaggttg cggtttttta ttgccggatg cgacgcttga
     5401 cttatctttt gctgtctaat gttttggtgt ctttcaggac aatagtgcta ctcatccaga
     5461 aatcttgttt acgccttcgc attatttatc tcttttcgtt tctatactga tttttcttaa
     5521 tccgttttat tacagggcag ggtgcgatga gcagcaatac atttactctc ggtacaaaat
     5581 ccgttaaccg tcttggttat ggcgcgatgc aactggcagg tcctggggtt tttggccccc
     5641 caagagatcg ccacgtcgct ataaccgtgc tgcgtgaggc gctggcattg ggcgtcaatc
     5701 acattgatac cagcgacttt tatggtccgc acgtcaccaa tcagattatc cgcgaagcgc
     5761 tttatcctta ctctgacgat ctgacaattg tcactaaaat tggtgcgcgg cgtggagagg
     5821 acgcatcctg gttgcccgca ttttctccgg cagagctgca aaaagcggta cacgataatc
     5881 tacgtaatct cggcctggac atactggatg tggttaacct gcgcgtgatg atgggggatg
     5941 gtcatggccc ggcggaagga tcgattgaag ccagcctgac cgtgctggca gagatgcaac
     6001 aacaaggcct ggtaaaacat attggcctga gcaacgtcac accgacgcag gttgcagagg
     6061 cgcgcaagat tgccgaaatc gtctgtgtgc agaacgaata caacattgct catcgtgctg
     6121 atgacgcaat gattgaagct ttggctcgtg acggcattgc ctacgtgccg ttctttccgc
     6181 tcgggggctt tacgccgctg caatcttcca cgctttccga tgttgctgcg agcctgggtg
     6241 caacaccaat gcaggtggcg ctggcgtggc tgttacaacg ttcaccgaat attttgctga
     6301 tcccagggac gtcttctgtt gcgcatttac gggagaatat ggctgctgaa aaattgcatc
     6361 tttctgagga agtgttgtct acgttggatg gtatttcgcg agaataacga atatacaaaa
     6421 gggaaagatg catttccctt ttgtttcttt tttaatggca tggagtgcat atgtttaagc
     6481 ccaggatttg cagctggata ggtttactcc ccctgtttat gctgagtctt ccagtacagg
     6541 cggaacttcg atgtgttgca aatgcagttg atattgaacc atttttttct gcggctaccg
     6601 ctgaagataa acaacaagtt gaacaagcta tcaacagtag cgtgaatctt gtccccttcg
     6661 gtttatctgc atcggactgg aaagtgcatc gtggcgattt agtggtagaa ggtaatatag
     6721 agagtaatca aaaattgatt gttcttggca atttgacagt caaaggtaat atttccactt
     6781 tctctctttc taatccatgg gttattctcg gtaacgtgac ggcaacgaat atagttacgg
     6841 attcaccact gttaattacc ggttcgataa atgcaagtgg actggtattt atcgactcat
     6901 attacgataa tccgtctacg attaagggga gtattaatgc gcgtgggata tttatcaatg
     6961 acataattgc acctgttgtt gcgtcttcga caaatagtga attcatggtc cgtgcgagtg
     7021 ataaaaatga cactgaaaat gtcaaaaaag cgttgaagat aataaatcct gatgcatatt
     7081 attggggtct aataaatgat gaagatgctc tgaaagaaat ttttaagcga agcaatattc
     7141 gcatggcagg gaatgtctgt aatcagatga aaaaagaagc gctgtttcgc cctaagcctt
     7201 ctcctgagtt agtgcaggaa ttgcaaatgc tggatgaagg caacgttgct gcatttgaag
     7261 gacgagatat tgcgacattt gatcttgcca taatgcgaac tctcccaagg cttaaaggaa
     7321 tttcagctaa cctacgcaaa caacttatta atagcaatga tggacaaacg attgcaagta
     7381 tggcgagata tatgcctgac aatgagattc tggagttgac cgatcagcaa ttgggttacc
     7441 agcctgttgt tctggggttg ctcgaccgtg aaccgctctc tgtcgaaata atgacgcgaa
     7501 tgagtcatct acctgatggt gtcggtccgt tgaatcttgc gctacgtgaa aatctccctc
     7561 tggatatcgt catgactctg gcgaaaagag attgggatat gatcattcag gagctttata
     7621 aagatgcgtg gttattgccc gaatccatta ttgatggcta tatccgcagt gatgattcct
     7681 ctattcgtca ggtcggtgct ggaggacaac tcacctacaa tcaggcaatg cagttggcga
     7741 acgattcatc aaacgatgtt gtcacaagct tagcgttaaa gctggcagag atgaaacacc
     7801 atggtcaatt gttgcggatg acgccacaag agagtgacaa aatagcagta tatttatacc
     7861 aaaaattcga gaatgacgat gatctaatag gtgcgttatt tttagcgttg ccagataact
     7921 tacagtttaa ttttgttaaa aggatggaga aaaaatcccc tgcctacttt tgctgtcggg
     7981 atatgcagat aattcactct gacgctgctt tacaacggtt attgacacgt ttcaacgatc
     8041 ctgaggggtg gagtaatctg gcgaaaaatc agtatctgag cacgtcgatg aaacaaaaaa
     8101 tttggcaacg tgctttatcg catcggaaaa ataatcccaa agcagattca gatgcatatg
     8161 aaaccagcgc tgatatgatt ttgtctgagc ttatcagcta cggtgaagtt gatgaccaga
     8221 tgctattaaa tgccacctct ctgatacgtt cggacgactg gggcttttta gagagtgcat
     8281 taattagttg ggataattta cccgctgttg ttctcaagga attacagcaa aacacatcac
     8341 gcaatgatat ttgggcgaag ttttttctga ggcaggaaaa cagttcccgt gcgcaggtta
     8401 acgaagcgtt acgtgtttat tatgcactag accccgatgc gttagcacaa ctggatgtac
     8461 tggcaaaaca accggatcgt atatggtgga gtacactggc gaaaagcaat ctcacatttt
     8521 tcaagttcgg cgcacttaac aaccgccaca caccgcctgc agtactggcg gcagaaattg
     8581 atcccgagtg gtggattgtg gcgatgaata atccccgttt tccagttgat gtattaaagg
     8641 cgaggctgaa acgtgatcct ttgctggcgt tagagcttgt taaccctgaa ctggatttag
     8701 ttcgccagct ggcgcttaac ggtaagacgc gcgcaatacg ggaacaagcg atgagaaaac
     8761 ttgatgagtt gtattgaata acagcgtaat aatttttaac cacttgattt attggggata
     8821 atcattcctg acagtgagtc cccaatacct tgatatattc tgaattttta atgaagcgtc
     8881 gtgttgcgat atctccgtca ggggaattga tgcaccatag cgcaaaccga attatcaagg
     8941 attgataatg acgctctacc agataaaacc gctctttcag tcgctgttaa ggccgacgat
     9001 gttttggctt tataagcacc atgttacagc gaatcacatc actcttgctg cactggcgct
     9061 ttctcttatt accggattgc tgttgatgtt ggtcgcacaa cccatcctct ttttgctatt
     9121 gcccatcgtg ctttttatcc gtatggcact caatgcgctg gatggcatgt tggcgcgtga
     9181 gtgcaatcag caaacacgtc tgggggcgat tttgaatgaa actggcgatg ttatttccga
     9241 tattgcgctc tatttaccgt ttttattttt accagaaagt aacgcatcac tcgtaatact
     9301 catgttattt tgcaccatat tgactgagtt ttgcggttta ctcgcgcaga cgattaacgg
     9361 tgttcgcagt tatgtcggtc cttttggcaa aagcgatcgc gcgttaatat ttgggttgtg
     9421 ggggctggcc gttgccattt atccacagtg gatgcaatgg aataatcttt tatggagcat
     9481 tgcttcaatt ctgcttctct ggactgcgat taatcgttgt cggagtgtgc tcctgatgag
     9541 tgctgaaaga taatgctgga aaaatctctg gcaacacttt tcgcgttgtt aattttagca
     9601 acgctgataa accgttttcg ggtatggcgg ttaccggaga gaaaagggga tgaagttaca
     9661 ttacgtattc gtacctggtg gggcatcgtc atttgttttt cactggtgat ttcaggtcca
     9721 cgctggatga cgttgacgtt ttttgcgctg ataagttttc tggcattgaa agaatattgt
     9781 acgcttatat ccgtacattt tccgcgttgg ttatattggg ttattcctct taactatttg
     9841 cttatcggtt ttaattgctt tgagctgttt ctgttattta tacctttggc tggggttctg
     9901 atattaacca cctggcgagt tttagtaggt gatacctccg gttttctgca taccgtgagc
     9961 gccatttttt ggggctggat aatgaccgtt ttcgccttga gtcatgccgc ctggttatta
    10021 atgttgccaa ccacaaatac ccagggcggg gcgttactgg tgttatttct tttggcgtta
    10081 acagagtcaa acgatattgc acagtattta tgggggaaat cctgcggcag aagaaaagtg
    10141 gtccccaaag tcagcccggg aaaaacgctg gaaggtctgg tggggggcgt aatcaccacc
    10201 atggttgcgt cactgattat tgggccgttg ctgacaccgc taaatacatt acaggcatta
    10261 ttagcgggtt tgttaattgg tattagtggt ttttgcggcg atgtagtgat gtcagccatc
    10321 aaacgagata ttggtgttaa agatagcgga aaactattac caggacatgg cggactcctt
    10381 gacaggattg actcattaat tttcaccgct ccggtatttt tttattttat tcgctactgc
    10441 tgttactgaa ggaaaataga gaaaatggaa aattcacgca tccctgggga acattttttt
    10501 accaccagtg ataatacagc gttgttttat cggcactggc ccgctttaca gcccggggcg
    10561 aaaaaggtca tcgtcttatt tcatcgcggt catgaacatt ctggtcgtct acaacatctc
    10621 gttgatgaac tggcgatgcc agatactgct ttttatgcat gggatgcccg agggcatgga
    10681 aaaacttcgg ggccgcgtgg ttatagccca tctcttgcgc gttcagtgcg ggatgtcgat
    10741 gaatttgtcc gttttgctgc cagcgacagc caggttggac tggaagaggt ggtagtgatc
    10801 gcgcaaagcg tcggcgcagt gctgattgcc acatgggttc atgattatgc gcctgcaatt
    10861 cgcgggctgg tgctggcttc tccggccttt aaggttaaat tgtatgtgcc gctggcacgt
    10921 cctgcgctgg cgttatggca tcgtctgcgt ggtctgtttt ttattaattc ctatgtgaaa
    10981 ggacgctatt tgacccacga tcggcaacgg gtggcgagtt tcaataatga tccgctgatc
    11041 acaagggcga ttgccgtcaa tatcttgcta gatctctata aaacgtctga acgtattgtt
    11101 agcgatgcgg cggcgattac gctccccacg caacttctga tatcaggcga tgactatgtg
    11161 gtgcatcgcc aaccgcagat tgatttttat cagagattac gtagccctct gaaagagctg
    11221 catctgctgc caggctttta tcacgacacg ttgggtgaag agaacagggc gcaggcattt
    11281 gaaaaaatgc aaagctttat tagtcgttta tatgctaaca agtcacaaaa atttgattat
    11341 cagcatgaag accgcactgg accatcagcg gatcgctggc ggctcctttc aggtggaccc
    11401 gtgccattat cgccggttga tttggcgtat cgctttatgc gtaaagcgat gaaattgttc
    11461 gggacgcact ctgcgggcct gcatctcgga atgagcaccg gctttgattc aggcagttcg
    11521 ctggattatg tctatcaaaa tcaaccgcaa ggtagtaacg cattcgggcg cttaatcgac
    11581 aaaatctacc tgaacagtgt tggctggcgc ggtattcgcc agcgcaaaac ccatttacaa
    11641 atactgatta aacaagccgt tgccgatctc cacgccaaag gtttagccgt ccgcgtggtt
    11701 gacattgccg cagggcatgg gcgctatgta ctggatgcgc tggcaaacga acctgccgta
    11761 agcgatattt tgttacgtga ttacagtgag ttaaatgttg ctcaggggca agagatgatt
    11821 gcccaacggg gaatgtctgg gcgggtgcgt tttgaacagg gcgatgcgtt taacccggag
    11881 gaactcagcg cgttaactcc gcggcctacg ctggcgattg tttctggact gtatgagctt
    11941 tttcccggaa atgagcaggt aaaaaactca ctcgcaggtc ttgccaatgc catcgaaccg
    12001 ggtggcattc tcatctacac cgggcagccg tggcacccac aactggagct gattgccggg
    12061 gtgttaacca gtcataaaga tggtaaaccg tgggtaatgc gcgtgcgttc gcaaggggag
    12121 atggattcgc tcgtgcgtaa tgccggattt gataaatgca ctcaacggat tgatgagtgg
    12181 ggcattttta cggtttcgat ggcggtgcgt cgtgataact gaacgccgga acgtattgct
    12241 acaaggcgct ggctggttat tgttgctggc cccgtttttc ttcttcacct atggatctct
    12301 taatcagttc accgcggttc aggactttaa cagccatgat atccccagtc aggtattcgg
    12361 ctgggaaacg gcgatccctt ttctcccctg gactattgtt ccttactgga gtctggatct
    12421 tttatatggg ttttcgttgt tcgtttgtag ttcgacattc gaacagcgcc gactggttca
    12481 ccggcttatt ctggcaacga taatggcctg ctgcggtttt tttctctatc cgctgaagtt
    12541 tagttttatc cgtcctgaag tgagtggggt gacaggatgg ctattttcgc aacttgaact
    12601 gtttgatctg ccttataacc agtctccttc gctgcatatt attctctgct ggctactttg
    12661 gcgtcacttt cgtcagcatc tggctgtgag gtggcgtaaa gtctgcggcg gatggttttt
    12721 actcatcgcc atttcgacgc tgacaacctg gcagcatcat tttattgatg tcatcacagg
    12781 gctggcggta ggtatgttaa ttgactggat ggtgcccgtc gaccgtcgtt ggaattatca
    12841 gaaacctgat caacgtcgaa tcaaaatagc actgccatat gtcgtaggcg cgtgctcgtg
    12901 cattgtgttg atggagctaa tgataatgct tcagttatgg tggtcagtct ggttatgttg
    12961 gccagtatta tcgctattca tcattggccg tgggtacggt gggcttggcg cgataacaac
    13021 agggaaagat agtcagggga aactcccgcc cgccgtttac tggctgacat tgccctggcg
    13081 catcgggatg tggctgtcta tgcgttggtt ttgtcttcgc ctggagccgg tgagcaaaat
    13141 gactgctggt gtttatttag gggcgtttcc acgacatatt ccggcacaga atgcggttct
    13201 ggacgtcacc tttgaattcc ctcgcggacg agccacaaaa gatcgactct atttttgtgt
    13261 accgatgctg gatctggtgg ttccggaaga gggggagctc cgacaggccg tggcgatgct
    13321 ggaaacatta cgcgaagagc aaggcagcgt tctggtccat tgtgcattgg gattatcgcg
    13381 cagtgcgctg gtggtggcgg catggttgtt atgttacgga cactgtaaaa ccgttaatga
    13441 agcgattagc tatattcgag ccagacgccc gcagattgtg ctgacagacg aacacaaagc
    13501 aatgctgaga ttgtgggaaa acaggtaagt ggattgagat gtggactgaa tatctacagt
    13561 ccacatcaag accgtgtccg gttatgcagc aacaatgctg tcgatggctg ctttcgcgtc
    13621 agactgcgct ttcgctgcca tttccggacc gtatgcgatc ccttcggcga agacaaattt
    13681 cacatcagta atgccgataa agcccaggaa cgtggacaga tacggcgtca ccaggtccgt
    13741 tggtccatct ttgtggatcc caccgcggct ggtaataacg atggcttttt tacccgttac
    13801 cagaccttcc ggaccattct cggtatagcg gaaagtaacg cctgcacgtg caaccaggtc
    13861 aaaatagttt ttcagctgag tcgagatgtt gaagttatac atcggtgccg caataacgat
    13921 aacgtcatgg gctttcagct cggcaatcag ctcatcggaa agtgccagag cttcctgctg
    13981 acgcggagtc agcggcgcat cgctcggacg cagcgcacca accagttcgc catccagtac
    14041 cggaatcgga tttgcagcca ggtcgcgaac ggtgatttca tcagcggagt gcttttcgcg
    14101 ccattgttca acaaaataat cggacaactg attagactga gagtaccctg ccaggatgct
    14161 ggatttaaga actaatacct tgctcatggt gtttccttat agatgtttga atgggcgatg
    14221 ccccgttgct tgctgacact ttattcacaa tcctgccaca gagatagcgc aataaatcga
    14281 agcctatgtt cgaatttatt gaacaacgca tagaaagccg cgatgtggta ctctatatct
    14341 atcatttaaa agaaaattaa tcaggcagcc tactgccctc taacgttatg acagaacaac
    14401 aaaaattgac ctttacagcc ttgcagcagc ggctggattc gctgatgctg cgtgacagac
    14461 tgcgtttttc tcgccgtctg catggtgtga agaaggttaa aaatcctgat gcacaacagg
    14521 ccattttcca ggagatggcg aaagagattg accaggcggc agggaaagtc ctgctgcgtg
    14581 aagcggcacg accggaaatt acttacccgg acaatttacc agttagtcag aaaaagcagg
    14641 acattctcga agcgattcgt gatcaccagg tggtgatcgt cgccggggaa accggttccg
    14701 gtaaaacgac tcagctaccg aaaatctgta tggagctggg gcgcggtatt aaagggctga
    14761 tcggtcatac ccagccgcgt cgtctggcgg caagaacagt ggcaaaccgt attgcggaag
    14821 agctgaaaac ggagccgggc ggttgcatcg gttacaaagt gcgtttcagc gatcacgtaa
    14881 gtgataacac gatggtcaag ctgatgaccg acggtatcct gctggcggag atccagcaag
    14941 accgcctgct gatgcagtac gacactatca ttattgacga agcgcacgaa cgcagcctga
    15001 atatcgattt tttgctcggc tatttgaaag agttgctacc gcggcgtcct gacctaaaaa
    15061 tcattatcac ttccgcgact atcgacccgg aacgcttttc gcgccacttt aataatgcgc
    15121 cgattatcga agtctccggt cggacctatc cggtggaagt gcgctatcgc ccgattgttg
    15181 aagaagccga tgacaccgag cgcgatcaat tgcaggcgat ttttgacgcc gtagacgaac
    15241 tgagccagga aagccccggc gacattctga tctttatgag cggcgagcgg gaaattcgcg
    15301 ataccgctga tgcactgaac aaactgaact tacgacatac ggaaatcttg ccgctttatg
    15361 cgcggctttc gaatagcgaa cagaataggg tgttccagtc gcatagcgga cggcgcattg
    15421 tgctggcgac caacgtcgcg gaaacgtcgc tgaccgtacc ggggattaaa tacgttatcg
    15481 accccggtac agcgcgtatc agccgctaca gctatcgcac caaagtgcag cgtttgccga
    15541 ttgagcccat ttcgcaggcg tctgccaatc agcgtaaagg ccgctgtggt cgtgtgtccg
    15601 aagggatctg tattcgtctc tattccgaag acgatttcct ctcgcgcccg gagtttaccg
    15661 atccggagat tctgcgtacc aacctggcct cggttatttt gcagatgacc gcgctggggc
    15721 tgggcgatat cgctgcgttc ccgtttgtcg aagcaccgga taaacgcaat atccaggatg
    15781 gcgtgcgtct gctcgaagaa ctgggcgcga tcaccacgga tgaacaggcc agcgcctata
    15841 aactgacgcc gctcggtcgc cagctctcgc agttgcctgt cgacccacgt ctggcgcgta
    15901 tggtgctgga agcgcaaaaa catggctgcg tgcgtgaggc gatgattatc acgtctgcgc
    15961 tctccattca ggatccgcgc gaacgtccga tggacaaaca gcaggcatcg gacgaaaaac
    16021 atcgtcgctt ccacgacaaa gagtctgact tcctcgcgtt tgtgaatctg tggaattatc
    16081 ttggcgagca gcaaaaggcg ctttcttcca acgccttccg tcgcctgtgt cgtaccgatt
    16141 atctcaacta tctgcgtgtg cgcgaatggc aggatatcta cacccagttg cgccaggtgg
    16201 tgaaagaact cggcattccg gtgaacagcg aaccggcgga gtatcgtgaa attcacattg
    16261 cgttgctgac gggtttgctt tcccatatcg gcatgaaaga tgccgataaa caagaatata
    16321 ccggcgcacg taacgcgcgt ttctccatct tccccggttc tggtttattc aaaaaaccgc
    16381 cgaaatgggt aatggtggcg gaactggtag aaaccagccg cctgtggggg cgcattgctg
    16441 cgcgtatcga cccggaatgg gtggagcctg tcgctcagca tttgattaaa cgtacctaca
    16501 gcgaaccgca ctgggaacgg gcgcagggcg cggtgatggc aacggaaaaa gtcactgttt
    16561 atggtttgcc gattgttgcc gcgcgcaagg tcaactacag ccagatcgat ccggcgttat
    16621 gtcgtgaact ctttattcgc cacgcgctgg tggaaggtga ctggcagacg cgtcacgcat
    16681 tcttccgtga aaacctgaaa ctgcgggccg aagtggaaga gctggaacac aaatcacgtc
    16741 gccgcgatat tctggttgat gacgaaacgt tgtttgagtt ctacgaccag cgcatcagcc
    16801 acgatgtaat ctccgctcgt cacttcgaca gttggtggaa aaaagtcagc cgcgaaacgc
    16861 ctgatttgct caactttgaa aagagcatgt tgatcaaaga aggggcggaa aagatcagca
    16921 agctggatta tccgaacttc tggcatcagg gcaatctcaa gctgcgtttg agctatcagt
    16981 ttgagcccgg cgcggatgct gacggtgtga ccgtacatat tccgctgccg ttacttaacc
    17041 aggttgagga aagcgggttt gaatggcaga tccccggtct gcgccgcgaa ctggtgattg
    17101 ctctgattaa atcgttgccg aaaccggtac gccgtaattt tgtacccgcg ccaaactacg
    17161 ccgaagcgtt tttaggccgc gtcacaccgc tggagttacc gttgcttgac agccttgagc
    17221 gcgagttacg gcggatgacc ggcgttaccg ttgaccgcga agactggcac tgggatcagg
    17281 tgcccgatca cctgaaaatc accttccgcg tggtggatga caaaaacaag aagctaaaag
    17341 aagggcgctc gctacaagat ctgaaagatg cgctgaaagg caaagtgcag gaaacgctgt
    17401 ctgcggtggc ggatgacggt atcgagcaga gcggcttaca tatctggagt tttgggcaac
    17461 tgccggaaag ctacgagcag aagcgcggca actacaaagt gaaggcgtgg ccggcgctgg
    17521 tggatgagcg cgacagtgtg gcgatcaaac tgtttgataa cccgctggag caaaaacagg
    17581 caatgtggaa cggtcttcgc cgtctactgc tgctgaatat tccatcgccg atcaagtatt
    17641 tacatgagaa gttaccaaac aaagccaagc ttggactgta ctttaacccg tatggcaaag
    17701 tgctggagct gatcgacgac tgtatctcct gcggtgtgga taaattgatc gacgccaatg
    17761 gtggcccggt ctggacggaa gaaggctttg ctgcgctgca tgaaaaagtg cgtgccgaac
    17821 tgaacgacac ggtggtggat attgcgaagc aggtcgagca aatccttacg gcagtgttca
    17881 atatcaacaa gcgtctgaaa gggcgggtgg atatgaccat ggcgctgggg ctttctgaca
    17941 ttaaagcgca gatgggcggg ttggtatatc gcggttttgt cactggtaac ggcttcaaac
    18001 ggctgggcga cacgctgcgt tatttgcagg cgattgaaaa acggctggaa aagctggcgg
    18061 ttgatccaca tcgcgatcgt gcacagatgc tgaaagtcga aaacgtccag caggcgtggc
    18121 agcaatggat caacaaactg ccgcctgcac gtcgtgagga tgaagacgtg aaagagatcc
    18181 gttggatgat agaagagctg cgcgttagtt acttcgctca acaacttggt acgccttatc
    18241 cgatttcaga taagcgtatt ttgcaggcga tggagcagat tagcggttaa cgctgctatt
    18301 tgcctgataa atataaaacc cggtaagcat ttagcgccgg gttttttatt aattctaaaa
    18361 cggtaagggc aaaaattcag gaattcagaa aaatacaatt ctctgctgca agatgaataa
    18421 tgtttatcta cagcatttcc ttaaaagata tgtcaggctt gcggagtggc ggttaaggaa
    18481 atacgacatc ctcctttcag agtgctccgc ttctcactat tatctcacgc agtattctta
    18541 agggaacgat aaggaggaac catgaacatt accccgtttc cgacgctttc gacggcaact
    18601 atagatgcca taaatgttat cggacagtgg ctggcgcagg atgatttctc cggtgaggtg
    18661 ccgtatcagg ccgattgcgt catacttgca ggcaatgcgg ttatgccgac tatagatgcg
    18721 gcatgtaaga ttgcccgcga tcagcaaatt cctttactga ttagtggtgg tatcggtcac
    18781 tcgaccattt ttttgtatag cgccatcgca cagcatccgc actacaacac tatccgcacc
    18841 actggcagag cagaagcgac catcctggcg gatatcgctc atcagttctg gcatattccg
    18901 catgaaaaaa tctggattga agaccagtca acaaactgcg gtgaaaacgc acgctttagc
    18961 atcgcgctat tgaatcaggc cgtagaacga gttcatacgg ctatcgttgt acaggacccc
    19021 accatgcagc ggcgcacgat ggcaacgttc cgccgtatga ccggggacaa tccccatgta
    19081 ccacgctggt taagttatcc cggattcgtt cctcagttag gaaataacgc agacagtgta
    19141 atctttatta atcagttaca aggattatgg ccggttgagc gttatctctc actactcact
    19201 ggcgagctgc cgcgtttacg cgatgatagc gatggctacg gtccccgcgg gcgagatttt
    19261 atcgttcacg ttgattttcc ggcagaagtc attcaggcat ggcaaacgct taaacacgat
    19321 gcggtgctca tcgaggcgat ggaaagtcgc tcgttacgtt aaaaattgcc cgtttgtgaa
    19381 ccacttgttt gcaaacgggc ataactcctg ccttttattt ctgcctctta ttccttttac
    19441 acttgttttt atgaagccct tcacagaatt gtcctttcac gatgccgtct ctctgatgat
    19501 tgatgttaat taacaatgta tttaccaaaa acaaacatat aaatcacagg agtcgcccat
    19561 gtcagtaccc gttcaacatc ctatgtatat tgatggacag tttgttacct ggcgtggaga
    19621 cgcatggatt gatgtggtaa accctgctac agaggctgtc atttcccgca ttcccgatgg
    19681 tcaggccgag gatgctcgta aggcaatcga tgcagcagaa cgtgcacaac cagaatggga
    19741 agcgttgcct gccattgaac gcgccagttg gttgcgcaaa atctcctccg ggatccgcga
    19801 atgcgccagt gaaatcagtg cgctgattgt tgaagaaggg ggcaagatcc agcagctggc
    19861 tgaagtcgaa gtggctttta ctgcggacta tatcgattac atggcggagt gggcacggcg
    19921 ttacgagggc gagattattc aaagcgatcg tccaggagaa aatattcttc tgtttaaacg
    19981 tgcacttggc gtgactactg gcattctgcc gtggaacttc ccgttcttcc tcattgcccg
    20041 caaaatggct cccgctcttt tgaccggtaa taccatcgtc attaaaccca gtgaatttac
    20101 gccaaacaat gcgattgcat tcgccaaaat cgtcgatgaa ataggccttc cgcgcggcgt
    20161 gtttaacctt gttctggggc gtggtgaaac cgttgggcaa gaactggcgg gtaacccaaa
    20221 ggtcgcaatg gtcagtatga caggcagcgt ctctgcaggt gagaagatca tggcgactgc
    20281 ggcgaaaaac atcaccaaag tgtgcctgga actggggggt aaagcaccag ctatcgtaat
    20341 ggacgatgcc gatcttgaac tggcagtcaa agccatcgtt gattcacgcg tcattaatag
    20401 tgggcaagtg tgtaactgtg cagaacgtgt ttatgtacag aaaggcattt atgatcagtt
    20461 cgtcaatcgg ctgggtgaag cgatgcaggc ggttcaattt ggtaaccccg ctgaacgcaa
    20521 cgacattgcg atggggccgt tgattaacgc cgcggcgctg gaaagggtcg agcaaaaagt
    20581 ggcgcgcgca gtagaagaag gggcgagagt ggcgttgggt ggcaaagcgg tagaggggaa
    20641 aggatattat tatccgccga cattgctgct ggatgttcgc caggaaatgt cgattatgca
    20701 tgaggaaacc tttggcccgg tgctgccagt tgtcgcattc gacacgctgg aagatgctat
    20761 ctcaatggct aatgacagtg attacggcct gacctcatca atctataccc aaaatctgaa
    20821 cgtcgcgatg aaagccatta aagggctgaa gtttggtgaa acttacatca accgtgaaaa
    20881 cttcgaagct atgcaaggct tccacgccgg atggcgtaaa tccggtattg gcggcgcaga
    20941 tggtaaacat ggcctgcatg aatatctgca gacccaggtg gtttatttac agtcttaatg
    21001 agtgaaagag gcggaggttt tttcctccgc ctgtgcgcgt cagagtttag cgaatttttc
    21061 gagggtgcga ataagctgcg tgacgaagcc atattcgtta tcgtaccagg cgaccgtttt
    21121 caccagttgt aaatcgccca cggcggtaat ttccgtttgc gtggcatcaa acaccgaacc
    21181 gaaatggctg ccaatgatat cggaagagac tatttcttca tcggtataac caaatgactc
    21241 gttattggtg gttgcttgtt taagtgcgtt attcacctct tcggcagtca ctttttttcc
    21301 gagaatcgat accagttcag tgaccgaacc tgttttcacc ggcacgcgtt gcgcatgacc
    21361 tttcagtttg ccgctcagtt ccgggatcac cagaccaatg gcttttgccg cgcccgtagt
    21421 gtggggaatg atattttctg ccgctgcgcg tgaagcacgt aaatctttac cacgcgggcc
    21481 atccaccagt gactgggtgc cagtataggc atgaatggtc gtcatcgtgc cgacttctat
    21541 cccgaaactg tcatgcaagg ctttggccat cggcgcaagg cagttagtgg tgcatgacgc
    21601 cacggaaaca atggtatcgt tgccatccag agtgtcgtca ttaacgttat aaacgatggt
    21661 tttcatttca ccggcaggag cggaaatcaa caccttcttc gcaccagcat caagatgcgc
    21721 ctgcgatttc tcggcggagg tataaaagcc agtacattcg acaatgattt ctgcaccttt
    21781 cgctttccac ggaatatttt tagcctcttt ttcggcgtaa accgcgatac ttttcccatt
    21841 aacgataagt gaatcttccg taaaatcaac gctccagggg aacggtccgt agtttgaatc
    21901 atgtttcagc aggtaggcga gaatttttgg agaagtgaga tcattaatag cgacaacgtc
    21961 tatgttgctt ttgacttcaa gtaatcgacg caacaccagt cgaccgatac gaccaaaacc
    22021 gttaatacca actttactca tggttttctc ctgtcaggaa cgttcggatg aaaattgatc
    22081 ctttccaggc ttagaccagg atggcgggat gggcaatctc cattcttaca gtgaaacgta
    22141 acgtactgaa aacgggtgaa caatatttaa tgaaattttg agaaaagccc gttatgttaa
    22201 cggaaaatta tgttaaagca ggaaatgtta tggaaaataa atactcaagg ttacaaatca
    22261 gcattcactg gctggtcttt ttgctggtta tcgcagcgta ttgcgcaatg gagtttcggg
    22321 gtttcttccc acgtagcgat cggccactca tcaacatgat tcatgtttcc tgcggcatat
    22381 caattctcgt gctgatggtc gttcgtcttt tgttaaggct gaaataccca accccgccga
    22441 ttatacctaa gccaaaaccg atgatgacgg gactggcgca tttgggacat ttggtgattt
    22501 atctcctgtt tattgcgctg ccagtgattg gtttggtgat gatgtataac cgaggcaacc
    22561 cgtggtttgc gtttggtttg acgatgcctt acgcttcaga ggccaatttc gaacgggtag
    22621 atagcttaaa gtcgtggcat gaaacgctgg cgaatctggg atattttgtc atcggtttgc
    22681 acgctgcggc tgcactggca caccactatt tctggaagga caacacactt ctacgcatga
    22741 tgccgcgtaa acgttcctga aggatattta aagaaaacgc ctgtactaaa accgacccgt
    22801 ggtacaggcg aagaatacgg gtctacatcg gaagcgccta ttatatttat ttgtatgaca
    22861 aataaaacca taatccttgc ccatacgtcc atctggctta tttttaatca attcacccga
    22921 tctttgatct catcaacggt atcaaaataa accagcgtat aacgttcaaa tcgaacataa
    22981 gattcgataa accatggagg ttatatgaaa aaactggcac ttattttgtt tatgggaacg
    23041 cttgtttcct tttatgccga tgccgggcgc aaaccctgtt ctggttcgaa aggggggatc
    23101 tcacactgta cggcaggcgg caaatttgtc tgtaatgatg gttctattag tgcatcgaaa
    23161 aaaacatgca ctaactgaag tgtaaaaggg gtgccatgag aaaatgaatc tgctagtcag
    23221 atgcgcgggg aaaatccccg cgcttgccct tacctggacg tgcaggccat gagcgcagca
    23281 acctccttat caccgtcccg gaaccgcagt tcgtagagcg tttgtcgggt caggagtgtg
    23341 aatgtcagaa tcgtaatgca gataatgagc agacacacca ccagagggtt gtgcttcata
    23401 gcctttctcc ttgccggatg gcgggtaaga ggctaagatc tgaattgcta ggttcattcg
    23461 ttggcctcgg ttgatagaaa tatcggtcgg ggccttcgtc tttctgattc ccggttagcc
    23521 tgaaaacaga aagtctcagg cacccgtgag catcctatgt ggtttcctta gggacgaaaa
    23581 taatcacctg acgaaatttc gtgctgtttt ccagaatttt tcgtcattcg ggttagccag
    23641 tttaaccatt cgttactctc ttcattccaa tggcattaat cttctctgca ataattgttg
    23701 taaaaatgtg acgcaaagag gcttttagtc gtaagtaatt accgtcaaat gccgatgact
    23761 ttctatcagg agtaaacctg gacgagagac aacggtaatg aatacaactc cctcacagcg
    23821 attaggtttt ttgcatcaca tcaggttggt tccgttattt gcctgcattc taggcggtat
    23881 cttagttcta ttcgcattaa gttcagccct ggctggctat ttcctctggc aggccgatcg
    23941 cgatcagcgt gatgttactg cggagattga gattcggacc gggttagcga acagttcaga
    24001 ttttttgcgt tcagcccgga tcaatatgat tcaggccggg gctgcgagtc gtattgcgga
    24061 aatggaagca atgaagcgaa atattgcgca agccgagtcg gagattaaac agtcgcagca
    24121 aggttatcgt gcttatcaga atcggccggt gaaaacacct gctgatgaag ccctcgacac
    24181 tgaattaaat caacgctttc aggcttatat cacgggtatg caacctatgt tgaaatatgc
    24241 caaaaatggt atgtttgaag caattatcaa tcatgaaagt gagcagatcc gaacgctgga
    24301 taatgcttat accgatattt tgaacaaagc cgttaagata cgtagcacca gagccaacca
    24361 actggcggaa ctggcccatc agcgcacccg cgtgggtggg atgtttatga ttggcgcgtt
    24421 tgtgtttgcc ctggtcatga cgctgataac atttatggtg ctacgtcgga tcgtcattcg
    24481 tccactgcaa catgccgcac aacggattga aaaaatcgcc agtggcgatc tgacgatgaa
    24541 tgatgaaccg gcgggtcgta atgaaatcgg tcgcttaagt cgtcatttac agcaaatgca
    24601 gcattcactg gggatgacag tagggactgt tcgacagggc gcggaagaga tttatcgtgg
    24661 caccagcgaa atttccgctg gcaatgcgga cctttcatct cgcaccgaag aacaagcggc
    24721 ggcaattgaa caaactgccg ccagcatgga gcaactcact gcgacggtga aacagaatgc
    24781 ggataacgcg cattatgcca gtaaactggc gcaagaggct tctattaaag ccagcgatgg
    24841 cgggcagacg gtttccggtg tagtaaaaac gatgggcgct atctccacga gttcgaagaa
    24901 aatttctgag atcaccaccg tcatcaacag tattgctttc cagacgaata ttctggcact
    24961 gaatgctgcc gttgaagctg cgcgagcggg tgagcaaggg cgtggatttg ccgttgtcgc
    25021 cagcgaagta cggacactcg caagccgcag cgcccaggcg gcgaaagaga ttgaaggctt
    25081 gatcagtgaa tcagtcaggt taattgacct gggatcggat gaggtggcaa cggccgggaa
    25141 aaccatgagc actattgttg atgccgtcgc gagtgtcaca catatcatgc aggaaatcgc
    25201 cgccgcctcg gatgagcaaa gtagaggcat aactcaggtt agccaggcga tttctgaaat
    25261 ggataaggtg acgcaacaga atgcttctct ggtagaagag gcctcagcgg cggcggtgtc
    25321 ccttgaagaa caggcggcac gattaactga ggcggtggac gtattccgtc tgaacaaaca
    25381 ttctgtgttg gcagaacctc gcggagcggg taaaccagtt agtttcgcta cggtgtgaaa
    25441 atgttcaagg aagggatcga cagatccctt cacctttcag aacggcattg attttcgaat
    25501 agcgttaatc atcaactggc aaccagaaga gaacgtcgca tctacgcggg tcagtattcc
    25561 aatcggttcg cctgcaccat gtcccggaac aggcagggcc accagcgtgg catgacgcag
    25621 gtcgtctttt acagcgccag aagggacaaa ccacacgtaa tcgtattcaa ccgtaagttg
    25681 acgggatagc gaagcagaca gcgtttcgat acaacccgaa ggaattttac atccctggct
    25741 ctgtactaat gcatctgaat gctggcgtgg tgcagtgcct tctggtgata caacgaccgg
    25801 ccattccagc actcggctta gcgttacgtt ctcctgaagt agcgggtgat tagggcggac
    25861 aaccagcttc agcgattcaa gaaacagcag ttcgtaatta agcccggtca tcagttcagg
    25921 atctgacatc cgaccaatgc cgatatcgat ttccccggtt tttaaacccg ccagaatcat
    25981 agggttactc attgtcgcaa cttgcaaggt cgtctctttt tgttgctgat gaaactgacc
    26041 tataaccgaa ggtaatatcc ccagtgccgc agtaggtagt gcaccaaccc tgacgacatc
    26101 attattaaga ccttctttac gatgaagcga ctgtccagca gtgttgatgg cgtcaagaac
    26161 tctgactgca tgcgttaaaa attgttcgcc gggtaaggta agttgcgccc cctgacgacc
    26221 acgctcaaac aagcgagcgc ccgtcagttg ctccagttca ttcaatgtct tagagagcgc
    26281 aggttgactc aaattaaggg tttcagccgc gcgccccaaa gttccttgtt gtgcgacagc
    26341 tacgaatgta tgaaggtggc gcaaacggat gcgctgacta aacagactat ttttttccat
    26401 aagcgatgtt aaaaacgaag cggtgtcgct gacaagtgaa gttgtttgat tatgataact
    26461 tgattgcaaa atattattaa caattaaagc aattatgtta cagcaaaatg gataatattg
    26521 atgttttcgc ggcgagatca cagtttgtaa attcttcccg ccagagtgaa tgcggttacc
    26581 tacactccag attactgacc actggaggca gacactatgg cgaacagcat cacggcggat
    26641 gagattcggg aacagttttc gcaggcaatg tcagccatgt accagcaaga agttccgcaa
    26701 tatggcacgc tgctggaact ggtagctgat gtgaatctgg ctgtgctgga aaacaatcct
    26761 caactgcacg aaaaaatggt aaatgcagac gagctggcgc gactgaatgt tgaacgtcat
    26821 ggggcgattc gcgttgggac tgcacaagag cttgctactc ttcggcggat gtttgccatt
    26881 atggggatgt acccggtgag ctattacgat ctctcgcagg caggggtgcc ggtacattcg
    26941 acagcatttc ggcccattga tgatgcttct ctggcgcgta atcccttccg cgtttttacc
    27001 tccttactcc gccttgagct tatcgagaac gaaattttgc gccagaaagc ggcggagatt
    27061 ctgcgtcagc gcgatatctt caccccacgt tgtcgacaac tgttagagga atatgagcag
    27121 cagggcggtt ttaacgaaac acaggcacag gagtttgtgc aggaagccct ggaaacgttt
    27181 cgctggcacc agtcagcaac ggtagatgaa gaaacctatc gcgccttgca caacgaacat
    27241 cggttgattg ctgatgtggt ctgtttccct ggatgccata tcaaccacct gacgccacgt
    27301 acgctggata ttgaccgggt gcagtcgatg atgcctgaat gcgggattga acccaaaatt
    27361 ctgatcgaag ggccgccgcg ccgcgaggtg ccgattttac tacggcagac cagctttaag
    27421 gcactggaag agacggtgtt gtttgcgggg cagaaacagg gcacgcatac cgcgcgcttt
    27481 ggtgaaattg agcagcgtgg cgtggcatta acgccgaaag ggcgacaact gtatgatgat
    27541 cttctgcgta acgctggaac cgggcaggat aatctcactc accaaatgca tttacaggaa
    27601 accttccgca cttttcctga cagtgagttt ttaatgcgtc agcaagggct ggcatggttc
    27661 cggtaccgtc tgacgccttc aggtgaggcg catcgtcagg ctattcatcc cggagacgat
    27721 ccacagccct taattgaacg tggttgggtc gcggcgcaac ccattaccta tgaagatttc
    27781 ttgcccgtta gcgcggcggg gatcttccag tcaaatctgg gtaatgaaac gcaggcacgc
    27841 agccacggta atgccagtcg cgaagcattt gagcaggcgt tgggttgtcc ggttttggat
    27901 gagttccagc tttatcagga agcggaagag cgcagtaaac gtcgctgtgg tttgctttaa
    27961 attctgacca tccgcctttg caaaaatttg cctgatttta caaacgaatc aggctcatcc
    28021 catcgacata aaaaaattgc cgatttatgc atattctctg agttcaacaa ttggattatt
    28081 aataaatatt gtctagagtg agcggtcata aataagcact ttcttgccgc tgaaaacgac
    28141 cagcgcggga ccattcacaa caccagaagg actcactttc aggtatggat cgtagacgat
    28201 ttattaaagg ttcaatggct atggccgccg tgtgcggtac cagcggcatt gcttctcttt
    28261 tttctcaggc ggcattcgcg gcagattctg atattgccga cgggcaaacc cagcgttttg
    28321 acttctccat tctacagtca atggcgcacg acttagcgca aacagcgtgg cgtggtgcgc
    28381 ctcgtccgtt acctgacacg ctggcgacaa tgacgccgca ggcttataac agtattcaat
    28441 acgacgccga aaaatcgctc tggcataacg ttgagaaccg tcaactggac gctcagttct
    28501 tccatatggg aatgggattc cgtcgccgcg ttcgtatgtt ttctgtagat ccagcaacac
    28561 atctggcgcg tgaaattcac tttcgcccgg agttgttcaa atacaacgat gcaggtgttg
    28621 ataccaaaca attagaaggg caaagcgatc tcggctttgc cggttttcgc gtgtttaaag
    28681 cccccgaact ggcgcgtcgt gatgtagtat cattcctcgg tgcgagttat ttccgcgccg
    28741 ttgatgacac atatcaatac ggtttgtcgg ctcgcggcct ggcgatcgac acttacaccg
    28801 acagtaaaga agaattcccc gactttaccg ccttctggtt tgatacggta aaaccggggg
    28861 caactacctt taccgtttat gcgttgctcg atagcgccag tattactggt gcctataagt
    28921 tcactatcca ttgtgagaaa agtcaggtga ttatggatgt ggaaaatcac ctgtatgcgc
    28981 gcaaagacat taaacagctg ggcattgcgc cgatgaccag tatgttcagc tgcggtacta
    29041 atgaacgtcg gatgtgcgac accattcatc cgcaaattca tgactctgat cgtctgtcca
    29101 tgtggcgggg caacggcgag tggatttgcc gtccgctgaa taatccgcaa aaattgcagt
    29161 tcaatgctta caccgacaac aacccgaaag ggtttggttt attgcaactg gatcgtgact
    29221 tctcccatta tcaggacatc atgggctggt ataacaaacg cccaagtctg tgggtggaac
    29281 cgcgtaacaa gtggggtaag ggcaccatcg gcctgatgga aatcccaaca acgggcgaaa
    29341 cgctggataa cattgtctgc ttctggcagc cagaaaaagc tgtaaaagcg ggtgatgagt
    29401 ttgcattcca gtatcgtctg tactggagtg cgcaaccgcc tgttcattgc ccattagcgc
    29461 gcgttatggc gacgcgtacc ggcatgggcg gtttcccgga aggttgggca ccaggtgaac
    29521 actatcccga aaaatgggcg cgtcgttttg ccgtcgattt cgttggtggt gatctgaaag
    29581 ctgccgcacc aaaaggcatt gagccggtga ttacgctttc cagtggggaa gcgaagcaaa
    29641 tcgaaattct ctatattgaa cccattgatg gttatcgtat tcagtttgac tggtatccga
    29701 cttcggactc cactgatccg gtcgatatgc ggatgtatct gcgttgtcag ggggacgcta
    29761 tcagtgaaac atggctgtat cagtatttcc cgccagcgcc ggataaacgt cagtatgttg
    29821 acgaccgcgt gatgagttaa tcgttttttc ttcggcacct tcttcgggag gtgccgtctg
    29881 gttaaacacg atcccgctcg catttttccc taagttaaat gagtaatctg atggtgtgta
    29941 tttcagatac accttgtcag ccactaacag ggagtgcgta tgtttccaga atatcgagat
    30001 ttaatatccc gtctgaaaaa cgaaaatcct cgctttatgt ccttgttcga taaacacaat
    30061 aaacttgatc atgaaattgc cagaaaggaa ggttccgacg gtcgagggta caatgcggaa
    30121 gtggtccgca tgaaaaaaca aaagctacag ttaaaagatg agatgctcaa aatcctgcag
    30181 catgagagcg tcaaagaggt gtaaactttc ctaagccgcc agccaggcgg cttttttaac
    30241 aactgcatgg attgactgga gataagatga ctgaaacgat aaaagtaagc gaatcacttg
    30301 aattacatgc tgttgcagaa aatcacgtca ctcctcttta tcagttaatc tgtaaaaata
    30361 aaacctggtt acagcagtcg ctaaactggc cgcagttcgt tcagagtgaa gaggacacgc
    30421 gaaaaacggt gcagggtaat gtgatgttgc atcaacgcgg ctatgccaaa atgttcatga
    30481 ttttcaaaga agatgaactt atcggcgtta tctcgtttaa tcgtattgaa ccactgaata
    30541 aaaccgctga aattggctac tggctggacg aatctcatca ggggcagggg atcatttctc
    30601 aggcgctgca ggcattgatt catcattacg ctcagtctgg tgaacttaga cgcttcgtga
    30661 tcaaatgtcg ggtggacaat ccgcaaagca accaggtcgc tttgcgcaat ggttttatcc
    30721 ttgaaggttg cctgaaacag gctgagttcc tgaatgatgc ctatgatgat gtgaacttat
    30781 acgcgcgtat tatcgattca caataaccct gccagcggcg ttcgcgtaat gcgctcctcg
    30841 ccgttaatga cttttggacc acggacatga accgtgtcgc catcaaaggc ttcaacgacg
    30901 gcgtggtcgg ttaactccac atggttttca attatcaccg cacctgtaat acggctctta
    30961 ccttgtatta caacgtgctc gtcgagcaga atcggtccgc cacgtaccac ggcatttccg
    31021 ccaatcaaga catgatgttt taacacacaa ttaccttcca caatggcata ttccgccacc
    31081 tgcgaactgt aatgaatcgt cggaatggca tcttcttcaa tgccagcttt cacctgcgcg
    31141 tgaccgtaga ctttagcgca atcgcatagc cagacgttgt tctcttcatt accttcaata
    31201 ctggcaaaat caaaaacttc ggcgcgatgt tcaataaaag cataccggat gacggcatcg
    31261 ccataaattt gtgcctggtg gacaatacgc gaggcgctaa cccttgcgcg atcataaatt
    31321 tgtagcagga gttgatggtc aggcgttaag ccttgtgctg cgacgatcat agaatgttga
    31381 ttaattaagg cgtgaccaaa tattctacat tgcccgcaaa ccagtgagtc atgaatggtg
    31441 acactgtcac ttatatatgc accctggctg atttcactat tatcgatcca gacattatcc
    31501 gttgcgtaaa cctctcccca cagcacgctg gtgccggtga tacgcgtatt accggaaatc
    31561 accgttccgc cgaacgcaat agcattctgg tcgtaaatcc agcaatttcc ttcctgtgct
    31621 aacaccgttt cgcgatcgat ccagccgcca gcggttcctg ctatcacatc gttaaaatcg
    31681 ctaatggcga taatctgccg taataacaca ctttttttag tgccatcttc ctgataacta
    31741 aaggctcgct gttcttcact tagacgatat ttgcgcatag cgttttccca caggtgactt
    31801 actataaccg tagcaaattc tgcggctctg gctatgctcg agaaaattcc ataaaatgca
    31861 tttcaaatat actttataaa ttaaacaaaa tgagtaagaa gatgcagagc gataaagtgc
    31921 tcaatttgcc ggcaggctac tttggtattg tgttggggac gatagggatg ggatttgcct
    31981 ggcgctatgc cagccaggtt tggcaggtca gccactggtt aggggatggg ttggtgattc
    32041 tggcgatgat catctgggga ttattgacta gcgcatttat tgcccgactc atacgctttc
    32101 cgcatagcgt gctggcggaa gttcgccatc cagtgctgag cagttttgtg agtttgtttc
    32161 cggcaacgac gatgctggtg gcgattggtt ttgttccgtg gtttcgccca ctggcggtgt
    32221 gcctgttcag ttttggtgtc gtggttcagt tggcttatgc cgcctggcaa actgcgggat
    32281 tatggcgcgg atctcaccct gaagaagcta ccacgcctgg actgtatctg ccgacagttg
    32341 ccaacaactt tatcagcgca atggcctgtg gtgcgttggg ctacaccgac gccggtctgg
    32401 tgtttttagg cgcaggcgtt ttctcatggc taagccttga accggtgatc ttgcagcgtc
    32461 tgcgtagttc gggagaatta cccacggcac tgcggacatc actcggcatt cagctcgctc
    32521 ctgcgctggt ggcttgtagt gcctggctga gcgtcaacgg cggcgagggt gacacgctgg
    32581 cgaaaatgct tttcggttat ggactgctgc aactgctgtt tatgctacgt ctgatgccat
    32641 ggtatctctc ccagccattt aatgcttcat tctggagttt ctcgttcggc gtatctgcac
    32701 tggcaaccac cggtttgcat ctggggagtg gcagcgataa tggatttttc catacgctgg
    32761 cggtgccgct gtttatcttt accaatttta ttattgcaat actgctcatc cgtacttttg
    32821 cgcttctgat gcagggaaaa ttgttagtca gaaccgagcg cgccgtttta atgaaagcag
    32881 aggacaaaga atgatcattc gtgacgaaaa ctattttact gataaatatg aattaacccg
    32941 cacacactct gaagtactgg aagcggtgaa agtggttaaa ccgggtaaaa cgctggatct
    33001 gggctgtggc aatggtcgta acagtcttta cctggcagcc aatggttatg atgttgacgc
    33061 atgggataaa aatgccatga gtatcgccaa cgtcgagcgc attaaatcta ttgaaaatct
    33121 ggataattta cacacccggg tcgttgatct gaataacctc acatttgctg gacagtacga
    33181 ttttattctt tcgactgtgg tgctgatgtt ccttgaggct aaaaccatcc cagggctgat
    33241 tgccaatatg caacgttgca ctaaacctgg cggttacaac ctgattgtgg cggcgatgga
    33301 taccgctgat tatccatgta ccgtcggctt cccgtttgcc ttcaaagaag gagaattacg
    33361 tcgatattac gaaggctggg agatggtgaa atacaatgaa gacgtcggcg agctgcaccg
    33421 caccgacgcc aacggtaatc gtattaaact gcgtttcgcc acgatgctgg cacgtaaaaa
    33481 atgacccggt aagaacaaag cgcgtgaaaa ttccccacgc tgagatggtt tactgtcctt
    33541 cttttcggta agcatatttt ttatcgaagg gatgtgaaat taatcacagt agtcgaagtt
    33601 ttttagcagc ttaacttact gaaatttaac tactgatgat tgacttagcc ccttttgcgg
    33661 cattgactat gtcgtctgaa aaggggctaa aaaaatttat tttcaccaac actttttttg
    33721 ccacaacacg aagcggcgct ttttgct
//
LOCUS       DAZFKF010000048        32960 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFKF010000048 DAZFKF010000000
VERSION     DAZFKF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.048"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(416..2104)
                     /gene="betA"
                     /locus_tag="ACHKA1_002738"
     CDS             complement(416..2104)
                     /gene="betA"
                     /locus_tag="ACHKA1_002738"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO5680366.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAM
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGSTE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGKDALPRSTAGYYVANGMPVRAKKMSRDLN"
     gene            complement(2118..3590)
                     /gene="betB"
                     /locus_tag="ACHKA1_002739"
     CDS             complement(2118..3590)
                     /gene="betB"
                     /locus_tag="ACHKA1_002739"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO5680367.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPVE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3604..4191)
                     /gene="betI"
                     /locus_tag="ACHKA1_002740"
     CDS             complement(3604..4191)
                     /gene="betI"
                     /locus_tag="ACHKA1_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO5680368.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4320..6353
                     /gene="betT"
                     /locus_tag="ACHKA1_002741"
     CDS             4320..6353
                     /gene="betT"
                     /locus_tag="ACHKA1_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO5680369.1"
                     /translation="MTDLSHSRENDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            6928..10899
                     /gene="ehaA"
                     /locus_tag="ACHKA1_002742"
     CDS             6928..10899
                     /gene="ehaA"
                     /locus_tag="ACHKA1_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO5680370.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LALSKDVAVSEDYTAVFGKDNQTIDYRISVTDNANLVIDATDSSRPRLTLASGGGLDI
                     TGGKVHINGSLNFLLKGTGFLNVANAGSELYADDLYESYSNITHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGY
                     YDQNSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     VPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGNTYYT
                     DMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDIAQWSHNGSDSWHV
                     GVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQYSWF
                     DNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTWMGVK
                     ADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLHNSKD
                     FSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAMVGIK
                     WQF"
     gene            11041..12129
                     /gene="pdeL"
                     /locus_tag="ACHKA1_002743"
     CDS             11041..12129
                     /gene="pdeL"
                     /locus_tag="ACHKA1_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5680371.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFVSEWVMKAGG"
     gene            complement(12171..13103)
                     /gene="yahB"
                     /locus_tag="ACHKA1_002744"
     CDS             complement(12171..13103)
                     /gene="yahB"
                     /locus_tag="ACHKA1_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5680372.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(13195..13692)
                     /gene="yahC"
                     /locus_tag="ACHKA1_002745"
     CDS             complement(13195..13692)
                     /gene="yahC"
                     /locus_tag="ACHKA1_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO5680373.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLCQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            13950..14555
                     /gene="yahD"
                     /locus_tag="ACHKA1_002746"
     CDS             13950..14555
                     /gene="yahD"
                     /locus_tag="ACHKA1_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO5680374.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQVLIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            14595..15458
                     /gene="yahE"
                     /locus_tag="ACHKA1_002747"
     CDS             14595..15458
                     /gene="yahE"
                     /locus_tag="ACHKA1_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO5680375.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     HDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFRLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRFRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            15448..16995
                     /gene="fdrA"
                     /locus_tag="ACHKA1_002748"
     CDS             15448..16995
                     /gene="fdrA"
                     /locus_tag="ACHKA1_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO5680376.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETSVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIDAI
                     KEAKAIAAAGGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            16995..18413
                     /gene="yahG"
                     /locus_tag="ACHKA1_002749"
     CDS             16995..18413
                     /gene="yahG"
                     /locus_tag="ACHKA1_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO5680377.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFEQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVAQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            18439..18693
                     /locus_tag="ACHKA1_002750"
                     /pseudo
     CDS             18439..18693
                     /locus_tag="ACHKA1_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18743..19693
                     /gene="yahI"
                     /locus_tag="ACHKA1_002751"
     CDS             18743..19693
                     /gene="yahI"
                     /locus_tag="ACHKA1_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO5680378.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTEVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STVLLAREIHADILVITTGVEKVCIHFGKPEQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPESLPAALRGETGTHIIKT"
     gene            19703..21085
                     /gene="yahJ"
                     /locus_tag="ACHKA1_002752"
     CDS             19703..21085
                     /gene="yahJ"
                     /locus_tag="ACHKA1_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO5680379.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            21298..21375
                     /locus_tag="ACHKA1_002753"
                     /pseudo
     CDS             21298..21375
                     /locus_tag="ACHKA1_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21462..22511
                     /gene="yahK"
                     /locus_tag="ACHKA1_002754"
     CDS             21462..22511
                     /gene="yahK"
                     /locus_tag="ACHKA1_002754"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO5680380.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            22754..23569
                     /gene="yahL"
                     /locus_tag="ACHKA1_002755"
     CDS             22754..23569
                     /gene="yahL"
                     /locus_tag="ACHKA1_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO5680381.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQVLAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            23984..24229
                     /gene="yahM"
                     /locus_tag="ACHKA1_002756"
     CDS             23984..24229
                     /gene="yahM"
                     /locus_tag="ACHKA1_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO5680382.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAWIRQIRLIT"
     gene            complement(24246..24917)
                     /gene="yahN"
                     /locus_tag="ACHKA1_002757"
     CDS             complement(24246..24917)
                     /gene="yahN"
                     /locus_tag="ACHKA1_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO5680383.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            25064..25339
                     /gene="yahO"
                     /locus_tag="ACHKA1_002758"
     CDS             25064..25339
                     /gene="yahO"
                     /locus_tag="ACHKA1_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO5680384.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTADIYKKK"
     gene            complement(25440..27026)
                     /gene="prpR"
                     /locus_tag="ACHKA1_002759"
     CDS             complement(25440..27026)
                     /gene="prpR"
                     /locus_tag="ACHKA1_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO5680385.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLLRYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            27265..28155
                     /gene="prpB"
                     /locus_tag="ACHKA1_002760"
     CDS             27265..28155
                     /gene="prpB"
                     /locus_tag="ACHKA1_002760"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO5680386.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARNQVK"
     gene            28315..29484
                     /gene="prpC"
                     /locus_tag="ACHKA1_002761"
     CDS             28315..29484
                     /gene="prpC"
                     /locus_tag="ACHKA1_002761"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO5680387.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSAIGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            29518..30969
                     /gene="prpD"
                     /locus_tag="ACHKA1_002762"
     CDS             29518..30969
                     /gene="prpD"
                     /locus_tag="ACHKA1_002762"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO5680388.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPTFT
                     ADYHDPEKRAIANAVTLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            31009..32895
                     /gene="prpE"
                     /locus_tag="ACHKA1_002763"
     CDS             31009..32895
                     /gene="prpE"
                     /locus_tag="ACHKA1_002763"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO5680389.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
BASE COUNT         8080 a         8214 c         8931 g         7735 t
ORIGIN      
        1 agcgaagcga taatcttgtg ctaaaaaata tttaagcatg atgattaatt aaagaagagt
       61 aaaaataaat cgctgctaat aaattatacg aaccgtgttt aaagctccat gtggttttgg
      121 tctgacttat ttttgtactc gttacaagaa cccttttcgt acatgctaac cgtgagcggg
      181 aatgaacagg taaccatagc aaggaacatt agcggcgtaa ttcttaagct gagtggtatt
      241 tcatctgtag tttatctgtg tatgcatagc accttcatca gccagattga gcaagtcagc
      301 ttagaatttg ctggcgaggt tgtcactatg aagcatgaga gttaccccta tgttttatat
      361 aacgattctg tccccatacc tggaacggta actctcaatc tgatcggttc ctgagtcagt
      421 tcaaatcacg actcattttt ttcgctctca ccggcatccc atttgccaca taatatcccg
      481 ccgtgctcct cggcaatgca tccttcccgc gaatcatatc cgctattttc tcgccaatca
      541 taatcgtcgt ggcgttcaaa ttgccggtga taatctgcgg cataattgac gcatccacca
      601 cgcgcaggcc ttccagccca tgcacgcggc cttcgccgtc aaccactgcc atctcgtcat
      661 aacccatttt gcaggtaccg cacgggtgga aggcggtttc ggcgtgatta cgcacgaact
      721 catcgagctg ctcatccgtc tgacattccg tgccggggct gatttcgcgg ccacgatact
      781 gatccagcgc cggttgatgc atgatctcgc gggtgatgcg aattgcgtcg cggaactcct
      841 gccagtcctg ctcgtgcgac atgtagttaa acagaatcgc cggatgctgg tgcgggtcgc
      901 gggatttaat ccgcacatgc ccacggcttg gcgagcgcat cgatccgacg tggcactgga
      961 agccgtgctc tttcactgca ttcgagccgt tatagttaat cgctactggc aggaaatggt
     1021 actgaatatt cggccacgca aattcctctc ggctgcgaat aaatccgcct gcttcaaagt
     1081 ggttgctggc accaacgccg gtgccgccaa acagccactc cgcaccgatt ttcggctgat
     1141 tccaccactg cagggcaggg tagagggaaa ccggttcttt gcactcatat tgcagataca
     1201 tctccaggtg atcctgaaga ttttcgccga cgccgggtaa atcatgcacc agcggaatat
     1261 caaactccgc cagcagttca gtgctaccca cgccggaacg ttgcaggatc tgcggagagg
     1321 cgatcgcacc tgcacataac agcacctctt tattcgctgt cgcgcgggtt ggaatggtgc
     1381 tatcgccttc cagccactcg acgcctaccg cgcgtttgcc gtcaaaaatg atgtgatcgg
     1441 tcatagcgtg agtacgaatg gtcaggttcg ggcgcgattt ggcctgatcg agatagccgc
     1501 gcgcggtgct ggcgcgacgg ccctgcggcg tcacggtgcg atccatcggg ccaaaacctt
     1561 cctgctgata gccgttgaga tcgtccgtgc gcgggtagcc cgcctgcacg cccgcttcaa
     1621 tcatcgcttc aaacagcgga ttgacgccgg gtttggaggt ggtgacgctc accgggccgt
     1681 cgccgccgtg gtagtcgttc tcgcccacgt cgcgggtctc ggccttgcgg tagtagggca
     1741 ggcaatcgag ataactccag ttctccagac cgggttcttt tgcccagttg tcgagatcca
     1801 tcgcgttgcc acggatgtag cacatgccgt tgatcagcga cgatccaccc aggcctttac
     1861 cgcgtccgca ctccatgcga cggttattca taaacggttc aggttccgtc tcgtaagccc
     1921 agttgtagcg tttaccctgt agcgggaatg ccagggcagc gggcatctgg gtgcggaagt
     1981 caaagcgata gtccgggccg cccgcttcaa gcagcagcac ggaggtattc ggatcttcag
     2041 tcagacgggt agcgagaacg ttgccggctg agccggcacc aataatgatg tagtcaaatt
     2101 gcaaataaac ctcctggtta gaatatggac tggaatttag ccatctcaac ctggatggac
     2161 ttcacctggg tgtaactctg gagcgtcatc acgccgttct cgcgaccaat gccggagtgt
     2221 ttgtagccgc caacgggcat ctctaccggg gattcgcccc aggtgttgat ccagcaaata
     2281 cccgcttcca gctgatgaat gacgcgatgc gcgcggttca ggtccgccgt cacgattccc
     2341 gccgccaggc cgtagtcggt atcgttagcg cggcgaatga cttcgtcttc cgactcgtag
     2401 gtcagaatgg acatcactgg cccgaagatc tcttcacgca cgatggtcat atcgtcgctg
     2461 caatcggtga acacagtcgg cgcgacccat gcgccgttat cgaggccatc gcctttcagt
     2521 acatcgccgc cgcacagtac gcgcgcgcct tcctctttgc ctttggcgat atagcgcagc
     2581 acgttatcgc gatgcgggaa gctgaccagc gggccgaagt tagtttgcgg atcgaaaacg
     2641 tcgcccgcgc gaatgcgctc aacgcgcgcc agaatctttt gttcaaatgc ggctttgcat
     2701 ttcgccggaa cgaagacgcg ggtgccattg gtacacacct gaccggagct gaagaagttt
     2761 gccatcatgg cgatatcggc ggcgagatcg agatccgcat catcgaaaac gatcagcggt
     2821 gatttaccgc ccagttccat ggtcacttct ttcagggaag aggccgccga gttagccatc
     2881 acttttttgc cgctggcgac accgccggta aatgacactt tggcaatgcc cggatgctcg
     2941 gtcagatatt gcccggtctc cgcgcccacg cccggcaaca cgttaaatac gccgtccggc
     3001 aggcccgctt cgctgtaaat ttcagccagc tttaacgcgg taagcggggt gacttcgctc
     3061 ggtttgaaaa tcattgcgtt gcctgccgcc agcgccgggg cggatttcca cagggcaatc
     3121 tggatcgggt agttccatgc gccaatcccc gccaccacgc ccagcggttc gcggcgggta
     3181 taaacaaatg acgtctcacg caacgggatc tggctgcctt ccagcgccgg gatcagcccg
     3241 gcgtagtact ccagcacgtc cgcaccggta acgatatcga cggttgaggt ttccgagtat
     3301 gcttttccgg tgtcgagggt ttccagtttt gcgagttcgt cattgcgttc acgcagaata
     3361 tcaacggccc gacgcagaat acgcgagcgt tccatagcgg tcatcgccgc ccagattttt
     3421 tgcccctgtt gggcactctt cacggcgcga tcgacatcct cacgcccggc ggcctgcacg
     3481 gtcgccagca cgttaccgtt ggccgggtta atggtctcga aagtgcgacc gctggtggcg
     3541 gaggtataac caccatgtat ataaagctgc tgttctgcca ttcgggacat cacgtctcct
     3601 cggttaatcg gtgggtaaat gctgagtaat aaagtgacgg gtcagcgagt gtgcgagagg
     3661 tttatccagc ggtttgccgc tcagtgccgc gcgcagccat aatccgtcaa tcagcgcggc
     3721 aaggccgtag cccgcttcct gtgcctggtg gcgcggcaat tcgcggcgaa actcgctcac
     3781 cagattcgac agcaagcggc ggctgctgac ctgctgtaaa cgatagagca tcggctgatg
     3841 catactgctg gcccagaacg ccagccaggc tttcatcgcc gcactgctca cctgcgtttc
     3901 atcgaagttt ccgccaacaa tcgcctgtaa tcgctgctct gcactgccct gcggaagtgc
     3961 atgtaatcga ttcaaaaccg cgtcacgtag ctgactggtg atatcgcgca tggttgcttc
     4021 cagcagacca tttttgtccc tgaaatagtg gctgatgatc cccgtagaaa cgcctgcacg
     4081 gcgggcgatc tgcgcgatcg ttgcatcgtg catgcccact tcatttattg cttccagtgt
     4141 ggcgtcgatc agttgtctgc gccggatcga ctgcatcccc aatttgggca ttttcgccac
     4201 tccattcatc agcgttgttt atctattaaa gcggttattg attggacgtt caatataaaa
     4261 tgtgtcttaa ttgttacgaa tttgatttta aatggtaaca ataacagtgg ggatactgga
     4321 tgacagacct ttcacacagc agggaaaatg acaaaatcaa tccggtggtg ttttacacct
     4381 ccgccggact gattttgttg ttttccctga caacgatcct gtttcgcgac ttctcggccc
     4441 tgtggattgg ccgcacgctg gactgggttt ctaaaacctt tggttggtac tatctgctgg
     4501 cggcaacgct ctatattgtc tttgtggtct gtatcgcttg ttcgcgtttt ggttcggtga
     4561 agctcgggcc agaacaatcc aaaccggaat tcagcctgct gagttgggcg gcgatgctgt
     4621 ttgctgccgg gatcggtatc gatctgatgt tcttttccgt agccgaaccg gtaacgcagt
     4681 atatgcagcc gccggaaggt gcgggacaga cgattgaggc cgcgcgtcag gcgatggtct
     4741 ggacgctgtt tcactacggc ttaaccggct ggtcgatgta tgcgctgatg ggcatggcgc
     4801 tcggatactt tagctatcgt tataatttgc cgctcaccat ccgctcggcg ctgtacccga
     4861 tcttcggtaa acggattaac gggccgatag gtcactctgt ggatattgca gcggtgatcg
     4921 gcactatctt cggtattgcc actacgctcg gtatcggtgt ggtgcagctt aactatggct
     4981 tgagcgtact gtttgatatt cccgattcga tggcggcaaa agcggcactg atcgccttgt
     5041 cggtgataat cgccactatc tcggtgacat ccggtgtcga taagggcatt cgcgtgttat
     5101 cggagcttaa tgtcgcgctg gcgctgggat tgatcctgtt cgtgttgttt atgggcgaca
     5161 cttcgttcct gcttaatgca ctggtgctga atgttggcga ctatgtgaat cgctttatgg
     5221 gcatgacgct caacagtttt gccttcgacc gtccagttga gtggatgaat aactggacgc
     5281 tcttcttctg ggcatggtgg gtggcatggt cgccgtttgt cggcttgttc ctggcgcgta
     5341 tctcgcgtgg gcgtaccatt cgccagttcg tgctgggcac gttgattatt ccgtttacct
     5401 tcacgctgtt atggctctcg gtgttcggca atagcgcgct gtatgaaatc atccacggcg
     5461 gcgcggcatt tgcagaggaa gcgatggtcc atccggagcg cggcttctac agcctgctgg
     5521 cgcagtatcc ggcgtttacc tttagcgcct ccgtcgccac cattactggc ctgctgtttt
     5581 atgtgacctc ggcggactcc ggggcgctgg tgctggggaa tttcacctcg cagcttaaag
     5641 atatcaacag cgacgccccc ggctggctgc gcgtcttctg gtcggtggcg attggcctgc
     5701 tgacgctcgg catgctgatg accaacggga tatccgcgct gcaaaacacc acggtgatta
     5761 tggggctgcc gttcagcttt gtgatcttct tcgtgatggc ggggttgtat aaatctctga
     5821 aggtagaaga ttaccgccgt gaaagtgcca accgcgatac cgcaccgcga ccgctggggc
     5881 ttcaggatcg cctgagctgg aaaaaacgtc tctcgcgcct gatgaattat ccgggcacgc
     5941 gttacactaa acagatgatg gagacggtct gttacccggc aatggaagaa gtggcgcaag
     6001 agctgcggtt gcgcggcgcg tatgtggagc taaaaagcct accgccggaa gagggacaac
     6061 agttggggca tctggatttg ttggtgcata tgggcgaaga gcaaaacttt gtctatcaga
     6121 tttggccgca gcaatactcg gtgccgggct ttacctaccg cgcacgtagc gggaaatcga
     6181 cctactaccg gctggaaacc ttcctgttag aaggcagcca gggcaacgac ctgatggact
     6241 acagcaaaga gcaggtgatc accgatattc ttgaccagta cgagcggcac cttaacttta
     6301 ttcatctcca tcgtgaagcg ccgggccata gcgtgatgtt cccggacgcg tgattgttat
     6361 tgcataaaac cgcgccatgt ctgcatatgg cgcgggaatc attgactcgc tgtaaaaaat
     6421 tatgcatata ttatatatga ttgtcatgtc atatcctgaa attcctcttg tatcacttca
     6481 tgattttata ttttattaat taacgatttt attttgtatt agctgaatgg attcagtcct
     6541 atagggttat atatttatta ataagacgat tcttatattt ggatacctaa taagagttac
     6601 cacttttacc ggctgtatat taaatgtatt tctcctgctt ttgtttatat tttgagttga
     6661 tttgagttcc cggatacata taatgcgcag gtattagaga ctgggggggg aatgtaatga
     6721 taaagttaag agtgttaact taaaataagt tttcagtagt aacttgctat tttattaccc
     6781 tctgaactac atactcagat atgtggttgc caatgctatg caaaattttt gaatttttat
     6841 atgcgatgaa cagtaaccgg aatggcattt aatgcgttgc tgtttatgca atcttggttt
     6901 tatttggatg tattgctgga gattgtgatg aataaaatat atcggctaaa gtggaacagg
     6961 tcccgtaact gttggagcgt ctgctctgag ctggggagca gagtaaaagg aaaaaagtcc
     7021 cgggctgttt taattagcgc gataagttta tattcatctc tggcactgtc taaagatgtc
     7081 gctgtaagtg aagattacac agctgtattc ggtaaagata accagactat tgattaccgt
     7141 atttcagtga ccgacaatgc caatctggta atcgatgcga cagattcttc tcgtccacgt
     7201 ctgactctcg cgtctggtgg tgggctggat atcactggag ggaaggtgca tatcaatggc
     7261 tcgcttaact ttttgttaaa agggacgggg tttctgaacg tcgccaatgc tggcagcgag
     7321 ttatatgctg atgatttata tgagtcttac tctaatatta cccacgatag aggctatttt
     7381 aatgtctcca acggcggcaa aatccatgtt aagggcacca gccgtctgac ctatttgcag
     7441 ggaaatgtca gcggtgaagg cagccaggta aattctaaaa ccttctttat gggcgtttac
     7501 ggcagttacg gtggtaatca gtacctgtca gttaataacg gcggtgaagt taatgccagt
     7561 gagaaaatta gcctgggtta ttatgatcaa aactccgata caacacttgc cgtttcggaa
     7621 ggtggtaaaa tctctgcgcc gactattagt ttaagcacca actctgagtt agcgttaggt
     7681 gcacaggaag gaagcgcagc gaaggctgca gggattattg atgccgaaaa aattgagttt
     7741 gtgtgggcaa agacatccga gaagaaaatc accttaaacc acacggataa aaacgcgact
     7801 atttccgcgg atattgtcag tgacagcgag ggcctgggct atatcaatgc gctcaatggt
     7861 acgacttact taaccgggga taactccgcc tttagcggta aagtcaaaat tgagcaaaat
     7921 ggcgctttag ggatcaccca aaatataggt acagcagaaa tcaacaaccg cgggaaatta
     7981 cacctgaagg ctgacgatag catgaccttt gccaataaga tttccggcaa tggtacaata
     8041 agtatcgaca gtgggacagt ggcactgacc ggcaataact atgcattcag cggatatatt
     8101 gatgtcgctt ctggtgctgt cgcggttatt tctgaagaca agaacatcgg ccgtgcggac
     8161 ctggatgttg atggcaaact gcaaatcaat gccaataaag attgggtatt tgataacgat
     8221 cttgaaggta gaggtattgt tgaaataaac atggggaatc acgaattctc cttcgatgag
     8281 tttgcttata cagactggtt ccagggttca ctggcgttcc agaacacgac atttaatctg
     8341 gaaaagaatg ctgagtttct gcagaaaggc gggatcactg cgggtcaggg aagcctggta
     8401 acagtgggta agggcgctca ctccattagc actttgggat tctccggcgg aaccgttgat
     8461 tttggtgccc tgacagcagg tgcacagatg acagaaggga cggtcaacgt tagtaaaacg
     8521 ctggatttgc gcggcgaggg tgtgattcag gtttctgaca gtgacgttgt aagctcagta
     8581 tctcgtgata ttgactctgc gttatcgctc actgaagtcg atgatggtaa cagcaccatt
     8641 aagttggttg atgcgcaagg tgcggaagtt ctgggcgatg cgggcaatct gcaattgcag
     8701 gataaaaatg ggcaaatcct ctccagcagc gcccaacgtg atattcagca gaatgggcaa
     8761 aaagcggccg tcggcactta cgactatcgt ctgacgagtg gggtaaacaa tgacggtctg
     8821 tatatcggtt acggcctgac ccagcttgat ttacacgcta ccgacagcga tgctctggtg
     8881 ctgagctcta acggtaaaag cgagaatgcc gccgatctca gcgcaaagat taccggcagt
     8941 ggtgacctgg cattcagcag ccagaagggt cagaccgtat cgctttctaa caaagacaac
     9001 gactataccg gtgttaccga tctgcgcagt gggacgcttt tgttgaataa cgataacgtg
     9061 ttgggtaata cccatgaact gcgtctggcg gcagagactg aactggatat gaatggtcac
     9121 agccagaccg tgggcacgct caatggcagc gccgattcac tgctgagctt aaatggcggc
     9181 agtctgacgg ttaccaacgg gggcacttca accggttcgt taacggggag cggagagctg
     9241 aatattcagg gcggcacgct ggacatcgcg ggcgataaca gcaacctgac ggcgaatgtg
     9301 aacattgcta attcggctaa tgtcctggta agtcatgcgc agggattggg tagcgcaaac
     9361 gttgagaaca acggtaccct ggcgttgaat aatagcgctg aaaaaagagc ggctgcgtct
     9421 gtgaattaca ccctgggcgg caatctgacc aacaacggta cgctgatgac cggaatgtca
     9481 ggacagcaag ctggcaatgt gttagtggtg aaggggaact accacggtaa taacggtcaa
     9541 ctagtaatga atacggtact gaatggcgat gactcagtaa ccgataaatt ggttgtcgag
     9601 ggcgatacta gcggcacgac tgccgttacg gtgaataacg ctggcggtac aggtgcgaaa
     9661 acccttaacg gtatcgaact tatccatgta gacggtaagt ctgagggcga atttgttcag
     9721 gctggtcgta tcgttgcggg ggcgtatgac tacactctcg cgcgtggaca aggggcaaat
     9781 agtggtaact ggtatctgac cagcggcagt gattctcctg aactgcagcc ggagccagac
     9841 ccggtgccga atccgaatcc agagccgaac cctaacccga cacctacgcc gggcccggat
     9901 ctgaatgtgg ataatgacct gcgaccggag gcgggtagct acattgcgaa ccttgcagca
     9961 gcgaatacca tgttcaccac gcgtctgcat gagcgtctgg gtaatacgta ctataccgac
    10021 atggtgacgg gtgagcagaa acaaaccact atgtggatgc gccatgaagg tggtcataat
    10081 aaatggcgtg atggcagcgg ccagctgaaa acccaaagca atcgctatgt tctgcaactg
    10141 ggaggcgata tcgcgcagtg gagccataac ggcagcgaca gctggcatgt tggggtcatg
    10201 gcgggatatg gcaacagcga cagcaaaacc atttcctcgc gaaccggtta tcgtgcaaaa
    10261 gcgagtgtga acggatatag cacaggcctc tatgccacct ggtatgccga tgacgagtcg
    10321 cgtaatggcg cgtatctcga cagttgggcg cagtacagct ggtttgataa cacagtgaaa
    10381 ggggatgact tacaaagtga atcctataaa tcaaaaggat ttaccgcttc actggaagct
    10441 ggatacaaac acaaattagc tgaatttaat ggcagccagg gaacgcgtaa tgaatggtat
    10501 gttcagccgc aagcacaggt tacctggatg ggagtcaaag ccgataagca ccgcgaaagc
    10561 aacggaaccc tcgttcatag caacggtgat ggcaatgttc aaacccgact tggcgtaaaa
    10621 acctggctga agagccacca taaaatggat gacggtaaat cccgcgagtt ccagccgttt
    10681 gtagaagtga actggctgca taacagtaag gatttcagca ccagtatgga tggcgtgtcc
    10741 gtcactcagg atggagcccg aaatattgct gagataaaaa ccggggtgga aggacagcta
    10801 aatgccaacc tgaatgtctg ggggaatgtg ggcgttcagg ttgccgatag gggatataat
    10861 gacacctctg caatggttgg cattaagtgg caattctgat ctgaataatg agtaaaagga
    10921 gagagagccg aacgggagat aattcggctc tctgtgtcat tttcttgcag ccagctttat
    10981 ccgtcagagt gaagactcgc agatttagct ggcgcacaag atttaatcaa ggtgtgttca
    11041 atggcttcgc atgatttgag tgtttttttg gaagagttcg gggcaacggt gaatttgacg
    11101 ttgcctggta tcgtgtccga aaaagaacgg cttttactca agctgctaat ggagggaatg
    11161 tcggttacag aaatctcaca gtacagaaat cgcagtgcaa agacaatttc acatcaaaag
    11221 aagcagctgt atgaaaaact aggcatccag agtgatatta ccttctggcg ggatatcttt
    11281 tttcaatatc atccgcaagt catcagtggc acgggaaata aaaataattt ttatatccct
    11341 gataaccgtt gtcaccatat cgtcacgcct gaagccatca gtctggcgct ggaaaaccac
    11401 gaattcaaac cgtggatcca accggttttc tgcgcgcaga ctggggtact gacgggctgt
    11461 gaggtgcttg tccgctggga acatccacaa acgggaatca tcccgccgga tcagtttatt
    11521 cctctggcgg agtcatccgg tcttattgtc ataatgaccc gccaactgat gaaacagact
    11581 gcggatattc tgatgccggt aaaacatttg ctgccggata atttccatat tggtatcaac
    11641 gtctcggcgg gttgtttttt ggccgcggga tttgaaaaag aatgcctgaa cctggtcaag
    11701 aaattaggaa acgataaaat caaactggtt ctcgagctaa cggaacgtaa ccctattccg
    11761 gtaacgccag aagccagagc gatatttgac agccttcatc agcataacat tacctttgcg
    11821 ctggatgact ttggtacggg atatgcgacc tatcgttatc tgcaggcgtt tccggtcgat
    11881 tttattaaga tcgataagtc atttgtgcaa atggcgagtg tcgacgaaat atccggtcat
    11941 attgtggaca atattgtcga actggcgcgt aagcctggtc tgagtatcgt ggcggaaggg
    12001 gtagaaaccc aggagcaggc ggatttaatg atcggtaaag gcgttcactt tttgcagggc
    12061 tatttgtact ctccgccagt accgggtaat aaatttgttt ctgaatgggt aatgaaagct
    12121 ggtggttgat gtaaaccgct attcacagcg catcgggagg ttggcagcga ttaattctcc
    12181 cgatgcagta aatcctgata gatccccgtt aatatgccat ttggcgcaaa ttgcttttta
    12241 atccactgcg tcacctgacc cgtagcagaa tgctgagtcg ccagtaacat gcgcgaatcc
    12301 tggcgcggat tatggatttg tcgggtaacc agcagggatt gcgtcatcgc ctcacggacc
    12361 atgtaatccg gcaaaaaacc aatcccttca cctaaaatct ggcactgaca tttggtgttg
    12421 aaatctggca ccagaattga ctcctgcccg tgcagcaacc agcccacttt tttattaatc
    12481 gtatgcgcgg tgtcctccac cataatatta gggtagagac gcagttggct ttcggcgatg
    12541 ggttccggca caaaggctaa cgggtgatcc ggggcgatag caaaagccca gcgaatcgcg
    12601 ccaatttcag tgtaatcaat accgccgccg tccagtagtg tatcgggtgc gccgatggcg
    12661 atattggcct gattattaat aatcgcatcc cagacgccgt tatacacttc ggtcgtcacg
    12721 gtaatctggc aggtaggaaa ctgctttttc agcacctgta ataagcgcgc ggtgtgtttg
    12781 ggcgtataaa gcagctgatt aatacaaatg cgcacacgcg cttctatgcc ctgcgaaata
    12841 gtatcgatac tgcgtttgat ggcgtgaaaa tcattcagca ggtcggtagc tttgcggaaa
    12901 aaataacgcc cggactccgt taactcaatg ctgcgggtac tgcgagtgaa cagcaccaca
    12961 tccagccccg tctccatacg cttaatggtg tagctaatgg cggaagtggt taaacccaac
    13021 tcttctgcgg ctttactgaa actgccaaaa cgcgcagcgg tagtaaatgc caacagattc
    13081 tcttcggtaa aaattgagtt cattaaacca ttcccgccat tatctactat gaatttctcc
    13141 agctatgact gtagaggtat cggttaaaga tagataatca tttttgaata acttttaata
    13201 cccgtcgcgt ttagtcgtcg cttcgcttaa tttttgtcca ccatattctg aaattatccc
    13261 cacgcagtta cccgccagaa aacccagtaa cgtcaccgtc cagttcatcc cgctggcaaa
    13321 aaacagcgtc atgccgagaa agccaccggg aataaatgac agtaaccaga aacgcccttg
    13381 ccagaccacc accgccgaga agggtacggt agtaattacg ctcgcccaga acgcggacag
    13441 tccacttaca gaggccagcc aaccggcagc cagcgcggcg aaaaacgccc acaccatccc
    13501 ggaataatta accgccaggc ttctgataaa ccccgattta ccgccgcctg cggcataaaa
    13561 actgcaaaag gcgacgaagc caatggttcc taataattcc catccctgac agagcccgac
    13621 atgtgaagac actaactgcc aaagccctgc acaaataccg acggtgacac ccgttgccgt
    13681 gagtccgttc atcttgttcc cctctggtta gacatggacg aaagctgcgc ttatatgtgc
    13741 agactataga gaaataaaac ctgattgagt gaaagggtat cgggtcaaag aaataaatat
    13801 tgcactatcg cacactgcga aaagattgtt gaacatcatt caacaaaaag gcgaaatacg
    13861 gcgaattaaa tttaagcggg ttcttttatc agattatcgt tgctattctc aattcatcga
    13921 aaacagccac aaaaaccagg ggaatgtgaa tgagtataaa aaatctacct gccgactatt
    13981 tattggctgc acaacagggt gatatcgata aagtaaaaac ctgccttgcg ctgggtgtcg
    14041 atattaatac ctgcgatcgt caggggaaaa cggcaattac gctggcaagt ttatatcagc
    14101 aatatgcttg cgttcaggta ttaattgacg ccggagcgga tattaataaa caagatcata
    14161 cctgtttaaa tcctttttta attagctgtc tgaacgatga tttaacgcta ctacgaatta
    14221 ttttaccggc taaacccgat cttaattgcg taacccgttt tggcggtgtc ggcctgacgc
    14281 ctgcctgtga aaaaggccat ttaagtattg taaaagaact tttggcacat acggagatta
    14341 acgttaacca gaccaaccat gtaggctgga cgccgctact ggaagcgatt gtgcttaatg
    14401 atggtggtat taaacagcag gcgattgtcc agttattgct ggaacacggg gccagcccgc
    14461 acatgacgga taaatatggc aaaacgccgc tggaactggc gcgggaacgg ggctttgaag
    14521 agattgcgca gttactgatt gccgcaggtg cataaaccgg gaggcttgct atcaacacac
    14581 cagaaaaacg gtgtgtgtgg gcgctaactg cggatgcgga ttttctggcg cagcgggggc
    14641 aaggacaggt tgaacaggtc tttgccagag cggtaaatat cgcactcccg gctcgccagc
    14701 agttgctgac gctgctttgt gaagagcacg acaatgcgcc aaacagttgt cggttggcac
    14761 tcactcactt tgatgatctg ttccggcatg gtgataaggt tcagtttgac gatcaaggta
    14821 ttacggttgg tcaacatctt catatagaga tgagtcgttg tcggcgttgg ctgtccccaa
    14881 ccttgcaaat gaccgctgtg aattttcgcc ttatcgcctg gcaacagtgg cacgacatca
    14941 ttcatcagca cctgggggaa aatgaaaccc tgtttaatta tcgcggcgat aatccgtttt
    15001 atcaggcgtt aaataaagaa ttacatatta aacgacgggc agttatacag gcagtaatcg
    15061 ataaacaaaa tatcgcctca gcggtcgcca gtatgatggg cttagggatt ggccttacgc
    15121 catcagccga tgattattta acaggtctgg cacttatttt atttatttcc gggcatccgg
    15181 cgggaaaata caaagaggaa ttttatctcg gcttgcaacg cggcagaaat aataccacgt
    15241 tattaagtgc cataacgctg gaagccgcat tacaacaacg cttccgggaa aatattcatc
    15301 gttttattca caacattatt tatgacatcc ctgggaacgc aactcaggca atagaaaaaa
    15361 ttaaacatat tggctccagt tccggctgcg acatgctgta tggcatggcc gatggttgtg
    15421 cgctgagcca aacctacgga gggaattatg tcagttaaaa tagtcattaa accgaatacc
    15481 tattttgatt ctgtctcgct gatgtctatc tccacgcgtg caaataaact cgacggcgtc
    15541 gagcaggcat ttgtggcgat ggcgaccgaa atgaacaaag gtgtgctgaa aaatttagga
    15601 ctgctgacgc cggagctgga acaggcgaaa aacggcgacc tgatgattgt catcaatggt
    15661 aaatcggggg cggacaacga gcagttgctg gtggagattg aagaactgtt caacagcaaa
    15721 gcgcaaagcg gctcgcacga ggcgcgttac gccactattg ccagcgccaa aaagcatatc
    15781 ccggaaagta acctggcggt gatttcggtc aacggtctgt ttgccgctcg cgaagcgcgt
    15841 caggcgctgc aaaatgacct caacgtgatg ctgttttccg ataacgtctc agttgaagat
    15901 gaactggcgc tcaagcaact ggcccacgaa aaagggctgc tgatgatggg gccagactgt
    15961 ggcacggcga ttatcaacgg cgcggcgctc tgttttggta acgccgtgcg tcgcggcaac
    16021 atcggtattg ttggcgcatc cggcaccggc agccaggagc tgagcgtccg cattcatgaa
    16081 tttggtggcg gcgtttcaca actgattggc accggcgggc gcgacctgag cgagaaaatc
    16141 ggcggcctga tgatgctcga cgccatcggg atgctggaaa acgatccgca aactgaaatc
    16201 attgcgctga tctccaaacc gcctgcgcct gcggtggccc gcaaagtgct ggaacgtgcg
    16261 cgcgcctgcc gcaagccggt ggtggtctgc ttcctcggtc gtggcgaaac gtcagtggat
    16321 gagcaggggc tacagtttgc tcgcggcagc aaagaagcgg cgctaaaagc ggtgatgctc
    16381 tccggcgtga aacaggaaaa tctcgaccta catacgctta accagccgtt gattgcggat
    16441 gtgcgtgcgc gtctgcaacc gcagcagaaa tacattcgtg gcctgttctg cggcggcacg
    16501 ttgtgcgacg aaaccatgtt cgcggtgatg gaaaaacatg gcgatgtcta cagcaacatt
    16561 cagcccgatc cggaattccg cctgaaagat atcaaccgca gcatcaaaca caccttcctc
    16621 gactttggcg atgacgactt caccaacggc aagccgcatc caatgattga ccccaccaac
    16681 cgcatcagtc gcttgatcga agaagcgcgc gatccagaag tggcggtgat cgtgatggat
    16741 tttgtgctcg gatttggatc gcacgaagat ccggttggtt ccaccatcga tgcgatcaaa
    16801 gaagcaaaag cgatcgccgc agccggaggg cgcgagttga tcattctcgc ctatgtgctg
    16861 ggcaccgatc tcgatacgcc atcgttagaa caacaaagcc agatgctgct tgatgccggg
    16921 gtaattctgg cgagcagcag caccaatacc ggattgctgg cgcgtgaatt tatctgcaaa
    16981 ggggaggaag cctgatgagc cagtcactgt ttagccaacc attgaacgtt attaacgtcg
    17041 gcatcgccat gtttagcgat gacctgaaaa agcagcatgt agaagtgact caactcgact
    17101 ggacgccgcc ggggcagggc aatatgcagg tggtgcaggc gctggacaac attgccgatt
    17161 cgccgctggc agacaaaatc gccgccgcta accagcaggc gctggagcgc attatccagt
    17221 cgcatccggt gctgattggt tttgagcagg cgattaacgt ggtgccgggc atgacgccga
    17281 aaaccattct tcacgccggg ccgccgatca cctgggaaaa aatgtgcggc gcgatgaaag
    17341 gcgcggtcac cggagcgctg gtgttcgaag gactggcgaa agatctcgac gaggcggctg
    17401 aactggcggc ttccggggag atcactttct cgccgtgtca cgagcacgac tgcgtgggat
    17461 cgatggcggg tgttacctcg gcctcgatgt ttatgcacat cgtaaaaaac aaaacctacg
    17521 gcaacatcgc ttataccaac atgagcgagc agatggcgaa gattttgcgt atgggcgcta
    17581 acgaccagag cgtgattgac cgcctgaact ggatgcgtga tgtgcaggga ccaatactgc
    17641 gcgacgcgat gaaaattatc ggcgaaatcg atctgcgctt aatgctggcg caggcgctgc
    17701 atatgggcga tgagtgccat aaccgcaata acgccgggac gacgctgctg attcaggcgc
    17761 tgacgccggg gattatccag gcgggctatt ccgtcgcgca acagcgcgaa gtgtttgagt
    17821 ttgttgccag tagcgattac ttctccggcc cgacgtggat ggcgatgtgt aaagcggcga
    17881 tggatgcggc gcatggcatt gaatacagca ccgtggtcac caccatggcg cgtaatggcg
    17941 tcgagttcgg cctgcgggtc agcggcctgc cggggcagtg gtttaccggc ccggcgcagc
    18001 aggtcatcgg cccgatgttt gccggttata agccggaaga ttcagggctg gatattggcg
    18061 acagcgccat cacggaaacc tacggtattg gcggatttgc catggcgacc gcgcctgcaa
    18121 tcgtcgcgct ggtgggtggc acggtggaag aggctatcga tttctcccgt cagatgcgcg
    18181 aaatcaccct tggcgaaaac cccaacgtca ccattccgct gctcggtttt atgggcgtgc
    18241 cgtcggcaat cgacatcacc cgcgtgggta gcagcggcat tctgccagtg atcaacaccg
    18301 ccatcgccca taaagatgcg ggcgtcggca tgattggtgc gggcattgtg catccacctt
    18361 ttgcctgctt cgagaaagcc attcttggct ggtgcgaacg ttacggcgtc tgacctacat
    18421 gttcatgccg gatgcggcgt gaacgcctta tccggcctac gaatggcgcg agaatctgta
    18481 ggcctgataa gcgtagcgca tcaggcattt gtcaccattg ccggatgcgg cgtgaacgcc
    18541 ttatccggcc tacgagtggc gcgagaattt gtaggcctga taagcgtagc gcatcaggca
    18601 tttgtcacca ttgccggatg cggcgtgaac gccttatccg gcctacgaat ggcacgaaaa
    18661 tttgtaggcc tgataagcgt agcgcatcag gcagtctggc gttggtcata accccatcac
    18721 cctctgtagc ggacataaca acatgaaaga gcttgtggtc gttgccattg gtggcaacag
    18781 cattatcaaa gataacgcca gccagtcgat tgagcatcag gcggaggcgg tgaaagccgt
    18841 cgccgatacg gtgctggaaa tgctggcttc cgattacgac attgtgctga cccacggtaa
    18901 cgggccgcag gtcgggctgg atttacgccg tgcggagatt gcccacgagc gcgaagggct
    18961 gccattaacg ccgctggcga actgtgtggc agatacgcag ggcggcatcg gctatctgat
    19021 ccaacaggcg ctgaataacc ggctggcgcg tcacggcgag aaaaaagccg tcaccgtggt
    19081 gactgaggtg gaagtggata aaaacgatcc gggatttgct catcccacca agcccatcgg
    19141 cgcattcttc agtgaaagcc agcgtgacga attacaaaag gcaaaccctg actggtgttt
    19201 tgttgaagat gccggacggg gctatcgccg cgtggtcgcc tcgccggaac cgaaacgtat
    19261 tgtcgaagca cccgccatta aggcgctgat ccaacaaggc tttgtggtga ttggcgcggg
    19321 cggcggcgga attcctgtgg tgcgtactga cgcgggagat taccaaagcg tggacgcggt
    19381 tatcgacaaa gatctctcta ccgtgctgct ggcccgtgaa attcacgccg acattctggt
    19441 gatcaccacc ggcgtggaaa aagtgtgtat tcattttggc aaacccgagc agcaggcgct
    19501 cgatcgggtg gatattgcca ccatgactcg ctatatgcag gaaggacatt tcccgcccgg
    19561 cagcatgttg ccaaaaatca tcgccagcct gacgttcctg gaacaaggcg gcaaagaagt
    19621 gattatcacc acgccggaaa gcctgcccgc ggcgctgcgt ggtgagacgg gcactcatat
    19681 tattaaaacg taaggacgta agatgaaaga aagcaatagc cgccgtgagt ttctgagcca
    19741 gagcggtaag atggtcaccg ccgccgcgct gtttggtacc tctgtgccgc tcgcccatgc
    19801 ggcggtagct ggcaccctaa actgcgacgc gaataacacc atgaaaatca ctgacccgca
    19861 ttactatctc gacaacgtgc tgctggaaac tggatttgac tacgaaaatg gcgtggcggt
    19921 gcagacccgc acggcgcgcc agaccgtgga gattcagaac ggcaaaattg tcgcgctgcg
    19981 cgagaataag cagcacccgg atgccacgct gccgcactat gacgctggcg ataagctgat
    20041 gctgcccacc acccgcgaca tgcatattca tctcgacaaa accttttacg gcgggccgtg
    20101 gcgctcgctc aatcgcccgg caggcaccac catccaggac atgatcaaac tcgagcagaa
    20161 aatgctgccg gagctgcaac cgtacacgca ggaacgggct gaaaaactga tcgatttatt
    20221 acagtcgaaa ggcaccacca ttgcccgcag ccactgcaat atcgaaccgg tttccggcct
    20281 gaaaaatctg caaaatttgc aggcggtgct ggcgcgacgt caggcgggct ttgagtgtga
    20341 aatcgtcgcc ttcccgcagc acggtttgct gctgtcgaaa tcggaaccct taatgcgtga
    20401 agcaatgcag gcgggtgcgc attacgtcgg cgggctggac ccgaccagtg tcgatggcgc
    20461 gatggaaaaa tccctcgata ccatgttcca gattgcgctg gactacgaca aaggcgtcga
    20521 tattcacctg cacgaaacca ctccgtcggg cgtggcagcc atcaattata tggttgaaac
    20581 ggtagagaaa acgccgcaac tgaagggcaa gctgaccatc agccacgcct ttgcgctggc
    20641 tacgctcaac gaacaacagg tcgatgaact ggcgaatcgg atggcggcgc agcaaatttc
    20701 tatcgcctcg acggtgccga ttggcacgct gcatatgccg ctcaaacagt tgcacgacaa
    20761 aggcgtaaaa gtgatgactg gcactgacag cgttatcgac cactggtcgc cttatggtct
    20821 gggcgacatg ctggaaaaag ccaatctcta cgcgcagctc tatattcgtc ctaacgaaca
    20881 gaacctctcc cgctcgctgt ttttagccac tggtgatgta ttgccgctca acgaaaaagg
    20941 cgagcgtgta tggccaaaag cgcaggatga cgccagcttt gtgctggtgg acgcctcctg
    21001 ttccgccgag gcggtggcgc gtatctcgcc gagaaccgca acgttccata aagggcaact
    21061 ggtgtggggg agtgtggcag gttgatgcgc atttgaaacg cctgcaacgg tgaatagtaa
    21121 gagatttaag ccccagggag tggggcaatc tgaatatgag tgtgtcggca gaaatggaca
    21181 ttatctttga ggaatatgcc cttattgacg ttgtgtagat aactatttgt ctacgtgaaa
    21241 agatcatcag ttttgccgat tttagtcttt tacggatagc aaatatcaca cttatagacg
    21301 ctcgccttat ggggaacgtt gctgctctgt ttcattcagg ctcgcggcat gttggggctg
    21361 gatcgggtgg tttaaatatt ggctatattc aatggacgcg ttttgccgcg atgacatatc
    21421 aggcgtcgcc aaatacacat agctaatcag gagtaaacac aatgaagatc aaagctgttg
    21481 gtgcatattc cgctaaacaa ccacttgaac cgatggatat cacccggcgt gaaccgggac
    21541 cgaatgatgt caaaatcgaa atcgcttact gtggcgtctg ccattccgat ctccaccagg
    21601 tccgttccga gtgggcgggg acggtttatc cctgcgtgcc gggtcatgaa attgtggggc
    21661 gtgtggtagc cgttggtgat caggtagaaa aacatgcgcc gggcgatctg gtcggtgtcg
    21721 gctgcattgt cgacagttgt aaacattgcg aagagtgtga agacgggctg gaaaactact
    21781 gtgatcacat gaccggcacc tataactcgc cgacgccgga cgaaccgggc catacgctgg
    21841 gcggctactc acaacagatc gtcgttcatg agcgatatgt tctgcgtatt cgtcacccgc
    21901 aagagcaact ggcagcggta gccccattac tgtgcgcggg gatcactact tactccccgt
    21961 tgcgccactg gcaggccgga ccgggtaaaa aagtgggcgt ggtcggcatc ggcggtctgg
    22021 gacatatggg gatcaagctg gcccacgcga tgggggcaca tgtggtggca tttaccactt
    22081 ctgaggcaaa acgcgaagcg gcaaaagccc tgggggccga tgaagttgtt aactcacgca
    22141 atgccgatga gatggcggct catctgaaga gtttcgattt cattttgaat acagtagctg
    22201 cgccacataa tctcgacgat tttaccacct tgctgaagcg tgatggtacc atgacgctgg
    22261 ttggtgcgcc tgcgacaccg cataaatcgc cggaagtttt caacctgatc atgaaacgcc
    22321 gtgcgatagc cggttctatg attggcggca ttccagaaac tcaggagatg ctcgattttt
    22381 gcgccgaaca tggcatcgtg gctgatatag agatgattcg ggccgatcaa attaatgaag
    22441 cctatgagcg aatgctgcgt ggtgatgtga aatatcgttt tgttatcgat aatcgcacac
    22501 taacagactg aaaaaattaa taaataccct gtggtttaac atattaactt cgctctccac
    22561 ttaacttttt agttaaggag agcgaaataa tatcaaagta gcagtaaaac ctataacgta
    22621 aattcaaatt gttaaattaa ctccctccag tacacaatac ttcacacgtt agttatgagc
    22681 gatttctgat agtgcctggt ttaatcagag ctttattatc tgcgacgttt atttttattt
    22741 aagagagatc gcgatgatat cattaaaagc tccgcacaat aatttgatgc catatacgca
    22801 acaaagcata cttaatacgg ttaaaaacaa tcagttacca gaggatatca aaagctccct
    22861 ggtttcctgt gtcgatatat tcaaggtttt gattaaacaa tattatgatt atccttatga
    22921 ttgtcgtgat gatttagtcg atgacgacaa actcattcat ctcatggctg ccgtacgaga
    22981 ctgtgagtgg tcagacgata acgcactcac cataaatgtg cagtttaatg atttccctgg
    23041 attttatgac tggatggatt accctgatca cccggttaag tttgtttttc gcatacttga
    23101 aaatcaaaaa gggacagttt gggtatatga ccaggatgat gcgtttcttg atattaaagc
    23161 aaacgtgcag gctggacgct ttaccgggct taagaaatta gtgcagttta tcgattcagt
    23221 gcgaacagat tgcaaatgta ttcttcttga acaccatatg cctttgctga gacttttccc
    23281 taaagggaaa gagtgtatgc atgtggaaaa atggcttcgc gaaatgtcgt ctattccgga
    23341 gacagacgcg cccattaagc aggtgcttgc gcatggatta ttattacact taaaaaatat
    23401 ctaccctgtt tttcctgaat cactggtgat gttattgctc tctgttctgg acgtaaaaac
    23461 atacagagat gacgcacgat taaatgaatg gatatctaat agagtgcagg aattaggtga
    23521 cagatattat cctgtaaata agcacgttaa aatccgctac actctttagt tcagaaaggg
    23581 ttccacctgc aagttgttat tccacctgag agtgaatcgc aatgcaggta atcatttcat
    23641 ctgtatatct gtatgccaac tggcattcca tctctttttt cgccgatatt ttgttgtgat
    23701 cggcgacact tcgcttaaaa aagcaccagt agtggtttcg cagccatgcg gtgtataaaa
    23761 aaaatgatct catgcagatg ttttgtgaat gtgtgggttg acattcatat gaaaaaaatc
    23821 ataattccat catgtttgtg tggggtcttt tctgtatctt acgcatcgca ctcaagcctg
    23881 acagaaaata tgctgtaagg ctcatatcaa aaaccgccat tagctcatca ggaagagcag
    23941 acgacaacca ggattgttgt atggtacggg gttcgaggcc tcgatggcgg tccaactttt
    24001 caaaactctt ctgaatcaga tccccttatt atcatccctg caaagtggaa cattgccatt
    24061 gtttggctat tcaggacggg tcagaccaat gaaaaaagcc cacacagggg agagtgggct
    24121 gaaatgggaa gctaaagact caagtaaact tatcggaaat aaggaccacg cattacgggg
    24181 gctatcatcg ccgatggcct ggattcggca aatcaggttg attacgtaga gagtaaatta
    24241 tctgctcacc gctgcgtcac cccttcgtaa atcaggcgta gcgcgaatac accaataatt
    24301 gcaccaataa cccgactggc aacgcgttgc atacgcccat aagcacgacg cacagcgggc
    24361 aaagaaaacg cctgactaag aaagactcgc cagataactg atgcgagcac aatccccgcc
    24421 caggccatta aacgtgccca tgttggtgtt tcggcattta atgttactga gaaaatactg
    24481 ataaaaaata aaacggtttg cgggttagag agatcggtaa ttaatccgcg gcgaaaaaag
    24541 acataccagg gggcgctaat cggttgttgt agtgtgctca tttgcggtgt tgactggcgg
    24601 cgcatgctgc accacgcaaa ccataagaga taagcgccgc cgacgattct gataagcgaa
    24661 aaaatctcct cacactgcgt aattagtgtt gcaagaccaa acaaacccaa cccggaataa
    24721 aatgcatcgc ccagcgccac gcccagcccg gtcagcaccc ctgcgcgtcg accggaagcc
    24781 aggctggttt gtactaccac aaagagattg gctcccggat taaaaaaagt aatcacgaac
    24841 agtcccacgg tcaggtaaac ggcatgcaaa ggatccatag tgatttcatc cataaataag
    24901 tgaactaact gcatcatatt tctaccaaaa ataatcgggt gcgagagaga tcacaaagtg
    24961 tcttatttcc ggttactggc gtttatgccc tgactgaact aattattaat caacccaata
    25021 atgtgggtgg gtgatagtgt gataacaact ctggagccgt aatatgaaaa taatctctaa
    25081 aatgttagtc ggtgcgttag cgtttgccgt taccaatgtc tatgccgctg aattgatgac
    25141 caaagcggaa tttgaaaaag ttgaatcgca gtatgaaaaa ataggtgata tttcaaccag
    25201 taatgaaatg tcgactgcag atgcaaaaga agatttaatc aaaaaagccg atgaaaaagg
    25261 ggctgatgtg ttggtgctga cctctggtca aactgacaat aagatccacg gcacggcgga
    25321 tatttataag aagaagtaat tctgaaccct atgtaaacat ccccgatgcg taagtttatc
    25381 ggtgatcatc atctattgaa atttatgccg gataaagcgt tcgcgctgca ttcggcagtt
    25441 cagcttttca gccgccgcca gaacgtcgtc cgactgatgc ctaaataatt cgccgctgct
    25501 gttttatcgc cattaaattt ctccagtgcc tgttgtggtg tcagtaagcg tggagcggga
    25561 gttttcgccg actcgcgcgc cagttccggc agtagcagct gcaaaaattg cggcgttaaa
    25621 tccggcgtcg gttccacact taaaaatagc gccagtcgct ccatcatatt gcgcagttca
    25681 cgaatattgc ccggccagtc gtagcgcagc agcacagttt cacttgcctg taacccctgg
    25741 cgtaatgcag cagaaaacgg ggcggagagc gccgccagag acactttcaa aaaactttcc
    25801 gccagtggca gaatatctgc cacccgctcg cgcagtggtg gcaattgcag acgcaaaata
    25861 ctcaaccgat aaaacagatc acggcgaaac tgcccttgcc gcatatcttc ttccagattg
    25921 cagtgagtgg cgctaatgac ccgcacatcc accggaacag gctgatgccc gccgacgcga
    25981 gtaacctctt tttcttccag cacccgtaac aggcgagtct gcaaaggtag cggcatttcg
    26041 ccaatctcat ccaaaaacag cgtgccaccg tgggcgattt cgaacagtcc ggcgcgacct
    26101 ccgcgtcgcg agccggtaaa cgccccttcc tcatagccaa acagctccgc ttccagcagc
    26161 gattcggcaa tcgccccgca gttgacggca acaaacggat gcgacttttt gccctgtcgc
    26221 gcatcgtggc gggcaaaata ttcccgatga attgcctggg ccgccagctc tttgcccgtc
    26281 cccgtttccc cctcaatcaa caccgccgca ctggagcggg catacagcaa aatagtctgc
    26341 cgtacttgtt ccatctgtgg tgattgaccg agcatatcgc ccagcacgta acgagtacgc
    26401 agggcgttac gggtagcatc gtgagtgtta tggcgtaacg acatgcgcgt catatccagc
    26461 gcatcgctga acgcctggcg cacggtggcg gcggaataga taaaaattcc ggtcattccg
    26521 gcttcttctg ccagatcggt aatcagcccc gcgccgacca ccgcttcggt gccgttagct
    26581 tttagctcgt taatctgccc gcgtgcgtct tcttcggtaa tgtagctacg ttgatcgagg
    26641 cgcaaattaa aggttttttg aaacgccacc agtgccggaa tagtttcctg ataagtgaca
    26701 acgccgatag aagaggtgag ctttccggct tttgccagtg cttgtaacac atcgtagccg
    26761 ctcggtttaa tcaaaataac tggcactgac aggcggcttt tcaggtacgc gccgttagag
    26821 ccagcagcga tgatggcgtc acagcgttcg tttgccagtt tcttgcggat gtaggtcact
    26881 gctttttcaa agccaagctg aataggggta atgttcgcca ggtgatcaaa ctcgaggctg
    26941 atatcgcgaa acagctcgaa caggcgcgtt acagataccg tccagataac cggtttgtcg
    27001 tcattcagcc gtggtggatg tgccatagcg caccgcaaag ttaagaaacc gaatattggg
    27061 tttagccttg tttcataatt gttgcaatga aacgcggtga aacattgcct gaaacgttaa
    27121 ctgaaacgca tatttgcgga ttagttcatg actttatctc taacaaattg aaattaaaga
    27181 tttaatttta ttaaggcaat tgtggcacac cccttgcttt gtctttatca acgcaaataa
    27241 caagttgata acaaaggatg ggctatgtct ctacactctc caggtaaagc gtttcgcgct
    27301 gcactgtcta aagaaaatcc attgcagatt gttggcacca ttaacgccaa ccatgcgctg
    27361 ttggcgcagc gtgccggata tcaggcaatt tatctctccg gcggtggcgt ggcggcaggg
    27421 tcgctggggc tgcccgatct cggtatttct acccttgatg atgtgctgac cgacattcgc
    27481 cgtatcaccg acgtttgttc gctgccgctg ctggtggatg cggatatcgg ttttggttct
    27541 tcggccttta acgtggcgcg tacggtgaaa tcgatgatta aagccggtgc ggcaggattg
    27601 catattgaag atcaggttgg tgcgaaacgc tgcggtcatc gtccgaataa agcgatcgtc
    27661 tcgaaagagg agatggtgga tcggatccgc gcggcggtgg atgcgaaaac cgatcctgat
    27721 tttgtgatca tggcgcgcac cgatgcgctg gcggtagagg ggctggatgc ggcgatcgag
    27781 cgtgcgcagg cctatgttga agcgggtgcc gagatgttgt tcccggaggc gattaccgaa
    27841 ctcgccatgt accgccagtt tgccgatgcg gtgcaggtgc caatcctcgc caacattacc
    27901 gaatttggcg ccacgccgct gtttaccacc gacgaattac gcagcgctca tgtcgcgatg
    27961 gcgctgtacc cgctttcagc gtttcgcgcc atgaaccgcg ccgccgaaca tgtctacaac
    28021 gtcctgcgcc aggaaggcac gcagaaaagc gtcatcgaca ccatgcagac ccgcaacgag
    28081 ctgtacgaaa gcatcaacta ctaccagtac gaagagaagc tcgacgacct gtttgcccgt
    28141 aaccaggtga aataataaac gcccgttggt tgtattcgac aaccgatgcc tgatgcgccg
    28201 ctgacgcgac ttatcaggcc tacgggtgta ctgaactgta ggtcggataa gacgcatggc
    28261 gtcgcatccg acaacaatct cgaccctaca aatgataaca atgacgagga caacatgagc
    28321 gacacaacga tcctgcaaaa cagtacccat gtcattaaac cgaaaaaatc agtggcgctt
    28381 tctggcgttc cggcgggcaa tacggcgctc tgcaccgtgg gtaaaagtgg caatgacttg
    28441 cattaccgtg gttacgatat tcttgatctg gcggaacatt gcgaatttga agaagtggcg
    28501 catctgctga tccacggcaa actgccgacc cgtgacgaac tcgccgccta caaaacaaaa
    28561 ttgaaagccc tgcgcggttt accggctaac gtgcgtactg tgctggaagc cttaccagcg
    28621 gcgtcgcatc cgatggatgt catgcgcacc ggcgtttctg cgatcggctg cacgctgcca
    28681 gaaaaagagg ggcacaccgt ttccggtgcg cgggatattg ccgacaaact gctggcatca
    28741 cttagctcga ttctccttta ttggtatcac tacagccata acggcgaacg catccagccg
    28801 gaaaccgatg acgactccat cggcggtcac ttcctgcatc tgctgcacgg cgaaaagccg
    28861 tcgcaaagct gggaaaaggc gatgcatatt tcgctggtgc tgtacgccga acacgagttt
    28921 aacgcctcca cctttaccag tcgggtgatt gcgggcactg gctctgatat gtattccgcg
    28981 attattggcg cgattggcgc actgcgcggg ccgaaacacg gcggggcgaa tgaagtgtcg
    29041 ctggagatcc agcaacgcta cgaaacgcca gacgaagccg aagccgatat ccgcaagcgg
    29101 gtggaaaaca aagaagtggt cattggtttt gggcatccgg tttataccat cgccgacccg
    29161 cgtcatcagg tgattaaacg tgtggccaag cagctctcgc aggaaggcgg ctcgctgaag
    29221 atgtacaaca tcgccgatcg cctggaaacg gtgatgtggg agagcaaaaa gatgttcccc
    29281 aatctcgact ggttctccgc tgtttcctac aacatgatgg gcgttcccac cgagatgttc
    29341 acaccactgt ttgttatcgc tcgcgtcacc ggctgggcgg cgcacattat cgaacaacgt
    29401 caggacaaca aaattatccg tccttccgcc aattatgttg gaccggaaga ccgccagttt
    29461 gtcgcgctgg ataagcgcca gtaaacctct acgaataaca ataaggaaac gtacccaatg
    29521 tcagctcaaa tcaacaacat ccgcccggaa tttgatcgtg aaatcgttga tatcgtcgat
    29581 tacgtcatga actacgaaat cagctctaaa gtggcctacg acaccgcaca ttactgcctg
    29641 ctcgacacgc tcggctgcgg tctggaagct ctcgaatacc cagcctgtaa aaaactgctg
    29701 gggccaattg tccccggcac cgtcgtacct aacggcgtgc gcgtccccgg aactcagttc
    29761 cagctcgacc ccgtccaggc ggcatttaac atcggcgcga tgatccgttg gctcgatttc
    29821 aacgatacct ggctggcggc ggagtggggg catccttccg acaacctcgg cggcattctg
    29881 gcaacggcgg actggctttc gcgcaacgcg gtcgccagcg gcaaagcgcc gttgaccatg
    29941 aaacaggtgc tgaccggaat gattaaagcc catgaaattc agggctgcat cgcgctggaa
    30001 aactccttta accgcgtcgg cctcgaccac gttctgttag tgaaagtggc ttccaccgcc
    30061 gtggtcgccg aaatgcttgg cctgacccgc gaggaaattc tcaacgctgt ttcgctggcg
    30121 tgggtggacg gtcagtcgct gcgcacctat cgccatgcgc cgaacaccgg cacgcgtaaa
    30181 tcctgggcgg cgggcgatgc cacttcccgc gcggtacgtc tggcactgat ggcgaaaacg
    30241 ggcgaaatgg gttacccgtc agccctgact gcgccggtat ggggcttcta cgacgtctcc
    30301 tttaaaggtg aatcgttccg cttccagcgt ccgtacggtt cttacgtcat ggagaatgtg
    30361 ctgttcaaaa tctccttccc ggcggagttc cactcccaga cggcagttga agcggcgatg
    30421 acgctctatg aacagatgca ggcagcaggc aaaacggcgg cggatatcga aaaagtgacc
    30481 attcgcaccc acgaagcctg tattcgcatc atcgacaaaa aagggccgct caataacccg
    30541 gctgaccgcg accactgcat tcaatacatg gtggcgatcc cgctgttgtt cgggcgctta
    30601 acggcggcag attacgagga caacgttgcg caagataaac gcatcgacgc cctgcgcgag
    30661 aagatcaatt gctttgaaga tccgacgttt accgctgact accacgaccc ggaaaaacgc
    30721 gccatcgcca atgccgttac tcttgagttc actgacggta cgcgctttga agaagtggtg
    30781 gtggagtacc ccattggtca tgctcgccgc cgtcaggatg gcattccgaa gctggtagat
    30841 aaattcaaaa tcaatctcgc gcgccagttc ccgactcgcc agcagcagcg cattctggag
    30901 gtttctctcg acagagctcg cctggaacag atgccggtca atgagtatct cgacctgtac
    30961 gtcatttaag taaacggcgg taaggcgtaa gttcaacagg agagcattat gtcttttagc
    31021 gaattttatc agcgttcgat taacgaaccg gagcagttct gggccgagca ggcccggcgt
    31081 attgactggc agacgccctt tacgcaaacg ctcgatcaca gcaatccgcc gtttgcccgt
    31141 tggttttgtg aaggccgaac caacttgtgt cacaacgcta tcgaccgctg gctggagaaa
    31201 cagccagagg cgctggcatt gattgccgtc tcttcggaaa cagaagaaga gcgcaccttt
    31261 acctttcgcc agctgcatga cgaagtgaac gcggtggcgt caatgctgcg ctcactgggc
    31321 gtgcagcgtg gcgatcgggt gctggtgtat atgccgatga ttgccgaagc gcatattacc
    31381 ctgctggcct gcgcgcgcat tggcgctatt cactcggtgg tgtttggggg attcgcctcg
    31441 cacagtgtgg cggcgcgaat tgatgacgct aaaccggtgc tgattgtctc ggcagatgcc
    31501 ggagcgcgcg gtggcaaaat cattccctat aaaaaattgc tcgacgatgc gataagtcag
    31561 gcgcagcatc agccgcgcca tgttttgctg gtagatcgtg ggctggcgaa aatggcgcgc
    31621 gttagcgggc gggatgtcga tttcgcgtcg ttgcgccatc agcacatcgg cgcgcgggtg
    31681 ccggtggcct ggctggaatc aaacgaaacc tcctgcattc tctacacctc cggcacgacc
    31741 ggcaaaccta aaggcgtgca gcgtgacgtc ggcggatatg cggtggcgct ggcgacctcg
    31801 atggacacca tttttggcgg caaagcggga ggtgtgttct tttgtgcttc ggatatcggc
    31861 tgggtggtgg ggcattcgta tatcgtctac gcgccgctgc tggcggggat ggcgactatc
    31921 gtttacgaag gattaccgac ctggccggac tgcggcgtgt ggtggaaaat cgtcgagaaa
    31981 tatcaggtta gccggatgtt ctcagcgccg accgccattc gcgtgctgaa aaaattccct
    32041 accgctgaaa ttcgcaaaca cgatctctcg tcgctggaag tgctctatct ggctggagaa
    32101 ccgctggacg agccgaccgc cagttgggtg agcaatacgc tggatgtgcc ggtcatcgac
    32161 aactactggc agaccgaatc cggctggccg attatggcga ttgctcgcgg tctggacgac
    32221 aggccgacgc gtctgggaag ccccggcgtg ccgatgtatg gctataacgt gcagttgctt
    32281 aatgaagtca ccggcgaacc gtgtggcgtc aacgagaaag ggatgctggt ggtggaaggg
    32341 ccgctgccgc cggggtgtat tcagaccatc tggggcgacg acgaccgctt tgtgaagacg
    32401 tactggtcgc tgttttcccg cccggtgtac gccacctttg actggggcat ccgcgatgct
    32461 gacggttatc actttattct cgggcgcact gacgatgtaa ttaacgttgc cgggcatcgg
    32521 ctggggacgc gcgagattga agagagtatc tccagtcatc cgggcgttgc cgaagtggcg
    32581 gtggttgggg tgaaagatgc gctgaaaggg caggtggcgg tggcgtttgt cattccgaaa
    32641 gagagcgaca gtctggaaga tcgtgatgtg gcgcattcgc aagagaaggc gattatggcg
    32701 ctggtggaca gccagattgg caactttggc cgcccggcgc acgtctggtt tgtctcgcaa
    32761 ttgccaaaaa cgcgatccgg aaaaatgctg cgccgcacga tccaggcgat ttgcgaagga
    32821 cgcgatccgg gggatctgac gaccattgat gatccggcgt cgttggatca gatccgccag
    32881 gcgatggaag agtaaggtat tgtcggatgc gtcgcgtggc gcatccgaca gttgtgcgca
    32941 gatgcctgat gcgacgctgg
//
LOCUS       DAZFKF010000049        32922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFKF010000049 DAZFKF010000000
VERSION     DAZFKF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.049"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..144
                     /locus_tag="ACHKA1_002764"
     CDS             <1..144
                     /locus_tag="ACHKA1_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5680390.1"
                     /translation="ALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRVGW
                     AQE"
     gene            complement(584..913)
                     /locus_tag="ACHKA1_002765"
     CDS             complement(584..913)
                     /locus_tag="ACHKA1_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO5680391.1"
                     /translation="MNNPNPCMTCGACCAFFRVSFYWAEADDAGGKVPVSLTEQISPF
                     HRCMRGTSQKNPRCVALAGTPGKNAYCSVYKNRPSTCREFAMSGENGIANEACNRARA
                     KYGLPEI"
     gene            complement(1054..1141)
                     /locus_tag="ACHKA1_002766"
     tRNA            complement(1054..1141)
                     /locus_tag="ACHKA1_002766"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1105..1107),aa:Ser,seq:tga)
     gene            1567..2685
                     /gene="hyaA"
                     /locus_tag="ACHKA1_002767"
     CDS             1567..2685
                     /gene="hyaA"
                     /locus_tag="ACHKA1_002767"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO5680392.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2682..4475
                     /gene="hyaB"
                     /locus_tag="ACHKA1_002768"
     CDS             2682..4475
                     /gene="hyaB"
                     /locus_tag="ACHKA1_002768"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO5680393.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            4494..5201
                     /gene="hyaC"
                     /locus_tag="ACHKA1_002769"
     CDS             4494..5201
                     /gene="hyaC"
                     /locus_tag="ACHKA1_002769"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO5680394.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            5198..5785
                     /gene="hyaD"
                     /locus_tag="ACHKA1_002770"
     CDS             5198..5785
                     /gene="hyaD"
                     /locus_tag="ACHKA1_002770"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO5680395.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLGAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            5782..6180
                     /gene="hyaE"
                     /locus_tag="ACHKA1_002771"
     CDS             5782..6180
                     /gene="hyaE"
                     /locus_tag="ACHKA1_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO5680396.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            6177..7034
                     /gene="hyaF"
                     /locus_tag="ACHKA1_002772"
     CDS             6177..7034
                     /gene="hyaF"
                     /locus_tag="ACHKA1_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO5680397.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            7168..8712
                     /gene="appC"
                     /locus_tag="ACHKA1_002773"
     CDS             7168..8712
                     /gene="appC"
                     /locus_tag="ACHKA1_002773"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO5680398.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQSFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            8724..9860
                     /gene="appB"
                     /locus_tag="ACHKA1_002774"
     CDS             8724..9860
                     /gene="appB"
                     /locus_tag="ACHKA1_002774"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO5680399.1"
                     /translation="MFDYETLRFIWWLLIGVILVAFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            9873..9965
                     /gene="cbdX"
                     /locus_tag="ACHKA1_002775"
     CDS             9873..9965
                     /gene="cbdX"
                     /locus_tag="ACHKA1_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO5680400.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            10045..11349
                     /gene="appA"
                     /locus_tag="ACHKA1_002776"
     CDS             10045..11349
                     /gene="appA"
                     /locus_tag="ACHKA1_002776"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO5680401.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPEPELKLESVVIVSRHGVR
                     APTKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCP
                     QSGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLD
                     NANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQAL
                     PSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNA
                     QFYLLQRTPEVARSRATPLLDLIMTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANL
                     GGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSL
                     NTPPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(11469..13649)
                     /gene="etk"
                     /locus_tag="ACHKA1_002777"
     CDS             complement(11469..13649)
                     /gene="etk"
                     /locus_tag="ACHKA1_002777"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO5680402.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(13669..14115)
                     /gene="etp"
                     /locus_tag="ACHKA1_002778"
                     /gene_synonym="yccY"
     CDS             complement(13669..14115)
                     /gene="etp"
                     /locus_tag="ACHKA1_002778"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO5680403.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(14103..15242)
                     /gene="gfcE"
                     /locus_tag="ACHKA1_002779"
     CDS             complement(14103..15242)
                     /gene="gfcE"
                     /locus_tag="ACHKA1_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO5680404.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(15288..17384)
                     /gene="gfcD"
                     /locus_tag="ACHKA1_002780"
     CDS             complement(15288..17384)
                     /gene="gfcD"
                     /locus_tag="ACHKA1_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO5680405.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(17384..18130)
                     /gene="gfcC"
                     /locus_tag="ACHKA1_002781"
     CDS             complement(17384..18130)
                     /gene="gfcC"
                     /locus_tag="ACHKA1_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO5680406.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLAVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVIVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(18127..18771)
                     /gene="gfcB"
                     /locus_tag="ACHKA1_002782"
     CDS             complement(18127..18771)
                     /gene="gfcB"
                     /locus_tag="ACHKA1_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO5680407.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(18878..19183)
                     /locus_tag="ACHKA1_002783"
     CDS             complement(18878..19183)
                     /locus_tag="ACHKA1_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680408.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(19625..19837)
                     /gene="cspH"
                     /locus_tag="ACHKA1_002784"
     CDS             complement(19625..19837)
                     /gene="cspH"
                     /locus_tag="ACHKA1_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO5680409.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            20123..20335
                     /gene="cspG"
                     /locus_tag="ACHKA1_002785"
     CDS             20123..20335
                     /gene="cspG"
                     /locus_tag="ACHKA1_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO5680410.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            20346..20534
                     /gene="ymcF"
                     /locus_tag="ACHKA1_002786"
     CDS             20346..20534
                     /gene="ymcF"
                     /locus_tag="ACHKA1_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO5680411.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            20509..20739
                     /gene="ymcE"
                     /locus_tag="ACHKA1_002787"
     CDS             20509..20739
                     /gene="ymcE"
                     /locus_tag="ACHKA1_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO5680412.1"
                     /translation="MHRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            20729..20902
                     /gene="gnsA"
                     /locus_tag="ACHKA1_002788"
     CDS             20729..20902
                     /gene="gnsA"
                     /locus_tag="ACHKA1_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO5680413.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(20950..22023)
                     /gene="yccM"
                     /locus_tag="ACHKA1_002789"
     CDS             complement(20950..22023)
                     /gene="yccM"
                     /locus_tag="ACHKA1_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO5680414.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRYMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(22095..24839)
                     /gene="torS"
                     /locus_tag="ACHKA1_002790"
     CDS             complement(22095..24839)
                     /gene="torS"
                     /locus_tag="ACHKA1_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO5680415.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFIQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTLEVGSCFCLRLPLRVATAPAPKTANQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLEHYL
                     QLQVNNDIPLDVSQLNEDAQLMGTEKIHEWLALFKQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            24922..25950
                     /gene="torT"
                     /locus_tag="ACHKA1_002791"
     CDS             24922..25950
                     /gene="torT"
                     /locus_tag="ACHKA1_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO5680416.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQSSTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(25923..26615)
                     /gene="torR"
                     /locus_tag="ACHKA1_002792"
     CDS             complement(25923..26615)
                     /gene="torR"
                     /locus_tag="ACHKA1_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO5680417.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQVQPLTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            26745..27917
                     /gene="torC"
                     /locus_tag="ACHKA1_002793"
     CDS             26745..27917
                     /gene="torC"
                     /locus_tag="ACHKA1_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO5680418.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRAGANDSGDKLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            27917..30463
                     /gene="torA"
                     /locus_tag="ACHKA1_002794"
     CDS             27917..30463
                     /gene="torA"
                     /locus_tag="ACHKA1_002794"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO5680419.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSKNLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTIAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVDQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            30460..31059
                     /gene="torD"
                     /locus_tag="ACHKA1_002795"
     CDS             30460..31059
                     /gene="torD"
                     /locus_tag="ACHKA1_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO5680420.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAMDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(31152..31457)
                     /gene="cbpM"
                     /locus_tag="ACHKA1_002796"
     CDS             complement(31152..31457)
                     /gene="cbpM"
                     /locus_tag="ACHKA1_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO5680421.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(31457..32377)
                     /gene="cbpA"
                     /locus_tag="ACHKA1_002797"
     CDS             complement(31457..32377)
                     /gene="cbpA"
                     /locus_tag="ACHKA1_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO5680422.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            32637..>32922
                     /locus_tag="ACHKA1_002798"
     CDS             32637..>32922
                     /locus_tag="ACHKA1_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680423.1"
                     /translation="MGNIHGISCTANNYLKQAWNDIKNEYEKNKTYSITLFENTLVCF
                     MRLYKEIRRQQAEDYIPCLECDSLEKEFEEIQNDNDLSLFMKTLRTNDKQI"
BASE COUNT         7866 a         8369 c         8666 g         8021 t
ORIGIN      
        1 gcgctgagtg aagcggcaga gcaggcgatg ttcaccaaag ggatggaaat ccacgtccgg
       61 cagcgcacca tgaagaaaga gctggaaaag ctgaccagtg cggatgagat actggcatac
      121 agggttggct gggcacagga ataacagaca aaaagatggg ggaccatcac ctcccccatg
      181 aactgagtcg attatgtaat tattgtgaag agaccttacc tacacagata aagagtgtca
      241 cggaaaagtt aatcctgaaa gtgatgcagg tctcatttcc agagcatcct gttgatatgg
      301 ataatttttc agatttccgg aagtgagagt ttttggtgtt tccataccag ccacaattga
      361 tgtgttttat ggcgcctggc agtgcgatta agggaacagg gggtaataaa aaaaacgggg
      421 gtatcaccac acccccggta caagaaagga gtaactataa tggaaaaatt aatgaagacc
      481 tttagtgcta cgttatatgg caaataaaga ctgccacagg gaagtttgca gaaacagtga
      541 tgccgatcgt gttcctgtgc tcttctctga taagagtaat ttttcagatt tccggaagtc
      601 cgtattttgc ccgggcgcgg ttgcatgctt cattcgcgat gccattttct ccggacattg
      661 cgaattcccg gcatgtggac ggacggtttt tgtaaacaga gcaatatgcg tttttacccg
      721 gagttcccgc cagagcgaca catcggggat ttttctgact ggtgccgcgc atacagcgat
      781 gaaaggggga tatttgctca gtgagactga ccggaacctt tccgccagca tcgtcagctt
      841 ctgcccagta aaaagagacg cggaaaaatg cacaacaggc accacacgtc atgcatggat
      901 tggggttatt cataattcat cggtatagcg ataaatcgta aagagaacaa tgtacctagt
      961 gtgtattgag ctaaatatac caattagtat accaagtgta tactaagagc aaaaaaagga
     1021 aaggggctac catttggtaa ccccttgatt gtttggcgga agcgcagaga ttcgaactct
     1081 ggaacccttt cgggtcgccg gttttcaaga ccggtgcctt caaccgctcg gccacacttc
     1141 cggaatgacg cgcactataa acatcccgat gcggcgtgta aacccctaat ttgtttgttt
     1201 gcctgaaaaa cagccaaaag tgcattgata gcgtgaaata aaagcagatt gatcatttca
     1261 tcaccataaa ttccttctct tttactcgtt tagcaactgg ctaaacatcc caccgcccgg
     1321 caaaaagaaa aataggtcca tttttatcgc taaaagataa atccacacag tttgtattgt
     1381 tttgtgcaaa agtttcacta cgctttatta acaatacttt ctggcgacgt gcgccagtgc
     1441 agaaggatga gctttcgttt tcagcatctc acgtgaagcg atggtttgcc ttgctacagg
     1501 gacgtcgctt gccgaccata agcgcccggt gtcctgccgg tgtcgcaagg agaagagacg
     1561 tgcgatatga ataacgagga aacattttac caggccatgc ggcgtcaggg cgttacccgg
     1621 cgcagctttc tcaaatattg tagtctggct gccacgtcgc tgggattagg cgcgggaatg
     1681 gcaccaaaga ttgcctgggc gctggagaac aaaccgcgca ttccggtagt atggatccac
     1741 ggtctggaat gcacctgctg taccgaatct tttatccgct ccgctcaccc gctggcaaag
     1801 gacgtcatcc tttccctgat ttccctcgat tacgacgata ctttgatggc tgccgccgga
     1861 acccaggcgg aagaagtatt tgaagacatc atcacgcaat acaatggcaa atatatcctc
     1921 gcagtagaag gtaatccgcc gctgggcgag caggggatgt tctgtatcag cagcggtcga
     1981 ccgtttattg agaaactcaa acgtgccgct gccggagcca gcgcgattat cgcctgggga
     2041 acctgcgcgt cctggggctg cgtgcaggcc gcgcgaccta atccgacaca ggcaacgcct
     2101 atcgacaaag tcatcaccga caaacccatt atcaaagtac ctggctgccc gccgatcccg
     2161 gatgtgatga gcgccatcat tacttacatg gtgacctttg atcgcttgcc agatgtcgac
     2221 agaatgggtc gtccactgat gttttatggt cagcgtatcc acgataaatg ctatcgccgc
     2281 gctcacttcg acgccggaga gttcgtccag agttgggatg atgacgctgc ccgcaaaggt
     2341 tattgcctgt acaaaatggg ctgcaaaggg cctaccacct ataacgcctg ttcctccaca
     2401 cgctggaatg atggcgtttc tttcccaatc cagtctggtc acggctgcct gggctgtgcg
     2461 gaaaatggtt tctgggatcg cggttcgttc tacagccgcg tggtcgatat tccgcaaatg
     2521 ggtactcatt ccaccgccga taccgtcggt ttaaccgcgc ttggcgtggt ggcagcggct
     2581 gttggtgtgc acgcagtcgc cagcgccgtt gaccagcgca gacgtcataa ccagcaacct
     2641 acagaaaccg aacatcagcc aggcaatgag gataaacagg catgagcact cagtacgaaa
     2701 ctcagggata caccatcaat aatgccggac gccgcctggt ggtcgacccg attacgcgca
     2761 ttgaaggcca catgcgctgc gaagtgaata ttaacgatca gaatgtgatc accaatgccg
     2821 tctcctgcgg caccatgttt cgcgggctgg agatcatcct gcaagggcgc gacccgcgcg
     2881 atgcgtgggc gttcgttgaa cgtatctgcg gcgtctgtac tggcgtacac gccctggctt
     2941 cggtttacgc catcgaagat gcaatcggta ttaaagtgcc ggacaacgcc aatatcatcc
     3001 gcaacattat gctggcaacg ctctggtgcc acgatcatct ggtgcacttc tatcagctcg
     3061 ccgggatgga ctggatcgat gtgttagatg cgctgaaagc cgacccgcgg aaaacctccg
     3121 aactggcgca aagtctctcc tcatggccga aatcatcccc tggctatttc ttcgacgtac
     3181 aaaatcgcct gaagaaattc gttgaaggcg ggcagttggg gatcttccgc aatggctact
     3241 gggggcaccc gcagtacaaa ctgccgccag aagccaacct gatgggcttt gcccactatc
     3301 tcgaagctct cgatttccag cgtgaaattg tcaaaatcca cgcggtcttt ggcggtaaaa
     3361 acccgcatcc aaactggatt gtcggcggga tgccatgcgc cattaacatt gacgaaagcg
     3421 gcgcggtcgg ggcggtcaat atggaacgcc tgaacctggt gcagtcgatt atcacccgca
     3481 cggcagactt cattaacaac gtgatgatcc ccgacgcctt agccatcggt cagttcaaca
     3541 aaccgtggag cgaaatcggc acggggcttt ctgataaatg tgtcctcagc tacggcgcat
     3601 tcccggatat tgccaacgac tttggtgaga aaagtctgct gatgcctggc ggcgcggtga
     3661 ttaacggcga cttcaacaat gtgctgccag tggatttggt tgatccgcag caggtgcagg
     3721 agtttgtcga tcacgcctgg tatcgttatc ccaacgatca ggtcgggcgt catccattcg
     3781 atggcattac cgacccgtgg tacaaccccg gcgatgtcaa aggcagcgat accaacattc
     3841 agcagctgaa tgaacaggaa cgctactcgt ggatcaaagc gccgcgctgg cgcggtaacg
     3901 cgatggaagt ggggccgctg gcacgcacgt taatcgctta tcacaaaggc gatgctgcga
     3961 ccgttgagtc ggtcgatcgc atgatgtcgg cgctgaacct gccgctttcc ggtatccagt
     4021 caacgttggg ccgcattttg tgccgcgcgc acgaagcaca gtgggccgca ggtaagttgc
     4081 agtatttctt caacaagctg atgactaacc tgaaaaacgg caatcttgcc accgcctcca
     4141 cggaaaaatg ggaacccaca acctggccga cagagtgccg tggtgtcggc tttaccgaag
     4201 cgccgcgcgg ggcgttaggc cactgggccg ccattcgcga tggcaagatt gatctctacc
     4261 agtgcgtggt gccgaccacc tggaacgcca gcccgcgcga tcccaaaggg cagattggcg
     4321 cttatgaagc ggcgctgatg aacaccaaaa tggcgatccc cgagcaaccg ctggagatcc
     4381 tgcgtactct gcacagcttt gatccgtgcc tcgcctgttc aacacacgtg ctgggcgacg
     4441 acggtagcga gctgatctcc gtgcaggtgc gttaacagcg aaggagaatc atcatgcaac
     4501 agaaaagcga caacgttgtc agccactatg tctttgaagc gccagtgcgt atctggcact
     4561 ggctgacggt gttatgcatg gcggtgttga tggtcaccgg ttactttatc ggcaaaccgc
     4621 taccttccgt cagcggcgag gcgacgtatc tgttctatat gggctacatc aggttaattc
     4681 acttcagcgc cgggatggtt tttaccgtgg tcttgctgat gcggatctac tgggcttttg
     4741 ttggcaatcg atactcccgc gagctgttta tcgtgccagt atggcgtaaa agctggtggc
     4801 agggcgtgtg gtatgaaatc cgctggtatc tgtttctggc aaaacgtccg agtgccgata
     4861 taggccataa ccccatcgcc caggcggcga tgtttggcta tttcctgatg tcggtcttta
     4921 tgatcatcac cggctttgcg ctgtacagcg aacacagcca gtacgctatt tttgcgccgt
     4981 tccgttatgt ggtggaattt ttctactgga cgggaggcaa ctcaatggac attcacagct
     5041 ggcatcggct ggggatgtgg ctgattggcg cgtttgtgat cggtcatgtc tatatggcgc
     5101 tgcgtgaaga catcatgtcc gacgacacgg tgatctccac catggtcaac ggctaccgta
     5161 gccacaaatt tggcaaaata agtaacaagg agcgttcatg agcgagcaac gcgtggtggt
     5221 catggggctg ggcaacctgc tgtgggccga tgaaggtttc ggcgtgcggg tggcggaacg
     5281 gttgtatgcc cattaccact ggcccgagta tgtggagatt gtcgatggcg gtactcaggg
     5341 actgaacttg ctggggtatg tcgaaagcgc cagccatctg ttgattctcg atgccattga
     5401 ctacgggctg gaacctggaa cgctgcgaac ctatgccgga gaacgtattc cggcttatct
     5461 cagcgcgaag aaaatgagcc tgcaccagaa cagtttctcc gaagtgctgg cgctggcaga
     5521 tatccgcgga catctgccag cacatattgc cctcgtcggt ctgcaacccg caatgctcga
     5581 cgactacggc ggcagcctga gcgaactggc acgggagcaa ctgcccgcag cggaacaggc
     5641 ggcgctggcg cagctgggtg catggggaat tgtgccgcaa ccggctaatg aatcgcgctg
     5701 tctcaattat gactgtctgt cgatggaaaa ttacgaaggc gttcgcttgc gccagtacag
     5761 gatgacactg gaggagcagg gatgagcaac gacacgccat ttgatgcgtt gtggcaacga
     5821 atgctggcgc gcggctggac gccggttagt gaattccgtc ttgacgactg gcttacgcaa
     5881 gcgccagacg gcgtggtgtt attaagcagt gacccgaaac gcacgccaga ggtcagcgat
     5941 aatccggtaa tgattggcga attactgcgc gagtttcccg actacacatg gcaggtggcg
     6001 attgctgacc ttgaacagag cgaagccatc ggagatcgct ttggcgtctt tcgctttccc
     6061 gccacattag tgtttaccgg cggaaactat cgcggcgtgc tgaatggtat tcacccgtgg
     6121 gcggaactga taaacctgat gcgtgggctt gtcgaaccac aacaggagcg tgcctcatga
     6181 gcgaaacttt tttccatctg ctggggccag gaacgcaacc gaacgatgac agtttcagca
     6241 tgaatccgct gccgatcacc tgtcaggtga atgatgaacc gagtatggcg gccctggaac
     6301 agtgtgctca cagcccgcag gtgattgcgc tgttaaacga gttacaacat caactaagcg
     6361 aacgccagcc gccgttgggc gaggtgctgg cagtcgatct gttaaatctc aatgccgacg
     6421 atcgtcactt tatcaatacg cttctcgggg aaggggaagt gtcagtgcgc attcagcagg
     6481 ctgacgacag cgaaagtgaa atacaggagg cgatcttctg cggattatgg cgggtgcgca
     6541 gacgtcgcgg cgaaaagttg ctggaggaca aactggaagc tggctgcgcg ccgctggcgt
     6601 tgtggcaggc ggcaacgcaa aatctcttgc cgacagattc gctgttaccg ccgcccattg
     6661 atggcctgat gaatggccta ccgttggcgc atgagttact ggcgcatgta cgtaaccccg
     6721 acgcgcagcc gcacagcatt aatctgacgc aattacccat cagcgaggct gatcggcttt
     6781 ttctctcacg tctctgtggg ccgggaaata ttcagattcg taccattggc tatggcgaga
     6841 gctatatcaa cgccacgggg ttacgccatg tctggcattt acgctgtacg gacaccttaa
     6901 aaggcccgtt actggaaagt tatgaaatct gcccaatacc ggaagtggtg ctggcagcgc
     6961 cagaagattt ggtcgactct gcgcagcggc ttagcgaggt atgtcagtgg ctggcggaag
     7021 gtgcaccgac gtaaaaagac ggtaagtatc gctttcagtc ttatgaatat cgcaatcggc
     7081 gaatacctct ggtcgtagag tttcaggata aagagggaga tctaccatta tcgggttatt
     7141 tttctctctt cgcctacagg agtgcgcatg tgggatgtca ttgatttatc gcgctggcag
     7201 tttgctctga ccgcgctgta tcacttttta tttgtacccc ttaccctggg gctgattttt
     7261 ttgctggcta tcatggaaac catttacgtg gtcaccggca aaacaatcta ccgcgatatg
     7321 acgcgcttct ggggtaagct cttcggtatc aactttgctc ttggcgtggc taccggcctg
     7381 accatggagt ttcagtttgg tactaactgg tcattctatt ccaactatgt gggcgatatt
     7441 tttggcgcac cgctggcgat ggaagcatta atggccttct tcctcgaatc cacctttgtc
     7501 gggcttttct tcttcggctg gcagcggctg aataaatacc agcacctgct ggtgacgtgg
     7561 ctggtggcgt tcggttcaaa tctctctgca ttgtggatat tgaatgccaa cggttggatg
     7621 caatacccga ccggtgcgca ttttgatatc gacaccctgc gtatggagat gaccagcttc
     7681 agcgagctgg tctttaaccc ggtcagccag gtgaaatttg tgcacaccgt aatggcgggc
     7741 tacgtgaccg gggccatgtt tattatggcg atcagcgcct ggtatttact gcgcggacgg
     7801 gagcgcgatg tcgcattacg ctcgtttgcc atcggttccg tcttcggtac tctggcgatt
     7861 attggcacgc tgcaactcgg agacagttct gcgtatgaag tcgcgcaagt acaaccggta
     7921 aaactggcgg cgatggaagg ggagtggcaa acggaacctg cacctgcacc gttccatgtg
     7981 gttgcatggc cggaacagga tcaagagcgt aacgcctttg ccatcaaaat tcccgcgctg
     8041 ctagggatcc tcgccactca ctcattagat aaacccgtgc cgggtctgaa gaatttgatg
     8101 gctgaaacct acccacgctt gcaacgcgga cgtatggcct ggctgttaat gcaggaaata
     8161 tcgcaaggca atcgtgagcc gcatgtgttg cagtcattcc gggagctgga aggcgacctg
     8221 ggctacggca tgttgctctc ccgctatgcg ccggatatga atcatgtcac agccgcacag
     8281 taccaggcgg cgatgcgtgg cgcgatacct caggttgcgc cggtattctg gagtttccgc
     8341 atcatggtgg gctgtggttc cctgctgcta ctggtgatgc ttattgcgct tgtccagacg
     8401 ctgcgtggca aaatcgacca gcatcgctgg gtgctgaaaa tggcgctctg gagtttgccg
     8461 ctgccgtgga ttgcgattga agccgggtgg tttatgaccg agtttggtcg gcagccgtgg
     8521 gcgatacagg acatcttacc gacatactcc gcgcactccg ctttaaccac aggacaactg
     8581 gctttctcac tgatcatgat cgtaggcctt tacaccctgt tcttaatcgc cgaagtctac
     8641 ctgatgcaga aatatgcccg tctggggccg agcgcgatgc agagtgaaca accgacgcag
     8701 caacaggggt aaaggagaaa atcatgtttg attatgaaac attgcgcttc atctggtggc
     8761 tgctgattgg cgtgatcctg gtggccttta tgatctccga cggatttgac atggggattg
     8821 gctgtttgct gccgctggtg gcgcgtgatg atgatgaacg ccggatagtg attaatagcg
     8881 ttggtgcgca ctgggaaggc aatcaggtct ggttgatcct cgcaggtggg gcattatttg
     8941 ccgcctggcc cagagtgtat gcagcggcgt tttccggctt ttatgtggcg atgatcctgg
     9001 tgctgtgctc gctgttcttc cgcccgctgg cctttgatta tcgcggaaaa atcgccgatg
     9061 cacgctggcg taaaatgtgg gatgccggtc tggtcatcgg cagtctggtg ccgccggtag
     9121 tcttcggtat cgccttcggc aacttgttgc tcggcgtgcc gtttgccttc actccgcaat
     9181 tacgcgtgga gtatctcggc agcttctggc aactgctgac gccattccct ttattgtgcg
     9241 gattgctcag ccttgggatg gtgattttgc aaggtggcgt ctggttacaa ctgaaaactg
     9301 ttggtgtgat tcatctgcgt tcacagctgg cgactaaacg cgctgcactg ttggtgatgc
     9361 tgtgcttttt gctggcgggt tactggctgt gggtcggtat tgatggcttt gtactgctcg
     9421 cccaggatgc taacggtcct tccaatccgt taatgaaact ggtggcagtg ctacctggtg
     9481 cctggatgaa taattttgtc gagtcgcccg ttttgtggat cttcccgctg ctggggttct
     9541 tctgcccatt gctgacggtg atggcgattt atcgtggtcg cccgggttgg ggatttttga
     9601 tggcgtcatt gatgcaattt ggcgtgattt tcacggcagg catcacgctg ttcccctttg
     9661 tcatgccgtc aagcgtgagt ccaatctcca gcctgacgtt gtgggacagt acttccagtc
     9721 agctgacgct gagcattatg ttggtaatcg tgctgatatt tttgcccatt gtgttgctct
     9781 acactctctg gagctactac aaaatgtggg gacgcatgac gacagaaact ctccgccgta
     9841 acgaaaacga gctgtactaa ggagcaggaa caatgtggta tttactttgg ttcgtcggca
     9901 ttttgttgat gtgttcgctc tccacccttg tgttggtatg gctggacccg cgattgaaaa
     9961 gttaacgaac gtaagtctga tgcggcgcat tagcgtcgta tcaggcaatc aataatgtcg
    10021 gatatgaaaa gcggaaacat atcgatgaaa gcgatcttga tcccattttt atctcttctg
    10081 attccgttaa ccccgcaatc tgcattcgct cagagtgagc cggagccgga gctgaagctg
    10141 gaaagtgtgg tgattgtcag tcgtcatggt gtgcgtgccc caaccaaggc cacgcaactg
    10201 atgcaggatg tcaccccaga cgcatggcca acctggccgg taaaactggg ttggctgaca
    10261 ccgcgcggtg gtgagctaat cgcctatctc ggacattacc aacgccagcg tctggtagcc
    10321 gacggattgc tggcgaaaaa gggctgcccg cagtctggtc aggtcgcgat tattgctgat
    10381 gtcgacgagc gtacccgtaa aacaggcgaa gccttcgccg ccgggctggc acctgactgt
    10441 gcaataaccg tacataccca ggcagatacg tccagtcccg atccgttatt taatcctcta
    10501 aaaactggcg tttgccaact ggataacgcg aacgtgactg acgcgatcct cagcagggca
    10561 ggagggtcaa ttgctgactt taccgggcat cggcaaacgg cgtttcgcga actggaacgg
    10621 gtgcttaatt tcccgcaatc aaacttgtgc cttaatcgtg agaaacagga cgaaagctgt
    10681 tcattaacgc aggcattacc atcggaactc aaggtgagcg ccgacaatgt ctcattaacc
    10741 ggtgcggtaa gcctcgcatc aatgctgacg gaaatatttc tcctgcaaca agcacaggga
    10801 atgccggagc cggggtgggg aaggatcact gattcacacc agtggaacac gttgctaagt
    10861 ttgcataacg cgcaatttta tttactacaa cgcacgccag aggttgcccg cagccgcgcc
    10921 accccgttgt tggatttgat catgacagcg ttgacgcccc atccaccgca aaaacaggcg
    10981 tatggtgtga cattacccac ttcagtgctg tttatcgccg gacacgatac taatctggca
    11041 aatctcggcg gcgcactgga gctcaactgg acgcttccag gtcagccgga taacacgccg
    11101 ccaggtggtg aactggtgtt tgaacgctgg cgtcggctaa gcgataacag ccagtggatt
    11161 caggtttcgc tggtcttcca gactttacag cagatgcgtg ataaaacgcc gctgtcatta
    11221 aatacgccgc ccggagaggt gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag
    11281 ggcatgtgtt cgttggccgg ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc
    11341 agtttgtaat gtataaaaaa gagcattcag ctacctgaat gctctgaggc tgatgacaaa
    11401 cgaagaactg tctaatgcgt agaccggaaa aggcgttcac gccgcatccg gtcactttac
    11461 cttcagtttt actctttctc ggagtaacta taaccgtaat agttatagcc gtaactgtaa
    11521 gcggtgctgg cgcgtttaat cacaccattg aggatagcgc ctttaatatt gacgcctgcc
    11581 tgttccagac gctgcattga caaactcacc tctttggcgg tgttcaggcc aaaacgtgca
    11641 accagcaggc tggtgccgac agagcgcccc acgaccgcag catcgctcac cgccagcatc
    11701 ggcggcgtat cgacaatcac cagatcgtaa tggtcgttcg cccattccag taattgacgc
    11761 atccgatcgc gcatcagcag ttcagacggg ttaggtggca cctgaccgcg agtaatcaca
    11821 tcaaagcctc ctttgccaaa atgctggatc actttgttga gctcatcttt acctgccaga
    11881 tattccgaca agccatgttc attactcacg gtaaacaggt tatgcgaata accacggcgt
    11941 aagtcggcat caataaataa cactttttga tcggactggg cgatcaccgc tgccagagtt
    12001 gaactgacaa atgttttacc actgtctggc gtcgcaccgg tgatcatcag aatgttattc
    12061 tcagtctcca tcatagcgaa atgcagactg gttcgtagcg cacgtacggc ttccacagca
    12121 gaatccgccg ggttatccac cgccaggaag gggatatttt tagtacgatg gcgctgctga
    12181 ttagaaaata aatttttctt acgcagacgg gtgcgtttat ccagccactc ggacattggg
    12241 atagtggcat aaacgctgat gccgtgctct tccagttgtt ccggtgcttc tacaccacga
    12301 cgcaacatcg cacgcgccag cacggcaccc accgaaataa acaggccaag aataaaacca
    12361 agcaccacgt tcaacgcttt tttcggtttc actggctgcg gctgagtgac tgccgggtcg
    12421 ataatccgca cgttaccaat ggcactggat ttcgaaatac tcaactcctg ctggcggtta
    12481 agtaattgca gatataccgc acggcccgct tctacgtcac gacttaaacg caacacttcc
    12541 tgttgggtgg aaggcatagc cgatacccgc ttattcaggc gtttgcgttc ttgctccagc
    12601 gtctggcgtt tttccagcag cgcacgataa gttgggtgat ctttcttata cagctgggag
    12661 atctctgcct cgcggaaggt cagctcattg agttgattat caacgttcac aatctgctca
    12721 agaacggctt tggcttccag gttaaggtca accgaatcgc gctgctggcg ataaacgttg
    12781 agtttttctt ccgcctggtc cagctcgctg cgcacttcag gcaactggcg ctgtaagaat
    12841 tcaaggcttt gtgaatcctg cgccgcctgg cgagcgatat tctgttgcaa atagttgtta
    12901 gcgatgctgt tcagaatacg ggtaatcaac tggggatcat caccggtcat ggtaagttcc
    12961 agcatcccgc tttctttact gcgttcgcta acggtaaagg tttcctgcaa cgcgttaatc
    13021 gcttccagtt cggtacgttg gctcaggaca aactgtgttc ctggtttggc cttaatatcc
    13081 gctatagtca gcgcaacgcc atctttttcc agacgctggc cgaccatacc attgacggtg
    13141 aactcttcac cttccagcgt atagtggccg ttttccccaa ccgtgagtgt cagttgctga
    13201 tcctgaccat tcagttgtgg aatatgcatc cagctgatcg ccagctcacc tggtttctct
    13261 ttggttaatc tcgcccagcc gcgacccaca atcggaaaat acttctgctc aaccatgtcg
    13321 cgcagattca gttcagcaat ggttttaccg agaatcatgc gcgattgcag cagttggatc
    13381 tccggtgcag actcgggcga tgagttaggg atcatatcac tcaggccgct gagaatggcg
    13441 ttgccctgtt tttgctcaac ctggaccaga gtatctgcct gataaattgg tgtgcttaac
    13501 agcgagtaag cgacagcgat cagcgtgaat aacgcggtca cgctgataat aaacttacgg
    13561 tgatcccata actcgccgac cagacgaagc agatcgatct cattttcctg agtgctgcct
    13621 ggtggcgtat tcatattttt agttgtcatc gtattttata aactcaaatt accggctgag
    13681 gcgcttcgcc cattcctgac tggcgcgctc caacataccg tagacatgtt caaatgcgtc
    13741 ctgactttta cgatagggat ccgggatctc tttctgttcc agccattgcc caaacagcat
    13801 tgtcttcccg cggacctcgg gagcgattgc ggtaacctga gcaatatgtt ccgactccat
    13861 tgccagaatc agatcgtaat ttcgtgccat ctcagcagtg agcttacgtc cggcatgtcc
    13921 ttcaagagat acgccatgat tagcggcaac atctgccgct gtcgcatctg cagggtgttt
    13981 taccagacca tgaacacctg ccgatttgac ttttacgccc ggcagacgtt tacgcagcaa
    14041 gcgctcgcca atgggcgaac ggcaaatgtt gccggtacaa accaccagga ttgagttaaa
    14101 ttttagttgg gccatgtttg aatccaccgt gaagtctctg tcaggtcatg caccccggaa
    14161 atcgtcggta ccagcagaga aatcacacgg ttccaacgag ccagaggcgc ggtagtgaca
    14221 taaacaatgt cgtaaggttc aagctggaat tccgttccga gtatcattgc cgatgcgtcc
    14281 ttcgcattca gctgataaat attggcgatt ttgccattct gcttgttttg cgttgcacga
    14341 ataacaaaaa tccccgtcgc atcagcgaca tcctgattca taccctcagc gttacccagc
    14401 gcttccgcca gggtcatacc gctgcggtcc attttcagcg tgctctgttt acccacttca
    14461 cccatcacga agactttcag atcatcgttg cgcggaataa ataaaatatc tcccgggtgc
    14521 agcagtttgt tctggcgcaa gtcgccacgc tgcatcagcg catagaggtt cactttggtc
    14581 tttacgccat tctgggtgag caccacattg cgccagtctg catctgcggt cagaccgcct
    14641 gcggcgttaa tcgcatccat gatggtcaga ggaatattgg taatcggctg ctgacccgat
    14701 ttgctgactt cgccggtgac gtacgctttt tgcgatcgga acgccgcgac gctgacatcg
    14761 acctgcgggc tttcaatgac tgaatccaga cgcgctgtaa tctcgttgcg tacctgggta
    14821 agtgttttcc cggccacttt caggcgacca atataggggt agaaaatcgc accatcggcg
    14881 ttaacccagt tgccggtatc actggcgcta cggtattgcc ctgcgggggt ggttagttcc
    14941 gggtgatccc agaccgtaac catcaacaca tcaccaatgc cgatacgata ttcatagttc
    15001 gcgatttgct gctccagctc cgggttagcc tgcgacataa tcggtttggc gcgcaactga
    15061 tcaatcaatc ctggcgtgac gggatagata ttgaccattt tgtccaggtc atactgattg
    15121 tcaggtagtt caatgacatc cttgttactg gtcgataaat tctgaccagg aacaagggta
    15181 catgctgtca aagacagaac agccaatgtc agtaccgaaa acttaaaaat gttctttttc
    15241 attggtttat atttatcagt caatgtaatg ttatgaatta cgtcaggtta ttgtccaacc
    15301 ggtacttctc gctctgccgt cattggatag agctggtatt tgcgtgacag catttgtcca
    15361 ccatcacgcg tcaatggtgt ccacgaaacc accgcgcggt tgcggttagg tccaatggtc
    15421 atcaagtcga acggaatcga gatataaaaa cctttactaa agccgccttc gccgtagtca
    15481 tctttagata cgttactgat tgccgcccat acccctaccg ccacgccgct gtcaaagcgt
    15541 ttggcgacgt cgatcgttgc gcctttatct tttgccagat attgcccaac gctaagtttc
    15601 atcagtacgc cattgagcgt cggcgggttc cagtaagccg tcacgaaacc tgtgggcgtg
    15661 gaataatcgg tgaagcgcat catgttgtcc cagtcacgtt gcttcacgta gttaacgtcc
    15721 acacccagcg cccagctggc atctagcggg cgataaagca gctcggaacc gacaccggcg
    15781 tacatcgttt ccagataacc gccatacacc tggccataga aaccattgcc taagtcggca
    15841 aagtagttcg cctgcaagtt gttgagataa acgtcattgc gaacgtagtc acggatatgc
    15901 gtgcgcacgc ggggcagggt agagtccgcg ggcaacagtg aagacttaaa cttgtcgtag
    15961 ttgttgtaaa tattggtgaa aataccgcca tcaagcagca ggtggtcggt aaaccagtag
    16021 cgggcgctgg acatcagccc cagctggaac atatagaaat cttccgggcc gcccagcgac
    16081 tgtgaaagtg ttgggttgaa agagtagcta aagcgatctt cacgaatacg gtagccccga
    16141 ccaaaggcag aaagatcttc agcttcaaca cgttgttgtt gcagtggctc tgattgacca
    16201 ggcgctgtac ctgccagctg tttgcgcagg ctggctacgt cggtttcggt agtcaccatc
    16261 gccatatgct cgcgcttttg cgtcacgcta atcttctcaa cgccttgcgg caggttattc
    16321 accagaatcc ggttggcacg atcgaccgct tcacgagagt cacggtattt gtattgctga
    16381 ccagacatat acagcgtctt atcgcgtagc tgaatttctg gcgcgtcaaa gcccgcgtta
    16441 tacttcaggg cggtaagttg gtttgctacc gtggtgtact gcaatccttc agattcaggc
    16501 gcaggttgat atgccggttt tggcgtatcg cgcagggcag gacgcagatc gttgaaattg
    16561 gtccgtaaag tgaaaccaaa catcaacgtg ttaccgcgtt cataactcag gttgaggtct
    16621 gcccagctgg cagcgcgata aactgcaccg acgttgaaat ggcttgcctg aggcagtttg
    16681 ccagcgaaat cattctggta attgttgccg tcgtattcga gtttcagacg caggggattc
    16741 cacggcgttt gatactcaat gccgccaaag atggaagccg ggccacgaaa gatatcgcta
    16801 aagctgatat cgcccgcatc gtgagactct gcgcgatgac aatatttatc gctcacccgg
    16861 caaaacgggt tggtaatatt gcccgcatta ccggcgtagc cccatgccat cccgagggtg
    16921 aaatcaaatg gccccgcttg cttgctggcc accaaatact caccgtcaaa cagacccgta
    16981 ccagcaatat cacgtttacc aaacgccact tgcggtagcc agtaaccttc ttcccacagg
    17041 cgaagtttaa aatcgaatga tttgtctttg tatgactgat cgccgctgaa atcttcccac
    17101 tggctatatt tacgtgtgcg cacatccgta taacgaatgg tgccttccag ccatgggaac
    17161 agcgccacgg aggtggagta gaaacggtat tgatcgttat cccggtagtt gacgctgaat
    17221 tcaccttccg gtgcgatgcg cgcatttggc atctgcaaca atcctgtgcc gccaaagtcc
    17281 gattgcgacg aacccagcgg atccgggtag gttaatactt cagcatgaca ggcactggag
    17341 acggcaatgg ccaggcagct taaaagataa gaattcttct tcatcagtca ggaacacgtt
    17401 gcgttaaaac agacacaatc tggtcattta aatcagcata tttttcgggc agtacatgcg
    17461 cagaaaagcc taaccaaagc tggctgccag gcggtggttc cacatgtctt ttattccaca
    17521 gggcaacagg ggcgacgaca gtctcaccct caggcgtaat cacgataacg ttattcttgt
    17581 ccgcgcctgc aagacggggg tgatcttgca gataatcggt aacgctacgg cctgcctgcc
    17641 agggcaattg cccagcccca gaaaccgcac ccagcagagt aatagtcacc ggacgctgta
    17701 cggtatagag cgtatagtcc ccgaccagcg ggggattgct gttctcatcc actcgtacaa
    17761 aatcagggtc cagtttgacc gctaagcgac cagtgatatt gagattgagt aactgctggc
    17821 gcacggattt aatcgttgcc gccacatcgt cgtcagcttc tgcttcccag gaagcaagct
    17881 gtgccatgac atgttggtag tctttaagcg ctttagcttt cgccgcagag tcggtcagca
    17941 aggcaccagg ccaccagagg cgatcgcgca gttgcggttg tgtcaccaac tgggcaacat
    18001 tttccacggg gccaacggaa agtgtctgtt gttcgccagg cagataaata gtcaccgttc
    18061 cttgcgcaaa ggcatggggt gtcattacgt aaagtacgct ggcaatgaaa tacgactgta
    18121 atttattcat ggtgcgggtt taagaaacgt catttctacg ggaataaccc ctgcgcctaa
    18181 catttgccga ctttgacgaa cctggccagt agccgaatcg acccagaatg tgttttgcca
    18241 ctgtttttct ggcagcgtgg aagtcacctc ttcctgccag acattacaca gcacatcgcg
    18301 acccgcaata tgcagcgtct ctaatccagc aaaggaaaaa gtggaactca gggtcgcaga
    18361 gcgaaaatgg ttgtcttcac tccagagaat atcgcgcgtc cagcggctgc cgtcttttat
    18421 tgccagcgcg ttacgcagag ggtcctgccc ggaatttgtc acttccagca gattattatt
    18481 aagtttgacg gtctttaaaa gacgtccatt gtgggtaacc agcatggcgt tatcctggga
    18541 taaccatttg ctttgttctt gttctatata tcccagtacc acaaagattc gttgcccttc
    18601 attaaggcgt aaatacatcg tggaataagg caacgcctgg atctgctgat ccgctacggt
    18661 gatatcctga ttatcgaaaa ggctggcgcg aaatgtatcg accatacttt gctggctgtg
    18721 cgtacatccc gcaagaaata gtgcgaaaat cgataaaata agagggcgca cctttaatcc
    18781 ttgaaaggtt aattaaaaaa atgcaaatct ctaaagcccg gtacgaatat cgcaaccggg
    18841 tattatttaa ctttaatttc gacgcgttta agcagaatta gcgcgtagtg gacgtggtgg
    18901 ttgaggtgtt atgagaaccg ccatcgccac tgtcatttgc tgcaactact gccaacgtcg
    18961 ccacaccaac agccgttgcc acaccgacaa ctgtcgccgt gccgacagcg ccagcagcca
    19021 cgccaccagt ggtagcagcc gtcgtggttg caccggtagt ggtcgttgtg gtgccagtag
    19081 tacccgtcgt tgcctcggtc ccgtttgctg cttcaggggc agcaaacgca gctggcgtcg
    19141 tcaacatgaa ggccataagg attgcagaaa gcttgtgttt cataactttt cctttattca
    19201 tcgcatggac aatacgtacg cttgagatca ggattgtcta ctttgagtat aacatccttg
    19261 tgaaatctcc ctttcctcat accacttgca ctatatacag cgggtgagaa aggcgcgcag
    19321 agggaaatat aagattgtta actattcccc tctggaaata cgacttgtga gcataagata
    19381 aatcttagca aaatacaaat taaagataag aaaattgtta agaaagctat taactattat
    19441 tggcaatata gtttcaagtg gtactataac tcgctgttaa gtggcattta taactattag
    19501 ttgtagagat attcggttaa atggattagt tttccggtga aaaagtaact tgtgttataa
    19561 tacatggtgt tgactattta actatgccaa taaaaccagt cagctttaaa caagcagcgt
    19621 catattaaga gagataaaca tttgccgctg ttggtcctcg caggccattt acgcggcaaa
    19681 attccacgcg taatcctggt ataagcactt ctgcgtcgcg gggagtgaat gcggaaatat
    19741 ggacctgaac ttctttacga ccgtcggagg ggataatgaa tcctttgccg cttttgcgat
    19801 caaaggtttt gacaattcct gtcattttac gggacaaaca aattccttac tgaaaatact
    19861 gcgctgcact atacggggtt aataaaataa agccagcgat atttaagacc gccggacggc
    19921 taaaataaaa tttgcttaat ctcaattatc atgcgttaat agctgcgtcg gtttgaaaga
    19981 cagacagcat acaaagtagt ttactaaagc agttctcatt atcaggcatt atccccttct
    20041 tttgagtctc tctcctgaac actaagtagt ttctgtatta aagccctgtt tgccgaaagg
    20101 cccaaaatga aggaagtaaa atatgtctaa taaaatgact ggtttagtaa aatggtttaa
    20161 cgcagataaa ggttttggct ttatcactcc tgatgatggc agcaaagacg ttttcgtcca
    20221 tttcaccgcc atccagagca atgaattccg cacgctgaac gaaaatcaga aagttgaatt
    20281 ttctattgag caggggcaac gtggccccgc ggcagcgaac gtagttacgc tctaaggttg
    20341 ccgttattac tcaacatctc cattttcgct gtccatgttg tcatggttca cagtaccgca
    20401 catcggcatt cgatgtgacg gagcgaaacc ctttgggcgc taagtgtatt ttttgtaaat
    20461 cgacgatgat cacctttgat aacgtcgcgc tacaaatacg cactgaccat gcaccgctgg
    20521 atttcacaaa ataatatcag gctccctcgt ggagcctttt ttatatctgc cttatttttc
    20581 ttcaacgctg tatgtatagt aagcgataac ctgttgatta ttgaatcttt cggggagatg
    20641 gcttataaca tttcttacct gaccagggta ccgggaacca acaccttact ggcgtgttgc
    20701 tgtctttcaa gaccagacga ggttaacagt gaatattgaa gagttaaaaa aacaagccga
    20761 aacggaaatc gccgacttta tcgcgcaaaa aatcgccgag ctgaacaaga atacagggaa
    20821 agaagtctct gaaattcgct tcaccgcacg agaaaaaatg accgggcttg aaagttatga
    20881 tgtcaaaatc aaaataatgt gattttgtga acatcacccg tgcgaggtga tgttccgctt
    20941 gttgctaatt tagtgaccaa tcattggcgc ttgcggaatt aagcgtcggt acaattcctc
    21001 cggcaccggg ctttgccata ctcccgcata cattgcgtaa ccaatcaccg caaacataat
    21061 ccccagaacc agtagcgtca ttaaccagcc agacaacgca aaggcttttt tatttgccgc
    21121 aggtttttgc agtgaaaagg tcaatgttga ggctaccgga catgactcta cgcaagtcat
    21181 acagccggta cattccactg ttcgtacctg aattaattta tcgaccggga tccgtgatgg
    21241 gcaatttttt gcgcatttgc cacagtcgat acaactttcg gcattgcgac gaatcttaaa
    21301 cggcgacaat agcgaaacca cgcccatcag cgcgccatat gggcaaagat aacgacacca
    21361 ggcatggcga ataaacaggc tggcaatcag caaaacggtc acgctgatta atgtcgcggt
    21421 ccccatataa cgaaagaaat cgagcatttt aacgtccatc accacgctgt agggcgacaa
    21481 cataaaatag tgaatcgcct gagcgggcat caataacgcg atatagagaa aaaaactcaa
    21541 cagcaaatac tttacaccgc gcagaggaat atccagccag cggggaagga caaattgccg
    21601 accaaaaagt ttgttaccga gagcgccgat taattcagaa agcgtaccaa ccgggcataa
    21661 ccatgaacaa aaggcctttt tgagtaatag actgatgacg ataaaagcga ccaataacag
    21721 cattgcggcg gcgtggacgg acggtaactg acctgttgca aggctatatt tcagattcat
    21781 cagaccggca atcggtagcc agccttcgat acctcccggt ctggcgacaa atgtcgtact
    21841 acctgccgtt tcgtaatagc gcacccaata ccagaacgtg atggcaatat aaatattcat
    21901 tgccaatagt aagaattgcg tcgctttacg ccaggtcgtg gcattgcgcc agtcattcca
    21961 cggtaatttg ccgcccgtcg tgcctggccg ccgctgccag cgggttcttt ttttctctgc
    22021 catgattttg ccagtccgtt aagttgcata ccaaatgcca ctattctagt tattcttaac
    22081 tggctgatat tgattcaaat cgcgttcagg tctttcttat tcaaccatgc ttccagagcg
    22141 gcaacactgc gtgtaatttc ttcgtgtgga aggggggcag ataatggctg ctgctccagt
    22201 tgtgcgcata gctggctggc gctgcgcatt cccagactcg agcaactgct ttttagctga
    22261 tgcgcggcac gctttatttt ctcgctgtcc tggctggcgc gggcaatgtc gatttcatcg
    22321 agaagcggca gggcatgttg tttaaataat gccagccatt cgtggatctt ctccgtcccc
    22381 attaattgag catcttcatt gagttgcgat acatcaagtg gtatatcgtt attgacttgc
    22441 agttggagat agtgctccag taactgaccg agcacttcac gcggcaccgg tttagggata
    22501 atcccgcgaa atagtgaact ggtacgctgg cgcagcgttt cgtcaatgac atgagcgcta
    22561 aagccaatca aaaccagcga cggatattgc tgtgccagtt gccgggcaag ggtaatgccg
    22621 tcgatatccg gcagatcaaa atccaccagt gcggcagcaa acggttcgct attttgcagt
    22681 gtctctaaag cctgcgcggc attgccaata gcaacaacct gcgcaccact ggtgttcagc
    22741 atctcaacgg taattcgctg ggttagcggg ttatcttcaa ttaacagcaa acgtaaaccg
    22801 tccagacgca ccgcctgatt ggctgttttt ggcgcgggtg ctgtggcaac acgtaacggc
    22861 aagcgtaaac aaaaacagct tccaacctcc agcgtgctgg tggcgctcag ttcgccgccc
    22921 atcgcctggg ccagacggct actgatagtc agccccagcc cggtgccgcc gcgtttgcca
    22981 cttacctgaa taaatggctg gaagatttct gccagtttcg cgggatcaat accgcagccg
    23041 ctgtcttcca cttcgaccag ccattgctca ccatcagtac ggctacgtaa aacgatctgc
    23101 ccttcgtcag taaaacgcag ggcgttgctc aacaggttgg taatgacctg acgaatacgc
    23161 cgcggatcgc ccattaacgc agtcggtaca tcgtcggcaa ttgccgttgc caggcgaatc
    23221 gggcgacctt tcacccgtcc actcattaat tgcagggtac tttccagcag cggtcgcggt
    23281 tcaaagggtt catcgctgac cgaaacattc ttgccgcctg cttcgatagc cgaataatcg
    23341 agaatatcgt tcaggatggt cagcaacgat tcgccagagt cggtaatcgc ccgcaaatca
    23401 tcacgctggg cgttaagtgc ggggttatct gccagtagct gtgcggtgcc gagaataccg
    23461 tacagcggtg tgcggatctc atggctcatc gccgccagaa acgccgattt tgcctggctg
    23521 gctttttctg cttccgcccg tgcctgtcgg tgttctatca ccagttcctg caattcagct
    23581 gtacgtgctt tgacctgcgc cgccagctgt tcgcggtggc gattcagtgc atgaacactg
    23641 ctgcgaaacg catccatcag ccgcccgatg gtatccagct cccgtacgcc agcggtttcc
    23701 ggaaaagggg agtcaatatc accgtccagc agccgttgca gcgcctgcgt ttgttcggca
    23761 agtggacgcg tgactgagcg ataaaccacg cgccagagga tcagaatcag tgcgcaaagt
    23821 gaaaccatcc ccagcaataa caggctgtat tgcccgcgtg cactggcttt ttccagatgc
    23881 gccagtccat gctgattgcg cagctcaatg gtgtcgacca gctgactgac ttcgctacta
    23941 aactgcgcga actgggcgat attattttgg gcgagagttt gcaggtgatt gctgatttca
    24001 ctgtcctgct ggaacagcgc cagcaaatcg ctatattggc taacggtagt taacgttgtt
    24061 gcgacctgag cacgaactcc cggatcttca atgcgtattt gccgacgttg cagaattttc
    24121 accgcattat tgagctgctt ttccagcgtt ggcgcatttt tctggatctg ctccagcccc
    24181 agattcatca ccatttgctg cacccgcaga gcgctaaggc gcagttcatt catctggtta
    24241 acatactcaa gatcgatatc aatcagccga tcgagtgcac tttcagcagc ctgacgctga
    24301 tcttgttcga tcaaatcgta aatcccggcc tgggtcgctc cggcggaggt tgccgcatta
    24361 ttcgcctgac cttgcgccag gcgtgcgatc tcatcggcgg cagcgactat ctgctgactg
    24421 agtcgctgtt gttgctggcg cagttgcaaa cgctgcccca ccagttcccc ttgctgacgt
    24481 aacgaacggg agatctcctg ctcctgttgt tcaatagcgg tggtatcaaa accttgttcc
    24541 cgtaacgctt gcagcaacgc attaatcttc aggctttgtg cggtgagcat tcgcccctgc
    24601 gcctgccaca tcttttcgtt atcggcactg gtcaggttct gcgcggcgaa aagttcccag
    24661 gcgctggctt cgctcaactg gcgcgccata ttcatggtag gaatcaatgc ctgagtattg
    24721 tctttttcca cctggctgat aaagcgcagg ttgtaccatc ccaccagggt actggtcagg
    24781 gttaacagcg ccatcagggc aaagcccatc cagagtcttc gggtcagggt taaattcacg
    24841 gtcggtgcac ttaaggtgaa aaatgttgag tcgcaaagag gaatgcatct agcataaagc
    24901 cttattattg atgaggctat catgcgcgta ctgctatttt tacttctttc ccttttcatg
    24961 ttgccggcat tttcggctga taacctgttg cgctggcatg atgcgcagca tttcacggtg
    25021 caatcctcta cgccgcttaa agccaaacgc gcctggaaac tgtgcgcgct ttatcccagc
    25081 ctgaaagatt catactggtt atcgttgaac tatggtatgc aggaggctgc tcgccgctac
    25141 ggtgtggatt taaaagtgct ggaggcaggc ggctacagcc agttggctac ccagcaagca
    25201 caaatcgacc agtgtaaaca gtggggcgca gaggccattt tgctcggtag tagcacgacg
    25261 tcatttcccg atctgcaaaa gcaggtagca agtctgccgg tgatcgaact ggtaaatgct
    25321 attgatgctc cccaggtgaa aagccgcgtt ggtgtaccct ggtttcagat gggctatcaa
    25381 cctgggcgat atttggtgca atgggcgcac ggtaaaccac tgaacgtgct gttgatgccc
    25441 ggacccgata acgccggggg cagtaaggag atggtcgagg gttttcgcgc agccattgcc
    25501 ggaagcccgg tgcgtattgt tgatattgcg cttggtgata acgatattga aatccagcgt
    25561 aacctgttgc aggagatgct ggaacgccat ccagaaatcg acgtcgttgc cggaacggcc
    25621 attgcggcag aggcggcaat gggggaaggg cgtaacctga aaacgccgct taccgtggtg
    25681 tcgttttatc tttcacatca ggtgtatcgc gggctgaagc ggggaagagt gattatggct
    25741 gccagcgatc aaatggtctg gcagggggaa ctggcagttg agcaggccat caggcaatta
    25801 caggggcaat cggtgtctga taatgtcagc ccaccgattt tagttctgac gccgaaaaat
    25861 gccgaccgcg aacatatccg ccgctcgctg tcgccagggg gatttcgtcc ggtctatttt
    25921 tatcagcaca catcagcggc taagaaataa ccttcgccat gttgcgtcac cagtaaatcc
    25981 gcgctgagtt tatggcgtaa acgacgaatt aacacatcga cggtgcgcag gtcagggttt
    26041 tccacccgac gcgcagagag catacgaagc agacgttcac ggctgagaat ttctcccgga
    26101 ttggtcacaa atgccaccaa catttcatac tctgcgcggg tcagtttaat cggctcacca
    26161 tcccgctcca gcgtatggcg cgacacattc aggcaataac ccgcaaagcg atagcagttg
    26221 tcctgagtga gcggttgaac ttgtcgcgcg aggtcgattc gccagagcag attttttacc
    26281 cgtactacca gttcgcgcag ttccagcggt ttggtgacgt aatcgtctgc gcccatttcc
    26341 agcccaacaa tacggtcaat ccgatcgctg cgtccggtaa ccagaataat ccccaccgtt
    26401 gagcgttctc gcagggcgcg ggttaacatc aggccatttt catcgggcaa gttgatatcc
    26461 agcagaatta aatccaccgg ctgattctgc ataatttccc gtagcccggc accgctcgcc
    26521 gtaacggaaa cggtataccc ctcctgagtg aagtaggatt gtaatcgcgc ctgggtaacc
    26581 ggctcatctt caacaataac aatgtgatgt ggcatcagag ggttttactc attctgttca
    26641 tatctgttca tattctgccg taagccattc atcctgacca gtgccgctgt tcatatttgc
    26701 tcattaagat cgcttcacta aaccataatt ttacaggggc tattatgcgg aaactctgga
    26761 acgcgctacg ccgacccagt gctcgttggt cggtactggc gctggtcgct attgggattg
    26821 tgattggcat tgcgctgatt gtattgccac acgttgggat caaagtcacc agcacaaccg
    26881 aattttgtgt cagttgccac agtatgcaac cggtgtatga agaatataaa cagtcggtgc
    26941 atttccagaa cgcctccggc gtgcgagctg aatgccatga ctgtcatatc ccgccggata
    27001 ttccaggcat ggtgaagcgt aaactggaag cgagcaacga tatctaccag acctttattg
    27061 cccactccat tgatacacct gaaaaattcg aagccaaacg cgcggaactt gccgagcgtg
    27121 aatgggcacg aatgaaagaa aacaactcgg caacctgccg ctcctgccat aactacgacg
    27181 cgatggatca tgcgaagcag catcctgaag cggcacgtca gatgaaggtg gcagcgaaag
    27241 ataatcaatc ctgcatcgac tgtcataaag gtattgccca ccagttaccg gatatgagta
    27301 gcggcttccg taagcagttc gatgagctgc gcgccggtgc taatgacagt ggcgacaagc
    27361 tgtactctat tgatattaag ccgatttatg cggcgaaagg cgataaagaa gcctctggtt
    27421 ctctgctgcc tgcctcggaa gtgaaagtcc ttaaacgtga cggcgactgg ctgcaaattg
    27481 aaattaccgg ctggacagaa agcgccggac gtcagcgtgt actcacccaa ttcccaggta
    27541 aacgcatctt tgttgcctcg attcgtggtg atgtgcagca gcaggtgaaa acgctggaga
    27601 aaaccaccgt agccgacacc aataccgagt ggagcaagtt gcaggccacc gcgtggatga
    27661 agaaaggcga catggtgaac gatatcaaac cgatctgggc ttatgcggat tcgctgtaca
    27721 acggcacctg taaccagtgc cacggcgcac cggaaatctc ccactttgac gctaacggtt
    27781 ggatcggcac gctcaacggc atgattggct ttaccagcct cgataagcgt gaagaacgca
    27841 ccttgttgaa atatctgcaa atgaatgcgt ctgacaccgc aggtaaggct cacggcgata
    27901 agaaggaaga aaaataatga acaataacga tctctttcag gcatcacgtc ggcgttttct
    27961 ggcacaactc ggcggcttaa ccgtcgccgg gatgctgggg ccgtcattgt taacgccgcg
    28021 ccgtgcgact gcggcgcaag cggcgactga ggctgtcatc tcgaaagagg gcattcttac
    28081 cgggtcgcac tggggggcta tccgcgcgac ggtgaaggat ggtcgctttg tggcggcaaa
    28141 accgttcgaa ctggataaat atccgtcgaa aatgattgcc ggattgccgg atcatgtaca
    28201 caacgcggcg cgtattcgtt atccgatggt acgcgtggac tggctgcgta agcgccatct
    28261 gagcgacacc tcccagcgcg gtgataaccg ttttgtgcgt gtgagctggg atgaagccct
    28321 cgacatgttc tatgaagaac tggaacgcgt acagaaaact cacgggccga gtgccttgct
    28381 gaccgccagt ggttggcaat cgacggggat gttccataac gcttcgggga tgctggcgaa
    28441 agctattgcc ttgcatggta atagcgttgg tacgggcgga gattactcta ctggtgctgc
    28501 gcaggtgatc ctgccgcgcg tagtcggttc gatggaagtg tatgaacagc aaacctcctg
    28561 gccgctggta ttgcagaaca gcaaaaccat tgtgctgtgg ggctccgatt tgctgaaaaa
    28621 ccagcaagcg aactggtggt gcccggatca cgatgtttat gaatattacg cgcagttgaa
    28681 agcgaaagtc gccgccggtg aaattgaggt cattagcatc gatccggttg ttacatccac
    28741 ccatgagtat ctggggcgcg agcatgtgaa gcacattgcg gttaacccgc aaactgacgt
    28801 gccgctgcaa ctggcgctgg cgtatacgct gtacagtaaa aacctgtacg ataaaaactt
    28861 cctcgctaac tactgtgtgg gttttgagca gttcctgccg tatctgctgg gtgagaaaga
    28921 cggtcagccg aaagatgccg catgggctga aaaactgacc ggcattgatg ccgaaaccat
    28981 tcgtggactg gcgcggcaga tggcggcgaa cagaacgcag attattgctg gctggtgcgt
    29041 acagcgtatg cagcacggtg aacagtgggc gtggatgatt gtggttctgg cggcgatgct
    29101 ggggcaaatt ggcctgccag gtggtggctt tggttttggc tggcactata acggcgcagg
    29161 cacgccgggg cgtaaaggcg ttattctgag tggtttctcc ggctctacgt cgattccgcc
    29221 tgttcacgac aacagtgatt acaaaggcta cagcagcacc attccgattg cccgttttat
    29281 cgatgcgatc ctcgaaccgg ggaaagtgat caactggaac ggtaaatcgg taaaactgcc
    29341 gccgctgaaa atgtgtattt ttgccggaac taacccattc catcgccatc agcagatcaa
    29401 ccgcattatt gaaggctggc gcaagctgga aacggttatc gccatagata accagtggac
    29461 ctcaacctgc cgctttgccg atatcgtgct gcctgcgacc acgcagtttg agcgtaacga
    29521 tctcgaccag tacggcaacc actccaaccg tggcattatc gccatgaaac aggtcgtgcc
    29581 gccgcagttc gaggcgcgca acgactttga tattttccgc gagctgtgcc gccgctttaa
    29641 tcgcgaagaa gcctttaccg aagggctgga cgaaatgggc tggctgaaac gcatctggca
    29701 ggaaggtgtt cagcaaggca aaggacgcgg cgttcatttg ccagcgtttg atgacttctg
    29761 gaataacaaa gagtacgtcg agtttgacca tccgcagatg tttgttcgcc accaggcatt
    29821 ccgcgaagat ccggatctcg aaccgctggg cacgccgagt ggcctgattg agatctactc
    29881 gaaaaccatc gccgatatga actacgacga ttgtcagggg catccgatgt ggtttgagaa
    29941 aatcgaacgc tcccacggtg ggcccggctc gcagacgtat ccgttgcatc tgcaatctgt
    30001 gcatccggat ttccgacttc actcgcagtt atgtgagtcg gaaactctgc gtcagcaata
    30061 tacgatagcg ggtaaagagc cagtgttcat taacccgcag gatgccagcg cgcgcggtat
    30121 tcgtaacggt gatgtggtac gcgtctttaa cgctcgcggt caggtgttgg caggggcagt
    30181 agtttctgat cgctatgcac ccggcgtggc gcgaattcac gaaggggcat ggtacgatcc
    30241 agataaaggc ggcgagcctg gtgcgctgtg caaatacggt aaccccaacg tgttgacaat
    30301 cgacatcggt acttcgcagc tggcgcaggc gaccagtgcg cacactacgc tggtggaaat
    30361 tgagaagtac aacggaacag tggatcaggt gacggcgttt aacggccccg tggagatggt
    30421 ggcgcagtgc gaatatgttc ccgcgtcgca ggtgaaatca tgaccacgct gacagcacaa
    30481 cagattgcct gtgtttacgc ctggctggcg cagttgttct cccgtgaact ggacgatgaa
    30541 caactgacgc aaatcgccag tgcgcagatg gctgaatggt tttcgttgct gaaaagcgaa
    30601 ccgccgctca ctgcggcgat ggatgagctg gaaaaccgtg ttgccactct gacagtacgt
    30661 gacgatgccc gtctggaact ggccgctgac ttttgcggcc tgtttctgat gactgacaaa
    30721 caagcggcgc tgccgtatgc atcggcctac aaacaggacg agcaagaaat taaacgcttg
    30781 ttagttgagg cagggatgga aaccagcggc aatttcaacg aaccggcaga tcatctggcg
    30841 atctatctcg agttgttgag tcatctgcat ttttcgctgg gagaggggac ggttcctgcg
    30901 cgaagaatcg acagtttacg gcaaaaaaca ctcacggcgc tgcggcaatg gttaccagag
    30961 tttgttgcgc gttgccatca gtatgacagc tttggttttt acgcggcact aagccagtta
    31021 ttgctggtgt tagttgaggg cgaccaccaa aacagataac gtcgtttgtt ggcctgataa
    31081 gacgcgttta gcgtcgcatc aggcattatg gggcagttgc cggatgcgtc atcacatccg
    31141 gcaaacacac atcacggatg agctacaaac cgggaaagcc gctggcgcag caggcggttt
    31201 tcctgcttca ggtgcgcaat atcatccatt aacgtcagcg ccaccgcgat ccccggccag
    31261 tccagagcca gttcatgacg caggcgcacc gcgcgttgca ccacgatggc tgcatggtcg
    31321 tcaaataccc aggttgtttc ctggatctca cgcggttcaa ccacccccaa accgacaatt
    31381 tcattcagtt cctcttcaga gatgccggta tgcaggcaaa attcggtaat agtaaaagtc
    31441 accgtaacat tagccattat gctttccccc aatctttacg tggatcaaaa gacgactggg
    31501 cgtctgccag ttgctgccac aacgcggcag tgttttcatc cggtttcggc ggcatcacga
    31561 ttttcagtac cgcatacaga tcgccggtct gttttttgct caccagacct ttgcctttaa
    31621 cgcgcaatcg ttgcccggcc tggctgccag gcgggatagt cagcaaaatg ctttctttca
    31681 gtgttggaac ggtgacttta gcacccagcg ccgcttccca cgggctaacc ggcaccacaa
    31741 tttccagatc ctggccgaca atatcaaaca gcggatgtgg cgcaatatga atcaccagcc
    31801 acaaatcgcc atttggaccg ccgttttcgc ccggcgtccc ctggcctttc aggcggatgc
    31861 gttgaccatt gccgacgcct gccgggatct tcacattcag cgttttcggg atttcctgtt
    31921 cgatcatgcc aaaggcgtta taaaccggca ggttatagct gatggtacgc ttatgctcag
    31981 taagcgtttc ttcgaggaat accgccactt cgatttcaat atcgtggccg cgtgtggcgg
    32041 ggcgttgacg gctctggcgg gcatgctgac cgaaaattga cgagaagata tcgtcaaaat
    32101 cttcggcgtt aaaactctga ccgtcgccat ggtggaactg acggttaaat tgcggatcgt
    32161 tgcgatgttg ccacatctga tcatactcag cgcgacgttg ttcatcactt aacacttccc
    32221 aggcttcagc gacctctttg aagcgggctt cggcatccgg ttctttgctg acatcaggat
    32281 ggtatttgcg ggcaagtcga cgataggcgg tcttgattgt cttgagatcg tccgtcggtt
    32341 tcacgcccat gatggcgtaa taatccttta attccatagc gttatctcgc gtaaatcaac
    32401 acaaattgaa ggaacccctg taaggtaact cctataagtg tagggtaatc ctcaaaattt
    32461 catatgccaa cacagaatat gttattgaaa tcatcgcgga gaggaggtcg cctcaaggtg
    32521 gggtgctgaa catattttaa acaggtgaaa aagggtgagc gatttttgat agttgaaaca
    32581 ggcactttaa gttttactag tgcgtcatta tttattaaat tttataggcg ctatatatgg
    32641 gtaatataca tggaattagt tgcactgcaa ataattattt gaagcaagcc tggaacgata
    32701 taaaaaatga gtacgaaaaa aataaaacat attcaatcac gctttttgaa aacacactgg
    32761 tgtgttttat gcggttgtat aaagaaatca gacgtcaaca agctgaagac tacattccat
    32821 gcctggaatg cgattcacta gaaaaagaat ttgaggaaat acagaatgat aatgatttat
    32881 cattatttat gaaaacattg cgtactaatg ataaacaaat tt
//
LOCUS       DAZFKF010000050        32199 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFKF010000050 DAZFKF010000000
VERSION     DAZFKF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.050"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(65..1825)
                     /gene="ycaO"
                     /locus_tag="ACHKA1_002799"
     CDS             complement(65..1825)
                     /gene="ycaO"
                     /locus_tag="ACHKA1_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO5680424.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSNNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRDTILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1955..2647
                     /locus_tag="ACHKA1_002800"
     CDS             1955..2647
                     /locus_tag="ACHKA1_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO5680425.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2846..3934
                     /gene="serC"
                     /locus_tag="ACHKA1_002801"
     CDS             2846..3934
                     /gene="serC"
                     /locus_tag="ACHKA1_002801"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO5680426.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNVLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4005..5288
                     /gene="aroA"
                     /locus_tag="ACHKA1_002802"
     CDS             4005..5288
                     /gene="aroA"
                     /locus_tag="ACHKA1_002802"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO5680427.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKIIYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5457..6221
                     /gene="ycaL"
                     /locus_tag="ACHKA1_002803"
     CDS             5457..6221
                     /gene="ycaL"
                     /locus_tag="ACHKA1_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO5680428.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6394..7077
                     /gene="cmk"
                     /locus_tag="ACHKA1_002804"
     CDS             6394..7077
                     /gene="cmk"
                     /locus_tag="ACHKA1_002804"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO5680429.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7188..8861
                     /gene="rpsA"
                     /locus_tag="ACHKA1_002805"
     CDS             7188..8861
                     /gene="rpsA"
                     /locus_tag="ACHKA1_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO5680430.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9021..9305
                     /gene="ihfB"
                     /locus_tag="ACHKA1_002806"
     CDS             9021..9305
                     /gene="ihfB"
                     /locus_tag="ACHKA1_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO5680431.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9512..11776
                     /locus_tag="ACHKA1_002807"
     CDS             9512..11776
                     /locus_tag="ACHKA1_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO5680432.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILLAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQLTVNFSAQGWLISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11813..13561
                     /gene="msbA"
                     /locus_tag="ACHKA1_002808"
     CDS             11813..13561
                     /gene="msbA"
                     /locus_tag="ACHKA1_002808"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO5680433.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13558..14544
                     /gene="lpxK"
                     /locus_tag="ACHKA1_002809"
     CDS             13558..14544
                     /gene="lpxK"
                     /locus_tag="ACHKA1_002809"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO5680434.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVVDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14581..15813
                     /gene="ycaQ"
                     /locus_tag="ACHKA1_002810"
     CDS             14581..15813
                     /gene="ycaQ"
                     /locus_tag="ACHKA1_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO5680435.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQRQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGHC
                     PQGLFTDCRTGWEIDPVA"
     gene            15865..16047
                     /gene="ycaR"
                     /locus_tag="ACHKA1_002811"
     CDS             15865..16047
                     /gene="ycaR"
                     /locus_tag="ACHKA1_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO5680436.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16044..16790
                     /gene="kdsB"
                     /locus_tag="ACHKA1_002812"
     CDS             16044..16790
                     /gene="kdsB"
                     /locus_tag="ACHKA1_002812"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO5680437.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLKRVRAEMR"
     gene            16944..17837
                     /locus_tag="ACHKA1_002813"
     CDS             16944..17837
                     /locus_tag="ACHKA1_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO5680438.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17814..18593)
                     /gene="elyC"
                     /locus_tag="ACHKA1_002814"
     CDS             complement(17814..18593)
                     /gene="elyC"
                     /locus_tag="ACHKA1_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO5680439.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLSLLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRDNPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            18729..19514
                     /gene="cmoM"
                     /locus_tag="ACHKA1_002815"
     CDS             18729..19514
                     /gene="cmoM"
                     /locus_tag="ACHKA1_002815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO5680440.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19511..20833
                     /gene="mukF"
                     /locus_tag="ACHKA1_002816"
     CDS             19511..20833
                     /gene="mukF"
                     /locus_tag="ACHKA1_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO5680441.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20814..21518
                     /gene="mukE"
                     /locus_tag="ACHKA1_002817"
     CDS             20814..21518
                     /gene="mukE"
                     /locus_tag="ACHKA1_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO5680442.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21518..25978
                     /gene="mukB"
                     /locus_tag="ACHKA1_002818"
     CDS             21518..25978
                     /gene="mukB"
                     /locus_tag="ACHKA1_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO5680443.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVEQ
                     RHLADQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNNDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26239..28086
                     /gene="ldtD"
                     /locus_tag="ACHKA1_002819"
     CDS             26239..28086
                     /gene="ldtD"
                     /locus_tag="ACHKA1_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5680444.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGILPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQQALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28267..28815
                     /locus_tag="ACHKA1_002820"
     CDS             28267..28815
                     /locus_tag="ACHKA1_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO5680445.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28842..29489
                     /gene="gloC"
                     /locus_tag="ACHKA1_002821"
     CDS             28842..29489
                     /gene="gloC"
                     /locus_tag="ACHKA1_002821"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO5680446.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLKEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29540..30730)
                     /gene="aspC"
                     /locus_tag="ACHKA1_002822"
     CDS             complement(29540..30730)
                     /gene="aspC"
                     /locus_tag="ACHKA1_002822"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO5680447.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30915..31988)
                     /gene="ompF"
                     /locus_tag="ACHKA1_002823"
     CDS             complement(30915..31988)
                     /gene="ompF"
                     /locus_tag="ACHKA1_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO5680448.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQKGNK
                     TRLAFAGLKFADAGSIDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERAGIPERSNGDGVGGSISYEFEGFGIVGAYGAAD
                     RTDAQEAEFRGQGKKAEQWATGLKYDANNIYLAANYGETRNATPIEGGFANKTQDVLL
                     VAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVDYIINQ
                     IDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7831 a         7859 c         8666 g         7843 t
ORIGIN      
        1 aaaaaacgcc ccgtaatata actcagacga atcattaagc ctacattgcg taggctattt
       61 gattttattt tgcccagaat gctgctttgg cgcgctgcag cttttcgtag gccttcaaca
      121 gcgactgatg tgcagcaaac gcgtgcagat cgctatctac tggttgcaag ccataaaacg
      181 ccgcttcgcc gctcattgcc gcactagcgg cttccacggc atctgcgccg tacatgcgaa
      241 caaacgcatt cagatattgc agcggctggc gatcttcttc ctgcgccagt aataacaacg
      301 tttgcaggca gcgataatag ttggcgcgtt ccgggctgaa taccgatgag ttaaactcca
      361 tcgtccattc ggtccagacc agagcctgtt ccagatcgcc accagccagc gccagcatgg
      421 cttttaattc accgatacgc agggtgtacc aaccgttatc cgacccggtc gccagaccca
      481 acagctcacg cacgcgggta aagtcatcaa aaccttcttc atccagttgc tcgatgaggt
      541 tcaggtaatc ttctttttcc cactcgctgc ctggtagcga gaggatggtg tcgcgcagat
      601 gactacccat actgttattc gctaaccaca aatcttcagc cggataaata tcggacatgc
      661 caggcacgat aatacggcaa gcataaacgc ccagatgctc gtaatcggca atataaactt
      721 ctttatcttc tttgttgaag atagccatca gcgtggcaaa ctcttcttcc gtggtgccgg
      781 agaaattcca gtccacaaac ggataatcgg catcctgctt gaacaggtcc caggagatta
      841 aaccgctgga atcgataaag tgcgtttcga ggttggtatg ttcagcgact tcttcatcat
      901 cgaaggttgg cggagtaaac acatccaaat ctttcaggcc acgaccttgc agcagctcgg
      961 tcacggtacg ttccagtgct acgccaaaat caggatgcgc accgaaagag gcaaagcagg
     1021 tgccgttagc aggattaaac agtaccacgc aaatcaccgg atactggccg ccaagcgaac
     1081 cgtcataagc gaagatcggg aaaccttccg cttccagcgt ttcgatcgct tcaactactg
     1141 ctggataacg tgccagcacg tctgccggga tttccggcaa gctgatgctt tcagcaataa
     1201 tgcggttttt cacgtagcgt tcgaaaactt cggacaaccc ctgaacgcgt gcttcgttac
     1261 gggtattgcc agcagacata ccgttagaaa cgtacaagtt accaatgata ttcatcggaa
     1321 tataaacggt ctgattatta gactgacgtg taaacggcag accgcaaata ccacgatctt
     1381 cgttaccgga ttgtaggtca atcagcatac tgccggtcaa ttcattctcc ggatcgtaaa
     1441 atgcgcgcag acggtcatcg agcagccctt ctggcacatc gtcattttcg gtcagtggga
     1501 accatttttc gttgggataa tgcacaaatg gaccgttggc gatggtttcg cccagccaga
     1561 aatcagcaaa aaagtagttg gttgagagac gctcgaaata ttcaccgagt gcagaagcca
     1621 gcgccgcttt cttggttgcg cctttaccgt tggtaaaaca cagtgcgcac tctttgtcgc
     1681 gaatatgtac agaccagacg ttaggcacgg gattcagcca ggaggcctct tcaatctgaa
     1741 agccgaggtc tgaaagtttt tgctggaagc gagcgatgga atcttccagt gcggcatctt
     1801 tgccggggat aaatgtttgc gtcatgaaaa tcactttagt cgtacggaaa gcgcgcaata
     1861 atacgggttt tatctcaaag gcgctatcac cgccgccatg ccgatgaact gttgactatg
     1921 cttttagcgg ataaaccacg ttaagagcat aaaaatgaag gcgttcgatc tccaccgtat
     1981 ggcatttgat aaagtgcctt ttgatttcct tggcgaagtt gcactacgta gtctgtatac
     2041 ctttgtactg gtttttttgt tcctcaaaat gaccggaaga cgcggagtgc ggcagatgtc
     2101 gctgtttgaa gtgttaatca ttctgacgct ggggtcagcg gcgggagatg tggcgtttta
     2161 tgatgatgtg ccgatggtcc cggtacttat cgtctttatc actctggcgt tgttataccg
     2221 cctggtaatg tggttgatgg cacacagtga aaaactggaa gatcttctgg aaggtaagcc
     2281 ggttgtcatt attgaagatg gcgagctggc ctggtcgaaa ctcaataact ccaacatgac
     2341 ggaatttgag ttctttatgg agctacgatt gcgcggtgta gagcagttgg ggcaagtacg
     2401 tctggcgatt ctcgaaacca acgggcaaat cagtgtctat ttctttgaag atgacaaggt
     2461 gaaaccgggt ttacttattt tacccagtga ttgtacgcag cgttacaaag tggtgccgga
     2521 gtcggcggac tatgcctgca tccgttgtag tgaaatcatt catatgaacg cgggggaaaa
     2581 acaattatgt ccgcgctgtg caaatccaga atggacgaag gcaagtcggg ctaaacgggt
     2641 gacctgacag caaaaatatc ggttttttgc taataatccg agagattctg ttgtgtgacg
     2701 cgtgccgcat ttttgccctc aacggtttta cccattgcga tgcgtgtcac tgaatgataa
     2761 aaccgatagc cacaggaata acatattacc cgcggtcgca atcgattgac tgggggttaa
     2821 tggcaacgca acgtggtgag gggaaatggc tcaaatcttc aattttagtt ctggtccggc
     2881 aatgctaccg gcagaggtgc ttaaacaggc tcaacaggaa ctgcgtgact ggaacgttct
     2941 tggtacgtcg gtgatggaag tgagtcaccg tggcaaagag ttcattcagg ttgcagagga
     3001 agccgagaag gattttcgcg atcttcttaa tgtcccctcc aactacaagg tattgttctg
     3061 ccatggcggc ggtcgcggtc agtttgctgc tgttccgctg aatattctcg gtgataaaac
     3121 caccgcagat tatgttgatg ccggttactg ggcggcaagt gccattaaag aagcgaaaaa
     3181 atactgcacg cctaatgtct ttgacgccaa agtgactgtt gatggtctgc gcgcggttaa
     3241 gccaatgcgt gaatggcaac tctctgataa tgctgcttat atgcattact gcccgaatga
     3301 aaccatcgac ggtatcgcca tcgacgaaac gccagacttc ggcaaagatg tggtggtcgc
     3361 cgccgacttc tcttcaacca ttctttcccg tccgattgac gttagccgtt atggcgtgat
     3421 ttacgctggc gcgcagaaaa atatcggccc ggcaggcctg acaatcgtca tcgttcgtga
     3481 agatttgctg ggcaaagcga atatcgcgtg tccgtcgatt ctggattatt ccatcctcaa
     3541 cgataacggc tccatgttta acacgccgcc gacatttgcc tggtatctgt ctggtctggt
     3601 ctttaaatgg ctgaaagcga acggcggtgt agctgaaatg gataaaatca atcagcaaaa
     3661 agcagaactg ctatatgggg taattgataa cagcgatttc taccgcaatg acgtggcgaa
     3721 agctaaccgt tcgcggatga acgtgccgtt ccagttggcg gacagtgcgc ttgacaaatt
     3781 gttccttgaa gagtcttttg ctgcaggcct tcatgcactg aaaggtcacc gtgtggtcgg
     3841 cggaatgcgc gcgtctattt ataacgccat gccgctggaa ggcgttaaag cgctgacaga
     3901 cttcatggtt gagttcgaac gccgtcacgg ctaatgccga aattttgctt aatccccaca
     3961 gccagcctgt ggggttttta tttctgttgt agagagttga gttcatggaa tccctgacgt
     4021 tacaacccat cgctcgtgtc gatggcacta ttaatctgcc cggttccaag agcgtttcta
     4081 accgcgcttt attgctggcg gcattagcac acggcaaaac agtattaacc aatctgctgg
     4141 atagcgatga cgtgcgccat atgctgaatg cattaacagc gttaggggta agctatacgc
     4201 tttcagccga tcgtacacgt tgcgaaatta tcggtaacgg cggtccatta cacgcagaag
     4261 gtgccctgga gttgttcctc ggtaacgccg gaacggcaat gcgtccgctg gcggcagctc
     4321 tttgtctggg tagcaatgat attgtgctga ccggtgagcc gcgtatgaaa gagcgcccaa
     4381 ttggtcatct ggtggatgcg ctgcgcctgg gcggggcgaa gatcatttac ctggaacaag
     4441 aaaattatcc gccgttgcgt ttacagggcg gctttactgg cggcaacgtt gacgttgatg
     4501 gctccgtttc cagccaattc ctcaccgcac tgttaatgac tgcgcctctt gcgccggaag
     4561 atacggtgat tcgtattaaa ggcgatctgg tttctaaacc ttatatcgac atcacactca
     4621 atctgatgaa gacgtttggt gttgaaattg aaaatcagca ctatcaacaa tttgtcgtaa
     4681 aaggcgggca gtcttatcag tctccgggta cttatttggt cgaaggcgat gcatcttcgg
     4741 cttcttactt tctggcagca gcagcaatca aaggcggcac tgtaaaagtg accggtattg
     4801 gacgtaacag tatgcagggt gatattcgct ttgctgatgt gctggaaaaa atgggcgcga
     4861 ccatttgctg gggcgatgat tatatttcct gcacgcgtgg tgaactgaac gctattgata
     4921 tggatatgaa ccatattcct gatgcggcga tgaccattgc cacggcggcg ttatttgcaa
     4981 aaggcaccac cacgctgcgc aatatctata actggcgtgt taaagaaacc gatcgcctgt
     5041 ttgcgatggc aacagaactg cgtaaagtcg gtgcggaagt agaagagggg cacgattaca
     5101 ttcgtatcac tccaccggaa aaactgaact ttgccgagat cgcgacatac aatgatcacc
     5161 ggatggcgat gtgtttctcg ctggtggcgt tgtcagatac accagtgacg attcttgatc
     5221 ccaaatgtac ggccaaaaca tttccggatt atttcgagca gctggcgcgg attagccagg
     5281 cagcctgaat gaataacgtc caataaatag ccaaatcttt ctttatcaaa acgccggcac
     5341 attgccggcg ttttttttcg gaccttgtga gtcattttga ttaatggtag cgtcgcttgt
     5401 caatgtaagt tgttgataca taatatttat atatgattaa tcaacggatg attcacatga
     5461 agaatactaa attactgctg gcgattgcga cctctgcagc attactgaca gggtgtcaaa
     5521 atacccacgg tattgatacc aatatggcta tcagctccgg tttaaatgcc tataaagcag
     5581 caacgttaag cgatgccgat gcaaaagcga ttgccaatca gggctgtgcc gaaatggaca
     5641 gtggcaatca agtcgcaagt aaatccagca agtacggtaa acgtctggca aaaatcgcca
     5701 aagcattggg taacaatatt aatggcacgc cggtcaacta taaggtttat atgaccagcg
     5761 acgtcaacgc atgggcgatg gcgaacggct gtgttcgtgt ctacagtggc ctgatggaca
     5821 tgatgaatga caacgaaatt gaaggtgttc tgggccatga actgggccac gtcgcgttgg
     5881 gtcactcgct ggctgaaatg aaagcttctt atgcgatcgt tgccgcacgc gatgccattt
     5941 cagctaccag cggtgtggct tcccagcttt cccgctcaca attaggtgat atcgcagaag
     6001 gcgctatcaa tgcgaaatac tcccgtgata aagagtccga agcagatgat ttctcctttg
     6061 atctgttgaa gaaacgtggc atcagcaccc aggggctggt tggcagcttt gaaaaactgg
     6121 ctagcctgga tggcggtcgc acccagtcca tgtttgactc tcacccacca tcaacagagc
     6181 gtgcgcaaca catccgtgat cgtatcgcct ctggtaagta aatcattgtc atctttcggg
     6241 ctggtcttct gccagcccgc tataattgcg caataaatcc ccatctgaat acagacaaaa
     6301 ctggtttttg cacacaacgt taacgatttg tggcgtcggc gcgtataatg cgcgcggtta
     6361 tgttaacggt acgcctgttt taaggagata aagatgacgg caattgcccc ggttattacc
     6421 attgatggcc caagcggtgc agggaaaggc accttgtgta aggctatggc ggaagcgttg
     6481 caatggcatc tgctggactc gggtgcaatt tatcgcgtac tggcactagc ggcattacat
     6541 caccatgttg atgttgcttc ggaagatgcg ctggtgccgc tggcatccca tctggatgta
     6601 cgttttgtgt cgaccaatgg caatctggaa gtgatcctcg aaggggaaga tgtcagcggc
     6661 gaaattcgta ctcaggaagt ggcgaatgct gcttcacaag tcgcggcatt cccacgcgtt
     6721 cgtgaagcat tattgcgtcg ccaacgcgcg tttcgcgaat taccaggcct gattgccgat
     6781 ggccgcgaca tgggaacggt ggtattccct gatgcgccag tgaaaatttt ccttgacgcc
     6841 tcctcggaag aacgtgcgca tcgccgcatg ctacagttgc aggagaaggg ctttagtgtt
     6901 aactttgagc gccttttggc cgagatcaaa gaacgcgacg accgcgatcg taaccgagcg
     6961 gtagcgccac tggttccggc agccgatgct ttagtgttgg attccaccac cttaagcatt
     7021 gagcaagtga ttgaaaaagc gctacaatac gcgcgccaga aattggctct cgcataagcg
     7081 accgaatttg cagtaccccc gttgcaatgg aatgacagcg ggtatgttaa acaaccccat
     7141 ccggcatgga gccaggtgga cgttaaatat aaacctgaag attaaacatg actgaatctt
     7201 ttgctcaact ctttgaagag tccttaaaag aaatcgaaac ccgcccgggt tctatcgttc
     7261 gtggcgttgt tgttgctatc gacaaagatg tagtactggt tgacgctggt ctgaaatctg
     7321 agtccgccat cccggctgag cagttcaaaa acgcccaggg cgagctggaa atccaggtag
     7381 gtgacgaagt tgacgttgct ctggacgcag tagaagacgg cttcggtgaa actctgctgt
     7441 cccgtgagaa agctaaacgt cacgaagcct ggatcacgct ggaaaaagct tacgaagatg
     7501 ctgaaactgt taccggtgtt atcaacggca aagttaaggg cggcttcact gttgagctga
     7561 acggtattcg tgcgttcctg ccaggttccc tggtagacgt tcgtccggtg cgtgacactc
     7621 tgcacctgga aggcaaagag cttgaattta aagtaatcaa gctggatcag aagcgcaaca
     7681 acgttgttgt ttctcgtcgt gccgttatcg aatccgaaaa cagcgcagag cgcgatcagc
     7741 tgctggaaaa cctgcaggaa ggcatggaag ttaaaggtat cgttaagaac ctcactgact
     7801 acggtgcatt cgttgatctg ggcggcgttg acggcctgct gcacatcact gacatggcct
     7861 ggaaacgcgt taagcatccg agcgaaatcg tcaacgtggg cgacgaaatc actgttaaag
     7921 tgctgaagtt cgaccgcgaa cgtacccgtg tatccctggg cctgaaacag ctgggcgaag
     7981 atccgtgggt agctatcgct aaacgttatc cggaaggtac caaactgact ggtcgcgtga
     8041 ccaacctgac cgactacggc tgcttcgttg aaatcgaaga aggcgttgaa ggcctggtac
     8101 acgtttccga aatggattgg accaacaaaa acatccaccc gtccaaagtt gttaacgttg
     8161 gcgatgtagt ggaagttatg gttctggata tcgacgaaga acgtcgtcgt atctccctgg
     8221 gtctgaaaca gtgcaaagct aacccgtggc agcagttcgc ggaaacccac aataagggcg
     8281 accgtgttga aggtaaaatc aagtctatca ctgacttcgg tatcttcatc ggcctggacg
     8341 gcggcatcga cggcctggtt cacctgtctg acatctcctg gaacgttgca ggcgaagaag
     8401 cagttcgtga atacaaaaaa ggcgacgaaa tcgctgcagt tgttctgcag gttgacgcag
     8461 aacgtgaacg tatctccctg ggcgttaaac agctcgcaga agatccgttc aacaactggg
     8521 ttgctctgaa caagaaaggc gctatcgtaa ccggtaaagt aactgcagtt gacgctaaag
     8581 gcgcaaccgt agaactggct gacggcgttg aaggttacct gcgtgcttct gaagcatccc
     8641 gtgaccgcgt tgaagacgct accctggttc tgagcgttgg cgacgaagtt gaagctaaat
     8701 tcaccggcgt tgatcgtaaa aaccgtgcaa tcagcctgtc tgttcgtgcg aaagacgaag
     8761 ctgacgagaa agatgcaatc gcaactgtta acaaacagga agatgcaaac ttctccaaca
     8821 acgcaatggc tgaagctttc aaagcagcta aaggcgagta attctctgac tcttcgggat
     8881 ttttattccg aagattgttg agtttacttg acagattgca ggtttcgtcc tgtaatcaag
     8941 cactaagggc ggctacggcc gcccttaatc aatgcagcaa cagcagccgc ttaatttgcc
     9001 tttaaggaac cggaggaatc atgaccaagt cagaattgat agaaagactt gccacccagc
     9061 aatcgcacat tcccgccaag acggttgaag atgcagtaaa agagatgctg gagcatatgg
     9121 cctcgactct tgcgcagggc gagcgtattg aaatccgcgg tttcggcagt ttctctttgc
     9181 actaccgcgc accacgtacc ggacgtaatc cgaagactgg cgataaagta gaactggaag
     9241 gaaaatacgt tcctcacttt aaaccaggta aagaactgcg cgatcgcgcc aatatttacg
     9301 gttaagtttt tactcaaact tgaacgcgag aaaagcacct gtcgggtgct tttttcattt
     9361 ctctaatcag gaactggaag ctgcctcgca gagttttgag cagttttcac cctttcgtta
     9421 aattcttctg aatgtgcctc ggggaacgca aaattcccac cagacaaccg ctcaacaaag
     9481 ttgcacactt tccataaaca gggaggggtg catgaaaata acgacagtcg gcgtatgcat
     9541 aatttgcgga atttttccgt tgctgatttt gccccaattg cctgggacat taacccttgc
     9601 gtttctgact ctcttcgcct gcgtactggc atttatccct gttaaaaccg tccgttatat
     9661 cgcgctgacg ttgctgtttt tcgtttgggg catattatca gcaaagcaaa ttttgttggc
     9721 aggagaaacc ttaactggcg cgacgcagga tgcaattgtt gagatcactg ctactgacgg
     9781 catgaccact cattacggtc aaattactta tctacaaggt cgacgtatat tccctgcgcc
     9841 aggccttgtg ctgtatggcg aatatcttcc gcaagcggtt tgtgccggac aactgtggtc
     9901 aatgaaactc aaagttcgtg ccgttcatgg tcaacttaat gatggcggct ttgatagcca
     9961 gcgttatgcc attgcccagc atcagccgct caccggccgc tttctgcagg caagtgttat
    10021 tgaaccgaat tgtagcctgc gtgcacagta tctggcgtca ctacaaacaa cgctgcaacc
    10081 ctatccgtgg aatgcggtta ttcttggttt aggtatgggg gaacggttat ccgtccctaa
    10141 agaaatcaaa aatatcatgc gtgatactgg aacggcgcat ttaatggcga tatcgggatt
    10201 gcacatcgct tttgcggcgt tgctggctgc cggactcatt cgcagtggac aaatttttct
    10261 gcctgggcgc tggatccact ggcaaatgcc attaattggc ggaatctgct gtgctgcttt
    10321 ttatgcctgg ctgacgggaa tgcaacctcc tgcattgcgt accgttgtgg cgcttgctac
    10381 gtggggaatg cttaagttaa gtgggcgaca gtggagtggc tgggatgtat ggatatgttg
    10441 tctggcggca attttgctga tggatcctgt tgccattctc tcgcaaagtt tatggctctc
    10501 tgccgctgcg gtcgcggcat tgatattttg gtatcagtgg tttccctgtc ctgagtggca
    10561 actgccgccg gtattgcgtg cagttgtttc cctcattcat ttgcaactgg gaatcacact
    10621 tctgcttatg cccgtgcaaa tcgtcatatt tcatggcatt agtctgacct cgtttattgc
    10681 aaatctatta gcaattccct tggtgacatt tatcacggtt ccgctgatcc tcgccgcgat
    10741 ggttgtgcat ttaagcgggc cgttaatcct ggagcaaggg ttatggtttc ttgccgaccg
    10801 gtctttggct ttacttttct gggggttaaa gagtttgcca gaagggtgga tcaacattgc
    10861 tgaacgttgg caatggctat cattttcccc atggttctta ctggtggtat ggcgattaaa
    10921 cgcctggcga acgttgccag caatgtgtgt ggctgtaggc ttgctgatgt gctggccgct
    10981 gtggcaaaaa cctcgacctg acgagtggca agtgtacatg cttgatgtcg ggcaagggct
    11041 ggcaatggtg atagccagaa acggcaaagc gattctctat gacacgggac tggcctggcc
    11101 cgaaggggat agtgggcaac aactgattat cccctggctc cactggcata atcttgaacc
    11161 ggaaggcgtt attctgagtc atgaacatct ggatcaccgg ggagggctgg actcaatatt
    11221 gcacacatgg ccgatgttat ggatcagaag tccgttaaac tgggaacatc atcagccctg
    11281 tgtgcgtggc gaagcgtggc aatggcaagg attgcgtttc agcgtgcact ggcctttaca
    11341 agctagcaac gataaaggaa ataaccattc ctgtgtggtt aaggttgatg acgggacgaa
    11401 tagcattctt ctaaccggtg atattgaagt ccccgctgaa caaaagatgc taagccgtta
    11461 ctggcagcaa gtgcaggcaa cattgcttca ggtacctcac catggcagta atacctcatc
    11521 atcgttgcca ttaattcagc gagtgaatgg aaaagtggca ctcgcatcgg catcgcgcta
    11581 taacgcatgg cgactgccct ctaacaaagt taagcatcgc tatcaacaac agggttatac
    11641 gtggcttgat actcctcatc aggggcaact aacggtcaat ttttcagcac aaggctggct
    11701 gattagcagc ctcagggagc aaattttacc tcgttggtat catcagtggt ttggcgtgcc
    11761 agtggataac gggtagaata tgcggctatt tcaacaaatg ctggtttttt gaatgcataa
    11821 cgacaaagat ctctctacgt ggcagacatt ccgccgactg tggccaacca ttgcgccttt
    11881 taaagcgggt ctgatcgtgg cgggcgtagc gttaatcctc aacgcagcca gcgatacctt
    11941 catgttatcg ctccttaagc cacttcttga tgatggcttt ggtaaaacag atcgctccgt
    12001 gctggtgtgg atgccgctgg tggtgatcgg gctgatgatt ttgcgtggta tcaccagcta
    12061 tgtctccagc tactgtatct cctgggtatc aggaaaggtg gtaatgacca tgcgtcgccg
    12121 cctgtttggt cacatgatgg gaatgccggt ttcattcttt gacaaacagt caacgggtac
    12181 gctgttgtca cgtatcacct acgattccga acaggttgct tcttcctctt ccggcgcact
    12241 gattactgtt gtgcgtgaag gtgcgtcgat catcggcctg ttcatcatga tgttctatta
    12301 cagttggcaa ttgtcgatca ttttgattgt gctggcaccg attgtttcga ttgcgattcg
    12361 cgtagtatcg aagcgttttc gcaacatcag taaaaacatg cagaacacca tggggcaggt
    12421 gaccaccagc gctgaacaaa tgctgaaagg ccataaagaa gtattgattt tcggtggtca
    12481 ggaagtggaa acgaaacgct tcgataaagt cagcaaccga atgcgtcttc aggggatgaa
    12541 aatggtttca gcctcttcca tctctgatcc gatcattcag ctgatcgcct ctttggcgct
    12601 ggcgtttgtt ctgtatgcgg cgagcttccc aagtgtcatg gatagcctga ctgccggtac
    12661 gattaccgtt gttttctctt caatgattgc actgatgcgt ccgctgaaat cgctgactaa
    12721 cgttaacgcc cagttccagc gcggtatggc ggcttgtcag acgctgttta ccattctgga
    12781 cagtgagcag gagaaagacg aaggtaagcg cgtgatcgag cgtgcgactg gcgacgtgga
    12841 attccgcaac gtcaccttta cttatccggg acgtgacgta cccgcattgc gtaacatcaa
    12901 cctgaaaatt ccggcaggga agacggttgc tctggttgga cgctctggtt caggtaaatc
    12961 aaccatcgcc agcctgatca cgcgttttta cgatattgat gaaggcgaaa tcctgatgga
    13021 tggtcacgat ctgcgcgagt ataccctggc gtcgttacgt aaccaggttg ctctggtgtc
    13081 gcagaatgtc catctgttta acgatacggt tgctaacaac attgcttacg cacggactga
    13141 acagtacagc cgtgagcaaa ttgaagaagc ggcgcgtatg gcctacgcca tggacttcat
    13201 caataagatg gataacggtc tcgatacagt gattggtgaa aacggcgtgc tgctctctgg
    13261 cggtcagcgt cagcgtattg ctatcgctcg agccttgctg cgtgatagcc cgattctgat
    13321 tctggatgaa gctacctcgg ctctggatac cgaatccgaa cgtgcgattc aggcggcact
    13381 ggatgagttg cagaaaaacc gtacctctct ggtgattgcc caccgcttgt ctaccattga
    13441 aaaggcagac gaaatcgtgg tcgttgagga tggtgtcatt gtggaacgcg gtacgcataa
    13501 cgatttgctt gagcaccgcg gcgtttacgc gcaacttcac aaaatgcagt ttggccaatg
    13561 atcgaaaaaa tctggtctgg tgaatcccct ttgtggcggc tattgctgcc actctcctgg
    13621 ttgtatggcc tggtgagtgg cgcgatccgt ctttgctata aactaaaact gaagcgcgcc
    13681 tggcgtgccc ccgtaccggt tgtcgtggtt ggtaatctca ccgcaggcgg caacggaaaa
    13741 accccggtcg ttgtctggct ggtggaacag ttgcaacagc gcggtattcg cgtgggggtc
    13801 gtgtcccggg gatatggtgg taaggctgaa tcttatccgc tgttattgtc ggcagatacc
    13861 accacagcac aggcgggtga tgaacctgtg ttgatttatc aacgcactgg cgcgcccgtt
    13921 gcggtttctc ccgtgcgttc tgatgcggta aaagccattc tggcgcaaca ccctgatgtg
    13981 cagatcatcg taaccgacga cggtttacag cattaccgtc tggcgcgtga tgtggaaatt
    14041 gtcgttgttg atggtgtgcg tcgctttggc aatggctggt ggttgccggc ggggccaatg
    14101 cgtgagcgag cggggcgctt aaagtcagtt gatgcggtaa tcgtcaacgg cggtgtcccc
    14161 cgcagcggtg aaatccccat gcatctgctg ccgggtcagg cggtgaattt acgtaccggt
    14221 acgcgttgtg acgttgctca gcttgaacat gtggtggcga tggcagggat tgggcatccg
    14281 ccgcgctttt ttgccacgct gaagatgtgc ggcgtacaac cggaaaaatg tgtaccgctg
    14341 gccgatcatc agtctttgaa ccatgcggat gtcagcgcgt tggtaagcac cgggcaaacg
    14401 ctggtaatga ctgaaaaaga tgcggtgaaa tgccgggcct ttgcagaaga aaattggtgg
    14461 tatttgcccg ttgacgcaca gctttcaggt gatgaaccag cgaaactgct tgcgcaacta
    14521 acctcgctgg cttctggcaa ctagttacgc cgcggcagcg ttcgattgat ggagtcatga
    14581 atgtcgctgc cgcacctctc ccttgctgat gcgcgtaatc ttcaccttgc cgcgcaaggc
    14641 ctgttaaaca aaccccgccg tcgagcgtcg ttggaggata ttccggcaac gatctcccgc
    14701 atgtccttgc tgcaaatcga taccatcaat attgttgccc gtagcccata tctggtgctt
    14761 ttcagtcgtc tgggaaatta tcctgcccag tggctggatg agtctctggc gcgtggcgaa
    14821 ttaatggaat actgggcgca tgaagcctgc tttatgccac gcagcgactt tcgtcttatt
    14881 cgccaccgca tgctggcacc tgaaaaaatg ggctggaaat acaaagacgc ctggatgcag
    14941 gaacatgagg cggaaattgc acagttaatt cagcatattc atgataaggg gccggtacgt
    15001 tcagccgatt ttgagcatcc tcgtaaaggt gcaagcggct ggtgggaatg gaagccgcat
    15061 aaacggcatc tggaaggttt atttactgcc ggaaaggtga tggtgattga acggcgcaac
    15121 ttccagcgcg tttatgattt aacccaccgt gtcatgcctg actgggatga tgagcgcgat
    15181 ctcgtttcgc aaacagaagc agaaatcatc atgctggata acagtgcgcg tagcctggga
    15241 atattccgcg aacagtggct ggcagattac tatcggctga aacgtccggc actggcagcg
    15301 tggcgcgaag cgagggctga acagcggcaa atcattgctg tgcatgttga aaaattgggc
    15361 aatctttggc tgcatgctga tttgctgccg ctactcgagc gagcgctggc cggaaagctc
    15421 actgcaacgc acagcgcggt actttcgcct tttgatcctg ttgtctggga tcgcaaacgc
    15481 gcagagcagc tttttgattt tagctaccgg ctggagtgct ataccccagc gccgaaacgc
    15541 cagtatggct attttgttct gccgttatta catcgtgggc aattagttgg gcgaatggat
    15601 gccaaaatgc atcgccagac aggcatcctt gaagttatct ctctgtggtt acaggaaggt
    15661 attaaaccaa cgacaacgct gcaaaaaggg ttacgtcagg cgattactga tttcgctaac
    15721 tggcagcagg caacgcgggt gacattagga cactgcccgc aaggtctctt tacggattgc
    15781 cgcaccggct gggaaataga ccccgtcgca taaatgatta tgataagatt tatgcattca
    15841 taacccgatc catctggagg aactatggat catcgtctgc ttgaaatcat tgcctgcccg
    15901 gtttgcaacg gaaaactttg gtataaccag gaaaaacaag agcttatttg caaactggac
    15961 aacctggctt tcccgctgcg cgatggcatt ccagtgttgc tggaaacgga agcccgcgtg
    16021 ctgactgctg atgagagtaa atcatgagtt ttgtggtcat tattcccgcg cgctacgcgt
    16081 caacgcgtct gcccggtaaa ccattggttg atattaatgg caaacccatg attgttcatg
    16141 ttcttgaacg tgctcgcgaa tcaggtgccg agcgcatcat cgtggcaacc gatcatgagg
    16201 atgtcgcccg cgccgttgaa gccgctggcg gtgaagtatg tatgacgcgc gccgatcacc
    16261 agtcagggac agaacgcttg gcggaagttg tcgaaaaatg cgcgttcagc gacgacacgg
    16321 tgatcgttaa tgtgcagggt gatgaaccga tgatccctgc gacaatcatt cgtcaggttg
    16381 ctgataacct cgctcagcgt caggtgggta tggcgactct ggcggtgcca atccacaatg
    16441 cggaagaagc gtttaacccg aatgcggtga aagtggttct cgacgctgaa gggtatgcac
    16501 tgtacttctc acgcgctacc attccgtggg atcgtgatcg ttttgcaaaa ggccttgaaa
    16561 ccgttggcga taacttcctg cgtcatcttg gtatttatgg ctaccgtgca ggctttatcc
    16621 gtcgttacgt cacctggcag ccaagcccgt tagaacacat cgaaatgtta gagcagcttc
    16681 gtgttctgtg gtacggcgaa aaaatccacg ttgctgtcgc gcatgaagtt cctggcacag
    16741 gtgtggatac ccctgaagat ctcaagcgcg ttcgcgctga aatgcgctaa tttcacttca
    16801 cgacacttca gccaattttg ggaggagtgt cgtaccgtta cgattttcct caatttttct
    16861 tttcaacaat tgatctcatt caggtgacat cttttatatt ggcgctcatt atgaaagcag
    16921 tagcttttat gagggtaatc tgaatggaac agctgcgtgc cgaattaagc catttactgg
    16981 gcgaaaaact cagtcgtatt gagtgcgtca atgaaaaagc ggatacggcg ttgtgggctt
    17041 tgtatgacag ccagggaaac ccaatgccgt taatggcaag aagctttagt acgcccggaa
    17101 aagcccgaca actggcatgg aaaaccacca tgctggcaag aagtgggact gtccgtatgc
    17161 cgactattta tggtgtgatg acgcatgaag aacaccccgg ccctgatgtc ctgctactgg
    17221 agcggatgcg tggtgtttcg gtggaggcac cagcccgaac accagaacgt tgggaacagc
    17281 tcaaagacca aatcgttgaa gccttactgg cctggcaccg tcaggacagt cgcggttgcg
    17341 tcggcgcggt cgacaatact caggaaaatt tctggccctc atggtaccgg caacatgttg
    17401 aagtgctatg gaccacgctc aatcagttca ataacaccgg tctgacgatg caggataagc
    17461 ggatcctgtt tcgcactcgc gaatgtctcc cggcattatt tgaaggcttt aacgacaatt
    17521 gtgtgttgat tcacggtaac ttctgtttac gcagcatgct gaaagattcg cgcagcgatc
    17581 agttactggc gatggtcggg ccgggactaa tgctttgggc accgcgagaa tacgaactgt
    17641 tccgactaat ggataattct ctggcggaag atttgctctg gagttacctg caacgcgcgc
    17701 cagtggcgga gtcgttcatc tggcggcgtt ggttgtatgt gttatgggat gaagttgcgc
    17761 aactggttaa taccggacga tttagtcggc gcaacttcga tctggcatca aaatcactct
    17821 tgccgtggct cgcctgacgg acctttcagc cactgccaga tgcggccaag cgtttcatag
    17881 ccaacacgat cgctatgcat cagccagacc ggagaaggga ttgcccgttc ccacggatta
    17941 agcggcgagt cgatggccaa ctgattcgcc ggggctggta gcggatttaa cccttcctgc
    18001 tgaaaaaaga tcatcgcccg cggcagatgt gatgcggagg tcaccagcag gaaaggggcg
    18061 tcaccaatcg cctgtttcac tgccgcagct tcttcttcgg tatcttttgg caaatccagg
    18121 gtgataattt gctcgcgcgg tacgcccagc gattgcgcaa ctctggcacc tacttccgct
    18181 gtactcaccg tattggtttt tgctacgcct cccgtgaaga tcagttttga acccggatta
    18241 tcgcgccata aacggatacc ctcattcagg cgtggcagac tgttattgat taaattagag
    18301 ctcggtgccc actgcgggtt ccaggtataa ccacctccga gcaccacgat atagtccact
    18361 ttttgcgaat tattccatgt cggataggtg ctttcgatgg gacgcagcag gcggtcggct
    18421 accggttgca aactcaataa caagagtgac agccacccaa tgctaataaa aatcttcccg
    18481 gttttttgaa aacggctaaa ccacagcagc gccaggccag cgccaataat taacaacatc
    18541 agcggaagtg gcagcaacat attgccaatc acttttttca gtgtaaaaag catcctttct
    18601 ggttcctttt ttaaccatat agcaagggat cgccagcgat attacaccag acgggttcat
    18661 tctcgccgtg gctgtgacaa aatagcgtct ttatcagaaa acgtcttagc caaccggtgg
    18721 agaaagcaat gcaggatcgc aattttgatg atattgcgga aaagttttcc cgtaacattt
    18781 acggcaccac caaagggcag cttcgacagg ctattctgtg gcaggatctc gatcgcgtgc
    18841 tggcggaaat gggtccgcaa aaactgcgtg tgctggatgc gggcggtgga gaagggcaga
    18901 ccgcaatcaa aatggccgag cgcgggcatc aggtcatttt atgcgatctt tctgcgcaga
    18961 tgatcgaccg cgcaaaacag gcggcagaag caaaaggtgt gagcgacaac atgcaattta
    19021 tacattgcgc cgctcaggat gttgcttcgc atttggaaac gcccgttgat ctgatattgt
    19081 tccatgcggt gctcgagtgg gtggctgatc cccgcagcgt attgcagacc ctgtggtcag
    19141 tgttgcgtcc aggcggcgtg ttgtcgttaa tgttctacaa tgcgcatggt ttgttgatgc
    19201 ataacatggt cgccgggaat tttgattacg tgcaggcggg aatgccgaaa aagaaaaaac
    19261 ggacgctttc gccagattat ccacgcgacc cggcgcaggt ttatctgtgg ctggaagaag
    19321 ctggttggca aattatgggt aagacaggcg ttcgcgtgtt tcatgattat ctgcgcgaga
    19381 agcaccagca gcgcgactgc tatgaagcat tacttgaatt agaaacgcgt tattgccgtc
    19441 aggaaccgta tattaccctg gggcgttata ttcatgtcac cgcgcgcaaa ccgcagagca
    19501 aggataaagt atgagtgaat tttcccagac agtccccgaa ctggttgcct gggccagaaa
    19561 aaatgacttc tccatctcgc tgccggtaga ccgactctct tttctgctgg cggttgccac
    19621 gctgaacggc gagcgtctgg atggtgagat gagtgaaggc gagctggtgg atgcattccg
    19681 ccatgtgagt gatgcgtttg agcaaaccag cgaaaccatc ggcgtgcgcg ccaataacgc
    19741 gatcaacgac atggtgcgtc aacgtctgct gaaccgcttt accagcgagc aggcagaagg
    19801 gaacgcaatt taccgtctga cgccgctcgg catcggcatt actgactatt acatccgtca
    19861 gcgcgagttt tctacgctgc gcctttctat gcagttgtcg attgtggcgg gtgagctcaa
    19921 acgcgcagca gatgccgccg aagagggcgg tgatgaattt cactggcacc gtaatgtcta
    19981 tgcgccactg aaatattcgg tagcagaaat tttcgacagt atcgacctga cgcaacgtct
    20041 gatggacgaa cagcagcagc aggtgaagga cgatatcgcc cagttgctga acaaagactg
    20101 gcgggcggcg atttccagct gtgaattgtt gctttcggaa acttccggaa cgctgcgtga
    20161 attgcaggat acactggaag cggcaggcga caaattgcag gctaatctgt tgcgcattca
    20221 ggatgcgacg atgactcatg acgatctgca ttttgtcgat cgtctggtgt tcgatctgca
    20281 gagcaaactc gaccgtatta tcagttgggg ccagcaatcc atcgacttgt ggattggcta
    20341 cgaccgccac gtacacaaat ttattcgtac cgcgatcgat atggataaaa accgcgtctt
    20401 tgctcagcgg ttacgtcagt cggtacaaac ctattttgat gagccgtggg cgctaactta
    20461 tgccaatgcc gatcgtctgc tggatatgcg tgacgaagag atggcactgc gcgatgaaga
    20521 agtgactggg gaacttcctg aggatctgga atacgaagag tttaacgaga tccgcgaaca
    20581 gctggcggcg atcatcgaag aacaacttgc tgtgtacaaa accagacaag tgccgctgga
    20641 tcttggtctg gtggtacgcg aatatctgtc acagtatccg cgtgcacgtc actttgacgt
    20701 tgcgcgcatt gttattgatc aggcggtacg tcttggcgta gcgcaagcag atttcaccgg
    20761 actgccagcg aaatggcagc cgattaatga ttacggagcc aaggtacagg cgcatgtcat
    20821 cgacaaatat tgaacaagtg atgccggtta agctggcgca ggcgctggcg aatccgttat
    20881 ttccggcgct ggacagcgcc ttacgttcag gccgccatat tggcctcgac gaactggata
    20941 atcatgcatt cctgatggat tttcaggaat atctggaaga gttttacgcg cgttataacg
    21001 ttgagcttat tcgtgcaccg gaagggttct tctatttacg tccacgttcc accacgctga
    21061 tccctcgttc cgtcttgtcg gaactggata tgatggtcgg gaaaatcctc tgctatctct
    21121 atctcagccc ggaacggctg gcgaatgagg ggatttttac tcaacaggaa ctgtacgacg
    21181 aactgctcac cctggccgat gaagcgaaac tgctgaaact ggtgaacaac cgttcaaccg
    21241 gttcagacgt tgaccgtcag aagttgcagg aaaaagtacg ttcttcgctc aaccgtctgc
    21301 gtcgtttagg catggtgtgg tttatgggcc acgacagcag caagttccgc attaccgaat
    21361 cggtgttccg cttcggagcc gatgtgcgtg ctggcgacga tccccgtgaa gcacagcgtc
    21421 gcctgattcg tgatggcgaa gcaatgccga ttgaaaatca tctgcaactc aacgatgaaa
    21481 ccgaagagaa tcagccagat agcggagagg aagaataatg attgaacgcg gtaaatttcg
    21541 ctcactgacg ctgattaact ggaacggctt ttttgcccga acttttgacc ttgacgagct
    21601 ggtcacgacg ctttctggcg gtaacggggc gggtaaatcc accaccatgg cggcgttcgt
    21661 tacggcgctg atccccgacc tgaccctgct gcatttccgt aacactacgg aagccggggc
    21721 caccagcggt tcgcgcgata aaggtctgca cggtaagctg aaagcgggtg tctgttattc
    21781 gatgctcgac accattaact cgcgccacca gcgcgtggtg gtcggtgtgc gtctgcaaca
    21841 ggttgccgga cgcgatcgta aagtggatat caaaccattt gccattcagg gactgccgat
    21901 gtcggtgcag ccgacacagc tggtgaccga aactctgaac gaacgccagg cgcgcgtgct
    21961 gccgcttaac gagctgaaag acaagctcga ggcgatggaa ggcgtgcagt ttaaacagtt
    22021 caactccatt accgattacc actcgctgat gttcgatctg ggcatcatcg cgcgtcgtct
    22081 gcgctccgca tctgaccgta gcaaattcta tcgtctgatc gaagcttcgc tgtatggcgg
    22141 gatctccagt gccattaccc gttctctgcg cgactacctg ttgccggaaa acagcggcgt
    22201 gcgtaaagcg ttccaggaca tggaagcggc gctgcgtgaa aaccgcatga cgctggaagc
    22261 gattcgtgtc acccaatcgg accgtgacct gtttaagcat ctgatcagcg aagctaccaa
    22321 ctacgtggcg gcggactaca tgcgtcacgc caacgagcgc cgtgtccatc tcgacaaagc
    22381 gctggagttt cgtcgcgagc tacatacttc gcgtcagcaa ctggcggctg agcagtacaa
    22441 acacgtcgat atggcgcgtg agctggcaga gcacaacggt gccgaaggcg atctggaagc
    22501 ggattatcag gcggccagcg atcacctgaa cctggtgcaa accgcactgc gtcagcagga
    22561 gaagatcgaa cgctacgaag cggatctcga tgagctgcag atccgtctgg aagagcaaaa
    22621 cgaagtggtg gcagaagcca tcgaacgcca ggaagagaac gaggctcgtg cggaagctgc
    22681 cgaactggaa gtggacgagc tgaaaagcca gcttgctgac taccagcagg cgctggacgt
    22741 ccagcaaacg cgcgcgatcc agtataacca ggcgattgct gcgcttaatc gtgccaaaga
    22801 actgtgccat ctgccggact taaccgccga cagcgccgcc gaatggctgg aaaccttcca
    22861 ggcgaaagag ctggaagcga ctgaaaaaat gctctctctt gagcagaaaa tgagcatggc
    22921 gcaaaccgcg cacagccagt ttgagcaggc ttatcagctg gtggtggcaa tcaacggccc
    22981 gctggcgcgt aacgaggcgt gggatgtcgc ccgcgaactg ctgcgcgaag gggtcgagca
    23041 gcgtcacctg gcagatcagg ttcaaccgtt gcggatgcgc ttaagtgaac tggaacagcg
    23101 tctgcgcgag cagcaagaag ccgaacgcct gctggcagat ttctgcaaac gtcagggcaa
    23161 gaattttgat atcgacgaac tggaagccct gcatcaggag ctggaagcgc gtattgcctc
    23221 tctttccgat agcgtgtcta acgcccgcga agagcgtatg gcactgcgcc aggagcagga
    23281 acagctgcag tctcgcattc agagtttgat gcagcgtgca ccggtttggc tggcagcgca
    23341 aaacagtctc aaccagttga gtgaacagtg cggcgaagag ttctcctcca gccaggacgt
    23401 cactgaatat ctgcaacagt tgctggagcg tgagcgcgag gcgattgttg aacgcgatga
    23461 agtaggcgcg cgcaaaaacg ccgtcgatga agagatcgaa cgtttaagcc agcctggcgg
    23521 ctctgaagat cagcgtctga acgcgctggc ggagcgtttt ggtggtgtgc tgctgtcaga
    23581 aatttatgat gacgttagcc tggaagatgc gccgtacttc tcagcgctgt atggcccgtc
    23641 acgccacgcc atcgtggtgc cagatctatc acaggtaact gaacacctgg agggcttgac
    23701 cgattgcccg gaagatctct atttgatcga aggggatccg cagtcattcg atgacagcgt
    23761 gttcagcgtt gatgagctgg aaaaagcggt agtggtgaaa atcgccgatc gtcagtggcg
    23821 ttattcacgt ttcccggaag tgccgctgtt tggtcgtgct gcgcgtgaaa gccgtattga
    23881 aagcctccat gccgagcgtg aagtgctttc cgaacgcttc gccacgctct cctttgacgt
    23941 acagaaaacc cagcgtctgc atcaggcgtt cagccgcttt atcggtagtc atctggcggt
    24001 ggcgtttgag tctgacccgg aagcagaaat ccgtcaactg aacagccgtc gcgtcgaact
    24061 ggagcgggcg ttgagtaatc atgaaaatga taaccagcag cagcgtattc agtttgagca
    24121 ggcgaaagaa ggcgttacgg cgctgaaccg cattctgccg cgtctcaacc tgttggctga
    24181 tgacagcctg gcggatcgcg tcgatgaaat ccgcgaacgt ctggatgaag cccaggaagc
    24241 cgcgcgtttt gttcagcagt ttggcaatca actggcgaaa ctggaaccga tcgtttcggt
    24301 attgcagagc gacccggaac agttcgaaca gttaaaagaa gattacgcgt actctcagca
    24361 gatgcagcgc gatgcccgtc agcaggcgtt tgccctgacg gaagtggtgc agcgtcgtgc
    24421 gcactttagc tattctgact cggcagaaat gcttagcggt aacaacgatc tcaacgaaaa
    24481 actgcgtgaa cgtctggaac aggcggaagc ggagcgtacc cgcgctcgcg aagcgttgcg
    24541 cggtcacgca gcgcagttga gtcagtacaa ccaggtgctg gcttcgctga aaagttctta
    24601 cgacaccaaa aaagagctac tcaacgatct gcaacgtgaa ttgcaggata tcggcgtgcg
    24661 tgctgatagc ggggcagaag agcgggcacg tattcgccgt gacgagctgc atgcgcaact
    24721 gagcaataac cgttcgcgcc gcaaccaact ggaaaaagcg ctcaccttct gcgaagctga
    24781 gatggacaac ctgacccgca aactgcgcaa gctggagcgt gattactttg agatgcgcga
    24841 gcaggtagtg accgcgaaag cgggctggtg tgcagtcatg cgcatggtga aagataacgg
    24901 cgttgagcgc cgcttacacc gtcgtgagct ggcttatctc tccgctgatg atttgcgttc
    24961 catgtcggat aaggcgttag gtgcgctgcg tctggcggtg gcggataacg aacatctgcg
    25021 cgacgtgctg cgcatgtcgg aagatccgaa acgtccggag cgtaaaattc agttcttcgt
    25081 ggcggtttat cagcatctgc gtgaacgtat tcgtcaggat attattcgta ccgatgatcc
    25141 ggtggaagct atcgaacaga tggagatcga acttagccgt ctgaccgaag agttaacctc
    25201 ccgtgaacag aaactggcga tcagttcccg cagcgtggcg aacatcattc gcaaaaccat
    25261 tcagcgcgaa cagaatcgta tccgtatgct caaccagggg ttgcagaacg tatcgtttgg
    25321 tcaggtgaac agcgtacgtc tgaacgtgaa cgtgcgtgaa acgcacgcca tgctgttgga
    25381 tgtgctctct gaacagcacg agcagcatca ggatctgttt aacagcaacc gtctgacctt
    25441 ctcggaagcg ctggcgaaat tgtatcagcg tcttaatccg cagattgata tggggcagcg
    25501 cacgccgcag accattggcg aagaactgct ggattaccgc aactatctgg aaatggaagt
    25561 tgaggttaac cgtggttccg atggctggtt gcgcgcagag tctggtgcat tgtcgaccgg
    25621 tgaggcgatt ggtaccggta tgtcgattct ggtgatggtg gtacaaagct gggaagatga
    25681 atctcgccgc ctgcgcggta aagatatctc tccttgccgc ctgctgttcc tcgatgaagc
    25741 agcgcgactg gatgctcgtt ctatcgccac gctgtttgaa ttgtgtgaac gtttgcaaat
    25801 gcagctcatc atcgcagcgc cggaaaatat cagcccggag aaaggcacca cctataaact
    25861 ggtgcgtaaa gtcttccaga ataccgaaca cgttcacgtc gtcggcctgc gaggatttgc
    25921 gccgcaactc cctgaaacgc ttccaggaag tgacgaagcg ccttctcagg cgagttaaaa
    25981 ttaaggcggc agcaatgccg ccttttcttt ttccgaaaac tccgtttctg cactaaaaaa
    26041 gtggcacatt acggcgcgct tttctttaaa cttctttaca ttaggttatg taaaaacgta
    26101 tcggcgttta tatactgaag ataagcctga tgagtaacag gcttgctcgt catactttcg
    26161 tgagtattgg cgttgtacag gcaagtcgta aaataacagc ctggctattc agagtatgat
    26221 aaaaacaggg ggcaagggat gttgcttaat atgatgtgtg gtcgtcggct gtcggcaatc
    26281 agtttgtgcc tggccgtaac attcgctcca ctgttcaatg cgcaggccga tgagcctgaa
    26341 gtaatccctg gcgacagccc ggtggctgtc agtgaacagg gcgaggcact gccgcaggcg
    26401 caagccacgg caataatggc ggggatcctg ccattgcctg aaggtgcggc agaaaaagcc
    26461 cgcacgcaaa tcgaatctca attacccgca ggttacaagc cggtttatct taaccagctt
    26521 caactgttgt atgccgcacg cgatatgcaa cccatgtggg aaaaccgtga tgctgttaaa
    26581 gccttccagc aacagctggc agaggtggcg attgccggtt tccagccgca gtttaataaa
    26641 tgggtagagt tactgaccga tcctggtgtt aacgggatgg cacgcgacgt ggtgctctct
    26701 gatgcgatga tgggctatct ccatttcatt gcaaatattc cggtcaaagg cactcgctgg
    26761 ctatatagca gtaaacctta tgcgctttca acgccgccgc tttcggtgat taaccaatgg
    26821 cagcaggcgc tggataaagg tcaattgcct acgtttgttg caggactggc accgcagcat
    26881 ccgcaatatg cggcgatgca tgaatcgtta ctggccttac tcagtgacac caaaccgtgg
    26941 ccccaactga ccggcaaagc aacgttgcgc ccagggcagt ggagtaacga cgtaccggcg
    27001 ttgcgcgaaa tattgcaacg cacaggcatg ttggacgggg ggccgaaaat tactctacct
    27061 ggcgatgaca cgccaactga cgcggtagtc agcccatccg ctgttactgt tgaaacagca
    27121 gaaactaagc cgatggacaa gcaaacgacg tctcgtagta aacctgcgcc tgccgttcgt
    27181 gccgcctacg ataatgaact ggtggaagcc gttaaacgtt ttcaggcatg gcaaggattg
    27241 ggggcagatg gtgctattgg cccggcaacg cgggactggt taaacgtaac gcccgcccag
    27301 cgtgctggcg tgttggcact caacatccag cgattgcgct tgctgccaac agagctttct
    27361 accgggatca tggttaacat tccggcctat tcgctggtct actatcagaa cggcaatcag
    27421 gtgctggatt cgcgagtcat tgtcggtcgc cccgatcgca aaacgccgat gatgagcagt
    27481 gcacttaaca acgtagtggt aaacccgccg tggaacgtac caccaactct ggcacgcaaa
    27541 gatattctgc cgaaagtgcg caacgatccg ggatatctcg aaagccatgg ctatacggtg
    27601 atgcgcggct ggaacagcag agaagcgatt gacccatggc aggttgactg gtctacaatc
    27661 acggcctcga atttaccgtt tcgcttccag caggctccag gcccacggaa ctcgctgggg
    27721 cgctataaat tcaatatgcc gagttcagag gccatttatt tgcatgacac gccgaaccac
    27781 aatctgttca agcgtgatac acgcgcattg agctcaggct gtgtacgagt gaataaagct
    27841 tccgatctgg cgaatatgct gttgcaggat gcaggctgga atgacaaacg tatttctgat
    27901 gcgctgaagc agggtgatac acgttacgtc aatattcggc agtcgattcc ggtgaatctc
    27961 tactacctga cggcctttgt tggtgcagat ggtcgtaccc agtatcgtac agatatttac
    28021 aattatgatc tgcctgcgcg atccagctcg caaatcgtat cgaaagcgga acaattaatc
    28081 aggtaaatga agtagttcgg ggaaatcagt tgtcgtaata attagcatga ttgggggtgg
    28141 ttctctgcag cccccgtcac tgctggggtt gagtcatctt gacgtctgct ttacgggcgg
    28201 ttaaggtgcc tcttgtgcgc cagaagtgca tataaacgat aacattgacc tgtagacttg
    28261 attatcatgg acaaattcga cgctaatcgc cgcaaattgc tggcgcttgg tggcgttgca
    28321 ctcggtgccg ccatcctgcc gacccctgcg tttgcaacac tctctacccc acgcccgcgc
    28381 attttgacac tcaataatct tcataccgga gagtcaatca aagcggagtt tttcgatggc
    28441 agaggctata ttcaggaaga attggcaaaa cttaaccatt ttttccgcga ttaccgcgcg
    28501 aacaaaataa agtccatcga cccaggatta ttcgaccagt tgtatcgcct gcaagggtta
    28561 ttaggcaccc gcaaaccggt gcaactcatt tccggttatc gttccattga taccaacaat
    28621 gaactacgcg cccgcagccg tggagtagcg aagaaaagct atcacactaa aggccaggcg
    28681 atggatttcc atattgaagg tatcgcgtta agcaatattc gcaaagccgc gttatctatg
    28741 cgcgcaggtg gtgtaggata ttacccacgt agtaactttg tgcatattga taccgggcca
    28801 gcacggcact ggtagtaatt gcttaacgaa acaggggcag tatgaactat cgtattattc
    28861 cggtcaccgc attctcccag aactgttcat tgatctggtg tgaacaaacc cgactggccg
    28921 cactggtcga tcctggcggc gatgcggaaa aaatcaaaca ggaagttgat gccagcggcc
    28981 tgacactgat gcagatcctg ctgacgcatg gtcatctgga ccacgttggc gcagcggcgg
    29041 aactggcgca acattacggc gtgccggttt ttggcccgga aaaagaagat gagttctggc
    29101 tgcaaggctt gcctgcgcaa agtcgtatgt ttggtctgga agagtgtcag ccgctgacgc
    29161 cagatcgttg gctgaaagaa ggcgatacca tcagcatagg gaatgtgact ttacaggtgt
    29221 tacattgccc tgggcatacg ccgggtcatg tcgtgttttt tgatgatcgg gcaaagctgc
    29281 tgatttctgg cgatgttatt ttcaaaggcg gagtagggcg cagtgacttc ccgcgtggcg
    29341 atcataatca actgatttct tcaatcaaag ataaattgct gccactgggg gatgacgtga
    29401 catttattcc gggtcacgga ccattatcca cacttggtta tgaacgcctg cataatccct
    29461 tcctgcaaga cgaaatgccc gtctggtaag gcacacataa aaaagcccgc ttttcagcgg
    29521 gcttcattgt ttttaacgct tacagcactg ccacaatcgc ttcgcacagc ggagccatgt
    29581 tatctggtgt catccctgcc acatttacgc gaccagaagc aacagcatat acgccaaact
    29641 cttcgcgcag acgcaacact tgttcttttg tcagaccact gaaggagaac atgccgttct
    29701 gtttgatgat aaagctgaag tcgcggtttg cccctttttc ctgcagcgta ttgacgaaca
    29761 actgacgcat acgctgaata cgctggcgca tatcagtcag ctcttgttcc caaatcgcac
    29821 gtaatgcatc gttgctcagg atggtggcaa caacagaagc gccgtgtgct ggagggttag
    29881 agtagttagc gcgaatcgcc gctttcattt ggctgaatgc gcgatcaacg gtttcactgt
    29941 cggcagcaac cagagtacaa gcgccaacac gctcgttgta caggccaaag tttttagagt
    30001 aggaactggc aacaatcagc tctttatgca tagccgcgaa agcgcgcagt ccttcagcat
    30061 cttcttccag accacgggca aaaccctggt aagcgaagtc aaacagcggt aaccagcctt
    30121 tctcaacgga gagttgtgcc agtgtctgcc attgttccag cgtagggtcg ataccggttg
    30181 ggttatggca gcagccatgg aacagcacta cgtcgccagc ctgagcttcg ttcaggctgt
    30241 taatcagtgc atcgaagtca agggtgtgat tttccgcatc ataataagcg tattcacgaa
    30301 cttccagacc tgcagagtta aagacgctct tatggttcgg ccagcttggg ttgctcaccc
    30361 acacacgctt aacgctggta ttttttgcca ggaagtcggc agccacgcgt agtgcgccag
    30421 tgccacccgg agtctgtgcc gtgcgagcac gtttgtcatt gatcagggcg ctacctttac
    30481 caaacagcag ttcctgagtg cagcgaccaa attcagggat gccgtcaatg ccgaggtaat
    30541 ttttggtggt ttcattttcg agcagatact gttcagcctt tttcacgctg gtcagtaccg
    30601 gggttttgcc cgtctcatct ttatagacac caatcccgag gttaattttg ccgggacgtt
    30661 catcggcacg aaacagatcg gccaggccca gaatcgggtc ggcaggagcg gcggtaatgt
    30721 tctcaaacat gacgaggttc cattatggtt acagaaggga agtccgctat cagggtaacg
    30781 ggagatttac aaaattccaa ctattactga tgaaaacgca ggctgttttt gcaagacgtg
    30841 agattgctct ggaaggcata aaaaaaacag gaccaaagtc ctgttttttc ggcatttaac
    30901 aaagaggtgt gctattagaa ctggtaaacg atgcccacag caacggtgtc gtctgaacct
    30961 acgcccagtt tgttgtcaga atcgatctgg ttgatgatgt agtcaacata ggtggacatg
    31021 tttttgttga agtagtaggt tgcgcccact tcaaagtagt tcaccagatc aacatcaccg
    31081 ataccttcta cgtctttcgc tttagatttg gtgtaagcga tggacggacg cagaccgaaa
    31141 tcgaactggt attgcgcaac taacagaacg tcttgcgttt tgttggcgaa gccgccttca
    31201 attggcgtag cgttacgggt ttcaccgtag ttcgctgcca ggtagatgtt gttcgcgtca
    31261 tacttcagac cagttgccca ctgttcagct tttttaccct gaccgcggaa ttcggcttcc
    31321 tgggcgtcgg tacggtctgc tgcaccgtaa gcacctacga taccaaagcc ttcgaattcg
    31381 tagctgatag agccaccgac gccgtcgccg ttggaacgct caggaatacc agcacgttcg
    31441 tttttgccca ggtactgaac ggcaaagttc aggccgtcaa ccagaccgaa gaagttggag
    31501 ttacgatagg tagcaacgcc gcctacacga ccaacgaaga agtcatcgct gtatgccgtg
    31561 tcaccgccaa actcaggcag catatcggtg taacccagtg cgtcgtaaac cacaccgtag
    31621 ttacgaccgt agtcgataga acccgcatca gcgaatttca gacctgcgaa tgccaaacga
    31681 gttttgttac ctttctgggc atcagcgcct tcagagttgt taccctggaa gttatattcc
    31741 cactgaccat aaccggtcag atcggaattg atttgtgttt cccctttaaa accaagacgg
    31801 gcataagttt tgtcgccgtt tccgccgtaa ctgtttacac cattgtcttt agaaaaataa
    31861 tgcagaccga cagctttacc gtacagatct actttgttgc catctttgtt atagatttct
    31921 gcagcgtttg cagtacctgc tactaacaga gcagggacga tcactgccag aatattgcgc
    31981 ttcatcatta tttattaccc tcatggtttt tttatgacac ctgccactgc cgtcaataag
    32041 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
    32101 ggcatctttc cattcaagct aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
    32161 tggtttcaaa aagaaaaaat atgtaactaa aagtaaaaa
//
LOCUS       DAZFKF010000051        32145 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFKF010000051 DAZFKF010000000
VERSION     DAZFKF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.051"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..98)
                     /locus_tag="ACHKA1_002824"
     CDS             complement(<1..98)
                     /locus_tag="ACHKA1_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680449.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRRRYIFS"
     gene            complement(157..1047)
                     /gene="rarD"
                     /locus_tag="ACHKA1_002825"
     CDS             complement(157..1047)
                     /gene="rarD"
                     /locus_tag="ACHKA1_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO5680450.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(1099..1566)
                     /gene="yigI"
                     /locus_tag="ACHKA1_002826"
     CDS             complement(1099..1566)
                     /gene="yigI"
                     /locus_tag="ACHKA1_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO5680451.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1731..2600
                     /gene="pldA"
                     /locus_tag="ACHKA1_002827"
     CDS             1731..2600
                     /gene="pldA"
                     /locus_tag="ACHKA1_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO5680452.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2733..4562
                     /gene="recQ"
                     /locus_tag="ACHKA1_002828"
     CDS             2733..4562
                     /gene="recQ"
                     /locus_tag="ACHKA1_002828"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO5680453.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4626..5246
                     /gene="rhtC"
                     /locus_tag="ACHKA1_002829"
     CDS             4626..5246
                     /gene="rhtC"
                     /locus_tag="ACHKA1_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO5680454.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5308..5928)
                     /gene="rhtB"
                     /locus_tag="ACHKA1_002830"
     CDS             complement(5308..5928)
                     /gene="rhtB"
                     /locus_tag="ACHKA1_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO5680455.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6039..7061
                     /gene="pldB"
                     /locus_tag="ACHKA1_002831"
     CDS             6039..7061
                     /gene="pldB"
                     /locus_tag="ACHKA1_002831"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO5680456.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7069..7869
                     /gene="yigL"
                     /locus_tag="ACHKA1_002832"
     CDS             7069..7869
                     /gene="yigL"
                     /locus_tag="ACHKA1_002832"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO5680457.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7945..8844
                     /gene="bioP"
                     /locus_tag="ACHKA1_002833"
     CDS             7945..8844
                     /gene="bioP"
                     /locus_tag="ACHKA1_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO5680458.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8732..9685)
                     /gene="metR"
                     /locus_tag="ACHKA1_002834"
     CDS             complement(8732..9685)
                     /gene="metR"
                     /locus_tag="ACHKA1_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO5680459.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9921..12182
                     /gene="metE"
                     /locus_tag="ACHKA1_002835"
     CDS             9921..12182
                     /gene="metE"
                     /locus_tag="ACHKA1_002835"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO5680460.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAQRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12222..13037)
                     /gene="ysgA"
                     /locus_tag="ACHKA1_002836"
     CDS             complement(12222..13037)
                     /gene="ysgA"
                     /locus_tag="ACHKA1_002836"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO5680461.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGSQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13299..14060
                     /gene="udp"
                     /locus_tag="ACHKA1_002837"
     CDS             13299..14060
                     /gene="udp"
                     /locus_tag="ACHKA1_002837"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO5680462.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14201..15628
                     /gene="rmuC"
                     /locus_tag="ACHKA1_002838"
     CDS             14201..15628
                     /gene="rmuC"
                     /locus_tag="ACHKA1_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO5680463.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINSDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15723..16478
                     /gene="ubiE"
                     /locus_tag="ACHKA1_002839"
     CDS             15723..16478
                     /gene="ubiE"
                     /locus_tag="ACHKA1_002839"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO5680464.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16492..17097
                     /gene="ubiJ"
                     /locus_tag="ACHKA1_002840"
     CDS             16492..17097
                     /gene="ubiJ"
                     /locus_tag="ACHKA1_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO5680465.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17094..18734
                     /gene="ubiB"
                     /locus_tag="ACHKA1_002841"
     CDS             17094..18734
                     /gene="ubiB"
                     /locus_tag="ACHKA1_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO5680466.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18813..19082
                     /gene="tatA"
                     /locus_tag="ACHKA1_002842"
     CDS             18813..19082
                     /gene="tatA"
                     /locus_tag="ACHKA1_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO5680467.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19086..19601
                     /gene="tatB"
                     /locus_tag="ACHKA1_002843"
     CDS             19086..19601
                     /gene="tatB"
                     /locus_tag="ACHKA1_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO5680468.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19604..20380
                     /gene="tatC"
                     /locus_tag="ACHKA1_002844"
     CDS             19604..20380
                     /gene="tatC"
                     /locus_tag="ACHKA1_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO5680469.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20422..21204
                     /gene="tatD"
                     /locus_tag="ACHKA1_002845"
     CDS             20422..21204
                     /gene="tatD"
                     /locus_tag="ACHKA1_002845"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO5680470.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21201..21689)
                     /gene="rfaH"
                     /locus_tag="ACHKA1_002846"
     CDS             complement(21201..21689)
                     /gene="rfaH"
                     /locus_tag="ACHKA1_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO5680471.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21856..23349
                     /gene="ubiD"
                     /locus_tag="ACHKA1_002847"
     CDS             21856..23349
                     /gene="ubiD"
                     /locus_tag="ACHKA1_002847"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO5680472.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23395..24096
                     /gene="fre"
                     /locus_tag="ACHKA1_002848"
     CDS             23395..24096
                     /gene="fre"
                     /locus_tag="ACHKA1_002848"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO5680473.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24288..25451)
                     /gene="fadA"
                     /locus_tag="ACHKA1_002849"
     CDS             complement(24288..25451)
                     /gene="fadA"
                     /locus_tag="ACHKA1_002849"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO5680474.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25461..27650)
                     /gene="fadB"
                     /locus_tag="ACHKA1_002850"
     CDS             complement(25461..27650)
                     /gene="fadB"
                     /locus_tag="ACHKA1_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO5680475.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVDDLLVEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27840..29171
                     /gene="pepQ"
                     /locus_tag="ACHKA1_002851"
     CDS             27840..29171
                     /gene="pepQ"
                     /locus_tag="ACHKA1_002851"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO5680476.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVED
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29171..29785
                     /gene="yigZ"
                     /locus_tag="ACHKA1_002852"
     CDS             29171..29785
                     /gene="yigZ"
                     /locus_tag="ACHKA1_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO5680477.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29824..31275
                     /gene="trkH"
                     /locus_tag="ACHKA1_002853"
     CDS             29824..31275
                     /gene="trkH"
                     /locus_tag="ACHKA1_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO5680478.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFRQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31287..31832
                     /gene="hemG"
                     /locus_tag="ACHKA1_002854"
     CDS             31287..31832
                     /gene="hemG"
                     /locus_tag="ACHKA1_002854"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO5680479.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7462 a         8185 c         8806 g         7692 t
ORIGIN      
        1 gaaaatatat atcggcgtct ggaaatctta ccgttagatg ttgggataaa catacgtaac
       61 ataaatttta catccttgtg tgagtctcct gtcagcatgg caatatgccc actctcatac
      121 aagagtcggc atattttttc agaatatatt tattttttat ttggacgttc tacgctgcgt
      181 ataaatcgca tccatcacaa aaattgccag cgccacccaa ataaaggcga aagtcaccat
      241 cttatcggca cccggttttt caccataaaa cgtcacagcc agcaggaaca tcagcgtcgg
      301 gccaatgtac tggaaaaagc ctaacgttga gagacgcagg cgcgtggcag cggcagtaaa
      361 acacaacagc ggtacggtag tgacaatacc ggcggcgatc agcagtaaat tcagcgacat
      421 cgggttttgc cccatatggc tggttgagct gtcggcaata gcaaacaggt aaatcgccgc
      481 cacgggcagc agccacatgg tttcaattaa catgccggtt tgtgcttcta ccgcaatctt
      541 cttgcgcacc aggccgtaga aggcaaaact aaatgccagc cccagcgcga taataggtag
      601 cgaaccaaaa gtccacagct ggactaacac gccacatatc gccagaatca ccgccagcca
      661 ttgcatccgg cggaatcgct cgccgaggaa aatcatcccc agcacaatgt tcaccagcgg
      721 gttaataaag taaccaaggc tcgcttccag catatggtga ttgttcaccg cccagataaa
      781 cagtagccag ttgccaccaa tcagtacggc agagactgcc agcataaaaa ttttctgtgg
      841 cgtctgaatc agcgttttta aataggacca ctggcggcaa atgctcatca gcaccaccat
      901 aaagaaaaac gaccagatca cgcgatgcgt caggatttca tcggcgggca cgtagtaaat
      961 caacttgaag tacgctgggg ctatacccca aataaaataa gcggcaagag cgagtaatac
     1021 gccctgccgc gtttgttttg catccatcgg gaatactcat ttttaattgg taacagcagt
     1081 ttacctgctt ttatgtcttc aacctaccat ataggtggcg gtggcactgg caatataaag
     1141 ctgttcttca ttgtgtaatt caacgcgggc gacggcgact ttattgcctg cacgcaacag
     1201 gctactggta gcagtaaaac gctcgcccct gcctgggcgc agataatcaa cgcgaagatc
     1261 aatggtcccc atccgcgata gccgctggcg tagttcatct tcactgatgg tttcgtggcg
     1321 ggttaaggta cttcccacgc acaccagacc ggcggcgaca tccagcgccg acgcaatgac
     1381 cccgccgtgc aaaatgcttt gcgcccagtt gcccaccatc attggctgat ttttaaaggc
     1441 cagctgtgcg aactcttttt cgtaacgctc cagttccatc cccaatgcgc ggttaaatgg
     1501 catgtgataa acaaacatct cacccactaa tttcagggct tgttcagcgg tcagtacggc
     1561 agacatatca tccttacact tcattggtta atgaaatgtt gattttatgc ttctttgttg
     1621 ttggtttcta ctttaggaag ggataactaa cggcaatgga gttaagtatg taaaatagcc
     1681 cgcagaaaaa cattcacctt atcaataatt cgttacggag aacacgaccg atgcggactc
     1741 tgcagggctg gttgttgccg gtgtttatgt tgcctatggc agtatatgca caagaggcaa
     1801 cggtgaaaga ggtgcatgac gcgccagcgg tgcgtggcag tattatcgcc aatatgctgc
     1861 aggagcatga caatccgttc acgctctatc cttatgacac caactacctc atctacaccc
     1921 aaaccagcga tctgaacaaa gaagcgattg ccagttacga ctgggcggaa aatgcgcgta
     1981 aggatgaagt aaagtttcag ttgagcctgg catttccgct gtggcgtggg attttaggcc
     2041 cgaactcggt gttgggtgcg tcttatacgc aaaaatcctg gtggcaactg tccaatagcg
     2101 aagagtcttc accgtttcgt gaaaccaact acgaaccgca attgttcctc ggttttgcca
     2161 ccgattaccg ttttgcaggt tggacgctgc gcgatgtaga gatggggtat aaccacgact
     2221 ctaacgggcg ttcagacccg acctcccgca gctggaaccg cctttatact cgcctgatgg
     2281 cagaaaacgg taactggctg gtagaagtga agccgtggta tgtggtgggt aatactgacg
     2341 ataacccgga tatcaccaaa tatatgggtt actaccagct taaaatcggc tatcacctcg
     2401 gtgatgcggt gctcagtgcg aaaggacagt acaactggaa caccggctac ggcggcgcgg
     2461 agttaggctt aagttacccg atcaccaaac atgtgcgcct ttatactcag gtttacagcg
     2521 gctatggcga atcgctcatc gactataact tcaaccagac ccgtgtcggt gtgggggtta
     2581 tgctaaacga tttgttttga tgaacggttg agtggttggc aaatctggaa tccagcatcc
     2641 aggattaccc tctcagagac taaaagcatt gcagtttctc gcgcaggcgc tgaaaatagc
     2701 gcctgttttt atttcaggca atcggggtga atgtggcgca ggcggaagtg ttgaatctgg
     2761 agtccggagc taaacaggtt ttacaagaaa cctttggcta ccaacagttt cgcccgggcc
     2821 aggaagaaat tatcgacact gtgctttccg gtcgcgattg cctggtcgtc atgcccaccg
     2881 gtggcggaaa atccctttgc tatcaaattc ctgccttatt gctaaacggc cttaccgtgg
     2941 ttgtttcacc gctgatttcg ttgatgaaag atcaggtgga tcaactgcaa gccaacggcg
     3001 tggcggcggc gtgccttaac tcgacgcaaa cccgcgaaca gcaacttgaa gtgatgacag
     3061 gctgccgcac cgggcaaatt cgtctgcttt atatcgcccc ggaacgcctg atgctggata
     3121 actttcttga gcatctggcg cactggaatc cggtgttatt agccgttgat gaagcgcact
     3181 gtatctccca atggggccac gatttccgcc cggaatatgc cgcgctcggt cagttgcgcc
     3241 agcggttccc gacgctgccg tttatggcgc tgaccgccac agccgacgac accacgcgcc
     3301 aggatatcgt gcgcctgctg gggctgaacg atccgctgat tcaaatcagc agttttgacc
     3361 gtccgaatat tcgctacatg ctgatggaga aattcaaacc gctcgatcag ttgatgcgct
     3421 acgtgcagga acagcgcggt aagtccggca ttatctactg caacagccgg gcgaaagtag
     3481 aagacaccgc tgcgcgcctg caaagcaagg gtattagcgc ggcggcctat catgccgggc
     3541 tggaaaataa tgtccgcgcc gacgtgcagg agaaattcca gcgcgatgac ctgcaaattg
     3601 tggtggcgac ggtggcgttc ggcatgggca tcaataaacc aaacgttcgc ttcgtggtcc
     3661 actttgatat tccgcgcaat atcgaatcct attatcagga aaccggtcgc gccgggcgtg
     3721 atggcctgcc tgcggaagcg atgctgtttt acgatccggc tgatatggcg tggctgcgcc
     3781 gttgtctgga agagaagccg caggggcagt tgcaggatat cgagcgccac aaactcaatg
     3841 cgatgggcgc gtttgccgaa gcgcaaactt gccgtcgtct ggtattgctg aactattttg
     3901 gcgaagggcg tcaggagccg tgcgggaact gcgatatctg cctcgatccg ccgaaacagt
     3961 acgacggttc aaccgatgct cagattgccc tttccaccat tggtcgtgtg aatcagcggt
     4021 ttgggatggg ttatgtggtg gaagtgattc gtggtgctaa taaccagcgt atccgcgact
     4081 atggtcatga caaactgaaa gtctatggca tgggccgtga taaaagccat gaacattggg
     4141 tgagcgtgat ccgccagctg attcacctcg gcctggtgac gcaaaatatt gcccagcatt
     4201 ctgccctaca actgacagag gccgcgcgcc cggtgctgcg cggcgaatcc tctttgcaac
     4261 ttgccgtgcc gcgtatcgtg gcgctcaaac cgaaagcgat gcagaaatcg ttcggcggca
     4321 actatgatcg caaactgttc gccaaattac gcaaactgcg taaatcgatt gccgatgaaa
     4381 gtaatgtccc gccgtacgtg gtgtttaacg acgcaacctt gattgagatg gctgaacaga
     4441 tgccgatcac cgccagcgaa atgctcagcg ttaacggcgt tgggatgcgc aagctggaac
     4501 gctttggtaa accgtttatg gcgctgattc gtgcgcatgt cgatggcgac gacgaagagt
     4561 agtcagcagc ataaaaaagt gccaggatga agactccgta aacgtttccc ccgcgagtca
     4621 aatgtatgtt gatgttattt ctcaccgtcg ccatggtgca cattgtggcg cttatgagtc
     4681 ccggtcccga tttctttttt gtctctcaga ccgctgtcag tcgttcccgt aaagaagcga
     4741 tgatgggcgt gctgggtatt acctgcggcg ttatggtttg ggcagggatt gcgttgcttg
     4801 gcctgcattt gattatcgaa aaaatggcct ggctgcatac gctgattatg gttggcggtg
     4861 gcctgtatct ctgctggatg ggttaccaga tgctgcgcgg cgcgctgaaa aaagaggcgg
     4921 tttctgcacc tgcgccacag gtcgagctgg cgaaaagtgg gcgcagtttc ctgaaaggtt
     4981 tactgaccaa tctcgctaat ccgaaagcga ttatctactt tggctcggtg ttctcattgt
     5041 ttgtcggtga taacgttggc actaccgcgc gctggggcat ttttgcgctg atcattgtcg
     5101 aaacactggc gtggtttacc gtcgttgcca gcctgtttgc cctgccgcaa atgcgccgtg
     5161 gttatcaacg tctggcgaag tggattgatg gttttgccgg ggcgttattt gccggatttg
     5221 gcattcattt gattatttcg cggtgatgcc agacgcgtct tcagagtaag tcggataagg
     5281 cgtttacgcc gcatccgaca ttatttttca cgcatgcctc gccgatgcta acagcgctcc
     5341 caccagcata aacaacgagc cgaaaatctt attcagcgcc ttcatctgct ttggtccttt
     5401 aatccataga gcaatccgtt gagcaagggt ggcgtaaccg atcatcacaa taatatcgac
     5461 cacaatagtg gtgacgccga gcacgatata ctgcatcagt tgcggctgtt gcggcatgat
     5521 gaattgcgga aatagcgcgg ccagaaacac aatacttttg ggattggtga gattcacaaa
     5581 aactgcgcgc tggaacaaat gtcgacgcga ttgagtagag gccagcgatt taagatcaat
     5641 tgcgccagcg gcgcgccatt gctgtattcc cagccaaatc aagtaagccg cgcctgccca
     5701 cttcaacact tcaaacgcaa tcactgagcg ggaaaatagc gtccccaacc ccacgccaac
     5761 cagcacaata tgaatcgcca gtccggtctg aagcccagca atagacgcca ccgcgccgcg
     5821 ataaccgtgg ttgagcgagg tggtcatagt gttgattgca ccagagcctg gcgacaggct
     5881 taaaatgatc gatgtcagca ggtaggcaaa ccaccattct aaggtcatga tgaactcccg
     5941 gtgtgtctat ttttgtgcca caatacgcta ctgtcgcagc gttgtgtcag gcacgctaaa
     6001 aaaaacgatt ttacgtggtt taagaggcag attacccgat gtttcagcag caaaaagact
     6061 gggaaacaag agaaaacgcg tttgctgctt ttaccatggg accgctgact gatttctggc
     6121 gtcagcgtga tgaagcagag tttactggtg tggatgacat tccggtgcgc tttgtccgtt
     6181 ttcgcgcaca gcaccatgac cgggtggtag tcatctgccc ggggcgtatt gagagctacg
     6241 taaaatatgc ggaactggcc tacgatttgt tccatttggg gtttgatgtc ttaatcatcg
     6301 accatcgcgg gcagggacgt tccggtcgcc tgttagccga tccgcatctc gggcatgtta
     6361 atcgctttaa tgattatgtt gatgatctgg cggcattctg gcaacaggag gtccagcccg
     6421 gtccgtggcg taaacgctat atactggcac attcgatggg cggtgcgatc tccacattat
     6481 ttctgcaacg ccatccaggt gtatgtgacg ccattgcgct aactgcgcca atgtttggga
     6541 tcgtgattcg tatgccgtca tttatggcac ggcagatcct caactgggcc gaagcgcatc
     6601 cacgtttccg tgatggctat gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta
     6661 tcaacgtact gacccacagc agacagcgat atcgacgtaa cttacgcttc tatgctgatg
     6721 acccaacgat tcgcgtcggt gggccgacct accattgggt acgcgaaagt attctggctg
     6781 gcgaacaggt gttagccggt gcgggtgatg acgccacgcc aacgcttctc ttgcaggctg
     6841 aagaggaacg cgtggtggat aaccgcatgc atgaccgttt ttgtgaactc cgcaccgccg
     6901 cgggccatcc tgtcgaaggg ggacgaccgc tggtaattaa aggtgcgtac catgagatcc
     6961 tttttgaaaa ggacgcaatg cgctcagttg cgctccacgc catcgttgat tttttcaaca
     7021 ggcataactc acccagcgga aaccgctcta cagaggttta aatttcttat gtaccaggtt
     7081 gttgcgtctg atttagatgg cacgttactt tctcccgacc atacgttatc cccttacgcc
     7141 aaagaaaccc tgaagctgct caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt
     7201 caccacgttg atgtggggca aattcgcgat aatctggaga ttaagtctta catgattacc
     7261 tccaatggtg cgcgcgttca cgatctggat ggtaatctga tttttgctca taacctggat
     7321 cgcgacattg ccagcgatct gtttggcgta gtcaacgaca atccggacat cattactaac
     7381 gtttatcgcg acgacgaatg gtttatgaat cgccatcgcc cggaagagat gcgctttttt
     7441 aaagaagcgg tgttcaaata tgcgctgtat gagcctggat tactggagcc ggaaggtgtc
     7501 agcaaagtgt tcttcacctg cgattcccat gaacaactgc tgccgctgga gcaggcgatt
     7561 aacgctcgtt ggggcgatcg cgtcaacgtc agtttctcta ccttaacctg tctggaagtg
     7621 atggcgggcg gcgtttcaaa aggccatgcg ctggaagcgg tggcgaagaa actgggctac
     7681 agcctgaagg attgtattgc gtttggtgac gggatgaacg atgccgaaat gctgtcgatg
     7741 gcggggaaag gctgcattat gggcagtgcg caccagcgtc tgaaagacct tcatcccgag
     7801 ctggaagtga ttggtactaa tgccgacgac gcggtgccgc attatctgcg taaactctat
     7861 ttatcgtaat cgttctttat ttggtcagtt gtcaacctga tacttcgcta caatggatac
     7921 tcgttaatca aagagttttc cattgtggcg ctacttatca tcaccacgat tttgtgggcc
     7981 ttctccttta gtttttatgg cgagtacctt gcggggcacg tcgatagcta ttttgcggtg
     8041 ctggtgcgcg ttggcctggc ggcactcgtt tttctgccgt ttctgcgtac ccgtggcaat
     8101 agcctgaaaa cagtcggcct gtatatgctg gtgggcgcga tgcagctcgg cgtgatgtat
     8161 atgctgagtt tccgcgctta tctctacctg acggtttccg aactgctgct gttcaccgta
     8221 ctgacgccgc tctacatcac gctgatttat gacatcatga gtaagcgccg tctgcgctgg
     8281 ggatatgctt ttagtgcctt gttggcggtg attggtgccg ggattattcg ctatgatcag
     8341 gtcaccgacc atttctggac tggcttgttg ctggtgcaac tctccaatat cacttttgcc
     8401 atcggcatgg tgggttacaa acgcctgatg gaaactcgcc cgatgccaca gcataacgcc
     8461 tttgcgtggt tctatcttgg cgcgttcctg gtggcggtaa ttgcatggtt cttgctggga
     8521 aatgcgcaga aaatgccgca aaccacgttg caatggggca ttctggtgtt tcttggcgtg
     8581 gtggcttccg ggattggcta ctttatgtgg aactacggcg cgacgcaggt ggacgccgga
     8641 acgctgggaa ttatgaataa tatgcacgtt ccggcagggc tgctggtaaa cctggctatc
     8701 tggcaccaac agccgcactg gccaacgttt attacaggcg cgctggtgat cctggcctca
     8761 ctgtgggtgc atcgtaagtg ggtcgctccg cgctcttcac aaacggcaga tgatcgcagg
     8821 cgtgattgcg cgctgagcga ataaacgctt ccgtaactgg ctgacgctgc tcgccatcgc
     8881 gcacggcggc gtacagtcgg ctccacaagc cttcgcccag ggttttggtc accaccagac
     8941 cctggcgctc aaaactctct actacccaat gcggcagggc ggcaataccc atccgcgcgg
     9001 caaccatctg aatcaacaat aaggtgttat cgacgctttt cagtgacggg cttacgcctg
     9061 ccggctgaag aaaatgccgc cagacatcca gtcggctacg ctgcaccgga taaatcaaca
     9121 gcgtctcgct ggcgagatct tccggtgtaa tacgcgtttt tgccgccaat ggatggtcag
     9181 gtgctaacac caggcgtact tcataatcga acatcggcga ataatgcagg ccactgcgcg
     9241 gcaggatatc ggaggtcatc accagatcca gctcgccctg ttgcaacgcg ggctgcgggt
     9301 caaacgtcac gcccgattta aaatccatct ctacctgcgg ccagttctta tggaaatttt
     9361 ctaacgcagg cgtcagccac tgaatacagc tatggcactc aatggcaatg cgcagacgcg
     9421 tctgctgcgg ttcattgcag gcttgcagtg cctggctgat ttgcggcagc acctggtttg
     9481 ccagttgcaa caggatttct ccctgcggtg taaagcgtag cggctggctc ttacgcacaa
     9541 agagccggaa gccaaggcgt tgttccagat cgctaaactg gtgagacagg gcggattgcg
     9601 tctgatgcaa cgtcgccgca gcggctgcga gagagccgca gttccgcaac gcttgtagcg
     9661 ttttcaggtg ttttacttcg atcatgaaag tccttcactt cggcatgaat aatttgcgct
     9721 tgaggaatat acagtaaccg ccaattatgg atgtgtaaac atctggacgg ctaaaatcct
     9781 tcgtctttta aatttatggt gcgttggctg cgtttctcca ccccggtcac ttacttcagt
     9841 aagctcccgg ggatgaataa acttgccgcc ttccctaaat tcaaaatcca taggatttac
     9901 atataattag aggaagaaaa atgacaatat tgaatcacac cctcggtttc cctcgcgttg
     9961 gcctgcgtcg cgagctgaaa aaagcgcaag agagttattg ggcggggaac tccacgcgtg
    10021 aagaactgct ggcggtaggg cgtgaattgc gtgctcgtca ctgggatcaa caaaagcaag
    10081 cgggtatcga cctgctgccg gtgggcgatt ttgcctggta cgatcatgta ctgaccacca
    10141 gtctgctgct gggtaacgtt ccggcgcgtc atcagaacaa agatggttcg gtagatatcg
    10201 acaccctgtt ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg
    10261 aaatgaccaa atggtttaac accaactatc actacatggt gccggagttc gttaaaggcc
    10321 aacagttcaa actgacctgg acgcagctgc tggacgaagt ggacgaggcg ctggcgctgg
    10381 gccacaaggt gaaacctgtg ctgctggggc cgattacctg gctgtggctg gggaaagtga
    10441 aaggtgaaca gtttgaccgc cttagcctgc tgaacgacat tctgccggtt tatcagcaag
    10501 tgctggcaga actggcgaaa cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg
    10561 tactggaact accacaggcg tggctggacg catacaaacc cgcttacgac gcgctccagg
    10621 gacaggtgaa actgctgctg accacctatt ttgaaggcgt aacgccaaat ctcgacacga
    10681 ttactgcgct gcctgttcag gggctgcatg tggaccttgt acatggtaaa gatgacgttg
    10741 ttgaactgca caagcgcctg ccttctgact ggctgctgtc tgcgggtctg atcaatggtc
    10801 gtaacgtctg gcgcgccgat cttactgaga aatatgcgca aattaaggac attgtcggca
    10861 aacgtgattt gtgggtggca tcatcctgct cgttgctgca cagccccatc gacctgagcg
    10921 tggaaacgcg tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc
    10981 atgaactggc actgctgcgc gatgcgctga acagtggtga cacggcagct ctggcagagt
    11041 ggagcgcccc aattcaggcg cgtcgtcact ctactcgtgt acataacccg gcagtagaaa
    11101 agcgtctggc ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg
    11161 ccgaagccca gcgtgcgcgt tttaaactgc cagcgtggcc gaccaccacg attggttcct
    11221 tcccgcaaac cacggaaatt cgtaccctgc gtctggattt caaaaagggc aatctcgacg
    11281 ccaataacta ccgcacgggc attgcggaac atatcaggca ggccattgtt gaacaggaac
    11341 gtttgggact ggatgtgctg gtacatggcg aggccgagcg taatgacatg gtggaatatt
    11401 ttggcgagca cctcgacggg tttgtcttta cgcaaaacgg ttgggtacag agctatggtt
    11461 cccgctgcgt gaagccaccg attgtcattg gtgatattag ccgcccggca ccgattaccg
    11521 tggagtgggc gaagtatgcg caatctctga ctgataaacc ggtgaaaggg atgctgactg
    11581 gcccggtgac cattctctgc tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg
    11641 ccaaacaaat tgcgctggcg ctgcgtgatg aagtggcgga cctggaagcc gcaggaattg
    11701 gcatcatcca gattgatgaa ccggcgctgc gcgaaggttt accgctgcgt cgtagcgact
    11761 gggatgcata tctccagtgg ggcgtggagg ccttccgtat caacgccgcc gtggcgaaag
    11821 atgacacaca aatccacact cacatgtgtt attgcgagtt caacgacatc atggattcga
    11881 ttgcggcact ggatgcagac gtcatcacca tcgaaacctc gcgttccgac atggagttgc
    11941 tggagtcgtt tgaagagttt gattatccga atgaaatcgg tccaggcgtc tatgacattc
    12001 actcgccaaa cgtaccgagc gtagaatgga ttgaagcctt gctgaagaaa gcagcacaac
    12061 gcattccggc agagcgcctg tgggttaacc cggactgtgg tctgaaaacg cgcggctggc
    12121 cagaaacccg cgccgcgctg gcgaacatgg tacaggcggc gcagaatttg cgtcgggggt
    12181 aaaatccaaa ccgggtggta ataccacccg gtcttttctc attacagcga cttcttccca
    12241 ccatactgct taaaccattc cagcatacgc tgccagccat ctttcgcaga ttcggcatga
    12301 tagctcgggc gataatcagc gttgaatgca tgcccggcat ccgggtacac gataatctct
    12361 gctttcgcat tagcagcccg cagcgcctgg cgcatggttt caacgctctc ctgcggaatg
    12421 ctgttatcct gactgccata taagccgaga accggcgcgt taagatcggt tgcgatatca
    12481 acaggttgtt tcggtgaatt cagcgacttg tcgcccgtca gtttgccgta ccacgccact
    12541 gcggctttta gctgtggatt atgcgcggca tacagccagg tgatacgtcc accccagcag
    12601 aatccggtga tcattaaacg atgaacatcg ccgccgttgc gggaagccca actggcgaca
    12661 tgatcgagat cggccagcac ctgcgagtca ggcacttttg ctaccagacc gctaagcaac
    12721 gtggggatat cggcaaaatc attcggatcg ccttcgcgga agtaaagttc aggtgcgata
    12781 gccagatacc cctccagcgc cagacggcga caaatgtcgc ggatatgttc atgcacgcca
    12841 aaaatttcct gcactacaat gaccactggc agtgggccat cgctttgctt tggtctggca
    12901 tggtaagcag gcatgttatc cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg
    12961 tcgtccgggg tctgaacgat ggtcgaagcg agaggcgatg cagcaggtgc aaatccagat
    13021 tgttgtgttg ttgccatggt attctccgta cccttataaa aatgttgcgc aatgttaact
    13081 atagtcagca tgcaacaaat cacattgcct gaatcggctc atcttttatg cagtcctgca
    13141 gaatgaagag tgatttatgt gatttgaatc acttttggtg ggtaaattta tgcaacgcat
    13201 ttgcgtcatg gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt
    13261 gcttcttctg actaaaccga ttcacagagg agttgtatat gtccaagtct gatgtttttc
    13321 atctcggcct cactaaaaac gatttacaag gggctacgct tgccatcgtc cctggcgacc
    13381 cggatcgtgt ggaaaagatc gccgcgctga tggataagcc ggttaagctg gcatctcacc
    13441 gcgaattcac tacctggcgt gcagagctgg atggtaaacc tgttatcgtc tgctctaccg
    13501 gtatcggcgg cccgtctacc tctattgctg ttgaagagct ggcacagctg ggcattcgca
    13561 ccttcctgcg tatcggtaca acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc
    13621 tggttaccac ggcgtctgtc cgtctggatg gcgcgagcct gcacttcgca ccgctggaat
    13681 tcccggctgt cgctgatttc gaatgtacga ctgcgctggt tgaagctgcg aaatccattg
    13741 gagcgacaac tcacgttggc gtgacagctt cttctgatac cttctaccca ggtcaggaac
    13801 gttacgatac ttactctggt cgcgtagttc gtcactttaa aggttctatg gaagagtggc
    13861 aggcgatggg cgtaatgaac tatgaaatgg aatctgcaac cctgctgacc atgtgtgcaa
    13921 gtcagggcct gcgtgccggt atggtagcgg gtgttatcgt taaccgcacc cagcaagaga
    13981 tcccgaatgc tgagacgatg aaacaaaccg aaagccatgc ggtgaaaatc gtggtggaag
    14041 cggcgcgtcg tctgctgtaa ttctcttctc ctgtctgaag gccgacgcgt tcggcctttt
    14101 gtatttttgc gtagcgcctc gcaggaaatg cctttccaac tggacgtttg tacagcacaa
    14161 ttctattttg tgcgggtaag ttgttgcgtc aggaggcgtt gtggatttct caatcatggt
    14221 ttacgcagtt attgcgttgg tgggtgtggc aattggctgg ctgtttgcca gttatcaaca
    14281 tgcgcagcaa aaagccgagc aattagctga acgtgaagag atggtcgcgg agttaagcgc
    14341 ggcaaaacaa caaattaccc aaagcgagca ctggcgtgca gagtgcgagt tactcaataa
    14401 cgaagtgcgc agcctgcaaa gtattaacac ctctctggag gccgatctgc gtgaagtaac
    14461 cacgcggatg gaagccgcac agcaacatgc tgacgataaa attcgccaga tgattaacag
    14521 cgagcagcgc ctcagtgagc agtttgaaaa cctcgccaac cgtatttttg agcacagcaa
    14581 tcgccgggtt gatgagcaaa accgtcagag tctgaacagc ctgttgtcgc cgctacgtga
    14641 acaactggac ggtttccgcc gtcaggttca ggacagcttc ggtaaagaag cacaagaacg
    14701 ccataccctg acccacgaaa ttcgcaatct ccagcaactc aacgcgcaaa tggcccagga
    14761 agcgatcaac ctgacgcgcg cgctgaaagg cgacaataaa acccagggca actggggcga
    14821 ggtagtattg acgcgggtgc tggaggcttc cggtctgcgt gaagggtatg aatatgaaac
    14881 ccaggtcagc atcgaaaatg acgcccgctc gcggatgcag ccggatgtca tcgtgcgcct
    14941 gccgcaggga aaagatgtgg tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta
    15001 ttttaacgcc gaagacgact acacccgcga aagcgcgcta caggaacata tcgcgtcggt
    15061 gcgtaaccat atccgtttgc tgggacgcaa agattatcaa cagctgccgg ggctgcgaac
    15121 tctggattac gtgctgatgt ttattcccgt tgaacccgct tttttactgg cgcttgaccg
    15181 ccagccggag ctgatcaccg aagcgttgaa aaacaatatc atgctggtta gcccgactac
    15241 gctgctggtg gcgctgcgca ctatcgccaa cctgtggcgt tatgagcatc aaagccgcaa
    15301 cgcccagcaa atcgccgatc gtgccagcaa gctgtacgac aagatgcgtt tgttcatcga
    15361 tgacatgtcc gcgattggtc aaagtctcga caaagcgcag gataattatc ggcaggcaat
    15421 gaaaaaactc tcttcagggc gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt
    15481 aggagtagaa attaaacgcg agattaattc ggatttggct gaacaggcgg tgagccagga
    15541 tgaagagtat cgacttcggt cggttccgga gcagccgaat gatgaagctt atcaacgcga
    15601 tgatgaatat aatcagcagt cgcgctagcc cattgggagt agttaagccg ggtagaaatc
    15661 tagggcatcg acgcccaatc tgttacactt ctggaacaat tttttgatga gcaggcattg
    15721 agatggtgga taagtcacaa gaaacgacgc actttggttt tcagaccgtc gcgaaggaac
    15781 aaaaagcgga tatggtcgcc cacgttttcc attccgtggc atcaaaatac gatgtcatga
    15841 atgatttgat gtcatttggt attcatcgtt tgtggaagcg attcacgatt gattgcagcg
    15901 gcgtacgccg tgggcagacc gtgctggatc tggctggtgg caccggcgac ctgacagcga
    15961 aattctcccg cctggtcgga gaaactggca aagtggtcct tgctgatatc aatgaatcca
    16021 tgctcaaaat gggccgcgag aagctgcgta atatcggtgt gattggcaac gttgagtatg
    16081 ttcaggcgaa cgctgaggcg ctgccgttcc cggataacac ctttgattgc atcaccattt
    16141 cgtttggtct gcgtaacgtc accgacaaag ataaagcact gcgttcaatg tatcgcgtgc
    16201 tgaaacccgg cggccgcctg ctggtgcttg agttctcgaa gccaattatc gagccgctga
    16261 gcaaagccta tgatgcatac tccttccatg tgctgccgcg tattggctca ctggtcgcga
    16321 acgacgccga cagctaccgt tatctggcag aatccatccg tatgcatccc gatcaggata
    16381 ccctgaaagc catgatgcag gatgccggat tcgaaagtgt cgactactac aatctgacgg
    16441 caggggttgt ggcgctgcat cgtggttata agttctgaca ggagaccgga aatgcctttt
    16501 aaacctttag tgacggcagg aattgaaagt ctgctcaaca ccttcctgta tcgctcaccc
    16561 gcgctgaaaa cggcccgctc gcgtctgttg ggcaaagtgc tgcgcgtgga ggtaaaaggc
    16621 ttttcgacgt cattgattct ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg
    16681 gcaggcgatg ctgactgcac cgttatcgcc tacgccagtg tgttgccgaa acttcgcgat
    16741 cgccagcagc ttgccgccct gattcgcagt ggtgagctgg aagtgcaggg cgatattcag
    16801 gtggtgcaaa acttcgttgc gctggcagat ctggcagagt tcgaccctgc ggaactgctg
    16861 gccccttata ccggtgatat cgccgctgaa ggaatcagca aagccatgcg cggaggcgca
    16921 aagttcctgc atcacggcat taagcgccag caacgttatg tggcggaagc cattactgaa
    16981 gagtggcgta tggcacccgg tccgcttgaa gtggcctggt ttgcggagga gacggctgcc
    17041 gtcgagcgtg ctgttgatgc cctgaccaaa cggctggaaa aactggaggc taaatgacgc
    17101 caggtgaagt acggcgccta tatttcatca ttcgcacttt tttaagctac ggacttgatg
    17161 aactgatccc caaaatgcgt atcaccctgc cgctacggct atggcgatac tcattattct
    17221 ggatgccaaa tcggcataaa gacaaacctt taggtgagcg actacgactg gccctgcaag
    17281 aactggggcc ggtatggatc aagttcgggc aaatgttatc aacccgccgc gatctttttc
    17341 cgccgcatat tgccgatcag ctggcgttat tgcaggacaa agttgctccg tttgatggca
    17401 agctggcgaa gcagcagatt gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg
    17461 acgattttga aatcaagccg ctggcttctg cttctatcgc ccaggttcat accgcgcgat
    17521 tgaaatcgaa tggtaaagag gtggtgatta aagtcatccg cccggatatt ttgccggtta
    17581 ttaaagcgga tctgaaactt atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg
    17641 atggtcgccg tctgcgccca accgaagtgg tgcgcgagta cgaaaagaca ttgattgatg
    17701 aactgaattt gctgcgggaa tctgccaacg ccattcagct tcggcgcaat tttgaagaca
    17761 gcccgatgct ctacatcccg gaagtttacc ctgactattg tagtgaaggg atgatggtga
    17821 tggagcgcat ttacggcatt ccggtgtctg atgttgcggc gctggagaaa aacggcacca
    17881 acatgaaatt gctggcggaa cgcggcgtgc aggtgttctt cactcaggtc tttcgcgaca
    17941 gctttttcca tgccgatatg caccctggca acatcttcgt aagctatgaa cacccggaaa
    18001 acccgaaata tatcggcatt gattgcggga ttgttggctc gctaaacaaa gaagataaac
    18061 gctatctggc agaaaacttt atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc
    18121 tacacgtcga ttctggctgg gtgccaccag ataccaacgt tgaagagttc gaatttgcta
    18181 ttcgtacggt ctgtgaacct atctttgaga aaccgctggc cgaaatttcg tttggacatg
    18241 tactgttaaa tctgtttaat acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg
    18301 tgttactcca gaaaaccctg ctctacgtcg aaggggtagg acgccagctt tatccgcaac
    18361 tcgatttatg gaaaacggcg aagcctttcc tggagtcgtg gattaaagat caggtcggta
    18421 ttcctgcgct ggtgagagca tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag
    18481 aactgcctga actggtttac gacagtttgc gccagggcaa gtatttacag cacagtgttg
    18541 ataagattgc ccgcgagctt cagtcaaatc atgtacgtca gggacaatcg cgttattttc
    18601 tcggaattgg cgctacgtta gtattaagtg gcacattctt gttggtcagc cgacctgaat
    18661 gggggctgat gcccggctgg ttaatggcag gtggtctgat cgcctggttt gtcggttggc
    18721 gcaaaacacg ctgatttttt catcgctcaa ggcgggccgt gtaacgtata atgcggcttt
    18781 gtttaatcat catctaccac agaggaacat gtatgggtgg tatcagtatt tggcagttat
    18841 tgattattgc cgtcatcgtt gtactgcttt ttggcaccaa aaagctcggc tccatcggtt
    18901 ccgatcttgg tgcgtcgatc aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc
    18961 aggataaaac cagtcaggat gctgatttta ctgcgaaaac tatcgccgat aagcaggcgg
    19021 atacgaatca ggaacaggct aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt
    19081 aatccgtgtt tgatatcggt tttagcgaac tgctattggt gttcatcatc ggcctcgtcg
    19141 ttctggggcc gcaacgactg cctgtggcgg taaaaacggt agcgggctgg atccgcgcgt
    19201 tgcgttcact ggcgacaacg gtgcagaacg aactgaccca ggagttaaaa ctccaggagt
    19261 ttcaggacag tctgaaaaag gttgaaaagg cgagcctcac taacctgacg cccgaactga
    19321 aagcgtcgat ggatgaacta cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa
    19381 acgatcctga aaaggcgagc gatgaagcgc acaccatcca taacccggtg gtgaaagata
    19441 atgaagctgc gcatgagggc gtaacgcctg ccgctgcaca aacgcaggcc agttcgccgg
    19501 aacagaagcc agaaaccacg ccagagccgg tggtaaaacc tgctgcggac gctgaaccga
    19561 aaaccgctgc accttcccct tcgtcgagtg ataaaccgta aacatgtctg tagaagatac
    19621 tcaaccgctt atcacgcatc tgattgagct gcgtaagcgt ctgctgaact gcattatcgc
    19681 ggtgatcgtg atattcctgt gtctggtcta tttcgccaat gacatctatc acctggtatc
    19741 cgcgccattg atcaagcagt tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc
    19801 gccgttcttt acgccgatca agctgacctt tatggtgtcg ctgattctgt cagcgccggt
    19861 gattctctat caggtgtggg catttatcgc cccagcgctg tataagcatg aacgtcgcct
    19921 ggtggtgccg ctgctggttt ccagctctct gctgttttat atcggcatgg cgttcgccta
    19981 ctttgtggtc tttccgctgg catttggctt ccttgccaat accgcgccgg aaggggtgca
    20041 ggtatccacc gacatcgcca gctatttaag cttcgttatg gcgctgttta tggcgtttgg
    20101 tgtctccttt gaagtgccgg tggcaattgt gctgctgtgc tggatgggga ttacctcgcc
    20161 agaagactta cgcaaaaaac gcccgtatgt gctggttggt gcattcgttg tcgggatgtt
    20221 gctgacgccg ccggatgtct tctcgcaaac gctgttggcg atcccgatgt actgtctgtt
    20281 tgaaatcggt gtcttcttct cacgctttta cgttggtaaa gggcgaaatc gggaagagga
    20341 aaacgacgct gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc
    20401 ggttgtcata tggagtacag gatgtttgat atcggcgtta atttgaccag ttcgcaattt
    20461 gcgaaagacc gtgatgatgt tgtagcgcgc gcttttgacg cgggagttaa tgggctactc
    20521 atcaccggca ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg tcagtattca
    20581 tcctgttggt caacggcggg cgtacatcct cacgacagca gccagtggca agctgcgact
    20641 gaagaagcga ttattgagct ggccgcgcag ccagaagtgg tggcgattgg tgaatgtggt
    20701 ctcgacttta accgcaactt ttcgacgccg gaagagcagg aacgcgcttt tgttgcccag
    20761 ctacgcattg ccgcagattt aaacatgccg gtatttatgc actgtcgcga tgcccacgag
    20821 cggtttatga cattgctgga gccgtggctg gataaactgc ctggtgcggt tcttcattgc
    20881 tttaccggca cacgcgaaga gatgcaggcg tgcgtggcgc atggaattta tatcggcatt
    20941 accggttggg tttgcgatga acgacgcgga ctggagctgc gggaactttt gccgttgatt
    21001 ccggcggaaa aattactgat cgaaactgat gcgccgtatc tgctccctcg cgatctcacg
    21061 ccaaagccat catcccggcg caacgagcca gcccatctgc cccatatttt gcaacgtatt
    21121 gcgcactggc gtggagaaga tgccgcatgg ctggctgcca ccacggatgc taatgtcaaa
    21181 acactgtttg ggattgcgtt ttagagtttg cggaactcgg tgttcttcac actgtgctta
    21241 atctctttat taataagatt aagcaatagc atggagcgag cctcaccatc gggttcggtg
    21301 aaaatggcct gaaagccttc gaacgcgcct tcggtaataa tcaccttatc acccggataa
    21361 ggggttgccg gatcgacaat gtctttcggt ttatataccg atagctgatg aatcaccgcc
    21421 gatgggacta tcgctggcga cgcgccaaag cgcacgaagt ggctgacacc gcgggtcgcg
    21481 ttgatagtcg tggtatgaat cacttctgga tcaaattcca caaacaggta gttggggaac
    21541 aatggctcac tgactgcagt acgtttgcca cgcacgattt tttccagggt gatcatcggt
    21601 gccaggcaat tcacagcctg tctttcgagg tgttcctggg cacgttgaag ttgcccgcgc
    21661 ttgcagtaca gtaaatacca ggattgcata atgactctta tccgtttaat cggggcgcaa
    21721 ggatagcaaa agctttacgc taagttaatt atattccccg gtttgcgtta taccgtcaga
    21781 gttcacgcta atttaacaaa tttacagcat cgcaaagatg aacgccgtat aatgggcgta
    21841 gattaagagg ctacaatgga cgccatgaaa tataacgatt tacgcgactt cttgacgctg
    21901 cttgaacagc agggtgagct aaaacgtatc acgctcccgg tggatccgca tctggaaatc
    21961 actgaaattg ctgaccgcac tttgcgtgcc ggtgggcctg cgctgttgtt cgaaaaccct
    22021 aaaggctact caatgccggt gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg
    22081 ggcatggggc aggaagatgt ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg
    22141 aaagagccgg agccgccaaa aggtttccgc gacctgtttg ataaactgcc gcagtttaag
    22201 caagtattga acatgccgac aaagcggctg cgtggtgcgc cctgccaaca aaaaatcgtc
    22261 tctggcgatg acgtcgatct caatcgcatt cccattatga cctgctggcc ggaagatgcc
    22321 gcgccgctga ttacctgggg gctgacagtg acgcgcggcc cgcataaaga gcggcagaat
    22381 ctgggcattt atcgccagca gctgattggt aaaaacaaac tgattatgcg ctggctgtcg
    22441 catcgcggcg gcgcgctgga ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc
    22501 ccggtttctg tggcgctggg tgccgatccc gccacgattc tcggtgcagt cacccccgtt
    22561 ccggatacac tttcagagta tgcgtttgcc ggattgctgc gcggtaccaa gaccgaagtg
    22621 gtgaagtgta tctccaatga ccttgaagtg cccgccagtg cggagattgt gctggaaggg
    22681 tatatcgaac aaggtgaaac ggcgccggaa gggccgtatg gcgaccacac cggttactat
    22741 aacgaagtcg atagtttccc ggtatttacc gtgacgcata ttacccagcg tgaagatgcg
    22801 atttaccatt ccacctatac cgggcgtccg ccagatgagc ccgcggtact gggcgtggcg
    22861 ttgaacgaag tgtttgtacc gattctgcaa aaacagttcc cggaaattgt cgatttttac
    22921 ctgccgccgg aaggctgttc ttatcgtctg gcggtagtga cgatcaaaaa acagtacgcc
    22981 ggacacgcga agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt tatgtacact
    23041 aaatttgtga tcgtttgcga tgatgacgtc aacgcacgcg actggaacga tgtgatttgg
    23101 gcgattacca cccgtatgga cccggcgcgg gatactgttc tggtagaaaa tacgcctatt
    23161 gattatctgg attttgcctc gcctgtctcc gggctgggtt caaaaatggg gctggatgcc
    23221 acgaataaat ggccggggga aacccagcgt gaatggggac gtcccatcaa aaaagatcca
    23281 gatgttgtcg cgcatattga cgccatctgg gatgaactgg ctatttttaa caacggtaaa
    23341 agcgcctgat gcgcgtttgt tttgccctat ttatcgatcc gacagagaaa gcgcatgaca
    23401 accttaagct gtaaagtgac ctcggtagaa gctatcacgg ataccgtata tcgtgtccgc
    23461 atcgtgccag acgcggcctt ttcttttcgt gctggtcagt atttgatggt agtgatggat
    23521 gagcgcgaca aacgtccgtt ctcaatggct tcgacgccgg atgaaaaagg gtttatcgag
    23581 ctgcatattg gcgcttctga aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc
    23641 aaagatcatc aaatcgtggt cgatattccc cacggagaag cgtggctgcg cgatgatgaa
    23701 gagcgtccga tgattttgat tgcgggcggc accgggttct cttatgcccg ctcgattttg
    23761 ctgacagcgt tggcgcgtaa cccaaaccgt gatatcacca tttactgggg cgggcgtgaa
    23821 gagcagcatc tgtatgatct ctgcgagctt gaggcgcttt cgttgaagca tcctggtctg
    23881 caagtggtgc cggtggtaga acaaccggaa gcgggctggc gtgggcgtac tggcaccgtg
    23941 ttaacggcgg tattgcagga tcacggtacg ctggcagagc atgatatcta tattgccgga
    24001 cgttttgaga tggcgaaaat tgcccgtgac ctgttttgca gtgagcgtaa tgcgcgggaa
    24061 gatcgcctgt ttggcgatgc gtttgcattt atctgagata taaaaaaacc cgcccctgac
    24121 aggcgggaag aacggcaact aaaactttat tcagtggcat ttagatctat gtcgcatccg
    24181 gcattcggca cacgatgcct gatgcgacgc tggcgcgtct tatcaggcct acattggtgc
    24241 cggatcggta gaccggataa ggcgttcacg ccgcatccgg caagtggtta aacccgctca
    24301 aacaccgtcg caataccctg acccagaccg atacacatcg tcgccagacc aaactgaacg
    24361 tctttgtgtt ccatcagatt cagcagcgtg gtgctgatac gcgcaccgga acaacccagc
    24421 ggatgaccca gcgcgatcgc gccaccgttg aggttgatct tctcgtcaat ctgctcaatt
    24481 agtcccagat ctttaataca tggcaggatc tgcgcggcaa aggcttcatt catttcaaac
    24541 acgccgatat cactgacaga aagccccgct tttttcagcg ccagcttcga ggccggaacc
    24601 gggccgtaac ccataatcga tgggtcacaa ccaacgaccg ccatcgaacg cacacgagcg
    24661 cgcggcttaa gacctaattc atgggcgcgg ctttcactca tcaccagcat ggcagctgcg
    24721 ccatcggaaa gtgcagaaga tgtgcccgcc gttaccgtac cgtttactgg atcaaacgcc
    24781 ggacgcagcg tggcgagggc ttccacggtg gtttccgggc gaatcacttc gtcgtaatta
    24841 aactgcttca ggacgccgtc ggcatcgtga ccaccagttg ggatgatttc atttttaaat
    24901 gcggccgact gcgtggcggc ccaggcgcgg gcgtgtgacc gcgcggcaaa ggcatcctgc
    24961 atttcacggc tgataccgtg catacgcgcc agcatttctg ccgttaagcc catcatgccc
    25021 gccgctttgg cgacattgcg gctcaggccg gggtgaaaat cgacgccgtg actcatcggc
    25081 acatggccca tatgctccac gccgccaacc agacatgcct gcgcatcgcc ggtcatgatc
    25141 attcgtgctg cgtcatgcag tgcctgcatg gatgaaccac acaagcgatt gacagtaacc
    25201 gccgggacag agtgcggcac ttctgccagc agcgccgcgt tacgggcgat attaaaaccc
    25261 tgctccagcg tctgctgcac acaaccccag taaatatcgt cgagggccgc cgcttccagc
    25321 gccgggttac gcgccagcag gctacgcatt aaatgagcgg agagatcttc tgcacgcacg
    25381 ttacgaaaag caccgccctt cgaacggccc atcggggtgc gaattgcatc gacaatgaca
    25441 acctgttcca ttgtgactcc ttaagccgtt ttcaggtcgc caaccggacg ggctggctca
    25501 accgggggat agtacggttc gttatgacgc gctttattac gcagaccttc cggcacttcg
    25561 tacagcgggc cgaggtgctg atattgctgt gccatatcga ggtatttcgc gctgccgagg
    25621 gtgtccagcc agcggaacgc gccgccgtgg aacggaggga agcccaggcc gtagaccagc
    25681 gccatatccg cttctgccgg agtggcgata atgccttctt ccagacaacg caccacttcg
    25741 ttgaccatcg ggatcatcat gcgggcgata atctcttctt cgctgaaatc gcgcttcggc
    25801 tggctcactt ctactagcag gtcgtcaaca gcggcgtctt cttctttctt cggcttacct
    25861 ttgctgtctt ctttataacg ccagaaaccg aggccgttct tctgaccaaa gcggttggca
    25921 tcaaacagcg cgtcgatggc atcgcgataa tctttctgca tccgctgcgg gaagcctgct
    25981 gccatcaccg cctgagcgtg atgcgcggtg tcaatgccca cgacatccag cagatatgcc
    26041 ggacccatcg gccagccaaa ctgtttttcc atcactttgt cgatcttgcg gaaatccgcg
    26101 ccgtcgcgca gcagctggct gaaaccggcg aaatacggga acagcacgcg gttgacgaag
    26161 aagccggggc aatcgttaac cacaatcggc gttttaccca tcttgctcgc ccaggcgacg
    26221 actttcgcaa tagtttcgtc ggagcttttc tcgccgcgaa taatttccac caacggcatt
    26281 cggtggaccg ggttaaagaa gtgcatcccg cagaagtttt ccggacgttc cagcgcgttg
    26341 gccagttcgc tgataggaat ggttgaagtg ttagacgcca gcacggtatc cgggcgtact
    26401 ttctgctcgg tttctgccag tacggctttt ttcactttcg ggttttcgac aaccgcttct
    26461 accacaacat ccacgcggtc aaacccggcg tagtcgagcg ttgggtggat tgtagagatc
    26521 acgccagcca gtttcagacc atcgatcttg ccgcgctcaa gctgcttgtt cagcagtttc
    26581 gcggcttcgg tcatgccgag ggttaacgac ttgtcgttga tatctttcat gacaaccggc
    26641 acgcctttcc acgcagactg gtaagcgatg ccgccgccca taatgcctgc gcccagcaca
    26701 gcggcctgtt tcggggtttc aacgtctttg gtgagtttct tcgctttgcc ttttacatat
    26761 tgatcgttaa ggaatatgcc gaccagtgcg cgagcttcgt tggtatgcgc cagcgggaca
    26821 aaacttttgt tttccaggtt tagagcttct tcacgaccaa aacgggccgc agcttcaatg
    26881 gtttttactg cggtgatggg ggccggataa tgtttccccg ccgtttgtgc gaccatccct
    26941 ttagcgatgg tgaagctcat ggtagcttca atcttgctca gttttagcgg ttccagcttc
    27001 ggctgacgtt tcgctttcca gtcgaggtcg ccgttaatgg cctggcgtaa aatcgccatc
    27061 gccccttcaa tcagtttttc tgctttgact acgccatcca ccagaccaat tttcagcgcc
    27121 tggtccgcgc cgacatcttt accggcggca atgatttcca gcgcactgtc agcgcccagc
    27181 atacgtggca tacgtacaga accgccaaag cccggcatga tgcccagttt ggtttccggc
    27241 agaccgatgc gcagatccgg cgtcgccaga cgataatcgg tcgccagcac gcattcgcag
    27301 ccgccaccca gcgcgtagcc gttgacggca gcaatggtcg gcaccggcag atcttccagg
    27361 cgattaaaca cgctattggc aaaatgcagc cactggctta actgttcttc aggaacgagg
    27421 aacagggaca aaaattcggt gatatcagca ccgacgataa aggctgcttt gttcgaacgc
    27481 agcagcagcc cttttagatc tgattgctgt tccagcacgc cgatggcctc gccgaggctg
    27541 gcgacggtcg cagtgtcgag tttattaact gaacctgggg catcaaatac cagttcggca
    27601 atgccatctt ccagccagtc aaggtacagg gtgtcgcctt tgtaaagcat gtcagtctcc
    27661 tgaatccgca aggtgatctg gtcgtaccag atgagtcgaa gtgtgtattt tgtgttaaaa
    27721 atatgcaaac aaaagattaa agaaatgccg atctgatcac gctcggcaga aatcacgctc
    27781 tggatgaacg atgtgctaag atgcggagac ttaaggtcaa aaaaacagaa gggtaaaaaa
    27841 tggaatcact ggcctcgctc tataaaaatc atatagctac cttacaagaa cggactcgcg
    27901 atgcgctggc gcgcttcaag ctggatgcgt tacttattca ctccggcgag ctgttcaatg
    27961 tttttctcga cgatcatccc tatccgttta aagtgaaccc gcaattcaaa gcgtgggtgc
    28021 cggtaactca ggtgccaaac tgctggttgc tggtggatgg cgtgaacaag ccgaaactgt
    28081 ggttttatct gccggttgat tactggcaca acgtcgaacc gctgccgacc tccttctgga
    28141 ctgaagatgt ggaagtgatc gcgctgccga aagccgatgg cattggtagc ctgctgcctg
    28201 ctgcgcgcgg caatatcggt tatatcggtc cggtgccgga gcgtgcgctg caactgggta
    28261 ttgaggccag caacatcaac ccgaaagggg ttatcgacta cctgcattac tatcgctcct
    28321 tcaaaaccga gtacgaactg gcctgtatgc gtgaagcgca gaaaatggcg gtcaacggtc
    28381 atcgtgcggc agaagaagcg ttccgttctg gcatgagcga gttcgatatc aacattgcct
    28441 atctgaccgc gactggtcat cgtgataccg acgtacctta cagcaacatt gtggcgctta
    28501 acgaacacgc tgcggtgctg cattacacca aactggatca tcaggcgccg gaagagatgc
    28561 gcagcttcct gctggatgcc ggggccgaat ataacggcta tgccgctgac ctgacccgta
    28621 cctggtcggc aaaaagtgac aacgattacg cacagctggt ggaagacgta aatgatgaac
    28681 aactggcgct gatcgcgacc atgaaagctg gcgttagcta tgtggattac cacatccagt
    28741 tccatcagcg catcgccaaa ttgctgcgta aacatcaaat catcaccgat atgagtgaag
    28801 aagcgatggt cgaaaacgat ctcaccggac cgtttatgcc gcacggtatc ggccatccgc
    28861 tgggcctgca ggtgcatgac gtcgccggtt ttatgcagga tgatagcggt acgcacctcg
    28921 cggcaccggc aaaatatccg tacctgcgct gcacccgtat tctccagccg ggcatggtgt
    28981 taaccatcga accgggtatc tacttcattg aatcgctact ggcaccgtgg cgtgaagggc
    29041 agttcagcaa gcacttcaac tggcagaaaa ttgaagcact gaaaccgttc ggcggcattc
    29101 gtatcgaaga caacgtggtg atccacgaaa ataacgtgga aaacatgacc cgggatctga
    29161 aactggcgtg atggaaagct ggttaattcc tgcggcaccg gtcacggtcg ttgaagagat
    29221 caaaaaaagc cgcttcataa cgatgttggc gcataccgat ggcgttgagg cggcgaaagc
    29281 gtttgttgaa tcggtgcggg cagaacatcc cgatgcccgc caccattgtg tggcgtgggt
    29341 ggcgggtgcg ccggatgatt cacaacagtt gggtttctct gacgacgggg aaccggcggg
    29401 aacggcaggt aaaccgatgc tcgcccaact gatgggcagc ggcgtcgggg aaattaccgc
    29461 cgtggtggtg cgctactacg gcggcatatt gcttggcacc ggtgggttag tgaaagcgta
    29521 tggcggcggc gtgaatcagg cgctgcggca gctaacgacc caacgcaaga cgccattaac
    29581 cgaatatact ttgcaatgtg aatatagtca gttaaccggc attgaagcgt tgctggggca
    29641 gtgtgacggc aaaattatca acagtgatta tcaggcattc gttctgctgc gggtggcgct
    29701 tccggcggcg aaagtggctg aattttccgc aaagctggcg gattttagcc gtggttcatt
    29761 gcaattgtta gcgattgaag aataatcccc acttcgtttt gcagaactaa ggaagcggca
    29821 gagatgcatt ttcgcgccat tacccgaatc gttggactac tggtcatctt attttcaggg
    29881 accatgatta tccctgggct ggtagcactc atctaccggg atggagcggg ccgcgctttt
    29941 acccagacct tttttgtcgc cctcgccatt ggctctatgc tgtggtggcc gaaccgcaaa
    30001 gagaagggcg aactgaaatc ccgtgagggg tttctgatag tggtgctgtt ctggaccgtg
    30061 ctgggtagtg tcggtgcgct cccttttatc ttctcggaaa gcccgaacct cacgattacc
    30121 gatgcgtttt ttgaatcttt ctctggcctg accaccaccg gggccactac gctggtgggg
    30181 ctggattcgc tccctcacgc catcctcttt tatcgccaga tgctgcaatg gtttggcggg
    30241 atggggatca tcgtgttagc ggttgcgata ctgcctatcc tcggcgtggg tgggatgcag
    30301 ctctatcgtg cagaaatgcc cggcccgctg aaagataaca aaatgcgccc gcgaattgcg
    30361 gaaacggcga aaaccctgtg gttgatttat gtcttgctga ccgtcgcctg tgcgctggcg
    30421 ttgtggtttg ccggaatgga tgcctttgat gccatcggcc atagctttgc gactatcgct
    30481 attggcggct tctcgacaca tgatgccagt atcggttatt tcgatagccc gactattaac
    30541 actatcattg ctatcttcct gctgatctcc ggctgtaact acggtctgca cttttcactg
    30601 ttaagtgggc gtagtctgaa ggtttattgg cgcgatccgg aatttcgcat gtttatcggc
    30661 gtacagttta cgctggtggt tatttgtacc ctcgtactgt ggtttcataa tgtctacagt
    30721 tcggcgctga tgacaattaa ccaggcgttt ttccaggtgg tgtcgatggc gacaaccgcc
    30781 gggtttacga ctgacagcat tgcccgctgg ccgctctttt tgccggtact gcttttatgt
    30841 tcagctttta tcggcggttg tgccgggtca acgggcggtg gcctgaaagt gatccgcatc
    30901 ctgctgctgt ttaggcaggg gaaccgtgag ctgaaacgac tggtgcatcc gaacgccgtc
    30961 tatagcatta agctggggaa tcgcgcactg ccggaacgta tcctcgaagc cgtctgggga
    31021 tttttctccg cctatgcatt ggtgtttatt gtcagtatgc tggcgattat cgccacgggc
    31081 gtggatgact tttctgcctt tgcctcggtt gttgcgacat tgaataacct ggggccaggg
    31141 cttggcgtgg ttgctgataa ctttaccagc atgaacccgg tggctaaatg gatcctgatt
    31201 gccaacatgc tgtttggccg tctcgaggtc tttacattgc tggtgctctt taccccgact
    31261 ttctggcgtg aatgatggag taatacgtga aaacattaat tcttttctca acaagggacg
    31321 gacaaacgcg cgagattgcc tcctacctgg cttcggaact gaaagaactg gggatccagg
    31381 cggatgtcgc caatgtgcac cgcattgaag aaccacagtg ggaaaactac gaccgtgtgg
    31441 tcattggtgc ttctattcgc tatggtcact accactctgc gttccaggaa tttgtcaaaa
    31501 aacatgcgac gcgactgaat tcgatgccga gcgcctttta ttccgtaaac ctggtggcgc
    31561 gcaaaccgga gaagcgtact ccacagacca acagctacgc gcggaagttt ctgatgaact
    31621 cgcaatggcg tcccgatcgc tgcgcggtca ttgccggggc gctgcgttac ccacgttatc
    31681 gctggtacga ccgttttatg atcaagctga ttatgaagat gtcaggcggt gaaacggata
    31741 cgcgcaaaga agttgtctat accgattggg agcaggtggc gaatttcgcc cgagaaatcg
    31801 cccatttaac cgacaaaccg acgctgaaat aagcataaag aataaaaaat gcgcggtcag
    31861 aaaattattt taaatttcct cttgtcaggc cggaataact ccctataatg cgccaccact
    31921 gacacggaac aacggcaaac acgccgccgg gtcagcggga ttctcctgag aattccggca
    31981 gagaaagcaa aaataaatgc ttgactctgt agcgggaaag tgtattatgc acaccccgcg
    32041 ccgctgagaa aaagcgaagc ggcactgctc tttaacaatt tatcagacaa tctgtgtggg
    32101 cactcgaaga tacggattct taacgtcgca agacgaaaaa tgaat
//
LOCUS       DAZFKF010000052        31872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFKF010000052 DAZFKF010000000
VERSION     DAZFKF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.052"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..980)
                     /locus_tag="ACHKA1_002855"
     rRNA            complement(<1..980)
                     /locus_tag="ACHKA1_002855"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1597..3186
                     /gene="purH"
                     /locus_tag="ACHKA1_002856"
     CDS             1597..3186
                     /gene="purH"
                     /locus_tag="ACHKA1_002856"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO5680480.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            3198..4487
                     /gene="purD"
                     /locus_tag="ACHKA1_002857"
     CDS             3198..4487
                     /gene="purD"
                     /locus_tag="ACHKA1_002857"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO5680481.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(4484..5809)
                     /gene="zraR"
                     /locus_tag="ACHKA1_002858"
     CDS             complement(4484..5809)
                     /gene="zraR"
                     /locus_tag="ACHKA1_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO5680482.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(5806..7182)
                     /gene="zraS"
                     /locus_tag="ACHKA1_002859"
     CDS             complement(5806..7182)
                     /gene="zraS"
                     /locus_tag="ACHKA1_002859"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO5680483.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            7420..7845
                     /gene="zraP"
                     /locus_tag="ACHKA1_002860"
     CDS             7420..7845
                     /gene="zraP"
                     /locus_tag="ACHKA1_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO5680484.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            complement(7847..8542)
                     /gene="yjaH"
                     /locus_tag="ACHKA1_002861"
     CDS             complement(7847..8542)
                     /gene="yjaH"
                     /locus_tag="ACHKA1_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO5680485.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(8555..8827)
                     /gene="hupA"
                     /locus_tag="ACHKA1_002862"
     CDS             complement(8555..8827)
                     /gene="hupA"
                     /locus_tag="ACHKA1_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO5680486.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            complement(9014..9604)
                     /gene="yjaG"
                     /locus_tag="ACHKA1_002863"
     CDS             complement(9014..9604)
                     /gene="yjaG"
                     /locus_tag="ACHKA1_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO5680487.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            complement(9647..10318)
                     /gene="nfi"
                     /locus_tag="ACHKA1_002864"
     CDS             complement(9647..10318)
                     /gene="nfi"
                     /locus_tag="ACHKA1_002864"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO5680488.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            complement(10328..11392)
                     /gene="hemE"
                     /locus_tag="ACHKA1_002865"
     CDS             complement(10328..11392)
                     /gene="hemE"
                     /locus_tag="ACHKA1_002865"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO5680489.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGDVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            complement(11432..12205)
                     /gene="nudC"
                     /locus_tag="ACHKA1_002866"
     CDS             complement(11432..12205)
                     /gene="nudC"
                     /locus_tag="ACHKA1_002866"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO5680490.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            12300..12776
                     /gene="rsd"
                     /locus_tag="ACHKA1_002867"
     CDS             12300..12776
                     /gene="rsd"
                     /locus_tag="ACHKA1_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO5680491.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     regulatory      12828..12928
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13009..14904
                     /gene="thiC"
                     /locus_tag="ACHKA1_002868"
     CDS             13009..14904
                     /gene="thiC"
                     /locus_tag="ACHKA1_002868"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO5680492.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGAHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     gene            14904..15539
                     /gene="thiE"
                     /locus_tag="ACHKA1_002869"
     CDS             14904..15539
                     /gene="thiE"
                     /locus_tag="ACHKA1_002869"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO5680493.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQTADWRLATAQLLEIAGVGDE"
     gene            15532..16287
                     /gene="thiF"
                     /locus_tag="ACHKA1_002870"
                     /pseudo
     CDS             15532..16287
                     /gene="thiF"
                     /locus_tag="ACHKA1_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
     gene            16271..16471
                     /gene="thiS"
                     /locus_tag="ACHKA1_002871"
     CDS             16271..16471
                     /gene="thiS"
                     /locus_tag="ACHKA1_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO5680494.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINHQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            16473..17243
                     /gene="thiG"
                     /locus_tag="ACHKA1_002872"
     CDS             16473..17243
                     /gene="thiG"
                     /locus_tag="ACHKA1_002872"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO5680495.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            17240..18373
                     /gene="thiH"
                     /locus_tag="ACHKA1_002873"
     CDS             17240..18373
                     /gene="thiH"
                     /locus_tag="ACHKA1_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO5680496.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(18783..19321)
                     /gene="yjaZ"
                     /locus_tag="ACHKA1_002874"
                     /pseudo
     CDS             complement(18783..19321)
                     /gene="yjaZ"
                     /locus_tag="ACHKA1_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
     gene            complement(19534..23757)
                     /gene="rpoC"
                     /locus_tag="ACHKA1_002875"
     CDS             complement(19534..23757)
                     /gene="rpoC"
                     /locus_tag="ACHKA1_002875"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO5680497.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(23834..27862)
                     /gene="rpoB"
                     /locus_tag="ACHKA1_002876"
     CDS             complement(23834..27862)
                     /gene="rpoB"
                     /locus_tag="ACHKA1_002876"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO5680498.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            complement(28182..28547)
                     /gene="rplL"
                     /locus_tag="ACHKA1_002877"
     CDS             complement(28182..28547)
                     /gene="rplL"
                     /locus_tag="ACHKA1_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO5680499.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            complement(28614..29111)
                     /gene="rplJ"
                     /locus_tag="ACHKA1_002878"
     CDS             complement(28614..29111)
                     /gene="rplJ"
                     /locus_tag="ACHKA1_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO5680500.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            complement(29403..30107)
                     /gene="rplA"
                     /locus_tag="ACHKA1_002879"
     CDS             complement(29403..30107)
                     /gene="rplA"
                     /locus_tag="ACHKA1_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO5680501.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            complement(30111..30539)
                     /gene="rplK"
                     /locus_tag="ACHKA1_002880"
     CDS             complement(30111..30539)
                     /gene="rplK"
                     /locus_tag="ACHKA1_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO5680502.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            complement(30698..31243)
                     /gene="nusG"
                     /locus_tag="ACHKA1_002881"
     CDS             complement(30698..31243)
                     /gene="nusG"
                     /locus_tag="ACHKA1_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO5680503.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            complement(31245..31628)
                     /gene="secE"
                     /locus_tag="ACHKA1_002882"
     CDS             complement(31245..31628)
                     /gene="secE"
                     /locus_tag="ACHKA1_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO5680504.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
BASE COUNT         7352 a         8749 c         8223 g         7548 t
ORIGIN      
        1 ggttcttcgc gttgcatcga attaaaccac atgctccacc gcttgtgcgg gcccccgtca
       61 attcatttga gttttaacct tgcggccgta ctccccaggc ggtcgactta acgcgttagc
      121 tccggaagcc acgcctcaag ggcacaacct ccaagtcgac atcgtttacg gcgtggacta
      181 ccagggtatc taatcctgtt tgctccccac gctttcgcac ctgagcgtca gtcttcgtcc
      241 agggggccgc cttcgccacc ggtattcctc cagatctcta cgcatttcac cgctacacct
      301 ggaattctac ccccctctac gagactcaag cttgccagta tcagatgcag ttcccaggtt
      361 gagcccgggg atttcacatc tgacttaaca aaccgcctgc gtgcgcttta cgcccagtaa
      421 ttccgattaa cgcttgcacc ctccgtatta ccgcggctgc tggcacggag ttagccggtg
      481 cttcttctgc gggtaacgtc aatgagcaaa ggtattaact ttactccctt cctccccgct
      541 gaaagtactt tacaacccga aggccttctt catacacgcg gcatggctgc atcaggcttg
      601 cgcccattgt gcaatattcc ccactgctgc ctcccgtagg agtctggacc gtgtctcagt
      661 tccagtgtgg ctggtcatcc tctcagacca gctagggatc gtcgcctagg tgagccgtta
      721 ccccacctac tagctaatcc catctgggca catccgatgg caagaggccc gaaggtcccc
      781 ctctttggtc ttgtgacgtt atgcggtatt agctaccgtt tccagtagtt atccccctcc
      841 atcaggcagt ttcccagaca ttactcaccc gtccgccact cgtcagcaaa gaagcaagct
      901 tcttcctgtt accgttcgac ttgcatgtgt taggcctgcc gccagcgttc aatctgagcc
      961 atgatcaaac tcttcaattt aaaagtttga tgctcaaaga attaaacttc gtaatgaatt
     1021 acgtgttcac tcttgagact tggtattcat ttttcgtctt gcgacgttaa gaatccgtat
     1081 cttcgagtgc ccacacagat tgtctgataa attgttaaag agcagttgcg acgcggcttt
     1141 cagcccactg tcgcgaggtg gcgtatatta cgctttcctc tttcagagtc aaccctgaat
     1201 ttcagggttt ttctcttcaa ccgaaccggc tgtttgtgtg aagtgattca catccgccgt
     1261 gtcgatggag gcgcattata gggagttctc cgcaggtcgc aatagaaaaa ttgcataaaa
     1321 ataactgact gctgcattcc ccagcaaaac cccgctttat acctttttac acacagagtt
     1381 atccacaatc atcagcgtta tttttgtatt tttcacacgg taaccactgt caaaattgtg
     1441 atcaccattg aaagagaaaa attcgcgagc gttgcgcaaa cgttttcgtt acaatgcggg
     1501 cgaaaaataa ggatgccccg ttaggggcgt tagctgagtt tttcgcgaaa aattcagcta
     1561 acgctctctg taatagtcaa atccagggga tttaccatgc aacaacgtcg tccagtccgc
     1621 cgcgctctgc tcagtgtttc tgacaaagcc ggtatcgtcg aattcgccca ggcactttcc
     1681 gcacgcggtg tggagctgct gtctacaggg ggcactgccc gtctgttagc agaaaaaggt
     1741 ctgccggtaa ccgaagtttc cgattacacc ggtttcccgg agatgatgga tggacgcgtg
     1801 aagaccctgc atccgaaagt acatggtggc attctgggcc gtcgcggcca ggacgatgcc
     1861 attatggaag aacatcagat ccagcctatc gatatggtgg ttgttaacct gtatccgttc
     1921 gcccagacag tggcccgtga aggttgctcg ctggaagatg cggttgagaa catcgatatc
     1981 ggcggcccaa cgatggtgcg ctccgccgcc aagaaccata aagatgtcgc aatcgtggtg
     2041 aagagcagcg actacgacgc cattattaaa gagatggatg acaacgaagg atcgctgacg
     2101 cttgcaaccc gtttcgacct cgccatcaaa gccttcgaac acactgccgc ctacgacagc
     2161 atgattgcca actacttcgg cagcatggtt ccggcttacc acggtgaaag caaagaagcc
     2221 gccggtcgct tcccgcgcac gctgaacctg aacttcatta agaagcagga tatgcgctac
     2281 ggcgagaaca gccaccagca ggctgccttc tatatagaag agaatgtgaa agaagcctcc
     2341 gttgctaccg caacccaggt tcagggtaaa gccctctctt ataacaacat cgccgatacc
     2401 gatgcggcgc tggagtgcgt gaaagagttc gccgagccgg catgtgtgat tgtgaagcac
     2461 gccaaccctt gcggcgtggc tatcggcaat tccattcttg atgcttacga tcgcgcgtac
     2521 aaaaccgacc cgacctccgc attcggcggc atcattgcct ttaaccgcga gctggatgcg
     2581 gaaaccgcac aggccatcat ttctcgtcag tttgttgaag tgattattgc gccgtccgcc
     2641 agcgaagaag ccctgaaaat caccgccgcc aaacagaatg tacgcgttct gacctgcggt
     2701 cagtggggcg agcgtgttcc gggcctcgat ttcaaacgcg tgaacggcgg tctgctggtt
     2761 caggatcgcg atctgggcat ggtcggtgcg gaagaactgc gcgtcgtcac ccaacgtcag
     2821 ccgaccgaac aggaactgcg tgatgcgctg ttctgctgga aagtggcgaa gttcgtgaaa
     2881 tccaatgcta tcgtctatgc caaaaacaat atgaccatcg gtattggcgc gggccagatg
     2941 agccgcgtgt actccgcgaa aatcgccggt attaaagctg ccgatgaagg cctggaagtg
     3001 aaaggttcct cgatggcttc tgacgcgttc ttcccgttcc gcgacggtat tgatgccgcc
     3061 gcggctgcgg gcgtgacctg tgtaatccag cctggcggtt ctatccgtga tgacgaagtg
     3121 attgccgccg ccgacgagca cggtattgcg atgctcttca ccgacatgcg ccacttccgc
     3181 cattaatgga gcaatagatg aaagtattag tgattggtaa cggcgggcgc gagcacgcgc
     3241 tggcctggaa agcggcccag tcgccgctgg ttgagactgt ttttgttgct ccgggtaatg
     3301 caggcactgc gctggaaccc gcgctgcaaa acgttgctat cggcgtgacc gatatcccgg
     3361 cgctgttgga tttcgcacaa aacgaaaaga ttgatctgac catcgtcggc ccggaagcgc
     3421 cgctggtgaa aggcgtggtc gataccttcc gcgctgccgg gctgaaaatc ttcggcccaa
     3481 ccgcaggtgc ggcccaactg gaaggctcaa aagcgtttac caaagatttc ctggcccgcc
     3541 ataagatccc tacggcggaa taccagaact tcaccgaggt agaacctgcg ctggcgtatc
     3601 tgcgtgagaa aggcgcgcca atcgtcatta aagcggacgg tctggctgcc gggaaaggcg
     3661 ttatcgtggc gatgacgctg gaagaagcgg aagcggctgt tcacgatatg ctggcgggca
     3721 acgcttttgg cgacgcgggt catcgcatcg ttatcgaaga gttcctcgat ggcgaagaag
     3781 cgagctttat cgtgatggtg gacggcgagc atgtgctgcc gatggctacc agccaggatc
     3841 acaaacgcgt aggcgataaa gataccggac caaacacggg cgggatgggc gcttactccc
     3901 ccgcgccggt agtaaccgat gaagttcatc agcgcaccat ggaacgtatt atctggccaa
     3961 ccgtgaaagg catggcggcg gaaggcaaca cctacaccgg ttttctctac gcgggcctga
     4021 tgatcgacaa acagggcaat ccgaaggtta ttgaatttaa ctgccgcttt ggcgatccgg
     4081 aaacccagcc gattatgctg cgcatgaagt ccgatctggt tgagctctgc ctggcggcct
     4141 gtgaaggcaa actggacgag aaaacgtcag agtgggatga acgtgcttct ctcggcgtgg
     4201 tgatggctgc gggtggatat ccgggcgatt accgcaccgg cgatgtgatc cacggcctgc
     4261 cgctggaaga agtggctgac ggcaaagtgt tccacgcggg cacgaaactg gcggataacg
     4321 agcaggtagt gaccagcggc gggcgcgtac tgtgcgtcac cgcgctgggt cataccgtgg
     4381 cagaagcgca gaaacgcgct tatgccttaa tgaccgatat tcactgggac gactgcttct
     4441 gccggaaaga tatcggctgg cgcgctatcg aacgcgagca gaactaacgc gacagttttg
     4501 ccaatagcgt tttgcgcgtg atccctaact gacgggcggc ttcggttttg ttgccgcccg
     4561 ttttctccag tgccgccaga atcacttctt tttcgacttc caccagcggc tgaatatcct
     4621 gactttgccc cagcgggatc ggcgtaccgg caatcgccag cggcagctcg cgttcggaaa
     4681 tatattcccc ggtcagcagc accaccgccc gttccaccgc gttttccagt tcacgaatat
     4741 ttcccggcca gtcgtagtgg atcagcaaat ccatcgcctg tggcgtaaag ccttttaccg
     4801 ccttgcgatt acgctcggca aagcgctgaa gaaaatggcc agccagcaga gggatatctt
     4861 cccgccgttg ccgcagcgat ggtacttcaa tcgccaccac attcaggcga tagtagagat
     4921 cctggcgaaa acgcccggca ttcacctctg cggcaagatc gcgatgggtc gccgcaatca
     4981 gccggacatc aaccgagata gtctggttgc taccgacacg ctgaacttcg cgctcctgaa
     5041 tcgcacgcag cagacgcacc tgcatcatcg gcgagatatc gccaatttca tcgagaaaca
     5101 gcgtgccgcc gtccgcctca acaaagcgcc cttcccggcg tttatcggct ccggtaaacg
     5161 cccctttttc gtgaccgaac aattcagatt ccagcaagga ttcgttaagt gccgcacagt
     5221 tgagcgttac cagcggtttt tcgctacgtg cgctactggc gtgaatcgcc ctggcgacca
     5281 gctctttacc ggtgccggaa tcgccgtgga tcagcaccgt ggcttccgat ggcgcgacga
     5341 gggcgatttc actgagcagg tgttgcatcg ccgggctttt accgaccata ccgaactggc
     5401 tggcagtcac cgcaggtgtt tcagcatcaa tactgtgcgt atgcgcgagc gccttttcca
     5461 gcgtcgcctg taggttatcg aaatccagcg gcttgatgag ataatccagc gccccagttt
     5521 tcagtgcctc taccgccgtc tcgacgctgg agtacgcagt cataatcagc accggaattg
     5581 ccgggtttaa cgctttgatc tctttcagcg tggcgatgcc gtccatctcc gccattcgca
     5641 catcgcaaag cacaagatca aaaacctgtt cccgcacctg ctccagcgcc tgtcgcccgc
     5701 tgttcgccag cgcgacgtta tagccccagc cgcgcagtaa agcctgcaaa atagtgcagt
     5761 ggctaatgtc atcatccacc accagaatat cgatattatc gtgcgtcatc cttgtgggtc
     5821 cttacgcgta atattgaccg gaagccagag ggtgaacgtt gagccttttc cctcctggct
     5881 tgcgacctga attgtaccac cgtgttgttc aacaatatta tgcacgaccg ccagccccag
     5941 tccggtgcct tcggctttag tggtgaagta cggagtgaag atggcatcaa gctgatctgc
     6001 cgcaattccc ttaccgctgt cggtaacgct gattttcacg cccgcgccgc tttcgctggc
     6061 cgtcacgcta atcacgccat gctgaccaat cgcctgaata gcattgagat agagattcaa
     6121 caggacctga gtcagcctgt ccggatcggc ctgaatttcc ggtaatgtgt cgttggcggt
     6181 aaagcgtaac tggatctccc ggctgtttgc atcctggctt accagctgta atgagtggtt
     6241 aatcagcgtg ttgagatcca ccgcctgcaa agccagatgc gttggcttaa ccagttccag
     6301 caactcgctt accacgcggt ttaaacggtc ggcctctttc gccatcacct gcgccagttg
     6361 atgcgcttct ccccctgcag gcgcgcgctc ggcaaagtat tttgccagtc ctttaatcga
     6421 ggaaagtggg ttacggattt cgtgggcaac gcctgccgca agatgcccca gcgccaccag
     6481 cttctctttg cgcttcattt catcttgtag aagctggcgc gagcgcagat agcggcgata
     6541 ccagaagaat gacaatacgc ttgccagcaa gaccgtcgcc agggcgaaga ggataatcag
     6601 ggtgtttctc tgctcacgat cttcggcact cacaatatta ctggcgtcga aagcgatgaa
     6661 aattgcttgt ggtgtcgcgg catactgctg catatggcgc atccggtgca ttccagcagc
     6721 aaacatcggt tgaaactggc gataaatttc cagaacaggc tcaccgtctg cgctatcgat
     6781 ccgccgccac gcttcttcat ctcccggatg taactgctgc atttcctgcg gggaataaag
     6841 ctgttttccc accatgccgg agttgctatg catcacgatt gttccttgtt catccgtgac
     6901 cgcaaaccaa cgtactccag gctgcccggc catttcttcc agtaaggcct gctgctgcgc
     6961 atgatgcatg cgcatcccca tgccgacgcg cgagccggat tcgagagcac gaataagtac
     7021 actgcctttt tccagcagcg tttgtctggc agcggcagtc tcgcgcccat aatcgcgaat
     7081 caccgtcacc gcaaacaacc ctaccagccc aacaatgacc acggggagga tcgcgcttaa
     7141 ccatttagct aaggagtctt tagaacgttg cataaaacgc atcttcttct ttgcctgctc
     7201 atcccttttt gacagcagaa atcatgccat cttttatcag cgcttaccct gcgctgtaac
     7261 acaaaggctt aagtttcaat gagtaaaaat gactcgctac ccgcagcagg cgagtcattt
     7321 ttactcgttt atcatgccag attacccgtc atatcagcgt ttcatcgttg gcacggaaga
     7381 tgcaataccc gaagtaagac aaccactgga ggattaacca tgaaacggaa cacgaaaatt
     7441 gccctggtga tgatggcgct ttcagcaatg gcgatgggat cgacatctgc atttgctcac
     7501 ggcggacacg gtatgtggca gcaaaatgcc gcgcctttga ccagcgaaca acagacagcg
     7561 tggcagaaaa tccataatga cttttacgct caaagcagcg cactgcaaca gcaactagtg
     7621 acgaagcgtt atgaatacaa tgccctgtta gccgcgaacc caccggatag cagcaaaatt
     7681 aacgcggtcg ccaaagagat ggagaatttg cgtcagtcgt tagatgagtt acgggtgaaa
     7741 cgggatattg cgatggctga agcgggtatt ccgcgcggag ccggaatggg catgggctac
     7801 ggcggctgcg gtggtggcgg tcatatgggt atgggccact ggtaaatcag aacgttttct
     7861 cgttgggttg ccagcgacag aaatcagagt ttgccactaa cagtaattgc gaaccttcgg
     7921 gcgcttccag ccatgccacg ctgacatcaa cagaagagcg gctctggcgg cgctcgatat
     7981 gcgctatcgc ctgcgaatct aaatcaggtt taatggtttg cccttcgcag gtggtcacgg
     8041 tctgttccat cgcatgccag cgcccctggc gcagcacgac tctcccctga cgtaaggcat
     8101 cgctaatctg gcggatctga tcagcccgat aacggtataa atcgatttgg tcgttagaaa
     8161 gctgctgctt ttccccatcg gattcacgtt gcataaagct cagatcaccg cgatcgtcaa
     8221 aacgcgcgcg aatgttcacc ggcggtttgc tgtaaacatt gatattgatg agcgtgaggt
     8281 tatcgccctg ccagcgatac tctcgcgtcg tggtatcgcc actgcgccat gggctaaaca
     8341 cggcaagcag atgcacatta tcgccgctgt cttttcgcca gatgcgtacc gcgccctggt
     8401 cttcagcaaa tccactggcg gtaaagggag gaagtgacga gttgtgacta caggcagtga
     8461 gaaacagcgc acctgccagc atcaatgatc gacgccagaa agacaaaagg ggtgaaacca
     8521 ccccttcgtt aaaactgttc actgccacgc aatcttactt aactgcgtct ttcagtgcct
     8581 tgccagaaac aaatgccggt acgttagctg cggcaatttt gatttcttta ccggtctgcg
     8641 ggttgcggcc agtacgctca gcgcggtggt tcactttgaa ggtaccgaaa ccaaccagtt
     8701 gtacagcatc gccttctttc agagactcag taattgcagc cagagtggac tccagagcag
     8761 ctttagcctg ggttttggac agttctgctt tctctgcaat tacatcaatc agttgagtct
     8821 tgttcataag ttatccttac aatgtgttta tcgcttgcta agcatcgagt gcgacggaaa
     8881 tgccagaata gcactctcct gcatacacgc accgatagcc actttttttc gccccccaaa
     8941 tgtagaccag acggggggcg aaagggaaga ctccaggtat gacaaatcag gcgttaaatc
     9001 acgttttctg gtcttattgc tgaaaaatta taccaatatt gctctcaccc gcctcacgca
     9061 ggtctgccct aaggcctttt atcagctcga tatcgcgttc ttcgcactca gctaaaagtc
     9121 ggaatatttc ccactgaatg tcccattctt gctctacagc tgggttttct ttgagctctt
     9181 catcgctcat ttcgcgacca gcctgagtca tttccagcat cgcaacggtc gtgatggagg
     9241 tcttactcac ttccaccgcg tgttcgagcg tttcaccact caaacgcgaa tggaccagtt
     9301 cacttaacgc cacgcaggca tcgattgccg gataaacgcc gtacagatcg aaatcgtcgg
     9361 ctgaaggaat cgcttcttca aatttctcca gttggctgtc gaaatttact tttgcgtctt
     9421 tgacggtcag cgtttcccag atgagatcga gaatacggcg gtaaatttgc ccatcgccaa
     9481 aaccggtttg ctggcagaac atggcgtaat tggggtacat gcgttcgcat aagcaagcca
     9541 tgaaagtgac atgctgccag ctttccaggc gctccagacg cagatgaatt gggttttgta
     9601 acatgttgag ttctcaaata cggaaattac ccgcagttta cctgaattag ggctgatttg
     9661 ctgtatagcg cacgaacgct ggacgttccg aggccaccgc gtccgcccag cgcgttggct
     9721 ccggcagacg atagcctttc atgcagcgtt gtacccacgc cagcgcgctg tccacgctga
     9781 cccgatggcc ggtagcgata aacaacgggt tacagcgcgc tttgctgcgc cagacccagg
     9841 ccagctgctc gcctttatcc atcagtgggg ccagcgcgcc cggttcgctg gagagcggtt
     9901 cgaatttacc gcatagtcgt tttttcgcta cgccaatggt cggcacatcc accagcaaac
     9961 caaaatggct ggcgacgcca agacggcgag gatgcgagat cccatgacca tcgacaaaca
    10021 ctaaatccgg tttttgtgac agcatctccc acgctgccag gagcgcagga tattcgcgga
    10081 aggaaagaaa acctggaatg taaggcatgg tggtagcgat gcgggccact ttatactcca
    10141 ccagctcaag cgaggggtat ttcagcagca ccatcgccgc tcgcgtcact tctccgccct
    10201 gctcaaaccc gacatcggct ccggcgatca gatccggcgg atccttatcg agtcgatcct
    10261 cgcggatcac agaagaagcc agttcgattt gttgagcgcg taatgacgcg agatccataa
    10321 tcgctcctta gcggtgatac tgttcagaca gtcgatgcac tgcctccacg aacacgccag
    10381 catgttctgg cggcacatcc tgatgaatgc cgtgaccaag gttaaagaca tgaccttcgc
    10441 cgtgaccgaa acctgcaagt atagtcgcta cttcttcttc aatgcgggca ggcggcgcgt
    10501 acagcatcga cggatccata ttaccctgca acgcgacttt attgcccaca cggcggcgcg
    10561 catcggcgat gtccgttgtc cagtcgaggc ccagcgcatc gcaaccggtt tctgccatgg
    10621 cttccagcca ctgtccgccg cctttggtaa acagcgtgac cggtacgcgg cgaccgtcgt
    10681 tttcacgcag taaaccatca acaattttat gcatgtaata aagcgagaac tgttgataat
    10741 cgcgcccggt aagcacgccg ccccaggtgt cgaaaatcat cacagcctga gcaccggctt
    10801 taatctgcgc attcagatac aaagtgacgc ttttcgccag tttatcgagt agagcgtgca
    10861 gcgcctgcgg atcggcatac atcatttttt tgatcacggt gaaggctttg ctgctgccgc
    10921 cttccaccat gtaggtcgcc agcgtccacg ggctgccgga aaaaccaatc agcgggacgt
    10981 cgcctttcag ttcgcggcga atggtacgca ccgcgttcat cacgtaaccc agctcatctt
    11041 ccgggtccgg aattggcagt ttatcgacgt cagctttgca ggtgactggc gaggtaaaac
    11101 gcggaccttc tccggcctca aaatagagcc ctaaccccat cgcgtccggc acggtgagga
    11161 tatcggaaaa gaggatcgcc gcatccagcg ggtagcgacg cagcggttgc aaagtcactt
    11221 cgcacgccag ctcggcgttt ttgcacagcg acataaaatc gcctgcctgg gcgcgcgtgg
    11281 ctttatattc cggtagatag cgacccgcct ggcgcatcat ccatactgga gtgacatcaa
    11341 cgggctggcg cagcagcgcc cgcagataac gatcgttttt aagttcggtc attttggctg
    11401 ttccttagtg cgtcaggcgg tcagtgtatc atcactcgta ctctgcccga cacatcgcca
    11461 ccgtatcttc tatcagacgg cgcgccacgg tgccgggcgg cgggagtaac ggcaaatcgt
    11521 catagcgata ccagttcgcc tcgagcaatt ctttcgggtc gatcacgatg tcgccgctgt
    11581 catattccgc cataaacgcg gtcattaaag actgaggaaa cggccacggc tgggaagtca
    11641 cataacgcaa gtttttaact ttaattccgc tctcttccat cacttcccgc gcgaccgcct
    11701 gctcgagggt ttcgcccact tcgacgaatc cggcaagtac cgtatggaca ccgttacgat
    11761 ggcgggtatg ctgggcgagg aggatcgaat catcgcgacg gatggcaaca ataatgcagg
    11821 gggcgatttg cgggtagtaa cgctcacggc aatggctgca cagcatcgcc cattcggttt
    11881 tgcttggata catttcatgc ccgcagtaac cacagtattt atgcgatcgg taaaactccg
    11941 ccagttgcac gccacgtccg gccagttgaa acagcccaac atcgagatca atgacctgac
    12001 gtaccgaccc catatcgtga cgccgctgct gttgtattaa ccaaacaggt tccccctgcc
    12061 attcgccgat ttgtagtgcg cgctgaccca caagatcgaa atttgccgct tcgccatatg
    12121 gcaattctcc cttcggcaac cataattttt gttcatggct gacgacccac cagccgtgat
    12181 ctaatttttc aattatacga tccatagctc ttgcactacc tttgcatcac tggcatgttt
    12241 aacatggttt ttacatttct cactgagcag tttttgaata caaacttgcg gagtcaatca
    12301 tgcttaacca gctcgataac ctgacggagc gcgtcagagg aagtaacaaa ctggttgatc
    12361 gctggctaca tgtacgtaag catctgctcg tggcttacta caatctggtt ggcattaagc
    12421 ctggcaaaga atcgtacatg aggctaaacg aaaaagccct tgatgatttt tgtcagagcc
    12481 tggtcgatta cttgtctgcc ggacatttca gtatttatga gcgcattctt cataagctgg
    12541 aaggcaacgg gcaactcgcg cgcgccgcga agatttggcc gcaactcgaa gccaataccc
    12601 aacagattat ggattactac gattccagcc tggaaaccgc tatcgatcat gataattacc
    12661 ttgagtttca acaggtttta tctgacatcg gcgaggcgct ggaagcgcgc tttgtgctgg
    12721 aagataagct gattctgctg gtgcttgacg ccgcccgcgt caaacatcct gcttgagttc
    12781 tgcgctgtta acgcgtaatt tacattcaac gccccgtttg tagggctaat ttcttgtcgg
    12841 agtgccttaa ctggctgaga ccgtttattc gggatccgcg gaacctgatc aggctaatac
    12901 ctgcgaaggg aacaagagtt aatctgctat cgcatcgcct ttgcggcgat cgtctcttgc
    12961 ttcatccgtc gtctgacaag ccacgtcctt aactttttgg aatgagctat gtctgcaaca
    13021 aaactgaccc gccgcgaaca acgcgcccag gcccaacatt ttatcgacac cctggaaggc
    13081 accgcctttc ccaactcaaa acgcatttac atcactggcg cacaccccgg cgtgcgcgtg
    13141 ccgatgcgtg agatccagct tagcccgacg ctaatcggcg gcagcaaaga acagccgcag
    13201 tacgaagaaa acgaagcgat tccggtctac gacacctccg gcccgtatgg cgatccgcag
    13261 atcgccatta acgtgcaaca agggctggca aaactacgcc agccgtggat cgatgcgcgc
    13321 ggcgataccg aagaacttac cgtgcgcagt tccgattaca ctaaagcgcg gctggcagat
    13381 gatggcctcg acgagctgcg ttttagcggc gtattaacgc caaaacgcgc caaagcagga
    13441 cgccgcgtca cacaactgca ctacgcccgc cagggcatca tcacaccgga aatggaattc
    13501 atcgccatcc gcgagaatat gggccgcgag cgcatccgta gcgaagtttt acgccaccag
    13561 catccgggaa tgagctttgg cgcacgtctg ccggaaaata tcactgcgga atttgtccgt
    13621 gatgaagttg ctgccggacg tgcgattatc ccggccaaca ttaatcatcc ggaatcggag
    13681 ccgatgatta ttggccgtaa cttcctggta aaagttaacg ccaatatcgg caactcagcg
    13741 gtcacctctt ccatcgaaga agaagtggaa aagctggtat ggtccacgcg ctggggagcg
    13801 gatacggtga tggatctctc caccggtcgc tatattcacg aaacccgcga gtggattttg
    13861 cgtaacagcc cggtgccgat cggtacagtg ccgatctacc aggcgctgga gaaggttaac
    13921 gggatcgccg aagatcttac ctgggaagcg ttccgcgaca cgctgctgga acaggccgag
    13981 caaggtgtgg attacttcac tatccatgcg ggggtgctgc tgcgctatgt gccgatgacc
    14041 gcgaaacgcc tgaccggtat cgtctcgcgc ggcggttcga ttatggcgaa atggtgcctc
    14101 tcccatcatc aggaaaattt cctctatcaa cacttccgcg aaatttgtga aatctgtgcc
    14161 gcttatgacg tttcgctgtc gctgggcgac ggtctgcgcc ccggttctat tcaggacgcc
    14221 aacgatgaag cacagtttgc cgagctgcat acgctgggcg aactgaccaa aattgcctgg
    14281 gaatatgacg tgcaggtgat gattgaaggc ccaggccacg tgccgatgca gatgatccgc
    14341 cgcaatatga ccgaggagtt agagcactgc cacgaagcgc cgttttacac tctggggccg
    14401 ctaactaccg atattgcgcc gggctatgac cacttcacgt cggggattgg tgcggcgatg
    14461 attggctggt ttggctgcgc gatgctctgt tacgtaacgc caaaagagca tctgggtctg
    14521 cctaataaag aagatgttaa gcaggggctt atcacctata agattgccgc ccacgccgct
    14581 gacctggcga aagggcatcc gggcgcgcaa attcgcgata acgccatgtc gaaagcccgc
    14641 ttcgaatttc gctgggaaga ccagtttaat ctggccctcg acccgtttac cgcccgcgct
    14701 tatcacgatg aaaccctgcc ccaagagtcc ggcaaagtcg cccatttttg ctccatgtgt
    14761 ggaccgaaat tttgctcgat gaaaatcagc caggaagtgc gtgattacgc cgccacgcaa
    14821 actattgaaa tgggaatggc ggatatgtcg gagaacttcc gtgccagagg cggagaaatc
    14881 tacctgcgta aggaggaagc gtgatgtatc agcctgattt tcctcctgta ccttttcgtt
    14941 taggactgta cccggtggtg gacagcgtac agtggatcga acgtctgttg gatgcaggcg
    15001 tacgtactct ccagctacgc atcaaagatc ggcgcgatga agaggtggaa gccgatgtcg
    15061 tggcggcaat tgcgctgggc cgccgctata acgcgcgatt gtttatcaac gattactggc
    15121 ggctggcgat caagcatcag gcgtatggcg tccatttggg gcaggaagat ttgcaagcca
    15181 ccgatctcaa tgccatccgc gcggcaggcc tgcggctggg cgtttcgaca catgacgata
    15241 tggaaatcga cgtcgcgctg gcagcacgcc cctcttatat cgcgctggga catgtgttcc
    15301 cgacgcaaac caaacagatg ccttctgcac cgcaggggct ggaacagctg gcacggcatg
    15361 ttgagcgcct ggcggattat cccaccgtgg cgattggcgg tatcagtctg gcgcgtgcgc
    15421 ctgcggtgat agcaacgggt gtcggcagta tcgccgtcgt cagcgccatt actcaaaccg
    15481 cagactggcg tttggcaacg gcacagttgc tggaaattgc aggagttggc gatgaatgac
    15541 cgtgatttta tgcgttatag ccgccaaatc ctgctcgacg atatcgctct tgacgggcag
    15601 caaaaactgc tcgacagcca ggtgctgatt atcggtcttg gcgggctggg tacacctgct
    15661 gcgctatacc tggcgggcgc tggcgtcggg acgctggtac tggcagatga cgacgatgtg
    15721 catttaagca atctgcaacg acaaatcctc tttaccactg aagatatcga tcgcccgaaa
    15781 tcgcaggtca gccatcagcg actgacacag ttgaatcccg atattcaact gacagcatta
    15841 caacaacggt taacgggtga ggcgttaaaa gatgcggttg cacaagccga tgtggtgctc
    15901 gactgtaccg acaatatggc gactcgccag gagattaatg ccgcctgcgt ggcactcaac
    15961 acgccgctta tcaccgccag cgcggtcgga tttggcggtc agttgatggt actgacgccg
    16021 ccctaggagc aggggtgtta ccgctgcctg tggccagata accaggagcc agaacgcaac
    16081 tgccgcacgg cgggcgtggt tggcccggtg gtcggggtta tgggcacttt gcaggcactg
    16141 gaagccatta agttattaag cggtatagag acgcctgcgg gagaactccg actgttcgac
    16201 ggtaagtcga gccagtggcg cagcctggcg ttgcgccgcg ccagtggttg cccggtatgc
    16261 ggaggaagca atgcagatcc tgtttaacga tcaaccgatg cagtgcgccg ccgggcaaac
    16321 tgttcacgaa ctactggagc aactcgacca acgacaagcg ggcgcggctc tggcgattaa
    16381 tcatcaaatc gtcccgcgtg agcagtggac gcaacatatc gtgcaggatg gcgaccagat
    16441 cctgcttttt caggttattg cagggggttg agatgttacg tattgcggac aaaacgtttg
    16501 attcacatct gtttaccggc accgggaaat tcgcttcttc acaactgatg gtggaggcga
    16561 tccgcgcttc cggcagccag ctggtgacac tggcgatgaa acgtgtcgac ttgcgccagc
    16621 acaacgacgc tatcctcgaa ccgcttatcg cggcgggtgt gaccctgctg ccaaatacat
    16681 ccggggcgaa aacagcggaa gaagccattt tcgccgccca tctggctcgt gaagcgttag
    16741 gcacaaactg gttaaaatta gagattcacc ctgacgcccg ctggctgttg cccgatccca
    16801 tcgaaaccct gaaagccgcc gaaacgctgg tacaacaggg atttgtcgtg ctgccttact
    16861 gcggggccga tccggtattg tgtaaacgtc tggaagaagt cggctgtgca gcggtgatgc
    16921 cgctcggcgc gccgattggc tcgaatcagg ggctggaaac ccgcgccatg ctggagatta
    16981 ttatccagca ggccacagtt ccggtggttg tcgatgctgg catcggcgtt cccagccatg
    17041 ccgcgcaggc gctggaaatg ggggccgacg cggtgttagt gaatacggcg attgccgtcg
    17101 cggacgatcc cgtcaacatg gcgaaggcat ttcgtctggc agtagaagca ggcctgctgg
    17161 cacgtcagtc cggaccgggc agccgcagtc attttgctca tgccaccagc ccgctgaccg
    17221 gatttctgga ggcatcggca tgaaaacctt cagcaatcgc tggcgacaac tggactggga
    17281 cgacatccgc ctgcgtatca acggcaaaac ggctgctgac gtagagcggg cgctaaatgc
    17341 ctcgcaactc acccgcgacg acatgatggc gctgttatcg cctgccgcca gtggctatct
    17401 ggaacaactg gcccaacggg cacagcgtct gacccgtcag cgatttggca acacagttag
    17461 tttctacgtc ccgctttatc tttccaatct ttgcgctaac gactgcacgt actgcggatt
    17521 ttccatgagt aatcgcatca agcgcaaaac gctggatgaa gcggatattg ccagggaaag
    17581 cgccgctata cgggagatgg gctttgaaca tctgctatta gtcactggtg aacatcaggc
    17641 gaaagtgggg atggattact ttcgtcgtca tctccctgcc ctgcgtgaac agttctcttc
    17701 actacagatg gaagtgcaac cgctggcgga gacggaatac gccgagttaa agcaacttgg
    17761 tctggatggg gtgatggttt atcaggagac atatcacgag gcgacatatg cccgccatca
    17821 tctgaaaggc aaaaaacagg acttcttctg gcggctggaa acgccggatc ggctgggccg
    17881 tgcggggatt gataagatag gcctcggcgc gctaattggc ctttccgaca gctggcgcgt
    17941 tgactgctat atggttgcag aacatttgct atggctgcaa cagcattact ggcaaagccg
    18001 ttactctgtc tcctttccgc gcctgcgccc gtgtactggc ggcattgagc ctgcgtcgat
    18061 tatggatgaa cgccagttag tgcaaaccat ctgcgccttc cgactgcttg caccggagat
    18121 tgaactgtca ctctccacgc gggaatcacc gtggtttcgc gatcgcgtta ttccgctggc
    18181 gatcaataac gtcagcgcct tctcgaaaac gcagccaggt ggctatgctg ataatcaccc
    18241 cgagttggaa cagttctcac cgcacgacga tcgcagaccg gaagcggttg ctgcagcgtt
    18301 aaccgctcag ggtttgcagc cggtatggaa agactgggac agctatctgg gacgcgcctc
    18361 gcaaagacta tgacacggaa tgtaagaacg taaatttatt ccgagccgcc acggaagatg
    18421 aaaaacgcaa ggttgttgaa agcgttgcgt ttttttatgg tagtgccgtc agcaggaatg
    18481 ttgtccaggg cgaaaagctt cttcctcgtt tcgccctgcc tttcttaaaa attccgtaat
    18541 gcaaagtctg aagccatcgc ctgttgagca atgaaagaga tctgattgta agagagtaaa
    18601 tactcaacta tgatagagac gaaaataaga acacatgttc tcatcttcca ggatgcagca
    18661 gactgaagaa attcagacat cccgcaacct gcgattatcg caaggtcaag gcaaagtccg
    18721 gtaatggcgt tctgaatacc agagataatt ctctggcgaa acccacctta aggtgggttt
    18781 tgttattttg agggctgagg aagctgctta tttctcaata agttgtaggc aatggtgaaa
    18841 aatttcactg atttaccttt ctgtttttca gaaatgggat aagcgctagt tacatgcaaa
    18901 cgaagttttt tcttttccct gaaagccctg aacgcaacaa agtaaaatgc cttgtctcct
    18961 ccgtctaagt ccacctcaat cacggcatag ctaccatacc cggcatgaat aacgttgctt
    19021 tctggcagcg ccaaaatagt tctttttaag tgtgtgcgcg ctaagttata acggtgctgg
    19081 cagaagggac gagattcttt aggcgcgtgg tacattaacg attgtttttc ttcgttcgtc
    19141 tgatgttcat aatcttttgt gaagcagtga agagagtagg taacccaaaa gcgatactct
    19201 aaaggctctt tgccctcatg atgctgaacg tagacagcct gatgagcatc aagaaatgat
    19261 aaatcacgat atcaccatcc ggatgtccaa aaggtcgcca cttttccact tcctgtttca
    19321 ttctcgttcc ttaagaaagt tacacaggaa tagtatcgaa tccttgccga gtttgactca
    19381 attagttcag tcagtttcag gatattagtc atctctacat tgattatgag tatccagaaa
    19441 ttcattaaat attctgacaa atgctctttc cctaaactcc ccccataaaa aaacccgccg
    19501 aagcgggttt ttacgttatt tgcggattaa cgattactcg ttatcagaac cgcccagacc
    19561 tgcgttcagc agttctgcca ggctggcaga tgcgtcttct gcagtcacct gcggtgcagc
    19621 cggagcttca cccgcagcac ggcgacgcat acgatcctgg tggtacgcgt aaccggtacc
    19681 tgccgggatc agacgaccca cgataacgtt ctctttcagg ccgcgcagtt cgtcgcgttt
    19741 gcccgcaacg gctgcttcgg tcagcacgcg agtggtctcc tggaacgatg ccgcggagat
    19801 gaaggactcg gttgccagag acgctttggt gatacccagc agatcgcggg agtaagttgc
    19861 acccactttg ccgttcgctt ccagttcgcg gtttgcgatc ttgacgcgag agtattcaac
    19921 ctgttcgcct tccaggaagt cggagctgcc cgcattaacg atggtagctt tacgcagcat
    19981 ctgacgaacg ataacttcga tgtgtttatc gttaatctta acgccctgca gacggtatac
    20041 gtcctgtact tcgttaacga tgtaacgagt cacagcatga acaccacgca gacgcagaat
    20101 gtcgtgcggc gcttccggac cgtcggaaat tacgtcacca cgttctacac gttcaccttc
    20161 gaacacgttg agctgacgcc atttcggaat catctcttcg tacggatcgc taccgtctac
    20221 cggggtgata accagacgac gtttaccttt ggtttcttta ccgaaggaaa cgataccgct
    20281 gatttcagcc aggattgccg gctctttcgg acgacgtgct tcgaacaggt ccgcaacgcg
    20341 tggcagacca ccggtgatgt ccttggtacc gccggattcc tgcggaatac gcgccagggt
    20401 gtcaccagag ctgatctgta cgccatcttc cagctgaaca atcgctttac ccggcaggaa
    20461 gtactgcgca ggcatatcgg tacctgggat cagaacgtcg ttaccctgag catcaacgat
    20521 tttcagtgcc ggacgcagat ctttaccacc tgcggtacgt tctgcggaat ccagaaccac
    20581 cagcgaagac agaccggtca gttcgtcggt ctgacgagta atggtctggc cgtcgatcat
    20641 gtcagtaaag cgtacaaaac cgcttacttc ggtgataacc ggcatggtgt gcgggtccca
    20701 gtttgcaacg gtttcgccgc cagcaacctg ttcgccatcg cctttcgcca gtaccgcacc
    20761 gtaaggtact ttgtagcttt ctttagtacg accgaattcg tcgatcagtt tcagttcggt
    20821 gttacgggaa gtgataacca gtttaccgct ggagttcaca accgacttca cgttgctgag
    20881 cttgatgcta cctttgtttt tcacctggat gctggattca gcagccgcac gagatgccgc
    20941 accaccgatg tggaacgtac gcatggtcag ctgtgtaccc ggttcaccga tggactgtgc
    21001 cgcgataaca ccgattgctt cacccttgtt gatgatgtgg ccacgcgcca ggtcacgacc
    21061 gtagcagtgc gcacatacac caaagtcggt gtcacaagat acaacagaac gtactttaac
    21121 cgcgtcgaca gagttctctt ccagcaggtc acaccactgt tcgtgcagca gcgtgttgcg
    21181 cggaacgagg atatcagcag tacccggctt cagaacgtct tcagcagtta cacgacccag
    21241 tacgcgatcg cgcagcggct ctttaacgtc accaccctcg ataaccggag tcatcatgat
    21301 accttcatgg gtaccacaat cgtcttcggt aaccaccagg tcctgcgcca cgtcaaccag
    21361 acgacgagtc aggtaaccgg agttcgcagt tttcagtgcg gtatccgcca gacctttacg
    21421 agcaccgtgg gtggagatga agtactggag tacgttcaga ccttcacgga agttcgcggt
    21481 gattggcgtt tcgatgatgg agccatccgg cttcgccatc agaccacgca taccagcaag
    21541 ctgacgaatc tgtgccgcag aaccacgcgc accggagtcg gccatcatgt agatgctgtt
    21601 gaaggaaacc tgcttctctt cctgaccgtc acggttaata acggtttcag tttgcaggtt
    21661 atccatcatc gctttggata cacgatcgtt cgccgcagcc cagatatcga taactttgtt
    21721 gtagcgttcg cccgcagtta ccagaccaga ctggaactgc tcctgaattt cagcaacttc
    21781 tgcttctgcc tcggagatga tttcgtgttt cttctccggg atgaccatgt catcgatacc
    21841 aacagatgca ccagaacgcg ctgcatatgc aaagccggtg tacatgatct ggtccgcaaa
    21901 aataacggtc ggtttcagac cgagaatgcg gtagcaggtg ttcagcattt tggagattgc
    21961 ttttttaccc agcgcctggt tgacgatgga gtaaggcaga cctttcggta caatcatcca
    22021 cagaatggca cggccaacag tcgtgtcttt caggctggtt ttcgctacta attcaccgtt
    22081 agcatctttt tcatactcgg tgatacgcac tttaacgcgc gcatgcagag aagccagacc
    22141 agagcgatac agacgttctg cttctttcgg gccagtcagc accatgcctt cgcctttggc
    22201 gttaacacag tcacgggtca tgtagtacag acccagtaca acgtcctgag acggaacgat
    22261 gattggttcg ccgttcgccg gggacaggat gttgttggta gacatcatca gcgcacgcgc
    22321 ttccagctgg gcttccagcg tcagcggtac gtgaacagcc atctggtcac catcgaagtc
    22381 ggcgttatat gccgcacaaa ccagcgggtg cagctggata gctttacctt cgatcagtac
    22441 cggttcaaat gcctggatac ccagacggtg cagagtcggt gcacggttca gcagtaccgg
    22501 gtgttcgcgg ataacttcgt ccaggatatc ccaaacgaca gcttcttcgc gctcaaccat
    22561 tttcttcgca gctttaatgg tggtagcaag accacgcagt tcaagcttgc cgtagatgaa
    22621 cggtttgaac agctccagcg ccattttctt cggcagaccg cactgatgca gacgcaggta
    22681 tggacctacg gtgattacag aacgaccgga gtagtcaaca cgcttaccga gcaggttctg
    22741 acggaaacga ccctgtttac ctttgatcat gtcggccaaa gatttcagag gacgcttgtt
    22801 agaaccggtg atcgcacgac cgcgacgacc gttatccagc agggcgtcta ccgcttcctg
    22861 cagcatacgt ttttcgttac gtacgatgat gtccggcgca gccagatcca gcagacgttt
    22921 cagacggttg ttacggttaa tgacgcgacg atacagatcg ttcaggtcag aagtcgcgaa
    22981 acgaccacca tccagcggaa ccagcggacg cagatctggc ggcagtaccg gcagaacggt
    23041 caggatcatc cactctggtt tgttaccaga ctgaacgaac gcttccagca gtttgatacg
    23101 cttggtcagc tttttacgct tagtttcgga gttggtttcg ttcagctctt cacgcagctg
    23161 ttcgcactct tgctccagat ccatgctctt cagcagagcc tggattgctt ccgcacccat
    23221 tttcgcgtcg aattcgtcac cgaactcttc cagcgcgtcc agatactgct cttcagtcag
    23281 gatctgctga cgttccaggt tggtcatacc gccttcgata accacatagg attcgaagta
    23341 cagtacgcgt tcaatatcgc gcagcggcat atcgagcagc aggccgatac gggacggcag
    23401 cgatttcagg aaccagatgt gcgcagtcgg ggaagccagt tcgatgtggc ccatacgctc
    23461 acggcgtact ttagtctggg tcacttcaac gccacacttc tcacagatga cgccacggtg
    23521 tttcaggcgc ttgtacttac cgcacaggca ctcgtaatct tttaccggcc caaagatacg
    23581 ggcgcagaaa aggccgtcac gttctggttt gaacgtacgg tagttgatgg tttccggctt
    23641 tttaacttca ccgaaagacc atgaacggat catgtctggc gaagccagag caattttgat
    23701 cgcatcaaac tcttcggttt tagtctgcgc tttcagaaac tttaataaat ctttcacgga
    23761 tttgctcccg tcggagttag cacaatccgc tgccgggttt taccccgaca gcagtgacct
    23821 gtttgagcga gaattactcg tcttccagtt cgatgttgat acccagcgaa cgaatctctt
    23881 tcaacaatac gttgaaggat tctggcatgc ccggctccat ctgatggttg ccgtccacga
    23941 tgtttttata catcttagta cgaccattca cgtcatcaga cttaacggtg agcatttcct
    24001 gcagggtgta tgctgcgccg tatgcttcca gcgcccacac ttccatctcc ccgaaacgct
    24061 gaccaccgaa ctgtgcctta ccacccagcg gctgctgagt aaccaggctg taagaaccgg
    24121 tggaacgcgc gtgcatcttg tcgtcgacca ggtggttcag tttcagcatg tacatgtaac
    24181 caacggttac cggacgctcg aactgttcac cagtgcgacc atcgtacagg cggatctgac
    24241 cggaagtcgg caggtcgcca agtttcagca gctctttaat ttctgcttct ttcgcaccgt
    24301 cgaacaccgg cgttgcgatt ggcatacctt tgcgcaggtt ttcagccaga cgcataactt
    24361 cttcatcgct gaaggtactc aggtcaactt tctgacgaac gtcagcgccc agatcgtacg
    24421 cacgctggat gaattcgcgc agtttcgcga cttcttgctg ctgtttcagc atggcgttga
    24481 tcttgtcgcc gatacctttc gcagccatac ccaggtgggt ttcgaggatc tgaccgatgt
    24541 tcatacgaga cggtacgccc agcgggttca gtacgatgtc taccggcgta ccgttttcat
    24601 cgtaaggcat atcttcgatc gggttgatct tagaaattac acccttgtta ccgtgacgac
    24661 ctgccatctt gtcaccaggc tggatacggc gtttaaccgc cagatatacc ttaacaatct
    24721 tcagcacgcc cggtgccaga tcgtcgccct gggtgatttt gcggcgtttc gcttcgagtt
    24781 tcttctcgaa ctcgtgtttc agttcgtcat actgctcagc cagctgttcc agctgatttt
    24841 gtttctcttc gtcggtcagg cccagctcca gccagcgatc gcgcggcagt ttgtcgagct
    24901 tctcagcttc aacgccaccg gctaccagca cagcacggat acggctgaac agacccgctt
    24961 cgaggatctg cagttcttca gacaggtctt tcttcgcctg tttgagctgc atttcttcga
    25021 tttccagcgc acgtttgtct ttttctacgc catcgcgagt aaagacctga acgtcgataa
    25081 ccgtaccgga tacaccgttt ggtacgcgca gagaagagtc tttaacgtca gacgctttct
    25141 caccgaagat cgcacgcagc agtttttctt ctggggtcag ctgagtttca cctttcggcg
    25201 taaccttacc aaccagaatg tcgccaccgg tcacttccgc accaatataa acgataccgg
    25261 attcatccag tttggagagc gcagcttcac ccacgttcgg gatgtcagcg gtgatctctt
    25321 ccggccccag cttggtgtca cgggacacac acgccagttc ctgaatgtgg atggtggtga
    25381 aacggtcttc ctgaacaaca cgctcggata cgaggatgga gtcttcgaag ttgtaaccat
    25441 tccacggcat gaacgctacg cgcatgttct gaccaagcgc cagttcaccg aggtcggtgg
    25501 acggaccgtc tgccagcacg tcgccacgtt caaccggttc acccagagac acacacggca
    25561 tctggttgat acaggtgttc tggttagaac gggtgtattt ggtcaggttg tagatgtcga
    25621 tacctgcttc acccggatac atctcgtctt cgttaacttt gataacgata cgggaagcat
    25681 ccacgtactg aacgacacca ccacgtttag caaccgcagt tacaccggag tcaacggcaa
    25741 cagcacgttc cataccagta ccaaccagcg gcttatcagc acgcagagtc ggaacggcct
    25801 gacgttgcat gttcgcaccc atcaatgcac ggttggcgtc atcgtgttcc aggaacggga
    25861 tcagggacgc accgacggat accacctgct gggtggatac gtccatgtag tcaacctggt
    25921 cacggctgaa caagctggat tcgcctttgc tacggcaggt taccaggtct tctacgaagt
    25981 ggccttcttc atccaggttg gagttcgcct gggcgataac gtagttgcct tcttcgatag
    26041 cagacaggta gtgaatttcg tcagttacaa caccgtcggt cactttacga tacggagtct
    26101 caaggaagcc gtattcgtta gtctgtgcgt acacggacag agagttgatc agaccgatgt
    26161 tcggaccttc aggggtttcg attggacata cgcgaccgta gtgagtcggg tgtacgtctc
    26221 gaacttcgaa gcctgcacgt tcacgggtca gaccgcctgg gccgagtgcg gagatacgac
    26281 gtttgtgcgt aatctcagac agcgggttgt tctggtccat aaactgagac agctggctgg
    26341 aaccgaagaa ctctttcact gctgcggaaa tcggcttggc gttgatcata tcctgaggca
    26401 tcagggtatc cagatcgccc agagacagac gctctttcac cgcacgctct acacgtacca
    26461 ggccaacgcg gaactggttt tccgccattt cgccaacgga acggatacga cggttgccga
    26521 ggtggtcgat atcatcgact tcgcctttac cgttacggat atcgatgagc tttttcataa
    26581 catcaatgat gtcgtctttg ctcaggatac cagaaccttc gatttcttcg cgcagcagag
    26641 aacggttgaa cttcatacga ccaaccgcag acaggtcata acggtcttcg gagaagaaca
    26701 ggttctcgaa caggctttcc gctgcttcac gagtcggcgg ctcgccaggg cgcatcatgc
    26761 ggtagatttc taccagtgcg ctcagacggt cgttagttgg gtcgacacgt aaggtttcag
    26821 agatatatgg gccgtgatcc agatcattgg tgaacagcgt ttcgatacgc ttgtgaccag
    26881 actggctcag cttagccagc agatccaggc tcagctccat gttcgctgcg cagatcagct
    26941 cgccggtaga ctcatcaata tagtctttag caaccacttt acctgcgatg tactcaaccg
    27001 ggacttcgat cagtttgacg tcgtcttttt ccagctggcg aatgtggcgc gcagtgatac
    27061 ggcggccttt ttctacgtac actttaccgt tagcttcgat gtcgaaagat gcggtttcac
    27121 cacgcaggcg ttccggcacc agttccatct gcagcttgtt atcacggatt tcaaagataa
    27181 ctttttcaaa gaacaggtcg aggatctgct ctgtggtgta gttcagggca cgcagaatga
    27241 tggtcgcagg cagtttacgg cgacggtcga tacgtacgaa caggttgtcc ttcggatcga
    27301 attcgaagtc cagccaggaa ccacggtaag ggatgatgcg cgcgttatac agcactttac
    27361 ccgaagagtg ggttttacct ttgtcggagt caaagaagac gcccggacta cggtgcagct
    27421 gggaaacgat aacacgctca gtaccgttga taacaaaggt accgttgtct gtcatgagcg
    27481 gaatttcgcc catgtagact tcttgttctt taatgtcttt tacggtgcct tccggcgctt
    27541 cgcgctcata gatcaccaga cgcagtttaa cgcgcagcgg tgcggaatag gtcacgccac
    27601 ggatttgaca ttcctggacg tcaaacaccg gttcgccaag gcggtagctg acgtattgca
    27661 gctcggaatt accgctgtag ctctgaatcg ggaatacgga acggaaagca gcttccagac
    27721 catactgccc ttcaggatct tgctcgataa atttctgaaa cgagtcaagc tggatagaaa
    27781 ggagataagg tacatccaga acttgtggac gtttaccaaa atccttacga atacgttttt
    27841 tctcggtata ggagtaaacc atagggttcc tcagctcgct gacaagtcga cccatctgtc
    27901 cattgagcgg acagtttgtg caacactatt ttgttgaccg gaaaatggaa cactttccgc
    27961 aatgcctgtt gctatcacgc ttaaaccatt tcattgcgat ttacacagaa cggacgtcct
    28021 gtcgcagtat attaagtcgt cgatagaaac aagcattgaa aggcacagca gtagtcaaac
    28081 agtgtgaaac gctactggcg ccttacagcg caaaaaggct ggtgactaaa aagtcaccag
    28141 ccatcagcct gatttctcag gctgcaaccg gaagggttgg cttatttaac ttcaacttca
    28201 gcgccagctt cttccagagc ttttttcagt gcttctgcgt cgtctttgct cacgccttct
    28261 ttcagagcag ccggtgcaga ttctaccagg tctttagctt ctttcagacc caggccagtt
    28321 gcgccacgta ctgctttgat aacagcaact ttgttagcgc cagcagcttt cagaattacg
    28381 tcgaattcag ttttttcttc agcagcttca accgggccag cagctacagc tacagcagca
    28441 gcagcggaaa caccgaattt ttcttccatt gcagagatca gttctacaac gtccattaca
    28501 gacatagctg caactgcttc aatgatttga tctttagtga tagacattta aattgttcct
    28561 gaatatcaga ataagtttat acgtaagcga atgcgttaaa aagataactg cgattaagca
    28621 gcttctttcg catcgcgtac agcagccaga gtacgaacca gtttgccagc cgaagcttct
    28681 ttcatggttg ccatcaggcg tgcaattgct tcttcgtagg tcggcagagt tgccaggcgg
    28741 tcgatctgag acgccgggat cagctcacct tcaaaggcag cggctttgac ctcaaatttt
    28801 gcattcgctt tcgcgaactc tttgaacaga cgagcagcag cgcccgggtg ttccatagag
    28861 tatgcaatca gggtcggacc aacaaacgcg tctttcaggc actcgaacgg agtaccttca
    28921 acagcgcggc gcagcagggt gttacgaaca acacgcatgt atacgccagc ttcgcgacct
    28981 gctttacgca gttcagtcat tttatctaca gttacgccac gggaatccgc aactactgca
    29041 gacagcgcgc ctttggctac ttcgctgact tcagcaacaa tcgcttgttt gtcttgaaga
    29101 tttaaagcca ttagctttgc tcctggatgt ttgccagaga aaatctctgg aactcacttc
    29161 actcctccaa acggagagag cgtcttaatt acggtgagca gaaacaagcc agaatataaa
    29221 agaataatct tagcgttctg tcaccgtcta cgcaggggat taagtttctt gtgaaactcc
    29281 tgcggtcttc gacggaggcc tggataggcc aggctccaac gaacaaatct ttttagctgc
    29341 taactttcgt tagcatacgt gggggtaaga ttgtagacaa aatcaccgcc cacgtaaagg
    29401 cattagttta cagaagcgct caggccagcc tggtcaactg caacacctgc acccatggtg
    29461 gtggagatgc taactttctt gatgtacacg cctttcgcct gagtcggttt tgcttttttc
    29521 agcgcaacca gcagagcttc caggttttct ttcagtttgt cagcgtcaaa gtccacttta
    29581 ccgatggtgg tgtggatgat gccgtttttg tcgttacggt aacgaacctg gccagcttta
    29641 gcgtttttaa ccgcttcagc aacgttcggc gttacagtac ccactttcgg gtttggcatc
    29701 aggccgcgcg gacccagaac ctggcccagc tggccaacaa cgcgcattgc atccggagaa
    29761 gcaataacaa cgtcaaagtt catttcgcct ttcttgatct ggtcagccag atcttccata
    29821 cctaccagtt cagcacctgc agctttagca gcttcagcgt ttgcaccctg ggtaaatacg
    29881 gctacgcgaa cggaacggcc agtaccgtgc ggcagtacag ttgcaccacg tacgttctgg
    29941 tcagatttac gagcgtcgat gccgaggtta acagcaacgt ccacgctttc tacgaattta
    30001 gcagtcgcca gctctttcag cagagcgata gcttcgttga tgtcgtactg tttagttgca
    30061 tcaactttct cgcggataac acgcatgcgc ttggtcagtt tagccatttc ttagtcctcc
    30121 actaccaggc ccatggaacg tgcagtacct tcgatggagc gagtcatcgc ttcaatgtcg
    30181 gcaccagtca tgtcggcagc tttggtctgc gcgatttcct gcagctgagc gcgggaaatt
    30241 ttacccactt tgtctttgtt cggcttaccg gaaccagact tgataccagc cgcttttttc
    30301 agcagaactg ctgccggcgg agtcttggta acgaaagtga aagaacggtc agcgtaaacg
    30361 gtgattacta ccggaatcgg cagacctttt tcgatggaat cagtttttgc gttgaacgct
    30421 ttgcagaatt ccatgatgtt tacgccctgc tgacccagag ccggacctac tggcggactc
    30481 gggttagcca taccagctgc aacctgcagc ttgacatagg cttgtacttt cttagccatt
    30541 ataaattcct caagttgggt aataacgcct ctgagaggct ccccgtgata taaaaaatcg
    30601 ttttacgggc aaggcccata aaaacaaaag gcgcgaaatt gtattccaat ctcgcgcctt
    30661 gtgcaacgat taaatcgccg cttttttgat cgctgggtta ggctttttca acctggctga
    30721 agtccagctc taccggggtc gcacgaccga agatagaaac agacactttc agacgagatt
    30781 tctcgtaatc cacttcttca acaacaccgt tgaagtcagc gaacggacca tcattaacac
    30841 ggaccatttc acccggttca aacagcgttt tcggacgcgg cttatcacca acctgctgca
    30901 ggcggttcat aatcgcatcg acttctttat cgctgattgg cgcaggacga tcggaagtac
    30961 cgccgatgaa gcccatcaca cgcggtacgc tgcgcaccag gtgccagctc gcgtcgttca
    31021 tcaccatctg aacgaggacg tagccaggga agaatttacg ttcgcttttg cgacgctgac
    31081 cgccacggat ttcaaccact tcttcggttg gtaccatgac ttcaccaaac aaatcttcca
    31141 tgttgtgtaa tttgatatgc tcacgcagcg acgttgctac gcggccttca aaaccggaaa
    31201 acgcctgaac gacgtaccag cgctttttag gagcttcaga catctcagaa cctcaggcca
    31261 gtgataaagg ataccaggcg aaccagaata ccatccagtc cccacaggat cagtgacatt
    31321 actgcggtaa ccgcagccac aatcagcgtg gtgtgcaatg tttcctggcg agtcggccaa
    31381 atgaccttac ggacttcggt acgcgcttca cgggcaaaag caacggtagc tttacctttt
    31441 gtcgttaaca gcgcgacacc acccgctgca gcaatcagaa ttactacggc cagcgcacgc
    31501 agcggcagca taatgtcgcg ataaaggtag ttgccgacaa tcgccaccag gagcaatgcc
    31561 accacaacga cccacttcat cgcttccagg ccgcgcccgc ttccttgagc ttcggtattc
    31621 gcactcataa accaacctgt cagaagtatt ctacaaacat tttcaccccg cgatcgcgag
    31681 gcaaaccaaa tcgaaacgcg tattagcttt tcggattata cgccctcaac agagcctgtc
    31741 tcagcaatga ttatgacaaa gaaaatcact gatgagccag gttctggtac gaaagcgtgc
    31801 aaaaagggca tcaaatgatg cccttttagt gcgcattgcg tcaaatgtta tcggcaatta
    31861 gctcagaact tt
//
LOCUS       DAZFKF010000053        31607 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFKF010000053 DAZFKF010000000
VERSION     DAZFKF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.053"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(138..2405)
                     /gene="adiA"
                     /locus_tag="ACHKA1_002883"
     CDS             complement(138..2405)
                     /gene="adiA"
                     /locus_tag="ACHKA1_002883"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO5680505.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2604..3512)
                     /gene="melR"
                     /locus_tag="ACHKA1_002884"
     CDS             complement(2604..3512)
                     /gene="melR"
                     /locus_tag="ACHKA1_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO5680506.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSRATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3795..5150
                     /gene="melA"
                     /locus_tag="ACHKA1_002885"
     CDS             3795..5150
                     /gene="melA"
                     /locus_tag="ACHKA1_002885"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO5680507.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLATYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5253..6674
                     /gene="melB"
                     /locus_tag="ACHKA1_002886"
     CDS             5253..6674
                     /gene="melB"
                     /locus_tag="ACHKA1_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO5680508.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKIPPEPVHADIPVGAVSDVKA"
     gene            complement(6813..7442)
                     /gene="yjdF"
                     /locus_tag="ACHKA1_002887"
     CDS             complement(6813..7442)
                     /gene="yjdF"
                     /locus_tag="ACHKA1_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO5680509.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     MPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLKLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7565..9211)
                     /gene="fumB"
                     /locus_tag="ACHKA1_002888"
     CDS             complement(7565..9211)
                     /gene="fumB"
                     /locus_tag="ACHKA1_002888"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5680510.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSNYISVADFNGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPEELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDSGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9289..10629)
                     /gene="dcuB"
                     /locus_tag="ACHKA1_002889"
     CDS             complement(9289..10629)
                     /gene="dcuB"
                     /locus_tag="ACHKA1_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO5680511.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11200..11919)
                     /gene="dcuR"
                     /locus_tag="ACHKA1_002890"
     CDS             complement(11200..11919)
                     /gene="dcuR"
                     /locus_tag="ACHKA1_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO5680512.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11916..13547)
                     /gene="dcuS"
                     /locus_tag="ACHKA1_002891"
     CDS             complement(11916..13547)
                     /gene="dcuS"
                     /locus_tag="ACHKA1_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO5680513.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13728..13958
                     /gene="yjdI"
                     /locus_tag="ACHKA1_002892"
     CDS             13728..13958
                     /gene="yjdI"
                     /locus_tag="ACHKA1_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO5680514.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            13970..14242
                     /gene="yjdJ"
                     /locus_tag="ACHKA1_002893"
     CDS             13970..14242
                     /gene="yjdJ"
                     /locus_tag="ACHKA1_002893"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5680515.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            14469..14765
                     /gene="ghoS"
                     /locus_tag="ACHKA1_002894"
     CDS             14469..14765
                     /gene="ghoS"
                     /locus_tag="ACHKA1_002894"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO5680516.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            14793..14966
                     /gene="ghoT"
                     /locus_tag="ACHKA1_002895"
     CDS             14793..14966
                     /gene="ghoT"
                     /locus_tag="ACHKA1_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO5680517.1"
                     /translation="MALFSKILIFYVIGVNISFIIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(15085..16602)
                     /gene="lysS"
                     /locus_tag="ACHKA1_002896"
     CDS             complement(15085..16602)
                     /gene="lysS"
                     /locus_tag="ACHKA1_002896"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO5680518.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(16839..18296)
                     /gene="dtpC"
                     /locus_tag="ACHKA1_002897"
     CDS             complement(16839..18296)
                     /gene="dtpC"
                     /locus_tag="ACHKA1_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO5680519.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMVQESND"
     gene            complement(18355..20502)
                     /gene="cadA"
                     /locus_tag="ACHKA1_002898"
     CDS             complement(18355..20502)
                     /gene="cadA"
                     /locus_tag="ACHKA1_002898"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO5680520.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(20582..21916)
                     /gene="cadB"
                     /locus_tag="ACHKA1_002899"
     CDS             complement(20582..21916)
                     /gene="cadB"
                     /locus_tag="ACHKA1_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO5680521.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(22282..23820)
                     /gene="cadC"
                     /locus_tag="ACHKA1_002900"
     CDS             complement(22282..23820)
                     /gene="cadC"
                     /locus_tag="ACHKA1_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO5680522.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(24080..24239)
                     /locus_tag="ACHKA1_002901"
                     /pseudo
     CDS             complement(24080..24239)
                     /locus_tag="ACHKA1_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(24703..25029)
                     /locus_tag="ACHKA1_002902"
     CDS             complement(24703..25029)
                     /locus_tag="ACHKA1_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5680523.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(25026..25289)
                     /locus_tag="ACHKA1_002903"
     CDS             complement(25026..25289)
                     /locus_tag="ACHKA1_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO5680524.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            complement(25361..26227)
                     /locus_tag="ACHKA1_002904"
     CDS             complement(25361..26227)
                     /locus_tag="ACHKA1_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5680525.1"
                     /translation="MSGITISRPEVVNGHTDVICSTSIGHILAVRKTTLMQIDTLIRQ
                     LTEISAMTESIGGKTAPDWAIKQNFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISAANILSTFEQLHQNKDEVFERGVINLFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHFITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITLRLDDHIQSVQGKENYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            complement(26312..26509)
                     /locus_tag="ACHKA1_002905"
     CDS             complement(26312..26509)
                     /locus_tag="ACHKA1_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5680526.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(26521..27009)
                     /locus_tag="ACHKA1_002906"
     CDS             complement(26521..27009)
                     /locus_tag="ACHKA1_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5680527.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGIELAELINKVAENGYS
                     LRVVDDSDQQSTCTPPPLTTLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKMFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     DW"
     gene            complement(27006..27383)
                     /locus_tag="ACHKA1_002907"
     CDS             complement(27006..27383)
                     /locus_tag="ACHKA1_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO5680528.1"
                     /translation="MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     NERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRMVNNITQGKHPEAQQ"
     gene            complement(27473..27841)
                     /locus_tag="ACHKA1_002908"
     CDS             complement(27473..27841)
                     /locus_tag="ACHKA1_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO5680529.1"
                     /translation="MSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRADIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCDAD
                     TLGSCGYVYIAIYPTPGTKT"
     gene            complement(27891..28535)
                     /locus_tag="ACHKA1_002909"
     CDS             complement(27891..28535)
                     /locus_tag="ACHKA1_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HFO5680530.1"
                     /translation="MSRIITSTVYTLNELSATAREKARDGYRQHHADSNWYENVYEDF
                     RKVCDIFGIDLRQRVFQLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQSATARIIR
                     EYAPQDRELHRIADALQAVQKRNFWQLQAEIHHRGRYCHPYSMDITVTRNSPTGQIMT
                     TDAEAAVSEALRDLAFWLYRQLENEYDWLTSDATVDEALLINEYTFTEAGLRAG"
     gene            complement(28554..28775)
                     /locus_tag="ACHKA1_002910"
     CDS             complement(28554..28775)
                     /locus_tag="ACHKA1_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO5680531.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(28838..29314)
                     /locus_tag="ACHKA1_002911"
     CDS             complement(28838..29314)
                     /locus_tag="ACHKA1_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO5680532.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(29330..29815)
                     /locus_tag="ACHKA1_002912"
     CDS             complement(29330..29815)
                     /locus_tag="ACHKA1_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5680533.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVASLVPDEQRISFWPQHF
                     GLIPQWVTLESRVFGWMDRLYEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNV
                     MNGNRAEMSPEAAGIAACLMAYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(29870..30688)
                     /locus_tag="ACHKA1_002913"
     CDS             complement(29870..30688)
                     /locus_tag="ACHKA1_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5680534.1"
                     /translation="MRLASRFGYANQIRRDHPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(30788..31021)
                     /locus_tag="ACHKA1_002914"
     CDS             complement(30788..31021)
                     /locus_tag="ACHKA1_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO5680535.1"
                     /translation="MPSYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            complement(31100..31555)
                     /locus_tag="ACHKA1_002915"
     CDS             complement(31100..31555)
                     /locus_tag="ACHKA1_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO5680536.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
BASE COUNT         7883 a         7550 c         7927 g         8247 t
ORIGIN      
        1 agcgcggggt ggggccgcgc aaaaagtatg acatttttgt ttgcagcaat ttttatgaaa
       61 acgttgccat actcaaaagg cctaaggcca ggcggcgtaa tgttatttaa acaattacgc
      121 cttcagcgga atagtggtta cgctttcaca cacataacgt ggtaaatacc gtcaataatt
      181 tcagtccctt cagtttcgtg ttcaaatcca gggaaatgat ggtcccagga ttgcagcgag
      241 cgtaaataac ttacttgcgg actgttttta tcgccgaagt tttcaccaga cagcagcatc
      301 gggattcctg gcggatacgg gataactgag tttgccgcga tacgtcctgg cagattttca
      361 atggatacca gttcgacatt gttgtcgaca atcgcgttgt acgcttcacg cggggtgatt
      421 tccgccaccg gtaggccgga ataggcttcg ttcaaccgtg cgccaggatt gttttctttc
      481 agccaggcaa acatggtgtc acccagatcg tgaatcccca tgttcgcgta agtgtcagga
      541 tattgttcaa caagttccgg catcacctgc gccagcggtg tgttggcgtc atagtggcgt
      601 ttgaaggagc aaagggtgtt aaccagagtc ccccatttcc cacgggttac gcccatagag
      661 aacaggaaca taatttggaa gtcagtggtg cgggtaggca caatgccgtg gcgaccaagc
      721 caggcagtga ccagcgccgc cggaacaccg gtttcttcca gttcaccatc ttcacccatt
      781 cccggagcaa ggatgctgac tttaatcggg tcgagcatac tccagttatc cggaatatct
      841 ttgaagccgt gccagctttc gcccggatgc attacccagc agtcctgaac ggtggtcaac
      901 agtttggttg gcgcgtcagc aaagtcatag gttttgccgg tttgtgggtc ggtgacgact
      961 tctttgttcc acggtttgaa gaaccagcta ccgtcagcgg tgaactcttt atacagccgc
     1021 gccatcgcct gacggaaatc aaccgcttcg tcgatcactt cctgcgtcag tgacaggcca
     1081 ctgttgccgt ccatcatcga caccgccacg tcgttggatg cgcagatggc atacagcggg
     1141 gaggtggtgg catgcatcat gtaggcctgg ttgaagcggg agaagttaat cgccccacga
     1201 ccttcacgta catgaatata agaggcctgt gacagcgcat tcagtagttt gtgggtggag
     1261 tgggtggcga aaacggtagg accgttgtga tcgccaggtt cgccgcgcat ggcatagtga
     1321 tcggcataga tcgggttgaa acgtgcatag ccgtaccagg cttcgtcaaa gtgcagacga
     1381 tcggaggttt tttccagcag atcctgcgct tctttagcgt tataacacac gccgtcatag
     1441 gtgcagttgg tcaccacgca gtaagacggt ttttgcccgg ctttgtcttt agtcagcggg
     1501 ctttcactga ttttcttctg caaggtttca ggttgcattt cctgcggata gattggcccg
     1561 ataatgccgt agcggttgcg gcttggcacc atgtagaccg gtttcgcgcc tgtcagcatc
     1621 aaaccttgtt cgatggattt atggcagtta cggtcaacga ccacgacatc gttatcggtc
     1681 atgcaagcct gcatgatggt gcggttagag ccggaagtac cgacgactac cgaccaggag
     1741 cgatcggcac caaatacgcg tgcggcatat ttttcgcttt cgccaaatgc gccagtatgg
     1801 tcaagcaaag aaccgaggga agttcgttcg atgcccatgt cggtgcggaa cagattttca
     1861 ccatagtagt catggtagaa acgtccggcg ggtgttttgg taaaaccaac gccgccctgg
     1921 tggcctggcg ctgcccagga atattcatgg atgtcgctat atttcatcag cgcgctgaac
     1981 agtggcggca acagctgctg gcggtagcgg gtcatcgcgg caacagcgcg tccggcgata
     2041 aagtcggcgg tatcttccag aatccaggcg aattcatcga caagctccag cagatcccgg
     2101 tccattgcgg cgagggcttt ttcccgatcg cccaacagga agaccggcac gttttgttgg
     2161 cgctcatgaa gcttaccgat caattgtctg acgttttgat gttcgtccgg atgttccatt
     2221 tgatagctga acatcaggca gtcaatggct tcgtttgaag agagaatggc aaaaccatca
     2281 tcaaaggagg tggatttaat cacggtaaca ttttgctggc ttaaagcatc tgccagacgc
     2341 tcaacagcgt taccgaccca ggtgtcttga tggagaaact cgctttcaac aattaatact
     2401 ttcatcattg cttacccggt tatgaaggaa tcttcgttgc gggcaagtat cttccggcct
     2461 tttcgggctg taaagcgcgt gaaaaacaaa cgtttttgga atatttgttt ttacaaacaa
     2521 tacgttatgt gattatttta aattattttc actgcttatt atcgacggct aaactatttt
     2581 tttggctgat actgatatcg tctttagccg ggaaacgtct ggcggcgctg ttggctaagt
     2641 ttgcggtatt gttgcggcga catgccgaca tatttgccga acgtgctgta aaaacgacta
     2701 cttgaacgaa agcctgccgt cagggcaata tcgagaatac ttttatcggt atcgctcagt
     2761 aacgcgcgaa cgtggttgat gcgcatcgcg gtaatgtact gtttcatcgt caattgcatg
     2821 acccgctgga atatccccat tgcatagttg gcattaagtt tgacgtgctc agccacatcg
     2881 ttgatggtca gcgcctgatc atagttttcg gcaataaagc ccagcatctg gctaacataa
     2941 aattgcgcat ggcgcgagac gctgtttttg tgtgtgcgcg aggttttatt gaccagaatc
     3001 ggttcccagc cagagaggct aaatcgcttg agcatcaggc caatttcatc aatggcgagc
     3061 tggcgaattt gctcattcgg actgtttaat tcctgctgcc agcggcgcac ttcaaacggg
     3121 ctaagctgct gtgtggctcg tgatttgatc accatgccgt gagtgacgtg gttaatcagg
     3181 tctttatcca gcggccagga gagaaacaga tgcatcggca gattaaaaat cgccatgctc
     3241 tgacaggttc cggtatctgt tagttggtgc ggtgtacagg cccagaacag cgtgatatgt
     3301 ccctgattga tattcacttt ttcattgttg atcaggtatt ccacatcgcc atcgaaaggc
     3361 acattcactt cgacctgacc atgccagtgg ctggtgggca tgatatgcgg tgcgcgaaac
     3421 tcaatctcca tccgctggta ttccgaatac agcgacagcg ggctgcgggt ctgtttttcg
     3481 tcgctgctgc acataaacgt atctgtattc atggatggct ctctttcctg gaatatcaga
     3541 attatggcag gagtgaggga ggatgactgc gagtgggagc acggttttca ccctcttccc
     3601 agaggggcga ggggagtgtc cgagcatcat gaggccgaaa actctgcttt tcaggtaaat
     3661 tattcccata aattcagatt tactgctgct tcacgcagga tctgagttta tgggaatgct
     3721 caacctggaa gccggaggtt ttctgcagat tcgcctgcca tgatgaagtt attcaagcaa
     3781 gccaggagat ctgcatgatg tctgcaccca aaattacatt tatcggcgct ggttcgacga
     3841 ttttcgttaa aaatattctt ggtgatgtgt tccatcgcga ggcgctgaaa acggcgcata
     3901 ttgccctgat ggacattgac cccacccgcc tggaagagtc gcatattgtg gtgcgtaagc
     3961 tgatggattc agcaggggcc agcggcaaaa tcacttgcca cacccaacag aaagaagcct
     4021 tagaggatgc cgattttgtc gtggtggcat ttcagattgg cggttatgaa ccttgcacgg
     4081 tgactgattt cgaggtctgt aagcggcatg gtctggaaca aaccattgcc gatacgttgg
     4141 ggccgggcgg tattatgcgc gcgctacgta ccattccgca tctgtggcaa atttgcgagg
     4201 acatgacgga agtctgcccc gatgccacca tgctcaacta tgttaaccca atggcgatga
     4261 atacctgggc gatgtatgcc cgctatccgc atatcaaaca ggtcgggctg tgccattcgg
     4321 tgcagggaac ggcggaagag ctggcgcgtg acctcaatat cgacccggct actctgcgtt
     4381 accgttgcgc aggtatcaac catatggcgt tttacctgga gctggagcgc aaaaccgccg
     4441 acggcagtta cgtgaatctc tacccggaac tgctggcgac ttatgaagca gggcaggcac
     4501 cgaagccgaa tattcatggc aatactcgct gccagaatat tgtgcgctat gaaatgttca
     4561 aaaagctggg ctacttcgtc acggaatcgt cagaacattt tgctgagtac acaccgtggt
     4621 ttattaagcc agggcgtgag gatttgattg agcgttataa agtaccgctg gatgagtacc
     4681 cgaaacgctg cgtcgagcag ctggcgaact ggcataaaga gctggaggag tataaaaacg
     4741 cctcccggat tgatattaaa ccgtcacggg aatatgccag cacaatcatg aacgctatct
     4801 ggacgggcga gccgagtgtg atttacggca acgtccgtaa cgatggtttg attgataacc
     4861 tgccacaagg atgttgcgtg gaagtggcct gtctggttga tgctaatggc attcagccga
     4921 ccaaagtcgg tacgctacct tcgcatctgg ccgccctgat gcaaaccaat atcaacgtac
     4981 agacgctgct gactgaagct attcttacgg aaaatcgcga ccgtgtttac cacgccgcga
     5041 tgatggaccc gcatactgcc gccgtgctgg gcattgacga aatatatgct cttgttgacg
     5101 acctgattgc cgcccacggc gactggctgc caggctggtt gcaccgttaa aacgcgacta
     5161 aacgctactg cgccggggga tttattccgg cgcacacctc tgacgatacc aataacagaa
     5221 ggcgggcgtt ggtaacagcg acccgatacc ctatgagcat ttcaatgact acaaaactca
     5281 gttatggatt tggagcgttc gggaaggatt ttgcgatcgg cattgtgtat atgtacctca
     5341 tgtattacta caccgatgtc gtcgggctgt ctgtgggttt ggtcggtact ttgtttctgg
     5401 tggcgaggat ctgggatgct attaacgatc cgattatggg atggattgta aatgctacac
     5461 gatcgcgatg gggtaagttc aaaccctgga tcctgatcgg tacgttggca aactctgtaa
     5521 tcttatttct cctctttagt gcgcatctgt ttgaaggtac tactcagatt gtctttgttt
     5581 gcgtgaccta catcctctgg ggcatgactt acaccattat ggatattccc ttctggtcgc
     5641 tggttccaac catcacgctc gataaacgtg agcgtgaaca actggttcct tatccgcgtt
     5701 tttttgccag tctggctggc tttgttacgg caggtgtgac gctaccattt gttaattatg
     5761 tcggcggtgg cgatcgggga tttggctttc agatgttcac actggtactg atcgcctttt
     5821 ttattgtttc aaccatcatc actctgcgca atgtgcatga agtcttttcg tcagacaatc
     5881 aaccgtctgc tgaaggaagc catctgacac ttaaagccat cgttgcgctt atttataaaa
     5941 acgatcagct ttcatgcctc ttgggtatgg ctcttgctta taatgtagcc agcaatatta
     6001 ttaccggctt tgctatctat tatttctcat atgttatcgg tgatgcggat ttgttcccct
     6061 attatctgtc gtatgcggga gctgctaacc tggtgacgtt agtattcttc ccacgcttag
     6121 ttaaatcatt atcccgacgc attttatggg ccggagcatc tattcttccg gtgttaagct
     6181 gtggtgttct cctgttaatg gcattaatga gctatcacaa cgtcgtcctc attgtgattg
     6241 cgggtatttt gctgaatgtg ggaacggcgc ttttctgggt attacaggtc atcatggtgg
     6301 cagataccgt tgattacggt gaatataaac tgcacgtacg ctgtgaaagc atcgcttact
     6361 ccgtgcagac tatggtggtg aagggcggtt cagcctttgc ggcttttttc attgcggttg
     6421 tgttagggat gattggctat gtaccgaatg ttgaacagtc tacgcaagcc ctattaggta
     6481 tgcagtttat tatgattgct ctaccgactc tgtttttcat ggtaacgctg attctctact
     6541 tccgtttcta tcgcctcaat ggcgacacgc tgcgcaggat ccagattcat ctgctggata
     6601 aatatcgcaa aataccgccc gagcctgttc atgctgatat tccggtcggt gcagtgagtg
     6661 atgtgaaagc ctgacgtgga caatagctaa cgaaaatggc ctgatgcgat atgtttatca
     6721 ggccacgtct taatggaatg ctttggattt gcaaattttg tagaccggat aaggaattca
     6781 cgccgcatcc ggcatcaaca aagtgcacgt tgttatccgg ttatcaagcc aaagcgccgt
     6841 agctggcggc aatgaaagcg agcgaggaat atcaccgtcg ttaatgcgcc aagtagcgcg
     6901 cagaacatat cggattgcgt gtcccactgg tcgccctggg tgccgagaaa atcatccgct
     6961 ccctggccca ttgccagcgc cgcccaccac tcgattaatt catacatcgc gcttatcgcc
     7021 agcgccacgc aacagaccag aaacgccacc attttacgtc cgcgaacgta catcccgcgc
     7081 acaaggatct ctcgtgccac cagtgcaggc accagcccct ggaaaaaatg ccccagcttg
     7141 tcatacggat tacggctcaa cttcagccat tcctgcacct caaaaccaac ggggactttc
     7201 gcgtaggtgt attgtccgcc gaccatcagg atgatggcgt gaaagaaaat gagcgtataa
     7261 agcagcggcg ttaacggata acgtttcgcg gtggcaagga gtagcggcat gacaataatc
     7321 accggtgtca cttccatcag ccaggtgagt ttgtcatggg ccgaaatgcc ggtataaatc
     7381 aggattaacg ttagcgccag tgcgccggtg ttaagaatta acggcttgag tgtgcgggtc
     7441 atggtgagtt cgcaagtcag ggaaaatact gactattcac cagcaagcgt gaaattacaa
     7501 tcgcaaaaga ggtggcccag ggggatcacc tggcagcatg ctgccaggcg ctgggccgaa
     7561 gaggttactt agtgcagttc gcgcactgtt tgttgacgat ttgctggaag aagtcgttac
     7621 ctttgtcatc gaccaggata aacgccggga aatcttctac ttcgattttc cagatagctt
     7681 ccatacccag ctccggataa gcgacgcatt ccagatgctt gatgctctgc tgcgccagta
     7741 ccgccgccgg accgccgatg ctaccgaggt agaagccgcc gtgtttatga cacgcgtcgg
     7801 taacctgctg actgcggtta cctttcgcta gcatgatcat gctgccgccg tgggattgca
     7861 gcagatccac ataggagtcc atacgacctg cggtggttgg gcctagtgaa cctgatggat
     7921 aaccggcagg ggttttcgcc ggacccgcgt agtagatcgg gtgatctttg atgtactgcg
     7981 gaagttcttt accggagtca atcagctctt tcagcttggc gtgtgcaata tctcgaccca
     8041 caataatggt gccggtgagc gacaaacgag tggataccgg gtattgcgaa agctgggcga
     8101 ggatctcttt catcgggcgg ttaaggtcaa ctttcaccgc ttcgccttca ccggcctggc
     8161 gcagttcttc cggaatgtac tggcctgggt tgtgttccag tttttcgatc cagatacctt
     8221 cgcggttgat tttcgcttta atgttacggt cagcggagca ggagacgccc atgcctaccg
     8281 ggcaggatgc gccgtgacgt ggcagacgga taacgcgaat gtcgtgcgcg aagtatttac
     8341 cgccaaactg cgcgccaaga ccgagtttct gggcctcttc cagcagttcc tgttccagct
     8401 ggacatcgcg gaacgcctga ccatgttcgt tcccttccgt cggcagttca tcgtaatagt
     8461 gagcgcttgc taacttgacg gttttcaggt tggtttccgc agacgtaccg ccaatcacaa
     8521 acgcgatatg gtacggcggg caggctgcag taccgagggt acgcattttc tcgacgagga
     8581 agtttttcag tttgccggga gtcagcaggg ctttggtttc ctggtagaga taggttttgt
     8641 tggcagagcc gccgcctttc gcaacgcaaa ggaatttgta ctcatcgcca tctaccgcgt
     8701 acaggtcgat ttgcgcaggc aggttagtac cggtgttgac ctctttgtac atgtccagcg
     8761 ccgcgttttg tgaatagcgc aggttatctt cgatataggt gttatagacg cctttcgaca
     8821 gcgcttcttc atcaccgccg ccggtccaca cgcgctgacc ttttttaccg acgatgattg
     8881 cggtgccggt atcctggcag gtcggcagca cgcctttggc ggcgatttcg gagtttctta
     8941 agaattgcag cgccacgtac ttgtcgtttt cgctggcttc tggatcgtga agaatagccg
     9001 caacctgttt ctggtgtgcc ggacggagca tgaaagaagc gtcgtgaaag gcttgctgcg
     9061 ccagcagggt cagggcttct ggttccactt tcaggatggt ttcgccgttg aagtcggcaa
     9121 cgctaatgta attggaagtg agtagatagt attcggtatt gtctttcccc atcgggaaag
     9181 gtgcctggta gataaagggt ttgtttgaca tagcttccag cctgtaactc tgtatttgtt
     9241 attcgaaaat ggcgtgccgc gtagtcacgg gcggcacgca aagtgcattt ataagaaccc
     9301 gtacatcgcg gcgaagatcc agccgaagac gcacgatacg ctcacaccaa tcaaccccgg
     9361 cagaataaag ctgtggttga tgacgaagcg accgatgtgg gtggtgccgg aacggtcaaa
     9421 ctgaatcgct gccagatcgc tcggataagt cggcaggatg taataaccgt agcaagccgg
     9481 tgctgaagcc acgatgtatg ccggatcaac gccgattgcc agcgcgaccg gaacaatcgc
     9541 cgccagcgcc gcagcctgag agtttacaaa cttggaaacc agcagcagaa caatggcata
     9601 ggcccacgga tactctttca ccatttcacc cagtacgccc tgaatttcag acatatgcgc
     9661 accgaacatg gtttctgcca tccatgcgat accgtacacc gccacgatgg cgatcatacc
     9721 ggaacggaag acttcgtttt ttgagataga cgcgggattg gttttggtca ggataataat
     9781 cagcgccccg gtcagcagca taaacatctg aataaccagt accatcgaca gcggtttgcc
     9841 gccgaaggat ggacgcaggt ccgaatcagc accaagaagt gcgactacag cgattgcccc
     9901 gaggaaaatc cacattgcca gccagttgct tttcggcagt tttttatcga gcagcgtcgc
     9961 ggtatcaccg taaacatact cacggttttc cggtacggag atgaatttct ggaactcttc
    10021 gtctttatcc agatctttac cgcggaacca gctgaagata ccgatcgcca ggataccgat
    10081 taacgtcgat ggaatggtga ttgccagcag gtcgaggaac tcaagatggc gaccatcaaa
    10141 ggtgacatta cccagcattg caaccagaga cacgaccgca accgacaccg gactggcgat
    10201 aatccccatc tgtgcaccga tagaacttgc cgccatcgga cgttccggac ggatgttgtt
    10261 cttaatggcg acgtcgtaga tgatcggcag aatggtgtaa accacatgac ccgtaccgca
    10321 aagaatggtc agtgtacagg tcacaaacgg cgcgacaatt gagacatatt tcgggttgcg
    10381 gcgcagcagc ttctcggcaa tttgcagcat gacatcaaga ccgcccgaag cttgcaaggt
    10441 cgccgatgcc gccaccaccg caatgataac cagcatgaca tcaaccggtg gtttacctgg
    10501 ctgaaggtgg aagacgaaga ccagaatgac cagaccgata ccgcctaata aacccagcgc
    10561 gataccaccc tttctggcac cataaaacag acatatcagt attatgataa gttggatagt
    10621 aaataacatg tgtgaaccct cgcgataatc ctatttaaat ttttgctgaa tagatcacag
    10681 tcacgttctg ttttgtatga actgttttca gagcctgact aaatatccgt ttggtcgtta
    10741 ccggctttag caaatacctc acagtgaata ttggctgatt aaagcagcgg caaatttatg
    10801 cactgtgata agcggctttt tcagagagaa agactctctg gcgatgggtt tatagtttgt
    10861 actgcgcaga tgttaatgtt ttgtttgctg ttatcttatt gatatttatc ggtctatttt
    10921 ttgttctcag ggcagatatc attaggttta tagattaatc tgatctaccc atttgtgggt
    10981 aaaaatacac ataatgcggg tgacataata gttaattaac ttttgttagc gttttgaaat
    11041 taaaaacacc gttcaccgga agagatatta atttttagcg atgatggagg gataattata
    11101 tttgatctgg cacaagtttt actgatgaag gatgtaactt gtgccagggg tattttgcat
    11161 tacggtaata attattactt acagataacc agcgatccgt tattggcaat attgtttcag
    11221 tagtgagtag tgttctgcct gaatacggta acggtaaacc ggacgccccg tgacgccata
    11281 atggatactg gtgaacaaga tgtggcagtt gaccagccag atgaggtatt tacggcagga
    11341 aacacgcgag atgttaacct cgttagccag ctcgtcggtt gaaaactcat agtcctgatg
    11401 cgcgtcaatc cactggcaca gcgtgcgtaa cgtctgcggc gttaagcctt ttggcaagcg
    11461 acgaggatcc tgctcgttgg agctgctgcc gtggattaac tgatcaagct cggcctggtc
    11521 ataatactga tgtttttcca gcgccatttt cttttgccgc cagccggtga gcgcctcttc
    11581 aaagcgggaa gcctggaagg gtttgatcag gtaatccacg acaccgtaat gcagcgaatc
    11641 tttaatggtt gccgcatcgg ctgcggagga gatgacaatc acatcacttt tgcaacgcgc
    11701 gttatgcagg acaggcagta aatcgagccc gttctctttt tgcatataga tatcgagcaa
    11761 tatcaggtcg ataggcgtat cgctattgaa gataatctct ttggctttct ccagcgtcga
    11821 ggctgttcca cagcattgaa agcctgggat ttgtgctacg tatcggcgat tcagctccgc
    11881 gaccattgcg tcgtcatcga taattaatac attgatcatc tgttcgacct ctccccgtcc
    11941 cagggtatct ggacaaaaaa ttgtgtgaaa atcccgggtt ccgattccac ggcgatgctg
    12001 ccgccgagat tttctacctg ttgtttgaca agtgctaaac cgacgcctcg ctcgcttcct
    12061 tttgtcgaga cacctttgtc aaaaatgtga tcgattttgt cgggtgcgat ccccggtcca
    12121 tcatcgttaa cttcgcagtg cagccagccg tgacggtagt gcaatgttac gctaatttcg
    12181 ccaccgggtt ccggccctaa tgcttccagc gcgttttcta tcagatttcc caatgtggta
    12241 atcagcgtcg cgacctggtc ctcactgccg ctgtctggca gctggctttc actgtttaaa
    12301 atcagcgtat ggcctaaatc ggtcgcgcgg ttaatcttgc tgattaaaaa accagcgata
    12361 accggagatt tgatcttacc cagcagagag ccaatctctt cctgatagtt attggctgtt
    12421 ttgagaatgt aatcttccaa ctgcttataa ctcttcagat gcaataatcc gagaatcaca
    12481 tgcaatttat tcataaattc gtgggatcgt tcacgaagtg catcagcata gttgaccaga
    12541 ccgtcgagtc gctgcatcag tttacgaact tcagttttgt ccctgaaggt tgaaatggca
    12601 ccgatgataa cgccattact gcgcaccgga acggtgttga tcagtaatag ccgatcttta
    12661 atcgtaatct cttcgtcgcg gcgcggcgta ccgtcgcgta acacttccga gacatctacc
    12721 acctgtgacc atgagtggct gagcgtcgac agtttctcat cgtcctgcga cttacggtaa
    12781 ttcagcaatt cttgtgcggc atcgttgatc agcgtgacct cgccgcgatc gtccacggca
    12841 acgacgcctt ctttgataga ctgcaacatg gcctggcgtt gctcaaacag cgtggagatt
    12901 tcgtagggtt ccaggccgaa aaggattttt ttcagtactt taaccagaat gcaggtgcca
    12961 atcagtccga ccagcatgcc aaataatact gaccagataa tgctccagcg actgtcattg
    13021 atctgttggg tcacgcggct taactcaagg ccgatcgcca ccacgccaat ttgtttatga
    13081 ttttcatcgt agatgggggt aaatacgcgt aaagcctgcg ccagaaaacc gcgattgata
    13141 gcgacatttt cttcgccatt cagcgcttta aggatgtcat cacctttaaa tggctgacca
    13201 atacgctggg cttcaggatg cgagtagcga agactttgca tatcggtaac gacaataaac
    13261 agcagatcgt tgcgtttgcg cacggcttcc gcgatggcct ggattccgct ctcctgcggt
    13321 tttttctgca agccctgacg gatttccggc gagtcggcga gagtacgcgc cactgccagt
    13381 gccttgttgg ctagcccgtc tcgcgtcata tcactgattt gcgagaagta aatcagatgc
    13441 accaccaata gcaccgagaa cagtaccgcg ctgaccatta agatcactgt ggtactcaat
    13501 ttcatcggac gtttgcgtaa catgcggtag ggcaatgaat gtctcatcag cttccttgtg
    13561 tgacaaattt cttaagcatt atctctgatg aggcgggtaa ttcaaaggga gtaagaatga
    13621 ttggctatat aggggaagag actctggcaa cggaaactgc cagtgctgta tgaggattcc
    13681 agggctatgc ttatagtgaa aaccatactg atgagagagg gaaggtcatg gatcaggcgc
    13741 tactggacgg gggttatcgc tgttataccg gcgaaaagat cgatgtctat ttcaacactg
    13801 cgatatgtca gcattctggc aattgcgtac gtggcaacgg caagttattt aatctcaaac
    13861 gaaagccgtg gatcatgccg gatgaagtcg acgttgccac tgtggttaaa gtgattgata
    13921 cgtgcccgag cggcgcgctg aaataccgtc ataaataagc gagggtaaaa tggaaatacg
    13981 cgaaggccac aataaatttt acattaatga tgaacagggc aagcaaatcg ctgaaattgt
    14041 ctttgtgccg accggagaga atttagcgat tatcgaacat accgatgtcg atgaaagcct
    14101 gaaagggcaa gggattggta aacagctggt tgcgaaagtc gtggaaaaaa tgcgtcggga
    14161 aaaacgaaaa attatcccat tatgtccatt tgcgaaacac gaatttgata aaacgcggga
    14221 gtatgatgat attcgcagtt gatggaagag tacagattca cgatattttt cgtgctctcc
    14281 gcggtgtgat gcaggagagc atctgaaggg tagggggatg cacaaagaat gggcagagag
    14341 cgcgtttttt tgtcccaagt catccccttt actgagcaaa aaaaagaata tctcctatat
    14401 gagaatcatc aatcggggtt aataagtttt gcgtccccag agcgttcaat attgatagga
    14461 gtcatattat ggaaggtaaa aacaagttca atacttatgt tgtttctttt gattatccat
    14521 catcttattc ctcagtgttc ttaaggttaa gatcattgat gtatgatatg aatttctcct
    14581 ctatcgtggc tgatgaatat gggataccac gacaattgaa tgaaaactcc ttcgcaataa
    14641 cgacatcgtt agccgcaagt gaaatcgaag atttaatcag gcttaaatgc ttagacttac
    14701 cggatattga ctttgacctg aacattatga cagttgatga ctatttccgt cagttttaca
    14761 agtagccagg aaggataaga agaacataaa aaatggcact attctctaaa atattaattt
    14821 tttatgtgat tggtgtgaac atatccttta tcattatctg gtttatctca catgagaaaa
    14881 cacatattcg tttacttagt gcattcctgg tcggaataac ctggccaatg agtctgcctg
    14941 tggcattact tttttctctc ttttaggagc tgtagttgag tggacccgct ggttatcgtg
    15001 aaacgaagcg tctctcctca aaactggatt tgcggaacga tgaacgaagc agtcgcctga
    15061 ctgcttcgtt cattaaagtg aaatttattt ctgtgggcgc atcgccggga agagaataac
    15121 gtcacgaata gtatgactgt ttgtaaacag cataatcatt cggtcgatac caatacccag
    15181 accagcggtt ggcggcagac catattccag cgcagtcacg taatcttcgt catagaacat
    15241 ggcttcgtcg tcacctgcag ctttagcgtt aacctgttcc tggaaacgtt cagcctgatc
    15301 ttctgcgtcg tttaattcgc taaaaccatt accgatttca cgaccaccga tgaagaattc
    15361 aaaacggtcg gtgatttccg ggttaacatc attacggcgt gccagcggtg acacttctgc
    15421 cggatattcc gtaataaagg ttggctgaat cagatgtgct tctgccactt catcaaagat
    15481 ctccgtgaca atacgtccca gcccccagct tttctctacc gtaattccga tagattcagc
    15541 taatgcttta gcagcatcaa aattatccag gtcggccata tcggtttctg gacgatattt
    15601 tttgattgct tcgcgcatgg tgagtttttc aaacggtttg ccgaaatcaa acacatgctc
    15661 gccataagtg actttagtgg tacccagaac ctcttgtgcc agggtgcgga acagggactc
    15721 tgtcagttca atcaaatcgt ggtaatccgc atacgccatg tagagttcca tcattgtgaa
    15781 ctcaggatta tggcgaacag aaataccttc attacggaag ttacggttga tttcgaatac
    15841 ccgttcaaaa ccgcctacaa ccagacgttt cagatacagc tccggcgcga tacgcagata
    15901 catatctaaa tccagagcat tatgatgggt aataaatggg cgagcagatg ccccacctgg
    15961 aattacctgc atcatcgggg tttctacttc cataaagccg cgcgcgacca tgaactgacg
    16021 gatagcggcc agaatttttg aacggacaac aaacgtttga cgggatttat cgttagcgat
    16081 gaggtccaga taacgttgac gataacgaac ttcctgatcc tgcagaccat ggaatttatc
    16141 tggtaaagga cgtagtgctt tagtcagcag gcgcagctca gtacagtgaa tggaaagctc
    16201 acccgtttgc gtcttaaaca gcgtaccgcg ggcaccgata atgtcaccca gatcccattt
    16261 tttaaactga tcgttataaa caccttctgg caggctatct cgtgcgacgt acagttgaat
    16321 acggccaccg acatcctgca acgttacaaa ggaggctttc cccatgatac gacgggtcat
    16381 cattcggcca gcaaccgaga cttcaatgtt taaggattcc agttcctggt tatccttcgc
    16441 atcaaactct tcgtgcaact ggtcagaggt atggtcgcgg cgaaaatcat tgggaaacgc
    16501 cacaccttgc tgacgtagtg ccgccagttt ttcgcggcga tttctcagtt catcgttaaa
    16561 atcaatagcc tcattggctc cccgtgtttc ttgttcagac attttggttc ctctaaatcc
    16621 agctttcaat ttagcgtaga taaagagaca gatcggtaca ctgtattatc tgcctcgact
    16681 attaataact cagtttatct tatcgaaact aacgcttacc tcaagttgat atcactaata
    16741 aataaccatc aaaatggttt aaatgactta ttaatctcga tttactagtg tgataaactg
    16801 aaagttaatt tattcataac caattgtttt tacgacagtt aatcgttgct ctcctgtacc
    16861 atattcgtcg gcgtgctaaa caggaaacgg gtagcaaagg ccaatacgac gatgatcgcg
    16921 acacaagcca acgtccactc tcccatctga gaaaagaatc gctggtaagc tgcaattgcc
    16981 ataccgctaa tttgcgactc tgtcgtctgc tgtgccacga cgcctgccag ccagttggcg
    17041 accgcgcctg ttgccagcat ataaatcccg gttaatacgc cagacatttt cagacgcgtg
    17101 atttgcgcaa tcgccaccgg gtcaataaag agttcggcaa agcccattag cgccaacccg
    17161 gatatcatca cgcccattga cgcttgaccg tcagccgctg catgtcgggc atcaaatgcc
    17221 aacaacataa agccacaagc catcagcagt aagccaaagg caaacttcag ccagacgcgc
    17281 aatgttgagt tgccgcggct ttctggcgac gccagccagg ccagtacaac cccagcgagc
    17341 atcaccgcaa ttgcattcac cgactggaat agtgctgtag gtacttcaat attgaatgcc
    17401 tgacgattca caaagcgatc gataaacaag ctgatggtac tgccgccctg ttgtgccagt
    17461 acccagaaca atgtcccgac aaacatcaac aatacaattt gccaaagagc acggcggtgt
    17521 tcggggaatt tgatcatcat gcgggcaatg atttgtgcgg caatgaggca aacgatcgcc
    17581 agcaaatatc cggaccagtc gttctccagc agcagggtaa aaaatactgg ggctaaacag
    17641 agcatcacca ctaaccagct ccataccggt aaggcaaatt tgacgctagt gagcgctttt
    17701 ttatccatac tacgcgtgga ctggaaatga cgatgcccgc ttaagaaaat caacaaaccg
    17761 ataaacatgc cgccacccgc aagggcaaag ccaacatgcc atccatacca ctgagcagcc
    17821 aggccgcagg cgattggggc ggcgatagaa ccgatattgc ccgcagcata cagcagcgaa
    17881 aaaccgccat cacgtcgatg atcgttctcg tcgtagagct cgccaagcaa acagctgatg
    17941 tttgatttga ataaaccgta gccacaaata atgattgcca gcgccagata caggctaaag
    18001 gttgaatttg tatcaatacc cagcaccaca tggccaagag tcattaacag cgcgccggca
    18061 atcactgcag tgcggttgcc gagcaggcgg tcggcaagcc agccgccgag aataggggta
    18121 acgtaaacca gagaagcata tgcgctgaac aggctgatgg catggttatc atcaaaacca
    18181 agctgatggg tgagatagag aatgagcaag gcacgcatgc cgtaaaaact gaagtactcc
    18241 cagatttgga tcgccacgat atagtatatc gcgcgcggct gtgagggtgt tttcatgtgt
    18301 tctccttatg agcaaaaaag ggaagtggca agccacttcc cttgtacgag ctaattattt
    18361 tttgctttct tctttcaata ccttaacggt atagcggcca tcagcctgac ggtatgcacc
    18421 gtgaatatcg gtttcaaagc ccggatagtg agcgccgatt tcacacagca tctgcaggaa
    18481 ctccagaacc ggacggcttt cttcggtgat catttcaccc ggcattacca gaggaactcc
    18541 cggcggatac ggaaggatca tattggcgtt aatacgacct accatttcgt cgaggtaaac
    18601 ttcttcggtc ataccgtgca gctctttctg gaatgcagca tacggagtca ttaccatcgt
    18661 cggcagcact tcaaatgcgc gatacatcag atccggcaga ttgtggtgaa caatcagttt
    18721 gtggatattc tgagccagtt cctgaatacg catgttttca tagaattcag gatcttcacg
    18781 atacagagac ggcagcatgt ttttcacacg caggttcagg tcgaacgcac gtttgaagtc
    18841 agtcagagca cgcagcaggc tcagtgcttt ggtcttatcg ataccgatgc tgaacaggaa
    18901 cagcaggtta tacggaccgg ttttctcaac aacgatgcca tgttcgtcga ggtatttcgc
    18961 cacgatgctg gccggaatac caaagtcgct catggtgccg tctttttcca tccccggagt
    19021 cagcagggtg actttgatcg ggtcaagata catgtgctcg ttatcgatgt ttttgaagcc
    19081 gtgccaggtg ctgtcagaac gcagcggcca gcattcagtc gtatcgatat gatccggttg
    19141 ccatacatca aagaaccagc catcagattc cgttctcaga cgtttgatct ctttacggaa
    19201 tttgatcgca cgttcaatgg aaccgttgat cagacgctta cctgcattgc ctttcatcat
    19261 cgccgcagcg gtttcagtgg acgccacgat accgtagtgc ggagaagtgg tggtgtgcat
    19321 catgtaggct tcgttaaagg tttcttcgtt tacgtcacct tttacgtgga tcatggaagc
    19381 ctgagagaac gccgccagca gtttgtgagt ggactgggtt tcgtaaatca ctttcccttc
    19441 tacacggcca ccgctcatac cgcatttacc ttcgtaaatc ggtgagaagt tggtgtaagg
    19501 cacccacgcg gagtcaaagt ggatggattt cacatccagt gttttcttga tgaagtcggt
    19561 gttgtacagc agaccatcat aggtagagtt agtaattaca gcatgtaccg gccaggttgc
    19621 gtttggtgtt tccttcacgc gcttagcaat ggtagcgtgc tggaattcac tctgtgggat
    19681 accaccaaga ataccgtaag cgttacgggt cgggcggaaa tagattggcg taacatcgct
    19741 catcatcatc aggtgggtca gcgatttgtg gcagttacgg tcaatcagaa tggtgctgcc
    19801 tgccggagca gagtacatac caacaatttt gttcgcagtg gaagtaccgt tggtcaccat
    19861 gtagctgcgg tctgcgttaa agacgcgagc gatatactgt tctgcttctt tgtgtggacc
    19921 actgtgatcc agcagagaac ccagttcaga tactgaaatg gaaatatcag atttcatggt
    19981 attcggacca aagaaatcat agaacaggct acctaccggg cttttctgga atgcagtacc
    20041 gcccatgtga ccaggagtac agaaagtata tttaccttca cgaacatatt taaacagtgc
    20101 tttagtcagc ggaggcagaa tagtgttgat atattcgtca gtggtctgct tgatcttgtt
    20161 agcaatatct tcagcagcac ccagcgcata ttcaaagaag ctaatctgta aacgcaggtc
    20221 attcaggctt acatcgagag tggaatacgt attagcgaac gcgtacaacg gcaggttctc
    20281 gttcattttg ctaatttctt cgcacagctc gagattatat ttatcccagt caaaaataac
    20341 gccgcacaga cgcgcattgt tttcgatcag ttttaataag tcgtcacggt cgttcgggta
    20401 aacaatctgg aagttcagac gttcaagcgc gcgatgaagt tcacggatgg gttcttcttt
    20461 aaaataaacc cccatgtgat tcaatattgc aataacgttc atagtcatat ctccaggtaa
    20521 aaaaggcccc tcccaacaca tgggacaaaa tgaaaggagg agcctcggaa aatactttta
    20581 attaatgtgc gttagacgcg gtgtggttat ccattgagtg gctctggcgc tcgtgcattt
    20641 tgcgagcgta gaacatcagg ataatcaggc tgacgatgaa ggtacctgcc agctcgaagg
    20701 agcttgcgcc catcagcgcg atgaagcaga acacgcaacc cagtacagag cagatcaggc
    20761 tgacaaagtt gcggatgtta acgccttcaa aacgaatcag gtcaacgcaa gagtagaagt
    20821 acggcagcat agtcagcagt actgcgatac cggtcagttc accgaacagg tcagatgctt
    20881 taccaccggc agagttcatc agagtgatga ggatcatcag ggcagtcatt ttcactgcag
    20941 ccagcagcag accttttttc ggaataccgt tgctgtcgac ttcaccataa actttcggga
    21001 agttaccgtc gttagcggca cgtacacctg cctggcctac caacatcatc caggagccca
    21061 gagaagtcag gcacgcaaag gcggtgaatg cagaaaccag cggcgcagcc cagttaccga
    21121 ggatagttga agcactgatt gcaaacggag caccggaagc cgccattaca gaagacggat
    21181 acataccgga aagcacctga gtcgcagcga tgtaaacaat acctgctaaa ccagtaccca
    21241 gcatggttgc cagcggaacg gtacgtttcg ggtttttaac cataccagta cttacagctg
    21301 cggattcaac acccacgaag gcccacaggc agagcagaat acttttaatg atcgcatgac
    21361 catcagtggt atccgcagta ttccagttag ctgcataagt tgccgcatca aaccaatgcc
    21421 agccaacaat agcagtcatc accacaggaa taagaaccag caccagacca atagtggtta
    21481 aacggcttac ccaggtaccg ccgagcatat ttacaaaggt aaatacccag acgatagcaa
    21541 tacaggcgat acccgccgga acaggatcat ttaatactgg gaagaaggtg gaaagataag
    21601 atacagcggt aataccaatc gccaggttac caatccagtt agcatggtaa taaagaacac
    21661 ctgtctgaaa accaaatgca ggagaaattt ctccggcata agcaattggg ccaccttgtt
    21721 gcgggttttt tgttgccagt cgggcatata catacgccag cgacattgca ccaataatag
    21781 agataatcca accccagata gcaataccac cgatacttgc taggttcgca ggtaataatg
    21841 caataccgct ccccatcata ttaccggcaa caacaccggt acaggcaaat agcccgatct
    21901 tcttggcaga actcatgctc ttctcctaat ttcatttttg aatttggagt ccgggtcatg
    21961 atgtataact atttcctgac cagaccaaac tggcgctaag attactcacg aaaaaaggat
    22021 taatcctaaa gattaggtga aataacacaa aagtttctgt aagtgagaac ttgaggtttt
    22081 ttattaacac atcaggatcg caagttgata tcatgaaaag ataaacattc aatgtttaca
    22141 atagattgcg tgacgtcttc tggttaaatt tatgtaataa aaattatgcg gcaaataaat
    22201 tgccgcaaca tattatacca acaggaacat acaaaaactc aacaacaaat attcccgagc
    22261 atgaatcaac ccgggggtta cttattctga agcgagaaat ttgtcgagat aaggtacaac
    22321 ataaggaaca gaagtctgga atataccatt ttcaatccag taaagggtgt ttgcccctgg
    22381 gcgtaaatta aaggcggtga gatatgcatc agccgcttcc cggttcatcc ccttcatttc
    22441 ataaaccttg ccaagcaata cataatttag ccaggacatt tcaagatcaa taccagtatt
    22501 tatcgcctgg taagactcat ctgttttacc ttttaccaga gcactgaccg cttttatttg
    22561 atatataatg gacaggttat tcaattccgg tagtgtaaca atgttatcta tttctgtgtt
    22621 cagtgctgct aattgttttt catctaaagg atgttgagaa tggcgcacga tatcaactaa
    22681 tgctttttct gctctcgcgt aggtaaattc tggggatgat tgaacaatct cacctaataa
    22741 ttcactggca cggttcaatg atttatcatc gccatgcagt aaataatcat gtgcctgata
    22801 aaaattagtt aataacgcac cacgatgagg caaaatttgc tggagcgtct cctgcattcg
    22861 ttgtggccac ggttggttta acgcttttga taaactctcc agtaaatcat tttgaatcgc
    22921 cagctgatta ccgttagtga tgacataacg tttatccagc atggttgaac catctgcatt
    22981 gtctaccaat tttatcgaca taaagcattg ttgagcacgg tattggcgct gattaacaaa
    23041 cgcaatagat aatgttttac cggaactgct cggttcatca atgttgtagt tgattttgtc
    23101 atgcaccata aaggtggaga aggtgttaag tgatgtcgcc accaaatcac ccacgcctat
    23161 cgcgtaagag agctgatacg gggaactcca gctgttacaa cttttattta ccatattaat
    23221 gtcaatatcg cgtggattga gcaaaatacg cgatttgctc atcggaagac gtgtatcaag
    23281 acttgaaaac gctaccagtg ctacacagat acctaaggac aacaggaaaa aaaaccatac
    23341 ccaaaaggta gtgaatcgtt tgcttttaac tggggattgt tcaggtggcg ttgcggtgtt
    23401 ttgaatgtta agactgtggg agggagaatc tgtggcagga accgcctctg gtataggggg
    23461 aggcgaagat agcattattt cctctccctc ttcttcgctg taccagataa ccggcaccat
    23521 taatttatag ccgcgctttg gtacagtagc gatatagaca ggactatctt catcattatc
    23581 ttttaatgac ttacgtagtt ctgagatact ctgcgtcaca acgtgattgg tgacaatact
    23641 tctcttccag acattatcga taagttcatc cctgctaagt acttcgccac tgtgttgagc
    23701 aaagaaaacc agaaggtcga ttaatctcgg ctcaagggta agttgacgcc cattgcggct
    23761 aatttggttt atggacggag taacaagcca ttcgccaacg cgaactacag gttgttgcat
    23821 aatagaaact cattcgaaaa gggaatgatg caatgataat tgccacaacc tatttttacc
    23881 atctatagat gggtttattt acatattatt ggtgaatgca agacgttatt tttaccagcc
    23941 ataaacttct tgattacatg gtattacgaa aggattttac tgagaaccag aagtaatatt
    24001 ccttaccatc aaaattcatc atctttgcca aagaaaaatg ttcagaaaat aatccatgga
    24061 aaattgtccg gagcgcttac tattttaatg gattgttagt ctttgcatga gcaagcggac
    24121 tgatacattt ctctttgttc tcattcagaa aatctcatca gtcgccgttc agaccggtgt
    24181 tgtgctttat ggatgcgtta cgctcctgat gacgtcattt gacgttcaac agcatcacgg
    24241 ggtcgcacga catttcacgt cagttagtgc tatcgctcag gaacaaattt tcccgaattg
    24301 ggatatgccc gcaaattgct ggtgatgtgg ggagaatctg gttgagttcg gtagaattga
    24361 tttggcggca gaacgcttaa atcgtggcgt cctgaaacga aaaacggacc tccgtggagg
    24421 tccgtttata tgaatttggt gcccggactc ggaatgaaac tggttatata accaattgtt
    24481 ttgtattgat tgttttgtct ttgttgtttt cgaggccacc aatgaggccc ccatgtgcaa
    24541 ttcgaattct tttgacgcta taatccctct tttttgtgct ttgctgtaat cattttgtct
    24601 ggttcgatat gctgaattgc ggaatcgaag taacaacaaa atacagagat ttataaaata
    24661 cctggctacc tgtgttaggg agcgttaacg cacagatttt gtttatggct gccagaaatg
    24721 atctgatggt ttgtcgtctg gctctgcaag taccctgttg atatcccggg aaccatgaac
    24781 gatacgttcg atacgaactt cagtatccag taccctgaac agcagaaggt aacgaccata
    24841 cgaacaacct ctgatcccag cccctaattc gggtctttct ctgtagagta ctggcgattc
    24901 ggctatcaac agacattgtt ggtaaagctc ttcggtaaaa ctgattgcac ggacaggatt
    24961 atcctgagcg atccagtcac ctatagcttc aagatcgtgt tctgctaatg gtgaaacagt
    25021 cagtttcata ttggctgttc accttctgcc atctttttgt atttgtgcgt cagtcgcccg
    25081 aatacctctt cagcactttt actttcaccg ctgttgatgc ctgcggtaac ggcgctctgg
    25141 agtgattcaa gctttatctg ctgttcccgt tcttccagag cacgaagtcc ggcacgaatg
    25201 acttcacttg tattgttata ccgaccactt tctatttgtt cgcggataaa cgtttcaaag
    25261 taaggactga gggatacact ggtaggcatg gtgattttct cgccgattga ttgctatcaa
    25321 ttaataatag ttaatattcg cgaattgtaa aatacctctt ttatgccttg cggcttttgt
    25381 tcactgtgct tgccagggct tccgggtagt gtctggcaat aatatcatta agcctgtcaa
    25441 ccagttctgg tttcctgaac gtgatgtgta ctgagccttt cttaaagtat ttaatactga
    25501 acatctcgtc ttcatagttc tctttaccct gaacagactg gatatgatca tccagacgaa
    25561 gggtgatgtt ttcccggttg tcggggatcg gtttgccgct gaacaggtgt agcattcttt
    25621 ccagatcggc aagtcgatct gcctgttgcc cggtgataaa atgaaacccc caccggtccc
    25681 acctcaccag gttgttgacg ataattttac tgccaaattt acagggtgag ttggttttgt
    25741 aattccagct cagtcccctg aagaggttga tcacccctcg ctcaaacacc tcatccttat
    25801 tctggtgcag ttgttcaaat gtgctcagaa tgtttgccgc actgatttcc ggaaaattat
    25861 cgtattccag tgaccggtac catgtatcac gtgcctgtgc atccattaat gacagcatcc
    25921 cggaacgttg catcaggtca cgccagattt cgcggtcaag attccgggta atggctttca
    25981 ttgctgtttc gggtttctcc atcagccagc aaccgcagcg aaaattctgt tttatggccc
    26041 agtccggggc ggttttacct ccaatacttt ctgtcattgc tgagatttct gtcagttgcc
    26101 ggataagtgt gtcgatttgc atcagtgtgg tctttcgtac agccagaatg tgtccgattg
    26161 aggtggaaca gataacgtcc gtatgcccgt tgaccacttc aggacgggag attgtgatac
    26221 ctgacataat cgatattcct ttttgcagat aaatgaaaac gccagccctg ttcaggctgg
    26281 cgtcagtgga atgaagtctg gtgagtgata tttaccggtt ctggtgcaga agctgcagat
    26341 gacgaagagt acggacatcc tcccgggcct gttcgataca gggcaacagt gctgccgaat
    26401 ccgttttatc ctcatcgttg tcaaagataa ttcccgattc gcagtcgata ttgtcctgca
    26461 gccacgcaat gaggatatcc agcgctgttt cagtggttaa tgatttcatg ttgtttctgg
    26521 ttaccagtcg aaagtgggta aacccggcag gcattcagcg ctggcatcca gatgaatgag
    26581 actgacgcca taacgccgga taagtgtgac aaccagacgg cggaacattt ttgacagccc
    26641 caggcgttta agccgcagaa ccgggtatga ccacgcatcg gtacgtaaca gatatccgct
    26701 acctgtaaaa tgaatccatt ctgattcgcc gaagtcactg gtctggtgtg acagcgagta
    26761 caaccaggcg ttgtctgttt ccgtgatatg tgcggtgctg cagcgtatgc cggtaagggt
    26821 tgttaacggt ggtggtgtgc aggttgactg ttggtcggag tcgtccacca cacggagtga
    26881 ataaccgttt tcagcgactt tgttaatcag ttcagcgagc tcaatgccat cgacgtcaac
    26941 gacaatgcgc cccatgttca gtgcctgcac gttaatgctg tcggcttcca gtgtcagggc
    27001 cagtttcatt gttgcgcctc cggatgctta ccctgggtaa tgttatttac cattctgtaa
    27061 ttgtcgcggg tcatcaggcc tgttgcccgg cgagcccgga ggatatcgat gctgtttatt
    27121 aactgagagc gggtacaggc gctgaatcct ggctggtcag ttcgtaccag cgcgtatttt
    27181 tccacgagaa agttcaccgc atcacacagt gaaatgcctg cctcaatatg ctgctcaatc
    27241 acacgttcat tggcgaacgg tgtgtcattc agcgtgaggc cgtagtgctg gtccagcagt
    27301 cgggtgagca gtgtctgcca gatttcaacc ggagacgggc agcgcgatgc cgcccgtacg
    27361 tgtgtgtcgg gtaatgtttt cataatgaag attttcctga tatgagcgat taaattttga
    27421 ggagaatggt gctgtggtgc accgggcagt agcagaggtt tattctggtg gtttatgttt
    27481 tcgttccggg tgtcggataa atggcgatat acacgtaacc acaactgccc agggtatcgg
    27541 catcgcaggt cagccctttt gcatacagcg tgacggtatg ctgatggcgg ggattcagtt
    27601 caccgctggt gagcatgagt tccagttgtt tcatcagcag cggaaaggct tggtccagat
    27661 ggtatgcatc cgcatcgctg aaccggcctc tgatgtcggc gcggtctgca aggtaatgca
    27721 accggttacc ctcctgcacc agacgtgccc cgaaacaggg cgtcactgtg caggggagtc
    27781 cccaccaggg gcggtcgtga ttgtcgtcag gatgcgttgt cccggagagt gtgtctgaca
    27841 cgataaaatc ctcacagaaa atcggtgaat gatagtttaa cgatgacggg tcagccggcg
    27901 cgcagaccgg cttcggtaaa ggtgtattcg ttgatgagaa gggcctcatc cacagtggca
    27961 tctgaggtca gccagtcgta ctcgttttcc agttgacggt acagccagaa ggccaggtcg
    28021 cgcagggctt cgctgacagc ggcttccgca tcggttgtca ttatctgacc agtcggactg
    28081 ttccgggtga cggtgatatc catgctgtac ggatggcagt agcgtccccg gtggtggatt
    28141 tcagcctgca gttgccagaa gttacgcttc tgcaccgcct gcagggcatc cgcgatacga
    28201 tgaagctcac ggtcctgcgg ggcatactcc cggattatcc tggctgttgc tgactgccag
    28261 tgccagcgtc cttcaaaaca ggctccgtcc ccctggctgc agaatccgct gaaccagatg
    28321 cacggctctt ccataaacct gccgttgctg agctggaaaa cgcgctggcg gaggtcaatc
    28381 ccgaaaatgt cacagacctt cctgaagtct tcatacacgt tttcatacca gttgctgtca
    28441 gcatgatgct gacggtaccc gtcgcgggct ttttcccgtg cggtggcgga tagctcattc
    28501 agtgtataca cggtggaggt gataattctg ctcatctgaa tgcccgtcct gattcagttc
    28561 agggtgacgc tcatcagccg gacatacggg ccaaaactgt ccttacggcg ttcagcaaac
    28621 acggccagca caccgggaat atcctgcact tcacgaccgg tatacgcctc agcactgccg
    28681 tgccagcggt atttgccggt acagaaagga aaaagacggg atgccggatg ttgttggtga
    28741 atacgcatgg cttcaccacg ggtgatgatt ttcatagtgt gatacctctg aagacagaag
    28801 ataaaagtga aaacaagcgt gatgtggttg tgacgggtta aagcagacca tgttcggcaa
    28861 aggagaaaac ctggttgcca ccgactatca gatggtccgg cacccggata tccaccaggc
    28921 ccagtgcctg taccagacgt tccgtgataa ggcggtctgc cttgctgggt gtgacttcac
    28981 cggacgggtg attgtgcgcc agcaccacgg cagcggcatt gtggtacagg gcgcgtttaa
    29041 tcacttcccg gggatggact tccgtgcggt tgatggtgcc ggtgaagagg gtttcaccgg
    29101 caatcagttg attctggtta ttcagataca gcacccggaa ttcttcacgc tccagtcccg
    29161 ccatgttcag aatcagccat tcccgtgccg cacgggtgga ggtgaaggcc acgccgggtt
    29221 catgaagatg gcggtccagg gttttcaggg cccgctgaat gaggctgcgc tcgccgggcg
    29281 tcatctctcc gggcagaaag gaaagctgct gcattgtccc tgcctccatt cagtcgatga
    29341 tgcgcataat ggcgctgcat tccggatgct gcagggcata atcccgcagc cggtaataat
    29401 gaaccgtcat ggcataattc tctgtacgac aggcgtgatg gctgtacgcc atcagacagg
    29461 cggcaatgcc ggcggcttcc gggctcattt cagcgcggtt accgttcatg acgttaaaca
    29521 gcacccatgt ttcgtcatca tcgtcatccg gttcgggtgc cataaatgcg ccaccgttgt
    29581 tcagggtgta cagattccag ataccaccgc agtagtcttc atacagacgg tccatccagc
    29641 cgaagacccg ggactccagg gtcacccact gtggaatgag gccaaaatgc tgcggccaga
    29701 agctgatgcg ctgttcatca gggacgaggc tggcaaccaa ctgaggctgg tcattccctg
    29761 atgcagcggt tacggaaaca gaagatgtgg tggaattatg tgaaatggtt ttcatgaaat
    29821 gactccttaa aggacaaata aatgcgtggt tttcagggta atggctgaat cagcgcaggc
    29881 tctccagcag cgtttccgcc atcacccaca atgcgcggtt gagcttaatg tcagtgtcaa
    29941 tgctgtgaat ggcacgggta tggatacgtt ttcctttagc gctgcgacct gaaattccgc
    30001 ctttcagcat attctcctga atggtctgat aagcactcca caggtcctta ccgtaatcct
    30061 cccggcgtcg tggcgtcaga atgtcggcgg tggtgacggg ctgatgttcg tcaccataac
    30121 ggtaagtcag tgccgcctgt gccagcgcct ggcgtgccgg tggcggcaga atcagcgact
    30181 gcatggcatc acgcttttcc tcaatccggt caaaaacccc caccacctcg taagcccctt
    30241 caatgactct gtccaccaca tttccccggt gcggaacacg cacttccccc agagactgac
    30301 cacagacgca tccgttctgg cagacgaacc tgaagtaacc cggcagcatc tggtagctgg
    30361 aggtaccgtc atgagagttg agcagaataa tttcagggac atgttctccg tttatctctc
    30421 cggcccgccg cagacgcagc atgtgttttg tgtatccccg gcggcccggg tcgcgcacac
    30481 gggtctggca ggcgaagaat ggctgaaagc cttcccgctg caggctttcc agtacggtga
    30541 tggtggggat gtacgtatag ttctggcttc gggacgtgtg tttatcttcc ccgaaaatcc
    30601 ccggcacata gtgcatcagt tcttcgtgtg tcagcggatg gtcacggcgt atctggttcg
    30661 cataaccaaa acgactggct agtcgcataa tttgctcctt atcggtggtt aagatttact
    30721 ggtgtaataa atgaaaaagc cacgtctccc ggagaagacg cggcctgatg ggtgaaatga
    30781 atgacgttta ttgtctgaga agaccgtcac tggcgagccg cgtattaagc acctttccgg
    30841 catcgggttc agaattccgg cagcgccagc gtcgctgacc ttccgcgttg cggataaccc
    30901 gacggaaatg cattcccggg tcatcctgga taaacacatt gcggtaagcg gttatgacag
    30961 caaccgcctg cccgtatgag aaagatcctt cagccaggac atactctgtg taactcggca
    31021 tatctgtttt ctcctgataa ataaaaagcc tctgccacgc gttgtgacag aggctgaagc
    31081 atgaaactga cttcagggat cagttaacgt tttttccgga aacggtaatc agcagtggat
    31141 ggtagtcctg gggattgaaa accgataacg gcagactgac acgatggccg ttactttctt
    31201 cagttgcttt aatgatttcg gttgtggcga cattttccac gcactccgtt tccagaaatg
    31261 cgtctgtggt tcgcgtggca ttactgtcac caaaagcttc cgtttccatt tttctggtca
    31321 ccagcgtctg accatatttg tctttgagtt gcagagtgat ggtgaggggg ccaaatcctt
    31381 catcgtttcc gccattatcc agccggaact ggtaagcaca aatatttccc gggagccata
    31441 tcgtatctgt attgcgtata ctgatgtaac gttgatcctg tgcccggagt ggggcagacc
    31501 acgttaaccc cagaatgaag gcggtaatca tgcaggtttt gaacaggtga atcatggtat
    31561 ttacctctct gagtcatgac gatgacactg acaaatcagg tgataaa
//
LOCUS       DAZFKF010000054        30479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFKF010000054 DAZFKF010000000
VERSION     DAZFKF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.054"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            135..1718
                     /gene="yegH"
                     /locus_tag="ACHKA1_002916"
     CDS             135..1718
                     /gene="yegH"
                     /locus_tag="ACHKA1_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO5680537.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2170..4023)
                     /gene="asmA"
                     /locus_tag="ACHKA1_002917"
     CDS             complement(2170..4023)
                     /gene="asmA"
                     /locus_tag="ACHKA1_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO5680538.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     TAHVEMADTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQILRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4045..4626)
                     /gene="dcd"
                     /locus_tag="ACHKA1_002918"
     CDS             complement(4045..4626)
                     /gene="dcd"
                     /locus_tag="ACHKA1_002918"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO5680539.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4718..5359)
                     /gene="udk"
                     /locus_tag="ACHKA1_002919"
     CDS             complement(4718..5359)
                     /gene="udk"
                     /locus_tag="ACHKA1_002919"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO5680540.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5677..8994
                     /gene="dgcE"
                     /locus_tag="ACHKA1_002920"
     CDS             5677..8994
                     /gene="dgcE"
                     /locus_tag="ACHKA1_002920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5680541.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(9103..9951)
                     /gene="alkA"
                     /locus_tag="ACHKA1_002921"
     CDS             complement(9103..9951)
                     /gene="alkA"
                     /locus_tag="ACHKA1_002921"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO5680542.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMSLKRAEALIHLANAALEGSLPMAIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            10085..11437
                     /gene="yegD"
                     /locus_tag="ACHKA1_002922"
     CDS             10085..11437
                     /gene="yegD"
                     /locus_tag="ACHKA1_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5680543.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQRVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALEEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11450..13390)
                     /gene="yegI"
                     /locus_tag="ACHKA1_002923"
     CDS             complement(11450..13390)
                     /gene="yegI"
                     /locus_tag="ACHKA1_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO5680544.1"
                     /translation="MKPTLYTATGECVTTGRELGKGGEGAVYDINEFADSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCTVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPAVQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSAVSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEIQQRREAFNRT
                     KMDYDHLVSQIQQLGGLEGFIAKRTMLEKMKDEMLGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMELLRQAAEKLAQAQADLSRC"
     gene            complement(13387..14148)
                     /gene="pphC"
                     /locus_tag="ACHKA1_002924"
     CDS             complement(13387..14148)
                     /gene="pphC"
                     /locus_tag="ACHKA1_002924"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFO5680545.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAINEAMAYVSQKVQHGEFGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMAGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(14145..14804)
                     /gene="yegL"
                     /locus_tag="ACHKA1_002925"
     CDS             complement(14145..14804)
                     /gene="yegL"
                     /locus_tag="ACHKA1_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO5680546.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15025..15084)
                     /gene="ibsA"
                     /locus_tag="ACHKA1_002926"
     CDS             complement(15025..15084)
                     /gene="ibsA"
                     /locus_tag="ACHKA1_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO5680547.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15357..15413)
                     /locus_tag="ACHKA1_002927"
     CDS             complement(15357..15413)
                     /locus_tag="ACHKA1_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5680548.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(15685..15741)
                     /locus_tag="ACHKA1_002928"
     CDS             complement(15685..15741)
                     /locus_tag="ACHKA1_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5680549.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            16020..17267
                     /gene="mdtA"
                     /locus_tag="ACHKA1_002929"
     CDS             16020..17267
                     /gene="mdtA"
                     /locus_tag="ACHKA1_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO5680550.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNEPQSAAPGATKQAQ
                     HPPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATAPEEKATSREYAKKGARS"
     gene            17267..20389
                     /gene="mdtB"
                     /locus_tag="ACHKA1_002930"
     CDS             17267..20389
                     /gene="mdtB"
                     /locus_tag="ACHKA1_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO5680551.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKIFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMLAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20390..23467
                     /gene="mdtC"
                     /locus_tag="ACHKA1_002931"
     CDS             20390..23467
                     /gene="mdtC"
                     /locus_tag="ACHKA1_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO5680552.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23468..24883
                     /gene="mdtD"
                     /locus_tag="ACHKA1_002932"
     CDS             23468..24883
                     /gene="mdtD"
                     /locus_tag="ACHKA1_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5680553.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPIGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24880..26283
                     /gene="baeS"
                     /locus_tag="ACHKA1_002933"
     CDS             24880..26283
                     /gene="baeS"
                     /locus_tag="ACHKA1_002933"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO5680554.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26280..27002
                     /gene="baeR"
                     /locus_tag="ACHKA1_002934"
     CDS             26280..27002
                     /gene="baeR"
                     /locus_tag="ACHKA1_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO5680555.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27193..27525
                     /gene="yegP"
                     /locus_tag="ACHKA1_002935"
     CDS             27193..27525
                     /gene="yegP"
                     /locus_tag="ACHKA1_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO5680556.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27723..28020
                     /locus_tag="ACHKA1_002936"
                     /pseudo
     CDS             27723..28020
                     /locus_tag="ACHKA1_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
     gene            28022..28318
                     /locus_tag="ACHKA1_002937"
     CDS             28022..28318
                     /locus_tag="ACHKA1_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5680557.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            28421..29782
                     /gene="yegQ"
                     /locus_tag="ACHKA1_002938"
     CDS             28421..29782
                     /gene="yegQ"
                     /locus_tag="ACHKA1_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO5680558.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(30112..30429)
                     /locus_tag="ACHKA1_002939"
     CDS             complement(30112..30429)
                     /locus_tag="ACHKA1_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680559.1"
                     /translation="MKKIVAISLFSIFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CVE"
BASE COUNT         7009 a         8139 c         8060 g         7271 t
ORIGIN      
        1 attcagggat aatgtgaggt tattaccctc aaataatatg agataaatag tgctgcaaca
       61 ttgcattttt gccccgattt atccatgatc gaattgtgac atttgtcata caacgaatag
      121 gttttgtact tactatggaa tggattgccg atccgtctat ctgggccggg ttaatcacgc
      181 tgattgtgat cgaactggtc ctcggcattg ataacctggt ctttattgcc atcctcgccg
      241 aaaaactacc gccgaagcag cgcgaccgcg cacgggttac cgggctgttg ctggcgatgt
      301 taatgcgcct gttactgctg gcgtcaatct cctggctggt caccctgact caaccgctgt
      361 tcagcttccg ctcgtttacc tttagcgccc gcgacttaat catgctgttt ggtggtttct
      421 tcttgttgtt caaagccacg atggagctga acgaacggct ggaagggaaa gacagcaata
      481 accccacgca acgcaaaggt gcgaagttct ggggggtggt gacacaaatt gtggtactgg
      541 acgccatctt ctcactcgac tcggtgatta cagccgtcgg gatggtcgac catttactgg
      601 tcatgatggc cgccgtggtt atcgctatca gcctgatgtt gatggccagc aagccgttaa
      661 cgcaatttgt taacagtcac ccgaccatcg ttattctctg cttaagcttc ctgttgatga
      721 ttggctttag cctggtggca gaaggtttcg gcttcgtcat tccgaaaggc tacctgtacg
      781 ctgccattgg tttctcggtg atgatcgagg cgctcaatca gttggctatc tttaaccggc
      841 gacgttttct ttccgctaac cagacgctgc gccagcggac taccgaagcg gtaatgcgcc
      901 tgcttagcgg gcaaaaagaa gatgcggaac tggacgccga aaccgcgtct atgctggtgg
      961 atcatggtaa ccagcagatc tttaatccgc aggaacggcg gatgattgag cgggtactta
     1021 atcttaacca gcgtaccgtc agcagcatta tgacgtcgcg ccacgatatt gagcatatcg
     1081 atctcaacgc gccggaagac gagatccgcc agttgctgga gcgaaatcag catacgcggc
     1141 tggtggttac cgacggtgat gacgcagaag atttgctcgg tgttgttcac gttatcgacc
     1201 tgctacaaca gtcactgcgc ggcgaaccgc tcaacctgcg ggtgttgatt cgccagccgc
     1261 tggtgttccc ggaaaccttg ccgttgttac ctgctctgga gcagttccgt aatgcccgca
     1321 cgcactttgc ttttgtggtg gatgagtttg gttcagtgga agggattgtg acattaagtg
     1381 acgtcactga aaccattgcc ggtaacttac cgaacgaagt ggaagagatc gacgcccgcc
     1441 atgatattca gaagaatgcg gacggttcct ggacggcgaa tggtcatatg ccgctggaag
     1501 atctggtgca atatgtgccg ctaccgctgg atgaaaaacg tgaatatcac accattgccg
     1561 ggctgttgat ggaatattta cagcgtattc caaaacctgg cgaagaagtt caggtggggg
     1621 attatttgct taaaacgttg caggtggaaa gccatcgcgt gcagaaggtg cagattattc
     1681 cgctacgtaa ggatggcgag atggagtacg aggtgtgatg gtatcggtca ttttacccga
     1741 tgctgcgtaa cgccttataa cttcagcaat gaagcgttgt ctgtggtaca tggacattac
     1801 agcgggggtg tgggtccggc tgtaaatcgc cgaatcgttt cctgtaggcc ggggcagccc
     1861 acatggatgt gggctgaggg cgcgttttac agggatgtta cctcgcgccc gtacccgata
     1921 gccgaaagga ataagatgag ggaaccgcgc cagcggcgat ttaccgccgg gagcccgggt
     1981 tgccaggatg gtggcgattg agccaccctg gcacgttcac cggcttgagc gttccatagt
     2041 agtgaggaac atcaagtgaa cggaacagcc ttcgatctta catattcaga atataatggt
     2101 gccgccgcga acgcctgatg cgacgcttta cgcgtcttat caggcctaca ggacaggtca
     2161 cctggtaacc tacatcttct ccagcaactt cttcacatct ttgccattgc gggaatcttt
     2221 attccgctcg gcccagtcat tcaggcgacg tttcgcttcg tcctgtagat gtttgcgcag
     2281 tatctgatcc acttgcagac tgtaattgag ttgctgccag ttgccataaa cccgcagcgg
     2341 tactggcgtt tctttgagga aatcaatcag tttgctttcc ccgttccagc cacccacgac
     2401 ccgaatatca aactgggtgt cgcaggtttg atctgccaga ttcaacatgc cttcccctgt
     2461 aagcgccagc acaggcgatt gaccttgcat gtcgtttaac gtcacgacgc catccttcaa
     2521 cgtcaaatcg gtggtaaagc ggtcaagacg cgtcacgtta tcgaagtttt cagcggcctt
     2581 cacatcacca ccattacgtt ctaccgcttg ctgaatcatc tgctggaagt tcatcccttc
     2641 catgcgcgta tcggccattt cgacatgcgc cgtcccctgc cagttgtggc ggaatgcgtc
     2701 ggcatctatg tcagcaccgg agaaatcacc agccagtgac atctttccgg tcaacgaaat
     2761 cggatagtta aacgccttca gaatggtgcc aatctcaacg ttttccagcc gtggctggaa
     2821 gtttatccgc ggatttattg atgtcgcgtc cagcgtgccc ggcagtgaaa cctgtccacc
     2881 gttaagtttg ccctgcagtt gagtaatttc cagcaaaccc gacttgttgg tcatttgcgt
     2941 ggcaacatct gtaaaattca ttccgcgcca gcgcacgtta ctggcctgca acaaaatatc
     3001 agccgtaaag ccttgcagtc cctgataggc cggttcatca atacgcgaag aaatgaccgg
     3061 gcgcggcaac gttgattggc tctgcccctg ctgcgcggca ccgttttcac cattcgcggt
     3121 ttcattaagc gggatgaggt tgtcaagatt cagttgcggg aattgcagtc tcagctgcca
     3181 ttccggtttc tctgtcagcg tgacctgtgc ttgcccgctc agcgtactat cattggcggt
     3241 caaactaatt tggttaaatg aaaggcgttt atgtgactcc tgccactggg cctggaaact
     3301 cccctgcccc tgaatacctt gttttggtaa atctgcaccc tgcaactgcc agttaatttg
     3361 ttcaatagcc gccgttaaat catgcggata atcagaagca tctaccgtac cgttaaggga
     3421 tatcgtgaga tcgcgctgat cgcgattaac ccgcccggaa aactcaaatg agccacgatg
     3481 ttggggatct tgttccattt gcaggcgaat attgcggatt gtcacctgct cgtcatcttc
     3541 atgctggaac accagaacgc tgtccgccac cttaagactg gatatatcaa acgaccatcc
     3601 gcgatcgtct gacagatccg gcaaggtatt gtcgcgcggt gcaaccggag cgtcttcact
     3661 gcgcaccgct tccgtctgcg gcgtcagttg gatcactgcc ccttttagca tcacctgctt
     3721 aacgctcagt tgatgactca gtagtggtaa aagcgccacg tccaggcgca tgttgtcggc
     3781 gcgaaccagt ggctggctcg cgccctgggc ggtgagagac attcgcccgg agaggatact
     3841 aagctgcggc cagacgtgcc aacgcagtgg cccgtcgagc tgcaattgat aaccgctacg
     3901 tgcagcaact tgcttgacca tatagtcgcg gaaatcattc ggattcacca gcaacactaa
     3961 cgcagataac ccggccacca gcacgaccag gagtatcatc agcgtcgtca gaaatcgtct
     4021 catggtatcc tcaatgggcc tgaattagtc tttatcgatt cggctggcta ccgcgccctg
     4081 ctggttgcga tatttcgcat cttcacggcg gttgtaaggt cgcgccgccg ggccggaaag
     4141 cggctcaaag ctcagcgcac caattaacat gcccggacgc agcgccagcg gcagcttacc
     4201 ggagttgtag aactccagca caatgcagcc agaccagccc ggatcgatgc ggtgcgcggt
     4261 gacgtgcacc atcagcccca gacgcgccaa agaggaacgc ccgtccagcc agcccaccag
     4321 atcggctggc agcgtcaccg actccagcgt caccgccagc gccagctctc ctgggtgaag
     4381 atagaacgcc tcgctttcat cgagaacgat ctcatcgctc atcacgcggt caagcgcggc
     4441 gctcacttca tctttgggac cgctcagatc gataaacgct gccgtgtgac cacggaaggt
     4501 acgaaattta ttgcccaggc gtacatccac cgtcgcgccg ttaatacgct ccactggcgg
     4561 acgtgggttg atcgacaaac ggccttcatc aagccaggct tcaatatctc ggtcacacag
     4621 acgcatggca ttttctcctt tcgcgcatca ctcccttaac gccaattacg tcaagggcat
     4681 accaggttat cactgaacgg tacacaattt atcaagctta ttcaaagaac tgacttattt
     4741 tcgctttcaa tatatcgatc gcgatgcggt ttttcccgcc gcgcggcacg ataatgtccg
     4801 catattgttt agaaggctca atgaattgca ggaacatcgg gcgcacggtt ttttgatatt
     4861 gcgccatcac ggaatccatt gaacgcccac gctcgttaac gtcacgcttg atgcggcgca
     4921 tcaggcagat atccagcggg gtatcaacga aaatggagaa gttaagttcg tcacgcaaac
     4981 gcgcatccgt cagcaacaaa atgccttcga gaatgatgac cttcttcggc tcaaccgtca
     5041 ccgtttcttt catacgcgta tgttcaacat agctgtaaac cggcaggtca attgccgagc
     5101 cgcgtttcaa cgcttgtaaa tgctcaagca gcaggctgtg atccatcgcg ctgggatggt
     5161 cgtagttggt cttaacgcgt tcttccatcg acagatggct ttgatctttg taatagcagt
     5221 cttcgggaat tacgccgatg tgttcatcac cgacttgctc acgtaattca cgataaaggg
     5281 tactggcaat aagactcttg ccggaagccg atgcgccagc gataccgata atgacgcact
     5341 gatgagactg atcagtcata tatttagcga cctgattaac ctggatgtta ggaagggggc
     5401 gacgaagcgc caaacgcggc aattataggg atttcatccg cctgatacca gtcgaatagc
     5461 gttgccgcgc gctcagagtt aattgttgac aaagaattcc cggtggcaaa ttacgttgat
     5521 cagttttata caaagtaaaa aatgttatac gcagttgcgc aaattatccg cctttacgtc
     5581 actttatgag caattcgcat ataaaatgta aaacttttgt actagcataa acacagaaac
     5641 gaatactggc gacctggcct tgcggataaa gcggtaatga gcaaacaatc acagcatgta
     5701 ttaattgccc tgccccaccc gctgcttcac ctggtcagtt taggtttagt ctcgtttatc
     5761 tttacccttt tctcgcttga gctttcgcag tttggcaccc aactcgcccc actgtggttc
     5821 ccgacgtcca tcatgatggt ggcgttttat cgccatgccg ggcgcatgtg gccgggaatt
     5881 gcgctgagct gttcgctggg aaatatcgcc gcatccatcc tgcttttttc caccagctcg
     5941 ctgaacatga cctggacgac catcaatatt gttgaagccg tggtcggggc agtgctgcta
     6001 cgtaaattgc tgccgtggta taaccccttg caaaatctgg ctgactggct gcgtctggca
     6061 ctcggcagcg ccattgttcc gcctctgttg gggggtgttt tggttgtcct gctgacgccc
     6121 ggagacgatc ctctcagggc atttttgata tgggtactgt cagaatccat cggcgctctg
     6181 gcactggtgc cgctgggatt gttatttaaa ccacactatc tgctgcgcca tcgcaaccca
     6241 aggttgcttt ttgagtcgct gctcacgtta gccatcacac tgacgttaag ctggctttcg
     6301 atgctgtatc tgccgtggcc ttttactttc attattgtgc tgttgatgtg gagcgccgtg
     6361 cgcctgccac gaatggaagc ctttttgatc ttccttacca cggtgatgat ggtgtcgctg
     6421 atgatggccg cggatccctc cctgcttgct acgccgcgta cgtacctgat gagccatatg
     6481 ccgtggctac cgtttttgct gatcctgctg cccgccaaca tcatgacgat ggtgatgtat
     6541 gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa cccgctttcg gaacgcgatg
     6601 gaatattccg ctatcggtat ggcgttagtg ggcaccgagg gacaatggct gcaatccaac
     6661 aaagcactct gccagtttct cggttacagt caggaagagc tgcgcggact cacctttcag
     6721 caactgacct ggccggagga tctcaataaa gatctccaac aggttgaaaa gctgataagc
     6781 ggtgaaataa acacctattc aatggaaaaa cgttactaca accgcaatgg cgatgttgtc
     6841 tgggcgttgc ttgccgtctc tctggtgcgc cacacggatg gcacgccgct ctattttatc
     6901 gctcagattg aagacattaa cgagctaaaa cgcaccgaac aggtgaatca gcaactgatg
     6961 gagcgcatca cgctggctaa cgaagcgggc gggattggca tctgggagtg ggagctgaag
     7021 ccgaatattt ttagctggga taagcggatg ttcgaactgt atgaaattcc tccgcatatc
     7081 aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg aagatcgtca acacgccgaa
     7141 aaagtgattc gtgattcgtt gcaatcacgc tcgcccttta agctggaatt tcgcattgcc
     7201 gtgaaagatg gcatccgcca tatccgcgcc ctcgccaacc gggtactgaa taaagaaggc
     7261 gaagtcgaac gcctgctcgg cattaatatg gatatgaccg aggttaaaca gcttaacgag
     7321 gcattgtttc aggaaaaaga gcgcctgcac attacgctgg actccattgg cgaagccgta
     7381 gtctgtattg atatggcgat gaaaattacc tttatgaatc cggtggcgga gaagatgagc
     7441 ggctggacgc aggaagaagc attaggtgtt ccgctcctga cggtgttgca tattactttt
     7501 ggcgacaacg ggccattaat ggagaatatt tacagtgccg acacctcacg ttccgcgatc
     7561 gaacaagatg tggtgttgca ctgtcggagc ggcggcagct acgacgtgca ttacagtatt
     7621 acgccgttaa gtactctgga cggcagcaat attggttcgg ttctggtgat tcaggacgtt
     7681 accgaatcac gcaaaatgct gcgccagctg agctacagcg cctcccatga tgcactgacg
     7741 catctcgcca accgcgccag ttttgagaaa caactgcgta tcctgctgca aacggtaaac
     7801 agtacgcatc agcgacatgc actggtgttt atcgatcttg atcgctttaa agcggtgaat
     7861 gacagcgccg ggcatgcggc gggcgacgct ttgctgcgcg aactggcgtc gttgatgctg
     7921 agtatgctgc gctccagtga cgtgctggcg cggctcggcg gtgatgaatt tggtctgctg
     7981 ctgccagact gcaatgttga aagcgctcgt tttatcgcta cacgtattat cagtgctgtg
     8041 aatgactatc actttatctg ggaaggccgg gtacatcggg taggtgccag cgccgggatt
     8101 accttgatcg atgacaacaa tcatcaggcg gctgaagtga tgtcgcaggc tgatatcgcc
     8161 tgttatgcct ccaaaaatgg tggccggggc cgggtgacgg tttacgaacc gcagcaagct
     8221 gccgcacata gcgaacgggc ggcgatgtcg cttgatgaac agtggcggat gattaaagag
     8281 aatcagttga tgatgatcgc ccacggtgtc gcttcgccac ggatcccgga agcgcgtaat
     8341 ttgtggctga tttcacttaa gctctggagt tgcgaaggcg agattattga tgaacaaaca
     8401 tttcgtcgta gcttcagcga tccggcgctt agccatgctc ttgaccgccg ggtattccac
     8461 gaatttttcc agcaggccgc aaaagcggtt gccagtaaag gcataagcat cgctctcccc
     8521 ctttccgttg ccggtttgag tagcgccacg ctggtgaatg atctgcttga gcagctggaa
     8581 aatagccctc taccaccacg gttattacat ctgattattc cggctgaagc gattttagat
     8641 cacgcagaaa gcgtgcaaaa actgcggctg gcgggatgtc ggatagtgct cagccaggtg
     8701 ggccgcgatc tgcaaatctt caactcgctg aaagcgaata tggcagatta cctgctactt
     8761 gatggtgagt tatgcgccaa cgtgcagggt aatttgatgg atgagatgct gattacgatt
     8821 attcaggggc acgctcagcg actcgggatg aaaaccatcg ccgggccagt cgttttaccc
     8881 ttagtgatgg atacgctttc tggcatcggc gtcgatctga tttatggtga tgtgattgcc
     8941 gatgcccaac cgctggattt gctggtgaat agtagttatt tcgcgattaa ctgaggcgtg
     9001 cttccccatc gcctgatgcg acgctaacgc gtcttatcat gcctacaaat cgctcattcc
     9061 ccaggccgga taaggtgctc gcaccgcatc cggcgaccaa cgtcatgctt cgtctggttg
     9121 ccagccttcc gtataccaga tatgcaacag cgcataagaa cgccagggct tccagcgttc
     9181 ggcataacgg cggatttgtg ccggtgtcat tcccggaaat cgctgtttaa tcagataatc
     9241 atccggcagg aaaacatctt tcgcctgcca gccacgcaaa gcgaaataat tcgccgtcca
     9301 gcgcccgata cccggaaaag tttgcagcgt tttcatcgcc tgctccacat cgcccggaat
     9361 tgccattggt aagctgccct ccagcgccgc atttgccaga tgaatcagtg cctctgcccg
     9421 tttcaacgac atgcctaacg cttttaatgc ctgcgggtcg gctgctgcca gccgctgagg
     9481 cgtggggaag cagatatact ccggaaaatc atccagccgt tcgccataaa gctgtaccac
     9541 tttggcagtc aattttgccg ccatcgccac gctcaccagt tggcctaaaa tcgcccgcac
     9601 gccctgctca aaagcatcaa cactgccggg taaacgcaat ccgggccgcg ccgcgcctaa
     9661 cttgcccaac gcaccgttaa caatttgtgg gttacattgc agatcaaaca ggcggctcat
     9721 tttcgccaga cactctgcgg caacaggttc taaacctgca cttaaattta tgtgcagagt
     9781 atggcgggct atatccggaa tagcagtcac tacgccgcga tattcgccca ccgccagact
     9841 acgggcataa taactgtccg cgaccgtttc cacaccgctc accgcacggg cggcgagaaa
     9901 tcccaacatc cacgaccagt catacggcgg ctgccagttc agggtataca tcgcatctcc
     9961 ttattcatcc gctttcagca taaacgttat tcagacgctg cgctttgctt tcatattccg
    10021 gttgtcgcga cggcaacatt tcgctaaagt cacgcccctt cttcaccggc atggggatta
    10081 tttcgtgttt attggttttg attacggtac agcaaactgt tcagtggcgg tcatgcgtga
    10141 cggtaaaccg cacttgctaa aaatggaaaa cgacagcacg ctgctgcctt caatgctttg
    10201 cgcgccaacg cgtgaggcgg taagcgaatg gctgtaccgc catcatgatg ttccggcaga
    10261 cgacgatgaa acgcaggcgc tgttgcgtcg ggcgattcgt tataaccgcg aagaagatat
    10321 cgatgttacg gcgaaaagcg tgcagttcgg tctttcctca ctggcacagt acattgatga
    10381 tccggaagaa gtgtggtttg tgaaatcacc aaaatcgttc ctcggtgcca gcggcttaaa
    10441 accgcagcgg gtagcgctgt ttgaggatct ggtctgcgca atgatgttgc acattcgcca
    10501 gcaggcgcag gcgcagctgc cagaagcgat tactcaggcg gtgattggtc gcccgatcaa
    10561 cttccagggg ctgggcggcg atgaagcaaa cgcccaggcg caagggattc tggaacgcgc
    10621 ggcgaagcgt gccggattca gggacgtggt attccagtac gagccggtag cggctgggct
    10681 ggattacgaa gccaccttac aggaagaaaa acgggtactg gtggtggata tcggcggtgg
    10741 tacgactgac tgttcattgc tgctgatggg gccgcagtgg cgttcgcgtc tcgatcgtga
    10801 agccagcctg ctgggtcaca gtggttgccg tattggcggt aacgatctgg atatcgcact
    10861 ggcgtttaaa aacctgatgc cgttacttgg catgggtggc gaaaccgaaa aaggcatcgc
    10921 cctgccgatt ctgccgtggt ggaatgcggt tgccatcaac gacgttcctg cgcagagtga
    10981 tttctacagt agtgccaacg gtcgtctgct taacgatctg gtacgcgatg cccgcgaacc
    11041 ggaaaaagtg gccctgttac agaaagtctg gcgtcagcgt ttaagctatc gcctggtgcg
    11101 tagcgcagaa gagtgcaaaa ttgctctttc aagcgtagcg gaaacccgcg cctcactgcc
    11161 gtttatcagc gatgaactgg ctacgctgat tagccagcaa gggctggaaa gcgccctcag
    11221 ccagccactg gcgcggattc tggaacaggt gcaactggcc ctcgataacg cccaggaaaa
    11281 accggacgtt atctatctga ccggcggtag cgcccgttct ccgctgatta aaaaagcgct
    11341 ggaagaacag ttgccgggca ttccgattgc aggcggcgat gactttggct ccgtcaccgc
    11401 cgggctggca cgctgggcgg aagtggtgtt tcgttaattg aaaaatggct cagcagcggc
    11461 ttaaatctgc ctgcgcctga gccagttttt ctgccgcctg acgtaacagt tccatcaacg
    11521 gcatggtccg tgctggagca tgaaggcgga atcgctgcaa ctctgtcgcg ccgacagtca
    11581 acgccgattc cagccgatgt cgtttggcgg ccattttcgc cattaccgct tgtctgtctg
    11641 caggcgttac cgcttcgttc ggcctgaaca caaaacggcg ttcacagctc gctttccagt
    11701 cgatgaccgc ctgggtcaga tgatcaccaa accctttaac ttgcttaacc ccacgacggg
    11761 taacatccgc tgctgtttca ataccaaagg accgtaacgc cgctttacgc gcagggccaa
    11821 caccgggaat agaggcaaca tcaataaaaa atccctccag aaacttctgc ttctgccgtt
    11881 cccttgcagt gtcgtgaagt gctgccagag cacgtttttc ttcttccggt aacccgagca
    11941 tttcgtcctt cattttttcg agcatcgtcc gtttggcaat aaaaccttcc agcccaccca
    12001 actgttggat ctggctgact aaatggtcat agtccatttt cgtgcgatta aatgcctcac
    12061 ggcgttgctg gatttctgct ttataagctt tgcttgtcag actgccaata atccagatag
    12121 ccgccagcac aggaaccaaa ataatataac gcggttctgc ctgaaggccg caaagcagca
    12181 gcgataccgc tgacagtgcg atctcaagca gaatgatgta ttcgcgccgt aacaggccta
    12241 aaggaagcgg cctgccagtc ggttggaaat gatcgggtaa tggcaattgc actgctggcg
    12301 gtgctactga cgccatcacc atcgcccaga ctttcgccag cacaaaatcc ccgccggtgg
    12361 taataacctc ttcgccgaga tcaataaaat agataacgcc ttgattatcc agcgtacacc
    12421 acgggcagtc agtcaaatga gcggggtaaa catgcattgc tgaaacggta catttcttta
    12481 actgttggcg tagagagtcc agtgccgcta cccacgcttt tgccgtcgga cgccccgttg
    12541 ccacgccact ttccgtgaat gcctgctgaa acatggcttc aacatctccc ggtaacatcg
    12601 acagcggaat agatcgtggc ggcggtttta aaccacgtcg ctgattatct gacgcgtagg
    12661 cataacggaa atgggcaata tccgtctcca gcgcattacc cgcatcagag ataagcggca
    12721 caccagaata aggatgccgc ccaccaaaca agacgtgaaa aatcagcaac gcaaggccaa
    12781 aattatcgtg attcgcggta cgttcaaaac cgacaaatga tggcaaggtt tgtagctctg
    12841 gcggcgtaaa atgcgacacg ccgacttcgc ataaatgcag tgtgccattg gcgttaatct
    12901 gaaaggagtc actatcgatc aacaccactt tgctgtcgcg acctaccata aagctgttct
    12961 ggtttacgtc acccacgacg tgcccgtgct cgtgaaccgt agcaaaagat gaagcaatat
    13021 tgcgcgcaac atagagtaga aaatcccacg cacaatgagg gtaactctgg cgacgatgtg
    13081 ccgggctata gatcatatga atcggttctt taccagaaac ttttggcatc ataaaaccga
    13141 taacttttcc gcctcgtcca ccgtgaagcg ttgcctgcgg ccaggcgaca taattcaaca
    13201 actgcgcgtc agctgtcgca gccataaagg caagtttgtc ctgttttaag gcgggtggcg
    13261 gcgtgtgata aatcttggcg acgctatcgg caaactcatt gatatcataa accgcacctt
    13321 caccgccttt gcccagttca cggcctgtcg taacgcactc accagtagca gtatataaag
    13381 tgggtttcat ggtaaccaca gcgccagtgc caacgtttta tcatcatcgg tacgctcgtt
    13441 taccgccggg ctggacaaaa actgctttaa caattcaggt aataaatcga gctgttcctg
    13501 cgttgctgcc gccagtccat tgaaaaaagg ggtaaagaaa ggcacatgcg gagaattatc
    13561 caacatattt aacgccagtc gctggatacc atccgtaaat gctgcaactt tatgcacacg
    13621 ctcagtgctg gtgaaagttt ccagtctgga aacggcatct tcatcggtaa tgaaatgcgt
    13681 catattggca tattcacccg ccatcggcgt aagcggtaat tgcagaccgt gaccaaaatc
    13741 caccaccacg ccgccatcgc caatctgcat aatcaaggtg ccgttagccg atgaaatcaa
    13801 tcccagaaac gtgcaggcaa aatcgcgaac tgctaattct ttggcttccg cctcggcaaa
    13861 aagtcgctga cgaacggtca gcacaatatc cgtggcgagg atatcattga gtccaaattc
    13921 accgtgctgc actttttgcg acacataagc catcgcttca ttgatggcaa gcatcgcgcc
    13981 ttcgccaccc tgcgagacac tgcctgcgcc atcagcgaca aataccgaca ataatggctg
    14041 ctggtcattt agccaggcaa cctgcatctg gcaggcatcc tggcagggta aatcagcgct
    14101 aatgtgcgac gttccaacag cggatgcata aaccagacgc cagctcacac tgacgtccag
    14161 ccttttggcg cttccagtac aacttccgtt cccggcgtgg agcgggaaac cgaacgcagt
    14221 gaactggata accagctaaa cagttcgcgg aattgtaatc cctgcagaga caaaggctga
    14281 cgaacgctga tttgtgccag agtcttcata tccgccccct gaacaccgat ggaaaagaag
    14341 gcaaacttct tatcttcttc cccctgaaac actttgttgg cggcagcctg ccactcatcg
    14401 gttggagcgc catcggtgat caggaaaatc cacgggcgat aataggaaat accatttgcg
    14461 cggtactcac gtttccgctc ctctaccata tccagggctt tggtaatggc ggcacccatt
    14521 ggtgtatcgc cctgggcaaa caggatgggc gggaaaaaat tagcggcgct ggtaaagggt
    14581 tgttccacat gcaccgggcc gaacgtcaca ataccaagtt ccactctttt taaggccagc
    14641 gggtcggcaa gcagctcatc gcgaaaggta accaatccgg cgttaagttc attaatgggt
    14701 ctgccactca tcgaaccaga aacatccagc agcagaatgc aggggcaacg tggttccggg
    14761 ttactggcaa aatcgctggt ggcaaacgtg atttgttcgc tcataagaaa tcctttctta
    14821 gagaattatt gaggtccggt atcatactga ttgcaataat gaaataagag caaacgaaga
    14881 aactttcaga attagtcccg gctgcgcaga aaattttgct attcgcctcg caagaacgga
    14941 gagccgtcga ttgacactaa gggcggagtg acataatttc aggagtgagg gttagggaga
    15001 ggtttccccc tccccctggt gttcttagta agcctggaag ctaatcacta agagtatcac
    15061 cagtatgatg acgtgcttca tcataaccct ttccttatta aaagccctct tctccgggag
    15121 agggtttccc gtttcagcgt cccgctgaaa tcgtcggctt acctcctttc gccctgaatg
    15181 cagtctatcg ctaacgcgtg aacaaaatag ttccctctgt gattattgcg taacgactcg
    15241 caaagaatat ttgctggcag gatcgcagac tgcaaagcct gcagattgac aatcttatcg
    15301 tgaaggcata ctttcaggag taagggtaga gcggggtttc ccccgccctg gtagtcttag
    15361 taagccggga agcttatgac taagagcacc acgatgatga tccacttcat catgaccctt
    15421 tccttattta aggccccttc ctcgggaggg gctttcccgt ttcagcgtcc cgctgaaatc
    15481 gtcggcttac ctcctttcgc cctgaattca gtctatcgct aacgcgtgaa caaaatagtt
    15541 tcctctgtga ttattgcgta acgtctcgca aagaatattt gctggcagga tcgcagactg
    15601 caaagcctgc ggattgacat tctgaacgtg aaggcatact ttcaggagtg agggtagagc
    15661 ggggtttccc ccgccctggt agttttagta tgctgggtag ctaatcacta agagcaccac
    15721 gatgatgagt atcttcatca tgaccctttc cttatttaag accccttcct cgggaggggc
    15781 tttcccgttt cagcatcccg ctgaaatcgt cggcttacct cctttcgcca tgcatgcagt
    15841 ctatcgctaa cgcgtagata aaatagtttc ctgtgttatt actggatgcg tgctcgcaaa
    15901 tgtgcccgcc attcagacga ttcccgacag tgtttcataa ttcctccatt tttctccctt
    15961 attggctggc tacactagta tcattccgcg aaacgtttca ggaagagaaa ctcttaacga
    16021 tgaaaggcag ttataaatcc cgttgggtaa tcgtaatcgt ggtggttatc gccgccatcg
    16081 ccgcattctg gttctggcaa ggccgcaatg aaccccagag cgcagcacca ggtgcgacga
    16141 aacaagcgca gcacccacct gcgggggggc gccgtggtat gcgttctggc cctttagcgc
    16201 ctgttcaggc agcgactgcc gtggaacagg cggttccgcg ttatctcacc gggcttggca
    16261 ccatcaccgc ggctaacacc gttacggtgc gcagtcgcgt ggacggccaa ctgatggcgt
    16321 tgcatttcca ggaaggccag caggtcaaag caggcgattt actggcagaa attgacccca
    16381 gccagtttaa agttgcatta gcacaagccc aggggcaact ggcaaaagat aaagccacgc
    16441 ttgccaatgc ccgccgtgat ctggcgcgtt atcagcaact ggcaaaaacc aatctcgtat
    16501 ctcgtcagga actggatgcc caacaggcgc tggtcagtga aaccgaaggc accattaaag
    16561 ccgatgaagc gagcgtcgcc agcgcacaac tgcaactcga ctggagccgg attactgcgc
    16621 cagtcgatgg tcgtgttggc ctgaagcagg ttgatgttgg taaccaaatc tccagtggcg
    16681 acacgaccgg gattgttgtg atcacccaga cgcatcctat cgatttgctc tttactctgc
    16741 cggaaagcga tatcgctacc gtagtgcagg cgcaaaaagc cggaaaaccg ctggtggtag
    16801 aagcctggga tcgcaccaac tcgaagaaat taagcgaagg cacgctgtta agtctggata
    16861 accaaatcga tgccactacc ggtacgatta aagtgaaagc acgctttaat aatcaggatg
    16921 atgcgctgtt tcccaatcag tttgttaacg cgcgcatgtt agtcgacacc gaacaaaacg
    16981 ccgtggtgat ccccaccgcc gccctgcaaa tgggcaacga aggccatttt gtctgggtac
    17041 tgaatagcga aaacaaagtc agcaaacacc tggtgacgcc tggcattcag gacagccaga
    17101 aagtggtgat ccgcgcaggt atttctgcgg gcgatcgcgt ggtgacagac ggcattgatc
    17161 gcctgaccga aggggcgaaa gtggaagtgg tagaagccca gagcgccacc gcaccggaag
    17221 agaaagccac cagccgcgaa tacgcgaaaa aaggagcacg ctcctgatgc aggtgttacc
    17281 cccgagcagc acaggcggcc cgtcgcgcct gtttattatg cgtcctgtgg ccaccacgct
    17341 gctgatggtg gcgatcttac tcgccgggat tatcggttat cgcgccctgc ccgtttcggc
    17401 gctgccggaa gtggactatc cgaccattca ggtggtcacg ctctacccag gtgcgagccc
    17461 ggatgtcatg acctctgccg ttaccgcgcc gctcgaacgc cagttcgggc agatgtctgg
    17521 tctgaaacag atgtcgtcgc aaagttccgg cggtgcgtca gttatcactt tgcagttcca
    17581 gctaacattg ccgctggatg tcgccgagca ggaagttcag gccgcgatta acgccgccac
    17641 taacctgcta cctagcgatc tgcctaaccc gccggtttac agcaaagtga acccggcaga
    17701 tccgccgatt atgacgctcg ccgtcacctc aaccgccatg ccgatgaccc aggtagaaga
    17761 tatggtggaa acccgcgtcg cgcagaagat ctcgcagatt tccggcgtcg gcctggtgac
    17821 actttccggc ggccagcgtc cggctgttcg cgtcaaactt aacgctcagg cgattgccgc
    17881 cctcggcctg accagcgaaa ccgtgcgcac cgccattacc ggtgctaacg ttaactcggc
    17941 aaaaggtagc ctcgacggcc cttcccgcgc ggtcacgctt tccgcgaatg accagatgca
    18001 atccgccgaa gagtatcgcc agttgattat cgcctaccag aacggcgcgc cgattcgtct
    18061 gggcgatgtc gcaaccgtag agcaaggggc agaaaacagc tggctcggcg cgtgggcgaa
    18121 caaagaacag gccattgtga tgaatgttca gcgccagccc ggtgctaaca ttatctccac
    18181 cgccgacagc atccggcaga tgctgccgca gcttaccgag agtctgccga aatcggtgaa
    18241 ggtgacggtg ctttctgacc gcaccaccaa tatccgagca tccgtcgacg atacccagtt
    18301 tgaattgatg atggctatcg cgctggtagt catgattatc tacctgtttt tgcgcaatat
    18361 tccggcgacc atcattcccg gtgtcgctgt gccgctgtca ttaatcggca ccttcgcggt
    18421 tatggtgttt ctcgattttt caatcaataa cctgacgctg atggcgttaa ctatcgccac
    18481 cgggttcgtg gtcgatgacg ccatcgtagt tatcgaaaat atttcccgct atatcgaaaa
    18541 aggcgaaaaa ccgttggcgg cggcgctcaa aggcgcgggt gaaatcggct ttaccattat
    18601 ctcgctgacc ttctcactga ttgcggtgtt gatcccactg ctgtttatgg gcgatatcgt
    18661 cgggcgactg ttccgcgaat ttgctattac cctggcggta gcgattttaa tctcagccgt
    18721 ggtgtcgcta accctgacac cgatgatgtg cgcacggatg ctcagccagg agtcgttgcg
    18781 taaacagaac cgcttctccc gtgcctcgga aaaaatattc gacaggataa tcgccgccta
    18841 tggtcgtgga ctggcgaaag tgctgaatca tccgtggctg accttaagcg tggcgctcag
    18901 cacgctgctg cttagcgttc tattgtgggt gttcattccg aaaggtttct tcccggtaca
    18961 ggataacggc attattcagg gcactttgca ggcaccgcaa tccagctcct ttgccaatat
    19021 ggcccagcgg caacgccagg tcgcggacgt gattttgcag gatccggcag tgcaaagtct
    19081 gacctcattt gttggcgttg atggcactaa cccgtcgctg aacagtgcgc gtttgcagat
    19141 caacctcaaa ccgttggatg aacgtgatga tcgggtgcaa aaagtcatcg cccgtctgca
    19201 aacggcggtg gataaagtgc cgggcgtcga tctcttcctg caaccaacgc aggacctgac
    19261 catcgatact caggtcagcc gcacccagta ccagtttacc ttgcaggcca cgtcactgga
    19321 tgcgctcagt acctgggtgc cacagttgat ggaaaaactc cagcagctgc cacagctttc
    19381 tgatgtctcc agcgactggc aggacaaagg gctggtggcg tatgtcaatg ttgatcgcga
    19441 cagcgccagc cgtctgggga tcagcatggc ggacgtcgat aacgccctgt acaacgcgtt
    19501 tggtcagcgg ctgatttcca ctatttatac tcaggctaac cagtatcgcg tggtgctgga
    19561 gcacaacacc gaaaataccc caggcctcgc ggcgctggat accattcgcc tgaccagcag
    19621 cgacggcggc gtggtgccgc taagctcaat tgccaaaatt gagcagcgtt ttgcgccgct
    19681 ttccatcaac catctggatc agttcccggt aacgaccatc tcctttaacg tgccggataa
    19741 ctattcgctg ggcgatgcgg tgcaggcaat tatggacacc gagaagacgc tgaatctccc
    19801 ggtggatatc accacgcaat tccagggcag caccctcgcc ttccagtcgg cgctgggcag
    19861 cactgtctgg ctgattgtcg cggcggtggt ggcgatgtat atcgtgctcg gcattctgta
    19921 cgagagcttt attcacccga tcaccattct ctcgacgcta cccaccgcag gggtcggcgc
    19981 actgctggca ttaatgcttg ccggaagtga gctggatgtg attgcgatta tcggcattat
    20041 tttgctgatc ggtatcgtga agaagaacgc catcatgatg atcgacttcg cgctggctgc
    20101 tgagcgcgag caaggcatgt cgccgcgcga tgcaatctac caggcttgtc tgttgcgttt
    20161 tcgtccgatc ctgatgacca ccctggcggc tctgcttggc gcgctgccgc tgatgttgag
    20221 taccggggtc ggcgcggaac tgcgtcgtcc gttaggtatc ggcatggtcg gcggcctgat
    20281 tgtcagccag gtgctgacgc tgttcaccac gccggtgatt tatctgctgt ttgaccgcct
    20341 ggcattgtgg accaaaagtc gctttgctcg ccatgaagag gaggcgtaag tgaagttttt
    20401 tgccctcttc atttaccgcc cggtggcgac gattttactg tcggttgcca ttaccctgtg
    20461 cggcatactg ggtttccgta tgctgccggt cgccccgctg ccgcaggtcg attttccggt
    20521 gattatggtc agcgcctcgc tgcccggtgc atcgccggag acaatggcgt cttccgttgc
    20581 cacgccgctg gagcgctcac ttgggcgcat tgccggggtc agtgaaatga cctccagtag
    20641 ttcgctcggc agcacgcgca ttattttgca gtttgatttt gaccgggata tcaacggcgc
    20701 agcgcgtgat gtgcaggcgg cgatcaacgc tgcacaaagt ttgctgccca gtgggatgcc
    20761 cagccgtccg acctatcgca aagcgaaccc gtcggatgcg ccaattatga tcctgacgct
    20821 aacctccgat acttattcgc agggtgaatt gtacgatttc gcctcgacgc agctggctcc
    20881 gacgatttcg caaatcgacg gcgtcggtga tgtcgatgtc ggcggcagct cgctgcccgc
    20941 cgtgcgcgtc gggctgaacc cgcaggcgct gtttaatcag ggcgtgtcgc tggacgacgt
    21001 acgcactgcc atcagcaatg ccaacgtgcg taaaccgcag ggggcgctgg aagatggcac
    21061 tcaccgctgg cagatccaga ccaacgatga actgaaaacc gccgctgaat atcagccgct
    21121 gattattcac tacaacaacg gcggcgcggt tcgtctgggc gatgtggcga cggtgaccga
    21181 ctcagtgcag gatgtgcgca acgccgggat gaccaacgcc aaaccggcta ttttgctgat
    21241 gatccgcaag ctgccggaag ccaatattat ccagacggtt gacagcatcc gggcaaaatt
    21301 accggagttg caggaaacca ttccggcggc gattgatctg caaattgctc aggatcgctc
    21361 ccccaccatt cgcgcctcgc tggaagaagt cgagcaaacg ctgattatct cggtggcgct
    21421 ggtgattctg gtggtctttt tattcctgcg ctcgggtcgc gccactatta ttcccgccgt
    21481 tgcggtgccg gtttcgctga ttggtacgtt tgcggcgatg tacctgtgcg gattcagtct
    21541 caataacctg tcgttaatgg cgctcaccat cgctactggt ttcgtggtgg atgacgccat
    21601 cgtggtgctg gaaaacattg cccgtcatct ggaagcgggg atgaaaccac tacaggccgc
    21661 gctacaaggt actcgcgaag tcggctttac ggtgctgtcg atgagtctgt cactggtggc
    21721 ggtgttccta ccgctgctgt tgatgggcgg attgccgggc cgactgttac gcgagtttgc
    21781 cgtgacgctt tctgtcgcca ttggtatttc gttgctggtt tctctgacat taacgccaat
    21841 gatgtgtggc tggatgctga aagccagcaa gccgcgcgag caaaagcgac tgcgtggttt
    21901 tggtcgcatg ttggtagccc tgcaacaagg ctatggtaag tcactgaaat gggtgctcaa
    21961 tcatacccgt ctggtgggcg tggtgctgct tggcaccatt gctttgaata tctggctata
    22021 tatctcgatc ccgaaaacct tcttcccgga gcaggacact ggagtgttga tgggcgggat
    22081 acaggcggat cagagtattt cgtttcaggc gatgcgcggt aagttgcagg attttatgaa
    22141 aattatccgt gacgatccgg cagtggataa tgtcaccggc tttaccggcg gttcacgagt
    22201 taacagtggg atgatgttta tcaccctcaa gccacgcgac gaacgcagcg aaacggcgca
    22261 gcaaattatc gaccgtctgc gggtgaagct ggcgaaagaa ccgggggcga atctgttcct
    22321 gatggcggta caggatattc gcgttggcgg acgtcagtct aacgccagtt atcagtacac
    22381 gttgttatcc gacgacctgg cggcactgcg cgaatgggag ccgaaaatcc gcaaaaaact
    22441 ggcgacattg ccggaactgg cggacgtgaa ctccgaccag caggataacg gcgcggagat
    22501 gaatctggtt tacgaccgcg acaccatggc acggctggga atcgacgtac aggccgccaa
    22561 cagcctgtta aataacgcct tcggtcagcg gcaaatctcg accatttacc agccgatgaa
    22621 ccagtacaaa gtggtgatgg aagtggatcc gcgctatacc caggacatca gcgcgctgga
    22681 aaaaatgttc gttatcaata acgaaggaaa agcgatcccg ctgtcgtatt tcgctaaatg
    22741 gcaaccggcg aatgccccac tatcggtgaa tcatcaggga ttatcggcag cctcgaccat
    22801 ctcgttcaac ctgccgaccg gaaaatcgct ctcggacgcc agtgcggcga tcgatcgcgc
    22861 aatgacccag cttggtgtgc cttcgacggt gcgcggcagt tttgccggca cggcgcaggt
    22921 gttccaggag acgatgaact cacaggtgat cctgattatc gccgccatcg ccacggtgta
    22981 tatcgtgctg ggtatccttt acgagagtta tgtacatccg ctgacgattc tctccaccct
    23041 gccctcggcg ggcgttgggg cgctgctggc gctggagctg ttcaatgccc cgttcagcct
    23101 aatcgccctg atagggatca tgctattaat cggcattgtg aagaaaaacg ccattatgat
    23161 ggtcgatttt gcgcttgaag cccaacggca cggtaacctg acgccgcagg aagctatttt
    23221 ccaggcctgt ctgctgcgtt ttcgcccgat tatgatgact accctggcgg cgctgtttgg
    23281 cgcgttgccg ctggtgttgt cgggcggcga cggctcggag ctgcgccaac ccctggggat
    23341 caccattgtc ggcggtctgg taatgagcca gctcctgacg ctgtatacca cgccggtggt
    23401 gtatctcttt ttcgaccgtc tacggctgcg tttttcgcgt aaacctaaac aaacggtaac
    23461 cgagtaaatg acagatcttc ccgacagcac ccgctggcaa ttgtggattg tggctttcgg
    23521 cttctttatg cagtcgctgg acaccaccat cgttaacacc gcgcttccct caatggcgca
    23581 aagcctgggg gaaagtccgt tgcatatgca catggttatt gtctcttatg tgctgaccgt
    23641 ggcggtgatg ctgcctgcca gcggctggct ggcggacaaa gtcggcgtgc gcaatatttt
    23701 ctttaccgct atcgtgctgt ttactctcgg ttcactgttt tgcgcgcttt ccggcacgct
    23761 gaacgaactg ttgctggcac gcgcgttaca gggcgttggc ggtgcgatga tggtgccgat
    23821 cggaaggctt acggtgatga aaatcgtacc gcgcgagcaa tatatggcgg cgatgacctt
    23881 tgtcacgtta cccggtcagg tcggtccgct gctcggtccg gcgcttggcg gcctgctggt
    23941 ggagtacgcg tcgtggcact ggatcttttt gatcaacatt ccggtgggaa ttattggtgc
    24001 gatcgccaca ttgatgttaa tgccgaacta taccatgcag acgcggcgct ttgatctctc
    24061 cggctttctg ttgctggcgg ttggcatggc ggtattaact ctggcgctgg acggcagtaa
    24121 aggtacaggt ttatcgccgc tggcgattgc tggcctggtc gcagttggcg tggtggcact
    24181 ggtgctttat ctgctgcacg ccagaaataa caaccgcgct cttttcagtc tgaaactgtt
    24241 ccgcactcgt accttttcgc tgggcctggc ggggagcttt gccggacgta tcggtagcgg
    24301 catgttgccc tttatgacgc cggttttcct gcaaattggc cttggcttct caccgtttca
    24361 tgccggactg atgatgatcc cgatggtgct tggcagcatg ggaatgaagc gaattgtggt
    24421 acaggtggtg aatcgctttg gttatcgtcg ggtactggta gcgaccacgc tgggtctgtc
    24481 gctggtcacc ctgttgttta tgactaccgc tctgttgggc tggtactacg ttttgccgtt
    24541 cgtcctgttt ttacaaggga tggtcaactc gacccgtttc tcctccatga acaccctgac
    24601 gctgaaagat ctcccggaca atctggcgag cagcggcaac agtctgctgt cgatgattat
    24661 gcaattgtcg atgagtatcg gcgtcaccat cgccgggctg ttgctgggac tttttggttc
    24721 gcagcatgtc agcgtcgaca gcggcaccac acaaaccgtc tttatgtaca cctggcttag
    24781 catggcgttt atcatcgccc ttccggcgtt catctttgcc agagtgccga acgatacgca
    24841 tcaaaatgta gctatttcgc ggcgaaaaag gagcgcgcaa tgaagttctg gcgacccggt
    24901 attaccggca aactgtttct ggcgattttc gccacctgca ttgtcttgct gatcagtatg
    24961 cactgggcgg tgcgtattag ttttgagcgt ggctttattg attacatcaa gcatggtaat
    25021 gaacagcgat tacaactgtt aagtgatgcg cttggcgagc agtatgcgca gcatggcaac
    25081 tggcgcttcc tgcgcaacaa tgatcgcttt gtctttcaga tcctgcgttc atttgaacac
    25141 gataattcgg aagataaacc cggcccgggt atgccaccgc acggctggcg tacccagttc
    25201 tgggtggttg atcaaaacaa caaagtgctg gttggtccgc gagcgccgat tccacctgac
    25261 ggtacacggc gacccattct ggtcaacggt gcggaagttg gcgcggtgat cgcctccccc
    25321 gttgagcggt taacgcgcaa tactgatatc aatttcgata aacaacagcg gcaaaccagc
    25381 tggctgattg tcgccctggc aacgttactc gcggcgctcg ccacctttct gctggcgcgt
    25441 ggtcttttgg ccccggtcaa acggctggtg gacggcacgc acaaactggc ggcgggcgat
    25501 ttcactaccc gcgtaacgcc caccagtgaa gatgaactgg gcaaactggc gcaagacttc
    25561 aaccagcttg ccagcacgct ggagaaaaac cagcaaatgc ggcgcgattt tatggccgat
    25621 atttctcacg aactgcgtac gccattagcg gtgctgcgcg gtgaactaga agctattcag
    25681 gatggcgtgc gcaaattcac gccggagacg gtggcgtctt tacaggcgga ggtcggtaca
    25741 ctgaccaaac tggttgacga tcttcatcag ttgtcgatgt ctgatgaagg cgctctcgcc
    25801 tatcaaaaag caccggtaga tttgatccca ctgctggaag tggcgggcgg cgcatttcgc
    25861 gaacgattcg ccagccgtgg cctgaaactg caattttccc tgccagacag tattaccgta
    25921 tttggcgatc gcgaccgttt aatgcagttg ttcaataact tactggaaaa cagcctgcgc
    25981 tacactgaca gcggcggcag cctgaaaatc tctgccgagc agcacgacaa aacggtgcgc
    26041 ctgacctttg ccgacagtgc gccaggtgtc agtgacgatc agctacaaaa attgtttgaa
    26101 cgtttttatc gcaccgaagg ttcccgcaac cgtgccagcg gcggttccgg gctggggctg
    26161 gcgatttgcc tgaacattgt tgaagcacat aatggccgca ttattgccgc ccattcgcct
    26221 tttggcgggg taagcattac agtagagtta ccgctggaac gggatttaca gagagaagta
    26281 tgaccgagtt accaatcgac gaaaacacac cgcgtatttt gatcgtggaa gatgaaccga
    26341 agctggggca gttgctcatt gattatctgc gtgctgcgag ctatgcgccg acgcttatca
    26401 gtcacggcga tcaggtactg ccgtatgtgc gccagacacc accggatctg atcctgttgg
    26461 atctgatgct ccctggcacc gatggcctga cgctgtgccg ggaaattcgt cgtttttctg
    26521 acattcctat cgtgatggtg acagcaaaaa tcgaagagat cgaccgcctg ctggggctgg
    26581 agattggcgc agatgactac atctgcaagc cgtacagccc acgggaagtg gtagcgcgtg
    26641 tcaaaaccat tttgcgccgt tgcaaaccgc agcgtgagtt gcaacaacag gatgctgaaa
    26701 gcccgttgat tatcgacgaa ggccgttttc aggcttcatg gcgcggtaaa atgcttgacc
    26761 tgacgcctgc ggaatttcgt ctgctgaaaa cgctctctca tgaaccagga aaagtgttct
    26821 cccgcgagca attgctcaat catctttatg acgactaccg cgtagtaacc gaccgcacca
    26881 tcgacagcca cattaaaaac ctgcgccgca agctggaatc tctcgacgcc gaacagtcat
    26941 ttatccgcgc cgtttacggc gttggttacc gctgggaagc cgacgcctgc cgcatcgttt
    27001 agttttagcg acatgacttt gttagccgga gatggcgttc agacccgatc cggcaacatt
    27061 atcccacgca tggtcagcaa actgacatta tctctccgcc ctggcaggca tcagaaaatc
    27121 gctcatactt taatcggtaa acagcacctt tagatgctgt tttcgataca caatttcaat
    27181 caaggagtca ttatggctgg ttggtttgaa ctcagtaaga gcagtgataa tcagttccgg
    27241 tttgtgctaa aagcgggcaa tggtgagact atcctcacca gcgagctgta tacttcaaaa
    27301 gcctctgcgg aaaagggcat cgcgtcggtg cgtagcaaca gcccgcaaga agaacgctat
    27361 gagaaaaaaa cggcaagcaa cggcaaattc tatttcaatc tgaaagccgc caatcatcaa
    27421 attatcggct ccagccagat gtatgccacc gcgcaatctc gtgaaaccgg aattgcctcc
    27481 gttaaagcca atggcacaag ccagacggtg aaagacaata cgtaattacg atgccgggcg
    27541 tgggttgttg gcgtccggct ttatttccaa ataacgctta tttatttacc tgcgccatta
    27601 aaatagtgga cactgatgaa tactcattgt gctttctttg tttaacatct ccatgcttat
    27661 cctcctgaac atgttcatct gccaaaatat cttctaatac ttttcgtgac gctatccggt
    27721 ttatgacata cactgatgta agatttttac ttacaggaga gcataaacat ggccagaacg
    27781 atgacggttg atctcggcga tgagttgcgt gaatttatcg agtccttaat tgaatccggc
    27841 gactatcgca cccaaagcga agtgatccgt gagtcactgc gcttacttcg agaaaagcaa
    27901 gcagagtctc gcctgcaagc attacgtgac atgcttgcag aagggttgag tagtggtgaa
    27961 gctcagccgt gggaaaagga cgcgttttta cggaaggtga aagcagggat aaggaaataa
    28021 tgtgcgtatt attaaactca tgccaaaagc caatgaggat ttagaaggca tctggtatta
    28081 tagttatcac catttcggcg aaccacaagc cgacagatat gtggaacatc tttcagacgt
    28141 tctccagatt ctgagtaaca ataatatagg tacgcccaga cctgaattag gcgaaggtat
    28201 ttttgttctt ccttttgaac gccacgtaat ttatttcctg caatcaccag gggaaattat
    28261 cgtcattcgt atccttaacc aaaaccagga cgccaccaga catttacact ggagctaaac
    28321 ccgccgattg cgctacaatg cccgccctta aagtgggggc actcccctaa ccgcttcatc
    28381 aggtgaagcg gatctgacct gtcatcagaa cgagagaatt atgtttaaac cggaactcct
    28441 ttccccggcg ggaacgctga aaaatatgcg ttacgctttc gcttatggcg cagatgctgt
    28501 ttatgcgggc cagccgcgtt actccctgcg tgtgcgcaac aacgaattca accacgaaaa
    28561 ccttcagctc ggcatcaatg aagcccacgc gctggggaaa aagttttatg tcgtggtcaa
    28621 cattgcaccg cacaacgcca agctgaaaac ctttatccgt gacctgaaac cagtggtgga
    28681 aatggggccg gatgcgctga ttatgtccga tccagggctg attatgctgg tgcgtgagca
    28741 cttccctgaa atgccgatcc atctctcggt acaggctaac gccgtgaact gggcgacggt
    28801 gaaattctgg cagcaaatgg gcctgacccg cgtgatcctc tctcgcgagc tgtcgctgga
    28861 agagattgaa gagatccgca atcaggtgcc ggatatggag atcgagatct tcgttcacgg
    28921 cgcactgtgc atggcctact ccggtcgctg cctgctctct ggctatatca acaagcgcga
    28981 tccgaaccag ggtacctgca ccaacgcctg ccgctgggag tacaacgtcc aggaagggaa
    29041 agaagatgac gttggcaaca tcgtacacaa gtacgagccg attccggtgc aaaatgttga
    29101 gccgacgctg ggtatcggcg cgccaaccga caaagtgttt atgatcgaag aagcccagcg
    29161 tccgggcgag tatatgaccg cgtttgaaga tgagcacggc acttacatca tgaactcgaa
    29221 agatctgcgc gctatcgccc atgttgaacg cctgaccaaa atgggcgtgc attcgctgaa
    29281 aatcgaaggc cgtaccaaat ctttctacta ttgcgcacgc accgcacagg tttaccgcaa
    29341 agctattgat gacgccgctg cgggcaaacc gtttgacacc agcctgctgg aaaccctgga
    29401 aggcctggcg catcgcggtt acaccgaagg attcctgcgt cgtcatactc acgacgatta
    29461 tcagaactac gaatacggtt attcggtttc tgaccgccag cagtttgttg gtgagtttac
    29521 cggtgagcgc aagggggacc tcgcggcggt agcggtgaaa aataaattct ccgttggcga
    29581 cagccttgag ctgatgacgc cgcaaggcaa cattaacttt acccttgagc acatggaaaa
    29641 cgccaaaggt gaagcgatgc cggtcgcacc aggcgatggt tatactgtgt ggctcccggt
    29701 gccgcaggat cttgagctca attacgcgct gctgatgcgt aatttctccg gggaaaccac
    29761 gcgtaacccc cacggtaagt gattaatttc gattattttt cccggatgga aaattcttag
    29821 aaaccgatca catacagctg catttattaa ggttatcatc cgtttcgctg aaaaacataa
    29881 cccataaaat gctagctgta ccaggaacca cctccttagc ctgtgtaatc tcccttacac
    29941 gggcttattt tttatgcata agccctatcc ctggtcaccg tcttccattg accacatcga
    30001 tagaatctcc catcatagca cgatgccttt cacgtaacgg catcgtgctc gcacaggttc
    30061 cggctacgca cagccagaac gcgcatgttt gacgcttacc aaaaaatatt ctcactctac
    30121 acatttgaat gtcagacgag cgacgccatg taatcctgca ccttctgtct tcaggtcaac
    30181 tatctgcatt tttttgccct gagtaacaca gaaatgggct gcatcatttt ttactatatt
    30241 ttctgcacca gatattctcc ccctggctaa agaagcttcg gcttcggtgt agtattggtt
    30301 atcgagttta cgctgaatat tacttttata tgcaagacca aatttaccga tacttgtctc
    30361 atcattatgc acagcacaac cagacataag aaaaatacta aataatgata tagcaactat
    30421 ctttttcatc tcacctttcc ccattaaata ccaacgacac tctctagtgt ttaaatata
//
LOCUS       DAZFKF010000055        29788 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFKF010000055 DAZFKF010000000
VERSION     DAZFKF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.055"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            660..1112
                     /locus_tag="ACHKA1_002940"
     CDS             660..1112
                     /locus_tag="ACHKA1_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiF/GrlA family transcriptional regulator"
                     /protein_id="HFO5680560.1"
                     /translation="MARKLKINPVIPECVADLKGYPLYIIVAYWGLRNNKTLTTTDVS
                     RNFLITQQQSYDVLTYIYQEAQKQITATKRTLFNEHKRRISAFRILDIDKGILRTPKK
                     EIAYASYDNIPTVTMKSTRCEIQDSFTELRRWMCSRKTGDRVEVFLNA"
     gene            1105..1299
                     /locus_tag="ACHKA1_002941"
     CDS             1105..1299
                     /locus_tag="ACHKA1_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO5680561.1"
                     /translation="MPKYQINATIKKPGGGPVKWTHYSDLPMTAEECRKNFSKEKEAG
                     KCFSVDVHISDFICKEIHPQ"
     gene            1345..2298
                     /locus_tag="ACHKA1_002942"
     CDS             1345..2298
                     /locus_tag="ACHKA1_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO5680562.1"
                     /translation="MVTSIPLLLLILVALIFVKSAVKIVPQGNAWTVERFGKYTHTLS
                     PGLHFLIPFMDRIGQRINMMETVLDVPKQEVISKDNANVTIDAVCFIQVIDAAKAAYE
                     VDNLASAISNLVMTNIRTVVGGMNLDDMLSQRDSINSKLLTVVDYATDPWGIKVTRIE
                     IRDVKPPEELTKAMNAQMKAERTKRAQILEAEGIRQSQILKAEGEKQSQILKAEGERQ
                     SAFLQSEARERQAEAEARATKLVSDAIAEGDVQSVNYFIAQKYTEALQAIGTASNSKL
                     VMMPLDSSSLVSSVAGISELLKNVSQDTPKVDMKQAQQYLS"
     gene            2360..2803
                     /locus_tag="ACHKA1_002943"
     CDS             2360..2803
                     /locus_tag="ACHKA1_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO5680563.1"
                     /translation="MFWFTLFLLCIALEIITGTGWLLVISLGALSSAIVDFFLPISQE
                     ANICFFASISILTSIIKFVYDKKHKKLDTPLVNTGHTRFKGKEFTLSDDIVNGKGQLL
                     IGDTFWPVETLHDENYPAGTRVIVTDMQGITLKVMTIEDKSENKE"
     gene            2807..2977
                     /locus_tag="ACHKA1_002944"
     CDS             2807..2977
                     /locus_tag="ACHKA1_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680564.1"
                     /translation="MHLSLLIPLTGAAMNALCIFIIRLLDKDFIEVYKKTLKKITALH
                     ILLMVLSVTGIV"
     gene            2988..3650
                     /locus_tag="ACHKA1_002945"
     CDS             2988..3650
                     /locus_tag="ACHKA1_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680565.1"
                     /translation="MRNANEGISLAAIVAKETTTGKWNGTGGGIGLGTGGIGLFIGGV
                     SGSKQEQTQRAKNFESPKEEEYSLLNVYGSIIKILVFGVLFGLSSRLFTFLSDTGSDF
                     AEPVTTTTATGKIQSILGGMAEMVSILGTVVPFLVIIIAMIWFLFFSSKREKEELTRV
                     EHEAAKTKLRIDVYHRLRYVESDHVVFDPITGREVPAERACINKLIEALVDESNDISK
                     RN"
     gene            3801..4394
                     /locus_tag="ACHKA1_002946"
     CDS             3801..4394
                     /locus_tag="ACHKA1_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680566.1"
                     /translation="MRNANEGVSLTEAILAIRGIDKYASYKTIKTIKTIIITIVLIIL
                     ALAFFSLVSSLVSAAGRVAVAIQAVCYLCLCMAIIIMVVIKISSFIDERREKLELKRA
                     ELDKIKIKARQKIYKRLRYVENDHIVFDPVTGREVPAKWACIHKVIDEIIADKSNNVI
                     MFDPVTGREFLADGCNNVKKVAAPLKDTMKCTEENIT"
     gene            4608..4910
                     /locus_tag="ACHKA1_002947"
     CDS             4608..4910
                     /locus_tag="ACHKA1_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680567.1"
                     /translation="MTQAKANLRKLRRARYVPPRTVKKSITNTERVVSKAITGCGVFR
                     DIPQVKINLIETRKILQIELALSIANLSEANPIEIRKKIKSIDRQLSSTSKIRTTL"
     gene            4907..5164
                     /locus_tag="ACHKA1_002948"
     CDS             4907..5164
                     /locus_tag="ACHKA1_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680568.1"
                     /translation="MKKNDDLITLPSHNSHALDYIIKKAFTPRKTKTSTATHNVYSYN
                     KEESTLNFQKIGEAIFFGTDLVIKLKPNISASGELFICQKD"
     gene            5168..6163
                     /locus_tag="ACHKA1_002949"
     CDS             5168..6163
                     /locus_tag="ACHKA1_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFO5680569.1"
                     /translation="MYIVSKTLELVTTIVESTAATNAAKVAQAISPTFFAAITLYVIY
                     LIYEITYAQRDVLMNEVTKNIGAFALVGAFTYSAPYYSQFVIPFVMHAGSDLSAAVTG
                     GSGTATTVDNLWDTLSTSLNDFKKTNLEILAWTDITEKLYIYLIWGVGYVGGLLLIYY
                     TAVFLALSTFMVGILLSAGILFICFSLFASTRNMFTAWVGSCLNYILLNLFYSISFSF
                     VIGFIEQTVPSSGNITLTTVVNFFMIIVISIFLVEQVGTLCSTLTGGVGINGLTSAAN
                     GFGGKVASGFMRASGLRSFTNGFASKMPNPIFNAGSRSASALVQGLNRGSKLKAG"
     gene            6169..6810
                     /locus_tag="ACHKA1_002950"
     CDS             6169..6810
                     /locus_tag="ACHKA1_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFO5680570.1"
                     /translation="MKDKVLSFLFAGIITVTSPSIFASGIPVVDVTAIAKTVEEGLNR
                     AAEAARQLEQLKQQYEQTIRYAEEQKKRLEGFTDFSNGFDSASSYMKDSLSTITNSAK
                     SDLSSLRSQYDLSSNVAETQKKYDAILAKIKFYENFNNEMQERAKRLTTLQKEFASAD
                     TPQKKADLSNQLNTEKLTMELQLKQYDIAERQLEAEQKAQHQAIVNQLMDRLT"
     gene            6813..7067
                     /locus_tag="ACHKA1_002951"
     CDS             6813..7067
                     /locus_tag="ACHKA1_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HFO5680571.1"
                     /translation="MINLRSAIIPLLMVSTTLILSGCKEKVYAVEYYSSNPAEAAKTI
                     EQCRKGEITDQNCDNARAALEQAQKEEHKKKVRDLMERLD"
     gene            complement(7120..7929)
                     /locus_tag="ACHKA1_002952"
     CDS             complement(7120..7929)
                     /locus_tag="ACHKA1_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5710 domain-containing protein"
                     /protein_id="HFO5680572.1"
                     /translation="MNEPLKAELHSLFSFDIYPGASSEQNTGVKLAMARFYLNVPFEE
                     KNLAKQKGAQWDQEQRKWFVPQGKNPIYFIRWIKELNEHDYNVFSQRFYIAESYQSCW
                     RCKKTTPVFGIFLPRWYKYRDVIWGVDPAEWEDCILDEWYETSSPKGMEYFDSKKNMI
                     YRWLTSRVWWTDLTKIEIISTSALSRINEYSKLYYPSHSKTAKMNYYANHCCHCNAMQ
                     GDFMMFNEPGGVFFPVTYEQAEKIRFHEVNETIFAKASYSLIPEAGGFIDL"
     gene            7977..8612
                     /locus_tag="ACHKA1_002953"
     CDS             7977..8612
                     /locus_tag="ACHKA1_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680573.1"
                     /translation="MKSIILSMVAATALLSGCADSKTQPVPESPLTKHDHQITLKIDT
                     VRTDLPDSSPVCLNGMNIYESLSERHPGLFAKNQNVIFRSAFFDDGQAISSETIRKIM
                     QSTPCVESQTTSAGSWEPATELIPRQAISGDTTLAEKDRTAGFTMRIAPEIERGNFHL
                     QLTTTDPIRKQTVEQNILLKPDQPLIVSGFTGVNADSQSENRIVIITPHVR"
     gene            8784..9071
                     /locus_tag="ACHKA1_002954"
     CDS             8784..9071
                     /locus_tag="ACHKA1_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFO5680574.1"
                     /translation="MLSMIAASAIALSAPVYASDPCASVLCLYGKAIGSGGGSECKSA
                     EKDFFNIIKKKKGSIRWSKTFDARKAFLNQCTTADPAAISKIMSKFGRSWG"
     gene            9074..10309
                     /locus_tag="ACHKA1_002955"
     CDS             9074..10309
                     /locus_tag="ACHKA1_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin co-regulated pilus biosynthesis Q family
                     protein"
                     /protein_id="HFO5680575.1"
                     /translation="MKKLIIIPTTLLLNACSHAPEPAQAKGGWYELNTTIEQVRADTY
                     YNGVILQQPVSQPNSTVQYSNDNYLKKEAINVTPAPRIAETVKPRSEQSATAPKNQGS
                     NNSFLKPTTNSITSSGEIKTAAKGATEQHSEQPALPPAKPISSNNHQAQTAQNTDQQT
                     KPRPEQPPLMSKSGFLKKNANNAVPATTLTGPSTPEKQIYFVRHDGKGSLQKAVKEIV
                     PSDWTAEISPEVTKTFRRTISWQGNDQWPYVLDKMLRNYGLTVIKNTEKRHLLIDFQQ
                     QKGKTSQKAPVSPDKTPASPVRTPVSSDKLPQKPLTPVTVPQKQTEKLTWNLKKGSTL
                     RDGLTEWASSSPCGNSTWNLVWDTPVNYRIDAPLIFRGNFESALKDTLMLYLSADKPL
                     YAFRNIAQCVITIKDTQQE"
     gene            10315..10752
                     /gene="pilM"
                     /locus_tag="ACHKA1_002956"
     CDS             10315..10752
                     /gene="pilM"
                     /locus_tag="ACHKA1_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFO5680576.1"
                     /translation="MGWFFSLLLIFLSIGSYYLTDNNNQTIQRMKLLPAKQEAIEFVH
                     FANVINDYLYKYPDKRNSGGTLTSEQIGITPVYDIHHIIYGKRVYIWSADTEGLMSAL
                     QQQTKHSAMLGRVKNKKIVDNQGNDMGVPVPSSIPEEAIVLIN"
     gene            10871..11269
                     /locus_tag="ACHKA1_002957"
     CDS             10871..11269
                     /locus_tag="ACHKA1_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680577.1"
                     /translation="MQDNDDVSELDIATREVLILRCNLIQLASEYIKINPLKNTRIYT
                     DLIHRSADVIKNIEQGNIQNKHNYLSQDVKHTLVLYDLYKSDFEQCPGAKSLIPVREK
                     IVSLKELLSGANELDSIVRRPRRKKRSSRI"
     gene            11290..11874
                     /locus_tag="ACHKA1_002958"
     CDS             11290..11874
                     /locus_tag="ACHKA1_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO5680578.1"
                     /translation="MQLSAAVLAALISQCAPDVSPDTMNALIMTESGANPYVIANVSD
                     GTSKYFKDEKGAIEYAEKLTAENKRFSAGLTQIYSKNFPSLNLTNKTVFDPCTNIKAG
                     AAVLTDNYLRQKEGSSNQKILRALSLYYSGNESTGFIKEKKFNNTSYLERVIRNANNY
                     IVPSIREKTDESEIKPPNDEEKTSPDWDVFGDFS"
     gene            11874..12164
                     /locus_tag="ACHKA1_002959"
     CDS             11874..12164
                     /locus_tag="ACHKA1_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HFO5680579.1"
                     /translation="MKVKSTLQYFILAFFLSLTASVAYAGGLDTATNTMTELKTWAFG
                     FGGVCALCYLIYNIIMAFMEKKSWSDVGMALIYCSLAGGALYAGNWALELFK"
     gene            12235..12555
                     /locus_tag="ACHKA1_002960"
     CDS             12235..12555
                     /locus_tag="ACHKA1_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFO5680580.1"
                     /translation="MDKVNEHVSESFMTYNGFNRPALVSGIPLMLLLFTAFFAVLTGF
                     PAIFLWGIKGIIIPVICALFLFIVKLACENDSNALRVIRLNLMGLLLKIRHRDLIIGY
                     SSVR"
     gene            12561..14918
                     /locus_tag="ACHKA1_002961"
     CDS             12561..14918
                     /locus_tag="ACHKA1_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFO5680581.1"
                     /translation="MHKIKLKNSDNMDVAREYPDYRFHITDSIIFTSDRKMLASLVVA
                     GIPFETENDNVLTNLFNSVKNFLIGLGKEGDLYLWTHLIKKRITIDGEWNFDNAFLSR
                     FSKKYLALFTSSAFYKSTWYLTFGIPYDDVDTGIERMNEMTQQAQKALQPFNASVLSV
                     HNTYISEVADYLSLLLNTEHNMIPLSSTPLSSSICDSEWYFGADVLELRNNESDSKKF
                     ATNYILKDFPIETTPGQWDFLLKQPYEFILTQSFIFESPTKTLKNIDSQLNKLQSAND
                     AAKTQQEELEAGKEAVAAGITLFGSLHCALTVFGDAPDQARSNGIKLSAEFITSGKGF
                     RFSRASLASPFVFFSHMPLNKRRPLDTRRTITNLACLMSFHNYSSGKKSGNPIGDGSA
                     IMPLKTVSDSIYWFNTHYSPPEKNVTGQKIAGHGMILGATGTGKTTFEAAASGFLQRF
                     DPLMFVVDFNRSTELFVRAYGGSYFTLQEGIYTGCNPWQLAEGPESPVWHRLLAFLKR
                     WTQVLARDNQGNPCSDEHGIELNAAVESIMRMPVEERRTALLLDIVSPELQTRLAKWC
                     DNGEYAWAVDSPRNTFNPLHHKKVGFDTTVVLDTKNGIHPACEPLLAVLFFYKEIMQR
                     GGNLMLSIIEEFWMPANFPMTQSMIKSALKAGRMKGEMMWLTSQSPEDAINCAIFAAL
                     VQQTATKILLPNPDAKWEGYKEIGLTEKEFEKLKELTKESRTMLIKQSGSSVFAKMDL
                     FGFDEFIPVLSGSETGLSIFDEIIAEKGDVAPDIWIPELLKRLNG"
     gene            15084..15818
                     /locus_tag="ACHKA1_002962"
     CDS             15084..15818
                     /locus_tag="ACHKA1_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HFO5680582.1"
                     /translation="MENTNYYEHSKKEATKKKFEEKNEKKDYFKAIRDFERSEIEIIK
                     KKAKTFTILAIGEFVVICILGFAIASLAPLKTAVPFLVRVDNSTGYTDIAPQLSDAKE
                     SYQDVETKYFLSKYLINYEAYDWQTIQEQADTVKTMSSQKVFSAYDTMIRADSSPLNI
                     LKNNYKIKVQINSVILLRKDMAQVRFKKMVLDLSGKPAPGYRATEWISTISFDWDKDI
                     KTEKERLVNPLGLQVLSYQPDPEVIK"
     gene            15884..16585
                     /locus_tag="ACHKA1_002963"
     CDS             15884..16585
                     /locus_tag="ACHKA1_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFO5680583.1"
                     /translation="MYGTPSERDGRIQTVDYNEQDVFNVRVKAGAQTTIKFGQDETIK
                     DVGIGDPEAWSVSVRDNTLFLRPKAEEPDTNVTVQTNKHIYPLYLISTTKQPTYILRF
                     NYPKPPSATVMTEKPFPCTDGGIINGHYQLKGDKSIFPYQVWDNGEFTCMRWTNKQEI
                     PVLYRVDADGNEHLVNGDRNKNTMVYYDVAENLRLRLGDQVADIRTSSIVNRPWNKKG
                     TSNGKTRVEKFSYEK"
     gene            16575..17714
                     /locus_tag="ACHKA1_002964"
     CDS             16575..17714
                     /locus_tag="ACHKA1_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFO5680584.1"
                     /translation="MKNKDDEKNNDAGNRGIIEVKGKAAPKKILILIILLIAALFVII
                     ILFKVLSREQVVQQTPLEKSDETLVTNTNNGVSLTTMMKNIEEKEKLDAANRRKAQED
                     QEQKQADNAPSAPASQKADQTAVNVIANGTPQTASGDPNQPQPLPKSVRQLMGDTMVK
                     IDNQEPGEKNQERDDLQGSQYADGKVSPVLNRRYLLSAGTALSCVLKTKIVTSYPGIT
                     MCQLTRDVWSDNGEVLLARKGALLIGEQNKVMTQGVARVFVNWTTLKDENVNVRIGAL
                     GTDSLGASGLPAWVDNHFGQRFGGALLLSLLGDGLDILKNSTQQTGSNSNITYENTSD
                     ATKEMAKTTLDNTINIPPTAYINQGTVLSVIVPRNIDFSSVYELQ"
     gene            17741..18796
                     /gene="virB11"
                     /locus_tag="ACHKA1_002965"
     CDS             17741..18796
                     /gene="virB11"
                     /locus_tag="ACHKA1_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFO5680585.1"
                     /translation="MNNIRDTSNTSQPRDNSKTARRYLDMTGIQSVLNIEHVTEISVN
                     KPGTIWFEGKNGWESKDAPDATFDNLMTLAKTLTNLSKIKIPLSHDNPIASVVLPGGE
                     RGQIIIPPATENNSVVISIRKPSLTRFTIDDYVRTGRFDNVRIATKHEAILTERQRYL
                     YELSRRPDGQSKAQFLREAVKDRLNFLIVGGTGSGKTTIAKAIADIFPPERRYITVED
                     VPEMSLPLHPNHIRLFYKKNTVEAKEIIEACMRLKPDHIFLAELRGNEAWSYLEALNT
                     GHEGSISTIHANNTYASFSRLASIVKQSDVGMTVDMDLIMRTIKTSIDVILFFNHTRM
                     TELYYEPEEKNRFLSTM"
     gene            18812..20770
                     /locus_tag="ACHKA1_002966"
     CDS             18812..20770
                     /locus_tag="ACHKA1_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFO5680586.1"
                     /translation="MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDISLLK
                     WDTFHSVISTFSGNPQYKKLVFMAWFGFSVPLIFFAIFMLIVVIGIMPKKVIYGDARL
                     ATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQFIYFAGQQFLILYAPTRSGKG
                     VGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAETIDQAIKG
                     QIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDK
                     ERFHLEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFF
                     FKFMSAPDRTRGSIETNFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPD
                     ALITHEKIVNLFFSLLVNENCRELPEHNPDLKYQCLILLDEFTSMGKSEVIERAVGFT
                     AGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLYYPPKSKNALAKKISEEIG
                     VRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNIAIREII
                     TSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKA
                     GGKKVMVAGGNVITNPDLDNHDKTDVSE"
     gene            20821..22464
                     /locus_tag="ACHKA1_002967"
     CDS             20821..22464
                     /locus_tag="ACHKA1_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFO5680587.1"
                     /translation="MNKKLLCLLISSFLLSGCANFKKINANELAAERDAQKATEYAKK
                     IRNNPVIQDSTKQWINTTPIIEKQVKQSAPPCPIVINTRSDIELRQIAQRITQTCHIP
                     VRISADVWTYLNGTGTGSTQQMTGSIPAPDANGMVPLASVGSQPVKVSSGGTSLPELN
                     ISGLPALLQTVSSRLGISWKYDKGRITFFYLETRSFPITYMDSNVAYNSKVVSGTMSS
                     SGSTGSTSSGGMTGDASNTQTTTVEMKSSLYNDLKSEVSSMLTPGTGRMYLSTGSLTV
                     TDTPDVLDSVQEIVNKRNNEMSRQVVLNVEILSIKKSSQEQAGIDWNAVFNDGHLGLS
                     LGGSFGDAAENVITSGVSIVDGKLAGSKAFLKALSSQGSVSVVTQNSAVTKNLTPVPM
                     QIANQQGFIESVTTDSTANVGSSTSLNAATITTGFNMTLLPYIQPDSQNLQLLFSMSL
                     SDKPTFEVFESGGSKAQTPNVDLKTINQTIDLKSGQTVILSGFQQSNRKTSKQGVITP
                     SFFGLGGGVNSEDGDTILVVLITPNVLNSGSSAVIKNVN"
     gene            22515..23825
                     /gene="pilO2"
                     /locus_tag="ACHKA1_002968"
     CDS             22515..23825
                     /gene="pilO2"
                     /locus_tag="ACHKA1_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFO5680588.1"
                     /translation="MSENNQNNRNFTSVIKNKRAFFSGLDWKTLPSEEKNARTFARKN
                     DAEYFLSCQYQDSENETKTMVAFIRKEDLPTGASSFWSLALMIKPLIEPDGYAICELG
                     DLYGFVSCVNNVLVNDVVGNKSQIMSALTTFLEFNETPEPGWKLYQPESWDISQALPS
                     LTLSALIDVKKPPKEAAFTRVSRKRQFMIYGGSAILAILLWNGITMYQEYREKEAAAE
                     AARLRLAKEMADKQAIQIAPPWQHLPEIKPFIDKCIDKWDALPLSIAGWRFDLAECST
                     SGNDGLLRTSYKELSGVTVEDFSTRIREIFQGTTTATFVLPEGSAGGFSLPVSFDVSP
                     DPITPDTLPQATDIQERLTTFAQKMRLKLTWQEIENTKTDEEGRPIILPWNEYELMIQ
                     TSTPPSILFANFHEPAVRFQYAGIKLEEGRLNYEIKGAFYVKNN"
     gene            23809..24303
                     /gene="pilP"
                     /locus_tag="ACHKA1_002969"
     CDS             23809..24303
                     /gene="pilP"
                     /locus_tag="ACHKA1_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO5680589.1"
                     /translation="MLKIIKPTIISVCFFSSLAYATESESTPKDEIKYSSPTILQVEQ
                     IQAETVIYEAKLARQKAVSELRNLDLNGVASATPAITPSSGYSSLADTDIPAQRVAAK
                     SLRVIEIFGTPQQMSARISLMDGSVTDVKVGQSIPGTSYKVASITGNGVWIQNGTEKR
                     QLIL"
     gene            24328..25866
                     /locus_tag="ACHKA1_002970"
     CDS             24328..25866
                     /locus_tag="ACHKA1_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO5680590.1"
                     /translation="MFFSDADYPGKDISDVVYVSISEKDDKPVHVYIADNSKGQRAVQ
                     AYIGTLNVKYPGKVNITWTTLDVIASRYQDSERRSDSNSSQHLNSNQEKVISYLAKAN
                     NLGSSDLHITPGRDGSEFTYVEARVHGELEILDVIPRKEGLELLGAAYSGMSDVIKGT
                     QFDPAIPQDARIAENFLKPVNLFGARYSHYPCVGGVYAVFRLIKDDSEDIPTFEELGY
                     MPQQIQTIRRMLQRPEGIIVLSGPTGSGKSTTLRTASEAYLSTFGFNHNDNMRLPRKR
                     LFTIESPPEGRIPGAIQTAVRDSVDGWVDAIKSAMRLDPDAILNGEIRDHASALAAIK
                     ASMTGHLLLTTLHANDALNIIERLEMENIQARLIADPLLFIGLLSQRLVQKLCPYCKR
                     TYAEMADKLSTEERKLIEENCLPEQVRLRNLDGCEHCYKGVTGRTVIAEVVSPDARFF
                     QLYRQSGRLEAKSYWHYELGGITRNQHLLHLINHGLVDPLSAHFISPIDEDKYSMLPE
                     GTWR"
     gene            25857..26966
                     /locus_tag="ACHKA1_002971"
     CDS             25857..26966
                     /locus_tag="ACHKA1_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO5680591.1"
                     /translation="MAIKDIFEKLNSIEIDFSGISHWLAKYTFRYSRISLYEDLSGML
                     NDGINVKRALTHLIDVETDYGKKTGSSATYYICCECLNALNNTGAISSALKKYIKEDE
                     YQLISSGEIRGDLAGGLVQAIKIVRSRSEMTMTLVMSMIYPSVLVFGCLANMYMVHDT
                     ITPVFLSLAPKERWTSSMRLLTDTSGFLIENGTYILCFCIVLFFLIRYSLARYTGPGR
                     QYLDYIPPWSLYKTFNGVSFLFNMASMLSIDIPVAKALERLEKNSTQNRWLLERIKEI
                     RRYVLLGQSLAMAMKSCGYDFPSKLCINKLLLHSENADNALMMSNYADKWLEEAKGKV
                     KSTGVWITVVSALIVFAFIVMMITALYDVSNVLQH"
     gene            27011..27568
                     /locus_tag="ACHKA1_002972"
     CDS             27011..27568
                     /locus_tag="ACHKA1_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO5680592.1"
                     /translation="MSSINILNMRSVFSSLSARRKKEQDKGATLMEVLLVVGVIVVLA
                     ASAYKLYSMVQSNIQSSNEQNNVLTVIANMKSLKFQGRYTDSNYIKTLYAQGLLPSDM
                     IADTTGASAKNPWGGSVTITTSSDKYSFNVVEANVPQKNCMAMVNALRSSSAISKINN
                     TSTSTVSAATVCASDSNTLTFSTDS"
     gene            27635..28117
                     /locus_tag="ACHKA1_002973"
     CDS             27635..28117
                     /locus_tag="ACHKA1_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO5680593.1"
                     /translation="MRSVLYILLSCISSLIFSLQPAYSATTTRFDACFAQAGQRYLID
                     PLLLKSIATVESSLIPDRINHNKEKGKILSTDYGLMQINSSHIPNLKKLGVIKDKSEL
                     IDNPCLNIQIGAWILATHFQKCGINWSCLGSYNAGFKESNEQKRIKYARYVYNKYMVR
                     "
     gene            28121..28756
                     /locus_tag="ACHKA1_002974"
     CDS             28121..28756
                     /locus_tag="ACHKA1_002974"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO5680594.1"
                     /translation="MLNSIFNTAILCCANIVCQCYAYNEVKFRLNKLNVSYKTGTEKY
                     YCLILTTLAVLYLSLNQLSLIQSIIVIVFYAFLTLMACIDLLSFLLPRLYTVTFIFSG
                     LLYQTWNNNILSGLFCAMLMFFIMLFVRLYFAYKNGTESFGMGDVLLIAGTGVWFPTP
                     EIACSIVFIAVIGGIIFFTLGGLNKQKKYIPFGPFLCGGMFVYSLVPGILF"
     gene            28769..>29788
                     /gene="pilV"
                     /locus_tag="ACHKA1_002975"
     CDS             28769..>29788
                     /gene="pilV"
                     /locus_tag="ACHKA1_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFO5680595.1"
                     /translation="MKKTDKGVSLLEVLLVIGIMVMVIPKVYENIENHLNNVRWQNAA
                     EHANTYNTAVRNYVADNASTLLAGSLPKTITPATLIQKGYLKSGFSESNFGQSYITGI
                     AKNSKTSRLEALTCSNGGQSLSEAGMRSVASMIEGLGGYINSSKQAIGAGGGWSDTPS
                     NYGLNCATGHIAMALVGADLQESDRLYRYSITNRPDLNRMHTAIDMNSNNLNNVGTLN
                     GNAAALSGDISARNGTFSGAISGNTATTNGDITSNNGWLVTKNSKGWMNSTYGGGWYM
                     SDSSWLRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLEKTATAGASCSPNGLVGRD
                     STGAIL"
BASE COUNT         9262 a         6399 c         6170 g         7957 t
ORIGIN      
        1 aacagtatta tctagatttt tagatcgtac atcatttaac aaacagaata ttattctgtt
       61 ttttagatgt tatccagatc attagatcgc tcttgtggca ttgacaggtt ttaaatgtcg
      121 cacacacaat actatccaga tgattgtaaa aagctcgttc atcgcgtttt ttgtgctcat
      181 tgcctggaaa atgagcactc tttgacgccc gttagaacgc ttgtcatctc atgctcttta
      241 acaaccccgg acagcggtca ttgcctaaag ccctggcata aggaggttat tattcgtgca
      301 tttcaagcac cgctggcatc tgtcagcaac gggctacgta acagagtgac gtgccgcgcc
      361 atctacacag gtaacgactc tttatccgca aatatcattc tgcatactta aaccccctta
      421 tttggtgaac tcgcgttgag ttaatctcac tcactctctc cctgagcaag tcgtacaggc
      481 tgcaacgcag aagtacacca gatttgccag tctccactcc aggtaagggg gtttagctat
      541 gcatgatgat gaacaatcag ataaagagcc tgtagtgagt ggctgcaact tttcataatt
      601 agcttatgac gctgtattcc ggggatgttt ctatttcaga caaacaaccg ggagaaaata
      661 tggcgcgtaa gttaaaaatt aatcctgtca ttcctgaatg tgtggcagat ttgaaaggat
      721 atccactcta tatcatcgtg gcttattggg gattaagaaa taataaaacg ctcacaacaa
      781 cggatgttag ccgtaatttc cttattacac agcaacaatc ttatgatgtg cttacctaca
      841 tttaccagga agcacagaag cagataacag cgactaaacg aacgctattt aacgagcaca
      901 aaagaagaat tagtgcgttc aggattcttg atattgacaa aggcatattg cgcaccccaa
      961 aaaaagagat tgcatatgcc agctacgaca atataccaac tgtcacaatg aaaagtaccc
     1021 gttgtgagat ccaggatagt tttacagaac tgcgccgctg gatgtgttca agaaagacag
     1081 gtgatcgagt ggaggtattt ttgaatgcct aagtaccaaa taaacgcaac catcaaaaaa
     1141 ccaggtggtg gccccgtgaa atggactcat tacagcgact tacctatgac cgctgaagaa
     1201 tgcagaaaaa acttctcaaa agaaaaagaa gcaggaaagt gtttttctgt tgatgttcat
     1261 atttcagatt tcatttgtaa agaaatacat ccacagtgat gaatggggag aggattaatc
     1321 atgattgacg ctataattac gccaatagtt acatctattc cgttactctt attaatttta
     1381 gtcgcgctga tattcgttaa atctgccgtc aaaatcgtgc cgcaaggtaa tgcctggaca
     1441 gtggaacgct ttggtaaata tacccatacg ctttcccccg gtcttcactt cctgatcccg
     1501 tttatggacc gcataggtca acggatcaac atgatggaaa ccgtcctgga tgttcctaaa
     1561 caggaagtta tttcgaaaga caatgcaaac gtaactatcg atgctgtttg ttttatccag
     1621 gtcattgatg cagcgaaggc agcttatgaa gtcgataatc ttgcatcagc tatttcaaat
     1681 cttgtcatga ccaatatccg aactgtcgtt ggtggaatga atctcgacga tatgctttct
     1741 caacgtgaca gcatcaattc taaactgtta acggttgtcg attatgcaac tgatccgtgg
     1801 ggaattaagg tcacacgtat tgaaattcgg gatgttaaac caccagaaga actgactaag
     1861 gcgatgaacg cgcaaatgaa agcggaacgt actaagcgtg cccaaattct ggaagcggaa
     1921 ggtatacgtc agtcacaaat tctgaaggcg gaaggggaaa aacagtctca aatactgaaa
     1981 gcggaagggg aacggcaatc agcatttctt cagtctgaag cacgcgaacg tcaggcagaa
     2041 gcggaagctc gcgcaacaaa actggtgtcg gatgcaatcg ctgaaggtga tgttcagtct
     2101 gttaattact ttattgcgca gaaatacact gaagcactcc aggcaattgg cacagcatca
     2161 aacagtaaac tggttatgat gccgcttgat tcaagtagcc tggtgagttc ggttgcaggt
     2221 atcagtgaac tgctaaaaaa cgtatcacag gatactccca aagttgatat gaaacaggct
     2281 caacagtatt tgtcttaaac agataccgga cggtgacaat attgttttaa tcaccgtcct
     2341 tatctcatgg agattaacga tgttctggtt tacgctgttt cttctttgta ttgcccttga
     2401 aattataacc ggaacgggat ggttactcgt aatcagtctt ggtgcattat caagtgccat
     2461 tgttgacttt ttccttccta tatcacaaga agcaaatata tgcttctttg cgtcaataag
     2521 catactgacg tcgataatta agttcgtcta tgacaaaaaa cataagaaat tagatacacc
     2581 cctggtgaac acaggacata cacgttttaa aggaaaagaa tttactctca gtgacgatat
     2641 tgtgaacggc aaggggcaat tacttattgg tgatacattc tggcctgttg agacgttaca
     2701 cgatgaaaac taccctgcgg gaacaagagt tattgttaca gacatgcaag gaataactct
     2761 taaagtaatg accatcgaag acaagagtga aaacaaggaa taaatcatgc atctttcgtt
     2821 gttaattccg cttacaggag cagcgatgaa tgcactatgt atttttatta tcagacttct
     2881 ggataaggat tttattgaag tctataaaaa aacgctaaaa aaaattactg cattacatat
     2941 tttattgatg gtgttaagtg tcacaggaat agtatgagga caattctatg cgaaacgcaa
     3001 atgaaggaat ttctcttgca gctattgtag ctaaagaaac aacaacaggt aaatggaatg
     3061 gtactggtgg gggtattggt ttaggtactg gtggtattgg cttattcatc ggaggtgttt
     3121 ctggttctaa acaagagcag acacaaaggg caaaaaactt tgaatcgcct aaagaggaag
     3181 aatacagtct attaaatgta tacggttcga taataaaaat tctcgttttt ggcgtgctat
     3241 ttggtttatc ttcccgttta tttacatttt tatcagacac tggttctgat tttgctgaac
     3301 cagtaacaac caccacagcc acaggaaaga ttcaatctat tctgggtggc atggcagaaa
     3361 tggtaagcat tttaggcacc gttgtccctt ttttagtaat tatcatagca atgatctggt
     3421 ttctgttttt cagcagcaaa cgagaaaaag aagagttaac tcgcgttgaa catgaagctg
     3481 ctaaaacaaa actccgtata gatgtctatc accgtttacg ttacgttgaa agcgatcatg
     3541 tcgtttttga cccgattaca ggccgggagg ttccggcaga acgggcgtgt attaataaac
     3601 tgattgaggc acttgttgat gaaagcaacg acatctcaaa aagaaattga cgaacttctg
     3661 aatgcaataa aaacaggaca ttcaaaattt attctatcaa tacccgtaca tactacccat
     3721 ccaatggccc ataaaataat gaagccttgc ttcaggagaa caggaccgct tctgtattca
     3781 gatagtatga ggaaaattca atgcgaaacg caaatgaagg tgtttctcta acagaggcta
     3841 tattagccat tagaggtata gataaatacg cgtcatacaa aaccataaaa acaataaaaa
     3901 ccataataat aactatagtt cttattatcc ttgcattagc tttttttagc cttgtatcaa
     3961 gccttgtatc agcagcagga agagttgctg tagctataca ggcagtatgc tacctgtgcc
     4021 tctgtatggc aattatcatc atggttgtca tcaaaataag ttctttcatc gacgaaaggc
     4081 gagaaaaatt agaattaaaa cgtgcagagt tggataaaat taagataaaa gctcgtcaaa
     4141 aaatctacaa acgcctgcgt tacgttgaaa atgaccatat cgtttttgat ccggttacag
     4201 gccgggaagt tccggcaaaa tgggcgtgta ttcataaagt cattgatgaa attatcgccg
     4261 ataaaagcaa caacgtgatc atgttcgacc cggttactgg ccgggaattt ctcgccgatg
     4321 gatgcaacaa tgtgaagaag gtagcagccc ccctgaagga tacgatgaaa tgtacagaag
     4381 aaaacatcac ctaataaaaa tatttttgtt aagaatggcg tgcaatgttt tttcttttct
     4441 gctgggtgct ttcacactct cggcaccatt tgtcttttat acattaatcg gttcagttct
     4501 taatgccgtt attcatttca tcttttcctt gcctttagcc gccatattcc tggcttatgc
     4561 gtattcaggt tttaaagaaa gcatttatct attcatggca tataaatatg actcaagcaa
     4621 aagctaatct gagaaaactt cgccgtgcac gttatgttcc ccctcgaaca gtaaagaaaa
     4681 gcataacgaa tacagaacgt gtcgtttcaa aagcaattac cggctgtggt gtcttccgtg
     4741 atataccaca agtaaaaatc aatctcatcg aaacaaggaa aattttacaa atcgaactgg
     4801 ctttatccat tgctaactta tctgaggcga atccaattga gatacgaaaa aaaatcaaaa
     4861 gcattgaccg acaattatct tcaacctcaa aaatcagaac tacattatga aaaaaaacga
     4921 cgatttaatc actttaccat ctcataacag ccacgctctc gattacatca ttaaaaaagc
     4981 gtttacacct cgcaaaacca aaacatcgac agcgacccac aacgtttata gttacaataa
     5041 agaagaatcc acattaaatt tccaaaaaat tggagaagca atttttttcg gcacagacct
     5101 ggtaattaag ctgaagccaa atattagtgc ctctggtgaa ctcttcatct gccagaagga
     5161 ctaagccatg tacattgttt ctaaaactct tgagctagta acaaccattg ttgaatccac
     5221 agcggcgacg aacgccgcta aagtcgcaca agcgatatct cctacattct ttgctgccat
     5281 aaccttatac gttatttatc taatatatga aatcacgtat gcccagcgtg atgtcctaat
     5341 gaacgaggta actaaaaata tcggcgcatt cgccctggtt ggcgcgttca cttattcagc
     5401 gccttattac agccagtttg ttattccttt tgtcatgcac gcaggttccg acctttctgc
     5461 tgctgttact ggtggttcag gaactgcaac cactgttgat aacttatggg atactctatc
     5521 tacgagttta aatgacttta agaagacaaa tcttgaaatt cttgcatgga ctgacatcac
     5581 agagaaatta tatatatatc taatttgggg ggttggatat gttggggggc ttttacttat
     5641 ttattacacc gctgtatttc tagcgctatc aacattcatg gttggcattc tcttatccgc
     5701 cggaatatta tttatctgct tttctctatt tgcttcaaca agaaatatgt ttacagcatg
     5761 ggtggggagt tgtcttaact acattctact taatcttttt tacagcatat ctttcagttt
     5821 cgttatcggt tttattgagc aaaccgtccc gtcaagtgga aatataaccc tcacaacagt
     5881 ggttaatttc tttatgatca ttgttatatc tatattcctt gttgagcaag taggtactct
     5941 ctgctcaact cttactggcg gggtaggtat aaatggcctt acatcagccg ctaatggctt
     6001 tgggggtaag gttgcatctg gttttatgcg agcatcagga ctccgatctt tcactaatgg
     6061 atttgccagc aaaatgccaa atccaatctt taatgcagga agtcgttctg catcagcatt
     6121 agttcaggga ttaaaccgtg gaagtaaact taaagcgggg tgattaacat gaaagataaa
     6181 gtattatcat ttctctttgc tggaataatt accgtaacat caccatcaat ctttgcatct
     6241 ggtattccag tagtagacgt tacagcgata gctaaaacag ttgaagaagg gctaaatcgt
     6301 gccgctgaag cggcaagaca gcttgagcaa ctcaagcaac aatatgaaca gaccataaga
     6361 tacgctgaag aacagaagaa aagactcgaa ggcttcactg atttcagtaa cggctttgat
     6421 agcgcatcca gctatatgaa ggattctcta tcaacaataa caaatagtgc aaaatcagat
     6481 cttagctctt tgcgatcgca atatgactta tcaagtaacg ttgcagaaac gcaaaaaaaa
     6541 tatgatgcaa tcctggctaa gatcaaattc tatgaaaact tcaacaatga aatgcaggaa
     6601 agggctaaga gattaaccac gttacagaag gagttcgcgt cagcagatac tccgcaaaaa
     6661 aaagcagact tatctaacca attgaataca gaaaaattga caatggagct acaattgaag
     6721 caatatgata ttgcggagag acagcttgaa gctgagcaaa aagcccaaca tcaagctata
     6781 gtaaatcaat tgatggatag gttaacttaa agatgattaa cttacgcagc gcaattatcc
     6841 ctctattaat ggtctcaaca acattaatac tttctgggtg taaagaaaaa gtttacgctg
     6901 ttgaatacta ttcaagcaac ccagccgaag cagctaaaac catcgaacag tgcagaaaag
     6961 gcgaaatcac agaccagaat tgcgataatg ctcgtgctgc attagaacaa gctcaaaaag
     7021 aagagcacaa gaagaaagtg cgggatctaa tggaacgatt agattaatat gagtaagcga
     7081 aataataata aaacccgctt cgtgcgggtt ttatcttgct taaagatcta tgaaacctcc
     7141 ggcttcgggt attaaactat aacttgcttt ggcaaatatt gtttcattga cttcatggaa
     7201 tcttattttc tccgcctgtt catatgttac agggaagaac acgccccctg gctcattaaa
     7261 catcataaaa tcaccttgca ttgcgttaca atgacaacaa tgattcgcgt aatagttcat
     7321 ttttgctgtt ttgctatggg atggataata taattttgaa tactcattaa tccttgagag
     7381 agcagaggtt gaaattattt caattttcgt taaatcagtc caccatactc tactggttaa
     7441 ccaccgataa atcatatttt tctttgaatc aaaatactcc atacccttag gggaggatgt
     7501 ttcataccat tcatctaata tacaatcttc ccattcggca ggatcaactc cccaaataac
     7561 gtctctatac ttataccaac gaggtaaaaa aataccaaac acaggagttg ttttcttaca
     7621 acgccaacaa gactggtaac tttccgcaat ataaaaacgt tgcgaaaaaa cattgtaatc
     7681 atgttcattt aattctttta tccaccgaat aaaataaatt ggatttttcc cctgtggaac
     7741 aaaccatttc cgttgttctt ggtcccattg cgcccctttt tgtttagcca aattcttttc
     7801 ttcaaacgga acatttaaat aaaaccgcgc catagccaac ttaactccag tattctgttc
     7861 tgatgatgca ccaggataga tatcaaaact aaacaaagag tgcaattccg cctttaatgg
     7921 ctcattcata cccgcctcgt acgggttttc ttttgcctga aatatggagg aactccatga
     7981 aatccatcat tttatcgatg gttgccgcca ccgcattact gtcaggctgt gcggatagca
     8041 aaacacaacc agtacctgag tcaccgttaa ccaaacatga ccaccagata acgttgaaaa
     8101 ttgataccgt gcgaacagac ctgccggaca gttctcctgt ttgtttgaat ggtatgaata
     8161 tatacgaaag tctgagcgag cggcatccag gcttgtttgc caaaaatcag aacgtgattt
     8221 tccgtagtgc attctttgat gacggtcagg cgatatcctc cgaaacaatc cgcaaaataa
     8281 tgcagagtac cccgtgcgtt gaatcacaaa caacatctgc cggaagctgg gaacctgcca
     8341 cggaactgat cccccgtcag gcaatatccg gtgacactac actggctgaa aaagatcgga
     8401 ctgctggctt cacaatgcgg atagcgccgg agattgagcg tggaaatttc cacctgcaac
     8461 tcacaaccac tgacccgata cggaaacaga ctgtcgagca gaatatttta ctcaaaccgg
     8521 accagcccct tatcgtgagt ggttttactg gagttaatgc tgatagccaa agtgagaacc
     8581 ggattgtcat cattacgcca cacgtgcgtt gattcccctg atatttaaat tacccgcctc
     8641 gtgcgggttt tcttttgtct gaaatatgga ggaacaccat gaaatccacc attttattga
     8701 tggttgccgc caccgcagca ttacgaccag gctgcgcaga tagtagaaca caccccgtgc
     8761 ctgagacacc gttaagcaaa atcatgttat cgatgattgc cgcaagtgct atcgcactct
     8821 ctgcccccgt atatgcgtct gatccgtgtg cttccgttct ttgtctgtac ggtaaagcta
     8881 tcggtagcgg cggcgggagc gaatgcaaaa gcgccgaaaa ggacttcttt aatatcatca
     8941 aaaagaaaaa gggatctatt cgctggagca aaacctttga tgcccgaaaa gcgtttctta
     9001 atcaatgcac gaccgcagac cctgcggcga tctcaaaaat catgagtaag ttcggacgtt
     9061 cctggggtta accatgaaaa agctcattat tattcccacc acattattgc tgaatgcgtg
     9121 ttcccatgcc cctgaacccg cacaggcaaa aggaggttgg tatgaactga ataccacaat
     9181 tgagcaggta agagcagaca catactataa cggggtgatc cttcagcagc ccgtgtcaca
     9241 gccaaacagc actgttcaat acagcaatga taattatctc aaaaaggaag ccataaacgt
     9301 aactcccgcg cctcgtattg cagaaaccgt aaaaccacgt tctgagcaat cagcgacagc
     9361 gccaaaaaac cagggcagca ataatagttt tctgaagccg acaacaaaca gcataacttc
     9421 atctggtgag ataaaaacag cagccaaagg agcaactgaa cagcattctg aacaaccagc
     9481 actccctccc gcaaagccaa tcagtagcaa caaccatcag gcacaaacgg cacaaaatac
     9541 tgatcagcag acaaaaccgc gtcctgaaca accaccgcta atgtcaaaaa gcggttttct
     9601 gaaaaaaaac gctaacaacg ctgttccggc caccacgctt acaggcccat caacacctga
     9661 aaagcaaatt tactttgttc gccatgatgg taaaggctcg ctgcaaaaag cagtaaaaga
     9721 aattgtccca tcggactgga cggctgaaat atccccggag gtaacgaaaa ctttccgtcg
     9781 cacgatatcc tggcagggca acgatcaatg gccttatgtc ctggataaaa tgcttcgcaa
     9841 ctacggtctg acggtaatta agaacaccga aaaaagacat cttctgattg attttcaaca
     9901 acagaaaggg aaaacatcac aaaaagcacc tgtatctcct gacaaaaccc ctgcatctcc
     9961 tgtcaggaca ccagtttcct ctgacaagtt acctcaaaaa ccgctaacgc ctgtcaccgt
    10021 tccgcaaaaa cagacagaaa aactcacctg gaatcttaaa aaaggttcga cgctgcggga
    10081 tggcctcaca gaatgggcgt caagttcgcc gtgtggtaat tcaacctgga atcttgtatg
    10141 ggatactcct gtgaactaca gaatagatgc tcctcttatt ttccggggaa actttgaatc
    10201 tgcattaaaa gatacgctga tgctttatct ttcagcagat aaaccgttat acgcattcag
    10261 gaatattgca caatgcgtaa tcacaattaa agacacacaa caggagtaat cgctatgggt
    10321 tggttcttca gcttgctcct gatattcctg tcaataggca gttactatct gacagataac
    10381 aataaccaaa ccatacagcg aatgaagttg cttcctgcaa aacaggaagc aattgaattt
    10441 gtccatttcg caaatgttat caatgactat ctttataaat atccagacaa acgtaattca
    10501 ggaggaacgt taacatctga gcaaatcggt ataacacccg tttatgatat tcatcatatt
    10561 atttacggta agcgagttta tatctggtct gccgatacag aaggtctgat gtctgctttg
    10621 cagcagcaaa caaaacattc agcaatgtta ggaagagtaa aaaataagaa gattgtcgat
    10681 aatcagggga atgatatggg cgttcccgtt ccttcctcaa ttccagaaga ggctattgta
    10741 ctcattaact aaatatcccc tgacaagggg attctgaact ggagtctgca tatccgacac
    10801 atcattatgt gggccggata tatcgctacg gcgcggactt cagtccagaa aatctttacc
    10861 ggagataccc atgcaggata atgatgatgt cagcgagctt gatattgcca ctcgtgaagt
    10921 ccttatctta cgatgcaatc ttattcagct tgcttctgaa tatataaaaa taaatcccct
    10981 taaaaacacc agaatctaca cagatttaat tcatcgttca gcagatgtta tcaaaaatat
    11041 cgaacaaggt aacatccaga ataaacataa ttatctgtca caggatgtaa aacatacgct
    11101 tgttttatac gacctgtaca aatcagactt tgaacaatgt ccgggggcaa aatcccttat
    11161 acctgtcagg gaaaaaattg tctcgctgaa agaacttctt tccggcgcta atgagctgga
    11221 cagtatcgtt cgtcgccccc gcaggaaaaa acgttcttca cgaatttaat atcaggagat
    11281 aaatccctta tgcagttatc agcggctgtt ctggcagcac tgataagtca gtgtgcccct
    11341 gatgtatcac ccgatacgat gaatgccctg attatgactg aatcaggggc taatccatat
    11401 gtaatagcga atgtttctga cggaacaagt aagtatttta aagatgaaaa aggagctatt
    11461 gaatatgcag aaaaattaac agcagaaaat aaaaggttta gtgcaggtct gacacagata
    11521 tacagcaaaa atttcccatc actcaatctg actaataaaa cagtttttga cccgtgtaca
    11581 aatataaaag caggagcggc agtcctgacg gacaattatc tgagacaaaa ggagggtagt
    11641 agtaatcaaa aaattctccg cgctttatct ttgtattatt cgggtaacga atcaacagga
    11701 tttattaaag aaaagaaatt taacaacact tcttatctgg aacgggttat tcgtaacgcg
    11761 aataactata tcgtgccatc aatccgtgaa aaaactgatg aatcagaaat aaaacccccg
    11821 aatgatgaag agaaaacatc accggactgg gatgtttttg gagattttag ttaatgaaag
    11881 tcaaatcaac tttgcagtat tttatcctgg cgtttttcct gtcactaacc gcctccgttg
    11941 cctatgcagg tggactcgac accgcaacaa ataccatgac agaacttaag acatgggcat
    12001 ttggctttgg tggtgtttgt gctttgtgtt acctgatcta taacatcatc atggcattca
    12061 tggagaagaa aagctggtct gatgttggaa tggcgcttat ctattgttcc ctcgctggcg
    12121 gtgcacttta cgccgggaac tgggcgctgg aactcttcaa ataagtaatc catatataca
    12181 actgtgccgc atatttacct tatgtgtgaa ttgcggcttt tttcatggtg agttatggac
    12241 aaagtaaatg aacatgtcag tgagtcattc atgacttata acgggtttaa ccgtcctgcg
    12301 ttagtttctg gtataccctt aatgttgtta ttgttcacgg cttttttcgc tgttctgaca
    12361 ggcttccccg ctattttcct ttggggtata aaagggataa ttattcctgt catatgtgcg
    12421 ctgtttcttt ttatagtaaa actggcctgt gaaaatgact caaacgcact aagagtcatc
    12481 cgtttaaacc ttatggggct tttgttaaaa atccgccaca gagatttaat tattggttat
    12541 tcatctgtca ggtaacatat atgcacaaaa tcaaattaaa aaacagtgac aatatggatg
    12601 ttgctcgtga atatcccgat tatcgcttcc atataacaga cagtattatc tttacttctg
    12661 acaggaaaat gctggcttca cttgttgtag caggcattcc atttgagaca gagaacgata
    12721 atgttcttac aaatttattt aacagcgtta aaaacttcct gattgggtta ggaaaggaag
    12781 gcgacctgta tttatggact catcttatca aaaaaagaat aaccattgat ggagaatgga
    12841 attttgacaa tgctttttta agcaggtttt caaaaaaata ccttgctctg ttcacatcca
    12901 gcgcattcta caaatcaaca tggtatttaa catttgggat tccctacgat gatgttgata
    12961 ccggaatcga gcgcatgaat gaaatgacac aacaggcgca aaaagcactt cagccattta
    13021 acgcttctgt tttatcagtt cacaatactt atattagtga ggtggcagac tatttatcac
    13081 tattgctaaa tacagaacat aacatgattc ctttatcatc cactccctta tcatccagta
    13141 tttgtgattc tgaatggtat tttggtgctg atgttcttga actgcgtaac aacgaatcgg
    13201 atagtaaaaa gtttgcaacg aattatattt tgaaggattt ccctatagaa acgacccccg
    13261 gacaatggga ttttcttctc aagcaaccct atgaatttat tcttactcaa tcgtttattt
    13321 ttgaatcacc cacaaaaacc ctgaagaaca ttgattcgca attaaacaaa ctgcaatcag
    13381 ctaatgatgc agccaaaaca caacaggaag aactcgaagc tggtaaagaa gcggtcgcag
    13441 ccggaattac gttgtttggt tcactacact gcgcattaac ggtattcggt gatgcaccag
    13501 atcaggccag aagcaatggt ataaagctct ctgctgagtt tattacttcg ggtaagggat
    13561 tccgcttcag tcgcgcctca ctcgcatcac cttttgtttt tttcagtcat atgccgctga
    13621 ataagaggcg tccactggat acccgaagaa caataaccaa cctggcctgt ctgatgtctt
    13681 ttcacaatta ttcctccggg aaaaaatcag gcaatcctat tggtgatggg tccgccataa
    13741 tgccgttgaa aacggtgtct gacagcattt actggttcaa tacgcattac agcccaccgg
    13801 aaaaaaatgt taccggacaa aaaattgccg ggcatggaat gattcttggc gcaacaggca
    13861 cagggaaaac aacatttgaa gcagcagcca gcggttttct tcaacgcttt gaccctttaa
    13921 tgtttgttgt ggatttcaac cgttctactg agctttttgt ccgtgcatac ggaggaagct
    13981 atttcacgtt gcaggaaggc atttataccg gctgcaatcc gtggcaactg gcagaaggcc
    14041 cggaatctcc cgtctggcac agacttctcg catttctgaa acgctggaca caggttctgg
    14101 cacgagacaa ccagggcaac ccctgttctg atgaacatgg cattgaatta aacgctgccg
    14161 tggaaagcat tatgcgaatg cctgtcgaag aacgccggac agcccttctt ctcgatatag
    14221 tcagtcctga acttcagacg cgtctcgcca aatggtgcga caacggcgaa tatgcctggg
    14281 cggtggattc cccccggaat accttcaatc cactgcatca taaaaaagtg ggatttgata
    14341 caaccgttgt cctggacaca aaaaacggta ttcatcctgc ctgtgaacct ttactcgcgg
    14401 tcctgttttt ctacaaagaa atcatgcaac gcggcggcaa tctgatgctc tccattattg
    14461 aagagttctg gatgccagca aactttccca tgacacaaag catgattaaa agcgcactga
    14521 aagcggggcg catgaaaggt gaaatgatgt ggttaacatc acagtccccg gaagacgcca
    14581 taaactgcgc aatttttgcg gcgctggtac aacaaaccgc aacgaaaatt ctcctgccca
    14641 atcccgatgc aaaatgggaa ggatataaag aaattgggct tactgaaaaa gagtttgaaa
    14701 aactcaaaga gctgacgaag gagtcaagga ccatgctcat caagcaatct ggctcttctg
    14761 ttttcgccaa aatggatttg tttggattcg atgagtttat ccccgtgctt tctggctctg
    14821 aaactggcct gtctattttt gatgagatta ttgctgaaaa aggagatgta gctcctgata
    14881 tctggatacc agaactcctg aaacgcctga acggttaatt tctctgtttt atacagaacg
    14941 gaaaacaata tgaaaatccc cctcataatt gcctccgcgc tattaattag tgcatgttct
    15001 cacaccccca atcctgtgca gaaaaaaggg ggatggtttg agttaaacac agcaattgaa
    15061 cagatcaaag caggtgatta ttaatggaaa acacaaacta ttatgaacat tcaaaaaaag
    15121 aagccaccaa aaagaaattt gaagaaaaaa acgaaaaaaa ggattatttc aaggccatac
    15181 gtgactttga gcgcagtgaa attgaaatca taaagaaaaa ggcaaaaaca tttaccattc
    15241 tcgccattgg tgaatttgtt gtaatttgca ttcttggttt cgcaatagcc tcccttgctc
    15301 cattaaaaac cgctgtaccg ttccttgtgc gagtggataa cagcactgga tatactgata
    15361 ttgcccctca attatctgat gctaaggaaa gttatcagga tgttgagact aaatatttcc
    15421 tgtcaaaata tctcattaac tatgaagcat acgactggca gaccatccag gaacaggccg
    15481 atacagtaaa aacaatgtct tcccaaaagg tgttttcagc ttatgacacc atgattcgtg
    15541 ctgattcctc accactgaac attctgaaaa acaactacaa aattaaagtc cagattaatt
    15601 cagtaatcct tctaaggaag gatatggcgc aggtgcgttt caaaaaaatg gtactggatt
    15661 tatctggtaa acccgccccc ggatacagag caacagagtg gatttcgacg atttcttttg
    15721 actgggataa agatatcaaa acagaaaaag agcgccttgt gaatccgctg ggactgcaag
    15781 ttctgagcta ccaacctgat ccagaggtta taaaatgatg cttaaacgca ctactgcgat
    15841 agttcttttg tcgatgctac aagtacctgg tgctttagca gctatgtacg gcacaccatc
    15901 ggaacgagac gggcgtattc agaccgtaga ttataacgag caggatgtat ttaatgtgcg
    15961 cgtaaaagca ggcgcacaaa caacaattaa gttcggacaa gacgaaacaa taaaagatgt
    16021 cggaattggt gatccagaag cctggtcagt atcggtcagg gacaacactc tgtttctgcg
    16081 tccaaaagca gaagaaccgg atacaaacgt taccgttcag accaataagc atatatatcc
    16141 tctttacctg atatcaacca cgaaacaacc cacgtatatt ctgcgtttta attatccgaa
    16201 accaccgtca gctaccgtaa tgactgaaaa gccatttcca tgcactgatg gtggaataat
    16261 taatggtcat tatcaactca agggggataa atcaattttc ccttatcagg tatgggataa
    16321 tggcgaattt acatgcatga gatggactaa taaacaggaa attccggtcc tttatcgtgt
    16381 tgatgccgat ggaaatgaac atctggttaa cggagacaga aataaaaaca caatggttta
    16441 ctatgacgtg gctgaaaatc tccgtttgcg cctgggtgat caggttgcag atatcagaac
    16501 atcatcaatc gttaatcgtc cgtggaataa aaaaggtacg tcaaacggaa aaacacgagt
    16561 agagaagttc agctatgaaa aataaagatg atgaaaaaaa taatgacgcg ggaaatcgcg
    16621 gaattatcga agtaaaaggt aaagcagcac caaaaaaaat attaatcctc atcatattac
    16681 tgattgccgc gttatttgtg atcattatct tgtttaaagt tctttcacga gaacaggtcg
    16741 ttcagcaaac acctcttgag aagtctgacg agacactggt cacaaatacc aacaatggcg
    16801 tttcactgac aacaatgatg aaaaacatcg aagaaaagga aaaactcgat gccgctaacc
    16861 gcaggaaagc acaggaggat caagaacaga aacaggcaga caatgccccg tcagcgccag
    16921 catcacaaaa ggccgatcaa acagccgtca atgtgatagc caacggcact ccccaaaccg
    16981 ccagtggaga tccaaaccaa ccacaaccat tgccaaaatc cgtccgacaa ctgatgggcg
    17041 acacaatggt gaagatcgac aatcaggaac caggcgaaaa aaaccaggaa agagatgatt
    17101 tacagggcag tcagtacgca gacggaaagg tatcccctgt gctgaaccgc cgatatctgt
    17161 tatctgcggg aacagcatta tcctgtgtgc tcaaaacgaa aatcgtaacc tcttatcccg
    17221 gtatcacaat gtgtcagtta acacgagacg tctggtcgga taacggcgaa gtgcttcttg
    17281 cccgtaaagg agcattactg atcggggagc agaataaagt catgacacag ggagtagccc
    17341 gtgtgttcgt taactggaca acgctcaaag atgaaaatgt caatgtgcgc attggcgcac
    17401 ttggcactga cagtctgggc gcttccggcc ttcctgcatg ggtcgataat cattttggtc
    17461 agcgttttgg tggtgcattg ctgctgtcat tacttggtga tggtttagat attctgaaaa
    17521 acagcacgca acaaacaggc tcaaacagca atatcaccta cgaaaacaca tctgacgcaa
    17581 caaaagaaat ggcaaaaaca acgctggaca atacgataaa tattcccccc acggcatata
    17641 ttaatcaggg gactgtattg tctgttattg ttccgcgaaa tatcgacttt tcatccgtat
    17701 atgaacttca gtaattcagt aattcagtaa agcaattgat atgaataaca tcagagatac
    17761 cagtaataca tcacaaccac gagataacag taaaaccgca agacgatatc tggatatgac
    17821 gggtatccag tctgttctta atattgaaca tgtcacagaa atatctgtga ataaaccagg
    17881 aacaatatgg tttgaaggga aaaatggctg ggaatctaaa gatgcgccag acgcgacttt
    17941 tgataattta atgacacttg ccaaaactct gacgaactta tcaaagataa aaataccact
    18001 tagccatgat aatcccattg catcggtagt tcttcccggt ggggaacgtg gacaaatcat
    18061 cattccacct gcaacagaaa ataacagcgt tgtgatatct atccgtaagc catcattaac
    18121 gcgctttact atagatgatt atgtccgaac agggcgtttt gataatgtca ggattgcgac
    18181 gaaacacgaa gccatcctga ctgaacggca acgctacctg tatgaattat cccggcgtcc
    18241 agatggtcaa tctaaagctc aatttttaag agaggcagtt aaagaccgtc ttaatttttt
    18301 aatcgtcggt gggactggct ccggtaagac aactatagca aaggcaattg cagatatctt
    18361 tccacctgaa cggcgttata taaccgttga agatgtaccg gaaatgtctc tgccgctgca
    18421 tccaaaccat atccggcttt tctacaaaaa aaataccgtt gaagcgaagg aaatcattga
    18481 agcgtgtatg cgattaaagc cagatcacat ctttcttgct gaacttaggg gtaacgaagc
    18541 atggagttat ctggaggcgc ttaatacagg gcatgaaggc tcaatcagta ccattcatgc
    18601 taataatact tatgcctcat tttcacgact ggcaagtatt gtaaaacaat ctgatgttgg
    18661 tatgactgtt gatatggacc tcatcatgcg cacgataaaa acatcaattg atgtcattct
    18721 ttttttcaac cataccagaa tgactgaact gtattacgaa cccgaagaaa aaaatcgctt
    18781 tttgagcaca atgtaaattc tggagataac tatggatgca aaaaagacgg gggggttaat
    18841 tctcttcctg ttactgcttc ttgtgggtgt gcttatagcc tcaaactatc tcggtggata
    18901 cacagcatta agatattcat ccgttgatat ttctttgctc aagtgggata cattccattc
    18961 tgtaatctca acattttctg gcaacccaca atataaaaaa ctcgttttta tggcatggtt
    19021 tggattcagc gttccattaa tattctttgc catattcatg ttaattgtcg tgattgggat
    19081 tatgcctaaa aaagtcatct acggtgacgc ccggctcgca actgatatgg acctgagtaa
    19141 atcaggtttt ttcccggata aaaaatcacc ttacaaacat ccaccaatac tcatcggtaa
    19201 aatgttcaag gggagataca aaaaacaatt tatttatttc gccggacaac aatttttaat
    19261 tctttatgcc ccgacacgtt ctggtaaagg ggtggggatt gttatcccca actgcgtaaa
    19321 ctatccagga agtatggtca tactggatat caaacttgaa aactggtttt tatcagccgg
    19381 attcaggcaa aaagaattag ggcaagaatg ctttctcttt gcgcccgccg gatacgcgga
    19441 aactatcgac caggcaatta aaggacagat acgctctcac cgctggaatc ctctggattg
    19501 cgtcagtcgc agtgatttac ttcgtgaaac ggacctggca aaaattgccg caatccttat
    19561 cccggcaagt gatgatccta tatggtctga ttctgcacga aacctttttg tcgggttagg
    19621 actttacctg ctggacaagg agcgttttca cctggaacag aaagccaaag gtcataatgt
    19681 accggacgtt cttgtttcca tcagcgccat actgaaaacg tcggttcccg atggcggcaa
    19741 ggatctcgcc gcatggatgg gacaggaaat tgaaaaccgt agctggatta gcgataaaac
    19801 aaaatcattc ttctttaagt tcatgtcagc accagatcgc acaaggggaa gtatagaaac
    19861 aaacttttca tcaccgctga gcatcttcag caaccctatt actgctgagg cgacgaattt
    19921 cagtgatttt gatatccggg atattcgtaa aaagcccatg tcaatttatc ttggtctgac
    19981 accggatgct ttaatcactc atgagaaaat tgtaaacctt ttcttctcat tactggttaa
    20041 tgaaaactgt cgtgaactac cggaacacaa cccggacctg aaataccagt gtcttattct
    20101 cctggatgaa tttacatcaa tgggtaaatc ggaggtcatt gaacgcgctg ttggttttac
    20161 agcaggttat aaccttcgct ttatgttcat tcttcagaat gaaggacaag ggcaaaaaag
    20221 cgatatgtac ggtcaggaag gatggaccac attcactgaa aacagtgctg ttgtgcttta
    20281 ctatccgccg aaatcaaaaa atgctctggc taagaagatt tcagaagaga ttggggtgcg
    20341 tgatatgaaa atatccaaac gctcaatttc cagtggtggt ggtaagggag gaagttcacg
    20401 tacacggaat gatgatgtaa tagagcgccc ggttcttctt cctgaagaaa ttgtgtctct
    20461 ccgggataag aagaataaag ccagaaatat tgcaatccgt gaaattataa ccagtgaatt
    20521 tagccgacca tttattgcca acaaaattat ctggtttgaa gaaccagagt ttaaacgccg
    20581 cgtggatatc gcccgtaaca atcatgttga cattccaaac ctgtttacac aagaagtcat
    20641 ggatgaaata gcaaaaattg cagaaattta tctccctaaa gctggaggaa aaaaagtcat
    20701 ggttgccgga ggcaatgtga ttactaatcc agaccttgat aatcacgata aaacagacgt
    20761 atctgaataa tcctcgcaac cagcaaatag catcttgcat attccataag gtaattaaac
    20821 atgaataaaa aattgctttg tttattaata tcctcttttt tattatcagg ctgtgcaaac
    20881 ttcaaaaaaa tcaatgctaa tgaacttgca gccgaaagag acgcacaaaa agcgactgaa
    20941 tacgcgaaaa aaattcgtaa taacccggtt atccaggact caacaaaaca atggattaat
    21001 acaacgccaa ttattgaaaa gcaggtaaag caatcagcgc cgccctgccc gattgtgatt
    21061 aatactcgtt cagatattga gctacgccag atcgcacaac gaatcacaca aacctgtcat
    21121 atcccggtac gaatcagcgc cgatgtctgg acctatctga acggtacagg cactggctca
    21181 acacaacaaa tgacaggctc aattccagca cctgatgcaa atggaatggt tcctctggcg
    21241 tcagtaggca gtcagccagt aaaagtctct tctggcggta catcactccc tgaactgaat
    21301 atctccgggc ttcctgccct actgcaaacc gtatcttcac ggctgggaat atcctggaaa
    21361 tacgataaag gtcgtatcac attcttctat ctggaaacgc gctcattccc gattacatat
    21421 atggacagca atgtggcata caactcaaaa gttgtgagcg gcacaatgtc ttccagtggc
    21481 agtaccggct ctaccagttc tggcggtatg actggcgacg catcaaatac gcaaactaca
    21541 accgtagaaa tgaaatcatc gctgtacaac gacctgaaaa gcgaagtttc ctcaatgctg
    21601 acaccaggta cagggcgcat gtatctgtcc acaggttcac tgacagtaac agataccccg
    21661 gatgtactgg acagcgttca ggagattgtg aacaagcgca ataatgaaat gagtcgtcag
    21721 gttgtgctga acgttgaaat cctgagtatc aagaaatcaa gccaggaaca ggcgggaatc
    21781 gactggaacg cagtatttaa tgatggtcat cttggtctga gtcttggtgg ctcatttggt
    21841 gatgccgccg agaatgttat taccagtggt gtatccatcg tggacggtaa gctggctggc
    21901 tcaaaagcat ttctgaaagc cctgtcgtca caaggcagcg tcagtgtcgt aacgcaaaac
    21961 tccgctgtga ctaaaaacct gacgcctgtc cctatgcaaa ttgccaatca gcagggcttc
    22021 attgaaagtg tcacaaccga ttctaccgcc aatgtgggat catccacgtc actgaatgcg
    22081 gcaacaataa cgaccggatt caatatgact ctgcttccat acattcagcc ggattcacaa
    22141 aacctgcaat tgctgttctc aatgagtctg tctgacaaac ctacttttga ggtgttcgaa
    22201 agtggcggca gtaaagcaca aacaccgaat gtggatttga aaacaattaa tcagactatt
    22261 gatctgaaat caggccagac agtcattctg agtggattcc agcaatcaaa ccggaaaacc
    22321 agtaaacaag gtgtaataac cccctccttc tttggacttg ggggtggcgt taacagcgaa
    22381 gacggcgaca cgattctggt cgttttgatt acaccaaatg tgcttaattc cggctccagt
    22441 gcagtaataa aaaatgtgaa ctaattaact gaaaactccc tgaaaaaaca cagggagttt
    22501 ttttggaggt cactttgtct gagaataatc agaataaccg gaatttcact tcggttatta
    22561 aaaacaagcg tgcttttttc tccggtctgg actggaaaac gcttccttca gaagaaaaga
    22621 atgcgcgtac attcgcaaga aaaaacgacg ctgaatattt tctctcctgt cagtatcagg
    22681 attcagaaaa tgaaaccaaa acgatggtgg catttatcag aaaagaggat cttcccacag
    22741 gggcgtcctc attctggtca ctggcactga tgataaaacc cctcatcgaa cctgacggat
    22801 acgctatctg cgaactcggt gatctttatg gctttgtttc ctgcgtcaat aacgttctcg
    22861 tcaacgatgt tgtaggtaac aaaagtcaga tcatgagtgc tctgacaaca tttcttgagt
    22921 ttaatgaaac accagaaccc ggctggaaac tataccagcc tgaatcatgg gatatcagcc
    22981 aggcactccc ctcactcact ctgtctgcgt tgattgacgt aaaaaaaccg ccgaaagagg
    23041 cggcatttac tcgcgtatcc cgaaaacgtc agttcatgat ttatggcgga agtgccattc
    23101 tggccatact gctctggaac gggatcacca tgtaccagga ataccgggaa aaagaagctg
    23161 cggcagaagc tgcacgcctt cgtctggcaa aagaaatggc agacaaacag gcaatacaga
    23221 ttgccccacc gtggcaacat ttacctgaaa taaaaccatt tattgataaa tgcattgata
    23281 aatgggatgc actgccctta tcgatcgccg gatggcgttt tgaccttgct gaatgcagca
    23341 catccggtaa tgacgggctg ttacgcacat catataagga actgagcggc gttacggtcg
    23401 aggacttcag cacacggata cgagaaatat tccaggggac aaccacagca acttttgtat
    23461 tgccagaagg cagcgccggg ggattttctc ttcctgtcag ttttgacgtt tcgcctgatc
    23521 caattacccc ggacacattg ccacaggcaa cagatattca ggaacgcctg acaacatttg
    23581 cacaaaaaat gaggttaaaa ctcacctggc aggaaattga aaacacaaaa acagatgaag
    23641 aaggccgtcc aataattctc ccgtggaacg agtatgaatt aatgattcag accagcaccc
    23701 cgccttcaat acttttcgct aactttcatg aacctgccgt tcgcttccag tatgcaggaa
    23761 taaaactgga agaaggaaga ctgaattatg agattaaagg tgcattctat gttaaaaata
    23821 attaaaccaa cgattatttc tgtctgtttt ttcagttcac ttgcttacgc aacagaatct
    23881 gaaagcacac caaaagacga aataaaatat tcatctccga ccattttaca ggtagagcag
    23941 attcaggctg aaacggtaat ttacgaagca aagctggcgc gtcagaaggc tgtcagtgaa
    24001 ttacgtaatc ttgatttgaa tggtgtagcg tccgcaacgc cagcaataac gccctcttcc
    24061 gggtattctt ctcttgcaga taccgatata cctgcgcaac gtgtagccgc taaatcactt
    24121 cgtgttattg aaatatttgg tacaccacaa caaatgtcag cacgaatttc tctcatggac
    24181 gggtcagtaa ctgatgtcaa ggtcgggcaa agtattccag gtacttctta taaggtggca
    24241 tcaatcactg gtaatggcgt ctggattcag aatggtacag agaaacgcca gcttattctt
    24301 taatgctatt gaaagagacg acacataatg tttttttctg acgcagacta tcccggaaaa
    24361 gatatttctg atgttgtata cgtcagtata tctgaaaaag acgataaacc tgttcatgtt
    24421 tatatcgctg ataacagtaa aggacagcgt gctgttcagg cttatattgg cactctgaat
    24481 gttaaatatc ctggaaaggt aaatataacc tggaccactc ttgatgttat cgcatcccgt
    24541 taccaggaca gcgaaagaag gtctgattca aactcatcac aacacttaaa cagcaaccag
    24601 gaaaaggtta ttagctacct ggcgaaagca aacaacctgg gaagttccga tttgcacatc
    24661 acacctggtc gggacggttc cgagttcact tatgtcgaag cacgcgtgca tggggaactg
    24721 gaaattctgg atgtcatccc gcgtaaagaa gggctggaat tgctgggtgc tgcctattct
    24781 ggaatgagcg atgttatcaa aggtacgcag tttgacccag caataccgca ggatgcacgt
    24841 atcgcagaga attttctgaa gcctgtaaat ctttttgggg cgcgctattc gcattatccg
    24901 tgcgtgggtg gtgtgtacgc ggtcttccgc ctgattaaag atgacagcga ggatattccc
    24961 actttcgaag aacttggcta catgccacaa caaatccaga caatcaggcg aatgttacaa
    25021 cgcccggaag gaattatcgt tttatccggc cctacagggt ccggtaagtc aacgactctg
    25081 agaacggctt cagaagccta tctgagcacg tttggtttca accataacga taatatgcgt
    25141 ctcccccgaa aacgcctttt caccattgaa tcaccgccgg agggccgcat tcccggcgca
    25201 atacagacgg cagtcaggga ttccgtcgat ggctgggttg acgcgattaa atccgctatg
    25261 cgacttgatc cagacgcgat actgaacggc gaaattcgtg atcacgcatc ggcattagca
    25321 gcaatcaaag cctcgatgac aggccacctt ctgttgacga ccttacacgc caacgatgcc
    25381 ctgaatatta ttgagcgcct tgaaatggag aatattcaag ccaggctgat tgcagatcct
    25441 cttctgttta tcggtctgct gagtcaacgg ctggtacaaa aactctgtcc ttactgtaag
    25501 cgcacttatg ctgaaatggc agacaaactg agtacagaag aacggaaact cattgaggaa
    25561 aattgcctgc cggaacaggt ccggttgcgc aatcttgacg gttgcgagca ctgttataaa
    25621 ggcgtaacgg gaaggacggt tatagcagaa gtcgtctcac ccgacgcacg tttttttcag
    25681 ttatatcgtc agtccgggcg tctggaagca aaatcatact ggcactatga acttggagga
    25741 attacccgta atcagcactt attacacctg attaatcatg gcctggtaga tccgctgtcc
    25801 gcacacttca tatcccccat tgacgaagat aaatactcaa tgctcccgga ggggacatgg
    25861 cgataaaaga tatatttgag aaactcaatt cgatagaaat tgatttctct ggaatatccc
    25921 actggctggc aaaatacaca ttcaggtatt ccagaataag tttatatgaa gatttatctg
    25981 gaatgttaaa tgatggcata aatgtaaagc gtgctcttac acacctgatt gatgtagaaa
    26041 cagactacgg gaagaaaacg gggtcttctg caacctacta tatttgttgt gagtgcctta
    26101 atgcactgaa taacacaggg gcaatatctt cagcattaaa aaaatatata aaggaggatg
    26161 aatatcaact gatatccagc ggtgaaattc gcggcgacct tgcgggaggg ttagttcagg
    26221 ccataaaaat tgtcagaagt cgttctgaaa tgacaatgac tcttgttatg tcgatgattt
    26281 atccatccgt actggttttt ggttgtctgg caaacatgta tatggtccac gataccataa
    26341 cacctgtttt cctttctctc gcaccgaaag agcgctggac aagttctatg cgattactga
    26401 cagatacgtc tggtttcctg atagaaaacg gaacgtatat tctctgcttc tgcatcgtgc
    26461 tgtttttcct gatcagatat tcgcttgcgc gttatacagg accgggccgt cagtatcttg
    26521 attacattcc cccctggtca ctttataaaa catttaatgg tgtaagtttt ctcttcaaca
    26581 tggcctccat gttaagtatt gatattcccg ttgcaaaagc actggaaagg ctggagaaaa
    26641 acagcactca aaacagatgg ctactcgaac gaataaaaga aatcaggaga tatgttttac
    26701 ttggacagtc acttgcaatg gcaatgaaaa gttgcggtta tgacttccca tcgaaattat
    26761 gcattaacaa actattactt cattctgaaa atgctgacaa tgcattgatg atgtctaact
    26821 atgctgataa atggctggag gaagcaaaag gaaaagtcaa atcaacagga gtctggataa
    26881 ctgtagtatc cgcattaatt gttttcgcat ttattgtaat gatgattact gctctctacg
    26941 atgtcagtaa cgtcctgcaa cactaatccc taatattttt aacctctcaa taaataagga
    27001 attatttatt atgtcttcta ttaatatttt aaatatgcgt tctgtttttt cttctctttc
    27061 tgcacgccgt aaaaaagaac aggacaaagg ggcaacactg atggaagtgt tattggttgt
    27121 tggtgttatt gttgtacttg cagcaagtgc atataaactc tattcaatgg tgcaatccaa
    27181 cattcaatca tccaatgaac aaaacaatgt cctgacagtg attgccaaca tgaaatcact
    27241 gaaattccag gggcgttata ctgacagtaa ctatattaag actctctacg cgcaaggatt
    27301 actgccatcc gatatgatcg cagatacaac tggcgcaagc gcgaaaaacc cgtggggcgg
    27361 ctccgttaca atcaccacct cttcggataa atactccttc aatgttgtag aggccaatgt
    27421 cccacagaaa aactgcatgg caatggtaaa cgccctgcgt tcttccagcg caatctccaa
    27481 aattaacaac acttccacat caaccgtcag tgcagcaact gtttgcgcat ctgacagcaa
    27541 tacgctgacc ttcagcactg attcctgatt tttcatgtga atcggataag cgccttcggg
    27601 cgcttatttt ttccatgttc acaggaggag tattgtgcgt tctgttcttt atatcctgct
    27661 atcatgtatt tcatcattaa tcttttcatt gcaacccgca tattcagcga caacgacccg
    27721 atttgatgcc tgctttgcac aagccgggca acgttacctg attgaccccc tgcttctgaa
    27781 aagcattgcc actgtagaaa gttcactcat accagaccgg ataaatcaca acaaagaaaa
    27841 aggaaaaata ttaagcaccg attacggact aatgcaaatt aattcatcac acatccctaa
    27901 cctgaaaaaa cttggcgtaa taaaagacaa atcagagctt attgataacc cctgtctcaa
    27961 tatacaaatt ggcgcgtgga ttctggcaac acattttcag aagtgtggca taaactggag
    28021 ttgtcttggc agttataacg ctggttttaa agaaagtaac gaacagaaaa gaatcaaata
    28081 cgcacgctat gtctacaata aatacatggt aaggtaactt atgctaaaca gcatatttaa
    28141 tacggcgata ctttgctgtg caaatatcgt ctgtcaatgc tacgcctaca atgaagttaa
    28201 attccgctta aataaactta atgtatctta taaaaccgga acagagaaat attactgtct
    28261 gatactgaca acgcttgccg ttctgtatct ttcattaaat cagctatcac tgattcaatc
    28321 cataatagtg attgttttct atgcattcct tacattaatg gcctgtatag accttctttc
    28381 atttctacta ccacgccttt ataccgtgac atttatcttc tccggcctgt tatatcagac
    28441 atggaataac aatatattgt ctggtttgtt ttgtgcaatg ctgatgttct tcattatgct
    28501 gtttgtaaga ctttactttg catataaaaa cggaacggaa agtttcggca tgggagatgt
    28561 gctacttatt gcaggaactg gcgtatggtt tccaacgcct gagattgcct gttcaattgt
    28621 atttattgcc gttatcggcg gaattatttt cttcactctt ggcggattaa acaagcaaaa
    28681 aaaatatatc ccattcggtc cgttcctttg tggcggaatg tttgtttact cactcgtacc
    28741 gggaatatta ttctgaggat taagagcaat gaaaaagaca gataaaggtg tatctctcct
    28801 tgaggtatta cttgtcatcg gcataatggt gatggttatc cctaaagttt atgaaaacat
    28861 tgaaaaccat ctgaataatg ttcgctggca gaatgcagca gaacacgcaa atacctataa
    28921 caccgctgtt cgtaattatg ttgctgacaa tgcatcaaca ttacttgctg gctctttacc
    28981 caaaacaatc acgccagcaa cactcattca gaaaggctat ctgaaatctg gtttttctga
    29041 atccaatttt ggacaaagct acattaccgg tattgcaaag aacagcaaaa catcccgtct
    29101 ggaagccctg acctgtagta atggcggtca gtccctgtca gaagccggaa tgcgctcggt
    29161 agcaagcatg attgaagggc ttggcggcta catcaacagc agcaagcagg cgattggcgc
    29221 gggtggcggc tggtctgaca cgccttcaaa ctatgggctg aactgcgcga ccgggcatat
    29281 tgcaatggca cttgttggcg ctgatttgca ggaaagcgac cgtttgtatc gttactccat
    29341 aactaatcgt cccgatctta acaggatgca tacggcgatt gatatgaaca gcaacaatct
    29401 gaacaacgtc ggcactttaa acggcaacgc cgccgcgctc tccggcgata tcagcgcacg
    29461 taacggtacg ttctctggcg caatttcagg aaatacagcc acaaccaacg gtgatatcac
    29521 ttcgaataat ggctggctgg tcacaaaaaa cagcaaaggc tggatgaaca gcacctatgg
    29581 tggcggctgg tatatgtccg attcgtcctg gttgcgcagc gtgaataata aagggattta
    29641 cactggcggc caggtaaaag gtggaactgt acgcgctgat ggacgcctct atacaggcga
    29701 gtatttacag cttgaaaaaa cagcaacggc tggcgcttcc tgctccccta acgggcttgt
    29761 aggccgcgac agtacagggg cgatactt
//
LOCUS       DAZFKF010000056        29473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFKF010000056 DAZFKF010000000
VERSION     DAZFKF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.056"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            92..3844
                     /gene="yfaL"
                     /locus_tag="ACHKA1_002976"
     CDS             92..3844
                     /gene="yfaL"
                     /locus_tag="ACHKA1_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO5680596.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQVWSIADGQWLVFSDTTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIELRVTNA
                     VFRNNIANDGKGGAIYTINNDIYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFEHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDAGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYKDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVSLAND
                     NQYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKPQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFETGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALDASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     TGAVSLQNGVAGDTLTVNGDYTGGGTLLFDSELNGDDSASDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIEVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFRGRWGTDGEW
                     MLGIVGGYSDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDNWLQYA
                     WFSNDVSEHEDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3985..4707)
                     /gene="ubiG"
                     /locus_tag="ACHKA1_002977"
     CDS             complement(3985..4707)
                     /gene="ubiG"
                     /locus_tag="ACHKA1_002977"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO5680597.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4854..7481
                     /gene="gyrA"
                     /locus_tag="ACHKA1_002978"
     CDS             4854..7481
                     /gene="gyrA"
                     /locus_tag="ACHKA1_002978"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO5680598.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7630..9318
                     /gene="yfaA"
                     /locus_tag="ACHKA1_002979"
     CDS             7630..9318
                     /gene="yfaA"
                     /locus_tag="ACHKA1_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO5680599.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNNETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DEEQDTEATAIASDLLSGKKRWQASFGLEERTAGKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFLALNDESASVDNSFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9315..9938
                     /gene="yfaT"
                     /locus_tag="ACHKA1_002980"
     CDS             9315..9938
                     /gene="yfaT"
                     /locus_tag="ACHKA1_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO5680600.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGNAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9962..14476
                     /locus_tag="ACHKA1_002981"
     CDS             9962..14476
                     /locus_tag="ACHKA1_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO5680601.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLDGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVTLNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFDKPGNYNLTLRDKDGLILAGLSHAVSGKGSM
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWITL
                     QRLNDTQYEARVPVNSTFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSLLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPSAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEAPRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLTHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14477..16126
                     /gene="yfaQ"
                     /locus_tag="ACHKA1_002982"
     CDS             14477..16126
                     /gene="yfaQ"
                     /locus_tag="ACHKA1_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO5680602.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLNEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQAMAQAGQGDRYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16131..16907
                     /gene="yfaP"
                     /locus_tag="ACHKA1_002983"
     CDS             16131..16907
                     /gene="yfaP"
                     /locus_tag="ACHKA1_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO5680603.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIHGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17182..20031
                     /gene="rcsC"
                     /locus_tag="ACHKA1_002984"
     CDS             17182..20031
                     /gene="rcsC"
                     /locus_tag="ACHKA1_002984"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO5680604.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20117..20767)
                     /gene="rcsB"
                     /locus_tag="ACHKA1_002985"
     CDS             complement(20117..20767)
                     /gene="rcsB"
                     /locus_tag="ACHKA1_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO5680605.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20812..22101)
                     /locus_tag="ACHKA1_002986"
     CDS             complement(20812..22101)
                     /locus_tag="ACHKA1_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5680606.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITAN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPGKNQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQRLNKA"
     gene            complement(22135..22389)
                     /gene="xisR"
                     /locus_tag="ACHKA1_002987"
     CDS             complement(22135..22389)
                     /gene="xisR"
                     /locus_tag="ACHKA1_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO5680607.1"
                     /translation="METIIYQITPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEY
                     RHYAAEGEPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            complement(22397..22543)
                     /locus_tag="ACHKA1_002988"
     CDS             complement(22397..22543)
                     /locus_tag="ACHKA1_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680608.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     LKIV"
     gene            complement(22564..22806)
                     /locus_tag="ACHKA1_002989"
     CDS             complement(22564..22806)
                     /locus_tag="ACHKA1_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680609.1"
                     /translation="MGEMVDINDELVRTVITVDDGCDHTQRFIWMMNTRRNIRNKVFR
                     HEPKPVRVTKIRIEARKRRKYRPRKAWAPAQPDELA"
     gene            complement(22799..23701)
                     /locus_tag="ACHKA1_002990"
     CDS             complement(22799..23701)
                     /locus_tag="ACHKA1_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680610.1"
                     /translation="MKEADLKALRDAAQNTVYGDKGCDLEAFHSLATPPVILALISHI
                     ENLEKKLDEIVTSEISPKAWLNNLTGEVCSIDAMPDARNDRETYTPLYPAWQRIAAAD
                     DNGRVAWMYTHKKAGYRVPTMTSWESLAEERGGSLDKYIVSPLFTVPQIPPVRTEQDA
                     YPDVQVSWDATRHYVNGWNACRQAMLTTAEKVLDVRIPEYIGRWLSAALHDQNSCEEM
                     KNDIRRFLAEYHHVPSKYNGWRLVPDKLTEQMKYRIVPLLATIKPRSVDEYDRSGENA
                     QFLEWVWTQLMEATKFAYEGSVNG"
     gene            complement(23698..23922)
                     /locus_tag="ACHKA1_002991"
     CDS             complement(23698..23922)
                     /locus_tag="ACHKA1_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680611.1"
                     /translation="MIISFTDYGEQAKLIITSSRLTLRKHNRVVDAVLLCTPDITESR
                     SGFFLVKSSLSGKTRDILRAYKTATRESER"
     gene            complement(23939..24301)
                     /locus_tag="ACHKA1_002992"
     CDS             complement(23939..24301)
                     /locus_tag="ACHKA1_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO5680612.1"
                     /translation="MNSTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITCAIENLYENLPYEEAQDEYTGETNIMATIQIGDLACTDEDMLGEAWLA
                     GMLIAAEIISIEPAGTLS"
     gene            complement(24294..25160)
                     /locus_tag="ACHKA1_002993"
     CDS             complement(24294..25160)
                     /locus_tag="ACHKA1_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO5680613.1"
                     /translation="MKKSTELVPVAARDLQIIEYRGQRVVTTEQLAAGYGATPIRIRQ
                     NHSRNEERFVVGKHYFLLEGDELRELKHRVSQSYSVKIASNVRSLILWTERGAANHAK
                     LLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVIEAEERAEAMALENKTLS
                     ATVDSLEKHFSKGMTVPQFCRALNGVNTSQIMWWLSGRKWVFNEQRDPEKTPRWRTAS
                     YARDRYLTEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFYMNGELPMKKNWNGEFKH
                     DKAIYTPEAEHE"
     gene            complement(25184..25768)
                     /locus_tag="ACHKA1_002994"
     CDS             complement(25184..25768)
                     /locus_tag="ACHKA1_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680614.1"
                     /translation="MTATEQLFFLTAEGIHTPYGLLDINKALEMLDEGCFDEPIWHGL
                     EIVCCISEAEEKGMTGELAPEKTIMYWRWLYTAMFFSEQLTRNGWTEVLNENGGTDKG
                     TMYRGHNGGMTIYLKPLRLAMQNYMEWALIETYGAESGLLKSILMYKAFTGALGDGEK
                     TGITMEGYRAMHMILDGLISEATDNSEPETRVFH"
     gene            complement(25755..26315)
                     /locus_tag="ACHKA1_002995"
     CDS             complement(25755..26315)
                     /locus_tag="ACHKA1_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680615.1"
                     /translation="MKEEPEIIEINSEEFTENEWLTETDPRVLALTMEGYIVFPPFFS
                     DAEYSAINNAGIDRAFHKYELDCGPVYRVCRHGQVKKCFTRKTAIIHLAHFMTTKVFE
                     SSGFEKRLPDQRLMHPEYGEVFHPGEITPEYYRAHGRCFHRLLRILARRKQFDAWMEK
                     YNAWIDQYCRLVAQRPFKKRANHDCN"
     gene            complement(26723..27085)
                     /locus_tag="ACHKA1_002996"
     CDS             complement(26723..27085)
                     /locus_tag="ACHKA1_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680616.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGRKDGETAIRTAYQDAVGINQHGEYLTESGKLIIDELL
                     NECIDYIKEGHITGCVNH"
     gene            complement(27260..27460)
                     /locus_tag="ACHKA1_002997"
     CDS             complement(27260..27460)
                     /locus_tag="ACHKA1_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680617.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCLIR
                     SMNKTCEKAYEYVDALRTNKGN"
     gene            complement(27457..27648)
                     /locus_tag="ACHKA1_002998"
     CDS             complement(27457..27648)
                     /locus_tag="ACHKA1_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680618.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            complement(27650..28027)
                     /locus_tag="ACHKA1_002999"
     CDS             complement(27650..28027)
                     /locus_tag="ACHKA1_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5680619.1"
                     /translation="MSTSQLMLIEQVESLLHESLESVSERNRHVLLEVGIEKIQQLKH
                     ILLPPRNKKDCRDGIRTQRESCGLSTAELATLLGVDEETIIQWENGKAEPLASQVIPL
                     ANILDCDPMWLLTSFHSDTTKES"
     gene            complement(28113..28772)
                     /locus_tag="ACHKA1_003000"
     CDS             complement(28113..28772)
                     /locus_tag="ACHKA1_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO5680620.1"
                     /translation="MDHDSFGGRLLMRRVQLGMSQDDLAKLTGVSRVTISKIELGDSQ
                     DTRSSNLFRIADALKCSPKWLLDGSAPSPTDSIQNVRNPKPVSPNRYRYPKLTWVSAG
                     PWNCEDYIAYPEEWIGSDIYAGDHGFWLEVKGDSMTSQNGEFSIFEGMNILVNPDKEV
                     FSGKYVVARLKNSGEATLKQYIEDAGQCFLKPLNPRYPLIPINGDCEIIGVVVEMRMQ
                     I"
     gene            29112..29405
                     /locus_tag="ACHKA1_003001"
     CDS             29112..29405
                     /locus_tag="ACHKA1_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680621.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYAEV
                     YEVTFDSEPVSFRKENRARHEANVRLRRFVETHGRALQGYTTQPPLAGTQQRT"
BASE COUNT         7075 a         7121 c         7712 g         7565 t
ORIGIN      
        1 atcgggttgg acaatttaca aaagaaagat aacccatatt gtataatgcg tttaaatatg
       61 ccgtcttata taaaatgatg gtcagattaa tatgcggatt atctttctac gcaaggagta
      121 tttatctcta ctcccgtcaa tgattgcatc tcttttctct gctaacggtg tcgcggcggc
      181 cattgattta tgccagggat atgatatcaa agcgagttgt cacgccagca ggcaaagcct
      241 ttcaggcatt acgcaggtct ggagtatcgc cgatgggcaa tggctggttt tttcggatac
      301 gaccaataat gccagcggtg gggccgtatt tttgcaacaa ggagcggaat ttacattatc
      361 accagaaaat gaaactggaa tgactctgtt tgccaataac accgtttcag gagaatataa
      421 taacggcggg gcaatatttg ctaaagaaaa ctcaacgctg aatcttacgg atgttatttt
      481 ttctggtaac gtcgcaggcg gctatggtgg cgcaatctat tcttctggta ctaacgatac
      541 cggtgccatc gagttacgtg tcactaacgc cgtgtttcgc aataacatcg ctaatgacgg
      601 caaaggtggt gcaatttata ccatcaataa tgatatctat ttaagtgatg atgtttttaa
      661 caataaccag gcatatacat caacaagtta cagtgatggc gatggcggcg caatcgatgt
      721 cacagataat aatagcgaca gcaagcatcc ttcaggttat acgataataa ataacactgc
      781 ctttacaaat aacactgccg aaggttatgg cggggcgata tataccaata gcgcgacggc
      841 tccctatctt attgatattt ctgttgatga cagctacagc cagaacggag gcgtgttagt
      901 cgatgagaac aatagcgcag caggctatgg agatggtcct tcctctgcgg cgggtggctt
      961 tatgtatctc ggcttaagtg aagttacatt tgatattgcc gacggaaaaa cgctggttat
     1021 tggcaataca gagaatgacg gagctgttga ctctattgct ggtaccgggt taatcaccaa
     1081 aacaggttcc ggcgatctgg tacttaatgc agataacaat gactttactg gtgagatgca
     1141 gattgaaaac ggtgaagtta ccctgggccg cagcaactcc ctgatgaatg tcggcgatac
     1201 gcattgccag gacgatccgc aagactgcta cggtctgacg atagggagta ttgataagta
     1261 ccagaatcag gcagagctaa atgttggctc cacccaacaa acctttgagc actcattgac
     1321 gggctttcag aatggcactt taaatatcga tgctggtggc aatgttactg ttaatcaagg
     1381 cagttttgct ggcaccatcg aaggtgctgg tcagctcacc attgcgcaaa acggcagcta
     1441 tgtgctggcg ggggcgcagt cgatggcgct aaccggcgat atagtggtgg atgctggtgc
     1501 ggtgctttcg ctggaaggcg acgcggcaga tcttgccgct ctccaggacg atccgcagtc
     1561 gatcgtgtta aacggcggtg tgctcgatct ctctgatttc tccacctggc agagcggtac
     1621 atcatacaaa gatggccttg aagtcagtgg cagcagcgga acggttatcg gcagtcagga
     1681 tgtggtagat cttgcaggcg gaaacgatat gcatatcggc ggcgacggga aagatggcgt
     1741 ctacgtggtg atcgatgcgg gtgacgggca ggtcagcctg gcaaatgaca atcaatacct
     1801 cggcacaacg caaatcgctt ccggtacgct gatggtgagc gacaactcgc agcttggaga
     1861 tacccattat aaccgccagg ttatctttac cgataagcca caagaaagcg tgatggagat
     1921 tactgccaat gtcgatactc gctctacaac gactgagcat gggcgtgata ttgaaatgcg
     1981 cgccgacggt gaagtggcag ttgatgcggg ggtagacacg cagtggggcg cactgatggc
     2041 tgacagcagc gggcagcatc aggatgaggg tagcacattg acgaaaacgg gggcgggtac
     2101 gctggagctg accgccagcg gtacaacgca gtcggcggta cgtgtcgaag aaggcaccct
     2161 gaaaggtgat gttgctgata tccttcctta tgcttcatca ctgtgggttg gtgacggggc
     2221 aacgttcgag actggcgcgg atcaggatat tcagtctatc gatgctactt ccagcggcac
     2281 tatcgacatc agcgatggta cggttttgcg cctgaccggg caggatactt ccgtcgccct
     2341 tgatgcctca ctgtttaacg gcgatggtac gctggtgaat gccaccgatg gcgtgacgtt
     2401 gacaggtgag cttaatacca accttgaaac tgacagcctg acttatcttt ccgacgtaac
     2461 agttaatggc aatctgacca atacgaccgg tgcggtcagc ctgcaaaatg gcgtcgctgg
     2521 cgatacgctg acggtaaacg gtgattacac cggcggcggt acgttactgt tcgacagcga
     2581 attaaacggc gatgactcag caagcgacca gttggtgttg aacggtaata ctgctggcaa
     2641 cacaactgtg gtggttaact ccattacagg gattggtgag ccgacatcga caggcattga
     2701 agtggttgat ttcgcagctg atcccacgca gtttcaaaac aatgcgcagt tcagtctggc
     2761 aggcagcggc tacgtcaata tgggagcgta tgactacacg ctggtggaag ataacaacga
     2821 ctggtatctg cgatcgcaag aagtaacgcc gccatcgcca cctgatccag acccgactcc
     2881 cgatcctgat cccacgccgg atcctgatcc aacacccgac ccggaaccta cgcctgctta
     2941 ccagccggtg ttgaatgcca aagttggcgg ttatctcaat aacctgcggg cggcaaatca
     3001 ggcgtttatg atggagcgac gcgatcacgc aggtggcgat ggtcagacgc tgaatttacg
     3061 tgttatcggc ggagattatc attacacagc ggcagggcaa ctggctcagc atgaagacac
     3121 ttctacggtg cagcttagcg gtgacctgtt tagggggcgc tggggcacgg atggcgagtg
     3181 gatgcttggg attgttggtg gctacagcga taaccagggc gacagccgct cgagtatgac
     3241 cggaactcgc gccgataacc agaaccacgg ttatgccgtt gggctaacct caagctggtt
     3301 tcagcacggt aatcagaagc agggggcctg gctggataac tggttgcagt acgcgtggtt
     3361 tagcaatgat gtttctgaac atgaagatgg cgtggatcat taccattcgt cggggattat
     3421 cgcctcgctg gaagcggggt atcagtggtt accggggcgt ggtgtggtga ttgaaccgca
     3481 ggcgcaggtg atttatcagg gcgtgcagca ggatgatttt accgccgcta accgtgcgcg
     3541 cgtgtcacaa tcgcagggtg atgatattca gacgcggctg ggtttacaca gcgaatggcg
     3601 taccgctgtt catgtcatac caacattaga tctgaattat tatcacgatc cccattcgac
     3661 ggaaattgaa gaggatggca gcactatcag tgacgatgcg gtgaagcaac ggggtgaaat
     3721 aaaagtggga gtcacgggca atatcagtca gcgagtgtcc ctgcgcggca gcgtggcgtg
     3781 gcagaaaggg agtgatgatt ttgcccagac ggcagggttt ttgtcgatga cggtgaaatg
     3841 gtaatcgctg tctgaccatc actattgccg gatgcggcgt aaacgcctta tctggcctac
     3901 ggagagcatt tgtaggcctg ataagacgcg gcaagcgtcg catcaggcat tggcgcggtt
     3961 gccggatgaa tccaaacaac aacctcactt attctgcgtg tgcagcatat agttcacatc
     4021 cacgccgggg ccgagtttaa aagtattagt gatcgggttg taatgtagcc cggttatatg
     4081 tcgctcttta aggcttgtct gatctaccca gccaagcagc tctgccggtt taataaactt
     4141 cttcacatca tgcgtgcctt tgggcaccat gcgcaaaata tattccgcgc caaccaccgc
     4201 catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc cgcccggttt
     4261 caccagttgc gcacaggctc tgaccactga ctgcggatcg gggacgtgct ccagcatctc
     4321 catgcaggtc accacgtcat actgcccggc atgttttgcc gcgtgctctt ccacagtttc
     4381 ttgcacgtaa tccacttgaa tgccgctttc cagcgcgtgc agttttgcca cctgcaatgg
     4441 ctcaaagccc atatccagac cggtcaccgt cgcaccttcg cgcgccatac tctcggccag
     4501 aatgccgccg ccacaaccga catcgagcac ctttttgcca aataagccgc cagcacgctc
     4561 ggcaatatag cccagacgca gcgggttaat gcggtgcagc ggtttgaact caccttccag
     4621 atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt ctacgttatg
     4681 gtttaccggc gatttttcgg cattcattgg cacttctact ccgtaattgg caagacaaac
     4741 gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaatc tgcgcggctg
     4801 tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt tagatgagcg
     4861 accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc tcctatctgg
     4921 attatgcgat gtcggtcatt gttggccgtg cgctgccaga tgtccgagat ggcctgaagc
     4981 cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg aacaaagcct
     5041 ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc catggtgact
     5101 cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt tacatgctgg
     5161 tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca atgcgttata
     5221 cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa aaagagacgg
     5281 tcgatttcgt tgataactat gacggcacgg aaaaaattcc cgacgtcatg ccaaccaaaa
     5341 ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca accaacatcc
     5401 cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat gatgaagaca
     5461 tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg gcggcaatca
     5521 ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgcggcaag gtatatatcc
     5581 gcgcccgtgc tgaagtggaa gttgacgcca aaaccggacg tgaaaccatt atcgtccacg
     5641 aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa ctggtaaaag
     5701 aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac ggtatgcgca
     5761 tcgtgattga agtgaaacgc gacgcggtcg gtgaagttgt gcttaacaac ctctactccc
     5821 agacccagtt gcaggtttct ttcggtataa acatggtggc attgcaccat ggtcagccga
     5881 agatcatgaa cctgaaagac atcatcgcgg cgtttgttcg tcaccgccgt gaagtggtga
     5941 cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc cttgaagcat
     6001 tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat gcgccgacgc
     6061 ctgcagaagc gaaaactgcg ctggttgcta atccgtggca gctgggcaac gttgccgcga
     6121 tgctcgaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca gagttcggcg
     6181 tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg gatctgcgtt
     6241 tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa gagctgctgg
     6301 atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg gaagtgatcc
     6361 gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact gaaatcaccg
     6421 ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg gtcgtgacgc
     6481 tctctcacca gggctacgtt aaatatcagc cgctttctga atacgaagcg cagcgtcgtg
     6541 gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac cgactgctgg
     6601 tggcgaacac tcacgatcat atcctgtgct tctccagccg tggtcgcgtc tattcgatga
     6661 aggtttatca gttgccggaa gccactcgtg gcgcgcgcgg tcgtccgatc gtcaacctgc
     6721 tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc ggtgaccgag tttgaagaag
     6781 gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc ctcaccgaat
     6841 tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgacggc gatgagctga
     6901 tcggcgttga tctgaccagt ggcgaagatg aagtaatgct gttctccgcc gaaggtaaag
     6961 tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc ggtgttcgcg
     7021 gtattcgttt aggcgaaggt gataaagtcg tctctctgat cgtgcctcgt ggcgatggcg
     7081 caatcctcac cgcaacgcag aacggttatg gtaaacgtac cgcagtggcg gaatacccga
     7141 ctaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt aacggtttag
     7201 ttgttggcgc ggtgcaggta gatgactgcg accagatcat gatgatcacc gatgccggta
     7261 cgctggtacg tactcgcgtt tcggaaatca gcatcgtggg ccgtaacacc cagggcgtaa
     7321 tcctcatccg tactgcggaa gatgaaaacg tggtgggtct gcaacgcgtg gctgaaccgg
     7381 ttgacgagga agatctggac accatcgatg gcagtgctgc ggaaggagac gacgaaatcg
     7441 ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc ttcatgccaa
     7501 aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct gggcttttgt
     7561 cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct atacacaagg
     7621 aatgtggcaa tgagtggtga aaaaaaggcg aaaggctggc ggttctatgg tcttgtaggt
     7681 tttggcgcaa tagtactgct ttccgctggc gtctgggcgt tgcaatatgc tggcagtggg
     7741 ccagaaaaaa cgttgtcgcc gctggtggtg cacaacaatc tgcaaatcga tctcaatgag
     7801 ccggacctct ttctcgacag cgactctctg agccagcttc ccaaagatct cctcaccatt
     7861 ccgtttctcc acgatgttct gagtgaagat ttcgttttct attatcagaa tcatgccgat
     7921 cgtctgggca ttgaaggcag cattcgtcgc attgtctatg aacacgatct cacgctgaaa
     7981 gataaactct tttcgtcact cttagatcag cccgcacagg cggcgctatg gcacgataaa
     8041 caaggccatc tttcacatta tatggtgctt atccagcgta gtggcttaag caagctgctg
     8101 gagccattgc tgtttgccgc taccagcgac agccagttaa gtaaaacgga aatcagtagc
     8161 atcaagctaa ataatgaaac tattcctgtt tatcagttgc gctataacgg caataacgcc
     8221 ctgatgttcg cgacatatca ggacaagatg ctggtgtttt ccagcacgga tatgctgttt
     8281 aaagatgaag agcaggatac cgaagctacg gcgatcgcaa gtgatttgtt gagcggtaaa
     8341 aaacgctggc aagccagctt tggcctggaa gagcgtactg ccggaaaaac accagtacgc
     8401 cagcgcatcg tggtcagcgc caggttgctg ggttttggct accagcgatt aatgccttct
     8461 tttgccggag tgcgtttcga aatgagcaac gacggctggc atagctttct ggcgttaaat
     8521 gatgaatccg ccagcgtaga taacagtttc gattttacgc cggtctggaa cagtatgcct
     8581 gccggggcca gcttctgtgt ggcggtgccg tattcacacg gtattgccga agagatgctt
     8641 tcgcacatca gccaggaaaa cgacaagctg aatggggcgt tagacggtgc cgcggggctg
     8701 tgctggtatg aagactcaaa attgcaaacc ccgctgtttg tcggtcagtt tgatggcact
     8761 gccgaacagg cgcaattgcc agggaaactg tttacgcaaa atattggtgc gcacgaaagc
     8821 aaagcgccag aaggtgtttt gccggtaagc cagactcagc agggcgaagc gcaaatctgg
     8881 cgtcgcgaag tgagttcccg atacggtcag tatccgaaag cgcaggcggc gcaacccgat
     8941 caattaatgt cggattattt tttccgcgtg tcgctggcga tgcaaaacaa aacgctgctt
     9001 ttctccctcg atgacacgct ggttaataac gccctgcaaa cactgaataa aacccgcccg
     9061 gcaatggtgg atgtaatacc cactgatggc atcgttccgc tctatatcaa tccacaaggc
     9121 gtggcgaaac tgctgcgtaa cgaaacgttg accagtctgc cgaagaatct cgaaccggtt
     9181 ttttataacg ccgcacaaac tttattaatg ccgaagctgg acgctttatc tcaacaaccg
     9241 cgttatgtca tgaagctggc ccagatggaa cccggtgccg cctggcagtg gctacccata
     9301 acctggcagc cgctatgagg cacgggctgc tggcgctgat ttgctggctg tgttgtgttg
     9361 ttgcccatag cgaaatgctg aatgtcgaac aatccgggct gtttcgcgcc tggtttgtac
     9421 gcattgcaca agaacagctc cgccaggggc caagtccacg ctggtatcag caggattgtg
     9481 cgggcctggt gcgatttgcg gcgaacgaga cgctgaaagt tcacgacagt aaatggctaa
     9541 aaagtaacgg tttatctagc cagtatttgc cgccagagat gacgctaaca cctgaacagc
     9601 gtcaactggc gcaaaactgg aatcagggga acgggaaaac cggcccctac gtgaccgcga
     9661 ttaatttgat tcagtacaac agccagttta ttggccagga cataaaccag gcgctgcctg
     9721 gcgatatgat ttttttcgat cagggcaatg cccagcactt aatggtctgg atggggcgtt
     9781 acgtcatcta ccacaccgga agcgccacga aaactgacaa cggaatgcgc gcagtcagtc
     9841 tgcaacaact tatgacatgg aaggacaccc gatggatacc caacgattcc aatcccaatt
     9901 tcattggcat ttatcgttta aattttctgg cgcgatagca gcgtgcttgt ccctgtctct
     9961 tgtgggaaca gggcttgcta atgctgatga ttcgcttcct tccagtaact atgcgccgcc
    10021 cgcaggggga acattctttt tgcttgctga cagcagtttt agcagcagtg aagaggcgaa
    10081 agtgcgactg gaagcgccgg ggcgtgatta tcggcgctat cagatggaag agtacggcgg
    10141 cgtggacgtt cgcctgtatc gtattcctga cccgatggca tttttgcgcc agcagaaaaa
    10201 cctgcatcgc attgtggtgc aaccgcaata tttgggcgac gggctgaaca atacgctgac
    10261 ctggctgtgg gataactggt acggcaaatc tcgccgcgtg atgcagcgta ctttctcttc
    10321 tcagtcacgg cagaatgtga ctcaggcatt acccgaatta catctcggca atgccattat
    10381 taaaccttcc cgttatgtac agaacaacca gttttccccg ctgaaaaaat atcccttggt
    10441 ggaacagttc cgttatccac tatggcaggc taaaccggtc gagccgcagc aaggggtaaa
    10501 actggacggc gcatccagca atttcatctc gccgcagccg ggtaacattt atattcctct
    10561 cggccaacaa gagccgggac tgtacctcgt cgaggcgatg gttggtgggt atcgggcgac
    10621 gacggtggtg tttgtttccg ataccgtggc gcttagcaaa gtgtcaggca aagagcttct
    10681 ggtgtggacc gcgggtaaaa aacagggtga agcgaagccc ggctcagaga tcttgtggac
    10741 tgacggtctt ggcgtgatga cccgcggtgt gaccgatgac agcggtacct tgcagttaca
    10801 acatatatcg ccagaacgtt catacattct gggtaaggat gctgaaggcg gcgtttttgt
    10861 ctccgagaac ttcttctacg aaagcgaaat ctacaacacc cgcttgtata tttttaccga
    10921 tcgcccgcta tatcgcgcag gcgatcgtgt cgatgttaaa gtgatgggcc gcgagttcca
    10981 cgatccgttg cattcatccc ccatcgtcag cgccccggcg aagctttcgg tgctggacgc
    11041 taacggcagt ctgttgcaaa ccgtcgatgt cacgctggat gcgcgcaatg gcgggcaggg
    11101 aagtttccgc ctgccagaaa atgccgttgc cggaggttat gagttacgtc ttgcttaccg
    11161 caatcaggtc tatagcagta gttttcgcgt agccaactat atcaagccac atttcgagat
    11221 tggtttagct ctcgacaaaa aagagttcaa aactggcgaa gcggtcagcg gcaaactgca
    11281 acttctctac ccggatggcg agccggtaaa aaatgcccgc gtgcagttaa gtttgcgcgc
    11341 tcagcaatta tcaatggtcg gtaacgattt gcgttatgcc ggacgtttcc ccgtgtcgct
    11401 ggaaggcagt gaaacggtgt ccgacgccag cggtcatgtg acgttaaatc tccccgccgc
    11461 cgataaaccg agccgctatt tgttaaccgt ctccgccagt gacggcgcgg cgtatcgcgt
    11521 caccaccacc aaagagatcc tcattgaacg cggcctggcg cattactctt taagtaccgc
    11581 cgcacaatac agtaatagcg gcgagtcggt tgtgttccgt tatgccgcgc tggaatcttc
    11641 aaaacaggtt cctgttacgt atgaatggtt gcgtctcgaa gaccgcacga gccatagcgg
    11701 agagctaccg tctggcggca aatcctttac cgtcaatttc gataaacctg gcaactacaa
    11761 tctgacgtta cgcgataaag acggcttaat tctcgctggg ttaagtcatg ccgtcagcgg
    11821 taagggcagc atgtcgcata ctggtacggt agatatcgtg gcagataaaa cgctgtacca
    11881 gccaggcgaa accgcgaaga tgttgattac ctttccagaa ccaattgatg aagccttgct
    11941 gacgctggaa cgcgatcgcg tggaacagca gtcgctgctt tcgcacccgg caaactggat
    12001 aacgctacaa cgcttaaacg atacccagta tgaagcccgc gttccagtga acagtacctt
    12061 tgcgcccaac atcacttttt cggtgctgta cacccgtaac gggcagtaca gttttcagaa
    12121 cgcagggatc aaagtcgccg ttccccagtt agatattcgg gtgaaaacgg ataaaaccca
    12181 ctaccagccg ggcgaactgg tcaacgtcga attaacctcg ttgctgaaag gtaaaccggt
    12241 ttctgcgcag ctaacggttg gtgtggtcga tgaaatgatc tacgcgctac aaccggaaat
    12301 cgcgccgaat atcggcaaat ttttctatcc cctggggcga aacaatgtgc gcaccagttc
    12361 cagcctgtca tttatcagct atgaccaggc gctctccagc gagcctgttg cgcctggcgc
    12421 gactaaccgg agcgagcggc gagtaaaaat gctggaacgt ccacggcgtg aagaggtgga
    12481 taccgctgca tggatgccgt cgctaacaac ggataaacaa ggcaaagcgt actttacgtt
    12541 cctgatgccg gattcgttaa cgcgctggcg cattaccgcg cgtgggatga acggcgacgg
    12601 gctggtcggg caggggcgtg cttatctgcg ttcggaaaaa aatctctaca tgaagtggag
    12661 tatgccaacg gtgtatcgcg tgggcgacaa accgtcggca ggactgttta tcttcagtca
    12721 gcaggataac gaaccggtgg cgctggtgac taaatttgca ggcgctgaga tgcgccagac
    12781 gctgacgctg cacaaagggg cgaattatat ttcgctggcg cagaacattc agcaatctgg
    12841 cttgttaagc gcagaactgc aacaaaatgg gcaagtgcag gacagcatta gcacaaaact
    12901 gtcttttgtg gataacagct ggcccgttga acagcagaaa aatgtcatgc tcggtggtgg
    12961 cgataacgcg ctgatgttgc ccgagcaggc gagcaatatc cggctacaaa gtagtgaaac
    13021 gccgcaggag attttccgca acaatcttga tgcgttagtc gatgaaccgt ggggtggggt
    13081 gatcaacacc ggtagccgtc tgatcccgct cagtctcgcc tggcgttcgc ttgccgatca
    13141 tcaaagtgcc gccgctaacg acattcgtca gatgattcag gataaccgtc tgcggctgat
    13201 gcaactggcg gggcccggag cgcgctttac ctggtggggt gaagatggca atggtgacgc
    13261 cttccttacg gcatgggcat ggtacgccga ctggcaggcc agccaggcgc tcggcgtaac
    13321 gcaacaaccg gaatactggc agcatatgct cgacagttat gccgagcagg cagataacat
    13381 gccgttattg catcgggcgc tggtgctggc gtgggcacag gagatgaatc tgccgtgcaa
    13441 aacgttgttg aaagggttgg atgaagctat cgcccggcgc ggaactaaaa ctgaagattt
    13501 ctctgaggaa gccccccgcg atatcaatga cagcctgatc ctcgatacac cggaatctcc
    13561 actggcagat gcggtggcaa acgtcttaac catgacgttg ctgaaaaaag cgcagttgaa
    13621 gtccacggtg atgccacagg ttcagcaata tgcgtgggat aaagcggcaa acagcaatca
    13681 gccgctgacg cacacggttg tgctgctcaa tagcgggggc gacgctaccc aggcggctgc
    13741 tattttaagt ggtttgaccg ctgagcaatc tactattgag cgcgcgctgg ccatgaactg
    13801 gctggcgaaa tatatggcga caatgcctcc agttgttttg cctgcgcctg cgggcgcatg
    13861 ggccaaacat aagttaactg gagggggcga atactggcgt tgggttggtc agggcgtgcc
    13921 ggacattctc tcttttggtg acgaattatc gccgcaaaat gtgcaggtcc gctggcgtga
    13981 accggcaaaa acggctcaac aaagtaacat tccggtgacc gttgaacgcc agttgtatcg
    14041 gcttatcccc ggtgaagaag agatgagctt tactctgcag ccggtgacca gcaatgagat
    14101 tgacagcgat gcgctgtatc tcgatgaaat tacgcttacc agcgagcagg atgcagttct
    14161 gcgctatggt caggtggaag taccgctccc gccgggagcc gacgttgagc gcacaacatg
    14221 gggcatttca gtcaataaac ccaacgccgc gaaacagcag gggcaattgc tggaaaaagc
    14281 gcgtaatgaa atgggcgaac tggcctatat ggtgccggtg aaagaactga cgggaacggt
    14341 cactttccgc catttgctgc gcttctcgca aaaagggcaa ttcgttctgc ctcctgctcg
    14401 ttatgtgcgt tcctatgcac ctgcacagca aagtgttgcg gcagggagtg aatggaccgg
    14461 gatgcaggtg aaataagtga actggcgcag aatcgtctgg ctattggcgt tggttactca
    14521 gccaacgctt gcggaggaga cgccgttaca actggcgctg cgcggtgcgc agcacgatca
    14581 actgtatcag ctgtcgtcat caggcgtgac caaagtctca gcattacctg actcgctcac
    14641 cacgccgtta gggagtttgt ggaaactgta tgtttacgcc tggttggagg atacccatca
    14701 accggagcag ccctaccagt gtcgcggaaa ctccccggaa gaggtttatt gttgtcaggc
    14761 tggggagagc ataacgcgcg ataccgcgct ggtacgttcc tgtgggctct attttgcgcc
    14821 gcagcggttg catattggcg cagatgtgtg ggggcaatac tggcaacagc gtcaggcacc
    14881 tgcgtggtta gcgtctctta ctacgctgaa gccagaaacc tcggtaacgg ttaaatcgct
    14941 gcttgattca ttagcgacgt tacctgcgca aaacaaagct caggaagtct tactggatgt
    15001 ggtgctggac gaggcgaaaa tcggtgtcgc atccatgctc ggtagtcggg tacgggtgaa
    15061 aacctggagc tggtttgccg atgataaaca ggaaatccgt cagggtggtt ttgctggctg
    15121 gttaaccgat ggtacaccgc tgtgggttac tggcagtggc accagcaaaa cggtactgac
    15181 ccgttatgcg acggtgctta atcgggtttt gccagtgccg acacaagttg ccagtgggca
    15241 atgtgtagag gtggagctat ttgcgcgcta tccgctgaag aaaatcacag cggaaaagag
    15301 tacgacggca gtaaaacccg gcgtacttaa tgggcgctat cgtgtgacct ttaccaatgg
    15361 taaccacatt acgtttgtca gccacggtga gacgacttta ctgaacgaaa agggcaaact
    15421 gaaattgcag tcgcatctcg atcgtgaaga gtacgttgcc agagttctgg atcgcgaggc
    15481 gaaaagtacg ccacctgagg cagcgaaagc catgaccgtg gcgatacgca cttttttaca
    15541 gcaaaacgcc aatcgggaag gcgattgcct gaccatcccc gacagcagcg ccacgcagcg
    15601 tgtttctgct tcgccagcta ccacgggggc gcgaacgatg accgcctgga cgcaggatct
    15661 catctatgcg ggcgatccgg ttcattatca cggcagtcgc gctaccgaag ggacgctttc
    15721 ctggcggcaa gccatggccc aggcggggca gggagatcgt tacgaccaga tactcgcgtt
    15781 tgcttatccc gacaacagcc ttagccgctg gggagcgcca cgctcaacct gtcagttatt
    15841 acccaaagcg aaagcctggc tggcgaaaaa aatgccgcag tggcggcgta tattacaagg
    15901 cgagacgggg tacaacgaac cagacgtgtt tgcggtctgt cgtttagtct caggtttccc
    15961 ctataccgat cggcagcaga aacggctgtt tattcgtaac ttctttacgc ttcaggatcg
    16021 gctggattta acccatgagt atctgcacct ggcctttgat ggttatccca ccgggcttga
    16081 tgagaactat atcgaaacgc tgacccgaca attattaatg gactaatgct atgcgaaaga
    16141 tttttcttcc gttgttactg gtggcgcttt cgcctgtcgc tcacagtgaa ggtgtgcagg
    16201 aagtcgagat tgacgcaccg ctctcaggct ggcatccggc agagggcgaa gatgccagtt
    16261 tttcacaatc gatcaattac ccggcgtcgt ccgtcaacat ggcagatgat caaaatatct
    16321 cagcgcagat ccacggcaaa ataaagaatt atgctgcggc gggtaaagtt cagcagggcc
    16381 ggctggtggt caacggtgcc agtatgccgc agagaattga atccgatggt tcatttgcac
    16441 gcccttatat tttcactgaa ggcagcaaca gcgtgcaggt catcagcccg gatgggcaaa
    16501 gccgacaaaa aatgcagttt tactcaacgc cgggtacagg aacgattcgt gcacgtttac
    16561 ggctggttct ctcgtgggat acggacaata ccgatctcga tctccatgtt gtcacgcccg
    16621 atggcgaaca cgcctggtac ggtaacacgg tgctgaaaaa cagtggtgca ctggatatgg
    16681 atgtcacgac ggggtacgga cccgagattt tcgccatgcc agcgccagtt cacggacgct
    16741 atcaggtgta tatcaactac tatggtgggc gcagcgaaac ggaattaacc accgcccaac
    16801 tgacgctgat caccgatgaa ggatcggtca atgagaaaca ggaaacattt attgtaccga
    16861 tgcgtaatgc tggcgagctg acgctggtga aaagttttga ctggtgatcc atcccttcac
    16921 acaacctggc tctttctttt ttcagataag actgctgggc gatttgccga cgccaggata
    16981 taaccattaa caatcgtcgg cagattggtc atttcttatt tttcgtactg agaactgtcc
    17041 atttactccg gctttcctgg ttaaggcaat ggtttctaag cgatacgcat tattgcaatg
    17101 gtgacagaac attgcggtaa cacgctttta ccgctacctt aaccacactc catcggtcac
    17161 ctgaggcgga gcttcgcccc tttgaaatac cttgcttctt ttcgtacaac tctgaaagcc
    17221 tcgcgctaca tgttcagagc attggcgtta gtgctctggc tgttgattgc tttttcatcc
    17281 gttttttaca tcgttaatgc gttacatcag cgagaatcgg aaattcgtca ggaatttaat
    17341 ctgagttccg atcaggctca gcgctttatt caacgcacct ctgatgtgat gaaagagctg
    17401 aagtacatcg ctgaaaatcg cttatcggca gaaaacggtg tgctttcccc gcgtggacga
    17461 gaaacgcagg cggatgtgcc tgcgtttgaa ccgctgtttg ccgactctga ttgttccgca
    17521 atgagtaaca cctggcgagg ttctctggag tcattggcgt ggtttatgcg ctactggcgc
    17581 gataattttt ctgcggctta cgatctcaac cgggtatttt taatcggcag cgataacctc
    17641 tgcatggcca atttcggtct gcgtgatatg ccagtggaac gcgataccgc gttgaaagct
    17701 ttgcatgaac gcatcaataa atatcgaaat gcaccacaag atgatagcgg cagtaacctc
    17761 tactggatca gcgaaggtcc gcgccctggc gtcgggtatt tttacgcgtt gacgccagtt
    17821 tatctggcga acaggttgca ggcgcttttg ggtgtcgagc agaccatccg gatggagaat
    17881 tttttcttac cgggtacgtt gccgatgggg gttaccattc ttgatgaaaa tggtcatacc
    17941 ctgatttcgc ttaccggacc agaaagtaaa attaagggcg atcctcgctg gatgcaggaa
    18001 cgctcctggt ttggctatac ggaagggttc cgggagctgg tgctgaagaa aaatctgcca
    18061 ccctcatcgc taagcatcgt gtattcggtg ccggttgata aggtgctgga acgcattcgc
    18121 atgttgatcc ttaacgcaat tttgctgaat gtgcttgccg gagctgcatt gtttactctc
    18181 gcgcggatgt acgagcgacg tattttcatt ccggcggaaa gcgacgccct gcgactggaa
    18241 gaacatgagc agttcaatcg caagattgtc gcctccgcgc cagtgggtat ctgcattttg
    18301 cgtaccgctg atggcgtcaa tattttaagt aacgaactgg cgcataccta tctcaatatg
    18361 cttacgcatg aggaccgcca acgactgaca caaattatct gtgggcagca agtcaatttt
    18421 gttgatgtcc tgaccagcaa caataccaat ctgcaaatca gcttcgtcca ttcgcgctat
    18481 cgtaatgaaa acgtggccat ttgtgtgctg gtggatgttt cttcgcgcgt gaagatggaa
    18541 gagtcgttgc aggagatggc acaagcagcg gaacaggcga gccagtcaaa atcgatgttc
    18601 cttgccaccg tcagtcatga gctgcgaacg ccgctgtatg gcattatcgg taacctggat
    18661 ctgttgcaaa ccaaagagtt accgaaaggc gtcgatcgtc tggtgacggc aatgaacaac
    18721 tcttccagcc tgttgttgaa aattatcagc gatattctcg atttctcgaa gattgaatcg
    18781 gaacagttga agatcgaacc gcgtgagttt tcaccgcgtg aagtgatgaa ccacatcacc
    18841 gccaactatt taccgctggt ggtacgcaag cagttaggct tgtactgctt tattgaaccg
    18901 gatgtgccag tggctttaaa tggcgacccg atgcgtttac agcaggtcat ctccaacctg
    18961 ttgagtaacg ccataaaatt caccgatacc ggctgtatag ttttgcatgt ccgcgctgat
    19021 ggcgattatc tctctatccg tgttcgcgat accggcgtgg ggattccggc gaaagaagtg
    19081 gtgcgcttgt ttgatccctt cttccaggtc ggaacgggcg tacagcgaaa cttccagggg
    19141 accggtctgg gtctggcgat ttgtgaaaaa ctgatcagca tgatggacgg cgatatctcg
    19201 gtagattcag aaccgggaat gggcagccag tttaccgtgc gtattccgtt gtacggcgct
    19261 cagtacccgc agaaaaaagg cgtggaaggg ctgagtggta aacgctgctg gctggcggtc
    19321 cgcaatgcgt cgctctgtca gttcctggaa accagtttgc agcgcagcgg catcgtcgtt
    19381 acaacatacg aagggcagga accgactccc gaagatgtgt taatcactga cgaggtagtg
    19441 agtaaaaaat ggcagggcag agcggtagtg accttctgtc gtcgccatat tggtattccg
    19501 ctggagaaag cgccagggga gtgggtacac agtgtggctg caccgcatga gctaccggca
    19561 ttgttggcgc gtatttattt gatcgagatg gagagcgacg atcctgctaa cgctctgccg
    19621 tcgacggaca aagcggtcag cgataatgac gatatgatga ttctggtcgt ggatgatcat
    19681 ccgattaacc ggcgtttgct ggcagatcag ttgggatcgt tgggctatca atgtaaaacc
    19741 gcgaatgatg gcgtcgatgc gcttaatgta cttagcaaga atcatattga tatcgtgctt
    19801 agcgacgtca acatgcccaa tatggatggt taccgcttga cgcaacgcat tcgtcagttg
    19861 ggactgacgt tgccggtaat cggagtaaca gctaatgcgt tggctgaaga gaagcagcgg
    19921 tgtctggagt ccggtatgga cagctgtctg tcgaagccag taacgctgga tgtgataaaa
    19981 cagacgctga cggtatatgc cgagagggtc aggaaatcgc gggaatcgta ggccggataa
    20041 ggcgtttacg ccgcatccgg cattcagtgc aaatgccaga taagacacta acgcgtctta
    20101 tctggcctac aggtgattag tctttatctg ccggacttaa ggtcacagaa gagagataat
    20161 tcagcagggc gatatcgttc tcgacaccca gcttcatcat cgcagatttc ttctggctac
    20221 tgatggtttt aatactgcgg ttcagctttt tagcgatctc ggtcaccagg aagccttccg
    20281 caaacaggcg cagaacttca ctctcttttg gcgagagacg cttgtcaccg taaccaccag
    20341 cactgatttt ttccaacagg cgagaaacgc tttccggggt aaatttcttc cctttctgca
    20401 gcgcggcgag ggctttcggc agatcggtcg gtgcaccttg tttcagcacg atcccttcga
    20461 tatccagatc caataccgca ctaagaatcg ccgggttgtt gttcatggtc agaacaataa
    20521 tcgacaggct tgggaaatgg cgcttgatgt acttaattaa ggtaatgcca tcgccgtact
    20581 tatcgccagg catggagaga tcggtaatca acacatgcgc atccagtttc ggcaggttgt
    20641 tgatcagtgc tgtagagtct tcaaattcgc cgacaacatt cacccactca atttgctcaa
    20701 gtgatttgcg aataccgaac aagactatcg gatggtcatc ggcaataatt acgttcatat
    20761 tgttcatgta cttgcaccta tgtattaact atttgatttt aaaaatatat tctatgcttt
    20821 gtttagtcgt tggggcacct gtgggacata agtacatagc tttctgttta gtacttctac
    20881 ctgggactgg ttattgtctt tcatccatgc accgtaaaca ttgtaaacca tctgggcatt
    20941 cgcatgtccc atctggttgg ctatgaagtt tggatttgct ccggcagtta aagcccagca
    21001 agcataagta tgccgagact gatacgcttt tctatggcga atttttgctc gttttaatgc
    21061 agattcccat gttctgttga tggatgttac ggcataatga actcctgagt tacctgtgcg
    21121 atctaatatc tgaggattga aaacaaagct gcatgagtga gtgcttgtcc ggccatactc
    21181 tcgcagcttt acttctatct gatattgttt accaagcctg gttaatgtcg cctgattttt
    21241 taatacgtca attgctggct gtatgagttg tattaccctg tttgttcctg catctgtttt
    21301 gggtagtgta aattcctttg tctgagtgta attccttttg acggtcaagg tacccgcttc
    21361 cagatcgata tcctcccatg ccagcccaca cagttcgcca tgccgcatcc cggtatatac
    21421 agccagcgac cagaaatttt tagtttgctg gtggtggcac gcatcaatca gtctggcaaa
    21481 ctcatctttt gttagtggat cgggaggtgt tttcgctctt ttcagtgatg gaagctcact
    21541 gaaagggttt ttgcctatat acccattgtt tgccgcaaaa cggaacatcc cggagataat
    21601 tgtcatatag ctatttacag taggtacact acgaccttta ataggcttcc tctgattttt
    21661 tcctggcaac tgatatcccg ttaataattc tttacgaaaa atgagaacat cttcctgtgt
    21721 aattgatgat gccagtttgt tccctccaag gcagtcaagg caatttcgga tagctgattt
    21781 gtatctgacc atggcatttg ctgttatttc cattttctta agctcaagcc atttttccga
    21841 aagtgcctta atggttatct ctttttttcc cagaccaaag tgtttcaggt taggggaatt
    21901 agggaactgc gcggcgtagt cgaagctccc cattctgatt gcaaaacaaa cggaagtacg
    21961 aagttcacct gcgatcttcc ggtttttggc ggtgtcagga acaccgaggt tttctctgac
    22021 acgtttgccg ttatagtgaa accatatgcg gagtgatccg ccatggtttt caacgcctgt
    22081 cgggtatgat gcgttactca ttaaacctcc cagacgtcca ggagcattaa caggttaacc
    22141 ggaacttgca tttttggcac ctggttgttt ctggttttcg atccagcgca taatttcctc
    22201 aatgttgtat acacattcac tgaaaggccc gggctctcct tctgctgcgt aatgacggta
    22261 ttctttcccc agtaaccagg atttttttct tgctctggca atggttcctg atttcagtcc
    22321 agttgcagca ataagggttc tttctgtgca ccatttgctg ggcgttatct gatagatgat
    22381 tgtttccatt cctgactcac actatcttca ggccacggca gtggcaccac acgtcaaaca
    22441 ttcgctttac aacttcacgg cagtagaagc cgtgagcatc tctggttaaa tcgtatcggt
    22501 acccgtatct gagtcgtatc catatttcaa actcgcgatt cacgacttat tctcctgctg
    22561 tctttatgcc agttcatccg gttgcgctgg ggcccaggcc ttccgtggcc tgtatttgcg
    22621 gcgttttctg gcttcaatgc gtatctttgt caccctgact ggctttggtt cgtgccggaa
    22681 cactttgttt ctgatattac gccgtgtatt catcatccag ataaaacgct gggtatggtc
    22741 gcatccgtca tcaacggtaa ttactgtgcg gaccagttca tcgttaatat caaccatttc
    22801 acccattcac tgagccctca tacgcgaatt tcgtggcttc catcagttgc gtccataccc
    22861 attcaagaaa ttgtgcgttt tctcctgagc ggtcatattc atcgacgctg cggggcttga
    22921 tggtcgccag tagtggcaca atccggtact tcatctgttc ggttaacttg tccggtacaa
    22981 gtcgccagcc gttatatttc gatggaacat ggtggtattc agccaggaag cgtcgtatat
    23041 cgtttttcat ttcctcgcat gagttctgat catgaagtgc agcggaaagc cagcgtccga
    23101 tatattcagg gattcgcaca tcaagtactt tttctgccgt cgtcagcatt gcctgtcggc
    23161 aggcgttcca gccgttgaca tagtgacgtg ttgcatccca tgaaacctgc acatcagggt
    23221 aagcatcctg ttctgttctc actggcggta tctgaggaac agtgaatagt ggactgacga
    23281 tatatttatc cagagagcct ccccgctctt ctgcaagact ttcccacgat gtcattgtgg
    23341 ggaccctgta acctgctttt ttgtgcgtat acatccatgc cactctgccg ttatcatctg
    23401 cggcggcaat gcgctgccag gcaggataaa gcggggtgta tgtttctctg tcattacggg
    23461 cgtcaggcat ggcatcaata ctgcaaactt cccccgtaag gttattcagc caggccttcg
    23521 ggctaatctc tgacgttacg atttcatcca gttttttctc cagattttct atgtgactga
    23581 ttagcgcaag gataactggc ggagtggcaa gggagtgaaa tgcttccaga tcacatccct
    23641 tgtctccata tacggtattt tgtgcggcgt cacgcagtgc tttcagatca gcttctttca
    23701 tcgttcagac tcccttgttg ctgttttgta ggcgcgcagg atgtcgcgtg tttttcctga
    23761 taatgacgac ttaaccagga aaaagccgct ccggctttct gtgatgtccg gcgtgcacag
    23821 aagtaccgca tcaacgacac gattatgttt tcgcaatgtc aggcgggaac tggtgataat
    23881 cagttttgcc tgttcgccgt aatcggtgaa tgaaataatc atctgatggc gatctccgtt
    23941 atgaaagtgt tcctgctggt tcaatgctga taatttctgc tgcaataagc atacctgcca
    24001 gccatgcttc gccgagcatg tcctcatccg tacatgcaag atcaccgatc tgtattgtgg
    24061 ccataatgtt agtttcaccg gtatattcat cctgtgcttc ttcatacggc aggttttcat
    24121 aaaggttttc aattgcgcag gttattacat ccagtcccgt cagattgccg acaacgtcca
    24181 cttcaaatgt ttcgcggtac tcccattgcc cgagtgtcag acgaaccgtc tgtcttgcca
    24241 tgcgcccgca taatgtcatg ttcgggtcgt agttcatgat tgccgcagta gtgctattca
    24301 tgttctgcct ccggtgtgta aatagctttg tcgtgtttaa attcaccgtt ccagtttttc
    24361 ttcataggga gttcaccgtt catgtaaaac tggtataaac ggtggcatcc tttttccagc
    24421 aatacggggg tgaatttcgt gaatgttgct ttcccgtgtg gagtgatctg tactgcttct
    24481 tcggtcaggt atctgtcacg ggcataagag gccgtgcgcc agcggggtgt tttctccgga
    24541 tcgcgttgtt cgttgaatac ccattttcgg ccagacagcc accacattat ctggctggta
    24601 tttacgccgt tcagcgcacg gcagaactgg ggaacagtca tgcctttaga gaaatgtttt
    24661 tccagactgt ctacggttgc agagagcgtt ttattttcca gggccatagc ttcagcccgt
    24721 tcttcggcct caataaccat caacgcgagt tccttgcgag tgagtacgcg ctgaacatcg
    24781 atgttttccc gctgagtgaa gtagaactcc acaaggtctt cgtgatagtt ccacgcctga
    24841 tccgtttcca gcaattttgc gtggttggct gcgccgcgtt cggtccagag gatgagggat
    24901 cgaacattgc tggcaatttt cacagagtag ctttgagata ctctgtgctt aagttcacgt
    24961 agttcatcgc cttccagcaa aaaataatgc tttccaacaa cgaatcgttc ttcgtttcgg
    25021 gaatgattct ggcgaatacg gattggtgtt gctccataac ctgccgccag ttgttcagtg
    25081 gtcacaacgc gttgaccgcg atattcgata atttgcaaat cacgggctgc caccggcaca
    25141 agttcagttg attttttcat cattacacct cacgaaaagc tgattagtgg aacactctgg
    25201 tttccggttc tgagttgtca gtggcttcac tgataagccc atccagaatc atgtgcatcg
    25261 ctctgtaacc ttccattgtg atgccggttt tttcgccgtc gcccagagcg ccggtgaatg
    25321 ctttgtacat aagaatgctt ttgagcagcc ctgattccgc tccgtaagtt tctatgagtg
    25381 cccattccat atagttctgc atagcgagac gtaatggttt caggtagatt gtcatgccac
    25441 cgttgtggcc tctgtacatg gtgcccttgt ctgtaccgcc attctcgtta agcacttcgg
    25501 tccagccgtt tctggtgagc tgttcagaga agaacatggc ggtataaagc cagcgccagt
    25561 acatgattgt cttttccggc gctagctcac ctgtcatgcc tttttcttct gcttcactga
    25621 tgcaacacac tatttccaga ccatgccaga tcggctcgtc aaaacagcct tcatcgagca
    25681 tttccagtgc tttgttgata tcaagcagtc cgtaaggtgt gtggatacct tcagcggtaa
    25741 gaaaaaataa ctgttcagtt gcagtcatgg tttgctctct tcttaaatgg tctttgcgcg
    25801 acaagccggc aatactgatc aatccaggcg ttgtattttt ccatccaggc atcaaactgt
    25861 tttctgcgtg caaggatgcg caacaggcga tgaaaacaac gcccgtgagc tctgtagtat
    25921 tcgggcgtaa tttctcccgg atgaaagact tctccatatt ccggatgcat caggcgctgg
    25981 tcaggcagac gtttttcaaa acctgaactt tcgaatacct tcgttgtcat aaaatgagcg
    26041 agatgtatga tggccgtttt tcgcgtgaag catttcttaa cttgtccgtg gcggcagact
    26101 ctgtaaacag ggccgcaatc gagttcgtat ttatgaaaag cacggtcgat tccggcatta
    26161 tttattgcac tgtattccgc atcactgaag aaaggtggaa atacgatgta tccttccatt
    26221 gtcagcgcca gaacgcgcgg atcggtttct gtcagccatt cgttttctgt gaattcttca
    26281 gaattaattt caatgatttc aggttcttct ttcatggtta ctccgtgttc atccggtgtt
    26341 aattactttt attcactctg agcgcagcca gaagatgtgg ctcagcaatt cgtttgttgc
    26401 gatatatctg tgatacagcc tggccttttc aggtcgtaaa aatccccgac aataaagctg
    26461 taaattaatt actgttctta aataatgtta ttcgttttca gtccggcaca gatgcttttc
    26521 tgctttgtct gctgtacagg ataatgagcg gaaaagacaa ttcatcatgt ttagcgcatc
    26581 tggtgaacca atatcaccgc tgtcaataat cacctgtaac aaagacaggc tttctgttat
    26641 ctcgcagtgg acattatcga gtacttcaac gcaattattc atttttcatt atctccgtta
    26701 ttccggggga ggagctatgc tgttaatgat taacgcatcc ggtaatatgc ccctctttaa
    26761 tgtaatcgat gcattcattc aaaagttcat caatgattaa ctttcctgat tcagtcagat
    26821 actcaccatg ttggttgata ccgacagcat cctggtatgc agtgcgtatt gctgtttcac
    26881 catctttccg cccaaactca ccacgggtaa cgccttcaaa tcgtaatagc aactggctta
    26941 tgaactgttc tgttatttca atagttgtta actctccatc atgacgcttt acagtgagga
    27001 gattacttcc tgtttttcgt ttcatccgcc gcagtgctgc aaccgttatg cggcgacgat
    27061 atgcagaaat gtattcatct ttcatttgtt atttcccgta tgcctttttg agaaacaata
    27121 ctgcaactga ccagtatccg gcatcagcca ttaataaagc ggttttatag gcatgtttgt
    27181 ttttcatgtg tcatccccat tttaaaatag tgagaactcc cgaccgcaga ccgtcatgtt
    27241 tttatgtatg attgtctgtt taatttcctt tattcgttct cagagcatca acatactcgt
    27301 aagccttttc acaggtttta ttcatggaac gaatcagaca aagtaagaaa tcatctgtct
    27361 ctccgttatc accagagttt ttaaatataa actcaagcat tgacgtgttt tcttttattt
    27421 ctgctgccac ttcctcaagc ataatcaggg gagttttcat gttctttgtt ccttaaatgc
    27481 atcgcatgcg cttctggcgt attgttgtgc cagtaaaaat atgtcatccg aaagttcatc
    27541 acattcttca tcaccggaag ccgaaatgat taaccccgct tcaagcagta ctgcaatgtg
    27601 atgaaaagct gtttccggtt cgttggtgag gcctttgaac attttcatct tacgattcct
    27661 ttgtcgtgtc agaatggaaa cttgtaagta accacatcgg atcgcagtca agtatatttg
    27721 ccagaggaat cacctggctt gccagaggtt cagccttgcc gttctcccac tggatgatgg
    27781 tttcttcatc aactccaagc agcgtggcga gttcagcggt ggatagaccg cagctttcgc
    27841 gttgggtgcg gatgccatca cggcaatctt ttttgtttct cggaggtaac agaatatgtt
    27901 ttagttgttg gattttttcg attccaacct caaggagtac gtgacggttt ctttccgata
    27961 cagactccag actttcatga agcagcgatt ctacttgttc gataagcatc aattgtgaag
    28021 tagacataaa aaaacctcgt aaccaatatt tcgaaatcta ggttacgagt gagtaatgta
    28081 ggtgtcaaga gcaattaatg ttttttgttg aattaaattt gcatacgcat ctcaacgaca
    28141 acaccaataa tttcacaatc cccattgatg gggatcagtg gatagcgtgg gtttagtggc
    28201 tttaggaaac attgcccagc gtcttctatg tactgtttaa gggttgcctc tccggagttt
    28261 ttgagcctgg cgacgacata ttttcctgaa aaaacttctt tgtcagggtt aacaagaatg
    28321 ttcatgcctt caaaaataga gaactctcca ttctgtgatg tcattgagtc ccctttcact
    28381 tccagccaga agccgtgatc gcccgcatat atatcgctac caatccattc ttctggataa
    28441 gctatgtaat cttcgcagtt ccaggggcca gcactcaccc atgttagttt cggatatcta
    28501 taacggtttg gagaaactgg cttaggattt cggacgtttt gaatgctatc tgtaggtgat
    28561 ggggcgctgc cgtcaagaag ccatttagga gagcatttca atgcatccgc gattctaaat
    28621 aggtttgaag atcttgtgtc ttgtgagtct cctaactcta ttttgctgat tgttacacgc
    28681 gagacaccag tcaattttgc caggtcatct tgtgacattc ctagctgtac tctgcgcatt
    28741 aacagtctgc caccaaagct atcgtgatcc atcttctccc ccttcttgtc gaaataatcg
    28801 tttcgaaaca aatatagcaa aaaatcttga aatctaggtt tcggaaaatt aagattaatc
    28861 gaaacaaacg tgtcgaggtg gatatggatt tgtatgaaat tctaaaaaac atgtttggat
    28921 cgaatgttga aataggtcgt tattttcccc gaaggggaag ggctagaacc ggacaggctg
    28981 taggtaagtg gaaaacccga ggtgtgccgg aagacgtggc gatcctttgt catctggatc
    29041 ccaaaattcc ttatcagcat ccgtcgctca tgaatgccag tcatgaatcg tgatgttctg
    29101 ccggaggctg tatgtcccct gattacgttc agatggagat gcctgccagg tacagccagg
    29161 cagattcaga gtggattaag cggcagttac agagcctgcc ttcctcactg agacagaagg
    29221 ttgccctgaa atacgcggag gtttacgaag ttacttttga ttccgagcct gtttcattcc
    29281 gtaaggagaa cagagcgagg catgaagcca atgtacggct gcgcaggttc gttgaaacac
    29341 acgggcgtgc attacagggg tatacgactc agccgcccct ggcaggaacg caacagcgta
    29401 cctgaatggt gttggtctta aaggtgaccg acgggctgag accccacttc tcaggatgca
    29461 cctgtgtact acc
//
LOCUS       DAZFKF010000057        28982 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFKF010000057 DAZFKF010000000
VERSION     DAZFKF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.057"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            313..1179
                     /gene="folD"
                     /locus_tag="ACHKA1_003002"
     CDS             313..1179
                     /gene="folD"
                     /locus_tag="ACHKA1_003002"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO5680622.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1181..1393
                     /gene="ybcJ"
                     /locus_tag="ACHKA1_003003"
     CDS             1181..1393
                     /gene="ybcJ"
                     /locus_tag="ACHKA1_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO5680623.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1501..2022
                     /gene="ybcI"
                     /locus_tag="ACHKA1_003004"
     CDS             1501..2022
                     /gene="ybcI"
                     /locus_tag="ACHKA1_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5680624.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(2058..3443)
                     /gene="cysS"
                     /locus_tag="ACHKA1_003005"
     CDS             complement(2058..3443)
                     /gene="cysS"
                     /locus_tag="ACHKA1_003005"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO5680625.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3617..4111
                     /gene="ppiB"
                     /locus_tag="ACHKA1_003006"
     CDS             3617..4111
                     /gene="ppiB"
                     /locus_tag="ACHKA1_003006"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO5680626.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4114..4836
                     /gene="lpxH"
                     /locus_tag="ACHKA1_003007"
     CDS             4114..4836
                     /gene="lpxH"
                     /locus_tag="ACHKA1_003007"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO5680627.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARNADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4954..5463
                     /gene="purE"
                     /locus_tag="ACHKA1_003008"
     CDS             4954..5463
                     /gene="purE"
                     /locus_tag="ACHKA1_003008"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO5680628.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5460..6527
                     /gene="purK"
                     /locus_tag="ACHKA1_003009"
     CDS             5460..6527
                     /gene="purK"
                     /locus_tag="ACHKA1_003009"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO5680629.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(6666..7559)
                     /gene="ybcF"
                     /locus_tag="ACHKA1_003010"
     CDS             complement(6666..7559)
                     /gene="ybcF"
                     /locus_tag="ACHKA1_003010"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO5680630.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7556..8371)
                     /gene="ylbF"
                     /locus_tag="ACHKA1_003011"
     CDS             complement(7556..8371)
                     /gene="ylbF"
                     /locus_tag="ACHKA1_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO5680631.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVTQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8382..9641)
                     /gene="ylbE"
                     /locus_tag="ACHKA1_003012"
     CDS             complement(8382..9641)
                     /gene="ylbE"
                     /locus_tag="ACHKA1_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO5680632.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNQGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9651..11318)
                     /gene="fdrA"
                     /locus_tag="ACHKA1_003013"
     CDS             complement(9651..11318)
                     /gene="fdrA"
                     /locus_tag="ACHKA1_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO5680633.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFRHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGAGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALCLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGNLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11635..12684
                     /gene="allD"
                     /locus_tag="ACHKA1_003014"
     CDS             11635..12684
                     /gene="allD"
                     /locus_tag="ACHKA1_003014"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO5680634.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12706..13941
                     /gene="allC"
                     /locus_tag="ACHKA1_003015"
     CDS             12706..13941
                     /gene="allC"
                     /locus_tag="ACHKA1_003015"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO5680635.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLETQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPHVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            13952..14737
                     /gene="allE"
                     /locus_tag="ACHKA1_003016"
     CDS             13952..14737
                     /gene="allE"
                     /locus_tag="ACHKA1_003016"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO5680636.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14866..16011)
                     /gene="glxK"
                     /locus_tag="ACHKA1_003017"
     CDS             complement(14866..16011)
                     /gene="glxK"
                     /locus_tag="ACHKA1_003017"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO5680637.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEACGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNDIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(16033..17334)
                     /gene="ybbY"
                     /locus_tag="ACHKA1_003018"
     CDS             complement(16033..17334)
                     /gene="ybbY"
                     /locus_tag="ACHKA1_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO5680638.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(17391..18752)
                     /gene="allB"
                     /locus_tag="ACHKA1_003019"
     CDS             complement(17391..18752)
                     /gene="allB"
                     /locus_tag="ACHKA1_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO5680639.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(18812..20266)
                     /gene="ybbW"
                     /locus_tag="ACHKA1_003020"
     CDS             complement(18812..20266)
                     /gene="ybbW"
                     /locus_tag="ACHKA1_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO5680640.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(20435..21313)
                     /gene="glxR"
                     /locus_tag="ACHKA1_003021"
     CDS             complement(20435..21313)
                     /gene="glxR"
                     /locus_tag="ACHKA1_003021"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO5680641.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     NVDTARQVTEAADIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21414..22190)
                     /gene="hyi"
                     /locus_tag="ACHKA1_003022"
     CDS             complement(21414..22190)
                     /gene="hyi"
                     /locus_tag="ACHKA1_003022"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO5680642.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(22203..23984)
                     /gene="gcl"
                     /locus_tag="ACHKA1_003023"
     CDS             complement(22203..23984)
                     /gene="gcl"
                     /locus_tag="ACHKA1_003023"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO5680643.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(24074..24889)
                     /gene="allR"
                     /locus_tag="ACHKA1_003024"
     CDS             complement(24074..24889)
                     /gene="allR"
                     /locus_tag="ACHKA1_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO5680644.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNSNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(24967..25449)
                     /gene="allA"
                     /locus_tag="ACHKA1_003025"
     CDS             complement(24967..25449)
                     /gene="allA"
                     /locus_tag="ACHKA1_003025"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO5680645.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25679..26605
                     /gene="allS"
                     /locus_tag="ACHKA1_003026"
     CDS             25679..26605
                     /gene="allS"
                     /locus_tag="ACHKA1_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO5680646.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26674..27768
                     /gene="mnmH"
                     /locus_tag="ACHKA1_003027"
     CDS             26674..27768
                     /gene="mnmH"
                     /locus_tag="ACHKA1_003027"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO5680647.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHAAGIDYPLVAGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            27884..28249
                     /gene="ylbG"
                     /locus_tag="ACHKA1_003028"
                     /pseudo
     CDS             27884..28249
                     /gene="ylbG"
                     /locus_tag="ACHKA1_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(28770..>28982)
                     /locus_tag="ACHKA1_003029"
     CDS             complement(28770..>28982)
                     /locus_tag="ACHKA1_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680648.1"
                     /translation="RFKKTLTVRAREQVAKIVSKNDPDTKKVWCKYGKIPGQGDSVNL
                     FFVGEINVTHYFITNIGAGLPDACAE"
BASE COUNT         7142 a         7834 c         7207 g         6799 t
ORIGIN      
        1 cgcaaaatat attcaacaca aatatagacc tgaagcggta aattaccagg ctgaaaattc
       61 tttttatatt gtcaggtatt tcttaaatta tcttaatcct ttgacaagga aataagtcag
      121 ttccagattt acaacgccat catggacgaa aaatgaagct ttcaatctca gcgacctcag
      181 cgacggtgcg cctaaccttc gcaagaggcg cttcacgcga taaatctgaa acgaaacctg
      241 acagcgcgcc ccgcttctga caaaataggc gcatcccctt cgacctacgt aacagatgga
      301 atcctctctc tgatggcagc aaagattatt gacggtaaaa cgattgcgca gcaggtgcgc
      361 tctgaagttg ctcaaaaagt tcaggcgcgt attgcagccg gactgcgggc accaggactg
      421 gccgttgtgc tggtgggtag taaccctgca tcgcaaattt atgtcgcaag caaacgcaag
      481 gcttgtgaag aagtcgggtt cgtctcccgc tcttatgacc tcccggaaac caccagcgaa
      541 gcggagctgt tggagcttat cgatgcgctg aatgccgaca acaccatcga tggcattctg
      601 gttcaactgc cgttaccggc gggtattgat aacgtcaaag tactggaacg tatacacccg
      661 gacaaagacg tagacggttt ccatccttat aacgtcggtc gtctgtgcca gcgtgcgccg
      721 cgtctgcgtc cctgcacccc gcgcggtatt gtcacgctgc ttgagcgtta caacattgat
      781 accttcggcc tcaacgccgt ggtgattggc gcatcgaata tcgttggccg cccgatgagc
      841 atggaactgc tactggcagg ttgcaccacc accgttaccc accgtttcac taaaaatctg
      901 cgtcaccacg tagaaaatgc cgatctactg atcgttgccg tcggcaagcc gggctttatt
      961 cctggtgact ggatcaaaga aggcgcaatt gtgattgatg tcggcatcaa ccgtctggaa
     1021 aatggcaaag ttgtgggcga cgtcgtgttt gaagacgcgg ctaaacgcgc ctcatacatt
     1081 acgcctgttc cgggcggcgt tggcccgatg acggttgcca cgctgattga aaacacgcta
     1141 caggcgtgcg ttgaatatca tgatccacag ggtgagtaac atggcgacat tttctttagg
     1201 taaacatccg cacgttgagc tgtgcgactt gctgaaactg gaaggctgga gcgaaagcgg
     1261 cgcgcaggcg aaaatcgcga ttgccgaagg ccaggtgaaa gtcgacggcg cggttgaaac
     1321 gcgcaaacgc tgcaaaatcg tcgccgggca gacagtgagt tttgcaggtc acagcgtaca
     1381 ggttgttgcc tgatcccttt cccggcctgt atcaacaggc cggttctctt ttcagacttt
     1441 tgccctctac gccccgttca tccatgctga tagcatcgac attttgccga agcgaccatc
     1501 atgccaaccg ttattacgca cgccgccgtt cccctatgca ttggtttagg gttgggttcg
     1561 aaagtcatcc ctccgcgtct gttatttgcc ggaatcatcc tggcgatgct gcccgatgcc
     1621 gacgtcttgt cgtttaaatt tggcgttgct tacggcaatg tttttggtca tcgcgggttt
     1681 acccattcgc tggtgtttgc gtttgttgtc ccgctgttat gtgtgctgat tggacgtcga
     1741 tggttcaggg ctgggctgat tcgctgctgg ctgtttttaa ccgtctcgtt gctatcgcac
     1801 agcttgctgg attcggtaac cactggcggt aaaggggttg gctggctgtg gccgtggtca
     1861 gatgaacgct ttttcgctcc ctggcaggtg attaaagtcg cgccgtttgc attgtctcgt
     1921 tacaccacag cgtacgggca tcaggtgatt atttctgaat tgatgtgggt gtggttgccg
     1981 gggatgctac tgatgggaat gttgtggtgg cgcagacgat aaccggatgc gaaaactcgc
     2041 atccggcaat agcgcaatta cttacgacgc caggtggtcc cttgcgggcc atcttccagc
     2101 acgatcccca tctcgttaag acggtcacgc gccgcatctg ccgccgccca gtctttcgct
     2161 ttacgggcat ccagacgctg ttgaattaac gcttcaatct cagccacttc gctgtcatct
     2221 gcctgcgcgc cgctttgcag gaacgcttcc ggttcttgct ccagcaggcc caatacagcg
     2281 gaaagtttac gcaggtgaga tgccattgca ttcgccgctg ccatatcttc tgctttcaga
     2341 cggttcactt cacgcgccat atcaaacagc acggaatagg cttccggggt gttgaaatcg
     2401 tcgtccatcg cttcaataaa gcgcgcttca aacgcttcgc caccggcagg cgcaacggtt
     2461 ttatctgtgc cgcgcagcgc agtgtagaga cgctccagcg ccgcacgcgc ctgcttcagg
     2521 ttctcttcgc tatagttcag ctggctgcga tagtggcccg acatcaggaa gtaacgcacg
     2581 gtttccgcgt cgtagtattt cagcacatcg cgcacggtaa agaagttacc cagcgatttg
     2641 gacatcttct cgcggtcaac catcaccatc cccgagtgca tccagtagtt cacatactgg
     2701 ccatcatggg cacaggtgga ctgcgcgatt tcgttttcgt ggtgtgggaa catcaggtca
     2761 gaaccgccgc cgtggatatc aaagtggtta cccagctgct tgcagttcat tgccgaacat
     2821 tcaatgtgcc agccaggacg gcccgcgccc cacggagacg gccagctcgg ttcgccctct
     2881 ttcgacatct tccacagaac gaagtccatc gggttgcgtt tgtcgtcgac cacgtcaacg
     2941 cgcgcgcctg cctgcagctg gtcgagatcc tgacgcgaca gcacgccata agttggatcg
     3001 gtcgggacgt cgaacatcac gtcgccgttg tccgccacat aagcgtgacc tttggcgatc
     3061 agttgttcag tgagttcaat aatttctgcg atatggtgcg tcgcgcgcgg ctccatatcc
     3121 gggcgcagaa tgttcaacgc atcaaaatct ttgtgcattt cggcgatcat gcgatccacc
     3181 agcgccacaa agctttcgcc attttcattg gcgcgtttga tgattttgtc gtcgatatcg
     3241 gtaatgttgc gcacatactt cagcttatag ccgaggaaac gcagatagcg cgcaaccacg
     3301 tcaaaagcaa caaaggtacg cccgtgaccg atatgacaga gatcgtaaac ggtgattcca
     3361 cacacgtaca tgccgacttc cccggcgtga ataggcttaa attcctcttt ttggcgtgtc
     3421 agagtattga agatttttag catcgaagat tccgtttaga catgtgtggg taattgagtt
     3481 gcgtataata cccatatttc ccgcgcgaat cagcatacat tgcgtgatga tccgatcgtg
     3541 cggttatgct ataacaccac cctatatatg acccgaactg ggttgaagca ccaatcaaac
     3601 ggaacaggat gcaaaaatgg ttactttcca caccaatcac ggcgatattg tcatcaaaac
     3661 ttttgacgat aaagcacctg aaacagttaa aaacttcctg gactactgcc gcgaaggttt
     3721 ttacaacaac accattttcc accgtgttat caacggcttt atgattcagg gcggtggttt
     3781 tgaaccgggc atgaaacaaa aagccaccaa agaaccgatc aaaaacgaag ccaacaacgg
     3841 tctgaaaaat acccgtggta cgctggcaat ggcccgtact caggctccgc actctgcaac
     3901 tgcacagttc ttcatcaacg tggttgataa cgacttcctg aacttctctg gcgaaagcct
     3961 gcaaggttgg ggctactgcg tgtttgctga agtggttgaa ggcatggacg tggtagacaa
     4021 aatcaaaggt gttgcaaccg gtcgtagcgg tatgcaccag gacgtgccaa aagaagacgt
     4081 tatcattgaa agcgtgaccg ttagcgagta atcgtggcga cactctttat tgcagatctt
     4141 catctctgcg tggaagaacc ggcgatcacc gccggttttc tgcgtttttt agcgggggaa
     4201 gcccgcaatg ccgacgcgct gtatattctt ggcgatctgt ttgaagcgtg gattggcgac
     4261 gacgatccca acccactcca tcgccagatg gcggcagcga tcaaagcggt gtccgattcc
     4321 ggcgttccct gttatttcat tcatggcaac cgtgattttc tgctcggcaa acgctttgcc
     4381 cgtgaaagcg gcatgacgtt attgccggaa gaaaaggtgc tcgaacttta tggtcgtcgg
     4441 gtgttgatta tgcatggcga cacgctgtgc accgatgacg cgggttatca ggcttttcgc
     4501 gccaaagtcc acaaaccctg gctgcaaacg ctattcctcg ccctgccgtt gtttgtgcgc
     4561 aaacgcattg ccgcgcgaat gcgcgcgaac agtaaagaag ccaacagcag taaatcgctg
     4621 gcgatcatgg acgttaacca aaacgcggtg gtcagtgcga tggaaaaaca tcaggtgcaa
     4681 tggctgatcc acgggcatac ccatcgcccg gcggtacatg aacttatcgc caaccagcaa
     4741 cccgcttttc gcgtggtact cggtgcctgg catacggaag gttcgatggt gaaagtcacg
     4801 gcggatgacg ttgagctgat tcattttccg ttttaaaaaa cccgcaactt tgctgatttc
     4861 acagccacgc aaccgttttc cttgctctct ttccgtgcta ttctctgtgc cctctaaagc
     4921 cgagagttgt gcaccacagg agttttaaga cgcatgtctt cccgcaataa tccggcgcgt
     4981 gtcgccatcg tgatggggtc caaaagcgac tgggctacca tgcagttcgc cgccgaaatc
     5041 ttcgaaatcc tgaatgtccc gcaccacgtt gaagtggttt ctgctcaccg cacccccgat
     5101 aaactattca gcttcgccga aagtgccgaa gagaacggtt atcaggtgat tattgcgggc
     5161 gcaggcggcg cagcgcacct gccaggcatg attgccgcca aaacgctggt gccagtgctg
     5221 ggcgtgccag tacagagcgc cgcactgagc ggtgtcgata gcctctactc catcgtacaa
     5281 atgccgcgcg gcattccggt gggtacgctg gcgattggta aagctggcgc ggcaaacgcg
     5341 gcgttactgg cagcacaaat tcttgcgact catgataaag aactgcacca gcgtctgaat
     5401 gactggcgca aagcccagac cgacgaagtg ctggaaaacc cggacccgcg aggtgcggca
     5461 tgaaacaggt ttgcgtcctc gggaacgggc agttaggccg tatgctgcgt caggcaggcg
     5521 aaccgttagg cattgctgtc tggccagtcg ggctggacgc tgaaccggcg gcggtgcctt
     5581 ttcaacaaag cgtgattacc gctgagatcg aacgctggcc ggaaaccgca ttaacccgcg
     5641 agctggcgcg ccatcctgcc tttgtgaacc gcgatgtgtt cccgattatt gctgaccgtc
     5701 tgactcagaa gcagcttttc gataagctcc acctgccgac cgcaccgtgg cagttacttg
     5761 ccgatcgcag cgagtggcct gcggtgtttg atcgtttagg tgagctggcg attgttaagc
     5821 gtcgcactgg tggctatgac ggacgcggtc aatggcgttt acgcgccgat gaaaccgagc
     5881 agttaccggc tgaatgctac ggcgaatgta ttgtcgagca gggcataaac ttctctggtg
     5941 aagtgtcgct ggttggcgcg cgcggctttg atggcagcac cgtgttttat ccgctgacgc
     6001 ataacctgca tcaggacggt attttgcgca ccagcgtcgc tttcccgcag gccaatgcac
     6061 agcagcaagc gcaagccgaa gagatgctgt cggcgattat gcaggagctg ggctatgtgg
     6121 gcgtgatggc gatggagtgt tttgtcaccc cgcaaggtct gctgatcaac gaactggctc
     6181 cgcgtgtgca taacagcggt cactggacac aaaacggtgc cagcatcagc cagtttgagc
     6241 tgcatctgcg ggcgattacc gatctgccgt taccgcaacc agtggtgaat aatccgtcgg
     6301 tgatgatcaa tctgattggt agcgatgtga attatgactg gctgaaactg ccgctggtgc
     6361 atctgcactg gtacgacaaa gaagtccgtc cgggccgtaa agtggggcat ctgaatttga
     6421 ccgacagcga cacatcgcgt ctgtccgcga cgctggaagc cttaatcccg ctgctgccgc
     6481 cggagtatgc cagcggcgtg atgtgggcac agagtaagtt cagttaacct ggtgctcatt
     6541 tgattgccgg atgcggcgtg aacgccttat ccggcctaca ccgctccggt acccattgta
     6601 ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc tggatgctct
     6661 catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc aattcgcgat
     6721 aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccactcac cgccgttacc
     6781 ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg tgtggcatgg
     6841 cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc atcggtgagg
     6901 atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcagcgag atctttatca
     6961 atcaccgctt cactccctgc cccgtcttcc gtcacaggca caccgccgcc gccactgcaa
     7021 atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag aattttacgc
     7081 ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat ctgccagccg
     7141 taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat aaatttctct
     7201 ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac cgtcgtgaca
     7261 ggcggcatct gcggctgtgc gctcaaactc tgcgccagca tatagccaat catcccctgg
     7321 ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc cagattctgt
     7381 aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg ataagaacgg
     7441 gccaggcgcg ccagcgcggg tacagcactg gcgatattgc gatattgatt ttctgccgtc
     7501 agcgcctcac cgcgctgaag caaggcattg cccccaagag ctacaaccag tgttttcatg
     7561 gttttccttg taataattgt tgaccaagcc agaaccccag cagtgtatct gcccctgacg
     7621 tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg caactcagag
     7681 catgaacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa cgtaaataac
     7741 tgacgctcac cgctgtggta acgagttgca gattgtcgga acaggcaaaa aacggacgac
     7801 cggagcgcgc atctaaagcg ccataatacc aggccgccag cagcattccg ctcaacgtgt
     7861 catcatgact tggcgttaat ccggggcctt tacccagcca gtgccgccag tcggtcttaa
     7921 cgccattgag cgcggcctga aaacagtgac gaaactggcg taattcagcg ggcagcggat
     7981 cgcttgccgc catcgccagt ggcccgaaaa gtccggtttc ctccgcgcgt tgcatccatg
     8041 cagctgcaag cggttgagga tgcgaaggcg gcgtaatacg cagcaaacaa taacgctgtg
     8101 gctgtgtaac cgtgaaacgc ccgaggcgaa tcccttgagc cacaacctgt ggtcgttcat
     8161 tgccgcataa tccaccgcat aacgcatcga attgcgcacg gcgaagcacc catcctccgg
     8221 ggccgaaacc actaccctga cgatgcagcg ttaacagttc gccgctttcc gtcatcaggt
     8281 taatcgcccg ccgccacact cccgctaacc gccaggactg gcgataattc ggtgcgctac
     8341 tactggcaag cagaggatgg atgatcgtca ttgcgcgttc ctcaaccaat acccatgctt
     8401 tcagccagtg cttccagcgc ctgttcaaag cacgccagcg gtgcccgcac ggtgcctgcg
     8461 ccaatctgcc cgatccccgc ctctttatgg gcgataccgg tattgatgag tggcgtaatg
     8521 ccggtttcta ccacgcgacg aatgtccagt cccaggcacg cgccctgaaa atcccagctt
     8581 gggatctgca actgcatatt gcgttcaagg taaatttccg ccatctcttc agataccgcc
     8641 ctggccgctt ccatgccacc cgcaccgaca aagcgcgtta cgccaggcgc tgcgatcatt
     8701 gccgcacctc cgataccaaa ggtttcggta atcgcgctat cgcccatatc cgggttcgcc
     8761 tgctcctgcg agaagccggt gaaaaacaga ccttgcggag tgtttacagg cgcagtaaac
     8821 cagcgttcgc ccagcccgct tacccgaatc ccgaacatat tgccgttgcg ggtcattgcc
     8881 gtgacgatgc tgcctgcgcg gatcatcgcg ccagcatcca tcgccgcctt gcagtaagcc
     8941 atcgcgaggt tgaggaagaa ctgatcggtc acgctgagga aatccatcac ttccgcgatg
     9001 tgctgtttat catgatcgag gcgagcaatt tgtggggcca gcgcacgcat taacagtgct
     9061 gaggaagcaa tattgcgttg atggaactca tcgcccatcg taatgccctg cgccatcatc
     9121 gcggtaagat cgataccgcg ttccatgcgc cccagcgccg cgcttaatac tggcattaac
     9181 acatcgcgca tccagcgatg gcgagtcagg acatcttcgc cgtaagcgcc aaaacgcatc
     9241 actttgccga taccttcgtt gaggttgcag tacgcccggt tgccgtcggt cacgttctcg
     9301 accaccagca tcggcatact ggcagaagta ataccgccca ttggcccgac ggcattcaca
     9361 tggtgacaag gaatgaagtt cacttccccc tgctccagta ttgccagcgc ctgcgcttca
     9421 tctttcgccc agccttcgaa cagacatgcg cccacgcacg cccctttcat gggtccggtc
     9481 atctcctgcc agcgcattgg cggcccggcg tgaagcagtg ttttgccctg gtttagttcg
     9541 ctgataagtg aagaagccgg ttgcacatcc agccagtgtg gacgagcgcg acgaatttgt
     9601 tcgataaccg cagcattggc ttgcgccact gatgtaaaca tgggaacccc ttattgcaaa
     9661 cgttctaata aacgagccag ttttttattg ccaccggcga ctggcgacca ttggtaatgc
     9721 acaaccggtt tgctggcgct ttgtagctcc agcgcaaaac tgcgtaatcc gatgttaatc
     9781 acggcgacgt tttccaataa tgacggtgtg tgttgctgtg cggcaggcga gagcggatga
     9841 attaacgctg ccgccagcaa ggtggcttcc ggtagcgaac tcacgaccgc aatccccgca
     9901 tcttccagcg tggcgatttg ctgcgagcgg cattgcgggt cacgttcagt gcctgtcacc
     9961 gtggcaatgg catacagtgg ttgattgtct gaacgcgcag cacaggcttt ttgccaggcg
    10021 cttaccagcg aggcggcagg atcggcagtc gcaccgaagc caatcacgac atcaagcagc
    10081 agcacacgca cttgcggttt agcgccgaga tcggcaatta actggttgcg taaggttggg
    10141 tcgatcatcg gatggggacg cccgacggtg tagaaatcat cgccgaggtc aataatctgg
    10201 tggccatcgg cgtccagcat catgccgtga tgatgggcat catcggcttc cacgccaagg
    10261 tttccggcaa gtaatcccgc cgcttcggca gccagcgtac cgccggtata caaaccgcaa
    10321 ataaatccgc tgctggcagg cgttatcgcg ttacgtcgcg ccgtaacgcg tgaaagcagg
    10381 caagccaggc gtgcggcctc atccagcgag gaggcaaacc agacattctc gtcgcgggcc
    10441 accgccgggg tataacctaa acacagcgcc accgtcggtt tgccggttgc tttcatggca
    10501 ttaacaattt tcagacgcac cgcttcggca ggtggttttg aaacaaatgc cagcacttcg
    10561 cttttctcgt ctgcactgag catttccagc gccgtcagcg cactgatgcc gcccacttca
    10621 cggctgaggt cgcggccgcc aaggccaatc gcgtgagtaa ttccctcccc tgccagcgca
    10681 atctgcgagc acagctcctg aatcccggta ccggaagcgc caatgacgcc aatgttgcct
    10741 tccggcatca cgttagcaaa agccagcggc gtgccggcaa tcatcgacgt accgcagtcc
    10801 ggccccatca ccagcaagcc tttttcccgc gcgcgggttt taagttggat ttcatcttcc
    10861 agcgtgacgt tatcggagaa catcatcacg ttgaggttgc gatccagcgc ctgattcgcc
    10921 agctccgccg catactcgcc agccactgaa atcagcgcca gattggcatc gggtaatttc
    10981 tgacaggcac tgtcccagcg acgcacctgc gtcaacgcct ggctgctgcc tgccccctgc
    11041 gccagttgtt ttagcgcctc ttcaagctgc tgcataatcg cctgcgcgat ccccgcatcc
    11101 gccgcttcgc tacgaattgc cacgcaaata tcgttcggcg tggcgttatt aaaatcgtca
    11161 tgcctgaaac ctgtggtatc taataacgct ttattggcgg gcgtacccat cattacggaa
    11221 acatcatcaa cattttctga ttcgctgagt tttcgtgaaa taatcattaa actgaccgaa
    11281 tcctgaaaac accctttttt aataaaggcg tggatcataa caactcctta aaaattgcac
    11341 ccatggcaat tgcacgagca gtgttaatgt ggcgattacg gtatcgatga caacgggtaa
    11401 ataaggtgat gaatatcaca cagggattat taacaaaaga attcaaataa ccgccatgct
    11461 attacgtgtt attttttaat aagcgtggtt gttttaaaaa ccaggttaaa agcgagcgga
    11521 ttatcaaata aattctaact atattttttt gcctgtctgg atcacataat ccagatattt
    11581 tccctgtatg ttaattgcag tcatgcttca caccgtcgtt aaaaaggaag acagatgaaa
    11641 atcagtcggg aaacactcca ccagctaatt gagaataaac tctgccaggc tgggttaaaa
    11701 cgtgagcacg ctgcaaccgt ggctgaagta ttggtttacg ccgatgccag agggatccac
    11761 tctcatggtg cggtgcgcgt ggaatactac gcggaacgca tttcaaaagg cggcaccaac
    11821 cgcgaaccgg agtttcgtct tgaggaaacc gggccgtgct cggcaatttt acatgccgac
    11881 aatgccgccg gacaggtcgc ggctaaaatg ggtatggaac atgccatcaa aaccgcccag
    11941 caaaatggcg ttgcggtggt cggtatcagc cggatgggtc acagcggcgc aatctcttat
    12001 tttgtgcagc aggcagcccg cgccggatta attggcattt cgatgtgcca gtccgatcca
    12061 atggtggtgc cttttggcgg cgcggaaatt tactacggta ctaacccact ggcctttgcc
    12121 gcgccgggag aaggcgacga gatccttacc tttgatatgg cgactaccgt acaggcatgg
    12181 ggaaaagtgc tcgacgcccg ctcgcgtaat atgtctatcc cggatacctg ggcggtcgat
    12241 aaaaacggtg caccaacaac cgatccgttc gcggtacatg ctctgctccc cgcagccggg
    12301 ccgaaagggt atggcctgat gatgatgatt gacgtcctct caggcgtctt actcggctta
    12361 ccgttcgggc gacaggttag ttcgatgtat gacgatttac acgccgggcg taatttgggg
    12421 caattacatg tagttattaa cccgaacttt ttctcctcca gcgaattatt ccgtcaacat
    12481 cttagccaga ccatgcgcga attaaatgcc attacccccg cgcccggttt taatcaggtt
    12541 tattatcccg gacaggatca ggatattaaa caacgcaaag ccgccgtcga aggcatcgaa
    12601 attgttgatg atatttacca gtatttaatt tccgacgcgc tttataacac gtcatacgaa
    12661 acgaaaaatc cctttgcgca ataattattg agacaggact ttcttatgat tacacatttt
    12721 cgtcaagcca tagaagaaac gctaccctgg ctttcctcat ttggcgctga cccaacgggt
    12781 gggatgaccc gtttacttta ttcaccggaa tggctggaaa cccagcagca atttaaaaaa
    12841 agaatggcag caagcgggct ggaaacacgt ttcgatgaag tggggaattt atacggtcgc
    12901 ctgagtggca ccgaatatcc acaggaagtg gttctgagcg gttcgcatat cgataccgtg
    12961 gttaacggcg gtaacctcga cgggcaattc ggcgcgctgg cggcgtggct ggcaattgac
    13021 tggctggaaa cgcaatacgg cgcaccgctg cgtacggttg aagtggtggc gatggcggaa
    13081 gaagaaggca gccgcttccc gtatgttttc tggggcagta aaaatatctt tgggctggcg
    13141 aatcctgacg acgtgcggaa tatctgtgat gccaaaggaa atagttttgt cgatgcgatg
    13201 aaggcttgcg gatttactct gccggacgcc ccgctaactc cgcgtcagga tattaaagcc
    13261 tttgtcgaac tgcatatcga acagggctgt gtgctggaaa gtaatgggca atcaattggc
    13321 gtggtgaatg caattgtcgg gcaacgtcgc tatacggtga cgctgaacgg cgaatcaaac
    13381 catgcaggca ccacgccgat gggttatcgt cgtgatacgg tttacgcttt cagtcgcatt
    13441 tgccatcagt cgatcgaaaa agcgaaaaag atgggcgacc cgctggtgct gacctttggt
    13501 aaggtggagc cgcgcccgaa tacggtgaat gtggtgccgg gtaaaaccac attcaccatt
    13561 gattgtcgtc ataccgacgc cgccgtgctg cgcgatttca ctcagcagtt agaaaacgac
    13621 atgcgggcga tttgcgatga aatggacatt ggtattgata tcgatttatg gatggacgaa
    13681 gaacccgtgc cgatgaataa ggacctggtc gccaccctga cagaattgtg tgaaagtgaa
    13741 aaactgaatt accgggtgat gcacagtggt gccgggcacg acgcgcagat tttcgcgcct
    13801 cacgtgccga cctgcatgat tttcattccc agcatcaacg ggatcagcca taacccggcg
    13861 gaacgcacca atattaccga ccttgccgaa ggggtcaaaa cgttggcact catgctttat
    13921 caacttgcct ggcagaaata aggagtcata aatgggatat ttaaataacg tcaccggtta
    13981 ccgcgatgat ttactggcta accgtgcgat tgtgaaacac ggtaatttcg cactgttaac
    14041 accagacggt ctggtaaaaa atattattcc gggctttgaa aattgtgacg cgacaatcct
    14101 ctccacgcca aagctgggtg cctcttttgt tgattatctg gtcacactgc atcaaaacgg
    14161 tggcaaccaa cagggcttcg gtggcgaagg cattgaaacg ttcctgtatg tgatctctgg
    14221 aaatatcact gccaaagccg aaggcaaaac atttgcctta agcgaaggtg gctatcttta
    14281 ttgcccgcca ggctccttaa tgacgtttgt taacgcccag gccgaagaca gccaaatctt
    14341 tttatataag cgccgctatg ttccggtaga aggccatgca ccgtggctgg tttctggcaa
    14401 tgccagcgaa ctggaacgca ttcattatga aggtatggat gatgtgatcc tgctggattt
    14461 tctgcccaaa gagttaggtt ttgatatgaa catgcatatc ctctcttttg caccaggtgc
    14521 cagccatggt tatatcgaaa cacacgttca ggaacacggt gcctatattc tttccggtca
    14581 gggggtttat aacctcgaca ataactggat cccggtgaaa aaaggcgatt acatctttat
    14641 gggcgcttat tctttacagg ctggttatgg cgtagggcgt ggtgaagcgt tcagctatat
    14701 ttactcgaaa gattgcaacc gcgacgtaga gatttaagtc ataccactcg cctgattatt
    14761 tttagctatg ttggattttg ccacggttta taccgtggct ttttttgtag taaattcagg
    14821 taaaaaaaga tgcgatataa atcgtatccg ctttaaaggt aagggtcagt ttttaattcc
    14881 ctgacctatt ttaatggcgc aggcaatatt tcgcgcgctg ttgaagagat tggtttcacc
    14941 gctggcgagc acttcggcta aaggtgccag acgaggcaaa atgctgaata ccgcgtcaat
    15001 gccgtactgg tgcaccactt ccacgccatc acccaatacg ccagcaatcc caatcaccgg
    15061 tacattaaac tgcttcgcca ccgacgcaac gcccagcggc gctttaccgc ctgccgtttg
    15121 cgagtcaatg cgcccttccc cggtgatcac cagtgctgcc ccctgcaccg cctgctccag
    15181 gttgaccgca cgcagtacaa tctcaatacc cggtttaata tccgcattga gaaataccgc
    15241 cgccgcaata cccataccgc ccgcagcgcc gccgcccgcc atctgacaga cattaatttc
    15301 agtttgctgt tgtaaaacac gggcgtaatt ttgcagcccc tgttccagct cttcgaccat
    15361 ctccggcgtt gccccttttt gcgggccaaa caccgccgcc gcgccgcgtg cccctaccag
    15421 cgggttatca acgtcacatg ctacttcaat atggcagttc gccagacgcg gatcgcattc
    15481 ctccatctca atgcttgcca cgcgcgctaa attgccgccg ttagctgcca gcacctgacc
    15541 gtcggcatcg agaaaacgca caccaagcgc ctgcgccatg cccataccgc cgtcgaccgt
    15601 cgcactgccg ccaatgccga gaataatatg gcgaatgtcg ttatccagcg catgccgaat
    15661 taactccccc gtaccaaaac tggaggccag caacggatta cgcttttcag gcgcgaccag
    15721 catcaggcca cttgctgccg ccatctcaat caccgctgtt ttcccgtcgc cggtaaggcc
    15781 ataaaaagca ttcacttttt cgcccatcgg cccgcaggct tcaagcgtca cgatgttacc
    15841 acccgtcgcg gcgaccatcg catccaccgt cccttcgccg ccatccgcta tcggcaaaca
    15901 gatatagttc gcatcgggaa agagggtcga aaacccggct ttaattgcct gacaacattt
    15961 ttctgcactt aagctctctt taaaagagtc tggcgcaatg acaatcttca ttatcaatcc
    16021 tttttcaaca aattattcga tgcgttccca cggaataagg ttatccataa gaacagccag
    16081 taaaatcccg accaataagc cgttactgag cagaggacga agcgttaatg gaaggtcttg
    16141 cagatacaca ggcggtaatg ccattaaaaa tatgccgaca aataacggca atgcgagtcg
    16201 ataaatattg cgagcggtaa acgttatttg ctggctaaac accagcgcgg aaaagagtaa
    16261 aggcagataa gaaaccagca tgaccgcact actcacgggt aaagggatac tgcaaaacag
    16321 tcgcgtgagt gccggaacca gcgccaccag caggcaaata acgctgccat aaataaacga
    16381 acgccgcgtg taatcgccag tctgggttaa taaaccaatg gatgaaacaa acggtgaaaa
    16441 tggaattacc gccagcggta cggttatcag tgtcataaat ccggtcgcca caaagctacg
    16501 acgataacgc gtattccccg cgccctgctg cggataaaaa acatccgtgc cccgaatcgc
    16561 accgtaggta ttgctgatat ttaccagacc tgtaatcacc gccgtcagaa taattcccgg
    16621 cgacaaagca ccgccactgc cgagcgggaa ccactgccag tgcaactcac cggagagcga
    16681 atgcgaagaa ggaaagcaaa agtaccacaa caaccagccg gttatggtgc cgaccagcag
    16741 gccataacgg gcaaaacgtt gcggcagaaa gataatcatc gccagtacca ggcacatcac
    16801 cgccaccgag agcgcgaatg gtggtaactg aattttaaaa tttgggtcgg ctatgccaaa
    16861 cggcagcccg agcatacctt tgaaaaagat agtggtcagc tgtgcgccca gcatcaacat
    16921 aaacaagacc atcaccgacg gcgtaaataa ccgtgccagg cgatggccta atccgctaaa
    16981 accaatcaac atcgtcagca cgccggagag cgcaatcccc actgccaggc tggtggcgat
    17041 atcgttgatc ggtgtcccgc gcgatgcttc gccaagggtg atagttagga tggttcccca
    17101 ccacaggccc cccggccctt ccataatagc gcgacgatgg ccacagaacg cctgagcgaa
    17161 gcaggccagt gcggtagcaa gaaaagcata ttgtgtgagt gtaagcaggc tactttgcgg
    17221 caactgaaaa gcagaaagta gcgtaggagg aaccacaacc gtgttgcaaa aaataaagaa
    17281 aaaccactga aatcctgata acaggctttc gcggctgact gcaaaattga acataaacac
    17341 accttaaccg gagaatgccc gccgcaagga cgggcattgc aggggccaga ttactgctga
    17401 tgtttaagga taaattgacc tttcggcgca acagggaagc cctgttcgat gtcgtaaatc
    17461 acatcaccac gtaagatggt tttcgtgata cgcgcgccaa tggtacggcc aacatacggg
    17521 ctgactttgt ggcgatattc cagatcgtca ttggtaagaa catagctgct attcggctga
    17581 atgaagacga agtcggcatc ttttcctggg gcgatacggc ctttttgctg cagaccgaaa
    17641 atatctgctg cgttagtcgc cattaatttg ccgaacattg gcagagacat tccgcgtttc
    17701 tgtaccgctt catcgaacat cacgtccata cagttttgca gaccggcaat accgccccat
    17761 gcttccatga tgttgccggc tttcatttcc ggagggcatg gtgagtggtc ggaaaccagg
    17821 cagtcgattt caccgttaaa cagtttttcc cacatgcctt tctggttttc cagatcgcgg
    17881 atcggcggtg aacacttcgc cagagtgccg atttcttcga actgatcggt atccagtaca
    17941 aagtaatgcg ggcaggattc acaagtaaca tcctgacctt cctgacgtgc acgagtcact
    18001 tcttcaacgc cttccgggct gctgatatgg caaacgtgca gacggcaacc ggcaactttc
    18061 gccagataca gcacgcggcg aatcgcttcc acttcggtaa ataccggacg cgaagccaca
    18121 tagtcatggg cagttacgcg accttcacgt ttcgcttctt cgcccagtgc gtcacagatc
    18181 agcgcgtttt cgcagtgcac cagcaccggc tgccccagtt cgcccagctt ctgcgcgcct
    18241 ttgaagaact gccagtcatt tacatcacgg aagtcgttgt cgataccgcg atcgccacag
    18301 gtcgcaacga agcatttgaa gccgacaacg cccacttcat ccagctcatg cagacggtcg
    18361 atgttgtaag acaccaggcc accgagttgt gccgcatcga tagtcagctt gcctttagcg
    18421 gcatcgaact tcagttcaat tgaagcgcgg tcaaccgttg caggcagctg gttgagcggc
    18481 atttcgatca tggtggtgat accacctttt gccgctgcgc gagtaccggt ttcataacct
    18541 tcccagtggc tacgacccgg ttcagaaata tgggtgtgcg catcaaccat gcccggcgaa
    18601 accaccagac cagacgcatc cataacttct tttgcatcgc ccagatcctg accgatagca
    18661 gcaattttgc cgcctttaac ggcgatatct acaacgcgag cttcgttttc taaaataacg
    18721 gtgccgtttt taatgattaa atcaaaagac ataacaaact ccttgtaaaa tacagattgg
    18781 tttttaactc aatgttcaat atcgggatta attaacctat ggttttttgc tctcctgttt
    18841 tttctgctgt tgtacgtttc tttaataagg cgtaggccgc aaaggcgacg atgacgccga
    18901 caaaccatga gacacgcgat aacggttcca taaagggaat aaacttaccg ccaagagata
    18961 aaataacggc caccagagtt actgaaaacg cggtgaggtt aaaaccgtta tcgtaatatt
    19021 tataatcgcc aggtgcggtg tacagttcat caagattaat ttgcccacgc atcaccacaa
    19081 aataatgcgc catcatgaca ccaattaccg gaccaagcat tccgccgata atatcgagga
    19141 acagataaat actgtcctga ttttccatta atttccacgg gcagatcagc aggctgataa
    19201 tactggcaat cagtacgccg tttttatagg tcagttttgt cggtgcaatg gcagcaatct
    19261 gataaccagc cggaataata ttaccagtgg cgttagtgga gatagtcgtc atcagaataa
    19321 ccagtaccgc aaagaacgag gcaaacaggc tgtcccaacg ctgaacaata tccagcacgt
    19381 tccaggtatc agcgccgtaa tgaatactgg ctccggcaat aatacatacc ccggcgaccg
    19441 caaacagaat ataggccaca actaaaccca gcgtttgccc cagcgcttgc tcacgaaacg
    19501 agtgggcgtt ttgcgtaaag tcggatgcgc tcaccgccgg agccgcccag accgcaacta
    19561 ccgcgttaat caccaccagg aacaggaagc cactgttttc tgctttctga ataccgctcg
    19621 gaatgtagtc aaagattgga ccgagcccga ccagcgaaat cgcccaaatc gccataccgc
    19681 cgaaaacgat atagatgcac gggttaagaa tggcagtgaa tttatttaaa actttgccac
    19741 cgccaaaacc tataccaacg ttgaccagcc agaagagtaa gaaagtaatt aagcccggta
    19801 gagaaaggcc taacagagtg aaatcaccac cgagagtcaa aaatcccggc cagattttgc
    19861 caatcagaat caagcaggcc agtgaccccg cgtaacattg caggccaaac cacataatgg
    19921 cagcaatacc gcctcttaat aatccgggaa acagtgcgcc acgtacgccg taagaagcac
    19981 gcaggatcat ggcaaacggt acgccgtatt tactgcccgc agcgccgttt aataccatta
    20041 ccgcggcaat gaaaaaggcg ctgaggataa ttgccagcat aatactaaag gtagacaagc
    20101 cgagaataaa aaagccgccg accatcacat aattgggaac gttatgaacc gaacccatcc
    20161 ataaggtaaa atagttaaat gttttccagg tccgctggct ttgcgttttc ggcaatagat
    20221 cttcgctata gccgcgttgc tggaatagtt ttctctgatg ttccataaat ccctcttaat
    20281 ttcacgtatc gaataacgtt gttttaatct agctatgagg aacaaataaa ataaaaatat
    20341 tatttttaat actgaagcaa catctttggc tgctttcttt tttaatattg ctggccgatt
    20401 cattttttga tatcggccat ttttattgcg ggtatcaggc cagtttatgg ttagccatta
    20461 attccagcgc ctgcactaac gcagagtgat ccaactggct gccaccgttt gccgcgcagg
    20521 tattaaataa ctcctggcag gtcgcagtgt ttggcaggtt cagcgccagt gccttcgcac
    20581 tttgcagtgc caggttgaga tctttctggt gcagcgcaat tttgaaacct ggattaaagg
    20641 tgcgtttaat catgcgctca ccgtgaactt ccagaatacg tgaggaagca aagccgccca
    20701 tcagcgcctg gcgcacacgt accgggtctg caccggcttt tgaagcaaac agcagagctt
    20761 cagagaccgc ttcaatattg agcgctacga taatctgatt tgccactttg caggtttgac
    20821 cgtcgccgtt accgcccaca agggtgatat ttttaccgag cagttcaaac agcggtttaa
    20881 cacgttcaaa taccgcttcg tcaccgccaa ccataattga caacgtccct tcacgcgcgc
    20941 cgatttcgcc gccggagact ggcgcatcga gataatcacc acccagttca ttcacctgac
    21001 gagcgaaacg tttagtttca atcggtgaaa tggagctcat atcaacaatg gttttgccct
    21061 tcagcgaggc tttggtacaa ccattttcac caaacagaac ttcttcaacc tgaggtgtgt
    21121 ccggcaccat aataaaaatg atgtccgctg cttccgttac ctggcgcgcg gtgtcgacat
    21181 taacagcacc gagtgacagt aattcatcag caaccggtcc aatggtcgtg acatgtaatt
    21241 gatgaccggc acgcgccaga ttaatggcca tcggagtacc cataatgcct aagccaataa
    21301 atcccagttt cataattaac ctctttttaa atgtcgctat tcctgaattc agacaacacg
    21361 atcccctgcc aacttagcgg cctaaaaaag cattgtctga atagcgttac gttttaacgg
    21421 tacggatcca tccagcgtaa accggcttcc gtggtggttt ggggtttata ttcacaccca
    21481 acccaaccgt tgtagtcaga attttcgatt accttaaaga gataatcata attaatttct
    21541 ccggtccccg gttcgccgcg atgcggatta tcagcaattt gcaggtgacc aattttatcg
    21601 gcccactgag tcatggtgtt ggttaattca ccttccatcc gctgcatgtg ataaatgtca
    21661 tactgaattt tcaaattgca gcaaccaaca tcatcaatca atttcagcgc ctgccgagtt
    21721 ccggtgagat ggaaaccagg aatatcaaaa tggttaatag gttcaatcag taataaaata
    21781 tcttctttca tcagcatatt cgcggcataa cgcaggtttt ctacaagcgt tgcgtgaatc
    21841 tgttcgctgc tgaaaccagc cggcgtttta ccgaccagac agttaatttt tttattaccc
    21901 agcgcacggg cataacgaat cgctgctgct acgccatccc gaaactcttc ttcacggcca
    21961 ggaatacagg caataccgcg ctcccccgcc gcccagtcac ccgccggtaa attgtgcagc
    22021 gtatgttcga gtttattact cgccagcacc tgttttaatt cttcaatgtc gtagtcataa
    22081 ggaaacataa attcaacgcc gcgaaaacca cactgcgcag ctttctcaaa acgggcgaga
    22141 aaatcatatt caccaaataa catcgataaa ttagcagaga aacgtaacat tatttatctc
    22201 ccttattcat agtgcatgaa gcaggtttca gtcggtgcat ccgctgcgtt atcggcgata
    22261 tcttcaaatt ccatgacgtt atccagttcg ctgcccatcg aaatatttgt cacacgctcg
    22321 agaataactt ccacgactac cggtacccga tattgcgcca ttaaggcttt cgcctgttca
    22381 aaggccggtg caatatcttc cggtttgaag acgcgaatag ctttacaacc taaaccttcc
    22441 gctactttta cgtggtcgac gccgtaaccg ttcacttcgc tggagttgat attctcgaaa
    22501 gcgagttgca cgcagtagtc catgtcaaag gcgcgttgtg actgacgaat caggccgaga
    22561 taggcgttat tcaccagcac atggatgtac ggaatgttga actgcgcgcc aacggctaac
    22621 tcttcaatca ggaactggaa gtcgaagtca ccggaaatcg ccaccacatt gcgtttcgga
    22681 tcagcggcac aaacccccag cgccgccgga atcgtccagc ctaacggacc agcctgacca
    22741 cagttgatcc agtggcggtc tttaaagaca tgcagcattt gtgccgcagc gatttgtgac
    22801 aggccaatgg tggtgacata acaaacatcg cgaccaaagg ctttgttcat ctcttcatac
    22861 acgcgctgcg gtttcaccgg cacgttgtcg aaatgggttt tgcgcagcaa agtgcgtttg
    22921 cgctgctggc agtcggcgac ccattcttta cgacacggca gacgacccgc tttttgcatc
    22981 tcctgcgcca cttcaaccag cagtgtcagc gccgctttag catcagagac aatgccgaga
    23041 tccggacaca gcacgcgacc aatttgcgtc ggctcaatat cgatatgcac gattttgcgc
    23101 ccttcggtgt atttctctac cgaaccggta tgacggttag caaaacggtt accgatacca
    23161 aacaccatgt cggacgccag cagcgttgcg ttaccgtaac gatgcgcggt ttgcagaccc
    23221 accatcccgg ccatcagttc atgatcgtcc gggatacagc cccagcccat cagcgttggg
    23281 atcaccggaa cgctggttag ttcagcaaac tgttgtaaca gtgcagctgc gtcagcattg
    23341 attaccccgc ccccggcaac aatcaccgga cgttcggcct ggattaacat ttctacagct
    23401 ttttcgatct gcatacggct ggcagcaggt ttgtagaccg gcagcggttc gtacatgtca
    23461 ggatcaaact cgatttccgc aacctgaacg tcgaacggta aatccaccag taccggaccc
    23521 ggacgaccag aacgcatcag gtgaaatgcc tgttgcagca cgcgaggcac cagcgccgct
    23581 tcacgtactg taaccgccat tttgctgacc ggtttagcaa ttgcttcaat atcaacagcc
    23641 tgaaaatctt ctttatgcag acgggcgcgc ggagcctggc cggtaatgca cagaatagga
    23701 atggaatcag cagaagcaga atagagcgcg gtgatcatgt ccgtgcccgc aggaccggaa
    23761 gtccccagac atacgccgat attccctgcc gttgcgcggg tataaccttc cgccatgtgc
    23821 gaagcacctt ccacatgacg cgccagaatg tgacgaatac cgccgtgctt acgcatcgct
    23881 gagtagaacg gattgattgc agctcccgga acaccgaagg cggtggtgat accttctttc
    23941 tccagcacat acattgccgc gtcaacggct ctcatttttg ccattttatt cctacctcca
    24001 ttaattggaa aaattttcca actttcaaat tttgtcaaaa aaacccgctc cgcagagcgg
    24061 gatgcgatag acattatgga tgtgctttca gtcccaacgc cgtgctgata tcgcgggcgg
    24121 tgtctctgac cagctcaccc tgactgacaa aacgatcttc tgtcagtctt gatgaaggcc
    24181 cggagatgga gatagcggca acaacactac ccacatcatc gtaaattgct gaagctatgc
    24241 aattcagacc tacaacatgc tcttctttat ctacggtata gcccagttca cgcgcttgtt
    24301 ccaggtcctt cagcagggtg ggcatatcca caagcgtagt tggcgtaaac tgctgcaaac
    24361 cggtttgcag aatgatgctc atcaactcct cttccgccag cggataaagc agcgctttgc
    24421 ccgcaccgga agcatgcagt ggcagacgac tgcccagtgg cgcacacatc ctgaccatcg
    24481 atttacactc taactgacca attaataccg cttcattgct gttacggatc gcgacattga
    24541 ccgtttcgcc agaaagtaac atcaggcggc gcataaacgg cccggcgacg gagaggacat
    24601 cgcggttatg gatgtacgct gcaccgacgt taaagacacc taatcctata tgccaccagc
    24661 ctaactgact gtcctgatag acaaaatccg ctgcctgtaa aaccttcagc aagcgaaagg
    24721 tcgtggagag cggcaaatcc agattgagag aaatatcgct aaccgacgaa cttcccccac
    24781 ttttttccag atattgcaga atcgcaattc cccgctctaa tgcctgtgcg cccttctgtg
    24841 ccacaggctc cgcctgtcct ggcctgccgc gccgtctaac ttccgtcata cctacgttaa
    24901 ctccgaccta caagtgagca taaacaatga acaattagtt tatcttatgc aagtcggttg
    24961 caggcgtcac gcaaaacaga gttcctgttc aggaatactt tcaacatcac agttgtcact
    25021 gccgccgcga tcgatggtca gaaaatcggt gacgcgctgc caggcgaaaa gtgggtgatg
    25081 ccagacgtta cgatggtaat tcactccctg ttcgccgttg gtaataaacg cccgcagggt
    25141 tgacaggtct ggtttgtcgt cacctaacgc cacgaccacc acaaacacct cacctttcat
    25201 cgggataaag gcctgagtac ccagcggatg acgctcgagt tcgtgaatgg tcagcggcag
    25261 attcgccggt tgcgcgcggt taatgctgat aagcgtacgg tcttgctcaa gaatctcaac
    25321 cagcgccaaa tcgtggtaac gctccaccag gccattgtta atatggaaaa aatcccgttg
    25381 ctgcgtttcg attacgtcgc cataagcact aaaggcttcc tgacttaacg gtaatacctg
    25441 aagtttcatc tttttgtcct tgtaacataa ttccgttcaa ccaccgccga ccggtcagct
    25501 tggtttcaat aacaggcaat ctaaaacctt aagagaacaa catcaataca aaagttggaa
    25561 tatttttcca aaattagatt tagtccacaa attgaacagg gttatcatgt aaaaatactg
    25621 ataaatcaga ggataagaaa atcacgcaac ttgcggattt aaaaggggat acctgaagat
    25681 gttcgatcca gaaaccttgc ggactttcat tgcggttgct gaaacaggaa gtttttcaaa
    25741 agcggcagaa cgattatgta aaaccacggc gacgatcagt tatcgcatta aacttctgga
    25801 agagaatacc ggagtagcgc tgtttttccg tacgactcgt agcgtaacgt tgacagcggc
    25861 tggcgagcat ctactttccc aggccagaga ctggctgagc tggctggaaa gtatgcccag
    25921 cgagctgcaa caggtgaatg atggcgtgga acgccaggtg aatattgtca tcaacaacct
    25981 gctctataac ccccaggccg tcgcccagtt gctggcgtgg ctgaatgagc gttacccctt
    26041 tacccagttt cacatctccc gacaaatcta tatgggcgtc tgggactcgc tattgtacga
    26101 aggtttttcg ctggctatcg gcgtcacggg aactgaggcg ctggcaaata cctttagtct
    26161 tgatccctta ggatcggtgc aatggcgatt tgtcatggcg gcggatcatc cgctggcgaa
    26221 cgttgaagag ccgctaacag aagcgcagtt gcggcgcttt ccggcggtca atattgaaga
    26281 cagcgcccgc accttaacca aacgcgtcgc ctggcgattg ccgggtcaaa aagagattat
    26341 tgttcccgat atggaaacga aaatcgccgc ccatctggcg ggcgttggca ttggtttttt
    26401 gccaaaatcg ctttgccagt caatgatcga taatcaacaa ctggtcagcc gggtaatccc
    26461 aacgatgcgc cctccttcgc cattgagtct ggcatggcgc aaatttggca gcggcaaagc
    26521 ggtagaagat attgtgacct tgtttaccca gcgcaggccg gaaatcagcg gatttttaga
    26581 aattttcggc aacccacgca gttaaaaatc aagtttcctt gcgcgttggc gcgtaaaata
    26641 gcgcgcaagc tgatttgagg ttcactttta actatgcaag agagacacac ggaacaggac
    26701 tatcgtgccc tgctgattgc tgatacgccc attattgatg ttcgcgcccc tatagagttt
    26761 gagcaaggcg caatgcccgc cgctatcaat ctgccgttaa tgaataacga tgaacgcgcc
    26821 gccgttggca cctgctataa acagcaaggc tcagacgcag cgctggcgct gggacataaa
    26881 ctggtggcgg gtgaaattcg tcagcagcgc atggacgcct ggcgggcagc gtgcctgcaa
    26941 aatccgcaag gtattctctg ctgcgcccgt ggcggtcagc gctcacatat tgtgcaacgc
    27001 tggttgcatg cagcggggat tgattatccg ctggtggcag gcggttataa ggcactgcgc
    27061 cagaccgcga ttcaggcgac tattgaactg gcacaaaaac cgatagtgct gattggcggt
    27121 tgtaccggca gcggtaaaac gctgttagtg cagcaacagc cgaacggtgt tgatctggaa
    27181 gggttggcgc gtcatcgcgg ttcggcgttt ggtcgcacgt tacaaccaca acttagccag
    27241 gcgagttttg aaaacctgct ggctgccgaa atgctaaaaa ccgacgcccg tcagaatttg
    27301 cgcctgtggg tgctggaaga cgaaagccgg atgatcggtt cgaatcacct gccggaatgc
    27361 ctgcgcgagc gaatgactca ggcggcgatt gcggtggtag aagatccgtt tgagatccgt
    27421 cttgagcgcc tgaacgaaga gtatttcttg cgtatgcatc atgattttac ccacgcgtac
    27481 ggcgacgaac agggctggca ggaatattgc gaatacctgc atcacggact ttcggctatt
    27541 aagcgtcggc tggggctaca gcgatataac gaactggctg caaggctgga tgcagcactg
    27601 acaacgcaac tcaccaccgg cagcaccgac ggtcatctgg cctggctggt gccgttactt
    27661 gaagaatatt acgacccgat gtatcgctat cagcttgaga aaaaagcgga aaaagttgtc
    27721 tttcgcggtg agtgggcgga agtggcggaa tgggttaagg cgcagtgatg agggacgtat
    27781 ttgtattagt cgcgacttga tctgacacat ggccttgaaa ggttgagagt taccggtttt
    27841 gatatgagtg tcgaatcctt atccaaaaca agaggtaact ctcatgcttc atactgccaa
    27901 tcccgtcatc aaacacaaag ccggtttgct caatctggct gaagaactca gcaacgtatc
    27961 aaaagcctgt aaaatcatgg gcgtttcgcg tgatacgttt tatcgttacc gcgaactggt
    28021 cgctgaaggc ggtgtggatg cgcagattaa tcgtagtcgc cgcgctccta accttaagaa
    28081 ccgtaccgat gaggcaactg aacaggctgt tgttgattac gccgtcgctt tcccgacaca
    28141 tggtcagcac cgggccagca acgaactgcg taaacagggg gtttttatct ccgacagtgg
    28201 tgtccgttct gtctggttac tccataacct tgagaatctc aaaagacgat actgaacaaa
    28261 ttttatcagg tgacgttccg taaaaagttg tatggagttc tcgatgcagt cggcgcgact
    28321 atcagtaccc ccgctgaacc ctggactatc gggccgccgt actggggcat gtcaccctgg
    28381 cgtgccgctg gttttccgct aatggtttat ttcctctttc tttcccggca agaatttatt
    28441 ttaatccgga aggattattt attttcagat agggactcac attaaacacg taacatttta
    28501 attaatgcat ggctgaatat gtattacaca atgtcagata tcgtaaaaga ccagtgcaat
    28561 tctatgtaaa ctctctgact gttcatttta tttgttgttt cagggtcgga actcaccctc
    28621 aataaacaac tgaatacgag tagatattca tcgtctgagc tatatggctt tacacaatag
    28681 ccgacatgtc ttttctcagt caggaatttt acttttcctc actacgaagt tgacgacttc
    28741 gatatgggat agactcttaa ttcaagcaat tactctgcac aagcatcagg caatccagct
    28801 ccaatatttg ttataaaata atgcgtaaca ttaatttcac caacaaaaaa aagatttacg
    28861 ctatcccctt gccctggtat tttaccatat ttacaccaca ctttttttgt atctggatca
    28921 ttctttgata cgattttcgc cacttgttcc ctggccctca ccgtcaatgt tttcttaaac
    28981 ct
//
LOCUS       DAZFKF010000058        28944 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFKF010000058 DAZFKF010000000
VERSION     DAZFKF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.058"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(101..424)
                     /locus_tag="ACHKA1_003030"
     CDS             complement(101..424)
                     /locus_tag="ACHKA1_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO5680649.1"
                     /translation="MKRTLLIAVWAIGLMSDGAMALTLNEARSQGRVGETLNGYLVAL
                     QTDAETQALVKDINEARNHSYQQLAKQNNVSTKEIAKLAGQKLVARAKSGQYVQGVNG
                     KWLRK"
     gene            complement(432..617)
                     /gene="ynbE"
                     /locus_tag="ACHKA1_003031"
     CDS             complement(432..617)
                     /gene="ynbE"
                     /locus_tag="ACHKA1_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO5680650.1"
                     /translation="MKIVIAAFASSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEDLLKTRSDLF"
     gene            complement(614..3253)
                     /gene="ydbH"
                     /locus_tag="ACHKA1_003032"
     CDS             complement(614..3253)
                     /gene="ydbH"
                     /locus_tag="ACHKA1_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO5680651.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVGAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQVSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANAGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNAQLPETGCPSGKECEEKQ"
     gene            3461..4450
                     /gene="ldhA"
                     /locus_tag="ACHKA1_003033"
     CDS             3461..4450
                     /gene="ldhA"
                     /locus_tag="ACHKA1_003033"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO5680652.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4561..4983
                     /gene="hslJ"
                     /locus_tag="ACHKA1_003034"
     CDS             4561..4983
                     /gene="hslJ"
                     /locus_tag="ACHKA1_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO5680653.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4980..5246)
                     /gene="ydbJ"
                     /locus_tag="ACHKA1_003035"
     CDS             complement(4980..5246)
                     /gene="ydbJ"
                     /locus_tag="ACHKA1_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO5680654.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5520..9044
                     /gene="nifJ"
                     /locus_tag="ACHKA1_003036"
     CDS             5520..9044
                     /gene="nifJ"
                     /locus_tag="ACHKA1_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO5680655.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKVRF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9409..10542
                     /gene="ompN"
                     /locus_tag="ACHKA1_003037"
     CDS             9409..10542
                     /gene="ompN"
                     /locus_tag="ACHKA1_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO5680656.1"
                     /translation="MKSKVLALLIPALIAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10683..11117
                     /gene="uspF"
                     /locus_tag="ACHKA1_003038"
     CDS             10683..11117
                     /gene="uspF"
                     /locus_tag="ACHKA1_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO5680657.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11293..12228
                     /gene="ttcA"
                     /locus_tag="ACHKA1_003039"
     CDS             11293..12228
                     /gene="ttcA"
                     /locus_tag="ACHKA1_003039"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO5680658.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(12357..13730)
                     /gene="dbpA"
                     /locus_tag="ACHKA1_003040"
     CDS             complement(12357..13730)
                     /gene="dbpA"
                     /locus_tag="ACHKA1_003040"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO5680659.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQQLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGYIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(13760..13933)
                     /gene="ynaL"
                     /locus_tag="ACHKA1_003041"
     CDS             complement(13760..13933)
                     /gene="ynaL"
                     /locus_tag="ACHKA1_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO5680660.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(14208..15191)
                     /gene="zntB"
                     /locus_tag="ACHKA1_003042"
     CDS             complement(14208..15191)
                     /gene="zntB"
                     /locus_tag="ACHKA1_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO5680661.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(15205..15252)
                     /gene="ydaN"
                     /locus_tag="ACHKA1_003043"
     CDS             complement(15205..15252)
                     /gene="ydaN"
                     /locus_tag="ACHKA1_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO5680662.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            15445..16677
                     /gene="dgcM"
                     /locus_tag="ACHKA1_003044"
     CDS             15445..16677
                     /gene="dgcM"
                     /locus_tag="ACHKA1_003044"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO5680663.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLVA"
     gene            complement(16698..17261)
                     /gene="smrA"
                     /locus_tag="ACHKA1_003045"
     CDS             complement(16698..17261)
                     /gene="smrA"
                     /locus_tag="ACHKA1_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO5680664.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(17591..18499)
                     /gene="abgR"
                     /locus_tag="ACHKA1_003046"
     CDS             complement(17591..18499)
                     /gene="abgR"
                     /locus_tag="ACHKA1_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5680665.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVREILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            18675..19985
                     /gene="abgA"
                     /locus_tag="ACHKA1_003047"
     CDS             18675..19985
                     /gene="abgA"
                     /locus_tag="ACHKA1_003047"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO5680666.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELH
                     QLGYSLALGREVVNESSRMGLPDEFTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YMVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            19985..21430
                     /gene="abgB"
                     /locus_tag="ACHKA1_003048"
     CDS             19985..21430
                     /gene="abgB"
                     /locus_tag="ACHKA1_003048"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO5680667.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPISKNVTPSPLK"
     gene            21467..22993
                     /gene="abgT"
                     /locus_tag="ACHKA1_003049"
     CDS             21467..22993
                     /gene="abgT"
                     /locus_tag="ACHKA1_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO5680668.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            23004..23519
                     /gene="ogt"
                     /locus_tag="ACHKA1_003050"
     CDS             23004..23519
                     /gene="ogt"
                     /locus_tag="ACHKA1_003050"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO5680669.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            23714..24466
                     /gene="fnr"
                     /locus_tag="ACHKA1_003051"
     CDS             23714..24466
                     /gene="fnr"
                     /locus_tag="ACHKA1_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO5680670.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            24618..25568
                     /gene="uspE"
                     /locus_tag="ACHKA1_003052"
     CDS             24618..25568
                     /gene="uspE"
                     /locus_tag="ACHKA1_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO5680671.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(25618..25875)
                     /gene="ynaJ"
                     /locus_tag="ACHKA1_003053"
     CDS             complement(25618..25875)
                     /gene="ynaJ"
                     /locus_tag="ACHKA1_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO5680672.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            26119..27150
                     /gene="ynaI"
                     /locus_tag="ACHKA1_003054"
     CDS             26119..27150
                     /gene="ynaI"
                     /locus_tag="ACHKA1_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO5680673.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(27201..28814)
                     /gene="mppA"
                     /locus_tag="ACHKA1_003055"
     CDS             complement(27201..28814)
                     /gene="mppA"
                     /locus_tag="ACHKA1_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO5680674.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKSGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT         7326 a         7274 c         7001 g         7343 t
ORIGIN      
        1 taaggttttt gtagaaatac ccacggtaat taagcatgct aacgtcagtt taaatactgt
       61 aaaagatcat taatagatac attttgtagc caactataat ttatttgcgc aaccatttac
      121 cattaactcc ctgaacatat tgccccgact tcgcgcgagc aacaagcttt tgtccagcca
      181 gtttggctat ctctttagtg gatacattat tttgctttgc cagttgttgg taactatggt
      241 tacgagcttc attaatatct tttactaatg cctgtgtttc ggcatccgtt tgtagcgcca
      301 ccaggtaacc gttaagcgtt tcacctaccc gcccctgact tctggcttcg ttcagggtta
      361 aagccattgc accgtcgctc atcaggccaa tggcccatac agctatgagt aatgttcttt
      421 tcatcatcac ctcaaaatag atcgctacgg gttttgagca ggtcttcaac gtccttatcc
      481 gctttaatga tgatttcatg ctcaatttta acgttcatat taatggtgat tggctctttc
      541 ggcgcagcca cttcaatgcg aggcgtgcaa ccaaccaaca taaaagagga cgcaaacgcg
      601 gctatcacta ttttcattgt ttttcctcac actcttttcc tgatgggcag ccagtttccg
      661 gtagctgggc attctgctcc agccatacct ggagattatc gccaaagcgt aaactgcgcc
      721 ataacgtaaa aatattctct tcatggtgat aattaaggtt taccgtaccg cttttaccat
      781 cgacgcgact ggtgcccgaa atgttggcct gcatggttaa cacaccgaga ttatctaaat
      841 taatccttgt tgatgagcga ctgatttcca tataacgcaa ccagttgatg gcagttccag
      901 cggtcatatt gtctttggcc accgcatctg cagtgtcttt atccagacgc agcgtcatcg
      961 gccccgcgtt tgccaaccag ccatctttga caatccattt ttcattgttc agccatagtg
     1021 gcaacgcccc actaaatgcc ccagacatag caaactgttt cggattaacc gcgctaacca
     1081 gttcgctgga tgagagatta ttcagccgca acagcgccgg gtcatgttgc ggcatacgta
     1141 attgtttcat cagcacgtta ccgcctaaca catcgacgct aacatcagtc aacagcaatg
     1201 gttcttcttc agtccacgga taacgccctt gcaaatcagc cgtaatattt ttcgctgtca
     1261 ccagattaat cacttcggca attcgcaacg taacggggcc gcgagtcccc agatgccagg
     1321 ctccatcggc aaaacggaaa ggcaggacaa aatcgacacc gttcacctga ttatctggca
     1381 tccaggcact accgcctttc aacacgccgt gccctcccgc gcggaatcct tgttcaggcg
     1441 cagcagaaaa tgcaacctga gcatacagtt caccatcgcg taagttcatc ttccagtcgg
     1501 gtggcaccag cggctggaat acagtcagtg attgtttagg ccaccaggca ttaccgcgca
     1561 gacgaatacc gtcccagcga ccatttaccc gaaccgggcc aatctcacca gcatgtaaat
     1621 cgcctttaaa gaggaaatac gtcggatcgc gcccatcgac gctaaatttt aaggttgatg
     1681 gcggtaacac actgccgcca gtgaacagcg tttgcccggc gtccagtgac agcgcgccgc
     1741 tgaaggaggg atgctgtgcg tcacgtaccc agacgacggg cttgtcgaga attaatcgcg
     1801 gcttttctac cgtcatcgta ccgtattgta actggtcgaa accggtggag agatcggtca
     1861 gcgtaatcgt gctgtcatgc cactcacctt ttcctgcgac atcccaggtg gcattcatcg
     1921 gtgtaaaact cccttttccc cagtagcgcc actgccagcg gccagcgtca gggagaaaat
     1981 cattcgccag cccatccata tgcagcacga aatcgcccag ttcattttca tgcgcctgca
     2041 aaatggcctg caaacgtccg tcaacaccac gttgggtgac ttttacaccc gctaaaggcc
     2101 agcggatttc atcgatgtcc agcgaatcga tgactcttcc ctttgaacga agtaacgcgc
     2161 ctggctcaaa ggtcagcgtt gggtcagaca gacttccact tacctgcgca ggtaaacggg
     2221 catataaaat gaggtccgcc tgtttcgctt caccggtcag ctgcagaggc agctgactgt
     2281 tatccatgct taattttcct gggccaaaat ttagcaccgc gttgccttta cccgcttgcc
     2341 cctgggtcag cacactcagt cgtccgctga ccagagcgtt ctcaagccct gcctgccagt
     2401 tgtcgacttt gacacccagt cggccactca aaggaaaacc tgcatacggc cagctccagc
     2461 gaccatcgct tacggtcaat tgttgacgcg taatttgcca cggcaaatcg agcaacggat
     2521 cgccgttatc ccgtgccagc acgatcaatt gcccgctatt ttcctgccag tccagctcgg
     2581 cacccaccag tgacggttcc tgcggcaagt ttaacgtcgc agtagcatgc ccacttacag
     2641 gaagtccatc cggcacgagc ggcatagtaa attcccccac cagtttaacc ggcggctgat
     2701 tttcaaacgc gacgacatcc agttcgctga ctgtaagttg ttgccctttc agctggcctt
     2761 gaaatttaac tttttcgccc tgataacgca gttgctggat atcagaggtt aatgcgagag
     2821 agagttttcc ctgccattcc tgccagggag aaaaaatcag tttatcgata ttgatccagg
     2881 tgttaggcag catggactgc cactgcgcga gggtttttgg agcagctggc gattgctccg
     2941 tctgcggcaa tttcgccaga caagcagaat caagttctac cgtgccgacg ttcaataacc
     3001 agcggctggg atgtgaaagg ctggcgttgg tgatatgcgc aagctgacaa tctcccacca
     3061 gataacggag atcgggaatg attaaacctt tacgcgtaat gcgtgggctt tcatctaatg
     3121 caatacgtgt cccgagcggt agccagatgc ccgccagcgt gggaacccac agcccgagcg
     3181 tcatcagcag cgtcaacggc acaagaataa tcagtaataa cagcgcgaga acggctttat
     3241 atttacccag catgggtagt taatatcctg atttagcgaa aaattaagca ttcaatacgg
     3301 gtattgtggc atgtttaacc gttcagttga aggttgcgcc tacactaagc atagttgttg
     3361 atgaattttt caatatcgcc atagctttca attaaatttg aaattttgta aaatattttt
     3421 agtagcttaa atgtgattca acatcactgg agaaagtctt atgaaactcg ccgtttatag
     3481 cacaaaacag tacgacaaga agtacctgca acaggtgaac gagtcctttg gctttgagct
     3541 ggaatttttt gactttctgc tgacggaaaa aaccgctaaa actgccaatg gctgcgaagc
     3601 ggtatgtatt ttcgtaaacg atgacggcag ccgcccggtg ctggaagagc tgaaaaagca
     3661 cggcgttaaa tatatcgccc tgcgctgtgc cggtttcaat aacgtcgacc ttgacgcggc
     3721 aaaagaactg gggctgaaag tagtccgtgt tccagcctat gatccagagg ccgttgctga
     3781 acacgccatc ggtatgatga tgacgctgaa ccgccgtatt caccgcgcgt atcagcgtac
     3841 ccgtgacgct aacttctctc tggaaggtct gaccggcttt actatgtatg gcaaaacggc
     3901 aggcgttatc ggtaccggta aaatcggtgt ggcgatgctg cgcattctga aaggttttgg
     3961 tatgcgtctg ctggcgttcg atccgtatcc aagtgcagcg gcgctggaac tcggtgtgga
     4021 gtatgtcgat ctgccaaccc tgttctctga atcagacgtt atctctctgc actgcccgct
     4081 gacaccggaa aactaccatc tgttgaacga agccgccttc gatcagatga aaaatggcgt
     4141 gatgatcgtc aataccagtc gcggtgcatt gattgattca caggcagcaa ttgaagcgct
     4201 gaaaaatcag aaaattggtt cgttgggtat ggacgtgtat gagaacgaac gcgatctgtt
     4261 ctttgaagat aaatccaacg acgtgatcca ggatgacgta ttccgtcgcc tgtctgcctg
     4321 ccacaacgtg ctgtttaccg ggcaccaggc attcctgaca gcagaagctc tgaccagtat
     4381 ttctcagact acgctgcaaa acttaagcaa tctggaaaaa ggcgaaacct gcccgaacga
     4441 actggtttaa tcttgccgct cccctgcatt ccaggggagc ttattcagat aatccccaaa
     4501 gacctttcat cctctattct taaaatagcc ctgagtcaga aactgtaatt gagaaccaca
     4561 atgaagaaag tagccgcgct tgttgcgcta agcctgctga tggcgggatg tgtaagtaat
     4621 gacaaaattg ctgttacgcc agaacagcta cagcatcatc gctttgtgct ggaaagcgta
     4681 aacggtaagc ccgtgaccag cgataaaaat ccgccagaaa tcagctttgg tgaaaaaatg
     4741 atgatttccg gcagcatgtg taaccgcttt agcggtgaag gcaaactgtc taatggtgaa
     4801 ctgacagcca aagggctggc aatgacccgt atgatgtgcg ctaacccgca gcttaatgaa
     4861 ctcgataaca ccattagcga aatgctgaaa gaaggtgcac aagtggatct gaccgcgaac
     4921 cagttaacgc tggcgaccgc gaaacagaca ttaacttata agctggcgga tttaatgaat
     4981 taatagctgc cacagctccc ggcggcaagt gactgttcgc tacagcgttt gccgttgggt
     5041 aatgcacaca tcccaatcgc cgtaccatcc agttgacggg caacagaaag cgaaccgccg
     5101 atcattgcac aatttgcttc tccactactg gacatcgacg cttttaaacc tggcgctacg
     5161 tgcgccgcag tcgcctgctg aacaggctca ctactacacg ccgacaacaa taaagcggca
     5221 caccctaccc aaaacgctgc tcgcatctct tcttcctctg atcttcaagc caaacgacac
     5281 cgccataaat aataggcagc acagagggcg acgtcgagag ctgtcctgcg cgttgccccg
     5341 catttttact tttttatggc tatttttttg ccctctgttt gatcaaaaca ttcattacgc
     5401 tgatgtgggg gacacaaaag cgaaaatgca gaagaaagcc atttgctaaa attgaaagat
     5461 tactactggg cgcgcagcaa tttcgtgcgc ccctcatttg cgcaatgtaa gggtgtcata
     5521 tgattactat tgacggtaat ggcgcggttg cttcggtggc gtttcgcacc agtgaagtta
     5581 tcgccatcta ccctattacc cccagttcca cgatggcaga acaggctgat gcctgggccg
     5641 gaaacggctt gaagaacgtt tggggagaca caccacgcgt ggttgaaatg cagtcggaag
     5701 cgggtgctat cgctaccgtg catggcgctt tgcagacggg tgccctttca acatcgttta
     5761 cgtcatcgca gggtttgctg ctgatgatcc cgacgctgta caaactggca ggcgaactca
     5821 caccgtttgt cctgcatgta gcggcacgta ccgttgccac acatgcactc tctatttttg
     5881 gcgatcattc cgacgtcatg gcggtgcgcc agacgggttg cgcgatgttg tgtgcggcaa
     5941 acgtccagga agcgcaagac tttgctctca tttcgcacat cgcgacgctg aaaagccgcg
     6001 tgccatttat tcatttcttt gatggtttcc gcacgtccca cgaaatcaat aaaattgtcc
     6061 cgctggccga tgacacgatt cttgatctca tgccgcaggc cgaaattgat gctcatcgcg
     6121 cccgggcact caacccggaa catccggtga tccgcggtac gtccgccaat cctgacactt
     6181 atttccagtc tcgcgaagcc accaacccat ggtacaacgc ggtctatgac catgttgaac
     6241 aggcgatgaa tgatttcgct gccgcgacag gtcgtcagta tcagccgttt gaatattacg
     6301 ggcatccgca agcggaacgg gtgattatcc tgatgggctc tgccattggt acctgtgaag
     6361 aagtggttga tgaattgcta acccgtggcg aaaaagttgg cgtgctgaaa gttcgtctgt
     6421 accgtccctt ctccgccaaa cacttgctgc aagctctgcc gggatccgta cgcagcgtgg
     6481 cggtactgga cagaaccaaa gaacccggtg cccaggcaga accgctctat ctggatgtaa
     6541 tgaccgcact ggcagaagcc tttaataatg gcgagcgcga aactctgccc cgtgtcattg
     6601 gtgggcgcta tggtctttca tccaaagaat ttggcccgga ctgtgtactg gcggtatttg
     6661 ccgagctcaa cgcggctaaa ccgaaagtgc gctttacggt tggtatttac gatgatgtga
     6721 ccaatctgtc actgccgttg ccggaaaaca ccctgccaaa ctcggcgaaa ctggaagcct
     6781 tgttttatgg gcttggtagt gatggcagcg tttccgcgac caaaaacaat atcaagatta
     6841 tcggtaattc cacgccgtgg tacgcacagg gctattttgt ttacgactcc aaaaaggcgg
     6901 gcggcctgac ggtttctcac cttcgagtga gcgaacagcc gattcgttcc gcttatctca
     6961 tttcccaggc tgattttgtt ggctgccacc agttgcagtt tatcgataaa tatcagatgg
     7021 ctgagcgttt aaaacctggc ggcattttcc tgctcaacac gccgtacagc gcagatgaag
     7081 tgtggtcgcg cttgccgcag gaagttcagg ccgtgctaaa tcagaaaaaa gcgcgcttct
     7141 atgtgattaa cgcggcgaaa atcgcccgcg aatgtggcct ggcggcccgt attaataccg
     7201 tcatgcagat ggcttttttc catctgacgc aaattctgcc tggcgatagc gccctcgcag
     7261 aattgcaggg tgcgattgcc aaaagttaca gtagcaaagg tcaggatctg gtggaacgca
     7321 actggcaggc tctggcgctg gcgcgtgaat ccgtagaaga agttccgttg cagccggtta
     7381 atccgcacag cgccaatcga ccgccagtgg tttccgatgc cgcccctgat ttcgtgaaaa
     7441 ccgtaaccgc ggcgatgctc gccgggcttg gtgatgccct ccccgtttcg gcgctgccgc
     7501 cagacggcac ctggccgatg ggcactacac gctgggaaaa acgcaatatc gccgaagaga
     7561 tccccatctg gaaagaggaa ctctgtaccc aatgtaacca ctgtgttgcc gcttgcccac
     7621 actcagctat tcgcgcaaaa gtggtaccgc ctgaagcgat ggaaaacgcc cctgccagcc
     7681 tgcattcgct ggatgtgaaa tcgcgtgata tgcgcgggca gaaatatgtc ttgcaggtgg
     7741 ctccggaaga ttgcaccggt tgtaacctgt gcgtcgaagt ttgcccggcg aaagaccgtc
     7801 agaatccaga gattaaagcc atcaatatga tgtcgcgcct tgaacatgtc gaagaagaga
     7861 aaatcaatta cgatttcttc ctcaacctgc cagaaatcga ccgtagcaaa ctggaacgta
     7921 ttgatattcg tacatcgcag ctgattacac cgctgtttga atattccggt gcttgctccg
     7981 gttgtggcga gacgccgtat attaaattgc tgactcagct ctatggcgac cggatgttga
     8041 tcgctaacgc caccggctgt tcttcaattt atggcggtaa cctgccctct acaccgtata
     8101 ccaccgatgc caacggtcgt gggccggcat gggcgaactc tctatttgaa gataatgccg
     8161 aatttggcct tggtttccgc ctgacggtcg atcaacaccg tgtccgcgtg ctgcgtctgc
     8221 tggatcaatt tgccgataaa atcccggcgg aattactgac ggcgttgaaa tcagacgcta
     8281 cgccagaggt tcgtcgagaa caggtcgcag ctttacgcca gcaactcaac gatgttgccg
     8341 aagcacatga actgctgcgt gacgcagatg cactggtaga aaaatcaatc tggctgattg
     8401 gtggcgacgg ctgggcttac gatatcggct ttggcggtct ggatcatgta ttgagtttga
     8461 cggaaaacgt caacattctg gtgctggata cgcaatgcta ttccaacacc ggtggtcagg
     8521 cgtcgaaagc gacaccgctg ggtgcagtaa ctaagttcgg cgagcacggc aaacgtaaag
     8581 cgcgtaaaga tcttggcgtc agtatgatga tgtacggtca tgtttatgtg gcgcagattt
     8641 ctctcggcgc gcaactgaac cagacggtga aagcgattca ggaagcggaa gcgtatccgg
     8701 gaccatcgct gatcatcgct tatagcccat gtgaagagca tggttacgat ctggcactca
     8761 gccatgacca gatgcgccaa ctcacagcta ccggcttctg gccgctatat cgcttcgatc
     8821 cgcgtcgtgc cgatgaaggc aaactgccgc tggcgctgga ttcacgcccg ccgtcagaag
     8881 cactggaaga aacgttactt cacgagcaac gtttccgtcg tctgaattcg cagcagccag
     8941 aagtggcaga acagttatgg aaagatgctg cagctgattt gcaaaaacgc tatgacttcc
     9001 tggcacaaat ggccggaaaa gcggaaaaaa gcaacaccga ttaaatgctc tggataagga
     9061 ttatccaatt ctaaaaaaag cccggacgac tgttcgggct tgtcttttta tatgttggaa
     9121 aatcagtggc aatgcaatgg cccaacagaa aataaaatct gtgacaataa aggcatataa
     9181 cccgcgcaga ataacgtata gaaaataaaa agtataattt ttattttgta tgtattatct
     9241 tcgatacatt caccagactt attatcattt cagaggaatt atttgattaa ggttttactt
     9301 aaggcgtaac aaatgatatt tatcggctaa ctgaacttct cctttaggat gttttcactc
     9361 cccgaaatgg ggataaaagg caaataaaat aactaaagga tttattcaat gaaaagcaaa
     9421 gtactggcac ttttaattcc tgccctgatc gccgcaggtg ctgcacatgc agccgaagtt
     9481 tataataaag acggcaacaa attagatctg tatggcaaag ttgatggcct gcattatttt
     9541 tctgataatt cagcgaaaga tggcgaccag agctatgcgc gtctgggttt taaaggcgaa
     9601 acccaaatta acgatcaact tactggctac ggtcaatggg aatacaatat tcaggcaaac
     9661 aacactgaat cttcaaaaaa ccagtcatgg acccgtctgg catttgccgg actgaaattt
     9721 gcagattacg gttctttcga ttacggacgt aattatggcg taatgtacga catcgaaggc
     9781 tggaccgata tgctgcctga atttggcggt gactcttata ccaatgcaga caactttatg
     9841 actggtcgtg ccaatggcgt cgcgacttat cgtaatactg atttcttcgg tctggtaaat
     9901 ggtctgaact tcgcggtgca gtatcaaggt aacaacgaag gtgccagtaa tggtcaggaa
     9961 ggcaccaaca acggacgtga tgttcgccat gaaaacggtg acggctgggg tctttccaca
    10021 acatatgatt taggcatggg ctttagcgct ggtgcggcat acacctcttc tgaccgcacc
    10081 aatgaccagg ttaaccatac tgcggcgggt ggtgataaag cagacgcgtg gactgctggg
    10141 ctaaaatacg atgctaacaa tatttacctg gcaaccatgt attcagaaac gcgtaatatg
    10201 accccgtttg gcgacagcga ttatgcagtt gcgaacaaaa cccagaactt tgaagtcact
    10261 gcacagtacc agtttgattt tggtctgcgt ccggcagtct ctttcctgat gtctaaaggc
    10321 cgtgacctgc acgctgccgg tggtgcagac aacccggcag gtgttgatga taaagatctg
    10381 gttaaatacg ccgatgttgg cgcgacttac tatttcaata aaaacatgtc cacttatgtt
    10441 gactataaaa tcaacctgtt ggatgaagat gacagtttct acgctgccaa cggcatctct
    10501 accgatgata ttgtcgcttt aggtctggtt tatcagttct aaatcctcct gcccgctgtt
    10561 atggcgggct ttttctgctt attcttcctc ctttgatcta aattaaaaat gtgaactccg
    10621 tcattacaca aaaagtgtca tctggcgtta cactttatgc ggatactaaa acaggaggtt
    10681 ttatgaacag aacgattctt gtccctatcg atatttccga ttcagaatta actcaacgcg
    10741 tgattagcca cgttgaggca gaggcaaaga ttgatgatgc agaggttcat ttcctgacgg
    10801 taataccttc actgccctac tatgcctctc tgggtttagc gtattccgca gaattaccgg
    10861 caatggatga cctgaaagcg gaagccaaat cgcaactgga agagatcatt aaaaaattta
    10921 aactgccaac cgacagagtg catgtccatg ttgaggaagg ttcgcccaaa gaccgtattc
    10981 tggaattggc gaagaagatc cccgctcata tgatcatcat tgcttcccat cgaccggata
    11041 tcaccactta tctgctcggt tccaacgccg cagctgtagt gcgtcacgca gagtgctccg
    11101 tgctggttgt gcgctgacac taacgcccgc acattgctgc gggctttttg attcatttcg
    11161 caaatgtgct gacattttcc cctctaatcc gtaccataca cgccacagtt tttatatcag
    11221 atttcttatg ctgggcgttc cggcatgacg catactcttc tgatgccata taacgaattg
    11281 agtcgctttt aaatgtcgca aaatcaagaa attagcaaga aagaacaata caatctgaac
    11341 aaattacaaa aacgcctgcg tcgtaacgtg ggcgaagcca ttgctgactt caatatgatt
    11401 gaagaaggcg atcgcatcat ggtttgcctc tccgggggta aagacagcta taccatgctg
    11461 gagattctgc gcaatttgca gcaaagcgcg ccaatcaatt tttcgctggt ggctgttaac
    11521 ctcgatcaaa agcaaccggg cttcccggaa cacgttctgc ccgagtatct tgaaaagctg
    11581 ggcgttgagt acaagattgt tgaagagaac acttacggta tcgtgaaaga gaagattcca
    11641 gagggcaaaa ccacttgctc actgtgttct cgacttcgtc gcggtatcct ttatcgcacc
    11701 gcaacggaac tgggggcgac gaagatcgcg ttgggccacc atcgtgacga tatcctgcaa
    11761 acgttgttct taaatatgtt ctacggcggc aagatgaaag gtatgcctcc gaaactgatg
    11821 agcgatgatg gcaaacatat cgttattcgt ccgctggcct actgccgcga gaaagatatt
    11881 cagcgatttg ccgatgcaaa agcgttcccg attattccgt gcaacctgtg cggttcacag
    11941 cctaacctgc aacgtcaggt gattgctgac atgttgcgtg actgggataa acgttatcca
    12001 gggcgtatcg agacgatgtt cagcgcgatg cagaatgtgg tgccgtcgca tctgtgcgat
    12061 accaacctgt tcgatttcaa aggcatcacc cacggttctg aagtggttaa cgggggtgat
    12121 ctggcgtttg atcgcgaaga gatcccacta caaccggcgg gctggcagcc agaagaagat
    12181 gaaaatcagt tggatgagtt acggctgaat gtggttgaag tgaaataacc aggatagcgc
    12241 ccgatgcgca agcgtatcgg gctactctta tggaggccgg ataagacgca gccagcgtcg
    12301 catccggcaa tcccgaataa gatgtttact cttgcacccg gcaattcaac atttcattat
    12361 tttaataacc gcacccggca cgtttttcct ttaatcttcc cgccctgtaa ctgtttccat
    12421 gctttatgag caacagcctg acggaccgcg acatagacat gcgccggatg cacggcgatt
    12481 ttgccaatat ctgcgccatc aagcccgata tatcctgtca gtgcacctaa tacatcaccc
    12541 gggcgcattt tggctttttt cccgccatcg atacacaacg ttgccatttc tgcttccagc
    12601 gtcacaatgg aactattagc tggcggcgtt tgccagttaa gttttatctg caacatgtca
    12661 gaaatgatat tggcccgctg tgcttcttcc ggagcacaga aactgatcgc cagaccgcta
    12721 tttcctgcac gagctgtacg accgatgcga tgtacatgaa cttcagggtc ccacgccagc
    12781 tcaaagttca ccaccagctc aagcgattta atatccagac cacgcgcagc aacatcagtc
    12841 gcgaccagta cacgggcgct accgttagca aaacgtacca gggtctgatc gcgatcgcgt
    12901 tgctccaaat cgccgtgtaa tgacaatgca ctttgcccta cttcattcag cgcgtcgcag
    12961 acagcctggc aatctttttt ggtattgcaa aacaccacgc aagaggatgg ctgatgcaag
    13021 cttaataact gttgcaacag aggaattttg cctttactgg atgtctcata aaattgttgt
    13081 tcaatgggtg gcaaagcatc tgttgagtca atttcaatcg ccaaaggatc gcgttgcact
    13141 cgtccgctga ttgcggcgat ggcttccggc caggttgccg aaaacagaag cgtctgtcga
    13201 gatgccggcg caaaacggat gacatcatca atggcatcgc taaatcccat atccagcatg
    13261 cggtcggcct catccatcac caaagtattc agcgcatcca gtgataccgt gcctttttgc
    13321 aggtgatcca gcaaacggcc cggcgttgcc acgataatat gcggcgcatg ttgcaacgaa
    13381 tcacgctgca taccgaacgg ttgaccaccg cacaacgtca aaattttggt atttggcaga
    13441 aaacgcgcca gccgacgcaa ttcacctgcc acctgatccg ccagttcacg cgtaggacac
    13501 agcactaaag cctgggtttg aaatagcgac gcatcaattt gctgtaacaa gccgaggcca
    13561 aaagccgccg ttttgccgct gccagttttc gcctgcacgc gaacatcttt tccggcaagg
    13621 atcgccggaa gcgcggcggc ctgcaccggc gtcatggtta aataacccaa ctcattaagg
    13681 ttcgtgagtt gggcgggagg caaaacattc agggtagaaa aagcggtcac aatctattct
    13741 cgtggtcatc gacgcaaagt tagcaggcgc gtatcctcgc agatctacgc tcacgatgcg
    13801 acaatttaat cggttcttca tcgggtggtg ggtcaggcat gggttgcggg cgagggatcg
    13861 gatcgggcac tggaacagga tcgccaggaa tcggttcagg gacaggaatt tgcaaataaa
    13921 taagtgtcgt catatttccc tccggtcatt gggtggactc ttaaagggta gacgctgata
    13981 aataacaggc aaaaaaaagc cgactcatca aagtcggcgt cgtacgaatc aattgtgcta
    14041 tgcagtaatt caaaaaagga agtaagacaa tatggagcgc aacgcccatc gcttgacgtt
    14101 gcattcacct gcaagagaga tattgccctg aatgggtaga gagtttattg acttcgctca
    14161 aactttgcgg cgtttttgta tacagatagc cggaaaaatt gcttttgtta caaccattta
    14221 ctacgatgca accataaagc aacaccacca ataagaacaa ctaacagaat acaaaaaatt
    14281 gaaaatccga attgccaccc gccgccaggg atcccaccaa ggttgacgcc aaataaccct
    14341 gtcagaaagg tactgggtaa aaagaccatt gccatcaatg acattgtata ggtacgacga
    14401 gctaaatttt cctgcatcac ctgagcgatt tcatccgcca tcacaccagt ccgtgctata
    14461 caggcgtcga tttcgtcaag gccgcgccca aggcgatcgg caatatcctg catccgacgg
    14521 cgttggtcat cgctcatcca cggcaaacgt tcactggcaa gacgagcata gacatcacgt
    14581 tgcggtgcca tatagcgacg catcacaatt aattgtttgc gcagcagagc caggaatcca
    14641 cgcggcggaa tttgctgatc aaggagatta tcttcaaggt cgataatttt atcgtgcagc
    14701 tgctcgataa attcgctgga atgatcggtc aacgcatcgc acacatccac cagccaccct
    14761 ccgcaatcgg tcggacccgt gccctcttcc agatcgctca ccacatcgtc cagcgccaac
    14821 actttgcgtt gtcgggtcga aacaattaac cgcccgtcca tatatacacg catggcgacc
    14881 agttgatcgg ggcgttcatc ggtgctgccg tttatacagc gcaatgtaat cagcgtgcct
    14941 tcaccgagac ggctgactcg gggccgtgtg ctctcacctg ccagcgcatc acgtacgtta
    15001 ttgggaagca gcggtgttgt cgccagccat tgggcgctat catggtgtac ataattaagg
    15061 tggagccagc agggatgcgc ttcatcaatc acatctgtat tttccagcgg tttaacgccg
    15121 cctctaccat ccagcatcca ggcaaatact gcatccggga cattaacgtc cgatccctta
    15181 atcgcttcca cagtgcctcc atcatcaacg cattattttg tagtctagcc ttctggccct
    15241 gttacgcaac atctcatcac cccattaccc tgaaatgatt aataaaattc tgtctaaatt
    15301 gaatacaaaa agcaaaatgc ttttccgtat acaaaccgtg tgaagtgtta aatagcgtct
    15361 atcattatca gaattatctg atcatatgac gtgctttttt gcgatcggat agcaacaaaa
    15421 attgataaaa ataacgggat ctcaatgatt acgcacaact tcaataccct ggacttactc
    15481 accagtcctg tctggatcgt ttcgcccttt gaggaacagt taatttatgc caatagcgcg
    15541 gcgcgactgt tgatgcaaga cctcacgttt agtcagctac gaaccggtcc ctattccgtc
    15601 tcctcacaaa aagaactgcc gaaatacctc tccgatctgc aaaaccaaca cgatattatc
    15661 gaaatcctca ctgttcagcg taaagaagag gaaacagcat taagctgtcg gcttgttttg
    15721 cgagagctga cagaaacaga accggtgatt attttcgaag gtatcgaagc gccggcaacg
    15781 ctgggtttaa aagccagtcg ctcggcaaat tatcagcgca aaaaacaagg tttttatgcg
    15841 cgcttttttc tgactaactc tgcaccaatg ttgttgattg acccgtcacg agatggccaa
    15901 atcgtcgatg ctaacctcgc cgcgctcaat ttctatggtt ataaccatga aacgatgtgc
    15961 cagaaacata cctgggaaat aaatatgctc gggcgtcgcg tcatgcctat catgcatgaa
    16021 atttcgcatt tacccggcgg tcataaacct ttgaattttg ttcataaact ggcggatgga
    16081 tctactcgtc atgtgcagac ctatgccgga ccgattgaaa tttatggcga caagctcatg
    16141 ttatgtattg tgcatgatat tactgagcaa aaacggctgg aggagcagct ggaacatgct
    16201 gctcaccatg acgcgatgac cggattactg aatcggcgac agttttatca cattacggaa
    16261 ccaggccaaa tgcagcatct cgccatcgct caggattaca gcttgttgct catcgacacc
    16321 gatcgtttta aacacattaa cgatctctat gggcattcta aaggtgatga ggtgttatgc
    16381 gccctcgccc gcaccctcga aagttgcgct cgcaaaggcg atttggtgtt tcgttgggga
    16441 ggcgaagagt ttgtcttatt gctaccaaga accccactgg ataccgcgct ttcgctggct
    16501 gaaactatcc gcgtaagcgt ggcaaaagtg agtatttcgg gcttaccacg ctttaccgtc
    16561 agcattggtg tggcgcatca cgaaggaaat gaaagcatcg atgaactgtt taaacgcgtt
    16621 gatgatgctt tgtatcgggc gaaaaatgat ggtcgcaacc gcgtgctggt ggcataagcc
    16681 gcggatgcgt ctcgagatca acgactgcgc ttagcgtggc gctcccagtt ttcttgcttc
    16741 gcctgcgccg ttttacgtag tgcgacgtaa cacgccccgc tgccgccatg atgcggtagc
    16801 gcggtgcaat atgcctgaac atcatcaaat tcggtcagcc agcgcgccac atagctgcgg
    16861 acaatattgg catgcgattt atcatcccgc cctttaccat gaataatcag cacgttacgc
    16921 aaaccatccg ccagggcttg ttgaataaaa ctgaacacca ttttgcggca ctcttccacc
    16981 ggctggcgca aaagattcag gctcgcctgt tgcggatatt taccactacg cagcttatcc
    17041 agcaccccat gttgcaaccc ttcccgccga aactccagcg gctgacttag cgggataatg
    17101 tcgagaaatc cggtagtgag gaaattatca agctgcagcg tgtcgatacg ctgcggcgca
    17161 cgttggttac gcgttggatg ccagtggaca tcggtagcac gtttcagcgg ctggacatct
    17221 tccatggcgt caagaaacag cgatttgtcg tcaaggttca tgttacatcc tcccgcaatt
    17281 aagagcgcga tatgataacc agaccgggtc ggtccaacaa cgtattaccc aaaattccag
    17341 taataagttc caaatattgc cgatatttta agcaaaatgc ttatgcatga ttattcattc
    17401 acgatattaa taatgtaact tatattttcg tgaaatctgt cactgaagaa aattggcaac
    17461 taaaggttaa aaccgttata acacagtcac cggcgcagag gagacaatgc cggatttaag
    17521 acgcggatgc actgctgtgt gtactgtaga gtctggcgga tgtcgacaga ctctattttt
    17581 ttatgcagtt ttaactttgc agatagccgc attctcgccg gaattgcgtg attaatgacg
    17641 cggtcagtgg tgtctggcga ctatcacgcc gctgaatcaa ataataggcc gctttcggta
    17701 aaatttctct aaccggcaac atcaccagcc cctgtccgtg caagggatcg cagcccattt
    17761 cttcaggcag tatgctgaga aaatcgcttt ttgccaccag actgatacag gctgagaacg
    17821 tctcgcagac tacaccgacc tgtggcgttt gcgcctgatc gtcaagcaat tcactcaact
    17881 gtttgtagta gctgccgtgt ggcgtcggca ttgtccagct gtaatccagt aactgtttga
    17941 tcgaacgggc accaatggcg gggtgtcccg ggcggcaaaa gatcgcgaat tgcttttcca
    18001 gtaatttctc aaaagtaaat tcgtggtcgt acggtccctg ataataggta ttgatggtga
    18061 aatccaattc tccctgacgc aattcattaa tcatcgacac cagttgccct tccataatgc
    18121 gtacttttac ctgcggatgc tgctgatgaa aacgagatat gacagctggc atcagactgc
    18181 gggaaatact ggcccccatg ccgatattaa tctgccctgc cagttgccct tgtcgttggc
    18241 gaatatcctc ttgggctgcg cgcagctctt caagaattag actggcgtgc tgataaaaac
    18301 tttcaccggc atcagttaac gtcacgcctt tactacggcg aaaaaagagt tgcgccgcta
    18361 acccttcttc tagctcctga atagatttac tcagtgccgg ttgcgacata ttcaacattc
    18421 ggctcgctcc gcgaatgctg ccctgacgag ccacttcaac aaaagcccga atttgatgaa
    18481 tttttacctg aaaagccatg acgccaccga taaccgttat ttatcagacc aaagaaactg
    18541 gcatctactt taatgcagat gattgtgtca gggtaattta tgaacggtta aaactgtgaa
    18601 aaatcagtta gtgataagta aaaactatcg ctacgtgaac cgggtcacac tttttactga
    18661 tgacgggaaa ggttatggag tctttgaatc aatttgttaa ttcgcttgcc ccaaaattat
    18721 cgcactggcg acgtgatttt catcactatg cagagtctgg ctgggtggaa ttccgcactg
    18781 ccacccttgt tgcggaagaa ttgcaccagc tcggctattc actggcgctg ggtcgcgaag
    18841 tagttaatga aagtagccgg atgggattac ctgatgaatt cactctacaa cgcgaattcg
    18901 agcgcgctcg tcaacagggt gcgctagcac aatggattgc ggcttttgaa ggtggtttca
    18961 ctggtatcgt cgccaccctg gataccggtc gccccggtcc ggtgatggct ttccgtgtcg
    19021 atatggacgc gctggatctc agtgaagagc aggatgtcag ccatcgcccc taccgcgacg
    19081 gttttgcgtc atgtaacgcc ggaatgatgc atgcctgtgg tcatgatggg cataccgcca
    19141 ttgggcttgg gctggcgcat acccttaaac agttcgagtc cggactacat ggcgtcatca
    19201 aactgatttt tcagcctgca gaggaaggta cgcgtggcgc gcgggcgatg gtcgatgcag
    19261 gtgtagtgga tgatgttgat tattttactg ccgtgcacat tggcactggc gtacctgcgg
    19321 gcaccgtggt gtgcggcagt gataatttta tggcaaccac caaatttgac gcgcacttta
    19381 ccgggaccgc cgctcacgca ggcgcaaaac cagaagacgg tcacaatgcc ttgttggcgg
    19441 cagcacaagc aactcttgca ctacatgcaa tcgccccgca cagcgaaggc gcttccagag
    19501 taaacgtggg cgttatgcag gcaggaagcg gtcgtaatgt tgttcctgcc tcggcgttgc
    19561 tgaaagtgga aacacgcggg gccagcgacg tcattaatca atatgttttt gaccgtgctc
    19621 agcaagcgat tcagggcgca gcaaccatgt atggtgtcgg cgttgaaact cgtctgatgg
    19681 gtgcagctac cgccagttct ccttcgccgc aatgggtcgc atggttgcaa agtcaggcgg
    19741 ctcaggtcgc gggggtcaat caggccattg aacgtgttga agcgcctgcg ggttccgaag
    19801 atgccacatt aatgatggcc cgcgtgcagc aacatcaagg gcaagcctcc tacatggtgt
    19861 ttggcacaca gctggcggca ggtcatcaca acgaaaaatt cgattttgac gagcaggttc
    19921 tcgctattgc cgtcgaaacg ctggcgcgca ccgcgctcaa ttttccctgg acgcgaggta
    19981 tctgatgcag gaaatctatc gttttatcga cgatgcgatt gaagccgatc gccaacgtta
    20041 taccgatatt gccgatcaaa tctgggatca tccagaaaca cgttttgaag agttctggtc
    20101 agcggagcat ctggcttcgg cgctggaatc tgcaggcttc accgttaccc gcaacgtagg
    20161 caatatccca aatgccttta ttgcttcgtt tggtcaaggc aaaccggtta tcgccctgct
    20221 gggagaatat gacgccctgg caggtttaag tcagcaagca ggttgcgcgc aacctacatc
    20281 cgtgacgccc ggtgaaaatg gtcacggttg cggacacaat ttgctgggaa ccgccgcctt
    20341 tgccgctgca atagccgtca agaaatggct ggaacaatat gggcaaggcg gcacggtgcg
    20401 cttttatggt tgtcctggcg aagaaggcgg ctcgggtaaa acgttcatgg ttcgcgaggg
    20461 ggtatttgat gatgtggatg cggcactcac ctggcacccg gaagcctttg ccggtatgtt
    20521 caatacccgt acgctggcaa acattcaggc atcatggcgc tttaaaggga tcgcagcaca
    20581 tgccgcgaat tcccctcatt tgggacgcag cgcccttgat gccgtaacgt tgatgaccac
    20641 tggcaccaac ttcctcaacg aacatattat tgaaaaagcg cgcgtacact atgccatcac
    20701 aaatagtggc gggatctcgc ccaacgtggt ccaggcgcag gcagaagtgc tttatcttat
    20761 ccgcgccccc gaaatgaccg acgtgcagca tatttatgat cgagtcgcca aaatcgccga
    20821 aggtgcggca ttgatgaccg aaaccacggt tgaatgccgc ttcgacaaag cctgttccag
    20881 ttatctcccg aatcgcacct tagaaaatgc catgtaccag gccctatccc attttggtac
    20941 cccggaatgg aactccgaag aactggcttt tgcgaaacaa attcaggcta cgctcacccc
    21001 caacgatcgg caaaacagtc tgaataatat cgccgcaacc ggtggcgaaa acggcaaggc
    21061 ttttgcacta cgtcatcgtg aaacggtact ggcgaatgaa gtcgctccat atgccgccac
    21121 cgataacgtg cttgcggcat cgactgatgt cggcgacgtc agttggaaac tgcctgttgc
    21181 ccagtgtttc agcccctgtt ttgccgtcgg tacaccgcta catacgtggc aactggttag
    21241 ccaggggcga acatctattg ctcataaagg aatgctgctg gcggcgaaaa ctatggcagc
    21301 aaccacactc aatctcttca ttgattcagg gctattgcaa gaatgccaac aagagcatca
    21361 gcaagttacg gacacgcaac cgtatcactg ccctatctcg aaaaacgtga caccgtcacc
    21421 tttaaaataa caacaacaac gcaaacacaa caaccgagga atgcccatga gtatgtcatc
    21481 cataccgtcg tcctcccaat ccgggaagct ctatggctgg gtcgaaagaa ttggtaacaa
    21541 ggttccccat ccttttttgc tctttatcta tttgattatc gtactcatgg tgacgacggc
    21601 aattttgtcg gcctttggcg tcagtgcgaa aaacccgacc gatggtacgc cggtggtggt
    21661 gaaaaacctg ctcagtgtgg aaggattaca ctggttttta cccaatgtta ttaaaaactt
    21721 tagcggtttt gctccacttg gtgcgatcct ggcgctggtt ttaggtgccg gtctggcgga
    21781 gcgcgtcggc ttactgccag cgctaatggt taaaatggca tcgcatgtta atgcccgcta
    21841 cgccagttat atggtgctgt ttattgcttt tttcagccac atttcttccg atgcggcgtt
    21901 agtgatcatg ccaccgatgg gtgcgctgat ttttctggcg gtgggcaggc atccagttgc
    21961 aggtttactg gctgccattg caggcgtagg ttgcggcttt acggctaatt tactgattgt
    22021 cacaaccgac gtgttgctgt cggggatcag cacggaagcg gcggctgcgt tcaatccgca
    22081 aatgcacgtc agtgtaattg ataactggta ttttatggcc agctccgtag tcgtactgac
    22141 gattgttggc ggcctgataa ccgacaaaat catcgagcca cggttaggtc aatggcaggg
    22201 aaacagcgat gagaaactgc agacattgac cgaaagtcag cgttttggtt tacgcatagc
    22261 aggtgtcgta tcgctacttt ttattgctgc gattgcgctg atggtgatcc cggaaaacgg
    22321 gatattgcgc gatccgatta atcacaccgt gatgccatca ccctttatta aaggtatcgt
    22381 gccactgatc attctttttt tctttgttgt ctcgctggct tatggcatcg ctacccgcac
    22441 aattcgacgt caggcggatt taccgcattt aatgattgaa ccgatgaaag agatggcggg
    22501 atttatcgtg atggtttttc ccctcgccca gtttgtcgcc atgtttaact ggagcaacat
    22561 ggggaaattc atcgccgtgg ggctgaccga tatactggaa agttcagggc ttagcggcat
    22621 cccggcgttt gtcggtctgg cgttgctttc ctctttctta tgcatgttta tcgccagcgg
    22681 ttccgcaatc tggtcgattc tggcccccat tttcgtacca atgtttatgc tacttggctt
    22741 tcacccggca tttgcgcaaa tcctctttcg tattgccgac tcatccgtat tgcctttagc
    22801 gccagtatct ccttttgttc cactgtttct tggattcctg caacgctaca aaccagacgc
    22861 gaaactgggt acttactatt cgttagtctt gccctatccg cttatctttt tggtggtatg
    22921 gctgctaatg ttgctggcgt ggtatcttgt gggcctgccg ataggtccgg gtatttaccc
    22981 acgtttgtct taagagagaa cggatgctga gattacttga agaaaaaatt gccacgccac
    23041 tgggtccact gtgggtgatt tgcgatgagc aatttcgcct gcgggcggtt gaatgggaag
    23101 agtacagcga acgcatggtg cagctgctgg acatccatta tcgcaaagaa ggctatgagc
    23161 gcatttctgc caccaatcca ggcggtttaa gcgacaagct tcgtgaatat tttgccggta
    23221 atcttagcat tattgatacg cttcccactg ctacgggggg gacgccattt cagcgcgaag
    23281 tctggaaaac actacgcact atcccctgcg ggcaggtaat gcattacggc gaactggctg
    23341 agcaattggg ccgtcctggc gcggcgcgtg ccgttggtgc ggcaaacgga tcgaatccca
    23401 tcagcatcgt cgtaccttgc catcgggtta ttggccgaaa cggcaccatg accggatatg
    23461 caggcggagt tcagcgaaaa gagtggttat tgcgccacga aggttatctt ttgctgtaaa
    23521 cattaaacaa tttgtgccag cttgttcaca cttttatgta aagttaccct taacaactta
    23581 agggttttca aatagataga catatattta catctaatat cggaattctc tgctgttaag
    23641 gtttgcttag acttacttgc tccctaaaaa gatgttaaaa ttgacaaata tcaattacgg
    23701 cttgagcaga cctatgatcc cggaaaagcg aattatacgg cgcattcagt ctggcggttg
    23761 tgctatccat tgccaggatt gcagcatcag ccagctttgc atcccgttca cactcaacga
    23821 acatgagctt gatcagcttg ataatatcat tgagcggaag aagcctattc agaaaggcca
    23881 gacgctgttt aaggctggtg atgaacttaa atcgctttat gccatccgct ccggtacgat
    23941 taaaagttat accatcactg agcaaggcga cgagcaaatc actggtttcc atttagcagg
    24001 cgacctggtg ggatttgacg ccatcggcag cggccatcac ccgagcttcg cgcaggcgct
    24061 ggaaacctcg atggtatgtg aaatcccgtt cgaaacgctg gacgatttgt ccggtaaaat
    24121 gccgaatctg cgtcagcaga tgatgcgtct gatgagcggt gaaatcaaag gcgatcagga
    24181 catgatcctg ctgttgtcga agaaaaatgc cgaggaacgt ctggctgcat tcatctacaa
    24241 cctgtcccgt cgttttgccc aacgcggctt ctcccctcgt gaattccgcc tgacgatgac
    24301 tcgtggcgat atcggtaact atctgggcct gacggtagaa accatcagcc gtctgctggg
    24361 tcgcttccag aaaagcggta tgctggcagt caaaggtaaa tacatcacca tcgaaaataa
    24421 cgatgcgctg gcccagcttg ctggtcatac gcgtaacgtt gcctgatttt tccgcataac
    24481 tcactatcct tctgtcatat cattaaattt ttctgattta ttgatctggc agaaggttca
    24541 tcactgtttc attcaccaga tatgggttaa tcttttaatt acaaactgcg ttgacagttg
    24601 ttgtaaggag accctgtatg gctatgtatc agaacatgct cgttgttatc gatcctaacc
    24661 aggacgacca accagcattg cggcgagctg tttatttaca tcaacggatt ggtggcaaaa
    24721 ttaaagcctt tttgccgatc tatgacttct catacgaaat gaccaccctg ctctccccgg
    24781 acgaacgtac cgctatgcgt cagggcgtca tcagccagcg tacagcctgg atccacgagc
    24841 aggcaaaata ttatctcaat gctggcgttc ccattgaaat taaagtggtc tggcataacc
    24901 gtcctttcga agccatcatt caggaagtga tcagcggcgg gcacgatttg gtgctaaaaa
    24961 tggcgcacca acatgaccgt ctggaagcgg tgatttttac gccaacggac tggcatctgt
    25021 tacgcaaatg cccaagcccg gtgtggatgg tgaaagacca gccgtggccg gaaggaggta
    25081 aggcgctggt ggcggtgaat ctcgccagtg aagagccgta tcataatgcg ctcaatgaaa
    25141 aactggtcaa agagacgatc gaactggcag aacaagtcaa ccataccgaa gttcatctgg
    25201 ttggcgctta cccagtaacg ccaattaata tcgcgattga actgccggaa tttgatccga
    25261 gcgtttataa cgatgccatt cgtgggcaac atttgctggc aatgaaagct ctgcggcaga
    25321 aattcggcat taatgaaaac atgacgcacg tagaaaaagg tctgccagaa gaggtgattc
    25381 ctgatttggc ggagcactta caggcgggga ttgtggttct gggcacggta ggacgcaccg
    25441 gtatttcagc agcattcctc ggcaacacgg cggaacaggt gattgatcat cttcgctgcg
    25501 atctgttggt cattaaacct gaccagtatc agacacccgt tgaactggat gacgaagaag
    25561 acgattaacg actcttcaat aactgacaag gcccgagtta tcgggcctat tttttactta
    25621 ctgctcttcg ccgccaagga aataaattcc caacgggatt gccagcaaca cagtaaagac
    25681 caggctataa acaaaaatca tcgatgactg aatcacatac attgatgtcg tccactcaga
    25741 cagcggaata ttgtactgtt ctataacgcc gccgatagtc gcgcgagtca caaccgacgc
    25801 cgcaataatg aaaaccgcca acaaaccaaa gagaaatttc ttccctttcg ctgacttcag
    25861 tttcgccata atcatgatgt aatccttgtg cagacaaatt tcttgttacc taaacgtaac
    25921 aataccatga catttcaccg ttgtctgctc tgaaaacctc acctcttagc tggatttaca
    25981 atcaacgcat tgattagtta gctaatgttg ctacttcctt ccctgactat tgatgatctg
    26041 aatgaattgt cgcggtaaaa tagccccgcc aggggtttcc ggcatgacga caattatctg
    26101 gaggagcctg ataaaacaat gatcgctgaa ctgtttacaa ataatgcgct taatctggtc
    26161 attattttcg gtagctgcgc agcattgatt ctgatgagct tttggtttcg ccgcggaaat
    26221 cgtaaaagaa aaggcttttt attccatgcg gtgcaatttt taatctacac cataattatc
    26281 agtgctgttg gtagcattat taattatgtt atagaaaact ataaactcaa atttatcacc
    26341 ccaggcgtta tcgattttat ctgtacgtcc ctgattgcgg ttattctgac gattaagcta
    26401 tttttgctga ttaatcagtt tgaaaaacag cagattaaaa aaggtcgcga tatcaccagt
    26461 gcgcggatta tgtcgcgcat tatcaaaatc accattattg tggtgcttgt tctgctttat
    26521 ggcgaacatt tcggcatgag cctttctggc ttgctgacct ttggtggtat tggtggtctg
    26581 gctgtcggta tggccggtaa agatattctg agtaatttct tttccgggat catgctctat
    26641 ttcgaccgtc ctttcagtat tggcgactgg atccgttcac cggacagaaa tatcgaaggt
    26701 acagtagcgg aaattggctg gcgaattacc aaaattacga cctttgataa tcgtccattg
    26761 tacgtaccga actcgctgtt ttcgtcgatc agcgtagaaa acccaggacg aatgaccaac
    26821 cgccgcatta ccacgaccat tggtttacgt tatgaggatg cggcaaaagt gggcgttatc
    26881 gtcgaagccg tacgtgagat gctgaaaaat cacccggcca tcgaccagcg acaaacctta
    26941 ctggtttatt tcaaccagtt tgctgactcg tcattgaata ttatggttta ttgctttacc
    27001 aaaaccacgg tatgggctga gtggcttgct gcacagcaag acgtttattt gaagattatc
    27061 gatattgtac agtcacacgg cgcggacttt gccttcccca gccagacgct gtatatggat
    27121 aacattacac cgccggaaca gggtcgctaa atcagacatc actggcgtta tccacaaggg
    27181 taacgccagt tcccatcaca tcaatgcttc acaatataca tagtccgact gtacgccaca
    27241 tcttcagggt tattaatggg ataacctttc agccacggtt tgattaatcg tccattggta
    27301 tattgataaa ttggtgcaat cggtgcttgc tccatgagga ttttttctgc cgcgttgtaa
    27361 tcggcattac gcgctttaac ggtattttcc gtcgatgcct gggccagaac tttgtcgtat
    27421 gccggattgt taaagcgtga aatatttcct gaatgcgttg acgttaataa tgtcaggaaa
    27481 gtggagggtt cattataatc ccccacccat gaggcgcgga taacatcaaa attgccggtg
    27541 ttacggctat cgatataggt tttccattcc tgattttgca atttaacatc tacgccaagg
    27601 ttctttttcc acatcgatgc tacagcaatc gcaatttttt gatggttttc tgaagtgtta
    27661 tacaaaagcg tcagcttcag cggtttttgc ggaccataac cagctgcgct caacaaagtt
    27721 tttgcctgcg cattcagttc ttcctgactc atttgttcaa acggcgaagg ttccggcgta
    27781 aatccggcgg taacatctgg tgtaaaatgc cacgctggct tttcgcccgt ccctaatact
    27841 ttttcggtca tcaggcggcg atctatcgtc atacttaatg ccagacgaac gcgctgatct
    27901 gccgtcggac ctttttgcgt gttaaacgca taataatagg tcccgagctg cggcggcgta
    27961 taaacctgcc ccggaatatc cttcaacagc ttctgataca tatttttcgg gaaggattcg
    28021 gtgatatcaa tatcccccgc gaggtaacgc ttagtggctg cggattcctg attaattggc
    28081 aggaaggtca ctttttgcag taccgttttg gcgttatccc aataatgggt attcggtacc
    28141 acgaccagtt tttcattgac tacgcgttct ttaagaacat aagcgccatt gccgatcaga
    28201 tttccggatt tcgtccactc tttaccgctt tctacgttgg ctttttgcac cgggaagaag
    28261 gcaaagttag cggttaaatt cacaaaccac ggcaacggtt tatcaagctg aattttcaaa
    28321 gtatgggcat caactgcggt gacgccaagc tggtcaggcg tagctttacc atcaataatc
    28381 gcctgtgcgt tgttgattcc cgccagcgcg gcaaaccatg caaatggcga tagcgttttc
    28441 gggtccacca gacgctgcca gctgtagaca aaatcttgcg ccgttaccgg tgtgccatcc
    28501 gcccattttg cgttatcgcg cagggtaaaa gtccagatac ggttgtcatt acttttccac
    28561 tgagtcgcaa cgccggggac aatttcccct ttctcgttct gattcaccag tccttcaaac
    28621 agatcgcgaa tgacctgaat ctctggcagg cccacggctt tagcgggatc cagcgacgca
    28681 ggctcatctt taatgtggcg caccagctcc tgcttctctg ccagtactgt gccgctcgga
    28741 acttctgcag catacgaaag agaaatgctg ctgaccaaca gcgcacaaca cgtgactgaa
    28801 acagagtgct tcataagttt ccctctaacc gacattaaaa ttgatgcgta attatttgtt
    28861 tcgccttaaa gaaatgcaaa caacttccat caaaaaagtg acacaggaag cataaaatgt
    28921 tttatgtatc aatatattaa atca
//
LOCUS       DAZFKF010000059        28872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFKF010000059 DAZFKF010000000
VERSION     DAZFKF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.059"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..606
                     /locus_tag="ACHKA1_003056"
     CDS             <1..606
                     /locus_tag="ACHKA1_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFO5680675.1"
                     /translation="QQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTIN
                     LSPGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLC
                     EPDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGT
                     PWVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            587..2890
                     /gene="trbC"
                     /locus_tag="ACHKA1_003057"
     CDS             587..2890
                     /gene="trbC"
                     /locus_tag="ACHKA1_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFO5680676.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEVIQSGDKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            3357..3587
                     /locus_tag="ACHKA1_003058"
     CDS             3357..3587
                     /locus_tag="ACHKA1_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680677.1"
                     /translation="MKTNMGSTKNGARVPHVRNRFTSMDIGMNSQILPDMLINFALIN
                     ITDRKNEGTNTIDGNWQADEGRRYRDNVRIYF"
     gene            complement(3685..4116)
                     /locus_tag="ACHKA1_003059"
     CDS             complement(3685..4116)
                     /locus_tag="ACHKA1_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFO5680678.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(4184..6895)
                     /locus_tag="ACHKA1_003060"
     CDS             complement(4184..6895)
                     /locus_tag="ACHKA1_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO5680679.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDMQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPVSGHINRLSWSQERLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYEP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(6907..7239)
                     /gene="mobA"
                     /locus_tag="ACHKA1_003061"
     CDS             complement(6907..7239)
                     /gene="mobA"
                     /locus_tag="ACHKA1_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO5680680.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            7472..7807
                     /locus_tag="ACHKA1_003062"
     CDS             7472..7807
                     /locus_tag="ACHKA1_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO5680681.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKRAPKKTDSGR
                     NDGIDMNNN"
     gene            complement(7892..8740)
                     /locus_tag="ACHKA1_003063"
     CDS             complement(7892..8740)
                     /locus_tag="ACHKA1_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5680682.1"
                     /translation="MNQTLPTADLNTDGTTDVIPSVAIDRIIAQRNEGISLFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEHGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGELFEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            9331..9582
                     /locus_tag="ACHKA1_003064"
     CDS             9331..9582
                     /locus_tag="ACHKA1_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO5680683.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(9613..9807)
                     /locus_tag="ACHKA1_003065"
     CDS             complement(9613..9807)
                     /locus_tag="ACHKA1_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680684.1"
                     /translation="MTIAERLIQKGFDEGFKQGFKQGALEVAREIACRLRDIGWPPER
                     IQEATGLSGEELKKLFPDEQ"
     gene            complement(9869..10831)
                     /locus_tag="ACHKA1_003066"
     CDS             complement(9869..10831)
                     /locus_tag="ACHKA1_003066"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO5680685.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPASFVEPDLHQYASDILWSVKTTGGKDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQYKTLPLVIPVLFYHGERSPYPYSMNWLDCFEDPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQQGIQKGIQQGIQQGIQQGIQQGIQ
                     QGIQKGRMEEKQDIARQLQKTGMPSAQICQITGLSEAELKNITH"
     gene            complement(10828..11217)
                     /locus_tag="ACHKA1_003067"
     CDS             complement(10828..11217)
                     /locus_tag="ACHKA1_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFO5680686.1"
                     /translation="MKSCFDTDKDFQMSSDEVTFNADSVRSKLELLSEELMHRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDAERGYCCHG
                     FDRNCCPLGCGDLDDDCCDAADLMEEP"
     gene            complement(11407..11673)
                     /locus_tag="ACHKA1_003068"
     CDS             complement(11407..11673)
                     /locus_tag="ACHKA1_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680687.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSERKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(11766..12200)
                     /locus_tag="ACHKA1_003069"
     CDS             complement(11766..12200)
                     /locus_tag="ACHKA1_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFO5680688.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            complement(12930..13430)
                     /locus_tag="ACHKA1_003070"
     CDS             complement(12930..13430)
                     /locus_tag="ACHKA1_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO5680689.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(13891..14487)
                     /locus_tag="ACHKA1_003071"
     CDS             complement(13891..14487)
                     /locus_tag="ACHKA1_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680690.1"
                     /translation="MMKSDEKYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLAWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVRAAQDWLCDRKLVDITDILR"
     gene            complement(14484..15203)
                     /locus_tag="ACHKA1_003072"
     CDS             complement(14484..15203)
                     /locus_tag="ACHKA1_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO5680691.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGELCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENTI"
     gene            complement(15200..15634)
                     /gene="psiB"
                     /locus_tag="ACHKA1_003073"
     CDS             complement(15200..15634)
                     /gene="psiB"
                     /locus_tag="ACHKA1_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO5680692.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(15689..17647)
                     /locus_tag="ACHKA1_003074"
     CDS             complement(15689..17647)
                     /locus_tag="ACHKA1_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO5680693.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEDVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGSCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCTPRPQMDATEHTTNLADAA"
     gene            complement(17711..17944)
                     /locus_tag="ACHKA1_003075"
     CDS             complement(17711..17944)
                     /locus_tag="ACHKA1_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO5680694.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDNGEHFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            complement(18002..18529)
                     /locus_tag="ACHKA1_003076"
     CDS             complement(18002..18529)
                     /locus_tag="ACHKA1_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5680695.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     KTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPLQNAQAQPQPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            18881..18976
                     /locus_tag="ACHKA1_003077"
                     /pseudo
     CDS             18881..18976
                     /locus_tag="ACHKA1_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18976..19277)
                     /locus_tag="ACHKA1_003078"
                     /pseudo
     CDS             complement(18976..19277)
                     /locus_tag="ACHKA1_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19291..19482)
                     /locus_tag="ACHKA1_003079"
     CDS             complement(19291..19482)
                     /locus_tag="ACHKA1_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680696.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(19482..19901)
                     /locus_tag="ACHKA1_003080"
     CDS             complement(19482..19901)
                     /locus_tag="ACHKA1_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO5680697.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIARDWNLSD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            complement(19948..20373)
                     /locus_tag="ACHKA1_003081"
     CDS             complement(19948..20373)
                     /locus_tag="ACHKA1_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5680698.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            complement(20594..20833)
                     /locus_tag="ACHKA1_003082"
     CDS             complement(20594..20833)
                     /locus_tag="ACHKA1_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680699.1"
                     /translation="MKSAAWQMHWRKSGNDDAGGEKSPPFPAGPRTEQKDVAAGSALP
                     GHAGHGLVGFQLCGLSVVCGLCRASILAGLAGMQG"
     gene            complement(20787..21557)
                     /locus_tag="ACHKA1_003083"
     CDS             complement(20787..21557)
                     /locus_tag="ACHKA1_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680700.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDIEQLNPHYPGWISIYVR
                     LDVSRLATLLISRHDGVLPPVLASAMQKLTGTVAELVLSGSQWQALPVLPADGTQVSF
                     PYAGEWLTEEEIRAVLDAVRNAVRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVCECVEHILASGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMADALAKIRK"
     gene            complement(21602..22088)
                     /locus_tag="ACHKA1_003084"
                     /pseudo
     CDS             complement(21602..22088)
                     /locus_tag="ACHKA1_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(22048..22269)
                     /locus_tag="ACHKA1_003085"
     CDS             complement(22048..22269)
                     /locus_tag="ACHKA1_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680701.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(22270..22881)
                     /locus_tag="ACHKA1_003086"
                     /pseudo
     CDS             complement(22270..22881)
                     /locus_tag="ACHKA1_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            23503..25203
                     /gene="ltrA"
                     /locus_tag="ACHKA1_003087"
     CDS             23503..25203
                     /gene="ltrA"
                     /locus_tag="ACHKA1_003087"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFO5680702.1"
                     /translation="MTAKNINAGAPTDIVTHWHNINWAKCRREVRRLQARIVKAIQEE
                     KAGKVKALQWLLTHSFSARAMAVKRVTDNRGKRTPGVDGKTWSKPGSKMKAIYTLKRR
                     GYKPLPLRRIYIPKSNGKKRPLGIPTMKDRAMQALYLMALEPVAETTADPNSFGFRPC
                     RSTADAIEQCFTTLHRADRAQWILEADIRSCFDEISHEWLIANIPTDTAILKRWLKAG
                     YIDLGKLYPTSAGTPQGGIISPTLANMVLDGLQPLLKKTFYRGGLNPEKINIIRYADD
                     FVITGISHDTLSEKVLPLLENFLAERGLTLSPEKTRITHISDGFDFLGMNIRKYNGKL
                     LIKPSPQKVKEFLKRVRQTIKDNPTVSQSTLIWHLNPVLKGWVRYYRHVVSKKIFQKV
                     SHEIWKALWRWACRRHPKKGTGWIRKKYFRSVNFRHWVFGTETGKLLPSGKRELLALY
                     EISDMPVKRHRQIKSTANPFDPSYESYFEARLKATMLESIQGYSRLRNLWLRQGGQCP
                     VCHLPVNATTGWQLRHIVPKSEGGLDNIGNLAMLHPACHHTAKHQGISVMKPALMWSY
                     "
     gene            complement(25285..25356)
                     /locus_tag="ACHKA1_003088"
                     /pseudo
     CDS             complement(25285..25356)
                     /locus_tag="ACHKA1_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25432..25743)
                     /locus_tag="ACHKA1_003089"
     CDS             complement(25432..25743)
                     /locus_tag="ACHKA1_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680703.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLPSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(25740..26642)
                     /locus_tag="ACHKA1_003090"
     CDS             complement(25740..26642)
                     /locus_tag="ACHKA1_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO5680704.1"
                     /translation="MYFSAEPAQITEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIKVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            27060..27308
                     /locus_tag="ACHKA1_003091"
     CDS             27060..27308
                     /locus_tag="ACHKA1_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO5680705.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSLSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            27305..27742
                     /gene="umuD"
                     /locus_tag="ACHKA1_003092"
     CDS             27305..27742
                     /gene="umuD"
                     /locus_tag="ACHKA1_003092"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO5680706.1"
                     /translation="MSTVYHRPAAPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLHPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            27742..>28872
                     /gene="umuC"
                     /locus_tag="ACHKA1_003093"
     CDS             27742..>28872
                     /gene="umuC"
                     /locus_tag="ACHKA1_003093"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HFO5680707.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAIEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRTLKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKT"
BASE COUNT         6853 a         8213 c         7402 g         6404 t
ORIGIN      
        1 cagcaggaac cttcttctgc ccccgccccg acggcagtga cggaagaaca acacgttcag
       61 gccgctgagg cccgccgtaa tctggccaca gtacttaaac gcaacgcaga ccgggggatg
      121 ttcaccatta acctgtcccc tggtcatgaa cgcacgctgt acgccttcct tgaccccgca
      181 tgcatcaact gccgtctgct ggaacccgcg cttaagcaac tggcccggga attcagcgtg
      241 gtgatttatc cggtctccgt gattggcggg gagtccagta caaagcggac tgctccgctg
      301 ctgtgtgaac cggactcaca aaaacgggcg accggatggc atcagctgta ttccgctgac
      361 agtggtatgg cccccaccgg cgatgcaccg gccgcacagg atggacagtg tctgaccgcc
      421 gcacgtgctg ccatcgatgt caataaccag gctttccgcc agttcggttt tggcggaacc
      481 ccctgggtgt tatccgatac cggctggcac ctgccgtccg gcatcctgaa agacgccact
      541 tccgttcgcc tcttcctgaa agcaacagat agcgaggagt atcacaatga gccagcacca
      601 cgttaatcct gacctcatac accgcacggc ctggggaaac ccgttgtgga atgcactgca
      661 taacctgaac atcactggtc tgtgtctggc cggcagtatc attacggcgc tgatatggcc
      721 actggccctt cctgtctgcc tgctgttcac cctggtcacc tccgtgatat tcatgctgca
      781 acgctggcga tgccccctgc gtatgcccat gacactcagc cttgatgacc cctcccagga
      841 cagaaaggtc aggagaagcc tcttcagctt ctggcccacc ctgtttcagt atgaagcgga
      901 tgaaacattc cccgcccggg ggatttttta tgtcggttac cgccgtatca acgatatcgg
      961 tcgtgagctc tggctgagca tggatgacct cacccggcat gtcatgtttt ttgccaccac
     1021 cggtggcggt aaaacagaaa ccacctttgc ctggctgctc aacccgctct gctggggaag
     1081 aggctttacc tttgttgacg gcaaggcaca gaacgatacc acgaggacaa tctggtatct
     1141 ctcccgacgt tttggcaggg aggatgatat cgaagtcatc aacttcatga acggcggaaa
     1201 atcccgcagt gaagtcattc agagtggcga taaatcccgc ccacagtcaa acacctggaa
     1261 cccgttcgca ttcagcaccg aagccttcac cgccgaaacc atgcagtcca tgctgcccca
     1321 gaacgttcag ggcggtgaat ggcagtcacg ggcgatagcc atgaacaagg cactggtctt
     1381 tggcacaaaa ttctggtgcg tccgggaagg taaaaccatg tccctgcaga tgttacggga
     1441 acatatgacg ctggaaggga tggcaaaact ctattgccgt ggccttgatg accagtggcc
     1501 ggaagaagcc atcgcaccac tgcgaaacta cctgcaggat gtcccgggat tcgatatgtc
     1561 actggtgaga acgccgtcag cctggactga ggagccccgt aaacagcatg cttatctctc
     1621 cggtcagttc tcggaaacgt tcacaacctt tgccgaaacc ttcggggacg tctttgcggc
     1681 agatgccggc gacatcgata ttcgcgacag tattcacagc gaccgcattc tgattgtgat
     1741 gatccctgca ctggacacat cggctcatac cacgtcggca ctcggtcgta tgttcgtcac
     1801 ccagcagagc atgctcctcg cccgtgacct gggctaccgg ctggaaggca cagatgcaca
     1861 gaccctggaa gtcaaaaaat acaaaggcag cttcccctat atctgtatcc ttgacgaagt
     1921 cggtgcctac tacacagacc gcattgctgt ggaggccacc caggtacgtt cactggagtt
     1981 ttccctgata atgacggcgc aggatcagga gcgaattgaa gggcagacct ccgccaccaa
     2041 caccgccaca ctgatgcaga acgccggcac caaattcgcc ggacgcattg tcagtgacga
     2101 caaaacggca agaacggtga aaaatgccgc cggtgaagag gcacgggcca gaatgggcag
     2161 cctgcagcgc cacgatggtg tgatgggtga gtcctgggtg gacggtaacc agatcaccat
     2221 ccagatggaa agcaaaatcg acgtgcagga tttgatcaga ctgaatgctg gtgagttctt
     2281 taccgttttc cagggggatg tggtgccgtc agcctctgtg tatattccgg acagcgagaa
     2341 aagttgcgac agtgatccgg tggtcatcaa ccgctatatt tccgttgaag ccccccggct
     2401 ggagcgtctc cgccgtctcg tccccaggac agtgcagcgc aggctcccca caccggagca
     2461 tgtcagcagc atcatcggtg tactgacagc aaaaccatcc cgtaaacgtc gtaaaaaccg
     2521 tacagagcct taccgtatca ttgatacatt ccagcgccag ttggcaacag cgcagacatc
     2581 actggatctg cttccgcaat atgatacaga tatcgaatcc cgggcaaacg aactgtggaa
     2641 aaaggctgtg cacactatca ataacacaac cagggaagaa cgccgggtat gttatatcac
     2701 actgaatcgt ccggaagagg aacactccgg tcctgaagat ataccttcag tgaaggcgat
     2761 tctgaatact ctgctccccc tggagatgct gttacctgta ccggatctaa ctgcatcccc
     2821 gccccataaa aaaaatgtcg ctcagacacc atcaggtgga aaacaagaga gtcgtaaaaa
     2881 aagattctga taacacctgt atggctgatt ttaataacaa cctttacgcg attattaatc
     2941 tcctccgctc accatagtaa gcggaggatt caggatttca atccatcacc agaggtacaa
     3001 taaatgtgta ttagtagccc ggaaacaaac tcatggtctg ttatttatcg taaaaattcc
     3061 ggagaagata tcaatatcac ttccctgacg tttaaaaata gtcttctggc agcccggaca
     3121 ctgatggtgc ccgaaaacta tatgatatgc atactcagaa acggggagag ggtcagacga
     3181 tgggacagag aaatacttgc aggctcaaac agatggtaca agtgttctcc cgataatttc
     3241 gaaatactgg gtaagctccc cataataaat aaagtcacaa aactgataaa atcataatct
     3301 acggatttta atcagttaag ctaaatcagg atgaacgctt tgattatgaa ttatacatga
     3361 aaacaaatat gggcagcaca aaaaatggtg ccagggttcc ccacgttcgt aatagattca
     3421 cttctatgga tattggtatg aattcccaga tcctgccaga catgctgatt aattttgccc
     3481 ttattaacat cacagacaga aagaacgagg gtaccaatac cattgatggt aactggcagg
     3541 ccgatgaagg acgccgttac cgggataatg taagaatcta tttttgagta tgtcaacata
     3601 acctgaggca aagtacctca ggttccagtg tagtaatcag ccaacctgac agtcttctgt
     3661 tagccctaca gatatgtcgt ggagctaacc gcgcatgtca aagccggatt tttgaaaatc
     3721 tctataaact tccggattgt tgaaggccgg gaatttagct ttatgggctg ctgactttat
     3781 cgcttcgcaa taggctttat cgccattact ggttgatatt tttaacgccg tgccatcccg
     3841 ggagaattcc atatgcaacc tgcatttttt ccttttccat ttttgcggat catcaagttt
     3901 agcattaatt gcatccctga ttccccgtgc ttgctccccc cattcatcct gatcatccca
     3961 acgtcctgaa ctgcaactac ccagggctgt agttttatgg caatttgaag gatgtaatgg
     4021 cgcacatccc gccacaaagc tgactaaaat agccagcata acgattttct ttacccccat
     4081 atattgctcc tcaatccata tagatctcag taccatagct ctgtcagagc aaagaaaatc
     4141 ttaactaata caagttaaga tttttaatat ccactcctca tccctacatt ccaggtgatg
     4201 gtggcgtcca ctcattatcc tgaactggag cataagatgc gccatgccct ccggatatgc
     4261 ttttctgttg cctgatatat tcgtggtagt actgttcact acgctgacga tgtaaatcca
     4321 catccgggcg gctgattaca aagtgtccat ttccggttat tccagctgca ttatgccagg
     4381 ccaccatttc cgcaaataca tcattaaact gttgatatga gccttccggt tcaaaaccca
     4441 ttcctggctc acgatcaaac aatacaggag caatcagatt cagcttatcc accagttcat
     4501 ttctgtcctg atgatggtag aacaccacac ggtccccata atccacacag actaactcgc
     4561 cattcctccg atcacgaaaa cgcacactgc catctttctg cagacgggct tcgagaacgc
     4621 cagtgtcttc ataaagtgga gttcctcccg gttcacagag aatcacgcag cggtcggcat
     4681 tcgttgttcg tcgcttgtct ttgcgacgat cacgataatc ccagccacgt aactgtgata
     4741 atgcagctct gtctccctgt accgcctgtt gctccaccca ctgccggtag gacaaaggat
     4801 accacttacc ctccgcaatc agtgacaacc gctcttcctt cacactctcc ttcagcctga
     4861 tcagcgcctg catacgctgc acttctgcaa tatggtaatg cagcttacgc aattgtggat
     4921 cccggaactg cacacggata tacgccttgc gccgacggca ggcagcatga atttcccgta
     4981 accgttctcc gtaacgtaag tcgggtttac gccagtgctc cttccatgca agataacgcg
     5041 cgcgcaaatc ttcccgcgca gctgcacggg cttcacggcg ttcacgacgc agtacaggat
     5101 cgcgccggaa gccgggctcc acttcatcgc tggcagcaag ttccggattg tagcgacatt
     5161 caggcttcac ccgttcaaat atatctgcac ctgcaatctc aaatggccct gcctgcggct
     5221 cagccctgga taacgtcaaa tccgggtgga tactgctggc cttcactggt gtgagctcat
     5281 gattaaacgc atccacaatc acaagaccat gatgctgttt ctgcaacata agccctttac
     5341 gcgcaaacat ctcatggcac gcctgccagc tgaaattccc gcccgcgatc atatcttcaa
     5401 tcaacgactc acactgaaca gtcgtgtacc cccacagact ttccttatca gaaaacagtt
     5461 cgcgctttat tgctccctgt ggacctgtcc ccatccgcat acgataccat tcgtaatcac
     5521 tgacagcctg agtaccaaga ttacgtcccc ggaatcgttc tccgggtata acctgagtaa
     5581 aaatatcctt cggtacaggc tgccatccac catccagatc ctgcatataa tccagcgtca
     5641 ttaacggcca cacactgtct gcacgtactg gtgtctgttg tttatcaaat ccatcatgca
     5701 gtaccagatg cccgtgctgg atccccatcc ataatccagc ctttgccaga gttctgtgca
     5761 catccaggca actttccagc tcaccatttc tcgccatttc cggtaagcgt tcagcaaagt
     5821 gacgacaggc gtactgtttc agactctccg tttctgccac tttttccggc cggatatcca
     5881 cgttaatggc ttccggctca taacgaccgg gtgtccccac ctgactgaaa atatctgtcg
     5941 gtacaggctg atattccccc agtttccgcc ccagtttttc agctgtccat gacggtccga
     6001 acgaactgag tgcaacacct tcccgcgctc tgtcccagcc atccttcacc accagttccc
     6061 cttcctgcat cgtgatatac agaccatcac tggccagccg tttatgcaaa gataaccagt
     6121 cctgtgcagg ctcttcacgc agcccggcaa tcgacatctg agcaatatat tccctgaacg
     6181 tctgcttttt cccgcgtcgc caggcattac ggcgctcacg taccagcgct gttttgcgca
     6241 caatacggtt gccgggtgca tgcacaaagc agccattatc aggcgcgaat ccgtgcttaa
     6301 gttcaagttc acgacatgca cggctgagcc tttcctgact ccaggaaaga cgattaatat
     6361 gaccactaac aggatgaaca cgattcactg cgacatgtac atgcaggtta tccgtgtccg
     6421 tatgaacggc agccacgtac tgatgttttg agagttcaag ggatttcaat gtgtgacgca
     6481 cacagtcaaa aagctgctca ggacgcgggc tttcatgcgc aggccaggag agaatgtagt
     6541 ggaaaacggg gtccgtatca ttacgggaaa aaacagcttt gtccgccttg tactgcatct
     6601 cttcagcagc tgtttccagt gaattgcagt tatggaaaca ggtaacgcca tagaaattta
     6661 cccactgact gccgtctttc atcacgtcaa tcagggagac aaatttttcg ttacgcaggc
     6721 gtgtagcata gtcgacaagg cgtctgaaac ggttgcggtg agggacgtca ctcttgattt
     6781 cgctgtcttc tgtgagctca ttttcctgca tatcatcccg cacagatgtg tacgcaatca
     6841 ggtcttcaaa cgaagacctg ccgtcacgtc ttttgtgcgg aataatggca ttcatcagca
     6901 gttatctcag cgtcccatta agtctcttcg cagagcattg acatattcag taatggcaac
     6961 aagaacctcg gcatactcct tatcaccagt acgttttcct tccacaaaca gatgcttctg
     7021 aaggcggcca agacgcataa tctcatcaat catacggtcg tcagtacggc agttaaccac
     7081 cttatccagt gaagagcttc gtataaatgt tgaaaccgtc tgtccacttg actccgcttt
     7141 ttctctgacg atttcagcct catcctccgt gaatctcgcc gtgaatttta caattctctt
     7201 cctgttctca ctgcccgttc gggttttctt ctcactcatg gtctgctcct taccggggtg
     7261 tcggggcgaa gccctgacca ggaggcattt gtctgattgc gcgtgcgcgg tccgacaaat
     7321 gcacatcctg tcccgttttt caggacatta tagcctgtta tttttgtgca tcatagtctt
     7381 atgcagacgg cagaggcagg gcgcagccct gccaggagtc agtttttgat gataaaatca
     7441 ggcttcaaca tgaaacagag agaaagtgcc aatggcttct aaaaaatttt actcagatga
     7501 tgacatccag ctggcaaaag ctgctttatc ggaattgcca gatctcaccg ctcaacggaa
     7561 aacattgcgt gattttcttg atgccattcg tgatgacatt attgtcctgg taaggacaaa
     7621 gggttataca ctggcagatg ttcgtgacac gctcaaaaat gcagggtatg aagtaggaga
     7681 gaaggcactt cgtgacatta ttcgggaagc agaatcgaaa aaaccatccc gccgttcttc
     7741 ctctaaaaga gcacctaaaa agacagattc aggcaggaat gatggcatcg atatgaacaa
     7801 caattgatgt gacaatgtca catcgtgttc tgagattatg atgattacag aaaaaaagcc
     7861 ccggcataaa taccagggct tttctgttta atcagactcg tggcggcaat gcaccaggat
     7921 aatgccgtgc aataatatca ttcagttttt caacaaggtc aggacgctta aagacaatgt
     7981 gtacggtccc tttctggtag ccacgcaccg taaaatattc cccttcaaac aactcaccga
     8041 cattgtcgcc gttcccgaga aattcaccat actgcgcccc gatgctgttc ctgaagtcag
     8101 gaacgttgcg cccctccagc agataaaacg gacgtaccag gtcatccagt gcctcccgtc
     8161 cgctgcagtc cagcgtcaca cgcccgttac tccagcggta cagcaggttt tcaataatga
     8221 ttttctttcc cagacggcag ggattattgg ttctgtaatc ccatgacaat ttccggtaga
     8281 catcaatcag tccatgttca aatgtctgca tcttactggc attcagatgg cgaaaagttg
     8341 ccagtacatt gtccagggtg atttcaggac aggtgtcgct catcagctgg ctgttccact
     8401 catcacgctg gcatgaactc atgaatgtgt acatgccagt atccgtcatc aggcgatccc
     8461 agattttccg gtcggcaaga cgggtgatat tcttttttgc ttcttccggt ttatccatgc
     8521 atcgaacgga gtcagtcacg cagtcttcaa acccgtagag aaaaatgtca cctgacgcat
     8581 caagcagaat cctgcgtgct gtcgccaggc attccatcgc ctgcatgaac agtgaaatac
     8641 cttcgttacg ctgcgcgatg atgcggtcga tagccacaga cggaataaca tctgtcgtgc
     8701 cgtcagtatt caggtcagca gtgggtaaag tctgattcat cacattgtct cctcatcgtc
     8761 acgactgccg ggtcagccag gtcatttccc ggaggagcga agctccttct gccgtccact
     8821 tcttcggggt ttctccccgc agtggacctt tccgatgggg gcccgtttac cgcctcacgg
     8881 gacagctgtc cgggggcgga cgcaaaattt tttgccgggt tcatggctaa aaattttgtg
     8941 gccggtatat cttcccctgg actgatggaa cgggaggtgg ttacgtctgc cccccggaaa
     9001 gtccacagcg ggaaacaggc cgcagaggtg gcggcagaag aggagactgc cgcgccatca
     9061 ggcgcggttt ccttacatcc cggcggcctt ttgccgggat gccaggagcg caggccacgg
     9121 cgcgactgaa gaaaaacgac ggcacaacaa tggcatgtcg tggtcactgg tgcaccactc
     9181 ccgcacaggt gaaaacagag aaaacaacac gacatataca atcaaaaaaa agtgaaattt
     9241 cttcactttt tcactctttt ttatcgcgct gacttggtga ctatgatgat aaaataaagt
     9301 gaagtttttt cactttttca aaggtgtttt atgaccggct gggaactgcg aatctggcga
     9361 aaaagcatgc tctggtcacg cgaaaaagcg gccagagaat ttggcgtgac acagagaaca
     9421 tggcacgcct gggaaaatgc ggaacaggtg gatgtcaccg tctggaggac cacccaggca
     9481 ctttcagtcc gtgacctgct gcctcacatg caggggatgc gtaaggcaga tatcatcagg
     9541 cgactggaaa atgaactggg ggaaacggca gaggatgtgt gagcgtgatt cacagcctgg
     9601 ctgaatgcca ggctactgct catcaggaaa cagctttttc agttcttcac cggaaagtcc
     9661 ggttgcctcc tgaatccgtt ccggcggcca gcctatatcc cgaagccggc aggctatttc
     9721 ccgcgctact tcaagagccc cttgtttaaa accttgttta aaaccctcat cgaaaccttt
     9781 ctgtataagg cgttctgcaa tcgtcatgct gtttgctctg ctcctgctga caaaaaaccc
     9841 ggcattttgc cgggctttgt ggttctcatc agtgagtgat gttcttcagt tcagcttcgg
     9901 aaagtccggt gatctgacag atttgtgcag atggcattcc cgttttctga agctgacgcg
     9961 caatatcctg cttctcctcc atgcggcctt tctgtattcc ctgctgtatt ccctgctgta
    10021 ttccctgctg tattccctgc tgtattccct gctgtattcc tttctgtatt ccttgctgct
    10081 caagacgttc ggcaatagtc atcagtttat cctcatgctg cggcagacgc tcagccagcg
    10141 cccgcataaa ttccggtgat acagagtccc ctgcgttaac gatgtaatga atcagaacac
    10201 gcacctgttc atctgaaatt tccaccatga cctgcgggag tttgtccagc agctccatca
    10261 tgtcacgatg gcggatatgc ttcatcagca gcgtcagtgc ggccatccgg cgatggttca
    10321 tgatttcatt gtcatcaacg acagtgatat caaccagcgg aaacggcttt gtgtatattt
    10381 tagccgcaag cgccggatcc tcaaaacagt ccagccagtt catgctgtac gggtacgggc
    10441 tgcgctcacc atgatagaac agcaccggaa tcaccagtgg caatgttttg tactgctcca
    10501 gatgtctctg catcgccgcc acactgtaac gtaacatgcg gaaaggcatg tacagatttt
    10561 cggtgctctg gtgctcgatg agcgtataaa catagccgtc tttacccccg gtggttttca
    10621 cactccagag gatatcgctg gcgtactgat gcaggtccgg ctcaacaaag gaggcgggtt
    10681 ccagcttcag cgtggacagg tcgcacaact gccggtactc cgccggaaga tgcagttcca
    10741 gaaaatctct ggcgacgtcg gggttcgcca ggaacgaccg gaacgcggca tcatgcggcg
    10801 tgggcgttgt ggtgtttttc ttcttactca cggttcctcc atcaaatcag cagcatcgca
    10861 gcaatcatcg tccaaatccc cacaaccaag aggacagcaa ttgcgatcaa agccgtgaca
    10921 acaatagcca cgttctgcgt cacggcgtat ttcttctgca atctcttcag cggaaaggta
    10981 atcaaactcc tcacgggcca gttccaggac cagcagatgt tgttctgtcg gcaatgtagc
    11041 cacaaaatta tcaatagcag cactcaaacg tcgctgatat agctcataaa caagaccaca
    11101 acggtgcatt agttcttcgg ataatagttc cagttttgag cgaacgctgt cagcattgaa
    11161 agtgacttca tcagatgaca tctgaaaatc tttgtcagtg tcaaaacaag acttcatttt
    11221 tatctcctgc ccgtccgggc cctgaatgaa agagaacctg tttcccttct ctgtttttta
    11281 tggaacagga ggaatccggt accatgcgac atagacgtca tgatgttttt catcttgctc
    11341 acggttcctc ctggtaaagt acctgaacat atcctgccac ctgcacagac agacggcagg
    11401 gggtaatcag tggcagaccc gttcaatttc aagcgcacgg agcaggtgcc cccactcgct
    11461 gactttcatg gcggggatac gcacccgctt ccgctcactg cagcgtctgg cgatatgacc
    11521 gcccatcgcc aggacgtccg ggttaatctc atccggtgcc agttcctggc gtctgaagcg
    11581 ggtatggcgg tgcaggggtt tgttctcccg caggcactct tcacgcagac gctttacata
    11641 ctcggcggtt gcgaagataa ttgatggtct catggcataa tctccatagt tagtgaatga
    11701 gatgccggac ttccgtacgg ggaagcgccg gcggagaaag gcagggttta tcgccctgct
    11761 ttttttcatt ccggacagcg acgcagtgcc agcgcgagcg ctttcatctt ccagccttcc
    11821 ggcgtgcggt acatcacctt ccagtagccg tcgatgaaca cataatggaa gcgcacctct
    11881 tctgtgcttt ccgtggcaat agcctccatg aaagaacggt actcacgcgc ctcactgtcc
    11941 atccggaaac cactgtcacg tccgtaataa gccgtcacac cgtccaccgg gttctgaagc
    12001 gtatgcccgg cgggtttgtc gcaacgggga ccgaggcaac ggatatcacc gtgttcaacc
    12061 agggcttcgg cctgctgacc ggtggcatag tgggtatgca ggatgcgacc gttccaggtc
    12121 ggctcaccgt gcaggtggca gtaaacggta cggaacaccc cgtcgcggca aaaaacggaa
    12181 atcgtgctgc tgactgtcat gaaaacgtct ccttctgctg aatcaggtcg ccgggccgac
    12241 cggttgctcc cccggagggg cgaaagcccc ttctgccgtc cacttcttcg gggtttctcc
    12301 ccgcagtgga cctttccgat ggggcagcga cagccacggc acggggcagt tgtccggggg
    12361 cagacgcaaa agtttttgac gcttgcggca aaaagttttg tggctggtac atctccccct
    12421 ggacggctga cacgtgttgt ggccgcttca gcccccggaa agtccacagc gggaaacagg
    12481 ccgcaggggt ggcggcggaa gggcagacaa ctgcgccatc aggcgcagtt tccttacaca
    12541 ccgtcaggtg tgcagggagc gcagatcacg gcgcgacaga ggcggcgcga ccggaacggt
    12601 cgctgcaagc caggtttttc agacatcgga ccgtagcggc agcccgaaac cgcgccagcg
    12661 ggttcggcgg agactggcgg gtgcgcgagc agctgcctgt cggagtcggg cctgacgacc
    12721 ggatgccgcc aggcagccgg gagccgaagg tgtccccgcc tccggaaatc agaacaacag
    12781 aaacgacata acagaaaaat gcctcactcc atgacgggca ggaagggcag cgccgcaggc
    12841 gcggccctcc tgcatttcag gcaggcgtaa gcctgccagg gttttccggg ggcctggcag
    12901 gccatacccc cgccggagcg gggagtaaat caccgacgga acacatgacc gtcaataaag
    12961 gtgaaggagt ccaggaataa atcccgcgca tacgcctcat agtcaaaata gagcgccacc
    13021 gtctccggca cgtcagccag caggccggta tcactgacga actcaaccgc aaaagcctcc
    13081 tcactgtcag cctcgcccca ccaggcatcg cggaagctgt caaaatccgt ctcaccggta
    13141 tcatccaccc agagacgata agcctcttcg cagccttcat cccgtgcctg gcggaagcct
    13201 tcaacccagg cccagttgat atgacattca gaggccatat tccccgggaa tccctcataa
    13261 tcctgaaaca tcagttcagg atcggcttca tcctggtgaa gagcacggca ggcggcgaaa
    13321 aagtcgcgct catcatcaaa cgtggtcagg tcaaaccagc gtccggcgat gcttccacag
    13381 ttgtatttgt gccaggttcc aacgtataca gcaggtgcaa caacagacat tcccgaatcc
    13441 tccatagtca catgttctgc ggcaccgggc ggtgccgtgc ctccataaaa gccgggcgat
    13501 aaggcgggga tgcacagggc gcaagggaag ccgcagcctg acgcacacgg ctggcgggag
    13561 ttggagggtg agaccgcgaa atctgagcgg gtgaacgacc ggaaaatcac ggcagacggg
    13621 aaggagggac cgggtgaccg ccgcgtgcat tttacccttg catccccgcc agccggctaa
    13681 gatggaggca cggcacagcc cgcacacgac gctgaggcca cataactgaa aacccacaag
    13741 gccatgcccc gcatggccgg gtaaaagcgg agccgccggc gacttcctta ttccggcgag
    13801 tgcagaggac acgccgcatg acctccagac cacccctgat atgaaacgat catgatcggc
    13861 gtgtccctgc gaaaaatata catgcctggt tcaccggaga atatctgtga tatccacaag
    13921 ttttctgtcg cacagccagt cctgcgccgc acgcactacg ctactgtccg gtttcaccgc
    13981 tttcagagaa gatgccagtc gtttgatcag catcaccagt tcccgtgtct gtatgtccgg
    14041 cattgtctcc ggcggagcag agggactctc atttccgacg cccatcccat gcgcttccag
    14101 tgtggcacgg acaaaccagg cagcatcgac cagttgctgg cgaatccagg ccagatcggc
    14161 atttctgtca cgggctgcca ttcgctcacc cgacagcgcc ttgctgatat ggctgcgcac
    14221 cagattatcc aggagttcct gcgcttcttc cggtgaaaag ccacctggta tctctgtgcg
    14281 caacagtcct ttcctgtaca gtagctgcag cagatcaatc tgcgaccgca ctgcaactat
    14341 cagtacataa cggacaatat tcgcactggc ggtggtttct gtatcattca gcagttcctc
    14401 cggatcatta ccaccggtat tgcagagcaa cggcaacaga ggtcgcatcc acacgggaac
    14461 ctgatatttt tcgtctgatt tcatcatatt gtgttctcca gtttgttcgg gatcatccac
    14521 tgctcaagtg cacgtacctg taccggtgca ccacgaccgg cctcaccggc ttcaaaaatc
    14581 agccgccaga gtggttcctc ctgccagaaa aaccggtcaa atgatgacag tgacggaaaa
    14641 cgcccgcacc acccccagaa aatcgtttcc agatcatgct cctcaacaac accagaccag
    14701 cgggacaacc agcttccgac cgtggcggga gtatgaaacg caaggtcaat ctgcgcctga
    14761 gcaaaacatg tctggtattc cagttcccgt cgttctgcct cacgttcccg ctggcggctg
    14821 tatttctgct ctgtcagcgt ggttttctga cgacgccgct caccttcacg aaaaggaacg
    14881 gacgggaaca gacggtgccc cgcatcggat ggcaggggaa gaacacagag ttcaccaaaa
    14941 ctttcaatca gtttatccac ggcagccagc cacaggagac ggttgccatg aaattcctcc
    15001 ttcgtcagcg caggagcaaa aaagcggaga tcggtcagcg aaatccggcg actgccattc
    15061 aggcagcgaa agaaagcgct ggcataaggc catgctgaaa gtgtgcgtcc ctgacgacga
    15121 cgttgttcgg tgacagccac agcctgcatg gcggcctgtt gttctgcgct gagggggatc
    15181 agtgcccgtg agcgtgcgct catgcctgac ctccttctgc catcaggata tggatgatgt
    15241 cgttaacact gtacccctgc gcatcaagtg tcgcggcaag cgccagcgtg tgactgatag
    15301 cgacaggcgt gaagatatgt tgcacctgaa cgacggcgaa aggctgtcca ccacaattca
    15361 ccagaaccag catccagagc tgagagacgt ctcccggcga acataatgcc aggtggaaac
    15421 gttcgtgggc tggcgtgaaa cgaacctgga cggggaaaaa gcccccgaac tcactgccat
    15481 actcgccatt catcatccag ttagccggtg cgtccacttc acgcatcacc tcgtgagtga
    15541 gattacggcg catatcgctt ccggcagccc ggatatcttc atattcctgt gcgttcatgg
    15601 actgcagggc attcagggtc agttctgttt tcatcacggt atctcccttg ctgccgcccc
    15661 gtctccggcg gggcggtgtg gttgttcatc aggcggcatc agccaggtta gtggtgtgtt
    15721 cagtggcatc catctgtgga cgtggtgtac acatccagcc aggcacccag cggttgtctt
    15781 tcatatggaa ctctgcatgt tcagccgcat cgcctttctt cattttctcc gcgtcccgcg
    15841 cagcaccgga cagtccggca tcattcaggg ctgcgataat ctgcggcttt ttcaggtgtc
    15901 cgaagaagtt tgcttttgtc ggctgccacc agtcgcgcat gtgaaaaccg atggcgctct
    15961 ccagcgtgtc cagcgggctg cgtgacgtgt gaccacactc gcgggtctga acgccgtgga
    16021 tgctgcacgc ggtgcagaaa ctgagcaggg ataacagcac ctcctgactg agtgaaagga
    16081 acgtcgtcat gtcccgtgac catccctccg gtaacagggc cgcaagacgg gctttttctg
    16141 ccatcatcgc catgaacgcg gcaccttcct tccctgacgg cgcatcgctg gtcagcgaat
    16201 aatgtttaca ttccaggctg atttgtgctg gtttactgta cgctccgctg ccaaacacat
    16261 tcaggcagag cgtccatgcc agcagtgcca gagatttgtc cggttgctgc atcagtgccg
    16321 cctggactgc cagcgtgcgt tcggaagaca ttttcgtcag cagtggcaga ctgatttcct
    16381 ccactgacgc tttctcctgc acctgttccg tgcggtcagc gtcggcagcc gcgtcatcct
    16441 cgctgcgcaa ctgcacacca cgctggacac acacgttgcc ataacgccag gacaccacca
    16501 caccgcttcc ggcacgcatc tccggcgtcc acgccctgac cttcgccatg cagcgcatca
    16561 gcttcatttc tgcttccagc aggtcagatt cctcacactg gttttccagc gcgtcgtagc
    16621 gcgtcatcag ttcgttcagg cgttcttctt cagcttccgt cagcaccgct tccggctccg
    16681 gcaggctgcg gtatgttccg gcatcctcac ggcaggaacc gacaggctcc atacgtccgg
    16741 cgcaccattc ccagccttcg gcttcccgaa ggtgttcagc gacagcctgg agtttttcca
    16801 gcagggcggc atcgagcgcc acgcagtcca catagccacc ctcgtcgtcg ctgaacaggt
    16861 cagtgcgcag ttcatctggc gagaaggcat cagcccccac gaagcggaat ttactgttcc
    16921 ccgccaccgc cacttcactt tcggtcacca gacgacgaat ggtctgcact tccggtttac
    16981 cgccccatcc tgactggcag gcggcttcaa acacctgcac ctgacgcgcg gtgtcgttct
    17041 ccagcgccag cgcctgacag tgctctgtgg tgatgcggtc ttctgccagc gcatcaagga
    17101 tgacaggtgc aaggtctgcc agtttcagca ttcgctgaac gtggcggggt gaatagccca
    17161 gcaaatcacc gatttgtgca ggtgttttgc cttcctgcgc cattgcgcgg aatccggcaa
    17221 tctgttcggc agggtgcata tcccgacgat gaccgttttc agtcattgat gcggcagtcg
    17281 ccagttcctg cggaataatt ttcacgcgga caggccagtc agccggaatg atgccacgct
    17341 ctgccagcat gttgagtgct gccagtcggc gaccacctgc ggcgacaccg tggcggtcac
    17401 ctggcagggc atgaacaacc agattctgca gcaggccgac gcccttaatg gactcagcca
    17461 gttcgctgac ggactccgca gaatacggca ccgtgcgcac attcagcggt gatttaatca
    17521 gtgaagccag cggaacgctc acatcctccg tctgcgccag cagggcagac aggacagttt
    17581 cctgcgtttt tgcaggttta cggctggatt tgcgctctgt ttttgcctta gactctgtaa
    17641 ctgacatata tttctccttt gaccggggtg gttgtatgtc tgccggacgc ctacccgtcc
    17701 ggcacctctc ttacttaaga atgccgaaat ccctgatgac atggttcatc cagtacccgg
    17761 caccgtcctc aaaattccag gtacgccaga ccattgcacc gttattacgg acaaccaggc
    17821 gaaaatgttc tccgttgtca tcctcgataa agacgttccg gtactgtgcg gcaacggctt
    17881 cggcctgttt gcgggtaaaa ggaccgtcag gcagtccctg aagtattctg aaatattcgc
    17941 tcatcctcat atctccggtg atgccccgcg cagggcgggg cggttatcac agtcaggcca
    18001 gtcagaacgg gatatcgtct gaaaacccgt aatcctcccc ctccggcggt tgcggctgag
    18061 gctcgggctg cgcggcctta cgtccacggc ctttcgtttt cgcgccacct tttttcgtcg
    18121 cgtcagcact ctgtggctga ccattctgct gaggctgcgg ttgtgcctga gcgttctgca
    18181 gtggtgcact ccccagcatc tgcatggtgc ccgtggtctt aacaagaatt tcagtgacgt
    18241 aacgggtgat accgttatct tcccagctac gggttcggag ttgcccctcg atatagacct
    18301 gcgcgccctt gcgcagatat tcaccagcca cttccgcgag cttgccgaac agcaccacgc
    18361 ggtgccattc cgtctgctcc cgcatctccc ccgtcttttt gtcgcgccag ctttctgacg
    18421 tggccacctg caggtttgcc actgcgcccc cgttggggat gtaacggacc tccggatcat
    18481 tgcccagacg cccgacgagg atgaccttgt tgataccacg tgcactcatg agaacctcct
    18541 gactgaaaga tgtgagataa accggatacc ggagacgggg cgttcatgtg ccccttcaga
    18601 ggagcgaaag ctcctcctgc cgtccacttc ttcggggttt ctccccgcag tggacctttc
    18661 cgatgggggc ccgtttaccg cctcacggga cagctgtccg ggggcggacg caaaattttt
    18721 tgccgggttc atggctaaaa attttgtggc cggtatatct tcccctggac tgatggaacg
    18781 ggaggtggtt acgtctgccc cccggaaagt ccacagcggg aaacaggccg cagaggtggc
    18841 ggcagaagag gagactgccg cgccatcagg cgcggtttcc ttacatcccg gcggcctttt
    18901 gccgggatgc caggagcgca ggccacggcg cgacaaaggc ggcgcgaccg ttccggtcgc
    18961 tgcaagcccg ggttttcacg catcggaccg tagcgacagc ccgaaaccgc gccagcgggt
    19021 tcggcggaga ctggcgggtg cgccagcagc tgcctgtcgt agtcgggccg acgaccggat
    19081 gccgccaggc agccgggagc cgaaggggcg catgtgtaag aaggttcttt aacgacaggt
    19141 ctgtaacagg tacttcctga aacaacgcct gtcaccaaca ggaagggtaa cgggcgacgc
    19201 gccagcgtgg cccgcttccc ttccggcagg cgccagcctg ccaggggttt tcgcgcgggc
    19261 tgacaggcgg cctccaccgc ctgccctgta tcacctgttt ctgctgccct gaaggatata
    19321 gtgaccaccg aggaaaaccg cctgctgatt cgccacaaaa tcacagattt catcaacaat
    19381 ctgcgcggtg gtgagcggtt tctgccccat ttcacgacgc cgcgcattaa tcacagcttt
    19441 gtaattacgc agaatttcgc gggtttccgt gctgatattc attacccgtt ctccccgtcc
    19501 agcgcctgac gcattacccg cataatctca cgttcttccc tgacaaatgc gtcaccgtcg
    19561 cccccgttct cgctccctac agcatggagg cgataaattt cactaccagc catcaccatc
    19621 actttttcca gcagcaccgc agggactgtt acaagacggt ttacgcgctt ttcctccatc
    19681 agttcattga caatatcgga aacaactctg tcacatgcgc catatacaaa agcatcgtcc
    19741 agccgctgca tgacggtttc cagctcatcg tctgacaggt tccagtcccg ggctatgtgg
    19801 cgcacatccc cgcgggtcag caccacggca aaaacacatt cactgtccgg cacgtctgta
    19861 ttcagcactt cgcggataat ttctttaaca gtacagtaca tgttctcgtc tcctttacag
    19921 gggcggcatc gccgccccgt ccgttaatca gtccagtgct gcgtaaatcg cgttacattc
    19981 gggatgaaat tcaatgtggc tccacaactg cgcatcacgc atccggtaaa ggtgtgtcag
    20041 tcccgcatcc ccgctgtcgt ggtacagcca caactggcgg ttaatcacaa gggaagtcag
    20101 gatgatacct gcggcatcag cactcacagt gcggtcaaac cagttttcac cgttgaccag
    20161 atgaaaacgg tcgccatccg gcatcatgta accgccacca tccggcaggc ggatgaaatg
    20221 ccagaagcct ccctgatatt ccggcatcat tttttgcgcc agcgcgtaca catacgcctc
    20281 ggcatacaga aaatcgttgc caaacaggta aggcagaaag gaaaggcggt cgcactcttc
    20341 aacgttcaga gtgacaggtt tcgcatactg catgggattt cctccgttgt tcacatattc
    20401 cgcgacaccg ggcggtgccg tgcctccaga aaagccgggc gataaggcgg ggatgcaagg
    20461 gcgcaaggga agccgcagcc tgacgcacac ggctgacggg agttggaggg tgagaccgcg
    20521 aaatctgagc gggtgaacga ccggaaaatc acggcagacg ggaaggaggg accgggtgac
    20581 cgccgcgtgc attttaccct tgcatccccg ccagcccggc taagatggag gcacggcaca
    20641 gcccgcacac gacgctgagg ccacataact gaaaacccac aaggccatgc cccgcatggc
    20701 cgggtaaagc ggagcctgcg gcgacatcct tttgctctgt ccggggaccg gcaggaaaag
    20761 gcggagattt ttccccgcca gcgtcatcat ttccggattt tcgccagtgc atctgccatg
    20821 ctgcggattt catcccgtgc ctccagaaca acctcccgca gaatatccct gtcaaaccag
    20881 cgacgggacg gttcatccgg tatacgcagc acatcacaga ccagaccact ggccaggata
    20941 tgctccacac actcacagac caggtacatt tccacagagg aaaaacgggc gccccggttc
    21001 agaatggcat cgagcaccac gggcagattt tcgtcatctt catcaagcca gcaggggtaa
    21061 tcgctttcct tcacaaccag acgaaagcgg cgcgtctggc gggtcagcaa cgtctgaccg
    21121 gtggtggtca gcgccgcccg gatacgccgc gcatcgtctg ccacctggta acagatgctt
    21181 cgtactgcat tgcgcaccgc atcaagaact gcccggattt cttcctccgt cagccattca
    21241 ccggcatacg ggaaagacac ctgcgttccg tctgccggaa gtaccggcag cgcctgccac
    21301 tggctgccgg acagtacaag ttccgccacg gttccggtca gtttttgcat ggcagacgcc
    21361 aggaccggcg gcagcacgcc atcatggcgg ctgataagta acgtcgccag ccgggataca
    21421 tccagtcgca cataaatgct gatccagccc ggatagtgcg gattcagttg ttcaatatcc
    21481 gtctcttccg gccagccctg cgccagcagc aggttacggg cgtggcgaca aaccagtgca
    21541 ttcagcgttt tgttcatgat taatggtctc cctgaatcgc cgcccgaaag cggcgataac
    21601 atcagtcgtt ttcgtgtgtg ttgtttttca gcagggcgcg ggcctgatta accaccgcct
    21661 gacggcgggt gacgcattcc gggacgacga ggccgttatc acgggcggcc cattcacgct
    21721 tccacaatgc ctgttcagcg gtctgaatca gggacgccag cagttcagca gggacggtca
    21781 cctggcggtt ttcactcacg ttgctgataa tctccatagc cgcacccgaa gaaataccgg
    21841 actcagtccg ctggtcttcc gggatgtcct ccaggcgcgc caggacggtc ctgatttcat
    21901 gatcggacag ggaaatatcc attccgtcag caagggcctg aatctcttcc ggagaccaga
    21961 taaccagcat cagcggcgtg tctcccggat actttgctgc cagctcccgg cagagtattt
    22021 cgcatgttcc gtacatactc tctcccctca atctttgaag caggaatcca gttccagaat
    22081 ttcggtatat gcctccatca ggaggcggtt tgcgcgaaac aacgtctgac gcaccagacg
    22141 ttccggcagg gtgtcagcat taaagctgta ccgccgctcc atctcattca tggttgtacg
    22201 caggtcgcac atggcattgg ccacctccgc cacttcggcg cgaagttttt catgtccggc
    22261 atagttcatt tacgcagcct ccggcataaa ccagtcatca tttgcggccc cctgctgcat
    22321 ggcccgctgt acggcagcca gacgttgctg tccggcacgg tgatactgct caagcagctc
    22381 gataccgata tacctgcgtc cggactggag ggcggcgacg caggttgagc cgctgcctgc
    22441 aaacgggtcc agcacaatcg cgttcggatg tgtgaagctc tcaatcagtg gttgcaggct
    22501 ggtaacgggc ttttccgtcg ggtgatggcg gttgcccgaa tatttccagc ccagcacatc
    22561 cggcagcggg ttacggggcc gggccggact gccctttgcc aggatgtaag cgcattcgtg
    22621 gcggtacgcg acatacgcag atttggatgt gtagtttttg gtgaacacaa ggtgccctgc
    22681 cacgctgaat ccggcgcgtt tccaggctgc cataaagcgg tcaatacggt tccagccata
    22741 gaaactcacc atcagcgcat cttttttgag tacgcggtac atttcattgc tggcaggttg
    22801 cagccagtca tcattaacgt caccggcaat ggtgcgcccc tgacggtcac ggaaaccgac
    22861 gagatatgga ggatcggtaa gggtgcgacg tgaaagtcgc acagtttgtt gttttaactt
    22921 catgtaaagg aagtagtaaa accaggcgtt ccgtcgcctg tagcctaccg aagcatgcag
    22981 agcgggtaac tgttcggtat gaagccttcg ggacaacgta gcccggtttt cacaccatgt
    23041 ggaatccgcg aggaggaagc atgtctgcca gtgtcagggc ggatggggcg caaggctgga
    23101 tggcagggtt aacacatgtg aactgctgat aaacatcgtc aaccctgaac gggccaaagg
    23161 cactgacagg cccgacccaa aatgggaacg tagctggacc ttcctctttt gactggggag
    23221 gcacggacat caaagctacc ggtggatagg cagaacctaa cccatccctg caaactgtgc
    23281 ggaacacggt aagcccgtat cactgccctc agggcaggct aaccgcgagg aaagctgtca
    23341 gtgatgcggg tacaggagtg cggagaaagc gaatgccatc ctgtaatggg atggacagga
    23401 gttgagctgt ttcagcaaca tcattccacg cgaaagcggg cagacttccg catggtcttt
    23461 caacacgaga taacctgaat acctttgaag gagggtaagc agatgactgc gaaaaacata
    23521 aacgcgggtg cacccaccga catcgtgact cactggcaca atatcaactg ggccaagtgc
    23581 cgtcgcgagg tcagaaggct gcaggcgcgt atcgtgaagg caatacagga ggagaaagcg
    23641 ggcaaggtaa aagccttgca atggctactc acccactcgt tcagcgccag agcaatggca
    23701 gttaaacgag tgacagataa cagagggaag agaacgccgg gtgttgatgg caaaacatgg
    23761 tcaaaacccg gcagcaagat gaaggctata tacacgctga aaagaagggg ttacaaacct
    23821 cttccgctac ggcgtatata tatcccaaaa tctaacggca aaaagcgacc attagggatc
    23881 ccgacaatga aagacagagc catgcaggca ctctatctga tggcgctgga acctgttgca
    23941 gaaacaacgg ctgacccaaa ttcgtttggg ttcaggccct gtcgctcaac agccgatgcc
    24001 attgagcaat gtttcacaac gttgcacagg gctgatcggg cacaatggat tctggaagcc
    24061 gacattcgca gttgttttga tgagatttca catgaatggc taatcgccaa tatcccaacg
    24121 gatacggcga tcctgaaacg atggctgaaa gccggatata ttgatttggg gaaattatac
    24181 ccaaccagtg caggaactcc acagggcgga ataatatctc caacgctggc aaacatggtt
    24241 ctggatggtc tgcagcctct gttgaagaaa accttctatc gaggcggact gaatccagaa
    24301 aagatcaaca taatccgtta tgcggacgat tttgtgatca cggggataag ccacgacacg
    24361 ctgtcagaaa aggttcttcc cttgttagaa aactttctgg cagaaagagg gcttaccctc
    24421 tcaccagaaa agacacgaat cacacacatc agtgatggat tcgactttct gggtatgaac
    24481 atccgcaaat ataacgggaa actgttgatt aaaccttcac cccaaaaggt aaaggaattc
    24541 ctgaaaaggg tccgccagac gataaaagac aatccgacag tcagccagag cacactgata
    24601 tggcatctca acccagtcct caaaggctgg gtcagatact atcgtcatgt tgtttcaaaa
    24661 aagatctttc agaaagtcag tcatgaaata tggaaagcac tatggcgctg ggcatgtcga
    24721 cgacacccaa agaaaggtac aggctggata agaaagaaat actttcgttc agttaacttc
    24781 cgacactggg tattcggcac agaaacaggg aagctcctgc catcaggcaa acgtgagtta
    24841 ctggcgctat atgaaatctc ggatatgcca gtcaaaagac acagacaaat aaaaagcact
    24901 gctaaccctt ttgatcccag ctacgaatcg tactttgaag cacggctaaa agctacgatg
    24961 ctggaatcca tacagggtta ctctcgccta agaaatctgt ggctccgaca gggaggtcag
    25021 tgccccgtct gccacctgcc agtgaacgcc acaacaggct ggcaacttcg acacattgtg
    25081 ccgaaatccg aagggggact ggataacata ggtaatctgg ccatgttaca tccggcatgt
    25141 catcacactg caaaacatca gggaatatct gttatgaagc cggctctaat gtggagttat
    25201 tgaaaggctt gagccgtatg ccgggaaact ggcacgtacg gttcttaggg ggcggctgca
    25261 cagtaatgtg cagctgctac ccgacatgaa atcaatggcg ttatcaggaa tacgggccat
    25321 aacatcaatg cagttaccaa ggacaaaacg ggacatacaa aactccttct cttacagagg
    25381 gctccgggag ggcacccctg tggtgcccgt tccccgctgc ctgtgggctg ttcaccgcgt
    25441 ccggtaacgg gtcaggccga ccgtggaata ccgcagccgt cgtctttttg acggcaaggt
    25501 tatgccgcga aagcggcttg aagggcgttc atcagaaaag ggcgacgggc tggcgtacac
    25561 tgtcaccgac gccagccggg agcggtaaaa gcgcccgtcc cgtgcccgcc ggacgcggga
    25621 cactgaccac aggcagcgtt tatgccgcgc cgcgtcagcc ggcgcagtaa aaagaggagc
    25681 acggcgacat cctttaccgg aaaagaaacg ggcggttgcc cgcccgtctc cgtcatactt
    25741 catccgccat aatgcgccac cttgtcgact atccacgcag gtccggtgac ttccggcagc
    25801 tcatcgtcat ctgtcgggga agaccattcc agttcccccc acagcacatc aacgagctct
    25861 ccgcgctcat acaactgcca gccacagaaa tcacagccct gttcggcata ccagtgctcc
    25921 agcgtgcagc tgaaacggcg gcttaattct gcaataacgt cgctttccgg ctgacaccac
    25981 ggcgtgtcga aatccacctt aatgaagttg tctccctgac tgctgatatt cacctcatac
    26041 ccacaaggcc acttcacgcc atactgttcc gtataccagt gatatgcaga aggaacaccg
    26101 ttcagaacac caccgttaaa gccattcact tcaacatcca ggcgggtcgg cagaaccgtc
    26161 agcaggtcaa acggcatggt tttttccggc agtacgttgt cacacagacg gttccaccac
    26221 accgatacat cctcgttact ccagaagcca caccagtcac ctctttttgc ggtgaaaagt
    26281 gcggtgatgg tatccctgac atcgtccggt aatccttccc agcgacgctg tccagtaccg
    26341 ctctgcagcc agagttcatg cagcatcagg cagttttgct catccagcag tacaccgtcc
    26401 tgcaggtgtg tcagccagcg ggtgaacgcg atattctccg gcgatacaac accacgtccg
    26461 gcagccgtca gtccggggca cggttcaaac tgcacatctt cagtggtctg cagaagtccg
    26521 gcacttccgg ccagaaacag ctgaatacct tcatttgtgg cgcggcgata aagcggtgtg
    26581 actgcaccgc tggccagtcg tttaatctca gtaatctgtg ccggttcggc agaaaaatac
    26641 atacgattcg agcaccagtt tggcatagga attctcctct cacatgcggc ctccatcagg
    26701 ccgtgccgca cctgtaaccg ggcgaccgca tacgaatgca ggtgcggccc cgaagccgca
    26761 gcgcgtggag gaggaacgac gaaacgtgac cgggtgagga ggccggcgac agcgacacac
    26821 ttgcatcgga tgcagcccgg ttaacgtgcc ggcacggcct gggtaaccag gtattttgtc
    26881 cacagaaccg tgcacaaaat gctgtggata agctggatac agcactccac agcaggcata
    26941 cagccacccc ctcagagaat tgatcgcttt ttagtcagaa caatgacatt tgactgtgtg
    27001 cggattcagt tattagaata ctgtatacat atacagcaaa agaaaaggga gatgagaaca
    27061 tgatccgcat tgaaattctt ttcgaccgcc agagcacaaa aaacctcaaa tcaggcacac
    27121 ttcaggcgct acagaatgaa atcgaacaac gtctgaaacc acactacccg gaaatctggc
    27181 tgcgtataga ccagggcagc gccccgtctc tatctgtcac aggagcccgc aacgacaagg
    27241 ataaagaacg tatcctgtct ctgctggaag aaatctggca ggacgacagc tggctgcctg
    27301 cagcatgagt accgtttatc accgtccggc tgccccttca ggcgatgaca gttacgtgcg
    27361 accgttgttt gccgatcgtt gccaggccgg ttttccttct cctgccactg attatgctga
    27421 gcaggaactg gatctgaaca gctattgcat cagcagacct gcagccacct tctttctgcg
    27481 cgccagcggt gaatcgatga accaggctgg cgtgcagaat ggtgatctgc tggtagtgga
    27541 cagggccgaa aaaccacaac acggggacat cgttatcgct gagatcgacg gggagttcac
    27601 cgtcaaacga ctgctgttgc acccacgccc ggcactggag ccggtttcag acagcccgga
    27661 gttccgcaca ctgtatccgg aaaacatctg tatttttggt gttgtcactc acgtgataca
    27721 caggacgcgg gagttacgct gatgtttgca ctggctgata tcaacagttt ctacgcctca
    27781 tgtgaaaaag ttttccgccc ggaccttcgc aacgaaccgg tcatcgtact cagcaataac
    27841 gatggctgtg tgatcgcgcg cagcccggag gcaaaagccc ttggcatcag aatggggcag
    27901 ccctggtttc aggtgagaca gatgcgcctg gagaagaaaa tacatgtatt ttccagcaat
    27961 tatgcgctgt accacagcat gagccaacgg gtcatggctg ttctggagtc gctttctccc
    28021 gcaattgagc cctactcaat tgatgaaatg tttattgatt tgcgggggat aaatcattgc
    28081 atctctccgg agttttttgg tcatcagctc agggaacagg taaagagctg gacaggactc
    28141 accatggggg tgggcattgc gcctacaaaa acgctggcta aaagtgcaca gtgggcaaca
    28201 aagcaatggc cacagttttc cggagtggtc gcgctgacgg cagaaaaccg taatcggacc
    28261 ttgaagctac tggggctgca gccagttggt gaggtctggg gagtaggacg cagactgacg
    28321 gaaaagctga atgcgctggg tattaacaca gcactgcagc tggcgcaggc taacacggca
    28381 ttcatccgga aaaacttcag cgtcattctt gagcgtacgg tacgcgaact caacggcgag
    28441 tcctgcatat ccctggaaga agcaccaccg gcaaaacagc agattgtctg tagtcgcagt
    28501 tttggtgaac gaatcacaga caaagatgcc atgcaccagg ctgttgttca gtatgcagag
    28561 cgggccgcag agaaactacg tggggagcgt cagtattgcc ggcaggtgac gacatttgta
    28621 cggacatccc cctttgcagt aaaagaaccc tgttacagca atgccgctgt ggaaaagctt
    28681 ccattgccca cacaggacag ccgggacatt attgccgccg catgcagagc cttaaaccat
    28741 gtctggcgtg aagggtaccg ctatatgaag gcaggagtca tgctggctga tttcacacca
    28801 tcgggtatag cgcagccggg attatttgat gaaatccagc cccgtaaaaa cagtgaaaag
    28861 ttaatgaaaa ca
//
LOCUS       DAZFKF010000060        28667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFKF010000060 DAZFKF010000000
VERSION     DAZFKF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.060"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..617)
                     /locus_tag="ACHKA1_003094"
     CDS             complement(<1..617)
                     /locus_tag="ACHKA1_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO5680708.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERA"
     gene            complement(688..2802)
                     /gene="fusA"
                     /locus_tag="ACHKA1_003095"
     CDS             complement(688..2802)
                     /gene="fusA"
                     /locus_tag="ACHKA1_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO5680709.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2899..3369)
                     /gene="rpsG"
                     /locus_tag="ACHKA1_003096"
     CDS             complement(2899..3369)
                     /gene="rpsG"
                     /locus_tag="ACHKA1_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO5680710.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3466..3840)
                     /gene="rpsL"
                     /locus_tag="ACHKA1_003097"
     CDS             complement(3466..3840)
                     /gene="rpsL"
                     /locus_tag="ACHKA1_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO5680711.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3966..4253)
                     /gene="tusB"
                     /locus_tag="ACHKA1_003098"
     CDS             complement(3966..4253)
                     /gene="tusB"
                     /locus_tag="ACHKA1_003098"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO5680712.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4261..4620)
                     /gene="tusC"
                     /locus_tag="ACHKA1_003099"
     CDS             complement(4261..4620)
                     /gene="tusC"
                     /locus_tag="ACHKA1_003099"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO5680713.1"
                     /translation="MKRIAFVFSTVPHGTAVGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4620..5006)
                     /gene="tusD"
                     /locus_tag="ACHKA1_003100"
     CDS             complement(4620..5006)
                     /gene="tusD"
                     /locus_tag="ACHKA1_003100"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO5680714.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANHLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(5006..5728)
                     /gene="yheO"
                     /locus_tag="ACHKA1_003101"
     CDS             complement(5006..5728)
                     /gene="yheO"
                     /locus_tag="ACHKA1_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5680715.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5895..6707)
                     /gene="fkpA"
                     /locus_tag="ACHKA1_003102"
     CDS             complement(5895..6707)
                     /gene="fkpA"
                     /locus_tag="ACHKA1_003102"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5680716.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6928..7146
                     /gene="slyX"
                     /locus_tag="ACHKA1_003103"
     CDS             6928..7146
                     /gene="slyX"
                     /locus_tag="ACHKA1_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO5680717.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7195..7785)
                     /gene="slyD"
                     /locus_tag="ACHKA1_003104"
     CDS             complement(7195..7785)
                     /gene="slyD"
                     /locus_tag="ACHKA1_003104"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO5680718.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7880..8080)
                     /gene="yheV"
                     /locus_tag="ACHKA1_003105"
     CDS             complement(7880..8080)
                     /gene="yheV"
                     /locus_tag="ACHKA1_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO5680719.1"
                     /translation="MAIRKRFIAGAKCPVCQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8090..9895)
                     /gene="kefB"
                     /locus_tag="ACHKA1_003106"
     CDS             complement(8090..9895)
                     /gene="kefB"
                     /locus_tag="ACHKA1_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO5680720.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHTDT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9895..10446)
                     /gene="kefG"
                     /locus_tag="ACHKA1_003107"
     CDS             complement(9895..10446)
                     /gene="kefG"
                     /locus_tag="ACHKA1_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO5680721.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10577..12490
                     /gene="yheS"
                     /locus_tag="ACHKA1_003108"
     CDS             10577..12490
                     /gene="yheS"
                     /locus_tag="ACHKA1_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5680722.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDDAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12490..13512
                     /gene="yheT"
                     /locus_tag="ACHKA1_003109"
     CDS             12490..13512
                     /gene="yheT"
                     /locus_tag="ACHKA1_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO5680723.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13506..13724
                     /gene="yheU"
                     /locus_tag="ACHKA1_003110"
     CDS             13506..13724
                     /gene="yheU"
                     /locus_tag="ACHKA1_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO5680724.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13778..14647
                     /gene="prkB"
                     /locus_tag="ACHKA1_003111"
     CDS             13778..14647
                     /gene="prkB"
                     /locus_tag="ACHKA1_003111"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO5680725.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14702..15106)
                     /gene="yhfA"
                     /locus_tag="ACHKA1_003112"
     CDS             complement(14702..15106)
                     /gene="yhfA"
                     /locus_tag="ACHKA1_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO5680726.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15408..16040
                     /gene="crp"
                     /locus_tag="ACHKA1_003113"
     CDS             15408..16040
                     /gene="crp"
                     /locus_tag="ACHKA1_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO5680727.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16091..18181
                     /gene="yhfK"
                     /locus_tag="ACHKA1_003114"
     CDS             16091..18181
                     /gene="yhfK"
                     /locus_tag="ACHKA1_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO5680728.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTAPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRIIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKT"
     gene            complement(18248..19468)
                     /gene="argD"
                     /locus_tag="ACHKA1_003115"
                     /gene_synonym="dapC"
     CDS             complement(18248..19468)
                     /gene="argD"
                     /locus_tag="ACHKA1_003115"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO5680729.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     YQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19554..20117)
                     /gene="pabA"
                     /locus_tag="ACHKA1_003116"
     CDS             complement(19554..20117)
                     /gene="pabA"
                     /locus_tag="ACHKA1_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO5680730.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20149..20751)
                     /gene="fic"
                     /locus_tag="ACHKA1_003117"
     CDS             complement(20149..20751)
                     /gene="fic"
                     /locus_tag="ACHKA1_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO5680731.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20741..20908)
                     /gene="yhfG"
                     /locus_tag="ACHKA1_003118"
     CDS             complement(20741..20908)
                     /gene="yhfG"
                     /locus_tag="ACHKA1_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO5680732.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(21013..21585)
                     /gene="ppiA"
                     /locus_tag="ACHKA1_003119"
                     /gene_synonym="rotA"
     CDS             complement(21013..21585)
                     /gene="ppiA"
                     /locus_tag="ACHKA1_003119"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO5680733.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21856..23037
                     /gene="tsgA"
                     /locus_tag="ACHKA1_003120"
     CDS             21856..23037
                     /gene="tsgA"
                     /locus_tag="ACHKA1_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO5680734.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23299..25842
                     /gene="nirB"
                     /locus_tag="ACHKA1_003121"
     CDS             23299..25842
                     /gene="nirB"
                     /locus_tag="ACHKA1_003121"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO5680735.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLIGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25839..26165
                     /gene="nirD"
                     /locus_tag="ACHKA1_003122"
     CDS             25839..26165
                     /gene="nirD"
                     /locus_tag="ACHKA1_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO5680736.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26291..27097
                     /gene="nirC"
                     /locus_tag="ACHKA1_003123"
     CDS             26291..27097
                     /gene="nirC"
                     /locus_tag="ACHKA1_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO5680737.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27116..28489
                     /gene="cysG"
                     /locus_tag="ACHKA1_003124"
     CDS             27116..28489
                     /gene="cysG"
                     /locus_tag="ACHKA1_003124"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO5680738.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6942 a         7704 c         7229 g         6792 t
ORIGIN      
        1 gcacgctctg gttccggaat gtaagaatcc aggaagccag ccagttccag gattttcgct
       61 tcccactctg cgtcgccttc cagcgctttc agagcagaac cacgaacgat cggagtgtcg
      121 tcgcccggga agtcgtactg agacagaagt tcacgaactt ccatttcaac cagttccagc
      181 agctcttcgt catcaaccat gtcgcatttg ttcaggaaca cgatgatgta cggaacgcct
      241 acctgacgac ccagcaggat gtgctcacga gtctgcggca tcgggccgtc agtcgcagca
      301 actaccagga tcgcgccgtc catctgagca gcaccggtga tcatgttttt aacatagtcg
      361 gcgtgccccg ggcagtctac gtgtgcgtag tgacgggtcg gggtgtcata ttcaacgtga
      421 gaagtgttga tggtgatacc acgagctttt tcttccggcg cgttatcgat ctggtcgaat
      481 gcacgagcag caccaccgta ggttttagcc agtacggtgg tgattgcagc ggtcagagta
      541 gttttaccgt ggtcaacgtg gccgatagta ccaacgttaa cgtgcggttt tgtacgttca
      601 aatttttctt tagacacggc tatattcctt actatagtgc tctccccttc aggagagagc
      661 acgggacttt ggtattaacc cttaggctta tttaccacgg gcttcaatta cggcctgagc
      721 aacgttactc ggcgcttcat catacttcag gaattccata gtgtatgatg cacgaccttt
      781 ggtcagagaa cgcagctgag ttgcgtatcc gaacatttca gacagcggta cttcagcgtg
      841 gatcttaacg ccagtaactt cagattcctg acctttgagc ataccacgac gacggctcaa
      901 gtcaccgata acgtcaccgg tgttctcttc cggagtttct acttcaacct tcatgatcgg
      961 ctcaagcaga actggtttcg ctttcttaaa gccttcttta aaggcgatag aagcagccag
     1021 tttaaacgcc agttcagagg agtcaacgtc atggtaagaa ccgaagtgca gacgaatacc
     1081 catgtctact accgggtagc ctgccagcgg acctgctttc agctgttcct ggataccttt
     1141 atcaacggcc gggatgtatt cgccagggat tacaccacct ttaatgtcgt tgatgaactc
     1201 gtagcctttc gggtttgaac ccggctccag cgggtacatg tcgataacaa catgaccata
     1261 ctgaccacga ccaccagact gtttcgcgtg tttaccttca acatcggtaa ctttctggcg
     1321 gatagtttca cggtaagcaa cctgcggttt acctacgttc gcttcaacgt tgaattcacg
     1381 cttcatacgg tcaacgatga tgtcgaggtg cagttcgccc atacccgcaa tgatggtctg
     1441 gttagattct tcgtcagtcc atacacggaa agacgggtct tctttagcca gacggcccag
     1501 agccagaccc attttttcct ggtcagcttt ggttttcggt tcaactgcga tggagattac
     1561 cggctcaggg aattccatac gttccagaat gatcggcgca tccgggtcac acagggtgtc
     1621 accagtggtt acgtctttca gaccgatagc agcagcgatg tcgcccgcgc gaacttcttt
     1681 gatctcttca cgtttgttag cgtgcatctg aacgatacga ccgaaacgct cacgtgcagc
     1741 tttcacggag ttcagtacgg tatcaccaga gttaaccaca ccggagtaaa cacggaagaa
     1801 ggtcaggtta ccaacaaacg ggtcggtagc gattttgaac gccagtgcag agaacggctc
     1861 gtcatcactt gcgtgacgtt cagccggagt gtctttaccg tcgtccagga taccgttgat
     1921 cgcaggtacg tcaaccgggg atggcaggta atcaattacc gcatccagca tcgcctgaac
     1981 acctttgttc ttgaacgcag aaccacaggt taccaggatg atttcgttgt tcagaacgcg
     2041 ctgacgcaga gcacctttga tttctgcttc agtcagttct tcaccaccca ggtatttttc
     2101 catcagctct tcagaagctt cagctgcgga ttcgatcagg ttctggtgcc attcgttagc
     2161 cagttcaacc atgtctgccg ggatatcttc gtattcgaag gttacgccct ggtcagcgtc
     2221 gttccagttg atagctttca ttttcaccag gtcaacaaca cctgtgaaat gttcttcagc
     2281 accaatcgcc agctgcagcg gaaccgggtt cgcgcccaga cgggttttga tctggttaac
     2341 aactttcagg aaattcgcac ccatgcggtc cattttgtta acgaacgcaa tgcgcggaac
     2401 tttatatttg tttgcctgac gccatacggt ttcagactgc ggctgaacac caccaactgc
     2461 gcagtaaacc attaccgcac catcgagaac acgcatggaa cgttctactt cgattgtgaa
     2521 gtcaacgtgc cccggggtgt cgatgatgtt gatgcgatgc ggctcatact gcttagccat
     2581 accagaccag aatgcagtag tcgcagcgga agtgatggta ataccacgtt cctgctcctg
     2641 ctccatccag tccatggttg cagcgccgtc atgaacttca ccgattttat ggtttacacc
     2701 ggtgtagaac agaatacgtt cggtagtagt ggttttaccg gcgtcgatgt gcgcactgat
     2761 accgatgtta cggtagcgtg cgatgggtgt tgtacgagcc atttgtttcc tcgtttatct
     2821 tttaggcgtt caatttaagt agcccaaagc gggctgctta ctggaagcgc ccgcctggtg
     2881 actaaaactc cgaagggatt accaacggta gtgtgcgaac gccttgttgg cttcggccat
     2941 acggtgaacg tcttcacgtt tcttaactgc agtacctttg ttttctgcag catcagaaag
     3001 ttcgttcgcc aggcgcagag ccatggattt atcaccgcgt ttacgagcag cttcaacgat
     3061 ccaacgcatt gccagagcat tacgacgaac cggacggact tcaactggta cctgataagt
     3121 agaaccacca acgcggcgag acttaacttc tacagtcggg cgcacgtttt cgagagctac
     3181 ttcgaatgct tccagttcag atttaccaga gcgctgagcc agggtctcca gcgcgctgta
     3241 tacgatagat tcagcagtag attttttacc atctaccatc aggatattta caaatttagc
     3301 cagcagttct gatccgaact tcggatccgg cagaatttta cgctgaccaa tgacgcgacg
     3361 acgtggcatg gaaatactcc gttgttaatt caggattgtc caaaactcta cgagtttagt
     3421 ttgacattta agttaaaacg tttggcctta cttaacggag aaccattaag ccttaggacg
     3481 cttcacgcca tacttggaac gagcctgctt acggtcttta acgccggagc agtcaagcgc
     3541 accacgtacg gtgtggtaac gaacacccgg gaggtcttta acacgaccgc cacggatcag
     3601 gatcacggag tgctcctgca ggttgtgacc ttcaccaccg atgtaggaag tcacttcgaa
     3661 accgttagtc agacgaacac ggcatacttt acgcagcgcg gagttcggtt ttttaggagt
     3721 ggtagtatat acacgagtac atacgccacg tttttgcggg catgcttcca gcgcaggcac
     3781 gttgcttttc gcaactttgc gagcacgtgg tttgcgtacc agctggttaa ctgttgccat
     3841 taaatagctc ctggttttag cttttgcttc gtaaacacgt aataaaacgt cctcacacaa
     3901 tatgaggacg ccgaatttta gggcgatgcc gaaaaggtgt caagaaatat acaacgatcc
     3961 cgccatcacc aggccatctg gctggagtgc ttaaccgtaa gtctgacgaa atcagtatag
     4021 tcaatgggaa tgatgtcgtt cgaaatttga ccagtcaaac cgcgggcaat aaggtcttcg
     4081 ttcagggcat agaccttaat gggggcatta cgcagacttt caaggtagcg gttaccgtca
     4141 actgcggcag ttacgccatc ttgcaataat agcagttcgt cgccttcact gagcagacgc
     4201 agcagcgcag caaaatccgt cagccagggt gaacgatgta acgtgtgcag cataaacgcc
     4261 tcaaaacctc aaaataacat cgtagttggc gagttcgcgg cgtaaggcat ctgcttcgag
     4321 cggcgtggct tcgacaacaa agggggtctg cggatctaac ccgcgttcgc gcagtgaagc
     4381 cgcacaaacc cagcactgtt caatgtcgta cagacccaac aatttaaaag tggcaatgta
     4441 atcacgcgcc agcactgcat cgggcttttg tcctggcagt agctgaaaaa cgccatcagc
     4501 aataaagaag acggccagct cgtcagttaa cgcggaagtt gccagtaaag catctaaacc
     4561 ttcccgcccg acagcggtac catgaggcac agtagaaaaa acaaacgcaa ttcgtttcat
     4621 cagaactgta ccaccctgtc acaggtcagc gaggcttccg ccagcgcgcc aagtccgctt
     4681 aaggtaaatc cctgctgaag gtttgacgaa gccagcccca gtcttccggc ctccgtttca
     4741 tcaacaacgc cacggcgtaa tgctgccgct acgcagatat tcagcgccac accatgttgc
     4801 gcattcaact gttgccagcc gcgcacaagg tcaaattcgt cgcttgccgg tgaggtcaag
     4861 tggttagcgt tatagacccc ttcccgatag aaaaagacgc tgcttaactt atggccttct
     4921 gctatcagcg cctgcgcaaa ctgaaaagca ctactcgcct gttgcgtacc gtatgctggc
     4981 ccggtcacca cgatggcaaa acgcattact tatcttgccc ctggaaatca ccgctcttga
     5041 actggcggat gtagagatag acagtgtgtt tggagatgtt caggcggtca gcaacctggt
     5101 tgatcgcatc tttaatatcg aagatccctt tctcgtagag attcagcacg atctgacgat
     5161 ttttggcgtt attagaaaca ttgcgatcgg cattcacttc ttcgatggtg aactccagcg
     5221 tttgggtaac cagatcttca acagaagagg caaagttgac gcttgaaccg acatccgggg
     5281 tttctggcgg cacaaaggtg ctcataatct gcgagaaggg aacatcaaga ttcatattga
     5341 tgcacagcag accaattaca cgctgttcgc ggttacgaat cgcgatagtc agggacttca
     5401 ttaatacgcc gcttttggcg cgagtaaagt agcatttaga aacgctgcta tccgctcccg
     5461 tcatatcgtg cagcatacgt agcgccaggt cagtaattgg cgaaccaatc ttccggcctg
     5521 tatgttcacc gttagcaatg cgaatggctg aacattttag atcctgcaaa gagtgcaaaa
     5581 cgatttcaca gtgggagcca ataagcatcg ctaacccgtc caccaccgct tcgtaggatt
     5641 tcagaatatc aaaatcggtc tggtcgaaag gacgttgatc cagtaaatcc aactcactgg
     5701 tttcgttggt taaaagcgac ctggacatga aaaaaaacac tccttttcag gagcctgtcg
     5761 ttaacttttc agggcaggct cattaatgat gcgggtaact aaattaatac agcggaggtt
     5821 ccgctttcca gcactaatta tatccggcct gtaataaaaa aaccgccgcc tggtcaggcg
     5881 gcggttctta atgcttattt tttagcagaa tctgcggctt tcgcatcagc ttccggcttt
     5941 gcatcagcct tcggcgctgg tttcacatcc agcagctcta cgtcaaacac cagggtagaa
     6001 ttcggtggga tccccggaac acccgctttg ccgtaagcca gttctggtgg aataaccagt
     6061 ttgatcttac cgcctttctt gatgttcttc agaccttctg tccaacccgg gataacaccg
     6121 tccagacgga aagaaagcgg ttcaccacgg gtgtaagagt tgtcgaactc tttaccgtcg
     6181 atcagcgtac ctttgtagtt cactacaaca gtatcgctgt ctttcggtgc ttcgccttta
     6241 ccggcttcta ctacctgata aaccagacca gttgaagagg ttttcacacc tttctctttg
     6301 gcaaatttct cgcggtactc tttacctttt gcttcgttat cagccgcgtc tttttccatc
     6361 ttcgcctgag cagaagactt cacgcgagct tcgaatgctt gcagagtctg ttcgatctct
     6421 tggtcggaga gtttgctctt atcagcaaat gcatcctgaa caccagcgat cagctgatct
     6481 ttatccagtt tgatgcccag tttttcttgt tcttttagag agttttccat gtaacgaccc
     6541 agcgaggcac ccagtgcata agctgatttc tgatcgtcat ttttgaacgc tgctttgctg
     6601 tcagcagttg tagcaggttt tgcagcttca gcagcaaaag tgattggtgc atgcagggca
     6661 acggccattg tggtcgccag cagcgttact ttaaacagtg atttcatcca tatctccagg
     6721 atcggggcat ctcaccccag ggttaactat tatcagaagg gtactataaa gcgttgtcga
     6781 acaaatctac atacagacac gccctattat catctatttt cagactcttt ttgtttaaat
     6841 tagtttcgat gaccgcgaaa tgagtgctgt ctcgggcagc aaagttaagt agaatccgcg
     6901 gcgaccattc gacaaaagag gtgaatcatg caggatttat cattggaagc acgcctggca
     6961 gagctggaga gccgactggc ttttcaggaa atcaccattg aagaactgaa cgtcacggtg
     7021 accgctcatg aaatggagat ggcgaaactg cgcgatcatc tgcgtctgct gaccgagaag
     7081 ttaaaagcca gccagccgtc gaacatcgcg tcgcaggctg aagaaacgcc accgccacat
     7141 tattgaggcg taaaaaaagc ggggattccc cgcttttttg tcactttttc ggtattagtg
     7201 gcaaccgcaa ccgccgttac ctttaccgcc acagcagcct tcgccaccgt gctcatgacc
     7261 gtgatcatgg ccatgaccac cgcagcaacc gtcgtggtcg tgatcgtggt gatgatcgtg
     7321 cgcaccgtga acgtggccat gagccagctc ttcttcagtc gcttcgcgaa tcgccacaac
     7381 ttcaacgttg aatttcaggt tctgaccggc cagcatgtgg ttaccatcaa ccacgacgtg
     7441 atcgtcttca accgcagtga tttcaaccgg taccggaccc tggtcggttt cagccaggaa
     7501 acgcatacct acctgcagtt catcaacgcc cataaatacg tctttaggca cacgttgcac
     7561 caggttttcg tcgtactgac cgtaagcgtc gttcgcgcca acagcgacat caaatttgtc
     7621 gccaacttca tgaccttcca gcgccgtttc caggccagag atcagggaac cgtgaccatg
     7681 caggtagtcc agcggcgcac tcaccggaga ctcatcaacc aacacaccgt cttctgtacg
     7741 tacctgatag gccaggctga ccaccaggtc ttttgctact ttcatgatat ctcctgagca
     7801 tgggaagaat agtggcgcag attgtagcgg aattctgcag ccgtgtactc actagcttaa
     7861 aaaaactcgg cgcatatcgc tagtccggat gaaaaatccc gatcacttgc tcatctttgc
     7921 gaacgtgatc gcgagcttct ttgtctgctt ctcgcatctg atgtccgcac ttaacacatt
     7981 caacaatatc aatattattt tcgcgccaca tcgccattga atcctgcgcc tgacagaccg
     8041 ggcattttgc gcccgcaata aaacgttttc ggattgccat ctttaccctc tactcaaatt
     8101 catcccagcc gtccagctgg cgtcgttctt gttgcatttc acgctggaaa atctcttcca
     8161 gttcgcgtcg ggcttccctg gcgcgagaaa tttgtacggt atcggtatgc attgggatga
     8221 gctctcgcag catccgcata tccaggcggc gaaaatgcag ttgcgcccgc tgtgcctgat
     8281 gcggatgcat accaagcgtg accagcgtct tgcgccccag ctctaacgca ctggagaatg
     8341 tttcacggga aaactgcgtc acccctgcct gtaataactc atgcgcttcc acacgtccgc
     8401 gcgctcgcgc aagaatatgc aaatgcggaa agtgctgctg gcatatttcc accagcttca
     8461 tggtgtcttc cggctcgtta caggtaatga cgatagactc agcggcctct gcacccgcag
     8521 aacgtaaaag atctacctgc gtggcgtcgc cgtaataaac tttgtagcca tatttgcgca
     8581 tcaggttaac ggcgctgata tcccgctcca gcacagtaat gcgcatttta tttgccatca
     8641 acaaacgacc aatcacctgc ccaaaacgtc caaagcccac gacaatgacc tggggtttat
     8701 catcgttgac ccacggtttt tcgtcttctt cttccggtcc gttaaactgg cgggatagcc
     8761 atttatccac cagcttcatc agcagcggcg tggtcatcat ggaaagcgtc accgtcacca
     8821 gcaacagcgc catctggtcg ccctggaata agcgttgtga agaagcggta gaaaagagga
     8881 caaaggcaaa ctcaccgccc tgactcaaca cgccagcaaa ctgcatccgc tctgagctac
     8941 gcacgccata caatcgcgcc agcagataca gcacgagaat tttcaccgcc accagcacaa
     9001 ccacgcttat cactacccac aacagatggg tataaagcac cccgaggttg agtgacatcc
     9061 cgacagagat aaagaacaaa ccgagcagca agcctttaaa gggatcgata gccgtttcca
     9121 gttcatggcg atattcactt tccgccagca gcacgcctgc aataaacgta ccgagcgcca
     9181 tcgacagccc caacgcatcc ataaacaatg cggaacccaa taccagcagc agcgtcgcgg
     9241 cggtgaacac ttcccgcacg ccagaagctg caataaaacg gaataccgga cgcaataaat
     9301 agcgcccgcc aatcagcatg ccgacaaacg ccagcacctt catgccgatc ttcatccagt
     9361 cgaaatgttc gtctgccgaa cccgccaaca acggcactaa cgccagtgcc gggatcaccg
     9421 ccagatcctg aaacagcagt accgaaaacc cgagctgccc ggattcgctg cgattcatcc
     9481 ctttctcacg catcaattgc aacgccattg cggtcgaaga catcgcaagg ccaatgccac
     9541 cgaccaccgc cgcctgccag gcgaaatccg tcagcatcaa taatcccgcc agcaacgccg
     9601 cgcttaacag cacctgtgcc gcgcctacgc caaaaatcga acgccgcagt tgccaaagct
     9661 tggagggatt caactcaagg ccgatgataa acatcaggaa caccacgccg agttccgaaa
     9721 agtggaggat ctcatcgacg tcgctaataa accccagccc ccacgggcca attgcaatcc
     9781 ctgccagcaa atatcccaac acagcgccaa tacccagccg cgatgccaac ggcaccgcag
     9841 ccaccgccgc gaagagaaac agcactcctg caagtaaaaa atcggaacct tccatcagcg
     9901 gcctcctgga gacagcggat ttgccagcca gtcaccgtag gctctggcgt ggctcgccag
     9961 ctcctgtgcg ctttgccgtc tcgcccagta aataatgatg ggacttaacc aatgcatccg
    10021 gcacatgccc gccgccagtt caaaggggcg caggacatcg ctcatcgggt agcgattcaa
    10081 cgcgtcataa cggtaagcac tttccggctc gccggtggta atcacactac gccagtactt
    10141 tcccgccagt tggtttcctc ccggcccgct ggcaaaacca cggcttaata cccggtccag
    10201 ccactctttc agtagcgccg ggcagctata ggtataaaga ggatgctgaa agacaatcac
    10261 ctcgtgctcg cgcagtaatg cctgctcacg ggggatatca ataaaaaaat cgggatagtg
    10321 cgcgtaaagg tcgtgcacgg taacattgct gagctgcgtg gccggtttaa gcagtacccg
    10381 gtttgccacc gagtcctgag attccggatg ggcatacagc agcaaaactt tcgctggctg
    10441 agacatcatc cccctcccgg tatggttttt gtgtatagtc gctgttttgg gctaccattg
    10501 cgcccggtgc ggcagctcgc ccatacatta cattatcata atgataagtt aacatagtct
    10561 gaacatacgg caccttatga ttgttttctc ctcgttacaa attcgtcgcg gcgtgcgcgt
    10621 cctgctggat aatgccaccg ccaccatcaa ccccgggcag aaagtcggcc tggtgggtaa
    10681 aaacggctgt ggtaaatcta ccctgctggc attgctgaaa aatgaaatca gcgccgacgg
    10741 cggcagctac acctttccgg gaagctggca actggcgtgg gtgaatcagg aaacgccggc
    10801 gttaccgcaa gcggcgctgg aatatgtcat tgacggcgac cgtgaatatc gtcaactgga
    10861 agcgcagcta cacgacgcca acgaacgtaa cgacgggcac gccattgcga ccattcatgg
    10921 caagctggat gctattgacg catggagtat tcgctcccgt gccgccagcc tgctgcacgg
    10981 cctcggtttc agcaatgaac aactggagcg cccggtaagt gatttctctg gtggctggcg
    11041 tatgcgtctt aaccttgccc aggcgctgat ttgccgttca gacttgctgc tgctcgacga
    11101 accgactaac cacctcgatc tcgatgccgt tatctggctg gaaaaatggc tgaagagcta
    11161 tcagggcacg ctgatcctga tctctcacga ccgcgacttc ctcgatccga tcgtcgataa
    11221 aattattcat atcgaacaac aaagcatgtt cgagtacacc ggcaactaca gttcgtttga
    11281 agtacagcgc gccacccgtc tggcgcagca acaagcgatg tacgaaagcc agcaggaacg
    11341 cgtagcgcat ctgcaaagtt atatcgaccg tttccgtgcc aaagccacca aagcgaagca
    11401 ggcccagagc cgcattaaga tgctcgagcg tatggagctg attgcccccg cgcacgtcga
    11461 caacccgttc cgctttagct tccgcgcgcc ggaaagcctg ccaaatccgt tactgaagat
    11521 ggaaaaggtc agcgcaggct atggcgatcg cattattctc gactcgatta aactgaatct
    11581 ggtccccggt tcgcgcattg gtctgttagg ccgcaacggc gcgggtaaat cgacattaat
    11641 caaactgtta gccggtgaac ttgcgccagt cagtggtgaa ataggcctgg caaaagggat
    11701 caagctcggc tacttcgccc agcatcaact tgaatacctg cgcgccgacg aatcgcctat
    11761 tcaacatctg gcacgtttag cgccgcagga actggagcaa aaactgcgtg actacctcgg
    11821 cggctttggt ttccagggcg ataaagtaac cgaagaaacg cgccgcttct ccggtgggga
    11881 aaaagcccgc ctggtgctgg cattaattgt ctggcagcgg ccgaatctgc tgctgctcga
    11941 cgaaccgact aaccaccttg acctcgacat gcgtcaggca ctcaccgaag cattaatcga
    12001 gtttgaaggc gcgctggttg tcgtttcgca cgaccgtcat ttgctgcgtt ccaccactga
    12061 cgacctctac ctggttcacg atcgtaaagt cgagccgttc gacggcgatc tggaagatta
    12121 tcaacagtgg ttgagcgacg tacaaaagca ggaaaaccag accgacgacg cgccaaaaga
    12181 gaacgcgaac agcgcccagg cacgtaaaga tcagaagcgt cgggaagcgg agttgcgtgc
    12241 gcaaacccag ccactgcgta aagagattgc ccgtctggaa aaagagatgg agaagctgaa
    12301 cgcgcaactg gcgcaggcag aagagaaact cggcgacagc gaactgtatg accagagccg
    12361 taaagcggag ttgaccgcct gcctgcaaca gcaagccagc gccaaatccg gcctggaaga
    12421 gtgcgaaatg gcatggctgg aagcccagga gcagcttgag cagatgttgc tggaaggcca
    12481 aagcaactga tggcgcagat aacgacgacc gatgccaatg aattcagcag cagtgctgaa
    12541 ttcaccccta tgcgcggctt tagcaattgt catctgcaaa ccatgctgcc gcgtctgttt
    12601 cgtcgcaagg tgaaattcac cccgtactgg cagcggctgg agttgcccga cggcgatttt
    12661 gtcgatctcg cgtggagtga agccccagca caggcgcggc ataaaccgcg tttagtggta
    12721 tttcacgggc tggaaggcag tctcaatagc ccttacgccc acggtctggt tgaggcggcg
    12781 caaaagcgcg gctggctggg cgtggtgatg cattttcgcg gatgcagcgg tgaaccaaac
    12841 cgtatgcacc gcatttacca ttcgggcgaa accgaagacg ccagttggtt tttacgctgg
    12901 ctgcaacgcg aatttggtca tgcgccaacg gctgccgtcg gctattcgct cggcggtaat
    12961 atgctggcct gtttgctggc aaaagaaggc aatgatctcc ctgttgatgc ggcggtgatt
    13021 gtctctgcgc cgtttatgct ggaagcctgt agctatcata tggaaaaggg cttttcccgc
    13081 gtttatcagc gttacttgct gaacctgtta aaagccaatg ccgcgcgcaa gctggcagcc
    13141 taccccggaa cactgccgat taatctcgcg cagttaaaat cggtacgtcg catccgtgaa
    13201 tttgacgatc tcatcaccgc cagaattcac ggctacgctg acgctatcga ctattatcgt
    13261 cagtgtagcg ccatgccgat gctgaaccgg atcgccaaac cgacgctgat tattcacgcc
    13321 aaagacgatc cgtttatgga tcatcaggtg atcccgaaac cggaaagtct ccccccgcag
    13381 gtggagtatc aactgactga acatggcggt catgttggct ttattggcgg tacattactt
    13441 catccgcaaa tgtggctgga gtcacgcatt cctgactggt taacaacgta tctggaggcg
    13501 aaatcatgtt gattccgtgg caagacctct cccccgaaac gctggaaaat ttaattgaaa
    13561 gctttgtgtt acgtgaaggc accgattatg gtgaacatga gcgtacactt gaacagaaag
    13621 tcgccgacgt caaacgccag ctacagtgcg gagaagcggt gctggtatgg tcggagctgc
    13681 acgaaacggt caatattatg ccgcgcagtc agtttcgcga ataacctgca accgtcggcg
    13741 gacgagataa taatgatagc taagtcatgg agttactatg tctgccaaac atccggtcat
    13801 tgcggtaaca ggatccagcg gcgcggggac caccaccacc agcctcgcgt ttcgtaaaat
    13861 attcgcgcag ttaaatctgc atgcagccga ggtggaaggc gacagttttc accgttacac
    13921 ccgcccggaa atggacatgg cgatccgcaa agcgcgcgac gccgggcggc atatcagcta
    13981 cttcggcccc gaagcgaacg acttcggcct gctggaacaa accttcattg aatacggtca
    14041 gagcggcaaa gggaaatctc gcaaatatct gcatacctac gatgaagccg taccgtggaa
    14101 tcaggtaccg ggaacattca ccccctggca acctttacca gaacccactg atgtactgtt
    14161 ttatgaaggt ttacacggcg gcgtagtcac gccacagcat aacgttgcgc agcatgtgga
    14221 cttactggtc ggcgtggtgc ctatcgttaa ccttgagtgg attcaaaaac tgatccgcga
    14281 caccagcgag cgcgggcact cacgagaagc agtgatggac tcagtagtgc gttcaatgga
    14341 agactatatc aactacatca caccgcagtt ttcccgcacc catcttaact tccagcgcgt
    14401 tcccaccgtc gacacttcaa acccgttcgc ggcaaaaggt atcccgtcgc tggatgaaag
    14461 ctttgtggtg atccattttc gtaatctgga agggatcgat ttcccctggc tgctggcgat
    14521 gttgcaaggc tcattcattt cccacatcaa tacgttagtg gtacctggcg gcaaaatggg
    14581 gctggcaatg gaattaatta tgctgccgct ggtgcaacga ttgatggaag gaaagaaaat
    14641 cgagtaactt tgctattatg ccggataaaa tactatccgg cttcacaacg ggatagttaa
    14701 atcacgcggc aaccacttca tacgagtgag taatattcac cgctttttcc agcatcaacg
    14761 ccactgaaca atatttctca gcagagagat caaccgcacg cgcaaccgct gcgtctttca
    14821 ggtcgcgacc ggtgacgata aaatgcagat taatgtgcgt aaacaggcgt ggtgcctctt
    14881 cgcggcgttc agaggtcaat tttacttcac aatcgaccac atcctgacgc cctttttgca
    14941 ggatagatac cacatcgatg gcactgcaac cacccgccgc catcaacacc atttccatcg
    15001 gacttggtgc tttatcgcct gagttgccgt ccattaaaat ctgatgacca gaggcggatt
    15061 cgcccagaaa agttaaccct tcgacccact tcactcgcgc ttgcattttt gctactccac
    15121 tgcgtcaatt ttcctgacag agtacgcgta ctaaccaaat cgcgcaacgg aaggcgacct
    15181 gggtcatgct gaagcgagac accaggagac acaaagcgaa agctatgcta aaacagtcag
    15241 gatgctacag taatacattg atgtactgca tgtatgcaaa ggacgtcaca ttaccgtgca
    15301 gtacagttga tagccccttc ccaggtagcg ggaagcatat ttcggcaatc cagagacagc
    15361 ggcgttatct ggctctggag aaagcttata acagaggata accgcgcatg gtgcttggca
    15421 aaccgcaaac agacccgact ctcgaatggt tcttgtctca ttgccacatt cataagtacc
    15481 catccaagag cacgcttatt caccagggtg aaaaagcgga aacgctgtac tacatcgtta
    15541 aaggctctgt ggcagtgctg atcaaagacg aagagggtaa agaaatgatc ctctcctatc
    15601 tgaatcaggg tgattttatt ggcgaactgg gcctgtttga agagggccag gaacgtagcg
    15661 catgggtacg tgcgaaaacc gcctgtgaag tggctgaaat ttcgtacaaa aaatttcgcc
    15721 aattgattca ggtaaacccg gacattctga tgcgtctgtc tgcacagatg gcgcgtcgtc
    15781 tgcaagtcac ttcagagaaa gtgggcaacc tggcgttcct cgacgtgacg ggccgcattg
    15841 cacagactct gcttaatctg gcaaaacaac cagacgctat gactcacccg gacggtatgc
    15901 aaatcaaaat tacccgtcag gaaatcggtc agattgtcgg ctgttctcgt gaaaccgtgg
    15961 gacgcattct gaagatgctt gaagatcaga acctgatctc cgcacacggt aaaaccatcg
    16021 tcgtttacgg cactcgttaa tcccgtcgga gtggcgcgtt acctggtagc gcgccatttt
    16081 gtttcccccg atgtggcgca gactgattta tcaccccgat attaactatg cactacgaca
    16141 aacgctggtg ctatgtttgc ccgtggccgt tgggttaatg cttggcgaat tacgattcgg
    16201 tctgctcttc tctctcgttc ctgcctgttg caatattgcg ggccttgata cgcctcataa
    16261 acgttttttc aaacgcttaa tcattggtgc gtcgctgttt gccacctgta gcttactgac
    16321 acagctgcta ctggcaaaag atgttcccct gccctttttg ctgaccggat taacgctggt
    16381 acttggcgtc accgctgagt tggggccatt gcacgcaaaa ttgcttcccg catcgctgct
    16441 ggcagccatt tttaccctca gtctggcggg atacatgccg gtctgggaac cgttgctcat
    16501 ctatgcgttg ggcactctct ggtacggatt gtttaactgg ttttggttct ggatctggcg
    16561 cgaacaaccg ctgcgcgagt cactaagtct gctgtaccgt gaactggcag attattgtga
    16621 agccaaatac agcctgctta cccagcacac cgcccctgaa aaagcgctgc cgccgctgct
    16681 ggtgcgccag caaaaagcgg tcgatctaat tacccagtgc tatcagcaaa tgcatatgct
    16741 ttccgcgcaa aataatactg actacaagcg gatgctgcgt attttccagg aggcgctgga
    16801 tttgcaggaa catatttcgg tcagtttgca tcagccggaa gaggtgcaaa aactggtcga
    16861 gcgtagccat gcggaagaag ttatccgctg gaatgcgcaa accgtcgccg ctcgcctgcg
    16921 cgtgctggct gatgacattc tttaccatcg cctgccaacg cgttttacga tggaaaagca
    16981 aattggcgca ctggaaaaaa tcgcccgcca gcatccggat aatccggttg ggcaattctg
    17041 ctattggcac ttcagccgca ttgcccgcgt gctgcgcacc caaaaaccgc tctatgcccg
    17101 tgacttactg gccgataaac agcgacgaat gccgttactt ccggcgctga aaagttatct
    17161 gtcactaaag tctccggcgc tacgcaatgc cggacgactc agtgtgatgt taagcgttgc
    17221 cagcttgatg ggcaccgcgc tgcatctgcc gaagtcgtac tggatcctga tgacggtatt
    17281 gctggtgacg caaaatggct atggcgcaac ccgtctgagg attgtgaatc gctccgtggg
    17341 aaccgtggtc gggttaatca ttgcgggcgt ggcgctgcac tttaaaattc ccgaaggtta
    17401 caccctgacc ttgatgctga ttaccaccct tgccagctac ctgatattgc gcaaaaacta
    17461 cggctgggcg acggtcggtt ttactattac cgcagtgtat accctgcaac tgttgtggct
    17521 gaacggtgag cagtacatcc ttccgcgtct tatcgatacc attattggtt gtttaattgc
    17581 tttcggtggt accgtctggc tgtggccgca gtggcagagc gggttattgc gtaaaaacgc
    17641 ccatgacgct ttagaagcct atcaggaagc gattcgcttg attctgagcg aggatccgca
    17701 accaacgcca ctggcctggc agcgaatgcg ggtaaatcag gcgcataaca ctctgtataa
    17761 ctcattgaat caggcgatgc aggaaccggc attcaacagc cattatctgg cagatatgaa
    17821 actgtgggta acgcacagcc agtttattgt tgagcatatt aatgctatga ccacgctggc
    17881 acgggaacac cgggcattgc cacctgaact ggcgcaagaa tatttgcagt cttgtgaaat
    17941 cgccattcag cgttgtcagc agcggctgga gtatgacgaa ccggggagtt ctggcgatgc
    18001 caatatcatg gatgcgccgg agatgcagcc gcacgaaggc gcggcaggta cgctggagca
    18061 gcatttacag cggattattg gtcatctgaa caccatgcac acaatttcgt cgatggcatg
    18121 gcgtcagcga ccgcatcacg ggatttggct gagtcgcaag ttgcgggatt cgaagacgta
    18181 atacaggcta aaccatcgga tgaggtgttc acgccgcatc cgacatcttt ttcctgatgc
    18241 gtcttattta cgccccaaca actttcgcca ccgcgtgggc gaaacgttgc atcccttcat
    18301 cgatatccgc atcttccacc accagcgacg gtgcaaaacg catcacatcc ggtccggcat
    18361 tcagcaccat tacgccagcc tctgcgcccg catacaggaa atcacgcgcc cgacctttgt
    18421 actgtggctt aagctccgcg ccaatcaaca gccccatacc gcgaatatcg ctaaacacat
    18481 cgtactgctg atcgatcttc tgcaaatggt caacaaaacg ctggcgtttc gcctgaatgc
    18541 cttccagcac ttcaggtgta ttgatgatat caaacgccgc ccccgctact gcacaggcca
    18601 gaggattacc gccgtaggtg gaaccgtgag aaccaggatg aaacgcagaa gcaatttccg
    18661 ccgtggtcag catggcgcta atcgggaagc cgccgcctaa cgctttcgca gaagtcagaa
    18721 tatccggcgt cacgccgtag tgcatgtaag caaacaaatc gccagtccgc cccatcccgc
    18781 actgcacttc atcaaacacc aataatgcct gatattgatc gcacagctcg cgcaagccct
    18841 gcaaaaactc tggcgtcgct gccgtcacac cgccctcgcc ctggatcggc tcaaccacca
    18901 ccgcacaggt gtgatcatcc atcaccgctt tcactgcgtg gagatcgtta aagggaacgt
    18961 ggatgatgtc tgctggtttt ggcccaaagc cgtcggaata ttttggctgc ccacccaccg
    19021 aaacggtaaa cagcgagcga ccatgaaaag cgttatggaa ggcaataatt ttggttttga
    19081 acgggctatg acgcacacag gcgtaatggc gtgccagttt aaaggcggtt tcgttagctt
    19141 ccgtgccaga gttcataaac accacacgtt cggcaaacgt tgcctcaatc agtttacgcc
    19201 caagacgcag cgccggttca ttggtgaaaa cgttactgat atgccacaga gtttcgccct
    19261 gggtttttaa cgcgttcacc agcgcaggat ggcaatggcc caacgccgta actgcaatgc
    19321 cacccgcgaa atcgacatac tccttgcctt gctgatccca gattcggctg ccctgacctt
    19381 ttaccggaat gaactctgcc ggagcataaa tcggcaggat cacttcatcg aaagtcgcgc
    19441 gtgtaattgc tgtttgttca attgccatct catgatcacc ctgttacgca taaataaatg
    19501 tgaaattata atcacaaaat atgcataaaa aatcattgaa tggcaatcag aaatcagcga
    19561 tgtaggaaat tagccagcag ttgatgtcct tgttcgctaa gaatactttc tggatggaac
    19621 tgcacacctt ccagatccca ctggcgatgg cgaatcccca taatctcgcg ggtttcgctc
    19681 caggccgtca cgtcaaagca cgcaggtaat gagtcaggtt ccaccaccag cgaatgatag
    19741 cgtgtcacag taagtggatt tgccagcccc cgaaatacgc cctcaccgct atgtgtaatc
    19801 ggcgaggttt tgccgtgcat gacctttgca gcgcgcacaa ctttaccgcc aaatgcctgc
    19861 gccattgcct gatgaccgag gcagacgcca agaatcggca agcgcccggc atagtggcga
    19921 ataacgtcaa gggagatccc ggcttcatct ggcgtacagg ggccgggtga gatgacaatt
    19981 ttttgtggtt taagggcgtc gatatccgcc agcgtcaacg catcgttgcg cttaaccagc
    20041 acatccgccc ccagttcaca aaaatactgg tagaggttcc aggtaaaaga atcgtagtta
    20101 tctataagca ggatcatggc ggctccggta cgaaagaacc gcgctatttt actcagattc
    20161 cccggcttcg cttactactt tgctaaatat catctgcagt gcggtgagat cccccattgc
    20221 cccactctga tttgcctgat tccaggcctc tttttcaata ccctgccagc tcagttgata
    20281 tccggcatga atcgccagtt gctcgaagaa gatccgctgt gccagaccac ttcccacccg
    20341 gaagggatgc agcacgttga tttcacaata gtaatgcgcc agccgctcga cgaacttcgc
    20401 tttctccagg ccaaccagat aaccttcttc ctccagatcc tgcatcaggg cattgccctc
    20461 tttttcgata taagcaaagt ggcagaacgg cgtatcgccc tgataaatat caacttcacg
    20521 cagttgccct gcccagtcga aaatatcctg atacagctgg cgatggatag tgcgcaaatg
    20581 cggtaaaccg cgcaccagcg gaccaagctc aatggtcgca gcacgcagcg ccgtcatttc
    20641 gtaagcggcc tgttccagcc gctgctgctg gcggatgttc agccggttac gcatgatatc
    20701 aaggcctgga taaagatacg gatcgcgccc ttcgccgaat ttatcgctca tagtgcctcc
    20761 tcagctcttc aaggcgcgct aaagcctctg cggcagtaag agtgactaaa ggcatctcga
    20821 cgccttcaag acggcgactg gcctggaaat tacgattacg ctgaagctcc cagagacggg
    20881 acttttgctt atcggtgagt ttcttcactt gatgcctccc tgaacgtgtc catttgccac
    20941 aagtataagc ggcaaatccg ggttacgccg ggagagcaga agcgcgggca agattgcccg
    21001 cgcgagaaat cattacggca ggactttagc ggaaaggata actaccggtt ttgacggcac
    21061 attctggtac ggaccaacgt catgagtcgg cacctgggaa atcttatcgg caacgtccat
    21121 gcctttcacc actttaccaa ataccgcgta accgaaatca cgctgaccat ggtcaaggaa
    21181 ggcgttatcg gcaacgttga taaagaactg gctggtggcg ctgtctttgt cggcggtacg
    21241 cgccatcgcg atggtaccac gcgtgttgcg caggccgtta tcggcttcat ttttgattgg
    21301 cgggtttggt tttttctgct gcatctgctc ggtgaaaccg ccgccctgaa tcataaagcc
    21361 aggaatgacg cggtgaaagg tagtattgtt ataaaaaccg ctgttcacat aatcgacaaa
    21421 gttctgcaca gacactggcg ctttttgttt atccagctcc agttcgatgt taccagctga
    21481 ggttgtcaac aatacgtgcg ggtccccttt cgctgccatt gctgcgggag aaagagcaga
    21541 aagagcgaaa acagcagcca tcgccgccag ggtcgatttg aacatgagat ttccttaaca
    21601 aacagagcag aaaaaaagca agtgaagaga ttctaaatag ccttcaagaa caaggccatc
    21661 cctttaccta attttacgta tctgaaatat atgtaacctg acgatcactt tttattccgt
    21721 gatcaaaatc acctcttaaa atgcaattta gcaaccgatt gcaataaaac atttaaacag
    21781 atcacaaaat cacctaaaat cgcccgctcg cggcatatcc cgctatgcca ttttattttt
    21841 gaacgcaggc caatcatgac taacagcaat cgcatcaagc tcacatggat tagctttctc
    21901 tcctacgcac tgactggtgc gttggttatt gtcaccggga tggtgatggg aaatatcgcc
    21961 gattatttca atctgcctgt ttccagtatg agtaacacct tcaccttcct caacgccggc
    22021 attttaatct ctatattcct caacgcctgg ctgatggaaa tcgtcccgtt gaaaacgcag
    22081 ttacgttttg gctttctcct gatggtgctg gcggttgccg gtttgatgtt cagccacagc
    22141 ctggcgctgt tctcggcggc gatgttcatt ctcggggtgg tcagcggcat caccatgtcg
    22201 attggtacat tcctggtaac acaaatgtat gaagggcgtc agcgcggttc ccgcctgtta
    22261 tttaccgact ccttcttcag tatggctggg atgattttcc caatgatcgc cgcgtttcta
    22321 ctggcgcgca gcattgagtg gtactgggtt tatgcctgca tcgggctggt gtatgtcgct
    22381 atttttattc tgactttcgg ctgtgagttc ccggcgctgg gcaaacatgc gccaaaaacg
    22441 gatgctccgg tagcgaaaga aaagtggggg atcggcgtac tgtttctctc tgttgcggcg
    22501 ctgtgctaca tcctcggtca gttaggtttt atctcctggg tgcctgagta tgccaaaggc
    22561 ctgggcatga gcctgaacga cgcgggcacg ctggtgagta acttctggat gtcatacatg
    22621 gtcggcatgt gggcgttcag ctttattctt cgcttctttg atttgcaacg cattctgacc
    22681 gtactggctg gtctggctgc gattctgatg tacgtcttta acaccggaac accggcacat
    22741 atggcgtggt caattctcgc cctgggcttc ttctccagcg cgatctatac caccatcatc
    22801 accctgggtt cacagcagac caaagtaccg tcgccaaaac tggttaactt tgtcctgacc
    22861 tgcgggacca tcggtactat gttgaccttt gtggttaccg gcccgattgt tgaacatagc
    22921 ggtccgcagg cggcactgct tacagcaaac ggtctgtacg ctgtcgtctt tgtaatgtgc
    22981 ttcctgttag gttttgtcag ccgtcaccgt cagcataaca ccctgacctc tcattaattg
    23041 ctcatgccgg acggcactat cgtcgtccgg ccttttcctc tcttcccccg ctacgtgcat
    23101 ctatttctat aaacccgttc attttgtcca ttttttgcac aaacatgaaa tgtcagacaa
    23161 ttccgtgact taagaaaatt tatacaaatc agcaatatac cccttaagga gtatataaag
    23221 gtgaatttga tttacatcaa taagcggggt tgctgaatcg ttaaggtagg cggtaataga
    23281 aaagaaatcg aggcaaaaat gagcaaagtc agactcgcaa ttatcggtaa cggtatggtc
    23341 ggccatcgct ttatcgaaga tcttcttgat aaatctgatg cggccaactt tgatattacc
    23401 gttttctgtg aagaaccgcg catcgcttat gaccgcgtac acctctcgtc ttacttctct
    23461 caccacaccg ccgaagagct gtcgctggtg cgcgaaggct tctacgagaa acacggcatc
    23521 aaagttctga tcggcgaacg cgctatcacc atcaaccgtc aggagaaggt gattcactcc
    23581 agcgccgggc gcaccgtttt ttatgacaag ctgatcatgg cgaccggttc ctacccgtgg
    23641 atcccgccaa tcaaaggttc tgatactcag gactgctttg tctatcgtac tattgaagac
    23701 ctcaacgcca ttgaatcctg cgcccgtcgc agcaaacgcg gtgccgttgt tggtggcggc
    23761 ctgttaggtc tggaagccgc tggcgcgctg aaaaacttag gtattgaaac ccacgttatc
    23821 gaatttgccc ctatgctgat ggcggaacag cttgatcaga tgggtggcga gcagctgcgt
    23881 cgcaaaatcg aaagtatggg cgtgcgcgtt cacaccagca aaaacaccct tgagattgtg
    23941 caggaaggtg tcgaagcgcg taaaaccatg cgttttgccg acggcagcga actggaagtc
    24001 gactttatcg tcttctctac cggtatccgt ccgcgcgata agctggcaac ccagtgtggt
    24061 ctggacgttg ctccgcgtgg gggtattgtt attaatgatt cctgccagac ttccgatccg
    24121 gatatctacg ccatcggtga atgcgcaagc tggaacaacc gtgtatttgg tctggtagca
    24181 ccaggctaca aaatggcgca ggtcgccgtt gaccatattc tcggtagcga aaacgccttt
    24241 gaaggtgctg accttagcgc caagctgaaa ctgctgggcg tagacgtagg cggtattggt
    24301 gatgcgcacg gtcgcacacc aggcgcacgt agctacgttt acctcgacga aagcaaagag
    24361 atctacaaac gccttattgt cagcgaagac aacaaaaccc tgctcggtgc ggtactggtg
    24421 ggcgatacca gcgactacgg caacctgctg caactggtgc tgaatgctat cgagctgccg
    24481 gaaaacccgg attctctgat tctgccggcg cactcgggta gcggtaagcc gtctatcggt
    24541 gttgataaac tgccggacag cgcgcaaatc tgctcctgct tcgacgtcac caaaggtgat
    24601 ctgattgctg ccatcaacaa aggctgtcac accgttgcgg cgctgaaagc cgaaaccaaa
    24661 gcgggtactg gctgcggtgg ctgtatcccg ctggtcactc aggtactgaa cgcggaactg
    24721 gcgaaacagg gcatcgaagt taacaacaac ctgtgcgaac actttgctta ttcgcgtcag
    24781 gaactgttcc atttgatccg cgttgaaggc attaaaacct tcgaagaact gctggcgaaa
    24841 cacggcaaag gttacggttg tgaagtttgt aaaccgaccg tcggttcgct gctggcctcc
    24901 tgctggaacg aatacattct gaagccggaa catactccgc tgcaggattc taacgacaac
    24961 ttcctcgcta acatccagaa agacggcacc tactcggtga tcccgcgctc tccgggcggt
    25021 gaaatcaccc cggaagggct gatggcagtg ggtcgtatcg cgcgtgaatt taatctctac
    25081 accaaaatca ccggctccca gcgcctggcg atgtttggcg cacagaaaga cgatctgccg
    25141 gaaatctggc gtcagctgat tgaagccggt ttcgaaaccg gtcatgccta tgcgaaagca
    25201 ctgcgtatgg cgaaaacctg cgtgggtagc acctggtgcc gctacggcgt gggcgacagc
    25261 gtgggcctcg gcgtggaact ggaaaaccgc tacaaaggca tccgtacgcc gcacaaaatg
    25321 aagttcggtg tctccggctg tacccgtgaa tgttcagaag ctcagggtaa agacgtgggt
    25381 attatcgcca ctgaaaaagg ctggaacctg tatgtttgcg gtaacggcgg catgaaaccg
    25441 cgtcatgcgg atctgctggc ggcggatatc gatcgcgaaa cgctgatcaa atatctcgac
    25501 cgcttcatga tgttctacat ccgtaccgcc gacaaactga cgcgtaccgc gccgtggtta
    25561 gaaaacctcg aaggcggcat cgattacctg aaagcagtga tcattgacga caaactgggg
    25621 ctgaacgcac atctggaaga agagatggcg cgcctgcgtg aagcggtagt gtgtgagtgg
    25681 actgaaacgg tcaatacgcc gtctgcgcag actcgcttca aacacttcat caacagcgac
    25741 aaacgtgacc cgaacgtaca gatggtgcca gagcgcgaac agcaccgtcc ggcaacgccg
    25801 tatgaacgta tcccggtaac tctggtggag gacaacgcat gagccagtgg aaagacatct
    25861 gcaaaatcga tgacatcctg cctgaaaccg gcgtctgcgc gctgttaggt gacgagcagg
    25921 tcgcgatttt ccgcccgtat cacagcgatc aggtgtttgc gatcagcaac atcgacccgt
    25981 tcttcgagtc cagcgtgcta tcacgcggac tgattgcgga acaccagggc gagctgtggg
    26041 tcgccagccc gctgaaaaaa cagcgttttc gcttaagcga cggcttgtgc atggaagacg
    26101 aacagttttc cgtcaaacat tacgaagcgc gagtgaaaga cggcgtggtg cagctgcgcg
    26161 gttaatgttt taacgggagg cgcaatgcct cccctttttg catggtcctg taataatctt
    26221 cggtatattg caggacattt tttaaacttt ttgttttatt ttttgttttt attttttaaa
    26281 ggataatcaa atgtttacag acactattaa taagtgtgcg gctaacgctg cgcgcattgc
    26341 acgcctgtcg gcaaataacc cgctcggctt ttgggtcagc tccgccatgg cgggcgcgta
    26401 tgtgggtctt gggatcatcc tgatttttac gctcggtaat ttgctcgatc catccgtacg
    26461 ccctctggtg atgggcgcga cctttggtat cgccttaacg ctggtgatta tcgccggttc
    26521 tgaactgttc accggacaca ccatgttcct cacctttggg gtgaaagcgg gcaccatcag
    26581 ccacgggcaa atgtgggcaa tcctgccgca aacctggctg ggtaacctgg tcggttccgt
    26641 cttcgttgcc atgctttata gctggggggg cggtagcctg ctgccggtag ataccagcat
    26701 cgttcactcc gtcgcgctgg ctaaaaccac tgcaccggca atggtactgt tcttcaaagg
    26761 tgcattgtgt aactggctgg tttgtctggc aatctggatg gcgctgcgca ctgaaggggc
    26821 ggcgaaattt atcgcaatct ggtggtgtct gctggcattt atcgcgtccg gctacgagca
    26881 ctccatcgct aacatgacgc tgttcgcgct ctcctggttc ggcaaccaca gcgaagccta
    26941 cacgctggcg ggtattggtc ataacctgct gtgggtgacg ctgggtaata ctttatcagg
    27001 tgccgtattc atgggattgg gttattggta tgctacgccg aaagcgaatc gtccggttgc
    27061 ggacaaattt aatcaaactg aaacggctgc cggttaatta ctaaggggtt tttacgtgga
    27121 tcatttgcct atattttgcc aattacgcga tcgcgactgt ctgattgtcg gcggtggtga
    27181 tgtcgcggaa cgcaaagcaa ggttgctgtt agacgcaggc gctcgcttaa cggtgaatgc
    27241 attagcgttt attccacagt tcaccgcatg ggcagatgca ggcatgttaa ccctcgtcga
    27301 agggccattt gatgaaagcc ttctcgacac ctgctggctg gcgattgcag cgacggatga
    27361 tgacgcgctt aaccagcgcg tcagcgaagc cgctgaagct cgtcgcatct tctgtaacgt
    27421 ggtcgatgcg ccgaaagctg ccagcttcat tatgccgtcg attattgacc gctcaccgct
    27481 gatggtggcg gtttcctctg gcggcacctc tccggttctg gcacgcctgt tgcgcgaaaa
    27541 acttgaatca ctgctgccgt tacatctggg ccaggtagcg aaatacgccg ggcaattacg
    27601 cggtcgagtg aaacaacagt tcgccacgat gggtgagcgt cgccgtttct gggagaaatt
    27661 gtttgttaac gaccgcctgg cgcagtcgct ggcaaacaac gatcagaaag ccattactga
    27721 aacgaccgaa cagttaatca atgaaccgct cgaccatcgc ggtgaagtgg tgctggttgg
    27781 tgcaggtccg ggcgatgccg ggctgctgac actgaaagga ctgcaacaaa ttcagcaggc
    27841 agatgtggtg gtctacgacc gtctggtttc tgacgatatt atgaatctgg tacgccgcga
    27901 tgctgatcgc gttttcgtcg gcaaacgcgc gggataccac tgcgtaccgc aggaagagat
    27961 taaccagatc ctgctgcggg aagcgcaaaa aggcaaacgc gtggtgcgtc tgaaaggtgg
    28021 cgatccgttt atttttggcc gtggtggcga agagctggaa acactgtgca acgcgggtat
    28081 tccgttctcg gtggttccgg gtattaccgc agcttctggt tgctctgcct attcaggtat
    28141 tccgctcacg catcgcgatt atgcccagag cgtacgctta attaccggac acttaaaaac
    28201 cggtggcgag ctggactggg aaaacctggc ggcagaaaaa cagacgctgg tgttttatat
    28261 ggggctgaat caggctgcga ctattcagca aaagctgatt gaacacggta tgcctggcga
    28321 aatgccggta gcaattgtcg aaaacggaac agcagtcacg cagcgcgtga ttgacggtac
    28381 gctcacacaa ctgggcgaac tggcgcagca aatgaacagt ccatcgctaa ttattattgg
    28441 tcgggttgtt ggcctgcgcg ataaactgaa ctggttctcc aaccattaat ttaatcaggg
    28501 ccagagaatt ctggcctttt tcgtttacaa ctctctttat atattcagca gataaaaaca
    28561 taacccctta ataaatatta tctgctaatg ggttatattc accgttacag cgacttacaa
    28621 aattaagtca ttcactcact gacgccgatt aaatattcct gtatcgt
//
LOCUS       DAZFKF010000061        28641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFKF010000061 DAZFKF010000000
VERSION     DAZFKF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.061"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(216..291)
                     /locus_tag="ACHKA1_003125"
     tRNA            complement(216..291)
                     /locus_tag="ACHKA1_003125"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(256..258),aa:Asn,seq:gtt)
     gene            complement(392..1879)
                     /gene="yeeO"
                     /locus_tag="ACHKA1_003126"
     CDS             complement(392..1879)
                     /gene="yeeO"
                     /locus_tag="ACHKA1_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO5680739.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKEAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
     gene            2040..2115
                     /locus_tag="ACHKA1_003127"
     tRNA            2040..2115
                     /locus_tag="ACHKA1_003127"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2073..2075,aa:Asn,seq:gtt)
     gene            complement(2153..3103)
                     /gene="cbl"
                     /locus_tag="ACHKA1_003128"
     CDS             complement(2153..3103)
                     /gene="cbl"
                     /locus_tag="ACHKA1_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO5680740.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGIVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3205..4122)
                     /gene="nac"
                     /locus_tag="ACHKA1_003129"
     CDS             complement(3205..4122)
                     /gene="nac"
                     /locus_tag="ACHKA1_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO5680741.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4448..4523
                     /locus_tag="ACHKA1_003130"
     tRNA            4448..4523
                     /locus_tag="ACHKA1_003130"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4481..4483,aa:Asn,seq:gtt)
     gene            complement(4579..5514)
                     /gene="ldtA"
                     /locus_tag="ACHKA1_003131"
     CDS             complement(4579..5514)
                     /gene="ldtA"
                     /locus_tag="ACHKA1_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5680742.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5576..6655)
                     /gene="cobT"
                     /locus_tag="ACHKA1_003132"
     CDS             complement(5576..6655)
                     /gene="cobT"
                     /locus_tag="ACHKA1_003132"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO5680743.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6667..7410)
                     /gene="cobS"
                     /locus_tag="ACHKA1_003133"
     CDS             complement(6667..7410)
                     /gene="cobS"
                     /locus_tag="ACHKA1_003133"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO5680744.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLMALL"
     gene            complement(7407..7952)
                     /gene="cobU"
                     /locus_tag="ACHKA1_003134"
     CDS             complement(7407..7952)
                     /gene="cobU"
                     /locus_tag="ACHKA1_003134"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO5680745.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRKGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQVINTEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            8176..8310
                     /locus_tag="ACHKA1_003135"
     CDS             8176..8310
                     /locus_tag="ACHKA1_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO5680746.1"
                     /translation="MEEISLVKVWLREAGAHNVLVTSAVNNNGVAELLALLHTEEGCR
                     "
     gene            complement(8411..8740)
                     /gene="tmaR"
                     /locus_tag="ACHKA1_003136"
     CDS             complement(8411..8740)
                     /gene="tmaR"
                     /locus_tag="ACHKA1_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO5680747.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(8912..9970)
                     /gene="yeeA"
                     /locus_tag="ACHKA1_003137"
     CDS             complement(8912..9970)
                     /gene="yeeA"
                     /locus_tag="ACHKA1_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO5680748.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRAVRK"
     gene            complement(10169..10642)
                     /gene="sbmC"
                     /locus_tag="ACHKA1_003138"
     CDS             complement(10169..10642)
                     /gene="sbmC"
                     /locus_tag="ACHKA1_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO5680749.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(10761..11927)
                     /gene="dacD"
                     /locus_tag="ACHKA1_003139"
     CDS             complement(10761..11927)
                     /gene="dacD"
                     /locus_tag="ACHKA1_003139"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO5680750.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            12136..13563
                     /gene="sbcB"
                     /locus_tag="ACHKA1_003140"
     CDS             12136..13563
                     /gene="sbcB"
                     /locus_tag="ACHKA1_003140"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO5680751.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNLIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGNNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(13606..13833)
                     /gene="tsuB"
                     /locus_tag="ACHKA1_003141"
     CDS             complement(13606..13833)
                     /gene="tsuB"
                     /locus_tag="ACHKA1_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO5680752.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(13847..14905)
                     /gene="tsuA"
                     /locus_tag="ACHKA1_003142"
     CDS             complement(13847..14905)
                     /gene="tsuA"
                     /locus_tag="ACHKA1_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO5680753.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASSLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(15085..16443)
                     /gene="plaP"
                     /locus_tag="ACHKA1_003143"
     CDS             complement(15085..16443)
                     /gene="plaP"
                     /locus_tag="ACHKA1_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO5680754.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(16433..16495)
                     /gene="yoeI"
                     /locus_tag="ACHKA1_003144"
     CDS             complement(16433..16495)
                     /gene="yoeI"
                     /locus_tag="ACHKA1_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO5680755.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(16710..17639)
                     /gene="yeeY"
                     /locus_tag="ACHKA1_003145"
     CDS             complement(16710..17639)
                     /gene="yeeY"
                     /locus_tag="ACHKA1_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5680756.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANCAIAGVYAKSDDGKSAI"
     gene            complement(17685..18509)
                     /gene="yeeZ"
                     /locus_tag="ACHKA1_003146"
     CDS             complement(17685..18509)
                     /gene="yeeZ"
                     /locus_tag="ACHKA1_003146"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5680757.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            18936..18986
                     /gene="hisL"
                     /locus_tag="ACHKA1_003147"
     CDS             18936..18986
                     /gene="hisL"
                     /locus_tag="ACHKA1_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO5680758.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    18962..19085
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            19132..20031
                     /gene="hisG"
                     /locus_tag="ACHKA1_003148"
     CDS             19132..20031
                     /gene="hisG"
                     /locus_tag="ACHKA1_003148"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO5680759.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            20037..21341
                     /gene="hisD"
                     /locus_tag="ACHKA1_003149"
     CDS             20037..21341
                     /gene="hisD"
                     /locus_tag="ACHKA1_003149"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO5680760.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSTLASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            21338..22408
                     /gene="hisC"
                     /locus_tag="ACHKA1_003150"
     CDS             21338..22408
                     /gene="hisC"
                     /locus_tag="ACHKA1_003150"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO5680761.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLMAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            22408..23475
                     /gene="hisB"
                     /locus_tag="ACHKA1_003151"
     CDS             22408..23475
                     /gene="hisB"
                     /locus_tag="ACHKA1_003151"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO5680762.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            23475..24065
                     /gene="hisH"
                     /locus_tag="ACHKA1_003152"
     CDS             23475..24065
                     /gene="hisH"
                     /locus_tag="ACHKA1_003152"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO5680763.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            24065..24802
                     /gene="hisA"
                     /locus_tag="ACHKA1_003153"
     CDS             24065..24802
                     /gene="hisA"
                     /locus_tag="ACHKA1_003153"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO5680764.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSAEDVAALLEAGVARVVVG
                     STAVKSPDMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            24784..25560
                     /gene="hisF"
                     /locus_tag="ACHKA1_003154"
     CDS             24784..25560
                     /gene="hisF"
                     /locus_tag="ACHKA1_003154"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO5680765.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDEGRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            25554..26165
                     /gene="hisIE"
                     /locus_tag="ACHKA1_003155"
     CDS             25554..26165
                     /gene="hisIE"
                     /locus_tag="ACHKA1_003155"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO5680766.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPKALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGETAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(26262..27239)
                     /gene="wzzB"
                     /locus_tag="ACHKA1_003156"
     CDS             complement(26262..27239)
                     /gene="wzzB"
                     /locus_tag="ACHKA1_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO5680767.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETAEGAQKQLAKYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(27386..28552)
                     /gene="ugd"
                     /locus_tag="ACHKA1_003157"
     CDS             complement(27386..28552)
                     /gene="ugd"
                     /locus_tag="ACHKA1_003157"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO5680768.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         7535 a         7515 c         6819 g         6772 t
ORIGIN      
        1 tgtacggtac ttcatggaac agaatttttg accttgtgaa tcgtgatgtc tgttggggag
       61 gggcaatttg ctcactgaag cgtgagactc gattaagcgc acgaaacaca gaaatcaaaa
      121 aacccggtca ctttttttta caaagtgacc gggtaaaaat aatttttatt ttttaactgt
      181 tttgagactc atagagatgt ctcaaagcta aaatttggct cctctgactg gactcgaacc
      241 agtgacatac ggattaacag tccgccgttc taccgactga actacagagg aatcgtgaga
      301 acgaggcgaa tattagcgat gcccacccac aatgtcaaag cctgtttttt aaatttgaaa
      361 tcgtttgctg aaataatctg cattttgtcg tttattccga cacagctggc tttttttcac
      421 acttttgcgg ctcggatcga gggtatttcc atagccaacg tccagtaacc attcgccagt
      481 aaaacagcac ggcccgcaca gcccagtcgg caaacattcc catccagaca ccaaccacac
      541 cccagccaag catgattccc agcacataac cgacgacaac ccgacaaccc cacatgctca
      601 acatcgaaac ccacatggcg taacgggcat cacgagcacc tttaaatcca gcgggtagca
      661 cccatgaggc ggaccaaata ggcataaata aagcatttag ccaaatcaga atcacaacga
      721 catgtttaac ctgtggatcc tgggtgtaaa acgatgccat aaccccggca aagggagccg
      781 ttagccaggc gatggccgtt aatccaagag tggacagcca gaacacatgc cgcaactgaa
      841 tctctgcttg cgctatctgc cctaccccca accttcggcc tgtaatgatc gtagaagcag
      901 agccgagcgc acttccgggt aagttgataa gagccgcaat tgaaaacgcg ataaaatttc
      961 cggcaataac actggtcccc atcccggcaa cgaacatttg ggttaataac cgaccactgg
     1021 taaataacac tgattcgaca ctcgctggaa taccaatccc catgacttcc cagataatgc
     1081 taaaattcag cggtttaaaa tagctcttta acgaaatcct tagcgcagga ttaaaaccaa
     1141 tcgccagcac ccacaaaatt gcaactgcgc caatataacg agaaatggtt aaacccaacc
     1201 cagccccgac aaatcccagt cccggccagg agaaaaggcc gtaaatcaat atgccgctaa
     1261 taataatatt aagaatattc aggctaccgt taatcagtag cggtattttc gtattccctg
     1321 caccacgaag tgccccgcta ccaattagag tgatggcagc tgctggataa ctgagtaccg
     1381 tcagctccag ataagtcaac gccagtgctt taacttctgt cgtggcatca cccgcgacga
     1441 aatcaataat ttgctcgcca aaatgatgaa taagcgttgc caacagtacg gcaaacaacg
     1501 tcatgatcac caatgactgc cgcgtcgcca ccctcgctcg tcgtcgatcc cgcttaccga
     1561 gactaaatgc cacaacgaca gtagtaccaa gatcgatagc agcaaaaaaa gccataatga
     1621 ccatattgaa gctgtccgcc aatcccacgc cggccatcgc ttcttttccc agccagctga
     1681 ccagaaaagt gctcagaacc cccatcaaca ggacacaggc attctccatg aagataggaa
     1741 cagcaagcgg ggttatctcg cgccagaaca acactttgta gctcttgcgt ttagcgtgcc
     1801 agcgagtgcc gtgaacaacc tggcgtaaag cagaggagat attcaaagcc gaccttaatt
     1861 gcagaaagtg aaaccacatt tcaaataatg agggagaatc agcaaagctg caaagatttt
     1921 cgccaacaaa ttgtctgcaa atgcaacaaa ctgttgatag aaacggcaaa cagttgggga
     1981 atttaaaaat cgggtttgac aaaagatttt tcgccgttaa gatgtgcctc aacagcgatt
     2041 cctctgtagt tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc
     2101 cagtcagagg agccaaattt ctgaaaagcc cgcttttata gcgggctttt tgctatatct
     2161 gataatcaat ttcctcttca ctgttttcca tcacctgccg cttgatatcc tcaactgaaa
     2221 gtcctgcatt acaaagttcc agaaagcgcc agacatagtt acgctgaagt tgtcctcgct
     2281 tcagtcccaa ccagacagta ttagcatcaa aaagatgccg cgtatccagg cggattaaat
     2341 tcttttcctc ttgttcaccg ctggattgtt cggcaactat tccgatccca agcccaagag
     2401 caacataggt tttaatgaca tcagaatcct gcgcacttaa tacaatatct gccagcaaac
     2461 ctttgcgggc aaatgcgtca tcaatacgtg agcgccccgt aatcccctgt cggtaagtga
     2521 ttaacggcca cttcgctatt gactccagcg tcaatggtgt aatttgcgtc aagggatgat
     2581 cgagtggaac aagcaaacta tggtgccaac gaaaccacgg gaaggcgacg agctgcgggt
     2641 cattactcaa acgctcgctg gcgataccaa tatcagcttc accattatgc aacaacgtcg
     2701 caatttcctg tggcgtcccc tggattagct cgagccgaac ctccgggaaa agttcgcgaa
     2761 aagctttaat gacctctggc aagctataac gtgcctgagt atgcgtcgtt gcaatagtga
     2821 gaacgccaga cgtatcgttg gtaaacaggt ctgcaagccg acgaacatta ctggcttcat
     2881 tcagaatacg ttctgcaatg accagtaatg ctttgcccgg ttcagtcatg cccagcagtc
     2941 gcttacctcg tcgaacaaat atttcgatgc caagttcatc ctccagttcc cgaatatgac
     3001 ggctgacgcc tgactgtgag gtaaaaagca tattcgcaac ctctgtcagg ttgtaatcct
     3061 gacgtgcagc ttcgcggatt atctttagtt gttggaaatt cacggtaaac tccgggcagt
     3121 tcagatattc cgttattgtt aaagtctaac gcccggcata acaaataata aaaacccgca
     3181 tcttattcca tcccgatata acgcttagct caccaattgc cactgccttt tttccatcac
     3241 tggagaacta atcactgaca ttaacaactc tttcactgcc tgtgcctgtg gcgataagtt
     3301 cgctctggcg ggtaaattta atgacaaaga gagactcatg gaaggcgtgg taatacgtga
     3361 catccaccca tttactgcgc cacataacga acgcgcggcc gattcgggta atactgcaac
     3421 gcccatgccg ctggcaatcg ctgcggtaag cgtggcaata gactcaattt caccaatgac
     3481 ttttgccgtg agtcgccgta gggaaaacgc ctcatcaaca cgaagtctaa cagcgctgta
     3541 atcactgggg agaaagaggt tcatttgtgc aatagcatta acatcaacgc tttgccccgg
     3601 gcaatcttga gttcctacca ggaaaagatc ttctttcagc aaagcctgac tggatacccc
     3661 agccacaggg gaatgctcat aaatcaccgc catatcgagt tggtgattta tcagtttttc
     3721 gttaagcact gcaccgctat tttcatgaag atagataacg atctccggga attcagcgcg
     3781 aaccgcttgt aataagggca tggtgataga tgacgcagcg gttcctggcg caaagccaat
     3841 cgagacttgc cccgataatg cctgaccaac gttatgcacc gccagttggg cctgctcaca
     3901 ctgacgtaaa atggcccgcg catgggtata gagaattttt ccggcgtctg ttggcgtaac
     3961 gccccgtttt gtgcgaatca aaagttgttg atttaactca ccttccagtg tggcaacctg
     4021 ctggctgagc gctggttgtg cgatatgcaa tacttcagca gcctgagtca ggctaccaat
     4081 atctacaatt tttacgaagt atttcaggcg tctgaagttc atgttgcctc cggtttttga
     4141 gaatcggccc aagtgccgcc attacttaca accagactgc aagatgcttg ccagttttat
     4201 tttggcgttg atgtacaagc taaccaactg tcaaataaga gattatgata gattcgtcat
     4261 tggctctttt caccagcagt cgcgttcccc tgccccataa aaggagagta tgcaccacga
     4321 tggttcataa ccaataagat tgaaagctca ccactttgtt gaaattgaca gcaaacaaac
     4381 aaaaaaatgc atttcaccct ttgacatcac catgcactgc cattaatatg cgccccgttc
     4441 acacgattcc tctgtagttc agtcggtaga acggcggact gttaatccgt atgtcactgg
     4501 ttcgagtcca gtcagaggag ccaaattcaa aaaagcctgc tttctagcag gctttttgct
     4561 ttctaattac caacgctctt aaaacatctg tcttgaacca gaactaattt gcacaggcat
     4621 tcccgatcga cgttgcaacg cagcatttgc gcgattcaca tcaacttctt gcccgttgat
     4681 aaacgcccgc aaagatgggg ttaccggcaa tggcactttt cggtcagact catattctgc
     4741 acgattgcgc gacagcggct catgaacttc cagccagttc gagccatctg gttcagtggt
     4801 gtattttact ggctggtcga taatttgcac acgcgtccca acaggaacat tatcaaacag
     4861 atatttgata tcgtcattgc gcagacgaat acagccctga cttacccgga gcccaatacc
     4921 aaaattggca ttggtaccgt ggatggcata caacctgcca atataaatcg cgtacagccc
     4981 catgggatta tcgggccccg caggaacaaa tgcgggcaaa ctctcccctc gtttcgcata
     5041 ttcgcgccga gtgttcggcg ttggcgtcca ggttggagct tcttgtttac gttcaacggt
     5101 agtcacccag ttacgcgggg tttctcgccc agcctggccg ataccaatag gaaagacttc
     5161 cacagtatta ctgtctggtg ggtagtaata aagacgcatc tcagcgacgt taaccacaat
     5221 ccctttacga acagtggcgg gcaaaatcag ttgctgcgga atggtgagtt gcgagccaga
     5281 cttcggcaag aaaacatcag cgcccgggtt cgcttccagc atgttactta acccttgccc
     5341 gtattgtgcg gcaaaagtct ccagcggctg ggtattgtga tcaggaacag ttacagtaaa
     5401 cgactgcccc actaaacggc taccctctgg aggtaatgga taagttaccg ccaggctagt
     5461 atggctggca aaaagcagag caaatgagca aagaatattt acacgacgca tcatgtccct
     5521 ttcctatgtc gcgaaagcta tccgttaagt atagctttta tcagactttt cgtttttaac
     5581 tgttcaaatc agaagtcgta ttccccggta gaacaatatt actggcagca agttcgccca
     5641 tgttgttgta tatcgcacag gcagcttcga tgatgggcat cgccagagct gcgccactcc
     5701 cctcacctaa acgcatctcc atattgagat aaggctccag ccccaaatgc gacagcgcta
     5761 tacgcgcccc tttttctgcc gacaggtgag aaggaatgag atacggtttg attgcaggag
     5821 acatctggca ggctgcgagc gcagcagcat aagaaagaaa tccatccagc aacacgggta
     5881 aaccgcagga agcagcgcct aacatcacgc cagccattcc gaccaaatca aatccaccca
     5941 cttttgccag aacatcaacg ccatcctgcg gatttggttg attcaacgta atcgcccggc
     6001 gcacaacatc aattttatta gccagtttat ctgtcggcag gtttgcgcca atcccaacca
     6061 cttcttcagg atcccggcca gtgattgtgc tgactattgc tgctgccggt gtcgtgtttg
     6121 ccatccccag ttcacctaca ccaaacagcg tgacaccgtt ttttgccagc tcccgcgtat
     6181 aacatatgac gtccaaaagc aacttttcag cctgacggcg actcattgcc ggagctgaag
     6241 caatattgcc gctacctcgt gcgacacgca tgttgataag cccggggata ggctcagcag
     6301 tatcaatacc aacatcaatt acgtggacgt tagcacccgc ttgtgctgcc agcacacaca
     6361 cgccggttgt tccgcgggtc atattttcag cctgaatggc tgtcacttct tttggggaaa
     6421 tagcgacccc ttcctcccag acgccgtgat cggcacacat aaccagtacc gcttttttgc
     6481 ccacatgcgg tatgccattc aaccccggca ttcctgccag ttgtatggca agcgcctcca
     6541 gccttccaag gctaccaaca ggtttgagta acccgtcaat atgccgttgt gcacgcgaca
     6601 tagcggcagg atcgatagca gggatcgtat tcagtaaatc ggcaagtgtt tgcatgtcac
     6661 gtccctttat agcagtgcca tcaggaagac cagttcacca agctctatgg ctgctcccag
     6721 cgtatcgccg gtttgcccgc ccagcgtacg tttgagaagc tgaccgagga tgaaaatcgc
     6781 caccatcgtc accaccatag cagccacacc atgcataccc ggcaataata cggcagcaaa
     6841 aatagcggct aagccgagtg tgacgcaggt ttgtcgccca tcaattttgc caatgaacac
     6901 attgccaagt ccttcttccc gggcataacg atgacgatac atcaataatg cggcagtccc
     6961 gcggctgacc gcacatgctg ccgccagcga tgcaaggatc ggttcgccac gcagagccaa
     7021 ctcgcttaat accagaatct ttgccagtac aacaaaaatc aatgccagac cgccgtgggt
     7081 gcctaaacgg ctatcacgca tgatttccaa catgcgatcg cggctacgtg cagaaaatac
     7141 gccatcacag gtatcggcga ggccgtccag gtggaatccc ccggtcatca gcgccagcac
     7201 aagcacgcta aacagtgccg ccagtggtac gccacaccat gcctgcagca ccatgaaaac
     7261 cagcccgcta atcgcgccaa gcaataatcc aattaaagga aaagtaataa taccgcgaga
     7321 ataatgctcg aaatccagtc cctgggacca gcgacgcggt acaggcagac gcgtaataaa
     7381 tgagagcatc gcccaaaata atttactcat ttgattttta ctccaatacc cgaaaccacc
     7441 agccatactt catttgccgc agcggccagc tgctgattta cccgcccggc aatatcacga
     7501 aaatgacgtg ccagacgact ctccggcaca atccccattc ccacttcgtt agtcactaat
     7561 acaacctttg cggggcaacg ttggcaggca gcaatcaacg actgaatctc agtattaatc
     7621 acctgttcca tcgcctgata atcccattca tcagggtctt tatcgccgcc ataatcaaac
     7681 aacagattag tcaccattgt ggtaacgcat tccagcaaca cagcttcatg cgggttaatg
     7741 tctgcatgaa ttaattcatc aagatgttgc caacgctcca ccgtgcgcca gtgcgccggg
     7801 cggcctttcc gatgatgctc tatccgtgca gccatctcat catcaaggat ttgggaggtg
     7861 gcgatataca gaacctgtga agagtcccca ataagcgcct ctgcgtggcg acttttcccg
     7921 ctccgtgcgc cccccgtcac caaaatcatc ataccgactc ctgattcaat tgtatgatgg
     7981 tattcattga acagaacaat ggatccccca ccgttgctga ccgcaaaaca tttacacgat
     8041 taatactgcg cgtaatataa ttaaaaatcc agcattctca atccattacg aaagaagatg
     8101 gcattgtgga atagccagac ttcctcgtgt taacgtccga tcattggagt agtcaccaaa
     8161 gcagatttag ccagcatgga agaaatatct ttggtgaaag tttggttacg ggaagccgga
     8221 gcgcacaacg tgttagtaac cagtgcggtt aataacaatg gagttgcaga acttttagcc
     8281 ttgctgcata cagaagaagg ctgtcgctaa tttcgacagg ttctcagatg agaatgatga
     8341 ctttgataca cagaaaagaa tcaatgagca atgagggttg ccgggcaacc ctcattgaat
     8401 aaaacaggaa ttacttcgct tcgccgtttt tcatttcgcc catagctttc agctttttgg
     8461 agatatcgcg gcgttctttg gagagctcgg catttttgat gatgtaatcg tcaacacgat
     8521 cttcatagtc acctttcatg ctggcgatga tgccctggat tgcttcaacg ctcatccccg
     8581 gcttgatgta atcgctcagg ttgtccagca gcaggacgcg cttctggttg tcacggatct
     8641 ttttctcaac gtcctgaatt tcacgttgca gtttgttctt acgacggaac agacgaacaa
     8701 attccagtac gtcctggaat gaaggcttgg tagtttccat ttttataccc ctgataatgt
     8761 gagagtcgga ttcgtttaag caaccgctat tcgttagggc caacattact catagctgcc
     8821 gcagatgaca atgcttttat cccttttcac tatcataccc tttatccttg ctgaatcgaa
     8881 gcagcagcaa gatgattctg aagttcagga attatttgcg cacggcccgg caaatcagat
     8941 tcgatagcaa ctcaagctga tgtgccgttt ccatgttcag ccacacataa ccatagattg
     9001 gtgtttccac aaccttcagg tcatggtggt tattgagcaa ctgacgcagc tcttccacag
     9061 catcgttcaa tttttccgta ttggcaaaaa ccggctgcgg attaccttcg tacagcgcat
     9121 gaacaaggct cagcagtatt tgctgcatca tgtgctgggt atcacgaagt ttttgcgcgt
     9181 tcaataacac gaaatggctg gggcgcgtgg cccagtacgc attgatttgc aactccagca
     9241 tacaaaccag attgcgatta attgtctgga taccttcata tatcgatttt ggaatacggg
     9301 tttctttgct ggcgggcgca atcaacccac gcattttcac ggcatcggtc aggagttttt
     9361 gtagatggct ttccagacgt gggcgttcga gtaagttcgg tgagaatgca gattgataga
     9421 cccgattata ctcggtcaga cttttcgcca gttgaatgcg ccagtggatg aacgcccgtt
     9481 gtggccagat accggtaaac aacattgcca gtaaagagcc gaggatcaca tcgccgcttc
     9541 gccataacgc cgtatcaatt tcacctgtcg gggaaccaac aacaattgcc agcgtcaccc
     9601 caatcaataa accttgatac ggtttcttgc ccagcgccag ccaaccgcaa aggaacatgg
     9661 ccgccgcgca ccagactaac atcagcggta acgagattaa ctccagttgc agagcgataa
     9721 gacctaaaat cgaacccaac accgtaccgc caatacgttc gaaggcgcga gggacaacgt
     9781 taccccagaa cgagattggc cccataatca ccaccatggt gaccagcggc caggtgcttt
     9841 ccgggatagt aaacaggcgg ataatgagaa aagtgagcag gaatgccagc gcgacccgag
     9901 taccatgcac aatgcggtaa tggcggtata cccggatttc aaacgggctt aatgacttat
     9961 cggcacgcac gcagaaactc cgtaatgaag attaaaagcg ctcataactc atctgtcggc
    10021 cagaaggcgc gcctctgaat tatccgacgg acaaaccggg aacagtgtaa tttcccggca
    10081 ggagcgaaaa aattgaaagg cgcatcttac tcttttcgct ttcaaaaaaa agagtggtca
    10141 tcgcgttaac acaccgccct gagatgaatt agtgatgttt tggctgcacc gcaacataca
    10201 tttcgatatc ccagtaccca tcttccgcgc cattgttcaa ataaacctcg aagcagggct
    10261 ttggtaacat ttcataagca ctgtcctgca agaggctatt aaagaactga taccagggtt
    10321 tagcaaaatc atcaccgact acacgagcta ccgccaccgc atactgacca cctgaaattt
    10381 ctgtcagaat gacgccctca ctgttttcgg ggagcgtaaa gtaacccggc accgtcacga
    10441 cggtgtcgca gcgtaatttt tcggcgggtg tttcatctgg attgtcgtaa tagacagcaa
    10501 cccattcctt cggcacaata tttttgctat ctacccacat catcaactgc tcaaaacctt
    10561 tctttaccgt ctgttcccac gggccaacga gatggaaacc tgcaacggta cgtttatctt
    10621 cctgcttaat ctcgtagttc atgacgcctc cattaacact gtttttatat acagtatagt
    10681 tgcaatatta aaaccacaag gaatgagtgt tgattatgcg agcagactcg cactcctgcc
    10741 agtctgctgc aaaagaaagg tcaggcctta tggtggaaat aatcactcag gcgggaaaac
    10801 atgccgcctt ccccgacaga ttccagggta accagcggcc agtgcgccac ctgtttatca
    10861 cggtcgtaaa gttcaatttc ccctacccgc tgatgggcgc taattggcgc ggtgagctct
    10921 ttaccatcaa gggtatattt ggctttgata tgtggaattt cggctttcgg tagcaccatc
    10981 cagaactctt gttccgttcc caggtcgata ttttctttat cgccatacca gatgcgttcc
    11041 gttccgacct ttttcccacg gtgcaaaatt tgcaccgtag taaagttttg ttgcccccaa
    11101 cgtagtaatt ttcttgcctc ttcctcacga ccttttgcac tgtcagcccc cattaccacg
    11161 gcaatgagac gacgctgccc atctacagcc gaagcaatga gattaaaccc ggcaccagaa
    11221 gtatgacccg ttttcaggcc gtcaacattc atggttttat cccacaacaa cccgttacgg
    11281 ttttgctggg tgataccgtt ccaggtgaga cttttctcac tgtacatatg ataaaactcg
    11341 ggctcgccgt ggatgatagc gcgagaaagt acagctaaat cataagccga gctatgctga
    11401 cctggcgcat ccaggccatg cactgtttca aaatgcgtat ccttgagatg cagcttctcg
    11461 gcatagttgt tcatcatttc aacaaactgc cgttgcccac cggcaatata gtcagccagt
    11521 gcaacacaag cgtcatttcc ggaatccaca attaaaccac ggcttaaatc acgtaccgat
    11581 acgcgatcgc cctctttcaa aaacatcagt gaagaaccga caaacaccgg attatctttc
    11641 gcccacgcat cgcgccccac ggtgacaata tcgtctggcg taatgcgatg actatcgata
    11701 gcgcgatcca cgacataacc cgtcatcagc tttgtcaggc tggcgggatt gcgctgttga
    11761 tgctcattac ccgcggtgag gatctgtccg gtggtgtaat ccatcagtac ccaggacccg
    11821 gcatgaatct ctggaggctg aggtgaaaaa ggaatgtttt ccgccgcaaa accagacgat
    11881 aagttaaaaa cgaacaaaga agcagcaata ataagacggc gtttcaacag caaaccctca
    11941 ggagtttcaa atagctgttc tttttacgga aatacttatg aactggctgg aataaagtgc
    12001 aaaaaaatgt gactaccctc tcatttttat ctgacatgat ctgttgccac tcgctgccaa
    12061 attgtggcgc taaagctgat tagcacggtg atatttgata ctctggcaga cagcagaaat
    12121 aacggattta acctaatgat gaatgacggt aagcaacaat ctaccttttt gtttcacgat
    12181 tacgaaacct ttggcacgca ccccgcgtta gatcgccctg cacagttcgc agccattcgc
    12241 accgatagcg aattcaatct catcggcgaa cccgaagtct tttactgcaa gcctgcggat
    12301 gactatttac cccagccagg agcagtatta attaccggta ttaccccgca ggaagcacgg
    12361 gcgaaaggag aaaacgaagc cgcgtttgcc gcccgtattc actcgctttt taccgtaccg
    12421 aagacctgta ttctgggcta caacaatgtg cgtttcgacg acgaagtcac acgcaacatt
    12481 ttttatcgta atttctacga tccttacgcc tggagctggc agcatgataa ctcgcgctgg
    12541 gatttactgg atgttatgcg tgcctgttat gccctgcgcc cggaaggaat aaactggcct
    12601 gaaaatgatg acggtctacc gagctttcgc cttgagcatt taaccaaagc taatggtatt
    12661 gaacatagca acgcccacga tgcgatggct gatgtgtacg ccactattgc gatggcaaag
    12721 ctggtaaaaa cgcgtcagcc acgcctgttt gattatctct ttacccatcg caataaacac
    12781 aaactgatgg cgttgattga tgttccgcag atgaaacccc tggttcacgt ttccggaatg
    12841 tttggggcat ggcgcggcaa taccagctgg gtggcaccgc tggcgtggca tcctgaaaat
    12901 cgcaatgccg taattatggt ggatttggca ggagacattt cgccattact ggaactggat
    12961 agcgacacat tgcgcgagcg tttatatacc gcaaaaaccg atcttggcaa taacgccgcc
    13021 gttccggtta agctggtgca tatcaataaa tgtccggtgc tggcccaggc gaatacgcta
    13081 cgcccggaag atgccgaccg actgggaatt aatcgtcagc attgcctcga taacctaaaa
    13141 attctgcgtg aaaatccgca agtgcgcgaa aaagtggtgg cgatattcgc ggaagccgaa
    13201 ccgtttacgc cttcagataa cgtggatgca cagctttata acggcttttt cagtgacgcc
    13261 gatcgcgcag caatgaaaat tgtgctggaa accgagccgc gtaatttacc ggcactggat
    13321 atcacttttg ttgataaacg gattgaaaag ctgttgttca attatcgggc acgcaacttc
    13381 ccagggacgc tggattatgc cgagcagcaa cgttggctgg agcaccgccg ccaggtattc
    13441 acacccgagt ttttacaagg ctatgcagaa gagattcaga tgctggcgca gcagtatgcc
    13501 gacgataaag agaaagtggc gctgttaaaa gcgctttggc agtacgcgga agagatcgtc
    13561 tagtttgaag cctccgccgc tgttacggcg gaggacaatg ttgggtcagg ctttttgaac
    13621 ggtgatgctc cacgcagcat cgccaatctg ctgataatcg gtgatggcat gtccttcttc
    13681 tgccgcccac tgtgggattg cttcagttgc ctgggtgcaa tcaaactcaa ttaccaactc
    13741 atcgccgctg gccatttctg ccagtgctgc ttttgcttct atgagtggaa acggacagac
    13801 ctgcgtcacc acatccagct ttttaatcgc catctttaca ccttacttaa tttgccgcag
    13861 cagttgccag tcgcgcctta cgctgcggtc gaacatagac aagccaggac gcagtccaga
    13921 ctccgagaat cataaataca aggccaaccc agccctgcca ggtcatcatt gcagtcataa
    13981 ccagtccatt accgatagag caaccacctg caatactggc accgaagccc atcagtacac
    14041 cacctaaccc gctacgtaat gttgtttgtg catcagctgc gcgaacgcgg aactcacggc
    14101 tcgctttggc tgcaataaat gaccccacga agatccctaa caccaagaaa acgccccagt
    14161 taatgtattt cacgtcacct gcgaccagaa attgcagaat attggccgtt ggggaagtga
    14221 ttcctaaccc aaacatgcgt ccggttgctt cactcaaggg ccaggctaaa agcgcaatca
    14281 aaccgatgag tacagcagtg acaaagggat gccagcgttt ttcaaacaga atatgagcga
    14341 tcccggttcg gcgcggcggt aaggtcgcga ctttcagttt tggcttcttc aactctttca
    14401 tcaccaccca gagcgttatc accagcaaaa cggcaaccaa cggccacaca gacaaattaa
    14461 aagtctcagc aatagagtta tgttcagtac tgtagtgctg caaggtttga tttaaactac
    14521 tggcatgtgg agaacgcatc accgcactca tcaccatata agtgaaaagc gcgatccaac
    14581 tgccgatcaa tccctcaccc gcgcgatacc aggtcccggt ggcacatcca cccgccagaa
    14641 caattcccag cccgaagata tacccaccta taacagtacc aagccacggg aacgccccgg
    14701 cttcgtaagt cagtaggccc gcctgaatta acgcaaaaac ccccacgctt tgtaccgaaa
    14761 ttgcaatcag cagggcgtaa aacatgcgat tatttttcgc gatatacata tcgcgaaaac
    14821 cacctgtcag gcaaaaacgc ccacgttgca tgacaaatcc cagcagagca ccacaaatta
    14881 gcccgcttaa tatcattgaa aacataaatt aattaaccag atgaatgtta atgaggaaat
    14941 tattcatgac tggtggaatg cagaccaata accaaattct ctaaattaga acaaatggtt
    15001 attaatgagg atttcgataa aaaagaataa aaaaaaccgg agccaaatgt tcattcgact
    15061 ccggtttatt attagaaaag atgattatgc tacgtcttcg tactgcggaa ccgggttacg
    15121 gaagctcttg gtgacgcaag ccaggtaaat cagaccgata gcagcccaga tcagacccag
    15181 aaccattgag ctttcttcca ggttaaccca cagcgcacca acggtcagcg caccacacat
    15241 cggcaggaac agatactgga agtgatcttt cagcgtctta ttacgcttct cacggatcca
    15301 gaactgcgag atgaccgaca ggttaacgaa ggtgaacgcc accaacgcac caaagttgat
    15361 cagcgccgta gccatcacca ggtcgaagtt gattgccagc agggcaatcg cgccaaccag
    15421 gatgatgttc atcgccggag tacgccattt cgggtgtaca taaccgaaga agcttttcgg
    15481 gaatacgccg tcacgaccca ttacgtacat cagacgcgct acgcctgcgt gcgccgccat
    15541 accggacgcc agtacggtaa tggtggagaa gatcagcgcg ccaacctgga acgctttacc
    15601 cgcaacgtac agcatgattt caggctgtga cgcatccgga tctttaaagc gagagatatc
    15661 cgggaagtac agctgcagga agtaagttgc aaagatgaag atcatgccgc cgatcagcgc
    15721 ggtcaggaaa atcgcacgcg ggatcacgcg ctctgcatct ttggtttctt ccgacaggtt
    15781 gctgatgccg tcaaagccgg taaaggagaa gcacaggatt gtcgccccgg taatcatcgg
    15841 gataacatgc gcatcgccag accagaatgg acgagtgctc gccagcgtac cagcgccttc
    15901 accttcaaat acgccataaa caaccatgcc cagaatcacc gcgatcagca ctacctgcaa
    15961 cacgacgatt acagtgttga agttcgctac ggatttcaga ctacgcaggt taaaggcggt
    16021 cataaaggcc accagcgcca ccacaaacat ccacgatggg atggaaggca ccagagcttc
    16081 aaaatagatt ttcgccagca gaatgttgat catcggcgcg aacagatagt cgagcagaga
    16141 agaccaaccc accataaagc cgacagtcgg gctaatggat ttctgggcgt aagtatatgc
    16201 agagccagca gaaggatagc ggcgaaccag cttcccgtag ctcagagccg taaacaggat
    16261 cgcaatcaat gcgaacgcat aggctgtcgg cacatgacca tccgtgaggc ctgaaacgat
    16321 accaaaagta tcaaacagcg tcatcggctg catataggca agacccatca ttacaaccgg
    16381 aactaacgta agcgttttac gcaattccac gcgagaggtg tttggagtaa cgttatgcga
    16441 catggtcatt ctcctttacg gtgataaccg tcgcgtaagc aaaaaattgc cccatttttt
    16501 tggattcctc agcgacaaca actgtcgatt tttagtaaat atctatccgg tacgaagccc
    16561 ggcctcttgg tatgaatgat tggtttgaag caaaaaataa ccgacgctga tgaaacgtcg
    16621 gtttttagtc attttttgac agcggcgcat tgtgcctaaa tgggggggaa atgacaagag
    16681 aatgagaggc ttgtcataat aatttttctt taaatggctg attttccgtc atcagatttt
    16741 gcataaacac cggcgatcgc acaatttgct aaaatttcat cccgccacca gccactggca
    16801 agtcctgcgg tctgttcatt ccagccaatc cagacgggtt caaagagagt ttgggcgacc
    16861 actttcttct cgattaacgc gccactatcg agaaaacgtt gcgccagata acggggtaaa
    16921 tagccgcagc cgaggccgct aatttgcagc tccagcttgg ttttaaaatc gaaaacggta
    16981 atggcttctt gttcgtcgag aagttgtgag gccgtagaag cgcctgcctg cgcagtgtcc
    17041 cccaccacaa tcgcccgata tcgtttaatg atacgacggt ttaacggttc ttcttcctgg
    17101 gctaacggat gatgaggcgc gaccgcaaag acttgttcca gatcgcccaa ccgcgagaaa
    17161 ccaaactcac tggaagatgg tggctcatgc atcgcgccaa caataatatc cgctcgcccc
    17221 tgagtcaggg catcccagga accaccgagt acgccattga taaacttcag gcgcgtcacg
    17281 ctatgatgtt gataaaaagc ttcaatcagt ggcgcaagca gagaaaaagg aaacgtgtcg
    17341 tccacgccaa tcaccagctc gttttcccag ccttcatgca gtttgatcgc ctgcttttcc
    17401 agttcccgca cggtatgcag aacttcccgc cctttctcta ataacatttt tccggtgcgg
    17461 gtgaatttag cgcggtgacc gctacgatca agcaattgaa tattaaggtc gctttccagc
    17521 ttgtgaacgg tgtaactcag agcggacggc gtcttgtaga gtttggccga cgccgccgca
    17581 aaactgcctt ctttttctaa ggcatcgagg atcatcaaca catccagcag tggtttcata
    17641 cacgccccct tgcagtgcat gcggcgtcct gctggcgtga tcgtttactc cagcggcatt
    17701 accagcggat cgggatactg gtattcaaat cccagttcat tacaaatccg actgccatca
    17761 ataatcttac ctttgccgct gtccagacta tttctgaact gcggaggttc cagccccagt
    17821 aaacgggcca tctgcggata gaaaacatta cgcgcagggt gagctggcgc acatatatta
    17881 tagatgtgtc cgcctttagg tgcctgtaac aacagagtga tagcgccaat aacatcttct
    17941 aaatggacta aattaacacc atgttcacca tcaggcgcgg tttttccggc aaagaagcgt
    18001 ccgggatgac gccccggtcc gaccaggccc gcaagacgca gaatatcgac cgaagtaccg
    18061 ggtaaattgt gcagccagtc ttcgagttct tctaatactc gtccactgtt ggttaccgga
    18121 ttacgcggtg tagtttcttt caccgtgcct tgcgcatcgc catagacaga tgttgagctg
    18181 gtaaaaataa tgcggggaat acgatgagcc agcgcgctat ccactaactc ttgtaccgct
    18241 tgtaaataga actcatcgcc ggggccgcta cgacgtgccg gaagcgtaat gaccagcgca
    18301 tcggcatcca tcagggcatc cagatcgtca gaatcgcaaa ctaactcagg ctccatgcga
    18361 agcagatagc tatcaatgcc actcattcgg gccgcttcga caccatcttg tgtggtttta
    18421 ctcccggtga cttgccagcc tcgcgctgaa agtgacatcg ccagcggcat gcctaaccac
    18481 cctaacccga caattgcgac ctttttcatc cgttatctcc tgatacacct tagatctata
    18541 aggctacgct agcgtatcaa aactgacaat tcattctatg aatgaaatcg aaacggttag
    18601 caaatatacc attgcctgat gcgctccgct tatcagtcct acaaaaacat caatatattg
    18661 aatttaggca acatttgtag accagatatg gcattcacgc cgcatccggc aggaacaaag
    18721 cccactttag ttacagaaca caatgttctt gagggtttta atgacatatt ttcacgatta
    18781 cttttttaac ctagtcactc ttagccccct cctttccccg ctcattcatt aaacaaatcc
    18841 atcgccataa aatatataaa aaagcccttg ctttctaacg tgaaagtggt ttaggttaaa
    18901 agacatcagt tgaataaaca ttcacagaga cttttatgac acgcgttcaa tttaaacacc
    18961 accatcatca ccatcatcct gactagtctt tcaggcgatg tgtgctggaa gacattcaga
    19021 tcttccagtg gtgcatgaac gcatgagaaa gcccccggaa gatcaccttc cgggggcttt
    19081 tttattgcgc ggttgataac ggttcagaca ggtttaaaga ggaataagaa aatgacagac
    19141 aacactcgtt tacgcatagc tatgcagaaa tccggccgtt taagtgatga ctcacgcgaa
    19201 ttgctggcgc gctgtggcat taaaatcaat cttcacaccc agcgcctgat cgcgatggca
    19261 gaaaacatgc cgattgatat tctgcgcgtg cgtgacgacg acattcccgg tctggtaatg
    19321 gacggcgtgg tagatcttgg gattatcggt gaaaacgtgc tggaagaaga gctgcttaac
    19381 cgtcgcgccc agggtgaaga tccacgctac tttaccctgc gtcgtctgga tttcggcggc
    19441 tgccgtcttt cgctggcaac gccggttgat gaagcctggg acggcccgct ctccttaaac
    19501 ggtaaacgta tcgccacctc ttatcctcac ctgctcaagc gttatctcga ccagaaaggc
    19561 atctctttta aatcctgctt actgaacggt tctgttgaag tcgccccgcg tgccggactg
    19621 gcggatgcga tttgcgatct ggtttccacc ggtgccacgc tggaagctaa cggcctgcgc
    19681 gaagtcgaag ttatctaccg ctcgaaagct tgcctgatcc agcgcgatgg cgaaatggaa
    19741 gaatccaaac agcaactgat cgacaaactg ctgacccgta ttcagggtgt gatccaggcg
    19801 cgcgaatcaa aatacatcat gatgcacgca ccgaccgaac gtctggatga agtcatcgcc
    19861 ctgctgccag gtgccgaacg cccaactatt ctaccgctag cgggtgatca acagcgcgta
    19921 gcgatgcaca tggtcagtag cgaaaccctg ttctgggaaa cgatggaaaa actgaaagcg
    19981 ctgggtgcca gttcgattct ggtcctgccg attgagaaga tgatggagtg atcgccatga
    20041 gctttaacac aatcattgac tggaatagct gtactgcgga gcaacaacgc cagctgttaa
    20101 tgcgcccggc gatctccgct tctgaaagca ttacccgcac tgttaacgat attctcgata
    20161 acgtgaaagc acgcggtgat gacgccctgc gggaatacag cgcgaagttt gataaaacca
    20221 cggttaccgc gctgaaggtg tctgctgagg aaattgccgc cgccagcgaa cgcctgagcg
    20281 acgagctaaa acaggcgatg gcggtggcag taaagaatat tgaaaccttc cacactgcgc
    20341 aaaaactgcc gccggtagat gtagaaacgc agccaggcgt gcgttgccag caggtcacgc
    20401 gcccggtcgc ttcagttggg ttgtatattc ctggcggctc cgccccgctc ttctcaacgg
    20461 tattaatgct ggcgactccg gcgcgtattg cgggctgtaa aaaagtggtg ctgtgctcac
    20521 cgccgccgat tgccgatgag atcctttatg cggcgcagtt gtgcggtgtg caggacgtgt
    20581 ttaacgtcgg cggcgcacag gccattgccg cgctggcgtt tggtacggaa tctgtgccga
    20641 aagtggacaa aatcttcggg ccaggaaacg cctttgtcac cgaggcaaaa cgtcaggtga
    20701 gccagcgtct ggacggtgcg gcgatcgata tgcccgcagg cccgtcggaa gtgttggtca
    20761 ttgccgacag cggcgcaacg ccggatttcg tggcttctga tttgctttct caggctgaac
    20821 acggcccgga ctcacaggtg attttactga cgcccgctgc tgatatggcg cgtcgcgttg
    20881 ccgaagctgt cgaacgccag ttggcagaac tgccgcgtgc cgaaaccgcc cgtcaggcac
    20941 tgagcgccag ccgcctgatc gtgactaaag atttagcgca gtgcgtagag atctccaacc
    21001 agtacggccc ggagcacctg atcatccaga cccgcaacgc ccgcgatctg gtcgatggca
    21061 tcaccagcgc cggttcagta tttcttggtg actggtcacc ggaatcggca ggtgattacg
    21121 cctccggcac caaccacgtt ctgccgactt acggttacac cgccacctgt tccagcctcg
    21181 ggctggcgga tttccagaag cggatgaccg tgcaggaact gtcgaaagaa ggcttttcta
    21241 cgctggcttc aaccattgaa acactggccg ccgccgagcg cctgaccgcc cataaaaatg
    21301 ccgttacttt gcgtgttaac gcccttaagg agcaagcatg agcaccgtga ctattaccga
    21361 tttagcgcgt gaaaacgtcc gcaacctgac gccgtatcag tcggcgcgtc gtctgggcgg
    21421 taacggcgac gtctggctga acgccaacga ataccccaca gccgtggagt ttcagcttac
    21481 tcagcaaacg ctcaaccgct acccggaatg ccagccgaaa gcggtgattg aaaattacgc
    21541 gcaatatgca ggcgtaaaac cggaacaggt gctggtcagc cgtggcgcgg acgaaggtat
    21601 tgaactactg attcgcgctt tttgcgaacc gggtaaagac gccatcctct actgcccgcc
    21661 aacgtacggc atgtacagcg tcagcgctga aaccattggc gtcgagtgcc gcacagtgcc
    21721 gacgctggaa aactggcaac tggacttaca gggcatttcc gacaagctgg acggcgtaaa
    21781 agtggtctat gtttgcagcc ccaacaaccc aaccgggcaa ctgatcaatc cgcaggattt
    21841 tcgcaccctg ctggagttaa cgcgcggtaa ggcgattgtg gttgccgatg aagcctatat
    21901 cgagttttgc ccacaggcat cgctggctgg ctggctggcg gaatatccgc acctggctat
    21961 tttgcgcaca ctgtcgaaag cttttgctct ggcggggctt cgttgcggat ttacgctggc
    22021 aaacgaagaa gtcatcaacc tgctgatgaa agtgatcgcc ccctacccgc tctcgacgcc
    22081 ggttgccgac attgcagccc aggcgttaag cccgcagggg atcgtcgcca tgcgcgaacg
    22141 agtggcgcaa attattgctg aacgcgaata cctgatggcc gcactgaaag agatcccctg
    22201 cgtggagcag gttttcgact ccgaaaccaa ctacattctg gcgcgcttta aagcctccag
    22261 cgcagtgttt aaatctttgt gggatcaggg cattatctta cgtgatcaga ataaacaacc
    22321 ctctttaagc ggctgcctgc gaattaccgt cggaacccgt gaagaaagcc agcgcgtcat
    22381 tgacgcctta cgtgcggagc aagtttgatg agtcagaagt atctttttat cgatcgcgat
    22441 ggaaccctga ttagcgaacc gccgagtgat tttcaggtgg accgttttga caaactcgcc
    22501 tttgaaccgg gcgtgatccc ggaactgctg aagctgcaaa aagcgggcta caaactggtg
    22561 atgatcacta atcaggatgg tctgggaaca caaagtttcc cgcaggcgga ttttgatgga
    22621 ccgcacaacc tgatgatgca gatcttcacc tcgcaaggcg tgcagtttga tgaagtgctg
    22681 atttgtccgc acctgcccgc agatgagtgc gactgccgta aaccgaaagt aaaactggtg
    22741 gagcgttatc tcgctgagca agcgatggat cgtgccaata gttatgtgat tggcgatcgc
    22801 gcgaccgaca ttcaactggc ggaaaacatg ggcattaatg gtttacgcta cgaccgcgaa
    22861 accttgaact ggccgatgat tggtgagcaa ctcactaaac gagaccgtta tgcccatgta
    22921 gtgcgcaaca ccaaagagac gcaaattgac gtccaggtgt ggctggatcg tgaaggtggc
    22981 agcaagatta ataccggcgt tggcttcttt gatcacatgc tggatcagat cgccacccac
    23041 ggcggtttcc gcatggaaat caacgtcaaa ggcgacctct atatcgacga tcaccacacc
    23101 gtcgaagata ccggcctggc gctgggcgaa gcgttaaaaa tggctcttgg cgacaaacgc
    23161 ggtatttgcc gctttggttt tgtgctgccg atggacgaat gccttgcccg ctgcgcgctg
    23221 gatatctctg gtcgcccgca cctggaatat aaagctgagt ttacctacca gcgggttggc
    23281 gatctcagca ctgaaatgat cgagcacttc ttccgttcgc tctcttacac catgggcgtg
    23341 acgctccacc tgaaaaccaa aggtaaaaac gatcatcacc gtgtagagag cctgttcaaa
    23401 gcctttggtc gcaccctgcg ccaggccatc cgcgtggaag gcgacaccct gccctcgtcg
    23461 aaaggagtgc tgtaatgaac gtggtgatcc ttgataccgg ctgcgccaat ttgaactcgg
    23521 tgaagtcagc cattgcgcgc cacggttatg aacccaaagt cagccgtgac ccggacgtcg
    23581 tgttgctggc cgataaacta tttttacccg gcgttggcac tgcacaagca gcgatggatc
    23641 aggtgcgtga gcgcgagctg tttgatctca tcaaagcctg tacccaaccg gtgctgggca
    23701 tctgcttagg gatgcaactg ctggggcggc gcagcgaaga gagcaacggc gtcgacttgc
    23761 tgggcatcat tgacgaagac gtgccgaaaa tgaccgactt tggtctgccg ctgccgcata
    23821 tgggctggaa ccgcgtttac ccgcaggcag gcaaccgcct gtttcagggg attgaagatg
    23881 gcgcgtactt ttactttgtt cacagctacg caatgccagt caatccgtgg accatcgccc
    23941 agtgtaatta cggcgaacca ttcactgcgg cggtacaaaa agataacttc tacggcgtgc
    24001 agttccaccc ggagcgttct ggtgccgctg gcgctaagtt gctgaaaaac ttcctggaga
    24061 tgtgatgatt attccggcat tagatttaat cgacggcacc gtggtgcgtc tccatcaggg
    24121 cgattacggc aaacagcgcg attacggtaa cgacccgctg ccgcgcttac aggattacgc
    24181 cgcgcagggt gccgaagtgt tgcatctggt ggatctgacc ggggcaaaag atccggctaa
    24241 acgtcaaatc ccgctgatta aaaccctggt cgcgggcgtt aacgttccgg tgcaggttgg
    24301 cggcggcgtg cgtagcgcag aagacgtggc ggcgttactg gaagcgggcg ttgcgcgcgt
    24361 ggtggtcggc tccaccgcgg tgaaatcacc agatatggtg aaaggctggt ttgaacgttt
    24421 cggtgccgat gccttagtgc tggcgctgga tgtccgtatt gacgagcaag gcaacaagca
    24481 ggtggcagtc agcggctggc aagagaactc gggcgtttca ctggaacaac tggtggaaac
    24541 ctatctgccc gtcggcctga aacatgtgct gtgtaccgat atctcgcgcg acggcacgct
    24601 ggcaggttct aacgtctctt tatatgaaga agtgtgcgcc agatatccgc aggtggcatt
    24661 tcagtcatcc ggcggcattg gcgacattga tgatgtggct gccctgcgtg gcactggcgt
    24721 gcgcggcgta atagttggtc gggcattact ggaaggtaaa ttcaccgtga aggaggccat
    24781 cgcatgctgg caaaacgcat aattccatgt ctcgacgttc gtgatggtca ggtggtgaaa
    24841 ggcgtacagt ttcgcaacca tgaaatcatt ggcgatatcg tgccgctggc aaaacgctac
    24901 gctgaagaag gcgctgacga actggtgttc tacgatatca ccgcttccag cgatggccgt
    24961 gtggtagata aaagctgggt atctcgcgtg gcggaagtga tcgacattcc gttctgtgtg
    25021 gcgggtggga ttaaatcgct ggatgacgcc gccaaaattc tttcctttgg cgcggataaa
    25081 atttccatca actcccctgc gctggcggac ccgacgttaa ttactcgcct ggcggatcga
    25141 tttggcgtgc agtgtattgt ggtcggtatt gatacctggt acgacgctga aaccggtaaa
    25201 tatcatgtga atcaatatac cggcgatgaa ggccgcaccc gcgtcactca gtgggaaacg
    25261 ctcgactggg tacaggaagt gcaaaaacgc ggtgccggag aaatcgtcct caacatgatg
    25321 aatcaggacg gcgtgcgtaa cggttacgac ctcgaacaac tgaaaaaagt gcgtgaagtt
    25381 tgccacgtcc cgctgattgc ttccggtggc gcgggcacca tggaacactt cctcgaagcc
    25441 ttccgcgatg ccgacgttga cggcgcgctg gcagcttccg tattccacaa acaaataatc
    25501 aatattggtg aattaaaagc gtacctggca acacagggcg tggagatcag gatatgttaa
    25561 cagaacaaca acgtcgcgaa ctggactggg aaaaaaccga cggacttatg ccggtgattg
    25621 tgcaacacgc ggtatctggc gaagtgttaa tgctgggcta tatgaacccg aaagctctgg
    25681 acaaaaccat cgaaagcggc aaagtcacct tcttctcgcg cactaaacag cgactatgga
    25741 ccaaaggcga aacgtcgggc aatttcctca acgtagtaaa tattgcgccg gactgcgaca
    25801 acgacacgct actggtactg gcgaatccta tcggaccaac ctgccataaa ggcaccagca
    25861 gctgcttcgg cgaaactgct caccagtggc tgtttctgta tcaactggaa caactgctcg
    25921 ccgaacgcaa atctgccgac ccggaaacct cttacactgc caaactgtat gccagcggca
    25981 ccaaacgcat tgcgcagaaa gtgggcgaag aaggcgtgga aaccgcactg gccgcgactg
    26041 tacatgaccg ctttgagctc accaacgagg cgtctgattt gatgtatcac ctgctggtgc
    26101 tgttgcaaga tcaggatctg gatttaacga cggtgattga gaacctgcgt aaacggcatc
    26161 agtgagttgc ggggtaagcg gatgcaatat tgttgtcgca tccggcaaaa aaaacgggca
    26221 aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc gttgtaatta
    26281 cgcagagcgt tacgccccag cacaatcccc gcaccaacca tgccgcccag cagcaccgcc
    26341 agaatcaagg tgattgcctt tttcggacta tcgcgacgaa taggtaacgt cggtttcatc
    26401 acataacggt aagcatgaat atcaagatca tcaactttta agctttcgat atccagcagg
    26461 ttttgacgag tctgatagta gtttggtgag aacaccaacg gacgggtcgc ctcatgctta
    26521 atcatcgact ccagcgcttc gctccccaaa aggaacaacg tatcttgtgt cacatcttca
    26581 gtctgctgaa cctgtggctt tgtcacctgc gcctgattcg catactgcaa cgcttcctga
    26641 atctgacgga tacgcagatc tttctgctcc tgcgcgacta cttcctgagt tttcagagaa
    26701 tcctgcaagt ttttcatacg cagcgcgatg ttgtctttca ggtctttttc cagttcttcg
    26761 tttacctggt cgtcaacttg ctggatatac ttcgccagct gcttctgcgc accttcagct
    26821 gtttcaccca cataagaaac tgctaaaggt aacgactggt ttttaacggt aggctcaatg
    26881 gtcagcttct ctgcttcttc ctggttatcc agcgtttccg ctaatgctga gaatgcagtg
    26941 ctgtaacgtc cgataagcga agcctgaatg tcggagactt tcggtacagc cgatccgtag
    27001 atcacattca tcgcgttggt ataaccagcg atttgcccca catcaggttg agtaacgata
    27061 gctgtggaag tccacttttc tttggcaacg gccagatacc ctatagccag ggcaatagca
    27121 acaatgacag agatgataat tgtcatcttg ccgcgccaca actgcaccag taaatcaatc
    27181 aaatcaatct gttccgggtc atggttttgc ccagaaacat tattattttc tactctcatc
    27241 attaccctaa cggacgaaat aattttaagt cgccatagtt tagcgataat cgccatgaat
    27301 gctataggat aaatgataaa aataatgaat tattacaaag ggaacataag agaatcaggg
    27361 cgaaaatcgc cctgataaca ggatgttagt cactgccaaa gagatcgcgg gtgtagactt
    27421 tgtctgccac atccttaagc tcttctgcca tacggttgga aataatgacg tcggcttgtt
    27481 gtttgaaggt ggcgagatca cgttccaggc gagagttgaa gaatgagtct tctttcatta
    27541 ccggttcgta aatgattact tcaacgcctt tcgccttgat acgcttcata atcccctgaa
    27601 tggaagacgc acggaagtta tctgacccgc tcttcataat cagacgataa atacccacca
    27661 cttgcggttt gcgtgacaaa atggcatcgg caataaagtc tttacgcgtg cggtttgcat
    27721 cgacaattgc cgagatcagg ttattcggca cagactggta gttcgccagt aactgcttgg
    27781 tatctttcgg cagacaataa ccaccataac caaacgacgg attgttgtaa tggttgccaa
    27841 tgcgtgggtc gagacaaacg ccttcgataa tctggcgagt attcagccct aaactttctg
    27901 catagctatc cagttcatta aagtatgcta cgcgcatcgc cagataggta ttagcaaaca
    27961 gtttaatcgc ttctgcttcg gttgaatcgg tgaacagggt cgggatattt tgcttaatcg
    28021 cgccttcctg taataacgca gcaaaacgtt ctgcgcgttc cgaacgctca ccgattacaa
    28081 tacgtgacgg atgaaggtta tcgtaaagag ctttaccttc acggagaaat tccggagaga
    28141 aaataaggtt atcgataccc agacgttctt taattgattt ggtgaaacct accggaattg
    28201 tagatttaat caccattacc gcgttgggat taatttctgt cacatcgcga atgaccgctt
    28261 ccacgcttga ggtattaaaa taatttgttt tcgggtcata atcggtaggc gtggcaataa
    28321 taacgtaatc agcattttta tacgcgtcat acttatctgt cgtagcgcgg aaattgagat
    28381 ctttagtcgc cagatactct tcaatctcct tatcaacaat cggtgactgc ttcttgttaa
    28441 gcatgtcaac tttggcctga acgatatcca gtgcaaccac ttcgtggttt tgcgcaatca
    28501 gaataccatt tgaaagacca acataacctg ttcctgaaat tgttattttc atagtcgttt
    28561 agcttagcaa tattaacttg tatagttata tgtacagttg attaggaaag ttgtcaatat
    28621 aacagtacca aaaaaacctt a
//
LOCUS       DAZFKF010000062        28158 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFKF010000062 DAZFKF010000000
VERSION     DAZFKF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.062"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(34..1431)
                     /gene="ccp"
                     /locus_tag="ACHKA1_003158"
     CDS             complement(34..1431)
                     /gene="ccp"
                     /locus_tag="ACHKA1_003158"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO5680769.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1836..3485
                     /gene="treF"
                     /locus_tag="ACHKA1_003159"
     CDS             1836..3485
                     /gene="treF"
                     /locus_tag="ACHKA1_003159"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO5680770.1"
                     /translation="MLNQKIQNPNPDELMIEIDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3536..4138)
                     /gene="yhjB"
                     /locus_tag="ACHKA1_003160"
     CDS             complement(3536..4138)
                     /gene="yhjB"
                     /locus_tag="ACHKA1_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO5680771.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4658..5557
                     /gene="rcdB"
                     /locus_tag="ACHKA1_003161"
     CDS             4658..5557
                     /gene="rcdB"
                     /locus_tag="ACHKA1_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5680772.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            5606..6619
                     /gene="yhjD"
                     /locus_tag="ACHKA1_003162"
     CDS             5606..6619
                     /gene="yhjD"
                     /locus_tag="ACHKA1_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO5680773.1"
                     /translation="MTQENEIKRPTQDLEHEPIKPLDNSEKGGKVSQALETVTTTAEK
                     VQRQPIIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7012..8334
                     /gene="yhjE"
                     /locus_tag="ACHKA1_003163"
     CDS             7012..8334
                     /gene="yhjE"
                     /locus_tag="ACHKA1_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5680774.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8516..10576)
                     /gene="yhjG"
                     /locus_tag="ACHKA1_003164"
     CDS             complement(8516..10576)
                     /gene="yhjG"
                     /locus_tag="ACHKA1_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO5680775.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10646..11413)
                     /gene="pdeH"
                     /locus_tag="ACHKA1_003165"
     CDS             complement(10646..11413)
                     /gene="pdeH"
                     /locus_tag="ACHKA1_003165"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO5680776.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11645..12574
                     /gene="kdgK"
                     /locus_tag="ACHKA1_003166"
     CDS             11645..12574
                     /gene="kdgK"
                     /locus_tag="ACHKA1_003166"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO5680777.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICAELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPV"
     gene            complement(12670..14166)
                     /gene="yhjJ"
                     /locus_tag="ACHKA1_003167"
     CDS             complement(12670..14166)
                     /gene="yhjJ"
                     /locus_tag="ACHKA1_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO5680778.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(14387..15673)
                     /gene="dctA"
                     /locus_tag="ACHKA1_003168"
     CDS             complement(14387..15673)
                     /gene="dctA"
                     /locus_tag="ACHKA1_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO5680779.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15856..17844)
                     /gene="hmsP"
                     /locus_tag="ACHKA1_003169"
     CDS             complement(15856..17844)
                     /gene="hmsP"
                     /locus_tag="ACHKA1_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO5680780.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17926..21399)
                     /gene="bcsC"
                     /locus_tag="ACHKA1_003170"
     CDS             complement(17926..21399)
                     /gene="bcsC"
                     /locus_tag="ACHKA1_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO5680781.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLHQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21381..22487)
                     /gene="bcsZ"
                     /locus_tag="ACHKA1_003171"
     CDS             complement(21381..22487)
                     /gene="bcsZ"
                     /locus_tag="ACHKA1_003171"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO5680782.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22494..24833)
                     /gene="bcsB"
                     /locus_tag="ACHKA1_003172"
     CDS             complement(22494..24833)
                     /gene="bcsB"
                     /locus_tag="ACHKA1_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO5680783.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAIAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24844..27462)
                     /gene="bcsA"
                     /locus_tag="ACHKA1_003173"
     CDS             complement(24844..27462)
                     /gene="bcsA"
                     /locus_tag="ACHKA1_003173"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO5680784.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27459..>28158)
                     /gene="bcsQ"
                     /locus_tag="ACHKA1_003174"
     CDS             complement(27459..>28158)
                     /gene="bcsQ"
                     /locus_tag="ACHKA1_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO5680785.1"
                     /translation="TAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWARAM
                     LDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKSSGRYQW
                     ILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRIGSQ
                     VQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLANWCL
                     LNYSGLKTPVGSKS"
BASE COUNT         6612 a         7791 c         7113 g         6642 t
ORIGIN      
        1 gcccaaacat tcctgttcgg gcgatcaaat taattattgt ttatcctgca tatacggcgt
       61 gtaaaccccg ttcagactgt gcaggaaagc tacgatatca tccacatcct cctgcggcag
      121 ctctttgcct acctgatagc gcagcatcag tttcaccgcc ccgtccagcg tcggcacgtc
      181 accgcgatgg aagtacggtg ccgttaaagc aacgttacgt aaaccgggta ctttctgacg
      241 caatttatca cgctcttctt tagtcacatt catacgacca atatccgccg ccgtaatttc
      301 cccaaagtta aagtcttttt tcagccccaa cggttcgaag gaacgtccgc cgagaataat
      361 tccaccatga caagttgcac atttattatc tttaaataat tgatagcctt ttttctgttg
      421 cgccgtcagc gcattctcat ctccacgcaa ccatttatca aatggggaat ccggcgtaat
      481 taatgttttc tcaaattcag caatggcatc agtaatattt tcgccactga aaccttgcgg
      541 atagacttcg aggaactgcg ctttaagctg cggatctttt tccagcttag caataatttc
      601 gtcccaggat ttcgacgcca tttcaatcgg gttcaacggc ggtccaccag cctgatcctg
      661 caatgttgcc gcacgaccat cccagaactg ctcaacgtta aatactgagt taaataccgt
      721 cggcgcgtta atcggcccaa ctgcgccacc aacaccaatc gatgtttttc tgccatcgac
      781 gccccccgca ttcaacgcat ggcaatgagc gcatgaaatg gtgctatcag ccgataaacg
      841 gggatcgtga tacagcgcaa agcccaacgc cactttttgc gcatcggtag gcagtttttg
      901 cgggatgggc tgcaccggtt cattgcgatg ctccggagca gtatcattgc tggcgtaata
      961 ttccgcgcgc tgttttgcaa tccaggccag tatttccgcc cgctcttcat cactcacctt
     1021 acccgcccag tgtagcgcgg tataacgcgt tggtggcata gtttcatact gcatcaccca
     1081 ttcaatctta ttcagatcgc tttgcgaaac gggtttatca gccagcagtg ccgcacgcac
     1141 ggcctcgagg ttaaaagatt tatatccaag cttaatgtcg taatccatca actgtttcgc
     1201 gccaggaata taataatagg cgggtaattc tgccgaaggc gtgtggcaat agtcgcatcc
     1261 tttttcgcgg agaaagccta aaaccttatt attttcactg acagcagatg cctgaacatc
     1321 ggcctgttta ctgcgtttat tatcgtggta ccacacataa ccagataacc ctaaatagca
     1381 aatcgcgacg ccagccaggc cgatcgcggt aatacgtgag accattttca ttattcttcc
     1441 ctcacggtgg tgagttattg tgacccagaa aattttccag aatgctgcaa acgtctctgc
     1501 gagacaaagc catcataaaa aacagggaga tacacttttt gataggaatc aatcaattac
     1561 atgaatataa actatcaata agatagcctg tgcctattac ccagatcagg aatggcatat
     1621 catgattgcg gcgagagcag gattgaatgt tctgaaaatg aaaaacaaca ccaaatctgt
     1681 atttttataa attccatgga tattagtgcg ttatattttt tgacaggtta ataaacgtga
     1741 tccaccgcac gctttgtcgc ccaccaggcg gagcgaatga ctacccttaa agaaaagccc
     1801 gataattagc gacgaatttc ggaggttgga tccttatgct caatcagaaa attcaaaacc
     1861 ctaatccaga cgaactgatg atcgaaatcg atctctgcta tgaactggac ccgtatgaat
     1921 taaaactgga tgagatgatc gaggcagaac cggaacccga gatgattgaa gggctgcccg
     1981 cctctgatgc gctgacgcct gccgatcgct atctcgaact gttcgagcat gttcagtcgg
     2041 cgaaaatttt ccccgacagt aaaacctttc ccgactgcgc acctaaaatg gacccgctgg
     2101 atattttaat ccgctaccgt aaagtgcgcc gtcatcgtga ttttgacttg cgcaagtttg
     2161 ttgaaaacca cttctggctg ccggaggtct actccagcga gtatgtatcg gacccgcaaa
     2221 attccctgaa agagcatatc gaccagctgt ggccggtgct aacccgcgaa ccacaggatc
     2281 acattccgtg gtcttctctg ctggcgctgc cgcagtcata tattgtcccg ggcggccgtt
     2341 ttagcgaaac ctactattgg gattcctatt tcaccatgct ggggctggcg gaaagtggtc
     2401 gggaagattt gctgaaatgc atggccgata acttcgcctg gatgatcgaa aactacggtc
     2461 acatccccaa cggcaaccgc acctattatt tgagccgctc gcaaccaccg gtttttgcgc
     2521 tgatggtgga gttgtttgaa gaagatggtg tacgcggtgc acgccgctat ctcgaccacc
     2581 tgaaaatgga atatgccttc tggatggacg gtgcagaatc gttgatccct aatcaggcct
     2641 atcgccatgt tgtgcggatg ccggacggat cgctgctcaa ccgttactgg gacgatcgcg
     2701 acacgccgcg tgacgaatcc tggcttgagg acgttgaaac cgcgaaacat tctggtcgcc
     2761 cgcccaacga ggtgtaccgc gatttacgcg cgggggcggc ctccggttgg gattactctt
     2821 cccgttggct gcgtgatact ggtcgtctgg cgagcattcg taccacccag ttcatcccca
     2881 tcgatctgaa tgccttcctg tttaaactgg agagcgccat cgccaacatc tcggcgctga
     2941 aaggtgagaa agagacagaa gcactgttcc gccagaaggc cagtgcccgt cgcgatgcgg
     3001 taaaccgtta cctctgggat gatgaaaacg gcatctaccg cgattacgac tggcgacgcg
     3061 aacaactggc gctgttttcc gctgccgcca ttgtgccact ctatgtcggt atggcgaacc
     3121 atgaacaggc cgatcgtctg gcaaacgccg tgcgcagtcg gttactgaca cctggcggga
     3181 ttctggcaag cgagtacgaa accggtgaac agtgggataa acccaacggc tgggcaccgt
     3241 tacaatggat ggcgattcag ggatttaaaa tgtatggaga tgaccttctg ggtgatgaaa
     3301 tcgcgcgcag ctggctgaag acggtgaatc agttctatct ggaacagcac aaactgatcg
     3361 agaaatacca tattgccgat ggtgttcccc gcgaaggcgg cggtggcgag tatccgttgc
     3421 aggatgggtt tggctggact aacggtgtgg tacgccgttt aattggtttg tacggcgaac
     3481 cataatattt ttacagccag ccgctaactt cctgctggct gtaaaattat tctcttcagg
     3541 aggagatatt taacatcatt gccgcctggg tgcgattttt cacttccaga cggcgataca
     3601 gggattccag atgcgctttt accgttccgg tactgatatt cagcgccctg ccgatctcct
     3661 tatttgattc gcccgccgct aacatggtta aaatctcccg ctggcgggcg cttaacgatt
     3721 tgagatcttt aatgtccttt tccggcgtcg tccgccagtc tccaggcaga aacatcatcc
     3781 ccatcgccgc actatttacc gccaacgcaa atgtctcgac ggttgaatca cgaggcacaa
     3841 tggccagcac attaaaatga ataacttcct gtaaccaccg tttattgcaa tccgtcgccg
     3901 taattaacac cttaacctca ggaaattgca ccacggtttt ttgcagcaac cagtagcaaa
     3961 actcaccatc ctgatcgcca tcgagcataa ctaaggcttc agggtaactt tccagctttt
     4021 gccatagctc gtctgcctga ctggccccct gaatactcac tcctggaata cgctgctgta
     4081 aactgatttt cattccatga ataaatattg actgcctgtc aaacatgact atttgcataa
     4141 ctgaatctcc acctgaatac gttaaaaaga cttaagtagt ggaagggtat tacccgcgag
     4201 aaaaaataag aattcgccat ttggcggtgg ccattctaca gagatggcgc gtagaaaata
     4261 gttaccgata taaatagtta cagctaaacg cctgaaatta catgtcgagg gcactattta
     4321 aaacaatttt gaggatttcc ttatattaat ggttagtacg catgcaatta aaaatgaaat
     4381 tccgcgacca caagccaaaa taacaaatgg caatgagaca aaaataagca caaatagcca
     4441 atacgtcctc tattcacttt aaagggaatc gctgaaaaat acgctctgtt taaggggatt
     4501 cacctttctc agaaagctat tccgcccttt tcctgctgag aaatcgccac attcgtcatg
     4561 acaacattgt gaaacccggc attagatgtt agaaaaaaca ataaacaatg cgatatgcgc
     4621 gttactgttt cgctgtgaac aacaatcggt caaagaaatg gataaaattc acgcaatgca
     4681 gttgttcatc aaagtcgcgg agctggaaag tttttcccgc gcggcggatt tctttgcttt
     4741 gccaaaagga agtgtttcgc gccagataca ggcactggaa catcaacttg gcacccagct
     4801 tctccagcgc accacgcgac gggtcaaact cacgccagaa ggcatgacct attatcaacg
     4861 agcaaaagat gtgttgagta atctcagcga actggacggt ctgtttcaac aggatgccac
     4921 cagtatcagc ggtaaattac gcatcgacat cccgccagga atcgcgaaaa gcctgttact
     4981 gccgcgcctg tcggaatttc tctatctgca tccgggaatt gagctggaac tgagtagcca
     5041 tgaccgtccg gtagatattc ttcatgatgg ttttgattgc gtgatacgca ctggcgcgtt
     5101 accggaagat ggcgttatcg cccgtcccct cggcaaactg accatggtca actgtgccag
     5161 tccgcactat ctgacgcgct ttggttatcc gcaaagcccc gatgatctga cttcacacgc
     5221 aatagtgcgt tatacaccgc acctgggtgt acatccgtta ggttttgagg ttgccagcgt
     5281 taatggcgtc cagtggttta agtctggcgg catgttgacg gtaaacagta gcgaaaacta
     5341 tctcgccgcc ggtcttgccg gtctggggat tattcagatc ccgcgcattg ccgtgcgcga
     5401 agccctgcgt gccgggcggc ttattgaagt gttgccaggc taccgtgccg agccgctctc
     5461 cctttcgctg gtttatccgc agcgtcggga gctttcccgg cgtgtaaacc tgtttatgca
     5521 gtggctggct ggcgtaatga aagagtacct ggactgaccg actatacttt ttaagaatga
     5581 ccacgacaga aggacaaaag agcggatgac gcaggaaaac gagatcaaac gtcccaccca
     5641 ggatctggag cacgagccga ttaagccgtt ggataatagc gagaaaggcg gcaaagttag
     5701 ccaggcgctg gaaaccgtca ccaccactgc cgaaaaagtc caacgccaac cgattattgc
     5761 gcaccttatt cgcgcgacag aacgctttaa cgatcggctg ggtaaccagt ttggtgcggc
     5821 tatcacctat ttctcatttt tgtcgatgat cccgattttg atggtgtcgt ttgccgcagg
     5881 gggctttgtg ctggcctccc atccgatgtt gctacaggat atcttcgaca aaattctgca
     5941 aaacatcagc gatccgacgc tagccgctac gttgaaaaac accatcaaca ccgccgttca
     6001 gcagcgtacg actgtagggc ttgtcggcct ggcggtggcg ctttattccg gcatcaactg
     6061 gatgggtaac ctgcgtgaag cgattcgcgc ccagtcgcgc gatgtctggg aacgctcgcc
     6121 gcaagatcag gagaagttct gggtaaaata tctgcgtgat tttatttcgc tgattggttt
     6181 gttgattgcg ctgattgtga cgctttcgat cacctcggtt gccggttcgg cgcagcaaat
     6241 gattattagc gccctgcacc tgaacagcat tgagtggctg aaaccgacgt ggcggttgat
     6301 tggcctggcg atttccatct tcgccaacta tctgcttttc ttctggatct tctggcggtt
     6361 accgcgccac cgtccgcgca aaaaagccct gattcgcgga acatttctgg ctgctattgg
     6421 ttttgaagtg attaaaatcg tgatgaccta caccctacca tcgctgatga aatccccctc
     6481 tggcgcagca tttggttccg tgctggggct gatggcattt ttctacttct tcgcccgttt
     6541 gacgctgttt tgcgcggcgt ggattgccac cgccgaatat aaagacgacc cgcgaatgcc
     6601 ggggaaaaca cagccttaaa ataggccgga tgcggcgtac gaatttattc ggcctggctc
     6661 cccgtaggcc ggataagatg cgccagcatc gcatccggct ataatgcgca cataacctct
     6721 tgaaactctt ccccagagcc tcttcagcca tctatttggg agcaaacaat ttcatgccaa
     6781 ctcataaccc cagcatataa atccagttgg taacttttat ttaacctgaa accagtttta
     6841 tccaccattt ataaaattat gtgaagcatt tcatagaaga aaaatcactg gcctaaacat
     6901 tatccccttt ttgccttatt tttgaccatt tccgcgattt gttacacatt gaaatatcac
     6961 ttttgctgtg cgtaatatgg ctattcgtta gccaaaaaat aagaaaagat tatgcaagca
     7021 acagccacaa cactcgacca cgagcaagaa tacacgccga tcaactcgcg taataaagtc
     7081 cttgtcgcct ctctcattgg cacggccatt gagttcttcg acttttacat ttacgccact
     7141 gcggccgtta ttgtgtttcc gcatatcttc ttcccgcagg gcgatccaac ggcagcaacg
     7201 ctacagtcgc tcgccacctt cgccatcgcc ttcgtcgcgc gccccattgg ctctgccgta
     7261 tttggtcatt ttggcgatcg cgttgggcgt aaagcgacgc tggtcgcctc gttgctaacg
     7321 atggggattt cgaccgtggt gattggtctg ctgccgggct atgccacgat tggtattttc
     7381 gccccgctgc tgctggcgct ggctcgattt ggtcagggtc ttggcttagg cggtgaatgg
     7441 ggcggtgcgg cgctgctggc gactgaaaac gccccaccgc gcaaacgtgc tctgtatggc
     7501 tccttcccac agctgggcgc accgattggc ttcttctttg ccaacggcac tttcttgctg
     7561 ctttcctggc tactgaccga cgagcagttt atgagctggg gctggcgtgt gccatttatc
     7621 ttctcggcgg tgctggtcat tatcggcctg tatgttcgcg tgtcgctgca tgagtcgccg
     7681 gtgtttgaga aagtcgctaa agcgaaaaaa caggtgaaga tcccgctggg tacgctgctg
     7741 accaaacatg ttcgcgtaac cgtactgggt acgttcatta tgctggcaac ctatacgcta
     7801 ttttacatca tgacggtcta ctctatgacc tttagtaccg ccgccgcgcc agttgggctt
     7861 ggcctgccgc gcaacgaagt gttgtggatg ctgatgatgg cagttattgg ttttggcgtg
     7921 atggtgccag tcgccggatt actggctgat gcctttggtc gccgtaaaag catggtaatt
     7981 atcaccacgc tgatcatcct gttcgcgctg ttcgccttta acccgctgct cggttctggc
     8041 aacccgattc tggtttttgc cttcctgctg ctggggttaa gtctgatggg gctgaccttc
     8101 gggccaatgg gtgcgctgtt accagagctg tttccgacag aagtgcgtta caccggagca
     8161 tcgttctctt acaacgtagc gtcgattctc ggggcttccg ttgcgccata tatcgcagcc
     8221 tggttgcaga ctaactacgg gctaggtgcg gtggggttat atctggcggc gatggctggc
     8281 ttgacgttaa tcgccctgct gctgacccat gagacgcgac atcagtcgtt gtaataagag
     8341 tgaaaaacct gatgcgctat gcttatcagg cctgaatttc catacaatat attgaattca
     8401 caaggatttg taggccggat aaggcgttaa cgccgcatcc ggcatgaaca aagcacactt
     8461 tgccaacaat ctaaatctgt ttaattgccg gatgtcagac atccggcaat tatcatcact
     8521 tcttcatctg cgacaaaatc gtccgacact gattagcctc cccttcggaa ggtgagatca
     8581 gcgccagtaa cgccgccgcc ggtgttacca gcgttgccag tgccgcagca acagcaccac
     8641 gggcaatcaa cggcccggct ttcaccccag cctgaggatt tttaaacgtc ccacgcacat
     8701 acagcggcga acgcagtgtg ataatccgaa tccctttact ctccggatca atagtcaaat
     8761 ccagctgttc cgaagcaaaa cttgccgtgc cggtaacgtt aatcaacgcg ttctcagtat
     8821 cgaaagcaaa aatctgcggg cgtgccacgc cgttggcaat attcagattc gccgccgcgc
     8881 agttcacccg cacctcatcg tcaccaaata tcgcaccgac aatgtagttg ccgacattca
     8941 gcccaacaat ctccatcagg ttgcggctca ccagcccgtc attcatcaac agtttcaggt
     9001 tgccgttact gttgcctaaa agcgccgcca ccgagttacc gctaccgcgt agttccgcgt
     9061 caccgttcat ttcccccagc gtcttctgca tcagttccac atcgggcatc agttctttca
     9121 gtttcagtcg acgagcctga atatctgccc gcccctgcat cggcttttta tcgccttcca
     9181 gatgaatatt cgccgcaatg ctgccacccg ccatgccaaa tttcagcggt tgcaggcgca
     9241 ggtcagcatt tttgaggatg atatgagtag aaagatcgct aatcggcagg ctactgccat
     9301 gctcgatacg tcggcctttg aaacgaacat cggcatccat gacgtcccat ttatcggttt
     9361 cgaagcggtc ataaggcagc actttacccg caggctgaac gcttttttcg cccttcttct
     9421 gttcagaccg tttcgacttt tcagcccctt tcccggaatc aacgccaatc aacggtccca
     9481 ggtccgccag ccgcaattgc cgcgactcga catcaccttc cagttttggt cgtggctttc
     9541 cggtggtgta gaccagagaa ccgtggatat cgctatcgcc aatgcgccca ttaaaaccgc
     9601 gataatcaaa gaccgacgat ttttcagtgt cgattttcgc taccagccga ccatccgttt
     9661 caaacggcgg ggtatcgggc agcagaacgc ccgtcagttc atagagatca cccagtgaat
     9721 cgccagaaaa tttaagccgt aaatcgacac cgcccatctt cattgggtca ttcacgacgc
     9781 catcaaaagc aacacgggta ttacctgaac ggaaatcagc ctgtaccgga aacggcgtcc
     9841 cttcgccacg cagcgccagc ataccgccta tttttcccgt accagtgagc ggttcaccgt
     9901 tataacgtcc ctgcgccttc aggccaaaaa cgtaatcgcc caccttttct ttatccgctt
     9961 tacctttcga tccagtaact tcgctgaacg gcagtggctt gcctaacgga tcgacaaaaa
    10021 tttccagatc cgctttgctt actttgtcat caatggcgat ccgcccttga tcgaaaagaa
    10081 tattatccag ccgaaacgac catgccgacg gctttgcatt cgcgtctttg ttgtcatcgt
    10141 tggcaagatt aaacgtccag ttattgttct tttcagagag gcgaatcagg cgtgcgtcgg
    10201 gcttttccag tttgatccac ggtagccaga ccgttttggt cagcagcgcc aacggtgcca
    10261 gcgttgcctc tacgcgtggc aaatgcacca tcgtgacttc gggaatatcc ggtggattgc
    10321 caagaatgat gtcttccgca tgtacatggg gccacggcac ccagctgcgc cagccagttt
    10381 cttgtttttg ccgctcccac accacgccca gatcgccacg gatagcgaac ggacgattca
    10441 actccgcaga gactttctgg ttgatggtcg gtttgagtcg attccagtca aaagttgcaa
    10501 tcaaaatgat cgccacgaca atcaacaaca agaaagcccc tgaaatcgca gcggttattt
    10561 tgcctgcctt gctcatcgtt cgcttcctca tattcttcct gtgccagtcc taaagatagt
    10621 ccagccaggc ggaaaatgag gcagcttata gcgccagaac cgccgtattc agcgtttcta
    10681 tcggtgccgg gcgtgaaaga aaccagcctt gtgcggcgaa tgcgggcgag ttctgaacat
    10741 cacgccactc ttccggtgtt tctacgccct cgacaatcac cccgcgacaa tagcgattca
    10801 ttagatgtaa aagctgagaa aagagtgtgc ggccttccgg cgactgacgc agcatcacaa
    10861 acagttctcg cgcgattttg atgtagtcat aacgcacttc acttagtgca gagaaatttg
    10921 ccatcccggt accaaaatca tccagccaca gcgggccaaa ttcacacatc gaggcaaagg
    10981 ttgaatcttt cggcagacgg atatgctcca ccagttcgaa acgcagccag ggaagacgct
    11041 caatttggcg caggattttt ggttgctgac gcagggcgat gagcgtaggg ccatcaatat
    11101 tgaccgatgc cagcaggccg tgctctataa agaagtcggc tttttgcgcc agcaaatcaa
    11161 tctgctcttt cacaacctcc atccgatggc tgacggtgat ttcagtaaaa tagcgatccg
    11221 gcggcaggcg ttgcgaaggg ttcaagggat gcgtgaccac cgttaatagc tccacggcca
    11281 ttaaccgccc gcatgtttga tagatcggct gccaagtgta agcacgctca cactgcaacc
    11341 aaaaacgccg ttcctgcaag ctctcgatgc ttgcttcagg gttgcttatt cgctggataa
    11401 cctgccttat catcaaagat gtcctgtgtt taaaagtgat gctgcccgga ctcgacaaag
    11461 attatcggcg cgtaagggca gaactttacc gcctggccgg ggtaaatttc ctcactccac
    11521 aacggaatct ttcaggaata cgttctggct caaaaaaata atggaacact gttttaatat
    11581 ggttgaccag caaaccacca cagcgcaaac taacgctaat tttttacaga tcaggttcac
    11641 gactatgtcc aaaaagattg ccgtgattgg cgaatgcatg attgagcttt ccgagaaagg
    11701 cgcggacgtt aagcgcggtt tcggcggcga tacactgaat acttccgtct atatcgcccg
    11761 tcaggtcgat cctgcggcat taaccgttca ttacgtaacg gcgctgggaa cggacagttt
    11821 tagccagcaa atgctggacg cctggcacgg cgagaacgtt gatacttccc tgacccaacg
    11881 gatggaaaac cgtctgccgg gcctttacta cattgaaacc gacagcaccg gtgaacgtac
    11941 gttctactac tggcgtaacg aagccgctgc caaattctgg ctggagagcg agcagtctgc
    12001 ggcgatttgt gcagagctgg cgaatttcga ttatctctac ctgagcggga ttagcctggc
    12061 gatcttaagc ccgaccagcc gcgaaaagct gctttccctg ctgcgcgaat gccgcgccaa
    12121 cggcggaaaa gtgattttcg acaataacta tcgtccgcgc ctgtgggcca gcaaagaaga
    12181 gacacagcag gtgtaccaac aaatgctgga atgcacggat atcgccttcc tgacgctgga
    12241 cgatgaagac gcgctgtggg gtcaacagcc ggtggaagac gtcattgcgc gcacccataa
    12301 cgcgggcgtg aaagaagtgg tggtgaaacg cggggcggat tcttgcctgg tgtctattgc
    12361 tggcgaaggg ttagtggatg ttccagcggt gaaactgccg aaagaaaaag tgatcgatac
    12421 caccgcagct ggcgactctt tcagtgccgg ttatctggcg gtacgtttga cgggcggcag
    12481 tgcggaaaac gcagcgaaac gcgggcacct gaccgcaagt accgttattc agtatcgcgg
    12541 cgcgattatc ccgcgtgagg cgatgccagt gtaaacgcag catatgcaaa aggctggata
    12601 aagtgttacc cctctatcca gctcatccat aaatacctga tggttgtgct aactcatcac
    12661 catcaggcat tactgtgcag gtggaatatc cgtcacttca ggatgtacgt catccgtggc
    12721 gacagaggtg gtcgcagcgg cggtagatgg ggccatgatt tgatcccagg ccgcctgcaa
    12781 cgctttcatg ttaaattccg gctcgccttt cggctgcagc agtattaacg ccatatcatt
    12841 cgacaactgc tgacgcagat cctgatttaa catctccaca gtcaggctgt taaggaaatc
    12901 ctgccgcagt ttctgatact gctccggcgc gatatcgacg acctgatttt gcaacgaacg
    12961 catccgctga cccatcagaa tatcggtatc agctcgtgca taggcggcaa acagtttctg
    13021 cagctccagt ttcttttgcg ccactaacgc attgaactct tcttccggca gacctttatc
    13081 gcgaaccttc gccagttcac gcgccaccag attaaggttg ctgttcagct tgtcgtttgg
    13141 tgattcgatg ttgatggcac actgcgcacg cagatacagc acacggcagt caaatccaag
    13201 accgatgtct ttgctgttac tggcgcttaa cgcttgctga acatgccaga acagcgcctc
    13261 acgggccagg tccgcacgcc agtagcgcag cagtgcggct gattcacgaa tcggctgcca
    13321 cggcgtatcc cacatgatag ataaccggtc ctgacgcacc gcgtcagtca taatactcac
    13381 cgcttccgca cgcagcggag aaagcgtcgg caccggagcc ggtgtttcac gtttgccttt
    13441 cagttcgcca aacgttttgt tgatttggtc gacgaccgag cgcgcatcca cgtttcccac
    13501 caccagcagc gtcattgcat ccggggtgta ccatttctga tagaaatctt taattttttc
    13561 cgcttcaacg ggttgtttca gcggatcggc aggatcgtga cctaacaggg ttgatccttt
    13621 cagacgataa cgccaccagc cctctttagt atcggcaggc caggttgcca ccatgtcctg
    13681 actttgcagc gcgtggttga tggtttctgg cgtgatagtc agtttgccag tggcatttgc
    13741 cagataagag agcgcctctt tcagcaaatc gttacggtta ttgggcaaac tcagattaaa
    13801 cagcgtggtg tcataagaga caattaccgg cggcatcggg cgtttagggt cgatcccctg
    13861 ctgccacaat gaacgcgcct gtgctgcgtc aaggccaccg ctttgcgtta gcgcaatacg
    13921 agggatggcg tgactgtaac cgctctgttg tgtactttcg gcgagcgaac cggtattaac
    13981 cagcaggcga atttcaacac gatcgctggg acgctggggg gtggtcagca cttgccactg
    14041 taaaccgttg gaaagcgtcc cctgttgcca tgctggatca ggctggagcg catctgcctg
    14101 cacatagcca gcagtggcca tcatcagcaa accgcccgct aaaagtcgaa tttttgtgcc
    14161 ctgcatgtga acccctgatc aactatcctg gtaataaaaa aactgcccgt tgtcggtcag
    14221 tctaaatgac gttaaaaaca cttcgtgtta gaccgcaaga acatgaaaat gtcacggaag
    14281 aagtgaaata aacctgaact cacccaggtg gtaagttcag gcacaggggt caattatgcg
    14341 caaacacccg cactcgggga agggagtgcg ggcataagtg atgagattaa gaggataatt
    14401 cgtgcgtttt gccatccggc gcacgattat tcagcacatc gtccagtttt ttgtggtcca
    14461 gttctttcac ccacttagca acgacaatgg tcgctacgcc gttaccgacc aggttagtca
    14521 gcgcacgagc ttctgacata aagcggtcga taccgaggat cagcgccaga cccgctaccg
    14581 gcaaatggcc caccgcagag agcgtcgccg ccagcacgat aaagccacta cccgttaccc
    14641 ctgccgcccc tttagaagaa agcagcaaca cgattaacag cgtgatttgg tggacgatat
    14701 ccatctgact gttagtggcc tgggcgataa acaccgccgc cattgtcagg tatatcgatg
    14761 tgccatcaag gttaaacgag tagcctgtcg ggatgaccag ccccaccacc gatttacggc
    14821 agccgagttt ctccatcttg tcgagcatac gcggcagcgc cgactcggaa gatgaagtcc
    14881 ccagtacaat cagcagttct tcacggatgt agcggataaa tttgaagata ctgaaaccag
    14941 tcgctttagc gattgaaccc aataccagca ccacaaacag gatacaggta atgtagaaac
    15001 agataatcag ctgccccagt tgcaccagtg tgccgacgcc gtatttaccg atggtaaacg
    15061 ccattgcccc gaacgcacca ataggtgcca gacgcatgat catattgatg atgccgaaga
    15121 tgacctgcga gaaactttcg atgacgttaa aaatcagttg gcctttgctg cccagacggt
    15181 ggagcgcaaa accaaacagt acggcaaaca gcagcacctg cagaatgtta ccgctggcaa
    15241 atgcgccaat gacgctcgcc gggatgacat ccataatgaa ggcgacaatg ccctggtctt
    15301 tcgcctgatc ggcgtaaacc gctaccgctt tcgcatcaag cgttgccgga tcgacgttca
    15361 ttccggcacc aggctgcacg acgttaacga tgataagacc aataatcagc gcgatggtac
    15421 tgacaatttc aaagtaaagc agtgcgactg cgccggtacg accgaccgcc ttcatgcttt
    15481 ccatgcccgc aatgcccgtt acgacggtac aaaagatgac aggagcgatg atcatcttaa
    15541 tgagcttaac gaagccgtcg ccaagcggtt tcatttgctc gcctatttca ggatagaaat
    15601 ggccaaggag aataccaatg gctatcgctg tcaggacctg aaagtaaagg cttttaaaga
    15661 gagaggtttt catagggtgt cctttagtaa aaccacaggt cttgtaaggt tatggggtac
    15721 ctgcggcctt aaaataacac ccagacaaca tcacagaaat gtacctggat cataattgaa
    15781 acaaaaaggt taaaaagttt gagctggctc gcacaaacca gcacttttta aagttttgta
    15841 atcagtttgg ggtagctact tttcttccag gtaactctct tcgaagattt caatagggag
    15901 tggacgagca aaaaggaagc cctgggcaat accaacgccc gcttttgcca gccagtcgcg
    15961 ttgtgcttca gtctccacgc cttcggcaat catttgtaag ttcaggctct gcgccagcat
    16021 gataattgca gcaatcatgc tgctatcttc cggcaagcct tcaacaaaca ttttgtcgat
    16081 tttcagtacg tcgattggca acgatttcat atgctgcagc tgacgcagcc ctgcgtagcc
    16141 catgccgaaa tcatccagcg ccacccgaac tccggcattg cgcagcggac ggaggattgc
    16201 caccgcagca tgagggtcgt caatacgtcg gctttctgtc acttccagaa tcagtgttcc
    16261 cggctggatg cgatagcggg ttaacagttc cagcatatcc gccaccatat tcgggtgcat
    16321 cagttgcagc gcagagaggt ttaccgacaa gggcagcata atgccgcgct cttgccaggc
    16381 tgcaagcaat cgacaggact cttccagcac ccagtgaccg acggtaacca tcagcccaca
    16441 gcactcaatg cgatcgatta agccatccgg caggtcccaa ctgccatccg gttgctggat
    16501 acgcagtaac acttccgcac tgaccagttt accgctggtc atctcgacct gtggctgtaa
    16561 ccaaatagca aactgatgat tttccagtgc attaaggata tcgctctctt ccgtcaaccg
    16621 cttctgggca gcttccatct gctgcggatc aaagaactga atctgattct tgcctttatg
    16681 gcgagcggta aatgccgcag aaatagcgcg actgtaaagc tgttcggcgg tgagatcgcc
    16741 gtagaacatc gccacgccaa tgctacagtg cggacggagt tgaatacgtt caatcggcag
    16801 gcgctcgctc atgatagtga gcacttgctg acccaaagtg attgcgtgcc acggttcctg
    16861 tacaccgttg gcaatgacag caaagtcata accgctaatc tgcgcgagga tcatacgtgg
    16921 cgacagtacc gatttgagtt tttccaccag cgtcagcagc agaatttctc gttgcgcctc
    16981 tttcagcacg cccgcagtat cacgcagagt ttcacaggtg atgatcatca gcgcggtggt
    17041 ttgtttacgc gcgacaacct gctccagcat ctccatcagc aaggctttgt tcggcaaatc
    17101 cgacaccggg aagcgcatcg cattttcgtt ctgttcttca taatggcgct gcagcaattg
    17161 ctggttgagg ttgtaactgc gcaccaacat accgatttca tcgtcctgat gcagacgcgg
    17221 taatgccagt tggtgaccaa caagctcctg ggctgggatg gcgttaagtt cgcgagcaat
    17281 attgcgtaac ggatgcaaaa tcaggcggtt aatgcaccag ctaatcgcca ccgtcaatat
    17341 taacgacaaa agtaagtaaa tggtcactaa cgttgagagg gtgctcatca cgaacttata
    17401 catacggaag gaatccgcct gtagcaccag ataggcaatt ggctgcggat ttgccggacg
    17461 ttcgagcgag tagacgccca gcgagatttg aaccggtagc tcaaacaggc gagtaaccat
    17521 taccggcacc gggcgctctg gaataaaact tttacgcagc gcctggaact ggttaggcag
    17581 cactacatcg gcacggctga ccacgcctgc cggtttaatg ctggcaagaa tagcttccgc
    17641 ttcgggaata tcgcctttca aaatggctga agataagggt tcgcggacag agcgagcaat
    17701 gctttccagt tgcgtagccg tgttatagcg attctgctgg accagatgga acagcaaaac
    17761 ggtgcaaaaa ataaaaacga acaccaggac aacggctgcc accattgcca tctgcttgat
    17821 tgttaacgag cgacttacgc gcaaattgtc tccccccgga aatcccagat tgccgcccga
    17881 gtatacccga tcgcagcggc attaagagag gcgctatctg aaaacttacc agtcggcgta
    17941 gggtatcagc ggctgcggcg gtaaatccat gtcaccctgc catccagcgg cggaataacg
    18001 tacatagagc aggaaatggc tgggcgcgta atctttcgcc tgctggatat caattgccgt
    18061 accgacaaac cagttggatg tgacgcgacg ctcaagtaat gcccgcgccg tatagccaaa
    18121 gccctgactg ctgccgccat cgttggattg ccgcgcagct tcttcctgcc agtcagtcgg
    18181 gatcagattc atcagcggat accgcggcat ggttttggtg cgcgaatgcg accacgagcc
    18241 agacgccccc agctcccacg accagttttc cgtgcgctcc cgccacatca ccggtatggc
    18301 aaacgacagg tattcctgcg gactgtagta accgccctga ccgagtgagt agccactcag
    18361 gtctttgtcg taatgccaga tcatgttgtt caggccgatt gtgacgcggc gattgttctg
    18421 gttgatgacc ttatagtaat agcccgtcat ccagcgcacg cgccagttat cttcgacatt
    18481 tttaccggtt aactggtcgc cactaagcga tgcccagacg ccgtttgctt cacctttatc
    18541 gtagctcagg cttagcccca cgccgtcggc acgcacaccg ccccattttt tcccggtatt
    18601 gctcggggag tctttttgcc caccaaaggc cagcaaagaa ctggagatgg gccgacggtg
    18661 ggcgttaacg gtgtaaccca gcggcccgat atcatcgctg taactgatgc cgccgaccac
    18721 atccaccacg ttgaagccca tcggcgtggt gccgatatcc cagctccaga cgtcatttcg
    18781 ccagccgacc gccacgctgg caccggaatc cgactggctg cggttgccgc tacagtcctg
    18841 taatgtacag gtgccccagt tatcatccca tttaccatcg gcattagtgg agaaactgcc
    18901 gacgttcata ttgacgaaat cactgcgaaa gaacatccgc ccgtcagaat acggcgcgtc
    18961 cacctgcaac atggtagtgt gcgctttcag atcggagtaa ccaccggtgc cgctcgatcc
    19021 ccaataatca tgctcaaggg tgacgttaag atcctgctgg cgatagaggt ccgccgcatc
    19081 gctgcgcacg ccgcgtttca gccagtcatc tttctcgtcg ttacgggtca gtcgggtaaa
    19141 ggtgtcgtta tcctgcggac gcgtcgtagt cacaccggat gcgaccatgg cgtctttgta
    19201 ggtttccagc gcctgctttg gatcgcccgc ctgcgcttca aacttcgcac cgtcgcgcag
    19261 caccatcgcg ctttccatcg acggtggctg agattttgcc tgcgggatca acttattaaa
    19321 cgtctgctgc gccgctgcgg tatcgccaag ctgcgcctgt gccagcgcca cgcgccgctg
    19381 tgtgttcagc gaggcgttat cggtagcggg cagtttcgcc agctggctac gtgccgccgc
    19441 tttgtcaccg gcagcaatat ccacttccgt cagaccaaga atggcatcgg cgttagttgg
    19501 ctcccgcgtc aggacattct gatacgcagc gcgggcggcg gtgtaatcac gtcgttgttg
    19561 cgcccagtca gccagcgtga ggtcaatacg cgaggaaggc ggttgctggt gcagcatcgc
    19621 ttccgcttct gcctctttgc cgctttctcg caggcgatta gcggtttcca gcacctgatc
    19681 gctttgcagt cgattaacca gctcctgaat attgctgctc cactgcgcac gcggcaggct
    19741 gttgatatgc gccagcgccg ctctgtcctg gtcatgacca gagaggtaca gcccgtaagc
    19801 gtaaacctgc tccgggtcgt tcggcttctg ctgcgccaga ttgcgcatta acgtatcggc
    19861 ctggctgcgt tgtccggcct gccagagatc ctgcgaaagt cggtaagtaa tccatacgct
    19921 gccggggtcc agcgccagtc gttgccgctg aagtgctgcc gcctgcgccc atttgccctg
    19981 gttttccagt gcctctgcct gctgtgccag acggtcgttt tgcaggctgc gttcgatatc
    20041 atcaatgcta cgccgctgac tggcagagag tgaggcgata aacgcttcag ctttttctgg
    20101 cgattgctgg cggtaaatat ttgccagccc gcgcacggcg ttagtgttgc cgctgtccat
    20161 acgcaaggtc tgctgataat aacgttcggc ggcgggataa tcttttcgcg ccatcgccac
    20221 atcgcccagc cccagcactg cataactgtc ggtgttatcg acattacgcg cctgctggaa
    20281 caggcgttct gcccggtcag gattattggc tttcagcgca gcatcgccct gctggattgc
    20341 cagccagtag cggtttactt tcagcagact gttccatttg tcgttgttgc tgctgtgcgg
    20401 gtccagtgcg agggcttttt ccagattcgc cactgcattg gcgcgatcgc ctttctgtga
    20461 atatgcctgg cccagcgccc ccagagcctc actgtctttc gggttcgccc gcactgcctg
    20521 ttgtagttcg ggaatggctt tacccgccat accagagtcc accgccgcta aaccttgcgc
    20581 acgagcgcgg aaagcaggat cggccagctg tttttgctgt tctgccagtt gcgattgcgc
    20641 agccgccacg ctatcgccat cactaaagat cgaaagatat tttttcagcg cctgcacgct
    20701 ggcatcgctg acgggcatgt ctttaatctg cccgtaccag attttagagg ccccttcgcg
    20761 cccggcgttc gatttcgcca tctgttccag gacggcaaaa ccttcgtcac ggcgatcgct
    20821 actaaacagc aatagcgcca gattgttttg caggcccgta ttgcccggtg catccgcatt
    20881 gatgcgtttt aactgattaa tcgcttcgcc acggcgagcc ggaattttcg ccaccgtact
    20941 ccagtactcg acagcaatgt caccttccgg cggcgcaccg ttgaacagct tgttgtaact
    21001 cgccacagct tcttccgcat gaccggtagt cgcctgcaat cttgcctgtt gcagtgcctg
    21061 acgaccatcc ggcgtggaaa gtagcatcgt agtccgcgac gatttatacg catttgaact
    21121 cggcgctaac tgcgacagcc gatcgagctg tttttgcgcg ccatcaatat cgccctgacg
    21181 taacaaagaa cggaaacggg cggcaacgac gtccgggtta ttcggatcaa taagttccag
    21241 ccgatacaac gactgctgca ccaggtcttc acgatgggtc gcttcgccta accgaacttg
    21301 ctccagcaac tgttgctgag cggttggtgc tgcctcgacc atcggcatga cggccagacc
    21361 gagggaaagc gtgaatatgt ttagtgtgaa tttgcgcatt cctggcccca gtcaggtaat
    21421 aactcacctt ttgtcgagaa gcggaaacgg tgttgatccc agccttgtcc aaacagggtc
    21481 agcacatagt tgtaataggc atcgctgccg ggaaagttat cggccacgcg ctggcgctga
    21541 acggcctgcg catcacggtt ttgtaaaaag ggcagcatgg cggcagaaaa accgaccggt
    21601 cctttaccct gcgctttccc cgtagccaca tccacttttt ccggcggata accgtttttc
    21661 tcagtgaatg tcgccatcgg tttaaaccgg ttgagcatcc gcgctttttg cggatcgctg
    21721 tcaggcatca tgcctaccca catgtaaacg cggatggcgt cgtagctgct gatcaaggtt
    21781 ttttcggctt ttagctgcca gcctttgtct ttctcatagc gcacccagtc tggcgaaaag
    21841 cctttcgggg cggtttccag caataaacgt tgattggttt cgcgcagcgt ggtccacggc
    21901 gcgccaaagc gggtgaaata ctgcgccagc gtcggcggca ggtagctggg gttaaaacgc
    21961 cagctgttat cctctgcaaa acccactttg cccggtaaca acatggaacc cagcccaggc
    22021 accgtcacca cttcctcccg cgcaatacgt tttagcaacg cgctgccgat gtcggtataa
    22081 cgctgctctt tccacaaacg ccccgcctcc agcaacgacc aggccatcca gacatcacca
    22141 tcggaggccg aattgctgtc cagcacttcc cacttactgt tctctttctt gccccacagc
    22201 caggcgggca aacgttcttt taaagaaccc tgagcgagat tgttctgcgt ccagtcgaga
    22261 atattatcga aagctgcacg gtcgttagcc gccagggcaa agaacatgcc gtaactttgc
    22321 ccttcggagg tggtgatttt gcgcgcgtcg ctggggtcga tgacgcgccc ttcctgactg
    22381 atgtaatcct ttttaaactg ctcccaggca ggccaggtac aggctgcctg aacactaaag
    22441 gcagccaaca gcagcatcgt cacgagtcca ctacgcaaca cattcatctt caattactcg
    22501 ttatccgggt taagacgacg acgactaata atacgcagca gacgccacag tacccatgcc
    22561 agcaatatca cactgatagc cgccagcacc gccagcagaa tcggatggtt tgccagcgca
    22621 taccacaagc gctcgaacca cggcagatga cctacgtaat aaacgtcgcc aacacgtagg
    22681 ctgttgatac cggactcgcg gatcaccgcg accgaaccga acatggtggc gcgtttgccg
    22741 ctatcgttca ccgcatcgtt aagcatttca taaccgcgtg ggctatctgc cagcagcgca
    22801 atcacgctgc gctggtcgtt atacggcgac tggaagccaa tcaccgccgc catcgcaccg
    22861 gaagaggtca gcgttgactg ggtttctgcc gcgcgatcgc tctcgtccgg cacaatgccg
    22921 gggaacgggg tctggcgcat cggcgttttc acccagcttt cggtcgcctg caccaacagg
    22981 tcgatctgct tatcgtcttt cagtttgtcc gggataccac cgatgatcat gatgtcggca
    23041 tctttgccct gaatggtgct gccatcatcg gtcaccgtca gattaatcgc cgggaagccc
    23101 gtctgtgcgc cgataaaacc aacagtattc agcaacgttt ccatctgtgc ttcgttaggc
    23161 gttttcggca tcacggtgat ggtttgcgac agatccgcca tccggctgaa cgggaagccc
    23221 gcgttagcaa aggcgcgtag atccggcatc gggatgaagt ggtaatactt cgagaagtcg
    23281 atggtggaat cgtcaccaat caccacatga ttctgcaccg gctggaaggt aatacagtta
    23341 tccaccgaac cgcccggcat cgggttcata tactcaaagt cgaagcgcag ctggttggtc
    23401 gcgcccagtt tcagcgccgg aatagagaca tccgttttgc catccagcaa accttgtaat
    23461 accggaatcc gaagcagcag gcggttcgcc tcctgtttac tgctcaggtt gaaggattgc
    23521 aggaactggt tattcaggct gatatccatc cgcgaactgt ctttcaccgg cggcatggtg
    23581 tagcggtaat taatatccat atcaatgccg gtactgcgca tcaggtagag atccggcggc
    23641 aggtttagcg aaacgttaat cgctgctggc tcaagaccgc tggattgtaa ctgttcttca
    23701 taggttttca gttcgccaaa tgtgaccgga cgatcggtac gtacccagtt cggcgcatcg
    23761 tacggcttac gcggtagcag cggtttcact tcattcacta ccacgctttc accacggaac
    23821 agaatgttac cctgagcgat acctttcgct gcctgcaaca ggtctttgtc gtcacgacca
    23881 aacaccacca gcagtttgac gtaaggattc tgcggatggt taatcatctc aatcaccggg
    23941 gcttttaccg ccggatgatc gcgcaggaag tccggccgtt tgtcgttggt tgcaaagaca
    24001 atggcattgc gatccggcag ttggttatag agcaccggga agttctgccc acgccagcca
    24061 gaacgcgaac caaaccacga ggcgacaatg gcagatgctt gttgcagccc aacatccggc
    24121 gcacccgcaa agaccatcgg caaggtgttg gtgcggttat cacgcgggtc aaagaatggt
    24181 accgggaagt gtgacaggtc attcttcaca ttcagggtct gataggtcaa atccagtcca
    24241 ctgctccgcc caacatccag ccaaagcgtg gtgctggccg ggttttcgca cacgtcctga
    24301 taatggccga caaactccag ccgcacacgg ttgaagtcgg taataaacag tgggttaatg
    24361 ggcatttgcg ccagcgtttt tttacccaac tgttctttgg tcactggcag cacgcccatc
    24421 agctcatcat tgagataaac ctttaactgc gactggacag gcagtaacga tggcgatggc
    24481 gtgtattcga ggttgagcat cgccttcgtc accacttcat cgctgcgcat accaaactca
    24541 atgctgccgt tcgggttaat gccacgtagc accatgctgc ccggcggcgg cgcaatttgc
    24601 gcaaaggtca gcttcacatc acgcgaagga ccgttctgcg ccacgactgg cgcatcagcg
    24661 ccctgcacgc ccggcatcac ttgccccacc tgcggatttt gttccgtctg ggcggctata
    24721 gctggctcag cattgatcag tggttgcgtt gctggcgtcg cctgcgtcat gaaagagggg
    24781 aacgcactca tccccatagc cactgcacaa atccagaata gttttctttt catcgcgtta
    24841 tcatcattgt tgagccaaag cctgatccga tggttgtgcc gtttcgctcc gctccgggcg
    24901 gcgcggaata aacgatacaa cccaggaaac cagagaagtc agcacacgga atatgccctt
    24961 caccgaagaa ggcgcaaact ccgccagatg gcggtagcca cggaagccga gcttcagaat
    25021 atccagcaga ctttccagcg gcttatcttc cggatagctg tcctgccaga gcgcccatgt
    25081 atccgcacgg gcaaacgtac actgcacaaa atcgatatgt tgctgggtgg tgagcggcat
    25141 taatttcagc ccaacttcat tacccatcac gcgcgccacc tgggtcggga agacgtattc
    25201 ctgctgaccg cgtttaagca acagattcac tttctgccct tccagaatct gcgcctgacc
    25261 gttgatcttg atccccaaac caccgtcgga gaaatcctga acggtacacg agaagaggtg
    25321 accatcttcg cgggcaattg ccgcgggcat cgtcatctcc acgcggtgcg atcggcgtac
    25381 ctgtttgctt tctaccgata ccgcaactgc gccgccaaga acaatcaggt tgtagaatac
    25441 ccacaccata ctgacgacca cggtgagcat ctcggttggc gggccataga agtagcgcca
    25501 gatgcctacc gcaacgccca ccaggttgag cagaacaagg aagatgtagg gccgcgagat
    25561 cacccagtcg acgtactctt cttccaccag tccacctttg gcggtgacgt taaatttgcc
    25621 tttgtgcggg ttaatcagcg ccaccagcgt cggtggtgcg atataccacg ccagcaccgt
    25681 ttcgtagatt tcactccaga aagagtggcg atatttgccc tggatcttgg agttggtcag
    25741 gctggcatgg atcatatgcg gcagcacgaa cagggcgatc atcaacgctg gcgcatagat
    25801 gatgtaggca tgaagcagca ggaacgccag cggcgcagtc aggaagatca gccgtggaat
    25861 gcccgacaag aagtggaaca tggcgttgac gtaacatagc cgctgagcaa acttcagccc
    25921 tttaccggtg agcgggttat cgagacggaa gatttgcacc atcccgcgcg cccagcgaat
    25981 acgctgaccg atatgcgccg acagactttc ggtcgccagc cccgccgcct gcggaatacg
    26041 catatatgca gaggtgtagc cacgacggtg caggcgcaaa gaggtatgcg catcttcagt
    26101 cacggtttcg acagcaatgc cgccaatttc atccagcggc ttacggcgaa tcaccgcaca
    26161 ggaaccgcag aagaaagtgg cgtcccacat atcgttgcca tcctgcacca ggccatagaa
    26221 cagcgtgcct tcgttcggtg ttttacgaaa acgccccagg ttgcgttcaa acgggtccgg
    26281 cgagaagaaa tggtgtgggg tttgcatcat cgccagctgt ttctctttca ggaaccagcc
    26341 catggtcatt tgcaggaacg atcgcgttgg tacgtggtcg cagtcgaaaa tcgacacgaa
    26401 ctcgcctttg gcatatttca gcgcattgtt gatgttgccc gctttcgcat gttcatgagt
    26461 ggtgcgggcg atatatttca cccccacgtt ttgcgcaaac tggcgaaact cttccctgcc
    26521 gccgtcatcg aggatccaga tgttcagttt gtctttcggc cagtcgatac ccagcgaggc
    26581 gtaaatggta tttttcacca cgttgagatc ttcgttgtaa gtcgggacaa agatatccac
    26641 cgacggccac agcgacatat ctttcggcaa tggcaccggc tgacgattca gcggccatac
    26701 tacctggaag tagccgagca ccagcacaat ccacgcgtac gtttcggcga agagcagaat
    26761 aagcccgcac accaggctga ccggatcgtc ccagttcagc gtagaggtgt aacgccacca
    26821 gatataacgg caagaaacag tcagcgacag cacaatcaac attagcgctg agaagcgccc
    26881 cggcatccgc cgtacgatca gcgctacccc ccacagcagc atcaggaaga taaactgcgc
    26941 cagcgggtta aacggctgag taacgcagat taacgccaga atcagcgaga aggtgacgat
    27001 gataccgagg atcaaccgcc gcgccccggc actcaaatga ccgagctctt ttttctcatc
    27061 aagatgctgt gttttatggc taacgcgctc aggcagctcg ttcatccatt gatggtaacg
    27121 tccacgaata ttctgcagac ctgaaaatgc ccgcctgcgc ggtttcggcg tttctttgcg
    27181 cgatgtaccg atcagtaacc agcatgtttg aatgagataa cggaccgggt ccagcggacg
    27241 cggacgcgag gcgttgatat gcggatacag gtttttatgt tctgcgcgaa tacgctgcca
    27301 gcgcgggtgc tccagcggaa taaaaatcca ggccaggatc atccagaaac agccgagcgt
    27361 cgcgctgaaa gccgacgcac cgtgacgacg ataatcgcga taacgcccga taagccgcgc
    27421 gttgaccggc gggattagca accaccgggt caggatactc atgatttact cccgactggc
    27481 gttttcagcc cggaatagtt caacaggcac cagttcgcca gcgtcagtat ctcttcagcg
    27541 gccagcgcat cactgcgata ttcacctact ggttgcttag ccgccaggca ttcagccatc
    27601 gcttcatcac gatgaatgag catcggcagt aatcggcgct ggctttgcaa ccaaagctgg
    27661 taaatatcgt cctgaacctg actgccaata cggaagtcat taatcaaaat atgtgcgcca
    27721 tccggcagcg cttgctgatg cagtcggata tggcagttgg catccacatt gacgattgcc
    27781 agcgagtgat cgcacaaact cagcagctgg tgggttatct gcgaggcatc acgcggtaag
    27841 tcgattaaaa tccactggta acgcccgctg gattttagtt gctgtaagcc ggagcaaata
    27901 tcgctcagcc gagtttgcca gtgctgtgga ttttcttgtt cttcaatgga taactgacca
    27961 aaaggcagca aatcgagctg cgaggtgtag cgcaacccag cgtcacgcca gtcctggcca
    28021 tccagcatag ctctggccca gccctgacgg tgggtaaaat caacgttaaa tgacaggcgc
    28081 aacaagttgt ccgggcaggc atcgaccacc aggacatttt ctcccaacat ttgtaatgac
    28141 caggctaatg cggcggtg
//
LOCUS       DAZFKF010000063        27996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFKF010000063 DAZFKF010000000
VERSION     DAZFKF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.063"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            32..694
                     /gene="acrS"
                     /locus_tag="ACHKA1_003175"
     CDS             32..694
                     /gene="acrS"
                     /locus_tag="ACHKA1_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO5680786.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(697..876)
                     /locus_tag="ACHKA1_003176"
     CDS             complement(697..876)
                     /locus_tag="ACHKA1_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO5680787.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(960..1844)
                     /gene="yhdJ"
                     /locus_tag="ACHKA1_003177"
     CDS             complement(960..1844)
                     /gene="yhdJ"
                     /locus_tag="ACHKA1_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO5680788.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLSTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1930..2226)
                     /gene="fis"
                     /locus_tag="ACHKA1_003178"
     CDS             complement(1930..2226)
                     /gene="fis"
                     /locus_tag="ACHKA1_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO5680789.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2252..3217)
                     /gene="dusB"
                     /locus_tag="ACHKA1_003179"
     CDS             complement(2252..3217)
                     /gene="dusB"
                     /locus_tag="ACHKA1_003179"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO5680790.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3546..4427)
                     /gene="prmA"
                     /locus_tag="ACHKA1_003180"
     CDS             complement(3546..4427)
                     /gene="prmA"
                     /locus_tag="ACHKA1_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO5680791.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMTDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4439..5890)
                     /gene="panF"
                     /locus_tag="ACHKA1_003181"
     CDS             complement(4439..5890)
                     /gene="panF"
                     /locus_tag="ACHKA1_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO5680792.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFIGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5880..6122)
                     /locus_tag="ACHKA1_003182"
     CDS             complement(5880..6122)
                     /locus_tag="ACHKA1_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO5680793.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLAAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6231..7580)
                     /gene="accC"
                     /locus_tag="ACHKA1_003183"
     CDS             complement(6231..7580)
                     /gene="accC"
                     /locus_tag="ACHKA1_003183"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO5680794.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7591..8061)
                     /gene="accB"
                     /locus_tag="ACHKA1_003184"
     CDS             complement(7591..8061)
                     /gene="accB"
                     /locus_tag="ACHKA1_003184"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO5680795.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9039..10013)
                     /locus_tag="ACHKA1_003185"
     CDS             complement(9039..10013)
                     /locus_tag="ACHKA1_003185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO5680796.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10165..12105
                     /gene="csrD"
                     /locus_tag="ACHKA1_003186"
     CDS             10165..12105
                     /gene="csrD"
                     /locus_tag="ACHKA1_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO5680797.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPTTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12410..13453
                     /gene="mreB"
                     /locus_tag="ACHKA1_003187"
     CDS             12410..13453
                     /gene="mreB"
                     /locus_tag="ACHKA1_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO5680798.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13519..14622
                     /gene="mreC"
                     /locus_tag="ACHKA1_003188"
     CDS             13519..14622
                     /gene="mreC"
                     /locus_tag="ACHKA1_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO5680799.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            14622..15110
                     /gene="mreD"
                     /locus_tag="ACHKA1_003189"
     CDS             14622..15110
                     /gene="mreD"
                     /locus_tag="ACHKA1_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO5680800.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15119..15712
                     /gene="yhdE"
                     /locus_tag="ACHKA1_003190"
     CDS             15119..15712
                     /gene="yhdE"
                     /locus_tag="ACHKA1_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO5680801.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15702..17171
                     /gene="rng"
                     /locus_tag="ACHKA1_003191"
     CDS             15702..17171
                     /gene="rng"
                     /locus_tag="ACHKA1_003191"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO5680802.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17239..21039
                     /gene="yhdP"
                     /locus_tag="ACHKA1_003192"
     CDS             17239..21039
                     /gene="yhdP"
                     /locus_tag="ACHKA1_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO5680803.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGDV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21378..22823
                     /gene="tldD"
                     /locus_tag="ACHKA1_003193"
     CDS             21378..22823
                     /gene="tldD"
                     /locus_tag="ACHKA1_003193"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO5680804.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGAMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22951..23880)
                     /gene="aaeR"
                     /locus_tag="ACHKA1_003194"
     CDS             complement(22951..23880)
                     /gene="aaeR"
                     /locus_tag="ACHKA1_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO5680805.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            24063..24266
                     /gene="aaeX"
                     /locus_tag="ACHKA1_003195"
     CDS             24063..24266
                     /gene="aaeX"
                     /locus_tag="ACHKA1_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO5680806.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24274..25206
                     /gene="aaeA"
                     /locus_tag="ACHKA1_003196"
     CDS             24274..25206
                     /gene="aaeA"
                     /locus_tag="ACHKA1_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO5680807.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25212..27179
                     /gene="aaeB"
                     /locus_tag="ACHKA1_003197"
     CDS             25212..27179
                     /gene="aaeB"
                     /locus_tag="ACHKA1_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO5680808.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPINEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27271..27543
                     /locus_tag="ACHKA1_003198"
     CDS             27271..27543
                     /locus_tag="ACHKA1_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO5680809.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27599..27862)
                     /gene="yhcN"
                     /locus_tag="ACHKA1_003199"
     CDS             complement(27599..27862)
                     /gene="yhcN"
                     /locus_tag="ACHKA1_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO5680810.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6719 a         7016 c         7451 g         6810 t
ORIGIN      
        1 tcgaatatat ttatttcctg aataattaat catggcaaaa agaaccaaag ccgaagctct
       61 gaagacccgg caagaactga ttgaaactgc catcgcccag tttgcgcagc atggcgtaag
      121 caagacgacg ctcaacgaca ttgccgacgc cgctaacgtt acgcgtggcg ctatctactg
      181 gcacttcgaa aacaagactc aactgtttaa tgagatgtgg ttgcaacagc cttcattgcg
      241 ggagttaatc caggaacact tgacggctgg attagagcat gacccgtttc aacaattgcg
      301 tgaaaaattg attgtcggct tgcaatatat tgccaaaatt ccccgccagc aggcgttgct
      361 gaaaatctta tatcacaaat gtgaatttaa tgatgagatg ctggccgagg gagtgatacg
      421 cgaaaagatg ggctttaatc cgcagactct ccgcgaagta ttgcaggcgt gtcagcaaca
      481 aggttgtgta gcaaataacc tcgatttaga tgttgtgatg attattattg atggtgcctt
      541 cagcggaatt gttcaaaact ggttaatgaa tatggcgggt tatgatcttt ataaacaagc
      601 ccccgctctg gtcgataacg tattaagaat gttcatgcca gatgaaaaca taacgaaatt
      661 aattcatcaa acgaatgaat taagtgtcat gtaaattcac attctgtcct gaaaattcag
      721 ttcgtcacag ttaaccagtt ttagtggatt aactgagttc atattgcggc atttgtctgt
      781 ttctgttgcc agcagttcaa tccactgcat cagtaaagta tcaaacagaa aaacgaagac
      841 agcgaaaacg acgagccacc aatacttgcg aatcattacc tgagcctgtg catgtttaac
      901 caagtctggc aggaaatata cacgaaatga ataatctgaa aaccaggctt acacttcctt
      961 tactttgcaa tgagatcggg gtcaacttcg cttaaccgac ttcgttttga caggttgccg
     1021 gtctttcttt ttttcacttt cgccagttct tccgcagagt aatgcgacgc gacatccagc
     1081 cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa taccaatgaa ttttcgcccg
     1141 ctggagacgg ctacggcacc ggtagtaaag ctaccggcaa acgggtcgag aacgatatcg
     1201 cctgggttgg aagaggcaag aataatgcgt ttcagtaagg cttccggttt ttgcgtcggg
     1261 tggttctcat attcatccat caaataacgc acgcgcggaa aatcccagac gttacccggt
     1321 actttttgat gattgtaagg ctgtggagga tttttgcgat aatcgatcaa cgcgcgctgc
     1381 gatccagttt tggcttctac cagaatagca tcaccgttga acgtgtagtt ctttgcgtct
     1441 ttcaccatca tcaggatggg ttcgtacatg gaaccgtagt gttttttcgc ctgcactcca
     1501 gaactgtcat atgaccagac gatgcgactt ttgatggtaa aaagcttgcg gcactggaga
     1561 tcgataaagg gcatgttttc cgtgctgttc atgatgtaca tgctgccctg ctttttcaga
     1621 acgcggtggc actctgcaat cacttcaaac agccagtcga taaacagatc ttctttccag
     1681 gcttcgatca gaccatcaaa atttttaccg atgttatacg gtgggtcggc aaagatcaga
     1741 tcgacgcttt cagtagatag ctttttaagt tcggcaaggg catcaccgtg aataatggtc
     1801 ttagcttcat taccaaaccg ggtcggttca catcctgttc tcatggtcac tccctttgtg
     1861 acacctataa aaaaggcgct tccccatgcc gagtagcgcc tttttaatca agcatttagc
     1921 taacctgaat tagttcatgc cgtatttttt caatttttta cgcagcgtac cacggttgat
     1981 gcccatcatc agtgcagcac gggtctggtt accacgggtg tattgcatca ccatgtccaa
     2041 caggggctgt tctacttcag ccagtaccag ctcatagagg tcattcacat cctgaccatt
     2101 cagttgagca aaatagttct tcagtgcctg tttaaccgag tcacgcaggg gtttttgggt
     2161 tacctgatcc tgagagttaa cggtagaaac ggtcagtacg tcagaattta cgcgttgttc
     2221 gaacatagtt ctgtcagctc tttatttctg tttacgcaaa attttcgaag tatgcctcca
     2281 acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa tgtgcgccga aactggtcat
     2341 ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg tgcaattcgg tacccttttg
     2401 ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca aagcaagcgc ttaacctctg
     2461 ccaaaggcag cgggggcagc aactccccag tgtccagata atgctggatt tcccgaaaga
     2521 tccagggtct tccctgagct gcgcggccta tcatcagggc atccgcccct gtatagtcga
     2581 gcacagctct ggctttaagc gggtcagtaa tgtcgccatt cgcgataacc ggaatggaaa
     2641 ctttctgctt aactgcccga atactgtcgt actcagcttc tccattgaac aaacaggcgc
     2701 gtgtacggcc atgaatggtc agagcctgaa tgccacagtc ttcagccagt tgggcaatct
     2761 cttcgcagtt acggtgttcc ggcgcccagc cggtgcgaat cttcagggta acaggaacgt
     2821 ccactgcatt gacgacctcg gtaaggatcg atttaacgac atccgggtac tgcaagaggg
     2881 ctgaacctgc gagcttgcga ttcacttttt tagccgggca acccatattg atatcaataa
     2941 tctgggcacc gctttccacg ttaatacgtg ctgcatctgc catttctttc ggatcgctac
     3001 cagcaatttg cacggtgcga ataccgggtt catcaatgtg caccatccgt aaacgagatt
     3061 tgtcgctttc ccaaacctgt gggttagaag acatcatctc ggatactgtc aatccggctc
     3121 ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat gccagccatg ggcgctgcga
     3181 tcaggcgatt tctgagctga tattgtccga tgcgcatgag ttaagaaatg accatactgt
     3241 gactgcaagg cggcgtatat tacgcatttt ttgcacgaga tgaaaggcca aactttgacc
     3301 aatcctctga gatggatcaa agaattgcat ttaaaatgag cgtggtgcga taattactca
     3361 taaaaatcat catattagaa aatagtgact aaaatttaca ctcaaagaaa tttgagtaag
     3421 ttctcaattt ttctttatga atgaaaattt tggcacgcaa atttgcgtaa ataatcggca
     3481 aatttacgtg ccttttgtga gcttgctcgc acttcgcccc gcgtcaccct acggcgatgc
     3541 gaaggttaat tcttacgacc ggtaatacgg caccactctt ctttttccac gaccgggtcc
     3601 agtgcgaagc tgtcggcata agcttcacaa acgctctctg cctggcttgc cagaataccg
     3661 gaaaggccca gcaaaccgcc tgaaaccggc aggacgctga ttaacggtgc cagttcacgt
     3721 aatgggcctg caaggatgtt agcgaccacc acgtcggctt tcatttcttc tggctgatct
     3781 ttcggtaagt agagttccag acggtcagaa acgccattac gttcggcgtt atcgcggctg
     3841 gcctgaatcg cctgcggatc gatatcaata ccaatggctt ttgctgcacc cagtttcagc
     3901 gccgcgatcg ccagaatacc ggaaccacag ccaaagtcga tgactgtttt accggttaaa
     3961 tcgaggctgt cgagccattg caggcacaga gaggtggttg gatgggtacc cgtaccaaac
     4021 gccagccctg gatctaacat cacgttgacg gcgttttcgt ccggcacatc acgccagctt
     4081 gggcagatcc acagtcgttc accaaagcgc atcgggtgga aattatccat ccattcgcgc
     4141 tcccagtctt tatcttccag ttgttcgatt ttatgcgcga agcctgcgcc gagcagcgga
     4201 tggttttcca gaatcgccac cacgtcggtc atatcggttt cagcgtcgaa cagaccaatc
     4261 acatcggtgt cgccccacag gcgcgtttcg cccggcagcg gctcaaatac tggcgtatcg
     4321 tgggtgtcct gaaaagtgat agaaacggca cccgcttcca tcagcgcatc gctaagatct
     4381 tccgcgttcg cgccggtggt gttcagtttc agttggatcc aaggcatggc aaaactcttt
     4441 atttatcagt agtcaaaacg gtagcttgcg ggacggatgt accgaaacgg tttccgacca
     4501 ggaaagccag caaacttagt agtaacgagg gcacgatagg gtggaagccc aggtactgaa
     4561 tattcagcgt cgcgagtacg gcatacagca cgccgccaac gatcatcgca cttagcgcgc
     4621 ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag cggccacagg aaaacggctt
     4681 ccagcccacc gaaggccagc aaattcagcc agatgatcat ttctggcggc ttccaggcgg
     4741 caagcagcag caacgcgccg agaactaacg taattaccgc cgacatccgc ttcagacgcg
     4801 tctcgttttg catttgatcc ggacggatat tcagatagag atctttaatg atcgtagcgg
     4861 aactttgtag caattgggcg ttaattgtcg acatgatcgc agccataggt gcagccagaa
     4921 agatcccggc agcaaacggt ggcagcactt ttaccattaa cgtcgggatc accagatccg
     4981 gtacggtgag atcggggatc actgcacgac ctaacgctcc ggccaggtgc ataccgaaca
     5041 tcaggattgc gactacaatc gtaccgatga tgatcccccg gtgtactgct ttgctgtctt
     5101 tataagagat acagcgcacc gcagtatgcg gcaggcctat aacgccaaag cacaccagta
     5161 cccagaacga cgtcataaag gcaggcgaca gaatatcgtc agcgccttgt ggcgtaacca
     5221 gttgcggatc gatggtttgc aaggtctgta ctgcgttgct taagccgcca gcggcatgta
     5281 ccacgccaat aagcagcaca acggtgccaa tcagcatcac aagcccttgc atggtgtcgt
     5341 tcagcacgct ggcgcgaaag ccaccaaatg cggtatataa cgcaatgctg ataccaaaaa
     5401 tcagcagccc ggtttcataa ggaatacccg ccgcggtttc cagcaggcgc gcgccgccga
     5461 taaactgcac ggtcatcgcg ccaataaacg cgaccagcaa actcaaactc gccagccaca
     5521 ccaaaagacg actctgatag cgggcaaaca gcatatcgtt cagcgtcact gcattgtagc
     5581 ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag tgaaagccag actgcaggaa
     5641 gctgaatcat cgccagcaat acccagccaa ggccgtattt gtaagcagct ccaggcccgc
     5701 cgataaacga actggcactg atataagtcg cggtgagcgt catcgccagc acaataccgc
     5761 ccatagagcg gctgccgagg aaatactcat taaggaaggt gccggtgctc cgtttacgca
     5821 tcgcataaac cgagataccg aacaccacca ccagataggc gaccagcggt agaattactt
     5881 caagctgcat cgtcatcctc cagcgggata tcgcgataga taaatttcac catcgcccag
     5941 cacagtccaa taaacagcag cggcgtcagg atgcaggcca tctcaaacca gcgcggaaag
     6001 ccggtaaaac cgggggcaac gccaggtaag taagcggctg ctaaccaaac tgccagatac
     6061 aaaagggtca gccccagcgc ccagcgcgcc tctttatggg cctgaacaaa acgagtgtcc
     6121 attttttgtc cctgatgggt gaagaaagcg aggattgtac cttatggggg ttgtcgatcc
     6181 ccagtaataa aaaaggccgg aaaatccggc cttttgacgc ttttgcagtc ttatttttcc
     6241 tgaagaccga gttttttctc cagatagtgg atgttagtgc caccatgctg gaagttctcg
     6301 tcattcatga tgcggatctg cagatcaacg ttggttttga taccgtcgat gatcagctcc
     6361 tgcagcgcat ttttcatgcg ggcaatcgcc acgtcacggt tttcaccgta gcaaatcagc
     6421 ttaccgatca ttgagtcata gtacggcggt acggtgtagc ccgcgtagat atgagactcc
     6481 caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga ttttgcccgg acttggcagg
     6541 aaggtgttcg gatcttcggc gttgatacga cattccaccg catggccgcg aacgtgaact
     6601 tcttcttgct tgatcgacag cggttgaccg gcagcgatac gcagctgttc tttgatcagg
     6661 tcaacgccgg tgatcatttc tgtaaccggg tgttctacct gaatacgggt gttcatttcg
     6721 atgaaataga actcgccgtt ttcgaacagg aactcgaaag tacctgcacc gcgatagccg
     6781 atatcaacac acgctttagc gcaacgttcg ccgatgtagc gacgcagttc cggggtaatg
     6841 cccggtgctg gtgcttcttc gaccactttc tggtggcggc gctgcatgga gcagtcacgt
     6901 tccgccagat agatagcgtt gccctgaccg tcagccagta cctgaatctc gacgtggcga
     6961 ggattttcca ggtatttctc catgtaaacc atatcgttgc tgaaagcagc tttcgcttcc
     7021 gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc cgcgcactac gcgcataccg
     7081 cgaccaccgc cgccgccgga ggctttgata atcaccggat aaccaatgcg tttagcaatg
     7141 gcacggtttt tatccatatc gtcgcccagc gggccgtcag aacccggtac gcaagggacg
     7201 cccgcttttt tcatcgccgc gattgcggat actttgtcgc ccatcaggcg aatggtttct
     7261 gctttcgggc caatgaagat aaagccggag cgttcaacct gctcggcaaa gttggcgttc
     7321 tcggagagga agccgtaacc cggatggatt gctactgcgc cggtgatttc agcggcgctg
     7381 atgattgccg ggatgttcag ataacttttt actgacggtg cagggccaat acagaccgtt
     7441 tcatctgcca gtaatacgtg ttttagatcg cgatccgcgc tggagtgcac agcgacagtc
     7501 ttgatgccca gttctttaca ggcacgaaga atacgcaatg caatctcgcc gcggttggca
     7561 ataacaattt tatccagcat gttcgcctcg ttactcgatg acgaccagcg gctcgtcaaa
     7621 ttctaccggt tgtccacttt cgaccagaat tgctttcacg gtaccggatt tgtccgcttc
     7681 gatctggttc atcattttca tggcttcaac gatgcacagg gtatcgccca cgttgacttt
     7741 ctgacccact tcgatgaacg cttttgcgtc cgggcttggg gtgcggtaga aagtaccaac
     7801 catcggggaa cgtacgatgt gaccactgat ttccgctgct gctggcgctt ccatggaagg
     7861 aacggtcgcc ggagcggctg cgttagattg agctggctgc tgcatcattg gtgcagcgta
     7921 agcttgttgc atcacaggga aacttgcggc aggagctgca cggctaatgc gtactgactc
     7981 ttcgccttca gaaatttcca gttcggagat gcctgattct tcaaccagct cgatcagttt
     8041 tttaatctta cgaatatcca tgagtgggtt ccgtactctt tgtttagtgt gattgtgaca
     8101 ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc tgtatgcata gcgataaatt
     8161 ccagcaggcc gggacgccgg tctattttgc cccagagtgt caatgttaga cttgacggac
     8221 attgtgcagc gatctgctgc tatcctccgg caaaaaacaa aatatacctt cgttgcgcca
     8281 atgtcacctt ttccgcagcg caaaaactgc gtcagggagg acgaggtcgc acattataac
     8341 gatttcgtag caattggcag ctaaatactg gtcttatcag ggaagataat caacagctaa
     8401 catgtaaata accttcaata ccgtgtaatt tgcaacaagc cgcacaattc acgaaattag
     8461 cgccatcatc gacggaactt cttataacgt aaggtgaaaa gctgtaaagc cagccctgcg
     8521 aagatgaggg gctgcaatga gataatcttc acagaccaca aataatgtag ggatgccagg
     8581 atcgcgacaa gatagacgac attacgcagc ggttgctggg gattacccaa ttttcactgc
     8641 atcgtctgga tagatgtgaa tgctaaagca cgcaaaatta accgattgac aatgctcagc
     8701 attaaatagg ttcgagtgat taatttgtag tcaaacaaaa tcagattatt caccccatgg
     8761 taccagaaat aagtaagggg ttaagcgtag cgtcgcccag gcaaagcacc ataaacctaa
     8821 caggcgacgg aggcgtatca ataatagctg tttagtggag tatcaatgcg catcaattta
     8881 tggtcagtcg aaatcatgac tttccagttt ggatgatgtt gtacaacttc atgaccaagg
     8941 tgttctacga aagctgtccg gctaaccgct tttcccgtta accgattgaa taatgatttc
     9001 aaaatgagtt ccctctctta ttattccttg ctaaatggtt agttaacctt caccagcgtg
     9061 cgaccttgga tctggttatt aatgatggcc tcggcaaagt tcggtgcctc tgacagagat
     9121 atctcttttg ccgcctgggt atagaatgat tccggtaaat cggcgaccag tcgctgccag
     9181 gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca ccccttgcaa acggacatta
     9241 cgcagaataa atggcatgac cgtggttggc agagtaaaac cacccgccag accacaggcc
     9301 gccacgcagc cgccgtaatt catttgcgcc agcacttttg ccagcacttt gtcgccaaca
     9361 gtgtcaattg ccccagccca gacttgtttt tccagaggac gggattcggc aaactcatca
     9421 cgagggagaa tacggctggc acctaaactt ttcagatatt catgggtact ttcgcgaccg
     9481 gaaacggcaa cgacctgata acccaactta tgcagcagcg ccacggcggt actgccgacg
     9541 ccaccactgg cacccgtcac gacaatctcc ccgtcctgcg ggcgaacacc ggcatcttcc
     9601 agcgccatca cacacagcat ggcggtaaaa ccggcagtac cgataatcat tgctttacgc
     9661 gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt tcactcgcgc ctgctccgcc
     9721 agcccacccc agtggttttc accaacgccc cagccagtga gtaacacctc ctgaccggca
     9781 tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa aatcgatccc aggaatcatc
     9841 ggaaaattac ggatgatttt tcccttaccg gtaatcgcca gcgcatcttt atagttcagg
     9901 ctcgaccagt gaacatcgac cgtgacatcg ccctccggca ggcgactttc gtccagagtc
     9961 tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa gtaacgcctg cataagtggt
    10021 cctcatgtgc atgatggatt ggaaaataat ttctgaagac tatactcgct aatggaaaag
    10081 caaagccgat gacgcgcaat aaattgcgag ataaatctga tttgctagta tgcccgcttc
    10141 ctcactatcg gagttaacac aaggatgaga ttaacgacga aattttcggc ctttgttacg
    10201 ctgctcaccg ggttaacaat ttttgtgact ttgctgggct gttcgctaag tttctacaac
    10261 gccattcagt ataagtttag tcatcgcgtt caggcggtgg cgacggcgat tgatacccac
    10321 cttgtgtcga atgacttcag cgcattaagg ccacaaatta ccgaattaat ggtgtcagca
    10381 gatatcgttc gtgtagacct gctgcatggt gataaacagg tttataccct ggccagaaat
    10441 ggtagttatc gtccggttgg caccagcgat ctgtttcgtg aactgagcgt tccgttgata
    10501 aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc cgatgggcaa ctatttccat
    10561 tcgttgatga ccaccgcgcc gctcacgggg gcgattggct ttatcattct tatgctcttt
    10621 ctggcggtac gctggttaca acggcaactt gccgggcaag aattgctgga aacccgggct
    10681 actcgtatct taaacggtga gcgtggctct aatgtgttgg gaaccatcta tgaatggccg
    10741 cccacaacca gcagtgcgct ggatacgctg cttcgtgaaa ttcagaacgc acgcgaacaa
    10801 cacagccgtc ttgatacgct gatccgctct tatgccgcac aggacgtgaa aaccggcctc
    10861 aataaccgac tctttttcga taatcagtta gcaacgttac tggaagatca ggagaaagta
    10921 ggtacccacg ggatcgtgat gatgattcgt ctgccggatt tcaatatgtt gagcgatacc
    10981 tgggggcaca gccaggttga agaacagttc ttcactctga cgaatctgct gtcgacattt
    11041 atgatgcgct accctggcgc actgctggcg cgttaccacc gcagtgattt tgctgcgctg
    11101 ttaccgcacc ggacgttaaa agaggcagag agcatcgccg gtcagttaat caaagccgtc
    11161 gataccttgc cgaacaataa aatgctcgat cgcgacgata tgatccacat tggtatctgc
    11221 gcctggcgta gtggtcagga taccgagcag gtaatggaac atgcagagtc tgccacgcgt
    11281 aatgcgggat tgcagggcgg caatagctgg gctatttacg atgactcgtt gcctgaaaaa
    11341 ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa tgctcagtcg cggcggcccg
    11401 cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg ttcatcatcg cgaactcatg
    11461 tgccgcatct ttgatggtaa tgaagaggtt agctcggcag agtatatgcc gatggtcttg
    11521 cagtttggct tatcggaaga gtatgaccgt ctgcaaatca gccgtcttat tccactattg
    11581 cgttactggc cagaggaaaa tctggcgatt caggttaccg ttgagtcgct gattcgcccg
    11641 cgttttcagc gttggctgcg cgatacgtta atgcaatgtg aaaaatcaca acgaaaacgc
    11701 ataattattg aacttgcaga ggccgatgta ggtcaacata tcagtcgttt acaacctgtt
    11761 attcgtttag tgaatgcttt aggggtacgg gtagccgtca accaggctgg tttgacgctg
    11821 gtaagcacca gttggatcaa agaacttaat gttgagttac tcaagctcca tccggggctg
    11881 gtcagaaaca ttgagaagcg aacggagaac cagctgctgg ttcaaagcct ggtggaagcc
    11941 tgctcaggga ccagcaccca ggtttacgcc accggtgtgc gttcgcgaag cgagtggcag
    12001 accctgattc agcgcggtgt tacaggcggg caaggggatt ttttcgcgtc ctcacagcca
    12061 cttgatacta acgtgaaaaa atattcacaa agatactcgg tttaacctgc cgtttaatcc
    12121 gttttcacgt agaataacgc gcgctgcgtc tcatgggagt gtgcttgtct gctcgccaga
    12181 ttgttgcagc acatatgcag atgaatgacc ttacgcggtt gcaaacaggc gaggaatgct
    12241 gctgatgcat taagcctttc tggactcagg cagagatttg taacaaagga aacgaactgc
    12301 actaattttc accgtagcag atgatttttg cgccttgtcg ctgctgcgtg tggttggtaa
    12361 agtaagcgga ttttcttttc cgccccagct ttcaggatta tcccttagta tgttgaaaaa
    12421 atttcgtggc atgttttcca atgacttgtc cattgacctg ggtactgcga ataccctcat
    12481 ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc gtggtggcca ttcgtcagga
    12541 tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat gacgcgaagc agatgctggg
    12601 ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa gacggcgtta tcgccgactt
    12661 cttcgtgact gaaaaaatgc tccagcactt catcaaacaa gtgcacagca acagctttat
    12721 gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc gcgacacagg ttgaacgccg
    12781 cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa gtcttcctga ttgaagaacc
    12841 gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa gcgaccggtt ctatggtggt
    12901 tgatatcggt ggtggtacca ctgaagttgc tgttatctcc ttgaacggcg tggtttactc
    12961 ctcttccgta cgcattggtg gtgaccgttt cgacgaagct atcatcaact atgtgcgtcg
    13021 taattacggt tctctgatcg gtgaagccac cgcagaacgt atcaagcacg aaatcggttc
    13081 ggcttatccg ggcgatgaag tccgtgaaat cgaagttcgt ggccgtaacc tggcagaagg
    13141 tgttccacgc ggttttaccc tgaactccaa cgaaatcctc gaagcactgc aggaaccgct
    13201 gaccggtatt gtgagcgcgg taatggttgc actggaacag tgcccgccgg aactggcttc
    13261 cgacatctcc gagcgcggca tggtactcac cggtggtggc gcactgctgc gtaaccttga
    13321 ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt gctgaagacc cgctgacctg
    13381 tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac atgcacggcg gcgacctgtt
    13441 cagcgaagag taatcggatg caggcagggg aagcgtctgt ttaccctgcc tggtctgata
    13501 cgagaatacg cataacttat gaagccaatt tttagccgtg gcccgtcgct acagattcgc
    13561 cttattctgg cggtgctggt ggcgctcggc attattattg ccgacagccg cctggggacg
    13621 ttcagtcaaa tccgtactta tatggatacc gccgtcagtc ctttctactt tgtttccaat
    13681 gctcctcgtg aattgctgga tggcgtatcg cagacgctgg cctcgcgtga ccaactagaa
    13741 cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa acagtgaact gctgatgctt
    13801 ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc tgggttcccc gctgcgtcag
    13861 gatgagcaga aaatggtgac tcaggttatc tccacggtta acgatcctta tagcgatcaa
    13921 gttgttatcg ataaaggtag cgttaatggc gtttatgaag gccagccggt catcagcgac
    13981 aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga ccagtcgcgt gctgctgatc
    14041 tgtgatgcga cccacgcgct gccaatccag gtgctgcgca acgatatccg cgtaattgca
    14101 gccggtaacg gttgtacgga tgatttgcag cttgagcatc tgccagcgaa tacggatatt
    14161 cgtgttggtg atgtgctggt gacctccggt ctgggcggtc gtttcccgga aggctatccg
    14221 gtcgcggttg tctcttccgt aaaactcgat acccagcgcg cttatactgt gattcaggcg
    14281 cgtccgactg caggactgca acgtttgcgt tatctgctgc tgctgtgggg ggcagatcgt
    14341 aacggcgcta acccgatgac gccggaagag gtgcatcgtg ttgctaatga acgtctgatg
    14401 cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg ggccaaagtt acctgaaccg
    14461 gcaacgggga tcgctcagcc gactcctcag caacccacga caggaaatgc agctactgcg
    14521 cctgctgcgc cgacacagcc tgctgctaac cgctctccac aaagggctac gccgccgcaa
    14581 agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat agtggcgagc tatcgtagcc
    14641 agggacgctg ggtaatctgg ctctctttcc tcattgcgct gttgctgcaa atcatgccct
    14701 ggccggacaa cctgattgtt ttccggccaa actgggtgtt actcatcttg ttgtattgga
    14761 tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt tgtgatgggt gccatactgg
    14821 atctgatcag cggctcgacg cttggcgtac gcgtattggc gatgagcatc attgcttacc
    14881 tggtggcgct gaaataccag cttttccgca acctcgcatt atggcagcag gcgctggtcg
    14941 tcatgttgct ttcgctggtg gtggatatta ttgttttctg ggcagagttt ttagtgatta
    15001 acgtctcttt cagaccggaa gtgttctgga gtagtgtagt caatggggtg ctctggccgt
    15061 ggattttctt gctgatgcgt aaagtccgtc agcagtttgc agtgcaataa aggtttctat
    15121 gacttctctg tatttagctt ccggttctcc gcgtcgtcag gagttacttg cgcaacttgg
    15181 cgtgaccttt gaacgtattg ttacgggcat tgaggagcag cgtcagccgc aggagagcgc
    15241 gcagcagtat gttgtgcgtc tggcgcgcga gaaagcacgg gcaggtgtcg cgcaaacggc
    15301 gaaggatctc ccggtactgg gtgcggatac tatcgttatc ctgaacggag aagtgctgga
    15361 gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc aaattatcgg gtcagaccca
    15421 tcaggtgatg acggcagtgg cgttggccga cagccagcac attctcgatt gcctggtggt
    15481 caccgatgtg actttcagaa cgttaacaga cgaagacatc gcgggctatg tcgccagcgg
    15541 tgaaccgtta gataaagcag gtgcatacgg tattcagggg ctgggtggct gttttgtcag
    15601 gaagataaat ggcagctatc acgccgtagt cggcttaccg ctggttgaaa cgtatgaatt
    15661 attaagtaat tttaacgcac tgcgtgagaa aagggataaa catgacggct gaattgttag
    15721 taaacgtaac gccttcggaa acgcgagtgg cgtatattga tggcggtatt ctgcaggaaa
    15781 ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa tatctacaag ggtcgtgtaa
    15841 gtcgtgtact tccgggtatg caggcggctt ttgtagatat tgggctggat aaagccgcgt
    15901 ttcttcatgc atctgacatc atgccgcaca ccgaatgtgt ggcgggtgaa gaacaaaagc
    15961 aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg gcaagatctg atggtgcagg
    16021 tggtgaaaga tccgcttggc actaaggggg cgcgcctgac caccgatatc acgctgcctt
    16081 ctcgctatct ggtctttatg ccaggggctt ctcacgttgg tgtttcccaa cgtattgaaa
    16141 gcgaatcaga acgtgaacgc ctgaaaaaag tggtcgcgga gtattgcgac gagcagggcg
    16201 ggtttatcat ccgtactgca gcggaagggg ttggcgaggc tgaactggcc tccgatgccg
    16261 cttatctgaa acgcgtctgg accaaagtta tggagcgtaa aaagcgcccg cagacccgtt
    16321 atcagctgta cggcgaactg gcgctggcgc agcgtgttct gcgtgatttc gccgatgccg
    16381 aactggaccg cattcgcgtt gactcacgcc tgacttacga agcattactt gagttcacct
    16441 cggagtacat tcccgagatg accagcaagc tggagcatta caccggacgc cagccgattt
    16501 tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgct ggaacgcaaa gtagaactga
    16561 aatccggtgg ttatctcatt atcgaccaga ccgaagcgat gaccaccgtg gacatcaata
    16621 ccggggcgtt tgtcggtcat cgcaatctgg acgacaccat tttcaatacc aatattgaag
    16681 cgacgcaggc tatcgctcgc cagttacggt tgcgcaatct gggcgggatt atcattattg
    16741 atttcatcga tatgaataat gaagatcacc gccgccgcgt gctgcattcg ctggagcaag
    16801 cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt ttcggcgctg gggctggtgg
    16861 agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt actgtgtaac gaatgcccaa
    16921 cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt atgctatgaa atcatgcgcg
    16981 agattgttcg tgtccaccat gcttacgact ccgaccgttt cctggtctat gcttctccgg
    17041 cagtagctga agccttgaaa ggcgaagagt cacactcgct ggcggaagtg gaaattttcg
    17101 ttggtaaaca ggttaaagta caaattgaac cgctctataa ccaggagcag tttgacgtcg
    17161 taatgatgta aacagatgct gggccgccat ccggcaaagg gtttttgagt cacatattta
    17221 gcagacaagg agtgacgggt gaggcgattg ccggggattt tactgcttac tggagccgcg
    17281 ctcgttgtga tcgctgccct gctggttagc ggcctgcgta ttgctttacc gcatcttgac
    17341 gcctggcgtc cggaaatcct caacaaaata gaatccgcga ctggcatgcc ggttgaagcc
    17401 agtcagctct cagccagctg gcagaatttt ggcccgacgc ttgaagcaca cgacatccgt
    17461 gcagaactaa aagatggcgg cgaattttcg gttaaacgcg ttactctggc gctggatgtc
    17521 tggcagagcc tgttacatat gcgctggcag tttcgcgacc tcactttctg gcagctgcgc
    17581 tttcgcacca acactcctat caccagcggt ggtagcgatg acagtctgga agccagtcac
    17641 atcagcgatc tgtttcttcg tcaatttgac catttcgatc tccgcgacag tgaagtcagt
    17701 ttcctgacgc catccggtca gcgcgccgag ctggcgatcc cacaactcac ctggctgaac
    17761 gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct ccagccttac cggacagcac
    17821 ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg ggttgttaag caatggtcgc
    17881 gtctggctcc aggcggatga catcgacctg aagccgtggc tcggtaaatg gatgcaggac
    17941 aatattgcgc tggaaacggc acagttctcc cttgaaggct ggatgacgat cgacaaaggc
    18001 gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg ccagctggtt gggcgagaag
    18061 caaacgcata cgctgtcggt ggataatctg accgcgcata ttacgcgtga aaatccgggc
    18121 tggcagttct ctattcccga tacacggatc acgatggacg gcaaaccctg gccgagcgga
    18181 gcattgacgc tggcctggat accggaacag gacgttggcg gcaaagacaa taaacgcagt
    18241 gacgaactcc ggattcgcgc cagtaatctg gagctggcag gcctggaggg catacgcccg
    18301 ctggccgcga aactttcacc tgcactgggt gatgtttggc gctccacaca accgagcggc
    18361 aagattaaca cgctggcgct ggatatcccg cttcaggcgg cagacaagac ccgttttcag
    18421 gcatcgtgga gcgatctggc ctggaagcaa tggaaattat taccgggtgc ggaacacttc
    18481 tccgggacgc tttccggcag cgttgaaaat ggtttgctta ccgcgtcgat gaagcaggca
    18541 aagatgcctt acgaaacggt attccgtgcg ccactggaaa tcgccgacgg ccaggcaact
    18601 ataagctggc tgaacaatga caaaggtttc cagctggatg ggcgtaatat tgacgttaaa
    18661 gccaaagccg tccatgcgcg cggcggtttt cgttacctgc aacctgctaa cgatgaaccc
    18721 tggctgggta ttctggctgg catcagtacc gatgatggtt cacaagcctg gcgctatttc
    18781 ccggaaaact tgatgggtaa agacctggtt gattacttaa gtggcgcgat tcagggcggt
    18841 gaagcggata acgcgacgct ggtttatggt ggcaatccgc aactcttccc ctataaacac
    18901 aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg cgaagtttgc cttccagccg
    18961 gactggcctg cattaactaa ccttgatatt gaactggact ttattaacga cggtttatgg
    19021 atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga gtaatctcac cgcagtgatc
    19081 cctgactact cgaaagaaaa actgctgatt gacgctgaca ttaaaggtcc gggtaaagcc
    19141 gttggccctt actttgatga gacaccgctg aaagattctc tgggtgcgac cctgcaagaa
    19201 ctccagctcg acggcgatgt gaatgctcgc ttacatcttg atatcccgct gaacggcgaa
    19261 ctggtaaccg cgaaaggtga tgtgacgctg cgtaataaca gtctgtttat caaaccactc
    19321 gacagcaccc tgaaaaattt gagcggtaaa ttcagcttta tcaatggcga tctgcaaagt
    19381 gaaccactga cagcaagctg gtttaatcag ccgttgaacg tggatttttc caccaaagaa
    19441 ggggcaaaag cctaccaggt cgcggtgaat ctcaacggta actggcaacc agcgaaaacc
    19501 ggcgttctgc ctgcagcggt gaacgaagca ttgagtggca gcgtggcgtg ggatggtaaa
    19561 gtgggcattg atctgcctta tcatgctggc gcgacctata acgtagagct gaacggcgat
    19621 ctgaagaatg tgagcagtca cttaccttca ccgttagcca aacctgcggg tgaaccacta
    19681 gcggtaaacg ttaaggttga tggcaatctc aacagctttg aattaaccgg acaggctggt
    19741 gcggataacc atttcaatag ccgctggttg ctcggtcaaa agctgacgct cgatcgtgct
    19801 atttgggcgg cagacagtaa aacgctcccg ccgttgccgg aacaaagtgg tgttgaactc
    19861 aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt ttcagaaagg tgcggcggag
    19921 agtgtcggtg gtgcagcgag tttcccacaa cacataacgt tacgtacgcc tatgttgtcg
    19981 ctgggaaatc agcaatggaa taacctgagt attgtttcgc aaccgacggc aaatggcacc
    20041 caggttgagg cgcaagggcg tgaaatcaac gccacgctgg cgatgcgtaa taacgcgccg
    20101 tggctggcga atatcaaata tctttattac aacccgagcg tggcgaaaac tcgtggtgat
    20161 tcaacgccgt catcaccttt cccgacaacg gagcgcatta acttccgtgg ctggccggac
    20221 gcacaaatac gatgcgcaga gtgctggttc tgggggcaaa aattcggtcg tattgacagt
    20281 gatctcacca tttctggcga tacattaacg ctgaccaatg gactgattga taccggtttc
    20341 tcgcggctta ctgccgatgg tgaatgggtt aataatccgg ggaatgaacg tacctcgctg
    20401 aaaggaaaac tgcgcgggca gaaaattgat gccgccgcag aattttttgg tgtcacgacg
    20461 cccatacggc agtcgtcatt taatgtggat tacgatttac actggcgtaa agcaccgtgg
    20521 cagccagatg aggcgacgtt gaatggcatc attcatactc aactgggtaa aggcgaaatt
    20581 accgaaatca ataccggaca tgccgggcaa ttgctgcgct tattgagcgt agatgccctg
    20641 atgcgtaagc tgcgttttga tttcagagac acttttggcg aagggttcta ttttgactcc
    20701 attcgcagca ccgcgtggat taaagacggc gttatgcaca ccgacgacac gttggtggat
    20761 ggcctggagg cggatatcgc catgaaaggg tcggtaaatc tggtacgtcg cgacctgaat
    20821 atggaagcgg ttgtcgcacc agagatttct gcgacggtgg gcgtggctgc ggcttttgcg
    20881 gttaacccca ttgttggcgc ggcagtgttt gctgccagta aagtgctggg gccgctgtgg
    20941 agcaaagtct ccattttgcg ctatcacatt tcgggtccgc tggacgatcc gcaaatcaac
    21001 gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat ttgacgaggg cgcgtaattg
    21061 ccccaatctc ataagataat cgttgccaaa ggccaacgag ccagaacata accgtaggcc
    21121 ggatagggcg tttacgccgc atccggcagc cgttgcctga tgcgacgctg tgcgtcttat
    21181 caggcctaca aacggaacat aaccgtaggt cggataaggc gtttacgccg catccgacag
    21241 ccgttgcctg atgcgacgct gtgcgtctta tcaggcctac aaaccggaac ataatcgtag
    21301 gccggataag gcgtttacgc cgcatccggc agccgtaaaa aatcctctac tgcagtaact
    21361 aacgagtagc aaaaacgatg agtctgaacc tggtaagtga acaattgcta gcggcgaacg
    21421 gcctgaaaca tcaggacttg ttcgcgatcc tcggtcaact ggccgaacgt cgccttgatt
    21481 atggcgatct ctattttcag tcgagctatc acgaatcctg ggttttagaa gaccgcatta
    21541 ttaaagatgg ttcttacaac atcgatcagg gcgttggcgt gcgtgcaatc agcggtgaaa
    21601 aaaccggatt tgcttacgct gaccaaatca gcctgctggc gctggaacag agtgcgcaag
    21661 cggcgcgcac catcgtccgt gatagtggtg atggcaaagt acagacgctg ggcgcggtag
    21721 agcatagccc gttgtatacc tcggtagatc cgctgcaaag catgagccgt gaagagaagc
    21781 tggatatcct gcgtcgcgtc gataaggttg cccgcgaagc ggacaagcgc gtacaggaag
    21841 tgactgccag cctcagtggt gtctatgaat taattttggt tgcggccacc gacggcacgc
    21901 tagcggcgga tgtccgtccg ctggtgcgtc tttccgtgag cgttctggtc gaagaagatg
    21961 gcaaacgcga acgcggtgcc agtggcggcg gcggtcgttt tggttatgag ttcttccttg
    22021 ccgatctcga cggcgaagtc cgtgcggatg catgggcaaa agaagcagtg cgtatggcgc
    22081 tggtcaatct ttctgccgtt gctgcaccag cgggcaccat gccggtagta cttggcgcag
    22141 gttggccggg cgtgctgttg catgaagcgg ttggtcacgg tctggaaggc gacttcaacc
    22201 gccgtggcac ttcagtattt agtggacagg tcggggagct ggtggcttca gaactgtgta
    22261 ccgtggttga tgacggcgcg atggtcgatc gccgtggttc ggtggcgatt gatgacgaag
    22321 gtacgccagg ccagtacaac gtgctgattg agaacggcat tctgaaaggc tacatgcagg
    22381 ataaactcaa cgcgcgtttg atggggatga cgccgactgg caacggtcgc cgtgaatcct
    22441 acgcccatct gcccatgccg cgtatgacca acacctatat gctgccgggt aaatcgaccc
    22501 cgcaggaaat tattgaatcc gttgagtacg gtatctatgc accgaacttt ggtggcggtc
    22561 aggtggatat cacctccggc aaattcgttt tctccacttc agaagcatat ctgattgaaa
    22621 acggtaaagt aacgaagccg gtgaaaggcg caacgttgat tggttccggt atcgaaacca
    22681 tgcagcagat ttcgatggtt ggcaacgacc tgaaactgga taacggcgtg ggtgtctgcg
    22741 gtaaagaagg gcaaagtttg ccggttggcg tgggccagcc aacgttgaaa gtcgataacc
    22801 tgactgttgg cggtactgcg taataatttc agtattttca gaaggataat taatctttct
    22861 acgtgcacga acggtcccct cgctcctctg gggttagggt gaggggaacc cgttggcaca
    22921 ggtttgtaca acgtaacagt acaagatgaa ttacttctct ttcccgcgcc cgtgcatctc
    22981 ctgaaacaat ttaccgacct caacaaaata atccgtcagc gagttgatca cgacctgtac
    23041 cttcagcggc agcttatctt tttcggtata taacgcataa accgggcgtg gatctgactg
    23101 ataacgcggc agcaggatct ccagctcccc acgattgatc tcgttgatca cccacatcag
    23161 cggcacgtag gcgatcccgg cacccgccgt tagccagcgc accagcgtca tcggatcatt
    23221 agtcacaaat ctcccttgcg ggatcaggcg agtcgagatc ccttccggtg caatcagttc
    23281 aaattcattg tcgggccgca cgctgtattc aagccatgaa tggctactta aatcggcggg
    23341 tttttccggt atgccgtatt gtgtgagata gcttttcgcg gcgcacacca ccattggcat
    23401 cgcgccaaga cggcgggaaa acaggctgga atcctgcaac gcgccgacgc ggatcaccac
    23461 atccagaccg tcggcaatca ggtcgggggc tggaattccg gtaaccagat tgacgctcaa
    23521 acctgggtat tctttcagca ttttggctgt cagcccggcg agaacatttt gtgccatagt
    23581 tgaagaacag ccaatgcgta gcgtcccgat gggggtgtta ttgaaggcat acagttgctc
    23641 atgaacatcc tgcacttcat gaagcatacg acggcagccc tggtagtaaa ttctaccggc
    23701 ttcggtcagg ccaatgctgc gtgtgctacg gtttaacagc tttacctgca actcatcttc
    23761 cagttttgac accgtctgac tgatggacga aacgctcatc tgtagctgtc tggcggcggc
    23821 ggtaaaagag ccaaattcaa ctactttggc aaacaccgac atgcgtttta gtcgttccat
    23881 tattcactct gacttaaaag tgatttagat cacataatat agataacagc ataacagtta
    23941 cgctaatata ttaaatatca atctacagca atgttgctct cgcccggctt tccatgccct
    24001 tctctgcggc gacagatgct gaaaataata acgcctgctc tctctataca accaaggtca
    24061 acatgagtct gtttcccgtt atcgtggtgt ttgggctgtc cttcccaccg atattttttg
    24121 aattgctttt atcactggcg attttctggc tggtgcgccg ggtacttgtg ccaacaggta
    24181 tctacgactt tgtctggcat ccggcgttgt tcaacaccgc gctctattgc tgcttgtttt
    24241 atttgatatc gcgactgttc gtttgaggtt gaagtgaaaa cactaataag aaaattctcc
    24301 cgtacggcca tcacggtcgt attagtcatt ctggccttca tcgcaatttt taatgcctgg
    24361 gtctattaca ccgaatcccc gtggacgcgt gacgcgcgct ttagcgctga cgtcgttgcg
    24421 atcgcgccgg acgtttctgg actcattacc caggtgaatg ttcatgataa ccagctggtg
    24481 aaaaaaggac aggtactgtt caccatcgac cagccgcgct atcaaaaggc gcttgaggaa
    24541 gcgcaagccg atgttgctta ttatcaggta ctggcacagg agaaacgcca ggaggccgga
    24601 cgtcgtaacc gtctcggtgt gcaggcgatg tctcgcgaag agatcgacca ggccaacaac
    24661 gtactacaaa cggttctgca tcagttagcg aaagcgcaag cgacccgcga tctggcaaaa
    24721 ctggatcttg aacgcacggt gatccgcgcg ccagcggatg gctgggtgac caacctcaac
    24781 gtctataccg gtgagtttat tactcgtgga tcaacggcgg ttgcgctggt gaaacagaac
    24841 tctttctatg tactggccta tatggaagaa actaagctgg aaggggtgcg ccctgggtat
    24901 cgtgcagaga tcacgccgct tggcagtaac aaagtgctga aagggactgt tgatagtgtt
    24961 gccgctgggg tcaccaacgc cagcagcacg cgtgacgaca aagggatggc gaccatcgac
    25021 tctaaccttg aatgggtgcg tttggcgcaa cgtgttccgg ttcgtattcg tctcgacaac
    25081 cagcaagaga acatctggcc tgcgggcacc actgctacag tggtggtcac tggcaaacaa
    25141 gatcgcgacg aaagccagga ttcgttcttc cgtaaaatgg cccatcgcct gcgtgagttt
    25201 ggttaatcac gatgggtatt ttctccattg ctaaccaaca tattcgcttt gcggtaaaac
    25261 tggcgaccgc cattgtactg gcgctgtttg ttggctttca cttccagctg gaaacgccac
    25321 gctgggcggt actgacagcg gcgattgttg ccgccggtcc ggcctttgct gcgggaggtg
    25381 aaccgtattc tggcgctatt cgctatcgtg gctttttgcg catcatcggc acatttattg
    25441 gctgtattgc cggactggtg atcatcattg cgatgatccg cgcaccatta ttgatgattc
    25501 tggtgtgctg tatctgggcc ggtttttgta cctggatatc ctcgctggta cgaatagaaa
    25561 actcgtatgc gtgggggctg gccggttata ccgcgctgat cattgtgatc accattcagc
    25621 cggaaccatt gcttacgccg cagtttgccg tcgaacgttg tagcgagatc gttatcggta
    25681 ttgtgtgtgc gattatggcg gatttgctct tttctccgcg atcgatcaaa caagaagtgg
    25741 atcgagagct ggaaagtttg ctggtcgcgc aatatcaatt aatgcaactc tgtatcaagc
    25801 atggcgatgg tgaagttgtc gataaagcct ggggcgacct ggttcgacgc accacggcgc
    25861 tacaaggcat gcgcagcaac ctgaatatgg aatcttcccg ctgggcgcgg gccaatcgac
    25921 gtttaaaagc gatcaatacg ctatcgctga cgctgattac ccaatcctgc gaaacttatc
    25981 ttattcagaa tacgcgcccg gaattgatca ctgatacttt ccgcgaattt tttgacacgc
    26041 cggtagaaac cgcgcaggac gtccacaagc agctcaaacg cctgcgaaga gttatcgcct
    26101 ggaccgggga acgggaaacg cctgtcacca tttatagctg ggtcgcggcg gcaacgcgtt
    26161 atcagcttct caagcgcggc gttatcagta acacaaaaat caacgccacc gaagaagaga
    26221 tcctgcaagg cgaaccggaa gtaaaagtag agtcagccga acgtcatcat gcaatggtta
    26281 acttctggcg aaccacactt tcctgcattc tgggcacgct tttctggctg tggacgggct
    26341 ggacttccgg tagtggtgca atggtgatga ttgcggtagt gacgtcactg gcaatgcgtt
    26401 tgccgaatcc acgcatggtg gcgatcgact ttatctacgg gacgctggcc gcgctgccgt
    26461 tagggctgct ctactttttg gtgattatcc ctaataccca acagagcatg ttgctgctgt
    26521 gcattagcct ggcagtgctg ggattcttcc tcggtataga agtacagaaa cggcgactgg
    26581 gctcgatggg ggcactggcc agcaccataa atattatcgt gctggataac ccgatgactt
    26641 tccatttcag tcagtttctc gacagcgcat tagggcaaat cgtcggctgt gtgctcgcgt
    26701 tcaccgttat tttgctggtg cgggataaat cgcgcgacag gactggacgt gtactgctta
    26761 atcagtttgt ttctgccgct gtttccgcga tgactaccaa tgtggcacgt cgtaaagaga
    26821 accacctccc ggcactttat cagcagctgt ttttgctgat gaataagttc ccaggggatt
    26881 tgccgaaatt tcgcctggcg ctgacgatga ttatcgcgca ccagcgcctg cgtgatgcgc
    26941 cgatcccgat taacgaggat ttatcggcgt ttcaccgaca aatgcgccgc acagcagacc
    27001 atgtgatatc tgcccgtagc gatgataaac gtcgtcggta ctttggtcag ttgctggaag
    27061 aactggaaat ctaccaggaa aagctacgca tctggcaagc tccaccgcag gtgacggaac
    27121 cggttcatcg gctggcgggg atgctccata agtatcaaca tgcgttgacc gatagttaag
    27181 tcaaaaccga cgccaaaagc gtcggttttt tcatggctat acttagcgat gaacggcaga
    27241 actcgccgcg aaacgtgacg gtggcaacag atgaatattt atacctttga ttttgatgag
    27301 attgagagtc aggaggattt ttatcgcgac ttcagccaag cctttggtct ggcgaaagat
    27361 aaggtacgcg atctcgactc actatgggat gtgttaatga acgatgtcct gccgctacca
    27421 cttgagattg aatttgttca tctgggagag aaaacgcgtc gccgttttgg cgcgttaata
    27481 ttgctgtttg atgaggcaga ggaagagctg gaagggcatt tgcgttttaa tgttcgtcat
    27541 tagcgcaaaa aaaagccccc gaaccggggg caatatcgtc ggacaagacg atgagggttt
    27601 atttgtacag ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt
    27661 aggccgttgc gcctttctct tccgcttttt tgttcagcat ttcacgcata tccattggcg
    27721 aagacgccac accacttacg gatacggtcc cgattgcttc acgattttgt gcttgtgcag
    27781 catcaatgga gtcggcagcg aatgcaccga aagagagaac agaaagtacg cttaatgcag
    27841 caacagtggt tttgattttc atgattttta cctcgacata atcttttagc tgggcctttg
    27901 tttcgtgacc catatcacaa aatcaagtat acactaatta ttggactaat taataccacg
    27961 ctaactgttt ctgttgatac taagtgttaa aaatgt
//
LOCUS       DAZFKF010000064        27130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFKF010000064 DAZFKF010000000
VERSION     DAZFKF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.064"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(295..2124)
                     /gene="glmS"
                     /locus_tag="ACHKA1_003200"
     CDS             complement(295..2124)
                     /gene="glmS"
                     /locus_tag="ACHKA1_003200"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO5680811.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2286..3656)
                     /gene="glmU"
                     /locus_tag="ACHKA1_003201"
     CDS             complement(2286..3656)
                     /gene="glmU"
                     /locus_tag="ACHKA1_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO5680812.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELQEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(4009..4428)
                     /gene="atpC"
                     /locus_tag="ACHKA1_003202"
     CDS             complement(4009..4428)
                     /gene="atpC"
                     /locus_tag="ACHKA1_003202"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO5680813.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4449..5831)
                     /gene="atpD"
                     /locus_tag="ACHKA1_003203"
     CDS             complement(4449..5831)
                     /gene="atpD"
                     /locus_tag="ACHKA1_003203"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO5680814.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRCLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5858..6721)
                     /gene="atpG"
                     /locus_tag="ACHKA1_003204"
     CDS             complement(5858..6721)
                     /gene="atpG"
                     /locus_tag="ACHKA1_003204"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO5680815.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6772..8313)
                     /gene="atpA"
                     /locus_tag="ACHKA1_003205"
     CDS             complement(6772..8313)
                     /gene="atpA"
                     /locus_tag="ACHKA1_003205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO5680816.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8326..8859)
                     /gene="atpH"
                     /locus_tag="ACHKA1_003206"
     CDS             complement(8326..8859)
                     /gene="atpH"
                     /locus_tag="ACHKA1_003206"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO5680817.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8874..9344)
                     /gene="atpF"
                     /locus_tag="ACHKA1_003207"
     CDS             complement(8874..9344)
                     /gene="atpF"
                     /locus_tag="ACHKA1_003207"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO5680818.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9406..9645)
                     /gene="atpE"
                     /locus_tag="ACHKA1_003208"
     CDS             complement(9406..9645)
                     /gene="atpE"
                     /locus_tag="ACHKA1_003208"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO5680819.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9692..10507)
                     /gene="atpB"
                     /locus_tag="ACHKA1_003209"
     CDS             complement(9692..10507)
                     /gene="atpB"
                     /locus_tag="ACHKA1_003209"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO5680820.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10516..10896)
                     /gene="atpI"
                     /locus_tag="ACHKA1_003210"
     CDS             complement(10516..10896)
                     /gene="atpI"
                     /locus_tag="ACHKA1_003210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO5680821.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11513..12136)
                     /gene="rsmG"
                     /locus_tag="ACHKA1_003211"
     CDS             complement(11513..12136)
                     /gene="rsmG"
                     /locus_tag="ACHKA1_003211"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO5680822.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(12200..14089)
                     /gene="mnmG"
                     /locus_tag="ACHKA1_003212"
     CDS             complement(12200..14089)
                     /gene="mnmG"
                     /locus_tag="ACHKA1_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO5680823.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14468..14911)
                     /gene="mioC"
                     /locus_tag="ACHKA1_003213"
     CDS             complement(14468..14911)
                     /gene="mioC"
                     /locus_tag="ACHKA1_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO5680824.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(15001..15459)
                     /gene="asnC"
                     /locus_tag="ACHKA1_003214"
     CDS             complement(15001..15459)
                     /gene="asnC"
                     /locus_tag="ACHKA1_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO5680825.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15611..16603
                     /gene="asnA"
                     /locus_tag="ACHKA1_003215"
     CDS             15611..16603
                     /gene="asnA"
                     /locus_tag="ACHKA1_003215"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO5680826.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16608..18059)
                     /gene="viaA"
                     /locus_tag="ACHKA1_003216"
     CDS             complement(16608..18059)
                     /gene="viaA"
                     /locus_tag="ACHKA1_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO5680827.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRNRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSKEWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(18053..19549)
                     /gene="ravA"
                     /locus_tag="ACHKA1_003217"
     CDS             complement(18053..19549)
                     /gene="ravA"
                     /locus_tag="ACHKA1_003217"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO5680828.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQACAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19772..21640
                     /gene="kup"
                     /locus_tag="ACHKA1_003218"
     CDS             19772..21640
                     /gene="kup"
                     /locus_tag="ACHKA1_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO5680829.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21807..22226
                     /gene="rbsD"
                     /locus_tag="ACHKA1_003219"
     CDS             21807..22226
                     /gene="rbsD"
                     /locus_tag="ACHKA1_003219"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO5680830.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            22234..23739
                     /gene="rbsA"
                     /locus_tag="ACHKA1_003220"
     CDS             22234..23739
                     /gene="rbsA"
                     /locus_tag="ACHKA1_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO5680831.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23744..24709
                     /gene="rbsC"
                     /locus_tag="ACHKA1_003221"
     CDS             23744..24709
                     /gene="rbsC"
                     /locus_tag="ACHKA1_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO5680832.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24734..25624
                     /gene="rbsB"
                     /locus_tag="ACHKA1_003222"
     CDS             24734..25624
                     /gene="rbsB"
                     /locus_tag="ACHKA1_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO5680833.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25750..26679
                     /gene="rbsK"
                     /locus_tag="ACHKA1_003223"
     CDS             25750..26679
                     /gene="rbsK"
                     /locus_tag="ACHKA1_003223"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO5680834.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANISFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLKSVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26683..>27130
                     /locus_tag="ACHKA1_003224"
     CDS             26683..>27130
                     /locus_tag="ACHKA1_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5680835.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAP"
BASE COUNT         6489 a         6944 c         6951 g         6746 t
ORIGIN      
        1 agtgataatt tattatcaca cattgttaca tagttaaata aaattacata ttgatgattg
       61 ttttacaagc gattcttgaa tgaacaaaac gtgctattca ttacaaagta cgtcaattat
      121 ggcaattgtt tgtcaatgat tcgaggtatt taattactac tacggttaaa acaagtgttc
      181 aacggtgccc caaataatag ctgaagttaa ttatatggaa tgaggttaga ttattaatgc
      241 tggtaagtaa aagggccgcc aaaaggcagc ccttcgttca gcaaatgaat aatattactc
      301 aaccgttacc gattttgcca ggttacgcgg ctggtcaacg tcggtgcctt tgatcagcgc
      361 gacgtgataa gccagtaact gcagcggaac ggtgtagaag atcggtgcaa tcacctcttc
      421 cacatgcggc atctcgatga tgtgcatgtt atcgctactt acaaaacccg catcctgatc
      481 ggcgaagaca tacaattggc cgccacgcgc acgaacttct tcaatgttgg atttcagttt
      541 ttccagcaat tcgttgttcg gcgcaacaac gataaccggc atatccgcgt caatcagcgc
      601 cagcggaccg tgtttcagtt cacctgcagc gtaggcttca gcgtgaatgt aagagatctc
      661 tttcagcttc aatgcgcctt ccagcgcgat tgggtactga tcgccacggc ccaggaacag
      721 cgcgtgatgt ttgtcagaga aatcttctgc cagagcttca atgcgtttgt cctgagacag
      781 catctgctca atacggctcg gcagcgcctg cagaccatgc acaatgtcat gttcaatgga
      841 ggcatccaga cctttcaggc gagacagctt cgccaccagc atcaacagca cagttaactg
      901 ggtggtgaat gctttagtgg atgccacgcc gatttctgta cccgcgttgg tcattagcgc
      961 cagatcggat tcgcgcacca gagaagaacc cggaacgtta cagattgcca gtgaaccaag
     1021 gtaacccagc tctttcgaca gacgcaggcc cgccagggta tctgcggttt cgccagactg
     1081 tgacaaggtg atcatcaggc tgttacgacg cacggcagat ttgcgatagc ggaattcaga
     1141 ggcgatttcg acgtcgcacg gaatacctgc cagcgattca aaccagtaac gggaaaccat
     1201 accggagtta taagaagtac cacaggcgag gatctgaata tgctcaacct tcgacagcag
     1261 ttcgtcggcg ttcggtccca gctcgcttaa atcaacctga ccgtggctga tgcgcccggt
     1321 aagggtgttt ttgatcgcgt tcggctgttc gtagatctct ttctgcatgt agtgacggta
     1381 aataccttta tcgcccgcgt catattgcag attggattcg atatcctgac gttttacttc
     1441 cgcgccagtt ttatcgaaga tgtttaccga acggcgagta atttccgcaa tatcgccctc
     1501 ttcaaggaag ataaagcgac gggtcaccgg cagcagcgcc agctggtcag aagcgataaa
     1561 gttttcgccc atacccagac caatcaccag cggactacca gaacgtgctg ccagcagggt
     1621 atccgggtga cgggagtcca tgatcactgt accgtacgca ccacgcagtt gcgggatagc
     1681 acgcagaaca gcctcacgca gagtaccgcc ctgtttcagc tcccagttca ccagatgggc
     1741 aatcacttcg gtgtcggttt cagaaacgaa ggtatagcca cgcgctttta actcttcacg
     1801 cagcggttca tggttttcga tgatgccgtt atgcaccacc acaatgtgtt cagaaacatg
     1861 cggatgcgcg ttaacttctg aaggttcacc atgggtcgcc cagcgagtgt gagcaatacc
     1921 ggtgccgcca tgcagaggat gttcttccgc tgcctgagcc agcatctgga ctttaccgag
     1981 gcgacgcagg cgggtcatat gaccttccgc atcaacaacg gccagaccgg cagagtcata
     2041 tccgcggtat tccagacgac gtaaaccttc aagaaggatt tctgctacat cacgttgcgc
     2101 gatcgcgcca acaattccac acatagtttt tgattccgat ttatatcgtt gtcggtcaac
     2161 ctgtatgccc gttttttcgg gcgccccgag ccttgtagag agtggggtta tttttatagt
     2221 tactgcttgt gggagggaga ttatgttatc tcctcatccc atgtgaccgg attacccggc
     2281 cagaatcact ttttctttac cggacgacgc cagccttctt tctgagtctg cggcacacgg
     2341 ctgatagcta atgcattttc gccgacatta cgcgtcacag ttgtacccgc agcaatggtc
     2401 gcgcctttgc ctactgttac cggggccacc agctgagtgt cggaaccgac aaacacatcg
     2461 tcgccgataa tggtcttaaa tttattcgca ccatcgtagt tgcaggtaat ggttcccgcg
     2521 ccgatgttaa cgttatcgcc aatttccgca tcgcccaggt aagtcagatg accagctttc
     2581 gagcctttac ccagacgcgc ttttttcatc tcaacaaagt taccgacgtg tgcaccttcc
     2641 tgcaactcag caccaggacg caaacgggca aacgggccaa tggtacaggc cgccgccaga
     2701 ttcgcgtctt ccacgacggt atatgggcta atttcgcaat catcgccaat cacgctgttt
     2761 ttaatcacgc aaccggtgcc gattttcacg cgatgaccga gagtcacgtt gccctcgatg
     2821 ataacgttag tatcaatttc aacatcgcgc ccgtgagtaa gcgtaccacg cagatcaaaa
     2881 cgcgccggat cgcgcagcat aacgcctgct aacagcagtt tttcagcctg ttcggactga
     2941 taaacacgct ccagacggga gagttgcagg cggttattca cgccttctac ttcgcttaaa
     3001 cgttgcggat gaacggcgac gatttcacgc ccttcctgat acgccagtgc aataatgtcg
     3061 gtgatgtagt attcgccctg agcattattg ttggtcagct tcgccagcca gcgtttcata
     3121 tctgcgccgt tagcgatcag aatgccggtg ttgatctcct gaatctgacg ctgctcgtcg
     3181 gtggcatctt tgtgctcaac aatgccggta actttgccgt tttcacgggt gatacgtcca
     3241 taaccggtcg gatcatccag tttcaccgtc agcagaccaa tgccaccctg cggtttagca
     3301 tcacgcagac gctggagtgt ttcgacagag atcagcggca cgtcgccgta gagcattaaa
     3361 atgtcttcat catcggcaaa gaaaggtgca gcctgctgca ttgcatgacc agtacccagc
     3421 tgttctgcct gaagcaccca gttcaggttg tcgtctttca gcgcctgttt tagcagatcg
     3481 ccgccgtgac cgtacaccag gtgaacgtgc gctgcgccta attcattcgc agcatcaatg
     3541 acatgctgaa ccatcgcttt cccggcaagg gtatgcagca ctttcggaag atcggaatac
     3601 atgcgcgtgc ctttacctgc ggcaaggatc actacgctca tagcattatt caacatacgc
     3661 gtcctgactg taatttgaga acgaatttaa accgcttcac cttgaaaaaa ctacattttt
     3721 ttcatcgtga aatggacaga ggataaattg ttcaataacg gattatcccc gctgacgaaa
     3781 gcgccatttt cgaccatcgt cacgcctttt gtctgcagaa aattaaggca gtaccttcat
     3841 ttaagcgtca ggtggatgtt tttgctctta tttcgatcaa tgaataaacg ggaaaacagg
     3901 gggttttata tcagcaggat ctatgtgaac gctattcagg acgggtcaca cgcgcaaaaa
     3961 aaaagccagc ctgtttccag actggctttt gtgcttttca agccggtgtt acatcgcttt
     4021 tttggtcaac tcgataacgc gcagctgcgc gatcgctttg gccagttccg cagacgcctg
     4081 agcgtaatct acgtcgccgt gagagctgct aatgtgctct tcagccttac gtttcgcttc
     4141 catggctcgc gcttcgtcga gatcctggcc gcgaattgcg gtgtcggcca gaacggtcac
     4201 gttgccaggc tgcacttcaa gaatgccgcc agacagatag ataaactctt cgtgaccgtg
     4261 ctgtttcacg atgcgaatca taccaggctt aatggcggtg agcagcggtg cgtggccagg
     4321 gtagatcccc agttcacctt cgctacccgt tacctggatt ttctcgacca gaccagagaa
     4381 catttgttgc tctgcgctga cgacgtccag gtggtaagtc attgccatat caccctccga
     4441 ttaaggcgtt aaagtttttt ggctttttcc acagcttctt cgatggaacc gaccatgtag
     4501 aacgcctgct ccggcaggtg atcgtattcg ccttccatga tgcctttaaa gccacggatg
     4561 gtgtctttca gggagacgta tttacccgga gaaccggtga atacttctgc cacgaagaac
     4621 ggctgggaca ggaagcgctg gatcttacga gcacgtgcta ccaccagttt gtcttcttca
     4681 gacagttcat ccatacccag gatggcgatg atgtctttca gttcctgata acgttgcagg
     4741 atggactgaa cgccacgcgc ggtgtcgtag tgttcctgac caaccaccag cgggtccagc
     4801 tgacggctgg tggagtccag cgggtcaacg gccgggtaga tacccagaga cgcgatctga
     4861 cggctcagta ccacggttgc gtcaaggtgc gcaaaggtgg ttgccggaga cgggtcagtc
     4921 aagtcatccg caggtacgta tactgcctgt acggaggtga tagaaccggt tttggtggag
     4981 gtgatacgtt cctgcagaac gcccatctct tccgccaggg tcggctgata acctaccgct
     5041 gaaggcatac ggcccagcag tgcggatact tccgtaccgg ccagggtgta acgatagatg
     5101 ttgtcaacga acagcagaac gtcacgacct tcgtcacgga atttctcagc catggtcaga
     5161 ccggtcagag caacgcgcag acggtttccc ggcggctcgt tcatctggcc atacaccagg
     5221 gatactttgt cgataacgtt ggagtcggtc atttcgtggt agaagtcgtt accctcacga
     5281 gtacgttcac ctacgcccgc aaacacagag taaccggagt gctcgatcgc gatgttacga
     5341 atgagctcca tcatgtttac ggttttacct acacccgcac caccgaacag accaacttta
     5401 ccgcccttag cgaacggaca catcaggtcg ataactttga taccggtttc cagcagttcc
     5461 tgagagtttg acagctcttc gtaggaaggt gctgcgcggt gaatcgccca acgctcttct
     5521 tcaccgatct cgcctttcat gtcgaccggt tcacccagta cgttcatgat acggcccaga
     5581 gtcgctttac ctaccgggac ttcgatcggg tgttcgaggt cttttacatc cagacagcga
     5641 cgcagaccgt cggaggaacc cattgcgatg gtacgcacga taccgccgcc gagctgctgc
     5701 tgaacttcca gcaccagacg ctcattacca ttttgcacct caagagcatc gtacacgcgc
     5761 ggtacggcat cctgagggaa ttcgacgtca actacggcgc cgattacctg gacaatcttt
     5821 ccagtagcca tcttaaatcc tctacgaaat aacctgttta aaccgcggcg gcccccgaga
     5881 cgatctcggt gagttcctga gtaatgctgg cctgacgagc tttgttgtat accaactgca
     5941 gctctttaat caggctgccg ccattgtcgg tcgcggcttt catcgccacc atacgggcgg
     6001 cctgctcgct ggccaggttt tcaaccacgc cctgataaac ctgagattcg acataacgac
     6061 gcagcagggt atccagcaac gccttcggat cgggttcgta caggtaatcc caggatttat
     6121 gtttcagatc atcatcatct gatgccggta acggcagcag ctggctgatg gtcggaacct
     6181 gagacatggt gttaataaat ttgttgctga caatgtaaag cttgtccaga cggccttcgt
     6241 cgtaggcctg caacatcact tttaccggac cgatcagttc ggacagggaa gggttatccc
     6301 ccatgccggt gacctgggca acaacattgc cgcccacgga gttgaagaac gacacgcctt
     6361 tcgagccgat cattgcgagg tcgcattgaa cgcctttgtc ggtccaggtc ttcatttccg
     6421 ccagcagttt tttgaacagg ttaatgttca aaccaccgca caaaccacgg tcggtcgaca
     6481 ccaccaggta gcccacgcgt ttaacgtcgc ggtcttccag gtaagggtgc ttgtattcca
     6541 gattaccgtg tgcaaggtga ccaatcactt tgcgcatggt ttctgcataa ggacggctgg
     6601 ccgccatgcg atcctgcgat ttacgcattt tggaagcggc gaccatctcc atcgctttag
     6661 tgatcttttg cgtgttctgg acgcttgcga tcttactacg tatctctttt gcgccggcca
     6721 tgagcttctc ctcaatgcct tgcggcctgc cctaaggcaa gccgccagac gttaccagga
     6781 ttgggttgct ttgaaggaat cgaggatgcc tttcagcttg ccttcgattt cgtcgttgta
     6841 gccaccggtc tggttgatct cttgcatcaa cggagcgtga tcacggtcga cgtaagccag
     6901 cagagcggct tcgaagctgc cgattttcga cagttcaaca tccgccaggt aaccacgttc
     6961 tgctgcgaac agaaccagag actgttgcgc tacggacatc ggcgcatact gtttctgttt
     7021 cagcagttcg gtcactttct gaccgtggtc aagttgctta cgtgttgcat cgtcaaggtc
     7081 ggatgcaaac tgagagaacg ctgccagttc acgatactgt gccagagcgg tacggatacc
     7141 accggacagt tttttcatga tcttggtctg tgctgcacca ccaacacggg atacggaaat
     7201 acccgggtta accgcaggac gaataccggc gttgaacagg ttggtttcca ggaagatctg
     7261 accatcggta atggagatta cgttggtcgg aacgaacgca gaaacgtcac ccgcctgagt
     7321 ttcgataatc ggcagtgcgg tcagagaacc ggttttccct ttcacttcac ctttggtgaa
     7381 ggcttcaacg tattcggcgt taacacgcgc agcacgctcc agcagacgag agtggaggta
     7441 gaatacgtcg cccgggaatg cttcacgtcc tggcggacga cggagcagca gggagatctg
     7501 acggtaagca acagcctgtt tagacaggtc atcgtaaatg atcagcgcat cttcaccgcg
     7561 gtcacggaag tattcgccca ttgcgcaacc ggcatacggt gccaggtatt gcagtgcagc
     7621 ggattcagac gcggttgcta ccacaacgat ggtgttagcc agtgcgccgt gctcttccag
     7681 tttacgtacc acgttagaaa tggtggacgc tttctggccg atagcgacat agatacattt
     7741 gataccggaa tcgcgctggt tgatgatggc atcgatagcc agtgcggttt taccggtctg
     7801 acggtcaccg atgatcaatt cacgctgacc acgaccgatt gggatcatgg agtcaacggc
     7861 tttataaccg gtctgtaccg gctgatctac ggactgacgt tcgataacgc ccggagcgat
     7921 tgcttctaca gcagagaagc cgtcgtgatc cagcggacct ttaccgtcga ttggtgcacc
     7981 cagagtgtta accacacggc ccagcaggcc acggccaacc ggaacttcca ggatacggcc
     8041 agtacactta actttcatgc cttcggcaag gtcagcgtac ggacccataa caaccgcacc
     8101 tacagagtcg cgctcgaggt tcagtgcgat agcgtaacgg ttacccggca gggagatcat
     8161 ttcaccctgc atacaatcgg ccaggccgtg aatgcggata acaccgtcac ttacagaaac
     8221 aatagtacct tcgttgtgag cttcactcac aacattgaac tgagcaatgc gctgcttgat
     8281 cagttcgctg atttcggtgg aattcagttg catgctccag tccccttaag actgcaagac
     8341 gtctgcaagg cgctcaagac gaccgcgtac gctgccatca atgaccatat cacccgctcg
     8401 gatgataacg cctgccatta cagacttatc gattttgcaa ttcagcttaa ctttgcgtga
     8461 cagacgtttt tccatcgcag cagaaatttt cgcgagctgt tgttcactca gtgcggcagc
     8521 ggaaatgacg tctacctcag cggtagcctc actcacggca cgaaggtgaa taaactgctc
     8581 cagaacatcc gggagcgcgt taagacgacc attttcagcc attacccgaa taaggttctg
     8641 accgttttcg tccagttgct caccacaaac tgcgataaac gactcggcaa gcgtttctgg
     8701 cgcaagcgcg ccagagagaa gctctgccat ttgttcgttt ttggttacct cggcggcaaa
     8761 cgccagcatg tcctgccagc gttctacact ttggtgttcg acggcaaagt caaaagctgc
     8821 tttggcgtag gggcgagcta ccgtaataaa ttcagacatc agcccctccc tccttacagt
     8881 tcagcgacaa gtttatccac gatgtcgctg ttagcagctt catccacgga acgttcgatg
     8941 atcttctcgg cgccagcaac agccaggata gcaacttgct tacgcagctc ttcacgggca
     9001 cgtttacgct cggcttcaat ttccgcctgc gcctgggcca cgattttagt acgttcctgt
     9061 tctgcctcag ctttcgcttc gtccagaatc tgcgagcggc gtttgttcgc ctgctcgatg
     9121 attacctggg cttccgcttt cgcttttttc agctggtcgg tcgcgctggc ctttgcaagg
     9181 tcaaggtcct tatgtgctcg ttctgcggaa gcaaggccgt cagcaatttc tttttgacgt
     9241 ttttcgatgg ctgccattaa tggcggccat acgtacttca tgcagaacag aacgaacagg
     9301 acaaacgcga tggcctggcc gaggattgtt gcgttaagat tcacagcaca atgcctctat
     9361 ttagttaacg ttctgatatt gctctttaaa taaaagcaac gcttactacg cgacagcgaa
     9421 catcacgtac agacccagac ctacagcgat catcgggata gcatccacca gacccataac
     9481 gataaagaac tgagtacgca gcagaggaat cagatcaggt tgacgcgctg cgccttccag
     9541 gaatttaccc ccgaggatgc cgataccgat cgcagcaccg attgccgcca gacccatcat
     9601 cacagcggca gccatgtaca gcagatccat attcaggttt tccatgacag tctccagttt
     9661 gtttcagtta aaacgtagta gtgttggtaa attaatgttc ttcagacgcc atcgacagat
     9721 agacgatcgt cagaaccatg aagatgaagg cttgcagcgt aatgatcagg atgtggaaaa
     9781 tggcccacgg cacattcagg atccactgtg accaccacgg caacagacca gcaatcagaa
     9841 tgaaaatcag ctcaccggca tacatgttac cgaacagtcg caaaccgagt gaaactggtt
     9901 tggacagcag gcttacccct tcaaggatta agttgacagg aatgaacgcc cagtgattga
     9961 acggctgcag cgtcaactct ttcgtgaagc cgccgatgcc tttcattttg atgctgtaga
    10021 acagaatcag gataaatacg cccagtgcca tagacagcgt tacgttcacg tccgcagacg
    10081 gaaccacacg cagtgcaggc agacccagta catgttcagc aatgtacggc agcaggtcga
    10141 taggcagtaa atccatcagg ttcatcagga atacccagac gaagatcgtc agggccagcg
    10201 gagcaatcag cttgcttttg ccatggtaca tgtctttcac gctaccatta acaaagccga
    10261 tcaccagctc aatcgcggtc tgaaacttac ctggcacacc gctggtcgcc tttttggcta
    10321 cgctacggaa taaaaccagg aacaacagac ccagcaccac cgagaagaac atggagtcaa
    10381 tattgattgt ccagaaggtg gctggggggt tttgtggatc caccagcgag aatgtacgca
    10441 ggtccagctg aaggttattc aggtggtgtc ctatgtaatc ctgcggcgtc atattttctg
    10501 aagccatgat gccttttacc ctttgttgtt aattacagcc ggtgccagta tctgaaccac
    10561 cagcaccaaa acccacgtaa cgatcagcgg caagaatacc gcctttaaaa ccgccaacgc
    10621 caccaccagt aacaccaaca tcgccagaac tttgaaagct tcgccaaatg cgaatgtcca
    10681 ggccacccgg cctttcgctg gtgtatgcgc ctggtgacgc caggcaaata tcataaacaa
    10741 aacgttaggc agaaagactg ccaggccccc gcttattgca gagacgcccc agaaggggtc
    10801 tttgaggctg aacagcaatc cacttgctat caccaccagt aactgaacga gcagaagctt
    10861 ccgagcaacg tttcgactca cgagcgacac agacatcacg tttttcactc ctgctccctt
    10921 cgaggtatgc cgcgtgtcgt ataaaacttt ctttaaggct tagagtcaag catcaaaaag
    10981 cggtcaaatt atacggtgcg cccccgtgat ttcaaacaat aagtagccaa aaggtgaata
    11041 aatgtttaaa tatttttcca gtgcatacaa ttgcgacttt tctgctaacc ctgtttgatc
    11101 atgaaaaact gtaaataacg cgtaaacact ggtgataaag cgtgcttcag atcacatatt
    11161 gcgcatgttc gcgcacagca tatttattta cttggcaaat gatgcctttg caagtttatg
    11221 atatttcagt ctaaaaacag atactgtttt aataaatgac atttacacaa caaaaaccac
    11281 ccattgacat ttttaataat gttttaacag acaatgatgg ttcttagcgc cgatttttag
    11341 cagactgata ttttcactaa tgacttattt tctgcttacc aaaaaaagcc acgttatctt
    11401 gttgatgcaa aagagtgaac gtggcgttaa atgtaaccag ttatatcagt agaaaacctg
    11461 gttgttgtta acagtctaac cggtcaattt tttatgattt ttttgataaa aattaaattt
    11521 tatttgcttt aatcaccacc agatggcgtt cgccatccag ggcaggaacc tgaagtttaa
    11581 ccactgattc aacctgatat tcttcgggca acaaagcgat ttcatcttcc ggcatttgcc
    11641 ctttcagcgc gtagaaacgg ccttgctcac caggaagatg gtggcaccag ctcaccatat
    11701 cgttcagaga ggcaaaagcg cggctaatta cgccatcaaa tggcggctct gaaggaaact
    11761 cttctaccct gctctgtact ggctcaatat tctccagttt aagctcatgt tgcacctgac
    11821 gaaggaaacg cacgcgttta ccaaggctat ccaacagagt gaaatgggct tcaggacgca
    11881 cgatagagag tggaatgcca ggcagtccag gaccggtgcc gacatcgata aaccgttcac
    11941 cttgcagata cggtgccacc acaatgctat cgagaatatg gcgtaccagc atctcattag
    12001 gatcgcggac cgaagtcagg ttgtacgctt tgttccattt atgcagcata ttcacgtagg
    12061 caataagctg gtttttctgg tgatcggtaa gcgaaatacc tgcgtctttc agcagtaagg
    12121 agagtttgtt gagcacggtg attacctgtt cttgatgcgt tgcctggtaa gcgggtgctt
    12181 accaggcatt tttaatgcgt tatgcgctac gacgcagcat accctgtttt ttcagccaca
    12241 ccagcagaat ggagatggcc gcaggcgtga cgccagaaat acgtgaagcc tggccgatag
    12301 aggccggttt gtgatcgtta agtttggcga tcacttcgtt agaaagaccg gatacctggc
    12361 ggtaatccag tgtcgcgggt agcagggtgt tctcgttacg cagctgcttt tcgatctcat
    12421 cttgctggcg cgcgatataa ccttcgtatt taacctgaat ctcaacctgt tccgccgcct
    12481 gttcgtctgt caacgcaggg gcaaacggcg tcagcgtggt taatttttca taagtcattt
    12541 ccggacgacg cagcagatct tcaccactgg cttcacggga aagcggcgca gtcaggtgag
    12601 cattcacttc ggctgcagct tccgccgacg gggttaccca ggtcgatttc agacgctgac
    12661 gctcacgctc gatattctca agtttctcgt taaagcgcgc ccaacgttcg tcatccacca
    12721 ggcccagttc acgaccgatt tcagtcaaac gcagatccgc attatcttcg cgcagcatca
    12781 gacgatattc tgcgcgcgaa gtaaacatac gatacggttc tttggttcct aaagtgcaca
    12841 ggtcatcaac cagtacgccg agataggcct gagaacgcgc cggagcccaa ccttctttgt
    12901 cagcagacag acgggcagcg ttaagaccgg ccagcaaacc ttgcgcagcg gcttcttcgt
    12961 aaccggtagt gccgttaatc tgaccagcaa agaacagccc ctggataaac ttgctctcca
    13021 gcgtcggttt caggtcgcgt ggatcgaaga agtcatactc aatggcataa cccggacgca
    13081 cgatcttcgc gttttccatc ccctgcatgg agcggacgat ttgcatctgc acatcgaacg
    13141 gcaggctggt ggagataccg ttcggataaa tttcgttaga ggtcagccct tccggttcaa
    13201 ggaagatctg atgctgattt ctgtcggcga agcgcatgac tttgtcttcg atcgacgggc
    13261 agtagcgtgg gccgacacct tcgatcaccc ctgcgtacat tgggctacga tcgaggttac
    13321 tgcggatcac atcatgggtt ttctcgttgg tatgagtgat ataacacggc acctgctggg
    13381 gatgctggga cgcattgccc ataaacgaga ataccggcat tgggttatcg ccatgctgtt
    13441 gcgcaagcac gctaaagtcg atggttcgag catcaatacg cggtggtgtc ccggttttca
    13501 gacgaccaac gcgcagcgga agttcacgca aacggcgaga aagcggaatg gacggcggat
    13561 caccagcacg gccaccgctg tagttatcca gaccgatatg aattttaccg tcgaggaacg
    13621 tcccaacggt gagcacaacg gctttggcac ggaacttcag tcccatttgg gtaacagcac
    13681 cgaccacgcg atcgttttcg acaataagat cttcaaccgc ctgctggaag atcatcagat
    13741 tcggttggtt ctccagcgcc gtacgtaccg cctgacggta gagcacacga tccgcctgag
    13801 ctcgggtagc gcgaacagcc ggtcctttac ttgcgtttag tatcctaaac tggatacccg
    13861 cctgatcgat cgctttcgcc atcagaccgc cgagtgcatc cacttctttt accagatgtc
    13921 ccttcccaat accgccgatc gccgggttgc agctcatctg ccccagagtg tcgatattgt
    13981 gtgtcaaaag cagagtctgt tgacccatac gcgccgcagc catcgcggcc tcggtgcctg
    14041 catgaccccc gccaatgatg atgacgtcaa aaggatccgg ataaaacatg gtgattgcct
    14101 cgcataacgc ggtatgaaaa tggattgaag cccgggccgt ggattctact caactttgtt
    14161 ggcttgagaa agacctggga tcctgggtat taaaaagaag atctatttat ttagagatct
    14221 gttctattgt gatctcttat taggatcgca ctgccctgtg gataacaagg atccgacttt
    14281 taagatcaac aacctggaaa ggatcattaa ctgtgaatga tcggtgatcc tggaccgtat
    14341 aagctgggat cagaatgagg ggttatacac aactcaaaaa ctgaacaacc gttgttcttt
    14401 ggataactac cggttgatcc aagcttcctg acagagttat ccacagtaga tcgcacgatc
    14461 tgtatactta tttgagtaaa ttaatccatg atcccagcca ttcttctgcc ggatcttccg
    14521 gaatgtcgtg atcaagaatg ttgatcttca gtgtttcgcc cgtctgtttt gcaccggaat
    14581 ttttgagttc agcctcgagt ttatcgatag ccccacaaaa ggtgtcatat tcacgactac
    14641 caataccgat tgcgccaaag cggaccgcag aaagatcggg cttctgttcc tgcaatgctt
    14701 catagaaagg agaaaggttg tccggaatat ctccggcacc gtgggtggag ctgataacca
    14761 gccagatccc cgaggcagat aaatcttcca acagcggacc gtgcagcgtt tcggtggtaa
    14821 aacccgcctc ttccagcttt tcagccaggt gttctgctac atattcggca ccgccgaggg
    14881 tgctgccgct gataagagtg atatctgcca taaaccgcca cctttattaa gagtggcgta
    14941 ttgtacgctg tgaacgcgtt gggatctacc tgtggaaaag tatgggatta aaaaagccga
    15001 tcagggcttg atggtacgca tgatcgggtt ctgcaggacg atcagtgtct cggtggactg
    15061 aatttcatca attgtttgga tcttgttgat aagtacatgc tggagagcat cgatcgaacg
    15121 gcacatcact tttataaaga tgctgtagtg gccggtggtg tagtacgcct cggtcacttc
    15181 atcgaggctt tccagctttg ccagcgcgga aggatagtct ttggcgctct ttaatataat
    15241 gccgataaag cagcctacgt cataaccgag ctgcttcggg ctgacatcaa tacgcgcccc
    15301 ggtaatgatt cccgcctgct tcattttctc tactcgaacg tgaatcgtcc ccggactgac
    15361 gccaaattgt ttcgccagtt cggcgtaagc ggtgcgcgca ttgcccatta atgcttccag
    15421 gatgccacgg tccagattgt cgatcagata attttccata ggattttctt atgcggattg
    15481 atgattcatt ctattttagc cttctttttt aatgaatcaa aagtgagtta ggctttttat
    15541 tgaatgatta ttgcatgtgt atcggttttt gttgcttaat cataagcaac aggacgcagg
    15601 agtataaaaa atgaaaaccg cttacattgc caaacaacgc caaattagct tcgtgaaatc
    15661 tcacttttct cgccaactgg aagaacgtct ggggctgatc gaagtccagg cgccgattct
    15721 tagccgtgtg ggggatggca cgcaggataa cttgtcgggc tgtgaaaaag cggtgcaggt
    15781 aaaagtgaaa gctctgcctg atgcccagtt cgaagtggtt cattcactgg cgaagtggaa
    15841 acgtcagacc ttagggcaac acgacttcag cgcgggcgaa gggctgtaca cgcacatgaa
    15901 agcccttcgc cccgatgaag accgtctttc tccgttgcac tcggtctatg ttgaccagtg
    15961 ggactgggaa cgcgtaatgg gcgacggtga gcgtcaattc tcgactctga aaagcacggt
    16021 agaggcgatc tgggcgggaa ttaaagcaac cgaagctgcg gttagcgaag agtttggcct
    16081 ggcaccgttc ctgccggatc agatccactt cgtacacagc caggagttac tgtctcgtta
    16141 tccggatctt gatgccaaag ggcgtgagcg ggcgatagcg aaagatcttg gtgcggtatt
    16201 ccttgtcggg attggcggca agctgagcga tggtcatcgt cacgacgtgc gcgcaccgga
    16261 ttatgatgac tggagcaccc cgtcagagct gggccatgcg ggcctgaacg gcgatattct
    16321 ggtgtggaac ccggtactgg aagatgcgtt tgagctttct tccatgggga tccgcgtgga
    16381 tgccgacacg ttgaagcatc agttggcgct gaccggtgat gaagatcgtc tgcagctgga
    16441 atggcatcag gcgttgctgc gcggtgaaat gccgcagacc atcggcggcg gtatcggcca
    16501 gtctcgtttg accatgctgc tgctgcaact gccgcatatc ggccaggttc agtgtggagt
    16561 atggccagct gctgttcgcg agagcgtccc ttctctgctg taataattta tcgccgccag
    16621 cgtctgagca ggcggcttcg catcccggta tcaaagcgcc agatatgatc gaaaatgcgc
    16681 atgatgccgg gtttgccgtg tgccgacatc gccacggcat gaaagcgatg ctgatgtacc
    16741 cgctgcagct ctttcacttt actcgtcacg tcgtcaggca accgctgggc gataaaatca
    16801 gaaatcacca ccgcatcggc atcaaaccat tccttgcttt gcaagcgttc cataatggcg
    16861 cgaaaacaac tggcaagatc ggtgccgcca cgaaactgct ggcttaaaaa acggattgct
    16921 tgttcgatgc cttgtgggcc tgaaagctca taacggacga tctcggtgga aaatagcata
    16981 atataacagc gccggttttc agcgagagca atgcgcatca aagccaggca gaacgctttc
    17041 gcacactgtt cattaaagcc gcccattgag ccggaagtat ccacgcagac aataaacggt
    17101 ccgcgcggct gttcgtcgta atctttatgc accaccgggc gttcgatcac tttttcacgc
    17161 cacgactcac cgtgcaggcg ataggtgagc aactgttttt ccaccagccg acggtaaaac
    17221 tcatactcca gttccgttat ccctagtgtc gccagttctg gcggcagaag acgtaaaata
    17281 tcatcgcttt gttgcagacc atcaacctgc tcaggaacag ttgccggttc gcgcaccatg
    17341 gtgcggaagg tttccatctg cgcatcgttg cgcggtattg atttggcttc ccgggaacgc
    17401 cccagctgtt ctgccaggcg tttcagttcc ggctgttcgt taagaaattc accgtatttc
    17461 acaatcaact gatagtcgcc acgtttaagc tgaccggcgc tcatatccca cagacgacca
    17521 gctgcggtat tgttatctgc gagaatcggt tcaagttgtc cgctcagcgt catgcgttcc
    17581 tgaacttcac tcaacagttg ttcgcgttct tcttctaata actgttgatt taacgtcgtt
    17641 gcttgcacga tcagacttaa acgccaacgc tggaggaaaa gcgtgtgtaa cgctgaagtg
    17701 atcgtgctgt tagcatcaac caactgtcgg gcttgttctg cccatgggga attcagacga
    17761 tgcagtaagt ccaggatctg tggtagctgc acaataaact gcggcgtgga gaggagctgg
    17821 ctttgctgat agcacatcac ctcttcggtg agttccggcg ggactcgggc atctttcagc
    17881 cgattacgca gcgcctcacg ccagcgggga acatcatcag tgattgccgc cttcagtcgt
    17941 gggaattttt caaagaagac cgctagctgc ggtgaggcca gcagcgcgat gatcatctct
    18001 tcgatcaatc cctcttcgct gacggccagc atcacattaa gcgtatccag cgttagcatt
    18061 gttgtgcctg gcgaatctgt gcgccgacat cctgtaggct ggcttcaatg cgacctaacc
    18121 agtcgccagg gataaacaga catttttgct gttcgctgaa taaagcgtgt tgcttacgcc
    18181 agtcgctttc aagctcttcc agttgttgtt ttatttcacc tggcagacct tcagccgatg
    18241 aaccgggaag agccagcgta ctgccttgta aacttacatc gcgtacaaca agatgttggg
    18301 tgctatcaac ttccagattc agtttctggg caaagccgat accgttcagt ttgccgcgaa
    18361 tttcaccacc tttactcagc cactgttcca gcgcgctacg ctcaaaggag atatgaacca
    18421 cttccatatc atgcagtttt aacggttttt gcagcagcag agtcagagtg gaagcagtaa
    18481 cgttaacagg gagttgatac tgctgacgac ggctgaaaat gccgcccaga cgaattaccg
    18541 ttaaggctgt tttatcactt tgttgctgct ggagttgcag gtgacgttgc acaatcgcgc
    18601 caaggcgagt caacatccct tgctgttgcc aggcgtgacc ggtcatcaat acatcaattt
    18661 gttgttgtat caaattcagg ctttgcgcgt cataccacag gcaatctttc agcaaaatga
    18721 gatctaccgg ggcaacagca ctgcgaccgc taaaaaaggc gcaggcctgc aataagcgga
    18781 tcgctttttt ccagcgacga tccgagacat aaggcgcatc cggtaattta tccagttgct
    18841 ggcgcagcat aaaaatcagc tcaaatacat gatcgggcag cgtaatttca ccaatctctt
    18901 tctgccagcg ttcatattct tcatctgtga cctgcaaggc atcaggaacc ggattgtcgt
    18961 tttcatcctg ttgactggtc agcatggagc ggaaattcgc tttatcctgc actttatcta
    19021 accacagacg aatcagcatg cggtcatata acgcttccag actgctgtct gcttccggca
    19081 gctcgttgga ggccgccacc agtagacgca tcgggatttt ttctacgtgt gcaccgttgc
    19141 ggaactggcg ctcgttaatg gcggtgagca aggtattaag aattgccggg cccgctttcc
    19201 agatctcatc cagaaagaca atttctgctt ccggcaggta accgctggtt aaacgttcat
    19261 agcgcccttc atcttttagc gcctgaatag aaaggggacc aaaaacttct tccggcgtgg
    19321 agaagcgagt catcagatat tcaaacgcgc gggcattctg aaaggcgaat tttaagcgcc
    19381 gggcgatcaa acttttggca atacctggcg ggccaaggag gaacacactt tcaccactta
    19441 atgccgctaa taaacacaag cggatggcgt ggctacgttc ataaagcccc ttttccaacg
    19501 aactgctcag gcgggaaatt ctttccgcta ataaatgagg gtgagccata atgaagtggc
    19561 gtcctttcgt caaaagttct gcgtaaattg cgagtataga cgtttcttgc tggtggctaa
    19621 aatagtctca aagggggggg atttttcttt gagccaggtt aatgtggccg catttaggag
    19681 tacgattttg ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca
    19741 cacatttcat atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc
    19801 cgcgattacc ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata
    19861 tacacttcgt gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt
    19921 tggcttttta tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac
    19981 cttcgtgatg cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc
    20041 cgggcgtaat acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg
    20101 cagttttttc tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga
    20161 aggtctggaa atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt
    20221 tctcacttta ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc
    20281 gccgatcatg ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc
    20341 taacccggaa gtgctgcatg cgctgaatcc gatgtgggcg gtgcatttct tccttgaata
    20401 caaaacggtt tcttttattg cattaggggc agtggtgctg tcgattacgg gggtcgaggc
    20461 gctgtatgct gatatgggac actttggtaa gttccctatt cgtctggcgt ggttcactgt
    20521 cgtattacct tccttaactc ttaattactt cggccaggga gcgctgttgt taaagaaccc
    20581 ggaagcgatt aagaacccgt tcttcctttt ggcaccggac tgggcgctga tcccgctgct
    20641 gatcatcgcc gcactggcga cggtaatcgc ctcgcaggcg gttatctctg gcgtcttttc
    20701 attgacgcgt caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc
    20761 cgaaatggag tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt
    20821 cgtgattgtg attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc
    20881 ggtgaccgga accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg
    20941 gcactggaat aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc
    21001 attgttcacc gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg
    21061 tactgtgatg tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg
    21121 gatgcatgaa catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc
    21181 tgttcgcgtg cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc
    21241 gctgatgcat aacctcaaac ataacaaggt attgcatgag cgggtgattc tgttaactct
    21301 gcgcaccgaa gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc
    21361 gcccactttc tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga
    21421 agttttccac cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt
    21481 tatgtcgcat gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa
    21541 gctgtacttg ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc
    21601 aaacagggtt atcgaactgg gtactcaggt cgaaatctaa cgccagacgc ctcctttctt
    21661 cataaggggg cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc
    21721 acatttccgt aacgtcacga tggttttccc aactcagtca ggattaaact gcgggtcagc
    21781 gaaacgtttc gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt
    21841 catcggtgat ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctgggttac
    21901 ccatccccaa aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta
    21961 tgcaggtgct gggcgtcgtc acaaatgaaa tgcaggtcga ggcggccatt atcgcggaag
    22021 agatcaaaca acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc
    22081 aaaaacacca gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac
    22141 aaaccgcaga aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca
    22201 ttctctgtgc tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc
    22261 atcgataaag ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg
    22321 ggccgcgtga tggcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg
    22381 cttactggta tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca
    22441 tttaccgggc cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac
    22501 ctgatcccgc agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc
    22561 tttggcaaaa ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt
    22621 aacctgcgct ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg
    22681 gttgaaatcg ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc
    22741 gatgcgctga ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg
    22801 caaggccgcg gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat
    22861 gacgttaccg tttttcgtga cggacaattt attgctgagc gcgaagtggc atcactgacc
    22921 gaagattcgc tgattgagat gatggtgggt cgcaagctgg aagatcaata tccgcacctg
    22981 gacaaagcgc cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac
    23041 gatgtctctt ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca
    23101 ggtcgtaccg aactgatgaa agtgctctac ggcgcactac cgcgcaccag cggttacgtc
    23161 accctggatg ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt
    23221 gtgtatatct ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag
    23281 aacatgtcgc tgacagcgct gcgctacttc agccgcgctg gtggcagttt gaagcatgcc
    23341 gatgaacagc aggctgtgag tgatttcatt cgtctgttta atgtgaaaac tccgtcgatg
    23401 gaacaggcaa ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt
    23461 ctgatgacac gccccaaggt gttgatcctc gatgagccta cccgtggcgt agatgtcggc
    23521 gcgaaaaaag agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt
    23581 ctggtgtcat cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat
    23641 gaagggcatc tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct
    23701 gccgctgtgg gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt
    23761 ctctggtcgc cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct
    23821 gctggtgctg atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt
    23881 attcaatatt ctccagcaaa cttcggtgaa cgccattatg gcggttggga tgacgctggt
    23941 gatcctgacg tcgggcatag acttgtcggt aggttctctg ttggcgctaa ccggcgcagt
    24001 tgctgcatct atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ccgctctcgc
    24061 gttaggtgcc gcaattggtg cggtaaccgg agtgattgta gcgaaaggtc gcgtccaggc
    24121 gtttatcgct acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa
    24181 cggtagccca gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat
    24241 tggtcgtccg ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc
    24301 ctggtacatg ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga
    24361 agcggcaacg cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct
    24421 ttgtggtctg ctggcatcac tggccgggat cattgaagtg gcgcgtctct cctccgcaca
    24481 acccacggcg gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac
    24541 gagtctggcg ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg
    24601 cttccttaat aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa
    24661 agcggtggtg attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca
    24721 ggacatcttg aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag
    24781 cgccaccgtc agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct
    24841 taacaacccg ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataaacttgg
    24901 ctataacctg gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca
    24961 ggacttaacc gttcgcggca caaaaattct gctgattaac ccgaccgact ccgacgcagt
    25021 gggtaatgct gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca
    25081 ggcaacgaaa ggtgaagtgg tgagccacat tgcatctgat aacgtactgg gcggcaaaat
    25141 cgccggtgat tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg
    25201 cattgctggt acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgtagctgc
    25261 tcacaagttt aatgttcttg ccagccagcc agcagatttt gatcgcacca aaggtttgaa
    25321 cgtaatgcag aacctgttga ccgctcatcc ggatgttcag gctgtattcg cgcagaatga
    25381 tgaaatggcg ctgggcgcgc tgcgcgcact gcaaactgcc ggtaaatcgg atgtgatggt
    25441 cgtcggattt gacggtacac cggatggcga aaaagcggtg aatgatggca aactagcagc
    25501 gactatcgct cagctacccg atcagattgg cgcgaaaggc gtcgaaaccg cagataaagt
    25561 gctgaaaggc gagaaagttc aggctaagta tccggttgat ctgaaactgg ttgttaagca
    25621 gtagttttaa tcaggttgta tgacctgatg gtgacataaa tacgtcatcg acagatgaac
    25681 gtgtaatata aagaaaagca gggcacgcgc caccctaaca cggtggcgca ttttatggac
    25741 atcccgaata tgcaaaacgc aggcagcctc gttgttcttg gcagcattaa tgctgaccac
    25801 attcttaatc ttcaatcttt tcctactcca ggcgaaaccg taaccggtaa ccactatcag
    25861 gttgcatttg gcggcaaagg cgcgaatcag gctgtggctg ctgggcgtag cggtgcgaat
    25921 atctccttta ttgcctgtac tggcgatgac agcattggcg agagcgttcg ccagcagctc
    25981 gccactgata acatcgatat tactccggtc agcgtgatca aaggcgaatc aacaggtgtg
    26041 gcgctgattt ttgttaatgg cgaaggtgag aatgtcattg gtattcatgc cggcgctaat
    26101 gctgcccttt ccccggcgct ggtggaagcg caacgtgagc gtattgccaa cgcgtcagca
    26161 ttattaatgc agctggagtc accactcaaa agtgtgatgg cagcggcgaa aatcgcccat
    26221 caaaataaga ctatcgttgc gcttaacccg gctccggctc gcgaacttcc tgacgaactg
    26281 ctggcgctgg tggacattat tacgccaaac gaaacggaag cagaaaagct caccggtatt
    26341 cgtgttgaaa atgatgaaga tgcagcgaag gcggcgcagg tactgcatga aaaaggtatc
    26401 cgtactgtac tgattacttt aggaagtcgt ggtgtatggg ctagcgtgaa tggtgaaggt
    26461 cagcgcgttc ctggattccg ggtgcaggct gtcgatacca ttgctgccgg agataccttt
    26521 aacggcgcgt taatcacggc gttgctggaa gaaaaaccat tgccagaggc gattcggttt
    26581 gcccatgctg ccgctgcgat tgccgtaaca cgtaaaggcg cacaaccttc cgtaccgtgg
    26641 cgtgaagaga tcgacgcatt tttagacagg cagaggtgac gcttggctac aatgaaagat
    26701 gttgcccgcc tggcgggcgt ttctacctca acagtttctc acgttatcaa taaagatcgc
    26761 ttcgtcagtg aagcgattac cgccaaagtt gaagcggcga ttaaagaact caattacgcg
    26821 ccatcagctc tggcgcgtag cctcaaactc aatcaaacac ataccattgg catgttgatc
    26881 actgccagta ccaatccttt ctattcagaa ctggtgcgtg gcgttgaacg cagctgcttc
    26941 gaacgcggtt atagtctcgt cctttgcaat accgaaggcg atgaacagcg gatgaatcgc
    27001 aatctggaaa cgctgatgca aaaacgcgtt gatggcttac tgttactgtg caccgaaacg
    27061 catcagcctt cgcgtgaaat catgcaacgt tatccgacag tgcctaccgt gatgatggac
    27121 tgggctccgt
//
LOCUS       DAZFKF010000065        26973 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFKF010000065 DAZFKF010000000
VERSION     DAZFKF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.065"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            247..2898
                     /gene="ppc"
                     /locus_tag="ACHKA1_003225"
     CDS             247..2898
                     /gene="ppc"
                     /locus_tag="ACHKA1_003225"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO5680836.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            3081..4814
                     /gene="eptC"
                     /locus_tag="ACHKA1_003226"
     CDS             3081..4814
                     /gene="eptC"
                     /locus_tag="ACHKA1_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO5680837.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYIVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            5029..5880
                     /gene="yijO"
                     /locus_tag="ACHKA1_003227"
     CDS             5029..5880
                     /gene="yijO"
                     /locus_tag="ACHKA1_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5680838.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTAKLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(5867..6208)
                     /gene="frwD"
                     /locus_tag="ACHKA1_003228"
     CDS             complement(5867..6208)
                     /gene="frwD"
                     /locus_tag="ACHKA1_003228"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO5680839.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGISIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(6210..7088)
                     /gene="pflC"
                     /locus_tag="ACHKA1_003229"
     CDS             complement(6210..7088)
                     /gene="pflC"
                     /locus_tag="ACHKA1_003229"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO5680840.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(7054..9351)
                     /gene="pflD"
                     /locus_tag="ACHKA1_003230"
     CDS             complement(7054..9351)
                     /gene="pflD"
                     /locus_tag="ACHKA1_003230"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO5680841.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAAGCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(9402..9722)
                     /gene="frwB"
                     /locus_tag="ACHKA1_003231"
     CDS             complement(9402..9722)
                     /gene="frwB"
                     /locus_tag="ACHKA1_003231"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO5680842.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(9737..10816)
                     /gene="frwC"
                     /locus_tag="ACHKA1_003232"
     CDS             complement(9737..10816)
                     /gene="frwC"
                     /locus_tag="ACHKA1_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO5680843.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAICVNVL
                     KSLARKKGSSTDEKEDDLDLDFEIN"
     gene            11125..13626
                     /gene="ptsP"
                     /locus_tag="ACHKA1_003233"
     CDS             11125..13626
                     /gene="ptsP"
                     /locus_tag="ACHKA1_003233"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5680844.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            13638..14300
                     /gene="fsa"
                     /locus_tag="ACHKA1_003234"
     CDS             13638..14300
                     /gene="fsa"
                     /locus_tag="ACHKA1_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO5680845.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDKGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            14311..15414
                     /gene="gldA"
                     /locus_tag="ACHKA1_003235"
     CDS             14311..15414
                     /gene="gldA"
                     /locus_tag="ACHKA1_003235"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO5680846.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            15688..16305
                     /gene="yijF"
                     /locus_tag="ACHKA1_003236"
     CDS             15688..16305
                     /gene="yijF"
                     /locus_tag="ACHKA1_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO5680847.1"
                     /translation="MKASLVLVSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIEDGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(16332..17237)
                     /gene="yijE"
                     /locus_tag="ACHKA1_003237"
     CDS             complement(16332..17237)
                     /gene="yijE"
                     /locus_tag="ACHKA1_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO5680848.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(17331..19511)
                     /gene="katG"
                     /locus_tag="ACHKA1_003238"
     CDS             complement(17331..19511)
                     /gene="katG"
                     /locus_tag="ACHKA1_003238"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO5680849.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(19840..20730)
                     /gene="metF"
                     /locus_tag="ACHKA1_003239"
     CDS             complement(19840..20730)
                     /gene="metF"
                     /locus_tag="ACHKA1_003239"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO5680850.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            20806..21051
                     /locus_tag="ACHKA1_003240"
     CDS             20806..21051
                     /locus_tag="ACHKA1_003240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680851.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     LTNHVLTASCRIRRKRFIRPNPMIIQYISLETIDPKT"
     gene            complement(21079..23511)
                     /gene="metL"
                     /locus_tag="ACHKA1_003241"
     CDS             complement(21079..23511)
                     /gene="metL"
                     /locus_tag="ACHKA1_003241"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO5680852.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYDIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(23514..24674)
                     /gene="metB"
                     /locus_tag="ACHKA1_003242"
     CDS             complement(23514..24674)
                     /gene="metB"
                     /locus_tag="ACHKA1_003242"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO5680853.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            24951..25268
                     /gene="metJ"
                     /locus_tag="ACHKA1_003243"
     CDS             24951..25268
                     /gene="metJ"
                     /locus_tag="ACHKA1_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO5680854.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            25328..25936
                     /gene="yiiX"
                     /locus_tag="ACHKA1_003244"
     CDS             25328..25936
                     /gene="yiiX"
                     /locus_tag="ACHKA1_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO5680855.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSSPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            26081..26756
                     /locus_tag="ACHKA1_003245"
                     /pseudo
     CDS             26081..26756
                     /locus_tag="ACHKA1_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1H family transposase"
     gene            complement(26843..>26973)
                     /locus_tag="ACHKA1_003246"
     CDS             complement(26843..>26973)
                     /locus_tag="ACHKA1_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFO5680856.1"
                     /translation="VFFYESPTSTHNDIMGGGGMSYAYEYDVKYNICHLRKKSRMR"
BASE COUNT         6544 a         7482 c         6872 g         6075 t
ORIGIN      
        1 ctttggtgtt acttgaggcg attttttaac attcccataa gttacgctta tttaaagcgt
       61 cgtgaattta atgacgtaaa ttcctgctat ttattcgttt gctgaagcga tttcgcagca
      121 tttgacgtca ccgcttttac gtggctttat aaaagacgac gaaaagcaaa gcccgagcat
      181 attcgcgcca atgcgacgtg aaggatacag ggctatcaaa cgataagatg gggtgtctgg
      241 ggtaatatga acgaacaata ttccgcattg cgtagtaatg tcagtatgct cggcaaagtg
      301 ctgggagaaa ccatcaagga tgcgttggga gaacacattc ttgaacgcgt agaaactatc
      361 cgtaagttgt ccaaatcttc acgcgctggc aatgatgcta accgccagga gttgctcacc
      421 accttacaaa atttgtcgaa cgacgagctg ctgcccgttg cgcgtgcgtt tagtcagttc
      481 ctgaacctgg ccaacaccgc cgagcaatac cacagcattt cgccgaaagg cgaagctgcc
      541 agcaacccgg aagtgatcgc ccgcaccctg cgtaaactga aaaaccagcc ggaactgagc
      601 gaagacacca tcaaaaaagc agtggaatcg ctgtcgctgg aactggtcct cacggctcac
      661 ccaaccgaaa ttacccgtcg tacactgatc cacaaaatgg tggaagtgaa cgcctgttta
      721 aaacagctcg ataacaaaga tatcgctgac tacgaacaca accagctgat gcgccgcctg
      781 cgccagttga tcgcccagtc atggcatacc gatgaaatcc gtaagctgcg tccaagcccg
      841 gtagatgaag ccaaatgggg ctttgccgta gtagaaaaca gcctgtggca aggcgtaccg
      901 aattacctgc gcgaactgaa cgaacaactg gaagagaacc tcggctacaa actgcccgtc
      961 gaatttgttc cggtacgttt tacctcgtgg atgggcggtg accgcgacgg caacccaaac
     1021 gtcactgccg atatcacccg ccacgtcctg ctgctcagcc gttggaaagc caccgatttg
     1081 ttcctgaaag atattcaggt gctggtttct gaactgtcga tggttgaagc gacccctgaa
     1141 ctgctggcgc tggttggcga agaaggtgcc gcagaaccgt atcgctatct gatgaaaaac
     1201 ctgcgttctc gcctgatggc gacacaggca tggctggaag cgcgcctgaa aggcgaagaa
     1261 ctgccaaaac cagaaggcct gctgacacaa aacgaagaac tgtgggaacc gctctacgct
     1321 tgctaccagt cacttcaggc gtgtggcatg ggtattatcg ccaacggcga tctgctcgac
     1381 accctgcgcc gcgtgaaatg tttcggcgta ccgctggtcc gtattgatat ccgtcaggag
     1441 agcacccgcc ataccgaagc gctgggcgag ctgacccgct acctcggtat cggcgactac
     1501 gaaagctggt cagaggccga caaacaggcg ttcctgatcc gcgaactgaa ctccaaacgt
     1561 ccgcttctgc cgcgcaactg gcaaccaagc gccgaaacgc gcgaagtgct cgatacctgc
     1621 caggtgattg ccgaagcgcc gcaaggctcc attgccgcct acgtgatctc gatggcgaaa
     1681 acgccgtccg acgtgctggc tgtccacctg ctgctgaaag aagcgggtat cgggtttgca
     1741 atgccggttg ctccgctgtt tgaaaccctc gatgacctga acaacgccaa cgatgtcatg
     1801 acccagctgc tgaatatcga ctggtatcgc ggcctgattc agggcaaaca gatggtgatg
     1861 attggctatt ccgactcagc aaaagatgcg ggcgtgatgg cagcttcctg ggcgcaatat
     1921 caggcacagg atgcattaat caaaacctgc gaaaaagcgg gtattgagct gacgttgttc
     1981 cacggtcgcg gcggttccat tggtcgcggc ggcgcacctg ctcatgcggc gctgctgtca
     2041 caaccgccag gaagcctgaa aggtggcctg cgcgtgactg aacagggcga gatgatccgc
     2101 tttaaatatg gtctgccaga aatcaccgtc agcagcctgt cgctttacac cggggcgatt
     2161 ctggaagcca acctgctgcc accacctgag ccgaaagaga gctggcgtcg cattatggat
     2221 gaactatcag tcatctcctg cgatctctac cgtggctacg tacgtgaaaa caaagatttt
     2281 gtgccttact tccgctccgc tacaccggaa caagaactgg gcaaactgcc gttgggttca
     2341 cgtccggcga aacgtcgccc gaccggcggc gtcgagtcgc tgcgcgccat tccgtggatt
     2401 tttgcctgga cgcaaaaccg tctgatgctc cccgcctggc tgggtgcagg tacagcgctg
     2461 caaaaagtgg tcgaagatgg caaacagagc gagctggagg ctatgtgccg cgattggcca
     2521 ttcttctcga cgcgtctcgg catgctggaa atggtattcg ccaaagcaga tctgtggctg
     2581 gcggaatact atgaccaacg cctggtagac aaagcactgt ggccgttagg taaagagtta
     2641 cgcaatctgc aagaagaaga catcaaagtg gtgctggcga ttgcgaacga ttcccatctg
     2701 atggccgatc tgccgtggat tgcagagtct attcagctac ggaatattta caccgacccg
     2761 ctgaacgtat tgcaagccga gttgctgcac cgctcccgcc aggcagaaaa agaaggccag
     2821 gaaccggatc ctcgcgtcga gcaggcgtta atggtcacta ttgccgggat tgcggcaggt
     2881 atgcgtaata ccggctaatc ttcctctcct gcaaaccctc gtgcttttgc gcgagggttt
     2941 tctgaaatac ttctgttcta acaccctcgt tttcaatata tttctgtctg tattttattc
     3001 aaattctgga tatcccttca gatatcctta aggaattgtc ggtacattcg gcgatatttt
     3061 tttcaagaca ggttcttact atgcattcca cagaagtcca ggctaaacct ctttttagct
     3121 ggaaagccct gggttgggca ctgctctact tttggttttt ctctactctg ctacaggcca
     3181 ttatttacat cagtggttat agtggcacca acggcattcg cgactcgctg ttattcagct
     3241 cgctgtggtt aattccggta ttcctctttc cgaagcggat caaaattatt gccgcagtga
     3301 tcggcgtggt gctatgggcg gcctctctgg cggcgctgtg ctactacgtc atctacgggc
     3361 aggagttctc gcagagcgtt ctgtttgtga tgttcgaaac caacaccaac gaagccagcg
     3421 agtatttaag ccagtatttc agcctgaaaa ttgtgcttat cgcgctggcc tatacggcag
     3481 tggcagttct gctgtggaca cgtctgcgcc cggtctatat tccaaagccg tggcgttata
     3541 ttgtctcttt tgccctgctt tatggcttga ttctgcatcc gatcgccatg aatacgttta
     3601 tcaaaaacaa gccgtttgag aaaacgttgg ataacctggc ctcgcgtatg gagcctgccg
     3661 caccgtggca attcctgacc ggctattatc agtatcgtca gcaactaaac tcgctaacaa
     3721 agctactgaa tgaaaataat gccttgccgc cgctggctaa tttcaaagat gaatcgggta
     3781 acgaaccgcg caccttagtg ctggtgattg gcgagtcaac ccagcgtgga cgcatgagtc
     3841 tgtacggtta tccgcgtgaa accacgccgg agctggatgc gctgcataaa accgatccga
     3901 atctaaccgt gtttaataac gtagttacgt ctcgtccgta caccattgaa atcctgcaac
     3961 aggcgctgac ctttgccaat gaaaagaacc ctgatctgta tctgacgcag ccgtcgctga
     4021 tgaacatgat gaaacaggcg ggttataaaa ccttctggat caccaaccag cagacgatga
     4081 ccgcccgcaa taccatgctg acggtatttt cgcgccagac cgacaagcag tactacatga
     4141 accagcaacg tacgcagagt gcgcgtgaat acgacactaa cgtgctgaag ccgttccagg
     4201 aagtgctgaa ggaccctgcg ccgaagaaac tgatcattgt tcatctgctg ggtacgcata
     4261 tcaaatacaa ataccgctac ccggaaaatc agggcaagtt tgatggcaat accgatcatg
     4321 ttccaccggg attaagcgca gaagaactgg agtcatataa cgattatgac aacgctaacc
     4381 tgtataacga tcatgtggtt gccagcctga ttaaagactt taaagcggca gacccgaacg
     4441 gattcctggt ttacttctct gaccacggtg aagaggttta cgacacaccg cctcataaaa
     4501 cccaggggcg taatgaggac aacccgacgc gtcatatgta caccattccg ttcctgctgt
     4561 ggacgtcaga aaaatggcaa gcgactcatc cgcgtgattt ctcgcaggat gttgatcgta
     4621 aatacagcct tgcggaactg atccacacct ggtcagattt agcaggctta tcttacgacg
     4681 gttacgatcc aacccgttca gtggtgaatc cgcagttcaa agaaactacc cgctggattg
     4741 gtaacccgta caagaaaaac gcgctgatcg attacgacac gctgccatat ggcgaccagg
     4801 taggtaatca gtaattatca gccaggcgct atttgccgga tgcgacgctt gacgcgtctt
     4861 atccggccta cagatcgcgg cggtagactg gataaggcgt ttacgccaca tccggcaata
     4921 caactgccgc aatgtttaca tcgaactccc ctcaggccac acttttcagg tggccttttt
     4981 taataatgcc agttactgtt ttcccgtcta catacctaag ggaataacat gtatcacgac
     5041 gtcagctatc tgctctcccg cctgatcaac ggcccgctgt ccctgcgcca gatttacttt
     5101 gccagcagta acggcccggt gcccgatctt gcgtatcagg tcgattttcc acggctggaa
     5161 attgtgctgg aaggtgagtt tgttgatacc ggcgctggag caacgttagt tcccggcgat
     5221 gtgctgtacg ttcctgctgg tggctggaat tttccacaat ggcaagcccc cgccactacc
     5281 tttagcgtgc tgtttggcaa acagcaactc ggcttcagcg tcgtgcaatg ggatggcaaa
     5341 caatatcaaa atctggcgaa gcaacacgtc gcccgacgcg gcccacgcat aggttctttt
     5401 ctgctacaaa cgctcaatga aatgcaaatg cagccgcagg agcagcaaac ggcaaagctt
     5461 atcgtcgcca gcctgcttag ccactgccgc gatttgctcg gcagccaaat ccagaccgcc
     5521 tcacgcagcc aggcactgtt cgaagctatt cgtgattata tcgacgaacg ctatgcctcc
     5581 gcgcttaccc gcgaatctgt cgcacaggca ttctatattt caccaaacta tctctcgcac
     5641 ctgtttcaaa aaacgggggc cattggtttt aacgaatacc tgaatcacac gcgactggag
     5701 cacgctaaga cgttattgaa aggctatgac ctgaaggtaa aagaagtggc gcacgcctgc
     5761 ggctttgtcg acagcaacta cttctgccgt ctgtttcgta aaaacaccga acgctcgccg
     5821 tcggaatatc gtcggcagta ccacagccaa ctgacagaaa aaccgactac tccagaataa
     5881 gatgggtttg ctgcggcgca gaaagcactt tgcgcaccgc gctcattacc cgctgcggct
     5941 cacgcaggaa tgcgtagatg ctgcattgca catagcggca atgttcaaat cgctcggcac
     6001 cggcaagctc gatatccgta atcaacagag caacatccgc ccgacggata tcctcgtccg
     6061 ctaaacgatt ctccgttccc agcgcgccct gagtttcaat gctgatgccc cacttctcta
     6121 actggcacaa cttttccagc cgttccgccg ccatataagt atgcgccacg ccgcttacgc
     6181 aggcggttac tgccaccagg tatgccattt taacctccca cggtaacctg aaatccggcc
     6241 cgttctgcca tttcgcgcat cgttgccaca tcagctgacg acggcgcagg cacctctttc
     6301 atcgaccatg ttttccccag caggcggtat ttcggttcgc cgtactgatg aaacggtaac
     6361 agatggatct gcctgatatt cagcgggatc agcacgtcca gcgcctgctg catattctcc
     6421 cggctgagcg tgaaaccagg gatcagcggt aaacgcggga tcacgttgac gccctcactc
     6481 accagcaaac gcagattctc cagcacgcgt ggcaggttca tcttcaccac atcccgcgcc
     6541 tgagtcgcgt ccataatttt taaatcgaac aacacttcat cgcacaattt cgccagtggt
     6601 aacagcttgc tggctggtgc gtctccggca gtttcaatgg cgcatgacac accccacagc
     6661 cgcagtcgct gtaaaaaacg ggtagcaaac tccgcctgca ttaacacttc gccgccagaa
     6721 agcgtgacgc cgccgccgga cgtgcgaaag aaaatgtcat ctttcatcac ttcccgttcc
     6781 agcgcgtcaa ggctgatatc gcgaccaatc cgttcaaacg ccccggaggg gcattcatcc
     6841 gcatcacgca aacatttcgc acagtgcaga catttcgcct ctctgcgtac agtctggatt
     6901 ttgccggaga tcgactccgg attagcacac cacgggcaaa gatgtggaca gcctttaaaa
     6961 aagaccaccg tacgaatgcc ctcgccgtca ttcagtgaat aacgctgaat gttgaaaatg
     7021 cgcgccacat cattgcggcg cgtttccaca acgttacagc tgatgcgctg tccggcggat
     7081 gatgtcatcc tggatctcct tcgacagttc gacaaagaag gcgctgtatc cggcaacgcg
     7141 caccaccagc ccggcataat cttgtgggcg ctgttgcgct tcccgcaacg tgtcggcgtt
     7201 caccacgtta aactgaatat gttgtaactt aagctgggta aacgcccgta agaagtcggc
     7261 cagtttgcgt aatcccgctt caccttccag ggtcgccgga gtgaatttca cgttcagcaa
     7321 cgtaccgtta gacagcagcg tattatcgag cttactgact gacttcagta ccgctgttgg
     7381 cccttgtgcg tcctggccca gcatgggtga caagccgccg tctgccagct gttctccggc
     7441 aaaacgaccg tctggcgtcg cgccaaccac cgatcccaac gggacgtgag cagaaacggt
     7501 atacgatccc ggcgtgaagt aaccgccgcg cgggttctgg tatttttcca cttctttgca
     7561 gtagtggcgc aacagctcgg cgctaatgtt atccacctcg tcgatatcgt taccgtattt
     7621 ctcaaagcgg ttaattaagc gagcgcggac tttttcgcct tctggcgttg caaagttggc
     7681 ttttaatacc gacagcaatt cgtcaaaact taaacgctgt tgatcaaaaa ccatcccttt
     7741 caacgcatgg agagaatcgc tcaggttggc gataccaatt ccctgtacgc cggagaagtt
     7801 ataacgcgcg ccgccatcgg taatatcgcg gcctttttcc agacaatcgc tgataaacga
     7861 tgagagcagc ggtacaggtg cccagtcgcg atggccgata tcacaaatat tgctgccctc
     7921 aaccatcagg gtgatgtagt ggctgatctt ggcgcggatc tgctccagta aaccttcata
     7981 agtcagcgca gcattgcctt cattttcatg caggcagatt tccatcactt tcagcagatt
     8041 aaacatcgcg atgtcatgca agccgtaggt tctgccggga atagataatt ccacacagcc
     8101 cactacggaa tagtcgcgcg catcttccag cgaaacgcca cggttgagga acgctggcac
     8161 caccacttca tcgttaaaga tttgcggaat accggtgccg aggcgaatgg tttctgcggt
     8221 tttcatcagg aacggcgtgt cgatcaaggc gttagtgcgc acccctaggt tcggttgcgg
     8281 taattgtacg ctttgatagg catcgaggca gaggaacgaa agcacgttca ccgcgctacg
     8341 tccgttctcg gttaacccgc cgagcagtgc ggtatagcca gtcgggaaac ctgcgaaata
     8401 acgcgcgctg ctggtggagc gcaacagcac aatgtcgttg catttcaccc ataaagattc
     8461 gagcagttct ttcaggaacg ccggatcttc gccctgggtt aaagatgcct gatagaacgg
     8521 caacatatac tggtcgaagc gccctaaaga cagcgaactg gcgttggatt cgtattgcag
     8581 aatgatgttc atgtaccaga ataactggca cgcctgccag aacgtctgcg gtttatgttc
     8641 ggcgttatgg cgggagatct ccgcaatagt cagcagttct tcgcgacgct gggcatcagt
     8701 gcagcctgcc gccatcgttt ccgccagttc ggcgtaacgc aaaatgtgtt tctgcgaggc
     8761 ttcgagcagt aacagcgctg cctgataaaa gtgattctcc ggctgttgct gacaatgttg
     8821 ctgcatctgc gccaccagct cccccagccc gtgattcagc aggcgcgggt aatcaataat
     8881 aatgtgcccc tggcctttat ccgtctggtt gatgctgaaa atctgcgtgc tggtcgcggc
     8941 ttttacttca tccgtcatct gcccgttgat gaaatctttc atcgaacgtt tttcccagta
     9001 cgggaacaac tcttcgcggt agatacgttt atcttcttcg ctgatagcaa agcggtcctg
     9061 cggacgcgtc gggaattgat ccagctcttt cagcagccag taagggtcca tttccggcga
     9121 cataatcccg gcgcgtggtt tcaccgtgcg gttaccggca atcagttctt catcacgaat
     9181 cgaaatttca acatgttcaa ggatatacgc tgttgctttc gcccggcgca atatcaccgg
     9241 ttcgccttcg gtttgccgat ggctggcggt ataaagcagc gcacgctcca gcgagatttc
     9301 acgggtattg gcaaacagtg cagttttgag gcgagagata cgattcgtca tgaggaactc
     9361 ctgtgggaaa gcgaacccgc ccttgcgagc gggagaagaa cttaagcggt ttgcgccagg
     9421 tgagcttcaa ttttgctcat aatggcgtca gcgcgtttta ccgcgtcgct gatgttgacg
     9481 cgcacaatgg tcttgcccgc aaaacgctct tcaaacttga tgccgatatc tttggtcaga
     9541 atcaccatat cggcgctcgc cacatcttcc gcagtcagtt cgttttccag accaatcgag
     9601 ccctgggttt ctactttcac ttcccagcct ttcgctttcg cggcactttc cagcgcctct
     9661 gccgccatat aggtatgggc aacaccagat ggacatgcgg ttactgcaat aatttttgtc
     9721 atggctcgtt cctcaattaa ttaatttcaa aatccagatc caggtcgtct tccttctcat
     9781 cagtcgaaga ccctttttta cgcgccagac ttttcagcac gttcacacaa atagccgtca
     9841 ccactgcccc gacagccact gctgcgatat aacccagctt gccttcaacc accggcagta
     9901 caatcagacc accccagcct gcatagcact gcgcaccgac cagcgccgca gttactgcac
     9961 cacaaactga accgaccatg atggaaggaa taacacgcag cggatcggca gcggcgaaag
    10021 gaatcgcccc ttcagtaacc ccaacgcacc ccatcaccag cgccgcttta ccggtttcgc
    10081 gctcttctgc ggagaaattt ttacgaccaa tcagcgtcgc cagccccatc cccagcggcg
    10141 ggatacaaat acccacggca gcaatggcca ccacggtata aacaccctga gcaacgcaaa
    10201 tcagcatgaa ggcataggcc actttgttaa ccggaccgcc catatcgaac gccagcatca
    10261 gacccatgat caccgccagc ataacaatgc tgccctgctg catcccctga agccactgag
    10321 tcaggctgtt ggtcaacgcc cctacaggct cgcccaagcc ccacatcatg atgcctgcgg
    10381 taatcagtgt gccgatgata ggaatgatga agataggcat caccgagcgc agaactttat
    10441 gcaccggaat tttcttcagg taatgcacca cgatgccgcc gataatcccg gcgatcagtg
    10501 caccaaagaa gcccgcacca aagctgttac caacccaggc accgatagcg cacggagcca
    10561 gcgcagaacg ctctgcaatg gagtaaccga tgtaagcggc gaggaaaggc accatcagcg
    10621 tcaaacctgc aacgccgata tcaaacagtt ttttcagatt cggatcggct acggcatccg
    10681 gcactgcgcc tttgccatac aacatgacgg aaaccgccag caaaataccg cccgatacca
    10741 cgaagggaat catgtgtgaa acgcccgtca ttaaatgctg acgggtattt ttcaggatct
    10801 gcaccaactc attcataagc tgctcccggg cttgcgaaag cccatgtcca tatttttgat
    10861 ggaggaaaca ataggcaatc gcaggcggag caaacagtgg ataaaaaggc catttactgg
    10921 acttttgtaa tatcagtaca aaaatgcgat ccgcctcata acttgcgata atccggaagc
    10981 atgccgcaaa acggcgtctt gtgagacgcg tcgcaaattg acggttacat tacatttgtc
    11041 cagcttttgg cagatttgtc acatggcagg ccgttgccgt gcgctcttat cctgtaaaca
    11101 gaaaaccgta ccgggagaga ggcaatggcc ctgattgtgg aatttatttg tgagctacct
    11161 aacggcgtac atgcacgtcc ggcaagccac gttgaaacgc tgtgtaatac tttttcatca
    11221 caaattgagt ggcataacct gcgcactgac cgcaagggca acgccaaaag cgcccttgcg
    11281 ctgattggca ccgacacgct ggcgggcgat aactgccagt tactgatttc cggggccgac
    11341 gaacaggaag cgcaccagcg tttaagccaa tggctgcgcg atgaattccc ccactgcgat
    11401 gcgccgctgg cggaagttaa atctgacgaa ctggaaccac tgccggtttc actgaccaat
    11461 ctgaatccgc aaattatccg cgcccgcacc gtgtgcagcg gtagcgcagg cggcattctg
    11521 acgccgatct cttctttaga tctcaatgcg ctgggtaatc ttcccgcagc caaagacgtt
    11581 gacgccgagc aatccgcact ggaaaacggc ctgacgctgg tactgaaaaa cattgagttt
    11641 cgtctgctgg atagcgacgg tgccaccagc gcgattctgg aagcgcaccg atccctggct
    11701 ggcgatactt ccctgcacga acatttactg gcaggcgtca gcgccggatt aagctgcgcc
    11761 gaagcaattg ttgccagcgc gaatcacttt tgcgaagagt tcgcccgttc cagcagcagc
    11821 tacctgcaag aacgtgccct ggacgtacgc gacgtctgct tccagttact ccagcaaatc
    11881 tacggtgagc aacgcttccc ggcaccgggc aaactgacgc agcccgccat ttgtatggct
    11941 gatgaactga cccccagcca gttcctcgaa ctggataaaa atcacctcaa aggattgtta
    12001 ctgaaaagcg gcggcaccac ctcacatacc gtaattcttg cccgttcgtt caacattccg
    12061 acactggttg gtgtagatat tgatgccctt actccgtggc agcatcaaac gatttatatc
    12121 gacggcaacg ccggggcgat tgtggttgag ccaggggaag ccgtagctcg ttattatcag
    12181 caagaagccc gcgtacagga cgcccttcgt gagcaacagc gtgtctggct gacccaacaa
    12241 gcccgtaccg ctgacggtat ccgcattgaa attgccgcta acatcgctca ctccgtggaa
    12301 gcgcaggccg cattcggcaa tggtgcggaa ggcgttggtt tgttccgcac tgaaatgctc
    12361 tatatggatc gcaccagtgc accgggcgaa agcgagctgt acaacatttt ttgtcaggcg
    12421 ctggagtccg ccaacggacg cagcattatt gtgcgcacta tggatattgg cggtgacaaa
    12481 cccgttgatt atctgaacat tcccgcagag gcaaacccgt tccttggtta tcgcgccgtg
    12541 cgtatttatg aagagtacgc gtcgttattt accacacaac tacggtcgat tctccgtgcc
    12601 tccgctcacg gcagcctgaa aatcatgatc ccgatgatct cttcaatgga agagatctta
    12661 tgggtgaaag aaaaactggc ggaagccaaa cagcaactgc gtaacgaaca cattccgttt
    12721 gatgagaaga tccagctcgg catcatgctg gaagtgccgt cggtgatgtt catcatcgat
    12781 caatgctgcg aagagattga tttctttagt attggtagta atgacctgac gcaatatctg
    12841 ctggcggtgg atcgcgataa cgctaaggtt actcgtcact acaacagcct gaatccggca
    12901 ttcttgcggg cgctcgatta cgccgtgcag gcggtgcatc gccagggcaa atggattggt
    12961 ctgtgcggtg aactgggagc gaaaggttcc gtgctgccgt tgctggtcgg tttagggctg
    13021 gatgaactca gcatgagcgc accgtcaatt ccggcggcga aagcgcgaat ggcgcaactt
    13081 gatagccgtg agtgccgcca gttgctcaac caggcaatgg cctgccgtac ttcgctggaa
    13141 gtggaacatc tgctggcgca attccgcatg acccaacagg acgcaccact ggtcaccgcc
    13201 gagtgcatca cgctggaaag cgactggcgc agcaaagaag aagtactcaa aggcatgacc
    13261 gataacctgc tgctggcggg ccgctgccgc tatccgcgta aactggaagc cgacttgtgg
    13321 gcgcgcgagg ccgttttctc taccggtctg ggctttagtt ttgccattcc ccacagcaaa
    13381 tcagaacaca ttgagcagtc caccatcagc gtagcgcgtc tgcaagcgcc ggtgcgctgg
    13441 ggcgatgatg aagcgcaatt catcatcatg ttaaccctga acaaacacgc tgcgggcgat
    13501 caacatatgc gcattttctc gcgcctcgct cgtcgcatca tgcacgaaga attccgtaac
    13561 gcgctggtta acgccgcctc tgccgacgct atcgccagcc tgctgcaaca tgaactggaa
    13621 ctgtaaaagg aaacatcatg gaactgtatc tggacaccgc taacgtcgct gaagtcgaac
    13681 gtctggcacg cattttcccg attgccgggg tgacaactaa cccgagcatt atcgctgcca
    13741 gcaaggagtc catctgggaa gtgctgccgc gtctgcaaaa agcgattggt gataagggca
    13801 ttctgtttgc tcagaccatg agccgcgacg cgcaggggat ggtggaagaa gcgaagcgtc
    13861 tgcgcgacgc cattccgggc attgtggtga aaatcccggt gacttccgaa ggtctggccg
    13921 ccattaagat gctgaaaaaa gagggtatta ctacgcttgg caccgcagtg tacagtgctg
    13981 cacaagggtt actggccgcg ctggcagggg caaaatacgt tgctccgtat gttaaccgcg
    14041 tagatgccca gggcggagac ggcattcgta cggttcagga gctgcaagcg ctgttagaaa
    14101 tgcacgcgcc agaaagcatg gtgctggcag ccagctttaa aacgccgcgt caggcgctgg
    14161 actgtttact ggcaggatgt gaatccatca ccctgccctt agatgtagcg caacaaatgc
    14221 tcaacacccc tgcggtagag tcagctatag agaagttcga acacgactgg aatgccgcat
    14281 ttggcactac tcatctctaa aggagcaatt atggaccgca ttattcaatc accgggtaaa
    14341 tacatccagg gcgctgatgt gattaatcgt ctgggcgaat acctgaagcc gctggcagaa
    14401 cgctggttag tggtaggtga caaatttgtt ttaggttttg ctcaatccac tgtcgagaaa
    14461 agctttaaag atgctggact ggtagtagaa attgcgccgt ttggcggtga atgttcgcaa
    14521 aatgagatcg accgtctgcg tggcatcgcg gagactgcgc agtgtggcgc aattctcggt
    14581 atcggtggcg gaaaaactct cgatactgcc aaagcactgg cacatttcat gggtgttccg
    14641 gtagcgatcg caccgactat cgcctctacc gacgcaccgt gcagcgcatt gtctgttatc
    14701 tacaccgatg agggtgagtt tgaccgctat ctgctgttgc caaataaccc gaatatggtc
    14761 attgtcgaca ccaaaatcgt cgctggcgca cctgcacgtc tgttagcggc gggtatcggc
    14821 gatgcgctgg caacctggtt tgaagcgcgt gcctgctctc gtagcggcgc gaccaccatg
    14881 gcgggcggca agtgcaccca ggctgcgctg gcactggctg aactgtgcta caacaccctg
    14941 ctggaagaag gcgaaaaagc gatgcttgct gccgaacagc atgtagtgac tccggcgctg
    15001 gagcgcgtga ttgaagcgaa cacctattta agcggtgtag gctttgaaag tggtggtctg
    15061 gctgcggcgc acgcagtgca taacggcctg accgctatcc cggacgcgca tcactattat
    15121 cacggtgaaa aagtggcatt tggtacgctg acgcagctgg ttctggaaaa tgcgccggtg
    15181 gaggaaatcg aaaccgtagc tgcccttagc catgcggtag gtttgccaat aactctcgct
    15241 caactggata ttaaagaaga tgtcccggcg aaaatgcgaa ttgtggcaga agcggcatgt
    15301 gcagaaggtg aaaccattca caacatgcct ggcggcgcga cgccagatca ggtttacgcc
    15361 gcactgctgg tagctgacca gtatggacaa cgtttcctgc aagagtggga ataacctact
    15421 ccaaactccc ggcttgtccg ggagtttgaa cgcaaaattg cctgatgcgc tacgcttatc
    15481 aggcctacgc aatctctgca atatattgaa tttgcgtgct ttttaggcca gataaggcgt
    15541 tcacgccgca tccggcactc tgtgccagta atctgaaact cctacgttgt tcaggagttt
    15601 tttatccgct tctggacatt ttctctacag aacaatcgtt atcccggaag gtcgaatctg
    15661 tgactcttat tttcacggac gaaacagatg aaagcttcac tggtgctggt cagcctgcta
    15721 acggcattta ccagccactc cttaaaatct cccgccgttc caccaaccgt ggtacaaatc
    15781 caggccaata ccaaccttgc catcgaagac ggtgccagac agcaaatcgg cagcacgcta
    15841 ttttacgatc cggcgtatgt gcagcttacc tatcctggcg gtgatgtccc gcaagaacgc
    15901 ggggtatgtt ccgatgtggt gatccgtgca ttgcgcagcc agaaggtcga tttgcagaaa
    15961 ctggtgcacg aagatatggc gaagaatttt gccgagtacc cgcaaaaatg gcagttaaag
    16021 cgcccggaca gcaacatcga tcaccgtcgg gtgcctaatc tggaaacctg gtttacccgc
    16081 cacgataaaa cacgccccat cagcaagaac cccagtgact atcaagcggg cgatattgtc
    16141 tcctggcgat tagataacgg gctggcacat attggggtgg tttcagacgg cttcgcccgc
    16201 gacggtacgc cgctggtgat acataacatt ggcgcaggtg cgcaggaaga agatgtgctg
    16261 tttagctggc gcatggtggg gcattatcgt tattttgtta aataagcgat cgccccacat
    16321 gaagaaaaaa ttcagatcct ttttacacta acggcttctt ttttcttacg gctcaccagc
    16381 gccagtgcca gcacaatcag cacaataccg ctaccttcaa cggcccccgg attctcaccg
    16441 agcagccacc aggaaaagag tacgccgcaa acgggaacgg ccagtgtgct taagctggca
    16501 atactggcag gcaagttttt cagcacaaac agccataagc tccacgccaa cgccgtcgcc
    16561 agaatcgcac tgtaggccag agcccagaac acagtgggct gccagtcaat ttcacgttgc
    16621 ggcaccagta aagcgaccac actcatcacc agcgccgcat agagcatctg ccaggatgtt
    16681 aacgacaata aatccacgcg cggatgacgg gcatacagac gtttagcaac aatcgcgctc
    16741 gccccccaac taacaccgga gaggattgcc agcatggcac ttttcatcga agagaaatcg
    16801 agttgccacg gctgcaacac caaaaataag ccgaaagcgg caatcagaat cgcgaaatat
    16861 tgtccacgtc gcaggcgttc accgagaaac aacgcggcga aaatcaccac ccagaacggc
    16921 atggtatagc tcaggatcgc cacctttccc gctccgccgc tgaccagcgc ccactgcgcc
    16981 aggccaacca tcccacaggt ttgtaacagc gcaatggcta aggtgtattt aaacggcgtc
    17041 gggcgcattc cgcgaccacg taataaaagg acgatgaata aaacgagagc gccgaaaatg
    17101 cagcgtaagg cggtaaagtc gaaggcaccg atgtagctgg tgacttgctt catgaaaatc
    17161 cagctataac tccagataag tgtgagcaca accaggccac tgattgccag tgggttgctc
    17221 tttcctgcgg cagacatgag agatccagtg tgtagttttc ctgtggacac tatacgccgg
    17281 taaagaaact tcagcgactg cctgaaaggc agtcgctgaa gaaggtcaga ttacagcagg
    17341 tcgaaacggt cgaggttcat cactttcacc catgccgcca cgaagtcttt aacaaacttc
    17401 tcgtgggcat cgctgctggc gtaaacttca gccaccgcac gcaggacaga gttagaacca
    17461 aataccagat ccgcacggct ggcagtgtat ttcacttcgc cggtttcgcg gtcacggcct
    17521 tcgaacagct ctttcgattc gtcggtcgct ttccactcgt aacgcatatc cagcaagttc
    17581 acgaagaagt cattgctcaa tacgccaacg cggtcagtga agacgccgtt tttgctgcca
    17641 tcgaagttgg cacccagtac acgcatcccg cccaccagcg cggtcatttc cggcgcggtc
    17701 agggtcagtt gctgggcttt atcaatcagc agtgactcgg tggtggaaac atccagacga
    17761 gcgcgatagt tacggaaacc gtcagcaatt ggctccagca gttcgaacat ctcaatgtca
    17821 gtctgatcct gacgcgcatc aacgcgaccc ggcgcaaacg gtacatgaat gctcaaacca
    17881 gcggcgcttg cggctttctc aacgccaacc acacctgcca gcacgatgat atccgccagc
    17941 gaggctttac cagactcttt ctgaattttc tccagaactg gcagcgcacg aacggctgct
    18001 gcgttcacat cccagtcgcg ctgcggcatt aatgccagac gcgcaccgtt ggcaccaccg
    18061 cgtttgtcgc caccacggaa ggtagaagca gaagcccagg ccaccgatac cagctcacta
    18121 acagacagac cagaatccgc aatcgcgaat ttcagatcga taatgtcctg ctcggtcggg
    18181 ttgtagatcg gctgcggcag cggatcttgc cagatcagat cttctttcgg cacttccggc
    18241 ccgatgtagc gagatttcgg ccccatatcc ctgtgcgtca gtttgaacca ggcacgggca
    18301 aaggcttcgt tgaacgcctg cggatcgttg aggaaacgac gagagatctt ctcgaactca
    18361 ggatcaaagc gcagcgtcag gtcggtcacc agcatcgtcg gtttacgttt cttcgacgga
    18421 tcaaacggat ccggaataat ttccggtgcg tctaccgctt cgaactggat tgcgccagcc
    18481 gggctgcggg tctgtaccca ctcatacttg aacaggttct cgaagaaata gttgctccac
    18541 tgggtcggcg tctgggtcca gactacttcc agaccagagg taatggcatc tgcgccaacg
    18601 ccgctgccgt aagtgctcgc ccaacctaaa ccttgttctt caatcggtgc agcttctgga
    18661 tcaggaccta catttgatgt cggaccagca ccgtgggttt tacccagcgt atgaccaccc
    18721 gcaatcagcg ccacggtttc ttcgtcgttc atacccatgt tgccgaaggt cgcgcggata
    18781 gctgctgccg cagaaagcgg ttcgccgctg tgatccgggc cttccgggtt aacgtaaatc
    18841 aggcccatct cggttgcacc cagcggtgct ttcgccagcg cttccggatg acggtgagtc
    18901 agccaggctt tttcatcacc ccagttaaca tccagatccg gttcccagac gtcttcacga
    18961 ccggcaccaa aaccgaaggt acggaagccg gagttttcca gcgccacgtt acccgcgagg
    19021 ataaacaggt cggcccagga gattttctga ccatatttct gtttgattgg ccacaacagg
    19081 cgacgtgctt tatcgaggct tacgttatcc ggccaggagt tcagcggtgc aaaacgttgc
    19141 tgaccacgac ccgcgccgcc gcgcccatcg attgaacggt aagtacccgc accgtgccag
    19201 gccatacgaa taaacaaacc ggcgtagctg ccccagtcgg ctggccacca cggttgagat
    19261 tctgtcagca gggctttcag atcttttttc aggccgtagt aatctaattt gctgaattct
    19321 ttgcggtagt caaagtcctc acccagcggg ttagaacgat tagaatgttg gtttaacagg
    19381 tcaacacgga gttgatttgg ccaccagtcg cgagtggttg tgcccgcccc cgcgctctgg
    19441 tcgtgaccgc cctgatggaa cgggcatttg ccagtggctg tcgtgttatg gatatcgtct
    19501 gacgtgctca tcaatgtgct cccctctaca gtgttaccgt tacgatacac agcgttagag
    19561 agaagttata attgaattaa tccacggatg cgatagttga gatcttacat attgttggtt
    19621 aaagagatgt agatcaaatt gatcgtaatt aagctttttt gattatgtcc gaattcggac
    19681 aaatgcttta taaaaaaggg ttttagcttc atatccttca agtaatgagg gagaaaacaa
    19741 ggctgataac cttattttcc cgccctcatt tcgaggcagc attttgtgct ctgtttaaaa
    19801 tttgtgatca ctgtgtaatt ttcgcaaaag ccacactatt tataaaccag gtcgaacacc
    19861 cagcgtatgg caaatcgcgt aactcatttc agcgcggtta agcgtataga agtggaaatc
    19921 tttcactcct tcacggctta aaatcttcac catatccatg gcgatattcg cgcctaccag
    19981 cttgcgggtt tcggcgtcat catccaggcc gtcgaacatt tgcgccatcc acgccggaat
    20041 acgcacgttg gtcatatcgg caaatttctt cgcctgttta aagttagata ccggcaaaat
    20101 tcccggaata atttccacat caatgcccgc cgatacacag cggtcacgaa aacgcaggta
    20161 gctttcgaca tcgaagaaga actgagtaat cgcgcggttg gctccggcat cgactttgcg
    20221 tttcagatta agcaaatccg cctgagcgct ttttgcttcc gggtgaactt ccggatacgc
    20281 cgccacggag atatcgaaat ctgccacttc ttttaatagc gtcaccaggt cagaagcata
    20341 catttctggc ttaccacttc ccggcggcag atcgccacgc agcgccacga tatgacgaat
    20401 accgttattc cagtagtcgc gtgcaatggt gcgcaactcg tcgggcgtcg catcaatgca
    20461 agtaagatgc ggtgccgctt ccagaccagt gcgatcttta atgcctttaa taatgctgtg
    20521 cgtacggtcg cgctcgccgg agttcgcgcc ataggtcacc gatacaaact tcggtttcag
    20581 gctgctaagg cgatcgatgg agttccacag ggtctgctcc atttcactgg tacgcggcgg
    20641 gaaaaactcg aacgaaacgt taatctgccc ctggacttct gccaggctct gattcagggc
    20701 atcccgctgg ctggcgtgaa aaaagctcat accttacctc atcaatcgct tgtcgttata
    20761 tgttgtgttg tgcacatcta tccgtttaga cgtccagatg taaagatgaa ggaaaagccg
    20821 aagggcgtca accgaaaaat gaccaatgat ggtgaggaaa aatcagggaa gatgaaaaaa
    20881 cttcaggatg gtaagaaaaa gaaaatccgg tatgcaaagc aaaccggact gcaaatatta
    20941 actaaccatg tacttactgc ttcttgccgg attcgtcgta aacgcttcat ccggcctaat
    21001 cctatgataa ttcaatatat ttcattagaa accatagacc cgaaaacatg agtaccgggc
    21061 attattaaat ttctgaaatt acaacaactg tgccagccgg ttgatatccg actgaatcgc
    21121 cccggcggtg acgtcgcgcc ccgcgccagg cccacggatc accagcgggt tgtcgcgata
    21181 ccagcggctt tcgatggcaa agacgttatc gcacggcagc agtgatgcca gcggatggtc
    21241 ttcacgcacc gcctccacgc ccacacgcgc tttgccgttg gcatcgaaac gcgccacgta
    21301 gcgcagcacc agccccattt cgcgggccgc ttccagccgt tgcaccatct gctcgttaag
    21361 ttcatcgccg ttttcaaaga agtggtcgat gctgccgcct tcgcaatggg caggcaccag
    21421 cgattccacg cgcacctgat ccggttcgat gtcgtaaccc gcttcacgcg ccagaatcac
    21481 cagcttgcgc atcacgtctt tgccggagag gtcgtcacgc ggatcaggct cggttaagcc
    21541 ctgctgccac gcctgatcca ccagctcggt aaacggcacg ctaccgtcga attgcaggaa
    21601 cagccaggag agcgtgccgg agaagatccc gctgatcgac aaaatagtat cgccgctgtc
    21661 gatcagatcg cgcacggtgt ggttgatcgg caagcccgca ccgacggtgg cgttgtacag
    21721 ccagtgacgc ccggtttttt cgaaggcgtc gtggatctgg cgatatttat tgctgtcgct
    21781 ggctcctgcc agtttgttgg cgctaatgac gtggaaaccg tggctggcga aatcgagata
    21841 ctgatcagca agctgctggc tggcggtaac gtccagcacc actaaatcat catacggatg
    21901 ggcgcgcatc cacaggaaaa gcgactcttc atcctgctcg accgcttcat cgttgaagaa
    21961 ggctaacgcg cggctggcgt ccagcccgtc ataactcaac agactgcggc ggctgtccac
    22021 cacgcctgcc agcacaaatt caaagccggt acgcgccgaa agcgtgctct gctcgcgggc
    22081 gaacagttcc agccagcggg aaccgatatt gcctttgccg aacaatacca ggccgatgcg
    22141 tttttctgcg cggaagacgg actgatgcag gccctgaatc aggctttcgg tcgggccggt
    22201 gcgcagtact gccaccaggc tgatgccgtc atcggactgc caggtaaact cgaccggctg
    22261 gcctttcagt tgctgccaga agcggtggca atgcagcggg ttacgggtga cacccgcccc
    22321 gaccatcgcc accagcgcca gcccctgacg caggcgcagt tcgccaggta atcccgcttc
    22381 gtcgaggatt ttcagcgcac tgtcggccac ttctgaggtg tagcaaaatt gcagcaactg
    22441 gcgatcgtta tgcacgccaa ccgccagtgg acgcacctgg gcacgtttca ggatctggtc
    22501 gatctcttta tgcgccagtt tgaaatcctg gctggtgggc acctgaaact caatcaagca
    22561 gacatcatcg tggctggtga caatacgcgc accagtaccg gaggccagca cgcgttcaat
    22621 gcgcgtggag ccttgatccg gcgtgtagct acagcgcagc tgcaggtcga tttcgctacc
    22681 ggatactggc tgtaaagtac gggcgtgaag aacgggagcc gccagacgcg ccagttcgct
    22741 ggcctcatcc agacgcagca acggcagcag acaggcatct ttcactttac gcggatcggc
    22801 actgtatacc ccggcgacgt cgctccagat ggttacacga gaaacacccg ccagcgcacc
    22861 gatttgtgtc gcggaatagt cggaaccgtt acgccccagc agcaccgttt caccggcgtt
    22921 gttacggctg ataaacccgg tcaccaccag acgtttaccc ggatgttgta ccagtagctg
    22981 ttgcagcaac gggtaagaaa gcccttcatc aacctgcggt tgcgcagcgc gttcagcgcg
    23041 taaaaactca cgggcatcaa gccaggccgc tggcagccct tgttgattaa gtaccgcaga
    23101 catcaggcgc gccgaccaca cttcaccgtg gcccaccact tccgcataca ctgcgtcgtt
    23161 aataccgctg tcgagcagcg ccgccaggcg ctcaagatcg ctgacaaaag cgctaatgag
    23221 gctatcggct tcttcggcgg gtagcaggcc gctaatcaga tcgcactgat agcgacgcag
    23281 cgtttgttga acctgatgcg cagagagacg atcggtctgg cttagtttca accagttaat
    23341 caactggtta gtggtgctac cggcggcgga aaccaccatc atatcgtcag gctgagagta
    23401 ctccgccata atgcccgcga cacgcaaata acacttcaca tcagccagac tactgccacc
    23461 aaatttatgc agctgacgac ctttcgcccc tgcctgcgca atcacactca tttttacccc
    23521 ttgtttgcag cccggaagcc attttccagg tcggcaatta aatcttcgcc atcttcaata
    23581 ccggtggaga tacgcagcag cgtctcggag atcccggcgg cagcacgcgc ttctggtgcc
    23641 atgcctgcat gtgtcatggt tgcggcgtga gagattaaac tttccactcc ccctaatgat
    23701 tccgccagcg taaacaacga cagcccgccc aggaaacgac gcagcgtctg ctcatcgcca
    23761 tccagttcaa aactcaacat tgcgccaaag cctttttgct gacgcgcggc aatttcatgc
    23821 ccctgatttt ccggcagcga cgggtgatac agttttttca ccaacggctg ggtttgcaga
    23881 tatttcacaa tcgcctgcgc gttgcgctgc gccagctcca tacgcggcac cagcgttcgc
    23941 aacccgcgta gcagcagata gctgtcaaac gcgccgcccg tcacgccaat attgtttgcc
    24001 caccaggcaa gttcagtgac aacgtccggg tctttagcaa tcaccacgcc ggccaccacg
    24061 tctgagtgac cgttcagata tttcgtacat gaatgcaaca ccagatcggc acccaatgcc
    24121 agcggatttt gtaatgccgg gcttaagaag gtgttatcca ccacgctcac cgccccgact
    24181 tcccttgcca gatggcagat tttcgcaata tccacgacgc gtaacaatgg attacttggg
    24241 ctttctacca gtaccagttt gggtttttct gccagcgctg cccgtaatgc ctgttcatcg
    24301 ccttgatcaa caaacaacac gcgatagcaa ccgcgtttcg ccaggctgtc gaacaggcga
    24361 tagctaccgc cgtagcagtc gtgcggcgca accagcagat cgccaggttt caaaaagacg
    24421 gtcgttacca ggtgaatcgc ggacatgccg gtattagtaa gtactgcacc agcaccgcct
    24481 tccagttctg ccagcgcacg ctgaaccaca tcgcgcgttg ggttgccgcg acgcgagtaa
    24541 tcatgcgcgc gcggttcatt aaatccggta aagttatagg tgctggaaag atggatggga
    24601 gggacaacgc aaccatactg ttcgtcgtca tttaacccgc tacgcactgc gatggtggcc
    24661 tgtttacgcg tcatgtgatg aagttccctg ggcttggtcg gtgaaatgtc aggcaccaga
    24721 gtaaacattg tgttaatgga cgtcaataca tctggacatc taaacttctt tgcgtataga
    24781 ttgagcaaat cccaaatagc cgttaaaatt atatgcatta tcacgccgac aggtgcatta
    24841 cacgatgtca cggtaacgcc tgtacggtaa actatgcggg tttacggtca gtacccacat
    24901 aaattgtgtg gtctggtctc aatttattga cgaagaggat taagtatctc atggctgaat
    24961 ggagcggcga atatatcagc ccatacgctg agcacggcaa gaagagtgaa caagtcaaaa
    25021 agattacggt ttccattcct cttaaggtgt taaaaatcct caccgatgaa cgcacgcgtc
    25081 gtcaggtgaa caacctgcgt cacgctacca acagcgagct gctgtgcgaa gcgtttctgc
    25141 atgcctttac cgggcaacct ttgccggatg atgccgatct gcgtaaagag cgcagcgacg
    25201 aaatcccgga agcggcaaaa gagatcatgc gtgagatggg gattaacccg gagacgtggg
    25261 aatactaaag cggaaaagag cggcgcggag ttaaccagcc gcttttttca tacaaggacg
    25321 tttaacgatg aaaatcaggc tactgatcct cagcctgctg gtttctgtgc ctgcctttgc
    25381 ctggcagccg caaactggcg acatcatctt tcaaatctct cgctcatcgc aaagtaaagc
    25441 gatccaactg gcgacccatt ccgattacag ccacaccggt atgctggtga tgcgtaacaa
    25501 aaagccctac gtttttgaag cggtcggccc ggtgaaatac accccgctca agcagtggat
    25561 tgcccatggt gaaaagggca aatacgttgt tcgccgcgtt gaaggcggac tcagtgttga
    25621 acaacagcaa aaactggcgc aaacggcgaa acgttatctc ggcaaaccgt atgacttcag
    25681 cttctcatgg agcgacgatc gccagtactg ttcggaagtg gtgtggaagg tttatcagaa
    25741 tgcgctggga atgcgcgtgg gcgaacagca gaagttgaaa gagttcgacc tgagcagccc
    25801 acaagttcag gcaaagctca aagaacgtta cggcaagaat attccgcttg aggaaacggt
    25861 ggtctcgcca caagccgttt tcgatgcgcc acaactcacc acggtcgcca aagaatggcc
    25921 gctgttttcg tggtaatgga tgataaaaaa aataattccc gccgtagtct gtttgttgct
    25981 ttgtagttgc acgacctttc cgcaagctgt cgctccggta aacggtgatg cctccaatta
    26041 gttgaattaa ggatataata ctcgctttta aggttgtttc atggtcacag ttaaccttca
    26101 ttgtccccgt tgccagtccg ttcaggttta ccgtcacggg cagaacccca aaagacatga
    26161 tcgattccgc ggccgtgact gtcaccgggt atttcagctc agttactgtt acgagccccg
    26221 taaaccggga gttaaagacc aaatcgctga aatggctttc aacggtgccg gggttcgcta
    26281 tactgcccgt acgctgaaaa tcggcattaa tacagtcatc cgaactttaa aaaactctcg
    26341 ccacgaagaa taacgtcatc cccggtcgct catgctgatg tggcactcat ctgcgaactt
    26401 agtcagcaat ggagctttgt cggcagtaaa gcgcgacagc actggctctg gtacgtgtac
    26461 aacacgaaaa ctggtggtgt tctggcctac acttttggcc cccggactga tgaaacctgt
    26521 cgtgaattgc tggcgctgct taccctttta ccatcggcat gttgaccact gacgaatggg
    26581 gtagttacgc tagagagtta ccaaaggaga agcatctgac tggtaagatt tttactcagc
    26641 acattgagcg taacaatctg accctgagaa cccgaatcaa acgtctggcc cgcagaacca
    26701 tctgtttctc gcgttctgta gaacttcatg aaaaagtcat cggtgctttt atcgaaaata
    26761 tatgttctac taattggagt catcaccctt ctgatgagct ttccttggat gattaccaga
    26821 ctgaatacac agccaaagat ttttacctca ttctgctctt ttttctcaaa tgacatatat
    26881 tatattttac atcatattca taggcatatg acattccccc gccccccatt atgtcattgt
    26941 gggttgatgt tgggctttca taaaaaaata cat
//
LOCUS       DAZFKF010000066        26876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFKF010000066 DAZFKF010000000
VERSION     DAZFKF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.066"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(346..2919)
                     /gene="clpB"
                     /locus_tag="ACHKA1_003247"
     CDS             complement(346..2919)
                     /gene="clpB"
                     /locus_tag="ACHKA1_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO5680857.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(3049..3780)
                     /gene="yfiH"
                     /locus_tag="ACHKA1_003248"
     CDS             complement(3049..3780)
                     /gene="yfiH"
                     /locus_tag="ACHKA1_003248"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO5680858.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAADAKASTAFIQHGDKYLADIYLLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3777..4757)
                     /gene="rluD"
                     /locus_tag="ACHKA1_003249"
     CDS             complement(3777..4757)
                     /gene="rluD"
                     /locus_tag="ACHKA1_003249"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO5680859.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEAMRADFEEHKDEVDWL"
     gene            4892..5629
                     /gene="bamD"
                     /locus_tag="ACHKA1_003250"
     CDS             4892..5629
                     /gene="bamD"
                     /locus_tag="ACHKA1_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO5680860.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5900..6241
                     /gene="raiA"
                     /locus_tag="ACHKA1_003251"
     CDS             5900..6241
                     /gene="raiA"
                     /locus_tag="ACHKA1_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO5680861.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6344..6469
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6345..6392
                     /gene="pheL"
                     /locus_tag="ACHKA1_003252"
     CDS             6345..6392
                     /gene="pheL"
                     /locus_tag="ACHKA1_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO5680862.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6491..7651
                     /gene="pheA"
                     /locus_tag="ACHKA1_003253"
     CDS             6491..7651
                     /gene="pheA"
                     /locus_tag="ACHKA1_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO5680863.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7694..8815)
                     /gene="tyrA"
                     /locus_tag="ACHKA1_003254"
     CDS             complement(7694..8815)
                     /gene="tyrA"
                     /locus_tag="ACHKA1_003254"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO5680864.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPALRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8826..9896)
                     /gene="aroF"
                     /locus_tag="ACHKA1_003255"
     CDS             complement(8826..9896)
                     /gene="aroF"
                     /locus_tag="ACHKA1_003255"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO5680865.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10106..10471
                     /gene="yfiL"
                     /locus_tag="ACHKA1_003256"
     CDS             10106..10471
                     /gene="yfiL"
                     /locus_tag="ACHKA1_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO5680866.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASAIRLN"
     gene            10621..11139
                     /gene="yfiR"
                     /locus_tag="ACHKA1_003257"
     CDS             10621..11139
                     /gene="yfiR"
                     /locus_tag="ACHKA1_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO5680867.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11129..12355
                     /gene="dgcN"
                     /locus_tag="ACHKA1_003258"
     CDS             11129..12355
                     /gene="dgcN"
                     /locus_tag="ACHKA1_003258"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO5680868.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12371..12853
                     /gene="yfiB"
                     /locus_tag="ACHKA1_003259"
     CDS             12371..12853
                     /gene="yfiB"
                     /locus_tag="ACHKA1_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO5680869.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12930..13277)
                     /gene="rplS"
                     /locus_tag="ACHKA1_003260"
     CDS             complement(12930..13277)
                     /gene="rplS"
                     /locus_tag="ACHKA1_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO5680870.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13319..14086)
                     /gene="trmD"
                     /locus_tag="ACHKA1_003261"
     CDS             complement(13319..14086)
                     /gene="trmD"
                     /locus_tag="ACHKA1_003261"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO5680871.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(14117..14665)
                     /gene="rimM"
                     /locus_tag="ACHKA1_003262"
     CDS             complement(14117..14665)
                     /gene="rimM"
                     /locus_tag="ACHKA1_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO5680872.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14684..14932)
                     /gene="rpsP"
                     /locus_tag="ACHKA1_003263"
     CDS             complement(14684..14932)
                     /gene="rpsP"
                     /locus_tag="ACHKA1_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO5680873.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15069..16430)
                     /gene="ffh"
                     /locus_tag="ACHKA1_003264"
     CDS             complement(15069..16430)
                     /gene="ffh"
                     /locus_tag="ACHKA1_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO5680874.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16597..17388
                     /gene="ypjD"
                     /locus_tag="ACHKA1_003265"
     CDS             16597..17388
                     /gene="ypjD"
                     /locus_tag="ACHKA1_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO5680875.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17409..18695
                     /gene="yfjD"
                     /locus_tag="ACHKA1_003266"
     CDS             17409..18695
                     /gene="yfjD"
                     /locus_tag="ACHKA1_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO5680876.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSILLAPLQILMMPLVWLLNAITRMLMRIMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18750..19343)
                     /gene="grpE"
                     /locus_tag="ACHKA1_003267"
     CDS             complement(18750..19343)
                     /gene="grpE"
                     /locus_tag="ACHKA1_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO5680877.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19466..20344
                     /gene="nadK"
                     /locus_tag="ACHKA1_003268"
     CDS             19466..20344
                     /gene="nadK"
                     /locus_tag="ACHKA1_003268"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO5680878.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNILSTKLGWSKKLF"
     gene            20430..22091
                     /gene="recN"
                     /locus_tag="ACHKA1_003269"
     CDS             20430..22091
                     /gene="recN"
                     /locus_tag="ACHKA1_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO5680879.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22240..22581
                     /gene="bamE"
                     /locus_tag="ACHKA1_003270"
     CDS             22240..22581
                     /gene="bamE"
                     /locus_tag="ACHKA1_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO5680880.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22643..22933)
                     /gene="yfjF"
                     /locus_tag="ACHKA1_003271"
     CDS             complement(22643..22933)
                     /gene="yfjF"
                     /locus_tag="ACHKA1_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO5680881.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22923..23360)
                     /gene="ratA"
                     /locus_tag="ACHKA1_003272"
     CDS             complement(22923..23360)
                     /gene="ratA"
                     /locus_tag="ACHKA1_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO5680882.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23531..24013
                     /gene="smpB"
                     /locus_tag="ACHKA1_003273"
     CDS             23531..24013
                     /gene="smpB"
                     /locus_tag="ACHKA1_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO5680883.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24228..24590
                     /gene="ssrA"
                     /locus_tag="ACHKA1_003274"
     tmRNA           24228..24590
                     /gene="ssrA"
                     /locus_tag="ACHKA1_003274"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24774..25967
                     /locus_tag="ACHKA1_003275"
                     /pseudo
     CDS             24774..25967
                     /locus_tag="ACHKA1_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101258.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
     gene            complement(25970..26815)
                     /locus_tag="ACHKA1_003276"
     CDS             complement(25970..26815)
                     /locus_tag="ACHKA1_003276"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO5680884.1"
                     /translation="MWTLPLPDDADRMSELLTALTYKNGNPKYALTQDEKDKIETIYT
                     LYHDRKATPCAEFLAEEISDETQNAIKTAYSEVQINGRLSTLRSRILLSSKKCPLCGI
                     ESSTDLDHYLPESIYKCLAVYSRNLIPTCHKCNNKKRAGVAENGVGFIHVYYFDEPDG
                     VFFVANTEMIDGALVIDFNINMIEGMTREVYDSLCFQNRVVNLNERLKMEANDFLGGL
                     YTSLEETYNSAGQEGLRLFLKRTRDDYTRRYGLNHWRRALIDSLSDNVEFLDAGFITA
                     LGTPE"
BASE COUNT         6897 a         7019 c         6248 g         6712 t
ORIGIN      
        1 atcttcgagt gcccacacag attgtctgat aaattgttaa agagcagtgc cgcttcgctt
       61 tttctcagcg gtacggggtg tgcataatac gctttcccgc tacagagtca agcatttatt
      121 tttgctttct ctgccggaat tctcaggaga accccgctga cccggcggcg tgtttgccgt
      181 tgttccgtgt cagtggtggc gcattatagg gagttattcc ggcctgacaa gcgaaaaata
      241 taaaaacttt atcgttcgct cacttttcag gcaaaacatc ttaaatatag tcttttccgt
      301 ctaacttata gacaaaaacg agccccgaag ggctcgtttt atcatttact ggacggcgac
      361 aatccggtct tcattaactt ccaggcgaat cactttaccc ggaaccaatt caccagacag
      421 tatttgctgt gccagcgggt tttcgatctg ctgctgaatt gcacgtttca gaggacgtgc
      481 accatagacc ggatcgtaac cgttctcgct cagcagtttc agcgcctcgt cagaaatgtg
      541 gatttcataa ccacgttctt ccagacgttt atacagacgt ttcaactgaa tctgcgcaat
      601 cgacgcaatg tgctgttcac ccagcggatg gaagaccacc acttcatcga tacggttaat
      661 gaattccgga cggaagttat ggctaaccac accgagcacc agctctttca tgtgcgcata
      721 atccagttca ccgaagcgtt cctgaatcaa atcggaaccg aggttagagg tcataatgac
      781 aaccgtatta cggaagtcga ccgttctccc ctgcccgtca gtcagacgcc catcatccag
      841 tacctgcaac agaatgttga agacatccgg atgcgctttt tccacttcat ccagcaggat
      901 aacggaatac ggacgacgac gcacagcttc ggtcaggtag ccaccttctt cataaccgac
      961 atatcccgga ggcgcaccaa ccagacgaga caccgagtgt ttctccataa actcggacat
     1021 atcgatacgg accatcgcct cgtcgctatc aaacataaag ttcgccagcg ccttacaaag
     1081 ctctgttttc cccacaccag ttgggccgag gaacaggaat gaaccaatcg ggcgatttgg
     1141 atccgccagc cccgcacggc tacgacgaat agcgttagat accgcatcaa ccgcttcgtt
     1201 ctgaccaatt acgcgatggt gcagttcttg ctccatacgc agcagttttt cgcgctcgct
     1261 ttccatcatg cgagaaaccg gaatccccgt ccaacgcgcc agcacttcag caatttcggc
     1321 gtcggtcact ttattacgca atagacgcat agttttgcct tcgagctgcg ttgcggcttc
     1381 cagttgcttt tccagttccg ggattttgcc gtattgcagt tcagacatcc gcgccaggtc
     1441 ccccacacgg cgagcctgtt caatagcgat tttcgcctgt tccagttccg ctttaatggt
     1501 ctgcgtacca gaaagcgatg ccttctctgc tttccactct tcttctaact cggagtactg
     1561 acgttctttg tcgctcagtt cttcgttgag catatccagg cgttttttac tggcttcatc
     1621 agactctttc attaacgcct gttgttccag tttgagctgg atgatacgac gatcgagtcg
     1681 gtcgagttct tctggttttg agtcaatttg catacgaatg ctggatgctg cttcatcgat
     1741 caggtcgata gctttatccg gcagctgacg gtcagcaatg tagcgatgag acaacgtcgc
     1801 cgctgcaaca attgccgggt cagtaatttg cacatggtgg tgcaattcgt aacgttcttt
     1861 caggccacgc agaatcgcaa tggtatcttc aacagaaggc tcggcaacaa acactttctg
     1921 gaaacgacgt tccagcgcag catctttttc gatgtactgg cgatattcgt caagcgtagt
     1981 ggcacctacg cagtgcaatt caccacgcgc cagcgccggt ttcagcatgt ttccggcgtc
     2041 cattgcgcca tcggctttac ccgcgccgac catggtatgt aattcgtcga taaacaggat
     2101 gacgttgcct tcctgtttgg caagatcgtt aagcacgcct tttaaacgtt cttcaaactc
     2161 accgcgatat ttcgccccag ccaccagcgc gcccatatcc agcgccagta cccggcggcc
     2221 tttcaaccct tccggcactt cgccgttgat aatacgctgc gccagacctt caacgatggc
     2281 agttttaccg acgccgggtt caccaatcag taccgggtta tttttagtac gacgttgcag
     2341 cacctgaatg gtacggcgaa tttcttcatc acgaccaatc accggatcga gtttgccctg
     2401 ttcggctcgt tcggtaaggt cgatggtata ttttttcaaa gcctgacgtt ggtcttcagc
     2461 accttgatcg ttcacgcttt cacctccacg catttgttca atcgcttgag taatgttggc
     2521 ggtggtcgcc cctgctgctt tcaggatgtc ggccagcgtg ccgcgagact caagtgccgc
     2581 cagaacgaac agttctgacg agataaagtt atcaccacgt ttttgcgcca gcttgtcgca
     2641 aagattaaga acgcgcacca gatcctgtga tggctggaca tcaccaccag taccttcaac
     2701 ctgcggtaaa cgatttaatg cctgattgat atctgtgcgc aactggccag catttatgcc
     2761 agctgatgtt aacaaaggac taaccgaccc cccttcctga ttcagcaggg cgctcattaa
     2821 atgaagtggt tcgatgaatt ggttgtcgtg cccgagtgca agtgattggg catcggcaag
     2881 agcaagctgg aatttattag taagacgatc cagacgcata actcctccca taacggatca
     2941 aagttgctac tggagattaa atgaggtcat ccctcaatta ttcaaggtta ttgaccagat
     3001 taatgtgaaa agaaaatcac gcgtaccgga tcgtcttgat tctttaggtt atatcagcca
     3061 aatgaaactt gccatacgcc cggtggtctt gtcgcgacga taagagaaga aagtctcatt
     3121 ttccgtatat gtacaacggt cgccgccgaa aatttgttca acaccaacgc tcgccagacg
     3181 ctgccgggca agcagataaa tatccgccag atacttatca ccatgctgaa tgaaagctgt
     3241 actcgctttg gcgtctgctg ccataaacgc ctcgcgaacc tccgccccca cttcgaacgc
     3301 gcgtggacca attgccggcc ctaaccaggc gagaatattt tccggattat cagcaaaaca
     3361 ggaaactgtc tcttccagca cgcctgcgca cagtccacgc cagccagcat gagcggcggc
     3421 gacttccgtt cccgctcgat tgcaaaacag cacagggagg cagtcggcag tcatcactgc
     3481 gcaaaccgtg ccaggcgtat tgctataaga ggcatctgct cgttttgagg cataaggttc
     3541 gccagtaagc ttaagcacat ctttgccgtg tacctgctca agccagaccg gtttagaagg
     3601 caaattgccc gcagcaaaaa gtcgcttgcg attctcctca acatgatccg ggttatcgcc
     3661 acaatgggca ccgaggttga gcgagtcata cgggggcaag ctcacgccgc cgatacgagt
     3721 agagctacaa gccgcaacac cttttggcag cggccactgc gggacaatca gcttactcat
     3781 aaccagtcca cttcatcctt atgttcttcg aaatcggcac gcatcgcctc aatcagctcc
     3841 accatatctt gtggaatagg cgcatgccat tccatttcga tgccggagat cggatgataa
     3901 agacgcagca tggttgcatg aagcgcctgg cggtcaaact tacgcagcgt ggagataaac
     3961 gcttccgaag caccttttgg cggacgcgga cggccaccat aaaccggatc gcccaccagc
     4021 ggatgagtga tatgggccat atgcacgcgg atctggtgcg tacgtccagt ttccagacgc
     4081 aaccgcagac gcgtgtgcac acggaagtgt tccatgatgc gatagtgagt caccgctggt
     4141 ttgcccatcg gatgcaccgc catgtgagta cgtttggtcg ggtggcgact gattggctca
     4201 tccaccgtac cgccagcggt catatgacca atcgccaccg cttcgtactc acgagtaatt
     4261 tcacgccttt gcaaagattc gactaaacgc gtctgagccg gaacggtttt tgccacaacc
     4321 atcaggccag tggtgtcttt atccagacga tggacgatgc ccgcacgcgg tacatcggca
     4381 ataggcggat aataatgaag caacgcattc agtaccgtgc catccgggtt acccgcgcca
     4441 ggatgaacca ccaggtcgcg cggtttatta atgacgatga tgtcttcatc ttcatagacg
     4501 atatccagcg ggatatcctg cggttcaaaa cgcgcttctt cttcaatctc tgcgttgatg
     4561 gcaacctgct cgccacccaa tactttttct ttcggcttat cacaaagttt gccgttaacc
     4621 agcacgcgct ggtcgaggat ccattctttt attcgcgaac gtgaataatc cgggaacatt
     4681 tcggccaaag cctgatctaa gcgttgaccg agttggtttt cggacaccgt tgcagtgagc
     4741 tgtactcgtt gtgccatata tactgcttct tcgtttatcg ttggggtttt acggctttgc
     4801 cgtttaatat agtgtgctat tgtagctggt cttaaccggg agcaggaaca gagaatctcc
     4861 cgtattacat tttgaggaaa gtcaaaacgt catgacgcgc atgaaatatc tggtggcagc
     4921 cgccacacta agcctgtttt tggcgggttg ctcggggtca aaggaagaag tacctgataa
     4981 tccgccaaat gaaatttacg cgactgcaca acaaaagctg caggacggta actggagaca
     5041 ggcaataacg caactggaag cgttagataa tcgctatccg tttggtccgt attcgcagca
     5101 ggtgcagctg gatctcatct acgcctacta taaaaacgcc gatttgccgt tagcgcaggc
     5161 tgccatcgat cgttttattc gccttaaccc gacccatccg aatatcgatt atgtcatgta
     5221 catgcgtggc ctgaccaata tggcgctgga tgacagtgcg ctgcaagggt tctttggcgt
     5281 tgaccgtagc gatcgagatc ctcaacatgc acgagctgcg tttagtgact tttccaaact
     5341 ggtgcgcggc tatccaaaca gtcagtacac caccgatgcc accaaacgtc tggtattcct
     5401 gaaagatcgt ctggcgaaat atgaatactc cgtggccgag tactatacag aacgtggcgc
     5461 atgggttgcc gtcgttaacc gcgtagaagg catgttgcgc gactacccgg atacccaggc
     5521 tacgcgtgat gcgctgccgc tgatggaaaa tgcataccgt cagatgcaga tgaatgcgca
     5581 agctgaaaaa gtagcgaaaa tcatcgccgc aaacagcagc aatacataac agaaacctga
     5641 aacacaaaac ggcagccctt gagctgccgt ttttttattc tgtcagttgt gaaactgaag
     5701 cgatttagtc actatcgatc tcatcaaata tggctcgctt tgagatattc ctcaagtaaa
     5761 aaaacacctc ttcctgcgat ttctcacaaa aaagattcgt tgacaaaaag tgacaaaatt
     5821 atgagatttc catcacacat tttgacatca ggaacggtat gctgaattca ccaagacggg
     5881 aagacaagag gtaaaattta tgacaatgaa cattaccagc aaacaaatgg aaattactcc
     5941 ggccatccgc caacatgtcg cagaccgtct cgccaaactg gaaaaatggc aaacacatct
     6001 gattaatcca catatcattc tgtccaaaga gccacaaggg tttgttgctg acgccacaat
     6061 caatacacct aacggcgttc tggttgccag tggtaaacat gaagatatgt acaccgcaat
     6121 taacgaattg atcaacaagc tggaacggca gctcaataaa ctgcagcaca aaggcgaagc
     6181 acgtcgtgcc gcaacatcgg tgaaagacgc caacttcgtc gaagaagttg aagaagagta
     6241 gtcctttata ttgagtgtat cgccaacgcg ccttcgggcg cgttttttgt tgacagcgtg
     6301 aaaacagtac gggtactgta ctaaagtcac ttaaggaaac aaacatgaaa catacaccgt
     6361 ttttcttcgc attctttttt accttcccct gaatgggagg cgtttcgtcg tgtgaaacag
     6421 aatgcgaaga cgaacaataa ggcctcccaa atcggggggc cttttttatt gataacaaaa
     6481 aggcaacact atgacatcgg aaaacccgtt actggcgctg cgagagaaaa tcagcgcgct
     6541 ggatgaaaaa ttattagcat tactcgcaga gcggcgcgaa ctggccgtcg aggtgggaaa
     6601 agccaaactg ctctcgcatc gcccggtacg tgatattgat cgtgaacgcg atttactgga
     6661 aagattaatt acgctcggta aagcgcacca tctggacgcc cattacatta ctcgcctgtt
     6721 ccagctcatc attgaagatt ccgtattaac tcagcaggct ttgctccaac aacatctcaa
     6781 taaaattaat ccgcactcag cacgcatcgc ttttctcggc cccaaaggtt cttattccca
     6841 tcttgcggcg cgccagtatg ctgcgcgtca ctttgagcaa ttcattgaaa gtggctgcgc
     6901 caaatttgcc gatattttta atcaggtgga aaccggccag gccgactatg ccgtcgtacc
     6961 gattgaaaat accagctccg gtgccataaa tgacgtttac gatctgctgc aacataccag
     7021 cttgtcgatt gttggcgaga tgacgttaac tatcgaccat tgtttgttag tctccggcac
     7081 gactgattta tccaccatca atacggtcta cagccatccg cagccattcc agcaatgcag
     7141 caaattcctt aatcgttatc cgcactggaa gattgaatat accgaaagta cgtctgcggc
     7201 aatggaaaag gttgcacagg caaaatcacc gcatgttgct gcgttgggaa gcgaagctgg
     7261 cggcactttg tacggtttgc aggtactgga gcggattgaa gcgaatcagc gacaaaactt
     7321 cacccgattt gtggtgttgg cgcgtaaagc cattaacgtg tctgatcagg ttccggcgaa
     7381 aacgacgttg ttaatggcga ccgggcaaca agccggtgcg ctggttgaag cgttgctggt
     7441 actgcgcaac cacaatctga ttatgacccg tctggaatca cgcccgattc acggtaatcc
     7501 atgggaagag atgttttatc tggatattca ggccaatctt gaatcagcgg aaatgcaaaa
     7561 agcattgaaa gagttagggg aaatcacccg ttcaatgaag gtattgggct gttacccaag
     7621 tgagaacgta gtgcctgttg atccaacctg atgaaaaggt gccggatgat gtgaatcatc
     7681 cggcactgga ttattactgg cgattgtcat tcgcctgacg caataacacg cggctttcac
     7741 tctgaaaacg ctgtgcgtaa tcgccgaacc agtgctccac cttgcggaaa ctgtcaataa
     7801 acgcctgctt atcgccctgc tccagcaact caatcgcctc gccgaaacgc ttatagtaac
     7861 gtttgattaa cgccagatta cgctctgacg acataatgat gtcggcataa agctgcggat
     7921 cctgagcaaa cagtcgcccg accatcgcca gctcaaggcg gtaaatcggc gaagagagcg
     7981 ccagaagttg ctcaagctga acattttctt ctgccaggtg cagcccgtaa gcaaaagtag
     8041 caaagtggcg cagtgcctga ataaacgcca tattctgatc gtgctcgaca gcgctaatac
     8101 gatgcaaccg agcgccccag acctgaattt gctccagaaa ccattggtat gcttccggct
     8161 tacgtccatc acaccagacc acaacttgct ttgccaggct accgctgtcc gggccgaaca
     8221 tcgggtgtaa ccccagtacc gggccatcgt gcgccgccag catggcctgt aatggtccat
     8281 ttttcactga tgccagatca accagaatac aatctttcgg taaaggcggt aatttgccga
     8341 taacttgctc agtaacgtgg attggcacac taacaatcac cattccggca tcggcaacaa
     8401 tatcagccgc tcgatcccag tcatgttgct ccagaatccg cacctgataa cccgatagtg
     8461 tcagcatctt ctcgaacagg cgtcccatct gaccgccgcc gccaacgata actaccgggc
     8521 gtaacgcagg acaaagtgtt ttaaatcctt tgtcgttttc actggagtaa gattcacgca
     8581 tcacccgacg caaaacatcc tcaatcagat ctggcggtac acccagagct tccgcctctg
     8641 cacgacgcga ggccaacata gatgcctcgc gctccggaac ataaataggc agtccaaagc
     8701 ggcttttcac ctcgcccact tcagcaacca gttccagacg cttcgctaat aaattcagca
     8761 gcgctttatc gacttcatca atttgatcgc gtaatgcggt caattcagca accataataa
     8821 acctcttaag ccacgcgagc cgtcagctgc ccgttcagat cctgatgaat ttcacgcagc
     8881 aaggcatcgg tcatttccca gctaatgcag gcatcggtta cggatacacc gtatttcatt
     8941 tcactgcgcg gttgctcgga agactgattg ccctcgtgga tattactttc gatcatcaga
     9001 ccaataattg agcgattgcc atctttgatt tgagcaacca cggattctgc caccgcaggc
     9061 tgacggcgat aatctttatt ggaattaccg tggctgcaat ctaccatcag agacgggcgc
     9121 agtcccgcct gttccatctc tttttcacat tgcgcaacat ccgcagggct atagttcggc
     9181 gctttaccac cgcgcaggat cacatgtccg tccggattcc cctgagtttg tagcaacgca
     9241 acctgacctg cctggttaat gccaacaaaa cggtgcggct gggcggcggc gcgcatagcg
     9301 ttaattgctg ttgccagact gccgtcggtg ccgtttttaa aaccaaccgg catggaaagc
     9361 ccggaggcca tttcacggtg agtttgcgat tccgttgtac gagcaccaat tgctgaccag
     9421 ctaaacaaat cgcccaggta ttgcgggcta ttcggatcta acgcttccgt cgccagtggc
     9481 agtcccatat tcaccagctc aagcagcaat ttacgcgcga tctgcagccc ggcttctaca
     9541 tcaaaagagc catccatatg gggatcgtta attaaccctt tccagccgac agtggtacgg
     9601 ggtttttcaa aatagacgcg cattaccaga tagaggctat cgctgacctc tgcggcaagg
     9661 gctttaaatc gacgagcata ttccagagca gtttccggat catgaatgga acaaggacca
     9721 catactacca gcagacgagg atcgcgcccg gcgataatat ctgaaatggt tttacgcgag
     9781 tcagcaatct gggcttcttg ttgcaggctt aatggaaaag cggccttcag ttgtgccgga
     9841 gtcattaaaa cctgttcgtc ggtaatatgt acgttattca gcgcgtcttt ttgcatgatg
     9901 gcgatcctgt ttatgctcgt ttgcgatagt tgatcctcag cgaggatgac gtaacgataa
     9961 cacataaagt aaagttttca atccatattt cgtacatttt tctttacaga agcaatttat
    10021 tcgcgctttc aacccttgat tctgtataga taaatttaca ccacctttga aaacaatccg
    10081 ctatgctttg aaaaaggaga aagaaatgat gaaaaagttt atcgccccct tgttggcttt
    10141 actggttagc ggatgccaga ttgatcctta tactcacgcg ccaaccttga ccagcaccga
    10201 ctggtatgat gttggtatgg aagatgcgat atcgggcagc gccataaaag atgacgatgc
    10261 atttagcgat tcacaggcgg atcgcggtct ataccttaaa ggatatgccg aaggacaaaa
    10321 gaaaacttgc cagaccgatt ttacttatgc ccgaggactt tccggtaaaa gctttcctgc
    10381 cagctgcaac aatgttgaaa atgccagcca actgcatgaa gtatggcaaa aaggggctga
    10441 tgaaaatgcc agcgcaatac gattaaatta attaaaaccc cacaaataaa ttaaacataa
    10501 gattttactc attatgtttt atttctcaca atgatgacga ccgcatgaaa taatgatatt
    10561 ttaatgagca ttctgaaaaa atatcgatgc atttcgagcg aagatggtgg ggatccctga
    10621 atgcgttttt ctcaccgact gttcttactg ctgattctcc tgctgacggg agcaccaata
    10681 ctcgctcagg agccttctga cgttgcgaaa aacgtgcgta tgatggtttc cggtattgtc
    10741 agttacaccc gctggcctgc gctatcaggg ccaccaaagt tatgtatctt ttcatcttca
    10801 cgttttagta ccgccctaca agagaatgcc gcgacatctt tgccttatct gcctgtcatc
    10861 attcacactc aacaagaggc gatgatttca ggctgcaatg gtttttattt tggcaacgag
    10921 tcaccgacat tccagatgga attaaccgaa caatatccgt caaaggcgtt attattaatt
    10981 gccgaacaaa ataccgagtg cattattggt agcgcctttt gtctgatcat ccacaataat
    11041 gacgtcagat ttgccgtaaa cctggatgcc ttatcgcgta gcggtgtaaa ggtcaacccg
    11101 gatgtcttaa tgctcgcacg gaagaaaaat gatggataac gataattctc ttaataagcg
    11161 ccccacgttt aaaagagcat tacgcaacat cagtatgacc agcatattta tcactatgat
    11221 gctgatctgg ttgctgcttt ccgtgacctc ggtgctgacc ctgaaacagt acgcgcaaaa
    11281 aaacctggca ctgacagcag caacaatgac ttacagtctg gaagcagctg tcgtttttgc
    11341 cgatggccct gcagcaactg aaacactggc agcgctgggc cagcaagggc aattttcaac
    11401 tgcagaagta cgtgataagc agcaaaatat tctggcatcc tggcattaca cccgtaagga
    11461 tccaggcgat actttcagca atttcataag ccactggctc ttccctgccc ccatcattca
    11521 gccgattcgt cacaatggtg aaaccattgg cgaagtacgc ttaaccgctc gcgacagttc
    11581 aatcagccat tttatctggt tttcgctcgc cgtactgacc ggttgtattc tgctggcatc
    11641 aggaatcgca attaccctca cccgccattt gcacaatggc ctggtggaag cactgaaaaa
    11701 tatcaccgat gtcgtacatg atgtgcgttc caaccgcaat ttttcccgac gagtttcgga
    11761 agaacgtatc gctgagtttc accgcttcgc tctcgacttc aacagtctgc tggatgaaat
    11821 ggaagagtgg cagcttcgtt tacaggccaa aaatgcgcag cttctgcgta ccgcgctaca
    11881 tgacccatta accgggctgg ctaaccgcgc agcgtttcgt agcggcatca atacgttgat
    11941 gaacaattcc gatgcccgaa aaacgtcggc gttactattt cttgatggcg ataatttcaa
    12001 atatatcaat gatacctggg gtcatgcgac gggcgataga gtcttgattg aaatcgcaaa
    12061 acggttagct gaatttggcg ggctgcgaca taaagcatac cgcctgggcg gcgatgaatt
    12121 cgctatggtg ctctatgatg tacagtcgga atctgaagtg cagcagatat gctcagcact
    12181 gacacaaatc tttaatctcc cgtttgatct tcataatggc catcagacca ccatgacatt
    12241 aagcattggt tacgcaatga ccattgcgca cgcttctgcg gaaaaattac aagagctggc
    12301 cgatcacaat atgtatcagg ccaaacacca gcgtgccgaa aagctggtga gataacaagg
    12361 atatatcgat atgataaagc acctggtagc acccctgatt ttcacctcac taatactgac
    12421 tggctgccag tcccctcagg gaaagtttac tcctgagcaa gtcgccgcta tgcaatctta
    12481 tggatttact gaatccgccg gcgactggtc gctgggctta tcagatgcca ttctgttcgc
    12541 aaaaaatgac tacaaattgc tcccggaaag ccagcaacag atccaaacca tggcagccaa
    12601 attggcctcg acagggctaa cacatgcccg tatggatgga cacaccgata actatggtga
    12661 agacagttac aacgaaggct tatcattgaa acgggcgaat gtcgtggccg atgcatgggc
    12721 tatgggtgga caaattccac gcagcaatct caccacacag ggtttaggaa aaaaatatcc
    12781 catagccagt aacaagaccg cccagggccg cgccgagaac cgccgcgtcg cagtggtgat
    12841 tactacccct taaaacacga gcaaacagat aaaaaaaggg ccagccaatt ggccagccct
    12901 tcttaacagg atgtcgctta agcgaaatct tagttaagac gctctttgat acgagcagcc
    12961 ttaccagtac gctcacgcag gtagtacagt ttagctttac gaacagcacc acgacgtttg
    13021 acagaaatgc tgtcaactac cggagagtga gtctggaaga cacgctcaac gccttcgccg
    13081 ttggaaattt tacgaacagt gaatgcagag tgcagaccgc ggttacgaat agcgataacc
    13141 acgccctcga atgcctgcag acgttttttg gaaccttcaa caacccatac tttcacttcc
    13201 acggtatcac ccggacggaa ggaaggtacg tcctgcttca tctgctcttg ttcaagttgc
    13261 ttaataatgt tgctcataat ttaatctctt atcctgggta aactgatatc tcgggggctt
    13321 acgccatccc atcatgttta tgttgctgtt gtgcgtgttc cgttttgaac tccgccagca
    13381 accttgcttg ctcttcagtc agagccaggt tttccagaag ttcaggtctt ctaagccagg
    13441 tacggcccag cgactgtttc aaacgccagc gacgtatctc ggcatggttg cccgacagta
    13501 acactggcgg aacctccatc ccttctaaca cctcaggccg cgtatagtgc gggcaatcca
    13561 gcaatccttc agcaaaggaa tcttccgttg ccgaggcttc atgtcccagt actcccggaa
    13621 taaaccggga aacggagtca atcagcgtca ttgctggtaa ctcaccacca ctgagaacgt
    13681 aatcgccgat tgaccattct tcgtcaattt cggtttggat cacgcgctca tctatacctt
    13741 cgtagcgacc gcacaccaga atcaattttt gattcgttgc cagttcgctg acgcccgctt
    13801 gatcaagctt gcgtccctgt ggtgaaagat aaatcacctt tgcgccttca cccgccgcgg
    13861 cttttgctgc atgaatggcg tcccgcaagg gttgcaccat cattaacatc cccggtccgc
    13921 cgccgtaagg acgatcgtcc acggtacggt gccggtcatg cgtgaaatcg cgaggactcc
    13981 agctctggat gctcagcagg ccatttttaa ctgcccggcc agttaccccg taatcggtaa
    14041 ttgcgcggaa catttcagga aacaggctaa ttatgccaat ccacatagcg ccgtctttta
    14101 ccgtttatcc ggtggtttaa aaaccaggat cccaatctac ttcgattgaa cgagtagtga
    14161 gatcgacttt cttgataacc tgcccatcga ggaacggtac gagacgttcc ttgataccaa
    14221 acgcatcttt caggtttgcc ttaatgacga gaacgtcatt ggatccggtt tccatcatat
    14281 cgacgacttt accgagatcg tagccttcag tggttactac ctggcagccc atcaggtctt
    14341 tccagtagta gtcaccctct tcaagctgag gcagctgcga tgaatccacg acaatttcac
    14401 aatttgtcag caggttcgcc gcatcacgat cgtcaacgcc tttcagcttg atgatcatgt
    14461 cctgattgtg gtgcttccag ctttccagct ggacttgctg ccactgaccc gccttctgga
    14521 taaaccaggg ctgatagtca aaaatgcttt cggcgtcttc ggtggaagaa aacactctga
    14581 gccacccacg aataccgtaa gacgaaccca tttttcccaa aacgatggga tcaacaggtg
    14641 cttgcgcggt gagttgtttg ctcatcatga ccaccgtgac agattaagct gctttgttta
    14701 cttctttgat cagcgcagca acgcgatcag aaatagttgc gccctggcca acccagtgag
    14761 cgatgcgatc cagatccagg cgagtgcctt cttctttttc gctagcgatt gggttgaaga
    14821 aaccaacgcg ctcgatgaag cgaccgttgc gtgcattacg gctgtcagcg acaacaacct
    14881 ggtagaacgg acgcttttta gcgccgtgac gtgctaaacg aatagttacc ataacatcct
    14941 cttgtgtgaa taaaacaacc ggaccccatc gaggaacgga gtccggtgtc atattaaaag
    15001 cccgaaaatt ttactgattt ctggagaaaa tgcaatcagc agttaataac tctgtcgcat
    15061 aaggtcattt agcgaccagg aaatcccggc ggcatcatcc ccttcatgct tctcatcatc
    15121 ttcgccattc cgcccttctt cattttcttc atcatgcgct gcatgtcgtc gaactgtttc
    15181 agaagacggt taacgtcctg cacctgcata ccgcaaccgg cagcaatacg gcgtttacgc
    15241 gaacctttga tgatttctgg cttagcgcgc tctttcatcg tcatcgagtt gatgatggct
    15301 tccatacgca ccagcacttt atcgtccatc tgtgacttga cgttatccgg gatctgcccc
    15361 atgcccggca gcttgcccat cagactagcc atgccgccca tatttttcat ctggcgcagt
    15421 tgctcaagaa agtcgttgag atcgaagccg tcgccttttt ttagcttgct ggctaatttc
    15481 tctgcctgcg cgcggtcaac tttgctttcg atatcttcga tcagcgacag tacgtcgccc
    15541 atgccgagaa tacgcgacgc gatgcggtcc ggatggaacg gctccagcgc ctcagttttc
    15601 tcgccaacac cgaggaactt gatcggtttg ccagtgatgt gacgaataga gagcgccgca
    15661 ccgccgcggg catcgccgtc cactttggtc aacactacgc cggtcagcgg taacgcttca
    15721 ttgaatgctt ttgccgtatt ggccgcatcc tgaccggtca tggcgtcaac cacaaacagg
    15781 gtttcaaccg gatttatgga cgcatggact tgtttgatct cgtccatcat cgcttcgtca
    15841 acgtgcagac gaccagcggt atccaccagc agcacgtcgt agaatttcag tttggcttct
    15901 ttcagcgccg cgttaacgat atctaccggc ttctgaccaa catcagaagg gaagaaatca
    15961 acgcccacct gctctgcaag cgtctcaagc tgtttgattg ccgctggacg ataaacgtcg
    16021 gcagaaacca ccagcacttt tttcttgtgc ttctcgcgca ggaacttacc gagcttaccg
    16081 acactggtgg ttttaccggc accttgcagg cccgccatca gcacgaccgc aggcggttgt
    16141 gccgccagat tcagggtctg gttctcttcg cccatcgccg caaccagttc gttacggacg
    16201 attttgacga actcctgccc cggcgtcagg cttttattaa cttcatgacc aaccgctttc
    16261 tcttttacgc gattgataaa ctcacgcact accggcagag cgacgtccgc ctccagcagc
    16321 gccatgcgca cttcgcgcag cgtatctttt acgttgtctt cagtgaggcg tccacggcca
    16381 ctgatattgc gcagcgtgcg cgacaaacga tcggttaaat tatcaaacat tgtctctcgc
    16441 ctggggtgga aacggttggc cgcaatcgcg acacatcatc agtattttgc cgcagtataa
    16501 catgaaggcg tctttgttgt tatgcaacgg ttggagcagc gttcacctga cgctatactg
    16561 cttctctttc ttattgctca aactgtcgac attactatgc ccgtttttgc tctgctcgcg
    16621 cttgtcgcct actccgtcag tcttgcgctg attgttcccg gtctgctgca aaaaaacggc
    16681 ggctggcggc gcatggctat tatttctgcg gtcattgcgc tggtctgcca cgcaatcgct
    16741 ctggaagccc gcatcctgcc cgacggtgat agcggacaaa acctcagcct gctgaacgtt
    16801 ggttcattgg tcagtttgat gatctgtacg gtaatgacca ttgtggcttc tcgcaatcgt
    16861 ggctggttgc tgctacccat tgtctatgcc tttgcgctta tcaacctggc gctggcaacc
    16921 ttcatgccca atgaatacat cacccatctg gaagctacac ctgggatgct ggtgcacatt
    16981 ggcttatcgc tcttttccta tgccacgcta attatcgccg ccctgtacgc gctgcaactg
    17041 gcgtggattg attaccaact gaagaacaag aagctggcgt ttaaccagga aatgccgcca
    17101 ttgatgagta tcgagcgtaa aatgttccac atcacgcaga ttggcgtggt gctgctaacg
    17161 ctcacgcttt gcactggcct gttctacatg cacaacttat ttagcatgga aaatatcgac
    17221 aaggctgtgc tctctatcgt ggcgtggttt gtctatattg tgctgctgtg gggacattat
    17281 catgaaggat ggcgtggacg ccgcgtcgtc tggtttaacg ttgcgggtgc agtcattctg
    17341 acactggcct acttcggcag ccgaattgtc cagcagttaa tcagctaaac ccagaaaagg
    17401 agtttcccct ggaacacatt tctactacta cgttgatcat tattctgatc atcatggtgg
    17461 tcatttcagc ctatttttcc gggtccgaaa ccggaatgat gaccctcaac cgctatcgtc
    17521 tgcgacatat ggcgaaacag ggtaatcgct cggccaaacg cgtcgaaaaa ttgctgcgta
    17581 agccagaccg cctgataagc ctggtgttaa tcggcaataa cctggtcaat attcttgcct
    17641 ccgcgctcgg cactattgtt gggatgcgtt tgtacggcga tgcgggcgtg gcaattgcga
    17701 ctggtgtgct gacctttgtg gtgctggttt ttgccgaagt gttgccgaaa actatcgctg
    17761 cgctataccc ggaaaaagtc gcttatccga gtagtattct gttagcaccg ctgcaaattt
    17821 tgatgatgcc gctggtctgg ttgctgaacg ctatcacccg catgttaatg cgcattatgg
    17881 gcatcaaaac cgatatcgtg gtcagcggct ctttgagcaa agaagagttg cgcactatcg
    17941 tgcacgaatc gcgctcacaa atttcccgtc gcaatcagga tatgctgctg tcggtgctcg
    18001 atctggaaaa aatgaccgtt gatgacatca tggtgccgcg cagtgaaatt atcggtattg
    18061 atatcaacga tgactggaaa tctattctgc gccaactctc ccactcacct cacgggcgca
    18121 tcgtgctcta ccgtgattcg ctggacgacg ccatcagtat gctgcgagta cgtgaagcct
    18181 ggcggttgat gtcggagaaa aaagagttca ccaaagaaac catgctgcgc gccgcggacg
    18241 agatctattt tgtgccggaa ggtacgccgc tcagcacgca gttggtaaag tttcagcgca
    18301 acaaaaagaa agtcggcctg gtcgtcaacg agtatggaga cattcaaggg ctggtgacgg
    18361 ttgaagatat tctggaagag attgtcggcg atttcactac gtcgatgtcg ccaacacttg
    18421 ccgaagaggt cacgccgcaa aacgacggtt cggtgattat cgatggcacc gccaacgtgc
    18481 gggaaatcaa caaagccttt aactggcatc taccggaaga tgatgcccgc acggttaatg
    18541 gcgtcattct tgaggcactg gaagagatcc ctgtcgcagg cacccgcgtg cgtattggcg
    18601 agtacgatat agatattctc gacgtacagg acaatatgat taagcaggta aaagtttttc
    18661 ctgtgaaacc gctgcgcgag agtgtggcgg agtaacgaaa acggccctgc attcgcatgc
    18721 agggccgtga attattacga aaacagaaat tacgcttttg ctttcgctac agttaccatc
    18781 gccgcacgaa tcgtacgacc attcagcgta taacccttct gcataatgcc cagtacgtta
    18841 cctggcgcaa cgtcatcaga ttccaccatt gcgatagcct gatgcacatt cgggtccagc
    18901 ggcacgttag tttcggcgat cacttcaaca ccaaacttac gcacaacatc cagcatcgac
    18961 ttcagcgtca gctcaatgcc ttcaaccatc gcagacatat ccgggttagc tttatcagcc
    19021 acttccagcg cacgatccag gctatcaatc accggcagca attcgttgat gaatttctcc
    19081 agcgcgaatt tgtgggcttt ttcaatatcc agttcagtac gacgacgcag gttttccatt
    19141 tcggctttta cacgcaaaat gccgtcacgt tcacgggtct gggcttcagc cagctgagct
    19201 tcgagattcg caattttttc atcgcgcgga tccacctgct cagcagaagc ttctggctca
    19261 actgcctcaa tctcttcgtg ctgatccatg ataatttctt ccggggcttg cccctcaggc
    19321 gttttctgtt ctttactact catgaatttc tccgcgtttt tttcgcattc atctcgctaa
    19381 cttcgcttat tatggggatc agtttcaggg tttcaaggga agcactcaca ttgtcatcaa
    19441 tcttcgcaac aaggacctcg gaaaaatgaa taatcatttc aagtgtattg gcattgtggg
    19501 acacccacgg caccctactg cactgacaac acatgaaatg ctctaccgct ggctgtgcac
    19561 aaaaggttac gaggtcatcg ttgagcaaca aatcgctcac gaattgcaac tgaagaatgt
    19621 gaaaactggc acgctcgcgg agattgggca actggctgat ctcgcggtag tcgttggtgg
    19681 cgacggtaat atgctgggcg cggcgcgcac gctcgcccgt tacgatatta aagttattgg
    19741 aatcaaccgt ggcaacctgg gtttcctgac tgaccttgac cccgataacg cccagcaaca
    19801 attagccgat gtgctggaag gtcactacat cagcgagaaa cgttttttgc tggaagcgca
    19861 agtctgtcag caagattgcc agaaacgcat cagcaccgcg ataaatgaag tggtacttca
    19921 tccaggcaaa gtggcgcata tgatagagtt cgaagtgtat atcgacgaga tctttgcgtt
    19981 ttctcagcga tctgatgggc taattatttc gacgccaaca ggctccaccg cctattccct
    20041 ctctgcgggc ggtcctattc tgaccccctc tctggatgcg attaccctgg tgcccatgtt
    20101 cccgcatacg ttgtcagcac gaccactggt cataaacagc agcagcacga tccgtctgcg
    20161 tttttcgcat cgccgtaacg acctggaaat cagttgcgac agccagatag cactgccgat
    20221 tcaggaaggt gaagatgtcc tgattcgtcg ctgtgattac catctgaatc tgattcatcc
    20281 gaaagattac agttatttca atatattaag caccaagctc ggctggtcaa aaaaattatt
    20341 ctaattttac gccagcctct ttactgtata taaaaccagt ttatactgta cacaataaca
    20401 gtaatggttt ttcatacagg aaaacgacta tgttggcaca actgaccatc agcaactttg
    20461 ctatcgttcg tgagcttgag attgattttc atagcggcat gaccgtaata actggcgaga
    20521 ccggcgcggg taaatctatt gcaatagatg ccctcggtct ttgtctcggt ggtcgcgctg
    20581 aagccgacat ggtgcgtacc ggcgctgctc gcgctgacct gtgcgcccgt ttttctctga
    20641 aagatacgcc agcggccctg cgctggctgg aagaaaacca gcttgaagac gggcatgaat
    20701 gtttgcttcg tcgcgtgatc agcagcgatg gtcgctcccg tggtttcatc aacggtacag
    20761 ctgttcctct gtcacaactg cgcgaactgg gtcagttgct gattcagatc catggtcagc
    20821 acgctcatca gttactcacc aaacccgagc accaaaagtt cctgcttgat ggctatgcca
    20881 atgaaacctc tctactccag gaaatgaccg cacgttatca gttgtggcat caaagctgcc
    20941 gtgacctcgc gcatcatcaa cagttaagtc aggaacgcgc cgcccgtgcg gaactgctgc
    21001 aataccaatt aaaagaactt aacgaattta atccgcagcc cggagagttt gagcaaatcg
    21061 acgaagagta caaacgtctg gcgaacagcg gtcaattgct gaccaccagc cagaatgcat
    21121 tggcattaat ggccgacggt gaagacgcaa acctgcaaag tcagctttac acggctaaac
    21181 aactggtgag cgaattgatt ggcatggaca gcaaactgtc cggcgtactt gatatgctgg
    21241 aagaagctac catccagatc gctgaagcca gcgacgaact gcgccactac tgcgatcgtc
    21301 tggatctcga tcccaaccga ctgtttgaac ttgaacagcg catctcaaaa cagatttcgc
    21361 tggcacgtaa acatcacgtc agccctgagg cattaccaca gtattaccag tcgctactgg
    21421 aagaacagca gcaactggac gatcaggccg actcacaaga aacgctcgcg ctggcggtaa
    21481 cgaaacatca tcagcaggca ctggaaacgg cgcgcgcatt acaccagcaa cgccagcatt
    21541 atgcagaaga acttgcacag ctgatcaccg acagtatgca tgcgctctca atgccgcatg
    21601 ggcagtttac gattgatgtt aaatttgacg agcatcacct gggcgctgac ggtgccgatc
    21661 gtattgagtt tcgggtaacc accaacccag gtcagccaat gcagcctatt gccaaagtcg
    21721 catccggtgg tgaattgtcc cgcatcgcac tggcaatcca ggtcatcacg gcgcgtaaaa
    21781 tggaaacccc ggcactgatt tttgatgaag tggatgtagg gattagcggt ccaacagcgg
    21841 cagttgtcgg caaactgctg cgtcaactcg gcgaatcaac tcaggtgatg tgtgttaccc
    21901 acctgccaca agtcgcggga tgtggtcatc aacactattt tgtcagcaaa gaaaccgatg
    21961 gtgcgatgac agaaacgcat atgcaatctc tggataaaaa agcgcggtta caagagctgg
    22021 cgcgcctgct tggtggcagt gaagtcacac gtaacacact ggcgaatgcg aaagaactgc
    22081 ttgcggcgta aacttttttc ctgcttcacg gtcagagtaa acagcaaaac gccgtaagac
    22141 cggaaagcaa aaggttttaa agtgatgaaa ggtctattat catcggcata ttacagatga
    22201 gccacgtact gctcgggccc gaaaaggaat caaatcacta tgcgctgtaa aacgctgact
    22261 gctgcagcag cagtactatt gatgttgacc gcaggctgtt ccactctgga gcgagtggtt
    22321 taccgtcctg acatcaacca ggggaactat ctgaccgcta acgacgtatc caaaatacgt
    22381 gttggcatga cgcaacaaca agttgcgtac gcattgggta caccgctgat gtccgatcca
    22441 tttggtacga atacctggtt ctatgtcttc cgccagcaac caggtcatga aggtgtaacg
    22501 cagcaaacgc tgacgctaac ctttaacagt agcggtgtgt tgaccaatat tgataacaaa
    22561 cctgcgctga gtggtaacta ataataaagt tgctctcaaa gacgttaaaa aaggtgctca
    22621 atgagcacct tttttctgtc tgttatttat tcgctgattt ttctgctcgt tgcctgcgaa
    22681 gctctttcgg atcggcaatg agagggcgat aaatctccac ccgatcgcca tcatgcacac
    22741 tatcgcttag ttttgccgga cggctgtaaa tgccgacttt gtttttagtt aaatcgatat
    22801 cggtacgcaa ctccagcaag ccactggcgc gaatagcttc ttcaaccgtc gcgccctcct
    22861 gcagcgtcac gcgctgcagg tactgcttct caggtagcgc ataagccacc tcaacggcaa
    22921 ttttacctgg cactgtaaac ctctttcgca cgaaccgtaa aagcctggac catattagac
    22981 gccagctctt taaacacgcg accaaaagcg agttcaatca acttattggt aaactcaaag
    23041 tcgagatgaa actcgatacg acacgcctcc tggctcagcg gcgtaaactt ccatccacca
    23101 atcaatttct tgaacggccc atccaccaga ttcataagaa tactttggtt actggtcaac
    23161 tggttgcggg tagtaaacgt tttgctgatc ccagccttag agacatctac cgccgcagtc
    23221 atctgcccag gagtggactc cagaatccga cttccggtac aacccggcaa aaactgagga
    23281 taagactgaa cgtcattcac taactgatac atttgctccg cgctgtaggg taccagtgcg
    23341 gtccggctaa tctgaggcat aacaatttcc atcaacaaaa atccaacaaa taatatcatt
    23401 tatcctgctg taaaaaaaac gctatcccgg cgctgggtaa catcgggttc atgctaagat
    23461 agagccttgt cccccgcagg attgatatgg ggtgttttcg atttcagatt accgatgatt
    23521 cacgacgctt atgacgaaga aaaaagcaca taaacctggt tcagcgacca tcgcgcttaa
    23581 caagcgcgcc cgtcacgaat actttatcga agaagagttc gaagcgggac ttgccctgca
    23641 aggctgggag gttaaatccc tgcgcgcagg aaaagccaat atcagcgaca gctacgtcct
    23701 tctgcgtgac ggagaggcat ttctgtttgg cgctaacatc acgccaatgg ccgtggcctc
    23761 cacgcatgtg gtgtgcgatc ctacccgtac ccgcaagtta cttctcaacc agcgcgaact
    23821 ggactcattg tacggtcgcg tcaatcgaga aggctatacc gtagtggcgc tctccctgta
    23881 ctggaaaaat gcctggtgca aagtgaaaat cggcgtcgcc aaaggtaaga aacagcacga
    23941 taaacgttca gatatcaaag aacgcgaatg gcaggtggat aaagcgcgta tcatgaaaaa
    24001 cgctcaccgt taaacctgca ctccaattat tgaccagttc ctcaccgcgc ctccctctcc
    24061 ggcggcgcga atgaacatct tattggctat cacatccgac gaaaatgttg ccatcccatt
    24121 gctaaatcga ataaaaatca ggctacatgg tcgctaaatc tttaacgata acgccattga
    24181 ggctggtcat ggcgctcata aatctggtat acttaccttt acacattggg gctgattctg
    24241 gattcgacgg gatttgcgaa acccaaggtg catgccgagg ggcggttggc ctcgtaaaaa
    24301 gccgcaaaaa atagtcgcaa acgacgaaaa ctacgcttta gcagcttaat aacctgctta
    24361 gagccctctc tccctagcct ccgctcttag gacggggatc aagagaggtc aaacccaaaa
    24421 gagatcgcgt ggaggccctg cctggggttg aagcgttaaa acttaatcag gctagtttgt
    24481 tagtggcgtg tccgtccgca gctggcaagc gaatgtaaag actgactaag catgtagtac
    24541 cgaggacgta ggaatttcgg acgcgggttc aactcccgcc agctccacca aatgatgatc
    24601 cggatacgtc cggtgaagta cagaaagccc gcacggcaca agccctgcgg gcttttttgt
    24661 gtctgtcgtt gtccgagaac atccggctaa atccagagaa aattggtaca cgtttaggta
    24721 cacgctatac tgtggtccat taaacgtgta ccaattatgg aagggatcca gacatggcgc
    24781 gcattacacg ccccctcact aacaacgaaa tcctcaaagc gaaaccccgc gaaaaagact
    24841 tcaccctgca tgatggtgat ggcctgttct tactcgtcaa aacctccggt aaaaaactct
    24901 gacgcttccg ttatcaacga ccgggaagca gcagccgcac aaatttgagt ctcggctcat
    24961 accctgccct tacactcgca gcagctcgtc agatacgcga ccagcactta accacgctcg
    25021 cgcaaggcat ggatccacaa cagcaacagg agcaagcgtc agcacaacgc cagattgagt
    25081 tagacagcat tttctcaact gtggccgcta actggttcca gattaaaagc agaagtgtca
    25141 cagaggatta tgcaaaggat atttggcgtt ctttggataa agacgtgttc ccggcaatcg
    25201 gtgcgatgcc cgttcaggat ataaaggcca gaacgattgt tgaagcattg gagccaatca
    25261 aagcacgtgg cgcattagag acggttcgtc gcctagttca gcgtattaac gaaataatga
    25321 tttatgcggt taataccggt ctgatagatg ccaacccggc ttcgggtgta ggaatggctt
    25381 ttgagaaacc caaaaagcaa aatatgccca cgctacggcc agaagaatta cctaagctta
    25441 tgcgttccct ggtcatgtcg aatctgtctg ttccgactcg ctgtcttatt gaatggcagc
    25501 ttctgaccct agtgcgccct tctgaggctt ctggtgctcg gtgggtagag atcgatctca
    25561 atgccaaact ctggacgatt cccgccgaac gaatgaaggc taagcgtgaa cacattgttc
    25621 ctttatcacc acaagcatta gaaattctgg atgtgatgaa acctattagt gcacatcgag
    25681 aacatgtttt tcccagcaga aataatccaa aacaacctat gaatagccaa actgccaatg
    25741 ctgcacttaa gcgaataggt tatggtggga aattggttgc acatggttta cgttcgattg
    25801 caagtacggc aatgaatgaa gccggtttta atgctgatgt aattgaggct gctcttgctc
    25861 attcagatag aaatgaagta cgaaaagcct acaatcgctc aacatattta cacgcaagaa
    25921 ttgaattgat gaattggtgg ggacgacata ttcatcccca ccagtaaact cactctggag
    25981 ttcctaaggc agtaataaaa ccagcgtcaa ggaactccac attatcggat agactatcta
    26041 tcaatgctct tctccaatga ttaagcccat aacgtctggt atagtcatcc ctagttcttt
    26101 ttagaaataa tcttaatcct tcttggcctg cagaattata tgtttcttca agtgaagtat
    26161 agagcccccc taaaaaatca ttcgcttcca tctttaacct ttcatttagg ttaacaactc
    26221 tattttgaaa gcataatgaa tcatatacct ctcgagtcat accctcgatc atattgatat
    26281 taaaatcaat tactaaagca ccatctatca tttcagtatt ggcaacgaaa aatacaccat
    26341 cgggctcatc aaaataatac acatgaataa atccaactcc attttctgct acaccagctc
    26401 tttttttatt attgcactta tgacaggtcg gtatcaggtt tcttgaatat actgcgagac
    26461 atttatatat tgattcggga agataatgat ctaggtcggt tgatgattcg atcccacata
    26521 atgggcattt tttagaactt aataaaatcc ttgagcgaag ggttgaaagc cgtccattta
    26581 tttgcacttc tgaataagct gtttttatag cattttgggt ttcatcactt atttcctctg
    26641 ctaaaaattc agcacaggga gttgctttac gatcgtgata taaagtataa atagtttcaa
    26701 ttttatcctt ttcatcttgt gttaaagcat acttaggatt tccattttta tacgttaaag
    26761 cggttaataa ctcgctcatt ctatccgcat catctggtaa aggtagtgtc cacatatgta
    26821 ttatcctttt accgctaatt tagatattac ataagacctt gcttgtgtac taagac
//
LOCUS       DAZFKF010000067        26598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFKF010000067 DAZFKF010000000
VERSION     DAZFKF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.067"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            330..4142
                     /locus_tag="ACHKA1_003277"
     CDS             330..4142
                     /locus_tag="ACHKA1_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HFO5680885.1"
                     /translation="MAMINLSKEATVGKALTPIAILMMLSFPVASQAAGLVIKNGTVY
                     NANGVPVVDINKPNGSGLSHNIWDNLNVDKNGVVFNNSANESSTSLAGNIQGNSNLTS
                     GSAKVILNEVTSKNPSTINGMMEVAGDKADLIIANPNGITVNGGGSINTGKLTLTTGT
                     PDIQDDKLAGYSVNGGTITLGKLDNASPTEILSRNVVVNGKVSADELNVVAGNNYVNA
                     AGQVTGSVSATGSRNGYSVDVAKLGGMYANKISLVSTEKGVGVRNLGVIAGGVNGVSI
                     DSKGNLLNSNAQIQSASTINLTTNGTLDNTTGTVTSVGTISLNTNKNTIVNTRAGNIS
                     TMGDIYVNSGTIDNTNGKLAAAGMLAVDTNSATLINSGKGSSVGIEAGLVALKTGTLN
                     NSNGQIRGGYVGLESAALNNNNGDIQTTGDIAIISNGNVDNNKGLIRSSTGHIVIGAA
                     GSVNNGSTKTADTGSSDSLGIIADTGVEIGANNINNNGGQIASNGNVSLSSYSTIDDY
                     AGKILSNSKVIIKGSSLRNDTGGISGKQGIEVAVGGSLTNNIGVISSEEGDISLLANS
                     VDNHGGFMMGQNITMESMSGVNNNTALIVASKKLKINAFGNIENRDGNSFGNAYGLYF
                     GMPQQTGGMVGKEGIELSGQNIYNNNSRFIAEDGPLTLQAQNTFDNTRALVTSGADAS
                     IQVGGTYYNNYATTWSAGNLDIDATTLQNSSSGTMIDNNATGFIASDKNLSLEVVNTL
                     TNYGWISGKGDVDVTVNNGNLYNRNTIAAEKGLDIAALNGIENWKDISAGGDLTMNTN
                     RHVTNNSNSNMVGQNIVINAVNDINNRGNIVSDADLNVTTKGNLYNYLYMVGYGDIAL
                     SANSVANNNATIEATGDLIIDSKGNVGNNRGNLHALNGVLSVKGNNLNNDNGEIRGYG
                     DVTLALTGNYDSYKGLLTSETGDVTLTANIVDNAYGLIAGENVSVDAKSTIYNNTALI
                     AANKKLVINAGGNLENRDGNNFLRNNGALFGITDNVGGIVGKEGVTLSAQNVYNNNSS
                     IIAENGPLNLLSRGTLDNTRALLSSGADAIIRAAGTFYNNYATTYSAGNLDVYAASLN
                     NASDGRLEDNTATGVIASDKNLDLSVDNSVTNYGWISGKGDVHFNVLKGTLYNRNAIA
                     ADNALTINALNGVENFKDIVAGTALTIDTQKYVTNNSNSNMLGQTIAINAVNDINNRG
                     NIVGDYSLGVKTTGNIYNYLNMLSYGVAGVSANKVTNSGKDAVLGGFYGLALEANETD
                     NTGTIVGM"
     gene            4231..5850
                     /locus_tag="ACHKA1_003278"
     CDS             4231..5850
                     /locus_tag="ACHKA1_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO5680886.1"
                     /translation="MQLRFNFGLLLILCCFQVAAKDSLDNLIKQQKNADESTLRAHKL
                     EKKDVYSNAQRNAFTLGDLPQEKNCFIINRIELEDNFLGGQLISEVQKAIAGRCVGSA
                     GVQKIAAALQDYYINAGYITTRISIPSQDISKGKLRFQVNAGKIEKTIVEGNDISEWM
                     LPFQRNDILNIRDIEQGLENLQRVPDVDVKINIEPGTRDGYSIVHIDTHRGKNWSVRA
                     SYNNWGDEETGRYQTTAVGYLFNPAKMGDLFYLAGTRSTTGQYENVSSYYSIPVGYWE
                     YGFFYSKSKSQQVIPLSYISLDYVGTSEYLSAKATRTVYRDKTKKFAGSAELIRRKSN
                     YTLNGEELTLQARDMGNIKLGLNYKQQLPGAFWDSTLSWQRFLTWFGGEKTPDMQYGD
                     VSPVSNIFNFEGNYTRQLKNGYYNTAFFAQYAPRELTLQDQITVGDRWSIRGFENSVG
                     LSGNDGFYIKNTLAFPLPGMKANYYAGLDFGQVYQDASYGDESLVGAAVGIDGNIKSL
                     EYNFSVSTPLKYPATLDIDRVNVNFNFSYQM"
     gene            5866..6234
                     /gene="acpS"
                     /locus_tag="ACHKA1_003279"
     CDS             5866..6234
                     /gene="acpS"
                     /locus_tag="ACHKA1_003279"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO5680887.1"
                     /translation="MRIGTDIVEIARITKAIINGEMAFYERVYTAHERLLINPAEPDY
                     ERASGFWAAKEALVKAIGCGFRDGVRFQDIEIKHDAAKAPYFVISGRLQEIFIEQGLQ
                     NNSLSISHCRTHAIATVIVY"
     gene            6248..7009
                     /locus_tag="ACHKA1_003280"
     CDS             6248..7009
                     /locus_tag="ACHKA1_003280"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFO5680888.1"
                     /translation="MNMYKYNDLNEKTVLVTGASGDIGLGICEKYLEQNCDVYALYKS
                     NDVQLTALKASHPAGDKLHILQCDLACPQSVSALCEQIEREAGKIDVLVNNAGIVKDS
                     LFASMSYEDFTQVIETNMFSIFRLTKDALMLLRAAENPAIINVASIAALIPSVGQANY
                     SASKGAILGFTRTLAAEMAPWGVRVNAVAPGMIESKMVKKVSRAVVRAVTSTIPLRRL
                     GKCEEVANTIVFLSSSASSYIVGQTIVIDGGLVMR"
     gene            7013..7561
                     /locus_tag="ACHKA1_003281"
     CDS             7013..7561
                     /locus_tag="ACHKA1_003281"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HFO5680889.1"
                     /translation="MSKYTIPSKIFLEMGGWRQPLLMVDKIADYKYGENGFVSVVKHV
                     TYNEPYLLGHFPEDPIMPGVIISEIFGQASEYLSFLTDICDIWRERFEEELKSLRDIH
                     ARIHRPEMLEIIRTRRSQVRGVLAAQNLKFKDIAYPGDSIDVVSKLAFSDASGFKHYS
                     VTAHVGKKLISQGTIINFRETK"
     gene            7583..7864
                     /locus_tag="ACHKA1_003282"
     CDS             7583..7864
                     /locus_tag="ACHKA1_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309314.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5680890.1"
                     /translation="MSTMNDAIEQRKEVLMTIKTEIIQRLNIQRDEAQIDDDTALFGN
                     GFKLDSVDATEIVVLLDKVFDIKISESDDPSYMRSINSLATFVIQKKND"
     gene            7900..9060
                     /locus_tag="ACHKA1_003283"
     CDS             7900..9060
                     /locus_tag="ACHKA1_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HFO5680891.1"
                     /translation="MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLV
                     DYSHLSIVSVMGDDAWALINQLVSADVSIIRDEQAIYSLVLNEEGTIRGDVYVLCNID
                     GYYLLSEDIPAAELIASMNTILEKAEELDIQSLPEIQDMRENNWGAILLEGPYSWEIM
                     SEIHGFDVIGLPYYEYMNTDEDLLIFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEK
                     YLMQTGGLDYQKIVRLENPCWDASLWEGQSVNPVQLQMQWAVQYDKDDFIGKDAVTEL
                     SQEYTGNKLIGMIAQEECEGIEAGDRVLVEGQDVGYVVKAVFSPALQRFIALTLLEKD
                     YAWSDISGYEIQTAHGIIPAQSKCMPFIYNLSMLVSPTEHSYIDASKNKSAA"
     gene            9134..11692
                     /locus_tag="ACHKA1_003284"
     CDS             9134..11692
                     /locus_tag="ACHKA1_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HFO5680892.1"
                     /translation="MKTNRKRVVITGLGILSSLAENIQDFREALLNKKNGVADSERFA
                     KWFENARAAEVLHDIEYSDLNDDVIDSLDNAALWAYKVGKDALSQAGLMDSRDTLKET
                     GMIVGVSSAGTEAFLPLFEQRMEDFSLRKALFSGAFSSCCSSVSTLLGLQGGVELVAT
                     ACTASPNAVGMAFDYIQNGKSKTMLAVGTEPIYLPTFAGFYALNVMHPDTCTPFSGQS
                     GMSIGEGAGAIVLEEYEHAVARGATIYGEILSYATSCDAFHETGPDPRASGAVQVMNK
                     AMANAGVTPEQIDYVNAHGTGTEANDRIETLAMKKVFANHDKLLVSSTKSYFGHNIGA
                     AGIVELIACLATLPDNRVLPTLNFSQARPNCDLDYVPNEFRDRKVNIFMKNNYAFGGN
                     NCCMILSMEPASIPVSTYDSKRVAISGVGAVSAIGHTLNQVLENIWAQDHHLELGSVT
                     FPYDTLEEAKELLAVLDESNQFAELFDEEYSFSDATLPETASNFKTFQVSGLEPRKHL
                     RRFDQRKATRGGTFALIALTEALEQAKRKIKRDGDELGMIMGMSRGPQETTYKYLQSL
                     KPDPRKVRTSEFPGSLMNAIPTFCGISEGIKGYTTTLATGENSALGALTYGYEIVRQN
                     LQPQVLVGGADEYFPSMSLYMDAVTQKIHMTAEASDYQIYGKDPQGYVPGEGACMLLL
                     EDPQLAAARGAEVLAEVVGYGKSCSNTYFDASLLEEKSASMALAIERALKHAGISAGD
                     IDLVCGTSNGSDDHSRIEIDAIYNTFAAQNPAVPVVNYNACFGFVASAAGLLNLAVIT
                     DCIKKQAVPAIPNTVEFFDERVNFVRQSMKLALNHVLLVGATEGGNYYAFVIKG"
     gene            11697..12920
                     /locus_tag="ACHKA1_003285"
     CDS             11697..12920
                     /locus_tag="ACHKA1_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HFO5680893.1"
                     /translation="MDNAAIISGYSVWMPYAEDCSQLIDNLKQGKRVARTPWFTSNEE
                     AIKCGFKGNPSVATLKQVDDSALDLLSQLIDEALEQAMLDKHCLAGRNVRVYLTGIGP
                     RIDGLDYKSFYNYNDVEDINLTQSITNLHAAKMSQDTISSHLARKYRLQYLPPNMNCT
                     SNSSLTAVHLATQGIEQGGIDLAIVLNCSKIKTQDIWFLETQSMLDSEQVQPFGENSK
                     GVAFAEGFSALLLESAHHRRARQQSEGVRVQTTYTQISAGRSNDASWLSTNVHKVMQA
                     AMKQAEIALDDLAAILPHGNGSAVSDNAEAKAITMFAGERQILVLAYKGQIGYTATGS
                     GVVDLIIGHHSLTHHQLIAPVGNDVIIDSMASLVLTDGSVTNHSKRHLLKVGVGVDGS
                     VIGVVMSNTQAGRAK"
     gene            12917..13870
                     /locus_tag="ACHKA1_003286"
     CDS             12917..13870
                     /locus_tag="ACHKA1_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5680894.1"
                     /translation="MTEKSIYLSSWALTEPDDIFYFYQPTWLNAWEAHMYERGLYGTR
                     ANGWGLKRFGVENDIYAPERIRNPQYMPYLEAMEQVTDSALQQMPTKERMKLMKSRTA
                     FIYADSWGESGPFEKISSALHIATIDTLPKNLLKKFSITEPTCKIRGEKQAFVQALRM
                     AQDYLAWDVFDNVVICAAYRAIPVLVFSEEDVGKPPKRLFPRRADEVNLSVERVGCFI
                     LSQRESAIRLQCGEYHSGTAPQHATDIDVMAFSERRQMPSRPAAGVIDLVAHYGESGC
                     LTPALSMDYLTRHIQPGGKMRTVIADKQFGYHYFDLEYLEG"
     gene            13881..14588
                     /locus_tag="ACHKA1_003287"
     CDS             13881..14588
                     /locus_tag="ACHKA1_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5680895.1"
                     /translation="MQSTIKSLPAISLSNIYKCYGAGAGKVDALKSINLDINKGEFLA
                     LCGPSGSGKSTLLNILSGIDKPTSGTVIFLNKLLNQLPEEQLAVIRGKHLGFIFQFFN
                     LMPVLNVFDNVYFPLVLNGQFSKKEATERTLHYLDSVGLSGFGDRKPGQLSGGQQQRV
                     AIARALAHEPMVVIADEPTGNLDLATGEAILDLLLTINQQTGTTFVISTHSSELKARA
                     RRVVEIQDGVLIHDSDA"
     gene            14572..15228
                     /locus_tag="ACHKA1_003288"
     CDS             14572..15228
                     /locus_tag="ACHKA1_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680896.1"
                     /translation="MTQMHKGFLLIGVFLYAAFVAPKIYAATPDAVKQVLQQGLPAQP
                     AALATLTEALKKEYEARHNVVALVFYAYGLLRQADGYAATNDFIHASEYAKSGFFWLD
                     EAVDLQEKNQRVRYLRARVDAYLPADSGRCVVTVKDTEHMLADPAIWTTTIRDHILAM
                     RYRALRHCKDTTRANALLAQIKGQNAALAQSLTQDFNVVSEWDSEELTQVLLPLMKGE
                     "
     gene            15229..16539
                     /locus_tag="ACHKA1_003289"
     CDS             15229..16539
                     /locus_tag="ACHKA1_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5680897.1"
                     /translation="MSGEILAVNSVLLLILFFVFYQLLFYSRDVKFAFLNLFRHKRRS
                     FSTITAIVLGGVAIFLYGGFIDYSFWILKEQTIRTNIGHVQIYNQHYFETSNKNKSLI
                     ADYAALKKAILSDPTLAADISTLSGQLEFTGVISHYESETSSYFSALGVEPLPALKLG
                     SFDKIIAGSDLSRVKGDEITLGSGLAKTLNAKYDDWLDVMVVNTAGGQGALSLKLRGI
                     FESGIKDYDDVAMKIPLDTAQRMMGTDGVSKVLILLKEDDTAAFSTKLRQFIERNHLP
                     LVVKDWRDVSLFYQQVEGLLSGIYFFIKLIVALIVIFMIGNSMAMNIVERTREITTLR
                     AIGLKPLHVTRLFLTEGIFIGVIGAVGSLLVGGVLAWIINLYGIAMPPSPGQTIGYTA
                     FIKTSNPELIWVTVVLPILTATGASVLPALRASRLNISDAFKFS"
     gene            16548..17336
                     /locus_tag="ACHKA1_003290"
     CDS             16548..17336
                     /locus_tag="ACHKA1_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HFO5680898.1"
                     /translation="MNMAFYGKWFACLWLATSCVQAASTDNKALEIIRRADEIRSPNK
                     PFRYTLTVTEYKAGATQPENKQVLDISMRFMKPQGNEKADARSLVRFIYPPRDKGKIM
                     LSDWYDLWFYTPELRRPMPISRQQRLIGQISNGDVIVTNFEYAYDSTLVGEVTCAEKQ
                     CYKLALVRKSADITWPKVIYYVEKDGDNRPWKAAYYSQDDQLIKEVLYQDFQPVLGKT
                     RPMKITVTDVRHGNNYSVMEYSDVRLESLPEFHFTKEYIQRGAK"
     gene            17333..18721
                     /locus_tag="ACHKA1_003291"
     CDS             17333..18721
                     /locus_tag="ACHKA1_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5680899.1"
                     /translation="MKRTLKISSLLCVALPLTVQADCLSGDEVAQNSDVTLYEQVSYV
                     NKQASAWQIAGKNPYTRHNGYQEAGIGINSGCSIIDNTLDLKLNLYGINEYALKPAGK
                     FETDDSRTRALINRLSLVYSASDSVQFEAGKFAAPSGMFFLRSPSDLQTHYYTGFQST
                     RLHDPKMTSAYQASSWGAKMNLDTRDYAFSASVIPKLATIDKRYVTSGNWSANQQGNS
                     DEAYLLSYSDHRFGEHTPTVNVRLGPSPSLALSDSYHYTPQLTLNVDAAYHRSQQWRH
                     LSHHETAQVEEYQFPDSLYETKDESGVELALGGQYTSDNFSVFGVEYYFQSEGYSHAE
                     QRQQRELIDFLNTTTGYAPLDQAFDSYKYLMASEISNTANQGMLQGKHYLNAWASLPL
                     AGESTLQPSLVVNLIDGSTLLGLHYSTPLSAISNQLEAYAGGYSALGSRYSEFSLFGD
                     TLGLYLGFKYYL"
     gene            18735..19676
                     /locus_tag="ACHKA1_003292"
     CDS             18735..19676
                     /locus_tag="ACHKA1_003292"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO5680900.1"
                     /translation="MNNASQPTVLLFAGQGNPVIGMGSDLWDLNASTKHIWDCASDIS
                     GLNLRRLCQKGPMNRLIQTTVQQLAVTAINVTLYTLCRERLENLTVVGSCGHSVGEYS
                     ALYAAGAISLETLFGMIHFRASLMHDESQRNKGAMLAVKGINREQLQTMITESGIALD
                     ISCDNTPRQQVIGGTQAALNEFATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYL
                     AGLDIASPEHQVLMNVTAKSEVAPSIIKENLSLHLTHTVKWTESLDTFLNMPTSVAFL
                     EISNKPYLGNMLNDFAGVDQQRVMHCRKAFTDAKVFK"
     gene            19673..20659
                     /gene="ycdU"
                     /locus_tag="ACHKA1_003293"
     CDS             19673..20659
                     /gene="ycdU"
                     /locus_tag="ACHKA1_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFO5680901.1"
                     /translation="MIPDYLTFIRFQDKRSLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQRLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVIEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSRRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEVMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(20852..20939)
                     /locus_tag="ACHKA1_003294"
     tRNA            complement(20852..20939)
                     /locus_tag="ACHKA1_003294"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20903..20905),aa:Ser,seq:gga)
     gene            21173..22111
                     /gene="ghrA"
                     /locus_tag="ACHKA1_003295"
     CDS             21173..22111
                     /gene="ghrA"
                     /locus_tag="ACHKA1_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO5680902.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            22166..22903
                     /gene="ycdX"
                     /locus_tag="ACHKA1_003296"
     CDS             22166..22903
                     /gene="ycdX"
                     /locus_tag="ACHKA1_003296"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO5680903.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRALAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            22927..23481
                     /gene="ycdY"
                     /locus_tag="ACHKA1_003297"
     CDS             22927..23481
                     /gene="ycdY"
                     /locus_tag="ACHKA1_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO5680904.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            23583..24074
                     /gene="ycdZ"
                     /locus_tag="ACHKA1_003298"
     CDS             23583..24074
                     /gene="ycdZ"
                     /locus_tag="ACHKA1_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO5680905.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALTPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(24138..24971)
                     /gene="csgG"
                     /locus_tag="ACHKA1_003299"
     CDS             complement(24138..24971)
                     /gene="csgG"
                     /locus_tag="ACHKA1_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO5680906.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(24998..25414)
                     /gene="csgF"
                     /locus_tag="ACHKA1_003300"
     CDS             complement(24998..25414)
                     /gene="csgF"
                     /locus_tag="ACHKA1_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO5680907.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDYGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(25439..25828)
                     /gene="csgE"
                     /locus_tag="ACHKA1_003301"
     CDS             complement(25439..25828)
                     /gene="csgE"
                     /locus_tag="ACHKA1_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO5680908.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            complement(25833..26483)
                     /gene="csgD"
                     /locus_tag="ACHKA1_003302"
     CDS             complement(25833..26483)
                     /gene="csgD"
                     /locus_tag="ACHKA1_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO5680909.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQEHVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         6834 a         5993 c         6882 g         6889 t
ORIGIN      
        1 accttgttgt tttattcgaa tatctgaaat gattctgtac agaaaactac atatcatttc
       61 agatattcgc tgaattggat tttttctcac ccagggagaa ggggtgggga cttttaaatt
      121 attgtgtatt agtaagttag gtacgtctcc gttcgaagaa aaaacaataa ctcttagttg
      181 ttttttgagt ccttatttca gcttattttt cgtgattttt tattgcgcca ggtgatagga
      241 tttgccgcaa tcatttttgt tgatattccg caaatcatca aaagtgatct gtatgtccaa
      301 aatgaatagc atcaataagg aaaacgttag tggcgatgat aaatttaagt aaggaagcaa
      361 cggtgggtaa agcattaacc cctattgcta tacttatgat gttgtctttt cctgtagctt
      421 ctcaagcggc gggattagtc ataaaaaatg gaacggtata taacgccaat ggtgtgccag
      481 tcgttgacat caacaaacct aacggtagcg gtttatctca taatatctgg gataacctaa
      541 acgttgataa aaatggtgtc gttttcaata acagcgctaa tgaatccagt acttcacttg
      601 ccggaaatat tcagggaaac agtaatctga cctccgggtc ggcgaaggtg atcctgaatg
      661 aggttacttc caaaaatcct tcaaccatta atgggatgat ggaagttgca ggggataaag
      721 cggatctgat tattgccaac ccgaatggta ttactgtaaa cggtggcggt tcaatcaata
      781 caggtaaact gaccttaacc accgggacgc cggatatcca ggatgacaag ctggccggtt
      841 actccgtgaa cggcggtacc attacgctcg gtaaactgga taacgccagc ccgacagaaa
      901 ttctgtcccg taacgtggtg gttaacggca aagtgtctgc cgatgagctg aacgttgttg
      961 ctggcaataa ctatgttaat gccgcaggcc aggtgaccgg tagcgtatcc gccacgggtt
     1021 cccgtaacgg ttacagcgta gatgttgcca aactgggcgg aatgtatgcg aacaaaatca
     1081 gtctggtcag caccgagaaa ggtgttgggg ttcgcaacct cggcgttatt gctggggggg
     1141 ttaatggtgt cagcatcgat tccaaaggta acctgttaaa cagtaacgcc cagattcagt
     1201 ctgcaagcac gatcaacctg acaacaaatg gtactctgga taacaccacc ggtacggtga
     1261 catctgtagg cactatctcg ctcaatacca acaagaatac tatcgtgaat acccgtgcgg
     1321 gtaacatctc tacgatgggt gatatctacg ttaacagtgg cacgattgat aacaccaacg
     1381 gcaagctcgc cgcagcaggt atgctggcgg tcgataccaa tagcgccacg ctgattaact
     1441 caggtaaagg gagttctgtc gggattgaag cggggctcgt ggcactgaaa accggaacgc
     1501 tcaacaacag caatggtcag attcgcggtg gctatgtggg tcttgaatcc gctgcgctga
     1561 ataacaacaa cggtgatatc cagaccaccg gcgatatcgc cattatcagt aacggtaatg
     1621 tggataacaa caaaggtctg atccgttcgt ccaccgggca tatcgttatt ggcgcggcag
     1681 gtagcgtaaa taatggttca accaaaaccg ccgataccgg cagttctgac tctctgggca
     1741 ttattgcaga taccggcgta gaaattggtg cgaacaacat caataacaac ggcggacaga
     1801 ttgcgtctaa tggcaacgtc tccctgtcaa gttacagcac gatcgacgac tatgcgggca
     1861 aaattctgtc caacagcaaa gtgattatca agggaagctc actacgtaac gataccgggg
     1921 ggatcagcgg taagcagggc attgaagtcg ccgttggcgg cagcctgacc aataacattg
     1981 gcgtgatcag ctctgaagag ggtgatatct ccctgttagc caactcagtg gacaaccacg
     2041 gcggcttcat gatggggcag aacatcacga tggagtcgat gtctggcgtc aataacaaca
     2101 ccgcgctgat cgtggccagc aaaaaactga agataaacgc gttcggtaat attgaaaacc
     2161 gcgatggcaa tagcttcggt aatgcatatg gtctgtactt cggcatgcct cagcaaacgg
     2221 gtggaatggt cggcaaggaa ggcatcgagc tttccgggca gaatatctat aacaacaaca
     2281 gtcgtttcat cgctgaggat ggtcctctga ctctgcaggc acagaatacg ttcgacaaca
     2341 cgcgtgctct ggtcaccagc ggggcggatg catctattca ggttggcgga acgtattaca
     2401 acaactacgc taccacctgg agcgcgggta acctggatat cgacgcgacc acactgcaaa
     2461 acagcagtag cggtacgatg atcgataaca atgcgaccgg gttcatagca tctgataaaa
     2521 acctgtcact ggaagtggtg aataccctta ccaactatgg ctggatcagc ggtaaaggtg
     2581 atgttgatgt cacggtgaat aacggcaacc tgtacaaccg caatactatt gcggctgaaa
     2641 aggggctgga cattgcagcg ctgaacggta ttgaaaactg gaaggatatt tctgctggcg
     2701 gcgacctgac gatgaacacc aatcgccatg tgaccaacaa ctccaacagc aatatggtgg
     2761 ggcagaatat cgttattaac gcggttaacg atatcaacaa ccgtggcaac attgtcagtg
     2821 acgctgactt gaacgtgacg accaaaggca acctgtataa ctatctctat atggtagggt
     2881 atggggatat cgcattgtcg gcaaatagcg tggcgaacaa taacgcgacc atcgaagcga
     2941 caggcgatct gattatcgat tcgaagggta acgtgggtaa caaccgcggt aatctgcatg
     3001 cgttgaacgg cgtgttgtct gttaaaggca acaatctgaa caacgataac ggtgaaattc
     3061 gtggttatgg cgatgtcacg ctggcactga cgggcaacta cgacagctat aagggtttgc
     3121 tgacctctga aacgggtgac gtgactctga cggcgaacat cgtagacaac gcctatggtt
     3181 tgattgccgg tgagaatgtt tctgtcgatg ctaaatcgac gatttacaac aacactgcgc
     3241 tgatcgcggc gaataaaaag ctggttatta acgctggcgg caacctcgaa aaccgcgacg
     3301 ggaataactt cctgcgtaat aacggcgcgc tgtttgggat caccgacaac gttggcggca
     3361 tcgtaggtaa agaaggtgtc acgctttctg ctcagaacgt ctacaacaat aacagcagca
     3421 tcatcgctga aaatggtccg cttaatctgc tgtccagggg aacgctggat aatacccgcg
     3481 cgcttcttag cagtggggct gatgccatca tccgtgcggc agggacgttc tacaacaact
     3541 atgccaccac gtacagcgcc ggtaatctcg acgtttatgc ggcgtcgttg aacaacgcca
     3601 gcgatggtcg cctggaagac aataccgcca cgggcgtgat tgcgtctgac aaaaacctgg
     3661 atctgagcgt tgataacagc gtcactaact atggttggat cagcggtaaa ggagatgtgc
     3721 atttcaatgt tctgaaaggc acgctgtata accgtaatgc catcgcggcg gacaacgcgc
     3781 tgaccattaa tgccctgaac ggtgttgaga actttaaaga cattgtggcg ggtactgcgc
     3841 tgactattga tacgcagaag tatgttacca acaacagcaa cagtaatatg ttgggacaaa
     3901 ccatcgcgat caatgccgtg aatgacatta ataaccgcgg aaatattgtg ggtgattatt
     3961 ctctgggtgt taaaaccacc ggtaatattt ataactacct caatatgctg agttatggtg
     4021 tcgctggcgt atcggcaaat aaggttacga atagcggtaa agacgccgtt ctcggtggct
     4081 tctacggttt agcgttagaa gcaaacgaaa ctgataacac cggtactatt gtcggcatgt
     4141 aagatgtaac caaagacagc agtggctttt aatattaatg cgtcatgtca cgcaggggtt
     4201 gctgctgtct ttatttagtt tgagaagata atgcaattac gatttaattt tggtttgctg
     4261 ctcattcttt gttgttttca ggttgcagcg aaagacagtc ttgataattt gatcaagcag
     4321 caaaaaaatg cagatgaaag cacgttgcgc gcgcataaat tagaaaaaaa agatgtttac
     4381 tcaaatgcac aacgtaatgc gtttactctg ggtgatctgc cgcaggaaaa gaattgcttt
     4441 atcatcaatc ggattgaact ggaagataac tttctgggtg gtcagttgat ttctgaagtg
     4501 caaaaagcga ttgccggacg ttgtgtcggt agtgctggtg tgcaaaaaat tgctgctgcc
     4561 ttgcaggatt attatattaa tgccggatat atcacaacgc gaattagtat cccttcgcag
     4621 gatatttcta aaggcaagct acgtttccag gttaatgccg ggaagataga aaaaacgatt
     4681 gtcgaaggta atgatatcag tgaatggatg ttaccttttc agcgtaacga tattctcaat
     4741 attcgtgata ttgaacaggg gctggaaaat cttcagcgag tacctgacgt tgatgtgaaa
     4801 attaatattg aaccgggaac acgggatggt tacagcatcg ttcatattga tacacaccgg
     4861 gggaaaaact ggagcgttcg ggcaagttac aacaattggg gcgatgaaga aacgggccgt
     4921 taccagacaa ccgcggtggg ttatttgttt aatccagcga aaatggggga tctgttttat
     4981 ctcgccggta cgcgtagtac taccggacag tatgaaaacg tcagtagtta ttattccatt
     5041 ccggtggggt actgggagta cggctttttc tatagcaaaa gtaaatcgca gcaggtgatt
     5101 ccgctttcgt acatctcgct ggattatgtt ggcaccagcg aatacttgag cgcaaaagcg
     5161 acgcgtaccg tttatcgcga taaaacaaaa aaattcgccg gtagtgcaga acttattcgt
     5221 cgaaagtcga attacacgct taatggtgag gagctgacat tacaggcgcg ggatatggga
     5281 aatatcaagc tggggctaaa ttataagcaa cagttacccg gcgcgttctg ggattcaacc
     5341 ctctcgtggc aacgcttttt aacctggttc ggtggtgaaa aaacgccgga tatgcaatat
     5401 ggcgacgtta gcccggtgag caatatcttt aattttgagg gtaactatac ccgacaacta
     5461 aagaatgggt attacaatac tgcttttttt gcccagtacg caccgcgtga actgactttg
     5521 caagatcaaa ttacagttgg cgatcgttgg agtattcgcg ggtttgaaaa tagcgtaggt
     5581 ttgtcaggga atgatggttt ttatataaaa aatacgctgg catttccttt gcctggaatg
     5641 aaagctaact attatgccgg tcttgatttt gggcaagtgt atcaggacgc ttcttatggc
     5701 gatgaatcac tggtgggcgc ggcagttggt attgatggaa atattaaaag tcttgagtat
     5761 aacttttccg tttctacccc attaaagtat ccggcgacat tagatattga tcgggtgaat
     5821 gtaaatttta atttttcgta ccaaatgtaa aggataacgg gactcatgag aatagggaca
     5881 gatattgttg aaattgccag aataaccaaa gccattatta atggagagat ggctttttat
     5941 gagcgtgttt atactgctca tgagcgctta ctcattaacc ctgcagaacc tgattatgaa
     6001 cgggcctctg gattctgggc ggcgaaagaa gcgttagtta aagcgattgg ttgtggtttt
     6061 cgtgatggcg tacgtttcca ggatattgaa ataaagcatg atgcagccaa agcaccttat
     6121 ttcgttattt ccggaagact acaagaaatt tttatcgagc aggggttgca aaataactcc
     6181 ctgagtattt cgcattgccg tacccacgcc attgcgactg tcatcgttta ttaactcaac
     6241 atatgtgatg aatatgtaca aatataatga tttaaatgaa aaaaccgtac tggtgacagg
     6301 agccagtggt gatattggac tgggtatttg cgaaaaatat ctggagcaga actgtgatgt
     6361 ttatgcgctc tataaatcca acgacgtgca attaacggcg ctgaaagcaa gccatcctgc
     6421 aggcgataaa ctgcatattt tgcagtgcga tctcgcctgc ccccagagcg tgagcgcatt
     6481 gtgcgaacag atagagcgcg aagctggcaa gattgatgtg ctggtgaata acgcgggcat
     6541 cgttaaagac agcctgtttg cctcgatgag ctatgaggat ttcacccagg ttatcgaaac
     6601 caacatgttc agcattttcc gtctgacgaa agacgcgctg atgttgcttc gtgcggcaga
     6661 aaatcccgcc attattaacg ttgcctctat tgcagcgctg atcccaagcg tcggtcaggc
     6721 gaattacagc gcgtcaaaag gggcgatcct aggcttcacc cgcacgctgg cagcagaaat
     6781 ggcaccctgg ggcgtgcgcg tcaacgcggt cgcaccggga atgattgaat cgaaaatggt
     6841 gaaaaaagta tcccgcgcag tggtccgtgc ggtaaccagt accatcccgt tacgccgtct
     6901 ggggaaatgt gaagaagtgg cgaacaccat cgtcttctta agttcctctg cctccagcta
     6961 cattgtcggc cagaccatcg ttatcgatgg cggcctggtc atgcggtgag ttatgtctaa
     7021 atacacgatt ccttcgaaaa tcttcctcga aatgggcggc tggcgtcagc cgctgctgat
     7081 ggtcgataaa attgcagatt ataaatacgg tgaaaatggc tttgtcagtg tcgtgaaaca
     7141 cgtcacctac aacgaaccgt atctgctggg gcatttcccg gaagatccga taatgcccgg
     7201 ggtaattatt tcggaaatct tcggccaggc cagcgagtat ctctctttcc tgaccgacat
     7261 ttgcgacatc tggcgcgaac gttttgaaga agagctgaag tcgttacgtg atattcacgc
     7321 acgcattcac cgccctgaaa tgctggagat tatccgtacc cggcgcagcc aggtacgcgg
     7381 cgttctggcg gctcagaacc tgaaatttaa agatatcgcc tatccgggtg attctattga
     7441 tgttgtcagc aaattggctt tttcggatgc cagtggtttt aagcactatt ccgtgaccgc
     7501 gcatgtcggt aaaaagctga ttagtcaggg caccattatt aatttccgtg aaaccaagta
     7561 atccaataag agagtaaaca ctatgtctac catgaatgac gctattgaac agcgtaaaga
     7621 agttctgatg accattaaaa ctgagatcat ccagcgactg aatattcaac gcgatgaagc
     7681 gcaaattgat gatgatactg cgctgttcgg taatggtttt aaactggact ccgtcgatgc
     7741 cacggaaatt gtcgtcctgc tggataaagt ttttgatatc aagatcagcg aaagtgatga
     7801 cccgtcatat atgcgcagta tcaatagtct ggccaccttc gttattcaaa agaaaaacga
     7861 ctaactactt tttaaagaat taaatatcag gacacgttta tgaaatctct tgctgaattt
     7921 cacttgaaaa ataatgcggt aatgggtgtg tataacaacc gtaccctacc ttcttcttat
     7981 cacgatgcta tgactgaata taaagctgta cgcgaaaacg cgctgctggt ggactattct
     8041 cacctctcta tcgttagcgt catgggcgat gatgcctggg cgctgatcaa ccagctggtt
     8101 tccgcagatg tctccatcat tcgtgatgag caggcaatct actcactggt gttgaacgaa
     8161 gagggcacca ttcgcggaga cgtctatgtg ctgtgcaaca tcgacggtta ttacctgctg
     8221 tctgaagata ttccggctgc cgaacttatc gccagtatga ataccattct cgaaaaagcg
     8281 gaagaactgg atattcaatc gctgcctgaa attcaggata tgcgggaaaa caactggggg
     8341 gcaatcctgc tcgaaggccc atactcctgg gagatcatgt ctgaaattca tggctttgac
     8401 gtgattggcc tgccttacta cgaatacatg aataccgatg aggatctgct gatcttccgc
     8461 tgtggtaaac acggtgagta tgcgtacatg accatcggtg agcaggcgaa actggcggag
     8521 cagtgggaaa aactgttaac cgtcggtgag aaatacctga tgcagaccgg cggtctggat
     8581 tatcaaaaaa tcgtacgcct cgaaaatccg tgctgggatg ccagcctgtg ggaaggtcag
     8641 tcggtgaatc cggttcagtt gcagatgcag tgggcggtgc agtacgacaa agatgatttt
     8701 attggcaagg acgcggtgac agagctctcc caggagtata ccggtaataa actcatcggc
     8761 atgatcgctc aggaagaatg cgaaggcatt gaggctggcg atcgtgtgct ggtggaaggt
     8821 caagacgtgg gctatgtcgt gaaagcggta ttttccccgg cattgcagcg tttcattgcc
     8881 ctgaccctgc tggaaaagga ttacgcctgg tctgatatca gcggctacga aattcaaact
     8941 gcccacggaa ttattccggc gcaatcaaaa tgtatgccgt ttatttataa cctgagcatg
     9001 ttggttagcc caactgagca cagttatatc gacgcgtcaa aaaataaaag cgcagcgtaa
     9061 tgaatatcgc cgcgtgacta tagcttacgc ggcttaaatt aaccccaatt ggcgggattg
     9121 ccaggtatta ttcatgaaaa cgaacaggaa aagagttgtt attaccgggc tggggatttt
     9181 gtcctcactg gcggaaaata ttcaggattt ccgggaagca ttattaaata aaaaaaatgg
     9241 cgttgcggat agcgaacgct ttgcaaaatg gtttgaaaat gcgcgtgcgg cagaagtgtt
     9301 gcatgacatt gaatactccg atctgaacga tgatgttatc gattcactgg ataacgcagc
     9361 gctctgggcc tacaaagtgg gtaaagatgc gctgagtcag gccggcctga tggatagccg
     9421 tgacaccctc aaagaaacag gcatgatcgt cggtgtttcc tccgcgggta cagaggcctt
     9481 tttaccgctg ttcgaacagc gcatggaaga tttctcgctg cgtaaagcac tcttttccgg
     9541 cgcattctca tcctgttgtt ccagcgtttc aacgctgctg ggcttacagg ggggggttga
     9601 actggtcgcg accgcgtgta ccgccagccc gaacgccgtg gggatggcct ttgactacat
     9661 ccagaatggt aaaagcaaaa ccatgctggc agtaggtacc gagcccatct atctgccgac
     9721 ctttgccgga ttctacgcac tgaatgtcat gcatccagac acttgtacgc cgttctcggg
     9781 tcagtccggt atgtcgattg gtgaaggtgc gggcgctatc gtactggaag agtatgagca
     9841 tgcagtagcc cgtggggcga ccatttatgg tgagatcctc tcttacgcca cttcctgcga
     9901 tgctttccat gagaccgggc cagatccgcg tgccagcggt gcggtgcagg tcatgaacaa
     9961 ggcaatggca aatgcgggcg tcacaccgga gcaaatcgac tacgtcaacg cgcacggtac
    10021 gggtactgaa gccaatgacc gtatcgaaac gctggcaatg aaaaaggtct ttgctaacca
    10081 cgataaactg ctggtgagct ccaccaaatc ttactttggc cacaacattg gcgcagcggg
    10141 gattgtggaa ctgattgcct gtctggcaac actgccggac aaccgcgtac tgccgacgct
    10201 gaatttcagc caggcacgac cgaattgcga tctggactat gtgccgaatg aatttcgcga
    10261 tcgcaaagtg aacatcttca tgaagaataa ctatgccttc ggcggcaaca actgctgcat
    10321 gatcctgagc atggagcccg cgtccattcc ggtcagtacc tatgattcga agcgtgtggc
    10381 tatctcgggc gtcggtgcag tatccgcaat tggccacacg ctcaaccagg ttctggaaaa
    10441 catctgggcg caggaccacc accttgaact gggtagcgta accttcccgt acgatacact
    10501 ggaagaagcc aaagagctgc tggccgtgct ggatgaatca aaccagtttg ccgagctgtt
    10561 tgatgaagag tacagcttca gcgacgctac gcttccggaa acggcaagca acttcaaaac
    10621 gttccaggtt tccgggctgg agccgcgtaa acacctgcgt cgtttcgatc agcgtaaagc
    10681 aacccgtggc ggcacgtttg ccctgatcgc actcaccgaa gcgctggaac aggcgaagcg
    10741 taaaatcaaa cgcgacggcg atgagctggg catgatcatg gggatgtccc gcggcccgca
    10801 ggaaaccacc tacaaatatc tgcaaagcct gaaacccgat ccacgcaaag tacgtacgtc
    10861 ggaattccct ggctcactga tgaatgcgat cccaacgttc tgcgggatct ctgaagggat
    10921 caaagggtat accactacgc ttgcgacagg ggaaaactcg gcgctgggcg cactgaccta
    10981 cggttatgaa atcgtccggc agaacctgca accgcaggta ttggttggcg gcgcggatga
    11041 gtacttcccg tcgatgtcgc tgtatatgga tgcggtgaca caaaagatcc acatgacggc
    11101 tgaggcgagc gactatcaaa tttacggtaa agatccgcag ggttatgttc cgggcgaggg
    11161 tgcctgcatg ctgctgctgg aagatccaca gttggctgcg gcgcgtggcg ctgaggttct
    11221 cgcggaagtg gtgggctacg gtaagtcatg tagtaatacc tacttcgacg cctcattgtt
    11281 ggaagaaaaa tccgcgtcga tggcgctggc cattgaacgc gcccttaagc atgcgggcat
    11341 ctctgctggc gatattgatt tggtatgtgg taccagcaac ggcagcgatg accattctcg
    11401 tatcgaaatt gacgccattt acaacacctt cgccgcgcaa aacccggcgg tgccggtagt
    11461 gaactacaac gcttgctttg gttttgtcgc ctctgccgct ggtttgctta acctcgccgt
    11521 gattactgac tgcattaaga agcaggcagt acctgcgatt ccaaatacgg tggaattctt
    11581 tgatgagcgt gtgaattttg tacgccagtc gatgaaactt gcgctgaacc atgtcctgct
    11641 tgttggcgca acggaaggcg gcaactacta cgcgttcgtc attaagggat aagactgtgg
    11701 acaatgccgc tattatttcg ggttacagcg tgtggatgcc gtatgccgaa gactgttcgc
    11761 agttaattga caatctgaaa caggggaaac gcgttgcgcg tacaccctgg tttacctcca
    11821 acgaggaagc cattaaatgc gggtttaagg gtaatccgtc agtggcgacg ttgaaacagg
    11881 tggacgatag cgcattggac ctgctgtctc agttgatcga cgaagccctg gaacaggcaa
    11941 tgctggataa gcattgcctg gccgggcgca acgttcgcgt ttacctgacc ggtattgggc
    12001 cgcgtatcga cgggctggat tacaaatctt tctataatta caacgatgta gaagatatca
    12061 acttaacgca atccatcacc aatctgcatg ccgcgaagat gtcgcaggac acgatttcaa
    12121 gccatcttgc gcgcaaatat cgcttgcagt atttgccgcc taacatgaac tgcaccagta
    12181 actcgtcact gactgcggtg catctggcga cgcagggcat tgagcagggc gggattgatc
    12241 tcgcgattgt gctgaactgt tcgaaaatta aaacccagga tatctggttc ctggaaactc
    12301 aatcgatgct ggacagcgaa caggttcagc cgtttggtga aaacagtaaa ggtgtggcgt
    12361 tcgcagaagg ttttagcgcg ctcctactgg aaagcgctca tcaccgccgg gcgcgtcaac
    12421 aaagtgaggg cgtaagggtg caaacgactt acacccaaat cagcgcgggc cgcagcaacg
    12481 atgcctcctg gcttagcact aacgtgcaca aggtcatgca ggccgcgatg aagcaggctg
    12541 aaattgcgct ggatgatctt gcagccattc ttccacacgg caacggttcg gcagtgagcg
    12601 ataacgcgga agccaaagcc atcaccatgt tcgcagggga gcggcagatc ctcgttctcg
    12661 cctataaagg gcagattggc tataccgcaa ccggatctgg tgttgtcgat ctgatcattg
    12721 gccaccattc gttgacgcat catcaactga tcgcacctgt tggtaacgac gtcattatcg
    12781 acagcatggc ttcgctggta cttacggacg gcagcgtgac aaaccatagc aaacgccatt
    12841 tgctgaaggt tggtgtgggt gtcgatggtt cagttattgg cgttgtcatg tcaaatacgc
    12901 aagcggggcg cgcgaaatga cggaaaagag tatctacctg tcttcctggg cgcttacgga
    12961 accggacgac attttttatt tttatcagcc gacctggctc aacgcctggg aagcacatat
    13021 gtacgaaaga ggattatatg gcactcgtgc taatggctgg gggctgaagc gctttggcgt
    13081 tgagaacgat atttatgcgc cggaacggat ccgtaatcca caatatatgc cttaccttga
    13141 ggcgatggag caggtaaccg attctgcatt acaacaaatg ccgactaaag agcgcatgaa
    13201 gttaatgaaa tcgcgcacgg catttattta tgccgattcc tggggggaat ccggtccgtt
    13261 tgaaaaaatt tccagtgcgc tgcatatcgc cactattgat acgcttccca agaatttact
    13321 taagaagttt tcgataacgg aaccgacttg caagattcgc ggcgaaaaac aggcattcgt
    13381 tcaggcgctg cgtatggcgc aggattatct cgcctgggat gtattcgata acgtggtaat
    13441 ttgtgctgcc tatcgggcaa ttccggtgct ggtattctct gaagaagatg tgggtaaacc
    13501 gcctaagcgt ttatttcccc gtcgtgctga tgaggtgaat ttatctgttg aacgagtggg
    13561 ttgttttatt ctcagccagc gtgagagcgc tatcaggctg caatgcgggg aatatcattc
    13621 cggcactgcg ccacagcatg ctacggatat tgatgtcatg gccttcagcg aacggcgaca
    13681 aatgccgtcc cggcctgctg ctggcgttat cgacctggta gcccattacg gagaaagcgg
    13741 ttgtctgacg cccgcgctga gtatggatta tttaacccgt catattcagc ccggtggaaa
    13801 aatgcgtacg gtcattgccg ataaacagtt tggttatcac tactttgacc ttgaatacct
    13861 tgaaggataa cgacagtcgt atgcaatcta ctattaaatc gttgcccgct atttcgctta
    13921 gcaacattta caagtgttat ggcgctgggg caggaaaggt tgatgcgctt aaaagcatta
    13981 atcttgatat caataaaggc gaattcctcg cactgtgtgg gccgtcaggc agtggtaaaa
    14041 gtactctgtt gaatattctc tcgggtattg ataaaccgac cagcggaacg gtgatatttt
    14101 taaataaatt actgaaccaa ctgccagaag agcagttggc tgtgattcgt ggaaaacatt
    14161 tgggttttat ttttcagttt tttaacctta tgccggtatt aaatgttttc gataacgttt
    14221 atttcccact ggtgcttaat ggtcagttca gtaaaaaaga agccactgaa cggacgctgc
    14281 attatctcga tagtgttgga ttaagcggtt ttggcgatcg taaacctggg caactatcgg
    14341 gcggtcaaca acagcgtgtc gccatcgcca gggcactggc ccacgaaccg atggtggtca
    14401 ttgccgatga accgacggga aatttagacc tggcaaccgg cgaggctatt ttggacctgt
    14461 tgttgacgat taatcagcag accggcacca catttgttat ttccacgcac tccagcgaat
    14521 taaaagcgcg ggcacggcga gtggttgaaa ttcaggatgg agtattgatt catgactcag
    14581 atgcataaag gatttttgct catcggcgtc tttttgtacg cggcgtttgt ggctccgaag
    14641 atttacgcgg caacccccga cgctgttaaa caggttctgc aacaggggct cccggcgcag
    14701 cctgccgcgc tggcgacgct gaccgaggcg ttaaagaaag agtatgaagc caggcacaat
    14761 gtggtggcgc tggtttttta cgcctatggc ttactgcgtc aagcggacgg ttatgcggca
    14821 acaaacgatt ttatccatgc ctcagaatac gccaaaagcg gctttttctg gctggatgaa
    14881 gcggtggatt tgcaggagaa aaatcaacgc gtacggtatt tacgcgcccg cgtagatgcc
    14941 tatttacccg cagactcagg gcgttgcgtg gtgacagtca aagacaccga acacatgctg
    15001 gctgacccgg cgatatggac gacaacgatc cgcgaccata ttctggcgat gcgttatcgc
    15061 gcattacgtc actgcaagga caccacccgc gcgaatgcgt tgttggcgca aattaaaggg
    15121 caaaacgccg cgcttgcgca gtcgctgacg caggatttta atgttgtatc agaatgggat
    15181 agtgaagagt taacccaggt actgctgccg ctgatgaaag gtgagtaaat gtccggggaa
    15241 attttagctg ttaatagcgt tctgctgctg atcctgtttt ttgtgtttta tcagcttctg
    15301 ttttattcac gagatgtcaa atttgcattt ttgaatctct ttcgccacaa acgtcgctct
    15361 ttttctacca taaccgcaat tgtattgggt ggtgtggcga tctttttata tggcggattt
    15421 attgattact ctttctggat cctgaaagag cagacgattc gtaccaatat tggtcatgtg
    15481 cagatttaca atcagcacta ttttgaaacg tcgaataaaa ataagagcct gattgccgat
    15541 tatgcggcgt taaagaaagc tattttgtcg gacccgacgc tggcggcgga tatttccacg
    15601 ctctccgggc aactggagtt tactggcgta atttcacatt acgagagcga aacttccagc
    15661 tatttctccg cgctgggggt ggagccgtta cccgctttaa agctgggttc gttcgataag
    15721 attatcgcgg ggagcgatct ttctcgggta aaaggtgatg aaatcaccct gggcagcggt
    15781 ctggcgaaaa cgttgaatgc gaaatacgac gactggctgg atgtgatggt ggtcaacacg
    15841 gcgggcgggc agggcgcgtt gtcgctaaaa ctgcgtggca tttttgagtc aggcattaaa
    15901 gattatgacg atgtcgcgat gaagatcccg ctggacaccg cgcagcgcat gatgggtacg
    15961 gatggcgtca gcaaagtgct aattctgctt aaagaagatg acaccgcggc gtttagcaca
    16021 aagctgcgtc agtttatcga acgcaatcat ttgccgctgg tggtcaaaga ctggcgtgat
    16081 gtttcactct tttatcaaca ggttgaaggg ttgctatcgg ggatctattt cttcatcaag
    16141 ctgattgttg cgctgattgt catcttcatg atcggcaatt cgatggctat gaacattgtt
    16201 gagcgcaccc gtgaaataac cactctgcgt gccataggcc tgaagccgct tcacgtcacg
    16261 cgcttgtttc tgacagaagg catttttatt ggtgtgattg gcgccgtcgg cagcctgctg
    16321 gtggggggcg tgttagcctg gattattaat ctttatggta ttgcgatgcc gccatcgcct
    16381 ggacaaacca tcggctatac cgcctttata aaaaccagta acccggaatt gatttgggtg
    16441 acggtggtat tacccatttt gacagcaaca ggcgcctctg tgttaccggc tttacgtgct
    16501 tcccggctga atatttctga cgcatttaaa ttctcatagg gctaattatg aatatggctt
    16561 tttacggcaa gtggtttgct tgcctgtggc tcgctacgtc gtgcgttcag gcggcgagca
    16621 cagataataa ggcgctggag ataattcgtc gcgctgacga aattcgttcg ccgaataaac
    16681 cgtttcgtta cacgctgacg gtaacggaat ataaagcggg cgcaacccag ccagagaata
    16741 aacaggtcct cgatatttcc atgcgcttta tgaaaccgca gggtaatgaa aaagcggatg
    16801 cacgctcgct ggtgcgtttt atttatccgc cgcgagataa aggaaaaatc atgctttccg
    16861 actggtacga tttatggttt tacacgccgg aactgcgacg tcctatgcct atttcacggc
    16921 aacaaagatt aattgggcaa atctctaacg gcgatgtcat tgtcactaac tttgaatatg
    16981 cctatgactc gaccctggtg ggggaagtca cgtgtgctga aaagcagtgt tacaagctgg
    17041 cgctggtgcg taaatcggcg gatatcacct ggccgaaggt tatctattac gttgaaaaag
    17101 acggtgataa tcgcccgtgg aaggcggcct attattcgca ggatgaccag ttaataaaag
    17161 aagtgttgta tcaggatttc cagccggtgt tggggaaaac gcgacccatg aaaataaccg
    17221 ttaccgatgt tcgtcacggc aataactatt cagtgatgga gtacagcgat gttcgtctgg
    17281 aatcgctgcc ggagtttcat ttcaccaaag agtatattca gcgaggtgcg aaatgaaacg
    17341 tacgctgaag atctcttcgt tgctgtgtgt tgcgctgccg ttgacggtac aggcggattg
    17401 cctgtcaggt gacgaggtgg cacaaaacag cgatgtgacg ctttacgagc aggtgtctta
    17461 tgtcaataaa caggcttcgg cctggcagat tgcaggcaaa aatccctaca ctcgtcacaa
    17521 cggttatcag gaggcgggga ttggcatcaa cagtggttgt tcgataatcg acaatacgct
    17581 ggaccttaag ctcaatctgt atggaataaa cgaatatgcg cttaaacccg cagggaaatt
    17641 cgaaacggat gatagccgca cacgtgcgtt aattaaccgc ctcagtttgg tctatagcgc
    17701 ctcggacagc gttcaatttg aagcaggcaa atttgccgcg ccgtcaggaa tgttcttttt
    17761 gcgttcacct tctgatttgc aaacgcatta ttacaccgga ttccagtcga cacgtttgca
    17821 cgatcccaaa atgacatcgg cgtatcaggc gtcatcgtgg ggcgcaaaaa tgaacttgga
    17881 tacacgcgat tatgcgtttt cggcgagcgt cataccgaag ttagccacca tcgacaaacg
    17941 ctatgtgacg tctggcaact ggtctgctaa tcagcaggga aacagcgacg aagcctatct
    18001 gctgagctac agcgatcatc gctttggtga gcacacaccg acagtcaatg tgcggctcgg
    18061 accatcgccg tcactcgcgc tgtctgacag ttatcactac accccgcagc tgacgctgaa
    18121 tgtcgacgca gcctaccacc gctcccaaca atggcggcat ctttcccacc atgagacggc
    18181 gcaggtcgag gaatatcagt ttccggattc gctctatgaa acgaaagatg aaagcggcgt
    18241 ggagctggcg ctgggtgggc agtacaccag tgataatttc agcgtgtttg gcgttgagta
    18301 ttactttcag agcgagggtt attcgcacgc tgaacagcgt cagcagcgcg agttaattga
    18361 cttcctcaat accacaaccg gctatgcgcc actggatcag gcatttgatt cttacaaata
    18421 cctgatggct tcagaaatca gcaataccgc caatcagggg atgctgcagg gtaagcacta
    18481 tctgaacgcc tgggccagtc tgccgctggc gggagagtcg acgctgcagc cttcgctggt
    18541 ggtcaatctc attgatggca gcacgttgct gggcctgcat tattccacgc cgctcagcgc
    18601 tatcagtaac cagcttgaag cctatgccgg tggctatagc gcgctgggaa gtcggtattc
    18661 tgaattttcg ctttttggcg acacgttagg cctttatttg ggctttaaat actatctata
    18721 aggataaaga gatgatgaat aatgcttcac aaccgacagt actgcttttt gccggtcagg
    18781 gtaatccggt tattggcatg ggcagcgatc tttgggatct caacgccagt acaaaacata
    18841 tctgggattg tgccagcgat atttccgggc tgaacctgcg tcgcctgtgt cagaaggggc
    18901 cgatgaatcg ccttattcaa acgacggtac aacaactggc ggtgaccgca atcaatgtaa
    18961 cgctctatac cctgtgccgt gaacgtcttg agaatttaac ggttgtgggg tcctgcggac
    19021 acagcgttgg tgaatacagt gcgctctacg ctgccggagc tatttctctt gagacgctat
    19081 ttggcatgat tcacttcaga gccagcctga tgcatgacga aagccagaga aataaaggcg
    19141 caatgctggc ggttaaaggc atcaaccgcg aacaactgca gacgatgatt acggaatcag
    19201 gcattgcgct ggatattagc tgtgataaca cgccgcgcca gcaggttatt gggggaacgc
    19261 aggctgcgtt gaatgaattt gccacgctgc tgatggccgc tggatacgaa ccggttaaac
    19321 tcggtgtgag cggtgcctgg cacacgcgtt tgatggaaga cggcgttcag gcaatgcgcg
    19381 attatcttgc cggactggat atcgcttcgc cagagcatca ggttctgatg aacgtaacag
    19441 caaaaagcga ggtcgctccg tcaattatca aagaaaatct ctcattacac ttaactcata
    19501 cggtgaaatg gactgaatct ctcgatacat ttctgaatat gccaacctcg gtagccttcc
    19561 ttgaaataag taataagccc tatttgggga atatgttaaa cgattttgct ggtgtggatc
    19621 agcaacgagt tatgcattgt cgtaaagcat ttactgatgc gaaggtattc aaatgattcc
    19681 tgattattta acttttattc gctttcagga taaacgaagt ctgatataca tttatgctat
    19741 tggacttatt ctgataggct tttattggaa aaatgcagga tttacttttc catcagagga
    19801 tattggtgtc gttagtggga ttctggccct ggtgctgtat aattttattt ttgatctcaa
    19861 ggcgtactgg gcttataaat gcgtcacgaa gaatatcgat ttttcgtggt ttaagaaaaa
    19921 gcagaaccac aaaatagaat tatttcttac gcaacctctg gtggcaggat ttctgtcgtt
    19981 aatcatgttg agtgcaatga gttgggggct ataccagcgt ctaccctcgt tatatgcgct
    20041 gttcctgatt tcgttacttg ggccgttggt tatctttctg ttgtttcgga tgatccgcac
    20101 cagttatgtc aagcaggtcg ctatttcagt ggcgaaaaaa gtaaaatata aaagtctgac
    20161 tcgctatgtg ctgctttcgg tgtgcatctc aacggttgtt aacttgctta ctatcagccc
    20221 gttgcgtaac agtgattctt ttgtgataga ggggcagtgg ttaacgttta aatcgataat
    20281 tgcattgctc attctttgtg gcgtagtgtt ggcgattaat ctgttttttc tgcgcttctc
    20341 caggcggtac gcttttttgg gcaggctttt tttgcaggaa atcgatctct ttttctccag
    20401 tgaaaatgcg ttgtcgacct tttttgccaa gccgctttgg cttcggttat tcatattgct
    20461 ggttattgaa gtgatgtgga ttacgctggt gtcggtattg gcaacgcttg tggaatggcg
    20521 gatttggttt gaagcctatt ttttactctg ctatgtaccg tgcttaattt actatttttt
    20581 ctattgtcga ttcctctggc ataacgattt tatgatggca tgtgacatgt atttccgttg
    20641 ggggcatttt aataagtgag gaagtgatag gaagtgacca gataatacat atatgttctg
    20701 tactctcttg cgcattttga ttgttgactg agcaaccaga cagttgatgt gcacgatttc
    20761 ccctcgccct aacagacgtg ggcgggggca cccggtgtgt gcaatatacg aaaaaaaagc
    20821 ccgtactttc gtacgagctc ttctttaaat atggcggtga gggggggatt cgaacccccg
    20881 atacgttgcc gtatacacac tttccaggcg tgctccttca gccactcgga cacctcacca
    20941 aattgtcgtt cctgtcttgc tggaacgggc gctaatttag ggaaatcatg acctgaggtc
    21001 aacaaacttt tttaaaaaat cgcgcgttta ttcaaacttc aatcaatatg tggttttaat
    21061 aagcgaaatc tgcttttttt gccaccgacc acggatttgt tatgctggtg gtctttgtag
    21121 atcataacga taagtgcgaa taaatttcgc acaacgcttt tcgggagtca gtatggatat
    21181 catcttttat cacccaacgt tcgataccca atggtggatt gaggcactgc gcaaagctat
    21241 tcctcaggca agagtcagag catggaaaag cggagataat gactctgctg attatgcttt
    21301 agtctggcat cctcctgttg aaatgctggc agggcgcgat cttaaagcgg tgttcgcact
    21361 cggggccggt gttgattcta ttttgagcaa gctacaggca caccctgaaa tgctgaaccc
    21421 ttctgttcca ctttttcgcc tggaagatac cggtatgggc gagcaaatgc aggaatatgc
    21481 tgtcagtcag gtgctgcatt ggtttcgacg ttttgacgat tatcgcatcc agcaaaatag
    21541 ttcgcattgg caaccgctgc ctgaatatca tcgggaagat tttaccatcg gcattttggg
    21601 cgcaggcgta ctgggcagta aagttgctca gagtctgcaa acctggcgct ttccgctgcg
    21661 ttgctggagt cgaacccgta aatcgtggcc tggcgtgcaa agctttgccg gacgggaaga
    21721 actgtctgca tttctgagcc aatgtcgggt attgattaat ttgttaccga atacccctga
    21781 aaccgtcggc attattaatc aacaattact cgaaaaatta ccggatggcg cgtatctcct
    21841 caacctggcg cgtggtgttc atgttgtgga agatgacctg ctcgcggcgc tggatagcgg
    21901 gaaagttaaa ggcgcaatgc tggatgtttt taatcgtgaa cccttaccgc ctgaaagtcc
    21961 gctctggcaa catccacgcg tgacgataac accacatgtc gccgcgatta cccgtcccgc
    22021 tgaagctgtg gagtacattt ctcgcaccat tgcccagctc gaaaaagggg agagggtctg
    22081 cgggcaagtc gaccgcgcac gcggctacta ataaagcatc aggattcctg ctatccttgg
    22141 cgggaattga atacaggaga gagttatgta tcccgtcgac cttcatatgc ataccgttgc
    22201 cagcacgcat gcatatagca cattgagtga ttatattgcc caggccaaac aaaagggcat
    22261 taaacttttt gcgatcaccg atcatggccc ggatatggaa gatgcgccac atcactggca
    22321 cttcattaac atgcgtatct ggccgcgagt ggttgatggg gtagggatcc tgcgcggcat
    22381 cgaagctaac attaaaaatg ttgatggtga aattgactgc agcggcaaaa tgtttgattc
    22441 gctggatctg attattgccg gttttcatga gccggttttt gcgccacatg ataaagcgac
    22501 caatacacaa gcgatgatcg ccactatcgc cagcggcaat gtgcatataa taagccatcc
    22561 cggaaatccg aaatatgaga tagatgttaa agccgttgct gaagcagccg cgaaacatca
    22621 ggtggcgctg gaaatcaata attcctcatt tttacactca cgtaagggca gtgaagacaa
    22681 ctgtcgtgcg ctagccgcag cggtacgtga tgctggaggt tgggtggcat taggctcgga
    22741 ttctcacacc gcgtttacca tgggggaatt tgaagagtgt cttaaaatcc tcgacgcggt
    22801 agattttccg ccagagcgca ttttgaatgt ttctccgcgc cgcttactga acttccttga
    22861 atctcgcggt atggcaccga ttgcggaatt tgcagacctt taattactag taatggaaat
    22921 atattaatga acgagttttc tatcctctgt cgtgtgctgg gttcgctcta ttaccgccaa
    22981 ccgcaagatc ctttactggt gccgctgttt accctgattc gtgaggggaa actggctgcg
    23041 aactggccac tggagcagga tgagttgctg acacgtttgc agaaaagctg tgatatgacg
    23101 caagtctctg ccgattacaa tgcgttgttt atcggcgatg aatgtgccgt gccgccatat
    23161 cgtagcgcat gggttgagga cgcaacggaa gcggaagtgc gcgcttttct ttctgaacga
    23221 gggatgccat tagcggatac gcctgccgat cacatcggca cattgctgct tgcggcttcc
    23281 tggctggaag atcagtcaac ggaagatgag agcgaagcac tggaaacact gttcagtgag
    23341 tatctgttac cctggtgtgg tgcgttcctt ggcaaagtgg aggcccatgc aaccacgcct
    23401 ttctggcgca ccatggcacc gctaacccgc gatgccatta gtgcaatgtg ggacgagctg
    23461 gaagaagatt ctgaagagta aatgtgatct gcgtcactca taaatgcaac agataatttg
    23521 ctgttgcata aaatgtgtgc tcgatctcat tcatggccgc gttttctgct atcatgcgcg
    23581 gcatgaacat acttctctct attgcaatca caacaggcat tctctccggt atctggggat
    23641 gggtggctgt ttccctgggc ttactaagct gggcgggctt cctgggctgt acggcctact
    23701 ttgcctgccc gcaaggtggg ctgaaagggc tggcgatctc cgctgcaacc ctgcttagtg
    23761 gcgtggtgtg ggcgatggtc attatttacg gtagtgcact gacgccacat ttggaaattc
    23821 tcggttatgt cataaccggt attgtcgcgt ttctgatgtg tattcaggcc aaacagctgc
    23881 tgctttcatt tgttccgggg acgtttatag gcgcatgcgc gacgtttgcc gggcagggtg
    23941 actggaaact ggtgttacct tcgctggcgc ttgggctgat atttggttac gcaatgaaaa
    24001 acagcggcct gtggctggcg gcgcgtagtg caaagacggc gcaccgtgag caggaaatca
    24061 aaaataaagc gtgaggggca ctcacgcttt cgcttaagca gtaaaatgcc ggatgataat
    24121 tccggctttt ttatctgtca ggattccggt ggaaccgaca tatggcggta tttcaccaga
    24181 atgtcattct gccgttctgc tttgttctgc aaatcccaca gaccacggtc aataccatca
    24241 ttaatcagga aaatgacccc tgtttcgata gccgacatca ggcacagcat aacgggttcg
    24301 ttcgaggtgt aacccacttc cccttccagc aagcgctggt agtcaataaa gcggaaaacc
    24361 ccggcctgaa cttcatagga aagtatcgtc ttactagtgt tcaccgagga gaggatctca
    24421 ccggtactca cattgacgac gcgcaggttc acggcaatct gatcgagctg gtattgcgtg
    24481 tcggcaccga tcccaaaata tcttgcccca accccgccag atttgacgtt gctttcataa
    24541 ccgataatcg aaccttcaac catgatattt gccgccgtta aagattgcag cgggattcgg
    24601 ttattaatgg caaccgtgcc gttttcttgt gccgcacgaa taatcttgcg ctcgttaagc
    24661 aggttttgta agccctggcg ctccagcggt ataaaccagc gagaatcttt caatgccgtg
    24721 accagcattg ccgtggcgct ttgcggaaca gcagtggaga agttacttgc cgggtagggt
    24781 ttaaattgcc cggtttcgtc ctgaatgttg tataccgaaa caaagatctt acccgtcggc
    24841 gctggcagat gggtcaaatc tttgtagctc tgagctcgag gcattaatgt cggtctggcg
    24901 gcttctttag gcggggcggt taagcatccg ctcagtaaca tgacggcaac caaaagaaat
    24961 aagcgctgca tgattatttt ccttatgaag ctggggctta aaaatcggtt gagttatttt
    25021 gtaaacccga aacctggata gtcgaggttt gtccggtttt acgatctgtc acgttcaact
    25081 gcaattgacc atcgcggttg gcaatatcga caatataatc gttggtcacc atgcggcccg
    25141 gtttgccggt attaatattc gacagtagcc cacccaaaat ttgtgactgg atggcctgag
    25201 taaagttatc taacgctgag ggtgtttcaa taccatagtc atcgttatag ctcggatctt
    25261 tataagagtt ttgggcctga gcgctattta ataaaaaagc gccattattt gggttaccac
    25321 caaagtttgg attacggaac tggaaagtca tggttccagc ccaacttaat ggcgaaataa
    25381 gcatgagtag aactactgca tgtttgacac gcattgcagc ctccgaacaa ttttttattt
    25441 agaattcatc atgcgccaaa tcgtccgtac ttaataacgc ctgatttatc tggcgacgat
    25501 ttagtgcttc ttcagtttga atcagtgcaa agacgacagt tttctcgaag tctcttttca
    25561 acggaaataa aaaagtctgg aaaataacgt cctgattgac cgttatagtg atccaacttc
    25621 cccatcgtgc actgggcctt tcattaatcg ttaagttacc cgtatagtca ctttcccatt
    25681 tatcactaaa ggctcggtaa aaatcatggc caatagatga aacagtatgg tcagttagca
    25741 atcccgggac ttctacctca acggcgtgaa gattccctgc ggcgaacaga aattctgccg
    25801 ccacaatcca gcgtaaataa cgtttcatgg ctttatcgcc tgaggttatc gtttgcccag
    25861 gaaaccgctt gtgtccggtt ttttacggct atcttcttga aaagattata aagatgcgtt
    25921 ttaaccgtat tttcgctgat gaacaacgaa cgagcgatct cgttattaga cgcgccgata
    25981 cgcagcttat tcaggatctc tttttcccga tgagtaagga gggctgattc cgtgctgtta
    26041 taacgatagt tacccgaatg cgtaatcagg tagctggcaa gcttttgcgt aaagtagcat
    26101 tcgccgcgca ggacgccttg caacccattg acaacatgtt cttgatcctc catggcataa
    26161 aaaacgccgt tgatatgagg ccagttttca atgtcgcggt acgggtaatc ttcaggcgta
    26221 tttagcaaca atattttgat attgttgttt ttcctgctca aagtatcctg ccaataatgg
    26281 ataagctttt tatccgcttc catcatatcc agaagaataa tagagcctga agatatatcg
    26341 tccagagaac gttgaatatt atgtaatttt cctgtaattg ccagcgattg tttaaggtgc
    26401 tgcaagagag ccgtcgcctg caaagaaggt ttagtgatca acaataatgt atgaccatga
    26461 atactatgga cttcattaaa catgatgaaa ccccgctttt tttattgatc gcacacctga
    26521 cagctgcctc taaaatagaa gcaccagaag tactgacaga tgttgcactg ctgtgtgtag
    26581 taataaatca gccctaaa
//
LOCUS       DAZFKF010000068        26110 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFKF010000068 DAZFKF010000000
VERSION     DAZFKF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.068"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1815)
                     /gene="espL1"
                     /locus_tag="ACHKA1_003303"
     CDS             complement(<1..1815)
                     /gene="espL1"
                     /locus_tag="ACHKA1_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFO5680910.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDTPTPSSEPINHFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFRQLPILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLMQTKSANETPG
                     LYISMLYGFDEIIDIFLNTLTAPIAQKHLNKKMVMDILAMKTRDGEPGLFAAMENNHP
                     LCVTRFLSKIYGIAVKYNLSKINIIDLLKGATVHGTPALYIAMSKGNKDVMLSYISTL
                     DTFAKKYSFSQHQLFTLLAAKNHDNMSAVHIAIHHNHYK"
     gene            2338..4266
                     /gene="thrS"
                     /locus_tag="ACHKA1_003304"
     CDS             2338..4266
                     /gene="thrS"
                     /locus_tag="ACHKA1_003304"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO5680911.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4270..4812
                     /gene="infC"
                     /locus_tag="ACHKA1_003305"
     CDS             4270..4812
                     /gene="infC"
                     /locus_tag="ACHKA1_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO5680912.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4909..5106
                     /gene="rpmI"
                     /locus_tag="ACHKA1_003306"
     CDS             4909..5106
                     /gene="rpmI"
                     /locus_tag="ACHKA1_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO5680913.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5159..5515
                     /gene="rplT"
                     /locus_tag="ACHKA1_003307"
     CDS             5159..5515
                     /gene="rplT"
                     /locus_tag="ACHKA1_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO5680914.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5632..5756
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5638..5682
                     /gene="pheM"
                     /locus_tag="ACHKA1_003308"
     CDS             5638..5682
                     /gene="pheM"
                     /locus_tag="ACHKA1_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO5680915.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5965..6948
                     /gene="pheS"
                     /locus_tag="ACHKA1_003309"
     CDS             5965..6948
                     /gene="pheS"
                     /locus_tag="ACHKA1_003309"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO5680916.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6963..9350
                     /gene="pheT"
                     /locus_tag="ACHKA1_003310"
     CDS             6963..9350
                     /gene="pheT"
                     /locus_tag="ACHKA1_003310"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO5680917.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITSEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9355..9654
                     /gene="ihfA"
                     /locus_tag="ACHKA1_003311"
     CDS             9355..9654
                     /gene="ihfA"
                     /locus_tag="ACHKA1_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO5680918.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9755..10735
                     /gene="btuC"
                     /locus_tag="ACHKA1_003312"
     CDS             9755..10735
                     /gene="btuC"
                     /locus_tag="ACHKA1_003312"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO5680919.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            10798..11349
                     /gene="btuE"
                     /locus_tag="ACHKA1_003313"
     CDS             10798..11349
                     /gene="btuE"
                     /locus_tag="ACHKA1_003313"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO5680920.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKFAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11349..12098
                     /gene="btuD"
                     /locus_tag="ACHKA1_003314"
     CDS             11349..12098
                     /gene="btuD"
                     /locus_tag="ACHKA1_003314"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO5680921.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEIRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12177..12641
                     /gene="nlpC"
                     /locus_tag="ACHKA1_003315"
     CDS             12177..12641
                     /gene="nlpC"
                     /locus_tag="ACHKA1_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO5680922.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            12888..13601
                     /gene="ydiV"
                     /locus_tag="ACHKA1_003316"
     CDS             12888..13601
                     /gene="ydiV"
                     /locus_tag="ACHKA1_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO5680923.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVARLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            13664..15100
                     /gene="selO"
                     /locus_tag="ACHKA1_003317"
     CDS             13664..15100
                     /gene="selO"
                     /locus_tag="ACHKA1_003317"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO5680924.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSNSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(15104..15295)
                     /gene="hemP"
                     /locus_tag="ACHKA1_003318"
     CDS             complement(15104..15295)
                     /gene="hemP"
                     /locus_tag="ACHKA1_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO5680925.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(15427..16473)
                     /gene="aroH"
                     /locus_tag="ACHKA1_003319"
     CDS             complement(15427..16473)
                     /gene="aroH"
                     /locus_tag="ACHKA1_003319"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO5680926.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(16630..17463)
                     /gene="ppsR"
                     /locus_tag="ACHKA1_003320"
     CDS             complement(16630..17463)
                     /gene="ppsR"
                     /locus_tag="ACHKA1_003320"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO5680927.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            17796..20174
                     /gene="ppsA"
                     /locus_tag="ACHKA1_003321"
     CDS             17796..20174
                     /gene="ppsA"
                     /locus_tag="ACHKA1_003321"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO5680928.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVEQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(20231..21877)
                     /gene="fadK"
                     /locus_tag="ACHKA1_003322"
     CDS             complement(20231..21877)
                     /gene="fadK"
                     /locus_tag="ACHKA1_003322"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO5680929.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWQEAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(21934..22227)
                     /gene="ydiT"
                     /locus_tag="ACHKA1_003323"
     CDS             complement(21934..22227)
                     /gene="ydiT"
                     /locus_tag="ACHKA1_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO5680930.1"
                     /translation="MSQNATVNVDVKLGVNKFHVDEGHPHIILAANPDIKEFQKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(22224..23513)
                     /gene="ydiS"
                     /locus_tag="ACHKA1_003324"
     CDS             complement(22224..23513)
                     /gene="ydiS"
                     /locus_tag="ACHKA1_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5680931.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIHPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMHHFRKIPALMENPRLFSQYPRMIADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(23569..24507)
                     /gene="ydiR"
                     /locus_tag="ACHKA1_003325"
     CDS             complement(23569..24507)
                     /gene="ydiR"
                     /locus_tag="ACHKA1_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO5680932.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQVQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(24527..25291)
                     /gene="ydiQ"
                     /locus_tag="ACHKA1_003326"
     CDS             complement(24527..25291)
                     /gene="ydiQ"
                     /locus_tag="ACHKA1_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO5680933.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQVGLLAGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DNVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            25607..>26110
                     /locus_tag="ACHKA1_003327"
     CDS             25607..>26110
                     /locus_tag="ACHKA1_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFO5680934.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNA"
BASE COUNT         6513 a         6205 c         6806 g         6586 t
ORIGIN      
        1 tttataatga ttatgatgaa tggctatatg aacagctgac atgttgtcat gatttttagc
       61 ggccaacagt gtaaataact gatgttggct aaaagaatat ttttttgcaa aagtatccag
      121 cgtcgatata taagataaca tgacgtcttt attacccttg ctcatagcga tgtataaagc
      181 aggggttcca tgtactgttg cgccttttaa taaatctatg atgttaattt tgctgaggtt
      241 atatttaaca gcgattccat aaattttaga gaggaaccgt gtgacacaca acgggtgatt
      301 attttccatt gcagcaaata accctggttc accatcacgt gttttcatgg ctaaaatatc
      361 catcaccatt tttttgttta aatgcttttg tgctattgga gcggttaatg tattcagaaa
      421 gatatcgatt atctcatcga atccatacaa catggagata taaagtccag gtgtttcgtt
      481 agcactttta gtttgcatta atttaaccag attatcttca tgaatgatgt gattatctac
      541 cagtgactga atttcttgca taaatatttt cacgttgtca gtatgtccgt tcatcatagc
      601 caaaaataaa gcaggtgtac caacgctatt ctgcgcagtt attatttcaa tgatttcttt
      661 ttcagggagt atgggcaact gtctgaataa tgtaataagc tgttgagtct ggcctgtggc
      721 cattattaac aacattgccg atggatgtat aggggcaaat ggcattcgtg tcgcgatacc
      781 cgtgaaaata gcccctggaa ctccctcagg taataggggc agaatagcaa ttgcacagtt
      841 gcgaacatac ctatcagccc aggtctgagc atcgggacct gaactgatga acttatcaag
      901 cgagagaaag ccccgtgctg tcctgtgggt gcctctgacg gcaacgttgg tatcgttcgg
      961 gtcgtaaacg gacacaacat aatttaattg cccatacgcc gactgtttta ttcgtagcct
     1021 tactgacaaa gtatgattgt ctatatataa aatcgcagac atatgactga cattgtttgc
     1081 catcattcga cagaagaaaa tatctaaagc gaagccaaaa tccttactcg cgataacgcg
     1141 actccctgca ggaggatggc gaagtaatgc tctgaactcc gcgtcccgga ctaaggggac
     1201 atgctgctgt atggattcaa cggaggagta atgtttaatc aggttgacct tgccttttcc
     1261 atttctcaat gaatcaagtg cgtattgcaa tgacagatgg cgacaaaaaa tagcgtcaga
     1321 aggagattgc gaaaaaaatg cactaccatt gagatttact gttggttgta tagttttttc
     1381 tctatggatt tttttagaca gtttcataca atggttacga ttttcggcag ttaactgttc
     1441 ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg tggtttccag
     1501 taataaggaa atgatttctt ttttatagtg taatgtaata agtatatttt ctgcatggat
     1561 gggtgaagtg gcaaagttaa tggtaaaaag ggcagtggaa taacaatttt gtgatatttt
     1621 tcgacagttt gcggtaaatt cctgagtaac acagtatgac agcatcgaac ttaagtcaga
     1681 ggtatcaagc agggtgatga gctggcgcgc aaaatgatta ataggctctg atgaaggcgt
     1741 tggtgtgtcg ctatcgatgt gcattctatg aggagactta taatgagttc tgataataat
     1801 gtcgttttga ctcataatct tttcgcctta tatatgtctg gaggttttga ggatgatgat
     1861 taattattat cgccagaacg aaatgtgcac gaaaaattgc tataagaatt gtgtcacttt
     1921 tatcattgtg atatgaccac ttcgtcatta atctcatgat cgtttaaata ctaccgccaa
     1981 atcatgcaac gaactttgct ggatttagtt tatgctgctg taaatccgct cgcagtaaac
     2041 ctttcagacg ctccgtgatg ttatcagttg ttctttaagc gttttgctgg tgtactcact
     2101 acaaacgaat tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag
     2161 tgttaacatc gctcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg
     2221 attgcgaacc aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca
     2281 aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg
     2341 cctgttataa ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg
     2401 gatgttgcgc tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat
     2461 ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcatcacc
     2521 gccaaagacg aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcac
     2581 gcgattaaac aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt
     2641 ttttattacg acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag
     2701 aagcggatgc atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg
     2761 cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct acaaagtctc cattcttgac
     2821 gaaaacatcg cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg
     2881 tgccgcggcc cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg
     2941 gcaggggctt actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg
     3001 gcgtgggcag acaaaaaagc gcttaacgct tacctgcagc gcctggaaga agccgcgaaa
     3061 cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg
     3121 ggtatggtat tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt
     3181 cgttctaaac tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt
     3241 gtcctgtggg aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacgtct
     3301 tctgagaacc gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttt
     3361 aaccaggggc tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc
     3421 caccgtaacg agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag
     3481 gatgacgcgc atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc
     3541 cgtttagtct atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc
     3601 actcgtcctg aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg
     3661 gcggttgcgc tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc
     3721 tacggtccga aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt
     3781 acagtacagc tggacttctc cttgccgtct cgtctgagcg cttcttatgt aggcgaagac
     3841 aatgaacgta aagtaccggt aatgattcac cgcgcaattc tggggtcgat ggaacgtttc
     3901 atcggtatcc tgaccgaaga gttcgctggt ttcttcccga cctggcttgc gccggttcag
     3961 gttgttatca tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa
     4021 ctatcaaatg cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa
     4081 atccgcgagc acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg
     4141 gaatcaggca aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta
     4201 aatgaagtga tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag
     4261 gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc
     4321 gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg
     4381 agtctgagag aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc
     4441 cctaacgccg agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag
     4501 agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa
     4561 ttccgtcctg gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt
     4621 ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac
     4681 cagcaaatcg gtatggaagt gcttaaccgc gtgaaagacg atttgcaaga actggcagtg
     4741 gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag
     4801 aagaaacagt aaggccttca agtagcactg tctgtggagc cttcgggctt cacagacttt
     4861 gttcgccttt gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt
     4921 aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac
     4981 aagcacgcta acctgcgtca cattctgacc aaaaaagcga ccaaacgtaa acgtcacctg
     5041 cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac
     5101 gcataagccg ttaacgtttt taacttttta attagaatat agatacagga gagcacatat
     5161 ggctcgcgta aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc
     5221 taaaggctac tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa
     5281 agctggtcag tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg
     5341 gattgcgcgt atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa
     5401 tggcctgaaa aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt
     5461 cgacaaagta gcgttcaccg ctctggttga aaaagcgaaa gcagctctgg cataagccag
     5521 ttgaaagagg gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta
     5581 tcgccgtagc cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg
     5641 aatgctgcta ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg
     5701 tgagaagaga aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc
     5761 gcgttttgaa aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggatac
     5821 ggccagtgcg gagaaaccgg agcgtactta agtacgtgag gatttcgagc actgcccggg
     5881 accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc
     5941 accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag
     6001 gcggccatta gccaggcgtc agatgttgcc gcgttagaca atgtgcgcgt cgaatatttg
     6061 ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag
     6121 cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat
     6181 gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc ggaaacgatt
     6241 gatgtctctc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc
     6301 atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa
     6361 atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc
     6421 gctgaccacg acactttctg gtttgacgct acccgcctgc tgcgtaccca gacctctggc
     6481 gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgt
     6541 gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg
     6601 attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga cttcctgcgt
     6661 aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc gtttaccgaa
     6721 ccttctgcag aagtggatgt catgggtaaa aacggtaaat ggctggaagt actgggctgc
     6781 gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt
     6841 ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg
     6901 cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga
     6961 ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgatg
     7021 cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg
     7081 gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg
     7141 acaaactgcg tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgcg
     7201 gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc gaccattggt gctgttctgc
     7261 cgggtgattt caaaattaaa gcggcgaaac ttcgtggcga accgtctgaa gggatgctgt
     7321 gctccttctc tgagctggga atttctgacg atcataacgg cataatcgag ctgcctgcgg
     7381 atgcgccgat tggcaccgac atccgcgaat acctgaaact cgatgacaac accatcgaaa
     7441 tcagcgtaac gccaaaccgt gccgactgtt taggtatcat tggtgttgcg cgtgacgttg
     7501 ccgtgctgaa tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg
     7561 acgacacgct gccgattaca gtcgaagcgc cggaagcctg cccgcgttat cttggccgtg
     7621 tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc
     7681 gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgtg ctgctcgaac
     7741 tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga
     7801 tggcgaaaga gggtgaaacg ctggtgctgc tcgacggcac tgaagcgaag ctgaatgctg
     7861 acaccctggt tatcgccgac cacaacaagg cgctggcgat gggcggcatt tttggtggcg
     7921 aacactctgg cgtgaatgac gaaacgcaaa acgtcctgct ggaatgcgct ttcttcagcc
     7981 cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt
     8041 atgagcgtgg cgttgatccg gcactgcagt acaaagcgat ggaacgtgcg acccgtctgc
     8101 tgatcgacat ctgcggtggt gaggctggtc cggtaattga tatcaccagc gaagcaacgc
     8161 tgccgaagcg tgcaaccatc actttgcgtc gtagcaaact ggatcgcctg atcggccatc
     8221 atattgcgga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag
     8281 gcaaagacga gtggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag
     8341 atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac
     8401 aggcaagcct gattatgggt actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa
     8461 cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag
     8521 tgcagcagat gatccatcca ggcgttgaag ccttactgct gccaagcccg atctctgttg
     8581 aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc
     8641 agaaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata
     8701 ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc
     8761 gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg
     8821 atcttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag
     8881 cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt gaacgtattg
     8941 gttttgttgg ggttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc
     9001 tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga
     9061 tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg
     9121 cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact
     9181 tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc
     9241 tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca
     9301 aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg
     9361 cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat
     9421 gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa
     9481 caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga
     9541 cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga
     9601 cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat
     9661 ctaactaaaa aggccgcgtt gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa
     9721 atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca
     9781 gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt
     9841 aagcctttgc gccggtgaac aatggatctc gccaggtgac tggttttctc ctcgtggcga
     9901 actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc
     9961 gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg
    10021 actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca
    10081 agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac
    10141 tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg
    10201 cgttgcatta gggattatct gtagcgcact aatgacgtgg gctatctact tttccacctc
    10261 tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg
    10321 gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag
    10381 gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccctgtg
    10441 gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca gtgtagcgct
    10501 ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt
    10561 aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct
    10621 ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac
    10681 cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca
    10741 aaaagacggt ctatgattaa aagctaagat ttttactgac cacacccagg agaaacgatg
    10801 caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag
    10861 aagttcgccg gtaatgtgct gttgattgtc aatgtcgcct caaagtgtgg cttaacgccg
    10921 caatatgagc agttggagaa tattcagaaa gcctgggccg atcgaggttt tgtggtgctg
    10981 ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact
    11041 tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaacggc
    11101 gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg
    11161 gaagagagcg gattctatgc ccgtatggtc agcaaaggtc gtgcaccgct gtaccctgat
    11221 gatattttat ggaattttga gaaattttta gttggcaggg acggattggt catccagcgt
    11281 ttttccccgg atatgacgcc ggaagatccc attgtgatgg aaagcattaa actggcgttg
    11341 gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc cgcctggggc
    11401 cgctttctgg cgagattcgg gctggggaga tcctgcacct ggtggggccg aatggcgcgg
    11461 gtaagagtac cttactggcg cgaatggccg gaatgaccag cggtaaggga agcattcagt
    11521 tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctatc
    11581 tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc
    11641 agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg
    11701 acaaactcgg tcgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg
    11761 ctgcggtggt gttgcaaatc acaccacaag ccaatcccgc aggccaattg ctgcttcttg
    11821 atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg
    11881 ctctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacgttgc
    11941 gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag
    12001 aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg
    12061 atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat cttgaaaatc
    12121 gggtaattac gacgctaaat taatacaaga ataaaaaaac agaggaaccg tacggaatgc
    12181 gtttctgcct tattttgatc acagcactgt ttctggccgg gtgtagccac cataaagcac
    12241 cgccgccaaa tgccagactt tctgattcga ttaccgttat tgccggtttg aacgaccagc
    12301 tacaaagctg gcatggcacg ccgtatcgtt atggtggcat gacgcggcgc ggtgtggact
    12361 gttcgggatt tgtggtggtg acgatgcgcg atcgttttga tttgcagctg ccccgagaaa
    12421 ccaaagaaca agcctctatc ggcacgcaaa ttgataaaga cgagttgctg cctggtgacc
    12481 tggtcttttt caaaacgggt tccggacaaa atggtttgca tgtaggtatt tatgatacca
    12541 acaaccaatt tatccacgcc tctaccagca agggagtgat gcgttcctca cttgataatg
    12601 tctattggca gaaaaatttc tggcaggcga gacgaatcta gtatgtcgaa aattggacgg
    12661 cgatgataca tcgccgtcca tttactccag aatacagtag tattttgcag acaaaaattc
    12721 cgtaaatgga ttattcgttt ataatcagga taatctaaaa aaaggacccc tgaaaaagtg
    12781 gttaacatgg aacaaatgaa agttactcct tctgtaattg ttatgtctta tggcctcatc
    12841 ccttttagcc ggatactgaa aaacatcctt cgagagggac ggttaccatg aagatttttt
    12901 tggagaatct ttatcattct gattgctatt tcctgccgat aagagataac cagcaagacc
    12961 ttgtcggcgt tgagttaatt acccatttct ccagtgaaga tggtacggtt cgtatcccga
    13021 cgagtcgggt tatagcgcaa cttaccgagg agcaacattg gcaattattt tcagagcagt
    13081 tggaattact aaaatcatgc caacattttt ttattcaaca taaactcttt gcctggctta
    13141 atttaacgcc acaagtggct agattattat tagagaggga taattacgca ggcgaactat
    13201 taaaatatcc gtttattgaa ctgttgatta atgagaatta cccgcatctc aatgaaggaa
    13261 aagataatcg cggtctttta tccttatcgc aagtgtatcc ccttgtgtta ggtaatttgg
    13321 gagcaggcaa tagcacaatg aaagcggttt ttgatggtct tttcacccgc gtcatgctgg
    13381 ataaaagttt tattcagcaa caaatcacgc accgctcctt cgaaccgttt attcgtgcga
    13441 tccaggcgca aatttcccct tgttgcaatt gcatcatcgc gggaggcatt gatacggcag
    13501 aaatattggc acagattacc cccttcgatt tccatgctct ccagggatgc ctgtggcctg
    13561 ccgttcccat aaatcagata acgacgttgg ttcagcgata accctcctgt ttgctggtgt
    13621 ttaagacgag agtaaccgtc tacactatca aacaggagga tctatgaccc tgtcttttat
    13681 tacccgctgg cgcgatgaat tgccagcaac ctatacagca ctttccccta cgcctttaaa
    13741 taatgcccgg ctgatttggc ataataccga actcgctaac acgctgagta ttccatcgtc
    13801 gctgtttaaa aatggcgcag gtgtctgggg cggcgaaacc ctactgcctg gtatgtcacc
    13861 actggcgcag gtttacagtg gtcatcagtt cggcgtctgg gcgggccaac tgggtgatgg
    13921 gcgcggcatt ttacttggcg aacaactgct tgctgatggc actacaatgg actggcatct
    13981 gaaaggtgct ggcctgacgc cttattcgcg aatgggtgat ggacgggcgg ttttacgttc
    14041 gacgatacga gaaagtctcg ccagtgaggc gatgcattat ctgggcattc cgacgacccg
    14101 cgcgttaagt atcgtcacca gcaattcgcc agtgtatcgg gaaacggtgg agccaggcgc
    14161 gatgctgatg cgtgtggcac caagtcatct gcgctttggc catttcgaac atttttacta
    14221 tcgacgcgag ccggaaaagg ttcgtcagtt ggctgacttt gccattcgtc attactggtc
    14281 acatcttgaa gatgatgagg acaaataccg tctctggttt aacgatgttg tcgcacgtac
    14341 cgcatcgtta attgcccaat ggcagacggt cggctttgct catggggtga tgaataccga
    14401 caacatgtcg ctgctgggac tgacgcttga ttacgggccg tttggttttc ttaatgatta
    14461 cgaacccggt tttatttgta atcactcgga tcatcaaggg cgttacagct ttgacaatca
    14521 acctgctgtc gcgttgtgga atttacagcg tctggcgcag acattgtcac catttgttgc
    14581 cgtagatgcc ctgaatgagg ccctggacag ctatcagcag gttttgttga cgcattatgg
    14641 acaacggatg cggcagaaac tgggcttcat gacggagcaa aaagaggata acgcgctact
    14701 gaatgaatta ttcagtctga tggcgcgaga gcgcagcgat tatacccgca catttcgcat
    14761 gctgagtctg accgagcagc acagcgcggc gtcaccgcta cgtgatgagt ttattgatcg
    14821 tgcggcattt gatgactggt ttgcccgtta tcgagggcgt ttgcaacaag acgaggttag
    14881 cgatagtgag cgtcagcagc tgatgcaaag cgttaacccc gctctggtat tgcgcaactg
    14941 gttggcgcaa cgggcgattg aagcggcaga aaagggtgat atgacggaat tgcaccgcct
    15001 gcatgaggcg ttgcgaaatc ctttcagcga cagagatgat gactatgtca gtcgtccacc
    15061 tgactggggt aaacggctgg aagtcagctg ctcgagttaa aggctacttg gtcaacagca
    15121 gcttgccagc ctgagtttta cggagcagat attcttgccc gtcatgatcg ataatcagtt
    15181 tgccatccgg acctaacagt gtctggctgg aaattcgccg aataggctgc ggggaagcgg
    15241 tcttgtgatt ggcatccgtt tcaggcgtga gttttctgct atccgtataa cgcataaaaa
    15301 ttaataataa ttgatataac aatgattctt attatcaata ttatggctga ccagagcaag
    15361 cagtaatgtg ttgagaagac aacaagttgc gcagaaatgc ccatgccgtc aggaatgggc
    15421 acgcattcag aagcgggtat ctaccgcaga ggcgagtttt tcgaccaggc gttcggtatc
    15481 ttcccagccc agacacgggt cggtaatgga ttgaccgtaa gtgagcggct gactgccgac
    15541 gattttttgc gttccttcgc gcaggaaact ttccgccata attccagcaa tcgccgtaga
    15601 gccattgcgg atttgctgac aaatatcctc acaaacttct aactggcgac ggtgctgctt
    15661 ctggcagtta ccgtggctga aatcgaccac cagatgttca ggtaaatcaa actcgtgcag
    15721 cgtatcgcag gctgcggcga tatcatcggc atgataattc ggttttttgc cgccacgcat
    15781 aataatgtgg ccatacgggt tgccgctggt ctgatagatg gtcatctgac catttttgtc
    15841 tggcgagagg aacatatggc tggcgcgggc tgcgcggata gcatccacag caatccgcgt
    15901 gttgccatcg gtaccatttt taaaacctac cggacaggag agtgccgaag ccatttcacg
    15961 gtggatctga ctttcggtag tacgtgcgcc aatcgcgccc caactgatta aatcagcaat
    16021 aaactgaccg gtcaccatat cgaggaactc agtcgcggta gggacaccca gctcatttac
    16081 ctgtaaaagt aatttgcgcg ccagctccag accgtgattt acccgatagc tgccgtttaa
    16141 atctggatcg gagattagtc ctttccagcc gacaacagtt cgtggttttt caaaataggt
    16201 gcgcattacg atttccagcc gtgactggta ctggttgcgc agcgactgca gacgggtggc
    16261 gtactccatt gcagcggtga gatcgtggat cgagcagggg ccaataatga ccaacagtcg
    16321 cttatcttca ccattgagta ttttttcaat tctgcggcgg gagtcggtga catgggtggc
    16381 gacgccaggc gttacgggat accgtagcgc gagttcggcg ggcgttacca ggctctcaat
    16441 acgcgcagta cggagttcgt cagttctgtt cattacaagt ctcagaaatt tgttgcttct
    16501 acggcaggac tgccgggaag tgatgcgcat cacgataaac caatcgctgc tgatttcaag
    16561 tgcgaggtgt gtcacacctc gccgggtggt tagcatgata acaaaaaaat aagctaatgc
    16621 actagttctc tagtacattc ggcgactaag gcccatgata tcgaggatct tggtggcaat
    16681 ctcttctacc gaataattgg tactgttaat ccacgggatc tgatttttac ggtacaaggc
    16741 ttccacttcc gcgacttcca tcctgcactg acgaagcgag gcatagcgac tgttctcccg
    16801 acgttcctcg cgaatcgccg ccagacgttc cgggtcgata gtcaggccga acaatttatg
    16861 ctgaagcggt ttgagcgacg cgggtagcac cagattatcc atatcgtcgg caataaaggg
    16921 gtagtttgcc gcgcggatac caaattgcat tgccagatac agactggtgg gggttttacc
    16981 acagcgagaa acaccgagca ggatcacctg agcctgatcc agattgcgca acgaaatgcc
    17041 gtcatcgtgg gcgagggtgt aatcaatcgc cgcaatgcgc gcatcatatt tattgagatt
    17101 attagggtta aggccatggg tacgatgagc aatcggcgtt ggatccagtt tcatctcttg
    17161 ttgtagcggg gcaaccagcg cctgaacgat atcctggcaa aagccttcac tttgcaagat
    17221 gatggcgcga atctccggca acacgatgga gtagaagacc agcgggcgca cgcctgtctg
    17281 gtgataaatt gcgtcaatct gatccttcac tgcccgtgca cggctctcat tttcgacaaa
    17341 cggcagcgtg atgctgctga tagtgacggg aaattgtgac attactgcgt gtcctaatac
    17401 ctccgcagtt attgccgtac catcagaaat ataaaaaacg tggcgatcaa cagcattatc
    17461 cattttgttc ttcccgtgat gcagacataa ttctatgaaa gcataaatta aaaacgcaca
    17521 gaagcgtaga acgttatgtc tggtttataa aatgaacctt caattttatt ttttatgaaa
    17581 atagcatttc atttttatgg tttcgtttat accgatggtt tatgtggaaa ttgtcgaaga
    17641 gagcagattt gcgcaacgct gggatcagtc ttaaaaagta aaaaaatata tttgcttgaa
    17701 cgattcaccg tttttttcat ccggttaaat atgcaaagat aaatgcgcag aaatgtgttt
    17761 ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc caacaatggc tcgtcaccgc
    17821 tggtgctttg gtataaccaa ctcggcatga atgatgtaga cagggttggg ggcaaaaatg
    17881 cctccctggg tgaaatgatt actaaccttt ccggaatggg tgtttccgtt ccgaatggtt
    17941 tcgccacaac cgccgacgcg tttaaccagt ttctggacca aagcggcgta aaccagcgca
    18001 tttatgaact gctggataaa acggatattg acgatgttac tcagcttgcg aaagcgggcg
    18061 cgcaaatccg ccagtggatt atcgacactc ccttccagcc tgagctggaa aacgccatcc
    18121 gcgaagccta tgcacagctt tccgccgatg acgaaaacgc ctcttttgcg gtgcgctcct
    18181 ccgccaccgc agaagatatg ccggacgctt cttttgccgg tcagcaggaa accttcctca
    18241 acgttcaggg ttttgacgcc gttctcgtgg cagtgaaaca tgtatttgct tctctgttta
    18301 acgatcgcgc catctcttat cgtgtgcacc agggttacga tcaccgtggt gtggcgctct
    18361 ccgccggtgt tcaacggatg gtgcgctctg acctcgcatc atctggcgtg atgttctcca
    18421 ttgataccga atccggcttt gaccaggtgg tgtttatcac ttccgcatgg ggccttggtg
    18481 agatggtcgt gcagggtgcg gttaacccgg atgagtttta cgtgcataaa ccgacactgg
    18541 cggcgaatcg cccggctatc gtgcgccgca ccatggggtc gaaaaaaatc cgcatggttt
    18601 acgcgccgac ccaggagcac ggcaagcagg ttaaaatcga agacgtaccg caggaacagc
    18661 gtgacatctt ctcgctgacc aacgaagaag tgcaggaact ggcaaaacag gccgtacaaa
    18721 ttgagaaaca ctacggtcgc ccgatggata ttgagtgggc gaaagatggc cacaccggta
    18781 aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc acgcggtcag gtcatggagc
    18841 gttatacgct gcattcacag ggtaagatta ttgccgaagg ccgtgctatc ggtcatcgca
    18901 tcggtgcggg tccggtgaaa gtcatccatg atatcagcga aatgaaccgc atcgaacctg
    18961 gtgacgtgct ggtcactgac atgaccgacc cggactggga accgatcatg aagaaagcat
    19021 ctgccatcgt caccaaccgt ggcggtcgta cctgtcacgc ggcgatcatc gctcgtgaac
    19081 tgggcattcc ggcggtagtg ggctgtggtg atgcaacaga acggatgaaa gacggtgaga
    19141 acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt ctatgcggag ttgctggaat
    19201 ttagcgtgaa aagctccagc gtagaaacga tgccggatct gccgttgaaa gtgatgatga
    19261 acgtcggtaa cccggaccga gctttcgact tcgcctgtct gccgaacgaa ggcgtgggac
    19321 ttgcgcgtct ggaatttatc atcaaccgta tgattggcgt ccacccacgc gcactgcttg
    19381 agtttgacga tcaggaaccg cagttgcaaa acgaaatccg cgagatgatg aaaggttttg
    19441 attctccgcg tgaattttac gttggtcgtc tgactgaagg gatcgcgacg ctgggtgccg
    19501 cgttttatcc gaagcgcgtc attgtccgtc tctctgattt taaatcgaac gaatatgcca
    19561 acctggtcgg tggtgagcgt tatgagccag atgaagagaa cccgatgctc ggcttccgtg
    19621 gcgcgggacg ctatgtttcc gacagcttcc gcgactgttt cgcgctggag tgtgaagcag
    19681 tgaaacgtgt gcgcaacgac atggggctga ccaacgttga gatcatgatc ccgttcgtgc
    19741 gtaccgtaga gcaggcgaaa gcggtggttg aggaactggc gcgtcagggg ctgaaacgtg
    19801 gtgagaacgg gctgaaaatc atcatgatgt gtgaaatccc gtccaacgcc ttgctggcag
    19861 agcagttcct cgaatatttc gacggcttct caattggctc aaacgacatg acgcagctgg
    19921 cgctcggtct ggaccgtgac tccggcgtgg tgtctgaact gttcgatgag cgcaacgatg
    19981 cggtgaaagc actgctgtcg atggcgattc gtgccgcgaa gaaacagggc aaatatgtcg
    20041 ggatttgcgg tcagggtccg tccgaccacg aagattttgc cgcatggttg atggaagagg
    20101 ggatcgatag cctgtctctg aatccggaca ccgtggtgca aacctggtta agcctggctg
    20161 aactgaagaa ataaaataaa tacccggcgg cgttttgtcg ccgggtttat gtgatccccg
    20221 aagatgaaac ttattcaatc tcttcacaga catcctgagt taatcgccgc ataatatctt
    20281 tccttaacaa aaacttttgt attttacctg aggcagtgcg cggtagtttt tcgattacca
    20341 cgatatgttc aggatattta tattttgcga cccgtttacg gctaaaaaaa gccaccacct
    20401 cttccagcga taatgaatga tgcggcgctt tcagcacgac ataagcgcat gaacgttcac
    20461 ctaagcgttc atcgggcatt gcaaccacac aagcatcgtg aattttagga tgctgtaata
    20521 aaatatcttc cacttcacgg ctgctaatat tttcgccgcc gcggacaata atatctttct
    20581 tgcgcccggt tatttttata tagccagcct catccatgcg gcagagatcg ccgctgtaat
    20641 accagccttc ttcatccagg gcacgggcgg ttaattcagg ttcatcaaaa taccccataa
    20701 acacattggg gccacgcgag gcttcttcac cttcgcaacc tggcggtaag gtcttgcgtg
    20761 cgtcatcgac cactttaatc tctacacctg cggcagcgta accatcggtg tgcataaagc
    20821 gcgacaaagg atcatcgaga ttcaccaccg catgcggcga actttctgtg gaaccataaa
    20881 cacttaataa tttaatgccg cgctgctggc attcacgcgc cacttttttg gggattgtgg
    20941 taccgccgca aagaaagaag cgcagcgctg aaaggtctgc gggttgtttc tctagtaaat
    21001 tcaaaagatc atagacaaac ggcgttgcgc cgagcataca ggtgcaacgc tgctgctcaa
    21061 gcagcgcgag acacgcatca ggagtgaaaa tatctaacaa cacgctgcga gcgccaatta
    21121 agaatggtgc cgttacgcca tgcagaaagc ccgttgcgtg gccgagtggc gcaggcatca
    21181 taaagacatc ctgccaggtc agattcagcc gcgcgcaata agcccgctca ctggcgagaa
    21241 tattgttatg cgttagcatc acgccctttg gcagaccctc ggttccggag gtaaacagca
    21301 ccgcggctaa ttcatcgccg tggaccgtta tcgccgtggt cagtggggta ttgtcggcga
    21361 taatctgact taatgagagg gaagaggtgg cgggagccag tttgtccacg ccgacaattt
    21421 gttgtagttg tggaagctga ttttgcagcg gcaggattaa atctaccgga cgcgtttgtt
    21481 taaacaacgt cggtgcaaag aacatttttg cctgacactt attgagcacc cacaccagtt
    21541 ctgcttcctg ccaggaaggc aacagcggca cggaaaccgc accgattttc aggcaggcaa
    21601 gatagataac ggtaaattca caccagcctg gcagttgaaa tgcgatgcga tcgcctgatt
    21661 caataccctt cgctaacatc cagtttgcca gacagctcgc ggcgtgatcg agcgcgctat
    21721 aggtgtacga tgctccatga ttatcgacca cggcaatttt gtctggcatc gcacgagcgg
    21781 tctgctgcca gtaatcggcc agcgaagcat cgccccatag cccttgctga cgatacgccg
    21841 cacgacgttg ttcgttaaac gttaatgtca ctttcatttt ggactctcga aacagaacat
    21901 caggcccgag atgcgggcct gtgggatgca tgattagccg tagcgaaaat caataccgaa
    21961 ggtgcctgcg ggatattgcc actgttcgag aatagtgtta ccgcacaaca cccgacaggt
    22021 gccgcactcc agacaaccgg cagaatcaaa atgaatgttg ccggcgtcat cctgcttata
    22081 aagtccggca gggcaggctt tcattaattt ttgaaattct ttgatgtcgg gatttgccgc
    22141 cagaataata tgtggatggc cctcatcaac atggaattta ttgacgccta atttgacatc
    22201 aacgttaacc gtagcgttct ggctcatagc gcggttgctc ccttaatgcc atctttcagc
    22261 agattgatca gcccgatttt cttcgcgtgt cccatgatca ttttgcgtac cggctggttt
    22321 ggtttgccgt caatggtgaa catatcgttc atgatgtcgg cgatcatgcg cggatactga
    22381 ctaaacaggc gcggattttc catcagcgcc gggatcttgc gaaaatggtg catatcgcgc
    22441 ataacacaac tttgttccag ctcacgtttg tattgcgcaa gactgctggc ggagaaatcc
    22501 gcgcgttctt tggcggcgat caccgttgtg gcggcagcct gagccgatgc aatggctaaa
    22561 tccatgccgc ggaccgtaaa acccaaattc aggcagaagc ctgcggcgtc accaacgatc
    22621 atcacgccct cgttaaccag ttgcggcacc attgccagac cgccttccgg caccatatgc
    22681 gcggaatatt caagcagttt gccgccgcta atcagcgggt gaatggcggg gtgttgttta
    22741 aaatcttcca gcatttgcgg cacgcttttt tgcgcatggg cgatatcacc caatccacaa
    22801 accagcccca acgatatgga atccttattg gtatagagga atccgccacc catcaggccg
    22861 tcagaagggg aaccggcaaa cagccaggcg gcaccttcct cgcccgtaat attaaagcga
    22921 tcgttgatct gttctggtgt gaggccaata acctctttaa caccaacagc gtaatgatgc
    22981 ggatcggaag cgggaaccat tcccagcgaa cggccaagca tcgagttaac gccatcagcc
    23041 agaatcacca cattcgcttc gagaatatca tccccagcct ggacgccagt gaccttgttt
    23101 ccttcacgaa ccagcgcatc gacgcgaact cccgggataa actgtgcgcc cgcctgctcg
    23161 gcttgttcca tcaaccacgg gtccagacga ttacgcaata cggtataaga tgcgtgttgc
    23221 ggaacatctg gttgctcgcg gtgaaaatcg agggtaacgg cgctctcttc ggttaagaag
    23281 gagattttct cgcgtgtgac cttgcgttct accggcgctg aggctgcaaa gcctggaatg
    23341 attgcttcaa gtgtgtgggc ataaagacgc ccgccggtca tgtttttaca tccggcactg
    23401 tcgccgcgtt ctatcaccag gacatccagc ccggctcgtg ccatgaccag tgcggcaacg
    23461 ctaccagcaa cgcccgcacc gaccacaatg gcatcaaatt tgtcatccga catgcgttat
    23521 gctcccttcc cgctgcgttt acagcgggag atgtggttga aaggggaatt agcggctcaa
    23581 ctggctaatc agggcaggga cgactttgta gatatcgccc accagaccgt agtcggcata
    23641 gttgaagatt ggcgcatttt tatctttatt gatggcgaca atcacttttg cgccgttgcc
    23701 gccaaccata tgttggatct gcccggagat ccccagcgtc aggtagagat cggatttcag
    23761 caacacgccg gagacaccga tataacgttc acgctccatc cagttctcgc cttcggcaat
    23821 tggacgtgaa cagccgactt cagcattcag caccgccgcc agttcgtgga ccattttcag
    23881 gtcatcctgc gctgccagac cacgaccgac gccaaccaca cgttttgctt tgctcaggtc
    23941 cacgctgctt gccgctttcg cacggcgctc gcgacagaga atttcatgac gcggagcaac
    24001 ataaggtacc gtttctgtcg ggcactgatg agaggtatca ctggtgcacg gttcctgaac
    24061 accgggtgca agggtaatga tcgctagcga gctgttgatc ttttcctgag cgaacgctaa
    24121 cccgccatac atccggtgtt cggcgcaaat gtgaccatcg acaatatcca ccgccgtggc
    24181 atcgttcacc agcgccgcat tcagttgcac acttaaccgt gctgccagag ctttaccacg
    24241 tttcgtcgcg gccagtagca acatagcggg gtgtttatct ttcagcagag cagcaatgct
    24301 ttcggcgtaa ttttcagtgc gttgcagcgc gtcgttttgc tcaagaacat aaatacattt
    24361 tggaccataa ggcataactg cctgcacctg gtcggtattt tgtacaatgg catacacctg
    24421 ttggccccat tgctgagcgc cgccaaacag ttcagcataa cgttcaggat tatcgctaaa
    24481 gacccagacg ctgtttaatt gactcatcgt ttcttctccg taggcttcag ttcagggctt
    24541 tcttcagatg ttccgccagc tcggcaatgg cctccggcga atcgttatcg agaatgatgt
    24601 gcttacgttc tgtttgcggc ggtacgcgaa tgccggtcag ttcagcaagt ggtgcgctct
    24661 ggctccagcc aatatcactt gcctgccact gatttaccgg ttttttaccc gcgccgagaa
    24721 tggctttcat cgaaggaatg cgtggcacgt tgatatcgga ggtgacgcag agcacggctg
    24781 gaacagagag ttcaataact tcaacgttat cttcaagcgt gcgttcaatc accagtgtat
    24841 tgccctgacg ctgaatagca ctcactgcat taatcaccgg aagttgcaga atttctccgg
    24901 ccagcaagcc aacctgctgg gcataaaggt cgccggaacc ttcaccaaag atcagtaaat
    24961 cgaagccgat cttttcaact gctgccgcca gcgcttttgc ggtatcgaga ggcagtgcat
    25021 gttcaagttg cgcatcctgc accaaataca ggctgtgcgg cccgcgggat aacacgtctt
    25081 tgcgcacttt cgagttctgc aacaatgagc cgccaacggt cagcgcggct atctcatcgt
    25141 catctgttgc aagctggctt gcagcttcaa tggcattgag atcgaactgg ctgattttgg
    25201 cgtcggcatt gtcgaaattc agggtgtatt ctggagtgac aacaatgtcc tgttcttcag
    25261 gcaccagctt aaagcaggtt attattttca tgatttctcc tgtgagtcgc atacggaacc
    25321 atctccgatg gaaggtaaaa atggcctgaa aatagtgtgc gatagcggtg cggggttttc
    25381 gacggagcaa gctgttttcc ttgccaacaa tcttgcaaaa aaaatgcgat cgctaaaaca
    25441 aaagtgcaat ttgcggcaat atagttattc catgaccgta caatcttgca ttcagtgtaa
    25501 ataatcttgt ttagctctcg ctaaacgcgg ttgtataacc gcaatacacg ccgtatttta
    25561 tccggcatat attgtttttg cttgctcatg gaatagggag gttgtcatgt atcaacgctg
    25621 ttttgataat gccagtgaaa cgctgtttgt cgccggtaaa acgccacggc tttcacgttt
    25681 tgcatttagc gatgatccaa aatgggagtc tggacatcac gttcatgaca atgaaaccga
    25741 gctgatttac gtcaagaaag gggtggcaag gtttaccatc gattcttcgt tatatgtcgc
    25801 gcatgcagat gatatagtgg tgatagaacg cggcaggctg catgcggtgg cctctgacgt
    25861 taacgatccg gcaacgacgt gtacctgtgc gctgtacggc tttcagtttc agggggctga
    25921 ggaaaatcat ctactgcaac cgcattcttg tccggtaatt gccgcggggc agggaaaaga
    25981 agtcattaaa accttattta atgagctaag tgtgattttg ccgcaaagta aaaatagcca
    26041 aacatcttcg ttatgggatg catttgccta tacattagca attctttact acgaaaactt
    26101 taaaaatgct
//
LOCUS       DAZFKF010000069        26057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFKF010000069 DAZFKF010000000
VERSION     DAZFKF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.069"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            198..392
                     /gene="bfd"
                     /locus_tag="ACHKA1_003328"
     CDS             198..392
                     /gene="bfd"
                     /locus_tag="ACHKA1_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO5680935.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            465..941
                     /gene="bfr"
                     /locus_tag="ACHKA1_003329"
     CDS             465..941
                     /gene="bfr"
                     /locus_tag="ACHKA1_003329"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO5680936.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(938..1405)
                     /locus_tag="ACHKA1_003330"
     CDS             complement(938..1405)
                     /locus_tag="ACHKA1_003330"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO5680937.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHKA1_003331"
     CDS             1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHKA1_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO5680938.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2128..2757
                     /gene="rplC"
                     /locus_tag="ACHKA1_003332"
     CDS             2128..2757
                     /gene="rplC"
                     /locus_tag="ACHKA1_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO5680939.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2768..3373
                     /gene="rplD"
                     /locus_tag="ACHKA1_003333"
     CDS             2768..3373
                     /gene="rplD"
                     /locus_tag="ACHKA1_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO5680940.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3370..3672
                     /gene="rplW"
                     /locus_tag="ACHKA1_003334"
     CDS             3370..3672
                     /gene="rplW"
                     /locus_tag="ACHKA1_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO5680941.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3690..4511
                     /gene="rplB"
                     /locus_tag="ACHKA1_003335"
     CDS             3690..4511
                     /gene="rplB"
                     /locus_tag="ACHKA1_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO5680942.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHKA1_003336"
     CDS             4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHKA1_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO5680943.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4821..5153
                     /gene="rplV"
                     /locus_tag="ACHKA1_003337"
     CDS             4821..5153
                     /gene="rplV"
                     /locus_tag="ACHKA1_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO5680944.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHKA1_003338"
     CDS             5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHKA1_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO5680945.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5885..6295
                     /gene="rplP"
                     /locus_tag="ACHKA1_003339"
     CDS             5885..6295
                     /gene="rplP"
                     /locus_tag="ACHKA1_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO5680946.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHKA1_003340"
     CDS             6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHKA1_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO5680947.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHKA1_003341"
     CDS             6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHKA1_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO5680948.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6905..7276
                     /gene="rplN"
                     /locus_tag="ACHKA1_003342"
     CDS             6905..7276
                     /gene="rplN"
                     /locus_tag="ACHKA1_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO5680949.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7287..7601
                     /gene="rplX"
                     /locus_tag="ACHKA1_003343"
     CDS             7287..7601
                     /gene="rplX"
                     /locus_tag="ACHKA1_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO5680950.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7616..8155
                     /gene="rplE"
                     /locus_tag="ACHKA1_003344"
     CDS             7616..8155
                     /gene="rplE"
                     /locus_tag="ACHKA1_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO5680951.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHKA1_003345"
     CDS             8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHKA1_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO5680952.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHKA1_003346"
     CDS             8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHKA1_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO5680953.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8914..9447
                     /gene="rplF"
                     /locus_tag="ACHKA1_003347"
     CDS             8914..9447
                     /gene="rplF"
                     /locus_tag="ACHKA1_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO5680954.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9457..9810
                     /gene="rplR"
                     /locus_tag="ACHKA1_003348"
     CDS             9457..9810
                     /gene="rplR"
                     /locus_tag="ACHKA1_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO5680955.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHKA1_003349"
     CDS             9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHKA1_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO5680956.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHKA1_003350"
     CDS             10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHKA1_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO5680957.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10515..10949
                     /gene="rplO"
                     /locus_tag="ACHKA1_003351"
     CDS             10515..10949
                     /gene="rplO"
                     /locus_tag="ACHKA1_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO5680958.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10957..12288
                     /gene="secY"
                     /locus_tag="ACHKA1_003352"
     CDS             10957..12288
                     /gene="secY"
                     /locus_tag="ACHKA1_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO5680959.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHKA1_003353"
     CDS             12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHKA1_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO5680960.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHKA1_003354"
     CDS             12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHKA1_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO5680961.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHKA1_003355"
     CDS             12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHKA1_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO5680962.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHKA1_003356"
     CDS             13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHKA1_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO5680963.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHKA1_003357"
     CDS             14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHKA1_003357"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO5680964.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHKA1_003358"
     CDS             15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHKA1_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO5680965.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15545..15913
                     /locus_tag="ACHKA1_003359"
     CDS             15545..15913
                     /locus_tag="ACHKA1_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO5680966.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15924..16349
                     /gene="zntR"
                     /locus_tag="ACHKA1_003360"
     CDS             15924..16349
                     /gene="zntR"
                     /locus_tag="ACHKA1_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO5680967.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16405..16623
                     /gene="arfA"
                     /locus_tag="ACHKA1_003361"
     CDS             16405..16623
                     /gene="arfA"
                     /locus_tag="ACHKA1_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO5680968.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHKA1_003362"
     CDS             complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHKA1_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO5680969.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHKA1_003363"
     CDS             complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHKA1_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO5680970.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHKA1_003364"
     CDS             complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHKA1_003364"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO5680971.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDNWLALLDEAGMKGFPHTDYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHKA1_003365"
     CDS             complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHKA1_003365"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO5680972.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHKA1_003366"
     CDS             complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHKA1_003366"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO5680973.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21494..22618
                     /gene="dprA"
                     /locus_tag="ACHKA1_003367"
     CDS             21494..22618
                     /gene="dprA"
                     /locus_tag="ACHKA1_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO5680974.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWVAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22590..23063
                     /gene="smg"
                     /locus_tag="ACHKA1_003368"
     CDS             22590..23063
                     /gene="smg"
                     /locus_tag="ACHKA1_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO5680975.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23092..23634
                     /locus_tag="ACHKA1_003369"
     CDS             23092..23634
                     /locus_tag="ACHKA1_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO5680976.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPICGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHKA1_003370"
     CDS             23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHKA1_003370"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO5680977.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVAALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24216..25034
                     /gene="aroE"
                     /locus_tag="ACHKA1_003371"
     CDS             24216..25034
                     /gene="aroE"
                     /locus_tag="ACHKA1_003371"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO5680978.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLMRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQTLGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25031..25288
                     /locus_tag="ACHKA1_003372"
     CDS             25031..25288
                     /locus_tag="ACHKA1_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO5680979.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25264..25818)
                     /locus_tag="ACHKA1_003373"
     CDS             complement(25264..25818)
                     /locus_tag="ACHKA1_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO5680980.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6685 a         6164 c         6763 g         6445 t
ORIGIN      
        1 aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc ttcggcgccc
       61 tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc gctgaactat
      121 ggcgcaaata aaactgtgct attgtaatca taactattct catttacact ttgcgcagaa
      181 tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc
      241 gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag tttattcctg
      301 tgggaaatca atgtggtaag tgcattcgcg ccgcgcgcga agtgatgcag gacgagttga
      361 tgcagatgcc agaatttaaa gagattgcct gaagcccacg cttatttttt gacatcccgc
      421 tagccccatc tacgctctaa tagtggaagc ggagggacta taaaatgaaa ggtgatgtta
      481 aaatcataaa ttatctcaat aaactattgg gaaatgagct tgtcgcaatc aatcagtatt
      541 ttctccatgc ccggatgttt aaaaactggg gactgatgcg tcttaatgac gtcgaatacc
      601 atgaatccat cgatgagatg aaacacgctg ataaatatat cgagcgtatt ttgtttttag
      661 aaggaatccc caacttacag gatttaggta agctagggat cggtgaagat gtcgaagaga
      721 tgctgcaatc cgacctgcga ttagagctgg aaggtgcgaa ggatctgcgt gaggctatcg
      781 cgtatgccga cagcgttcat gactatgtta gccgcgatat gatgatcgag atccttgccg
      841 atgaagaagg tcatatcgac tggctcgaaa ctgaactgga tctcatcggt aaaatcggcc
      901 tacaaaatta cctgcaatca caaattaaag ttaaagatta acggcctatc agcaaactat
      961 cccagcctat aacgaaaccc gcagccgcca gaaatggccc aaaaggcagc gggtttttta
     1021 atgattcttt tcctctcatg agcagaccaa taactacggc tccacaagcg aatgaggcag
     1081 caaggaaaac cagtcgtggt aagaaggccc atgaatgcca ggcgccaagg gcggcgagaa
     1141 acttcacatc accatagcct aatccttctt tatggcgcag tatgcgatac ccccagtaaa
     1201 tgacagcgaa tgtgccgtaa ccaatgattg ccccccataa cgcatcggct aaaccatcag
     1261 gatgacaaac ttgatagaac aaaagacctg accagagtaa cgggcacgta aatctgtcag
     1321 gtaacaagcc atgctttgca tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa
     1381 ttagaaaggg aagggtagtg tccataaaac atcctctgta attggagtat caccatcgaa
     1441 taactccgga tgctcagcaa cagagagagc gctgatttgt taacctgatt ccggtgaaaa
     1501 acgctatctt cacaacctta caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga
     1561 ttaatttcta cccgatgctt gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg
     1621 tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc taacaatgct
     1681 cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa tgggtctgag
     1741 gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga accaaagaat
     1801 ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt
     1861 cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa
     1921 agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga
     1981 aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa ccgttgatgc
     2041 tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg gttaatcagg
     2101 tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa agtgggtatg
     2161 acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga agttgaagca
     2221 aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat tcaggtgacc
     2281 accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt cgctaaagct
     2341 ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga agagttcact
     2401 gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga cgtaactggc
     2461 acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg tacccaggac
     2521 gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca gaaccagact
     2581 ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc
     2641 gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct ggttaaaggt
     2701 gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga
     2761 gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac
     2821 cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt atgcagctgg
     2881 tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa
     2941 accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga gcccgatctg
     3001 gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa
     3061 gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct
     3121 gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg cacagaaact
     3181 gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg aaaacctgtt
     3241 cctggctgcg cgcaacctgc acaaggttga cgtacgcgat gcaactggta tcgacccggt
     3301 tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc aagttgagga
     3361 gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct
     3421 gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac
     3481 gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc
     3541 gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt
     3601 agcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga cttcgttggc
     3661 ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa ccgacatctc
     3721 cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc aaaccttttg
     3781 ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc cgtatcacca
     3841 ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc aaacgcaaca
     3901 aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca
     3961 tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct aaaggcctga
     4021 aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggt aacaccctgc
     4081 cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa ccaggtaaag
     4141 gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgt gatggtgctt
     4201 atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt agaagcagac tgccgtgcaa
     4261 ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg
     4321 cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc
     4381 cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg tggggcgttc
     4441 agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc gtacgtcgcc
     4501 gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg tccttttatt
     4561 gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg
     4621 cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac catcgctgtc
     4681 cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg tcacaaactg
     4741 ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa agcgaagaag
     4801 aaataaggta ggaggaagag atggaaacta tcgctaaaca tcgccatgct cgttcttctg
     4861 ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg
     4921 atattttgac ctacaccaac aagaaagcgg ctgtactggt caagaaagtt ctggaatctg
     4981 ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa gttacgaaaa
     5041 ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag
     5101 atcgcatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc tgagactctg
     5161 gagactagca atgggtcaga aagtacatcc taatggtatt cgcctgggta ttgtaaaacc
     5221 atggaactct acctggtttg cgaacaccaa agaattcgct gacaacctgg acagcgattt
     5281 taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc gtatcgttat
     5341 cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg gtatcgttat
     5401 cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt
     5461 tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa aactggttgc
     5521 tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc
     5581 tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta gcggccgtct
     5641 gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac
     5701 tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg gtgtaatcgg
     5761 cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg ttgaacaacc
     5821 ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc
     5881 gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cacaaaggcc gtaaccgcgg
     5941 tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg
     6001 tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg
     6061 tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa agccgctggc
     6121 agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga ttcagccggg
     6181 taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag cattcaagct
     6241 ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa
     6301 gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca ccgagctgct gaacctgctg
     6361 cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca gtctcacctg
     6421 ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga gaaggcgggt
     6481 gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga
     6541 aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt aaattcatca
     6601 agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc ggtgacgtgg
     6661 ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg gttcgcgttg
     6721 tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg gctcagaaat
     6781 gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg cgccctcgat
     6841 atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt agcggagcac
     6901 taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt
     6961 aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat
     7021 caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa
     7081 ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg tcattcgctt
     7141 cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg gtacgcgtat
     7201 ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta tctctctggc
     7261 accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta
     7321 tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg
     7381 gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg gttccggccc
     7441 tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa
     7501 tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa
     7561 aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta gtacgatggc
     7621 gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg agtttaacta
     7681 caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga
     7741 agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag caatctccgg
     7801 tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg
     7861 ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt tctttgagcg
     7921 actgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt
     7981 cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga
     8041 ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta ctgcgaaatc
     8101 tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca agtaaggtag
     8161 ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt agctttagct
     8221 gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt gaacgcttcc
     8281 gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga ttccagcccg
     8341 tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc
     8401 gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa
     8461 aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat gagcatgcaa
     8521 gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg
     8581 gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt
     8641 tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact tactctgaag
     8701 tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc
     8761 atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt
     8821 tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa
     8881 attatctgct acgtagccta atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc
     8941 gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa
     9001 aacggcgagc tgactcgtac tctcaacgat gctgttgaag ttaaacatgc agataatacc
     9061 ctgaccttcg gtccgcgtga tggttacgca gacggttggg cacaggctgg taccgcgcgt
     9121 gccctgctga actcaatggt tatcggtgtt accgaaggct tcactaagaa gctgcagctg
     9181 gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc
     9241 tctcatcctg ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc gactcagact
     9301 gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc ggatctgcgc
     9361 gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc gttacgccga cgaagtcgtg
     9421 cgtaccaaag aggctaagaa gaagtaaggt aacactatgg ataagaaatc tgctcgtatc
     9481 cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg cctggtggta
     9541 catcgtaccc cgcgtcacat ttacgcacag gtaattgcac cgaacggttc tgaagttctg
     9601 gtagctgctt ctactgtaga aaaagctatc gctgaacaac tgaagtacac cggtaacaaa
     9661 gacgcggctg cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa
     9721 gatgtatcct ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc actggcagat
     9781 gctgcccgtg aagctggcct tcagttctaa ggtagaggtg taagatggct cacatcgaaa
     9841 aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta
     9901 aaggtggtcg tattttctcc ttcacagctc tgactgtagt tggcgatggt aacggtcgcg
     9961 ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa
    10021 aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg cactctgcaa caccctgtta
    10081 aaggtgttca cacgggttct cgcgtattca tgcagccggc ttccgaaggt accggtatca
    10141 tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg ggttcataac gttctggcta
    10201 aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc aactattgat ggcctggaaa
    10261 atatgaattc tccagaaatg gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg
    10321 ggaaataaac catggcaaag actattaaaa ttactcaaac ccgcagtgca atcggtcgtc
    10381 tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg tcgtattggt cacaccgtag
    10441 agcgcgagga tactcctgct attcgcggta tgatcaacgc ggtttccttc atggttaaag
    10501 ttgaggagta agagatgcgt ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg
    10561 gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa aaccggtggt cgtggtcaca
    10621 aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc
    10681 ctctgtaccg tcgtctgccg aaattcggct tcacttctcg taaagcagcg attacagccg
    10741 aagttcgtct gtctgacctg gctaaagtag aaggcggtgt agtagacctg aacacgctga
    10801 aagcggctaa cattatcggt atccagatcg agttcgcgaa agtgatcctg gctggcgaag
    10861 taacgactcc ggtaactgtt cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg
    10921 aagctgctgg cggtaaaatc gaggaataag tagcagatgg ctaaacaacc gggattagat
    10981 tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca gactgctgtt tgttatcggt
    11041 gcgctgattg tgttccgtat tggctctttt attccgatcc ctggtattga tgccgctgta
    11101 cttgccaaac tgcttgagca acagcgaggc accatcattg agatgtttaa catgttctct
    11161 ggtggtgctc tcagccgtgc ttctatcttt gctctgggga tcatgccgta tatttcggcg
    11221 tcgatcatta tccagctgct gacggtggtt cacccaacgt tggcagaaat taagaaagaa
    11281 ggggagtctg gtcgtcgtaa gatcagccag tacacccgct acggtactct ggtgctggca
    11341 atattccagt cgatcggtat tgctaccggt ctgccgaata tgcctggtat gcaaggcctg
    11401 gtgattaacc cgggctttgc attctacttc accgctgttg taagtctggt cacaggaacg
    11461 atgttcctga tgtggttggg cgaacagatc actgaacgag gtatcggcaa cggtatttca
    11521 atcattatct tcgccggtat tgtcgcggga ctcccgccag ccattgccca tactatcgag
    11581 caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt tggttgcagt attagtattt
    11641 gcagtgacgt tctttgttgt atttgttgag cgtggtcaac gccgcattgt ggtaaactac
    11701 gcgaaacgtc agcaaggtcg tcgtgtctat gctgcacaga gcacacattt accgctgaaa
    11761 gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca gtattattct gttcccggcg
    11821 accatcgcgt catggttcgg gggcggtact ggttggaact ggctgacaac aatttcgctg
    11881 tatttgcagc ctgggcaacc gctttatgtg ttactctatg cgtctgcaat catcttcttc
    11941 tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag
    12001 tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa cggcgaagta tatcgataaa
    12061 gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta cctttatctg cctgatcccg
    12121 gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg gtgggacctc actgcttatc
    12181 gttgttgtcg tgattatgga ctttatggct caagtgcaaa ctctgatgat gtccagtcag
    12241 tatgagtctg cattgaagaa ggcgaacctg aaaggctacg gccgataatt ggtcgcccga
    12301 gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc aagaaattat gccgtaactg
    12361 caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa
    12421 acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa agttgggttg agctggctag
    12481 attagccagc caatcttttg tatgtctgtg cgtttccatt tgagtatcct gaaaacgggc
    12541 ttttcagcat ggaacgtaca tattaaatag taggagtgca tagtggcccg tatagcaggc
    12601 attaacattc ctgatcataa gcatgccgta atcgcattaa cttcgattta tggcgtcggc
    12661 aagacccgtt ctaaagccat cctggctgca gcgggtatcg ctgaagatgt taagatcagt
    12721 gagctgtctg aaggacaaat cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa
    12781 ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc tgatggatct tggttgctat
    12841 cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca
    12901 cgtacccgta agggtccgcg caaaccgatc aagaaataat cggggtgatt gaataatggc
    12961 aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca
    13021 tatccatgct tctttcaaca acaccatcgt gactatcact gatcgtcagg gtaacgcgtt
    13081 gggttgggca acagccggtg gttccggttt ccgtggttct cgcaaatcca ctccgtttgc
    13141 agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa gaatacggca tcaagaatct
    13201 ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct actattcgtg ctctgaacgc
    13261 cgcaggtttc cgcatcacta acattactga tgtgactccg atccctcata acggttgtcg
    13321 tccgccgaaa aaacgtcgcg tataacgcct cgttttccag gtttgttgga gaaagaaaat
    13381 ggcaagatat ttgggtccta agctcaagct gagccgtcgt gagggcaccg acttattcct
    13441 taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt gaacaagctc ctggccagca
    13501 cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt
    13561 tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac tactacaaag aagcagcacg
    13621 tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt
    13681 tgtataccgt atgggcttcg gtgccactcg tgcagaagca cgtcagctgg ttagccataa
    13741 agcaattatg gtaaacggtc gtgttgttaa catcgcttct tatcaggtta gtccgaatga
    13801 cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct
    13861 ggctgagcag cgtgaaaagc caacctggct ggaagttgat gctggcaaga tggaaggtac
    13921 gtttaagcgt aagccggagc gttctgatct gtctgcggac attaacgaac acctgatcgt
    13981 cgagctttac tccaagtaaa gcttagtacc aaagagagga cacaatgcag ggttctgtga
    14041 cagagtttct aaaaccgcgc ctggttgata tcgagcaagt gagttcgacg cacgccaagg
    14101 tgacccttga gcctttagag cgtggctttg gccatactct gggtaacgca ctgcgccgta
    14161 ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac
    14221 atgagtacag caccaaagaa ggcgttcagg aagatatcct ggaaatcctg ctcaacctga
    14281 aagggctggc ggtgagagtt cagggcaaag atgaagttat tcttaccttg aataaatctg
    14341 gcattggccc tgtgactgca gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc
    14401 cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag
    14461 ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat tcattcggaa gaagatgagc
    14521 gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc tgtggagcgt attgcctaca
    14581 atgttgaagc agcgcgtgta gaacagcgta ccgacctgga caagctggtc atcgaaatgg
    14641 aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg tgcggcaacc attctggctg
    14701 aacaactgga agctttcgtt gacttacgtg atgtacgtca gcctgaagtg aaagaagaga
    14761 aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga tctggaattg actgtccgct
    14821 ctgctaactg ccttaaagca gaagctatcc actatatcgg tgatctggta caacgtaccg
    14881 aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc tcttactgag attaaagacg
    14941 tgctggcttc ccgtggactg tctctgggca tgcgcctgga aaactggcca ccggcaagca
    15001 tcgctgacga gtaaccggat cacaggttaa ggttttactg agaaggataa ggtcatgcgc
    15061 catcgtaaga gtggtcgtca actgaaccgc aacagcagcc atcgccaggc tatgttccgc
    15121 aatatggcag gttcactggt tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa
    15181 gagctgcgcc gcgtagttga gccgctgatt actcttgcca agactgatag cgttgctaat
    15241 cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg tggcaaaact gtttaatgaa
    15301 ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc gtattctgaa gtgtggcttc
    15361 cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg ttgatcgttc agagaaagca
    15421 gaagctgctg cagagtaatc tgaagcaacg taaaaaaacc cgccccggcg ggttttttta
    15481 tacccgtagc atccccactt atctacaata gctgtactct ttttgttcat cccctggagt
    15541 atttatgtgg ttacttgacc agtgggcaga gcgtcatata gcagaagcgc aagcgaaagg
    15601 tgagtttgat aacctggcag gtagcggcga accattgata ctggatgatg attctcatgt
    15661 gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat gccggttgct taccgccaga
    15721 acttgagcag cggagggaag caattcagct tctggatatc ctgagaggta tccgtcacga
    15781 tgatccgcaa tatcaagagg ttagccgtcg attgtcatta ctggaattga agctgcgaca
    15841 agctggattg agtaccgatt ttttacgcgg cgattatgct gacaagttgt tggacaaaat
    15901 caacgataac tagtggagta tgtatgtatc gcattggtga gctggcaaaa atggcggaag
    15961 taacacccga cacgattcgt tattacgaaa agcagcagat gatggagcat gaagtgcgta
    16021 ccgaaggtgg gtttcgcctg tataccgaaa gcgatctcca gcgattgaaa tttatccgcc
    16081 atgccagaca actaggtttc agtctagagt cgatccgcga gttgctgtcg atccgtatcg
    16141 atcctgaaca ccatacctgt caggagtcaa aaggcattgt gcaggaaaga ttgcaggaag
    16201 tcgaagcacg gatagccgag ttgcagagta tgcagcgttc cttgcaacgc cttaacgatg
    16261 cctgttgtgg gaccgcccat agcagtgttt attgctcgat tcttgaagct cttgaacaag
    16321 gggcgagtgg cgttaagagt ggctgttgat tttttgcact gacaggatta cactcgcgcc
    16381 gttaaataac caactggagt ttttatgagc cgatatcagc atactaaagg gcagataaag
    16441 gataatgcga tagaagcatt actacatgat cccttattcc gacagcgagt agagaaaaat
    16501 aagaaaggga aaggcagtta catgcgaaaa ggaaaacatg gcaatcgggg gaactgggag
    16561 gccagtggca agaaagtaaa tcactttttt accactggcc ttctgctttc aggcgtttgt
    16621 taagagcggt tattctgctc tttcagcaaa tcacgaattt ctgtcagtaa tacttcttct
    16681 ttagttggtg ctggtgcggc tgctggttct tcttttttcc gattcagttt gttgattagc
    16741 ttaatcgcca taaagatggc aaaggccaca atcagaaaat caaagacgtt ttgaatgaag
    16801 acaccgtaat gcatcacaac agcagggata tccccttgcg catcgcgtag cgtgacagca
    16861 aactgtttaa agtcgatccc gccaattaat aagcccagag gtggcatgat gatatcggca
    16921 accagtgaag atacaatctt cccgaatgcc gcaccgataa tgacacccac cgccaaatcc
    16981 accacgttcc cgcgcatcgc aaattcgcga aattctttaa taatgctcat gttattctcc
    17041 ctatgaagcc gacaaccata agtctaacaa atgttaagcc attttcctgc caggaatgaa
    17101 tttaattaat ccttgataat caaagggaaa taataaggcg tcattagacg ccttattaat
    17161 tacaagaaga aagggcttgg ctggaagagt ctttcgacgt cggtaataaa ttttttatct
    17221 gtgaggaaca taattacgtg atcgccttgc tcaatgcgca gattgtcatt ggcaatcatc
    17281 acgtcgtttc cacgtaccac cgctccaata atcgttcctg gcggtagctt gatttcgtca
    17341 atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt gagcaacggc ttcaatagct
    17401 tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat ctgcttttcg cacatggcta
    17461 agcaacgcag aaatagttgc ttgttgtggt gaaatcgcaa tatcgatcac gctcccctga
    17521 accagatcca cataagcgcg acgctggatc aataccatca cctttttcgc acccatacgt
    17581 ttggcaagca tggcggacat gatattggcc tcgtcatcgt tggtgacggc aataaacaga
    17641 tcaacttgat cgatatgttc ttcggccagc agttcttgat ccgacgcatc accaaaaaag
    17701 acgatcgtat tctgtaactt ttccgccagt tcggcagcgc gctgctgatt acgttcgatg
    17761 agtttaacgc tgtaatcttt ttccagacga cgcgccagcc ctgcaccgat attaccgcca
    17821 ccaaccagca tgatccgctt atacggtttt tctagtcgct gtaattcact catcaccgcg
    17881 cggatatgct gtgaagcggc aataaagaac acttcatcac cagcttcaac aatggtcgaa
    17941 ccttgcggac gaatggggcg atcgtggcgg aaaattgctg ccacacgagt atcgatatgt
    18001 ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca gcgggccgcc ataataggct
    18061 ttaaccacag ccaggctgac tttaccctca gcgaagttca ccacctgcaa tgcgccggga
    18121 tactcaatca gtcggtaaat attatcgata accaactgct ctggtgcgat cagatgatca
    18181 atcggtacag catctgaatg aaatagctta tcggcatcgc gcacgtagtc tggtgagcgg
    18241 atacgagcga tgcgattagg ggtgttgaaa agtgagtagg ctacctggca ggcaaccata
    18301 ttggtttcat ctgaactggt tacagcaacc agcatatcgg cgtcgtcggc acctgcctcc
    18361 cgcaatacgc gtggatgaga gccatgcccc tgcacgactc gcaggtcaaa tttatcctgc
    18421 aaggtccgca gacgctcacc gttggtatcg acaacagtga tatcgttgtt ctcgccaacc
    18481 aggttttccg ccagtgtgcc gccaacctgg ccggcaccca gaatgataat tttcatcagt
    18541 cgcgacccgt tatctcatca ctttttgatt agcttagcgt aaaagaagcc gtcgccctct
    18601 tcggcaccag gcagattttg tttacccggt tgctctggtg ttcctgtttc gcaaagttcg
    18661 gcatcagcgg tacgttgcaa aaaggcttta atctgcaggc tattctcttc cggtaacacc
    18721 gaacaggtgg catagaccag agttccaccg gattttaaat gcggccaaat ggcgtcgaga
    18781 atttcagact gcaattgcgc gagttccggg atatcgcgat cgcgacgtaa ccatttaata
    18841 tccggatggc gacgaatcac accggttgct gaacaaggcg catctaataa aatgcgatca
    18901 aactgttgct cgccacacca ttgggaaggg taacggccat caccttgttt cacggttgcc
    18961 ttcataccaa ggcgttttaa attgtcgtaa acgcgagaga ggcgctgttc gtcaatatca
    19021 accgcaacaa cctgcgcttc tggtgccacc tcaaggatat gcgttgtttt accgccgggg
    19081 gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg caagccaggt catgcaacct
    19141 tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa aaccaggtag cgcatgaaca
    19201 ggtgcaggtg tttccagccg tacagcatca gggtaatccg tatgtgggaa gcctttcatt
    19261 cctgcttcat ccagcaatgc aagccagttg tcgcgggaat gatgcgtacg attaatgcgc
    19321 agccacattg gcggacgctg gttattggct tcgacgatgg attgccactg ctctggatac
    19381 gctttttgca gacgcttcag caaccaggaa ggatgcagat aacgagcatc actggcatta
    19441 aactcggcta ataactcttc ttgctgacgc tggaactggc gtaatacacc gtttatcaac
    19501 cctttaagtt gcggacgctt aattgcgata gcgccttcaa ccgtttcagc cagcgcagca
    19561 tgaggtggaa tgcgggtata aagcagttga tacaaaccaa ccataatcag gtaatgcacg
    19621 gtccgctgtt tgccggtcat cggacgggcc attaacttat taatcagcca gtctaactgc
    19681 gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa gtgctttgtc tttatcggaa
    19741 actttttgct ggagcggtgg cagaatgttg cttaatgatt gcccttgctc gacgacttgt
    19801 tcaacggcct gggccgccat gctacgtaaa ttacgttgtt ttttcataag tataaaaatg
    19861 cccggcaaga ccgggcttgg aagagtggac tatcaggcca gacggttgcc cggaacaaac
    19921 cattcccgac gagaattcag gaggtcttgc gcgctcatcg ctttcttacc cgcaggttgt
    19981 aatgagagca ggttcaggat tccatcacca gtcgcaacct gaatgccttg tttgttggct
    20041 tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa tgaccgatgc tttccagact
    20101 ttaaccggct gtccttcaat ttccacccag ctcattggcc atggattgaa agcgcgaatg
    20161 cagcgttcaa gctgtgctgc cgaaagtgac cagtcaatac gcgcttcttc tttactcaac
    20221 ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc
    20281 aattgtttca acgtggtgat aagcccttgt gggccaagct ctgccagctt gtcgtacagc
    20341 gtaccactgg tatcttctgc agtaatcggg caggagagct tatagagcat gtcaccggtg
    20401 tctaaaccaa catccatttg cataatggtc acaccagttt ctgcatcacc cgcccatagt
    20461 gagcgttgga ttggtgcagc accgcgccag cgtggcagca gtgaaccatg aacgttgata
    20521 cagccaagac gcggcatctc cagcactgct ttaggcagaa ttaagccata ggcgacgaca
    20581 accataacat cagcctgtag atcggcgacc agttgctggt tttcttgtgg acgcagggaa
    20641 acaggttgaa aaacgggcag acctttttcc tcagccagaa ctttaaccgg gctgggcatc
    20701 agttttttac cgcgtcctgc cggtcggtct ggctgggtga acacgccaac gacgttatga
    20761 ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata
    20821 atacgtagtg attctgacac gttagttctt atccttaagc ccgggctttc agacgatcca
    20881 gtttttcaac tttctgacga atacgttgtt gtttcagcgg tgacagataa tccataaaca
    20941 gtttgccgac caggtgatcc atctcatgct gaatacagat ggctaacaga ccatctgctt
    21001 ccagttcaaa tggtttaccg tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg
    21061 gcactaaagc acgttgttca gggatcgaca ggcatccttc ttcaatgcct gtttcgccgc
    21121 ttttttctaa aagctccgga ttgattaaca ccagccgttc gtcacggttt tccgaaacat
    21181 caataacaat gatacgttga tggatatcaa cctgggttgc cgccaggcca ataccttctt
    21241 ctgcgtacat cgtctcgaac atatcatcga cgatacgctg aatttctgca ttcacttctt
    21301 ctaccggttt agcaactttg cgaagccgct cgtccggaat atgtaacact tgcaaaactg
    21361 acataaatct ccagagatgt gttcaggagt tagaaagatt atttcttcta ttctagacaa
    21421 atccccctct gattgacagc atcactgacc aatcgcaaag attgctaagg ctgcttatgg
    21481 cagggagata aggatggtcg atacagaaat ttggctgcgt ttaatgagca tcagcagctt
    21541 gtacggcgat gatatggtcc gtatcgctca ctgggtggca aaacagtcgc atattgatgc
    21601 ggttgtattg cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc
    21661 acgaaagagt atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaatccc
    21721 tgcggacagc gaattttatc ctcctcaact tctggcgacg acagattacc ccggcgcact
    21781 gtttgttgaa ggagaactgc acgcgctgca ttcatttcag cttgccgtag tggggagtcg
    21841 ggcgcattca tggtatggcg agcgatgggg acgattattt tgcgaaactc tggcgaagcg
    21901 tggagtgaca attacgagtg gactggcgcg tggaatcgat ggtgtggcgc ataaagcggc
    21961 cttacaggta aatggcgtca gcattgctgt attggggaat ggacttaata ccattcatcc
    22021 gcgtcgccat gcccgactgg ctaccagttt gcttgaacat ggtggggcac ttgtctcgga
    22081 atttcccctc gatgttccac cccttgctta caatttccca cgaagaaatc gcattatcag
    22141 tggtctaagt aaaggtgtac tggtggtgga agcggctttg cgcagtggtt cgctggtgac
    22201 agcacgttgt gcgcttgagc aggggcgtga agtttttgcc ttgccaggtc cattagggaa
    22261 tccgggaagc gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc
    22321 ggaagaaatt ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa
    22381 ttcattttat tcaccagatc aggaagacgt ggcattgcca tttcctgagc tcctggctaa
    22441 cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc
    22501 agaggtagtt actcaactac tcgaactgga gttagcagga tggatcgcag ctgtacccgg
    22561 cggctatgtc cgattgagga gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa
    22621 cctatattca cactgaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta
    22681 ccgacgcagg ttttgagcga gaagatatct acaatgccct gctatggctg gaaaaacttg
    22741 ctgattatca ggaagggttg gcagaaccga tgcaactggc ctcggatcct ctctccatgc
    22801 gtatttatac accggaagag tgtgaacgac tggatgccag ctgccgtggg tttctgcttt
    22861 tccttgagca gattcaggtg ctcaaccttg aaactcgtga aatggtgata gagcgagtgc
    22921 tcgcgctgga taacgcagag ttcgaactgg atgatctgaa atgggtgatc ctgatggtgt
    22981 tgttcaacat cccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag
    23041 tgaatgaagg tatgctgcat taatcatttc tttaattcag cataagttgt tatggcaaaa
    23101 tcagcactgt tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactggtt
    23161 attcgatccg ggaaacacgg tccgtttctt ggatgctcac agtatccggc gtgtgactac
    23221 gtccgtcctc tgaaatcttc agcggatgga catattgtca aagttctgga ggggcaggtt
    23281 tgccccatat gtggcgcaaa tctggtatta cgccagggac gctttggtat gtttattggt
    23341 tgcagtaact accctgaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca
    23401 attacatgcc cccaatgtcg gacgggccat ctggtccagc gccgctcccg ttatggcaaa
    23461 acatttcact cttgtgatcg ctacccggag tgtcaatttg ccattaactt caaacccata
    23521 gctggagaat gccctgagtg tcattatccg ctactcatcg aaaagaaaac cgcgcagggt
    23581 gtaaaacact tttgtgccag taaacaatgt ggaaagccgg tttcggcgga ataataacgt
    23641 gaataataac ctgcaaggag acgctatcgc agctgcgata gatgttctca atgaagaacg
    23701 tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg tgcgatcctg atagcgaaac
    23761 agcagtgatg cgactgttgg agttaaaaca gcgtccggtt gataaggggc tgattttaat
    23821 cgcagcaaat tacgagcagc ttaaacccta tattgatgac accatgttga ctgacgcgca
    23881 gcgtgaaacc attttttccc gctggccagg tccagtcacc tttgtctttc ccgcgcctgc
    23941 gacaacaccg cgctggttga cgggccgctt tgattcgctt gctgtacgag tcaccgacca
    24001 tccgttggtg gctgctttgt gccaggctta tggtaaaccg ctggtttcta ccagtgccaa
    24061 cttgagtgga ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc
    24121 gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc
    24181 cctgacgggt gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta
    24241 atccgatagc ccacagcaaa tcgccattca ttcatcagca atttgctcag caactgaata
    24301 ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga tttcgtcaac acactgaacg
    24361 ctttctttag tgctggtggt aaaggtgcga atgtgacggt gccttttaaa gaagaggctt
    24421 ttgccagagc ggatgagctt actgaacggg cagcgttggc tggtgctgtt aataccctca
    24481 tgcggttaga agatggacgc ctgctgggtg acaataccga tggtgtaggc ttgttaagcg
    24541 atctggaacg tctgtctttt atccgccctg gtttacgtat tctgcttatc ggcgctggtg
    24601 gagcatctcg cggcgtacta ctgccacttc tttccctgga ctgtgcggtg acaatcacta
    24661 atcgaacggt atcccgcgcg gaagagttgg ctaaattgtt tgcgcacact ggcagtattc
    24721 agacgttggg tatggacgaa ctggaaggtc atgagtttga tctcattatt aatgcaacat
    24781 ccagtggcat cagtggtgat attccggcga tcccgtcatc gctcattcat ccaggcattt
    24841 attgctatga catgttctat cagaaaggaa aaactccttt tttggcatgg tgtgagcagc
    24901 gaggctcaaa gcgtactgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct
    24961 tccttctttg gcatggcgtt ctgcctgatg tagaaccagt tataaagcaa ttgcaggagg
    25021 aattgtccgc gtgaatcagg ccatccagtt tccggacaga gaagagtggg tcgagaataa
    25081 aaaatgtgta tgttttcccg ctctcgtgaa tggcatgcaa ctgacatgcg cgatctctgg
    25141 cgagagtctg gcgtatcgct ttactggaga tacgccagaa cagtggttag cgagttttcg
    25201 tcagcatcgc tgggacctgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga
    25261 tgatcaaggc tgggtctggt taccctgatc cagatattcg tccttccatt tcacgtaatt
    25321 attcgcggaa tagcgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac
    25381 gggactaccg agatacagat atccgctctc cagccgttta ttttgtggga ccagactacc
    25441 cgcaccaatc atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc
    25501 aaccaaaact cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac
    25561 atcttcgcca atggttaatg ggttgccatc tgggttgtat gaggatttat gagtgacatg
    25621 caacatactg ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc
    25681 acgaatcaca acgagcggcc agatccccac atcatcagcc agacgaacgt caccaatcac
    25741 gacactgcta tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg
    25801 gcgtaaaaca tcagacatac ttacctcagc aataaatgat ttactaatga ctttgggggc
    25861 attattggcc ttgtgcaagt cttttagtat gcaaaaaagc accgttttgt gtgcgattgc
    25921 agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc aaaaaaatac ttgtgcaaaa
    25981 aattgggatc cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaggatg
    26041 gtcggaacaa cgaagag
//
LOCUS       DAZFKF010000070        25814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFKF010000070 DAZFKF010000000
VERSION     DAZFKF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.070"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(47..127)
                     /gene="shoB"
                     /locus_tag="ACHKA1_003374"
     CDS             complement(47..127)
                     /gene="shoB"
                     /locus_tag="ACHKA1_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO5680981.1"
                     /translation="MTDCRYLIKRAIKIIIAVLQLILLFL"
     gene            complement(548..928)
                     /gene="acpS"
                     /locus_tag="ACHKA1_003375"
     CDS             complement(548..928)
                     /gene="acpS"
                     /locus_tag="ACHKA1_003375"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO5680982.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(928..1659)
                     /gene="pdxJ"
                     /locus_tag="ACHKA1_003376"
     CDS             complement(928..1659)
                     /gene="pdxJ"
                     /locus_tag="ACHKA1_003376"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO5680983.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRNACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1671..2399)
                     /gene="recO"
                     /locus_tag="ACHKA1_003377"
     CDS             complement(1671..2399)
                     /gene="recO"
                     /locus_tag="ACHKA1_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO5680984.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2411..3316)
                     /gene="era"
                     /locus_tag="ACHKA1_003378"
     CDS             complement(2411..3316)
                     /gene="era"
                     /locus_tag="ACHKA1_003378"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO5680985.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3313..3993)
                     /gene="rnc"
                     /locus_tag="ACHKA1_003379"
     CDS             complement(3313..3993)
                     /gene="rnc"
                     /locus_tag="ACHKA1_003379"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO5680986.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4266..5240)
                     /gene="lepB"
                     /locus_tag="ACHKA1_003380"
     CDS             complement(4266..5240)
                     /gene="lepB"
                     /locus_tag="ACHKA1_003380"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO5680987.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5256..7055)
                     /gene="lepA"
                     /locus_tag="ACHKA1_003381"
     CDS             complement(5256..7055)
                     /gene="lepA"
                     /locus_tag="ACHKA1_003381"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO5680988.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7253..7732)
                     /gene="rseC"
                     /locus_tag="ACHKA1_003382"
     CDS             complement(7253..7732)
                     /gene="rseC"
                     /locus_tag="ACHKA1_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO5680989.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7729..8685)
                     /gene="rseB"
                     /locus_tag="ACHKA1_003383"
     CDS             complement(7729..8685)
                     /gene="rseB"
                     /locus_tag="ACHKA1_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO5680990.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8685..9335)
                     /gene="rseA"
                     /locus_tag="ACHKA1_003384"
     CDS             complement(8685..9335)
                     /gene="rseA"
                     /locus_tag="ACHKA1_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO5680991.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9368..9943)
                     /gene="rpoE"
                     /locus_tag="ACHKA1_003385"
     CDS             complement(9368..9943)
                     /gene="rpoE"
                     /locus_tag="ACHKA1_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO5680992.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9940..10095)
                     /gene="rseD"
                     /locus_tag="ACHKA1_003386"
     CDS             complement(9940..10095)
                     /gene="rseD"
                     /locus_tag="ACHKA1_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO5680993.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10351..11973
                     /gene="nadB"
                     /locus_tag="ACHKA1_003387"
     CDS             10351..11973
                     /gene="nadB"
                     /locus_tag="ACHKA1_003387"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO5680994.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERN
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVHGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(11958..12695)
                     /gene="trmN"
                     /locus_tag="ACHKA1_003388"
     CDS             complement(11958..12695)
                     /gene="trmN"
                     /locus_tag="ACHKA1_003388"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO5680995.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12827..14161
                     /gene="srmB"
                     /locus_tag="ACHKA1_003389"
     CDS             12827..14161
                     /gene="srmB"
                     /locus_tag="ACHKA1_003389"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO5680996.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14194..15075)
                     /gene="yfiE"
                     /locus_tag="ACHKA1_003390"
     CDS             complement(14194..15075)
                     /gene="yfiE"
                     /locus_tag="ACHKA1_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5680997.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
     gene            15178..15765
                     /gene="eamB"
                     /locus_tag="ACHKA1_003391"
     CDS             15178..15765
                     /gene="eamB"
                     /locus_tag="ACHKA1_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO5680998.1"
                     /translation="MTPTLLSAFWTYTLITALTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISYSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(15820..16203)
                     /gene="grcA"
                     /locus_tag="ACHKA1_003392"
     CDS             complement(15820..16203)
                     /gene="grcA"
                     /locus_tag="ACHKA1_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO5680999.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            16508..17197
                     /gene="ung"
                     /locus_tag="ACHKA1_003393"
     CDS             16508..17197
                     /gene="ung"
                     /locus_tag="ACHKA1_003393"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO5681000.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            complement(17245..18282)
                     /gene="yfiF"
                     /locus_tag="ACHKA1_003394"
     CDS             complement(17245..18282)
                     /gene="yfiF"
                     /locus_tag="ACHKA1_003394"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO5681001.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            18489..18908
                     /gene="trxC"
                     /locus_tag="ACHKA1_003395"
     CDS             18489..18908
                     /gene="trxC"
                     /locus_tag="ACHKA1_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO5681002.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            18977..19675
                     /gene="tapT"
                     /locus_tag="ACHKA1_003396"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             18977..19675
                     /gene="tapT"
                     /locus_tag="ACHKA1_003396"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO5681003.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAEAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19707..22367
                     /gene="pat"
                     /locus_tag="ACHKA1_003397"
     CDS             19707..22367
                     /gene="pat"
                     /locus_tag="ACHKA1_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO5681004.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            22481..23836
                     /gene="pssA"
                     /locus_tag="ACHKA1_003398"
     CDS             22481..23836
                     /gene="pssA"
                     /locus_tag="ACHKA1_003398"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO5681005.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            23882..24205
                     /gene="yfiM"
                     /locus_tag="ACHKA1_003399"
     CDS             23882..24205
                     /gene="yfiM"
                     /locus_tag="ACHKA1_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO5681006.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(24202..25500)
                     /gene="kgtP"
                     /locus_tag="ACHKA1_003400"
     CDS             complement(24202..25500)
                     /gene="kgtP"
                     /locus_tag="ACHKA1_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO5681007.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(25617..>25814)
                     /locus_tag="ACHKA1_003401"
     CDS             complement(25617..>25814)
                     /locus_tag="ACHKA1_003401"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681008.1"
                     /translation="KRKAILTDGLFALVKKMPDATLRVLYSHICQIQQRIRLPQLAHL
                     HTCSPHQKFILKLLNNHFPAN"
BASE COUNT         6232 a         6828 c         6443 g         6311 t
ORIGIN      
        1 gcgtccgact ggttcaccgg acgcacctta agtacgtttc cttgtgttat aagaacagaa
       61 ggatcagctg taaaacagca atgatgattt tgatggcccg tttaataagg tatcggcaat
      121 cagtcattcg tttttcctta aacaaggaat gcagccgttc agattagcca ttacatctcc
      181 ccaaaactga acgtgcgagt tattgagggt gcatgctgca ctccacacca gagctttgac
      241 gacaccactc gtttcaatgg gggaattctg tggcatggtg taaagcacag caaaatcgcc
      301 aataacgaaa ccaattttaa tgtacttacg aattggcggt cacgttaatc ttgccatcaa
      361 tatcgctttc ttcgtaaagg ctcgagtttt tatgataaag attgcaagtt gcttgtaaaa
      421 gataactacc ctgatccata atcgctgttg ttgagggtgc gtgctgcact aaattaaagt
      481 tacaaagtaa aacccccgtt ccttaccagt tcgggggttt tactttttaa agagaacggt
      541 attattttta actttcaata attaccgtgg cgcaagcata gtggcgctca tcggccagcg
      601 tcacatgcat atttgcaacg cccagctttt ccgccagttt taatgcctcg ccccacagtc
      661 gcagccgtgg cttaccaagc tcatcgttaa atacttcaaa ctgattaaac gccagaccat
      721 tgcggatccc ggtgccaaac gcttttgctg cggcttcttt tacggcaaag cgtttcgcca
      781 gaaaacgcac cggctggtgg tgcgttttcc aaatttccca ttcgttatcg cttaatacgc
      841 ggcgtgccag gcgctcaccg gatcgggcga tcactgcttc tatgcgagcg atctcgacga
      901 tatccgtgcc taaacctaat attgccatta gccacgcgct tccagcatca ggcgcttcat
      961 ttctgccacc gcatctttca gtccggtcat cactgcacga ccaataatgg catgaccgat
     1021 attcagttcg tgcatctcag ggatggcggc aatggctttt acgttgtgat aggtcagacc
     1081 gtgtccggcg ttaactttca aaccgagact agcggcaaag gtcgcggctt tggcgatacg
     1141 cgccaactct tgcgcctgtt cggcgtcagt tttggcatca gcatagcaac cggtgtggat
     1201 ctcgataaac ggtgcgccaa cctctgccgc agctttgatc tgctcttcat cggcgtcgat
     1261 aaacagagaa acctgaatcc cggcatccgc cagacgtttg caggcattgc gcattttttc
     1321 acgctgccct gcaacgtcca ggccgccttc ggttgttact tcctgacgct tttctggtac
     1381 caggcagcaa aaatgcggct tcgtctcaac cgcgatcgcc agcatctctt cggtcaccgc
     1441 catctccaga ttcatgcggg tatccagcgt ctgacgcagg atgcgcacat cgcggtcagt
     1501 aatgtggcgg cgatcttcac gtaaatgcac ggtaatgccg tccgcccccg cctgctcggc
     1561 aataaacgcg gcctgcaccg gatccgggta agcggtaccg cgcgcgttgc gcagcgtagc
     1621 gatatggtca atgttgacgc ctaacagtaa ttcagccatg acaatcctca tcattcataa
     1681 tgtgttttca ccgttcgctt aggcataaat tgccggaaca gttccctgct ctttaaaggt
     1741 ttaccgccaa gatacggctt aagcgccatg cgggtaaagc gtttcgcggc gcgcagtgtg
     1801 tctgcgtcag gaaattcccg tgcgtttaac gcttttaact gccttccggt gaacgtttta
     1861 ttgtcgataa cgacgcttgc gataaaccct ttttcttcgc gataacgata cgtcatggtg
     1921 tcatctaccg gctcgccgct acccgcacaa tgggtaaaat tgacgccata acccagatgc
     1981 ccgagcagtg ccagttcaaa gcggcgcagc gcgggttctg gcgtaccagt gacccctgca
     2041 agagactgaa tgcagtgcaa gtaatcgaaa aagagttcag agaagcgcgt ctcgtattcc
     2101 agtacgcggg agagaagttc gttgatgtac agaccgctgt aaagcgtgat accgcttaat
     2161 ggcagcgcca gcgagacagc ttcagcactg cgcagcgttt tgacttcgcc acgcccgcca
     2221 aaacgtagca agagaggggt gaaaggctgt aatgcacctt tcagggttga gcgtttagag
     2281 cgtgcgcctt tggcaaccag acgcacgcga cccgattcct ccgtgaagac gtccagcatc
     2341 aggctggttt cgctccacgg gcgactatgc aggacaaatg cgcgctgcca gccttccatc
     2401 ggagttactc ttaaagatcg tcaacgtaac cgagactgcg cagtgcgcgt tcgtcgtcgg
     2461 cccaaccgga tttcactttt acccacagct caaggtgaac aggcgcttca aacatttcct
     2521 gcatgtcttt acgcgcttca atcccgatgg ttttgatctt ggcccctttg ttgccaatga
     2581 ccatcttctt ctgcccttca cgctcaacga gaatcaaacc gttgatgtca taaccaccgc
     2641 gttcgttaga gacgaaacgt tcgatctcca cggtcacgga gtacggcagt tcagcgccga
     2701 ggaaacgcat cagtttttcg cggatgattt cagacgccat aaaacgctgt gagcgatcgg
     2761 tgatgtaatc ttccgggaag tgatgagtcg cttcaggtag atgcttacgc acgattgccg
     2821 caatagtgtc aacattcagc ccggtttcgg cagagattgg cacgatatcg aggaagttca
     2881 tctggcttgc cagaaactgc aggtgcggca gcagatcggc tttctcctgc acgttgtcca
     2941 ctttgttcac cgcgaggatt accggcgctt tgccttcgcg cagtttgttg agcaccattt
     3001 cgtcgtccgg cgtccagcgg gtgccttcaa cgacaaaaat caccagctca acatcgccaa
     3061 tagagctgct cgccgctttg ttcatcaggc ggttaatggc gcgtttttct tccatatgca
     3121 ggcccggtgt atcgacgtag atcgcctgat acgcgccttc agtatggatc cccacaatgc
     3181 ggtgacgagt tgtctgcgcc ttgcgggagg tgatggagat tttctgcccc agcagtttgt
     3241 tcaacaatgt ggatttgcca acgttcggac gtccgacgat ggcaataaat ccgcagtaac
     3301 ttttatcgat gctcattcca gctccagttt tttcaacgcc tgttcggcgg cagcctgctc
     3361 agccttacga cggcttgaac ctgtgccaac caccggttca ctcaggccgc tgacctggca
     3421 gtggattgta aattcctgat cgtgcgcttc gccacgtacc tggactacca gataagtcgg
     3481 cagcggcaga tgacgaccct gcaaatattc ttgcaagcgc gttttcggat ctttttgttt
     3541 atcgcctggg ctaatttcgt ccagacgagt ttgataccag ttgaggatta atttctcgac
     3601 ggtttgaata tcactgtcga ggaatacgcc accaattaat gcttcgacgg tgtcggcgag
     3661 aattgactca cgacgaaatc caccgctttt aagttcacct ggtcctaaac gtaagcactc
     3721 gcctaactca aattcgcgcg ccagttccgc cagcgtattg ccacggacca gcgtggcgcg
     3781 catccggctc atatcgcctt catccacacg agggaaacgg tgataaagcg cattggcgat
     3841 aacgtagctc agaatagagt cgcctaaaaa ttctaaacgc tcgttatgtt tgctgctggc
     3901 actacgatga gttaatgcct gctgcaacag ttcctgatga ttaaaagtgt agcccagctt
     3961 ccgttgaagc cgattaatta cgatggggtt catgcgatac caataaataa atgcgtcaac
     4021 aattcagcac acgaaacaga cctgatatac atggctctgc taactgcttc gctgcagttt
     4081 cagtttagcg gtatatgggg accaacgctg tttcgtgtgc cgtggcaacc tggaggtgcc
     4141 aaccttaaac ttcgggggaa tattctatac acaacgacgg gggatgtcgt tagccacggg
     4201 agatttatct cataaataat tcacgttgtc gccataacgg cgacaacgtg aacgaagatg
     4261 agctattaat ggatgccgcc aatgcgactt aagcgcacac ctgtcggcca ttcgccttct
     4321 tgcttatcga agctcatcca gatagccgtt gcccgaccga ccagattcgc ttcaggcaca
     4381 aagccccagt aacggctgtc cgcgctgttg tcgcggttgt cgcccatcat gaagtattgt
     4441 cccggaggaa caatccaggt tgccagttgt tgccctggct gctggtaata catccccacc
     4501 tgatcctgcg caatcggcac tgtcagaatg cggtgcgtca catcacccag tgtctcttta
     4561 cgctcggaaa gacgaattcc attttctttg gtttcgtttt tcggcacttc aaagaatccg
     4621 ctggtcgctt ccccaccatt acggcgtgag aaggtctgaa cgaaatcgct cggttccacg
     4681 tttgagtagg tgaccggcag cgcgttttca cacgcctggc cggaactgca tcccggttga
     4741 atcgtcagct cttttgagac cggatcgtaa gtgactttat cgcccggtaa acccaccgcg
     4801 cgcttgatgt aatcaagctt tggatcttcc ggatatttaa agaccacgat atcgccgcgt
     4861 ttcggatgac cggtttcgat cagcgttttc tggtagatag gatctttaat gccataagca
     4921 aacttctcta ccagaataaa atcaccgatt aacagagtcg gcatcatcga acctgacgga
     4981 atctggaacg gttcataaat aaacgaacgc acaatcaata cgatagccag taccggaaaa
     5041 acagaagcac cggtttccag ccagccaggc ttcggcgcaa cctttttcaa cgttgcttta
     5101 tccagtgagt caccggcagc cgcctgcgcc gctgcctgac gttcccgccg tttaggtgcg
     5161 aaaaagaatt tatccacgca ccataaaatg cccgtcacca gtgtggcaat caccagaatc
     5221 agggcaaaca tattcgccat gccaactcct aagggttatt tgttgtcttt gccgacgtgc
     5281 agaatggcga ggaacgcttc ctgcggcagc tcgacgttac cgatctgctt catgcgtttc
     5341 ttaccttctt tctgcttctg cagcagcttt ttcttacggc tgatatcgcc gccataacat
     5401 ttagccagta cgtttttacg cagctgtttc acggtggatc gcgcaataat gtgcgtacca
     5461 atcgctgcct gaatggcgat atcaaactgc tggcgtggga tcagatcttt catcttctcc
     5521 accaactcgc gaccgcggtt ttgcgaatta tcacggtggg tgatcaacgc cagcgcatca
     5581 acacgttcac cgttgattaa tacgtctaca cgtaccatgt cggacgcctg gaagcgtttg
     5641 aagttgtaat ccaaagacgc atagccacgc gaggttgatt tcagtcggtc gaagaaatca
     5701 agcaccactt ccgccatcgg gatctcgtac gtcagcgcca cctgattacc gtggtaaacc
     5761 atattggtct gcacgccgcg tttttctacg cacagcgtaa taacgttgcc gagatatgcc
     5821 tgcggcagca gcatgtgaca ctctgcaatc ggctcgcgca gttcgtaaat gttatttacc
     5881 gcaggcagct tggatgggct gtcgacgtag ataacttccc ttgacgtggt ttcaacttca
     5941 tacactacgg tcggcgcagt ggtgatcaga tccagatcgt attcacgttc cagacgctcc
     6001 tggatgatct ccatgtgcag caggccaagg aagccgcagc ggaaaccaaa gcccagcgcg
     6061 ctggagcttt ccggctcata gaacagtgag gcatcgttca ggctgagttt acccagcgcg
     6121 tcacggaagg cttcatagtc gtcggaactt accgggaaca gaccggcgta tacctgcggt
     6181 ttgactttct taaagccagg cagcgccttt tctgccggat tacgcgccag cgttaaggta
     6241 tcgccgactg gagcgccgtg gatatcttta atcgcacata cgagccagcc tacttcgcca
     6301 catttcagtt cagtgcggtc aacctgtttc ggcgtgaaga tgcccagacg gtcggcgtta
     6361 taggtctgcc cggtactcat gactttcact ttgtcgccct tacgcagggt gccgttttta
     6421 atacggataa gtgaaacaac gcccaggtag ttgtcgaacc atgagtcgat aattagtgcc
     6481 tgcaacgggc cttccggatc gccttccggc ggcggaatgt cgcgcaccag acgttcgaga
     6541 acgtcctgca cgccaacgcc ggttttcgct gaacagcgca ccgcgtcagt ggcgtcgatg
     6601 ccgacgatat cttcaatttc ttccgccacg cgttcaggat cggctgccgg caggtcaatc
     6661 ttgttcagta ccggcactac ttcgagatcc atctccatgg cggtgtagca gttcgccaga
     6721 gtttgcgctt ccacgccctg cccggcgtcg accaccagca atgcgccttc gcaggccgcc
     6781 agcgaacggg aaacttcata ggagaagtca acgtggcctg gagtgtcgat aaagttaagc
     6841 tgataggttt cgccgtcaga cgctttgtag tccagcgtca cgctttgcgc tttgatggta
     6901 atgccacgct cacgctcaag atccatggaa tcgagaacct gcgcctccat ttcacggtca
     6961 gacaggccac cgcagatctg gataatacgg tcagacagcg tcgatttacc gtggtcaatg
     7021 tgagctatga tcgaaaagtt acgtatattc ttcataaagt atgattattg tgccttaatg
     7081 cccggttaac caggctttta gaagtcgctg ttctgagctt aacgtctgta ttaatagaaa
     7141 cgccgcattc tacactacaa cattgaggcg aggaaatgtt cataccgtat ggattgtggt
     7201 atctggaaac gtcctcgcat ttgttatgca aaatgcaaca aagccagtga aatcactggc
     7261 tcgcgtcttc cgaagatgtt tcaaatcgca caaggccagg cggcaatgcc acgcttaaga
     7321 tgatcggttg ccattctgcc cgggcagcaa acttacgcga gtagccacgc gcaatcagga
     7381 acccgccaat accgccgagg accgcaccac ataatgctgc gacgtcagaa gcaaagagta
     7441 gctgaaatag cgaagcgatg aggaataatc ccaccagcgg cgacatataa accagtaatg
     7501 cggagctaag caggctgcct tcggcgatcc ccaattccac tttttgcccc ggcactaacg
     7561 gttcatcaca gggtacgaca atggtatgcg tggtttgcgg gccaagttta ttcaacacgc
     7621 ggctaccgca accggcgcgt gaagcgcagc tgctgcatga ggctttaaca tcacaactga
     7681 ccagcgcctg cccgttttgc caggagacga cggtagccca ctctttgatc attgcgctgc
     7741 cccgaactta atattctcgg caatgcgttt cgccgtttgc ggcggcagtt caccgacaat
     7801 ggtgatttcg gcgttatcac gtacgcttgt actgacggtt ctgcgtccgg tgcgcaacat
     7861 ctgatcggtg ctcgatggcg tagcgcggtt aacgtttacc gagaagctga ataatccgtc
     7921 ggaatagaga cgtgattcga taggcatgtt gtccatcgtc ggtagcggac gtcgactact
     7981 ggaaacttcg ctaaaaccct gtggcaacca ggttggcgtc cagctgaatt tagctttttc
     8041 acctacagga acagaaagca acggcggcaa atttgccttc gccagcgtct gcatactgct
     8101 gctgatatcc tgattgacgt taaaagcaat cacgcgaaat tgttccagcg tttcaccatc
     8161 gcgatcaaga agatcaaccc gcatcggtaa tttcgattcg gtgtccatcc acacgatgta
     8221 gctgtagcgt gtgccatcac gagcaaccac gcgaataact tcacaaagac gatcagcaat
     8281 acgcgtgcgc ccgacggaga taaagtcgta gtaaggagaa aggcgtttga aatcggtata
     8341 gatgagcgat ggcagagaat caacaatgta atcgccatta agagtaaacg gttcaaggcc
     8401 cggttcaaaa tagctgattt cattgccgcg ctgtaccact tcccggcgcg ggccatccat
     8461 ttgcaacaat tgtgcaagag gacggttatc gaggcgtgca tgtcgataac gcagagactc
     8521 aacaccctgt ttattgatgc tgatgaatga cagctcgtaa ttcagtgact gactggccag
     8581 gttcatctgc tgtaataacg ccccggacgc gggagtggcc gaggcgttag cagagaataa
     8641 caggctacct gtcactaatg acatggcaaa ccaaagttgc ttcattactg cgattgcgtt
     8701 cctaaagttt gaattcctgg cacctgtaca gcggcttgct gggtttgcgc ctgctcaaac
     8761 tgaagctgtt cagagtggag tcggcgttgc agttcgtaat cctgcaacat tgcattaatg
     8821 cgacgacgct gctcctgtac ctgctgctgt tgaccattat ttgcggtcgc ttcagaaggt
     8881 actcccaggc taaccgggct ggctttaccc atcatcggca gtgtattaaa taccggtgtt
     8941 tcgggctgct gggacgtttc agattgtcca ttatagtgct ggacgccaac gataactgca
     9001 agcgatacgc atgcggctac gcccatttgg gtaagctgtg ccgcccacgg acgtactttc
     9061 tgccagaatg gcattttctg ccattgatgc ggcgcaggct gggcttccgg gatcaatgtc
     9121 gccggttgac gtactggctc ttcttcaatg gcggccatca cgcgtgaaga gatatcgaaa
     9181 tggagcacct cgggagtatc accccgcatt gagtcacgga ttaagtgata gctttcccag
     9241 gttttctgca tttctgggtt atgagccagt tcgttaagca gctcactatc cagcgtttcg
     9301 ccatccatta aagcggaaag ttgttctttc tgcatgccta atacccttat ccagtatccc
     9361 gctatcgtca acgcctgata agcggttgaa ctttgttatc aatagcttcc ctcgctcgga
     9421 agatacgtga acgcaccgta cctaccggac aatccatgat agcggctatc tcttcatagc
     9481 tcaggccatc cagctcccgc aaggttattg ccatgcgtaa atcttccggg agggactcaa
     9541 tagttcggaa aactatctgt ctcagttctt ctgacaacat taagttctca gggttcgaaa
     9601 tttctttcaa cgcgccgcca ctttcgaagt tttcagcttc aatggcatcc acatcactgg
     9661 aaggtggacg acgcccctga gcaaccaggt aatttttcgc tgtatttaca gcaatccgat
     9721 acagccatgt ataaaaagcg ctatctcccc ggaacgaatc cagcgcacga taggctttaa
     9781 taaaagcttc ttgtaccaca tcgggaacat cacccgacgg cacatagcgg gaaaccagac
     9841 tcgccacttt atgctgatag cgcactacca gtaagttaaa ggctttctga tctcccttct
     9901 ggacccgttc aaccaggacc tggtccgtta actgctcgct catccgaggt aaagtctccc
     9961 caaaccaaat ttccacgcgc tatcgaaacg ccactccatt agctgcaatt tgagcaagca
    10021 aagggttaga gtgtctcgtt tttgtaaagt tccgtaacgc atctgttttt gtttgtcatg
    10081 ctgtagacgg atcattatct atcattataa gtctacagaa tctgaacatc gcattatctg
    10141 tgtagaaatg cccatttaac tgcctgaaga gtaacccaac ggccttttta tttcaccacc
    10201 taatcctcca ccagccagta acttctcttt ttctcgccgc cctgcgtcag cgtgtttagc
    10261 cactgtaaca aatattaaaa tagcaggtgt ttattcgcac aacatgatgc tatgctgacc
    10321 aaaccgtgtt tagtaaatta aacaaagaaa atgaatactc tccctgaaca ttcatgtgac
    10381 gtgttgatta tcggtagcgg cgcagccggg ctttcactgg cgctacgcct ggctgaccag
    10441 catcaggtca tcgttctaag taaaggcccg gtaacggaag gttcaacatt ttatgcccag
    10501 ggcggtattg ccgccgtgtt tgatgaaact gacagcattg actcgcatgt ggaagacaca
    10561 ttgattgccg gggctggtat ttgcgatcgc catgcagttg aatttgtcgc cagcaatgca
    10621 cgatcctgtg tgcaatggct aatcgaccag ggggtgttat ttgataccca cattcagccg
    10681 aatggcgaag aaagctacca tctgacccgt gaaggtggac atagtcaccg tcgtattctt
    10741 catgccgccg acgcaactgg tagagaagta gaaaccacgc tggtgagcaa ggcgctgaac
    10801 catccgaata ttcgcgtgct ggagcgcaac aacgcggttg atctgattat ttctgacaaa
    10861 attggcctgc cgggcacgcg acgggttgtt ggcgcgtggg tatggaaccg aaataaagaa
    10921 acagtggaaa cctgccacgc aaaagcggtg gtactggcaa ccggcggtgc gtcgaaggtt
    10981 tatcagtaca ccaccaatcc ggatatttct tctggcgatg gcattgctat ggcgtggcgc
    11041 gcaggctgcc gggttgcaaa tctcgaattt aatcagttcc accctaccgc gctgtatcac
    11101 ccacaggcac gcaatttcct gttaacggaa gcactgcgcg gcgaaggcgc ttatctcaag
    11161 cgcccggatg gcacgcgttt tatgcccgat tttgatgtgc acggcgaact ggccccgcgc
    11221 gatattgtcg cccgcgccat tgaccatgaa atgaaacgcc tcggcgcgga ttgtatgttc
    11281 cttgatatca gccataagcc cgccgatttt attcgccagc atttcccgat gatttatgaa
    11341 aaattgctcg ggctggggat tgatctcaca caagaaccgg taccgattgt gcctgctgca
    11401 cattatacct gcggtggtgt aatggttgat gatcatgggc gtacggacgt cgagggtttg
    11461 tatgccattg gcgaggtgag ttataccggc ttacacggcg ctaaccgcat ggcctcgaat
    11521 tcattgctgg agtgtctggt ctatggctgg tcggcggcag aagacatcac cagacgtatg
    11581 ccttatgccc acggcgtcag tacgttacca ccgtgggatg aaagccgcgt tgagaaccct
    11641 gacgaacggg tagtaattca gcataactgg cacgagctac gtctgtttat gtgggattac
    11701 gttggcattg tgcgcacaac gaagcgcctg gaacgtgccc tgcggcggat aaccatgctc
    11761 caacaagaaa tagacgaata ttacgcccat ttccgcgtct caaataattt gctggagctg
    11821 cgtaatctgg tacaggttgc cgagttgatt gttcgctgtg cgatgatgcg caaggagagt
    11881 cgtggcctgc attttacgct ggattacccg gaactgctca cgcattccgg tccatcgatc
    11941 ctttctcccg gcaatcatta cataaacaga taaaaagcct gggttagcgc cgtatacgct
    12001 tcggaatagt tctggtctgg cccacgaatg actaagcgat cgctaaagca ttctcccgcc
    12061 tgcggggaga atgccagcag cacccgatgc ggcagtcgcg cttcgttttc cgccacatcc
    12121 gtccgcaaac gtaaatgcca gcccatactt aatgccagtt cagtaaaacc attaccaatc
    12181 tgctctggca gcaccacgca gaaaaatccc tcttcggtaa tgcactccgc cgcacaggtc
    12241 agcaacgatg ggtgatcaag cgtagtagta tagcgagcct gttcccgttg aggtgtcgag
    12301 cactctactc cctgctgata gtaaggtggg ttactgatga ttaaatcgaa gcgtactgtc
    12361 tgctgtgtga tccactgctg aatatccgcc gtatggacgt taatccgctc tgcccacggg
    12421 gactggttga tattttcctg cgcctgcgcg gcagcttcac tttccagttc aactgcatca
    12481 atcatcacgc tgtcatcggt tcgctgcgcc agcattaatg ccagcaaccc gctccccgcg
    12541 ccgatatcaa ggcaacgttt taccccagcc accggtgccc atgcgcccaa taaaatacca
    12601 tccgttccca ctttcatcgc acagcgatcg tgagcaacaa aaaactgttt aaaagtaaat
    12661 ccattacgac gaagcacgga tgtagactgt gacatgaaaa taaaaccttg caggaaaaac
    12721 ggcgatagca ccgggtgaga acaatacccg agaagcgata tccatacaaa cagatgaaga
    12781 ttgcagccgt aacgtctata atcagcgccc cacacagagg tagaacatga ctgtaacgac
    12841 tttttccgaa cttgaactcg acgaaagcct gctggaagcc ctccaggata aaggtttcac
    12901 tcgcccgacc gccattcagg ctgccgccat tccgcctgcg ctcgatggcc gtgatgtact
    12961 cggttctgcg ccgacaggca ccggtaaaac ggcggcgtat ctgctgccag cgttgcagca
    13021 cctgctcgat ttcccgcgta agaaatccgg tccgccgcgt attttgatcc tcaccccaac
    13081 tcgcgagctg gcgatgcagg tggccgatca tgcccgcgaa ctggcgaaac atacgcatct
    13141 ggatatcgcc accatcaccg gcggcgtagc ctatatgaac cacgcggaag tgttcagcga
    13201 aaatcaggac atcgtggtcg ccacgaccgg acgtctgctg caatacataa aagaagagaa
    13261 cttcgattgc cgcgcggttg aaacgctgat cctcgacgaa gcagaccgta tgctggatat
    13321 gggcttcgcc caggatattg aacatattgc tggcgaaacg cgctggcgta aacagaccct
    13381 gctcttttcg gcaacgctgg aaggcgatgc gattcaagac tttgccgagc gtctgctgga
    13441 agatccggtg gaagtttctg ctaatccttc cactcgtgag cgcaaaaaaa ttcatcagtg
    13501 gtattaccgc gccgatgacc tggaacataa aaccgcattg ctggtgcatc tgttaaaaca
    13561 gccggaagcg acccgctcaa ttgtgtttgt gcgtaagcgt gagcgtgtgc atgagctggc
    13621 aaactggctg cgcgaagcgg gcatcaacaa ctgctatctc gaaggtgaga tggtacaagg
    13681 caagcgtaac gaagcgatca agcgtttgac cgaaggtcgc gtaaacgtac tggttgcaac
    13741 cgatgttgct gcgcgcggta tcgacattcc tgacgtcagc cacgtcttta acttcgatat
    13801 gccgcgcagt ggcgatactt atttgcaccg tatcggacgt accgcgcgcg ccggtcgtaa
    13861 aggcaccgca atttcgctgg tagaagccca tgaccatctg ctgctgggca aagttggtcg
    13921 ctatattgaa gagccaatta aagctcgcgt aattgatgag ttacgcccga aaacgcgtgc
    13981 accaagcgag aagcagaccg gcaagccatc gaagaaagta ctggcaaaac gcgccgagaa
    14041 gaaaaaagct aaagagaaag agaagccgcg ggtgaaaaaa cgccatcgcg ataccaaaaa
    14101 tattggtaag cgccgtaaac caagcggaac gggcgtgcca ccgcaaacga cagaagagta
    14161 atctcaatgc cgggtttaag cccggcatta tttttatccc ggcaaaaaac aatcttcaac
    14221 acactgaata aaagtgtgca tcgccgggct aaccgctttt ccggcatggt gagcgcacat
    14281 tgccgtaatg gtctgcgagt gttcgccaaa aggcaattca attaattctc cgctctcaag
    14341 ctctttttcc accgcaaagc ggggcaagta actgacaccg atattcgccg cgacacaacg
    14401 cttgatactt tcaatgctta acagctcaat ggtgttttcc accgtgatcc gtcgctggcg
    14461 cagcgtgctc tcaaatatct gccggaagac acattgcggt tcgttgataa taaagctaca
    14521 ggcgttatgt cttcccggct cagtaaaatc gacatctgca atttgtggtg aagccaccag
    14581 caccagcgat tgttcaccca actctcgtcg gttcagggca tcatcattcc ctacgcgata
    14641 aaagacgccg acatcggcct catcattcag tagcgcatca cgaatcacgt aacagttcag
    14701 cgactgcaac gataaacgca cttttggtgc gcgttgccga aaacgctgca acacctgcgg
    14761 cattcggtaa gagagtaacg tttcacccga aacaacgcgt agttccccgt ccggatccga
    14821 ctcctttttg gcagcctcac gaagggtatc catcactctg gtaagttcat agatgtgcgg
    14881 cagcagcttt ttcccttcgc gggtaaggca cattcgtcga ccaattttct caaataactg
    14941 tactgagaac tcctgctcaa gctgctgaat atgaaaagtc actgtcgatt gtgtgcagca
    15001 caatttttgc gaagctcgca aaaaggaacc ctcttccacc acggttttaa gcgtaataaa
    15061 acggcgcaga tccataaccc caaacctatc gaaaatatcg aatttagaat gtaaaaacat
    15121 tcattttttt aaatattccg tgtcgggtac tgtctaccaa aacagaggag ataacaagtg
    15181 acaccgacgc ttttaagtgc tttttggacc tacaccctga ttaccgccct gacgccagga
    15241 ccgaacaata ttctcgctct tagctcagcc actacgcacg gctttcgtca gagtacccgc
    15301 gtactggcag ggatgagtct gggatttttg attgtcatgc tgctgtgtgc aggcatttcg
    15361 tattcactgg cagtgattga tccggcagcg gtacaccttt tgagttgggc aggagcggca
    15421 tacattgtct ggctggcatg gaaaatcgct accagtccaa caaaggaaga cgggcttcag
    15481 acaaaaccga tcagtttttg ggccagcttt gctttacagt ttgtgaacgt caaaatcatt
    15541 ttgtacggcg ttacggcact ttcgacgttt gtcctaccgc aaacgcaggc gttaagctgg
    15601 gtagttggcg tcagcgtttt gctggcgatg attgggacgt ttggtaatgt gtgctgggcg
    15661 ctggcggggc atctgtttca gcgattgttt cgccagtatg gtcgccagtt aaatatcgtg
    15721 ctcgcgctgc tgctgatcta ttgcgcggta cgcattttct attaattaaa aaagcggaag
    15781 aagtcgccct cttccgctta gtaacttgct acttaagcct tacaggcttt cagtaaaggt
    15841 acgagcaata acgtcgcgct gctgttccgg agtcagagag ttaaagcgaa ctgcatatcc
    15901 ggatacacgg atggtcagct gcggatattt ttccggatgc ttaactgcat cttccagagt
    15961 ttcacgacgc agaacgttaa cgttcaggtg ttggccacct tcaacgcgaa cttctggttt
    16021 cacttctact ggaacttcac ggtactcaat atcacccagt ttgcttactg caaccacttc
    16081 gtcttctgca aaacctgctt tcgcaacgat gcaacgcgct tcgccttttt cgctgtccag
    16141 cagccagaaa gagttcagca gatcgtcgtt agcggcttta gtaatctgga tacctgtaat
    16201 catgtgatgc ctccccggca aaattatttg atttgttcag cctgtcgcgg ccaattggta
    16261 aaaccattgt tgcttgagtg tatatatact cctcaaacac ccttgaatct ttgatttaaa
    16321 tcaataaaaa ccacacatca agtgtggtcg caaatggatt ttattgtttt acatcaactt
    16381 atgcgggtgt gaaattttac caatttacat ttttttgcac tcgtttaagt ctaaaaaatg
    16441 agcatgattt tgttctgtag aaagaagcag ttaagctagg cggattgaag attcgcagga
    16501 gagcgagatg gctaacgaat taacctggca tgacgtgctg gctgaagaga agcagcaacc
    16561 ctattttctt aatacccttc agaccgtcgc cagcgagcgg cagtccggcg tcactatcta
    16621 tccaccacaa aaagatgtct ttaacgcgtt ccgctttacc gagttgggtg acgttaaagt
    16681 ggtgattctc ggccaggatc cttatcacgg accgggacag gcgcatgggc tggcattttc
    16741 cgttcgcccc ggaatcgcca ctcctccgtc actgctgaat atgtataaag agctggaaaa
    16801 tactattccg ggcttcaccc gccctaatca tggttatctt gaaagctggg cgcgtcaggg
    16861 cgttctgcta ctcaatactg tgttgacggt acgcgcaggt caggcgcatt cccacgccag
    16921 cctcggctgg gaaaccttca ccgataaagt aatcagcctg attaaccagc atcgcgaagg
    16981 cgtggtgttt ttgttgtggg gatcgcatgc gcaaaagaaa ggggcgatta tagataagca
    17041 acgccatcac gtactgaaag caccgcatcc gtcaccgctt tctgcgcatc gtggattctt
    17101 tggctgtaac cattttgtgc tggcaaatca gtggctggaa caacatggcg agacgccgat
    17161 tgactggatg ccagtattac cggcagagag tgagtaaatt tgcgggaaaa tgccggatgg
    17221 cagagttgcc acccggctga tttatcaggc tttattctga cgccaccatt caccaagcaa
    17281 aacgcctgtt gcaacagaga tattcagccc ggcaacgttg cccgtaccgt caatcttcac
    17341 gcgcagatcg ttcggatcgc gtgcggcatc cggtaaccct tcatattcct gacccagcac
    17401 cagtaccatt ttcgctggca ggctggtttt gaacagcggt ttaccctgct cgctggaagt
    17461 tgtcactacg gtgtaacccg cctgacggaa atcatccagc acgttaacaa tgttgtcgcc
    17521 agtaatcggc tgaacgtgtt ctgcgccgcc ttctgcggta cggatagccg cacccgactc
    17581 cagcagtgcc gcatcctgca ccacaacgcc tttcacgccg aagtgcgcgc agctgcgcat
    17641 catgccaccc aggttatgcg ggttagattc gttttccagt gccagaacgc aatcctgcgc
    17701 acctgcctgg cttacccact gctgcacggt tgtgccatta cgctttttga tcaggaagca
    17761 aacgccgccg tgatgttccg tgcctgacgc ttttgtcaat tccgcttcat ccaccacatg
    17821 gtacgctttg cggtttgccg ccatccagcg caaggcttct ttaaaacgtg gcgttacgct
    17881 ctggataaac caggcgcgaa caatcgcttc cggacggctc tggaacagtg cctgacatgc
    17941 attttcgccg tagacgcggg tttcttccgc acgctgacga cgcaacactt ccggatcaat
    18001 aaaactttta ccactgatac caccgtgatc ggccttttcc ggcgtctcat cacccggcgc
    18061 gcgggaaacc gtgcgccacg gcgactcttc ccacttgcgg tcacggggct gactctgttt
    18121 gtcatcgcgg gcggggcgac ggccaccgtc agcacgagat tttcctggac gcccgccccc
    18181 tttcccggta cgcgggttgt gggtacgttt atcagaatca tcatcactgc ggacatacat
    18241 cactttgacc ttgccgcttt tacctttcat ttcatcgttc atgcttttct ccaccagcgc
    18301 tgcgcgaagc gcgcagatta cccgaagtcc gcgcggttcg ccatgatttc gtaccaaagc
    18361 ctgcgactat catacctatt gaataaaaca gattgttgtc tggaacaatg tccccgataa
    18421 tatgtaacat attagaaaca taccggcgtc gttgccgata agtctcctta ctcatcccga
    18481 ggttagttat gaataccgtt tgtacccatt gtcaggccat caatcgcatt cccgacgatc
    18541 ggatcgaaga tgcggcaaaa tgcggacgct gcggtcacga cttgtttgac ggagaggtga
    18601 ttaatgcgac cggtgaaacg ctcgacaaat tgctgaaaga tgatctacca gtggtgatcg
    18661 acttctgggc accgtggtgc ggcccctgcc gtaatttcgc accaattttt gaagatgtcg
    18721 cgcaagagcg tagcggtaaa gtgcgctttg tgaaagtgaa taccgaagct gaacgtgaat
    18781 taagcagccg ctttggtatt cgcagtattc cgacgatcat gattttcaaa aacggtcagg
    18841 ttgtcgacat gcttaatggc gcagtgccga aagcgccgtt cgacagctgg ttgaacgaat
    18901 ctctttaatc ttaccggggc gcatcttgtg ccccgttttc tcctctgcga caatggcgtt
    18961 ttttcgacgc tctcttatga ccgaaaacgc tgttctccag ttacgcgccg aacgtattgc
    19021 gcgcgcaaca cgtccttttc ttgcccgcgg taatcgcgtt cgtcgctgcc aacgctgtct
    19081 tttgccagag aaattatgtc tctgttcaac aatcacgcca gcggaagcaa aaagtcgctt
    19141 ctgtttgctg atgttcgaca ccgagccaat gaagcccagt aataccgggc gtctgattgc
    19201 tgatattttg cctgataccg ttgcgtttca atggtcgcgt accgaaccct cgcaggattt
    19261 gctggatctg gtacaaaacc cggattatca gccaatggtg gtctttcccg cctcgtatgc
    19321 tgatgagcaa cgggaagtga tcttcacacc acctgccggt aagccaccgc tgtttatcat
    19381 gctcgatggt acctggccgg aagctcgcaa gatgtttcgt aaaagtccgt atctggataa
    19441 tcttcccgtc atttccgtcg atctttcccg gctttctgcc tatcgcctgc gtgaagccca
    19501 ggctgaaggc caatattgta ctgccgaggt agccatcgca ctgttagata tggccggcga
    19561 taccggggcg gcagcagggt taggcgagca ttttacccgc tttaaaacac gctatctggc
    19621 aggaaaaacg caacatctgg gtagcatcac agcagaacag ttagaaagcg tttaaaatca
    19681 ttcggtcact tctgcgggag accggtatga gtcagcgagg actggaagca ctactgcgac
    19741 caaaatcgat agcggtaatt ggcgcgtcga tgaaacccaa tcgcgcaggt tacctgatga
    19801 tgcgtaacct gctggcggga ggctttaacg gaccggtact cccggtgacg ccagcctgga
    19861 aagcggtgtt gggtgtgttg gcctggccgg atattgccag cttgcccttt acacccgacc
    19921 ttgcggtttt atgtaccaat gccagccgta atcttgctct tctggaagag ctcggcgaga
    19981 aaggctgtaa aacctgcatt attctttccg ccccggcatc gcaacacgaa gatctccgcg
    20041 cctgcgccct gcgccataac atgcgcctgc ttggaccaaa cagtctgggt ttactggctc
    20101 cctggcaagg tctgaatgcc agcttttcgc cggtacctat taaacgcggc aagctggcgt
    20161 ttatttcaca atcggctgcc gtctccaaca ccatcctcga ctgggcgcaa cagcgtgaga
    20221 tgggcttttc ctactttatt gcgctcggcg acagcctgga tatcgacgtt gatgaattgc
    20281 ttgactatct ggcacgcgac agtaaaacca gcgccatcct gctctatctc gaacagttaa
    20341 gcgacgcgcg acgctttgtt tcggcggccc gtagtgcctc gcgtaataaa ccgattctgg
    20401 tgattaaaag cggacgtagc ccggcggcac agcgactgct caacacgacg gcaggaatgg
    20461 acccggcatg ggatgcggct attcagcgtg ccggtttgtt gcgggtacag gacacccacg
    20521 aactgttttc ggcggtagaa acccttagcc atatgcgccc gctgcgtggc gaccggttga
    20581 tgattatcag caacggtgct gcgcctgccg cgctggcgct ggatgcctta tggtcacgca
    20641 atggcaagct ggcaacacta agcgaagaaa cctgccagaa actgcgcgat gcactgccag
    20701 gacatgtggc aatctctaac ccgctcgatc tgcgcgatga cgccagcagc gagcattatg
    20761 tcaaaacgct ggacattctg ctccatagcc aggattttga tgcgctgatg gttattcatt
    20821 cgcccagcgc cgctgctccc gcaacagaaa gtgcgcaagc attaattgaa gcggtaaagc
    20881 atcatccccg cagcaagtat gtttctctgc tgacgaactg gtgcggcgag cactcctcgc
    20941 aagaggcacg acgtttattc agcgaagccg ggctgccgac ctaccgtacc ccggaaggaa
    21001 ccatcactgc ttttatgcat atggtggagt accggcgtaa tcagaagcaa ctacgcgaaa
    21061 cgccggcgtt gcccagcaat ctgacttcca ataccgcaaa agcgcatctt ctattgcaac
    21121 aggcgattgc cgaaggggct acgtcgctcg atacccatga agttcagccc atcctgcaag
    21181 cgtatggcat gaacacgctc cctacctgga ttgccagtga tagcaccgaa gcggtgcata
    21241 ttgccgaaca gattggttat ccggtggcgc tgaaattgcg ttcgccggat attccacata
    21301 aatcggaagt tcagggcgtc atgctgtacc tgcgtacagc caatgaagtc cagcaagcgg
    21361 caaacgctat tttcgatcgc gtaaaaatga cctggccgca ggcgcgggtc cacggcctgt
    21421 tggtgcaaag tatggctaac cgtgctggcg ctcaggagtt gcgggttgtg gttgagcacg
    21481 atccggtttt tgggccgttg atcatgctgg gtgaaggcgg tgtggagtgg cgtcctgaag
    21541 atcaagccgt cgtcgcactg ccgccgctga atatgaacct ggcccgctat ctggttattc
    21601 aggggatcaa aagtaaaaag attcgtgcgc gcagtgcgct acgcccattg gatgttgcag
    21661 gcttgagcca gcttctggtg caggtttcca acttgattgt cgattgcccg gaaattcagc
    21721 gtctggatat tcatcctttg ctggcttctg gcagtgaatt taccgcgctg gatgtcacgc
    21781 tggatatcgc gccgtttgaa ggcgataacg agagtcggct ggcagtgcgc ccttatccgc
    21841 atcagctgga agaatgggta gaattgaaaa acggtgaacg ctgcttgttc cgcccgattt
    21901 tgccagaaga tgagccacaa cttcagcaat tcatttcgcg agtcaccaaa gaagatcttt
    21961 attaccgcta ctttagcgag atcaacgaat ttacccatga agatttagcc aacatgacgc
    22021 agatcgacta cgatcgggaa atggcgtttg tagcggtacg acgtattgat caaacggaag
    22081 agatcctcgg cgtcacgcgt gcgatctccg accctgataa catcgatgcc gaatttgccg
    22141 tgctggttcg ctcggatctc aaagggttag gcttaggtcg acgcttaatg gaaaagttga
    22201 ttacctatac gcgagatcac ggactacaac gtctgaatgg tattacgatg ccaaacaatc
    22261 gtggcatggt ggcgctggcc cgcaagctcg ggtttaacgt tgatatccag ctcgaagagg
    22321 ggatcgttgg gcttacgcta aatcttgccc agcgcgagga atcatgagta aggtactgga
    22381 aatgttgacc acttaatcgg gactggtgtt attattgccc gcttatgtcg tctgcattgc
    22441 acagaggacc cttcaatgaa cagagaagaa atgcactgtg atgttgtcaa aatttaagcg
    22501 taataaacat caacaacacc ttgcccaact acccaagatt tctcaatcag ttgatgatgt
    22561 cgatttcttt tacgctcccg ccgacttccg ggagacgctg ctggaaaaaa tagccagcgc
    22621 gaagcagcgc atttgcattg tcgccctgta tctcgaacag gatgacggtg gcaaaggcat
    22681 tctgaacgcg ttgtatgaag ctaaaaggca gcgtccggaa ctggatgtgc gggtgctggt
    22741 cgactggcat cgtgcacaac gtggacgcat tggcgctgcg gcatctaaca ctaacgctga
    22801 ctggtactgc cgcatggcgc aggaaaatcc gggcgtagat gttccagttt atggcgttcc
    22861 aatcaatacc cgtgaagccc ttggcgttct gcactttaaa ggctttatca tcgacgatag
    22921 cgtactttat agcggtgcca gcctgaacga tgtttacctg catcagcacg ataaatatcg
    22981 ctacgaccgt tatcacctta tccgtaaccg taagatgtca gacattatgt ttgaatgggt
    23041 tacacagaat attatgaatg gccgcggcgt taatcgtctg gatgatgtta atcggccaaa
    23101 aagcccggaa atcaagaacg atattcgtct gttccgccag gagctgcgtg atgccgctta
    23161 tcatttccag ggcgatgccg acaacgatca gctttccgta acgccgctag tggggctggg
    23221 gaaatcgagt ctgttgaaca agaccatttt ccatcttatg ccttgtgccg agcagaaact
    23281 aaccatctgt acgccatact tcaacctgcc agcaatcctt gtgcgcaata ttatccagtt
    23341 gctgcgcgaa gggaaaaagg tcgaaattat tgttggtgat aaaaccgcga acgacttcta
    23401 cattccggaa gatgaacctt tcaagataat tggcgcattg ccttatctct atgagatcaa
    23461 tctgcgtcgt ttcctgagcc gtttgcagta ttacgtcaat actgaccagc tagtggttcg
    23521 gttatggaaa gatgacgaca acacctatca cctaaaaggg atgtgggttg atgataagtg
    23581 gatgttgatc accggtaata acctgaaccc gcgcgcctgg cgtctggatc tggaaaacgc
    23641 cattttgatc cacgatccac aactggagct tgcgccgcag cgagagaaag aactggagct
    23701 gatccgcgag cataccacca tcgttaagca ctatcgcgat ctgcaaagta ttgccgatta
    23761 tccggtgaag gttcgtaaac tcatccgccg tttgcgccgt atccgcatcg accgattaat
    23821 tagccgcatc ctgtaatcac aaccccgtcc tgtacggggt ttgttttttg gaggccacgt
    23881 tttgcgtatt ctttttgtct gttcgctatt gttactttct ggatgcagcc atatggctaa
    23941 cgatagctgg agcgggcagg ataaagctca acactttatc gcctcggcga tgctttccgc
    24001 cgccggaaat gaatattcac agcatcaggg gatgagccgg gatcgcagtg ccatgtttgg
    24061 attgatgttc tctgtcagtt tgggggcgtc aaaagagctt tgggatagcc gccccgaagg
    24121 gagcggctgg agctggaagg atttggcctg ggatgtcgcc ggtgcaagca ccggctatac
    24181 cgtctggcaa ctgacccgtc actaaagacg catccccttc cctttgcgat gtagcatcaa
    24241 agaaaccaga aacgccacca cggccatcaa ggtcacatac cagaagaagg ctgtttccat
    24301 tcctattgat ttcagcgaca acgctacgta ctccgccgaa ccaccaaata tagcattagc
    24361 gaccgcatat gacagaccaa cgcctaatgc gcgaacctgt gccgggaaca tctcagcctt
    24421 cagtattcca ctgattgatg tataaaaact cactatcagc agggcacaca tcaccagacc
    24481 aaaagcggca taaggcgagg aaacgttttg caatgctgag agaataggaa cggtaaaaat
    24541 ggctgccagt gaaccgaaac ataacattga ggtacggcga ccaatcttat ccgacagcgc
    24601 gccaatgagt ggttgaataa gcatgaatac aaacaatgcg gcagtcataa tgccactcgc
    24661 cacgttggca tgcattcccg cagtatttac cagatacttc tgcatataag tggtgaaggt
    24721 atagaaacaa agggagcccg cagcggtaaa accgagaacc atgatgaatg cacggcgatt
    24781 gcgccataat cctttcagag atccagcttc ttttaaagcg cgcgtttctt gttgcgaagt
    24841 ttcatctaac tgacgacgta accacaacgc cacaacagct aacacagctc ctaacgcgaa
    24901 aggaatacgc catccccact ctctgagtgc agcgtcttcc atggtgtgtt gtaaaaccac
    24961 gacaaccagt agggctagca gttgtccgcc gatcaacgtc acatactgaa atgatgcgta
    25021 aaaacctttg cgcccttcaa cggcaacttc actcatatag gtggcgctgg tgccatattc
    25081 tccgccaacg gataatccct gaaataaacg cgcgagaagc aataatgccg gagcccacgt
    25141 accgattgtt tcataaccag gcaaacaggc gattaccagt gagccgaaac acatcataca
    25201 caccgatagc agcatcgatt ttttgcgacc atgtttatcg gctatgcggc caaatagcca
    25261 accgcctatt gggcgcatga ggaatcccgc agcaaaaaca cctgctgttt gtagtagttg
    25321 agtcgtcgtg ttcccggaag ggaagaagat gtgggcaaag tagagtgaac agaacgagta
    25381 gacatagaaa tcgaaccact cgaccagatt acctgaagag gcccccacaa tcgcccaaat
    25441 gcggcgacgt gtatcactac ttgtcagttt gctgtctgcc gttacagtac tttcagccat
    25501 gccattatgt ctcctgccgt aatccgatgc ttttgtcggt cgcttttgtt tatttctttt
    25561 ggtaaagaaa atgttataca tttgttgcgt gtcattatgc aaccttaacc atgaatttag
    25621 ttagcaggaa aatggttatt gaggagctta aggataaatt tctggtgagg agaacacgta
    25681 tggaggtggg caagttgggg aagccgtatt cgctgctgaa tctggcatat gtgggagtat
    25741 aagacgcgca gcgtcgcatc aggcattttt ttcaccaacg caaaaaggcc atccgtcagg
    25801 atggcctttc gctt
//
LOCUS       DAZFKF010000071        24544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFKF010000071 DAZFKF010000000
VERSION     DAZFKF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.071"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..297
                     /locus_tag="ACHKA1_003402"
     CDS             <1..297
                     /locus_tag="ACHKA1_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO5681009.1"
                     /translation="GRPDEGIPAVCFKLKEGEDPGYTLYDLSERLRLRGWQVPAFTLG
                     GEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            453..1988
                     /gene="gadC"
                     /locus_tag="ACHKA1_003403"
     CDS             453..1988
                     /gene="gadC"
                     /locus_tag="ACHKA1_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO5681010.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2119..3438
                     /locus_tag="ACHKA1_003404"
     CDS             2119..3438
                     /locus_tag="ACHKA1_003404"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO5681011.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3810..5192
                     /gene="dosC"
                     /locus_tag="ACHKA1_003405"
     CDS             3810..5192
                     /gene="dosC"
                     /locus_tag="ACHKA1_003405"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO5681012.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYRGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5217..7616
                     /gene="dosP"
                     /locus_tag="ACHKA1_003406"
                     /gene_synonym="pdeO"
     CDS             5217..7616
                     /gene="dosP"
                     /locus_tag="ACHKA1_003406"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO5681013.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7874..8455
                     /gene="ddpX"
                     /locus_tag="ACHKA1_003407"
     CDS             7874..8455
                     /gene="ddpX"
                     /locus_tag="ACHKA1_003407"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO5681014.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            8469..10019
                     /locus_tag="ACHKA1_003408"
     CDS             8469..10019
                     /locus_tag="ACHKA1_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5681015.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASGWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTNKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRNYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10021..11043
                     /locus_tag="ACHKA1_003409"
     CDS             10021..11043
                     /locus_tag="ACHKA1_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5681016.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGDE"
     gene            11040..11936
                     /gene="ddpC"
                     /locus_tag="ACHKA1_003410"
     CDS             11040..11936
                     /gene="ddpC"
                     /locus_tag="ACHKA1_003410"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO5681017.1"
                     /translation="MMLSEETSAVRPAKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCVFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11933..12919
                     /locus_tag="ACHKA1_003411"
     CDS             11933..12919
                     /locus_tag="ACHKA1_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5681018.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            12912..13838
                     /locus_tag="ACHKA1_003412"
     CDS             12912..13838
                     /locus_tag="ACHKA1_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO5681019.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQVNRGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWTLRKTELPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLAIRENGRELRCWRAL"
     gene            complement(13894..14325)
                     /gene="osmC"
                     /locus_tag="ACHKA1_003413"
     CDS             complement(13894..14325)
                     /gene="osmC"
                     /locus_tag="ACHKA1_003413"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO5681020.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14671..14886
                     /gene="bdm"
                     /locus_tag="ACHKA1_003414"
     CDS             14671..14886
                     /gene="bdm"
                     /locus_tag="ACHKA1_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO5681021.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            14988..15125
                     /gene="sra"
                     /locus_tag="ACHKA1_003415"
     CDS             14988..15125
                     /gene="sra"
                     /locus_tag="ACHKA1_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO5681022.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15282..16979
                     /gene="maeA"
                     /locus_tag="ACHKA1_003416"
     CDS             15282..16979
                     /gene="maeA"
                     /locus_tag="ACHKA1_003416"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO5681023.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17113..18123
                     /gene="adhP"
                     /locus_tag="ACHKA1_003417"
     CDS             17113..18123
                     /gene="adhP"
                     /locus_tag="ACHKA1_003417"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO5681024.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDV
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            18270..18554
                     /locus_tag="ACHKA1_003418"
     CDS             18270..18554
                     /locus_tag="ACHKA1_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO5681025.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(18782..19435)
                     /gene="fdnI"
                     /locus_tag="ACHKA1_003419"
     CDS             complement(18782..19435)
                     /gene="fdnI"
                     /locus_tag="ACHKA1_003419"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO5681026.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19428..20312)
                     /gene="fdnH"
                     /locus_tag="ACHKA1_003420"
     CDS             complement(19428..20312)
                     /gene="fdnH"
                     /locus_tag="ACHKA1_003420"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO5681027.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20325..23372)
                     /gene="fdnG"
                     /locus_tag="ACHKA1_003421"
     CDS             complement(20325..23372)
                     /gene="fdnG"
                     /locus_tag="ACHKA1_003421"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(22785..22787),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO5681028.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23604..24485
                     /gene="yddG"
                     /locus_tag="ACHKA1_003422"
     CDS             23604..24485
                     /gene="yddG"
                     /locus_tag="ACHKA1_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO5681029.1"
                     /translation="MTRQKATLIGLIAIILWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSMPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         5999 a         6235 c         6220 g         6090 t
ORIGIN      
        1 ggtcgcccgg acgaaggcat cccggcggtt tgcttcaaac tgaaagaggg tgaagatccg
       61 ggatacaccc tgtacgacct ctctgaacgt ctgcgtctgc gcggctggca ggttccggcc
      121 ttcactctcg gcggtgaagc caccgacatc gtggtgatgc gcattatgtg tcgtcgcggc
      181 ttcgaaatgg actttgctga actgttgctg gaagactaca aagcctccct gaaatatctc
      241 agtgatcacc cgaaactgca gggtattgcc caacagaaca gctttaaaca tacctgataa
      301 cgtttaacgg taacggtgtc ccgaaacgaa cccgtttcgg gacaatttcc aaagtctgtt
      361 cactggcatt agcaacggaa aatattgttc tgaatacgct tcagaacaaa acaggtgcgg
      421 ttccgacagg aataccgttt tagggggata atatggctac atcagtacag acaggtaaag
      481 ctaagcagct cacattactc ggcttttttg ccataacggc atcgatggta atggctgttt
      541 atgaataccc taccttcgca acatcgggct tttcattagt cttcttcctg ctattaggcg
      601 ggattttatg gtttattccc gtgggacttt gtgctgcgga aatggccacc gtcgacggct
      661 gggaagaagg tggtgtcttc gcctgggtat caaatactct ggggccgaga tggggatttg
      721 cagcgatctc atttggctat ctgcaaatcg ccattggttt tattccgatg ctctatttcg
      781 tgttaggggc actctcctac attctgaaat ggccagcgct gaatgaagac cccattacca
      841 aaactattgc agcactcatc attctttggg cgctggcatt aacgcagttt ggtggcacga
      901 aatacacggc gcgaattgct aaagttggct tcttcgccgg tatcctgtta cctgcattta
      961 ttttgattgc attagcggct atttatctgc actccggtgc ccccgttgct atcgaaatgg
     1021 attcgaagac cttcttccct gacttctcta aagtgggcac cctggttgta tttgttgcct
     1081 tcattttgag ttatatgggc gtagaagctt ccgcaaccca cgtcaatgaa atgagtaacc
     1141 cagggcgcga ctatccactg gctatgttac tgctgatggt ggcggcaatc tgcttaagct
     1201 ctgttggcgg tttgtctatt gcgatggtca ttccgggtaa tgaaatcaac ctctccgcag
     1261 gggtaatgca aacctttacc gttctgatgt cccatgtggc accggaaatt gagtggacgg
     1321 ttcgcgtgat ctccgcactg ctgttgctgg gtgttctggc ggaaatcgcc tcctggattg
     1381 ttggtccttc tcgcgggatg tatgtcacag cgcagaaaaa cctgctgcca gcggcattcg
     1441 ctaaaatgaa caaaaatggc gtaccggtaa cgctggtcat ttcacagctg gtgattactt
     1501 ctatcgcgtt gatcatcctc accaataccg gtggcggtaa caacatgtcc ttcctgatcg
     1561 cactggcgct gacggtggtg atttatctgt gtgcttattt catgctgttt attggctaca
     1621 ttgtgttggt tcttaaacat cctgacttaa aacgcacatt taatatccct ggtggtaaag
     1681 gtgtgaaact ggtcgtggct attgtcggtc tgctgacttc aattatggcg tttattgttt
     1741 ccttcctgcc gccggataat atccagggtg actctaccga tatgtatgtt gaattactgg
     1801 tcgttagttt cctggtggta cttgccctgc cctttattct ctatgctgtt catgatcgta
     1861 aaggcaaagc gaataccggc gtcactctgg agccaatcaa cagtcagaac gcaccaaaag
     1921 gtcacttctt cctgcacccg cgtgcacgtt caccacacta tattgtgatg aatgacaaga
     1981 aacactaacc atgtctgaat ggttataaga caagggagcg ataattcatc gctccctttt
     2041 tcgtgcttgc tgtcgtattg acttaaccgg gataagtacg agaatgagcg cacatctgtt
     2101 taccggaaac cagcacatat ggatatctgc tcccgaaaca agaaattaac gattagaaga
     2161 ccagcgatac tggttgcact ggcactttta ctgtgtagtt gtaaaagcac gcctccagag
     2221 tccatggtga caccaccagc gggttcaaag ccaccagcca cgacgcaaca atcgtcacaa
     2281 ccgatgcgtg gcatctggct ggccacggtg tctcgcctcg actggccacc ggtttcctcg
     2341 gttaacatta gtaaccccac cagccgggcc cgtgtacaac aacaggcgat gatcgacaaa
     2401 ttggatcatt tgcaacgtct cggtataaac acggtctttt tccaggtcaa gccggacggt
     2461 accgccctgt ggccttcaaa aattttgcca tggtccgatc ttatgaccgg taagattggt
     2521 gaaaatccgg gttacgatcc gctgcaattt atgctcgacg aagcccacaa gcgtgggatg
     2581 aaagtacacg cctggtttaa cccctatcgc gtatcggtta atacgaagcc cggtactatc
     2641 agggaactga atagcactct gtctcaacaa ccggcgagcg tctatgtgca acaccgtgac
     2701 tggatcagaa cgtctggcga tcgctttgtc ctcgacccgg gcatccctga ggttcaggac
     2761 tggatcacat caatagtcgc agaagtggtt tcccgctatc cggtagatgg cgtgcagttt
     2821 gacgactatt tctatactga gtcaccgggt tcacggctaa atgataacga aacgtaccgc
     2881 aaatacggag gcgcatttgc gtcaaaagca gactggcggc gcaacaatac tcagcagtta
     2941 attgcaaaag tatcgcacac cattaaaagc attaagccgg gagtcgaatt tggtgttagc
     3001 ccggcaggcg tgtggcgtaa ccgatcacac gatccgctcg gttccgatac ccgaggcgcg
     3061 gcagcctatg acgaatccta cgctgacacc cgtcgatggg tggaacaagg attgctggat
     3121 tacattgctc cccaaattta ctggccgttc tcacggagtg ccgcgcgtta tgacgtgttg
     3181 gcaaaatggt gggcggatgt cgttaaaccg accaggaccc gcctgtatat cggtatcgcc
     3241 ttctataaag tgggtgaacc ttcaaagata gagccagact ggatgattaa cggcggcgta
     3301 ccggaactga aaaagcagct cgatcttaac gatgcggtac cagaaattag cggcaccatc
     3361 ttgttccgtg aggactatct gaataaaccg cagactcaac aagcggtcag ctatctgcaa
     3421 agtcgttggg gcagttaaac tcccattctt actttgtaac aagccggtac attacccacc
     3481 ggcttgttac cctcttgaac tatcccacta ctaatcatgc ttactaaagt caaattaacc
     3541 atgcaattaa ccacacttag ttaaacagcc ataaatcccc taatacaaat gtatgaatat
     3601 gaaaaaacat acgatattcg ctgtcaccga aaaataatta cgatgggcta tactcttctg
     3661 aagaacagta gcgtagattg aaactgtcct aagtcgctta tttcttttca gtatatcttc
     3721 atatttcagg agaatatggt gcagttagtc gatctcgcac gttgcgtttt gattccgtct
     3781 gatttcccag ccttataagg gtgtgaatta tggagatgta ttttaaaaga atgaaagatg
     3841 agtggaccgg acttgtcgaa caggcagatc cgcccattcg agctaaagcc gcggaaattg
     3901 ccgttgcgca tgctcattat ctgagtattg agttttatcg aattgtccgc atcgacccgc
     3961 atgccgaaga attcttgagt aatgaacaag ttgagcggca gttgaaaagt gcgatggaac
     4021 gctggattat taacgtgctt tctgcccagg ttgacgatgt cgaaaggcta atacaaatcc
     4081 agcataccgt cgcggaagtg catgcccgca taggaattcc ggtagaaatt gtcgagatgg
     4141 ggtttcgggt gctgaaaaag atcctctatc cggtaatctt ctcttcggat tattctgccg
     4201 cagaaaaact tcaggtctac catttctcga ttaacagtat tgatatcgct atggaagtga
     4261 tgacccgcgc gtttaccttt agtgacagta gtgcctcaaa agaagatgaa aactatcgta
     4321 tcttctcgtt actggaaaac gccgaagaag aaaaagaacg gcaaatagcc tcaatacttt
     4381 catgggaaat agatatcatc tataaaatcc tgctggattc tgatttaggc agtagtttgc
     4441 ctttaagcca ggctgatttt ggcctgtggt ttaaccataa aggtcgacat tatttcagcg
     4501 gcattgctga agtgggccat atctctcgtc tgattcagga tttcgacggt attttcaatc
     4561 aaaccatgcg taacaccagg aatttgaata acagaagtct gcgggtgaaa tttttattac
     4621 agataagaaa taccgtatcg caaattatta ccttgctgcg tgaattgttt gaagaagtat
     4681 cgcgccacga agtcggtatg gatgtactga cgaaattact taaccgccgt ttcctgccga
     4741 ctatcttcaa acgcgaaatt gcccatgcca accggaccgg tacaccgctg tcagtgctga
     4801 ttattgacgt tgataaattc aaagagatca acgatacgtg gggccataac actggcgatg
     4861 aaattctgcg taaagtctct caggcctttt atgacaacgt ccgcagtagt gattatgttt
     4921 tccgctacag gggcgatgaa tttatcattg ttttgactga agcttctgaa aacgaaacgt
     4981 tacgtaccgc agaacgtatt cgcagtcggg tggagaaaac caaactgaaa gccgcaaacg
     5041 gcgaagatat tgccctctca ctttccatcg gtgccgccat gtttaatggt catcctgact
     5101 atgagcgcct cattcaaata gccgatgaag ctctgtatat cgccaaaaga cgaggtagaa
     5161 accgtgttga actctggaaa gccagtcttt agatgcgcca ggatgcagag gtaatcatga
     5221 agctaaccga tgcggataat gccgccgatg gcattttttt ccccgccctt gagcaaaata
     5281 tgatgggtgc ggtgttaatt aacgaaaatg atgaagtgat gtttttcaac cccgccgcag
     5341 agaagctctg ggggtacaaa cgtgaagaag tcattggcaa taacattgat atgctgattc
     5401 cgcgggattt gcgtcctgcg catcctgaat acattcgtca taaccgtgaa ggcggtaaag
     5461 cgcgcgtcga ggggatgagt cgggagctgc agctggagaa aaaagacggc agtaaaatct
     5521 ggacccgttt tgcgctatca aaagtgagcg ccgaggggaa agtttattac ctggcgctgg
     5581 tacgggatgc cagcgtagaa atggcgcaaa aagaacagac caggcaattg attattgccg
     5641 ttgaccatct cgaccgaccg gtgattgtcc tcgatccgga acgccatatt gtgcagtgca
     5701 atcgcgcatt taccgaaatg tttggttact gcattagcga agccagcggt atgcagcccg
     5761 atacactcct gaacattcct gaattccctg ccgataaccg cattcgttta caacagttgc
     5821 tatggaaaac cgcccgcgat caggacgaat ttctgctgtt gacgcgcacc ggtgaaaaaa
     5881 tctggattaa agcttctatc agcccggttt atgacgtgct cgcgcatctg cagaacctgg
     5941 taatgacctt ctcggatatc accgaagaac ggcaaatccg ccagcttgaa ggcaatattc
     6001 tcgccgccat gtgcagcagc ccgccatttc atgaaatggg ggaaatcatt tgtcgtaata
     6061 tcgaatctgt tctcaacgaa tcgcatgttt cgctgttcgc actgcgcaac gggatgccga
     6121 tacactgggc gtcatcttcc cacggtgcag aagttcaaaa tgcgcaaagc tggtcagcga
     6181 ccattcgtca gcgtgatggc gcgcctgcgg ggatcctgca aattaaaacc tcgtcaggag
     6241 cagaaaccag cgcctttatc gaacgcgtgg cagatatcag ccagcatatg gccgcgctgg
     6301 cgctggaaca ggaaaaaagc cgtcagcata ttgaacaact catccaattt gatccgatga
     6361 ccggtctgcc aaatcgcaat aacctgcaca attacctcga tgacctggtc gacaaagccg
     6421 tctctccggt ggtgtatctc atcggtgttg accatattca ggatgtgatt gatagccttg
     6481 gctatgcgtg ggccgatcag gcattgctgg aagtggtcaa tcgctttcgt gaaaaactca
     6541 aaccggatca gtatctctgt cgtatcgaag gtacgcagtt tgtcctcgtg agcctggaaa
     6601 acgacgtcag taacattacc caaatcgccg atgagctacg gaatgtggtc agcaagccga
     6661 taatgattga cgataaaccc ttcccgctta ccttgagtat tggcatcagc tacgacgtgg
     6721 gtaaaaaccg cgattacctg ctctccactg ctcacaatgc aatggattat attcgcaaga
     6781 atggcggtaa cggctggcag ttcttcagcc cggcgatgaa cgaaatggta aaagagcgtt
     6841 tggttttagg cgcagcgctg aaagaggcga ttagcaataa ccaactgaaa ctggtttacc
     6901 agccgcaaat cttcgcagaa acgggtgaac tgtacggcat cgaagccctt gctcgctggc
     6961 acgatcccca gcatggtcat gtgccccctt cacggtttat tcctctcgca gaagagattg
     7021 gtgaaatcga aaatattggg cgctgggtca tcgcggaagc ttgccgtcag ttagcagaat
     7081 ggcgtagcca gaatattcat atcccggcgt tatccgtgaa cttgtcggcg ctgcactttc
     7141 gcagtaatca actgcctaat caggtgtctg atgcaatgca agcctggggt attgacggcc
     7201 accagctgac ggtggaaatc acggaaagca tgatgatgga acacgatacc gaaatcttta
     7261 agcgcattca gatcctgcgt gatatgggcg tgggtttatc ggtagatgat tttggcacgg
     7321 gcttttccgg attatcccgc ttagtcagtc ttccggtaac ggaaatcaaa attgacaaaa
     7381 gttttgtcga tcgttgtctg acggaaaaac gcatccttgc cttacttgaa gccattacca
     7441 gcattgggca aagcctcaat ttaaccgtcg tggcggaagg cgtcgaaacc aaagagcaat
     7501 ttgagatgct acgtaagatc cactgtcgag ttattcaggg atatttcttt tcccgccccc
     7561 tacccgccga agaaattcca ggctggatga gcagcgtgtt accgctgaaa atctgacaaa
     7621 ttcctctcgc ccgcactcgc gggttttcat ttaacgtgac actgtcactt aaatactgtg
     7681 atttcagcta cgattccggg agattccttc cttaacttcc cctcttgcac caaaactgca
     7741 ctacgctgca caaatgttga tcaaaagtta aaaaatataa ctttaagcca gcattctggc
     7801 gcttatcccg gcatggcatg agatctgcat aagcggaaag cgcagcaatt tttgtcttat
     7861 acaggaaccg tttatgtcgg ataccaccga actggttgat ttagccgtga tcttccctga
     7921 tctggagatc gaattgaaat acgcctgcgc tgataacatc acaggtaaag ctatttatca
     7981 gcaagcgcgt tgtctgttac acaaggatgc gattaccgcg ctggcgaaaa gtatcagtat
     8041 cgcccagctc tcaggcttac aactggtgat ttacgatgcg taccgcccac aacaagcaca
     8101 ggcgatgttg tggcaagcct gcccagaccc gcaatatgtt gttgatgtga cggtcggttc
     8161 taatcacagc cgtggcacgg cgatcgacct gacgcttcgt gatgagcacg ggaacatcct
     8221 cgatatgggc gcagggttcg atgaaatgca cgagcgctcc catgcctatc acccttccgt
     8281 cccgcccgcc gctcagcgca atcgcctgtt gctgaatgcg ataatgactg gtggcggttt
     8341 tgtcggtatc tccagcgaat ggtggcactt cgaattaccc caggcagcga gttacccact
     8401 gctcgctgat caattcacct gttttatatc ccctggcaca cagcacgtca gttaatcagg
     8461 aaactgtcat gaaaagatcg atatcgtttc gtcctacatt gctcgcgctc gtccttgcca
     8521 caactttccc ggttgcgcac gccgccgtac cgaaagatat gctggtgatt ggtaaggccg
     8581 ccgatccaca aaccctcgac ccggcggtaa caatagataa taacgactgg acggtgacct
     8641 acccgtctta tcagcggctg gttcagtaca aaacggacgg tgataaaggc tcaaccgacg
     8701 ttgaaggcga tctggcgagt ggctggaaag cgtctgacga tcaaaaagag tggacgttca
     8761 ccctgaagga caatgctaaa tttgccgatg gcacacccgt cactgccgaa gcagtaaaac
     8821 tctcttttga gcggttacta aaaatcggcc aggggccagc agaagcattt cccaaagatt
     8881 taaagattga tgctcccgac gaacatacgg tgaagtttac ccttagccag ccatttgcac
     8941 cgttcctcta cacgctggcg aatgacggtg catccattat caatccggcg gtgttaaagg
     9001 aacatgcggc ggatgatgcc cgcggcttcc tcgcgcaaaa taccgccggt tccggaccat
     9061 ttatgctgaa aagctggcaa aaaggtcagc aattagttct ggtgccaaat ccgcattacc
     9121 ctggcaataa accgaacttt aagcgagtat cggtaaaaat tattggtgaa agtgcctccc
     9181 gtcgcctgca gctctcccgt ggcgacattg acattgccga tgcgctgccg gtggatcaac
     9241 tcaacgccct gaagcaggaa aataaagtca atgtggcaga gtatccgtca ctgcgcgtca
     9301 cctatctgta tctgaataac agcaaagcgc cacttaatca ggcggatctg cggcgggcca
     9361 tttcctggtc taccgattat cagggcatgg ttaacggcat tctgagtggt aacggaaaac
     9421 agatgcgcgg cccgattccg gaaggcatgt ggggctacga tgcgacggca atgcaataca
     9481 accatgacga aacgaaagcc aaagctgaat gggataaagt gacgaacaaa cccaccagcc
     9541 tgacgtttct ctattctgat aatgatccga actgggagcc tattgctctg gcgacacaat
     9601 ccagtctcaa caagctgggc atcaatgtga agctggaaaa gctggcgaac gccaccatgc
     9661 gcgacagagt gggtaaaggt gattacgaca ttgcgattgg caactggagt ccggattttg
     9721 ccgacccgta tatgtttatg aattactggt ttgagtcaga caaaaaaggt ctgccgggta
     9781 accgctcgtt ctatgaaaac agtgaggtcg ataagttact gcgcaatgcg ctcgcgacca
     9841 ccgaccagac gcagcgtacc cggaactacc agcaggcaca gaaaatcgtc attgatgacg
     9901 ctgcttatgt gtacctgttc cagaaaaact accaactggc gatgaacaaa gaggtgaaag
     9961 gctttgtgtt caatcccatg ctggaacagg tcttcaatat caataccatg agtaaataac
    10021 gtgaccttct ggagtatttt acgccaacgc tgctgggggc tggtgctcgt ggtggcgggc
    10081 gtctgcgtga ttacgtttat tatctcgcat ctgatccccg gcgatccggc gcggttactg
    10141 gcgggtgacc gcgccagcga tgctatcgtg gaaaatattc gccagcaact gggactggac
    10201 cagccactgt atgtacagtt ttaccgctac gtcagcgacc tgtttcatgg tgacctggga
    10261 acatccattc gtaccgggcg tccggtgctg gaagagttgc gtatattttt cccggcgacg
    10321 ctggagctgg ctttttgtgc cctgctgctg gcactcttga ttggcatccc gctgggcata
    10381 ctctctgcag tctggcgaaa tcgctggctg gatcatctgg tgcgaataat ggccattacc
    10441 ggaatctcca cacctgcgtt ctggcttgga ctgggcgtca ttgtgctgtt ttatggtcat
    10501 ctgcaaattc ttcccggcgg cggaaggctt gatgactggc tggatccacc aacgcacgtt
    10561 accggctttt atctgctcga tgcgctgctt gaaggcaacg gtgaagtatt cttcaatgcg
    10621 ttgcaacatc tcatcttacc ggcattaacg ctggcgttcg ttcacctggg aattgtcgct
    10681 cgccagatcc gctcagcgat gctggaacaa ttgagtgaag actacattcg taccgcccgg
    10741 gccagcggct tgcccggctg gtatatcgtt ttatgttatg cgctacccaa tgcgttgatc
    10801 ccatcgatta ccgtattggg tctggcgctg ggcgatttgt tgtatggcgc agtgctcacc
    10861 gaaaccgttt ttgcctggcc cggaatgggg gcatgggtag tgacatcaat acaggcgctc
    10921 gacttccctg cggtgatggg atttgccgtc gtggtttcgt ttgcttatgt gctggtcaac
    10981 ctggtagtgg atttgctcta tttgtggatt gatccgcgta tcggacgtgg aggtgatgaa
    11041 tgatgctaag cgaggaaacg tccgccgtac gcccagcaaa acaaacgcga tttaacggtg
    11101 caaaactggt atggatgctg aaaggcagtc cgctcaccgt gaccggcgca gtcatcattg
    11161 tattaatgct attgatgatg attttttcac cgtggcttgc gacgcatgat cccaacgcca
    11221 ttgatttaac cgcccgcctt ttgccgcctt ctgcggcgca ctggtttggc accgatgaag
    11281 tgggacgcga tctgttcagc cgcgtactgg tcggcagtca gcaatcaatt ctcgccggat
    11341 tagtcgtggt tgccattgcg ggtatgattg gttcgctact cggatgtcta tccggtgtgc
    11401 ttggcggacg cgcagacgcc atcatcatgc gcatcatgga cattatgctg tcgattcctt
    11461 cgttggtact gacaatggca ctggctgccg ctctcgggcc gagtttattt aacgccatgc
    11521 tggcgattgc tattgtgcga attccctttt atgtgcgcct ggcgcggggg caagcattag
    11581 ttgtacgcca gtatacctat gttcaggcgg cgaaaacctt tggtgcgtcg cgttggcatc
    11641 tgatcagctg gcatatttta cgtaactccc tgccgccgct gatcgtgcag gcatcgctgg
    11701 atatcggtag cgcgatttta atggccgcca cgttgggatt tattggcctg ggtgctcaac
    11761 aaccgagtgc tgaatggggc gcgatggtag caaatggtcg caactatgta ctcgatcagt
    11821 ggtggtattg cgtatttccg ggggcagcga ttttgcttac cgccgtcggg tttaatctct
    11881 ttggtgatgg tattcgcgat ctgcttgacc cgaaagcagg aggaaagcag tcatgaccca
    11941 acccgttctg gacattcaac agctgcattt gagtttcccc ggttttaacg gtgatgttca
    12001 cgcgctcaac aatgtgtcct tgcagattaa ccgcggtgaa attgtcggtc tggtgggaga
    12061 atccggctca ggtaaatcag tcaccgcaat gctgattatg cgtctgctac cgacgggcag
    12121 ttattgcgta catcgggggc agatttcact gctgggagac gatgttctta acgctcggga
    12181 aaaacagctt cgtcagtggc gcggcgcacg agtggcgatg atctttcagg aaccgatgac
    12241 cgccctcaat ccgacacgtc gaataggtct tcagatgatg gacgtgatcc gccatcatca
    12301 accaataagt cgtcgggaag ccagagctaa agcgattgcc ctgctggaag agatgcaaat
    12361 cccggatgcc gtggaagtta tgtcgcgcta tccgtttgag ctttcaggtg gtatgcgcca
    12421 gcgggtaatg attgcgctgg cattctcctg cgagccgcaa ttgattattg ccgacgaacc
    12481 gactacggcg ctggacgtca cggtacagtt gcaggtactg cgtttgctta aacataaagc
    12541 ccgcgccagt ggaactgcgg tactgttcat cagccatgat atggccgtgg tgtcgcaact
    12601 gtgcgatagc gtttacgtga tgtatgccgg aagcgtaatt gaaagcggcg ggacggcaga
    12661 cgttatccat catccccggc atccgtatac cattggtttg ctgcaatgcg caccagaaca
    12721 tggagtacca cgccagccat tacccgccat tccagggacg gtaccaaacc tcacccattt
    12781 gcctgacggc tgcgcttttc gcgatcgttg ctatgcggca ggtgcacagt gtgaaaacgt
    12841 cccggcgctg acagcgtgtg gtgacaacaa ccagcgctgc gcctgttggt atccgcagca
    12901 ggaggtcata agtgtctgac acgttattaa cgttacgcga cgtccatatc aatttcccgg
    12961 cccgtaaaaa ctggcttggt aaaactacgg aacatgttca tgccattaat ggtattgatt
    13021 tacagatccg ccgtggtgaa accttaggga tcgtcggcga gtcaggctgc ggcaaaagca
    13081 ccctcgcaca gcttttaatg ggtatgctgc aaccgagcca cgggcaatac atccgttctg
    13141 gctcacaacg cattatgcag atggtgtttc aggacccgct ctcttcgctc aatccgcgct
    13201 taccggtgtg gcgcatcatc acagaaccgc tctggatagc taagcgtagt agtgaacaac
    13261 agcggcgagc gttggcagag gagctggctg tgcaggtggg tattcgtccg gagtatctcg
    13321 accgcctgcc tcatgcgttc tccggcgggc agcggcaacg catcgccatt gccagagcac
    13381 tctcttcgca gcctgacgtg attgtgcttg atgagccaac ctcggcgctg gatatctccg
    13441 tgcaggcgca gatcctcaat ttactggtaa cgctacaggt aaatcgcggg ctgacctatg
    13501 tgctgatctc acacaatgtc tcggtgattc gtcatatgag cgatcgggta gcggtgatgt
    13561 atctcgggca gattgtagaa ctgggcgacg cgcaacaggt actgacggct ccggcacatc
    13621 cgtacacccg attattgctg gattccctcc ccgccattga taaaccgctg gaggaagaat
    13681 ggacattacg taaaacggaa ctgccgggaa accgcacgtt gccgcaaggc tgttttttct
    13741 acgaacgttg ccctctggca acccacggat gtgaagtccg gcaatcatta gcaataaggg
    13801 agaacggacg tgagctccgg tgctggcggg cgctgtagag atgctaacaa cgcatcaggc
    13861 attcacgcgc ctgacgcgtc atccggcaat gctttacgat ttcaactggt aatccagtgt
    13921 aatttccgct ttcagtacct gagagaccgg gcatcctgct tttgctttct ggatgatgcc
    13981 gtcaaaggta gaggcatcaa tacccggcac cgcaacttca ctcttcagtg cgattttcgt
    14041 aatcgcaaaa ccggcatcca ctttatccag cgacacatcg gcggtggtat caatcgatgt
    14101 tggcgtgaat cccgcttccc ccagcattaa tgaaagcgcc attgagaaac atgcggcatg
    14161 cgctgcgcca atcagttctt cagggttggt tcctttttcg ccttcaaaac gcgtgttaaa
    14221 tccatacggc tgttggttca gcacgccact ctcggtggat actgttccct tcccgcgttt
    14281 gatatcgcct tcccagtgtg cctgaccttt cttatggatt gtcattgttg ctctcctgtg
    14341 ggcttaaaaa ggaaattata gactacgtga gaaacagcac gaggataagc aggatattcc
    14401 gaataaaatc cggcagaaaa tattacttct gcacttaaat taaatttcca caatagataa
    14461 ccgggaatat atagctcaag agatattgca tcaatatcat tcaactcacc acaataataa
    14521 tacctatcaa tatcatgggg ttaaaatata aaaatcatca accaggacta atcttaacaa
    14581 cgaagcggca aatatttgcc atgctgaatg tgctttataa atctgcgatc cgtagcagac
    14641 actataaata cacagacacg gagaatcact atgtttactt attatcaggc agaaaattca
    14701 acagcagaac ccgctctggt taatgccatt gagcaaggcc ttcgggcaga gcacggtgtt
    14761 gtcactgaag atgacattct catggagctg accaaatggg tggaagcttc tgataacgac
    14821 atcctcagtg atatctacca gcaaacgatc aattatgtgg tcagtggcca gcaccctacg
    14881 ctttaagatg ctatgcttga tcggcaacct aatttagggg tttagcacgt gtttcttcgc
    14941 tacggcgatg ttgtccttaa aactagcaac aggattgagg agttaaaatg aaatcgaacc
    15001 gtcaggcacg tcatattctt ggactggacc ataaaatttc taaccagcgc aaaatagtta
    15061 ccgaaggtga caaatccagc gtggtaaata acccaaccgg cagaaaacgc cccgctgaaa
    15121 agtaattcat aaccatcagt cctcaatgac gattaaacac cattgcctgc gcaatggtgt
    15181 ttttgttttt atctgcttta tacttggggc cgacgccctg gcggtaaagc aaagacgata
    15241 aaagcgtgcc agggatggat attcaaaaaa gagtgagtga catggaacca aaaacaaaaa
    15301 aacagcgttc gctttatatc ccttacgctg gccctgtact gctggaattt ccgttgttga
    15361 ataaaggcag tgccttcagc atggaagaac gccgtaactt caacctgctg gggttactgc
    15421 cggaagtggt cgaaaccatc gaagaacaag cggaacgagc atggatccag tatcagggat
    15481 tcaaaaccga aatcgacaaa cacatctacc tgcgtaacat ccaggacacc aacgaaaccc
    15541 tcttctaccg tctggtaaac aatcatcttg atgagatgat gcctgttatt tataccccaa
    15601 ccgtcggcgc agcctgtgag cgtttttctg agatctaccg ccgttcacgc ggcgtgttta
    15661 tctcttacca gaaccgccac aatatggacg atattctgca aaacgtacca aaccataata
    15721 tcaaagtgat tgtggtgact gacggtgaac gtattctggg gcttggtgac cagggcatcg
    15781 gcgggatggg cattccgatc ggtaaactgt cgctctatac cgcctgtggc ggcatcagcc
    15841 cggcgtatac ccttccggtg gtgctggatg tcggcaccaa caaccaacag ctgcttaacg
    15901 atccgctgta tatgggctgg cgtaatccgc gtatcactga cgacgaatac tatgaattcg
    15961 ttgatgaatt tatccaggct gtgaaacaac gctggccgga cgtgctgttg cagtttgaag
    16021 acttcgcaca aaaaaatgcg atgccgttac ttaaccgcta tcgcaatgaa atttgttcct
    16081 ttaacgatga cattcagggc actgcggcgg taacagtcgg caccctgatc gcagcaagcc
    16141 gcgcggcagg tggtcagtta agcgagaaaa aaatcgtctt ccttggcgca ggttcagcgg
    16201 gatgcggcat tgccgaaatg atcatcgccc agacccagcg cgaaggatta agcgaggaag
    16261 cggcgcggca gaaagtcttt atggtcgatc gctttggctt gctgaccgac aagatgccga
    16321 acctgctgcc tttccagacc aaactggtgc agaagcgcga aaacctcagt gactgggata
    16381 ccgacagcga tgtgctgtca ctgctggatg tggtgcgcaa tgtaaaacca gatattctga
    16441 ttggcgtctc aggacagacc gggctgttta cggaagagat catccgtgag atgcataaac
    16501 actgtccgcg tccgatcgtg atgccgctgt ctaacccgac gtcacgcgtg gaagccacac
    16561 cgcaggacat tatcgcctgg actgaaggta acgcgctggt cgccacgggc agtccgttta
    16621 atccagtggt atggaaagat aaaatctacc ctatcgccca gtgtaacaac gcctttattt
    16681 tcccgggcat cgggctgggt gttattgctt ccggcgcgtc acgtatcacc gatgagatgc
    16741 tgatgtcggc aagtgaaacg cttgctcagt attcgccgct ggtcctgaac ggcgaaggtc
    16801 tggtactacc ggaactgaaa gatattcaga aagtctcccg cgcaattgcg tttgcggttg
    16861 gcaaaatggc gcagcagcaa ggcgtggcgg tgaaaacgtc tgccgaagct ctgcaacagg
    16921 ccattgatga taatttctgg caagccgaat accgcgacta ccgccgtacc tccatctaag
    16981 cctgcgcccg gtagtgaagg ctaccgggca attttcctct ccctttttca gatctcatcc
    17041 atactgggta gtggcgaata aatctcattt gcctcacctg ctatgcagaa catcatccga
    17101 aaaggaggaa ctatgaaggc tgcagttgtt acgaaggatc atcatgttga cgttacggat
    17161 aaaacactgc gctcactgaa acatggcgaa gccctgctga aaatggagtg ttgtggtgta
    17221 tgtcataccg atcttcatgt taagaatggc gattttggtg acaaaaccgg cgtaattctg
    17281 ggccatgaag gcatcggtgt ggtggcagaa gtgggtccag gtgtcacctc attaaaacca
    17341 ggcgatcgtg ccagcgtggc gtggttctac gaaggatgcg gtcattgcga atactgtaac
    17401 agtggtaacg aaacgctctg ccgttcagtt aaaaatgccg gatacagcgt tgatggcggg
    17461 atggcggaag agtgcatcgt ggtcgccgat tacgcggtaa aagtgccaga tggtctggac
    17521 tcggcggcgg ccagcagcat tacctgtgcg ggggtcacca cctacaaagc cgttaaactg
    17581 tcaaaaattc gtcccggtca gtggattgct atctacggtc ttggcggtct gggtaacctc
    17641 gccctgcaat acgcgaagaa tgtctttaac gcgaaagtga tcgccattga tgtcaatgat
    17701 gagcagttaa aactggcaac cgaaatgggt gcagatttag cgattaactc acgcaccgaa
    17761 gacgtcgcca aaattgtgca ggagaaaacc ggtggcgctc acgctgcggt ggtaacagca
    17821 gttgctaaag ctgcgtttaa ctcggcagtt gatgctgtcc gtgcaggcgg tcgtgttgtg
    17881 gctgtcggtc tgccgccgga gtctatgagc ctggatatcc cacgtcttgt gctggatggt
    17941 attgaagtgg tcggttcgct ggtcggcacg cgccaggatt taactgaagc cttccagttt
    18001 gccgccgaag gtaaagtggt gccgaaagtc gccctgcgtc cgttagcgga catcaacacc
    18061 atctttaccg agatggaaga aggcaaaatc cgtggccgca tggtgattga tttccgtcac
    18121 taagaggcct ttgctgcgac tgccagtcct ggtgtcgcag catcgcacac tctccaacat
    18181 gaaatggcta aatggattat tgttgcgtag gaaggaacaa taatgatagt gttcccagtg
    18241 aaggaacact actgagagta aagacactga tgaaaatggc caatcatccc cgcccggggg
    18301 acattattca ggaatcactg gatgaactta atgtcagcct gcgcgagttt gccagagcaa
    18361 tggaaattgc gccctcaacg gcaagccgat tgctgaccgg aaaagcagct ttgacgccag
    18421 aaatggcaat taaactttcc gtggtgattg gcagttcgcc gcaaatgtgg ctgaatctgc
    18481 aaaacgcctg gagtctggca gaggctgaaa aaacggtgga tgtgtcgcga ttacgccgtc
    18541 tggttacgca ataaaaaatg gcaccttata aagcgccatt tgaaactgat gacaaatgca
    18601 acattgcctg atgcgctacg cttatcaggc ctacacgttc actgcaatct attgtatttg
    18661 aagggttttc atgccggata aggcattcac gacgcatccg ggatgaacaa agcccacgtt
    18721 gtctacaata tgaaaatggc gccttttaca gcgccatttt cagcttaaag ttcaatagag
    18781 attatatccc ttcttcactc tcttttttcg cttctgcctt ctcgatttca cgataccagc
    18841 gcggatggtg tttcttcgcc cagcgacggc tcaccttccc ttcgatcatc cctttaatcg
    18901 atcctttcac ccagaatgcc atatacatat ggatcaggat ggcgtggatc aggatgatac
    18961 ccgcagccgc gtggatcagc aggctgtagc gaacaacctg catcgggaag tactgcgcaa
    19021 agtacggacg ccagataatc accccggtca ccagcagcac gaaaatcatg ctcatgatcg
    19081 accagaacat catcttttgc ccggcgttgt acttaccgac atccgccact ttgtgctcat
    19141 tgccttttaa tacttcgaca atgttcaaca gccacggaat atctttctta tccgggatgt
    19201 tgtgatgcac aaaacgcaca aacataaaca tcagtgcgac gaaaatcgca atgccgaaga
    19261 acgggtgcaa aatgcgtccc atctgcggcg taccgaaggt ttgcgtcagc cattgcagcg
    19321 tcgggaagaa gaacgaaatc ccggacagcg ccaccaggaa gaagcaaatc accacggtcc
    19381 agtgacaggc gcgatcaata aatttggtgc gcacaatcat tttcgactta ctcatgatga
    19441 tcctcctcgt catcgtccac ttccttattc gggccaatac cgatgtaatg gaaaatcaac
    19501 cctgcaaaag tggcaataaa gccagccgct gccagcggtt tcaacgcgcc tttccacagg
    19561 cttaccgagg tgtcgatctt cggatctttc ggcagaccgt gatacagctc cggctgatcg
    19621 gcgtgatgca gcacgtacat aacgtgcgta ccaccgaccc cttccgggtt gtagacgcca
    19681 gcatgttcgt aaccacgcgc tttcagtttc gccacgcgct gttccgccag ctccagcatc
    19741 tccttcttgg tgccgaagtg gatagccccg gtcggacagg ttttcacaca agccggttcc
    19801 tggccgacgc tgacgcgatc gacgcagagc gtgcatttat atacccggtt atcctctttg
    19861 ttgaggcgcg gaatattaaa cggacacccg gcaatgcagt aaccacagcc gatgcagttt
    19921 tccgactgga aatcgacaat cccgttagcg tactgaatga ttgcaccagc agacgggcac
    19981 gccttcaggc agccgggatc ttcacagtgc atacagccgt ctttacggat cagccactcc
    20041 agcttgccgt tctgttcggt ttcgctaaag cgcatcaccg tccaggactt ggcgctcaga
    20101 tcggcggggt tatcataaac cccgacgcag tgccccactt catcacggat gtcgttccac
    20161 tccgaacacg ccacctgaca ggctttacag ccgatacagg tggaaacgtc gataagtttc
    20221 gcgacttctg ctttgtaatc acgcacctga gaaggcggcg tgatggagtt agttgcggac
    20281 cttttgataa tgtcctgcgt ttccatagcc atctgttcgc ccccttacgc cttctcgatg
    20341 ttgactaaga acgctttata ttccggcgtt tgcgagtttg catcaccgac attcggcgtc
    20401 agagtgttag cgatataacc tttacgcgcg acaccctcaa agccccagtg gattggaata
    20461 cccaccgttt caacctgctg accgtttaca ttcagcggtt tcagacggcg cgttaccaca
    20521 gccaccgcgc ggataaagcc acgcttgctt gagacagtga cacgatcgcc attattaatg
    20581 cctttcgccg ccgccagcgt ttcgctgatt tccacaaact gttccggctg agcaattgcg
    20641 ttgagcaacg cgtgcttggt ccaggtgtgg aagtgctcgg tcagacgata ggtcgtaccc
    20701 acatacggga actgctcttt tttacccatc cgcagcgcgt cttgttcata cagacgaacg
    20761 accgggttag acaccacgtt cggatgcagc gggttagtgc ccagcggcgt ttcaatcggc
    20821 tcgtagtgtt ccgggaacgg accttccgcc attttgttga tggcaaacag gcgtcccatc
    20881 ccttccggct gcataataaa cggcccggtt ggcgtacccg gtgcggcatt gccgaagtca
    20941 ggaatatcgt tacccgtcca cttgctgccg ttccactgga tcagcatccg tttcggatcc
    21001 cacggtttac cgttgatatc cgccgaagca cggttgtaca gcacgcgacg gttgagcggc
    21061 cacgcccagg cccatcccag cgtattcccc agaccggacg ggtcggagtt atcgcggtta
    21121 gccatctggt tgccctgctc tgtccagcta ccggtgtaga tccagcaaga agatgcggtt
    21181 gtaccgtcat cacgcagatg cgcaaagcta ctcagcaact gacctttctt cgcaatcagc
    21241 acgccattag catcatagag atcttccagc gcatagccgt tgttctcttt tgccacttcg
    21301 tcagattgcg gttcgtgcgg ctgcttgtag ttccagctca tcttcatcag cggttctacg
    21361 cctttaccac cttcggcctg gtacagctcg cgcagatgat ggtaaatacc cgccagaatt
    21421 tcgccgtcgt tacgcgcttc gcccggcgcg tcctgacctt tccagtgcca ctgcaaccag
    21481 cgaccggagt tagcgataga accatcttct tcagcaaagc aggtcgaagg cagacggaat
    21541 acttcagtct gaatagacgc cggatcgaca tcgttcgact cgccgtggtt ctgccagaag
    21601 gtagaggttt cagtcaccag cggatcgata accaccatgt acttcagctt gctcaggcag
    21661 ctcaccactt tgtttttgtc cgggaaggac gcaaccgggt taaagccctg gcagaaataa
    21721 ccggtgactt tgccttcgtc catcatgttg aaatacttga tgacgtcgta ggtctggtcc
    21781 cacttcggca gccagtcata gccccagttg ttctctttct gcgcggcatc gccatagaaa
    21841 gatttcatca ggctaacgaa gaacttcgga tagttgctcc agtagttcac ctgatcagcc
    21901 agcgtcgctt tcggcgtgtt cgcttccaga tacgactgca aatcaacctg tttttctgac
    21961 ggcagcgtca gataacctgg caggctggta gagagcagac ctaagtcagt cagaccctga
    22021 atgttggagt gaccacgcaa tgcattcacg ccgccaccgg ccatacccat gttaccgagc
    22081 agcaactgga tcatcgccat agtacggatg ttctgcgcac ccacggtgtg ctgcgtccag
    22141 cccagcgcgt acaggaaggt ggttgtgcga tccggtgcgc tggtggaggc cagcacttca
    22201 cacactttca ggaagtcagc ttttggcgta ccacagatgt tttcaacaac gtccggcgtg
    22261 tagcgggaaa cgtgcgcttt cagcaggttc cacacacagc gcggatgagt cagtgtttca
    22321 tcgcgtttcg catagccgtt ttcatcgaac tgatagttcc aggacgattt atcgtattgc
    22381 cgtttttcag cgtcgtagcc gctgaacaga ccgtcttcga aagcaaaatc atcacgcacc
    22441 agcaagctgg cgttggtgta atgcttaacg tattcggcgt tgattttgtt gttttcgatc
    22501 aggtagcgca aaacgccaga caggaacgta atgtccgtac cggaacgaat aggtgcgtaa
    22561 atatccgcta cagaagcggt acgcgtaaaa cggggatcga caacgatcaa ggttgcatcg
    22621 ttgttgtttt tcgcttccat cgcccagcgg aaaccgacgg gatgcgcttc agcagcgtta
    22681 ccgcccatta ccattacgac gttagcgttt ttgatatcca cccagtggtt ggtcatcgca
    22741 ccgcgaccaa atgttggagc aagacttgct accgttggtc cgtgtcagac gcgcgcctgg
    22801 ttgtctaccg ccagcatccc gagggagcgg gcaaatttct gggtcagcat cccggtttcg
    22861 ttgctggcac cggaagcaca cagcataccg gtagaaagcc aacggtttac cgttacgccc
    22921 tgctcgttct tttcaataaa gttagcgtca cggtcagctt tcatcagctt cgcaatacgg
    22981 gagaatgctt cttcccagct aatgcgctgc catttgtcag aacctggcgc acgatattcc
    23041 gggtagcgca gacggttttc gctgttgacg taatccagca aaccggcccc tttcgggcac
    23101 agcgcaccac ggcttaccgg atgatccgga tcaccttcaa tgtgataaat cgcttctctg
    23161 gcgtttttcg cgccatcacc caggctatac atcaatagcc cgcaacctac ggaacagtat
    23221 gtgcaggtgt tacggatctc tttagcgcgt aataatttgt agtttcgcgc ctgagccagt
    23281 gcttgcttcg gggcaaagcc caatgccgct actgttgttc cagccatacc gcccgcgcag
    23341 attttaaaaa attgtctgcg actgacgtcc attgctttcc tcttttttca ggggtattac
    23401 tgcgcgagga aactaacagt aaaattctga atctttttgc gcgaaatcaa aaagaacacg
    23461 tgtttgccac aaaataatcc gcctgcgcaa attgacctac ctcaatagcg gtagaaaaac
    23521 gcaccactgc ctgacaggcc agttaaaaaa atgctataaa attcagctta atttttaacg
    23581 gcaaaagaga caaaacagcg agcatgacac gacaaaaagc aacgctcata gggctgatag
    23641 cgatcatcct gtggagcacg atggtaggat tgattcgcgg tgtcagtgag gggctcggcc
    23701 cggtcggcgg cgcagctgct atctattcat taagcgggct gctgttaatc ttcacggttg
    23761 gatttccgcg tattcggcaa atcccgaaag gctatttact cgccgggagt ctgttattcg
    23821 tcagctatga aatctgtctg gcgctttcct tagggtatgc ggcgacccgt catcaggcga
    23881 ttgaagtggg tatggtgaac tatctgtggc ccagcctgac aattctcttt gccattctgt
    23941 ttaatggtca gaaaaccaac tggctgattg tacctggatt attattagcc ctcgtcggcg
    24001 tctgctgggt gttaggcggt gacaatgggt tacattatga tgaaatcatc aataatatca
    24061 ccaccagccc attgagttat ttcctggcgt tcattggtgc gtttatctgg gcagcctact
    24121 gcacagtaac gaataaatac gcacgcggat ttaatggaat taccgttttt gtcctgctaa
    24181 cgggagcaag tctgtgggtt tactattttc tcacgccaca accagaaatg gtatttagca
    24241 tgcccgtcat gattaaactc atctctgcgg catttacctt aggatttgct tatgctgcat
    24301 ggaatgtcgg tatactgcat ggcaacgtca ccataatggc ggtaggttcg tattttacgc
    24361 ccgtactttc ctcagcgctt gcagccgtgc tgctcagtgc cccgctgtcg ttctcgttct
    24421 ggcaaggcgc gctgatggtc tgcggcggtt ccctgctctg ctggctggcg acacgtcgtg
    24481 gctaaaataa ttgccgggtc tacggcccgg caattaatca gataaaccct gacaatatta
    24541 aaaa
//
LOCUS       DAZFKF010000072        24515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFKF010000072 DAZFKF010000000
VERSION     DAZFKF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.072"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            49..1236
                     /gene="ompC"
                     /locus_tag="ACHKA1_003423"
     CDS             49..1236
                     /gene="ompC"
                     /locus_tag="ACHKA1_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO5681030.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEVYNKDGNKLDLYGKVAGLHYF
                     SDDAGSDGDMSYARIGFKGETQIADQFTGYGQWEFNIGANGPESDKGNTATRLAFAGF
                     GFGQNGTFDYGRNYGVVYDVEAWTDMLPEFGGDTYAGADNFMNGRANSVATYRNNGFF
                     GQVDGLNFALQYQGNNEKSGLFDQEGSGNGNGRKLAKENGDGYGMSTSYDFDFGLSLG
                     AAYSNSDRTDNQVHKGTHNTRYGDRFDATAGGETAEAWTVGAKYDANNVYLAAMYAET
                     RNMTGYGDADAIANKTQNFEVVAQYQFDFGLRPSIAYLQSKGKDLGGVNSDNFDSQGN
                     HHYTNKDLVKYVDIGMTYYFNKNMSTYVDYKINLLDNDDDFYKENGIATDDIVAVGLV
                     YQF"
     gene            1829..2680
                     /gene="hchA"
                     /locus_tag="ACHKA1_003424"
     CDS             1829..2680
                     /gene="hchA"
                     /locus_tag="ACHKA1_003424"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO5681031.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(2788..4146)
                     /gene="hprS"
                     /locus_tag="ACHKA1_003425"
     CDS             complement(2788..4146)
                     /gene="hprS"
                     /locus_tag="ACHKA1_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO5681032.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEVCF
                     NVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(4146..4817)
                     /gene="hprR"
                     /locus_tag="ACHKA1_003426"
     CDS             complement(4146..4817)
                     /gene="hprR"
                     /locus_tag="ACHKA1_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO5681033.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4950..5363
                     /gene="hiuH"
                     /locus_tag="ACHKA1_003427"
     CDS             4950..5363
                     /gene="hiuH"
                     /locus_tag="ACHKA1_003427"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO5681034.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5471..6475
                     /gene="msrP"
                     /locus_tag="ACHKA1_003428"
     CDS             5471..6475
                     /gene="msrP"
                     /locus_tag="ACHKA1_003428"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO5681035.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLHSWFKGNDRPPAPAGKPLDFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            6476..7111
                     /gene="msrQ"
                     /locus_tag="ACHKA1_003429"
     CDS             6476..7111
                     /gene="msrQ"
                     /locus_tag="ACHKA1_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO5681036.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALIGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            7368..8018
                     /gene="zinT"
                     /locus_tag="ACHKA1_003430"
     CDS             7368..8018
                     /gene="zinT"
                     /locus_tag="ACHKA1_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO5681037.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            8361..8891
                     /gene="yodB"
                     /locus_tag="ACHKA1_003431"
     CDS             8361..8891
                     /gene="yodB"
                     /locus_tag="ACHKA1_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO5681038.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(9461..9550)
                     /locus_tag="ACHKA1_003432"
     tRNA            complement(9461..9550)
                     /locus_tag="ACHKA1_003432"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9514..9516),aa:Ser,seq:cga)
     gene            9644..10441
                     /gene="mtfA"
                     /locus_tag="ACHKA1_003433"
     CDS             9644..10441
                     /gene="mtfA"
                     /locus_tag="ACHKA1_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO5681039.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            10542..10617
                     /locus_tag="ACHKA1_003434"
     tRNA            10542..10617
                     /locus_tag="ACHKA1_003434"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10575..10577,aa:Asn,seq:gtt)
     gene            10929..18932
                     /locus_tag="ACHKA1_003435"
     CDS             10929..18932
                     /locus_tag="ACHKA1_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO5681040.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLVTQLVFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALKAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSTQTLSADSHSSATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQVLTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPPGEITVTDTAPQQLTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGIAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVYTAEATLPNGNNDTKMVNIAPDASNALVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSTSNTNDSGVAEVTLKGTVLGVHT
                     VEATLLNGNGYTTTVNIAPDASNAQVTLNIPAQQVVTNNSDNVQLTATVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDS
                     QPVTFVADKTSAQVVLQMSKDEITGNGVDNATLTATVKDQFDNEVNNLPVTFSSASSG
                     LTLTPGVSNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTAVPDRIIAGTPQNSSGSVITATVVDNNGFPVKGVTVSFTSRTKSAEMTNGGQAVTN
                     EQGKATVTYTNTRSSRETGARPDTIEASLENGSSTLSTSIQVDADASTAHLTSLYTLY
                     DTQLAGDDTTLYITVNDNYGNGVPLHQVTLSVSPSEGVTLSNNGINTTNHDGYLYASM
                     TATKAGVYQVTATLDNGDSMQHTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATV
                     ADTEGNAIANTEVTFTLPEDVRANFTLSDGGKAITDTEGKAKVTLKGTKAGAHTVTAS
                     MAGGKSGQLVVNFTADTLTAQVNLNVTEDNFIANNIGMTKLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNSYGVSDTKPVT
                     LIADAGTAKLAGFTASSGSFTASTTEGATLTASVTDAYGNPLEGIKVNFRGPATTLSN
                     TSVETDAQGKAEILVTSTIAGTKVVTANLAIAPTEAAMRTLTVKADIDSATITSLEMP
                     EGQVIIREPIAVKAHVDDQFGNPVADQLVTFSAEPSSFNMVISQDTVSTNRQGIAEVT
                     MTPGRYGSYTVKASLANGSFYEKDLVVIDLRLTLTSSSQLIGVNDPSGATLTVRLTHA
                     NGAPLSHELVTFSVTPEGATLSSQTATTNTSGEAQVVLTSNKVGTYAVTASIHSGVII
                     ETQTTVKVTGNPSTAHVASFIADPSTIPANNSDISTLKATVEDGSGNLVEGVNVNFVL
                     KSGSATLTSLTAVTDQNGLATTSVRGAMTGSVTVSAETSYGGAQTVDITLVAGPADAS
                     LSVLKNNRSSLKGDFTESAELHLVLHDLSGHPINVSEGMEFVQSGTNVPYVQVSAIDY
                     SKNFSGEYKATVTGGGEGIATLIPVLNGVHQAGLNTTIEFISAEARPMTGTVSVNGAT
                     LPAASFPSQGFTGAYYQLNNDNFAPGKTAADYAFSSTASWVDVDTSGKVTFKNVGDRN
                     AVIITATPRSGGAIYQTQVRVKGWWVNHGNNLMQLSQAENYCSNQVGNGYTLPRADLL
                     SNGHMRREIGSLYGEWGDMGNYMNEADFYSMVYWSSNSAGAGQQYIVSLETGTQNTYQ
                     THEFFYGACYKQI"
     gene            complement(19194..20246)
                     /locus_tag="ACHKA1_003436"
     CDS             complement(19194..20246)
                     /locus_tag="ACHKA1_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO5681041.1"
                     /translation="MFLASLLRHIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLLIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            20561..21877
                     /gene="shiA"
                     /locus_tag="ACHKA1_003437"
     CDS             20561..21877
                     /gene="shiA"
                     /locus_tag="ACHKA1_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO5681042.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            21979..23433
                     /gene="amn"
                     /locus_tag="ACHKA1_003438"
     CDS             21979..23433
                     /gene="amn"
                     /locus_tag="ACHKA1_003438"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO5681043.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYIRSGELPDE
                     NARKQGLFVYPSLTVTWDGGTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            23775..24491
                     /gene="yeeN"
                     /locus_tag="ACHKA1_003439"
     CDS             23775..24491
                     /gene="yeeN"
                     /locus_tag="ACHKA1_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5681044.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT         6789 a         5771 c         5569 g         6386 t
ORIGIN      
        1 gcacttattg atatattcat gaagattata atcacaaggg aatacataat gaaaagaaaa
       61 gttctggcaa tgctggtccc ggcgttatta gttgctggcg cagcaaatgc ggctgaagtc
      121 tataataaag atggtaataa actggatttg tacggaaaag tagcgggcct gcactacttc
      181 tctgatgatg ctggcagcga tggcgacatg tcatatgccc gtatcggttt caaaggtgaa
      241 actcagatcg ctgaccaatt cactggttat ggtcagtggg aatttaacat tggcgcaaac
      301 ggtcctgaaa gcgacaaagg taataccgca acgcgtctgg catttgcagg tttcggcttt
      361 ggtcagaatg gtactttcga ctatggtcgt aactacggtg tcgtatatga cgtagaagct
      421 tggaccgata tgctgccaga atttggtgga gatacctatg ctggcgctga caacttcatg
      481 aacggtcgtg ctaacagcgt agcaacctat cgtaacaatg gtttctttgg tcaagttgat
      541 ggtctgaact ttgcactcca gtatcagggt aacaacgaga aaagcggatt atttgatcaa
      601 gaaggttcag gtaacggtaa tggacgtaaa cttgctaaag agaacggcga cggttacggt
      661 atgtccactt cctatgactt tgactttggt ttaagtctgg gcgctgctta ctctaactct
      721 gaccgtaccg acaatcaggt tcacaaaggt actcataaca ctcgttatgg agatcgcttc
      781 gatgcaactg ctggcggtga aacagcagaa gcatggaccg ttggtgctaa atacgacgct
      841 aataacgttt acttagcagc tatgtatgct gaaacccgta acatgactgg ttacggtgat
      901 gctgatgcca tcgctaacaa aacccaaaac tttgaagttg ttgctcagta tcagttcgac
      961 tttggtctgc gcccgtccat cgcttacctg caatctaaag gtaaggattt aggcggtgta
     1021 aatagtgata acttcgacag ccagggtaat catcactaca ccaacaaaga tctggttaaa
     1081 tatgttgata ttggtatgac ctactacttc aacaaaaaca tgtccaccta tgttgattac
     1141 aaaatcaacc tgctggacaa cgacgacgac ttctacaaag aaaacggcat cgctaccgat
     1201 gatatcgttg ctgtgggctt agtctaccag ttctaatcag ccaactcgct gcggcgtgtt
     1261 actgtaaaac tgaacactag cctgagttcg tccctcatcg aactcaggct tttttattga
     1321 catttcatct ccctctgcgg cacacgcaat tgccaaagca tatcgataca ccaccttacc
     1381 ctgcccctcc tggcacaata tcttctacca gctactcttc cagccaacac cccaaataca
     1441 tttctttatg aattaatccg caacaaacac acttcccata tagaaatatt tgcaaaaata
     1501 gtgcatgaaa aaagcttttt ctctgccaat cctggatata aatattcatc agcaccacaa
     1561 tattccacag cactaaatct ctccccgcca ccccgtacct ctgataatgg tctaaaatca
     1621 ttgaggccac ttgcgacgac gttactcaat aaggaaaatt tggcgtgaat aatattcaac
     1681 accagtgcag catcatcatt acatacttac acccgccgac taccgctttc gatatattga
     1741 ctcaattttc tagccgggtt aaaaatagcg accagctcag tcgcaaatat agtgactacc
     1801 ctaactaagc aacaataagg aatacactat gactgttcaa acaagtaaaa atccgcaggt
     1861 cgatattgct gaagataatg cattcttccc ttcagaatat tcgcttagcc aatataccag
     1921 tcctgtctct gatcttgatg gcgtggacta tccaaaaccg tatcgcggta aacataaaat
     1981 tctggtgatc gccgcggacg aacgttattt gccgaccgat aacggaaaac tgttctcgac
     2041 cggtaaccat ccgattgaaa cgttgctgcc gttgtatcat ctccatgctg caggtttcga
     2101 attcgaagtg gcgaccattt ccggtctgat gaccaagttt gaatactggg ctatgccgca
     2161 caaagatgaa aaagtaatgc cattctttga gcagcataaa tcgttgttcc gcaatccgaa
     2221 gaaactcgcg gatgtcgtcg ccagcctcaa cgctgatagc gaatatgcag caatctttgt
     2281 tcctggtggt catggcgcac ttattggttt acctgaaagc caggacgtgg ctgccgcttt
     2341 acagtgggca atcaaaaatg accgttttgt tatctccctt tgccacggcc cggcggcttt
     2401 tctggcgctt cgccacggcg ataacccact gaatggttat tccatttgcg cattcccaga
     2461 cgccgcagac aaacaaacgc cagagattgg ctatatgccg ggtcatctca cctggtactt
     2521 cggcgaagaa ctgaagaaaa tgggcatgaa tatcattaat gacgacatca ccgggcgagt
     2581 acataaggac cgtaaacttc tcaccggcga cagtcctttt gcagcgaatg cgttgggtaa
     2641 actggcggcg caggaaatgc tggcagctta cgcgggttaa tccctgttac gtttgaatgt
     2701 aagcgcataa tcaatcgcag ccgcgttagt actcacgacg tggctgcgtt ttttcacggt
     2761 taatttatgg cgtactgaag ccctatgtta atttctttgc ggtaacgtaa tccggaacac
     2821 attatgctta ttgagatagt gatacgtagc acttccccca tgcaattcgg caatcgcttt
     2881 gactaaagaa agaccaagtc cctgacctac ggaatgacgc gaattatctc cccgccaaaa
     2941 tctacggaag agtttttcag gctcattaat tttcgttcca gggctggcga tatcaatatt
     3001 aagatagctg ttggtatcaa gaaaactggt tatatgaata cgcgattttt ctggcgaata
     3061 tcgaatagcg ttaacaataa gattcgataa cattcgttgc agtaaaattt tatccgcaaa
     3121 gatttgctga ttgcactcga cattaaagca aacctctttc tcgtctgaaa gatattcaag
     3181 atagtccaac aaattttcga cttccttatt gagagaaagc gagtccagtt tcaccaaaac
     3241 attgttttta tctgccctgg caagaaacag tatgttctct gttaaccgcg aaatattttc
     3301 cagctcttca atgtttccgg caattgtttt ttgatattca gcgatacttc tggtttgact
     3361 tagcgtaacc tgattctgac ccagtaatgc attaattggc gttctaagtt catgagcgag
     3421 atcgtcagca aactgactta gacgctcaaa atctttgact aaagcatgat gcattttatt
     3481 caacgcctgc ccaagaggtt ttagttctct cggtaatgcg ctaacctcaa caggctcccg
     3541 gctatcgtta taattcagcg cttccgttac accactcaac tttttgatct ctcgtaatcc
     3601 cgttctgatt aacagcggac ttaatactga gcaaagtaca atggcgacaa tgcaaattat
     3661 aatgctatta attttatact gttcaagcat gttatgtctg gctgaagcca atttagccac
     3721 agtaaccgtt aatgatggcg aaacttcatc aatattaatt cgtaaagcat caatttcagt
     3781 gtcattaata atgcttctgt aaatgccagc tgcgctgatt gtctcactag caggtatgtt
     3841 atttaacatg tcatcactga catttgtccg gttaacaatt ttattgatgc catcaccatg
     3901 aatgatcaag atatcctgac taacatccat catccggtta aagtatacag gtaacgtatc
     3961 tggatttacc ccatcaatta acaactgctt gatctgtgct gtccggttaa tgagtgttgt
     4021 atcatcgcgc catttcaact cacttgccag gccattatag agagtccaga caattccggc
     4081 gccagcaaca gacagcagca atataaaaag caaggttaaa cggacggtta tagataatct
     4141 tttcattatt tttttaccgc tacgaacgaa tatcccatcc cacggattgt ggcaattagc
     4201 ttttcaggaa aaggatcatc aacttttgcg cggagcctgc gaatggcgac atccaccgta
     4261 ttggtatcac tatcaaagtt gattccccaa atttcactcg caataaccgt tctggggata
     4321 atctcgccag ctctggaggc cagcagccaa agtaactgaa actccttgcg cgtcagtgta
     4381 atactgatat tgtccctgct cacactttga ctaacagagt ccattcttaa gccgctgatt
     4441 tctaatgttg aattcaaagc gtgatgttgt cttaattgtg cccgaaccct tgccagcaac
     4501 tcagaaaatg aaaaaggttt taccagataa tcatttgccc cactgtccag ccctctgact
     4561 ctgtcatcga cagaatccct tgcagtaagg caaataacag gggtttgctt tgctgttctt
     4621 agcgtttgta agatctgcca gccatccata cccggaagca taatatccag aatgatcaat
     4681 gcataatcat ccttcagcgc aagataaagc ccatctctgc catcagaaac ggcatcaatg
     4741 acataacccg cttcggaaag cccctgcgtt acccactctt gggtcctttg attatcttca
     4801 ataagtaaaa tcttcatgaa aataatatgc cataaatcat atgttataga aacagcctgg
     4861 ttcattacaa aattgtaatg ctgctgtaag gttaccctgg acgctttttc gctatcctca
     4921 aaactcattc acatgacaag gatataaaca tgttaaagcg ttatttagta ctctccgtag
     4981 taacggctgc attttcatta ccttctttgg tctatgctgc acaacaaaac attcttagcg
     5041 tgcacatttt gaaccagcaa acaggaaaac ccgctgccga cgtgacagtc actcttgaaa
     5101 agaaagcgga caacggctgg ttacaactta atactgccaa aacagataag gatggacgaa
     5161 ttaaggcact gtggcccgag caaactgcaa ctacgggcga ttaccgtgtc gtatttaaaa
     5221 ccggggacta tttcaagaaa caaaatcttg aaagtttctt ccctgagatc cccgttgaat
     5281 ttcatattaa taaagtgaac gagcattatc atgtgccttt attacttagc caatatgggt
     5341 attcaaccta tcgtggcagt taatgcataa atatgtaaaa gcagatctct gctactgtta
     5401 gggaggacac tccctgacag attaacagta aacggctctt gctggctaac gacaaaaagt
     5461 gtgatggctt atgaaaaaga atcaattttt aaaagaatca gatgttacgg ccgagtcggt
     5521 attctttatg aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcactttc
     5581 tttgccccac gctgcacatg ccgatctgca tagctggttt aaagggaacg atcgtccgcc
     5641 cgcccccgcc ggaaaaccgc tggacttcag caagcctgcc gcctggcaaa ataacctgcc
     5701 actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa
     5761 agccgatccc gccgctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag
     5821 cggcgaagtg gcaaaaccat tgaccctcga tcatgatgac ttaacccgtc gcttcccgct
     5881 ggaagagcgt atttatcgta tgcgctgcgt agaagcatgg tcgatggtgg tgccgtggat
     5941 tggttttcca ctgcacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt
     6001 cgctttcgaa acaatttatg caccggaaca gatgccaggc cagcaggacc gctttatcgg
     6061 cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct
     6121 cacactgatg accgtaggtg tttatggcaa ggcgttaccg ccacaaaatg gcgcgccggt
     6181 gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa
     6241 gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt
     6301 ttacgccaac gttaatccgc atgttgatca cccacgctgg tcacaggcta ccgaacgatt
     6361 tattggttca ggcggcatcc tcgatgtaca gcgccagcca acgctactgt ttaatggtta
     6421 cgccgaccag gtggcatcgc tgtatcgtgg cctggatctg cgggagaact tctaaatgcg
     6481 tctgacagca aaacaggtga catggctgaa agtttgcctg catcttgccg gattgttgcc
     6541 gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc cagtgaaaga
     6601 tattcagcat tttaccggtc gcactgcact gaaatttttg ctggcgacct tgttaatcac
     6661 acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt
     6721 atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg agctgggcgt
     6781 gaataatctg gcgttaatag gcaaggagtt aattacccgg ccttatttaa cgttaggaat
     6841 tatcagctgg gttattttgc ttgctttagc gtttacttca acccaggcga tgcagcgaaa
     6901 actcggcaag cactggcaac agttgcataa tttcgtctat cttgtcgcga tcctggcccc
     6961 aatacattat ctgtggtcgg tgaagattat ttcaccgcag ccgctcatct acgctgggct
     7021 ggctgtactg cttttagcct tacggtataa gaagttgcgt tcccttttta accggttacg
     7081 caagcaggtt cacaacaaat tatctgtata acttcatatt gccgacaaag tacgcgttat
     7141 ccctgccgga tgtggcgcga gcgccctatc cggcctacgc agatcgcatc aggttatgtt
     7201 tcgtttgtca tcaacctcaa attatcaaca caattcattt tatttcaata agatagataa
     7261 gtacacctga gaaaactatg ctctcatctc ctaagagttg ttgcattttg tcatatgtta
     7321 caatataaca ttacacatca tatacaatga ctctggagaa aactgttttg gcgattcgtc
     7381 ttcacaaact ggctgttgct ttaggtgtct ttattgttag cgctcctgcc ttttcgcatg
     7441 gtcatcactc acacggcaaa cccttaacag aggtcgaaca aaaagctgct aatggtgttt
     7501 ttgatgatgc caatgtacaa aaccgaacgc tcagtgactg ggatggagtc tggcaatccg
     7561 tttatccttt actgcaaagt ggcaaacttg accccgtctt tcagaagaaa gcggatgcag
     7621 ataaaactaa aacatttgct gaaattaaag attattatca caaaggttat gcaacagata
     7681 tcgagatgat tggcattgag gacggcattg ttgaattcca tagaaataat gaaacaacat
     7741 cctgtaaata tgattacgat ggatacaaaa tactcaccta taaatcaggc aagaaaggcg
     7801 ttcgttactt attcgaatgt aaagatcctg aaagcaaagc ccctaaatat atacaattta
     7861 gcgatcatat aattgcacca cgaaaatcat ctcattttca catttttatg ggtaatgatt
     7921 cacagcaatc tttattaaat gaaatggaaa actggccaac gtattatcca tatcagttga
     7981 gtagcgaaga agtggtcgag gaaatgatgt ctcattgagt attctcatga taacgcctcg
     8041 atgccgcttt agtaagtcat cataactgcc actggtccat ccacaaacgc cactgaacgc
     8101 aagctagcta cagacacgct catcactatg acgtgtctgt atattaataa gctaacccgc
     8161 attgagttaa ccaataacgg attccataca caatacggcc aacagagaaa gatacctgtg
     8221 ctcacgccat tgcttatatt ggctggttac aatgtgcact atcaattttt taaataacaa
     8281 attcttaaaa ccacatctgt ataaggaatt tttaaggttc gtgggatagc ttgactgtga
     8341 aaatcacagg agctacaaaa atgaaccgat tctcaaaaac tcaaatttat ttacattgga
     8401 taacgctgct tttcgttgca ataacctatg ccgcgatgga actccgtggc tggtttccta
     8461 aaggtagtag tacttatctg ctgatgcgag aaacacatta caatgcgggt atattcgttt
     8521 gggtgttaat gttttcacgc ctgattataa aacaccgtta tagtgatcct tctattgtgc
     8581 caccgccacc tgcctggcaa atgaaagcgg cttcgctaat gcacatcatg ctttatataa
     8641 ccttccttgc gcttcctctg ctggggattg ctttaatggc ttacagtgga aaatcgtgga
     8701 gtttccttgg tttcaatgtg tctccctttg ttaccccaaa cagcgaaatt aaagcactga
     8761 taaaaaatat tcacgaaacc tgggcaaata taggttactt tttaatcgca gctcacgctg
     8821 gcgcagcact ctttcatcac tacattcaga aagataatac tctgttacga atgatgcctc
     8881 gccgcaaata agtcgatccc ctgacaagga aatgtgaacc atggacatca acgcgtcata
     8941 aatgtttacg caagtgcaca atgcaccctt tccactatca acaaggagga gcagaaactg
     9001 tgtaaatcgt acaatggtaa gacttatcaa aaatacaggt taattttggt atttaaacct
     9061 tttttacttg ttagtatgat acaaaggctt tcaaaaaagc tgggaaattc aacaactctt
     9121 ggaaattatg gactttgtcc cgcggcttta atctgacggc cgcgttcctt ttttgactca
     9181 attattgacc ccttctctac gcaacttcag ttcccaccac caactttgcg gcagtttcat
     9241 aggatgaatg tctaaaagaa taatggtgag cgataagaaa acgactgaat aactgcacat
     9301 tttcgctcga aagcttcctg tcagatccat agcgaatcaa gtgctgaatg tcacagtatc
     9361 gaacagaaaa cagtgacgat ctaacccttc aagaatattc tacgattgtt ctgtttagga
     9421 aaagcaaggc gggaagtcgg gagataagtc attgataaag tggcggagag agggggattt
     9481 gaacccccgg tagagttgcc cctactccgg ttttcgagac cggtccgttc agccgctccg
     9541 gcatctctcc gttcagatgg ttgccatgat gccaggaaat ttggcatttt aacagtccct
     9601 gtccgtgcaa ttttgttcaa gtgacgagtt tgcgagcaaa acgatgatta agtggccctg
     9661 gaaagtacaa gaatcagcac atcaaactgc ccttccctgg caggaagcac tatcgatccc
     9721 ccttttaacg tgtctgacgg aacaggaaca aagcaaatta gtcactcttg ccgaacgttt
     9781 tttacagcaa aagcggcttg ttcctttaca gggctttgaa ctggattcat taagaagctg
     9841 ccggatagca cttctatttt gcctacccgt tctggagtta ggactggaat ggctggatgg
     9901 ttttcatgaa gtcttaattt atcctgcgcc atttgtggtc gatgatgaat gggaagacga
     9961 tatcggtctg gtgcataacc aacgtattgt tcagtcaggt cagagctggc agcaagggcc
    10021 tatcgttttg aactggttgg atatacaaga ttcttttgat gcttctggtt ttaacctgat
    10081 tattcatgaa gtcgctcata agctggacac ccgtaacggc gatcgcgcca gcggagttcc
    10141 ctttattccg ttgcgtgagg ttgctggctg ggaacacgat cttcatgctg caatgaacaa
    10201 cattcaggaa gaaatcgaat tggttggcga gaatgcggcg agcattgatg cttatgctgc
    10261 cagtgatcct gctgaatgtt ttgccgtact ttctgaatat ttctttagcg ccccagaact
    10321 ttttgctcct cgttttcctt cattgtggca acgtttctgc caattttatc aacaagatcc
    10381 tttgcagaga ctgcatcacg ctaatgatac agactcgttt tcggcgacga atgttcatta
    10441 attaacaact ttgcagatta attaaccaat tgaaatgact tatgaaattt agtgttgaca
    10501 gacaaggtac cgctaagtaa tatgcgcccc gttcacacga ttcctctgta gttcagtcgg
    10561 tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat
    10621 tctaaaaatt cgctttttta gcgcaatgtc actgacctta gttgaacatt gttttttaac
    10681 ggatagcggg ttttgacatc ttaagcgccc tcgaccttta tggttgaggg cgttttgcta
    10741 tggcgccatc accattttcc cctcgattat aaaacttgag taattcagta gtctcccctc
    10801 ttgcaactca cacccaaaac tgcctaacga aaagttatta attttcaatc atattgctat
    10861 caggatttac attttttcgc tgtgctagaa agggcgcatt tatgttagct cgttcaggga
    10921 aggtaagcat ggctacgaag aagagaagtg gagaagaaat aaatgaccga caaatcttat
    10981 gcgggatggg tatcaaacta cgccgcttaa ctgcgggtat ctgcctggta actcaacttg
    11041 tgttccctat ggctgcggca gcacaaggtg tggtaaacgc cgcaacccaa caaccagttc
    11101 ctgcacaaat tgccattgca aatgccaata cggtgcccta cacccttgga gcgctggaat
    11161 cggcccaaag cgttgccgaa cgtttcggta tttcggtggc tgagttacgc aaactcaacc
    11221 agtttcgtac gtttgctcga ggttttgata atgtccgcca gggtgatgaa ctggatgtcc
    11281 cggcacaagt tagtgaaaat aatttaaccc cgccaccggg taatagcagt ggcaaccttg
    11341 agcaacagat agccagtact tcacagcaaa tcggatctct gctcgccgaa gatatgaaca
    11401 gcgagcaagc ggcaaatatg gcgcgtggat gggcctcttc tcaggcttca ggcgcaatga
    11461 cagactggtt aagccgcttc ggtaccgcaa gaatcacgct gggcgtggat gaagatttta
    11521 gcctgaagaa ctcccagttc gattttctcc atccgtggta tgaaacacct gataatctct
    11581 ttttcagtca gcatactctc catcgtactg acgagcgtac gcagattaac aacggcttgg
    11641 gttggcgtca tttcactccc acatggatgt cgggcatcaa cttctttttc gaccacgatc
    11701 ttagccgtta ccactcccgc gccggcattg gcgcggagta ctggcgcgac tatcttaaat
    11761 taagcagtaa cggctatttg cgactgacaa actggcgcag cgcacctgaa ctggacaacg
    11821 attatgaagc acgcccggcc aatggctggg atgtacgcgc agaaggctgg ttacccgcct
    11881 ggccgcacct tggcggtaaa ctggtctatg aacagtatta tggcgatgaa gtggccctgt
    11941 tcgataaaga cgatcggcaa agtaatcctc atgccataac cgctggacta aactataccc
    12001 ccttcccgct gatgaccttt agcgcggagc aacgccaggg taaacagggc gaaaatgaca
    12061 cccgatttgc cgtcgatttt acctggcaac ctggcagcgc aatgcagaaa cagcttgacc
    12121 cgaatgaagt cgctgcacgg cgtagccttg caggcagccg ttatgatctg gtggatcgca
    12181 acaacaacat cgttctggaa tatcgcaaaa aagaactggt ccgcctgacc ctgacagacc
    12241 ccgtgacagg gaagtcagga gaagtgaaat cactggtttc gtcgctacaa accaaatatg
    12301 ccctgaaagg ctataacgtc gaagccaccg ctctgaaagc tgccggtggt aaagtggtta
    12361 caacgggtaa agatattctg gttaccctgc cggcgtaccg gttcaccagt acgccagaaa
    12421 ccgataacac ctggccgatt gaagtcaccg ctgaagatgt caaaggcaat ttttcgaatc
    12481 gtgaacagag catggtagtc gttcaggcac ctacgctaag ccagaaagat tcctcggtat
    12541 cgttaagtac ccagacgttg agcgcggatt cccattcaag cgccacactg acttttattg
    12601 ctcatgatgc agcaggtaat cctgttatcg ggctggtgct ctcgacgcgt cacgaaggtg
    12661 ttcaggacat caccctgtcg gactggaaag ataatggtga cggaagctat acccaagtcc
    12721 tgaccacagg agcgatgtct ggcacgctga cgctgatgcc acagctgaac ggtgtggatg
    12781 cggctaaagc ccccgccgtg gtgaatatca tttctgtttc gtcatcccgg actcactcgt
    12841 caattaagat tgataaggac cgttatctct ccgggaatcc tatcgaggtg acggtagaac
    12901 tgagagatga aaatgacaaa cctgttaagg agcaaaaaca gcaactgaat accgcagtca
    12961 gcatcgacaa tgtgaaacct ggtgtcacta cagactggaa agaaaccgca gatggcgtct
    13021 ataaggcaac ctataccgcc tataccaaag gcagtgggct tactgcgaag ctgttaatgc
    13081 aaaactggaa tgaagatttg cataccgctg gatttatcat cgacgccaac ccgcagtcag
    13141 cgaaaattgc gacattatct gccagcaata atggtgtgct cgccaatgag aatgcagcaa
    13201 acaccgtctc ggtcaatgtc gctgatgaag gaagcaaccc aatcaatgat cataccgtca
    13261 cgtttgcggt attaagcgga tcggcaactt cctttaacaa tcaaaacacc gcaaaaacgg
    13321 atgttaatgg tctggcgact tttgatctga aaagtagtaa gcaggaagac aacacggttg
    13381 aagtcaccct tgaaaatggc gtgaaacaaa cattaattgt cagttttgtc ggcgactcga
    13441 gtaccgcgca ggttgatctg cagaagtcga aaaatgaagt ggtcgctgac ggcaatgaca
    13501 gcgccacaat gaccgcgaca gtccgggatg caaaaggcaa cctgctcaat gacgtcaagg
    13561 tcaccttcaa tgttaattca gcagcagcga aactgagcca aaccgaagtg aatagccacg
    13621 acgggatcgc cacagctacg ctgaccagtt tgaaaaatgg tgattatacg gttacggcct
    13681 ctgtgagctc tggttctcag gctaatcaac aggtgatttt tatcggtgat caaagtactg
    13741 cagccctgac cctcagtgtg cccccaggcg agatcaccgt caccgacaca gcgcctcaac
    13801 agctgacggc aaccttgcag gataaaaatg gcaacccact aaaagataaa gaaatcactt
    13861 tctctgtgcc aaacgacgtc gcaagtcggt tctcgattag caacagcgga aaaggcatga
    13921 cggatagcaa cgggattgca atcgcctccc tgaccggcac gttagcgggc acgcatatga
    13981 tcacggctcg tctggctaac agcaatgtca gcgatacaca gccaatgacg tttgtggcgg
    14041 ataaagacag agcggttgtc gttctgcaaa catcgaaagc ggaaatcatt gggaatggcg
    14101 tggatgagac gactctgaca gcaacagtga aagatccttt tgataacgtg gttaaaaatc
    14161 tgtcagtagt cttccgcacc tcccccgcag acacgcaact gagtctgaac gcgcgtaata
    14221 ctaatgagaa cggtattgct gaagttaccc ttaagggcac ggttttgggg gtttacacag
    14281 ccgaagccac actgcctaac ggcaacaacg atacgaagat ggtcaatatt gcgcccgatg
    14341 ccagcaacgc gctggtcacc ctgaacatcc cggctcaaca ggtggtgacg aataacagcg
    14401 acagcgtgca gctgacggcg acggtgaaag atccgtcgaa tcatccggtg gcgggaataa
    14461 cggtgaactt caccatgcca caggacgtgg cggcaaactt tacccttgaa aataacggta
    14521 ttgccatcac tcaggccaat ggcgaagcgc acgtcaccct caaaggcaaa aaagcgggca
    14581 cgcatacggt taccgcaacg ctgggtaaca ataatgccag cgatgcgcaa ccagtcacat
    14641 tcgtggcgga taaggacagc gcagttgtcg ttctgcaaac atcgaaagcg gaaatcattg
    14701 ggaatggcgt ggatgagacg actctgacag caacagtgaa agatcctttt gataacgcag
    14761 taaaagatct acaggtcacc ttcagtacca acccggcaga tactcaactt agtcagagca
    14821 caagcaatac taacgacagt ggtgttgctg aagttaccct taagggcacg gttttgggtg
    14881 ttcatacagt cgaagccaca ctgcttaacg gcaatggcta tacgacgaca gtcaatattg
    14941 cgcccgatgc cagcaacgcg caggtcaccc tgaacatccc tgctcaacag gtggtgacga
    15001 ataacagcga caatgtgcag ctgacggcga cggtgaaaga cccatcgaat catccggtag
    15061 cggggataac ggtaaacttc accatgccac aggacgtggc agcaaacttt acccttgaaa
    15121 ataacggcat tgccattacc caggccaatg gggaagcgca tgtcacgctg aaaggtaaaa
    15181 aagcgggcac gcatacggtt accgcaacgc tgggtaataa caataccagt gattcgcagc
    15241 cggtaacgtt tgtggcggac aaaacctcgg ctcaggttgt cctgcagatg tcaaaagatg
    15301 agatcacagg taatggcgtc gataacgcaa cgctaactgc aacggttaaa gatcagttcg
    15361 acaatgaggt gaacaatctt ccggtaacat tcagctcagc ctcttcagga ctcaccctga
    15421 ccccgggagt gagtaatacc aatgagtctg gcatcgcgca ggccactctc gcaggcgttg
    15481 cctttggtga gcagacggtc actgcatcac tggctaataa tggtgccagc gacaacaaaa
    15541 ctgtgcattt tattggcgac acagcggcgg caaaaattat cgagttgacg gctgtgccag
    15601 accgcataat cgccggtacc ccgcagaaca gctccggcag cgtcattacc gccacagtcg
    15661 ttgataataa tggctttccg gtaaaaggtg tgactgtgag ctttaccagc agaacaaaat
    15721 ctgccgaaat gacgaacggc ggccaagccg tgacgaacga acagggtaaa gctaccgtca
    15781 cttataccaa tacccgctcc tcgagagaaa caggagcgag accggatacc attgaagcta
    15841 gcctggaaaa tggtagctcc acgcttagca cgtccattca agtcgacgct gatgcgtcta
    15901 cggcacatct cacctcgcta tatacacttt atgacacaca attggcaggc gacgatacca
    15961 ctctgtacat tacggtgaat gataattacg gcaatggtgt acccctgcat caggtaaccc
    16021 tcagcgtttc gccgagtgaa ggcgtgaccc tcagtaataa cggtataaat actaccaacc
    16081 acgacggcta tttatacgca agcatgaccg ctacaaaagc cggggtttat caagtgacgg
    16141 caaccctcga caatggcgat tcgatgcaac acacagtgac ctatgtgccg aacgtcgcga
    16201 atgctgaaat cacgctggca gcctcgaagg atccggtgat tgccgacaat aacgatctca
    16261 cgacactaac agcaacagtc gctgatacag agggcaatgc gatagccaac actgaggtaa
    16321 catttactct gccggaagat gtgagggcga acttcacact gagcgatggc ggtaaagcga
    16381 ttactgatac tgaaggcaaa gcgaaagtca cgctgaaagg tacaaaagca ggcgctcata
    16441 ctgttacagc atcgatggct ggcggtaaga gcggacaact ggtggtgaac tttactgcgg
    16501 atacgctcac tgcgcaggtt aatcttaacg ttaccgagga caatttcatc gccaataaca
    16561 ttgggatgac caaacttcag gcaacagtga ctgatggaaa cggcaacccg ttagccaatg
    16621 aggcggtaac attcacacta ccggcagatg tgagcgcaag cttcactctc ggacaaggcg
    16681 gttccgccat tacagacatc aacggcaagg ctgaagttac actgagcggt acaaaatccg
    16741 gcacctatcc cgtgacagtt agcgtgaaca gttacggtgt cagtgatacg aagccggtga
    16801 ctttgattgc cgatgctggt accgctaaac tggctggttt taccgcatca tcgggctcat
    16861 ttaccgcgag cacaaccgag ggcgcgaccc tgacggcaag cgtcactgac gcttacggca
    16921 acccgctaga aggtatcaag gtcaatttcc gcggccccgc caccacgctg agtaacacca
    16981 gtgtcgaaac ggatgctcag ggtaaggcag aaatccttgt gacaagcacc attgctggaa
    17041 cgaaagtagt tacagctaat ttggcaatcg cacctaccga agccgccatg cgaacgctga
    17101 ctgtcaaagc agatattgat tctgcgacga tcaccagtct ggaaatgcct gaaggtcagg
    17161 taataatcag agaacccata gcggttaaag cacacgtcga cgaccagttt ggcaatccag
    17221 ttgctgacca acttgtgaca ttcagtgcag agccgtcaag ttttaacatg gtcattagtc
    17281 aggatacggt ttctacgaat aggcagggta tagctgaggt caccatgacg cctggaagat
    17341 acggttcgta tactgtgaaa gcatccctgg cgaatggctc cttttatgag aaggacctgg
    17401 tggtaatcga tctaagacta acactcacgt catcaagtca acttatcggc gtcaatgacc
    17461 catcgggagc aactctgact gtaaggttaa ctcatgcaaa cggggctcca ttgagccatg
    17521 aactcgttac ctttagcgta acgcccgaag gtgcaacgtt aagtagccaa acagcgacaa
    17581 ccaacacttc aggtgaggca caagtcgttc tgaccagcaa taaagtcggt acatatgcgg
    17641 tgacagcatc gatccatagc ggcgtaatta ttgagacaca gacaaccgtg aaggttactg
    17701 gcaacccaag caccgcacat gttgctagtt ttatcgctga tccgtcgact atcccggcca
    17761 acaatagtga tatcagcacg ctaaaggcta ctgttgagga tggcagtggt aacctggtcg
    17821 aaggggtcaa tgtaaacttc gtcttaaaaa gcggctctgc cacattgacg tcattaacag
    17881 cggtgaccga tcaaaacgga ctcgcgacaa cgagcgttag aggagctatg acggggagcg
    17941 tcacggtaag cgcagaaacg agctatggtg gagcgcaaac agtagatata acgctggtgg
    18001 caggcccggc agacgcttcg ctgtccgtcc ttaagaacaa ccggtcatca ctgaaagggg
    18061 actttaccga aagtgctgaa ctacatcttg ttctgcacga tctatcaggc catccgatca
    18121 atgtttctga agggatggaa tttgtgcaat caggtaccaa cgtgccctat gtgcaagtta
    18181 gtgcaattga ctacagcaaa aatttctccg gcgagtacaa agccactgtt acaggcggcg
    18241 gagagggtat cgcaacgctg atccccgtgt tgaatggtgt tcaccaggca ggtctgaata
    18301 ccacgataga attcattagc gcggaagcca gaccaatgac cggtacagtg tcagtcaatg
    18361 gtgcgaccct accggcagct tcattccctt cgcagggatt taccggggcg tattatcagt
    18421 tgaataatga caactttgca ccaggaaaaa cggcggctga ttatgcgttt tcaagcactg
    18481 cctcctgggt tgatgttgat acttccggta aggtgacatt caaaaatgtc ggagatagaa
    18541 atgctgtaat aatcacggcg accccaagat caggtggcgc tatttaccaa acgcaagtac
    18601 gggtgaaggg ctggtgggtg aatcacggta ataacctcat gcaattaagc caggctgaaa
    18661 actactgcag caaccaggtc ggtaatggct acacccttcc ccgcgcggac cttttaagca
    18721 atggacacat gcggcgcgaa atagggtcac tgtatggtga atggggagat atgggaaatt
    18781 acatgaacga agctgatttt tattcaatgg tatattggtc atctaattct gcaggcgcag
    18841 gccaacaata cattgtctcc ctggaaacag ggacgcaaaa cacttatcaa acgcatgaat
    18901 ttttttatgg ggcgtgttat aaacagattt aatttttcgt tataatttct taaagagtgc
    18961 tccattcggg gcactctttt ttacattccc tctatagagc ttgctcttct ctcaccgaaa
    19021 atgaataaat aaaaatgcga taccgccaga ttcgcgttcg gtgtgactat gatcgtatcc
    19081 ggtgagtaca gctttgatcc aaaggctggt tttctgtgat gattgaccag cctcagagta
    19141 ttgatgacac caccgttcat aacagatcgg cattactatg catacgtact gcctcaaatt
    19201 ctcgccaaaa cactttttaa tgcctttgcg aattcatcca gacttatatt gtcaattttt
    19261 tgcccaacga cgactgactc cgaaacatcg gccattgcgg gccatcgcgt tttgtaatca
    19321 gcataaattg caacaacggg ggtattatag gcacttgcca tatgtaatat tgaggtgtca
    19381 ggagttattg cgatataagc atcttttatg attgctgctg aacgtttaat cgagggtgat
    19441 aaggataaac ggtagacatt attataacaa tccactaata ccctggcttt atcttcccct
    19501 tttggtccat gaacgatcac tattggtata tctgtttcac tttgaatttt tgctattaga
    19561 ttttctgcaa tcaataatga aaatgtacgt tcctgcgagc taccttctaa attaagcgca
    19621 atgtaagatc ctaacgatcg catttcctcg cgcacctcat cgagtacatc ctcacttagt
    19681 ggcaattcat atattggcct gactgctgga aaacccgcct cacgcatcat aaatgcccat
    19741 gtcatgggta cgggagcccg gagattctga tccatccggg acgcgttctt acacacaggg
    19801 gaatagcact tcatggttaa accaacaacc tgaaaattcg tttttgcttt caactgactg
    19861 ataaataaca tcgttttcag gttcttttta cgcatcgcct ctatacaaag atctggcgta
    19921 ccgtattgct gtgttatgtt cttcgctaaa tcttttattt cttttaatgt tgcgtgatcc
    19981 tgcatagtca ttgtgactaa tgttaattta gtctgttcaa gtttgagcgc attaaagact
    20041 tctaaattaa ttgtcgacgt aacaattagt agatgcttaa ttttatgcaa ttcaagcgcc
    20101 cgaataacag gaaagatggc catagcatcg ccaatctgat cgggaatatg gatgacaaca
    20161 aaatctgttt tttcaatatt gaaattataa gctttataat cgtagtaact aaatgcaata
    20221 tgtctcaaca atgatgctaa aaacatacct aacctcgcct ccctactggt tataatgcaa
    20281 tgcagtctat cagactcatc agggtgccat tttgtgcata tgcggacttt tatgtttcat
    20341 atctcgaacc tgtgggtcct ctgcttaatc cttaaacaac accagcaact cccgcgcttt
    20401 catcttccag cgaatttttc atgttgccgc taatcagcca taaaatcatt tgcagatgcg
    20461 ctctgtcgag gtagtctcat aaggttcgtt tatagatcga cggcaatgtg agttaccttt
    20521 tccatactaa ttataaaaag acagtacaaa caggatcatt atggactcca cgctcatctc
    20581 cacccgtccc gatgaaggga cgctttcgtt aagtcgcgcc cgacgagctg cgttaggcag
    20641 tttcgctggt gccgtcgtcg actggtatga ttttttactc tatggcatca ccgccgcact
    20701 ggtgtttaat cgcgagtttt tcccgcaagt aagcccggcg atgggaacgc tcgccgcatt
    20761 tgctaccttt ggcgtcggat tccttttccg tccgctcggc ggtgtcattt tcggtcactt
    20821 tggcgaccga ctgggacgta agcgcatgtt aatgctgacc gtctggatga tgggtatcgc
    20881 gacagccttg attggtattc ttccttcatt ctcgaccatt gggtggtggg cacctatttt
    20941 actggtgacg ctgcgtgcca ttcagggatt tgccgtcggc ggcgaatggg gaggcgcggc
    21001 gttgctttcc gttgaaagtg caccaaaaaa taaaaaagcc ttttacagta gcggtgtaca
    21061 agttggctac ggtgtaggtt tactgctttc aaccggactg gtttcattga tcagtatgat
    21121 gacgactgac gaacagtttt taagctgggg ctggcgtatt cctttcctgt ttagcatcgt
    21181 actggtactg ggagcattgt gggtgcgcaa tggcatggag gagtccgcgg aatttgaaca
    21241 acagcaacat tatcaagctg ccgcgaaaaa acgcatcccg gttatcgaag ctctgttacg
    21301 acatcccggt gctttcctga agattattgc gctacgactg tgcgagttgc tgacgatgta
    21361 catcgttacc gcctttgcac ttaattattc aacccagaat atggggttac cgcgcgaact
    21421 tttccttaat attggtttgc tggtaggtgg attaagctgc ctgacaattc cctgttttgc
    21481 ctggcttgcc gatcgttttg gtcgccgcag ggtttatatc acaggcgcgt tgatcggaac
    21541 gttgagcgca tttcctttct ttatggcgct tgaagcacaa tctattttct ggatagtttt
    21601 cttctccata atgctggcaa acattgcgca tgacatggtg gtgtgtgtgc aacaaccgat
    21661 gtttaccgaa atgtttggtg ccagttatcg ctatagtggt gctggagtcg gttatcaggt
    21721 tgccagtgtg gttggcggtg gatttacacc ttttattgcc gctgcgctca tcacttactt
    21781 tgccgggaac tggcatagcg tcgccattta tttgctggct ggatgtctaa tttccgcaat
    21841 gaccgctttg ttgatgaaag acaatcaacg cgcttgatag tctggcgaag atcatccgat
    21901 cttcgcctta cacttttgtt tcacattcct gtgacatact atcggatgtg cggtaattgt
    21961 atggaacagg agacacacat gaataataag ggctccggtc tgaccccagc tcaggcactg
    22021 gataaactcg acgcgctgta tgagcaatct gtagtcgcat tacgcaacgc cattggcaaa
    22081 tatattagaa gtggcgaatt acctgatgaa aacgcccgca aacaaggtct ttttgtctat
    22141 ccatcactga ccgtaacctg ggacggtggc acaaccaatc cccccaaaac gcgcgcattt
    22201 ggtcgtttta ctcacgcagg cagctacacc accacgatta ctcgccctac tctctttcgt
    22261 tcgtatctta atgaacaact tacgttgctg tatcaggatt atggtgcgca tatctcggtg
    22321 caaccctcgc agcatgaaat cccttatcct tatgtcatcg atggctctga attgacactt
    22381 gatcgctcaa tgagcgctgg gttaactcgc tacttcccga caacagaact ggcgcaaatt
    22441 ggcgatgaaa ctgcagacgg catttatcac ccaactgaat tctccccgct atcgcatttt
    22501 gatgcgcgcc gcgtcgattt ttccctcgcg cggttgcgcc attataccgg tacgccagtt
    22561 gaacattttc agccgttcgt cttgtttacc aactacacac gttatgtgga tgaattcgtt
    22621 cgttggggat gcagccagat cctcgatcct gatagtccct acattgccct ttcttgtgct
    22681 ggcgggaact ggatcaccgc cgaaaccgaa gcgccagaag aagccatttc cgaccttgca
    22741 tggaaaaaac accagatgcc agcatggcat ttaattaccg ccgatggtca gggtattact
    22801 ctggtgaata ttggcgtggg accgtcaaat gctaaaacca tctgcgatca tctggcagtg
    22861 ctacgcccgg atgtctggtt gatgattggt cactgtggcg gattacgtga aagccaggcc
    22921 attggcgatt atgtacttgc acatgcttat ttacgcgatg atcacgttct tgatgcggtt
    22981 ctgccgcccg atattcctat tccaagcatt gctgaagtgc aacgtgcgct ttatgacgcc
    23041 accaagctgg tgagcggcag gcccggtgag gaagtcaaac agcggctacg tactggcact
    23101 gtggtaacca cagatgacag gaactgggaa ttacgttact cagcttctgc acttcgtttt
    23161 aacttaagcc gggccgtagc aattgatatg gaaagtgcaa ccattgccgc gcaaggatat
    23221 cgtttccgcg tgccatacgg gacactactg tgtgtttcag ataaaccgtt gcatggcgag
    23281 attaaacttc ccggccaggc taaccgtttt tatgaaggcg ctatttccga acacctgcaa
    23341 attggcattc gggcaatcga tttgctgcgc gcagaaggcg accgactgca ttcacgtaaa
    23401 ttacgaacct ttaatgagcc gccgttccga taataagttc tgaaccttct tatcaaaaat
    23461 agatgtgaag gattaagtaa gacatctggc aaatagcctg caataaagca ggctgtttgc
    23521 ttccttaggc ggataggagt tttattatcg tcttaatgat ttttacatat taaaagcaag
    23581 tatgctttca aaacacaatt atataaaatc ccgccaacaa tataagtttt tataaaatta
    23641 aatatgagat tatgacttta gaatattttt atttctaata gacgagattt ttcctgttat
    23701 gatataatat gccgaattaa cacatgttaa cgatttacca gtaatgtaaa taaattttcg
    23761 aggagatcat tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa acggctaaag
    23821 acggtgcaac gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct gctgctaaac
    23881 aaggtgaacc cgatccagaa ttaaacacat ctttaaaatt cgttattgaa cgtgcaaaac
    23941 aggcacaagt tccaaagcac gttattgata aagcaattga taaagccaaa ggcggcggag
    24001 atgaaacgtt cgtgcaggga cgttatgaag gctttggtcc taatggctca atgattatcg
    24061 ccgagacatt gacttcaaat gttaaccgta cgattgctaa cgttcgcaca attttcaata
    24121 aaaaaggcgg caatatcgga gcggcaggtt ctgtcagcta tatgtttgac aatacgggtg
    24181 tgattgtatt taaaggaaca gaccctgacc atatttttga aattttactt gaagctgaag
    24241 ttgatgttcg tgatgtgact gaagaagaag gtaacattgt tatttatact gaacctactg
    24301 accttcataa aggaatcgcg gctctaaaag cagctggaat cactgagttc tcaacaacag
    24361 aattagaaat gattgctcaa tcagaagttg agctttcccc agaagattta gaaatctttg
    24421 aagggcttgt tgatgccctt gaagatgacg acgatgtaca aaaagtttat cataacgtcg
    24481 caaatctcta attatatttt aaagaaatct gtctt
//
LOCUS       DAZFKF010000073        23940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFKF010000073 DAZFKF010000000
VERSION     DAZFKF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.073"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..339
                     /locus_tag="ACHKA1_003440"
     CDS             <1..339
                     /locus_tag="ACHKA1_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5681045.1"
                     /translation="NYREKITLEQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMT
                     EAKWLLTNTELSQAEISWRVGYENVDHFGKLFLRHVGCSPSDYRRQFKNCFAEQEILS
                     EFPQPVSLAG"
     gene            complement(382..1533)
                     /locus_tag="ACHKA1_003441"
     CDS             complement(382..1533)
                     /locus_tag="ACHKA1_003441"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO5681046.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPEPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1547..3142)
                     /locus_tag="ACHKA1_003442"
     CDS             complement(1547..3142)
                     /locus_tag="ACHKA1_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO5681047.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3287..4045)
                     /gene="aroD"
                     /locus_tag="ACHKA1_003443"
     CDS             complement(3287..4045)
                     /gene="aroD"
                     /locus_tag="ACHKA1_003443"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO5681048.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4076..4942)
                     /gene="ydiB"
                     /locus_tag="ACHKA1_003444"
     CDS             complement(4076..4942)
                     /gene="ydiB"
                     /locus_tag="ACHKA1_003444"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO5681049.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4954..6219)
                     /locus_tag="ACHKA1_003445"
     CDS             complement(4954..6219)
                     /locus_tag="ACHKA1_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5681050.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6446..7660)
                     /locus_tag="ACHKA1_003446"
     CDS             complement(6446..7660)
                     /locus_tag="ACHKA1_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5681051.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(7760..8116)
                     /locus_tag="ACHKA1_003447"
     CDS             complement(7760..8116)
                     /locus_tag="ACHKA1_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO5681052.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8252..8359)
                     /gene="rprA"
                     /locus_tag="ACHKA1_003448"
     ncRNA           complement(8252..8359)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKA1_003448"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8545..9657)
                     /gene="ydiK"
                     /locus_tag="ACHKA1_003449"
     CDS             complement(8545..9657)
                     /gene="ydiK"
                     /locus_tag="ACHKA1_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO5681053.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            10046..13102
                     /locus_tag="ACHKA1_003450"
     CDS             10046..13102
                     /locus_tag="ACHKA1_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO5681054.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLMR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13099..13509
                     /gene="menI"
                     /locus_tag="ACHKA1_003451"
     CDS             13099..13509
                     /gene="menI"
                     /locus_tag="ACHKA1_003451"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO5681055.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGYDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13609..13797
                     /locus_tag="ACHKA1_003452"
     CDS             13609..13797
                     /locus_tag="ACHKA1_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO5681056.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14346..14714
                     /gene="sufA"
                     /locus_tag="ACHKA1_003453"
     CDS             14346..14714
                     /gene="sufA"
                     /locus_tag="ACHKA1_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO5681057.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14723..16210
                     /gene="sufB"
                     /locus_tag="ACHKA1_003454"
     CDS             14723..16210
                     /gene="sufB"
                     /locus_tag="ACHKA1_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO5681058.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16220..16966
                     /gene="sufC"
                     /locus_tag="ACHKA1_003455"
     CDS             16220..16966
                     /gene="sufC"
                     /locus_tag="ACHKA1_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO5681059.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPGLCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16941..18212
                     /gene="sufD"
                     /locus_tag="ACHKA1_003456"
     CDS             16941..18212
                     /gene="sufD"
                     /locus_tag="ACHKA1_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO5681060.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGVKRSPQAQQHLEQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLELAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSMLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18209..19429
                     /gene="sufS"
                     /locus_tag="ACHKA1_003457"
     CDS             18209..19429
                     /gene="sufS"
                     /locus_tag="ACHKA1_003457"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO5681061.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLEMLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPEDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19442..19858
                     /gene="sufE"
                     /locus_tag="ACHKA1_003458"
     CDS             19442..19858
                     /gene="sufE"
                     /locus_tag="ACHKA1_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO5681062.1"
                     /translation="MAVLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            20007..21011
                     /gene="ldtE"
                     /locus_tag="ACHKA1_003459"
     CDS             20007..21011
                     /gene="ldtE"
                     /locus_tag="ACHKA1_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO5681063.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21075..21311)
                     /gene="lpp"
                     /locus_tag="ACHKA1_003460"
     CDS             complement(21075..21311)
                     /gene="lpp"
                     /locus_tag="ACHKA1_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO5681064.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(21622..23034)
                     /gene="pykF"
                     /locus_tag="ACHKA1_003461"
     CDS             complement(21622..23034)
                     /gene="pykF"
                     /locus_tag="ACHKA1_003461"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO5681065.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(23042..23236)
                     /locus_tag="ACHKA1_003462"
     CDS             complement(23042..23236)
                     /locus_tag="ACHKA1_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681066.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            23591..23800
                     /gene="fumD"
                     /locus_tag="ACHKA1_003463"
     CDS             23591..23800
                     /gene="fumD"
                     /locus_tag="ACHKA1_003463"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO5681067.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         6237 a         5744 c         6032 g         5927 t
ORIGIN      
        1 aattatcgcg aaaaaatcac tttagaacag ttatcgaaaa aatttcgtgc cagcgtcagt
       61 tatatttgcc atgagtttac caaagagtat cgtatttccc ctattaacta tgttattcaa
      121 cggcgtatga cggaagcgaa atggttactc actaatactg aattatcaca ggcagagatc
      181 tcctggcgtg tgggttatga aaatgtcgat cactttggca aactgttttt gcgccatgtc
      241 ggctgttcgc ccagcgatta ccgcaggcaa tttaaaaact gttttgcgga acaagaaatc
      301 ctatctgaat ttcctcaacc ggtaagtctt gccggataaa tctggcggcg cagtttgtta
      361 agaagctgcg ccgcctgcag attatttgtt ctgatagtct ttcaggatct gccgacctgc
      421 tacgtaaatc ataatttcgt ctgtaccgcc gccgatacgt tcacaacgga catcacgcca
      481 gaagcgggag acgcgcgctt catcggtata gcccagaccg cccatgattt gaatcgcatc
      541 atcaatgact tccattgcgg tacgtgcgca atacagtttt gcaagcgccg cgctggtgcg
      601 cagtgactga tgctgatcgg cttgccatgc cactttcagc accatgttgc gcatgttgtc
      661 aatcttaatc gccatcagcg ccagtttttc ctggatcatc tggttatgac caatgggctt
      721 accaaaagcg atacgttggt tggcatagcg ggcggcatct tcaaaggcgc attcggcaaa
      781 gccggtgctg cgcgcggcgt tgatcaggcg ctccatctca aagttgtaca tcacattgag
      841 gaaacccatt ccttcttcgc ccaccatgtc gctctcttca acttccacgt tgtcgagata
      901 gacttcgcag gtgctgagca tatgccagcc gattttatgc agcgggttaa tcttaatgcc
      961 gggcttactg gagtcgaccc accacagggt gaaggctttt ttgggatctt tcggttcagg
     1021 atcgcgcgcc agcaccagca tatacgggta ctctttcgca ccggtaataa aggttttctg
     1081 tccgttgatg taaaccttgc cgtttttacg cgtgtaagtg gtagtggcac tgttgttgtc
     1141 tgagcctgcg cctggctccg tcaacgccag ggcataggcg ggatcacccg tttccagtgt
     1201 gctttctgcc gttttacgta gctgctctgc agaaccgaaa cggcgcatac tgtgaataca
     1261 ttgaccgttg gtaatcaaaa atgccggagc accgcatttt gacacttcca tcagtgccag
     1321 catttgggtg acgtaatccg caggtatacc accaaattct tccggcacgc caagcatgga
     1381 aataccgtta tccgccagtg cccgcataaa ttcacgcggg tatgtcccgt tttgatcgca
     1441 ggtgcggaaa tactcttccg gaaagttagt cgtaatcagt tcgcgaatac tggccagcag
     1501 cagttcttgt tcttcagtta aagaaaaatc catcgtgata ctcctgttaa tgagccgctt
     1561 caggcaggac aaaacccatc gccgcatcga taaataatct ttcgtccatc agtttgagtt
     1621 ctggcgaaat cactggggtg aaatccattt tgtcgagaat atctttttgt aaatcgacgc
     1681 caggggcgat ttcgattaaa tgcaggccgt cttctttcag cgtgaatact gcgcgctcag
     1741 tgatataacg aacatccagc cctcgctcga gagcgatttt tccgctgaaa gtaatttccg
     1801 gtagttcccg aataaatttc ttcacccgtc cttcctggac gatatttaat ttgccgtcgg
     1861 taatttctgt ttttaaactg cccgcagtta atgtgccgca gaaaatgatt ttcttcgaag
     1921 tggcactgat atcaataaat ccaccggtac ccatgatttt gccattgaat ttatgcacgc
     1981 cgacgttacc gtgctggtcg acttcagcaa aactcaaata acaaacatcc agaccaccac
     2041 cgtgataaaa atcaaactgg gacgtcatat ccagaatggc gcgggtattc acgttcgcgc
     2101 caaaggcgat accctgtgaa gtaattccgc caatcggacc tgtttctacc gtcagaataa
     2161 agtcatcagc acaaccttct tcccgggcga ccaggccaat gccgtcagca ataccgacgc
     2221 cgacattccc caccgcgcct ttacgcattt cgaataatgc gcgccgcgca actaatttac
     2281 gttggtttag gggcaggcta agtttggtac tgtcatcaag tgtgaagtca ccagaaataa
     2341 agcggttaac cggtgcaccg ccatacagtt gggtttgatc cggatcgacc accacaatat
     2401 ccaccagata acccggaata cggacagatt taggatgcag cgtggctttc ttaaccattt
     2461 tctgcacctg catcatcaca ataccgccgt tattgtgcac cgcttgggca ataaccaatg
     2521 cgtcgagata catcacctca tcttcaaaag tggcgtagcc ttcactgtcg caggtggtag
     2581 cgcgaatgaa agcaatatct ggcgcaatcg ctttgtaata gagatattct ttgttatcaa
     2641 actcgaccag tttaatcagg tcttctttag tgacttcatt cagtttgccg ccttgctggc
     2701 gtggatcgac aaatgtcccg atgccaatat cactaataat accaggctgg tgggctgcgg
     2761 cggcgcgtaa ggtttgtgta agtacacctt gtgggtagtt ataagcaata attttatttt
     2821 gttctgcgag ttcagaaata cgcggcgatt gtccccagtg accacataat gcccatttca
     2881 ccagaccttc ttgcgccaga ggactaatac cacggtcggc gcgatcgcca agccctgttg
     2941 gactaataat cgataaatta cgtggcgttt gagtctgttt atatttatca gcaagagcag
     3001 taattaacgt ggtggcttcc agaataccgc cgccagcgcc taacacacaa agtgttgctt
     3061 cgtcgggaat ataattcacc gcttcctgtg ccgacaggac cggcactcgt ccattaatac
     3121 gaggtggttt tacaggtttc attgttaatt cctgattcag gtgtgatttt ccctacgcca
     3181 gatggataat tagcgcacag agactcacga aatattttta ttaaatacgg ttgaatatat
     3241 ttttttagtt cggcggggga ggatattccc gccgaaatat tattgcttat gcctgatgta
     3301 aaatagttaa tactgtgcgc aaatcattta ccgagatttg ccctggcgca gaggcttttt
     3361 ttaccgcacc aaaagttgcc gccgacccaa atacttcacc agccagacga gaaattacgc
     3421 cagtttttgc cattgacatc gtgatgattg gacgatcggc atactgctcc tgcatctcca
     3481 gggtcgcggc aagcaacgtc agcacatcgc tggtactttg cggcatcagc gcaatcttag
     3541 gaatatcggc gtcgaaggac tgcattttgc gcagacgggc aatgatttct tcggcttccg
     3601 gcgttttatg gaagtcatgg ttggacatga caactttcac atcatgcgcg tgggcgtagg
     3661 cgacggtttc tttaacctga tcatcgccgg taaataactc cagatcgatc atatcaacca
     3721 ggccgctgtc gatggctgca cgattgagag caatataagc ctcggtggaa atcgcctgct
     3781 cgccgccttc tttggcactg cggaaggtaa acagcagcgg tttttctggc atggtttcac
     3841 ggagaatttt tgccgccgcc atgacagact ccacattgga gaggtcggca aagtggtcca
     3901 cacgccattc cagaatatca aagtccgctt cacgataggc gagagcttcg gatttcacgc
     3961 gggcgatatc tttcgccatc agcgagacga tgattttagg tgcgcccgta ccaatgacga
     4021 gatcttttac agttacggtt ttcatttttt accctttctg cacgcggtca gcctgtcagg
     4081 caccgaaccc catgacctgt ttaacatatt ccagagggaa atctttgcca gtccataacg
     4141 tgaactgttc agccccttgc cacaacaaca tgccgtatcc atcaatcgtt ttgcaaccag
     4201 cttgttgcgc ctgctgcaat aacttcgtca tatgcgggtt atacacgcat tcagtgacca
     4261 gaagtcccgg atgtaacaga ctgatatcat taaccaatga ttcattctca aggggtttca
     4321 tacccacttt tgtgccattg gttaaaatgt cggcggaagc cagggcttca gcaaaggctt
     4381 gctgatcggc gagatcggta accgtgacga cacaatcggt gttttcgtta acccgctgcg
     4441 cgaaggcgag ggctttatcg aagaactcat cccgacggtt aaagagttta atttctttta
     4501 aaccttcaat tgccccctgc gcgccaatcg ccgttgaggc acctccggcc cctaacagca
     4561 ccattgtttt gcctttgata tcaaaaccgc tctctttaat ggcgcgaata tggcccgtgc
     4621 cgtcggtgtt atagccgcgc aggtagccat catcattaac gatggtgttg atggcaccca
     4681 ccagtttggc tgccggcgtt aattcatcaa catattcaca cgccagttgt ttgttcggca
     4741 ttgatacacc agttccgcgc atttttagag cttttaatcc ttcaattgct ccgggaaagc
     4801 tatcgttatc cacttcgaag gccatatagg taaatggcaa tcccgctttt tctaaggctt
     4861 tattctgcat ttcaggcgat aaactgtggc ggataggata agccatcaac ccaatcaatt
     4921 cgtattttgc ggtaacatcc ataattaact cctttaacct ctatgcttaa ttgtgtttaa
     4981 ccgggtgcta aatttgcgct cgccaaaccg cacatcattc tcaggaataa taaaaacgcg
     5041 gtaatagcgg ataaaaacaa taattgcggt aatcagggcc agcagcgcga aagtaaaatc
     5101 gagaacaatg atatattgca ggccgatgtt cgacagataa ccggtaatca gcggaataac
     5161 aaagttagcc agtccaccca tcatcatata aatactggtg actttggctt tgctttttgg
     5221 gaaaaactct gacatgaccg aaacaccgag ctgtaaaatg ccgccagctg ctgagaaacc
     5281 gataacaaag gcgccggcat tacacaccag tggagaaggg tacaggtaga taatggctgc
     5341 tgttattgtt gccagtgcag agttaaatac attagcccag atgggccgga ccattttttt
     5401 cagtagtgcg gcaaaaataa agacacagac caacgagccc atactgtaat aagagatggt
     5461 ttttaatgcc tcagcttctg acataccagc aaaagccatc gcatatttgg gcatccacac
     5521 cacaatcaca taaaaggtcg agaatgcggc tacaccgaac agtaccgatg aaacaccttc
     5581 cagccagact aacggtttgc tgttcatttg cggtaattca ttagctacgc tggcgtccac
     5641 caactggctg gggaatttgc ttttcaacag catcagcgtg atcagtacaa acagaatacc
     5701 cggaataatc agcccatagc cgtaccagat attattgagc aacatatagc tcaccagcat
     5761 tgggtagaac atttgcccaa atgacaccat cgctttaacc agtatgaccg ccgaaccaga
     5821 ggctttcgga aagcattcca tgagcgcggg gtagccaccc gtatccagcg ctgagttagc
     5881 gatacctaca cacactgcca gaccgtaggc gagagttaaa ttcgggcaag cgggaatacc
     5941 aaagaagaat agcagataca ttattactgc cattaatatc accgcccgac gaccaaactt
     6001 atcggagatc acaccgaaga ataaaatact gatcaatcgc cccaaaccga taccggaaat
     6061 taagtaggca atgcccgcgt tgtcagtgga aaacttttcc gccagagatg acatattttg
     6121 ggcaagcgta ataacactaa tgccgtgcag gaagtagctg aagtaaatac aaagaacagc
     6181 caggataaat ggcgtgctga aagccttatt ttgagacatt ttcacagctc cttgctgaaa
     6241 gataatttcg ttaccgtacg tattgttctc cgacggttta cataattact atactgaaga
     6301 tgattttccc tgggtacatg gcataaagtt tatgtttcca ggaatattgc atgtcataat
     6361 ttgtttttga tcgcgatttt ccttagttag cgcagaaaga gttatttatc cgtaagatct
     6421 tgcatgacct gaaaagaata ttgcattacc cacccggagc gacattttcc tttagcgagt
     6481 gatgccgcgt ttttttgcgg ctgcgtaagc cgataaacag tgccagtaaa aagccgatgg
     6541 cagcgatggc ggtatcgaac cacataatat cggcaatact tctttgggac agatgagccg
     6601 taatcaacgg aatagtaaag gtcgcaatac tgcccgcact gtaatagatc cctgtagctt
     6661 tacctttagc gtaggggaaa cgttcagcca ttaacgtcag gccaatttgc acaacacctc
     6721 cagcagaggt aaaaccaatg acaaaagcaa atattatcac cacataaaat gtgggatgca
     6781 ggcagacggt aaacagagca ataaatgaga taaaggtgta cagcatcagt aatgttgttg
     6841 ggcgaacggt attacgaatg agtggagcgg taataaatac acaaagcagc gaacccacgg
     6901 tgtagatact gagtagtttg atcgacatag tgtatgacat gcctgcaaca aattgtccgt
     6961 actgtgccag ccactggcta accagataaa acgttgccat tgagatatag ccatataagg
     7021 tataactggc taaatcgata attgaacagc gatgttccgt agagctggtg gttttcttta
     7081 tgacaggtaa gcgacgaccc ggatgggggg ggaacgtaca acggtataaa aacagagcgt
     7141 taataaacat aatgcctgca gcaatcataa aggaccaacc gaaccacagt tcagcccaca
     7201 ctaacaggct aatgattagc ggtaataaaa attgtccgct ggaaacaaat gctttaatta
     7261 aaatattggc tgtcccaggt gagcgtggaa aagcttccat caaactggga taagtgcctg
     7321 catcgagaaa actgtttgcc attcccgcca gaaagccaaa aacataagcg ataatgatgt
     7381 tattggtctg caggatgcca aaaaagaagg ccatatagca gcacatcccg agcatgataa
     7441 aagggcggcg accaaagcga tcggataata atcctgcaaa aagcaagaca cttaatcgac
     7501 caatgcccag cgatgagata actatcgaga cacccgcggc attagtctgc caaagtgtct
     7561 ccagcgaggc catattcagg ctcatcaaaa tgacgcccat accatgcacc aggtaattaa
     7621 aatacaaccc aagtgcggta gggtaatagg gatttttcat aggttctact tagcaaaggt
     7681 gctttaaaaa gtgtagcgat tttgtgtcag tatccgttca ttattgttgt aacaacgaca
     7741 attccttttt gtggagaaat taacaaagcc gctccagatc gtgtgcatat aattcagcgg
     7801 ctaccgattg atagatcttc cagtcaataa aattgccatc cgggtagaca cgttgaaacg
     7861 cggttaagtc ggggaaaaaa cgcatggtgt tgttgccgat gcggttattt attttctcta
     7921 taatggcatg aagatgtttt tttcgttgct gacgcatagc caacagttgc tcgactacac
     7981 gatcaggaat ggcgcatttg ccattttccc actgacgcca gctttcactg tcaccggtct
     8041 gggcaatcca ggtcgcgcat tcatcaatag tcatggcaaa aatatggcgg agtgcctgga
     8101 gttcataagc gttcatcgtg taatggctgc atgaaataaa caataaaata taaataacat
     8161 tatcagcctg ctgacggctt gaagagagtc acagtatctt gtgcaacatt attggaaaga
     8221 gtgaggggcg aggtagcgaa gcggaaaaat gttaaaaaaa agcccatcgt gggagatggg
     8281 caaagactac acacagcaat tcgttgtttc actcagggga tttccatgct tataaatcaa
     8341 tatgttgatt tataaccgtg agctaatagt aggcatacgg actttttgcg tcgatcagat
     8401 tcgtctcatt agtttaaaaa ctgtaaagaa tttgcgacag gtactaccag ctgaatggcg
     8461 gtaagcagga aaatatgggg aagagcaagc aaatatcaat tagcggtggt taacaatcag
     8521 gcggcagagc cgcctgatga gaaattactt attcggtttc tcgatttcgc caagttcttc
     8581 gagaatttct tccggttggt ccgtcggcgg cgggacttct tccacccacg cggcaaacag
     8641 acgccaggaa acggctaaca gaaccggacc aataaacaga ccgatcatcc cgaaagcaat
     8701 caaaccacca ataacgccag agaggatcag gatcagcggt aaatcggcac ccatgcgaat
     8761 taacattggg cggatgacgt tatccagcgt gccaaccaca ccgctccaca ctaacaatac
     8821 cgttccccag gtggtatcgc cggtccagta gagccagata atcgccggaa tcagtaccgg
     8881 caacgggcca agctggacaa ggcaggagag gatcattaac accgttagca acgttgcata
     8941 aggtacgccg gatacggcga ggccgatacc gccaagcact gcctgtacta acgccgtcac
     9001 caccacaccc agcgccaccg cgcggatagc ctgtgccgcc agcagcacgg cggcatctcc
     9061 gcgaacgcct gccagacggg tcgcaaaatg gcgaatgcct tgtgccacct gttcaccgcg
     9121 ccagtacagc agggcactga agagaagcat cagcgcacaa tgcaccataa agcgcccgat
     9181 atgcgccgct tgcccaacga accaggtggt ggtggtgcca atataagggc ggactttcgc
     9241 catgatcgcc gtccccccca tatccagcaa gttgtgccag cctgcataca gcttcgcgcc
     9301 aatcaccgga atggtattaa gccacgccag atcgggtaac gtcatgtcac cgctggaaat
     9361 ggctttaatt agcgggccgc tgccgtcgac gatactgtta accagcaaag caatagggat
     9421 gataaacacc atcactaata acagcgtcat caccagaacg gcgagagagc ggcggccaaa
     9481 catgatcttt tgcaaacgta acaataccgg ccaggtggcg ataaccaccg taccggccca
     9541 tgcaaagccg agaataaagg gttgaacaat ccacagacat gccacaatca tgatggctaa
     9601 aaacagcacc gaaagcagaa tttgtgcgac atccctgggc tgacgaacat ttaccataac
     9661 tacttttcac cttttttgtg cgtcaaatcg tcgacgcgac gtgaacacgc gaaactcacc
     9721 tgcataatga tgagcaattt cagcggtttt taacaggccg attctgccct taattctgat
     9781 gggcgtgtta gaaaaaaatg tggtacaaca actcaggcaa cacgcaaacg atttactcgt
     9841 cgtatttcaa gttgcatatg ctgcggctac gttcgttcac cccagttact tactgttgta
     9901 agctcctgga gttcactcgc ttgccgcctt cctgcaactc gaaatccata gagtaaataa
     9961 acatcacctt tctgtgcgct attgcagccg cctgaaagaa cagtgtatcg acctacatca
    10021 cagaccgcag gaaagggtca atataatgat tccacagatt tcccaggcac ctggcgtcgt
    10081 tcaactggtg cttaattttt tgcaagagct ggagcaacaa ggttttaccg gcgatacggc
    10141 gacaagttat gccgatcgtc tgacaatgtc gaccgacaac agtatttacc aacttctccc
    10201 cgatgcggtg gtatttccgc gttcaaccgc agatgtggcg ctgatcgccc gtcttgccgc
    10261 gcaggaacgc tattcatcgc tgatctttac cccccgcggc ggcggcaccg gcactaacgg
    10321 tcaggcgctc aaccagggca ttattgttga tatgtcccgc catatgaacc gcatcatcga
    10381 aattaaccct gaagagggct gggtgcgcgt tgaggccggg gtgataaaag accaactcaa
    10441 tcagtacctg aaaccgttcg gctacttttt tgcgccggaa ctttcgacca gcaaccgggc
    10501 aacgctcggt gggatgatca ataccgatgc atccggtcag ggatcgctgg tctatggcaa
    10561 aacgtcagat cacgtacttg gcgtacgcgc ggtgttgttg gggggcgata ttctcgatac
    10621 gcaaccttta cccgtcgagc tggcggaaac gctgggtaaa tccaatacca caatcgggcg
    10681 aatttataac acggtttatc aacgttgccg tcagcaacgc cagttaatta tcgacaactt
    10741 ccccaaactt aaccgctttc ttaccggtta cgatctgcgt catgtcttta acgatgagat
    10801 gaccgagttc gacctgacgc gcattctgac gggttcagaa gggacgctgg cctttattac
    10861 cgaagcgcgg ctggatatta cgcgcttgcc taaagtgcgg cgtctggtga atgtcaaata
    10921 tgactctttt gactctgcgc tgcgtaacgc gccgtttatg gttgaggcgc gggcgctttc
    10981 ggtagagacg gtggactcaa aagtgctgaa tctggcgcgg gaagatattg tctggcattc
    11041 cgtcagcgag ttgattaccg atgtgcctga caaagagatg ctcgggctga acattgtgga
    11101 atttgctggt gatgatgagg cgctgattga tgagcgggta aatgcactct gtgcgcggtt
    11161 ggatgagctg atcgccagtc atcaagcagg tgtcatcggc tggcaggtgt gccgtgagct
    11221 ggcgggggtt gaacgtatct atgcaatgcg caaaaaagcc gttggtctgc ttggcaatgc
    11281 caaaggtgcc gctaagccaa ttccgtttgc tgaggatacc tgcgtaccgc cggaacatct
    11341 ggccgattat attgctgaat ttcgcgcgct gctcgacagc cacggcttaa gttatggcat
    11401 gtttggtcat gtcgatgctg gggtgttgca cgtccgtccg gcactggata tgtgcgatcc
    11461 gcaacaagag attttgatga agcaaatttc tgatgacgtg gtggcgctga ctgcgaaata
    11521 cggtggtttg ttgtggggcg agcacggcaa aggttttcgc gctgaataca gcccggcgtt
    11581 tttcggtgag gaactttttg cagaactgcg caaagtgaaa gcggcatttg acccgcataa
    11641 ccgactcaac ccagggaaga tttgcccgcc agaaggtctc gatgcgccga tgatgaaagt
    11701 ggacgcggtg aagcgcggta cattcgatcg gcagatcccc attgcggtac gccagcagtg
    11761 gcgcggtgcg atggagtgta acggcaacgg tttatgcttc aactttgatg cccgtagtcc
    11821 gatgtgtccg tcgatgaaga tcacccagaa ccggattcat tcaccgaaag ggcgcgcaac
    11881 gctggtgcgt gaatggctgc gtttgttggc ggatcgcggc gttgatccac tcaaactgga
    11941 acaagaactg cctgaatccg gcgtcagttt gcggacgtta attgcccgca cgcgcaatag
    12001 ctggcatgcg aataaaggcg aatatgactt ctcacacgaa gtcaaagagg cgatgtcggg
    12061 ctgtctggcc tgtaaagcgt gttcgaccca gtgccccatc aaaattgatg tgccggagtt
    12121 tcgctctcgt ttcctgcagc tctatcacac ccgttattta cgtccgctgc gcgaccacct
    12181 cgtcgctacg gtcgagagct acgcaccgct gatggcacgc gcgccgaaaa cctttaactt
    12241 cttcattaac cagccgctga tgcgcaaact ctcggaaaaa catatcggca tggttgattt
    12301 gccgctgctg tcggtcccct cgctacaaca acaaatggtg gggcatcgct cggcaaacat
    12361 gacgctggaa cagcttgaag ctctcaatgc agagcagaaa gcgcgcacgg tgttggtggt
    12421 gcaggacccc tttaccagct attacgatgc gcaagtggtg gcggattttg tccgtctggt
    12481 cgaaaaatta ggtttccagc ctgtgttact gccattttcg ccaaatggca aagcccagca
    12541 tattaaaggt tttcttaatc gttttgcgaa gacggcgaaa aagacggcgg atttcctcaa
    12601 ccgtatggcg aagctgggta tgccaatggt aggcgtcgat ccggcgctgg tactttgtta
    12661 tcgcgatgaa tataaactgg cactgggcga ggaacgtggc gagtttaacg tcttactggc
    12721 gaatgaatgg ctggcaagcg cacttgagtc acagccagtg gctacagtca gcggtgaatc
    12781 atggtatttc tttggtcact gtaccgaagt taccgccttg ccgggtgcgc cagcacaatg
    12841 ggccgcgata tttgcccgtt ttggcgcgaa actggaaaat gtcagcgtgg gttgctgcgg
    12901 catggcaggg acttacggac atgaagcgaa aaaccataaa aattcgctcg ggatctatga
    12961 gttatcctgg catcaggcta tgcagcgact gccgcgtaac cgctgtctgg cgaccggata
    13021 ttcctgccgt agtcaggtca aacgggttga aggcacgggg gtacgccatc ctgtgcaggc
    13081 tttactggag attattaaat gatatggaaa cgaaaaatca ccctggaagc actgaatgct
    13141 atgggtgaag gaaacatggt ggggttactg gatattcgct ttgaacatat tggttatgac
    13201 acccttgaag cgacaatgcc agtagactcg cggacaaagc agcctttcgg gttgctacat
    13261 ggaggtgcat ccgtggtact ggccgaaagt atcggttccg ttgccggtta tttatgtacc
    13321 gaaggtgagc aaaaagtggt tggtctggaa atcaatgcta accacgtccg ctcggcacga
    13381 gaagggcggg tgcgcggcgt atgcaaaccg ttgcatctcg gttcgcgtca ccaggtctgg
    13441 cagattgaaa tcttcgatga gaaagggcgt ttgtgctgtt cgtcacgatt gacgaccgcc
    13501 attttgtgac gttgtcacca gaaaagtgtg acgcatattg cttttggtca ttatctgcac
    13561 agtaaacggt ggtatactgt gcaggtcttg tctaaaagcg aggatgctat gtctactcaa
    13621 cttgatccca cccagctggc cattgaattt ttacgccgtg accagagcaa tctttcacct
    13681 gcccaatatc tcaaacgtct gaaacaacta gaacttgaat ttgctgacct gcttactctc
    13741 tcttctgctg aattaaaaga agaaatctac tttgcctggc gtctgggcgt gcattgatcc
    13801 tgacttaatg ccgcaagttc gccaaatcga cctggattaa cctccgtcca ggttcgatga
    13861 ccttcttaat actgcttatc tacccgcctg atattgttca aaaataatac taatcgcagt
    13921 ttgtgttaaa acggcgggtt agctttatga gattattctt atcgcccctt caagagctaa
    13981 gccactgaga gtgccggaga taagcgccgg atggggtaga aacccttaag cctgtgtcgc
    14041 acaggcttaa gggttttctt atttctgaac gctgcacttt tttacctatc tcttaaatag
    14101 tcattttcaa tgcaactttt tctttttcat cacagcctta atgctcctta gataataacc
    14161 attatctaac aatgagatac ctaattctta gcgtgcctgt taacccacac atcagggtct
    14221 atgcttatta aaagcaggca aaaagcatgt tttacggtaa agcccctgcg tttgctgggt
    14281 tgaactgata atcattatca ctaacatgct gttatacgct gaaagcgatg aagtgaggta
    14341 aatcgatgga catgcattca ggaaccttta atccacagga tttcgcctgg caaggcttaa
    14401 cgctgacacc cgcagccgct gtccacatcc gcgagctggt ggcaaagcag ccgggtatgg
    14461 tcggcgtgcg cttaggtgtg aagcaaacgg gctgcgcggg ctttggctat gtgctcgaca
    14521 gtgttagcga gccggacaaa gacgatctgc tgtttgaaca cgacggcgcg aagctgtttg
    14581 tcccgctgca agcgatgccg tttattgatg gcacggaagt cgatttcgtt cgtgaaggac
    14641 ttaatcagat attcaaattt cacaacccta aagcccagaa tgaatgtggc tgtggcgaaa
    14701 gctttggggt ataggcggta ctatgtctcg taatactgaa gcaactgacg atgtcaaaac
    14761 ctggaccggc ggcccgctga attataaaga aggattcttc acccagttag ccaccgatga
    14821 gctggcaaag gggataaacg aagaggtggt gcgcgcaatt tcggcgaagc gtaatgagcc
    14881 ggaatggatg ctggagtttc gtctaaacgc ctatcgcgca tggctggaga tggaagaacc
    14941 gcactggttg aaagcgcact acgacaagct gaattatcag gattacagct actactcagc
    15001 accatcgtgc ggtaattgtg acgacacttg cgcgtctgaa cctggcgcgg tgcagcaaac
    15061 tggcgcgaac gcctttttaa gtaaagaggt ggaggcggcg tttgagcagt tgggcgttcc
    15121 cgtgcgggaa ggcaaagagg tggcggtgga tgccattttc gactcagttt cggttgccac
    15181 tacttatcgc gaaaaactgg cggagcaggg aattattttc tgttcctttg gtgaggcgat
    15241 ccacgatcac ccggaactgg tgcgtaaata tctcggcacc gtggtgccgg ggaatgacaa
    15301 cttctttgcc gcgcttaatg cggcggtagc ctctgacggt acgtttattt atgtgcctaa
    15361 aggcgtgcgc tgcccgatgg aactttccac ctattttcgc attaacgcgg aaaaaaccgg
    15421 gcagtttgag cgcaccattc tggtggccga cgaagacagc tacgtcagct acattgaagg
    15481 ctgttccgct ccggtgcgtg acagctatca gttgcacgcg gcagtggtgg aagtcatcat
    15541 ccataaaaac gccgaggtga aatattccac ggtacaaaac tggttccctg gcgataacaa
    15601 caccggcggt attctcaact tcgtcaccaa gcgtgctttg tgcgaaggcg aaaacagcaa
    15661 aatgtcatgg acgcaatcag aaaccgggtc agcgattacg tggaaatatc ccagctgcat
    15721 tttgcgcggc gataactcca ttggtgagtt ttactcagtg gcgctgacca gcggtcatca
    15781 gcaagcggat accggcacca agatgatcca catcggtaaa aacaccaaat cgaccattat
    15841 ctcgaaaggg atctctgccg gacatagtca gaacagttat cgcggcttag tgaaaatcat
    15901 gccgacggca accaatgcgc gcaatttcac tcagtgcgac tcaatgctga ttggcgctaa
    15961 ttgtggggcg cataccttcc cgtatgtcga atgtcgcaat aacagcgcac aactggagca
    16021 cgaggcaacg acatcacgta ttggtgaaga tcaactgttt tactgcctgc aacgcgggat
    16081 cagcgaagaa gacgccatct cgatgattgt taacggtttc tgcaaagacg tgttctcaga
    16141 actgccgctg gaatttgccg ttgaagcaca aaaactcctc gccatcagtc ttgaacacag
    16201 cgtcggataa ggaataaaca tgttaagtat taaagattta cacgtcagcg tggaagataa
    16261 agctatcctg cgtggattaa gcctcgacgt tcgtcccggc gaagttcacg ccattatggg
    16321 gccaaacggt tcgggcaaaa gtaccttatc ggcaacgctt gccgggcgag aagattatga
    16381 agtgacgggc ggcacggttg agttcaaagg caaagatttg cttgcactgt cgccggaaga
    16441 tcgtgcaggc gaaggcatct ttatggcctt ccagtatccg gtggagattc caggtgtcag
    16501 taaccagttt ttcctgcaaa cggcacttaa tgcggtgcgc agctatcgcg gccaggaaac
    16561 gctcgaccgc tttgattttc aggatttgat ggaagagaaa atcgcgctgt tgaagatgcc
    16621 ggaagattta ttgacccgtt cggtaaacgt tggtttttcc ggcggcgaga aaaagcgcaa
    16681 cgatattttg caaatggcgg tgctggaacc ggggttatgc attcttgatg aatcggactc
    16741 cgggctggat attgacgcat taaaagtggt tgccgatggc gtgaactcgc tgcgtgacgg
    16801 caagcgctca ttcatcattg ttacgcacta ccaacgcatt ctcgactaca tcaagcctga
    16861 ttacgtccat gtgctatatc agggacgaat tgtgaaatcc ggcgatttca cgttggtcaa
    16921 acaactggag gagcagggtt atggctggct taccgaacag cagtaacgca ctgcaacagt
    16981 ggcatcattt gtttgaagct gaaggggtga aacgttcccc gcaagcacaa cagcatttag
    17041 aacaattgct gcgtaccgga ctgccgacac gtaaacatga aaactggaaa tatacgcctc
    17101 tggaagggct gaccaatagc cagtttgtca gcattgcggg ggagatatcc ccacagcagc
    17161 gtgatgcctt agcgttaacg ttagacgccg tgcggctggt gtttgtcgat ggacgttacg
    17221 tgcctgcgct gagcgatgcg actgaaggca gcgggtatga agtgagcatt aacgacgacc
    17281 gtcaggggtt acccgatgct attcaggcgg aagtgtttct gcatttgacg gaaagcctgg
    17341 cacaaagcgt gacgcatatc gccgtgaagc gcggtcaacg gccggcaaag ccattgctgt
    17401 taatgcatat cacccagggc gtggcaggtg aagaggtgaa cactgcccat taccgacatc
    17461 atctggaact ggcggaaggt gccgaagcaa cggtgatcga acattttgtc agccttaatg
    17521 atgctcgtca ttttaccggg gcacggttca ctatcaacgt cgcagcgaat gcccacttgc
    17581 agcatatcaa gctggcgttt gaaaacccgc tcagtcacca ctttgcccat aacgatttgt
    17641 tgctggctga cgatgccacc gcatttagcc acagtttcct gctgggtggc gcagtgttac
    17701 gacacaacac cagtacgcaa ctcaatggcg aaaacagcat gttgcggatc aatagcctgg
    17761 cgatgccggt caaaaacgag gtgtgtgata cccgtacctg gctggaacac aataaaggtt
    17821 tttgtaacag ccgacagttg cacaaaacta tcgtcagcga caaaggccgc gcggtattta
    17881 acggtttgat caacgtcgcg cagcacgcca tcaaaacgga tggtcagatg accaacaata
    17941 atctgctgat gggcaaactg gcggaagtgg atacgaaacc gcagctggaa atctatgcag
    18001 atgatgtgaa atgcagccac ggcgcgacgg tggggcgtat tgatgatgaa cagatgttct
    18061 atctgcgctc gcgcgggatc aatcagcagg atgcccagca gatgatcatt tacgcctttg
    18121 ctgccgaact gacggaagca ctgcgtgatg aggggcttaa acagcaggtg ctggcccgaa
    18181 tcggtcaacg gctgccagga ggtgcaagat gactttttcc gtcgacaaag tgcgggccga
    18241 ctttccggtg ctttcgcgtg aggtaaacgg tttgccgctg gcttatctcg acagcgccgc
    18301 cagtgcgcag aaaccgagcc aggtgattga cgccgaggcc gagttttatc gtcatggcta
    18361 cgcggcggtg catcgcggta ttcatacctt aagcgcccag gcgaccgaga aaatggagaa
    18421 cgtgcgcaag cgggcatcgc tgtttattaa tgcccgttcg gcggaagagc tggtgttcgt
    18481 ccgcggcacg acggaaggga tcaatctggt cgccaatagc tggggaaaca gcaacgtgcg
    18541 ggcgggcgat aacatcatca taagtcagat ggagcaccac gctaacattg ttccatggca
    18601 gatgctttgc gcacgcgttg gcgcagagct gcgtgtgatc ccgctcaatc ccgacggtac
    18661 gttgcaactg gagatgctac ctaatctgtt cgatgagaaa actcgcctgc tggcaattac
    18721 tcatgtctcc aacgtgcttg gcacagaaaa tccactggcg gaaatgatca cgcttgcgca
    18781 ccagcatggc gcaaaagtgc tggtggatgg cgctcaggcg gtgatgcatc atccggagga
    18841 tgttcaggcg ctggattgcg acttttacgt gttctccggg cataaactgt atggccccac
    18901 cggaattggc attctttatg tgaaagaagc cttgttgcag gagatgccgc cgtgggaagg
    18961 gggcggttct atgatcgcca ccgtcagcct gagtgaaggc actacctgga ccaaagcacc
    19021 atggcggttt gaagccggta cacccaatac cgggggcatc attggtcttg gcgcggcgct
    19081 ggagtatgtt tcggcgctgg ggcttaataa catagccgag tatgaacaga atctgatgca
    19141 ctacgcgcta tcacagttgg aatctgtacc ggatctcact ctctatggcc cacaaaacag
    19201 gcttggcgtt attgctttta atctcggtaa acaccacgcc tatgatgttg gcagttttct
    19261 cgataattac ggcattgctg tgcgtaccgg acatcactgc gctatgccat taatggccta
    19321 ttacaacgtc cctgcgatgt gtcgggcgtc gctggccatg tataacaccc atgaagaagt
    19381 ggatcgtctg gtgaccggat tgcaacgtat tcaccgtctg ctgggataac agggaggcac
    19441 tatggctgta ttgccggata aagaaaaatt gctgcgtaat tttttacgct gcgccaactg
    19501 ggaagagaaa tatctctaca ttattgagct gggccagcgt ctgccagaat tacgcgacga
    19561 agacagaagc ccacaaaata gcattcaggg ctgccagagt caggtgtgga ttgtcatgcg
    19621 ccagaatgcg cagggaatta ttgaattgca gggcgacagc gatgcggcga ttgtgaaagg
    19681 gcttattgcg gtcgtcttta ttctctacga tcagatgacg ccgcaggata ttgtcaattt
    19741 cgatgtgcgt ccgtggtttg aaaaaatggc gctcacccaa catctcaccc catctcgttc
    19801 acaaggtctg gaagcgatga ttcgcgcaat tcgcgccaaa gccgctgcac ttagctaaac
    19861 taaagggaca gctttcatcc ggttggctca cattgccgga tggtcaatgt ttacactgac
    19921 ctgcacagga gtgtttcggt ttcaggtgaa cataagcgcc atccgacgtt tcagcgtgag
    19981 tctccggcaa atacaggagg tttacaatga aacgcgcgtc tttgcttaca ctcacgctta
    20041 tcggcgcttt tagcgccatc caggctgcct gggcggttga ttatccgcta ccaccaaccg
    20101 gaagccgact ggttgggcaa aatcaaacgt atacggtgca ggaaggggat aaaaaccttc
    20161 aggccatcgc ccgacgtttt gatactgcgg caatgttgat ccttgaagcc aataacacta
    20221 tcgccccggt gccaaaaccc ggtacgacga taactattcc ttcgcaactg ttattacctg
    20281 atgcgccgcg tcaggggatt atcgttaacc ttgcagagct gcgcctttat tattatccgc
    20341 cgggagaaaa tatcgtgcag gtttatccga taggtattgg cttgcagggg ctggaaacgc
    20401 cggtgatgga aacgcgtgtg gggcagaaaa tccctaaccc aacctggacg cctacggcag
    20461 gcattcgtca acgttcgctg gagcgtggca ttaaattacc gccagtcgtt cctgccggac
    20521 caaataaccc gctaggacgt tacgcactgc gactcgcgca tggtaatggc gaatacctca
    20581 ttcatggcac cagtgcgccg gacagcgtcg gtttgcgcgt cagttcaggg tgtattcgca
    20641 tgaatgcacc ggatattaaa gccttgttct ccagcgtgcg gacgggaacg ccggtgaaag
    20701 tgatcaacga accggtgaaa tattccgtgg agcctaacgg gatgcgttat gttgaagtac
    20761 atcgaccact atcggcagaa gaacagcaga acgttcagac aatgccatac acactgccag
    20821 caggctttac gcaatttaaa gacaataagg ctgtagatca gaagttagtc gataaagcgt
    20881 tgtatcgtcg ggcagggtat ccggttgcgg tgagcagtgg agcaactccc acagccagca
    20941 atgcgccttc agtagagtca gcgcagaatg gtgaaccaga gcaagggaat atgttacgcg
    21001 cgacgcagta gtggtaaata gcagacaaaa aaaatggcgc acaatgtgcg ccatttttca
    21061 cttcacaggt actattactt gcggtattta gtagccatgt tgtccagacg ctggttagca
    21121 cgagctgcgt catctttagc agcctgaacg tcggaacgca ttgcgttcac gtcgttgctc
    21181 agctggtcaa ctttagcgtt cagagtctga acgtcagaag acagctgatc gattttagcg
    21241 ttgctggagc aacctgccag cagagtagaa cccaggatta ccgcgcccag taccagttta
    21301 gtagctttca ttattaatac cctctagatt gagttaatct ccatgtagcg ttacaagtat
    21361 tacacaaagt tttttatgtt gagaatattt ttttgatggg aaggcactta tttttgatcg
    21421 ttcgctcaaa gaagcatcga acgctgggtt ttagataaaa aaattgagag aaaacaggat
    21481 gcttccatcg gattcatctt agataaagtg agattatata gtgaaatccg atttgatttt
    21541 ttattgcttc tggttatcga ttaaataaaa aaagcgccca tcagggcgct tcgatataca
    21601 aattaattca caaaagcaat attacaggac gtgaacagat gcggtgttag tagtgccgct
    21661 cggtaccagt gcaccagaaa ccataactac aacgtcacct ttgtgtgcca gaccgctctg
    21721 cagagccagt tctttaccca gacggtagaa atcatcagta gaagtgatct ctttaaccag
    21781 ctgcggcaca acgcctttgc tcagtaccaa ctgatgagcc gttttttcgt tggtggtcag
    21841 tgccaggatg gtggcatccg ggaagtattt acgtactgcg cgagcagatt taccgccctg
    21901 ggtagcaacc acgatcagcg gagcatccag tttttcagca gtttcaacgg caccacggca
    21961 taccgcttcg gtaatgcgca gtttacggtt gtcattgttg aactcaagac ggctgttcat
    22021 cacgcggtcg gtacgttcgc agatggtcgc catgatagaa accgcttcca gcgggtattt
    22081 accttttgcg gattcaccag acagcatcac tgcgtcagta ccgtcgagga tggcgtttgc
    22141 aacgtcaccg gcttctgcgc gagtcgggcg tgggtttttg atcatggaat ccagcatctg
    22201 ggtcgcagtg ataacgactt tacgtgcacg gatacatttt tcgatcatca tcttctgggc
    22261 gaagataact tcttctaccg ggatttctac acccaggtcg ccacgcgcaa ccatgatgcc
    22321 gtcagaggct tcgaggattt cgtcgaagtt gttgaggcct tcctggtttt cgattttgga
    22381 gatgatgtgg atgttttcgc cgccgtgcgc tttcaggtgc tcacggattt cgataacgtc
    22441 agaacgctta cgaataaagg aagcagcaac aaagtctacg ccttgttcgc aaccaaagat
    22501 caggtcctgt ttgtcttttt cagccagtgc tggcagggca atggaaacgc caggcaggtt
    22561 cacacctttg ttttcaccca ggtcgccgtt gttcagcact ttacagataa ctttgttacc
    22621 ttcaatggca gtaacttcca taccgatcag accatcgtca accagtacgg tgttgccaac
    22681 agacaggtca gttgtgaaac cttcatacgt taccgcaacc atttcgctgt tgccgataac
    22741 agatttatca gtagtgaaag taaaggtctg accagctttc agagaaacgt cgttaccgcc
    22801 ttccagtttc atggtgcgga tttccggacc tttggtatca agcaggatag cggcggtttt
    22861 accagttttg ctcatcacgt tgcgcagatt ctgaatgcgc tgaccgtgtt ctgcatagtc
    22921 accatgagag aagttcagac gcataacgtt catgccagcg tccagcattt tagctaacat
    22981 ctcttcagat tcggttttcg gtccgatggt gcaaacaatt ttggtctttt tcatgacagt
    23041 cttagtcttt aagttgagaa ggatgggaga aacttgcttt ctgggcgcac tgccggaaag
    23101 cgaatccggt attacgaaag tggtggtgcc tcgctctaag gataggtgac atcgaaaaag
    23161 cgtgcagagg aatgtgtgct tgtgtctcag cccaacgtga gtgcctgttt ttgagtcgtt
    23221 gagttctaca gtccaaagtg ctgaaaccat tcaagagtca attggcgcgc attatacgct
    23281 gaaattaatc caaaaggaaa acaaaaacag cgtttcaaaa aatatacaac ttgttagaca
    23341 ataaattgtt atcaaagatt taacgttcca gggaaaaggt tacgcaaaaa cttcgctgtt
    23401 tgcgcaacgt ctggtgccgg ggttgtcact gtgaagataa gtgaaagttc atccggtgta
    23461 taaatacatc atcgctcaat tgctacagtt aaaataattt atcgcgttat ttgtctgatt
    23521 ttgtttgatt tttgtttaat tggttagatt gatatatgct gatagttggc atgataaata
    23581 ggtataaaga atgggaaatc ggacaaaaga ggatgaactg taccgagaaa tgtgcagagt
    23641 tgtcggtaaa gttgtgctgg aaatgcgcga tctggggcag gaaccaaagc atattgttat
    23701 cgcaggcgta ttacgtacag cattagccaa taaacgcatt cagcgcagtg agctggaaaa
    23761 gcaggcgatg gaaaccgtca ttaacgcact ggtaaaatga ccattcagga tgaggcgata
    23821 agcttcatcc tgatattctt gcttcagttg cggtccagtt aatcattcta aataacaact
    23881 gctgaaatta tacgaatttc gatatgtgtt ttaatttata acgataatat tatttttatt
//
LOCUS       DAZFKF010000074        23653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFKF010000074 DAZFKF010000000
VERSION     DAZFKF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.074"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            310..1209
                     /gene="yegS"
                     /locus_tag="ACHKA1_003464"
     CDS             310..1209
                     /gene="yegS"
                     /locus_tag="ACHKA1_003464"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO5681068.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIVLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDNIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1291..2064)
                     /locus_tag="ACHKA1_003465"
     CDS             complement(1291..2064)
                     /locus_tag="ACHKA1_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5681069.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2170..3210)
                     /gene="gatD"
                     /locus_tag="ACHKA1_003466"
     CDS             complement(2170..3210)
                     /gene="gatD"
                     /locus_tag="ACHKA1_003466"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO5681070.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIVHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3258..4632)
                     /locus_tag="ACHKA1_003467"
                     /pseudo
     CDS             complement(3258..4632)
                     /locus_tag="ACHKA1_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            complement(4636..4920)
                     /gene="gatB"
                     /locus_tag="ACHKA1_003468"
     CDS             complement(4636..4920)
                     /gene="gatB"
                     /locus_tag="ACHKA1_003468"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO5681071.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4951..5403)
                     /gene="gatA"
                     /locus_tag="ACHKA1_003469"
     CDS             complement(4951..5403)
                     /gene="gatA"
                     /locus_tag="ACHKA1_003469"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO5681072.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5413..6675)
                     /gene="gatZ"
                     /locus_tag="ACHKA1_003470"
     CDS             complement(5413..6675)
                     /gene="gatZ"
                     /locus_tag="ACHKA1_003470"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO5681073.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRTYRYGCAE"
     gene            complement(6704..7558)
                     /gene="gatY"
                     /locus_tag="ACHKA1_003471"
     CDS             complement(6704..7558)
                     /gene="gatY"
                     /locus_tag="ACHKA1_003471"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO5681074.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKKYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7858..8910)
                     /gene="fbaB"
                     /locus_tag="ACHKA1_003472"
     CDS             complement(7858..8910)
                     /gene="fbaB"
                     /locus_tag="ACHKA1_003472"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO5681075.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9167..10444
                     /locus_tag="ACHKA1_003473"
     CDS             9167..10444
                     /locus_tag="ACHKA1_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO5681076.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLITAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10441..11445
                     /locus_tag="ACHKA1_003474"
     CDS             10441..11445
                     /locus_tag="ACHKA1_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO5681077.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRGEFTDDTSMALCLADALLEREGNIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPAHDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREVV
                     "
     gene            11442..12407
                     /locus_tag="ACHKA1_003475"
     CDS             11442..12407
                     /locus_tag="ACHKA1_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO5681078.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12381..13127)
                     /locus_tag="ACHKA1_003476"
     CDS             complement(12381..13127)
                     /locus_tag="ACHKA1_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5681079.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13179..13997)
                     /locus_tag="ACHKA1_003477"
     CDS             complement(13179..13997)
                     /locus_tag="ACHKA1_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO5681080.1"
                     /translation="MQLRITSRKKLTALLCALVLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPGQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKLIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(14062..14862)
                     /gene="thiD"
                     /locus_tag="ACHKA1_003478"
     CDS             complement(14062..14862)
                     /gene="thiD"
                     /locus_tag="ACHKA1_003478"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO5681081.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14859..15647)
                     /gene="thiM"
                     /locus_tag="ACHKA1_003479"
     CDS             complement(14859..15647)
                     /gene="thiM"
                     /locus_tag="ACHKA1_003479"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO5681082.1"
                     /translation="MQVDLLSFAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAI
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVATGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            complement(16514..16795)
                     /locus_tag="ACHKA1_003480"
     CDS             complement(16514..16795)
                     /locus_tag="ACHKA1_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681083.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            complement(16809..18470)
                     /locus_tag="ACHKA1_003481"
     CDS             complement(16809..18470)
                     /locus_tag="ACHKA1_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO5681084.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKNPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCILSRPLRRRVNI
                     QAHKVIHPKTNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNESLFVLIYELDDENEVDDPEMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMMISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(18454..18810)
                     /locus_tag="ACHKA1_003482"
     CDS             complement(18454..18810)
                     /locus_tag="ACHKA1_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO5681085.1"
                     /translation="MARPPKVPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(18933..19115)
                     /locus_tag="ACHKA1_003483"
     CDS             complement(18933..19115)
                     /locus_tag="ACHKA1_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681086.1"
                     /translation="MALMNEREQNRLIRGLIRQRDAWKAQETGHKDKASGRAERITAT
                     RLNDRDREVMECFRNR"
     gene            complement(19099..19539)
                     /locus_tag="ACHKA1_003484"
     CDS             complement(19099..19539)
                     /locus_tag="ACHKA1_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO5681087.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRSARQQQDSRRGSSRER
                     GYTRQWEKYRAMYLSKNPLCAHCLEKGIYTPAVVVDHIIPIDGGNDVLFWPEWNHQPL
                     CQACHNQKTKWLDPSTKNKRAAGGFREEEERAANRNNWMYGADE"
     gene            complement(19539..19835)
                     /locus_tag="ACHKA1_003485"
     CDS             complement(19539..19835)
                     /locus_tag="ACHKA1_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO5681088.1"
                     /translation="MTEELITLEEVKLHCRIDGDEEDQLISGYIAASLEACQIHIGRR
                     FDDGLEFTPAIKIGCMMFIAHLYENRQIVADNAKTHVPMTIGALWTAYRDVGVY"
     gene            complement(19832..20170)
                     /locus_tag="ACHKA1_003486"
     CDS             complement(19832..20170)
                     /locus_tag="ACHKA1_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO5681089.1"
                     /translation="MNIGRLRDRVTIQTLKQTRAMTGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVNTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(20167..21378)
                     /locus_tag="ACHKA1_003487"
     CDS             complement(20167..21378)
                     /locus_tag="ACHKA1_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5681090.1"
                     /translation="MWLFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVVSPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(21380..21952)
                     /locus_tag="ACHKA1_003488"
     CDS             complement(21380..21952)
                     /locus_tag="ACHKA1_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO5681091.1"
                     /translation="MGEKIMKSMEIRSSEITTSASNTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWVDLAGV"
     gene            complement(21992..23149)
                     /locus_tag="ACHKA1_003489"
     CDS             complement(21992..23149)
                     /locus_tag="ACHKA1_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5681092.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLTDESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKTREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            complement(23442..>23653)
                     /locus_tag="ACHKA1_003490"
     CDS             complement(23442..>23653)
                     /locus_tag="ACHKA1_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681093.1"
                     /translation="DEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHGFVRCA
                     LSGEMLAVNPEQIDALIEFLKGIRD"
BASE COUNT         5552 a         6334 c         5713 g         6054 t
ORIGIN      
        1 tgtacaacat ttcctacata agtaggaatt acggacatgg aggcccttcc gggtaactcc
       61 atgggggctt taatatatta tattgaagat gccactgttt agttgaatat taggtatatg
      121 ctcttttttg acatttatcg gtggctgccg ttagtattcg ctgtcccaat tgcaagctcc
      181 tggtggtaac cactgaatcc tccatacttg aactgacttt ttatcctccg actttcatcc
      241 tgttctgact ccaccttttc ttttctgctc tacactatct acagaccaat cataaaggca
      301 catacgatca tggcagaatt tcccgccagc ttactgattc ttaatggcaa aagtactgac
      361 aatttaccat tgcgcgaagc aattgtgctg ttgcgtgagg aaggaatgac gatccatgtg
      421 cgggtcacct gggagaaagg cgatgccgca cgatatgtag aggaggcccg gaagttgggc
      481 gtcgcaacgg tgattgccgg tggtggcgat ggcaccatta atgaagtttc tacggcgttg
      541 attcagtgtg agggggataa catacccgcg ctgggaattt tgccattagg aaccgccaat
      601 gattttgcca ccagtgtagg gattcctgag gcactggata aggcgctgaa actggcaatt
      661 gccggtaacg ccattgcgat agatatggcg caggtcaaca aacaaacctg ttttattaat
      721 atggcgacag gcgggtttgg gacgcgtatt accacagaaa cgccggaaaa attaaaagcc
      781 gcgctgggtg gcgtctctta catcattcat ggcttaatgc gcatggatac tctgcaaccg
      841 gaccgttgtg aaatccgcgg tgaaaacttt cactggcaag gtgacgccct ggtcattggt
      901 attggtaacg ggcgtcaggc cggtggcggt cagcaattgt gtccgaacgc gttaattaac
      961 gatggcttgc tgcaactgcg catttttacc ggcgatgaaa ttcttccagc tctcgtatca
     1021 accttaaaat ctgacgaaga taacccgaat attatcgaag gcgcttcgtc gtggtttgat
     1081 atacaagccc cacacgaaat cacttttaat cttgatggcg aaccgttgag tgggcaaaac
     1141 tttcatattg aaatacttcc ggcggcgttg cgttgtcgat taccaccaga ttgcccatta
     1201 ttgcgttaat caaactattc tctcttgaac cagagagaat ccgtaatgcc ggatgcggtg
     1261 taaacacctt atccggcata cagaacaata ctacaccgta attaatttta cccgtgtctt
     1321 ctgcaatgcc agtttatcca cctctgacac agcatcatcg gtaataataa tgtcaatttt
     1381 ttcgattggc agtacctgat taaaaccgcg acgattaaat ttcgatgaat cgagaacggc
     1441 aatcacttta ttagcagcgg tgaccatcgc accgctaatc gaataacctt cattaaaggt
     1501 cgtaatacca ttaaccgcat caataccatc agcaccgaca aacattaaat cggcattaat
     1561 atcctgcaat gaacgctcgg caatggaacc atgcatcgag cgcgttttat ggcggaccgt
     1621 gccaccgcag acgaccagag taatgtcttt attttcggaa agggcaaacg ctgccgggag
     1681 actgttggta atcaccgtga tatttttggc ggtcattaat ccttcggcga taagcattgt
     1741 cgtacttccg ctgtcgagaa taacggtcat cccttcgtgg atcattttga ctgccgcctg
     1801 cgctattctg ttttttggcg cgctggcgag ctgaaatcgc tctttaaacc cgacttcctg
     1861 ggtctcggtt tcactattac cggacattat tttcgccgca ccgccatgaa agcgcgtaac
     1921 cacgcctttt tgttcgagaa agcgcaaatc ggcacggatt gtcgcttccg aggcagcaaa
     1981 tactcccgcc agatcctgaa caagcacggt tccctgttca ttcactaatt ggatgatctt
     2041 attccttcgc tcgaatgaat tcatcgtcat ttacccatat tcaattgtgg ctagtgtaaa
     2101 cgaaggggga ccattggtga atgatctgtt ttgcacaata ccgggccagc atcacgctgg
     2161 cccgcggttt cagggaatga gcaacacttt gcccggcata gcattacgag cgatgtcacg
     2221 caccgcctgg gcgaagcttt caaagcttcc acggtgaacg attaatggct ctaggcttaa
     2281 cttacgttct gtcagcaacc ggctcgccgt ttcccactcc tgccccggcc aagggctgga
     2341 gtagttcatc caactgccga taaccgtcag ctctttacgc aatattttgc caaacgttgc
     2401 cgatgttaaa tgcagatcct gatgcaacgt ccccaccagc gccagttggg catgaggacc
     2461 ggcaatctct accgccagtt cgacagtttg cggtacgcca gccgtctcga ggataagctg
     2521 attaaagcgc agctcgcgta aaacgccctg catttgcggc gcgctcattt cgctactgtt
     2581 aaatgtttgc atcgcaccga aagattttgc cagtgctagt ttttctgagc taatgtcgat
     2641 cgccgtcaca ctctttgctc ccagcgcgac agcgcactga atagccagca ggccaatggt
     2701 tccggcacca ataataataa cgtttttatt ctcacaacct tgcgctaaat gaaaagcatg
     2761 caggccaacg gtaatcggtt caataaaagc cccatcctca ataggcatat ccgtgggtag
     2821 agcaaagaca tttttccgct taacgacaat atattcggca aatccaccat cacgccgtga
     2881 gccaataaaa tcatattttg cgcactggga ataaaaccct ttcagacact ctggacaagt
     2941 aaaacagggt aataacggca cacaggcaac cgcatcgcca gggtgtaaat catcaacacc
     3001 ggatcctacc gcatcaatat agccgctaaa ttcatggcct aacgttattg gataataatg
     3061 tgcaccattt ttaaatatcc tgggtaaatc ggaaccacat aagcccgagc tggcaatttt
     3121 tacccgcacc tcatcctgat gtttaatttc aggaatgacg ctttctgcaa cgcgcacgat
     3181 accatcagta tcattcacca ctgatttcat aaaaactcct gattgttaag ggggataacc
     3241 tccccctcag gtaaaaatta ttctgcaaga acgactttct cttgtttaat aaagccacgc
     3301 gctctacgcc aggtcatgaa aataccggta agataaattg cgccgataat aatgaaaccg
     3361 ggaatatttt gcggggagaa aacctgaatc agtaaccagg taatgggaga accgccctga
     3421 tccattgaag ccaccatacc cccggctttt aacgccccag cattagccgc cagttgggtg
     3481 tgcaaaccaa tagtttgcgt cgcgatccac agggtgatgc tcataatgat gacacccgag
     3541 attaaggtgc ggaacagatt tccacgatgc accgcgaccg ccatcgccac aaagaagccg
     3601 atagtggcaa gatcgccaaa cggcagcacc tgattacccg gcacacaaac agcaattaaa
     3661 atggtgagtg gaataaaaat caggcttgcc gataccaccg ccgtgtgccc cagcagcaac
     3721 gccggatcaa ggccaatcag gaactcctga ccgccgaact tcgcctgtaa acgactacgg
     3781 gcctgcttag cgatgggtgt taaaccatcc atgatgggtt aaaccatcca tgatgggttt
     3841 aatcacccgt ggcatcagca gcatcaccgc cgctgttttt accgccagct gcaatacacc
     3901 tttgacatcg taacccgcga ggatgccgat aatcagcccc atcacaaaac ccacggtgac
     3961 aggctcgcca aacgggccaa atttgcgctg aatatcgtcg gcgctaaatt taatacggtt
     4021 aacgcctggg attttctcga tgatagcatc gaccagcacc gcaattggcc ccatatacgc
     4081 cgacgtaccg tgcgggattg caatgccttc cagctcaaag aaatttcggg tatcgcgggc
     4141 aaaccagtcg ccgagcttat aaacaaacgc cgcgtgaatt accacacccg ccatccctat
     4201 catccatgaa ccggttgcca gatgcagcaa cgcgccggtg aaggtcatat gccagatatt
     4261 ccagatatca acatttacca cccgcgtcat acgggtcagt aacatcgcca cgttaaccag
     4321 aatcgcaatc ggaatcgcca ccagcgcaat ttgcgacgcc caggtcattg gtgaagagcc
     4381 cggccagccg acatcgacca catgcagatt caggtcgaaa ttttccgcca tcgctttcgc
     4441 cgccgggcca atggaatcca gcattaagcc aatcacaagg ccaatgccaa caaagccaat
     4501 cccgatatgc agacccgctt taaagcaatc gcctgccttc atgcctaaaa ttttagaaaa
     4561 aataatgatg acaatcggca acatcaccgt agggccgaga tcgagaatat aacgcatgac
     4621 ttctgaaaac ataggtcacc cctgtaagat agtcagaatt ttattttgta atgcttcgat
     4681 accgacacca gaaacaaaag gcatgccgtg aactaatgga atatcgccaa aactacgatc
     4741 cactttggca gtggtgcata tcaaatgcac gccatccata taggtttcta tttcattaac
     4801 ccgacactgg attaattcaa caggaatatt atgactctga cacaactctt taatttcttc
     4861 cgccgccatc gtagaggtcg caaccgcgcc tccgcaagcg acaataatct tgcgtttcat
     4921 aaatagtcct ttttattgtt acagagggat ttattcgtct gaatctaaaa catactttgt
     4981 gaagtattcc tttaactgag tttcaggaag agtgattagt gtctcgacga tatcgggctg
     5041 ttgtaacttg ccaaataaac agcgtaaaag tttcaattgc tgctgcggat tttccacaat
     5101 caacgcaata accaacgata ccgccacgtc attatcatca tccgcttgct gaaaatgaac
     5161 tttatttgtt ggccttaaca gataaatggc tgacgactta gcatgaatcg cctcacaatg
     5221 cggtattgca atggcgtgct gctcaagcat tatcccagta gggaattctg cttctctggc
     5281 aattaatgcc tgtggccagg tatcatgaac cacacctttc gcgagcatct cattaccgat
     5341 atgggttaaa acttcgctac gatcgacaaa agaaattccg ctacgaacaa acaggttagt
     5401 catataccgt ccttattccg cacagccgta gcgataggtg cgcaaaacat cataaatttt
     5461 atccataata agctgatgcg gtattgctga taattccccg gactgaatgc gttcaaattg
     5521 tttaggaaga tactgactaa tcatgcccag tgggatgtcc acgccttcca gattcaccat
     5581 catcgtttca acgctatttt taatccgact atgcggccag taataacgaa tacgatccga
     5641 taggctgtaa cgaatatcca gtaatgaatc gttaaaaccc gtgcgataat attttttcca
     5701 gtattgcggt tcgtcgagca tcacttcttc aattaccgcc aggcaaccgc tgcgattttc
     5761 gggggcgata agttcctgct caatttgtgc cagtgcaaat atcgcctcgc gtaaagcaaa
     5821 ggttaatgcg ggaccgactt tcaatattgc aaagtgatcg cggactaatt cccaataagc
     5881 cgtccgggtc tggtaatcgg tagaatgtgc ttcataaacc attcgggtgt tttctatcca
     5941 ttgcgccagc gcctgcgctt cctgcggctg ataatggata atattgctgt gatcaaattc
     6001 cacacccggc tgcaccacga tggcaatcac acgtgttaac gcctctgtca ggccacgggc
     6061 aataaaggcc ttttgatgcg tacgtaaagt attggcggca tcttcaacat gggtgatgtg
     6121 tactgactga atggcgctgg cctcaccgcc cggaaccgga acttcggtgc caatgacata
     6181 gctcagttgc tcacgctggc aatctgtcgc cacactttcc gcagcaaagc aaagcacagc
     6241 agctcgttcc gcaaccgttt ctggtgctaa cggtatggga tcccccgcgc aggacattga
     6301 cgcatcaaga tgaattttac tgaagccagc acgaacatat gcctttacca gctcgacgga
     6361 tttttccatt gccgcatccg cattttcttg ctgccagcag tttggcccca gatggtctcc
     6421 gccgagaata atgcgctcgc gcgcaaatcc gactttatcg gcaatcgtaa aaacaaattc
     6481 gcgaaagtct gccggtgtca ttccggtata accgccaaat tgattgacct ggtttgacgt
     6541 tgcttcaatc agcactttgc gcgtgctgtt gcgatcaaat gccagcgccg cttcgataac
     6601 caacggatgg gcagaacaga ctgaacatat gccgatatgt tcaccagctt tatgccgggc
     6661 aattaacgtt ttcatgtttt ttccttgtta aatggcaggt gcgttatgcc ctgccctcgc
     6721 agccacaatc ggcaatcact ttgctcacca catcgcgcat tgcggattta gccgactgca
     6781 aataatcccg gggatcggtc gcttcggggt gctcggtcag gtattttttt aacgcctgcg
     6841 agaaggcatt tttcagctcc gttgcaacgt tgattttgca tatccccagt ttgatggttt
     6901 gctgaatatc cttagtcgat aaccctgacg cgccatgcag caccagcggt aagttcaccc
     6961 actggcgaat gttctccagt cgagaaaaat caagcgccgg tgcgctggca tacataccat
     7021 gagccgtgcc gatggcgacc gccagggaat caattccggt tgcctcggca aattcacgcg
     7081 cctgagcggg gttggtataa aaagcatcgg cttcattgac ttgcacatca tcttcctggc
     7141 cgccaagttg ccccagctcc gcttcgacgc tgacatcaaa gcgatggcaa aaatccacca
     7201 cctctttgac ccgtgaaata ttttgcgcaa aaggcaaatg cgaggcgtca atcatgactg
     7261 agcgcacgcc agaacgaacc ttctgagcga tatcgtcaaa tttcgtgtga tggtcgagat
     7321 gaattgccag tggatggtga tattgcttcg ccatcgcgct gaccagcgcc aagagatttt
     7381 ctgtaccagc atgagtaaat gtgccaggcg ttccggcgat gatgaccggc gcatgcaggt
     7441 tggcagcggt ttctaccacc acttgcatcg tttcgagatt gtgaatattg aatgccggaa
     7501 ccgcataacc gccgcgctgt gcgttgttca gcatctgctt tgtcgatacc acgtacattt
     7561 tcatatcctg tcgtttgttt tcgatttcaa aatataatga aatgatttgt ttttaaatgt
     7621 cgagataacg atcacaaaaa cgacaatatg aaaattaata gaggaatgaa aggcaaaaaa
     7681 aaacggcctc ccgtagggaa gccgcaacaa agtgcgcgtg gttttatgca ggatgcggcg
     7741 taaacatatg tggcagcgct gcgtaggcat gataagacgc ttcagcgtcg catcaggcat
     7801 aggttgccgg atgcggcgta aacgccttat ccgtcctacg gggtagtgcc gttcgtatca
     7861 ggcgatagta attttgctat cgagataaac gtcctgcacg gcgttaatca gtttcacgcc
     7921 gtcagccatc gatttcttga acgccttacg tccaagaatc agccccattc cgccagcgcg
     7981 tttgttgata accgcagtac gcactgcatc actgaggtca gtttcaccgc ctgcagcacc
     8041 gccggagttt atcaacccgg cccggcccat atagcagtta gctaactgat aacgcaccag
     8101 atcaatcggg ttttcgctgg ttaacttgct gtacacgcga tcgtcggtat aaccgtaatt
     8161 aattgcttta tagccgccgt tattttccgc cattttttgt ttgacgatat ctgcacctat
     8221 ggttgccgcc agatggttcg cctgaccagt caggtcggcg gagacatggt aatcaacgcc
     8281 atctttctta aaggcggagt tacgcaaata ggcccacagc actgtcacca tgcccagctc
     8341 gtgcgcacgt tcaaaagccg cagaaatttc ttcaatctgg cgacgtgact cttccgagcc
     8401 aaaatagata gtcgcaccaa ccgccaccgc acccatgttg aacgcctgct ccacgctggc
     8461 atacagcgtt tgatcgtagg tattcgggta acttagcgtc tcgttgtgat taagtttgac
     8521 gagaaatgga atgcgatgcg cgtaacgccg cgataccgac gccaacacgc cgtaagttga
     8581 cgccacacag ttacagcccg cttcgatcgc cagttcaaca atgtttttcg ggtcaaagta
     8641 gagcgggtta gcggcaaatg aagctccagc agagtgctca acgccctggt caacgggcag
     8701 aatagaaaga tatcctgtgc cagccagacg cccggtgttg tacaacgtct gcatattacg
     8761 taacaccgct ggcgggcgat tattgtcaat catcacgcgg tctacgtagt catgtccggg
     8821 gagataaagc tggtcagaag gaatagtcat acaacggtgc tgtaaaaggt tgtcggcgtc
     8881 tttgccaagc aactgcgcaa tatctgtcat tactatgctc ccgtaaattc cgattggata
     8941 tcggctatgg attgtcctgg cccgcctttt gcgggcaatc ataatcctgg tcgttacggg
     9001 cgagtttttc catcttttgg atgttttttc agcgttttct gttggctcga ttcatcagaa
     9061 aaaatgttag cgcggtcaag ttttcaccgc aaaggtattt aaaaggtatt attaagtggt
     9121 attgtcatcg cgtaccttac attacctgtc atgaaggaat taaaagatga aaacaacagc
     9181 aaagctgtcg ttcatgatgt ttgttgaatg gtttatctgg ggcgcgtggt ttgtgccatt
     9241 gtggttgtgg ttaagtaaaa gcggttttag tgccggagaa attggctggt cgtatgcctg
     9301 cacagccatt gcggcgatcc tgtcgccaat tctggttggc tccatcactg accgcttttt
     9361 ctcggcgcag aaagtgctgg cggtattgat gttcgctggt gcggtgctga tgtatttcgc
     9421 cgcgcaacag accacttttg ccgggttctt cccgttactg ctggcctact cgctaaccta
     9481 tatgccgacc attgcgctga ctaacagcat cgcttttgcc aacgtgccgg atgttgagcg
     9541 tgatttcccg cgcattcgtg tgatgggcac tatcggctgg attgcctccg gtctggcatg
     9601 tggtttcttg ccgcaaatac tggggtatgc cgatatctca ccgactaaca tcccgctgct
     9661 gattaccgcc ggaagttctg ctctgctcgg tgtgtttgcg tttttcctgc ccgacacgcc
     9721 accaaaaagc accggcaaaa tggacattaa agtcatgctc ggcctggatg cgctgatcct
     9781 gctgcgcgat aaaaacttcc tcgtcttttt cttctgttca ttcctgtttg cgatgccact
     9841 ggcgttctat tacatctttg ccaacggtta tctgaccgaa gttggcatga aaaacgccac
     9901 cggctggatg acgctcggcc agttctctga aatcttcttt atgctggcat tgccgttttt
     9961 cactaaacgc tttggtatca aaaaggtatt attgcttggt ctgatcaccg ctgcgatccg
    10021 ctatggcttc tttatttacg gtagtgcgga tgaatatttc acctacgcgt tactgttcct
    10081 cggtattttg cttcacggcg taagttacga tttttactac gttaccgctt acatctatgt
    10141 cgataaaaaa gcccccgtgc atatgcgtac cgccgcgcag gggctgatca cgctctgctg
    10201 ccagggcttc ggcagtttgc tcggctatcg tcttggcggt gtgatgatgg aaaagatgtt
    10261 cgcttatcag gaaccggtaa acggactgac tttcaactgg tccgggatgt ggacttttgg
    10321 cgcggtgatg attgccatta tcgccgtgct gttcatgatt tttttccgcg aatccgacaa
    10381 cgaaattacg gctatcaagg tcgatgatcg cgatattgcg ttgacacaag gggaagttaa
    10441 atgaaaacag aacgtattct cggtgctctt tatgggcagg cgttagggga tgcgatgggg
    10501 atgccctccg agctttggcc acgcagccgc gttaaagcac actttggctg gattgaccgt
    10561 tttctgcctg gaccaaagga gaataacgcg gcctgttatt ttaaccgcgg cgaatttacc
    10621 gatgacacct cgatggcgct gtgtctggcg gatgcgttac tggaacgtga aggcaatatc
    10681 gacccgaatc tgattgggcg taatattctc gactgggcgc tgcgtttcga cgcctttaac
    10741 aaaaacgtac taggtccgac ctcgaagatt gctctcaacg ccattcgcga cggtaaaccc
    10801 gttgctgaac tggaaaacaa cggcgtgacc aacggcgcag cgatgcgcgt ctcgccatta
    10861 ggttgtttgc ttccggcgca cgatgttgat tcctttattg atgatgtggc gctggcgtcc
    10921 agcccgaccc ataaatccga tctggcagtt gcaggtgcgg tagtcatcgc atgggcgatt
    10981 tctcgtgcca ttgacggaga aagctggtca gcgattgtag attcactgcc ttcaattgcg
    11041 cgacatgcac agcaaaaacg catcactacc ttcagcgcct cactggcagc acgtctggag
    11101 attgcgctga aaattgtgcg caatgccgac ggcactgaat ccgccagcga acagctttac
    11161 caggtcgttg gcgcaggtac cagcactatt gagtccgttc cgtgcgccat tgcgctggtt
    11221 gaactggcac aaaccgaccc gaaccgttgc gccgtcctgt gcgctaacct tggcggcgac
    11281 acagacacca tcggtgctat ggcgacggca atctgcggcg cgttgcatgg cgttaacgct
    11341 atcgatcctg cattaaaggc ggagctggat gcggtaaatc agcttgattt caaccgctat
    11401 gccacagcgc tggcgaaata tcgtcaacaa cgggaggtgg tatgagcggc gctcgattac
    11461 acacgctgct gcctgaatta accacgcgtc agccggtgat ggtcgtcggc gcggcggtca
    11521 ttgacgtgat cgccgacgcc tacgccctcc cctggcgtgg gtgcgatatc gaactgaaac
    11581 agcagagcgt taacgttggc ggctgcgcac tgaatattgc cgtggcgtta aagcgcctcg
    11641 gcatcgaagc gggtaatgcc ttgccgctcg gtcagggcgt gtgggcggag attattcgca
    11701 accggatggc gaaagagggg ttaatcagcc tgatcgataa cgccgaaggc gataacggct
    11761 ggtgtctggc gctggttgag ccggatggcg aacgtacttt tatgtcattc agcggtgtgg
    11821 aaaatcagtg gaatcgccag tggctggcgc gattaaccgt tgcgcctggc agcctgctct
    11881 atttttccgg ttatcaactg gcctcgccct gcggcgaatt gttagtggaa tggctggaag
    11941 agctgcaaga cgtgacgccg tttatcgatt ttggcccacg tattggcgat atcccggatg
    12001 cattactggc gcggatcatg gcctgtcgcc ctttagtgtc gctcaatcgt caagaggctg
    12061 agattgccgc cgaacgtttt gctttatccg cagagataac aacacttggc aaacaatggc
    12121 aggagaaatt tgccgcgccg ttgatcgttc gtctcgataa agaaggcgca tggtatttca
    12181 gcaacgacgc ttctggctgc attccggcat ttccaacgca agtcgtcgac accattgggg
    12241 cgggcgacag tcatgccggt ggcgtacttg ccgggctggc ctctggtctg ccactggcgg
    12301 atgccgtatt actgggcaat gcagtggcgt cgtgggttgt cgggcatcgg ggcggtgatt
    12361 gtgcgccaac gcgcgaggaa ctactcctcg cacacaaaaa cgtatagatc gctgcgacag
    12421 tggctaatgc tgtactcaat aggccgttgc tgttggtcaa gcgccacttg cttgatcacc
    12481 agcaccggta ttttgctatc taactgaata tgtgactgaa actcggcatc tggcatccgg
    12541 gcgctaacgc gggaacgtgt acgttgtggg taaatgtgct ggctgcggaa atagtcataa
    12601 agagaaattc cgatggcatc aacatcatga attaaatgcg ccggaaccca cgattcctca
    12661 atcgataccg cttcttcatc gacatagcga atacgcttaa gcaaaaagac atcacttccc
    12721 gcttcgaccg ccagctgctg cgcgacttct tcgggacatt tcacgacacg cttgttaacc
    12781 catagcgtat cgggcttttt cccgcgcaat accacctgct gagaaaaacc tcgcgcttct
    12841 ttcaacgagt attcgaagat gttgttgatc tgcgtgccgt aaccgcgcga acgcgtcacc
    12901 acgccctctt cttccagcgc ctgcatcgcc ttgcgcaccg taatgcgtga aacgccggtt
    12961 aactgactta aatcacgctc accgggcaaa atattgccat gctccagcac cccgctgcgc
    13021 acggcatttt ttaccgtttc ggcaaactta atataaagcg gcgtgttatc cgccgctaaa
    13081 atacgctcgt taagttgggc aatcagctgg gtatgcgctt gttccatttg tctttttcct
    13141 gccagggagt ctggtgggag tatatcactg cttcttattt aaggcgtttc tttaatccca
    13201 tcaacgaatg cctgtaactc ctccaccgtg ccattaaaca cattaaaatc caccggacca
    13261 ttgatgccat ctacctgtcc acggtcggaa tgctgccaga agcgccaggc catgccgtca
    13321 ttgtccggac gacgttgata gtagtgagcc acccaccacg gatattcatt gaaatagccc
    13381 gccagattgg tgtgataaaa aacggctcct gagtaaataa tcagcttttt tcccgtactt
    13441 ttttcgacca tttttagcca ctgacttacc cgcttgcgta attcttttgc cgataatttt
    13501 ccccgttctt ctacgtccag cacggcaggg aaatcgcctt gtgagaaatc caccgtttgc
    13561 agaaataatc gcgcctgacc aggcgctgct accgacgggg agaaataatg atacgccccg
    13621 cgcagcaggc cattttcgcg gcttagttgc cagttacgcg aaaaataggg gtccaccagc
    13681 ttttcgcctt ccgtagcctt aataaaagca aactgtaagc ggataccgtt gtcgcgcatt
    13741 tttgccaccc gctgccagtc gatccgctcc tgccagcgcg aaacatctat tccgtgaatg
    13801 gtgtaactgg caggaatgcg aatagcgaaa gatttaaccg ggcgataacc gtagaagtgg
    13861 atgtagtctg taatctgaac tgctgtcgaa tagaaaaaat tcaccgtctg acgcggataa
    13921 attgcaacga tggaaattag cacaagagcg cataatagag cggtgagttt ttttcgactg
    13981 gtgattctca gctgcattgg tttcatccct gaatgtcagt gccaaaggct gacaataacc
    14041 aaagcagcta tagtacggtg ctcaccacca ggcgtggaag tggtgaaccg gaccaatacc
    14101 gtgaccaact tccagcgtgt cggcctgggc taatgctgat gaaagccagc tttttgcctc
    14161 ctgtacggtg tcagcccagt ttgtatggcg cgggcgtagt gcagccaacg ccgcagagag
    14221 cgtacaacca gtgccgtggg tgtttttggt cataatgcgc ggtgcggtaa accgttgttc
    14281 accctcgcgg gtaaacagcc agtccgggct ttgctcatca tccagatgac cacctttcat
    14341 tagcactgcg ccacagccca tcgccaacag cgatcgccct tgttccagca tttcctgttc
    14401 ggtgcgcgcg tgtggcgcgt cgagcaaggc agcagcttcg ggcaagtttg gcgttattaa
    14461 tgaaacctgt ggcaataatc gactgcgcaa cgtagcaacc gccgaaggtg aaagcagcgg
    14521 gtcgccgctt tttgccagca taacggtgtc gagtaccacg ttttggatct gataacgttg
    14581 caaccgttct gccaccgctt caacaatatc ggtttccgcc aacataccga ttttagtggt
    14641 atcgattcgc acatcgctga acaccgaatc gagctgggcg gcgacaaaat caggctcaat
    14701 gcgatacacc gactgtacgc cacgggtatt ttgcgccacc agtgcagtaa taactgagca
    14761 accataagcg ccaagtgccg agaaggtttt aagatcggcc tgaatccccg caccaccact
    14821 cggatcagta ccggcaatcg tcagagcgtt aattcgtttc atgcctgcac ctcctgcgtc
    14881 aattgccaga gcgcatcaag gaaatgtgga acaaaactgc ctggcccctc gcttctggcg
    14941 actgcgcgtt ctccggcttg tttcatccag tgacaggcag atgcgacatt ttccagcgta
    15001 tcgcctggta acgcacagca ggcagcgaca accgccgata acgcacagcc agttcctacc
    15061 actttggtca ttaacggatc gccaccgtga ataccaatga tacgatgtcc atcggtaaca
    15121 taatccacct cgccagtgac cacgacgatt gcgccagttt cccgtgccag tgtttgtgca
    15181 gcgggtatcg cattagctgc cgcgtcagtg gtatccactc cccgtccgcc agtagcaacg
    15241 ccagctaatg ccatgatttc cgaagcatta ccacgtatcg ctgccggttt aaaagataaa
    15301 agttcatgac aaaaatggcg acgataatcg agcgcaccaa ccgctactgg atcaagcgtc
    15361 cagggggttt gagagctttt tgcttgctca acggcggcac gcatcgcctg tgcgcgtggc
    15421 tgcgtcagcg tgccaacgtt aatcaacaag gcactggcga tagccgcaaa ctgactggcc
    15481 tcttcggttt cgataaccat cgctggcgat gcaccgatcg ccagcaaggt attggcagta
    15541 aaggtttgca ccacatcgtt ggtcatgcag tgcacaagag gggaatgttg gtgaaaaagg
    15601 tgtaacgtgt gcgcagattg cgcgaaactc agcaggtcga cttgcatagt ttgctcctgc
    15661 cataacgtga agaagcaatg acctggtggt ccgtgacttc cctacgctgg cattatccag
    15721 atcaggtgat acgggtattt cttactaatt tttgttgtca gttgataatt aataagtttt
    15781 tagcacacaa gaattaaaaa cctaaaaact gaccacacaa ctgaccacac atttcgttga
    15841 agctctgcga acaatgacga acaataacaa acaaaaggga aatttgctgg cggttttgct
    15901 taaccgttat cattttccgg ctatccgtta tgaagctggt ggatgttccc cgatatgggg
    15961 atccccacct accggaattt ttttccggtt aaacgttaat caggctggtg ggctttacct
    16021 gttcggtagg tgttatgatt atcgtaatac ctttccccca atggggtaaa agcatcaacc
    16081 gtaacgaaag tcgttatggt tgccactgat accccaaatt tgggtatcac gagtaagacc
    16141 caaaatctgg gtgttactct tactacccaa actacgggta gtttccgtag tttcatggtc
    16201 gagttgcaga tctgcaactc ccccattaac cacaggcgaa tttccgcctt cggtcattcc
    16261 ttaatcatct gcgtaatcat aatgattatt cagcaagcgc aattttgcac tttgcttaaa
    16321 gttcagcgag ttaccgcgcc gacttcctca aattgaggtt tccgaaaata tcagcgggtt
    16381 agcgattaag ctgatcatta attaaactgc gaaaatttcg tagtttgtga ctgtctttca
    16441 ggcagcttgt taacagtcct cagaagcgag ggatgtagcc acccattttt ggtccgtcgg
    16501 gaatatcaac cagttaccgc cgcaaccgct ccggcttctt ccagtggtac gttattttct
    16561 ccttctcccg atacatctcc acgcggcgac ggtaggccag caactcaaga actctggttc
    16621 gtatgttgcg catatccacg ccattaagtt ctataccatc acggcgcatc acctcagcaa
    16681 caacacgcgc atagttttcg gctgtcacgc tgtccggctg cgtggcctgt tcgtcagcct
    16741 gctggctgat tccggcaacg cggcggatta atcccgttat ttcggcttct gtcatgctgc
    16801 tgacctcatt acaccccgct aaattcttcc agtttctggc ggtggctgtc gcttatatca
    16861 aaagcaaaat cctcatgctc tgcctggaat gtaccaaacg ccatcagcgc cgccacgctc
    16921 gggtctatct tgttcggtga ttttttcttg ttcggcttga tattggcgtt cgcgtcactc
    16981 tgcatcacga cgttactcat cgaccaggac aacaccggat cgccacgatg cacaatcacc
    17041 ctgcggttaa caaaaacttc gaacgatttc gccgccggac tgaatctgag gtaggtttgc
    17101 gggaacggct ccacctcaaa acctgccccc tgtaattgcg ttctgagatg cgtggcgttc
    17161 cacgtatcaa agcccaccag cctgatatta aatttctccg cgtcctgcat gatgtcatct
    17221 ctgatccggt cgtaatcaat gcagtcgcca ggcgttgtgc gtatccagcc tgctttagcc
    17281 cactgacgat agacagcgcg gtttttattg gcggggttct gtagctggaa ctccggcaga
    17341 taatgacggg aaatcatcat aatggtttta ccgaccggaa aggcatagca cacgctggaa
    17401 atatcgctgg ttgatgataa gtccagcccc gcgtaacact cctgaccgtg taaatcttcc
    17461 tccgtgaacg ttccggcaca ctcagcccat gcaccattac ccatccacgg ggtagtcccc
    17521 tgacaccaga tattaaatcg ctttgtcatc atctccaccc attgcgacgg aataccccgc
    17581 gctttctgaa tggttgaggc cagtttttca cgatccacgg aaacatcgat attagggttc
    17641 gcctttatcc acatctccgg atcatcaacc tcgttttcgt cgtccagttc gtaaatcagc
    17701 acaaaaagcg attcgttaac ctcttcgccg tccaggatct ggcagcaata atcataatgc
    17761 tgcttacagg ctgaaacgac gttacttccc gatgtggtaa tggcaaataa cagcccttcg
    17821 ggacgtgccc ccatccccag ttcaagcgca gaatatacgc cgttgtcggg gtgtaggtgg
    17881 tattcgtcca cgatagaaag actggggttt gtgccctcga tagttgccgc ttttgctgcc
    17941 agcggcttta acaggctgtt ggttttcggg tgtatcacct tatgcgcctg aatattcacc
    18001 cgcctgcgta acggtcggga taaaatgcac atctgacgcg catcatcaaa tacgatcctc
    18061 gcctggtcac ggctcaccgc tgcggtgtaa atatcctgct gaccgttctc cataatcaga
    18121 aaccagttag ccagaatcgc ggcggtcgtt gatttagcat ttttgcgcgg cacttcgata
    18181 aaggcgctcg tgtatttgcg ccgtccggtg gccttaacct taaagccgag gatgcacgca
    18241 aaggcgaact gctgccacgg ctccagctca atgggtctgc cacgcatcgg cccttttacg
    18301 tgcgggcaca ccctggaaaa ggcaataaac cgctccacaa cctcacgatc gaacgtataa
    18361 agggggtttt taaggtccga aaagtaccgt ttaacggcct gtttcagccg tttacaggcc
    18421 ggaattttgc ccgtttttac gtcttctgcg tacttattcc aggcggtcaa gctcgtcctc
    18481 ttcttctgtt tccggtggat ttttacggcg gcttatcggg tcaaaaccga gcaaggaggc
    18541 catttttatc atcactcttt cagcgtcgga ttttgcgctt aatgcggggt ttctgctctc
    18601 gccgccctga ctgttaacaa tgctgaaccc gcgcgccgca aggtctgcaa cggctttccg
    18661 gtaaatggag tagttgacac aatacagttc cagattgctc cagtcggcgg gggtcaggtc
    18721 tccccgttcc gcaagctgcc gcgatttttc ccgccactgc ttcacggcga tgtcatccag
    18781 gtaggcgggg actttcggtg gtcttgccat gcttattttt cgccagatta tttttcaaaa
    18841 aattcccgtg cataaaaatt tgaggaggcg ttcggttctg ggcggggtcg ggttcgtcct
    18901 gaaaactccc cccaccccgt ccggctgcct catcagcgat tgcggaaaca ttccataacc
    18961 tcgcggtcac ggtcgtttaa tcgcgtcgct gtgatgcgtt ctgcgcgtcc tgatgcttta
    19021 tctttatgcc ctgtttcctg tgccttccat gcgtcacgct gccttataag tccacggata
    19081 aggcggtttt gttcccgctc attcatcagc gccatacatc cagttattgc ggttagcggc
    19141 ccgttcttcc tcctcacgga atccacccgc agcacgcttg ttttttgtgg acggatcaag
    19201 ccatttcgtt ttctggttat gacacgcctg gcacaatggc tgatgattcc actcaggcca
    19261 gaagagaaca tcattaccgc catcgattgg gataatgtga tccaccacca cggcgggcgt
    19321 gtatattcct ttttccaggc aatgcgcaca taacgggttt ttactcagat acatggcgcg
    19381 gtatttctcc cactgcctcg tataccctcg ctccctgctg ctgcctctcc ggctgtcctg
    19441 ctgctggcgt gcgctgcgtc tgtgctcctc acacttgccg gacttcacgc gcctgttgca
    19501 gcctggttct gtacatcgtc ttaatggttg ccacggcatc agtacacccc cacatcacgg
    19561 taagccgtcc agagtgcgcc aatcgtcatg ggtacgtgtg tttttgcgtt atccgcgaca
    19621 atctggcgat tctcatacag gtgagcgata aacatcatgc agccaatctt tatggctggc
    19681 gtgaactcca gcccgtcatc aaagcgcctg cctatgtgta tctggcacgc ctcaagcgat
    19741 gcggcaatgt atccgctgat taactggtct tcctcgtcgc catcgatgcg gcaatggagt
    19801 ttcacttctt ccagggtgat aagttcttct gtcatttttc cgcgccctca cgacaaagaa
    19861 tttcaaggcg tgtcctggcg gcatccggca gcggctgccc gatgatattc agtacacgcc
    19921 ccgccagcgg ccccgtattg acctttatcc ggctggtggc gttgatgtcc ttccggtacc
    19981 gtatccagat ccttacggta ccaatcgcca gctctgcacc cgatgaaatg gcctccttgc
    20041 tgctgaccat gttcacgctt gcccagagtg tgtgaccgtc ctcccacgtt tcgagtattt
    20101 cgcccgtcat ggctctggtc tgtttcaggg tctgaatcgt taccctgtca cgcagtcgcc
    20161 ctatgttcat tccggttttt ctccctcgct gatttttact tcctgtttcc atgcctggct
    20221 gaactcatca ccaccatcac gcggagacaa cccttctcgt tcgcgggctt cgttcgggca
    20281 cataacaccg gatttgatgc ctcgctcata ggtggcaaag cgttcgccag gtgtggcccg
    20341 taacaggtcc gcgctgtcaa actccacctg ataccggata ccaggtacag gcgagaccac
    20401 cagcaaggcg ttttttatct gctgctcaaa gttcgccagc cacgggcgca tcgtcatggt
    20461 gagaaatgcg cggcttgcct cgctgaaatt gctgtaggtg ctgttgctgt attcctgcag
    20521 aaaaatcggc gagacgttga acattcgggc gatgtcttca atggtgaagc gacgggaggc
    20581 cagccattcg gcgtcctggt tactcatgcc cagctgctgg taactcatgc ccccttcaag
    20641 gatgggcgtt tttccggcgt ttctggcccc tttgtagcgt tccagtgcgg ctaatgcctg
    20701 tttgcctttc acgccgtcca gccattcgcc tgacgtgata acccctgccg ccatcatgcc
    20761 atcacgcatc acgctcgcgc cgtggcgttg ttgggccagc ccaagcccca gcgattcgcg
    20821 gcagatggtt acaggtgagc gccccataaa gccgtcatcc gtggagtaac gaaggtgaag
    20881 tacttcccac ggtaaatagt tgcgggtgtt tccggtgtag gcgtcagtga tgcaatagcg
    20941 ccagttgtgt tctcctgtct gctccacgtt caccgactgc ggcgggtaag gatgtaaagc
    21001 cgccggaaaa ccatcacgcc cccactgaat caccgcgtaa gcattaccgt ttaacaggca
    21061 gtggcggatc atcattcgct taaactggta gggggtttgc catgcgttcg ggcgctcgtt
    21121 gaggatatga tcgaccggat gagaatcaag ccactcgcgg gcctccttac ccttctcatt
    21181 gcgtaccagg tacaggtaac acggcatggt agccaccgcc tcagagatga ccgtcacggc
    21241 gttcatgacg gcgggcagtg attccgctgt ccccgatgat acgtactcac ccgcccccgt
    21301 gttcgatgtg ccagccagcg ccataaattc atcaagcgtc atgctgcgct gctctttttt
    21361 tcttctgaaa agccacatat cacacccccg ctaaatccac ccaccagcgg cgattatccg
    21421 cacgcggcat tttttcgggg tgctgctcat acagggaacg gcgggccagc tccacgccgg
    21481 aatcggggta agccggtacc gatgtaacgg taatttcgta cagttccgcc accagcacgg
    21541 tgcgcacgca tggatctgtt gtggtatccc atacatcctt acgggagcga aagccaaagc
    21601 tcatccccga gatgtcccca cgcttaacca gttcgataac gtcgcgccct gtactggtat
    21661 ccggtggggt cagttcaaag cgtaaccctg tttcgtcctc ttccagtttc agcgtgccgg
    21721 aacgggtacg ccccagcaac atgctgtggt catgctcata caggccgcga acgtcattac
    21781 ccgccgcaag ccattcagta aacgctcccc gctggaattt ttcgtaaaac tccccccata
    21841 gcagctctga aagcttatcc cagcgaacaa cgtagcctgt aagcgtgttg ctggcgctgg
    21901 tggtgatttc cgatgaccgg atttccatac tcttcataat tttttcaccc ataaaacact
    21961 aaaggggctt ttaagcccct tctgtatgct gttaatcgtc gtcctgtggc agttccagaa
    22021 tcttgatcgc gttcgaatcc accacgccac cgcctaaata tttctgcgtg aaaattttga
    22081 tgaagcccgg ctctgtgagg ttgtccggtc tggtgcgaac acctgtttcg tgatcaacaa
    22141 tgtagtaacc gcgtttgaag tcacccaggg caataacgtt atcaggcata aactccagat
    22201 attcgaccgg aaggcccagc aacgtatcag gatcaccagc ctgtaaacgg tcgcgccaga
    22261 tgtaatcacc gttcgcgttc ttcaccttct gaagttttgc cgccgtcgtg gagttaacca
    22321 cccagaccgc gtttttgcgg tattttttac gtaatgcaaa tttcaggtcg atcagcgggt
    22381 ccgcagatgt ccacgccaga gattcggaag gtttaattac ctgcaacgta ccaaaatcac
    22441 gctctttgtc gttcttctct gcacggggta cggataaaaa accttttgct tttttgtcac
    22501 cgtcgcccac aaccagatcg ctttcttcgg tttccgtgaa ggtgtcgcca atctcacccg
    22561 tcagccatga aaggatgtcc acatcggaaa aatccacgat ttcctgtgtg gtgcgcgggt
    22621 acgcatagac cggatacagc ttaatgctca cctcgttaat ctgcggggtg ctggtctgtt
    22681 cgcgtgtctt accctcttca ccgtggttaa cggtcgcacc gccagcggaa acaagctgct
    22741 taaactcgtt gctgctgatt ttcttcacgg tacagatgcg gcgcatggtg gattcatccg
    22801 tcagcattcg catgatttcg gtgttcagtt cggggataac ggtataacca ccatcagcgg
    22861 gaacgcctgt acttaatgcg cgggtttcac ctgtcaggat gtagttgcgg agttctgccg
    22921 ggtcagtggt ctggctgctt ttacctggct tgctgcgctc ttcgtctgca atggcttcaa
    22981 ggcgggaaat gtctttatcg agggattcag ctttagcgcg taattcgtca aattttgcgc
    23041 cctctgcatc gttaaggctg cggttttctt tttccgcgtt ctccagcatg tcgcgcatct
    23101 gatttttaat ggcggttttc tgctggcgta attcgattat tctcggcata aaaaaaagtc
    23161 ctggggttaa gtaaggaact ccaggacgcg gcaaaaactc aaccgttttt cataaggaaa
    23221 tcagcaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc acattaacag
    23281 gcgggaaagt ggccccagcg tcctggcacc acgggcgaga atactcatga ttcagttcgg
    23341 gtaaaatatg ccgatcctgt cagtgaacaa cgtggaacaa ccacgaacaa ataatttaca
    23401 aaaaatgaca aaaagccgga ttgctccggc tgtatgcgtg ctcagtccct gatccctttc
    23461 agaaattcta tcaatgcatc tatctgttca ggatttaccg ccagcatttc accggacaga
    23521 gcacatctca caaaaccatg ctgatccttt tcaatcagtg cgcccgtctc caggaatgcg
    23581 cggtaatcat tgatgctcat cgtctccatg ttgtcagcat ggtatttatc acgctgtttt
    23641 aatatctcat caa
//
LOCUS       DAZFKF010000075        23478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFKF010000075 DAZFKF010000000
VERSION     DAZFKF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.075"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1348
                     /gene="paoC"
                     /locus_tag="ACHKA1_003491"
     CDS             <1..1348
                     /gene="paoC"
                     /locus_tag="ACHKA1_003491"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO5681094.1"
                     /translation="TLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYA
                     GANRHTGLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQ
                     VDPADPTRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLL
                     EKSGARVHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPV
                     SAGSGGQWGANTSTSGVYAACMKLREMIASTVGFDPEQSQFADGKITNGTRSATLHEA
                     TAGGRLTAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILN
                     PKTARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLD
                     DTDPISSPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            1358..2314
                     /gene="paoD"
                     /locus_tag="ACHKA1_003492"
     CDS             1358..2314
                     /gene="paoD"
                     /locus_tag="ACHKA1_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO5681095.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGCEREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLADVASVRLHQEEDSYLPPSS"
     gene            complement(2492..3619)
                     /locus_tag="ACHKA1_003493"
     CDS             complement(2492..3619)
                     /locus_tag="ACHKA1_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5681096.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPITRDLGVTEGLAGQGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPMA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSTFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWTVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(3761..4819)
                     /locus_tag="ACHKA1_003494"
     CDS             complement(3761..4819)
                     /locus_tag="ACHKA1_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5681097.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDVAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGKDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLRYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            5084..5968
                     /locus_tag="ACHKA1_003495"
     CDS             5084..5968
                     /locus_tag="ACHKA1_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5681098.1"
                     /translation="MAKRENYNELYLFMQVVREGSFTAAAQRLGLAQSGVSRSVRELE
                     ERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGTVRINAS
                     QHAIDKCLLPKLAVFKQRYPDIRLELINESRFVDIIEERFDAGVRLGPEVGQGMVAVR
                     ITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHR
                     PEGQWALSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRMEGLFLYY
                     PHRNVSPALRMVIDTLKI"
     gene            6211..6267
                     /locus_tag="ACHKA1_003496"
     CDS             6211..6267
                     /locus_tag="ACHKA1_003496"
                     /inference="COORDINATES: protein motif:HMM:NF041474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681099.1"
                     /translation="MVVFVATVEYAYLSLHFY"
     gene            complement(6671..6949)
                     /locus_tag="ACHKA1_003497"
     CDS             complement(6671..6949)
                     /locus_tag="ACHKA1_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681100.1"
                     /translation="MPMYIKLILSIVRRISVNTICSLIVVVTLSLLSFSSVAKTITAV
                     GSTIDSTEKEISLQAEKQGKSYKILGAFFKNRVYMIAKLTPVSKNDAS"
     gene            complement(7115..7381)
                     /locus_tag="ACHKA1_003498"
                     /pseudo
     CDS             complement(7115..7381)
                     /locus_tag="ACHKA1_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            7387..7794
                     /locus_tag="ACHKA1_003499"
     CDS             7387..7794
                     /locus_tag="ACHKA1_003499"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5681101.1"
                     /translation="MVVASPSYLAANSTPVHPRELVNHKCIGWRQSPDVAPYRWPFEE
                     NRRAFDLAIEPQITTNDLRLMLRLALAGGGITIATQETFRPYIESGRLVSLLDDFLPQ
                     FPGFYLYFPQRRNIAPKLRALIDHVKEWRQQLA"
     gene            complement(7921..7996)
                     /locus_tag="ACHKA1_003500"
     tRNA            complement(7921..7996)
                     /locus_tag="ACHKA1_003500"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7961..7963),aa:Thr,seq:cgt)
     gene            complement(8111..9364)
                     /gene="proA"
                     /locus_tag="ACHKA1_003501"
     CDS             complement(8111..9364)
                     /gene="proA"
                     /locus_tag="ACHKA1_003501"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO5681102.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLDKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCSLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRV"
     gene            complement(9376..10479)
                     /gene="proB"
                     /locus_tag="ACHKA1_003502"
     CDS             complement(9376..10479)
                     /gene="proB"
                     /locus_tag="ACHKA1_003502"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO5681103.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            10767..11822
                     /gene="phoE"
                     /locus_tag="ACHKA1_003503"
     CDS             10767..11822
                     /gene="phoE"
                     /locus_tag="ACHKA1_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO5681104.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(11861..12262)
                     /gene="crl"
                     /locus_tag="ACHKA1_003504"
     CDS             complement(11861..12262)
                     /gene="crl"
                     /locus_tag="ACHKA1_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO5681105.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(12320..13564)
                     /gene="frsA"
                     /locus_tag="ACHKA1_003505"
     CDS             complement(12320..13564)
                     /gene="frsA"
                     /locus_tag="ACHKA1_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO5681106.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(13656..14114)
                     /gene="gpt"
                     /locus_tag="ACHKA1_003506"
     CDS             complement(13656..14114)
                     /gene="gpt"
                     /locus_tag="ACHKA1_003506"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO5681107.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            14375..15832
                     /gene="pepD"
                     /locus_tag="ACHKA1_003507"
     CDS             14375..15832
                     /gene="pepD"
                     /locus_tag="ACHKA1_003507"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO5681108.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREEVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(15889..16197)
                     /locus_tag="ACHKA1_003508"
                     /pseudo
     CDS             complement(15889..16197)
                     /locus_tag="ACHKA1_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
     gene            complement(16217..16425)
                     /locus_tag="ACHKA1_003509"
                     /pseudo
     CDS             complement(16217..16425)
                     /locus_tag="ACHKA1_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(16616..17068)
                     /gene="yafP"
                     /locus_tag="ACHKA1_003510"
     CDS             complement(16616..17068)
                     /gene="yafP"
                     /locus_tag="ACHKA1_003510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5681109.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
     gene            complement(17078..17476)
                     /gene="yafO"
                     /locus_tag="ACHKA1_003511"
     CDS             complement(17078..17476)
                     /gene="yafO"
                     /locus_tag="ACHKA1_003511"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFO5681110.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(17479..17772)
                     /gene="yafN"
                     /locus_tag="ACHKA1_003512"
     CDS             complement(17479..17772)
                     /gene="yafN"
                     /locus_tag="ACHKA1_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFO5681111.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            complement(17824..18879)
                     /gene="dinB"
                     /locus_tag="ACHKA1_003513"
     CDS             complement(17824..18879)
                     /gene="dinB"
                     /locus_tag="ACHKA1_003513"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO5681112.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(18950..19735)
                     /gene="lafU"
                     /locus_tag="ACHKA1_003514"
     CDS             complement(18950..19735)
                     /gene="lafU"
                     /locus_tag="ACHKA1_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO5681113.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            19707..21419
                     /locus_tag="ACHKA1_003515"
                     /pseudo
     CDS             19707..21419
                     /locus_tag="ACHKA1_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(21612..22109)
                     /gene="rayT"
                     /locus_tag="ACHKA1_003516"
     CDS             complement(21612..22109)
                     /gene="rayT"
                     /locus_tag="ACHKA1_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFO5681114.1"
                     /translation="MSEYRRYYLKGGSWFFTVNLRNRRSQLLTTQYQTLRNAIIKVKR
                     ARPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHT
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGNITDFSAG
                     ERIIS"
     gene            complement(22285..23034)
                     /gene="yafL"
                     /locus_tag="ACHKA1_003517"
     CDS             complement(22285..23034)
                     /gene="yafL"
                     /locus_tag="ACHKA1_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5681115.1"
                     /translation="MSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            23244..>23478
                     /gene="dinJ"
                     /locus_tag="ACHKA1_003518"
     CDS             23244..>23478
                     /gene="dinJ"
                     /locus_tag="ACHKA1_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO5681116.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVGVHKAKDAD"
BASE COUNT         5754 a         6132 c         5989 g         5603 t
ORIGIN      
        1 cacccttcag cacttgcgta tcggtgccga ccagagcggg aaaatcaccg ctatctcaca
       61 tgaaagctgg tccggaaacc tgcccggcgg cacgccggaa acggcggtac agcaaagcga
      121 attactctac gccggggcaa accgtcatac cggcctgcgg ctcgccacgc ttgatttgcc
      181 ggaagggaac gccatgcgtg cgcccggcga agcccccggt ctgatggcgc tcgaaatcgc
      241 gatcgacgaa ctggcggaaa aagcgggcat cgatcccgtc gagtttcgca tcctgaatga
      301 cactcaggtt gaccccgccg acccgacgcg ccgcttctct cgccgtcagc ttatcgagtg
      361 cttgcgcacc ggagcggata aatttggctg gaagcagcgc aacgccacac ccggacaggt
      421 gcgcgacggg gagtggctgg tcggccacgg tgttgcggcg ggctttcgca ataatctgct
      481 ggaaaaatcg ggtgctcggg ttcacctcga acaaaacggc accgttaccg tagaaacgga
      541 catgaccgac attggcaccg gcagctacac cattctggcc cagacggcag cggaaatgct
      601 tggcgtaccg ctggagcagg ttgcggttca cctcggcgat tccagtttcc cggtttctgc
      661 gggttctggt ggacaatggg gcgcgaatac ctccacctcc ggcgtttacg ccgcctgtat
      721 gaagcttcgc gaaatgattg cctcgacagt cgggtttgat cctgagcagt cgcagtttgc
      781 cgacggcaag attaccaacg gtacccgaag cgccacgcta catgaggcca ccgcaggcgg
      841 cagactgaca gcggaagaga gcattgaatt cggaacactg agcaaggagt accagcagtc
      901 gacctttgcc gggcattttg tggaggtcgg cgtgcatagc gcgacgggag aagttcgggt
      961 ccggcgtatg ctcgctgtgt gtgctgcagg acgcatcctg aatccgaaaa ctgcacgcag
     1021 ccaggtcatt ggcgcaatga ctatgggcat gggcgcggca ctgatggagg agctggcggt
     1081 ggatgaccgt ttgggctact tcgttaatca cgatatggcg gggtatgagg tgccggtcca
     1141 tgcggatatc ccaaaacagg aggtgatttt cctggatgat accgacccca tatcctcccc
     1201 gatgaaggcc aaaggtgtcg gtgagctggg cctgtgcggc gtgagcgcgg ctatcgccaa
     1261 cgcggtgtat aacgccaccg gtattcgggt acgggattat cccatcactc tggataagct
     1321 gctcgataag ctgccggatg tggtttaagg aggaacaatg tcatacccgc tttttgacaa
     1381 agacgaacac tggcataagc cagagcaggc gtttctcacc gatgaccacc ggaccattct
     1441 gcgcttcgcc gtagaggcgc taatgtccgg taaaggagcg gtgctggtga cgctggtgga
     1501 gatacgcggc ggcgcggcgc gcccgctcgg ggcgcagatg gtggtgcgcg aagatggtcg
     1561 ttactgcggt tttgtctctg gcggctgcgt ggaggccgct gccgcttttg aagcgctgga
     1621 gatgatgggc tcaggctgcg aacgcgaaat tcgctatggc gaaggttcgc cgtggtttga
     1681 catcgttctg ccctgcggcg gtgggatcac gctgacgctc cataaactac gctcggcaca
     1741 gcctctgctc gccgtgctga accgcctgga acagagaaaa ccggcggggc tgcgctacga
     1801 tccgcaagca caatcgttgg tgtgcctgcc cacgcaaacc cgaacgggct ggaatctcaa
     1861 tggctttgag gtggggttca ggccatgcgt caggctgatg atttacggac gttctcttga
     1921 ggcgcaggca accgcgagtc ttgcagcagc cacaggctat gacagccata tcttcgatct
     1981 ttttccggcc tcagccagcg ctcagatcga taccgatacg gcggtcattt tgctgtgcca
     2041 tgatctcaac cgagagctgc cagtgttgca ggccgcgcga gaggcaaaac ccttttatct
     2101 cggcgcattg ggcagctatc gaacccacac gttacgtctg caaaagctcc acgagctggg
     2161 atggtccagg gaggagacag cgcaaatccg ggcacccgtc gggatatttc ccaaagcccg
     2221 ggatgcgcat actctggcac tctccgtgct ggcagacgtc gcctctgtac gtctccatca
     2281 ggaggaggat tcatacctgc ccccgtcgtc ctgatccttg cggccgggcg tgggaagcgc
     2341 tttctcacct ccggggaaat accataaatt aagagaaaaa tctgcagtag ccgttttaga
     2401 aaatcaccta aatcgtttaa aagagcgccc tacccatagt gggtgctcat cgccttgtgt
     2461 aacttattca gcaacgcgcg ccctcgccgg actaaagcgc accggccttc tggcgtgcgg
     2521 taacaaaggt cagtacgccc gcgcaaagga gcagcacact actcatggca aacgtactct
     2581 gccagccaag gcggtcaaac acgatgcctc ctgccgttga tccaagggcg attgagagct
     2641 gaatcaccgc gaccatgaga ccgcctccgg cttcggcgtt gtcaggcaac gtacgcgcaa
     2701 tccatgtcca ccatccggtt ggcgcggcgg tggccagcat gccccagagt cctaacagca
     2761 gcgagaccgt ccagacgtga tgcccggtaa gcatcagacc gatggcaatg acggccatca
     2821 gtccgggtat ggccatcagc gtggggtaga attttctgtt gagaaatgtc gaaacaacca
     2881 gcgtgccaat aaaaccggcc acgccgataa tcagcaaaat caacgacaag cctaaggaat
     2941 taacccgtgt caccgactcc agaaacgggc gcacataggt gaatagcgca aactgcccca
     3001 tgaaaaacag gccacaagct atcatgccga ccacaaccat gcggcggcta aataagcgga
     3061 ataccgcgcc acatgacgtg tggcttttat tcgcatccat gctgggcaga ctaatgcact
     3121 gccagataaa ggcgacaacc gccatgggca ccaggcacaa gaaagcgcct cgccatccga
     3181 ccgtagcccc cagatagctt cccagcggcg cggccacgac cgtcgccagc gcattaccgg
     3241 cgttgaaaat cgccagcgca cgcgtgacct gatgctgcgg caccagacga atcgccgttg
     3301 ccgctgacat agaccagaat ccgccaatgg ccacaccaat catcgcacga ccgaccatat
     3361 acatcagata actggtagcc agcgcaataa taagccccga tacggccatc agaaccgtca
     3421 tccccagcag caggaactta cgattcatct ttccagctag cgttgaaagc gtcaggctgg
     3481 tcagaacagc cagcgcgccg gagatagcaa tcccctgccc tgcaagcccc tccgtcacgc
     3541 ctaaatcgcg ggtaatcggc gtgagcaggc tgacgggcat aaattcagaa gcaatcagca
     3601 caaacacgca cagggtcata gcaaaaatac cgccccagta tgcgtgctga tgatgaggtg
     3661 atggtgtttg gcttagtgtc gacatagtga aatttcacaa agatcaggat aaaagccacc
     3721 tgccatagac cggtggcttt gcatcaattc agaagaggtt ttatttcagt ttggtctgga
     3781 aaaattgttc gaacttcgcg aaaggtattt ttccggccac gttatcgtac aggtccacgt
     3841 gatttgcccc tggtaccacc accaactctt tgtctttact gccaaccgcc ttgtaagcat
     3901 cttcagcaaa atagcgtgaa tgcgctttct caccggtcac aatcagcgtc gggatggtga
     3961 tttcactggc gtatctcaac agcggcatat tcataaatga cagcggcatc gttgccgtcc
     4021 acgccccggt agagttaacg gaacgttcgt ggaagccgcg cggcatgcga tagtagtcaa
     4081 agaactcttt cagtaccgga tgaggatttg ccggcagcgt ttctggcaga atgcgctcgc
     4141 ccgcactgac cttgccgttg ctgtcgacat taatatcatg accaccgtga gcaaacgttc
     4201 cgctctgcgc atccttccag cgctgttcat tcagatattg cagaacagca cgacggtcgg
     4261 cggtagaata acggtctttg ccatccccca caccatgacc catcgcccgg ctcatgtcat
     4321 acatcacgct ggtagctacc gctttaacgc gggtatccat cgccacgtcg ttcagcgcca
     4381 tgccgcccca gccacaaata ccgagcagcc cgatacggtt gcggtctacc tctttttgca
     4441 gccctaagaa atccaccgcc gcgctgaaat cttcagtgtt gatatccggt gaggcgacgt
     4501 ttcgcggata gccgccgctt tccccggtgt aagaaggatc gaacgccagg gtaacaaacc
     4561 ctcgttccgc cagcgtctgc gcatacaggc cgctggattg ctctttcacc gcgccaaatg
     4621 gcccactgac cgcaatcgcc gccagcttgc gatcgccacg gtctttaggc aggtaaagat
     4681 cgcccactaa ggtgatgcca tatcggttcg ggaacgagac tttacggtga tcgactttct
     4741 ggctctcggc gaatgtttta tcccatttat ctgtcatgga cacaggggca ttaggattgg
     4801 ttgaatcagc ataactcatt gtcgtgactc cacttaatga tgcgaaaagc agcatggtcg
     4861 gcatcgcggt ttttagcgtt cgggtgaagg ctttcataaa aatccccata aaaaacaaca
     4921 gagagataca ggtttccgac ggacaacgct gcggtgcttt actttgatgg ggtcattata
     4981 gtgagcagaa ttagtgctga ttagggggcg attaatgcta agattaatac catattgtta
     5041 taaattaatg cggagattgc cggcatgaag gggggatgtt tcaatggcga aaagggagaa
     5101 ctacaacgag ctgtacctgt ttatgcaggt ggtgcgggaa ggcagcttta ccgcagcggc
     5161 tcagcggctg ggtcttgctc agtcaggagt cagccgttct gttcgcgagc ttgaagaaag
     5221 gctgggtgtc cagctgctag tgcgcaccac gcgtaaactg tcgctgacac aagcaggcga
     5281 gcagctctac cagaagacgg catctggatt tgaaatgtta gatcttgggc ttgccacact
     5341 ggcgcactat cgagagacgc cctccggcac ggtgcgtatc aatgccagcc agcacgcgat
     5401 tgataaatgc ctgctgccaa agctggcggt atttaaacag cgatatcctg atatccggct
     5461 tgaactgata aatgagagcc ggttcgtcga tatcatcgag gaaagattcg atgcaggtgt
     5521 ccgcctgggg cctgaagtcg gccagggtat ggtcgcggta cgcatcacgc ccgatatgga
     5581 gatggcaatt gtggggactc cggaacattt tcgtcgctat ggttttccgc aaactccggc
     5641 ggatttaaag gcgcatccct gtatcgccta tcagtttgcc gacggcagcg tataccagtg
     5701 ggaactcaat caggatgata aaaaaatcac tcatcgccct gaaggacaat gggccctatc
     5761 cgacagctat atggaagcag aagccgcccg gctgggcctc ggattggcct atgttcctgt
     5821 tgaactggtc gcggatgatt tagaacacgg taagcttatc agggtgttgc agcgttacag
     5881 cctgcgaatg gagggattgt ttctctatta ccctcatcgc aacgtctccc ccgccctgcg
     5941 gatggtcatt gatacattga aaatctgaac gttaataacg accgactctt cactgtgcaa
     6001 aattgtgtca ggtagtccct ctgcgttcaa ttccagggtc tgctttgagc gagaaagatc
     6061 agacttatct gtacaagggg actatccagc aatggagatc atccaaaaga aaaaaccacc
     6121 tggctcccct cgacaacctt aatccgggca tttagcctca atcaacattt tcatatctgt
     6181 caggttactt cattatgaaa accgatcaat atcgttgttt ttgttgcaac agtagaatac
     6241 gcgtatttat ccctccattt ctattaataa cggcaataaa ctgttcattt cactgatatt
     6301 taaaatatgc atccttttca agctgttcag cggcataaga aaacgtatta aatatgaatt
     6361 cgacgattta accatttctg tctgattggt aatcatctgt gattaatgct ggtctattga
     6421 ttacgccagc attaaccgct tagggatgta ttcttcctgt tgatatacag ccctcgaaaa
     6481 agagagaaaa taaaactgtg tccaatatta ctctaccact ccagaccgaa gcatcggtag
     6541 aattagcaga acgactggta caatagattg ggatagcgtc atttgtcagg tattccattg
     6601 cctgacaaat gacaattttg agtcaataga cgtgcttctt gatcatgtct taaaaattat
     6661 accatgagcc ttatgaagca tcatttttac tgactggtgt taattttgct atcatataaa
     6721 ctctgttctt aaaaaacgcg cccagaattt tatatgattt cccttgtttt tctgcctgta
     6781 aagaaatttc tttttcagtg ctgtcaatgg ttgaaccgac ggcagtaatc gttttggcga
     6841 cgctactgaa tgataataaa gacagtgtaa caacaacaat taaagaacag atagtattca
     6901 cgctaattct cctgacaatt gagaggatga gttttatata cataggtatt aaacatgagt
     6961 catatttacc ccaagagaaa atactccttc tgtaattaaa ccttcatctc cttcggggtg
     7021 agcaatacat aatgcatctt gctatgaaaa acacagggat atataaatct tccttctgac
     7081 ctatttaata aaacatgaat gtaaaagtta ttcttcagtt tgcagatgca gtgggtctga
     7141 tggtaatttc ggttgtatca acgctttcgg gagattcaat aatatgcctt accgcacggg
     7201 caatatccgc aggttgaagt gcaacagaac ggtaggcgtc cattgccttc atggtttcca
     7261 catgggtaat ggttgaagcc agttcacttt cgacaacgcc aggatttacg caggtcacac
     7321 ggattttcga gctttcctgc cgtaagccat cagaaatagc ccgtactgca aatcaggcag
     7381 cgtgaaatgg tcgttgcctc tccctcctat ctcgcagcca acagcacacc tgttcatccg
     7441 cgtgaacttg tgaatcataa gtgtatcggc tggcgtcaat ccccggatgt tgcgccttat
     7501 cgctggccat ttgaagaaaa caggagagct ttcgaccttg cgattgaacc gcagattacg
     7561 actaatgatc tgcgtctgat gttgaggctg gctcttgccg gcggaggaat aacaattgcc
     7621 actcaggaaa ctttcaggcc atatattgaa agcggtaggc ttgtatctct gcttgatgac
     7681 tttcttccac aatttccggg cttctatctg tatttcccac agcgtcgcaa tattgcacca
     7741 aagctccgcg ccctgattga ccacgtcaag gaatggcggc agcaattggc ttaaatgtct
     7801 gcacctgcat tgcctgatgt cagaacagta ttttgattaa ttgccagggt tacaatggca
     7861 taaatacggc acaggaggaa acgtggtgta tttgaatatg gggtaactac ttgatttaaa
     7921 tggtgccgat aataggagtc gaacctacga ccttcgcatt acgaatgcgc tgctctacca
     7981 actgagctat atcggccctg aaaggacatg ttcacgaacg tgaatcacgg tggacaaggt
     8041 taaaactaac cgggcgatgc gtcaatggcc ttgtgaatca aatggctact tttgcatcac
     8101 ccggttttat ttacacacga atggtgtaat caccaatgcc gatccacttg taagtggtca
     8161 gtgcttccag ccccattggg ccacgcgcgt ggagtttttg tgtgcttacc gccacttccg
     8221 cacccagtcc aaactggccg ccgtcggtaa aacgcgtaga ggcgttaacg taaacagcgg
     8281 acgaatccac ttcgttaaca aaacgctggg cgttgcgcat atcgcgggtc aggatcgcat
     8341 cggagtgttg tgtgccgtgt tcacgaatat gggcgatggc atcgtcaaga tcgctgacga
     8401 ttttgacgtt caaatctaat gacagaaact catcgtcata ctcttcggct ttaaccgcca
     8461 ccaccttcgc ggggcctgcc tgcaactgtg tcagcgcagc tgcatctgcg tgtaatgtca
     8521 cgccgctttc cgccatttgt ttgcttaatg cgggcaggaa gctatcggcg atgtttttat
     8581 tcaccagcaa cgtttctacc gtattacatg tgctcggacg ctgagttttc gcgttgacga
     8641 tcacttttaa tgcttcagcg atctctgcac tttcatcaac gtaaatatgg catacgccta
     8701 taccacctgt gatcaccggg atcgtcgact gctcgcggca cagtttatgc aaaccagccc
     8761 cgccgcgcgg gatcagcatg tcgatgtatt tatccatacg cagcatttca ctgaccagcg
     8821 cacggtcagg attatcaatc gcctgcacgg caccagccgg taagctacag gatttcaggg
     8881 cgtcctgaat caccgccacc gttgcagcgt tagtgcgaca ggtttctttg ccaccgcgca
     8941 ggatcaccgc gttacctgtt ttcaggcaca gcgaagcgac atcaaccgtc acgttcgggc
     9001 gcgcttcata aatcacgcca ataaccccca gcggtacgcg acgacgctca agacgcaggc
     9061 cgctgtccag tacgccgcca tcgattacct gccccaccgg atcggcgagg ttgcacacct
     9121 ggcgcacatc atcggcaatg cctttcagcc gtgcgggcgt cagtgccaga cggtcaagca
     9181 tcgcttcgct gaggccattg gcacgcgcgt cagcaacatc ctgggcgtta gcgttgagga
     9241 tgatttcgct ttgtgcttcc agttcatcgg cgattttatc cagcacgcga tttttttcgc
     9301 ggctggagag ttgcgctaat ttatacgagg cttgcttcgc ggcaatgccc atttgttcca
     9361 gcatcagcct gctccttaac gggtaatcat gtcatcacgg tgaacggcaa ccgggccgta
     9421 ttcatatccc agtattgcat caatttcttg cgagtggtgt ccggcaatac ggcgtaatgc
     9481 atcgctgttg taacgactga cgccgtgggc gatatcgcga ccttcgaggt tacaaatgcg
     9541 gatgacttca ccacgcgaga agttgccagt cacgctttta atgcctttcg gcaacaggga
     9601 gctgccgcgt tcaagaatgg cggcggttgc cccttcatct accgtgattt cacccgcagg
     9661 cggcgcaccg aaaatccagc gtttacggtt ttcaagcgga gtcgcctggg catggaacag
     9721 cgtaccgacg gaaatgcctt ccatcacatc accaatgacg cccggcttgc tgcccgcggc
     9781 aataatggtg tcgatacccg cacggcaagc cacgtccgcg gcctgcaatt tggtactcat
     9841 gccgccagtt ccgaggcctg aaacgctgtc accggcaatc gcgcgcagtg cgtcatcaat
     9901 accgtaaaca tcttttatca attctgcctg cggattactg cgcgggtcag cggtatacaa
     9961 acctttttga tcggtcagta acaacagttt atcggcaccc gccagaatcg ccgccagtgc
    10021 agaaaggttg tcgttatcgc cgactttaat ctctgccgta gcgacagcat cgttctcatt
    10081 gattaccgga acgatattgt tatcgagcaa cgcacgcagg gtgtcgcggg cgttcaggaa
    10141 gcgttcacgg tcttccatat cagcacgagt cagcagcatt tgcccgacgt gaatgccata
    10201 aatcgaaaac agctgttccc acagttgaat cagtcgactc tgccctaccg ccgccagcag
    10261 ttgtttcgag gcgatagtcg ctggcagttc cgggtaaccc aggtgctcac gtccggcggc
    10321 gatcgcgccc gacgtcacaa taacaatccg atgcccggcg gcatgtaact gcgcgcactg
    10381 gcgaacaagt tcaacgatat gggcacggtt cagacggcgc gatccgcctg ttagcacact
    10441 ggtgccgagt tttaccacca gcgtctggct gtcactcatg attctctgcc attcaatttt
    10501 aggaaaaatg atatcaaacg aacgttttag caggactgtc gtcggttgcc aaccatctgc
    10561 gagcaaagca tggtgttttg ttgcgcggga tcagcaagcg tagcggcagt tgtttacgct
    10621 tttattacag atttactaaa ttaccacatt ttaagaatat cattaatctg taatatatct
    10681 ttaacaatct caggttaaaa actttcctgt tttcaacggg gctctcccgc agaatattcg
    10741 cgcgttaatt aaaatcagga atgaaaatga aaaagagcac tctggcatta gtggtgatgg
    10801 gcattgtggc atctgcatcc gtacaggccg cagaaatata taacaaagac ggtaataaac
    10861 tggatgtcta tggcaaagtt aaagccatgc attatatgag tgataacgac agtaaagatg
    10921 gcgaccagag ttatatccgt tttggtttta aaggcgaaac acaaattaac gatcaactga
    10981 ctggttatgg tcgttgggaa gcagagtttg ccggtaataa agcagagagt gatactgcac
    11041 agcaaaaaac gcgtctcgct tttgccggat tgaaatataa agatttgggt tctttcgatt
    11101 atggtcgtaa cctgggcgcg ttgtatgacg tggaagcctg gaccgatatg ttcccggaat
    11161 ttggtggcga ctcctcggcg cagaccgaca actttatgac caaacgcgcc agcggtctgg
    11221 cgacgtatcg gaacaccgac ttcttcggcg ttatcgatgg cctgaactta accctgcaat
    11281 atcaagggaa aaacgaaaac cgcgacgtta aaaagcaaaa cggcgatggc ttcggcacgt
    11341 cattgacata tgactttggc ggcagcgatt tcgccattag tggggcctat accaactcag
    11401 atcgcaccaa cgagcagaac ctgcaaagcc gtggcacagg caagcgtgca gaagcgtggg
    11461 caacaggtct gaaatacgat gccaataata tttatctggc aactttttat tctgaaacac
    11521 gcaaaatgac gccaataact ggcggctttg ccaataagac acagaacttt gaagcggtcg
    11581 ctcaatacca gtttgatttt ggtctgcgtc catcgctggg ttatgtctta tcgaaaggga
    11641 aagatattga aggtatcggt gatgaagatc tggtcaatta tatcgatgtc ggggctacat
    11701 attatttcaa caaaaatatg tcagcgtttg ttgattataa aatcaaccaa ctggatagcg
    11761 ataacaaatt gaatattaat aatgatgata ttgtcgcggt tggcatgacc tatcagtttt
    11821 aatgattatt gccggatgtg atgcatccgg cacatttcac tcacgccgtt aacttcaccg
    11881 gctcgtcacg aaaatcatcc gccggttcca gcttcagatt cagcgtcgtc agcagttcac
    11941 gcagtttctc gtgaaactca cgcagggtgt gctccagtcg ttcaaccact tcagtgtctt
    12001 ttaccggaac gctcttccag tcgcctgctt tatcgaacag accaaactgg taactgtagg
    12061 taaaacggga ttcctgcgct tcaagctcca tccaccagcc ccagaactca cgcacttccg
    12121 gtgccggttt cacgttgacg catacagcca gacaatcgaa aaagaatcga ttatcttcgc
    12181 acttaccttc acgaatatac gggcctagtg cggtaaattt tttgatcaat ctgctcttcg
    12241 ggtgtccact cggtaacgtc attgctatct cctgttgtga tgcaactgtt ttaccaaatt
    12301 ggcaaaattt agcaaatttt taacacaagc gtttttcgat ccagtcggtg atttcctgaa
    12361 gacctttgtc aaaattccga tacaccgggt taaatgggat ctctaataat ttaccgtcag
    12421 cagatgatga ggtgattaag cgtgagtcct cttccgggct gaacggatcg ttcttccagt
    12481 agcctgataa cattggcgtt gggcagcgac gtcccagcaa tccttgcact tttaatgaat
    12541 agcgattcag ctccacgcgc aacgcttcat cggaagcatc atgcatcccc aaacgactcg
    12601 ccagaacgtc aagatacatt tccggcacct gttgctggca cttaaaatca ctcaacaggg
    12661 tatgaactac cggaccaaga caggcaaccg ctttcagacg cggcgattca agatatgcca
    12721 gacgcacggc aacgttagcg ccgaaacgga aaccaaaggc cgcgacgcga gtgtgatcca
    12781 cccacggtac gttaggcagc gcctttaaga cgtgctgatg caacaggctg gagtcctggg
    12841 tgagcttcca ttttgatgaa aagcccaccg acggcatatc aatagtcagc atcgcaatgc
    12901 cgcgcggcgc aaaataacgt tcatacaggc tgtaatagtc cgtctgcatc gcatccagac
    12961 caccacacat taatactgtc gggaacgggc catcgccttt cggcatatgc aaaaagccgg
    13021 tgatgggcgc accgccgggt acggtaaact ccatctgccg catcgtgccc ggcagacgct
    13081 gagcggcctc ttcataggcg cggtttgaca aagcctgcgc ttgctcggcc aggtcatctc
    13141 ctttcagatg aggataggcg gcaatgttgt acaacgtagc cgcatgcagc cagtgacgac
    13201 cactgagttg cggatcttct tccgcacagg ctttttgttg ccacaccatc gcctgggtgg
    13261 cccactcata aatccagttg ccgccacggt agccaatcac cgtatcgtat aaatcatcgt
    13321 cggtacgttc ggcatcgctc atcacaatac gtgcctggac gtcgaggatt tcgcgtgggt
    13381 caatgccgcg ccagatccac atcagacggt taatcatgcg ataccagtga gggatggttt
    13441 taccatcaag ggccgactgc acaggcggtt gtgcgccgtg attaaagcgg cggactagcg
    13501 tcgaggtttc aggatgttta aagcggggtt tgaacagggt ttcgctcagg tttgcctgtg
    13561 tcatggatgc agcctccaga atacttactg gaaactattg taacccgcct gaagttaaaa
    13621 agaacaacgc ccggcagtgc caggcgttga aaagattagc gaccggagat tggcgggacg
    13681 aatacgacgc ccatatccca cggctgttcg atccaggtat cttgcgggat atcaacaaca
    13741 taatcatcaa ccagcggacg accagccggt tttgcaaaga tggtgacaaa gtgcgctttt
    13801 ggatacattt cacgaatcgc aaccgcagta ccaccggtat ccaccaggtc atcaataacg
    13861 atgaagcctt cgccatcgcc ttctgcgcgt ttcagcactt taagctcgcg ctggttgtcg
    13921 tgatcgtagc tggaaataca aacggtatcg acatgacgaa tacccagttc acgcgccagt
    13981 aacgcacccg gtaccagacc gccacggctt acggcaataa tgcctttcca ttgttcagaa
    14041 ggcatcagtc ggcttgcgag tttacgtgca tggatctgca acatgtccca ggtgacgatg
    14101 tatttttcgc tcatgtgaag tgtcccagcc tgtttatcta cggcttaaaa agtgttcgag
    14161 gggaaaatag gttgcgcgag attatagaga tctggcgcac taaaaaccag tatttcacat
    14221 gagtccgcgt ctttttacgc actgcctctc cctgacgcgg gataaagtgg tattctcaaa
    14281 catatctcgc aagcctgtct tgtgttgaca acattttctg ctaaccctgt gacctgcaat
    14341 actgttttgc gggtgatcga caaggagact taacgtgtct gaactgtctc aattatctcc
    14401 acagccgctg tgggatattt ttgccaaaat ctgttctatt cctcacccgt cctatcatga
    14461 agagcaactc gctgaataca ttgttggttg ggcaaaagag aaaggtttcc atgtcgaacg
    14521 cgatcaggta ggtaatatct tgattcgtaa acctgctaca gcaggtatgg aaaatcgtaa
    14581 accggtcgtc ttgcaggccc acctcgatat ggtgccgcag aaaaataacg acaccgtgca
    14641 tgacttcacg aaagatccta tccagcctta tattgatggc gaatgggtta aagcgcgcgg
    14701 caccacgctg ggtgcggata acggcattgg tatggcctct gcgctggcgg ttctggctga
    14761 cgaaaacgtg gttcacggcc cgctggaagt gctgctgacc atgaccgaag aagccggtat
    14821 ggacggtgcg ttcggcttac agagcaactg gttgcaggct gatattctga ttaacaccga
    14881 ctccgaagaa gaaggtgaaa tctacatggg ttgcgcgggg ggtatcgact tcacctccaa
    14941 cctgcattta gatcgtgaag aggttccagc tggttttgaa accttcaagt taaccttaaa
    15001 aggtctgaaa ggcggtcact ccggcggtga aatccacgtt ggcctcggta atgccaacaa
    15061 actgctggtg cgcttcctgg cgggtcatgc agaagaactg gatctgcgtc ttattgattt
    15121 caacggcggc acactgcgta acgccatccc gcgtgaagcc tttgcgacca ttgctgtcgc
    15181 ggctgataaa gtcgacgccc tgaaatctct ggtgaatacc tatcaggaga tcctgaaaaa
    15241 cgagctggca gagaaagaga aaaatctggc cttgttgctg gactctgttg cgaacgataa
    15301 agctgccctg attgcgaaat ctcgcgatac ctttattcgt ctgctgaacg ccaccccgaa
    15361 cggtgtgatc cgcaattccg acgtggcaaa aggtgtggtc gaaacctccc tgaacgtcgg
    15421 tgtggtgacc atgactgaca ataatgtaga aattcactgt ctgatccgtt ctctgatcga
    15481 cagcggtaaa gactacgtgg tgagcatgct ggattcgctg ggtaaactgg ctggcgcgaa
    15541 aaccgaagcg aaaggtgcat atcctggctg gcagccggac gcaaactctc cggtgatgca
    15601 tctggtacgt gaaacctatc agcgtctgtt caacaagacg ccgaacatcc agattatcca
    15661 cgcgggcctg gaatgtggtc tgttcaaaaa accgtatccg gaaatggaca tggtttctat
    15721 cgggccaaca atcaccggtc cacactctcc ggatgagcaa gttcacatcg aaagcgtagg
    15781 tcattactgg acactgctga ctgaactgct gaaagaaatt ccggcgaagt aattatttga
    15841 tttgctgccg gatggcgttt aatcgccttc cggcagtttc atccttcatt atccttcgat
    15901 aaaagccatc cctgtaaacg ttcgtcgcgg gttgccacgt tcaatctggt gatggaacat
    15961 tcgccgctgt gatttcagcg ccgcgctatt ttcctgttgc tgctgctcca gcctccaggc
    16021 aatcagcaat cgcgccagcc gtttgttagc atgctggctg cgctctgact gaaccttcac
    16081 gctaataccg gatgccagat gcgtggcgcg taccgccaag tcggttttat tgacattttg
    16141 accgcccggc cccgacgaac gcagcgtgtc aggataacgc ccggcttcgg tttccagcac
    16201 cgttaccgcg acgtcttgcc gggtaaaaac gaccaacaga gaagaacgct gcgccaatcg
    16261 ggtagccgcg tccggggtgt gtaaatctgg catccccact acgcgctgca tgttgggtaa
    16321 atttgccgtg gtgtgtaatt gttggatcgc taaactctcg atccacaggt catcagatgc
    16381 gatggtaaat accgcatcgg ataagggacg aatatatttg cccatgtacc attccattta
    16441 tgaataaaaa tcaggaaatg gcagacgaat ccagggcaag aaaaaaagaa ttaatcctgg
    16501 aaaggtctgc aaagagtcat tgataaagga tttgtcatga tttacctcct tgcagtttgt
    16561 ggaatcagaa aacgcgcaca taccaggcgg gcgttatttt cattgcaagc tggatttaat
    16621 gttgcggttt atatcgcata taaaaattag taaaccacgc tccccggcat tcaacgcgct
    16681 gctgcttaac tgtctgaaaa ccataacgtt caaaaaaggg ttttgcggtt atgctggcgt
    16741 ccaccgtaag ttcggattca gacttaatcc aaggttttaa caaagcactg gcaaccccac
    16801 ggcgggtgta ttcagggtca acaaataaca tatcgatata atgttcaatg cgggaaataa
    16861 aaccaaccgg ttgtgcatta atgactgcaa cccgcacttg tgatttcgcg agtttctcct
    16921 tccagcgaga ttcgtcaatc tgcgcccagg cagcaatttg ttgtggtgaa taatgctgac
    16981 tggctgtcat cgtaaccgct ctaatgaaaa tagcgcatag ttgctgaaaa tcgccaggct
    17041 gatagtttct tatttgtatg ttattcataa tataaattca aaaacgcatg cgaaacgctt
    17101 ctgccatttt cccaatttta tgcatttggt tgttatctcg agccagttta tgaggttcag
    17161 gtttcagaat ggcaatgagc aaccatgctt gctcatcaaa cgcaccctga caatatacca
    17221 aatgcgcttc gtcattcgtt ctgctgaatt ggcgcaactg tggcggaaat ggattattca
    17281 catttgccag atgaatatga gcaactcgct caaatttgat taatggccag gtaaacgagt
    17341 cgtcgtagag tgcatcgcga ccaaatatat ctggcaaaac accgtcacgc ttataggaaa
    17401 taaaatccgc cgttaacgca tcaagttcct ctgctgtaag ttgcaggcga ataagttttg
    17461 ttttgaatac ccgcatcctt attccttaaa gtcattgaaa tcatcatccg tcatatcatt
    17521 aagatattgt tcagcgcggc gtgtaatttc ctctaatctt gcagcacttg gacggaagcg
    17581 cgcctttatg ggctttttct cctcttgttc agcaagcatg tccattaacg cttcgaatgc
    17641 gctggcactt aagagatatc ctgcggggcg attattagaa agaaccgcaa ccggttgatc
    17701 aataaagtat ttagctgggt ttttacgtaa ctcagtgata ttgaccgatt tttcagcgag
    17761 aattcgatgc atacagtgat cccctcataa taatgcacac cagaatagac ataatagtat
    17821 acatcataat cccagcacca gttgtctttc catttgaggg tcaagcaacg tcacatgcag
    17881 ccccaccagt cgcacaccgc gcccaccgcg gcgttcatcc caggttttac gcgcggtggc
    17941 gattagatca gctttattca gccgcggcca gacgtgctcc tgggtggttt gctgaaaatc
    18001 gtcgaacttt aatttcaccc cctggcgagc aatcagtaaa tcaggtttta cctttgccag
    18061 acggcgttca agttccggat acagccgctc gataatcgct tcacattcag accagtgatg
    18121 aatatcttcc gccatcgtgc gttccacgcc gacggatttt cgcaaccgtt cgctgttaac
    18181 atcgcgttcg tcaatcccct gactacgctc ccacaaaatg cggccaaatt tgccaaagcg
    18241 tttaagcagc atcaccagat cacacttttg cacatcaccg caggtccgca gccccatcgc
    18301 ttccagtttt gccgccgaga ctttgccgac gccggggatt tttgccagcg gtaaggtttg
    18361 cagaaatgcc ggaacttctg ccggagtaat cacaaactgg ccgttgggtt tattcatgtc
    18421 ggaggcgatt ttggcgagaa actttaccgg cgcaacgccc gcagacgccg tcagttgcag
    18481 ctcgttgaaa atcgtctggc ggatttcctg ggcaatgagg gtcgcagaac cgtggcaatg
    18541 gacgctgtcg gtgacatcga gataagcctc atccagtgac aacggctcaa tgcgagaggt
    18601 gtagcgcgag aagatttcac ggatatgatt tgaggcttct ttgtaggcgt caaagcgccc
    18661 cggaagcaag gtgagatgtg ggcataattt gagcgccatc cctgtcggca tagcgctgcg
    18721 tacgccaaat ttacgcgcgg gataattggc ggtgctgatc accccccgac gttcgcggct
    18781 gccgccaata gcaataggga tatcgcgcag ggcgggattg tcgcgcatct ccactgcggc
    18841 gaaaaagcag tccatatcca catgaatgat tttacgcatt actcacctct caacactggt
    18901 aaagtataca gtgatttcag ggtttgagaa atgcgtaaag attcagcatt taacgcgttc
    18961 gctgcgaaag cacctgctgc ttatccagct tttgcaccag cggctgcacc actttatccc
    19021 catgctgacc aaagtattga tacagcgtat cggacgcact tttggtcagc accataatct
    19081 caatacgccg attgcccgcg ctttgcggat ttttggcatc cagcagcatc tggtccgcca
    19141 ttgcgcttac ctgcatcact ttatcttccg gcattccggc ctcttccagc acccgacgag
    19201 ctgaaagcgc gcgatcgccc gaaaggttcc agttgttgta gatattgttt ttgtaggcca
    19261 tcgcatcggt atgcccggta ataataattt tgttatcgag cgagtcgaac actggcgcga
    19321 gctccaccag caaagtttta aagaacggca taatctgcgc gctgccgcgt tcgaacatat
    19381 tgcgattctg gtcgtcttta atcagcacgc gtaatccctg cgggacaatc tccatctcca
    19441 gattcgcttc catatgcgca tcacgggcaa tggtgttgat actggttgcc aactcgccaa
    19501 gctcggtggc ggatttcttc ttcagcagcg ccgttttctc attcacatca cgcgcttttt
    19561 tctccgtctc ttccggcacc gccacggtgg caggttttgg cggatgagac gggctgattt
    19621 tatttaacgg cgacagccca ccaccgttaa aaatcgactg cccgtgcagc gcggcgataa
    19681 tgctttcacg ctcggattta ctgacgctgt tgacaatcaa ctttatcgtc gtcaccaaag
    19741 gggccgagcg tatttccgag gtttctgccc gctttaccct ggacgcgatg cccggcaaac
    19801 agatggcgat tgacgccgac cttaacgccg ggttgatcaa ccaggcgcag gcgcaaaccc
    19861 ggcgtaaaga tgtcgccagc gaggccgatt tctacggcgc gatggacggg gcatcaaagt
    19921 ttgtgcgcgg ggacgccatc gccgggatga tgattctggc gatcaacctg atcggcggtg
    19981 tctgtatcgg gatcttcaaa tacaacctga gcgccgatgc tgccttccag caatatgtgc
    20041 tgatgaccat cggcgacggg ctggtggcgc agatcccttc cctgctgctc tccaccgcgg
    20101 cggcgattat cgtcacccgc gtcagcgaca acggcgatat cgcccatgac gtgcgccacc
    20161 agctgctggc aagcccgtcg gtgctctaca ccgccaccgg gattatgttt gtgctggcgg
    20221 tggtgccggg aatgccgcat ctgccgtttt tgctgttcag cgccctgctt ggctttaccg
    20281 gctggcggat gagcaaacgc ccgcaggcag cggaggcgga ggagaaaagt ctcgaaacgc
    20341 tgacccgcac catcaccgaa accagcgagc aacaggtcag ttgggaaacg ataccgctga
    20401 tcgagcctat cagtttaagc ctcggttaca agctggtggc gctggtggac aaagcccagg
    20461 gcaacccgct cacccagcgt attcgcggcg tgcgacaggt gatatccgac ggcaacggcg
    20521 tgctgctgcc ggagatccgc attcgggaaa acttccgcct taagcccagc cagtacgcta
    20581 ttttcatcaa cggcattaag gctgatgaag cggatattcc ggcggataaa ctgatggccc
    20641 tgccctccag cgaaacctac ggcgagattg acggcgtgct ggggaacgac ccggcgtacg
    20701 ggatgccggt gacctggatc cagcctgcgc agaaagcgaa ggcgctgaat atggggtatc
    20761 aggtgatcga cagcgccagc gtgattgcca cccatgtgaa taagattgta cgcagctata
    20821 ttcctgattt gtttaactat gacgacatca cacagttgca taaccgtttg tcgtcgatgg
    20881 caccgcgcct ggcagaagac ttaagcgcgg cgctgaatta cagccagttg ctgaaagtgt
    20941 accgcacact actgaccgaa ggcgtttccc tgcgcgatat cgtcaccatc gccaccgtgc
    21001 tggtcgccag tagcgcggtg actaaagatc atattctgct ggcggccgat gtacgcctgg
    21061 cgctgcggcg cagcattacc catccgttcg ttcgcaagca ggagctgacg gtgtatacgc
    21121 tgaataatga gctggaaaat ctgctcacca atgtggtgaa tcaggcgcaa cagggcggca
    21181 aagtgatgct cgacagcgtg ccggttgacc cgaatatgct caaccagttc cagagtacga
    21241 tgccgcaggt aaaagagcag atgaaagcgg cggggaaaga cccggtgctg ctggtgccgc
    21301 cgcagttgcg tcctttgctg gcgcgctatg cgagattgtt tgcgccgggg ctgcatgtgc
    21361 tgtcgtataa cgaagtgccg gatgagctgg agttgaagat tatgggggcg ttgatgtagg
    21421 tatccggcat cggtgcacaa ccgccggatg cggcgtgaac gccttatccg gcctaccgat
    21481 ccggcacagg tttgtaggca tgataagacg cgccagcgtc gcatcaggca tccgcgcaca
    21541 attgccggat gcggcacaag tttgtaggca tgataagacg cgctagcgtc gcatcaggca
    21601 tccgcgcaca attatgaaat gatacgctcc ccggcactaa aatccgttat gttacccgcc
    21661 caatcaatgg gatataaccc tcgcgccaca tcgcgatgga acgttgagaa tggccaatca
    21721 ctcacttgct ttacccagcc atgttttact ggatttatat aaatataatc aacatgatgg
    21781 cgatagtctt tggtattgcg gatggtgtgc tcccaaaaac gtggttgcca gatatttttc
    21841 aatccacaag catgggtgaa ttgcttttta atctcccgcc agcgagagga aaaatcatca
    21901 tcgctttcag gtaatgtcca gatacagtgc atatgctccg gcaaaactac ccaggcatta
    21961 atttcaaaag gtctggctcg cttaacttta ataatggcgt tacggagcgt ctggtactgg
    22021 gtagtcaaaa gttggcttcg acgattccgt aaattcaccg tgaaaaacca gcttcccccc
    22081 ttgaggtaat aacgacgata ttcagacata gcgactcctt tttctcaacg gtaagaagaa
    22141 aaggaaaggt tggcaatgag gttgttttat atcgggaaag cggctcgcaa atattcgcgg
    22201 gctcgtaggc atgataagac gcgccagcgt cgcatcaggc atcggtgcac aactgccgga
    22261 tgcggcgtaa acgccttatc cgtcctacaa aatcgtctct tccgtcaaaa tccggcgcgc
    22321 gcctaaaaaa tgatcctgcc agaaaggttc agctaaccga cttacccgaa tggtttcccc
    22381 ggtgcgcggt gactcaataa actgaccgtc gcccagatac acccccatat ggtcggcaat
    22441 ctcgcggctg tggatatgga aaaacagcaa atctccccgg cgcaggtcgt tattcgccac
    22501 aatcgttgcc cggcggtagt gatacatctc attggccgtg cgcgggagct tcgcctcgag
    22561 gatcttgttg taggcataaa aaaccaaccc gctacagtcg aagcctttat cgggccgcgt
    22621 gccgccccag acgtaaggct tgccaagctg ctgctccagg cggtgaatcg ccacttcggt
    22681 gatgttatgc aggtgatcgc tgcttaaaaa gtggttgttt tccgccagcg cctgccagcg
    22741 tctgtcactc gccttgagcg gcgcaggaaa ccattctgga tgctgtttta tctgttcgcg
    22801 gttgtgctgg cgcagcgccc ttctgctttc ggcattgcct tccacaatat agctggcctg
    22861 ctttttcaga tgggtttggt actgttttaa taaacgcgca cggttttgca tccgctggcg
    22921 ggcggcgtag ctgaaagaga tatgtgatgt ggtggcgctg gcaaatgaag aaaacggcaa
    22981 acaaatcaac agtaatcccg acaatacaaa ggatggaatc gacggcaagg acatactcaa
    23041 taatggctgg tcatgcttta atgttgcagt gtatcccgga tcgtttctga acaaacttaa
    23101 taaaccatta ttataaaatt aacatatata tttcagtgaa ttatgtaaat acatcagttt
    23161 attgaattgc tcataaattg ccgctgttgc tcaattgagc tacaattcaa gctgaatata
    23221 tatacagcac aggagatacc ccaatggctg caaatgcatt tgttcgcgcc cgaatcgatg
    23281 aagatctgaa gaatcaggcg gcggacgtac tggccgggat ggggctgacc atctctgacc
    23341 tggttcgcat aaccctcaca aaggtcgcgc gtgaaaaggc attgccgttt gatttacgcg
    23401 agcctaatca attaaccatt caatcaatca aaaacagcga agctggcgtt ggtgttcata
    23461 aggccaaaga cgccgatg
//
LOCUS       DAZFKF010000076        23272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFKF010000076 DAZFKF010000000
VERSION     DAZFKF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.076"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            19..573
                     /gene="ybeT"
                     /locus_tag="ACHKA1_003519"
     CDS             19..573
                     /gene="ybeT"
                     /locus_tag="ACHKA1_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO5681117.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(583..2009)
                     /gene="djlB"
                     /locus_tag="ACHKA1_003520"
                     /pseudo
     CDS             complement(583..2009)
                     /gene="djlB"
                     /locus_tag="ACHKA1_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
     gene            complement(2006..2713)
                     /gene="ybeR"
                     /locus_tag="ACHKA1_003521"
     CDS             complement(2006..2713)
                     /gene="ybeR"
                     /locus_tag="ACHKA1_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO5681118.1"
                     /translation="MDMKSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQP
                     LADYPTCPETLKDMSDL"
     gene            2877..3854
                     /gene="ybeQ"
                     /locus_tag="ACHKA1_003522"
     CDS             2877..3854
                     /gene="ybeQ"
                     /locus_tag="ACHKA1_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO5681119.1"
                     /translation="MIFTSSCCDNLSIDKIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVAWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3924..4406)
                     /gene="ybeL"
                     /locus_tag="ACHKA1_003523"
     CDS             complement(3924..4406)
                     /gene="ybeL"
                     /locus_tag="ACHKA1_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO5681120.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4642..7224
                     /gene="leuS"
                     /locus_tag="ACHKA1_003524"
     CDS             4642..7224
                     /gene="leuS"
                     /locus_tag="ACHKA1_003524"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO5681121.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPQY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDSEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7239..7820
                     /gene="lptE"
                     /locus_tag="ACHKA1_003525"
     CDS             7239..7820
                     /gene="lptE"
                     /locus_tag="ACHKA1_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO5681122.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7820..8851
                     /gene="holA"
                     /locus_tag="ACHKA1_003526"
     CDS             7820..8851
                     /gene="holA"
                     /locus_tag="ACHKA1_003526"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO5681123.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8853..9494
                     /gene="nadD"
                     /locus_tag="ACHKA1_003527"
     CDS             8853..9494
                     /gene="nadD"
                     /locus_tag="ACHKA1_003527"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO5681124.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCDDLLPEPVLTYINQQGLYR"
     gene            9518..10129
                     /gene="cobC"
                     /locus_tag="ACHKA1_003528"
     CDS             9518..10129
                     /gene="cobC"
                     /locus_tag="ACHKA1_003528"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO5681125.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10389..10706
                     /gene="rsfS"
                     /locus_tag="ACHKA1_003529"
     CDS             10389..10706
                     /gene="rsfS"
                     /locus_tag="ACHKA1_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO5681126.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10710..11177
                     /gene="rlmH"
                     /locus_tag="ACHKA1_003530"
     CDS             10710..11177
                     /gene="rlmH"
                     /locus_tag="ACHKA1_003530"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO5681127.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11208..13109
                     /gene="mrdA"
                     /locus_tag="ACHKA1_003531"
     CDS             11208..13109
                     /gene="mrdA"
                     /locus_tag="ACHKA1_003531"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO5681128.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13112..14224
                     /gene="mrdB"
                     /locus_tag="ACHKA1_003532"
                     /gene_synonym="rodA"
     CDS             13112..14224
                     /gene="mrdB"
                     /locus_tag="ACHKA1_003532"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO5681129.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14235..15323
                     /gene="rlpA"
                     /locus_tag="ACHKA1_003533"
     CDS             14235..15323
                     /gene="rlpA"
                     /locus_tag="ACHKA1_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO5681130.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLSATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLRQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15463..16674
                     /gene="dacA"
                     /locus_tag="ACHKA1_003534"
     CDS             15463..16674
                     /gene="dacA"
                     /locus_tag="ACHKA1_003534"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO5681131.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16785..17048
                     /gene="ybeD"
                     /locus_tag="ACHKA1_003535"
     CDS             16785..17048
                     /gene="ybeD"
                     /locus_tag="ACHKA1_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO5681132.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17149..17790
                     /gene="lipB"
                     /locus_tag="ACHKA1_003536"
     CDS             17149..17790
                     /gene="lipB"
                     /locus_tag="ACHKA1_003536"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO5681133.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            18050..19003
                     /gene="ybeF"
                     /locus_tag="ACHKA1_003537"
     CDS             18050..19003
                     /gene="ybeF"
                     /locus_tag="ACHKA1_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO5681134.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRIQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19212..20177
                     /gene="lipA"
                     /locus_tag="ACHKA1_003538"
     CDS             19212..20177
                     /gene="lipA"
                     /locus_tag="ACHKA1_003538"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO5681135.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAAALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20278..20481)
                     /gene="tatE"
                     /locus_tag="ACHKA1_003539"
     CDS             complement(20278..20481)
                     /gene="tatE"
                     /locus_tag="ACHKA1_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO5681136.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20610..21398)
                     /locus_tag="ACHKA1_003540"
     CDS             complement(20610..21398)
                     /locus_tag="ACHKA1_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO5681137.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILALPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21491..21874
                     /gene="crcB"
                     /locus_tag="ACHKA1_003541"
     CDS             21491..21874
                     /gene="crcB"
                     /locus_tag="ACHKA1_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO5681138.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21928..22137)
                     /gene="cspE"
                     /locus_tag="ACHKA1_003542"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21928..22137)
                     /gene="cspE"
                     /locus_tag="ACHKA1_003542"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO5681139.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22312..22872)
                     /gene="pagP"
                     /locus_tag="ACHKA1_003543"
     CDS             complement(22312..22872)
                     /gene="pagP"
                     /locus_tag="ACHKA1_003543"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO5681140.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLANENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         6018 a         5829 c         5713 g         5712 t
ORIGIN      
        1 aaatcgaagc attcacacat gaataaaaaa ttaatgtata tattcgcaat ttttatagtt
       61 gcagcaatta cctgtattag ccaacccaag aaaacgacgt tgcgtgataa agccatggtg
      121 aattatgcct ttgattattt aagctcaccg ggcagtcttc cattcaccac ggcagccacg
      181 gagctttccg cgattcatgg tcactcaacg tcgcaatatc gccttggaga attttatctt
      241 catggtagcg acggtaaacc actggattat acacaggcga gatactggta tgagcaatca
      301 gcggaacagg aaaatccacg cgcgcaaagt aaactggggt ggatctacct caaaggtctg
      361 ggggtcaaac ccgacacccg taaagcaatt ctctggtata aggaagcagc tgaacaaggg
      421 tatgctcatg ctcaatatac tttaggtttg atctacagaa atggctcagg tattaatgtt
      481 aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta caaaaatgcg
      541 gaaagattac ttgccgggct tcccgcacat taacgtcgac tgtcacccga acagatggag
      601 tattttccct atcaaaccag caaagataaa aatatagaaa ataattttaa tgatgcccag
      661 acagcctggc ccaccatcgt caacgggcat tggctgcggt tgcggctccg gttcaacagt
      721 ttgcttctcg gtaaaattcg ccgcctgaat taataatcga cgaataatta atgcttccag
      781 tggacaacga ttttctgcat caagacggct tacgatcggc attaatgatt cttctgttgg
      841 ttggctggcc tgcactattg tttggcaact aaatataatg gccataaggg atgttctgta
      901 tcacgcgtga gtgagatccg cggcttacgt cctttgtgca aaaccatctc gaccagagaa
      961 cgccccagcg cagagagttt ttcatcactg agtatttcgt ataaatgaaa acgcgtacga
     1021 gcgctgtcac cagggacata aagtagcggt tttccctgat cgttaaccag atcacgcgct
     1081 tcaatcatca tcaccactaa tgggtaataa tcgacccgat gctgacggca ccattgcaac
     1141 attaaagcat ccgcctgggt ggagggatat tcgcgccagt agtcacaaag ttctgccagc
     1201 aggcttttgc cttctccaca atagacgcgt tgcgcgtatt cataataacg cgcatcttca
     1261 ttatcaggtt cagcttcccg ccacgaccgg gcgtaatcca ggagttcggg aattcccagg
     1321 cgacctgcgc tataccagcg gagtaaattt ctgtgcagtt ttgcatcatc ggggataatc
     1381 actgcgcgat gttgcgctaa atacaatgtc agccattctg gatgcgatga ccaaatccga
     1441 gcgaacattt ggtaaaaatc gatggtgtca ttttgcacgg caacgggcag atgcagaatg
     1501 gtctggaagt taaaaacatt ccccttcgca atggcatata aaaaggattc cagttcttct
     1561 tcgtctattt ccccggtatt ttcctcctgt aaccaccgca atctttctgc taataacacc
     1621 acgcactcga aggataaatg ggcagtgttc atggctattg tgcacagcga ccagcgtaat
     1681 tcatcaatct catccatcga gcaataattt aattgctgaa tgaatcgctg ccaggcggag
     1741 ggcagaaaac gttcactatc agaggcaagt aacgcactaa aggcgagcag aatttcatgt
     1801 tctgctacct cacattcttc tggttgccaa acagatttag caggcgactg cgcaatccgg
     1861 agcgcttcct catacgcctg acgaagttgt ttaaaaccct gcggatcggt ttctggatga
     1921 aaggacggta acagcgccag ataagcgcgg cggataatat cgacgtcagt cgtttcctct
     1981 atgttgagga tcttccagca atttttcata ggtcgctcat atccttaagc gtttccgggc
     2041 atgtggggta gtcagccagt ggttgccagg gtaaggtcgc gtaatatgca tcgtcactgg
     2101 caaataattg ataaaacata tcattgccgg aatcgtcgta ttctttacgc gctaagaatt
     2161 cacgtaataa aggcagatta tcatcttcgg gagactgcca atattgccga ccaaaggtat
     2221 aaccggcaaa gtattgccgc cagttactat aataacgtag agcccgtaga tgaatacgcg
     2281 actggatcca tagactttct tcttcactca accagttatt aagtacgccc attcgactta
     2341 aaaaacccat ccggacataa tcccatgctt taattcctcc ctcaccacag cattcggctg
     2401 tgctggcgac aaattgcgca taagcctgac cttgctcatt taattcagca agatagtcac
     2461 gccattcaca tggagaataa cgaaaccagc ggtgataaaa ccctgccaac cgagcggcat
     2521 gaccgttatc gatcattcgg tgaattgtgc gcagcaggtc atcacggtca tcaattcccc
     2581 aggaggaaga aaggtcaaaa aagcatgtac ctaaatacat tttgggggat gaatgcgaag
     2641 gtaaacagca ttcattgcgt atttccatcg gagtggaaag tgcaaacaat attttttgcg
     2701 acttcatgtc catataattt atccttaaac attcatttag tgaatgtaaa ctacattacg
     2761 aggctcattt atttatactt ttctgacaca acataatacg cataaaataa gacttacgca
     2821 aaaacggaac acataaaaat ataatatgtt ttatctgcaa ccataaggaa atgataatga
     2881 tttttacgtc aagttgctgc gataatttat caatagataa gattatcgaa cgtgctgaaa
     2941 aaggcgattg cgaggctcaa tatattgttg ggttttatta taatcgcgat agcgcaattg
     3001 actctccgga cgacgaaaag gccttttact ggctgaagct ggccgctgag caaggtcatt
     3061 gtgaagcaca atactcctta gggcaaaagt ataccgagga taaaagccgt cataaagata
     3121 atgagcaagc catcttctgg ctgaaaaaag ctgccctaca aggccatact ttcgcttcca
     3181 acgcccttgg ctggatactg gatcgtggag aagaccccaa ctataaagaa gcggttgcct
     3241 ggtatcagat agccgcggag agcggaatgt cttatgcgca aaataatctt gggtggatgt
     3301 acagaaatgg caacggagtc gcacaagact atacgctggc atttttctgg tacaaacaag
     3361 ctgcattaca aggccatagt gacgcgcaaa acaatctggc cgatctttat gaagacggaa
     3421 aaggcgttgc tcaaaacgag acactcgccg cattctggta tttgaaaagc gcacagcagg
     3481 gtaatcggca tgctcagttt caaattgcat gggattataa cgctggcgaa ggggtggacc
     3541 aggactataa gcaagcgatg tactggtatc tgaaggctgc cgctcagggg agcgtcggcg
     3601 cttacgtcaa catcggttat atgtataaac acggacaagg cgttgagaaa gattatcagg
     3661 ctgcctttga atggtttacg aaagccgctg aatgcaatga cgccactgcc tggtataacc
     3721 tggccattat gtatcactac ggagaaggaa gacctgtcga tctccgacag gctctcgacc
     3781 tgtatcgtaa agttcagtca tccggaacca gagatgtcag tcaggaaatt cgtgagactg
     3841 aagatttact gtagagataa aaacaagggc cggggaattt cattcggccc atattctctt
     3901 tattgctgtg cgaaatcaga ctattacggc tcaaacgggc gtctctggaa ctggtcatga
     3961 ccacatttcg ggcatagcgt cagcacttcc ggtgtgtaga tcgggagatg gaagtgacat
     4021 ttctcgcaga ccagatttcc cagcccgacc acttctccgc tgtgataaac cccatgatga
     4081 ttgaggtcct ggaaaacttc gcgccattca agctgcgttt tatcggtgat gtctgccagc
     4141 tcctgccaca agctttcttt aatcacccgc ataaagacgc tgtcagattc ttctttcagg
     4201 ctctcttcat agctcatggc gaactcttcc aggtcacgtc tgacagctcg cgtcagctca
     4261 tcgacctcgg ttcgcgttaa ctcccctgtt tttattacgc gctcgcgcgc ctgttccacc
     4321 agtgcgtcga tatcacgttc gccattgcgc aggcgttcgc tcagtgacgc aaccagttca
     4381 cggtaatatt gagcaacctt gttcatcgtt tcatctcccg ggtcgttaac tgactataat
     4441 aatagacgtt ctttgttcct tgctttgcta atacgcccac aaactctgta aattaatgta
     4501 tgaataatcc gggcatcatt tcaacgtcga gtgcgcgaaa ggctgttttg acgcggacgt
     4561 ttgggctatg ctatgcggat ctgaaaaacc acctctaacg ctacttttgt agccgtaatg
     4621 aaaacaggac cactggctgc catgcaagag caataccgcc cggaagagat agaatccaaa
     4681 gtacagcttc attgggatga gaagcgcaca tttgaagtaa ccgaagacga gagcaaagag
     4741 aagtattact gcctgtctat gcttccctat ccttctggtc gactacacat gggccacgta
     4801 cgtaactaca ccatcggtga cgtgatcgcc cgctaccagc gtatgctggg caaaaacgtc
     4861 ctgcagccga tcggctggga cgcgtttggt ctgcctgcgg aaggcgcggc ggtgaaaaac
     4921 aacaccgctc cggcaccgtg gacgtacgac aacatcgcgt atatgaaaaa ccagctcaaa
     4981 atgctgggct ttggttatga ctggagccgc gagctggcaa cctgtacgcc ggaatactac
     5041 cgttgggaac agaaattctt caccgagctg tataaaaaag gcctggtata taagaagact
     5101 tctgcggtca actggtgccc gaacgaccag accgtactgg cgaacgaaca agttatcgac
     5161 ggctgctgct ggcgctgcga taccaaagtt gaacgtaaag agatcccgca gtggtttatc
     5221 aaaatcactg cttacgctga cgagctgctc aacgatctgg ataaactgga tcactggcca
     5281 gacaccgtta aaaccatgca gcgtaactgg atcggtcgtt ccgaaggcgt ggagatcacc
     5341 ttcaacgtta acgactatga caacacgctg accgtttaca ctacccgccc ggacaccttt
     5401 atgggttgta cctacctggc ggtagctgcg ggtcatccgc tggcgcagaa agcggcggaa
     5461 aataatcctg aactggcggc ctttattgac gaatgccgta acaccaaagt tgccgaagct
     5521 gaaatggcga cgatggagaa aaaaggcgtc gatactggct ttaaagcggt tcacccatta
     5581 acgggcgaag aaattcccgt ttgggcagca aacttcgtat tgatggagta cggcacgggc
     5641 gcagttatgg cggttccggg gcacgaccag cgtgactacg agtttgcctc taaatacggc
     5701 ctgaacatca aaccggttat cctggcagct gacggctctg agccagatct ttctcagcaa
     5761 gccctgactg aaaaaggcgt gctgttcaac tctggcgagt tcaacggtct tgaccatgaa
     5821 gcggccttca acgccatcgc cgataaactg actgcgatgg gcgttggcga gcgtaaagtg
     5881 aactaccgcc tgcgcgactg gggggtttcc cgtcagcgtt actggggcgc gccgattccg
     5941 atggtgacgc tggaagacgg taccgtaatg ccgaccccgg acgaccagct gccggtgatc
     6001 ctgccggaag atgtggtaat ggacggcatt accagcccga ttaaagcaga tccggagtgg
     6061 gcgaaaacta ccgttaacgg tatgccagca ctgcgtgaaa ccgacacttt cgacaccttt
     6121 atggagtcct cctggtacta tgcacgctac acttgcccgc agtacaaaga aggtatgctg
     6181 gattccgaag cggctaacta ctggctgccg gtggatatct acattggtgg tatcgaacac
     6241 gccattatgc acttgctcta cttccgcttc ttccacaaac tgatgcgtga tgcaggcatg
     6301 gtgaactctg acgaaccagc gaaacagttg ctgtgtcagg gtatggtgct ggcagatgcc
     6361 ttctactatg ttggcgaaaa cggcgaacgt aactgggttt ccccggttga tgctatcgtt
     6421 gaacgtgacg agaaaggccg tatcgtgaaa gcgaaagatg cggcaggcca tgaactggtt
     6481 tataccggca tgagcaaaat gtccaagtcg aagaacaacg gtatcgaccc gcaggtgatg
     6541 gttgaacgtt acggcgcgga caccgttcgt ctgtttatga tgtttgcttc tccggctgat
     6601 atgactctcg aatggcagga atccggcgtg gaaggggcta accgcttcct gaaacgtgtc
     6661 tggaaactgg tgtacgagca tactgcgaaa ggtgatgttg cggcactgaa cgttgatgcg
     6721 ctgactgaag atcagaaagc gctgcgtcgc gatgtgcata aaacgatcgc taaagtgacc
     6781 gatgatatcg gccgtcgtca gaccttcaac accgcaattg cggcgattat ggagctgatg
     6841 aacaaactgg cgaaagcacc gaccgatagc gagcaggatc gcgccctgat gcaggaagcg
     6901 ctgctggccg ttgtccgtat gcttaacccg ttcaccccgc acatctgctt cacgctgtgg
     6961 caggaactga aaggcgaagg cgatatcgac aacgcgccgt ggccggttgc tgacgaaaaa
     7021 gcgatggtgg aagactccac gctggtcgtg gtgcaggtta acggtaaagt ccgtgccaaa
     7081 atcaccgttc cggtggacgc aacggaagaa caggttcgcg aacgtgctgg ccaggaacat
     7141 ctggtagcaa aatatcttga tggcgttact gtacgtaaag tgatttacgt accaggtaaa
     7201 ctcctcaatc tggtcgttgg ctaagcgcgg gaggaagcgt gcgatatctg gcaacattgt
     7261 tgttatctct ggcggtgtta atcaccgccg ggtgtggctg gcatctgcgt gataccacgc
     7321 aggttccttc cactatgaag gtcatgatcc tggactcagg tgatccgaac gggccattaa
     7381 gccgtgcggt gcgtaaccag ttacgtctga atggtgtcga gttgcttgat aaagaaacca
     7441 cgcgtaagga cgttccatcc ctgcgtttag gtaaagtgag catcgcgaaa gataccgcat
     7501 cggtattccg taacggtcaa acagcagagt atcagatgat catgacggtg aatgcgaccg
     7561 tgttgatccc cggccgtgac atctacccga ttagcgccaa ggtcttccgt tcgttcttcg
     7621 ataacccgca aatggcgtta gcgaaagata acgaacaaga catgatcgta aaagagatgt
     7681 acgaccgtgc tgccgaacag ctgattcgta agctgccaag catccgtgct gcggatattc
     7741 gttccgacga agaacagacg tcgactacaa cggatactcc ggcaacgcct gcacgcgtct
     7801 ccaccacgct gggtaactga tgattcggtt gtacccggaa caactccgcg cgcagctcaa
     7861 tgaagggctg cgcgcggcgt atcttttact tggtaacgat cctctgttat tgcaggaaag
     7921 ccaggacgct gttcgtcagg tagctgcggc acaaggattc gaagaacacc acactttttc
     7981 cattgatccc aacactgact ggaatgcgat cttttcgtta tgccaggcca tgagtctgtt
     8041 tgccagtcga caaacgctat tgctgttgtt accagaaaac ggaccgaacg cggcgatcaa
     8101 tgaacaactt ctcacactca ccggacttct gcatgacgac ctgctgttga tcgtccgcgg
     8161 taataaatta agcaaagcgc aagaaaatgc cgcctggttt actgctcttg cgaatcgcag
     8221 cgtgcaggtg acctgtcaga caccggagca ggctcagctt ccccgctggg ttgctgcgcg
     8281 cgcaaaacag ctcaacttag aactggatga cgcggcgaat caggtgctct gctactgtta
     8341 tgaaggtaac ctgctggcgc tggcccaggc actggagcgt ttatcgctgc tctggccaga
     8401 cggcaaactg acattaccgc gcgttgaaca ggcggtgaat gatgccgcgc atttcacccc
     8461 ttttcattgg gttgatgctt tgttgatggg aaaaagtaag cgcgcgttgc atattcttca
     8521 gcaactgcgt ctggaaggca gcgagccggt tattttgttg cgcacattac aacgtgaact
     8581 gttgttactg gttaacctga aacgccagtc tgcccatacg ccactgcgtg cgttgtttga
     8641 taagcatcgg gtatggcaga accgccgggg catgatgggc gaggcgttaa atcgcttaag
     8701 tcagccgcag ttacgtcagg ccgtgcaact cctgacacga acggaactca ccctcaaaca
     8761 agattacggt cagtcagtgt gggcagagct ggaagggtta tctcttctgt tgtgccataa
     8821 acccctggcg gacgtattta tcgacggttg atatgaaatc tttacaggct ctgtttggcg
     8881 gcacctttga tccggtgcac tatggtcatc taaaacccgt ggaaacgctg gcgaatttga
     8941 ttggtctgac gcgagtcaca atcatcccta ataatgttcc tccgcatcgt ccccagccgg
     9001 aagcgaacag cgtacagcgt aaacacatgc ttgaactggc gattgccgac aagccattat
     9061 ttactcttga tgaacgcgag ctaaagcgca atgccccctc ttacactgcg caaacactga
     9121 aagagtggcg gcaggaacaa gggccggacg tgccgctggc gtttattatt ggtcaggatt
     9181 cattgctgac ctttccgacc tggtacgaat acgaaacgat actcgacaat gcacatttga
     9241 tcgtctgtcg gcgtccaggt tacccacttg aaatggcgca accgcaatac cagcaatggc
     9301 tggaagatca tttgacacat aacccggaag atcttcacct tcagcctgcc ggtaaaattt
     9361 atctggctga aacgccgtgg tttaacatct cggcgaccat catccgcgaa cgtttgcaaa
     9421 acggtgaatc atgtgatgat ttattgccgg aaccggtact gacttacatt aaccaacaag
     9481 gcttgtatcg ctgatacctg tcgttacgga gggcatgatg cgactgtggt taattcgtca
     9541 tggcgaaaca caagcgaatg tcgatggtct gtacagcggt catgcgccca cacccctgac
     9601 cgcacgcggt attgagcaag cgcaaaatct gcatacactg ctagatgatg tttcctttga
     9661 cctggtttta tgcagtgaac tggaacgggc acagcatacc gcgcgactgg ttctcagtga
     9721 ccgccagctc cccgttcata tcatccctga actcaacgaa atgttttttg gcgactggga
     9781 gatgcgacat catcgcgacc tcatgcaaga agatgccgaa aactatagcg cgtggtgcaa
     9841 tgactggcag catgctatcc ccacgaacgg tgaaggattt caggcatttt cgcaacgtgt
     9901 ggaacgcttt atcgcaaggc ttagtgaata tcagcactat cagaatattt taatcgtcag
     9961 ccatcagggc gtactgagtc tgttaattgc tcgtttaatt ggcatgcctg ccgagtctat
    10021 gtggcatttt cgcgttgacc aggggtgttg gagcgccatt gatatcaacc aaaaattcgc
    10081 gacgctacgc gtcctcaata gccgtgccat cggggtcgag aatgcatgac ttttctgttt
    10141 ttttacgggt aagccgcaac gaccattgac agccccgggc aggctgatat tctccgcagc
    10201 cagacttttt ccgccagaca cgactttgta gaaattgttt tacaaaaatg gcgatgcaat
    10261 ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc gattcggcga caattgtccc
    10321 gaaatcgcct ctggttcagg tatactgaca gaccattttt atctatttga ttcacccagg
    10381 gggaaaactt gcagggtaaa gcactccagg attttgttat cgacaaaatt gatgacctca
    10441 aaggtcagga catcatcgcc ttagacgttc agggcaaatc cagcatcacc gactgcatga
    10501 tcatctgtac gggtacgtcc agccgtcatg ttatgtccat tgctgaccac gttgtgcagg
    10561 agtctcgcgc agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc gccgactgga
    10621 ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca ggaagagagc cgtcgcctgt
    10681 atgaactgga aaaactctgg agttaatgcg tgaagctgca acttgtcgcc gtaggaacga
    10741 aaatgccgga ctgggtacaa accggtttta ccgagtacct gcgtcgtttt ccgaaagata
    10801 tgcccttcga gctgattgaa attccggccg gaaaacgcgg caagaatgcg gacatcaagc
    10861 gcatactcga caaagagggt gagcagatgt tggcggccgc aggcaaaaac cgcattgtca
    10921 ccctcgatat tccaggcaag ccctgggata cgccgcagtt agccgctgag ctggaacgct
    10981 ggaagctgga tggtcgcgac gtcagtctac tgattggcgg gcctgaaggg ttgtcgcctg
    11041 cctgtaaagc ggcggcggag cagagctggt cgctgtcggc gcttaccctc ccccatccgc
    11101 tggttcgcgt gctggtcgca gagagtctgt accgggcgtg gagcatcacc accaaccatc
    11161 cttatcaccg tgagtgataa gggagctttg agtagaaaac gcagcggatg aaactacaga
    11221 actcttttcg cgactatacg gcagagtccg cgctgtttgt gcgccgggcg ctggtcgcct
    11281 ttttggggat tttgctgctg accggcgtgc ttatcgccaa cctgtataat ctgcaaattg
    11341 ttcgctttac cgactaccag acccgctcta atgaaaaccg cattaagctg gtgcctatcg
    11401 cgcccagccg cggcattatc tatgaccgta acggtatccc tctggccctc aaccgcacta
    11461 tctaccagat agaaatgatg ccggagaaag tcgataacgt gcagcaaacg ctggacgctt
    11521 tgcgcagcgt ggtagatctg accgatgacg atattgctgc attccgaaaa gagcgcgcac
    11581 gttcacaccg tttcacctct attccggtga aaactaacct gaccgaagta caagtagctc
    11641 gctttgccgt caatcagtac cgttttccgg gtgtcgaagt taaaggctat aaacgtcgtt
    11701 actatcctta cggttcggcg ttgacccacg tcatcggcta tgtgtcgaaa atcaacgata
    11761 aagacgtcga acgcctgaat aatgacggca aactggccaa ctatgcggca acgcatgata
    11821 tcggtaagct gggcattgag cgttactatg aagacgtgct gcacggtcag accggttatg
    11881 aagaggttga agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa gtaccaccgc
    11941 aagccggaca cgatatttac ctgacgctgg atctcaaact ccagcaatat attgaaacgc
    12001 tgctggcggg tagccgcgca gctgtggtag tcaccgatcc gcgtacaggt ggggtgctgg
    12061 cgctggtttc cacgcctagt tatgacccaa acctgtttgt tgacggtatc tccagcaaag
    12121 attattccgc cttgctgaat gacccgaata caccgctggt gaaccgcgcc acacaggggg
    12181 tttatcctcc cgcgtctaca gtgaaaccct atgtggcggt ttcggcattg agcgccgggg
    12241 tgatcacgcg caatacgacg ctgtttgacc caggctggtg gcaactacca gggtcggaaa
    12301 aacgttatcg tgactggaaa aaatggggcc acggacgtct gaatgtcaca agatcgctgg
    12361 aagaatctgc ggataccttc ttctatcagg tggcctacga tatggggatc gatcgcctct
    12421 ccgaatggat gggtaaattc ggttatggtc attacaccgg tatcgacctg gcggaagaac
    12481 gttccggcaa catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa ccgtggtatc
    12541 agggtgacac cattccggtt ggtatcggtc agggttactg gacagcgacc ccaatccaga
    12601 tgagtaaggc actgatgatc ctgattaatg acggtatcgt gaaggttccg catttgctga
    12661 tgagcaccgc cgaagacggc aaacaggtgc catgggtaca accgcatgaa ccgcccgtcg
    12721 gcgatattca ttccggttac tgggagctgg cgaaagacgg tatgtacggt gttgctaacc
    12781 gccctaacgg tacggcgcat aaatactttg ccagcgcacc gtacaaaatt gcggcgaaat
    12841 ccggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg cacaaaattg
    12901 ccgagcgttt acgtgaccac aaactgatga ccgcctttgc gccatacaac aatccgcaag
    12961 tggctgtcgc catgattctg gaaaacggcg gcgcgggtcc ggcggttggt acgctgatgc
    13021 gccagatcct cgaccacatt atgctgggtg ataacaacac cgatctgcct gcggaaaatc
    13081 cagcggttgc cgcagcggag gaccattaat catgacggat aatccgaata aaaaaacatt
    13141 ctgggataaa gtccatctcg atcccacaat gctgctgatt ttactggcat tgctggttta
    13201 cagcgccctg gttatctgga gcgccagcgg tcaggatatt ggcatgatgg agcgtaaaat
    13261 cggccaaatc gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc ctccacgcgt
    13321 ttatgaaggc tgggccccct atctctatat catctgtatt attttgctgg tggcggtaga
    13381 tgctttcggt gccatctcta aaggcgctca acgctggctg gacctcggta ttgttcgttt
    13441 tcagccgtcg gaaattgcca aaatagccgt accactgatg gttgcgcgct ttatcaaccg
    13501 cgacgtttgc ccgccatcgt tgaagaacac tggcatcgcg ctggtgctga tatttatgcc
    13561 cacgctgctg gtggctgcac agcctgacct gggaacatca atcctcgttg cgctttccgg
    13621 tctgtttgta ctgttcctct ctggccttag ctggcgtctg attggcgtcg cagtagtgct
    13681 ggtagcggcg ttcattccga ttctgtggtt cttcctgatg catgattacc agcgccagcg
    13741 cgtaatgatg ctcctggacc cggaatcaga cccactcggc gcgggctatc acattattca
    13801 gtctaaaatt gctattggct ccggcggatt acgcggcaaa ggctggctgc acggcactca
    13861 gtcacagctt gaatttctcc ccgaacgcca tactgacttt atcttcgcgg tactggcgga
    13921 agagctggga ttagtgggca ttctgattct gctcgctctc tacattctgc tgatcatgcg
    13981 cgggctgtgg atagccgcca gagcgcaaac cacctttggt cgcgtcatgg ctggtggctt
    14041 aatgctgata ttattcgttt atgtcttcgt aaatattggt atggtaagcg gtattctgcc
    14101 ggttgtaggg gttccgctcc cactggtcag ttatggagga tcggcgctaa ttgtgctgat
    14161 ggctgggttc gggattgtaa tgtcaatcca cacccacagg aaaatgttgt cgaaaagcgt
    14221 gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg catcgcggca ggaatgctcg
    14281 cggcatgtac aagcgatgat ggtcagcaac agacggtaag tgtaccgcag cctgcggtat
    14341 gtaacggccc catagttgaa attagcgggg cggacccgcg tttcgaacca ctgagcgcga
    14401 cggcaaatca ggattaccag cgcgacggta aaagctacaa aatcgtgcag gatccgtctc
    14461 gatttagcca ggcgggactg gcggcaatct atgatgccga accaggcagt aacctgacgg
    14521 cctctggcga agctttcgat ccgacacagc tgacggcggc ccatccaacg cttccgatcc
    14581 ccagctacgc cagaatcact aacctggcta acgggcgaat gatcgtggtg cgcattaatg
    14641 atcgcggtcc ttacggcaac gaccgcgtta tttcgctttc tcgcgcagca gctgaccgtc
    14701 ttaacacgtc aaacaacacc aaagttcgta tcgatccgat tattgtcgcc caggatggtt
    14761 cgctttctgg tcctggtatg gcttgtacca cagtcgccaa acagacttac gccctgcctg
    14821 cacctcccga tttaagcggt ggcgcgggaa caagttcagt gtctggcccg cagggtgaca
    14881 ttcttccggt cagtaattcg acgctaaaaa gcgaagatcc gaccggcgcg ccggtaacca
    14941 gcagcggttt cctcggcgca ccaacgacct tagcgcctgg tgtactggaa ggcagcgaac
    15001 cgacgcctgc gccacagccc gttgttacag ctccgtcgac aacgcctgca acctcgcctg
    15061 caatggtgac accgcaagcc gcctcgcaaa gcgccagcgg caactttatg gtgcaggtcg
    15121 gggccgttag cgatcaggct cgtgcgcaac agtaccaaca gcaactgaga cagaagttcg
    15181 gcgtccccgg tcgcgtaact caaaatggcg cagtctggcg gatccagctt ggcccattcg
    15241 ccagcaaagc agaagccagt accttgcagc aacgtttgca aaccgaagcc caattacagt
    15301 catttattac caccgcgcag tagcgtaaag caggcatctg atgtgtcaat ttctgtaaat
    15361 gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt gctatagtag
    15421 ggcacttttt ttaattccat cacggatgtc gtagttcaga ccatgaatac cattttttcc
    15481 gctcgtatca tgaagcgcct ggcgctcacc acggctcttt gcacagcctt tatctctgct
    15541 gcacatgccg atgacctgaa tatcaaaact atgatcccgg gtgtaccgca gatcgatgcg
    15601 gagtcctaca tcctgattga ctataactcc ggcaaagtgc tcgccgaaca gaacgcagat
    15661 gtccgccgcg atcctgccag cctgaccaaa atgatgacca gttacgttat cggccaggca
    15721 atgaaagccg gtaaatttaa agaaactgat ttagtcacta tcggcaacga cgcatgggcc
    15781 accggtaacc cggtgtttaa aggttcttcg ctgatgttcc tcaaaccggg catgcaggtt
    15841 ccggtttctc agctgatccg cggtattaac ctgcaatcgg gtaacgatgc ttgtgtcgcc
    15901 atggccgatt ttgccgctgg tagccaggat gcttttgttg gcttgatgaa cagctacgtt
    15961 aacgccctgg gcctgaaaaa cacccacttc cagacggtac atggtctgga tgctgatggt
    16021 cagtacagct ccgcgcgaga tatggcgctg atcggccagg cattgatccg tgacgtaccg
    16081 aatgaatact cgatctataa agaaaaagaa tttacgttta acggtattcg ccagctgaac
    16141 cgtaacggcc tgttatggga taacagcctg aatgtcgacg gcatcaaaac cggacacact
    16201 gacaaagcag gttacaacct tgttgcttct gcgactgaag gccagatgcg cttgatttct
    16261 gcggtgatgg gcggacgtac ttttaaaggc cgtgaagccg aaagtaaaaa actgctaacc
    16321 tggggcttcc gtttctttga aaccgttaac ccactgaaag taggtaaaga gttcgcctct
    16381 gaaccggttt ggtttggtga ttctgatcgc gcttcgttag gggttgataa agacgtgtac
    16441 ctgaccattc cgcgtggccg catgaaagat ctgaaagcca gctatgtgct gaacagcagt
    16501 gaattgcatg cgccgctgca aaagaatcag gtcgtcggaa ctatcaactt ccagcttgat
    16561 ggcaaaacga tcgagcaacg cccgctggtt gtgttgcaag aaatcccgga aggtaacttc
    16621 ttcggcaaaa tcattgatta cattaaatta atgttccatc actggtttgg ttaaaaattg
    16681 aacacttgaa agtgtaattt ccgtccccat atactaagca tcagtaaaaa aactcccgcc
    16741 ttctggcggg agttgctatt taattacgtt acgccggagc tgacatgaaa accaaactta
    16801 acgaactgct tgaattccct actcctttta cttacaaagt tatggggcag gcgttacctg
    16861 agctggttga tcaggtggtt gaagtggtac agcgccatgc gccaggtgac tacaccccaa
    16921 cggtaaaacc aagcagcaaa ggcaactacc actcggtatc tatcactatc aacgccactc
    16981 atatcgagca ggttgaaaca ctgtatgaag aactgggcaa aatcgatatt gtccgcatgg
    17041 ttctgtaact cgcttctccg ttacccggca cctggtcggg taactcccct ctcctcgctg
    17101 tgatatactt ttcccccact tttactcatt ctccacggag atgccgtttt gtatcaggat
    17161 aaaattcttg tccgccagct cggtcttcag ccttacgagc caatctccca ggctatgcat
    17221 gaattcaccg atacccgcga tgatagtacc cttgatgaaa tctggctggt cgagcactat
    17281 ccggtattca cccaagggca ggcaggaaag gcggagcaca ttttaatgcc tggtgatatt
    17341 ccggtgattc agagcgatcg cggtgggcag gtgacttatc acgggccggg gcaacaggtg
    17401 atgtatgtat tgcttaacct gaaacgccgt aaactcggtg tgcgtgaact ggtgaccttg
    17461 cttgagcaaa cagtggtgaa taccctggct gaactgggta tagaagcgca tcctcgggct
    17521 gacgcgcctg gtgtctatgt tggggaaaag aaaatttgct cactgggttt acgtattcga
    17581 cgcggttgtt cattccacgg tctggcatta aacgtcaata tggatctttc accattttta
    17641 cgtattaatc cttgtgggta tgccggaatg gaaatggcta aaatatcaca atggaaaccc
    17701 gaagcgacga ctaataatat tgcaccacgt ttactggaaa atattttagc gctactaaac
    17761 aatcctgact tcgaatatat taccgcttaa tttcatacct caatggccca atttacactg
    17821 ggtcattact cataacacct gctaatgctt tacatctcct tcgcgtttgt atattttctt
    17881 tatcacttgc agaaatgcga tgctacgcct tgctccgtaa ctcaccccat tgattaactt
    17941 attgaaatag ttgattatga tgagcaaaag cgttccgttc caattagaca taagattatt
    18001 gtaatttttt taaatattat ttttccatga ataaaattgg agtaagtgcg tggatagtaa
    18061 taatcaaatc gaaccctgtt taagcagaaa gtcgtcagaa ggtaaaccac aaatatttac
    18121 aaccttacga aatattgatc ttaaccttct gactattttt gaagctgtat atgtacataa
    18181 agggatcgtt aatgcagcga aagtgcttaa tctgaccccc tcggcaatca gtcagtctat
    18241 acagaaactg cgcgttatat tccctgaccc attgtttatt cgcaaaggcc agggtgtcac
    18301 tcctaccgca tttgcgatgc atctacatga gtatatcagt cagggccttg aatccattct
    18361 tggcgcgctg gatatcgaag gaagctatga taagcaacga acgataacta tcgcgaccac
    18421 cccctcggtc ggagccctgg tccttcctgt catttatcgg gcgattaaaa ctcactatcc
    18481 gcagctttta ctgcgcaacc cgcccatcag cgacgcggaa aaccaactca gtcagtttca
    18541 aaccgatctc atcatcgata acatgttttg caccaatcgt acggtgcaac atcatgttct
    18601 gttcaccgac aatatggtgt taatttgccg cgagggaaat ccactactct ctttagaaga
    18661 tgacagagag actatcgaca acgctgcaca tgtactcctg ttaccggaag gacaaaattt
    18721 cagcggtctg cggcagagaa ttcaagagat gtttccggac cggcagatca gcttcaccag
    18781 ctacaacatt ttgacaatcg ctgcactggt tgccaacagt gacatgttag cgattattcc
    18841 cagccgtttt tataacctgt ttagccgttg ctggccgctg gaaaaactgc cttttccgtc
    18901 cttaaatgag gagcaaatag atttctctat ccactacaac aaattcagcc tgcgtgatcc
    18961 catcctgcac ggagtaatag atgttatccg taatgcattt tgaaaaagta ttgttacagc
    19021 cagatcacag cgcagaacga taaaatggca caatccaaca acaattttac attttcgcga
    19081 ccgctttggc tgctttcagg tccgtttcaa tgatatactg ccagtcgtta attcaaaaat
    19141 agttgataat tacaacaatc tattgaattg aaacgctttc cttcgtaatt cgcaactgga
    19201 acacgcacgc tatgagtaaa cccattgtga tggaacgcgg tgttaaatac cgcgatgccg
    19261 ataagatggc ccttatcccg gttaaaaacg tggcaacaga gcgcgaagcc ctgctgcgca
    19321 agccggaatg gatgaaaatc aagcttccag cggactctac acgtatccag ggcatcaaag
    19381 ccgcaatgcg caaaaatggc ctgcattctg tctgcgagga agcctcctgc cctaacctgg
    19441 cggaatgctt caaccacggc acagcaacgt ttatgatcct gggcgctatt tgtacccgcc
    19501 gttgtccgtt ctgtgacgtt gcccacggtc gcccggtagc tcctgatgcc aatgagccag
    19561 tgaaactggc gcagaccatt gccgatatgg cgctgcgtta tgtggttatc acctccgttg
    19621 accgtgatga cctgcgcgat ggcggtgccc aacactttgc ggattgtatt actgccattc
    19681 gggaaaaaag cccgcaaatc aaaattgaaa ctctggtgcc ggatttccgc ggtcgtatgg
    19741 atcgtgctct ggatattctg actgcaacgc ccccagatgt gttcaaccat aacctggaaa
    19801 acgtaccgcg tatttaccgg caggtacggc ctggcgcaga ttacaactgg tcgctgaagc
    19861 tgctggaacg ctttaaagaa gcgcatccgg aaatcccgac caagtctggt ctgatggtgg
    19921 gtctgggtga aaccaatgaa gaaattattg aggtaatgcg cgacctgcgc cgtcatggtg
    19981 tgacgatgtt aacgctgggg caatatttgc agccaagccg ccatcacctg ccggttcaac
    20041 gttacgttag cccggatgag ttcgacgaaa tgaaagccgc agcgctggcg atgggcttta
    20101 cccatgctgc atgcggtccg tttgtccgct cttcttacca cgccgatttg caggcgaaag
    20161 ggatggaagt taagtaatcc tgtaataatt acttacatct gtctgataaa aaacccgctt
    20221 tttgaagcgg gttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
    20281 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
    20341 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
    20401 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
    20461 ggtaatacta atctcaccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
    20521 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
    20581 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
    20641 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
    20701 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
    20761 cggctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
    20821 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
    20881 caggcggcag gaagtgccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
    20941 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccttccac ctccagtaac
    21001 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
    21061 agatgcagtt tggcataacg ggcgacaatg tttcctgcct gtagcgccac cagcatattc
    21121 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
    21181 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
    21241 gatagatctg catcatgatc atcgcgtgcc agcaatgctt ccggcaggac aaacagcgat
    21301 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
    21361 acagatgtaa cagcaaactg tcctgcagca accagcattg ccacctcctg tcagagtcgt
    21421 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
    21481 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
    21541 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
    21601 gctggctgca aatctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
    21661 gatgacaaac attgatcccg tgtggaaagt attaatcacc accggatttt gtggcggtct
    21721 tacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
    21781 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
    21841 attctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
    21901 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
    21961 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
    22021 attagtctgg attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
    22081 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
    22141 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
    22201 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
    22261 ttaattattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
    22321 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
    22381 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
    22441 gtaattccag ttatcacgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
    22501 ttcattcgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
    22561 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
    22621 acgcgacagg ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
    22681 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
    22741 ctgttgccag gtttgtgcaa tattttctct aaacgttgtc atccactcat ctgcgttagc
    22801 aaaaacttta ccaacgctga ttaactgaat aaaaacaaag gaaaagatag cgacatattt
    22861 actcacgttc atttgtgacc ataaaacatt tatcaaaaat ctactactag catagcaaag
    22921 ctacaattaa cataacctta atagccccaa cataaagaat aatctgaata gctgctctat
    22981 ttatttccgc ttgttcaagt taaatatttc cgcgcatcga ttaaagatga ttcgtcgttt
    23041 tctgcgatgg gaatagtcat aaaagaaaaa gcaagttgtt tctgataaca aaatgcaacc
    23101 aagagaaaat tactttattt tttaacagca tacaaaaagc atcataacga agaatatatt
    23161 atatatcgtc aacccacgcc cattgcagat tattttttga gcacacaacc gcagctaaca
    23221 ccattgcaat taacaaattt gcatcaatcc accatcaatt tacacacatt at
//
LOCUS       DAZFKF010000077        22757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFKF010000077 DAZFKF010000000
VERSION     DAZFKF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.077"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            18..1040
                     /locus_tag="ACHKA1_003544"
     CDS             18..1040
                     /locus_tag="ACHKA1_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFO5681141.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSQ
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1040..2020
                     /locus_tag="ACHKA1_003545"
     CDS             1040..2020
                     /locus_tag="ACHKA1_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO5681142.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACTA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2017..2775
                     /locus_tag="ACHKA1_003546"
     CDS             2017..2775
                     /locus_tag="ACHKA1_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5681143.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEVLLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISH"
     gene            2785..3597
                     /locus_tag="ACHKA1_003547"
     CDS             2785..3597
                     /locus_tag="ACHKA1_003547"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5681144.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLISKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            3594..4448
                     /gene="modD"
                     /locus_tag="ACHKA1_003548"
     CDS             3594..4448
                     /gene="modD"
                     /locus_tag="ACHKA1_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFO5681145.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAIQNVLEW
                     SCGVSDYLDQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            4474..6444
                     /locus_tag="ACHKA1_003549"
     CDS             4474..6444
                     /locus_tag="ACHKA1_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO5681146.1"
                     /translation="MRLKKHYLCTALSLAFTQQAVAVQESDSLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINVTTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSELGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFVNEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(6494..6748)
                     /locus_tag="ACHKA1_003550"
     CDS             complement(6494..6748)
                     /locus_tag="ACHKA1_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO5681147.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            6949..7683
                     /locus_tag="ACHKA1_003551"
     CDS             6949..7683
                     /locus_tag="ACHKA1_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO5681148.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     VITPDKLVLDFGSQAEDNIAVLKARHITITAETQGAKVEFTVEQLQQSEYLQLPAFIS
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(7685..8296)
                     /gene="emtA"
                     /locus_tag="ACHKA1_003552"
     CDS             complement(7685..8296)
                     /gene="emtA"
                     /locus_tag="ACHKA1_003552"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO5681149.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8396..9310
                     /gene="ldcA"
                     /locus_tag="ACHKA1_003553"
     CDS             8396..9310
                     /gene="ldcA"
                     /locus_tag="ACHKA1_003553"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO5681150.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
                     GEGPICRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAMLNNTHEGTQLTISGHPVLKM"
     gene            9405..11141
                     /locus_tag="ACHKA1_003554"
     CDS             9405..11141
                     /locus_tag="ACHKA1_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO5681151.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            11262..11423
                     /locus_tag="ACHKA1_003555"
     CDS             11262..11423
                     /locus_tag="ACHKA1_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005023590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681152.1"
                     /translation="MDDKPLQALANKLKTPAQESQRNGFFRLQQVKNNQPRITGIPVN
                     HQGPQPDIA"
     gene            11762..13198
                     /locus_tag="ACHKA1_003556"
     CDS             11762..13198
                     /locus_tag="ACHKA1_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681153.1"
                     /translation="MASFKDICPFAPAIPPSSVNTGVRRPPGRLFLSVELKNQILQNM
                     KYLSPQKFSWGDAPWQIVDLSIAGKVNIQVDNNTIITLGTPLNQQHNEFMMVAKWCEW
                     AIQQDGLQENLQKKLHEVLEENRQNKQSEIPQEDLKERLKEIKEDILKENQSASQIED
                     RAEALRRMKECLITRQSMLDLSNLGLTSLPENLPPHLIEFNCSRNVLTALPEVMPKGL
                     RVLECMENFLILLPKVQPPKLMVLKCYENYIIWLPELSTNLRVINCSENFLQFLPPSM
                     PQYLYKLSCAGNNINSIPDEMLENLTRLKVFDCSSNDLISSPRLPPKLIIYYCGENQF
                     KTVRVPQPQSLKVFDCNGNPWDKDNLPTLLKAVEGLKNQQGLEDLLDFLRKEGQIDLE
                     GLEELEDLDDLMDLEFLDDPELLERVKVQEDLELLNQQLGLLSLERQQDSQPVNQQSE
                     HEPEPASRVKRDLSEVEFESTMKRKRFM"
     gene            complement(13367..14437)
                     /gene="dadX"
                     /locus_tag="ACHKA1_003557"
     CDS             complement(13367..14437)
                     /gene="dadX"
                     /locus_tag="ACHKA1_003557"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO5681154.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(14447..15745)
                     /gene="dadA"
                     /locus_tag="ACHKA1_003558"
     CDS             complement(14447..15745)
                     /gene="dadA"
                     /locus_tag="ACHKA1_003558"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO5681155.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            16075..17607
                     /locus_tag="ACHKA1_003559"
     CDS             16075..17607
                     /locus_tag="ACHKA1_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO5681156.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(17659..18378)
                     /gene="fadR"
                     /locus_tag="ACHKA1_003560"
     CDS             complement(17659..18378)
                     /gene="fadR"
                     /locus_tag="ACHKA1_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO5681157.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            18600..20141
                     /gene="nhaB"
                     /locus_tag="ACHKA1_003561"
     CDS             18600..20141
                     /gene="nhaB"
                     /locus_tag="ACHKA1_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO5681158.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            20287..20817
                     /gene="dsbB"
                     /locus_tag="ACHKA1_003562"
     CDS             20287..20817
                     /gene="dsbB"
                     /locus_tag="ACHKA1_003562"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO5681159.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(20863..22131)
                     /gene="umuC"
                     /locus_tag="ACHKA1_003563"
     CDS             complement(20863..22131)
                     /gene="umuC"
                     /locus_tag="ACHKA1_003563"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO5681160.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(22131..22550)
                     /locus_tag="ACHKA1_003564"
     CDS             complement(22131..22550)
                     /locus_tag="ACHKA1_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO5681161.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         5414 a         5971 c         5750 g         5622 t
ORIGIN      
        1 ttatcaggat ataacttatg tcactaacca gacggaggtt tacacagatt cttgcgtcga
       61 cgttgttcct gcatcatctg ccttcttttg cacaatcagt caaattctgg gcctcactga
      121 cgcttcccga agcccaaaac attacccgca tcgtcagcgc aggtgcgccc gccgatttgc
      181 tattgctggc tgtcgcgcca gaaaaaatgg tcggtttttc ctcttttgat tttgcccgtc
      241 aggcattaat tccattgcca gagcacattc gccagttacc caggctggga cgactcgccg
      301 ggcgcgccag cacactctcg caggaagggc ttatggcgtt acatcccgat ttggtcgttg
      361 attgcggcaa tacggatgaa acctggatct cccaggcacg gcaggttagc gaacagacac
      421 aaataccctg gttattgctt aacgggaaac tggagcaatc agcagaacag ttaacaacgc
      481 ttggcaaaac gttaggcgaa gagcatcgcg ccgcagaaca agccaatctc gccagccgct
      541 tcgttggtga agctcaggca ttcgccacct cacccgccgc taacctccgc ttttatgctg
      601 cgcgcggtcc tcgagggctg gaaacgggct tacagggttc gttgcatacc gaggcggcgg
      661 aattattagg tttgcacaac gtcgcgcaaa tagccgatcg ccacggcctg acacaggttt
      721 ccatggaaaa tctcctgcgc tggcagccgg atattattct ggttcaggag gccgttactg
      781 cagactttat tcgtcgtgat ccactctggc agggcgtgaa agccgttgcg gaacaacgca
      841 tcttattttt aagtggcttg ccctttggct ggctggatgc cccaccggga atcaaccgtc
      901 ttctgggatt acgcagactt cacgcctggc tggatcccgc catcaatcgc cagtttaaaa
      961 gtgacatgca gcattacgcc caactgttct ggcattgttc actcagtgac gccgactatc
     1021 aaaaattggt ggcgagctaa tgagaatcgt aaacggatgt attttgctgc tggcagctat
     1081 cagtatcact tttgccgccg taagtggggc ttatcatctc gatatgcagc aactgctggc
     1141 gctaattctt cgtcaagaaa atgttcctgt gcaggaacaa attgtcttct ggcaaatccg
     1201 cgtgccgcgt attcttgctg cgctgttact gggcgcggcg ctggcaggag ccggaaccac
     1261 ctatcaggga atgttgcgta atcccttagt ttcaccggat attttaggcg tctccgccgg
     1321 tgccggactt ggtgcctgca cggcaattct gtggggatta tccattgttt acatccagct
     1381 gtacgcattt tgcggcgggc tgatggtggt cgcgggcgtc tggttgatta cgcgtcgggt
     1441 gacccgccac gatccgatct taaccctggt gctggtaggc attgcgttag gaacgttgtg
     1501 cggagcgggg atatcgctaa ttaaaacgct ggccgatcct tacacccagt taccgtcaat
     1561 taccttctgg ttgctggggg gactgtccac cgtgacctta cgcgatctct gctatgctgc
     1621 accgatcatt ctcataggct ccttgcccct gttttttctc cgctggcgca tgaatttact
     1681 cacgctttcc gacgacgaag cccgttcgct gggactgaac gtgacgcgtc tgcgctttgg
     1741 attgattgtc tgcgccacac taatcaccgc cagtacggta gcaatagcgg gcataattgg
     1801 ttgggtcggc ctggtcgttc cgcacattgc ccgcttgttg accggtcata accatcagca
     1861 gctgttgccc atggcaatgt gtactggtgc gattctgctc ctgcttaccg atacgctggc
     1921 acgcagcatt ggaacaacag aaatcccact tggcattctg accgcttttg ttggcgcacc
     1981 gtttttcctg cttttgttgt tacgcggagg tcgccagtga ctttgcttgc cgtccgtcat
     2041 gcgtctctgg gttactcccg ccatccggtg ctacgcgacg tgtccttcac cctgtcgcaa
     2101 ggaacaattt gttgtctgtt aggagccaat ggttgcggca aaaccacact catgcgctcc
     2161 attcttggcg ttataccgtt attaaaaggc gaggtcttac tggatagcct ccctgtgcag
     2221 acactcagcc atcgccagcg cgcgcaggct attgcctggg tgccgcaggc gcacgacggt
     2281 atttttgctt ttagtgtgct ggatatggtg ttgatgggcc tggcccccac cataggcgct
     2341 ttttccgttc ccggtaagca agaacgcctg aaagccacgg agcaactgga aaagctgggc
     2401 attttgcatc tggcggcacg acgctggaat acgcttagcg gcggtgaacg tcagctggct
     2461 ttaatcgccc gtgcactcgt ccagcaaccg cgcttgttgt tactggatga gcctgcctcc
     2521 agcctcgatt tcggtcatca gatccaacta ctggacacgc tggcacagct caaaaataac
     2581 ggcatgacaa tgttaatgtc gactcatcat ccgctccatg ccaacgccat tgccgacagc
     2641 attatccagg tagagccaga tggacgagta acacaaggct taccgaccga acagttaaca
     2701 acaaacaaac tggctgccct gtatcgggtt tctgccgacc agattcatca tcacctctct
     2761 gcaattagcc actaaggaac aatcatgctc attgatgata ttgattttgc agacctctac
     2821 ctgcagcaac tcaaactggc ccaccgcaca gaaaaaacgc cggatcactg ggatcaacgg
     2881 gcggaaaaaa tggcggaaaa ttgcgcctcg cctacggaca gctacctgca acaactgatc
     2941 tcaaaaattg acctacaagg cgctcagacc ttattcgata tgggctgcgg accgggtacc
     3001 gtatcgctgg cgctggctga caaactcacg acgatttatg gcgttgatta cagtcagggg
     3061 atgctgaacg ttgccgcccg tcgcgccgcc gcgctgaagg cagacaatgt ccactggatc
     3121 cagcgcgcct gggaagagga ctggagtgac ctgccacgct gcgatattgc tgtggcttcc
     3181 cgctccactc tggtggctga tatgcgccag gcaatgagca aactgaataa tcaggcacgg
     3241 cttcgcgtct atacgactca tctggtcagc acctcgttcg tctctccagc cattcagcgg
     3301 gcggctggtc gggaagtgat cgaattaccc aactatatct ttgcgctcaa cgtcctgtat
     3361 cagatgggaa tttatgccca cgttgatttt atccgtggac aaaattgcca acaggataac
     3421 agcacctggg aacgttttga gcagaacgtc agctggagtc ttggcgcgct taacgacgat
     3481 gagcgtgagc ggttatatcg ctggtatcaa caacaagatg ctcgtgccct cgcccctgcc
     3541 agccgcgact gggcgctcat ctggtgggat agcgtaccgc aggaggcact gcgatgattt
     3601 tcctttctca ggcacaaatc gatgcgttac ttctggaaga tatccagggc ggtgacctga
     3661 ccacgagagc attaaatatt ggacaccagc atggctatat agagtttttt ctccgtcagg
     3721 gcggctgcgt cagcggcgtt tctgtcgcgt gtaagatgtt aactacgctg ggattaacta
     3781 ttgatgacgc ggtcagcgac ggttcacaag cgaacgcggg tcagcggcta atccgtgcgc
     3841 agggtaatgc cgcagcactt catcagggat ggaaggcaat ccagaatgtg ctggagtgga
     3901 gttgcggtgt ttctgattat ctcgatcaaa tgctggcgtt acttcgtgaa cgttatcctg
     3961 atggcaatat cgcctgcacc cgaaaagcaa ttccgggcac ccgcttactg gcctcgcagg
     4021 caattctggc tgccggagga ctgattcatc gtgccggatg tgcggaaacc atattattgt
     4081 ttgctaacca ccgccatttt cttcatgaca atcaggactg gtcaggcgca atcaatcagt
     4141 tacgtcgcca cgcacccgag aagaaaattg ttgttgaagc cgacacgccg aaagaggcaa
     4201 tcgccgcgtt acgcgcgcaa ccagacgtac ttcagctcga caaatttagt ccgcaacagg
     4261 caactgaaat tgcgcaaata gcgccgtcgc tggctcccca ctgcacgctg gctctgaccg
     4321 gcggaattaa tctgacgaca ctcaaaaatt acctcgactg cggcattcgc ctctttatta
     4381 cctccgcgcc ttattacgcg gcaccggctg acatcaaagt tagtctgcaa cccgcagcct
     4441 ctatttaatt ctttgttaaa cggaatttat tctatgagac tcaaaaaaca ttatctctgc
     4501 acagcattat cacttgcctt tacccagcag gccgtagcgg ttcaggagag cgactcgctg
     4561 accgtatggt ccagtccggt atcatcgacg acgaccaccg ttctcgatca acccaccatg
     4621 aaggccctgg ataaacagaa tgtcgctcag gcattaagtg tcgtccccgg cgtggtgctg
     4681 caaaagtcag gcagccgcaa cgaagaacag gtaaaagttc gtggctttga tagtcggcag
     4741 gtgccggtct atttcgacgg tgtgcccatt tatgttccct atgacggcaa cctcgatctg
     4801 gcgcggattc tgaccaacaa tctgggggca gtcgaagttt ccaaagggta ttcgtcgctg
     4861 cttcaggggc ctaatcagat gggcggagcc attaatgtca ccacccagaa gccaacgaaa
     4921 cctctggaag caagtctggg atatcgccag ggatggagcc gtagccagga caatgcctac
     4981 gatatgcatg cttcatttgc cgccagcagc gaactggggt atttgcaagt cagcggtagt
     5041 cagctaaagc aggattttct cggcctgccg catggtgtaa ataatgatat tgcaggcaaa
     5101 cacggcaaga tgattaattc atcggctgat gataaacgcg gcattgtgaa gctcggattt
     5161 acaccacgtg aaaacgatga atacacactg acttacatta agcaggatgg tgaaaaagat
     5221 aacccgccat acagcggaaa tagtggtcaa aaatcacgct actggcagtg gccagagtat
     5281 gacaaagaaa gtttttatta tcagggaacg acccaactaa acgatcgttt taccctgaaa
     5341 agtcggctgt atcgcgacac ctttgaaaat acgctgatga tgtacaactc gctggctgat
     5401 ttgaaaaata aaaaaggcag ctacagccat tattccgact acagcgacgg tgccggatta
     5461 caactggcag ccgatgtgcg tgaaaacgat ctgctgtcgt ttgccgttaa ctggaaagat
     5521 gacgtacacc gggaaaaagg tgcgccgcac gccgcttacg atcgctatga agatcgtacc
     5581 tggtcgctcg ccagtgaata tcaatgggct gctgccgata atgtcgatgt tgtggctgga
     5641 atcagctatg actggcgcga tagtgtagaa gcgaaaaaac acgagaaaga tggcagcatc
     5701 acccattatg acgacaacaa tcagtcagct tttaactggc aggtgatggg gaaataccac
     5761 tttgtcaatg aagacacgct ggcgctttcg tactatgacc gcacacgctt tccgacgctg
     5821 aaagaacgct ataccacgtc caaacctgcg tataaccaga tagcgattgt taacccgcag
     5881 ctcaagccgg aacgcgctcg cggggtggat ttaacctgga atggtgcctt cacgcgcgac
     5941 tggggatttg aggtcagcgt ttactataac cgggtgagtg atgccatcct ctcgcacaat
     6001 atcgatgccg ataccattca aaatcagaac agcggcacgg tggattacag cggtctggat
     6061 gccggtatta aggggaaaat cagcaatata ctggatgtag gattgagcta cgccctgatc
     6121 cacgctgacg ccaaacgcaa agacatcggc aagataaccg atctgccaac gcagacaatg
     6181 accgcatgga tgactctcaa accgtgggag ccgttaagcg taacgctgtc ggaagaggcg
     6241 cgttcctcca gctacagcaa cagtgacggt tcacaaaaag ccgccggttt tgcggtgacc
     6301 cacattcgag ccgattacac cttaggtcat ggcttcagcg tcaatgcgtc ggtcaataac
     6361 ctgtttgata cccaatatgc ctacagtgaa gggtttattg aagaaggccg caatttctgg
     6421 gcaggtgtcg agtatacgtt ctgatgtgaa agcggatagc ggcatctgta tcgctatccg
     6481 ccttatgaaa atcttattct cttcgcagga ggcggaatac gcccagaacg agaatagctc
     6541 ccaccaccgc caccaggaag ctgtgcaaat taaaaccgct gatggagccg ccaatgccaa
     6601 acatggtcgc cagccagccg ccgaccaccg caccgactat cccgagaata caggtcagga
     6661 aaaatccacc accatcacgc cccggcatga ttagcttggc gataatgccg gctatcaggc
     6721 caaaaataat ccaggcaata attcccatgt tcaggccctc gtttcgatga atcggacgca
     6781 agaaatgctt acgtcaacgc tctaagtata ggcaagggcg ggatcgatgc tgggcgtttg
     6841 tcggctgatt taagaacaac atgtaaaaaa tattcacgca tttgtattaa gttttgttaa
     6901 ctatgaccga taaaccaaag acagtttgtc agtcaggagt ttttccgcgt gagtcattac
     6961 catgagcagt tcctgaaaca aaatccgtta gccgtcctgg gcgtgttacg cgatttgcac
     7021 aaagccgcaa ttcctttgcg tatcagctgg aatggcgggc agctgatcag caaaatactg
     7081 gtaataaccc cggataaact ggtgctggat ttcggcagtc aagccgaaga caacatcgcc
     7141 gtactaaagg cacggcacat taccattacc gccgaaactc agggtgcgaa agtcgagttt
     7201 actgttgaac aactacaaca gagtgaatac ttgcagcttc cggcatttat tagcgtaccg
     7261 cctcccacct tatggtttgt acaacgacgc cgatatttcc gcatctccgc cccactccat
     7321 ccgccttatt tttgccagac caaactggcg gataacagta cgttacgttt ccgcctgtat
     7381 gatttgtcgt taggcggcat gggcgcatta ctggaaacag caaagcctgc cgggttgcac
     7441 gaaggtatgc gcttcgctca gattgaagtc aacatggggc aatggggtgt ttttcacttt
     7501 gacgcccagt taatctccat cagcgagcgc aaagtgattg atggcaagaa tgaaaccatc
     7561 accactcccc gtctgagctt ccgttttctt aacgtcagcc cgacggtgga gcggcaatta
     7621 cagcggatta ttttctctct cgagcgggaa gcccgggaga aagcggacaa agtacgcgac
     7681 tgatttacat cgcgtccagt gcctgctcaa gtttgtagat atagcgcgga gcctgcggcg
     7741 caggatgatt tcgcgctacg tgatcgagga actcgtcagc atccagatcg ttgattttgc
     7801 tgattgcctt tttccgatct gacgagaaag tccgtagcag cgcacctgcc ccgttagcgt
     7861 atgacaccac cagcgcatat tgcagtacct tcggatcttc aatgcctgcc agcgggccgg
     7921 tttccagaat attcaggtaa gccgccccca ttgaaatatt acgctcagga tttttcagct
     7981 cgctggtcgt cggctcacca ctccagccca tacggcgata tacatcacgt ccggaggttg
     8041 aagcttttaa ctgcatcaaa ccaatggcat tcgatttact caccgcgttc ggattaccac
     8101 ccgattcgat agcgataatc gccgtgatca attgtggatc gacgccccag gctgcaccgg
     8161 ctttctggct tattggcatc cactgcatcg cacgttgcac cggaactttc gcgttccacg
     8221 gcgggttcgt gtagtcatgc tttgatgaac aacccgctaa taacacaatc aaaaaggcaa
     8281 accatctcaa tttcacttct tctatcctta tagcctggaa tcgccgttga ggtgacaacg
     8341 gcacacctaa gcggcatgat atacatttcg tttctgttgt cagcaaggaa ttgccatgtc
     8401 tctgtttcac ttaattgccc catcgggtta ctgcattaaa cagcacgccg cgctgcgtgg
     8461 tatccagcgc cttaccgacg cggggcatca agtcaataac gtcgaggtta ttgcccgtcg
     8521 ttgtgagcgt tttgcgggta cggaaacgga gcgtctggaa gatctcaatt cccttgccag
     8581 actgaccacc cccaacacca tcgtgctggc tgtacgcggc ggttacggtg ccagtcgttt
     8641 actggcagat attgactggc aggcactggt ggctcgccaa caatatgacc cgttgctcat
     8701 atgcgggcat agcgatttta ccgccattca gtgcggtctt ctggcgcaag gcaatgtcat
     8761 cacctttagc ggccccatgc tggtagcaaa ctttggcgcg gatgaactga acacctttac
     8821 cgagcaccat ttctggctgg cgttacgcaa tgaaaccttt accattgaat ggcaagggga
     8881 aggcccgata tgtcgggccg aaggcacgtt gtggggaggc aatcttgcga tgctgatttc
     8941 actgatcggt acaccgtgga tgccaaaaat tgagaacggt attctggtgc tggaagatat
     9001 taacgagcac cctttccgcg tcgaacgtat gctgttgcag ctctatcatg ctggaatttt
     9061 gccacgtcag aaggcgatta ttctcggtag ctttagcggc agtacgccta atgattatga
     9121 cgcaggttac aacctggagt cagtgtacgc gtttctgcgt tcccgcctgt cgattccact
     9181 cattaccggc ctcgattttg gtcatgaaca acgcacggtt acgctgccgt taggcgcaca
     9241 tgccatgctt aataacaccc atgaaggcac tcagttgacg attagcggtc atcctgttct
     9301 taaaatgtaa aaaaatcacg cttcagggct aagtaaaacg ctgtctctgc cgctaatatg
     9361 gtagggttta tataagagac agcgtaatca ggagtaaccg accgttggat gccacaacaa
     9421 taattagcct tttcatctta ggatccatcc ttgtcaccag cagtatatta cttagttcat
     9481 tttcttcccg tcttggcatt cctattctgg ttatttttct ggcgatcggc atgctggcgg
     9541 gagtcgatgg cgtcggcggt atcccgtttg ataattaccc cttcgcctac atggtaagta
     9601 acctggcgct ggcgattatt ttgctcgacg gcgggatgcg cactcaggcc agttcctttc
     9661 gtgtggcgtt aggaccggcg ctgtcgctgg cgacgctggg cgtacttatc acctctggtt
     9721 taaccggcat gatggcggcg tggctgttta atcttgattt gattgaaggc ttattaatcg
     9781 gcgctatcgt cggctccacc gatgctgcag cggtcttttc tctgctgggt ggtaaggggc
     9841 ttaacgaacg tgttggctcg acgctggaaa ttgaatccgg cagtaatgat ccaatggcgg
     9901 tctttctgac gattaccctg attgcgatga tccagcagca tgaaagcagt gtcagctgga
     9961 tgttcgtggt tgatattctg cagcaatttg gcctcgggat tgtcatcggt cttggcggtg
    10021 gttatttact gctgcaaatg attaatcgca tcgccctgcc cgccggttta tatccattgc
    10081 tggcgttaag cggcggtatt ttaatttttg ctttaaccac ggcgctggaa ggtagcggta
    10141 ttctggctgt ttatctgtgc ggttttctgc tgggtaatcg gccgattcgc aaccgctacg
    10201 gcatcctgca aaatttcgac ggcctcgcct ggctggcgca aatcgccatg ttcctggtgc
    10261 tggggctgtt ggttaaccca agcgatctgc tgcctattgc cattccggcg ctcattttgt
    10321 ccgcatggat gattttcttc gcccgtcctc tttcggtatt tgccggattg ctaccattcc
    10381 gtggcttcaa tctgcgtgag cgcgtgttta tcagctgggt gggattacgc ggcgcggtgc
    10441 cgattattct ggccgtgttc ccgatgatgg cggggctgga gaatgcacgt ctgttcttta
    10501 atgtcgcctt ctttgtggtt ctggtttcac tgctcttgca gggaacatca ctctcgtggg
    10561 cggcgaaaaa agccaaagtg gtggttccgc ccgtcggacg tccggtgtca cgcgttggcc
    10621 tggatattca tccggaaaat ccgtgggagc agtttgttta tcaattgagt gccgataaat
    10681 ggtgcgtggg cgcggcactg cgcgatttgc atatgccaaa agagacgcgt attgcagccc
    10741 tgtttcgtga taaccagttg cttcatccta ccggcagcac ccgactgcgc gaaggcgatg
    10801 tattgtgtgt aattggtcgg gaacgcgatc tcccggcgct cggtaaactg ttcagccagt
    10861 cgccaccggt cgcgctggat caacgcttct ttggtgactt cattctcgaa gccagcgcca
    10921 aatatgctga tgttgcgctg atatatggcc tggaagacgg gcgagaatat cgtgataaac
    10981 agcaaacgct gggtgaaatt gtccagcagt tgttaggcgc ggcaccggtt gtcggtgacc
    11041 aggtagagtt tgccggaatg atctggacgg tggccgagaa agaagataat gaagtgttga
    11101 agattggcgt tcgagtggct gaagaggaag ctgaatctta aatttaatga agcatctatc
    11161 gccggttacg atgctttgct taacgcagcc tgagcctgta tataaacttg tcatttcctg
    11221 attttgatat gcacattcca acatcagaag aaggttaaat tatggatgac aaaccattgc
    11281 aggctctggc caataaactc aaaacaccgg ctcaagaaag tcagcgtaat ggcttcttcc
    11341 gactgcaaca ggtgaagaat aaccagcccc gcattaccgg tatccccgtc aaccatcagg
    11401 ggccccagcc tgacatcgcc tgaacatatt cccccacgca acctgccgca ctgacatagc
    11461 ccacacaata ggcgtttcat ccgcctgtag caccggatgc atgagcgatt tcttatagtt
    11521 attcctttgc ttaataacga ttatttccgg gtcgttcgct ctcgtctttt ctttaatacg
    11581 tcagagctac ctgggccgta atacagcatg taccagaccc gacacggcat ttctgaccag
    11641 ggaaaaagtc aacgatgagt agttatatca gcgcaaatga cgtcctgtgg ctcctttgcg
    11701 gtgaccacat tcacgtctcc ggcgctgcca gaggatattt atttcaagga tacgtagcat
    11761 tatggctagc tttaaagaca tctgcccatt cgcccccgcc attcccccat cttccgttaa
    11821 cacaggcgta cgacgcccgc cagggcggct cttcctgtcg gtggaactca agaatcaaat
    11881 tttgcagaac atgaagtatc tcagcccgca aaaattcagc tggggtgatg ccccctggca
    11941 gattgtcgat ctcagtatcg cgggcaaagt gaacatacag gtggataaca ataccatcat
    12001 cacgttgggg actcccctga atcagcaaca taacgagttc atgatggtcg ccaaatggtg
    12061 cgaatgggct atacagcagg atggtttgca agaaaacctg caaaaaaaac tgcacgaggt
    12121 cctggaagaa aaccggcaaa acaaacagtc agaaattccc caagaagacc tgaaagaaag
    12181 gctgaaagaa atcaaggaag atatcctgaa agaaaaccag tcagcaagcc agattgaaga
    12241 ccgggcagaa gcattgcgca gaatgaagga atgtctcata acaagacaga gtatgctcga
    12301 tcttagcaac cttggactga cttcactccc tgaaaatttg cctccacatc tgattgaatt
    12361 taactgcagt agaaacgtgt tgaccgcgtt accggaggta atgccaaagg ggctgagagt
    12421 gcttgaatgt atggagaact ttttgatctt gttaccgaag gtgcagcccc cgaaactgat
    12481 ggtactgaag tgctatgaaa actatattat ctggctgcct gagctgtcga ctaacctgag
    12541 agtgattaac tgttctgaaa acttcttgca atttttaccg ccgtcgatgc cccagtacct
    12601 gtataaactg tcctgtgctg gcaacaatat taactcaata cctgatgaga tgctggagaa
    12661 cttgactcgc ctgaaggtat tcgactgttc tagtaacgat ttgatctctt caccacggct
    12721 gccgcccaaa ctaatcatat actactgtgg agaaaatcag tttaaaactg tacgggtgcc
    12781 gcagccccag agcctgaagg tgtttgactg taatggtaac ccttgggaca aagacaattt
    12841 accgacgctg ctcaaagccg tcgagggcct gaaaaaccag cagggcctgg aggacctttt
    12901 agactttttg cgcaaggaag gccagattga cctggaggga ctggaggagc tggaggacct
    12961 ggatgacctt atggatctgg agttcctgga tgacccggaa ctcctggagc gcgtgaaggt
    13021 acaggaggac ctggaactcc tgaatcagca gttgggcctg ttgagtctgg aaagacaaca
    13081 ggactcgcag cctgttaatc aacaatctga acatgaaccc gaacctgcat caagggtgaa
    13141 gcgtgattta tctgaagtcg agttcgagtc aacaatgaaa cgtaagcgtt ttatgtagaa
    13201 cgcctgtgcg gtgagttcag gttccgggga aatagtgtcc atcattttaa tgggcactat
    13261 catgggcaat ggctctgtca cattaacaac tacagttgct gaccagccgg atggatgatt
    13321 ttttttgcaa ccagaagacg ttgcctccga tccggcttac aacaagttac accgtcacaa
    13381 ccgggacacg taacgccagc gcgcacatca actcatagcc caccgttccg gcagcggcgg
    13441 cgacatcatc aattttgatc tccttgcccc acagctcaac cggcgtacca atacccgcct
    13501 gcgggcaagg cgttaaatcg accgccagca tatccatcga aactgtccct actgtcatgg
    13561 tgcgcacgcc gtcaactaaa acaggggtgc cggtaggcgc atggcgcgga taaccgtcgg
    13621 cgtatcctgc ggcgacaatg ccgattcgct gttcatctcg cgcagtatag cgaccgccgt
    13681 agcccacacg ctcgcccgct tttagcgtct ggacaccaat aatctcactg cttagcgtca
    13741 tcaccggacg taatccggta ttggcgatat cacgccactg accggacggc gaagcgccat
    13801 acaaaataat gccaggccga acccagtcaa aatgcgcttc cgggtgccac agagtcgccg
    13861 ccgaattgga caacgaacgc cgacactcca gcccttccgc cgcctgctca atacgcgcca
    13921 tcgcgccgga aattccatca ggatgttccg cctcggcaaa atgcgacatc agggtcattt
    13981 cgccaacatt cgccattgcc cgcaactgct gccagacggt aagcacgcga tcgggctgga
    14041 agcccaaccg attcatccca ctgttcactt taagataaat atccaacggt gcttttagcc
    14101 gcgcattttg cagtgctttg agctgccagt tgctgtgtac gcaggtggtc aggcggtgct
    14161 ggtcataaat ctccagatcc tgagcatgga aaaatccttc cagcatcagg atcggtcctt
    14221 tccagccgcg ctcacgtaac gttattgcct cttccaggtt aagtaatgca aagccatcgg
    14281 tagccccgag cgcgctccag atacgctcaa taccgtgccc gtaagcgttc gcttttacca
    14341 ccgaccagac gcgcgcgtgc ggcgcggcct ggcggacaat ggacagattc tgttttaatg
    14401 cctgcagatc gaggctggcc tgtatcggac gggtcatctc gtttccttag ctgtgtgcgc
    14461 catgtaaatg gcccggacgt gatggcgtaa atccacggct gtagcgcgct acgcttagat
    14521 cctcatatgg gatcgctggt gtgcgaccag agagcaggtc gcttaacaac tgaccggaac
    14581 cgcaagccat cgtccagccg agcgtgccgt gaccggtatt cagccacaga tttttaaagc
    14641 gtgtacgccc gacaaccggc gtgccgtctg gcgtcatcgg gcgcagaccc gtccagaaag
    14701 tcgcctgctc gacatgaccg ccgcgtggat agagatcgcg aaccaccatt tctagcgttt
    14761 cacgacgcgg ttgcaacagc tcggtgttaa aaccaacaat ttccgccatc ccgccaacac
    14821 gaatgcggtt atcgaaacgg gtaatggcga ttttgtaggt ttcatcaaga atggtggata
    14881 ctggcgcacc atcttcctgc gcaattggaa tagtcagcga gtagcctttc agcggataca
    14941 ccggaatatc aacaatgcct ttgagcatcg ccgtcgagta agaaccaaac gccatcacat
    15001 acgcatcggc cttaatcact tcatcgccac acttcacgcc gtagatttgc tcgccgtcgc
    15061 aaagcagttg gtcaacgggc gtattaaagc ggaatttaac ccccgcctgc tccgccatcc
    15121 gcgccagatt ctgggtaaat agctgacagt ctccggtttc atcattgggt aactgcaggc
    15181 cgcccgtcag tttgtgcgcc acttctgcca gcgcgggctc cacttccgcc aggcggctgg
    15241 attccagcag ctgatacggt acgccggcat cttccagcac ggcgatatcg cgggtcgcat
    15301 tttcatactg ttgttcggta cggaacagtt gcagcgtccc accctgacgc ccttcatact
    15361 gaatattggt ttcggcgcgc aatgctttca ggcaatcacg gctgtattcc gccagacgca
    15421 ccatccgccc tttgttttcc atgtagtggc tggtgtcgca gttacgtaac atttgccaca
    15481 tccatttcaa ctggaactgc gtaccgtcga gacgaactgc cagcggcgca tggcgctgga
    15541 acatccattt gatcgctttt aaaggcacac ctggtgccgc ccacggtgca gcatatccgg
    15601 gggagatttg cccggcattc gcggcactgg tctccagggc ggcccccggc tcccgatcaa
    15661 tgacggtgac ctcatgtcct gcctgattta agtaccaggc gctggcaacg cctaccacac
    15721 cacttcccag tatgacaact cgcatagcca ctccgataac ggtagataca gtaaaagaat
    15781 aataatctaa ttacatcttg ataactcagt tgaaaatatt attcaacata cggctttttt
    15841 atggtgagct ggctcacatc tccctgactc tgcggcaata tggcgtcttt agtatctcct
    15901 gctgaacgcc ctttttatcc agcataaaat tccgttcaga agcggattag tggcactctg
    15961 ataataagaa atcgcaaaga aaaaaattct ccggcgacgc ggtttttaac aggtgttcta
    16021 tgcttgaaat gaggtgctcc acacagagag cgccgacaac aatgaggtgc gcgtatggcg
    16081 acgatcgatt ctatgaataa ggacaccaca cgtttgagcg atggacccga ctggacgttc
    16141 gacctgctgg atgtttatct ggcagagata gaccgggtgg cgaaactcta ccggctggat
    16201 acctacccgc accagattga agtgataacc tcagaacaga tgatggatgc ctactccagc
    16261 gtcggcatgc caattaacta tccgcactgg tcattcggta aaaagtttat cgagactgaa
    16321 cggctgtata agcacggtca gcaaggactg gcctatgaaa tcgtcattaa ctctaacccg
    16381 tgtatcgctt acctgatgga agagaacacc attaccatgc aagcgctggt gatggctcat
    16441 gcctgctatg ggcataactc tttcttcaaa aacaattact tattccgtag ctggaccgac
    16501 gccagttcga ttgtcgatta tctgattttt gcccgtaaat atattaccga gtgcgaagag
    16561 cgctatggcg ttgatgaagt agaacggctt ctggactcgt gccacgcgct gatgaactac
    16621 ggcgtggacc ggtacaaacg cccgcaaaaa atctcgctgc aagaagagaa agcccggcag
    16681 aaaagtcgcg aagagtatct gcaaagtcag gtcaatatgc tctggcgtac cctgccgaag
    16741 cgcgaggaag agaaaacggt tgctgaagcg cgccgctatc cgtccgagcc acaagaaaac
    16801 ctgctctatt ttatggagaa aaatgcgccg ctgctggaat catggcagcg tgaaatcctg
    16861 cgtattgtgc gtaaggtgag ccagtatttt tatccgcaaa aacagactca ggtgatgaac
    16921 gaaggctggg ccaccttctg gcactacacc atccttaacc atctgtatga tgaagggaaa
    16981 gtaacggaac gttttatgct ggagtttttg cacagccaca ccaatgtggt cttccagccg
    17041 ccctataaca gcccgtggta cagcggcatc aacccgtatg ccctcgggtt cgccatgttc
    17101 caggatatta aacggatttg tcagtcgcca acggaagaag acaaatactg gttcccggat
    17161 atcgccggtt ccgactggct ggaaacgctg catttcgcga tgcgtgattt caaagatgag
    17221 agttttatca gccagttcct gtcaccgaaa gtgatgcgtg atttccgctt cttcaccgtg
    17281 ctggatgatg atcggcataa ttatctggag atttccgcta ttcataatga agagggttat
    17341 cgggagatcc gtaaccggtt atcgtcgcaa tataacttaa gtaatctgga gccgaatatt
    17401 cagatctgga acgtggattt gcgcggcgac cgttcgctga cactgcgtta cattccacat
    17461 aatcgcgcac cgctggatcg ggggcgcaaa gaagtactga agcatgtgca tcgcctgtgg
    17521 ggatttgatg tgatgctgga acagcaaaac gaagacggca gcgtcgagtt gctggaacgt
    17581 tgcccgccga gaatgggaaa tctgtaaaaa caacaaaaaa cccctcgttt gaggggtttg
    17641 ttttttaaac ggaagggatt atcgcccctg aatggctaaa tcacccggca gatttttctg
    17701 catccggtgc caaatctcgc cactctcatg cccatagcga cgcactgttt cgtacacctg
    17761 atcgtgcgcg ccttcactgc acaacgccga cagtttgtgg tagaagccca gcgccagact
    17821 gcgcgcttcc ggattggcga aatagtgacg accaatgcgc gtatacagtc ccttcatccc
    17881 gttaaggatc agaccgtaaa tcgggttgcc ggaagcaaac gccaggccgc ggaatatgtt
    17941 gtaatccagc tcggcaaagg catcggcgtg atcggccact tcattagcgg tagccagcac
    18001 ttcctgcgct ttatcgggat gctgacgaaa cgcggtgcga ataaaaatag ttgaaatatt
    18061 ggtacgcacc gacagcaaat tatcaataag ctgcggcaca ctttcgtgat ccagtcgcgc
    18121 cagtgtttca aggatattta aaccagaggt ttcccagaaa ttattcacct tcgtcggctt
    18181 gccatgttga atggtcaacc agccatctcg tgccagacgc tgtaacactt cacgtaacgt
    18241 agtacgcgtt acgccaatta attctgaaag ttcacgttct gcgggcaaaa tagtcccggg
    18301 agggaagcgg ttattccaga tactttcaat aatgtactct tccgcgaaac ccgccgggct
    18361 ttgcgcctta atgaccatag tgagatttcc ataacacagc aaaacaaagt tggactcatc
    18421 ataccagagg gctaactacg ctgatagcag acaaaaagaa gaaaaaaggg atcaaggctt
    18481 cattttccgc atctctttcc ccttgactat caagacttgc ctcactcctg cctgaccgtt
    18541 aagctttgcg tttaaacata aaaacatcat tgattatttg taaggtaagg gaaaccatca
    18601 tggagatctc ctggggccgc gcgctatggc gcaatttttt gggccagtcc cccgactggt
    18661 acaaactcgc cctcatcatt ttcttaatcg taaatccgtt aattttcctc atcagccctt
    18721 tcgtcgctgg ctggttgctg gtagcggaat ttattttcac tctggcgatg gccctgaaat
    18781 gctacccgct actccccggt ggtctgttgg ctatcgaagc ggtattcatc ggcatgacca
    18841 gcgcggaaca cgtccgtgaa gaggtggcgg caaatcttga agtcttgctg ttattgatgt
    18901 ttatggtggc gggtatctat tttatgaaac agctgttgct gttcatattt acccgtttgc
    18961 tgttaagcat tcgctccaaa atgctgctgt cgctctcttt ttgcgtggcg gcagcattcc
    19021 tctccgcgtt cctcgatgcc ttaaccgtcg tggcggtggt gatcagcgtt gcagtcggtt
    19081 tttatggtat ttatcatcgc gtagcctctt cccgtaccga agacaccgac ctgcaagacg
    19141 atagtcatat cgacaagcat tacaaagtag ttttggaaca gttccgtggc tttctgcgta
    19201 gcctgatgat gcatgccggt gtcggcaccg cattaggcgg cgtgatgacc atggtgggcg
    19261 aaccacagaa cctgatcatc gctaaagcgg ctggctggca ttttggcgat ttcttcctgc
    19321 gcatgtcgcc ggtgaccgtt ccggttctga tttgtggcct gttaacctgc ctgctggtag
    19381 agaagctgcg ttggtttggc tacggtgaaa cgctgccgga gaaagtccgc gaagtgctgc
    19441 aacagtttga cgatcaaagc cgccaccagc gtacccgtca ggataaaatc cgtctgattg
    19501 tccaggcgat tattggcgtc tggctggtga ctgcgctggc gttgcatctg gcggaagttg
    19561 gcttgattgg tttgtcagtc attattctgg caacatcatt gaccggtgtc accgatgaac
    19621 atgctatcgg taaagccttc accgaatctc tgccattcac cgcactgttg acggtgtttt
    19681 tctcggtagt cgcggtgatt atcgaccaac aactgttttc gccgattatt cagtttgtgt
    19741 tgcaggcatc ggaacatgct cagctgtcgt tgttctatat tttcaacgga ctgctgtcat
    19801 ccatttcgga taacgtcttc gtggggacga tttatatcaa cgaagcgaaa gcggcaatgg
    19861 aaagtggcgc tatcacgttg aagcaatacg agctgctggc ggtcgccatt aataccggta
    19921 ccaatctgcc ctctgtcgct acgccgaacg gtcaggctgc gttcctgttc ctgctgacct
    19981 ctgcactcgc gccattgatt cgcctctctt atggccgcat ggtgtggatg gccctgcctt
    20041 acaccctcgt cctgacactc gtcggactgc tctgcgtcga gtttacgctt gcccctgtaa
    20101 ccgaatggtt tatgcaaatg ggctggatag caacgctttg ataacaactt accgggcata
    20161 taaatgcccg gtttgccttt tcgcccgata attgtccaat tgcgtcctat ttttgctgcc
    20221 tcctggtggc gcagcgaatg aattggttta aactgcgcac tctatgcata ttgcagggaa
    20281 atgattatgt tgcgattttt gaaccaatgt tcacaaggcc ggggcgcgtg gctgttgatg
    20341 gcgtttactg ctctggcact ggaactgacg gcgctgtggt tccagcatgt gatgttactg
    20401 aaaccttgcg tgctctgtat ttatgaacgc tgcgcattat tcggcgttct gggtgctgcg
    20461 ctgattggcg cgatcgcccc aaaaacgcca ctgcgttatg tagcgatggt tatctggttg
    20521 tatagtgcgt tccgcggtgt gcagttaact tacgagcaca ccatgcttca gctctatcct
    20581 tcgccgtttg ccacctgtga ttttatggtt cgtttcccgg aatggctgcc gctggataaa
    20641 tgggtgccgc aagtgtttgt cgcctctggc gattgcgccg agcgtcagtg ggatttttta
    20701 ggtctggaaa tgccgcagtg gctgctcggt atttttatcg cttacctgat tgtcgcagtg
    20761 ctggtggtga tttcccagcc gtttaaagcg aaaaaacgtg atctgttcgg tcgctaatcc
    20821 attcggcgct cctgcgggag cgcttttttc ctgccgctat atttatttga cccgcagtaa
    20881 atcagaactt cgcgttgtat aacgtggtga aagcatggct cgcttcatct gccattgttg
    20941 ctggatcccc tgcccggcaa aatagagtgt tcctctgccc tctttagcat tcagtgtatc
    21001 cattaccgtc atcaattgct cactcccggg gcgcggtgcg ttgtcatcga ataaattgag
    21061 ctgcgcgact ccctgactga agaaatcccc cagcatcacg cccgcttttt ggtagcgatg
    21121 gcccgcttgc cagatggcat ccagagatcg cgtagcggcg ttaatgatat ccctgctgtc
    21181 ctgagtgggc gtcagcagtt ttaccgacgc gctattgccg taataaggtt cattgagcgc
    21241 aaatggtgac gtcttaataa atgtggagat aaaccgacaa tattgatgct cactgcgaag
    21301 tttttccgcc gcccgggcag cgtaactaca aatggcctgc cgcatcgacg tataatccgt
    21361 gatgcgttca ccaaacgagc gggaacagat aatttcctgc tttgtcggtg caaattcttc
    21421 cagttgcaaa cagggttcac cgcgcagttc acgcaccgtt ctttcgagca cgacattaaa
    21481 atgtttacgg ataaaccgga tatctgtatc cgccaaatcg agaacggttt tgatccccat
    21541 cgcgtccagt tttttgctga tccgccgtcc aatcccccag acgtcatcca cggggagagc
    21601 agacattaat ttacgctggc gttccagatt tgataaatcc accaccccac ccgtctgccg
    21661 ctgccatttt tttgccgcat gattggcaag cttagccagc gttttggtct gggcgatccc
    21721 cacaccaaca gtaagatggg tacgttgtag caccgttgcg cgaatttctc tgccaaagtc
    21781 agtcagatcg cgacaattac gcacacctgt cagatcgcag aatgcctcat caatactgta
    21841 aatctcgacg cggggcgata gttcttccag cgtcgacatc acccgattgc tcatgtctgc
    21901 gtaaagctca taattgctgc taaagcaaac cacgccacag cgacgaaaca gatctttttg
    21961 tttgaaccag ggatcgccca ttttaacgcc aagcgccttt gcctcagcgt ttcgggcgat
    22021 aacgcaaccg tcattattcg atagcacaac caccggttta ccccataaat cagggcgaaa
    22081 caccgtctca cagctggcat aaaacgcgtt tacatcacag agggcaaaca tcagcgcatc
    22141 gccttaacga cgtggatcac cacaccaaag acatccagcg tatcttcact actgatggta
    22201 atgggcgagt acgcgctgtt catgggaata agctgtaccg tcgggcgtag ttgcaatttt
    22261 ttcaccgtaa actcgccgtc aacagcagcg atgacaatat caccatggct ggcggtaata
    22321 gcgctatcga caatcagtaa atcaccgtca ctaattccac catcaatcat agaatcacca
    22381 cttgctttga cgaagtaagt cgcgctggga tgctggatca acagttgatt cagatcgatg
    22441 cgctgttcaa cgtaatctgc ggccggtgaa ggaaagccac actgaacaag atcgctaaat
    22501 agcggaaaag tcacaatttc gcggagatcc gcaggcttga taaacaacat aataatctgc
    22561 ctgaagttgt actgttttta tatacagtag tctgttcttg ccagcagatc aatactgatt
    22621 caggctatca atatttgtcg ctgcatagac tgctgatttt tcgttctctt atcctgtgct
    22681 cacgcggcct tctggcgacg acgctcatcc agcagaaatg aaaaatatca cccggctaaa
    22741 aaatagaata gaagcat
//
LOCUS       DAZFKF010000078        22420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFKF010000078 DAZFKF010000000
VERSION     DAZFKF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.078"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..150)
                     /locus_tag="ACHKA1_003565"
     CDS             complement(<1..150)
                     /locus_tag="ACHKA1_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681162.1"
                     /translation="MPRTCILYAHPSTTAGCGVNALSGLRMAREFVGLIRRVSVASGI
                     CARLPD"
     gene            complement(157..1170)
                     /gene="mhpE"
                     /locus_tag="ACHKA1_003566"
     CDS             complement(157..1170)
                     /gene="mhpE"
                     /locus_tag="ACHKA1_003566"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO5681163.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAVDKLGWQHGADLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(1167..2117)
                     /gene="mhpF"
                     /locus_tag="ACHKA1_003567"
     CDS             complement(1167..2117)
                     /gene="mhpF"
                     /locus_tag="ACHKA1_003567"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO5681164.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLDANVEQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(2114..2923)
                     /gene="mhpD"
                     /locus_tag="ACHKA1_003568"
     CDS             complement(2114..2923)
                     /gene="mhpD"
                     /locus_tag="ACHKA1_003568"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO5681165.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(2933..3799)
                     /gene="mhpC"
                     /locus_tag="ACHKA1_003569"
     CDS             complement(2933..3799)
                     /gene="mhpC"
                     /locus_tag="ACHKA1_003569"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO5681166.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            complement(3817..4761)
                     /gene="mhpB"
                     /locus_tag="ACHKA1_003570"
     CDS             complement(3817..4761)
                     /gene="mhpB"
                     /locus_tag="ACHKA1_003570"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO5681167.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(4763..6427)
                     /gene="mhpA"
                     /locus_tag="ACHKA1_003571"
     CDS             complement(4763..6427)
                     /gene="mhpA"
                     /locus_tag="ACHKA1_003571"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO5681168.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            6618..7451
                     /gene="mhpR"
                     /locus_tag="ACHKA1_003572"
     CDS             6618..7451
                     /gene="mhpR"
                     /locus_tag="ACHKA1_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO5681169.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            7566..8342
                     /locus_tag="ACHKA1_003573"
     CDS             7566..8342
                     /locus_tag="ACHKA1_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFO5681170.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKLIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEVTMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIVA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            8362..9315
                     /locus_tag="ACHKA1_003574"
     CDS             8362..9315
                     /locus_tag="ACHKA1_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HFO5681171.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKHFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            9352..10434
                     /gene="lacI"
                     /locus_tag="ACHKA1_003575"
     CDS             9352..10434
                     /gene="lacI"
                     /locus_tag="ACHKA1_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO5681172.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEYLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            10557..13631
                     /gene="lacZ"
                     /locus_tag="ACHKA1_003576"
     CDS             10557..13631
                     /gene="lacZ"
                     /locus_tag="ACHKA1_003576"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO5681173.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFSAPEAVPESWLECDLPVADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLRGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQVIE
                     LPELPQPESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGRGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLPDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            13683..14936
                     /gene="lacY"
                     /locus_tag="ACHKA1_003577"
     CDS             13683..14936
                     /gene="lacY"
                     /locus_tag="ACHKA1_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO5681174.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            15002..15613
                     /gene="lacA"
                     /locus_tag="ACHKA1_003578"
     CDS             15002..15613
                     /gene="lacA"
                     /locus_tag="ACHKA1_003578"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO5681175.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSDV
                     EKRESLIKEMFATVGENAWVEPPVYFSCGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(15716..16897)
                     /gene="cynX"
                     /locus_tag="ACHKA1_003579"
     CDS             complement(15716..16897)
                     /gene="cynX"
                     /locus_tag="ACHKA1_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO5681176.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTTQVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLLLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(16903..17373)
                     /gene="cynS"
                     /locus_tag="ACHKA1_003580"
     CDS             complement(16903..17373)
                     /gene="cynS"
                     /locus_tag="ACHKA1_003580"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO5681177.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(17404..18063)
                     /gene="cynT"
                     /locus_tag="ACHKA1_003581"
     CDS             complement(17404..18063)
                     /gene="cynT"
                     /locus_tag="ACHKA1_003581"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO5681178.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            18173..19072
                     /gene="cynR"
                     /locus_tag="ACHKA1_003582"
     CDS             18173..19072
                     /gene="cynR"
                     /locus_tag="ACHKA1_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO5681179.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDMLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAVHEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHTALEECADVGENESR"
     gene            complement(19412..20695)
                     /gene="codA"
                     /locus_tag="ACHKA1_003583"
     CDS             complement(19412..20695)
                     /gene="codA"
                     /locus_tag="ACHKA1_003583"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO5681180.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKICAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRSWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(20685..21944)
                     /gene="codB"
                     /locus_tag="ACHKA1_003584"
     CDS             complement(20685..21944)
                     /gene="codB"
                     /locus_tag="ACHKA1_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO5681181.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGLADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT         5281 a         6204 c         5908 g         5027 t
ORIGIN      
        1 atccggcagt cgtgcgcaga tgcctgatgc gacgctaacg cgtcttatca ggcctacaaa
       61 ttctcgcgcc atccgtaggc cggataaggc gttcacgccg catccggcag tcgtgctggg
      121 atgggcatac aaaatgcacg ttctcggcag tcaatattat ttgttgttgc gcagatccag
      181 cgccacgtcg acgatcatat cctcctggcc gccaaccatc cggcgtttgc ccagctcaac
      241 gagaatatcc accgcgctta agccataacg cgccgccgcc gtttcacagt gacgcaggaa
      301 gctggagtaa acaccagcgt atcccagcgc cagcgtttcg cgatcgactc gtaccggtcg
      361 atcctgcaac ggacgcacca ggtcgtcagc ggcatccatt aacgcataga gatcggcccc
      421 atgctgccaa cccagtttat ccacggcggc aataaatact tccagcggcg cgttacctgc
      481 gcccgctccc attcccgcga ggctggcgtc gattcggtcg cagccctctt ccaccgccgc
      541 gatagagttc gccacgccaa gactcaggtt atggtgagcg tgcatgccgg tttgcgtttc
      601 tggtttcagc actgctttca gggcgcggaa acggtcacgg atatcgctca tgttcatcgc
      661 accgccagaa tccaccacat aaatgcaggt cgcaccgtag ccttccatca gctttgcctg
      721 cttggcgaga ttctccggcg tggtcatatg gctcatcatc agaaaaccaa cggtgtccat
      781 tccgagctcg cgggcatact gaatatgctg ggcggaaaca tcagcttcgg tacagtgcgt
      841 agccacacga accacccgcg cgccagcctg ccaggcattt ttcagatcgt gaatagtgcc
      901 gattcctggc agcaacaacg tcgcgatttt ggcgtgcttc accacatccg ccgctgcttc
      961 aatccattca aggtcgctat gtgcgccgaa accatagtta aagctggacc cctgcaaacc
     1021 gtcgccgtgg gccacttcaa tcgaatccac gcgggcatcg tccagtgctt tggcaatctg
     1081 gcgaacgttt tccagcgaat actgatgacg aatggcgtgc ataccgtcac gcaacgtgac
     1141 gtccgatata taaagttttt taccgttcat gccgcttctc ctgccttgcg cgccagtgac
     1201 tgggccattt tttccgctgt cgccagcgca ctggaagtca taatgtcgag gttgcccgca
     1261 taggcaggca gataatgcgc tgcgccttcg acttccagcc agaccgctgt tttcagtccg
     1321 gagaattgcc ccacgcccgg taaattgacc ggtttatcct gcgggataac ttcaaactgc
     1381 acgcgctgtt tcaggcgata tcccggtacg taagcctgca ccgcctcagc catttcattg
     1441 attgaggctt cgatatcatc ttgtgaagct tcgtcgctca atacatacac cgtgtcacgc
     1501 atcatcaacg gtggctctgc tgggttaagc acgataattg ccttcccttt tgccgcgccg
     1561 cccaccactt caatggctcg ggaagtggtt tccgtaaatt catcgatatt ggcacgcgtg
     1621 ccaggtccgg cagatttact ggcgatagaa gcgataattt cggcgtaatg aacacgcgcc
     1681 acgcgtgaaa ctgccgccac cattggaatg gtggcctggc cgccgcaggt gaccatattg
     1741 acgttcagtt gttcgacatt cgcatcaagg ttaaccaccg gcacgcagta agggccgatg
     1801 gcagcaggcg tcaggtcgat taagcgaata tccggtttcg cttcgcgcaa agcggcatcg
     1861 tttttcacat gtgcgcccgc gctggtcgca tcaaatacaa tgtcgatatc agcaaattca
     1921 ggcatgttca tcagcccgat caccccttca tgggtggtgg cgacgcccat acgtctggca
     1981 cgcgccagac cgtcggactg aggatcaata ccgaccatca ccgccatctc cagatgctga
     2041 ccgtgacgca aaattttaat catcagatcg gtgccaatgt tgccagaacc gataatggcg
     2101 actttacgct tactcatgac agacttcctt ttggggctgc gcttgaaaat gtcgcagcaa
     2161 ctgaacctat gccttcaata tgggcttcaa aacgatcgcc cgcattcact gccaccatcg
     2221 gacctaatgc cccggtaaga atgatatctc cggcgcgcag cggttcaccc agactggcca
     2281 ttttgcgtgc cagccagacg gcggcattaa gcggatgtcc caggcattcg ctgccgcgcc
     2341 cgctggaaac ctcttcgtta ttacgcgtca tcttcatggc gcagtttttc aggtctaacc
     2401 ccgccggacg ttgcgccgga ccgccgatga catacacccc gcaggaagcg ttatctgcca
     2461 cggtatcgac aaactgaatc gaccagtcgc gaatgcggct acccaccacc tccagcgccg
     2521 gaagtaccca ttcaatggcg ttatacaatt cgtcgaaggt gatatcggtt gcgggcaaat
     2581 cgcggttcaa caccagtgcg atctccgctt caatgcgggg ttgcagaaca cgggaaaaag
     2641 gaatgatttc gttatcgcca taacacatgt cggcaaataa tgtcccgaaa tccggttgat
     2701 caacgcccag ttgttgctgc actttcggat gtgtcaggcc cactttacgc cccaccacgc
     2761 gacgcccctg cgcaacgtca tattgcacat ttatgtgctg aatggcgtag gccgcttcag
     2821 cgttatcgat accaatcaga tcgcgcagcg gcgcaatcgc ttcgccctgc tctgcggcgc
     2881 ggcgtaaatc cgccgccagt tgctcaagag tatgcttcgt catgaccgtt ccttaagcac
     2941 gtgcgaggaa attcagcacc agttgattga aagcgtcggc atgttcccac tgcgcccaat
     3001 gaccacagtc gcggaagata tgcagttcag aaccggcaat gccggacagc agacgcagac
     3061 ccgcatccat cggcacaaag cggtcgttgc gcccccagac aatcagggtt tgcgctttga
     3121 tttccgccag acgtgggcca aaatccggga actgtttcgg attagcttcc aagctcttaa
     3181 cgaagttttc caggtgatcg cggcgcgaca gcatattatt caggcgcgct tcaaacaggg
     3241 cgtcggtcaa atcgctggta tcaaaaacga agatatccat catcagcttc aggttttcga
     3301 tagtcggctg acgataaagc tgattcagtc gcttaatacc ttcggttggc atcggcgtaa
     3361 acaaactcat gccgcccgta ccgccgccca tcagcaccag tttgccgacg cgctccggcc
     3421 agttaagggt gaacgccaca gaactatggc cacccatcga gttgcccagc aggtggattt
     3481 tggcgatatc cagttgatcc accacgcttt tcaggattcg tgcattaaga tccgatcgcg
     3541 aaccactatt aacgatcgaa tcgctcttgc cccaacccgg acaatccagc aggatcaccc
     3601 gatagcccgc ctctaccagc ggatcgatat tgcggctgaa gttcgcccag ccagtagcac
     3661 ccgggccgga accatgcagc aggacaacgg tttcgtcgcc ttgtccgcag tcattaaaat
     3721 ggatgcgcag cgttttaccc gcttcttcta cattcagaaa acggctggtg gcggcttcgg
     3781 tttgtggctg ataactcata atcttctcct gcatattcag ttctctgttc tggcgcttaa
     3841 cgagccaaat ccggcaatcc actccgggat tgggcggtaa taacgccctt cgctacgcca
     3901 gttgccaaat gcagaaatag cggcaaaagc ggcgacccag gttttgattt catgtgtcga
     3961 ctttccggca atggcggaaa gctcttcgtt actgacggca tccagttcct gtatgcgtcc
     4021 ctgctccagc aaagtcatga actggttatc ccaaatcggg ttgagcggat gcagagttct
     4081 ctgatcctca acaaacttct cagcggcgct aatcaccctt tgctgacgca attcgcgctc
     4141 actggcgggt aaatctttcc cgctccccaa cagacggtcg cgcatatggg catcggcttt
     4201 cgccagttcg ggcaccggcg gctgatggga aagcccaccg gaacccagga acagcacgcg
     4261 tttattgaga gtgctggtga aacgtccaat ggcttcaccc aacatgcggg tacgctggaa
     4321 accgggcagc ggcgtggcga caccgttgat gaacacaggc agaactggca ccttatccag
     4381 cccaccgagc aggaactcca gcggctgtgc gaacccgtgg tccacctgca tacagtaaga
     4441 aacggcaaga tcgatcccgc tcttcatgac ggcatgcgca caggcctccg ccagctccac
     4501 aggcactggc agctctcctg ccgcactgcc gaaatcacca attgccgtcg ctccaacgcc
     4561 taaacagaac ggtggcatca cgtcatagaa aaagccgttg tagtgatctg gcgcaaacag
     4621 caccaccagt tcaggggaga aagccgcaat acgctcgcgg gcgctggcaa tcacgccatt
     4681 gacctcatcg agtacctctt gtgctgggtc gacatacccc accagcggcg agtgggaaag
     4741 acaatgaaga taagcgtgca tatcaggcta ccttttcgac agaaacgtcg gcatcagggc
     4801 gggtcagcgt catcaccgac gccagtttat tcagggtctt gcccagggtt tgcggaatgg
     4861 cggtggcggc aacaaagcga tccgggcgca tcaccaccag cgaagcattg tgttgcgcga
     4921 accagctacg caggcgacct tgcgtatccc ccacgcgtag tacgccgtcg tggttatcct
     4981 gtgcggtatg aatttgcact tccggcacca cctgaatgaa gcgtgtgccc aacgcgcgcc
     5041 actgctggat ttgctcgtcg ctcatccccc acagtggatt gcatccccag ccaattaccg
     5101 cgaagttcgc gccgatcgcg ttatcgagta gcgtcacgtc gccgttttcc agcgtgactt
     5161 tcggctgaat aaacatcttg ccgaccggag agtgcttcgc ttcgccctca cgtaccagcg
     5221 caccgccgta atattgcggc atcggcttga agcgcatttc gaggaagtag cgttttactg
     5281 gcggcagata attcagcagc caggaaacgc cgtcacgtaa cgtaccctgc cagcgtttcg
     5341 gcggagccag cacgttgccc gccgtcacgg acaggtcaat catcgctttg gcgtgatcgc
     5401 gacgttcttg ttgataggta tcgagcagcg catcgcgggc tttcccctgg ataaccaacg
     5461 ccagtttcca tgcgaggtta aaagcgtcgc gcatgccact gttatagccc tgcccctgcc
     5521 ataccggcat gatgtgcgcg gcatcgcccg ccagcagtac gcgatcaata cggaaacgtt
     5581 gcgccagtcg cgcgttgtgg gtgtagacac gctggcgaat caattcaaca ttgtccggat
     5641 taggcagcac tttgcttaac agcttgcgca tattttgcgg ctcacgcagc tgttcttcgg
     5701 tttctcctgg catcaccata aattcaaagc gacgtaccgc atgaggtagc gcggcagaaa
     5761 catacgggcg caccggatcg caacacaaat agatatgcgg cgtacttaac ggatcgttgg
     5821 cgatatctac cacaatccac tgatttggcg cagttttacc ttcaaacggc acattcaggg
     5881 tgcgacggac aaaacttgcc ccaccatcac aggccaccag ccactgggct ttgactattt
     5941 cccgctgccc ttctgccgtt ttcaggtgca aggtcacttc gtcatcttgc tgactgaagg
     6001 cctccagctc gcgggaaaac aagcagcgca cattcggaaa acgcgacacc ccttccagca
     6061 tcaccgcatc gacctgcggc tgaataaagg cgttacggcg cggccagcca aattcatcgg
     6121 tcattggctg aatatcagca aaacagcggc ccttcggggt gagaaaacgc atcgcgtgcc
     6181 acggcgtagt atgcggcaga acattatcga ccaggccgac cgactgcatg gtgcgcagcg
     6241 cctcgtcatc aataccaatc gcacgcgggt agtcgatcaa cttatcgagt ttctccacca
     6301 ccagcacgtc aatgcccatc tggccgagat agttcgccat catcagcccg accggaccgg
     6361 caccagcgat cgccacctga acgctatggt taacagcagg ctggatgtca gggtgttgta
     6421 ttgccatttc agtacctcac gactcggaca aaatgccgtt gcgcgcacag tacagcgcaa
     6481 cttattttgt taaaaacatg taaatgattt tttattgtgc gctcagtata gaaagggtgt
     6541 tttcggctac aatcaaaaca tgcccgaatg tgcaccaggt gcacaacgtt gttttaacta
     6601 tagaaatgtc aattaatatg cagaacaatg agcagacgga atacaaaacc gtgcgcggct
     6661 taacccgcgg tctaatgtta ttaaatatgt taaataaact tgacggcggt gccagcgtcg
     6721 ggctgctggc ggaactcagc ggcctgcatc gcaccactgt gcggcgactg ctggagacgc
     6781 tgcaggaaga gggatatgtc cggcgtagtc cctccgatga tagttttcgt ctgaccatca
     6841 aagtgcggca attaagcgaa ggatttcgtg acgaacagtg gatttctgca ctggcggccc
     6901 cactgctggg cgatctgttg cgcgaagtgg tatggccgac agatgtgtcc acgctggatg
     6961 ttgatgcaat ggtggtacgc gaaaccaccc accgtttcag ccgcttatcc tttcaccggg
     7021 caatggtcgg gcgacgtttg ccgcttctga aaaccgcctc gggcctgacc tggctggcct
     7081 tttgcccgga acaagaacgc aaggaattaa tcgaaatgtt agcctcccgc cccggtgatg
     7141 actatcaact ggcacgagaa ccgttaaagc tgcaagccat tctggcgcgt gcgcgcaaag
     7201 agggttacgg acagaactac cgcggctggg atcaggagga gaagatcgcc tctatcgccg
     7261 taccgctgcg cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat atggcgagcg
     7321 caatgaccat cgaacaggca gcggaaaagc atcttccggc actacaacgg gtagcaaaac
     7381 agatcgaaga aggggttgaa tcgcaggcta ttctggtggc cggacggcga agcggcgtgc
     7441 atttacgcta attacgccaa tgtgtaattt ggcccattct catcgttatt cgcccatttg
     7501 cccacatgca cagtacgggc tgcacgctac gctaatgaca tcaatactgt gatgtggagc
     7561 aaatcatgaa aattacgcaa attcgtaatg ccacccaaat tatccaatac gctggcaaaa
     7621 catttcttat cgacccgtta ctcgcgccta aaggaagtta tccgggtttt gccggaacgg
     7681 cacgcgcaga aatccgcaat ccaacggtag aattaccgtg cgatttaaca acgttactca
     7741 acgttgacgc cctaatcgta acccatttgc atgaagatca ctgggacgaa acagcctccc
     7801 gcgtcgtccc taaagataaa ctgatttatg tgcaaaatga gcaagatgct gaatcactga
     7861 ataaacaagg atttacgcat cttaccgtac ttaccgacga gactaaattt ggcgatatta
     7921 ctctgcataa aacgacaggg cagcacggct ccgatcgcac ctatgccgac ccggctatgg
     7981 cgaagcgttt aggcgcagcc tgcggcgttg ttttccagca cccgacagag aaaacgctct
     8041 atctggctgg cgataccctc tggcgtgacg acgtcgaggt tacaatgcta actttccaac
     8101 cagaagtggt tgtgttgaat gccggatttg cgcatgttct ggggcatggc gcgataatta
     8161 tgggagcaga agatattctc aaaacacatt ttaccctgcc agaagcgcaa attgtcgcga
     8221 cacatatgga ggccgtaaac cactgtttgc tgacgcgtga tgcgctcaag gaatacgcca
     8281 gagataatca aattatcgaa tttgtgaacg taccagaaga tggtgaaacg ttgacattct
     8341 aattcggaga taaagagtgt tatgcgcccg ctaactgttg ccattatcgc cgttgccggt
     8401 tttagtcctt ttcacctttc cgtaccgttt atcgtattta gcgaaaaaat ggcggagaaa
     8461 aaacactttc acgtaattat ctgcgctgaa aagccgggaa acgtggactc tgccgatggg
     8521 ttttccgtaa ccgccaccca tgactatacc gcagtcatcc aggcggatat tgtgataatt
     8581 ccttactggg gaaccattac acaaaaaccg ccacaaaaac tgctggaagc cttaacgacc
     8641 gcacgggata acggcgcaca aattgtcggg ctttgcctgg gcacgtttgt gctcggctat
     8701 gcaggtttac tgaaaaataa gcgtgccgcc acgcactggg agttcgagcg tgaatttcag
     8761 gcacgttttc cacaaacaca tctggatatt aacgcgttgt acgtagacga tgacggcatt
     8821 attacctctg ccggtactgc cgcggcgctg gattgctgtt tgtatattgt tcggcaacat
     8881 tttggcagcg actatgctaa ccatattgcc cgacggatgg tcgtaccgcc atatcgcacc
     8941 ggcggtcagg cgcagtttat tgagcaacca gtgccgaaaa atacccatga tgaacgcatt
     9001 aatctcctgc tggattacct gcggcaaaac attgcgcaac agcatgatct cgactcgctg
     9061 gcgcagcgag taatgatgag tcgccgcaca ttaactcgcc attttatgaa agcgaccggt
     9121 tcgagtatcg ccgaatggct cattactgaa cgcttacgcc gtagccagga actgttggga
     9181 tccagtcagt tgcccgttga gcggatagcg gctgaggtgg gttttctctc acctgtgacc
     9241 tggcgtcagc attttaaatc tcacttcggc gtcagccccg ccgaatggcg caaaaccttt
     9301 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca
     9361 gtaacgctat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg
     9421 gtgaatcagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
     9481 gagctgaatt acattcccaa ccgcgtggca caacaactgg cggggaaaca gtcgttgctg
     9541 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt
     9601 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc
     9661 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
     9721 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cgctaatgtt
     9781 ccggcgttat ttcttgatgt ctctgaccag actcccatca acagtattat tttctcccat
     9841 gaagacggta cgcgactggg cgtggagtat ctggtcgcat tgggtcacca gcaaatcgcg
     9901 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggcggg ctggcataaa
     9961 tatctcactc gcaatcaaat tcagccgata gcggaacggg agggcgactg gagtgccatg
    10021 tccggttttc aacaaaccat gcaaatgcta aatgagggca tcgttcccac tgcgatgctg
    10081 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggttgcgc
    10141 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc gtgttatatc
    10201 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
    10261 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
    10321 gtgaaaagaa aaaccaccct ggcgcccaag acgcaaaccg cttctccccg cgcgttggcc
    10381 gattcattaa tgcagctggc acgacaagtt tcccgactgg aaagcgggca gtgagcgcaa
    10441 cgcaattaat gtgagtcagc tcactcatta ggcacccctg gctttacact ctatgcgtcc
    10501 ggctcgtatg ttgtgtgaaa ttgtgagcgg ataacaattt cacacaggat acagctatga
    10561 ctatgattac ggattcactg gccgtcgtat tacaacgtcg tgactgggaa aaccctggcg
    10621 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
    10681 aggcccgcac caatcgccct tcccaacagt tgcgcagcct gaatggtgag tggcaatttg
    10741 tctggttttc ggcaccagaa gcggttccgg aaagctggct ggagtgcgat cttcctgtcg
    10801 ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgacgcg cccatctaca
    10861 ccaacgtgac ctatcccatt acggtcaatc cgccatttgt tcccacggag aatccgacgg
    10921 gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa
    10981 ttatttttga tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt
    11041 acggccagga cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag
    11101 aaaaccgcct cgcggtgatg gtgctgcgct ggagtgacgg cagttatctg gaagatcagg
    11161 atatgtggcg gatgagcggc attttccgtg acgtctcgtt gctgcacaaa ccgaccacac
    11221 aaatcagcga tttccatgtt gccactcgct ttaatgatga tttcagccgc gcggttctgg
    11281 aggcagaagt tcagatgtac ggcgagctgc gcgatgagct gcgggtgacg gtttctttgt
    11341 ggcagggtga aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg
    11401 agcgtggcgg ttatgccgat cgcgtcacac taggtctgaa cgtcgaaaac ccgaaactgt
    11461 ggagcgccga aatcccgaat atctatcgtg cggtggttga actgcacacc gccgacggca
    11521 cgctgattga agcagaagcc tgcgatgtcg gtttccgcga ggtacggatt gaaaatggcc
    11581 tgctgctgct gaacggcaag ccgttgctga ttcgcggcgt taaccgtcac gagcatcatc
    11641 ctctgcgtgg tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc
    11701 agaacaactt taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacaccc
    11761 tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg
    11821 tgccaatgaa tcgtctgacc gatgatccgc gctggctacc cgcgatgagc gaacgcgtaa
    11881 cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg
    11941 agtcaggcca cggcgctaat cacgacgcac tctatcgctg gattaaatct gtcgatccat
    12001 cccgcccggt gcagtatgaa ggcggcggag ccgacacctc cgcaaccgat attatttgcc
    12061 cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca
    12121 aaaaatggct ttcgttgcct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg
    12181 cgatgggtaa cagtcttggc ggcttcgcta aatactggca ggcgtttcgt cagtaccccc
    12241 gtttacaggg cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa
    12301 atggcaatcc gtggtcggct tacggcggtg attttggcga tacgccgaat gatcgccagt
    12361 tctgcatgaa cggtctggtc tttgccgacc gcacgccgca tccggcgctg acggaagcaa
    12421 aacaccagca gcagtttttc cagttccgtt tatccgggcg aaccatcgaa gtgaccagcg
    12481 aatacctgtt ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggca
    12541 agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa caggtaattg
    12601 aactgcctga actaccgcag ccggagagca ccgggcaact ctggctaacg gtacacgtag
    12661 tgcaaccgaa cgcgaccgca tggtcagaag ccggacacat cagcgcctgg cagcagtggc
    12721 gtctggcgga aaacctcagc gtgacactcc cctccgcgcc ccacgccatc ccgcatctga
    12781 ccaccagcga aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc
    12841 agtcaggctt tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc
    12901 gcgatcagtt cacccgcgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca
    12961 ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcgg
    13021 cgttgttgca gtgcacggca gatacacttg ccgacgcggt gctgattacg accgctcacg
    13081 cgtggcagca tcagggaaaa accttattta tcagccggaa aacctaccgg attgatggta
    13141 gaggtcaaat ggcgattacc gttgatgttg aagtagcgag cgatacaccg catccggcac
    13201 ggattggcct gacctgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag
    13261 ggccgcaaga aaactatccc gaccgcctta ctgctgcctg ttttgaccgc tgggatctgc
    13321 cattgccaga catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga
    13381 cacgcgaatt gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc
    13441 gctacagcca acaacaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag
    13501 gcacatggct gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc
    13561 cgtcagtgtc ggcggaattc cagcttagcg ccggtcgcta ccattaccag ttggtctggt
    13621 gccaaaaata ataataaccg ggcaggccat gtctgcccgt atttcgcgta aggaaatcca
    13681 ttatgtacta tttaaaaaac acaaactttt ggatgttcgg tttattcttt ttcttttact
    13741 tttttatcat gggagcctac ttcccgtttt tcccgatttg gctacatgac atcaaccata
    13801 tcagcaaaag tgatacgggt attatttttg ccgctatttc tctgttctcg ctattattcc
    13861 aaccgctgtt tggtctgctt tctgacaaac tcgggctgcg caaatacctg ctgtggatta
    13921 ttaccggcat gttagtgatg tttgcgccgt tctttatttt tatcttcggg ccactgttac
    13981 aatacaacat tttagtagga tcgattgttg gtggtattta tctaggcttt tgttttaacg
    14041 ccggtgcgcc cgcagtagag gcatttatcg agaaagtcag ccgtcgcagt aatttcgaat
    14101 ttggtcgcgc gcggatgttt ggctgtgttg gctgggcgct gtgtgcctcg attgtcggca
    14161 tcatgttcac catcaataat cagtttgttt tctggctggg ttctggctgt gcactcatcc
    14221 tcgccgtttt actctttttc gccaaaacgg atgcgccctc ttctgccacg gttgccaatg
    14281 cggtaggtgc caaccattcg gcatttagcc ttaagctggc gctggaactg ttcagacagc
    14341 caaaactgtg gtttttgtca ctgtatgtta ttggcgtttc ctgcacctac gatgtttttg
    14401 accaacagtt tgctaatttc tttacttcgt tctttgctac cggtgaacag ggtacgcggg
    14461 tatttggcta cgtaacgaca atgggcgaat tacttaacgc ctcgattatg ttctttgcgc
    14521 cactgatcat taatcgcatc ggtgggaaaa acgccctgct gctggctggc actattatgt
    14581 ctgtacgtat tattggctca tcgttcgcca cctcagcgct ggaagtagtt attctgaaaa
    14641 cgctgcatat gtttgaagta ccgttcctgc tggtaggctg ctttaaatat attaccagcc
    14701 agtttgaagt gcgtttttca gcgacgattt atctggtctg tttctgcttc tttaagcaac
    14761 tggcgatgat ttttatgtct gtactggcgg gcaatatgta tgaaagcatc ggtttccagg
    14821 gcgcttatct ggtgctgggt ctggtggcgc tgggcttcac cttaatttcc gtgttcacgc
    14881 ttagcggccc cggcccgctt tccctgctgc gtcgtcaggt aaatgaagtc gcttaagcaa
    14941 tcaatgtcgg atgcggcgcg agcgccttat ccgaccaaca tatcaggacg gaatgagcga
    15001 aatgaacatg tcgatgacag aaagaataaa agcaggcaag ctatttaccg atatgtgcga
    15061 aggcttacct gaaaaaagac ttcgtgggaa aacattaatg tatgagttta atcactcgca
    15121 tccatccgac gttgaaaaaa gagaaagcct gattaaagaa atgtttgcca cggtagggga
    15181 aaacgcctgg gtagagcccc ccgtctattt ctcttgcggt tccaacatcc atataggccg
    15241 caatttttat gcaaatttca atttaaccat tgtcgatgac tacacggtaa caatcggtga
    15301 taacgtactg attgcaccca acgttactct ttccgttacg ggacaccctg tacaccatga
    15361 attgagaaaa aacggcgaga tgtactcttt tccgataacg attggcaata acgtctggat
    15421 cggaagtcat gtggtcatta atccaggcgt caccatcggg gataattctg ttattggcgc
    15481 gggtagtgtc gtcataaaag acattccacc aaacgtcgtg gctgctggcg ttccttgccg
    15541 ggttattcgc gaaataaacg accgggataa gcaatattat ttcagagatt acaaagttga
    15601 atcgtcagtt taaattagca aaattgcctg atgcgcttcg cttatcaggc ctacaaattc
    15661 agtgttctac gttagccgca tccggcatga acaaagcgca ggaacaagcg tcgcatcatg
    15721 cctctttgac ccacagctgc ggaaaacgtg ctggtgcaaa acgcagggtt atgatcatca
    15781 gcccaacgac gcacagcgca tgaaatgccc agtccatcag gtaattgccg ctgatactac
    15841 gcagcacacc agaaaaccac ggggcaagcc cggcgatgat aaaaccgatc ccctgcataa
    15901 acgccaccag cttgccagca atagccggtt gcgcagagtg atcgagcgcc agcagcaaac
    15961 agagcggaaa cgcgccgccc agacctaacc cacacaccat cgcccacaat accggcaatt
    16021 gcagcggcag ccagataaag ccgcagaaac ccaccagttg taacgccagt gccaacagca
    16081 aaagtttgcg ccgatcctga tggcgagcca tagcaggcat tagcaaagct cctgcggctt
    16141 gcccaagcgt catcaatgcc agcaaggaac cgctgtactg cgcgctggca ccaatctcaa
    16201 tatagaaagc gggtaaccag gcaatcaggc tggcgtaacc accgttaatc agaccgaagt
    16261 aaacacccag cgtccacgcg cgaggagtga aaaccacgcg aacctgagtg gttgttgtct
    16321 tgtgggaaga ggcgacctcg cgggtgcttt gccaccacca ggcaaagagc gcaacaacgg
    16381 caggcagcgc ccaccaggcg agtgtttgat accaggtttc gctatgttga actaaccagg
    16441 gcgtcagggc ggcaccaagt ccaccgccgc ccatcagagc cgcggaccac agccccatca
    16501 ccagtggcgt gcgctgctga aaccgccgtt taatcaccga aggcatcacc gcctgaatga
    16561 tgccgatccc caccccacca agcagtgcgc tgctaagcag cagcgcactt tgggggtaaa
    16621 gctcacgcat caatgcaccg acggcaatca gtaacaaact gatggcgacg ctgcgacgtt
    16681 cgctgacatg ctgatgaagc cagcttccgg ccagcgccag cccgcccatg gtgaccaccg
    16741 gcagagcggt caacagggca gccacgctaa agctcattcc gcttgcctgg cgcaattgcg
    16801 gtagcagtgg cccgacggag gtgagcagtg gtcgcatatt aagaccaatc agcaccagaa
    16861 ccagcagcat cgtgaacgtc ttttgatggt tacgcatggc tgtcagaacg gtttggtcgg
    16921 cagatattta ccatctaagg tgatgaccgc acgttcgcca ccttccgggt ccgccacttt
    16981 cttaacgtcg agtttgaagt taatcgcgct gataatgcca tcgccaaact tctcatgaac
    17041 caacgctttc agggttgtgc cgtacacctg caacatttca tagaaacgat acatcgttgg
    17101 gtcggttgga atacggtcat caatgcagcc acgcagtggg atcatctgta acagtagaat
    17161 ggcgtcttcg tcgagatcca gcttcgcccc gacctggcgg gcggcgtcgg caggaagcgc
    17221 ctgctgaccc agcaaagccg cggtcacaaa ggcttctgcc agcccggtgc cgtcggcaat
    17281 ctcggcaaat gagagatctt ttttagcttt gctgagcaaa atggcatcgg caagatcaag
    17341 acgaatattg cgattaattt gcgactgaat catggtgcat ctcctgatgg tttaaaaata
    17401 aggttacgct gcggtcggtt ggcgtagcgg tatggcacaa acgcgaggat tagcggccag
    17461 tggcacaaac tggcgggttg cgccgtcaaa cgctgcgatg ctgccgcttt caatgtcgta
    17521 gacccagccg tgcagggcga tccgcccctc ttcgagcgcc aggcgcaccg atggatgagt
    17581 ttgcaaatta gccaactgag caatgacgtt ttcacgtacc atcgccgcag cttttgacgg
    17641 taaatcggaa tgcgggcgcg cctcattaac gacgcgggct gaatcggcat aacgcagcca
    17701 gtgggagacg gcaggcatat ggtccatgca ctgacagctg gcaatggcgg tcatcgcgcc
    17761 acagttggaa tgaccgcaaa tcacaatgtc agatacccga agcgcagcga cggcatactc
    17821 caccgaagca gaaacgccac cgggttccgg cccgtaggaa gggacgatat tgcccgcgtt
    17881 gcgaataacg aacagatcgc caggctcacg ttgcgtcacc agctcaggga ccagacggct
    17941 gtcggagcag gagataaaaa gtgtgcgtgg gctttgctgt gtcgccaact gtttaaataa
    18001 ggcttcccgc ttcggaaatg cctcgcgctg gaatttaagg aatccatcaa taatctcttt
    18061 caccgttaac ctctgtctgt ctctggaatg agaggcagac tactcgccga ttgtcataag
    18121 gtaaaagtct catttatgat gagttctatt ggatttactt ataggttggc gaatgctctc
    18181 tcgacatatc aattattttc ttgccgtggc tgaacatggc agcttcaccc gtgccgccag
    18241 tgcgttgcac gtctctcagc ctgcgctttc ccagcagatt cgccagttag aggagagttt
    18301 aggcgtgccg ctgtttgacc gtagcgggcg aacgattcgt ctcactgatg caggagaagt
    18361 ctggcgacag tacgccagcc gggcgttaca ggaactggga gcgggtaaac gggcgattca
    18421 tgatgttgcc gatctgacga gaggatcgct gcgtatcgcc gtcaccccca cctttacgag
    18481 ctactttatc ggccccttaa tggcggattt ctatgcgcgc taccccggca tcacgctcca
    18541 gctacaggaa atgtcgcagg agaaaatcga ggatatgctt tgccgcgacg agttggacgt
    18601 tgggattgcc ttcgcgcctg tgcattcgcc ggagctggag gcaattcctt tactgacaga
    18661 aagtttagcg ttagtcgtgg cgcaacatca tccgctggcc gtccatgaac aggtggcgtt
    18721 gagtcgcttg catgatgaaa aactggtcct gctcagcgcg gagtttgcca ccagagagca
    18781 aattgaccac tactgcgaga aagcggggct gcatccacag gtggtcattg aggcgaactc
    18841 aattagcgcg gttctggagc tgattcgccg cacttccctt tccacattgt taccagcagc
    18901 gattgccaca caacatgacg ggcttaaagc tatttctctt gccccgccac tactggagag
    18961 aacggcggtt ttgttgcggc ggaaaaatag ctggcagaca gccgccgcga aggcattttt
    19021 gcacacggcg ttggaggaat gtgcggatgt tggcgagaat gaatcacggt agccactggc
    19081 acaggtctgc ggctggaatt gcctgatgcg ctacgcttat caggcctacg agttcagtgt
    19141 aaggcgttca cgccgcatcc atcataaact acagctggca gacagccgcc gcgaaatcat
    19201 ttttgcacat ggcattggaa gaatgcgcgg atgttggcgg gaatgaatca cggtagccgc
    19261 tgacacagat cagcggctgg aattgcctga tgcgctacgc ttatcaggcc tacgagttct
    19321 acgagttcag tgctctacgt aggccggata aggcgttcac gccgcatccg gcataaacta
    19381 cgctcgcttt gtcaacacac agcccagccg atcaccgttt gtaatcgatg gcttctggct
    19441 gctccagata tacggtggtt tgtgccggtt gtgtgctggc aatcaccttg ccgccacgta
    19501 ccgaataacg taccggaacc tgacggcgca gcgcatcaaa tccattttca gccggcagga
    19561 taatcaggtt ggcgctgttt ccggcggcaa tgccgtaatc ctgcaaattc aacgtcctgg
    19621 cgctgtggtg ggtgattaaa ttcaggccat cgttaatctg cccatagccc atcagctggc
    19681 aaacatgcag ccccatatgc agcacttgca gcatattcgc cgttcccagc ggataccacg
    19741 gatcgaagac atcatcgtga ccaaagcaga cgttaatgcc ggactccagc atctctttaa
    19801 cgcgcgtgat gccgcgacgt tttggatacg tatcgaaacg tccttgcaga tgaatattga
    19861 ccagcgggtt ggcgacaaag ttaataccgg acattttcag caagcggaac aggcgtgagg
    19921 tatacgcccc gttataggag tgcattgccg tggtgtggct ggcggtgact cgcgcgccca
    19981 tgccttcacg gtgcgccagg gcagcaacgg tttcgacaaa gcgcgactgc tcgtcatcga
    20041 tctcatcaca gtgaacgtcg atgagacggt cgtatttttg cgccaaggcg aaggttttat
    20101 gcagcgactc cacgccgtat tcacgggtaa attcaaaatg cggaatcgcc cccactacat
    20161 ctgcccctaa gcgtaacgcc tcttccagca acgcttcacc gttgggatac gacaaaatcc
    20221 cttcctgagg gaaggcgacg atttgcagat caatccacgg cgcgacttcc tgcttcactt
    20281 ccagcattgc tttcagcgca gttagcgttg catccgaaac atcgacatgg gtacgcacat
    20341 gctgaatgcc gttggcaatc tgccatttca gcgtttgcca tgagcgttgt ttcacatcgt
    20401 catgggttaa taacgctttg cgctcggccc agcgttcaat gccttcaaac agcgtgccgg
    20461 actgattcca gttcggttgt ccggcggttt gcgtggtgtc caggtgaata tgtggctcca
    20521 caaacggcgg tataactaaa ccttgttcgg catccaggct gttttcagtt atgggcatca
    20581 cgccggattg cgcatcaatg gcgcagattt ttccgtcctg cagatgaatc tgccacagcc
    20641 cctctttgcc tggtaaccgg gcgttaataa ttgtttgtaa agcgttattc gacactgtta
    20701 gcctccacat gcgtcattgc tgctgtcgtt ttacgattca aaatcgggtt aaggatcaga
    20761 tagctcagcg cgccacctaa taccgcgttg accggaacaa ttcccggtaa ccagtggcct
    20821 gcagcaatcc ccagggcgac cgccagaatc gccacccaat tgacactcat catacgcgtg
    20881 gtcgcaaagt gctcatagcg gcgacggttc atcagatagt cggcaatgat cacgccaccc
    20941 actggaggaa tagctgccga aaggaaggtc aaccagccga caaaattgtt atacagccat
    21001 aatgcacaga ccgtaccgat aataccgttg attaccgaaa gggttttgct cgacatcccg
    21061 gtaatgttgg cgaaacctaa acccgacgca tagagcgcgt tatcgttggt ggtccagata
    21121 tttagcccca gcaccacaat cgcaggcaac agcaggccct gagcaatcat cacatcagag
    21181 atatccgcca accccagtgc cgccgctccc gctgcaccga aaataaacat caacgagttg
    21241 ccgaggaaaa aggccaccat cgccaccagc accgccagtt tggcattgcg accaaagcgg
    21301 acaaagtcag cggtgagcgt acccgcgctg ataaatgacc ccacaaccag cgccagcgcg
    21361 acattgaaat ctaatggttg tgcgggaacg accgctttta aggcgtccag gccgcccatg
    21421 ccgttaacgg ccagccacac ggaataaccg ccaagacagg cgatcgccgg aaccgcaatc
    21481 accgaaagaa ctgtcagcgc cgaaatgcca aagaagacgg tgacggtcat cagtaaaccg
    21541 gaaacggcaa tcagcaaatt aatatccagc ccggttgcct tacccaccgg aatggcaaac
    21601 atcgccacgc caacgccaaa ccagccaacc tgggtgccgc ccagcagcag tgaaggcagc
    21661 catgagcctt taacaccaaa cgagaagcga gcaagaagat gagtggtcag gccggttttt
    21721 gcgccaatgt aaccgagaaa tgaagtgtaa ataccgagga gaagattgcc gatgagaact
    21781 gcgaggaaga aatcatgata gctaagaccg gttccgagag tgccgccagt ccacatactg
    21841 gcggaaaaga aggttaatcc cagcatgacg aacgtcaatg ccaatacccc tttccgcgcc
    21901 gactgcggga ctggcccctg gctaaagttg ttatcttgcg acacgaaatt cccccttttt
    21961 gctgtttgaa acccaaaaaa tccgccgcat tctattcatc tgaagataaa aagcaatcgt
    22021 tttcgtgggg aaatatattt tttatatgaa tgagtgcaaa aaggtggggg aaaaattatc
    22081 ggtgtaaaaa cagtgggtag gcctgataag acgcgtcagc gtcgcatcag gcaactgcgc
    22141 atggtgtcgg atgcggcgcg agcgccttat ccgacctacg gttcaggcct gtaggcatga
    22201 taagacgcgc ctgcgtcgca tcaggcatcg gcgcatggtg tcgaatgcgg cgcgagcgcc
    22261 ttatccgacc tacggttcag gcctgtaggc atgataagac gcgcctgcgt cgcatcaggc
    22321 atcggcacac ggtgtcggat gcggcgcgag cgccttatcc gacctacggt tcaggcctgt
    22381 aggcatgata agacgcgtca gcgtcgcatc aggcatctgc
//
LOCUS       DAZFKF010000079        22272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFKF010000079 DAZFKF010000000
VERSION     DAZFKF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.079"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1171)
                     /gene="ydcO"
                     /locus_tag="ACHKA1_003585"
     CDS             complement(<1..1171)
                     /gene="ydcO"
                     /locus_tag="ACHKA1_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO5681182.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQMLAGLALLSTISG
                     SLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNR"
     gene            1263..1799
                     /gene="sutR"
                     /locus_tag="ACHKA1_003586"
     CDS             1263..1799
                     /gene="sutR"
                     /locus_tag="ACHKA1_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO5681183.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVIISLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1872..3833
                     /gene="rlhA"
                     /locus_tag="ACHKA1_003587"
     CDS             1872..3833
                     /gene="rlhA"
                     /locus_tag="ACHKA1_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO5681184.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTDPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3925..4155)
                     /gene="yncJ"
                     /locus_tag="ACHKA1_003588"
     CDS             complement(3925..4155)
                     /gene="yncJ"
                     /locus_tag="ACHKA1_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO5681185.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4377..4553
                     /gene="hicA"
                     /locus_tag="ACHKA1_003589"
     CDS             4377..4553
                     /gene="hicA"
                     /locus_tag="ACHKA1_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
                     /protein_id="HFO5681186.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKVRFHGMHSVMPRHPCDEIK
                     EPLRKAIQKQLGLS"
     gene            4599..5015
                     /gene="hicB"
                     /locus_tag="ACHKA1_003590"
     CDS             4599..5015
                     /gene="hicB"
                     /locus_tag="ACHKA1_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO5681187.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5094..6500
                     /gene="ydcR"
                     /locus_tag="ACHKA1_003591"
     CDS             5094..6500
                     /gene="ydcR"
                     /locus_tag="ACHKA1_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO5681188.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQVVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            6745..7890
                     /gene="ydcS"
                     /locus_tag="ACHKA1_003592"
     CDS             6745..7890
                     /gene="ydcS"
                     /locus_tag="ACHKA1_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO5681189.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVKQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7908..8921
                     /gene="ydcT"
                     /locus_tag="ACHKA1_003593"
     CDS             7908..8921
                     /gene="ydcT"
                     /locus_tag="ACHKA1_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5681190.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVLVSWSRDVMVPLV
                     EER"
     gene            8922..9863
                     /gene="ydcU"
                     /locus_tag="ACHKA1_003594"
     CDS             8922..9863
                     /gene="ydcU"
                     /locus_tag="ACHKA1_003594"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5681191.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPIAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            9853..10647
                     /gene="ydcV"
                     /locus_tag="ACHKA1_003595"
     CDS             9853..10647
                     /gene="ydcV"
                     /locus_tag="ACHKA1_003595"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5681192.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10669..12093
                     /gene="patD"
                     /locus_tag="ACHKA1_003596"
     CDS             10669..12093
                     /gene="patD"
                     /locus_tag="ACHKA1_003596"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO5681193.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(12190..12285)
                     /gene="yncL"
                     /locus_tag="ACHKA1_003597"
     CDS             complement(12190..12285)
                     /gene="yncL"
                     /locus_tag="ACHKA1_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO5681194.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            12480..12653
                     /gene="ortT"
                     /locus_tag="ACHKA1_003598"
     CDS             12480..12653
                     /gene="ortT"
                     /locus_tag="ACHKA1_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO5681195.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            12739..12972
                     /gene="ydcY"
                     /locus_tag="ACHKA1_003599"
     CDS             12739..12972
                     /gene="ydcY"
                     /locus_tag="ACHKA1_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO5681196.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(12973..13422)
                     /gene="ydcZ"
                     /locus_tag="ACHKA1_003600"
     CDS             complement(12973..13422)
                     /gene="ydcZ"
                     /locus_tag="ACHKA1_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5681197.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(13419..13937)
                     /gene="mddA"
                     /locus_tag="ACHKA1_003601"
     CDS             complement(13419..13937)
                     /gene="mddA"
                     /locus_tag="ACHKA1_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO5681198.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            14118..15155
                     /gene="curA"
                     /locus_tag="ACHKA1_003602"
     CDS             14118..15155
                     /gene="curA"
                     /locus_tag="ACHKA1_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO5681199.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVEESNHPDYQSGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKIFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            15353..16018
                     /gene="mcbR"
                     /locus_tag="ACHKA1_003603"
     CDS             15353..16018
                     /gene="mcbR"
                     /locus_tag="ACHKA1_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO5681200.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(16054..18156)
                     /gene="pqqU"
                     /locus_tag="ACHKA1_003604"
     CDS             complement(16054..18156)
                     /gene="pqqU"
                     /locus_tag="ACHKA1_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO5681201.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLIGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            18398..19459
                     /gene="yncE"
                     /locus_tag="ACHKA1_003605"
     CDS             18398..19459
                     /gene="yncE"
                     /locus_tag="ACHKA1_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO5681202.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISVI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(19574..21073)
                     /gene="ansP"
                     /locus_tag="ACHKA1_003606"
     CDS             complement(19574..21073)
                     /gene="ansP"
                     /locus_tag="ACHKA1_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO5681203.1"
                     /translation="MSKHNTDTSEQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            21341..21958
                     /gene="yncG"
                     /locus_tag="ACHKA1_003607"
     CDS             21341..21958
                     /gene="yncG"
                     /locus_tag="ACHKA1_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO5681204.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPTLQEILKRNEII"
     gene            22034..22246
                     /gene="yncH"
                     /locus_tag="ACHKA1_003608"
     CDS             22034..22246
                     /gene="yncH"
                     /locus_tag="ACHKA1_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HFO5681205.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVKMFHLLSQAFYNLK
                     MFLMMDMLGVGDTININTNKNIRQVC"
BASE COUNT         5671 a         5472 c         5712 g         5417 t
ORIGIN      
        1 atcggtttct gtcagcgatt aaattcaaca ccacgtaaca aacgcctccg gcaattaatc
       61 cccagaacgc agaaccaatc ccgaccagcg tcaatccact tgccgttacc agaaatgcca
      121 ccaccgccgc gtctcgctca cgctcattat gcagcgcctg atacaaactg ccgctaatgg
      181 tacttaacag cgccagacct gccagcatct ggatccaact tacgggcagg gcagccatca
      241 tcccggtaat agcactacca aacagacctg cgatcaaata aaaaatgcct gcaacggcag
      301 ctgccagcca acgttgatct ttatccggat gcgcttccgg gctttggcaa atagccgcgg
      361 tgattgccgc aataccgacg gaataaacgc cgaaagggga aaaaaccagt gccagcaatc
      421 cagtaaatac aattaatggc gaaacaggag ccgaataccc ggctgctttc attgctgcga
      481 tacccggtgc gttttgcgat gccatcgtca ccagaaaaag ggggagtgca acgctcaggc
      541 tgtgagcaaa cgaaaaatca ggggtaatat aagtggggag aacgggttta aagacaacat
      601 cagttgtgac aacgtcacct tgcgcgataa cgatcacgat cccaataatc atcgcggcaa
      661 ttaccgcata gcgcggcgca acggccttgg ttgccagcca taccagcaac atacttccac
      721 acaacgtaaa ttgaccgtcc agactggcaa acgcctgtaa accaaagcgt aataaaatcc
      781 cggcaagcat tgccgccgca agcgagtgcg gaataatgcg catcagacga gcaaagagtc
      841 ccgttatgcc gcagaggact attaacgcgt tggtgacaat aaaaacgccg atggcttcgt
      901 taagtgttag tccctgcaat ccggtgacca acaaagccgc gccaggcgtt gaccatgcgg
      961 tgagaacagg tacgcgatac cataatgtca gagtcagcgt actgacgccc attgccagcc
     1021 ccagcgccgt catccagcca gagatttgtg cagtggtggc tccggcgaca atcgctgctt
     1081 gccagattat tgccgctgaa ctggcgtagc caattaatac cgccagaaac cccgccagta
     1141 gcgtgggtgg aggaatagaa aacagacgca tagtcacctc gtgcgttata gcgtacaggc
     1201 accgtagcat ttgtccgtta taacgcacaa gtgataaact tccgttttgc cggaggagtc
     1261 gcatggaaaa tctcgctcgc tttttatcca ccacacttaa acaactacgt cagcagcgcg
     1321 gctggagtct ttcgcgactg gcagaagcga cgggcgtttc taaagcaatg ttaggacaga
     1381 ttgagcgtaa tgagtccagc ccgacggtcg cgacattatg gaaaattgcc actgggttga
     1441 acgtgccatt ttcgacattt atttctccgc cacagtccgc tacgccttca gtttatgatc
     1501 cacaacagca ggcgatggtg atcatttcgc tgtttcctta tgatccacaa ctctgctttg
     1561 aacacttctc gattcagatg gcacccggtg caataagcga atcgacgccc catgagaagg
     1621 gggtaatcga acatgtcgtc gtcattgatg gacaacttga tctgtgcgtt gatggtgaat
     1681 ggcagaccct taattgtggg gaaggcgttc gatttgctgc agacgtcacg catatctatc
     1741 gtaatggtgg ggagcaaacc gtacattttc attccctcat ccattacccg cgcagttaag
     1801 cgggaaaact atttcgcaac gtcgcgcttg tggctaaaat agccgccatt tttcagctac
     1861 tggataagaa tgtgaccgta tcttctcatc gacttgaact gttaagcccg gcacgcgatg
     1921 ccgccattgc ccgcgaagct attttgcacg gtgccgatgc tgtttatatc ggcggccctg
     1981 gttttggtgc ccgtcataat gccagtaata gcctgaaaga tattgccgag ctggtgccgt
     2041 ttgcccatcg ttatggtgca aaaattttcg tcacgcttaa caccattttg catgatgatg
     2101 agctggaacc cgcgcaacgg ctgattactg acctctacca gaccggtgtc gatgcgctga
     2161 ttgttcagga tatggggatt ctggaacttg atattccgcc gattgaactg cacgccagta
     2221 cgcagtgcga cattcgtaca gtagaaaaag cgaagttcct ttctgatgtt ggcttcacgc
     2281 agattgtgct ggcgcgagag ctgaatcttg atcagatccg cgcgattcac caggccacgg
     2341 acgcgaccat tgaattcttt attcatggcg cactgtgcgt ggcctattcg ggtcagtgct
     2401 acatttctca tgcgcaaaca gggcgtagcg ccaaccgtgg cgattgctcg caggcgtgcc
     2461 gtttgccata cacattgaaa gacgatcagg ggcgggtggt ttcctatgaa aaacatctgc
     2521 tgtcgatgaa agataacgat cagactgcca acctcggcgc gctgattgat gctggcgtac
     2581 gctccttcaa gattgaaggg cgttacaaag atatgagcta cgtgaagaat atcaccgccc
     2641 attatcgcca gatgctggat gccattattg aagaacgtgg cgatctgacg cgcgcttcat
     2701 caggtcgtac tgaacatttc tttgttccat cgacggaaaa gactttccac cgtggtagca
     2761 cagattattt tgtgaatgcc cgtaaaggcg atattggcgc gttcgattcg ccgaaattta
     2821 tcggcctgcc ggtgggtgaa gtattgaaag tggcgaaaga tcatctcgat gttgccgtta
     2881 ccgatccact ggcaaatggc gatggcctga acgtgttgat taaacgtgaa gtcgtcggtt
     2941 ttcgtgccaa tacggtcgag aaaaccggag aaaatcagta ccgcgtctgg cccaatgaaa
     3001 tgccagcaga tttgcacaaa attcgcccgc atcacccact aaaccgtaat cttgatcata
     3061 actggcagca ggcactgaca aaaacctcca gcgaacgtcg ggtggcggta gacattgaac
     3121 tgggcggctg gcaggaacaa ctgattctga ccctcaccag tgaagagggg gtcagcatca
     3181 cgcatacgct ggatgggcag ttcgacgaag ccaataacgc cgaaaaagca atgaacaatc
     3241 tgaaggatgg tctggcaaaa ctggggcaaa ccatctatta cgcccgcgat gtgcaaatta
     3301 atttgccggg ggcgctgttt gtaccaaaca gtctgttaaa ccagttccgc cgtgaagctg
     3361 ctgacatgct ggatgctgcg cgtcttgcca gttaccagcg cggcagccgt aaaccggttg
     3421 ctgatcctgc gccggtttat cctcaaacac atctgagttt cctcgcgaac gtttacaacc
     3481 agaaagcgcg tgaattttat catcgctatg gtgtgcagct gattgacgcg gcgtatgagg
     3541 cacatgaaga gaagggcgaa gtcccggtga tgatcaccaa gcattgtctg cgctttgcct
     3601 ttaatctgtg cccgaaacag gcgaaaggca atatcaaaag ctggaaggcg acgccaatgc
     3661 aactggtaaa cggcgatgaa gtattaacgc taaagtttga ttgccgccca tgcgagatgc
     3721 acgtcattgg caaaatcaaa aatcacatac tgaaaatgcc gttaccggga agcgtagtgg
     3781 catccgtaag tccggatgag ctgctgaaaa cattgccgaa gcgaaaaggg taaaacgcca
     3841 gttttctggt tactcacaac ttattgaatc tgcatgatat tgcctgccgg gtaaggcgtt
     3901 acgccgcatc cggcatcaaa cgactcaacg ttgtcccgct tctggtttgc gcgatttttg
     3961 ccagtaaaaa tgctcgcgca aaccttccgc cgactcttcc gccacagcac gcaattcatc
     4021 gctgtccgct tcatgacgca gctgatgatc cacattcttt acccacacaa attcatgtcc
     4081 tttgtgccct gccatgagtt gtcctgagaa cagcgcacac gttaataaga caaccgataa
     4141 cgccttcgta aacatcctgc cacctttttc ttaccttttg ccgctatgat gccgatcgtt
     4201 tcttgaggtt attattcagt tttgcaaatt agcgcaaaga aattctggaa tcttccttcc
     4261 tgattttgca ttgcattctg ccgttgcggc aatttagtgc tattttcgag caaattacac
     4321 acggaggtaa acactaatgt ttactttgtt gatatactca gcggcaggga ggcgatgtga
     4381 aacaaagcga gttcagacgt tggctcgaat ctcagggcgt cgatgtagcg aatggcagca
     4441 accatttgaa agtcaggttt catgggatgc acagtgtcat gccgcgtcac ccctgcgatg
     4501 agattaaaga accattgcgt aaagcaatcc agaaacaact cggtttgagt taatcgccaa
     4561 ttaaaaaggt taatgacatg cgagagacag tcgaaattat gcgttatccc gtcactctta
     4621 cacccgcgcc ggaaggcggt tatatggttt cttttgtgga tatccctgaa gcgttgaccc
     4681 agggcgaaac tgtcgctgaa gcgatggaag cggcaaaaga tgctttactg accgcatttg
     4741 atttttattt tgaagataac gagcttatcc cgttaccttc gccattaaat agtcacgatc
     4801 attttattga agtacctttg agcgtcgcct ctaaggtatt gctgttaaat gcttttttac
     4861 agtcagaaat cactcagcaa gagttagcca ggcgaattgg caaacctaaa caggagatta
     4921 ctcgcctatt taacttgcat catgcgacaa aaatcgacgc cgtccagctc gcggcaaagg
     4981 cgcttggcaa agagttatcg ctggtgatgg tttaattaca gttaacgaaa agttgtcatt
     5041 tttaacaact gatatagact gccgaatcat ctgcacataa ttacgattcg ataatgaaaa
     5101 aataccagca gcttgcagaa caattacgcg agcagattgc gtcgggtatc tggcaacccg
     5161 gcgatcgttt accttcgttg cgtgaccagg tggcgctttc tggcatgagc tttatgactg
     5221 tcagccatgc ctatcagttg ctcgaaagtc agggatatat tatcgcacga ccgcagtcgg
     5281 gttattacgt tgcgccacag gcaataaaaa tgccgaaagc gccagtcatt ccagtcactc
     5341 gagatgaagc agtcgatatc aacacttata tttttgatat gttgcaggcc agtcgcgatc
     5401 cgtcggtcgt tccgtttgcc tcggcctttc ccgacccgcg acttttcccc ctccaacaac
     5461 taaaccgctc gctggcgcag gtaagcaaaa ccgccacggc gatgagtgtg attgaaaact
     5521 taccgccagg aaacgcagaa ctgcgtcagg ctattgctcg tcgctatgcc ttacagggca
     5581 tcaccatttc tcctgatgaa attgtcatta ctgccggggc gttagaggca ttaaacctca
     5641 gtttgcaagt ggtaactgaa ccgggcgatt gggtgatagt agagaatcct tgtttctacg
     5701 gtgcgttgca ggcgctggag cggctacggc tgaaggcgtt atcggtggcg acggatgtta
     5761 aagaagggat cgatcttcag gcgctggaac tggcgttgca ggagtatccg gtgaaagcgt
     5821 gctggctgat gactaatagc cagaatcctc tcggatttac cttaacgccg caaaaaaaag
     5881 cacaactggt ggcgttgctc aatcagtaca acgtaacgct gattgaagat gacgtttaca
     5941 gcgaacttta ttttggacgg gaaaaaccgc tgcctgcgaa agcgtgggat cgccacgatg
     6001 gcgttttgca ttgctcttcg ttttcgaaat gtctggtgcc tggttttcgt attggttggg
     6061 tcgccgccgg aaaacatgca cgtaaaattc aacgcttgca gttgatgagt acgctttcca
     6121 ccagctcacc gatgcagctt gcgctggtgg attacctttc cacgcgccga tacgacgccc
     6181 atcttcgtcg cctgcgtcgc cagcttgcgg aacgtaaaca acgtgcctgg caggcactgc
     6241 tgcgttacct gcctgcggaa gtgaaaattc atcataatga cagcggttat tttctctggc
     6301 tggagctccc cgagccgtta gatgccggcg aattgagcct ggcggcactg acgcatcata
     6361 tcagtattgc gccgggtaaa atgttttcta ccggtgaaaa ctggtcacgt ttttttcgtt
     6421 ttaataccgc gtggcagtgg ggagaacgtg aagaacaggc ggtaaaacaa ttaggcaaac
     6481 ttattcaaga acggctgtaa tagcgtttaa tttaattcct cttagattgg gtaatatgaa
     6541 tttcgaatag cagtcatatt tcctaactcc ttgtctatac tccagaagat aaccttacag
     6601 acggcataat gcgcggtagc tcacaacctg attaaatttt ctcaggggcg aaggtgtgcc
     6661 tgcaagccgc cgtctatggt taaacaagga gatattttta cggcacggcg gctgaacaat
     6721 taattacgac aggagtaaga ccttatgagc aagacatttg cccgcagcag cctgtgtgcg
     6781 ctcagcatga caataatgac cgctcacgcc gccgaacccc ctaccaattt agataaaccg
     6841 gaagggcgac tggatattat cgcctggccg ggatatatcg aacgcggaca aactgataaa
     6901 caatacgact gggtaaagca attcgaaaaa gagacaggct gcgcggtgaa tgtgaaaact
     6961 gcggcgactt ccgatgaaat ggtcagcctg atgaccaaag gaggttacga tctggtcacg
     7021 gcgtccggcg atgcctcgct gcgtttgatt atgggtaaac gcgtgcagcc gattaatacc
     7081 gccttgattc ccaactggaa aacgctcgat ccgcgcgtgg ttaaaggcga ctggtttaac
     7141 gttggcggca aagtttacgg cacaccttac caatgggggc cgaacctgct gatgtacaac
     7201 actaaaacct tcccgacgcc gccggatagc tggcaagtgg tttttgttga gcaaaatctg
     7261 ccggacggca agagcaataa aggccgcgtt caggcttatg atggccctat ctatattgcg
     7321 gacgctgcgt tgttcgttaa agccactcag ccgcagttgg gcatcagcga tccgtatcaa
     7381 ctcaccgaag aacagtacca ggcggtgctg aaagtgctgc gcgctcaaca cagtttgatc
     7441 catcgctact ggcatgacac taccgtgcaa atgagcgatt tcaaaaacga gggtgtggtc
     7501 gcttccagtg cctggcccta tcaggccaac gccctgaaag ccgaaggcca gcctgttgct
     7561 accgttttcc cgaaggaggg tgttaccggt tgggctgata ccaccatgct gcatagcgaa
     7621 gcgaaacatc cggtttgcgc ctacaaatgg atgaactggt cattaacgcc aaaagtgcag
     7681 ggcgatgtgg cggcctggtt tggctcgtta ccggtagtgc cggaagggtg taaagccagt
     7741 ccgttattag gcgaaaaagg ttgtgaaacc aacggtttta actatttcga caaaatcgcc
     7801 ttctggaaaa cgcctatagc agaagggggc aagtttgttc cctacagtcg ctggacgcag
     7861 gattacattg ccattatggg cggtcgctaa cttcgctggg gtgctttatg acgtacgcag
     7921 tggagtttga caacgtctcg cggttgtacg gtgacgtgcg cgcagtagat ggcgtcagta
     7981 ttgcgataaa agatggtgag ttcttctcta tgctggggcc gtccggctcc ggcaaaacca
     8041 cctgcctgcg cctgattgct ggcttcgaac agctttccgg cggggctatc tctatctttg
     8101 gtaaacccgc cagcaatctg ccaccgtggg agcgggacgt gaatactgtc tttcaggact
     8161 acgcgctatt tccgcatatg tcgattcttg acaatgtcgc ctatgggctg atggtcaaag
     8221 gcgtgaataa aaagcagcgg cacgcaatgg cgcaagaggc gctggagaaa gtggcgttgg
     8281 ggtttgtaca gcaacgtaaa ccgtcacaac tttctggtgg tcagcgccag cgggttgcta
     8341 tcgccagagc attggtgaat gaaccgcgcg tattgctgtt ggatgaaccg ctcggcgcac
     8401 tggatctcaa attgcgtgag cagatgcagc tggaactgaa aaaactgcaa cagtctctcg
     8461 gtatcacttt tatcttcgtt acccacgatc agggcgaagc gttatcgatg tccgatcgtg
     8521 tggcggtttt caataatgga cgcattgagc aggtcgattc cccgcgcgat ctctatatgc
     8581 gcccgcgcac gccgtttgtt gccgggttcg ttggtacatc gaatgttttt gatggactga
     8641 tggcagagaa actttgtggc atgacgggaa gcttcgccct gcggccggaa catatccgcc
     8701 tcaacacccc tggtgaactg caggccaatg gcacgatcca ggcggtgcaa tatcagggcg
     8761 cggcaactcg ttttgaactg aaattgaacg gcggtgaaaa actgctggtg agtcaggcca
     8821 atatgacagg cgaagaactg cctgccacgc tcacgcccgg acaacaggtg ctggtttcct
     8881 ggtcgcgtga tgtgatggtg ccgctggttg aggagaggtg aatggcgatg aacgtattgc
     8941 aatcaccttc acgtccaggt ctgggtaagg tgtccggttt cttctggcgg aatccggggc
     9001 tggggctgtt tttgctgctg cttggcccgc taatgtggtt tggcattgtc tatttcggct
     9061 cgctgctaac gctgttatgg cagggctttt atacctttga cgatttcacc atgtcggtaa
     9121 cgccggaact gacgctggcg aacatccgtg cgctgtttaa tccggcgaat tacgacatca
     9181 ttctccgcac gctgactatg gctgtggcag tcactatcgc cagcgccatt ctggcttttc
     9241 caatagcgtg gtatatggcg cgctatacca gcgggaaaat gaaagcgttt ttttatattg
     9301 cggtaatgtt gccgatgtgg gcgagctaca ttgttaaagc ctatgcctgg acgttattgc
     9361 tggcaaaaga tggcgtggct cagtggtttt tacaacatct tgggctggaa ccactgctga
     9421 cagcgttcct tacattacct gcggtgggcg gcaatacgct gtcaacttcc gggctggggc
     9481 gctttctggt gtttctctat atctggctgc cgttcatgat cctgcccgtg caggcggcgc
     9541 ttgagcgttt gccgccgtca ttgttgcagg cgtcagctga tctcggcgca cgtccacgac
     9601 aaacctttcg ctatgtggtg ttgccgctgg cgatcccggg tattgccgct ggctctatct
     9661 ttaccttctc actcacactg ggcgatttta tcgtcccgca gctggttggc ccgccaggat
     9721 attttatcgg caatatggtt tattcccagc agggggcgat tggcaatatg ccgatggcgg
     9781 cggcattcac cctggtgccg attgttctta tcgcactgta cctggcgttc gtgaaacgtc
     9841 tgggagcgtt cgatgcactc tgaacgcgca ccgtttttcc tcaaactggc ggcctggggc
     9901 ggcgttgttt tcctacattt tcccatcctg ataatcgccg cctatgcgtt taacactgaa
     9961 gatgcggcgt ttagttttcc accgcagggc ctgacgctgc gctggtttag cgtggcagca
    10021 cagcgtagtg atattcttga tgcagtgaca ctgtcactta aagtggcggc gctggcgaca
    10081 ttaatcgcgc tggtgttagg gacgctggca gctgccgcgc tgtggcgacg agactttttc
    10141 ggcaaaaacg ccatttcgct gttactgctg ctgcccattg cgctgccggg cattgtcact
    10201 ggtctggcgt tattaaccgc ctttaaaacc atcaatctgg agccgggatt tttcaccatc
    10261 gtggtcggtc atgcgacttt ttgtgtagtt gtggtgttta acaatgtcat cgcccgtttt
    10321 cgccgcacct catggagtct ggttgaggcg tcaatggatc ttggggccaa tggctggcaa
    10381 accttccgct acgtagtgtt gccgaatctc agttcggcgt tactggcagg aggaatgctg
    10441 gcgtttgcct tgtcgttcga tgaaatcatc gttacgacct ttacggcagg tcatgaacga
    10501 acgttaccgt tgtggttgct caatcagctt gggcgaccgc gtgatgtacc ggtaaccaac
    10561 gtggtggcac tgctggttat gttggtaaca accttgccga tcctgggggc ctggtggcta
    10621 acccgcgaag gcgacaatgg tcaataacca ctgatacagg aaaatgctat gcaacataag
    10681 ttactgatta acggagaact ggttagcggc gaaggggaaa aacagcctgt ctataatccg
    10741 gcaacggggg acgttttact ggagattgcc gaggcatccg cagagcaggt cgatgctgct
    10801 gtgcgcgcgg cagatgcagc atttgccgaa tgggggcaaa ccacgccgaa agtgcgtgcg
    10861 gaatgtctgc tgaaactggc tgatgttatc gaagaaaatg gtcaggtttt tgccgaactg
    10921 gagtcccgta attgtggcaa accgctgcat agtgcgttca atgatgaaat cccggcgatt
    10981 gtcgatgttt ttcgcttttt cgcgggtgcg gcgcgctgtc tgaatggtct ggcggcaggt
    11041 gaatatcttg aaggtcatac ttcgatgatc cgtcgcgatc cgttgggggt cgtggcttct
    11101 atcgcgccgt ggaattatcc gctgatgatg gcagcgtgga aactggctcc ggcgctggcg
    11161 gcagggaact gcgtagtgct taaaccatca gaaattaccc cgctgaccgc gttgaagttg
    11221 gcagagctgg cgaaagatat cttcccggca ggcgtgatta acgtactgtt tggcagaggc
    11281 aaaacggtgg gcgatccgct gaccggacat cctaaagtgc ggatggtgtc gctgacgggc
    11341 tctatcgcca ccggcgagca catcatcagc cataccgcgc cgtccattaa gcgtactcat
    11401 atggaacttg gtggcaaagc gccagtgatt gtttttgatg atgcggatat tgaagcagtg
    11461 gtcgaaggtg tacgtacatt tggctattac aatgccggac aggattgtac tgcggcttgt
    11521 cggatctacg cgcaaaaagg catttacgat acgctggtgg aaaaactggg tgctgcggtg
    11581 gcaacgttaa aatctggtgc gccggatgac gagtctacgg agcttggacc tttaagctcg
    11641 ctggcgcatc tcgaacgcgt cagcaaggca gtagaagagg cgaaagcgac agggcacatc
    11701 aaagtgatca ctggcggtga aaagcgcaag ggtaatggct attactatgc gccgacgctg
    11761 ctggctggcg cattacagga cgatgccatc gtgcaaaaag aggtatttgg tccagtagtg
    11821 agtgttacgc tcttcgacaa cgaagaacag gtggtgaact gggcgaatga cagccagtac
    11881 ggacttgcat cttcggtatg gacgaaagat gtgggcaggg cgcatcgcgt cagcgcacgg
    11941 ctgcaatatg gttgtacctg ggtcaatacc catttcatgc tggtaagtga aatgccgcac
    12001 ggtgggcaga aactttctgg ttacggcaag gatatgtcac tttatgggct ggaggattac
    12061 accgtcgtcc gccacgtcat ggttaaacat taaggataat attgcagatc gtaagagtat
    12121 gagatgatct tacgtactgt cagagcactg atttctctga cagtacgtgc tgcgtgcaaa
    12181 atcactacat caaataaacc agccaaatct catagagata agagtaaaca aaccaacagc
    12241 agcaaagaca tttatcagta ctacagttct actggaaaca ttcatttttg ccacctttta
    12301 tcattgcccc tatgtgtata gtacgggtta agaaaatccg caataatact cattctgaag
    12361 atggataagg gcaagttgct gtttgatgat tttttctcaa actggtgaaa aaacttgatg
    12421 cacgtcaaaa aatgacgcat atttgcgcgc gttttattca tctggctgga cgcccgtaca
    12481 tgtctctcta tcaacacatg cttgtcttct atgcggttat ggcagcaatc gcatttctta
    12541 tcacctggtt tctttctcac gataagaaac gcatccgttt cttaagcgcc tttctggtgg
    12601 gggcaacatg gccgatgagt ttcccggtgg cgctgttgtt ttcactgttt taaatcttac
    12661 tcactggctt gacggcacca cgcagactta tatcatttgg atgaatcgat aaatttcaca
    12721 agtggctaag gagaaagtat gtcgcatctg gatgaagtca tcgcgcgcgt ggatgccgcg
    12781 attgaagaga gcgtcattgc ccatatgaac gaattattaa tcgccctgag cgatgacgcg
    12841 gagttaagtc gggaagatcg ctacacccag cagcagcgtt tgcgcaccgc tattgctcat
    12901 cacggtcgca agcataaaga agatatggaa gcgcgccatg aacagttaac caaaggcggc
    12961 accatcctgt gattaaaatg agcgtctggc taccaaccag gcgccaacca caagtaaaat
    13021 agcgccacaa attgggccga acaacgcccg tagtggtacg ccatgactgc ggaagatatc
    13081 cagaaccaga ccgccaatca gctggctggc gaccagtact gcaatagttg tcgccgctcc
    13141 cacattctga tatccgctga tactggcgaa gacaaaaaac gatcccagca agccaggtat
    13201 aagtgtccac cagcgcacgc tggacaccag ttcgccaaac cccgccatac cttgtttaaa
    13261 ccataaaata gaaacaaaga gcacaatccc caccagtgag ttcagcaaca tggcgatgag
    13321 aatggtagag gaggtctggg tgatgcgcac catcagcgtg ttctggacta ccagaccaat
    13381 tccggcggca atcaaaaagg caagggtgag cgactgattc atccaatcgc gtccggttca
    13441 gtgcgttcgt cgagttgcaa ctgcataaat gtcagatcca gccagcgccc aaatttagtg
    13501 cccacctgcg gcatttgtgc ggtgacgaca aatcccagcg actggtgcag atgcagcgag
    13561 gcctgatttt gcgattcgat cccggcgacc atgacatgct tcccgcaatc tcgcgcttca
    13621 tcaatcaatc ggcttaacaa tttacgcccc agacctttgc cctgatgatc gggatggaca
    13681 taaaccgaat gttccacggt atggcgaaaa ccatcgaaac tacgccagtc gccaaatgag
    13741 gcatatcccg tcactacgcc atcttcttca cttaccagca ccggataacc tgctatggtc
    13801 cgcgcttcaa accaggcaat gcggttatct gcatccaccg tttggtcatt ccagatagcc
    13861 gccgtataca acacggcgtg gttataaatt tccgcaatgg cagcacagtc ggctttgcgg
    13921 gcaaaacgga tggacatgtc tggcctcaat aaaataatga tgatgatgtt atcaatacta
    13981 tcgaaaacag cccgaagcaa cggattcctc gatgaaatta ttgcgtgttg cagagggggt
    14041 gtgacggctg caaacaaaac caatcctgcg agcctgctac tattgatgga ttcatgccaa
    14101 aagcacagga gagcattatg gggcaacaaa agcagcgtaa tcgacgttgg gttctggcct
    14161 cgcgtccaca tggcgcaccg gttccggaga atttccgtct tgaagaagat gatgtcgcca
    14221 caccgggtga aggacaggtg ttactgcgca cagtttattt gtccctggac ccgtatatgc
    14281 gtggacgtat gagcgatgag ccatcttatt caccgccggt tgatattggc ggcgtgatgg
    14341 tcggcggtac ggtgagccgt gtcgaggagt cgaatcatcc tgattatcag tctggcgact
    14401 gggtgctggg ctacagtgga tggcaagact atgacatatc cagtggtgat gatctggtga
    14461 aacttggcga tcatccgcaa aatccatcgt ggtcgctggg tgtgctaggg atgccaggct
    14521 ttaccgctta tatgggccta ctggatatcg gtcagcctaa agagggcgaa acgttggtgg
    14581 tagctgcggc gacaggacca gtgggggcga cggtggggca aatcggcaaa cttaaaggtt
    14641 gcagagtggt gggggtagcc ggtggcgcgg aaaaatgccg ccatgctacc gaggtgttag
    14701 gcttcgatgt ttgccttgat caccacgcgg acgattttgc agaacaactg gcgaaagcgt
    14761 gcccaaaagg tattgatatc tattatgaaa acgtgggcgg taagatattt gatgcagtgc
    14821 taccattact taatacatct gcgcgcattc ccgtttgtgg cttagtgagc agctataacg
    14881 ctacagagct accacccggt ccggatcgtt tacccctgtt gatggcaacg gtgctgaaaa
    14941 aacgtattcg cttgcaaggg tttattatcg ctcaggatta tggtcaccgc atccatgagt
    15001 ttcagaagga gatggggcaa tgggtgaaag aggataaaat ccactaccgc gaagaaatta
    15061 ctgacggttt agagaatgcg ccacagacgt ttatcggcct gctgaagggt aaaaacttcg
    15121 gcaaagtggt gatccgcgtg gcgggtgatg attaaagttt caacggcggc gtaagccgcc
    15181 ataaaaatcc aggcaaaaag cttattttca gctttaatta actaaccaca tcattgttct
    15241 gctgctaata acaacaaagg tgagtggttt atgattattc actttaatac accaggtgaa
    15301 ttccttctgc catgcaggca gggttggaca gaaaacacgc gaaggaatta aaatgcctgg
    15361 aacggaaaaa atgaaacatg tcagtttgac tctgcaggtt gagaacgacc tgaaacatca
    15421 gcttagtatt ggcgcactaa aacctggcgc acgcctgatt actaaaaatc tggcggagca
    15481 attaggtatg agtattacac ctgtgcgtga agcattatta cgtctggttt cggtgaatgc
    15541 gctttctgtc gcaccagcgc aagcatttac agttccggaa gtggggaaac gtcaattaga
    15601 cgaaatcaat cggatccgct acgaactgga attaatggca gttgctctgg ctgttgaaaa
    15661 cctcaccccg caagacctgg cggaactcca ggaattgctc gagaaattac agcaggcgca
    15721 agaaaagggc gacatggaac aaatcattaa tgtaaacagg ctatttcgct tagcgattta
    15781 tcatcgctca aatatgccca tcctgtgtga gatgattgag caactgtggg tcaggatggg
    15841 acctggttta cattatctct atgaagcgat taatccagcg gaattacggg agcatataga
    15901 aaactatcat ctgttactcg ctgcgttaaa agcaaaagac aaagagggat gcagacattg
    15961 tcttgctgaa attatgcaac aaaatattgc tattttatac cagcaataca atcgttaaag
    16021 taaatgcccg gcaaataccg ggcatcaagg taattactca aatcgccatg cgatattcac
    16081 gccgacacca taatttcgtc cgggcgcagg ttcgtaatat cgcccgtttg actcattgac
    16141 aatgacagaa ccaacgtatt ctttatcgaa taaattatcg acacgaccaa ataaatccac
    16201 agttaaattg tggtaattgt atttataccc ggtgaataag ccgatgagag tataagacgg
    16261 cgccttggcc gtattttcat catctgccat aatatcgccc atataacgcg cttccgtgcc
    16321 tgcataccag ccatcttccg gtacataacc tatcgacgca aagcccatat tacgggcgat
    16381 ccctggcatc cgattaccgt tacaatcctg ttcattgcaa acattgctgc gataggtcgc
    16441 atcaagccag gtccaggacg cgtttacgcg aaaatctccc gcgaaacgtt gatcccatgc
    16501 cagttcagcg ccttgacgac gggtctttcc agcatttttg taagtcgtac gcccaccgct
    16561 actgctatcg acaacaattt catcatcggt gtcggtctgg aacaatgcga gactaagcag
    16621 cccatcacca atacgcgttt tactgccgat ctcaattgta tcgttggtgg atggttttaa
    16681 accaaagttc ataccgcttt gcccatcagc gcgataagac agctcattaa tcgtcggcgt
    16741 ttcaaaacca cgcccggctg ccagatagat attccaggca tcggtcattg catattttaa
    16801 cgatccggct ggtagccatt tgtgataact ggcatcaccg ctgtcatcgc cgttacccgg
    16861 agtaacgtaa tggtcgttgg aatcaaacca cacggagctg tagcgcacgc cagcatccag
    16921 cgacagtttt tcgctcagct gccactgcgt ctgtaaatag ggatcgatgt tccacatcag
    16981 attgcgttcg tcgcgacgca actcaccttt ttgcccgtac tccggcatgc cgctattcag
    17041 gcggaagtta ttgtagccct tgcggttttc actcatgttt tcgtagttca ggccggtagt
    17101 gaacgtgacc ggaacgccca gttcaccacg gtgtgtccag cggctgtcta ttccctggta
    17161 atggcgttgc agggtaatca cgccgcccgc atgtgacggg ttaagttgtg gcgccatggg
    17221 tattgactgg tactgggtcg tttctcgctc tccggcatac atcatcacac tcatatcatc
    17281 ccgcgaactc aggctacgtt catagcgcaa cccagcctga gtttgcttga tggtttttcg
    17341 cgtgtcgtac tgttctgcac gaggcgcttg ttgtggattc gccttccatt ctgctttggt
    17401 tagcccaccc gggtcatctg ctttgatatc cacactattg aaaatcaggc ttaatttgct
    17461 ggcttcatca atgcgtacgc ccagtttggc attggctaaa tttttctgtg cgccactatg
    17521 gtcacgatag ccgtgggtcg taaaacgcgt ggttgagacg gtgtaatcaa catcgccggg
    17581 ctgtgtgccg tctcccgttg cgcccgttgc tttcagccca tagcgccagc tgccaaaact
    17641 gccgtagtaa ctactggctt caatggttgg tggctgttgt ccggtctggg tggtgacatt
    17701 cattacaccg ccagacgcgt tgccatacag ggcagagaag gggccacgca gcacttccac
    17761 attttgcaca ctgcttaaat cgatgttgga tgtttgccct tgcccgtcgg gcatggtggc
    17821 gggaataccg tccacataca ggcgaatacc gcgaattccg taagtggaac gggagccaaa
    17881 tccgcgaatc gacagctgta aatcttgcgc atagttctgc cggttttgta cctgcaaacc
    17941 aggcacgccg gtcagtgatt cggacaagtt aatgcgcggt gttgccaggc gcatctcctc
    18001 gccatccacc acgcttactg ctgcaggggt atccagttct gaaaccacct gcggcgcggc
    18061 actgacaatc atggtctgtt catcagcggc aaaaacaacg ggggaaagga caagcagtgc
    18121 gggcaaaacg gtctgtcgga cggaaaaaat cttcatgaaa aaagccaggt taagaatggg
    18181 aaaacgccgt catggtaatg aaattgtaaa tttatggaaa atgaaacggc acaatacgtt
    18241 aagtaattga gaaaattgta gtcgtaacgg caagaaatgc tccacatttg agaaaataat
    18301 gattaccatt cccatttata acaagagcgt aacgatgatt acgcttagcg aagcattgtg
    18361 aagcagcaaa aatatcggtt catcaaaggg agtcgtcatg catttacgtc atctgttttc
    18421 atcgcgcctg cgtggttcat tactgttagg ttcattgctt gttgcttcat cattcagtac
    18481 gcaggccgca gaagaaatgc tgcgtaaagc ggtaggtaaa ggtgcctacg aaatggctta
    18541 tagccagcaa gaaaacgcgc tgtggctcgc cacttcgcaa agccgcaaac tggataaagg
    18601 tggcgtggtt tatcgtcttg atccggtcac tctggaagtg acgcaggcga tccataacga
    18661 tctcaagccg tttggtgcca ccatcaataa cacgactcag acgttgtggt ttggtaacac
    18721 cgtaaacagc gcggtcacgg cgatagatgc caaaacgggc gaagtgaaag gccgtctggt
    18781 gcttgatgat cgtaagcgca cggaagaggt gcgcccgctg caaccgcgtg agctggtagc
    18841 tgatgatgcc acgaacaccg tttacatcag tgttattggt aaagatagcg tgatttgggt
    18901 cgttgatggc gagaatatca aactgaaaac cgccatccag aacaccggta aaatgagtac
    18961 cggtctggcg ctggatagca aaggcaaacg tctttacacc actaacgctg acggcgaatt
    19021 gattaccatc gacaccgccg acaataaaat cctcagccgt aaaaagctgc tggatgacgg
    19081 caaagagcac ttctttatca acattagcct tgataccgcc aggcagcgtg catttatcac
    19141 cgattctaaa gccgcagaag tgttagtggt cgatacccgt aatggcaata ttctggcgaa
    19201 ggttgcggca ccggaatcac tggctgtgct gtttaaccca gcgcgtaatg aagcctacgt
    19261 aacgcatcgt caggcaggta aagtcagtgt gattgacgcg aaaagctata aagtggtgaa
    19321 aacgttcgat acgccgactc atccaaacag cctggcgctg tctgccgatg gcaaaacgct
    19381 gtatgtcagt gtgaaacaaa aatccactaa acagcaggaa gctacccagc cagacgatgt
    19441 gattcgtatt gcgctgtaat agatatggcg agggtgaaaa cggagagact tgcctgactt
    19501 ttatcacgcc gcatgcggca caccttcgtc agtaatctaa atttgcccgg cacgtagccg
    19561 ggcaattttg caattagctc gccgtttcag gcttaaacac aatttcctgt ttttcttcat
    19621 cttcctcgac gactggcgca gtgctgtgaa tttcagcaac gcgtttgcgc acaccaaacc
    19681 agcctataac cagcagaata ccgataatcg gcagcgccgc gatagtataa gtcccgttcg
    19741 ggtaatcgaa cgccatcagc acaaggacac tcagtaaaaa cagtaatgtc agccaggaag
    19801 tgaagggcgc gccaggcagt ttaaaactga catccgctgc tttgccttct ttaatcgctt
    19861 tacgcaggcg catctggcac acgatgataa acgcccatga agcgatgatt cccagcgacg
    19921 cgaagttcaa cacaatctca aatacgcgcg acggcaccag atagttgagg aatacgccga
    19981 cgacatacac aactagtgtc gccagaatcc cggcatacgg cacatgctga cgactcattt
    20041 tcgccataaa actcggtgcg gaaccgccca tcgccattga gcgcagaata cgtccggtgc
    20101 agtacagacc tgaattcagg ctggagaggg cagcggtcag caccacaatg ttcataatgc
    20161 tgccgatata tggcacaccc agtttagaga aaaacgtcac gaacggactt tgccccgcct
    20221 gatacgcgct ccacggcaat aacataacca gcaacaccac ggagccgacg taaaacaggc
    20281 caatacgcca aatcacactg ttaatcgctt taggcaccat ggtctgcgga tctttacatt
    20341 ctccggcagc tgtacccacc atttcaatgg aggcaaaagc aaacactacg ccctgaatca
    20401 acaccagcgc aggcagcaaa ccgtggggga agaagccgcc attatcggtg attaaatgaa
    20461 agccagtggt gttgccatcc agcggctgac cactaccgag gaacactgta cccacgacca
    20521 aaaaggtcac aatggcgagc actttaataa gcgcaaacca gaactccatc tccgcaaacc
    20581 atttcacacc gatcatattc atggtgccaa cgatggtaag tgcagcgagc gcaaagaccc
    20641 actgcggcac gccgccaaac gcaccccagt aatgcatata cagagcgacg gcggtaatat
    20701 caacaatccc ggtcatcgcc cagttgatga agtacatcca accggcaaca taagcggctt
    20761 tctcacccag aaactcacgg gcataggaga caaagctgcc actggaaggg cgatgcaata
    20821 ccagctcacc caacgcgcgc agaataaaaa acgaaaacag gccgcagatt aaatagacca
    20881 gtgccagcgc cggccccgcc atttgcagtc gggctcccgc gcctaaaaac aaaccagtac
    20941 caatcgcacc gccaatggcg atcatctgca cctgacgatt gcccattgct ttgtgatatc
    21001 cctcttcgtg ggcattaagc cagcggcgtt tcgcagcgtg ttgctctgaa gtgtcggtgt
    21061 tgtgtttact cattgcgctc cctgattgct ttaatgaaaa aagtctttta tgtcgccatg
    21121 aacaatgttt attcatcctg gcgatgatag ttatcaccat tattctttat gggtataaaa
    21181 ctatcggcgc agcatcctac ccgttatcga taaacgatgc aaaacatccc cttacaatcc
    21241 tgaaggggat taatacaact gacgaaaaaa tgacaaatcc ttttgctggt taacctgtgt
    21301 actgtcctac acttaatctt taaaagattg tgaggggcat atgattaagg tgtatggcgt
    21361 acccggatgg ggctctacaa tcagtgagct gatgctaaca ctggctgata ttccttatca
    21421 gttcgttgat gtcagtggtt ttgaccacga gggagcttca cgcgagttat tgaaaaccct
    21481 gaacccgttg tgtcaggttc cgacactggc attggaaaat gacgagatta tgacggagac
    21541 agcggcgatt gcattgatgg ttctcgatcg tcgcccggac cttgcgccac cggttgggcg
    21601 cgccgaacgt cagcagtttc aacggctatt ggtctggctg gttgctaatg tctatccaac
    21661 attcactttc gccgattacc ctgagcgttg ggctcctgac gcacccgaac agttaaagaa
    21721 gaatgttatt gaatatcgta aatcgcttta tatctggctg aattcacaat tgaccgctga
    21781 accttatgcg tttggtgagc aactaacgct ggtggactgt tatctttgca ctatgcgcac
    21841 atgggggcct gggcatgaat ggtttcagga taatgccacg aatatcagtg cgattgctga
    21901 tgctgtatgt cagttaccaa cgttacaaga aatactaaaa aggaatgaaa ttatttgagt
    21961 gggataccag aagattatgt tatttattat tcagtaactt tatccacaat gatgggcgta
    22021 attaattaga ttcatgttat gttttttaat ttatatcact ctccctttca ttcagcttgt
    22081 ctatttcatt tcctctgaaa agaaactaac tattcacatt gtgaagatgt ttcacttgtt
    22141 atcacaggca ttctataatc tcaaaatgtt tttaatgatg gatatgctcg gagttggaga
    22201 tacaattaat attaatacaa ataaaaatat ccggcaggta tgctaaaagg ataaatggcg
    22261 ttctctgtat tt
//
LOCUS       DAZFKF010000080        21697 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFKF010000080 DAZFKF010000000
VERSION     DAZFKF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.080"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..297
                     /locus_tag="ACHKA1_003609"
     CDS             <1..297
                     /locus_tag="ACHKA1_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO5681206.1"
                     /translation="GRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLG
                     GEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            667..1491
                     /gene="gadX"
                     /locus_tag="ACHKA1_003610"
     CDS             667..1491
                     /gene="gadX"
                     /locus_tag="ACHKA1_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO5681207.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMKRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQRLPNRDSAASIVAQGNFYGADCSAEGIRL"
     gene            1859..2587
                     /gene="gadW"
                     /locus_tag="ACHKA1_003611"
     CDS             1859..2587
                     /gene="gadW"
                     /locus_tag="ACHKA1_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO5681208.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNDSG
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2950..6063)
                     /gene="mdtF"
                     /locus_tag="ACHKA1_003612"
     CDS             complement(2950..6063)
                     /gene="mdtF"
                     /locus_tag="ACHKA1_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO5681209.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGADDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6088..7197)
                     /gene="mdtE"
                     /locus_tag="ACHKA1_003613"
     CDS             complement(6088..7197)
                     /gene="mdtE"
                     /locus_tag="ACHKA1_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO5681210.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7305..7583
                     /locus_tag="ACHKA1_003614"
     CDS             7305..7583
                     /locus_tag="ACHKA1_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681211.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7584..8111)
                     /gene="gadE"
                     /locus_tag="ACHKA1_003615"
     CDS             complement(7584..8111)
                     /gene="gadE"
                     /locus_tag="ACHKA1_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO5681212.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSALNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8910..9482)
                     /gene="hdeD"
                     /locus_tag="ACHKA1_003616"
     CDS             complement(8910..9482)
                     /gene="hdeD"
                     /locus_tag="ACHKA1_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO5681213.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9737..10069
                     /gene="hdeA"
                     /locus_tag="ACHKA1_003617"
     CDS             9737..10069
                     /gene="hdeA"
                     /locus_tag="ACHKA1_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO5681214.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10185..10511
                     /gene="hdeB"
                     /locus_tag="ACHKA1_003618"
     CDS             10185..10511
                     /gene="hdeB"
                     /locus_tag="ACHKA1_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO5681215.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10575..11222
                     /gene="yhiD"
                     /locus_tag="ACHKA1_003619"
     CDS             10575..11222
                     /gene="yhiD"
                     /locus_tag="ACHKA1_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO5681216.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQIRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11274..12044)
                     /locus_tag="ACHKA1_003620"
     CDS             complement(11274..12044)
                     /locus_tag="ACHKA1_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFO5681217.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCILHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(12041..13033)
                     /gene="shuU"
                     /locus_tag="ACHKA1_003621"
                     /gene_synonym="chuU"
     CDS             complement(12041..13033)
                     /gene="shuU"
                     /locus_tag="ACHKA1_003621"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFO5681218.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(13082..13705)
                     /gene="chuY"
                     /locus_tag="ACHKA1_003622"
     CDS             complement(13082..13705)
                     /gene="chuY"
                     /locus_tag="ACHKA1_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFO5681219.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRVQNQECHGFVRRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(13705..14199)
                     /gene="hutX"
                     /locus_tag="ACHKA1_003623"
     CDS             complement(13705..14199)
                     /gene="hutX"
                     /locus_tag="ACHKA1_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFO5681220.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPFSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            complement(14212..15549)
                     /gene="hutW"
                     /locus_tag="ACHKA1_003624"
     CDS             complement(14212..15549)
                     /gene="hutW"
                     /locus_tag="ACHKA1_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFO5681221.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDEAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIVREKP"
     gene            complement(15569..16483)
                     /gene="shuT"
                     /locus_tag="ACHKA1_003625"
     CDS             complement(15569..16483)
                     /gene="shuT"
                     /locus_tag="ACHKA1_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFO5681222.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQISQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVAPHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRITDVVESLHQQLWPQ"
     gene            17165..19147
                     /gene="chuA"
                     /locus_tag="ACHKA1_003626"
                     /gene_synonym="shuA"
     CDS             17165..19147
                     /gene="chuA"
                     /locus_tag="ACHKA1_003626"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFO5681223.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     IDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            19196..20224
                     /gene="chuS"
                     /locus_tag="ACHKA1_003627"
     CDS             19196..20224
                     /gene="chuS"
                     /locus_tag="ACHKA1_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001110409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFO5681224.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MHGDIREILAALESVGETKCICRNEYAVHEQVGTFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAV"
     gene            complement(20285..20815)
                     /gene="dctR"
                     /locus_tag="ACHKA1_003628"
     CDS             complement(20285..20815)
                     /gene="dctR"
                     /locus_tag="ACHKA1_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO5681225.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(20971..21537)
                     /gene="slp"
                     /locus_tag="ACHKA1_003629"
     CDS             complement(20971..21537)
                     /gene="slp"
                     /locus_tag="ACHKA1_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO5681226.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         5742 a         5056 c         5190 g         5709 t
ORIGIN      
        1 ggtcgcccgg acgaaggcat tccggcggtt tgcttcaaac tcaaagatgg tgaagatccg
       61 ggatacaccc tgtacgacct ctctgaacgt ctgcgtctgc gcggctggca ggttccggcc
      121 ttcactctcg gcggtgaagc caccgacatc gtggtgatgc gcattatgtg tcgtcgtggc
      181 ttcgaaatgg actttgctga actgttgctg gaagactaca aagcctccct gaaatatctc
      241 agcgatcacc cgaaactgca gggtattgcc cagcagaaca gctttaaaca cacctgataa
      301 cataacgttg taaaaaccga atgcccagcc tttaaaaaaa cagctgggca ttcggttgct
      361 tattaatcgc aataatatat tggctgtcta ttcatcgtgt taataagata tatgctatat
      421 gaatgtttat taaatgtagc gattaccttt aatgtcacga aaagatatta tttgcttact
      481 attaatttcc ctgcgttctt aaacacgttt tttgtattat aaatccagtc atcctgcccg
      541 tagaacatca cacattatca tcctgttctc ccgcttcgtt taaatttttt atcaatcaat
      601 ttgacttaag agggcggcgt gctacattaa taaacagtaa tatgtttatg taatattaag
      661 tcaactatgc aatcactaca tgggaattgt ttaattgcgt acgcaagaca taaatatatt
      721 ctcaccatgg ttaatggtga atatcgctat tttaatggcg gtgacctggt ttttgcggat
      781 gcaagccaaa ttcgagtaga taagtgtgtt gaaaattttg tattagtatc aagggatacg
      841 ctttcattat ttctcccgat gctcaaggag gaggcattaa atcttcatgc acataaaaaa
      901 gtttcttcat tactcgttca tcactgtagc agagatattc ctgtttttca ggaagttgcg
      961 caactatcgc agaataagaa tcttcgctat gcagaaatgc tacgtaaaag agcattaatc
     1021 tttgcgttgt tatctgtttt tcttgaggat gagcacttta taccgctgct tctgaacgtt
     1081 ttacaaccga acatgcgaac acgagtttgt acggttatca ataataatat cgcccatgag
     1141 tggacactag cccgaatcgc cagcgagctg ttgatgagtc caagtctgtt aaagaaaaaa
     1201 ttgcgcgaag aagagacatc atattcacag ttgcttactg agtgtagaat gaaacgtgct
     1261 ttgcaactta ttgttataca tggtttttca attaagcgag ttgcagtatc ctgtggatat
     1321 cacagcgtgt cgtatttcat ttacgtcttt cgaaattatt atgggatgac gcccacagag
     1381 tatcaggagc gatcggcgca gagattgccg aaccgtgact cggcggcaag tattgttgcg
     1441 caagggaatt tttacggcgc tgactgttct gcggaaggaa taagattata gagttttact
     1501 cagacataaa aaaaacccgg cataggggac cgggaagagg atagtctgcc gtctccagac
     1561 taataaaccg ttataacact ccctgttggc acgggaaact ttgtgctctc agtaagttaa
     1621 atataacttt tactggaaat aagatcagcc atttttttat aaacataagc tatacgctgt
     1681 gcgaaaatat attcgtgctg catttactta ttatcaatta actgttatgc aaaactactt
     1741 tgtggataaa ttttggtcct accaaatctg gcagtttttg cgctaagaaa cagtctggca
     1801 tcatttcatt agtatactga aattgaaata atcgcagtat gaaatataag ggataatcat
     1861 gactcatgtc tgctcggtga tcctcattcg tcgttcattc gatatttatc atgaacagca
     1921 aaaaatatcg ctgcataacg agagtatcct gctgctggag aaaaatttgg cagacgattt
     1981 tgcgttttgt tcaccggata cgcgacggct ggatatcgat gagctgacag tttgccatta
     2041 cttacaaaat attcgtcagc taccacgcaa tttagggtta catagcaaag accgtttgtt
     2101 aattaaccag tcacccccca tgccgctggt gacggcgatt tttgatagtt tcaatgactc
     2161 cggggtaaat tcgccgatac tgagcaaaat gctctatctt tcctgtttat cgatgttttc
     2221 tcataagaaa gaactgatcc ccttactttt caatagtatc agtactgttt caggaaaagt
     2281 tgaacgcctt attagctttg atatcgccaa acgttggtat ctgcgcgata tcgcggaaag
     2341 aatgtatacc agcgagagtc taatcaaaaa aaagttgcag gatgaaaata cctgtttcag
     2401 taaaatatta ctcgcttcca ggatgtcgat ggccagacga ttactcgagt tacgtcaaat
     2461 tcctctgcat actattgcgg aaaaatgtgg ctatagcagt acgtcgtact ttataaacac
     2521 atttcgacaa tattatggtg taacgccaca tcagtttgcg caacattcgc caggtacctt
     2581 ttcctgacat attttgcatt tgaatattgg tcaggatctc acacctgctt catgtgaaac
     2641 tcttccctga tgatttctgc cgggctaccg gctagttctc tttcgcagtt tgccaggcgg
     2701 tataggtatt ttcaggagga aaagactcgg catgtttggg attattaagc tgacaattca
     2761 tacgattacg ggaatgtggg tcagtattgt gctgttcaaa ttgatgacga atggctggag
     2821 tggtttctac tttcagtgct gtgtactaag cctggtattc ctgacggtta gctggttgtt
     2881 gagtggtgaa tggttagcag gaaagagtaa ggctgaacct tcacgttcaa ccttactctc
     2941 atttacacgt tacgcttttt taaagcgggc aaagagatgt tccactacaa cgaaaaagac
     3001 cggaacaaag taaattgcca gcactgttgc ggcaaacatc ccgcccatca cgccggtacc
     3061 taccgcgttt tgcgcgccag aaccggcacc atgactgata accagcggca gcacgccaag
     3121 aataaaggcc agagaggtca tcaggattgg gcgtaaacgc attcgtgccg cttcgatgat
     3181 tgcctctatc ggcgttttcc cttctttctg catcatctca acggcaaatt cgacaatcag
     3241 gatggcgttc ttggcggaaa gcccgatggt ggtcagcaaa ccaacctgga agtagacgtc
     3301 attacttaag ccgcgcagat cggtggccag taatgcgcca acgacgccta acggaacaac
     3361 caacatcacc gagaacggaa ttgaccagct ctcataaagt gcggcgaggg cgaggaacac
     3421 cacgaccagt gaaatggcat atagcgcggg agcctgattt gaggataacg cttcctgata
     3481 cgacagtccg gtccatgagt agccgacgcc tgccggaagt ttagcgacca ggtctgccat
     3541 aaatttcatt gcgtcaccgg tacttttccc ggccgccgct tcacctaaaa tctccattga
     3601 cgggatgccg ttgtagcgtt ccagtcgcgg tgaaccatag gtccattcgg tagacgagta
     3661 ggcagaaagc ggtgccatcg tgccagaggc gttgcgtaca taccattggt tgatgttatc
     3721 cggcaacata cggaacggcg tgcctgcctg gacatacact tttttcaccc gcccctggtt
     3781 gaggaagtcg ttcacgtagc tgctgccgaa ggcggtggaa attgtctggt tgatatcaga
     3841 cagcgccaca cccattgcct cagctttcgc ggcgttgacg ttcactttga acatcggcgt
     3901 atcttccagg ccgttcgggc gtaccccggt gacctgattc ggtgattgcg ctgccagtga
     3961 taacagctcg tttcgtgcct gggttagttt ttcgtgcccc aggttaccgt tgtccagcag
     4021 ttccatatca aaacctgacg cggtacccag ttcagccacc gcgggtaagt tgaacgggaa
     4081 gacgacggct ttattgatac tgcttagcgc aatcattgcc cgctgaatga tcgcggtaac
     4141 cgagttttcc tcaccgacac gttcagacca cggcttgaga ctgataaacg ccaggccgtt
     4201 gttttgcccc tggccgctga agccaaagcc gccaacggta aacaccgact ggacattatc
     4261 tttctcttta gtcagataat aatccgtcac ctgttgcagc actttcgtgg tgttaaccat
     4321 ggtggcaccg gaaggtaact gcgcggtggt cataaatacc ccctgatcct cttctggtaa
     4381 gaaagaggtc ggcgtgcgca ggaacagcac cgccatcccg gcgcaaatca ggaggtagac
     4441 caccatgtag cgaccggtac aacgcaacag tgagcgggtg ctatcggtat agtgctgagt
     4501 tgatttttca aacagcgtgt tgaagcgtgc gaacagggcg ttaggtttgt gaccgccttc
     4561 cggcgcggct ttcagaatgg tagcgcacag ggcaggggtc aggctcattg ccacaaatac
     4621 tgaaagcagc atggaggaga tcagcgtgat ggaaaactgg cggtagatct ccccggttgc
     4681 accgctcata aaggccatcg gcataaacac tgcggaaaga acaacggcaa taccgaccag
     4741 cgcacgttgg atctgcccca tcgatttatg cgtcgcttcc ttcggcggta gcttatcttc
     4801 cgcaatgaca cgctcgacgt tctccaccac cacgatggcg tcatccacca gtaaccctat
     4861 cgccagcacc atcccgaaca tagtcaaagt gttgatggtg aaaccgaccg ccgacaagat
     4921 cgcaaacgtc ccgagaataa ccaccggtac ggcaatcgtc gggatgattg tggcacggaa
     4981 attctgcaaa aacagataca tgaccaggaa gacgaggatg atagcctcaa ccagtgtttt
     5041 gaaaacttcc tgaatagaaa tttcgataaa cggcgtggtg tcgtaaggat aaaccgtctt
     5101 cagacttgcc gggaaatagg ctgataagcg gttcagttcc tctttgactg cccgcgaggt
     5161 atccagggcg tttgctccgg tagccagttt gatggcgatc ccggcagcag gtttgccgtt
     5221 atagcgcgcc acggtggaat aatcgtccgc cccaagttcg acgcgggcga catcacgcag
     5281 cagcacttgc gaaccatctt gctgaacttt caacaggatt ttgccaaatt cttccggcgt
     5341 ttgcagacgc gtctgcacaa tgatcgaggc gtttagttgc tggtctgccg cctgtggcat
     5401 gccacccagt tgaccaccag aaatctggtt gttttgcacc ttaatctggg aaataacatc
     5461 ggaaggtacc agattgtatt tattgagttt ttgcgggtcc agccagatac gcatggcgta
     5521 ctcggaacca aagagctgta cgctaccaac gcccgcggta cggcttagcg ggtctttgat
     5581 attagacgct acatagtccg cgatatcgta ctggtttagg ctgccgttat cagaaataaa
     5641 cgccgctacc atcaggatat tactgctcga tttatcgacg ctaatcccct gctgctgcac
     5701 tgcttcaggt aatgaaggca tagcgagttg cagtttattt tgcacctgaa cctgtgcgat
     5761 atcaggagat gtcccagtct cgaaggtcag agtgatagag gcattacccg ccgcatcact
     5821 ggttgaagac atgtacatca ggccatcaag cccattcata ttttgctcaa tcacctgagt
     5881 gaccgagtct tctaccgttt gcgcatcggc acccggatag gtagcgctga cggtaatggt
     5941 cggtggcgca atctgcggat actgcgcaac cggtaagttc atgatcgcca gaccacctgc
     6001 aagcatcata ataatggcaa gtacccaggc aaaaaccggg cgatcaataa aatagttagc
     6061 catgaaagtc cccttaagcc tgcaacgtta ttgtttcgat tcggtgctgg cgttttcctg
     6121 gctggaggaa attgctcgtg ctttgatacc cggacgaatg cgttgcaaac cggaaacgat
     6181 cacccgatcg ccagcctgca agccagaggt gacaacccac tggtcgccga tggctttgct
     6241 ggcttcaatt tcgcgtagct gcacgacatc gtctttatcc agaatgagcg ccgttgcttt
     6301 accctgggcg ttgtgggtga cgccttcctg cggcaccagt aatacattct ggcggctacc
     6361 ttcatccact aatgccgtga catacatgcc aggcagcaag tcaccatttg ggttggggaa
     6421 aatcgcccgt aacgtcacgg agcccgtggt ttcatcaacc gtcgggtcgg agaatttcag
     6481 cgtgccggtc tggctgtaac gtttaccatt ttccagattg agctgtactg gcgtactgcc
     6541 ctgaacctgt ttgatttgcc cactggcgac ctcttctttc atgcgtaaga aatcctgcac
     6601 cgactgcgtg agatcgacat aaatcgggtc cagacgttgt acggtaacca gcgaatctgc
     6661 ctgattagcg gtaacgagtg cgccgacggt caccgacgat ttcccgctga cgcccgtaat
     6721 cggcgaggtg acattcgcgt attgcagatt gatcgtcgcc tgttcaacag ccgctttggc
     6781 gacggtgaca ttggcttctg cttcattcaa ctgggtgcgc gcggtgtcgt aatcctgacg
     6841 gctaacgtag ttggtcttca gcaacgatgc ctggcggtta aaggtgatgc gggcattgct
     6901 ggcggtagag agcgctttcg ccagcgagcc tttggcggag tttagctcgg cctgtaaagg
     6961 tgcaggatca atctgataca gcgaatcgcc ctggttcact ttatcgcctt cgataaagtt
     7021 gcgtttaatg ataataccgc ccacctgggg acgtatctcg gcaacttcat aaggaacggt
     7081 tctaccgggc aattcgctca acacattgac cgacccaggg gagagtgtga cgacaccgac
     7141 ctcaggcgtc atggcggcgg cgttttccgc cgatttgtca tcgcaggcgg tgagcatcgc
     7201 gccgcagaat aacaacggta ttaacagctt tcttcttctg ttcattttag tccctgaaaa
     7261 ttcttgagaa ataacggttc tttaaattgc gcgcgaggag agatatgagg gatttacaga
     7321 ccagcggcat agtggggttg tcagccagta aggtgggata caggcatagt gatcgacatg
     7381 gtgaggtcaa cgacaaaaaa aatgttgccg ttctgccaac agttcctgcc agcattcggg
     7441 caacgaaggg ttctactggt ggatacacat accaggggaa taaagtcgtt ttagcctctc
     7501 ctttagtaat caccgggggt aatgaaatca gcatctgcat ccctcgtcat gccagccatc
     7561 aatttcagtt gcttatgtcc tgactaaaaa taagatgtga tacccagggt gacgatgtcg
     7621 ctcatacgtc ttagtttcag cttgtacata atgttgaatt tctgaatctt taatgtttgt
     7681 tgcgaaatat taagcgccga cgtgatattg ctttcatttt tatttttcag aatcagttgc
     7741 ataattttga actgattatg tgaaaaacat aattgtgaat tcttatgggg caagtgttta
     7801 ccataagtaa tctcaagaag atttttgata ctttctttgc ggctaacaaa ataaataggc
     7861 gctttagctt ttagtttact gatgtgataa ggtgccaaaa caataattct ctcggcatct
     7921 aatttctcca gaaatttaat cgcttcttca tcaaggatat gattgtgata ggtatcaatg
     7981 ataaccttga agggtttatt gcctactttt ttgatctctg acagggaatt gatttttatc
     8041 atttcgtgat tatctttcaa ctgccaaaag ccctgtaaaa gaaaagaatc tttcgtcatg
     8101 agaaaaatca taacttgctc cttagccgtt atcgtttcga acatgtcatc cttttcatta
     8161 tttacatcct tgtccgaatc gttgttcaat atagtaaacg cctataagaa ctttgccctt
     8221 cgtgaactaa tacgtctatt aacactaatc aatacttcag atttatccat cacgcactcc
     8281 atatattgat cctaagtaag attcctggtt gttatcagct tgtaaatagc aaacaaaaag
     8341 ttaacgtttg cggattactg cccaagaata agttatcaat aaccagtttg tgatctctga
     8401 agaatattac taaagttaaa atgtaatccg atttaaatat cgagtctcct tgtttcgact
     8461 taagctggca attggattgc cagctttctt ttaacgcatt gaaattacct gagtaaataa
     8521 cacattcatg tcttcaggtt tttatatagc gtctggagac acggatattt atgcaatgta
     8581 atcaacgcca gttgcaaaaa aatgaacccg gattacttat ttaaccgccc atagcatcaa
     8641 cgcttacgga actctttgtt cactttccca cagcactttt aaaacattaa acctgactac
     8701 ggaaaatatc agccatgata atgtttgaaa tggctaattt gccatggagt gaaaaaaatt
     8761 agatgaaatt cagtaggttg aaataatcac tagcaggtaa ttatttcaat gatagtgcgc
     8821 aattgatcta caactccgcg tagcggagag agtattaatc ggatcatagt cacatcaagt
     8881 gactatgatc cgggtgacaa ccgggataat tattgctgct taacgaacaa actggcgaag
     8941 ctgaacaggc tggcggcgct aaatatcagt tcaattccca ccagtgtgga aaccagcgtt
     9001 acagacacca tcggcgttgc accaaggaat atccaggcaa tgacgatatc cagcacacca
     9061 ataacgagct gtagccagct gcctttcatt gaacgctgac gataccaact catcaggcga
     9121 ataacccctg caacacagaa caaaccggca ataaatgccg caatggcaaa aatgcccagc
     9181 tccggtgcgc ggatgaagaa atagccgatc aataaatagg cgactgcgac gaggaaaccg
     9241 gataataccg gccagaaatt atgactgcgg ttgctgaata acccgacaat aagcgcaata
     9301 cccgagcaga ttaataatgc acccactact gtgcttaaaa tatcgccaga gacgaacggg
     9361 aaactgatac acagcaaccc gacgataaac agcagcacgg caataaactg gattgctctg
     9421 cgatgttttt taagcatctc cagatcaaac ttcaaaattg ttgccttatc tatatataac
     9481 atagaaccac cctataaaat taagaagaaa atcccctgct atcaatctat gccaaaaacg
     9541 cgtctaagaa tgcagtcgat ttaataaaaa tttcctaatt gcagtatctg atgcatctgt
     9601 aactcattgt attgaaataa aaatatctga ttttgatatt ttccatcaac atgacatata
     9661 cagaaaacca ggttataacc tcagtgtcga aattgattcg tgacggctct ttcactttat
     9721 agttgaggat attacgatga aaaaagtatt aggcgttatt cttggtggtc tgcttcttct
     9781 gccagttgtg agcaatgcag cggatgcgca aaaagcagct gataacaaaa aaccggtcaa
     9841 ctcctggacc tgtgaagatt tcctggctgt ggacgaatcc ttccagccaa ctgcagttgg
     9901 ttttgctgaa gcgctgaaca acaaagataa accagaagat gcggttttag atgttcaggg
     9961 tattgcaacc gtaaccccag ctatcgttca ggcttgtact caggataaac aagccaactt
    10021 taaagataaa gttaaaggcg aatgggacaa aattaagaaa gatatgtaat tccgggaatg
    10081 cgttacatcg tacttccttg catattgaac aggccggaat atcttcttta aaagcagcta
    10141 ttcctcctgt tcatatataa tctctatatt gaatgggtta caaaatgaat atttcatctc
    10201 tccgtaaagc gtttattttt atgggcgctg tagcagcttt gtcactggtg aacgcacaat
    10261 ctgcgctggc agccaatgaa tccgctaaag atatgacctg ccaggaattt attgatctga
    10321 atccaaaagc aatgaccccg gttgcatggt ggatgctgca tgaagaaaca gtatataaag
    10381 gtggcgatac cgttacttta aatgaaaccg atctcactca aattcctaaa gtgatcgaat
    10441 actgtaagaa aaacccgcag aaaaatttgt ataccttcaa aaatcaagca tctaatgact
    10501 tgccgaatta atgaggtaca agtaaaaagg agtagcaagt tgagccatct tgctgctcct
    10561 ttttgcattt ttatatgaca gcagaattta ttatacgtct tatactcgcg gcaattgcct
    10621 gtggcgctat tggcatggaa aggcaaatac gcggcaaagg agcagggtta cgcacacatg
    10681 tattaattgg catgggaagc gccctgttta tgattgtttc gaaatatggt tttgctgacg
    10741 tgctgtcttt agatcacgtc ggactggacc ccagccgtat tgctgctcag gtggtgacgg
    10801 gcgtcgggtt tatcggtgca ggtaacattc tcgttcgtaa ccaaaatatt gtaggtctga
    10861 cgactgcagc ggatatctgg gtgaccgccg ccattggtat ggttattggc agtggtatgt
    10921 acgaactggg tatttatggt tcagtgatga ccttgctggt gctggaagtc ttccatcaat
    10981 taaccttccg cctgatgaac aaaaattacc atttacagct gacactagtg aacggtaata
    11041 cggtatcaat gctcgactgg tttaagcagc aaaaaataaa aactgattta gtttcgttgc
    11101 aggaaaatga agatcatgaa gtggtcgcca ttgatatcca gcttcatgcc acaacgtcga
    11161 tagaagatct gttgcgatta ttaaaaggta tggcaggggt gaaaggcgtt tcaattagct
    11221 aatctgtata gttatttatt cgcaggagta ccagtgcggt actcctgttg cttttaatga
    11281 tcgaggacga tcagtggtga atggttggca ggatctttaa gtacggtaat atccgcgccg
    11341 tagagcattg tcagcgcctg ctgtgtcaat acgtcctgtg gtttaccatt tgcgataacc
    11401 tttccttttt gcattaatac aacacggtct gcataacgcg cggcaagatt tagatcgtgc
    11461 aatatgcagc agacattgaa ctgccgctcg tgaactaact ggcgtaataa acgaaacaag
    11521 tgctgctgat ggtgaatatc cagcgccgaa gttggttcat caagaaataa ccatttcggt
    11581 gagggagtag gttcccagag ttgcaccagc aggcgcgcta gttgcacgcg ctgttgttca
    11641 ccaccggata attgccgata gtcgcggttt gctaacgcct gacagtcaca taacgccatt
    11701 atttgtgctg tttcattatc ctgattacca gtacgatgcg gatgccgccc catctggata
    11761 acttcttgta cgctgaatgg aaacgccatg tggctatttt gacgcatcac ggcccggtgt
    11821 ttcgccagtt cagtaatcga ccattcattt aatggcttat taaacaaccg acattcgccg
    11881 ctatcaggtt gaagatagcc ggttaactgg cgaagcagcg tagattttcc cgcaccatta
    11941 gggccgagaa tggcaacaat ttccccaccg ggaaaagtca gcgagacatt atcagtcaaa
    12001 cgccgcccct gaaggctata aaccagattc tgggcgctga tcatgtgatc ctccgatttc
    12061 gcagaatcaa ccacataaaa taagggccac caagcaggct ggttaataat cccacgggca
    12121 tttctgctgg ctgtaccagc gtgcgggcaa gcgtgtctgc catcagcaat aaacaggctc
    12181 cggcgagggc ggaacaaggg attagccagc ggtgatttgc tccggtagtc atgcgaatca
    12241 gatgcgggat caccaggcca ataaagccga taatgccgct taccgatacg gcagcaccaa
    12301 cgagcagtga gctcactaac agtaattgct ggcgtttacg cttaacgttc acgccgaggt
    12361 aatgggcttc ttcatcaccg agctgcagta aattcagcgt tccggcgaga caagttgttg
    12421 cgataatggc cggaaggatg aatgaactgg caaccaataa cgtcggccat tgcgcctgtc
    12481 caagattgcc catcatccac aatgtgagtt gtcgtagctg ctgctcatcg ccgatatagc
    12541 tcaggatgcc gatcgctgcg ccgcacaggg cattgatggc gataccggcg agtaataatt
    12601 gcatcatgct gccatcgcga tggcgctgcg tgatgagaaa aatgatggtg cagactacta
    12661 aacttccggc aatggcaaac accatttgct catagagcac cagaaccact gggaaggaga
    12721 agggcagcac aatagcaacg ccaaccatta atgcagaacc gctactgacg ccaagtaatc
    12781 ctgggtcagc cattgggttg cgaaaaagcc cctgcataat tacccctgcg gttgctaacg
    12841 ctgcacctac cagcaccgcc agcaaaaccc gtggtagacg aatattgagc cagatatcgc
    12901 ggtattcctc atcaaaaagg gcatcaaaac tgatctttaa cgctccctga ctggcaccga
    12961 acagcaccag ggcaagcaat aatagtgata gccccataaa gaccctggcg gatgaaaatg
    13021 aatccttgag cacgcaaaac tccgttacga cattacggcg cggacatgcc gcgccggggt
    13081 atcacgccgg tttcaggtcc ggttcaatca gactgtagac ctgctggtta agtgctggcg
    13141 catttgccag ttcatggata tgtgcggcga catcggcacg gcggacaaaa ccatgacact
    13201 cctgattttg tacccgttgt gctttgcctg ttgctgcgcc gtcaagcaga ccaccagggc
    13261 ggagaatggc gtagtctagc tggctggttt gcagccagct ttccgccagc gttttttccc
    13321 gtaccgcctg accaaacgca gcctttgccc gctccgataa aaacggccag ctatcgccgc
    13381 atcccaaaga ggtcaccaga atcatgcgac tgatgccggc cttttcggcc tcatcaatca
    13441 ctgttctgtg cgccagataa tcctgagctc ctcccattgt tgagatgata agcgcatccg
    13501 ggccagctgc gcggcaagca gccgcgacta cgctggcatc acaggcgtcg cctgtaaaaa
    13561 cctgtacgcc ttgttgcgcc agtttcgtgg cggcatcggc atgacgaatg acagcaacga
    13621 ccggacgttg ttccgccagc gccagttcaa gcgttctggc acccacacct tttccgcctg
    13681 cgccaaagag tagccacggc gtcatcaggc gtgttccttt aacgatgccg ccagagtatg
    13741 gaaggcgctg acttgttcgc tcaggagctg acgatggtca tccctgccga ggaagatttt
    13801 cagcatggcg ctgccttctt tgttgaaaaa gagaatggac gcggtgtcca tgcccataaa
    13861 tttacgttcg attaaggcaa tatgcgtgca gttttctgct ttgatatgcc cggacatgcc
    13921 gtgctttccg cgtaggttga aatagccgtg gcgatggaaa cccgaaggca gttcgccgct
    13981 aaattcgagg attacatcgg cagtatgtac taacgtggtg acgttgcccc attcgcagac
    14041 ggtatcccag acagtatcga atttatcgcc tggcactacg gtagagaagg gtagatttct
    14101 cactacctcc agcagtgtgg tgttgtattg ttcggctacc acctccagcg taccgtccgg
    14161 ctccgttttc aaaaattctt gtaacgagac atggctcata aatcactcct gttatggttt
    14221 ttcacgcaca atcgctggcg cattaagtac ctgaattagt tcattaagag actgcaaaat
    14281 attgcttgcc cagaaacgac cttcattagt cagccgcagg caagttgaag catcgcgact
    14341 taagcctttt tggtgccatt gagccagtaa cggcgcgagt ttttcggcat gtggcgtaag
    14401 ttcatccagc ggtacacgcg ctgtttcaac acctgactgc aaggtatgac gccattgcgc
    14461 attacgttcg gcgttacgca tgatcatcat cagcggtttt ttgcctgccg cgacggattc
    14521 atgccaggtc tgaagattgc gttcgttcat ccaggagtaa ccattaatcg aaccgccggc
    14581 tccggaacca aaggccagac aatcggcacc ttgttttatc agcaggttat agagattgcg
    14641 ttcgcgtgtg gtacggcccc agtggctgtt actaatacag cgccaaccgg cttcgtccat
    14701 gaagtcacac ccttgcagat aaagatcgcg acgttctgcc ggagagggca cggtagtacg
    14761 cccattttcc acggctttgc ccagcggtgt attgggcagg acattgagcg catagagatc
    14821 gacgccatcg agaccgatat cgcgggcaat agccagatct tctccccagg tttgcgcatc
    14881 ctgacccggc aggccaaaaa gcaggtcaca gaccaccgca gcacggtcac gtttgaccag
    14941 gctttccata aacgcaatag cggttgggcc atccgaggtg cgggccattt tcttgcggat
    15001 tttgctgtta aacgactgga taccaattga gaagcggttt gctcctgcat caaggcaagc
    15061 atcgattcgc tcagcgtcaa aattcaatac ccgtccttcg atagtgattt cacaatcagg
    15121 ggccagtggc agcttttctc gtaatgtggt gatgattctg gccaaatcgt gtgccgaaag
    15181 tgcggaaggc gtaccgccac cgaaatagac cgcgtggatg ggggcagact gatgcaatac
    15241 gctgtccgct tccatttcta tttcacgaat cagggcgtcg gtataatgag cacacgcatc
    15301 ttcattaaaa cggttctgat aaaaaccaca aaacgtgcag tgtgtcgcac aaaaaggaat
    15361 gtgtaggtaa accagtcgct tacgaggtga tgccgtttga ttgatcatct cttgccaggt
    15421 ctgtgccagt tgctctttgg caactggaat ggccccacga aacggcatca tggcgcgcct
    15481 gtctttaaat ggctgatcac cgtctaaagc aaaatgtggc gtgagatcca gggtgttatt
    15541 tgcgttcatt cgtctgattt aaatccattt attgcggcca aagctgctgg tgtaaagact
    15601 caacgacatc agtaatacgc ggacccattc ccagaatcag gttttgatca acggtgataa
    15661 tgcgctggtt tttccatgcg gcagtgtggg taattccagc aatagaacgt agccgattaa
    15721 tatcgccatc gaccatttgt gaggttacga caatcacttc agggttagcc gcaatcagtg
    15781 attctgcact gtagcttttg tactgctggt ggggagcgac gttttctgct ccggcaagcg
    15841 aaagaatggc gtccgcgacg ctgcctttac cggcaacttg tggcgcactt ccgcctgcgg
    15901 agagaataaa catggcttta accggggctt ttttggccgc cacgttttgc tgtactcgct
    15961 ccaggcgttg gctgatttgt gtcaccagcg cctcgccttg ttcaggaacc tgtagcgttt
    16021 ttgccagttg gcgaatgttg gcgtacatct gctcaagggt ggctggcaca cgcggcaggg
    16081 tgacgacgtt gactttctgc gctcgtagtt ggtcgagcac aatttgtggc ccggcatcct
    16141 gccaggtaat tacgctatcc gggcgaagtg acaaaatgcc ttcgctgctg agttgtttcc
    16201 agtagccaat atgcggcagt ttggcggttt ctggtggata agatgtcgtt tcatcgacac
    16261 caaccacgcg ttcgccagcg cccatcgcgt agattagctc cgtcagcgat cctcctgcga
    16321 ccacgatacg ttcggcagcc gttacgctaa aagcccagct aagagccagt actgccgtaa
    16381 acaatttttt tcgttttact gcggtcttcg cggcagaggc cacggctgaa atacataacg
    16441 tcagtggaga aaaaagaaac ggcctggtga tgatccttgg cataatatga gaactcttat
    16501 caaattgaga atgatcatga ttatcatttg agttatatat gaaatacaac cgtcgattca
    16561 atacgcaagg cgttacaagc gtatttattt ttgattttta tcaagatgct tcgtcagcta
    16621 acgttcttgt taccgactaa atttcatcgc gggaaaaagc tgagaaccca gagatatcga
    16681 ggcttgcaga gcaaagagaa gaaaaaatta tcactgttaa cgacaaaatt tagtgatgag
    16741 gcatgggggg ataaccataa aaacaaggtg gtttacaggg cgcaggatag gtttcataac
    16801 cccctgttta ttgtgttaat ggtggttaaa aaaaacttga tcataaaaaa ttgatcgata
    16861 ataattctca ttatcacgtt tgcatttaac aaaacacgac catatggata acaaaaccat
    16921 agcatttgca agggataacg cacagctctc ttcactcctg atggggcata tcgttttgcc
    16981 tgacaaagag ggcgtgcttt tgcgtaattg ccgagttatc aggcaatttc atgggatata
    17041 aacgcctgta aactcacccg gcgaatatta tcgctctgaa ttttaaataa atttattttt
    17101 cgccagccac cccggtatta acccaggcag ccagcatttt ttattttcct catggagaat
    17161 cgctatgtca cgtccgcaat ttacctcgtt gcgtttgagt ttattggcct tggctgtttc
    17221 tgccaccttg ccaacgtttg cttttgctac tgaaaccatg accgttacgg caacggggaa
    17281 tgcccgtagt tccttcgaag cgcctatgat ggtcagcgtc atcgacactt ccgctcctga
    17341 aaatcaaacg gctacttcag ccaccgatct gctgcgtcat gttcctggaa ttactctgga
    17401 tggtaccgga cgaaccaacg gtcaggatgt aaatatgcgt ggctatgatc atcgcggcgt
    17461 gctggttctt atcgatggtg ttcgccaggg aacggatacc ggacacctga atggcacttt
    17521 tctcgatccg gcgctgatca agcgtgttga gattgttcgc ggaccttcag cattactgta
    17581 tggcagtggc gcgctgggtg gagtgatatc ctacgatacg gtcgatgcaa aagatttatt
    17641 gcaggaagga caaagcagtg gttttcgtgt ctttggtact ggcggcacgg gggaccatag
    17701 cctgggatta ggcgcgagcg cgtttgggcg aactgaaaat ctggatggta ttgtggcctg
    17761 gtccagtcgc gatcggggtg atttacgcca gagcaatggt gaaaccgcgc cgaatgacga
    17821 gtccattaat aacatgctgg cgaaagggac ctggcaaatt gattcagccc agtctctgag
    17881 cggtttagtg cgttactaca acaacgacgc gcgtgaacca aaaaatccgc agaccgttga
    17941 ggcttctgaa agcagcaacc cgatggttga tcgttcaaca attcaacgcg atgcgcagct
    18001 ttcttataaa ctcgccccgc agggcaacga ctggttaaat gcagatgcaa aaatttattg
    18061 gtcggaagtc cgtattaatg cgcaaaacac ggggagttcc ggcgagtatc gtgaacagat
    18121 aacaaaagga gccaggctgg agaaccgttc cactctcttt gccgacagtt tcgcttctca
    18181 cttactgaca tatggcggtg agtattatcg tcaggaacaa catccgggcg gcgcgacgac
    18241 gggcttcccg caagcaaaaa tcgattttag ctccggctgg ctacaggatg agatcacctt
    18301 acgcgatctg ccgattaccc tgcttggcgg aacccgctat gacagttatc gcggtagcag
    18361 tgacggttac aaagatgttg atgccgacaa atggtcatct cgtgcgggga tgactatcaa
    18421 tccgactaac tggctgatgt tatttggctc atatgcccag gcattccgcg ccccgacgat
    18481 gggcgaaatg tataacgatt ctaagcactt ctcgattggt cgcttctata ccaactattg
    18541 ggtgccaaac ccgaacttac gtccggaaac taacgaaact caggagtacg gttttgggct
    18601 gcgttttgat gacctgatgt tgtccaatga tgctctggaa tttaaagcca gctactttga
    18661 taccaaagcg aaggattaca tctccacgac cgtcgatttc gcggcggcga cgactatgtc
    18721 gtataacgtc ccgaacgcca aaatctgggg ctgggatgtg atgacgaaat ataccactga
    18781 tctgtttagc cttgatgtgg cctataaccg tacccgcggc aaagacaccg ataccggcga
    18841 atacatctcc agcattaacc cggataccgt taccagcact ctgaatattc cgatcgctca
    18901 cagtggcttc tctgttgggt gggttggtac gtttgccgat cgctcaacac atatcagcag
    18961 cagttacagc aaacaaccag gctatggcgt gaatgatttc tacgtcagtt atcaaggaca
    19021 acaggcgctc aaaggtatga ccactacttt ggtgttgggt aacgctttcg acaaagagta
    19081 ctggtcgccg caaggcatcc cacaggatgg tcgtaacgga aaaattttcg tgagttatca
    19141 atggtaatca tctgccccga tatttcgggg catttatctg gaaggaagag agacaatgaa
    19201 ccactacaca cgctggcttg agttaaaaga acaaaatccc ggaaagtacg cgcgtgacat
    19261 cgcaggttta atgaatatca gcgaagcaga actggcattt gcacgcgtca cgcacgatgc
    19321 gtggcggatg cacggcgata tccgtgaaat tctggcggcg ctcgaaagtg ttggcgaaac
    19381 caaatgtatt tgtcgtaatg aatatgcagt ccatgagcaa gttggtacgt tcacaaacca
    19441 gcatttgaac ggacatgccg gattgatcct caatccgcgc gcgctggatt tacgtctgtt
    19501 tctcaatcaa tgggccagtg ttttccacat caaagaaaac acggctcgtg gcgaacgcca
    19561 gagtattcag ttctttgatc atcagggcga tgcattacta aaagtttatg ccaccgacaa
    19621 taccgatatg gcggcatgga gtgagcttct ggcacggttt atcaccgatg agaatacgcc
    19681 gcttgagtta aaagccgttg atgcgccagt tgttcaaacg cgagccgatg ccactgtggt
    19741 cgagcaagag tggcgggcga tgaccgacgt tcatcagttt tttacgttgc tcaagcgcca
    19801 caacctgacg cgccaacagg cgttcaatct ggtggcagac gatttggcct gcaaagtatc
    19861 caacagtgcg ttggcgcaaa ttcttgaatc tgcacagcag gatggtaatg aaatcatggt
    19921 gtttgttggc aaccgtggct gcgtacagat tttcaccggt gtggtagaaa aagtggtgcc
    19981 aatgaaaggt tggctgaata ttttcaaccc gacgtttact cttcatctat tagaagagag
    20041 cattgctgaa gcctgggtta cccgtaaacc gaccagcgat ggctacgtaa ccagtctgga
    20101 attgtttgcc catgatggta cgcagatagc gcaactttat ggtcaacgta cagaaggcga
    20161 acaggagcaa gcgcaatggc gtaagcaaat tgcttcgctg ataccggaag gcgttgctgt
    20221 ataacgtata attaacggga ggtctggcta tttattgctg ggcctcttta tattgatcaa
    20281 tcgttcacac cagataatca atatgctgat ggcgtaccag ctcattgatc cgcttaagat
    20341 tgaggatcat catgatattg tgtttatggc agtagaacgt ttttagcgat attttcagtt
    20401 tctccagaat atcttccgtt gtcatttgat tcgcaatgca gctcagaacc tgatgttgct
    20461 tgcgcgaaag tgacacattc gcttcaggca cagagcagta actgccattg atgagtgcga
    20521 caaaatcaat gatagtgata gtccgtggaa caaaggtcac cggcactttc cccaggcagc
    20581 gtttaatatt aaagggggag aaaataatga catggacagg ttttaatcgt tcaatttgag
    20641 tgagaaaatt agaatgaaat acattattca ttaatgtatc aataatgacg aaggcattct
    20701 gatgcaacat tacgtcgatc tcttcttcgt tctgtatatg aacgacatta cctttactca
    20761 gaatgttttt catcgcggtg aagaacatcg tatccctggt aattataaga aacataaccg
    20821 actcctggtg cggacattaa ctttataatc agtttgccaa aggtaattaa caggacaggg
    20881 cttataataa taatagttag cttactgtat attaatttta tgtaatacat gatgcagatg
    20941 ctgagaaagc atctgcatct ttcggtggtg ttatttgacc agctcaggtg ttacctgact
    21001 caccgcattg gtgtagtaag gcgcacccca gcccggttcc ggccagaatg caccatagcc
    21061 gtaatcccac aggttatagg tggtattaac cacttctctc aaatgccaca cctggatgcc
    21121 ctgcatattc acttccagga agttatacgg gactttattg ataaagccag gttgttcacc
    21181 ctgaatggtg ccgaggatgg taacaaagtg attacgatag ttcactggat caaggaagcc
    21241 gctttgtctg gcgagcagtc ggccctgata gttggcttca atatcaggct tcgcatagct
    21301 atccaacggt aatacagcga tttctaacaa cgtatccgtt ttgccattga taacgttgat
    21361 aaccttccca ccaaagcgcg cttgttgacc aacatataac cccggctggt tatgaacagc
    21421 aacaaaactt ttttgaatat caggttggtt attgcctttg atattttgcg gaattgaact
    21481 acatgcggca agcaaaaatg aaaggctgag tatgagtgca ccttttgtca tgttcatgtt
    21541 actatcctta tcaacaaata ttaaaaccat aatagatatt tctttatagt tttcatctga
    21601 ttctgaggcg atatgcctga tagtaacgta aacctgtctg atgtttatat cccttctgtt
    21661 tagttcaatt ccgttatttt ggggcgtggc atgagta
//
LOCUS       DAZFKF010000081        21674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFKF010000081 DAZFKF010000000
VERSION     DAZFKF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.081"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            250..1764
                     /gene="caiT"
                     /locus_tag="ACHKA1_003630"
     CDS             250..1764
                     /gene="caiT"
                     /locus_tag="ACHKA1_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO5681227.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1795..2937
                     /gene="caiA"
                     /locus_tag="ACHKA1_003631"
     CDS             1795..2937
                     /gene="caiA"
                     /locus_tag="ACHKA1_003631"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO5681228.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3066..4283
                     /gene="caiB"
                     /locus_tag="ACHKA1_003632"
     CDS             3066..4283
                     /gene="caiB"
                     /locus_tag="ACHKA1_003632"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO5681229.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4357..5910
                     /gene="caiC"
                     /locus_tag="ACHKA1_003633"
     CDS             4357..5910
                     /gene="caiC"
                     /locus_tag="ACHKA1_003633"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO5681230.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPKFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            6019..6804
                     /gene="caiD"
                     /locus_tag="ACHKA1_003634"
     CDS             6019..6804
                     /gene="caiD"
                     /locus_tag="ACHKA1_003634"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO5681231.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVDSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6810..7400
                     /gene="caiE"
                     /locus_tag="ACHKA1_003635"
     CDS             6810..7400
                     /gene="caiE"
                     /locus_tag="ACHKA1_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO5681232.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGHDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRTVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVAPKR"
     gene            complement(7519..7914)
                     /gene="caiF"
                     /locus_tag="ACHKA1_003636"
     CDS             complement(7519..7914)
                     /gene="caiF"
                     /locus_tag="ACHKA1_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO5681233.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8175..11396)
                     /gene="carB"
                     /locus_tag="ACHKA1_003637"
     CDS             complement(8175..11396)
                     /gene="carB"
                     /locus_tag="ACHKA1_003637"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO5681234.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11414..12562)
                     /gene="carA"
                     /locus_tag="ACHKA1_003638"
     CDS             complement(11414..12562)
                     /gene="carA"
                     /locus_tag="ACHKA1_003638"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO5681235.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETAIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(13018..13839)
                     /gene="dapB"
                     /locus_tag="ACHKA1_003639"
     CDS             complement(13018..13839)
                     /gene="dapB"
                     /locus_tag="ACHKA1_003639"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO5681236.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGSIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLHNL"
     gene            complement(14006..14920)
                     /gene="rihC"
                     /locus_tag="ACHKA1_003640"
     CDS             complement(14006..14920)
                     /gene="rihC"
                     /locus_tag="ACHKA1_003640"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO5681237.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAVRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVEAQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALAP"
     gene            complement(14986..15936)
                     /gene="ispH"
                     /locus_tag="ACHKA1_003641"
                     /gene_synonym="lytB"
     CDS             complement(14986..15936)
                     /gene="ispH"
                     /locus_tag="ACHKA1_003641"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO5681238.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(15938..16387)
                     /gene="fkpB"
                     /locus_tag="ACHKA1_003642"
     CDS             complement(15938..16387)
                     /gene="fkpB"
                     /locus_tag="ACHKA1_003642"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5681239.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16475..16969)
                     /gene="lspA"
                     /locus_tag="ACHKA1_003643"
     CDS             complement(16475..16969)
                     /gene="lspA"
                     /locus_tag="ACHKA1_003643"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO5681240.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(16969..19785)
                     /gene="ileS"
                     /locus_tag="ACHKA1_003644"
     CDS             complement(16969..19785)
                     /gene="ileS"
                     /locus_tag="ACHKA1_003644"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO5681241.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFAHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(19828..20769)
                     /gene="ribF"
                     /locus_tag="ACHKA1_003645"
     CDS             complement(19828..20769)
                     /gene="ribF"
                     /locus_tag="ACHKA1_003645"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO5681242.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(20777..20995)
                     /gene="yaaY"
                     /locus_tag="ACHKA1_003646"
     CDS             complement(20777..20995)
                     /gene="yaaY"
                     /locus_tag="ACHKA1_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO5681243.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            21098..21361
                     /gene="rpsT"
                     /locus_tag="ACHKA1_003647"
     CDS             21098..21361
                     /gene="rpsT"
                     /locus_tag="ACHKA1_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO5681244.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         5177 a         5826 c         5571 g         5100 t
ORIGIN      
        1 tcagatgcta tttttttacc cacaaaagaa cataacaaac tgattaataa caaaaattaa
       61 cggaaagacg tcctctattc acccgaaaca aaaatgtgac actaatcaca gaatacagct
      121 tattgaatac ccattatgag ttagccatta acgcgtccac gaggttaata ataattatat
      181 taaatgttaa caaaaataaa acaaacggga acgcaaaata aatattcgtt ttcacagtgg
      241 aattaactca tgaagaatga aaagagaaaa acgggaatag aaccgaaggt tttctttcca
      301 ccgttaataa tcgtcggcat actttgttgg cttacagtca gagatctgga tgcggcgaat
      361 gtcgttatta atgctgtatt cagttacgtc accaatgtat ggggatgggc atttgaatgg
      421 tatatggtgg tgatgctttt cggttggttc tggctggtgt ttggcccgta tgccaaaaag
      481 cgtttaggta acgaaccgcc agaatttagc accgccagtt ggatctttat gatgttcgcc
      541 tcctgtacgt ctgctgccgt actgttctgg ggatcgattg agatctacta ctacatctcc
      601 accccgccgt ttggcttaga accgaactcg acaggagcga aagagttggg gctggcttac
      661 agcttgttcc actggggacc tctgccgtgg gccacttaca gcttcctttc agtcgccttc
      721 gcttacttct tctttgtccg caaaatggaa gtgattcgcc ccagctcgac actggtgccg
      781 ctggtaggtg aaaaacacgc caaagggttg ttcggtacca tcgtcgacaa cttctatctc
      841 gtcgccttga tcttcgcgat gggtaccagt ctgggccttg ccacgccgct ggtgaccgag
      901 tgtatgcaat ggttgtttgg cattccgcat accctgcaac tggacgctat catcattacc
      961 tgctggatta tcctcaacgc catttgcgtc gcctgcggtc tgcaaaaagg ggtacgtatc
     1021 gccagtgacg tgcgtagtta cctgagcttc ctgatgctgg gttgggtgtt cattgtcagc
     1081 ggtgccagct tcatcatgaa ctacttcacc gattcggtgg ggatgttgct gatgtatctg
     1141 ccgcgaatgt tgttctatac cgatcccatc gctaaaggcg gcttcccgca gggctggacc
     1201 gtgttctact gggcatggtg ggtgatttac gccatccaga tgagtatctt cctcgcccgc
     1261 atctcccgtg gtcgtactgt gcgtgaactg tgcttcggca tggtgatggg gctgacagca
     1321 tccacctgga tcctgtggac cgtgctcggt agtaacactc tgctgttgat ggataaaaac
     1381 atcatcaaca ttccaaatct gatcgaacag tacggtgtgg cgcgcgccat catcgaaacc
     1441 tgggccgctc tgccactcag caccgccacc atgtggggct tcttcatcct ctgctttatt
     1501 gccaccgtta cgctggttaa cgcctgctct tataccctgg cgatgtctac ttgccgcgaa
     1561 gtacgcgatg gtgaagaacc accgctgctg gtgcgtatcg gttggtcaat tctggttggc
     1621 attatcggta ttgttctgct ggcgctcggc ggcctgaaac cgattcaaac cgccattatc
     1681 gccggaggat gcccgctgtt cttcgtcaac attatggtga cgctctcctt tattaaagac
     1741 gcgaaacaga actggaaaga ttaattaacc cccaaaatat caagaggttg aaagatggat
     1801 tttaatttaa atgatgagca ggaactgttt gtcgccggta tccgcgaact gatggccagc
     1861 gaaaactggg aggcctattt tgccgagtgc gaccgtgaca gcgtctaccc ggaacgtttt
     1921 gtcaaagcac tggcggatat gggtatcgac agtctgctga tccctgaaga gcacggtggt
     1981 ctggacgcgg ggtttgttac tctcgccgcc gtgtggatgg agctgggacg tctgggggca
     2041 ccaacctatg tactgtacca gttgccgggc ggattcaaca ccttcctgcg cgaaggcaca
     2101 caagagcaga tcgacaagat tatggctttc cgcggcaccg gtaagcagat gtggaactca
     2161 gcgattaccg aaccgggcgc gggctccgac gtgggtagcc tgaaaacgac ttatacccgt
     2221 agaaatggta agatttatct taatggtagt aagtgtttta ttaccagcag cgcctacacc
     2281 ccgtacatcg tggtgatggc gcgcgacggg gcttctccgg acaaacctgt ctacaccgaa
     2341 tggtttgttg atatgagcaa accgggcatc aaagtgacca aacttgagaa gctcggtctg
     2401 cgtatggata gctgctgtga aatcaccttt gacgatgtgg aactggacga gaaagacatg
     2461 ttcggtcggg aaggtaacgg ctttaaccgc gtcaaagaag agttcgacca tgaacgtttc
     2521 ctggtagccc tgaccaacta cggtacggcg atgtgcgcct ttgaagatgc ggcgcgctac
     2581 gccaaccagc gcgtgcagtt tggcgaggct attggtcgtt tccagttgat tcaggaaaaa
     2641 ttcgcccaca tggcgatcaa attaaactcc atgaaaaaca tgctgtatga agcagcgtgg
     2701 aaagcagaca acggcaccat cacctctggc gatgcagcga tgtgcaaata cttctgcgcc
     2761 aatgcggcat ttgaagtggt ggatagcgca atgcaggtgc tgggcggtgt cgggattgcg
     2821 ggcaaccacc gcatcagccg cttctggcgt gacctgcgtg tagaccgcgt ttccggagga
     2881 tctgacgaaa tgcagatcct gacgctgggt cgtgcggtgc tgaagcaata ccgctaagtc
     2941 cgtgcgctgc ctgatgcgac gctgacgcgt cttatcaggc ctacgggagc acgaatgtag
     3001 gccggataag gcgtttacgc cgcatccggc agtcatgcgc tacataaatt tttcaggagc
     3061 taataatgga tcatctaccc atgccgaaat tcggaccgct ggccggattg cgcgttgtct
     3121 tctccggtat cgaaatcgcc ggaccgtttg ccggacaaat gttcgcagaa tggggcgcgg
     3181 aagttatttg gatcgagaac gtcgcctggg ccgacaccat tcgcgttcaa ccgaactacc
     3241 cgcagctctc ccgccgcaat ttgcacgcgc tgtcgttaaa tattttcaaa gatgaaggcc
     3301 gcgaagcgtt tctgaaatta atggaaacca ccgatatctt catcgaagcc agtaaaggtc
     3361 cggcctttgc ccgtcgtggc attaccgatg aagtactgtg gcagcacaac ccgaaactgg
     3421 ttatcgctca cctgtccggt tttggtcagt acggcaccga ggagtacacc aatcttccgg
     3481 cctataacac catcgcccag gccttcagtg gttacctgat tcagaacggt gatgttgacc
     3541 agccaatgcc tgccttcccg tataccgccg attacttttc tggcctgacc gccaccacgg
     3601 cggcgctggc agcactgcat aaagcgcgtg aaaccggtaa aggcgaaagt atcgacatcg
     3661 ccatgtatga agtgatgctg cgtatgggcc agtacttcat gatggattac ttcaacggcg
     3721 gcgaaatgtg cccgcgcatg agcaaaggta aagatcccta ctacgccggt tgcggtctgt
     3781 ataaatgcgc cgacggctac atcgtgatgg aactggtggg cataacccaa attgaagagt
     3841 gctttaaaga tattggcctc gcacatctac tcggtacacc ggaaatcccg gaaggcactc
     3901 agcttatcca ccgtatcgaa tgcccttacg gcccactggt agaagaaaaa ctcgatgcct
     3961 ggctggcggc acataccatc gcagaagtta aagaacgctt tgccgaactg aatatcgcct
     4021 gtgccaaagt actgaccgta ccggaactgg aaagcaatcc acagtatgtt gcccgtgaat
     4081 caatcactca gtggcaaacg atggatggtc gcacctgcaa agggccgaac atcatgccga
     4141 aattcaaaaa taaccccgga caaatctggc gcggaatgcc ctcacatggc atggacacgg
     4201 ctgccatttt gaaaaatatc ggctacagcg aaaacgacat tcaggagttg gtcagcaaag
     4261 gtctggccaa agttgaggac taaacgcgtg gcgtcagtcg gcttgcctga ctagcgcccg
     4321 cccaataaca aacgaacaaa aatggataga ggtgcaatgg atatcattgg cggacaacat
     4381 ctacgtcaaa tgtgggacga tctggcggac gtttacggtc ataaaacggc gctgatttgt
     4441 gaatccagcg gcggagtcgt taaccggtat agttatcttg agttaaatca ggagattaac
     4501 cgcacggcaa acctgtttta tacgctgggg attcgcaaag gcgacaaggt tgcactacat
     4561 ctcgacaact gcccgaaatt tatcttttgc tggttcgggc tggcaaaaat tggcgcgatt
     4621 atggtgccga ttaacgcccg cctgttacgc gaggaaagcg cgtggatcct gcaaaatagc
     4681 caggcgtgcc tgctggtgac aagtgcgcaa ttctatccta tgtatcaaca gattcagcag
     4741 gaagatgcca ctcaattgcg gcacatttgc ctgacagatg tggcacttcc cgctgatgat
     4801 ggcgtgagtt cgtttactca actgaaaaat caacaacctg ccaccttgtg ctatgcaccg
     4861 ccgctatcga ctgacgatac ggcggaaatt ctcttcacct ccggcaccac ctcccgaccg
     4921 aaaggtgtgg tgattaccca ttacaacctg cgcttcgctg gatattactc cgcctggcag
     4981 tgtgcactgc gtgacgatga cgtctacctg acggtaatgc ctgcgtttca tatcgattgc
     5041 cagtgtactg cggcgatggc ggcgttttct gccggggcca cctttgtgct ggtcgagaaa
     5101 tacagcgccc gcgccttctg gggacaggtg cagaagtacc gcgccaccat taccgaatgt
     5161 attccgatga tgattcgtac gttgatggta cagccgcctt cagcgaacga tcggcaacac
     5221 cgcctgcggg aagtgatgtt ttatctcaac ttgtcggagc aggaaaaaga tgcgttttgt
     5281 gaacgcttcg gcgttcgctt gctgacgtct tatgggatga cggaaaccat tgtgggcatt
     5341 atcggcgatc gccctggcga taaacgacgc tggccgtcga ttggtcgggc ggggttttgc
     5401 tacgaagcgg agatccgcga cgatcacaat cgcccgctcc cggcaggtga gatcggtgaa
     5461 atctgtatta aaggcgtacc agggaaaacc atcttcaaag agtattttct caacccgaaa
     5521 gccactgcaa aagtgctgga agctgatggc tggctgcata ccggcgatac cggataccgc
     5581 gacgaagaag gcttttttta tttcgtcgat cgccgctgca atatgatcaa acgcggtggc
     5641 gagaatgtct cctgcgtgga gctggaaaat atcatcgcca cccatccgaa aattcaggac
     5701 atcgtggttg tgggtattaa agattcgatt cgcgatgaag ccatcaaagc atttgtggtg
     5761 ctgaatgaag gtgaaacatt gagcgaagag gaattttttc gcttctgcga acaaaatatg
     5821 gcgaaattta aagtgccctc ttatctggag atcagaaaag atctgccacg taattgctcg
     5881 gggaaaataa ttagaaagaa tctgaaataa atgaaacggc agggaactac cctgccagct
     5941 aataatcacg ctctaaaaca atatgcattt tttgcaggga tgttgtcgtc cctgaaaaag
     6001 caaaaatgga gaaaaggaat gagtgaatca ttacatctga cccgcaatgg atcaattctg
     6061 gaaattaccc ttgatcgccc aaaagcgaat gctattgatg caaaaaccag ctttgaaatg
     6121 ggcgaagtat ttctaaattt ccgtgacgat ccgcaattac gtgtcgccat tattaccggt
     6181 gccggagaga aattcttttc cgcgggctgg gatttaaaag cggcagcaga aggcgaagca
     6241 ccggatgctg actttggccc gggtggtttt gcgggattaa ccgaaatttt caatctcgac
     6301 aaaccggtta tcgcagctgt gaacggctac gcctttggcg gcggtttcga actggcgctg
     6361 gcggcagatt ttattgtttg tgccgataac gccagcttcg ccctgccgga agccaaactg
     6421 ggcatcgttc ctgacagcgg cggcgtgctg cgtctgccga agatcctgcc gcctgccatc
     6481 gtcaatgaaa tggtgatgac cggcagacga atgggcgcag aagaggcgct gcgttgggga
     6541 gtagtcaacc gcgtggttag ccaggcggaa ctgatggata acgcccgcga actggcgcag
     6601 caactggttg acagcgcccc gctggcgatc gcagcgctga aagagatcta ccgcaccacc
     6661 agcgaaatgc cggtagaaga agcgtatcgc tatattcgca gcggcgtgtt gaaacactat
     6721 ccatcagttc tgcattcaga agatgccatt gaagggccgc tggcgtttgc cgagaagcgc
     6781 gatccggtgt ggaaaggacg ttaacaaccg tgagctatta cgcctttgag gggttaattc
     6841 cggtggttca cccgacggcg tttgtccatc ccagtgccgt cttgattggc gatgtgattg
     6901 tgggagccgg tgtctacatc ggcccactcg cctcactgcg tggtgactac gggcggttga
     6961 tcgtgcaagc gggagccaat attcaggatg gctgcattat gcatggctac tgcgacactg
     7021 acactatcgt tggggaaaac ggccatatcg ggcacggagc gatcctgcat ggttgtgtga
     7081 ttggtcacga tgcattggtc gggatgaaca gcgtgattat ggatggtgcg gtcattggcg
     7141 aagagagcat tgttgccgcc atgagctttg tcaaagcggg ctttcgcggc gagaaacgtc
     7201 agttgttgat gggtacgcca gcccgcgcgg tacgcactgt tagtgacgac gagttacact
     7261 ggaaacggtt gaataccaaa gagtatcagg atcttgtcgg gcgctgtcat gcatcgttac
     7321 atgaaacaca gccactgagg caaatggagg aaaatcgccc ccgtttgcag gggacgacgg
     7381 atgtggcgcc gaaacggtaa tcttgccgga tgcggcgtaa acgccttatc caactctgct
     7441 ggagagcaac cgtaggccgg ataagatgcg ccagcatcgc atccggcgac caggtcgcac
     7501 ggtatcatcg aaatgacatt aacgacgcat actctttgac aacatcatct gccacttctg
     7561 ctcgcggtta agttccgtga gcggaacggc aggaatacgc ggtgtctttc ttacgccgac
     7621 taatttttcc cgactgttat tgcgcaggcg cgcgtaaatt tgctcatcga tatcgaccac
     7681 tttaaccagt cgctgacact ggcatccccg cccttccagc ttattaggga tcatcttaac
     7741 ttcgcagctt atttctgtga cttccgacag aatataagtc agggtattaa ccgccttgct
     7801 gtgttcaata tcgaaatgaa tagagatctc tcttgctatc acccaccgat tttcagccat
     7861 catccattcg gcgattaaca agtagagtgg tttttcaaca tatccttcac acatacgaac
     7921 tccagtttca caaattaaaa gtctggctat gactcgtttt gaagcaatta aacaaggcca
     7981 tattaaaatt gtggatatat cgatagtatg caacttcacc gtcgataagt gtgagctaaa
     8041 tcctgcttat gtcatcaaaa atatcgattc ccgaaaagat ttacttaatg ggcaaaaaaa
     8101 acggtcctca tcagagaacc gtattagtta ttcgatcaaa ttagcggatg aaaaatatcc
     8161 gccatgacac gctattattt gatctgcgcg tgcatttcct gcaccgaaat tactttttcg
     8221 gtcgcatcgg cattcagcgc catcgcggtg gcaaagccgc cgttcagggt ggtgtcgtaa
     8281 tgcactttat attgcagcgc actgcgacgg atcacgcggg agtcttcaat cgcgcgacga
     8341 cctgaagtgg tgttgatgat gtaggtatat tcgccattct tgatgcggtc ctgaatgtgc
     8401 ggacgacctt catgcacctt gtttaccaga cgcggattga tacccgcttc gcccagcaca
     8461 atcgccgtgc cgtgggtcgc atccagctcg aagccctgtt tcagcagttt tgccgccagg
     8521 tccaccacgc gttctttatc gccttcgcgc acggaaagca gcgcacgacc gtgtttcttc
     8581 atggtggagt tgctgcccag ctgcgctttg gcaaacgctt cagcgaaggt gcggcccacg
     8641 cccatgactt ccccggtaga gcgcatttct ggccctaaca gcgggtcaac gcccgggaat
     8701 ttattgaacg gcagcaccac ttctttcacc gagtagtacg gcgggataac ttctttggtt
     8761 acgccctgct cagccagcga tttgccagcc atcacacgcg ccgccacttt tgccagcggt
     8821 acgccggtgg ctttggagac gaacggaacg gtacgcgccg cacgcgggtt aacttcaatc
     8881 aggtagactt cgttgttttt caccgcaaac tgcacgttca tcaggccgcg cacctgcaat
     8941 tcgaaggcca gtttctgcac ctgctggcgc atcacatcct gaatttcctg acttaaggtg
     9001 tacgctggca gcgaacatgc ggagtcaccg gagtgcacgc ccgcctgctc gatgtgctcc
     9061 atgatgccgc caatcagcac catttcgccg tcgcagatgg catccacgtc aacttctact
     9121 gcgtcatcaa ggaaatgatc cagcaacact ggcgcatcgt tagacacgct aaccgccgtc
     9181 tggaagtagc gacgcaggtc agcttcgtca tagacgattt ccatcgcccg accgccgaga
     9241 acgtaagacg gacgcaccac cagcgggtag ccaatctctt ttgccttctc aactgccatt
     9301 tcaatagcag taacggtggc gttcgccggt tgtttcagtt tcagacggtc aaccgcatgc
     9361 tggaagcgtt cacggtcttc cgcacggtcg atagcatccg ggctggtgcc gataaccggt
     9421 acgccagcgg cttccagtgc acgcgccagt ttcagcgggg tctgaccgcc gtactgaacg
     9481 ataacgcctt tcggcttctc aatacgcacg atttccagca catcttccag agttaccggc
     9541 tcgaagtaga ggcgatcgga agtgtcgtag tcggtagaga cggtttccgg gttacagtta
     9601 accataatgg tttcgtaacc gtcttcacgc agcgccagcg aggcgtgtac gcagcagtag
     9661 tcgaattcga taccctgacc gatacggttt ggaccgccgc caagcaccat gattttttca
     9721 cggtcggtag acggattcgc ttcgcactct tcttcataag tggagtacat gtaagcggtg
     9781 tcggtggcaa actctgccgc acaggtatcc acgcgcttgt agaccgggtg caggtcatac
     9841 tggtcacgca gcttacggat ttccgcttcg cgtacgcccg ccagttttgc caagcgcgca
     9901 tcggcaaagc ctttgcgttt cagctggcgc aggaattcag cgttcaggcc agtgatgccc
     9961 acttccgcca ctttctcttc cagacgcacc agttcttcaa tctgtaccag gaaccagcgg
    10021 tcaatgttag tcaggttgaa gacgccgtcc acagacaggc ccgcgcggaa agcatcggcg
    10081 atgtaccaga tacgctctgc gcctgcgtct ttcagttcgc gacggatttt ggttaacgct
    10141 tccgggtcat ccaggctcac tttcgggtcg aatccagtcg caccgacttc caggccgcgc
    10201 agcgcttttt gcagggattc ctgctgcgtg cgaccaatcg ccatcacttc gccaaccgat
    10261 ttcatctgag tggtcagacg gtcgttagca ccggcgaatt tttcgaagtt gaagcgagga
    10321 attttagtaa ccacatagtc gatggacggc tcgaaggagg ccggagtacg tccgccagtg
    10381 atgtcgttca tcagttcgtc gagggtgtaa cccaccgcca gtttcgccgc cactttagca
    10441 atcgggaaac cggtcgcttt cgacgccagc gccgaagaac gcgacacgcg cgggttcatt
    10501 tcgataacaa tcaggcgacc gtttttcggg ttcaccgcaa actggacgtt ggaaccaccg
    10561 gtttcaacgc cgatttcacg cagcaccgcc atcgaggcgt tacgcatgat ttgatattct
    10621 ttgtcggtca gcgtttgggc tggcgcgaca gtgatggaat caccagtgtg gatgcccatc
    10681 gcatcgaagt tttcgataga gcagacgatg atgcagttgt cgtttttatc acgcaccact
    10741 tccatctcgt actctttcca gccgatcagc gactcatcaa tcagcaactc tttggtcgga
    10801 gagagatcca gaccgcgggc gcaaatttct tcaaactctt cgcggttata agcgataccg
    10861 ccgccgctac cgcccatggt aaaggatggg cgaataatgc acgggaagcc cacgtcagcg
    10921 gcaaccgcca gcgcttcttc catcgtgtgt gcgataccgg aacgcgcggt ttccagacca
    10981 attttcttca tcgctacgtc gaaacgacgg cggtcttctg ctttatcaat cgcatcggca
    11041 gtggcaccaa tcatggtgac gccgaactct tccaacacgc cctgacgttc cagctccagc
    11101 gcgcagttca gcgccgtctg accgcccatg gttggcagca ccgcgtccgg gcgctctttt
    11161 tcaataatct tgcgtaccac ttcccagtga atcggctcga tgtaggtcgc atcggccatt
    11221 tccgggtcgg tcatgatggt cgccgggttg gagttcacca gaatgacgcg gtaaccctct
    11281 tcgcgcaggg ctttacatgc ttgcgcgcca gagtagtcaa actcacacgc ctgaccgata
    11341 acaatcgggc ccgcacccag aatcaggata ctttttatat ctgtacgttt tggcatggct
    11401 cttttactcc tgattactta gcggttttac ggtactgctc aattaactcg ataaagtggt
    11461 cgaacaacgg cgcggcgtcg tgtggaccag ggctggcttc agggtgaccc tggaagctga
    11521 acgccggttt atcggtgcga tgaatgccct gtaacgtacc gtcgaacagg gatttatgcg
    11581 tgacacgcag gtttgcaggt aatgttgctt catccaccgc aaaaccgtgg ttctgggcgg
    11641 tgatcatcac cacgtttttc tcaacatctt taaccggatg gttgccgccg tggtgaccaa
    11701 atttcatttt gacagtcttc gcaccgctcg ccagcgccag cagctgatga ccgaggcaga
    11761 tgccgaatac cggaatagcg gtttcgagga atttctggat ggcggtaatg gcgtaatcgc
    11821 acggggccgg gtcgccagga ccgttggaga ggaagatgcc gtctggattc attttcagca
    11881 catcttccgc agaagtttgc gccggaacga tggtcaggcg acagcctcta tccaccagca
    11941 tccgcaggat gttgcgcttg gcaccaaaat cataagccac gacgtggaac ggcagctcgt
    12001 cttctttttt cgcttctggc aggccgccgg tcaacgtcca gctcccttgt gtccagctat
    12061 aggcttctgc ggtggtcact tcttttgcca gatccatgcc gttcagacct gggaacgcgc
    12121 gggctttttc taacgccagc gccgcatccg ggttatcgcc tgcgataatg cagccattct
    12181 gtgcgccttt ctcgcgcagt aaacgtgtca gcttacgggt atcgatatcg gcaatcgcca
    12241 cgatgttatg gcgcttcagg taagaagata ggtcttcggt attacggaag ttgctggcaa
    12301 tcagcggcag atcgcgaatc accagacctt gtgcatgtac ctgagaagat tcttcatcgg
    12361 cgtcattagt gccgacattg ccaatatggg gataagtaag agtaacgatt tgacgagaat
    12421 aggaaggatc agtgaggatt tcttgataac cggtcattga agtattgaaa acgacttccc
    12481 caaccgccga acctgttgcc cctatggccc gaccgtgaaa ctgggttccg tcttccagaa
    12541 ccaatagcgc tgacttaatc aaaacaccct ccagagaata ttcactcact ttatttgcat
    12601 attaattcac aatgatgacg taaatcaatg caaatctgct taccgacgaa tttctggcaa
    12661 acggcggcat tctggagata tgggcgctaa aagtcaactt aatggcggga ttttttatat
    12721 tattttgtgg ctcatgcatg ttttacaggc attaagcgac aaaaagatca tctaacctgt
    12781 tctggaaaac gcttgcgcag caagaagatt gcatgaagta aaaacgagta gaaaaaagtg
    12841 gaccaaatgg tcaaaattta cattaaaaca acaaaaatag aagacaaaac atgtgattta
    12901 tagcgtttgc ataaaattta aaagcataac accaaaaata aaagggctaa aaattaaagc
    12961 cctttatatt caataaatta aatgcgttac ttttgcatca ccataaacac tttgtggtta
    13021 caaattatgg agatcaagca catctcgcat atcaaaaaga ccactttcct taccactcaa
    13081 ccacaaagcc gatcttaccg caccgttagc aaatgtcatg cggctggacg ccttatgggt
    13141 gatctccaga cgctcgccaa tatcggcaaa catcgcggta tgttcgccaa cgatgtcacc
    13201 tgcacgcacg gtggcaaaac caatgctacc aggcacacgt tcaccggtat ggccttcacg
    13261 actgtagacc gcgcaatctt tcagatcttt atcaagggcg tgggcgatcg cctctcccat
    13321 tgccagtgcg gtgcctgacg gcgcatcaac tttatgtcta tgatgtgctt caataatttc
    13381 gatatcggtg tagtcaccca tcactttggc tgctttctcc agcagcttaa gcatgacgtt
    13441 aacgccaacg ctaaaattgg cggcaaatac aatcgcaata tcggcggcgg cgtcacgaat
    13501 cgcctgttta ccggcttcgt caaaccccgt agtgccgatc accatccctt tgccatgctg
    13561 gcgacaaaaa gcgagatggt tcagcgtacc ttccggacgg gtaaaatcga taaacacatc
    13621 aaaatcatct tttatcgcat caaggctgct ttgcacggta acgcctgttt tccctgcacc
    13681 agccagctca ccggcgtcgc tgcccagtaa agaagatcct tcgcgctcca gcgccgcgcc
    13741 cagctgcacg ccttctaatg ccagcgccgc ctgaatcaac tggcggccca tacgcccccc
    13801 ggctcccgcg atggcaacgc ggatgtttgc atcatgcata gctattctct tttgttaatt
    13861 tgcatagacc gttttcagag taaccagcgc atcgctacgc cgccagccaa aaacgctgat
    13921 aattagaaat taatgataaa cagggaggga tatcagtgaa aggcatgagt cgatgaatga
    13981 ctgcatgcca ataacatgtg acaggttacg gcgccagagc cagcacctca gccacccact
    14041 gctggaagcc ttttacattc agatccaatg ccacctgtac attggctggt ttgcccaggc
    14101 aaccgtcgat atcaaccacc gtcgtgcctg aggtaaattc gccttgagct tctaccgcga
    14161 caaaacacgg cttgagggtg aataagtccg ggcgcaccag ccaggcgatg gcgcagagat
    14221 cgtgcattcg cagaccgctt tgcatactgc cgctacggta gtggctaaac agggcgtgga
    14281 gcatttttcc ggtgcgattc aactccggta aagtagcgag atagtcaggg gttaatattg
    14341 cctgattggt gacatccaga ccgcacatga caatttcgat gccgctacgg aagacacagg
    14401 cagccgcttc tggatcggca gcgatattaa actcggcgtt tggtgtgcag ttgccgcgaa
    14461 cggcagaacc gcccatgatc accagacgac gaatatacgg tttacattcc gggcattgtg
    14521 aaagtaacag cgcaatattg gttaacgggc cgatggccac cagggtaacg ggctctggtg
    14581 cacgcatcag ggcatcccga atcgccagaa acgccggtat cccgagtggc tttcggttgt
    14641 gctcaacaaa gtcgtagcca gccattcccg attcgccgtg cacagatgct gcatcacgcg
    14701 gtgcgcgtac cagcggcaca gcggctccct gggcgagcgg aatctccgca ttccagaaat
    14761 gcagcagttg cagggcgttg cgggtggttt tctcaaccga gacattaccc gcgacggtgg
    14821 tcatcagttg caggtcgagt tcgggggcaa aaatcgcggc ggcaatggcg acggcatcgt
    14881 caatgccggg gtcagtatcg aggaagatag gtaaacgcat gttttctcca taaaaaatgc
    14941 cggtaacaag accggcattt tcgcataact taggctgcta atgacttaat cgacttcacg
    15001 aatatcgaca cgcagctctt tcggcacttc gaaaacaatg ttttcttcac ggccttccag
    15061 cggaatggct tcaccaccgc ccagctgctg caaacgtgcc accacattct gcaccagaat
    15121 atccggagcc gatgcgcccg cagtcacgcc gacgcatttt acctctttaa cccactcttc
    15181 ctggatatct ttcgcatcgt caatcaaaaa cgcgcgtttg cccatgcgct gggccagttc
    15241 cgccagacgg ttggagttgg aggagttttt cgaaccgacc accagcacca cttccgcctg
    15301 ctctgccagg gcgcgtaccg cttcctgtcg gttagtcgtg gcgtagcaga tgtcatcttt
    15361 gcgcggaccg acaattttcg ggaagcgttt acgcagcgcg tcgatcacat cagacgtgtc
    15421 atccaccgac agcgtggttt gggtcataaa ggagagtttc tcttcgtttt tgaccgtcag
    15481 tttccacaca tcgtctggcg attcgaccag atacattccc ccttccgggt tgctgtactg
    15541 acccatcgtc ccttccactt ccgggtgccc ggcgtggccg atgagaatag attcttcgcc
    15601 acgacggctg gcgcgggcaa cttccatatg cactttggtc accagcggac aggtggcatc
    15661 aaacaccgtc aaatcgcggc ttttcgcttc gttacgaacc gcctgagaaa caccgtgtgc
    15721 ggagaaaatc aggatcgcgc cgtccggcac ttcgctaatc tgctcaataa agatagcccc
    15781 acgctcgcgc aagctatcga ccacgtagcg gttatgcacc acttcgtgac ggacatatat
    15841 cggtgcgccg taaatggcca gcgcgttttc aacaatgctg atagcgcggt ctaccccggc
    15901 acaaaaacca cgcgggttgg ccaacaggat ctgcatgtta cgcctccagt gccggatcga
    15961 tttccagcac ttcaatatca aaatgaacgg tctgcccggc cagcggatgg ttgaaatcaa
    16021 cggtaatgga gtcgccgtta atttcgcgga tcacgccagg catctcactg ccatccattg
    16081 cggtaaaaag cataattgcg ccaatttctg gctcgcctgc atccataaat tcacggcggg
    16141 agaagtactg aatcaggtcc ggtgacggca cgccaaacgc cgcatcgggc tccagcgaga
    16201 aggtggtttt atcgcccact ttcagcccca gcaggtgttg ctccagccct tcagaaagag
    16261 aagcatcgcc caggcggaac agcgccggtt taccgttgtt gcgggtagac tcggcggtgg
    16321 tgccatcgtc gagttttagc gtgaagtgca ccaggacggc gctattgctc tgtacagatt
    16381 cagacatgca ggtttgctct tatattttga ggatttttgc cggatgcggc ctacgatttc
    16441 gcagcaatct gtaggcctta tccggcaggg tttattattg ttttttcgct ttagaaggca
    16501 aaaaaccttc cagcacaatc agtgccgcac cgacacagat ggcagtatcg gcaaggttga
    16561 aggtggcgaa gtgccagtcg ccgacgtaga agtcgatcat atcgacaacg aagccgtgcc
    16621 acaggcggtc gaacaggttg cccagcgcgc cgccaataat cagcgcgtaa gcgatattgt
    16681 ttagcttctg cgtggccttc gagcgataca tcatcactgc caggatcacg ctaataccaa
    16741 tcgcaatacc ggcaaagaac caacgctgcc agccgccgct atcggcaagg aaactaaacg
    16801 ccgcgccata gttacgcgca taatgcagat taagcgacgg gaacagcggg accgtatccc
    16861 ccagagcaaa gttctggagg atcaggtatt tgctgcccag atcgataatc agcacgacta
    16921 ccacaagcca cagccagcgt agccctgttg aacagatcga ttgactcatc aggcaaactt
    16981 acgtttttca ccgtcaccgg cgacgttgct gacacagcgg ccgcagattt ctgcgtgttc
    17041 cgccaccttg ccgacatcct gggtgtagtg ccagcagcgt gggcacttct caccttcggc
    17101 tttactcaac gcgactttca gtcctttgag tacttcgctc tgctgagcat cagcaggtgc
    17161 gtcgttatag tctgcaacgg tagcgccgga ggtcaacagg acaaatcgta attcatcgcc
    17221 cagcgcggtc agtttcgccg ccagttccgg ttctgcatac aaggttactg ccgcttccag
    17281 cgagccaccc actttcttgt cggcacgcgc ttgctcaatg actttgttca cttcgccacg
    17341 cactttcaac agctcgtccc agaacgcatc gttcattgct tcactgtctg ccagaccaaa
    17401 caggccttcg taccactcac cggtgaagac gtatttttca cgttcgcccg gcagatagcc
    17461 ccacacttca tcagcggtga aggagaggat tggtgccatc cagcgcacca gcgcttcagc
    17521 gatgtgatac agcgcagtct ggcagctacg acgcgccacg ctatccgctt tggcggtgta
    17581 ctgacggtct ttgatgatgt cgaggtagaa ggaacccatc tcaacggagc agaagcgcat
    17641 cagacgctgt accacttcgt ggaaatcgta tgcttcgtac gccttgagga tgtcttcctg
    17701 tgccgctttc gcacaaccta cggcccagcg atccagtacc accatctctt ccggtttcac
    17761 catatctttt gccggatcaa aaccgttcag gtttgccagc aggaagcgcg cggtgttacg
    17821 gatacgacga tagctgtcgg ctgcacgttt caggatctcg tcagaaacgg ccatttcacc
    17881 ggtgtagtca gttgatgcca cccacagacg cagaatatcc gcgcccagtt tgttcatcac
    17941 atcctgcggc gaaacggtgt tgccgataga tttagacatc ttgcggccct gaccatccac
    18001 ggtaaagccg tgggtcagta cctgacgata cggcgctttg cccttcatcg cggtggagat
    18061 catcaaagaa gacatgaacc aaccacggtg ctggtctgaa ccttccagat acatgtccgc
    18121 tgcgtgaccg gcaaattccg gacgcacatc aacaacagaa gagtgggtag atccggagtc
    18181 aaaccataca tccagcgtat ccggcacttt cacatactgg tcagcttcgt cgccgaggat
    18241 ctctttcgca tcgagatccc accacgcctg gatgccgtca acttcaacgc gtttagccac
    18301 ttcttccatc agttcgaggg tacgcggatg cagctcttcc gtgtctttgt gcacgaacag
    18361 tgacatcggt acgccccagg tgcgctgacg ggagatacac cagtcaggac ggttagcaac
    18421 catcgactcg atacgcgcct ggccccagtc cgggatccac tgcacgcctt tgatctcttt
    18481 cagtgactgc gcacgcagac ctttctgatc catgctgacg aaccactgcg gcgtcgcgcg
    18541 gaagatgatc ggcgttttgt gacgccagca gcacggatag ctgtgctgca ttttctcaac
    18601 gtgcagcagc gcgccttttt cctgcagcag cgcaacgacg atgtcgttcg ctttgaagac
    18661 gttcacgcca tccagcgtcg gataagtgcc cggcagataa gtgccgtccg ggccaaccgg
    18721 gttagcggtt tccaggccgt atttctgacc gatcacatag tcgtccgggc cgtggccagg
    18781 cgcggtgtga acggcaccgg taccggcatc cagggtaaca tgatcgccga ggattgccgg
    18841 gacgtcgaag cccataaacg gatgggcaaa gcgcagcagc tcaagctccg caccttttac
    18901 cgtgccgaga atggtgtaat cggtcacgcc gatacgctgc attacgcttt caaccagatc
    18961 tttcgccaga atcacggcct gaccgtcgat ctgcaccaac gcatagtcga aatcaggtgc
    19021 aatagagatt gcgcggttcg caggcagagt ccacggcgta gtggtccaga tcaccagcga
    19081 gattgggccg ttaacgttgc ttacgccaaa tttcgctttc agcgcatcct gatcgaccgc
    19141 ctggaaagca acgtcaatgg acggagaagt tttgtcgtaa tactcaactt ccgcttctgc
    19201 cagtgcagaa cggcagtcta cgcaccagtg caccggcttc gcgcctttgt gcaggtgacc
    19261 attgccgatg attttgccca gcgcgcggat gatattggct tcagttttga agtccatggt
    19321 caggtacggg tgcgaccagt cgcccagcac gcccagacgg ataaagtctt tgcgttgacc
    19381 gtcaacctgg gtcgccgcgt attcacggca cttggcgcgg aactcggcgg cggtgaattt
    19441 ctcacccggc ttaccgtatt cttgctctac tttcagctcg atcggcagac cgtggcagtc
    19501 ccagccaggc acatacggcg agtcataacc ggaaagccct ttggacttca cgataatgtc
    19561 tttcagaatc ttgttaaccg agtgaccaat atgaatgctg ccattcgcat aaggagggcc
    19621 atcatgcaga atgaaggttt ttttgccttt tttagccgca cggatgatgc cgtacagatc
    19681 atcatcagtc caacgcgcca gcattcccgg ttcgcgcttg gcgagatcgc cacgcatcgg
    19741 gaaccctgtt tccggcaaat tcagggttga tttatagtca ctcatcagat tctcggttcc
    19801 gtgtttcggt ttgattacat aacaggctta agccggtttt gttagcccaa aaaattcgcg
    19861 ggcggttaat tcatcacgcg caatctgcgc tttcagttcg tccagcgacg caaatcgctg
    19921 ctcattgcgt atttttttac gcagcactac ttgtatatgg cgaccgtaaa ggtccattgc
    19981 aacatctaac aaatgcactt ccagctgctg gcgaataccg gcaaccgttg ggcgtgttcc
    20041 gatgtttgcc acgccgggta acggcttttc accgaggccc agcacttcta ccgcataaac
    20101 ccctttcacc ggggaaacct gacggcgtag cggtacattc gccgtcggga aacctatagt
    20161 gcgccctaat tcatcaccgt ggactacacg cccggagatg gcaaacgggt gccccagtaa
    20221 actttctgcc agagccagat tgtcatcagc cagcgcctga cgcacggcgg tgctgctgat
    20281 acgcacacca ccttcgcaaa aagtttgcgt actggtgata tcgaagccgt attccatgcc
    20341 agctttctgt aataacaaga aatcgccttc acgaccagcg ccaaagcgga aatcatcacc
    20401 tacggcaaga aattttacgc gcaaatgctt caccaaaaga tcgctgatga aattttgcgc
    20461 ggttaacgcc gcaaaacgcc tgtcgaaacg cacgcacagc acgtaatcaa cgccacactc
    20521 tgcaaggtaa cgcagttttt cccgcagccg ggtcagccgt gccggggctt tatcggtagc
    20581 aaacagttcc agtggttgag gttcaaaaag catcaccatc accggtaagt tgcgcttgcg
    20641 cccttcttcc tgcaagccct gtaacagcgc gcgatgaccg cgatgcacgc cgtcgaaatt
    20701 accaatagtc agcacacacc cttcttgcgg ggcctggctg agattatgta tgccgcgtat
    20761 cagcttcatg tctggctcaa aacagtgaaa atcgtccgag tataccttgt acagcggtca
    20821 aggttaaccg gcgattgagt accgaaatca gaaagaagtg gcttttcatt gccatggcgc
    20881 aaatcacggg aagaaactga ccgcctgctg caatttttat cgcggaaaag ctgtattcac
    20941 accccgcaag ctggtagaat cctgcgccat cactacgtaa cgagtgccgg cacattaacg
    21001 gcgcttattt gcacaaatcc attgacaaaa gaaggctaaa agggcatatt cctcggcctt
    21061 tgaattgtcc atatagaaca catttgggag ttggaccttg gctaatatca aatcagctaa
    21121 gaagcgcgcc attcagtctg aaaaggctcg taagcacaac gcaagccgtc gctctatgat
    21181 gcgtactttc atcaagaaag tatacgcagc tatcgaagct ggcgacaaag ctgctgcaca
    21241 gaaagcattt aacgaaatgc aaccgatcgt ggaccgtcag gctgctaaag gtctgatcca
    21301 caaaaacaaa gctgcacgtc ataaggctaa cctgactgca cagatcaaca aactggctta
    21361 atcgccaatt tgctgaagct ttgtgaaaaa gcccgcgcaa gcgggttttt ttatgcctgc
    21421 tgcttttgtg atgcgtgaga gaacacactg ccggttgcca gatagtcaaa agttgcaggt
    21481 tcttgcagac atgccgttgc actatctgaa ctcttcgtgg ctaaagaatc gtagaaccat
    21541 cggctgatac attctgaaca agaatatcta ctcaatctcc agactaccct acaaagaatg
    21601 tggatattgt gatacacatt gaggtattaa acttaagacc actctatttt tgaattaaat
    21661 tcctatatac ttta
//
LOCUS       DAZFKF010000082        21608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFKF010000082 DAZFKF010000000
VERSION     DAZFKF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.082"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..825
                     /locus_tag="ACHKA1_003648"
     CDS             <1..825
                     /locus_tag="ACHKA1_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5681245.1"
                     /translation="GQGGIPALRQVPFNIRVHAQVSGGAAGWVGEGKAKPLTKFDFES
                     ITFSHAKVSAIAVLTEELIRFSSPAADALVRNALAEAVVARLDTDFVDPKKAAVADVS
                     PASITHDVKGTASTGNPDADAEAAFGQFVAANLQPTGAVWLMSSTNALALSMRKNALG
                     QKEYPDMTLLGGSFQGLPVIVSQYVGDQMVLVNAPDIYLADDGGVAVDMSREASLEMQ
                     SEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWRRRRTAAVAVITGVNYGSASGS"
     gene            870..1091
                     /locus_tag="ACHKA1_003649"
     CDS             870..1091
                     /locus_tag="ACHKA1_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681246.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            1037..3298
                     /locus_tag="ACHKA1_003650"
     CDS             1037..3298
                     /locus_tag="ACHKA1_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5681247.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLVGLPPVYAAGLAATQGHHIQANSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASTGKTVSSQLPDGASDGNKAISETEHDAVKAMFRG
                     DTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMASQAQAFEEKLTSLSAVLQKHVT
                     VDEVRLVLQAMVDDAVGAIPVPRDGRDYDPEVLKQAVNDAVANIPVPADGKSITPDDV
                     RPMLEQMVKEAVSHIPAPRDGRDYDPDVLRKAVLEAVNALPAPQDGRDATALEVLPAI
                     DDQKSFPRGTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQS
                     SGQRTEKTFSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWT
                     LAAKRGRDAGGGK"
     gene            3295..3621
                     /locus_tag="ACHKA1_003651"
     CDS             3295..3621
                     /locus_tag="ACHKA1_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO5681248.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            3631..3981
                     /locus_tag="ACHKA1_003652"
     CDS             3631..3981
                     /locus_tag="ACHKA1_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO5681249.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDCILNVVGVPVPDAT
                     GGRLEILCRLGGEK"
     gene            3978..4424
                     /locus_tag="ACHKA1_003653"
     CDS             3978..4424
                     /locus_tag="ACHKA1_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO5681250.1"
                     /translation="MIETLLDFSGLEEISRNLQLLSGAENNRVLREATRAGANELKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            4421..4765
                     /locus_tag="ACHKA1_003654"
     CDS             4421..4765
                     /locus_tag="ACHKA1_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO5681251.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            4832..5548
                     /locus_tag="ACHKA1_003655"
     CDS             4832..5548
                     /locus_tag="ACHKA1_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO5681252.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFGKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EQPQSITSDACTVTVNGAGG"
     gene            5554..5928
                     /locus_tag="ACHKA1_003656"
     CDS             5554..5928
                     /locus_tag="ACHKA1_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO5681253.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWRNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            6024..6233
                     /locus_tag="ACHKA1_003657"
     CDS             6024..6233
                     /locus_tag="ACHKA1_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO5681254.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            6281..9523
                     /locus_tag="ACHKA1_003658"
     CDS             6281..9523
                     /locus_tag="ACHKA1_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5681255.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AVAREQRRALAELNSQLTEIRASAAGTAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSAGDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGVLRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGVLNGVAGNIDNVATAAGALVAVGVARYFGNMASGAVSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAART
                     AAQNALNSTTAVGSRLMTGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALAEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLSQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERARLGYAADDLGFVGDSYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTAQGELKT
                     LTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARE
                     RMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQARAQNAQYLSERLKDQAAFYAES
                     DAQRADWQKGLQEGLSNWVDNASDYASQAAQLATEGISGLVNNITEMLNGNKVEWRNW
                     ASSVLQEISKVLMNAAIVNGIKTAANSMSGAGGFIGSIGDWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            9516..9857
                     /locus_tag="ACHKA1_003659"
     CDS             9516..9857
                     /locus_tag="ACHKA1_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5681256.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEPTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            9857..10555
                     /locus_tag="ACHKA1_003660"
     CDS             9857..10555
                     /locus_tag="ACHKA1_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5681257.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRQYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAM
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            10566..11309
                     /locus_tag="ACHKA1_003661"
     CDS             10566..11309
                     /locus_tag="ACHKA1_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5681258.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFAHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            11306..11887
                     /locus_tag="ACHKA1_003662"
     CDS             11306..11887
                     /locus_tag="ACHKA1_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5681259.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEPLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGKVVVIGRQG"
     gene            12231..15761
                     /locus_tag="ACHKA1_003663"
     CDS             12231..15761
                     /locus_tag="ACHKA1_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5681260.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETVLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPTSVRLLIQLERGGKWMTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRVLSIDAATRTLTLD
                     REVTLPGTGASTVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGTLNSVIPPAVQHLT
                     VEVSAADGQYLALAKWDTPRVVKGVRFSLRLTSGSGENSRLLTTAITADTEHRFSGLP
                     PGEYTLTVRAINSYGQQGEPATTTFRINAPAAPAGVELTPGYFQITAVPRLAVYDPTV
                     QFEFWFSEAKIADAAQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAF
                     VEARGQPSNDAAGYLELFREKIGKTHLAEALWAEIDNSQLKDEMAEMQTTITETRNEI
                     TQTVSKTLEDQSATIQQIQRVQTDTNNDLNSMWAVKLQQMQDGRRYIAGIGAGIENTP
                     DGMQSQILLAADRVAFINPENGDTTPALVTQGGQTFINEALIKTLTAPTITSGGNPPA
                     FSLTPDGRLTARNADISGNISASSGTLNNVTIAEDCTINGTMSADRIIGDIVKGYTVD
                     MGQGVMTQNQLTFQKMILLKDNMPFDRKLMLMAYSFSRGEFIMKINDKVVVHARSGKV
                     KTGEREVQISKNASRYEPVYSDVGGSASGSWSIPAGQGDVRVEITFRYEADVLFYMGG
                     SILHDKSKIRIHDPVLFYLKSYTDDFDTAS"
     gene            15946..17436
                     /locus_tag="ACHKA1_003664"
     CDS             15946..17436
                     /locus_tag="ACHKA1_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO5681261.1"
                     /translation="MTVRISGVLKDGTGKPVPGCTIELKARRTTETVIVTTVAQGQPG
                     ETGSYSFDVEPGWYRVTLNTEGYAPSYVGDILVKADSEPGTLNKFLMEQDEAQYYPKA
                     LAELEAVAAEILKRAEASAASAEEAKKRAENARGPAGEKGDTGPQGATGAQGPAGATG
                     AVGPKGEPGPKGERGETGPQGPKGDKGDPGGPPGPKGDTGPRGEAGPPGPQGPAGQTG
                     PKGDKGEPGATGPAGPAGPRGETGPAGPAGPAGSVASVPDASTSQKGVVQLSSDTNSD
                     DETKAATPKAVKAVMAEVQAAKTKAEEAATRAAVPGPKGDRGEPGAPGAVGPAGPRGP
                     AGAPGPKGDAGPAGPAGKDGTAGAEGKAGPAGPRGERGPAGAQGVPGPVGPAGPAGKT
                     GPHGPQGPTGRLVPGEIYSIGTYIIAALAPYITDIGRTYQPGETVSGSRLKRCALIKD
                     ENGNYMKANTDGIDGNLSSVPGSWMVCNEITSTNDNEGIGLFQRAY"
     gene            17456..18031
                     /locus_tag="ACHKA1_003665"
     CDS             17456..18031
                     /locus_tag="ACHKA1_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO5681262.1"
                     /translation="MWKKISNPQWADKDHTAVNCMVKFEHIEQAVPFTATASDTEAYG
                     RDIYAACLRGEAGEIAEYVQPSISPEKARELKTAGINAWRNAMEAANYTFEHNGRKWD
                     YGKSTQARLEPSVAAAKAGKLPEQFFWTDADNNDVPMDAETLIALSAAAEQAMFTKGM
                     EIHVQQRNMKKALEALNSVDEILAYKVGQES"
     gene            18510..19751
                     /locus_tag="ACHKA1_003666"
     CDS             18510..19751
                     /locus_tag="ACHKA1_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFO5681263.1"
                     /translation="MNKKFTKTILSAAVAGLFLAGNAFAQSENGIKKYSVKNAKGEHN
                     GVYELYVKEDGSAYIKWGGQAYNHNNKGKMAIDGIDADGSVRVVSADGASVYHVYDPD
                     LHSALKNIKENKTFDLREQSVGNLINKISHIKESSVNVGLDKYGQLATSPEQIKERMS
                     LQSYLASEAANSTIGLDKDGKLTEDEKNIVTPLRINDGVVTVANELDAQKGKLDAQKG
                     ELEAQKKNLGELTTRTDKIDAAAAATAAKVESRTLVGVSSDGTLTRAEGAKNTISVND
                     GLVALSGRTDRIDAAVGAIDGRVTRNTQSIEKNSKAIAANTRTLQQHSARLDSQQRQI
                     NENHKEMKRAAAQSAALTGLFQPYSVGKFNASAAVGGYSDEQALAVGVGYRFNEQTAA
                     KAGVAFSDGDASWNVGVNFEF"
     gene            19842..20312
                     /locus_tag="ACHKA1_003667"
     CDS             19842..20312
                     /locus_tag="ACHKA1_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681264.1"
                     /translation="MKVILATRNRYLEYGLQQLLKEHSVILAREFFLPENRRYIPDFD
                     ESWLIISDGLLGRLMRCMFQGRHFLQLDAELLRDGEQISDAIHNGVWTYNSAARPLTM
                     SEMVVMFGYVYRQSRPCRLASEMGINTKTVNTFLYTGMAKNGLYGVSVRRLVGA"
     gene            20586..>21608
                     /locus_tag="ACHKA1_003668"
     CDS             20586..>21608
                     /locus_tag="ACHKA1_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO5681265.1"
                     /translation="MNRIYSLRMNDRKELVAVAETAGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSYPSLASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGRNGAVGGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAG
                     AYAWKTGGTVGSPLISDWSLVSNPGYLYQSVNGPLASYGTPGDSGSPLFAWDAVKKQW
                     VLVAVLNGYAGEKGKTNWFTVIPAGDVNNTIKQDSSGTVVPAVAGGDIVWNYSKGSGE
                     GTLSQDG"
BASE COUNT         5097 a         5160 c         6965 g         4386 t
ORIGIN      
        1 ggtcagggcg ggatccctgc acttcgtcag gtgccgttca atatccgtgt gcacgcccag
       61 gtgtccggcg gtgctgccgg ctgggtgggt gagggtaagg caaaacccct gacgaagttt
      121 gattttgaat ccatcacctt cagtcatgcg aaggtgtcgg ccattgcggt actgacggaa
      181 gaattgatcc gtttttccag tccggctgct gatgcactgg tccgtaatgc gctggcggaa
      241 gcggtggtgg cgcgtctgga tacagacttt gtggacccga aaaaagccgc agtggcagat
      301 gtctccccgg cgtccatcac ccatgatgtg aagggcacgg catcaaccgg taacccggat
      361 gcggatgcag aggctgcgtt tggacagttt gtggcagcaa acctgcagcc caccggtgcg
      421 gtctggctga tgtccagcac caatgccctg gcactgtcca tgcgtaaaaa tgcgctgggt
      481 cagaaggaat acccggacat gaccctgctg ggtggctcct tccaggggct gccggtgatt
      541 gtctcccagt acgtgggtga tcagatggtg ctggtgaatg ccccggatat ttatctggcg
      601 gatgacggcg gcgtggcagt ggatatgtcc cgcgaggcat cactggaaat gcagtctgag
      661 ccgaccggcg acagtaccac gccgtccccg gtggagctgg tttccatgtt ccagacaggc
      721 agcgtggcca tccgtgcgga gcgctggatc aactggcgtc gtcgccgtac cgcggcggtg
      781 gcggtgatca ccggagtgaa ctacggcagt gcgtccggca gctgagtctg atgaggagga
      841 cgggaggcgt gcgcctcccg taacaggtta tggcaaagat ccgatatctg cagggcacgc
      901 atgatgcccg ggccggggat atccgtgatg tggcacagcc gtgtgcggag gtgctggttc
      961 gcctggggaa ggcagagtac atcacagtgc gacgtccggc aggtcagaaa aagaaacgtg
     1021 atgcggagca tggcgaatgt ggaacctttt acggcgaacc cgaaaaaacc agaaatcagg
     1081 acgtgacgta agagaggcgg gctggaccag cctgtttcag gcggtggctg agcccttttc
     1141 cggcgcctgg cagcagggcg tgaaagccga tcctgaagcc gtcctctcct ttcatgcggt
     1201 gtttgcatgt atttcgctga tatcccagga tatcgccaaa atgcggctgc gtcttatgca
     1261 gacggatgcg catgggatac gcagggaaac gcgccggggg gatattgccc gcctctgtcg
     1321 tcgtcccaac gcccagcaga accgcatcca gttttttgaa ctgtggctga acgccaaact
     1381 gcgtcacggc aatacggtgg tgctgaaaat ccgtaatgcc cgggggcaga tcaaagaact
     1441 gcgtattctg gactggagcc gggttgaacc tctggtggcg gatgacggcg aggtgttcta
     1501 tcgcattaca ccggaccgga actgcgggat cacggaggcg gtgacggtgc ctgccaggga
     1561 agtgatccac gaccggttta actgtttttt tcatccgctt gtggggctgc cgccggtgta
     1621 tgctgccggg ctggcggcca cgcaggggca tcatattcag gcaaattcaa cgtctttttt
     1681 cagaaatggc ggcaggccgt ccggggtgat tgagatcccc ggcagtataa cggaagaaaa
     1741 tgcgaaaaaa ctgaagagca actgggacag cgggtatacc ggcgaaaatg cggggaaaac
     1801 ggcgatcctg agcaacgggg cgaaatataa ccccacgacg ttttcaccgg tggatgcgca
     1861 gacggtggaa cagctgaaaa tgacggctga aattgtctgt tcggtgttcc gtgtcccggc
     1921 ctacaagatt ggcgttggcc agccgccttc cagtgacaac gtggaggcgc tggagcagca
     1981 gtattattcc cagtgtctgc agacgctgat tgagtccatt gagctgttac tggatgaggc
     2041 gctggaaaca ggggaaaacg agagtacaga atttgatgtc accacgctgc tgagaatgga
     2101 cagtgagcgg cgcatgaaaa cgctggggga tgcggtgaaa aatacgctgc tgacgcccaa
     2161 tgaggcccgt aaacgggaga acctgccgcc cctggccggc ggtgatgcac tgtatcttca
     2221 gcagcagaac tacagtctgg aggcgctgtc ccgtcgtgat gcccgtgagg atccgttcgc
     2281 gtcgaccggg aaaacagttt catcacaact gcctgacggc gcatctgacg gtaataaggc
     2341 cataagcgaa acagagcatg atgcagtgaa agcgatgttc aggggggata ctgagaaaat
     2401 gacggaacgg gaactgtcca ttattcgtgc actgggagaa gaattctcca cagtgctggc
     2461 ggatttacag cgcacatttg aggggaagat ggcctcgcag gcacaagcgt ttgaagagaa
     2521 actgacttcc ctgtcggcgg tattacagaa gcatgtgacg gtggatgagg tgcgtctggt
     2581 tctgcaggcg atggtggatg acgctgtggg ggccattccg gtaccgcgtg atggtcgtga
     2641 ttacgatccg gaagtactga agcaggcggt gaatgatgcg gttgcgaata ttccggtacc
     2701 ggcggacggc aaaagtatta cccccgatga tgtgcgtccg atgcttgagc agatggtgaa
     2761 agaggccgta agtcatatcc ctgctccgcg tgacggtcgt gactatgatc cggatgttct
     2821 gcggaaggcg gttctggagg cggtgaatgc cctgccagct ccgcaggacg ggcgtgatgc
     2881 cacggcactg gaagtgctcc ccgccattga cgatcaaaaa tcctttcccc ggggcacgta
     2941 tgccacacac cagggcggac tctggcgggc gtatgaaaaa acgcacggga tgcggggatg
     3001 ggaatgcctg gttgacgggg tggcggatat tgacgtcagc atgacgggtg aacggttgtt
     3061 ctctgtggtg gtccggcaga gcagtggcca gcgtacggaa aaaacatttt ccctgccggt
     3121 gatgctctac cgcggtgtgt tcagagccgg tgaaacctac caccccggcg atacggtgac
     3181 gtgggggggc tcgctgtggc actgcaacag tatgaccgaa gataaacccg gagaagctca
     3241 ttcatcagcc tggaccctgg ctgcaaaacg tgggcgggat gcaggaggtg gaaagtgaca
     3301 gcattactga cactggaaga aatcaaggct catctgcgtg ttgaccatga cgcggatgat
     3361 gacatgctga tggacaaggt tcgtcaggct accgccgtgc tgctggccta cattcagggc
     3421 agccgggata aggtaatccg tgaggacggt gaactgatcc cgggcgaggc attaacccgg
     3481 atgaaggggg ctgccatgcg cctgaccggg atgttgtacc ggaatccgga tcttgctgag
     3541 cgggaagacc tcgtgcaggg ggaactgccg ttttctgtgt ccgtgctgat ttacgatttg
     3601 cgttgtccga cggtgttatg aggaggggga atggcaatat ctgcaggtcg tctgacacag
     3661 atgataagtg ttctgaaccc ggtgttaacc cgtaatgctg ccggagaaat gacggaagaa
     3721 tgggtgtcat gcgggaaaat tcatgcggat atccggggca ggagcagccg ggagcggatg
     3781 cagtccggtg cggaaatggc gcaggcggaa atccgcatct gggtgcgcgg tcagtccggt
     3841 cgggaaatca cggcggcgtc acgacttcat gtgctgagtg gtccctggcg tgactgtatc
     3901 ctgaatgttg tcggggtgcc cgtgccggat gcgaccggcg ggcgcctgga aattctctgt
     3961 cggctgggag gggaaaaatg atcgaaaccc tgctggattt ttcgggactt gaagaaataa
     4021 gccgtaattt gcagcttctg agtggtgcag aaaacaaccg ggtactgcgt gaggcaaccc
     4081 gcgcgggggc gaatgaactg aaagaagaag tggtgtcacg ggcaccggtg cgcaggggaa
     4141 aactgcgccg caatgtggtg gtcctttccc ggcgttcccg cgatggcggg atggaatccg
     4201 gtgtgcatat ccgtggtgtt aatccggaca ccggcaacag cgataacacc atgaaggcgg
     4261 ataacccgcg caatgctttc tactggcggt ttgtggaaat ggggaccgtg aatatgccac
     4321 cgcacccgtt tgtgcgcccg gcgtttgatg tgcgcagtga acaggcagct caggtggcga
     4381 ttgcgcggat gaaccgggcc attgatgagg tactgagacg atgacggagg cggatttgta
     4441 tcctcatctg gcgcatcttg ccggcgggca ggtgtacccg tatgtggtcc ccctgctgga
     4501 tggcaggccg tcggtggcgc ttccgtgggt ggttttcagc ctgatttcat cggtgtctgc
     4561 ggacgtgatg ggcgggcagg cggagtcctc agtgtcggtg cagatagacg tttatgccgg
     4621 gactgtgacg caggcgcgtc agatacgtca ggacgcccgt gaagccataa tgctgctggc
     4681 cccgggatcc gtcagtgaaa tgcaggacta tattccggaa aaccgctgtt accgtgcaac
     4741 cctggagttt caggtcacgg tgtgactttt tctttttttc tacaaaaccc ataccccgcc
     4801 gcgtgcgggt tttttattat caggaggcag aatgtctgct ttgtatgaac gctcacagct
     4861 gacgcaggtg atgatttcat ctgccccggc gactgctgaa actatggata aggcggaata
     4921 tctgcgcctg gactgcacca tcaaggaagt ccagttcacc gccggtcaga aacaggatat
     4981 tgatgtgacc acgctctgct ccacagagca ggagaacatc aacggtctgg gggcgtcgtc
     5041 cgagatttcc atgtcgggta atttttatct gaatcaggcc cagaacgccc tgcgtgatgc
     5101 ctatgacaat gacgcgttgt atgcgtttaa ggtgcagttt ccgtccggta agggctttaa
     5161 gttcctggcg gaagtgcgtc agcacacctg gtcatccggt accaacggcg tggtggcagc
     5221 aacgttttca ctgcgtctga aaggcaaacc ggtgtccttt gtggtaccgc tggcgtttgg
     5281 gaaaaatctg gataagacac ttaccgtgaa taccggtgcg ctgctgacaa tgtcagtcag
     5341 tgccaacggg ggaacgccgc cgtataaata cgcctggaag aaggatggtc agccggttga
     5401 cggtcagacg acagacacct tcagtaagcc aggtgcgcag tccgctgatg cggggaaata
     5461 tacctgcgtg gtaacggatt ctgcagaaca gccgcagagc attacctctg atgcgtgtac
     5521 agtaacggtt aatggtgcgg gcggataagg cttatggcaa aagatctgaa aacactggcg
     5581 ctggccagac tgtcggggtt ccgtcataaa acggtgaagg tgccggaatg gagaaatgtc
     5641 agcgtggtgc tgcgggagcc ttcggcagag gcctggtatc tgtggcagga agtgctcaat
     5701 ggtgatggag aggatgacga taccctgtcg gtggtggcga aaacccgccg taacctggaa
     5761 gcggatgtga cgctgttctg cgatgtcctg tgtgatactg acctgcaacg ggtgttcact
     5821 ccggacgacc gtgagcaggt gctggccgtc tatggtccgg tacatgcccg gttgctgcgt
     5881 caggcactgg aactgatcgc tgatgcagag tcggccagaa aaaagtagcc cgcccggaaa
     5941 ttcgctttct gatgcgactt gggctccgtc tggggcgcac cttatccgaa ctgcggcaca
     6001 gcctgagtgc gagcgaggcg atgatgtgga tggagttcga cagggtatcc ccgctgggtg
     6061 atgagcgcgg ggatatccgt aatgcacaga tcgtgaaagc ggtttttggg gcacagggga
     6121 tgaatgttgc actgaaggac gccatgctct gctggggcga ggatgaggat aagccggagg
     6181 tggatccgtt tgcggcgctg gaagacgcgc tgagctttgc agcacagtca tgaatgatga
     6241 gaaccgctga ggcggttttt ttacgcccgg agaaaggtga atggcgacgt tacgtgaact
     6301 gattatcaaa atttcggcaa attcgcagtc attccagtcg gagatccagc gggcttcccg
     6361 catgggcagt gaatattacc ggaccctgca gaatggcggg cgtcaggctg ccgcagtcgc
     6421 ccgggagcag cgacgcgccc tggctgagct gaacagccag ttgacggaaa tccgcgcttc
     6481 agctgccgga acggcggggg catttgcagg tgcctttgcc accggacacc tgatttctct
     6541 ggccgatgaa tggagttccg tgaatgcccg actgaaacag gcgtcgcagt catccgatga
     6601 attttcgtca tcacagaaag tgctgatgga tatcagccag cggacgggca cggcattttc
     6661 agataatgcg gccctgtttg cccgctcggc agcctcaatg cgtgaatatg gctacagtgc
     6721 cggtgatgta ctgaaggtga cggaggccat ttccacgggg ctgaaaatct ccggtgccag
     6781 tacggcagag gcgggctcgg tgatcaccca gttcagccag gcgctggcac agggtgtgtt
     6841 acggggtgag gaatttaatt cggtcaatga aagcggagac cggatcatac gtgcactggc
     6901 tgcgggtatg ggcgtggccc gtaaagacct taaggcgatg gcggacgacg gcaaactgac
     6961 ggcggataaa gtcgttcctg cgttaatcag ccagctgggg gtattgcgtg atgaatatgc
     7021 cgccatgccg gaaacggtct ctgacgggat cacaaaggtg gaaaacgcct ttatggcctg
     7081 ggtgggtggc gcgaatgaag ccagcggagc gacgaaaacg ctctccggcg tgctgaacgg
     7141 tgttgccggt aatattgata atgtggcaac agcagcgggg gcgctggttg ccgtcggggt
     7201 tgcccggtac tttggcaata tggcctccgg agcggtgtct gccacggcag gacttgtgac
     7261 ggcagcacgt aatgaagtgg cactggcgga agcacagctc agggggacgc agattgccac
     7321 ggcgcgggca agggcagccg tgtaccgtgc acagcaggct gtggcggcag cccgcgggac
     7381 ggagatgcag attgctgcag aagcccgtct ggcggccaca caggaacgcc tgaacagaaa
     7441 tattgctgcc agaaccgcag cccagaatgc gctgaacagt acaacggcgg tgggctcacg
     7501 tctgatgact ggtgcgttgg gactggttgg tggcgtaccc ggactggtga tgctgggggc
     7561 agcagcatgg tatacgctgt accagaatca ggagcaggcc agggagtcag cgcgccagta
     7621 tgcactgacg atagatgaaa tcgcgcataa aacgccgtca atgtctttgc ctgaagcctc
     7681 agataatgaa ggacgaacac gggcggcgct ggcagagcag aaccggctga ttgatgaaca
     7741 ggccagccgg gtgaaatccc tgcaggaaaa aatcgctgga tatcagtatg ttctggctaa
     7801 ccctggctgg acaaccggtg acggattcat gataaaccat ctgacatcgg tgaagaccgt
     7861 aacggaaggg ctttctcagg caacagagca gcttgccgtt gagcagtccc gtctggcaca
     7921 gatgcaggaa aaagcgcagt ccattcagga tgtgcttgcc gggctggaag accgtcgtgt
     7981 ggcgttaatt cgtcagcagg cggcagagca gaataaggtg taccagtcca tgctggttat
     8041 gaacggtcag catacggaat tcaaccgtct gctggggctg gggaatgaac tgcttcagca
     8101 gcggcaggga ctggtgaatg taccgttacg gctgccgcag gccaccctgg atgataaaca
     8161 gcagagcgcc ctgacaaaaa cagaacgtga gctggccctg tcccgactga agggggagga
     8221 aaaagagcgt gcccggctgg ggtatgcggc ggatgacctt ggttttgtgg gggattcgta
     8281 tcaggaggcg agacagcgtt atatcagtaa tgctctggaa gcctggcgta ataacgaggc
     8341 gaacaaacct aaatcccggg gtggaaaatc agagacggaa aaagcggaag acagtttttc
     8401 ccggctactg aagcagcaga aagagcaact ggcactggcg ggtcagaata cagagctggc
     8461 gaagctgaag taccagactg cgcagggcga actgaaaacc ctgacggaga tgcagaagca
     8521 ggaactgctg cgtaacgcgg ccctgattga ccagcaaaaa atccgggaac agttgcgatc
     8581 ccgggaagag accctgaaga atgataatgt ggctgcgcgt gcatcaaatg aagctgaact
     8641 gctggggtac gggcagggag aacgagccag ggaacgcatg cgggagttgc agcagatccg
     8701 cgacagcttc cgccagaagg atgcggacct tcagtctcag tatcagaccg gggatatcag
     8761 tgaggatttt tacagacagg cacgggcaca gaacgcgcag tatctgagcg aacgccttaa
     8821 ggaccaggca gccttttatg ccgaatcgga tgcgcagcgt gcggactggc agaaaggctt
     8881 gcaggagggg ctcagtaact gggtggacaa tgcatccgat tacgcctctc aggcagcaca
     8941 gctggcaacg gaggggattt caggactggt gaataacatc acggagatgc tgaacggaaa
     9001 caaagtggaa tggcgcaact gggcctcatc agtgctgcag gaaatatcaa aagttcttat
     9061 gaatgccgcg attgtcaacg ggatcaagac ggcggcaaac agtatgtccg gtgcaggagg
     9121 atttattggc agtattggtg actggctggg cggtgcggtg gccaatgcaa aaggcggcgt
     9181 gtatacctcg gcaaacctga gtgcgtacag caacagcatt gtggatacgc ccacgtactt
     9241 tgcctttgca aaaggggccg ggctgatggg ggaagccgga cctgaagcca ttatgcccct
     9301 gacccgggcg gcggatggct cgctgggcgt acgcgcggtg ggcagtatga acggcagtgc
     9361 cggtctggtg tattccccgg tctaccatat cgccattcag aatgacgggg ctaacggaca
     9421 gatagggccg gaggcggcag gcagtcttgt gcagctgatt gaccagcggg tgcaggcggt
     9481 gatgctgtcc atgcgacgtg acggaggaat gctgagtggc tgagataaaa acgctgcatc
     9541 tggtcccgcg tgaagggatg caggtgagtg agaaaccgtc ggtggtgagg gttcggtttg
     9601 gtgacggtta tgaacagcgc cgaccgacgg gacttaatgc cagactgaag acgtttcagg
     9661 cggtgttccg ggtgacggat gaaccaaccc ggcgctggct ggatgaattt ttatcgtggc
     9721 atggtggtta ccgtgccttt ttgtggcgac caccgaaaca taaccggacg gtgagggtgg
     9781 tgtgccggga gtggagcgtc acagataacg ccaggtacag tgatttcagt tgtacgattg
     9841 agcaggtggt gaactgatgc aggatattca cgaagaaagt ctgaacgagt cggttaagtc
     9901 agagcagtca ccgcgggtgg tgctctggga aatcgacctg acggtacagg gcggtgagcg
     9961 gtattttttc tgtaatgagc tgaatgaaaa aggggagccg gtgacctggc aggggcggca
    10021 atatcaggcg tacccgattg aggggagtgg ctttgagatg aacggaaagg gcagcagtgc
    10081 ccgcccgtcg ctgacggtgt ccaatctgtt tggtcttgtc accggaatgg cggaggattt
    10141 gcagagcctg gtgggggcca tggtggtccg ccgccgggtg tatgcccgtt ttctggatgc
    10201 ggtgaatttc gttgcgggca atccggaggc ggacccggag caggagctga gtgaccgctg
    10261 ggtggtggag cagatgtcgg agctgacagc catgacggcc tcgtttgtgc tggcgacacc
    10321 gacggagacg gacggtgcgc tgtttcccgg tcgcatcatg ctggcgaaca cctgtatgtg
    10381 ggattaccgg ggagatgaat gcgggtataa cggtcctgcg gtggcggatg agttcgacaa
    10441 ccccaccacg gatatccgta aggacagatg cagcaagtgc atgcgcgggt gtgaactgcg
    10501 caggaatgtc ggcaattttg gcggtttcct ttccatcaat aaactttcgc agtaaatcct
    10561 gttttatgac acagactgaa tcagcgattc tggcgcatgc ccggcggtgt gcgcctgcgg
    10621 agtcgtgcgg cttcgtgata agcacgccgg agggggagcg gtatatccct tgtgtgaata
    10681 tttccgcaga gccggaggcg tattttcgta tcgcaccgga agactggctg cgggcagaga
    10741 tgcaggggga gattgtggca ctggtccaca gtcatcccgg tgggctgccc tggctgagcg
    10801 aggctgaccg gcggctgcag ataaaaagtg ccctgccctg gtggctggtc tgccggggtg
    10861 acattcacaa attccgctgt gtgccacatc tgacaggacg gcgctttgcg cacggggtga
    10921 cggactgtta cacgctgttc cgggatgctt atcatctggc ggggactgaa atgccggatt
    10981 ttcatcgcga ggatgactgg tggcgcaacg gccagaacct gtacctggat aatctggagg
    11041 ccacagggct gtatcaggtg ccgttgtcat cagcacaacc gggcgatgtg ctgctgtgct
    11101 gttttggttc atcggtgccg aatcatgccg ccatttactg tggtgatggc gagctgctgc
    11161 accatattcc tgaacaactg agcaaacgag agaggtattc cgaaaaatgg caacgacgaa
    11221 cgcattctgt ctggcgtcac cgccactggc acgcatctgc cttcacggga atttacaacg
    11281 atttggccgc cgcctcagcc tgtatgtgaa cacggcagcg gaagccatcc gcgccctgtc
    11341 gatgcagatg ccgggctttc gccgtcagat gaacgaaggc tggtaccaga tacgtattgc
    11401 cggtgatgac acggcaccgg aggcggtgta cgcccgtctt cacgaaccac tgggtgaggg
    11461 aacggtcatc cacattgtgc cgcgactggc cggggccgga aagggtggac tgcagattgt
    11521 gctgggggcg gcagccatcg tgggctcttt cttcactgcc ggggcatcaa tggcgttatg
    11581 gggttcagcc ctggcagccg gtggtttttc tgccaccacg atgctgtttt cacttggagc
    11641 cagcatgatt ctgggtggtg tggcacagat gctggccccg aaggcaaaaa caccggatta
    11701 ccgcgcaacg gataacggca gacagaacac gtacttttcc tcactggaca acatgattgc
    11761 ccagggcaac ccgatgccgg tgccctacgg tgaaatgctg gttggttcac gacgtatctc
    11821 tcaggacatc agcacccgtg atgaaggcgg gggcggaaag gtggtggtta ttggtcgaca
    11881 gggataaaac ataaaaaaat cccgcagtga tcgcggagct gcggggacag acaaatgaag
    11941 attaatgtta aggagttgtt tttgttactc gggcaaaaaa aacactaacg cagcgaaatt
    12001 atacgcgcca cagtcagttt gtgaaaatgt gaagatattc agaattttta tgccattacc
    12061 ggttttaacc aacaggatta tcggtgggca tgaaagaaaa ccccggtatc tgctgatacc
    12121 ggggtttctg tttagcatgg cagaaatgtg tttcatgctt ttcgggcgaa ggatatccga
    12181 cttctgtacg gaatggcaag tggcggttaa tttattcagg ggaaggctgt atgggaaaag
    12241 gtggcggtaa ggcacacacg cctcgtgagg cgaaggataa tctcaaatcc acgcagatga
    12301 tgagcgtgat tgatgcgatt ggtgagggac ccattgaagg tccggtgaaa ggcctgcaga
    12361 gtattctggt gaacaaaacc ccgctgacgg acacggacgg taatcccgtg atacacggtg
    12421 tgaccgcggt ctggcgcgcc ggggagcagg agcagacacc accggaaggc tttgagtcct
    12481 ccggagctga aactgtactg ggtgtggaag tgacgaaggc aaaaccggtg acacgcacca
    12541 ttacgtcagc gaacattgac cgtctgcggg tgaccttcgg ggtgcagtca ctggtggaga
    12601 ccacctcaaa gggtgaccgt aacccgacgt ctgtccgcct gctgattcag cttgagcgtg
    12661 gtggtaaatg gatgacggaa aaggatgtca ccattaacgg caagaccacc tcgcagttcc
    12721 tggcgtcggt gattctggat aatctgccgc cccggccctt taacatccgg atggtcaggg
    12781 agacggcgga cagcaccacg gaccagctgc agaataagac gctctggtcg tcatacaccg
    12841 aaatcatcga tgtgaaacag tgctacccga acacggccat tgtggggctg caggtggatg
    12901 cggagcagtt cggcggccag cagatgacgg tgaactacca tatccgcggt cgcatcatcc
    12961 aggtgccgtc aaactatgac ccggaaaaac gcacgtacag tggtatctgg gacggcagtc
    13021 tgaaaccggc atacagcaac aacccggcct ggtgcctgtg ggacatgctg actcacccgc
    13081 gctacgggat gggaaaacgc ctgggggcgg cggatgtgga caagtgggcg ctgtatgcca
    13141 tcgggcagta ctgcgaccag acggtcccgg atggattcgg gggcacagag ccgcggatga
    13201 cctttaatgc gtacctggca caacagcgta aggcgtggga cgttctcagt gatttctgct
    13261 cggcgatgcg ctgtatgccg gtatggaacg gccagacgct gacgttcgtt caggaccgcc
    13321 cgtcggatgt ggtgtggccg tacaccaaca gcgatgtggt ggtggatgat aacggcgtgg
    13381 ggttccgcta cagcttcagt gccctgaagg accggcacac ggcggtggag gtgaattaca
    13441 ccgacccgca gaacggctgg cagacctcca cggaactggt ggaagacccg gaagccatac
    13501 tgcgctacgg acgcaacctg ctgaagatgg acgcgttcgg ctgtaccagc cgcggtcagg
    13561 cccaccgtgc cgggctgtgg gtgataaaga cagaactgct ggaaacgcag acggtggatt
    13621 tcacactcgg gtctcagggg ctgcggcaca cacccggtga catcattgaa atctgtgata
    13681 acgactatgc cgggaccctg accggcggac gtgtcctgtc cattgatgct gccacccgca
    13741 ccctgacgct ggaccgtgaa gtgacacttc cggggacagg tgcatcgacg gtgaacctga
    13801 ttaacggcag cggtaagccg gtgagtgtgg acatcaccgc acaccccgcg ccggaccgga
    13861 tacaggtcag taccctgcct gatggtgtgg agacatacgg ggtgtgggga ctctccctgc
    13921 cgtcactgcg ccgtcgcctg ttccgctgtg tctccatccg ggaaaacacg gacggcacct
    13981 ttgccatcac ggcagtgcag cacgtaccgg aaaaagaagc cattgtggat aacggagccc
    14041 gctttgagcc gcagtcaggc accctgaaca gcgttatccc accggcagtg cagcacctga
    14101 cggtggaggt gagcgcagct gacggccagt atctggcgct ggcgaaatgg gacacgccgc
    14161 gggtggtgaa gggcgtgcgc ttcagtctgc gcctgaccag tggaagcggt gagaacagcc
    14221 gcctgctgac caccgccatc actgccgata cggagcaccg tttcagtggc ctgcctcccg
    14281 gggaatacac cctgacggtc agggcgatta acagctatgg ccagcagggc gaacccgcca
    14341 ctaccacgtt ccggattaac gcaccggcag cgcctgccgg tgttgaactg acgccggggt
    14401 attttcagat aacggcggta ccgcgtcttg cggtgtatga cccgacggtg cagtttgagt
    14461 tctggttttc ggaggcaaaa attgcagacg ccgcacaggt ggaaacctct gcccgttatc
    14521 tggggaccgg cagtcagtgg agtgtctccg gcccgcacat taagcccgga aaggatttct
    14581 ggttttatgt gcgcagcgtc aacctggtgg gtaaatctgc ttttgtggaa gccagagggc
    14641 agcccagcaa tgatgctgcg ggctatctgg aacttttccg ggaaaagata ggaaaaacgc
    14701 atctggcaga ggcgctgtgg gcagagattg acaacagtca gctgaaggac gagatggcgg
    14761 aaatgcagac caccatcaca gaaacccgca atgaaatcac gcagacggtc agtaaaaccc
    14821 tggaagacca gagcgccacc atacagcaaa tccagcgtgt gcagacggac acaaataatg
    14881 acctgaacag catgtgggcg gtgaagctac agcagatgca ggatggtcgt cgctatattg
    14941 ccggtatcgg tgccggtatt gagaacaccc ctgacgggat gcagagccag atactgctgg
    15001 cagcggaccg cgtggcattc attaatccgg agaatggtga taccacaccc gcactggtca
    15061 cgcagggggg acagacgttt atcaatgagg cgctgataaa gacgctcacc gcccccacca
    15121 ttaccagcgg cggcaatccc ccggcattct ccctgacgcc ggacggcagg ctgacggccc
    15181 gtaatgccga tatcagcggg aatatcagtg ccagttccgg tacgctgaat aacgtgacga
    15241 ttgcggaaga ctgcaccatt aatggaacga tgagcgctga ccgaatcatt ggtgatatcg
    15301 tcaagggata cacggttgat atggggcagg gcgtaatgac tcagaaccag ctgacttttc
    15361 agaaaatgat tttgctgaaa gacaacatgc cgtttgatcg caagcttatg cttatggcgt
    15421 attcattttc cagaggtgaa ttcatcatga agataaatga caaagttgtc gttcatgcca
    15481 ggtcaggcaa agtcaaaacc ggggagcggg aggtccagat ttccaaaaac gccagcagat
    15541 atgagccggt atattctgat gttggcggaa gtgcgtcagg ctcatggagt attcctgccg
    15601 gacagggtga tgtgcgggtt gaaattacat tcaggtatga agcagatgtg cttttttaca
    15661 tgggtggttc aatacttcat gataagagta aaattcggat ccatgacccg gttctgtttt
    15721 acctgaaatc ctatacagac gattttgata ccgcatcgtg acaggtggat agtgaggttt
    15781 catccatctg ttcgtgattg ccggaaaacg acaaaagccg gacatcgtac attatccacg
    15841 ggtgcctttg actgacggca ccctttaaca ccagccagtc acaatttata cccctgatcg
    15901 cagaccgggt aaccggtact gcggtttttt ttatgggaga aatctatgac agtcagaata
    15961 tcgggcgtgc tgaaggacgg caccgggaag ccggtgccgg gatgcaccat agagctgaaa
    16021 gcgcgacgca ccacggagac agtgattgtc accacggtgg cgcaggggca gccgggggaa
    16081 accggcagtt acagttttga tgtggagccg gggtggtacc gggtgacgct gaacacggaa
    16141 gggtacgccc cgtcgtatgt gggtgacatt ctggtgaagg cggattctga gccgggaacg
    16201 ctgaataaat ttctgatgga acaggatgag gcgcagtatt acccgaaagc gcttgcagag
    16261 ctggaagcgg tggcagcgga aatcctgaag cgtgctgaag cgtcggcggc gagtgcagag
    16321 gaagcgaaga aacgggcaga gaatgcccgg ggaccggcgg gcgagaaggg ggacacaggt
    16381 ccacagggtg ccacaggggc acagggacca gccggggcaa cgggggcggt cggaccaaaa
    16441 ggtgagccgg ggccaaaggg agaacgggga gaaacaggtc cacagggacc gaagggcgat
    16501 aaaggtgacc cgggcggacc gccggggccg aaaggtgaca ccggcccacg tggagaggcc
    16561 ggaccacccg gaccacaggg tccggcaggg caaaccggcc cgaaagggga taaaggtgaa
    16621 cctggcgcaa caggtccggc aggtcccgca ggccctcggg gagaaaccgg ccctgcaggt
    16681 cctgcgggtc ccgcaggcag tgtcgccagt gttccggatg ccagcacatc acagaaaggt
    16741 gttgtacagc tgagcagcga taccaacagc gatgatgaaa caaaagcggc aaccccaaag
    16801 gctgtgaaag cggtaatggc agaagtgcag gcagcaaaaa cgaaggcaga agaggcagca
    16861 acccgggcag cagtccccgg accgaagggg gacagggggg aacctggcgc acctggtgca
    16921 gtggggccag caggcccacg gggaccggct ggcgcacctg gtcccaaagg tgatgcaggc
    16981 ccggcaggcc ccgcaggaaa ggatggtacc gccggagcgg aaggtaaggc aggtccggca
    17041 gggccacgag gtgaaagggg accagcgggg gcacagggtg ttccggggcc ggttggtccg
    17101 gcaggtcctg cgggtaaaac ggggccacat ggaccacaag ggccaaccgg aaggctggta
    17161 cccggggaaa tttacagcat cggaacatac attattgccg cactagcccc gtatataacc
    17221 gacatcggca gaacctatca gccaggagaa actgtatcgg gttcacgcct taagcgatgc
    17281 gccctaatca aagacgaaaa cggaaactat atgaaagcta acaccgatgg cattgacggc
    17341 aacctatcct cagttccggg ctcatggatg gtgtgtaatg aaattacgtc gacaaatgac
    17401 aatgaaggca tcgggctgtt ccagcgcgca tactgacaga caggagatga catgaatgtg
    17461 gaaaaaaata agcaatccgc aatgggcaga taaagatcac actgctgtca attgcatggt
    17521 gaagtttgag cacattgagc aggcggttcc tttcactgca acagccagcg ataccgaagc
    17581 atacgggcgg gatatttatg ccgcttgtct gagaggagag gccggtgaaa ttgcagaata
    17641 tgtacagcca tccatcagcc cggaaaaagc cagagaactc aaaaccgccg ggatcaacgc
    17701 ctggcgtaat gcgatggaag cggcgaacta cacgtttgag cacaatgggc gtaaatggga
    17761 ctacgggaag tcaacgcagg cgcgtcttga gccgtcggtg gcggcagcga aagcggggaa
    17821 actgccggag cagtttttct ggacggatgc ggacaataat gacgtaccga tggacgctga
    17881 aacactgatt gctctgagtg ctgctgcaga gcaggcgatg ttcaccaaag ggatggaaat
    17941 tcacgtacag cagcgcaaca tgaagaaggc actggaagcg ctgaacagtg tggatgagat
    18001 tctggcgtat aaggtgggtc aggagtcgta aataaaaaac gggggtgatg cctcaccccc
    18061 tctttcgtca acagaggatt acagcaatcg tttttttatt gtgaaacaca atctgcaata
    18121 accgccaggc atattcagtg atgcctgtca cacgctccgg aacgcggatg gcaacggtat
    18181 gtcagaaaac gattaatatt attaatgcat caaaaaaaat cagaatgtgg tgcttgtcac
    18241 attattaagc gtaaggaact gtgtcataag tgcgcgaggg aaataagcga aacgacagca
    18301 tgcggtgtgg agcgtgaata atggcgggga tgacggatat caccgactgt acagtacgat
    18361 aattcctaaa aagtttatat tcatttttgc gaataatcct ggaataagct gacaggaagc
    18421 ggccagtcat cagattttct ttagtgtatc tgaagtgcgt ctgagagggt tgcttttttt
    18481 gtatacaaag aaattacagg atatatcaga tgaacaaaaa attcactaaa acaattcttt
    18541 ctgctgcagt ggcgggattg tttctggcgg gtaacgcttt tgcgcagtcg gaaaatggta
    18601 ttaagaagta ttctgttaaa aacgccaagg gtgaacataa tggcgtctac gaactgtatg
    18661 tgaaagagga tggttccgca tatattaaat ggggtggcca ggcatataat cataacaata
    18721 aaggaaaaat ggcaattgac ggtattgatg ctgatggctc tgttcgggtt gtgagtgcag
    18781 atggtgcatc tgtatatcat gtatatgacc ctgacttgca tagtgcactg aaaaacataa
    18841 aagaaaataa aacatttgac ctcagggagc agtcagtcgg caatctgatt aataagattt
    18901 cacacataaa agaatcctct gttaatgttg gtctggataa atatggtcaa ctggcgacat
    18961 ctcctgagca aattaaggag cgaatgtcac ttcagtctta cctggcgagt gaagcagcta
    19021 actcaactat tggccttgat aaagacggta agctgaccga ggatgagaag aacattgtaa
    19081 caccattacg tattaacgac ggtgttgtga ctgttgcgaa tgagcttgat gctcagaaag
    19141 gcaagcttga tgctcagaaa ggcgagcttg aagcgcagaa gaaaaatctt ggtgaactga
    19201 ccacgcgtac cgacaagatt gatgctgcgg ctgctgcaac agctgcaaaa gtcgaatccc
    19261 gcacactggt tggtgtgagt tctgatggca ccctgacccg tgcagaaggt gcaaagaaca
    19321 ccatttctgt taacgatggt ctggtggccc tgtctggtcg tactgaccgt attgatgcag
    19381 cagttggtgc cattgatggc cgtgtcaccc gtaacacgca atccattgag aaaaacagca
    19441 aggcgattgc agccaatacc cgtacgctgc agcaacattc agcacgtctg gacagccagc
    19501 agcgccagat taatgaaaac cataaggaaa tgaaacgagc agcagcacag tctgcggccc
    19561 tgactggtct gttccagccg tacagcgtgg gtaaattcaa cgcgtcagca gcggtgggtg
    19621 gttacagcga tgagcaggca ctggcagtgg gtgtgggtta ccgcttcaac gagcagaccg
    19681 cagcgaaagc aggcgtggca ttcagtgatg gggatgcctc ctggaacgtt ggtgtgaact
    19741 tcgagtttta attgctgaag gtgacatgaa gtatgacggc aggcacagga gttttgcctg
    19801 ccgttttttt tcatggggca taaaaacagg aaggtgctgc gatgaaggta attctggcca
    19861 caaggaaccg ctacctggaa tatggactgc aacagttgct gaaggaacac agtgtgatac
    19921 tggcgaggga gtttttcctg ccggagaacc gccgttatat cccggacttt gacgaatcct
    19981 ggctgataat cagtgatggc ctgctgggca ggttgatgcg gtgtatgttc caggggcgtc
    20041 attttctgca actggatgcg gagttgctcc gtgatggtga gcagataagt gatgccatac
    20101 acaacggcgt ctggacgtat aacagtgctg cccgcccgct gacgatgtca gagatggtgg
    20161 tgatgttcgg ttatgtctac cgccagtcga gaccctgccg ccttgccagt gagatgggta
    20221 ttaacacaaa gacggtgaat acctttctgt atacgggaat ggcaaaaaac ggactgtatg
    20281 gtgtgagtgt cagacggctt gttggtgcct gatggcggtc ctgccggatg cgcggcatct
    20341 tctgatgatt cagaaggtgc agatgatgaa caaagacaga gaccggagat acgggagaaa
    20401 gcgttacacc acaggcgcaa tgtgtttcat gttgtttgtg tggctgggtg gggtggtggc
    20461 tctttttacc gcggcagctg tgttgcttca gtaatctggc ggcagtattc gtcagattcg
    20521 ttaacagata tccgggcagg cgtcactgtt gactgctgtc cgggaaaaga cagggaaacc
    20581 ggggaatgaa cagaatatat tctctcagaa tgaatgacag aaaagaactg gttgcggtag
    20641 cggaaacggc gggcggcagg aaaaagtcgt ccggcattcc cggtgcgggg atgctttccc
    20701 gtcttcttct ggcctcaggt gccgttgccg gtgttctgtt ttcttatccg tcgctggcat
    20761 ccgttgtcgg gaataccctg ccctggcaga cctacaggga ttttgctgag aacaagggcg
    20821 cgtttcatgc cggtgccaca aatattccgc tttatggcag gaacggggct gtcggtgggc
    20881 ggctggacaa ggcgccgatg atggatttca gtgttgtgga ccagatactg ggggtggcaa
    20941 cactgatttc tccgcagtat gtggcggggg ttaagcataa cggcagctac aacacggtgc
    21001 gttttggtta tgcggatgat acgacatatc gccttgttga ccgtaatgag cactggcgtg
    21061 attttcatac tccccggctg aacaaactgg tgacagaagt tgccccggtg agcgtcacgg
    21121 atgccggaac cggcaagggg gtataccaga accgcagtcg ttatccggtg ttttatcgta
    21181 tggggagtgg aacgcagtac accggagctg cttccggtgc gctgaccaga atagcgggtg
    21241 cttacgcctg gaagacgggc gggacggtgg gaagtccgct catttctgac tggagtctgg
    21301 tgtcaaaccc ggggtatctg taccagagtg tcaatggtcc gctggcatct tacgggacac
    21361 cgggagacag tggctcaccg ctttttgcct gggatgcggt gaagaagcag tgggtccttg
    21421 ttgctgtact taatggttat gccggtgaga aagggaagac gaactggttt acggtgattc
    21481 ccgcagggga tgtgaataac acgataaagc aggacagttc aggcactgtc gtgccggcag
    21541 ttgccggtgg tgacattgtg tggaattaca gtaagggaag cggagaggga accctttctc
    21601 aggacggg
//
LOCUS       DAZFKF010000083        21465 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFKF010000083 DAZFKF010000000
VERSION     DAZFKF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.083"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(37..297)
                     /gene="yfhL"
                     /locus_tag="ACHKA1_003669"
     CDS             complement(37..297)
                     /gene="yfhL"
                     /locus_tag="ACHKA1_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO5681266.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            512..1909
                     /locus_tag="ACHKA1_003670"
     CDS             512..1909
                     /locus_tag="ACHKA1_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5681267.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSSRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVHEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAVKGIKHDGTVKGKDGAFNTMCRKFLKSN
                     VIALMKERGYSRSDRREISLLCLHHSSKSDDPMAEHYDFSDEILQEEIALKREAFEAH
                     ERSILAQVALLRRRG"
     gene            complement(1906..2106)
                     /locus_tag="ACHKA1_003671"
     CDS             complement(1906..2106)
                     /locus_tag="ACHKA1_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5681268.1"
                     /translation="MSEKPDDLLTPDEVCQKLGITQKTLCEWNIKHRHRAILAPIRFS
                     AKVVRYERRNVDAFIQKCRSQY"
     gene            complement(2103..3497)
                     /locus_tag="ACHKA1_003672"
     CDS             complement(2103..3497)
                     /locus_tag="ACHKA1_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFO5681269.1"
                     /translation="MGQIFTPRPYQDLIINHEIGISRCNIWAGMGMGKTVATLTTLED
                     LFMAGSETQPALVLAPLRVAASTWPDEALKWGHLRNIEVQPIVGNAKARSAALANSNA
                     SVFTINYDNLVWLVEELGERWPFGTVIPDESTRLKSFRLRGGGKRAAALGKVAHKYVQ
                     RWINLTGTPAPNGLVDLWGQLWFVDQGQRLGRTYGAFTSRWFNSIQFPGQSWTKLEPF
                     AHSQAEIQRALADVTLSLDAADWFDIKDPIHNVIRVDMPPKARQQYREMEKEMFLELN
                     GEGIEAPNAAAKTLKCLQIASGAVYTDDTGSWSELHDTKLQALDSILTEAAGAPVLVA
                     YHWKHDLERLLKAFPRGRHLDQDPQTLRDWNSGKIPVLFAHPASAGHGLNMQDGGNIL
                     VFFSHWWDLEQYQQIIERIGPTRQIQAGHNRPVFIHHIIAADTMDEMVMERRNSKRTV
                     QDILLDAMKKRGIA"
     gene            complement(3542..4321)
                     /locus_tag="ACHKA1_003673"
     CDS             complement(3542..4321)
                     /locus_tag="ACHKA1_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5681270.1"
                     /translation="MSERTLTLAGTGKQPMMSSREIAEVVNKNHADVCRDIRAMLAKL
                     YGGDERDYIRKADLLYLTNQCVNCLQYNTDNPNAWEYQLDYEHSECLVTGYDAVRRMA
                     MIKHWQKLKKEVEPPQLAITHPAPVIQVDSRIVQLARVIAEATASATMKAMSEVIALP
                     TYQAESAAPANLSHVEGEYVPVSKAAWKTGLSDSTCRKLIGFARIPVRSDTGVRGLLV
                     NLPAIEQAAQKLLNESTPPKGNRKRWTHPQFGNFTYYTDLI"
     gene            complement(4382..4558)
                     /locus_tag="ACHKA1_003674"
     CDS             complement(4382..4558)
                     /locus_tag="ACHKA1_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681271.1"
                     /translation="MAIQITQLHQADIFGYIADMLETARLLSSLEKGEQLAFELIYFA
                     QQAAREAANKPWDE"
     gene            4671..5000
                     /locus_tag="ACHKA1_003675"
     CDS             4671..5000
                     /locus_tag="ACHKA1_003675"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO5681272.1"
                     /translation="MSKRDDPQLRVRIPELLKEALEKKARANKRTLTSEIVSRLESTI
                     LQDKLLHTSRGFEEAADEILILRDLLEKLKTTYHREYQVEWVYENKYELIEVMEKLKK
                     LLNPENE"
     gene            complement(4969..5145)
                     /locus_tag="ACHKA1_003676"
     CDS             complement(4969..5145)
                     /locus_tag="ACHKA1_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO5681273.1"
                     /translation="MSKRDDPQLRVRIPQDLKDTLEKISRENDRTLTAEITRRLRKSL
                     EDESTIHSQDLATS"
     gene            complement(5142..5411)
                     /locus_tag="ACHKA1_003677"
     CDS             complement(5142..5411)
                     /locus_tag="ACHKA1_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO5681274.1"
                     /translation="MAYERESLIEKHLVAEVKKAGGVAFKFVSPGRRSVPDRIVLLPG
                     GRIVFVECKAPGKPPRPDQLREHERLRKLGFTVVVLDSKNLEGIL"
     gene            complement(5412..5651)
                     /locus_tag="ACHKA1_003678"
     CDS             complement(5412..5651)
                     /locus_tag="ACHKA1_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681275.1"
                     /translation="MRNTAKLQLGFSPLSKKIMLAKMRDVDGGRLRVGNDPGRDVTNE
                     AAQLVWQLVMAEGGEISWGLDDGSQMVLRAEKQVK"
     gene            complement(5651..5866)
                     /locus_tag="ACHKA1_003679"
     CDS             complement(5651..5866)
                     /locus_tag="ACHKA1_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO5681276.1"
                     /translation="MSFKYRDNPLYYRAAREALRLEQSGEYDRAAKVWAKANRQSRNE
                     LNQDWSERRSDFCLMQNMREKRKAVEE"
     gene            complement(5872..6075)
                     /locus_tag="ACHKA1_003680"
     CDS             complement(5872..6075)
                     /locus_tag="ACHKA1_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681277.1"
                     /translation="MTVIKTHTGTVITRTGEKQVQLHQNATTWVAGPKEYYYKDTGRR
                     GGAVGVRTRLVLSSIRPIEAKQE"
     gene            complement(6081..8144)
                     /locus_tag="ACHKA1_003681"
     CDS             complement(6081..8144)
                     /locus_tag="ACHKA1_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFO5681278.1"
                     /translation="MSNILWGDLETFCEIPINNGTHAYAEGVEVMLFAWAINDGPVNV
                     WDITAGGGIPHGLYEAIADPETLLYFHNSHFDRTVLRYAIPRLAPPVERWRDTMVQAL
                     AHGLPGSLGELCEVLGVPQDKAKDKEGKALIQLFCKPRPKNSKLRRATSKTHPEEWRR
                     FVAYAGLDIEAMREVYKRLPKWNYQGTELALWHRDQQINDRGVCMDMQLARAAIEAVD
                     QEQKRLAKRTQEMTDGEVQAATQRDALIKHIVESYGVELPDMQRSTLERRIADPHLPS
                     AVKELLAIRLQASTTSTSKYKALMKGVSHDGRLRGTLQFCGASRTGRWAGRLFQPQNL
                     PRPSLKQEQIDEGIEALKAGCADLLFDNIMELTSSALRGCIIAPTGKKLVVSDLSNIE
                     GRMLAWLAGEEWKLNAFREYDAGTGPDLYKLAYAKAFDIAPDDVDKHMRQIGKVMELG
                     LGYGGGVSAFITFALVYGLDLDELANAALPNIPRDVIREAKSWYNESVKRKSTFGLSE
                     RVFIACDSLKRLWRRAHPATCDFWYELERTVRTAIATPQKTLYCGYLKIRRDGAWLRI
                     QLPSGRAVCYPSPVIEQGNITYMGVNSYSRKWQRLKTYGGKLVENVTQAAARDVLAGN
                     MPLIEDAGYSIVLTVHDEVITESPDTEDFNDKELSALLSNNPEWAPDIPLNAGGFEAY
                     HYRKD"
     gene            8171..8770
                     /locus_tag="ACHKA1_003682"
     CDS             8171..8770
                     /locus_tag="ACHKA1_003682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681279.1"
                     /translation="MDTKDIISLVGVIISAITAPVGVYKLFKDASWFLPKTTKFSHIL
                     KNYPEHIEPSDVEFMKAEIKREVKKSILGISSQKLRTLVIYVRTYSELKMPFWQWGYL
                     APHIQCKYDRFFIRYKGKYKRCRWCSKVVSIFYLADGLIFFCWSLNYGAVFIGMGAVL
                     MLLCIWMAFMFWFLFPGRGVIKKYNSQLLKIDASKYQAK"
     gene            complement(8885..9649)
                     /locus_tag="ACHKA1_003683"
     CDS             complement(8885..9649)
                     /locus_tag="ACHKA1_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFO5681280.1"
                     /translation="MSTQIVFKSHVLETIEHNGKTWFTAATLATALEYSDARSVTNIY
                     TRNTDEFTLGMSEVIKMVTSGNLQASRRIFSLRGAHLVAMFATTPVAKEFRRWVLDLI
                     EKETAVPQSSTILAPHRECLPKMVYHHRSKYNPYRAYAWDGEKSVYVGCYPTVGEAVA
                     AQERFYRNGSTKRIQKAQPAVSDAEKEMFINNLRAVCHNFRRINEIWRAQLMPALEKM
                     DSKLVYQLHDRFSDSMCILPTIEDRIGRYIPPTLPR"
     gene            complement(10031..10579)
                     /locus_tag="ACHKA1_003684"
     CDS             complement(10031..10579)
                     /locus_tag="ACHKA1_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFO5681281.1"
                     /translation="MKIKLNNVRLAFPALFEAKTVNGEGDPRFSAVFLISPKHPQLEE
                     IRKAMKQVAKEKWGEKWESIYNQLEKKLNLCLHDGDEKAEYEGFPGNFFLNAANKARP
                     AVLDRDRSPLIQADGRPYAGCYVNAVIDIWAQDNNFGKRINASLGGVQFLRDGDAFAG
                     GGVASADDFDDISEGADAEALI"
     gene            complement(10595..11896)
                     /locus_tag="ACHKA1_003685"
     CDS             complement(10595..11896)
                     /locus_tag="ACHKA1_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFO5681282.1"
                     /translation="MPDVHARLSPSSAHRWLRCAGSLALEATQPDKETTFAIEGTAAH
                     ALAEKVLRNRQSHPEHYAGCNVSMFLGSYPLRENPDDTSGPQVDDEMVEAVGRYVDTV
                     WTLAQNNELLVEQRVDFSHITGVEESFGTADGIIIAGNELQIHDLKYGKGVRIDAEQN
                     EQLQLYALGALEQFSMLYDFETVRLFIHQPRLNHVSEWSLTVQELQSFGKRAQEAATS
                     AILVLNIAECEGIETLPLENFIPGEKQCRFCKAKAICTAQKMQHLQTAASDFEDLTKP
                     VSEIITNASARVPLLTIEQLAEIYSQADFIESWLKAVRDRVHNELNAGNPVPGFKLVT
                     GKQGNRAWSDEEAARALLKDQFRYKTEEVFDLKLISPTKAEKLIKKASPRRWSKVEAL
                     ITRADGKPTVVPESDPRPALNINPVNDFDDVSDDTLAADLI"
     gene            complement(11889..12407)
                     /locus_tag="ACHKA1_003686"
     CDS             complement(11889..12407)
                     /locus_tag="ACHKA1_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HFO5681283.1"
                     /translation="MPEAWKDISGFEGIYQISDAGNLRSLDRTVLNKGSGCTYPIKGK
                     SLKPRYDADGYLITDLWKAGKKVTVKIHRLVAAAFIPGSAPEVDHINGRRDDNRAVNL
                     RWASLSQNRANSHTRKNKSGVVGVRFSEGKKNPWQAYGRLNGKFKSLGHFPTKQLVAA
                     ARRKHIQEVMNA"
     gene            complement(12410..13312)
                     /locus_tag="ACHKA1_003687"
     CDS             complement(12410..13312)
                     /locus_tag="ACHKA1_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681284.1"
                     /translation="MSLETSLELNNQLLEQHNALLERLIRTMASGIVMHPDTISRVQE
                     CRDTATEAENMSAAMTLDDLEFSDVIALAGFYPVATPITEDMLQRAVAYRDAEGDKRV
                     VQIDALDSALQGVKRARALLKPALLDLSRNILKFWDDLPTIGERRAFAEQLLDAPADG
                     RDEVKPKKASNKDGERTGPFYVKNVSGTAASELHTLRKLNEMLKKGHIEINRVEYLQL
                     QEEFARRDAANSSQNNDAKDDHTIDFAALRKQAEGLILQLAKGGYRAEAIAILEKQGA
                     RKLGEVTDENLAEVITLAEKALEG"
     gene            complement(13309..13458)
                     /locus_tag="ACHKA1_003688"
     CDS             complement(13309..13458)
                     /locus_tag="ACHKA1_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681285.1"
                     /translation="MKPEHLYRLTGRDVLRYRRKNFDLMTGLAVATALGLIITFILLV
                     ARTTV"
     gene            complement(13560..13853)
                     /locus_tag="ACHKA1_003689"
     CDS             complement(13560..13853)
                     /locus_tag="ACHKA1_003689"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO5681286.1"
                     /translation="MAGKPAGKVKADGYVTICLDGKNQYVHRLVWEYHFGDIPAGMEI
                     DHINHNRADNRIENLRLVTRAENAKNLAQRSPTKRNGGQLERIKKALAGTNHR"
     gene            complement(14041..14247)
                     /locus_tag="ACHKA1_003690"
     CDS             complement(14041..14247)
                     /locus_tag="ACHKA1_003690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681287.1"
                     /translation="MNTYELTTKYAPAFEVVENGPDATDYVVATVKGENPARLRAAAY
                     QERNGYLGYGYTIRPLTNSSESSM"
     gene            complement(14632..15261)
                     /locus_tag="ACHKA1_003691"
     CDS             complement(14632..15261)
                     /locus_tag="ACHKA1_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO5681288.1"
                     /translation="MNLANRAKKRRTELNLTQVEVAKRAGISQQSIEAIENGKTLKPR
                     NLLALASALECDPKWLLLGGDVVTDFNYGARRVPVLSYVQAGAFTSAELLREEGDFEY
                     ILTTAELSESSFALRIRGDSMEPEFKEGDIVIIDTDVYPTPGEFVAACNGSHEATFKK
                     YRPVGIGAKGEEDFELVPLNSDYPICRSWEKPIKIIGTMVEHRIFRRKR"
     gene            15369..15593
                     /locus_tag="ACHKA1_003692"
     CDS             15369..15593
                     /locus_tag="ACHKA1_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5681289.1"
                     /translation="MTSSTLASRIKERRKALGITQTSLAESVGMRQQSVQYLESGRAT
                     RTGFILELAKVLKCDPDWLLNGENSEQQKV"
     gene            15597..17816
                     /locus_tag="ACHKA1_003693"
     CDS             15597..17816
                     /locus_tag="ACHKA1_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFO5681290.1"
                     /translation="MPDNKPWGATPDEWFHFDLVLGMTDRLLPVVSNPCAELSPNSKL
                     SHNFGKTPSRYNGQRKVVGIPDWANYVVTENDFARWSNEPDYGICVRTGDGVVALDCD
                     INDAGMQEIVRNIILSCLGELPPRRWRADSHKCLYLIAVDGDYRKRGHRLEGENKQIE
                     LLAKGQQFVACGTHPAGERIQWDCGLPGEPLKITSEQLESLWQRLADNLPVKDSYTAG
                     AGRQRDLSCVDPSATDDVADWLDANGWTLSVSSDGSRNLKPFRDESEYSNGCSETSIK
                     YFPKGTGGFELGHFKSMHNTDAGLTDADWLDAYGYRVADFEDLTVAEDGDKPEYLPWP
                     KFSRDKNDRIEGTVTNVLLALRRPDLCGVQIRLDEFRDEIILITPRGKNLRLRDEFYT
                     MLHSRLECLGFKKFEEAAIRRAVRLVAYENRFDSLKDWIANLPAWDGVPRIDTFFCRH
                     WGIEANAYTHAVGRYWWTLLAGRALVPGIKGDMAVILVSQQGKNKSEGIRAMAPTPEH
                     YMELDFGKKGEERIREMRGHNVIELGEMRGMDKRGIGDVRVTISTRADRNRGLFREHY
                     VTLPRRCGFIGTSNSDTPLTDTEGNRRWLPMTIPDETDGKFIAQGIADEKIQLWAEAV
                     AVFKRDGIAWENAETLAKSILGDYEVKDEVWMSCVAEWLELPQDLYDMAADGTKKNCE
                     RVPLTSKDVLVEAIGFKAPQVKRSDEMKIAAIMKKLGYKNKQLQSLPGKPRSWEKCK"
     gene            18123..18404
                     /locus_tag="ACHKA1_003694"
     CDS             18123..18404
                     /locus_tag="ACHKA1_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681291.1"
                     /translation="MNTTCARLPSADTLAEQAAKTASGWMSDAVIAIDELFGKGYAKQ
                     HPELVAAFMQTAGLDEAAMHLRGLVLANQRIDDTLEVLSEHIMLLNEPQ"
     gene            18473..18895
                     /locus_tag="ACHKA1_003695"
     CDS             18473..18895
                     /locus_tag="ACHKA1_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013813178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5681292.1"
                     /translation="MRRDMQLVLERWGRWAASEEYCSMVDWPSMSVTSRDVTDSGKPG
                     CSDEDGAVIDTCIAHMSMVCPYDDLLILGLRFIGGLSTRAIAEAIDRSHLSVRTSLKA
                     SEAFLEGALTLQGVRLDMDPEVVLPERVVCAQKNMLWL"
     gene            18975..19076
                     /locus_tag="ACHKA1_003696"
                     /pseudo
     CDS             18975..19076
                     /locus_tag="ACHKA1_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            19065..19141
                     /locus_tag="ACHKA1_003697"
     tRNA            19065..19141
                     /locus_tag="ACHKA1_003697"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19099..19101,aa:Arg,seq:tct)
     gene            19233..20192
                     /gene="stxA2b"
                     /locus_tag="ACHKA1_003698"
     CDS             19233..20192
                     /gene="stxA2b"
                     /locus_tag="ACHKA1_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO5681293.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            20205..20468
                     /gene="stxB2b"
                     /locus_tag="ACHKA1_003699"
     CDS             20205..20468
                     /gene="stxB2b"
                     /locus_tag="ACHKA1_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO5681294.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
     gene            20990..>21465
                     /locus_tag="ACHKA1_003700"
     CDS             20990..>21465
                     /locus_tag="ACHKA1_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO5681295.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISTTSEPEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYG"
BASE COUNT         5134 a         5117 c         5447 g         5767 t
ORIGIN      
        1 ataaaataat taatactcta cttcaggagc agaatattaa attttatccg cgtggtgcat
       61 cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgttgcggat ctttcacaat
      121 agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc ctacgcattc
      181 ggtacactta tcgctgttaa tctcatagat atgatctccc attgaaatcg cctcattcgg
      241 gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta aagacatttc
      301 aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg tatgctttac
      361 tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact cagtccagca
      421 acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg tgtgagcttg
      481 ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac ataatccgga
      541 tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct gtaaaccccc
      601 ctataccagc gcacacatga aaatctgtgt tgctgccgct aaaatcatcc tcgctgcttc
      661 cggcgtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg atttcagcaa
      721 agccggtaag gttacatttt atgccgagtt tccaaaaaag atgggcctca aaggtaaaaa
      781 gctcggcgag tggccggagc tcgctatcca gctggcgcgc gaaaaagcgc taggtatggc
      841 tgacggtggc ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc gggatgacct
      901 caaagccaaa gtcgcccggc agaagctgag cccggacagt ttcacaacct acggggtgcg
      961 tatcgaccgg attaaagcca cgttcggcga gcgcgaggtg ttcagcgacg taacatacaa
     1021 tcggctggtg gaagtgctgg acgagtggat cgccactcgc tcgaacaata acgccctgga
     1081 gttgtttgcc gagctccgtc ggttctggaa gttctgcgca cctactcttt gcaatggccg
     1141 caatgttgcc gccagtctgc cagatgatta tgtttcctcc cgcgtacaga aacccacccc
     1201 cacacggctt tttaccgata ttgaatcaat cgcccgactc tggctcaatg ttgctgcctg
     1261 cacctctgta caccagaaga atgctgttcg cttcatgatc atcactggtg ttcgtccgat
     1321 taatgtccat aacctgcgct gggactacgt tcacgaggag gctggtgaaa ttgtttatcc
     1381 ggaaggggtt atcggcatgc gaggggctat gaaaacacaa aaggctttcc gcctgccgat
     1441 aacgcctgag atccggcgga ttatcgacga gcagaaagcc tggcgtgatt cagttcctga
     1501 gtgcaacagg gattatgtat ttttgcagcc acgtgatcca atgcagccat tttcaaaacg
     1561 atcactggat aagctggtga aaacatacag cccggacggg gctgtaaaag gaataaaaca
     1621 tgatgggact gttaaaggga aagacggtgc atttaatacg atgtgccgta aattccttaa
     1681 gagcaatgtt attgccttga tgaaggaaag aggctattcc cgctcagacc gaagggaaat
     1741 cagcctcctt tgccttcacc actccagcaa gtcagatgac ccgatggcag aacattacga
     1801 cttttctgat gagattttac aggaagagat tgcgttgaag cgcgaagctt tcgaggctca
     1861 cgagcggagc atacttgcgc aggtggcatt gctacggcgg cgaggttaat actggctgcg
     1921 acatttttga ataaaagcgt cgacatttcg gcgctcataa cgaactactt ttgcactgaa
     1981 acgaattggt gccaggatag cccgatgacg atgcttaata ttccactcac atagcgtttt
     2041 ctgtgtaata cctaactttt ggcatacttc atccggggtg agtaaatcgt cgggtttctc
     2101 gctcatgcta tacctctctt tttcatggca tcgagcagga tgtcctgcac tgttcgtttt
     2161 gagttgcgcc gttccatcac catttcgtcc atagtgtcgg cggcgataat gtggtgaata
     2221 aataccggac gattgtgtcc ggcctgtatc tgcctggtgg ggccgatacg ttcaataatt
     2281 tgctgatatt gctccaggtc ccaccagtgt gagaaaaata ccagtatatt tccgccgtcc
     2341 tgcatgttca ggccgtggcc cgcgctggct gggtgtgcaa agagaacagg aatctttccg
     2401 gaattccagt cgcgcagtgt ctgtggatcc tggtcgaggt gacgaccgcg agggaatgct
     2461 ttaagcaagc gttcaagatc gtgtttccag tgataagcaa ccagcacagg tgcgccagct
     2521 gcttcggtca gtatgctgtc cagcgcctgt agtttggtgt catgcagttc tgaccaactt
     2581 ccggtgtcgt ctgtgtatac tgcgccactg gcgatttgaa gacacttcag tgtctttgcc
     2641 gcggcgttcg gtgcttcgat gccttcgcca ttcagctcga ggaacatttc cttttccatt
     2701 tcacgatact gctgacgggc cttcggcggc atatccacgc ggattacgtt atggatgggg
     2761 tctttgatat cgaaccagtc ggccgcatcc agcgagaggg tcacatcggc taacgctcgc
     2821 tgtatttcag cctgtgagtg agcaaacggc tccagtttgg tccagctctg ccccggaaac
     2881 tgtatcgagt tgaaccagcg tgaggtaaac gcgccgtaag tgcgcccgag acgttgcccc
     2941 tggtccacaa accacaattg tccccacaaa tctaccaggc cgttcggtgc tggcgtaccg
     3001 gtgagattta tccagcgctg gacatactta tgcgccactt ttcccagcgc cgccgcgcgc
     3061 ttaccaccac ctcgcagccg gaaggatttt agccgggtgc tttcatctgg aatgacagta
     3121 ccgaacggcc atcgttctcc caattcctca accagccaga caaggttatc gtagttgatg
     3181 gtgaacacgc tcgcgttgct gttcgccagc gctgcagagc gcgctttggc gttaccaaca
     3241 atcggctgca cctcgatatt gcgcagatgc ccccatttca gcgcttcatc cggccatgtg
     3301 cttgctgcaa cgcgtagcgg cgcgaggacc agcgcgggct gtgtctctga gcccgccatg
     3361 aaaagatctt ccagcgtggt gagtgtcgct acggttttac ccatccccat ccctgcccag
     3421 atgttgcagc gggaaatacc tatttcgtgg ttgataatta gatcttggta ggggcgaggc
     3481 gtgaaaattt gtcccatagt attttagcca gtagggcgcg gggtaatccc gcgcaataat
     3541 gtcagattag atcggtgtag taggtgaagt tgccaaactg cggatgtgtc cagcgcttgc
     3601 ggttaccttt cggcggtgtc gattcgttta acagcttttg agctgcttgt tcgatcgccg
     3661 gaaggttaac gagcaatccc cgaacaccgg tgtctgaacg taccggtatc ctggcgaagc
     3721 cgatcaattt ccggcatgtt gaatcagata aacctgtttt ccatgctgct ttgctgacgg
     3781 ggacatattc accttcaaca tgagataaat tcgctggtgc ggccgattct gcttgatatg
     3841 tcggaagggc aatcacctcc gacatcgctt tcatggttgc tgatgcggtt gcctccgcga
     3901 tgacgcgagc aagctggaca attctactat ccacctgaat tacaggtgcg gggtgagtga
     3961 tagctaactg ggggggctcg acttcttttt tcagtttttg ccagtgctta atcatcgcca
     4021 tacgacgaac ggcatcgtaa cctgttacaa ggcattcgga gtgttcatag tcaagttggt
     4081 actcccatgc gtttggatta tcggtattgt actgcaggca attaacacac tgattcgtaa
     4141 ggtaaagcaa atcggcttta cggatataat cgcgctcatc accgccgtaa agctttgcca
     4201 gcattgcacg aatatcacgg caaacatcgg catggttttt atttaccact tctgctatct
     4261 cgcgactgct catcatcggt tgcttaccag tgcctgctag tgttaaagtt ctttcggaca
     4321 ttgaagtgtt ctccattcag taaaagatgc gctgatagtt gcagctacca gcgcgttttg
     4381 tttattcatc ccacggcttg tttgcagcct cccttgcggc ctgctgtgca aaataaatca
     4441 attcaaaagc taactgctct cctttttcca gcgaactgag aagtcttgct gtttccagca
     4501 tgtccgcgat atacccgaaa atatctgcct gatgtaactg cgtgatctga atagccatag
     4561 cgttaacctt gttttgtact cattgtgagg actttattag tactcacaat gaggcttgaa
     4621 gtcaacatgt ttgtgaggca tgttgttctc acactttcaa gggatcatat atgagcaaaa
     4681 gagacgaccc gcagttgagg gtacgcatac cagaattgct aaaagaggct ttggagaaga
     4741 aagcacgggc taataagaga acactgacct ctgagatcgt tagccggttg gaatctacta
     4801 ttcttcagga caaattactt catacatcta gggggtttga ggaagctgct gatgagattc
     4861 tcatactcag ggatcttctc gagaaactta agacaacgta ccatcgagag tatcaggtcg
     4921 aatgggttta tgaaaacaaa tacgagctta ttgaagtgat ggaaaaactt aagaagttgc
     4981 taaatcctga gaatgaatag tggattcatc ctctaaactt ttacgtaacc gccgggtgat
     5041 ttcggcggtt agtgttctgt cgttttctct ggaaattttt tccagtgtgt ctttcaagtc
     5101 ttgcgggatg cgtacccgca attgtgggtc atcacgttta ctcacaatat tccctccaga
     5161 tttttgctat ccagcaccac cacggtaaag cccagctttc gcagacgttc atgctcgcgc
     5221 aactggtcag gccgtggtgg cttgcctggc gctttgcatt cgacaaagac gatgcgaccg
     5281 ccgggtagca ggacaatgcg atccggtacc gagcggcgac cgggtgacac gaacttaaag
     5341 gcgaccccgc cagccttttt cacttcggcg acgaggtgct tttcgataag gctttcacgt
     5401 tcgtaggcca tttacttcac ctgcttttca gccctcagta ccatctggct accatcatca
     5461 agaccccagc taatttcgcc gccttctgcc attaccagtt gccaaactaa ctgcgcggct
     5521 tcgttcgtta cgtcacgccc agggtcattg ccaactcgca agcgaccgcc gtcaacatcg
     5581 cgcatttttg ccaacatgat ctttttggat agcggggaga accccagttg tagtttcgct
     5641 gtattacgca tcattcttcg accgccttac gcttttcgcg catgttctgc atcagacaaa
     5701 aatcagaccg gcgttcgctc cagtcctgat tcagttcgtt acgtgactgg cggttagcct
     5761 tggcccagac cttcgctgcc cggtcatatt cgccggactg ctcaaggcgc aaggcctccc
     5821 tcgcagccct gtaataaagt ggattgtccc gatatttaaa tgacataggg gttactcctg
     5881 ctttgcttcg attgggcgga tgctgctcag caccagacga gtgcggacac cgacagcacc
     5941 accgcggcgg ccagtgtctt tgtagtaata ctcttttggg ccagccaccc acgttgtcgc
     6001 gttttgatgc aattgcacct gcttttcacc ggtgcgggta ataaccgttc cggtatgcgt
     6061 tttgattaca gtcatagcga ttaatcctta cggtagtggt acgcctcaaa accgccagcg
     6121 ttcagtggga tatcgggcgc ccattcgggg ttattggaga gaagcgcgga aagctcttta
     6181 tcgttgaaat cttctgtgtc aggtgattcg gtgatcacct cgtcgtgtac cgtcagcaca
     6241 atgctgtaac cggcatcttc gataagcggc atgtttccgg ccagaacgtc gcgggcggcc
     6301 gcctgggtga cgttctccac cagctttccg ccgtaggttt tgagtcgttg ccatttacgc
     6361 gaataagagt taacacccat gtaggtgata ttcccttgtt cgataaccgg agacgggtag
     6421 catacagcgc gtccggatgg tagctgtatg cgcagccacg cgccatcgcg gcggatttta
     6481 agataaccgc aatacaatgt tttttgcggt gtggcgattg cagtgcggac ggttcgctcc
     6541 agttcgtacc agaaatcgca ggtcgccggg tgcgccctgc gccacaggcg cttgagcgag
     6601 tcacatgcga tgaatacacg ctcggacagg ccaaaggtcg acttacgttt aaccgattcg
     6661 ttgtaccagc ttttcgcctc gcggataaca tcgcggggaa tgtttggcag tgcggcgttc
     6721 gccagctcgt cgagatcgag accgtaaacc agagcaaaag tgatgaaagc cgatacacca
     6781 cctccataac ccagaccgag ttccatgact ttaccgatct gacgcatgtg tttatcaaca
     6841 tcatctggtg caatatcgaa agcttttgca tacgccagtt tatataagtc cggacccgtt
     6901 ccggcgtcat actctctgaa tgcattcagt ttccattctt ctcccgccag ccatgccagc
     6961 atgcggcctt caatgttcga caagtcactt accaccagtt ttttgcctgt tggcgcgata
     7021 atgcagccac gtaacgctga actggttagt tccatgatat tgtcaaacag caggtctgca
     7081 catccggctt tcagtgcttc gatgccttcg tctatttgtt cctgttttag tgaagggcgg
     7141 ggaaggttct ggggctggaa tagccgtccg gcccaacgac cggtacgtga cgccccgcag
     7201 aactgtagcg taccgcgtaa gcgcccgtcg tggcttacgc ctttcatcag tgccttgtat
     7261 ttactggtgc tggtagtact ggcttgcagg cggatagcca gcagttcttt cacggcagat
     7321 ggtaaatggg ggtcggcaat acgacgttcc agagtactgc gttgcatgtc tggtagctcc
     7381 acaccgtagg attcaacaat gtgcttaatc aacgcgtctc gttgtgtggc tgcctgcact
     7441 tcgccatcag tcatttcctg tgtacgcttt gccaggcgct tttgctcctg gtctaccgct
     7501 tcgatcgcag cgcgcgcgag ttgcatgtcc atgcagacgc cccggtcgtt gatctgctga
     7561 tcacgatgcc agagcgccag ctctgtcccc tgataattcc acttcggcag acgtttatag
     7621 acttcgcgca ttgcctcgat atccagtccg gcgtaagcaa caaagcgccg ccattcttcc
     7681 gggtgggttt tactggtggc ccgacgcagt ttgctgtttt tcgggcgtgg cttacagaac
     7741 agctggatca gcgctttacc ttctttgtcc ttcgctttgt cttgcgggac gcctagtact
     7801 tcgcagagtt cccccagaga ccccgggaga ccgtgcgcca acgcctgcac catcgtgtcg
     7861 cgccaacgtt cgactggcgg tgccagtcgc ggtattgcat aacgcagaac ggtgcggtcg
     7921 aagtgagagt tatggaaata aagcagggtt tcagggtctg cgattgcttc gtataagccg
     7981 tgtggaatac caccaccggc agtgatatcc cacacgttta ctggcccgtc gttgatagcc
     8041 catgcgaaaa gcatcacttc aacgccttcg gcatacgcat gggtgccatt attgataggt
     8101 atttcacaga aagtttccag gtcgccccat agtatattgg acattgtatt tactccgaag
     8161 aattagctac atggatacga aagatattat ctcacttgtt ggagtcataa tttcagccat
     8221 aactgcacca gtcggtgttt ataagttatt taaagatgct agttggtttt taccgaaaac
     8281 aacaaaattc tcgcatattc tgaagaatta tccggagcat attgagcctt ctgatgtaga
     8341 gtttatgaag gcagaaatca aaagagaagt taaaaaaagt atattaggta taagtagcca
     8401 gaaattaaga acgttagtta tttatgtcag aacgtattct gaattgaaaa tgccgttttg
     8461 gcagtggggt tatttagcac cccatattca gtgtaaatac gatagattct ttatacgcta
     8521 caaaggtaaa tacaaacgat gtcgctggtg ttctaaagtt gtttctatct tttatttggc
     8581 agatggcttg atatttttct gttggtcgtt aaattatggc gctgttttta taggaatggg
     8641 cgctgtttta atgttgctgt gtatatggat ggcttttatg ttttggtttc tttttccggg
     8701 acgaggcgtt ataaagaagt ataattccca attattgaaa atagatgcaa gtaagtatca
     8761 ggccaaatga tattacaggt ctgatacttt gcttttttaa atcagtgttt tcagacaaaa
     8821 cgaagtcctc cgtaaattaa acggtgataa aaagcccctg gctgagcagg ggcgaactga
     8881 aggattaacg aggtaaagtg ggtgggatat atctgccgat gcggtcttct atggtcggca
     8941 gaatgcacat gctgtcgctg aagcggtcat gcaactggta aaccagtttc gaatccattt
     9001 tttcgagagc aggcattaac tgcgcccgcc agatttcgtt gatgcgccgg aagttatgac
     9061 agacggcgcg gagattgttg atgaacattt ccttctctgc gtcactgact gctggctgcg
     9121 ctttctgaat gcgtttcgtg ctgccattcc ggtagaagcg ttcctgagct gcaacagcct
     9181 cacccacggt tggatagcac ccgacgtaaa cgcttttctc tccgtcccat gcataagccc
     9241 tgtacggatt gtatttgctc cggtgatggt aaaccatttt cggcagacac tccctgtgcg
     9301 gcgccagtat cgtggaggat tgcggaacag cggtttcttt ctcgataagg tcaagcaccc
     9361 aacggcggaa ttcttttgct accggagttg ttgcgaacat agctaccagg tgggcaccgc
     9421 ggagggagaa aatgcggcgc gaggcctgta aattccccga ggtaaccatt ttgattacct
     9481 cggacatgcc gagagtgaac tcatccgtgt tgcgggtgta aatgttagtt acgctgcgag
     9541 catccgaata ttccagcgcg gtagctaggg tagccgcagt aaaccaagtt ttgccgttat
     9601 gttcgatcgt ttcgagaacg tgggatttga atacgatttg ggtactcatg acgattttcc
     9661 tcttaactga gttaatcgcc accatcaggt gctaatcatc gtggtggcga actgtgcggg
     9721 gttagcacta ccgggttaag aggaaccggc gcggatttct ccgcccccac acagcccgcc
     9781 ataatgcgaa cgtggcaaag cttgcgacaa taaaaagacg ctggcgcgtc tggtgtcgcc
     9841 tcttaaactt ccggggtgct aatcccgatc tccatttggg agattcggtg agcataaacc
     9901 ggaagggggc gatgagtcaa tatcggggta gcagggcgac tgggtgctaa ccagaacaat
     9961 acaggtagct ccgggcatat tcccctttgc aaagacaccc ggcgtttaac cgggcgtctg
    10021 tgaagcagga ttaaatcagt gcttcagcat cagcaccttc gctgatatcg tcgaaatcgt
    10081 cagcgcttgc tactccgccg ccagcgaatg catcgccgtc tcgcaggaac tggactccgc
    10141 cgagtgaggc attaatgcgt ttaccgaaat tattgtcctg tgcccagata tcgataacgg
    10201 cgtttacata gcaccctgca tagggacgtc catcagcctg aattagtggc gaacgatcgc
    10261 gatcaagaac ggccgggcgc gctttgttag cagcattcag gaagaaatta ccggggaagc
    10321 cttcatactc tgctttttca tcaccatcat gcaggcacag attgagtttt ttctccagct
    10381 ggttataaat ggactcccac ttctcccccc atttttcctt cgctacctgc ttcatagctt
    10441 tacggatttc ttccagttgt gggtgtttgg gagatattaa aaatactgcg gagaaacgcg
    10501 gatcgccttc gccgtttaca gttttagctt caaacagagc aggaaaagct aatctcacgt
    10561 tatttaattt aatcttcatg aaagtctcct ttaatcagat gaggtctgcg gcgagcgtat
    10621 cgtcggatac gtcgtcgaaa tcatttacag ggttgatatt gagtgcgggg cgtgggtccg
    10681 actcgggaac gacggttggt ttaccatcag ctcgtgttat cagtgcctcg acttttgacc
    10741 aacggcgcgg actggccttt ttgataagtt tttcggcttt ggttgggcta atcagcttaa
    10801 gatcgaaaac ctcctcggtt ttataacgga actggtcttt cagcagcgcg cgggcggctt
    10861 cttcatcact ccaggcccgg ttaccttgtt ttcctgttac cagtttaaac cccggtaccg
    10921 gatttccggc attgagttca ttatgaaccc ggtcccgtac tgcctttagc caggattcaa
    10981 taaagtcggc ctgactatag atctccgcaa gctgctcaat ggttaacaga ggtacacgtg
    11041 cgctggcatt ggtgatgatt tcgctgacag gctttgtcag atcttcaaaa tcgctggccg
    11101 ctgtttgtaa atgctgcatt ttctgggcag tgcaaatagc ttttgcttta cagaagcggc
    11161 actgtttttc tccaggtatg aagttttcca gcggtagtgt ctcaatgcct tcgcattcag
    11221 caatattgag aacaaggatc gcactggttg cggcctcctg tgctcgttta ccgaaagact
    11281 gaagctcctg tacggttaaa gaccattctg aaacgtggtt gagccttggc tggtgaataa
    11341 ataatcttac agtctcaaag tcatacagca tgctgaattg ttcgagagca cccagagcat
    11401 acagttgtag ttgctcattt tgttctgcat caatgcggac gcctttgcca tatttcaggt
    11461 cgtggatttg taattcgtta ccagcgatga ttatgccgtc ggcagttccg aaagattctt
    11521 ccacacccgt tatatgtgag aaatcaacac gttgttcaac caatagttca ttattctgtg
    11581 caagagtcca gaccgtatca acataccggc caacggcttc gaccatttca tcatccacct
    11641 gtgggccaga tgtatcatca ggattttcgc gaagggggta tgagccgaga aacatagaaa
    11701 cattgcatcc ggcgtagtgt tccgggtggc tttgcctgtt tcgtagaact ttttcagcaa
    11761 gcgcgtgcgc tgcagtgccc tcgattgcaa aagttgtttc tttatccggt tgtgtggcct
    11821 ccagcgccag actacccgca caccttagcc aacgatgtgc agatgatggt gataatcgtg
    11881 catgaacatc aggcattcat gacctcctga atgtgttttc gccgcgcagc cgctacgagt
    11941 tgtttggtgg ggaaatgacc gagagatttg aatttgccgt ttaatcgtcc gtaggcctgc
    12001 cacgggtttt tcttaccttc gctgaaccga acgccaacca ctccagattt attttttctg
    12061 gtgtgagagt tagccctgtt ttgtgaaaga gaggcccagc gaagatttac cgcccggttg
    12121 tcatctcgac gaccgttaat gtggtcaact tcaggggcag atccgggaat aaatgcagcg
    12181 gcaacgagac ggtgtatttt gacggttacc tttttacccg ctttccataa gtcggtgatg
    12241 agatatccgt ctgcgtcata gcgtggtttt agtgacttac ctttaatggg ataggtgcag
    12301 ccagaacctt tgtttaatac agttctgtcg aggcttctca ggttgccagc gtctgaaatc
    12361 tggtaaattc cttcaaagcc tgaaatatcc ttccaggctt ccggcatgat taaccctcca
    12421 gtgctttttc agccagggtg attacttcag cgagattttc atctgttact tcaccaagtt
    12481 tcctggctcc ctgtttttcc agaattgcaa tagcttctgc ccggtaaccc ccttttgcta
    12541 actggaggat caacccttca gcttgtttgc gtagtgccgc gaaatcaatt gtatggtcat
    12601 ctttggcgtc attattctgg ctggaatttg ctgcgtctct gcgtgcaaat tcttcctgca
    12661 gctgaaggta ctcaacacgg ttgatctcga tatggccttt tttcagcatc tcgttcaact
    12721 tgcgtaaggt gtggagttca ctggctgctg tgccggatac atttttgacg taaaacggcc
    12781 ccgtgcgttc tccatctttg ttactggcct ttttcggctt aacttcatca cgcccatctg
    12841 caggtgcatc aagtagctgc tcggcaaaag cacgtcgctc gccgatggtt ggcaggtcgt
    12901 cccagaactt aagaatgtta cgggacaggt ccaggagagc aggtttaagc agcgccctgg
    12961 ctcgtttgac gccctgtaat gcgctgtcga gagcatcaat ctgaactact cgtttatcgc
    13021 cttcagcatc acggtaggca acagcacgtt gcagcatgtc ttctgtgata ggggtggcta
    13081 ccgggtagaa accagccagt gcgataacgt cgctgaactc cagatcatcc agtgtcattg
    13141 ccgctgacat gttttcagct tcagttgctg tatcccgaca ttcctgcact cgtgaaatcg
    13201 tgtcaggatg cataacaata cctgatgcca ttgtgcggat aagacgttca agcagcgcat
    13261 tatgttgttc cagaagttga ttattaagtt cgagactggt ttctaaactc atactgtggt
    13321 cctcgctaca aggagaatga aagtgatgat cagaccgagc gcagtggcaa cggccagacc
    13381 ggtcatcaaa tcgaagtttt tacggcgata acggagaaca tcgcgccccg tcagtcgata
    13441 gaggtgttca ggtttcatcg gttgtgctcc taaaacgccc atgcgctttt tcggctcgga
    13501 tacgtgcggc aatggcgcta tcaagttcgg ggaaataccc gaggtggagt ggtttaccgt
    13561 taacggtgat ttgtgcccgc caacgctttc ttgattcttt ccagttgacc cccattgcgt
    13621 tttgtggggc tacgctgtgc aaggtttttg gcgttctcag cgcgtgtgac cagacgtaag
    13681 ttttcaatgc ggttatccgc ccggttatga ttgatatggt caatttccat acctgcgggg
    13741 atatcgccga agtggtattc ccagacgagg cgatgaacgt actgattttt tccatcaagg
    13801 cagatggtga cataaccgtc tgcttttact tttcccgcgg gcttgcctgc catgcgtgaa
    13861 ttagtggatt tccatgcgtg ggggcttttg aaatgcgatg cgggtcggta tttccatata
    13921 agctggccgt tctggtattc aaaagcctcc ctgattctct ttttcaacgt aggagtcctc
    13981 gtgaaggtag gaatgaaaaa aagcccgtca tgggaggcgg gcaaagacta cacatagcaa
    14041 ttacatggat gattcagaag aattagttag cggacggatt gtgtagccat aaccgagata
    14101 gccgttgcgt tcctggtagg ccgccgctct tagtcgggct gggttttcgc ctttgacggt
    14161 tgcaacgacg taatcagtcg cgtcaggtcc gttttctacc acttcaaaag ccggagcgta
    14221 ttttgttgtg agctcatagg tattcatttc ggtacccctt cagtggatta gtaaaaggcc
    14281 cgaagccttt gattaattca ctgcacgccc catcatcggg gcgtttcaat ttacgtgact
    14341 tatcagctcg tcgcggtgtt gtcctctacg cttaccgtac gcatacggac tcggcgctta
    14401 cctcgatccc atcgggtgcc atttcatttt gccaggagta ctgcggctta cctgtcacgc
    14461 ggttcagttt gttaaagagc gattacttag tgcggttaaa aatacaagca aacctgtaag
    14521 tgcgtcaaca ggtattctgg taataaaatg cattattaac ctaattactt gtttttacag
    14581 gtaataaaaa aagagggcct aagccctctt tgtttagtag tcaggatgcg attatctttt
    14641 tctgcggaat atgcgatgtt ctaccattgt ccctatgatc ttaattggtt tttcccagga
    14701 gcgacaaata gggtaatcac tattcaatgg aacaagttca aagtcttcct cacccttagc
    14761 gccaatccct accgggcggt attttttgaa cgttgcttcg tggctaccgt tacatgccgc
    14821 aacgaattca ccaggagtag ggtatacgtc agtgtcaatt ataacgatgt ccccctcttt
    14881 aaattcaggt tccattgaat ccccacgtat acgtagcgca aatgaacttt ctgatagctc
    14941 agccgtggtg agaatgtatt cgaaatcacc ttcttcacga agtagctcag cgcttgtgaa
    15001 agcccctgct tgtacgtagc ttagaacagg cacccttcgg gctccgtagt tgaaatctgt
    15061 aacaacatcg cccccaagaa gtagccattt aggatcgcat tcaagagctg acgctaaagc
    15121 aagaagattg cgtggtttaa gtgttttccc gttctctata gcttcaatcg actgctggct
    15181 aatgcccgct cgtttcgcaa cttcgacctg agttaagttt agttctgttc tgcgtttttt
    15241 agctcggttg gcaagattca tgttgactcc ttttctgtca ttgatgatta cagatacttc
    15301 tgtatttgac aaacaaggtt gcctgtgagg taattacaag taaacctgta ataatgaggg
    15361 cttactcaat gaccagttcc acccttgctt cacgtataaa ggagcgccgc aaagctttag
    15421 gtataaccca aacttcattg gctgaaagtg ttggcatgcg ccagcagtct gttcaatacc
    15481 ttgagtccgg acgcgccaca cgtaccggtt tcatccttga attggcaaaa gttctgaaat
    15541 gcgatcctga ctggctatta aacggcgaaa attcagagca gcaaaaggtg taacccatgc
    15601 ctgataacaa accctgggga gctacgcccg atgagtggtt ccacttcgat ctggtgttgg
    15661 ggatgacaga caggctttta cctgtggtgt ccaatccctg tgcagaactg tcgcctaata
    15721 gcaagctgtc tcataatttc ggcaaaactc ccagtcgcta caatggccaa cgtaaggttg
    15781 ttggtatccc cgactgggct aactatgttg ttacggaaaa cgattttgcc cggtggtcga
    15841 acgaacccga ttacggtatt tgcgttcgta ctggcgacgg ggtggtggct ctggactgcg
    15901 atatcaatga cgccgggatg caggagatcg tacgcaatat tatcctgtca tgcctggggg
    15961 aacttccgcc acgtcgctgg cgggctgaca gtcataaatg cctgtacctg attgctgttg
    16021 atggcgacta ccgtaaacgt ggtcaccgcc tggaaggcga aaacaaacaa atagagctgc
    16081 tcgctaaggg ccagcagttt gtggcttgtg gtacccatcc ggcaggcgaa cgtattcagt
    16141 gggactgcgg tttgcccggc gaacctctga aaattacatc tgaacagctc gaaagcctgt
    16201 ggcagcgtct ggcggataac ttacccgtga aagacagcta taccgcaggt gcaggacgtc
    16261 agcgtgacct ttcctgtgtt gatccttccg cgacagatga tgtcgctgac tggcttgacg
    16321 cgaacgggtg gactctgtca gtcagctctg atggttcccg caatctgaaa ccgtttcggg
    16381 atgagtcaga atacagcaac ggttgcagcg aaacatccat taagtatttt ccaaaaggga
    16441 cggggggatt tgaactggga cattttaaaa gcatgcacaa caccgatgcg gggcttactg
    16501 atgccgactg gctggatgca tacggctacc gcgtggcgga cttcgaagat ttgacagtgg
    16561 ctgaagatgg cgataagcct gagtatcttc cctggcctaa gttcagtcgt gataagaatg
    16621 accggataga aggtaccgtt accaatgtgc tgctggcgct gcggcgaccg gatctctgcg
    16681 gtgtacaaat ccgccttgat gagttccggg acgagataat actgatcact ccacggggga
    16741 aaaacctgcg gttgcgtgat gagttttata ccatgctgca ctctaggctc gaatgtctgg
    16801 gattcaagaa atttgaagag gcagcgatca ggcgtgcggt gcgcctggtc gcgtacgaaa
    16861 accgttttga ttcgcttaaa gactggattg ccaatcttcc tgcatgggat ggtgttcccc
    16921 gtatagatac tttcttctgt cggcactggg gtatcgaggc caacgcctac acccacgcag
    16981 tcgggcgcta ctggtggact ttgctcgcgg gtcgagctct ggttcctggc attaaagggg
    17041 acatggctgt gatccttgtc agccaacagg gtaagaacaa gtccgaaggt atccgtgcaa
    17101 tggctccaac ccctgagcat tacatggaac tggacttcgg taagaaggga gaggagcgca
    17161 ttcgtgagat gcgagggcat aacgttattg agcttggtga gatgcgcggt atggataaac
    17221 ggggtattgg tgatgttcgt gtcaccatat ccacccgtgc agaccgcaac cgcgggttgt
    17281 ttcgggagca ctatgtcact ttaccgaggc gttgcggttt tattggtact tctaactccg
    17341 atacgccatt gacagatacc gaaggtaacc gccgttggtt gccgatgacg ataccggatg
    17401 agactgacgg caagtttatc gctcagggta tcgctgacga aaaaatacag ctttgggctg
    17461 aagcagtcgc cgtttttaaa cgtgatggca tcgcatggga gaacgctgaa acactggcta
    17521 agtctattct tggggattat gaggtcaaag atgaggtctg gatgtcatgc gtcgctgagt
    17581 ggttagagtt gccacaggat ctgtacgata tggctgccga cggcacgaaa aagaactgcg
    17641 aacgcgtccc ccttacgtca aaagacgtat tggtcgaggc gattggcttc aaagcccccc
    17701 aggtaaaacg cagtgacgaa atgaaaattg cagcaatcat gaagaagctt ggttacaaaa
    17761 ataaacagtt gcaaagttta ccgggtaagc ctcgcagctg ggaaaaatgc aaataactac
    17821 aagtttgtag atttttgcag gttgttactc aacctactca acctcacaag ggtaggttga
    17881 gtaagaaaaa tggtattaaa atcatgatat tactcaacca ctcaacctac tcaactattt
    17941 tatataaata tacgcgtgcg cgcgcacgca tgtatgtaag aattttattt ttccaaaagg
    18001 ttgagtaggt tgagtaagag tatgattttt atgtttttta ggttactcaa ccttttgccc
    18061 gtaaaaaagg ttgagtaaag aaaacttgca gaaaatcaca aataaccaaa cgaagtaaaa
    18121 acatgaatac aacctgtgca agattaccaa gtgcggatac gttagcggaa caggcagcaa
    18181 aaacggcaag tgggtggatg tcagacgccg taatagcgat agatgagctt tttggaaagg
    18241 gctatgcgaa gcaacaccct gaattagttg ctgcctttat gcaaacagcg ggcttagatg
    18301 aagctgcaat gcacttacgg ggacttgtgt tagctaacca acggattgat gacacactgg
    18361 aagtgctttc ggagcatatt atgctgctta acgagcccca ataaacccga tgcggataaa
    18421 cagacgtagt tctcagaaaa ttttttcgta gcaaaacgta gaggccagcg ttatgcgtag
    18481 agatatgcaa cttgttctgg agcggtgggg gcgatgggcg gcgagcgagg aatactgttc
    18541 aatggttgac tggccctcaa tgtcggttac ttcccgggac gtaaccgata gtggcaaacc
    18601 cggttgctct gacgaggacg gagcggtgat tgatacctgc atcgcgcata tgagtatggt
    18661 ttgcccgtac gatgatttgc tgatcctggg gttacgtttt ataggcggcc tctcaacgcg
    18721 tgcaattgct gaggcgatag accgtagtca tcttagcgta cggacatccc tgaaggcgtc
    18781 tgaagcattt ctggaaggag ctctgactct gcagggagta aggttggata tggatcctga
    18841 agttgtacta cctgaaaggg ttgtgtgcgc acaaaaaaat atgttatggt tatagcgtct
    18901 ataattgtat gcttactgct gaaaggttct gataacaccg gaaccttttt tcttgtcttc
    18961 tgctgggaaa aattaataaa acaggaaaga aaaacatgac atcgcactgg ttcagtgtca
    19021 gccagtggca gttaccaaac gaggatgatt acagaaaact tcatgcgttg ttagctcagc
    19081 cggacagagc aattgccttc taagcaatcg gtcactggtt cgaatccagt acaacgcgcc
    19141 atatttattt accaggctcg cttttgcggg ccttttttat atctgcgccg ggtctggtgc
    19201 cgtattactt cagccaaaag gaatacctgt atatgaagtg tatattgtta aaatgggtac
    19261 tgtgcctgtt actgggcttt tcttcggtat cctattcccg ggaatttacg atagactttt
    19321 cgactcaaca aagttatgtc tcttcgttaa atagtatacg gacagaaata tcgactcctc
    19381 ttgagcatat atctcagggg accacatcgg tgtctgttat taaccacacc ccaccgggca
    19441 gttattttgc tgtggatata cgagggcttg atgtctatca ggcgcgtttt gaccatcttc
    19501 gtctgattat tgagcaaaat aatttatatg tggccggatt tgttaatacg gcaacaaata
    19561 ctttctacag attttcagat tttgcacata tatcagtgcc cggtgtgaca actgtttcca
    19621 tgacaacgga cagcagttat accactctgc aacgtgtcgc agcgctggaa cgttccggaa
    19681 tgcaaatcag tcgtcactca ctggtttcat catatctggc gttaatggag tttagtggaa
    19741 atgccatgac cagagatgca tccagagcag ttctgcgttt tgtcactgtc acagcagaag
    19801 ccttacggtt caggcaaata cagagagaat ttcgtctggc actgtctgaa actgctcctg
    19861 tttatacgat gacaccggaa gaagtggacc tcacactgaa ctgggggaga atcagcaatg
    19921 tgcttccgga gtttcgggga gagggtggtg tcagagtggg gcgaatatcc tttaataata
    19981 tatcagcgat actgggcaca gtggcggtta tactgaattg ccatcatcag ggggcgcgtt
    20041 ccgttcgcgc cgtgaatgaa gagatacaac cagaatgtca gataactggc gacaggccag
    20101 ttataaggat aaacaatact ttatgggaaa gtaataccgc agctgctttt ctgaatcgca
    20161 gggctcactc tttaaataca tccggagaat aacaggagtt aaatatgaag aagatatttg
    20221 tagcggcttt atttgctttt gtttctgtta atgcaatggc agctgattgt gcaaaaggta
    20281 aaattgagtt ctctaagtat aatgagaatg atacattcac agtaaaagtg gccgggaaag
    20341 agtactggac taaccgctgg aatctgcaac cgctactgca aagcgcacag ttaacaggaa
    20401 tgacggtaac aatcaaatca aatacctgtg cgtcaggttc aggatttgct gaagtgcagt
    20461 ttaattaata tcagaagcat tgctggtttc gtggtgtgca gcaatgtagt tacagtgtaa
    20521 tcaatgtcac aattcagtca gttgaaggtt gtctgcccga ctgagaattt gttaaaaaaa
    20581 atcctgcatg gtgaatcccc ctgagcggcg gggcatatca gcgtcacagg tgtttctgtt
    20641 ttacctctat cctttctgtg cgggttcagg tgctgatact gaactcaccg ggaggcaccc
    20701 ggcaccatgc atgaacggta catagcgcat acatcagccc ctctccggag gggctttctt
    20761 gtgggcaaaa aaaagcccgc gccgggagac gcgggcggca aggaataaac aacaaaacgt
    20821 gaagtaatat ttcagctggc gaataatacc cgacagtaat cactctgtgc aactgcgcgg
    20881 cctttttcgt attgcgggct gtagtctcct ttctgccatt gtcctgtaac ttccggactt
    20941 cagcccgctc cttatctgac tcacaacatt atcccgaccg ggaggattca tgacatttaa
    21001 acactacgat gtggtcaggg cggcgtcgcc gtcagacctt gcggagcgac tgactcaaaa
    21061 actgaaggag gggtggcagc catttggcag tcctgtcgcc atcacgcctt ataccctgat
    21121 gcaggccatt gcggcggaag gtgatgtcac cacacctgtg ctggtgaagc cgtcggatgg
    21181 agaaggcaca gttatcagca ccaccagcga accggagtat tactttgttg ttgttctggc
    21241 ggggcagtca aacagcatgg catatggtga aggccttccg ctgccggaga catatgaccg
    21301 tccggacccg cgtattaagc agctggcgcg ccgcagtacg gtgacaccgg gcggtgccgc
    21361 ctgtaagtat aacgacatca ttccggcgga ccattgtctg catgatgtgc aggacatgag
    21421 ccggctgaat catccgaaag cggacctgtc aaaggggcag tacgg
//
LOCUS       DAZFKF010000084        21454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFKF010000084 DAZFKF010000000
VERSION     DAZFKF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.084"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            415..696
                     /gene="drpB"
                     /locus_tag="ACHKA1_003701"
     CDS             415..696
                     /gene="drpB"
                     /locus_tag="ACHKA1_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO5681296.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
     gene            736..1431
                     /gene="yedJ"
                     /locus_tag="ACHKA1_003702"
     CDS             736..1431
                     /gene="yedJ"
                     /locus_tag="ACHKA1_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO5681297.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALDVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPHTMQTIRGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            1498..2916
                     /gene="dcm"
                     /locus_tag="ACHKA1_003703"
     CDS             1498..2916
                     /gene="dcm"
                     /locus_tag="ACHKA1_003703"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO5681298.1"
                     /translation="MQENILVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2897..3367
                     /gene="vsr"
                     /locus_tag="ACHKA1_003704"
     CDS             2897..3367
                     /gene="vsr"
                     /locus_tag="ACHKA1_003704"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO5681299.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDS
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3356..4276)
                     /gene="yedA"
                     /locus_tag="ACHKA1_003705"
     CDS             complement(3356..4276)
                     /gene="yedA"
                     /locus_tag="ACHKA1_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO5681300.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4450..5367
                     /gene="yedI"
                     /locus_tag="ACHKA1_003706"
     CDS             4450..5367
                     /gene="yedI"
                     /locus_tag="ACHKA1_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO5681301.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            5446..5628
                     /gene="yodC"
                     /locus_tag="ACHKA1_003707"
     CDS             5446..5628
                     /gene="yodC"
                     /locus_tag="ACHKA1_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO5681302.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSNGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPVEEV"
     gene            5871..7565
                     /gene="dgcQ"
                     /locus_tag="ACHKA1_003708"
     CDS             5871..7565
                     /gene="dgcQ"
                     /locus_tag="ACHKA1_003708"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO5681303.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWKIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELAMLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(7562..8377)
                     /gene="yedP"
                     /locus_tag="ACHKA1_003709"
     CDS             complement(7562..8377)
                     /gene="yedP"
                     /locus_tag="ACHKA1_003709"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO5681304.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLTRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8675..8902)
                     /gene="yodD"
                     /locus_tag="ACHKA1_003710"
     CDS             complement(8675..8902)
                     /gene="yodD"
                     /locus_tag="ACHKA1_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO5681305.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQHDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            9065..9253
                     /gene="dsrB"
                     /locus_tag="ACHKA1_003711"
     CDS             9065..9253
                     /gene="dsrB"
                     /locus_tag="ACHKA1_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO5681306.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9297..9920)
                     /gene="rcsA"
                     /locus_tag="ACHKA1_003712"
     CDS             complement(9297..9920)
                     /gene="rcsA"
                     /locus_tag="ACHKA1_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO5681307.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10210..10995)
                     /gene="fliR"
                     /locus_tag="ACHKA1_003713"
     CDS             complement(10210..10995)
                     /gene="fliR"
                     /locus_tag="ACHKA1_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO5681308.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11003..11272)
                     /gene="fliQ"
                     /locus_tag="ACHKA1_003714"
     CDS             complement(11003..11272)
                     /gene="fliQ"
                     /locus_tag="ACHKA1_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO5681309.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(11282..12019)
                     /gene="fliP"
                     /locus_tag="ACHKA1_003715"
     CDS             complement(11282..12019)
                     /gene="fliP"
                     /locus_tag="ACHKA1_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO5681310.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12019..12384)
                     /gene="fliO"
                     /locus_tag="ACHKA1_003716"
     CDS             complement(12019..12384)
                     /gene="fliO"
                     /locus_tag="ACHKA1_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO5681311.1"
                     /translation="MNNHATVQSSAPISAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(12387..12800)
                     /gene="fliN"
                     /locus_tag="ACHKA1_003717"
     CDS             complement(12387..12800)
                     /gene="fliN"
                     /locus_tag="ACHKA1_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO5681312.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADTVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(12797..13801)
                     /gene="fliM"
                     /locus_tag="ACHKA1_003718"
     CDS             complement(12797..13801)
                     /gene="fliM"
                     /locus_tag="ACHKA1_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO5681313.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(13806..14270)
                     /gene="fliL"
                     /locus_tag="ACHKA1_003719"
     CDS             complement(13806..14270)
                     /gene="fliL"
                     /locus_tag="ACHKA1_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO5681314.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(14375..15502)
                     /gene="fliK"
                     /locus_tag="ACHKA1_003720"
     CDS             complement(14375..15502)
                     /gene="fliK"
                     /locus_tag="ACHKA1_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO5681315.1"
                     /translation="MIRIAPLITADVDNTTLSGGKASDAAQDFLALLSEALTGEATTD
                     KATPQLLVATDKPTTKGEPLVSDIVSDAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSTQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLIAPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(15499..15942)
                     /gene="fliJ"
                     /locus_tag="ACHKA1_003721"
     CDS             complement(15499..15942)
                     /gene="fliJ"
                     /locus_tag="ACHKA1_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO5681316.1"
                     /translation="MTEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAATRKPE"
     gene            complement(15961..17334)
                     /gene="fliI"
                     /locus_tag="ACHKA1_003722"
     CDS             complement(15961..17334)
                     /gene="fliI"
                     /locus_tag="ACHKA1_003722"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO5681317.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISPE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(17334..18020)
                     /gene="fliH"
                     /locus_tag="ACHKA1_003723"
     CDS             complement(17334..18020)
                     /gene="fliH"
                     /locus_tag="ACHKA1_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO5681318.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPIVEPEETIIEETEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18013..19008)
                     /gene="fliG"
                     /locus_tag="ACHKA1_003724"
     CDS             complement(18013..19008)
                     /gene="fliG"
                     /locus_tag="ACHKA1_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO5681319.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(19001..20659)
                     /gene="fliF"
                     /locus_tag="ACHKA1_003725"
     CDS             complement(19001..20659)
                     /gene="fliF"
                     /locus_tag="ACHKA1_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO5681320.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSEESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            20874..21188
                     /gene="fliE"
                     /locus_tag="ACHKA1_003726"
     CDS             20874..21188
                     /gene="fliE"
                     /locus_tag="ACHKA1_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO5681321.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
BASE COUNT         5094 a         5495 c         5671 g         5194 t
ORIGIN      
        1 agcggaaaga tagggggtat caatgatggt tgcgccaaac acctggtgtg atcacgcgca
       61 cattaatgaa ggcataaaaa aaagccggca tcatgccggc ccaaaaaata taaacaagac
      121 gataaaaggc taataacgga ggcatcatga cacagaataa taaaaaggta taattgtatt
      181 cagtgctaat atattgatta aatgaattaa taaagccgat atcaaagata aataaaatcg
      241 ctcaaaacga tttttcctgt attcactgcc gttgcgcaaa aattatctat ttgttcaaaa
      301 aatgagtgag tcttgactgg ctcatccaat gtggaaaaat gtgactttta tcacataata
      361 gtactaagtc tgaattttcc gggttatctc aaaatggaat acggttcgac aaagatggaa
      421 gagagactct ctcgcagccc tggcggcaaa ctggctttgt gggctttcta tacatggtgt
      481 ggctatttcg tctgggcaat ggcgcgttac atctgggtga tgagccggat ccccgatgcc
      541 cccgtgtcgg gatttgaaag cgatttaggc tctaccgcgg gaaagtggtt aggggcactg
      601 gtcggatttt tatttatggc tctggtcggg gcattgttag gcagcattgc ctggtacacc
      661 cgacctcgtc ccgcacgtag cagacgctat gaataaaaat tgctaaggtg gcgactcttt
      721 ggcaacggag cgcgtatgga cttacaacac tggcaggcac agttcgaaaa ctggttgaaa
      781 aaccatcatc agcaccagga cgcggcccat gacttgtgtc attttcgccg cgtctgggct
      841 acggcacaga agctggcggc agatgacgat gtcgatatgt tggtgatttt aaccgcctgt
      901 tattttcacg atattgtgag cctggcgaaa aatcatccgc aacggcagcg ttcttcaatt
      961 ctggcggcag aagagacgcg tcgcctgctc cgtgaagagt tcgagcaatt tccggcggag
     1021 aaaatcgagg ccgtttgtca tgccattgcc gctcacagtt tcagcgcgca aatcgccccc
     1081 ttaacaacgg aagctaaaat cgtccaggat gcggatcggc tggaagccct gggggcgatt
     1141 ggcctggcgc gcgtgtttgc cgtttcagga gcattggacg tggcgctgtt tgatggtgaa
     1201 gatccgttcg cacagcatcg cccgcttgat gataaacgtt acgcgctgga tcatttccag
     1261 actaagttgc tgaagctacc gcataccatg caaaccataa gaggcaagca gctggcgcag
     1321 cacaacgcgc aatttttagt cgagtttatg gcgaagctca gtgccgaact ggcgggggag
     1381 aatgaaggtg tcgatcacaa ggtgatagat gcgttttcac ctgcgggctg agcgcgtggc
     1441 cctaaatggc tgtaattatg ttaacctgtc ggccatctca gatggccggt taaatctatg
     1501 caggaaaata tattagtaac cgattcatac agcaccggga atgccgcaca ggcaatgctg
     1561 gagaaactgc tgcaaattta tgatgttaaa acgctggtgg cgcagcttaa tggtgtgggt
     1621 gagaatcact ggagcgcggc aattttaaaa cgtgcgctgg cgaatgactc ggcatggcac
     1681 cgtttaagtg agaaagagtt cgcccatctg caaacgttgt tacccaaacc accggcacat
     1741 catccgcatt atgcgtttcg ctttatcgat ctatttgccg gaattggcgg tatccgtcgc
     1801 ggttttgaat cgattggcgg acaatgcgtg tttaccagcg aatggaacaa acatgcggta
     1861 cgcacttata aagccaacca ttattgcgat ccggcgacgc atcattttaa tgaagatatc
     1921 cgcgacatca ccctcagcca taaagaaggc gtgagtgatg aggcggcggc ggaacatatt
     1981 cgtcaacaca ttcctgaaca cgatgtttta ctggccggtt tcccttgtca gccattttcg
     2041 ctggctggcg tatcgaaaaa gaactcgctc gggcgggcgc acggttttgc ctgcgatact
     2101 cagggcacgc tgttctttga tgtggtgcgc attatcgacg cgcgtcgtcc ggcgatgttt
     2161 gtgctcgaaa acgtcaaaaa cctgaaaagc cacgaccagg gtaaaacgtt ccgcatcatc
     2221 atgcagacgc tggacgaact gggctatgac gtggctgatg cagaagataa cgggccggat
     2281 gatccgaaaa tcatcgatgg taaacatttt ctgccgcagc accgtgaacg catcgtgctg
     2341 gtgggttttc gtcgcgatct taatctgaaa gccgatttta ctctgcgtga tatcagcgaa
     2401 tgtttccctg cacagcgagt gacgctggcg cagctgctgg acccgatggt cgaggcgaaa
     2461 tatatcctga cgccggtgct gtggaagtac ctctatcgtt atgcgaaaaa acatcaggcg
     2521 cgcggtaacg gcttcggtta tggaatggtt tatccgaaca atccgcaaag cgtcacgcgt
     2581 acgctgtctg cgcgttatta caaagatggc gcggaaattt taatcgatcg cggctgggat
     2641 atggccacgg gtgagaaaga ctttgacgat ccgctgaatc agcaacatcg tccacgtcgg
     2701 ttaacgcctc gggaatgcgc gcgcttaatg ggttttgaag cgccgggaga agcgaaattc
     2761 cgcattccgg tttcggacac tcaggcctat cgccagttcg gtaactcggt ggtcgtgccg
     2821 gtctttgccg cggtggcaaa actgcttgag ccaaaaatca aacaggcggt ggcgttgcgt
     2881 cagcaagagg cacaacatgg ccgacgttca cgataaggcc actcgcagca aaaatatgcg
     2941 cgcgattgcc acgcgtgata cggcgattga gaagcgcctc gccagtctgt taaccgggca
     3001 gggcctggcg tttcgcgttc aggactccag tctgcccgga cgcccggatt ttgtcgttga
     3061 tgaatatcgc tgcgtgatat ttacccatgg ctgcttctgg catcatcatc actgctatct
     3121 gtttaaagtg cctgcgactc gaaccgagtt ctggctggag aagataggta aaaatgttga
     3181 gcgcgatcgc cgcgatatca gtcgcttgca ggaactcggc tggcgcgtat tgattgtctg
     3241 ggagtgcgcg ttacgtgggc gcgagaagct gacggatgaa gcgcttagcg agcgtctgga
     3301 agagtggatc tgcggcgaag gtgccagcgc gcagatcgac acgcagggga tacatttact
     3361 cgcttgatgc gtcctgaata actggcgcaa ctacgggttt tgccggaaag agatattttc
     3421 ccaacgtgac cagtaccacc gcgaagacaa ttacgccgag cgccagccat tcaatcttcg
     3481 acagtgtttc tccacccagt cccgtaccca gcaagaccgc gaccaccggg ttaacgtagg
     3541 cgtagctggt ggcgagagcc ggactgacgt tgcggattaa atacatataa gcgttaatgg
     3601 cgataatcga accaaacagc gccagatagc cgaccgcaag gaagcctgaa agggaaggga
     3661 gcgccgtcag tttttcaccc gcaatcatcg acgcgatcat taacaccacg cctgccgcca
     3721 gcatctcaat cgcacccgcc atcatcccta caggtaaggt aatgcgcgag ccataaactg
     3781 agccaaacgc ccagctaatc gagccgatta aaatcagaat cgcgccccac ggattgccgc
     3841 ttaaatttcc accgctattg agcatgatga ttccggcaag cccaatggca atacccaccc
     3901 attccagttt gcgcgtttta atgccaaaca ggcggctgaa gcacagggta aagaggggca
     3961 cggttgcaac cactacggcg gcgatgccgg aaggaacatt ttgatgttcg gcaaccgtca
     4021 ccatgccatt accgacagcc agcaataaca ggccaatcag cgcggcattg agcagcggac
     4081 gtagcggggg gagcttgtgt ccgcgcagta gcaaaaatgc cagcaataaa ataccggctg
     4141 ccaggaatcg aacgcccgcc atcattaatg gaggccagct ttccacgcca atccgaatga
     4201 caaaataggt tgagccccaa atgatataca acgcaaacag cgcgccaaaa agcggtaata
     4261 actggcggaa acgcataatc cctcacggtg gaaataaaaa ggtggttcat agtaaacgtg
     4321 aaaatcattc tgctggcgag agatataatt gcacttgatt gttaaaaaaa atgttgacct
     4381 gtgaagcaag tatcagagct gccgtttttg cttcatactt acacccttca acaataaaaa
     4441 tgagagggaa tgcttttggc cgggagtagt ttactgacgt tgctcgatga tatcgccaca
     4501 ctgctggacg atatctccgt gatgggcaaa ctggcggcga agaaaaccgc cggtgtatta
     4561 ggggatgact tatcgctcaa tgcgcaacaa gtttcaggcg tgcgggccaa ccgggaactt
     4621 cccgtggtct ggggcgtggc gaaaggatcg ctgatcaata aagtgattct ggtgccactg
     4681 gcgctgatca tcagtgcgtt tatcccgtgg gcgattacgc ctctgttgat gattggtggc
     4741 gcgtttctct gctttgaagg ggtagagaaa gtgctgcata tgctggaggc gcgtaaacat
     4801 aaagaagatc cggcgcagag tcagcagcgt ctggagaagc tggcggcgca ggatccgctg
     4861 aagtttgaaa aggacaaaat aaaaggggcg attcgtaccg attttatatt gtctgccgaa
     4921 atcgtcgcca tcacgctggg gattgtggcc gaagcgccgt tgcttaatca ggtgctggtg
     4981 ctttcaggca tcgcgctggt agtgaccgtg ggcgtctacg gtctggtagg ggttatcgtt
     5041 aagattgatg acctggggta ttggctggcg gaaaaatcca gcgcgctgat gcaggcatta
     5101 ggtaaaggat tattgattat cgcgccctgg ctgatgaaag cgttatcgat tgtcggcacg
     5161 ctggcgatgt tcctcgtcgg cggcgggatt gtggtacatg gtattgcgcc gctgcatcac
     5221 gccattgaac atttcgccgg gcagcaaagt gcagtggtgg cgatgatatt accgactgtt
     5281 ttaaatctga ttcttggatt tatcatcggc ggcatcgtgg tgctgggagt gaaagccgta
     5341 gcgaaaatgc gcggtcaggc acattaatac tcgcacaggc tattatgcaa aattcgccat
     5401 cttcaactaa ggtgaacaag tttcactcga aaaaaggagg cgagtatgag ctttatggtt
     5461 agtgaggaag ttacggttaa agagggcggc ccgcggatga ttgtcactgg atactccaac
     5521 ggtatggttg agtgtcgttg gtatgacggt tacggggtca agcgggaagc ttttcatgaa
     5581 accgagcttg ttccggggga ggggagtcgt cctgtggaag aagtttgaaa gggaatcatt
     5641 aacaccaggc cggatgtggc gctcacgtcg catccggtcg ggtgtgccag tgtctgcact
     5701 gaattgcctg atgcgacgct atcgcgtctt atcatgccta cgttataaaa caaaactatc
     5761 gatgtctgtc gcgtcttatc atgccaccat tataaaatta tcgctgtttt gcggtacgct
     5821 atcgccaggt tatgccagaa tcataaaaaa gcaggttggg agtcgtcagg gtgcagcacg
     5881 agacaaaaat ggaaaaccag agctggttga aaaaactcgc acgccgcctg gggcctggtc
     5941 atgtcgttaa tctctgcttt atcgtggtat tgcttttttc caccttgctc acctggcgtg
     6001 aagtggtagt gctggaagat gcctatatct ccagccagcg taatcatctg gaaaacgtag
     6061 ccaacgcgct cgataagcat ttgcagtata acgtcgacaa actgatcttt ttgcgtaatg
     6121 gcatgcgcga agctctcgta gcgccactgg atttcacctc tctgcgtgat gctgtaaccg
     6181 agttcgaaca gcatcgcgac gagcacgcct ggaaaatcga actcaaccga cggcgcaccc
     6241 tgccagtcaa tggcgtgtcg gatgcattag tcagcgaggg gaatctcctg tctcgcgaaa
     6301 atgaaagcct cgacaatgaa attaccgctg cactggaagt tggttacttg ctgcgactag
     6361 cgcacaactc ctcgtcgatg gttgaacagg cgatgtatgt ctcgcgtgcc ggattttacg
     6421 tttcgacgca gccgaccttg tttacgcgca atgtcccgac gcgttattac ggctatgtca
     6481 cccaaccctg gtttatcggc cattcacaac gagaaaatcg tcaccgcgcg gtacgctggt
     6541 tcacttcgca accggaacac gccagcaata ctgaaccgca ggttaccgtc agtgttccgg
     6601 tagacagtaa taactactgg tatggcgtgc tggggatgag tattcccgtg cgtactatgc
     6661 agcaattttt aagaaacgcc atcgataaaa acctcgatgg tgagtatcag ctctatgaca
     6721 gtaagctgag atttttgacc tcttccaatc ctgaccatcc aacagggaat atttttgatc
     6781 ctcgtgaact ggccatgctg gcgcaggcaa tggaacatga cacgcggggc ggcattcgta
     6841 tggacagtcg ctatgttagc tgggaacgtc tggaccattt cgacggtgtg ctggtgcgcg
     6901 tccatacgtt aagcgaaggc gtgcgcggcg atttcggcag tatcagcatt gcattaaccc
     6961 tgctgtgggc gctctttacc accatgttac tcatctcctg gtatgtgatt cgccggatgg
     7021 tcagcaacat gtatgttctg caaagctcgt tgcagtggca ggcgtggcac gacaccttaa
     7081 cgcgtttata taaccgtggc gcactgttcg aaaaagcccg tccgctcgcg aaattgtgtc
     7141 agacgcacca acatcctttt tctgtcattc aggtcgatct tgaccatttt aaagcgatta
     7201 atgaccgctt tggtcatcag gcgggcgacc gtgttctttc tcatgctgcc ggattaatta
     7261 gcagttcctt gcgtgcgcag gacgttgccg ggcgggtcgg tggtgaggag ttttgtgtga
     7321 ttctgccagg cgcgagtctg acggaggctg cggaagtcgc agaacgtatt cgcctgaagt
     7381 taaatgaaaa agagatgttg attgctaaga gtacgacgat acgcatcagt gcctcgttgg
     7441 gggtaagtag cagcgaggaa accggtgatt atgattttga acaactccag tcactggccg
     7501 accgtcggct ttatctcgct aaacaggctg ggcgtaatcg ggtattcgcg agcgataacg
     7561 cttaacgggc ggagaaaaaa tggtccagcc cttcacgcca tccttccggt ccttcacgct
     7621 gcgttcgcca gacgcgggcc ggatcctcat catgcagatg caccccttca cggtttagcc
     7681 ctttcacaat caccgcgtaa tccattacct ccagtaaggg cgcatcgttt ggcccatcgc
     7741 ccaggccaag tgtggttggg cgtttgcctg acaattgttg ataggtcgcg ataatccagt
     7801 tggcagcctg atcttttccg gcagaggcat ccagtacgtg ccagaagcgc gcaccttgca
     7861 taaactgtaa gcccagttcg ttcagacgag cggtaaattg tgccatacgc tcgtcactgt
     7921 cgcgccaaat tagcgttacc gacgcctcat gaagctgcgt cagcgccgcc tggctacggc
     7981 ttaatcccgt ccattcggcg atggttgcat cgtcgacatc atcaaaagtc gtgaatttaa
     8041 aatgttcttt ctcgcgtagc gtatttaaaa cctggctgat ttcgccatgg ctaatacctg
     8101 agatgatgcg tggaaaaccg tctatctcct gccattgctc agcaagctgg atcactgcgc
     8161 cattttcggc aatcagcggt aaaccttgta gccccaacgt tttttgcaag tacagcattt
     8221 ccgctgatgt tttactgcta cagagaatga cgggaacatt tgcttcgcgt aaacgggtga
     8281 gccagggggc tgccggttgc cagtcataac tatgactgtc cagcagggtg ccatcaagat
     8341 cgctaaaaac cagtagtggt tgttgaattg aaaacatagt tgcgcagtac tccccttacc
     8401 aggatgattc tcaaaacagc acaaattatc aaagatgatt ttttcgggag cgatcccgct
     8461 gacgccagag ccaccaaggg gtgacgtgcg tcacatttct attcataagt agcgttaatc
     8521 attcatatgg cgaatatttt cttgtcagcg aaaaaaattg cggataaggt gatgaacaca
     8581 tcagatttcc tggtgtaacg aattttttaa gtgcttcttg cttaagcaag tttcatcccg
     8641 accccctcag ggtcgggatt tttttattgt gcattcaacg attcacttca gagacgctga
     8701 agtcatgaat atccagttcg aaggcatccg ccaattcacg ccatgtatga tattcacgtc
     8761 catcgacgct aacgtgttca gaatcgtgct ggctgcgatg aacttcgctt tcgctaattt
     8821 cattgatcgc cgccagcagg gcatcgacat cgacgctgac ctcacgtttt gcggtatcgc
     8881 tgtactcttt tgcggttttc atcatctttc ctcgcaaccg ttactctgtt gttgccgcca
     8941 gtcgttaagt ccgcctggca tctcttaagt atagaccgtc gagaaaatca gcactcgcgc
     9001 gcgcctggcg ctgccagaaa aacgaaattg ttctacactg gcacaaagcc acaggaggaa
     9061 aacgatgaag gtgaatgatc gggtaacagt caaaacggat ggcggtccgc gtcgtcctgg
     9121 cgtggtactg gcagttgagg agtttagtga aggcacaatg tacctggttt cgctggaaga
     9181 ctacccgctc ggcatctggt tctttaatga agcagggcat caggacggta tctttgtgga
     9241 gaaagcagag taatcttatt cagcctgact ggtgggaaac caccagtcag aatgtgttag
     9301 cgcatgttga caaaaatacc attagtcaca ttatccgtca gtcggacgac atggtagata
     9361 acctgtttat tatgcgtttt gatcttacgt ttaatattac ctttatgcga tgaaacggtc
     9421 ttggctttga tattcatttg gtcggagatt tgaatggttc cctgacctgc catccacatt
     9481 cgcaacatac tcgattcggt tcggctcaat gataacgtcg gcatatttaa aaacgaggtt
     9541 atcgttgtct cttttttcag aatatcgcca aggatatcgt cgagagattc cggtttaatc
     9601 gatttagaac tgatcaataa attttttctg accaatagat attcatcaaa atgaacattg
     9661 gcaattgcca taaaaacgat aaataacgta ttgggatgtt gattaatgat gagcttgata
     9721 cgctgactgt tagaagcatc gtggatgaaa cagtcctcat taataaacac cactgaaggg
     9781 cgctgtgaat cacaagctat ggcaaggtca tcaacggttt caatgtcgtt gatttctctt
     9841 tttttaaccc ctctactcaa cagatacccg gttaaaccta gtcgggtgta actacataaa
     9901 tccataataa tcgttgacat ggcataccct cactcaatgc gtaacgataa ttccccttac
     9961 ctgaatattt catcatgact aaacggaaca acatgggtca cctaatgcgc cactctcgcg
    10021 atttttcagg cggacttact atcccgtaaa gtgttgtata atttgcctgg aattgtctta
    10081 aagtaaagta aatgttgcga tatgtgagtg agcttaaaac aaatatttcg ccgcaggagt
    10141 atcctggaag atgttcgtag gtattatttt tcggataatc cttaggataa catgataaac
    10201 gttacggaat tatattaatg gcaattcact aataatatca gccagcaaat taaacatctc
    10261 actgaataaa tgttcgcaaa aaggcgcaat taacggcatt aatgccgcca ttaaagagat
    10321 accgacagtc agagttaatg gaaatccaat aacaaaaatg gataattgcg gggccatacg
    10381 attaagtaaa cctaatgcca gattcagtgt cagcagcaga gtaatgagcg gtaacgccag
    10441 catcagcccg ttaaggaaaa tcaaactccc tgctttggtg agtgccagaa acgcattgct
    10501 gttcaacggt tcgccaccaa tcggcagggt gtgaaaggta tcgaccagca gtgaaatcaa
    10561 ccataaatga ccgttaaatg tcaggaacag cagtaacgcc agcatatcca tgatacgcgc
    10621 taaaacgggc atattgagat ggctggccgg atcgacaaac gtcgcaaatg acagccccat
    10681 ttgcagaccg ataatttcgc cagcggttcg cacagcggca aaggcaaatt gcatggtaaa
    10741 accaagcgca atgccgatca ggatctgctg cacggccagc cacagagcaa agaacgaaaa
    10801 aacaggaaca tcgttggcag gtaatgatgg ggcaatggcg aacgtgatca tcattgccag
    10861 ccccagtttt acccgtttcg gcacgctgcg ttcgctcaga attggtgcgg tagaaatgag
    10921 cgccagcacg cgcagtaacg gccagaagta caggcttaac caggatagcc attgttcgct
    10981 tgtcacctgc aacatagtac ggctacccga tgatatacgg caggttagtg aacaaggtgc
    11041 ggacgtaatc cagcaacaga ttgagcatcc acggtccggc aataataatg gcgataaata
    11101 cggcgatgat tttcggaata aacgacagcg tcatttcgtt aatctgcgtg gcagcctgca
    11161 aaatactgat gataaggccc gtgaccaacg ctaccaacaa tagcggggca gccagtgcca
    11221 gcgcgacttt catcgcttca gtccccatca tcatgaccga ttcaggtgtc attttgcctc
    11281 tctagctgta aaagctctgc gccagcgaac cgaccagcaa ttgccagcca tccaccagta
    11341 caaacagcat cagtttaaag ggcagagcaa tggtggcggg gggaaccatc atcatcccca
    11401 atgccatcaa cacgctggct atcaccaggt cgataatcaa aaaagggatg aaaatcgtga
    11461 agcctatctg aaatgcggtt ttcaactcgc tggtcacgta ggccgggagc aaaatgcgca
    11521 tcggcacggc ttcaggtccc tgcaacgggc cggtattcgc cagtctggca aacaacccta
    11581 aatccgcctc acgggtctga cgcagcataa actcacgcag cggctgcgcc cctttttcca
    11641 gcgcctcctg cattgatatt ttctcttcgc tgaatggctg gtacgcatcg acataaattt
    11701 tgtcgatcac cggtgacata ataaaaaagg tcaaaaacag tgccagcccc agcaatacct
    11761 ggttaggtgg cgcggagggt gttcccagcg cgttacgcaa taaaccaaaa acaataatga
    11821 tgcgggtgaa actggtcatc atcagtaaaa ttgccggaat aaacgtcaac gaggtgatga
    11881 acaccagcgt ttgcaccggg agcgaccagc tttgtccacc gccaggcagc ggctggctgg
    11941 tgatacccgg cagttgcgcg aaggcgaggg gcgtaatcag ccagagaagg acaggtgcga
    12001 cagacaataa acgacgcatc aggatctccc gctacgctta agcaaatttt tcatgaccga
    12061 ctgaaaatcg gtctgcggta tctcttccgt tggtgcagaa gggggaagtt tatgcagcag
    12121 attgatttgc cctgcggtaa cgccgagcac cagccgtgca tcttccacat cgaccaccac
    12181 aacccgttca cgcgcgccca gcgaagcact ggcactaatt ttcagaccgt taacgccagt
    12241 tcgtttaggg gcaaatccca gccgttttac tagccaggca gcagcgagga tcagggcaat
    12301 aatggcgatg agtgcgccgc tcacctgcag cagtggcgca gcagaaatcg gcgcggaaga
    12361 ttgcacagta gcgtggttat tcatcactaa cggctcaggc gacgcattcg ctcagacggg
    12421 gtaataatat cggtgatccg cacgccatat ttatcagcga ctacgacaac ttcgccctgg
    12481 gcgattaaat aaccgttgat cagaatatcc agtggttcgc ccgccagacc gtccagcgcc
    12541 acgacggacc cttgcgtcag acgcaacagc tctttgatgg tcatccgcgt acggcccagc
    12601 tcgacggtca gcttgaccgg aatatccata atcaggtcga tatcctgcaa cgttccgctg
    12661 acatcaccac cgccaaattg ctggaacacc gtgtcggcag cgcttttgct actggttgat
    12721 ttttgttcgc tcaacgcttc agcccacaga tcgtccattg cgccgttgtt gtcatcggcc
    12781 ggattattca tgtcactcat ttgggctgtt cctcgttcag agaatttaaa atcgggttaa
    12841 tcaaatgttc tatccgtaac gcatactgac cgttgagggt gccgtactga ctggtcagca
    12901 ccgggacgcc gtcaacatgg gcgatgatgc gatcgggttt ttctatcggc aggacgtcac
    12961 cgggtttcag ttttaaaatc tgcgacaggc gtagcgagat atcggcaaag ttggcgacca
    13021 gctccagctg tgaatgctgc acctggcgca ccaggttatc gcgccagttc tgatcttcat
    13081 tacgcgagtt ttccagcggc gggttaacca acaattcccg tagcggctcg atcatgctga
    13141 aaggcaggca gatattaaat tcgccggtca ggttgccaat ctccacatgg aacggcgtgt
    13201 taaccacaat gtcgttcggc gaggtggtga tattggtaaa tttcacctgc atttccgaac
    13261 gcacgtactc aacttccagc ggattaatcg ccttccatgc gtcgctatag ccttcaagcg
    13321 ccagtttcaa catgcggttg atgacgcgct gttcggtatg ggtaaactcg cgaccttcca
    13381 ctttggtcgg gaagcgtcca tcgccgccaa acaggttatc cacggcgata aacaccagac
    13441 tcggcgagaa caccaccagc cctgtgccgc gcagcggttt cagatggata aggttcaggt
    13501 tggtcggcac cggcaggttg cgggcaaatt catggtacgg ctgaatgcgg atggccccga
    13561 cggtaatatc cgggctacgg cgcagcaggt tgaacagccc catacgaaaa tggcgagcaa
    13621 agcgctcatt aatgatttcc agcgcctgca aacgttcgcg cacaacccgt cgttgggtat
    13681 tcggatcgta cggacgaatg tcactttcgc cgctaacact ggctgtcggt tcgtctttga
    13741 cttcgctgtc accattcaac agcgcatcaa tttcagcttg agaaagaata ctatcgccca
    13801 tgtcgttatc gcagaataaa agcggtatac agcacgtcgg tgacatcctg tttcggttgc
    13861 ccggcaacaa gcggggtgga aagcgtggtt ttaatctcgg caatcaggtt tttcttgcct
    13921 tcttctgtcg ccagtacggc agcatcctga cgcgaaaaca gtaacagcaa gcgactacgg
    13981 acttccggca aatactcact cagccgcgag cgggtagctt catctttcag gcgcagggtt
    14041 atgccgatat aaagcacgcg atccgcatcg cccagattga ccgtaaaggt atccagcgca
    14101 tagaagaccg gtgagggcac gacgcgttgc tgcgctttgt cgtcagcggc aacctgatgc
    14161 gaatgccagt agctgtaacc tgcgctggca caggccgcga gggtaatgaa taccagaatc
    14221 gggatccaaa gcgatcgctt gcttttcttg cttatcgcgt aatcagtcat gtgttgcggt
    14281 cttcctgtgt cgctactgct tatcggctaa ttatcctgcg tcttgagcgc aacaaagcgg
    14341 ggaaaagacg cggattacgg tgctacctct gacgttaggc gaaaatatca acgccgctgt
    14401 tgcctgttac acgcccttgt aaagagacgg gaaccggaag cgtatcgtcg tcttcccccg
    14461 ccagaggttc atggtttact gtgcgttggc tttgctgttg ttgggaagcg gcctgctgct
    14521 gaccactaaa gctttcgcca ctgatgttgc tttgccctaa ctgaatgcca ctttcggcca
    14581 gctgcgtacg cagtaccggc agcgctgctt ccagggcggc gcgtacatgc tgatggggtg
    14641 aaaccatctg gatttgcgcc tggttatcat ccactttgag ggagatttgc acttcaccta
    14701 aatcctgcgg gtgcagacgc aactctgcac tttgttgccc ctggcgggtg aacagcgaaa
    14761 tatgctggct taatgattgt tgccattcgt gagaacccag cggtgcactc aaaacaggcg
    14821 cggcgacggt gggcagtggc tgtgtctggt gtggagcgat tagcgggctg gcggcagcgg
    14881 tcaccggtga aggtgtgctg atgacttccg ctttactctg ggcttctgct accagcggtg
    14941 ttaatggctg agctggcgtg ccgggggcgt catcaggctg tgctgttgtg agttgcgtag
    15001 aagtcagttt tgtgaagagc gttggtttct ctgtcggtaa cacggttgac ggcgcatcag
    15061 tcactttggg cgtattgtca aaacccggca acatcgcaaa aagggcactc aggcttgcgg
    15121 tgacgtcttc attcagatca tccgcttttt cgtcttttgc cgtattgttg ccagccaccg
    15181 cagccaacgt catcgtttga gcggtggtta atggtgttga tgtggattgt tcgtcgttga
    15241 tgacaggcag tgtttcatcc acggggatca gtaaatccgc ttgttgcgcg tcggaaacaa
    15301 tatcgctgac cagcggctcg ccttttgtcg tgggcttatc tgttgccacc agcaactggg
    15361 gagtcgcttt gtcggttgtt gcctcgcctg ttaatgcttc gctcaacaac gcgagaaaat
    15421 cttgtgcagc atcgctggct ttgccgccag acaatgtggt gttatcaacg tcagcggtaa
    15481 tcaagggcgc tatgcgaatc attcaggttt cctcgtggcg gcgcgctggg cgaactcatc
    15541 catctttttc tgatcgaggc ggttttctgc aagcagtgcc gccgtggatt gccgttcctg
    15601 cagtgtctgc caggcctgca aacgttgttt cttttctcgc cagctgttca gggcaatgtc
    15661 aactttctgc gtccactgat taagttgctg gcgatgctga gtaatggctt tttccagcgt
    15721 ctggataaac tgctgatagt tgatccagcg gttgctggtc atcccggcac tcatatcgct
    15781 gttgaggtta ttgcgatatt cattctgata atcgatcagc attttgagct gttcttccgc
    15841 ctgctgacat ccgcgacgca tttcacccag caggcgcgcg gcatcctcta cctctttttc
    15901 tgccagatct ttcagggtcg ccagcgcgcc atgttctgtc atctgccgtt atctcctggg
    15961 ttatgacact gtcgggaaaa tacgctccag cccctggaga gacgcttccc agtccgcgcg
    16021 ttcaaaaatg ccttgttgca aatagccctc cagctgcggc cacagggcga tggctttatc
    16081 gagcatcgga tcgctgcctt tggcatacgc gccgacgcta accagatcgc ggttacgctg
    16141 aaaactcgac aacagctgtt tgaaggtgcg cactcgcgcg taatgttgct cactgatcaa
    16201 cgccgtcatt gcgcggctga tcgacgcttc aatatcaatc gccggatagt gcccggcttc
    16261 cgccagtcgg cgagacagca caatatgacc gtcgaggatg gcccgcgcgg agtcggcaat
    16321 ggggtcctgc tggtcatcgc cttcggtgag cacggtataa aacgcggtaa tcgagccgcc
    16381 gccgctaatg ccatttccgg cacgctcgac cagtgccggt aatttggcaa acaccgacgg
    16441 tggataacct ttggtggcgg gtggttcgcc aatcgccagc gcaatttcac gctgggccat
    16501 cgcgtagcgg gtgagggagt ccataatcag caacacatgc tgaccacgat cgcgaaaatc
    16561 ttcggcaatg cgcgtggcat aggcggcacc ttgcattcgc aggagcggag acacatccgc
    16621 cggagcggca atcaccactg atcgtgcacg cccttcggca ccgaggatat tctcaataaa
    16681 atcttttact tcgcgcccac gttcaccaat caaacccacg acaatgacat cggcgcgggt
    16741 gtaacgggcc atcatcccca gcagcacact tttaccgaca ccagaaccag caaacagacc
    16801 catacgctgc ccgcgcccaa cggtaagcag ggcattgatt gggcgcacgc cggtgtccag
    16861 cacatgttca atcggtgtac gttgcaatgg gttaaatggt ggggtaatca gcgcaccggt
    16921 ttccgtcgta tcgggggagg gcaggccatc gagcggttta ccgctgccgt ccagaacgcg
    16981 acctaataac gccggaccga gcggcaactg tttgccgctt tgtagccctt caggcgaaat
    17041 gtttttggca taaacacgcg cgccgggcag gacaccttcc acttcctcca gcggcattaa
    17101 aaacagccgt tgaccgttaa agccaacgac ttcgctttct acttcgtgcg tttcgctgcc
    17161 gttctggcgc tcaatgacac aggttgcgcc gagcggcaat tgtaatccgg tggcttccag
    17221 caccagcccg gtagcgcggg ttaatcgccc gtagcgacgt accgcaggca actgcgccat
    17281 tttggcttca aagttatcca gcgtggttag ccagcgagtc aggcgcgtgg tcattacacc
    17341 actcctggtg ctgccagacg gcagagttct tgccagcgag tggcgacact ggcgtcgaga
    17401 tcgccttcat cggcggagac tttacagccg ccaggatgga gggtgggatc gccccgcaag
    17461 cgccagccat gcaaacttaa ggtagcgccg agcatatcat ccacacgttg cagatcatcc
    17521 gggtgcacgc gcagctgtgg tttaccgctg aataacggtt cttgctgcaa caactgttgg
    17581 atctgtttga tcagtgccga gttatccact gttggcgtct gaccgatgac ctgacgtgcc
    17641 gcctctagcg ccatctgcat caggcgcgac gctatcacac tatcaagtgc atcaagggta
    17701 gtttgaaatt cgctgaccag ttgctgcatc cgggcatgaa ttggcgcttg ttgagacttc
    17761 gcctctgcca gaccttgctc cagcccctga gccagtcctt cctgatagcc ctgcttatga
    17821 ccttgctggc gaccttcggc aatccccgcc tgataacctt gctcgtgggc ctgcatttgc
    17881 agttgcgcca gttgctgctc aaggctgggt tcggtctctt caatgatggt ttcttccggc
    17941 tcaactattg gcacaaactc tgcctgtggt ggcgcgagat cgtccggcgt ccaggttttc
    18001 cagggcagat tatcagacat aggtatcctc gccgctgcca attaccatct cgccagtttc
    18061 ggcaaggcgg cgcacaatca gcagaatcgc tttctgttcg ttttccacct gcgacagacg
    18121 caccggacca cggttggcga gatcgtcgcg cagaatatcg gcggcacgct gcgacatatt
    18181 gcgcaggaat ttctcgcgca gtggctgctc ggcccctttc agcgcgatca acagcgattc
    18241 ggaatccact tcctgcaaca gacgctgaat gctgcgatcg tcgacatcca ccagattctc
    18301 gaacaggaac atctcgtcga tgattttctg cgccagttcg ccgtcgaatt cacgcacggc
    18361 ggtaataacg gcttcttcct gctgagtttt catcaggttg ataatttcgg ctgccgttct
    18421 cacgccgccc attttgctgc gcttgagatt ctgaccgtcg agcaagccat tcagtacttc
    18481 ggtcagctct gccagcgcgg ctggctgcac gccgccaaag gtggcgatac gcaacatcac
    18541 gtcgtggcgc agacgttcat cgaacaacgc cagaatatcg gcggcctggg cgcgcttcag
    18601 atgcaccaga atggtggcga taatttgcgg atgctcatcg cgaatcagat cggcggcgct
    18661 ctgtggctcc ataaagttga gcgtttcaat accgctggcg gtatcgcgag tttcgagaat
    18721 atcttccagc aggctggcgg cacgttcttc acccagagct ttgaccaata ccgaacgcag
    18781 ataatcgttg gcgttgatat tcagtgcggc aaactgttca gcttcttgct caaactccgc
    18841 cagcacatcg gttagctgct tgttggagat ctgcgtgacg ttcgccattg cagcgctcag
    18901 ggtttgcacc tcgcgctggg agaggtgctt gaacacctct gccgcccggt cttcgccaat
    18961 ggtcatcagc aggatgacgc ttttatcggt gcctgtcagg ttactcatga tcgttattta
    19021 tccactggcg aatgaccagc gccaccacgc gcggatcgtt atcagacatt tcacggatac
    19081 gctggctcat gacttctgcc cccagacgtt ggttagcgcg ccgttgttgg agttgttcgt
    19141 ctttgctcag gcggacttcc accgcatctt ccacttcctc gcgggcctgc gcctgttgct
    19201 gtacagcttt catcgcctca gcgcgacgtg ttaactgcgg gcgtaccgct ttacgccaca
    19261 gcagccacgc caccagcagt accagcaacc agcgaccggc agccagtaac tgatcgataa
    19321 acgcttgctg ttgccagaac ggcagttctc cgccgctttc ctcactacta ttgaacggcg
    19381 agttaacgac attgagcgag tcaccgcgtt tttcagaaaa gcccatcgcc tcgcgggtca
    19441 gatcttcaat ttgcttcatc tgttcgttgc tgagaggcaa cggtttaccg tctggcaagg
    19501 ttttgtaatt caccacaacc gcgactgaca gacgttgcac atcgcccacg ttcattttgg
    19561 tatgacgaat ggtgcgatcg acttcgtagt tactggtttc attccgctgt gtgctacgcg
    19621 gcccgctatt gctggtggtg ctcgcctgct gctggcggtt attttgattt gccggaggcg
    19681 tgctgattgg cgcgttattc gcaggtgccg gttgattcga caacgccccc ggtacgccgc
    19741 ccggataacc ggaaccgctt tgctcgctct cattaagctg gcgtgaacga agcgccgcct
    19801 gagattcatc accgttaggg cgatactgtt cttccgtttg ttctttactg gcgaagtcca
    19861 gctgcgccgt aacctgggcg tgaatattac cgttaccaac aataggcgac aggattgctt
    19921 caatacgccg ctgaatacgt ccttcgacat cgctggcata tttcaattga gcgtcattaa
    19981 gatcgcgccc gctggtattg gactgggtta acagatgtcc gccctgatcc accagcgtga
    20041 cgtttcccgg cggcagacca gcaacggcgc tggaaaccag atgcacaatg gcgctaattt
    20101 gcccttcatc gagtgcgcgg ccgggtaaca gatttaccgt caccgatgca gaaggggatt
    20161 tttgttcacg gacgaataaa gacggtttcg gcattgccag atgtacgcgc gcccctttta
    20221 ccgggccgat agtttcgatg gtacgagaaa gctcgccttc cagcgcccgc tgataattca
    20281 cctgttcgct gaactggctg ataccaaact tttcctgatc aagcagttcg aaaccgaccg
    20341 cgccgccttt tggcaaacct tgttgtgcca ggcgcagacg cagttcgtga actttatctg
    20401 ccggaacttc aatagcgccg ctggcttcgc tgaagcggta aggaatattc atttgcgtca
    20461 gttggctgac aattgcgcca ccatcctgat cggaaagatt gctgaataat gtgcggtagt
    20521 cgggggcttt cgcccacagg atcagtgcga ccatgaccgc cactgccgcg gaaccggcaa
    20581 caatcaatgg aattttcgga ttcgcacgca ggcgattaag ccactcaaga gattttgtct
    20641 gggctgcagt cgcattcatc gcgcacctcg tggctgatta tggacggcgt tattggcaaa
    20701 gtggaacaag actcactccc gggtcagcaa actcgaaaaa ttgacgctcc cattatttgt
    20761 gctttcgttt ttttctatcc gccaataaac ccgttttttt gttgctattt agcgcctttg
    20821 tcttattgac ttactggtag gctttgctac cagaaattat ccaggagatg aaaatgtcag
    20881 cgatacaggg gattgaaggg gttatcagcc agttacaggc tacggcgatg agtgcgcgtg
    20941 cgcaggaatc actgccgcaa ccgaccatta gttttgccgg gcagctgcac gccgcgctcg
    21001 atcgcataag cgatacacaa acagccgccc gcacgcaggc agaaaaattc actctcggtg
    21061 aacccggcgt ggcgttaaac gatgtgatga ccgatatgca aaaagcctca gtttctatgc
    21121 aaatggggat tcaggtgcgt aataagctgg tggcggcgta tcaggaagtg atgagcatgc
    21181 aggtgtaggt tttgtaacct gttgttaatt acatccgatc tcaccgcgtg gggcatggat
    21241 ggggcaaact cactcaattt ctggtttaaa atggaaacct gattctgaaa gttttctgcc
    21301 atccacgatc cgtatacgcg ataaacctta tgatcatcag aatgtctcat ttgacacaca
    21361 tcaccacgtc ggatgttgtt acgaccacag acgcaacagt aatctctgga taatttaatc
    21421 ttcgttctta tttttattaa tatactgcgt tata
//
LOCUS       DAZFKF010000085        21375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFKF010000085 DAZFKF010000000
VERSION     DAZFKF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.085"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..98)
                     /locus_tag="ACHKA1_003727"
     CDS             complement(<1..98)
                     /locus_tag="ACHKA1_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO5681322.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNT"
     gene            complement(314..694)
                     /locus_tag="ACHKA1_003728"
     CDS             complement(314..694)
                     /locus_tag="ACHKA1_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5681323.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSATKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            complement(712..1701)
                     /locus_tag="ACHKA1_003729"
     CDS             complement(712..1701)
                     /locus_tag="ACHKA1_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5681324.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSGWHSE
                     NMTTMRLAPGAIRLCWHCDNLLRDQFTERLEAMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIRDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            complement(1753..2011)
                     /locus_tag="ACHKA1_003730"
                     /pseudo
     CDS             complement(1753..2011)
                     /locus_tag="ACHKA1_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2008..3408)
                     /locus_tag="ACHKA1_003731"
     CDS             complement(2008..3408)
                     /locus_tag="ACHKA1_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO5681325.1"
                     /translation="MTNPTNPAWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFF
                     VRQYRDIYAGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWAKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAHTCEAAEDALKDAQNMMTSLSTGKGVIQPVHIDDVL
                     PEVVDRVECRNQGLEKSRALMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRHIWMVDQANLTIDEICATTRHHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPETQARGIAEINITKQRNGTQGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMSGSKAERLF"
     gene            complement(3405..4295)
                     /locus_tag="ACHKA1_003732"
     CDS             complement(3405..4295)
                     /locus_tag="ACHKA1_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5681326.1"
                     /translation="MTNFLNKQHMQHDKAQLLNRKADLEEELAFVRGGKRPWRCEHWV
                     QSTETVSCGKHGKYTRYVLTGPDVRGKAVKRVSGCLSCLSEELDRVYDELRALKVREL
                     LDQAGIARRFQDCEFENYQAVNTDAQKNLEACQRYAASWKKCLAAGTSMVMIGNCGTG
                     KNHLAVSMAKNIIRHHQATIEITDVMRLTRAVKNTWRHNSERTDDEVINHFASLDLLI
                     IDEVGVQFGTPTEITILQEIINARYESVLPTILISNLTFEQLKESIGERIVDRVTDGG
                     RNCLVFGWESYRAHIRGVAA"
     gene            complement(4315..5226)
                     /locus_tag="ACHKA1_003733"
     CDS             complement(4315..5226)
                     /locus_tag="ACHKA1_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5681327.1"
                     /translation="MSSKILGNVWDACAAYGIKGAKLMIMARLADYSNDDGVCYPGVE
                     TICRQLGLGESTVRTAISELEADGWLTRQSRRKGNRNTSNLYHLNADRLELLARTERD
                     KVAELKQQRRLSGLRDPSDSEPSKSEPSESVCSGVFDPSDSGKNTRLTLQNLTPDPQG
                     LKHEPPVNSKHEPQDIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFK
                     AWKARIREGVTPETMLNGVKRYAAWVRVSGNTGTQFVKQASTFFGPDRHFEESWQQPA
                     APGGGRGKSLPISGFSEQDYGSTNFNW"
     gene            complement(5223..5447)
                     /locus_tag="ACHKA1_003734"
     CDS             complement(5223..5447)
                     /locus_tag="ACHKA1_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681328.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGHVLRVCLVNIESQKVV
                     CQVQGRTHTLSYPLVAFQSGKMFKRLGGAV"
     gene            complement(5444..6307)
                     /locus_tag="ACHKA1_003735"
     CDS             complement(5444..6307)
                     /locus_tag="ACHKA1_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5681329.1"
                     /translation="MTRHQHIDSASPGRYTVPAPYKTGAGIGVLKLITEHNRAHAVFS
                     CHEHYYAQIMVGRTGPTSVGPGSVVSGNANPVRLTTSVIGVPCGEFSKKLTTGAVTMT
                     TLPTLSQPEIAIVDGQAVTSSLAVADFFSKRHDDVLKKIRILDCSPEFCARNFAETSI
                     LVRQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMEKSLSGADAS
                     DMSAVARNARGVYLHLREIHQIWTSQLYPMLKAVESPLASKLYDRVGDAVFGAALVDS
                     RLNGSDKEVRP"
     gene            complement(6304..6936)
                     /locus_tag="ACHKA1_003736"
     CDS             complement(6304..6936)
                     /locus_tag="ACHKA1_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO5681330.1"
                     /translation="MKFRRHTLHIVAKSTIGSETPELLQATNDAPASFLLSASLHARI
                     MVGWAGEPKGSPVSCNAGSLNPVQSATSEIETSGGGTVPLLQEAALMATIPAHPYLKI
                     EVVNGRAVIFSLYVACHFKRMHKNIIDRIEYLNCSREFLTRNFIPGTYRAYGDSVRGY
                     YITRDGLMMLQLGLSLRTMRYYESCIEAFHEAEAVQGHSVFRRNQREVHQ"
     gene            complement(6965..7141)
                     /locus_tag="ACHKA1_003737"
     CDS             complement(6965..7141)
                     /locus_tag="ACHKA1_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001664303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5681331.1"
                     /translation="MQVLNRWFRDGRGRRVHVIRWESETQRVIYLRDGYPHECFIPLW
                     LFRRDFVECEAPPT"
     gene            complement(7161..7868)
                     /locus_tag="ACHKA1_003738"
     CDS             complement(7161..7868)
                     /locus_tag="ACHKA1_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5681332.1"
                     /translation="MNTTIFTDKASMTSVEIAELVGKRHDNVKRTIETLAKGGVVRSP
                     QIEVSERINNLGFKVQHEHYLFEGEQGKRDSIIVVAQLCPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRLERVEEKVSHVTETVEQIKRGAIRDGYAGYRQLAAK
                     TGMSDAKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMSAFHRMTSEAEPRGT
                     RWYHPKMGLFQALGWQV"
     gene            complement(7865..8128)
                     /locus_tag="ACHKA1_003739"
     CDS             complement(7865..8128)
                     /locus_tag="ACHKA1_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5681333.1"
                     /translation="MKNEGIAKAVDIAGSQIALARRCGRAQSTICDWLNGKKKISPEF
                     VPSLVKAVDGKVQAYEFRPDLPELFPHPSLAHTEDGVCEGDKQ"
     gene            8252..8956
                     /locus_tag="ACHKA1_003740"
     CDS             8252..8956
                     /locus_tag="ACHKA1_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO5681334.1"
                     /translation="MSFSDRLQLAMTIRGYSQGKLAREVGMAQSSVNKLVNGATGSRK
                     VVEIANTLNVRPEWLSYGVGPMTPEESTSCNPTPQVSYSPLENDKDIFRVEVLDIAAS
                     AGPGTFLVSDFAETVHAIEFSHDAARRLFCSRPANIIKMITVDGDSMAPTLCAGDQVF
                     VDVSVRNFETDGIYIFIFGHTFHIKRLQMQGMQLAVISDNPAYKEWFISESAEEHLFI
                     MGKVLIHQSIQYNRVG"
     gene            9216..9803
                     /locus_tag="ACHKA1_003741"
     CDS             9216..9803
                     /locus_tag="ACHKA1_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5681335.1"
                     /translation="MSENGAESERVYNAIRCLMRSLQKTEETASIFAEKLCEAKAMVS
                     HGHFFGKIRAQREACGLTTTELAKLLDLDEEIILQWESGEFEPTISMLIPLANVIGCD
                     PLSLLDEKDSASVIRVNAPEVYVESIGARIKSARKKLGLTEADLARMINTYSDPINDW
                     ECGICEVPADQIVPLASALNCDLMWLLTGKSEAKE"
     gene            9809..10009
                     /locus_tag="ACHKA1_003742"
     CDS             9809..10009
                     /locus_tag="ACHKA1_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681336.1"
                     /translation="MTGNIHDKYEGLCLAPDSFANNIHDLLCAVVVLQMSDNDAIKRT
                     GDEVLEFARCYAEAAAEKELSS"
     gene            10208..10390
                     /locus_tag="ACHKA1_003743"
     CDS             10208..10390
                     /locus_tag="ACHKA1_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681337.1"
                     /translation="MHQKTAEHEQTRVLLTIKNGKVILIRHVHDDEFVGNLSTFLFIA
                     EKAGYDVIAPADEDEE"
     gene            10396..10968
                     /locus_tag="ACHKA1_003744"
     CDS             10396..10968
                     /locus_tag="ACHKA1_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681338.1"
                     /translation="MQYAEFQAEATANGIQTGSMTIDYHDAIRRLDAGEFDTPNVRGL
                     RILQCLAQADEAGLLGKLPVEMKVAQWRWLYVTTFINEEEDKNGTIDVPNEHGTTDRA
                     VIYNGKHGVMTIYPSPIRFALQQYIEWNLIQTYGEAEGMGRALFLYQKMLTTSPDKGF
                     ILSDIGREGLELLLDEMINDLNTHGMPEGQ"
     gene            11153..11341
                     /locus_tag="ACHKA1_003745"
     CDS             11153..11341
                     /locus_tag="ACHKA1_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681339.1"
                     /translation="MRLYFTIVILLAIIACIYGLLVPFLISMKDTRAVISGFALAFLT
                     PPCIYAIYKGLSFTKDKK"
     gene            11338..12165
                     /locus_tag="ACHKA1_003746"
     CDS             11338..12165
                     /locus_tag="ACHKA1_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO5681340.1"
                     /translation="MKKIIFALAIVLPTIGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDSALKDIQAEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSDANTPFIQVK"
     gene            12206..12577
                     /locus_tag="ACHKA1_003747"
     CDS             12206..12577
                     /locus_tag="ACHKA1_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO5681341.1"
                     /translation="MNTHNAQPQIMNYDPNLTSCGRMAKQTVRLTFGLWEYRETFEVT
                     VGGNLTGLDVISCAIESLYATLPYEEVEDERTGGTDIMATINIGELICQDEDLSGESW
                     LAGMLVAAEIISIEPATNIRL"
     gene            12609..12851
                     /locus_tag="ACHKA1_003748"
     CDS             12609..12851
                     /locus_tag="ACHKA1_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5681342.1"
                     /translation="MFDLINQGQLYTDSAGYPVKIIHCINNIVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQEAHLKKLRAMKRK"
     gene            12855..12989
                     /locus_tag="ACHKA1_003749"
     CDS             12855..12989
                     /locus_tag="ACHKA1_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681343.1"
                     /translation="MNKAFELWVRQCYGNRYDLTRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            13008..13262
                     /gene="xisR"
                     /locus_tag="ACHKA1_003750"
     CDS             13008..13262
                     /gene="xisR"
                     /locus_tag="ACHKA1_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO5681344.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGVKNASSG"
     gene            13296..14582
                     /locus_tag="ACHKA1_003751"
     CDS             13296..14582
                     /locus_tag="ACHKA1_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5681345.1"
                     /translation="MSNTSYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGNFDYAAQFPESPNLKHFGLGKKEITIKALSEKWLDLKKIEIGGN
                     AFNRYQSVVRNMLPRIGESRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPVKGRSVV
                     TVNYYMTTIAGMFQFAADNGYTESNPFNGLTPLKRSRTEPDPLTHDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAIETLKSQAEMTRFGTQYQIDVKLREFGRTVSHECTFVFNPQLVKKCQQAGY
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALAAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKELAESVPYMPHRARR"
     gene            14584..15363
                     /locus_tag="ACHKA1_003752"
     CDS             14584..15363
                     /locus_tag="ACHKA1_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO5681346.1"
                     /translation="MISFSIIELTHGSRHVNCVEGNTTLYALPKPEVVLRWREQTTDD
                     FRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALW
                     HFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHAARPHSE
                     AIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYL
                     FLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            15416..15811
                     /gene="yecN"
                     /locus_tag="ACHKA1_003753"
     CDS             15416..15811
                     /gene="yecN"
                     /locus_tag="ACHKA1_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO5681347.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            15852..16595
                     /gene="cmoA"
                     /locus_tag="ACHKA1_003754"
     CDS             15852..16595
                     /gene="cmoA"
                     /locus_tag="ACHKA1_003754"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO5681348.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            16592..17563
                     /gene="cmoB"
                     /locus_tag="ACHKA1_003755"
     CDS             16592..17563
                     /gene="cmoB"
                     /locus_tag="ACHKA1_003755"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO5681349.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(17728..20156)
                     /gene="torZ"
                     /locus_tag="ACHKA1_003756"
                     /pseudo
     CDS             complement(17728..20156)
                     /gene="torZ"
                     /locus_tag="ACHKA1_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
     gene            complement(20181..21281)
                     /gene="torY"
                     /locus_tag="ACHKA1_003757"
     CDS             complement(20181..21281)
                     /gene="torY"
                     /locus_tag="ACHKA1_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO5681350.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGFLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAVGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         5461 a         5464 c         5054 g         5396 t
ORIGIN      
        1 gtattctgcg caatctcccc gactgtcgcc ggttcggtga cgcttaattc attaaacacc
       61 actctggcgg tttctgtcat atcctgctgt ttcagcatgt ctttttccct tttccggtta
      121 acgtgacaca ccaataactc ttgtcgaaaa agccagcaag ctgaaagacc ggtattcacc
      181 gccaccagcg cgtttactgt actggcgtga tttcagtcat aaaaaaaccc gctcgcggcg
      241 ggtttaagct gtgtggcgaa gtaaccactc ttaacatact gacatacttt ttgcggaccg
      301 cactaatcat tttttacttt tttagcagcc agtcgtccat ctccagtctt acccccagca
      361 cagacaaaca tccgtcaata aacccttcgg ctatctgcat ctcaattcgt attgcttttt
      421 cgcttttctt tctcgtcctg gctatctgtc tttttgatat tcgcaacaaa taatgagcaa
      481 tgagaagcga atactcctca ggttttttct gcttcagacg agcaagacag ttttcaatga
      541 taagtccgtc atcatcgcag caggccggac gtggtttagt ggcagatggt aaaagtcctt
      601 tgaatccggc agcgatcgga gaatagtcca ccccggtgtt accacttgcc gcccatgccc
      661 cccagcgttc aagaaccatc tgaatatcac gcatcaactt tctccacaaa atcaggacag
      721 cacaccaatc gccagtgcgc gatcgataaa acgaaatatc agctccagtt gggaaccata
      781 cttctcttca aatgccacag tatccgcatg cagttcgtcg tggtgttttc tgcacaaagg
      841 cagcacaaaa aggtcatgcg cttttgtccc cataccaccc tgaccgtgac caatcagatg
      901 atgcggatca tcggctggct ttccacaaca cgcgcacggc tgcgtcttaa cccagcgagt
      961 gtacttttca ttaacccagc gacgacgttt cgggcgtaac ataaaagact ccggcgactc
     1021 cggatccact ttcagcgcca gcaccttttt cgctttatcc cggatgatgc tggtggcggg
     1081 aaccgaaggc acaaggtcac tttcccgggt gacagacggc acaacaggct tcggtaatct
     1141 cagtgcctta cgggctgcac tttccggtaa ggcatccgcc aggtcattac gaaccagcca
     1201 ccagcacagt tccggcattg tcacaacgtg actgtcatca aaaccaagat cacggcgcac
     1261 aacagacaac acccagcggg cacagttatc cgttgccatt gcttccagcc gttccgtgaa
     1321 ctggtcacgg agaagattgt cacagtgcca gcacagacgg attgcacccg gcgcgagccg
     1381 cattgtggtc atgttctcgc tgtgccatcc ggaatgaggc cactgacagc ccttttcacg
     1441 aagtaaccag ctttcaagac attccacccc accagcacga cgaataactg cctcattgcg
     1501 gaacacggcc cgcacagcag gatcatccgc taacggttgt gatgccgccg gaacggcacc
     1561 actggcaaaa gatgaataac gttccggctc aggctccagc aggacacgcc cctgcataaa
     1621 caggggcatc agctctgagc ctggtctgaa cagtacgatc cccattcgcg gggcaatttc
     1681 aggagtcaac agtgctctca cggtcacctc agcgaacgat attgcatgaa cacaggagaa
     1741 aaaattcagc catcacgcag taaactcctt caccagcgtt tcaaactggc ttaccttgcc
     1801 ttccagttcc gccacgcaat ccaccagctc atccactgcc ttttgtgtgc ggtgttttgc
     1861 ctgcagcaga tcacgaagcg ccggagtaag ctgcttgcgg agcgtatcct ttgccacgct
     1921 catttttttc catctgttca gcacaacgaa gcatctcctg cgcctggcga cgaagttgtt
     1981 ccggtgaaac agtggctgtt ctgttattca aaataaacgc tccgctttac tgcccgacat
     2041 gcggttattg ctgtatctgc gcggattgcc cggcgtcatg ggtgtggaaa gaacccgggc
     2101 actctcctgg tccacaggca gaaaatgccc gttatgaaaa cgccggtaaa tggtaccctg
     2161 cgtgccatta cgctgtttcg tgatgttgat ttctgctatg cctctcgcct gtgtctccgg
     2221 gttgtacacc tcatccctgt aaagcatcag aatgatgtct gcatccgcct ctatttcccc
     2281 ggagtttttc aggtccgagt tcatggggcg tttattgggt ctggattcca caccgcggga
     2341 gagctggctc agcgctatca gcgggaaacc gccggatttt gccaggcttt taagtccctt
     2401 tgagatttcc cccacagcaa ggtcgtgacg ccccgtacta cgggttttaa tcaggccgag
     2461 gtaatcgacc accaccagcg ccgtttccgg gtgtttcatc cggtggtgcc tcgtggttgc
     2521 acatatctca tcaatggtca ggtttgcctg gtccaccatc cagatatgac gccccgtcat
     2581 tcgtcccacg ccctgtgaga aacgcgccca gtcttcatct tcaaaacggg caacagactt
     2641 aagacgggat accggcattc ccccggcagc agacaccata cgttcaccaa tctggatgtt
     2701 cgccatctcc atggtgaaca gaagcacgcc atgcccctgc tcagtcacct tgtcgatgat
     2761 atccagcgca agttcggttt tccccatcga aggacgagcc gcaatgaata ccaggtcgcc
     2821 tggctccata ccgcccgttt ttgcgtccag ttcatcaata ccggtcatca gtgccctgga
     2881 tttctccagt ccctgattgc ggcattcaac acggtcgacc acttccggaa ggacatcatc
     2941 aatgtgaacc ggctgaatga cgccctttcc ggtcgacagt gaggtcatca tgttctgcgc
     3001 atccttcagg gcatcctcag ctgcttcaca ggtatgcgca tcacgtaaat tctgtaatgc
     3061 ttcggtcagt gttttttctg catcgcgcag tgcggcattg cgccgcagcg ctgcaacata
     3121 gtgctccagt gaagacttcg cccaggtttt gcgtccggtg tcggtaatca ccggggcaag
     3181 ttccggcatc tcattgcaca gcagtacggg gtcaatgacg ccggatacac gggcctgtct
     3241 gcaaattccc gcgtaaatat ccctgtactg acgcacgaaa aagacatccg ccggaagtgt
     3301 ggccagaata tccatcactt ccggatcagc cccacgcaga aaaaacgcgc caatgacagc
     3361 tccttccagg tcatcattac gccacgccgg atttgtcggg tttgtcatgc tgccacacct
     3421 ctgatatgcg cacggtagct ttcccaacca aacaccaggc agttacgccc accatcagta
     3481 acgcgatcca caatccgttc accaatggat tccttaagct gttcaaacgt caggttgctg
     3541 atcaaaattg tcggtaaaac gctttcgtaa cgcgcattta tgatttcctg caggatggtt
     3601 atctctgtag gcgtgccgaa ctgcacacca acctcatcga tgataagcag atccagcgat
     3661 gcaaaatgat taatcacttc atcgtcagtg cgctcagagt tgtggcgcca ggtatttttc
     3721 accgcacggg taagccgcat cacatccgtg atttctatgg ttgcctggtg gtggcgaata
     3781 atattttttg ccattgatac agccaggtga tttttcccgg taccacaatt gccaatcatg
     3841 accatgctgg tgcctgcagc gagacatttc ttccaggaag cggcatagcg ctgacaggct
     3901 tcaaggtttt tctgtgcgtc agtattcacc gcctgataat tctcaaactc acagtcctgg
     3961 aaccggcggg caataccggc ctgatcgagt aattcacgaa ccttcagggc gcgtaattcg
     4021 tcatacacgc ggtccagctc ctcactgagg caggacaggc aaccggacac ccgtttgaca
     4081 gctttccccc ttacatccgg gcccgtcagc acatagcgcg tgtattttcc gtgtttcccg
     4141 caggacaccg tctcagtgct ttgcacccaa tgctcacagc gccacggacg ctttccgcca
     4201 cggacaaatg ccagctcctc ttccaggtct gccttgcggt taagtaactg cgctttgtcg
     4261 tgttgcatgt gttgtttgtt caggaaatta gtcattttca ccctccccac aaaatcacca
     4321 gttgaagttc gttgagccgt agtcctgttc actgaatccc gagatcggga ggcttttgcc
     4381 ccgcccacct ccgggggctg ctggctgttg ccaggattcc tcgaaatgac gatcgggtcc
     4441 aaagaacgtc gacgcctgct tcacgaactg ggtaccggta tttccagaga cacgcaccca
     4501 ggcggcatag cgtttcacac cgttgagcat ggtttcgggt gtcacacctt ccctgattcg
     4561 ggctttccag gctttgaagg ctgctgactt ggaattacca ccagcacgtt tgggatattc
     4621 ctgccaggcc tgttcaaatt ccggtgaata ttcctgtctg gcagaacgcg ctggtgcaga
     4681 cgcgtcagcg gatgcgccaa tatcttgcgg ttcatgtttt gaatttactg gtggttcatg
     4741 ttttaaacct tgtggatctg gggtcagatt ctgaagggtc aaacgcgtat ttttgccaga
     4801 atctgaaggg tcaaacacac ctgaacatac agattctgac ggttcagatt ttgaaggttc
     4861 agaatctgaa gggtcacgta atcctgaaag tctgcgctgc tgtttcagtt ctgcaacctt
     4921 atcccgctca gttctggcaa gcagctcaag ccgatcagca ttcagatgat aaagattgga
     4981 cgtattacgg ttacctttgc ggcgtgactg acgcgtcagc cagccatcag cctccagttc
     5041 ggagattgcc gttctgactg tactttctcc caacccaagc tgtcggcata tggtttcaac
     5101 acctgggtag cacaccccgt catcattcga ataatccgcc aagcgcgcca taatcatcag
     5161 ttttgcacct ttgatgccat atgctgcaca tgcatcccag acattaccta ggattttgct
     5221 actcatacgg cacctcccag acgcttaaac atttttccgg actgaaacgc caccagcgga
     5281 taactcaggg tatgagtacg tccctgaacc tggcagacaa ccttctggct ttctatattg
     5341 accaggcaaa cccgcagaac gtgtccgttg ctggtggtga accactgccc cacacggggg
     5401 caatggttgt atcggtgata caggaaatta acgatgtggc ggatcatgga cgcacctcct
     5461 tgtcagaacc attcagcctg gaatcaacaa gtgcagcacc aaaaacagca tcacctacac
     5521 ggtcgtacag tttgctggcc agcggagatt caacagcctt aagcattgga taaagctggc
     5581 ttgtccagat ttgatggatt tcacgcaaat gcaggtatac gcctctggcg tttcgtgcga
     5641 cagctgacat atcagacgca tcggcacctg ataaactctt ctccatctgg ttaaaggcat
     5701 tgatgtatgc ctctttgaac cgggcagcac gtttaccagt gaagcccata gcaagaaacg
     5761 caaagccgtc gcgggttatt tgatagcaag gaagtttgcg agtaccgccg ttgggctggc
     5821 gtaccaaaat tgatgtctcc gcaaaattgc gggcacaaaa ctctggagaa caatccaaaa
     5881 tgcggatctt tttcagaaca tcgtcatgac gtttagagaa gaagtcagca acagccaaag
     5941 aagatgtaac agcctgacca tcaacgatgg caatttcagg ttgagagagg gttgggagag
     6001 tagtcatagt gacagccccg gtagtcagtt ttttagaaaa ctcaccacat gggacgccaa
     6061 tcacagaggt ggtgagacgt acagggttgg cgttaccgga gactaccgaa cccggcccga
     6121 ccgaagtcgg ccctgtacgc cccaccataa tttgggcgta gtaatgctca tgacacgaaa
     6181 aaaccgcatg agcgcggtta tgctcagtaa tcaatttcag gacgccaatc ccggcacccg
     6241 ttttataagg tgccggaaca gtgtaacgtc ccggacttgc agaatcaata tgctggtggc
     6301 gcgtcattga tgcacctccc gttgattacg acggaacacg gagtgaccct ggacggcttc
     6361 agcctcatgg aatgcttcaa tgcagctctc gtagtaccgc atggtgcgca gacttaaccc
     6421 aagctgtagc atcatcagac catcacgggt gatgtaataa ccacgcactg aatcgccata
     6481 ggcgcgataa gtgccgggaa taaagttgcg ggtgagaaat tcgcgtgagc agttgagata
     6541 ttcgattcga tcgatgatat tcttatgcat gcgcttaaag tgacaggcga catacaggga
     6601 gaatataacg gctctgccgt tgacgacctc gattttgagg tatggatgtg cggggatcgt
     6661 agccattaag gcagcctcct gtaacagtgg aacagttcca ccaccggagg tctcaatctc
     6721 gctggtggcg gactggacag ggttgagact accggcgtta caggaaaccg gcgaaccttt
     6781 cggttcccct gcccagccca ccataattct ggcgtgcaag gacgctgaca acaaaaaaga
     6841 cgctggcgcg tcatttgtcg cctgtaacaa ttccggggtc tcactcccga tagtggattt
     6901 tgccactata tgtaaagtgt gccgccggaa tttaattttg tcaacgaggt taaatataga
     6961 gtcgctatgt tggtggcgct tcgcactcaa caaaatcacg acgaaacaac cacagcggga
     7021 tgaagcattc atgcggataa ccatcacgca ggtaaataac ccgctgtgtt tcagactccc
     7081 agcgtataac gtggacgcga cgcccccttc catcacggaa ccagcgattg agtacttgca
     7141 tgtgttacct gtgagcataa ttacacctgc cagcccagcg cctggaacaa ccccattttc
     7201 gggtgatacc agcgagtacc tcgtggttct gcttcactcg tcatgcgatg aaaagcagac
     7261 atgaagggtt ccacagcaac aatagcgcga cgcgataaca atccatccgg cgtcatgaac
     7321 tcatgggtgt cggtagggat ctgatatgcg ttcaccagat tgcggcattt cgcatctgac
     7381 atacccgttt tcgccgccag ctgacgatag cctgcataac catcgcgtat ggcgcctctt
     7441 ttgatttgtt cgacagtttc ggtaacgtga ctgacttttt cttctacccg ctcaaggcgt
     7501 ttctgctgac gaacagcttc aagagccatc gcggcaacca tttcgatttc gctcattggc
     7561 ttgcggatct gttcttccag ttcgcgccag cggtctacca gacgggctgt gaattcaggg
     7621 caaagctgtg cgacaacaat gatgctgtcg cgtttgcctt gttcgccttc aaacagataa
     7681 tgctcgtgtt gaactttaaa ccctaagtta ttgattcttt cggaaacctc aatttgagga
     7741 gatcggacaa caccaccttt agctaatgtt tcaatagtgc gtttcacatt gtcatgacgc
     7801 ttacccacca gctctgcgat ctcaacgctg gtcatggatg ctttatcggt aaaaattgtt
     7861 gtattcactg tttatctcct tcgcacactc catcttctgt gtgcgctaaa cttgggtgtg
     7921 ggaaaagctc cggtagatca ggacgaaatt catacgcctg gactttccca tcgaccgcct
     7981 taaccagaga cggaacaaat tcaggagaaa tttttttctt cccattcagc cagtcgcaaa
     8041 ttgttgactg cgcgcgacca caacgcctag ccagagcaat ctggcttccc gcaatatcta
     8101 cagccttggc tataccttca tttttcatat cgacctccaa cctgattcca ttcactatag
     8161 cgattgatta aagcgattgc aatgtacggg aaagcggttt gtgtatatat cgctaaggcg
     8221 atacactaaa agggcagaaa aaggaggact tatgagcttt tcagacaggc tacagttagc
     8281 tatgactatc cgcggatact cgcagggaaa actggccaga gaggttggca tggctcaatc
     8341 gagcgtaaat aaactggtta atggtgccac aggctccagg aaagttgtgg agatagccaa
     8401 taccctgaac gtgcgccccg agtggctatc gtacggtgtt ggtcctatga ccccagaaga
     8461 aagcacatcc tgcaatccga caccacaggt atcatattcc ccgctggaaa atgataagga
     8521 tatttttcgt gtggaagtcc tggatatcgc tgccagcgcg ggaccaggaa cattccttgt
     8581 ttcagatttt gcagaaacgg tccatgctat agaattttca catgacgccg caagacgtct
     8641 gttctgtagc aggccagcaa atatcataaa gatgataaca gttgatggcg acagcatggc
     8701 tcctactctg tgtgcaggag atcaggtgtt tgtcgacgta tccgtcagaa attttgaaac
     8761 tgacggtatc tatattttta tttttgggca cactttccat atcaaacgcc tgcaaatgca
     8821 agggatgcaa cttgctgtta tatcagacaa tccggcctac aaggaatggt ttataagtga
     8881 atcagctgaa gaacatctat tcataatggg gaaagttctc attcatcagt caatccagta
     8941 taacagggtc ggataagtca acatcaccgg cagctgtatt gcgcagctgc tctactttcc
     9001 tgtatctcaa ctccctacgt atcgtctcat ttctctcata atctgttctt tgctgcccaa
     9061 aatgcatagc tatagcgatt gacataacca aacgctttgg ctattgtaaa attaaaacaa
     9121 caggaggctc atatgaagca tgcagttaag gtgatggatg aaattcagca caaatacaag
     9181 aaagcggctc gctgcttgga atgatctatt cctacatgtc agaaaacgga gcagaaagtg
     9241 agagggttta taatgcaatc agatgcctga tgcgttccct gcaaaaaaca gaagaaactg
     9301 catcaatttt tgcagaaaaa ctatgtgagg caaaagctat ggtttcgcat ggtcactttt
     9361 ttggaaaaat ccgtgcccaa cgtgaagcct gcggcttgac gaccaccgaa cttgccaaac
     9421 tgcttgatct cgatgaagag atcatcctgc aatgggagag cggagagttc gaaccgacca
     9481 tcagcatgct tatccccctg gcaaacgtca ttgggtgcga tccgctttct ttgctggatg
     9541 aaaaagatag cgcgtctgtt attcgcgtaa atgcgcctga agtctacgtg gaaagtattg
     9601 gcgcacgcat caaaagcgca cgtaaaaaac tgggcttaac ggaagctgat cttgcccgca
     9661 tgattaatac ctatagcgac cccataaacg actgggaatg cggcatctgt gaagttcctg
     9721 ctgatcagat agtgccactg gccagtgcgc ttaattgtga cctgatgtgg ttgttaacgg
     9781 gaaaatcaga agcaaaggag tagcaacaat gactggtaat atccatgata agtatgaagg
     9841 cttatgccta gcaccagatt ccttcgcaaa caatatccat gatttattat gcgcagttgt
     9901 cgtattacaa atgtcagaca acgacgcaat aaaaagaaca ggtgatgaag ttcttgaatt
     9961 tgcacgttgc tatgctgaag cagcagctga aaaagaacta tccagttaaa tagaacaagt
    10021 catctccaga tactatatta cggcttaatc gccggggatt atcacacact taatccacag
    10081 gaggttttat tatgactttt ataaaacata aggcatcaca caaaacagcc tgccttattg
    10141 cacaacacgg gaaaaattac atgcatattg cctgcttgtt tctgcgtaaa gcatacggga
    10201 gataataatg catcagaaaa cagcagaaca cgaacaaacc agagtattgc tgaccatcaa
    10261 aaacgggaaa gtaatattaa ttcgtcatgt tcatgacgat gaatttgtag gaaatctttc
    10321 aacattcctg tttattgcag aaaaggcagg atatgacgtt attgcaccag cagatgaaga
    10381 tgaggaataa atttcatgca atacgctgaa ttccaggctg aagcaacagc caatggtata
    10441 caaacaggca gtatgactat tgattatcac gacgccatac gccgtctgga tgctggagaa
    10501 ttcgatactc ctaatgtgcg aggtttacgt atccttcagt gtctcgcgca agctgacgaa
    10561 gcaggattac tgggaaaact tccggttgag atgaaggttg cccagtggcg atggttgtat
    10621 gtgacgacat tcatcaacga agaagaagac aagaacggca caattgatgt cccgaatgaa
    10681 cacggaacaa cagatcgcgc cgtaatatat aacgggaagc atggagttat gactatatac
    10741 cctagtccca ttcggtttgc cttacagcag tatattgaat ggaatttaat tcaaacatat
    10801 ggcgaagctg agggaatggg aagagcgctg tttctttatc agaaaatgct caccacttcc
    10861 cctgataaag gtttcatcct ttcagatata ggtcgagaag ggcttgaact ccttctggat
    10921 gaaatgatta acgacctgaa tactcatggt atgccagaag gccagtgaca cattaaatat
    10981 taagaagaat ataactcttc cgttttttac taaccgttta tatgaaaagc gaccgtgaat
    11041 taagcagagt aaaactgctt ttaatccttg ccacagtact gacactaaca gaaatcatta
    11101 ttctctttat tgcgctgtct gtcagttaaa aatatcggga taccacatac caatgagact
    11161 gtatttcaca atagtaattt tactggcaat tatcgcatgc atttacggat tactcgttcc
    11221 gttccttata tccatgaagg atacgagagc agttatttct ggctttgcac tggcgtttct
    11281 gaccccgccc tgcatttatg ccatttacaa gggtctttct ttcactaagg ataaaaaatg
    11341 aaaaaaatta tttttgcttt agccattgtt ctgccgacca ttggtcttgt cggttgcgat
    11401 cgcgttgagc ccggtaatgt tggcatcaaa gtaaataaac tgggcgacga taaaggcgtc
    11461 ggtgaggtgg tcggtgttgg tcgctactgg actggctgga atactgaagt ttacatcttc
    11521 cccaccttca agcaaatgaa gacctacgat gaaccgttca gctttcagat gagtgacggc
    11581 acaaccatcg gctatcacat cggtgtggcc tacaaggttg atccatccaa agttactaca
    11641 gtgtttcaaa cctaccgcaa aggcgtggat gacattaccg acactgacct gcgccagaag
    11701 atagccgacg cactcaaccg actggccagc aaaatgacca ccgataaatt tatcgacggt
    11761 ggcaagtctg aactgctgga ttcagcactt aaagatattc aggcagaaat gacacccatc
    11821 ggcattcagg taatgagcct ctcttatgtc ggtaaaccgg aatacccgcc aaccgtgatc
    11881 gacagcatta atgccaaagt cacagcgaac cagaaaaccc tgcaacgcga acaggaagta
    11941 aaacagcgcg aagcggaagc caacatgttg cgcgcggaag cagccggaca ggctgacgcc
    12001 attcgcacaa aagcccaggc cgaagccgat gctattcgtt tacgcggtga agctctgcgc
    12061 cagaaccccg gtgttatgga gctggaagcc atcaataaat ggaacggcac actgccgcaa
    12121 tacatgacca gcgatgccaa tacaccattt atccaggtta aataacttat atgcccggca
    12181 ggccgccggg ctaagggaaa agcagatgaa cacccataat gcccaaccgc aaataatgaa
    12241 ctatgacccg aatctgacgt catgcggacg catggcaaaa caaaccgttc gattaacttt
    12301 cgggttatgg gaataccgcg aaacattcga agttactgtc ggcggcaatc tgaccggact
    12361 ggatgttatc agttgcgcta ttgaaagcct gtacgcaaca ctgccttatg aagaagtcga
    12421 ggatgagcgc acagggggaa cagatatcat ggccaccatt aatattggcg aactgatatg
    12481 tcaggatgaa gacctgtccg gagagagctg gcttgctgga atgcttgtag ctgcagaaat
    12541 tatcagtatt gaacccgcta caaatatacg gctctgaagt tctcgctatt cagagaacag
    12601 gagaaaaaat gttcgatttg attaatcaag gacaactgta taccgacagc gccggttacc
    12661 cggtaaaaat tattcactgc ataaataaca tcgtgttgta cagaagaatg gatggacgaa
    12721 cacagtcggt aaaaataaac gattttaatg aactgtttga acgaatcgat caccaggaat
    12781 accgacaaat tctggcagaa acagaacaag aagctcatct gaaaaaatta cgtgccatga
    12841 aaaggaagta aagaatgaat aaagcgtttg agctatgggt acgccagtgt tacggcaatc
    12901 gctatgacct gacgcgagat gttgacggtt tctactgccg tgaagttgtg aaacgaatgt
    12961 ttgacgtgtg gtgccactgc cgtggatgaa aattttatga ggttggcatg cagacaatca
    13021 tctatcagat aacccccagc aaatggtgta cggagagagt cctcattgca tcaacagggc
    13081 taaagcctgg caccattgag cgggcaagaa gaaagtcatg gatgcaggga aaagaatacc
    13141 gccattacgc tgtagaaggt gatccagggc attacagtga atgcctgtac aacatcgaag
    13201 aaattatgcg atggatcgaa aaccagaaac aaccaggtgt caaaaatgca agttccggtt
    13261 aacctgttaa tgctcctgga cgtctgggag gttcaatgag taacacatca tacccgacag
    13321 gcgttgaaaa ccatggcgga tcactccgca tatggtttca ctataacggc aaacgtgtca
    13381 gagaaaacct cggtgttcct gacacagcca aaaaccggaa gattgcaggc gaactcagga
    13441 cttcagtttg ttttgcaatc agaatgggga atttcgacta cgctgcgcag tttcctgagt
    13501 ccccaaatct gaaacacttt ggtctgggaa aaaaggagat aaccattaag gcgctttcag
    13561 aaaaatggct ggatctgaaa aaaatcgaaa taggaggtaa cgcattcaat cggtatcaat
    13621 ctgtggtgag aaacatgttg cctcgtatag gggaaagccg actggcttca tctgtaacaa
    13681 aagaagattt actcttcatc aggaaagatt tattgaccgg gtatcacaat ctctcaaacg
    13741 gcaaaacaac acctgttaag gggagatctg tagttacagt taattactac atgacgacaa
    13801 ttgcaggtat gtttcaattt gcagctgaca acggctacac ggaatcaaac ccgtttaacg
    13861 gcctgacacc attaaaaagg tccagaaccg agccagatcc gctcacgcat gacgaattca
    13921 ttcgttttat tgatgcttgc caccatcaac aaacgaaaaa cctgtggtct ttagcagtat
    13981 acacaggcat ccgtcacggt gagctaatat ctctcgcctg ggaggatatt gatttaaaag
    14041 ctaaaacaat gactatccgt cgtaattata caaaactcgg ggagtttact ctaccaaaaa
    14101 ccgaagcggg aacagatcgt gttattcatc ttgttcaacc agccattgaa accctgaaaa
    14161 gtcaggctga aatgacaaga tttggcactc aataccagat tgacgtcaaa ctgcgggagt
    14221 tcggacgtac cgtaagccat gaatgcacgt ttgtctttaa tccgcaactg gtgaaaaagt
    14281 gccagcaagc cggttaccac tacaaagcgg actccatcag ggattcatgg gcatcagccc
    14341 tgagacgggc cggactacgg cacaggaaag cctatcagtc aaggcatact tatgcctgct
    14401 gggcactggc cgcaggagcg aatccaagct ttatcgcaaa ccagatgggg cacgcaaacg
    14461 ctcagatggt attcaatgtt tacggagcat ggatgaaaga taacaatatc gggcagatag
    14521 aactgctcaa taaggagtta gctgaaagtg tcccatacat gccccacaga gcaagacgct
    14581 gaaataattt ctttcagtat cattgagtta acccatggtt cccgccatgt taactgcgtg
    14641 gagggcaaca ccacgcttta cgccctgccg aaacccgagg ttgtcctgcg ctggcgtgag
    14701 cagaccacag atgacttccg cttctgtttt aagtttccgg cgaccatttc gcatcaggca
    14761 gcattacggc attgcgatga tttagtgact gaatttttga cccgcatgtc accgttggct
    14821 ccgcgcattg ggcaatactg gctgcaactg cctgccacat tcggcccacg ggagctgcct
    14881 gcgctttggc attttctcga ttctctccct ggcgaattta attatggtgt ggaagtccgc
    14941 catccacagt ttttcgccaa aggggaagag gaacaaacgc ttaatcgcgg tttacatcag
    15001 cgcggcgtta atcgggtgat tttagacagc cgcccggttc atgcagcacg tccacacagt
    15061 gaagctattc gcgacgctca acgaaaaaaa cctaaagttc cggtacatgc tgtactgacg
    15121 gcgacaaatc cactgatccg ttttatcggt agtgatgata tgacgcaaaa ccgggaatta
    15181 tttcaggtct ggttacaaaa attagcgcag tggcatcaga ccactacgcc ttatcttttt
    15241 ttacatacgc cagatattgc ccaggccccg gaactggtac ataccctgtg ggaagactta
    15301 cgtaaaacgc ttccagagat cggagcagtt ccggctattc cacagcaatc ttctcttttc
    15361 tgaatttgcc acctatcata gacaggtgcc atcggccatt ttaaagggag tttgtatggt
    15421 aagcgcgctg tatgccgttt taagtgcgtt gttattaatg aagttctctt ttgatgtcgt
    15481 tcgcctgcga atgcagtacc gcgttgccta tggtgacggt ggttttagcg aactgcaaag
    15541 cgctattcgc attcatggta acgcggtgga atatattccc atcgcgattg tgttgatgct
    15601 gtttatggaa atgaatggcg cagaaacctg gatggtgcat atttgcggca tcgttttgct
    15661 tgctggtcgt ctgatgcatt attacggttt tcatcaccgt ctgtttcgct ggcgacgttc
    15721 cggcatgagc gccacctggt gtgcgctgtt gctgatggtg ctggcgaatc tttggtatat
    15781 gccctgggag ttggttttct ccctgcgtta gcgcacaata cgccactttc tttttcccgg
    15841 atttttacgt tatgtctcac cgcgacacgc tattttctgc ccctatcgcc agactgggcg
    15901 actggacctt tgatgaacgg gtagctgaag tcttcccgga tatgatccag cgttccgttc
    15961 ccggctattc caatattatt tccatgattg gtatgttagc cgagcgcttc gttcaacctg
    16021 gtacgcaggt ttacgatctg ggttgttctc tgggcgcggc gacgctctcg gtgcgtcgca
    16081 acattcatca tgataattgc aaaattattg ccattgacaa ctccccggcg atgattgaac
    16141 gctgccgtcg tcatattgac gcctataaag cccctacgcc agtagacgtc attgaaggtg
    16201 atattcgcga tatcgccatt gaaaacgcat cgatggtggt gctgaatttt accctgcaat
    16261 tcctggaacc ttccgagcgc caggcgttac tggataaaat ttatcaaggg ctgaacccgg
    16321 gcggcgcgct ggtgctttcg gaaaaattca gtttcgaaga tgccaaagtt ggtgaactgc
    16381 tgttcaacat gcaccacgac tttaaacgtg ccaacggtta cagcgaactg gagatcagcc
    16441 agaagcgcag catgctggaa aacgtgatgc tgaccgattc cgtggaaacc cataaagcac
    16501 gcctgcataa agccggtttt gagcatagcg agctgtggtt ccagtgcttt aactttggtt
    16561 cactggtggc attaaaagca gaggacgctg catgatcgac tttggtaact tttattctct
    16621 gattgccaaa aatcatcttt cacactggct cgaaacgctg cccgcgcaga ttgctaactg
    16681 gcagcgcgag cagcagcacg ggttgtttaa gcagtggtca aatgcggtgg agtttctacc
    16741 tgaaattaaa ccgtatcgtc ttgatttatt gcatagcgtt acggcagaaa gcgaagagcc
    16801 actgagcgca gggcaaatta aacgcattga aacgctgatg cgcaacctga tgccgtggcg
    16861 caaagggccg ttctcactgt atggcgtcaa catcgatacc gaatggcgtt ccgactggaa
    16921 atgggatcgt gttctgcccc atctttctga tttaaccgga cgcaccattc tggatgtcgg
    16981 ctgcggcagc ggttatcaca tgtggcgcat gattggcgca ggcgcgcacc tcgcggtggg
    17041 gatcgaccct acgcaactgt ttctgtgcca gtttgaagcg gtgcgtaaac tactgggtaa
    17101 tgatcagcgc gcgcatttgt taccgttagg tattgaacaa cttccggcac tgaaagcctt
    17161 tgataccgtc ttttcgatgg gcgtgcttta tcatcgtcgt tcaccgctgg agcatctctg
    17221 gcagttgaaa gatcaactgg tcaatgaagg tgaactggtg cttgaaacgc tggttattga
    17281 tggcgatgag aacacggtgc tggtgccggg cgatcgttac gcgcaaatgc gtaatgtcta
    17341 tttcatccct tccgcgctgg cgctgaaaaa ctggctgaag aagtgtggtt ttgtagatat
    17401 ccgcattgcg gatgtgagcg ttaccactac agaagagcag cgacgcactg aatggatggt
    17461 taccgagtct ctgtccgatt tcctcgaccc acatgatccg agtaagacag ttgaaggtta
    17521 tcctgcgcct aaacgcgcgg tgctgattgc gcgcaagccg taaaggtctg gtattactgc
    17581 cggatgcggc gtgaacgcct tatccagcct acaaaacctt gctaattcaa tatattgcag
    17641 ggactatgta ggcctgataa gcatagcgca tcaggcagct ttatgtttgc ataacctcag
    17701 cgcccgtttc cgggcgcttt tcacgtctta cgcctgaaca gctggctgat caaacgccgt
    17761 taacttcggt gcgttacccg tatatttttc gatatacacc agcgcagagt taccggcaca
    17821 ggcatttgcc agctgcgagc tggggatatc cgccgttaac acgttcgcac tgccgttttt
    17881 acacaagcca ttttccagat ctggccatgc accttcatgc acgcatacca cgcctttttt
    17941 gatcccgtca gtcaccaccg cacctgtcag aatctgacca cgtttgttcc agacacgcac
    18001 cagatcatca ttcgcaatac caaagcgagc agcatcttcg gtgtgaatag taattggttc
    18061 acgatcggca accgcatatt ttttacgcag ttccgcatag ttaagctgac tgtgtaaacg
    18121 gtgtgccgga tgtgcggtca gaagctgcaa ctgcttctcg tcggcggtac ccttccactc
    18181 atcaggcgca agccaggtcg ggtgtgccgg gcaatcctta tagccaaatt tctccagcgt
    18241 ttttgagtag atttcaattt tgccacttgg tgtacccagc gcatttttca ccggatctgc
    18301 gcggaaatcg ccatagcgaa cgaactgctc attcttctcg ctgcggcgca tttcaagcaa
    18361 tttattttgc tgccagaagg cattaaacat cggcatggtg acgcgttgcg cacgcgcacc
    18421 tttctgagcg gcatcataga aaaatttcag ccacgccatt tcatctctac cttcggtata
    18481 gatctctttt ccgcctggtt tcagtaattc cgcaaggtcg gcaaacacgt caaaatcgtt
    18541 gcgtgcttca aattgcggag cgacaacctg cttcatcggc acaatatgtt ggttactgta
    18601 atcgccggtc atcgtcagat cattgcctcg aacgatgtgg tgatcggtaa tacgatatcc
    18661 gcgtgtttag ctgccgccgt ccagtagcac tcagaaacga cgatcatctc cggtttctgc
    18721 caggctttaa tcagacgatt agtatcctgg tgatgggtaa agttaccgcc acccgcccac
    18781 cagatcattt tgatattcgg atacgtttgt tctttaccgt tatgctgata tttgccgcca
    18841 ggattttcca gcgcatcgac gatacgggca acaggaatcg ccgtcattcc gccatcatca
    18901 gcggcctcgc tggcctgtcc ggcaatcgcc gcagacattt ccggcagcac gccgccaaca
    18961 cgcgtcgggt tgccaccgtt ggagtaatga taagagaaac caaaaccgcc gcccggtgta
    19021 ccaatctgcc ctaacatcgc cgccagcgtc accagcatcc agtgtttctg ttcaccatat
    19081 tgctggcgct gaattcccca gcctgccatc agcattgtgc gattcgccgc catcagttca
    19141 gcgagtttga ctatctgcgc ttccggtacg ccagtaattt ccgccgccca ggccgcgctc
    19201 ttcggagtat tatcgctctt acccgtcaga tactcttcaa actgcggata cccggtggtg
    19261 tatttttcaa ggaacacttt atcgtgtttg ccttgtgtca tcagggtatg cgcaatccct
    19321 aacatcagtg ccacgtcggt gcccatattc ggcgcgatcc aggtggcatt atcgccaaag
    19381 aactcgatgg tttcggagcg gataggatca atggcaatca ctggtttgcc agattttttc
    19441 agctgatgga agtattccag cccttgctca tcggtactgc tccaggcaat ttttaaggta
    19501 ttcagcgggt tcattcccca cagcaccaca acctggctgt tttccagaat cagcggccag
    19561 gaggtctgct gttcatacac ctctacagaa ccgaccacat gcggcatgat cacctgtgct
    19621 gccccggttg aataatcgcc gctatgcccg gaataaccgc ccgccaggtt catataacgt
    19681 tgcagtaagg tttgcgcttt atgcaacacg ccagaagagc gccagccgta agatccggca
    19741 aaaatggccg atggtccgtt agctttacga atacgctcat gttgctcatg aatcagtttt
    19801 aatgcttgct cccagctcac ctgtacatag gtatcttcgc ctcgaccttt cgccggttgc
    19861 agtggattat cgagatagct ttttctcacc atcggatgct gaatacgcgc cgtggtgtgt
    19921 acctgatccg ccgccgtaga ctgtaaggag ttcggtatgg ttttcgccaa cgcgcctgtc
    19981 gaagaaacaa tcttgccgtc cttcacttct acgttcatcg ctccccaacg tcccgcggtg
    20041 aggattttac cgcccttctc ttctgcccat gcgggtagcg gtgctgccga tgtcaccacc
    20101 agcgctccag cggcaatacc gctgtgttta ataaattcac gtcttgttaa tgtcataact
    20161 tcctccctga tcaacgagga tcactgtttc tcggtaatat ctttggcgtt gtactggaaa
    20221 taccgcgtta aaatgtccag ttcgttttcg ctcatgctgg ttcgtgcccc cattcctttg
    20281 gcaatggaag gccacgcatt gacggtgtaa tggtcggcgg caataggggc atgacaacca
    20341 gcgcaatagg tatcgtcaag tttttcagcg tattgccata gcggtttacg gtccgctaat
    20401 gcgggatcgg taagcgcacc ctgtaaagac gcctgacgcc attgattgcc gtattcgtca
    20461 gcctgccatt ccccgtttac cgtgagcgcc ttgatacctt cttcacttaa tgtggctagc
    20521 gccagccgtt gacctaccgc caggtagagc gtgttttcac tgccctgcat ttgataaccc
    20581 tgcaacagaa cgatcggctg tttgccactg gcatcaacga cggtgagatc ggtaccagga
    20641 ttcacggtag ccagctcgcc tatgcgagaa gttttgaaag gataaatatg tgcgccatta
    20701 gtcactgaag tagcggcctg actttccagc tcatgcgccg cgttgtcatc catttttatt
    20761 tctggcggaa aatgggcaat gcctttatga caatcgatac aggtttcgcc gcccttttgt
    20821 gctttgttat gcattttctg cgcagattca ctttgcgagg caatatccat ggcatcaaaa
    20881 gaatggcaac tacggcacgt tgcagagtca gttgctttta attctttcca tactgtttcg
    20941 gccatttcct ggcgatgagc ttcgaactta tcgtcactgt ctattttgcc gctaacaaat
    21001 tcatgataaa tatctttaga tgcttttaat ttagcgaata aataatccat ccctgacttt
    21061 ggaatatggc aatcggcaca ttccgcacgt atccctttct ggttcgaaaa gtggacagtt
    21121 ccctgatatt cctcaaaagg tttactcatc gagtggcagg aaaggcaaaa tgctgtatcc
    21181 gacgttttat gtaaggcttt ttgcgccagc aagaacccgc cgccaccaac cacaaccgct
    21241 atcagcaaaa acaataaccc aatgcgtttt ttccctcgca tagattcctc taattaaatt
    21301 ccagaacacc gaaatcattg tataaataaa tacacaacga attgactact tgcttaattc
    21361 ggcacaacac tgcct
//
LOCUS       DAZFKF010000086        20761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFKF010000086 DAZFKF010000000
VERSION     DAZFKF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.086"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            116..799
                     /locus_tag="ACHKA1_003758"
     CDS             116..799
                     /locus_tag="ACHKA1_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO5681351.1"
                     /translation="MNLKKIFFSAVTVSVLCTLTGCDYIEEGKPESSLLKQQEEHNNK
                     IELIEKQQAQLKSQLETIQKQQTGIITSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSIPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            843..4059
                     /locus_tag="ACHKA1_003759"
                     /pseudo
     CDS             843..4059
                     /locus_tag="ACHKA1_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            4486..6141
                     /gene="lldP"
                     /locus_tag="ACHKA1_003760"
     CDS             4486..6141
                     /gene="lldP"
                     /locus_tag="ACHKA1_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO5681352.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSVVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6141..6917
                     /gene="lldR"
                     /locus_tag="ACHKA1_003761"
     CDS             6141..6917
                     /gene="lldR"
                     /locus_tag="ACHKA1_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO5681353.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            6914..8104
                     /gene="lldD"
                     /locus_tag="ACHKA1_003762"
     CDS             6914..8104
                     /gene="lldD"
                     /locus_tag="ACHKA1_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO5681354.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            8259..8732
                     /gene="trmL"
                     /locus_tag="ACHKA1_003763"
     CDS             8259..8732
                     /gene="trmL"
                     /locus_tag="ACHKA1_003763"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO5681355.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYSAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(8785..9606)
                     /gene="cysE"
                     /locus_tag="ACHKA1_003764"
     CDS             complement(8785..9606)
                     /gene="cysE"
                     /locus_tag="ACHKA1_003764"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO5681356.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKTSGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(9686..10705)
                     /gene="gpsA"
                     /locus_tag="ACHKA1_003765"
     CDS             complement(9686..10705)
                     /gene="gpsA"
                     /locus_tag="ACHKA1_003765"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO5681357.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(10705..11172)
                     /gene="secB"
                     /locus_tag="ACHKA1_003766"
     CDS             complement(10705..11172)
                     /gene="secB"
                     /locus_tag="ACHKA1_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO5681358.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11235..11486)
                     /gene="grxC"
                     /locus_tag="ACHKA1_003767"
     CDS             complement(11235..11486)
                     /gene="grxC"
                     /locus_tag="ACHKA1_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO5681359.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(11627..12058)
                     /gene="yibN"
                     /locus_tag="ACHKA1_003768"
     CDS             complement(11627..12058)
                     /gene="yibN"
                     /locus_tag="ACHKA1_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO5681360.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12303..13847
                     /gene="gpmM"
                     /locus_tag="ACHKA1_003769"
                     /gene_synonym="pgmI"
     CDS             12303..13847
                     /gene="gpmM"
                     /locus_tag="ACHKA1_003769"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO5681361.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKNRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVDEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            13881..15140
                     /gene="envC"
                     /locus_tag="ACHKA1_003770"
     CDS             13881..15140
                     /gene="envC"
                     /locus_tag="ACHKA1_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO5681362.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15144..16103
                     /gene="yibQ"
                     /locus_tag="ACHKA1_003771"
     CDS             15144..16103
                     /gene="yibQ"
                     /locus_tag="ACHKA1_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO5681363.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPNAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(16090..17124)
                     /gene="waaH"
                     /locus_tag="ACHKA1_003772"
     CDS             complement(16090..17124)
                     /gene="waaH"
                     /locus_tag="ACHKA1_003772"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO5681364.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNHGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(17363..18388)
                     /gene="tdh"
                     /locus_tag="ACHKA1_003773"
     CDS             complement(17363..18388)
                     /gene="tdh"
                     /locus_tag="ACHKA1_003773"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO5681365.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(18398..19594)
                     /gene="kbl"
                     /locus_tag="ACHKA1_003774"
     CDS             complement(18398..19594)
                     /gene="kbl"
                     /locus_tag="ACHKA1_003774"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO5681366.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(19869..20726)
                     /gene="yibB"
                     /locus_tag="ACHKA1_003775"
     CDS             complement(19869..20726)
                     /gene="yibB"
                     /locus_tag="ACHKA1_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO5681367.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     VLENEMIIFTSPDLKPRVEAIRNGKPTTVIAIDIKKKFRHIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQYKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSGLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
BASE COUNT         5230 a         5307 c         5310 g         4914 t
ORIGIN      
        1 aatatttaat cgacacccat cactgtcatt aatgtaaagc aacatagatc accaataata
       61 caatggacaa ctctaccggc attgatcgct ggtcggaggc aataaggatt tttacatgaa
      121 cttgaagaag atttttttca gtgctgtcac tgtttcagtt ctctgcacat taacgggatg
      181 tgactacatt gaagaaggca agccagaaag tagtttactg aaacaacaag aagaacataa
      241 caataaaatt gagctaattg aaaaacaaca agcacagcta aagagtcaac tggaaactat
      301 tcaaaaacaa caaactggca taataaccag tacaaagact ttaactcacg taattaaatc
      361 cgttaaagat caacaaaaca cttttatttt taccgagttt aatccggcaa aaacgaagta
      421 tttcatcctg aacaatggtt cggtggcttt agcgggccga gtgttatcca ttgacgcaac
      481 agagaatggc agtgttattc atatttcact ggtcaactta ttaagtatac caatctcaaa
      541 cattggtttt aatgcgacat ggggtggtga aaaacctgtc gatgccaaag agtttgcccg
      601 gtggcaacaa ttacttttca acacatcaat gaaatccaca ttgaaattat taccaggtca
      661 atggcaagac attaatttga cactgaaggg tgtatcgccg aataacctgg gatatctgaa
      721 gttagccatc aatatggaga atattcagtt tgacaacctt ccctctgctg aaaatcggca
      781 gaaaagaagc aaaaaataaa tgcagcttct gcgcttatat caaggaatag agaacatcat
      841 taatgaacaa aatatttaaa gttatctgga accctgcgac agggaattat actgttacca
      901 gcgaaacggc aaaaagccgt ggcaagaaat ccgggcgcag taagctgtta atttctgccg
      961 tggttgcggg tgggttgttg tcgtcgtttg aggcactggc aacaggtaat gaacctggta
     1021 cacctgttgg cacaggatct ggctgggttg ctataggcca ggaatcacag gcaaatgctt
     1081 ttaccgatgg taatggtgcc agtactgcag tgggttataa agctttcgct gaaggaacat
     1141 ggagttccgc tattggcgcc atgacccatg caattggtcc ggcttcaatg gcattcgggg
     1201 ttaatgccgt ctcaaccggt caacgaagta tcgctatggg cgcatcgtca agttcagtgg
     1261 tcaatattcg atggcacttg ggcgatatgc gaattcgggt ggtgattatt cgatcgcgca
     1321 aggggataaa tccaatgctg ctggtctgaa tgccattgca gtgggaagaa gcagtagtgc
     1381 ggcaggcgag aaagccactg cgctgggcaa cgcagctaaa gctactgaga ttatgagtat
     1441 tgctcttggc gacagcgcca atgcgtcaaa agcgtattca atggcgctgg tagcaagtag
     1501 cgtcgcatct gaagaaaacg caattgccct ggggcgtagc agttagctag cggtactgac
     1561 agcctcgcat taggcagaca aacacttgcc agtacagcga acgctattgc gataggtgct
     1621 gagagcaaag ccgctgaaaa tgcgacggcc gttggtacca aagcggaggc caatggactt
     1681 aatagtatcg cgttgggctc tgggggtatt gcagatgccg ataacacgat tgctctgggc
     1741 aatcaaagcc tggcagtagc tgctggtgca attgccatcg gtcaggggaa taaagctgac
     1801 ggcgcaaatg ctatcgcgct gggtaatggt agtgttacag gtggcgcaaa tgctattgct
     1861 cttggacaag gtagttatgc cggtttagaa aatggcattg caattggtgg tcaagccagt
     1921 gctcaaggga caaattccgt tgctctgggt gcgggttctg tagcgactga agataattcc
     1981 gtctctgtgg gtaacacaac agatcagcga aaaattgtca atatggcagc aggtgatatc
     2041 agcactagca gtactgatgc cataaatgga tcacagcttt atgctatcag taagtcagta
     2101 gcggaccgtc ttggtggggg ggctaccgtc aatgcgcaag gtgtcgttac tgccccgaat
     2161 tacagactga aaaatggtat ttatggcaat gttggcgatg ctttagcgga tctaaacaat
     2221 aacacgatac aatgggactc cgttaaaaag ggatatagtg cggcacatgg tacagatgcc
     2281 accagcacca tcaccaacgt taaagccggc actatttctg ctaccagtac ggatgcggtt
     2341 aacggttctc agttgaatgc gacgaatatg atgattgagc agaacagcca aatcatcaat
     2401 cagcttgctg gtaacaccga cgcaacctat atcgaagaaa atggcgctgg tatcaactac
     2461 gttcgtacta acgacaacgg tttagccttt aacgatgcaa gcgcctctgg tgtcggcgct
     2521 acagctgtag gttataacgc tgtcgcgtct ggtgccagca gcgtagccat tggtcagaac
     2581 agcaatagca ccgttgatac cggtattgcg ctgggtagca gctccgtttc cagccgtgtg
     2641 attgccaaag gttctcgtga cactagcgta acggaaaatg gcgtggtgat tggttatgac
     2701 accactgacg gcgaactgct aggtgcattg tcaattggta atgacggtaa ataccgccaa
     2761 atcatcaacg tagctgatgg ttccgaagcc catgacgccg ttacggttcg tcaattgcag
     2821 aatgcgattg gtgcggtcgc aaccacgcca accaagtact ttcacgccaa ctcaacggca
     2881 gaagactcac tggccgttgg tgaagactcg ctggcaatgg gtgcgaaaac tgtcgttaat
     2941 ggtaatgcag gtattggtat cggtttgaac actctggttc tggctgatgc gatcaacggc
     3001 attgctatcg gcagtaacgc acgcgcaaac catgcaaaca gtattgcaat gggtaacggt
     3061 tctcagacca cccgtggtgc acagactggc tacaccgcct acaacatgga cgcaccgcag
     3121 aactctgtag gtgagttttc tgttggtagt gaagacggtc aacgtcagat caccaacgtc
     3181 gcagctggtt cagcggatac cgatgcggtc aacgtaggtc agttgaaagt aacggatgcg
     3241 caggtttccc agaataccca gagcattact aacctgaaca atcaggtcac taatctggat
     3301 actcgcgtta ctaatatcga aaacggtatt ggcgatatcg tcaccaccgg tagcatcaag
     3361 tacttcaaga ccaataccga tggtgtagat gccagcgcgc agggtaaaga tagcgtcgcg
     3421 attggttctg gttccattgc tgccgcggat aacagcgtcg cactgggtac agggtctgtg
     3481 gcaaccgaag aaaatacgat ctctgtaggc tcatctacta atcaacgtcg tatcaccaac
     3541 gtagccgcag gtaaaaacga caccgatgct gttaacgtgg cacagttgaa gtcttctgag
     3601 gcgggcggtg tacgttacga caccaacgct gatggttctg ttgactatag caatatcacc
     3661 ctcggtgggg gcaacggcgg tacgactcgt atcagcaacg tctccgctgg cgtcaacaac
     3721 aacgacgcgg tgaactacgc gcagttgaag caaagcgtgc aggaaacgaa gcaatacacc
     3781 gatcagcgga tggttgagat ggataacaaa ctgtctaaaa ctgaaagcaa gttgagcggt
     3841 ggtatcgctt ctgcaatggc aatgaccggt ctgccgcagg cttacactcc aggtgccagc
     3901 atggcctcta ttggtggcgg tacttacaac ggtgaatcgg ctgttgcttt aggtgtatcg
     3961 atggtgagcg ccaatggtcg ttgggtctac aaattacaag gtagtaccaa tagccagggt
     4021 gaatactccg ccgcactcgg tgccggtatt cagtggtaat gatccattaa gttagtgtga
     4081 ctaagggcaa acgccaaatt gcctgatgcg ctacgcttat caggcctaca gggtgattct
     4141 gcacattcct ataggccggg taaggtgttc acgccgcatc tggcaagata aggcgctccg
     4201 ggtcaacaat ctaagggcaa ttctctgatg aggattgccc ttttctttac cagacatctc
     4261 cccccacaag aattggccct accaattctt cgcttatctg acctctggtt cacaattccc
     4321 caattaaaac tcacatcaat gttgccaata cataacattt agttaactat tcattgtcat
     4381 tatccctaca caacacaatt ggcagtgcca cttttacaca acgtgtgaca aggagatgag
     4441 caacagactc attacacgat gtgcgtggac tccaggagac ctgcaatgaa tctctggcaa
     4501 caaaactacg atcccgccgg gaatatctgg ctttccagtc tgatagcatc gcttcccatc
     4561 ctgtttttct tctttgcgct gattaagctc aaactgaaag gatacgtcgc cgcctcatgg
     4621 acggtggcaa tcgcccttgc cgtggctttg ctgttctata aaatgccggt cgctaacgcg
     4681 ctggcctcgg tggtttatgg cttcttctac gggttgtggc ccatcgcgtg gatcattatt
     4741 gcagcagtgt tcgtctataa gatctcggtg aaaaccgggc agtttgacat cattcgctcg
     4801 tctattcttt cgataacccc agaccagcgc ctgcaaatgc tgatcgtcgg tttctgtttc
     4861 ggcgctttcc ttgaaggagc cgcaggcttt ggcgcaccgg tagcaattac cgccgcattg
     4921 ctggtcggtc tgggctttaa accactgtac gccgccgggc tgtgcctgat tgttaacacc
     4981 gcgccagtgg catttggcgc gatgggcatt ccaatcctgg ttgccggaca ggtaacaggt
     5041 atcgacagct ttgagattgg tcagatggtg gggcggcagc taccgtttat gaccattatc
     5101 gtgctgttct ggatcatggc gattatggac ggctggcgcg gtatcaaaga gacgtggcct
     5161 gcggtcgtgg ttgcgggcgg ctcgtttgcc atcgctcagt accttagctc taacttcatt
     5221 gggccggagc tgccggacat tatctcttcg ctggtatcac tgctctgcct gacgctgttc
     5281 ctcaaacgct ggcagccagt gcgcgtattc cgttttggtg atttgggggc gtcacaggtt
     5341 gatatgacgc tggcccacac cggttacact gcgggtcagg tgttacgtgc ctggacaccg
     5401 ttcctgttcc tgaccgctac cgtaacactg tggagtatcc cgccgtttaa agccctgttc
     5461 gcatcgggtg gcgcgctgta tgagtgggtg atcaatattc cggtgccgta cctcgataaa
     5521 ctggttgccc gtatgccgcc agtggtcagc gaggctacag cctatgccgc cgtgtttaag
     5581 ttcgactggt tctctgccac cggtaccgcc attctgtttg ctgcgctgct ctcggttgtc
     5641 tggctgaaga tgaaaccgtc tgacgctatc agcaccttcg gcagcacgct gaaagaactg
     5701 gctctgccca tctactccat cggtatggtg ctggcattcg cctttatttc gaactattcc
     5761 ggactgtcat caacactggc gctggcactg gcgcacaccg gtcatgcatt caccttcttc
     5821 tcgccgttcc tcggctggct gggggtattc ctgaccgggt cggatacctc atctaacgcc
     5881 ctgttcgccg cgctgcaagc caccgcagca caacaaattg gcgtctctga tctgttgctg
     5941 gttgccgcca ataccaccgg tggcgtcacc ggtaagatga tctccccgca atctatcgct
     6001 atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg ctttactgtc
     6061 aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca ggcttatgtc
     6121 ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac gaggttgccg
     6181 atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag ttgcccgctg
     6241 aacgtcaact ggcgatgcaa ctcggcgtat cacgtaattc actgcgcgag gcgctggcaa
     6301 aactggtgag cgaaggcgtg ctgctcagtc gacgcggcgg cgggacgttt attcgctggc
     6361 gtcatgacac atggtcggag caaaacatcg tccagccgct aaaaacgctg atggccgatg
     6421 atccggatta cagtttcgat attctggaag cacgctacgc cattgaagcc agcaccgcat
     6481 ggcatgcggc aatgcgcgcc acacctggcg acaaagaaaa gattcagctt tgctttgaag
     6541 caacgctaag tgaagacccg gatatcgcct cacaagcgga cgttcgtttt catctggcga
     6601 ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc ttcgatgtcc
     6661 tgcaatcctc agtgaagcat agccgtcagc ggatgtatct ggtgccaccg gttttttcac
     6721 aactgaccga acaacatcag gctgtcattg acgccatttt tgctggtgat gccgacgggg
     6781 cgcgtaaagc aatgatggcg caccttagtt ttgttcacac cacaatgaaa cgattcgatg
     6841 aagatcaggc tcgccacgca cggattaccc gcctgcccgg tgagcataat gagcattcga
     6901 gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag cgcaacgcat
     6961 tctgccgccg ttcctgttcc actatatgga tgggggtgca tattctgaat acacgctgcg
     7021 ccgcaacgtg gaagatttgt cagaagtggc gctgcgccag cgtattctga aaaacatgtc
     7081 cgacttaagc ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg tggcgctagg
     7141 tccggtaggt ttgtgtggca tgtatgcgcg acgcggcgaa gttcaggcag ccaaagcggc
     7201 ggatgcgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga ttgaagaagt
     7261 cgcgccagcc atcaagcgcc caatgtggtt ccagctttat gtactgcgcg atcgcggctt
     7321 tatgcgtaac gcgctggagc gagcaaaagc agcgggttgt tcgacgctgg ttttcaccgt
     7381 ggatatgccg acaccgggag cgcgttatcg tgatgcgcat tctggaatga gcggcccgaa
     7441 cgcggcaatg cgccgctatt tgcaggcggt gacgcatccg caatgggcgt gggatgtggg
     7501 cctgaacggt cgtccgcatg atttaggaaa tatttcggct tacctcggca aaccgaccgg
     7561 actggaagat tacatcggct ggctggggaa taacttcgat ccgtccatct catggaaaga
     7621 ccttgagtgg atccgcgatt tctgggatgg cccgatggtg atcaaaggga tcctcgatcc
     7681 ggaagatgcg cgcgatgcag tacgttttgg tgctgatggg attgtagttt ctaaccacgg
     7741 tggccgccag ctggacggtg tactctcttc cgctcgtgcg ttgcccgcta ttgcagatgc
     7801 ggtgaaaggt gatatagcca ttctggcgga tagcggaatt cgtaacgggc ttgatgtcgt
     7861 gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc tgtatgcgct
     7921 ggcaacagcg ggccaggcgg gtgtagctaa cctgctaaat ctgatcgaaa aagagatgaa
     7981 agtggcgatg acgctgactg gcgcgaaatc gatcagcgaa attacgcaag attcgctggt
     8041 gcaggggctg ggtaaagagt tgcctgcggc actggctccc atggcgaaag ggaatgcggc
     8101 atagtcgttt gcgccccctc cccctaaccc tctccctcag ggagctgtct cttgatcaga
     8161 tctcctgatc aagagacagg gggagggccg gggtgagggg aaaaggccgc acggttcacc
     8221 aattttctgc tatcctgccc ccgcactaag ggggcagtat gctaaacatc gtactttacg
     8281 aaccagaaat tccgccaaat actggcaaca tcatccgtct ttgcgctaat accggctttc
     8341 gtctgcatat catcgaaccg atgggatttg cctgggacga taagcgcctg cgccgcgcgg
     8401 ggctggacta tcacgagttt accgccgtta cgcgtcatca tgactacagc gcgttcctcg
     8461 aagcagaaaa tccccagcgc ctgtttgccc tcaccacgaa aggtacgcct gctcacagcg
     8521 ccgtaagcta tcaggatggc gattatctga tgttcggccc ggaaacacgc ggcctgccag
     8581 cgagcattct tgatgccctg cccgctgaac aaaaaattcg cattccgatg gtgccagaca
     8641 gccgcagcat gaacctgtcc aatgcggtgt cggtagtggt gtatgaagcc tggcggcagc
     8701 tgggatatcc gggagcggtg ttgagagatt agttacgcca tgccggatag atgattacat
     8761 cgcatccggc acgatcacag gacatcagat cccatcccca tactcaaatg tatggttaat
     8821 accgttgaaa tgctggtcca tatccattga tggcttatcg ctgtctggtt taccgacaat
     8881 gcgtgccgga acgccagcgg cggtggtatg cggcggcacc ggttgcagca ccacggaacc
     8941 tgcgccaatc ttcgcgccgc gaccaacttc aatattgccg agaatcttcg cgcccgcgcc
     9001 aatcatcacg ccttcacgaa ttttcgggtg acggtcgccg ctagttttcc cagtaccgcc
     9061 aagggtgacg gattgcagaa tcgacacgtc gttttcaatc accgccgttt cacccacaac
     9121 gatacccgtg gcgtggtcaa gcatgatacc gcgaccaatt tttgctgccg ggtgaatatc
     9181 aacctggaac gtcacagaaa cctggttttg cagaaagatt gccagtgcgc gacgcccctg
     9241 attccacaac cagtgaccga tgcgataggc ctgcaaggca tgaaaaccct tcaggtataa
     9301 caacggggtt gagtatttat cgactgccgg gtcgcgggta cgcaccgcct gaatatcaca
     9361 ggccgcagag gcgatcattt ccgggtcagc ggcgtaggct tcttccacca cttcacggat
     9421 agcaatagca ggcataattg gcgatgacag cttgttcgcc agcatgtagc tcagtgcact
     9481 gccaaggttt tcgtgcttga gtagcgtcgc gtggtaaaaa ctggccagca ttggctcaca
     9541 gtccgccagc gttctggctt cggctttaat attgttccag acaatttcca gttcttcaca
     9601 cgacattgct tactccacac gatgagataa tgaccggccc gttctgcgcg ggccgggtca
     9661 tagcggtaac aaaggttccc tggggttagt ggctgctgcg ctcgtccttg cgtgcacgac
     9721 ctaataacgt caatgctgcc tcgcgcgcgt tttttccgca atataatact tgataaattt
     9781 cctcggttat tggcatttca acgccgaagc gatgcgccag ttcgcggact tctttcgtat
     9841 tgcggtagcc ttccaccacc tgaccaatct tctcctgcgc gctttgtaca tccatgccct
     9901 gaccgagcat catgccaaaa cggcggttac gcgactggtt gtcggtacag gtaagcacca
     9961 gatcgcccag ccccgccatg cccataaagg tggcaggatc ggcacccagc gccgcgccaa
    10021 gacgcgacat ttcagccagg ccacgggtga ttagcgccgt acgcgcattc gcgccaaaac
    10081 cgataccgtc ggacatcccc gcgccaatgg caatgacgtt tttcaccgcg ccgccaagct
    10141 gcacgccaat gaaatccggg ttactgtaaa cgcggaaact tttaccgcag tgcagcagct
    10201 gctgaagatc atcagcaaaa gtttggtcgg tcgaggccag cgaaatggct gtcggtaaac
    10261 ctgccgccag ttctttcgca aacgttgggc cagagataac cgccagcgga atttgatcgc
    10321 ctaacgcctc gcgggccacg tcctgtaaca gacgcccggt ttccgcttcc agccctttgg
    10381 tcgcccacac cagacgcgca tcaggacgca tcagcggttt aatctggcgc agcacttcac
    10441 caaagacatg gctgggtacg acgacgagaa tattacggct ggctgccagc gcagtggcga
    10501 gatcgctttc aagatggagt gtatcgggaa aaggcacatc ggggagaaac gcggcgttac
    10561 agcggtcgcg ttcaagcgtt gcgatatgtt cagggtcatg gccccagagg acaacctcgt
    10621 ggccatttct tgccagggta atggcaagag cggtgccgta cgagccggca ccgatcacag
    10681 tcattgaagc attacgttgg ttcatcaggc atcctgatgt tcttcagtac cttcgccagc
    10741 ctgctgctgc aaatagttca tgaacagcgc atcgaagtta accggcgcaa ggttcagttg
    10801 cgggaatgta ccgcgggata ccatgctggt gatgcactca cgagcatacg ggaacagaat
    10861 gttcgggcag tatgcgccca ggcaatgtgc catctgggtg ccttcgatac ccgcgatgga
    10921 gaaaataccg ccctgctgaa cttcacacag gaacgcggtt tcttcgccca gggaggccgt
    10981 tacggtaaca cgcagtacca cttcgtaaac gtcatctgcc agttgagtag atgccgtatc
    11041 cagatcaagt ttaacttctg gctgccaatc tttctggaaa acgtgcggcg cgttcggcgc
    11101 ttcgaaagaa atatccttgg tataaatacg ttggatctgg aaagtcattt cggtgttgtt
    11161 ttgttctgac atgtgtagaa aacccttaag tgttgtcctt aaatactgcg caatgtaatg
    11221 ccaacgttca cacgttattt cagcagggga tccagtccac cacgtgcatc caatgcatac
    11281 aagtcatcac agccgccaat gtgctgtgcg tcaataaaaa tctggggaac cgtggtgcga
    11341 ccgctgcgtt tgatcatctc ttcacgctta gcggcgttgc catcgatcgg caactcctga
    11401 aaactcacgc ccttgctgct cagcagtgct tttgcacgat ggcaatacgg gcaggtttct
    11461 ttggtataga tttcaacatt ggccataact tagctcctgt tttttaccct gaaatttcat
    11521 gttgcagggc ggcagcaaaa tggagtgtcc ccagaagctt ataacaataa gtcgctgggg
    11581 gcctaattgc agctaacggc ctgcatcatg aaagacgaca ggtgaattat ttgccgcgca
    11641 ccaaaggcaa gttttcgcca gcccagccag cgacgccttc tttcagcacg aatacttgcg
    11701 caaaaccagc tttcgtcagt gcgtttgcag gctcctggca ctgcatgcca gaaccgtcta
    11761 ccacgataac cggtttgtct ttgtgcttct caagctcacc aacattgttg gctttgattt
    11821 cgctcggcaa cagattaata gaacctgcga tatggccttt acggaagtca tcacgctgac
    11881 gtaaatccac aaccacagcg tcttctttgt tgatcagacg cgtagcttca ccacgagtaa
    11941 tcaccttcac tttcgaggtc aggctcttaa acgtagtcac aagaaccgcc accagtaacg
    12001 cgatccaggc gatactcagt atgggatgac ggccaacaaa ttgcataatt tcttgcatgg
    12061 ggggtaacaa ctcccgacgt agtgattaaa aaaaccagga aaggagtata cctgcgcggt
    12121 gcggcaaata cagccagcgc gttaactgga atgcaatttt gcggggcgcg acgaaaaaaa
    12181 agcgtaaatc tggcatcggc ttgccgcgag cggtcgtata ttttgatctt cagaggctat
    12241 tttatcgatt cagctgtagt aaaattacgc aaattttgac tcttgagtat gaggttgtcg
    12301 caatgtcggt ttctaaaaaa cctatggtac tggtgattct ggatggctat ggctatcgcg
    12361 aagaacagca ggataacgcc atttttagtg ctaaaacccc ggtaatggat gcactgtggg
    12421 ccaatcgtcc gcataccctg atcgacgctt ccggtctgga agtcggtctg cctgaccgtc
    12481 agatgggtaa ctccgaagta ggccacgtta acctgggtgc cggccgcatc gtgtatcagg
    12541 acctgactcg tctggacgtt gaaatcaaaa atcgcgcttt ctttgctaat ccagtgctga
    12601 ctggcgcggt agataaagcg aaaaacgcag gcaaagcggt acacattatg ggtctgctct
    12661 ccgcaggcgg cgtacacagc cacgaagatc acatcatggc aatggtagaa ctggcagctg
    12721 aacgcggcgc agaaaaaatc tacctgcacg cattccttga cggtcgcgac actccgccgc
    12781 gcagtgctga atcctcgctg aaaaaattcg aagagaaatt tgccgcgctg ggtaaaggcc
    12841 gcgtagcgtc tatcattggt cgttactacg cgatggaccg cgataaccgt tgggatcgcg
    12901 ttgaaaaagc ttatgacctg ctgactctgg cgcagggtga gttccaggcc gataccgccg
    12961 ttgctggttt gcaggctgct tatgctcgtg acgaaaacga cgagttcgtg aaagcgaccg
    13021 ttattcgtgc agaaggtcag ccagatgcgg caatggaaga cggcgatgcg ctgattttca
    13081 tgaacttccg tgctgaccgt gcgcgtgaaa tcactcgtgc tttcgtgaac gctgatttcg
    13141 atggcttcgc gcgtaagaaa gtggttaacg tcgatttcgt gatgctgacc gaatacgccg
    13201 ctgacatcaa aactgcggtt gcttacccac ccgcttccct ggttaacacc ttcggcgagt
    13261 ggatggcgaa aaacgacaaa actcagttgc gtatttccga aaccgaaaaa tatgcccacg
    13321 ttactttctt ctttaacggt ggcgtagaag agtcgttcaa aggcgaagat cgcattctga
    13381 tcaactcacc gaaagtggct acctacgatc tgcaaccgga aatgagctcc gcagagctga
    13441 ccgaaaaact ggttgcggca atcaagagcg gcaaatacga caccattatc tgtaactatc
    13501 cgaacggcga catggtaggt cacaccgggg tgatggaagc agcagttaaa gccgttgaag
    13561 cgctggatca ctgcgtggat gaagttgcga aagcggttga gtctgtaggt ggacaactgc
    13621 tgatcaccgc tgaccacggt aacgctgaac agatgcgcga tccggcaacg ggtcaggcgc
    13681 acacagcgca caccaacctg ccagttccgc tgatttacgt tggcgataag aacgtgaaag
    13741 cggttgaagg cggcaaactt tctgacatcg cgccgaccat gttgtcgctg atgggtatgg
    13801 aaatcccgca agagatgact ggtaagccgc tgttcatcgt ggaataatcc ctccccatga
    13861 ggggaaaggc gattaatacc atgacacggg ccgtgaaacc gcgcaggttt gcaatcaggc
    13921 ccatcatcta cgccagcgtt cttagcgctg gcgtattgtt gtgcgccttt tccgcccacg
    13981 cggatgagcg tgaccaactc aaatctattc aggccgatat cgccgcgaaa gagcgcgcgg
    14041 tacgccaaaa gcaacaacaa cgcgcaagcc tgctcgcaca attgaaaaag caggaagaag
    14101 cgatctctga agccacccgt aagctgcgcg aaacgcaaaa cacgctcaat caactcaata
    14161 aacagattga tgagatgaac gcgtcgattg ccaaactgga gcagcaaaaa gccgcccagg
    14221 agcgcagcct cgccgcacaa ctggatgccg cattccgtca gggcgagcat accggtattc
    14281 agctgattct cagcggtgaa gaaagccagc gtggacagcg tttacaggct tatttcggct
    14341 atctcaacca ggcgcgacaa gaaaccattg cccagttgaa gcaaacgcgt gaagaagtcg
    14401 ccatgcagcg tgctgaactg gaagagaaac agagcgagca acaaacgctg ttatatgagc
    14461 agcgcgccca acaggcgaaa ctgactcagg cgctgaacga gcgtaaaaag acgctggcag
    14521 ggctggagtc ttccatccag caaggtcagc aacagttgag cgagctgcgc gccaacgaat
    14581 cccgtctgcg taacagcatt gcccgtgcgg aagccgcggc gaaagcgcgt gcagaacgag
    14641 aagcacgtga ggcccaggcg gttcgcgacc gccagaaaga agcgacgcgt aaaggcacca
    14701 cctacaaacc gactgaaagc gaaaaatcgc tgatgtcccg taccggtggt ctgggcgcgc
    14761 cgcgtggtca ggcattctgg ccggttcgcg ggccgacgct gcatcgctat ggcgagcagc
    14821 tacagggtga attacgctgg aaagggatgg tgattggtgc ctctgaaggt actgaagtta
    14881 aagcgattgc cgacggtcgg gtgattctgg ctgactggct gcaaggctac ggtctggtgg
    14941 tggtggttga gcatggtaaa ggcgacatga gtctttatgg ctataatcag agcgcactgg
    15001 tgagcgttgg ttcgcaggtt cgcgcgggcc agccaattgc actggtgggc agcagtggcg
    15061 gtcagggtcg gccttcactc tatttcgaaa ttcgccgcca gggtcaggcg gtcaatccac
    15121 agccgtggtt gggaagataa gttttgtttc catttcgtcg taacgttctt gcatttgccg
    15181 ctctgttggc gctctcctcc cccgtaattg ctggcaaact tgccatcgtc attgatgatt
    15241 ttgggtatcg cccgcacaac gaaaaccagg tgctggcgat gccttccgct atctccgtcg
    15301 ctgtattacc cgattcaccg aacgccagag agatggcgac caaagcgcat aacagcggcc
    15361 acgaagtgtt gattcatcta ccaatggcac cattgagtaa acagccgctg gagaaaaata
    15421 cgctacgccc ggagatgagc agcgacgaaa ttgagcgcat tattcgtagt gcggtcaata
    15481 acgtgcccta tgccgtgggg atcaacaacc acatgggcag caagatgacc tctaacctgt
    15541 ttggtatgca gaaagtgatg caggcgctgg agcgttacaa tctttacttc ctcgacagcg
    15601 taaccattgg taatacccag gcgatgcgcg ccgcgcaagg cactggcgtg aaggttatta
    15661 aacggaaggt gttcctcgac gattcacaga acgaagcgga catccgtgtg cagttcaatc
    15721 gcgcaattga tctggcgcgt cgcaacggtt caaccattgc cattgggcat cctcaccctt
    15781 caacggtacg cgtgctacaa cagatggttt ataacctgcc gccagacatt acgctggtga
    15841 aagccagcag cttgctgaat gaaccgcagg ttgatacgtc tacaccgccg aaaaacactg
    15901 tgcccgacac accgcgtaat ccgttccgtg gcgtgaagct gtgcaaaccg aagaaaccga
    15961 tagaacctgt ttatgctaat cgcttctttg aagtattaag cgaaagcatc agccagagca
    16021 cgctgatcgt ttacttccag catcagtggc aaggctgggg caaacagccc gaagcggcaa
    16081 aacttaacgc tagcgcaaat taaaggcgct acgcgtaatg cgatggtgcg accgcgtttt
    16141 gtcgcgccat tgccataagc ggaaagacca cagtaacgcc tggtaaccca ccttcacgct
    16201 gcgcacattg gtaatcaggc gcttatacat accggaagta aatatctcgg caatcatccg
    16261 ttggcgggta agaatatccg gctctttgcg caccgcatgg caaacacgca atgcttcgta
    16321 agttatttgc tgatgaaatt ccggataaat cgtaattttg tcggcataat ttcgatttaa
    16381 tttctccagc aggcgggtaa tcttaatata gtgacgttga taattaaggt ttttattccc
    16441 ttgtctatgc aaccgactca ctgacgtatt atgcagataa tatttatata atgattgctc
    16501 ggtatatcgc gcacgcagcg cgttaaacat gaattctgtt gtccagacaa tatcctgatg
    16561 atgtaatccg gcaataaatt taatgttatt tttaacaata acatcgcggc gataaacccc
    16621 catccagaca acgtgagtcc aacggcgcga agaaagcccc atccgcagcc agtccgggcc
    16681 ggttaataca ccggttgagc gaaggcgatc gctggggatg gattgccagg tttctcccgt
    16741 ttcacgaaaa caccagtcag cgttgcactg cgccacgtcg aggtcgtcct ctaacgccat
    16801 ggtcatcagc gtttcgtaca tggtgggata gacttcatcg tcagcatcga caaaagcgac
    16861 atatttgccc gttgccactt caatcccatg attacgcgcc accgatgcgc cagcattcgc
    16921 ctgatgcaac aaacgaacgt gcggatagtt ttctgcgtaa tgctttgcta tttcaacaga
    16981 attatccgtt gaaccatcgt taataataat gatttccaga gcagtccagg tttgcgtaat
    17041 taaagattcc atacaagtgc ggaaatcatc gcccgcatta tataacggaa taataacact
    17101 aagtttattg gtgctgttca tcataaatta ccgacgacct ctgaagttat cctgacgttt
    17161 tacaggcttt gtattaagaa actattaagc ctgtgagata ttctttttac aggtgagaga
    17221 gtcacttcag ggtagagaga taatgagagc agctatataa gagatcagca caattacgaa
    17281 ctctttatta cactgacagg attttacata tttaacagat tatcctcaga taaaagggct
    17341 ggaataccag cccttgttcg tgttaatccc agctcagaat aactttcccg gactggcccg
    17401 aacgcatagc gtcaaagccc ttctggaaat catcgataga gaaacgatgg gtaatgatcg
    17461 gcgagagatc gaggccagac tgaatcagcg ccgccatctt gtaccaggtt tcaaacatct
    17521 cacgaccgta aatgccttta atgaacaagc ccttaaagat cacttttgtc cagtctatcg
    17581 acatgtcaga aggtggaata cccagcatcg caatacgacc gccgtggttc atggtgtcga
    17641 gcatggtacg aaacgctggc ggcgcaccgg acatttccag accgacatca aagccttcgg
    17701 tcatgcccag ttctgccatc acatcattaa ggttttcttt agcgacgttg accgcacggg
    17761 tgatacccat tttgcgcgcc agttcgagac gatattcatt aacatcagtg atcaccacat
    17821 tgcgtgcacc aacgtgtttc gccaccgccg cagccataat acctatcgga cctgcaccag
    17881 agaccagcac atcctcgcca accagatcga acgacagcgc ggtatgcacg gcgttaccga
    17941 agggatcaaa aatggaagcc aggtcgtcgg agatattgtc gggaattttg aaagcgttga
    18001 acgccggaat caccagatat tcggcgaagc agcccggacg gttaacgccg acgccgatcg
    18061 tgttgcggca cagatgggta cgaccgccac gacagttgcg acagtgaccg caagtgatat
    18121 gaccttcgcc agaaacgcga tcgccaatct tgaagccttt cacttcctga ccaataccta
    18181 ccacttcacc gacatattca tggcccacga ccatcggcac cgggatggtt ttttgcgacc
    18241 actcatccca gttatagatg tgaacgtcag tcccgcagat ggctgtttta cggattttaa
    18301 tcagcagatc gttatgcccg agttccggta caggaacgtc ggtcatccag atgccctctt
    18361 ccgctttcag tttggataac gctttcatct cacatcctca ggcgataacg cccagttgtt
    18421 taccaatacg cgtgaatgct tctactgcac gcgtaatttg ctcaggggta tgcgccgcag
    18481 acatctgggt acgaatacgc gcctgacctt tcggaacgac cggatagaag aaaccggtga
    18541 cgtaaatgcc ctctttttgc agctcacggg caaatttctg cgccactacc gcatcaccaa
    18601 gcatgaccgg aataatggcg tgatcggctc ccgccagggt aaagcccgcc gccgacattt
    18661 gctcacggaa ctgacgcgcg ttcgcccaca gacggtcacg cagctcgcta cccgcttcga
    18721 ccatctccag tactttgatg gatgcggcaa caatggccgg tgccagcgag ttggagaaca
    18781 gatacggacg agaacgctgg cgcagccact caaccacttc tttgcgcgcc gcagtataac
    18841 caccagaagc cccgcccagc gctttaccaa gcgtaccggt gataatatcg acccggccca
    18901 tcacatcgca gtattcatgg gaaccacgac cgttttcacc gacaaaaccg accgcgtggg
    18961 agtcgtctac catcaccagg gcatcgtatt tatctgccag atcgcaaacg cccttcagat
    19021 tggcaatcac gccgtccatc gagaacacac catcggtggc aatcagcaca tgacgcgcac
    19081 cggcttcacg cgcttctttc agacgagctt caagctcctg catatcgttg ttggcatagc
    19141 gatagcgttt agctttacac agacgcacac catcaataat agacgcgtgg ttcagtgcgt
    19201 cggagataat ggcgtcttcc gcgcccagca gcgtttcaaa caggccaccg ttagcatcaa
    19261 agcaggaaga gtagagaatc gcatcttcca tccccaggaa tgccgccagt ttttgttcaa
    19321 gctctttatg gctgtcctga gtgccgcaaa taaaacgcac cgaagccatg ccgaaaccgt
    19381 gagaatccat tcccgccttt gccgccgcaa tcagatccgg atgattcgcc agcccgagat
    19441 agttattggc acaaaagtta atgacgtggc ttccatcagc cacagtgata tctgcttgct
    19501 gcgcagacgt aataatgcgc tcttctttaa acaacccttc cgcccgtgcg gtttccagat
    19561 cgttggttaa ctgctgataa aattctccac gcattgcgat tctccagaca gggcaaattc
    19621 cagcacatat tacccaaact tataggtcgc gacgagatga cgcgttaaca cttctgcaaa
    19681 attcaggata aataacgtgt atcttgatgt ctctcaacac ctacagatag tagaaaaact
    19741 gtacagtttt ggctgagcat aatgatgagt gattttactg cattgatata aaaagaaaaa
    19801 atgatgccaa aacatcattt ttcctatctc agttggcatc aaaacgctac ccactaatgg
    19861 cgggaaacct attttcttga aaggaaaatt ctcagcgcct gcatttttgc ccctaaagtg
    19921 ttactcctga agcagcgaaa caaatcgaac cattttcctc tccccagata gtttaggttg
    19981 aagagaccgg aacgtttgta atagcacatg acaaaaatgc cctggtcatc gtccacaatg
    20041 ttattgttta gtgttatttt ttgactttcc agaactaatt tatagaattc tttccattta
    20101 tactgtgaac caacaattgc gcccccaata atatatacat gattgccaat catgaaatca
    20161 aaagcctgtt gttgagatgt aacagtaagc ccttttttta tagtgaaaag atgcatctta
    20221 ttttcatcaa atgggaaatc ccaaattttc aatcctcgag tcacatttgg cttacggcaa
    20281 tagccaaaat ctatccacgc aacaagaggc gttttcacta agcccatgtt gatagcttta
    20341 tttacaaaat aggccttaag gttacatacc aaaacatact ctggtgacca gtactctggg
    20401 ttttttaatt gtcgaggctc aagtctattt gtaaacgact catctttttg aattttttcg
    20461 atacgacttc tgatatgtct aaatttcttt tttatatcga tagcgataac cgttgttggt
    20521 tttccgttgc gaatcgcttc aacccttggt ttcaaatcgg gagaagtgaa aataatcatc
    20581 tcattttcaa gtacggccag acgctcaaag taactaaaat aaacatcaac tgaacgagct
    20641 agtttttcac ggaacccttt attagcagtc caatcccctc taccaatgtc aaaatatgca
    20701 gtgataattg tggtggatga tttcattact aataagacca ttaatataaa aaaataatta
    20761 t
//
LOCUS       DAZFKF010000087        20653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFKF010000087 DAZFKF010000000
VERSION     DAZFKF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.087"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            127..651
                     /gene="lpfA1"
                     /locus_tag="ACHKA1_003776"
     CDS             127..651
                     /gene="lpfA1"
                     /locus_tag="ACHKA1_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HFO5681368.1"
                     /translation="MKKVVFALTALALTSGSAFAAESGDGTVKFTGEIVDSPCVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFSGVGDADQSDL
                     ISVSTEAGAAKGVGIGIYDNSNTLVALNGGKASVDLSKGQTVLYFTANYVSTLATVTT
                     GYGNAQVDFNLSYE"
     gene            716..1402
                     /gene="lpfB"
                     /locus_tag="ACHKA1_003777"
     CDS             716..1402
                     /gene="lpfB"
                     /locus_tag="ACHKA1_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO5681369.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSATRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFRLDPGKNNILRIVNTTPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLQGDVKTAPNELRFTRSGNQI
                     RVDNPTVFNITFNQFFANEKEIEKAGMVPAKGSLNITLPAGAGSVSKIKYNAINDFGS
                     SAEMMTKNVD"
     gene            1431..4004
                     /gene="lpfC"
                     /locus_tag="ACHKA1_003778"
     CDS             1431..4004
                     /gene="lpfC"
                     /locus_tag="ACHKA1_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO5681370.1"
                     /translation="MSRKTVSRTFSSFSISVVAVAVASTFSAHAGKFNPKFLEDVQGV
                     GQHVDLTMFEKGQEQQLPGIYRVSVYVNEQRMETRTLEFKEATEAQRKAMGESLVPCL
                     SRTQLAEMGVRVESFPALNLVPAEACVPFDEIIPQASSHFDFSEQKLVLSFPQAAMHQ
                     VARGTVPESLWDEGIPALLLDYSFSGSNSEYDSSGSSSSYVDDNGTVHHDDGNDTLKS
                     DSYYLNLRSGLNLGAWRLRNYSTWSHSGGKAQWDNIGTSLSRAIIPFKAQLTMGDTAT
                     AGDIFDSVQMRGAMLASDEEMLPDSQRGFAPIVRGIAKSNAEVSIEQNGYVIYRTYVQ
                     PGAFEINDLYPTANSGDLTVIIKEADGSEQRFIQPFSSVPIFQREGHLKYSFAAGEYQ
                     AGNYDSASPRFGQLDLIYGLPWGMTAYGGVLISNNYNAFALGIGKNFGYIGAISIDVT
                     QAKSELNNDRDSQGQSYRFLYSKSFESGTDFRLAGYRYSTSGFYTFQEATDVRSDADS
                     DYNRYHKRSEIQGNLTQQLGAYGSVYLNLTQQDYWNDAGKQNTVSAGYNGRIGKVSYS
                     IAYSWNKSPEWDESDRLWSFNISVPLGRAWSNYRVTTDQDGRTNQQVGVSGTLLEDRN
                     LSYSVQEGYASNGVGNSGNANVGYQGGSGNVNVGYSYGKDYRQLNYSVRGGVIVHSEG
                     VTLSQPLGETMTLISVPGARNARVVNNGGVQVDWMGNAIVPYAMPYRENEISLRSDSL
                     GDDVDVENAFQKVVPTRGAIVRARFDTRVGYRVLMTLLRSAGSPVPFGATATLITDKQ
                     NEVSSIVGEEGQLYISGMPEEGRVLIKWGNDASQQCVAPYKLSLELKQGGIIPVSANC
                     Q"
     gene            4019..5074
                     /locus_tag="ACHKA1_003779"
     CDS             4019..5074
                     /locus_tag="ACHKA1_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5681371.1"
                     /translation="MKAAIALSLLGCVFGFSGKAFAGDAWGPCTPADGTTYHYNVDVD
                     VGIPDAAKNVAGTVLPDVLNWSNGQNVSLICECPDSYKNEKDTLVQGVSMLPPSGRSV
                     DSMKYYTLTEELEVATKIRISTSVYGFVPFKNQQALQTTGCNKVITTPYMGGAGLLSF
                     AITKPFIGDSVIPLTLIAELYASKTNKDYGTIPISSVSIQGRVTITQDCEIKPGTVLD
                     VPFGEFPSSAFKNRQGQMPEGATEQEINLGFDCNNISDGIKVSLRLEGATNADDPRAV
                     DMGNPDIGVLVKDSSGKILVPNDASSTTLLNLSSLDSKTHRNAAIRLLALPISTTGKA
                     PKGGTFEGVTTIYLEME"
     gene            5079..5609
                     /gene="lpfE"
                     /locus_tag="ACHKA1_003780"
     CDS             5079..5609
                     /gene="lpfE"
                     /locus_tag="ACHKA1_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO5681372.1"
                     /translation="MKFNLLLHSGIASLSLFACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            5864..7555
                     /gene="eptB"
                     /locus_tag="ACHKA1_003781"
     CDS             5864..7555
                     /gene="eptB"
                     /locus_tag="ACHKA1_003781"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO5681373.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAVAN"
     gene            7647..7723
                     /locus_tag="ACHKA1_003782"
     tRNA            7647..7723
                     /locus_tag="ACHKA1_003782"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7681..7683,aa:Pro,seq:cgg)
     gene            8468..10075
                     /gene="dppA"
                     /locus_tag="ACHKA1_003783"
     CDS             8468..10075
                     /gene="dppA"
                     /locus_tag="ACHKA1_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO5681374.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVAA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10383..11402
                     /gene="dppB"
                     /locus_tag="ACHKA1_003784"
     CDS             10383..11402
                     /gene="dppB"
                     /locus_tag="ACHKA1_003784"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO5681375.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRLRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11412..12314
                     /gene="dppC"
                     /locus_tag="ACHKA1_003785"
     CDS             11412..12314
                     /gene="dppC"
                     /locus_tag="ACHKA1_003785"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO5681376.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12325..13308
                     /gene="dppD"
                     /locus_tag="ACHKA1_003786"
     CDS             12325..13308
                     /gene="dppD"
                     /locus_tag="ACHKA1_003786"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO5681377.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13305..14309
                     /gene="dppF"
                     /locus_tag="ACHKA1_003787"
     CDS             13305..14309
                     /gene="dppF"
                     /locus_tag="ACHKA1_003787"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO5681378.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14339..15610)
                     /gene="yhjV"
                     /locus_tag="ACHKA1_003788"
     CDS             complement(14339..15610)
                     /gene="yhjV"
                     /locus_tag="ACHKA1_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO5681379.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            16086..16193
                     /locus_tag="ACHKA1_003789"
     CDS             16086..16193
                     /locus_tag="ACHKA1_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5681380.1"
                     /translation="MTLAELGMAFWHDLVAPVIAGILASMIVNWLNKRK"
     gene            16569..16676
                     /locus_tag="ACHKA1_003790"
     CDS             16569..16676
                     /locus_tag="ACHKA1_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5681381.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(16763..18442)
                     /gene="bcsG"
                     /locus_tag="ACHKA1_003791"
     CDS             complement(16763..18442)
                     /gene="bcsG"
                     /locus_tag="ACHKA1_003791"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO5681382.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(18439..18630)
                     /gene="bcsF"
                     /locus_tag="ACHKA1_003792"
     CDS             complement(18439..18630)
                     /gene="bcsF"
                     /locus_tag="ACHKA1_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO5681383.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(18627..20198)
                     /gene="bcsE"
                     /locus_tag="ACHKA1_003793"
     CDS             complement(18627..20198)
                     /gene="bcsE"
                     /locus_tag="ACHKA1_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO5681384.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            20471..>20653
                     /gene="bcsR"
                     /locus_tag="ACHKA1_003794"
     CDS             20471..>20653
                     /gene="bcsR"
                     /locus_tag="ACHKA1_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO5681385.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQ"
BASE COUNT         5185 a         5033 c         5445 g         4990 t
ORIGIN      
        1 tattgatatt ttatcctctt aattttcttt tccttgttta tttgtataat cccgccttca
       61 aagttgatgc aatgttaaag catcactatt tgctaattaa tgtgatttaa taaaatggag
      121 tttttcatga aaaaggtcgt ttttgcctta actgcactgg cgttaacctc cggctctgca
      181 tttgcagcag aatctggtga cggcacggtt aaatttacag gcgagatcgt ggattcacct
      241 tgcgtactgt ccgttgactc tcagaaccag gaagttgtgt tgggtcaggt acaaaaatct
      301 gtatttgctg cggttggtga caaatccccg gcaaaacctt tcgaaatcaa actggaagat
      361 tgcgatacca ccacgatgaa aaaagccaat gtgagcttta gcggtgttgg tgatgcagat
      421 caatctgatc tgatttctgt tagtactgaa gccggtgcgg cgaaaggtgt tggtatcggt
      481 atttatgata acagcaacac cctagtcgct cttaacggtg gtaaagcaag tgttgatctg
      541 agtaaaggcc agacggttct ctactttacc gccaactacg tttcaactct ggcaacggta
      601 acgaccggtt acggcaatgc gcaggtcgat ttcaacctgt cttacgagta atcgtctgtt
      661 ttacccatgg cggcgtaatt gccgccatta gtcgtcaaaa ttcatggatc gaatgatgaa
      721 aagtaaattt gttgcgttgg ctctgtctct gttcctctcc cagtcagttc tggcgggggg
      781 cgttgggctt agtgctaccc gtgtgattta tgatggcagt aagaaagaag cctcgctgac
      841 cgtacagaat aaaagtaaga cggaagagtt tctgatccag tcctgggtgg atgatgccgc
      901 gggcagcaaa aaaacaccgt ttataattac cccgccgttg ttcaggctcg atcctggaaa
      961 aaacaatata ttgcgtattg taaatactac ccctggcctg ccgcaggatc gcgaatcggt
     1021 ctactgggtt aatgtgaaag ccattccgtc taaaagtgac gacagcgaaa acaaaaatgt
     1081 attacagatt gctgttcgca cccgcatcaa actgttttat cgcccggcag ggttacaggg
     1141 tgatgtaaaa accgcgccaa acgaactgcg atttacgcgt agcggtaatc agatccgtgt
     1201 cgataacccc accgttttta atattacctt taatcaattt tttgcgaatg aaaaagaaat
     1261 tgaaaaagcc ggcatggtac ccgcaaaagg gtcattaaat attacattac ccgccggggc
     1321 aggtagcgtt tcgaagatta aatataacgc catcaatgac tttggatcgt cagcggaaat
     1381 gatgactaaa aatgttgatt gattaatacc ttttcaggag aaattgctca atgtcacgga
     1441 agaccgtttc acgtactttt tcttcctttt ctatctctgt ggtggcggtt gctgttgcca
     1501 gcacgttttc tgcacatgcc ggaaagttta accccaaatt cctggaggac gttcagggtg
     1561 tgggccagca tgtcgattta acgatgttcg aaaaaggcca ggaacagcag ttacccggca
     1621 tctatcgtgt ctctgtttac gtcaatgaac agagaatgga aacccgcaca ctggaattta
     1681 aagaggccac agaagcacag cgcaaagcga tgggggaatc ccttgtgcct tgccttagcc
     1741 ggacgcaact ggcagagatg ggcgtgcgcg ttgaaagctt tccggccctc aatctggtgc
     1801 ctgccgaagc ctgcgtgccg tttgacgaaa tcattcccca ggcctcaagc catttcgact
     1861 tcagtgaaca aaagctggta ttaagtttcc ctcaggcggc gatgcaccag gtggcgcgcg
     1921 gcacggtgcc ggaatcttta tgggacgaag gtatcccggc attattgctg gattacagtt
     1981 ttagcgggag caacagtgaa tatgactcct cgggttcttc cagcagttat gtcgatgata
     2041 atggcaccgt tcatcatgat gatggcaacg atacgctaaa gagcgacagc tactacctca
     2101 acttacgcag cggcctgaac cttggcgcgt ggcggttacg taattacagt acctggagcc
     2161 attccggtgg caaagcgcag tgggataata tcggtacgtc tttgagtagg gccatcatcc
     2221 ctttcaaagc gcaattaacg atgggggata cggcgaccgc gggggatatt tttgacagcg
     2281 ttcagatgcg tggcgcgatg ttagcctctg atgaagagat gctgcccgac agccagcgag
     2341 gctttgcacc catcgttcga gggatcgcca aaagcaacgc cgaagtcagt attgaacaaa
     2401 acggctacgt tatttatcgc acctatgtgc agcctggtgc ctttgaaata aacgatcttt
     2461 accccactgc aaacagcggt gatctcacgg tcattattaa agaagcggat ggcagtgaac
     2521 aacgctttat tcagccattc tcatcggtgc caattttcca gcgtgaaggc catctcaaat
     2581 atagctttgc cgcaggtgaa tatcaggccg ggaattatga cagcgcctcg ccgcgtttcg
     2641 ggcagcttga tctgatctac ggtttaccgt gggggatgac ggcctacggc ggcgtattaa
     2701 tctctaataa ttacaatgca tttgcattag ggatagggaa aaactttggt tatatcgggg
     2761 cgatttccat tgatgtgacg caggctaaaa gcgaactgaa taacgatcgc gatagccagg
     2821 gacaatctta tcgtttctta tattccaaga gcttcgaaag cggcaccgat ttccgccttg
     2881 cgggctatcg gtactctacc agcggtttct ataccttcca ggaagccacc gatgtgcgca
     2941 gtgacgctga cagcgactat aaccgttatc acaagcgcag cgaaatacag ggtaacctga
     3001 cgcagcaatt aggggcctat ggctctgttt atttaaattt aacgcagcag gattactgga
     3061 acgacgcagg taaacagaac acggtatcgg cgggttacaa cggacgtatt ggcaaggtca
     3121 gttacagtat tgcatatagc tggaataaaa gccctgaatg ggatgaaagc gatcgcttgt
     3181 ggtctttcaa tatttccgtt ccactaggcc gggcctggag taactatcgc gtcacgaccg
     3241 accaggatgg tcgtaccaat caacaggttg gggtcagcgg aacgctgctt gaggatcgca
     3301 acctgagcta cagtgttcag gaaggctacg ccagcaacgg tgtgggtaac agcggtaacg
     3361 ctaacgttgg ctatcagggt gggtccggta atgtcaacgt aggctatagc tacgggaaag
     3421 attaccggca gctcaactac agcgttcgcg gcggcgtgat agttcatagc gaaggcgtga
     3481 cgctttccca accgctaggc gaaaccatga cgctcatctc cgtacccggt gcgcgcaatg
     3541 cccgcgtggt gaataacggc ggcgttcagg ttgactggat gggtaacgcg atcgtgcctt
     3601 acgccatgcc gtatcgtgaa aacgaaatct cactgcgtag cgattcgttg ggtgacgatg
     3661 ttgacgttga aaatgcgttc cagaaagtgg tgccaacgcg tggagcgatt gtcagagcgc
     3721 gttttgatac ccgcgttggt taccgcgtat taatgacgct gcttcgttcc gcgggcagcc
     3781 cggtgccctt tggagcaacg gcaacgctaa tcaccgataa acaaaacgag gtgagcagta
     3841 tcgttggtga agaaggacag ctctatatta gcggaatgcc agaggaagga cgggtattga
     3901 ttaaatgggg taatgacgcg tcgcagcaat gcgtggcgcc ttataaatta tccctggaat
     3961 taaaacaggg cggaattatt cctgtttcgg ccaattgcca gtaagcaagg agagcgaaat
     4021 gaaagcggca atagcattat ccttattagg atgcgtattc ggattttctg gcaaggcatt
     4081 tgccggagat gcgtgggggc catgtacccc cgcggatggc acgacttatc actataatgt
     4141 cgatgtggat gttggtatac ctgatgcggc aaaaaacgtc gcaggaacgg ttttaccgga
     4201 tgtccttaac tggtctaatg gacaaaacgt atcgctaatt tgtgaatgtc ctgattctta
     4261 taagaatgaa aaagatactc tagtacaagg ggtgagtatg cttccccctt ctggccgttc
     4321 ggttgacagt atgaaatatt atactttgac ggaggaactg gaggttgcga cgaaaattcg
     4381 catcagtacc agtgtgtatg gatttgttcc tttcaaaaac cagcaggcgc tgcaaacaac
     4441 cggatgcaac aaagtcatta ctacgcccta tatgggcggc gcaggtctac tttcctttgc
     4501 tattactaaa ccctttatcg gcgattccgt catccctctg acattaattg ccgaactgta
     4561 tgcctccaaa acaaataaag attacggaac aatacccata tcttccgtat ctattcaggg
     4621 ccgcgtcacc ataactcagg attgcgaaat aaagcccggc acggtgctgg atgtgccatt
     4681 tggtgaattc ccctcctcag cgtttaaaaa cagacaaggg caaatgcctg aaggtgcgac
     4741 agagcaagaa attaacctcg gctttgattg caacaatatc tcggatggta tcaaagtctc
     4801 gttgcgcctt gagggggcaa ccaatgctga cgatccccga gcggtggata tgggcaaccc
     4861 cgatattggc gtactggtga aagattccag cggcaaaatt ttggtaccga atgatgcaag
     4921 cagcaccacg ttattaaatt tgagcagcct ggattctaaa actcaccgca atgccgcgat
     4981 tcgactgctg gctttaccga ttagcacgac gggtaaagcg cctaaaggcg ggacgtttga
     5041 aggcgtaacg accatatatc ttgaaatgga gtgatgccat gaaattcaat ctattgctgc
     5101 atagcggcat cgccagtttg agtctgtttg cctgcggggt gaatgcggcg acggatcttg
     5161 gcccggcagg ggatattcat ttctccatca ctatcaccac taaagcttgc gagatggaaa
     5221 aaagcgatct cgaagtcgat atgggaacaa tgacgctgca aaaacctgcg gcagtcggta
     5281 cggtgttgag caagaaagat ttcaccattg aactcaaaga gtgcgatggg atatccaaag
     5341 cgaccgttga gatggacagt cagtcggaca gcgatgatga ttccatgttt gcccttgagg
     5401 ctggtggcgc aacgggtgtt gcgttgaaga tagaggacga taaaggaacg cagcaagttc
     5461 ccaaaggctc cagcggaacg ccgattgaat gggcgattga tggcgaaacc acgtcgcttc
     5521 actaccaggc gagttatgtg gtcgtcaaca ctcaggccac tggtggcaca gcgaatgccc
     5581 ttgtcaattt ttccatcacc tatgagtaag ttactgggcc agcatcctgg cccaacctat
     5641 ctctgaatag tcgttaatcc gttaaaatgg tctattccgg acttgcactt ttgtaaattt
     5701 gtgcttcatg cacactcttt ccccacactt tttccctttg ctgtggtcta cttattcgcg
     5761 cgtgtagatt ttacttatct gactacctcc gcacttttcc cctgctgggc ctgaaaagcc
     5821 gctaagcagg gtgttatcac ctgtttgtcc agggtttgtt tgcatgagat acatcaaatc
     5881 gattacacag cagaagctga gctttttgct tgcaatctat attggccttt ttatgaatgg
     5941 cgcggttttt taccgccgct tcggcagcta tgcgcacgat tttaccgtct ggaaaggcat
     6001 ttctgctgtt gttgaactgg ccgccaccgt actggtgacc ttctttttac tacgtcttct
     6061 ttcgctgttt ggccgccgca gttggcgtat tctggcatcg ctggttgtgc tcttttccgc
     6121 aggtgccagc tattacatga ccttccttaa tgtggtcatt ggttatggca tcatcgcttc
     6181 cgtcatgacc accgatatcg acctgtcaaa agaagttgtt ggtctgaact ttattctctg
     6241 gttaatcgcc gttagcgcat tgcctcttat ccttatctgg aataaccgct gtcgctacac
     6301 cttgctccga caactgcgaa ccccagggca gcgtattcgc agcctggcgg tcgtcgtact
     6361 ggcgggaatt atggtttggg caccgattcg tttgctggat atccagcaga agaaagtgga
     6421 gagggcgacc ggcgttgatt tgccgagtta tggcggtgtc gtagcgaact cttatctgcc
     6481 atcaaactgg ctttctgcgt tggggctgta cgcctgggcg cgggtcgatg aatcttccga
     6541 taataattca ttgcttaacc cggcgaagaa attcacttat caggcaccgc aaaatgttaa
     6601 tgacacttat gtcgtgttta tcatcggtga aactacgcgt tgggaccata tgggcatttt
     6661 cggctatgag cgtaatacca cgccgaagct ggcccaggag aaaaatctgg cggcgttccg
     6721 tggttactcc tgtgataccg caaccaaact ctctctgcgt tgcatgtttg tacgtcaggg
     6781 gggcgcggaa gataatccgc agcgcacatt aaaagaacag aacattttcg cggtactgaa
     6841 gcagttagga ttcagttctg acctctacgc tatgcagagc gaaatgtggt tctacagcaa
     6901 tacgatggcg gacaatattg cttaccgtga acagattggt gcggagccac gtaaccgtgg
     6961 caagccggta gatgatatgt tgctggtaga cgaaatgcag caatcgctgg ggcgcaaccc
     7021 ggatggtaag catctaatca ttctgcatac caaaggttcg cactttaact acacccagcg
     7081 ttacccgcgc agctttgcgc agtggaagcc ggaatgtatt ggtgttgata gcggatgtac
     7141 caaagcgcag atgatcaact cctatgacaa ctcggtgacc tatgtggatc actttatctc
     7201 cagtgtgatt gatcaggttc gcgataagaa agcgattgtg ttctacgcag ctgaccacgg
     7261 cgagtcaatt aatgaacgcg agcacctgca cggcacgccg cgtgaactgg caccgccgga
     7321 gcagttccgc gtaccgatga tggtctggat gtcagataaa tatctggaaa atccggtcaa
     7381 tgcgcaggcg tttgcgcagc tgaaaaaagc agccgacatg aaagtgccac gccgtcacgt
     7441 agagctgtac gacaccatca tgggttgtct tggctatact tcaccggatg gtggaattaa
     7501 cgaaaacaac aactggtgtc acatcccgca gacaaaagag gcagtggcta actaagaggc
     7561 ctgctgactt tctcgccgat caaaaggcat tttgctatta agggattgac gagggcgtat
     7621 ctgcgcagta agatgcgccc cgcattcggt gattggcgca gcctggtagc gcacttcgtt
     7681 cgggacgaag gggtcggagg ttcgaatcct ctatcaccga ccaaattcga aaagcctgct
     7741 caacgagcag gcttttttgc atctgcagcc catgaggaag agaacctccg ggggcaggga
     7801 ggttcgactc gacgtaggcc tgataagacg cgtcagcgtc gcatcaggca acggcaccgc
     7861 acttattgtc ggatgcggcg tgaacgcctt attcgaccta cacaccacac atttccctgc
     7921 caaatttgcg ccccatatct cactatccat taacggattt gtgacagaaa ccattgtctt
     7981 tttttatatt tgcttaaatc tttttttgcg ttacttatgg ctggcgctgg cattttccgc
     8041 tgtgcgtttt agcgttcatg gtattaatcg ctcagatatc caccttttcc tcagattttt
     8101 ttcattaaat gcataaaact taatcaccaa atgttgctaa atattaagct gattgttctg
     8161 gtcatgttgg agtagcacaa atttctctgc tgcaaatagc tgtttgaagt ttttttaatc
     8221 tttgtttgtg gattctcacc gttggtgtaa atcccggttg gctgtattga cgttttcaca
     8281 ttctgttgac agattgtagg tcacgagggg cattttatgg aggatccgca ctgttacact
     8341 gatgttaatt agtacggcat ccccacctca taacgttgac ccgaccgggc aaaaaacaaa
     8401 aaaggtcagg cagcgacaac ccactgcaaa gggttaaaac aacaaacatc acaattggag
     8461 cagaataatg cgtatttcct tgaaaaagtc agggatgctg aagcttggtc tcagcctggt
     8521 ggctatgacc gtcgcagcaa gtgttcaggc taaaactctg gtttattgct cagaaggatc
     8581 tccggaaggg tttaacccgc agctgtttac ctccggtacc acctatgacg cctcttccgt
     8641 accgctttat aaccgcctgg ttgaatttaa aatcggcacc accgaagtga tcccgggcct
     8701 cgctgaaaag tgggaagtca gcgaagacgg taaaacctat accttccatc tgcgtaaagg
     8761 tgtgaagtgg cacgacaata aagaattcaa accgacgcgc gaaatgaatg ccgatgacgt
     8821 ggtgttctcg ttcgatcgtc agaaaaacgc gcaaaacccg taccataaag tttctggcgg
     8881 tagctacgaa tacttcgaag gcatgggctt gccggagctg atcagcgaag tgaaaaaggt
     8941 ggacgacaac acagttcagt tcgtgctgac tcgcccggaa gcgccgttcc tcgctgacct
     9001 ggcaatggac ttcgcctcta ttctgtcaaa agaatatgct gacgcgatga tgaaagccgg
     9061 tacaccggaa aaactggacc tcaacccaat cggaaccggt ccgttccagt tacagcagta
     9121 ccaaaaagat tcccgtatcc gctacaaagc gtttgatggc tactggggca ccaaaccgca
     9181 gatcgatacg ctggttttct ctattacccc tgatgcttcc gtgcgttacg cgaaattgca
     9241 gaagaatgaa tgccaggtga tgccgtaccc gaacccggca gatatcgctc gcatgaagca
     9301 ggataaatcc atcaacctga tggaaatgcc ggggctgaac gtcggttatc tctcgtataa
     9361 cgtgcagaaa aaaccactcg atgacgtgaa agttcgccag gctctgacct acgcggtgaa
     9421 caaagatgcc atcattaaag cggtttatca gggcgcgggc gtggcagcga aaaacctgat
     9481 cccgccaacc atgtggggct ataacgacga cgttcaggac tacacctacg atcctgaaaa
     9541 agcgaaagcc ttgctgaaag aagcgggtct ggaaaaaggt ttctccatcg acctgtgggc
     9601 gatgccggta caacgtccgt ataacccgaa cgctcgccgc atggcggaga tgattcaggc
     9661 agactgggcg aaagtgggcg tgcaggccaa aatcgtcacc tacgaatggg gtgagtatct
     9721 caagcgtgcg aaagatggcg agcatcagac ggtaatgatg ggctggactg gcgataacgg
     9781 ggatccggat aacttcttcg ccaccctgtt cagctgcgcc gcctctgaac aaggctccaa
     9841 ctactcaaaa tggtgctaca aaccgtttga agatctgatt caaccggcgc gtgctaccga
     9901 cgaccataac aaacgcgttg aactgtacaa gcaagcgcag gtggtgatgc acgatcaggc
     9961 tccggcactg atcatcgctc actccaccgt gtttgaaccg gtacgcaaag aagttaaagg
    10021 ctatgtggtt gatccattag gcaaacatca cttcgaaaac gtctctatcg aataattaaa
    10081 agccatacaa gactgatggc aaaggcaaaa ctgcctgatg cgctccgctt atcaggccta
    10141 cgaaaattct gcaatgtatt gagtttgcaa gattttgtag gccggataag gcgttaacgc
    10201 cgcatcccgc ataaacaaag cgctctttgt caacaatctg catacccggt ggtgctgtgc
    10261 ctctgcgtgc agcgccatcc ggcagcaata cttcattccc tgtcagacaa gggcgggaat
    10321 tgatttgtga gcaatacaga cacgcagttc caggctgcgg gtcactacag agaatccggg
    10381 ttatgttgca gtttattctc cgacgtttgg gactcgtcat ccccacgttt atcggtatta
    10441 cccttctcac atttgccttt gtccacatga tcccgggcga tccggtgatg atcatggcgg
    10501 gcgagcgtgg gatctcccca gagcgtcacg cgcagctgct ggctgaactc ggcttagata
    10561 aaccgatgtg gcagcagtat ctccattaca tttggggcgt tatgcatggc gatctaggca
    10621 tttcaatgaa aagccgcatc ccggtttggg aagagttcgt gccgcgcttc caggccacgc
    10681 tggaacttgg cgtctgcgcg atgatttttg ctacggcagt cggtattccg gttggtgtgc
    10741 tggctgcggt taaacgcggt tccattttcg atcacacagc ggttggcctg gcgctgacag
    10801 gttattcaat gcctatcttc tggtggggca tgatgctgat catgctggtt tcggtgcact
    10861 ggaacctgac acccgtctcc ggtcgcgtga gcgatatggt gttcctcgat gattccaatc
    10921 cgttaaccgg ttttatgctg atcgacaccg ccatctgggg tgaagacggt aactttatcg
    10981 atgccgtcgc ccatatgatc ttgcccgcca ttgtgctggg tactattccg ctggcggtca
    11041 ttgtacgtat gacacgctcc tcgatgctgg aagtgctggg tgaagattac atccgcaccg
    11101 cgcgcgccaa aggattgacc cgccttaggg tgattatcgt ccatgcgctg cgtaacgcga
    11161 tgctgccggt agtgaccgtt atcggcctcc aggtgggaac attgctggcg ggggcgattc
    11221 tgaccgaaac catcttctcg tggcccggtc tggggcgctg gttgattgac gcactgcaac
    11281 gccgcgacta tccggtagtg cagggcggcg tattgctggt ggcgacgatg attatcctcg
    11341 tcaatctgct ggtcgatctg ctgtacggcg tggtgaaccc gcgtattcgt cataagaagt
    11401 aaggggacat catgtcacag gttactgaaa ataaagtgat tagcgcaccg gtgccgatga
    11461 ccccgttaca ggagttctgg cactatttta aacgcaacaa aggcgcggtc gtcgggctgg
    11521 tttacgtcgt catcgtgctg tttatcgcga tctttgccaa ctggattgct ccctataacc
    11581 cggcggaaca attccgcgat gcgctgcttg ccccgcccgc ctggcaggaa ggcggcagca
    11641 tggcgcactt gctgggcacc gatgacgtag gccgtgatgt gctgtcgcgg ctgatgtacg
    11701 gcgcgcgttt atcgctgctg gttggctgtc tggtggtggt gttatcgctg attatgggcg
    11761 ttattctcgg cctgatcgcc ggttactttg gcggcctggt cgataacatc atcatgcgcg
    11821 tggtcgatat catgctggcg ctgccaagtc tgctgttggc actggtgctg gtggcgattt
    11881 tcggcccgtc gattggtaac gccgcgctgg cgctgacctt cgttgccttg ccgcactatg
    11941 tgcgcttaac acgcgccgcc gtgctggtgg aggtgaatcg cgattacgtt accgcgtctc
    12001 gcgtggcagg tgccggggcg atgcgtcaga tgtttattaa catcttcccg aactgccttg
    12061 cgcctctgat tgttcaggcg tcgctcggtt tctctaacgc cattctcgat atggctgctc
    12121 ttggtttcct cggcatgggg gcgcagccgc caacgccgga gtggggcacc atgctctccg
    12181 acgtgttgca gttcgcgcaa agcgcctggt gggtcgtgac cttcccgggt ctggcgatcc
    12241 tgctgacggt gctggcattt aacctgatgg gtgacggtct gcgtgacgcg ctcgatccca
    12301 aactgaagca gtaagaggtt cgagatggcg ttattaaatg tagataaatt atcggtgcat
    12361 ttcggcgacg aaagcgcacc gttccgcgcc gtagaccgca ttagctacag cgtgaaacag
    12421 ggcgaagtgg tcgggattgt gggtgagtcc ggctccggta agtcggtcag ttcactggcg
    12481 attatggggc tgattgatta tccgggccgc gtgatggcgg aaaagctgga gtttaacggt
    12541 caggatttgc agcgtatctc agaaaaagag cgccgcaatc tggtgggtgc cgaagtggcg
    12601 atgatcttcc aggacccgat gaccagcctt aacccgtgct acaccgttgg tttccagatt
    12661 atggaagcga ttaaggtgca tcagggcggt aataagagca ctcgccgtca gcgggcgatt
    12721 gacctgctga atcaggtcgg tattcccgat ccggcatcgc gtctggatgt ttacccgcat
    12781 cagctttccg gcggcatgag ccagcgcgtg atgattgcga tggccattgc ctgtcggcca
    12841 aaactgctga ttgccgatga accgaccact gcgctggacg tgaccattca ggcgcaaatc
    12901 atcgaactac tgctggagct acagcagaaa gagaacatgg cgctggtgtt aattacccat
    12961 gacctggcgc tggtggcgga agcggcacat aaaatcatcg tgatgtatgc cggtcaggtg
    13021 gtggaaacgg gcgatgcgca cgccatcttc catgcgccgc gtcacccgta tactcaggca
    13081 ttgctgcgtg cgctgccgga atttgctcag gacaaagaac gtctggcatc gttgcctggc
    13141 gttgttcccg gcaagtacga ccgcccgaac ggctgcctgc ttaacccgcg ctgcccctat
    13201 gccactgaca gatgtcgcgc tgaagaaccg gcgctgaata tgctcgctga cgggcgtcag
    13261 tccaaatgcc attacccact tgatgatgcc gggaggccga cactatgagt acgcaagagg
    13321 ccacctcgca acaaccgctg ttgcaggcta tcgacctgaa aaaacattat ccggtaaaga
    13381 aaggtatgtt cgctccggaa cgtctggtga aggcgctgga cggcgtttcg tttaaccttg
    13441 aacgtggcaa aacgctggcg gtagtaggtg aatctggctg cggtaaatcg accctcggtc
    13501 ggttgctgac gatgattgaa acgcccaccg gcggcgagct gtattaccag gggcaggatc
    13561 tgcttaagca cgatccgcag gcgcagaagc tgcgtcggca gaaaatccag attgtcttcc
    13621 agaaccctta cggttcgctg aatccgcgta aaaaagtcgg gcaaattctt gaagagccgc
    13681 tgctgatcaa caccagctta agcaaagaac agcgtcggga aaaagccctg tcgatgatgg
    13741 cgaaagtcgg cctgaaaacc gagcactatg accgctatcc gcatatgttc tccggcggac
    13801 aacgtcagcg tatcgctatt gcccgtggtc tgatgctcga cccggacgtg gtgattgccg
    13861 atgaaccggt ctccgcgctg gacgtctcgg tgcgtgcaca ggtgctcaac ctgatgatgg
    13921 atttgcagca ggaactgggg ctttcttatg tcttcatctc ccacgacctg tcggtggtgg
    13981 agcacattgc tgatgaagtg atggtgatgt acctgggccg ctgcgtggag aagggaacga
    14041 aagaccaaat cttcaataac ccgcgccatc cgtacactca ggcgctactt tccgcgacgc
    14101 cgcgcctgaa tccggacgat cgccgcgagc gcatcaaact caccggtgaa ctgccaagcc
    14161 cgctgaatcc accgccgggc tgcgcgttta acgcccgctg tcgtcggcgc ttcggcccct
    14221 gcacccagtt gcagccgcag ctaaaagact acggcggtca actggtagct tgttttgctg
    14281 ttgatcagga tgaaaatccg cagcgttaac tcacaaaacc ggggccatgc gctccggttt
    14341 actcaatcag cttcaacagt ggcgacaagc atagcaaaat gccgtaaagc agaatcagcc
    14401 aggctttaaa tccgcgaagt ttttccagtt gtgcgacttt atagatcagg aaaaacggaa
    14461 tgaggcacga cacaatgcca tataacgggc ttcccaactg aaagaacacc agcaccgata
    14521 cacgaaacga aacccaaatc gtcaacgtaa tgacgataaa agcgcagatc gccagagtca
    14581 gcacgcgtga gttaattttc ttggtatcaa taattcggct taacagattg agaataatgc
    14641 ctttaatggc ctcgtggaaa ccgagataaa tgccaaagaa tgcggtcagt acggcaaaga
    14701 tattcagcac cgtagaggtg atatgaatga tatgcccagg gatcacctgc gcggccagtg
    14761 ccagtgccga gatattttgt tcaaaggcag aaacggcttc ttcgtggcta attgagaagg
    14821 taaacgaaaa ggcaaaaaac agtatcaccg cgatgagcgt gatataacta attcggtggg
    14881 tacgcagcgc gagccgggtt gccagtactt tatccgcttc ccgtttacga taggcaatat
    14941 tcattgggtt aagtacctga ataaatactg cagaaaagaa gcaaaatgga atggtaagca
    15001 ggacatcgcg aaaaaagacg gaggcttgcg ggaaggcggt tatattggcg aaattccagt
    15061 gcgggatcat cgcaaaaccg aacacgacaa taatccctac tttgaccacc accattggcc
    15121 cagaaatctt aaacagtaat cgttcaccac cagacgcaat cgctaccagt acggcgaaaa
    15181 tagcgacttt ataaagtaga gattgtgaaa gatcggcatc ggttaaaccg aaggttttca
    15241 ggtacgaggc gctgtcgaaa accacggaga gagagtagat aaaaatcccg tggataatca
    15301 tcaaaaagta gataaccccg aggaaaattc cccagttctt ccccaggtaa tgactgataa
    15361 tatcggtgta gtcattacag gaatcgcttt cagaaagggt ttttaaataa atgtcctgca
    15421 ctacccaggt ggcaggataa gcaatgatcg ctgcggtaat aaatacccaa atacccttca
    15481 agccaatttg tactggcatc agcacggttc cggcaccaat cgccatgcct atgcataata
    15541 aaacccagcc gaagtcgtag cgtgtaaacg gcagtttctg atcgtgtttc ggtagtgtgt
    15601 tgtgctgcat aaagtcctgt ccagaaagaa gtcggcctcg caaaaatgaa taaacccagt
    15661 ttctcttgcg caacaggtgg agaagtcgaa tacgcagtat gtaaaaaatg aggcaacaga
    15721 ttcactgggg aggtcgcgag gaggcgcaat tctacaaaat tgcgcatttc actaccagtc
    15781 tggagtcagc ccggatatga actgcaacta ttgcaaataa agaaattaag ctggaaggca
    15841 tcgcgcaatg aggaagattg ccgggtgctg acagtaaaaa gcaatcgtaa aatcagcggc
    15901 aagccggaag ggttccggtg aggcgcaatg ttgcgggggc tttatccctg gtggcattgg
    15961 ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca taacgggggc
    16021 taatcttgaa cctagaccac tcaagaatag ccgcgaaacg ttgtcattac aacacaggcg
    16081 gctatatgac gctcgcagag ctgggcatgg ccttctggca tgatttagtg gctccggtca
    16141 ttgctggtat tcttgccagt atgatcgtga actggctgaa caagcggaag taatgtgtca
    16201 tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga ccaggccgca ggggtgaaac
    16261 tctgcggcct ttttcgtaag aaaaatgcaa cggttgcaag cggataatat tctgcggaat
    16321 gcgcagcaca gagtgaagac gtgacggatt ccggaacaga aaaacagtcg tagaatcagc
    16381 gtcaagccgg aaggattccg gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat
    16441 tggttgctgg agagagaaaa cccccgcacg ttgcaggtat gcacctgaca acatcacggg
    16501 ggccattctt gcatctagac cattcaagaa tagccgcgaa acgttgtcat tacaacacag
    16561 gcggctatat gacgctcgca gagctgggca tggccttctg gcatgattta gcggctccgg
    16621 tcattgctgg cattcttgcc agtatgatcg tgaactggct gaacaagcgg aagtaacgtg
    16681 tcagacgggc gtcaggctac cgtaatggca atttgcgccc ggaccaggcc gcaggggtga
    16741 aactctgcgg cctttttcgt gcttactgcg ggtaaggcac ccagtcgccg ccgttcaggc
    16801 gaacgtacgg tttatcctgg tattgaataa ctactgcatt tgagttctcg gagaccggtg
    16861 ctgtttgtgg caacccactg gtgagttttt tccagtcaac attgtcttcg gtgaaaatct
    16921 tgccatcaag aacgcgaacc accagatcgg agatagccag gaagctgctc ggttggtcga
    16981 tgacaatcgg tgccccctga tgtggtgcct tcatgccgaa gaatttcacc ccaacgggga
    17041 cgtcggtgat agacgggcta gggatatcac gtaggccaga tacctgcatt ctgtcgccct
    17101 tcagcgcgcc accgtgttcc ggcaccacga ccaccatcac tttacgaccc gatttctcca
    17161 gttcagtaaa gaaggcgtcc agttcatcaa agaatttctg cgcccgcgct ttgtaatccg
    17221 ctgttttgct gacgcccgga taatggttgc cgtcatgcag tggaagcgtg ttgtagaacg
    17281 tggcactacg gctgttttta tctttttcgg taacgtccag ccagcggtta agcacggcgg
    17341 tatcgtcata aaccggcgaa ccatcaaagc ccagcaaaat aaccggcaga tttgtttgat
    17401 ccatcaattc gctctgcatg ccgccatttt cgcgaacttc tttcaaaaaa ccgccgaact
    17461 ggccgttatg tcccatcatc aggtgctggg taaagcccag tttcgacagg ttatcaaaca
    17521 gatagcagtc gttatttgcc ggttgataca gattagtgtg cgaagtctgc ccgcagctgg
    17581 cgcgcagtaa acggatcgcc gccgggccac tgtaggaggt ggcggagtta aagttcttga
    17641 actcaatatc gaaatgcgac cacagcggat gcgacatcaa cccggcggct tcaatatccg
    17701 accaggaaag cgaacaaata ttaatcacca gtagttcaaa tggctgagca tcagcgggca
    17761 gcgaagacgg gaagatcgat ttacgtttcg cctccgcgtt atagaaatta ttcagccagg
    17821 cgttaaggtt cgccgttgtt ggcggtgcag tttgtgcggg catatcaccc actaccggtg
    17881 cgccacccgt cgccgcaacg gttgccgctg cgttaccacc cgtcgttgtt acagtggtcg
    17941 tcggttgacc ggctggccac aaggagaaac ttggtcccgc cagggtaagt acgttcagcc
    18001 acagcagaat ggcaaccaca aaaacggtaa tgcgaatcca ttgtgacagg aataaccagg
    18061 ccactaataa aacaaaaatg gccccaatca tctgccagtt aataaagcgt gtgacaaggt
    18121 cgattaaata atcggtactg aaccccgcca cctgcgaacc ctggctcatt atgctttccg
    18181 ggccaggcaa ccaggtgtca tgccagaaca aagcaaagcc gatcggcagg gcaatccagt
    18241 ggcgcaagcg atgcaggctg tagcgcggaa tgggcatcag cagaaacgcg gcaaacacca
    18301 aattgaggag cggatggaag ttaagatatc ccgcccacaa caggccgaac ttaaccagaa
    18361 aatagaagtt ccagccggaa aggccgcgcc agtattgcca gagggaagaa ggcatggcgg
    18421 tattttgcgt aaattgagtc attttttggt tgccctggct ttttccgtgc ggcgtaacgt
    18481 cccagccggt ttaacataac gaggtttagc aaagaacaaa cgtaaggtgt cgcgaatgcg
    18541 tcgcaaagag tgccgcgcca gatagcccag cgggaaaaat atcagtgcgc aaacgacaat
    18601 aatttcaatg atatcgctga tggtcatcat gatgagcgct ccacagcatc atctaacagt
    18661 cgcatgggtt ctggtattcg tcgccagtgg cgaccatcgt gctcggcatt gatgaccggt
    18721 ttagaacttt gtgttaaagg cagcggcatg ccccattgtt ctggggcaag caggcgcatc
    18781 tgcaccagct cggcactgat ttgatcatct tcaaaccaga ccatacggtt tgagaaaatg
    18841 tcgccagtag gcaatgggaa aatatgattc aacgcggtat ccagatcgtt aatccgacag
    18901 aatgagagaa acagcaccag ccgattaccg ccaatggtca tgatatcgcc ggtacggtta
    18961 gggcgacata gcgtcagggc ttgctcaacg cggatacccg gtaccggacg tagggcaacc
    19021 agaacacctt taccgtgggc aggcaaaaga gggttattca tcatgttgtt gacggcatta
    19081 cagaacacat cccacttctg gaaaccacgc agtttgagcg gctgggtcat tgacagcaag
    19141 gtagtgatat cttccggcac atagcgacta aacttctgcc cttgcacgct ttcgatcatc
    19201 gtcagacagc gggagagtgg cgcattccac gggataacca tatttgcacc gcaggccaat
    19261 aacaaacgtt catcggtggc gcgcaggcta gcggtatttt cccgtacgag gattttcatc
    19321 gcactaccgc gctggcgacg cagagtatga atgctgcggg ccagtggctc gatttgcgca
    19381 ttttgttgta aagaaaagac caccgtcgcc gcctgagcgg tacgggcttc attgaacagg
    19441 acttcgttat tgttgaacag ttgccagtgt tccgatagcg gcggcgcacc ttccagtaca
    19501 gcaacattac tcagaatgcg tttttcgtcg ctgcgtggtt ggatctccgc ctcttcgctt
    19561 tgaactaatg tccagatacc attttgttgc tgaacgctaa gctgctgacg ggcgctgacc
    19621 cctttttcgt tgcaccagaa ggcaatatcc agcaaatgtt ggtcgccctg aaaacgcaaa
    19681 ctggcaagac caaaaagtga acggtattcc tcaagcaaca atgaaaattg tttatcgtta
    19741 ttatttccgg gattaattac caaaagcgaa caatggttta acctgctcca tttattcatt
    19801 ttatccaacc atgagcgaag ccgctcggca ggaatgtttt gccatgcgtt atttgcgcaa
    19861 acaagaataa aaaggtaatt atggggatca atagaacact gcaaatcacg ggccaaatag
    19921 tatagacctt tttcatgatt tagcattgaa aataatttta ttttttccgg cccttgagaa
    19981 tcatctaatt gaaagatttt cgccggatcg ctgtccatgc taatgaccgc gacgtgtgcg
    20041 gtttcagcct gggatgcaat tgtttgattc gccagactga tagcatcttc atggcgatcg
    20101 acgttaaacc accagacgcc gcctgctggc atatgtcgca gctcatccca taatgatgag
    20161 ataccgatag agaatacagg gtccacaatg tccctcatgt caccattttg tctgattatc
    20221 agtttactag cgaaagctaa gaaataaacc taccattgaa attaaaactt atcagattta
    20281 gcatgtgaat caatttagca ggtcacaaga cgtgtgattt tagtcttttc gtttttaact
    20341 ttttctgttg cgtaacggaa agtcaaaaag tgagcaaatt cccgtctcgc cgtaaacaat
    20401 aaaccggcga aatgctcacg gttagaattg tctcatgaac ggtacggtta tttcataggg
    20461 atcaagcaaa atgaataaca atgaaccaga tactctgcct gatcccgcga taggctatat
    20521 cttccagaat gatattttgg cgttaaagca ggcattttca ctgcctgata ttgattatgc
    20581 cgatatttcc caacgcgaac agttggccgc ggcattaaaa cgctggccgt tgctggcaga
    20641 gtttgcgcaa caa
//
LOCUS       DAZFKF010000088        20523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFKF010000088 DAZFKF010000000
VERSION     DAZFKF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.088"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(244..990)
                     /gene="cutC"
                     /locus_tag="ACHKA1_003795"
     CDS             complement(244..990)
                     /gene="cutC"
                     /locus_tag="ACHKA1_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO5681386.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(1004..1570)
                     /gene="yecM"
                     /locus_tag="ACHKA1_003796"
     CDS             complement(1004..1570)
                     /gene="yecM"
                     /locus_tag="ACHKA1_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5681387.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1786..3519
                     /gene="argS"
                     /locus_tag="ACHKA1_003797"
     CDS             1786..3519
                     /gene="argS"
                     /locus_tag="ACHKA1_003797"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO5681388.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLATAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3561..3605)
                     /locus_tag="ACHKA1_003798"
     CDS             complement(3561..3605)
                     /locus_tag="ACHKA1_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681389.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            3696..4184
                     /gene="yecT"
                     /locus_tag="ACHKA1_003799"
     CDS             3696..4184
                     /gene="yecT"
                     /locus_tag="ACHKA1_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO5681390.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4306..4698)
                     /gene="flhe"
                     /locus_tag="ACHKA1_003800"
     CDS             complement(4306..4698)
                     /gene="flhe"
                     /locus_tag="ACHKA1_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO5681391.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4698..6776)
                     /gene="flhA"
                     /locus_tag="ACHKA1_003801"
     CDS             complement(4698..6776)
                     /gene="flhA"
                     /locus_tag="ACHKA1_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO5681392.1"
                     /translation="MSNLAAMLRLPTNLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6769..7917)
                     /gene="flhB"
                     /locus_tag="ACHKA1_003802"
     CDS             complement(6769..7917)
                     /gene="flhB"
                     /locus_tag="ACHKA1_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO5681393.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8119..8763)
                     /gene="cheZ"
                     /locus_tag="ACHKA1_003803"
     CDS             complement(8119..8763)
                     /gene="cheZ"
                     /locus_tag="ACHKA1_003803"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO5681394.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRKFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8774..9163)
                     /gene="cheY"
                     /locus_tag="ACHKA1_003804"
     CDS             complement(8774..9163)
                     /gene="cheY"
                     /locus_tag="ACHKA1_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO5681395.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9178..10227)
                     /gene="cheB"
                     /locus_tag="ACHKA1_003805"
     CDS             complement(9178..10227)
                     /gene="cheB"
                     /locus_tag="ACHKA1_003805"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO5681396.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10230..11090)
                     /gene="cheR"
                     /locus_tag="ACHKA1_003806"
     CDS             complement(10230..11090)
                     /gene="cheR"
                     /locus_tag="ACHKA1_003806"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO5681397.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSREWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11109..12710)
                     /gene="tap"
                     /locus_tag="ACHKA1_003807"
     CDS             complement(11109..12710)
                     /gene="tap"
                     /locus_tag="ACHKA1_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO5681398.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQVEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12756..14417)
                     /gene="tar"
                     /locus_tag="ACHKA1_003808"
     CDS             complement(12756..14417)
                     /gene="tar"
                     /locus_tag="ACHKA1_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO5681399.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQSPAQPRLRIAEQDPNWETF"
     gene            complement(14560..15063)
                     /gene="cheW"
                     /locus_tag="ACHKA1_003809"
     CDS             complement(14560..15063)
                     /gene="cheW"
                     /locus_tag="ACHKA1_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO5681400.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15084..17048)
                     /gene="cheA"
                     /locus_tag="ACHKA1_003810"
     CDS             complement(15084..17048)
                     /gene="cheA"
                     /locus_tag="ACHKA1_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO5681401.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFGVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(17053..17979)
                     /gene="motB"
                     /locus_tag="ACHKA1_003811"
     CDS             complement(17053..17979)
                     /gene="motB"
                     /locus_tag="ACHKA1_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO5681402.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGNRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FKTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17976..18863)
                     /gene="motA"
                     /locus_tag="ACHKA1_003812"
     CDS             complement(17976..18863)
                     /gene="motA"
                     /locus_tag="ACHKA1_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO5681403.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18990..19568)
                     /gene="flhC"
                     /locus_tag="ACHKA1_003813"
     CDS             complement(18990..19568)
                     /gene="flhC"
                     /locus_tag="ACHKA1_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO5681404.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19571..19921)
                     /gene="flhD"
                     /locus_tag="ACHKA1_003814"
     CDS             complement(19571..19921)
                     /gene="flhD"
                     /locus_tag="ACHKA1_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO5681405.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4961 a         5618 c         4968 g         4976 t
ORIGIN      
        1 aaggaataat ccagttgtta tttagaatat atatttgtca gttttagcaa tttaaatcac
       61 ataattaatg tgaatatata aaatattttt agcacaacgc tatgtgcact gtcacccgct
      121 caacagatcg cggtaatcgt tcatttttta tactattcag ggataaaaaa aggcccctgt
      181 tgaaattgca ggggcctggt acgagcaagc atcatattgg gcgacatgat gcaacggtaa
      241 aaatcatttg gcctgatggc gttcaatgat tcctttcatt tcagcaaccg ccgccccgtc
      301 tacgacataa cgcgaatact cgtccgcgtg tgcatctgat gacatggaca atccttgatt
      361 acgataacgc atcggtgagg cttgccacgc tcccgcggag ctatggactt ccagcactcc
      421 ggcatcgagg aagtggtgca ggttttctgc acggactcct gctccggcca taatgattgg
      481 agcatcacga tgggcaataa gttccataat ttttgataaa ccttgcagtg cgtctgattt
      541 ttgccctgat gtcagcaccc gggcaatgcc caattccgca agattattga gtgtatttag
      601 agggttagcg cacatatcaa aggcgcgatg aaaagtcact gccagcggac cggcagcagc
      661 cattattttt tccattcgtg gcatatcgac attcccgtca acatcgagaa cgcccgtcac
      721 cagtccagga aaacctaatt cgcggaccgt gcgcacatcc tcaagaatgg cggcaaactc
      781 accgtcgctg tagcaaaaat caccaccgcg tgggcgaatt atcggatgca cagggatcgt
      841 cacccgctgg cgcacggatt tcagtacacc caacgacggc gttaagcccc cctcttttgg
      901 ggctgcgcat aattcaactc tgtctgcgcc gttttgctgc gccgttagtg cacattccat
      961 gctgtaacag caaatttcca gtaatgccat ttttactcct taattacgcc gactgctcgc
     1021 tggcaacgat ctcttcaatg gaccacggat gaaacttaat agttgttttg ccatcggtga
     1081 ccgccagcgt cggattcggt aatcgctcat gctcaccttt tggcgaactg gttttcactg
     1141 agatccccgg taggctgagt ccttcatcag aaagcaatgc cagcgcacgg gcattcagcg
     1201 tttctggatc acccggcaaa acgatttcaa tatgttccca accttcgtgt gggtaacgtt
     1261 tttccccggg ccacggtagc tccacaatag aaaactgcca gtgcgcaacc tgtaccggtt
     1321 catgcaattt aaacagacaa atcggtctgc cattgatcat attttctgac aaaagctcgc
     1381 cgcactgttc aaacccgcga cgccagcgtt cagcggtggc gttttgatgg caacgcaagg
     1441 aaatgtgatc ggcagtcaac ggagtgatat ccaaccccaa acggcgggaa agttcatcta
     1501 atgcgtgggt aaatcgcggt aaatccgatg caatatcctg cagctcgtcg atagattgcc
     1561 agttcgccat aatcactctt cgtctttcag taaaagcgtt aatttaccct gttgccctgc
     1621 gccaaccaac cgctgatttc acgccgcttc tgatgcaata gtgaaaacgg caatacgcca
     1681 cgcgcacgtt gctgacgaaa acagccattt gcagtatact cccgccctaa tttctttaac
     1741 tggtgcgggc aatttttgct cgcttcatca atgtaaggta ttccggtgaa tattcaggct
     1801 cttctctcag aaaaagtccg tcaggccatg attgcggcag gcgcgcctgc ggattgcgaa
     1861 ccgcaggttc gtcagtcagc aaaagttcag ttcggcgact atcaggctaa cggcatgatg
     1921 gcagttgcta aaaaactggg tatggcaccg cgacaattag cagagcaggt gctgactcat
     1981 ctggatctta acggtatcgc cagcaaagtt gagatcgccg gtccaggctt tatcaacatt
     2041 ttccttgatc cggcattcct ggctgaacat gttcagcagg cgctggcgtc cgatcgtctc
     2101 ggtgttgcta cgccagaaaa acagaccatt gtggttgact actctgcgcc aaacgtggcg
     2161 aaagagatgc acgtcggtca cctgcgctct accattattg gtgacgcagc agtgcgtact
     2221 ctggagttcc tcggtcacaa agtgattcgc gcaaaccacg tcggcgactg gggcactcag
     2281 ttcggtatgt tgattgcatg gctggaaaag cagcagcagg aaaacgccgg tgaaatggag
     2341 ctggctgacc ttgaaggttt ctaccgcgat gcgaaaaagc attacgacga agatgaagag
     2401 tttgccgagc gtgcacgtaa ctacgtggta aaactgcaaa gcggtgacga atatttccgc
     2461 gagatgtggc gcaaattggt cgacatcacc atgacgcaga accagatcac ttacgatcgt
     2521 ctcaacgtga cgctgacccg tgatgacgtg atgggcgaaa gcctctacaa cccaatgctg
     2581 ccaggaattg tggcggatct caaagccaaa ggtctggcag tagaaagcga aggggcgacc
     2641 gtcgtattcc ttgatgagtt taaaaacaag gaaggcgaac cgatgggcgt gatcattcag
     2701 aagaaagatg gcggctatct ctacaccacc actgatatcg cctgtgcgaa atatcgttat
     2761 gaaacactgc atgccgatcg cgtgctgtat tacatcgact cccgtcagca tcaacacctg
     2821 atgcaggcat gggcgatcgt ccgtaaagca ggctatgtac cggaatccgt accgctggaa
     2881 caccacatgt tcggcatgat gctgggtaaa gacggcaaac cgttcaaaac ccgcgcgggt
     2941 ggtacagtga aactggccga tctgctggat gaagccctgg aacgtgcacg ccgtctggtg
     3001 gcagaaaaga acccggatat gccagccgac gagctggaaa aactggctaa cgcggttggt
     3061 attggtgcgg tgaaatatgc ggatctctcc aaaaaccgca ccacggacta catcttcgac
     3121 tgggacaaca tgctggcgtt tgagggtaat accgcgccat acatgcagta tgcatacacg
     3181 cgtgtattgt ccgtgttccg taaagcagaa attgacgaag agcaactggc tacagctccg
     3241 gttatcatcc gtgaagatcg tgaagcgcaa ctggcagctc gcctgctgca gtttgaagaa
     3301 accctcaccg tggttgcccg tgaaggcacg ccgcatgtaa tgtgtgctta cctgtacgat
     3361 ctggccggtc tgttctctgg cttctacgag cactgcccga tcctcagcgc agaaaacgaa
     3421 gaagtgcgta acagccgtct aaaactggca caactgacgg cgaagacgct gaagctgggt
     3481 ctggatacgc tgggtattga gactgtagag cgtatgtaat cgatttttcg tgagagtgaa
     3541 gcctgatcag ggttagccga tcaggctttt ttattgccat ctaaatgtat tctgaaaacg
     3601 gacatgccat cgttttctca ctgttgagta agaggccaga agcgtaatat ccaggcccag
     3661 gaaaacgata acggttcaat ttaaggaata ccgcagtgtt taaatttctt gtattaacat
     3721 taggcattat ctcttgccag gcttacgcag aagatacggt tatagtaaat gatcatgaca
     3781 tttcagccat caaagattgt tggcaaaaaa attcagatga tgatactgac attaacgtga
     3841 tcaaatcatg tctgcgacaa gaatacaatc tcgtcgatgc gcaattaaat aaagcttatg
     3901 gtgaagctta tcgttatata gaacaagtgc cacgcacggg tgcaaaaaaa cctgataccg
     3961 aacaacttaa cttgcttaaa aaatcacaac gagcctggct ggattttagg gacaaagaat
     4021 gtgaattaat cctttcaaat gaggatgttc aggatttaag tgacccttat tctgaatcag
     4081 aatggctatc atgtatgatc atacagacca atacgcgaac tcgccagttg cagctatacc
     4141 gtaactctga agatttttat ccaagccctt tgacaagagg ataattcaca tctttttggc
     4201 atgttttgtt gcaagctatt cctgataaat aattgcaaca aaacatcgag cctttttcgc
     4261 tgagttatta aacatgctcg cgagcgcgta atttttattt gtccttcagc gataattcac
     4321 aatcacttca ttccgttgta cctttagcgg tggaattaac cgcccaccgc caggcacttc
     4381 ccagataaag cgcaacggtt ctgctgccgc tataccggaa aaggccacgg tggttccgct
     4441 ctgcccctct aattcgacac aacgagattg cgaacacaag cgaacccgca gtcctgcagg
     4501 tgtcgggccg ataagctgat accgccacgc caccagcgtc atcaatcctg aagcaggttg
     4561 tcgagtagaa agaggcgctg acgacatcga ctcaccgcga tgatttagcg taattccgac
     4621 actacttgcc tgccacatcc cctccccggc ggcttgcacc agcagcggaa acaataatat
     4681 tgctaataag gttctcatca tttaccgcca attgtcgccg tcatgcggat atgtcggtta
     4741 tcagatagtt ccagattcga cagcaccact aactgcggca agctgcggcg cagaaagcga
     4801 gacaataatg gtcgcagcgc gtggttcacc aacaatactg gcggcgcccc caacatctcc
     4861 tgacgggata gcgcttcctg agtttgcgcc agtaaacgat ccgccagccc tggctccagt
     4921 ccgcccccgc cctgcaacgc ctgtagcaac aaacgttcca gcggtgtatc gaggccaata
     4981 acatggactt catctttgcc aggaaaccac tgctgggtaa tcgcccgtcc caacgccacg
     5041 cgcacgacgg cggttaattc atgtggatca ctttggatgg gtgcatgttc cgccagcgtt
     5101 tcgagaatag tgcgcatatc gcgaatcggc actttttcat cgagcagatt ttgcagcact
     5161 ttatgcagtg tggtgagcgt gacgacgcca ggaacgagat cttccgtcag ctttggcatc
     5221 tcctgggcga cgcgatccag cagctgttgc gcctcctgac gaccaaacag ctctgcggca
     5281 tgctggctaa tgaggtggtt aagatgcgtt gctaccaccg tgctggcctc aaccactgtg
     5341 tacccctgaa tctgcgcctg ttcttttagc gcactttcaa tccagatagc attcaggcca
     5401 aatgccggat cgacggtcgc ctcaccaggt aacgtcccgg cagcggtacc agggttaatt
     5461 gccagccagc gccccggata agcatcacca ctgccaatct ctacgccttt catcaaaata
     5521 cgataacgtg ccggttgcag atccatattg tcgcgaatgt gcaccaccgg cggcaagaag
     5581 cccatttcct gagcaaattt cttacgaata ctacgaatac ggcccagcaa ctcgccatct
     5641 tgttggaaat caaccatcgg gatcagtcga taacccactt ccattcccag agaatcttcc
     5701 agttgtacat cgttccacgt cgcttcgaca acggtattat tctctgccat ttttaccggt
     5761 ttgggttcgg caggcgcttt ttgttcgcgt ccgcgtatcc accaggccag cccgagcaat
     5821 ccggcagtga acagcaaaaa taccaggttc ggcattccag gcaccaggcc gagtaaaccg
     5881 agcacggcgg cgcttaacaa cataacgctt gggttactga aaagctgatt caccatctgc
     5941 tcgccaacat cctgatcggt gctgacacgc gtaacgatga cccccgcggc ggtagaaatc
     6001 accagcgccg gaatttgtgc caccagaccg tcgccaatgg tcaatagcgt ataactttcc
     6061 gccgcgtgtc ccatgctcat gccatgttgc agcacgccga ccagcaaccc gccgacaacg
     6121 ttaatgacca tgatgaggat cccggcgatg gcatcgccgc gaacaaactt acttgccccg
     6181 tccattgagc cgtaaaaatc ggcttcctga gtcacttcgg agcggcgttt tttcgcctca
     6241 tcttcaccaa tcaatccggc gttaaggtcg gcgtcaatcg ccatctgctt acccggcata
     6301 ccatcgagaa caaagcgcgc acccacttct gcgatacgtc cggcaccttt ggtaatgacc
     6361 ataaagttga tgatcacgag aatgacgaac accacgatac cgatagcgaa attgccacca
     6421 acgaggaagt gaccgaacgc ttcgaccacc ttcccagccg ccgccgcgcc ggtatgccct
     6481 tccatcaaaa tgatacgggt tgaagccacg ttaagtgcca gacgcaacag cgtggtaaac
     6541 aacagaatgg tcggaaacgc agcaaactca agcgtgcgct gggtaaacat cgccaccagc
     6601 aacaccatga tcgacaaggc aatattgaag gtaaacagca ggtcgagtat gaatgcgggc
     6661 agtggcagca ccatcatcga caagatcaac aggatcaaaa tcggtccggc aaggatctgc
     6721 cattgtgtcg atttcaggtt tgtgggcagg cgcagcatcg cggccagatt actcatgggt
     6781 cggtttctcg ttaataaaat ccagggcttc cggcaccgga agatgagtag gttgtacagg
     6841 gcgctgtcca ccagccagac gccagcgttt cagttgccag acccaggcca gcacttccgc
     6901 caccgcggcg tacagttgcc ccgggatttg ttgaccaatc tccgcatgtc gatacagcgc
     6961 tcgcgccagc ggcggcgctt caagcgtcgg gacgttattt tcagcgccga tttcacgaat
     7021 gcgcagcgcg acaagccctg cacctttagc gaccactttc ggtgcgctca ttttgttttc
     7081 gtcatactgc aacgctaccg aatagtgggt cgggttattg acaatgacat ccgctttcgg
     7141 cacatcggcc atcatccgac gccgtgcggc agctcgctgc atctgacgga tccgtccttt
     7201 aacatggggg tcgccttcgc tttgtttgaa ctcatcacga atatcctgcc gcgacattcg
     7261 cagctttttc aggtggctga agatttggaa aaagacgtca aatcccacca ttggaatgac
     7321 accaagcacc accagcagtg cgcatagccc taccaaatcc atcgcattac ccatggcggt
     7381 aatcggagac tcggccatta agcgcatcat ctgcggccag tgatgccaga gaaaaaaccc
     7441 cgtcacgctg ccaaccagga tggttttcaa aattgcttta agcaactccg cgccagtctg
     7501 tgccgagaac atccgtttta tgcccggtag cgggttgagt ttggaaaact tcggctgcaa
     7561 ggattttccg ctaaatacca gcccccccag cataaccgga gaaatgagcg ccaccagcac
     7621 cacgccgcta atcagcggta gcagcgccag catggcttct ctgatcagca gaataatttg
     7681 cccgaggatc agattcgggt cattgataat actgtgatca aaatgcagcc cagcggagag
     7741 catgcccgac aatcgacggg ccagcgacac accgccaaac cagataacac taacgcccac
     7801 cagcaaaatc agcagtgagg tcagttcacg ggaacgcggg atttgcccct cttcccgcgc
     7861 tttttctagt cggtgaggtg tgggggcttc tgttttgtcg tcgctctcgt cagacacgtc
     7921 gccaatcctg gaaagagtat taagcgtaaa tgatgccaga gcgcaaagcg ttcaatggtt
     7981 tgagtaaggg gcaaaacagg cgggatttag ggcttttgct gccacacatc aagcatagtg
     8041 tgcgtttgtc ggatgcggcg tcatcgcctt atcagaccgc ctgatatgac gtggtcacgc
     8101 cacatcaggc aatacaaatc aaaatccaag actatccaac aaatcgtcca cctgatcctg
     8161 actggctacc acaccggctt tgctggtatc gacctgaggt ccattaagca aactctggtt
     8221 ttcacgtttt ggacgcgact cctgttccgg gatgttttcc aacagcacca tcagcaactg
     8281 gcgttcgatc tcctgaatga catccatcat ccgcttaatg acctgcccgg tgagatcctg
     8341 aaaatcctgc gccatcatga tttccagcag ttgcgcgtta gtaaagctgg tatgcgcggg
     8401 tacatctgcc agaaatttcc gcgtatctgt taccagttca cgggcgtcgg caaggtcaat
     8461 cggatcggca aaccagtcat cccaacgttg ggttaacgct tttgctgatt tctccatttg
     8521 atcctgatgc ggttgtgacg cctcaacact gttcagcgcc cgctccgcag cctgggcggt
     8581 catctgcaca acatagtaca aacgatcgcg cgcatccggg atggcttccg ccgcttcggc
     8641 aatggcctga tccagcccca gttcccgcaa actgtcgcgc agcatacgcg tcaggctgcc
     8701 gatgcgcgca atgatatcgc cagctgaatg ctcgtcagca ggtttgattg atggttgcat
     8761 catagtcgca tcctcacatg cccagtttct caaagatttt gttgagtttt tcctccagcg
     8821 tcgcggcggt aaatggcttc accacatagc cactggcccc cgcttgcgcc gcagcaatga
     8881 tgttctcttt cttcgcttct gcagtcacca ttaacactgg caatgctgac atcgcgccat
     8941 ccgcacgaat tgttttcagc aattccaggc catccatgtt gggcatgttc cagtcggaaa
     9001 taacaaatcc ataaccgcct gcctgcaact tattgagagc gtcgacgcca tcttccgctt
     9061 cctcaacatt attgaatccc agctctttca gcaggttacg cactatgcgt cgcatggtgg
     9121 aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc catttcacac tcctgattta
     9181 aatacgtatc gcctgtccgg cactaatttt tgccagcatt tgctggctta cctggctaag
     9241 atcgaccact tcgcagacac cacccatatt gatggcctca cgcggcatgc cgaacaccac
     9301 gcaacttgct tcgttttgcg caagggtcca tgcccccgcc tgacgcatcg ccaacattcc
     9361 tgccgcgccg tcgttgccca taccggtcag gatcacccca accgcattac gccccgcctg
     9421 tttggcgaca gaatggaaca acacatctac cgaaggccga tgacggttaa ccgccgggcc
     9481 atcgtgaatt ttgatttgat aatttgcgcc actacgcgcc agctccatat gccgatcgcc
     9541 cggcgcgata taggcatgcc ccggcaagac acgttctccg tcttcggctt ctttaacccc
     9601 gatctggcaa agcttattaa gtctgtcggc aaaagagcgg gtgaaaccgg gcggcatatg
     9661 ctgggtaatt aacagtgccg ggctggaaag cggcaacggt tgcagtacgt gacgaattgc
     9721 ctcagttcca cccgttgaag caccaatcgc aatcagtttt tcagaactca acaacggccc
     9781 tgccttcagc gttgtcggtg ccgacaatgg cttatgtgct gcaaggctcg cctttgctgc
     9841 tgtacgcacc ttttcagcaa tcatttcgct gtacgccagc attccttcgc gaatacccag
     9901 ttgcggtttg gtgacaaaat ctattgcccc cagctccagc gcgcgcagcg tgacttctga
     9961 tcctttgccg gtcagggaag aaaccatcac aacgggcatt ggacgcaaac gcattaattt
    10021 ttcgaggaaa tccagtccgt ccatccgcgg catttcaaca tccagcgtca gcacatcggg
    10081 attgaatttc ttaatcaagt cacgcgcgac cagcggatca ggcgcggtcg ccaccatttc
    10141 catgtcgcta tggctgttga tgatttctgt catgatctgg cgcatcagtg ccgaatcatc
    10201 gacagataac accctgattt tgctcatcgt taatccttac ttagcgcata caccgtctga
    10261 ccacgcagcg tgaagcggcg ctcaaggtgg ctaaagtttt cagagtgacc cgcaaacagc
    10321 aatccgtcgg gtttaaggag cggaacaaag cggcgcaaaa tctcctgctg ggtagtttga
    10381 tcgaagtaga tcatgacgtt acgacagaag atcgcatcaa acggccccgg cacggtgtac
    10441 tgtttcgcca gtagattcag cggggcaaaa tcaacatagt tcgccagctc ctgacgcacg
    10501 cgtaccagcc cttcatgcgg tcccgtccct cgcatgaaat accgttgcag ttgctgcggc
    10561 gtcaggtttt tcaactcttc atggcgatag ataccgcttc tggctttttc cagcacttcg
    10621 gtgtcgatat cactggcaaa cactttccag cgtccgggcg cggtgcccaa tgtgtcagcc
    10681 agcgtcatcg caatgctgta cggctcttcg ccggtcgaag ccgccgcgct ccatacgcga
    10741 tactcgccag aacggcgacg tgcgtgatcc gcgagcagag ggaaatgatg tgcctcacgg
    10801 aaaaatgccg tcagattcgt ggtcagcgaa ttgataaacg cctgccactc acggctgtgc
    10861 tgattagatt ccagcaagtt caagtaatga ccgaaatccg tcagtcccag cgaacgcaaa
    10921 cgacgaacca gtcggttgta aaccatgtcg cgtttatggt cagccagaac gatcccggct
    10981 cgttgataga tcaattgact tatccgccga aaatgtgcgt cggaaagcgc caggcgctcg
    11041 gtcatctgta acaataaaga cgtttgccca cagggcagag atgaagtcat agcgccttct
    11101 caatcacttc aggataccac tggcgcaatt tgtaactgcg ccgactcatg tcgtgctact
    11161 tcatgttctt caagggtaaa taccgccaca cgcgacgaaa gatggtcggc ctgattcgcc
    11221 agttgttccg ttgccaccgc cgcttcttct accagcgagg cgttctgctg agtcacctga
    11281 tccatctggc tgacagcctg tgcaacctgc tcaatccccc gccgttgttc ttccgacgcc
    11341 gaagcaattt ctcccataat gtcgttcacg cgggtcaccg aactgacaat atcggtcatg
    11401 gtcgcggcgg cgttattcac cagtttcgaa ccctgctgga cacgattgac tgactcttcg
    11461 atcagccctt tgatctcttt tgctgcctgc gcgctacggc tggcaagatt gcgcacttca
    11521 cctgccacta ccgcaaaacc acgcccctgc tctccggcgc gagccgcttc cactgccgca
    11581 ttcagggcca gaatattggt ctggaaagca attccgtcga taacgctgat aatgtcgcca
    11641 attttttgcg agctggtggc gatctcctgc atggtgtgag tcatggtact gacctgaaca
    11701 ccgcccgcct gcgccgttgt cgcagcattt tttgccagtt ccgacgcctg tcgtgcgtta
    11761 tcggcgtttt gccctaccgt ggcggttaat tgctccatac tggcagccgt ttgtgccagc
    11821 gatgccgcct gctgttcggt acgacttgag agatcgttat tgcctgcgac aatctctgcg
    11881 atcccaatgt gcatctcatg gcttccctta cgcacatcac tcaccgtccc acgcaaagcc
    11941 tgctgcatgg tcttcaggct ggcaaaaatg gcggtgatct cattacgacc atataccgca
    12001 atcggacgcg ccagattacc cgcagcaatg ctgtcaaaat ggctaccgat aatggccagt
    12061 ggttgaacaa tcatcttgcg cgtccaccac agcgcactgc tgatgtagat cgccgcaaca
    12121 ataatcatgc tgataaacac cagcgccgaa atctgatagt tacgctggct ttgcgcactg
    12181 gcggcttcca gcacatggtt gatctccacc tgccaggcct caaagttaac gtcaaacgca
    12241 ttctgtgatc cctgcaccgg cgcagtgagg aaatccgaaa gttgattact ttcaagccag
    12301 gtcgcctgat gttcgagatc gttgtgccag cgggcaaaac ttttctccgt ctctttttgc
    12361 aatgccctga cgtgctcgtt gcccgcagtc atcgccataa aacttttaaa cagcgtggtg
    12421 gattgcgtca gactggcgcg cgccgtcgtc atcaacgttt taatgtcatc cgccggatag
    12481 ctaagtgccg tcagagtgcc cgctttgttc agcgcggtac tggcctgtaa cattaccgcc
    12541 cgagtttgcg ctaatgccgc acgttgctga ttgctctgct cgacctgatt cagccgttgc
    12601 aaatcgtcgc gaaatgccca aaaagacatg ccgttactgc caatctgcaa gatcccgcag
    12661 agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac gattaaacat caacgctccc
    12721 catcaggcgg caatgaccgc gttagtaaat actcgtcaaa atgtttccca gtttggatct
    12781 tgttcagcaa ttcgcagtcg tggctgtgcc ggtgattgct cactggcagg acgggatggt
    12841 gtttgcggtt tattggtgag tgggctggct gccagacgaa acgcggaaac cgcttgcgtt
    12901 aaacgactag cctgttcttc cagcgcagct gcagcggcag ctgattcctg caccagcgat
    12961 gcgttctgtt gcgtgacgcg atccatttcc gaaaccgcca atgcgacttg atcgatgcca
    13021 cggctctgtt catccgatgc cgatgcaatc tcgcccataa tgtcagtcac gcgagtgaca
    13081 gcattgacga tattgttcat tgtttccccg gcgctttcga ccagcaccga accggtatca
    13141 acgcgcgaga cggagtcttc aatgagagct ttgatctctt ttgccgcctg ggcgctacga
    13201 ctggcaagat tacgcacttc gcccgccacc acggcaaaac cacggccctg ttcacccgct
    13261 cgcgcggctt caaccgcggc attcagcgcg aggatattag tctggaaggc aataccgtcg
    13321 attacgctga taatgtcggc gattttcttc gaactatcgg cgatctcatg catcgttttc
    13381 actacgccat ccaccacttt gccgccgtgc tgggcggtgt cggaggcact ttgcgccagt
    13441 tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg tcgcggtgag ctgctccatg
    13501 ctggcggcag tttcttccag cgcggatgcc tgctgttccg tacgggagga aagatcggtg
    13561 ttacccgccg caatttcacg ggtaccggca tagatggcat ctgaaccttc gcggacatga
    13621 gtgacggtgt cagtcaaaga gcgttgcata tgtgaaacgc tctgcgccag gtcgcccatt
    13681 tcactgcgcc cgtcaatggt cagggtattc gccaggttac caccggcgat ttcgcgaatg
    13741 tgagcaataa tttttgccag cggtgtaagc aacatacggc gaatgccata ccacgccacc
    13801 agcagaatca ataccaccac cagcgcgata accgccagtt gccactgggc aaatcggtaa
    13861 tcatctgcgt tgtcagtgac gatatcgcga tacagttttt cactgctgag ggcgtactga
    13921 gcaaacgctt cgcccattgc attttgcatt ccctgggttg gctgagcgaa ataagctcca
    13981 gtattgccat aatcaagata atcaatcagt tcagttaacg ctgtgtaata gtttttatat
    14041 ttttcatcaa tattacgact ggtagcgacc atttcaggta acggtgccat gcttttgaat
    14101 tttttataat gagtcgcggc ctgggccaat gttttcctgg cgctatcgag caattcaact
    14161 ttggcgttac tttgttgatt ggaggaatcc atcatcatcc gcactgctga acgactcagg
    14221 ttaatgcgcg tttgcagcat taaatcccag gttgacgtca gctcgccctg ctgttcccgt
    14281 aattgattgg aaaccacaaa actcttctgg ctatggtgaa gggaagaaaa aaacagactg
    14341 ccggaaataa gctgtaacag tgcgaatacc cccagcacca ttaccaacag cgtgactacg
    14401 cggatacggt taatcataag gcaccttcct gaaaacaagt tgatctcgtt atcggcaagg
    14461 aagggggaaa ctttattgct gatgccaccc gccgcgaaat tgaaataaaa cccgatgcgc
    14521 agatcatcgg gttcatttca attgaggaaa tcaggagaat tacgccactt ctgacgccgc
    14581 gctatctaac agcgccatct cttcgctgtt cagcagtttt tcgatgttca ccagaatcaa
    14641 catacggtcg cccagtgcgc ccagtccagt gagatattct gttgaaagcg tcacggcaaa
    14701 ctctggtgcc ggacgaattt gctccgccgt caatgaaagc acgtctgaaa cgccgtcaac
    14761 cacgatgccg accacccgct gtccgagatt caggacgata actaccgtgt tgtcgttata
    14821 gtccacatcc acctggctga acttaattcg taagtcaaca atcggcacaa taacaccgcg
    14881 cagattcgtg acgcctttga taaacgccgg cgtgttcgca atccgtgtta cctgatcgta
    14941 gccacggatc tcctgcactt tcaggatatc aataccgtac tcttcatcac caagggtaaa
    15001 taccagaaat tcctggcctg acggctcgct ggccagcttt gttacattcg tcataccggt
    15061 catattgtta cctttttact cattcaggcg gcggtgttcg ccatacgttg ttcgcggttt
    15121 atcgcctgca aggcggagac atcaacaatc agtgccacgc tgccgtcgcc aagaatggtc
    15181 gcagcagaaa tgccggggac tttgcgatag ttactttcaa ggtttttaac cacaacctgg
    15241 tgttgaccaa ttaattgatc caccagcaag gcgtagcggc gaccgccact ttgtaagatc
    15301 accacaattc cctgggtagc ttcggttttc gcgcccgcga cgttgaacac tttccacagt
    15361 tcgacgatgg gcagatattc accccgcact tccagcaccc gctcgccgcc ggccagtgga
    15421 tggagatcgg cttcacgggg ttgcagtgat tccataacag cattcagcgg caaaatgaaa
    15481 acttcatccg caacgcgtac ggacatgccg tcgaggatgg ccagcgtcag cggcagtaaa
    15541 atgcggatcg tcgtgccagt accctgcttc gactggattt cgacatgacc gcccatctcc
    15601 tggatattac gtttaacgac gtccatgccg acgccgcgcc cggagacgtc ggtgacctgc
    15661 tctgccgtgg agaagcctgg tgcaaatatc agcatcgcga cttcgtcgtc gctcatgttt
    15721 tcgctgacag tcaaaccttg cgaggccgct tttgccagaa ttcgctcacg gtttagcccc
    15781 gccccatcgt cggtcacttc aatgcaaatg ttgccgccct gatgttcggc agacagaatt
    15841 aaatttccga cgctgttttt acctgcggcg agccgttttt ctggcagttc aataccgtga
    15901 tcgaggctat tgcgtaccag gtgggtcagc gggtcgataa tgcgttctat caggctcttg
    15961 tcgagctcgg tggaactgcc caccagcgtc agttctacct gcttgccgag ttttcccgcc
    16021 agatcacgca ccagccgggg atagcgacta aagacatatt ccatcggcat catgcgaatc
    16081 gacatcactg attcctgcaa atcacgggcg ttacgttgta actgccccat gctggtaatc
    16141 aaatcaccat gattaaccgg gtccagttcg ctggaacgct gggcaagcat ggactgggtg
    16201 ataaccagct cgccgacgag gttaattaat tgatcaacct tttctaccgc tacacggatg
    16261 ctggtggatt cactgctacg cgtcgttttt tcccgctcca cgcgaccggt tggcgcttgt
    16321 tcggctgcca gtttaagcac tggtggggtg gatatttttg gcgagacttc tactgtttca
    16381 aaggtaatct gatcggcttc aatcacaaaa cagagtaccg ctgtgatgtc atcttcggcg
    16441 atatcgcccg gtaatattgc cgagagagaa tccgcccctt tcaccacgtc agttaacgtt
    16501 gtcagatgcc ccagctcttc ttccagcagg tcgacttcgc cggccttcag gcgcgaaagg
    16561 ataattcgtc gcggcaactg actgcgactc tgctcatctt gcggttcact tttggcaacc
    16621 acacttaatc gggtcactgc ggatggcgtt tcgcctttcg cttctaatgc cagttgacgc
    16681 aaggcctggc agatataatc gaagctggcg gcatccggct cttgcgactg tttataagcg
    16741 tcgagctgtt cttgcatgat gtccttcgtt tccaaaaaca gattgataat gtcggtgttg
    16801 agttgcatct cacctcgtct ggcttcatcg agcaggtttt ccatcagatg cgtggtttcc
    16861 tgcaaaacgc cgaagccaaa agttcctgcc cctcctttga tcgagtgggc agcccgaaag
    16921 atggcattca attgttcggc atctggcgct tccggctgca aaaccagcag atgctgctcc
    16981 atatcagcca acagttcgtc cgcttcatca aaaaatgtct gataaaaatc gcttatatcc
    17041 atgctcacgc tgtcacctcg gttcggctga tggcattgtg ggaacactga cctgtggtgc
    17101 aacctcaggt ttttccaggg cgcttactgg ctcattctgg ctttcggcgt tttcatgcaa
    17161 aatggcctgt tcggcttgtt tgttcagaac cagcaggctg atgcgacggt tgacggcatc
    17221 atcaggtccg cgatcgctta agcgcatcgt tgccgccatg ccgacgacac gtaacacttt
    17281 gccgctatcc aacccgccga ccatcagttc gcggcgggat gcattggccc gatcggcaga
    17341 aagctcccag ttgctgtaac ctttctcacc gctggcgtag ggaaaatcat cggtatgacc
    17401 tgaaaggcta atacggttgg gaataccgtt cagtacaggc gcgatggcgc gcagaatgtc
    17461 gcgcatatag ggttcgacat cggcactgcc ggttttaaac atcgggcgat tctggctatc
    17521 gatgatctga atacgtagac cttcctggac cagatcgatt ttgagatgtg gacgtaacgc
    17581 ccgcagtttc ggatcggact ctatcaactg gtcgagatca ccccgcaatt tccgcaatcg
    17641 actttgctcc atgcgttttt tcaactcttc gatgttcggc tgcttattca cttccccctg
    17701 gctttgggtg taatcatcac caccgccggg gattgggctt tcactattag aaatgcgatt
    17761 gccgcccgta accgctgtcg ccagtggagt ccggaagtac tccgcaatct gaatcaactc
    17821 ttttgggctg gagatggaga tcagccacat caccagaaaa aaggccatca tcgcagtcat
    17881 aaagtcggca taagcaatct tccacgatcc atgtgctgcc ccgtggcttt tggctttgcg
    17941 tcgtttgacg acaataatcg gatgcgcttg attcttcatg cttcctcggt tgtcgtctgt
    18001 tgttgcggat ttttcaccgc acgcacatgc tcttccagtt caataaacga cggacgttcg
    18061 ctggaataga gcgttttgcg accaaactca acggcgatag gcggtgcgta accgttcaga
    18121 ttagaaagca gtgtgacttt gacgcactgc atcattttgc tggtttcggc gcttttctga
    18181 cgtaaaacac tcgctaatgg ggaaataaat ccgtaagcca ataaaatgcc gaggaaagtc
    18241 cccaccatcg catgtgcgat aagcgccccc agctcggcgg caggacgatc ggctgaacct
    18301 aacgcgtgaa cgacccccat tacagccgcg acaataccaa acgccggaag tgagtccccg
    18361 accagcgcca gactgttcgc cgggacttct gcctcgcttt cgtgcgtctc aatctcttca
    18421 tccatcaggg cttcgatttc gaaggtgttc atgtgaccgc tgataatcag gcgcagataa
    18481 tcgacgataa aatcaagcat gacgctatcc gcgaggatgc gtgggtagct ggcgaagatc
    18541 tcgctctcac ggggattttc aatatcacgt tccagtgaaa acatccccat ctgccgcgat
    18601 ttcgccatca accgataaag cagagccagc agatccatat acattgcttt ggtgtatttg
    18661 gagcgacgaa acagcaacgg cagcgctttc agcgtgcctt taatcgcttt gccattattg
    18721 ccgacgataa acgacccaat ccctgcaccg gcaataatca ccagttcagc gggttgatag
    18781 agtgctccaa ggcttccacc ggtcatcaaa taaccgccga aaactgtacc gagaacaacc
    18841 aggtaaccta ataagataag cacgacatca tccttccact gttgaccatg acaggatgtt
    18901 cagtcgtcag gcgttaacgc gcgattgggg caaaaaaaag cagcggtacg tcgttaccgc
    18961 tgctggaatg ttgcgccaca tcgtatcagt taaacagcct gtactctctg ttcatccagc
    19021 agttgtggga taatatcggc aggattctgg gaaagtttac gtctttttac tgcccgggat
    19081 ggcggttgac ataagctgca ggcaaagctg ccaacaggct ggtgagcgtg ggtaataaaa
    19141 ttgccgccgc agcagttgca gctggaaagt tgcagtaatc cactttcaac aaaccgcacc
    19201 aatgtccagg cacgggttaa tgccagcagc ggtccttctt ctgcttgcgg gcactgttca
    19261 aggtataaac ggtaggcttt gatcaccgca tcgacgccat tacacaaacc ggttttcagt
    19321 aaaaactgcc atgcattaca gaacatcgaa gcatgaacgt tttgttccca ggtcataaac
    19381 cagtcggttg agaatggcag catgcctttc ggcggtgggc ttccgcgcag ttctttataa
    19441 agttttatca ggcgtccgcg acttaactgt gtttcgcttt ccagcatctg caaacgagcg
    19501 cccagggtga tcaattccat tgccagctga atatcccgcg cttcctgaac aatgcttttt
    19561 tcactcatga tcaggccctt ttcttgcgca gcgcttcttc aggctgatta acatcattca
    19621 gcaagcgtgt tgagagcatg atgccggtat gaatttgctg gagatcgtca acgcgggaat
    19681 cttgcgtcaa ctgagtaatc gtctggtggc tgtcaaaacg gaagtgacaa accagctgat
    19741 tggtttctgc cagcttaacc atttgcggaa gagtcagtgc cgctaatgtt gtcgccattt
    19801 cttcatttat gccgagacga aacatagcgg acgctttgtc ctgaacaatc agacgctgtg
    19861 caagtagtaa atatgacaag ttgatgtcat aaatgtgttt cagcaactcg gaggtatgca
    19921 ttattcccac ccagaataac caactttatt tttatgcggt ttcaccgcac cccgtgatgt
    19981 cgccgggaag ccccggtaaa aaataattag cattagaata gttgcgataa gctgcaataa
    20041 gcagagccac ctttttggtt taataagtcc ttacaaatag aagggggtct ttacacttat
    20101 ctaagatttt tcctaaatcg acgcaactgt actcgtcact acacgcacat acaacggagg
    20161 ggggctgcga ttttcaataa tgtgtgatgc agatcacaca aaacactcaa ttacttaaca
    20221 taaatgcata agtcatactt ttgttttggg tgtatttcaa tctgttaaaa agtttttcgc
    20281 tacgctagca agcaaaaatg aaacaggaat aatcgaaatg ggatgttgcg cacagttaaa
    20341 ataactcacc gtaaataatc atctgctata aataatcact ttcatgcaat accagataag
    20401 ctatttttaa acagacactt accgcacaac aaactaatta aatacaaaga aaggcaacaa
    20461 tcaacttaat gttaataaaa tgttttgcat atattaacca cttgtttaat gcggggaatc
    20521 agt
//
LOCUS       DAZFKF010000089        20343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFKF010000089 DAZFKF010000000
VERSION     DAZFKF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.089"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            208..1833
                     /locus_tag="ACHKA1_003815"
     CDS             208..1833
                     /locus_tag="ACHKA1_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681406.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWTDVVDVIRSPVAQDSHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGSS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            1830..3098
                     /locus_tag="ACHKA1_003816"
     CDS             1830..3098
                     /locus_tag="ACHKA1_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5681407.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            3113..3391
                     /locus_tag="ACHKA1_003817"
     CDS             3113..3391
                     /locus_tag="ACHKA1_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681408.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            3397..4014
                     /locus_tag="ACHKA1_003818"
     CDS             3397..4014
                     /locus_tag="ACHKA1_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681409.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            4105..4839
                     /locus_tag="ACHKA1_003819"
     CDS             4105..4839
                     /locus_tag="ACHKA1_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5681410.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGNHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKNSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGITAGIGLKF"
     gene            5070..5210
                     /locus_tag="ACHKA1_003820"
     CDS             5070..5210
                     /locus_tag="ACHKA1_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5681411.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            5267..5668
                     /locus_tag="ACHKA1_003821"
     CDS             5267..5668
                     /locus_tag="ACHKA1_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681412.1"
                     /translation="MAPVSGERMNDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            5763..6419
                     /locus_tag="ACHKA1_003822"
     CDS             5763..6419
                     /locus_tag="ACHKA1_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681413.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            6422..6868
                     /locus_tag="ACHKA1_003823"
     CDS             6422..6868
                     /locus_tag="ACHKA1_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681414.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNPQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            6878..7150
                     /locus_tag="ACHKA1_003824"
     CDS             6878..7150
                     /locus_tag="ACHKA1_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFO5681415.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRRTEIEASNKQLAAQQKAQ
                     KKQNIGTGIGTGATIGATVGSAVPVVGTAVGAVAGAVIGGIAGALF"
     gene            7161..8426
                     /locus_tag="ACHKA1_003825"
     CDS             7161..8426
                     /locus_tag="ACHKA1_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681416.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYTLQEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMLARDQPVMAALGKAIDAGDHDAASHLFGKLSDANPLKMMSTVGYAAKA
                     GQAVNNLQKIFDDKPDRAIASLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQ
                     PHQQKIQQQKLQQQVINWAGDDPDKLSFARNVAARQPEMLEPQNQKLLENGYANFLRI
                     KKARGEQARDESASSASQFIRGLKQNYAQ"
     gene            8496..16886
                     /locus_tag="ACHKA1_003826"
     CDS             8496..16886
                     /locus_tag="ACHKA1_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO5681417.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKATGKAVSDGAEATDEWLTGK
                     MSPGAVRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVAGGVAKKVGDVTLRKMLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGAINAAQGGYSRYQENTALRETA
                     GMDVSPWEGVADATIEGAAFGAAMGAPSGAVAGYRGRRQAAEETAMRDAETVQQDDAA
                     PQPESVDPVAQQRESMQGMNREQLLEQYADADMATEGDVSAAHRREAASQLLNELDEQ
                     AKRQAVMNELKAKPRSELLEEYRRLSQKEERTETEEQQFQAIREVIRPQQEVTPEAQS
                     QPENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMG
                     SDVFRNAAATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIADADPME
                     QPAFMRDPRFRGFTGDDTEVQARLARGNAPTAEELVRSQMAEGDAGPTAQELTERPRL
                     PAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQAPAPENAAGR
                     QGETLEGEMVRRGLPSPDAQNATAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPEL
                     AQQAEVRRQAGGNRDIPQPETIAPESETTVSTDREATVRGGEVRGKKIEDFGEEIKGA
                     AKHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDI
                     PAKTKHNTAGWGESIKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLP
                     PSQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLAKAK
                     VWFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSAS
                     DARKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGNVSPEQFSDAFGFR
                     GVQFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAA
                     HYEPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSNDGKITSGGDFMTEAQRVRR
                     IFKDGRYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAA
                     RAFERYVQDKARMAGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIRDAFDHLFST
                     LKTRETDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVVRGVMSGIKDS
                     DLKVRVVKSQKEAEALAGELFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHE
                     IIHHAMEHVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLA
                     HMAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYT
                     PREDGVGASSQYSRSGKPDPFKADAYDAKQFASEVRRIAGLQSVSRREMLRVGDTPRV
                     LRALGAPDLELVMPASVVHKATRPEIREHFVSVETLADLPQLLTDPVVVMRSKTESDA
                     LVSLIQATDTKGNPVIVAIHTHVKGDGFAEINKIASAYGKDKRSALQDQLDTSLLYLN
                     KEKAAEWLHAVGLQLPEANTINGSSGFKILHPEDIRKGPYYSRTSSLTPEDTIASRFV
                     RQMQDKFQVLKAVQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAK
                     LLADYKIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSG
                     KQAQYDRLAGIVDDMLARRRELIREAGLEESGVVDAWQNAYRYYVPLKGQDVDGVVSL
                     PRTGKGFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDN
                     PDKDYWQVFTDDRPDTMRVIAERKDQETGETIREVVERPVAMAMMADRYFTTKKNGKT
                     YYIKLHDPRLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQT
                     AVMNLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGTQWQKVW
                     KEFVEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAV
                     ENALRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTA
                     NLVRTLGHLNGEGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNW
                     YDKVPSHVKERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASR
                     AAGNVVGGVLGAFSPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQEN
                     MPFGTPKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRPGAVDITPESLKYWVDYISG
                     GTGRFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHA
                     ELKSLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQCDAIYADSTLSLAQQSAM
                     VKSVERDMKIAVDRFNREYNKKVGVE"
     gene            17251..18249
                     /locus_tag="ACHKA1_003827"
     CDS             17251..18249
                     /locus_tag="ACHKA1_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFO5681418.1"
                     /translation="MSKKFTKTLLSSSLVGLLVVSAEGFAQVSVYPKGSQYSVLVDDE
                     NKPNEFGSKFKVYYSKSSHRGVVRYVQNGQDNVLFHFGNDGNIFVLSKDGQIGYTVHE
                     PVLKDFARMAEGLKSDGSKNHKLDEAEVQRIYDKIYNMSNTVVNPALKNQVGELSGRV
                     DKIDLAAGAIATDVASKTTVGVNSDGTLTSADGATKTIAVNDGLVALSGRTDRIDAAV
                     GSVDRRVTKNTQAIQSNTRQLQEHNARLNSQQRQIRENHEEMKRAAAQSAALAGLFQP
                     YSVGKFNATAALGGYSDKQAVAVGVGYRFNEQTAAKAGVAASDGDVSYNVGVNFEF"
     gene            18336..18809
                     /locus_tag="ACHKA1_003828"
     CDS             18336..18809
                     /locus_tag="ACHKA1_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681419.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRIILAREFFTPENRKSVPAHD
                     ESWVIICDALLGRLMCCMFQGRRYLQIDAEDVTGRLETYRKIRNGEWVHNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGINTKTVNTFLYMGLGKNGLKYRSVKHLVGRA"
     gene            19180..>20343
                     /locus_tag="ACHKA1_003829"
     CDS             19180..>20343
                     /locus_tag="ACHKA1_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO5681420.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDNSGTVVPAVAGGDIVWKYSQGSGE
                     GTLSQGGQVWKMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLTFNGDYT"
BASE COUNT         5235 a         4408 c         5995 g         4705 t
ORIGIN      
        1 gtgctacgtt atatggcgaa taaagactgc cacagggaag tttgcagaaa cagtgatgcc
       61 gatcgtgttc ctgacgctct tctctgataa gagtaatttt tcagatttca ggaagtctgt
      121 attctgcaca ggcgcagttg tatgccgcct ttaaactctc aattcagtga ctcacctgcc
      181 attcaaattt tcggatacca gacaaccatg ccttatatcg atataaccac tatgcgcggg
      241 atgatgccag gcgttattgc atctatgctg ccggatcatt ctgctgtact ggcagaaaac
      301 tgtcattttc gctatggagt gatcacgcct gaacaccaga tgtcagaggc tgagaaaaca
      361 ttcgcgatta agccgaaaac catttttcat taccgtgacg atttctggtt tgcatggacg
      421 gatgtggtgg atgtgatccg cagtccggtc gctcaggact cccacgggcg tatttactac
      481 actgacgggc gttttcctaa agtgacggat gcgaccattg ccacaaaagg ggacgggaat
      541 cacccgacat catcgtatcg tctggggatc cccgcgccga cgacagcacc tgtctgtact
      601 gttcagcagg gcggtgatgt ttctgacgat aacccgaatg atgacgaaac ccggttttat
      661 acggaaacct ttgtctcaga ttatggtgaa gaaggtccgc caggtccggc gtctctggag
      721 gtaacactcc gtactccggg gactgcggta cagctgacgc tgtctccggt gccattgcag
      781 aatgccagta ttaaacgccg ccggatttat cgctctgcat caggtggagg agaagcggat
      841 tttttacttg tggctgaact ggatgcatcc gtgctcagtt acacggacaa aataccgggg
      901 aaaaaccttg gaccttctct ggcgacatgg gattacctgc cgccgccaga gaatatgaca
      961 ggcctttgcc tgatggctaa tggtattgcc gccgggtttg ccggtaatga agtgatgttt
     1021 tcggaagcgt atctgccgta tgcatggccg gaagtgaatc gtcacacgac ggcagaagat
     1081 attgtggcta tctgtccgct gggaacgtca ctggtggtgg cgacaaaggg ggagccctat
     1141 ctgttcagtg gggtatcgcc ttccacaatt tctggctcca gaattccttc catgcaggca
     1201 tgcctgagcc gaagaagtat ggtggcgatg gagggattcg tactctatgc tgggacaaac
     1261 ggtctggtat ctgttgatgt aaacggtaat acagcactgg caacggaaaa gattatttca
     1321 cctgaacagt ggcagagtca gtttaacccg gcgtccattg tggcttattc ctggcgtggt
     1381 gagtacattg cctgttacac gaaaccggat ggtaagcagg atgtgtttgt attcagtccg
     1441 gtgaacatgg atatccgtta tctcagtaca ccgtttgact gcgcatgggt tgatctcgcg
     1501 aaagatatga tgcgcgtggt gacaggagac aaaatgtcag tgcttgccgg gagctctctg
     1561 ccctccacga taaggtggca ttcaaaaatt ttttcattac ctgaaagaac ctctttttcc
     1621 tgtatcaggg tgaaatctcc ggcgcctgag cgggtgggga tcaccattat ggctgatgat
     1681 gttcctgtga ttcattttgc gccgggtacg tttaagggaa gtgtggtgag acttccggca
     1741 gcaaccgggc aaaactggca ggtgatggta tccggattcg ggcaggtgga acgaataacc
     1801 ctgagtacat cgatgtcgga gatgccggta tgaccagaaa accgtggcgt gcggggaagg
     1861 atttatccac agttgtggag aacatggaaa ttggcaccgg gcagcgtggt gacggacgcc
     1921 acgcatttgt gacccgtgag gaactggttg gtcttaaact cgcccggcgt cgaacatcgg
     1981 gtggtgcctc atatgcactg aatccgggta ttgagattga cagtacttta atgactgttg
     2041 attttcccac aaaaccgctg aattttaagg cgacaggagg atttggctcg gttcttcttg
     2101 aatgggatat gcctaattat cgcggacatt cactgactga aatctggcgg ggtacggagg
     2161 atgaccttgc tgatgcagtg ctggttgcca cgacgccggg gcaggtttac ggcgatccgg
     2221 ttgaccctgg ctggtcggga ttttactgga tacgttttgt taacgcggca ggagtgaaag
     2281 gtccatggaa tgctgaaaaa ggcactcagg cacaaacaca gatcggcgtg aaggccatca
     2341 ttgaccagat ccgcgatgag gctgcaaagt cgccggttgt gtccgagctg cgtaaagaaa
     2401 taaaaaacgc gcaggggcag gctgtaaagg atgctgcaat taagacaacc gaagttgtgg
     2461 ggactctcag ggaagaaacg acaagaacga ttggtggtat tgaaacccgc attagcacac
     2521 tggattcatc aaccagtgaa tcgcttaatg aggtcgacaa gcgcatcact aaactggata
     2581 aagaaggcgg tgaggcgttt ctggcaatgt ggtcaaaaaa agcgggagtt gatggtatca
     2641 ctgcggggat cgggattgtc gccggaaaag acagtgaagg taggcctgta agtcaggttg
     2701 caatttctgc gtcgcagttg tttgtctttg acccgaacaa cccggataac accgcctatc
     2761 cgtttgcggt atcaggtggc aaggttgtga tcccgaaagc gatgatttat gacgcggtga
     2821 ttgaaacact ggtgtcgcgg aaggttgtgg cggatgaggt aaaagccggg gtaagtatca
     2881 cttcgccagt tatccggagt gccgttattc agaacggaaa ctttcaggtt gattctcagg
     2941 gtaacctgaa tattggaggc cttttcagtg ttacgtcaca agggcaactg acaattcgtt
     3001 actctaatca gaatgtagga ctggtgatcc gcaatgataa aattgaggtt tatgatcaga
     3061 atggacgact ggctgttcgc ataggcagat tacgctgatc aggaggtgag tattggaata
     3121 cggttttgcc atttataaca gaaataacgt taatgttacg ggcgtgctga ctccggtatt
     3181 tttcctggac agatttacag cggagtctgg ctcaaagacg tacacgaata aacccgacgg
     3241 gaaatcattg caggctgtat gttgtttatt tccctggaat aacgtatttg cggatcggaa
     3301 agtaccgaag ataaccatta atggcaatac ggtgacgtgg tcgaatcttg agcagggtat
     3361 gggatcttat atttatacat tctggggata agtgtcatgt atggtttgag cattatgaag
     3421 ccggatggca gcgtatggat aagtccaggt tttacgccgc agtgtctgat caacaaaggc
     3481 accataccgg cgactgaaaa gtcttttttt aaaacatcaa tcccgtcagg caaaagttgt
     3541 tttttcttta tcagaacaga gaagaaggcc gatgtcatgt acacgcatga acagattgat
     3601 ggatatcatg cactaaggct tcatgtaatt gtcaggggaa cgaaccctgg tgttacgacg
     3661 gtttatgctt tcgcgaatat ggttactcca ccttctgagt atggtatcgc catgtataac
     3721 ccggacggtg agatgattta tcatggcgaa atgatgctgc ttgacgcgaa gttaatacct
     3781 gttgatatca aatttgaaaa ggaccttgga tatccatgcg caatcatgcc tgcactggtc
     3841 gggtattata actggaaaag aactccttat gatcgaccga tttataccac atccactggt
     3901 gctacaggaa ataaaatata ttcctgtgag cattattccg gcggtgcaac atgggatatt
     3961 cgaaagccgt atatagataa ggtcctggtt attaatacat cagtatatga ttagttgaag
     4021 cgagtcttta atattcattt aaaatgtcta aaaagatgta ttattaaaaa gtttagcgtg
     4081 ttatctgaat acaggatatc ttaaatgaag agtatagcaa cactggttgt gtgtgcaatc
     4141 tccgggattg cctgtgtaaa tttatctgca catgcagcag aaggaaacca tacaatttct
     4201 ctggggtatg cgcactttca gtttccggga ctgaaggatt ttgtaaagga tgcgactgct
     4261 cataacaggg agactttcag tcatttcgtc aacagaaact acttttcttc attgggcgaa
     4321 tatacagatg gtcgggtcag tggatatgaa ggcaaggata aaaatccaca gggcattaat
     4381 atcaggtatc gctacgagat aacggatgat tttggcgtta tcacctcttt tacatggacg
     4441 cgttctctca ctaactcaca gacatttatt gatgtgcagt cagccgatca taccaggaag
     4501 attaagaatc cggcagcttc tgccagaacg gatatcaggg cgaattactg gagtctgtta
     4561 gcggggcctt catggcgggt taatcagtac atgagcttat atgcgatggc agggatgggc
     4621 gttgctaaag ttagcgctga cctgaaaatt aaggacaata ttaacagtag tggcggattt
     4681 tctgaaagca acagcacgaa aaaaaactcc cttgcgtggg ctgcaggtgc acagtttaac
     4741 ctgaatgaga gtgttacact ggatgtggct tacgaaggtt ccggctctgg cgactggcgc
     4801 acgagtggaa ttactgctgg cattggcctg aaattctaac ctgtatccgg taaccgttta
     4861 ctacccgctg tgatggcggg ttttttattg cccgtacagg gcaaaaaccg taaattatgc
     4921 gtgggtgcct ttcggctgat ggctggaggg tgaacctgaa ggcctgatgt ggaaaggccc
     4981 cgagtcaact taacgttaac ccgaggccct aacactttgt accttgacaa gtagaaggtt
     5041 agcgcctctc cactcaggag gcaagcgcta tgtcgcaaaa atcgcttatc accgtcacaa
     5101 tttgtatgac ggttatcttc actatctgga tgttgcacgg ttcgctgtgt gagttccggc
     5161 tgaatttgtg gggagcggag tttgcggcgt tcttacagtg taagcagtag gaaaaccgcg
     5221 acggggacga aagtccccgt caactggttg ctgaggttca gccgatatgg cacccgtttc
     5281 aggtgagaga atgaacgata aaattctccg gtatatgcag cgtgtggtga gaaattcccg
     5341 caaccctgaa tttatgaatg aagttaaaga cgcctgcctt aaaaagcagg cgttttgttt
     5401 tgaggcacct gatggctttc tggtgctgcg ttctgtgctc agtgctgatg gtatccctta
     5461 tgttctggtg ttgctgggcg tgtgtacggg gagtaacagc gttgagcgtt atctgccgga
     5521 ggtgaagaca ttaacccgtc tggctggcgg acgttgggct gaatttcata cggcaaggcg
     5581 gggatttatc cggctgggaa aacgtctggg ctttgagcga atgccggatg atgaggatgg
     5641 cttcatggtg ttcaggatag cggtctgact gccacagttt tcatcatcgt gtttaaacca
     5701 acattgcaat tcacattctg accctgctcc ggcagggttt ttttattatc cagggggcca
     5761 ttatgggtgg aagtaaaggt ggtggtgata ccaaagtaaa accaacagca gcgcaaatag
     5821 cacaggaaga agtggcctgg aaagggtggc aggattacaa aaatatcctc cgcccggctg
     5881 aagataactt catggaaaag gtcgatgacc tgaacagtga gcagcagtac gacaatatcg
     5941 ctggcaccac aaatctgggg tatcagaaac agtttggcga agcacggaag gagctggcgg
     6001 gtaatcttgc tcagtccggt gttgacccgt ccagtggtcg ctttaacgcg gtaatgaatg
     6061 cgaaccagag tgatcaggta accgggcaga ttgacacaac cacacggggg caggtatcgc
     6121 aggcagataa gtatgttgcc gggctacagg atgttgctgc tctcggttct ggtcagaagg
     6181 cggatgcgtt acagagtttt aactctctgg cagacagcag tctggcaaaa gctaaatcgg
     6241 atgcacaggc ggcgtttacg aaacagcagg ggcgagcctc tcttgttggc gctggtctgg
     6301 gtgcggcagg tgcatatgcg atgcataagg ctggcggtag cggaggaagt ggcggtgcta
     6361 aaacacctgg cactggcgct aatgccattc agcatcaggc tcagaactgg agactgtgat
     6421 tatggagtac ggtaaatacg aaacacttgc gagatacggt tataccggag cagcccgccc
     6481 tcagggggac tggcagacat ccgcagcgct gacccgccag caatacgacg actggcgcac
     6541 cagatattta ccccgtgtag caaggctggc tgaccttggg gagaacaaca gtctgatgaa
     6601 tccacagctt gcacgggtgg gaggccttgc cacttccagt ctccgtacag cgcagatggc
     6661 gcaggataac cagatggcga gatacggggt aagccgcccg gataatcccg acagtaatac
     6721 gctggggtta cgtaatgccc tggcaatagc aggtgcgaaa aatggtatcc gtgaagccga
     6781 acaggatcgc cagatgaata ttctgacggg ggcttctgca ccggcaagac agaaactgag
     6841 tgttggcgga caactggtgg cagcgtaagg gggcaatatg ggatacggtt tactggatat
     6901 tgcaaatcag tcgcggcgtg aggcattaca gggaataagt gacgcagaca gacgacgtac
     6961 agagattgag gcatcaaata aacaactggc tgctcaacag aaagctcaga aaaaacagaa
     7021 tatcggtacg ggcattggta cgggggccac tatcggggcg actgttggtt cagcagtacc
     7081 ggtagtgggg acagccgtcg gcgctgttgc tggtgcagta attggcggca ttgcaggcgc
     7141 tttgttttaa ggagtggtga atgagcggat ttgcacaggg gttacttgcc ggattcagca
     7201 ctgttgacca ggcaatgacc cgtcgtaagg agcttggttt gcgagaagca cagcttgctc
     7261 agcaacagaa aaataacgag cgcgattttg aatttgcgca gtctcagttt gaacataata
     7321 aagacgttga tcagcggaac tttgattaca gagccaaagt tgatgaccgc aattacacac
     7381 tgcaggaaag ggagtttaac gccaaccaga attaccggaa tgcgtcgctg gggatggaac
     7441 agcaacgact ccagttgcag aaatacaacc agcgacggct tgagtataac gatatgctgg
     7501 cgcgcgatca gcctgtgatg gcggcgctgg gaaaagcgat tgatgcgggt gaccatgatg
     7561 cggcttctca tctgttcggt aaattatcgg acgccaatcc gcttaagatg atgtcaacgg
     7621 taggctatgc tgcgaaagcg ggtcaggccg tgaacaacct gcagaaaatc tttgatgata
     7681 agccggacag ggcgattgct tcgctgaata ccccggaaaa tctcgatgtg ctttccggcg
     7741 tgtttgcccc ggaactacaa cagcgtattg gcatgcctga ttcaaccggg gaaaaaacga
     7801 taaaagaggc caggattggc agtatcgtac cggcgcagca ggaagggtac gtacttattg
     7861 gccttgatct cacatacagc gatggctcca ccgcgcataa acctgtaaca gaatacggca
     7921 gtgcgcaccc tgatgatcaa accgtgctgg cgatacccgt tgataaggct atcgctcagg
     7981 tcagggatcg cagcaaattt gcagagatat cgaaaaatta tggttatttt atgccgaagc
     8041 agcagggact ttctctgaaa gagcttcaga agggggccag caacgtagcg gcggacgcga
     8101 tcaagaatgg cggtaatgct caggctgcgg tggatgaata ttatgctgcg actggttcac
     8161 aaccgcatca acagaaaatt cagcaacaaa aacttcagca acaggttatc aactgggcgg
     8221 gagatgatcc tgataagctg tcatttgcca gaaatgtagc ggcccgtcag cctgaaatgc
     8281 tggaacctca gaatcagaaa ttgctggaga acgggtatgc gaattttctc cgtattaaaa
     8341 aggccagggg ggaacaggcc agagatgaaa gtgcttcatc tgcatctcag tttatccgtg
     8401 gactgaaaca gaattacgcc cagtaattca cgatattcca ttaacaccat ttcctgatgc
     8461 ccggccattg tgccgggttt ttttatggag tctgtatggc ctattcagag gaacagcgtc
     8521 ctgaggcgca actcggtaac cagaatcgta acagcctgaa cattcagcaa cccggcgaaa
     8581 ctgacagcta tgaagcattt ttctctgatc cgaatcgctg gaaggataac agtacgtcgt
     8641 tcagcctggg cgatgtattg ccaacaatgg gtaaaggttt cgcccagtcc gtccggggaa
     8701 caggggaaat ggcccgtgga ctcggtgatg cgatgattca aagcccggta aaaacagggg
     8761 cgcgtatttt aaatgagttc agccgtatgg ggctgccggg tgtcgcaact gtgcaggata
     8821 tttttgccgg tggcagcagg ggggctgatg aggtcatcga taccctgcct gatggcaaaa
     8881 acgcggttac tgatactgtc ggtaaaggtc tgaaggcaac cggtaaggct gtcagtgatg
     8941 gtgctgaagc cactgatgaa tggctgaccg gtaagatgtc gccgggtgct gttcgtgcgc
     9001 tgaatacgcc gatgaccgaa ggctatgatg attctgcggt ctgggtggcg aagggtgtaa
     9061 acctgattgg tgcgcttgta cctgatatgg ttgctggcgg tgtggctaaa aaggtgggtg
     9121 atgtcacact gcgaaaaatg ctgaccgccg ggctggagaa aaaatacatc gcggcaggga
     9181 tgcagccgga aagagccacg gcactggcag cagaagctgt cgataaaaaa atgccggatt
     9241 tattccaggc gggcctgatc acccattcca cagccagtgc acaggggcag agtgcaatgg
     9301 cggcagcaga tgctgttctt aatgcggatt actctgagct ggcgcagtca ccgaaatttc
     9361 agcagacgtt tttgtccatt gacgccgacc cgcagcacgc acagcttact gatcgccaga
     9421 aaatggatct ggcaaaagag cgtgttgccg atgaggtgcg cgcgcagctg gcaaccgatc
     9481 ctgaattgct ggctgtgaat gccatggcgg caaaactggg tgacgcacaa ctgtttaatc
     9541 tggtgacacg aggcacagcg aagaccgtta aaagcggcat tgtcagaaat gccacggaac
     9601 agggggcgat taatgcggcg cagggcggct attcacgcta tcaggaaaac acggcattgc
     9661 gtgagaccgc cggaatggat gtgtcaccgt gggagggcgt ggctgacgca acgatcgaag
     9721 gtgcagcctt tggtgctgcg atgggggctc catccggtgc ggttgccgga tatcgtggca
     9781 gacgtcaggc cgcagaagaa accgccatgc gtgatgctga aaccgtgcag caggacgacg
     9841 cagccccgca accagaatct gttgatccgg tggcgcagca gcgtgaatcc atgcagggca
     9901 tgaatcgcga gcagcttctg gagcagtatg ctgatgcgga tatggcaaca gagggtgacg
     9961 tatccgcagc tcatcgccgg gaagctgcca gccagttgtt gaatgaactg gacgaacagg
    10021 cgaagcgaca ggctgtgatg aatgagctga aggcgaagcc gcgttctgaa ctgcttgagg
    10081 aataccgcag actcagccag aaagaggagc gcaccgagac tgaagaacaa cagtttcagg
    10141 caatacgaga agtcattcgc ccacaacagg aagtgacgcc ggaagcacag tcacagcctg
    10201 aaaatgcgga ggatggtaac gggagcattt acccgacggt gcggttccgg gacccgaatg
    10261 aagtccgcat tgaaattaac gggaatggtg cgtccagacc agcggaacgc attgaaaagg
    10321 tgcgcccgga caaccgttat ttcacggatg agaaaagcgc catggggagt gatgttttcc
    10381 gtaatgccgc cgccaccggc ctgaaaccgt ccgtagtgaa gaaaggcgag aatcaatatg
    10441 ccgttgaaat ggataatcct gcgttctctg aagatgtggc aacggaaacc attaacaccc
    10501 tggctgacgg agagcgtatt gctgatgctg acccgatgga gcagcccgcg ttcatgcgtg
    10561 acccgcgatt ccgtggtttc acgggggatg atacggaggt acaggcccgc cttgcccgtg
    10621 gcaacgcgcc gacggcagag gagcttgtac gttcacagat ggctgaaggt gatgccggtc
    10681 cgacagcaca ggagttaact gagcgtccac gcctgcccgc tcccggcgat attcatcccg
    10741 gacagggata tccgttaccg ggagaggtgg cgcgtacgcc ggatgagaat caggccggac
    10801 gtggtggtcg ttttaccaca accggtgagg ttaagggcca gagtttccag aaaggacaag
    10861 ctccggcacc ggaaaacgcc gctggtcgcc agggggaaac actcgagggt gaaatggttc
    10921 gtcgtggtct gccgtcaccg gatgcgcaga acgcgacagc accggtacgt gaagggctac
    10981 cggctcctga cattgcgcgt aatgttcgta tgcctcagcc tgaatcactt ccccgcactg
    11041 tacgggactc actgcctgag cttgcacagc aggcagaagt acgccgacag gccggaggaa
    11101 atcgtgacat cccgcagcct gagacaatcg cacctgaatc tgaaacaact gtctctactg
    11161 acagggaagc taccgtgcgc ggaggtgaag tcaggggcaa aaaaattgaa gactttggcg
    11221 aggaaattaa aggggcagcc aaacaccgtt atgcacagct tgctgaaaca ctgggtaaaa
    11281 cgctggaaga cagggattat gccacgcagc cgctgagcaa actgttcccg aaaccggact
    11341 acgcaaaact ggcgaacgaa ggtgccgatg ctgataccct ggcaatgata gcgctgtatc
    11401 gtagcgatat tccggcgaag acgaaacaca atacggcagg ctggggggag agcataaaaa
    11461 aagtacgaca cagtgtatcg gaaatgctga acggaacggt cagcgcgaaa cgcctcgcag
    11521 aatggatgga aggcagaatg ccctcccgtt acgcggacac ctggcaactg ttacgcactc
    11581 tgccaccctc acagatggac agagcttctg cttatcgggt ggtatcgggt gtgtatcagg
    11641 cggcaggagg gaagcgttac gatccgccac agaaacttta ttcactgcgc aataaggaca
    11701 ataaggggag taacctcttt ttctcggaaa gcagggatga attactggca aaggcgaaag
    11761 tctggtttgc agagcaggag gaaaaatcac aggcgaaagg tgatgaaaaa acagcaccgt
    11821 caccggatga caaaatccgc tttgacgttt accggaatac ccgcagtggc gatattttta
    11881 tcgcttacgg taaaaacaaa atgcgggtga gaggtggctt taagtcagcc agtgatgcgc
    11941 gtaagtacat tgattcacat cgtgatgagc ttgttcgtca tgtgaaggag atgcgggaga
    12001 tttcgcgtga ggagcagcgc aacgccacca accgcgaccg taccggacca gaacgccgca
    12061 aggggaatgt ttcaccggag cagttcagtg atgcgtttgg tttccgtggt gtgcagtttg
    12121 gtaattatgt ggaaggtccg cgtcgtcagg ctgatttgaa ccgggcttat gactcgctgc
    12181 atgaccttgc ggaagtactg aatgtaccga caaaagcgct ttccctgaac ggtcgtcttg
    12241 gcctggcatt tggtgcccgt ggtaagggta aggcggcagc acactatgag ccgggtgagg
    12301 tggcaatcaa cctgacaaaa ggtaacggac cgggtgcgct ggcgcacgaa tggttccatt
    12361 ctctggataa ttattttggt cgttatgacg tttccaatga cgggaaaatt acgtcaggtg
    12421 gtgactttat gacggaagca cagcgtgtca ggcgcatatt taaagacggc aggtatgttg
    12481 atgctgaata tccggtacgt caggaggttt acgacgcttt taaaggtgtg attcaggcca
    12541 ttaaaaacag tgacatgccg cgtcgttcag cgcttctcga tgaggtgcgc tcaaaaccgt
    12601 actggtcaac ggatgttgaa atggcggcac gtgcctttga gcgttatgtt caggataagg
    12661 cgcgtatggc tggcgtggag aatgattatc tggtcaatat ccgtaaggcg cctgagcaca
    12721 acacagataa cacctgggct tatccgacga atgcggaact ggatggcggt attcgtgatg
    12781 cattcgatca cctgttcagt acgctgaaaa cccgtgagac ggacaaggga gttgcgtttt
    12841 attcccgtaa gggcgtgacc cgcacacctg aaggcaatct catttcggat gttaaccgca
    12901 gtgcggaagc caaaggcagc ccggtcccgc aggttgaagc ggttgtccgt ggcgtgatga
    12961 gcggcattaa ggacagtgat ctgaaggtcc gtgtggtgaa gtcacagaaa gaggctgaag
    13021 cgctggcggg cgaattgttc gatggttacg gcagggtgca cgcattctat cgtccggata
    13081 aacgagaaat tgtcctggtg gcggataaca tccctgacgg gcggaccgta cgcgagaagt
    13141 tgcgtcacga gattatccac catgccatgg agcatgttgt cacgccagcg gaatatcaga
    13201 cgattatcaa aaccgtgctg aaaacccgcg acagtgataa cgccaccatc cgtgaagcct
    13261 ggcgtaaggt tgatgcgtcc tatggtaagg aatcaccgga agtgcaggcg ggtgaatttc
    13321 tggcgcatat ggcagagaaa cagccgaata aatttgtggc ggcatgggag cgtgttgttg
    13381 ccctggtcaa aggggtactg cgtcgtacgg ggttactgaa accgacggaa ctgaacgata
    13441 tcagacttgt tcgcgagacc atccgtacgt taggccagcg tgtgcgggaa ggttacacac
    13501 cgcgtgagga tggagtgggt gcatcgtctc agtactcccg tagtggtaaa cctgatccgt
    13561 ttaaggctga cgcctatgat gcaaagcagt ttgcttcaga agtgcgtcgc attgccggat
    13621 tacaatcggt ttccagaagg gaaatgctca gagttgggga tacgcctcgt gttttgcgcg
    13681 cattgggggc accagatctt gaactggtta tgcctgcatc tgttgtgcat aaggctacga
    13741 gaccagagat cagagaacac tttgtgtcag tggaaacact ggctgatttg cctcaacttc
    13801 tgacagatcc ggtagtagtt atgcgttcga aaactgaatc ggatgcgttg gtatctttaa
    13861 ttcaggccac cgatacaaaa gggaatcctg tcattgttgc aattcatacc catgtgaaag
    13921 gcgatggatt tgcagagata aacaaaattg ccagtgccta tggaaaggac aaaaggagtg
    13981 ctttgcagga tcagcttgat acatctttgc tgtatcttaa taaagaaaaa gccgctgaat
    14041 ggcttcatgc agtcgggctc caattgcccg aggcgaacac catcaacggc tcctctggat
    14101 ttaaaatact acatcctgag gatattcgca aggggccgta ttactcccgt accagcagtc
    14161 tgacgccgga ggatacaatt gcatcgcgtt ttgtgcgcca gatgcaggat aaattccagg
    14221 tgctgaaagc tgttcaggag aatatccgta aaactggcgg aaaaatagac gacagtaaca
    14281 acgcttatat ggcggaagaa ctcttccacg ggaaggcgga aaacgacctg aacgtgatga
    14341 aggagcgcta tgttcagccg ctggctaaat tactggcgga ctataagatt gcgcaggccg
    14401 atctggatga gtacctctac gcccgtcacg cgccggaacg taacgcgcat atcgcgaaaa
    14461 tcaacccgaa aatgccggac ggcggttcgg ggatgaccaa cgcggaggcg gcggaaatca
    14521 tgcagcgtgt gcgtaacagc ggcaaacagg cacagtatga ccgtctggca gggattgttg
    14581 acgatatgct ggcccgtcgc cgtgagctta tccgtgaggc cggactggaa gagagcggtg
    14641 tggtggatgc ctggcagaac gcctaccgtt actacgttcc cctgaaaggc caggatgttg
    14701 acggtgtggt gtcactgccc cgtacaggta agggcttcac catcggcgga cgtgaaagca
    14761 ggcaggccat ggggcgtgca tcccgtgcac agtctccgtc cactcaggcg atacaggacc
    14821 tgagcgaatc gctgatccgc catcgcaaaa acgaagtggg taacgccttc ctgaaactgg
    14881 tgcaggataa tcccgacaag gattactggc aggtattcac tgatgacaga ccggatacca
    14941 tgcgtgtgat tgcagagcgc aaggaccagg aaactggtga aaccattcgc gaagttgtcg
    15001 agcgtccggt ggcgatggca atgatggcag accggtactt caccaccaaa aagaacggca
    15061 aaacgtacta catcaaactc catgacccgc gcctgatgcg tgcgatgaag aatatgggac
    15121 cggaaaccag taacgccgta atccgtacgc tggggaaagt taaccgcttc ctggcaacgg
    15181 tgaacacgtc gtataacccg gaattcctgg tcagtaactt catccgtgac gtgcagacgg
    15241 cggtgatgaa cctgaaggca gagcagggaa ggagcgacgg taaactgaaa gggctggata
    15301 acttatccgc cctggctgta gtcaaagaca gccgttctgc aatgtcagcc gtgtacgcca
    15361 gtctgcgagg caaaactctc acgggcaaag gtacacagtg gcagaaggtg tggaaagagt
    15421 ttgttgagga cggaggcaaa accggctggt ttaacatggg tgaccttgaa ggccagcaga
    15481 aggaaatgga tcgccttgtc tcactggcga aggggggatg gaaaggccag agtatcggtg
    15541 catggaattc gttcctgaac ctggtcgagg atgccaacgg cgcggttgaa aacgcactgc
    15601 gtctgtcagc ttataaacac gcccgtgatg ccggtttgtc acgccagcag gcggcgtctc
    15661 ttgccaaaaa catgacggtg aactttaacc gtcgtggtga gcagggggcg ctgatgaact
    15721 cgctgtatat gtttgccaac gccagcattc aggggaccgc aaacctggtg agaacgctcg
    15781 gacaccttaa tggtgaggga cctttactgg agcgccttcg ctggaagaat ctgaatgttc
    15841 cgcagaaaat cgcgcttgcc gctgtgggag caggttatct gcttggctcg cttaaccgca
    15901 gtgtggcggg tgaggatgat gacggggtta actggtatga caaggtgccg tctcatgtga
    15961 aagagcgtaa cctcgtcatt atgaaatcgg tgttcggggg caaggccgga gagtactgga
    16021 gtattcctct gccttacggg tacaacgttt tcttcctgct cgggcatacc gctgaaggtg
    16081 tggcggcggg tgacctgacg gcgtcccgtg ctgccggtaa tgttgttggt ggtgtgcttg
    16141 gtgcattcag cccgattggc agtgagacgt cggaaacact gtccggggca ttgctgaaaa
    16201 atgcagcgcc gaccattctg cgtccgtttg cgaaccttgc catgaatgaa aacttcatgg
    16261 gggcgcagat ttaccaggag aacatgccgt ttggtacacc aaaacctgac agccagctgg
    16321 gaagacgttc aacgccagaa gcgtacaagg cgtttgcatc ctggctgaat gcgttctctg
    16381 gtggcagcca gtaccgtccc ggcgcggtgg atatcacacc ggaatcgctg aaatactggg
    16441 tggactatat ctccggaggg acagggcgct tcatttctaa aaccacggat gcggcggtga
    16501 aatcgctgaa tggtattgat ataccggagc agcaggtgcc tttcctgggg aaaatttcag
    16561 gtgaggtgat gccgtatgct gaccagcaga agatgtacga ccggatgaca gaggttgcgc
    16621 agtatcacgc agagctgaag agtctgaccg gtgcagaaag aacggcgttc attgacgaga
    16681 acaacggaaa attgtcgatg aacgggctta tgcaggatac ccggaagaga ctgaaggatt
    16741 tgcgtaaaca gtgtgatgcc atttacgccg acagtactct cagtctggcg caacagtcgg
    16801 cgatggtgaa atcggtagag cgggatatga aaattgccgt ggatcggttt aaccgcgagt
    16861 acaacaaaaa agtgggagtg gagtaaagaa catgccccgt acggaagtgc ggggctgata
    16921 taaagtcgcg cagggaaata ttgtagataa actgaacagt taaattagga taatgttgtg
    16981 atattgatca gaaattacag tgtaagcaga tgtgaaacag catgcttgat gagagtaagt
    17041 tttttctgat gcctgttccg gtggtgtagt cgtggttctg agtgactaca ccaagaataa
    17101 tgtaagacaa atcctaagat tattaaatta atattttggg tttattttcg gaatatcctg
    17161 aataaagccg taattaatca tatttcctta tgggtatgaa tgatgtgttg cggctgattt
    17221 gtatacagat aatttaacag gatatccata atgagtaaaa aattcacaaa gacacttctg
    17281 tcatcttctc tggtagggct tctggttgtg agtgctgagg gtttcgcaca ggtatcagta
    17341 tatccgaaag gctcgcagta tagcgtttta gtggatgacg agaataaacc gaatgaattc
    17401 ggtagcaaat tcaaagttta ttacagtaag tcatctcacc ggggagttgt gcgatatgtg
    17461 caaaatggac aagataatgt tctatttcac ttcggtaatg acggcaatat ttttgtgctg
    17521 agcaaggacg ggcaaatagg atataccgtt catgagcctg tgttgaagga ctttgcccgt
    17581 atggctgaag gtcttaaaag tgatggaagt aaaaaccata agctggatga ggctgaagta
    17641 cagcgtattt acgataaaat ttataacatg tctaacactg ttgtaaatcc tgcattgaaa
    17701 aatcaagttg gtgagctttc tggtcgcgtt gacaagattg atctggctgc aggagctatt
    17761 gcaactgatg ttgcatctaa aactacggtt ggtgtgaaca gtgatggaac tctgaccagc
    17821 gctgatggcg ctacaaaaac gattgctgtt aatgacggtc tcgttgctct gtccggcaga
    17881 acagaccgta ttgatgctgc agttggtagc gttgaccgcc gggttactaa aaatacacag
    17941 gctattcagt ccaatactcg ccagttgcag gagcataatg cacgcctgaa cagccagcag
    18001 cgtcaaattc gtgagaatca cgaagagatg aagcgtgcag cagcacagag tgcagcgctg
    18061 gcggggcttt tccagccgta cagtgtgggg aaattcaacg ccactgctgc cctggggggg
    18121 tacagtgata aacaggctgt tgccgtgggt gttggttacc gtttcaatga gcagacagca
    18181 gcgaaagcag gtgtggcagc gagtgatggc gatgtgtcct acaacgtagg tgtgaacttt
    18241 gagttttaat tgctgacagt gacatgaagc atgacggcag gcgtggagtt ttgcctgccg
    18301 ttttttctca tgtgagcatg aaacaggtga cagcgatgaa agtgattctg gcgacccgca
    18361 accgttatct ggagtatggc ctgcagcaga tgcttgaggg gtacaggata atactggcga
    18421 gggagttttt tacaccagag aacaggaaga gtgtaccggc acatgatgag tcatgggtga
    18481 taatctgtga tgccctcctt ggcaggctga tgtgctgcat gtttcaggga cgccgttatc
    18541 ttcagattga tgcggaagat gttacggggc gcctggagac gtacaggaaa atccggaatg
    18601 gtgagtgggt acataatacg tatgcgcgac ctctgacgat gtccgagatg gtggtgatgt
    18661 ttggatacgt gtaccgggag tcaaagccct gccatcttgc ccgtgaaatg gggataaaca
    18721 cgaagacggt gaacaccttt ctgtatatgg gacttggtaa aaatggtctt aagtacagga
    18781 gtgtgaagca cctggttgga cgtgcgtagc cggaatgcat attcgtgagt cgggtaatga
    18841 gagtggaacg gtggcagtga gaatggaaca gaaaaaaatc agatgcggaa agcggaaata
    18901 cacagcgggt gcgatgtgct tcatgctgtt tgtatggctg gggggtgtgg tgacattctg
    18961 tgtgctggcc gctctgataa tgaggtgaaa acggaagaga gtttacttcc ttaattcgaa
    19021 ctttctgata actgagaact tgttaaatgc ctgtgggttt atgtatttaa atcaattggt
    19081 taatggtgat tttatcttta tcagtcaaag ttcgttttag tatgatgatt gtgcccgctg
    19141 tttattgttg tccgggagaa aatacaggga atccagggga tgaacaaaat atactcactc
    19201 agagttaatg acagaaaaga actggttgct gtaccggaaa cggtgggggg caggaaaaag
    19261 tcgtccggaa ttcccggtgc ggggatgctt tcccgtcttc ttctggcctc aggagccgtt
    19321 gccggcgttc tgttttcttc tcctgcgctg gcatccgttg tcgggaatac tctgccatgg
    19381 cagacctaca gggattttgc tgaaaacaaa ggggcgtttc atgccggggc cacaaatatt
    19441 ccgctttatg gtaagaatgg ggctgtcagt gggcggctgg acaaggctcc gatgatggat
    19501 ttcagtgttg tggaccagat actgggggtg gcaacactga tttctccgca gtatgtggcg
    19561 ggggttaagc ataacggcag ctacaatacg gtgcgttttg gttatgcgga cgatacgaca
    19621 tatcgccttg ttgaccgtaa tgagcactgg cgtgattttc atactccccg gctgaacaaa
    19681 ctggtgacag aagttgcccc ggtgagcgtc acggatgccg gaaccggcaa gggggtatac
    19741 cagaaccgca gtcgttatcc ggtgttttat cgtatgggga gtggaactca gtacaccgga
    19801 gctgcttccg gtgcgctgac cagaatagcg gatgcttacg tctggaggac tggcgggacg
    19861 gtgggaagtc cggtcatttc tgactggagt ctggtgtcaa acccgggaaa tctttaccag
    19921 ggtgttaatg gtccgctggc gtcttacggg acatcaggag acagtggctc accgcttttt
    19981 gcctgggatg cggtgaagaa gcagtgggta tttgttgctg tacttcatgg ttatgccggt
    20041 gagaacggga agacgaactg gtttacggtg attcctgcag gggatgtgaa taacacgata
    20101 aagcaggaca attcaggcac agttgtgccg gcagttgccg gtggtgacat tgtgtggaag
    20161 tacagtcagg gaagcggaga gggaaccctt tctcagggcg ggcaggtatg gaagatgaac
    20221 ggcttcaggg gagggagtct gaatgacggg aaagatatca cctttggggg taagggcacg
    20281 gttgttctga aggatgatgt tgtccagggg gcgggttcgc tcacgtttaa tggtgattat
    20341 acg
//
LOCUS       DAZFKF010000090        20330 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFKF010000090 DAZFKF010000000
VERSION     DAZFKF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.090"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            277..777
                     /gene="tssB"
                     /locus_tag="ACHKA1_003830"
     CDS             277..777
                     /gene="tssB"
                     /locus_tag="ACHKA1_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO5681421.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            812..1036
                     /locus_tag="ACHKA1_003831"
     CDS             812..1036
                     /locus_tag="ACHKA1_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681422.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1087..2562
                     /gene="tssC"
                     /locus_tag="ACHKA1_003832"
     CDS             1087..2562
                     /gene="tssC"
                     /locus_tag="ACHKA1_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO5681423.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2569..2982
                     /gene="tssE"
                     /locus_tag="ACHKA1_003833"
     CDS             2569..2982
                     /gene="tssE"
                     /locus_tag="ACHKA1_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO5681424.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2986..4836
                     /gene="tssF"
                     /locus_tag="ACHKA1_003834"
     CDS             2986..4836
                     /gene="tssF"
                     /locus_tag="ACHKA1_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO5681425.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTMGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4800..5882
                     /gene="tssG"
                     /locus_tag="ACHKA1_003835"
     CDS             4800..5882
                     /gene="tssG"
                     /locus_tag="ACHKA1_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO5681426.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALIRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5907..7187
                     /gene="tagH"
                     /locus_tag="ACHKA1_003836"
     CDS             5907..7187
                     /gene="tagH"
                     /locus_tag="ACHKA1_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO5681427.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDHNDDISAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7184..7708
                     /gene="tssJ"
                     /locus_tag="ACHKA1_003837"
     CDS             7184..7708
                     /gene="tssJ"
                     /locus_tag="ACHKA1_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO5681428.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYMTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7711..9042
                     /gene="tssK"
                     /locus_tag="ACHKA1_003838"
     CDS             7711..9042
                     /gene="tssK"
                     /locus_tag="ACHKA1_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO5681429.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9047..9808
                     /gene="icmH"
                     /locus_tag="ACHKA1_003839"
     CDS             9047..9808
                     /gene="icmH"
                     /locus_tag="ACHKA1_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO5681430.1"
                     /translation="MDEGSLSLPPFTGYDEKGQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILYQLNKLLR"
     gene            9817..12579
                     /gene="tssH"
                     /locus_tag="ACHKA1_003840"
                     /gene_synonym="clpV"
     CDS             9817..12579
                     /gene="tssH"
                     /locus_tag="ACHKA1_003840"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO5681431.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIDMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYILPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDASDTLLARYAKNMTEDARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSVVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEVAWHQQQTLVREIIALRQQLLGMAEDT
                     DAVVDTSSESESESESESEQDNTAAIPADDAGSVQPEETAETVSPVQRLAQLTAELDA
                     LHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPVWLGDTIKGQDLAI
                     ASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMS
                     EFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQA
                     FDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPEIMQEALYPVLADFFKPAL
                     LARMEVVPYLPLSKETLATIIAGKLTRLDNVLRSRFGAEVIIEPEVTDEIMSRVTRAE
                     NGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAADGAFTADVEDAQDDQSVT
                     EDEPVL"
     gene            12576..13319
                     /gene="vasI"
                     /locus_tag="ACHKA1_003841"
     CDS             12576..13319
                     /gene="vasI"
                     /locus_tag="ACHKA1_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO5681432.1"
                     /translation="MSRMRVFRQTGWFIAGLMAGLPASAAPAETSSMAGVAVAVATAT
                     PLDAAATLQAMQSCRRESAALERLDCYDRLLEPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13324..14739
                     /gene="tssA"
                     /locus_tag="ACHKA1_003842"
     CDS             13324..14739
                     /gene="tssA"
                     /locus_tag="ACHKA1_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO5681433.1"
                     /translation="MAMDLRDPNVWISHLLENLPDEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWSVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELALATDDLFALYQRAFNRAAPAPAPTPAASDSSPQTTATSESGVTQPSTPTPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMTLWQQVEKSVLLAPYWLDGHCISAQTAHRLGYTAVADAIR
                     DEVVSFLGRLPALADLLFNDHTPFVSEKTKQWLATQPGNQAAPVIQVSNEDIQAARLC
                     FDEQGLEAALRYLDTLPAGEPRDQFHRQYFGAQLMEEAGLVQLAQQQYRMLFRMGLQM
                     MVADWEPSLLEQLEQKFTAEQ"
     gene            14848..18282
                     /gene="tssM"
                     /locus_tag="ACHKA1_003843"
     CDS             14848..18282
                     /gene="tssM"
                     /locus_tag="ACHKA1_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO5681434.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVQVMKRLQQLEKMQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGTEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSTTGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPSVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18293..19703
                     /locus_tag="ACHKA1_003844"
                     /pseudo
     CDS             18293..19703
                     /locus_tag="ACHKA1_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19669..20148
                     /locus_tag="ACHKA1_003845"
     CDS             19669..20148
                     /locus_tag="ACHKA1_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO5681435.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQIMIYEF
                     MHGLNRDQNVNHHHLTIKKPIDKASPLLGKAICDNELLTCDFSFYRTNKFGINELYYK
                     IKLTGAKISDIHVSISHIVVDNSVQPEESVSFSYESIIWEHCSAGTSAYSLWEDRLF"
     gene            20169..>20330
                     /locus_tag="ACHKA1_003846"
     CDS             20169..>20330
                     /locus_tag="ACHKA1_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_302948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681436.1"
                     /translation="MANNILHSSTSDSESKNTSTDSTLFNEKFPFQLRQDFASRITED
                     ESDFFKWKKI"
BASE COUNT         4828 a         5532 c         5568 g         4402 t
ORIGIN      
        1 caataagata cataaaaatt ctatatttaa attattgcca taatacacct gacgcgaatt
       61 gcacaggttc atacaatcta tttaacgctt gcagaatacc cctctttacg atcgataaaa
      121 agtccttttc ttatttattt tgaagcgtaa aatgcggggt gatgaaggag tggtgatttt
      181 attttcaccg ccatcaaggc ggcatttccg atattttttg tggtacgaat atgccgtgtt
      241 catggcctgt acgagataac atgacggatc acttttatga gcaaaaaatt tgaaggcagc
      301 gtcgcgcctc gcgaacgtat taatatatcg tatgtcccaa aaacagatgg ccagacggct
      361 gaagtggaac ttccgcttaa catgctcgtt gtcggcgata ccggaaatac gcaggaaaca
      421 tcctcactgg atgaacgtca ggcagtttcc gttaacaagc acaatttcgg tgcagtgatg
      481 gctgaagcag ccattggcct caattttacc gtccccgcca cgctgaaggg aagcacaacg
      541 gacgatgagc tgaatgtggc gctgaatatc aagtcactgg atgatttttc accagatagc
      601 gtggcccggc aggttccgga agtgaataaa ttgctggaac tgcgtgaagc actgactgca
      661 ctgaaggggc cgatgggtaa ccttccggca ttccgtaccc agctccaggc attgctggaa
      721 aatgaagagt cccgcgagca gttgctcaaa gaaatcggcc aggtgagcaa taaataacca
      781 ccactatttc attaaacagg gttatccagg catgacggca cacaaaaata tctccgggac
      841 ataccacctc tctgttgcag acattctgca ggtggtttac caggtatgtt tcagcccgtc
      901 cgttgaaatc aaccaggatg gtgtggctgc gctgattaca acactggatc gccgtatcag
      961 tgatctattg gatgagatta tccatttttg tgagtttcag cagagcgcgt cgcactggca
     1021 gcgcgtactt cactgacggc gaaaagccgg tttcagaaac acagctaaaa cagaaggatt
     1081 ttcagtatgt ctttacagga agaggaactc gtttccagtc atgccgggca gcctgagcag
     1141 gcatcgtcgc tgcttgatca aatcatggca cagacccgta ttcagcccgg ttcggaaggt
     1201 tatgacgttg cccgccaggg tgtgacagcc tttattgcca gcatcctgca atccactgca
     1261 tctgccgaac cggtcaataa actggccgtg gacagcatga ttgcagatat tgatgaacgc
     1321 atcagccgcc agatggatgt catcatccat gcgcctgcct ttcagcaggt cgagtcgttc
     1381 tggcgctcac tgaaaaccat ggtggatcgc gttgatttcc gtgaaaacat caaggtcaac
     1441 gtgcttcatg tcaccaagca ggaacttctg gaagactttg agtttgcgcc agaaattatt
     1501 cagtccggat tttataagca cgtttattcg tccggtttcg gacagtttgg cggtgaaccc
     1561 atcgctgccg tgctgggcgc ttatgagttc aaaaataccg cgcctgacat gaaactgctg
     1621 caatacgtca gtgccgtggg cgcaatggcg cacgcaccgt tcctgtcctc cgtttccccg
     1681 gagtttatgg ggctgaactc gtggaccgaa ctgcctaata tcaaagatct ttatgccatc
     1741 tttgaaggtc cggcctacac caaatggcgc gccctgcgtg actcggaaga ttcgcgctat
     1801 ttagggctta cggcaccccg cttcctgctt cgccagccct attcaccaac agataaccct
     1861 gttaagaact ttaactatta cgaagatgtc agccagaacc acgaagatta tctgtggggt
     1921 aatacggcct ggatgctggc atgcaatatc gcagacagtt ttgccaaata ccgctggtgt
     1981 ccaaacatta tcggtccgca aagcggcggc gcagtgaaag atctgccggt gcatctgttc
     2041 gaaacgatgg ggcagattca ggccaaaatc ccaaccgagg tgctggtcac cgatcgccgc
     2101 gaatttgaac tggctgaaga gggttttatc acccttacca tgcgtaaaga ctctgataat
     2161 gcagcctttt tctctgcaaa ctcggtacaa aaaccgaagc actttccggg aaaagatgca
     2221 gaaaccaact ataagctggg tacgcagctt ccttatctct tcatcatcaa tcggttagcg
     2281 cactacatca aggtgttgca gcgtgaacaa ctggggtcat ggaaagaaag gagcgactta
     2341 gaaagagagc ttaatacctg gatccgtcag tatgttgccg accaggaaaa cccgcctgca
     2401 gacgtgcgca gccgcaaacc tctgcgcgct gccaaagttg aggttatgga tgtagaaggc
     2461 gaacccggct ggtaccaggt tgcgctaagc gtgagacctc attttaaatt tatgggggca
     2521 aattttgagc tttccctggt tggccggtta gacagggagt aacacaccat gataccggat
     2581 cgggaaagaa ccacaggttc tctttttgag cgtatggaag cctcttcggc cagaaaccgg
     2641 cagggaggca gtattcattc gctcaggcaa tccattcgcc agaacctacg aaacattctt
     2701 aatacccggt ccggtagttg ccggggtgcg cctgagctgg gcattgatga accggaaggg
     2761 gctgaaaact tccgcgaatc catgagtcgg gccatcgagc aatgtataga acgctatgaa
     2821 ccccggatct cacacgctga agtgcaggca gtggtttcat ccgcctccag cccgctggat
     2881 atgacgtttc atattacagc ctgggtgaca tttaacgaaa cgcatgaggt gcttgaattt
     2941 gatatggcac cgaatggcag ccagcattac cgggtggact gacaaatgga atttgaagaa
     3001 cgctattttc gggaagaact cgattacctg cgccagctta gcaagctgct ggcaacggaa
     3061 aaaccccatc tggcccgctt cctggccgaa aaagatgcgg atccggatat tgaacgcctg
     3121 ctggaagggg tggcttttct taccggcaat ctccgccaga aaattgagga tgaattccca
     3181 gaactgacgc acgggcttat taagatgcta tggcctaatt acctgcgtcc ggttccggca
     3241 atgaccctta ttgaatatac gccggatatg gataagtctt ctgtaccggt gttaatcccc
     3301 cgtaatgagc agtttacaac caacgccggg gaaatcagag ttgatgaagt gctgccctct
     3361 gatgctaaaa aggaggagcc gcctccctgt accttcaccc tctgccggga tatctggctg
     3421 ctgcccgttc gcctggagca gattgaaaac cgcagtacga cccgtaatgg tgttatcaac
     3481 atcacctttt cggtcgcacc gggaacggac ttccgcacgc tggatctgaa caaacttcgc
     3541 ttctggctcg gcaatgacga caactatacc cgtgaccagc tttatttatg gttctgcgaa
     3601 tacttgcagg gtgccgacct gactatgggt gaacagcata ttcgcctgcc tgagtttatg
     3661 ctaaaagctg tcggttttga gccgcaggat gccatgctgc cctggccgaa aaacgtccac
     3721 agcggctacc ggatccttca ggagtatttc tgttaccccg atgcgtttct cttttttgat
     3781 ctttgtggtt gtccggcttt gcctgacgga ttgcaggcgg aattctttac cctgcaactg
     3841 cgtttttcgc gccctttgcc cgtggacatc cggctgcgcc gcgattccct gcgcctgtat
     3901 tgcgcacctg ccattaattt atttatccac catgcagaag ccatcacgct ggacaaccgg
     3961 cgggcagact atccgctggt tcccagccgc cattacccac aacattacga tgtattttcc
     4021 gttaacagtg tggtgagcca ggtccaggat atgttcagga aaaaagatct ggggcgtcct
     4081 gtttcgacgc aggccgcgcg ccagtggcca gcctttgaaa gtttcagcca tcagatggaa
     4141 tacagccgga agcgggaagt ggtgtactgg catcaccgga ccaaaacatc cctgttccat
     4201 cgcggctttg atcataccct tgcctttata catgctgatg gcagttatcc gtcagacgaa
     4261 tctctgctca gtaatgaagt ggtttcggta tcgctgacct gtaccaaccg tgagcttccg
     4321 tcacaaattc gttccggcga tatcaccggc acaaccggta aaaatgcggc tgttgcttcc
     4381 tttcgcaaca ttacccgccc gacgcaacca ctctggccgg tcattgatgg cagcctgcac
     4441 tggtccctgc tctccgccat gaacctgaat tatctgtcat tactggatac ggacgcgctg
     4501 aagcaggtca tcgctaactt tgatcgccac gcaatccacc atccgcagac ggcgcggctg
     4561 tcacaacaaa agctggatgc cattgagcgt ctggagaccc gccccgttga tcgcctgttt
     4621 acgggtattc ccgtccgggg gctggcctcc acgctgtatc tgcacccgga gccgtttgtc
     4681 tgtgaagggg aaatgtatct gctcggtacg gtgctttcgc attttctgtc gctgtacgcc
     4741 agcgttaact cattccacat gctgaccgtt gtgaacacag aaagccagga gacatggaaa
     4801 tggacggaaa gaatcgggca gcatcctctt atctgagtcc gggaaatccc ccggcagata
     4861 aagagcagaa tgatccgctg gcacaggttt tccataatgc ctgctcttac aatttttttg
     4921 cgatggcgga gctgttgcac cgcctggcaa agggtgaaaa ggggacgcca gaattatccc
     4981 tgcgcgacga tccggcacag gaaaccctgc gtttcagcgc cgatgccagc cttgccttcc
     5041 cgtgcagtga tatcagtgcg ctgatacggg atacgtcagg ggcattcagg atgaccacca
     5101 cctttatggg gttacagggg agccagtcgc ctctccccgg ttattacctc gatcacctgg
     5161 cctggaaagc ggtacatgaa caaagtccgg taggtgattt tctggatatg ttcagccacc
     5221 gcctgaccca gtttgtctgg catatctggc gtaagtaccg ctatcacatc agttttcgta
     5281 acggcggtgt ggatgcgttc tcgcagcgca tgtactccct tgtcgggctg ggccaccgcc
     5341 agcttcgcga taagctggcg attaatcaca gcaaaatgct ggcgtattcc gggatcctgg
     5401 caaatccggg acgatccccg gaaattatct gtggcctggt gtcccactgc tttgatttgt
     5461 cagaagtgac gctgcaaaac tggcaacgtc gcaaggtgga tattgaaccg gatcagcaaa
     5521 atagtctggg gagctattct cttaaaaatg gcgaaaaact ggcgggaaga tcggtactgg
     5581 gcaactttgt tctgggtaca cgagtgcccg atctcagcgg aaaattccag ctcagtatta
     5641 ccagcctgac ccgaaaacag ttcctctctt ttctgccgtc cggcgaaaac ttcctgccac
     5701 tgacgatgtt cgtgtccttc atcctgcgcg atcagcttgc ctgggactta catctggggc
     5761 tggcgccgga gcaggtgggc gcaatgcgcc tgggcgataa caaaagtgcg ctgctgggct
     5821 ggaccagctt cctcggcaca ccggaagaac gaccctcagt caccatcagg gtacggtcgt
     5881 aaattatgat aatcacggag cagaatatgc cggaagagaa actacagacg ctttcattgc
     5941 aggtcattaa cggcagtgag ctggaaagcg ggcgggcggc gcgctgtctg ttcacacagc
     6001 agggaaatgt cggccatggc cccgaatgcc actggtcggt acaggatcgt cagcagagca
     6061 ttccggccca ggcttttacc gttatcctgc acgatggcac attttgtcta cgcccgcaga
     6121 cggcacaact gtggctgaat caggcaaaag tcacagcaac atcagacctg atacagttgc
     6181 gccagggcga tgagatccag atcggacggc tgatggtgag ggttcatctg aaccggggag
     6241 atattcccca ttacgatgag gaaatggcca ctcccgaaac catcgttacc aatcgcgata
     6301 tgctcacgga taccctgcta tcaacggagg gtgcgccaca ctatccggga atgactcacc
     6361 ggcaccagct tgcagacacc gtggtaaacg gtttttctgc cgatccactc caggcacttc
     6421 agtccgaaag cctgattacc acgggcgatc cgctttcagg cattgcggct gtccggccat
     6481 cggcaccgct gtccgatccg gcaagtaatg gggggatcaa tactccgttt atggatctgc
     6541 cgcccattta tgccagccct ggcgatcata atgatgacat ctctgcggca gaaatggcgc
     6601 aacgccacct tgcggtcacc cccttactgc gcggtcttgg cggctcgctt accgtgagca
     6661 attccgacga tgcggatgat tttctggagg aggccggacg aacgttacag gccgcaataa
     6721 aaggtctgct cgatttgcag cagcagcgta acagcctctc agacaaacat ttgcgcccgc
     6781 tggaagataa cccgctgcgc ctgaacatgg attacgccac cgcgctcgac gtgatgtttg
     6841 ccgaaggtaa aagcccggta catctggcgg ctcccgccgc cgtcagtgaa agcctgcgca
     6901 atgtccgcca ccacgaagaa gctaaccggg cggcgattgt ggagtcgctt cgtgtcctgc
     6961 tggacgcctt ctcaccacaa aatctgctgc gccgctttgt gcagtaccgc cgcagccatg
     7021 aactgcgcca gccgctggat gatgccggag catggcaaat gtacagccat tattacgaag
     7081 aactggcctc cgatcgccag caggggtttg agatgctgtt taacgaggtc tacgcccagg
     7141 tctatgaccg ggtgcttcgt gaaaaacagc gggagccgga agcatgaaca ggaaggcgtt
     7201 tctggcctgc gtgctgatgt gcgtcatatt aaccggctgc gaaacggcga aaaaaatcag
     7261 cgaggtgatc aaaaacccgg atatccaggt cgggagcctg aaggaacaac cttcggaaat
     7321 aaccgtcacc ctgctgactg aaccggacac caataccaat gcggagggtg aatcagccgc
     7381 cgtggatgtg caactggtgt acatgactga cgactcaaaa ttgcaggcag ccgactatga
     7441 ccagatagcc agtacgccgc taccggatgt gctgggtaaa aactatatcg accaccagga
     7501 tttcaacctg ttgccggaca ccatcaaaac cctgccgccg gtaaagctgg atgagaaaac
     7561 gcagtttatc ggtgtcgtgg cctatttctc cgatgaccag gccactgaat ggaaacaaat
     7621 cgagacggtg gaaggaaccg gccaccatta ccgcctgctg gtgcatgtac gtcaaagcag
     7681 catcgaaatg aaaaaagagg atgaataatt atggcaacca cccgcaacaa ggtgatgtgg
     7741 caggaaggga tgctgatgcg cccacaccat ttccagcaac agcagcgtta caacgactac
     7801 ctggataacc agcgtttccg ggccatgaat gatttatcct ggggatttac cgaactcacc
     7861 ctcaacaatg aactgctggc gcagggtaag atcatgattg acagcgcgtc aggcacactg
     7921 cctgacggca ccgtcttttc tatccccgac caggacgcac tgcccgatcc gctgcacccg
     7981 caaaattttc cggacgaaag aagccgcaat atttacctcg ctctgccggt cgccagtgat
     8041 gtgagaaatg aaatcagcga cgggcggcga atcgggcgtt accggctgaa ttatgccgat
     8101 gtccgggatc tgcattcaga agaaggcgac gcgcgaacac tgacgctggg acaactgacg
     8161 ccgcgcatta tgagcggtgc agaagatatg agtgcctata ttacgctccc gctttgccgt
     8221 atcagcgatc gccatgccga cggctccctg acgctggatg acgattttat cccctcctgc
     8281 cagaatattc aggtcagtaa gaaactgcgt gtttatctca aagaggtaca gggggccatt
     8341 ggcggacggg caagcgatct ggcaaaccgc attggctctc cggcgcagag cggcatcgcg
     8401 gatgtggcgg aatttatgat gttgcagttg cttaaccgta accagacccg gtttacccat
     8461 cgcgcccgcc gatcccagct ccacccggaa gatttctacc ttgatcttgc cgggttgctg
     8521 ggtgaactga tgacctttac cgagccgtcg cgcctgccct gcccgcttga tgtgtatgat
     8581 catcatgacc tgaccaaaac atttaaaaca ctgttaccgg aagtcaaacg ggcgctgcat
     8641 accgtactgt cgccaagagc ggtcaatctg ccgctgcatc tgcgtgacgg catctggcag
     8701 gccgatgttc atgactcgga actgctacag tctgccacct ttgtactggc cgtggcggca
     8761 aatatgcctg tcgatcagat ccagcgtcag tttatccagc agtcgaaaat ttcctcgccg
     8821 gaaaaaatcc gcaatatggt cagtgtgcag atccccggca ttccattgcg tgccctgatg
     8881 gtggctcccc gtcagcttcc ttaccattcc gggttcagct atttcgaact cgacaagagc
     8941 ggacaggcct ggacagaaat ggctgccgcc ggggccgttg cactgcatgt atccggcagt
     9001 ttcccggatc tgaacatgca actgtgggcg ataagagggt aagacgatgg atgaggggtc
     9061 actgtctctg ccaccattta ccggatacga cgaaaaaggc cagcgcgact accatctcgc
     9121 gcttcgtggc aacagcctga atccgatgat cgacgcagcc acaccgctac tcggtatggt
     9181 aatgcgcctt tccaccatga acagccagac gatgccggag catctttttg ctcaggtggt
     9241 gacggatgtt caggccgtgg agcaactgct tcaggaacag ggctacgaac cgggcgtgat
     9301 tatctcgttt cgctacatcc tctgcacgtt tattgatgaa gcggcgctcg gtaacggctg
     9361 gtcgaacaag aacgagtgga tcaagcagtc attgctggtc catttccaca acgaagcctg
     9421 gggtggcgag aaagttttca tcctgctgga gcgcctgatc cgtgaaccca aacgttacca
     9481 ggatctgctg gaatttctct ggctctgttt ttctctggga tttcgcgggc gctacaaagt
     9541 ggcagcacag gatcaggggg aatttgagca gatctaccgc cgtctgtacc acgttctgca
     9601 taaactgcgc ggcgatgccc ctttcccgct gttacaccag gacaaaaaaa cccagggcgg
     9661 gcgttatcag ctcatcagtc gcctgaccat taaacatatt ttttgtggtg gcgttgtcgt
     9721 gctggcgttg ttttacctgt tctatctgtt gcggctggac agccagacgc aggacatcct
     9781 gtaccagcta aacaaattac ttcgataagg acgtttatga tccagattga tcttcccacg
     9841 ctggtaaaac ggctgaacct gttctcccgc caggcgctgg agatggccgc ctcagaatgt
     9901 atgagccagc aggcagcgga aattaccgtc agccatgtgc ttattcagat gctcgccatg
     9961 ccacgcagtg acctgcgggt tatcactcgg cagggcgata ttgacatgga agagttgcgc
    10021 caggcgctga cggtggagaa ctacacaacc gcccgttctg cggacagcta cccggcgttt
    10081 tccccgatgc tggttgagtg gctgaaagag ggctggctgc tggcgtcggc tgagatgcag
    10141 cacagcgaac tgcgcggcgg cgtgttgctg ctggccctgc tgcattcgcc gctgcgttat
    10201 atactgcctg ctgccgcccg gctgttgacc ggtattaacc gtgaccgtct gcaacaggac
    10261 tttgtgcagt ggacacagga gtcggcggaa tcagtcgtgc cggatgcaga cggtaaaggc
    10321 gcaggcacac tgacggacgc ctctgacacc ctgcttgccc gctacgccaa aaacatgacc
    10381 gaagatgccc gtaacggcag gcttgacccg gtactgtgcc gcgaccacga aatcgacctg
    10441 atgatcgaca ttctctgccg ccgccgtaaa aacaacccgg tggtggtggg cgaagcgggc
    10501 gtgggcaaaa gcgcgctgat tgaaggtctg gcgctgcgca tcgtggcagg tcaggtgccg
    10561 gacaagctga aaaacaccga tatcatgacc cttgacctgg gcgcattgca ggccggggca
    10621 tcggtgaagg gcgaattcga aaaacgtttc aaggggctga tggcggaggt catttcctct
    10681 ccggtgccgg tcattctgtt tatcgacgaa gcgcataccc tgattggcgc gggcaaccag
    10741 cagggcgggc tggatatctc caacctgctc aaaccggcgc tggcgcgcgg cgagctgaaa
    10801 accatcgccg ccaccacctg gagcgagtac aaaaaatact tcgagaaaga cgccgccctg
    10861 tcgcgccgct tccagttggt gaaggtcagc gaacccaacg ccgccgaagc caccattatt
    10921 ctgcgtggtc tgtcggtagt gtatgaacag tctcacggcg tgctgattga tgatgacgcc
    10981 ttgcaggccg cagcaacgct gagcgagcgt tatctctccg ggcgtcagtt gccggacaaa
    11041 gcgattgatg tgctggatac cgcctgcgcc cgtgtggcca tcaacctgtc atcgccacca
    11101 aagcaaatct cggcgctgac caccctgagc caccagcagg aggcggaaat tcgccagctt
    11161 gagcgcgagc ttcgcatcgg gctgcgtacc gacacctcac ggatgaccga ggtgctggtg
    11221 cagtatgatg aaacgctgac ggcgctggat gaactggaag tcgcctggca tcagcagcag
    11281 acgctggtac gggaaattat cgcgcttcgc cagcagttac tgggtatggc agaggacaca
    11341 gatgccgtag tggatacgtc gtcagagtca gagtcagagt cagagtcaga gtcagaacag
    11401 gataataccg ctgccatacc ggctgatgat gctggcagcg tacagccgga agaaaccgct
    11461 gaaacagttt ccccggtaca gcgactggca cagcttactg ccgaactgga cgccctgcat
    11521 aacgaccggt tgctggtttc cccgcacgtc gataaaaaac agattgcggc ggtgattgcc
    11581 gaatggaccg gcgtaccgct taaccgcctg tcgcagaatg aaatgtcggt catcaccgac
    11641 ctgccagtat ggctgggcga caccatcaaa ggccaggatc tggcgattgc cagcctgcac
    11701 aaacacctgc tgactgcacg cgccgacctg cgtcgtccgg gacgtccgct tggcgcgttc
    11761 ctgctggctg gccccagcgg tgtgggtaaa accgaaaccg tcctgcaact ggcggagctg
    11821 ctctatggcg gtcgccagta cctgaccacc atcaatatgt ccgagtttca ggagaaacac
    11881 actgtctcgc ggctgattgg ttcccctcct ggctatgtcg gctatggcga aggcggcgta
    11941 ctgaccgaag ccatccgcca gaaaccgtac tcggtggtac tgctcgatga agtggaaaaa
    12001 gcgcaccctg atgtgctcaa cctgttctac caggcgttcg acaagggcga gatggcagac
    12061 ggcgaaggcc gcctgattga ctgtaagaat atcgtcttct tcctcacgtc caacctcggc
    12121 taccaggtga ttgtcgaaca cgcggatgac cccgagatca tgcaggaagc cctctatccg
    12181 gtgctggcgg acttctttaa acctgccctg ctggcgcgaa tggaagtggt gccgtacctg
    12241 ccgctgtcga aagagacgct cgccaccatc attgccggga aactcacccg tctggataac
    12301 gtgctgcgca gccgctttgg cgcagaagtg attatagagc cggaagtgac ggacgaaatc
    12361 atgagccgtg tcacccgcgc ggaaaacggc gcaaggatgc tcgaatcggt catcgatggc
    12421 gacatgctgc cgccgctctc gctgctgctg ttgcagaaaa tggcggccaa tacggcgatt
    12481 gcccgtatcc gcctgtcggc ggcagacggc gcgttcacgg cagatgtgga agatgctcag
    12541 gacgaccaat ccgtcacaga ggatgaaccg gttttatgag caggatgcgc gttttccggc
    12601 aaacaggatg gtttatcgcg ggcctgatgg cgggcttacc cgccagtgcc gcgcccgcag
    12661 aaacatcgtc aatggccggt gtggcggtag ccgttgccac agcgaccccg ctggatgcgg
    12721 cagccacgtt acaggccatg cagtcctgtc ggcgggagtc tgcggcgctg gagcggcttg
    12781 actgctatga ccgcctgctg gaaccgctgt ccccgtcagg gtttgacggg gcgctggtca
    12841 aagctggttt tgtgggcgaa gcctggaccc gcgccacaga gcaggaaaaa cgccgccagg
    12901 gcaacaccac agagctgctg gtgacgcagg tgccggggga acgcccgacg gtggtgataa
    12961 ccaccccggc tatcggccat gttccgccac gcccggtgct gatgttcagt tgcgtggaca
    13021 atattacccg gatgcaggtg gcgctgatgc accctctgga tgtccatgat atcgccgtca
    13081 ccctgaatgc cgacagccgg gcattacgca gccactggtt tgtgcgcgag aacggcaccc
    13141 tgctggagtc cagccggggg ttatccggca ttgatgaaat taagcaactg ttcggcgcaa
    13201 aaacgctgac ggtggatact ggcgcagaca acgccgccgg aaagctgacc tttaacattg
    13261 acgggctagc aagggcgatt gctcccctgc gcgatgcctg ccactgggcg ggagaataaa
    13321 aatatggcaa tggacttacg cgatccgaat gtctggatat cgcacctgct ggaaaacctg
    13381 ccggatgaga aactggcatc ggcgttgaaa gacgacaacc cggactggga gtatatcgac
    13441 ggcgaaatcg tcaaactggg gtcactggcc cacagccagc tggatatccc ggaactccag
    13501 cgccgggggc tggtcattct ggcttcggag agtaaggatt ttcgtctgct ggcgcatctg
    13561 ctgagaaccc tgcaacacgc cggtgatcct catctcgcat tgcgcctgct ggcgctgtat
    13621 gtggagcatt actggtcggt ggctgcgccg cagaatatgg cacataaaaa acgctttgcc
    13681 agccaggtca ttaaacgctt tgaaacaggt attgaaggct tttcacagaa cgccgccaca
    13741 gcgcagcgcg acgcgctgtc aggggaactg gcgaaactgg cgcagtgctg gcagtcacat
    13801 aacgccccgg aactggcact ggctaccgat gatctttttg ccctgtacca gcgtgcgttt
    13861 aatcgtgcgg caccggctcc tgcccccact ccggcggcct ccgatagttc accacagacc
    13921 accgccacgt ctgaaagcgg cgtgacgcaa cccagtactc cgactcccca aatcgccatc
    13981 gacagtcacg acgacaaagc ctggcgcgac acgctgttaa aagtggcggc tattttatgt
    14041 gaacgccagc cggactcgcc gcagggctat cgcctgcgcc gccatgccct gtggcaatcc
    14101 atcaccagta caccccaggc ggaaagcgat ggacgtaccc cattggctgc ggtctctgcc
    14161 gatatggtgg cggattacca gtcccggctt gccagcgcgg atatgacgct gtggcagcag
    14221 gtggaaaaaa gcgtactgct ggcaccctac tggctggacg gtcactgcat ttctgcacag
    14281 acggcgcacc gtcttggcta taccgctgtg gccgatgcca tccgcgatga agtcgtttcc
    14341 tttcttggcc gtctgcccgc gctcgctgat cttctgttta acgaccacac accgtttgtt
    14401 tctgaaaaaa cgaagcaatg gcttgcaact cagccaggta accaggccgc gccagtcata
    14461 caggtgagta atgaagatat tcaggctgcg cggttgtgtt ttgatgaaca ggggctggaa
    14521 gccgccttac ggtatctgga tacgttgccc gccggtgaac cccgcgacca gtttcaccgg
    14581 cagtattttg gcgcgcagct tatggaagag gcggggctgg tacagcttgc gcagcagcag
    14641 taccggatgt tgttcaggat gggattgcag atgatggtgg cagactggga gccgtccctg
    14701 cttgagcagc tagaacaaaa attcacggca gaacaataag cgataacagg agtttttgtg
    14761 ttcagattac caacaccccg actactcagc ggactcaaat cagccctacg cccggcgatg
    14821 ccccggttta aagtctccgc tttctggctg ctgatactgg cgtggatttt tctgctggta
    14881 tggatctggt ggaaaggccc gacgtggacg ctgtatgaag aacagtggct caaaccactg
    14941 gcgaaccgct ggctggcaac ggcggcatgg gggattatcg ccctggtgtg gcttaccgtc
    15001 caggtgatga agcgcctgca acaacttgaa aaaatgcaaa agcagcagcg cgaggaggcc
    15061 attgatccgc tcagtgtgga gctgaacgcc cagcagcgtt accttgaccg ctggctgctg
    15121 cgcctgcaac gccatctcga caaccgccgt ttcctgtggc agttgccgtg gtatatggtc
    15181 atcggcccgg cgggcagtgg caaaacgaca ctgctgcgcg aggggtttcc gtcagacatt
    15241 atttatgccc cggagggcgc gcgcggcaca gaacaacgcc tgtaccttac gccccatgtc
    15301 ggtaaacagg cggttatctt tgatatcgac ggcacactct gcgctcccgc tgatgcggat
    15361 atcctgcatc gccgtctgtg ggaacatgcc ctcggctggc tgaaagaaaa gcgcgcgcgc
    15421 cagccgctga acgggattat tctgacactc gatttacccg atctgcttac cgcagacaaa
    15481 cgccgccgcg agcatctgtt acagacgctg cgcagccgct tgcaggatat acgccagcat
    15541 cttcactgcc agttaccggt ttacgtggta ctcacccggc ttgatttatt gcagggtttt
    15601 gccgccctgt tccagtcact gaacagacag gatcgcgatg cgattctggg cgtcacgttc
    15661 acccgccgcg cccatgaaaa tgatgactgg cgaacagagt tgaatgcttt ctggcagaca
    15721 tgggtggatc gaatgaacct agcgttgccg gatctgatgg tcgctcagac tcacacccgc
    15781 gcgtctttat ttagtttttc ccgccagatg cagggaagcc gtgaaccgct ggtgtcactg
    15841 cttgagggtc tgcttgatgg cgaaaatatg aacgtgatgc tgcgtggtgt ctatctcacc
    15901 tcttcgcttc agcgtggaca gatggatgat atattcaccc agtctgccgc ccgccagtac
    15961 cggctgggca ataacccact ggcttcctgg cctctggtgg acaccgcgcc ttatttcacc
    16021 cgcagcttgt tcccgcaggc attactcgca gagcctaatc tggcaacgga gagccgcgcc
    16081 tggctgatgc gttcccgtcg ccgcctgacg gttttctcta ccacaggagg cgtggcggca
    16141 ctgctgctca tcaccggctg gcatcactat tacaacggta actatcagtc cggcatcacc
    16201 gtgcttaagc aggccaaagc ctttatggac gtgccgcctc cgcaggggga agatgactac
    16261 ggcaacctgc aactgccgct gctgaacccg gtacgtgatg ccacactggc ctatggcgac
    16321 tggggcgacc gcagccgtct ggccgatatg ggactgtacc aggggcgacg tatcgggcca
    16381 tatgtggaac agacctatct gcaactgctg gagcaacgtt acctaccctc gctgtttaac
    16441 gggctggtta aagcgatgaa tgccgcgccg ccggagagtg aagaaaaact cgcggtgctg
    16501 cgcgtgatgc gaatgctgga ggacaaaagc ggacgtaaca atgaggtggt gaagcagtat
    16561 atggcaaaac gctggagcga aaagttccac ggccagcgcg atatccaggc acaactgatg
    16621 tcccatcttg actacgcgct ggctcatact gactggcacg cagagcgtca ggcgggcgac
    16681 ggtgacgcca tcagccgctg gacgccatat gacaagcccg tggtatcagc acagaaagaa
    16741 ctgagcaaac tgcctgtcta ccagcgggtt taccagagcc tgaaaacgcg ggcgctgggc
    16801 gttcttcctg ccgacctcaa tctgcgtgac caggtagggc caacctttga tcaggtattt
    16861 acatctgccg atgacaacaa actggttgtt ccacagtttc ttacccgtta cggcctgcaa
    16921 agctattttg taaaacagcg cgatgaactg gttgaactga cggcgatgga ttcctgggta
    16981 cttaacctta cccgcagcgt gaaatacagt gacgccgacc gcgcggaaat ccagcgccag
    17041 ctcaccgagc agtatatcag cgactacacc gccacctggc gggccgggat ggacaatctg
    17101 aatatccgca attttgagtc catcggacaa ctgaccgggg cgctggagca ggttatcagc
    17161 ggcgaccagc ctttgcagcg ggcgctgacc gtgctgcgtg acaacacaca gccaagcgtc
    17221 ttttctgaaa aactctctgc caaagaacgg gatgaggccc tggcagagcc ggactaccag
    17281 ttactcaccc gtctcgggca tgagttcgcc ccggaaaaca gtaccctggc agtacagaaa
    17341 gacaaagaaa gcacgatgca ggcagtatat cagcaactca ccgagttgca ccgctatctg
    17401 ctggcgatcc agaacgcgcc tgtaccaggg aaatcggcgc tgaaagccgt gcagttacgg
    17461 cttgatcaga acagcagcga tccgatattc gccacccgcc agatggcaaa aacgctgcct
    17521 gctccgctca accgctgggt tggcaggctg gctgaccagg cctggcatgt ggtgatggtg
    17581 gaggccgttc actatatgga agtggactgg cgcgacagcg tggtgaaacc gtttaacgag
    17641 caactggcaa ataactatcc gtttaatccg cgttctgcac aggatgcctc actggatgcc
    17701 ttcgaacgct tctttaaacc ggatggcata ctggatacct tctaccagca gaacctgaag
    17761 ctgtttatcg ataatgacct gagtctggag gatggcgata acaacgtcat tattcgcgaa
    17821 gatattattg cgcaactgga aactgcgcag aaaatccgtg acatcttctt cagcaaacag
    17881 aacggtctgg gaacatcctt tgccgtggaa acggtatcgc tttcaggcaa taaacgccgc
    17941 agtgtactga accttgacgg tcagttagtc gattacagcc agggccgtaa ctataccgcc
    18001 catctggtct ggcctaacaa catgcgcgaa ggcaacgaaa gtaagctgac gctcatcggc
    18061 accagcggca acgcgccgcg cagtatcagc ttcagcgggc cgtgggcgca gttccgcctg
    18121 ttcggggccg gacaactgac cggagtacag gatggcaact ttaccgtgcg ctttagcgtg
    18181 gacggtggcg cgatgaccta ccgtgtgcat accgacacgg aagataaccc gttcagcggt
    18241 gggttgttca gccagtttgg tctgtcagac acactgtact gaggaggcag ggatgaatag
    18301 taacgtactg acacaaacta tcgttaccgg cagtgacccg cgcgggctgc cggaattcag
    18361 cgccatccgc gaggaaataa acaaagccag ccacccgtca cagcctgagc tgaactggaa
    18421 actggtggag tcgctggcgc tggcgatttt taaagccaac ggtgtggatt tacacaccgc
    18481 cacctactat acgcttgccc ggacacggat acagggactg gcgggattct gcgaaggtgc
    18541 ggaactgctg gcggcaatgg taagccacga ctgggataag ttctggccgc agggcggccc
    18601 ggcgcgtact gaaatgctgg actggtttaa ctcccgaacc ggcaatattc tgcgtcagca
    18661 aatctccttt gcggaatctg atctgccgct gatctaccgc acagagcggg cattgcagct
    18721 tatctgcgac aagcttcagc aggtagagct gaagcgcgtt ccgcgcgtgg agaatctgct
    18781 ctattttatg cagaacacgc gtaaacggct tgaaccccag ctgaagagta acactgagaa
    18841 cgccgcacag accacggtca gaacgctgat ttatgccccg gaaacacagc catcttccac
    18901 accagaagcg gtagtgcctc ccctgcccgg cctgcctgag atgaaagtgg aagtgcgcag
    18961 tctgacagag aatcccccac aggccagtgt gataaagcaa ggcagtacgg taagagggtt
    19021 tatcgcaggg atcgcctgtt cagtggctgt cgcctcagca ttgtggtggt ggcaggtcta
    19081 tccggtgcag cagcaactgt tacaggttaa cgacaccgct cagggcgcag caacggtgtg
    19141 gatggcctca cctgaactcg aaaactatga gcgcaggctg caacaacttc ttgatacctc
    19201 cccggtacag ccgctggaaa ccgggatgca gatgatgcgt gttgccgaca gtcgctggcc
    19261 ggaaagcctg caacagcaac aggcctcgac acaatggaat gaggcactca aaacccgcgc
    19321 acagaatagc ccgcagttgc gtggctggtt gcagacccgc caggacttac atgcttttgc
    19381 agatctagtg atgcagcgcg agaaagaggg actaaccctt tcctatatca aaaatgtcat
    19441 ctggcaggcg gagcggggac tggggcagga aacacccgtt gagtctctgt tgacgcagta
    19501 ccaggatgcc cgtgcgcaga agcagaatac agatgcgctg gaaaaacaaa ttaatgagcg
    19561 actcgaaggc gtgttaagcc gctggctgct gctgaataat aatacgatac cgacgataaa
    19621 aaaaggcatt gaatttcaat aacattcatg aatacaaagg agttttaaat ggcgaattta
    19681 atttatttaa cactaaatgg tgataatcaa gggctcattt caagtggttg ttcatcacaa
    19741 ccatccattg gaaataaagc tcaaacagca cacattgatc aaattatgat ttatgaattc
    19801 atgcatggat taaatcgaga tcaaaacgtc aaccatcatc atttgacaat aaaaaaacct
    19861 atagataaag catcaccctt attaggcaaa gcaatttgcg acaatgaatt attgacatgt
    19921 gatttttcct tttatcgtac aaataaattt ggaattaatg aactgtacta taaaatcaaa
    19981 ttaactggag caaagataag cgacattcat gtctctatat cacatattgt tgttgataac
    20041 agcgttcaac ccgaagagtc agtttctttc agttatgagt caattatatg ggagcattgt
    20101 tccgctggca ctagtgcata cagtttatgg gaagatcgtc ttttttaaaa acatatggga
    20161 ttattataat ggctaacaat attttacact cttcaacatc agacagtgaa agcaaaaata
    20221 caagcactga cagtacgtta tttaatgaaa aattcccatt tcaactaaga caagatttcg
    20281 catcaagaat aacagaagat gaaagtgatt tttttaaatg gaaaaaaata
//
LOCUS       DAZFKF010000091        20131 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFKF010000091 DAZFKF010000000
VERSION     DAZFKF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.091"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(568..652)
                     /locus_tag="ACHKA1_003847"
     tRNA            complement(568..652)
                     /locus_tag="ACHKA1_003847"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(616..618),aa:Leu,seq:caa)
     gene            848..1868
                     /gene="ahr"
                     /locus_tag="ACHKA1_003848"
                     /pseudo
     CDS             848..1868
                     /gene="ahr"
                     /locus_tag="ACHKA1_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
     gene            complement(1872..2435)
                     /gene="idnK"
                     /locus_tag="ACHKA1_003849"
     CDS             complement(1872..2435)
                     /gene="idnK"
                     /locus_tag="ACHKA1_003849"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO5681437.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPHADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            2652..3683
                     /gene="idnD"
                     /locus_tag="ACHKA1_003850"
     CDS             2652..3683
                     /gene="idnD"
                     /locus_tag="ACHKA1_003850"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFO5681438.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3707..4471
                     /gene="idnO"
                     /locus_tag="ACHKA1_003851"
     CDS             3707..4471
                     /gene="idnO"
                     /locus_tag="ACHKA1_003851"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFO5681439.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4534..5853
                     /gene="idnT"
                     /locus_tag="ACHKA1_003852"
     CDS             4534..5853
                     /gene="idnT"
                     /locus_tag="ACHKA1_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFO5681440.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            5920..6918
                     /gene="idnR"
                     /locus_tag="ACHKA1_003853"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             5920..6918
                     /gene="idnR"
                     /locus_tag="ACHKA1_003853"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFO5681441.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRTAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            6996..8498
                     /gene="yjgR"
                     /locus_tag="ACHKA1_003854"
     CDS             6996..8498
                     /gene="yjgR"
                     /locus_tag="ACHKA1_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO5681442.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASNEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8659..9741)
                     /gene="lptG"
                     /locus_tag="ACHKA1_003855"
     CDS             complement(8659..9741)
                     /gene="lptG"
                     /locus_tag="ACHKA1_003855"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO5681443.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9741..10841)
                     /gene="lptF"
                     /locus_tag="ACHKA1_003856"
     CDS             complement(9741..10841)
                     /gene="lptF"
                     /locus_tag="ACHKA1_003856"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO5681444.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(10904..10954)
                     /gene="ytgA"
                     /locus_tag="ACHKA1_003857"
     CDS             complement(10904..10954)
                     /gene="ytgA"
                     /locus_tag="ACHKA1_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO5681445.1"
                     /translation="MSIIILATISTTQENK"
     gene            11108..12619
                     /gene="pepA"
                     /locus_tag="ACHKA1_003858"
     CDS             11108..12619
                     /gene="pepA"
                     /locus_tag="ACHKA1_003858"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO5681446.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12973..13416
                     /gene="holC"
                     /locus_tag="ACHKA1_003859"
     CDS             12973..13416
                     /gene="holC"
                     /locus_tag="ACHKA1_003859"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO5681447.1"
                     /translation="MKNATFYLLDNDITVDGLNAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13416..16271
                     /gene="valS"
                     /locus_tag="ACHKA1_003860"
     CDS             13416..16271
                     /gene="valS"
                     /locus_tag="ACHKA1_003860"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO5681448.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEYN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16327..17523)
                     /gene="yjgN"
                     /locus_tag="ACHKA1_003861"
     CDS             complement(16327..17523)
                     /gene="yjgN"
                     /locus_tag="ACHKA1_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO5681449.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKRFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
     gene            17716..18219
                     /gene="yjgM"
                     /locus_tag="ACHKA1_003862"
     CDS             17716..18219
                     /gene="yjgM"
                     /locus_tag="ACHKA1_003862"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5681450.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPVIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(18265..18681)
                     /gene="rraB"
                     /locus_tag="ACHKA1_003863"
     CDS             complement(18265..18681)
                     /gene="rraB"
                     /locus_tag="ACHKA1_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO5681451.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            18843..19847
                     /gene="argF"
                     /locus_tag="ACHKA1_003864"
     CDS             18843..19847
                     /gene="argF"
                     /locus_tag="ACHKA1_003864"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO5681452.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSN
                     "
     gene            complement(19904..>20131)
                     /locus_tag="ACHKA1_003865"
     CDS             complement(19904..>20131)
                     /locus_tag="ACHKA1_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5681453.1"
                     /translation="TNEIVKKYPGYVVKIFEEKMGNELKIRTRMMETLRDGKINIYDY
                     INEKQLEKLNPPQDLRSAIEKIGWKNRPITA"
BASE COUNT         5179 a         4951 c         5126 g         4875 t
ORIGIN      
        1 ataatccgat attggtcgca tttaatggca tggtagcaaa gttttcatca catacctcct
       61 ttgcaccaga ataaacgcca gagatctgaa attgcgagta cactacagaa attgtactgc
      121 cggtcccatc agcataaaac catagttaca caggtaatca tcattattcg ccaagataat
      181 aaatattatt cggtagaata gcgttctcag gtggtcagta aaattgtctt caacaagatg
      241 tctgcgcatc tcgtctgcta gccctacata actgctctcg accgaaacta tttattgaat
      301 gttcaccaag gtgggcgtgg agacaaaatg acgtcttttg atattagcta cctgagccac
      361 atggtcaaaa cgttcaagta gcatagcgaa accaccctgc ccacgtaatg tggacgatgc
      421 agcttccagc cagagcaaac ttgggaccat cgactacaaa atgaagaata ctgaactgct
      481 tttctggcag gcctatatac tgcacggata tgggtgattt cagacacaaa aaaagccgcc
      541 cttgagcgac tcgatttgca tacggtgtgg tgccgaaggc cggactcgaa ccggcacgta
      601 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      661 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      721 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      781 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      841 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcaggcggcg aactggaagt
      901 ttatgagtac gctcccggtg agctgaagcc acaagatgtt gaagtgcagg tggattactg
      961 cgggatctgc cattccgatc tgtcgatgat cgataacgaa tggggatttt cacaatatcc
     1021 gctggttgcc gggcatgagg tgattgggcg cgtggtggca ctcgggagcg ccgcgcagga
     1081 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgtggtca
     1141 ctgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccgacgat
     1201 tatgaatcgc ggtggctttg ccgagaagtt gcgtgcggac tggcaatggg tgattccact
     1261 gccagaaaat attgatatcg agtccgccgg gccgctgttg tgcggcggta tcacggtctt
     1321 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1381 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1441 tagttctaat ccggcgaaag agcaggaagt gctggcgatg ggtgccgata aagtggtgaa
     1501 tagccgcgat ccgcaggcac tgaaagcact ggcggggcag tttgatctca ttatcaacac
     1561 cgtcaacgtc agcctcgact ggcagcccta ttttgaggcg ctgacctatg gcggtaattt
     1621 ccatacagtc ggtgcggttc tcacgccgtt gccggttccg gcctttacgc tgattgcggg
     1681 cgatcgcagt gtctctgggc tctgctaccg gtacacctta tgagttgcgt aagctgatgc
     1741 gctttgccgc ccgcagcaag gttgcgccga ctaccgaact gttcccgatg tcgaaaatta
     1801 acgacgccat ccagcatgtg cgcgacggta aggcgcgtta ccgcgtggtg ttgaaagccg
     1861 atttttgaaa atcattcgca gcgctgatct gaggcgctgc cctctttcgc acatattctg
     1921 ttttgtcgta tcgccagcac agcctgccga cattgttcgg tgacattggc aatatcatgg
     1981 ttgatatcaa tgcgcacaat atcctgttca tctgcatgtg gacgctccag tgcctcaaac
     2041 tgacttttta gtaacgctac cggcataaaa tgcccagccc gacgctgcat tcgcgcgaga
     2101 atagtttcat agtcgccatc taaccagagg aaatgaacat gggggctacc cttgcgtaaa
     2161 atatcacgat actgtttttt taatgacgaa cagacaataa atcctgtttc attcttttta
     2221 taaagactgt atgaagcatc atttaagcgt tccagccagg gaagtcgatc ttcatcagat
     2281 aatggaatac cctgcgacat tttatctata tttttggctg gatgaagatc gtcaccatca
     2341 ataaatttag cagataataa cgcggcaacc ttgctaccaa ttaatgtttt accactccct
     2401 gaaacgccca tcaaaataaa gctttcaccc gccatttaaa atctcctgaa tcatcttacc
     2461 tgctgcgata ctacgcctgg cggcataata atttcacgtt tttttatttc tgctgataag
     2521 aattacaagg cacatcacgt tatgcgtaac atagtaatgt aacaattttc tgacgtgatc
     2581 ttcatcacaa ataatgacaa ttaaaaccgc ttaaatgctt ccgaggtgtc tacctgacca
     2641 gtgaggaatt tatgcaagtg aaaacacagt cctgcgttgt tgcgggcaag aaaactgttg
     2701 ccgttaccga gcagacgata gattggaata ataatggaac attagtacaa ataacccgag
     2761 gtggaatttg cggttccgat ttacattatt atcaggaagg aaaagtaggt aatttcatga
     2821 taaaggcacc gatggtgtta ggtcatgaag ttatcggtaa agttattcat agcgactcat
     2881 cagaattaca tgaagggcaa acggtagcca ttaatccgtc taaaccgtgc ggtcactgca
     2941 aatactgcat tgaacataac gagaatcagt gtacagatat gcgttttttt ggcagtgcca
     3001 tgtatttccc tcatgttgat ggtggtttta cccgttataa aatggtcgaa acgtcgcaat
     3061 gtgtccctta tccggccaaa gctgacgaaa aggttatggc ttttgccgaa cctttagccg
     3121 tcgcgattca tgccgcacat caggccggcg agttacaggg caagcgagta tttatttccg
     3181 gtgttggacc cattggctgc ctgattgtca gtgcagtgaa aacactgggg gccgcggaaa
     3241 ttgtctgtgc tgatgtgagt ccccgttccc tttcgctggg caaagagatg ggggcggatg
     3301 tgctcgtaaa cccacaaaac gacgacatgg atcactggaa agcggaaaaa ggctatttcg
     3361 atgtcagctt tgaagtgtcc ggtcatcctt catcagtgaa tacctgtctg gaggtcactc
     3421 gtgcacgcgg cgtaatggtg caggtaggta tgggaggcgc gatggcagaa ttcccaatga
     3481 tgacgttgat tggtaaggag atttcactca gaggctcttt ccgttttacc agcgaattta
     3541 ataccgcagt gtcatggctg gcgaatggcg ttatcaatcc actgccttta ctgagtgctg
     3601 aatatccctt cactgacctg gaagaggcgc tacgtttcgc cggtgataaa acccaggcag
     3661 caaaagtcca gcttgttttc taagagataa ataaaggaat aatacaatga acgatctatt
     3721 ttcactggca ggaaaaaata tcttgattac cggttcagca cagggcattg gctttttact
     3781 ggcaaccggc ctgggtaaat atggcgcaca aataattatt aatgatatta ctgccgaacg
     3841 cgcagaactt gctgtagaaa aactccacca ggagggtatt caggccgttg ccgcaccttt
     3901 taatgttact cataaacatg aaattgatgc cgccgttgaa catatcgaaa aggacatcgg
     3961 ccccattgat gtgctggtga ataacgccgg tatccagcgc cgtcatcctt ttactgagtt
     4021 ccctgaacaa gagtggaatg atgtgatcgc agtaaaccag accgccgtgt tcctggtatc
     4081 gcaagcggta actcgtcaca tggttgaacg caaggcaggt aaagttatta atatttgctc
     4141 gatgcaaagc gaactgggac gtgacaccat caccccttat gccgcatcga aaggggcggt
     4201 aaaaatgctc acccgcggca tgtgcgtcga gctggcgcgc cacaatattc aggtcaacgg
     4261 tattgcgccg ggctatttca aaacagaaat gactaaagca ctggttgagg acgaagcctt
     4321 caccgcctgg ttgtgcaaac ggacccccgc cgcacgctgg ggagatccgc aggaactgat
     4381 tggtgctgcg gtgttccttt cttcaaaagc ctctgatttc gtaaacggcc acctgttgtt
     4441 tgttgatggc ggcatgttag tggctgttta acagcccccc ctacgtgcag cgcgaaagcg
     4501 ctgcttttct ggcactaata agagataaag actatgccat taatcattat tgcggcaggc
     4561 gtcgcgctgc ttcttatcct gatgatcggc tttaaagtta acggctttat tgccctcgtt
     4621 ctggtagctg ccgtcgtcgg atttgccgaa gggatggatg cacaggccgt cctgcactct
     4681 atacaaaatg gtatcggcag cacgctcggc gggctggcaa tgatcctcgg tttcggggcc
     4741 atgttaggca agctgatttc tgatacgggt gcggcacaac gtatcgccac tacgctgatt
     4801 gctacttttg gtaaaaaacg cgtgcaatgg gcgctagtga tcaccggtct ggttgtgggc
     4861 ctcgccatgt tttttgaagt gggttttgtc ctgctgttgc cgttggtatt taccatcgta
     4921 gcatcatcag gattacccct gttgtatgtt ggcgtaccaa tggtagcagc gctctctgta
     4981 acccactgtt ttctgccgcc acatccaggg cctactgcca tcgcgactat ctttgaggct
     5041 aatctcggaa cgactttact gtatggattt atcattacca ttccgacagt tattgtcgca
     5101 ggaccgctgt tttctaaact gctaactcgc tttgagaaag caccaccgga aggcttattt
     5161 aatcctcatc tgtttagcga agaggagatg ccctccttct ggaacagtat tttcgctgcc
     5221 gtgatcccgg tcatcctgat ggctatcgcc gccgtttgtg aaattacgtt accgaaaact
     5281 aacaccgtgc gcctcttctt tgaatttgtc ggtaaccctg ccgttgcgct gtttattgcc
     5341 attgttattg cgattttcac actgggccga cgtaatggac gcaccatcga gcaaatcatg
     5401 gatatcattg gggattctat aggcgctatc gcgatgattg tgtttattat cgctggcggc
     5461 ggcgcgttta agcaggtatt agtagatagc ggtgtcgggc agtatatttc acacttaatg
     5521 accggaacta cgctttcgcc gttattgatg tgctggactg ttgcggcgct gttgcgtatc
     5581 gctctgggct ctgccaccgt cgcggccatt accaccgcgg gtgtggtgtt gccgataatc
     5641 aacgttaccc atgccgatcc cgctttaatg gtactggcaa ccggtgcggg cagtgtgatc
     5701 gcgtcacacg taaacgaccc tggcttctgg ctatttaaag ggtattttaa tctgacggtt
     5761 ggtgaaacgt tgcgtacctg gacggtgatg gaaaccctta tttctattat gggtttgctg
     5821 ggcgtgttag ccattaacgc cgtattgcac tgaaagaata aaatattgtg atgtagtttc
     5881 gggggaagat tgaagattcc cccgacgttg ggagaagtta tgcgcaatca cagaatttct
     5941 ttacaggata tcgctacgct ggctggcgta acaaaaatga ccgtgagtcg ttatatccgc
     6001 tcgccgaaaa aggtggcaaa ggaaacaggc gagcgcatcg ccaaaattat ggaagagatc
     6061 aattatatcc ctaatcgcgc tcccggcatg ttgttgaatg cacaaagtta taccctcggt
     6121 atcttgattc cgtcatttca aaatcaactc ttcgctgaca ttctcgcggg aatagaatcc
     6181 gtcacctctg agcataatta tcagaccctt attgctaatt acaattacga tcgcgattcc
     6241 gaagaagagt cggtgattaa cctgctctct tataacattg acgggattat actttcggaa
     6301 aaatatcaca ccatcaggac ggtgaaattt ctgcgttcag ccaccattcc ggtcgtggaa
     6361 ttgatggatg tacagggaga acggctggat atggaggtcg gttttgataa tcggcaggcc
     6421 gcttttgaca tggtgtgtac catgctggag aagcgagtca ggcacaaaat tctgtatctc
     6481 ggttccaaag atgacacccg cgatgaacag cgttatcagg ggtattgcga tgcgatgatg
     6541 ctgcataatc tttccccatt acgcatgaat ccacgcgcca tctcatccat tcatttagga
     6601 atgcagttaa tgcgtgatgc ccttagcgca aacccggatt tagacggtgt attttgcacc
     6661 aatgacgata ttgcgatggg tgcgcttctg ctgtgccgcg agcgaaacct cgccgtgcca
     6721 gaacaaattt ccattgcggg ttttcatggg ctggagattg gcaggcaaat gatcccaagc
     6781 ctcgccagcg taattactcc ccgttttgat attggtcgga cggctgccca aatgttgctg
     6841 agcaaaatta aaaataacga tcacaaccac aacactgttg acctcgggta tcagatttat
     6901 cacggcaaca cgctttaggt tattttctct tcattacacg tattctcttt gccggacgtt
     6961 gcctatactc caggcaggaa actggaggaa atctcatgag cgaacccctg ttaattgccc
     7021 gcacgccgga cacagaacta tttttactgc cgggaatggc taaccgtcac gggctgatta
     7081 ctggcgcaac ggggacgggt aaaaccgtta cgctgcaaaa actggcagag tcattgtcgg
     7141 aaatcggcgt gccggtgttt atggctgatg tgaaaggcga tctgaccggt atcgcgcagg
     7201 caggaacggc gtcggaaaaa ctgctcgcaa ggcttaaaaa tatcggcgtc aatgattggc
     7261 aaccgcatgc caatccggtg gtggtgtggg atatctttgg cgagaaaggc catccggtgc
     7321 gggcgacggt ttcggatctg gggccgctgt tgctggcgcg gctgttgaat ctcaacgatg
     7381 tgcaatctgg cgtgctgaat atcatcttcc gcattgctga cgatcagggg ctgttactgc
     7441 tcgactttaa agatctgcgg gcaattaccc agtacatcgg cgataacgcc aaatccttcc
     7501 agaatcagta cggaaatatc agtagcgcat cggttggtgc catccagcgc gggctgttgt
     7561 cgctggaaca gcaaggcgca gcacacttct ttggtgagcc gatgctggat atcaaagact
     7621 ggatgcgcac cgatgccaac ggtaaaggcg ttatcaatat cctcagcgcc gagaaacttt
     7681 atcagatgcc gaaactgtac gccgccagcc tgctgtggat gctttcagag ttgtatgaac
     7741 aattgccgga agcgggcgat ctggagaaac caaaactggt gtttttcttc gacgaggcac
     7801 atctgctgtt taacgatgcc ccgcaggtac tgctggataa gattgagcag gtgataaggc
     7861 ttattcgctc aaaaggcgtg ggcgtctggt tcgtttcgca aaacccgtct gatattccgg
     7921 ataatgtgct cggccagctc ggtaatcgcg ttcagcacgc tttgcgggct tttacgccaa
     7981 aagatcagaa agcagtgaag gcagcggcgc aaaccatgcg ggccaatccg gcatttgata
     8041 ccgaaaaggc aatccaggaa ctggggaccg gcgaggcgtt aatctcgttt ctcgatgcaa
     8101 aaggaagtcc ttctgtggtg gaacgggcga tggtgattgc gccttgttcg cggatgggac
     8161 cggtgacgga agatgagcgc aatggcttga ttaaccactc cccggtgtat ggcaaatatg
     8221 aggatgacgt ggaccgggaa tccgcctatg agatgttgca aaaaggcttt caggccagta
     8281 acgagcagca aaataatccc cccgcgaaag ggaaagaggt agcggtagat gacggcattc
     8341 ttggtggatt gaaggatatt ttgttcggca ctaccggacc acgcggcggg aagaaagatg
     8401 gtgtggtgca aacaatggcc aaaagcgccg ctcgccaggt gacgaatcag attgtacgcg
     8461 ggatgttggg gagtttgctg ggggggagaa aaaggtaagc caggtgcgat ttgtctttgc
     8521 ctgatgcgac gctggcgcgt cttatcatgc ctacaccact gcaatatatt ggattttcat
     8581 aatttttcag gaatgaacga agcacacttt gctaataata ataaagggag ctttcgctcc
     8641 ctttatgcgt tcattagatt acgattttct cattaacagc cacaggctga ttaagaagaa
     8701 gctggcgctt ggcaacagtg cgccaatgat cggcgggatg ccgtaaacca acgtcagcgg
     8761 accgaagatc tggtccagta cgtagaagac aaaaccgaag ctgataccgg tgaccacacg
     8821 cacgcccatc ggtacgctac gcagtgggcc aaagatgaac gacagcgcca tcagcatcat
     8881 caccgccacg gatagcggct ggaagatttt gctccacatg ttgagctgat aacgtccggc
     8941 atcctgaccg ctcgacttca gatacttcac gtagttgtgc aaaccactga tggagagtgc
     9001 atccgggtcc agcgccacca cgcccagttt gtccggtgtg aggttggttt tccaggtgcc
     9061 gctcaccgtc tgcgaaccgg taatctgttt cggattggtc agatcagatt catcaacctg
     9121 cgacagacgc cagactttat gttccgggtc aaacttcgca gtagcggcat agcgtacgga
     9181 ttgcagacga cgattctcgt taaaggcata aatgctgatg ccacctaact cttcgtcacc
     9241 tttaacccgc tcaatgtaga cgaagttgtt gccatctttc gcccataacc cttgctgggt
     9301 agagagcaac gagccgccgt acatcgcctg cgcacggtag ttacgcgcca tctgctcgcc
     9361 ctgcggcgcg acccattcgc caatcgccat cgtcagtaag accagcggaa tggcggtttt
     9421 catcaccgac agcgccacct gcatacgggt aaaaccagaa gcctgcatca ccaccagttc
     9481 gctgcgctgc gccagcatcc caagaccaag caacgcccca agcagagccg ccatcgggaa
     9541 gaagatctgc acatctttcg gcacgctcag caaggtatac attcctgcgc ctaacgcgtc
     9601 gtaactcccc tgcccggctt ttttcagctg atcgacaaac ttgataatgc ccgacagcga
     9661 caccagcatg aacagcgtca tcatgatggt ggtgaaaata gttttaccga tatagcggtc
     9721 aagtacgcca aaaggttgca tcacaccgct cctttacgcg aaaaactggc gcgcaggcgg
     9781 cggaccggca cggtgtccca aaggttgaga acaatcgcca aagccagata aatcaggtta
     9841 acggtccaca tccacagcgt cgggtccagc ttacctttac cgccgttcga tttcagggag
     9901 gtctggatca ggaagaaaag tagatacagc agcatggctg gcagcatcga cagtacgcgt
     9961 ccctgacgtg ggttaaccac gctcagcggt acgaccataa gtgccatcat aaacacggtg
    10021 aataccaacg tgatacgcca gttcagttct gcgcgagcac gatcggtgtc agtgttccac
    10081 aatgtgcgca tgtccatctg gtcggtatcg ttcgggtcga gcgccaccgc ctggtgacca
    10141 atgatcgcct gataatcctg gaaatccgta atgcggaaat cacgtaacag tgcagtgcct
    10201 tcgaagcgcg ttccctggtt gagagtgacg acctgggagc cgtcgcgcag ctgggttaaa
    10261 tgtccggaat cggccaccac cacagaagga cgtgcattac cttttggtcg aatttgtgcg
    10321 aggaacacat ctttgaaatc gctgccgtca acgctttcga tgaacagcac cgagctgcca
    10381 ttagtcgctt gctggaattg cccttgcgcc agcgccgcca tgccagggtt cgctttcgct
    10441 tctgctaaca cttcatcctg atgacgcgat gaccacggtc ccgcccacat cacgttaacc
    10501 gccgcgacga ttgccgtgaa taccgcaagg atcattgccg ctttcaccag aaccgctttg
    10561 ctcaggccgc aggcatgcat taccgtaatt tcactttcgg tatacagttt gcccagcgtc
    10621 atcagcagcc cgaggaacag gcttaatggc aggataagct gcgccatttc cggcacgccc
    10681 aacccgagaa gggagagcac cagattcgcc ggaatatcgc cgtcaaccgc tgcgccgagg
    10741 atcctcacta acttttgaca gaagaagatc aaaagcaaga tgaagagtat cgccagctgg
    10801 cttttgagcg tctcccgcac cagatatctt atgattatca ctttaaatac gcccgtaaaa
    10861 actcgtcttt tgcaggattt tagcttgttt catggcttaa acgtcattta ttctcttgag
    10921 tcgtcgaaat cgtcgctaag ataattatac tcaacggatt cacctctcag attttgttct
    10981 gacgtgccaa tgccgtaata acgttaagat taacacgaag tcatcgcaac agcggacatg
    11041 agttacgaaa gcttgcaatt ctatctgtag ccaccgccgt tgtctttaag attcaggagc
    11101 gtagtgcatg gagtttagtg taaaaagcgg tagcccggag aaacagcgga gtgcctgcat
    11161 cgtcgtgggc gtcttcgaac cacgtcgcct ttctccgatt gcagaacagc tcgataaaat
    11221 cagcgatggg tacatcagcg ccctgctacg tcggggcgaa ctggaaggaa aaccggggca
    11281 gacattgttg ctgcaccatg ttccgaatgt actttccgag cgaattctcc ttattggttg
    11341 cggcaaagaa cgtgagctgg atgagcgtca gtacaagcag gttattcaga aaaccattaa
    11401 tacgctgaat gatactggct caatggaagc ggtctgcttt ctgactgaac tgcacgttaa
    11461 aggccgtaac aactactgga aagtgcgtca ggctgtcgag acggcaaaag agacgctcta
    11521 cagtttcgat cagctgaaaa cgaacaagag cgaaccgcgt cgtccgctgc gtaaaatggt
    11581 gttcaacgtg ccgacccgcc gtgaactgac cagcggtgag cgcgcgatcc agcacggtct
    11641 ggcgattgcc gccgggatta aagcagcaaa ggatcttggc aatatgccgc cgaatatctg
    11701 taacgccgct tacctcgctt cacaagcgcg ccagctggct gacagctaca gcaagaatgt
    11761 tatcacccgc gttatcggcg aacagcagat gaaagagctg gggatgcatt cctatctggc
    11821 ggtcggtcag ggttcgcaaa acgaatcgct gatgtcggtg attgagtaca aaggcaacgc
    11881 gtcggaagat gcacgcccaa tcgtgctggt gggtaaaggt ttaaccttcg actccggcgg
    11941 tatctcgatc aagccttcag aaggcatgga tgagatgaag tacgatatgt gcggtgcggc
    12001 agcggtttac ggcgtgatgc ggatggtcgc ggagctacaa ctgccgatta acgttatcgg
    12061 cgtgttggca ggctgcgaaa acatgcctgg cggacgagcc tatcgtccgg gcgatgtgtt
    12121 aaccaccatg tccggtcaaa ccgttgaagt gctgaatact gacgctgaag gccgcctggt
    12181 actgtgcgac gtgttaactt acgttgagcg ttttgagccg gaagcggtga ttgacgtggc
    12241 gacgctgacc ggtgcctgcg tgatcgcgct gggtcatcat attactggtc tgatggcgaa
    12301 ccataatccg ctggcccatg aactgattgc cgcgtctgaa caatccggtg accgcgcatg
    12361 gcgcttaccg ctgggtgacg agtatcagga acaactggag tccaattttg ccgatatggc
    12421 gaacattggc ggtcgtcctg gtggggcgat taccgcaggt tgcttcctgt cgcgctttac
    12481 ccgtaagtac aactgggcgc acctggatat cgccggtacc gcctggcgtt ctggtaaagc
    12541 aaaaggcgcc accggtcgtc cggtagcgtt gctggcacag ttcctgttaa accgcgctgg
    12601 gtttaacggc gaagagtaat tgcgtcaggc aaggctgtta ttgccggatg cggcgtgaac
    12661 gccttatccg acctacacag cactgaactc gtaggcctga taagacgcgc aagcgtcgca
    12721 tcaggcaatg ctgttattgt cggatgcggc atgaacgcct tatccgacct acacagcacc
    12781 gaactcgtag gcctgataag acgcggcagc gtcgcatcag gcaatgctgt tattgtcgga
    12841 tgcggcgtga gcgccttatc cgacctacac acagcactga actcgtaggc ctgataagac
    12901 acaacagcgt cgcatcaggc gctgcggtgt atacctgatg cgtatttaaa tccaccacaa
    12961 gaagccccat atatgaaaaa cgcgacgttc taccttctgg acaatgacat caccgtcgat
    13021 ggcttaaacg ccgttgagca actggtgtgt gaaattgccg cagaacgttg gcgcagtggt
    13081 aagcgcgtac tcatcgcctg tgaagatgaa aagcaggctt accgactgga tgaagccctg
    13141 tgggcgcgtc cggcagaaag ctttgttccg cataatttag cgggagaagg accgcgcggc
    13201 ggtgcgccgg tggagatcgc ctggccgcaa aagcgcagca gcagcccgcg cgatatattg
    13261 attagtctgc gaacaagctt tgcagatttt gccaccgctt tcacagaagt ggtagacttc
    13321 gttccttatg aagattctct gaaacaactg gcgcgcgaac gctataaagc ctaccgcgtg
    13381 gctggtttca acctgaatac ggcaacctgg aaataatgga aaagacatat aacccacaag
    13441 atatcgaaca gccgctttac gagcactggg aaaagcaggg ctactttaag cctaatggcg
    13501 atgaaagcca ggaaagtttc tgcatcatga tcccgccgcc gaacgtcacc ggcagtttgc
    13561 atatgggtca cgccttccag caaaccatca tggataccat gatccgctat cagcgcatgc
    13621 agggcaaaaa caccctgtgg caggtcggta ctgaccacgc cgggatcgct acccagatgg
    13681 tcgttgagcg caagattgcc gcagaagaag gtaaaacccg tcacgactac ggccgcgaag
    13741 ctttcatcga caaaatctgg gaatggaaag cggaatccgg cggcacgatt acccgtcaga
    13801 tgcgccgtct cggcaactcc gtcgactggg agcgtgaacg cttcacgatg gacgaaggcc
    13861 tgtccaatgc ggtgaaagaa gttttcgttc gtctgtataa agaagacctg atttaccgtg
    13921 gcaaacgcct ggtaaactgg gacccgaaac tgcgcaccgc tatctctgac ctggaagtgg
    13981 aaaaccgcga atcgaaaggt tcgatgtggc acatccgcta tccgctggct gacggtgcga
    14041 aaaccgcaga cggtaaagat tatctggtgg tcgcgactac ccgtccggaa accctgctgg
    14101 gcgatactgg cgtagccgtt aacccggaag atccgcgtta caaagatctg attggcaaat
    14161 atgtcattct accgctggtt aaccgtcgta ttccgatcgt tggcgacgaa cacgccgaca
    14221 tggaaaaagg caccggctgc gtgaaaatca ctccggcgca cgactttaac gactatgaag
    14281 tgggtaaacg acacgcgctg ccgatgatca acatcctgac ctttgacggc gatatccgtg
    14341 aaagcgccca ggtgttcgat accaaaggta acgaatctga cgtttattcc agcgaaatcc
    14401 cggcagagtt ccagaaactc gagcgttttg ctgcacgtaa agcggtcgtt gctgcggttg
    14461 acgcactcgg cctgctggaa gaaattaaac cgcacgacct gaccgttcct tacggcgacc
    14521 gtggcggcgt agttatcgaa ccaatgctga ccgaccagtg gtacgtgcgt gccgatgtac
    14581 tggcgaaacc ggcggttgaa gcggttgaga acggcgacat tcagttcgta ccgaagcagt
    14641 acgaaaacat gtacttctcc tggatgcgcg atattcagga ctggtgtatc tctcgtcagc
    14701 tgtggtgggg tcaccgtatc ccggcatggt atgacgaagc gggtaatgtt tatgttggcc
    14761 gcaacgaaga agaagtgcgc aaggaaaata acctcggtgc caacgttgcc ctgcgtcagg
    14821 acgaagacgt tctcgacacc tggttctctt ctgcactgtg gaccttctct acccttggct
    14881 ggccggaaaa caccgacgcc ctgcgtcagt tccacccaac cagcgtgatg gtatccggct
    14941 tcgacatcat cttcttctgg attgcccgca tgatcatgat gaccatgcac ttcatcaaag
    15001 atgaaaatgg caaaccgcag gtgccgttcc acaccgttta catgaccggc ctgattcgtg
    15061 atgacgaagg ccagaagatg tctaaatcca agggtaacgt tattgatccg ctggatatgg
    15121 ttgacggtat ttcgctgcca gaactgctgg aaaaacgtac cggcaatatg atgcagccgc
    15181 agctggcgga caaaatccgt aagcgcaccg agaaacagtt cccgaacggc attgagccgc
    15241 acggcaccga tgccctgcgc ttcaccctgg cggcgctggc ttctaccggt cgtgacatca
    15301 actgggatat gaagcgcctg gaaggttacc gtaacttctg taacaagctg tggaacgcca
    15361 gccgctttgt gctgatgaac acagaaggtc aggattgcgg cttcaacggt ggtgaaatga
    15421 cgctgtcgct ggcggaccgc tggattctgg cagagtacaa ccagaccatc aaagcgtacc
    15481 gcgaagcgct ggacagcttc cgcttcgata tcgctgcagg cattctgtat gagttcacct
    15541 ggaaccagtt ctgcgactgg tatctggagc tgaccaagcc ggtaatgaac ggtggcaccg
    15601 aagccgaact gcgcggtact cgccatacgc tggtgactgt actggaaggt ctgctgcgcc
    15661 tcgcgcatcc gatcattccg ttcatcaccg aaaccatctg gcagcgtgtg aaagtacttt
    15721 gcggtatcac tgccgacacc atcatgctgc agccgttccc gcagtatgat gcgtctcagg
    15781 ttgatgaagc ggcgctggcc gacaccgagt ggctgaaaca ggcgatcgtt gccgtacgta
    15841 acatccgtgc agaaatgaat atcgcgccgg gcaaaccgct ggagctgctg ctgcgtggtt
    15901 gcagcgcgga tgcagaacgt cgcgtaaatg aaaaccgtgg cttcctgcaa acgctggcgc
    15961 gtctggaaag catcaccgtg ctgcctgccg atgacaaagg tccggtttcc gttacgaaga
    16021 tcatcgacgg cgcagagctg ctgatcccga tggctggcct catcaacaaa gaagatgagc
    16081 tggcgcgtct ggcgaaagaa gtggcgaaga tcgaaggcga aatcagccgt atcgagaaca
    16141 agctggcgaa tgaaggcttt gtcgcccgcg caccggaagc ggtcatcgcg aaagagcgtg
    16201 agaagctgga aggctatgca gaagcgaaag cgaaactgat tgaacagcag gctgttatcg
    16261 ccgcgctgta atcaaaacac agtgatgaaa acgaaggccg gagcatgctc cggccttttt
    16321 tatctcttac agaaatggta aagaaggcat aattccccgt gaaatactgg ctaagaagcc
    16381 tttatctggt tgttcttctt tattgattaa aggaaggtca tccaaatcgc ccagcaaata
    16441 cgtatttttt gcctgccagt caatcatcca tattttcagc agtggataag ccagaccgcc
    16501 cgtaatcccg gatatcacca ccaacgcaca catgcgataa agcataccgt ggtatgttaa
    16561 agttgagcga aaacgaatac gcccatcatt cagtgacagg ttgctcataa aatgatttcg
    16621 caacgacacc gttaaataac tggtgctaac agcaatccca aaataataga ttaactgcgc
    16681 gattatcatt ttacgttgca tttccataaa ttgctgtaaa ttctcaatat catcatttgc
    16741 atacacagaa ctatcatacg catttaatat ttgatcgaag ataatataac cagcaacagc
    16801 gagaaaaggt aataaagcta aaaatgcgag gatggcatat tttatacaat atgcagtatc
    16861 taatttcacc ttgaaacgat gtataccgaa actggtattg ctccagagaa aactcattac
    16921 caggctatat aaagtaccat taaaaatacc aatggaaaca ataccaacta ttgccatcag
    16981 aaccacagat ataataacac tacttgaact attggcatgt agcatctttg tcgagataaa
    17041 gaaaacagtc cccatcccaa tggccattaa aatcggcaag aaaaaggtta cccaccagaa
    17101 ccttttcata gagcaattaa aactaaatct tacgccgttg agactggtca tcaaggcctg
    17161 ataacgcagt ccttttgctg ccataaatat aagcaaaacc aacagtgaca aagtcaaaac
    17221 acagccaaca agcggcatat ctgctgacac tgtcattaag attgcgaaat agaaaaaaac
    17281 aaaaacaaga caactaaaaa aaacattccc accagtaatt ccataagaaa atcgttgtcc
    17341 gttaacttcc atattagcat agagataacg cttacatttc attaatgccc atggtaaata
    17401 gatacctagc gttataatcg ttaacaacac attcaccaca caaataagaa aatatctctc
    17461 acctgtacca tgaaaaacaa acgaatggga cggaacatcc atttcattaa taacttgagc
    17521 cataaaaatt ccctgaatta aaaaatccat tgatataatt tatagataca gacagtaaaa
    17581 tgttctgcca cgttacaata ctcttatcag taataggctg gcaaaaactt tattgcttta
    17641 taagatgctt gcgcacgtaa gaagagagtg gtattaaaca aatgtataat tactttattt
    17701 ttattgggtt aaactatgaa taacattgcg ccgcaatcac ctgtaatgcg tcgcctgacg
    17761 ctgcaggata atcctgttat tgcccgcgtc attcgtcagg tatccgccga atacggtctt
    17821 accgctgata aaggctacac cgtcgccgat ccgaatcttg acgaactgta tcaagtgtat
    17881 agtcagccgg gtcatgcata ttgggtggtt gaatacgagg gtgaagtggt cggcggcggt
    17941 gggattgcgc cattagcagg tagtgaatcg gacatttgcg aactgcaaaa gatgtatttt
    18001 cttccggcta ttcgcggtaa agggctggcg aaaaaactgg ccttaatggc gatggagcag
    18061 gcacgagaaa tgggcttcaa acgctgctat ctggaaacga ccgctttttt aaaggaagcc
    18121 attgcgcttt atgagcattt gggctttgag catatcgact atgcgcttgg ctgcacgggc
    18181 catgtcgatt gtgaagtgcg gatgctacgc gaactctaaa aaaatgcccg ctagtgtgtg
    18241 ctatcgggca ttttaatcaa ttaattagtg gcgaaccccg tcatcgtctt catcgacaaa
    18301 atcttcatcg tcgccatctt cgccgttagg atcttcaaag taagtgcccc aaccgtcgta
    18361 ctcaacgtca aatttctccg ccagcgtcat cagttgttca acctgggcat cgatcagatc
    18421 ggcattcaac gcgcactcgc tgaggatgtc gcagcaaatc acgatatcac catcttcaac
    18481 ttccagctct tctggatcgg tcacttcgta accgagttta aacgcttcaa ctgctgcttt
    18541 ttccagggtt tccagatcgt ctgcggaaag atggtgttcg atggtgtaca gcgcgtccgg
    18601 atcgctgcca tcttccagta attcttcaat aatcaaacgt gtttcttcac gctgttcttc
    18661 cagttgttcc gggtttgcca tggctcattc ctcttaatgt cctgccgata ctcatattgt
    18721 cacacaccac tggcattgcc tccacctttg tgacaaagat ttatgcttta gacttgcaaa
    18781 tgaataatca tccatataaa ttgaatttta attcattgag gcgttagcca caggagggat
    18841 ctatgtccgg gttttatcat aagcatttcc tgaaattact cgatttcacg ccagctgaac
    18901 tcaacagcct gctgcagtta gccgcgaagc tgaaagccga taagaaaagc ggcaaagaag
    18961 aagccaaact cactggtaaa aacatcgcgc tcatcttcga aaaagactcg acccgtaccc
    19021 gatgctcttt cgaagttgcc gcatatgacc agggtgctcg cgttacttat ctcggcccaa
    19081 gcggcagtca gattggtcat aaagagtcga ttaaagacac tgcccgcgtg ctgggtcgca
    19141 tgtatgacgg tattcagtat cgcggctatg gtcaggagat tgtcgaaaca ctggcggaat
    19201 acgctggcgt gccggtatgg aacggcctga ccaatgagtt tcaccccacg cagttgctgg
    19261 cggatctcct caccatgcag gagcatttac ccggtaaagc attcaacgaa atgacgctgg
    19321 tctatgcagg tgacgcacgt aacaacatgg gcaattcgat gctcgaagct gcggcgctta
    19381 ccggtctgga tttgcgtttg gtcgcgccac aggcatgctg gccggaagct gcgctggtta
    19441 cggaatgccg cgctctggca cagcaaaatg gcgggaatat tacgctgacg gaagatgtag
    19501 ctaaaggcgt tgaaggtgct gactttatct ataccgatgt atgggtgtcg atgggtgaag
    19561 caaaagagaa atgggcagaa cggattgcat tactgcgtga ttatcaggtg aacagcaaga
    19621 tgatgcagtt gaccggtaac ccggaggtca aattcctcca ctgcctgccc gcgtttcatg
    19681 acgaccagac gacgcttggt aagaaaatgg cggaagagtt tggcctacat ggcggaatgg
    19741 aagtgactga tgaggtcttc gaatctgccg ccagcattgt ttttgatcag gcggaaaacc
    19801 gtatgcatac catcaaagca gtgatggtcg cgacgctcag taactaattg atatcgctat
    19861 aaagatgccg ggaaatacaa aactatcccg gcatcacatt attttatgca gtgataggtc
    19921 tattcttcca cccaattttt tctattgcag aacgtaaatc ttgcggtgga tttaattttt
    19981 ctaactgttt ttcattaata taatcatata tattgatttt cccatcccgc agggtttcca
    20041 tcattcttgt tctaattttt aattcattac ccatcttttc ttcaaaaatc ttaacgacat
    20101 aacccggata tttttttaca atttcatttg t
//
LOCUS       DAZFKF010000092        20111 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFKF010000092 DAZFKF010000000
VERSION     DAZFKF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.092"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..575)
                     /locus_tag="ACHKA1_003866"
     CDS             complement(<1..575)
                     /locus_tag="ACHKA1_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HFO5681454.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHSIKCQNCDSEIWFSGIDIDTQILFQTAEVDAFSSSNGVSIINGVILNDVSWDVVIE
                     STEVNDLNLFVWRFFERYVVSSFFIKGKEFCCESDKYRFNKIIPYLLGYSHEYSAQRM
                     DVSIDQSKKIFMNFKKHYGFSDRDEWLRYAI"
     gene            complement(769..1152)
                     /gene="traM"
                     /locus_tag="ACHKA1_003867"
     CDS             complement(769..1152)
                     /gene="traM"
                     /locus_tag="ACHKA1_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO5681455.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQIEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDEE"
     gene            1473..2075
                     /locus_tag="ACHKA1_003868"
     CDS             1473..2075
                     /locus_tag="ACHKA1_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO5681456.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICHATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAVGAYNAGFRKSERQNQ
                     RRLAYASEVYRIYTGIKSSKGIRLPATKKSLPEINSVQNN"
     gene            complement(2372..3193)
                     /locus_tag="ACHKA1_003869"
     CDS             complement(2372..3193)
                     /locus_tag="ACHKA1_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5681457.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(3313..3600)
                     /locus_tag="ACHKA1_003870"
     CDS             complement(3313..3600)
                     /locus_tag="ACHKA1_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681458.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSQGVDKQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            complement(3625..3831)
                     /locus_tag="ACHKA1_003871"
     CDS             complement(3625..3831)
                     /locus_tag="ACHKA1_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5681459.1"
                     /translation="MHIPQNFLPRSRQKLLRSAPGQSFPAVAVMPHAAKVLKQVMVNT
                     GGNKRQVPAKRQNRGSRNSTDDGG"
     gene            3901..4074
                     /locus_tag="ACHKA1_003872"
                     /pseudo
     CDS             3901..4074
                     /locus_tag="ACHKA1_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4072..4302)
                     /locus_tag="ACHKA1_003873"
     CDS             complement(4072..4302)
                     /locus_tag="ACHKA1_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681460.1"
                     /translation="MACQKSHHLPCDYSLFLPALILTTAVLSCLPGAPSAPGCLAASG
                     RQARLRQAAAGAPASLRRTRWRGFGLSLRSDA"
     gene            complement(4522..4647)
                     /locus_tag="ACHKA1_003874"
     CDS             complement(4522..4647)
                     /locus_tag="ACHKA1_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO5681461.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
     gene            complement(4589..4738)
                     /gene="mok"
                     /locus_tag="ACHKA1_003875"
                     /pseudo
     CDS             complement(4589..4738)
                     /gene="mok"
                     /locus_tag="ACHKA1_003875"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            4760..4948
                     /locus_tag="ACHKA1_003876"
     CDS             4760..4948
                     /locus_tag="ACHKA1_003876"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681462.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQTVHGKSV"
     gene            complement(4917..5679)
                     /locus_tag="ACHKA1_003877"
                     /pseudo
     CDS             complement(4917..5679)
                     /locus_tag="ACHKA1_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            complement(5676..6110)
                     /gene="psiB"
                     /locus_tag="ACHKA1_003878"
     CDS             complement(5676..6110)
                     /gene="psiB"
                     /locus_tag="ACHKA1_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO5681463.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQQRFAPEAISHSLALAASLDEQGYSVNDIIHILMAEGGQA"
     gene            complement(6179..8143)
                     /locus_tag="ACHKA1_003879"
     CDS             complement(6179..8143)
                     /locus_tag="ACHKA1_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO5681464.1"
                     /translation="MSVTESKVKTERKSSRKPAKTQETVLSALLAETAEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNTKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWGWCAGRMEAVGECREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDAGRTEQMQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSNPARIRLECEHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIYGVQTREYGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIDALNDAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPDWMCAPRPQAETETTEYRDNQADAA"
     gene            complement(8208..8441)
                     /locus_tag="ACHKA1_003880"
     CDS             complement(8208..8441)
                     /locus_tag="ACHKA1_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO5681465.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAVQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            complement(8503..9042)
                     /locus_tag="ACHKA1_003881"
     CDS             complement(8503..9042)
                     /locus_tag="ACHKA1_003881"
                     /inference="COORDINATES: protein motif:HMM:NF010292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5681466.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSGQPQPEPQAEAGTKKGGAKTKGRGRKAAQPE
                     PQPQPPEGDDYGFSDDIPF"
     gene            complement(9068..9274)
                     /locus_tag="ACHKA1_003882"
     CDS             complement(9068..9274)
                     /locus_tag="ACHKA1_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5681467.1"
                     /translation="MHIPQNFLPRSRQKLLRSAPGQSFPAVAVMPLAAKVLKQVMVNT
                     GGNKEQVPAKRQNRGSRNSTDDGG"
     gene            complement(9515..9748)
                     /locus_tag="ACHKA1_003883"
     CDS             complement(9515..9748)
                     /locus_tag="ACHKA1_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681468.1"
                     /translation="MSVTDVVVPATATVLLFPAALILTTAVLSRLPGAPSAPGCLAAS
                     GRQARLRQAAAGAPASLRRTRWRGFGLSLRSDA"
     gene            complement(9685..9903)
                     /locus_tag="ACHKA1_003884"
     CDS             complement(9685..9903)
                     /locus_tag="ACHKA1_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681469.1"
                     /translation="MKTPGRREDKCPSARAGKRPRAGGAERGRAHAPPLSLRGRSDGG
                     FCFFMPVICLLLMLLFLLLLLFFFFLLL"
     gene            complement(9900..10463)
                     /locus_tag="ACHKA1_003885"
     CDS             complement(9900..10463)
                     /locus_tag="ACHKA1_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO5681470.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRDNFNGVRVQCG
                     DFMEWQPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(10511..11872)
                     /locus_tag="ACHKA1_003886"
     CDS             complement(10511..11872)
                     /locus_tag="ACHKA1_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO5681471.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDQETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLQAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAIASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(11924..12154)
                     /locus_tag="ACHKA1_003887"
     CDS             complement(11924..12154)
                     /locus_tag="ACHKA1_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681472.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(13192..13383)
                     /locus_tag="ACHKA1_003888"
     CDS             complement(13192..13383)
                     /locus_tag="ACHKA1_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681473.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(13380..13802)
                     /locus_tag="ACHKA1_003889"
     CDS             complement(13380..13802)
                     /locus_tag="ACHKA1_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO5681474.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGENMS"
     gene            complement(13849..14274)
                     /locus_tag="ACHKA1_003890"
     CDS             complement(13849..14274)
                     /locus_tag="ACHKA1_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5681475.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADACGIILTSLVINRQLW
                     LYHDSGDAELTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(14687..15457)
                     /locus_tag="ACHKA1_003891"
     CDS             complement(14687..15457)
                     /locus_tag="ACHKA1_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681476.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIYVL
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLAEDEIRAVLDAVRDAVCSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILASGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRG"
     gene            complement(15502..16020)
                     /locus_tag="ACHKA1_003892"
     CDS             complement(15502..16020)
                     /locus_tag="ACHKA1_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO5681477.1"
                     /translation="MAPIACLWRRIPKFWNWIPASKIEKETRMYGTCETLCRELAAQY
                     PGDTPLMLVVWSPEEIQALADGMDISLSDHEIRTVLARLEDIPEDQRIESGISSAAAM
                     EIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDHGLAVPECVTRRQAVVNQA
                     RTLLKNNTREND"
     gene            complement(15950..16171)
                     /locus_tag="ACHKA1_003893"
     CDS             complement(15950..16171)
                     /locus_tag="ACHKA1_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681478.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            complement(16172..16855)
                     /locus_tag="ACHKA1_003894"
     CDS             complement(16172..16855)
                     /locus_tag="ACHKA1_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO5681479.1"
                     /translation="MSRFIQGDCVRVMATFPGNAVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(16932..17225)
                     /locus_tag="ACHKA1_003895"
     CDS             complement(16932..17225)
                     /locus_tag="ACHKA1_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681480.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            17373..18335
                     /gene="parM"
                     /locus_tag="ACHKA1_003896"
                     /gene_synonym="stbA"
     CDS             17373..18335
                     /gene="parM"
                     /locus_tag="ACHKA1_003896"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO5681481.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEALQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            18338..18688
                     /locus_tag="ACHKA1_003897"
     CDS             18338..18688
                     /locus_tag="ACHKA1_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO5681482.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRAPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSTITQPSSQQEQR
                     SDEETKKNAMKLIN"
     gene            complement(18839..19351)
                     /locus_tag="ACHKA1_003898"
     CDS             complement(18839..19351)
                     /locus_tag="ACHKA1_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681483.1"
                     /translation="MNISVEDLFPESFQRIKEEEEANRPDPNWFAHLPPEKINAYMSK
                     YPFKSFDDIPLDNSGLQYPSLQELEFYFPPAALAANCHQLPERMRQKPVVTMINGLTL
                     LRSLGVQAFNIDPRRWHKIKTYLSQGTIEYPQMSEDGKVIIDGRHRILLIMQIYKVTD
                     VPVFFLKGIS"
     gene            19792..>20111
                     /locus_tag="ACHKA1_003899"
     CDS             19792..>20111
                     /locus_tag="ACHKA1_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase
                     domain-containing protein"
                     /protein_id="HFO5681484.1"
                     /translation="MSDNEGSVPTEGIDYGDTMVVWPSTGRIPGGDVKPGGSSGLAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAA
                     RTVL"
BASE COUNT         4786 a         5605 c         5036 g         4684 t
ORIGIN      
        1 attatagcat atcgcaacca ctcatccctg tctgagaaac catagtgttt tttaaaattc
       61 ataaatattt ttttagactg gtcaatagag acatccattc tctgagcaga atactcatga
      121 ctataaccca aaagatacgg tattatctta ttaaagcgat atttgtcaga ttcacaacaa
      181 aactccttgc cctttataaa aaagctacta actacgtatc tttcgaaaaa tctccagaca
      241 aacaaattca agtcattcac ttcggtactt tcaatcacca catcccatga tacatcattc
      301 aatattacac cattaataat gctcactcca tttgaggacg aaaaagcatc aacttcagca
      361 gtctgaaata aaatttgcgt atcaatatca ataccagaaa accatatttc agaatcacaa
      421 ttctgacact ttattgagtg taaaaaaaca gagtttgagt ctatgaattt tcctgactct
      481 gtgcggacac agactggata ttttgcggat aaaataattt ccggcaggcc tgattgacgt
      541 atgagtgctc tttctctagt atccatcgca cacataggaa cctcctcaaa ggattctatg
      601 gacagtcgat gcagggagct agtccctccc tgcatcggcg attttcaagt tcaaatttta
      661 acgtggcatt aaccacataa ttaatatact cgttatgtgg ttaattgtca tcaaatagaa
      721 ctagatcaat attctgttat ttgaataaaa tcaactttta attctttttt attcctcatc
      781 attttttgga aaaaatcgtt ccatctcaga tgacaccttc tcccggatat cttcaaccat
      841 attggcatat tcaaactttg gattcccgga aacatgagga ctgagagact caatacccaa
      901 aattttcgct actgatgatt gtgtttttac aacgcattca agaagtaatt tattaaactc
      961 aatctgatta aacgcggact ctttacgctc catctgagct tcatatacac gaagccccag
     1021 ttcaagaagc attgaagctg ttgctgaaaa actgacatct ttttctcttg ccccttcctg
     1081 tcgacgcttc tcaataatcg catttatttt ttcataagca tcattgctga tatacaggtt
     1141 caccttagcc attagaaatt accttttttg aacataaaat aaagttaatt tagaaacacc
     1201 agagttcata atgcaaacag ggacgcaccg ctagcagcgc ccctggcggt atcctataaa
     1261 aaaatacacc gcgccgctag cagcacccct aatataaaat aatgtttttt ataaaaatag
     1321 tcagtaccac ccctaaaaag cggtgtctgc gcgttgctgt agcggcgtta acgacgctgt
     1381 tttaaataaa tcagatttaa acaatataaa tccgcaaata caactcaatg atattaaacg
     1441 aaaatcagca aaaacttgtt tttgcgtagt gtgtggtgct tttggtggtg agaaccacca
     1501 acctgttgag cctttttgtg gagtgggtta aattatttac ggataaagtc accagaggtg
     1561 gaaaaatgaa aaaatggatg ttagccatct gcctgatgtt tataaatgag atctgccatg
     1621 ccactgattg ctttgatctt gcaggccggg attacaaaat agatcctgat ttactgagag
     1681 caatatcatg gaaagaatcc cgttaccggg ttaatgccat cggtattaat ccggtaacgg
     1741 gatatggcag cggactgatg caggtagatt cccagcattt taacgaactg gcccgctacg
     1801 gaattaagcc agaacatctg acaacagatc cctgcatgaa catttatacc ggtgcttatt
     1861 atctggcaat agcatttaaa aaatggggcg tcacctggga ggccgttggt gcatacaatg
     1921 caggattcag gaaatccgaa cgccagaacc agagacgtct tgcctacgca tcagaggttt
     1981 accggattta taccgggata aaaagcagta aaggtatccg gcttccggcc acgaagaaat
     2041 cacttccgga aattaacagc gtgcagaaca attaatctta cagacattgt cagataccgg
     2101 ttatgccgca aaagcggcat ggaaggccac acacaaaaat atcatatgag tgacaatact
     2161 gactggccga agccccgcga agcgggaacc catcttatat atacccggta actgcgcagc
     2221 ctcctgccag accatttttc gcgggcacaa tatttacaga caaccggaaa gccggtcgat
     2281 gaaattgagg tatatcagac gacacagcaa tgccgaaaaa tgataatata agtaaagccc
     2341 cgaaaatttt ttcggggctt tacttatatt atcatgactt ccactttctg aactgttcgg
     2401 caatcaccca taacgccttg ttgattctta tatcaccatc aatacctgta atggctcttg
     2461 tcctggtatt tttcccggag gcacttcgcc ccgataatcc accttttatc atattctcct
     2521 gaacccgctg ccaggttgtc cagagatcgt tctgtttgtc ttcccgacga cggggagtaa
     2581 ttatctgttc aggtgtcact ggcgttttat tttcatcttc atacctgacc atcagtgcag
     2641 ctctgccaaa taaatgttgc tcgtcactgt taagatgaat ttctttcatc gcctccatat
     2701 tatcagtgac cttatcaaag acaccgagca cttcatacgc tccctcgata acctgcccga
     2761 caatatcacc tttatgtgga acgcggattt cgccaaaatt atttccgcac accaggccat
     2821 ttgtgcagac aaaacgaaaa attccgggga tcatctgata actggatgaa ccatcatgtg
     2881 aattaagcag gataatttca ggaacttcct gtccgttaat atgcccttcc ctgcgaagac
     2941 gtaacatatg tttactgtat tcgcgacgtc ccaaatcacg aacccgactc tgacaggcaa
     3001 agaatggctg gaaaccttca tcacgtaact tattgatgat attgattgtt ggaatatacg
     3061 tataacgttc actccgggac tcatgtttat caccggaaaa taccgaaggc acgaactgca
     3121 ttaattcatc atccgttaaa ggacgttcac ggcggatgga attataccga ccaaaacggg
     3181 aagctaatcg cataacagct actcctttat gaaagaataa atttaataaa aaatttttcc
     3241 gcttaatatc atcccccccc gatctccatt aacaagattg caacaatcag gagggatatt
     3301 catcacatcc ggtcagcaga tcgcccggat atcgaggccg gcacgcttaa tctgacgcag
     3361 gaactgtttg aagtgatcgg cttccgcaat gttgatatgg cggaactgct tatccacacc
     3421 ctgactgagc gcgtagctat aactgaaggc catcttttcc gggtggcttt tgcgatattt
     3481 tttcagctca gtcaggctgc tgaattccag gacgtcccac tcttccccgg tgtttttttt
     3541 cttcccggca acaagaagcg tgtacgacgc ggtgttaacg tggatatttc gggtactcat
     3601 cctgctttct cctctctctg gttttcagcc cccatcatct gttgagtttc gggagccgcg
     3661 gttttgacgt tttgcgggga cctgcctttt gttccctccg gtattcacca tcacctgttt
     3721 gagaaccttc gccgcatggg gcatcactgc cacggccgga aacgactgtc cgggggcaga
     3781 ccgcaaaagt ttttgcctgc ttctgggcaa aaagttttgc gggatgtgca tttcacccct
     3841 ggactggcgg agacggttgt ggctgtctat gcccccggcg acaggggctt aaacaggtga
     3901 atggcaccgg acacaacaac gggcagggac ccgccgcaac gacgtgaagc gcggtttgcg
     3961 aaacggcgtt gcgccttaca tcccggcggc cttttgccgg gatgccagga gcgcaggtca
     4021 cggcgcgaca gaggcggcgc gaccggaacg gtcgctgcaa gcccggggtt ttcacgcatc
     4081 ggaccgtagc gacagcccga aaccgcgcca gcgggttcgg cggagactgg caggtgcgcc
     4141 agcagctgcc tgtcggagtc gggcctgacg accggatgcc gccaggcagc cgggagccga
     4201 aggtgcgccc ggcagacagg acaacactgc agtagtcaga atcagagcag gaagaaaaag
     4261 agaataatca cagggaaggt gatgtgattt ctgacaggcc ataaccagag aaggcggggg
     4321 aaaagaacgg gcgccgcagg cgcgcgttca tccccgttcc tgaagggaaa cgaccttcct
     4381 gaagtcaggt tgttcatgcc cgtacaacat cagcaaggag aaaggggcta ccggcgaacc
     4441 agcagcccct ttataaaggc gcttcagtag tcagaccagc atcagtcctg aaaaggcggg
     4501 cctgcgcccg cctccaggtt gctacttacc ggattcgtaa gccatgaaag ccgccacctc
     4561 cctgtgtccg tctctgtaac gaatctcgca cagcgatttt cgtgtcagat aagtgaatat
     4621 caacagtgtg agacacacga tcaacacaca ccagacaagg gaacttcgtg gtagtttcat
     4681 ggccttcttc tccttgcgca aagcgcggta agaggctatc ctgatgtgga ctagacatag
     4741 ggatgcctcg tggtggttaa tgaaaattaa cttactacgg ggctatcttc tttctgccac
     4801 acaacacggc aacaaaccac cttcacgtca tgaggcagaa agcctcaagc gccgggcaca
     4861 tcatagccca tatacctgca cgctgaccac actcactttc cctgaaaata atccgctcat
     4921 tcagaccgtt cacgggaaat ccgtgtgatt gttgccgcat cacgctgcct cccggagttt
     4981 gttcggcacc atccaccgct ccatctcacg gacagcgtga ccggcctctc ttgctgcaag
     5041 actggcttcc gcaataacct gccagaacgg catatcctgc cactgccagc gttccattcc
     5101 ggccagtgac ggaaaacgct cgccccactg ccagaacagg gtttccaggt catgctctgc
     5161 cacccggtca ctccagcgtg catgccagct tccgactgtt tccggcgaat ggaatgccag
     5221 ttcgatttca gcctgagcga ccagcgcctg gtacgcacgg gctttctgct ctgcctccct
     5281 cgcctgctgg cggctgtact tctgcatgac cagcgcggat ttatggcgac tgcgttccct
     5341 gacacgaaac cgcaccgacg ggaataaccg ctctgctgca tcgccgggaa ggggaaggag
     5401 gcagacctcg ccctgtgttt caatcagcac atcgatggca tacagccact gaagacggtt
     5461 gccgtgcagt tcttctgcgg tcagggacgg catgaaaaat cgcaggtcag acagcgatat
     5521 cctgcggctt ccgttcagac agcggaaaaa cgcccccgca tacggatatt cagccagcgt
     5581 gttaccctga tggcggcgtt tttcggtctc cgccaccgcc cgccatgcgg cctgttgctc
     5641 tgtgctgaga ggaaccagtg cctgtgaacg tgcgctcatg cctgacctcc ttccgccatc
     5701 aggatatgga tgatgtcatt aacgctgtat ccctgttcat ccagtgacgc tgccagtgcc
     5761 agtgaatggc tgatggcttc cggtgcaaac cgctgctgta cctgaaccac cgcaaacggc
     5821 tcaccaccgg cattgaccag aaccagcacc cagacctgag agacatcacc cggtgaacat
     5881 aacgccaggt gaaaacgttc gtgggcaggc gtgaaacgga cctggacggg gaaaaagccg
     5941 ccaaactcgc tgccgtactc gccgttcatc gtccagttat ccggcgcatc cagctcccgc
     6001 atcacggcgt gagtcagctc acggcgttca tcacttccgg cagcccggat atcttcatat
     6061 tcctgtgcgt tcatggactg cagggcattc agggtcagtt cagttttcat cacggtatct
     6121 cccttgctgc cgccccgtct ccggcggggc ggcgtgataa atggtttata actgacggtc
     6181 aggcggcatc cgcctggttg tcacggtatt ccgtggtttc cgtctctgcc tgtggacgtg
     6241 gcgcacacat ccagtcaggc acccagcggt tgtctttcat gtggtgctct gcatgttcag
     6301 ccgcatcgcc tttcttcatc ttctccgcgt cccgtgcggc accggacagc ccggcgtcat
     6361 tcagggcgtc gataatctgc ggctttttca ggtgtccgaa gaagtttgct ttcgtcggct
     6421 gccaccagtc gcgcatgtgg aatccgatag ccgtttccag cgagtccagc ggactgcgtg
     6481 acgtgtgacc atactcgcgg gtctgaacgc cgtagatgct gcacgcggtg cagaaactga
     6541 gcagggataa cagtacctcc tgactgagtg acaggaacgt cgtcatatcc cgtgaccatc
     6601 cctccggcaa cagggcggca agacgggctt tttctgccat cagcgccatg aatgcggcac
     6661 cttccttccc cgatggcgca tcgctggtca gcgagtaatg ctcacattcc aggcggattc
     6721 tggcgggatt gctgtacgct ccgctgccaa acacattcag acagagtgtc catgccagca
     6781 gtgtcaggga tttgtccggc tgctgcatca gtgccgcctg gactgccagc gtgcgctctg
     6841 aggacatttt cgtcagcagc ggcagactga tttcctccac ggaggctttc tcctgcatct
     6901 gttccgtgcg gccagcgtcg tcagccgcgt catcctcact gcgtaactgc acaccacgct
     6961 ggacacatac gtttccataa cgccaggaca ccaccacacc acttccggca cgcatctccg
     7021 gcgtccacgc tctgaccttc gccatgcagt caatcagctt catttctgct tccagcaggt
     7081 cggattcctc acactggttt tccagcgcat cgtaacgtgc catcagttcg ttcaggcgtt
     7141 cttcttctgc ctccgtcagc accgcttccg gctccggcag actgcggtat gttccggcat
     7201 cctcacggca ctcaccaacg gcctccatgc gtccggcaca ccatccccag ccctcggctt
     7261 cccgaaggtg ttcagcaaca gcctggagtt tttccagcag ggcagcatcg agcgcaacac
     7321 agtccacata gccaccctcg tcgtcgctga acaaatcggt gcgcagttca tctggcgaga
     7381 aggcatcagc ccccacaaag cggaatttag tgttccccgc caccgccact tcactttcgg
     7441 tcaccagacg acgaatggtc tgtacttccg gttttccgcc ccatcccgac tgacaggcag
     7501 cttcaaacac ctgcacctga cgcgcggtgt cgttctccag cgccagcgcc tgacagtgct
     7561 ctgtggtgat gcggtcttct gccagcgcat cgaggatgac aggcgcaagg tctgccagtt
     7621 tcagcattcg ctgaacgtgg cggggtgaat agcccagcaa atcaccgatt tgtgcaggcg
     7681 ttttgccttc ctgcgccatt gcacggaatc cggcaatctg ttcggcaggg tgcatatccc
     7741 gacgatgacc gttttcagtc attgatgcgg cagtcgccag ttcctgcgga ataaccttca
     7801 cgcggacagg ccagtcagcc ggaaggatgt tacgctctgc cagcatgttg agtgctgcca
     7861 gtcggcgacc gcctgcggcg acaccgtaac ggtcacctgg cagggcatga acaaccagat
     7921 tctgcagcag gccgacgccc ttaatggact ctgccagttc gctgacggac tccgcagaat
     7981 acggcaccgt gcgcacattc agcggtgact taatcagcga ggccagcggc acgctcactt
     8041 cagccgtctc cgccagcagg gccgacagga ctgtttcctg cgtttttgca ggtttacggc
     8101 tggatttacg ctccgttttt accttagact ctgtaactga catatatttc tcctttgacc
     8161 ggggtggttg tatgtctgcc ggacgtgatc cccgtccggc acctctctta cttaagaatc
     8221 ccgaaatcac ggatgacatg gttcatccag tacccggcac cgtcctcaaa attccaggta
     8281 cgccagacca ttgcaccgtt attacggaca accaggcgaa actgttcgcc gtgatcatcc
     8341 tcgataaaga cgttccggta ctgtacggca acggcttcgg cctgttcgcg ggtaaaggag
     8401 ccgtcaggca gcccctgaag tattctgaaa tattcgctca tcctcatctc tccggtgatg
     8461 ccccgtacag gacggggcgg ggccgttgtc acagtcagtc gttcagaacg ggatatcgtc
     8521 tgaaaacccg taatcgtcac cctccggcgg ttgcggctga ggctccggct gcgcggcctt
     8581 acgtccacgg cctttcgttt ttgcgccacc ttttttcgta ccggcctccg cctgtggttc
     8641 cggctgaggc tgaccgctga actgttgccc ctcttccggc tgagtctgag cacctgcggc
     8701 acgtcccagc atctgcatgg tgcccgtggt cttaacaaga atttcggtga cgtaacgggt
     8761 gataccgtta tcttcccagc tacgggttcg gagttgcccc tcgatgtaga cctgcgcgcc
     8821 cttgcgcaga tattcacctg ccacttccgc gagcttgccg aacagcacca cgcgatgcca
     8881 ttctgtctgc tcccgcatct cccccgtctg tttgtcacgc cagctttctg acgtggccac
     8941 ctgcaggttt gccactgcgc ccccgttggg gatgtaacgg acttccggat cttttcccag
     9001 acgaccgaca agaatgacct tgttaattcc acgaactgcc atgatgtgtc tccttctgtt
     9061 gatgttttca gcccccatca tctgttgagt ttcgggagcc gcggttttga cgttttgcgg
     9121 ggacctgctc tttgttccct ccggtattca ccatcacctg tttgagaacc ttcgccgcaa
     9181 ggggcatcac tgccacggcc ggaaacgact gtccgggggc agaccgcaaa agtttttgcc
     9241 tgcttctggg caaaaagttt tgcgggatgt gcatttcacc cctggactgg cggagacggt
     9301 tgtggctgtc tatgcccccg gcgacagggg cttaaacagg tgaatggcac cggacacaac
     9361 aacgggcagg gacccgccgc aacgacgtga agcgcggttt gcgaaacggc gttgcgcctt
     9421 acatcccggc ggccttttgc cgggatgcca ggagcgcagg ccacggcgcg acagaggcgg
     9481 cgcgaccgga acggtcgctg caagcccggg gttttcacgc atcggaccgt agcgacagcc
     9541 cgaaaccgcg ccagcgggtt cggcggagac tggcaggtgc gccagcagct gcctgtcgga
     9601 gtcgggcctg acgaccggat gccgccaggc agccgggagc cgaaggtgcg cccggcagac
     9661 gggacaacac tgcagtagtc agaatcagag cagcaggaaa aagaagaaca gtagcagtag
     9721 caggaacaac aacatcagta acagacaaat gacaggcatg aagaaacaga agcccccatc
     9781 agaacgtccg cgtaatgaaa ggggcggcgc gtgagcgcgg cccctttcag cgccaccggc
     9841 gcgcggtctt ttcccggcgc gggcagacgg gcatttatcc tcccgcctgc ccggcgtctt
     9901 caggcacgca gacgaataat catcgtcggg acatcggtat aagcaaacgt accgcgtggc
     9961 agctcctcgc ggacgtcaga aaacggtaac agcttctcct gctggcgcgg cccgttcaga
    10021 cagacggcaa ccagcacgcc acccggacgc aacagggaaa aggcccgcag gatatgccgg
    10081 atatcctgcc cgtggctgaa cggcggattc atgataaccc ggctgtaata ctgcaccggc
    10141 tgccattcca tgaaatcgcc acactgaacc ctgacaccgt taaaattatc ccgcagatac
    10201 cggaccagcc cgctgttgat ttccaccgca tcacacatgg cccccggcgc ggtatcccga
    10261 atcgcccgca gtatggcccc ggtgccggca gagggttcca gaatgtgatc acggttgctg
    10321 atctccgcca gcgtcaccag gcgatcacag accggggcag gagtcacaaa aagctgagat
    10381 tcagcccgga tttcagcaac cggcgcggcg ttatccagcc ttgcgcgcat cagccggata
    10441 cgttctctta actgactgtg catatcatta tctccgtcat cccccgggga ttttcccggg
    10501 gggatatacc ttatttctgt ggaatttccg tgattttcat gtcggtgata tacacattca
    10561 ccagacggaa attattgtcc attgtgcggc ggtagcggta tgccccgtgg tcttctgctt
    10621 ctgccacact gcgcacggcc ttacagtccc ggggcagggc ggcccactgt gcctttgtca
    10681 tttcccggaa accttccccc ggatagttga ctaccggcgg acgcttcgcg gcctggctgg
    10741 cgatggcagc ctcttctgcc gatggtgctt tgtagtccgt gatgcgatcg ggcgtcactt
    10801 tcatcgtgcc gctgtacccg agaaaactgt aattcggcgt tgtgactgaa ctcaccgccc
    10861 cgttgctttt gttcacgcgg atgatggtca gccactcgcc ccggctgaaa acctgtccgc
    10921 ccggctcaaa atcctgtgtc cgggtaacca cgccgccgct ttcgtccagc atggcacgct
    10981 catagatcag gcggttctga tagtggttaa cccagcgttg ctgatgctga atctgccgtt
    11041 catgacagcg gatcgcaatt tcgcgggcct gctccgtggt gatgatgtca tcatccaggg
    11101 ccgaccataa cgaccggctc ccttcatact ggcttttttc tgccgggcgc ggatacgtat
    11161 ccagcgggaa acaggcgctg atatggtcat gactgctgat gagttttgcc atattcagat
    11221 ccagcgattc agcccgccac atcgtcagat atttctgcga ctgcgcgatg gtcttttcag
    11281 ccttgcgcaa atcagcttcg atttttttga tacgacgcca gcgaacgtcc ggacgttctt
    11341 tatacttcgc ctgaaggagt gccgaccgcg cccgctcctc ccagtattcc gcacgttcaa
    11401 agagcatcac ggctcgtttc atgccgtttt caatacgctg cgcatcacga cgggcgcggc
    11461 gttcgctgtg atgccccaca agaatgggct gaccgggcgg gatcatcgcg gccagtcttt
    11521 ccacttcatc aagtgcctgt gcggattcac tggcccgctt tccgctgtat ccggtaaacc
    11581 gctccgcccg tgcttcctga cgttcagcga gcgtgctgtc ttcatcctca atctctccgg
    11641 caagtgagag gagcacgtct tcccggcccg gtgtccaggc cggtgccaca aacagcgcct
    11701 gtttgggtgc ccagcggaaa ccggcatcat gcactttttt atacgtttcc tggtcaagac
    11761 gtgacgcggc atacaggcgc agtttgttgt catccggtga gtaggtcgcg cggtacatct
    11821 gcacagggct ggcggcttcc ggggcggtca tggtgattgc tgctaatgtc ataaaatgtt
    11881 ctccttccgt ctgttgccgc ccctcgcggg gcggctcacc tcatcagtta aaagcgcgtt
    11941 tcagttcgag tgtgcggagc acctgcccga aggccgtgtt tttcatggcg gggatgtgta
    12001 caccacgccc ccggtgaaaa ctccgggcaa tgtgtcgccc gaaggctttc agcacggggt
    12061 caatttccgg cgcggggatg tcttcgcgtc tgaaacgggg atgacggcgg ggctttacag
    12121 ccgctgcgga aggtgaaaca atacttactg tcataaatga aactccttat gacaaattgg
    12181 ttaataatgc cgtcaaaatt ggttgcgcaa ttcttacggc gtggattaag caggtccctg
    12241 acctgctttt ttcatatctg cgtctgacgt tctccggtgt tttcctcctt ctgttcctgc
    12301 gttctcatta cctgccagcc ggatgactga cagaacaaat aaatgcatat tgccggagcc
    12361 tgaaataaat cctgattttt aaagaacatc acccgttgag tttcgggagc cgcggttttg
    12421 acgtttcgcg ggaacctgct gctgtattcc ctgtggtatg aacttcaccc gtttgagaac
    12481 cttcgccgca aggggcatca ctgccacggc ctgacgcacc tgtcctgggc agaacgcaaa
    12541 attttttacc gggttttggg taaaaaattt tgtgggctgt tcatttaccc tggacagtca
    12601 gtgcggcagg ttgtggctgt atcagccccc ggcgacaggg actcatacgg gtgtgtggca
    12661 cccggcaggc aatgagcagg gacccgccgc aacgacgaag agcgcgtgcg cgaaacggcg
    12721 ttgcccctta catcccggcg ttttttgccg ggatgccggg agcgcaggtc acggcgcgac
    12781 agaggcggcg cgaccggaac ggtcgctgca agccaggggt tttcacgcat cggaccgtag
    12841 cgacagcccg aaaccgcgcc agcgggttcg gcggagactg gcaggtgcgc cagcagctgc
    12901 ctgtcggagt cgggcctgac gaccggatgc cgccaggcag ccgggagccg aaggtgcgcc
    12961 cggctgacag aacaacactg cagtagtcag aatcagagca gcagataaag gaacaacaac
    13021 atcaggaaca gttccataac ataagaaaga aacactcctg agtcagacac atcacaaacg
    13081 ggaagggtaa cgggcgacgc gtcagcgtgg cccgcttccc ttccggcagg cgtcagcctg
    13141 ccagggggtt tcgcgcggac tgacaggcgg cctccaccgc ctgcccctgt atcacctgtt
    13201 tctgctgccc tgtaagatgt agtgaccgcc caggaaaacc gcctgctgat tcgccacaaa
    13261 atcgcagatt tcatcaacaa tctgcgcggt ggtgagcggt ttctgcccca tttcacgacg
    13321 ccgcgcatta atcacggctt tgtaattgcg cagaatttcg cgggtttctg tgctgatatt
    13381 catgacatgt tctccccgtc cagcgcctgt aacacaacgt ccatcgcttc gcgttcttcc
    13441 ctgacgaatg cgtcaccgtc gcccccgttc tcactcccga cggcatacag gcgcttcatt
    13501 tcactgcctg ccagcgccat cactttttcc agcatcaccg ccgggactgt cacctgacgg
    13561 ctggcgcgct tttcttccat cagttcacga acaacgtcgt gaacaacgct gacatccgca
    13621 ccatgctcaa aggcatcgtc cagccgctgc atgacggttt ccagctcatc gtctgacagg
    13681 ttccagtcct gggctatgtg acgcacatcc ccacgggtca gcaccacggc aaaaacgcat
    13741 tcactgtccg gcacgtctgt atccagcact tcgcggataa tttctttaac agtacagtac
    13801 atgttctcgt ctcctttaca ggggcggcat tgccgccccg tccgttaatc agtccagagc
    13861 cgcgtaaatc gcgttgcatt cagggtgaaa ttcgatgtga cgccacaact gcgcatcgcg
    13921 catccggtaa agctgggtca gttctgcatc accgctgtcg tggtacagcc acaactggcg
    13981 gttaatcaca agggaagtca gaatgatgcc gcaggcatca gcactcacgg tacggtcaaa
    14041 ccagtttgca ccgttcacca tgtggaaacg gtcgccatcc ggcatcatgt aaccgccacc
    14101 gtccggcagg cggatgaagt gccagaagcc tccctgatat tccggcatca ttttttgcgc
    14161 cagcgcgtac acatacgcct cggcatacag aaaatcgttg ccaaacaggt aaggcagaaa
    14221 ggagagacgg tcgcactctt caacattcag agtgacaggt ttcgcatatt gcatgatgtt
    14281 ttcctccgtt gttcacatat tccgcgacac cgggcggtgc cgtgcctcca gaaaagccgg
    14341 gcgataaggc ggggatgcac agggcgcaag ggaagccgca gcctgacgca cacggctgac
    14401 gggagttgca gggtgaggcc gcgaaacctg agcgggcgaa cgaccggaaa atcacggcag
    14461 acgggaagga gggaccgggt gaccgcagcg ttcattttac ccttgcatcc ccgtcagccc
    14521 ggctaagatg gaggcacggc acagcccgca cacgacacgg tggccacata actgaaaacc
    14581 cacaaggcca tgccggcatg gtcgggtaaa gcggagccgg cggcgacttc ctttgtgctc
    14641 tgtccgggga ccggcagaaa aaaagcgggg attttccccg ccatcatcag cccctgattt
    14701 ttgccagggc atccgccatg ctgcggattt cggcccgggc ttcccggaca acttcccgca
    14761 gaacaccacg gtcaaaccac cggcggggcg gttcatcagg tatacgcagc acatcgcagg
    14821 ccagcccact ggccaggata tgttcaatac aatcgctgac cagatacatt tccaccgccg
    14881 aaaaacgtgc gccccggttc aggatggcgt caagcaccac gggcaggttt tcgtcgtcct
    14941 catcgagcca gcaggggtga tcgctttcct tcacgaccag gcgaaagcgg cgcgtctggc
    15001 gggtcagtaa cgtctgaccg gttgtggtca gcgccgcccg gatacgccgc gcatcttctg
    15061 ccacccggca actgacactg catactgcat cgcgcaccgc atcaagaact gccctgattt
    15121 cgtcctctgc cagccactca ccggcatacg ggaaagacac ctgcgttccg tctgccggaa
    15181 gtaccggcag cgactgccac tgactgccgg acagaacaag ttccgctccg gttccggtca
    15241 gtttctgaat ggcggaggcc aggagcggcg gcagtacgcc gccgtgacgg ttgataagta
    15301 aggtcgccag ccggggcgca tccagcagaa cataaatgct gatccagccc ggatacttcg
    15361 ggttacgctg gtcaacatcc gtctcttccg gccagccctg cgccagcagc aggttgcggg
    15421 cgtgacgaca aatcagtgca ttcagcgttt cgttcatgat tcatggtctc cctgaatcgc
    15481 cgcccgcagg cggcgataac atcagtcgtt ttcgcgtgtg ttgtttttca gcagggtgcg
    15541 ggcctgatta accaccgcct gacggcgggt gacgcattcc gggacggcga ggccatgatc
    15601 ccgggcggcc cattcacgtt tccacaatgc ctgttcagcg gtctgaatca gggacgccag
    15661 cagttcagcc ggaacggtca cctggcggtt ttcgctcacg ttgctgataa tctccatcgc
    15721 cgcggcggaa gaaataccgg actcaattcg ctggtcttcc gggatgtcct ccagccgcgc
    15781 caggacggtc ctgatttcat gatcggacag ggaaatatcc attccgtcag cgagggcctg
    15841 gatctcttcc ggggaccaga caaccagcat cagcggcgtg tctcccggat actgcgctgc
    15901 cagctcccgg cagagcgttt cgcatgttcc gtacattctc gtctccttct caatctttga
    15961 agcaggaatc cagttccaga atttcggtat acgcctccat aaacaggcga ttggcgcgat
    16021 acagcgtctg gcgtgtcagg cgttcaacaa gcacatcgga atcaaagcga cagcgatgct
    16081 ccatatcgtt cagcctggcc cgaaggtcac acatggtgtt ggccacctcc gccacgtcgg
    16141 cgcgaagttt ttcgtgtccg gcatagttca tttacgcagc ctccggttca aaccagttat
    16201 cattcgcggc cccctgctgc atggcccgtt gcacggcggc cagtcgttgc tgtccggcac
    16261 ggtgatactg ctcaagcagc tcgataccga tataccggcg tccggactgg agggcggcca
    16321 cacaggttga gccgctgcct gcaaacgggt ccagcacaat cgcgttcgga tgtgtgaagc
    16381 tctcaatcag tggttgcagg ctggtaacgg gcttttccgt cgggtgatgg cggttgcctg
    16441 aatatttcca gcccagcacg tcgggcaatg gcttctgagg cagcgccgga cgcccttttg
    16501 ccaggatgta agcgcattcg tggcgatagc ccacatatgc ggccttcgat gtgtaggttt
    16561 tggtaaatac caggtggcca accacgctga atcccgcatt tttccaggcg gccataaagc
    16621 gatcgacgcg gttccagcca tagaagctca ccatcagcgc atcttttttc agcacgcggt
    16681 acatttcatt acaggccggt tgcagccatt catcggtttt atcgccggcg atggtacgcc
    16741 cctgacggtc acggaaaccg acgagatatg gcgggtcggt gaggatgaaa tcgacagcat
    16801 tgcccggaaa cgtggccata acacgcacgc agtcaccctg gataaaacgg gacataacag
    16861 cactccttct cttacagagg gcaccgggag ggcgcccctg tggcgcccgt tccccgctgc
    16921 ctgtgggctg ttcaccgcgt ccggtaacgg gtcaggccga ccgtggaata ccgcagccgt
    16981 cgtctttttg acggcgagga tatgccgcga aagcggcttg aagggcgttc atcagaaaag
    17041 ggcgacgggc tggcgtacac tgtcaccaac gccagccggg agcggtaaaa gcgcccgtcc
    17101 cgtgcccggc cggacgcggg acactgacca caggcagcgt tcctgccgcg ccgcgtcagc
    17161 cggcgcagta aaaagaggag cccggcgaca tcttttgtca cccgccaaac aaaacccaaa
    17221 aacaacccac accaaaccca ataaaacacc aaaacaagac aaataatcat tgattgatgg
    17281 ttaaaatggg gtaaacttga caaataaacc cacttaaaac ccaaaacaca cccaaataca
    17341 caccaaaaaa aacaccataa ggagttttat aaatgttggt attcattgat gacggttcaa
    17401 caaacatcaa actacagtgg caggaaagcg acggaacaat taaacagcac attagcccga
    17461 acagcttcaa acgcgagtgg gcagtctctt ttggtgataa aaaggtcttt aactacacac
    17521 tgaacggcga acagtattca tttgatccaa tcagcccgga tgctgtagtc acaaccaata
    17581 tcgcatggca atacagcgac gttaatgtcg ttgcggtgca tcacgcctta ctgaccagtg
    17641 gtctgccggt aagcgaagtg gatattgttt gcacacttcc tctgacagag tattacgaca
    17701 ggaataacca acccaatacg gaaaatattg agcgtaagaa agcaaacttc cggaaaaaaa
    17761 ttacattaaa tggtggggat acattcacaa taaaagatgt aaaagtcatg cctgaatcta
    17821 taccagcagg ttatgaagct ctacaagaac tggatgagtt agattcttta ttaattatag
    17881 acctcggggg caccacatta gatatttctc aggtaatggg gaaattatca gggatcagta
    17941 aaatatacgg agactcatct cttggtgtat ctctggtcac atctgcagta aaagatgcac
    18001 tttctcttgc gagaacaaaa ggaagtagct atcttgctga cgatataatt attcacagaa
    18061 aagacaacaa ctatctgaag caacgaatta atgatgagaa caaaatatca atagtcaccg
    18121 aagcaatgaa tgaagcactt cgtaaacttg agcaacgtgt attaaatacg ctcaatgaat
    18181 tttctggtta tacacatgtt atggttatag gcggtggcgc agaattaata tgcgatgcag
    18241 taaaaaaaca cacacagatt cgtgatgaac gttttttcaa aaccaataac tctcaatatg
    18301 atttagttaa cggtatgtat ctcataggta attaataatg gacaagcgca gaaccattgc
    18361 cttcaaacta aatccagatg taaatcaaac agataaaatt gtttgtgata cactggacag
    18421 tatcccgcaa ggggaacgca gccgccttaa ccgggccgca ctgacggcag gtctggcctt
    18481 atacagacaa gatcccaggg cccctttcct tttatgtgaa ctgctgacga aagaaaccac
    18541 attttcagat atcgtgaata tattgagatc gctatttcca aaagagatgg ctgattttaa
    18601 ttcttcaaca atcactcaac cctcttcaca acaggagcaa agaagtgatg aagagacaaa
    18661 aaaaaacgcg atgaagttaa taaactgatt aaatttttat tgatatcttt acccccataa
    18721 acggggtaaa ggtatcaaca aacctatttt ttagctatgt acagattaca tctgacaagt
    18781 tatttctacg taacaaatca cagtctaaaa atcagtatag ctttaccctt tgcactgttc
    18841 agcttattcc tttcagaaaa aaaacgggaa catcagttac tttataaatc tgcataataa
    18901 gtagtattct atgcctgcca tctataatta ctttcccgtc ttcggacatt tgtggatatt
    18961 caatcgttcc ttgacttaaa taggttttaa ttttgtgcca tcggcgtgga tcgatattga
    19021 aagcctgaac gccaagcgac cttaacaggg ttaacccatt aatcatcgtg accacaggct
    19081 tttgtctcat tctctcagga agttgatggc aattagcagc cagggccgcc gggggaaaat
    19141 aaaactccag ttcctgtagt gaagggtatt gcaaaccact gttgtccaga ggaatatcat
    19201 caaaggattt gaaaggatac ttggacatat acgcattgat tttttcaggc ggtaaatgtg
    19261 caaaccagtt aggatcaggc ctgttcgctt cttcttcttc tttaattcgt tgaaatgact
    19321 ccggaaacaa atcttccaca cttatgttca tcacaatatc ctcccttgag agcattgctc
    19381 atcgccaccg ataaggattt cattataaaa cacgccagta cagaacaact cactggtttc
    19441 aaactcccgt cgtccgtcct gaccggtacg gtaactaaca agacctgtac acatatcacg
    19501 atgtgtacca tgccataaag tgacagtatc cccccaagtg tctcatatca tagtttcatt
    19561 aaaacatgct gaggccagag aacagttatg cctgtgccgt tttttgtggt atcaggcgag
    19621 aatagcacgt ggtgtgaatg cctgtttttt gatcgttttc acaaaaattg aaatccacag
    19681 tcttgacaga gaaaacacag cggcgtaatt tttgatctgt acataaaacc agtggttata
    19741 tgtacagtat ttaattttaa ttgattgttt ttaaagtcaa agaggttttc tatgagtgat
    19801 aatgaaggta gtgtaccgac agaaggtatt gattacgggg acacaatggt tgtgtggccg
    19861 tcaacaggac gaattccggg cggtgatgtg aaacccggag gctcatcagg tctcgctcca
    19921 tccatgcctc cgggatgggg ggattacagc ccacaaggta tcgcacttgt acaaagtgtt
    19981 ctttttcctg gaattattcg ccggattatt cttgataaag aacttgaaga gggagactgg
    20041 tcgggatggt ctgtcagtgt gcatagcccc tggggaaacg agaaagtttc cgctgcacga
    20101 acagttcttg a
//
LOCUS       DAZFKF010000093        19932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFKF010000093 DAZFKF010000000
VERSION     DAZFKF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.093"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..226
                     /locus_tag="ACHKA1_003900"
     CDS             <1..226
                     /locus_tag="ACHKA1_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681485.1"
                     /translation="TYLIDPKINYNTDLNIEDSSSEEFLSDNTLEKDENSPDNCFEVV
                     KYNTYEAYNSEKQYFTREDYTYDYDLLNAI"
     gene            complement(618..994)
                     /gene="rnpB"
                     /locus_tag="ACHKA1_003901"
     ncRNA           complement(618..994)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKA1_003901"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1027..2172)
                     /gene="garK"
                     /locus_tag="ACHKA1_003902"
     CDS             complement(1027..2172)
                     /gene="garK"
                     /locus_tag="ACHKA1_003902"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO5681486.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2269..3159)
                     /gene="garR"
                     /locus_tag="ACHKA1_003903"
     CDS             complement(2269..3159)
                     /gene="garR"
                     /locus_tag="ACHKA1_003903"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO5681487.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVVLGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3189..3959)
                     /gene="garL"
                     /locus_tag="ACHKA1_003904"
     CDS             complement(3189..3959)
                     /gene="garL"
                     /locus_tag="ACHKA1_003904"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO5681488.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3975..5309)
                     /gene="garP"
                     /locus_tag="ACHKA1_003905"
     CDS             complement(3975..5309)
                     /gene="garP"
                     /locus_tag="ACHKA1_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO5681489.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSGEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5684..7255
                     /gene="garD"
                     /locus_tag="ACHKA1_003906"
     CDS             5684..7255
                     /gene="garD"
                     /locus_tag="ACHKA1_003906"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO5681490.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7404..7739
                     /gene="prlF"
                     /locus_tag="ACHKA1_003907"
     CDS             7404..7739
                     /gene="prlF"
                     /locus_tag="ACHKA1_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO5681491.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7739..8203
                     /gene="yhaV"
                     /locus_tag="ACHKA1_003908"
     CDS             7739..8203
                     /gene="yhaV"
                     /locus_tag="ACHKA1_003908"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO5681492.1"
                     /translation="MDFPQRVNGWALYTHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(8258..9067)
                     /gene="agaR"
                     /locus_tag="ACHKA1_003909"
     CDS             complement(8258..9067)
                     /gene="agaR"
                     /locus_tag="ACHKA1_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO5681493.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRLQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9316..10596
                     /gene="kbaZ"
                     /locus_tag="ACHKA1_003910"
     CDS             9316..10596
                     /gene="kbaZ"
                     /locus_tag="ACHKA1_003910"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO5681494.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPV
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10619..11092
                     /gene="agaV"
                     /locus_tag="ACHKA1_003911"
     CDS             10619..11092
                     /gene="agaV"
                     /locus_tag="ACHKA1_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO5681495.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            11103..11882
                     /gene="agaW"
                     /locus_tag="ACHKA1_003912"
     CDS             11103..11882
                     /gene="agaW"
                     /locus_tag="ACHKA1_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO5681496.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11872..12750
                     /gene="agaE"
                     /locus_tag="ACHKA1_003913"
     CDS             11872..12750
                     /gene="agaE"
                     /locus_tag="ACHKA1_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO5681497.1"
                     /translation="MASNQTTLPNISENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTMTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12768..13202
                     /gene="agaF"
                     /locus_tag="ACHKA1_003914"
     CDS             12768..13202
                     /gene="agaF"
                     /locus_tag="ACHKA1_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO5681498.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            13199..14332
                     /gene="nagA"
                     /locus_tag="ACHKA1_003915"
     CDS             13199..14332
                     /gene="nagA"
                     /locus_tag="ACHKA1_003915"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO5681499.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRYLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQGQLASF"
     gene            14683..15837
                     /locus_tag="ACHKA1_003916"
     CDS             14683..15837
                     /locus_tag="ACHKA1_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO5681500.1"
                     /translation="MPENYTPAAAASGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFAVL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15850..16710
                     /gene="kbaY"
                     /locus_tag="ACHKA1_003917"
     CDS             15850..16710
                     /gene="kbaY"
                     /locus_tag="ACHKA1_003917"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO5681501.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16877..17353
                     /gene="agaB"
                     /locus_tag="ACHKA1_003918"
     CDS             16877..17353
                     /gene="agaB"
                     /locus_tag="ACHKA1_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO5681502.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17392..18195
                     /gene="agaC"
                     /locus_tag="ACHKA1_003919"
     CDS             17392..18195
                     /gene="agaC"
                     /locus_tag="ACHKA1_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO5681503.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            18185..18976
                     /gene="agaD"
                     /locus_tag="ACHKA1_003920"
     CDS             18185..18976
                     /gene="agaD"
                     /locus_tag="ACHKA1_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO5681504.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18977..19732
                     /locus_tag="ACHKA1_003921"
     CDS             18977..19732
                     /locus_tag="ACHKA1_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO5681505.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQHVDVSQLTVVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTSIPASFLWLHSNFICLINT"
BASE COUNT         4876 a         5165 c         5030 g         4861 t
ORIGIN      
        1 tacttatctt atcgatccaa aaattaatta caatacagat ttaaacatag aggattctag
       61 ctcagaggaa ttcctttcag ataatacact cgagaaagac gaaaattctc ctgacaattg
      121 ctttgaggtt gtaaaatata acacatatga agcatataac tcagagaaac aatattttac
      181 cagagaggac tatacgtatg attacgacct tttaaatgca atatagactt aataacttct
      241 tacaaagtct attattaata ccgtagatat tcatcttacc ctgtgcacca aaagcaaaaa
      301 cttatcgacg cgggtttgaa actgatggca accgcaaaaa tgcctgatgc gctacgctta
      361 tcaggcctac gccatctctg caatatattg aatttgcgtg cttttgtagg caggataagg
      421 cgttcacgcc gcatccggca tgaataaagc gcactcaaca gtctgaaacc cgccggaacg
      481 gtttattacg tacatcaggt gaaactgacc gataagccgc tttcttttgg gtctagcgtc
      541 gtggacagtc attcatcttt ccgcccctcc aaaagcaaaa acccgccagt ttacgctggc
      601 gggtttttac gtaaatcagg tgaaactgac cgataagccg ggttctgtcg tggacagtca
      661 ttcatctagg ccagcaatcg ctcactggct caagcagcct acccgggttc agtacgggcc
      721 gtaccttatg aacccctatt tggccttgct ccgggtggag tttaccgtgc cacggactgt
      781 taccagccgc gcggtgcgct cttaccgcac cctttcaccc ttacctgatc ccgcttgcgc
      841 gggccatcgg cggtttgctc tctgttgcac tggtcgtggg tttccccccc aggcgttacc
      901 tggcaccctg ccctatggag cccggacttt cctcccctcc gcccgtctcc cccgaagagg
      961 acgacgacga agcggcgact gtctggtcag cttcggcgcg cagtatagag ggtttgcgcg
     1021 cccttgtcac cccgcgttgc gcattccaat cgccagtgtc gcggcgatat tacgtgaagc
     1081 acggcagata ttgtcataag ccccgcggaa tgcttcgtcc aacgtaccta tgctggtcaa
     1141 tacgctgaag accgcatcaa tgccatgctg atgtacaacg ccaacatcat cggtcaggct
     1201 acccgcaatg ccaatcaccg gtttatggta cttcttcgcc acgtttgcga caccaatcgg
     1261 taccttgccg tgaatactct ggctgtcaat acgcccttca ccggtgatca ccagcgtaca
     1321 atcgtgaata tgttcctcca gattcagcgc cgtagtgacg atttcaatac cacttttcag
     1381 ttccgcacca agaaacgcca ttagcgccgc gcccatacca cccgcagctc ctgcgccggg
     1441 gacatctttc acatcaacat gcagcgcttt tttaatgacc tcggcatagt gagagaggtt
     1501 attgtccagc tcaacaatca tcgcttcact ggctcccttt tgtgggccaa agatgcgcga
     1561 tgcgccgtta tcgcccacca gcggattggt gacatcacaa gcgacgcgaa tgacgcaatc
     1621 ttttaagcgc ggatcgaggc cggaaatatc aatatcattc agagtattaa gactaccgcc
     1681 gccaaaacca atttcattgc cgttggcgtc gcataatttc gcccccagcg cctgtaccat
     1741 gcctgcgccg ccatcatttg tagcgctgcc gccaatgccg ataataatgt ttgtcgcgcc
     1801 gctctccagc gcctgcagga ttaactcgcc tgtgccgcgt gaagtggtca cgagtggatc
     1861 gcgtttttcc gcaggtacca gctccagccc actggccgcc gccatttcaa taaacgcggt
     1921 tttgccatcg ccggagatcc cccaactggc attcactttc tcgcccagcg gccctgtaac
     1981 ccaggcgtga cgttcggaac cctgggtggc tgcaatcatc gcttccaccg ttccttcgcc
     2041 accgtcggca acaggaacag aaacgtactg tgcatcagga aaaatttccc gaaatccttt
     2101 ttctatcgcc tgcgcaacct cgctggcaga taaactttct ttataagagt ctggggcgat
     2161 tacgattttc atacctatgc ctgttaccac atgacgccgg agggcgtttc tcttattcgg
     2221 cctggattcc aggcccggat tgcaatacgc catccgggca cgacgtcatt aacgagtaac
     2281 ttcgactttc gccagttttt cgtagtagca cgccagggcg ctatgatccg ccgttcctaa
     2341 accatctgca cgcagtgcct gcatcatctc cataaccgca gctgtgagcg gcagttgcgc
     2401 gccgacaccg tgagaagtat ccagcgcatt cgccagatcc ttaatatgca gatcaatacg
     2461 gaagcccggc ttgaagttgc ggtccatcac catcggcgct ttggcatcca gcacggtact
     2521 gcccgccagc ccaccacgaa ttgcctgata aaccaggtcc gggttaacgc ccgctttagt
     2581 tgccagcgtt aacgcttctg acatcgcggc aatattcagc gccacaatga cctgatttgc
     2641 cagtttagtg acgttacctg caccgatttc cccggtatgc accacggaac ccgccatcgc
     2701 tttcatcaaa tcatagtatt tgtcgaaaat agccttgtcg ccgcccacca tcactgacag
     2761 cgtaccgtcg atggctttcg gttcaccgcc gctcaccgga gcatccagca tatcaatgcc
     2821 tttcgctttc agcgcttcgc tgatttcacg gcttgccagc ggtgcgatag aactcatatc
     2881 gatcaatacc gtacctggct tcgcgccttc aataatgcca ttctcaccca gcaccacctc
     2941 tttcacatga ggggagtttg gcagcatggt tatgatgacg tcgcactgtt cagcgatcgc
     3001 tttagccgta gaagctgttt ctgcacctgc agcaatcacg tcagcaatag cttctgggtt
     3061 acggtcagca accaccagcg agtaacctgc tttcagaagg tttttactca ttggtttacc
     3121 cataatcccc aggccaataa aaccaacttt catagtcata tcaatcatct ctcttgttgc
     3181 ggtggtggtt attttttaaa ggtatcagcc agtttctgag tggcagagcg gaagacgccg
     3241 agatcgctgc cgacagccac aaacgtcgcg ccccattcca gataacgacg cgcatcggct
     3301 tcgaccggcg cgaggatacc gctgggtttg ccgtgcgcgc tggcacggtt aaaaatgtgc
     3361 tgaattgctt tttgtacatc cgggtgtgat gcattgccga gatggcctaa tgccgcggcc
     3421 agatcgctgg ggccgacgaa gatgccgtct acgccttcgg tagcggcaat agcatcgacg
     3481 ttatctacgc cctgctgact ttctatctgg accagaatag tgatgttctt gttcgactga
     3541 gcgaaataat ccgccacggt gccaaacata ttggcgcggt gagaaacgga gacgccgcga
     3601 atgccttctg gcggataacg cgtcgatgcc accgcctgct ctgcttcctc ttttgtttct
     3661 acaaaaggaa tcaggaagtt atagaaaccg atatccagaa gacgcttaat aattaccggc
     3721 tcgttggtcg gcactcgtac tactggcgcg ctggcgctgc ctttcagagc cattaactgc
     3781 ggaataaacg tggagatatc gtttggcgca tgttcgccat ccagcaccag ccagtcaaac
     3841 ccagccaaac caagaacttc agtgctaatc gggttagaga gtgctgacca gcaaccaatt
     3901 tgtacctgtt tcgcagccaa tgcggctttg aatttattcg ggaaaacatc gttattcatc
     3961 gcttatacct ttgcttattt ctgcaattcc atacgtttaa tgtcgccaac tacgaagagg
     4021 tagcagacca tcgccatcag cgctgaacat cccacgaaaa ccagtgctgc attgaaggag
     4081 tgcagttcac ttaccaggta gccaatcacc agtggagtga caatggaggc aacattgcca
     4141 aagacgttaa agacgccgcc gcagaggcca acaatctctt tcggcgcggt gtcagaaatc
     4201 accggccagc ccagcgcacc aaatcctttg ccaaagaaag ccagcgccat cagcatgacc
     4261 accagcgtgg tgttgttggt gtagttacat aagatgatgg tggaagccag caacattccc
     4321 agcacaatcg gtagcttacg tgccagggtc agggataaac cgcgtttgat cagataatcc
     4381 gagaagacac ctcccagcac gccgcccgca aaaccacaca gtgctggaat cgaggcgacc
     4441 agacccactt tcagaatcga catgcctttt tcctgcacca gataaatcgg gaaccaggtg
     4501 aggaagaacc aggtgatggt gttgataaaa tattgtccga aaaatacgcc caacatcatg
     4561 cggttagaga gcaattgctt gatgtaatgc agtttgggac cgcttgctgc cgcgctgccc
     4621 ggctttttgt ggtccatatc gaccaccgcg ccattttcag agataaactt cagttcttcc
     4681 ccagacatac gtgggtgatc tgtcgggtta tgaatcaatt tgatccacag cgccgtcagc
     4741 acaaaaccaa tcacccccat aacggtaaag acgtgctccc agccccaggc gaaagtcagc
     4801 cagccaagca gaggcgaaaa gagcgccagc gagaaatatt gcgccgagtt aaagatggcg
     4861 gaggcagtac cacgttcttt cgtcgggaac caggcggcga caatacgggc gttcgccggg
     4921 aatgatggcg cttccgagaa gccgagcata aagcgcataa agaacatgga gatccctgcc
     4981 caggccagcg ggaacatatc aacaaagcct tgcaggaagg tgaatagcga ccagaaaaag
     5041 aggctgtagg tgtaaacttt tttcgagcca aacttatcaa gcagccagcc gccggggatt
     5101 tgcatcagca agtaggccca gccaaaagca gagaagatgt aacccatcga aaccgcactt
     5161 aactgcaact cttttgccac ttcggtacca gcaatagaca gcgttgcacg atcggcgtag
     5221 ttaacggcgg taacaataaa aataatcagt aatattaaat agcgggtatg cacgcctttc
     5281 tttttttcgt caacggtgtc cagaatcatt ttatttacct cgggtactta tgctgatttt
     5341 tattattatg gggaaggtgt tatttatgag tttcatttat gccgtaacga caatgaactc
     5401 gggaattagt ataagcagcg cgagaataat aatcattgtg caaatgctaa tttaattaat
     5461 actatttaaa tattattttg agcatatgca cataaggttg cgcgctaaag cacagatttg
     5521 cgctttacct tgccgggcgg cattgcaatc cctgaaatga ttgacattga tcacatttct
     5581 gcatttaaac tcctgacatt cttatttcac ccaatgaagt catttatttt taaatgagac
     5641 caggtcctca ttttaataac ccctggctgg agaatattgc acaatggcca acatcgaaat
     5701 cagacaagaa acgccaactg cgttttatat aaaagttcac gacacagata atgtggcaat
     5761 tattgttaat gataatggcc tgaaagcggg aacgcgtttt ccggatgggc tggaattaat
     5821 tgaacatatt ccccaggggc ataaagtcgc attgctggac attccggcta atggtgaaat
     5881 tattcgttat ggcgaagtga ttggttacgc cgtgcgtgca atcccacgcg gaagctggat
     5941 cgacgaatca atggttgtac taccggaagc gccgccgtta cacacgctgc cactggcaac
     6001 caaagtcccg gaacccttac cgccgctgga aggatacacc tttgagggct atcgcaatgc
     6061 cgatggcagc gtgggcacca aaaacctgct cggcatcacc accagcgtcc actgtgtggc
     6121 aggcgtggtg gactacgtag taaaaatcat tgaacgcgat ctgctgccga aatacccgaa
     6181 cgtcgatggc gtggtggggc tgaatcatct gtacggttgt ggcgtggcga ttaacgctcc
     6241 ggcggcagtt gtacctatcc gtaccattca caatatttcg ctgaatccta actttggcgg
     6301 cgaagtaatg gtgattggcc tgggttgtga aaagttgcag cctgagcgcc tgctgactgg
     6361 aacggatgat gtgcaagcta ttccagtaga aagcgccagc attgtcagtt tgcaggatga
     6421 aaagcatgtc ggttttcagt ccatggtcga ggatattttg caggtcgccg aacgccattt
     6481 acaaaaactg aatcaacgtc agcgagaaac ctgtccggct tcagaactgg ttgtcggcat
     6541 gcagtgcggt ggcagcgatg ccttttctgg cgtaacggca aacccggcgg ttgggtatgc
     6601 gtctgatcta ctggtgcgct gcggcgcaac ggtgatgttc tcagaagtca cggaagtgcg
     6661 tgacgcgatc catctgctga caccacgcgc agtgaacgaa gaggtcggca aacggctgct
     6721 ggaggagatg gagtggtacg ataactatct caatatggga aaaaccgacc gcagcgccaa
     6781 cccttcgccg ggcaacaaga aaggcggtct ggcaaatgta gtggagaaag cgctcggctc
     6841 cattgctaaa tcgggtaaaa gtgcaattgt tgaagtgctg tcgcccggtc aacgcccgac
     6901 taaacgcgga ttaatttacg ccgcgacgcc agccagcgat tttgtctgtg gcacgcaaca
     6961 ggtggcttcg ggtatcaccg tgcaagtgtt tacgaccggt cgtggtacgc cgtacggcct
     7021 gatggcggta cccgtcatta aaatggcgac ccgcaccgag ctggcgaacc gctggtttga
     7081 tttaatggat attaacgcgg gcactatcgc caccggcgaa gaaaccattg aagaggtggg
     7141 ctggaagttg ttccacttta ttctcgacgt cgccagcggg aagaagaaaa ccttctcgga
     7201 tcaatgggga ctgcataacc agctggcggt gtttaaccca gcaccggtga cctgatttct
     7261 tttggaacgt ccctcgcaaa acatggcctt agtgccatgt ttttattgtt taaagccccc
     7321 acgtccatta ataatgcatt tgcattacct tagttcaagc ttataatttg agcagaaaac
     7381 aggctgtaaa aggacagtga atcatgcccg ctaatgctcg ctctcacgct gtactgacca
     7441 ctgaatcaaa ggtcacgata cgcggacaaa caactatccc cgcgccagtg cgtgaggcct
     7501 taaaactgaa gccaggccag gacagcattc attacgaaat tctgcctggt gggcaagtat
     7561 ttatgtgccg actgggagat gaacaggagg atcatactat gaatgcattt ttgcgttttc
     7621 tggatgcaga tatccagaac aacccgcaaa aaactcgtcc attcaacatt caacaaggaa
     7681 agaaacttgt cgctggcatg gacgtcaaca ttgatgatga gattggcgac gacgaataat
     7741 ggattttcca caaagggtta atggttgggc gctatatact catccctgtt ttcaggaaac
     7801 ctacgacgct ttagttgccg aagtcgagac attaaaggga aaagatcctg aaaattatca
     7861 gagaaaagcc gccacaaagt tattggcggt agtccataaa gtgattgagg agcatatcac
     7921 ggtcaatcca tcatcaccgg cattccgtca tggcaagtcg ttaggctctg ggaaaaataa
     7981 agactggtca cgggtaaaat ttggtgctgg tcgttatcgt ctcttctttc gttatagtga
     8041 aaaagagaaa gtcatcattc tgggatggat gaacgatgaa aacacgctgc gcacctacgg
     8101 taaaaaaaca gatgcctata ccgtattcag caaaatgtta aaaagaggac atcctcctgc
     8161 cgactgggaa accctcaccc gagagacaga agaaacccat tgatggtgtt cacaatgccc
     8221 gccgaacgca ttcgtgttga ccagcgggca ccctttctca ctccccgacc agaatcactt
     8281 caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca tcaacaatga
     8341 tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggagcggttg aacttactgg
     8401 aatcggtgac tacgatgatc cgttccgcaa cttcgcacat ccggcggttt aaacgggctt
     8461 catcttcatt atgcgtgctg acgccgcgct ccagatcaat cgcatctaca ccaagaaaca
     8521 gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa aaagattgcg
     8581 actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct tccagcaaag
     8641 cattagccac gttcataccg ttggtcatcg caattacgtc agtgtgcttg cgcatcagac
     8701 gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct ggctgaatca
     8761 actcaacggc agctttcgca acgctgcgtt tcattgcggt gttcagcgcg cttttatctt
     8821 ccactgatgg ctcgactgac ggcgttgtgc tatcgcagat caacgcgcca ccataggcac
     8881 gcacagcgat cccctgcttt tccagaaacg ccagatcgtt gcggatcgtc acagtagata
     8941 cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgcagtcgc agacgctgaa
     9001 tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa gcgtcggtat
     9061 tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct attaacgcct
     9121 ttctattaag caaatgcaag cccaccttgc ccattgacgc aagctactct cgtttcagtg
     9181 actttcatta tgtttctttt gtgaatcaga tcagaaaacc attatctttc gttttatttt
     9241 tatctcacca tgacgcagta tcaactgaaa caaaacgaaa gattaatatc gcagtaatct
     9301 gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa agcgggcaaa
     9361 acaaatggaa tttatgccgt ttgttccgca catccgctgg tgctggaggc tgcaatccgc
     9421 tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca ggtagaccag
     9481 ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg tcagctcgcc
     9541 gactcgttga atttcccgca ggatgcgttg attctgggtg gtgaccatct ggggccaaac
     9601 cgctggcaaa acctgccagt cgctcaggca atggccaatg ccgatgattt gattaaaagc
     9661 tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg tcaggacgat
     9721 ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc gaaagtggcg
     9781 gaagaaacct gtcttgaaca ctttggcgaa gccgatctgg agtatgtcat tggtaccgaa
     9841 gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac cacgccggat
     9901 gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg tttgaatgcc
     9961 atctggccac gcatcattgc cctggtggtt caacccggcg tcgaattcga tcacaccaac
    10021 gttattgatt atcagcccgc caaagcgacc gccttaagcc agatggtcga aaactacgaa
    10081 acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatcgct gcgccagctg
    10141 gtgattgacc actttgccat tctgaaagtt ggcccagcgc tgaccttcgc cctgcgtgaa
    10201 gctctgttct ctctggcagc gattgaagaa gaactggtgc cagcaaaagc ctgttctggt
    10261 ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca aagccactac
    10321 cacggtgacg gcaacgcgcg tcgtctggcg cgtggttata gctactcgga tcgcgtgcgc
    10381 tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg taatctggcg
    10441 gattcaccaa ttccgctgcc gctgatcagc cagtatctgc cgctgcagta cgtgaaagtt
    10501 cgctccggcg agctgcagcc aacgccacgg gaactcatta tcaaccatat tcaggacatc
    10561 ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg aacacgctat
    10621 gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg tcggcgttca
    10681 gtgggtcgga tttgcggggg caaatctggt gctggtcgcc aacgatgagg ttgccgaaga
    10741 tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg tacgtttctg
    10801 gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga aaatcctgct
    10861 ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc cggtgaatcg
    10921 cattaacgtt ggcaatatgc actacgccaa tggcaagcaa caaatcgcca aaacggtttc
    10981 tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg tggaatgctt
    11041 cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt gagggattca
    11101 tcatggaaat cagtctgttg caggcattcg cgttgggcat tatcgccttt atcgctggcc
    11161 tggatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc ccgttagtcg
    11221 ggctggtact tggcgatctg cataccggta ttttaaccgg cggtacgctg gaactggtgt
    11281 ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc ggtactatcg
    11341 tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcagtc ggtgtcgccg
    11401 tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg atgtctggcg
    11461 tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt gaacgcgtaa
    11521 actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc ctgcctatct
    11581 acttcggcgc ggaacatgca aaaaccatca ttgatgtcct accgcaacga ttaatcgacg
    11641 gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg ctgaaaatca
    11701 tgatgaaaaa cgtctacatc ccctacttca tcctgggctt tgttgccgca gcctggctca
    11761 agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc gatctgctgc
    11821 gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa gatggcatct
    11881 aatcaaacca ccctgccgaa catcagtgaa aacgaagaaa cattgctgac tggcgtcaat
    11941 gaaaacgtgt atgaagatca gagcattggc gcggagctga cgaaaaaaga tatcaatcgc
    12001 gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat gcaggcttcc
    12061 ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa acgcgacctg
    12121 gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct ggtgacattt
    12181 gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag tattcagagc
    12241 accaaaattg ccgtcggtgc gccgctcggc gggattggcg atgcaatgtt ctggctaacg
    12301 ctactgccga tttgtggcgg gattggagcc agtctggcat tgcaaggctc cattcttggc
    12361 gcagtcgtct ttattgtgct gttcaacgtg gtacacctgg ggctgcgttt tggtctggcg
    12421 cattatgctt accgcatggg cgtggcggcg attccactca ttaaagcaaa taccaaaaaa
    12481 gtcggccatg ctgcgtctat cgttgggatg acggtaatcg gtgcgctggt ggcaacctat
    12541 gttcgtttaa gcaccacgct ggaaatcaca gcgggcgacg cagtggttaa gttacaggct
    12601 gatgttatcg acaaactgat gcctgccttc ttaccgttgg tctacaccat gaccatgttt
    12661 tggttggtac gccgcggctg gagtccgctg cgtctgattg cagttaccgt ggttctcggc
    12721 atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg ttaagtatta
    12781 ttttgacagg gcatggcggg tttgccagcg gcatggaaaa agcaatgaag caaattctcg
    12841 gtgagcaatc gcagtttatc gccatcgatt ttccggaaac ctcaagcacc gcgctgctta
    12901 catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt gtttttctga
    12961 cggatttgct gggcggcacg ccgtttcgcg tagcttcaac actggcgatg caaaaaccgg
    13021 gctgtgaagt gatcaccggc accaatttgc aactgctact ggagatggtg ctggagcgcg
    13081 aagggttaag cggtgaagaa ttccgtgtgc aggcgctgga gtgtggacat cgtgggctga
    13141 ccagtctggt ggacgagtta ggccgctgcc atgaggaatg tccggtcgag gaaggaatat
    13201 gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggctcg atgaccatca
    13261 gttgcgtatt gctgacggtg tcatcgcagc aatcgaaccg attccagtgg gcgtgactga
    13321 acgcgatgcg gaactgctct gccctgctta catcgacacc catgtacacg gtggtgcggg
    13381 cgttgatgta atggatgacg cgccggatgt actcgacaag ctggcaatgc acaaggcacg
    13441 cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg ccgcttaata ccattcatgc
    13501 ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga cctggtgcgc aagtgctggg
    13561 gagttatctc gaaggaccgt acttcacgcc gcagaataaa ggcgcgcatc cgccggaatt
    13621 gtttcgcgag cttgaaattg ccgagctgga tcaattgatt gccgtttctc agcacacctt
    13681 acgcgtggta gcgctggcac cggaaaaaga aggggcattg caggccatcc gctatcttaa
    13741 acagcaaaat gtaagggtga tgctggggca tagcgcggcg acctggcaac aaacccgcgc
    13801 cgcgtttgat gccggtgccg acggcctggt gcattgctat aacgggatga caggtttaca
    13861 tcaccgcgaa ccgggaatgg ttggcgcggg attaacggac aagcgcgcct ggctggaact
    13921 gatagccgat ggtcatcatg tgcatccggc ggcaatgtcg ctgtgttgtt gctgtgcgaa
    13981 agagagaatc gtgctgatca ccgacgcgat gcaggcagcc gggatgccgg atggtcgcta
    14041 tacgttatgt ggtgaagaag tgcagatgca cggtggcgtt gtccgtaccg cgtccggtgg
    14101 gctggcgggc agtacgctgt ctgttgatgc ggcagtgcgc aatatggtcg agttgacggg
    14161 cgtaacgcct gcggaagcca ttcatatggc atcgctgcat ccggcgcgaa tgctgggtgt
    14221 tgatggtgtt ctgggatcgc ttaaaccggg caaacgcgcc agcatcgttg cgctggatag
    14281 cgggctgcat gtgcaacaaa tctggattca gggtcaatta gcttcgtttt gatggtttgc
    14341 tcctttattg ggccttcact tcccccgtaa ggcctttctt tttctttcgt tttgatctgt
    14401 gcagcggtgt cggatgcgac gctaacgcgt cttatgcgac ctacggtacg ccgcatccgg
    14461 caatcggtga acatagcgac gtcattacgc aattctttaa tcttcccttg tcttttcttt
    14521 caatgatcac agatttcgtt ttatttcctt tttctccatt gaactttcgg tttcttttct
    14581 atagatttta atcaacgaaa gacatcacca agtgaaatga aacgaaaggc aagtgaaagc
    14641 gacaacgccc gacgtcaagt tcatcagact aaggattgag ttatgccaga aaattacacc
    14701 cctgctgccg ccgcatccgg tacatggact gaagaagaga tccgccatca gcctcgcgca
    14761 tggatccgtt cactcaccaa catcgacgcg ctacgttccg cgctcaataa cttccttgaa
    14821 ccgttactgc gcaaagagaa tctgcggatc atcctgaccg gagccggaac ctcggcattt
    14881 atcggtgaca tcatcgcgcc gtggctcgcc agccataccg gtaaaaactt cagcgccgta
    14941 ccgaccaccg atctggtcac taatccgatg gactacctga acccagccca tccgctgctg
    15001 ttgatctcct tcggtcgatc cggcaacagc ccggaaagcg tcgcagccgt ggaactggca
    15061 aatcaatttg taccggaatg ctatcacctg ccgatcacct gcaacgaagc gggcgctctt
    15121 taccaaaacg cgatcaacag cgataacgcg tttgccgtgc tgatgcccgc agaaacgcac
    15181 gatcgcggct ttgcgatgac cagcagcatt accaccatga tggccagctg cctcgcggtt
    15241 ttcgcacctg agacgatcaa cagccaaacc ttccgcgacg tggcggatcg ttgccaggcg
    15301 atcctgacct cactgggcga tttcagcgaa ggtgtgtttg gttacgcacc gtggaaacgg
    15361 atcgtttatc tcggcagcgg tggcttacag ggcgcagcac gcgagtcggc gctgaaagtg
    15421 ctggaactga cggcgggtaa actggcggcc ttttatgatt ctccaaccgg attccgtcat
    15481 ggaccaaaat cgctggtcga tgacgaaacg ctggtggtgg tatttgtctc cagccaccct
    15541 tacacccgtc agtatgatct tgatctgctg gctgaacttc gccgtgacaa ccaggcaatg
    15601 cgtgtaatcg ccatcgccgc ggaaagcagc gacatcgtcg ctgccggtcc acatatcatc
    15661 ctgccaccgt cacgtcactt tatcgacgtt gagcaggcat tttgcttcct gatgtacgcc
    15721 cagacgtttg cactgatgca gtcgctgcac atgggcaata cgccggatac cccatcagcc
    15781 agtggcaccg ttaaccgcgt ggtgcaaggc gtaatcattc atccgtggca ggcataagag
    15841 gatcgcatta tgagcattat ctccactaaa tatctgttac aggacgccca ggccaatggc
    15901 tacgcggtgc ctgcctttaa cattcataac gccgagacga tccaggcgat cctcgaagtg
    15961 tgcagtgaaa tgcgatcgcc ggtgatcctc gccggaacgc cggggacctt taaacatatc
    16021 gcgctggaag agatctacgc cctgtgcagc gcctattcca caacctacaa catgccactg
    16081 gcgctgcatc tcgaccacca cgaatcgctg gatgatattc gccgtaaagt ccacgcaggt
    16141 gtgcgcagtg caatgatcga cggcagccac ttcccgtttg ccgagaacgt gaagctggtg
    16201 aaatcggttg ttgacttctg ccactcacag gattgcagcg tggaagcaga actgggccgc
    16261 ctgggcggtg ttgaagatga catgagcgtt gacgccgaaa gtgcattcct gaccgatcca
    16321 caagaagcta aacgctttgt cgaactgact ggcgtcgaca gcctggcggt agcgattggt
    16381 acggcgcacg gcttatacag caaaacgccg aagattgatt tccagcggct ggcggaaatt
    16441 cgtgaagtgg tggatgttcc tctggtgcta catggtgcca gcgatgttcc ggatgaattt
    16501 gtccgtcgca ctattgaact tggcgtcaca aaagtgaacg ttgccacaga attaaaaata
    16561 gccttcgccg gcgcggttaa agcctggttt gcggaaaatc cgcagggtaa tgatcctcgt
    16621 tattatatgc gcgtcggaat ggatgcgatg aaagaagttg tcagaaataa aattaatgtc
    16681 tgtggttcag cgaatcgaat ttcagcataa tcattagttt tttatgattt atcccaatgt
    16741 acttcccgat taatatcggg gagtgcctta atggaaaagg agatgactaa accttaataa
    16801 atacatcagt acaatatcgc aacaataata tatttaaaaa cattatatta ttcaacttta
    16861 tggtgaggat tacacaatga gcagtccaaa tattctctta acccgtattg ataaccgtct
    16921 ggttcacggt caggttggcg tgacctggac atccaccatc ggtgcaaatc tgctggtagt
    16981 cgtggatgat gttgtcgcta acgatgatat tcaacagaaa ttaatgggta ttaccgcaga
    17041 aacctacggc tttggcattc gtttctttac tatcgaaaaa accattaacg tcatcggcaa
    17101 agctgcaccg catcagaaga tcttcctgat ttgccgtacg ccacaaacgg tacgtaaatt
    17161 ggtagaaggt ggcattgacc tgaaagatgt caacgtcggc aatatgcatt tctcggaagg
    17221 gaaaaagcaa atcagcagta aagtttatgt cgatgaccag gatctcacgg acttacgttt
    17281 tattaaacaa cgtggcgtga atgttttcat tcaggacgtc cctggcgatc aaaaagaaca
    17341 aatccctgac taaatctaaa atcgccttaa tattggtttg aggtaataaa aatgcatgaa
    17401 ataaccctac ttcagggatt atccctggcg gcgttagttt tcgttctggg gattgatttt
    17461 tggctggaag ccttattttt attccgcccg ataatcgttt gtaccctaac tggcgctatt
    17521 ctcggtgata ttcagactgg cttaattacc ggtggtctga cagagttggc tttcgccgga
    17581 ttaacccctg caggtggtgt tcagccgccc aaccctatta tggcgggtct gatgaccacc
    17641 gtcattgcat ggtctacggg cgttgatgcc aaaacggcaa ttggtcttgg cctaccgttt
    17701 agtttgttaa tgcagtacgt cattctgttc ttctattctg ctttctcatt atttatgacc
    17761 aaagccgata aatgcgcgaa agaagcggat acggcagcgt tttcccgact taactggaca
    17821 acgatgctca tcgtcgcttc agcgtatgcg gtgattgctt tcctctgtac ttacctggca
    17881 caaggggcga tgcaggcgct ggtgaaagcg atgcccgcct ggctgaccca cggctttgaa
    17941 gtggctggcg gtattctgcc tgccgttggt tttggcttgc tgctgcgcgt gatgttcaaa
    18001 gcacaatata tcccttacct gatcgccggc ttcctgtttg tttgctacat ccaggtcagc
    18061 aacctgttgc cggttgccgt actgggcgca ggctttgcgg tgtatgagtt tttcaatgcg
    18121 aaatcccggc agcaagcgca accgcagccc gttgccagta aaaatgaaga agaggactac
    18181 agcaatggga tctgaaatca gtaaaaaaga tatcacccgt ctgggctttc gttcgtcgct
    18241 gctgcaagcg agctttaact acgaaaggat gcaggcgggc ggttttacct gggcgatgtt
    18301 gccgatcctg aaaaagattt ataaggatga caaaccgggc ctaagcgcag cgatgaaaga
    18361 taacctcgaa tttattaata cccacccgaa tctggtcgga ttcctgatgg ggttattaat
    18421 ttcgatggaa gaaaaaggag aaaaccgcga caccattaaa ggcctcaaag tggcactgtt
    18481 tggcccaatc gccgggattg gcgatgcgat tttctggttt actttgttgc cgattatggc
    18541 gggaatttgc tcatcatttg ccagccaggg aaacctgctg gggccgattc tatttttcgc
    18601 cgtttacctg cttatctttt tcctgcgcgt cggctggacc cacgtcggtt attcagtcgg
    18661 cgtgaaggcg atcgataaag tgcgagagaa ctcgcagatg attgctcgct cggcaaccat
    18721 cctcgggatc acggtaatcg gcgggctgat cgcttcgtat gtgcatatta acgtggtgac
    18781 atcgtttgcc atcgacagta cccacagcgt cgcgctgcaa caggatttct tcgataaagt
    18841 cttcccgaac attttaccga tggcctacac cctgctgatg tattacttcc tgcgggtgaa
    18901 aaaagcgcat ccggtgctgt taatcggcgt gacttttgtg ctctctattg tttgttccgc
    18961 attcggcatt ttgtaaatgg aacgaggcac tgcgtctggc ggtgcctctt tacttaagga
    19021 atttcatcct gtgcaaaccc ttcagcaagt tgaaaactat acggcgttaa gtgaacgtgc
    19081 cagcgaatat ttattggccg tgatccgtag caaaccggat gccgtgattt gcctggcgac
    19141 cggagccacg ccattactga cgtatcatta tctggtagaa aaaatccacc agcagcacgt
    19201 tgatgtcagc cagctcaccg tcgtcaaact cgatgaatgg gtggatctgc cattaacgat
    19261 gcctggtacc tgcgaaactt tcctgcaaca gcatatcgtg cagccgctgg ggctacgtga
    19321 agaccagctc atcagctttc gctccgaaga gataaatgag acagagtgcg aacgggtaac
    19381 gaacctgatt gcgcgcaaag gcggtctgga tttatgcgtt ctcggattgg ggaaaaacgg
    19441 tcatcttggg ctgaacgaac cgggggaaag cctacaaccg gcctgccata tcagtcaact
    19501 tgatgccaga acacagcaac atgagatgtt aaaaaccgcg ggtcgccccg tgactcgtgg
    19561 gatcacctta ggcctgaagg atattctcaa tgcccgcgag gttttgttac tggtgactgg
    19621 cgaaggaaag caggatgcga cagaacgttt tctcacggct aaagtctcta cctctatccc
    19681 ggcttcgttt ttgtggctgc acagtaactt tatttgttta attaatacct aaccagatga
    19741 aaaatttgct caaaatgttt aaattaactt attcaacagt cacgcattat attaaataac
    19801 atttgactgg gttgaacata acgccaatag caaaaggagt tatgttcaac aatttaattt
    19861 tagatataca tataacttgt tctattaata aaccatctcg tattctgact cgccccctgg
    19921 cgcagcaaag tc
//
LOCUS       DAZFKF010000094        19517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFKF010000094 DAZFKF010000000
VERSION     DAZFKF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.094"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..322)
                     /locus_tag="ACHKA1_003922"
     CDS             complement(<1..322)
                     /locus_tag="ACHKA1_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5681506.1"
                     /translation="MAQLRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCICGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPE"
     gene            364..564
                     /locus_tag="ACHKA1_003923"
     CDS             364..564
                     /locus_tag="ACHKA1_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5681507.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMKNNEK"
     gene            complement(696..1022)
                     /locus_tag="ACHKA1_003924"
     CDS             complement(696..1022)
                     /locus_tag="ACHKA1_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO5681508.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(1367..1591)
                     /locus_tag="ACHKA1_003925"
     CDS             complement(1367..1591)
                     /locus_tag="ACHKA1_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681509.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQCGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            complement(1677..1862)
                     /locus_tag="ACHKA1_003926"
     CDS             complement(1677..1862)
                     /locus_tag="ACHKA1_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO5681510.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISPSESG"
     gene            complement(2084..2170)
                     /locus_tag="ACHKA1_003927"
     CDS             complement(2084..2170)
                     /locus_tag="ACHKA1_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681511.1"
                     /translation="MSAELIRAMAFAIRIVAIAVLVWAIRWW"
     gene            complement(2182..2337)
                     /locus_tag="ACHKA1_003928"
     CDS             complement(2182..2337)
                     /locus_tag="ACHKA1_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681512.1"
                     /translation="MGDVVFVGAAGVTVTDDISRALAFAIKWVAVGIAVSPMLYGLAK
                     LVIALKS"
     gene            complement(2301..2432)
                     /locus_tag="ACHKA1_003929"
     CDS             complement(2301..2432)
                     /locus_tag="ACHKA1_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681513.1"
                     /translation="MSDKLITPAKVLCVIVGISFSLMLVALFLSLAWVMLSSSGLLG"
     gene            complement(2725..3258)
                     /locus_tag="ACHKA1_003930"
     CDS             complement(2725..3258)
                     /locus_tag="ACHKA1_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5681514.1"
                     /translation="MNAKIRYGLSAAILALITAGASAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATRVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            complement(3681..4022)
                     /locus_tag="ACHKA1_003931"
                     /pseudo
     CDS             complement(3681..4022)
                     /locus_tag="ACHKA1_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
     gene            complement(4027..4242)
                     /locus_tag="ACHKA1_003932"
     CDS             complement(4027..4242)
                     /locus_tag="ACHKA1_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5681515.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(4318..4608)
                     /locus_tag="ACHKA1_003933"
     CDS             complement(4318..4608)
                     /locus_tag="ACHKA1_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5681516.1"
                     /translation="MSEIKSLVTAEAVKEVLRSEEVRSALKQQLRQNLEARLDAEVDS
                     ILDELLGAQPEPSPELLPEPQAEDVTTENGDIQPESPVTDMTDTQPEPGTML"
     gene            complement(4634..4828)
                     /locus_tag="ACHKA1_003934"
     CDS             complement(4634..4828)
                     /locus_tag="ACHKA1_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5681517.1"
                     /translation="MTFVHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGAKTEAPLIPEQPS"
     gene            complement(4968..6950)
                     /locus_tag="ACHKA1_003935"
     CDS             complement(4968..6950)
                     /locus_tag="ACHKA1_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5681518.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVQPSGDGGAVISTTSEPEYYFVIALAGQSNSMSFGEGLPLPDTY
                     DRPDPRIKQLARRSTVTPGGAACAYNDIIPADHCLHDVQDVSNLNHPKADLNKGQYGC
                     VGHALHVAKKLLPFMPARAGILLVPCGRGDSGFTAGAEGAFNEASGATAGSSLWGVDK
                     PLYRDLVSRTRAALKKNPKNVLLSVIWMQGEKDVSSGRHAEHNALFLAMVNKYRADLA
                     DIADQCIGGTTSGVPWICGDTTYDWKAKYAVQYEAVYGGYKGKAAQNIHFVPLMTDEH
                     GANVPTNEPSEDPDIITAGYYGAASRSNGNWTTADRKTHFSSWARRGIVSDRLAGEIL
                     VRAGRLLPFLSGQSAPLATTPASTGDAQSGSAGPTQQQGAGTSAGGHTEAVTRMVAGY
                     DANSGSGVWTEQQWNASGGKGTVTDDSGRKALRLEKQPGKLTSWKMFRTVAVEEAKNL
                     LSKGGEIAVRFKIPEGVELVNGQFVFGLYWPVSQWASGATANSMLASFFLQTDASNLN
                     LMYHKGESNAQLGTFGAFDHNWHTVVFRFAGNNSEKVVPVIDDAEQSAFDLVMWTNDG
                     FTADTLTLTDITGAKATYPILLDTVTVKVNESRASS"
     gene            complement(7724..7800)
                     /locus_tag="ACHKA1_003936"
     tRNA            complement(7724..7800)
                     /locus_tag="ACHKA1_003936"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7764..7766),aa:Arg,seq:tct)
     gene            complement(7814..7890)
                     /locus_tag="ACHKA1_003937"
     tRNA            complement(7814..7890)
                     /locus_tag="ACHKA1_003937"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7854..7856),aa:Arg,seq:tcg)
     gene            complement(7898..7973)
                     /locus_tag="ACHKA1_003938"
     tRNA            complement(7898..7973)
                     /locus_tag="ACHKA1_003938"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7938..7940),aa:Ile,seq:cat)
     gene            complement(8014..9072)
                     /locus_tag="ACHKA1_003939"
     CDS             complement(8014..9072)
                     /locus_tag="ACHKA1_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO5681519.1"
                     /translation="MLSTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPDESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKLLRRYFGVTAQVSYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLASQNG"
     gene            complement(9223..9420)
                     /locus_tag="ACHKA1_003940"
     CDS             complement(9223..9420)
                     /locus_tag="ACHKA1_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO5681520.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(9633..10322)
                     /locus_tag="ACHKA1_003941"
     CDS             complement(9633..10322)
                     /locus_tag="ACHKA1_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO5681521.1"
                     /translation="MNNQYLQFVREQLMIATADLSGSTKCQLEAWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIQGKQSLAKGSSIALVSPVEFATSSWRRAVLELEEHQK
                     AWLLWCYGGNICWEHQIAITQWVWSEFKTQSGSRKIAVKTLERVKKLIWLAAQDVRGW
                     VTGCEVYQRQELARLCGVKPDNWSHNYANYWREMCDIFKRLDRESLICSVKIRAQQKA
                     TFSRRDIAKVN"
     gene            complement(10315..10677)
                     /locus_tag="ACHKA1_003942"
     CDS             complement(10315..10677)
                     /locus_tag="ACHKA1_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5681522.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVVLIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHVGLLMDDEQFDEINIVRGQ
                     PVSGGRLEIRITEVGVHE"
     gene            complement(10690..11736)
                     /locus_tag="ACHKA1_003943"
     CDS             complement(10690..11736)
                     /locus_tag="ACHKA1_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5681523.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAACNQVADALPEAVLRRSLGLRAEKIRPVYRESDIIPGEPTATSILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPESLMKRPKRRRWVNEKYTRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEKKYGSQIELI
                     FRFLDHAFATGVLG"
     gene            complement(11738..12016)
                     /locus_tag="ACHKA1_003944"
     CDS             complement(11738..12016)
                     /locus_tag="ACHKA1_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681524.1"
                     /translation="MAHDTKLYNSDNSAVFASRHGRRSHAFKSDRLQHAPCTEEQAEW
                     LIQNYRRRGYEFRKTLSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(12184..12339)
                     /gene="hokD"
                     /locus_tag="ACHKA1_003945"
     CDS             complement(12184..12339)
                     /gene="hokD"
                     /locus_tag="ACHKA1_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO5681525.1"
                     /translation="MKQQKAMLVALIVICITVIVTALVTRKDLCEVRIRTGQTEVTVF
                     TAYEPEE"
     gene            complement(12585..12689)
                     /locus_tag="ACHKA1_003946"
     CDS             complement(12585..12689)
                     /locus_tag="ACHKA1_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681526.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
     gene            complement(12805..13605)
                     /locus_tag="ACHKA1_003947"
     CDS             complement(12805..13605)
                     /locus_tag="ACHKA1_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dATP/dGTP pyrophosphohydrolase domain-containing
                     protein"
                     /protein_id="HFO5681527.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEREQIRH
                     EHAKWSDSTFGCVGPIGPLKHLSKEALEAAAEPDDLSEWADMQFLLWDAQRRAGISDA
                     EITAAMEDKLKINMERQWPEPKDGEPRLHIKEPGNSPVIPDGLSTVCAEAYQVVGVMA
                     DALGVFGDAAVQKVLDNLSQQKLVHRDVLPFSLPVTPDGWISCSERMPDDGQHVIILC
                     DGAFVLYAQYRDGEFFDVVRNGDEFFETQSRNVTDWMPLPEPPQEVRQ"
     gene            complement(13608..13799)
                     /locus_tag="ACHKA1_003948"
     CDS             complement(13608..13799)
                     /locus_tag="ACHKA1_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681528.1"
                     /translation="MNGQISIVRPGACDDREIRMIIRLAMGRTITALITPENLALALT
                     GKSELPVELKLRNVEIKVK"
     gene            complement(13897..14349)
                     /locus_tag="ACHKA1_003949"
                     /pseudo
     CDS             complement(13897..14349)
                     /locus_tag="ACHKA1_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            complement(14366..14586)
                     /locus_tag="ACHKA1_003950"
                     /pseudo
     CDS             complement(14366..14586)
                     /locus_tag="ACHKA1_003950"
                     /inference="COORDINATES: protein motif:HMM:NF027274.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
     gene            complement(14606..14908)
                     /locus_tag="ACHKA1_003951"
     CDS             complement(14606..14908)
                     /locus_tag="ACHKA1_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO5681529.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHRAEDALKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMLRCVDAVYMLKGWQQSAGARAELALAEKLGHAVIFQEVGSEY"
     gene            complement(14905..15222)
                     /locus_tag="ACHKA1_003952"
     CDS             complement(14905..15222)
                     /locus_tag="ACHKA1_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5681530.1"
                     /translation="MSGWKKWRWAEILILRQCAGTMRVESIGYLIGRSESAVRTKARE
                     LGISMILRGDFHPSAKYSQRDIELARQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRR
                     VQE"
     gene            complement(15219..15935)
                     /locus_tag="ACHKA1_003953"
     CDS             complement(15219..15935)
                     /locus_tag="ACHKA1_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5681531.1"
                     /translation="METVFDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVQEMM
                     AQEEMKTEIVEDIVRLQPSITEMKADDLILPSLHVANRELRRAKSDVQKWERVCAALR
                     ELNKHRDILRDITATREQQR"
     gene            complement(15969..16430)
                     /locus_tag="ACHKA1_003954"
     CDS             complement(15969..16430)
                     /locus_tag="ACHKA1_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5681532.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            complement(16423..17460)
                     /locus_tag="ACHKA1_003955"
     CDS             complement(16423..17460)
                     /locus_tag="ACHKA1_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO5681533.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKYDADCHDESRRVTHLSRQVTTDKDTDTE
                     LNPTHNARMRESAPTGESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSSDFRQRAA
                     VWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRTQVKPVSKGVS
                     HAASGGTASRAVQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDPEERGRTF
                     EALDCPDWRDD"
     gene            complement(17529..17954)
                     /locus_tag="ACHKA1_003956"
     CDS             complement(17529..17954)
                     /locus_tag="ACHKA1_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5681534.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAVAIAWGTLTNSGGQPGNAVVVH"
     gene            complement(17938..18210)
                     /locus_tag="ACHKA1_003957"
     CDS             complement(17938..18210)
                     /locus_tag="ACHKA1_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5681535.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFDVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGFKVNTSNAPELIHENQA"
     gene            18320..18721
                     /locus_tag="ACHKA1_003958"
     CDS             18320..18721
                     /locus_tag="ACHKA1_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5681536.1"
                     /translation="MMNALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVTGHPLHWFLLPPEEGEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            18749..18940
                     /locus_tag="ACHKA1_003959"
     CDS             18749..18940
                     /locus_tag="ACHKA1_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO5681537.1"
                     /translation="MSTALSPIVSEFETTEQENSYNEWLRAKVASSLADPRPSIPHDE
                     VMAEMENLIAQIAVTNRSE"
     gene            18940..19227
                     /locus_tag="ACHKA1_003960"
     CDS             18940..19227
                     /locus_tag="ACHKA1_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5681538.1"
                     /translation="MLPILWLPSARDDFRQIVAYIAKENIPAARRLKIRIETSVLALS
                     EHPYLYPPSDRVSGLREIVVHPNYIVLYRVAASSIEIANIVHARRQFPFPI"
BASE COUNT         4678 a         5511 c         4370 g         4958 t
ORIGIN      
        1 cctccggcag cgtcgggtcc ggcataccac ccgccgcttc acgagcagac ttttgtcgat
       61 ggcattcggt acagagcgtc cagagattcg tctcctcatt accaccaccg aactgaagtg
      121 caattcggtg atcgagttca ctgtcacaga ggtcaaccac acgaccacag atacagcact
      181 gcccggcgtc cctcagccag atatgacgct tgagggaaac acgtgcactg ccactgacac
      241 gacgctgctc ccccttcaga atattcaccc gtcgggtatt cagtgttttg attctgctct
      301 ggagtgtacg aagctgagcc atgtaaaatc cccgtcatat ggcaatcagt aaaggaaata
      361 aatatgtcat cgaaaaaccg gacccgcaga accacaaccc gcaatatccg tttccccaat
      421 cacatgattg aacagatcaa catcgccctt gagcataaag gatccggtaa cttttcagcg
      481 tgggttattg aagcctgcag gagaaggctg gcaacagatg caacgcatct gcgcccggcc
      541 agcatgaaaa ataacgagaa atgaacgttc ggttacagga gcaggtaccc actgtcctcc
      601 aacaatattt catcttcata tccgacggaa caagacttac cctgccggga tgtacagaat
      661 aacaacagag tgataattaa tttctgatga aataatcagg gtgcagaagg actaaagata
      721 aacgttttct tcacgccttt acacggcctg tccttctcaa atcgccattt tgccatcgcc
      781 tttacaacct gctcatcaaa caaatggtgc ggctctgaac ggataaactc aattcgggtg
      841 acagtaccat cagcaccaat atcaaacttc acatcaaccc gtccctttat ataatttgcc
      901 gctgcatagg ccggatattg tggtaatgcc ttaaccaact gtcggggcat atctgtttta
      961 tgttgcgtac agcccataac cagagaagac aacaaaataa ttaacggaag atttcttttc
     1021 attttcattc ccggcacaga taagaataag tcttattcta acaatgccac cctgtcggtc
     1081 atcaatcctc tgtttaatgg caacgacaat tatccgactt aaatcacaaa tcagacacat
     1141 gacataacag agcttgcgag gtaacacatc gtccggtttc ttccaccatc gcaccggacc
     1201 agcgaccatg aggggacaac gccgcgctcc gttaacgcgg taaaccccgg tgtgtatcgt
     1261 ttttgattat ccccgcacac tcgcgcagag gagtctcccg gtcgggctgc ggtctctgtt
     1321 aatgcaggaa tacggcgaca ataccgcgca tggataataa ggtcgctcaa cacactggct
     1381 gtaatgcagc ggataccatg cggcatttag cggcattcat cgtacactca acggttagct
     1441 cttcattcgt ggcattcacc tgaaaggtcc gggagtgtaa ttgcgtacat ttaccactga
     1501 acgaaccttc aacaagaaca cgaccacact gcaaaatacg gaacggaatt gttccctgaa
     1561 aaggctttac ggttaccagt aatttcttca tgcattctcc ggataacaaa aatactagtt
     1621 aatacactga gtgcggatat agtcctgcgc cccttccagt tgcttctgca ttgtcatcaa
     1681 ccgctctctg agggtgaaat aatcccgttc agcggtgtct gccagtcggg ggccggttgc
     1741 attatccatg ccggaggtgc cggtggcttc acgcacggta ccggagcagg tggcgttgat
     1801 ccgcaggcgc ttacgaccag cggcaacatc agcacgcaga gtttcatttt cagctctcgc
     1861 atcggctaat tccctcgagt atctggcatc aagtgcagca acatcacgct ggcgctgctg
     1921 catatcagta atggttgcat ttgcctgctc cagctcactg acttttttat cgcgctgctc
     1981 tttgtaggtt atggcgttat cacggtaatg attcagcccc agactaagca caccacaggc
     2041 taccagcatg acaataatca ccacacacag aacacggttc atatcaccac caacgaattg
     2101 cccagaccag aacagcaatg gccacaatac gaatggcaaa tgccattgcc cgaataagtt
     2161 cagcactcat ctttttaaag ttcacgattt cagcgcaatg accagttttg ccagcccata
     2221 cagcatcgga gacacagcaa taccaacagc cacccactta atagcaaaag ccagcgctct
     2281 gctgatgtca tcagtcactg tcaccccagc agccccgacg aagacaacat cacccaggcg
     2341 agggacagaa aaagagcaac cagcattagt gaaaatgaaa taccgacaat cacacacagg
     2401 acctttgccg gcgttatgag tttgtctgac atagctaccc cttaattgcc acaattaact
     2461 gggatactac ccataaaaaa gggatgctcc agaccagcaa aaatttccag tttggtaatt
     2521 gactaatcat gagtcgcaac tccctaatca gtttgctaaa atcaatcaag gcagcctccc
     2581 atagcttact gccataaaaa caaaaccccg cttgctgcca acaaacgggg tttttacttt
     2641 tattcactta cattttgcca gttcgcagga tttcgtgtta tccgtccgcc ttggccaacg
     2701 tcatttatta gcaaaatatt ctgcttatct gtcgattccc cagcacgcca gcgcgctctc
     2761 ctggtctcgc cgggatacct gaccgtaaca gttatttgag cgaatacggc agtccctgcc
     2821 accgtcctta atccaccagc gaatcgcttc gcaggcacct tttcgatcac cagcattaat
     2881 tcgtctgtaa aacgtcgacg ggaaacactt accgggacca atgttgtacg gacagaatga
     2941 cgcgatcccc gctttctggg gttcggtcag cggcactctg atgtttttct ccacccacgc
     3001 cagcgcttta tcacgctcaa tggcgttaac ctggtcgcat ttttccttcg acaacttcat
     3061 gccggggacg acaggcttac catccacccg ggtggcacca cggcagatgg tccatatacc
     3121 agaaccatca cggtatgctg tggtgtggtt accttctttt tcatccagaa actggtcgag
     3181 aatgtcaggc gcagatgcgc ctgcagtaat cagcgccaga atggcagccg acaggccgta
     3241 tctgattttt gcgttcatgg atatttatca ggatttatcg gttccgaatc cctggatatg
     3301 ttaagtcttc agcccgccag tggtgggcac tggcgtaaac tcaacaatgc gagatggctg
     3361 cctcgcaaag ataatcaaag caatggatgc actaagtaaa aataacgctg tgtcagatta
     3421 cctgatcgcc gaaatggaac atctaattaa agaaatacca aagacagttg ctcgtgatga
     3481 taaaagattc ctcaaggctg ctgaagatgc tctttcaaca gtgaagcgca gcttagatgc
     3541 gccgcgcgat caagaatagt ttctttcatt tcatctgctg caagcacctc attttttgtt
     3601 gggatgcttt ttttcaattc agcgatatag cactccagtt tttcaatgcg tgattcaata
     3661 tcatcgtttt ctgaccgcaa tgccgtcggt ggcgtcttca gagaaccagt aattattccc
     3721 ggtagttttc ctctgtaggt tatcaacaca tcctgcgcct ctaaaattat ggggcgcttt
     3781 tccggcgact gctcatcccc ttcacataaa gcggcagcaa tatccatgaa aaactgcttc
     3841 gcctgttttt tcgcctcagc ttcgtaaaac tccagcgtgg caccttcagt acggtcaaga
     3901 ctaatcgcca catatggcaa caacagtgac ggatacccac caatttccag tgccacagta
     3961 gcagtaatct tatccgggta attatttatc cctttaacaa ccagtttgta ttgtttattc
     4021 atcgccttac tctccccgcg ctgccttacg acggtcctct ctgattttga aatacaggtt
     4081 agtcagatat gtcagcagcc caaacagcag actccccagc acgcctattg ccgcccactg
     4141 agacggggaa accctgtcca gcaactgcag gaaccagtag cccgttccca ccgctgacgt
     4201 ggtgtatgac acacctgttg tgattttttc catctggtac ataccccgtc tcccgtactc
     4261 ggaagctcac aacaacaaaa gggccaccag ctctttactg atggccctgc cccgccgtta
     4321 cagcattgtg cccggttcgg gttgtgtgtc tgtcatatcc gtcaccggtg actccggctg
     4381 aatatcacca ttttccgtgg tgacatcttc cgcctgtggt tccggaagca gttccgggga
     4441 tggttccggc tgtgcaccaa gcaattcatc cagaattgaa tccacttctg catcaagacg
     4501 cgcctcaagg ttctgccgaa gttgctgttt cagtgcgctc cggacttctt cagagcgcag
     4561 gacttccttc actgcctcag cagtgaccag cgattttatt tctgacatag gattttctcg
     4621 ttgaaagggg ttattaagaa ggttgttccg gaatgagtgg ggcttctgtt tttgctccgg
     4681 ctgactgact ggcgctgatt ttctctgcgg cccttttatc aatctgcctg cgccagaaat
     4741 cgcgcactgc cctgtaccca cccgaaagaa gatacagcac acagaccgcc gtacagaagt
     4801 acagcatcac ctgatgaaca aatgtcatag tttcttaccg ttatggttga caatgagaac
     4861 tgttttcatt taaaaaaccg atatacgaaa gcatcttttc tttacattct ccattgggat
     4921 tacctccgcc agcttccgtt cctgccgctg gcggtttttt ttgccggtta tgatgatgcc
     4981 cggctttcgt taactttgac tgtgaccgta tcaagcagta tcggatacgt cgcttttgcc
     5041 cccgtgatat ctgtcagcgt cagcgtatct gctgtaaagc catcatttgt ccacatcacc
     5101 aggtcaaacg cagactgctc tgcatcatca atcaccggca ccactttttc gctgttattt
     5161 cccgcaaagc ggaaaacaac tgtatgccag ttatggtcaa atgccccaaa tgtgcccagt
     5221 tgcgcattcg actccccctt gtggtacatc agattcagat ttgatgcatc cgtctgaagg
     5281 aagaaggacg ccagcatgct gtttgctgtc gcgcctgacg cccactgcga caccggccag
     5341 tacagaccaa agacaaactg accgttaacc agttcgacac cctccgggat cttaaaccgc
     5401 acggcaattt cacctccctt actgagaaga ttttttgcct cctccaccgc aacagtacgg
     5461 aacatcttcc aggaggtcag tttacccggc tgtttttcca gtcgcagcgc cttcctgcca
     5521 ctgtcatccg tcacagtgcc tttaccaccg gacgcattcc actgctgctc tgtccataca
     5581 ccactgccac tgttcgcatc atatccggcc accattcttg ttacggcttc agtatgaccg
     5641 cctgcagaag tacctgcacc ctgctgttgc gtcggacccg cagagccaga ctgtgcatcc
     5701 cccgtggagg ctggcgtggt cgccagcggt gcagactgcc cgctcaggaa cggcagcaaa
     5761 cgcccggctc gcaccagtat ctctcctgcc agccgatctg aaacaatgcc tcttcgcgcc
     5821 caggagctga agtgcgtttt acgatcggct gtcgtccagt taccattact gcgtgacgcg
     5881 gcaccatagt atcccgccgt gataatgtcc ggatcttctg acggctcgtt tgtcggcaca
     5941 ttcgcaccat gctcatccgt catcaacggc acaaagtgaa tattctgcgc cgccttgcct
     6001 ttatagcctc cgtaaaccgc ctcatactgc actgcatact tcgccttcca gtcgtacgtg
     6061 gtgtcaccgc aaatccacgg gacgccggac gttgtcccgc caatacactg gtctgcaata
     6121 tctgccaggt ctgcacggta tttatttacc atggcaagaa aaagtgcatt gtgctctgca
     6181 tgtctccccg aactgacatc tttttccccc tgcatccaga tcaccgacaa cagcacgttt
     6241 ttcggatttt tcttcagggc tgcacgcgtt ctgctgacca ggtcacgata caacggttta
     6301 tccacccccc acagggaaga gcccgctgtc gcacccgacg cctcattaaa cgcgccctct
     6361 gctcccgcag taaatcccga atcgccacgt ccacacggga caagaaggat ccccgcacgg
     6421 gcaggcataa acggcagcag ttttttggcc acatgcaggg catgccccac acagccatac
     6481 tgccctttat tgaggtctgc tttcgggtga ttcagattac tcacgtcctg cacatcatgc
     6541 agacaatggt ctgcaggaat aatgtcgtta tatgcacagg ccgcaccgcc cggtgtcacc
     6601 gtgctgcgac gcgccagttg cttaatacgc gggtcaggac gatcatacgt atccggcagc
     6661 ggcagcccct cgccaaaaga catcgagttg gactgcccgg ccagcgcaat gacaaagtaa
     6721 tattccggtt cgctggtggt gctgataaca gcgccaccat cacccgacgg ttgcaccacc
     6781 accggtgtgg tgacatcacc ttccgccgca atggcctgca tcagggtata gggcgtgatg
     6841 gcgacagggc tgccaaatgg ctgccacccc tccttcagtt tttgtgtcag tcgctccgca
     6901 aggtctgacg gcgacgccgc cctgaccaca tcgtagtgtt taaatgtcat gaatcctccc
     6961 ggtcgggata atgttgtgag tcagataagg agcgagctga agtccggaag ttacaggaca
     7021 atggcagaag ggagactaca gcccgcaata cgaaaaaggc cgcgcagttg cgcagagtga
     7081 ttactatggg gtattattcg ccagctgaaa tattacttca cgttttattg tttattcctt
     7141 gccgcccgcg tctcccagcg cgggcttttt tgtccataag aaagcccctc cggagagggg
     7201 ctgaagccgc atttctgtat caccatgaac atggtgccgg gtgcctcccg gtgagttcag
     7261 cccggtgcca ctgaacccgc gatctcttct gggtcatcac ggatgatgaa ttgttgtcac
     7321 cagtcgcccc accgcacagg gggattcacc atgcaatact tttttaacaa aaaagataaa
     7381 acgaaatcaa cactcaactc actgaaatgt gaagaattta acattttcac ataagaacgt
     7441 atgttccttg tcacatcccc gatcagaaca caaatcagcc tggcaaacta aatctgcgga
     7501 ttgtgaccat cgtaatggag ccttgccagt cgccccaccg cttaggggga ttcatcatgc
     7561 ggagtgattt ttaacaaatt ccccaccaga aaaacaataa tcaccttcct gaattgtgag
     7621 gtatttaaaa atttcaccag ataactgata cccgactaac catctgatgt tttctttttc
     7681 agcaaattaa aaggcccgcc gaagcgagcc aggaaaataa gtgtggcgcg ttgtactgga
     7741 ttcgaaccag tgaccgatta cttagaaggc aattgctctg tccggctgag ctaacaacgc
     7801 agaataccga taatggaccg ccatcgagga ctcgaacccc gcgcaaccag cttcgaaggc
     7861 tggcgctctt tcctgatgag ctaatggcgg tatgtgatgg tggcccttgc tggatttgaa
     7921 ccagcgacct ggcgattatg agtcgctcgc tctcaccact gagctaaagg gccgggcgca
     7981 ggataataac ggtacgtaac taatcctgca atatcatccg ttctgactgg ctaaatcctg
     8041 aacttccctg accgtctgct caaaacgttc agtctccagc tcaacgcctg tagcacgacg
     8101 ccccagcgcc atcgcggctt tgactgtcga acccgacccc atgaagaaat ctgcaaccag
     8161 gtcacccgga cgactgctcg cactgattat ctgctgcagc atttctgccg gtttttcgca
     8221 cggatgtttc ccgggatagt actgcaccgg tttatgcgtc cacacatccg tgtacgacac
     8281 ctgcgccgtc acgccaaaat accgccgcag aagcttatat tcactctgca gttccgcata
     8341 ctgccggttc agtgaagtat acgtctccag cagctggtgg tggggctttt ccagttcacc
     8401 ccgctgatgc ttctcttctg ccacccgggc aaacagcgcc tgtaatttca gataatcact
     8461 ttcgtccggt agctgccact gactggcact gaaccagtgc gacaccatgt ttttctttcc
     8521 tgtggcatca gcaatctgtt ttgccgttat ccccagggca gcacgcgcat cacgaaagta
     8581 agaaatcagc ggggccatca catgctgttt cagtgccctg cccttcgcct catacccggc
     8641 atctttcggg cgatacggcc cctgataatg ttccgcgaac agaatgcgct ctgtggcggg
     8701 gaaatatgcc ctcaggcttt ccttgttgca cccgttccag cgtccggacg gcttcgccca
     8761 gataatatgg ttcagcacac tgaagcgttc acgcatcatg atttcaatgt cagacgccag
     8821 gcgatggcca cagaacaggt aaagacttcc ggcaggtttc agcacccgcc agaactgcgc
     8881 aagacactgg tccagccact tcaggtaatc atcgtcgccc ttccactggt tatcccagcc
     8941 ctcaggcttc actttaaagt acggcgggtc cgtgactatc aggtcaacag aattttcggg
     9001 taacgaccgg ataaattcca ggcagtcggc gttgattaac tcacaactgg atatttttac
     9061 agtactaagc atggatcatt aagcctgtct ctgataggct cattctgctt ttgcgcaaag
     9121 cagtgggcct gaggtttgct tgtgatccgg acgcatgagc agatggctgg tgagtgcccc
     9181 taacacccac cagccgccca tttaccacaa ataaaaaagc cttcaggact gaaggcgtct
     9241 gtaacaaccg aactgatagt ctgccagacc cgccataaca agctgggtca gtattaactg
     9301 acagcgttcg cgtgaaaggt acgtattctg cgcaatctcc ccgactgtcg ccggttcggt
     9361 gacgcttaat tcattaaaca ccactctggc ggtttcggtc atatcctgct gtttcagcat
     9421 gtctttttcc cttttccggt taacgtgaca caccaataac tcttgtcgaa aaagccagca
     9481 agctgaaaga caggtattca ccgccaccag cgcgtttact gtactgacgc gattttcgga
     9541 cataaaaaaa accacctggc ggtggttttt tcttactttg ccatcacgta caaaatcggc
     9601 aaaatatcag atttatacga aacatacgcg atttaattga cttttgcaat atctcgtcgt
     9661 gaaaaggtcg ctttttgttg cgctcttatt ttcacggagc aaatcaagga ttctctatcg
     9721 aggcgcttaa aaatatcgca catctcacgc cagtagttcg cataattatg gctccagtta
     9781 tcaggcttaa ctccacacag tctggcaagc tcctgtctct ggtagacctc acacccggta
     9841 acccatcctc tgacatcctg tgccgccagc cagatcaact tcttcacacg ctccagcgtt
     9901 ttcactgcaa tttttctgga gccggactga gttttaaatt cactccacac ccactgcgtt
     9961 atcgcgatct gatgctccca gcaaatgttt ccgccataac accacaacaa ccacgccttc
    10021 tgatgttctt ccagttccag aacggcacgc cgccacgatg atgttgcaaa ctcaacagga
    10081 ctgaccagcg caattgatga gcctttcgcc agtgattgtt taccctggat cgggggatta
    10141 tcccgcgtga tcatttttcc ggttacctca tcgcggtaac gaattttttt gcgtctgtaa
    10201 cgccctgtat cgaacagggc attttcctgc caggcttcca gctggcattt tgtcgaccca
    10261 ctgagatctg cagtggcaat catgagttgc tcacgaacaa actgtaaata ctggttattc
    10321 atgcacaccc acctctgtaa ttcttatctc cagccgtcca ccagatactg gctggccacg
    10381 tacaatattg atttcatcaa actgctcatc gtccattaac aaccccacgt gcgtcagcgc
    10441 atccagtggt gctttcagaa tattgtccag gtcacgacga cgcttatccg gtggctctgc
    10501 aataattttt attgccagcc gtccggacag gcttaatttc agtcgctgct ggcgaacaat
    10561 aagcaccact gcccggcgat aacgctcccc ggcttttgat acaaaatatg tgctgccacg
    10621 gcgtcgccag taagtgttca ccgtcggcgg gtaaggtaaa accaaatcta tgagcatcag
    10681 tcacctcttt tacccgagca cgccagtcgc aaaggcgtga tcaagaaaac gaaaaattaa
    10741 ctcaatctga gagccgtact ttttctcaaa ctccagcggg tctgcatgaa gttcgttgtg
    10801 gtgctcccgg cacaacggta gcgtgaaaat atcgtgggcc tttgtcccca ttcccccctg
    10861 accatgacca atcaggtgat gcggatcgtc agcaggctta ccacaacacg cacacggctg
    10921 tgtctttacc cagcgcgtat atttctcatt tacccaacgg cgacgtttag gtcgcttcat
    10981 taaagattcc ggagactccg gatcaacagc aatgctgacc accgtctttt cctgtggcgg
    11041 gttttgctgg tgggcgtgag gcagcggcgc aagatttttt gtgcgctgct tcagtatgct
    11101 ggtggcggtc ggttctcccg gtatgatgtc gctttcgcgg tacaccgggc ggattttttc
    11161 cgcacgtaat cccagcgaac gacgcaatac tgcctccggt agcgcgtccg ccacctgatt
    11221 gcaggcagcc caccaggata attcagccag cgataattcc cgctcctgcg tgccattcat
    11281 tgcatggcgt atgacgtcaa tcatccatgc tgacaggttt tggtgagcaa gttgcccgag
    11341 tgattcggaa gtctggtcac gcagctggtt gtcgcagtgc cagcacaaca ccatcgcgcc
    11401 ggtaccgtaa cgatgtatga cggtttcact gtgatgataa tcaccgtgtg gccactggca
    11461 ggatttcacg tgacgtaata accagtcaga cagtgcacca gcgccacccg cagcacgaat
    11521 cacccgttcg ttgctgaaaa atggcagtaa tgttttatcc tctgccagcg gctgtcgaac
    11581 ggcaggaacg actccggacg gcagattacg catgcttttc ggttccggct ccaccagtac
    11641 ccgggtattg tggaataccg gcatggattc acggcccggc ttaacgatca ccagcccgag
    11701 ttccggtacc agaacaggtc gaagtaatac ccgcacgtta cctccagatg cgttgctgga
    11761 atgtgcggga cggacgcggt gggcgttcag agtaaggaag cctgacggag attatccagt
    11821 gacgataatc gaggctgagg gttttcctaa actcataccc acgtctgcgg tagttctgaa
    11881 tcagccattc ggcctgttct tcagtgcatg gggcgtgctg taacctgtca gatttgaatg
    11941 catgagaacg ccgcccgtgc ctgctggcaa agacggctga attatcagaa ttgtataatt
    12001 tggtatcgtg cgccatcggt tgtctctgct ggcgcagcag gtgccagttg ttcaggctgg
    12061 cgtgcgaatt gtaaaccaga atgccaggaa aaaacaaaac ccgccgaagc gggttaagtg
    12121 cgggtgcgtt gaggatgcct gactcatcag aggtggcgag ggatttctcc ctcgccaggt
    12181 ctcttactcc tcaggttcgt aagctgtgaa gacagtgacc tccgtctggc cggttcggat
    12241 tcgtacctcg cagaggtctt tcctcgttac cagtgccgtc acaatgacgg taatacagat
    12301 gacgatcagg gcgactaaca tcgccttttg ctgcttcata gcctgcttct ccttgccttt
    12361 cggcgtgtaa gaggctaacc tacatgtgac tagcatgaaa ttggcctcag attaatgtta
    12421 agcgtcctgc aagacgcata atgttaactg gggcttttat ctgtctgcct tacggtggca
    12481 tgcccgaggc agacagcctc aagcacccgc agcaattcta cttaactctc gctttaccgc
    12541 aaaccgtttt tacccgatat gggaattccc atatcgtaat gaattcagtt ccctagtcga
    12601 tccatcaaaa acacaaccag gcagtaaaca cccacaacag caataacagc cagcgcacct
    12661 tccattgcca gtgagatatc atccgacaca ttccctcctt tggtgttaat cccggcgaac
    12721 gcttttaccc acaccgacaa ataacatata ctaaaaaagc gatagccata gcaacgcctg
    12781 taattgcaaa tgcttcaggc cagatcattg gcgcacctcc tgcggcggtt ctggtaacgg
    12841 catccagtcg gttacattgc ggctctgtgt ttcgaaaaat tcatcaccat tacggactac
    12901 atcaaaaaac tcaccgtctc gatattgcgc ataaagaacg aatgcgccat cacataaaat
    12961 aattacgtgc tgaccatcat ccggcattcg ctcactacag cttatccaac catccggagt
    13021 taccggaagc gagaacggca gcacatctct gtgaacaagt ttttgctgtg acaggttatc
    13081 cagaactttc tgtactgctg catcaccgaa tacaccaagc gcatctgcca taactcctac
    13141 aacctgataa gcctcagcgc ataccgtgga taaaccatcc ggaattaccg gagagttgcc
    13201 gggttcttta atgtgcaagc gaggctcacc atcttttggc tcaggccact ggcgctccat
    13261 gttgatcttc aatttatctt ccatagcagc ggtaatttca gcatcgctga tgccagcacg
    13321 gcgctgtgca tcccacaaca gaaactgcat atcagcccac tcgctaagat cgtctggttc
    13381 ggctgcggct tccagtgcct cttttgagag gtgtttcagt ggaccaatgg ggccaacgca
    13441 gccaaatgtg gagtcagacc atttggcatg ctcgtggcga atctgttcgc gttccagtga
    13501 tgccagtgca attcgtgcca gttccatttg ttcgccacga gtaagcccgt tttcaagcgg
    13561 atttttaatg aacaattcaa tacgttcttt ggtaatagtg gtcatagcta tttcacctta
    13621 atctcaacat ttcgcagttt tagctccact ggcagttctg actttccggt taaagctaat
    13681 gcgagatttt ctggagtaat gagagctgtt attgttctcc ccatcgccag acgaataatc
    13741 attcgtatct cgcgatcgtc acatgctccc ggtcgaacaa ttgagatttg tccgttcata
    13801 tcactctcct ttgatgcgaa tgcccgttgc aatgctgttt atgatgctgt cagtgcatgg
    13861 ggtagaaagc tgggcatctc cagcaatttt catgacctca acatctgcat atcgaatacc
    13921 gaggtgtatc agaccggcta tgcctgactt aagccgagca ttttccataa atagaacttt
    13981 tgcccgctgt ttttctgctt caagctcaac gcgcagcttc cctaccgtta gcgcaatatc
    14041 ctcgttctcc tggtcgcggc gtttgatgta ttgctggttc ctttcccgtt catccagtag
    14101 tgccagcacg gtagccgggt tagcctctgc tatgaattca gcgtttgcat aagcctgagc
    14161 atctgtttca atcaggcagt taatatgaca ttccgcaatc acgccaccgg gttctccttt
    14221 ccatttttga caaacaaaaa ctcctgttaa attgccgtgt tggttaacag atgtatgccc
    14281 tacgatgtag cttcctttag ttgctttctc tgccttttca cgcagtacct gatagttaat
    14341 ctcgctcatt tttctcttca ttccgttata caagaattac aacgtcacct ctgctaatta
    14401 cgcgagctgg atcccctggt tccatgctgt caatcccgaa ggcttcgaaa aatgcatcca
    14461 ttgccttctg gcgttgctcc tgcttacggc gtttattcca tttttgaaca acagtgacag
    14521 ccatcgtccg ctgcagaaca tgatgtagag ataaccaaga agtgctaggc cggtgttcag
    14581 ggccatatca atcgttatcg ccgggtcaat attcactgcc cacctcctga aaaatcaccg
    14641 catggcccag tttctccgcc agtgccagct cagcccttgc acctgccgac tgctgccagc
    14701 ctttcagcat gtaaaccgca tccacgcaac ggagcattgc catgcaaata tccatgtagt
    14761 gtggttgagt cagcccgtcc ggaagtactg ccgggtttaa aacggtatgc ccttcccgtt
    14821 tcagcgcatc ttccgccctg tgaaacgcct cgcggttgaa attttcatat cccgtcatcg
    14881 gaccggcgat ataaatcctc accctcactc ctgaaccctc ctgtcgaaat aaacgtagtt
    14941 attcactgcg cccaacttca tcccaaactt ttcggcaatt tcccgtcggg gtacgccacg
    15001 ctgatgcagt tgccgcgcca gctcaatatc acgctgtgaa tattttgccg acgggtgaaa
    15061 atcaccccgt aaaatcatgc tgatacccag ttcccgcgct ttcgtcctga cggctgactc
    15121 actacgacca atcagataac cgatgctttc gactctcatc gttcccgcac actgccggag
    15181 tatcaggatt tcagcccagc gccacttctt ccagccactc accgctgctg ctctctggtg
    15241 gcggtaatat cccggagaat atccctgtgt ttgttcagtt cccgcagcgc agcacagact
    15301 cgctcccact tctggacatc acttttcgcc cggcgcagct cgcggttagc cacatgcagc
    15361 gatggtagaa tcaggtcatc tgctttcatt tcggtgatcg atggctgtaa cctcacaatg
    15421 tcttccacga tttctgtttt catttcttcc tgtgccatca tttcctgtac tggtaacgca
    15481 acacctgctg gctgaggaaa ggctttacca tcggtttccg ctacggatgc agcttccggc
    15541 tctgccggta aatcagcgcc cggtatgcag taacgaaatt taccaccctg attcacgcga
    15601 atcagacgcc ctttgctgat tgccatggcc agcgatgaat tcgcccggcg ggaggtaatc
    15661 ccgaacatca gtgccagctc atccgccgtt tgtgggccat gttgttcaat cgcctcagtc
    15721 agcatttgcg ctgtcacttt cggtaccggt gacaccggtt cactttcacc agcctgaatc
    15781 agccaccaca tcgaaccctt gttatccgct tcaccgcggc gcttcagttt ccacagttcg
    15841 ttgaccgcat cttcacggct gattccaagg cgggccgcca ctacctgtga agaggctctt
    15901 ttcagtgctt tcagtgcgtc aaatacggtt tccattaaaa tttcctccga caaaatcgtt
    15961 tctcagactc aaataaaacc agctgccttc cggcgttcgt attcctgttt cagccgttca
    16021 attggcgttg gcccttgcgg gtgtttcgcc ccttccagtt gtcgtcgcac tggcggaaca
    16081 ctcatcccgt taccaacatg ctttgcccat ttcgtcagtt gccgttccgc aagtcgtttt
    16141 aactcaccct gcgtcatctg gcgctcaatc cctctggtac gcatttcgag gcagatgtgg
    16201 tacagcacag gctgaggcca cgggtattta tcactcccgt cgtatcgcca ggattcattg
    16261 cgccagcgcc ggtactcttc catcacggca tccaccgtaa gaccaaatgg atttgcccca
    16321 ctctccgaaa tcagcgcaac aaactcagcc aggtccgggg gccacgtttc acccgcccgg
    16381 cagcggtcca tgcactgacg gcagaccagc cggatttgct gttcagtcat cgcgccaatc
    16441 tgggcaatcc agagcttcga aggtgcggcc ccgttcttct gggtccagcg gttcgaatac
    16501 acctccccca taagctccca cagcttccag gccatttccg ttgctgataa atccgttgtc
    16561 tcgttcccac tgttcgcgtg ctgcccggat ttcctgaact gcccgtgatg ccgtgccacc
    16621 tgatgctgca tggcttaccc ccttgctgac tggttttacc tgtgtcctga cgtgctgcac
    16681 gtggcgggca aatttctgct cccactgaac ctgtgtgaaa accttcccct ccgccatcca
    16741 gtaatcccgg aatgcggcaa gctctgcagg tgtaaattcc ggctcaggca gagccatacc
    16801 ccacactgct gcccgttgtc gaaaatccga cgacggctgc cagacagtag tcatcgaaaa
    16861 tttcccgatc ggttcgctca ggccgtccag gtattcaggt tcggctgtct gcaacggcgc
    16921 accatgcgac tcaccggttg gagcactctc gcgcatgcgc gcgttatgtg tggggtttaa
    16981 ttctgtatct gtatctttat ctgtcgtgac ttgtcgtgac agatgcgtga cacgtcgtga
    17041 ctcatcgtga caatcagcat catatttccg cagcttttcg cgctcccgct gcgctctctt
    17101 gcgctctgcc ggggattttg ccgtttgcga aacgttacca ttgtcctctt tcagcacctg
    17161 acgtttttcc catccggaaa taaggtcacc atccagaacc cgcccctgca ttgcatgcaa
    17221 aattgaatca attacgtctt ccgtcacatc aagcgcactt gctaaatctt ccgtcgtgac
    17281 atcaatgtga ccacgtagtg acacgccgtg acatgtcgtg acatttcgtg acgcgctcac
    17341 cagaaggtgg atatacactg ccatcactgt tgcgattggc tgtcctgata ccctggcaat
    17401 cgttcgccac ttggggtcat ttggcatgtc atgccacaat ctgagccagg cattagccat
    17461 actcacctct tctgataccg aactttaccc acgaacttcc ggaagaaatc cggtataaat
    17521 attgttggtc aatgcacaac aacagcatta ccaggctgac caccactgtt agtcagggtg
    17581 ccccaggcga tcgctacagc gacaaaatca tccacatctt tcaccagccg atcccgtcgt
    17641 tcgacgatct cccggtaata ttcagaactg tgactgcgca tacgggccac cagcaaaggc
    17701 ggcatcgcct tttcgatcgc cggtaacaga gcctgcattt tttcaacagc atcaggggtg
    17761 tctttatcca gccaacggaa aattttctgg gtattacggg ccagggcttc cggatggctg
    17821 tcgtcataca gttccgggaa cgtcattccc agctcgaaat acgctttggt aattttcgca
    17881 gccggtactt tttcgccgtc cggatgcgcc cagacattca tcgccatgcg gatgtgttca
    17941 tgcttgattt tcatgaatca actccggtgc atttgatgtg ttaaccttga atccaacagg
    18001 taaaccatcg gttgggttag gataaatatc agggcgaatt tcatgcgggg taacttccca
    18061 cttcattagc tgacataacg gaattacctg ctttggggga acgtcaaagc taaaccattg
    18121 ccaaacagtc tgttgagcga cccccatata acgacctatt tcagcttgag tgtatttctg
    18181 cctaattttt tcgcgagtgc tatctagcat tttgctctcc tctaaaaaac tataagcaaa
    18241 gcctacaata aaaaactgta cacgatcaac agttttttat tgtgatgctt ttaacagtat
    18301 ttacctgtaa aattgaataa tgatgaacgc cctagaagta tctatgtaca gaatcagcaa
    18361 gcttcttcag gaaactggat ggagccaggc tgagcttgcc cgtagaattg gtgtgacaca
    18421 acaaactgtt caacaatggg tcagcggtaa ggctacacct aaagcctcaa gtttggataa
    18481 actggttgag gttacagggc atccattgca ttggttttta ttgcctcctg aagaggggga
    18541 gcaaattttc acccctgaca cgatgaaaat tggtcctcgt caacgcgaac tgctccaggc
    18601 ttttagtgcg tttccagagg aagaccaaga aaaaatgctt caagaaatca aagacaagaa
    18661 aaaatcaatg gaagaaacca ttgcccggtg gttggcggca caaaaaagcc gccgggcgtg
    18721 accacagtac aagaagagga gttatgccat gagtacagcc ctttctccga tagtttcaga
    18781 attcgaaact accgaacaag aaaacagtta caacgaatgg ttacgcgcta aagtggcgtc
    18841 aagccttgca gaccctcgtc cctcaattcc acatgatgag gtaatggctg aaatggagaa
    18901 tcttattgct caaattgctg taactaacag gagcgagtaa tgttacccat tttgtggcta
    18961 ccatctgctc gcgatgattt ccgtcagatc gtagcctata ttgctaagga aaatattcct
    19021 gcagcacgca gactaaaaat acggattgaa acgtctgttt tagccctctc tgagcatcca
    19081 tatctatatc cgccaagtga tcgagtatcc ggtttgcggg aaattgtggt tcaccctaat
    19141 tatatcgttt tgtaccgagt agcagcttca agcattgaaa ttgcaaatat tgtgcatgcc
    19201 cgccgacaat ttcccttccc tatctgaact gaacaatttt cacactccct cattcgaggg
    19261 agtttttttt gcccaacaca acaattaaaa actgttgaca caaaacagtt tttaattgta
    19321 gattatttcc actaccccac cccgccccac agaacgccag gcaatacttc gagttacccg
    19381 gcagtggtca ggggttaagt agccagcccg aggcgtatga acatgacggc gggaacactt
    19441 tatataacag cgcagcaggt ttttagttcc gctaccccgg cgttaagggg aaatgaggtc
    19501 agcatggata ctatcga
//
LOCUS       DAZFKF010000095        19345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFKF010000095 DAZFKF010000000
VERSION     DAZFKF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.095"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(102..1580)
                     /gene="wzxC"
                     /locus_tag="ACHKA1_003961"
     CDS             complement(102..1580)
                     /gene="wzxC"
                     /locus_tag="ACHKA1_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO5681539.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVVVFFLSDAI
                     GDVLNNPDLAPLIKTLSLAFVMIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTVGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(1582..2976)
                     /gene="wcaJ"
                     /locus_tag="ACHKA1_003962"
     CDS             complement(1582..2976)
                     /gene="wcaJ"
                     /locus_tag="ACHKA1_003962"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO5681540.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(3031..4401)
                     /gene="cpsG"
                     /locus_tag="ACHKA1_003963"
                     /gene_synonym="manB"
     CDS             complement(3031..4401)
                     /gene="cpsG"
                     /locus_tag="ACHKA1_003963"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO5681541.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTSLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(4594..6030)
                     /gene="cpsB"
                     /locus_tag="ACHKA1_003964"
     CDS             complement(4594..6030)
                     /gene="cpsB"
                     /locus_tag="ACHKA1_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO5681542.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGELTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     TRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(6033..7256)
                     /gene="wcaI"
                     /locus_tag="ACHKA1_003965"
     CDS             complement(6033..7256)
                     /gene="wcaI"
                     /locus_tag="ACHKA1_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO5681543.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQC
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAAERLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLHNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(7253..7732)
                     /gene="gmm"
                     /locus_tag="ACHKA1_003966"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(7253..7732)
                     /gene="gmm"
                     /locus_tag="ACHKA1_003966"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO5681544.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(7735..8700)
                     /gene="fcl"
                     /locus_tag="ACHKA1_003967"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(7735..8700)
                     /gene="fcl"
                     /locus_tag="ACHKA1_003967"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO5681545.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(8703..9824)
                     /gene="gmd"
                     /locus_tag="ACHKA1_003968"
     CDS             complement(8703..9824)
                     /gene="gmd"
                     /locus_tag="ACHKA1_003968"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO5681546.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(9851..10399)
                     /gene="wcaF"
                     /locus_tag="ACHKA1_003969"
     CDS             complement(9851..10399)
                     /gene="wcaF"
                     /locus_tag="ACHKA1_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO5681547.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(10415..11161)
                     /gene="wcaE"
                     /locus_tag="ACHKA1_003970"
     CDS             complement(10415..11161)
                     /gene="wcaE"
                     /locus_tag="ACHKA1_003970"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO5681548.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSS
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRRLKVQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTGKTKALYNKS"
     gene            complement(11287..12507)
                     /gene="wcaD"
                     /locus_tag="ACHKA1_003971"
     CDS             complement(11287..12507)
                     /gene="wcaD"
                     /locus_tag="ACHKA1_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO5681549.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNIHPK"
     gene            complement(12482..13699)
                     /gene="wcaC"
                     /locus_tag="ACHKA1_003972"
     CDS             complement(12482..13699)
                     /gene="wcaC"
                     /locus_tag="ACHKA1_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO5681550.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(13696..14184)
                     /gene="wcaB"
                     /locus_tag="ACHKA1_003973"
     CDS             complement(13696..14184)
                     /gene="wcaB"
                     /locus_tag="ACHKA1_003973"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO5681551.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNSVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(14187..15026)
                     /gene="wcaA"
                     /locus_tag="ACHKA1_003974"
     CDS             complement(14187..15026)
                     /gene="wcaA"
                     /locus_tag="ACHKA1_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO5681552.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(15119..17281)
                     /gene="wzc"
                     /locus_tag="ACHKA1_003975"
     CDS             complement(15119..17281)
                     /gene="wzc"
                     /locus_tag="ACHKA1_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO5681553.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAYRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(17284..17727)
                     /gene="wzb"
                     /locus_tag="ACHKA1_003976"
     CDS             complement(17284..17727)
                     /gene="wzb"
                     /locus_tag="ACHKA1_003976"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO5681554.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(17733..18872)
                     /gene="wza"
                     /locus_tag="ACHKA1_003977"
     CDS             complement(17733..18872)
                     /gene="wza"
                     /locus_tag="ACHKA1_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO5681555.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            19181..19330
                     /locus_tag="ACHKA1_003978"
     CDS             19181..19330
                     /locus_tag="ACHKA1_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681556.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
BASE COUNT         4526 a         5342 c         4818 g         4659 t
ORIGIN      
        1 cgcgtcttat caggcctaca ggtcccgagc acagaaccgt aggatggata aggcgtttta
       61 cgctgcaccc ggcaaccgtt gtcggagcct aaaacaacca ttcaccccgc ccgtaaaagc
      121 attttcattt tttcgctgcg acaaaactga cgcttcactt ccaccaccag cggatggcgc
      181 gacagcacaa tcatcaccac aaacgccagc acgcccgcgg ctatttgcac cgccagcagc
      241 atccccagcg ccagttgccc tttcagcaca atgcccagcg cataactgac caccagcgtc
      301 ggcagagaga gataaaacgg cagccacaaa ctcaggatgt actggcgata actggaaccg
      361 agcaccggtt taatcatcac gaagtaactc agaatggtgt tgataatctg caccagcagg
      421 aagccaaccg tgacgccgat tgcgcccgcc atctgcccac caataacaat cgccggaata
      481 aacagaaatg ttttgaatac gttgaattta aagctgatat cgacccgcgc tttcgccatc
      541 agcagcgaac caatcgggtt acccaccgaa cgcagcagac ccaccacaca cagcaattgc
      601 agcaccggaa taatgctgtt ccacttctca ccaaagacca gcggtacaaa gttattcgac
      661 accaccatca gccccagcag agccggaaag ttgataatcc ccactaccga cagcagcttg
      721 tagaagttaa cgcgcagctt ttcggtgtcg tcctgaattt tggcgaatgc cggaaacaac
      781 acgcgggtga tgattgggtt cagcttcatc ggtggcacaa cggccacgtt gtacgccagg
      841 ttgtatcccc ctgccacgcc cgcgccgaga atacgcgcca gcaccagcgt tgaaaggttg
      901 gtattgagat agttgatgat gctgtccgcc gtcagccagg caccaaagcg taagttcggt
      961 gccaccgacg ccagcgagaa atgcagaccg gggcgataaa ttttgcggcc aaagtagcca
     1021 aacagcagcg ttcttaccgc actattgacc agataaccga ggatcgcggt catcgccagc
     1081 ggccagaaat gggcgctaac caccgtaaaa gtgaagcccg ccagtactgc gctggtttcg
     1141 atcatgccga ttttgttgaa ctccagctcc ttctgcatca gtgcgcggaa ctgctgcccg
     1201 tgggggatca tcacaaacgc cagcgacaat gttttaatca acggtgccag gtccgggtta
     1261 ttcaacacgt caccaatggc atcactcaaa aaaaacacca ccacgcacac cacgatcccc
     1321 agcccgacgt tcagccagta caacgtggtg agttccaggt gactgatttc ttttcgctga
     1381 ataatcgagt tagcgatacc aaagtcagaa agcgtatctg ccagcgcgat aatcaccagc
     1441 gacacggtca gcaaaccgaa ctggtggttg tcgataatcc gcgccagcac ggtcatctgc
     1501 accagcccga ggccgataat gatcaccgtg gcaatcgccg accacttcgc gccgctgatg
     1561 gttttttcac gtaagctcat atcaatatgc cgctttatta acgaagccct tgaataccgt
     1621 caggaaaacg attttgatat cgaaccagac gctccattcg cgaatgtact caaggtcgaa
     1681 ttcgacgcgt ttttccattt tctccagcgt gtcggtttcg ccgcgccagc cattaatctg
     1741 cgcccagcca gtaatgcccg gtttcacctt atggcgcagc atgtagcctt caatgagctg
     1801 gcgatattgt tcgttatgcg ccactgcgtg cggacgtgga cccacaatcg acatcccccc
     1861 ggtcagcaca ttgataaact gcggcagttc atccagcgaa gtgcggcgca gaaagttccc
     1921 cactttagta acgcgcggat cgttctgcgt cgcctgagtc accactttgt cgttctccat
     1981 cactttcatg gaacggaact tccacacttt gatcggcttg ccatccatgc cgtagcgagt
     2041 ctggcggaaa ataaccggcc ccggtgaact cagtttcacc gccagcgcaa tacagcacag
     2101 caccggggaa atcagcagta aaatcagcgt tgccagcaca atgtcttccg cacgttttag
     2161 caggcggtta accccggaaa gcggcgtgtc gtacagcggc accacaggta cgccgttcat
     2221 ctcttcgata cgggaatgaa gaatattgaa ggtaaaaaca tcggggatca gcagcaccga
     2281 acaggtggta tccgccagtt gatggaccag ttttttcact cgcgcgccgt cgcacatctg
     2341 catcgcgata tagacgttat gaatcttgcc cgcttttgca tcctcgacca gctgttgcag
     2401 gttacccgcc cagtcgttag aaacgccgcc cggttttggg tcgtggtata cacccaccac
     2461 ttcaaaccct aaccacggct ggttacggaa gctctccatc agcatttgcc cggcggctaa
     2521 atcccccgcc accgcgacca tgcgcttgtt atagccatga ttacgcagcc agcccgcccc
     2581 aatgcgaata cacgaacggc aaaccaccag tccgatgctg gtcagcccat accacgccag
     2641 ccagattttc agttgcgtgt cgaaatcatt gttgaacgcc accagtccgg cgctgaaaat
     2701 cacgcttaag gtccagtttt gtagcaacag ggcaaattct gtcgctgccc gaacaccgcg
     2761 ccatgagcga taaaaatcgg tgatgccacc cagcatctgg aacaccacca gcgtaatcag
     2821 cgccaccaac aggtgcatgt agaggaatga cagtccgctg acttcgcaaa ccagccatag
     2881 tccggcaaac atgatggtga tatctgaaaa gcgttgcacc atagagatta acgatgcatt
     2941 ggttttcgct cgctcgcgct tttttagatt tgtcatcgtt gttcctgtta ttagcccctt
     3001 acccgcagtg gggtaaggga agatccgaca ttactcgttc agcaacgtca gcagagttcg
     3061 cgttcgcgct tccatcagcg gcacatcacc gcgcgattcc acattcaacc gcaccaccgg
     3121 ttcggtgttg gaggagcgca ggttaaagcg ccagtcggca aaggtcatgc tgatgccatc
     3181 ggtgcgatcc accgccagcg cctcacggct aaaatgctgt tcgacgcggt taatcgcctc
     3241 aacggggtgc gccagtttgc tgttgatctc accgcttgcc ggaaacgccg ccatccggtc
     3301 gcgcaccagt tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca
     3361 cgggatcatg ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc
     3421 gccaccgtag atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt
     3481 cgacatcacc ggcgtgccgc cagcagcagt caccacatca acagtgttcc aggagagacg
     3541 tggatcgtgg atgatcttcg cgccgggatt tttttcgagg aacgcttctg ccagcaggcc
     3601 gacaatgtag tagccctcga taaactgccc tttttcgtca aacaggaaac agcggtcaaa
     3661 atcgccatca aaagcaatgc ccatatccgc gccgtgttta atgaccgcat tgcgggtgtc
     3721 gtcgcggcat tccggcagca gggggttagg aataccgttg gggaagttgc cgtccggtgt
     3781 gttgtgtact ttgattaatt ccacgggcgc gccgagggct ttaaagcggg cttcaatggc
     3841 gtccaccacc ggacccgccg cgccgttccc ggagttgatc accagcttga gcgacgtgag
     3901 gtttttgacg ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca ggttgatttg
     3961 ctgatagcga ccgcgtttgg tttcatcgac gggaggaaag tcgttggctt cagccaggcg
     4021 ctggacatca cgcagtccgg tatcaccgct gatcgggcga gccccttcgc gcaccagttt
     4081 catgccgtta taatccatcg ggttatggct ggcggtaact tcgatgccgc catccacgcc
     4141 gagatggaac gtggcgaaat aaatctcttc ggtgccggac atgccaatat ccagcacatc
     4201 gacgcccgca tcctgtaaac ctttcgccag cgccagtttt aaggtttcgc tggtgaggcg
     4261 gacatcgccg cctaaaacaa tggttttcgg tttaagaaat tcgccatagg cgcgaccaat
     4321 acgccaggca atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt
     4381 aaagcaggtt aattttttca tatcgttacc ctttttcagg caatagttgg ccctgaccga
     4441 aacggccaat tttgtttttg tcgttattta caccgcggtt tcgcattcat tgcctgatgc
     4501 gacgctgccg cgtcttatca ggcctacaaa accgaaccgt aggttggata aggcgttcac
     4561 gccgcatccg acaaacagcg cctgatgcga cgtttacacc cgtccgtagc gatccgcgaa
     4621 gcgcaccaca tcatcctctt cgagataaga gccggagcgc acttcaatta aatcgagcgg
     4681 aattttcccc gggttttcca ggcagtgcgt cgcccccagc ggaatataaa tcgactcgtt
     4741 ttcaccaagc agtttgatat caccgtcaat agtgactttt gccgttcccg cgacaaccac
     4801 ccagtgttcc gcgcggtgat ggtgcatctg taccgacaag ccttcgcccg gtttcacggt
     4861 gatgcgtttc acctggtagc ggtcgcccgc gtcgatagag tcatatttgc cccacgggcg
     4921 atagacttcg cgatgtaccc gatgctcatg gcgaccatcg gctttgatct gctcgaccac
     4981 ttttttcaca tcctgcaccg cgttacggtc ggcaatcagc accgcatctt tggtctgcac
     5041 cactaccaga tctttcaccc cgacggtggt tacgaggcca gattcggcat acacatagct
     5101 gttttcagtt ttgtgattaa tcacatcgcc gtggcaaacg ttgccctcgg cggtgtgggc
     5161 gctgatctcc cataatgaag accaggaacc gacatcgctc cagcccgcat ccatcggcac
     5221 cacaacggca tctgccgtac gttccatgac cgcgtaatcc accgactctt ccgggcaggc
     5281 cagaaacgct tcttcatcca cgcgaataaa atcgagatcc ggatcgacgg cactcattgc
     5341 tttttcacag gcatcgagaa tatccgggcg atattttttc agttcttcga gatagcgtcc
     5401 ggcgcggaac aggaacatgc cgctgttcca gtaatattcg ccacttgcca cataggcctg
     5461 ggcggtttcc agattcggtt tttcgacaaa ctgcgccact tcaaaggcca ccgtatcctg
     5521 ctcgcccgca gatacttcac cgcgacgaat atagccatat ccggtttcag gtagatccgg
     5581 cacaatgccg aaggtcacca gtttgcccgc ttcggcatat ggcatggcat tacgcacggc
     5641 ggcacggaac gcgtcttcat cggcaatcac atgatccgcc gccagcacca gcattaacgg
     5701 gtcgctctcc ggactatgac gtgttgccgc cagcgccgcc agcgcaatgg ctggtgcagt
     5761 gttacgccct gctggttcga gaataatgtt ctcggtgagt ttgttcagtt ggcgcagctg
     5821 ttccgcgaca ataaagcggt gctgctcatt gcaaatcacc accgggcttt cgcactccac
     5881 gccgttcagg cggcagatgg tggtttgcag catggtgagt tcgcctttca ggcataaaaa
     5941 ctgcttggga taaagtacgc gggaaagcgg ccataagcgg ctaccggagc cacctgccat
     6001 cacaactgga tagagtttcg actgcgccat aattatcccc gaatatcatt tataaattga
     6061 cgtaacacgt tctctttatc gagcgtgcgt tcggcatatt cacgtgccac cgtgttgtgt
     6121 ttgggcagca ggagcgcctg acgtatcccc gccaccagcg cctcgaccga ttccggttct
     6181 acgcaaaccg caatgcccgg aaaggtttcg caaagctgtc ccagttctgt gtgggcctct
     6241 gcggtaatca ccgcgttacc gcctaccgcc agaatattgg tcagtttcga cggcaatacg
     6301 gcatctgccg cgccgcgttt ttgcaccacc agatggcaat cgcccatctt cagcagtgcg
     6361 ggtaaagcgt catacgattg cagcgggaag aattgcatgt tgtgcagtcc acgctgctgc
     6421 gccatttttt ccagccgcgc tttgccgccg ccctgcccga caatggcaaa aatcagcggt
     6481 tcatcacgca ggcgttcggc agcttcaata acgttttcca gcccctgctt ttcaccaata
     6541 ttgccggagt aaagaatgat ttttttgtta tccggcaggc caagctggtt acgaagggca
     6601 tcaacatcgg cgtctgcaac atgctgaaaa cgggcgattt ccgaccagtt ggggaagaaa
     6661 atgacgttat ccgccgccac gcctttttcg atggctttat tcatcatcga acgcgaaatc
     6721 gtggagacgt tatcgacgtt atgcagtccg ctacgttcga acgccgttgc cagctgtgcc
     6781 actttgccgc ctttgccttt tccggcaagg cccagcccca gcatggcgtc cacttcgtaa
     6841 tcctgaatat gcagcacggt acgcgcacca gagagtttcg ccagcaggcg cattcccggc
     6901 gtgcaaaaca gcgttggcac tacgccgata atgcgatccg gcttccagcg acattgcgcc
     6961 atcaacggga aaaaactgct gacggcaaaa ctgccgagat gtaacaggcg tttcagggtg
     7021 ctcggctgtt ttggcacgta cagcgggcag cgccacaccg tagcggcccc ctcttctcgt
     7081 ttgtagcgcc aggcggaata gttctcgccc acctgccact gcgggtagta aggcggtgcg
     7141 gtaatgaccc gcacctcatg accttgtgcc gccagccatt ccaccatctc gccggtgtat
     7201 ttgccgatgc cggttaactc cggcgagtag ttaatgccgt agaccagtat tttcataatc
     7261 cgggtactcc ggcacgcttc tcagcgagaa aataggcgcg gctgttagcg tgaacattgt
     7321 cactggcgag cagcgcgtcc ggcgtcaacc agcggtaatc gtcatgctgc tcatccggca
     7381 gtaacagttc ttcttccgct actctgaagc gaaaaccgag caccacatag tgagtggtga
     7441 aatccgtgcc agagaagtta tcgtcataaa agtgctgcca gacaccgtaa aactggcctg
     7501 ctgttatcgg cagacgcagc cccagttccg ccatcgtcag ccgctcaaat gcggcttcca
     7561 gcgtttcgtc tttctgcacg cgccctcccg gcacaaacca gtaaccctgc gccgggcggt
     7621 tggttctttt gccaagcaga aactcgccgc gactgttctc gacaataaag tcgagagaga
     7681 caagcggagt ggagcgcact accgtggcaa agtcttcctg acgtaaaaac atcattaccc
     7741 ccgaaagcgg tcttgattct caaggaacca ctggtaagtg ctggcaagcc ccgcttccag
     7801 tgagatttcg tgataccagc caagctgatg caggcgcgtc acatccagca gtttgcgcgg
     7861 cgtaccatcc ggtttactgg catcaaaaac cacccgacct ttgtaaccca ccactttggc
     7921 gatggtttgc gccagctcgc ggatagtgca gtcaacgccc gtgccgacgt taatgtgcga
     7981 cagcatcggc tgggtgttct ccagccagac ttcgtgcgcc agctccatga catgaatgct
     8041 cgccgccgcc atatcatcga cgtgcaggaa ttcacgcatc ggtgtaccgc tgccccacac
     8101 caccacgtcc ggtgcattct gtgccgtcgc ctcgtggaag cgacgcagca atgcagggat
     8161 cacatgcgaa ttactcgggt ggaagttgtc atgcgggcca tacaggttgg tcggcatgac
     8221 cgagcggtaa tcgcgtccat actggcggtt gtaagattcg cacagtttga tcccggcgat
     8281 tttggcaatg gcgtaaggct cgttggtcgg ctccagcgtg ccctgcaata actcgctttc
     8341 tgccatcggc tgttttgcca gtttcgggta gatgcaggac gatccgagaa acagcagttt
     8401 gttcacgtcg ttctgatgcg cggcgtgaat gatgttgctc tcaatcatca tgttctgata
     8461 gatgaaatcc gccggatacg tgttgttggc aacaatgccg cccactttcg cggccgccag
     8521 atagacctgg tcaatacgtt cgctggcaaa gaaatcatgc acggcgcggc tgtccagcag
     8581 gttcagctcg tcgcgggtgc gtaataccag ttccacatca ccgcgctgtt cgagctgccg
     8641 cctgatggcg gaaccgacca tcccgcgatg gccagcaata aaaatgcgtt gtctactcat
     8701 acttatgact ccagcgcgat cgccacgtcg tagccgtgag atttcagcag agagtgtttt
     8761 ttcgccgctt cgaggtcatt agccaccatt tcagacacca tctctctgag ggtgatttcc
     8821 ggtttccagc ccagtttttc gtgcgctttg gtcgggtcgc cgagcagcgt ttcaacttca
     8881 gccggacgga agtaacgcgg atcaacggcg ataatcacat cacccggttt aacgcccggc
     8941 gcgtcatgtc cggtgacgga aaccacaatg cccttctctt caacgcccgt gccttcaaag
     9001 cgcagtttga tgcccagctg tgccgccgcc atttccacga actgacgtac ggagtactga
     9061 acgccggtag cgataacgaa atcttccggt tgttcctgtt gcagcatcat ccactgcatt
     9121 tttacgtagt ctttggcatg gccccagtca cgcagggaat ccatattgcc gaggtacagg
     9181 cacgattcca gcccctgggc gatgttggcg attgcgcggg tgattttgcg ggtaacgaag
     9241 gtttcgccgc ggcgcgggga ttcatggttg aagagaatac cgttgcaggc gtacatgccg
     9301 taggattcgc ggtagttaac ggtgatccag taggcgtaca gtttggcgac cgcatacgga
     9361 gatcgcgggt agaacggcgt ggtttctttc tgcggaattt cctgcaccag accatacagt
     9421 tcagaggtgg aagcctgata gaaacgggtt ttcttttcca gaccgaggaa gcggatcgcc
     9481 tcgagcaggc gcagcgtacc catcgcgtcg acgtcagcgg tatattccgg tgactcaaaa
     9541 gagaccgcaa cgtggctcat tgcccccagg ttgtacactt catccggctg tacttcacgc
     9601 aaaatgcgcg tcaggttgga ggtatcactc aggtcgccat aatgcagatg gaatttcggg
     9661 ttgcaggtgt gcggatcctg ataaatgtga tccacgcgct cggtgttgaa tgacgatgcg
     9721 cgacgcttaa taccatgcac ctcgtaacct ttttccagca gaaactctgc caggtaagaa
     9781 ccgtcttgtc cggttacacc ggtgatgaga gcgacttttg acatgtctta ttcctctgta
     9841 ttttttgaat ttattcagtt tcaacgcgtt cgcgtatcac cactgcggga ttcccccggc
     9901 aaaccacatt tgccggaagc gatttaaaaa cactgcttcg tgcacccacg acggtgccgt
     9961 cgccaatcgt gacgccaggg gcaacaaaaa catctgtcgc cagccagcat ttctcgccaa
    10021 tcacaatagg cgtggcgtta atggtgaaat gttgacttgc atggtcgtgg ctaccggtgc
    10081 ataaataact tttttgcgat atcaccgaat gtgcgccaat ggttatttca ccgagggtat
    10141 ataaattgac gtcatcgccg acccacgcgt aatcacctaa ggttaatttc cacggatagg
    10201 taatttttac tgacggacga ataactacgt tttttcctat ttttgcgccg aataaacgta
    10261 ataaaaaagc ccgccagcga tacaatactt gtggcgacca ggcaaatatt gttgcctgta
    10321 ctgcccacca taattgcact tttatagcgt tgccgccccg gaaccctttc ggcaccgaga
    10381 atccacttaa atcttgcatc gttttccctt atattcagct tttgttatat aaggctttag
    10441 tcttacctgt cgtacgctgg cgtaaatgcc aggataattc agcccagaag ccaggcacat
    10501 gtaatatttg acgttggact tttttggcgt cagcacacaa ttccatatta ttggtggtag
    10561 ataccccacc catagaaaat tcagacacca gtccattgag ttttttaaat gcataaccgg
    10621 ctttatacat tttggctgcc agcgcgtaat cagaagaaac tttatattcc aggtcataac
    10681 gccatttttt caagccggat accgggaaaa atatcgcctg atgactggcg ggcagactgt
    10741 gataaatata ccaacccggt ttggcgctac gtttaatttt atgcccgtcg ccaaaatcca
    10801 gtagcgcatc gccggtgatc atcacgttat ctttttgcac ttttaaccta cggacaaaat
    10861 ttgcggcatc ctgatgaaaa atatcacctg agttgagaaa caacgcgaac ttaccttgcg
    10921 ccatcgcaat gcctttgttc atggcgtcgt agataccgtt atctggctcg ctgacaaagc
    10981 gtaggttata gataccattg agattttcca ggtactcacg ggtgccgtca ctggaaccgc
    11041 catcgacaac aatccattcg aagctgatat cgtccgcctg cgccagatgc gccagcgagg
    11101 cgtgtgtttt gactatccct tcgaggttac gaaacgcgac agtgattatg ctaagcaaca
    11161 tgttttacct cgtaataccc gtcatacttc aagcggcatg tgcgttggtt ttcctcgctc
    11221 accccagtca cgtactcgtg tacgcttctg gggattcgct gcgtcaccaa cttcctgcaa
    11281 ctcggattat ttagggtgta tatttaacgc ttttcgcaaa ataaacggac agacgattaa
    11341 aaatgcatat tccgggctaa atatcgaacc ggtaaaaaac agcgataccg gagtaaaaag
    11401 atataattgc acgcgaaaat tgcggttatc accaaaagcg ttgatcatca tttttatcac
    11461 tttccccatg taccacagcg ataaaaacac cgcgaaccag gaaaaataaa taatcagcag
    11521 atacaaacca ttgtctatgg tttttccgac atccgcaccg ttaaatattc cgaatgatgc
    11581 gacatattca taaagtgagc caaatctgac tacaccgtca atatgggtca aagaataacc
    11641 gaccatcacc agcggaccga caatacgata atacgatgac gatccttccg tacctaaatc
    11701 gcccagacgg gtggaaatat aaggaaacgc gattaccaca ccaaccagga atacagccag
    11761 agaaatcaat gctaacggta actttttctt aatcgcctct ttattcagat attgaaatgc
    11821 ccactccagc aaataaaaca ggataaaggt cataacccct gaaaacgatc cggataatat
    11881 tatccctgcg agaatcatag catcggtttt aggcgttttg ataccaaact gtttgatgct
    11941 gagccaaatt gagattaatg ccagagcgaa aaatgccggt tcgaaataaa gtgctgtcgt
    12001 gcgcttgccg ccgaatttaa tgaaattcag cacatagctg ttgctgtaaa tcagatattt
    12061 cgaaatcgac tccataatac tgctgccacc ggtgaggata atttgcgcca tctccaccgc
    12121 cgccagcgcc accaccagcc cgaccaccag ataaaagaaa cgtaatatct tgcgatggtt
    12181 gtgtggcgaa atcgttttaa agcgaatact ccacaccatg ccaataatga tcacaatata
    12241 gacaaacagc atggttgaag tgacgtattt actggcatcc aacgactgac caaacaggta
    12301 gttaaacgcc gtgagtcccg cgccaatccc taaggcaatc atcaattttt taacgctgat
    12361 gcgttctaaa aacagcagta acaagacagg taaaaaagtg acgatggtga tggggaaact
    12421 ttcgccaagc tgagcgattt tgacgttaac cagcaggtag atcagcggca gcagcaggta
    12481 gctacagatt ctgatagaag ttgacatact cctccagcat ctgttgtcca ctgtaggcgg
    12541 cgcggctgcg ttggctgaac tcagccagcg tggtaccaaa tatcgcctgc gcgatttccg
    12601 gtttgcttaa ctggaccagt tgcagcacct cttcttcgct gacggtttta ccgccggatt
    12661 tttgcaacac ttcacgcgcc gcgtcgctat gggtggcaat caccggcacg ccaattgata
    12721 acgcctcaca caaaatcagc gggtagttat cgacgcgaga actgaatacc agcgcatcca
    12781 tctgattgag cgcgctcatc aacttgcgct tgtcagtttc aaagccgtga ttaaccacgt
    12841 tgccagcggt gaacggcgag aacttaccaa aggtatgcag ttcgattttg tcgcccagcg
    12901 ccatcatctc gcgtaccagt tgctggttag ttttgccgtc gtaacgcagg tcatgcgcta
    12961 ccaccgcgat tttcggctta ccctgagttt cgcgcaccgg aggcaaatcc gccagaatcg
    13021 cctcggttgc catatcaata ccgttattga taatccggca acgccctgga ccgtacaggc
    13081 tattgaaagc gtcagccaca tgctggctgg gggaaataaa ctgacagccc agcgccagca
    13141 tctcacggaa taactggcgt ttgcccgcca ccagctggtg cgcccgatca atcttcaccg
    13201 gcggatagtt atttaaggtc gggcatttct ggcagcccgt tttccagcct tcgcaaccgt
    13261 cggtaaaggc gcagcgtccg gttacactcc agtggtcgtg cagcgtccag accagagtga
    13321 cgtccggttt gtggtttttc accttttcgc aaaagcgcac cacgctctta agatttagcc
    13381 agtagctgtg cagcacatga aaatgaagga ccaccggacc cggtgtgcga gtaatggtgc
    13441 gatataactc attgaaattg ccaaacagat cgcgattaaa cagacgaaac agggcaatat
    13501 tcgccattgc ggtcatccgc ggcgtatgtt tgattacctg cggatagttc tgatggctga
    13561 cgctctcttt gccgcctttg ccgtagccgt agacaaaatg tgacgccagc ccctgttgca
    13621 gcgcgcgctg gtggagatct aacgccacac ctgctgcccc gccttccgcc agtcgcacat
    13681 taaattgcag aatattcatt taattacctt cactcgcgct ttttctccca ccaccagcgc
    13741 gttgtccggg acagagtcga gcaccacgct gcccgcgccc acggtgacgt tgttaccaag
    13801 cgtgatatca ccaagaataa tgacgttggc accgagttcg acgctgttgc caatgtgtgg
    13861 acatgccatg ttatcggcac cacgattgcc gatagtgacg ccgtggcgaa tggtgaaatc
    13921 atcccccgcc accacgtttt tattgatcac tacggcgtaa ccgtgatgga tagtaaagcg
    13981 gcggccaatg gtcgcggcgg cctggatttc ataaccgaaa aagcattcgg tgataatgcg
    14041 atacagcacc agcagcgggg ccgcccacag attgttgagg acgttctttt tgcgccacac
    14101 cgaacaaaaa tgagcgacgc gataggcaag aaccatgcag cacgggcgta aactccagct
    14161 gttggcgcgt agatcttcca gcatatttag cgcccccgaa taccatcagc cagacgtttg
    14221 ccgttacgca ccgacagcag cgtcagcaaa gtgcgccagg tcatacgttt attgcggatc
    14281 tggtagagag taaacagctg atatttttta ctggcacggt cgaatttgtc tttgtgcttg
    14341 cggtaaaagt ggaagtatcc ggagaatttt ttcggtgacg aggtgatctg catttcaccg
    14401 tgattgatat gcaggatctg cgtcgcttct tctactttcc acggttcgcc atactccacc
    14461 accatccgca ggaagatgtc gtaatcctgt gccgctttca gttcggtatc gaacaaacac
    14521 tctttgaaac gccacgccca ggtaaagacc tgattgccaa tgatattgcg tttatagaac
    14581 aggcggcgtg agtacggcga tttgggatac agtggtaagc ttgccggttg ggaataaact
    14641 tcgccctggc aaacgtaatc gttagcgtac aaaaaggcgt gagtgaccag ttgctgttta
    14701 tgggcgagga agacgctcag acggttgggt gtccattcgt catcgtcatc gatcccggta
    14761 atgtattccc cttgcgccag cataatagcc tggttacgta ccgcgcacgc cccgctgtta
    14821 atgtcgttgt gaatgtaggt aatacgcgga tcgttgaggg cggtgacgta ctgttgcagt
    14881 tgttcccaag aggtggagca atcatccacg atgatcatct cccagttgct gtagtcctgg
    14941 cgcaagaccg attttatcgc ccgaatcgcc agttgttggc ggttccaggt cggcatatag
    15001 attgagatca gcggattgtc tttcataggt tgttctcccg gcttatgagc tggtttgctt
    15061 gccggatgcg gcgtgagcgc cttatccggc ctacggggcg gtgcgaatgc aggcgtcgtt
    15121 atttcgcatc cgacttatat tcgtattcgt aatagccata atcctgatac gcgctggcgc
    15181 ggcggaagat ggagttcaga atcacccctt tcaccggaat accgttttgc tcaaagcggc
    15241 tcagactggt ttccacttct ttcaatgtgt tgaccgcata acgcgccacc attaacgtgg
    15301 ttccaacatg acgaccaaca attgccgcat cggtcactgc cagaatcggc ggcgtatcaa
    15361 tcaacaccag gtcatagttt ttactcgccc agttcaccag ttcggcaaag cgttcgctca
    15421 tcaacagttc agaaggattt ggcggcacct gaccgcgcgg gatcaggtca aatttggcaa
    15481 tagaggtcgg tttagctgcg gtagtaatat cgccctgacc aatcagaatt tccgacaggc
    15541 cattgacgtt attggtgccc aacagctcgt gggtgtagcc tttgcgcata tcgcagtcga
    15601 tcaacaacac gcgtttattg gtctggctga tcaccgccgc caggttggcg cagacaaagg
    15661 ttttaccgat tgacgggcta accccggtca tcatcaacac attgttctgc gcctgcatca
    15721 tcgcgaagtg cagactggta cgcagactgc ggatggcttc aatcgccaga tcggttggat
    15781 tccccaccgc cagtagctgg ctctgtttat agcgtttaac tcctttgatg gttttgacgc
    15841 tatcgcgcgc tttctgccac tccgacagcg ggatgctggc atagacgctg ataccgtgtt
    15901 cttccagcac ctgcgggctt tcgatgccgc gattaaacaa cgagcgcagc agcacaccca
    15961 cgatagagag catcaggccg aggataatcg ccccaaggat aatcagccct ttcttcggtt
    16021 ttagcacgcc aggctgggtg attgccgggt caacaatgcg cacatcaccg acggtgctgg
    16081 cctcggtgat tttcagctcc tgctctttat tcagcagttg catatagacc tgctgaccag
    16141 actcgacatc gcgggtcaga cggacaatct cctgctgggt tttcggcatc gccgttacgc
    16201 gaccgttaag tttggctttt tcgtcttcca gcgcctgacg tttctccagc agcgtgcggt
    16261 acgcggggtg aactttggtg tacagcttgg agatttccgc ctctttaaag gtcagttcgt
    16321 tcaactgcgc gtcgatgttc accatcgaat cgagcaccgc tttcgcttcc agcggcagat
    16381 caacagaatc tttatcctga cggtaggcat tcagtttgtt ttcggcaaca tcaaggcggc
    16441 tacgtacttc cggtaactgt tgcgcgagga aagcgaggct tttcgacgct tccgccgatt
    16501 tgcgctcaat attttgttcc tgatagttac gggcgatgct gttaagaatg tcgcggatct
    16561 gttcgcgatc ttcaccggta taagtcaggc tcagtacgcc tgcgtctttg ccgttctccg
    16621 ttaccgtcag gctgttttgc agttggttga tcatccccag cgtggagtat ttggtgacgg
    16681 taaactcact gcccgggctg gcgtgaatag cttcaaccat cagcgtgacg ccttcttttt
    16741 tcagcatctg gcccgcttgt ccacgggcgc taaagccgcc atcactgctc agggtgtagt
    16801 ttttgtcatc cagcacatta agcgtaaaca cctgatccgc catctctttc ggacggttaa
    16861 aggtagtcac tttcaccgtc tcgttctgac gtcccatcag gcgatcccag cccgcaccga
    16921 aaatcgggaa cgtgtttttg ctcaccgcaa tatcgaggtc gagatcgtcc accgttttac
    16981 caagcaccag gcgcgaacga atcaactgga tctcggcgtc cgatgcaggc ggtttgttgg
    17041 ctaacgccga tccgatatcc tgcactaacg aattgccgct gctttgctcg atttgtacca
    17101 gtgcgtcggc actataaatc ggcgtggcga agaaggtgta aaccacggca caaagggcga
    17161 atacagcggt gatgccaatc acccaccagc gcgcttcaat gacggtgccg accaggcgac
    17221 caatatcgat ttcatcactg cccgttaccg gagcggcatg ttgttttact ttttctgtca
    17281 ttcttatacc tgctctgcgt tcaatgcctg cgcccactgg cgggcagacc gttcaagtaa
    17341 tgtgtacacc gctgcaaacg tttcccggct tttgcgatac ggatcgggga tttcacattc
    17401 gttatcccag tgaccaaaca gcatcacttt gccgcgcatc tccggtgcca tctcgcataa
    17461 gcgttcgata tggcgctttt ccatggtcaa aatcaggtca tagttgcgac acaggcggcg
    17521 gctgatttgt cgtgcacagt ggccttccag agacagttga tgttctgcgg cgacgctgat
    17581 agcggtagga tcagcgccct taccgaccag cgcgccgagt ccagcggact ccacttttag
    17641 ctccgggtga taacgttgca gtaagcgttc cgccgtcggg gaacggcaaa tattgccgac
    17701 acagacaact aagatgttgt taaacatgac gattaccagt tatgaatgtc gctggctgta
    17761 tccgtcatgt aacggacacc gctaatagtt ggcagcaact gattgatcag acggttccag
    17821 cgggaaaccg gggcggtggt gacatacacc acgtcataag gttgcaggcg gaattctgtc
    17881 gccatcacca gcgacgtggc atcggacata tccagctggt agatattggc aatcttgccg
    17941 ttacgcccgc cctcgccttt cagcggacga atgacaaaga tgccgctggc gttggaggtg
    18001 gtcatgtcaa tgccttcagc attgcccaga gcttcagtca gggtcatgcc gctaaagtcc
    18061 attttcaggg tgctctgttt cttcacttca cccatcacaa atactttcag atcatcatta
    18121 cgcggcacgt agagaatatc gccggggtaa agcaggcgat tctggttgag gtcgccgttt
    18181 tgcatcagcg cctgcaaaga aatgcgctct tcacgaccat tgtgtgttag caccacgttg
    18241 cgccagtcag cggtgtcggt caggccacct gcggcgttga tggcgtcgag aatggtcagt
    18301 ggtacgttgg tgatcgcctg ttgaccagat ttattcacct gacctgatat ataggccttt
    18361 tgtgagcgga aggcggcgat attaacgtcc acctgcgggt cagcgatgta cgtcgctaag
    18421 cgcccggtaa tatcactgcg gatttcagcg agcgttttcc cgactacgtg aaccttgccg
    18481 atatacgggt aaaacatagt gccgtcaggc tgtacccagt tgccggtgtc gctggagctg
    18541 cggtactgac cggctggcgt ggtgagttcc gggtgatccc agacggtgac attcagaacg
    18601 tcgcccggcc cgacgcgata ctggtaattc gcgatctcac tttccagcgt catattgggg
    18661 cgcgctacgt tgggtcgtgg acgtaattga tcaatcaggc gcggggtcaa cggataaaca
    18721 ttcaccattt tgtcgagatc gaaatcagcg tcctgctgtt tgatgacgtc tttgcccatc
    18781 gtcgacatat tgctgcccgg aagtactgtg caaccgctta tcaaggttac tgacaccaat
    18841 aatggcatca atttcatttt ggatttcatc attgtttatt tatcactttg gcagagtaat
    18901 tatcctgtgc actattaata gcaatgtcgc catgcacatt taccttgcag ttaattgaat
    18961 aaaaatttaa ctggcatcag tcctaaaaaa attgatttca tccgcaggct attgacagaa
    19021 taattcagac tggtctttca ggcatccaga cacgctaccg cccctggctt tttagctacc
    19081 aatacactga tttagcttaa tttttaaaac cctctcaaca tacagttgtt gatgagaaag
    19141 ggttattgcg gaaattaact tccgaatata aggtgacatt atggtaattg aatattggct
    19201 ctccaataat gcaggaggaa gtgttacagc taacggaata gcaggcaagg taacaattcg
    19261 gcaattggct atttttaaga attatattaa gtgtcattca atatggtttt taggagtttc
    19321 tttaggttga caatatttaa tattg
//
LOCUS       DAZFKF010000096        19192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFKF010000096 DAZFKF010000000
VERSION     DAZFKF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.096"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACHKA1_003979"
     CDS             complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACHKA1_003979"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO5681557.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVIAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="ACHKA1_003980"
     CDS             complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="ACHKA1_003980"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO5681558.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3243..4235
                     /gene="xylF"
                     /locus_tag="ACHKA1_003981"
     CDS             3243..4235
                     /gene="xylF"
                     /locus_tag="ACHKA1_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5681559.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            4313..5854
                     /gene="xylG"
                     /locus_tag="ACHKA1_003982"
     CDS             4313..5854
                     /gene="xylG"
                     /locus_tag="ACHKA1_003982"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO5681560.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5832..7013
                     /gene="xylH"
                     /locus_tag="ACHKA1_003983"
     CDS             5832..7013
                     /gene="xylH"
                     /locus_tag="ACHKA1_003983"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO5681561.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSTAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7091..8269
                     /gene="xylR"
                     /locus_tag="ACHKA1_003984"
     CDS             7091..8269
                     /gene="xylR"
                     /locus_tag="ACHKA1_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO5681562.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8377..9201)
                     /gene="bax"
                     /locus_tag="ACHKA1_003985"
     CDS             complement(8377..9201)
                     /gene="bax"
                     /locus_tag="ACHKA1_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO5681563.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9521..11551
                     /gene="malS"
                     /locus_tag="ACHKA1_003986"
     CDS             9521..11551
                     /gene="malS"
                     /locus_tag="ACHKA1_003986"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO5681564.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11729..12982
                     /gene="avtA"
                     /locus_tag="ACHKA1_003987"
     CDS             11729..12982
                     /gene="avtA"
                     /locus_tag="ACHKA1_003987"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO5681565.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLHEKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13133..13606)
                     /gene="ysaA"
                     /locus_tag="ACHKA1_003988"
     CDS             complement(13133..13606)
                     /gene="ysaA"
                     /locus_tag="ACHKA1_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO5681566.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13708..13848)
                     /locus_tag="ACHKA1_003989"
                     /pseudo
     CDS             complement(13708..13848)
                     /locus_tag="ACHKA1_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
     gene            complement(13850..14665)
                     /locus_tag="ACHKA1_003990"
     CDS             complement(13850..14665)
                     /locus_tag="ACHKA1_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5681567.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            14891..16291
                     /locus_tag="ACHKA1_003991"
     CDS             14891..16291
                     /locus_tag="ACHKA1_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5681568.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNQLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            16302..18272
                     /locus_tag="ACHKA1_003992"
     CDS             16302..18272
                     /locus_tag="ACHKA1_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFO5681569.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNISRVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIVVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(18402..19142)
                     /gene="yiaT"
                     /locus_tag="ACHKA1_003993"
     CDS             complement(18402..19142)
                     /gene="yiaT"
                     /locus_tag="ACHKA1_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO5681570.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
BASE COUNT         4782 a         4959 c         4757 g         4694 t
ORIGIN      
        1 ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt tgctgataga
       61 ggcgacggaa tgtttcacgt cgtggctgat aagcggcata acgctgcgca tctggtagat
      121 gcgactgttc taacggtagt tgcggcaaca actcaatgag cgatttctct ggattcgccg
      181 caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc gtacggtaat
      241 cgagctgctg accgctgata tccgccagca tctgacgcca gtactcacta cgcgccccgc
      301 caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc atgcacgaca tccatgccat
      361 ctgccagcgc ataacccacg ccttccagca ctgctcgcgc cagttcattg gggccatgtt
      421 gatgagtcaa accaaagaaa acccctttcg cctggggatt attgtgtggt gtacgctcgc
      481 cggaaagata aggcagaaac caaactggct cggcactttc atcagcctgt tgagctgcag
      541 cgattaaagc tgggacattg ctcaggccgg ttaattttgc ggcccaatcc agacacgacg
      601 ctgcactcag cattacagac attaaatgcc aacgctgcgg tagcgcatgg caaaagctgt
      661 gtacggcgct ttctggcttg cttaagaacc cttcgctgac agcaaaatag acccccgacg
      721 tccccagcga taacattgcc tgattagcat caaccattcc cacaccaacc gcaccagctg
      781 cattgtcgcc accgcctgcg ataactggca ccgccgccat accccacgct ttcgcaactt
      841 caggtaacaa agcaccagta atttcgctgc cttcgtataa tgcgggcatc tggtcacgag
      901 ataagtcgca agcctgcagc atgacgtcac tccagtcacg ctttgcgaca tccagccaca
      961 tggtgccagc tgcgtcagac atatcgctgg caaactctcc cgtcatacgc agacgcaagt
     1021 aatctttcgg taataatact ttgtcgattt gacggaatat ctccggctca tgccgctgaa
     1081 cccatagcaa tttaggcgca gtaaatccgg gcatcatcag gttgccggta atcactcgtg
     1141 attgcggaac tctcgcttcc agcaaagtgc actcttgcgc acagcgcccg tcgttccaca
     1201 aaatggcagg gcgcaatacc cgttgttgag catccagtaa ggttgctcca tgcatctggc
     1261 cggcaatacc caatgcttta acgtcctgca gagaatgctg atcgcccaga gctttcattg
     1321 cgcgatcagt tgcctgccac cactgttccg ggtcttgttc cgaccagagt ggatgcgggc
     1381 gcgaaacggt cagcttttcc gtttgcgaag caaccacctc accctgctcg ttaagcaaaa
     1441 taacttttac gcccgaggtg ccaagatcta tcccgatata catatcgatc gttccttaaa
     1501 aagatgcccg gtatcgctac cgataaccgg gccaacggac tgcacagtta gccgttattt
     1561 gtcgaacaga taatgattta ccagattttc cagttgctcc tggcgaccac tctgatgcac
     1621 cggagacaaa ttatgttcct gagcatattt ggctaaatct gccagtgaca tttggccttt
     1681 caggatttgc tggcccaatt cgctattcca gccggaataa cgctgcgcga tgcgtttatc
     1741 cagctcgcca tcttcaatca tgcgcgctgc aattttcagc gccagtgcca tcgtatccat
     1801 cgcgccgata tgaccgtaaa acagatcata tttatcagta ctttgacgac gtactttggc
     1861 atcgaagttc agaccgccgg tggtgaaacc gcctgctttg agaatttcat acattaccag
     1921 cgcattctct tccacactgt tcgggaactg atcggtgtcc cagcccagtt gagcatcgcc
     1981 acggttcgcg tcgacagaac caaacaggcc aagcgcaatg gcggtggcaa tttcatgatg
     2041 gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg ttcagtttaa tctctttttc
     2101 cagaccaaac tgtttcagga agccatagac tgtcgcagca tcgtaatcat attgatgttt
     2161 agtcggttct tgcggtttcg gttcgataag caacgtgccc tggaagccga ttttatgttt
     2221 atgctcaacc accatttgca taaagcggcc cagttgttca cgctcctgac gcaaatcggt
     2281 atttaacagc gtttcgtaac cttcacgacc gccccacagg acatagtttt caccgcccaa
     2341 tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc gcccagctga agacttctgg
     2401 atctgggttc gtcgccgcac ccgcgccgta gcgagggttt gtaaagcagt tggcggttcc
     2461 ccacagcagc ttcacgccgc tctcttcttg cttgcctgcc aggacatcaa ccatttgcgc
     2521 aaaattattg atgtactctt ttaacgacgc gccctcaggg gaaacatcca catcgtggaa
     2581 gcaataaaat ggcacatgta acttgtggaa aaactcaaat gcgacatctg ctttacgctt
     2641 cgccaacgcc agtgcctcac caggctgctg ccacggacga ttaaacgccc ccacaccaaa
     2701 catatccgcc ccgttccagc agaaggtgtg ccagtagcag gcggcaaatc gcaagtgctc
     2761 ttccatacgc ttgcccaaca ccagttcgtc gggattgtag tgacggaatg ctaacgggtt
     2821 tgaggatttt gagccttcat aacgaacgcg atcgagctgg tcaaaatagg cttgcatatt
     2881 gaactccata atcaggtaat gccgcgggtg atggatgatg tcgtaatatt gggcactccc
     2941 tttcagttgc tcaattatgt tatttcacac tgctattgag ataattcaca agtgtgcgct
     3001 cgctcgcaaa ataaaatgga atgatgaaac tgggtaattc ctcgaagaga aaaatgcaat
     3061 aagtacaatt gcgcaacaaa agtaagatct cggtcataaa tcaagaaata aaccaaaaat
     3121 cgtaatcgaa agataaaaat ctgtaattgt tttcccctgt ttagttgcta aaaattggtt
     3181 acgtttatcg cggtgattgt tacttattaa agctgtcctc taactacaga aggccctaca
     3241 ccatgaaaat aaagaacatt ctactcaccc tttgcacctc actcctgctt accaacgttg
     3301 ctgcacacgc caaagaagtc aaaataggta tggcgattga tgatctccgt cttgaacgct
     3361 ggcaaaaaga tcgagatatc tttgtgaaaa aggcagaatc tctcggcgcg aaagtatttg
     3421 tacagtctgc aaatggcaat gaagaaacac aaatgtcgca gattgaaaac atgataaacc
     3481 ggggtgtcga tgttcttgtc attattccgt ataacggtca ggtattaagt aacgttgtaa
     3541 aagaagccaa acaagaaggc attaaagtat tagcttacga ccgtatgatt aacgatgcgg
     3601 atatcgattt ttatatttct ttcgataacg aaaaagtcgg tgaactgcag gcaaaagccc
     3661 tggtcgatat tgttccgcaa ggtaattact tcctgatggg cggctcgccg gtagataaca
     3721 acgctaagct gttccgcgcc ggacaaatga aagtgttaaa accttacgtt gattccggaa
     3781 aaattaaagt cgttggtgac caatgggttg atggctggtt accggaaaac gcattgaaaa
     3841 ttatggaaaa cgcgctaacc gccaataata acaaaattga tgctgtagtt gcctcaaacg
     3901 atgccaccgc aggtggggca attcaggcat taagcgcgca aggtttatca gggaaagtag
     3961 caatttccgg ccaggatgcg gatctcgcag gtattaaacg tattgctgcc ggtacgcaaa
     4021 ctatgacggt atataaacct attacattgc tggcaaatac tgccgcagaa attgccgttg
     4081 aattgggcaa tggtcaggag ccaaaagcag atacctcact gaataatggc ctgaaagatg
     4141 tcccctcccg cctcctgaca ccgatcgatg tgaataaaaa caacatcaaa gatacggtaa
     4201 ttaaagacgg attccacaaa gagagcgagc tgtaagcgtt acgccccagc gcggagcggg
     4261 ggcgtgattt ctctccatgc cgcgtgaatg aattggctta ggtggagtcg ttatgcctta
     4321 tctacttgaa atgaagaaca ttaccaaaac cttcggcagt gtgaaggcga ttgataacgt
     4381 cagcttgcgg ttaaatgctg gcgaaatcgt ctcactctgt ggggaaaatg ggtctggtaa
     4441 atcaacgctg atgaaagtgc tgtgtggtat ttatccccat ggctcctacg aaggcgaaat
     4501 tatttttgcg ggagaagaga ttcaggcgag tcacatccgc gataccgaac gcaaaggtat
     4561 cgccatcatt caccaggaat tggccctggt gaaagaattg accgtgctgg aaaatatctt
     4621 cctgggtaac gaaataaccc acaatggcat tatggattat gacctgatga cgctacgctg
     4681 tcagaagctg ctcgcacagg tcagtttatc catttcacct gatacccgcg ttggcgattt
     4741 agggcttggg caacaacaac tggttgaaat tgccaaggca cttaataaac aggtgcgctt
     4801 gttaattctc gatgaaccga cagcctcatt aactgagcag gaaacgtcgg ttttactgga
     4861 tattattcgc gatctacaac agcacggtat cgcctgtatt tatatttcgc acaaactcaa
     4921 cgaagtcaaa gcgatttccg atacgatttg cgttattcgc gacggacagc acattggtac
     4981 gcgtgatgct gccggaatga gtgaagacga tattatcacc atgatggtcg ggcgagagtt
     5041 aaccgcgctt taccctaatg aaccacatac caccggagat gaaatattac gtattgaaca
     5101 tctgacggca tggcatccgg ttaatcgtca tattaaacga gttaatgatg tctcgttttc
     5161 cctgaaacgt ggcgaaatac tgggtattgc cggactcgtt ggtgccggac gtaccgagac
     5221 cattcagtgc ctgtttggtg tgtggcccgg acaatgggaa ggaaaaattt atattgatgg
     5281 caaacaggta gatattcgta actgtcagca agccatcgcc caggggattg cgatggtccc
     5341 cgaagacaga aagcgcgacg gcatcgttcc ggtaatggcg gttggtaaaa atattaccct
     5401 cgccgcactc aataaattta ccggtggcat tagccagctt gatgacgcgg cagagcaaaa
     5461 atgtattctg gaatcaatcc agcaactcaa agttaaaacg tcgtcccccg accttgctat
     5521 tggacgtttg agcggcggca atcagcaaaa agcgatcctc gctcgctgtc tgttacttaa
     5581 cccgcgcatt ctcattcttg atgaacccac caggggtatc gatattggcg cgaaatacga
     5641 gatctacaaa ttaattaacc aactcgtcca gcagggtatt gccgttattg tcatctcttc
     5701 tgaattacct gaagtgctcg gccttagcga tcgtgtactg gtgatgcatg aagggaaact
     5761 aaaagctaac ctgataaatc ataacctgac tcaggagcag gtgatggaag ccgcattgag
     5821 gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa attggccgta ccgacatccg
     5881 gtggcttctc cgggctgaaa tcactgaatt tgcaggtctt cgtgatgatt gcagctatca
     5941 tcgcaatcat gctgttcttt acctggacca ccgatggtgc ctacttaagc gcccgtaacg
     6001 tctccaacct gttacgccag accgcgatta ccggcatcct cgcggtagga atggtgttcg
     6061 tcataatttc tgctgaaatc gacctttccg tcggctcaat gatggggcta ttaggtggcg
     6121 tcgcggcgat ttgtgacgtc tggctaggct ggcctttgcc acttaccatc attgtgacgt
     6181 tggttctggg actgcttctc ggtgcctgga acggatggtg ggtcgcgtac cgcaaagtcc
     6241 cttcatttat tgtcactctc gcgggcatgt tggcatttcg cggcatactc attggcatca
     6301 ccaacggcac gactgtatcc cccaccagca ccgcgatgtc acaaattggg caaagctatc
     6361 tccccgccag caccggcttc atcattggcg cgcttggctt aatggctttt gttggttggc
     6421 aatggcgcgg aagaatgcgc cgtcaggctt tgggtttgca gtctccggcc tctaccgcag
     6481 tagtcggtcg ccaggcttta accgctatca tcgtattagg cgcaatctgg ctgttgaatg
     6541 attatcgtgg cgttcccact cctgttctgc tgctgacgtt gctgttactc ggcggaatgt
     6601 ttatggcaac gcggacagca tttggacgac gcatttatgc catcggcggc aatctggaag
     6661 cagcacgtct ctccgggatt aacgttgaac gcaccaaact tgccgtgttc gctattaacg
     6721 gattaatggt agccatcgcc ggattaatcc tcagttctcg acttggcgct ggttcacctt
     6781 ctgcggggaa tatcgccgag ctggacgcaa ttgcagcatg cgtgattggc ggcaccagtc
     6841 tggcaggcgg cgtcgggagc gtggctggag cggtaatggg ggcattcatc atggcttcac
     6901 tggataacgg tatgagtatg atggatgtac cgaccttctg gcagtatatc gttaaaggtg
     6961 cgattctgtt gctggcagta tggatggact ccgcaaccaa acgccgttct tgattttgat
     7021 aaaaattttc tcaaagccgg taacgtatta ccggttttga gtttttgcat gattcagcag
     7081 gaaaagaacc atgtttacta aacgtcaccg catcacatta ctgttcaatg ccaataaagc
     7141 ctatgaccgg caggtagtag aaggcgtagg ggaatattta caggcgtcac aatcggaatg
     7201 ggatattttc attgaagaag atttccgcgc ccgcattgat aaaatcaagg actggttagg
     7261 agatggcgtc attgccgact tcgacgacaa acagatcgag caagcgctgg ctgatgtcga
     7321 cgtccccatt gttggggttg gcggctcgta tcaccttgca gaaagttacc cacccgttca
     7381 ttacattgcc accgataact atgcgctggt tgaaagcgca tttttgcatt taaaagagaa
     7441 aggcgttaac cgctttgctt tttatggtct tccggaatca agcggcaaac gttgggccac
     7501 tgaacgcgaa tatgcatttc gtcagcttgt cgccgaagaa aagtatcgcg gagtggttta
     7561 tcaggggtta gaaaccgcac cagagaactg gcaacacgcg caaaatcggc tggcagactg
     7621 gctacaaacg ctgccaccgc aaacagggat tattgccgtt actgacgccc gagcacggca
     7681 tattctgcaa gtatgtgaac atctacacat tcccgtaccg gaaaaattat gcgtgattgg
     7741 catcgacaac gaagaactga cccgctatct gtcgcgtgtc gccctttctt cggtcgctca
     7801 gggcgcgcgg caaatgggct atcaggcggc aaaactgttg catcgattat tagataaaga
     7861 agaaatgccg ctacagcgga ttttggtccc tccagttcgc gtcattgaac ggcgctcaac
     7921 agattaccgt tcgctgaccg atcccgctgt tattcaggcc atgcattaca ttcgtaatca
     7981 cgcctgtaaa gggattaaag tggatcaggt actcgatgcg gtcgggatct cgcgctccaa
     8041 tcttgagaag cgttttaaag aagaggtggg tgaaaccatc catgccatga ttcatgccga
     8101 gaagctggag aaagcgcgca gtctgctgat ttcaaccacc ttgtcgatca atgagatatc
     8161 gcaaatgtgc ggttatccat cgctgcaata tttctactct gtttttaaaa aagcatatga
     8221 cacgacgcca aaagagtatc gcgatgtaaa tagcgaggtc atgttgtagc gatgatgaga
     8281 attgtcggcg tcgcatcagg taactcagca caaccacctg atgcgattaa aagccttatc
     8341 cgaccgacaa gtcggataag gctcgaggaa atacaattac atatgcgccg cgattaaccg
     8401 ttggttatcc tggtacattg cgaacaggta gttgttataa ctcttccctt tggtcgagta
     8461 gcccttcagc ttgtgaatca tcgctgtggc agtcacttcc tgatcagctt tacgcagctg
     8521 cgcacgcgat ttacggaacg aagagtaagc cggatgcgta ttgaggttag tgacataggc
     8581 gctcaccgat tctttgacag aactaaactg tgagtaccct ttcactttac ctggcgcatt
     8641 ggtacaacgt cctttcatgc atttcatgcc gaacaggttg ttgttgttgc gcgccagctt
     8701 cgacgttccc caaccgcttt ctgctgcagc catcgtcgcc accatactgg tagggataat
     8761 gtctacgcgt tcaagcaagg tattccacgg gatttttcgc gtattaccag accacttcac
     8821 cttgtagcgt ttggcgatgt ctttcagacg cgcacgctca gcaggtgacc attggccctg
     8881 atactgtttt gaaatcagcc agttacgttc tgcagtaatg gctgcatttt ggctggtaat
     8941 gtaaggcatt acggtccgaa gaaacgcctt tttccttggt gttccggaag ggtattttcg
     9001 caaatcagga agtgaactac tctttgcact attgcgagaa tactcttgtt tactgcttac
     9061 ctgtttatta ctagctttag ttaagtggga cttttgactc gctgttgttg tgtgcgtctt
     9121 cgctaacacc tcactcgaaa acaccagagt gagtaacata agaatcatcg ccccatatcg
     9181 tcgtatggga gtcaaaatca tcaggtctcc tggtcggatt taatcattcc aacaccttat
     9241 atttttcaca aatttgagag ttgaatctca aatcatatca aaaatagctg tcaagagcac
     9301 cccaaggaat agtccaaatc tgaaactatg tcacgtgtta acgattcaga ttggcgctaa
     9361 atcgcagaaa atgtggggtt tatcgcaaaa ttcagccgtt ttttgcgcga gatcgctcac
     9421 ccttgcttct catcctgtgg acttaccgct cagggatgag ttttgtttgg cttatcgctg
     9481 gcaaactgtc tgaaatcgca gcaataagga ctcatccgcc atgaaactcg ccgcctgttt
     9541 tctgacactc cttcctggct tcgccgttgc cgccagctgg acttctccgg ggttccctgc
     9601 ctttagtgaa cagggaacgg gaacatttgt cagccacgcg cagttgccca aaggtacgcg
     9661 tccactaacg ctaaattttg accaacagtg ctggcagcct gcggatgcga taaaactcaa
     9721 tcagatgctt tccctgcaac cttgtagcaa cacgccgcct caatggcgat tgttcaggga
     9781 cggcgaatat acgctgcaaa tagacacccg ctccggcacg ccaacattga tgatttccat
     9841 ccagaacgcc gccgaaccgg tagcaagcct ggtccgtgaa tgcccgaaat gggatggatt
     9901 accgctcacg ctggatgtca gcgccacttt tccggaagga gccgccgtac gggattatta
     9961 cagccagcaa attgcgatag tgaagaacgg tcaaataacg ttacaacccg ctgccaccag
    10021 caacggttta ctcctgctgg aacgggcaga aactgacaca tccgcccctt tcgactggca
    10081 taacgccacg gtttactttg tgctgacaga tcgtttcgaa aacggcgatc ccagtaatga
    10141 ccagagttac ggacgtcata aagacggtat ggcggaaata ggtacttttc acggcggcga
    10201 tttacgcggc ctgaccaaca aactggatta tctccagcag ctgggcgtca atgctttgtg
    10261 gataagcgcc ccatttgagc aaattcacgg ctgggtcggc ggcggtacaa aaggtgattt
    10321 cccgcattat gcctaccacg gttattacac acaggactgg acgaatcttg atgccaatat
    10381 gggcaacgaa gccgatctac gaacgctggt tgatagcgcg catcagcgcg gtattcgtat
    10441 tctctttgat gtcgtgatga accacaccgg ctatgccacg ctggcggata tgcaggagta
    10501 tcagtttggc gcgttatatc tttctggtga cgagctgaaa aaaacgctgg gtgaacgctg
    10561 gagtgactgg aaacctgccg ccgggcaaac ctggcatagc tttaacgatt acattaattt
    10621 cagcgacaaa acaggctggg ataaatggtg gggaaaaaac tggatcagaa cggatatcgg
    10681 cgattacgac aatcctggat ttgacgatct cactatgtcg ctggcctttt tgccggatat
    10741 caaaaccgaa tcaacgaccg cttctggtct gccggtgttc tataaaaaca aaacggatac
    10801 ccacgctaaa gtcatcgacg gctttacccc tcgcgattac ttaacccact ggttaagtca
    10861 gtgggtccgc gactatggga ttgatggttt tcgagtcgat accgccaaac atgttgagtt
    10921 gcccgcctgg cagcaactga aaaccgaagc cagcgccgcg cttcgcgaat ggaaaaaagc
    10981 taaccccgac aaagcattag atgacaaacc tttctggatg accggtgaag cctggggcca
    11041 cggcgtgatg caaagtgact actatcgcca cggcttcgat gcgatgatca atttcgatta
    11101 tcaggagcag gcggcgaaag cagtcgattg tctggcgcag atggatacga cctggcagca
    11161 aatggcggag aaattgcagg gtttcaacgt gttgagctac ctctcgtcgc atgatacccg
    11221 cctgttccgt gaagggggcg acaaagcagc agagttatta ttattagcgc caggcgcggt
    11281 acaaatcttt tatggtgatg aatcctcgcg tccgttcggt cctacaggtt ctgatccgct
    11341 gcaaggtaca cgttcggata tgaactggca ggatgttagc ggtaaatctg ccgccagcgt
    11401 cgcgcactgg cagaaaatca gccagttccg cgcccgccat cccgcaattg gcgcgggcaa
    11461 acaaacgaca ctttcgctga agcagggcta cggctttgtt cgtgagcatg gcgacgataa
    11521 agtgctggtc atctgggctg ggcaacagtg acttttccgg cttcccgttc gtcagtacct
    11581 cgggaagccg ccaaccagga taaaatgtca gccctaatca gcgttgcagg ataaagcacc
    11641 actcactctt caacagaccg atttgcaccc cagcaaatgt agcgttattg ttaccttcct
    11701 tgctacagag ttcgacagat atcccgctat gacattctcc ctttttggtg acaaatttac
    11761 ccgccactcc ggcattacgc tgctgatgga agatctgaac gacggtttac gcacgcctgg
    11821 cgcgattatg ctcggcggcg gtaatccggc gcagatcccg gaaatgcagg actacttcca
    11881 gacgctactg accgacatgc tggaaagtgg caaagcgact gatgcactgt gcaattacga
    11941 cggtccacag gggaaaacgg agctactcac actgcttgcc ggaatgctgc acgagaagtt
    12001 gggttgggat atcgaaccac agaatattgc actaacaaac ggcagccaga gcgcgttttt
    12061 ctacttattt aacctgtttg ccggacgccg tgccgatggt cgggtcaaaa aagtgctgtt
    12121 cccgctggca ccggaataca ttggctacgc tgacgccgga ctggaagaag atctgtttgt
    12181 ctctgcgcgt ccgaatattg aactgctgcc tgaaggccag tttaaatacc acgtcgattt
    12241 tgagcatctg catattggcg aagaaaccgg gatgatttgc gtctcccggc cgacgaatcc
    12301 aacaggcaat gtgattactg acgaagagtt gctgaagctt gacgcgctgg cgaatcaaca
    12361 cggcattccg ctggtgattg ataacgctta tggtgtcccg ttcccgggta tcatcttcag
    12421 tgaagcgcgc ccgctatgga atccgaatat cgtgctgtgc atgagtcttt ccaagctggg
    12481 tctacctggc tcccgctgcg gcattatcat cgccaatgaa aaaatcatca ccgccatcac
    12541 caatatgaac ggcattatca gcctggcacc tggcggtatt ggtccggcga tgatgtgtga
    12601 aatgattaag cgtaacgatc tgctgcgcct gtctgaaaca gtcatcaaac cgttttacta
    12661 ccagcgtgtt caggaaacta tcgccatcat tcgccgctat ttaccggaaa atcgctgcct
    12721 gattcataaa ccggaaggag ccattttcct ctggctatgg tttaaggatt tgcccattac
    12781 gaccgagcag ctctatcagc gcctgaaagc acgcggcgtg ctgatggtgc cggggcacaa
    12841 cttcttccca gggctggata aaccgtggcc gcatacgcat caatgtatgc gcatgaacta
    12901 cgtaccagag ccggagaaaa ttgaggcggg ggtgaagatt ctggcggaag agatagaaag
    12961 agcctgggct gaaagtcact aaaaattgcc tgatgcgcta cgcttatcag gcctacatga
    13021 tttctgcaat atattgaatt tggaagaatt tgtaggccgg ataaggcgtt tacgccgcat
    13081 ctggcataaa caaagcgcac tattccggcg tttagaaacg ccggaaaacg tttcaaacag
    13141 gctgctgccg tagccgatgc cgctgcactt tctcgacatc catgcactgc aacgcctttg
    13201 tcgggcaggc ttcaacacag gccggtcccg tctcccgatg ccagcacaga tcgcacttaa
    13261 tcgccctcgc ctttttgcgc gaagaaacga cctccatcgc accaaacggg caagccagca
    13321 tacagctttt acagccaatg caacgtgatt gttcaacgaa aatatgccca tgttcgcggc
    13381 ttatcgcgtc aacagggcag acattcgcgc acggtgcatc ttcacactga tgacaggcta
    13441 ctgccgtggt ccagctgtgg tctttaataa cacgaatacg ggaaataaac tcgtctggtg
    13501 acaacgcagc gcaatcctga ttctcctgat gcgacactgc gcaagccact tcacaggtac
    13561 ggcaaccgat acatttcgtc gcatccgcaa taataaaccg gttcatgatc actcctttgc
    13621 actaaaacag cagagtgcca gagagcacgt ccctactgct ttgatccaac gaaggaaatg
    13681 ttaaagtttt ggcacttgtc caaagcgtta tgtgatcgcg cccaggtcat ccctgacagt
    13741 aaatcccagt tcattagaaa tagcctgcgc ggtttcacgc agtggtttca ggagattttt
    13801 ctcccccacc tgtttcagac gtgatgtcga aagcgaaatc gacacggcac tacgaattga
    13861 tatgttcctt gctgtaacgc cgcttccacg ctgctggcgt taaaccggtg tgtttgcgaa
    13921 aaatctgccg gaaatagcca acgtcattaa acccgcacaa cgccgccact tccgtgagcg
    13981 atcgcgcttc gctaattaac agcttttcgg cttcgcgcac ccgctggcga tggattgctt
    14041 ctgttaacgt cagatgaaac acccgccggt agacgcgccc aagataatcg gcattgcagt
    14101 acagctcgcg ggccagaagc gccgtagaaa gcgggcgatg atattgcgtg cggatcagtt
    14161 tctgcgcttt ccaggcaagg gtggtgcctg cgccatcggc ctgttcatca agacgcgcgt
    14221 cacgggtgag ctgctgcaaa atgagcagca caataaactc cagcgccgga gtgcgttgat
    14281 gcttttcctg ttcgcttaag aactggcgaa acagggccac cagcgcctgc ggatcgacaa
    14341 ccttcgtttg ttgcggcacg ctaagatgag taagccagcg agggtcgttg tccggcgctt
    14401 taacctcaaa atgcagccag taaaatttga gatcggcggg aaaatcctcc accccaacat
    14461 gttgtcggtt cggccagagt aataaactct ctcccgcatc gaccagaaat atccgttctt
    14521 cttcctgaat cgttaatcga cctttttcga caaagataat ttcccatgac tccagccgcc
    14581 ttgcaggatg cctccctacg ccacgggaaa taaataatcc accattttgc actttaatcg
    14641 gtaacgtcat ggataattcg agcattgata tgtaatttcc cgcttattca ttaatgatta
    14701 aacacattac cttttgatta ttaatcaaaa acattaattt cacctgatcc atttcacact
    14761 tttaaattca ggtcggaatc gtcacctttt tttactcttt tctcgccttg ttgatattcc
    14821 cgttcacggc agaattaatc aggtcatttc aataaaacta aaaattgctc cctattcacg
    14881 aggaatatct atgacctcta caccgattac taccgctgat atcgctaaag gtaaaattga
    14941 cgatgcgtta tctgtacggg aaaaaatagg ctacggcctg ggtgacgcag gcggcactgt
    15001 aataacttgc ctgatcatga attttctcac ctttttctac accgacgttt ttggattaac
    15061 tccggcgctg gttggcacgc tgtttattgc actgcgcgtg tttgatgcca tctccgaccc
    15121 ggtgatgggc gtcattgccg accggacgca aagtcgctgg gggcgctttc gtccgtggca
    15181 gctatggatt gccattccca tcggcattat cggcatcctg acgttcaccg tgccagatgc
    15241 cagcatggga gtaaaaatcg cctgggcgtt cggtacttac ctgctccttt cagtcggtta
    15301 taccgccatc aacgtaccgt actgcgcgct gatcaacacc atgaccaccc gccacaatga
    15361 agtgatctcc tgccagtcct ggcgattcgt tctctgcggc gtagcgggat ttctggtttc
    15421 ggtaggctta ccgtggatgg tagatctctt cggtcagggc aacgctgcac gcggctatca
    15481 actgggcgtc ggggtattgt gcgccattgc cgtggtgatg ttcctgtgct gtttcttctg
    15541 ggttcgtgaa cgggtgccgc tctccacaat ggggaaattt accctgcgcg aacatcttgc
    15601 cgggctgcgg aacaacgacc aactgctgct gatgctggtc atgtctttcc tgctgattaa
    15661 cgtctttaac attcgcggcg gtgggtatat gtacttcatt acctacgtct tacaaggcag
    15721 tacgggctac acgtcgctgt tcttcaccat ggtcaccttc gcctccatta tcggctcggt
    15781 gattgtcagc ccgttaacgc ggcgtttcga taccgtcaaa atttactact acaccaacca
    15841 gctcctcgct gcgctggcgg tgttgatgtg gttcctgccc tccggcccgg cttatcaaac
    15901 gctgtggctg gcggtgatcc tcggtaatgg cgtaattctt ggcttcacat tgccactgca
    15961 cttctcattg atggcctttg ccgatgacta cggcgagtgg aaaacccgcg tacgttcttc
    16021 cggcatgaac ttcgccttca atctgttttt catcaagctg gcctgggcct ccagcgccgg
    16081 gatcatcagc ctgctgttta tttttgtcgc ctaccagcct ggcgtggaaa accagaccgc
    16141 cagttcgctt ggcgggatcg cggcgatgga aacattactg cctgcgctat tccacctgct
    16201 gctggcaggg gcgatccgct tttgcaaact caataatcct atgatgtcac gcatagctac
    16261 cgacctgcgt cagcgtcatg tacagcctta aggagcacaa gatgaacatt tcggaagtcg
    16321 atctgcataa actgacggtc agcgatccgt tcctcggtca gtaccaacaa ctggtccgcg
    16381 acgtggtgat tccttatcag tgggatgctt tgaacgatcg tatcccagaa gcggaaccca
    16441 gccatgcgat tgaaaacttt cgcattgccg ccggacttca ggagggtgaa ttttacggga
    16501 tggtgtttca ggacagcgac gtcgccaaat ggctggaagc ggtagcctgg tcgctgtgcc
    16561 agaagccgga cgccgaactg gaaaaaaccg ccgacgaggt aatcgaactg atcgcctccg
    16621 cccaatgcga agacggctat ctcaatactt actttacggt aaaagcaccc gaagaacgct
    16681 ggagcaatct ggcggagtgt catgaacttt actgcgcagg tcatctgatt gaagccggag
    16741 tcgccttctt ccaggccacg ggcaagcggc gcttgctgga agtggtttgc cgcctggccg
    16801 atcatatcga cagcgtattt ggtccagatg aaagtaagtt acacggttat cctggtcacc
    16861 cggaaattga actggcacta atgcgcctgt atgaagtgac tgaagagccg cgctacctgg
    16921 cactgacgaa ctattttgtc gaacagcgtg gtgcgcaacc gcactattac gaccaggaat
    16981 atgaaaagcg cgggcagaca tcgcactggc acacctacgg cccggcgtgg atggtgaaag
    17041 acaaagccta cagccaggca catttgcccc ttgcgcaaca gcaaaccgcc atcggtcacg
    17101 cggtacgttt tgtctatctg atgaccggcg tcgcgcatct cgcgcgttta agtcacgatg
    17161 acagcaagcg tcaggactgc ctgcggctgt ggaacaatat ggcccagcgt cagttatata
    17221 ttaccggcgg cattggctca cagagcagcg gcgaagcgtt cagcagcgat tacgatctgc
    17281 cgaatgacac ggtttacgcc gaaagttgtg cttccatcgg cctgatgatg ttcgcccgac
    17341 gaatgctgga aatggaaggc gacagtcaat atgccgatgt gatggagcgc gcgctgtaca
    17401 acactgtgct cggtggcatg gcactggatg gcaaacattt cttctatgtg aatccgctgg
    17461 aagtacatcc aaaatcgctg aaattcaacc atatctacga tcacgttaaa ccgatccgcc
    17521 agcgttggtt tggctgcgct tgttgtccgc caaatatctc ccgcgtgctg acctcgattg
    17581 gtcattatct ctacacgccg cgtgaagatg cgttgtatat caacatctac gcaggaaaca
    17641 gcatggaagt gccggtagaa aatggcacgc tgcgcctgcg ggttagcggg aactatccgt
    17701 ggcaggagca ggtgacgatt gtggtcgaat cgccccagcc ggtacgtcat acgctggctt
    17761 tacgtctgcc ggactggtgc acacagccgc agatcatatt gaatggggaa gaggtcgagc
    17821 aggatattcg taaagggtat ttgcacatta cccgcgaatg gcaggagggc gatacgctga
    17881 atctgacttt gccgatgccg gtacgccgcg tttacggtaa cccgctggtg cgtcacgtcg
    17941 ccggaaaagt ggcgattcag cgcggcccgc tggtgtattg cctggaacag gccgacaacg
    18001 gcgagtcact gcataatctg tggctgccca ccgatgcgcc atttacgaca tttgaaggca
    18061 agggattgtt tagccataag atcttaatcc aggcaccggg ttaccggtat gaacagagca
    18121 atccagagca gcaaccgctg tggcattacg acagcgcgcc agccaaacgc cagacgcaaa
    18181 ctctgacctt tatcccgtgg tttagctggg ccaaccgggg tgaaggcgaa atgcggatct
    18241 gggtgaatga ggaaaagcat tgccatccgt aggttggata aggcactcgc gccgcatcca
    18301 acgtaaggag cacattgcct gatgcgacgc ttaacacgtc ttatcaggca tacaagtcgg
    18361 tgcgagagcg taggccagcc gcaccgacat ccaccggtga attaaaaacg ccagctcacg
    18421 ccggtgacaa aggtaaaact ctcattacga tcaatcatcg ggctgtcggt aatctcttcc
    18481 ggcagctcgc tgtatcctgc gctcgccatc aatacgacgt gctctcctat cgggtatttt
    18541 gccgtcaggc tgacataggg cacccaggca tcctgcgcgg aataacttgc cagaccgcta
    18601 cgacgggact cactctctga aatgccatag taatagtcac tgaaattctc gtcataatag
    18661 agaacgccca gcgcaggtgt cagcgacaga cgacctatct gcattttgtg gaataccgat
    18721 agctccccca cccagccgtt gctgttatcc agaacgtccg cagctgcaga ggctttcacg
    18781 gttccccaac gctcgtggtg ataccaggca acccccgcca tagccgtact atcacgctta
    18841 tcaagctgtt gcatggcata atcgtcatta tcggcagggt caaattccag cggcatccag
    18901 gatgcggtca ggctaagttc atttttttca ctttgcgaca gaataaaacc taacgtggtc
    18961 tgacgaacat aaaaagaatc accttcataa ctaatcaggg gaattgcctt tgtattttca
    19021 ttataaccgc gataaggcga ttcattataa gccgcaccgg caccaatgga taattcagaa
    19081 gcagttgcgc ttgccataaa aggcaacgca aataacgcca ctatattgcg gttaattaac
    19141 atgtcaggta ttccatttta aaataagata aaaaagagtc ggcatagtaa tg
//
LOCUS       DAZFKF010000097        19141 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFKF010000097 DAZFKF010000000
VERSION     DAZFKF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.097"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..433
                     /gene="eilA"
                     /locus_tag="ACHKA1_003994"
     CDS             <1..433
                     /gene="eilA"
                     /locus_tag="ACHKA1_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /protein_id="HFO5681571.1"
                     /translation="NAISFALNLNSQNTCNNPIITSILAMFMALKGHNDKAKSLLLKL
                     EPEHGLDYTCVNSLYTKFLIYGASIKNDIMKLLANINTNKINGVILPLIYSVYGKKEY
                     EKRWQQLIKESDLWSNVLLHDPRLLSVKNEFNTIGVMRSSA"
     gene            complement(483..8108)
                     /gene="iatC"
                     /locus_tag="ACHKA1_003995"
     CDS             complement(483..8108)
                     /gene="iatC"
                     /locus_tag="ACHKA1_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin IatC"
                     /protein_id="HFO5681572.1"
                     /translation="MAGKAHGNGDRRGDNTICGLGDRLRRLTAGICLITQTIFPVMAA
                     APTHINHAHSDTTTSLILPNVKTIPYTLGALESPSTVAARFGITVDELRRLNQFRTFA
                     RGFDNVRQGDEIDVPLINSNSPEARNLKAMQMERDGKDPQMQVAEMAQQSGTLLARDM
                     DSEQAASMARGWVASSASAQATDWLSRWGTARVSLGVDEDFSLKSSSFEFLHPWYETP
                     DNLVFSQHTLHRTDDRTQTNHGIGWRYFTPSWMSGVNMFIDYDLTRYHTRTGMGVEYW
                     RDYLKLSGNGYLRLSNWRSAPELDHDYEARPANGWDLRAEGWLPAWPQLGGKLVYEQY
                     YGDEVALFGKDERQNDPHAITAGLSYTPVPLISFSAEQRQGKQGENDTRIGMELTLQP
                     GHSLQKQLDPAEVAARRSLVGSRYDLVDRNNNIVLEYRKKELVRLTLTDPLKGKPGEV
                     KSLVSSLQTKYALKGYDIEAASLQSAGGKVAVSGKDIQVTIPPYRFTAMPETDNTYPI
                     AVTAEDSKGNFSRREESMVVVEEPTLSLADSTLSVDLQILLADGKSTSTLTYTARDSS
                     GKPIPGMTLKTQAKGLQDFALSEWKDNGNGTYTQIVTAGKTSGALSLMPQFNGDDIAK
                     TPALIAIVANTASRADSTIETDQDNYVAGKPIVVKVTLRDDNDNGVTGRKELLKQTVK
                     VDNTKADAVSAWTEESEGIYKASYTAHLIGDKLTAQLTMPGWQTKHSDAFSIAGDKDT
                     AKIAAMQITANNAVARRDHNTVAVTVRDVHQNLLQGQNVTFTVVNGTAVFADPNGGIV
                     TTDKDGIASVNLASDQAVNSLIKAEINGSSQSVEVSFITGDISQLTSTIKTDDVSYTA
                     GGKIKVSVTLMDEQKNLVKGMASLLAGSGVVEVSGTDKNETGNWSEESDGVYTTTRTA
                     KIAGDRHYATLKLSTWSSAQQSDAYAIRESGAVLAYSSIVTDKTTYTAGGVIKVTVTL
                     KDSYENLVGGQRDAINQAIQLPNTKAESIAWNEDQKGIYTATYTALLPGTGLKAQLQM
                     SDWASALTSNDYSISGDAASAQIVAMQVTTGNPDVLANGSDRHTVNVRVEDQFGNVLP
                     EQTVTFTVTKGAAVFANAGQSADIRTDAHGMAEVDLSSTVADASTVEAKVNQSSDSKT
                     VNFVADVRTAQIADLVVIKDGSVADGSTANTLRVRVSDAFGNALDGQTVSVLADNGAT
                     VAPTVITEPDGTVEISVTSQTAGVSAVTASINNSSLSRNVTFVADVRTAKIADLVVMQ
                     DGSVADGTTANTLRARVTDAFGNALAGQTVSVTAGNSATVASTVTTKPDGTVEISVTS
                     QTAGVSTVTASINSSSQSRDVTFVADVRTAKIADLVVTRDNSVADASTANTLQVKVTD
                     AFGNTLAGQTVSVTAGNGATVAPTVITEPDGTVEISVTSQTAGISTVTASINSSSLSR
                     DVMFIADIRTAQIADLVVIKDGSEADGATANTLRARVTDAFGNTLAGQTVSVMADNGA
                     TVASTVTTGQDGTVEISVTSQTAGTSVVTTSINSSNASRNVTFIADVRTAQIADLVVI
                     KDGSEADGATANTLRARVTDAFGNALAGQTVSVSADNSAMVTPTVTTQPDGTVEISVT
                     SQTAGISTVTASINSSSQSRDVTFIADVRTAQIASLEVTQDNAVADGAMVDMLRARVT
                     DAFGNALAGQTVSVMADNGAAVASTMTTKPDGTVEISVTSQTAGISVVTASINSSSQS
                     RDVTFIADIRTAQIADLVVIKDGSVADGAMANTLWVRVTDAFGNALAGQTVSVLADNG
                     ATTAPTVTTQPDGTVEISVTSQTAGISTVTATINNHSLSQNVTFVPGDASQLTSTVET
                     NKSNYTVGETITITVTLRDAFDNLVTGAASQLAANSVLTVDGTDPSETGSWVESGGVY
                     TTTRMATIAGTDQHANLQLQTWSDGVTSDRYDIQSGSPAQATSTIATDKNAYTAGETI
                     TVAVTLKDAHGNLVEGGESLLSGDNVTVEGAVRSGEWSETAGVYTATWSAQMAGDSHH
                     ATLTLPEWGNSKQSESYSIHSGAPVQANSAIRTDKSAYIAGEPLTVTITLRDEFGNPA
                     LGLTSEVIESYIDSFAVGGATPDSMQWVEQNNGEYTIVWTAWVAEENLVASLKLKTWA
                     TEIKSSLYGIQPGAAAQTQSTIVTDKTKYIAGDSITVTVVLKDAQGNFITDGVAQLNE
                     ENVQVRNADSIQGNNWVYNGNGQYQRQYMAHFAEANLNAQLKMAGWSDANYSKNYTIN
                     RGEVSMLRSQLRIREVLVVAGADIPVSVLLSDEFGNPVNDGLDLLTDDAVYLQNVEKK
                     HWSSWTFVGDGRYERTYMAYKEGENLNSYLHINGWYVGGQPSYTILPFVEVESLSVNG
                     AKFRAADGFPKTGFDGAKFTLILTHNMKNTDYNWTSGIQGIQVDSNGMVTLEFIINKE
                     VTITGTPKSNKGNKVTYKFSLQKWFIPQGIIQESWSEMNSYCIGNGYILPSSTDLVGS
                     STSGAVPRKVGSLWGEYGNLTSYDGIFRAEHYWLDSGMIFYPGDGHLSIAPRSSPLCM
                     KTF"
     gene            complement(8310..9494)
                     /gene="nepI"
                     /locus_tag="ACHKA1_003996"
     CDS             complement(8310..9494)
                     /gene="nepI"
                     /locus_tag="ACHKA1_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO5681573.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLCGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MK"
     gene            9728..10081
                     /locus_tag="ACHKA1_003997"
     CDS             9728..10081
                     /locus_tag="ACHKA1_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5681574.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            10065..10388
                     /locus_tag="ACHKA1_003998"
     CDS             10065..10388
                     /locus_tag="ACHKA1_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5681575.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LDVLL"
     gene            complement(10504..10956)
                     /gene="yicN"
                     /locus_tag="ACHKA1_003999"
     CDS             complement(10504..10956)
                     /gene="yicN"
                     /locus_tag="ACHKA1_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO5681576.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(11009..12343)
                     /gene="adeQ"
                     /locus_tag="ACHKA1_004000"
                     /gene_synonym="yicO"
     CDS             complement(11009..12343)
                     /gene="adeQ"
                     /locus_tag="ACHKA1_004000"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO5681577.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            12518..14284
                     /gene="adeD"
                     /locus_tag="ACHKA1_004001"
     CDS             12518..14284
                     /gene="adeD"
                     /locus_tag="ACHKA1_004001"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO5681578.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYTDAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(14332..15723)
                     /gene="uhpT"
                     /locus_tag="ACHKA1_004002"
     CDS             complement(14332..15723)
                     /gene="uhpT"
                     /locus_tag="ACHKA1_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO5681579.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(15861..17180)
                     /gene="uhpC"
                     /locus_tag="ACHKA1_004003"
     CDS             complement(15861..17180)
                     /gene="uhpC"
                     /locus_tag="ACHKA1_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO5681580.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            complement(17190..18692)
                     /gene="uhpB"
                     /locus_tag="ACHKA1_004004"
     CDS             complement(17190..18692)
                     /gene="uhpB"
                     /locus_tag="ACHKA1_004004"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO5681581.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(18692..>19141)
                     /gene="uhpA"
                     /locus_tag="ACHKA1_004005"
     CDS             complement(18692..>19141)
                     /gene="uhpA"
                     /locus_tag="ACHKA1_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO5681582.1"
                     /translation="VQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQAL
                     NAGARGFLSKRCSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKL
                     AQGMAVKEIAAELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
BASE COUNT         4793 a         5340 c         4613 g         4395 t
ORIGIN      
        1 taacgcgata tcttttgcat taaatcttaa cagtcaaaac acctgtaaca atccaattat
       61 tacatctatt ctggctatgt tcatggcact gaaaggacat aatgacaaag ccaaaagttt
      121 attgcttaaa cttgaacctg aacatggtct tgattacacc tgcgttaact cgctttatac
      181 gaagtttctg atctatggcg catctataaa aaatgacatt atgaagttac tggcaaatat
      241 taacaccaac aaaatcaatg gtgttatttt gccgttaatt tacagtgttt atggtaaaaa
      301 agaatatgaa aagcgctggc aacaattaat taaagagagt gatttatggt caaatgtgtt
      361 gcttcatgac ccccgtttgc tcagtgtgaa aaatgaattt aatactattg gtgtgatgcg
      421 gagttctgcg taagagcgaa gataaattgc cctgctgatg cagggcaata acataaataa
      481 tatcagaacg ttttcataca cagtggggat gaacgtggcg caatcgagag atgaccatct
      541 cctggataaa atatcatacc gctgtcgagc caataatgtt cagcgcgaaa tattccgtca
      601 tacgaagtca gattaccata ttcaccccat aacgaaccta cttttcgcgg gacagcaccg
      661 ctagttgaac tcccaactaa atccgtacta gacggtagta tataaccatt accaatacaa
      721 tatgaattca tctcactcca tgattcctga attatccctt gaggtataaa ccatttctgc
      781 agtgagaatt tatatgtaac tttattccct ttattgcttt tcggcgttcc tgtaatagta
      841 acctctttat tgataatgaa ttctaaagtc accataccat tactatcgac ctgaataccc
      901 tgtatgccac tggtccaatt ataatctgtg tttttcatat tgtgggttaa tatcagtgta
      961 aatttagcac catcaaaccc tgttttggga aaaccatctg ccgccctgaa ttttgcacca
     1021 ttgacactaa gcgactcgac ttccacaaaa ggaaggatgg tataagacgg ttgcccgcct
     1081 acataccaac cattaatatg tagataagag tttaagtttt ctccttcttt gtaagccata
     1141 taggtacgct catatctgcc atcacctaca aatgtccaac tggaccaatg tttcttttcc
     1201 acattttgta agtacactgc atcatctgtc agcaaatcca gaccgtcatt cacaggatta
     1261 ccaaattcat cagataataa aacactaaca ggtatatccg ccccagctac cactaaaact
     1321 tcgcgtatac gaagctgtga acgaagcata cttacttcac ctctgttaat agtgtagttt
     1381 tttgaataat tggcatcgct ccaaccagcc atcttcagtt gcgcattcaa gttcgcttcc
     1441 gcaaaatgcg ccatatactg tctttgatat tgcccattgc cgttgtaaac ccaattattg
     1501 ccttgaatag aatcagcgtt cctgacttgt acgttttcct cattaagctg cgcgacacca
     1561 tcagtaataa agttaccctg ggcatcttta agaacaacgg tcaccgttat gctatcccca
     1621 gcgatatatt tcgttttgtc cgtgacaatc gtggattgag tttgtgccgc cgcacctggt
     1681 tgtattccgt ataatgagga cttaatctcc gttgcccatg tttttaattt cagactggca
     1741 accagatttt cctcggcaac ccatgctgtc cagacaatgg tatattcacc attattctgc
     1801 tcgacccact gcatagaatc gggagttgcg ccacctaccg cgaaactatc gatataagac
     1861 tcgatgactt ctgatgtcag ccctaaagca gggttaccaa actcatcccg caacgttatt
     1921 gtgacagtta atggctcccc ggcgatatat gccgatttat ctgtcctgat agcagaattt
     1981 gcctgcaccg gagcaccact gtggatggaa taactctctg attgtttact gttgccccac
     2041 tcgggcaacg tcagcgtcgc gtggtgagag tctcccgcca tttgcgccga ccatgtagca
     2101 gtataaacac cggcagtttc agaccactct cctgaacgta cggctccttc cactgttacg
     2161 ttatcaccag acagtaagga ctcgccccct tcaaccaggt taccgtgcgc atctttcagc
     2221 gtcacggcga cggttatagt ctcgccagcg gtgtatgcgt ttttatccgt ggcgatagtt
     2281 gatgtggctt gcgccggaga accggactgt atgtcatagc gatctgacgt tacaccatca
     2341 ctccacgtct gcagttgcag gttagcgtgc tgatcggtac cggcaatcgt cgccattcgg
     2401 gttgtggtat acacgccacc tgattcaacc cagcttcccg tttctgacgg gtcggtgcca
     2461 tccaccgtca gcacactatt agcggctaac tgtgaagctg caccggttac cagattatcg
     2521 aacgcatccc ggagcgttac cgtgatggtg atggtctctc caaccgtgta gttagactta
     2581 tttgtttcaa cggttgaagt gagctgcgat gcgtcacccg gtacaaaggt gacgttctgg
     2641 ctcaggctat gattgttgat ggttgcagtg actgtactga tccccgccgt ctggctggtg
     2701 acactgattt ccaccgtgcc gtccggctgc gtggtgaccg tcggagctgt cgtggcaccg
     2761 ttgtccgcca acacgctgac cgtctgcccg gcaagcgcat taccgaacgc atccgtgacc
     2821 ctcacccaca gcgtgttcgc cattgcgccg tctgccactg agccgtcctt aatgaccacc
     2881 aggtccgcga tctgcgccgt cctgatatcg gcgataaacg tcacatcccg gctttgactg
     2941 ctgctgttga tgctggcggt gaccacactg attcctgccg tctggctggt gacactgatc
     3001 tccaccgtgc cgtccggctt tgtggtcatc gtcgaagcga ccgctgcacc gttgtccgcc
     3061 atcacgctga ccgtctgccc ggcaagcgca ttaccaaacg catcggtgac cctcgcccgc
     3121 agcatatcca ccattgcgcc gtcagccacg gcgttatcct gcgtcacctc cagactcgcg
     3181 atctgcgccg ttctgacatc ggcgataaac gtcacatccc ggctctggct gctgctgttg
     3241 atgctggcag tgactgtact gatccccgcc gtctgactgg tgacactgat ctccaccgtg
     3301 ccatccggct gcgtggtcac cgtcggagtg accattgcgc tgttgtctgc cgatacgctg
     3361 accgtctgcc cggcaagcgc attgccgaac gcatcggtca ccctcgcccg cagcgtattc
     3421 gccgtcgcac cgtccgcctc tgaaccatcc ttaatgacca ccagatccgc gatctgcgcc
     3481 gtcctgacat ccgcaataaa cgtcacgttc ctgctcgcgt tgctgctgtt gatgctggtg
     3541 gtgaccacac tggtgcccgc cgtctggctg gtgactgaga tctccaccgt gccgtcctgc
     3601 cctgtggtga ccgtcgaagc gaccgttgcg ccgttgtccg ccatcacgct gaccgtctgc
     3661 ccggcaagcg tattaccgaa cgcatcggtc accctcgccc gcagcgtgtt cgccgtcgca
     3721 ccgtccgcct ctgaaccatc cttaatgacc accagatccg cgatctgcgc cgtcctgata
     3781 tcggcgataa acatcacgtc ccggctcagg ctgctgctgt tgatgctggc agtgactgta
     3841 ctgatccccg ccgtctggct ggtgacactg atttccaccg tgccgtccgg ctctgtaatc
     3901 accgtcgggg caaccgttgc gccgttgcct gctgtcacac tgaccgtctg cccggcaagc
     3961 gtattaccga atgcatcggt gaccttcacc tgcagcgtat tcgccgtcga agcgtctgcc
     4021 actgagttat cccgggtgac caccaggtcc gcgatcttcg ccgtcctgac atcagcgaca
     4081 aacgtcacat cccggctctg actgctgctg ttgatgctgg cggtgacagt actgactccc
     4141 gccgtctggc tggtgacact gatctccacc gtgccgtccg gctttgtggt cactgtcgaa
     4201 gcgaccgttg cgctgttgcc tgcagtcacg ctgaccgtct gcccggcaag cgcattgccg
     4261 aacgcatcgg tcaccctcgc ccgcagcgtg ttcgccgtcg tgccatctgc cactgagccg
     4321 tcctgcatca ccaccagatc agcgatcttc gccgtcctga catcggcaac aaacgtcacg
     4381 ttccggctca ggctgctgtt gttgatactg gcggtgaccg cactgactcc cgccgtctgg
     4441 ctggtaacag agatctccac cgtcccgtcc ggctcggtga taaccgtcgg agcgaccgtt
     4501 gcgccattgt ccgccaacac gctgaccgtc tgcccgtcca gcgcattacc gaacgcatcc
     4561 gacaccctca cccgcagcgt attcgccgtc gaaccgtctg ccactgagcc atccttaatg
     4621 accaccagat cagcgatctg cgccgtccta acatcagcaa caaagttcac cgttttacta
     4681 tcgctgcttt gattgacctt agcctcaaca gtgctggcat ccgctacggt actgctcaga
     4741 tcaacctctg ccataccatg cgcatccgtc ctgatatctg cgctctgccc tgcattggca
     4801 aatacagccg cccccttcgt caccgtaaag gtgacggtct gctccggcag cacattgcca
     4861 aactgatctt ccacccggac attcaccgta tgtcgatcgc tgccattcgc cagcacatcc
     4921 gggttacccg ttgtcacttg catcgcgaca atttgtgcgc tggcggcatc gccactaata
     4981 ctgtaatcat ttgaagtcag agcgctcgcc cagtcggaca tctgtagctg tgcttttaaa
     5041 ccggtgccag ggagtaaggc ggtatacgtt gcggtataaa ttcctttttg atcttcattc
     5101 caggcgatgc tttccgcttt tgtatttggc agctgaatag cctggttgat agcgtctcgt
     5161 tgccccccga ccaggttttc atagctatcc ttgagtgtta ccgtaacttt tatcacgccg
     5221 cccgcggtgt aggtcgtttt gtcggtaacg atggaggaat aagccagcac agctccgctt
     5281 tcgcggatgg catacgcatc ggattgctgc gcgctactcc atgtcgatag tttcagcgtg
     5341 gcgtaatggc gatcgccagc aatcttcgcg gttcgggtgg tggtgtaaac gccgtcagac
     5401 tcttcgctcc agttacccgt ttcattttta tctgtaccgc tcacctcgac cacaccgcta
     5461 cccgccagca gcgaagccat ccctttcacc aggtttttct gctcatccat aagggtgacg
     5521 gacactttta tcttgccacc cgcagtgtat gacacatcgt ccgtcttgat ggtggaggtc
     5581 agctgcgaga tatcgccagt gataaagctc acttccacag actgactact gccattgatc
     5641 tcggccttga taagactgtt taccgcctgg tcactggcaa ggttaacgct ggctatcccg
     5701 tctttatctg tcgtcacaat accgccattc ggatcggcaa aaaccgccgt accattcacg
     5761 accgtgaagg taacattttg tccctgcagt aaattttgat gcacatccct gaccgtcaca
     5821 gcgacggtat tgtgatcgcg tctggcaact gcgttattcg cggtgatctg catcgcagcg
     5881 attttggcgg tatctttatc acccgcaata ctgaacgcat ccgaatgttt ggtttgccag
     5941 ccgggcatcg tcagctgggc cgttaatttg tcgccaatga gatgggccgt atagcttgct
     6001 ttgtaaattc cctcactttc ttccgtccag gcactaacgg catcagcctt cgtgttgtct
     6061 actttaacgg tttgtttgag aagttctttt cgtccggtaa ccccgttgtc gttatcatct
     6121 ctcagggtca ctttaacgac tatcggctta cccgcgacgt aattatcctg gtctgtctca
     6181 atcgtggagt cagcacgaga tgccgtattc gccacaatgg ctatcagcgc tggcgtctta
     6241 gcgatatcgt cgccgttaaa ttgcggcatc agcgataacg cgccagacgt cttcccggcg
     6301 gtaactattt gggtataggt tccgttaccg ttgtctttcc attcgcttaa ggcgaaatcc
     6361 tgcaaacctt tcgcctgtgt ttttaacgtc ataccgggga ttggcttgcc gctggaatct
     6421 cgcgcggtat aggtcagcgt tgaggttgat tttccatcag caagcaggat ttgcagatcg
     6481 acagaaagcg ttgaatcggc gaggcttagt gtgggttctt ccacgaccac catactttct
     6541 tcacgccggg agaaatttcc tttcgaatct tccgctgtaa ccgctatcgg gtaagtgttg
     6601 tcggtttcag gcattgccgt gaatcggtag ggaggaatag tgacctgaat atccttgccg
     6661 gaaacagcta cttttccgcc cgctgattgc agggaggcgg cttcaatgtc ataacctttc
     6721 agcgcatatt tggtttgtaa ggaagaaacc agcgatttaa cctcacccgg cttgcctttc
     6781 aacggatcgg tcagcgtcag ccgcaccagc tctttcttgc gatattcgag gacaatattg
     6841 ttattacgat cgaccaggtc atagcgactg cccacaaggc tgcgtcgtgc tgcaacttca
     6901 gccggatcga gctgtttttg cagtgaatga ccaggttgca acgtcaactc cataccaatt
     6961 cgggtgtcgt tttctccctg tttgccctgc cgttgctcgg cactaaagga gatcagagga
     7021 acaggggtgt aactcaaacc tgcggtaatc gcgtgaggat cattttgtcg ctcgtcttta
     7081 ccaaacagcg ccacttcatc gccataatat tgttcataga ccaacttgcc gcccagctgc
     7141 ggccaggccg gtaaccagcc ttccgcgcga agatcccacc cgttcgccgg acgtgcttca
     7201 tagtcatggt cgagttccgg tgcactacgc cagttactca ggcgcagata accgttgccg
     7261 ctcagtttca ggtaatcgcg ccagtattca acgcccatcc cggttcgggt gtgatagcgg
     7321 gtcaggtcat aatcgataaa catattgacg ccagacatcc agctcggggt gaaataacgc
     7381 cagccgatac cgtggttggt ttgagtgcga tcatccgtac gatgaagtgt gtgctggctg
     7441 aagaccaggt tatccggcgt ttcgtaccag ggatggagaa attcgaatga agagctttta
     7501 aggctgaaat cttcatccac accaagagat acccgggcag ttccccaacg actgagccag
     7561 tccgttgcct gcgcagatgc agaagaagcc acccagccac gcgccatact cgccgcctgt
     7621 tcgctgtcca tatcgcgagc taaaagtgtt ccgctttgct gcgccatttc cgcgacctgc
     7681 atttgcggat ctttgccgtc gcgctccatc tgcattgctt tcaggttgcg cgcttcaggg
     7741 ctattactgt taatgagggg cacatctatt tcatcgccct gacgcacatt gtcaaagccc
     7801 cgcgcaaagg tacgaaactg gttcaggcga cgtaactcat caacggtgat accaaagcgt
     7861 gccgcgaccg ttgatggcga ttccaatgcc cccagggtat atggaatagt tttaacgttc
     7921 ggcaggatca gggacgtggt agtatccgaa tgcgcatgat taatatgcgt aggggcagcg
     7981 gccataacag ggaatatagt ttgtgttatc aggcaaatac cggcggtcag acgacgtaaa
     8041 cgatccccca atccacatat ggtgttatcg ccccgacgat cgccattccc atgcgctttc
     8101 cctgccattt gtcatccctg tcgctatgcg ttcagattaa atgacctttc tttaacatga
     8161 gggaaaatga tgattcatga ttagcaaatg atgaatacag gcggaataat ggaaataaca
     8221 acaagataag aacaatacct gatcaagcgt tatcaggggg aagaacagga tagctttccc
     8281 gccagagaaa taaccctcct gtaaatacgt tacttcattt tcacctttgc ggtgactaat
     8341 aacgccgtca gcaacatcaa tgtgccggac aacatcaacg gcgaagtcag accaatatta
     8401 tccagcgcat aaccgccgat tgccgcgcca caggtattag caagctgaat aaccgccacc
     8461 tgaatagacc cggctttttc tgcctgatcg gccagtgagc gggtgatcca cgttgaccag
     8521 ccgacgggaa ccaatgcaaa agttagcccc cagataatcg ccacgccggt agcaacgatt
     8581 ttatcgcttc cacacaacgt cagcaccaac gcactcacag ccagtattaa cggcgcgcct
     8641 gctaaggcca gttttaccga acgtttaaga atgaacgacg aaagcgacgt accgataaag
     8701 ctggcgatac caaaacttaa cagcaccagc gttaagccat ccacaccgaa tcccgccagg
     8761 ttcatataca ctggacgaat atatgtgaag aaagcaaact gtccggcgaa agacatgaag
     8821 atggcgatca tccctgccat cacacccgga cgttgtaata agcggaaagt attttgtttc
     8881 tgatgcgagg gttcgcctgg cagtgaaggc aatgatttga taatccagaa aatacacagc
     8941 acgcccatca ccgccgccgc attaaagaca ttgcgccaac cgataagctc gcctaaaaaa
     9001 ctgcccaacg gcgcggcaat caccagcgca atagaaaccg cgccgaagat caccgacagc
     9061 gccttcggca ccgtacgcgg cggtaccaga cgcatggtca gcgacgccga catcgcccag
     9121 aacccgccca gcgccagccc cagacaggca cgaccgatta aaagcaaact gaatgagtta
     9181 gcaaaggaaa ccagcaaaca ggagagcgtc agcaaaacgg caaataaaat aacaacgtag
     9241 cggcggtcag tggcctgaat tgtctgggta ataaacaaac tggcaaacat tgccacaaag
     9301 gcggtcacgg tcaccgattg cccggcaacc ccttccgaaa tgcctaaatc ctgggccatt
     9361 ggcgtcaaca aactgacggg caaaaactca acgataatca gacaggcgac acaaaacgcc
     9421 accgagaaaa cggctgacca gttcggtcgg gtgatggcat ccgcgccgcg gttttcagca
     9481 ataaattcac tcattgtgtt acccgtggca tgtttcaggg gaaaagccga ccactttaac
     9541 attgaaatgt gtgacgcatt taacgttttt gcaactttta cggtgttgtg ggaaaagttc
     9601 gaggctgctc gaagaaacaa aaatcatagg gcaagctgat atgcattttc gcgattgggt
     9661 tgggaataag ggataaagta ttgcacttgg tgatatactt taataacagg acctcaacga
     9721 tatgcgcgtg tttaaaacca aatggtttgc aagggaagcc aggtctcatg caataacaga
     9781 tgaagagttg tgccgggcca ttttagaaac ggagcaaggt aaagctgatg cacttggtgg
     9841 tggcgttttt aaaaagcgcc ttcaccaaaa tcgtgagcgt gcaattattc tggcaaaagg
     9901 gggaagcaat tggttttaca cgtttttata tgcaaaacag gatatgtcga acatcaatag
     9961 tcaggaactg gctgggtttc gtgaacttgc aaagcattat gccttcctga cgaaagccca
    10021 gctaacagca atgattaaca ctaaagaact gacagagatc tgctatgact gcaaaaacta
    10081 aatttaaaag tcctgcattt gaggctattc acagcgcagc ggctggattg tccagcgtcg
    10141 atgctatttc tgccgaaaca atgcgtactt ttgacaaagc ctgcctgacc agcgtgcaag
    10201 acttacaacc tgttgagata aaagcactgc gggaaaaatt aaaagtgagt cagcccgtct
    10261 tcgctcacta tctcaatacc agcgtttcaa ctgtacaaaa atgggaaagt ggcgctaaac
    10321 gccccaatgg catttcatta aaactgctca gcattgtgca aaaacacggg ctggatgtgc
    10381 tgctgtaaat agaacgtaga taatgcgttc acctactgtc agttccgata ccggacgcgg
    10441 cgtgaacgcc ttatccactc gacaggtcag gcacggtctg taggctgata agacgaagcg
    10501 gttttaatgc agccagtgaa cgccctcggc aggctgaaag aatgcgttat agcgcatctg
    10561 aaaagcatta atatcctgca tttcaggttc agtttcgcgc agaaaaccca gcgccagcct
    10621 gacctgagca tcggtaatcg gcgcggcaag gcgggctttt tgtaaaatcc gatgccattg
    10681 cagcaggttc tgttcgctac catcgacaat gacaatttgc cagatgagca acacctgcgc
    10741 ggcgttttgc aggtgcgact catccggacg atgcaaataa cgtaaaaatt cgtcaccagc
    10801 tgactttccc atttgatcga ccatcgactc cacgggtacg acatcgatat tcagccgatc
    10861 gctgagacgg cgaatcgctt ttctggcccc ggatgtcagc acccgaaaac caaccacaaa
    10921 cactaccgcc agcgtggcga gcattatcca gatcatgagt tctcctgcgt aatcagaact
    10981 catgatatct ggaaaccctc gccagttact aatccaccag aataatcttc agtgcaaaca
    11041 gagctgcgac caccaccaca cacaggttca gatcgcgcca gcgcccggtg catactttca
    11101 tgatgcagta cgacataaag ccgagtgcaa tcccttcggt gatcgagaaa gtaaagggca
    11161 tcatcaccgt ggtaataaat gcgggcactg attcggtaaa atcatcccag ttaacgcgcg
    11221 ccaggctcga agtcatcagc acgccaacaa agattaacgc tccggcggtt gcgtaaggag
    11281 gaaccatcgc caccagcggt gagaagaaca taactaacag gaacataacg ccgaccacga
    11341 cagccgttag ccccgtgcga ccaccgactg ccacaccaga agtactttca atataggcag
    11401 taacagacga ggtaccgata aacgcgcccg ccaccgaact gacgctatca acatacagcg
    11461 ccttattcat attggggaat ttaccgttac tatctattaa gccagcttta tcagttacac
    11521 caattaatgt tcctgatgaa tcaaataggt tgatcagcat aaaggagaaa atgataccgg
    11581 cgagttcaag tgttaacgcg ccgctcaaat ctacttcacc aatgacgccg ctaatatcag
    11641 gcggaatgga atagacgccg ctaaaatgaa catcaccgaa aaataatcca cagcaagacg
    11701 tcaccacaat agaaaccagc accgcggcat gaaaatgacg tgatgacaac acggttataa
    11761 taaaaaaccc taaaatacct aacaacacac cgtgagaact taaatcgcca atcatcacca
    11821 gggtgtcttt attggcgaca ataacgccag tattttttaa tcccattaag gcgataaata
    11881 atccaattcc actggtgata ccaatacgta aacttaatgg aatgttggag atcatccagt
    11941 accggatacg aaacagcgtg agcaaaaata gcccaattgc accccagaat atcgcgccca
    12001 tcccggtttg ccaggagatc cccatagccc ccacgaccac gaaggcaaag aaggcgttca
    12061 gccccattgc cggagccagc gccacgggta agttagcaaa tatccccatc gcaatactgc
    12121 cgataccggc aatcaaacag gtggtaacaa acaccacttt cgggtccatt tgtgccgcgc
    12181 cgaggatttg cgggttcaca aaaacgatgt acaccatggt taaaaaagtg gtcatccccg
    12241 caatcaattc tgtgcggacg gtggtcccat gctgaggtag tttaaataat cgcgaaagcg
    12301 tccctgattc attactcacg taatcggtat tgtcattatt catttttttg tccattggag
    12361 gagatttaat cccttgcttt tgtaaaacaa atgacaacaa cggttcagtg ataattatca
    12421 catttcaatt gcacattaat ggatattctt taataatctc gcgacgtttc tttatgataa
    12481 ataataatca aattgataaa atcaaaatga gaaaaatatg aataattcta ttaaccataa
    12541 atttcatcac attagccggg ctgaatacca ggaattgtta gccgtttccc gtggcgacgc
    12601 tgttgccgat tatattattg ataatgtctc tattctcgac ctgatcaatg gcggagaaat
    12661 ttccggccca attgtgatta aaggacgtta cattgccggt gttggcgcag aatacactga
    12721 tgctccggct ttgcagcgga ttgatgcccg cggcgcaacg gcggtgccag ggtttattga
    12781 tgctcacctg catattgaat ccagcatgat gacgccggtc acctttgaaa ccgctactct
    12841 gccgcgcggc ctgacgaccg ttatttgcga ccctcatgaa atcgtcaacg tgatgggcga
    12901 agccggattc gcctggtttg cccgctgtgc cgaacaggca aggcaaaacc agtacttaca
    12961 ggtcagctct tgcgtgcccg ctctggaagg ctgcgatgtt aacggtgcca gttttaccct
    13021 tgaacagatg ctcgcctggc gggaccatcc gcaggttacc ggccttgcag aaatgatgga
    13081 ctaccctggc gtaattagcg ggcagaatgc gctgctcgat aaactggatg catttcgcca
    13141 cctgacgctg gacggtcact gcccgggttt gggtggtaaa gaacttaacg cctatattgc
    13201 tgcgggtatt gaaaactgcc acgaaagtta tcagctggaa gaaggacggc ggaaattaca
    13261 actcggcatg tcgttgatga tccgcgaagg gtccgctgcc cgcaatctca acgcactggc
    13321 accgttgatc aacgaattta acagcccgca atgcatgctc tgtaccgatg accgtaaccc
    13381 gtgggaaatc gctcatgaag ggcatatcga tgccttaatt cgccgcctga tcgaacaaca
    13441 caatgtgccg ctgcatgtgg catatcgcgt cgccagctgg tcgacggcgc gccactttgg
    13501 tctgaatcac ctcggcttac tggcacccgg aaagcaggcc gatatcgtcc tgttgagcga
    13561 tgcgcgtaag gtcacggtgc agcaggtact ggtgaaaggc gaaccgattg acgcgcaaac
    13621 cttacaggcg gaagagtcgg cgagactggc acaatccgct ccgccgtatg gcaataccat
    13681 tgcccgccag ccagtttccg ccagcgactt tgccctgcaa ttcacgcccg gaaaacgcta
    13741 tcgggtcatt gacgtcatcc ataacgaatt gattacgcac tcccgctcca gcgtctacag
    13801 cgaaaatggt tttgatcgcg atgatgtgtg ctttattgcc gtacttgagc gttacgggca
    13861 acgtctggct ccggcctgtg gtttgctcgg cggctttggc ctgaatgaag gcgcgctggc
    13921 tgcgacggtc agccatgaca gccataatat tgtggtgatc ggtcgcagtg ccgaagagat
    13981 ggcgctggcg gtcaatcagg tgattcagga tggcggcggg ttgtgcgtgg tacgtaacgg
    14041 ccaggtacaa agtcatctac cgttgcccat tgccgggctg atgagcaccg acacggcgca
    14101 gtcgctggcc gagcaaattg acgccttgaa agccgccgcc cgtgaatgcg gtccgttacc
    14161 cgatgagccg tttattcaga tggcgtttct ttctctgcca gtgatccccg cgctaaaact
    14221 aaccagtcag gggctatttg atggcgagaa gtttgccttc accacgctgg aagtcacgga
    14281 ataataaaaa aaaagcccgg cgtcatgccg ggcaaaagtc accagttacg tttatgccac
    14341 tgtcaactgc tgaatttttt tctcgcggcg gattttgcgt tcttccatca ccgccactat
    14401 cgccatcagg cagatacaac caatcgcggc gatatcaagt gcggcgaagg tgcctgccca
    14461 gccggtaagg ccgaataccg gcgtcccatc ggcaatcatt cccagaccta acttggcaaa
    14521 gctgtcacca atcaggtaag caaaggtgcc tttaatacca tcggcagcgc caatcgcttt
    14581 tttaggtaca aagccaacag cagccacacc aatcaacaat tgcgggccaa agaccaggaa
    14641 acccaacgca aagagagaag ccagatagat atattcgttg ctggcatgtt gatacacacc
    14701 gagcgtggcg ataatcagcg ccagcgcgat gcaggccacc aggccacggc gaccgttcgc
    14761 caggtcagag agccagcccc acagcagcgt accgaccagc gcaccagctt caaacagcgt
    14821 aaagccctga atcgccaccg ctttagagag tttcagttcc tggaacgcgt atacggttga
    14881 ccactggtcg ataccaatac gtaccacata gaggaaaatg ttggcgaagc acagcagcca
    14941 gatcactttg tttttcagca catactcaac aaagatctgc cacttggtca tatcggtaga
    15001 ttctgtctct ttgtcctctt cgctgatctc ctcgccgaac agttcttcag ctttgccgag
    15061 gccataagat tccggggagt cgctgccgta acgcaggccg ataaaaccga caatcagcgc
    15121 gataatcgac gggaagataa acatgccgat gacatggcca tcgaacaggt aatttgcgcc
    15181 gaacagcgcc acacctgctg cgcctgcacc gccaaggttg tgagatatgt tccagaaacc
    15241 gaggaaggtc ccgcgcttac gacgcggcgt ccatttggtg atggtggagt aactgcacga
    15301 accgccggtg ctctggaaaa agccgcttaa ggcgtagaag gcaatcatca gaaacaggct
    15361 aaccgagccg ctgcccatac tggcactgaa gcccagcata caaatggcag acaggataag
    15421 catgaacggc aggaattgtt tggtgttttt gccgtcggcg tagtaggaaa ccagcgtttt
    15481 acccacgcca taagtgatgg agaaacccag gccgatcatc cccaactgcg tcatgctcaa
    15541 cccgtaggtc gaaatcatat cgttctgcgc gatgttaaag ttcttgcgaa tcaggtacat
    15601 cgtcaggtag ccgataaaga ccaccaggta ggattgcatg aacggtttga accacatttt
    15661 gcgccgcact tcgagcggaa ggtccagggt cggcttgcga acctggttta agaaagccag
    15721 catgggttac tcctgaaatg aatacctgcg tgaggcatgc attgtaaaaa tcagcagcaa
    15781 atcaggcctg agatagcgtc caggcaaaac ctggaaaatt tcttagtttt gccggatatg
    15841 aagtgaaaag gtgagatgca tcacgcttcg cgcggtgcct gggcgttcaa aaagggcagt
    15901 aacagcagtg cggaaatccc ggcggcgata gagataacca caaaaaatcc gctccagtgc
    15961 caggtatcga gtactttcgc cagtggccaa ccagcaagcg acgcccccag ataagcaaac
    16021 aagccgacaa accccgtcgc cgcccctgcc gcctctttgt gggaacactc tgccgccgcc
    16081 atgccgatta acatctgcgg gccaaagaca aaaaaaccaa tagtgaagaa gcaggttgcc
    16141 tgcatcacgt agctggcaaa tggcatcagc cacagcgagc cgactgaaag caaaattccg
    16201 gcggcgaaaa tcaaattcat cggcccacgg ttgccgttaa acaatttgtc cgagccccaa
    16261 ccggctacca gcgcaccgat aaatccgccc agttcaaaca tcgtcactgc cgtattcgcc
    16321 gtgaccagat cgacgcccag cgtctcggac atatacaaat tgccccagtc gttgattgcc
    16381 gcccggacca catagaccag cacatagcaa aacgaaagca gccagatata cggattcagc
    16441 aacacatact tggtgaggat ctctttacgc gtcagccctg ccccttcttg ttgttgagca
    16501 atctccagcg cgtcgtgccg ccattcacct accgccggta aacctaacgc ctgcgggcga
    16561 tcgcgcagcc gccagcagag aaaaatcccc acgacgatcg ccatacaacc agcaatcatc
    16621 atcccggcac gccagccgta atgtagtgca gccgctgcca tcacaatggg aatgagtgcg
    16681 ccgccgacgt tatgcgccgt gttccataat gcccaccaac cgccgcgctc ggtacgagaa
    16741 taccaggcgg ttaacagacg cgcacacacc ggtgaacccc agccctggaa aaaggcgttc
    16801 agcacccaga gtacggcaaa cgcccatagc gacgtcgaga agccaaacag aatgttgata
    16861 atgcccgtgg cgataagccc tatccccata aaataacggg catttgagcg atcgctgaca
    16921 atgccggaga caaacttcga cacgccgtaa gtaatgtaaa acagggtcgc taacaggccg
    16981 atatcgctac ggctgagcac gccgttagca aggatttctg gtacggcggc gttaaaactt
    17041 ttccgcgtga agtaaaacag cgcgtaaccc agccagatgg tcagcagaat atgccgacgc
    17101 cagtagcgat agcgtgcatc aatttcatgt ttgtcagtca ttaatggcgc atcggcaggc
    17161 gctttcagaa acggcaacat catcgaacct tagacataac gttgaggtag agaaacgctg
    17221 acacgcgtgc cgtgcagaca ggaaatgtgt aatgtgccgc ccagcgccgt tacgcgctcg
    17281 cgcattccgg tgaggccaaa accttgttgc ccggaacccg gcggcaaacc gctgccatcg
    17341 tcttcaatca ccagcatcaa ccgttcatcc tgctgccagc cttgcagcgt aaccgcgctg
    17401 gcatcggcat gtttcacaat gttgttcagc ccttcctggc agacgcgaaa cagcgtcacg
    17461 cgctggtttt cacttaacgc tgactcatcg atacgccatt cgagatggct gacaatgccg
    17521 cgcccttcca gctccatttc ccgcatcagt gagcggatgg cctgctccag ggtgagatca
    17581 tccagctggc gcggacgtaa ccgccctaac aaacggcgca ccgcatcgta aacacccagc
    17641 gacagttgtt cgatgagctg cccgctctgc ttcacgctgg cgttatctgc cgccagtcgc
    17701 tggacaatgc ccgcctgagt acgaatggca gtgatggtct gaccgatatc gtcgtgcagc
    17761 tcacgcgcca catcacggcg cacgctctct tcggtttcta acaaacgttc agccagatgc
    17821 tgattgcgcg ccagttcctt ttgtagcgac tggttaagtt cacgcaaccg ctggatgcct
    17881 gcgcctagta acaaccctgt cagactttgc accagcaacg agagcaataa atccaccgga
    17941 tgatcgcgcc aggtttgact ggcgatcagc gcgatggcgt tcatcaacgt cgcaatcagc
    18001 gctccttgcc aaccatagtg ccaggccagc gcaataatcg gcagcgccag gcagaatggc
    18061 gtaaagcgcg acagttcgtc cggcaatccc aactggagcc agagactaat aacaaacagc
    18121 agcaagtacc agaccagatg ccgcccgcgc cagttgatcg gttgagaaac taacgacggg
    18181 ccgagcggca gccaggtgtt attggcgaga tagtgccaga acaccagaca tatcggggcc
    18241 agcgtcaggc cgccagttaa agtcagtagt agcgcattcc acgactcttt gccgtgccaa
    18301 agccagggca gcgactgcaa caacgccgcc gccgttaacg ccgccccctg taacagcaag
    18361 gtgcgccagt cacgctgatg gcgatagcgc gagatcagcg ctacgggcag taacgtcagt
    18421 aaactaccga tcatcaataa ggaaaaatgg gttaacccga ccgcctgcgt tagccagtaa
    18481 atcagcagcc attccgcgcc cagcaatacg ggccagtatc cgcgcgggca ttgcagcatt
    18541 agccccagac gcagaccaaa cggaaataac agcaccgcca tatcagggcg ctcaaccagg
    18601 tgcaggctga tactccacag gcaaaaccat gcggcagaga agataaaaaa gcaggcaata
    18661 acggtaatta agcgggagaa caacgtcttc atcaccagcc atcaaacatg cggcgcgcca
    18721 gttcaacgtc gttactgacg cccagttttt ccatcagatt ggcgcgatgg acgtgtaccg
    18781 ttttcggtga caagcccagt tcggcggcaa tctctttcac cgccattcct tgcgccagtt
    18841 tttccgccac ctggcgttcg cgtttggtca gcgggtcctg gcgaccggat gccagtttaa
    18901 tggcaatatc cggcgtcaga taacagccgc ccgtggcaac cgtatgcacc gccgcaatca
    18961 gttcgtcagg gctacagcgt ttggagagaa agccgcgcgc ccccgcgtta agcgcctgct
    19021 caaccagcgc cggactgtca tgaacggaga gcataattgt cgccatacct ttcggcagct
    19081 ggcttagcag ttccagaccg gagatatcgg gcatggagat atcgcaaata cacacctgca
    19141 c
//
LOCUS       DAZFKF010000098        19139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFKF010000098 DAZFKF010000000
VERSION     DAZFKF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.098"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(55..420)
                     /gene="yhaH"
                     /locus_tag="ACHKA1_004006"
     CDS             complement(55..420)
                     /gene="yhaH"
                     /locus_tag="ACHKA1_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5681583.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(713..1699)
                     /gene="yqjG"
                     /locus_tag="ACHKA1_004007"
     CDS             complement(713..1699)
                     /gene="yqjG"
                     /locus_tag="ACHKA1_004007"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO5681584.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(1769..2161)
                     /gene="yqjF"
                     /locus_tag="ACHKA1_004008"
     CDS             complement(1769..2161)
                     /gene="yqjF"
                     /locus_tag="ACHKA1_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO5681585.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2347..2646)
                     /gene="yqjK"
                     /locus_tag="ACHKA1_004009"
     CDS             complement(2347..2646)
                     /gene="yqjK"
                     /locus_tag="ACHKA1_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO5681586.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2636..3040)
                     /gene="yqjE"
                     /locus_tag="ACHKA1_004010"
     CDS             complement(2636..3040)
                     /gene="yqjE"
                     /locus_tag="ACHKA1_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO5681587.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3043..3348)
                     /gene="yqjD"
                     /locus_tag="ACHKA1_004011"
     CDS             complement(3043..3348)
                     /gene="yqjD"
                     /locus_tag="ACHKA1_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO5681588.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3386..3754)
                     /gene="yqjC"
                     /locus_tag="ACHKA1_004012"
     CDS             complement(3386..3754)
                     /gene="yqjC"
                     /locus_tag="ACHKA1_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO5681589.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3901..4284)
                     /gene="mzrA"
                     /locus_tag="ACHKA1_004013"
     CDS             complement(3901..4284)
                     /gene="mzrA"
                     /locus_tag="ACHKA1_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO5681590.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4288..4950)
                     /gene="yqjA"
                     /locus_tag="ACHKA1_004014"
     CDS             complement(4288..4950)
                     /gene="yqjA"
                     /locus_tag="ACHKA1_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO5681591.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(4972..5055)
                     /gene="yqiM"
                     /locus_tag="ACHKA1_004015"
     CDS             complement(4972..5055)
                     /gene="yqiM"
                     /locus_tag="ACHKA1_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO5681592.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(5295..6071)
                     /gene="exuR"
                     /locus_tag="ACHKA1_004016"
     CDS             complement(5295..6071)
                     /gene="exuR"
                     /locus_tag="ACHKA1_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO5681593.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQTADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6201..7499)
                     /gene="exuT"
                     /locus_tag="ACHKA1_004017"
     CDS             complement(6201..7499)
                     /gene="exuT"
                     /locus_tag="ACHKA1_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO5681594.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7982..9394
                     /gene="uxaC"
                     /locus_tag="ACHKA1_004018"
     CDS             7982..9394
                     /gene="uxaC"
                     /locus_tag="ACHKA1_004018"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO5681595.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9409..10896
                     /gene="uxaA"
                     /locus_tag="ACHKA1_004019"
     CDS             9409..10896
                     /gene="uxaA"
                     /locus_tag="ACHKA1_004019"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO5681596.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQKRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10979..11530
                     /gene="ygjV"
                     /locus_tag="ACHKA1_004020"
     CDS             10979..11530
                     /gene="ygjV"
                     /locus_tag="ACHKA1_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO5681597.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPSAVDERG"
     gene            complement(11534..12778)
                     /gene="sstT"
                     /locus_tag="ACHKA1_004021"
     CDS             complement(11534..12778)
                     /gene="sstT"
                     /locus_tag="ACHKA1_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO5681598.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(13333..14298)
                     /gene="alx"
                     /locus_tag="ACHKA1_004022"
     CDS             complement(13333..14298)
                     /gene="alx"
                     /locus_tag="ACHKA1_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO5681599.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(14582..15568)
                     /gene="ygjR"
                     /locus_tag="ACHKA1_004023"
     CDS             complement(14582..15568)
                     /gene="ygjR"
                     /locus_tag="ACHKA1_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5681600.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKQ"
     gene            complement(15647..16339)
                     /gene="ygjQ"
                     /locus_tag="ACHKA1_004024"
     CDS             complement(15647..16339)
                     /gene="ygjQ"
                     /locus_tag="ACHKA1_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO5681601.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATLFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARISAVIDAKIFHRQPKYLGPSVII
                     GPFSEHGCPAQK"
     gene            complement(16416..16919)
                     /gene="ygjP"
                     /locus_tag="ACHKA1_004025"
     CDS             complement(16416..16919)
                     /gene="ygjP"
                     /locus_tag="ACHKA1_004025"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO5681602.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            17004..18140
                     /gene="rlmG"
                     /locus_tag="ACHKA1_004026"
     CDS             17004..18140
                     /gene="rlmG"
                     /locus_tag="ACHKA1_004026"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO5681603.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCVLAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPQTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAREMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18424..18738
                     /gene="higB"
                     /locus_tag="ACHKA1_004027"
     CDS             18424..18738
                     /gene="higB"
                     /locus_tag="ACHKA1_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFO5681604.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            18735..>19139
                     /gene="higA"
                     /locus_tag="ACHKA1_004028"
     CDS             18735..>19139
                     /gene="higA"
                     /locus_tag="ACHKA1_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO5681605.1"
                     /translation="MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPAL"
BASE COUNT         4894 a         4952 c         4848 g         4445 t
ORIGIN      
        1 atcaacatga tgataaaagc cggatgagtc agacatccgg cgagcagatt tgttttaagg
       61 ttccagtttt ggatcaggtc caaagcggtt ttcgcctggc gtacccgcct ggcagttaaa
      121 gacgatgata ataagccagc cgataaacgg gattaagaat agcagtgccc accacgccga
      181 gcggtcggtg tcgtgcaggc ggcggaactg aactgcccac cacggtaaaa acaccagaat
      241 accgtagatt gtcgtcagga tgccttcgcc accagcgcgt tgccagccta acattttatc
      301 cagcagcccc agcacgaacg taaagatgat gttgaccaga ataaacatcc agtactcttt
      361 gcgccgcgca cgaccccgga aaccgacata attttttagt actttcagat accagtccat
      421 ttttgcctca attttgctca atcacttgtt tttaaagcag caaattaaga atagacgtga
      481 atgaaatggc agtaaatatt cgttcaagga attaataaag tgctgtgaag gcgcgctaag
      541 actgatgaca agcgcaaaat tgccagatgc gctacgctta tctagcctgc gcggtcattg
      601 caacatattg aatataaaag ttttgtaggt ttgataaggc gttcatgccg catccgacat
      661 gaacaacatg cacttagcta attatcagag gcacctcgta aggtgccttt ttttaaccga
      721 agcgaacatc tcgtccatgc ggttcatcga gatcctgcca cggaccaatt gaaataatcc
      781 ccgtagggtt gatggtctta tgactgcgga agtaatgatt acggatatga tcgaaattga
      841 ctgtttcggc aattcccggc atctggtaga tatcacgcag gaagccatac agattaaggt
      901 aatcgctgat gcggtgctta tcacacttga agtgggtcac atacactgga tcaaaacgga
      961 ccagcgtggt ccacaggcga atatcggctt cggttagctg gttgccggtc aggtaacgat
     1021 gctgacctaa aatctgttcc agtcgcgcca gcgattcaaa cactttctcc accgcttcat
     1081 cgtaggcttg ctggctggtg gcaaaaccag ctttatacac gccgttgtta acggtgtcat
     1141 aaatccagcc gttaagttcg tcaattttcg tttgcagggc tggtgggtag taatcacccg
     1201 ctttcgcgcc cagcgcatca aacgcggtat taaacatgcg gatgatttct gctgattcgt
     1261 tgctgacgat ggtgtggttc tttttgtccc acagcacggg aacagtaact cgtccgctgt
     1321 agtgtggatc ggcgtggaga taaagctgat acaaaaattc atgttgatag agcgtgtctc
     1381 cggttgctcc cggaaaactg tcgtcaaagg tccagccgtt ttccagcatc agcgggttca
     1441 ctacggaaac ggaaataaac ggttccagtc ctttgagttt gcgcatgatc agcgtgcggt
     1501 gcgcccacgg gcaggcgagt gaaacataga gatgataacg atctttctct gcgataaaac
     1561 cgcctgtgcc agtggggcct ggagcgccat cggcagtgag ccagttacga aatgcggaag
     1621 ctgaacgttg aaatttaccg ccggtagatt tggtatcgta ccaggtgtca tgccagacgc
     1681 cgtcaatcag ttgacccatt tttctctcct ccaatagcaa aaacgaggag cttcctcctc
     1741 gttttttatc gttaagtata gcgcgtgctt accacttttt attcagcagg cggtcgatgc
     1801 tatacgcgcc cggaccggta attgccagca gcaggaatcc gccagaaatt gtcaggtttt
     1861 tcatgaacat cagcgagttg acgccttcag caaagttgct gtgaaataaa aatgccgtca
     1921 gcagcgtaaa gcccgcagta aacagggctg tggtgcgagt caggaaaccg aacaggattg
     1981 ccagaccacc accaaactca agcagaatca ccagcggcag cataaaaccc gggacgccca
     2041 tcgcttccat atattgttgg gtacccgcgt aaccagtaat ttttccccag cctgcggtaa
     2101 taaacagaat cggcattaaa atgcgcgcta ccagtacacc aacatcttct aattttttca
     2161 tcatactctc cactgcgcca ctcaggctgc tgattgtttt tttgtgcaag ggcgcggtat
     2221 tagcgtcgtt gctgtcgatg gagagaatca taaacgtggt gaatgatgat tgttagcaag
     2281 gaaaactgtc aaaaatcttc aaaaaatttg atgggtaagg ccggaatggc tccggccaga
     2341 gagaagttaa ccgcgaagct gttgctgctt gagggtcgtt ttaaccagac gccaggcgct
     2401 ccatacgcca aaaccgcgtc tggcccagcg gaccagcata ttaggatggc gaatcgtcca
     2461 gatcgccatc acgctactgc caaccagcgc ccaggagcgc agacttagca gcatattcca
     2521 gcgacgatcg taagcgcctg ttgcctccag ccattcacga cgactggcag aaagatccag
     2581 ccgttgttgc tggatctggc taagcagttg cgccttacgt cgttcacgtt cgactttact
     2641 gctcacggga ctcctcctcg agcagctgcc gatcgtttgc cagctcatgg cgtgtatggc
     2701 gcagcaaggt ggatttacgc gatttacgta acgtccagat accgccaatc agtgccagta
     2761 gcagcaacac cacggtggtg gcaatcatcg cattcaggcg atattgcgga tcaaccgccc
     2821 aaataattag caccatcagg ctcataagac caaatgcagc gaaaagcatc gtcaggccca
     2881 gcatcagtaa aagttgaaag agattagctt tttcctcttc cagctccacc accgccagcc
     2941 gcagacgtgt ttccaccatt tcaaccatga tggaaacaat tcgctgcccg atgcccagaa
     3001 cgcttttacc gggcccttgc gcgtgatgag tgtccgccat aattaacgac gcgacagcag
     3061 aacgccgagc actacaccga ttgcagcgcc aatgcccacg cccgtccatg gattttcacg
     3121 cacatactca tcggcacgcg ccgccgcgac acgggtttgt ttggcgatgg catcaccggt
     3181 ttcacccagg cgataacggc tctgtttcag tgcctgctcc gctttgctac gaatcttact
     3241 caactcttct ttcgacttct cgccagatga gctaagcacc tcttccagcg tatcggaaag
     3301 ggatttcaac tcagcacgca gatgttccgt agtgtgttct ttcgacatag ttttctccag
     3361 gtgagtaagt agtgactatt gtgagttagt agtcgcgcgc ttccagcttt ttcagctctt
     3421 cctgcgcttc tgccagtttc cgttcgcgtt tggcaatctt atcggcatcg cctttttgct
     3481 tcgcctcggc taaatcttgc tggcgttccg ccacctcatc tttctgcttt gcgattttct
     3541 tctgatgatc ggcacgcagc tggctatctg aacagttggc ccggacttca ctcagggctt
     3601 tattcagacc gtcaatacga ttctggtttt ggtgtttttc ggcatagctg atctccttaa
     3661 ggatattttg ctccttttcc tgacacaggg tagtggcata actaccggca ctaagagcaa
     3721 aaagagaaac agctaaagcg atgcggtatt tcattcttga accttccatt gtgattcgga
     3781 cctattacaa aggaacgatt gtccagacaa cgaagcggta gtgttaacca ccgcctcatt
     3841 aatcatagac agttacggtg aaatcaccaa agtgagtgat ctgtttcgga taacaggaag
     3901 ttatccgaag cgatgtgagt tatcccgtag ccgggtcagc cactgcatag cctgactgtt
     3961 attgtcctgc tgcgcaatga tgtagccttg gggcaatgtt ctgtcgagga ccgcttttgc
     4021 agcggcgctc tggtcgctgg agtcaaaggt aattaacaga gtgtcgtttt tgggggtgat
     4081 acttttgaaa ggaatgccat gagcgtcaag gtgatgccag attgaaaaac cgtctggcat
     4141 cgttgtgcct tgatgaacgg cacgaatcgc cagcgtagac tcttgctggc gaaccgcgga
     4201 ccaggccagc aacagcgtgc cgaccagaag taaaacagcg ccggaccagg ctagctggcg
     4261 aagcgacatg cgaggtattt gcatccctta cccccgattt ccatatttct ttttccataa
     4321 cacgaccaga gaacctgcca ggccaaacac cagcagcacc accgggagca gcatcaggca
     4381 tgacatcagc tggtcctcgt actttaaaaa taccggcgtt ttgccgagca tgtaacccag
     4441 agttgtcagg atcaataccc acagcagacc gctcatccag ttgaaaaact gaaagcgcgc
     4501 gttattcagc cctgataacc cggcaatcgt cggcagcagt gttctgacaa acgcaataaa
     4561 gcgaccaatt aacagcgccg ataaaccgtg tttatgaaaa agatggtgtg cgcgttgatg
     4621 ataatgcgcg ggtaaatgag atagccagtt ttgtacggtg cgggtattgc ccagccatcg
     4681 cccctgaata tagctgaccc agcagccgag gctggcggca acggtcagca gcagaatcgt
     4741 ttgcggatag cccatcgcgc ctttcgcaat caacacgccg accaatacca gtaaactgtc
     4801 gcccggtaaa aaggccgccg gaagcaagcc gttttcaagg aacaaaatta caaacaagac
     4861 aaaatacaac atgccaatca tcgatggatt ggccagggtt tcaaaatcct gcgcccacag
     4921 ggcctgcagc aattgggtca aaagttccat tcattattcc tggtgaatcg gttaacgcca
     4981 cgctggtttt aaaagatgca ctaccgcaat gttattgctg ttttggtatg ttctgcgggt
     5041 ttgatacatc aacacagcct gaactattcc ctgttaaatc gtgagataag aaagcattca
     5101 aaaacataaa ttcaaattga atctaattgt aacaaaggtc aacgttgttc gttatcaaaa
     5161 ttacatgtcc gtggagtctg attttgctga atgcagggga gggtggcgag aggatttaca
     5221 caggctgaca ctttatactt ttcgcttacc gacgagacaa acaggcgcat tgcagcgcct
     5281 gtcggacagg aatttcattt actgccgctg gtggctgtat cgagatgcac caccgggttt
     5341 tcggcgaata gatagcggtc ggcattgaac tcgaagtcgt cgctggtttc gttgaataac
     5401 atgatcttgg tgttttccag gtgctgccac attgccagct tagcagcatg aggatctttg
     5461 cgaatcagcg ccttgaggat ttgatcgtgg tcatcacacc agttatcgac ggtacgggaa
     5521 tcaatgtgtt cgtgcagttt tttccagtac gggttatggc tacgctgggt ccacattttt
     5581 tcaacgatag ccgccagggc ggagttctgc gtcgccaggg ctacctgaat atggaactgc
     5641 aaatcccact cggaatcacg gaagcattgt tcaccgcgtg cctgctcctg gatggccatc
     5701 aatttcatga tgtcctgttt cgttacctga gttgccgcga actcggcaat attactttcg
     5761 atgagctggc gagcctggag taactcaaac ggaccgtaat tggcgaattc catattattg
     5821 tccgccgtct gctgatggcg cggctggttg gaaaccacgt gaatacctga acctttacgc
     5881 acttcgacat agccttcaac ttccagcatg atgatggctt cacgaactac cgtacggctg
     5941 acgttctttt catcggcaat aaagcgctcg gcaggcagtt tatcacccac cagatagacg
     6001 ccctgttcga tgcgctcttt caggtcagcg gcaagttgtt gatacaaacg gcgtggttca
     6061 gtgatttcca tatgcgctcc aggcatgata cggcagacta tatttgttat accacttttg
     6121 cgagtttagc tctactcgca gtgatgaaaa agccgcctga gaaggcggct ttgaagggaa
     6181 actaaaactt acacgaagcg ttaatgttgc ggcgcgggat cgttatgtgt ttcctgcgcc
     6241 acctcaatcg ccggtttgtt ctgcaacacg gtccagataa ccagcgcacc cagcaggtca
     6301 aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag tgcaccgaca
     6361 accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc gtttgccgtt
     6421 gccacttcgt tacgaccaaa cacgtcagaa gagagcgtaa tcagcgcgcc agacagtgcc
     6481 tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt ggtgaacaga
     6541 ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat cttacgggaa
     6601 acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc accgaggata
     6661 caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc tttcaggtta
     6721 aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc ccaggccggt
     6781 tctgccagga aacgcggcag cgcgataccc cagaactgac ggttacgcag gatctgacca
     6841 acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt aataatatag
     6901 tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa aatcagccat
     6961 gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg ccagctgtgc
     7021 atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga agaacctacg
     7081 ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga gctggctttc
     7141 agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc aacagccagg
     7201 ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag cacagcaaac
     7261 attgcataac cgattttcgt acccagcaca tccagtacat aacccgctac cggttgcatg
     7321 accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt ggagatgttt
     7381 aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag gtaaccaagc
     7441 acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat tttacgcatg
     7501 taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg ccagaggctg
     7561 aagatgttag ttacgaccgg gtaaccgaaa aaaaaccggc acgccccgaa cgttgccatg
     7621 aaagtttcgt gctaattcgg cttccgtacc ggttaacccg attcatctaa caaatgagtg
     7681 aatcggcaat ttgcattccg tcgacttatg atttgcgacg gcagaaagat aacttgtcat
     7741 acaactttaa aaggtgagag ccatcacaaa tgtgcgaata tttgtaggga cattacctga
     7801 cgacagcgag gccagtactg gcgcggcctg cagcgaaatt taccactttg agagtaattt
     7861 ttttaactac gtttattgat ctaactcacg aaaatatctt cggactctgg aaattggtgt
     7921 gataactttg tcagcatcgc accataagca agctagctca ctcgttgaga ggaagacgaa
     7981 aatgactccg tttatgactg aagatttcct gttagatacc gaatttgccc gccgtctgta
     8041 tcacgactac gcaaaagacc agccgatttt cgattaccat tgccatttgc cgccgcagca
     8101 gattgcggaa gactatcgtt ttaaaaacct gtatgacatc tggttgaagg gcgatcacta
     8161 caaatggcgc gctatgcgta ccaacggtgt ggctgagcgt ctgtgtactg gtgatgcgtc
     8221 tgaccgtgaa aaatttgacg cctgggcggc gactgttccg cacactatcg gcaacccgtt
     8281 ataccactgg acgcacctcg aactgcgtcg tccgtttggt atcactggca aattgctttc
     8341 tccgtcaact gccgatgaaa tctggaacga atgtaacgaa ctgctggcgc aggataactt
     8401 ctccgcgcgc ggcatcatgc agcagatgaa cgtgaaaatg gtcggcacca ccgatgaccc
     8461 gatcgattct ctggagcatc acgcagagat cgccaaagac ggctctttca ccatcaaagt
     8521 gctgccgagc tggcgtccgg acaaagcctt taacatcgaa caggcgacct ttaacgacta
     8581 catggcgaag ctgggcgaag tttccgatac cgacattcgc cgctttgctg acctgcaaac
     8641 tgccttgacc aaacgtctgg atcacttcgc cgctcacggc tgtaaagtgt ctgaccacgc
     8701 gctggatgtg gtgatgtttg ctgaagcgaa cgaagcggaa ctggacagca ttctggcgcg
     8761 tcgtctggct ggcgaaaccc tgagcgagca cgaagtggca cagttcaaaa ctgcggtgct
     8821 ggtgttcctt ggtgccgaat atgcacgtcg cggctgggta cagcagtacc acattggcgc
     8881 gctgcgtaat aacaacctgc gtcagttcaa actgctgggg ccggatgtag gctttgactc
     8941 catcaacgac cgtccgatgg cggaagagct gtctaagctg ctgagcaagc agaacgaaga
     9001 aaacctgctg ccgaaaacca ttctctactg cctgaacccg cgcgataacg aagtgctggg
     9061 caccatgatc ggtaacttcc agggcgaagg tatgccgggc aaaatgcagt tcggttccgg
     9121 ctggtggttt aacgatcaga aagacggtat ggaacgtcag atgacccaac tggcgcagct
     9181 cggtttgctg agccgctttg tcggtatgct gactgacagc cgtagcttcc tgtcatacac
     9241 ccgtcacgaa tacttccgcc gcattctgtg ccagatgatt ggtcgctggg tggaagcggg
     9301 cgaagcaccg gcggacatca acctgctggg tgagatggtg aaaaatattt gctttaacaa
     9361 tgcgcgtgac tacttcgcca ttgaactgaa ctaaggtctg gggttgatat gcaatacatc
     9421 aagatccatg cgctggataa cgtcgcggtc gctttagcgg atttggctga aggcacagaa
     9481 gtcagtgtcg ataaccagac tgttacgctg cgccaggatg ttgctcgtgg acataaattt
     9541 gcgttaacgg atatcgcaaa aggggccaac gtcatcaaat atggcctgcc gattggttat
     9601 gcattggcgg atattgcggc gggggaacac gttcacgccc acaatacgcg cacgaatctg
     9661 agcgatctgg atcagtatcg ctatcaacct gattttcagg atctgcctgc gcaagcggca
     9721 gatcgtgaag tgcagatcta tcgtcgcgct aacggcgatg tcggggtgcg taatgagctg
     9781 tggatcctgc caaccgtggg ctgtgtcaac ggcatcgcgc ggcagatcca aaagcgtttc
     9841 ctgaaagaga ccaacaacgc cgaaggtacc gacggcgtgt tcctcttcag ccacacctac
     9901 ggctgctcac agctgggcga cgatcacatt aatacccgca ccatgctgca aaacatggtg
     9961 cgccacccga acgcaggcgc agtgctggtg attggtctgg gctgtgaaaa caaccaggtt
    10021 gccgcattcc gtgaaacgct gggcgatatc gatcctgaac gcgttcattt catgatctgc
    10081 caacagcagg atgatgagat cgaggccggg atcgagcatt tgcatcagtt gtataacgtg
    10141 atgcgcaacg ataaacgcga gccaggcaaa ctcagcgaac tgaagtttgg tctggagtgc
    10201 ggtggttctg acggtctttc tggtattact gctaacccga tgctggggcg tttctctgac
    10261 tacgtgattg ctaacggcgg tactaccgta ctgaccgaag tgccggagat gtttggcgca
    10321 gagcagttgc tgatggacca ttgccgcgac gaagcaacgt ttgaaaaact ggtcactatg
    10381 gtcaacgact tcaaacagta ctttattgcc catgaccagc cgatctatga aaacccatcg
    10441 ccggggaaca aagcgggcgg tatcaccacg ctggaagaca aatcacttgg ctgtacccag
    10501 aaagcgggtt ccagtgtcgt ggttgacgtg ctgcgttacg gcgagcgtct gaaaactccg
    10561 gggctgaact tgttaagtgc gccgggtaac gatgccgtag cgaccagcgc cctggcgggt
    10621 gcgggctgcc atatggtgct gttcagtact ggtcgtggca cgccgtatgg tggatttgtg
    10681 ccgacggtga aaatcgccac caacagtgaa ctggcggcga agaaaaaaca ctggatcgac
    10741 tttgacgcgg gtcagctgat ccacggtaaa gcgatgccgc agttgctgga agaatttatc
    10801 gacaccatcg ttgagtttgc caacggtaag caaacctgta acgagcgtaa cgacttccgc
    10861 gaactggcga tcttcaaaag cggcgtaacg ctataaagtc gaaatgagtt gttaataaaa
    10921 aacggcgttt cataatgtga agcgccgttt gcttttccat acctgctaag gatttatcat
    10981 gaccgcgtat tggctggccc agggcgtggg tgtcatcgcc tttctgattg gtatcacaac
    11041 atttttcaat cgtgacgaac gtcgcttcaa aaagcagctt tcggtctata gcgccgttat
    11101 tggcgtacat ttttttcttc tgggcaccta tcccgctggt gccagtgcca tccttaatgc
    11161 cattcgtaca ttgattacct tacgcacgcg cagcttatgg gtaatggcga tttttattgt
    11221 gctgactggc ggaattggcc tcgcgaagtt ccatcatcct gtcgaactat tgccggttat
    11281 cggcacgatt gtcagtacct gggcgctgtt ccgctgtaaa gggctgacca tgcgctgcgt
    11341 gatgtggttt tcaacgtgtt gctgggtgat tcacaacttc tgggcggggt cgataggcgg
    11401 cacgatgatt gagggtagtt ttctgcttat gaatggcctg aatatcattc gtttctggcg
    11461 gatgcagaaa aggggaattg atccgtttaa agtagagaaa accccttccg ccgtagacga
    11521 aaggggttaa acattaatta cgcagggcgc tatttgccag acgatcgtct tccgcctggc
    11581 aagctgccgc agtgaacagc acgtcagttg aagagttcag cgcggtttcg caagagtcct
    11641 gcaatacgcc gatgataaag ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac
    11701 cgaacatatt acaggccagc gggatcagca gcagagaacc acccgccacg ccggatgcgc
    11761 cacaggcaca cagagaagcc actacgctca acagcagcgc tgtgggcaga tcgaccggaa
    11821 tacccagcgt attaaccgca gccagcgtca acacggtaat ggtgattgct gcacccgcca
    11881 tattgatggt ggcacccagc ggaatagaaa cggaataggt atcgcgatcc agattcagct
    11941 tttcacacag cgccatattc accggaatgt tagctgcaga gctgcgggtg aagaaggcat
    12001 acacgccgct ttcgcgcagg cacagcagca ccagcgggaa cgggttacga cgaattttcc
    12061 accacaccag caatgggtta accaccagcg ccaccagtaa catacagcca accagcacga
    12121 ccagcagttg cgcgtagccc cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa
    12181 ccagcccaaa aataccgatc ggtgcgaagc gaatgaccag tttcaccata aaggtgacgg
    12241 cattcgacat atcgttgacc aggtttttgg tggtttcgtt accgtgacgc agtgcgaaac
    12301 cgaggccaat tgcccacacc aggatcccga tgtagttacc tttcagcagc gcgtcaatgg
    12361 ggttggaaac catgctcatt accagtccgc gcatcacttc gacaatgcct gacggcggcg
    12421 aaatatcacc cgcgctactg gacaagtgca gggtagaagg gaaggcaaag ctgaagacta
    12481 ctgcggccag agctgcagag aaggtgccca gcagatagag gaacaaaata ggacggatat
    12541 tggttttctg cccgtgctgg tggttagcaa tagatgccat taccagcatc aacaccagga
    12601 tgggggcaac agctttcagt gcgccgacga acaaagtacc taacagacca acagcttccg
    12661 ccgcgggttt tgagatccat gccagaagaa tccccagaac aaggccgacc aggatttgtt
    12721 ttaccaggct gccatgagcc agacgccgga atagccccgg tgaacgttgc gtagtcattt
    12781 ttcgatcctt tcattgtgtt gtcgcacatc cctggtgcgt tctgtcgacg catctttcag
    12841 gatgtaacaa agtgtttgca aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa
    12901 aatgctggat tttaaagaga ggtcatgttt tgcaacttaa tggttacatt tatttgacat
    12961 aaacagaggg aaggactggc tactaaggct gattagataa catatgggca aaactatttg
    13021 gaatatgaga acggttgcgc tcctttaatg tgctatgcca tataaagtca ctggcacagg
    13081 ggcgcgcagg gggcggccaa tcgccgccgc cccctgcacc cccaggctca ggcgaacaaa
    13141 atcgccgctg cgcggttccc tcagcttatc ccttgcggct accgggtcgg gcgcaaggga
    13201 acgcttcggc gattttgacg ccaccaacac ccgtgcggtc tttttgtaaa gagctataag
    13261 ccgattacat aattaatagc tcacctttgt ctggaatgag ttatctctag taatggctta
    13321 atatacagcg aatcattctc cccgctgctt atcatgccga taattcaccc aggcgttgat
    13381 aataaacgtc atcaccagga tgccaaacac cacgcccagc gagacggcga ttggaatatg
    13441 gtagaagtcg acaatcagca tcttgatacc gataaatacc agaatcaccg ccaggccata
    13501 tttgagcatc gagaaacgct ccgcgacgcc cgccagcagg aaatacatcg cacgcaggcc
    13561 gaggattgca aagaggtttg aggtcagcac aataaacggg tcagtggtca cggcgaagat
    13621 agccggaatg ctatccacgg cgaaaatcac gtcgctcaat tccaccagaa tcaataccag
    13681 catcagcggt gtggcgtaca acaagccatt cttgcgcaca aagaaatgct cgttgtcgat
    13741 ggtgtcggtc atgcgcaaat gaccgcgtag ccagcgcacc agcggcttgt cgccaatgcc
    13801 tgattcatct tcatgggcga gcgccatctt cacgccggta aacagcagga aggcaccgaa
    13861 gatatacagg atccagtcga actgcgaaat cagccagctg cctgtgaaga tcatgatggt
    13921 acgcagaaca atcgccccga gcacgccata caccagcacg cgacgttgta atgccgccgg
    13981 aacggagaaa tagctgaaca acatcagcca gacaaagacg ttatcgaccg ccagcgattt
    14041 ctcaatcaga taacccgtga gaaaggccag tgcctgtgga tcggcaacgg cgcgaccttc
    14101 ggtttgcacc agataccacc agaaagcggc gttaaacagt aacgacagcg tcacccagac
    14161 cagcgaccag gccgcagcct gtttcatggt catggcatgt gccccacgac gcccctgcaa
    14221 caacaggtcg atagccagca taatggcgac aacaacagcg aatccgcccc atagcaacgg
    14281 cgtgccgaca gtattcatag aagttcctta cacataaaaa aaacggccaa cgtcggaaga
    14341 cgtcagccgc tgctttttat gcatagacct cgccttccgg caaggtctca cttacaacaa
    14401 aaaaggaata cgtctgcgta ttcctttcgg gttgcccggt gaccggatgt gatttttcac
    14461 acatcgtaat gacgatcgac cggcaatgaa gttactcccc tttgcaggta acaaagtatg
    14521 acagaccatt cacccagtca atggtctgtt acatgccttt tacactgctt tacggtaaca
    14581 attattgttt tacgctatct gccggaaaaa tcacccctgt ctggcggcgg atctctgtca
    14641 gcaatttggc ggtgatgcga ctgaccgcca gtcccggatg atccaccaga tgctcatcca
    14701 ccagggtagc gaacagctct gcttcgtaga gcatggtatt aatatgctgc ggctgggtga
    14761 gatcctgcat ctggctgcca cgcggcacaa agcatacttt ctggcattca gacagttttt
    14821 caatcaccag cgatcccgct tcgccctgaa tttcgcttgc caggacagaa tcactgactt
    14881 ttgagtgctg caacgtaacg ctgaaatcac cgtaatccat taccaccacg ccgtgggcat
    14941 ctacgccgct tgccagcaaa ctggcggttg cctgcacgct tttcggctca ccaaataacg
    15001 ccaccgccga cgccaggcag taaaagccga tatccataat tgaaccgtta gagaatgccg
    15061 gattaaaggt gttgggattc tcaccgtcca ggtaacgttg ataccgcgag gaatactggc
    15121 aatagttgaa aaaaactttc cgcagtttgc cgactttcgg cagcgcctgg cgcaacaaat
    15181 gaaagttcgg caggctggcg gttttaaatg cctcaaacag caccacctga ttttcccgcg
    15241 cacaggcaat ggcggcatcc acttccgcca gattcgacgc cagtggtttc tcgcaaatca
    15301 catgaatttt atggctaagg aaaagttgtg tctgggaaaa gtgcagggaa ttcgggctgg
    15361 caatatacac cgcgtcaatg gcatcgcttt ctgccatcgc ttccagcgac gtaaacagat
    15421 gctcgacaga aaaatcattg gcgaagtgct gggcctgttc aaggctgcgg gaatatacgg
    15481 cggttaactt gtatttaccg ctctcatggg cggcctcgac gaactggcga gtgatccagt
    15541 tcgtaccaat cacagcgaaa cgtatcataa cgcgtggata ctccataaag ggattctgcc
    15601 gtcaatgtag cacgcctttt tcatcttcga cgtgcgcgga tacgcattat ttctgcgctg
    15661 gacaaccatg ctcactaaac ggaccaataa tgaccgacgg cccgagatat tttggctgac
    15721 gatggaagat tttcgcgtca atcaccgcgc tgattctggc gagtttctcc cgtaactgag
    15781 tataaaaacc gtgcttcata ttcaggtctg gtgcgttaaa cccgatagca tcgatgccgt
    15841 attgttttgc cagccagatg gcgcgttgat tatgaaattc ctgcgagata atggtgatat
    15901 ggttttcccc aaacaccttt ttggcacgca ctaccgaatc cagcgttgag aatccggcat
    15961 agtcgcagaa gatcactttc gccggtacgc ctttagcgat caaagcctgc tgcatacctg
    16021 atgcttcatc gtaattttta cgtccgttat cgccactgac taacagccat ttcacttttc
    16081 cggcgtggta caactctgct gcggtatcaa tacgtcgggt aaagtagcga ttacccggcc
    16141 tggcccccag caataagccc acgttacgcg ccggaacgac gttgacgtcg ctccaggtca
    16201 gctgtttgct ggcgttgacc attacgcgat cggcaataaa aagggtcgct cctgccacaa
    16261 gtaacagtaa gcaggcaatc tttagagtcc ggcgagaaaa acaaatacgg agaagaaggc
    16321 gggcaaatgc gcgcagcatg gtcgttatcc ttaacgaaag gggggaggtc gattttaggc
    16381 gctaaagaaa gaaaagataa cgcctgacat cattttcaga ttttgtcctg cccaagcgac
    16441 aactgcgtta gccacagccg ggtgtcgaac tcaagctggt ggtactgcgg ctccatatgg
    16501 cagcacaatt gatagaacgc tttgttatgc tctttctcct tcaggtgcgc caactcgtgc
    16561 accacgatca tgcgcaaaaa cggttccggc gcgttgcgaa acacggtagc gacgcggatc
    16621 tctgcttttg ctttcagctt gccgccctgc acacgagaaa cagcggtatg taacccgagc
    16681 gcgtttttta gcacatggat cttattgtca tacatcactt tattgatcgg cggggcatta
    16741 cgcagaaact gatttttcag atcctgagta tattgccaga gggctttatc ggtggcgtag
    16801 tcgtgtgttc ccggatagcg ttttgccagc acatcgccca gacgctgttc gttaatcagt
    16861 gttcgcacct gggaaagtag ctgctcggga taaccctgaa gataagtaag attgctcata
    16921 accgcccacg aaaaagagaa aaagggtata ctcacgcacc ctttgcaggg gcaacgtcga
    16981 aattttatca ttcaggaggg ccgatgagcc acttagacaa cggtttccgt tcactgacac
    17041 tacaacgttt cccggcgacg gatgacgtta acccgctaca ggcgtgggaa gcggcggatg
    17101 aatatttgct gcaacagttg gacgacacag aaatccgcgg cccggtgtta atcctgaatg
    17161 atgcttttgg tgcgttaagc tgtgtgctgg cggaacataa gccgtacagc attggcgact
    17221 catacatcag cgaactggcg acgcgcgaga atttacgcct taacgggatt gatgaatcga
    17281 gcgtgaagtt tctcgacagc accgccgact acccgcaaca gccgggcgtg gtgctgatca
    17341 aagtaccgaa aacattggca ttgctggaac aacaactgcg tgcgctgcgc aaagtggtca
    17401 cgccacaaac acgtattatt gccggtgcca aagcccgtga catccacact tccacgctgg
    17461 aactattcga aaaagtgctc ggcccgacca ccaccacgct ggcatggaag aaagcgcgcc
    17521 tgattaactg cactttcaat gaaccaccgc tggccgatgc gccgcagacc gttagctgga
    17581 agctggaagg tactgactgg actatccaca accatgcgaa tgtcttctcc cgcaccgggc
    17641 tggatatcgg cgcgcgcttc tttatgcaac atctgccaga gaatctcgaa ggggagattg
    17701 tcgatctcgg ttgcggtaat ggcgttattg gtctgacgct gcttgataaa aacccgcagg
    17761 cgaaagtggt gtttgtcgat gaatcgccga tggcggttgc ttccagccgt ttgaacgttg
    17821 aaaccaacat gccagaggcg ttggatcgct gcgaatttat gatcaacaac gcgctctccg
    17881 gcgtggagcc tttccgcttt aatgctgtgc tctgcaaccc gccgtttcat cagcaacatg
    17941 cgctgaccga taacgtcgcc agggagatgt tccaccacgc ccgccgctgc ctgaaaatca
    18001 acggcgaact gtatatcgtt gccaaccgtc acctggatta cttccataaa ctgaagaaga
    18061 ttttcggcaa ctgcaccacc atcgccacga ataataaatt tgtggtgctg aaagcagtga
    18121 agctggggcg tcgtcggtaa gattgacagc tacctgccag ataaggcgta aacgccttat
    18181 ctggcatacg tttagcacat tacccccagg tcggataaga tgcggcaagc gtcgcatccg
    18241 gcaatgtctg cccagttctg gaatcgcgct ccataaaaca aaaaatccct gtcaccatcc
    18301 tctacattct cttgtttagc gttgttcaac gtttattctt ccgtcacaca gataaattca
    18361 tccgttgcca atctgtcaac ggatgttatc atattgccaa tttgtcaacg gacgtgatga
    18421 agaatgcacc tgataactca aaaagcattg aaagatgctg cggaaaaata cccgcaacat
    18481 aaaacggagt tggtggctct agggaacacg attgctaagg gatatttcaa aaaacctgag
    18541 tcattaaaag cagtattccc atctctggat aacttcaaat atctggataa gcattatgtt
    18601 ttcaatgttg ggggcaatga attacgtgtt gtagcaatgg tcttttttga atcgcaaaag
    18661 tgctacatac gtgaagttat gacgcataaa gaatacgatt tctttaccgc tgttcatcgt
    18721 actaagggga aaaaatgatt gcgattgccg acatcttgca tgcaggagaa aagctgactg
    18781 ctgtggcacc ttttctggcg ggtattcaga acgaggaaca atacatccag gcgctggaac
    18841 tggtagatca tctgctgctc aacgatcctg aaaacccctt actggatctg gtgtgtgcca
    18901 aaataaccgc gtgggaagaa tcagcgcccg aatttgcgga atttaatgcc atggctcaag
    18961 ccatgcctgg cggtatagcc gtgattcgta cccttatgga tcaatatggt ttaacccttt
    19021 ccgatctgcc ggaaattggc agtaaatcta tggtgtcacg cgttttgagc gggaagagga
    19081 aattaacgct ggaacacgct aaaaaattgg caacgcgatt cggcatttct cccgccttg
//
LOCUS       DAZFKF010000099        18687 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFKF010000099 DAZFKF010000000
VERSION     DAZFKF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.099"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..573
                     /locus_tag="ACHKA1_004029"
     CDS             <1..573
                     /locus_tag="ACHKA1_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO5681606.1"
                     /translation="GINAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNMFVAKTGSVG
                     AQADYSGPAWYLLDCSRAIKPLIYQDRRKAELVAQTKVDEGRAFTDNEFVFGASARRN
                     VGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVPTSLEKQAVQ
                     LLERELFADGNATVSNEMKGKLELVVADYL"
     gene            617..1000
                     /locus_tag="ACHKA1_004030"
     CDS             617..1000
                     /locus_tag="ACHKA1_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO5681607.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEAEPCLTVRILQTPADDAGSVAGVVHAVAGTDLAEAEAEAEA
                     EAEAGQDAPRKKTGNKAKQAKKAHA"
     gene            997..1416
                     /locus_tag="ACHKA1_004031"
     CDS             997..1416
                     /locus_tag="ACHKA1_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFO5681608.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            1413..1973
                     /locus_tag="ACHKA1_004032"
     CDS             1413..1973
                     /locus_tag="ACHKA1_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFO5681609.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQANSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            1974..2219
                     /locus_tag="ACHKA1_004033"
     CDS             1974..2219
                     /locus_tag="ACHKA1_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO5681610.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKTFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            2216..3718
                     /locus_tag="ACHKA1_004034"
     CDS             2216..3718
                     /locus_tag="ACHKA1_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO5681611.1"
                     /translation="MINFDGIGNDRRIPLIGVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGTGVLDVPVRITRASQAVELWGRGSMIALMVAEFIAINPDAELYAIAQGAGT
                     GGANAAAFNLTGTAATDGLLCIYIGGRRYALPVKAGQKGKAIIDALVALINKDTDAPF
                     TAVSAVVDGSNAGGLKGSMGVSARFIGECSVHDLRLNYHDGETLPSGLTPQTGFPSSR
                     ARNPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWLAHTG
                     TFGEVQAFGAKRNDFLATCTSIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALP
                     SRMAPAPADRLTREERNSLLWGGIATVSVAAGDMVQIERQVTMYRQNVYGESDPSYLD
                     VETIYTLSYLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWV
                     ELGLVENLDTFKKNLLVERNTSDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            3727..4092
                     /locus_tag="ACHKA1_004035"
     CDS             3727..4092
                     /locus_tag="ACHKA1_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO5681612.1"
                     /translation="MSGKQYQGTATIRVNGQEYDTLEGATFSPSGYEREVVKGAKVYG
                     YREKPREATLDCKFPAGGEGSPAADEINTWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGDISAKFASATSTRVQ"
     gene            4107..4583
                     /locus_tag="ACHKA1_004036"
     CDS             4107..4583
                     /locus_tag="ACHKA1_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO5681613.1"
                     /translation="MSTRKKKTAVSDEAVMEAIRDALEDGDPRTAGLAEQLVNGYVDL
                     LDGLPFGESREYRVTFRELTAKDSIDAESEAERLMETRNGPVLVASPALRGIALLRRQ
                     IAAVGQIEGPLSPRQIGQLSERDLSRLMAAVSLLDSAMAGKLVAERGRPDAVPGSD"
     gene            4711..6768
                     /locus_tag="ACHKA1_004037"
     CDS             4711..6768
                     /locus_tag="ACHKA1_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO5681614.1"
                     /translation="MAKNLKASLIVDLLGNISAKSRQWSQELGAFSRSGRTGLGGLGN
                     AARRAGQETELVGSRMQRTLAGVRGSIRTVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNKAFIRPAAEMENYILRLNAINHGDKAKTEAVKAWAVQNAKDTTWGLAGVMQ
                     EYASSRGFGMSDREARRFITMLQDQGGYHGWSLSDAQGASLQLKQMFARQSIQAADAN
                     ILTGYGINVYQLLADKLGVNQKLIREKGEKGKLGPDSIRLLFQVMAEQAKGAQKNAMN
                     SWTGMTSMMGDVWDQFAREVMAKGPFDSLKKSLKGFLDYADAAQKSGLQDKLATQTAS
                     ALNQGFEYARDAATGFYRAIQKVRETLQALRDAGYGDALDRIGQGAQTAAKYLMYMYL
                     ASRALKVLRFAGAGALRLGATPLRYSMAMTSVLTSPFRKPQTTVPGTQPGRAGRFLNF
                     LTGVNPAAVQPVLVTNWPAGGLASGGGDVVVSGDGKTVRGRKKRGPGRGRGVTTVVTA
                     GEQLAESAGKQGFFGRMMSRAGGLVTAAGNRMGLGRFAGLFRGAGRLGGGALWAGAMA
                     APVLLDSSASAADKAGAVGSLAGSIAGGALGAAAGPVGVAIGSTVGSYLGDYLGGWLT
                     QAWQKLRGSSDENGGQATAKTAARVELVAPEGWRARSIDVDDTAQHGLDVNVWNGGNY
                     GLY"
     gene            6755..8113
                     /locus_tag="ACHKA1_004038"
     CDS             6755..8113
                     /locus_tag="ACHKA1_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HFO5681615.1"
                     /translation="MASTDTPGRGSFRGVPFLVYQEQRERGGRNIVRREYPLRESGGA
                     DDLGPKLPEFTFTVLVTGDDLQTQRSRLRDALRAPGAGELMHPDYGTLNVLINSFESR
                     YNASEQGTVEFTINVIPAIDDTAPSVAEDTAAALEQKSGSAMNQLFNTLSDGWTVISD
                     GLHDVQAMTDTISDKIDALENAVSGMGIVQDVSAFTASFTALKGNAAALINAPRRMAE
                     SLAGIFAVLTGLPGNPSLSLTGKTGRPSGSLTTNRDSMTEQAMPQLYRTLSSLRYTLS
                     EQDDPQRLIGLTPAAQKNIRLLRSVMQSATLVSQAQTVGKLLDQVIRQNTTHVQDTDR
                     AGGLAWLESSADVQRINRDLSDAMEQQVLDLSAQGHTGTALALRDAGLALTEDLTTRS
                     VHLPGAFQVMVRTTEPALVTLYRATGNSRRWQYFVRRNNIPDPVFVPGGRSVEVISEQ
                     QD"
     gene            8097..9221
                     /locus_tag="ACHKA1_004039"
     CDS             8097..9221
                     /locus_tag="ACHKA1_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO5681616.1"
                     /translation="MSSRIELYIGGSIFSGWLTVSVRRSLEHLAGSFELGLMLPGERI
                     PSAMRTGQSLTLRINGQTVISGWLDQVSQRISATRHQISISGRDKTGDLVDCAAIHPG
                     SQWRNRTLAQIAADLCAPFGIAVRWQVNDDTAARPFSSFTLENSETVADALTRSARHR
                     GVLVTSNADGDLVFTQAGSQQTDRLVLGDNLLDADYNTDWRGRYSEYRVRGHGRGGGK
                     RGDSESAARLAAPVGVISDEQIGRYRPKIILADQQTDTTGARQRALREMRRAIARSER
                     FSATVRGWFRDDGRLWDVNLLTGVSAQRFGIEQTELLVCQVEFLLDEQNGEVTRLVLA
                     PRDGFIVPAEPDSKGRGGSGDDVDAFIRQQMKKQGISFDE"
     gene            9211..9825
                     /locus_tag="ACHKA1_004040"
     CDS             9211..9825
                     /locus_tag="ACHKA1_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO5681617.1"
                     /translation="MMNDEVFSRLIAPVTRGIRLLFGRGVLTGTHDELKMQNVQLTGM
                     DGETFDDVERPQQYGQISVPLPGAETFFACLGGQRDQTVVLVVEDRRSRPTGLKAGDT
                     GVYHHEGHRIRLTKDGRIIVTCKMLEIYADEGMRVDTPEATFTGNVTVDKNLHVKGNF
                     ALDGTGKSQGLFTMSDAVIAGIRYSGHVHQDNGKGSKTGAPENG"
     gene            9818..10255
                     /locus_tag="ACHKA1_004041"
     CDS             9818..10255
                     /locus_tag="ACHKA1_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO5681618.1"
                     /translation="MADIAIVWDQGCGSLQLNGADLLTDDSLLTAVLISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPVGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHDRLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            10255..11337
                     /locus_tag="ACHKA1_004042"
     CDS             10255..11337
                     /locus_tag="ACHKA1_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO5681619.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYAQAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVVFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            11328..11885
                     /locus_tag="ACHKA1_004043"
     CDS             11328..11885
                     /locus_tag="ACHKA1_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO5681620.1"
                     /translation="MAVTLTPHQRALLQLLPDGLVWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPESQWISIVTVKTAVGYRHMNVLDDILTPLRIYDASALE
                     CILNRYKPAWQTFLYLYENSHEETE"
     gene            11885..12796
                     /locus_tag="ACHKA1_004044"
     CDS             11885..12796
                     /locus_tag="ACHKA1_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5681621.1"
                     /translation="MYFVDNNSGVTDMPPLAPSQGTQVKWFTEGDGRKGISHIGQDWL
                     NIVQAELLAILTEGQVQPDKAKLNQLVIAIKAIIAANAYSRKNNLKEIADAGAEAQAA
                     ARRHLGLGGLSGKDSLAANDVGALEKSMNFGDVPDKTSARQNLDVYSKSEGDNRYLRK
                     DQNGADIADKGTFIDNVGLRETVNKAANALPSDGTAAAANKLATPRNINGVPFDGTQD
                     ISITSGMTQSTADDRYVQNVQLGAQSYHSPGGNEMSWNYSAPSGCMLSGINVQETGKN
                     SADNIGGVYFRPVQIYISNAWRTVSSV"
     gene            complement(12831..13352)
                     /locus_tag="ACHKA1_004045"
     CDS             complement(12831..13352)
                     /locus_tag="ACHKA1_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5681622.1"
                     /translation="MQLINVKRYYPENTLYGNDVQYFQSEDGKDFYESLPLFTKKYKL
                     CITPDSGIICSISQDASALYPAGFSVVEVDELPEGADISGNWKFDNGVISRIPVNYAR
                     KLEAMRQLYLNQAYEKINDWRTELQLGTISDEDRAALTRWMAYISQVKKMEPSAIKTE
                     AEFDAIKWPEQPE"
     gene            13708..13893
                     /locus_tag="ACHKA1_004046"
     CDS             13708..13893
                     /locus_tag="ACHKA1_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681623.1"
                     /translation="MLISCVPSKGTPLIFLGVASLFAAAAVPSDGRAFAALFTVSRKP
                     RFQGCRISALKGLSTSF"
     gene            13907..14467
                     /locus_tag="ACHKA1_004047"
     CDS             13907..14467
                     /locus_tag="ACHKA1_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5681624.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDSIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYFPASVSEK"
     gene            14558..16639
                     /locus_tag="ACHKA1_004048"
     CDS             14558..16639
                     /locus_tag="ACHKA1_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5681625.1"
                     /translation="MNIMTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMT
                     FADCLISFNTRDCLWVRPVSGDPNVEITGAGIGAVIPLSADVAGTTEPSDWNNAETYT
                     SSAGDKVAGQTPSWYYVVVIAGQSNASSFGEGLPLPDTYDRPDPRIKQLARRNTVTPG
                     GVECAYNDIIPADHCLHDVLDMSNHNHPKADLSKGQYGCVGQGLHIAKKLLPFIPEEA
                     GILLVPCARGGSAFTDGADGEFTEASGATSASSRWGVNKPLFSDLVNRTKAALSSNPR
                     NILLSVVWMQGENDLKTGKHAEHSGLFVTMVETFRRELSAFSAQSPAGDMASVPWICG
                     DTTYYWKNTYPEQYKAVYGKYANEAKNIHFVPFVVDENGKNVPTNNPDEDPDIPGIGY
                     YGAISRDSTNWTSNDRASHFSSWARRGIISDRLATAILTLSGQKSAFFRGVTATEHSD
                     TGKSEPEPTGDVKDVSDQMKIIVSYTANAPTEPMKDQGWNAASGKATFVTDAAATGGN
                     KALRVNKAPNSMASWKMYHPITADNARDLFARGGDISLRFKIPSDAGIAENRYGVGIY
                     WAVSNWPDGDGKNNLIASCFLQTDSANLNVMYHKGSGSQQIGSYGVFDHEWHTLSLRF
                     AGGNSMSVTPVLDGKDGEPFELVKWVNAGFTVDQLTLTDITGNAATYPMLFDTIKVQV
                     NDAVSSSNVNK"
     gene            16675..16854
                     /locus_tag="ACHKA1_004049"
     CDS             16675..16854
                     /locus_tag="ACHKA1_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5681626.1"
                     /translation="MPILHTFMLYFCTCVCLLYLLSGGYKIIRNYFRNKIDAAAEEKI
                     KSGAVMPQPKDSGQI"
     gene            16890..17135
                     /locus_tag="ACHKA1_004050"
     CDS             16890..17135
                     /locus_tag="ACHKA1_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5681627.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGHPPAENSTENEPQPVEQPEPVQPGTDSAMM"
     gene            17210..17410
                     /locus_tag="ACHKA1_004051"
     CDS             17210..17410
                     /locus_tag="ACHKA1_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681628.1"
                     /translation="MDKNKKQPDTKGLEILSAEIINALRSGLDENTLQDRNFTTAMWI
                     AMQILCTPRCAIFAVREGQLTR"
     gene            complement(17597..17707)
                     /locus_tag="ACHKA1_004052"
                     /pseudo
     CDS             complement(17597..17707)
                     /locus_tag="ACHKA1_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17730..17951)
                     /locus_tag="ACHKA1_004053"
                     /pseudo
     CDS             complement(17730..17951)
                     /locus_tag="ACHKA1_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18066..18266
                     /locus_tag="ACHKA1_004054"
     CDS             18066..18266
                     /locus_tag="ACHKA1_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5681629.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPYHQHA"
     gene            18220..>18687
                     /locus_tag="ACHKA1_004055"
     CDS             18220..>18687
                     /locus_tag="ACHKA1_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFO5681630.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTQNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNF"
BASE COUNT         4454 a         4635 c         5370 g         4228 t
ORIGIN      
        1 gggattaatg cggcctgcta tgacggccag aacttctttg ataccgaaca tcctgtttac
       61 cccaaagtgg atggctccgg ggatgcgcag atggtcagca atatgtttgt ggcaaaaacc
      121 ggctctgtcg gtgcacaggc ggattacagc ggtcctgcct ggtatctgct cgactgttca
      181 cgcgcaatca aaccgctgat ttatcaggat cgccgtaagg ctgaactggt tgcccagacc
      241 aaagtcgatg aaggtcgcgc attcaccgat aacgaatttg tgttcggtgc ttccgcccgt
      301 cgcaatgtgg gctacggctt ctggcagatg gcctacatga tgcagtcacc gctgacgctg
      361 gatgcactgt ggcacggctg gtcagccatg cgcgaattta ccgctgacgg tggccgcaaa
      421 ctcggcatca aacccaccca tattgttgtc cccacctcgc tggaaaaaca ggcggtgcag
      481 ctgctggagc gtgaactgtt cgcagacgga aacgcaaccg tctctaacga gatgaagggc
      541 aagctggagc tggttgtcgc ggattatctg taaacggtga gccgggttta cggcccggcc
      601 cctgcggagg gcaaatatga atgaacatca cactgtggca gcaacagatg attcaggtct
      661 tcaggtcagt ggtgacaatt ctgtcacggt gctggcagaa gtgcgttgca gtcgttcggc
      721 gtttcgtcgt gcggggttcc tgttcacgcg tggacgtcag caggttgagg tcaccccgga
      781 gcagcttgcc cgactggagg cggagccatg cctcactgtg cgaatactac agacgcctgc
      841 tgatgatgcg gggagcgtgg cgggtgtggt tcatgcagtg gccggtacag atttagccga
      901 agccgaagcc gaagccgaag ccgaagccga agccggacag gatgctcccc ggaaaaagac
      961 cggtaacaaa gcgaaacagg cgaaaaaggc ccacgcatga attacgccac tgaaaccgat
     1021 atgcgggcgc gttaccgcga ggatttgctc aggccgttac tggcagtgcc ccgttcggat
     1081 gagccggaca cgcgcaagct gaaccgggcg ctgacagatg cgtcagccct tatcgacagc
     1141 tatctgtccg cccgttacac gctgccgctg gaggttattc cggcagttct tgttcagcac
     1201 tgttgcgcga ttgcctttta ttacctgtgc gatcagagag cctccgatca ggcgcgtgac
     1261 cgttaccgcg aagcgctggc ctggctgaaa gatgtcatga acggcaatgt gccggtcggt
     1321 gtggatacga acggtgcggc ccctgaatcc ggggatttac cacaggttca gtctgatgcg
     1381 gcggtattcg ggcgcaacca gaagggcttc atatgattac ggaaaccgaa caggcataca
     1441 tcgcccgtat ccgtgagtat ttcgggaatg aacttgtgtc tgttgacacg catcccggcg
     1501 actggagcga cagcgtactg cgcaccatgc tgattaacgc tccggcgatc tacgttgcct
     1561 ggctgggtgc cggtgaaggc cgtacccgtg gtcgcctggt cagtcactgg gtgttctacg
     1621 tcatcggcga catgctcaac gggcgtgagg ccagccgtcc cggactgtat cagattgtgg
     1681 cccggctgat tgccgtgctt aacggcttca gaacagaaaa aacctcaccg ctttactttg
     1741 aaaaggcggt caacggttac accgaaaccc aggcgaacag tggggccgtg atgtatgcgc
     1801 tttatttctc ctgcgaggaa atgatcgcgc cactgacgga tatcagctcg ctggacgact
     1861 tcctgcgtca ttacgaaacc tttgtggagc cgcccggtac gccgccgttt gaggcacata
     1921 ttgatttgcc ggggaacacg acaacggcgg tcgaaccgcc ggaggaactg taaatgaaaa
     1981 ccatctttat caaacccgcg ccgggacgcc tgattcgtga tcccgacaca atgcgcccgc
     2041 tggcacagga gggggaggaa aaaaccttca cgccgttctg gtgtcggcgt cttgatgacg
     2101 gcgatgtcat tcaggctgaa aaagccgctg aagaggctcc ggctgtatct gcggatgcaa
     2161 cgactgcgac agaaaaaccg gtcgcccaac cggcatccga taaggagaaa ccgcaatgat
     2221 taattttgac gggatcggta atgaccgccg tataccgctg attggggttg agtttaataa
     2281 ctccatggcg gtcaccggca ccccggcaca gcgtcagtgt gtgctgctgt ttggtcaggc
     2341 gagaatgaaa gagaatgctg tggacggtac cggtgtgctg gatgttccgg tcaggatcac
     2401 tcgcgcctct caggctgtgg agctctgggg gcgtggctcc atgatcgctc tgatggtggc
     2461 tgaatttatt gccatcaatc ctgatgcgga actgtatgct attgcgcagg gggccggaac
     2521 aggcggggca aatgccgccg cctttaacct taccggtacg gctgcgaccg atggtttgct
     2581 ctgcatctat attggtggac gacgctatgc gctgcccgta aaagccggac aaaaaggaaa
     2641 agctatcatt gatgcgcttg ttgccctgat aaataaggat actgatgcgc cattcacagc
     2701 tgtatctgcg gttgttgatg gcagtaatgc aggcggtctg aaaggaagta tgggagtgag
     2761 cgcccgcttc attggtgaat gctctgtgca cgatctgcgc ctgaattatc acgacggtga
     2821 aaccctgccg tcagggctga cgccccagac aggctttccc tcctcaagag cccgcaatcc
     2881 cgatatcacc cgaagtgtgg ccggaatggg ggatcgccag tacaactatg ttgtcatgcc
     2941 ctacaaggac gatgcaaacc tgaaaatcat cagtgatgaa cttctgaaac gctggggacc
     3001 ggccaaaatg tctgacggcg tgctgtggct ggcacatacc ggcacgtttg gtgaggtgca
     3061 ggcattcggg gcaaaacgca atgattttct ggcgacctgc acatccattc ccaaagcccc
     3121 tgaaccggat tacctctggg cagcggcggt ctgtgccacc tgtgcaccct ctctgggagc
     3181 agatcctgcg cgtccgcttc agacgctggc gcttccgtca cgcatggccc cggccccggc
     3241 cgatcgtctg acgcgtgaag agcgtaacag tctgctgtgg ggagggattg ccacggtcag
     3301 tgtggcggcc ggggatatgg ttcagattga gcgtcaggtg acgatgtatc gccagaacgt
     3361 gtacggcgaa agtgatccca gctatctgga tgtggaaacc atctacaccc tgtcatatct
     3421 gcgttattcc ctgcgcactt tcatcactca acgttttccg cgccataaac tggctgatga
     3481 cgggacgccc gttcgtgccg gtcagaatat cgtgacgccg gagatcatga agcttcagtt
     3541 aatttcgctg ggtgaagagt gggtggaact cgggctggtc gaaaatctgg acacgtttaa
     3601 aaagaatctg cttgtggagc gtaatacgtc agatcgaaac cgccttgacg tgttatgcac
     3661 gccggatctg gttaaccagt tccgcttcct ggcagcacag atccgtttca ttttgtgagg
     3721 taaggcatga gcggaaaaca gtatcagggc acggccacca tccgtgtgaa cggacaggag
     3781 tacgacaccc ttgagggggc cacgttctcc ccgtccggct atgagcgtga agtggtgaaa
     3841 ggcgcgaaag tctatggcta tcgcgagaaa ccccgtgagg cgacgctgga ctgtaaattt
     3901 ccggctggcg gagaaggttc accggctgcc gatgaaatca acacctggac tgcggtcacg
     3961 attgagtttg tggccgatac cggtgaagtc cacatgatga cgaaggcctg gagcagtgaa
     4021 ccggcctcgc ttgacggtgg cggtgatatc tccgcgaagt tcgccagcgc caccagtacc
     4081 cgtgtccagt aatcaggaaa aatgtaatga gcacacgtaa gaaaaaaacg gcggtttctg
     4141 acgaggccgt gatggaggct atccgggatg cacttgaaga cggcgatccc cgcaccgccg
     4201 ggctggctga acagctggtg aacggctatg tggatctgct cgacggcctg ccgttcggtg
     4261 aatcccgtga ataccgcgtc accttccgcg aactgacggc gaaagacagc attgatgcgg
     4321 agagtgaggc cgagcgtctt atggaaaccc gaaacggtcc ggttctggtg gcctctccgg
     4381 cactgcgcgg cattgcgctg ctgcgccgtc agattgccgc tgtcggccaa attgaggggc
     4441 cattatctcc gcgtcagatc ggacagctca gcgagcgcga tctctcccgt ctgatggcgg
     4501 cggtaagcct gcttgattcc gcgatggcag gaaagctggt cgctgaacgg gggcgaccag
     4561 atgcagtgcc gggatcagat tgaagaagcc gcaattgtgc tggggatggt gacaaaaagc
     4621 ggcccggagt gggcgcttaa cctcccctta tcgcagcttt accggcactg ccgacagacc
     4681 gaaaaaattc tcagaacgaa gcagtaaccc atggcaaaaa acctgaaagc ctccctgatt
     4741 gtcgatttac tcggcaatat ttccgccaaa tcccgccagt ggtcacagga gctgggggcg
     4801 ttctcccgtt ccggcaggac aggcctgggt ggtcttggaa atgcagcccg ccgggccggt
     4861 caggaaacag aacttgtcgg ctcccgtatg cagcgaacgc ttgccggtgt tcgtggcagc
     4921 atccgcacgg taacgtcaga ttttgaccgc ctgcagggca gcattaccgg caccatcggg
     4981 cggataagca acctttacgg gatgctggcg ggaggggctg cggtctacgg ttttaataaa
     5041 gcctttatcc gcccggccgc agagatggag aactatattc tgcgtctcaa cgccattaat
     5101 catggcgata aggcaaaaac ggaagcagtg aaagcctggg cagtacagaa cgccaaagac
     5161 accacctggg ggctggcggg ggttatgcag gaatacgcct ccagtcgcgg gtttggcatg
     5221 agtgacaggg aagcccgtcg ctttattacc atgcttcagg atcagggcgg ctatcacggc
     5281 tggtcactgt cagatgcaca gggtgcgtcc ctgcaactca aacagatgtt tgcccgccag
     5341 agtatccagg cagcggacgc caatattctg accggatacg ggattaacgt ttaccagtta
     5401 ctggccgata agctgggcgt aaaccagaag ctcatccgtg agaaaggcga aaaaggaaaa
     5461 ctggggccgg acagcattcg cctgttattt caggtgatgg ccgaacaggc aaaaggtgcc
     5521 cagaaaaacg ccatgaattc ctggacggga atgacgtcca tgatgggcga cgtctgggat
     5581 cagtttgccc gtgaagtgat ggcaaaggga ccgtttgaca gtctgaaaaa gagcctgaaa
     5641 ggcttcctgg attatgctga tgcggcacag aaaagcggct tacaggataa gctggccaca
     5701 cagacggcct cggccctgaa tcagggattc gagtatgcca gagacgctgc aaccggcttt
     5761 taccgtgcca ttcagaaagt cagggaaacg cttcaggcac tgcgcgacgc cggttatggc
     5821 gatgcactgg atcgtattgg tcagggggcg cagaccgccg caaaatacct gatgtatatg
     5881 tatctcgcct cccgcgcgct gaaggtgctc aggtttgcgg gggcgggagc attacgtctc
     5941 ggcgcaaccc ccttacgcta tagtatggcg atgacctccg tgctgacgtc gcccttccgc
     6001 aaaccgcaga ccactgtgcc gggaacgcag cccggacgcg ctggccgctt cctgaatttc
     6061 cttaccgggg taaatcctgc tgccgttcag cccgtgctgg tcaccaactg gcctgcgggt
     6121 ggcctggcct caggcggcgg ggatgtggtg gtcagcggtg acggcaaaac cgtgcgcggc
     6181 cgtaaaaaac gcggccccgg acgcgggcgg ggtgtcacga ccgtcgtgac cgccggtgag
     6241 cagctggcgg agagcgccgg taaacagggc ttcttcggtc gtatgatgag ccgtgccggt
     6301 ggtctggtaa cggcggccgg aaaccgcatg gggctggggc gttttgccgg gctgttccgt
     6361 ggtgccggtc ggctgggtgg gggtgccctg tgggccggtg ccatggcggc tcctgtactg
     6421 ctggacagta gcgccagtgc agccgataaa gccggtgccg tgggttccct tgccggaagt
     6481 atcgccggtg gcgcgctggg ggcggctgcc ggtccggtcg gggttgccat tggttccaca
     6541 gtgggcagct atctcggtga ttatctgggg ggctggctga cgcaggcctg gcagaaactg
     6601 cgtggcagca gtgacgaaaa cggcggacag gccaccgcga aaaccgccgc acgggtagag
     6661 cttgtggccc ctgaaggctg gcgggcgcgc agtattgatg tggatgatac cgcacagcat
     6721 ggcctggatg tgaacgtctg gaacggaggg aactatggcc tctactgaca cgccggggcg
     6781 tggctccttt cgtggcgtac cgttcctggt ttatcaggag cagcgggaac ggggcggacg
     6841 taacattgtg cgccgtgaat acccactgcg tgaaagcggc ggtgcggatg atctggggcc
     6901 aaaactcccg gagttcacat tcacggttct ggtgaccgga gacgatcttc agacacagcg
     6961 aagccgcctg cgtgatgcgc tgcgtgcacc cggtgccggt gaactgatgc acccggatta
     7021 cggcacgtta aacgtgctga taaacagctt tgaaagccgt tataacgcca gcgagcaggg
     7081 aacggttgag ttcacgatca acgtgatccc cgccattgac gataccgccc cttcggtggc
     7141 agaagatacg gcagctgcgc tggagcagaa aagcggttca gcaatgaatc agctgtttaa
     7201 cacgctttca gacggctgga cggtgatttc agacggtctg catgatgtac aggccatgac
     7261 cgacaccatc agcgataaga ttgatgcgct ggagaatgcc gtttcaggga tggggattgt
     7321 gcaggatgtc agtgccttta cggccagttt taccgccctg aaaggcaatg ccgccgccct
     7381 gattaatgcg ccccgtcgca tggctgaatc tctggccgga atatttgctg tgctgaccgg
     7441 actgcccggc aatccttccc tgtcactgac cgggaagaca ggaaggccgt ccggcagcct
     7501 gaccacaaac cgtgacagca tgacagagca ggcaatgccg cagctgtacc gcacactttc
     7561 ttccctgcgt tacaccctca gcgaacagga tgatccgcag cgcctgattg gtctgacgcc
     7621 tgcggcacag aaaaatatcc gtctgctgcg gagcgtcatg cagagtgcaa ccctggtgtc
     7681 gcaggcgcag acggtcggga aactgctgga tcaggtcatc cggcagaata caacgcacgt
     7741 tcaggatact gaccgtgcgg gtggactggc ctggctggaa agttctgctg atgtgcagcg
     7801 tatcaaccgg gatttgagtg atgccatgga acaacaggta ctggatctgt ccgcacaggg
     7861 ccataccggc accgcccttg cattacgtga tgcagggctt gcactgacag aagatttaac
     7921 cacgcggagt gttcatcttc ccggtgcttt tcaggtcatg gtgcgaacca ccgaaccggc
     7981 actggtcacg ctgtaccgtg ccaccggcaa cagccgccgc tggcagtatt ttgtccgcag
     8041 aaacaacatc cccgatccgg tttttgttcc gggtggccgc agcgtggagg tgatcagtga
     8101 gcagcaggat tgaactgtat atcggtggca gtattttttc cggctggctg acggtcagcg
     8161 tccgtcgctc gctggagcat ctggccgggt cgtttgaact ggggctgatg ctgcccggtg
     8221 agcgcatccc ctcagccatg cggaccggtc agtccctgac gctcagaatt aacgggcaga
     8281 cggtcatcag tggctggctg gatcaggtca gtcagcggat cagtgcaacc cgtcatcaga
     8341 tcagcatcag cggccgggat aaaaccggcg atctggtgga ctgtgccgcc atccaccccg
     8401 gcagtcagtg gcgcaaccgg acgctggcac agattgccgc agatttatgt gcgcccttcg
     8461 ggatagctgt gcgctggcag gttaacgacg acacggctgc acgccctttc agctctttta
     8521 cgctggaaaa ctcagaaacc gtggcggatg cgctgacccg ttctgcccgg catcgcgggg
     8581 tgctggtgac cagtaatgct gacggcgatc tggtgttcac ccaggcagga agccagcaga
     8641 cggacagact ggtgctggga gataacctgc ttgatgccga ttacaacacg gactggcgag
     8701 gacgatacag tgaataccgt gtccggggac acgggcgcgg tggtggcaaa cggggagaca
     8761 gcgagtccgc cgcccggctg gcagcacctg tgggcgtcat cagtgatgag cagatcggcc
     8821 gctaccggcc gaaaatcatc ctcgccgatc aacagacaga caccaccggt gcacggcagc
     8881 gtgccctgcg tgaaatgcgc cgtgcgattg cccgttcaga acggttttct gccaccgtgc
     8941 gtggctggtt ccgggatgat ggccggttat gggatgtcaa tctgctgacc ggtgtttcgg
     9001 cccagcgttt cggtatagaa cagactgaac tgctggtctg tcaggtggag tttttactgg
     9061 atgaacagaa cggggaagtc acccggctgg tactggcacc gcgtgacggc tttatcgttc
     9121 cggcagagcc ggacagtaag ggccggggtg gttccggtga cgatgttgat gcctttattc
     9181 gccagcagat gaaaaaacag gggatcagct ttgatgaatg atgaagtgtt cagccgcctg
     9241 attgccccgg taacgcgcgg tattcgcctg ctgtttggcc ggggcgttct gaccggcaca
     9301 catgacgaac tgaaaatgca gaatgtgcag ctcaccggca tggacggcga aacctttgat
     9361 gatgtggagc gcccccagca gtacgggcag atcagcgttc ccctgccggg tgcagaaacc
     9421 ttttttgcct gtctgggcgg acagcgggat cagactgtgg tgcttgtggt ggaagaccgg
     9481 cgcagccgtc cgaccggact caaagccgga gatacggggg tgtatcacca tgaggggcac
     9541 cggatacggt taacaaagga tggtcgcatt attgtgacat gcaaaatgct ggagatttac
     9601 gccgacgagg ggatgcgggt ggatacgccg gaagccacct ttacgggcaa tgtgacggtg
     9661 gataaaaacc tgcatgttaa ggggaatttc gcgcttgatg gtacggggaa atctcaggga
     9721 ctgttcacaa tgtcggatgc cgttattgca gggatacggt attccggtca tgtgcatcag
     9781 gataacggca aaggcagtaa gacaggagca ccggagaatg gctgatattg caattgtatg
     9841 ggatcagggg tgcggctcgc tgcaactgaa cggtgccgac ctgctgacgg atgacagtct
     9901 gctgacggcc gttctgattt cgctgtttac ggacaggcgg gcgctggatt ctgatgaaat
     9961 ccctgacggc actcgtgaca ggcggggatg gtggggagac agtttccggg agcgccccgt
    10021 tggctcccgt ctctggctgt taagccgtga aaagacgctg tcctccgtgg tcagccgtgc
    10081 acaggcctat gctgatgaag cgctggcgtg gctgcataaa agcggtgctg ccacatccgt
    10141 ggtatgtcat gccatgcgtg tggggcatga tcgcctttcg ctttccgtga aaatcaccct
    10201 gccggacgga agcagacatc cgatgatttt ttatgctgat atgaaggggg aatgatgcct
    10261 tatcagccct taccactggc gcaactgatc acacagacac aacaggatat cagccagcgc
    10321 ctgcccggtt cgcagccggg cgtgaatgaa accaccctga atgccattgc gtatgcacag
    10381 gcggggctgt cagcacagga gcatgaacat ctggcctgga tctcccggca gataattccg
    10441 acagaagctg atgaagccga actcctgaaa cactgcgcat tctggggtgt catccgtaaa
    10501 ccggcttccc gcgctgacgg accggtacaa ctgatgctga ccacggatgc agggatcacg
    10561 gaaggcgtac tccttcagcg aagcgatggt gttgtgtacc gcatcactgg ctctgcgacc
    10621 ggaaaagccg gaacactgaa tgttaatgtg gaggcggaaa gtgcggggcg cgccggaaat
    10681 accccgaccg gaacccgcct gtcctttatc acaccacagg cgggcatcaa ccagacagcc
    10741 acggtcaccg gcacgggact caccggtggt gcggatgtgg aaacggtgcc ggagttgctg
    10801 tccaggctgg tattccgggt acagaacccg ccatcagggg gaacacagta tgattttgaa
    10861 cgctgggcac gggaagtacc tggcgtgacg cgggcatggt gtaagcctga atggcctgag
    10921 gctggtagtg ttggtgtgac ttttgttcag gataataacc ctgacatttt ccccggggaa
    10981 ggtgatgtga agcgggtggc ggattatatc cgcagtcatg atgatccggc gacgggccag
    11041 cccgttggtc agccacttgg gccgacaatc agcgtgttta agctgaccaa taagccggtg
    11101 gtgtttgaga taaggattgt acccaaaacg ccggaaaatc aggctgccgt aaaacaggca
    11161 ttaacggacc tgctttataa cgaatcgcgg ccgggtggac ttgtattgcc ttcatcattc
    11221 tggcgggctg ttgcaggggt gaaaggactg gaggattttg aagttcgcag cccgctgaag
    11281 tccgtgatgg ccggagatac agagttgctg accgtggggg aaatcacatg gctgtaaccc
    11341 tgaccccgca tcagcgcgcc ctgttgcagt tgctgcctga cgggctggta tgggataagc
    11401 ggccgtcatc cgttcttgcg gctttgtgcc tgggcctcag tcattccacg gagcgtgttt
    11461 cctggaccgg caaccagatg ctggcagaac gttttcctga ttcatcccgt ctgctgctgg
    11521 aagactggga gcgttatctg gggttaccgg aatgtgatat gaccggcgca accattcagg
    11581 agcgtcagcg ttatgccggg aataaatacc ggatgaaacc ctctcttaac cgtgaatttt
    11641 atatccggtt tgcggcagag tttggttatg aaatagatat ccagccatca ccggaatcac
    11701 aatggatcag tattgtgaca gtgaagacag cggttggata ccgtcacatg aatgtactgg
    11761 acgatattct tacgccactg cgaatttatg atgcaagtgc gctggagtgc attttaaacc
    11821 gttataagcc agcctggcag acgtttttat atctgtacga aaacagtcat gaggagacgg
    11881 agtgatgtat tttgtcgaca acaattcagg tgtgaccgat atgccaccac tggccccctc
    11941 acagggcacg caggttaaat ggtttactga aggtgacgga cgcaaaggga tcagccatat
    12001 aggtcaggac tggctgaaca tcgtgcaggc agagctgctt gccattctga cagaaggaca
    12061 ggttcagccg gataaggcaa aactgaacca gctggtgata gccattaagg caatcattgc
    12121 agcaaacgca tacagccgga aaaacaacct taaggagatt gcggatgcag gtgcagaagc
    12181 acaggctgct gcccgccgtc atcttgggct tggaggatta tcaggaaaag acagcctggc
    12241 tgctaatgat gtgggagcac tggagaaatc catgaatttt ggcgatgtac cggataaaac
    12301 ctcggcccgc cagaatctgg atgtttacag caagagcgaa ggtgataacc gttatctgcg
    12361 caaggatcag aatggcgcgg atatcgcgga caaaggaacc tttatcgata acgtcggttt
    12421 acgggaaacg gtaaataagg cggcgaatgc cctgccatcg gacggcaccg ctgcggcagc
    12481 gaataaactc gccaccccaa gaaatattaa tggtgttcct ttcgacggga cgcaggatat
    12541 cagcattact tcagggatga cgcagtcaac cgcagatgac cggtatgtac agaacgttca
    12601 gcttggagca cagagctatc attcacccgg aggcaatgaa atgtcatgga attacagtgc
    12661 gccttccggt tgtatgcttt ccggtattaa cgtgcaggaa acaggaaaaa actctgccga
    12721 taacattggc ggggtttatt tccgtcctgt gcagatatat attagtaatg catggcgtac
    12781 agtttcatct gtctgatgaa agaaaaaggg tgcgataagc accctttaag ttattcaggc
    12841 tgttcgggcc atttaatggc atcaaattca gcttcagttt taatggcgga tggttccatt
    12901 tttttcacct gactgatgta tgccatccat cgggtaagtg cggctctgtc ttcatcactg
    12961 atggtgccca gctgtaattc tgttctccag tcattaattt tttcatatgc ctgattaaga
    13021 tataactggc gcattgcttc cagttttctt gcgtagttaa ccggaatacg ggagatcacg
    13081 ccattatcaa acttccagtt acccgaaata tcagcacctt cagggagttc gtcgacttca
    13141 accacagaga accccgcagg atacagcgcc gaagcgtcct gtgatattga gcagataatg
    13201 ccgctgtcag gtgttataca aagtttatat tttttggtga ataatggcag ggactcgtag
    13261 aaatccttac catcttcact ctgaaaatac tgtacatcat taccatagag ggtgttttcc
    13321 gggtaatatc gtttaacatt aatcagttgc ataaacatca ccatgtgaat caatgataaa
    13381 aacaggaata atactatcgc tatatacgga cagggattgt ctgagataac acatatatca
    13441 ggcgctgcct gccgtacgcc atgagccgtt aattaaatac ttgcagcgga tacccaattc
    13501 ccattaatac aatattgtat ctgtctgtaa taaacacggt catcaccacc acgcatttcc
    13561 cagtctccgt cagtcttaaa accggtaatc acatttcctg atgacaggga gtaattatat
    13621 tccagtacgc cgcttaatgc ctgcgctccc agtcttaccc ctgtaacata acgctgattg
    13681 gctgttgatt ccgtcatccc tgaagtaatg ctgatatcct gcgtcccgtc gaaaggaaca
    13741 ccattaatat ttcttggggt ggcgagttta ttcgctgccg cagcggtgcc gtccgatggc
    13801 agggcattcg ccgccttatt taccgtttcc cgtaaaccga ggtttcaggg atgccgtata
    13861 tctgctttaa aaggcctttc cacctctttt taaggaaggt aaatacatgc tgattggcta
    13921 tatacgcgta tcaacaaatg accagaacac aggtttacaa agaaatgcgc tggaatgtgc
    13981 aggatgtgag cagatttttg aagataaaat gagcggaaaa acaacacagc ggccgggact
    14041 gaagcgggca ctgaagaaac taaaacccgg tgacacgctg atggtctgga aactggacag
    14101 gctggggcgc agtatgcgcc accttgttgc gctgacagat gaattacagc aacaggggat
    14161 aaattttcgt agtcttactg acagcattga tacgtcaacc cccatggggc gcttcttttt
    14221 tcacataatg ggtgcgctgg cagaaatgga gcgtgaattg attgtcgaac ggacacgggc
    14281 tggtcttgca gctgcccgtg aaaggggacg aaccggcggc agaccccgtc gtctcacgca
    14341 ggaacaatac gagcaggtcg ggcgtttact ggccgggggt gtcagccgaa gacaggtggc
    14401 acttattttt gatatcggcc tttcaaccct ttatcgttat tttcctgcga gcgtttcgga
    14461 aaaataagac gctccctttg ttgggccttt actgaattaa cgcaaaatgg cgtttatcgg
    14521 tctgtggata tgataattct gtttattcag gaacaggatg aatattatga cgtccaaatg
    14581 ggttcaacta tcatcaatgc ccggaaactt taccgttaaa gtttccggtg gtacagcggc
    14641 atttcttgag gctccctttc ctccggctga aacgaaagga ggtatgacat ttgctgactg
    14701 tctcatcagt tttaatacac gggattgcct gtgggtaagg ccagtatccg gtgatccgaa
    14761 cgtggaaatt accggagcgg gtattggtgc tgtcattccg ttaagtgctg atgttgccgg
    14821 tactactgaa ccgtcagact ggaacaatgc ggaaacatac acttcttcag caggagataa
    14881 agttgcaggt caaactccgt cctggtatta cgtggttgtt attgctggtc agtcgaatgc
    14941 atcgtcattt ggtgaaggtc ttcctttgcc tgatacctat gatcgacctg atccacgtat
    15001 taaacagctg gctcgccgta acacggttac acctggcggg gttgaatgcg cctacaacga
    15061 tataattccg gcagatcact gtcttcatga tgtgctggat atgagtaacc ataatcatcc
    15121 aaaagcagat ctttcaaaag gtcagtatgg ttgtgtcggc cagggtttgc atattgcaaa
    15181 aaaactcctg ccctttatcc cggaagaggc tggtatcctt ctggttccct gtgccagagg
    15241 aggctctgca tttaccgatg gtgctgatgg tgaatttact gaagcttccg gagcaacatc
    15301 tgcatcatcg cgctggggag tcaataaacc tttgttcagc gatcttgtta accgaacaaa
    15361 agcggctctc agcagtaacc ccagaaatat tctcctgtcc gttgtctgga tgcagggcga
    15421 gaatgatctg aaaacaggaa aacatgctga acattccggt ctgtttgtga cgatggtcga
    15481 aacattcagg cgtgaacttt cggctttttc tgcgcaatct ccagccggag atatggcttc
    15541 tgtaccgtgg atctgtggtg atacgacgta ttactggaaa aatacatatc cagaacaata
    15601 caaagctgtt tatggcaaat atgctaatga agcaaagaac attcactttg ttccatttgt
    15661 tgtggatgaa aatggaaaaa atgtaccgac aaataatcca gatgaagacc cggatattcc
    15721 aggtattggc tattatggtg ccataagtcg cgattcgact aactggacat caaacgacag
    15781 ggcaagccat ttcagttcat gggcgcgccg tggaattatt tcagaccgtc tggcaacagc
    15841 gattctgaca ttgtcaggac aaaaatctgc tttcttccgc ggtgtaacgg caacagaaca
    15901 ttctgatacc ggtaagagtg agcctgaacc caccggggat gtgaaggacg tgagcgatca
    15961 gatgaagatc atcgtttcgt atactgcaaa cgccccgaca gaaccaatga aagatcaggg
    16021 gtggaatgcg gcttccggaa aagcaacctt tgtcacggat gctgctgcaa caggtggtaa
    16081 caaagcgtta agggtgaata aagcgcctaa cagcatggcc tcatggaaaa tgtatcatcc
    16141 gatcacagct gataatgcta gggatttgtt cgccagaggt ggtgatattt ccctccgttt
    16201 caaaattcca tcggatgcgg gcattgcgga aaaccggtat ggtgttggta tttactgggc
    16261 tgtaagtaac tggcctgacg gtgacggaaa aaataacctc atagcatcat gtttcctgca
    16321 aacggattca gctaacctga atgtgatgta tcacaaaggg agcggaagtc aacagatagg
    16381 atcgtatggt gtctttgatc atgaatggca tacgctctcg ttacggtttg caggtggtaa
    16441 tagcatgagt gttaccccag ttctggacgg gaaagatggt gaaccatttg aacttgtgaa
    16501 gtgggtgaat gccgggttca cggtggatca actgacactg accgatatta ccggcaatgc
    16561 agccacttac ccgatgttgt ttgacacaat taaagtgcag gttaacgatg cggtctcttc
    16621 gtctaatgtg aataaatgac gggtaattca ttttatttaa aatggtgagt gattatgcca
    16681 atactacata catttatgct ttatttttgc acgtgtgtgt gtctgcttta tctcctcagc
    16741 ggaggttaca aaattatccg aaattatttt cgaaataaaa ttgatgcagc cgcagaggaa
    16801 aagataaaaa gcggtgctgt aatgccgcag ccgaaagact ccggtcagat ttaatttttt
    16861 gtgaaccaga aaaggaaagg agaatattca tgccagagat taaaggcacg gttactgaag
    16921 agcttgtcaa acaggcactt tattctgaag aagtgaaccg cgtgctgaag gcgcaggttc
    16981 gcaaggattt tgaggcacaa atcgacgcat atgttgatga agtgctggcc cgaatggttg
    17041 gccatcctcc ggctgaaaac agcacggaga atgagcctca gcccgtagag caaccagagc
    17101 cggttcagcc cggaactgac agtgccatga tgtaacaata caaaccgaca gcttctcctg
    17161 tcggtttgat tttcatgggc gatgtatata ccagtcagag gaaataatca tggacaagaa
    17221 caaaaagcag cccgatacca aaggtctgga aatattaagc gcagaaatta tcaatgcgct
    17281 acgttcagga cttgatgaaa atacattaca ggatcgaaac ttcaccactg caatgtggat
    17341 agcgatgcag atcctgtgta ccccaagatg cgctattttt gcggttcgtg agggtcagtt
    17401 gactcgctga ttgcccgaat tacatcagaa aagcacgcgt tccataattg catagctgat
    17461 gaatcaccag ctgtatgaaa agaggtaaag acacaactgg tggatgtcat tgctgcaatt
    17521 gtgatatcag ctgcaattcg cgcagcctgt aacttttggt cggtggtcag tttatccatt
    17581 ttctcctgcc tgaacagatg catctctgct ttacaggagg ccggggaata tcataggata
    17641 caccacgagc tcagagtgaa gcattgatgc agttttttac agattgaccc agaatagttc
    17701 gcgctatctg agtcgccatt tatgtagtcc cgaagtaaag cattgatgca aacttgtcta
    17761 aatcggccca gcatagttcc agctatgctg ggtttccctt ttatgtagta tcctgttgat
    17821 ttgaaacata ctacatatac gcgcgatcct accttcactg gataaaaagg caataaaatg
    17881 atcattttat gatcgttttc accacctaac tcttcgtttt ctttaagtaa ataatcaccg
    17941 ccctgaatca ttgttgatga cttttcaatc tatttgttgt atcaattaaa caaatcgaat
    18001 cgatcgtttt tgtcgatcga ttgcatgaga aaggcatcaa caacaaagca ggaggttatg
    18061 cggccatgta tcgaaacata cggtgccgac actgcaataa gctactggca cgggccagtt
    18121 tcagttatct ggaagtaaaa tgtccgcgct gtaaaaccct caatcaaatc acatctccga
    18181 gcgccacaga gcaccccaca tacacaagga aatcctgtcg tggggaacag aaagcaagtc
    18241 acatcccgta tcatcagcac gcctgaactt attcgctata acgacaatat cgttggttat
    18301 ggctcccgtg agttgcgggt tgagacaata agttgctggc tggccagact ggtcatcgtc
    18361 aataaacatt acagccaccg tttcgtaaat aactcctacc ttcatctggg catattttca
    18421 gaacgggaac ttgttggcgt aatgcaatgg ggttacgccc ttaacccaaa cagtggtgca
    18481 cgcgtcgtaa cgggtacgca gaaccgagaa tatatggagc ttaaccggct atggctgcat
    18541 gactgtatgc cgagaaactc cgaatcaagg gcaatcagtt acgcgctcaa gctaatcagg
    18601 cagctttatc cgcaggtaca gtgggttcag tcgtttgcag atgagcgttg tggctgtctt
    18661 ggtgtcgtgt atcaggcaag caatttt
//
LOCUS       DAZFKF010000100        18631 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFKF010000100 DAZFKF010000000
VERSION     DAZFKF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.100"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..187
                     /locus_tag="ACHKA1_004056"
     CDS             <1..187
                     /locus_tag="ACHKA1_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II restriction endonuclease subunit R"
                     /protein_id="HFO5681631.1"
                     /translation="IKNDVVWLPVKNKSPDFCYINDLISVVHKLVIKDVVQYAERKMN
                     AYRQAITSRGYDDSGVS"
     gene            712..1659
                     /gene="stxA1a"
                     /locus_tag="ACHKA1_004057"
     CDS             712..1659
                     /gene="stxA1a"
                     /locus_tag="ACHKA1_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO5681632.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1669..1938
                     /gene="stxB1a"
                     /locus_tag="ACHKA1_004058"
     CDS             1669..1938
                     /gene="stxB1a"
                     /locus_tag="ACHKA1_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO5681633.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            2443..4356
                     /locus_tag="ACHKA1_004059"
     CDS             2443..4356
                     /locus_tag="ACHKA1_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5681634.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGDISSPAEPGSHPTVSTKPAAEPEYYYVIALAGQSNGMSYGEGLPLPDT
                     FDSPDPRIKQLARRSTVTPGGAACTYNDIIPADHCLHDVEDMTGLNHPKADLSKGQYG
                     CVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGTDGTYSDTTGASESSTRWGVD
                     KPLYKDLIGRTKAALAKNPKNVLLAVVWMQGEFDFSGTPANHTAQFGALVDKYRADLA
                     DMVGQCVGGSADGVPWICGDTTYFWKQKNETAYQTVYGSYKNKTEKNIHFVPFMTDEN
                     GVNVPTNKPEEDPDIPGIGYYGSKWRTDRSTWTSQDRASHFSSWARRGIISDRLATAI
                     LSCAGKSSAFVNGTAGVVVPDRPVTTSESVIFYDAKKASDNQLKPYGWDGMDGRRTLV
                     DDSGNKALRIEKNNSAKSWSMYCDIAADKAKLLLEKGGEIAVRFKIPENVNLETTRNK
                     YAFGLYWRIAEWPGEGGEGYLSSFFVQTDKASIDVAYHHTVNQQKELGTFGAFDHDWH
                     TLAFKFKGSNSINVTPVLDGVDGQAFDLVKWANTANGLNRFVITDITGSAETYPVLID
                     TVEVKANKAGAAA"
     gene            4496..4678
                     /locus_tag="ACHKA1_004060"
     CDS             4496..4678
                     /locus_tag="ACHKA1_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5681635.1"
                     /translation="MIFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWCRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            4704..4976
                     /locus_tag="ACHKA1_004061"
     CDS             4704..4976
                     /locus_tag="ACHKA1_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5681636.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            5053..5268
                     /locus_tag="ACHKA1_004062"
     CDS             5053..5268
                     /locus_tag="ACHKA1_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5681637.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            5270..5803
                     /locus_tag="ACHKA1_004063"
     CDS             5270..5803
                     /locus_tag="ACHKA1_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5681638.1"
                     /translation="MMSKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNYTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVAKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFSSTFYRKLNAGDRKGACAEIRRWIYDGGQDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            6077..6647
                     /locus_tag="ACHKA1_004064"
                     /pseudo
     CDS             6077..6647
                     /locus_tag="ACHKA1_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
     gene            6647..6796
                     /locus_tag="ACHKA1_004065"
     CDS             6647..6796
                     /locus_tag="ACHKA1_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681639.1"
                     /translation="MEWWLMISDKIITLTKTICVIVGVSFSLMMVALFLSIAWMALSS
                     VGLVG"
     gene            6847..6915
                     /locus_tag="ACHKA1_004066"
                     /pseudo
     CDS             6847..6915
                     /locus_tag="ACHKA1_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6917..7684
                     /locus_tag="ACHKA1_004067"
     CDS             6917..7684
                     /locus_tag="ACHKA1_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFO5681640.1"
                     /translation="MNNNSAKPFLPWLGGKRRLAKHILPLFPEHTLYVEPFCGGAALF
                     FMKKPSCAEIINDINSDIVNLYRVVKYHQEEFSRQFQWMLASREMWNEVINQHTEYMT
                     DIQRAVRFFFIQKCAYGGRAYSRAFGVKTHRPQIIPGNTEQLLQEARDRLARATIEHL
                     DWEVCIARYDRPTTLFYCDPPYWLDGGYGIDFSFCNYERMAELARTIKGKMIISLNDT
                     QEMRRLFEGMNIHHVAHRYTVPRGKTSSVSELIICNF"
     gene            7922..8107
                     /locus_tag="ACHKA1_004068"
     CDS             7922..8107
                     /locus_tag="ACHKA1_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO5681641.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKAPAPPAWIMQPA
                     PDWQTQLNGIISLSESG"
     gene            8464..9315
                     /locus_tag="ACHKA1_004069"
     CDS             8464..9315
                     /locus_tag="ACHKA1_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO5681642.1"
                     /translation="MAKLDWKKLEQAFRREHARTGIKLQDWCRKNNISYGTARRYIKL
                     RKNPPINTEESAQKNAQKSAQIDDQKSAQKNVQGSDDESSNDKAYSDDGSGEKCAKNC
                     ANSAETKRNSRSGNPHPVARFSDRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVF
                     TRARALSVTKALKGMFADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLT
                     LDVLAETAPKLRADRERINAARDKLRAETDILTSQRRGVITPVSDIVSSLHEMSNSGR
                     LDDIPEE"
     gene            9296..11002
                     /locus_tag="ACHKA1_004070"
     CDS             9296..11002
                     /locus_tag="ACHKA1_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO5681643.1"
                     /translation="MTFRKNKTRCDEPAEMTETEQSLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQEITASGLSLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYINKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPL
                     IVYDIEPVTGRKTKAQSLRDGNKAEQHRTLMNYLLVWELPDPDEEYVCGADIAEGLEH
                     GDRSSLDIIRCSNGEQVAHWFGHLDAELFAHLIAQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            11002..13146
                     /locus_tag="ACHKA1_004071"
     CDS             11002..13146
                     /locus_tag="ACHKA1_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO5681644.1"
                     /translation="MKNETNTMATKNDNGATPRFSQRQLQALCSDIDSQPKWRDAANK
                     ACAYYDGDQLPPEVLQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLVVMSDEPDD
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            13304..14311
                     /locus_tag="ACHKA1_004072"
     CDS             13304..14311
                     /locus_tag="ACHKA1_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681645.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQADKTDIRKTAEEKV
                     KTAEREQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            14335..15549
                     /locus_tag="ACHKA1_004073"
     CDS             14335..15549
                     /locus_tag="ACHKA1_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFO5681646.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            15605..15994
                     /locus_tag="ACHKA1_004074"
     CDS             15605..15994
                     /locus_tag="ACHKA1_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681647.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHVLIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            16044..16505
                     /locus_tag="ACHKA1_004075"
     CDS             16044..16505
                     /locus_tag="ACHKA1_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681648.1"
                     /translation="MSEKIAVVYIGPKTVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            16489..17052
                     /locus_tag="ACHKA1_004076"
     CDS             16489..17052
                     /locus_tag="ACHKA1_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681649.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFY"
     gene            17052..17702
                     /locus_tag="ACHKA1_004077"
     CDS             17052..17702
                     /locus_tag="ACHKA1_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFO5681650.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPVPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            17699..>18631
                     /locus_tag="ACHKA1_004078"
     CDS             17699..>18631
                     /locus_tag="ACHKA1_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFO5681651.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAITAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGKRGPQGIPGL"
BASE COUNT         4875 a         4132 c         5195 g         4429 t
ORIGIN      
        1 aattaagaac gatgttgttt ggttgccagt aaaaaataag tcacctgact tttgttatat
       61 aaatgacctt atttcagttg ttcataagct tgtcattaaa gatgttgtcc agtacgcaga
      121 acgcaaaatg aacgcataca ggcaagctat aacaagtcgc ggttatgatg attctggtgt
      181 cagttaaaaa acattttaca atcgtaaaaa tcctcatatc atgataagaa tggttacatt
      241 gccacgctgc ttaacccgcc gatgcgcggg ttttttgtat ccggaatcct gttagttata
      301 cggaaagtac acagaaagga aggtgcgacc acaattaata acaaaatctt aaaaatcgca
      361 catggcacta ttagttttct aaatattgtg tattttttgt attgcaggat gaccctgtaa
      421 cgaagtttgc gtaacagcat tttgctctac gagtttgcca gcccccccag tggctggctt
      481 ttttatgtcc gtaacatcct gtgtatcaat aaatgttgtt gtctacgtac gtcaagtagt
      541 cgcatgagat ctgaccagat atgttaaggt tgcagctctc tttgaatata attatcattt
      601 tcattacgtt attgttacgt ttatccggtg cgccgtaaaa cgccgtcctt cagggggtgg
      661 aggatgtcaa gaatatagtt atcgtatggt gctcaaggag tattgtgtaa tatgaaaata
      721 attattttta gagtgctaac ttttttcttt gttatctttt cagttaatgt ggttgcgaag
      781 gaatttacct tagacttctc gactgcaaag acgtatgtag attcgctgaa tgtcattcgc
      841 tctgcaatag gtactccatt acagactatt tcatcaggag gtacgtcttt actgatgatt
      901 gatagtggca caggggataa tttgtttgca gttgatgtca gagggataga tccagaggaa
      961 gggcggttta ataatctacg gcttattgtt gaacgaaata atttatatgt gacaggattt
     1021 gttaacagga caaataatgt tttttatcgc tttgctgatt tttcacatgt tacctttcca
     1081 ggtacaacag cggttacatt gtctggtgac agtagctata ccacgttaca gcgtgttgca
     1141 gggatcagtc gtacggggat gcagataaat cgccattcgt tgactacttc ttatctggat
     1201 ttaatgtcgc atagtggaac ctcactgacg cagtctgtgg caagagcgat gttacggttt
     1261 gttactgtga cagctgaagc tttacgtttt cggcaaatac agaggggatt tcgtacaaca
     1321 ctggatgatc tcagtgggcg ttcttatgta atgactgctg aagatgttga tcttacattg
     1381 aactggggaa ggttgagtag tgtcctgcct gactatcatg gacaagactc tgttcgtgta
     1441 ggaagaattt cttttggaag cattaatgca attctgggaa gcgtggcatt aatactgaat
     1501 tgtcatcatc atgcatcgcg agttgccaga atggcatctg atgagtttcc ttctatgtgt
     1561 ccggcagatg gaagagtccg tgggattacg cacaataaaa tattgtggga ttcatccact
     1621 ctgggggcaa ttctgatgcg cagaactatt agcagttgag ggggtaaaat gaaaaaaaca
     1681 ttattaatag ctgcatcgct ttcatttttt tcagcaagtg cgctggcgac gcctgattgt
     1741 gtaactggaa aggtggagta tacaaaatat aatgatgacg atacctttac agttaaagtg
     1801 ggtgataaag aattatttac caacagatgg aatcttcagt ctcttcttct cagtgcgcaa
     1861 attacgggga tgactgtaac cattaaaact aatgcctgtc ataatggagg gggattcagc
     1921 gaagttattt ttcgttgact cagaatagct cagtgaaaat agcaggcgga gattcataaa
     1981 tgttaaatac atctcaattc agtcagttgt tgccggtctg ataatagatg tgttagaaaa
     2041 tttctgcatg gtgaatcccc ctgtgcggag gggcgactgg tgaacggtat gatctctttg
     2101 atgatcgtaa gcgagaatac gcgggtttgg tggcaccaga ccgaactcac cgggaggcac
     2161 ccggcaccat gcagtattca gagattaggc atatatccag gctcctcatc gcaggagcct
     2221 ttttacatgc aaaaaaaaaa cgctcctggg aagagcggct ggcaagaaga aacaatatga
     2281 acaattaatt aacgatggga ataatacctt agagtaatca ccttgcgcaa ctgtaagggc
     2341 atatttcatc tttgcgggct gtttttctgt gtggcttctg tgtttccgga gggcagcctg
     2401 taccttttct gactcagaac attatcccgg ccgggaggat tcatggcatt taaacactac
     2461 gatgtggtca gggcggcatc gccgtcagac cttgctgaac gaataactca aaaactgaag
     2521 gaagggtggc agccttatgg tagtgcgctg atttcgacag ctggttatgg tgcagagttt
     2581 atccagccag ttgtgagtga gggggatatc tcatcacctg cggagccagg cagtcacccg
     2641 accgtttcaa cgaagcctgc agccgaacca gaatattact atgtgatcgc gcttgctggt
     2701 cagtccaatg gtatgtcata cggtgagggg ctgccattac cggatacatt cgacagccct
     2761 gatccacgaa ttaaacagtt agcgcgccgc agtacggtga caccgggagg tgctgcttgt
     2821 acctataacg atattatccc ggcagatcac tgtctccatg atgtggagga tatgacaggg
     2881 cttaatcatc ccaaagcgga tttatcgaaa ggtcagtacg gctgtgtggg acaggggctg
     2941 catatcgcca aaaagcttct gccatttata ccggcgaatg cgggcattct tcttgttccg
     3001 tgctgccgtg gtggttcagc tttcaccacc ggaactgatg gcacatacag tgacactact
     3061 ggcgcctcag agagttccac ccgctggggg gtggacaagc cgctgtataa ggacctcatt
     3121 ggtcgtacaa aagccgcgct ggcgaagaat ccgaaaaatg tgctgcttgc cgttgtgtgg
     3181 atgcaggggg agtttgattt cagcggcacg ccagcgaatc acacggctca gtttggcgca
     3241 ctggtggata aataccgtgc ggaccttgca gatatggtgg gacagtgtgt tggtggttct
     3301 gctgacggtg ttccctggat ttgtggagac acaacatatt tctggaagca gaagaacgaa
     3361 acggcgtacc agacggtgta cggtagttac aaaaacaaaa cggaaaagaa tatccatttc
     3421 gtgccgttca tgaccgatga gaacggggtg aatgtgccga cgaacaaacc ggaagaagac
     3481 ccggatattc cgggtatcgg gtattacggt tcaaaatggc gaacggacag aagcacctgg
     3541 acatctcagg acagggccag tcatttcagt tcatgggctc gccgtgggat tatttccgac
     3601 cgtctggcaa cggcgattct gagctgcgcg ggtaagtctt ctgcgtttgt taatggtact
     3661 gccggggtgg ttgttccaga cagaccggtt accacctcag agtctgtaat tttttacgat
     3721 gccaaaaaag cttcagacaa tcagctgaaa ccttatggct gggatggtat ggatggcaga
     3781 cgcacactgg ttgatgacag cggcaataaa gctctgcgaa ttgagaaaaa taacagcgcg
     3841 aaatcctggt caatgtactg tgatattgct gcagacaagg caaaactttt actggaaaaa
     3901 ggcggggaaa ttgctgtccg gtttaaaatc cccgaaaacg tcaatcttga gacaaccaga
     3961 aacaagtatg cctttggttt gtactggcga atagcggaat ggccgggtga gggtggtgaa
     4021 ggctatctga gttctttctt tgtccagaca gataaagcca gtattgatgt tgcataccat
     4081 catacagtta atcaacaaaa agaacttggc acgtttggcg cattcgacca tgactggcat
     4141 acgcttgcat ttaaatttaa gggcagtaac agcattaatg ttactccggt gcttgatggt
     4201 gtggatggac aggcgtttga cctggtgaaa tgggccaata ctgctaatgg actcaacagg
     4261 tttgtcatta cggatattac aggtagtgca gaaacctacc ctgtacttat tgatacggtg
     4321 gaagttaaag caaacaaagc tggagcagcc gcataattgc taaaaaaagc cgccagcggc
     4381 aggaatggaa gctggcggag gtaatcccaa tggagaatgt aaagaaaaga tgctttcgta
     4441 cattggtttt ttaaatgaaa acagttctca ttgtcaacca taacggtaag aaattatgat
     4501 atttattcat caggtgatgc tgtacttctg tacggcggtc tgtgtgctgt atcttctttc
     4561 gggtgggtac agggctgtgc gcgatttctg gtgcaggcag attgataaaa gggccgctga
     4621 gaaaatcagc gccagtcagt cagccggaag caaacccgaa gagcccgtta ctccttaaca
     4681 acccctttca gcgagaaaat cccatgtcag aaatcacatc cctggtcact gcagaggcag
     4741 tgaaggaagt cctgcgctct gaagaagtcc ggagcgcact gaaacagaaa cttcgccaga
     4801 atcttgaggc gcgtcttgat gcagaagttg atgccattct ggatgagctg cttggtgtac
     4861 aggcagagcc accgactgaa gcgggagata ccaccgcaga gagcggtgaa gttcagcctg
     4921 aatcaccggt cgccgatgcg actgaacctc aacccgaatc ggtcatgatg ctgtaacgag
     4981 gagtcagggc catcagtaaa cggctgctgg cctttttcat gttgtgagct tccggattgc
     5041 gggagacggg gtatgtacca gatggaaaaa atcacaacag gtgtgtcata caccacgtca
     5101 gcggtgggaa cgggctactg gttcctgcag ttgctggaca gggtttcccc gtctcagtgg
     5161 gcggcaatag gcgtgctggg gagtctgctg tttgggctgc tgacatacct gacgaacctg
     5221 tatttcaaga ttaaagaaga ccggcgtaag gcggcgcggg gagagtaagc tgatgagcaa
     5281 actccgctat ggtttatcgg ctgccgttct ggcgctgatt gctgcaggtg cttctgcgcc
     5341 tgaaatcctc gaccagtttc tggatgaaaa agaaggtaac tacaccacgg cataccgtga
     5401 tggtgcgggt atctggacca tctgccgggg tgccatcatg gtggatggta agcctgtgat
     5461 tcctggcatg aagctgtcga aggaaaaatg cgaccgggtt aacgctatcg aacgggataa
     5521 ggcgctggca tgggtggcga aaaacatcag agtgccactg accgaacctc agaaagcggg
     5581 gatcgcgtca ttctgccctt acaacattgg ccccggaaag tgtttcagct caacgtttta
     5641 ccggaaactg aacgccggag accgtaaagg cgcatgtgct gaaattcgtc gctggatata
     5701 tgacggtggt caggactgcc gtatccgctc aaataattgc tacggtcagg tatcccgtcg
     5761 cgaccaggag agtgcgctgg catgctgggg tatcgacagg tagcagaata ttttgctgaa
     5821 aaatgacgtt tgctcacgcg gacggataac acgaaatcct gcgaactggc aaaatgcaag
     5881 tgaataaagt caggaagatt gtttcacgca gaggcaccgt aatggtgtct ttgtcatttc
     5941 tgcgcttcgc acaagcgtaa ataaaccaaa gaacctttca ggatgagccc tggtggataa
     6001 ccggcagtgg tctggttaac cctctttggg ctggttattc ctgtgcgcag ggttcatcac
     6061 taaaaggaaa taaccgatga atatgatgac cgtgccgttt cacggcgatt ctctttatgt
     6121 ggttaaccat aacggtgagc catacgttcc catgaaacct gtcgttgcgg ggatggggct
     6181 ggcctggcaa tcacagttgg ctaagttaag acagcgtttt gcgtcaacta taacggaaat
     6241 cgttatggtt gctgaggatg ggaaacgacg caatatggtg tccctgccac ttcgaaaact
     6301 tgcaggctgg ttacaaacca tcaatcccaa caaagtaaaa cccgaaatcc gcgacaaggt
     6361 aatccagtat caggaagagt gcgacgatgt tctctatgaa tactggacga aggggggggg
     6421 tcgttaatcc ccgtcgaatg agtgtgatgg aagaactcaa tcaggcctgc gctgacatga
     6481 aacgggataa aaacattgcc agtgtgtttg ctaccgggct gaatgagtgg aaacaggtta
     6541 aagccgcgca tgtatcaaaa atccgcacat tgataaacga agcgaatctg ctgattgatt
     6601 ttgtcctggc tgatacaggc aaagggaaaa taacaaaggc ggattgatgg agtggtggct
     6661 aatgatatcg gataaaatca taacgctgac aaagaccatt tgtgtgattg tcggcgtttc
     6721 attttcgcta atgatggttg ctctttttct ttccatagcc tggatggcgt tgagttcggt
     6781 agggctggtg gggtgacagt gactgatgac gacaatttat ttaaacagat gagtactgaa
     6841 tttattcggg caacggcatt tgccattcgc attgtggcaa ttgcggttct aatccgggca
     6901 atccgctggt ggtgaaatga ataataactc tgcaaaaccg tttctcccgt ggttaggagg
     6961 gaagcgtcgt ctggcaaaac atattctgcc gttatttcct gagcacactc tgtatgtgga
     7021 accgttctgt ggcggtgcgg cactgttctt tatgaaaaaa ccgagctgcg ctgaaatcat
     7081 caacgatata aacagtgata tcgttaatct ctaccgtgtg gtgaagtacc accaggagga
     7141 attttcccgc cagtttcagt ggatgctcgc cagtcgtgag atgtggaatg aggtgataaa
     7201 tcagcacacg gagtacatga cggatatcca gcgggctgtc cgtttctttt ttatccagaa
     7261 gtgcgcgtat ggcggaagag catacagtcg ggcatttgga gttaaaactc acagaccgca
     7321 gataatcccc gggaatacag agcagttatt gcaggaagcc cgtgaccggc tggcgcgggc
     7381 aactattgaa caccttgact gggaggtctg tatcgcacgg tatgatcgcc cgacaacgct
     7441 gttttactgc gatcctccgt attggttgga tggtggttac ggcattgatt tttcattctg
     7501 caattatgag cgcatggcag aacttgcccg cacaataaaa ggcaaaatga ttatatccct
     7561 gaacgatacg caggaaatga gacgattgtt tgaggggatg aatattcatc atgttgctca
     7621 tcgttatact gtaccacgtg gtaagacaag cagtgtcagc gagctgataa tctgcaattt
     7681 ctgacagggg gcatggtgag cataaaccga atgctttccg cgtttaccgt tattctgctg
     7741 gtagcctgtg gtgtgcttag tctggggctg aatcattacc gtgacaacgc gctcacctac
     7801 aaagcgcagc gcgataaaaa agccagagag ctggagctgg caaacgcaac cattactgat
     7861 atgcagcagc gccagcgcga tgttgctgcg ctcgatgcaa aatactcgag ggaattagcc
     7921 gatgcgagag ctgaaaatga aactctgcgt gctgatgttg ccgctggtcg taagcgcctg
     7981 cggatcaacg ccacctgctc cggtaccgtg cgtgaaggca ccggcacctc cggcgtggat
     8041 aatgcaaccg gcccccgact ggcagacaca gttgaacggg attatttcac tctcagagag
     8101 cggttgatga tgatgcagaa gcagctggaa ggggcacagg actatatccg cactcagtgc
     8161 ctgaaataag ttttgctgat gcgccgtatc gtcgctgtat tccctcatta acagagactg
     8221 cagccacctt atctgcgtga gtgtgcggga ataatcaaaa acgatgcaca ccgggttttc
     8281 tcatttgtca cgagatgtga gcgattcccc gcgaagctgc ccgtccggtg cggtggtgga
     8341 agaaaccgga taaaacaact gctttgaaca aatattggtc aaataaggcg ctgctgtgtg
     8401 aattctgaaa aatcacagcg gtcattatgc gtcagttttt aacagaggac gtcagaaagt
     8461 gacatggcaa agctggactg gaaaaagctg gagcaggcat tccgacgcga acatgccaga
     8521 actggcataa aacttcagga ctggtgtcgt aagaacaata tcagttatgg cacggctcgc
     8581 cggtacataa aattgcgcaa aaaccccccc ataaatacag aggaaagtgc gcagaaaaat
     8641 gcgcaaaaaa gtgcgcaaat tgatgatcaa aaaagcgcac aaaaaaatgt gcagggatcc
     8701 gatgacgaat ccagtaatga caaggcttac agcgatgatg gcagtggtga aaaatgcgca
     8761 aaaaactgcg caaattcggc agaaacgaaa cgcaacagca gatcgggcaa tcctcacccc
     8821 gttgcccggt ttagcgaccg taacacccac gccgtaagac accgtggata tgcgaagtat
     8881 cttgaggcag ataacctcat ggatgatgcg tccgacatgg tgctgttcga tgaactggtg
     8941 ttcacccggg cccgcgcact ttcagtaact aaggcactta aagggatgtt cgccgacctg
     9001 gaagaggcaa ctgacgtgga aacccgcgtt gctctttacg acaaaatact caaagctgaa
     9061 caggcccttg accggaatat tgcccgtatc gagtcaattg aacgctcatt gctgacgctg
     9121 gacgtcctgg ctgagacagc accaaaactt cgtgctgacc gggaaagaat caacgccgcc
     9181 agagataaac tcagagcgga aaccgatatt ctgaccagcc agcgccgggg tgttattacg
     9241 ccagtcagtg acatcgtgtc atcgctgcat gaaatgagta attcggggag actggatgac
     9301 attccggaag aataaaacgc gatgtgatga acctgcagaa atgactgaga ccgaacaaag
     9361 tctgttcatt atgacaaaac tgagcaatcc ctggtggcgc ctcaatcatc tctacaaaat
     9421 acagaacgaa aaaggtgaac tggtcacctt cagaatgcga ccggcgcaac gtcagttgtt
     9481 tcgtaacatg cataataaaa acattatcct gaaagcgcgc cagctgggat tttccacagc
     9541 cattgatatt tatcttctcg accaggcatt attcattccg catctcaaat gcgggatcgt
     9601 cgctcaggat aaacaggctg ccagtgaaat tttccgcaca aaaattgctg taccgtttga
     9661 tcatctccct gactggctga gagcctcatt caccatcgtt gaacgtcgta gcggtgccag
     9721 cggtggctat atcctgtttg gtcacggctc gagtatccag gtggcaacct cattccgttc
     9781 aggtacggtg cagcgcctgc atatctcaga gcacggcaaa atttgcgcga aatatccggc
     9841 taaggcgaaa gaactgcgaa ccggtacgct taatgccgtc tctgatgaat gcattatttt
     9901 tgatgagtcc acagctgaag gcgtgggtgg tgatttttac gagatgagta accgagcaca
     9961 ggagatcaca gcatcaggct tatcgttgac ggcacaggat tataaattcc atttttacgc
    10021 ctggtggcag gatcctaaat acagcgcccg agtgccggaa agcgggctga agctgtcacg
    10081 ggaaaaaatg acgtattttt ctgcggttga gaaggcaatg aacatcacgc ttactgatga
    10141 acagaagcag tggtacatca ataaggaaac tgaacagcgt gaggaaatga agcaggagtt
    10201 tccctcaacg ccacaggagg cgtttctgac gtccggacga cgtgtgttca gtgccgaaag
    10261 tacgttgcag gcagaatcat tctgttcgcc accgctgatt gtttatgaca ttgaacctgt
    10321 tacaggaagg aagactaaag cgcagtctct gcgtgacggg aataaagccg aacagcaccg
    10381 gacgctgatg aattatctgc tggtctggga gctaccggat ccagatgaag agtatgtctg
    10441 cggagcggat attgctgagg ggcttgagca cggggaccgc tcatcgctgg atatcatcag
    10501 atgcagtaat ggtgagcagg tggcccactg gtttggtcat cttgatgcgg agctttttgc
    10561 tcatctcatt gcgcaggtct gtcgtatgta caacaacgcg tttgtggggc cggagcgtaa
    10621 caatcacgga catgccgtta ttctgaaact cagggagctc tatccgacgc gttatatcta
    10681 caacgaacag caccttgatc aggcatatga tgacgatacg ccccgccttg gctggctgac
    10741 aacccgtcag agcaaacctg ttctgaccga aggaatgaaa acgctcctga ataatggaat
    10801 atcagggatc cgctggtcag gcacattatc ggaaatgaac acctacgttt atgacgcgaa
    10861 aggctccatg aatgcacagg aaggctgttt tgatgatcaa ctcatgagct acatgattgc
    10921 ccaggagatg cgcgccagaa tgcctgtgag ggtaaaacag aaaacggata aacgcagaac
    10981 tacacactgg atggctcact gatgaaaaat gaaactaaca ccatggcgac gaaaaacgac
    11041 aatggagcca cgccgcgttt ttctcagcgc cagttacagg cgctttgttc tgatattgac
    11101 agccagccta aatggcgtga tgccgcaaac aaggcctgtg cgtattacga tggcgatcag
    11161 ttgccaccgg aagttcttca ggttctgaaa gatcgcggtc agccgatgac tatccataac
    11221 ctcatcgcgc ctaccgtcga tggcgttctg ggaatggagg ccaaaacacg gactgatctg
    11281 gtggtgatgt cagacgagcc agatgatgaa actgaaaaac tggctgaagc tattaatgct
    11341 gaatttgccg atgcatgccg ccttggcaat atgaataaag cccgctctga tgcctatgcg
    11401 gaacaaatca aggcgggcct cagttgggtg gaggtcaggc gcaacagcga tccgttcgga
    11461 cctgaattca gggtatccac agtcagcaga aatgaggttt tctgggactg gcttagccgg
    11521 gaatccgacc tgagtgactg ccggtggctg atgcgtcgcc gctggatgga taccgatgag
    11581 gcaaaagcca cgttcccggg aatggctcag gttatcgatt atgctattga tgactggcgt
    11641 ggttttgtcg ataccacgat tactgaaggc cagcccagtc cgttgatgag tgcatgggaa
    11701 gagtatcagt catgggatcg cagggagaat gaatggcttc agcgtgaacg ccgtcgtgtg
    11761 ctgcttcagg tggtttatta ccgtacattc gagcgtcttc cggtgattga actcagtaat
    11821 ggacgggtgg tggcttttga taaaaataat ctgatgcagg cggtggctgt ggcatccggg
    11881 cgggttcagg tgaaagtcgg gcgggtaagt cgtattcgtg aagcctggtt tgtgggccca
    11941 cactttattg tggatcgccc ctgtagtgca ccgcagggga tgtttccgct ggttcctttc
    12001 tggggatacc gaaaggataa aaccggggag ccatacgggt taatttcccg cgccattccg
    12061 gcacaggatg aggtgaattt tcgtcgtatc aagctgacct ggctgcttca ggccaaacgc
    12121 gtgattatgg acgaggatgc cacccagttg tcagacagcg acctgatgga gcagatcgaa
    12181 cgtccggatg gcattattaa actgaatccg gcccgaaaaa atcagaaaag tgtcgcagat
    12241 gtttttcggg ttgagcagga ttttcaggtt gccagccagc agtttcaggt catgcaggaa
    12301 tcggaaaaac ttatccagga taccatggga gtgtattctg catttctcgg gcaggattca
    12361 ggtgcgacgt caggcgtggc tatcagtaac ctggtggagc agggggccac aacccttgcg
    12421 gaaatcaacg ataactacca gtttgcctgc cagcaggtgg gaagactgtt gctggcttat
    12481 cttctcgatg acctgaaaaa acgccgtaat catgcagtgg tgattaatcg cgatgatcgc
    12541 cagcgtcgcc agaccattgt cctcaatgct gaaggtgata atggtgaact gaccaatgat
    12601 atttcaaggt taaatacaca tattgcgctg gcgcctgttc agcagacacc ggcgtttaag
    12661 gcacagcttg cacagagaat gtcagaggtt attcaggggc tgccgcctca ggtgcaggct
    12721 gttgtgctcg acctgtgggt taatcttctg gatgtgccgc agaaacagga gtttgttgag
    12781 cgtattcgtg ctgcgctggg gacgccaaaa tcaccggatg aaatgacgcc ggaagaacag
    12841 gaagtagcgg cacaacaaca ggcacttcag caacaacagg cagaactcca gatgcgcgag
    12901 atggctggca gagtggcaaa actggaagct gacgccgcca gggcacatgc agctgcacaa
    12961 cgggataatg ccagtgcaca gcgggaagtc gccctgacac aggggcagcg ttatgtggat
    13021 gcgcttaacc aggcacatac ggcagaaatc attaccggcg tacagaatat ggaacaggag
    13081 caggacgttc ttcagcaaca gatgctgtat acgttacaac agcggatgaa tgaaatgtcg
    13141 ctctgaaaac tctggcttca actgaacccc gtcatcgtac ggggtttttt gtttccggag
    13201 gtaagcgttc cgggagcggt gcgcttattc gcgggggcag cgataagcct tatttactca
    13261 accattcgga tctgtccgat aaacagacca tgcggagtta tttatggatt ttgaatttac
    13321 gggtgaagaa accccggaac aactggaaaa aatgctggaa ggacttgggg atgtggatat
    13381 tgacagtcac gcacaggacg tcgtgacgga agataccacg gaaaaacatg cggatgagga
    13441 agcacagact cagacgggcg ataacaatgt ggcaccgacg ccggatgcca gtgtggagca
    13501 gacgcaggac gtgaaggagc cggaagcgaa gggggtgctc acccgcgacg gtaaacacgt
    13561 cattccctat gaagtccttg aggctgaacg ttccggtaag caacgggccg aacaggaagc
    13621 cgcacttctt cgtgggcaga tagctgaaga aaaacgcagg gtggaactgc tgacgtctca
    13681 gatccaccag gccggtatga agcccacacc gttaccggaa aacgaaaaaa tttctgatga
    13741 gcagattgcc cgtatcaggg agatgtatcc ggaaattggt gacgcggtgg cttcgctcat
    13801 ccgtaaaaat aactatctcc agtcccgtgt tcagcaatca acacagcagg cagaaggtaa
    13861 tggtggtgag gatttatcac cggttcttga tgcgatgaat gccgtgccgg tgctgaaaac
    13921 gtggcaggag tccgatccgg atcgcttctc ggttgctgta tccatcgacg ggaagctcca
    13981 gaatgacccc gcatggaaag acaaaacgct cactgaacgt ttcgctgaag tggcccgtcg
    14041 tacgcaggtt gctttcggtg aagtcagtga gtcgtctgct gacaaccagg cagacaaaac
    14101 ggatatccgg aaaacggcgg aagagaaagt gaagaccgct gaacgggagc aggcagtacc
    14161 agcttccccg tcagatttag gcaccacggc ctccgtcgga accggtgata attttgaacg
    14221 gttacttggc gcttcgcatt cagaggcaga ggcgattatg cgcggtatga cgaatgctga
    14281 aatagacgca cttctggaga agctcgggta acttactgaa ggagtactga agtaatgacg
    14341 actgtaacat cagcccaggc gaataagctg tatcaggtgg cgctttttac tgctgccaac
    14401 cgcaaccgct cgatggttaa tatccttact gaacagcagg aagcgccaaa agcggtttcg
    14461 ccggacaaga aaagcacgaa gcagaccagc gcaggtgcgc cggttgtccg tatcacagac
    14521 cttaacaaac aggccggtga tgaagtgacc ttcagcatca tgcacaaact ctcaaaacgt
    14581 ccgacgatgg gagatgagcg tgttgaaggt cgtggtgagg atctcagcca tgctgacttc
    14641 tccctgaaaa tcaatcaggg acgtcacctg gtggatgcag gcggacgtat gagtcagcag
    14701 cgcacgaagt ttaacctggc atcctctgcc agaacgcttc tggggacgta ctttaatgac
    14761 ctgcaggacc agtgtgcgat agtgcatctt gcgggagctc gtggtgattt tgttgctgac
    14821 gacaccattc tgccgacagc ggagcaccct gaattcaaaa aaatcatgat caacgatgta
    14881 ctgcctccga cacatgaccg tcactttttt ggcggtgatg cgacaagctt tgagcagatt
    14941 gaagcggcag atattttttc tattggcctg gtggacaatc tctccctgtt cattgacgaa
    15001 atggcgcatc cgttacagcc ggttcgtctg tccggtgatg aacttcacgg agaagatcca
    15061 tattacgtcc tgtacgtcac gccgcgtcag tggaatgact ggtacacctc gacgtccggt
    15121 aaggactgga accagatgat ggttcgtgcc gtgaaccgtg caaaaggttt taatcatccg
    15181 ctgttcaaag gtgaatgtgc gatgtggcgc aatatcctgg ttcgtaagta tgcgggtatg
    15241 ccgatccgtt tctatcaggg gtcaaaggtt ctggtatcag agaataacct gacggcaacc
    15301 acgaaagagg tcgctgctgc aaccaatatt gaccgcgcca tgttactggg ggctcaggcg
    15361 ctggcaaatg cttacggtca gaaggcgggc ggtcacttca acatggttga gaagaaaacg
    15421 gatatggata accgtactga gatagcaata agctggatca acggtctgaa aaaaatccgt
    15481 ttccccgaga agagcggcaa gatgcaggat cacggcgtga ttgccgttga tacagcagtg
    15541 aagctctgat tttttccttt ccccatgccg ggttttcgcc cggctttttc aggagtcatt
    15601 aattatggca aagactatcc ttgccccgtc actgagtgaa cgggtctata cgggtacgca
    15661 cggtaatgag tcggtggcag aaggcgtatt tacggtgaat gctgcggaag cggacagtgt
    15721 tattcatctt ctctcactgc cagtgggcat ccgtatcaac tcactccagc tggtttcaac
    15781 gggtggtctg ggtactgcaa ccgtcagcat taagtccggt gagcatgttc tcatcgataa
    15841 cagcgaagct gtttctgcaa aatttgccag atatgtgcca gtggagccgt acaccacaca
    15901 gcgtgacggg gagctggtta ctgtcaccat taagactgcc gctgcaaccg gcaccctgaa
    15961 tgttctgctg cgttataccg tggtgggata ctgattaaaa ccttccggcc cgcgtcatgc
    16021 gggcttttta tccggggaat tatatgagtg agaaaattgc cgttgtctat atcggcccaa
    16081 aaaccgtgaa aaaggacacc attaccggaa gccgcacatt gttcccacgt cttgagccgg
    16141 tgcatgttga cagtgcgatg gcctggcaac tgctggggtt tccggatgtc tgggttcgtc
    16201 atgaagagct tgatgatgtt ctgaaaaagc aacaacagaa tgagcagttg cggcaggcac
    16261 agcaggcgca ggaaagagtg cttgctgcgc gggcagaagc ggagaacagt tttgttgttt
    16321 ctgttaacgg gcaggaggtg gatttaagta agctcacctc agcacggctg gcgacgctgt
    16381 gtgaggcaga agagctggat attcacaaag acccgaaaga aacggctgag gcattccgta
    16441 tccgggtgcg tgaggcattt cgccgtcgtg ttgcggagac tgaacagcat ggcggaactg
    16501 agtgattttt taccgtatgt ccgtcgtcat atcagcggtc cactgaacat tatgatgacg
    16561 gatgctctgt caatggctgc tgtggcattc agccgccagt cgttggtgtg ccgtcgggag
    16621 gttactgttg taccagtagc aggaaaagaa atcgtgcttc cgtatgacaa agatgatgag
    16681 gagtgcgttc atatcatccg tatctctgac gataatcatg agctttttgt cggtcgggat
    16741 gtggatatca gctccggacg ctccctgcga tttgcctgtt ctcccggtga ggtgagcgtg
    16801 ctttatgccg tcgctccgaa agccggacgc agccagatac cggatgaact cctcacatgg
    16861 cctgaagaag tggctgcggg ggcacttgag cggttgttca tgcagactgg tgtttcatgg
    16921 tcagatccgt tacgcgcaca gtatttttct gtgcagtttt ctgaggggat ccgtcgggca
    16981 tatcgtcata cactggcgac aagcccgtac tcttcatacc gcaaccctgt acgcaggcag
    17041 aggttttact gatgacgacg attacagaaa tcatcggacg tgtgaataca caactggttg
    17101 acccgatgat ggttcgctgg cccctgcagg aattgtgcga ttattacaat gatgctgtga
    17161 gggcagtgat tctggcgaga ccggatgctg gcgcaagcct ggaaacaatc agttgtgttc
    17221 ctggcgcccg tcaggttttg cccgatggtg taatacaact tcttgacgtg atatgcctca
    17281 gtgacggtag tgcagtcaga ccattatccc gggaggtgct ggatgcgcag tatcctgagt
    17341 ggcccacaat gaagggcatt cctgaatgtt ttatcagcaa cgacctgtcc ccgcgcgtat
    17401 tctggctgtt tcctgtccct gacaaagaga taagtattga tgcagtggta agccggatac
    17461 cggaggcagt gtatgttctg acgcaggacg atgatacgcc agttccactg gaagaggctt
    17521 atgttaaccc actggtggac tggatgttgt ttcgtgcttt cagtaaggat gctgccggtg
    17581 gtgcagaatc ggggctggct gcgcagcatt atcagagttt tgttgagcaa cttgggatca
    17641 aacagggggc agacagtgca ttgtctgccc gtaaaaaagt gtttaacgga ggtggagtgt
    17701 gagtgttgtt gtttcgggga cgctgaaatc tcctgatggt gaggcgatat caggagcaaa
    17761 tattaccctg acggcgctga cagtttcacc ggatgcgctc agcggcacca gtgcgtcggc
    17821 agtgacccgt gaaggtggat attacggaat gacgatggat ccgggggagt atgcggtttc
    17881 ggtgacggtg aaagggaaga ctgctgtcta cggacgtgtg cgtattgagg ggaccgaaag
    17941 tacggtgacg ctcaatatgc tgttacgccg cagtcttgtt gaggtgagca tacccggaga
    18001 gctgctgaca gatttccggc agatacagaa taatgtggct gatgaccttg ctactattcg
    18061 tcgcctgaat gaagacacgg cgacaaaaaa cactcaggcc acacagtcaa aagaaagtgc
    18121 agcagccagt gcgaagagtg catctgacag tgcaaagacg gcaaccagca gggcggctga
    18181 agccggacaa aaagcgactg atgccactga ggccgcgacc cgtgcaatca cagcagcggg
    18241 gaatgcagag gaaagctcga cccgtgccgg agagtctgaa aaagccgccg gagctgatgc
    18301 agaaaaagcc agacagcatg ctgaaaaggc caggctggcg caggagagcg ccggagagat
    18361 ccttaagcgg gcagaggctg ccactgtcag tgctgaagag gccagacgta tggctgagaa
    18421 tgcacggggg ccccgggggc ctcagggaga aactggtccg aagggggatg tcggtcctaa
    18481 aggcgaaaca ggtccagtgg gccctcaagg gcccgcaggg ccgaaaggtg agcgtggtga
    18541 cgttggtgct cagggggctg tagggcccgc tggtccgcgt ggtgagaagg gcgaacaggg
    18601 gaagcgagga ccgcagggaa taccaggcct g
//
LOCUS       DAZFKF010000101        17844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFKF010000101 DAZFKF010000000
VERSION     DAZFKF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.101"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            748..2091
                     /locus_tag="ACHKA1_004079"
     CDS             748..2091
                     /locus_tag="ACHKA1_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681652.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVTPLVPEEVHLLAVAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NAANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNALQGVSGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGG
                     AEKWSKKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(2240..3748)
                     /gene="putP"
                     /locus_tag="ACHKA1_004080"
     CDS             complement(2240..3748)
                     /gene="putP"
                     /locus_tag="ACHKA1_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO5681653.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(3907..4116)
                     /locus_tag="ACHKA1_004081"
     CDS             complement(3907..4116)
                     /locus_tag="ACHKA1_004081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681654.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            4171..8133
                     /gene="putA"
                     /locus_tag="ACHKA1_004082"
     CDS             4171..8133
                     /gene="putA"
                     /locus_tag="ACHKA1_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO5681655.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQTVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVELALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENNEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(8173..8811)
                     /gene="rutR"
                     /locus_tag="ACHKA1_004083"
     CDS             complement(8173..8811)
                     /gene="rutR"
                     /locus_tag="ACHKA1_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO5681656.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            9099..10190
                     /gene="rutA"
                     /locus_tag="ACHKA1_004084"
     CDS             9099..10190
                     /gene="rutA"
                     /locus_tag="ACHKA1_004084"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO5681657.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGYFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            10190..10882
                     /gene="rutB"
                     /locus_tag="ACHKA1_004085"
     CDS             10190..10882
                     /gene="rutB"
                     /locus_tag="ACHKA1_004085"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO5681658.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            10894..11280
                     /gene="rutC"
                     /locus_tag="ACHKA1_004086"
     CDS             10894..11280
                     /gene="rutC"
                     /locus_tag="ACHKA1_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO5681659.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            11288..12088
                     /gene="rutD"
                     /locus_tag="ACHKA1_004087"
     CDS             11288..12088
                     /gene="rutD"
                     /locus_tag="ACHKA1_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO5681660.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            12098..12688
                     /gene="rutE"
                     /locus_tag="ACHKA1_004088"
     CDS             12098..12688
                     /gene="rutE"
                     /locus_tag="ACHKA1_004088"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO5681661.1"
                     /translation="MNEAVSPGALSTLFTNARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            12699..13193
                     /gene="rutF"
                     /locus_tag="ACHKA1_004089"
     CDS             12699..13193
                     /gene="rutF"
                     /locus_tag="ACHKA1_004089"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO5681662.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            13214..14542
                     /gene="rutG"
                     /locus_tag="ACHKA1_004090"
     CDS             13214..14542
                     /gene="rutG"
                     /locus_tag="ACHKA1_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO5681663.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAIMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(14625..14798)
                     /gene="ymdF"
                     /locus_tag="ACHKA1_004091"
     CDS             complement(14625..14798)
                     /gene="ymdF"
                     /locus_tag="ACHKA1_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO5681664.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            15171..15767
                     /gene="wrbA"
                     /locus_tag="ACHKA1_004092"
     CDS             15171..15767
                     /gene="wrbA"
                     /locus_tag="ACHKA1_004092"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO5681665.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            15788..16015
                     /gene="yccJ"
                     /locus_tag="ACHKA1_004093"
     CDS             15788..16015
                     /gene="yccJ"
                     /locus_tag="ACHKA1_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO5681666.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(16053..17294)
                     /gene="agp"
                     /locus_tag="ACHKA1_004094"
     CDS             complement(16053..17294)
                     /gene="agp"
                     /locus_tag="ACHKA1_004094"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO5681667.1"
                     /translation="MNKTLITATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(17587..>17844)
                     /locus_tag="ACHKA1_004095"
     CDS             complement(17587..>17844)
                     /locus_tag="ACHKA1_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681668.1"
                     /translation="INEVKNFLTALSDSICSLFIVNSNVASISLGNESFSTDEDLEYG
                     YLINTGNHYDVYLPPELFAQAYKLNNKEMNAQLDYLNRYAI"
BASE COUNT         4336 a         4678 c         4633 g         4197 t
ORIGIN      
        1 caaaaaatta taatccccct tattatattt ccagtgatat cattttatgg ctaaagctat
       61 atacaactct aaatcacgat aaaacgaagc aggatgcgca ttaaaaaagt cacacataat
      121 aaaaatagaa atcttaacca gaagtaatag atcatcgtga aagtcaatca tcaaaaacac
      181 caacacacaa aaatcaataa gttaataatt gttcagggct tttttctcac gatttttcta
      241 ctatgacatt atcacaaacc cactagcatg aatacagaaa gagaagtaat aagatgagac
      301 gccactgctt tcagatgaac cctgtaatta gggtcgagaa tatagtttat ccattcccct
      361 atcgataaat taaaaaattt gcgtattaat ttttctctga ctgaaggcta acgtcgaatt
      421 accgacaaga ttcacgagtg attgtaacta cgtactttat gaaacacagg aagaaattac
      481 tatttaccgc gatagttcaa ggcgtaaaaa aaataataat attcatgaaa ctcatcccga
      541 tggcagcatt gtaaattcgc caataactgg cattcctcct gctttttacg ggtgtttcca
      601 ttaacaccag aatgagcgga ttgtttacta catcaagaac gcatccgaag tgtacatcct
      661 ggtgcgccga ctgaatcgcc cggagccatg cggatggcta cgacttaata cgaatcatta
      721 accctgtaag gatacggaga gcatgacatg agaccaatat ccaacctcaa tacgagcgca
      781 gcaacctata ttccaccgca acaactcccg agtagttgtc tggaaacttt gacgcttctg
      841 gtgcagatga ataattatat caaaagggat gcggactaca gcacgggcat ggctgttact
      901 ccactggttc ctgaagaggt tcacttgctg gctgttgcga tgacaaccga actacatcga
      961 catcagttcc aaccggtggt aagtgcaaat gatctgcaac ttcctgaacc gttcaccttc
     1021 gatgttgccg gtttcaccat caccttcacg aagacgcaag agcatgacaa cacgggcaat
     1081 ctcatgaaaa ttgcagtcag caagagcggt gtgtgcacca gtacaaatat tactcttgag
     1141 ctgtttcact ccattgtcac aaccctgatg tcacgcagtc aatatggaac ctttgatctc
     1201 tggtccgtac ggccaatact cacggatgaa agtcaaaaca gggtgcatga agcagctcgc
     1261 tactccccgg cccagcaata cggaagagaa gaaacacact ttaccaacaa tggcatgcga
     1321 gaatttggaa atatgtctcc gctggcgtgg cgtaacgatg tggaattaca gcaatcgtgt
     1381 aatacaagca acatcccacc aaatgctgca aatgacgaca taaacaacac caggcagacc
     1441 atcgaggacc agccagaggc ggacgagcca cagcaatatg ttaaattgac tgtagacgac
     1501 atgcgaaaat gggcggctat ggaccagcag gcgcgaaacg ctctacaggg agtttcagga
     1561 tggtgtacgc gcaatcattt taatatcaaa aatgccagaa attacctgac cgatcacggc
     1621 ctgaactacg cgggacaggt aaaggtaaac aggccgcatg aatatgccaa attcaccctt
     1681 gaacatatcc gccagtgggc agccttaaac aagcatgtgc gaaaaccggt tggttattta
     1741 gaaaagtggt gtaaggaaag aaacctcgcg ccaactactg ccaggaatta cctgaagaac
     1801 gacgggctta ccgccctggg tgagttgaag ctgacagggc cgcagaaatg ggtcacgttc
     1861 accttcggag acatcgtaca atgggcgaat atgacccagg aggagcgtaa cagcgcaggg
     1921 ggagccgaaa agtggagtaa aaaacgcggc ttccagtggt ctactgccag atcctacctg
     1981 aaatcgagcg gagtgaccaa gcaaggtgcg agaaaactgg cgtggttaaa aaacagcggg
     2041 aatatgtcga atccattcta tctcgcccaa ccaaccagcc gcaggacata agctgaaggt
     2101 accgatagcg gcaattttgt tcatcagtgc cgggtgggca aatagcaatt gcccccgaac
     2161 actccctgcc aggcaactta taccgcatgg cagggaacat tcgggttaaa aagcaaaaga
     2221 taaataatta ctcttcagct tagccttcct gcaaccgtga cggcggtgcc gaatgatagt
     2281 gcgcatcggc ctcggcaaag cgtttttgca tcgccgctga cggcgcttta cccagcaaac
     2341 taaacactac aatcccaata ctgccgaaga taaagcccgg aataatttcg tacagtccca
     2401 gccagccgaa ctgtttccag acgataaccg tcagcgcccc gatgatcatc cccgccagcg
     2461 caccgttgcg cgtcatgcgt gaccacatca ccgagaacag caccaccgga ccaaacgccg
     2521 cgccaaagcc tgcccacgcg tagctcacta agcccagcac gcggttttcc gggtttgccg
     2581 ccagcgcaat ggccaccagc gccaccacca gcaccatcac acgccctacc cacaccagct
     2641 ctttctggct ggcatgttta cgcagaaacg ctttatatag atcttcggta atcgcactgg
     2701 aacataccag cagctggcaa cttaaggttg acattaccgc cgccagaatt gccgacagca
     2761 gaatcccggc aatccacggg ttaaacagaa tttgcgccag ttcgataaac acgcgttcag
     2821 cgttctggtt taccgcacct gctaccgccg ggtgctcgtt aaagtaagcg ataccaaaga
     2881 agccgacagc caccgcccct gccaggcaga ggatcatcca ggtcatactg atacgacgcg
     2941 catggacgat gctgtggtga gaatccgccg ccataaaacg cgccaggatg tgcggctgcc
     3001 cgaagtaacc cagcccccat cccatcagcg agataatggc aacaaagttc agccctttga
     3061 gcatatccac gttttcgatg ctcttttgtt tgatcacttc cagagagtcg ccaaagccac
     3121 cgacactgat aatgacgata accggcgtca ggatcagggc aaaaatcatc aggctggcct
     3181 gtacagtatc agtccagctc accgcgagga aaccgccaat aaaggtgtaa aggatcgtcg
     3241 ccgcagctcc cgcccacaga gccgtttcgt agctcatgcc aaaggtactt tcaaacagac
     3301 gcgcgcctgc cacaatgccc gaagcacaat aaatggtgaa gaacagcaaa ataaccagcg
     3361 cggagataat gcgcaaaatg cggcttttat cttcaaagcg tccggtgaaa taatccggca
     3421 atgttaaggc gttattgtta tattcggtat gcacacgcaa ccgcccggcc accagcttcc
     3481 agttgatcca cgcgcctaat gtcaggccaa tggcgatcca actttcggaa atcccggaaa
     3541 gaaaaaccgc gcccggcaac cccattaaca gccagccgct catatccgat gcacccgccg
     3601 ataatgccgt cacgaatggc ccaaggctac gtccgcccag aatatagtcg tcaaagtttt
     3661 tcgttgatcg ccaggcgata aacccaatca atatcatgcc aaagatatag acacaaaatg
     3721 tcaccaacat cggtgtgcta atagccatct aaagtctcca aaaaattatt atcggcaatg
     3781 tcgaaacttg ccgttatatc tgccaccgga acggggtaac agagtttatg ttttaccagg
     3841 gcgaccgtat cctgccggaa gcgctagtta ttcacaatcg atttaacaca ccatttacat
     3901 taaattttag tgctcagcga cactattttt catcaggttg cactctctca cattttttgc
     3961 ggttgcacct ttcaaaaatg ttaactgccg cagagaaaaa gtctgagtta tttttttaat
     4021 ccctgtcata tcgatttctt ttattaacat ttcattcatt tttaagcttg ctacgcaagt
     4081 cacatttaac atggttgcac aaagttgcaa catcatggat atttcacgat aacgttaagt
     4141 tgcacctttc agaacaacag gagtaatggc atgggaacca ccaccatggg ggttaagctg
     4201 gacgacgcga cgcgtgagcg tattaagtct gccgcgacac gtatcgatcg cacgccacac
     4261 tggttaatta agcaggcgat tttttcttat ctcgaacaac tggaaaacag cgatactctg
     4321 ccagagctac ctgcgctgct ttctggcgct gccaatgaga gcgatgaagc accgactccg
     4381 gcagaggaac cacaccagcc attcctcgac tttgccgagc aaattttgcc ccagtcggtt
     4441 tcccgcgccg cgatcaccgc ggcctatcgc cgcccggaaa ccgaagcggt ttctatgctg
     4501 ctggaacaag cccgcctgcc gcagccagtt gctgaacagg cacacaaact ggcgtatcaa
     4561 ctggccgata aactgcgtaa tcaaaaaaat gccagtggcc gcgcaggtat ggtccagggg
     4621 ttattgcagg agttttcgct gtcatcgcag gaaggcgtgg cgctgatgtg tctggcagaa
     4681 gcgttgttgc gtattcccga caaagccacc cgcgacgcgt taattcgcga caaaatcagc
     4741 aacggtaact ggcagtcaca cattggtcgc agcccgtccc tgtttgttaa tgccgccacc
     4801 tgggggttgt tgtttactgg caaactggtt tccacccata acgaagccag cctctcccgc
     4861 tcgctgaacc gcattatcgg taaaagtggt gaaccgctga tccgcaaagg tgtggatatg
     4921 gcgatgcgcc tgatgggcga gcagttcgtc acgggcgaaa ccatcgcgga agcgttagcc
     4981 aatgcccgca agctggaaga gaaaggtttc cgttactctt acgatatgct tggcgaagct
     5041 gcactaactg ccgcagatgc acaagcgtat atggtttcct atcagcaggc gattcacgcc
     5101 attggtaaag cgtctaacgg tcgtggcatc tatgaaggtc cgggcatttc aatcaagctg
     5161 tcggcgctgc atccgcgtta cagccgcgcc cagtatgacc gggtaatgga agagctttac
     5221 ccacgtctga aatcactcac cctgctggcg cgtcagtacg atattggtat caacattgac
     5281 gccgaagagg ccgatcgtct ggagatctcc ctcgatctgc tggaaaaact ctgtttcgag
     5341 ccggaactgg caggctggaa cggcatcggc tttgttattc aggcttatca aaaacgctgc
     5401 ccactggtga ttgattacct gattgatctc gccacccgca gccgtcgccg tctgatgatt
     5461 cgcctggtaa aaggcgcgta ctgggatagt gaaatcaagc gtgcacagat ggacggcctt
     5521 gaaggttatc cggtttatac ccgcaaggtg tataccgacg tttcttatct cgcctgtgcg
     5581 aaaaagctgc tggcggtgcc gaatctaatc tacccgcagt tcgcgacgca caacgcccat
     5641 acgctggcgg cgatttatca actggcaggt caaaactact atccgggtca gtacgagttc
     5701 cagtgcctgc atggtatggg cgagccactg tatgagcagg tcaccggaaa agttgccgac
     5761 ggcaaactta accgtccgtg tcgtatttat gccccggttg gcacacatga aacactgctg
     5821 gcgtatctgg tgcgtcgcct gctggaaaac ggtgctaaca cctcgtttgt taaccgtatt
     5881 gccgacacct ctttgccatt ggatgaactg gtcgccgatc cggtcactgc tgtagaaaaa
     5941 ctggcgcaac aggaagggca aactggatta ccgcatccga aaattcccct gccgcgcgat
     6001 ctttatggtc acgggcgcga caactcggca gggctggatc tcgctaacga acaccgtctg
     6061 gcctcgctct cttctgccct gctcaatagt gcactgcaaa aatggcaggc cttgccaatg
     6121 ctggaacaaa cggtagcggc tggtgagatg tcgcccgtta ttaaccctgc ggaaccgaaa
     6181 gatattgtgg gctatgtgcg tgaagccacg ccgcgtgaag tagaactggc gctggaaagt
     6241 gcggttaata acgcgccaat ctggtttgcc acgcctccgg ctgaacgcgc ggcgattttg
     6301 catcgcgctg ccgtgctgat ggaaagccag atgcagcaac tgattggtat tctggtgcgt
     6361 gaggccggaa aaaccttcag taacgccatt gccgaagtgc gtgaagcggt cgattttctc
     6421 cactactacg ccggacaggt gcgggatgat ttcgctaacg aaacccatcg tccattaggg
     6481 cctgtggtat gtatcagccc gtggaacttc ccgctggcga ttttcaccgg gcagattgct
     6541 gccgcactgg cggcaggtaa cagcgtgctg gcaaaaccgg cagaacaaac gccgctgatt
     6601 gccgcgcaag ggatcgccat tttgctggaa gcgggtgtac cgccaggcgt ggtgcagttg
     6661 ctgccaggtc agggggaaac cgtgggcgcg caactgacgg gtgatgatcg cgtgcgcggg
     6721 gtgatgttta ccggttcaac cgaagtcgct acgttactgc agcgcaatat cgccagccgc
     6781 ctggacgctc agggtcgccc tattccgctc atcgctgaaa ccggcggcat gaacgcgatg
     6841 attgtcgatt cttcagcact gaccgaacaa gtagtaatag atgtgctggc ctcggcgttc
     6901 gacagtgcgg gtcagcgttg ttcggcactg cgcgtgctgt gcctgcaaga tgagattgcc
     6961 gaccacacgt tgaaaatgct gcgcggcgca atggccgaat gtcggatggg taatccgggt
     7021 cgcctgacca ctgatatcgg tccggtgatt gatagcgaag cgaaagccaa tatcgagcgt
     7081 catattcaga ccatgcgtag caaaggccgt ccggtgttcc aggcggtacg ggaaaacaac
     7141 gaagatgccc gtgaatggca aagcggcacc tttgtcgccc cgacgctgat cgaactggat
     7201 gactttgccg aattgcaaaa agaggtcttt ggtccggtgc tgcatgtggt gcgttacaac
     7261 cgtaaccagc tgccggagct gatcgagcag attaacgctt ccggttatgg tctgacgctt
     7321 ggcgtccaca cgcgcattga tgaaaccatc gcccaggtca ctggctcggc ccatgtcggt
     7381 aacctgtacg ttaaccgtaa tatggtgggc gcagtggtgg gtgtgcagcc attcggcggc
     7441 gaaggattgt ccggcaccgg gccaaaagca ggcggtccgc tctatctcta ccgtctactg
     7501 gcgaatcgcc cggaaagtgc gctggcagtg acgctcgcgc gtcaggatgc agagtatccg
     7561 gtcgatgcgc agttgaaagc cgcattgact cagccgctaa atgcactgcg ggaatgggca
     7621 gcaaatcgcc cagaattgca ggcgttatgt acgcaatatg gcgagctggc gcaggcagga
     7681 acacaacgat tgctgccagg gccgacgggt gagcgcaaca cctggacgct gctgccgcgt
     7741 gagcgcgtat tgtgtatcgc ggatgatgaa caggatgcgc tgactcagct cgccgccgtg
     7801 ctggcggtgg gcagccaggt gctgtggcca gatgacgcgc tgcatcgtca gttagtgaag
     7861 gcattgccat cgactgtcag cgagcgtatt caactggcga aagcggaaaa tattaccgct
     7921 caaccgtttg atgcggtgat cttccacggt gattcggatc agcttcgcgc attgtgtgaa
     7981 gcagttgccg cacgggatgg cgcaattgtt tcggtgcagg gttttgcccg tggcgaaagc
     8041 aatatccttc tggaacggct gtatatcgag cgttcgctga gtgtgaatac cgctgccgct
     8101 ggcggtaacg ccagcttaat gacgatcggt taaacaggta gccggaggat gttacacctt
     8161 cctccggcat cattagcgtg gtcgaatccc ctcaataata atccgctgca cgttttcaac
     8221 cgtttgattg aaaaatacct catcgcgcaa cgttgcgcct gtcaccgcct ccacctgagg
     8281 ggcgaaatcg gcgtaatgtt gagtggaagc ccaaatcata aaaatcaaat gctgcggatc
     8341 aatcggcgcg agtttgccgc ttttgaccca accggcaatc agcgccgatt tctcatcaat
     8401 taatgccttc aaatcgcccg tcagttcatc cattaacagc ggcgcgcctg ccagcatctc
     8461 catacagaac aggcgcgaag cctgcggata atcgcgtgag acttccagct tcagacggat
     8521 gtactctttg atcgccgcca gcggggcgaa atcttcacga aacgctttta acggtgccag
     8581 ccagatatcg agaatctgcc gcagcacggc aatatacagc gcctcttttg acggaaagta
     8641 atacagcaga ttggtttttg aaacacccgc caactctgcg atctgctcca gccttgtgcc
     8701 gtgaaaaccg aattgtgaaa aagtgtccag tgctgcgcta agaatcgctt ttttcttcgc
     8761 actgactgcg cgcgaacgtt tacccgttgt tttcactgca ccttgcgtca tgcgctctcc
     8821 cctcttgtag attggcaaca ggatagcaaa acccccgccc ggcctcgacc ttctgcactc
     8881 tcatcgcgca cggatgcatg ttttatgtgc aactgttttg accgtttagt ccacttttta
     8941 ccagatattt tagataacgc aatgttaaca tattaataac attcactttt taaaaactgg
     9001 catccacttt gcaaacaagc caatacgcgg ctgaaaagga tatgcaggat gcagcgcccc
     9061 gtctgacttt cactttacga gacgaagaga ggttgatgat gaaaattggc gtattcgtac
     9121 ctattggcaa caacggctgg ctcatttcga cccacgcgcc gcagtacatg ccgacctttg
     9181 aactgaataa agccatcgtg caaaaagcgg agcactacca ttttgatttc gccctgtcga
     9241 tgatcaaact gcgtggcttt ggcggcaaaa ctgagttctg ggatcacaac cttgagtcgt
     9301 tcaccttgat ggcggggctg gcagccgtga cctcgcgcat tcagatttac gccaccgccg
     9361 ccaccttaac gttacctccg gcaatcgtcg cccgtatggc cgcaaccatc gactccatct
     9421 ctggcgggcg ttttggcgtc aacctcgtga ctggctggca aaagcccgag tatgagcaga
     9481 tgggtatctg gcctggcgat gactatttct cccgtcgtta cgactatctc accgaatatg
     9541 ttcaggtgtt gcgcgacctg tggggcacgg gaaaaagcga ttttaaaggc tattttttca
     9601 ccatgaatga ttgtcgcgtc agtccgcaac cgagtgtccc catgaaagtg atctgcgccg
     9661 ggcaaagcga cgctggcatg gcgttctccg ctcagtatgc cgatttcaac ttctgtttcg
     9721 gcaaaggcgt aaatacacct acggctttcg ccccgaccgc tgtgcggatg aaacaggccg
     9781 cagagcaaac cgggcgcgac gttggctctt atgtgttgtt tatggtgatt gccgatgaaa
     9841 ccgacgatgc cgctcgtgcc aaatgggaac actacaaagc gggcgcggat gaagaggcgt
     9901 taagctggct aaccgaacaa agtcagaaag atacccgctc cggtactgac accaacgtcc
     9961 gtcagatggc agatcccact tcggcggtaa acatcaatat ggggacgtta gtcggttctt
    10021 acgccagtgt cgcgcgcatg ttagatgaag tcgcaagcgt gcctggtgcc gaaggcgtgc
    10081 tgttaacctt cgacgatttt ctgtcgggaa tcgaaacctt cggcgagcgc attcaaccac
    10141 tgatgcagtg ccgcgcccat ctccctgtgc tgactcagga ggtggcatga tgacgacatt
    10201 aaccgctcga ccggaagcca ttaccttcga tccgcagcaa agtgcgctga tcgtggtgga
    10261 tatgcaaaac gcctatgcca cgccaggcgg ctacttagat ctcgccgggt ttgatgtctc
    10321 aaccactcgc ccggtcattg ccaacattca aaccgccgtg accgcagcgc gagcggcagg
    10381 gatgttgatc atctggtttc aaaatggctg ggatgaacag tatgtcgagg ctggcggccc
    10441 cggctcaccg aattttcata aatcgaacgc cttgaaaacc atgcgtaagc agccgcagct
    10501 acaggggaaa ttgctggcga aaggctcctg ggattatcaa ctggtggatg aactggtgcc
    10561 gcagcctggc gatattgtgc tgccgaagcc gcgctacagc ggtttcttca atacgccgct
    10621 ggacagcatt ttgcgcagcc gcggaatacg ccatctggtt ttcaccggca tcgctaccaa
    10681 cgtctgcgtc gaatcgacgc tacgcgacgg cttttttctg gagtatttcg gcgtggtgct
    10741 ggaagacgca acacaccagg cggggccgga atttgtacag aaagccgcgt tgttcaatat
    10801 cgaaaccttt tttggctggg tcagcgacgt cgaaacgttc tgcgacgcgc tttctcccac
    10861 gtcctttgct cgtatcgctt aaggagttta acgatgccaa aatccgtaat tattcccgct
    10921 ggcagcagcg caccgctggc ccccttcgtt cccggcacgc tggctgatgg cgtggtgtat
    10981 gtctccggta cgctggcttt tgatcaacac aataacgtgc tgtttgccga tgacccaaag
    11041 gcgcaaaccc gccacgttct ggaaactatc cgcaaggtga tcgagacagc gggtggcacg
    11101 atggcggatg tgaccttcaa cagcatcttt attaccgact ggaaaaatta cgccgcgatt
    11161 aacgaaatct acgccgagtt ttttccaggt gataaaccgg cgcgattctg cattcagtgc
    11221 ggactggtaa aacctgacgc gctggtggaa atcgccacaa ttgcgcatat cgccaagtga
    11281 ggccgcgatg aaactttcac tctcacctcc cccttatgct gatgcacccg tagtggtgtt
    11341 gatttcgggt cttggcggta gcggcagtta ctggttaccg caactggcgg tgctggagca
    11401 ggagtatcag gtagtctgtt acgaccagcg cggcaccggc aataatcccg acacactggc
    11461 agaagattac agtatcgccc agatggcagc ggaactgcat caggcgctgg tagccgcagg
    11521 gattgagcat tacgcggtgg tcggccatgc gctcggtgcg ctggtgggaa tgcagctggc
    11581 gctggattat cccgcgtcgg taactgtgct ggtcagcgtt aacggctggc tacgaataaa
    11641 cgcccatacg cgccgctgtt ttcaggttcg cgaacgatta ctgtatagcg gcggcgcgca
    11701 ggcatgggtg gaagcgcagc cgttgttcct ctatcccgcc gactggatgg cggcccgcgc
    11761 acctcgcctg gaggcagaag acgcgctggc actggcgcat tttcagggca aaaataattt
    11821 actgcgtcga cttaacgccc tcaaacgcgc tgactttagt caccatgcgg atcgcatccg
    11881 ctgcccggtg caaatcatct gcgccagtga tgatctgctg gtgccatcag catgttccag
    11941 tgaacttcat gccgccctgc ccgatagcca gaaaatggtg atgcgctatg gcggacacgc
    12001 ctgcaacgtg accgatcccg aaacgtttaa tgctctgtta ctcaacgggc ttgccagcct
    12061 gttacatcac cgtgaagccg ccctgtaagg aattgctatg aacgaagccg ttagcccagg
    12121 tgcgcttagc accctgttca ccaatgcccg cactcacaat ggctggcggg agacacccgt
    12181 cagcgatgag acgttacggg agctttatgc cctgatgaaa tgggggccga catcagctaa
    12241 ctgttctccg gcacgtatcg tgtttatccg cactgcagag ggaaaagaac gtctgcgccc
    12301 ggcgctttcc agcggcaatt tgcaaaaaac cctgaccgcg cccgtcaccg ctatcgtcgc
    12361 atgggacagt gaattttatg aacggttacc gcaactgttt cctcacggcg atgcccgcag
    12421 ttggtttacc tccagcccac aacttgccga agaaacagcg tttcgcaaca gttccatgca
    12481 ggcggcctat ctgatcgtcg cctgccgtgc gctggggctg gatactggcc cgatgtcggg
    12541 ttttgaccgt caacacgtgg acgacgcctt ttttgcgggt agcacgctga agagcaatct
    12601 gctgattaat atcggctatg gcgatagcag caaacttttt gcgcgcctgc cacgtctgtc
    12661 ctttgaagaa gcctgcgggc tgttgtaagg agcaaactat gaacattgtc gatcaacaaa
    12721 cttttcgcga tgcgatgtcc tgcatgggtg cggcggtcaa tatcatcacc acggacggtc
    12781 cagccgggcg cgccgggttc accgccagcg ccgtctgcag cgtgaccgat acgcctccca
    12841 cattactggt gtgcctgaat cgtggggcgt ccgtctggcc ggtattcaat gaaaatcgaa
    12901 cgctgtgtgt aaatacactt agcgccgggc aggagccgct ttcaaacctt tttggcggca
    12961 aaacgcccat ggaacaccgc tttgccgccg cccgctggca gactggtgtg accggatgcc
    13021 cgcaactgga agaggcgctg gtttcgtttg actgccgtat cagccaggtg gtgagcgtcg
    13081 gcacccacga cattttgttt tgcgccatcg aagcgattca tcgtcacacc acaccctacg
    13141 ggctggtgtg gttcgatcgc agttatcacg cgctgatgcg ccctgcttgt taacctcctt
    13201 aaggagacag ctgatggcaa tgttcggtct tcctcactgg cagttaaaat cgacctctac
    13261 agagagcggc gtggtcgcgc cggatgaacg attgccgttt gctcagacgg caataatggg
    13321 cgttcaacac gcggtggcga tgtttggcgc gacggtgtta atgccgattc tgatggggct
    13381 ggatcccaat ctttccattt taatgtcagg gatcggcacg ttactgtttt tctttattac
    13441 cggcgggcgc gtgcccagtt atctcggctc cagcgcggct tttgttggtg tggtgatcgc
    13501 ggcaacgggc tttaacggcc aggggattaa cccgaatatc agcatcgcgc tgggcgggat
    13561 tatcgcctgt gggctggttt ataccgtcat tggcctggtg gtgatgaaaa tcggtacgcg
    13621 ctggattgaa cggctaatgc cgccggtggt gacgggtgcg gtggtgatgg cgattggcct
    13681 gaacttagcg cctatcgcgg taaaaagtgt atcggcttca ggctttgata gctggatggc
    13741 ggtaatgacg gtgctgtgta ttgggctggt ggcggtgttt acccgtggca tgatccagcg
    13801 gttattgatt cttgtaggat taattgtcgc ctgtctgctg tatggcgtga tgaccaacgt
    13861 tttagggctg ggaaaagccg tcgattttac tctcgtcagc catgctgcct ggttcggtct
    13921 accgcatttt tccactcctg gttttaatgg tcaggcaatg atgctgattg cgcccgtggc
    13981 ggtgattctg gtggcggaaa acttaggcca tctcaaggcg gtcgccggga tgaccggacg
    14041 caatatggat ccgtatatgg gacgggcatt cgtcggtgat gggctggcga cgatgctttc
    14101 tggttctgtc ggtggcagcg gcgtcaccac ctatgccgag aatatcgggg taatggccgt
    14161 gacaaaagtc tactcgacgc tggtgtttgt cgctgctgcc gtcatcgcca tgctattggg
    14221 cttttcaccg aaatttggcg cgctgattca taccattccg gccgcggtga ttggtggtgc
    14281 atcaattgtt gtgttcgggc tgattgccgt cgcaggcgca aggatatggg tacaaaaccg
    14341 tgtcgattta agccagaacg gtaatttgat tatggtcgca gtaaccttag tgctgggcgc
    14401 gggtgatttt gctctcacgc tgggcggttt tacgttggga gggatcggta cagcaacctt
    14461 tggcgcgatt ttactcaatg cgttattaag cagaaggttg gttgacgttc caccgccaga
    14521 agttgttcat caggagccat aaacagaaaa ccgccgggat taccggcggt gatgactttt
    14581 tgacgagctt aagaaaaaaa tgcattcgtc attgattagc ccggctagtt gtcgcttttg
    14641 ccgtgactgc tcttgccacc ttttttacct gcttcagatg cgcgctgcgg atcattcttg
    14701 aaattacccc cgctgtgctg tccacctttt ttacctgctt ctgatgctct ttcgcggtct
    14761 tctgcaaaat tgccggaacc gcctcgatgg tttgccatta ctgacctccg tctgggtata
    14821 gacatcatat tgcatagaat taacgcggcg tatggcggct gccatatttt cgctacgtgg
    14881 gactaagctt agtgaactga cgtgtttcta cccgcgtttg gtaatatcct gcaacagctt
    14941 cttagcgaga atagatatca tctccaataa ttatccataa gccgctctta tgattaaaaa
    15001 aacgcgatga gataaaaatg tgtcattttg cgacaaaatt gcgtgcttgt gaaagttgtt
    15061 ataaatcaaa taagtggttg tgaaatttgc actctgaaaa ggacgtctta tctttaaata
    15121 agtggtagcg aatcgctacg gaatagagat aacacgagga gtggttagaa atggctaaag
    15181 ttctggtgct ttattattcc atgtacggac atattgaaac gatggcacgc gcagtcgctg
    15241 agggtgcaag caaagtggat ggcgctgaag ttgtcgttaa gcgtgtaccg gaaaccatgc
    15301 cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac tgcaccggtt gcaaccccgc
    15361 aagaactggc cgattacgac gccattattt ttggtacacc tacccgcttt ggcaacatgt
    15421 ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct gtgggcttcc ggcgcactat
    15481 acggaaaact ggcgagcgtc tttagttcca ccggtactgg cggcggtcag gaacaaacca
    15541 tcacatccac ctggacgacc cttgcgcatc acggcatggt gattgtcccc attggctacg
    15601 cagcgcagga attatttgac gtttcacagg ttcgcggcgg tacgccgtac ggcgcaacca
    15661 ccatcgcagg cggtgacggt tcacgccagc ccagccagga agaactgtct attgctcgtt
    15721 atcaagggga atatgtcgca ggtctggcag ttaaacttaa tggctaatct tcaacaggag
    15781 gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc ggtgaatggg catctctgcg
    15841 caatacgtcg ccggaaatag ctgaggccat tttcgaagtc gccgggtacg acgaaaaaat
    15901 ggcggaaaag atttgggaag aaggtagtga tgaagtgtta gtcaaagcct ttgctaagac
    15961 cgataaagat tcgctttttt ggggcgaaca gaccatagaa cgtaaaaacg tttaaccagc
    16021 gactcccccg tttctcacgg gggagttttc tgttatttca ccgcttcatt caacacgcta
    16081 tcaaacttat ccatcgggca gaaaccgttg gcatctatcg ggcaaccgct taattccagc
    16141 gtcacacgct gcgcaggtgc ctgcagggtt aacgcatcgg cattacgtaa ctgttccgca
    16201 ctctggtaca catactcgat tttcatcaaa tcgcgattgg ctttgctgtc atgccaacgc
    16261 tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct ggtcatgcaa ctgatacggt
    16321 ttgaaatcca gcgccgttaa cagagaggca atgttggagt catgcccaac taacaccgtg
    16381 atttttggtg cactggtgcg atcggtgacc agggctttgt cgatataact gaccagcggt
    16441 ttcgcaacat tgcgcgccac ttccggtgag gtaaacaggc tgtcctggta accgtttttc
    16501 agcttcgaca acaccttcca ctgctggtca gatttgattt ctccccaggc cacctgatcc
    16561 atcggaaaac cttcgtagta ttgcaaagta aacgcatcta ccagcgagtt gccgactttc
    16621 agcggcccgg aaacacctgg ttcttgttga tacttcgcgc taaaggtatt tttgccatcc
    16681 accagcgaac actgctgttt ctctttacag gcaggggaat ctttatagtt aacgattttt
    16741 tccagtagct ggtagctgtc ggtaagctgg agtttgctga gctctttctc cattgccgcc
    16801 accgcctgtt cactgaatgc ggcggaatca tcggtgatca ccgggttaaa ggttgggtcc
    16861 atggtgccca ttttttcctg gtgatgcaca ggaatatcac accccgggaa tgcgccggta
    16921 ataaagaact gtgcggtcgc gacagtacgt tgcagactat tggcataggc ataaacggtg
    16981 tccggaggcg ggcattcccc cgatttcacc atcccctgct ctgccagcca ttcacgcatg
    17041 taatggccca tatacacttc gagcacgcca cctttggtgg tgagttgccc accggggacg
    17101 tcccattctg gccatttatt cggcgtcgac tgctccagca cactgccatt gttcgccagc
    17161 ggcgcacgta agttatggcg gctcatcatg agcacttgct gtagctgata gccttccggt
    17221 acggtttgtg cctgagcatt tgaagcgagt aaaactatcc ctgccacagt tgcggtgatt
    17281 agcgttttgt tcattcctgg cacctctttt gttatcaatc actaaagagt gtgacagaaa
    17341 tatgactttt ttccgggaac aggttcgcaa aatggcttgt tgaacgcctg tttttctata
    17401 tacatcttca aaaacaggca aggtttgacg caaactatgc ctgattagcc cctgcctcgc
    17461 gtacgccgct gataattgca cctgtggcta aacagaatat ctgcggcgtt accaatacag
    17521 agacaatatt taacaataaa aaagtgtgag actaataaca atggttttta gcatatgcct
    17581 ttgccattaa attgcataac gatttaaata gtcaagttgc gcattcattt ccttattgtt
    17641 taacttgtaa gcctgagcaa aaagttcagg agggaggtaa acgtcataat gattgccggt
    17701 gtttattaaa taaccatact caagatcttc atctgttgaa aaggattcat tccccaagga
    17761 gattgatgcc acattactat taacaataaa taatgagcaa atgctatctg aaagagcagt
    17821 gaggaaattt ttcacctcat taat
//
LOCUS       DAZFKF010000102        17787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFKF010000102 DAZFKF010000000
VERSION     DAZFKF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.102"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            329..544
                     /gene="essD"
                     /locus_tag="ACHKA1_004096"
     CDS             329..544
                     /gene="essD"
                     /locus_tag="ACHKA1_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO5681669.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            544..1041
                     /gene="rrrD"
                     /locus_tag="ACHKA1_004097"
     CDS             544..1041
                     /gene="rrrD"
                     /locus_tag="ACHKA1_004097"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO5681670.1"
                     /translation="MPPSLRKVVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            1258..1440
                     /gene="rzoD"
                     /locus_tag="ACHKA1_004098"
     CDS             1258..1440
                     /gene="rzoD"
                     /locus_tag="ACHKA1_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO5681671.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1531..1824)
                     /gene="iss"
                     /locus_tag="ACHKA1_004099"
     CDS             complement(1531..1824)
                     /gene="iss"
                     /locus_tag="ACHKA1_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO5681672.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(2184..2378)
                     /locus_tag="ACHKA1_004100"
     CDS             complement(2184..2378)
                     /locus_tag="ACHKA1_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5681673.1"
                     /translation="MSTKNKTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDE"
     gene            2767..3312
                     /locus_tag="ACHKA1_004101"
     CDS             2767..3312
                     /locus_tag="ACHKA1_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO5681674.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            3287..5212
                     /locus_tag="ACHKA1_004102"
     CDS             3287..5212
                     /locus_tag="ACHKA1_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5681675.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            5209..5415
                     /locus_tag="ACHKA1_004103"
     CDS             5209..5415
                     /locus_tag="ACHKA1_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO5681676.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            5412..7013
                     /locus_tag="ACHKA1_004104"
     CDS             5412..7013
                     /locus_tag="ACHKA1_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5681677.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            6994..8313
                     /locus_tag="ACHKA1_004105"
     CDS             6994..8313
                     /locus_tag="ACHKA1_004105"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO5681678.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLAAGEAPATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGIMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATALQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            8323..8655
                     /locus_tag="ACHKA1_004106"
     CDS             8323..8655
                     /locus_tag="ACHKA1_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO5681679.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            8710..9735
                     /locus_tag="ACHKA1_004107"
     CDS             8710..9735
                     /locus_tag="ACHKA1_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5681680.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     THARGLRTYGCIQDADALSEGINASPRYPKNWKTSGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            9777..10172
                     /locus_tag="ACHKA1_004108"
     CDS             9777..10172
                     /locus_tag="ACHKA1_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO5681681.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            10184..10537
                     /locus_tag="ACHKA1_004109"
     CDS             10184..10537
                     /locus_tag="ACHKA1_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO5681682.1"
                     /translation="MADFDNQFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            10549..11127
                     /locus_tag="ACHKA1_004110"
     CDS             10549..11127
                     /locus_tag="ACHKA1_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5681683.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARIVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            11124..11519
                     /locus_tag="ACHKA1_004111"
     CDS             11124..11519
                     /locus_tag="ACHKA1_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO5681684.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            11527..12267
                     /locus_tag="ACHKA1_004112"
     CDS             11527..12267
                     /locus_tag="ACHKA1_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5681685.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            12283..12705
                     /locus_tag="ACHKA1_004113"
     CDS             12283..12705
                     /locus_tag="ACHKA1_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5681686.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            12687..13121
                     /locus_tag="ACHKA1_004114"
     CDS             12687..13121
                     /locus_tag="ACHKA1_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5681687.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            13114..15675
                     /locus_tag="ACHKA1_004115"
     CDS             13114..15675
                     /locus_tag="ACHKA1_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5681688.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSVSGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAETAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHADLLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMTGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSLADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            15672..16001
                     /locus_tag="ACHKA1_004116"
     CDS             15672..16001
                     /locus_tag="ACHKA1_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5681689.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            16001..16699
                     /locus_tag="ACHKA1_004117"
     CDS             16001..16699
                     /locus_tag="ACHKA1_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5681690.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            16704..17447
                     /locus_tag="ACHKA1_004118"
     CDS             16704..17447
                     /locus_tag="ACHKA1_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5681691.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAKMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGVIHK
                     FRCVPHLTGRRFEHGATDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNMEAT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLAAASTFV"
     gene            17444..>17787
                     /locus_tag="ACHKA1_004119"
     CDS             17444..>17787
                     /locus_tag="ACHKA1_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5681692.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLCDGWYQVRIAGRDVSTSGLTVQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVL"
BASE COUNT         4098 a         4434 c         5410 g         3845 t
ORIGIN      
        1 ctaaaaataa cgtcggaaat tatttattgg ttatttgttg aggttttctt atgtatttgt
       61 ggtggtgttt tgaacactcg gtagcattct cataaatatc attcagtggt ttacgtacgt
      121 aaaaaattgg ttatgctgtt aagagtggtt acttcgtcac acagcttaaa cccgccgtcg
      181 agctggtttt tccatttttt gagtctcgat attagctgat aactcaatac ctaagttatt
      241 cactgactcc gagtctgtta cgtttctgct tttttgcgat acgttgtatt ccctcaattt
      301 acacccgctt tgtctgcgag gtggggttat gaaatccatg gataagttaa caacgggtgt
      361 cgcctatggc acctcagcag gtagtgccgg ttactggttt ttacagctgc tcgataaagt
      421 cacgccctca cagtgggcag caataggtgt gctgggtagc ctggtatttg gcctgctgac
      481 gtacctgaca aacctttatt tcaagattaa agaagataag cgcaaggctg cgagaggtga
      541 ataatgcctc catcattacg aaaagtcgtt gctgctgcta ttggtggcgg agcaattgct
      601 atagcatcag tgttaatcac tggcccaagt ggtaacgatg gtctggaagg tgtcagctac
      661 ataccataca aagatattgt tggtgtatgg actgtatgtc acgggcatac aggaaaagac
      721 atcatgctcg gtaaaacgta taccaaagca gaatgcaaag cactcttgaa taaagacctt
      781 gccactgtcg ccagacaaat taacccgtac atcaaagtcg atataccgga aacaacgcgc
      841 ggcgctcttt actcattcgt ttacaacgtg ggtgctggca atttcagaac atcgacgctt
      901 cttcgcaaaa taaaccaggg cgatatcaaa ggcgcatgtg atcagctacg tcgctggaca
      961 tatgctggcg gtaagcaatg gaaaggtctc atgactcgtc gtgagattga gcgtgaaatc
     1021 tgtttgtggg gtcagcaatg aacagagtaa ccgcgattat ctccgctctg gttatctgca
     1081 tcatcgtctg cctgtcatgg gctgttaatc attaccgtga taacgccatt acctacaaag
     1141 cccagcgcga caaaaatgcc agagaactga agctggcgaa cgcggcaatt actgacatgc
     1201 agatgcgtca gcgtgatgtt gctgcgctcg atgcaaaata cacgaaggag ttagctgatg
     1261 cgaaagctga aaatgatgct ctgcgtgatg atgttgccgc tggtcgtcgt cggttgcaca
     1321 tcaaagcagt ctgtcagtca gtgcgtgaag ccaccaccgc ctccggcgtg gataatgcag
     1381 cctccccccg actggcagac accgctgaac gggattattt caccctcaga gagaggctga
     1441 tcactatgca aaaacaactg gaaggaaccc agaagtatat taatgagcag tgcagataga
     1501 gctgcccata tcgatgggca actcatgcaa ttattgtgag caatacacac gcgcttccag
     1561 cggagtataa atgcctaaag taataaaacc gagcaatcca tttacgaatg tttgctgggt
     1621 ttctgtttta acaacatttt ctgcgccgcc acaaattttg gctgcatcaa cagttttctc
     1681 ctgtccaatt cccgaaacga agaagtgatg ggtgatggtt tcctttggtg ttactgctgt
     1741 cggtttgttt ccaacagtaa acgtctgttg agcacatcct gtaataagca ttgccagagc
     1801 ggcagaaaac aacatttttt tcatcttatt atcctgcatt gttaaaaacg gcagaatcct
     1861 atgtgacaac aattaaacga tagttaaatg gattgatgaa aattaaaact atataggtgg
     1921 atgctcagcc tattggagga ggggggcact cagaatcctg tggaatgaaa taaaccgctc
     1981 tatctgtcca ttaccctttt agctgcgctg tatcgtcgcc gtattcccgc attaaccatg
     2041 accgtagccc gacggggaat tccttctgcg tgagtgtgcg ggaataatca aaaacgatgc
     2101 acaccgggtt ttactgtgct gacagacgca gggttaccct catagtcgct tttccggtgc
     2161 gatggtggaa gaaaccggga tgtttattca tcatcacttt ggattgatgt atatgctctc
     2221 ttttctgacg ttagtctccg acggcaggct tcaatgaccc aggctgagaa attcccagac
     2281 cctttttgct caagagcgat gttaatttgt tcaatcattt ggttaggaaa gcggatgttg
     2341 cgggttgttg ttctgcgggt tttgttcttc gttgacatga ggttgccccg tattcagtgt
     2401 cgctgatttg tattgtctga agttgttttt acgttaagtt gatgcagatc aattaatacg
     2461 atacctgcgt cataattgat tatttgacgt ggtttgatgg cgtagatgca cgttgtgaca
     2521 tatagatgat aattattatc attttgcggg tcctttccgg cgatccgaca ggttacgggg
     2581 cggcgacctc gcgggttttc gctatttatg aaaattttcc ggtttaaggc gtttccgttc
     2641 ttctttgccg taacttaatg tttttattta aaacaccctc tgaaaagaaa ggaaacgaca
     2701 ggtgctgaaa acgagctttt tggcctctgt cgtttccttt ctctgttttt gtccgtggaa
     2761 tgaacaatgg aagtcaacaa aaagcagctg gctgacattt tcggcgcgag tatccgtacc
     2821 attcagaact ggcaggagca gggaatgccc gttctgcggg gtggtggcaa gggtaatgaa
     2881 gtgctttatg actctgccgc cgccataaaa tggtatgccg aaagggatgc tgaaattgag
     2941 aacgaaaagc tgcgccggga agttgaagaa ctgcggcagg ccagcgagac agatctccag
     3001 ccagggacta ttgagtacga acgccatcga cttacgcgtg cgcaggccga cgcacaggag
     3061 ctgaaaaatg ccagagactc cgctgaagtg gtggaaaccg cattctgtac tttcgtgctg
     3121 tcgcggatcg caggtgaaat tgccagtatt ctcgacggga tccccctgtc ggtgcagcgg
     3181 cgttttccgg aactggaaaa ccgacatgtt gatttcctga aacgggatat catcaaagcc
     3241 atgaacaaag cagccgcgct ggatgaactg ataccggggt tgctgagtga atatatcgaa
     3301 cagtcaggtt aacaggctgc ggcattttgt ccgcgccggg cttcgctcac tgttcaggcc
     3361 ggagccacag accgccgttg aatgggcgga tgccaattac tatctcccaa aagaatccgc
     3421 ataccaggaa gggcgctggg aaacactgcc ctttcagcgg gccatcatga atgcgatggg
     3481 cagcgactac atccgtgagg tgaatgtggt gaagtctgcc cgtgtcggtt attccaaaat
     3541 gctgctgggt gtttatgcct actttataga gcataagcag cgcaacaccc ttatctggtt
     3601 gccgacggat ggtgatgccg agaactttat gaaaacccac gttgagccga ccatccgcga
     3661 tattccgtcg ctgctggcgc tggctccgtg gtatggcaaa aagcaccggg ataacacgct
     3721 cactatgaag cgtttttcca atggtcgtgg cttctggtgc ctgggcggta aagcggcaaa
     3781 aaactaccgt gaaaagtcgg tggatgtggc gggttatgat gaacttgctg cctttgatga
     3841 ggatattgaa caggaaggct ctccgacgtt cctgggtgac aagcgtattg aaggctcggt
     3901 ctggccaaag tccatccgtg gctccacgcc caaagtgaga ggcacctgtc agattgagcg
     3961 tgcagccagt gaatccccgc attttatgcg ttttcatgtt gcctgcccgc attgcgggga
     4021 ggagcagtat cttaaatttg gcgacaaaga gacgccgttt ggcctcaaat ggacgccgga
     4081 tgacccctcc agcgtgtttt atctctgcga gcataatgcc tgcgtcatcc gccagcagga
     4141 gctggacttt actgatgccc gttatatctg cgaaaagacc gggatctgga cccgtgatgg
     4201 cattctctgg ttttcgtcat ccggtgaaga gattgagcca cctgacagtg tgacctttca
     4261 catctggaca gcgtacagcc cgttcaccac ctgggtgcag attgtcaaag actggatgaa
     4321 aacgaaaggg gatacgggaa aacgtaaaac cttcgtaaac accacgctcg gtgagacgtg
     4381 ggaggcgaaa attggcgaac gtccggatgc tgaagtgatg gcagagcgga aagagcatta
     4441 ttcagcgccc gttcctgacc gtgtggctta cctgaccgcc ggtatcgact cccagctgga
     4501 ccgctacgaa atgcgcgtat ggggatgggg gccgggtgag gaaagctggc tgattgaccg
     4561 gcagattatt atgggccgcc acgacgatga acagacgctg ctgcgtgtgg atgaggccat
     4621 caataaaacc tatacccgcc ggaatggtgc agaaatgtcg gtatcccgta tctgctggga
     4681 tactggcggg attgacccga ccattgtgta tgaacgctcg aaaaaacatg ggctgttccg
     4741 ggtgatcccc attaaagggg catccgtcta cggaaagccg gtggccagca tgccacgtaa
     4801 gcgaaacaaa aacggggttt accttaccga aatcggtacg gataccgcga aagagcagat
     4861 ttataaccgc ttcacactga cgccggaagg ggatgaaccg cttcccggtg ccgttcactt
     4921 ccccaataac ccggatattt ttgatctgac cgaagcgcag cagctgacgg ctgaagagca
     4981 ggtcgaaaaa tgggtggatg gcaggaaaaa aatactgtgg gacagcaaaa agcgacgcaa
     5041 tgaggcgctc gactgctttg tttatgcgct ggcggcgctg cgcatcagta tttcccgctg
     5101 gcagctggat ctcagtgcgc tgctggcgag cctgcaggaa gaggatggtg cagcaaccaa
     5161 caagaaaaca ctggcagatt acgcccgtgc cttatccgga gaggatgaat gacgcgacag
     5221 gaagaacttg ccgctgcccg tgcggcactg catgacctga tgacaggaaa acgggtggca
     5281 acggtacaga aagacggacg gagagtggag tttacggcca cttccgtgtc tgacctgaaa
     5341 aaatacattg cggagctgga agtgcagacc ggcatgacac agcgacgcag gggacctgca
     5401 ggattttatg tatgaaaacg cccaccattc ccacccttct tgggccggac ggcatgacat
     5461 cgctgcgcga atatgccggt tatcacggtg gtggcagcgg atttggtggg cagctgcggg
     5521 cgtggaaccc accgggtgaa agtgtggatg cagccctgtt gcccaacttt acccgtggca
     5581 atgcccgcgc agacgatctg gtacgcaata acggctatgc tgccaacgcc atccagctgc
     5641 atcaggatca tatcgtcggg tcctttttcc ggctaagtca tcgcccaagc tggcgttatc
     5701 ttggcatcgg ggaggaagaa gcccgtgcct tttcccgcga ggttgaagcg gcatggaaag
     5761 agtttgccga ggatgactgc tgctgcattg acgttgagcg aaaacgcacg tttaccatga
     5821 tgattcggga aggtgtggcc atgcacgcct ttaacggtga actgttcgtt caggccacct
     5881 gggataccag ttcgtcgcgg cttttccgga cacagttccg gatggtcagc ccgaagcgca
     5941 tcagcaaccc gaacaatacc ggcgacagcc ggaactgccg tgccggtgtg cagattaatg
     6001 acagcggtgc ggcgctggga tattacgtca gcgaggacgg gtatcctggc tggatgccgc
     6061 agaaatggac atggataccc cgtgaattac ccggcgggcg cgcctcgttc attcacgttt
     6121 ttgaacccgt ggaggacggg cagacccgcg gtgcaaatgt gttttacagc gtaatggagc
     6181 agatgaagat gctcgacacg ctgcagaaca cgcagctgca gagcgccatt gtgaaggcga
     6241 tgtatgccgc caccattgaa agtgagctgg atacgcagtc agcgatggat tttattctgg
     6301 gcgcgaacag tcaggagcag cgggaaaggc tgacgggctg gattggtgaa attgccgcgt
     6361 attacgccgc agcaccggtc cgtctgggag gcgcaaaagt gccgcacctg atgccggggg
     6421 actcactgaa cctgcagacg gctcaggata cggataacgg ctactccgtg tttgagcagt
     6481 cactgttgcg gtatatcgct gccgggctgg gtgtctcgta tgagcagctt tcccggaatt
     6541 acgcccagat gagctactcc acggcacggg ccagtgcgaa cgagtcgtgg gcgtacttta
     6601 tggggcggcg aaaattcgtc gcatcccgtc aggcgagcca gatgtttctg tgctggctgg
     6661 aagaggccat cgttcgccgc gtggtgacgt taccttcaaa agcgcgcttc agctttcagg
     6721 aagcccgcag tgcctggggg aactgcgact ggataggctc cggtcgtatg gccatcgatg
     6781 gtctgaaaga agttcaggaa gcggtgatgc tgatagaagc cggactgagc acctacgaga
     6841 aagagtgcgc gaaacgcggt gacgactatc aggaaatttt tgcccagcag gtccgtgaaa
     6901 cgatggagcg ccgcgcagcc ggtcttaaac cgcccgcctg ggcggctgcg gcatttgaat
     6961 ccgggctgcg acaatcaaca gaggaggaga agagtgacag cagagctgcg taatctcccg
     7021 catattgcca gcatggcttt taatgagccg ctgatgcttg aacccgccta tgcgcgggtt
     7081 ttcttttgtg cgcttgcagg ccagcttggg atcagccgcc tgacggatgc agtatccggc
     7141 gacagcctgg ctgccggaga ggcacccgcg acgctggcgt tatccggtga tgatgacgga
     7201 ccacgacagg cccgcagtta tcaggtcatg aacggcatcg ccgtgctgcc ggtgtccggc
     7261 acgctggtca gccggacgcg ggcgctgcag ccgtattcgg gaatgaccgg ttacaacggc
     7321 attatcgccc gtctgcaaca ggctgccagc gatccgatgg tggacggcat tctgctcgat
     7381 atggacacgc caggcggaat ggtggcggga gcatttgact gcgctgacat catcgcccgt
     7441 gtgcgtgaca taaagccggt atgggcgctg gccaacgaca tgaactgcag tgcaggtcaa
     7501 ctgcttgcca gcgccgcctc ccggcgtctg gtcacgcaga ccgcccggac aggctccatc
     7561 ggcatcatga tggctcacag taattacggt gctgccctgg agaaacaggg cgtggaaatc
     7621 acgctgattt acagcggcag ccataaggtg gatggcaacc cctacagcca tctaccggat
     7681 gatgtccggg aaacactgca gtcccggatg gatgcaaccc gccggatgtt tgcacagaag
     7741 gtgtcggcat ataccggcct gtccgtgcag gctgtgctgg ataccgaggc tgcagtgtac
     7801 agcggtcagg aggccattga tgccggactg gctgatgaac ttgttaacag caccgatgcg
     7861 atcaccgtca tgcgtgatgc actggatgca cgtaaatccc gtctctcagg agggcgaatg
     7921 accaaagaga ctcaatcaac aactgtttca gccactgctt tgcaggctga cgttactgac
     7981 gtggtgccag cgacggaggg tgaaaacgcc agcgcggcgc agccagacgt gaacgcgcag
     8041 atcaccgctg cggttgcggc agaaaacagc cgcattatgg ggatcctcaa ctgtgaggag
     8101 gctcacggac gcgaagaaca ggcccgcgtg ctggcagaaa cccccggtat gaccgtggaa
     8161 acggcccgcc gcattctggc cgcagcacca cagagtgcac aggcgcgcag tgacactgcg
     8221 ctggatcgtc tgatgcaggg ggcaccggca ccgctggctg caggtaaccc ggcatctgat
     8281 gccgttaacg atttgctgaa cacaccagtg taagggatgt ttatgacgag caaagaaacc
     8341 tttacccatt accagccgct gggcaacagt gacccggctc ataccgcaac cgcgcccggc
     8401 ggattgagtg cgaaagcgcc tgcaatgacc ccgctgatgc tggacacctc cacccgtaag
     8461 ctggttgcgt gggatggcac caccgacggt gctgccgttg gcattctggc ggttgctgct
     8521 gaccagacca gcaccacgct gacgttctac aagtccggca cgttccgtta tgaggatgtg
     8581 ctctggccgg aggctgccag cgacgagacg aaaaaacgga ccgcgtttgc cggaacggca
     8641 atcagcatcg tttaacctta cccttcatca ctaaaggccg cctgtgtggc tttttttacg
     8701 ggatttttta tgtcgatgta cacaaccgcc cagctgctgg cggcaaatga gcagaaattt
     8761 aagtttgatc cgctgtttct gcgtctcttt ttccgtgaga gctatccctt caccacggag
     8821 aaagtctatc tctcacaaat tccgggactg gtaaacatgg cgctgtacgt ttcgccgatt
     8881 gtttccggtg aggttatccg ttcccgtggc ggctccacct ctgaatttac gccgggatat
     8941 gtcaagccga agcatgaggt caatccgcag atgaccctgc gtcgcctgcc ggatgaagat
     9001 ccgcagaatc tggcggaccc ggcttaccgc cgccgtcgca tcatcatgca gaacatgcgt
     9061 gacgaagagc tggccattgc tcaggtcgaa gagatgcagg ccgtttctgc cgtgctcaag
     9121 ggcaaataca ccatgaccgg tgaagccttc gatccggttg aggtggatat gggccgcagt
     9181 gtggcgaaca acatcacgca gtccggcggc acggagtgga gcaagcgtga caagtccacg
     9241 tatgacccga ccgacgatat cgaagcctac gcgctgaacg ccagcggcgt ggtgaatatc
     9301 atcgtgtttg acccgaaagg ctgggcgctg ttccgttcct tcaaagccgt caaggagaag
     9361 ctggataccc gtcgcggctc taattccgag ctggagacag cggtaaaaga cctgggcaaa
     9421 gcggtgtcct ataaggggat gtatggcgat gtggccatcg tcgtgtattc cggacagtac
     9481 gtggaaaacg gcgtcaaaaa gaacttcctg ccggacaaca cgatggtgct ggggaacact
     9541 catgcacgcg gtctgcgcac ctatggctgt attcaggatg cggatgcatt gagtgagggt
     9601 attaatgcgt ctccccgtta tccgaaaaac tggaagacat ccggcgatcc ggcgcgagag
     9661 ttcaccatga ttcagtcagc accgctgatg ctgctggctg atcctgatga gttcgtgtcc
     9721 gttcaactgg cgtaatcatg gcccttcggg gccattgtct ctctgtggag gagtccatga
     9781 cgaaagatga actgattgcc cgtctccggt cgctgggtga gcaactgaac cgtgatgtca
     9841 gcctgacggg gacgaaagaa gaactggcgc tccgtgtggc agagctggaa gaggagcttg
     9901 atgacacgga tgacgcagcc ggtcaggaca cgtctgtcag cccggaaaat gcgctgaccg
     9961 gacatgaaaa tgaggtggta tcagcacaga cggataccgt gactgatacg gctgctctgg
    10021 tcacggttgt ggcactggtg acgctgcata ccgatgcact tcacgccacg cgggatgaac
    10081 ctgtggcatt tgtgctgccg ggaacggcgt tccgtgtctc tgccggtgtg gcagccgaaa
    10141 tgacagaacg tggcctggcc agaatgcaat aacgggaggc gctgtggctg atttcgataa
    10201 ccagttcgat gctgccattg cccgcgccga tgaaacgata cgcgggtaca tgggaacgtc
    10261 agccaccatg acatccggtg agctgtccgg cgcagtaata cgtggtgttt ttgatgaccc
    10321 tgaaaatatc agctatgccg gacagggcgt gcgcgttgaa ggctccagcc cgtccctgtt
    10381 tgtccggact gatgatgtgc ggcaactgcg gcgtggagac acgctgacca tcggtgagga
    10441 aaacttctgg atagaccggg tttcgccgga tgatggtgga agctgtcatc tctggcttgg
    10501 gcggggcgta ccgcctgccg ttaaccgtcg ccgctgaaag ggggatgtat ggccataaaa
    10561 ggtcttgagc aggccgttga aaacctcagc cgtatcagca gaacggcggt gcccggtgcc
    10621 gccgcaatgg ccattaaccg cgttgcttca tccgcgatat cgcagtcggc gtcacaggtt
    10681 gcccgtgaga caaaggtacg ccggaaactg gtaaaggaaa gggccaggct gaaaagggct
    10741 acggtcaaaa atccgcaggc cagaatcaag gttaaccggg gggatttgcc cgtaataaag
    10801 ctgggtaacg cgcggattgt cctgtcccga cgcaggcgtc gtaaaaaggg gcagcgttca
    10861 gccctgaaag gtggcggcag cgtgcttgtg gtgggtaacc gtcgtattcc cggcgcgttt
    10921 attcagcaac tgaaaaatgg ccggtggcat gtcatgcagc gtgtggccgg gaaaaaccgt
    10981 taccccattg atgtggtgaa aatcccgatg gcggtgccgc tgaccacggc gtttaaacaa
    11041 aatattgagc ggatacggcg tgaacgtctt ccgaaagagc tgggctatgc gctgcagcat
    11101 caactgagga tggtaataaa gcgatgaaac atactgaact ccgtgcagcc gtactggatg
    11161 cactggagaa gcatgacacc ggggcgacgt tttttgatgg tcgccccgct gtttttgatg
    11221 aggcggattt tccggcagtt gccgtttatc tcaccggcgc tgaatacacg ggcgaagagc
    11281 tggacagcga tacctggcag gcggagctgc atatcgaagt tttcctgcct gctcaggtgc
    11341 cggattcaga gctggatgcg tggatggagt cccggattta tccggtgatg agtgatatcc
    11401 cggcactgtc agatttgatc accagtatgg tggccagcgg ctatgactac cggcgcgacg
    11461 atgatgcggg cctgtggagt tcagccgatc tgacttatgt cattacctat gaaatgtgag
    11521 gacgctatgc ctgtaccaaa tcctacaatg ccggtgaaag gtgccgggac cacactgtgg
    11581 gtttataagg ggaacggtga cccttatgcg aacccgcttt cagacgttga ctggtcgcgt
    11641 ctggctaaag ttaaagacct gacgcccggc gaactgaccg ctgagtccta tgacgacagc
    11701 tatctcgatg atgaagatgc ggactggact gcgaccgggc aggggcagaa atctgccgga
    11761 gataccagct tcacgctggc gtggatgccc ggagagcagg ggcagcaggc gctgctggcg
    11821 tggtttaatg aaggggatac ccgtgcctat aaaatccgct tcccgaacgg cacggtcgat
    11881 gtgttccgcg gctgggtcag cagtatcggt aaggcggtga cggcgaagga agtgatcacc
    11941 cgtacggtga aagtcaccaa cgtgggacgt ccgtcgatgg cagaagatcg cagcacggta
    12001 acagcggcaa ccggcatgac cgtgacgcct gccagcactt cggtggtgaa agggcagagc
    12061 accacgctga ccgtggcctt ccagccggag ggcgtaaccg acaagagctt tcgtgcggtg
    12121 tctgcggata aaacaaaagc caccgtgtcg gtcagtggta tgaccatcac cgtgaacggc
    12181 gttgctgcag gcaaggtcaa cattccggtt gtatccggta atggtgagtt tgctgcggtt
    12241 gcagaaatta ccgtcaccgc cagttaatcc ggagagtcag cgatgttcct gaaaaccgaa
    12301 tcatttgaac ataacggtgt gaccgtcacg ctttctgaac tgtcagccct gcagcgcatt
    12361 gagcatctcg ccctgatgaa acggcaggca gaacaggcgg agtcagacag caaccggaag
    12421 tttactgtgg aagacgccat cagaaccggc gcgtttctgg tggcgatgtc cctgtggcat
    12481 aaccatccgc agaagacgca gatgccgtcc atgaatgaag ccgttaaaca gattgagcag
    12541 gaagtgctta ccacctggcc cacggaggca atttctcatg ctgaaaacgt ggtgtaccgg
    12601 ctgtctggta tgtatgagtt tgtggtgaat aatgcccctg aacagacaga ggacgccggg
    12661 cccgcagagc ctgtttctgc gggaaagtgt tcgacggtga gctgagtttt gccctgaaac
    12721 tggcgcgtga gatggggcga cccgactggc gtgccatgct tgccgggatg tcatccacgg
    12781 agtatgccga ctggcaccgc ttttacagta cccattattt tcatgatgtt ctgctggata
    12841 tgcacttttc cgggctgacg tacaccgtgc tcagcctgtt tttcagcgat ccggatatgc
    12901 atccgctgga tttcagtctg ctgaaccggc gcgaggctga cgaagagcct gaagatgatg
    12961 tgctgatgca gaaagcggca gggcttgccg gaggtgtccg ctttggcccg gacgggaatg
    13021 aagttatccc cgcttccccg gatgtggcgg acatgacgga ggatgacgta atgctgatga
    13081 cagtatcaga agggatcgca ggaggagtcc ggtatggctg aaccggtagg cgatctggtc
    13141 gttgatttaa gtctggatgc ggccagattt gacgagcaga tggccagagt caggcgtcat
    13201 ttttccggta cggaaagtga tgcgaaaaaa acagcggcag tcgttgaaca gtcgctgagc
    13261 cgacaggcgc tggctgcaca gaaagcgggg atttccgtcg ggcagtataa agccgccatg
    13321 cgtatgctgc ctgcacagtt caccgacgtg gccacgcagc ttgcaggcgg gcaaagtccg
    13381 tggctgatcc tgctgcaaca gggtggtcag gttaaggact ccttcggcgg gatgatcccc
    13441 atgttcaggg ggcttgccgg tgcgatcacc ctgccgatgg tgggggccac ctcgctggcg
    13501 gtggcgaccg gtgcgctggc gtatgcctgg tatcagggca actcaaccct gtccgatttc
    13561 aacaaaacgc tggtcctttc cggcaatcag gcgggactga cggcagatcg tatgctggtc
    13621 ctgtccagag ccgggcaggc ggcagggctg acgtttaacc agaccagcga gtcactcagc
    13681 gcactggtca aggcgggggt aagcggtgag gctcagattg cgtccatcag ccagagtgtg
    13741 gcgcgtttct cctctgtatc cggcgtggag gtggacaagg tcgctgaagc cttcgggaag
    13801 ctgaccacag acccgacgtc ggggctgacg gcgatggcac gccagttcca taacgtgacg
    13861 gcggagcaga ttgcgtatgt tgctcagttg cagcgttccg gcgatgaagc cggggcattg
    13921 caggcggcga acgaggccgc aacgaaaggg tttgatgacc agacccgccg cctgaaagag
    13981 aacatgggca cgctggagac ctgggcagac aggactgcgc gggcattcaa atccatgtgg
    14041 gatgcggtgc tggatattgg tcgtcctgat accgcgcagg agatgctgat taaggcagag
    14101 actgcgttta agaaagcaga cgacatctgg aatctgcgca aggatgatta ttttgttaac
    14161 gatgaagcgc gggcgcgtta ctgggatgat cgtgaaaagg cccgtcttgc gcttgaagcc
    14221 gcccgaaaga aggctgagca gcagactcaa caggacaaaa atgcgcagca gcagagcgat
    14281 accgaagcgt cacggctgaa atataccgaa gaggcgcaga aggcttacga acggctgcag
    14341 acgccgctgg agaaatatac cgcccgtcag gaagaactga acaaggcact gaaagacggg
    14401 aaaatcctgc aggcggatta caacacgctg atggcggcgg cgaaaaagga ttatgaagcg
    14461 acgctgaaaa agccgaaaca gtccggcgtg aaggtgtctg cgggcgatcg tcaggaagac
    14521 agtgctcatg ctgacctgct gacgcttcag gcagaactcc ggacgctgga gaagcatgcc
    14581 ggagcgaatg agaaaatcag ccagcagcgc cgggatttgt ggaaggcgga gagtcagttc
    14641 gcggtactgg aggaggcggc gcaacgtcgc cagctgtctg cacaggagaa atctctgctg
    14701 gcgcataaag atgagacgct ggagtacaaa cgccagctgg ctgcacttgg cgacaaggtc
    14761 acctatcagg agcgcctgaa cgcgctggcg cagcaggcgg ataaattcgc acagcagcaa
    14821 cgggcaaaac gggccgccat tgatgcgaaa agccgggggc tgactgaccg gcaggcagaa
    14881 cgggaagcca cggaacagcg cctgaaggaa cagtatggcg ataatccgct ggcgctgaat
    14941 aacgtcatgt cagagcagaa aaagacctgg gcggctgaag accagcttcg cgggagctgg
    15001 atgacaggcc tgaagtccgg ctggagtgag tgggaagaga gcgccacgga cagtatgtcg
    15061 caggtaaaaa gtgcagccac gcagaccttt gatggtattg cacagaatat ggcagcgatg
    15121 ctgaccggca gtgagcagaa ctggcgcagc ttcacccgtt ccgtgctgtc catgatgaca
    15181 gaaattctgc ttaagcaggc aatggtgggg attgtcggga gtatcggcag cgccattggc
    15241 ggggctgttg gtggcggcgc atccgcgtca ggcggtacag ccattcaggc cgctgcggcg
    15301 aaattccatt ttgcaaccgg aggattcaca ggaaccggcg gcaaatatga gccagcgggg
    15361 attgttcacc gtggtgaatt tgtcttcacg aaggaggcaa ccagccggat tggcgtgggg
    15421 aatctctacc ggctgatgcg cggctatgcc accggtggtt atgtcggtac accgggcagt
    15481 ctggctgaca gccggtcgca ggcgtccggg acgtttgagc agaataacca tgtggtgatt
    15541 aacaacgacg gcacgaacgg gcagataggt ccggctgctc tgaaggcggt gtatgacatg
    15601 gcccgcaagg gtgcccgtga tgaaattcag acacagatgc gtgatggtgg cctgttctcc
    15661 ggaggtggac gatgaaaacc ttccgctgga aagtgaaacc cggtatggat gtggcttcgg
    15721 ccccttctgt aagaaaggtg cgctttggtg atggctattc ccagcgagcg cctgccgggc
    15781 tgaatgccaa cctgaaaacg tacagcgtga cgctttctgt cccccgtgag gaggccacgg
    15841 tactggagtc gtttctggaa gagcacggtg gctggaaatc ctttctgtgg acgccgcctt
    15901 atgagtggcg gcagataaag gtgacctgcg caaaatggtc gtcgcgggtc agtatgctgc
    15961 gtgttgagtt cagcgcagag tttgaacagg tggtgaactg atgcaggata tccggcagga
    16021 aacattgaat gaatgcaccc gtgcggagca gtcggccagc gtggtgctct gggaaatcga
    16081 tctgacagag gtcggtggag aacgttattt tttctgtaat gagcagaacg aaaaaggtga
    16141 gccggtcacc tggcaggggc gacagtatca gccgtatccc attcagggga gtggttttga
    16201 actgaatggc aaaggcacca gtacgcgccc cacgctgacg gtttctaacc tgtacggtat
    16261 ggtcaccggg atggcggaag atctgcagag tctggtcggc ggaacggtgg tccggcgtaa
    16321 ggtttacgcc cgttttctgg atgcggtgaa cttcgtcaac ggaaacagtg acgccgatcc
    16381 ggagcaggag gtgatcagcc gctggcgcat cgagcagtgc agcgaactga gcgcggtgag
    16441 tgcctccttt gtactgtcca cgccgacgga aacggatggc gctgtttttc cgggacgtat
    16501 catgctggcc aacacctgca cctggaccta tcgcggtgac gagtgcggtt atagcggtcc
    16561 ggctgtcgcg gatgaatatg accagccgac gtccgatatc acgaaagata aatgcagcaa
    16621 atgcctgagt ggctgtaagt ttcgcaataa cgtcggcaac tttggcggct tcctttccat
    16681 taacaaactt tcgcagtaat cccatgacag agacagaatc agcgattctg gcgcacgccc
    16741 ggcgatgtgc gccagcggag tcgtgcggct tcgtggtaag cacgccggag ggggaaagat
    16801 attttccctg cgtgaatatt tccggtgagc cggaggatta tttccggatg gctccggagg
    16861 actggctgca ggccaaaatg cagggtgaga ttgtggcgct ggtccacagt caccccggtg
    16921 gtctgccctg gctgagtgag gccgaccggc ggctgcaggt gcagagtgat ttgccgtggt
    16981 ggctggtctg ccggggagtg attcataagt tccgctgtgt gccgcatctc accgggcggc
    17041 gctttgagca cggggcgacg gactgttaca cgctgttccg ggatgcttat catctggctg
    17101 ggattgagat gccggatttt catcgcgggg atgactggtg gcgtcacggt cagaatctct
    17161 atcttgacaa tatggaggcg actggttttt accgtgtcgc actgacagag gcgcagccgg
    17221 gcgatgtgct gctgtgctgc tttggttcat cggtgccgaa tcatgccgct atttactgcg
    17281 gtgacggcga gctgctgcac catattcctg aacaactgag caaacgagag aggtataccg
    17341 acaaatggca gcgacgcaca cactccctct ggcgtcaccg ggcatggcgc gcatctgcct
    17401 ttacggggat ttacaacgat ttggccgccg catcgacctt cgtgtgaaaa cgggggctga
    17461 agccatccgg gcactggcca cacagctccc ggcgtttcgt cagaaactgt gcgacggctg
    17521 gtatcaggta cggattgccg ggcgggacgt cagcacgtcc gggttaacgg tgcagttaca
    17581 tgagactctg cctgatggcg ctgtgattca tattgttccc agagtcgccg gggccaagtc
    17641 aggtggcgta ttccagattg tcctgggagc tgccgccatt gccggatcat tctttactgc
    17701 cggagccacc cttgcagcat ggggggcagc cattggggcc ggtggtatga ccggcatcct
    17761 gttttctctc ggtgccagta tggtgct
//
LOCUS       DAZFKF010000103        17527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFKF010000103 DAZFKF010000000
VERSION     DAZFKF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.103"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            191..1402
                     /gene="mhpT"
                     /locus_tag="ACHKA1_004120"
     CDS             191..1402
                     /gene="mhpT"
                     /locus_tag="ACHKA1_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO5681693.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLTSLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            1504..2043
                     /locus_tag="ACHKA1_004121"
     CDS             1504..2043
                     /locus_tag="ACHKA1_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO5681694.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(2269..3102)
                     /gene="fghA"
                     /locus_tag="ACHKA1_004122"
     CDS             complement(2269..3102)
                     /gene="fghA"
                     /locus_tag="ACHKA1_004122"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO5681695.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMNHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(3195..4304)
                     /gene="frmA"
                     /locus_tag="ACHKA1_004123"
     CDS             complement(3195..4304)
                     /gene="frmA"
                     /locus_tag="ACHKA1_004123"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO5681696.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(4339..4614)
                     /gene="frmR"
                     /locus_tag="ACHKA1_004124"
     CDS             complement(4339..4614)
                     /gene="frmR"
                     /locus_tag="ACHKA1_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO5681697.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(4774..5820)
                     /gene="fbpC"
                     /locus_tag="ACHKA1_004125"
     CDS             complement(4774..5820)
                     /gene="fbpC"
                     /locus_tag="ACHKA1_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFO5681698.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPAQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADTA"
     gene            complement(5832..7910)
                     /locus_tag="ACHKA1_004126"
     CDS             complement(5832..7910)
                     /locus_tag="ACHKA1_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5681699.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFSEATREQRSRHYFDAGWALLCMAFIVIS
                     ATVVGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGEFAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(7979..9010)
                     /locus_tag="ACHKA1_004127"
     CDS             complement(7979..9010)
                     /locus_tag="ACHKA1_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5681700.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(9007..10323)
                     /gene="uhpC"
                     /locus_tag="ACHKA1_004128"
     CDS             complement(9007..10323)
                     /gene="uhpC"
                     /locus_tag="ACHKA1_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO5681701.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(10396..11937)
                     /locus_tag="ACHKA1_004129"
     CDS             complement(10396..11937)
                     /locus_tag="ACHKA1_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO5681702.1"
                     /translation="MSRNLRHWVISLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQIVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMHERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(11937..12566)
                     /locus_tag="ACHKA1_004130"
     CDS             complement(11937..12566)
                     /locus_tag="ACHKA1_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFO5681703.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRVQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            12869..13831
                     /gene="tauA"
                     /locus_tag="ACHKA1_004131"
     CDS             12869..13831
                     /gene="tauA"
                     /locus_tag="ACHKA1_004131"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5681704.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            13844..14611
                     /gene="tauB"
                     /locus_tag="ACHKA1_004132"
     CDS             13844..14611
                     /gene="tauB"
                     /locus_tag="ACHKA1_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO5681705.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            14608..15435
                     /gene="tauC"
                     /locus_tag="ACHKA1_004133"
     CDS             14608..15435
                     /gene="tauC"
                     /locus_tag="ACHKA1_004133"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO5681706.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            15432..16283
                     /gene="tauD"
                     /locus_tag="ACHKA1_004134"
     CDS             15432..16283
                     /gene="tauD"
                     /locus_tag="ACHKA1_004134"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO5681707.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPLQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLSFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(16390..17364)
                     /gene="hemB"
                     /locus_tag="ACHKA1_004135"
     CDS             complement(16390..17364)
                     /gene="hemB"
                     /locus_tag="ACHKA1_004135"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO5681708.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         4167 a         4655 c         4774 g         3931 t
ORIGIN      
        1 ggcgcgggaa tttgtaggcc tgataagacg cgcaagcgtc gcatcaggca tctgcacacg
       61 actgccggat gcggcgtgaa cgccttatcc ggcctacatt tcgcccgtag gcagtcatta
      121 aatagttctg attacgcggc tggcaatgta tcagtcgcga ttcacctgca ctcgcaacga
      181 ggttcatctt atgtcgactc gtaccccttc atcatcttca tcccgcctga tgctgaccat
      241 cgggctttgt tttttggtcg ctctgatgga agggctggat cttcaggcgg ctggcattgc
      301 ggcgggtggc atcgcccagg ctttcgcact cgataaaatg caaatgggct ggatattcag
      361 cgccggaata ctcggtttac tacccggcgc gttggttggc ggaatgctgg cggaccgtta
      421 tggtcgcaag cgcattttga ttggctcagt tgcgctgttt ggtttgttct cgctggcaac
      481 ggcgattgcc tgggatttcc cctcactggt ctttgcgcgg ctgatgaccg gtgtcgggct
      541 gggggcggcg ttgccgaatc ttatcgccct gacgtctgaa gccgcgggtc cacgttttcg
      601 tgggacggca gtgagcctga tgtattgcgg tgttcccatt ggcgcggcgc tggcggcgac
      661 actgggtttc gcgggggcaa acttagcatg gcaaacggtg ttttgggtag gtggtgtggt
      721 gccgttgatt ctggtgccgc tattaatgcg ctggttgccg gagtcggcgg ttttcgctgg
      781 cgaaaaacag gctgcgccac cactgcgtgc gttatttgcg ccagaaacgg caaccgcgac
      841 gctgctgctg tggctgtgtt atttcttcac tctgctggtg gtttacatgt tgatcaactg
      901 gctaccgctg cttttggtgg agcaaggatt ccagccatcg caggcggcag gggtgatgtt
      961 tgcactgcaa atgggtgcgg cgagcgggac gttaatgttg ggcgcattga tggataagct
     1021 gcgtccagta accatgtcgc tactgattta tagcggcatg ttaacttcgc tgctggcgct
     1081 tggaacggtg tcgtcattta acggtatgtt gctggccgga tttgtcgcgg ggttgtttgc
     1141 gacaggtggg caaagcgttt tgtatgccct ggcaccgttg ttttacagtt cgcagatccg
     1201 cgcaacaggt gtgggaacag ccgtggcggt agggcgtctg ggggctatga gcggtccgtt
     1261 actggccggg aaaatgctgg cattaggcac tggcacggtc ggcgtaatgg ccgcttctgc
     1321 accgggtatt cttgttgctg ggttggcggt ttttattttg atgagtcgga gatcaagaat
     1381 gcagccgtgt gccgatgcct gacgcgcctt attaagcagg ggcgaaaggg acttgccccc
     1441 aatacaccca cggcgtttac aggtatactc gctaaaaatt attcagcggg tttggaaaca
     1501 aagatggcaa aacttacctt acaagagcag ttgctcaaag caggattagt caccagcaaa
     1561 aaagcggcga aggtcgagag aacggcgaaa aaatcgcgcg ttcaggcgcg tgaagctcgg
     1621 gcggcggtag aagaaaataa aaaggcacag cttgagcgtg ataaacagct tagcgaacag
     1681 caaaaacaag cggcgttggc aaaagaatat aaagctcagg tgaagcagct tattgaaatg
     1741 aaccggatca ccattgccaa tggcgatatt ggttttaact tcactgatgg caatctgatt
     1801 aagaagattt ttgtcgataa gctcacacaa gcgcagttga ttaatggtcg tctggcgatt
     1861 gcccgcttgt tggtcgataa caatagcgaa ggtgaatacg ccattattcc cgccagcgtt
     1921 gccgataaaa ttgctcagcg tgatgccagc agtattgtgt tgcacagcgc gctcagtgcc
     1981 gaagagcagg atgaagacga tccgtatgcc gacttcaaag tgcctgatga tttgatgtgg
     2041 taaacataga taagccgtgc gcaaatgccc gatgcgacgc tatgcgcgtc ttatcatgcc
     2101 tatggtttat gggcgaagtg taggccggat aaggcgttca cgccgcatcc ggcagccgtg
     2161 cgcagatgcc tgatgcgacg cttacgcgtc ttatcatgcc tacggtttat gggcgaagtg
     2221 taggccggat aaggcgttca cgccgcatcc ggcagtcgtg cactattatc aacgcatatt
     2281 cagtttattg gcgtggtagg caatatgctc gccaataaaa ctggagacaa aataatagct
     2341 gtggtcgtaa ccctcgtgat aacggattaa cgtcttgata ttcatctcct ggcagatctt
     2401 ttcaagattt ggagtccgca gctgttctgc gtaaaaatca tcactcaacc cctgatcaac
     2461 catgatttcc gcaacgcgtt gaccttgtga aataagactc accggatcgt aatccaacca
     2521 ggcatcttta ttttcaccaa gatatgcggc aaaggcttgc tgtccccacg gtacttgcga
     2581 tggggagaca atgggcgaaa acgccgagac gctgacatat tcatctgggt tacgtaacgc
     2641 cagcaccagc gcgcccagtc cgcccataga atgaccagag atagactttt tggccgttgc
     2701 cggaaaatga ttcatcacta aatccggcag ctcgttgcgg atatagtcat acattttgta
     2761 atgttcattc cacggcgctt gcgtcgcgtt caggtaaaac ccggcacctt gcccgagatc
     2821 gtaacggtca gcatctgcga catgactgcc tcgcggactg gtgtccggcg caacaacaat
     2881 aatgttgtgc tcagccgcgt aacgctgcat ccccgattta gtaatgaaat tctgctcgtt
     2941 gcaggtcagg cctgaaagcc agtacagcac cggcaatttt tcattcgcgg cttttggtgg
     3001 gagatagacg ccgacattca tttcacattt cagtgatgcg gaataatgcc gatacacatt
     3061 ttgccagccg ccaaagctgg catgtttttc aatgagttcc atcagtcatt atctcacggt
     3121 taatccccca gacggctgcc cggggagagt attaagaacc ccgtctgaag tggacggggt
     3181 aaaccagcgg gaaatcagta acgaattacg gttcgaatgg atttgccttc atgcatcagg
     3241 tcgaaggcgt cattaatttc atccaggctc atggtatgcg tgacaaacgg ttccagatcg
     3301 atatcacctt tcatcgcatc ttcaaccata cccggtaact gggaacgacc tttcacgccg
     3361 ccaaacgcgg aacctttcca tacgcgacca gtgaccaact ggaatggacg ggtggagatt
     3421 tcctgaccgg aacccgcgac cccgatgatc accgactgac cccagccgcg gtgcgcactt
     3481 tccagcgccg cacgcatcac gttgacatta ccgatgcatt caaaggtatg gtcgataccc
     3541 catttgttga tatccagcag gacatcttta atcggtttgt cgtagtcatt cgggttaatg
     3601 cagtcggtgg caccgaagcg acgcgccaga tcgaatttct tcgggttggt atcgatagcg
     3661 ataatccgtc ccgctttcgc ctgacgcgcg ccctgaacca ctgccagacc aatcgcgcca
     3721 agaccaaata cggcaacaga atcacctggc tggactttag ctgtgttgtg taccgcgcca
     3781 ataccggtgg tcacgccaca gcccagcagg cagacgtgtt catggtttgc ttctggatta
     3841 attttggcca gagacacttc cgcgaccacg gtgtattcac tgaatgtaga gcaccccatg
     3901 tagtgataaa gcggctgccc gttgtaagaa aaacgggtgg tgccgtctgg catcaggcct
     3961 ttaccctggg tttcgcgaac cgcaacacag aggttagttt tgccagaacg acagaactca
     4021 cactcgccgc actctgcggt gtaaagcggg atcacatggt cgcctggttt gacgctggtt
     4081 acgccttcac cgacttcaac cacaacgcca gccccttcgt gaccgagaac caccgggaat
     4141 acaccttccg ggtcatcgcc ggagagggta aatgcgtcgg tatggcaaac gccggtatgg
     4201 gtgactttaa tcagcacttc accttttttc ggtggtgcaa cgtcaatttc aacgatttcc
     4261 agcggtttac cgggagcaaa tgcaacggca gcacgtgatt tcatctctcg ctcttcctca
     4321 atatagtaat agattcagct atttaagata ggcacgaacc agttcaatag tgtcgtcaac
     4381 ggattggctg acttcgcggc tgtagcagtc atttcggtca aacgtttccc ggatatggct
     4441 ttcaagcact tctgccatca gcccattagc cgcgccccga acggcagcga tctgttggag
     4501 tatggcacgg cattcggcat caccctccag cgaccgttcc agagcatcaa tctgcccccg
     4561 aatacgacga actcgagtaa ggaccttttt cttctcttcc ggagtactgg gcatttcgca
     4621 cctcatcatc tgcacgcaat atactatagg ggggtatgct atatgtcaat gcataccccc
     4681 ctatagtata taacactgga gaataattgt tgtccgaagc ctaaccaggt gaacggtccc
     4741 tcaagacaga tggggaccgt ttatgtgcta tttttatgcc gtatccgcta aaacaaacat
     4801 cccgtaagga tggatttcga ggtaatagtg ctcaccaata tcgggctgta aacgggtggc
     4861 gtttacttgt aataaaatct cctgtccatg ccactctacg gtcacttcgt actgcggccc
     4921 catgtaggcg acatggcgaa taacacaacg ctgactctct tcgccgtgat tgcttaaggt
     4981 gatggcttcc gggcgaacgc cgacggtgcc gctaccttgc gccgggaaat gcgccgcgcg
     5041 cggcaggcga taaccgtaga tatcgacata ctcttcgcta aagttcgccg ggaacaggtt
     5101 ggcgtcgccc ataaaacttg ccataaatcg ggaggctggc tgacgataga gatcctgcgg
     5161 tgagccaatc tgcatgatgt gacctttatt cattaccagc acagtgtcgg acaccgcgaa
     5221 agcttcactt tgatcgtgag tgacgtagag cgacgtgata ttaaactgct tttgcagctc
     5281 gcggatctta tcgcgcatac tgcggcgcag gttggcatcg aggttactta acggctcatc
     5341 aaatagcagc acctttggct tgaggatcaa cgcgcgggcc agtgccacac gctgttgctg
     5401 accaccggaa atctgatcga catagcggtc ctcgaagcct tccagatcca ccattgccag
     5461 cgcctccttc acccgctgtt tcacttcgct acgcgacacg ccaagcatct tcagcccgta
     5521 gccaacgttt tcacccagcg acatatgcgg gaacagagcg tatgactgaa acaccatgca
     5581 gatatcgcgc tgttgaatgg aacgatgcgt gacatcttcg ccatcaataa aaatttgccc
     5641 ttcgctcggt ttttccagcc cggcaaccag acgcaaaatg gtggttttgc cacagccgga
     5701 aggaccgagc agcgtcacca tttgcccttg tggaatggtg agattgatat tgtcgataac
     5761 cgtattactg ccgaatcgtt tagtgacgtt gcgcagttca acaaaatttt tctgactcat
     5821 agtgcgctcc attacgcctg gtttttggct ttagaacggg agatacgagc ttctccgatc
     5881 agccagtcaa agaggaaaat aatcgccagc atcaccacga tcagaatcga accataggcg
     5941 atggctacgc catattcgcc gtcttcaacg cggttaagaa tgtaggcggt tgccacgcgg
     6001 gtgtccggcg tgacgagaaa gacgatggcg ctgaccgtcg tgatggcccg cacaaagctg
     6061 tagatcaggg ccgagagaat ggccggacgc agcagcggca gtaaaatgtg ggttatggtt
     6121 cgcaaggaac ccgcgcgcag gctgagtgag gcttcatcca gcgatttgtc gatctgcccc
     6181 agacccgcaa tcccggcgcg aatacccacc ggaacgttac gcatcaccat cgagataatc
     6241 acaattgccg ccgttccggt gagataaacc ggagcgctgt taaaggcgag aatatacgag
     6301 acgcccgcca ccgtacccgg tacggcaaag cacagcatgg tggtgaactc gatggtcttt
     6361 ttgcctttga attgttgacg caccaccacc caggcgatga gcaggccaaa gatagcggta
     6421 atgggagcgg caatcccggc gtagagcagg gtatccagca gtgaaggcca cgcgccgtcg
     6481 ctcattccct gaccaaatag cttgataaag ttatccagcg tcagggtgta gtccacgccc
     6541 cagttcaccg tgaagctgcc gtagaaaatg ctgccgtaga gcagggcgtt aaaggcaatc
     6601 catatcgcaa gtatggcgac cacgctccac accagggtga ccggtagcgg ctggacgtcg
     6661 ccacggtagg atttgccgga aaccgtgacg taagagcgtt taccaatcca catatactgg
     6721 atgcagaaca ccagcagcga gaacagcagc aggaaagcgc caagggtgct ggcggcctga
     6781 taatccagct gcgagccggt aatatagaag taaatttgcg tcgccagtac gtcgaagtta
     6841 ccgccaagta ccagcgggtt actgaaatca gccagtgact gtaccacgac gattaaaaag
     6901 gcattggcga gtgcgggctt caacaacggc acaaacacgc cgttaaaggt ttgccagcgg
     6961 ctggcgcgca gggtgtagga ggcctcttcc agcgagggat gaatggtttt aatggccccg
     7021 tcgaggatca taaatgccat tggcgtgaat gccagaacct gtgccagcca gatcccggta
     7081 aaaccgtaca gccagttggt gttggtcagc ccaaaccact ccaccataaa ttccgtaaca
     7141 tagccggagc gccccatcat gagcgtcacg cctaagccga ccacaaacgg tggcgtaacg
     7201 atgggcaaaa tggaaaagat gcggccaata atggcgctgc gtttagcgat gcgcgtggta
     7261 taaatcgcca gcaccaggcc gaagaaagta cagcctatcc ccaccgctat cgacaagacg
     7321 atagagttga cgatcacctg aacaatatgt gcctgcgaga gcacggtcat aaacgccagc
     7381 ggggcgaact ctcccgcgtc gttggtgaac atcgggataa aaatggcgat gctcggccag
     7441 acgataaaaa tgccaatcag cgccacaatc gcaatcagtg agccgatcac gaagcggtcg
     7501 ccacccagcc attcgaggcg ggttaaggcc agcgtcataa tcgcgcctag ttcgacaaac
     7561 agcacgatgg cggcgtagcc taaaccgcgt ccaaccacgg tggcgctgat gacgataaac
     7621 gccatacaga gcaatgccca gccggcatcg aaataatgac ggctacgttg ttcgcgcgtg
     7681 gcctcactaa acgggcggac cagcaacagg ctcgggagta aataccacag ccagctgata
     7741 ttaaaatgcg accagccgta cgccccgagg atctcatcac tcgtggactc cagcaggcca
     7801 taatcgaggc tccagctcgg caacaggaca aatgccagcc aacccagcaa aacccaaagg
     7861 aaaatcgcat cccgcttttt cacgggatgg agtgcaagtg tctgtgacat agcagttccg
     7921 gtatggttgg ccggggcagg cgtctgcgct gcacccggca aatgcgccta atgcgggttt
     7981 atttacccat ttttacttcg ctaacccatt tattaatcaa tgccttacga acatccgtgg
     8041 aaccgtattt gtccatgtcg tagttgatta atttcaggtc gtcgagcttc agtgagtttg
     8101 gcgacgtttc ggcggtggta ttggtcagga tctgataaga cttccctttc ttccacgcca
     8161 gttcctgcgc ttcttttgat aatacccagt ccacaaacag tttggcgttg tcgaggttgc
     8221 gcgcgccttt cagaatgctg acgccgccaa tttcgtagcc tgtaccttca cacggggaaa
     8281 tcaactccag cggcgcgccc tgttcttttt ccagcgagta gtcatgcagg aagccaatac
     8341 caatcgccgt ttcgccacgg gcggcgttac gtgccggggc tatgcctgat ttggtgtact
     8401 gagagacgtt ggcgttcagt tgttttagat aatcaaaggc ctgatcttcc ccccacagct
     8461 gcgcaaaagt agccagggcg gtgtaggcag taccggaact ttgcggatcg gcaatttgaa
     8521 tttcgccttt atattccggt ttggtgagat ctttccagca cttcggcacc ggcaggtttt
     8581 tctctttcag acgatcggtg tttacgccaa aaccgaggat ccccacataa acggcagagg
     8641 acagattccc tttcactttt gccggatcgc ggaatttctc catgatctgc tcaaggttag
     8701 gggatttata cgcctgtagc aggcccatct cgccagcctg cgattgcggg tctaaagtgc
     8761 cgccgtacca gacgtcagcc tgggggtttt tcttttcagc atcgactttt gccagggtgc
     8821 ttccggaacc gttgcggata aacgaggttt ttacatcata tttctcgcca aacgctttgg
     8881 tttcggcctc acacatttca ttggtggcgc tgcaatagac caccagccgg ccttttgcct
     8941 gcgcggcacc cgttaaagtc gccagcgcaa cggcagaagc aatcagagta gaggtaaagg
     9001 tcattttcat tgttttatcc ttcgagcgtg agtggtggtg atgcccgcca tcagtaacgg
     9061 catcagcagt aaacccataa gtactgcggc gaccgagagt aaactgaaca ttcccggcca
     9121 gccgtagcgt tcgatgacca gcgacagcgg ccagcctgcc agcgccgcgc caaggtaagc
     9181 gaacaggccg agaaatccgg tgattgaacc cgccgcagct ttgtgcccac actccaccgc
     9241 cgcaagtcca atcagcattt gtgggccaaa gacgaaaaag cccacggtaa aaaagcacac
     9301 tgccagcaga gcgtagtgat gaaccggtgc cagccacagt gcggcgactg acaccatcaa
     9361 ccccaacgtg aacagcaaaa tcattggcgc tcgttgcccg ctaaacagca aatccgatcc
     9421 ccatccggca aataacgcgc cgagtaagcc gcccacttca aacagcatta ccgtggcgtt
     9481 ggcgctgagc aggttgacac cgtggctttc cgtcagccag atattgcccc agtcgttaag
     9541 cgcaatccgg atcacataaa ccagtacata agagacgccc agtagccaga tcagcggatt
     9601 ttgcagcatg gtggtgcgca acatttgcca cagccccatc ggcggacttt gttgttcctg
     9661 acgtaactcc agcggatcgt gacgccagtg accaactgtc ggtaatccct cttcctgggg
     9721 cgtgcctttg agttgtagcg ttaaccagat ccctaatgcc atgctgatta tccccggtgt
     9781 cagcatcgcg gcctgccagc cccaccagtg ggcggcaaag gcgctaatca gcggaataat
     9841 cgctccgcca atgttgatcg acatattcca gcagccccac cagaaaccgc gctcattgcg
     9901 tgagtaccag tgggtcagca agcgggcaca cggtggccat ccccagccct gaaaaaaacc
     9961 gttcagtgtc cagaccacaa gcagaagcgt aagtgattcg ccaaaggcaa acaccacgtt
    10021 caataacccg gtggcgaaca agccgacgcc cataaaaccg cgttgtccgt ggctgtcatg
    10081 ccacagtccg gcggtaaatt tcgacaatcc gtagctgaga taaaacagcg aacccagcag
    10141 gccaatgtcg cctttatcca gtccgaggtc ggtttgtagc gccggaagga cgtaattcac
    10201 gctttttcgc gtcagataaa atgcggcgta gccaataatc atgcacatca gcaatcgcgg
    10261 acgtaatgtc cggtagcatt gattaatttc tctcgctgaa cgcgcgtgca tggttgtctc
    10321 catgttgctg actatatgaa aatgaaagtg aaaggggatg agagaaaatc cgggatacct
    10381 aagacttttt cctggttatc cggcgatttg ttgcaaaaat gtgggcaagt taacaattac
    10441 ccgcgtgccg tgctgttttt cgagcgttaa gtcgccgccc agggcgctga cgcgctcatg
    10501 catcccctga atgccaaatc cggccatttt cccgctggcg atccccacac cgttgtcgct
    10561 gacttccaga tgcaatacct ctccctgctg gcgcaggata atcgtcactt cgctggcttc
    10621 ggcgtgttta cagatgttat taagcaactc ctgcaataac cgatacaagg taaagcgcac
    10681 cgtttcgttt tctggtgtgg agttcagttg ataggcaaac tggcaatgaa tgccgcgttc
    10741 actaaaggca aattcattaa tcagatgcaa caacgcttcg cgaaatgtca gttcatcaag
    10801 cgcgggaggc cgcaactggc gcagcaattg gcgggtggag agatgaatgc ggcgggcgag
    10861 ctcattgatt tggctggctg cggattgaat ttgcgccgga tcacgcgccc gcttcaccag
    10921 ttgcgactgg atctgaatgg cggtgatgtt ctgaccgatt tcgtcatgca gctctcgcgc
    10981 caggtttttt cgcgtgtcct cctcggtgtg gatcagcttt tccgccaatg cccgccgcgc
    11041 tgccagctcg gcctccagcc gttgtcggta atggtgcagg tttagcgcca gatgttgctg
    11101 gcgactgatg gcaatgccca ggccaattcc cagcaaggcc tgggtcgcga ggaaaatctc
    11161 cagctccacc aggttactaa agcccacgcc aatctggcgg gcgatggtga tcatcatgct
    11221 gcccaaaagc ccggagagta cgccaccttg ccagccaaat ttccacgcca tcaccacatt
    11281 gggcaaaaag accacgatca acagcagacg ttcaatttca ggtgacagca ctatctgagt
    11341 gccgatacca ataataaaaa acaggctaca ccagatgatt aaagaggtac gcaagggggg
    11401 attatgcgta tcgagtccca ggagctggta gcgatgctgc tggcgcagaa actcaaagat
    11461 aagatagaca aacggcgtca gtaatacgcc gccggtaaat gaggtcaaac ccggcagcat
    11521 tattgggcct gacataaaag gggagagcag ggcggtattc aacaatgaag ctgccgtgac
    11581 agtggctaac agcaacgtca gacgctgcca gtaaagtgga aaacggtgcc agaagttttg
    11641 cgcaaggagg gcaggaaaca ggctaataac cggcgttgcc agcatgagat aaccgttcaa
    11701 tagctgttca ctatggagcc agaacatcat cagcaaaggg gggagcacca acgcaggcca
    11761 gtagcggcga gagagcaaaa taacaagggc gagataaact ccctgcggga gaaacaacac
    11821 cgcctgttga ccgttattgg tcagataaaa gctgagcgtc cacagcatca gccagccggt
    11881 tccccaggcc agcacaataa acagcgaaat cacccaatga cgaagattgc gcgacattaa
    11941 tgccccgcca gcagatggtg gtcgagcgca aaatgtacca gttcaatggt gctatggcag
    12001 ttcagttttc ccagcacatt ggcgcgatgc acatgtaccg ttttatgact gagttcgagt
    12061 ttaaaggcga tctctttaac gctgtctcct ttgacaagta aatcaaacac ttcacgttct
    12121 cgtggggtga gaatttccag cgcctgggcg ggttgcccgc cgccgcgtaa agcccgaatc
    12181 gcatcggcac agagataatg cccgcccagc ccgaccgagc gcaccgcctg tactaactct
    12241 tccggcccac agcgtttggt taaatacccg ctggcaccgg catccagcgc gctttgcaca
    12301 aaggttggcg cgtcataaat gctaagaata atggcgcgaa attgcggttt ttgtacacgc
    12361 aggcgcttaa gcaaactgag gccgttttcg tccggcatgg caatatcaat gacggcaaca
    12421 ttgacgtcat cacggattaa tgccgaccat gcctgcgcgg cactactgta ctgaccaatg
    12481 acctccagat cgtcttccag agagagtaat tgtgcgaagc cagagcgcac caccacatgg
    12541 tcatccacca gcactacccg aatcatactt ttcttctctt attatctaat tctcctatca
    12601 tgcatcacgg cgcggcgctg gcaatcaacc agcgacagat atggaatgga tgtaacaaaa
    12661 tggcaataat ttgccggagc gcgcacatct tatctggctt gtgaaccggc gggatacgtt
    12721 cgccaaaacc gcaaaatgcg caaactcgct aaccattgcc gatttttttc taaaaacgct
    12781 ttgttcttaa caagaccttt taattccttt atcaaactga cccgcaacga aatactgctc
    12841 ccataacaag acaggggagc agacaatcat ggcaatttca tcgcgtaaca cacttcttgc
    12901 cgcactggca ttcatcgctt ttcaggcaca ggcggtgaac gtcaccgtgg cgtatcaaac
    12961 ctcagccgaa ccggcgaaag tggctcaggc cgacaacacc tttgctaaag aaagcggagc
    13021 aaccgtggac tggcgtaagt ttgacagcgg agccagcatc gtgcgggcgc tggcttcagg
    13081 cgacgtgcaa atcggcaacc tcggttccag cccattagcg gttgcagcca gccaacaggt
    13141 gccgattgaa gtcttcttgc tagcgtcaaa actgggtaac tccgaagcac tggtggtaaa
    13201 gaaaactatc agcaaaccgg aagatctgat tggcaaacgc atcgccgtac cgtttatctc
    13261 cactacccac tacagcctgc tggcggcgct gaagcactgg ggtattaaac ctgggcaagt
    13321 ggagatagtt aacctgcagc cgcccgcgat tatcgctgca tggcagcggg gagacattga
    13381 tggtgcttat gtctgggcac cggcggttaa cgccctggaa aaagacggca aggtgttgac
    13441 cgattctgaa caggtcgggc agtggggcgc gccaacgctg gacgtctggg tggtgcgcaa
    13501 agattttgcc gagaaacatc ctgaggtcgt gaaagcgttc gctaaaagcg ccatcgatgc
    13561 tcagcaaccg tacattgcta acccagacgt gtggctgaaa cagccggaaa acatcagcaa
    13621 actggcgcgt ttaagcggcg tgcctgaagg tgacgttccg gggctggtga aggggaatac
    13681 ctatctgacg ccgcagcaac aaacggcaga actgaccgga ccggtgaaca aagcgatcat
    13741 cgacaccgcg cagtttttga aagagcaggg caaggtcccg gctgtagcga atgattacag
    13801 ccagtacgtt acctcgcgct tcgtgcaata aaaggaggcg cagatgctgc aaatctctca
    13861 tctttacgcc gattatggcg gcaaaccggc actggaagat atcaacctga cgctggaaag
    13921 cggcgagcta ctggtggtgc tggggccgtc cggctgcggt aaaaccaccc tgctgaatct
    13981 gattgccggt tttgtgcctt atcagcatgg cagcattcaa ctggcgggta agcgtattga
    14041 gggaccggga gcagagcgtg gcgtagtttt tcagaatgaa gggctactac cgtggcgcaa
    14101 tgtacaggac aacgtggcgt tcggcctgca attggcaggt atagagaaaa tgcagcgact
    14161 ggaaatcgcg caccagatgc tgaaaaaagt ggggctggaa ggcgcagaaa aacgctacat
    14221 ctggcagctt tccggtggtc aacgtcagcg ggtgggcatt gcccgcgcgc tggcggcgaa
    14281 tccccagctg ttattactcg acgaaccgtt tggtgcgctg gacgccttca cccgcgacca
    14341 gatgcaaacc ctgctgctga aactctggca ggagacgggc aagcaggtgc tgttgattac
    14401 ccacgatata gaagaagcgg tgtttatggc gactgaactg gttctgcttt catccggccc
    14461 tggacgtgtg ctggagcggc tgccgctcaa ctttgctcgc cgctttgttg cgggagagtc
    14521 gagccgcagc atcaagtccg atccacaatt catcgccatg cgcgaatatg ttttaagccg
    14581 cgtatttgag caacgggagg cgttctcatg agtgtgctca ttaatgaaaa actgcattcg
    14641 cggcggttga aatggcgctg gccgctctcg cgtcaggtga ccttaagcat tggcacgtta
    14701 gcggttttac tcactgtatg gtggacggtg gcggcgctgc aactgattag cccgctgttt
    14761 ttgccgccgc cgcaacaagt actggcaaaa ctgctcacca ttgccggacc gcagggattt
    14821 atggacgcca cgctgtggca gcatctggca gccagcctga cgcgcattgt gctggcgcta
    14881 ctcgcggcgg tgttgatcgg cattccggtc gggatcgcca tggggctaag cccgacggtg
    14941 cgcggcattc tggatccggt aatcgaactt tatcgtccgg tgccgccgct ggcttattta
    15001 ccactgatgg tgatctggtt tggtattggc gaaacctcga agatcttact gatctattta
    15061 gcgatttttg caccggtggc gatgtcggcg ctggcggggg tgaaaagcgc gcagcaggtt
    15121 cgcattcgtg ccgcccagtc gctgggtgcc agccgtgcgc aggtgctgtg gtttgtcatt
    15181 ttgcccggcg cgctgccgga aatcctcacc ggattacgca ttggtctggg ggtgggctgg
    15241 tctaccctgg tagcggcgga gctgattgcc gcgacgcgcg gtttaggatt tatggttcag
    15301 tcagcgggtg aatttctcgc gactgacgtg gtgctggcgg ggatcgcggt gattgcgatt
    15361 atcgcctttc ttttagaact gggtttgcgc gcgttacagc gccgactgac gccctggcat
    15421 ggagaagtac aatgagtgaa cgtctgagca ttaccccgct ggggccgtat atcggagcac
    15481 aaatttcggg tgccgacctg acgcgcccgt taagcgataa tcagtttgaa cagctttacc
    15541 atgcggtgct gcgccatcag gtggtgtttc tgcgcgatca agctattacg ccgctgcagc
    15601 aacgcgcgct ggcccagcgt tttggcgaac tgcatattca ccctgtttac cctcatgccg
    15661 aaggggttga cgagatcatc gtgctggata cccataacga taatccgcca gataacgaca
    15721 actggcatac cgatgtgaca tttattgaaa cgccacccgc aggggcgatt ctggcagcga
    15781 aagagttacc ttcgaccggc ggcgatacgc tctggaccag cggtattgcg gcctatgagg
    15841 cgctctctgt tcccttccgc cagctgctga gcgggctgcg tgcggagcat gattttcgta
    15901 aatcgttccc ggaatacaaa taccgcaaaa ccgaggagga acatcaacgc tggcgcgagg
    15961 cggtcgcgaa aaatccgccg ttgctacatc cggtggtgcg aacgcatccg gtgagcggta
    16021 aacaggcgct gtttgtgaat gaaggcttta ctacgcgaat tgttgatgtg agcgagaaag
    16081 agagcgaagc cttgttaagt tttttgtttg cccatatcac caaaccggag tttcaggtgc
    16141 gctggcgctg gcaaccaaat gatattgcga tttgggataa ccgcgtgacc cagcactatg
    16201 ccaatgccga ttacctgcca cagcgacgga ttatgcatcg ggcgacgatt cttggggata
    16261 aaccgtttta tcgggcgggg taatacgaga gtggacggtc ccctcgcccc cttggggaga
    16321 gggttagggt gagggggcgt tcaccgtact ttcaacaggt taactccccc tttctgagag
    16381 gaaacaaaat taacgcagaa tcttcttctc agccaaatcc agcgcaaagt agctgaaaat
    16441 cagatccgca cccgcacgct taatcgaacc taagctttcg agcaccactt tctcttcatc
    16501 tatagcaccc gccagcgcgg cgaacttaat catcgcgtac tcaccgctca cctgatacgc
    16561 gccaatcggc aattcagtac gttcacgcag ctcacgcacg atgtcgaggt acgctccagc
    16621 aggtttaacc atcaggcagt ctgcgccctg ggcttcatcc agcagtgatt cacgaatcgc
    16681 ctcgcgacgg ttcattgggt tcatctgata gcttttgcgg tcgcctttta atgcgcttcc
    16741 ggcagcttca cggaacggac cataaaagga ggaggcgaac ttggtcgaat acgacataat
    16801 cgccgtatct ttaaagccag cagcgtccag cgcctggcga atcgcctgta cctggccatc
    16861 catcgcggcg gaaggggcga tgaagtctgc acctgcagca gctgcaacca cggcttgctt
    16921 gcctaaattt tccagagtcg cgtcgttgtc gacgccatgc tcgcacagca caccgcagtg
    16981 accgtgagaa gtgtattcac agaagcaggt gtctgacatg acaatcattt ctggcactgt
    17041 ctgcttgcag atgcgcgaca tacgcgccac cagtccatct tcccgccagg catcgctgcc
    17101 ggtttcatcg gtatggtgag agatgccaaa agtcatcacg gaacgaatac cggcgttggc
    17161 gatgcgttca atttcgcgtg ccagatgttt ctctggaatg cgcatcacgc ctggcatggc
    17221 ttcaacggct ttgtagtcgt caatttcttc ttcaacaaag atcggcaaca ccaggtcgtt
    17281 aaggctaagt gttgtctctt caaacatagc gcgcagcgca ggagatttgc gcaggcgacg
    17341 agggcgttgg attaaatctg tcatggtctg cctgatgttt gtggaatcga ggggcatagt
    17401 atacctgaag cagggtaggg atgttttacg aaagttgtcg cgatgttgac aagaagagaa
    17461 tggaagagag gccaggacat ctttgtaata gaaatagcaa taacttcata ttcactgaaa
    17521 atatttt
//
LOCUS       DAZFKF010000104        17020 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFKF010000104 DAZFKF010000000
VERSION     DAZFKF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.104"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..419)
                     /locus_tag="ACHKA1_004136"
     CDS             complement(<1..419)
                     /locus_tag="ACHKA1_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001359473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5681709.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     ATQLPSFRQKLNEGWYQVRISGRDAGENELSARLNEPLANGAVIHVVPRLAGAKSGGV
                     FQAVLGAALIAVAWWNPVGWLGAAAVSGLYAAGASMIL"
     gene            complement(317..1060)
                     /locus_tag="ACHKA1_004137"
     CDS             complement(317..1060)
                     /locus_tag="ACHKA1_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5681710.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFV"
     gene            complement(1065..1763)
                     /locus_tag="ACHKA1_004138"
     CDS             complement(1065..1763)
                     /locus_tag="ACHKA1_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5681711.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSTSFVLSTPTETDG
                     SVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(1773..2102)
                     /locus_tag="ACHKA1_004139"
     CDS             complement(1773..2102)
                     /locus_tag="ACHKA1_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5681712.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            complement(2102..5167)
                     /locus_tag="ACHKA1_004140"
     CDS             complement(2102..5167)
                     /locus_tag="ACHKA1_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5681713.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKSAQAHERMAREVEKTRQRMEALSQK
                     MRDEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGDQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQISALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNELQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSSGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAGTAEQVFSSAFSYMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFAPQ
                     YNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            complement(5139..5468)
                     /locus_tag="ACHKA1_004141"
     CDS             complement(5139..5468)
                     /locus_tag="ACHKA1_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5681714.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            complement(5477..5863)
                     /locus_tag="ACHKA1_004142"
     CDS             complement(5477..5863)
                     /locus_tag="ACHKA1_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5681715.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAGEG
                     ELSEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAGDDEHEALTPEKS"
     gene            complement(5924..6667)
                     /locus_tag="ACHKA1_004143"
     CDS             complement(5924..6667)
                     /locus_tag="ACHKA1_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5681716.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEICAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASINVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDGDFVAVATVAVSAAG"
     gene            complement(6678..7079)
                     /locus_tag="ACHKA1_004144"
     CDS             complement(6678..7079)
                     /locus_tag="ACHKA1_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO5681717.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            complement(7076..7654)
                     /locus_tag="ACHKA1_004145"
     CDS             complement(7076..7654)
                     /locus_tag="ACHKA1_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5681718.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLTRR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLVNGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            complement(7666..7941)
                     /locus_tag="ACHKA1_004146"
     CDS             complement(7666..7941)
                     /locus_tag="ACHKA1_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO5681719.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQHWTVTRHERFNGKPMIFIE"
     gene            complement(7934..8257)
                     /locus_tag="ACHKA1_004147"
     CDS             complement(7934..8257)
                     /locus_tag="ACHKA1_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFO5681720.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGLTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            complement(8344..10371)
                     /locus_tag="ACHKA1_004148"
     CDS             complement(8344..10371)
                     /locus_tag="ACHKA1_004148"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO5681721.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            complement(10316..11824)
                     /locus_tag="ACHKA1_004149"
     CDS             complement(10316..11824)
                     /locus_tag="ACHKA1_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5681722.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFVQMVSGRINSLTP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKDYEDYELTAARIAAALGMYIRKG
                     DGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            complement(11824..12036)
                     /locus_tag="ACHKA1_004150"
     CDS             complement(11824..12036)
                     /locus_tag="ACHKA1_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681723.1"
                     /translation="MNQNDIEAMIQRYMEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            complement(12033..14135)
                     /locus_tag="ACHKA1_004151"
     CDS             complement(12033..14135)
                     /locus_tag="ACHKA1_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5681724.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSHSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            complement(14135..14629)
                     /locus_tag="ACHKA1_004152"
     CDS             complement(14135..14629)
                     /locus_tag="ACHKA1_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO5681725.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            complement(15182..15388)
                     /locus_tag="ACHKA1_004153"
     CDS             complement(15182..15388)
                     /locus_tag="ACHKA1_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681726.1"
                     /translation="MHKDQYTDAPSQEQVRVKTMLNSTISMGYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNTKDYSLVVD"
     gene            complement(15684..15857)
                     /gene="gnsB"
                     /locus_tag="ACHKA1_004154"
     CDS             complement(15684..15857)
                     /gene="gnsB"
                     /locus_tag="ACHKA1_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO5681727.1"
                     /translation="MNIEELKTKTEADISEYITKKIIELKKKTGKEVTSIQFTAREKM
                     TGLESYDIKIDLI"
     gene            complement(16030..16260)
                     /gene="ynfN"
                     /locus_tag="ACHKA1_004155"
     CDS             complement(16030..16260)
                     /gene="ynfN"
                     /locus_tag="ACHKA1_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFO5681728.1"
                     /translation="MKKIIVFFNSEPAMVVPVMTGVNTIMREYPNGETTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAMRLLN"
     gene            complement(16292..16330)
                     /locus_tag="ACHKA1_004156"
                     /pseudo
     CDS             complement(16292..16330)
                     /locus_tag="ACHKA1_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16333..16521)
                     /gene="ynfQ"
                     /locus_tag="ACHKA1_004157"
     CDS             complement(16333..16521)
                     /gene="ynfQ"
                     /locus_tag="ACHKA1_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO5681729.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDRNPFGAKCIFCKSMMITF
                     DNISQYLNVSRLSLDLRK"
     gene            complement(16532..16744)
                     /gene="cspI"
                     /locus_tag="ACHKA1_004158"
     CDS             complement(16532..16744)
                     /gene="cspI"
                     /locus_tag="ACHKA1_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HFO5681730.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSVIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT         3983 a         4983 c         3855 g         4199 t
ORIGIN      
        1 aggatcatac tggcccctgc tgcatacaga cccgatacag ccgcggcccc cagccagcca
       61 acagggttcc accatgccac cgcaatcagc gccgcaccca gcaccgcctg aaacacaccg
      121 ccacttttgg cacccgccag acgcggaacg acgtggatca cggcaccatt tgccagcggt
      181 tcattaagac gggcagataa ttcattttcg cctgcatcac gcccggaaat gcgcacctga
      241 taccagccct cattcagttt ctggcgaaac gacggaagct gtgtggccag cgcccggatg
      301 gcttcagccc ccgttttcac acgaaggtcg atgcggcggc caaatcgttg taaatccccg
      361 taaaggcaga tgcgtgccat gcccggtgac gccagaggga gtgtgtgcgt cgctgccatt
      421 tgtcggtata cctctctcgt ttgctcagtt gttcaggaat atggtgcaac agctcgccgt
      481 caccacagta aatggcggca tgattcggca ccgatgaacc aaaacagcac agcagcacat
      541 cgcccggctg cgccgctgac aacggcacct gatacagccc tgtggcctcc agattatcca
      601 gatagagatt ctgaccgtga cgccaccagt catcctcgcg atgaaaatcc ggcatctcaa
      661 tccccgccag atgataagca tcccggaaca gcgtgtaaca gtccgtcacc ccgtgctcaa
      721 agcgccgccc ggtgagatgc ggcacacagc ggaacttatg aatcgtcccc cggcagacca
      781 gccaccacgg caaatcactc tgcacctgca gccgccggtc ggcctcactc agccagggca
      841 gaccaccggg gtggctgtgg accagcgcca caatctcacc ctgcatttct gcctgcagcc
      901 agtcttccgg cgacatacgg aaatacgcct ccggctcacc ggagatattc acgcagggaa
      961 aatatctttc cccctccggc gctctcacca cgaagccgca cgactccgct ggcgcacatc
     1021 gccgggcgtg cgccagaatc gctgattctg tctctgtcat gggattactg cgaaagtttg
     1081 ttaatggaaa ggaagccgcc aaagttgccg acgttattgc gaaacttaca gccgctcagg
     1141 catttgctgc atttatcctt cgtgatatcg gacgtcggct ggtcatattc atccgcgacc
     1201 gccggaccgc tataaccgca ctcatcgccg cgataggtcc aggtgcaggt gttggccagc
     1261 atgatacgcc ccggaaaaac agagccatcc gtttcagtcg gtgtggacag tacaaaggag
     1321 gtactgaccg cactcagttc gctgcactgc tcgatgcgcc agcggctgat cacctcctgc
     1381 tccggatcgg cgtcgctgtt tccgttgacg aagttcaccg catccagaaa acgggcgtaa
     1441 accttacgcc tgaccaccgt tccgccgacc agactctgca ggtcttccgc catcccggtg
     1501 accatgccgt gcaggttaga aaccgtcagt gtcggacggg cagcactgcc cttgccattc
     1561 agttcaaatc ccgtcccctg aatggggtat gcctgatact gccgcccctg ccaggtaacc
     1621 ggctcacctt tttcgttctg ctcattacag aaaaaataac gttcaccacc gacctctgtc
     1681 agatcgattt cccagagcac cacctgggct gactgagtga ggcgtgtcgt ctcatgatgt
     1741 gtttcctgtg gaatatcctg catcagagcc tcctatgcca cgacctgttc aaaatctgcc
     1801 gttatggtta cccacagcgc ccccacgctt gccgaccatt tgcgacaaac caccctgatc
     1861 ggtttccagt cataaggtgg cgtccactga aatgcgcgga cgccaccgtg ccgttccaga
     1921 aaggctttta aagatgggtg ttcacattta cgaacacgta tcgtcacgct gtaagtcgac
     1981 aactggttat tcagtcccgc cgcacgacgc tgttcataac catcgcccag cttcactgtc
     2041 accactttcg gctctgatac cacattcata tccgggcgca ctttccagtg aaacgtctcc
     2101 attaccgata tgctccactt aaccgaccac catcacgggc ctgctgttgc ataaagtccg
     2161 ctgccgcttt tttcccaagg tcataaacca ccttcagggc agccggacct atctgcccgt
     2221 tcgtgccatc gttattgatc tcgatgttgt actgcggggc aaacatcgcc atacctgaac
     2281 caccaatatc cgccacaacc cccagctttc cgtcagcacc acgacgcaga ggcagaatgg
     2341 cttcaggtcc cgcttccccc atcacacccg cgccttttgc aaaagcaaaa aacgtcggac
     2401 ggttaaccac cgtgccactg taacgactca aatcagcaga ctgataaatc cccccattgg
     2461 cattcaggga aaggctgctg agatcaaatc ccagtacact gccaatccct tttatcgatt
     2521 tcatcatggt tgcctgcgcc aggattttcg ccatatctga cagcacagaa gaggtgaagg
     2581 atttgaaatt gagtttgccg gtagtgacaa aagttgccag gccatttccc atgtagctga
     2641 aggctgacga gaacacctgt tctgctgttc ctgccgtatt atctgcatcc acggtaaaat
     2701 cctgaaatgc acgcaggact ccgtttttcc agttaccctg cacaacttca agctgttgcc
     2761 agtaacggcg attctcattc agttgtcggt tcaggctctc cgtcagcgcc tgctcggcct
     2821 ttctgtagtc atccgtgtta tatgttcctt tctgctcact atcccgcctc aactgctcca
     2881 gctgttgctg gtatttctgg cgaagactca gttgtacctg atatcgctgc cgctgctgat
     2941 cacccatacc caccgtggca atatccaggt catgttgctg acgctgagcg cgctcttctt
     3001 cagccagttg actggtcagc tgaattgttt ttttcttcag ctcgttgagt gccgtctgtt
     3061 tctgaagctc ctgctgtttt acatccagca gcgtcagtgc ctgaatcagt tcatctttac
     3121 gggccagcac actcttttca tctgccgtca gttttttccc gtccaggtcg ctgatgcgct
     3181 gctgcagagc cagaagctgt ttatgagctt ctgtcatcct ttccgtggca atgcctgatg
     3241 actgtctggc agcagcaatc tgtccttcca cctgtgcctg ttgctgactg tactgcagca
     3301 ataaccgggt ggcctcatca ttacgggttt cgcgtgtttt tttcttaccg gatgccaggg
     3361 ctttctcgta acgttcattt tcacgttgta tcgccgcatc cctgacagcc tgatcggcgt
     3421 actgcatggc attaatacgc gcaatttcac gctgatgtcg tgctgcttcc gtttcattca
     3481 tccggttcag tgcagcattt tcagcattac ggcgtttctg ttgctcctga taattccgct
     3541 ctgcctgctc ttttgcatcc tgcaaatcct tctggcgttt tttctcctga agctcgttaa
     3601 gacgctgctg atcgtattca acctgagaag atgatgccgt ccaggggagt cttttcgccc
     3661 gcgacacttt ctcctgtaaa gcggaaatct gttcatccag cgagtcttca cgaccaatat
     3721 tcatggccgc atcccagaaa cgactccata aatcagacag atacttcagc gtactgccca
     3781 gcgcattgag gttattatca atatccgcag tacgccgacc ggtttcctct gccagtgcag
     3841 acatggctat ccgtgcagca tcactggacc gtccctgatc cccaaggaca cgtatctgct
     3901 caagctgagt ggcagtaaga aaatgcagct cattgtccag agccttcgcg gcatttacag
     3961 gatcatcctt cagccgctta aactgattta tggtatcgct gaccgactgg ccaaccgatc
     4021 gctccatctg tgcggcagct ctcgccacca taccgatatc gtttccacga aatgcaccac
     4081 tccccaccac ctgagccagc gcaccggctg cagcatgttg cgtgatacca ttcccggaaa
     4141 tagcacgact aagcgtccac agctgcccgg cagtgactcc ggcataatgc cccgtcagcg
     4201 acaactggcg gttaaattct tccccctcct tctgaccgtc ataccaggct ttacccagac
     4261 catagacggc cgcggcaata ccgccaataa ccccgcccag catcatgcct ttcggtgaca
     4321 tcagtgtgtc tatccatccg gcacggttag ccagcgttat cccggatccc ctcagcgcac
     4381 ctaaattgcc gcgagccagt tcacctatca gaacgcctat ctcctggcgg gccgctgcac
     4441 ttttcagacc cagcgaatgc gtggcttttc ctgcctgctc cattttgcgg atatacactt
     4501 ctgcagcact gcttaccccc agctgggcag ccttggcacg aagcaactca gaagaagaaa
     4561 gattctggcg ggttgcctgc tctttaagct gacggataaa cgccactttc tgtcgggtag
     4621 cctcttcctc agcctgtgta agaacacggg ttttcgccgt aacctcagaa atcagcgcca
     4681 gataatcctg ctgaccaatc ccgccactgt ttctggcctg tcggatctgc tgctgaatac
     4741 gctgtaattc ctgcagcccc gcactggcct gtttcacact gtcaatctga cgataaaacg
     4801 cagcagccgc tttatcctga gcctccgcca gagccatggc ctgcgcctgt tcatcgcgca
     4861 ttttctggct cagtgcctcc atgcgctggc gggttttctc cacctcgcgg gccatgcgtt
     4921 catgagcctg tgcgctcttc tccaccgtct gcgcatggac ggaggcggct gttgcagccg
     4981 aagaagccgc ctgcgttgtc tgccgggcgg cctgagtctg acgctccata aaacgctgca
     5041 tacgggcaga agaccgttct gcatcgctgg cggcaccatt cagaaggttt ttgatacggg
     5101 gaatttcatt tttaaactct gccgcatcaa tccccaaatc aatgaccagg ttggctatct
     5161 ggtccataac gcacacctcc ggaaatacct tctccaagat gcatcagttc ttcgtccgtt
     5221 cgctccggta tcccgttctc ttccggtaaa aggctgaaat cagccaccgc agcatcactg
     5281 ctgccggaca ccattctcac gatcaatgcc ttcagcgagg caaactgcgc atccatccac
     5341 acatcactga agctctgcat ccggaaataa tcgccccact caccaagctc agtggccgac
     5401 atttccgaca gcatccgccg ccagtctgcc cgccggaact cccgggcaag ccgcatgaca
     5461 aactgcattt cccgcgtcag gacttttccg gcgtcagcgc ctcatgctca tcatccccgg
     5521 cattatcaat ggcccccata ccgctcagcg acagaaccat ctccgccccc gcccccaggg
     5581 catcatacga ccatgttgta ataacggatg cgcaaagcgt ctctacatcc ttagactgat
     5641 ccgcattcca cagtgatcgg gaaaccagcc aggcattgat atccatcccc atccgcagaa
     5701 aagcaatctg tcgttcagcc tccgacagtt ctccctcccc ggcatcaaac tttgccgttc
     5761 gctgctgaac aaacgccaga tattcaattc tctgcagccc ggacagctca ctgagcacca
     5821 cggactgttt ttcataatta aacgtgccct gtttcagaaa catcatattc tccacctgca
     5881 aaaaagcccc gaataaccgg ggcaaatgat gagtatcgtc ctgttaacct gcggcgctga
     5941 cagccaccgt agccactgcc acaaaatcgc cgtcagaagt catgcccaca atgctgacac
     6001 tgccctgctt cacgcctttc accgtggcca caagtccgtt cagggtcaca gtggctgtct
     6061 gtggatctgt cgaatgcaca ctgatcgctt tgtcactggc tccgtcaggt tttactgtaa
     6121 aggtcagcgt ggtggttgct cccacattga tactggcgga tgccggtgcc accgtcagcc
     6181 cggtaacgcc cacggtttca gtgccctcct ctgccagata cggacgcccc acaccgctga
     6241 ttttcacggt gcgggtcatc acgtcttttg aggcaatggt tttacccagt gaactcagcc
     6301 agccacggaa aacatcaaca gtgccgttgg gatatttgat acgaaacgcg cagatttcac
     6361 cggagtcgaa caactgaacc agttttttct gtccgctgtc acccggacgc caggccagcg
     6421 tcgccgaagt atcaccgacg gatttctgcc cctgagttgt cgttttccag tctgcatctt
     6481 catcatcgag ataagtgtca tcttctgcat cagcggtcat ttcgccaggt tgcagatcct
     6541 tcaccatcgc aagacgcagc cagtcagtgt ccgataaagg gttcgcaaac gcatcgccct
     6601 tgccggtgta catccagaac gtcgttcccg cacctttcgt ttttgccagt ggatttggtg
     6661 tggtcattac cacctcctta attcgtgtac gtgatctggt acgtgatttc cgccatcgcc
     6721 caggtggcca tctcattatc acgttgatag ttaaaaccga gtgggatcag ggtgtcgatg
     6781 agtccggaaa gtgccggtac atcattcagg gccgggaaaa tggtgctctc catccacata
     6841 tccagctctg aatccggtgc ctgtgcccgg atgaagacag caatatgcag aacagcctgc
     6901 cagtcatctt catccgtcat ttttccggtg tactgagcat cactcagcca caccgccacg
     6961 gcaggcagtt cctgcgcatc aacaaatgcc ggaagtccgt caaaaaacgt ggcgctgtct
     7021 ccacactgtt cccgaaggcg tgccagtacg acctggcgga tttgtgtatg tcggttcatc
     7081 gggtcagcca taacctcagt tgttgtttca gtgcataccc cagctgtttc ggcatttccg
     7141 cagcaatgat gcggtcgcgg gcatcttcaa atgcctgtgt cagcggtccg gacagaggga
     7201 ttttcaccac atcaatgggg taacgatttt tgccatcaat acgccgcatc acatgccagc
     7261 gaccattcac cagttgctga ataaacgcat cccggaaaag atatttaccc accttcagca
     7321 cgctgccacg gtactgcagt tttccaccac gccgggtcag tctgacccgg gcggtcccca
     7381 gcttaatggc aggcagattg ccccggttaa cgcggatcct ggccgtcatt tttcctgacg
     7441 gactggcttt aaacacccgg acacgctgac gtaccagttt caggggtatc cctttcacct
     7501 ggttatctcc cgcaacggta ttcccggcaa cctgccgggt ggcaaccgag accgctttct
     7561 gtgccacacg gtttatcgcc catgcgctgg cctgtggcac catacgggta tcaaggctgt
     7621 tcagattgcg gatggcattc tcaagcccct tcatcccaca cctctttact caataaagat
     7681 cattggctta ccgttaaagc gttcatgccg tgtgaccgtc caatgttgtc cgtcataaac
     7741 aacgcgatcc ccgcgccgtg ggcggtatcc cgaagaaaac accaccagag agaccgcagg
     7801 tccggacaga gcattcagct ctgccagtgt ttctcccggg atcacagtca tatcgacatc
     7861 attaatcgag gctgtctttc ccatctttct gaccgtgatc gcatccatac gcgctgccag
     7921 ccgggaaaag ggatcagaca ttgagtttta ccggcacttc ttctgcactg gttccggcat
     7981 ctgcccagac aaccccgacc agcggatcag agccgctgtt agtcaactga acttttccgg
     8041 acttcagata aaccttctta cccgttttca tgtcatccgt tttcagctta ggcagcataa
     8101 acacaccttc ggtcaggccg tcgcctgttt caccctgtgg aatatcggtc agcgccaccg
     8161 caaaaacatc acccacctgc accaggtctc cgctgctgat ggctgcactg gcaacaatcg
     8221 ccaccgtttt tccttcttct acaaaattct ttgccataac tgtctccgca cagcctcgtt
     8281 caggggctga tttcaggtac aaaaaaagcc cttacgggcc atcagagttg ttgtctgcga
     8341 cgtttacgcc gtacatttca ccagaccgcg gtgatcaact ggcgcgacac cggcgtcaat
     8401 acgcactttc gttgtcacgc catccacact gaagccctcc atctgatcaa tatatggcgt
     8461 atccacaccg ttgagataag ccacttcaat cgtatcggag ccttttgacg cagccaggta
     8521 gaaggtggtc tggctgttat catcaagacg aggctctgca ataacggtcg caaaatcttt
     8581 caccgggtta ataataccgg cgttaatgtc agcccccttg acacttgagg agcgaatgac
     8641 ctggttagca acagactcca tcgccgtcgg taccagtacg aacgcaggac gaatattcag
     8701 atgacgctcc ccctctttct gaacgcgcat caactggcgg gctttatcca gcgatgccac
     8761 gtccattgca gcgctctcca gtacgtttgc atgtttcgct ttatcgaaca gacttacatt
     8821 atctgtggag attttcgggt tagacgtcag aatggcataa accagatcgg caatagtgga
     8881 tttcgccgca cggcccagtt tcatcgggac atcggtcagc atattcagat catcattgat
     8941 aatggcctga cgggtgatac tgaacagctc gccataggtc gccagtgcaa tagtggcctg
     9001 tttatctccg gtggtgacgt atttatattc cgccccttca cgcacctgac gcagagcact
     9061 gaagcccccc atacccacac gatgggcaat tttaaaatca gacaactgac ctttccgcgt
     9121 ccactgttca taggtttcag gggcatcttc ccagccctgc agaatggctt tgttcgcaac
     9181 atccagcaga atattaccga agtcagacgt actgtgtgtg aacgccgcac cgaccatttg
     9241 catcgggtta taactggaaa ccccaatacc ccgttcagtc agtgacatac gggcatattc
     9301 acgcagggtc atcccgttgt agacattatc acgttcggtt ttttcaaatc cggcacgcgc
     9361 catcagcgcc tggcggatcc cgtcccccac aaaattaccg tttccggcat aaatatgagc
     9421 cggggtattt ttattggatg gcgtggactc gcgccccatc tcgttcaaca gcttttcgcg
     9481 ggcctgctcc agcgaacatt caggatcggc aagacactga gcctgcagcg tctgataacg
     9541 cccgccaaac atggcaaaca gatcattaat accgtttaca cgcgcttttt gctctgccag
     9601 tacctgcgca cggatactgt tttcatccac cacgggtgct gctgcctgca ctggcgtccg
     9661 ggaggctgca ggttcatcat cctgtacgcg tggagcactg ttgcgtggcg gagtaatcat
     9721 gtttcgaatg gattccggca tctttttaaa ttcctctgta cgttttgact gaatacatgc
     9781 cattgcctta acggctggcg tcacctgatc agcaaatccc tgtgccagac attcggcacc
     9841 ggacatccag gtctcatccg ccagcatggc agcaatttca tcggtggttt tcccggtttt
     9901 ctgtgcataa gcgggtaaca gaaccgcctc aaccttatcg agcaggtcgg cataggtgcg
     9961 catgtcctcc gcatcaccgc ccgtaaagcc aaatggttta tgaatcatca tgaaggtgtt
    10021 ttccggcata atgaccgggt ttcccaccat cgcaatgacc gacgccattg acgccgccac
    10081 accgtcgaca taaacggtaa tggacgcacc atgtgttttc agcgcattaa aaatggcgat
    10141 gccttcaaag acatcgccac ccggtgaatt aatatggaga ttaatgtggg tgatatcgcc
    10201 cagtgcattc agatcactga taaactgctt cgctgtaaca ccccagaaac caatctcgtc
    10261 ataaatataa atatccgcgt cactctggtg accagcctgc atcctgaacc aggaattatt
    10321 cttcggactg gtcgtcggtg tgctgcggct cctgtcgttt cgttgcggca ctgctgcctc
    10381 ctttatcact ggccggatcg gtatcaaata ccagatccag cttgcggttt tcatcaattt
    10441 cggccttgcg ccgacgtttg acatcatccg gattacgacc accagcacgt acccagtctg
    10501 attctgtcgc cgctccacca cgaatctgga ttttccaggc ctcagcctcc ttaacagggt
    10561 caatccacgg catcactggt ccggaataca ccgcggtata cagtgaagaa cggtcaagat
    10621 cgcggggtag cctgataaca ccggatgcca cagcctgttt cagccatgca cgatacatcg
    10681 ggcgggtgac ggcaccaata aaccagtcct gcaggatcag gtagccatca gtagattcaa
    10741 ccagctcctg acgctgggcg ctgtaagtgc cgttatagtt gcgcgctgta ctggaaaaac
    10801 tcagacgact gcccgccgcc acggcacgca actgaccatt acgaaaagtt tcaaggttag
    10861 gattggggcg atccgacttc accattccga tttcttcgcc gggtttcaga tcgtcgtaaa
    10921 taatgcctgg ctgaatggta agctcgcgtt ccttatcctt gctgccatta ccatccggtt
    10981 catagctctg cccgtcgcct ttgcggatgt acatccccag agcagcggcg atccttgctg
    11041 cagtcagctc ataatcttca taatctttca gggcactgag gcggatcagc acaccggaca
    11101 acaaagacgt cccgcgcatc tggtgcagac ggcgaacaaa tttaagatgc agcattcgct
    11161 ctgcatccac ttctttggtt tccatctgcc gtccggatac gggacggctt ttatacacca
    11221 gatatttttc gggacgcccc cagtcatcaa caaacacgcc ctgattcagc ctgttgctct
    11281 catcactggt catgggaata aagtctggct cgagcgcctc cagccagaaa tgaacaccgg
    11341 cagaaggcgt caggctgttt atgcgcccgg aaaccatctg gacaaacacc tcaccatcgc
    11401 gcagccaggt acgcagcatc agacgttcca gcatcggacg ggtaaactgc ccggtgactt
    11461 ccgggctgac agaccattca ctccatcggg tgcgaatctc cgctgccaga tcacgggcaa
    11521 tggccccatt gcgtaatacc ggatgtggct cgacaataat cccgtttttc cccaccaccc
    11581 gttcttccag cttgtcaaat acaccaataa ccagatcgtg gttgttatca aggtaacggg
    11641 cctgctcacg taacgacacg gccccgtact ggcttagctg gtcggcagtt cggttctccc
    11701 gtcgggcttt gtgtgtccgc gtcgttttta cggcttcata agcctggatc accgcacggg
    11761 aacgcagcct tgccgctttc catccaggtg aaaaaacgcc aatcacatca tcaagaattg
    11821 ccatcagaac ctcgccagcc ggtacccggg atgcccccgt cgtcgtgtaa tcagagccgc
    11881 aaggcggcgc tcccactcct gccgtccctg ccggatctca gataagtttt ccatggtcat
    11941 ctgctgacca ttaaaggtga cggattttcc gtccagcacc gccatttcag cttccatata
    12001 acgctgaatc atggcttcga tatcattctg gttcataacc atcctccgga agtcagccag
    12061 gggttaacat cgtcagttac tgttttcttc cgtttttgtt ttttaacagg cgtggatacc
    12121 ggttccggtg aggatgacgg ttcggtactg tcctggacac actccagcca ggtttcccgg
    12181 ctcgcccact ccggtgcatc cggccagcgg atcttttcgt atccatgcag aatgaccaga
    12241 gcctcggcat acaccatcag gtcaaaagct tcgttggcac cgcgaccagg cttactccat
    12301 ttcccgtcac tgctccgctc ttcatacgtc agttcgtcgt aaaaccagct ccccagccag
    12361 tcagggaaat gcacatagcc gggacctggc gagtcacgcc ataacgcgtt attcacccgg
    12421 tctttcagtg catccgtctg aagaagccag agcggcacat cacctgcggc ctgcgcccgt
    12481 cggcccgttc gtccggtgtt atcagggaat gtacgagtga tcagttttgc gcgccggatg
    12541 ctgtcgccct taaacaggta aatacgttta ccaaggccat cacgacggca acgacgccag
    12601 aatttatagg cattatcagt gaccccgtct tcaccgccgg agtccaccgc cattgccatc
    12661 agtcgcattt gttgagaagg atcggaggcc agcggccagc ttttatgaaa aacatccgtc
    12721 agcaggacat cccagtcttc cggatagctg gccggatcaa ttcgctggct ctccccgtcg
    12781 ctgtcaccgc gcaatgactg cgtgatgttg taacgatcaa taatccagcg ttcgccacgg
    12841 ctgccatagc ccgttacctg aaccacaaaa cggcgatgac gtcccgcctg cacatccact
    12901 gtcgccacaa ggaaattaac gccatccggc acactgtggg aaggaactgg ctctgcccgc
    12961 tgctcaagca gttcactttt tcgttgctcc atgctggcgc ggggaagata aggtaatccc
    13021 cagtcggtat tgataaccgt cttgagtgtt tcttcacttc cggttgtctc gtattcctgt
    13081 tctgcagtaa gcagtttgta aacgagttgc gagagtgtct ggtaagcagc tgccggaccc
    13141 tccatccaga atgacgcaat acgtgagcgt cggggatcac cataacgact gccatccgca
    13201 ttgatggatt caccatcccg caaccagacc ccacgtccgt tcagctcacg tttttgttca
    13261 ggcataatcc gtcctgaaca ggaaggacac tgaatataag ccgcctcact tgccagcacg
    13321 ggatcggcaa tatcacggaa accagcaacc acatcgccgc agggctgaaa atactcacca
    13381 cagtgtggac agggccagta ccagcgacgg cgatcgccac ggttatagag cgacagtatc
    13441 cccgtggttg gtggagcctc atgcggtgaa gtccgtcgcc atttcacatc cttcacatcc
    13501 ctgccggggg aactctccac aagcgtcata ccactggaca taaatgttgt ggtacgtttt
    13561 gaggcaagag agaaagcatc cccctcgcca tcaatatctt ccggaaaacg gtcataatcc
    13621 gtcagcgcga cgcatttata atctgatgag gacatgatat tgactgacgg ccagccgatt
    13681 ttcaggtagt taccagcaag gaatgttctg tcataaacgt tgttgtcatt tttgttcgga
    13741 ctcaggcgac tgaccacttc cgggctgacg cgaaacgttc tggcgagtcg ttttttggag
    13801 tgttcgcggg ctttttcctc cgtcatctga atgatcagca tatcagcagg atcgcaaatc
    13861 acgttgtaaa tcacccagcc gtcaatcagg ccgatagtct tgccggttcg tgccgggcca
    13921 acaaatatca ctgcgtcgta ttcacgcgag gccaggcagt tcatcggctc aataacatac
    13981 ggtgccacca gcggatccca cgggactgag ttccctgccc ccatgggcac ccgcatatac
    14041 tgagcaacgg catcagcaac ccgcattcgt ctcggtgcgc gaaggatata acctgaatcg
    14101 gttcgtgctg cctttgcggt ttcctgattc agcattactc ctcctgctgt aattcctcct
    14161 catcatccgc acctgcttca gtcacccgca gggctatctg atcgcgcaga tcatcaataa
    14221 tggactgaac acggctcaca gcggcaggct gcagaccgca gtcacgttca agaatatccg
    14281 gtaatgtctc cagcacctgc acgaccgctt ttgcccagat ggcaaactcc cgtctgacat
    14341 cactggccgg aatgagttgt gccgtttcct gttcgaactt aagacgctca cgttcagact
    14401 gataccaggc tttgcgctca tgcgcgtcca tttcgccttc tgcaaccggc ggtggtaatg
    14461 ccagaaatgc cgacacaata tcaaccaccc gataaagctt gaggttgctt tcatgccccc
    14521 ctgcaacggg tagattttgc agccttgccg cagcagtctg gcgatgtaca cctgacagtg
    14581 ccgccagttg actgatattc agcgtcagat tttttaactc tcgatccata cccgctccag
    14641 aatgttttaa acatgcatct tgcgaacaac tttaggcaaa cggtgttagt gatgaacaaa
    14701 aaacaatcaa aatcgacacc ataaaaataa aaccactgta atatcaatct attacagtgg
    14761 tggtgatgac gaatgaaatt tcaaaaacta gcctttttcc gcgacgctcc cgccccgtgg
    14821 cagggccccc cacagggagg acccgacagc ctgacaaccg tgacgagcat ctgaaacaga
    14881 gctttacata atggcgtggg gataattcag aaggacatca cagcatgccc acacaaatta
    14941 gtgtgagtgt cctgtttctt actacgcgca ggactgacga gcatgagggg aaaatacgcg
    15001 aaccgaaatg ccttatcttc agaaatgcag ccggcatccg aactggacga tattgtaaat
    15061 tcacctaaat acgaggcatt gctgtagttg aattgcagta ctgtattagc ctgacagtga
    15121 cagaatacga tattgactct gtcacttgtg aaatggtcag aatggttagc agttatggtg
    15181 atcagtcaac caccagggaa taatccttcg tattcttatc gtgctttact aacgcggcct
    15241 caattgccct gaatgcttcc agagatactt gatgttctat acatgcaatt acaacatccg
    15301 ggtaacccat cgaaatggtg ctattaagca tagtttttac acgaacctgc tcctgggagg
    15361 gggcatcagt gtattgatct ttatgcattt tgtcgctcca tgcgtttgct cttcacttgc
    15421 ggctaaaaat ataacgtcaa atcactcagc atgaaagagt gaaagtttca gcgcgtttcc
    15481 cttacataat ctgtcgtttg tattctcttc aggcataccg gaaagactag gtattgtcac
    15541 acaaccaata ccatcgacaa tcgagattta tatctattcg gtaatgttta aatataacaa
    15601 aaccccgtaa aaacgaggtt tatgggtaat ttgtattgtt gaatagcatc tgatgagaaa
    15661 ttgatgctaa tactataaac atactagatt aaatcaatct tgatatcata gctttcaaga
    15721 ccagtcattt tttcccgtgc agtaaactgg atactggtaa cttctttccc ggtctttttc
    15781 ttaagctcaa tgattttttt tgttatatat tcagaaatat ctgcttctgt ttttgttttt
    15841 aactcttcaa tgttcatcat ttcctctttt agtctgttat gactttcctg gtacacagta
    15901 gtgtcaatta tatggagcaa acgtataaaa gataagatga aacatcgcaa taatcaacat
    15961 acgacggtct aaatttcaca caaacagata aagatgatta tcatttatta tcaatgcatt
    16021 agaatcaaat caattcaaga gtctcattgc tgcttccaga atttcttctg aagttacatg
    16081 tcgatccgct gctacataaa tgactttatg atctccggtc agagatggaa accctgcagc
    16141 cattacagtg aggtgtgttg tttcgccatt tggatattca cgcatgatgg tgttaactcc
    16201 ggtcatcact ggcactacca ttgctggttc agagttaaaa aaaactatta tttttttcat
    16261 aatgttaccg taatatgtga gtatccatcg actagacact aagcaaaaaa gctcccgaag
    16321 gagccttcat tttcactttc ttaaatctaa cgacagacgg ctaacattta agtattgtga
    16381 aatattatca aatgtaatca tcattgattt acaaaagata cattttgccc cgaaaggatt
    16441 cctgtcagaa acatcaaaag atgatgttct atactgagaa ccatggcaac acgggcatct
    16501 aaagtgaata tggtttgtaa tattttctac ctcaaagtgc cactacatga acagccgcgg
    16561 ggcctttagg tccgttctca ataccaaatt cgacttcctg attctcagtt aacgttttga
    16621 aatcgttgct ctgaataact gagaaatgga caaacacatc tttgctacca tctttcggcg
    16681 tgatgaaacc aaaacctttt tcagggttaa accatttcac taaaccagtc attttgttag
    16741 acatcattat taccttttga agaagttagc ccttgggcag aatggtccga aaaaaaatat
    16801 cagagagaaa aaccaacaag gagatctcaa gaggtacaaa taataaaatt ataacaatga
    16861 ctgcttcaga taattttgta acaaaccaga acaccattaa cgcatgatta accaaccata
    16921 gcaaggatta cttttgtaaa gaaaaacaca cgaatgaaac aatagcttta tttattaata
    16981 aaacgtgtca ttctgtctaa tgacctttta tcttaccctt
//
LOCUS       DAZFKF010000105        16670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFKF010000105 DAZFKF010000000
VERSION     DAZFKF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.105"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(74..226)
                     /locus_tag="ACHKA1_004159"
     CDS             complement(74..226)
                     /locus_tag="ACHKA1_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5681731.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(491..1225)
                     /gene="cysH"
                     /locus_tag="ACHKA1_004160"
     CDS             complement(491..1225)
                     /gene="cysH"
                     /locus_tag="ACHKA1_004160"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO5681732.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1299..3011)
                     /gene="cysI"
                     /locus_tag="ACHKA1_004161"
     CDS             complement(1299..3011)
                     /gene="cysI"
                     /locus_tag="ACHKA1_004161"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO5681733.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3011..4810)
                     /gene="cysJ"
                     /locus_tag="ACHKA1_004162"
     CDS             complement(3011..4810)
                     /gene="cysJ"
                     /locus_tag="ACHKA1_004162"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO5681734.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAEKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGVVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVESEVHVTVGVVRYDIEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5129..5491
                     /gene="queD"
                     /locus_tag="ACHKA1_004163"
     CDS             5129..5491
                     /gene="queD"
                     /locus_tag="ACHKA1_004163"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO5681735.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5569..6840
                     /gene="ygcN"
                     /locus_tag="ACHKA1_004164"
     CDS             5569..6840
                     /gene="ygcN"
                     /locus_tag="ACHKA1_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5681736.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDFPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTYPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHCE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYWAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6831..7091
                     /gene="ygcO"
                     /locus_tag="ACHKA1_004165"
     CDS             6831..7091
                     /gene="ygcO"
                     /locus_tag="ACHKA1_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO5681737.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQTAERLINACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7108..7683
                     /gene="ygcP"
                     /locus_tag="ACHKA1_004166"
     CDS             7108..7683
                     /gene="ygcP"
                     /locus_tag="ACHKA1_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO5681738.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7831..8691)
                     /gene="ygcQ"
                     /locus_tag="ACHKA1_004167"
     CDS             complement(7831..8691)
                     /gene="ygcQ"
                     /locus_tag="ACHKA1_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO5681739.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTISVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIDADCQ"
     gene            complement(8688..9467)
                     /gene="ygcR"
                     /locus_tag="ACHKA1_004168"
     CDS             complement(8688..9467)
                     /gene="ygcR"
                     /locus_tag="ACHKA1_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO5681740.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAAELRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCRQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9445..10854)
                     /gene="ygcS"
                     /locus_tag="ACHKA1_004169"
     CDS             complement(9445..10854)
                     /gene="ygcS"
                     /locus_tag="ACHKA1_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5681741.1"
                     /translation="MMGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGVLLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLILAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10876..12330)
                     /gene="ygcU"
                     /locus_tag="ACHKA1_004170"
     CDS             complement(10876..12330)
                     /gene="ygcU"
                     /locus_tag="ACHKA1_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO5681742.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVNCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12400..13185)
                     /gene="ygcW"
                     /locus_tag="ACHKA1_004171"
     CDS             complement(12400..13185)
                     /gene="ygcW"
                     /locus_tag="ACHKA1_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5681743.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            13504..14781
                     /gene="yqcE"
                     /locus_tag="ACHKA1_004172"
     CDS             13504..14781
                     /gene="yqcE"
                     /locus_tag="ACHKA1_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO5681744.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVLQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADIAPTTANSK"
     gene            14808..16286
                     /gene="ygcE"
                     /locus_tag="ACHKA1_004173"
     CDS             14808..16286
                     /gene="ygcE"
                     /locus_tag="ACHKA1_004173"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO5681745.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLMHRLTGEFKDNIANYFGQWPVDYKTWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     NAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         3974 a         4390 c         4177 g         4129 t
ORIGIN      
        1 ataaatttta aaaagggtgc aggagaccaa accatcacaa ttcagtacgc ggggttttac
       61 ccccgcgtgg tggctacttc ttcgattcgt aagcgagaac tgccttaaac tccataccac
      121 gttctctgat actcaactca cacaacgagt cgcctaccat cagcgtgaat cctagtactg
      181 tgcaacacag tacgatgatt gccacaaggg catattttgt cagcatgtct tgcctccctg
      241 caaagaagag actaacattc gaattgtttg ggttcgaacg taagcctcag gttgatagaa
      301 atatcgcctg gggcttttgt ccatctggaa cctcgcgaat gcttaacgcc agacagcctc
      361 aagcacccga cgccattcta tacctgataa ttcttccagc ggcgttcacg tttaaaatca
      421 ggaatgctgc tcgcaaaata aaaacgcccc catcatttct gacagaggcg tttaatttat
      481 ccggcaatat ttacccttcg tgtaacccac attccctttt taagccaaag aaacgcgttt
      541 cttcttccgc catgccgggt tcccatttac gggttgtatg ggtatcgccc accgataaat
      601 atccttcgtc ccataatggg tgatatttca ggccatgctt ttgcagatac tgatatatcg
      661 ttcggttatc ccagtcgata atcggcagca ctttaaatac gccacgctga attgccagca
      721 ccggtaaatt ggcgcggctg ccggattgtt cgcggcgcag gccagcaaac cacgtttgcg
      781 cgttaagttc tttcagcgcc cggttcatcg gttcgacttt gttgatgtca ttgtactttt
      841 caatgccttc aacgccctgt tcccacagtt ttccgtagcg tgcttcctgc caggctgcgc
      901 tttcggtagc acggtacact ttcaggttga gcttgagttt gtccgttaac tcgtcaataa
      961 agcggtaggt ttccgggaac agataacccg tatcggtgag gatcaccggg atatccgggc
     1021 gaatctgatt caccagatgc aggctgaccg ccgcctgaat gccaaagctg gaagaaagca
     1081 catattcacc gggcagatta tccagcgccc aggctacgcg gccttcagcg tccagttttt
     1141 ccagttcggc gttagtttct gccagcgcca gaatgcgatc taccttcggc agttcgttca
     1201 gggcgtttag atcgagtttg gacatagatt cctcactgtt tgccttgcct gatgcgacgc
     1261 tcacgcgtct tatcaggcct acaagaccgg gctgatggtt aatcccacaa atcgcgcgcc
     1321 ggatcgagca ccgggcgaat gatgcccgca cgcaccgtaa agtcgccgaa gccttcaccc
     1381 gcttcgcgct ctttcgccca gcgccctatc agttcatcaa gcgacgccag gatttccggc
     1441 tcggtgatgt tttctttata catccgtggg atacgtgtcc caatacggtt gccgccaaga
     1501 tgcaggttgt agcgacccgg cgctttaccc accaggccca cttccgccag catcgcgcga
     1561 ccacaaccgt tcgggcagcc tgttacacgc atcacgatat gctcatcgct gacaccatgt
     1621 ttcgccatta aattatcgat gttgtcgata aaagacggca ggaaacgctc cgcttccgcc
     1681 atcgccagcg ggcaagtcgg gaacgagacg caggccattg agttttcacg ctgcggcgtg
     1741 acggcattca ttaacccgct ctctttggcg atcttctcga tcttcgcttt ctcgctttcc
     1801 ggcacaccgg cgatgatcag attctggttc gccgtaatgc ggaaatcacc tttgtggatc
     1861 ttcgcgatct ccagcaggcc ggttttcagc ggacgccccg gataatcaag gatgcgacca
     1921 ttttcgataa acagcgtcag gtgccagtta tcatcaatgc ccttaaccca gccaatacga
     1981 tctccgcgtc cggtaaactc atacgggcga atcggttcaa atttgatccc cgcgcgacgc
     2041 tccacttccg ctttaaacgt ctcaaccccc acgcgctcca gcgtgtattt ggttttggca
     2101 tttttacgat cggttcggtt accccagtca cgctgagttg tcacgacggc ttccgccacc
     2161 gccagcgtat gctccagcgg cagatagcca aactcactcg ccgtgcgggc gtaggttttc
     2221 ttgttgccgt gttcgataga aagcccaccg cccaccaaca ggttaaagcc caccagcttg
     2281 ccgttttcgg cgatcgccac gaagttcatg tcgttggcgt gcagatcgat atcgttctgt
     2341 ggcgggatca ctaccgtggt tttgaattta cgcggcaggt aggtctggcc gaggatcggt
     2401 tcttcatcag tggtggcgac tttttcctga tcgagccaga tctccgcata cgcgcgggta
     2461 cgcggcagca gatgttcaga gattttcttc gcccactcgt acgcttctgc gtgcagctgc
     2521 gactcgtaag ggttcgaggt gcagagtacg ttacggttca tgtcgttagc ggtcgccagc
     2581 gcatcaagac cgaccgagtg cagcatctgg tgcaccggtt tgacgttctt tttcagaatg
     2641 ccgtggaact ggaatgtctg gcggttggtc aggcgaatgc tgccatagat ggtgttttca
     2701 ccggcaaatt tatcgatcgc ctgccactgt ttggtggtga taactccacc cggcagacgg
     2761 cagcgcaaca gcatcgcgtg gcgcggctcc agcttctgtt cagcacgttc ggcgcggatg
     2821 tcgcggtcat cctgctgata catgccgtgg aagcggatca gcaggaagtt atcgccctta
     2881 aagccgccgg tcagaccgtc gtttaaatct tccgcaatgg tgccgcgcag gtagttgctt
     2941 tcaagcttca tgcgctcggc gtctgtcagt tttccttcga ccactaaagg ccctggatgt
     3001 ttttcgctca ttagtagaca tctcgctgat aacggcgctc tacgcgcagc tcacttaaaa
     3061 attcatccgc cgcttcggtg tccatgccac caaattcggc aatcacttcc agaagtgcct
     3121 gctcaacgtc tttcgccatg cgattagcgt cgccgcagac ataaatgtgg gcaccgtcat
     3181 tgatccagcg ccacagctcc gcgccctgtt cgcgcagttt gtcttgtacg taaacttttt
     3241 ctttttgatc gcgcgaccag gcgagatcga tccgcgtcag cacaccctct ttgacgtagc
     3301 gctgccactc cacctgatac aggaagtctt ccgtaaaatg cggattgcca aagaacagcc
     3361 agtttttacc tggcgcttcg tcagcggcgc gttgctgcat aaaggcgcgg aacggcgcaa
     3421 taccggtgcc tgggcctatc ataatcaccg gggtttccgg gttagcgggc agacggaagt
     3481 tgtcgttatg ttcgataaag acgcggactt caccctcttc ttccacgcgg tccgcgagga
     3541 agctggaggc accacccgca cgggcgcggc cttcaatgtc ataacgaacg acaccaacgg
     3601 tgacgtgcac ttcactttcc acttcagcct gcgatgaggc gatggaatag aggcgcggcg
     3661 tcagcgggcg cagcaggtta atcagcgctt cagcatcaag ctgtgccggg gagaaacgca
     3721 ccatgtcaac aatcggcgtc gtcgcggcgt aatgctgtaa cttcgcttta tcgcccacca
     3781 gcggcagcag tgtttcactg cgggtaagcg tggcgtaatt ctcaacaatg ttggcggtgt
     3841 tgacggtcag ttcgaagtgc cactgtagcg cttcgttcag aggcaacgtt ttgccctcga
     3901 cggtgacagg ttcatcgcct ttcagccaca gcagttcgac aagttctttc accagtgccg
     3961 gatcgttctg ataccagaca cccagcgcgt cacccggctg gtaacgcagg cccgagtcac
     4021 ctaagtcaat ttcgatatgg cgaacgtctt tttcagagtt acgcccggta attttctggt
     4081 taaccgaaag gctcgctacc agcggcgcgt ctttgctgta cgggctggtg tggatttcat
     4141 ttaccacgcc agtagcgacg gattgcgaag gtgccgcgac aggcgcacgc gatttaagcg
     4201 catcaaccac gcgggcgcgc cactcgctgg cagcagcctg gtattcaacg tcggcgtcga
     4261 cacggtcgag caggcgttca ccgcccagtt ccgccagctt gctgtcgaaa tctttcccgg
     4321 actggcagaa aaattcataa gaactatcgc cgaggctaaa cacggcaaaa gcggtatttt
     4381 ccagcttcgg cgcttttttg gagaacagga acttatgcag cgcgacggct tcttccggcg
     4441 gttccccttc cccttgcgtt gacgtcacta cgatgagcag tttttcgctg gcgatttgtt
     4501 tgaatttata gtcgcccgcg ttcaccagct taacgttcag tttttctgct aataaatcat
     4561 cgcgtaatgc ttcagcaacc cggcgcgcat tgccggtttg cgaagcagag ataatagtta
     4621 tacccggcat ttctgcggct ggcgctggcg tcgctgcaag cgcagcaggc tgctgattga
     4681 gcacgcccca gaaatagcca gaaacccagg caagctgggt gggagttaaa tcggtcgtgg
     4741 ccgcctgaag gcgtgccagt tgctccgggt tcaacggaag caacgcggaa ggtgggacct
     4801 gtgtcgtcat gcgtcgttat gttccagtaa gcaaagctgt ttctgcgccc tgtcagcgcc
     4861 cataaaacag aagagaaggt aaggttaacg gggtgaacgg tgtggattaa agacgggata
     4921 gcgataacta ataaccaaat cgactaacct gttttagcaa tagtctttaa caacaaaata
     4981 gattaaacaa cctaatgaaa aacaaatgaa tttagccaat cattaagata aatcagcgat
     5041 tttgcgcaac aaagtcgttt tagataatgc gaaaaaacag cctttccggt actctacggc
     5101 ggttttatcg tcctgtagag aaattatgat gtccaccacg ttatttaaag atttcacctt
     5161 cgaagccgct caccgcttac cacacgtccc ggaagggcat aaatgtggtc gcctgcacgg
     5221 gcattccttt atggtgcgac tggaaattac cggggaagtc gatccgcata cgggctggat
     5281 tatcgatttc gctgaactga aagcggcgtt taagccaact tatgagcgcc tcgatcacca
     5341 ttatctcaat gatattccag gtctggaaaa cccgaccagc gaagttttag caaaatggat
     5401 ttgggatcag gttaaacccg ttgtgccgct gttaagtgcg gtgatggtaa aagaaacctg
     5461 caccgctggt tgtatctatc gcggcgaatg ataagagtgt gtcggcggtc aatttccctt
     5521 aagtaacgct atgttagggt gttgtgttca ggatatctgg ggcatgacat ggaagacgac
     5581 tgcgacatta ttattattgg tgccggtatt gcaggcaccg cttgcgcgtt acgctgcgcg
     5641 cgagcgggtt tatccgtttt gttactggaa cgcgctgaaa tccccggcag caaaaatctt
     5701 tccggcgggc ggttatatac ccatgcactc gcggaactcc tccctcagtt tcatctgacc
     5761 gcgcctcttg aacgacgcat cactcacgaa agcctttccc tgttaacgcc ggatggcgca
     5821 acgacgtttt ccagcttaca gcccggcggt gaatcctgga gtgtattacg tgcacggttc
     5881 gatccgtggc tggttgccga agccgaaaaa gaaggtgtcg aatgtatccc cggagcgacg
     5941 gtggatgcac tgtatgaaga aaatggcaga gtgtgtggtg tcatttgtgg tgacgatatt
     6001 ctccgcgccc gttatgtggt gctggcagaa ggtgccaaca gcgtcctggc tgaacgtcac
     6061 ggattagtga ctcgtcctgc tggcgaagcg atggcgttgg ggatcaaaga agtgctgtcg
     6121 ctggaaacat ccgctattga agaacgtttt catctggaga ataacgaagg cgcagcgttg
     6181 ctgttcagcg gcggaatctg tgatgacttt cccggcggcg catttcttta tactaatcaa
     6241 caaacgctct cgttagggat tgtttgcccg ctctcttccc ttacgcaaag tcgtgttccg
     6301 gcaagcgagc tgctggctcg ctttaaaacg tatccggcag tgcgcccgct tatcaaaaac
     6361 acggaatcac tggagtatgg tgcgcatctg gtgccagaag gtggcttgca cagtatgcca
     6421 gtgcaatacg ccggtaacgg ctggctgctg gtgggcgatg cgttacgcag ttgcgtcaat
     6481 accggaatct ccgtgcgcgg catggatatg gcgctgactg gcgcacaggc ggcggcacaa
     6541 acgctgataa gcgcctgcca gcactgcgag ccgcaaaatc tgtttccgct ttatcatcac
     6601 aacgtcgagc gcagcctgct gtgggatgtt ctacagcgtt atcagcatgt tccggcgctt
     6661 ttgcaacgcc ctggctggta ttgggcgtgg cctgcgttaa tgcaggatat ttcccgcgat
     6721 ttatgggatc agggtgataa acctgttcca ccgctgcgcc agttattctg gcgtcattta
     6781 cgtcgtcacg gcctgtggca tctggcgggc gacgttatca ggagtctgcg atgtctgtag
     6841 cccgtaatct ctggcgcgct gctgatgcgc cacatattgt tccggctgac tccgttgagc
     6901 gccagacggc agaacggttg attaacgcct gtccggcagg tcttttttcg ctcacaccgg
     6961 aaggtgactt acgtattgac tatcgcagtt gcctggagtg tggcacctgc cgtttgctgt
     7021 gcgacgaatc aacactacaa cagtggcgct atccgccttc cggattcggc atcacctacc
     7081 gttttggata aataataagg ataatttatg cccctcttac acctgctccg ccagaatccg
     7141 gtgattgctg ccgttaaaga taatgccagc ctgcaactgg caatcgactc tgaatgccag
     7201 tttatttccg tgttgtacgg caatatctgc accatcagta atatcgttaa aaagattaag
     7261 aacgccggga aatatgcttt tattcatgtt gatttgctgg aaggcgcgtc aaataaagaa
     7321 gtcgtgattc agtttttgaa actggtgacc gaggcggacg gcattatcag taccaaagcc
     7381 tcaatgttga aagcggcaag agcggaaggt tttttctgta ttcatcgcct gtttattgtt
     7441 gattcgattt cgtttcacaa cattgataag caagttgcgc aatcgaatcc ggattgtatt
     7501 gagatcctgc caggctgcat gcccaaagtg ctgggctggg tgacggaaaa aatccgccaa
     7561 ccgctgattg ctggcgggct ggtatgcgat gaagaagatg cgcgtaatgc gattaacgcg
     7621 ggtgtcgtgg cgctttccac cacgaatacc ggagtctgga cgttagcgaa aaaattgctt
     7681 tgacgggata atcgtaacca attgaatttg gtttgatttg taggccgcac gccacatccg
     7741 acattcagcg cctgatgcga cgcttaacgc gtcttatcag gcctacaggt ccccagtcca
     7801 gaaccgtagg caggatcatc cggcaacacg ttattggcaa tctgcgtcaa tgtttgtcac
     7861 cagtgcctcc agcaccactt tccagtcatc aaccacgcca acatctgcct gtgaaaatac
     7921 cgctgcgctg gcgtcatgat taatcgccac cacaaacttg ctattgcgta cgcccgccat
     7981 taacgctgct gcgccagaag cgccaacaac aatacacacc tcaggtgcca gcagatgtcc
     8041 ggaaatacca atcacttttt cagcatcaac gccaccgttc atcactcgtg cccgactgta
     8101 gcctacttcg gctcccagct tttctgccag catagcaatt tcctggttat ctgcctctcc
     8161 gccctgcccc accaccagca cccgtctggc ctcagctaat gggtcgcgag taacattttt
     8221 cagatcctct gtgctaacca gccagtccgg aagcgcaccc gggacaatgt tcagttgttg
     8281 cattccagag ggcaacgtgg cgtttttcgc tgctcccgcc tgacgcgcca gggaaaggca
     8341 tagcgggcgt ttttctgttt gcagcgtcgc cgttagcgca tttccccagt gggatttgcg
     8401 cacgcttatg gttggaatat ctaacgaagt cacctggcag atgcttgcac catgtaaacg
     8461 ccaggccagc cgggtcgaca gttcatcgcc aaacgttccc ggcggaaaga gaactacgtc
     8521 cgcaggcgtt cgctgccact gttccaccaa cgcatccagc acctgttccg ccactacagg
     8581 ctgaggttca atctgccagt gcgccagggt acaaccgctg aaatcctgtg ccgccagcca
     8641 gcttgctatt gcggcattct cctgatttat tgtcacaatt gcgatattca tggctgcatc
     8701 ctttggcgta aatagtcctg ccatagcttt tgcgcttttt ctgctaccgt ttgcccatca
     8761 atcagcgtcg ctccacgtcg ttgctctgct cttgccagtt gccgacactg catcgctggc
     8821 atttcggcag caacagtttt acggataatt tctgctttcc ccgcagccat tcgctggcgc
     8881 attcccggta ctggcaaggc cacttcaccg cactgacgca cggcaatcac cgcaggcaag
     8941 cgaacccggc aacaacgcaa cccatgttca gtacgctgtt cgagggtgat aaacagcgcg
     9001 tcgagagtga aacgctccac ctgggtgaag cagggccagc ccagcatttc cgccagcaaa
     9061 aatggcgttt gcccattctg cccttcgctg ctttggcagc cggtaattat cagatccagc
     9121 ggattctgac gctgccattc ggcaatatgg cgagcgacaa attccggtgc aaagcgcagt
     9181 tctgccgccg tctccagcaa tacagcttcc tcaaacccaa gagccatgag atagcgcagc
     9241 cagtgcaacg cccgttcatc ccccatgctc aacgcggtta aagacatcgg cgtaccgttt
     9301 tttctctgcg ccaacagcag cgcggcggca gcctgttcat cagcaccgag taaacttcgc
     9361 agtagcgaaa catccggtcc gctattaccc tgagccgccg cctgccactc tttttccgcc
     9421 agcattccgg catccggttc ggctttaaac gctaacagaa tgttcattcg cacctcctac
     9481 atttcccgcc gccaccagcg ggagtgcttt ggtttctggt gcccatagcc aggtcacgac
     9541 aaaaccaact aacaacactg ccgccagaat caataaggta acctgcattc cccactgcgc
     9601 cagcacccac ggcagcaggc cagtacttac cgccgcgcca agtcgactca tggcggtggc
     9661 aaaaccgacg cccagcgagc gaatgtcagt aggaaaactt tccgcaggca aaatgcccac
     9721 cagattactg actgccgaaa tggtggtgct gaagagaaca aaaagcagca gcgttaatga
     9781 actgccggaa ggcaaacagg ccattactac cagcgttgcc gccagcagca aaaaacttcc
     9841 cagcaaaaat ttgcgatgtg ccagcaggtg cgtcagaact aatcccagca gcgcgcccac
     9901 aattaacaac gcattaagca tcaggctggc agtcagcgca tcttccagac caatagtctg
     9961 ggcgatagtt ggcagccagg tataaatcac aaaccatggg attacgaggc agacaaagaa
    10021 gacgctgtta aacgccgtgc gccgccagta acgcgaagag aacaaggttt tgatgtgttt
    10081 atgggtcgcc gttaccactt catcgcccag taaaacatgg ggaccaaaat agcgatgcac
    10141 gatagcgtga gcttctgcaa aacgcccctg gcgcagtagc cagcgtggcg attctggcgt
    10201 tccccagcgt aataacgtaa tcaacaacgc gggcagagct gccgatgcca gcagccagcg
    10261 ccaggcctcc gggttttcgg aaataaagtg atgtccggca atacttgcca gcacatagcc
    10321 tacggtccac accacgctga atgcgcccag caaaacaccg cgatggcggc gcggggaaaa
    10381 ttcagccagc aaggtgtgac ctactgagta atcgcctccc agaccaatgc cgatcaaaat
    10441 gcgcagccca ataagatgct ctggcgtggt ggcaaaaaat tgcaggaacg aagcgagcgt
    10501 aatcagcaaa aagctgaagg tgaagatttt ttgccgacca atatggtcgg agatccaccc
    10561 aagaaccagg ctgccaagaa acaaaccaag gagcgccgag ccgccgatca ttcccgccat
    10621 aaacggcgtc agttgcatgg cgggcgtaag ttgaataatg gcgtaaccaa tgacgccgag
    10681 aacataaccg tcggtcaggt gtgcgccgaa agtgagcgca gcaattcgac agtgaaaacg
    10741 gttaagcggt aaatcatcca ttcgcaccgg tgaagtgttc atctttaccc cttcattgtc
    10801 cggtaataag aacggcttct cgcctgagaa gccgaaaagg caacgcccca tcatcgcgga
    10861 acgctgcctg acacattatt tttcaatcgg atagatagta cccgtattca taatgccgtt
    10921 aggatcgaat tgctttttca gcccttccag caacgcccac gcgctgccgt gttccagctt
    10981 gctccagtga acgcgatgtt taccaatgcc gtggtggtgc accatcgaac cgccgaggcg
    11041 aatggtttct tcgcagatga ttttgttgag cggattgtgg tacttgtcga tttcctcttc
    11101 cggcttacag ttgacaacgt tgtagtcgta gacgaagtac atgttggtgc cgttctgata
    11161 gctatgagag gagtgaccac ccagcatagt gatgtcgtcg gcgtgcggga actcggtacg
    11221 aatacggtta ataacgcttt cgtagatttc gtggatgcag ctccagcagc cggacacttc
    11281 agtggtaaag cccatgttgc cggttttgag gatttgcaca cgttcggcag ccactttatc
    11341 cggtccccag ttcaggttgt taaaccaggt ttcgatcagc ttgctgtcca cgcgttggca
    11401 ttgcgggtag cgggcaacga tttccgcaat cccttcgccc gtcgccttcg caatgcgagg
    11461 gttaccttca gccataaaga tcagcacgca ttttccgtcg gcaaaatggg tgaagtgttg
    11521 ggtgccatct tcagcgtcat acaaacgagc gatcgacgga cgataccctt ccaccatgat
    11581 ttcacgcagg atgttgaagc cggttttcat gtcttccagg atatagccgt agaagaggtt
    11641 gttttccggg gtgaatttaa agattttcac tgttacttca gtgatatagc acaatgcacc
    11701 ttcgttgccg atgatgatgt gacgaatgtc cgggccagcc gcgcggcgtg gcacgttttt
    11761 aatacgtgtg acggtgccat cagccaatac ggcttccaga ccaacgacca tatcttcgat
    11821 tgcgccgtag agtgtggaga actgcccgat actgcgggtt gctaccaggc cgcccatctg
    11881 cgccagcggc tttgactgcg gagaatgccc cgtggtgtaa cctttttcac gcaacgcgtt
    11941 ttccagcacc tccagcggaa cgccacattg cgccgtcgcc tgcatattct caatatcaat
    12001 attaatgatt tgattcatgg cggaaccgtc gagcaccacc gagttttcta caacagtttc
    12061 cagcccacct tcggtggcgg aagcaccggt acgcggcaca ccgttaattt tgtgcgcatt
    12121 cataaaattc agcacacggg atacttgctc tgtggaaccg agttttacga ccgctgccgg
    12181 aatcggcaaa gtataaatgc catgaatatc cggaaattta cgaaaacggt caatactgtt
    12241 cttctttaat actgtttcat cggtaattac gcgatctgca ccaacaattt cctttagctg
    12301 gtcgacaatc gctgcgcgag ataaagacat agtaattcct tcctgataat aaaaatggaa
    12361 taatcattaa taagagaaaa cagaaaccac cgttatgggt tagcgcacta aataaccgcc
    12421 atcgaccact aataaatgcc cgttgacata attcgatgcc tgacttgcga gaaacacggc
    12481 tgcgcccatt aaatcctgag tatcgcccca acggtttgcc ggaatatgat caagaacgcg
    12541 ctgattggtt tctggattac tgcgtgtcgc cagcgtaata tctgttgcat aatagccagg
    12601 ggcgatacca tttacctgaa tattatattg acctagttca tcacaataag ctttggtgaa
    12661 tccggcaaga gcatgtttag tagcagaata tgcaggtgac cattgtccac ctaagtaaga
    12721 gaacaatgaa cagatattaa tgattttacc gcttttctgc gggatcataa tttttgccgc
    12781 ttcgtagctt aactcgaatg cggcggtcag gttcacatca atcattggat cccagtcggc
    12841 acgaccgaag tccagcacct tattcagctt acaaataccg gcattgttaa ccagaatatc
    12901 aactgtaccg aaacgctcac agcaggcagc gataatcttc tgcggcgcgc cttctgcggt
    12961 gatatccacc tgcatgaagt ccacctcaac accctgattt tcaatcattt cctttgtttc
    13021 accgttatct ttgacaaaac ttggaataaa gacatttgcg cctgctttgg ccaacgccat
    13081 ggcaaatgcc tggcctaaac cgctattccc gccggtaaca attgcggttt tacctttcag
    13141 ggagaaaaaa tccattgaga acgcattgag agattcgatt gacatagtta gctccaattt
    13201 ccgtaaggca aaaaaaaaga aaggtaactt cccccgcgag agggaagtta cctttctcat
    13261 tatctctggt aactggagat aatttttagc ataaggacat aactgctaat gtgatcgtaa
    13321 tcacagtgtg atattcgcat tatcaaatag ttttaaaaag cagaagcgtg attactctca
    13381 cacaacacct ctgctttcca tgttatttct tattgcaatt actagagacc tgagaaaagt
    13441 aatccacctg aaagggcggc ttacttttct cttttttttg tctattacaa atgggaatac
    13501 gatatgcaac acaactcata tcgccgttgg ataaccctcg cgataattag ttttagcggc
    13561 ggcgttagtt ttgacctggc ttatttacgt tatatttatc aaattcccat ggcgaaattt
    13621 atgggattca gcaataccga gataggttta ataatgagta cctttggtat tgcagctatt
    13681 attctttatg cccccagcgg cgttattgcc gataaattct cacatcgcaa aatgattact
    13741 tccgcgatga tcattaccgg attactgggt ctgttaatgg caacgtatcc accgctgtgg
    13801 gtaatgctct gtattcaggt cgcctttgcg ataacgacga ttttaatgct gtggtcggtg
    13861 tcgattaaag ccgcctcatt gctgggcgat catagtgagc aagggaaaat tatgggctgg
    13921 atggaagggc tgcgcggcgt cggtgtaatg tcgctggcgg tgtttaccat gtgggtcttt
    13981 tctcgctttg caccggatga cagcgccagc ctgaaaacgg tcattatcat ctatagtgtg
    14041 gtttacatct tgttggggat tctgtgctgg ttttttgtta gcgataacaa caacctgcgc
    14101 agtgccaata acgaagaaaa acaatcattc cagcttagcg acatcctggc cgttttgcgc
    14161 atcagcacca cctggtattg cagcatggtg atttttggtg tcttcaccat ctacgccatt
    14221 ctgagttact ccaccaacta tctgaccgaa atgtatggca tgtcgctggt ggcggcgagc
    14281 tacatgggga ttgtgatcaa caaaatcttc cgcgcgctgt gcggcccact tggcggcatt
    14341 atcaccacct acagtaaagt gaaatcccct acccgcgtgc tccaaatcct ttccgtactc
    14401 ggcctgctgg cgttaactgc cctgctcgtc acgaactcta acccgcaatc ggtcgcgatg
    14461 gggattggcc tgattttact gctgggattc acctgttacg cctcacgcgg gctgtattgg
    14521 gcctgccctg gcgaagcaag aacaccgtct tacattatgg gcaccacggt aggtatttgt
    14581 tcggtgatag gattcctgcc ggatgtcttc gtttacccaa ttatcggcca ctggcaggac
    14641 accctgcccg cagcagaagc ctaccgcaat atgtggctga tgggcatggc ggcgctcggc
    14701 atggtgatcg tctttacctt tttgttgttc caaaaaattc gtactgctga tatcgccccc
    14761 acaacggcta acagcaagta agccagaaaa attacaggag aaaaatcatg tcgaagaaat
    14821 acatcatagg gattgatggc ggaagtcaga gcacaaaagt ggtgatgtac gatctggaag
    14881 gtaacgtggt ttgcgaaggc aaaggtttat tgcaaccgat gcacacgcca gatgccgata
    14941 ccgcagaaca tcctgacgac gatttatggg catcattatg ttttgccggt cacgatttga
    15001 tgagtcagtt tgccgggaat aaagaagata ttgtcggtat tggtctggga tccatccgtt
    15061 gctgccgtgc gttattgaaa gccgatggca cgccagctgc gccattgatt agctggcagg
    15121 atgcacgcgt aacgcgtccc tacgaacaca ctaatcctga cgtggcatat gtcacctctt
    15181 tttcgggtta tctgatgcat cgcttaaccg gcgagtttaa agacaatatc gccaactatt
    15241 ttggtcagtg gccggtggat tataagacct gggcatggag tgaagatgct gcggtaatgg
    15301 ataagtttaa tatcccccgt catatgctgt ttgatgtgca aatgcctggc accgtcctcg
    15361 gacatatcac accacaagcc gcactggcga cacatttccc ggcaggactg ccggttgttt
    15421 gtaccaccag tgataaaccg gtagaagctc tgggggccgg attactggat gatgaaactg
    15481 cggtaatttc tcttggcact tacatcgcat tgatgatgaa cggcaaagca ctgccgaaag
    15541 atccggtggc gtactggccg attatgtctt ctattccgca aacattgctg tatgaaggtt
    15601 acggtattcg caaaggcatg tggacggtga gctggctgcg cgacatgcta ggcgagtcgt
    15661 taattcagga tgccagggcg caggatcttt caccggaaga tttactcaac aaaaaagctt
    15721 cttgtgtgcc gccaggctgt aatggtctga tgacggtgct ggactggctg accaatccgt
    15781 gggaaccgta caaacgcggg attatgatcg gctttgattc cagcatggat tacgcatgga
    15841 tatatcgttc gatactggaa agcgtggcgc tgacgctgaa gaacaattac gacaatatgt
    15901 gtaatgaaat gaatcacttt gcgaagcatg taatcattac tggcggcggt tcgaacagcg
    15961 atctgtttat gcagattttt gccgacgtgt tcaaccttcc ggcacgacgt aacgccatta
    16021 acggttgtgc aagtctgggg gcagcgatta atacagcggt aggtctgggg ctatacccgg
    16081 attacgcaac ggctgtcgat aaaatggttc gcgtgaaaga tatctttatg cctgttgaga
    16141 gcaatgccaa acgctacaac gcgatgaata aaggcatttt caaagattta accaaacaca
    16201 ctgatgtgat tctgaaaaaa tcgtatgaag tgatgcatgg ggaattgggg aatgcggatt
    16261 cgatccagag ctggtcgaat gcgtaagtga ggaagaccgg gcgggaaact gcccggcctg
    16321 aacatacctg aatggttatc cccgctggcg cggggaacat aagttcgaca acttaaaaaa
    16381 tgataacaag tccggtttat ccccgctgat gcagggaaca taataaaaac tttaactagt
    16441 tcggtttatc cctgttttta caaggcatat taactacact cgatgtcttt agaataaaag
    16501 acaatacgaa tcgcacttta tcaattttca cttaaagtat gaactggata tctttattca
    16561 taagaaatat tcatcaacag cgtatagagg cggttatgta aaaccactca ttagcctcaa
    16621 aacaaagaca aatttccaga tttattactt aatcaatgaa taacaagctt
//
LOCUS       DAZFKF010000106        16481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFKF010000106 DAZFKF010000000
VERSION     DAZFKF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.106"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            125..748
                     /locus_tag="ACHKA1_004174"
     CDS             125..748
                     /locus_tag="ACHKA1_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5681746.1"
                     /translation="MRTRIDYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYYL"
     gene            837..1070
                     /locus_tag="ACHKA1_004175"
     CDS             837..1070
                     /locus_tag="ACHKA1_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681747.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(1090..1299)
                     /locus_tag="ACHKA1_004176"
     CDS             complement(1090..1299)
                     /locus_tag="ACHKA1_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5681748.1"
                     /translation="MSEDIPLPKINQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPRKEPPAGNHTA"
     gene            1798..2295
                     /gene="tssB"
                     /locus_tag="ACHKA1_004177"
     CDS             1798..2295
                     /gene="tssB"
                     /locus_tag="ACHKA1_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO5681749.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGGQKKVELPLKLLVAGDFSNG
                     QEERPLSERIKMDVNKNNFNAVLADISPSVRLNVENTLAGDGSEESISLTFRNMNDFT
                     PEQIARQIPRLKAMLAMRSLLRDLKANLLDNATFRKELEKILRDPELSQALRDELSAL
                     VPEQV"
     gene            2316..3860
                     /gene="tssC"
                     /locus_tag="ACHKA1_004178"
     CDS             2316..3860
                     /gene="tssC"
                     /locus_tag="ACHKA1_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO5681750.1"
                     /translation="MLMSVQKENNVAGNAVSETHSGDSVYASLFEKINLNPVSALSAL
                     DIWQDPQAMSEASADERLTAGMQVFMECLAKAGAPVEKLDKALIDHHIAELDYQISRQ
                     LDAVLHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDFEDAPEIIQS
                     GLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASAHMPFIGSVGP
                     AFFRKDTMEEVAAIKDIGNHFERAEYIKWNAFRETDDARYIGLVMPRVLGRLPYGPDT
                     VPVRSFNYVEEVKGPDHHKYLWTNASFAFAANMVRSFVTNGWCVQIRGPQAGGAVQDF
                     PIHLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPA
                     LYDTPDATANSRINARLPYIFLLSRIAHYLKMIQRENIGTTKDRRLLELELNNWIRGL
                     VTEMTDPGDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQ
                     MPKAKA"
     gene            3876..5213
                     /gene="tssK"
                     /locus_tag="ACHKA1_004179"
     CDS             3876..5213
                     /gene="tssK"
                     /locus_tag="ACHKA1_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO5681751.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSDRSLVDIVLA
                     LPLLNANGGNLDDGRESGRPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVRDAQGQWCRDPRFIPPLLTLSASPSLMTELAELLHHLQARRQRLMSM
                     RRENNARLADFAVADVSLFWLLNALNSAEPVLKELLDMPYRHPELLYRELARLAGSLL
                     TFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLEASLPSRVVFIELKQKGVMWEGAL
                     HDARLREGADFWLSVRSSMPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTH
                     VPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
     gene            5210..5863
                     /gene="tssL"
                     /locus_tag="ACHKA1_004180"
     CDS             5210..5863
                     /gene="tssL"
                     /locus_tag="ACHKA1_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO5681752.1"
                     /translation="MNKPVISRAEQIFYPGWLMVCQLRSGQPVEDGKALYRRACQLVK
                     QAREELAEAGFSQENSDTMLYAFCALLDESVLNREKTDDGWHTWQQDPLQAHFFGTLN
                     AGEELWERIREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVP
                     AFTFAQDAPVVVRAPGYRAGRTMYWLSWVAGIMTLAGLWCVLSSVLADQVARLTGQG"
     gene            5866..7566
                     /locus_tag="ACHKA1_004181"
     CDS             5866..7566
                     /locus_tag="ACHKA1_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO5681753.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAG
                     LVLWYQWRCLSSRRDIPENAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGW
                     YLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
                     CRQWLNTMPPVLVTLMVSGPSPQNTSLNKHSAHWFTLTPDLSGMHIRLTEAGAIPAEE
                     WLRECDAASRLRCVSELLWLNAMLSWYNRVTDALTDICRQEHFYFRPVAVGFCLTSVA
                     ARPGNLWQQQLTSLTVLPPPAGVSSQALPLPDILLSGIPRHRGLSHWHRLCLQTGAIV
                     GVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSESKKTALQRLQADSRLLNDWQ
                     QHGVPQRYGLGLYQGMWLIPPVERATGEWVAPLPPRPVIQKVTQTPKTVRLNSLALFD
                     AGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDPQRNQALSLKRAEAVRDW
                     MRDTGDIPQSCFAVQGYGESRPVAPNETAEGRARNRRVEISLVPQVDACRLPDITRAP
                     "
     gene            7601..8092
                     /gene="hcp"
                     /locus_tag="ACHKA1_004182"
     CDS             7601..8092
                     /gene="hcp"
                     /locus_tag="ACHKA1_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HFO5681754.1"
                     /translation="MAIPVYLWLQDDGGADIKGSVDVKDREGSIEVVAQEHCLYIPTD
                     NNTGKLTGTRIHTPFLFTKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYF
                     NTKLENVKVVKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSDAWNER
                     TTA"
     gene            8261..10948
                     /gene="tssH"
                     /locus_tag="ACHKA1_004183"
                     /gene_synonym="clpV"
     CDS             8261..10948
                     /gene="tssH"
                     /locus_tag="ACHKA1_004183"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO5681755.1"
                     /translation="MENPAALLRRLNHYCARALEGAASLCQTRAHAEITPEHWLLKLL
                     EQGEGDLTVLGRRYDWDMDAIWQSLLGWLDNQPRSVRSRPQLAPSLNALLKQAWMVAS
                     LQGEEHIRSVHLLGALTENPHLVRCDGLWPLLTLSHSQLQRLSPLLDAQSDECPERLQ
                     DAEPVLPQGDNVTFIGRPVGADTAGIPSGDLPPVLQSALDKFTRDITASAREGKIDPV
                     SGRDTEIRQMVDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRPVTLR
                     TLDLGLLQAGAGVKGEFEQRLKNVIDAVQQSPAPVLLFIDEAHTLIGAGNQAGGADAA
                     NLLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVRVDEPDDETACLMLRSLK
                     SRYAEHHNVHITDEAVRAAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTVPEQL
                     TRIRSQLASLGMEKQALLEDIAVGHQNHGERLSAIEQEENELMTARDDLEQQYARECE
                     LTGELLESRSDISRQSETHHLQQALHDIQQNQPLLSVDVDVRTVAGVVADRTGVPLSS
                     LMKDEQTELLHLEKDIGRRVVGQDVALESIAQRLRAAKTGLTSGNGPQGVFLLVGPSG
                     VGKTETALALADVMYGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAV
                     RKRPYSVVLLDEVEKAHRDVLNLFYQVFDRGFMRDGEGREIDFRNTVILMTSNLGSDL
                     LMQQLSEKPETTESELHELIRPLLRDHFQPALLARFRTVIYRPLTQSAMRTIVEMKLA
                     QVCERLHCHYGLSTSVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSYL
                     AARQHPTYLSLTWNDEDGIVIAFDGINSDYPQESIYGQC"
     gene            10935..11576
                     /locus_tag="ACHKA1_004184"
     CDS             10935..11576
                     /locus_tag="ACHKA1_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681756.1"
                     /translation="MDSAKEIARDMLEAVNINGDGFLGAVGKGLISLPVSLGYMGYDF
                     IDTGHRRENQNDKFRMARLVEKGIFSRDIIGKIISICLEDFVSRINMEKTGSIVKNVS
                     GDLAGRMAFAELTGVKLGEVLVSRGLAAFFAGSFAGLLLSVGAETSRAIYTSRFLKDR
                     NPVLHDKLKAQGDLDLLYFLVEDIVRPFERACIVADRNSAEFDRICEYFFGGL"
     gene            11573..11800
                     /locus_tag="ACHKA1_004185"
     CDS             11573..11800
                     /locus_tag="ACHKA1_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681757.1"
                     /translation="MKIKRTLLIMLSRFIRGAGMGLGASGIVFTVWFFFLSSAEFKYL
                     WGGLSVALFFVGYLMYRFAYAYIYDEWDDYQ"
     gene            11879..14398
                     /locus_tag="ACHKA1_004186"
     CDS             11879..14398
                     /locus_tag="ACHKA1_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO5681758.1"
                     /translation="MSLTDTLSKTISASLNRYHLIIPSCPSPLDVEHFSGREEMSQTY
                     YYIINFTSTDNDLDAGLLLRKSATFTMGTGGLKEQVSQKVVHGVITDFRRISGSADQA
                     KYQVILEPFIKLLDRQHRSHRFFVNKSVPEVVTEVLQEHGLKGWEFEFRLKKTYPKRE
                     QINQYQESDLQFIQRLLAEVGIFYFFTLQPDTQTEVVHFGDSQAALTFDKTLAINSPS
                     GMNDNRADSVWGLNVTHNVAEASVTTKAYNHREAQYLLQSAPADMTHGDGDGINYGEV
                     YHYHFRHLERGDKIDPVPETANFYARLGHERYLAEQVRITGNSTDATLAPAQVLTITD
                     SLPPTLPALLRNPVLLTCVGFSASRKDALQVVLKGVPYSEVICWRPPLLPRPKVTGTM
                     TARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFP
                     LIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKR
                     GEEHIKISTEFGGKTQLNLGHNVNASRKLRGEGAELRTDDWISIRGGKGIFISADMQP
                     QAQGKMLDMNEAIRQLELALSLARSMAKAATAANATQGDISCQQRLNSSLTDLTAPGM
                     LLHAPDGIGMVSARALRIASGSESVGIMSGDNTDISAGQSFTVVAEGAVSLLSRNQGM
                     QLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGN
                     IELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
                     AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE"
     gene            14395..16077
                     /gene="tle1"
                     /locus_tag="ACHKA1_004187"
     CDS             14395..16077
                     /gene="tle1"
                     /locus_tag="ACHKA1_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector phospholipase Tle1-EAEC"
                     /protein_id="HFO5681759.1"
                     /translation="MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFD
                     GTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLEEINKNSISNGSYRGYYSNI
                     HWLNSLYVADQTLLESQVEAQIQTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
                     TDEAMERITQALSEFMGFNLNPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIA
                     RAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSIA
                     KKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSC
                     PAFEIVSDDTRETDTRVYRNAEQARKMLMTLPALKHILPHGKLTTKIRSVGVNNSNQR
                     RAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFAPIDPKNPDMNLSPE
                     LIPFVDKAIAQGKAVRLGQEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGE
                     IFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV"
     gene            16097..>16481
                     /gene="tli1"
                     /locus_tag="ACHKA1_004188"
     CDS             16097..>16481
                     /gene="tli1"
                     /locus_tag="ACHKA1_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HFO5681760.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGYKKLPYDEW
                     KFLFIYPKALPALATQALMVDGEGYKTTYQYLDTTRPSQVSVGRWSDHLGATESYYNK
                     GKALPLMLRFCWDSIIDKKSYETLIF"
BASE COUNT         3905 a         3781 c         4694 g         4101 t
ORIGIN      
        1 ctgatgccac ggggtattgc aacaatcatc cggtgatgga cgggagaaat atcgactctt
       61 ccgttagttt ttacagactg ataaagtttt gccagctgaa tctttatacg gaattctttt
      121 cagtatgcgt acacgaattg actatcttgc ggataaatac agttttactg aactgaatga
      181 acttccacgc cttcaccagc agtggcagga tgttctggag gagtgccggc agacagaggc
      241 cgggccggaa gaacggctgc gtattgcact gctgaatgtg gattacgtca ccagttttga
      301 actgcctttt cgcttgttgc ttacccggac accacaactg attgccgcgc tgcgtgaaga
      361 atgggatatc agccagaaaa atgtggtgtt caacgataaa cgctttggct gcgtgtacag
      421 cctgaaggcc agcctttccg gtgtgccgga tacataccgg tatcatctgt ctcatcgtat
      481 tcgccgggtg attgggaatg aaaacacatc atcgccatac cagcagattg cccgggaagt
      541 gaaagcgccc cgtgaacggc tgaagcatgc gctggaggcg gggttgctgg tgactgcact
      601 ggacggcctg ttctggttcg gcagtcagcg tattgcggct gatgtcctga ggttgagaaa
      661 gagcggaatg ccggtggtga caacgaccgc ggaggtgcat gataatctga cgggaacaac
      721 ccgcaaagta ccggcctact atctctgatt cgaccatcag ggggaatatt gctctggcag
      781 cagcagtacg acattcttta tccccgagcc tgaaaacatg tgctaaggaa acgggaatgg
      841 caaggtccga ttacgatatt atcaatctgt ctccggaaca tgaactgaat gagtggctgg
      901 cagagagagg ttatgccgga caggcggata accgtaaccg ggtggcagaa attgttaccc
      961 gcaaattgca ggacagcttt tatatcaacg tttcctggga tgcgctgaac acggcataca
     1021 gtgaacaccc ggagtggttt tctgggcttg tctccgggga tgagaattaa taagtggatt
     1081 atgctgccat caggcagtgt gattgccggc agggggttct ttccggggtt cgggtttgaa
     1141 ttttcccagg aagttgtaca tcggccgcat gcagggatgc ggatagccgt ctcgcataaa
     1201 caccacccgg gtttcctcaa cactggtcac ggtgacaagc gcaccatgat catctctgta
     1261 tcgctgattg attttcggta atggaatatc ttctgacata gatttcagaa cggtaaccgt
     1321 tcactaaatt ttcatgtggt atcactgaat caggagacga cgtttcctga tgtgcgttgt
     1381 taattttctg tttttaaaaa atattttctc tctcttcttt attttccgtg tcttttattg
     1441 ctctcctctt tcatgtcctt ctctttttct gatatgccct taaatgctgc cgaatcatca
     1501 ctcctcgtga atgtcattgc tggtaatcat tcggtataac catctgtatc aaaagcacta
     1561 accggaataa ttttctgcat ttttgtatta ctcctggata taagttttga taaatgctga
     1621 ttttgcgata tgtatcagaa taaagatctg gttattggca ttaaaacgat cgatatgtct
     1681 gtcatattga gaatttgcag atcttcgtcc tataatgggt gaaattaaat cagtgcacaa
     1741 ggggaggcct tctgtggcga tgggctccct gggatgcagg tttcacagga gaacgtcatg
     1801 agcagttcgt ttcagaatga gatcccgaag gcaagggtga atctgaaatt agacctacat
     1861 acaggtggtg ggcagaagaa agtggagctt ccgctcaaac tgctggttgc cggtgatttc
     1921 agtaacggac aggaagagcg cccgctgtca gagcgcataa aaatggatgt taataaaaat
     1981 aactttaatg ctgttctggc tgatatctcg ccgtcagtac gcctgaatgt ggagaacacg
     2041 cttgccggtg atggctctga agaaagcatc agcctgactt tccgcaatat gaacgacttc
     2101 acgccagagc agatcgcccg acaaattccc cgtcttaaag ccatgctggc catgcgcagc
     2161 cttctgcggg atctgaaggc gaatctgctg gataacgcca ctttccgtaa agaactggaa
     2221 aaaattctgc gggatcccga actgagtcag gccttacgtg acgaactgag tgcactggtg
     2281 ccggaacagg tgtgattaac ggattaagag agagcatgct gatgtctgta caaaaagaaa
     2341 ataatgtggc gggaaatgcg gtttctgaaa cgcattccgg cgacagcgta tatgcttccc
     2401 tgtttgaaaa aattaatctg aatccggtat cagccctgag tgcgctggat atctggcagg
     2461 atccgcaggc catgtcagag gcatcagcag atgaacgtct gaccgcaggg atgcaggtgt
     2521 ttatggaatg cctggcgaaa gcgggagcac cggtggagaa actcgataag gcgctgattg
     2581 accatcatat tgcagagctt gattatcaga tcagccgtca gctggatgcc gtgcttcatc
     2641 atccggaatt tcagaaagtg gaatccctgt ggcggggcgt gaagtctctg gtggataaaa
     2701 cggacttccg ccgtaatgtg aaaattgagc tgctggatct gtccaaagac gatctgcgcc
     2761 aggattttga ggatgcgccg gaaattattc agagtggtct ttatctgcag acttatgtgg
     2821 cggagtacga tacgccgggt ggtgagccca ttgcagccct gatttctgcc tgggagtttg
     2881 atgcgtctgc gcaggatgtg gcgctgctga aaaatatttc ccgggtggct gcgtccgcgc
     2941 atatgccatt tattggttca gttggcccgg ctttcttccg gaaggatacg atggaagagg
     3001 tggccgccat taaggatatt ggcaatcact ttgagcgtgc cgagtacatc aagtggaacg
     3061 ctttccgtga aacggatgat gcccgctata ttggtctggt gatgccacgt gttctgggac
     3121 gcctgccgta tggtcctgat accgtacctg tccgcagctt taactatgtt gaagaggtga
     3181 aaggtccgga tcatcataag tatctctgga caaatgcgtc ttttgctttt gcggccaata
     3241 tggtgcgcag ttttgtcacc aacggctggt gtgttcagat ccgcggtcct caggccggtg
     3301 gtgcggtgca ggattttccg atccatctgt atgatttggg aaccggtaat caggtcaaaa
     3361 ttccgtcaga ggtgatgatt ccggaaactc gggagtttga attcgctaat ctgggcttta
     3421 ttccgctctc ttactacaag aaccgggatt atgcctgctt cttctccgct aactccaccc
     3481 agaaaccggc actgtacgat acgccggatg caacggcgaa cagccgtatt aatgcccgtc
     3541 ttccctatat cttcctgctg tcccgcattg cgcattacct gaagatgatt cagcgggaaa
     3601 atatcggtac caccaaagat cgccgtctgc ttgagctgga gctgaataac tggatccggg
     3661 gtctggtgac ggagatgacc gatccgggtg atgagttgca ggcctcgcat ccgctgcgtg
     3721 acggaaaagt ggtggtggaa gacatcgaag acaacccggg atttttccgc gtgaaactct
     3781 atgctgtacc acatttccag gtggaaggta tggacgtcag tctgtcactg gtttcgcaga
     3841 tgccaaaggc aaaagcgtaa ggcaggcgtc gggaaatgaa gatttatcgt ccactgtggg
     3901 aagacggggc ttttctgatg ccccagcagt ttcagcagca ggctgcctgg gatgttcatc
     3961 tggcagacag cgttgcccga atggggctgg cgcatccctg gggcgtggtg gctgcagagt
     4021 ttgatgactc tctgttaccg ctttcacggt tgaatgccac ccgtctgatt gttcgtttcc
     4081 cggacggaac gcttattgat acggaacgtg cggataatct gcctccggtc tgtgatttat
     4141 cgacggtttc tgatcgttcc ctggtggata ttgtgctggc gctaccgtta ctgaatgcta
     4201 acggtggcaa ccttgatgat ggccgtgaga gcgggcgtcc gcgtcgctgg aaatcagaaa
     4261 gggtgaatgt gcaggagctg gccgggcatg agcaaagcga agtggcggta ttgcggcata
     4321 acctgactct gcgcatggct catcaggaaa atgctgcctg gctgacctgt cccgttaccc
     4381 gtcttgttcg tgatgcacag ggacagtggt gcagggaccc gcgttttatt ccccctctgc
     4441 tgacgctgtc tgccagtcct tcgctgatga ctgagcttgc ggagttgctt catcacctgc
     4501 aggcgcgtcg ccagcgactg atgtccatgc gtcgggagaa caatgcccgt cttgcggatt
     4561 ttgcagtggc ggacgtgtcg ttgttctggt tgctgaatgc cctgaacagt gcggagcctg
     4621 ttctgaaaga gttgcttgac atgccttacc gccacccgga actgttgtac agggagcttg
     4681 cccggctggc aggctccctg ctcacattct cgcttgagca taatgtggat gcggttccgg
     4741 cataccgtca tgaaacacct gaaaatgttt tcccaccttt gctttctttg ctgaaccagc
     4801 tgctggaggc aagcctgcct tcccgtgtgg tcttcattga actgaaacaa aaaggtgtga
     4861 tgtgggaggg ggcattacac gatgcgcgcc tgcgcgaagg agcggatttc tggctttcag
     4921 tgcgctcttc catgcccggg catgaactgc agacgaagtt tccgcagcta tgcaaagccg
     4981 ggagccccga tgatgtgtct gaggtggtga atgtggcgct gagcggggtc attatccgtc
     5041 cggttacgca tgtaccggcg gccattccgc tccggctgga aaaccagtat ttcgcgctgg
     5101 atttaagtac ggatgcggcc cgggcgatgc tggacgcggg gcgttgtact ttctacaccc
     5161 cggcatcgct gggagatgtg aaactggaac tttttgcggt gctgcggaca tgaataaacc
     5221 agttatttcc cgggctgaac agatttttta tcccggctgg ctgatggtct gccagctgcg
     5281 cagcggacaa ccggttgagg atggtaaggc cctgtatcgc cgtgcctgtc agctggtgaa
     5341 acaggcgcgt gaagagctgg ccgaagcagg attcagtcag gaaaacagtg acaccatgtt
     5401 gtatgccttc tgtgccctgc tggacgagag tgtgctgaac cgcgaaaaaa cagacgatgg
     5461 ctggcacacc tggcagcagg acccgctgca ggctcatttt tttggtacgc tcaatgccgg
     5521 tgaagagctc tgggagcgta ttcgtgagca actgaaatta ccagcgccgg atgtggcggt
     5581 actgacatgc ctctgccgta cgcttcagct cggttttacc ggtcagtacc ggtcgcagga
     5641 tgatgagcgt cgcgaagatg taatacgcgc actgacagcg cgtgttcctg catttacttt
     5701 cgcacaggat gcgccggtgg tggtgcgtgc accggggtac cgtgctggca gaacgatgta
     5761 ctggttgtca tgggtggcgg gtattatgac gctggctggt ctctggtgtg tgctttcttc
     5821 tgtgctcgca gaccaggtgg cacggcttac cgggcaggga taaccatgca cgtacgttcc
     5881 cgtctctggc tgacaatact gagtctgttc ctgacactgt ggctgatttc gggattcctg
     5941 ccactgtctg cagtaaacca gataatactg accctggttt cagttgtggc ggcggggctt
     6001 gtgctgtggt atcagtggcg ttgtctgtct tcccgtcgtg atatcccgga gaatgccggc
     6061 gatgacggtc tgccgcctga gtattttcag gggcatgtgt tgcttgttgc cggaaactca
     6121 gatgcgtggt tcgaacccgg tcaggcatac cgggaaacag catcgggatg gtatcttcgt
     6181 gtggatacgc cggagcagat gagaatactg gcagagcggt tgtcgtacag ccgtccggcc
     6241 ctgtgtggcc aggtcgtggt catgctggga cttttaccgg agcagcatac ctcagatgaa
     6301 gctttcctgt cgtggttgcg gaactggcag cgtgcagtca tgcagtgccg gcagtggctg
     6361 aataccatgc cgccggtgct ggtgacattg atggtttcag ggccgtcacc acaaaacaca
     6421 tccttaaata agcactctgc acactggttt acactgacac cggacctgtc gggaatgcac
     6481 atacgcctga cggaagccgg agccattccg gcagaggaat ggctccggga gtgtgatgca
     6541 gcgtccaggc tccggtgcgt cagtgaactg ttatggctga atgccatgtt gtcctggtac
     6601 aaccgggtta ctgatgccct gacagatatt tgccggcagg agcactttta tttccggccg
     6661 gttgccgttg gattctgtct gacgtcggtt gcggcccggc ccggtaatct ctggcaacaa
     6721 caactgacgt cactcactgt actgccaccg cctgccggcg tgtcatcaca ggcactgccg
     6781 ttgccggata ttctgctgtc cggtattccc cgtcatcgtg gactcagtca ctggcacaga
     6841 ctttgtctcc agacgggggc gattgtcggc gtttttcttc tgctggctat gctggcgtct
     6901 tttatgaata accagcgact ggttcgcagc gtgggggaac acctgacggc ctggcaccgg
     6961 atgcgcggat attcatctga atcgaaaaaa actgcactgc aacgtcttca ggctgacagc
     7021 cggttactga acgactggca gcaacatggt gtgccgcagc gttacggact gggattgtat
     7081 cagggaatgt ggctgatacc gccggttgag cgagcgactg gtgagtgggt tgcaccactg
     7141 cctccccgtc cggtgataca gaaagttact cagacgccga aaacggttcg ccttaacagc
     7201 ctggcgctgt ttgatgccgg taaatggacg ctgaaaccgg gcgccacaaa atggctggtg
     7261 aatgccctgg tggatattaa ggcaaaagcg ggctggctga ttgtggtcag tggtcacacg
     7321 gacaatacgg gtgacccgca gcgcaaccag gccctgtcac tgaagcgcgc agaggccgtt
     7381 cgcgactgga tgcgggatac gggcgatatc cctcaaagct gttttgccgt acagggatat
     7441 ggtgagagtc gtcccgtggc tccgaatgag acggcggaag ggcgagcgcg taaccgccgt
     7501 gttgaaatca gtctggtacc gcaggtcgat gcctgtcgtc tgccggacat cacccgggcg
     7561 ccgtaacagg gcgcgtaaga caacgagagg gagtaacttt atggcaattc ctgtttatct
     7621 gtggctgcag gatgatggcg gagcggatat taaaggctcc gtggatgtga aggaccgtga
     7681 gggcagtatt gaagtggtgg ctcaggagca ctgcctttac attccgacgg ataacaatac
     7741 cggtaagctg accggtaccc gtattcacac gccgttcctg tttaccaagg aaattgattc
     7801 ttccagcccc tacctttaca aggcggtgac cacggggcag accctgaagt ctgctgagtt
     7861 taagtggtac agaatcaatg atgccggtca ggaagtggag tatttcaata cgaagcttga
     7921 gaacgtgaag gtggtgaagg ttaacccggt catgcacgac atcaagaatc ccgcctacga
     7981 gaagcataac caccttgagc aggtggagct gcgctacgag aaaatcacct ggacttataa
     8041 ggacggtaac atcattcatt ctgacgcgtg gaatgaacgt accactgcgt aattctcccg
     8101 gacgggcagg gtatcctgtc cgtcattttt cttttttctt cagtatctgc tggcgggtgc
     8161 tgaagaaaga attctcagtc ctgaacacag acctgatgcg cccgggttac ggcgaagggc
     8221 ggtagcgtgc ctgacggtct cctgacagtg agtgaataac atggaaaatc cggcagccct
     8281 gttacgtcgt cttaatcatt actgtgcccg tgcactggaa ggcgcagcct ccctttgcca
     8341 gacccgggcc catgcagaaa tcaccccgga gcactggtta ctgaaactgc tggagcaggg
     8401 ggaaggagac cttaccgtgc tgggccggcg ttatgactgg gatatggacg cgatatggca
     8461 gtcgctgctc ggctggctgg ataaccagcc ccgtagcgta cgcagccgcc cgcagcttgc
     8521 gccgtccctg aatgccctgc tgaaacaggc ctggatggtg gcctcattac agggagaaga
     8581 acatatccgc agcgtgcatc tgctgggtgc cctgacggaa aatccgcacc tggttcgctg
     8641 tgacgggctg tggccgctgc tgacactgag tcatagccag ttgcagcgtc tgtccccgct
     8701 gctggatgcg cagtctgatg agtgtccgga gagattacag gatgcagagc ctgtgctgcc
     8761 tcagggagac aatgtgacct ttatcgggcg tcccgtgggt gcggatacgg cgggtatacc
     8821 gtcaggtgac ctgccgcctg tgttacagag tgctctggat aaattcaccc gggacatcac
     8881 ggccagcgcg agagagggga aaattgaccc ggtatcggga cgcgatacgg aaatccgtca
     8941 gatggtggat attctctccc gccgccgcaa gaacaacccg attctggtgg gggaccctgg
     9001 tgtggggaaa acggctctgg tggaagggct cgccctgcgt attgcggaag gtaacgtacc
     9061 ggaatcactc agacccgtca ccctgcgcac ccttgacctc ggcctgctgc aggccggtgc
     9121 cggcgtgaag ggtgaatttg aacagcgcct gaaaaacgtg attgatgccg tgcagcagtc
     9181 accggctcct gtactgctgt ttatagatga agcccatacc cttatcgggg ccggtaatca
     9241 ggccggcggc gcggatgcgg ccaacctgct gaagcctgcg ctggcgcggg gtgaactgcg
     9301 taccattgcc gccaccacct ggtctgagta caaacaatac tttgaacgtg acgcggctct
     9361 ggagcggcgt tttcagatgg tcagagtgga cgagccggat gatgagacgg cctgtctgat
     9421 gctgcgctcc ctgaaatccc gttatgcgga acatcataac gtgcatatca ccgatgaggc
     9481 ggtacgtgct gccgtcacgc tgtcgcgccg ttatctgaca gaacgccagt taccggacaa
     9541 ggctgttgac ctgctcgata ctgctgctgc ccgtgtgcgt atgagcctcg acacggtgcc
     9601 ggaacagctg acccgtatcc gttcacagct cgcttccctc ggtatggaaa aacaggcact
     9661 gctggaagat attgccgttg gtcatcagaa tcacggcgaa cgtctgtctg ccattgagca
     9721 ggaagagaac gagctgatga cagcacgtga tgacctggag cagcagtacg cccgggagtg
     9781 cgaactcacc ggtgagttac ttgaaagccg cagcgatatt tcccgccaga gtgagacaca
     9841 tcacctgcag caggcactgc acgacattca gcagaatcag cccctgctca gtgtggatgt
     9901 tgacgtacgc accgttgccg gtgtggtcgc ggaccggacc ggggtgccgt tatcttccct
     9961 gatgaaggac gaacagacgg aactgctgca tctggaaaag gatatcggca gacgggtggt
    10021 cggacaggac gtggcactgg aatccattgc gcagcgtctg cgtgcggcga aaacgggtct
    10081 gacatccggt aacggccccc agggggtgtt cctgctggtc ggtcccagtg gtgtgggaaa
    10141 aaccgaaacg gcactggcgc tggcagacgt gatgtacggc ggcgaaaaat cactgattac
    10201 catcaatctg tcggaatacc aggagcccca tacggtttcc cagcttaagg ggtcaccgcc
    10261 cgggtacgtg ggatacggtc agggcggtat tctgacggag gctgtgcgta aacgccctta
    10321 cagcgtggtg ctgctggatg aagtggaaaa ggcccaccgg gatgtgctga atctgttcta
    10381 ccaggtgttt gaccggggct ttatgcgcga cggtgagggg cgtgaaattg acttccgtaa
    10441 tacggtcatt ctgatgacct ccaatctggg cagtgacctt ctgatgcagc aacttagcga
    10501 aaagccggag acaacggaat cggagctgca tgagcttatc cgtccactgc tgcgcgacca
    10561 cttccagcca gcactgcttg cccgtttccg gaccgtgatt taccgtccgc tgacgcagtc
    10621 tgccatgcgc accattgtgg agatgaagct tgcacaggtc tgcgaacgcc tgcactgcca
    10681 ttatgggctg agcacatcgg ttgatgaacg tgtgtacgat gccctgacat ccgcctgcct
    10741 gctgccggat acgggcgccc ggaatgtgga gagtctgctg aaccagcaac tgctgccggt
    10801 actgagccgg cagttgctca gctatcttgc tgccagacag catccgacgt acctgtctct
    10861 gacgtggaat gatgaggatg gtattgtgat agcgtttgat ggaatcaata gcgattatcc
    10921 gcaggaaagc atatatggac agtgctaaag aaattgcccg ggatatgctg gaggccgtaa
    10981 atattaatgg ggatgggttt ctgggggccg tcgggaaggg actcatatct ttgccagtca
    11041 gtctcggtta tatgggatat gattttatag ataccgggca taggcgggaa aaccagaatg
    11101 acaaattcag aatggccaga ctggtagaaa aaggcatttt cagccgtgat attattggga
    11161 aaattatcag tatttgtctt gaagactttg tatcccggat caatatggaa aaaacaggct
    11221 ccattgtgaa gaacgtttca ggagatctgg caggtagaat ggcatttgct gaattgaccg
    11281 gggttaaact gggagaggtt ctggtatcca gagggctggc tgcttttttt gcagggagtt
    11341 ttgccgggtt gttattatct gttggcgcag agacatcgcg tgcgatttac acatcccgtt
    11401 ttctgaaaga cagaaatccg gtgttgcatg ataagctgaa agctcagggg gatttggatc
    11461 ttttgtactt tcttgttgaa gatattgtgc gcccgtttga acgagcctgt attgtcgcag
    11521 accgaaattc ggctgagttt gaccggatat gcgaatattt tttcggtggt ttatgaagat
    11581 aaaaagaaca ttacttataa tgctttcccg atttatccgt ggcgcaggta tggggttagg
    11641 tgcgtcgggt attgtattta cagtctggtt cttttttctc tcatctgctg aatttaaata
    11701 tctgtgggga gggctctctg ttgcgttatt ttttgtcggt tatctgatgt atcgatttgc
    11761 atatgcatat atttacgatg aatgggatga ttatcagtaa ttctctctga aaataaagct
    11821 taacggtcat cattatataa ttgcagttca ataccggtac agacatggag ttaacaatat
    11881 gagtctgaca gatacacttt caaaaactat ctctgcttcc ctgaatcgtt accatttaat
    11941 tattccctcc tgcccgtcac cactggatgt tgagcatttc agcggcaggg aggagatgag
    12001 tcagacctat tattacatca tcaattttac cagtactgac aacgacctgg atgccgggct
    12061 gctgctgcgt aaatcagcca catttacaat gggtacggga gggcttaagg agcaggtgag
    12121 ccagaaagta gtacatggtg tcattacaga ttttcgtcgt atatcgggct ccgccgacca
    12181 ggcaaaatac caggtgatac tggaaccatt tattaaacta ctggacaggc agcatcgttc
    12241 tcaccgattt ttcgttaaca aatcggtgcc ggaggtggtg acagaggttc tccaggagca
    12301 cggcctgaaa ggctgggagt ttgagttccg tctcaaaaaa acgtatccga agcgtgaaca
    12361 aattaaccag tatcaggaaa gtgacctgca atttattcag cgtttgctgg ctgaagtggg
    12421 gattttttat tttttcaccc tgcaaccgga cacgcagacg gaagtagtgc attttggtga
    12481 cagccaggct gcgctgacgt ttgataagac gctggcgata aacagtccgt ccggaatgaa
    12541 tgataacaga gcggattcag tgtgggggct gaatgtgacg cacaatgtgg cagaggccag
    12601 tgtcaccaca aaggcttata accatcgtga agcacaatat ctgttgcagt ccgccccggc
    12661 agacatgacg catggtgatg gtgacggaat taactatggt gaggtgtacc actaccattt
    12721 ccgtcatctg gaacgggggg ataaaatcga cccggtgccg gagacggcca atttttatgc
    12781 ccgccttggt catgaacgtt atctggcgga gcaggtgcgg ataaccggca acagtacaga
    12841 cgccacgctg gcacctgctc aggttctgac tatcaccgac agtcttccgc cgacgttgcc
    12901 tgcgctgctg cgcaatccgg tgttgcttac ctgtgtcggt ttttctgcaa gtcgcaagga
    12961 tgccctgcag gttgtactga agggcgttcc ttacagtgaa gttatctgct ggcgtcctcc
    13021 tttactgccg cgtccgaaag ttaccggtac catgacggcc cgtgtgacca gtgcaaaaga
    13081 aggggatatc tacgcctggc aggatgcatc aggtatgtac cgggtgaaat ttgatgctga
    13141 ccgtgatgat aaaaatccgg gacaggaaag catgccggta cgtctggcca aaccctacag
    13201 tggtgatgct tatggttttc attttccgct gattcaggga acggaagtgg ccattgcctt
    13261 tgaggaaggt gatccggatc gcccctatat cgcgcatgcc ctgcatgatt cacgtcatgt
    13321 tgatcacgtt accgataaga acggtacgcg caatgttatc cgtacgccgg caaataacaa
    13381 gttgcggatg gaggacaaac gcggtgagga gcatatcaag atcagtaccg agtttggcgg
    13441 taagacacaa cttaatcttg gtcataacgt taatgcctcc cggaaactca ggggagaagg
    13501 cgctgagctg aggactgatg actggataag tatccgggga ggaaaaggaa tatttatcag
    13561 tgcagatatg cagcctcagg cacaaggaaa gatgctggat atgaatgaag ctatccgcca
    13621 actggagctg gcattatctc tggcccgaag tatggcgaag gccgcaactg ctgcaaatgc
    13681 tactcaggga gatataagtt gtcagcaacg gttgaattca tcgctaacag atctcactgc
    13741 gccgggaatg ctgttgcatg ctcctgatgg gattggcatg gttagtgcac gggcgttacg
    13801 aattgcttcg ggtagtgaaa gcgtgggcat aatgtcggga gataacactg atattagtgc
    13861 cgggcagtca tttacagttg tagcagaagg tgctgttagc ctgttgagca gaaaccaggg
    13921 gatgcaactg ttagcagcaa aagggcgggt aaatattcag gctcaaagtg acgatttgtc
    13981 aatgagttcc caacagaatc ttgatattca aagttctgag ggcaaagtga ctgtttctgc
    14041 taatcaggaa ctgatactgg catgtggtgg agcttatatc aaactaagcg gtggaaatat
    14101 tgagctggga tgcccgggac agatcctgtt aaaaagcacg aatatgcaga agatgggacc
    14161 aacaagtctt gatattgcgt ctgttgaaat gccgcgaggg tttggtggcg gttttattct
    14221 taccgatgaa gcgggagtgc cgcaaccttc gacgccttat cgattaacaa cggctgaagg
    14281 tgatattcta caggggatca ccgatgaaaa tggtaaaaca gcgccagtca acacctccat
    14341 tccgtcagta gtaaaagttg agtttgggaa ggtaaaaatt catggagaaa cagaatgaca
    14401 aaatatcagg gatatgatgt aacagatgcc acccataaaa ccagtattca taatgactgg
    14461 aaagttgttg ttgcaaagaa aaaaccggcg cggggtgtga ctttaaccat tggtatattt
    14521 ttcgatggta ccggaaacaa tcgggagaat acagcatctc gtctgatgaa atttaatgag
    14581 tgtagtgcag ccagacaggg ggtaaatcaa aaagatgcac aaagctgtga ggactttctt
    14641 gaagaaatta acaagaacag tatctcgaat ggcagttatc gtggatatta ttccaatatc
    14701 cactggctaa attcacttta cgtagcagat cagactttgt tggaaagtca ggttgaagca
    14761 caaattcaga cttacatttc cgggataggg actgcggctg gtgaagcaga ttcagtcatt
    14821 ggcatgggat taggcaccag tattctggat atatttgaag gtgtggtgac gaaaaccgac
    14881 gaggcaatgg aacgaatcac ccaagcgtta tcagaattta tggggttcaa tttaaatcct
    14941 gatttttgta tcgcaaaaat ccagtttgat gtttttggtt ttagccgggg agcagcggcg
    15001 gcaaggcact ttgctaatcg ggttatggaa caggatcctg ctatagccag agctattgct
    15061 aaagggctca gaggcgattt ttatgatggt aaaccttcgg gtgaagtccg ttttctgggg
    15121 ctttttgata ctgtcgcagc tattggtgga atcagtaatt tcttcgatat taatggacgt
    15181 agcaacccgg gagtaaagct ggaattgcgt ccttccatcg cgaagaaagt tttccagatt
    15241 actgcaatga atgaataccg atataatttc agcctgaaca gtattaaagg tatgtggccc
    15301 gaactggctt taccaggggc gcattcagat attggaggtg gttacaatcc ggtaggttca
    15361 cctctgcagg aaaatgaaag tctgtttctg agttgcccgg catttgaaat tgtcagtgac
    15421 gataccagag aaacagatac gcgagtatac cgtaatgcag agcaagccag aaaaatgttg
    15481 atgactctgc cagccttaaa acatattttg cctcatggaa aactaaccac caaaattcgt
    15541 tccgtaggtg tgaacaacag caatcaacgt cgggctggcg tgatacaaaa gcaggtggga
    15601 gccgcagtat tttttgagcg tatggcagta cctaatgact gggctaatgt ttgcctgcgt
    15661 gtgatgctgg atgctgctca ggaagcaggg gtattgtttg ctccaattga ccccaaaaat
    15721 ccagatatga atttatctcc tgaattaatt ccttttgttg ataaagccat tgctcaggga
    15781 aaagcggtac gtttggggca agagcctcag gcttttactg aggaagagtt gtatattatc
    15841 ggaaaataca cccattgttc tgccaactgg aatattgagt ctgatggtaa tttatgggtg
    15901 gatcctacaa cgggagagat ttttatccat cgctttggtc ctaaaggtaa taaggcgttc
    15961 gtttttccga ataaaccaaa tgatcgctgg attcgttctg tatggtacat ggatgatcag
    16021 cagcgattga atgataacgc agtaaaaaat actaaagtta tgatgtctgg tgtttaacgc
    16081 tacaggatga caatacatga ccagagataa aatcattttt atcgcaccat tattggcatt
    16141 gttaactggg tgtaattctg ttagctcaca gtatgctgat tcggataaga ctgaatgggg
    16201 ttataaaaaa ttaccctatg atgaatggaa atttctcttt atttatccaa aggctctgcc
    16261 tgcgctggcc actcaggcat tgatggtgga cggggaaggt tataagacga cttatcagta
    16321 tcttgatacc acaagaccga gtcaggtcag tgtagggcgc tggagtgacc atctgggcgc
    16381 gactgagtcg tattataaca aggggaaagc attacctctg atgttgcgtt tttgctggga
    16441 ttcaataatt gataaaaaga gttatgaaac tctgattttt t
//
LOCUS       DAZFKF010000107        15714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFKF010000107 DAZFKF010000000
VERSION     DAZFKF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.107"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(70..1186)
                     /locus_tag="ACHKA1_004189"
                     /pseudo
     CDS             complement(70..1186)
                     /locus_tag="ACHKA1_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1811..2236)
                     /gene="arsC"
                     /locus_tag="ACHKA1_004190"
     CDS             complement(1811..2236)
                     /gene="arsC"
                     /locus_tag="ACHKA1_004190"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO5681761.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDQL
                     VKLIADMGLTVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2569..2742)
                     /locus_tag="ACHKA1_004191"
     CDS             complement(2569..2742)
                     /locus_tag="ACHKA1_004191"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681762.1"
                     /translation="MHPLTHPLPVTVHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHCQSENASELTG"
     gene            2770..2853
                     /gene="dinQ"
                     /locus_tag="ACHKA1_004192"
     CDS             2770..2853
                     /gene="dinQ"
                     /locus_tag="ACHKA1_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO5681763.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(2907..4259)
                     /gene="gorA"
                     /locus_tag="ACHKA1_004193"
     CDS             complement(2907..4259)
                     /gene="gorA"
                     /locus_tag="ACHKA1_004193"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO5681764.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4331..5173)
                     /gene="rlmJ"
                     /locus_tag="ACHKA1_004194"
     CDS             complement(4331..5173)
                     /gene="rlmJ"
                     /locus_tag="ACHKA1_004194"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO5681765.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKSDGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5376..7418
                     /gene="prlC"
                     /locus_tag="ACHKA1_004195"
     CDS             5376..7418
                     /gene="prlC"
                     /locus_tag="ACHKA1_004195"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO5681766.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7426..8178
                     /gene="rsmJ"
                     /locus_tag="ACHKA1_004196"
     CDS             7426..8178
                     /gene="rsmJ"
                     /locus_tag="ACHKA1_004196"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO5681767.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8227..9696)
                     /gene="dtpB"
                     /locus_tag="ACHKA1_004197"
     CDS             complement(8227..9696)
                     /gene="dtpB"
                     /locus_tag="ACHKA1_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO5681768.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGSKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMVAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            9862..9915
                     /locus_tag="ACHKA1_004198"
     CDS             9862..9915
                     /locus_tag="ACHKA1_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681769.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            9893..9946
                     /locus_tag="ACHKA1_004199"
     CDS             9893..9946
                     /locus_tag="ACHKA1_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681770.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(10013..10447)
                     /gene="uspA"
                     /locus_tag="ACHKA1_004200"
     CDS             complement(10013..10447)
                     /gene="uspA"
                     /locus_tag="ACHKA1_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO5681771.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            10838..11173
                     /gene="uspB"
                     /locus_tag="ACHKA1_004201"
     CDS             10838..11173
                     /gene="uspB"
                     /locus_tag="ACHKA1_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO5681772.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11244..12743)
                     /gene="pitA"
                     /locus_tag="ACHKA1_004202"
     CDS             complement(11244..12743)
                     /gene="pitA"
                     /locus_tag="ACHKA1_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO5681773.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            12975..14177
                     /gene="yhiN"
                     /locus_tag="ACHKA1_004203"
     CDS             12975..14177
                     /gene="yhiN"
                     /locus_tag="ACHKA1_004203"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO5681774.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVEKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(14492..15544)
                     /gene="yhiM"
                     /locus_tag="ACHKA1_004204"
     CDS             complement(14492..15544)
                     /gene="yhiM"
                     /locus_tag="ACHKA1_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO5681775.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4034 a         3822 c         4012 g         3846 t
ORIGIN      
        1 ttcttttacc agacggtatt tctatttcca ccccataatt tatatctttt tcatcaggaa
       61 gtggcctctt taaataaggc gtgcgtgaca ttttttttgg gttaagcgtt tcgtattgaa
      121 atacttcgaa ttgatattca gacatttctg cccatgtttg aattttattg tatcgggaga
      181 gtgtcgtaaa ggctttaact tgcttataac attgtttttg gaattcttgc tgatatttta
      241 atatgtcgtt gtatgaaaga ttggttatcc tggcctctaa aaatttaatt attgtcgaag
      301 caatttccgc ttttgctcgt aaaaagttgt cattaatggt taataatgtt gaaaataaac
      361 cttcgatttg tttgtcaaga tattgttcgt ctttatataa ttttggggta gggtgttctt
      421 tatgtgaaaa aatagtgtca cctaaatttg cgtcagtgaa atttatatta agtagatctt
      481 cgaaataaaa ccttgacaag gttagaacga acccggacag gttctgtcct gtcatattga
      541 aatcaataaa gttggttccg gcaaagactt tcatcctgtt tttattttca ctgtgctcaa
      601 tatataactt cgtcattttt tgatacaggc tttgaatatc ttttatatta gtcggtaagt
      661 cagctcgttg ttcagcaata aaattagtat ctgaaaacat ggtttggaca ctgaatctta
      721 ctggtattcc ctcttctacg ctaattttag ttatgattgc cttgtcgggt ttaggattag
      781 cgacaacaat gattcgacat tcagcacttt tgttcttttc gtatctaatg ttattaattt
      841 gattaagtat ttcctgtgcc tgccgcgtaa gtggtgtcga acttatcgat tcaatgttag
      901 taataagact tacaagttga gccacaactt ttgagtagtc tgcatctgta tatttggtgt
      961 tcgtgcggca cgcataaatt tgtgggaggt gttgccgcgc taaatcatat tgttcagggt
     1021 tgtatccttc tccagagaat gtggaattaa tgggttgttg ttcttcccga ttatcacaag
     1081 gccacatcgg tgtgtctggt gttttaacat tgtcggtatc tgttgtgcgg ttataaatat
     1141 cgccgtgata tgtatttatc atacctgggg taaagtcaat tgacataaca ttccttattc
     1201 attatctgtt ttatgctaag atttttaaat tggataatta aagcaggaat ggattttctt
     1261 ctacgcacaa taaatatcat gcgtcatatc ttcctgatat aattaccttt gacttccctt
     1321 ttatctccac ccggtaatat ttgtgtataa aaaagggcat tgatagtatc gtaaaggaaa
     1381 acgttaacaa ctatcaaaaa gcgctcattt taaaaagtta tgatgttctc cgatagtgtg
     1441 tgaaatgata tttttgtgat gttttagatg tgtaataata aaatgggtta aggtatctta
     1501 taattattga ataatttgtt gtgtaaagca ctgataaatc ataatccagt acgtctttaa
     1561 actgctgcac tgtatcgcgg ataactaagc agctcactga tacaccatgc tggacgcgat
     1621 aattggaatc tcacgcaggg agtaatgtgt aaatagcaaa atcgcctgat gcgctacgcc
     1681 tgtcaggcct acaaataaca tactattcat cgaattttca taactttgta ggtcggataa
     1741 gacgcgccag cgtcgcatcc gacataaata aagcgcactt taccaactat cggcagtgga
     1801 tatcgccgct ttatttcaac cgcttacccg cttcatcaac cactttctcg ccatcttcct
     1861 tggagaacgc gcctttttgc gcatctggca gaatttccag caccacttcc gaggggcggc
     1921 ataggcgagt tcccagcggc gtcaccacaa tcggacggtt aatcaggata ggatgttgca
     1981 gcataaaatc aattaaccga tcgtcagtaa atttatcttc tgcaaggccc agttcctcat
     2041 acggctcgac gtttttacgc agcagcgcgc gtacggtaag ccccatatcg gcaatgagtt
     2101 tgaccagttg atcgcgcgtt ggcggtgttt ccagataata gataatggtc ggttctgtac
     2161 cgctgttgcg gatcatctcc agcgtattac gcgacgtgcc gcaggccggg ttgtgataaa
     2221 tggtaatgtt gctcatatca gtatctcatt acaaagtgaa agagagacgt agcgccagcg
     2281 cagccagcgt tacaaaccaa agcggataag tcaaaaacat atatggttta acgaatgcgt
     2341 atgtgcagag gaaggtaata ggtgtgaatt ttgagttggc tattcatttg aaaggaggtc
     2401 cgaatcagga cgtaaaaaaa ccgactttgc gtcggttttt tactttccag ccctgagttg
     2461 gtggctctgg ctggagtgag atagccatca tctaacgcat caatgctaat accatactga
     2521 aactattgca aggacgtgct ggttttataa cctgcatgta ctgtatgatt atccagttag
     2581 ctctgaggca ttttcactct ggcaatgcgc ataaacgctt tcaaagtcct ggtcagaagt
     2641 acgggtggtg ccgttaactg atgctctggc cggagtgaga tagtcgtcat ctaacaatga
     2701 gacatgcacc gtgacaggca gtggatgagt aagcggatgc attctcactc catcgcatgg
     2761 agaaaacggg tgattgataa agcaatcatc gttcttgggg cgttaattgc gctgctggaa
     2821 ctgatccgct ttctgcttca gcttctgaac tgatagcgga aacgtaatta agggctaaga
     2881 gcacactact cttagccctt taacatttaa cgcatggtca cgaactcttc tgccgccgtt
     2941 gggtgaatgg cgacggtatt gtcgaagtct tttttggttg cccccatctt cagcgccacc
     3001 gcgaagccct gcaacatttc gtccatacca aaaccaatgc cgtgaatacc gacaatcttc
     3061 tcttctggcc caacgcacac cagcttcatg cggcacggct ggcggtgagt ggtgacggcg
     3121 gtatacatcg cggtgaaaga ggatttatac actttcacct gatcgtcgcc gtactgctcg
     3181 cgcgcctgcg gttccgttaa accaacagta ccaatcggcg gatggctgaa gaccacggtc
     3241 ggaatgttgc tgtaatccag atgctcatcc ggcttgttat taaacaggcg ttcagagagg
     3301 cggcgacccg ctgcaactgc caccggtgtc agttctactg cacccgtgtt atcgcccacc
     3361 gcgtaaatac cttcaatgtt ggtgttttga tatttatcga cgacgatata gcctttttcg
     3421 ttggttttaa cgccagcggc ttcgaggttg atgttgtcat tggcaggctc gcgaccaatc
     3481 gcccaaatca ggcaatccac cgtttcactg cgaccatctt ccagctccag cgtcaggcta
     3541 ccatcggcat ttttcactac cgctttcggg atggcgttgg tgtgcaactg cgggccttcg
     3601 gcgttcatca cttcaaccag cgtttcagag atcatcgggt cgaagctgcg cagcggcgca
     3661 tgtttacgca caaacagatg cgttttcgcg cccaggccgt taatcacgcc cgccagctca
     3721 acggcgatgt aacccgcgcc aacaaccgcc acgcgctctg gcaaagcagg aagggcgaag
     3781 aagccatcag aatcaatacc gtattccacg cccggaatat ccgggtggct cggacgaccg
     3841 ccggtggcga tcaggatatg atcggccgtg atggtttcgc cgtttacttc cagcgttttg
     3901 gcatcaacga agcgggcaaa gcctttgatt acatcaacgt tatttttacc gagcacgttt
     3961 tcataggaag tatgaatacg gtcgatatag gcggtacggc tggcgatcaa cgtttcccag
     4021 ttgaatttat tgatagtggt atcaaaacca taatccgggc cgtacatatg gatcgcttca
     4081 cggatttgcg ccgcgtgcca catcactttt ttcggcacgc agccaacatt cacgcaggtg
     4141 ccgcccagct ctttggcttc aatcagcgcg catttctggc cgtacatagc cgcgcggttg
     4201 atggaggcga taccgccgct gccgccgccg atggcgatgt aatcatagtg tttagtcatg
     4261 acagagtgtc cttatcgttg attaccgcga ttgtagcgcg agacgtaata ggtgccagca
     4321 atggctgcaa ttactccggc acgatccagc ttacggtggc gtgcccggtg ccagccggaa
     4381 ccagtttgct gtgcagccac ggcagcacgt tattcatctg ctgttccagc ttccacggcg
     4441 ggttaatcac aatcatgccg gaagcggtca tgccacggcg atcgctgtct ggcagtaccg
     4501 ccagttcaat ttgcagaatt ttgcgaatac cggtcgcttc cagatcgtgg atcatgcgct
     4561 taatttgctg acgcagcacc accggatacc acagtgcgta agtaccagtg gcgaaacgtt
     4621 tgtaaccttc tgctatcccg ctgaccaccg cttgatagtc ggttttcatt tcatacggcg
     4681 ggtcgataag gattaaacca cggcgggaaa ccggcggcag tttggcctta agctgctgga
     4741 aaccgtcgga tttttcgacg cgcgcacggc tgtctttctg aaattcagaa cgcagcaacg
     4801 ggtaatcgct cgggtgcagt tcggtcagtt gcaggctgtc ctgttcacgc agtaactgac
     4861 gggcaatcaa cggtgaaccg gggtagtaac gcaactgacc gctacggttg aagtgtttta
     4921 ccacattgat gtacgcctcc agctccgcag gcaaatcgtc ctgctgccag atacgggcga
     4981 taccttcgag atattcgccg gtacgctcgg catgttcgct acctaactga taacgcccgg
     5041 cgcctgcgtg ggtgtcgaga tagagaaacg gtttatcttt ctctttcagc gactcgatga
     5101 tcaggctctg aacggtatgt ttaagtacgt cggcgtggtt gccagcgtga aagctgtggc
     5161 gataactgag catgggtaaa ggtgttccgg taagtaaaaa tcggcccgta ttcggggcgc
     5221 acaaaagcct atcaggacag tataccgaaa agaggccgcc gccgcgaaag cgtaacgttt
     5281 ctcattgaaa ttcactacac ttaaccccat gctacacaca ttatgtaaag cgcctgttga
     5341 gcgcttcctt aacctcttta accaggactg cgctaatgac gaatccgtta ctgactccct
     5401 ttgaattgcc tccgttttct aaaattctcc cggaacatgt cgttccagcc gtgactaagg
     5461 cattgaatga ctgccgcgaa aatgtggagc gcgtagtagc gcaaggagca ccgtacacct
     5521 gggaaaatct ctgccagccg ttggcggaag tggacgatgt gttggggcgt attttctccc
     5581 cggtcagcca cctgaactcg gtgaaaaata gcccggaatt gcgcgaagcg tacgaacaaa
     5641 ctctgccgct gctgtcggaa tacagcacct gggtagggca acatgaaggg ctgtataaag
     5701 cgtaccgcga cctgcgcgat ggcgatcatt acgccacgct gaacacggct cagaaaaaag
     5761 cggttgataa cgcactgcgc gattttgaac tctcaggcat cggtctgccg aaagagaaac
     5821 aacagcgtta cggcgaaatt gccactcgtc tttctgaact gggcaaccag tacagcaaca
     5881 acgtccttga tgcgacgatg ggctggacca aactcgttac cgacgaagcg gaactggcgg
     5941 ggatgccaga aagcgcgctg gctgcggcaa aagcccaggc cgaagcgaaa gagctggaag
     6001 gctacctgtt gacgctggat atcccaagct acttgccggt aatgacctac tgcgacaacc
     6061 aggccttgcg tgaagagatg tatcgtgctt acagcacccg cgcttcagat caaggtccaa
     6121 acgccggtaa atgggataac agcaaggtga tggaagagat cctcgctctg cgtcacgaac
     6181 tggcgcaact gctgggtttt gaaaactacg cctttaaatc ccttgccact aaaatggcag
     6241 aaaatccgca gcaggtgctt gatttcttaa ccgatctggc aaaacgcgcg cgtccgcaag
     6301 gtgaaaaaga gctggcgcaa ctgcgtgcct tcgctaaagc cgaatttggc gtggatgagt
     6361 tgcagccgtg ggatatcgcg tactacagcg aaaaacaaaa acagcacctc tacagcatca
     6421 gcgacgagca gctgcgtccg tacttcccgg aaaacaaagc ggttaacggc ctgtttgaag
     6481 tggtgaaacg tatttacggc atcaccgcta aagagcgtaa agatgttgat gtctggcatc
     6541 cggatgtacg tttcttcgaa ctgtatgacg agaacaacga actgcgcggc agcttctacc
     6601 tcgacctgta tgcccgtgaa aacaagcgcg gcggggcgtg gatggatgac tgcgtaggcc
     6661 agatgcgtaa agccgacggt tcgctgcaaa aaccggtcgc gtatctgacc tgtaacttca
     6721 accgcccggt gaatggtaaa ccggcgctgt ttacccatga cgaagtgatc accctgttcc
     6781 acgagttcgg tcatggtctg caccatatgc tgacccgcat cgaaaccgcc ggtgtatccg
     6841 ggatcagtgg ggtgccgtgg gatgcggtcg aactgccgag tcagtttatg gaaaactggt
     6901 gctgggagcc ggaggcgctg gcgtttatct ccggtcacta tgaaaccggc gaaccgttgc
     6961 cgaaggagtt gctggataaa atgctggcgg cgaagaacta ccaggcggcg ctgtttattc
     7021 tgcgccagct ggagttcggc ctgttcgatt tccgcctcca tgccgagttc cgcccggatc
     7081 agggagcgaa aatcctcgaa actctggcag aaatcaagaa actggttgcc gtagtaccgt
     7141 ctccatcctg gggccgtttc ccgcacgctt tcagccatat tttcgccggt ggttatgccg
     7201 caggttatta cagttacctg tgggccgacg tgctggcggc agatgccttc tcgcggtttg
     7261 aggaagaggg cattttcaac cgtgaaaccg ggcagtcgtt cctcgacaac attctgagcc
     7321 gtggcggttc agaagagccg atggatctgt tcaaacgctt ccgtggtcgt gaaccgcaac
     7381 tggatgcgat gctggagcat tacggcatta agggctgatc attcagtgaa aatctgctta
     7441 attgatgaaa caggcaccgg agacggtgcc ttatctgttc tggcggcccg ctgggggctg
     7501 gagcacgatg aagacaacct gatggcgctg gtgttaacgc cggaacatct ggaattgcgc
     7561 aagcgtgatg agccaaaact tggcggcatc tttgttgatt ttgttggcgg agcgatggcg
     7621 caccgacgca aattcggcgg tggtcgcggt gaggcggtgg cgaaagcggt gggcattaaa
     7681 ggcgattatt tgccggatgt ggtggacgct accgcagggc tggggcgcga tgcctttgtg
     7741 ctggcttcgg tcggctgccg cgtgcggatg ctggagcgta atccagtcgt tgccgcgcta
     7801 ctcgacgacg gcctggcgcg tggatatgcg gatgcggaaa tcggcggctg gttgcaggag
     7861 cgtttgcagt taattcacgc ctccagcctg acggcgctga ccgatattac tccgcgcccg
     7921 caggtggtct atctcgaccc gatgttcccg cataagcaga aaagcgcgct ggtgaaaaaa
     7981 gagatgcgtg tctttcagtc gctggtgggg ccggatctcg acgcggatgg gttgctggag
     8041 cctgcgcggc tgctggcgac taaacgcgtg gtggtaaagc gtccggacta cgcgccaccg
     8101 ctggcgaatg tcgccacgcc aaacgcggta gtcactaaag ggcatcgctt tgatatttat
     8161 gccggaacgc cggtgtaaaa gtaaagcagg gtagcgataa cgctaccctg ctttctgcaa
     8221 gaataattaa tggctttccg gcgtcgcaat catgcgtttc agccacggca ccatcaacag
     8281 catcactact gcaacgccca gcgtgaccag accaatctta ccaaacacgt tggtatagac
     8341 gggcaacgtc tcaagcggat cggtaatgtt gtccggcacc gcggtaaatg ttgccacata
     8401 gccgcccagc aagaacgcgg cagcctgcgt caggaaccac atcccgagaa taaagcccat
     8461 caaatgctgc ggcaccagag cggcaaccat cgccagaccc agggcgctaa taaacaattc
     8521 acccaggctc tggaacaagt aaaccagcac gataaaccac ggcgatgtca gtccttgtgc
     8581 atcagcaaac cacattcccg cagcggcggc ggtaagaaag cccagcgagc acataaacat
     8641 gccgagagta aatttcatcg gcatagagag atctttgcct ttgctgccca gatgcgtgta
     8701 aatgcctgcc agtattgggc tggcgagtac cacccagaac gggttaagcg cctggaagct
     8761 gaccgggttg atggaaaagc cgagaatttc atgatgcacg ttgttgatgg caaagaagtt
     8821 cagcgacgtt ggcatctggg cgtagagaat gtaaaacacc accgcttcga gcatcaggac
     8881 aaaggcgaca aacattttat tgcgcccggt tttatccagc ttgaatgcct gacgaaagaa
     8941 gatgatggtg acgacgatgg agagaacaat cagcaccaga ttggcgactt ctacgttgtg
     9001 catcagccat gcacagacga agatcatcac cacgctgcca agtaacacgt acaacagttt
     9061 gctgaagctc atcggccgga agtcgggttc agaaccaatg tctttcacca ttccacgaca
     9121 ggcgatgtaa accagtaatg cgataattaa ccccgccccg cacaggttgt aggtgacaga
     9181 ataaccgaat ctatcagcga tcacaggggc cagcgataac gctatcaacg agccgatgtt
     9241 gatcgacata tagaacaggg tgaatgcgcc atcaaggcgc ggatctttcg gcggatagca
     9301 cttcgaaagc aagctggctg ggttagcttt aaacaggccg ttaccgacag cgatagtccc
     9361 cagggcgatg aaaatcaggt caggcttaag tagcgacagg ccggtcatga agtagccaat
     9421 cgccagcaca agtgctccga gaacaatggt gcgtttggtc cccagcaggt ggtcgccgac
     9481 atagccgcca atggaaatga ggccatagac cagcgcagca aaagcaccaa aagtgacaaa
     9541 agcctgctct tgcgagaatc caagctgttt aacgaagaaa accgccagta cgccctgcac
     9601 gccgtagtag ccgaatcgct cccataactc gacaaaaaag atcatgaaaa atgggcgagg
     9661 ttgctgcagc atccccatgg gtgttgttgt attcatattt attaaccttc caataccatc
     9721 ctgaaaagta tgaacgcgta agggataacg cgcgaaaaag tttacatctg tcagagattc
     9781 ttaatattga tgcggtgaat agattaatac actattacaa cagaaaataa ccagctgatc
     9841 acactaatga tggaaaatat gatggtgtta aatattgtat tggctgggga ttttgattgc
     9901 gaacgcagac cgtaggccag ataaggcgtt tacgctgatc aggtaacaag gcgcgccaaa
     9961 aagcaaaacg cccgcatatg cgggcgttca ggaacacgtt gtggagggaa gattattctt
    10021 cttcgtcgcg cagcggaaca atcagcatat caacgtgaac ggtgttgatc agctgacgtg
    10081 cggaagacat cagtttgctc cagaagtcct ggtggtgacc acaaaccacc agatccatat
    10141 cgtatttctt gattgcatcg accagaacct ggcccaggtc gccgctgccg ctcagggttt
    10201 cagtgattgg gtatcctgca ttagtggaaa gctcggtcag cgcatgatgt gtctcttcag
    10261 agatgcgttt ctgcatatca cccagattca catcaataag cccggtgtat aggtcagagt
    10321 agtttacatc tacgtggatc agagaaactt tcgcattgta tgggcgagcc atagagactg
    10381 ctttctctac cagaactttg ctttccgggg agagatcgac cgcgatgaga atgtgtttat
    10441 aagccatagt gttactcctt ccataaagtt gtcgatgact ggccagctag cgtttcttgt
    10501 gccgttacgt cacccgcgtc ctgcgaacta cgtgcgcggg gctaacccag ccgtaaagat
    10561 tcagtgttaa gaccatcctt accttatagc gacccggatg atccgtcaat ccgccttgct
    10621 tacccgttag ataaacaaat tttcgacatg aatatattga tagtggttaa ccttctggaa
    10681 aaaaaacaac ctgatctcct acactatcta tagagccgct cggatgttgg tcaccacaaa
    10741 agcggtttct ccaggacctg gtgttaactg attggcagcg tatcgggagc ggtagccccg
    10801 gggtggaact ccgcgggcag gtcgccgggg aggaggtatg ataagcaccg tcgcattatt
    10861 ttgggcttta tgtgtcgttt gcattgttaa catggcgcgc tatttctcat cactacgcgc
    10921 gttgttagtg gtactgcgta actgcgatcc attgctctat caatatgttg atggaggggg
    10981 cttttttacc tcacatggcc aacccaacaa acaggtgcgt ctcgtttggt atatctatgc
    11041 ccaacgttat cgcgatcatc acgatgatga gtttattcgc cgctgtgagc gggtgcgtcg
    11101 gcagtttatt ctgaccagcg cattgtgtgg tctggtggtg gtcagcctga ttgcattgat
    11161 gatttggcat taagcacttc agcaataata acgcataaaa aaagcgggcc agtaaactga
    11221 cccgctcgtt ttggtgtgta cgattacagg aactgcaagg agagccagta cagcccgccg
    11281 gaaagcagta ccgcagccgg aagggtaaac acccaggcca tcagaatact ggttacggtt
    11341 ttacgctgta agccgccgcc atctaccacc atcgtccccg cgacagaaga ggagagtacg
    11401 tgagtggtgg aaaccggcat cccggtataa ctcgccaggc cgatagacac tgccgccgtc
    11461 atctgggcag acatcccctg agcgtaggtc atgcctttct taccgatttt ctcaccgata
    11521 gtcgttgcca cacgacgcca gccaatcatc gtaccgatac ctaacgccag cgcgaccgcc
    11581 atgatgatcc acaccggcgc atactcgatg gtgctaagca tgtcggactt cagtttcttc
    11641 aacaggcgct gatcgtcagc actcacacca ggcatcttca ccactttgtc gatagtgtca
    11701 gaaacgcaca gcataatgcg gcgcatctgg ctacgttgat caagcgacag cttgtcgtag
    11761 ctttccacat cggtagtcaa catgcctttc aggcggttga gcgcgttaat ggtattcgac
    11821 ggatggcaat gaaactccgc aggttgcgtt gcgccagctt ccggagccgg tactaactga
    11881 tcagcaccgg tagcttgttt cagcagcgca ggatgctgct caaagtaagc ttcgacgttg
    11941 ttgatggcat cacgggtacg ggtgatttcg tagccagtgg cattcatgtt caccacgaag
    12001 cctgctggtg cgacaccaat caataccaac ataaccagac caatgccttt ctgaccatcg
    12061 tttgcgccgt gcgaaaacgc cacgccgata gcggaaagga tcagcgcgat acgcgtccag
    12121 aacggcggct ttttcttgcc gtctttcttt tcacgctccg ctggggtcag gtggatacgg
    12181 gcgcgtttct tggtgccgct ccagtagcga cgcagcaaga aaatcagacc gccagcaaac
    12241 accaggccga caatagggga aacgatcaga gaaccgaaaa tacttaatac tttcgggata
    12301 ttgagtgcat ccaccactga cgtcccggtc atcaacgcat tggttaaacc aatcccgatg
    12361 atcgcgccaa tcagcgtatg agagctggat gcaggtaaac caaagtacca ggtacccagg
    12421 ttccagataa tcgccgccag caacatagag aacaccatgg caaggccatg agacgatccc
    12481 atattaagca gcagatccgt cggcagcata tgcacaatag cataggcaac actcagacca
    12541 cccagcaaaa cacccaaaaa gttgaacacc gctgccataa ccacggcgag ctgagaacgc
    12601 atcgcgcggg tatagataac ggttgccacg gcgttggctg tgtcatggaa accattgatg
    12661 gcttcgtaga acagcacaaa agccagtgca agcaataata acagcccggt atgcaaatcc
    12721 aggccagcaa acaaatgtag cataggacgt tacgccattt tgaggacatg aacgcggcgc
    12781 attatcagtg actttcgcgg cgcgggcaaa gtgaaatata gacttttttt gatttgcctc
    12841 ctgtatggat ttcactcaaa aaataattat cttatataat tcaggcaaat acttcctttt
    12901 agtaatattg atgctggtgc gaccactgag gaatctttac aattcacgcc cgttttttct
    12961 aagaggagcg caacgtggaa aggtttgatg ccattattat aggcgctggt gcggcgggta
    13021 tgttctgttc tgcgctggca ggtcaggcag gacgccgggt tctgctgatc gataatggta
    13081 aaaaaccagg gcgcaaaatc cttatgtctg gcggtgggcg ctgcaacttt accaaccttt
    13141 atgtcgaacc gggcgcttat ctgagccaga atccgcattt ttgtaagtct gcactcgcgc
    13201 gttttaccca gtgggatttc attgatctgg tcaataaaca cggcatcgcc tggcacgaga
    13261 aaacgttagg gcaactcttc tgcgatgact ccgctcagca gattgtcgac atgctggtgg
    13321 atgagtgcga gaagggcaat gtgaccttca gattgcgtag cgaagtgctg agcgtggaga
    13381 aggatgatac aggcttcacg cttgaactga acggcatgac tgtcggttgc gaaaagctgg
    13441 tcatcgcgac cggcgggctg tcaatgccgg gcctgggcgc gtcgccgttt ggttataaga
    13501 ttgccgaaca atttggcctc aacgtgctgc cgacccgcgc gggtctggtg ccattcactc
    13561 tgcataaacc gttgctcgaa gagttacagg tgctggcagg cgtggcggtg ccttccgtga
    13621 ttaccgctga aaacggcacc gttttccgag agaacttact cttcacccac cgcggcttat
    13681 ctggaccggc ggtgttgcag atttccagct actggcaacc gggtgaattt gtcagtatca
    13741 atctgctacc ggatgtggac ctcgaaacct tcctgaatga gcagcgtaac gcacatccga
    13801 atcagagcct gaaaaacaca ctggcggttc atctaccgaa gcggttggtt gaacgcttac
    13861 agcaactcgg gcaaatcccg gatgtctcgc taaaacagct caacgtgcgt gaccaacagg
    13921 cactgattag cacattgacc gactggcgcg tacaacccaa cggcactgaa ggctatcgca
    13981 ctgccgaagt gacgctcggc ggcgtggaca ctaacgaact ctcttcacgg acgatggaag
    14041 cgcgcaaagt gcctgggctg tacttcatcg gcgaagtgat ggacgtcacc ggctggctgg
    14101 ggggctataa cttccagtgg gcgtggtcga gtgcgtgggc ttgtgcgcag gatttgattg
    14161 cagcgaagtc gtcctgagct ttttcctaaa gatgaaaatt attagagtcg cattaaaaat
    14221 gagctaattt tgatagtggc tatcttgtga ttattttcta atgagcccgt gaactgaacc
    14281 ccccatgctt aatataaggt ggagggaaag gtgattgaaa actcacccag tgggctgatc
    14341 tttataaaat atgaacaggt tgaaaaaaaa cagtaacttt gctgtttttt atacttaacc
    14401 actatgcatt aatgctgcgt tatttcatga tacctaagaa aaaccagcgt tacgcaaatg
    14461 gtcaacgctg gttttatccg gtacgttgca attatttttt agcagaaccc gcttctaata
    14521 ttgaaacgat tgagaacaac gtaaagcata ccgctcccaa accgaccaga acccgcgaag
    14581 gaataaaata tcccatatcg gtctgcgcca tttccgcaag aaacgatgcc aggaaaaggc
    14641 aaaacaggca ggtgaagacg ggaatcatcg gtattctgtt ggctaacgaa caggtacgtc
    14701 tccagaccag tgccagtagc cagacttttg agaatatgct gtaacagatc attccgaggc
    14761 aaatgagaat aatgccggga gccagtcggg cgcttgcatc ggagctgact aagacgtata
    14821 tgccctgcag caccgtgatt gagccgagaa aaataaccca ataacaccac agccagtgct
    14881 ctttcgttga aaacgtatta cgtgtttgat ggacgattgt ggcaacaagg ccaattagac
    14941 aggcacagat tgcggttaac ccgagcaata cgtgaccggc gacataatgc ggagttatgt
    15001 cggcactacg taacagcgta atagaccaaa tgaaaccgag cagggtgagt aacacgggaa
    15061 cggcaatgag gcaattaccg attaatgaag aataagcctg taccggtgtt ccgtcgctct
    15121 tgctccccgc tgcgttttta ggaatgagca gaaagtgacc ggatgacgct gccactgtcg
    15181 atacacaggc ggcaatcata ccaacgccga aaataacatg gccggcgaca aactcgtctg
    15241 ccatcacatc attacctgct aacagtgccc aaccccatat catggtgata attgaccccg
    15301 catagccaat aatggggaac aatgtattgt aaaaagtatt aacgccgcgc gtgagctgcg
    15361 aaatgataat aaatgcggta gtgaataatg ccaggcatat ggctgccaga gaaatgagca
    15421 catgacccgc gacgaaatac tcgctttgcc ctgaactttc cagcacaaag ccacctaacg
    15481 cgatacaaat caagcccata attaaaggga ttaatttgaa aagccacccg atatatatgt
    15541 tcactttaaa ctcctcaggt atccctgcta atattgaaat gtgcattaat gataatttca
    15601 gcctgttaat acaatgacga gatatcatta aagacaaatg catagcataa ctatagcggg
    15661 gtttactttg cagagcgcta cttttcagcg ttcaaaaggc catgataaat aaat
//
LOCUS       DAZFKF010000108        15682 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFKF010000108 DAZFKF010000000
VERSION     DAZFKF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.108"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            68..409
                     /gene="yiaB"
                     /locus_tag="ACHKA1_004205"
     CDS             68..409
                     /gene="yiaB"
                     /locus_tag="ACHKA1_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO5681776.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFFGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            455..892
                     /gene="yiaA"
                     /locus_tag="ACHKA1_004206"
     CDS             455..892
                     /gene="yiaA"
                     /locus_tag="ACHKA1_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO5681777.1"
                     /translation="MDNKISTYSLAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(934..1929)
                     /gene="wecH"
                     /locus_tag="ACHKA1_004207"
     CDS             complement(934..1929)
                     /gene="wecH"
                     /locus_tag="ACHKA1_004207"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO5681778.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            2104..2403
                     /gene="ysaB"
                     /locus_tag="ACHKA1_004208"
     CDS             2104..2403
                     /gene="ysaB"
                     /locus_tag="ACHKA1_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO5681779.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            2498..3409
                     /gene="glyQ"
                     /locus_tag="ACHKA1_004209"
     CDS             2498..3409
                     /gene="glyQ"
                     /locus_tag="ACHKA1_004209"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO5681780.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            3419..5488
                     /gene="glyS"
                     /locus_tag="ACHKA1_004210"
     CDS             3419..5488
                     /gene="glyS"
                     /locus_tag="ACHKA1_004210"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO5681781.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            5810..5962
                     /gene="hokA"
                     /locus_tag="ACHKA1_004211"
     CDS             5810..5962
                     /gene="hokA"
                     /locus_tag="ACHKA1_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO5681782.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(5939..6010)
                     /locus_tag="ACHKA1_004212"
     CDS             complement(5939..6010)
                     /locus_tag="ACHKA1_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681783.1"
                     /translation="MRNAVRKAGITSRRRLLLFQIAG"
     gene            complement(6150..6362)
                     /gene="cspA"
                     /locus_tag="ACHKA1_004213"
     CDS             complement(6150..6362)
                     /gene="cspA"
                     /locus_tag="ACHKA1_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO5681784.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(6643..6933)
                     /gene="yiaG"
                     /locus_tag="ACHKA1_004214"
     CDS             complement(6643..6933)
                     /gene="yiaG"
                     /locus_tag="ACHKA1_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO5681785.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            7367..8077
                     /gene="yiaF"
                     /locus_tag="ACHKA1_004215"
     CDS             7367..8077
                     /gene="yiaF"
                     /locus_tag="ACHKA1_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO5681786.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(8127..9101)
                     /gene="ghrB"
                     /locus_tag="ACHKA1_004216"
     CDS             complement(8127..9101)
                     /gene="ghrB"
                     /locus_tag="ACHKA1_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO5681787.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVDQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALMLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETYHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALLGKVEKNCVNPHVAD"
     gene            complement(9205..9864)
                     /gene="yiaD"
                     /locus_tag="ACHKA1_004217"
     CDS             complement(9205..9864)
                     /gene="yiaD"
                     /locus_tag="ACHKA1_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO5681788.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            10017..12350
                     /gene="bisC"
                     /locus_tag="ACHKA1_004218"
     CDS             10017..12350
                     /gene="bisC"
                     /locus_tag="ACHKA1_004218"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO5681789.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLEMFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(12319..12759)
                     /gene="yiaC"
                     /locus_tag="ACHKA1_004219"
     CDS             complement(12319..12759)
                     /gene="yiaC"
                     /locus_tag="ACHKA1_004219"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO5681790.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRECIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(12756..13319)
                     /gene="tag"
                     /locus_tag="ACHKA1_004220"
     CDS             complement(12756..13319)
                     /gene="tag"
                     /locus_tag="ACHKA1_004220"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO5681791.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            13477..14175
                     /gene="yhjY"
                     /locus_tag="ACHKA1_004221"
     CDS             13477..14175
                     /gene="yhjY"
                     /locus_tag="ACHKA1_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO5681792.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGSWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            14404..15606
                     /gene="yhjX"
                     /locus_tag="ACHKA1_004222"
     CDS             14404..15606
                     /gene="yhjX"
                     /locus_tag="ACHKA1_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5681793.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLIISLALSTTIRQPQQ
                     KVLREAHI"
BASE COUNT         3837 a         3804 c         4034 g         4007 t
ORIGIN      
        1 tggcattttt cagttttgtc tttcgttggt tactcgtaat gtatcgctgg tagatatgga
       61 gatcgttatg aaaacctcaa aaactgtggc aaaactatta tttgttgtcg gggcgctggt
      121 ttatctggtt gggctatgga tctcatgccc attgttgagt ggaaaaggct atttttttgg
      181 cgtgttaatg acagcaactt ttggcaacta tgcatatctt cgcgcagaaa aactcgggca
      241 actggataat ttttttaccc atatttgcca gttagttgcg ttaatcacta tcggtctctt
      301 gtttatcggt gttttaaatg cacctatcaa tgcttatgaa atggtgatct accccatcgc
      361 cttttttgtc tgcttgtttg gtcaaatgcg tttgtttcgc tcggtatgag caacataaag
      421 ctcttacaca ttcaggaatg aaaggaatac tgtgatggac aacaaaatat caacctattc
      481 actggccttt agtattgtgt catggatagc tctcgttggt ggtatcgtta cctatctgtt
      541 agggctatgg aatgcagaga tgcagttaaa tgaaaaagga tattattttg ccgtactggt
      601 attaggactg ttttctgcgg cgtcttatca aaagaccgtt cgggacaagt atgaaggcat
      661 accgaccact tccatttatt atatgacctg cctgactgtc tttattatct ctgttgcatt
      721 actgatggta ggtctgtgga atgcgacatt attactcagc gaaaaaggtt tttatggact
      781 ggctttcttc ttaagcttgt ttggtgcagt agcggtgcag aaaaatattc gtgatgccgg
      841 aataaaccca ccaaaagaaa cacaggttac ccaggaagaa tacagcgaat aactcacgta
      901 agcccggtca gtccaatgtg accgggcttt tacttaactc actaatctgt ttctgtcgat
      961 tcgttgtacc agcatagaaa gtaacaaact cgctgccaac gtcgcgcaaa aaatccaaat
     1021 aatatccagt attggccaat ttttaagctc aattccccgg gtgcgcagtg catggataat
     1081 caaggcgtgg aatccgtata tacccaacga atggcgggag attaagccaa gtccgcgaat
     1141 ggtacgcgta tccagcgtgt ttttaaccag agtcaatagc gcgattgcgc agataaaaac
     1201 catcggccca cagtaaagat accaggtatc ggcaaaattt ccgcgccact gcaattcata
     1261 taatgtcccg cgagagataa taaaaactcc cgtcgcaaac agtgcggcgc tcacccacga
     1321 cagtgcttta tgctgtgtgt ccatcatccc tatagcgcgg cccaacatgc catacagaat
     1381 gtagtaaaaa gtatcgccat tgatatataa gttaattggc agccattcaa aaccgtcgat
     1441 tttctgcggc acggtgtttg ggttagcgat gataccaatc accaccatta gcaccagcaa
     1501 catttttccg ccgacgttct tcacctgaat cagcggtgaa accagataaa tcaccgcaat
     1561 cgcgaagaaa aaccataagt ggtaaaacac tggcttttgc agcaggtttt tcagcgctaa
     1621 ctccacatta atggaggtaa acagtgcaat gtagagcagc gcgattgcgc tataaaaaaa
     1681 cagacataag ccgatacgca agaaatggcg cggctgggcg ctgcgttcgc caaaaaagag
     1741 atagccggaa atcatgaaaa atagcggcac gctgacacga gaggcagagt tcagaacatt
     1801 ggcgatatcc caggtgacgg ggctaacact atgagcattg gtcacatacc aggtagtggt
     1861 gtgaatcatc accaccatta aacacgctat ccctcgcagg ttatcaatcc agtaaatttt
     1921 gggctgcatc tgtgtctctg tatctggtta aaaaaagtct gaccgataaa tcattcagtt
     1981 ggcgcactgg aataatctga gttttaacac tacagcttat agaggcttaa ggaaattcgt
     2041 aagatatcag ccactatacc gatataaata ataagactca cctgcaaacc agacggtaat
     2101 ttaatgatga tgaacgcttt ctttccggca atggcgctta tggtgctagt gggttgttct
     2161 acaccgtcac ccgtgcagaa agcacaacgg gtaaaggttg atcctctgcg ttcgttgaat
     2221 atggaagcgt tatgcaagga tcaggcggca aaacgttata acaccggcga gcaaaaaatc
     2281 gacgtcaccg ccttcgaaca gttccaggga agctatgaaa tgcgcggtta taccttccgt
     2341 aaagagcagt ttgtctgttc ttttgacgca gatggccatt ttttgcatct ttccatgcgt
     2401 taagtcctgc tttttcccgt ttcatactgt atatcttcca tccagcgggt atactgatcc
     2461 cttcctttaa atccacacgt atccagcacg aaataatatg caaaagtttg ataccaggac
     2521 cttccagggc ttgatcctga ccttacagga ttactgggct cgccagggct gcaccattgt
     2581 tcaaccattg gacatggaag tcggcgcggg aacctctcac ccaatgacct gtctgcgcgc
     2641 gctggggcca gaaccgatgg cggctgctta tgttcagcct tctcgtcgcc cgaccgatgg
     2701 tcgctacggc gaaaacccca accgtttaca gcactactat cagttccagg tggtcattaa
     2761 gccatcgccg gacaatattc aggagctgta ccttggttct ctgaaagagc tgggcatgga
     2821 cccgactatc cacgatatcc gtttcgtgga agataactgg gaaaacccga cgctgggtgc
     2881 ctggggactg ggctgggaag tgtggctaaa cggcatggaa gtgacgcagt tcacttactt
     2941 ccagcaggtt ggtggtctgg agtgtaaacc ggttaccggc gagatcacct acggtctgga
     3001 acgtctggcc atgtacattc agggcgtaga cagcgtttac gacctggtct ggagcgacgg
     3061 cccgctgggt aaaaccacct acggcgacgt gttccatcag aacgaagtgg agcagtccac
     3121 ttacaacttc gaatacgcgg acgtggactt cctgttcacc tgctttgagc agtacgagaa
     3181 agaagcgcag cagctgctgg cgctggaaaa tccgctgccg ctgccagctt acgagcgtat
     3241 tctgaaagcc gcccacagct tcaacctgct ggatgcgcgt aaagccatct ccgtcaccga
     3301 gcgtcagcgc tacattctgc gcattcgcac cctgaccaaa gcagtggcag aagcatacta
     3361 cgcttcccgt gaagccctcg gcttcccgat gtgcaacaaa gataagtaag aggcggctat
     3421 gtctgagaaa acttttctgg tggaaatcgg cactgaagag ctgccaccaa aagcactgcg
     3481 cagcctggct gagtcctttg ctgcgaactt tactgcggag ctggataacg ctggcctcgc
     3541 acacggcacc gttcaatggt ttgctgctcc gcgtcgtctg gcgctgaaag tagctaacct
     3601 ggcggaagcg caaccggatc gtgaaatcga aaaacgcggc ccggcgattg cccaggcgtt
     3661 cgacgctgaa ggcaaaccga gcaaagcggc agaaggttgg gcgcgtggtt gcggtattac
     3721 cgttgaccag gctgagcgtc tgactaccga taaaggtgaa tggctgctgt atcgcgccca
     3781 cgtgaagggc gaaagcactg aagcactgct gccgaatatg gttgcgactt cgctggcgaa
     3841 actgccgatt ccgaaactga tgcgctgggg tgcaagcgac gtgcacttcg tgcgtccggt
     3901 gcacaccgtg accctgctgc tgggcgacaa agtcattccg gcaaccattc tgggcattca
     3961 gtccgatcgc gtgattcgcg gccaccgctt tatgggcgag ccggaattca ccatcgataa
     4021 cgccgatcag tatccggaaa ttctgcgtga gcgcgggaaa gtcatcgccg attacgaaga
     4081 acgtaaggcg aagattaaag ccgatgccga agaagcagcg cgtaagattg gcggtaacgc
     4141 tgacttaagc gaaagcctgc tggaagaagt ggcttcgctg gtggagtggc cggtcgttct
     4201 aaccgcgaaa tttgaagaga aattcctcgc ggtgccgtct gaagcactgg tttacaccat
     4261 gaaaggtgac cagaagtact tcccggtgta tgcgaacgac ggcaaactgc tgccgaactt
     4321 tatcttcgtt gccaacatcg aatcgaaaga tccgcagcaa attatctctg gtaacgagaa
     4381 agtcgttcgt ccacgtctgg ctgatgccga gttcttcttc aacaccgacc gtaaaaaacg
     4441 tctggaagat aacctgccgc gcctgcaaac cgtgctgttc cagcaacagc tgggtacgct
     4501 gcgtgacaag actgaccgca tccaggcgct ggctggctgg atcgccgaac agattggcgc
     4561 tgacgttaac cacgcaaccc gtgcgggcct gctgtctaag tgtgacctga tgaccaacat
     4621 ggtcttcgag ttcaccgaca cccagggcgt tatggggatg cactacgcgc gtcacgatgg
     4681 cgaagcggaa gacgtagccg tcgcgctgaa tgagcagtat cagccgcgct ttgccggtga
     4741 tgacctgccg tccaacccgg tagcttgtgc gctggcgatt gctgataaga tggatactct
     4801 ggcgggtatc ttcggtatcg gtcagcatcc gaaaggcgac aaagacccgt ttgcgctgcg
     4861 tcgtgccgca cttggcgtgc tgcgaattat cgttgagaag aacctcaacc ttgatctgca
     4921 aacgctgacc gaagaagcgg tgcgtctgta tggcgataag ctgactaatg ccaacgtagt
     4981 tgatgatgtt atcgacttta tgctcggtcg cttccgcgcc tggtatcagg acgaaggtta
     5041 taccgttgac accatccagg cggtactggc gcgtcgtccg actcgtccgg ctgatttcga
     5101 tgcccgcatg aaagcggtat cgcacttccg taccctggaa gcagctgcag cactggcggc
     5161 ggcgaacaaa cgtgtatcta acatcctggc gaaatctgac gaagtgctga gcgatcgcgt
     5221 gaatgcctct actctgaaag agccggaaga aattaaactg gcgatgcagg ttgtggtgct
     5281 acgtgacaag ctggagccgt actttgcgga aggtcgttac caggatgcgc tggtcgaact
     5341 ggcagagctg cgtgaaccgg tcgatgcctt cttcgataaa gtgatggtca tggttgatga
     5401 caaagaattg cgtatcaacc gtctgaccat gctggagaaa ctgcgcgaac tgttcctgcg
     5461 cgttgcggat atttcgctgt tgcagtaata acgccgttat taaaaagcct gccatctggc
     5521 aggctttttt tatcgctaaa taatacagca acctttaata atcttctgct gaataaagat
     5581 tatctcatat attaatttta tgagattttt taggattata tcaaggagaa gaaacaaact
     5641 tattaagcta gaatagccac gggtgcttga gactgtttgt ctcaggtatt caccgaaagg
     5701 cagacagaga aaagccccac ctgactataa atcaaagtga ggcccctcta tgcatagaca
     5761 ccataaggtt agcctcttac tcgttggaaa tcaacacagg gggctgggaa tgccgcagaa
     5821 atatagattg ctttctttaa tagtgatttg tttcacgctt ttatttttca cctggatgat
     5881 aagagattca ctgtgtgaat tgcatattaa acaggggagt tatgagctgg cggcattttt
     5941 agcctgcaat ttgaaagagt aagagtcttc ggcgggaagt tattcccgcc tttcttacgg
     6001 cgttgcgcat tctcattgca cccaaattta tttttcacaa aaataatact agattttatt
     6061 acgcgatcga ttatttattt cctgaaaaac aaataaaaaa atccccgcca aatggcaggg
     6121 atcttagatt ctgtgctttt aagcagagat tacaggctgg ttacgttacc agctgccggg
     6181 cctttagcgc cgctttcgat ggtgaaggac actttctgac cttcgtccag agatttgtaa
     6241 ccatcgttct ggatagcaga gaagtgtacg aacacgtctt tagagccatc gtcaggagtg
     6301 atgaagccga agcctttgtc agcgttgaac cattttacga taccagtcat tttaccggac
     6361 atagtgtatt acctttaata attaagtgtg cctttcggcg atatggcgtg ctttacagat
     6421 tttgaagcgt taaaggaatg tgcactacga ggggtatcaa cgataactct tgaagggact
     6481 tgccttacta cactgcttta atggtctgta cgtcaaaccg ttgatgtgca ttaagccacg
     6541 cattggcggg tgatgcaaca attatttttc atatttatga ttaatcggtt ggaaaacggt
     6601 cctgtcatca ggaccgtaaa ccgcaataaa gtggataaaa gtctattcca tcaactgctt
     6661 acttaatgcc gggttggctt gaatcaaacg catcaatttt agttcggcac ttgaaggctt
     6721 cacgcgtctg gattcccatt cctttaccat ggcgactgat acgcccaaaa cccgggcgaa
     6781 atcatcgatt tttaatcctg tcccttttcg taactgctca atttcggtac aggacgttgt
     6841 tctgtgcgtc agggtaattt tctgcgtttc atctttaaaa acaatctgtt ccaggctgct
     6901 caacagctca tgcattggat ctttatattc cattgaaaac tcctcaaatc acactgcggg
     6961 atcgtgaatt gcatcgaagc tcattaagaa tagtcgggaa aagcagacac agcggggtga
     7021 agtcagggca tgctctggct atgattcgat tcagccagtc atttagcgtg acttatagcc
     7081 acctaacgat ctgataaatg ccgatatatc tcttaccact cctggtttgt aaagatttcg
     7141 aaaatctaac cttctgcgaa ggcgtaaatc tctggctaca aaaaaggcaa cggcaatatc
     7201 attttttaac aatgaaacaa caaattaacc ttgtttcatt ttctccatga ttttaccagg
     7261 aagattacgt agaaagggta tcctgcaagc ctgtcctgga ctatccttgt cacgtcagac
     7321 acgcgtgtgt cgttgtgcgc tttttttggg tgaaaggagt aagaaaatgg cgacaggaaa
     7381 gtcctgctct cgctggtttg cgcctcttgc ggcgttatta atggtagtta gcctgagtgg
     7441 gtgttttgat aaagaaggcg atcagcgtaa agcgtttatc gacttcctgc agaatacagt
     7501 gatgcgtagc ggtgaacgtc taccaaccct gactgccgat cagaaaaaac agtttggtcc
     7561 ttttgtctct gattacgcga ttctgtatgg ttattctcag caggtgaatc aggcgatgga
     7621 ttccggtctg cgcccggttg tagacagcgt taacgcaatt cgcgtaccgc aggattacgt
     7681 tacgcaaagc ggtccgctgc gtgaaatgaa tggttcgctg ggtgtgctgg cgcaacaact
     7741 gcaaaatgcg aagttgcagg ccgatgctgc gcactctgcg ctaaaacaga gtgatgacct
     7801 gaaaccggtc tttgatcagg cattcaccaa agtggtaacg acgccagctg atgcattgca
     7861 accattgatt ccggcggcac aaactttcac gcaacaactg gtgatggttg gagactacat
     7921 tgcccaacaa ggtactcagg tgagctttgt agcaaatggc atccagttcc cgacttcaca
     7981 gcaggcaagt gaatataaca aactgattgc gccattaccg gcacagcatc aggcgtttaa
     8041 tcaggcctgg actacagcag ttactgcaac ccaataaaga gtaaaagccc gagtgattat
     8101 cgggctttac tccacgcagt cgcggcttag tccgcgacgt gcggattcac acagttcttc
     8161 tcaacctttc ctagtaacgc atcaatcaaa ttatccacgg cacaggcggc catgccataa
     8221 cgcgtctcat gggtggcaga tccaatatgc ggtactgcga cgacgttggc cattgagagc
     8281 aacggcgaat ctacggaaag tggctcttgt tcgaagacat ccagcccggc ggcgtgaatt
     8341 tcgcctttct gcaatgcagc aatcagtgca ttttcatcaa ccaccgggcc acgtccggca
     8401 ttaatgaata tggcggagga tttcattttg gcgaattgtt ctgcgccaaa tagatgatac
     8461 gtctcatcag ttaacggcag gatcaggcaa acgaaatctg actcttgtaa cagtgtatcc
     8521 aaatcgcagt agcgggcgtt gaagcgttct tctgcttctt tatggtggcg gcgcgcgtta
     8581 tagaggatgg gcatgttgaa gccaaagtgc gcacgttgtg ccagcgccat accgatccgt
     8641 cccatcccga caatgcccag tgttttatgg tgaacgtcag tgccgtacca gtccgggcct
     8701 atgctcgcgg tccattcgcc tgcttttacc cgttctgcta cctccacaac ccgacgagcg
     8761 gtagacaaca tcagcgccat cagcgtatcg gcgacggttt ctgttaatac ggttggcgtg
     8821 tgcatgagca gaatttttcg ggcggtaagc gcatcgacat caaaattgtc atagccgacg
     8881 gagatcgttg atgtggcacg cagtttcggc attttttcca gcaatgcggc atctacattc
     8941 tcgtttgaac ccagtaaacc ttcagcttcg gcaaaaattg ctgcattttg atcgacggtt
     9001 tgtgggctga ggtttgccac ctggtgaacg gtgaaatgct cttgcaggcg ttgcagtaaa
     9061 tcatcaggta aggctttgta gaggataacg gacggcttca tgcttctctc cattcactga
     9121 taaggcgatc accttagcct gaaatatgat gaaggaaaac tgaagaatgc caggtgattt
     9181 ctgcatcacc tggcatgaaa gggattacag cgggcttaag gtaatttcta cacggcggtt
     9241 ttgcgcctta ccttctgcgg tgctgttgct ggcgattggg ttagccgggc caaggccctg
     9301 agtacggatg cggctggcgt ccacgccctg ggtgatcaac gcgctggcaa cggaatccgc
     9361 acgctgttgg gagagacgca tgttcagatc gtgaccaccc gtgctgtcgg tataaccaat
     9421 cacgttaacc gccgttttcg gatactcttt cagcaccatt gccacgccgg tcagggtgtt
     9481 agcgcccgca ggtttcaggg tcgcgctgct gctgtcgaag gtcacattgt tcggcatatt
     9541 gaggataatg ttatccccgc tgcgggttac actaacacca gtgccgcgca ttttgtcacg
     9601 cagcttcgct tcctgcacat ccatgtaata acccacgccg ccgcccagag ctgcgcctgc
     9661 tgctgcgcca atcagcgcgc ctttaccgcg atctttcttc gaagaagaga gcgcaccaat
     9721 acccgcgccc acgagagagc ccagacctgc gccgatagca gatttacctg cttcgcgttc
     9781 gccggtgtaa gggttagttg tgcagccaga taccgccaga gcgccactca ctacggcggc
     9841 aataagataa acacgtttct tcattgttaa tccttaataa cctttttatt ctttgccacg
     9901 ggttccgtgg cgggagatta tgccgcgtga acatgaagat tattcctggg aatactcgga
     9961 aatttgtaag taatatttaa ctgctcaata catctaacct ttcaggagtc ttcggtttgg
    10021 ccaactcatc ctcacgatat tccgttctga ctgccgccca ttgggggccc atgctggttg
    10081 aaaccgacgg cgaaaccgtg tttagctcgc gtggcgcgtt agccacagga atggaaaact
    10141 ccttgcagag cgcggttcgc gaccaggttc acagcaatac gcgggtacga tttccaatgg
    10201 tgcgcaaagg ctttctcgcg tcaccggaaa acccgcaagg cattcgtggg caggatgaat
    10261 ttgttcgcgt gagttgggat gaggcgctgg atcttattca ccaacaacat aaacgcattc
    10321 gtgaggctta tggtccggca tcgatttttg ctggttccta cggctggcgt tcaaacggcg
    10381 tgctgcataa ggcctcgaca ttattacaac gctatatggc gctggcaggc ggttataccg
    10441 ggcatctggg ggattattcg accggcgcgg cacaggcgat catgccgtat gtcgtgggtg
    10501 gtagtgaagt ttatcaacag cagaccagtt ggccgctggt gctggaacat agcgatgtcg
    10561 tggtgctgtg gagtgctaac ccactcaata cgctgaaaat tgcgtggaat gcatccgatg
    10621 agcaggggct ttcttacttt tctgcactgc gtgacagcgg gaaaaagctg atctgcattg
    10681 atccaatgcg atcggaaacc gtcgatttct ttggcgataa aatggagtgg gtggcaccgc
    10741 acatgggcac tgatgttgcg ctgatgctgg ggatcgccca tacgctggtg gaaaatggtt
    10801 ggcacgacga agcgtttctg gcgcgttgca ccacaggtta tgccgtcttc gcctcttatt
    10861 tgctgggcga gagtgacgga atagcgaaaa ccgccgaatg ggcagcagag atttgtggtg
    10921 ttggcgcagc gaaaatccgc gagctggcgg ctattttcca ccaaaatacc accatgctga
    10981 tggctggctg gggaatgcag cgccaacagt ttggtgagca aaaacactgg atgatcgtca
    11041 cgctggcagc aatgttgggg caaatcggca cacccggcgg cggttttggt ctttcttacc
    11101 attttgccaa tggtggtaac cccacgcggc gcgctgcggt actctcttcc atgcagggca
    11161 gcttgccggg tggctgcgat gcggtagata aaatccctgt cgcccgcatt gttgaagcac
    11221 tggaaaaccc cggtggcgca tatcaacaca acggtatgga ccgacatttc ccggatattc
    11281 gttttatctg gtgggcgggc ggtgccaact ttactcatca tcaggatacc aaccgcctga
    11341 tccgtgcctg gcaaaaaccg gagctggtgg tgatctccga atgtttctgg acggcggcgg
    11401 cgaaacacgc ggatatcgtt ctgcctgcga ctacctcttt tgagcgtaat gatctcacca
    11461 tgaccggtga ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg ccgccacgct
    11521 atgaagcgcg taatgatttt gatgtttttg ccgagttaag tgaacgctgg gagaagggcg
    11581 gctatgcgcg gtttacggaa ggaaaaagtg agctgcaatg gctggaaatg ttttataacg
    11641 ttgcccgaca gcgcggggca agccagcagg ttgaattgcc gccatttgct gagttctggc
    11701 aagccaatca gttaattgag atgccggaaa acccggacag cgagcggttt attcgcttcg
    11761 ctgatttttg ccgcgatccg caggcgcatc cattaaaaac cgccagcggt aagattgaaa
    11821 tcttctcgca gcgtattgcc gattacgctt atccggattg cccggggcat ccaatgtggc
    11881 tggagccgga cgaatggcag ggtaatgccg aaccggaaca gttacaggta ctttctgctc
    11941 atccggcgca tcgtctgcac agccagctga attacagttc tctgcgcgaa ttgtacgcgg
    12001 tagcaaaccg tgagcctgtg accattcatc ctgacgatgc tcaggcgcgc ggcatacaag
    12061 atggcgatac tgttcggctg tggaactcac gcgggcaaat tcttgccgga gcggtcatta
    12121 gcgagggaat taaacctggc gtgatttgca ttcacgaagg ggcatggccg gatctggatt
    12181 taaccgctga cggtatttgt aaaaacggcg cagtgaacgt gctgaccaaa gatctcccca
    12241 gctcgcggct ggggaatggc tgtgcgggta atacggcgct ggcatggcta gaaaaataca
    12301 acggtccgga actgacactt acagcgtttg aaccaccggc cagctcataa tccaggtggg
    12361 tagttgggtt tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg cctggtaaaa
    12421 atctatcgcc ggttgatttt tttgataaac ctccagcatc agatggggat ggcgctgttg
    12481 cacatactgc atcagcgcct taccaatacc gcgcctgacg gccttcggtg cgacaaacat
    12541 cgctgccaga aatcggcctt ccataatgct gacaaaacca agaagcttac tgtcttcttc
    12601 ccagacccag ttttgtgcgt tggcaagata ggcatcccgc accagcggaa tgcactcgcg
    12661 ccagtaattc gcttttataa agggatggcc ccaggttgta ctttccagcc acagttcgag
    12721 gatcgcgggg agttctgaac gttgcgcttc cctaatcatg gtttatttcc cggatagcaa
    12781 cagcagccaa ccacatgatc attcaccagc ccacatgcct gcataaagga gtaacagatt
    12841 gtggtaccga caaacttaaa accacgtttt ttcagtgcct tagatagggc gtcggaggcg
    12901 ggcgtagatg tggggatttc gctcaacgtt gtggcttgtg tcacctgtgg ctgatgattt
    12961 acaaacgacc agacaaagtc ggcaaacggt tcgccgttct gttccatttg caggtacgcc
    13021 cgcgcattac caataattgc ctgaattttc ccgcgatggc ggataatccc ggcgtcctgt
    13081 accagtcttt cgacatcctc ttcctgcatt gcggcaacct tcaccggatc gaactgatga
    13141 aagcaggcgc gatagttttc gcgttttttg aggacggtga tccacgataa cccagcttgc
    13201 tgcccttcca ggcagatcat ttcgaacagt tttttactgt cagtttcagg cacgccccac
    13261 tcattatcat ggtaggcaat ataaagcggg tcctgactca cccagccgca acgttccata
    13321 ctttccctcg ctatgtaccc gatcgtaaaa atttcagccg cttttcttga cgcatcggtg
    13381 aaaaagtcaa tatttaatac agggctacta tgcccgatat ccttcataca atgacaataa
    13441 tattgccgaa ttcggctctt ccctggagtc gtgttaatga tcataaaaaa aagcggtggt
    13501 cgctggcagc taagcctgct ggcgagtgtg gtaatcagtg ccttttttct caacacagct
    13561 tacgcctggc aacaagaata tatcgttgat acgcaacccg ggcattccac agagcgttac
    13621 acctgggata gtgatcatca acctgattac aacgatattt tgtcgcaacg tattcaaagt
    13681 agccaaaggg cgctgggact ggaagtcaat ctggcggaag aaacccctgt ggatgtgacc
    13741 agcagtatga gtatgggctg gaattttcct ttatatgaac aggttacaac tggcccggtc
    13801 gcggcattac attacgatgg cacaaccacc tcgatgtata acgagtttgg cgacagtact
    13861 accacgctga ccgatccgtt atggcatgcc agcgtgagta ccttaggctg gcgtgttgac
    13921 tcccggcttg gcgatctccg accctgggcg caaatcagct ataaccagca atttggcgag
    13981 aatatctgga aggcgcaatc aggcctgagc cggatgacgg cgacaaacca gaacggcagc
    14041 tggctggatg tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc cgcctatgcc
    14101 gcgctatctc aggcagaaaa taccactaac aatagcgatt atctgtatac gatgggggtt
    14161 agtgccagat tttaacgtaa caatcacaat tgaaaccatt gaataacaat agttgtggcg
    14221 atagtgggtg ctaacttacc aaataataaa tttggtgaat aattgtcgcg tcattcattc
    14281 ctgaactaag gcatttcatt ccgttctgat ggcatttcat gccgtttttc cccaggcata
    14341 aagtgcactt cgttatggtt gtcggcagag atttttcctt tttattactg caggaatact
    14401 gccatgacac cttcaaatta tcagcgtacc cgctggctga cactcatcgg tactatcatt
    14461 acccagtttg cgctggggtc ggtttatacc tggagcctgt ttaacggcgc gctttccgcc
    14521 aagctggatg cgccggtaag ccaggtcgct ttctctttcg gcttgttaag tctggggctg
    14581 gcaatttcgt cttctgttgc gggcaaattg caggaacgtt ttggtgttaa acgcgtcacc
    14641 atcgcttccg gcattttgct gggattaggg ttcttcctga cagcgcattc tgacaacctg
    14701 atgatgctgt ggttaagcgc cggtgtgctg gtcggtctgg cggatggcgc gggttacctg
    14761 ctgacgctct ctaactgcgt gaaatggttc ccggagcgta aaggtctgat ctccgcgttc
    14821 gctatcggtt cttatggtct gggtagcctg ggtttcaaat ttatcgacac gcagctgctg
    14881 gaaacggtcg gtctggaaaa aacctttgtg atttggggag cgattgcgct ggtgatgatt
    14941 gttttcggcg caacgttaat gaaagacgca ccaaaacagg aagtgaaaac cagcaatggt
    15001 gtggtggaga aagattacac gctggcagag tcgatgcgta aaccgcagta ctggatgtta
    15061 gcggtaatgt tcctgactgc ctgcatgagc ggcctgtacg tgattggggt agcgaaagat
    15121 atcgcccaaa gtctggcaca ccttgatgtg gtttctgcag ccaatgcagt cactgttatt
    15181 tccatcgcca acctttcagg tcgtctggtg ctgggcattc tgtctgacaa aatcgcccgt
    15241 atccgtgtta tcaccattgg tcaggtgatt tcgctggtgg gtatggcggc cctgctgttt
    15301 gcaccattga atgcagtgac gttctttgca gcgattgcct gtgtggcatt taactttggc
    15361 ggcactatta ccgtctttcc gtcactggtc agtgagttct tcggcctcaa taacctggcg
    15421 aaaaactacg gtgtgattta cctcggtttc ggtatcggta gcatttgtgg ttcgattatc
    15481 gcctcactgt ttggcggctt ctatgtgacg ttctacgtca tctttgctct gctgattatt
    15541 tccctggcac tttccaccac cattcgccag ccgcagcaga aagtgttgcg tgaggcgcat
    15601 atttaatgag atggcgttgg ttattaacga aggggcgaga gagccccttt ttttcgttta
    15661 aaataataag gagattacaa aa
//
LOCUS       DAZFKF010000109        15622 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFKF010000109 DAZFKF010000000
VERSION     DAZFKF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.109"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            62..628
                     /gene="yceJ"
                     /locus_tag="ACHKA1_004223"
     CDS             62..628
                     /gene="yceJ"
                     /locus_tag="ACHKA1_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO5681794.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            632..1207
                     /gene="yceI"
                     /locus_tag="ACHKA1_004224"
     CDS             632..1207
                     /gene="yceI"
                     /locus_tag="ACHKA1_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO5681795.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1249..2301)
                     /gene="trhO"
                     /locus_tag="ACHKA1_004225"
     CDS             complement(1249..2301)
                     /gene="trhO"
                     /locus_tag="ACHKA1_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO5681796.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPACAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2526..3446
                     /gene="lpxL"
                     /locus_tag="ACHKA1_004226"
     CDS             2526..3446
                     /gene="lpxL"
                     /locus_tag="ACHKA1_004226"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO5681797.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3618..4844
                     /gene="mdtG"
                     /locus_tag="ACHKA1_004227"
     CDS             3618..4844
                     /gene="mdtG"
                     /locus_tag="ACHKA1_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO5681798.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4927..5301
                     /gene="msyB"
                     /locus_tag="ACHKA1_004228"
     CDS             4927..5301
                     /gene="msyB"
                     /locus_tag="ACHKA1_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO5681799.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5302..5529)
                     /gene="yceK"
                     /locus_tag="ACHKA1_004229"
     CDS             complement(5302..5529)
                     /gene="yceK"
                     /locus_tag="ACHKA1_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO5681800.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5702..8245)
                     /gene="mdoH"
                     /locus_tag="ACHKA1_004230"
     CDS             complement(5702..8245)
                     /gene="mdoH"
                     /locus_tag="ACHKA1_004230"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO5681801.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPLDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8238..9773)
                     /gene="mdoG"
                     /locus_tag="ACHKA1_004231"
     CDS             complement(8238..9773)
                     /gene="mdoG"
                     /locus_tag="ACHKA1_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO5681802.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPSNSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10166..11323
                     /gene="mdoC"
                     /locus_tag="ACHKA1_004232"
     CDS             10166..11323
                     /gene="mdoC"
                     /locus_tag="ACHKA1_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO5681803.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSLIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11331..12812)
                     /gene="clsC"
                     /locus_tag="ACHKA1_004233"
     CDS             complement(11331..12812)
                     /gene="clsC"
                     /locus_tag="ACHKA1_004233"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO5681804.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12754..13287)
                     /gene="ymdB"
                     /locus_tag="ACHKA1_004234"
     CDS             complement(12754..13287)
                     /gene="ymdB"
                     /locus_tag="ACHKA1_004234"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO5681805.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENSHLYERLLTQQGDE"
     gene            complement(13382..13693)
                     /gene="ymdA"
                     /locus_tag="ACHKA1_004235"
     CDS             complement(13382..13693)
                     /gene="ymdA"
                     /locus_tag="ACHKA1_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO5681806.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNSQVASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13814..14146)
                     /gene="csgC"
                     /locus_tag="ACHKA1_004236"
     CDS             complement(13814..14146)
                     /gene="csgC"
                     /locus_tag="ACHKA1_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO5681807.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14205..14660)
                     /gene="csgA"
                     /locus_tag="ACHKA1_004237"
     CDS             complement(14205..14660)
                     /gene="csgA"
                     /locus_tag="ACHKA1_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO5681808.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14701..15156)
                     /gene="csgB"
                     /locus_tag="ACHKA1_004238"
     CDS             complement(14701..15156)
                     /gene="csgB"
                     /locus_tag="ACHKA1_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO5681809.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3895 a         3991 c         3706 g         4030 t
ORIGIN      
        1 ttgcaattca ttctccgaca cgccattttc tttctctcgc aaattcgttt tggatgcact
       61 tatgtcattc acaaataccc ctgaacgcta tggtgttata tctgcggcct tccattggct
      121 aagcgcaatt attgtctacg gcatgtttgc tttaggtctg tggatggtca cgctcagtta
      181 ttacgatggc tggtatcaca aagcacccga actgcataaa agcatcggta ttttgttaat
      241 gatggggctg gttattcgcg ttctgtggcg cgtcatatct ccaccgccgg gtccgctgcc
      301 gagttattcg cccatgactc gtcttgccgc aaaagctgga catctcgcat tgtacctatt
      361 gctgttcgcc atcggcatca gcggctatct gatctcaact gccgatggta aaccgatcag
      421 cgtttttggc tggtttgacg tccccgcgac ccttgccgac gctggcgcac aggcagactt
      481 tgccggtgcc ctgcattttt ggctcgcgtg gagcgtcgta gtattgtccg ttatgcacgg
      541 atttatggcc ctgaagcatc atttcatcga taaagacgac actctgaagc gcatgctggg
      601 aaagtcgtca tctgactatg gagtataaaa aatgaaaaaa agcctgcttg gtttaacctt
      661 cgcgtccctg atgttctctg ccggttcagc ggttgccgcc gattacaaaa ttgacaaaga
      721 aggtcagcac gcctttgtta acttccgcat ccagcatctt ggctatagct ggttatacgg
      781 cacctttaaa gatttcgacg gtacttttac ctttgatgaa aaaaatccgg ctgccgataa
      841 agtgaatgtg acaattaaca ccaccagcgt cgatactaac cacgccgaac gcgataaaca
      901 tcttcgcagt gcggattttc tcaataccgc aaaatatcca caggcaacat tcacctccac
      961 cagcgtgaag aaagacggtg acgaactgga tattaccggc gatctgacgc tgaatggtgt
     1021 aaccaaaccc gtcacgctgg aagcgaaatt aattggtcag ggcgacgacc catggggtgg
     1081 taaacgtgca ggcttcgagg ccgaaggcaa aattaagctc aaggacttca atatcaagac
     1141 agatttaggt ccagcttctc aggaagtgga tctgattatt tcagtggaag gcgtacagca
     1201 gaagtaagag tgaaatgttg ccggatggca cgcatccggc aatgatattt attctgttgg
     1261 atcaggaatg cccagtgttg tattcagacg tccacgagac ttattaaaga tcttattgcc
     1321 attttcacgt cctgcccgac ggcgtcgctg ttcttctggc ggtaacgcgc tttcttcgca
     1381 gcaaatctca ctacaacaac ctttgtattt ttccgcacat gctggacact gaataaacag
     1441 caggtggcag ccatcatttt tacagttggt atggctgtcg cacggcgcac cgcactggtg
     1501 gcaatgcgcg ataatctcat ccgatatacg ttcgcccatc cgctcgtcaa aaacaaaatt
     1561 tttgccaata aaacgcaccg gcaagccctg ctcgcgcgcc ttacgggcgt attcaataat
     1621 tccgccctcg atatgccaga ctttgttgaa tccgttatgt ttcatccagg cactggcctt
     1681 ttcacaacga atgccgccgg tgcagtacat gacgattttt ttatctttat gtgcctgcat
     1741 catctcgact gcttttggca gctgctcacg gaaggtatct gccggaattt cgagcgcgtt
     1801 ttcaaagtgc cccacttcat actcatagtg gttacgcatg tcgataaata gtgcatcggg
     1861 atcgtcaagc atggcgttca cttccgccgc ttgcagatac tcaccaacat tgctggcatc
     1921 aaagtgagga tcgtcaatac cgtcggcaac gatacgatcg cggaccttca tgcgcagtac
     1981 ccagaaagat ttcccatcat catccaacgc gatattcagg cgtaaaccct ctaaagccgg
     2041 gtcgaaggca tagagctgcg cgcgaaatgt ttcaacattg ctcgctggta cgctgatttg
     2101 cgcgttaatg ccctcatgcg ccagatacac tcgcccaaaa acattcagcg cggtaaacag
     2161 ctgatataaa gcgtcacggg tcgccttagg atcggcgatg tggaaatact tgtaaaacga
     2221 aatggtggtt cgcggttcgc tctcagccaa cattttggct tttaacgcgt cgttggaaat
     2281 gcggttgtgt aacactggca tggtgtacgg ttcctgcgag atgggaaagt aaaaatccgc
     2341 ggcatgatat agcaattatc gataattaac atccacacat tttacgctac atttgcgcat
     2401 taaaaattat ttgttattta caagcgcggc aatttcgccc agtcttcagc cacaaatttt
     2461 ggttgcgggc gaaaaaatgc gacaatacat acaattgccc gaataggttg aaaaacagga
     2521 ttgatatgac gaatctaccc aagttctcca ccgcactgct tcatccgcgt tattggttaa
     2581 cctggttggg tattggcgta ctttggttag tcgtgcaatt gccctacccg gttatctacc
     2641 gcctcggttg tggattagga aaactggcgt tacgttttat gaaacgacgc gcaaaaattg
     2701 tgcatcgcaa cctggaactg tgcttcccgg aaatgagcga acaagaacgc cgtaaaatgg
     2761 tggtgaagaa tttcgaatcc gttggcatgg gactgatgga aactggcatg gcgtggttct
     2821 ggccggaccg ccgaatcgcc cgctggacgg aagtgatcgg catggaacac attcgtgacg
     2881 tgcaggcgca aaaacgcggc atcctgttag ttggcatcca ttttctgaca ctggagctgg
     2941 gtgcgcggca gtttggtatg caggaaccgg ggattggcgt ttaccgcccg aacgataatc
     3001 cactgattga ctggctacaa acctggggcc gtttgcgctc aaataaatcg atgctcgacc
     3061 gcaaagattt aaaaggcatg attaaagccc tgaaaaaagg cgaagtggtc tggtacgcac
     3121 cggatcatga ttacggcccg cgctcaagcg ttttcgtccc tttatttgcc gttgagcagg
     3181 ctgcgaccac gaccggaacc tggatgctgg cacggatgtc cggcgcatgt ctggtgccct
     3241 tcgttccacg ccgtaagcca gatggcaaag ggtatcaatt gattatgctg ccgccagagt
     3301 gttctccgcc gctggatgat gccgaaacta ccgccgcgtg gatgaacaaa gtggtcgaaa
     3361 aatgcatcat gatggcacca gagcagtata tgtggttaca ccgtcgcttt aaaacacgcc
     3421 cggaaggcgt tccttcacgc tattaaatct cccatgccgg atgcttcaga atggcatccg
     3481 gcattaccac agcaaatccc cctgatttag cgataaaagc tctctggatt gcgccccctg
     3541 gaagtcgggc gcataattag tgtgcttatc ttttcttctt atgttcaccg cgcctggcgc
     3601 accaacagcg gattgctatg tcaccctgtg aaaatgacac ccctataaac tggaaacgaa
     3661 acctgatcgt cgcctggcta ggctgttttc ttaccggtgc cgccttcagt ctggtaatgc
     3721 ccttcttacc cctctacgtt gagcagcttg gcgttaccgg ccactccgcc ctgaatatgt
     3781 ggtccggtat tgtcttcagc attacatttt tattttcggc catcgcctca ccgttttggg
     3841 gtggactcgc cgaccgtaaa ggccgaaaac tcatgctatt acgctctgct ctcggcatgg
     3901 gcatcgtgat ggtgttgatg gggctggcac aaaatatctg gcagtttttg atccttcggg
     3961 cgcttcttgg gttacttggc ggatttgtcc ccaacgctaa tgctcttatc gccacacaag
     4021 taccgcgtaa taaaagcggc tgggcactgg gtacgctctc cacaggcggc gttagcggtg
     4081 cgttgctcgg cccaatggct ggcggcctgc tcgccgatag ctacggctta cgtccggtat
     4141 tctttattac cgccagtgtg ctcatactct gctttttcgt caccctgttc tgcatcagag
     4201 aaaaattcca gccggtcagc aaaaaagaga tgctgcacat gcgggaagtg gtgacatcac
     4261 ttaaaaaccc gaaactggta ctcagcctgt ttgtcactac gttaatcatc caagtggcga
     4321 cgggctcaat tgcccccatt ctgacgctgt atgtccgcga actggcgggt aacgtcagta
     4381 acgtcgcctt tatcagtggc atgatcgcct cggtgccagg cgtggcggct ctgctgagtg
     4441 caccacgact cggcaaactt ggcgatcgaa tcggccccga aaagatcctg attacggcgc
     4501 tgatcttctc tgtactgctg ttgatcccaa tgtcatacgt tcagacgcca ttacaacttg
     4561 ggattttacg ttttttgctc ggtgccgccg atggtgcact actccccgcc gtacagacac
     4621 tgttggttta caactcgagc aaccaaatcg ccggacgtat cttcagctat aaccaatcgt
     4681 ttcgtgatat tggcaacgtt accggaccat tgatgggagc agcgatttca gcgaactacg
     4741 gtttcagagc ggtatttctc gtcaccgctg gcgtagtgtt attcaacgca gtctattcat
     4801 ggaacagtct acgtcgtcgt cgaatacccc aggtatcgaa ctgatttttc gcctttcata
     4861 cttgcaaaag cggagaatca gctatccttt tccctgaaac ctcatcaact caaagggaga
     4921 atcgtgatga ccatgtacgc aacgcttgaa gaagccattg acgctgcacg cgaagaattt
     4981 cttgcagaca accccggcat cgacgccgaa gacgcgaatg tgcaacagtt caatgcccaa
     5041 aaatacgttt tgcaggacgg cgacatcatg tggcaagttg agttttttgc cgacgaaggg
     5101 gaagaaggtg aatgtttacc tatgcttagc ggtgaagccg cgcaaagtgt ttttgatggc
     5161 gactatgatg agatagagat ccgccaggag tggcaggaag agaatacatt acatgaatgg
     5221 gacgaggggg aatttcagct tgagccaccg ctggataccg aggaaggacg cgcagcagct
     5281 gatgagtggg atgaacgtta atcactcata cgggccatga tgaatgtcga tcggcagcag
     5341 taaagtatca aaaaccaggg agaatggcag atcaaggatt gtgacataac gccaggcgga
     5401 gtcacgcaca tcccattgca caccgggata atattggttg ccatgcccct gccccggtat
     5461 agtgcggcta atgatgctgc cacagccact cagcaaggtc accatgatgc tcaccacaat
     5521 taaacgcatt tgatacctct tccgtaaaaa aataccggca taacgccggt atttttattg
     5581 gctcgttgtt tttgccagat gcgacgtgaa cgccttacct aacctacaaa atcttgcaaa
     5641 atcaataaat tgcaggaacg atgtaggcct gataagcgta gcgcatcagg caactacgtt
     5701 tttattgcga agccgcatcc ggtttacgca atgccagtgg attgagcttt atcccttcgt
     5761 aataactcac ccatgaagaa tatctctcag gggaattcca gacgcggaaa tgcagacggg
     5821 ccatcgtcac cggatcgctt agcagcacaa ggcgacgatc gcgattcagc ttctctggcg
     5881 tctcgttcag cgcctgttca acgtggcggt cacgggcgat ttccagcacc ttactggcgc
     5941 ggtgacgcgc ggtcgccatt gcggtggcca gagcgttaaa tgacgggtta aacactgcgt
     6001 gcataaaacc atcatcaagg gagcgttgac ggttcatctc aaggaaccga tcggtatcaa
     6061 ccagtacctg cggcggtgaa tactcttccg ggatcaggaa cagtttccag cgtttggtgc
     6121 gcagaccaac ggtggcacgg ctggaaatca ccgaaacaaa cggtgacagg atcaacgaga
     6181 agacaatcgg tgccagccag aacaggaaac gcagatccaa ccacgccatc ccaacagccc
     6241 acactaaccc taacagcagc tgtgagccgt ggcgtttgaa cgcttcaccc caggaagtgg
     6301 agtcatcatc acgctgcggt gaattccaca ccacttccca gccaaggaac gcgctgacga
     6361 cgaagaccgt atggaacagc atgcgtaccg gagccagcag cacggaaaac agcacttcca
     6421 gcagcaacga taatgtaacg cgccagaagc cgccatattc tttcgttcct ttgcaccaaa
     6481 taagcaaaat gctcaatagc tttggcagga acaacagcac catggtcgaa gcaaaaagtg
     6541 caatcgccag ctcaggacgc cactgcggcc acaccgggaa caactgccgt ggttgcagga
     6601 agtattgcgg ttcggttaac gcatgcacta cctgcaatgc agtagagagc gcgaggaaca
     6661 taaaccacag cggagcggag agataagaca tcacgcccgt caggaacacc gcacggtgaa
     6721 ccgggtgcat acccttaacc aggaacagac ggaagttcat caggttaccg tggcaccagc
     6781 ggcggtcacg ttttagctca tcaagtaagt taggcggtaa ttcttcataa gaacctggga
     6841 gatcgtaagc aatccagact ccccaacctg cacggcgcat caacgccgct tccacgaagt
     6901 catgtgacag gattgaaccg gcaaaggaac cttcgcccgg cagcggagcc agtgcgcagt
     6961 gctcgataaa cggtttcacg cggataatcg cgttatgtcc ccagtagtgc gactcgccaa
     7021 gttgccagaa gtgcaaaccg gctgtaaaca gtggcccata cacgcgggtc gcgaactgct
     7081 gacagcgcgc atacagcgta tccataccgg acgctttcgg cgacgactga atgatcccgg
     7141 cgttcgggtt ggcttccatc aagcgcacca ggccgcataa acaatcaccg gtcattaccg
     7201 agtcagcatc cagcaccacc atgtagctgt actggctgcc ccagcgacgg cagaagtcat
     7261 cgatattacc gcttttacgc ttcacgcgac gacggcggcg gcgatagaaa atctgacctt
     7321 cgccaccgac ttcagcgata agctccatcc aggctttttg ctctgcgacg cagatatccg
     7381 ggttataact gtcactcaga atgtagacat caaagtgttt ggcattcccg gtggctttta
     7441 ctgattccca cgttgcacgc aggccagcaa aaacacggtt cacgtcttcg ttacagatag
     7501 gcatgatcaa cgccgtgcga tgctccgggt ttaatggttc atcgccaact gttgacgcag
     7561 atatactgta tttatcgcga ccaataagca gttgcaggaa gcccattaac gccgtccaga
     7621 atccggcgga cacccaacag aacagtaccg caaagaggat caggataccg gtttgcagca
     7681 tataaggcag aagctgcata aaggaaaccc acaaatcctg accaaccata tccaaaggat
     7741 taatcagcgc ccaaccctga taaggaagaa tggtcttcat ataccaggtc gcgacgacag
     7801 tttgcgcgag cgtcaggatc aacagaatgt aacggcggat ggtaccgacg gtacgccact
     7861 tttgctcgct ctcctgctct tctttggtca aacgagccag atagcgcggc gtgacatcgc
     7921 gtccacgcag gcgatcccag aaacggccta ccgggttggt acgccacggg tcgggaaaca
     7981 tcgaggagcg ttttgcttct ggcatcgcct taagctgatc gcgcccttcg tcgtctttaa
     8041 ttaactgtcc atcagcaagt gaatctggcc aggcttgttc cagacgcgcc tttaccgagc
     8101 cttgcgggga gtcatcctcc cgcgcccagg tgcggtgttc ggcatccagc gcctgatgaa
     8161 cggcgcggat atcagtcttc ggcaatgccg ctttctcgct tgcggcgatg ggcattgcgt
     8221 caatgtactc agttgtctta ttcattggca ggtaactggt agctccaggt ttcactcaac
     8281 gtctgatcgg cattcaccag cgcagctcgc atttcagtgg ttttcttggc atctttcact
     8341 ttcacacgca tcaccagacg ccagcctttg gtaaccgggt tatagcgcac cgtgctttca
     8401 actatctcac cattatcacc aatgctggtt tgcgctgtga ccggggtatc ctctggcagt
     8461 tttttcatct ctgcgccggt aaaatcgacc acaaaggcga tagtaccgtc aggctggcgg
     8521 atcaggttcg actgcttcac atcccccgtt gaacgacgcg tttgttgcac ccatgcgtta
     8581 tctggtgcat gcagtttgtc ttcatcacgg ctgaaggtga tggtgtattt aaagttcatc
     8641 tctttacccg gctccggcag ctgatccggc gtccagtaag cgacgatgtt atcgttagtt
     8701 tcatcgttgg ttggaatttc caccagctca acgctgcctt tgccccactc ccctttcgga
     8761 gtcacccatg cgcttggacg gagatcgtaa cgatcatcga gatcttcaaa gcgggagaaa
     8821 tcacgaccgc gctgcaacag accgaagcct tgcgggtttt ccatggagaa gctgctgacc
     8881 gctaaatgtt tcgggttatt caacggacgc cagatccatt cgccattacc ggcatggata
     8941 gagagaccgt tagagtcgtg caactccgga cgatagttat ttgcaggcga cggttggttc
     9001 ggcccaaaca ggaacatact ggttaacggt gcaaccccca gtttgccgac tttatcgcgc
     9061 agatagattt tcgactgcac atccacaacc gtgtcacgcc ctggcataac tacgaatttg
     9121 taagcacctg tcgcgcgcgg cgagtcaagc aatgcataaa tggttaaacg tttatcagtt
     9181 ggttttggac gctcgatcca gaactctttg aagcgtggaa attcttcacc cgatggcaag
     9241 gcggtatcaa ttgccagacc gcgggcagaa aggccataaa cctgacctgc accaatcacg
     9301 cggaaatagc tggccccgag catgctgacg atttcatcgt ttttatcttt gctgttgctc
     9361 gggtaaagca ctttaaaacc ggcaaaacca aggtctttta ccgtgtcttt gtcatgctga
     9421 acatcgccga aagtgaaata atccgggctg tatttgattc gtttgactgc ggtggcagtc
     9481 acttcattta ttttgaccgg ggtatcgaag tacatcccct gatggtagaa ctcgagtttg
     9541 aatggggtct tcagattgtt ccagtacgct ttgtcatgat taaactggat ctgctgatag
     9601 tccgcgtatt tcatatcgcg gaaaacggag ggcaagttgc ttttgggcgc ctcatagcct
     9661 ttcccggcta aggattgagc ttgctttgcg acatcatcaa tactgaaagc ccagcttgaa
     9721 gatgtataca gggttaacat tactgcagca ctcaaccaac gcattttcat catttgtagt
     9781 ttatgtttca taattagtaa gcacttcccc ctttgtgtgc ttatatcgat ccgatctatt
     9841 ttaatggata accagccatt ccgacaacag aatcccggta tcgttcagca cgctggctca
     9901 tggattaagc acctgattgc aaccgttttc actttccgtg cttctcctaa agacagaagc
     9961 atcgacttcg tgtagggttt gccagcaaat ttatttatca accgtcttac agacgcttcc
    10021 attctggtta gtggtagcga tgatgctatg cataaaaact tgaagcctgc gcctggcgcg
    10081 gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata acccgacagg gataagacga
    10141 aaaatccgag attagttaac catatatgaa tccagtaccc gcgcaacgtg aatatttcct
    10201 cgactccatc cgcgcctggc tgatgttgtt agggatccct tttcatattt ctttaatcta
    10261 ttcgagccat acatggcatg tgaatagcgc cgaaccgtca ttgtggctga ccctttttaa
    10321 tgacttcatc cactcgttcc gcatgcaggt atttttcgtt atatccggct acttttccta
    10381 catgcttttt ttacgctatc ccttgaaaaa atggtggaaa gtacgtgtcg aacgtgtagg
    10441 tatcccgatg ttaacagcca tccccctact gacattgccg caatttatta tgctgcaata
    10501 cgtcaaaggg aaagcggaaa gttggcctgg gctgtcattg tatgacaaat ataatacgtt
    10561 agcctgggaa ttaatatcgc acctgtggtt tttactggtg ttagtggtca tgacgacgct
    10621 gtgcgtatgg atatttaaac gcatcagaaa taatttagaa aattctgata aaacgaataa
    10681 aaaattctcg atggtaaaac tatcgctgat ttttttgtgc ctcggcatcg gttatgcggt
    10741 aataagaaga acgattttta ttgtgtatcc gcccattctg agtaatggca tgttcaattt
    10801 tattgtcatg caaacgctat tttatttgcc gttctttatc ctcggcgcac tggctttcat
    10861 tttccctcat cttaaagcct tgtttaccac gccgtctcgt ggctgtaccc tcgctgcagc
    10921 attggcgttt gtcgcttatt tactcaacca gcgctatggc agtggcgatg cctggatgta
    10981 cgaaaccgag tcggtgatca ccatggtcct cggtctgtgg atggtgaatg tggtcttctc
    11041 ctttggccac cgtttgctta acttccagtc agcgcgggtg acttactttg ttaacgcatc
    11101 gctatttatc tatctggttc accacccgtt aacgctgttt ttcggcgcgt acattacacc
    11161 gcacatcacc tccaactggc ttggttttct ctgtggcctg atattcgtag tagggattgc
    11221 gataattctg tatgaaattc atttacgcat cccattactg aagtttttgt tctctggtaa
    11281 accggttgtt aagcgtgaga acgataaagc accagcccgt taagccgcgt ttacaataac
    11341 cattccacgg gcaatatcga cgccagtctg accataaccc gcttccagaa actggtggcg
    11401 ggttcttttt tgagaacaat ctctttcttt gcatgacgat caacccagtt gatccgcccc
    11461 cacctgtcca gacggagctg ccaggccgca tcatactggc tctgaataaa gcgtttatca
    11521 attaactgtg ccagcgtttc gctctctatc acgaagccca tttcagtatt gagcaatgtc
    11581 gaacgtggat cgaaattgaa agagccgata aacaccgttt taccatcgat gctaaaggtt
    11641 ttagcatgca ggctggctcc ggaattaccg gttatgccgc gatcgtgtaa cgtactactt
    11701 tgttcacgcg tcggcttgag ttcatataat tccacgccat agcggagcaa ttttttgcgc
    11761 cagcgcgcgt atccggcatg gacgacagca acatcgttag cggcaagaga attggttagg
    11821 atcgcaatct ttaccccttt tctcaccatc cgtaagagtt gcgccacacc tgcgcgtgtc
    11881 ggtacaaaat aggaagagat aatatcgatg cgttcactgg gtgagcccat gatatcgaac
    11941 aggcgctgcg gtaacagtga atgccgtttt gccttgccct cccctttcgc cggatcatca
    12001 cttaataaac gtgttttcgc ccagataagc ggcaacgttc catcaaccag atgatttata
    12061 aacggactgg attccatttt gcgtaagtaa cgatgcgtca tggcatcgtt atgccaggag
    12121 gcgggtaact cgatgcgatc cgccatttca ccttccggga catccagcac ctgctgtaag
    12181 ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat cggcaacgtc ctctactacg
    12241 ggtcctattg ccatgacatc taaatccgaa aaaagtggct cttcccctgc cccaaaatag
    12301 gcatcaccaa tatttcgccc ccctaccagc gtcaccacgc catcgacagt gaaactttta
    12361 ttgtgcatac ggcgattgag acgggaaaag tcggtgatat aaccaagcgg acgcagcaag
    12421 cgaaacgaga aaggattaaa aagccggact tcaatgcggg gatggctgtc aagcaagcgt
    12481 aaaatgtcgt caagtccggg cgtattgttg tcgtccagca acaaacggac gcgaacgcca
    12541 cgctttgctg cggctaacag ggcggaaaac agtaaccgac ccgacatatc gtcctgccag
    12601 atgtaatact gaacatccag cgtatgctct gccatttcgg ccagacgata ccgggcggca
    12661 aacgcatcca gacttttctc cagagggaaa agaccacact gaccgggatg ttgcgaacac
    12721 agtggcagca ccgcgctcgc cagccggggc aaatcattca tctccttgtt gggtaaggag
    12781 tctttcgtag aggtgggagt tttcttcatc ataacagaca aagtatacct gttcgggtaa
    12841 agcgtgacgg gtaataaatt ctgaaacggt ttttactgcg atttcagccg ctgccgcacg
    12901 agggtaacca taaaccccag tactgattgc aggaaaagcc actgacgtat agctgtttgc
    12961 cgccaccagt cgcaggctat tgagataggc atcctgcaaa agctggtctt cgttttgttc
    13021 accaccacgc cagactggcc cgacggtgtg cactacggct ttagcaggaa gatcgcctgc
    13081 aagcgtaata acggcatgcc ccgtagggca atcgccctgc tgttgcctaa cttttaaaca
    13141 ggcatccagc agggccggac ccgctgcgcg atgaatggcc ccatcgacgc cgccgcctcc
    13201 cattaatgac ggattagccg cattcacaat cacatcaacg gccagtttgg taatatcacc
    13261 ctgcacaaca tgaatacgcg ttttcataac gcctccttac accttcttgt ttcttaagtg
    13321 tacagcaggc tttttggtta aatcgcattt ttctgctcag caacgtgagt tgttcagaaa
    13381 cttagttata ttcgatgttc attacagcca gctttttctg tttatcaaga tactgcactt
    13441 ttactgaggc tacttgcgag tttttaacat tgccgctcat taacgctttg ctggaatagg
    13501 tttttcccgg aacagaacca ttctgtgggc agttcatttc aacgggtgat gactgggtgc
    13561 tgacgtcaca tgccggttct acaatttggc cataaaaatg gatcacgccc ccttgcgcgg
    13621 tgcttcctgc gatggcaata aaaggaaaaa acaaactgcc gagcataaga gagtttaaaa
    13681 aaggtctgaa catttattct atttcctcaa tgattagtca tccttgaggg ttgtgttatc
    13741 catactttca ggttggctga ttcgcagcag accattctct ccagattcat cttatgctcg
    13801 atatttcaac aaattaagac ttttctgaag agggcggcca ttgttgtgat aaatgaagtg
    13861 actgtccatc agaaacagta acaactattt tcacccgatc gtccggggaa atatttaaac
    13921 tcaacttcgt caaagcaatg ggttgattag caggcaatga aagggtcttt tcttgcttcg
    13981 tctgactttg ccctgaactg ccttcgcgca gggacaatat ttgtactctg cacagacaag
    14041 attgagtaag agtgacttca ggaataatgg tatacacatc cccttgctgg gtcgtattaa
    14101 aggttatctg actggaaagt gccgcaagga gtaataacgc attcatattc ttctcccgaa
    14161 aaaaaacagg gcttgcgccc tgtttctgta atacaaatga tgtattagta ctgatgagcg
    14221 gtcgcgttgt taccaaagcc aacctgagtc acgttgacgg aggagttaga tgcagtctgg
    14281 tcaactgcag caccgttgcc accaccgaac tgtttaaccg tcatttcaga atttttgccg
    14341 ttccactgat caagagtagc gctgttaccg aagccacgtt gggtcagatc gattgagctg
    14401 tcatccgagc cctgaccaac atctgcacca ttaccgccgc catgctgggt aatagtcaag
    14461 tcagagttac gggcatcagt ttgcagagca agtgcagagt taccgccacc gtactggtaa
    14521 atgttcagct cagaatttgg gccgctatta ttaccgccac caccgtggtt accgccgccg
    14581 ccgtactgag gaacaacacc tgccagagcg ctaccggaga atacgattgc tgcaattgct
    14641 gctactttta aaagtttcat gtaaaacccc catcggattg atttaaaagt cgaatggaaa
    14701 ttaacgttgt gtcacgcgaa tagccatttg cgactgtctc tgcactacaa ttgccgtttt
    14761 ttgagtacca tactgtgtaa tatttgcttt attaccagaa cctttctgga taatcatcgc
    14821 agtattacca taagcacctt gcgaaatact ggcatcgttg gcactgcccg cctgatcaat
    14881 atatgcaagg ttataatctc ctgtctggtc aatctttgcc cggttgctac taccttcttg
    14941 cgcaacaacc gccaaaagtt ttgagcctcc ctgccgtaac tgagcactat tattagtccc
    15001 agcttgacca attatggctg cctgattaaa tgaagactta ctcaattcat ttaccgcgaa
    15061 gttatattct gaattagcta aatcataacc tgctgcggct gcaatcccag gcgcacccag
    15121 tattgttaac atcataaata acaatttgtt tttcatgttg tcaccctgga cctggtcgta
    15181 catttaagaa attaaatcat ttcaacttgg ttgttaacgc aacctgtatt ttgttaacgc
    15241 tgcgttacga tggaaagtat gtctgcggaa atatttttaa taactcaccc gcgcgttgta
    15301 tttttctttt tttgttcata ccaaagtatt aaaagccacg taaatacgct gttatctacg
    15361 caaaaaatat tttgttttct tttaaatctc cgtttccgct atcaaaaatc accagacagt
    15421 cattcttctt gcccgtcgct gattgctgcg atatgtcttg cgcacaagcc gtgacaaacc
    15481 tcgtcttttt gcagtaaaaa attgtccacg gaggtgtgga gaaaaaacaa caacttttta
    15541 catgacgaaa ggactacacc gaaatatttt ttatatgcat tattagtaag ttatcaccat
    15601 ttgtatgatt ttttaaaatt gt
//
LOCUS       DAZFKF010000110        15358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFKF010000110 DAZFKF010000000
VERSION     DAZFKF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.111"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(80..1231)
                     /gene="argE"
                     /locus_tag="ACHKA1_004239"
     CDS             complement(80..1231)
                     /gene="argE"
                     /locus_tag="ACHKA1_004239"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO5681810.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     NLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHLSNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            1385..2389
                     /gene="argC"
                     /locus_tag="ACHKA1_004240"
     CDS             1385..2389
                     /gene="argC"
                     /locus_tag="ACHKA1_004240"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO5681811.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWSGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAQTQSLI
                     "
     gene            2397..3173
                     /gene="argB"
                     /locus_tag="ACHKA1_004241"
     CDS             2397..3173
                     /gene="argB"
                     /locus_tag="ACHKA1_004241"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO5681812.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            3234..4607
                     /gene="argH"
                     /locus_tag="ACHKA1_004242"
     CDS             3234..4607
                     /gene="argH"
                     /locus_tag="ACHKA1_004242"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO5681813.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLDELQKFCPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FARARLG"
     gene            4874..5791
                     /gene="oxyR"
                     /locus_tag="ACHKA1_004243"
     CDS             4874..5791
                     /gene="oxyR"
                     /locus_tag="ACHKA1_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO5681814.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(5774..7174)
                     /gene="sthA"
                     /locus_tag="ACHKA1_004244"
     CDS             complement(5774..7174)
                     /gene="sthA"
                     /locus_tag="ACHKA1_004244"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO5681815.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            7504..8670
                     /locus_tag="ACHKA1_004245"
     CDS             7504..8670
                     /locus_tag="ACHKA1_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFO5681816.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKLWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            8713..10029
                     /locus_tag="ACHKA1_004246"
     CDS             8713..10029
                     /locus_tag="ACHKA1_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFO5681817.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYATAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIANGILLAIGSTVATYISLFYYGTWA
                     AKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            10133..10783
                     /gene="fabR"
                     /locus_tag="ACHKA1_004247"
     CDS             10133..10783
                     /gene="fabR"
                     /locus_tag="ACHKA1_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO5681818.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            10783..11142
                     /gene="yijD"
                     /locus_tag="ACHKA1_004248"
     CDS             10783..11142
                     /gene="yijD"
                     /locus_tag="ACHKA1_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO5681819.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(11182..12282)
                     /gene="trmA"
                     /locus_tag="ACHKA1_004249"
     CDS             complement(11182..12282)
                     /gene="trmA"
                     /locus_tag="ACHKA1_004249"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO5681820.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMITGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      12396..12586
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12651..14534
                     /gene="btuB"
                     /locus_tag="ACHKA1_004250"
     CDS             12651..14534
                     /gene="btuB"
                     /locus_tag="ACHKA1_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO5681821.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPVNTVLA
                     PTDIVTREDIQRWQSKDLNDVMSHLPGVNISQTGGMGTSSSLYVRGTESRHVLVLIDG
                     VPMARAGIDNAIDISQFPVSLVQRVEYIRGPRSAVYGSGAIGGVVNIITMSNDEKAQI
                     NAGMGTNGYQAYDGVINKRFGDTMVTAAGAYQTTKGFNIQPGSPYSGDSDRDGYRNKL
                     FWGGLQHKFNDNFSGFFRGYSYTANNDYDQGSMYGSATGNEEAQNYNQSWDTGLRYNS
                     GIYSSQLIANYQRLKNYNYTNNLGRYAGDATLDDMEQRYIQWGNSIEVGHGAISGGID
                     WKQEKLTSSSITKSDDYERDTTGLYLTGQQQISNVTLEASGREDHDEQFGWHGTWQTA
                     AGWEFIDGYQATLSYGTSFLSPSLGQQYGAARFASIYGPGIAANPNLKPEESKQWEIG
                     IEGVTGTLDWRLSGYRYEIQNLIDYKSINNVNQYINVKSATIKGLEWTGNITTGPVEH
                     RLTLQYVDPRDDETDKVLYRRAKQQVKYELTGQAYGFEWDVSYQYIGQRYDNAYDETS
                     GSSHTVKLGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGS
                     YTF"
     gene            14479..15336
                     /gene="murI"
                     /locus_tag="ACHKA1_004251"
     CDS             14479..15336
                     /gene="murI"
                     /locus_tag="ACHKA1_004251"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO5681822.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         3557 a         3645 c         4235 g         3921 t
ORIGIN      
        1 atcgcatccg acattattgc ggcactggag tttggcaaca gtgccggatg cggcgcaagc
       61 gccttattcg gcctacgttt taatgccagc aaaaatggtg aatcacctgg gtgatcagtt
      121 cgcgggtggg cttgataaac ctcgtctcca gatattcatc aggctgatgg gcttgattga
      181 ttgaacccgg ccccagaacc agcgtcgggc ataacgtttg aataaacggc gcttcggtac
      241 agtagttcac cacttcggtc tttgctccga gcaatttctc aactacttca accagttgat
      301 gatttggcgg gcattcatag ccagggatcg gcggatgcag ctcgtcgacc gtcagacgac
      361 ccggccagcg ttcgctcacc ggagccaatg catcgttgag caaaccatta agttcattga
      421 gtgtcatgcc aggcagcgga cgaatatcca tatgcaactc acagcaagcg caaatacggt
      481 tagacgcgtc gccaccgtga atatgcccga ggttgagcgt agggtatggc acggtaaacg
      541 cttcgtagtg ataacgttct ttcaggttat cgcgcaattg caaaatatgc ccgatggcgt
      601 cgtgcattag ttcgatagcg ttaactccgc gtgctggatc gctggagtgc cccgactgac
      661 cctgaatacg gatggcgtta gagagatgac ctttatgtgc gcgtaccggt tgtagtgacg
      721 tcggttcgcc aatgatggcg caatccgggc gcagggcggt agtttcggca aaataacgcg
      781 ctccggccat actggtttct tcatcagcag tcgccagaat gtagagcggt tttttcagtt
      841 tcgtgacgtc gacatcgcgt agcgcatcaa ggataaacgc aaaaaagcct ttcatgtcgg
      901 cggtgcctaa gccgtaaagc ttgccgtcat gctccgtcag tgtaaacgga tcgcgcgtcc
      961 agcgaccgtc atcaaatggc accgtatcgg tatgccccgc cagcaacaag ccgccagccc
     1021 cctgtccggt acttgccagc atattgaatt tgttgcgagt tcctggcaca ggctgcactt
     1081 ccacattgaa gcctaaattt ttaaaccagt ccgccagcag agtgattaaa tctgcattgc
     1141 tttgatcgag tgcctcttcc gtggcgctta ttgaaggtgt ggcaatcaga gcacggtaaa
     1201 tctcgataaa tggcggtaat ttgtttttca ttgttgacac acctctggtc atgatagtat
     1261 caatattcat gcagtattta tgaataaaaa tacactaacg ttgagcgtaa taaaacccac
     1321 cagccgtaag gtgaatgttt tacgtttaac ctggcaacca gacataagaa ggtgaatagc
     1381 cccgatgttg aatacgctga ttgtgggtgc cagcggctac gctggcgcag agctagtgac
     1441 ctatgtaaat cgccatccgc atatgaacat aaccgctttg actgtttcag cgcaaagcaa
     1501 tgatgcggga aagttaatct ccgatttgca tccgcagcta aaaggcatcg ttgatctgcc
     1561 gttgcagccg atgtcggata tcagcgagtt tagcccaggg gtggacgtag tgtttctcgc
     1621 caccgcccat gaagttagcc acgatttagc gccgcagttt cttgaagcgg gctgcgtggt
     1681 gttcgacctt tccggcgcgt ttcgtgttaa cgacgccacc ttctatgaaa aatattacgg
     1741 ctttacccat caatacccgg aactgctgga acaggcagcc tacggtctgg cggagtggag
     1801 cggtaataaa ttaaaagaag cgaatttgat tgcggtgcca ggctgttatc cgacggcggc
     1861 acagctggcg ctgaaaccgt tgattgatgc cgatcttctt gacctcaatc agtggccggt
     1921 gatcaacgcc accagcggcg tgagcggtgc aggacgtaag gcagcgattt caaatagttt
     1981 ttgtgaagtt agcctgcaac cgtatggcgt ctttactcat cgccatcaac cagagatcgc
     2041 cacacacctc ggtgctgacg ttatcttcac cccgcatctg ggcaatttcc cacgcggcat
     2101 tctcgaaacc attacctgcc gcctgaaacc gggtgtgacc caggcgcaag tcgcgcaagt
     2161 gttacagcag gcgtatgccc ataaaccgct ggtgcggctg tatgacaaag gcgttccggc
     2221 gctgaaaaat gtcgttgggc tgccattttg cgatatcggg tttgccgttc agggcgagca
     2281 tttgattatt gtggcgaccg aagacaactt actgaaaggc gcggcggcac aagcggtaca
     2341 gtgcgccaat attcgtttcg gctatgccca aacgcagtct cttatttaag ggtgcaatga
     2401 tgaatccatt aattatcaaa ctgggtggcg tactgctgga tagtgaagag gcgctggagc
     2461 gtctgtttag cgcactggtg aattatcgtg agtcacatca gcgtccgctg gtgattgtcc
     2521 acggcggcgg ttgcgtggtg gatgagctga tgaaagggct gaatctgccg gtgaaaaaga
     2581 aaaatggcct gcgggtgacg cctgcggatc agatagacat tatcactgga gcactggcgg
     2641 gaacggcaaa taaaaccctg ctggcatggg cgaagaaaca tcagattgcg gccgtaggtt
     2701 tgtttctcgg tgacggcgac agcgtcaaag tgacccagct tgatgaagag ttaggccatg
     2761 ttggactggc gcagccaggt tcgcctaagc ttatcaacac cttgctggag aacggttatc
     2821 tgccggtggt cagctccatt ggcgtaacag acgaagggca actgatgaac gtcaatgccg
     2881 accaggcggc aacggcgctg gcggcaacgc tgggcgcgga tctgattttg ctctccgacg
     2941 tcagtggcat tctcgacggc aaagggcaac gcattgccga aatgaccgcc gcgaaagcag
     3001 aacaactgat tgagcagggc attattactg acggcatgat agtgaaagtg aacgcggcgc
     3061 tggatgctgc gcgcacgttg ggccgtccgg tagatatcgc ctcctggcgt catgcggagc
     3121 agcttccggc actgtttaac ggtatgccga tgggtacgcg gattttagct taagttttgt
     3181 tggccggagg cgcagttttc cggcattgaa tttcaaaata aggaaacaga gttatggcac
     3241 tttggggcgg gcgttttacc caggcagcag atcaacggtt caaacaattc aacgactcac
     3301 tgcgctttga ttaccgtctg gcggagcagg atattgttgg ctctgtggcc tggtccaaag
     3361 ccctggtcac ggtaggcgtg ttaaccgcag aagagcaggc tcaactggaa gaggcgctga
     3421 acgtgctgct ggaagatgtt cgcgccaggc cacaacaaat ccttgaaagc gacgccgaag
     3481 atatccatag ctgggtggaa ggcaaactga tcgacaaagt gggccagtta ggcaaaaagc
     3541 tgcataccgg gcgtagtcgt aatgatcagg tagcgactga cctgaaactg tggtgcaaag
     3601 ataccgttag tgagttactg acggctaacc ggcagctgca atcggcgctg gtggaaaccg
     3661 cacaaaacaa tcaggacgcg gtcatgccag gttacactca cctgcaacgc gcccagccgg
     3721 tgacgttcgc gcactggtgc ctggcctatg ttgagatgct ggcgcgtgat gaaagccgtt
     3781 tgcaggatgc gcttaagcgt ctggatgtca gtccgttagg ctgtggcgcg ctggcgggaa
     3841 cggcttatga gatcgaccgt gaacagttag ctggctggct gggctttgct tcagcgaccc
     3901 gtaacagtct cgacagcgtt tctgaccgtg accacgtgct ggaactgctg tcggctgccg
     3961 ctatcggcat ggtgcatctg tcgcgttttg ctgaagatct gattttcttt aacaccggcg
     4021 aagcggggtt tgtggagctt tctgaccgcg tgacttccgg ttcatcatta atgccgcaga
     4081 agaaaaaccc ggatgcgctg gagctgattc gcggtaaatg cggtcgggtg cagggcgcgt
     4141 taaccggcat gatgatgacg ctgaaaggtt tgccgctggc ttacaacaaa gatatgcagg
     4201 aagacaaaga aggtctgttc gacgcgctcg atacctggct ggactgcctg catatggcgg
     4261 cgctggtgct ggacggcatc caggtgaaac gtccacgttg ccaggaagcg gctcagcagg
     4321 gttacgccaa cgccaccgaa ctggcggatt acctggtggc gaaaggcgta cctttccgcg
     4381 aggcgcacca tattgttggt gaagcggtgg tggaagccat tcgtcagggc aaaccgctgg
     4441 aagaactgcc gctcgacgag ttgcagaaat tctgcccggt gattggcgaa gatgtctatc
     4501 cgattttgtc gctgcaatcg tgcctcgaca agcgtgcggc aaaaggcggc gtctcaccgc
     4561 agcaggtggc gcaggcgatt gcttttgcaa gggcgcggtt ggggtaagaa catttatatg
     4621 tacaaatttg agcctggctt atcgccgggc ttttttatgg caaaaaaaag cggatcctgg
     4681 agatccgcaa aagttcacgt tggctttagt tattcgagtt gagaaactct cgaaacgggc
     4741 agtgacttca agggttaaaa gaggtgccgc tccgtttctg tgagcaatta tcagtcagaa
     4801 tgcttgatag ggataatcgt tcattgctat tctacctatc gccatgaact atcgtggcga
     4861 tggaggatgg ataatgaata ttcgtgatct tgagtacctg gtggcattgg ctgaacaccg
     4921 ccattttcgg cgtgcggcag attcctgcca cgttagccag ccgacgctta gcgggcaaat
     4981 tcgtaagctg gaagatgagc tgggcgtgat gttgctggag cggaccagcc gtaaagtgtt
     5041 gttcacccag gcgggaatgc tgctggtgga tcaggcgcgt accgtgctgc gtgaggtgaa
     5101 agtccttaaa gagatggcaa gccagcaggg cgagacgatg tccggaccgc tgcatattgg
     5161 tttgattccc acggttggac cgtacctgct accgcatatt atcccgatgc tgcaccagac
     5221 ctttccaaag ctggaaatgt atctgcatga agcacagacc caccagttac tggcgcaact
     5281 ggacagcggc aaactcgatt gcgtgatcct cgcgctggtg aaagagagcg aagcattcat
     5341 tgaagtgccg ttgtttgatg agccaatgtt gctggctatc tatgaagacc acccgtgggc
     5401 gaaccgcgaa tgcgtaccga tggccgatct ggctggggaa aaactgctga tgctggaaga
     5461 tggtcactgt ttgcgcgatc aggcaatggg tttctgtttt gaagccgggg cggatgaaga
     5521 tacacacttc cgcgcgacca gcctggaaac tctgcgcaac atggtggcgg caggtagcgg
     5581 gatcacttta ctgcctgcgc tggctgtgcc gccggagcgc aaacgcgatg gggttgttta
     5641 tctgccgtgt attaagccgg aacctcgccg cactattggc ctggtttatc gtcctggctc
     5701 accgctgcgc agccgctatg agcagctggc agaggccatc cgcgcaagaa tggatggcca
     5761 tttcgataaa gtgttaaaac aggcggttta agccatttag cgcagctacc cgataggctt
     5821 ccgccatcgt cgggtagtta aaggtggtgt tgacgaagta ctcaatagtg ttgccgccac
     5881 ctttctgttc cataatcgcc tggccgatat gaataatttc ggcagcgcgc tcgccaaagc
     5941 agtgaatgcc cagaatctct ttcgtttccc gatggaacaa aattttcagc gtgcccacgt
     6001 tcatgccgac gatttgtgcg cgtgccagat gtttgaactg ggcgcggccc acttcatacg
     6061 gtactttcat cgcggtcagc tgctgttcgg ttttgcccac agagctgatt tccgggatgg
     6121 tgtaaatacc ggtagggata tcttcaatca gatgtgcggt ggcttcacct ttcaccagcg
     6181 cctgcgcggc aatgcgcccc tggtcatagg ccgccgacgc caggctcgga taaccaatca
     6241 cgtcgcccac cgcgtaaacg tgcggctgtg cggtctgata catactgttg accttcagct
     6301 gtccgcgact gtcagtttcc agcccaatgt tctgtaacgc cagcgaatca gtattaccgg
     6361 tgcgaccgtt ggcatagagc aggcagtcag ctttcagttt tttacccgac ttcagatgca
     6421 tgatcacacc gtcgtcacag ccttcgatct tctcgtactc ttcgttgtgg cgaatcacta
     6481 cgccactgtt ccagaagtga taggagagag aatctgacat ctcttgatcg agaaatgcca
     6541 gcaggcgatc gcgggtgttg atcagatcca cttttacatc cataccgcgg aagatcgacg
     6601 catattcaca gccgatcact ccagcaccat agataagtac atggcgcggt tcgtggtgca
     6661 tgctaagaat tgagtcgctg tcgtaaatgc gtggatgggt gaaatcaaca tctgttggat
     6721 gatatggacg agagccgcag gcaataacaa atttttcagc ggttagtgtt tcaacgctgc
     6781 cgtccgggca atccagcgcc aacgtatgct cgtcaacaaa gcgagcgttc ccctgcaata
     6841 tttcacagtg attacgttcg taaaatccct gacgcatgcg cgtttgttga ttaatcacgt
     6901 tatcggcatg gttaaggata tcggcaaaag aagagcggag cagtcgggaa tggtcgctgt
     6961 atagtgggtt ttgattgaat tctataatgc ggctgacggc gtgacggaga gctttcgacg
     7021 ggatggtgcc ccagtgggtg caaccgccgc caacattttg ataacgctcg ataactgcga
     7081 cgcgcgcacc ttgcttaacc aggcccattg cagcgccttc gccgccgggg ccggaaccta
     7141 ttactatggc atcgtaatcg taggaatgtg gcatggtagg gcttacctgt tcttatacat
     7201 aaaagcaaca gaatggtaac attttatcgc gggtaagcca attgatcccc gtcattaatc
     7261 tggctatagc gtgagcggcc tttgctttgt ctgcctcctt cttaacccat ttattcacta
     7321 ttaatcgctt tttattatgg ctgatagttt tatcttgccg aagcgtgctt ttttgttatt
     7381 gtctttatga aatcttctta taaaacaaac atataaatat tttatattga tgggttattt
     7441 gtatttatac catcaataaa atccgttaat tagcctgttt ataagtgaat aaaggaatag
     7501 ccgatgatcg aacactatat tcgagggttt gaggaagaac tgcgtgagat ccgccatcag
     7561 atccacgaaa acccggagct gggattacaa gaatttaaaa ccagtgcttt ggttgcggaa
     7621 aagctgcgtc agtggggcta tgaagtagag caaggactgg cgacgacggg gatagtggcc
     7681 acgttaaaag tgggcgacgg agagaaaagt attggcctgc gggcggatat ggatgcgctg
     7741 ccaatttatg aaaatagtgg caagctctgg gccagtaaac atcctggttt aatgcacgcc
     7801 tgcgggcatg acgggcatac caccatactg ctgggggccg cgcgttattt tgccgaaacc
     7861 cgccgtttta acggcacgtt acgtcttatc ttccagcctg cggaagagat gattaatggc
     7921 ggtgagatca tggttaaaga ggggcttttt gatcatttcc cctgcgatgt cattttcggc
     7981 atgcataaca tgcccggtct gccggtgggt aagttttatt tccagccagg ggcactgatg
     8041 gcgtcaatgg atcagttcca tattacggtt cgtggttgtg gcggacacgg tgcgatcccg
     8101 cacaaagcca tcgatccggt gctggttgcc gcacatatca ccaccgcatt acaaagcatt
     8161 gtgtcacgca atgtcgatcc gctggaagcg gcggtaatta ccgtcggcag cattgttgcg
     8221 ggtgaggcgg ctaacgtcat tcctgacagt gctgagatga aaatcagcgt ccgctctctt
     8281 agccgcgata cccgacaact tcttctgacc cgtattcctg ctctggcaca agcccaggct
     8341 gccagctttg gcgctacggc tgaggttacg catgttaacg gtacaccagt attagtaaat
     8401 gacgaggaga tggcgcgctt cgcctggcag gtggcgtgta aaacgtttgg tgaagatcgg
     8461 gccgagtttg gcatcaagcc tctgatgggc agtgaagatt tctctttcat gctggaagcc
     8521 caacccaaag gcggtttcct gttattcggt aatggcgatg tcggggaagg ttcctgcatg
     8581 gtgcataacc cgggttacga cttcaacgat gcaagtctgg tcccggcaag cagctactgg
     8641 ggcgcactgg tagaagcctg gctgcaataa ataaaacaac acacaacaac actttttggc
     8701 aaggaacatg gaatgacggc gatcgtaaat gaaaccgtga gtgtgaagaa aaagcccagc
     8761 ggcaggcggg tgatttttgc ctcggcgttt ggcaatgcgc tcgaattttt tgattttggc
     8821 gtttacaact tttttgtggt ctatatcagt acacttttct tcccacccag cgctgatcac
     8881 aatgtcgcgc tgttactggc atttgcaacg tttggcgtga gtttttttat gcgcccgctt
     8941 ggtggaatta tcgtcggggc ctgggccgat cgtttcggac gcaaacccgc gatggtgttc
     9001 actattgccc tgatgagcct cggcacgttg atgattggca ttgcgccgac gtatgcgacg
     9061 gcgggctatt ggggaacggc aactctggta cttgctcgtt tgattcaggg tgttgcagca
     9121 ggcggtgaag ttggggcttc aatgtcgcta ctggtggagt cagctccggc aaatcgtcgc
     9181 ggtttttaca gcagttggtc actggcgact caaggcctgg caacaacttt tggaggtgtt
     9241 gtcgctttag gtttaagtgc atggctgccc tttgcgaccg gttcagaaac cgtcatggct
     9301 gaatggggct ggcgtgtgcc gttttttatt ggcgtgttat tagcgcctgt cggctgttgg
     9361 ttgcgtttga gtctggaaaa tgacgtacct gagcctgtgc gcaataaaaa agctgctacc
     9421 agcgaaagcg cgttttctct gctcctgcaa cataaggcga ctatcgctaa cggtatcttg
     9481 ctcgctattg gtagtacagt cgcaacctat atctccctct tttattacgg tacatgggcg
     9541 gcgaagtatt taggaatgaa ccaaaactat tctcatgcag cgatgttact ggcgggcgtt
     9601 attacgtttg tgggtgcgct gctggtgggg atgttgtgcg attcggtcgg gcgtaaaaag
     9661 ctgattttaa tctcccgtgt gatggtgctg atctgtagct ggccgtcatt ctggctgttg
     9721 gtgaattacc caagccctgg catgttgctg acggtggttt tcgtgatggt cagctttacc
     9781 acgcttggcg gtgtgccagt gatgttgttg atctctgaac tgttgccgaa acggattcgg
     9841 gcattgggtt ttgcgctggt ttatagcatt ggtgtggcaa tctttggcgg ttttgctcaa
     9901 tatttcgcta cgcaatccat cgtgttgctg gatagtttga cggctccggc gtggtatttg
     9961 ggtggaggca cgttgctgtc gatgttggcg ttgttgtacg taaaagaacc ggccaaagaa
    10021 ttgcagtaaa tatgttttat tgcgttaccg ttcattcaca atactggagc aatccagtat
    10081 gttcattctc tggtatagtg ccagcagtac ttttggcaag gattcagaca tcgtgatggg
    10141 cgtaagagcg caacaaaaag aaaaaacccg ccgttcgctg gtggaagccg catttagcca
    10201 attaagtgct gaacgcagct tcgccagcct gagtttgcgt gaagtggcgc gtgaagcggg
    10261 cattgctccc acctcttttt atcggcattt ccgcgacgta gacgaactgg gtctgaccat
    10321 ggttgatgag agcggtttaa tgctacgcca actcatgcgc caggcgcgtc agcgtatcgc
    10381 caaaggcggg agtgtgatcc gtacctcggt ctccacattt atggagttca tcggtaataa
    10441 tcctaacgcc ttccggttat tattgcggga acgctccggc acctccgctg cgtttcgtgc
    10501 cgccgttgcg cgtgaaattc agcacttcat tgcggaactt gcggactatc tggaactcga
    10561 aaaccatatg ccgcgtgcgt ttactgaagc gcaagccgaa gcaatggtga caattgtctt
    10621 cagtgcgggt gccgaggcgt tggacgtcgg cgtcgaacaa cgtcggcaat tagaagagcg
    10681 actggtactg caactgcgaa tgatttcgaa aggggcttat tactggtatc gccgtgaaca
    10741 agagaaaacc gcaattattc cgggaaatgt gaaggacgag taatgaaaca agcaaatcaa
    10801 gatagaggta cgctgctgct ggcgttggtt gctggcttat cgattaatgg tactttcgca
    10861 gcgctgttta gctccattgt gccattttct gtattcccga ttatttccct ggtgctgacg
    10921 gtttactgcc tgcatcaacg ttatcttaat cgcaccatgc cggtaggctt gccgggtctg
    10981 gcagctgcct gttttattct cggcgtactg ctgtacagca cggtagttcg tgcggaatat
    11041 ccggatatcg gctctaactt cttcccggca gtactctccg tcattatggt gttctggatt
    11101 ggcgcgaaga tgcgtaaccg taagcaggaa gttgctgagt aatcggtatc atgccggatg
    11161 ctggcgcatc cggcatgggt tttacttcgc ggtcagtaat acgccgcact ccatatggtg
    11221 cgtgtagggg aactgatcaa acagagccag acgttcgacc ttgtgcgtct ggcttaatgt
    11281 ttccagattc ttgcataacg tttccggatt gcaggagata tacagaatac gtggatacgc
    11341 ctgcaccatt ttctcggttt cactgtccag accgctgcgc ggtgggtcga caaaaatggt
    11401 ttcgcactga taactcttta agtcgatccc ttgcaggcgg ttaaactcgc gcacgccatt
    11461 catcgcctga gtaaattctt ctgccgccat acgaataatt tgtacgttat caatatggtt
    11521 agcggcaata ttgtattgcg cggcagcgac tgacggcttg gcgatttcgg tggctaatac
    11581 ccgatcaaaa ttgcgcgcca gggctaatga gaagttaccg ttgccgcagt acagctccag
    11641 taaatcgcct tttgagcctt tggttacgtc cagcgcccat tccagcatct gaatattcat
    11701 cgccgcgttc ggctgggtaa agctgttttc tacctgacgg tagatcatct ctttccctgc
    11761 gaccggcaga cgttcgtcga tgtaatcttg atccagcgcg attttggttt tcgttgcccg
    11821 accaatcaga tgcacattca gattctgtgc tcgcagtgca tcgcgcaggg cttccgcttc
    11881 ctgacgccac tcatcatcca gctttttatg gtatagcagg gaaacgaccg cctgattgct
    11941 cagcgtggtg aggtaatcaa tctggaacaa cttgtggcgc agaacgggat tattacgcac
    12001 acccgtaatc atcgccgtca tcaactggtt gatgagttca ctggcggcgg ggaagctatc
    12061 cacgcggata cggcttttgg tttgttgatc gaaaatgatg tgatacaggt catcgccatc
    12121 atgccagatg cggaactccg cacgcatccg gtaatgactg accggcgagc gaaacacttc
    12181 cggaaccagg tcagaaaacg gtgccatcat actttgcaaa cgtaccactt tttcggctaa
    12241 ctgcgcttca tactgttctg ttggaaggtg ttcgggggtc atgatgtatc ctgaaaaatt
    12301 aaagtacgcg gcgattgtag ggattgctca tcagatgtcc agatcttgat gaattcctat
    12361 ttgtgagcta cgtctggaca gtaacttgtt acaacctgta gcatccactt gccggtcctg
    12421 tgagttaata gggaatccag tgcgaatctg gagctgacgc gcagcggtaa ggaaaggtgc
    12481 gatgattgcg ttatgcggac actgccattc ggtgggaagt catcatctct tagtatctta
    12541 gatacccctc caagcccgaa gacctgccgg ctaacgtcgc atctggttct catcatcgcg
    12601 taatattgat gaaacctgcg gcatccttct tctattgtgg atgctttaca atgattaaaa
    12661 aagcttcgct gctgacggcg tgttctgtca cagccttttc cgcttgggca caggatacca
    12721 gcccggatac tctcgtcgtt actgctaacc gttttcagca gccagtaaat actgtccttg
    12781 cacctactga tattgttacc cgtgaggata ttcaacgctg gcagtcgaaa gacctcaacg
    12841 atgttatgag tcatctcccc ggtgtaaata tctctcaaac tggtggaatg gggacgagtt
    12901 cttctcttta tgttcgtggc actgaatcgc gccatgtttt agtgttgatt gacggtgtgc
    12961 cgatggctcg tgccggtatc gacaatgcaa ttgatattag ccagttcccg gtatcacttg
    13021 tacagcgtgt tgaatatatt cgtgggccgc gttctgccgt ttatggttct ggtgccatcg
    13081 gaggtgtggt gaacattatt acaatgagta atgatgagaa agctcagatc aacgcaggaa
    13141 tgggtacaaa tggttatcaa gcctatgatg gggtaataaa taaacgtttt ggcgatacga
    13201 tggtaacagc agcaggtgct taccagacga ctaaaggttt taacattcag cctggctcac
    13261 catacagtgg tgatagcgat cgcgatggat atcgcaacaa acttttctgg ggggggctgc
    13321 agcataagtt taacgataac ttctcggggt tcttccgtgg ttacagctac acagctaata
    13381 atgattacga ccagggctcg atgtatggtt ctgctactgg caacgaagaa gctcagaatt
    13441 acaaccagtc ctgggatact ggcctacgct ataactccgg catctattcg tctcagttaa
    13501 ttgctaacta tcaacgactc aagaattata actacacaaa taatcttggt cgttatgcgg
    13561 gagatgccac cctggatgac atggaacagc gttatattca gtggggtaat agcattgagg
    13621 tagggcatgg tgcaattagt ggcgggatcg attggaaaca ggaaaaactg acgtccagca
    13681 gcattacaaa atcagatgat tacgaacgcg ataccaccgg gttatacctg actggacagc
    13741 aacagatttc caatgtgact ttggaagctt ctggccgaga agatcatgat gaacagtttg
    13801 gctggcatgg tacctggcag acagctgctg gctgggagtt tattgatggc taccaggcaa
    13861 cactttctta cggtaccagt tttctttcgc cttctcttgg acagcaatac ggggctgctc
    13921 gctttgcttc aatttatggt cctggtattg cagccaaccc aaatttgaag ccagaagaat
    13981 ctaaacagtg ggagattgga attgaggggg taactgggac tctggattgg cggctctcag
    14041 gatatcgtta tgaaattcag aacctgattg attataaaag cataaataat gtcaaccagt
    14101 acattaatgt gaaatcagcc acgattaaag ggctggaatg gacgggaaat attacaaccg
    14161 gtcctgttga acatcgcttg acgttacaat atgttgaccc gcgcgatgat gaaacagata
    14221 aagtgctgta ccgtcgggca aaacagcagg ttaagtacga gttgactgga caagcttatg
    14281 gtttcgaatg ggacgtttct taccaatata taggtcaacg atacgacaac gcttatgacg
    14341 agacctctgg ctcatcacat acagttaaat taggcggtgt gagcttgtgg gatcttgcgg
    14401 ttgcatatcc ggtcacctct cacctgacag ttcgtggtaa aatagccaac ctgttcgaca
    14461 aagattatga gacagtctat ggctaccaaa ctgcaggacg ggaatacacc ttgtctggca
    14521 gctacacctt ctgaaccacg tcccaccgtg ctggtgtttg actccggcgt cggtgggttg
    14581 tcggtctatg acgagatccg gcatctctta ccggatctcc attacattta tgctttcgat
    14641 aacgtcgctt tcccgtatgg cgaaaaaagc gaagcgttta ttgttgagcg agtggtggca
    14701 attgtcaccg cggtgcaaga acgttatccc cttgcgctgg ctgtggtcgc ttgcaacacc
    14761 gccagtaccg tttcacttcc tgccttacgc gaaaagttcg acttcccggt tgttggtgtc
    14821 gttccggcga ttaaacctgc tgcacgtctg acggcaaatg gcattgtcgg attactggca
    14881 acccgcggaa cagttaaacg ttcttatact catgagctga tcgcgcgttt cgctaatgaa
    14941 tgccagatag aaatgctggg ctcggcagag atggttgagt tggctgaagc gaagctgcat
    15001 ggcgaagatg tttctctgga tgcactaaaa cgtatcctac gcccgtggtt aagaatgaaa
    15061 gagccgccag ataccgttgt attgggttgc acccatttcc ctctactaca agaagaactg
    15121 ttacaagtgc tgccagaggg aacccggctg gtggattctg gcgcagcgat tgctcgccga
    15181 acggcttggt tgttagaaca tgaagccccg gatgcaaaat ctgccgatgc gaatattgcc
    15241 ttttgtatgg caatgacgcc agaagctgaa caattattgc ccgttttaca gcgttacggc
    15301 ttcgaaacgc tcgaaaaact ggcagtttta ggctgatttg gttgaatgtt gcgcggtc
//
LOCUS       DAZFKF010000111        15113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFKF010000111 DAZFKF010000000
VERSION     DAZFKF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.113"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..278
                     /locus_tag="ACHKA1_004252"
     CDS             <1..278
                     /locus_tag="ACHKA1_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593043.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFO5681823.1"
                     /translation="SPAINDKKSDAGVNVPAGGIEQELKMKPEEEMEQQLPPGISESG
                     EVVDMAVYEAWQREQNPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            278..5548
                     /gene="traI"
                     /locus_tag="ACHKA1_004253"
     CDS             278..5548
                     /gene="traI"
                     /locus_tag="ACHKA1_004253"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO5681824.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQ
                     GSVDKDVFTRLLEGKLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPEAGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNIYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GQREVTMTVLSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKIPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVSDSPFTALKLESGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLESAISHQ
                     KSALHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVKMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEVREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLSTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGSGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGLEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAERLFSTARELRDV
                     AVGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAEEKPDQPDGKTEQAVRAIAGQEQDRASSSERETALPESVLRESQRVQDAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5568..6314
                     /gene="traX"
                     /locus_tag="ACHKA1_004254"
     CDS             5568..6314
                     /gene="traX"
                     /locus_tag="ACHKA1_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HFO5681825.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDTIKTVALVLM
                     VLDHANRILHLEQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQASVNRLWGWAVVAQF
                     GYFLAGYPWYEGNILFAFAVAAQMLKWCEYHSLVWSVSALTLLVLWFPLSSTSYGAVG
                     IILLMLCHRIYRTTHLSVRSMLALCLFPVVALVNLQDSVTAAVSGVLVTYMVVRMVSG
                     IRWPAARFWPADFFPVFYVCHLAVLGVMAL"
     gene            6394..6765
                     /locus_tag="ACHKA1_004255"
     CDS             6394..6765
                     /locus_tag="ACHKA1_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HFO5681826.1"
                     /translation="MMKKILLVAAITGMFVQGVWAKSDQQIRDEAKNNVITVSQVSDM
                     TEGTGVTLNGQIVKQLAAEEDHFELSDASGSIVIDVDDEVWRPLALKAGDKVQVVGEV
                     DISDEGNRKATVDVLTLERLR"
     gene            6801..7358
                     /locus_tag="ACHKA1_004256"
     CDS             6801..7358
                     /locus_tag="ACHKA1_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO5681827.1"
                     /translation="MTEQKRPVLTLKRRTDVETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVSQRNIPLSHKKLSRALKAITRSESYLCAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            7511..7714
                     /locus_tag="ACHKA1_004257"
     CDS             7511..7714
                     /locus_tag="ACHKA1_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681828.1"
                     /translation="MAVSSGLSEHSGIYRYQRVGRHAVRQEIRGFFREAAEAWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            8457..8918
                     /locus_tag="ACHKA1_004258"
     CDS             8457..8918
                     /locus_tag="ACHKA1_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO5681829.1"
                     /translation="MKKYIPLVLFVFSCQVFSADIHGRVVRVLDGDTIDVMLSQHPVR
                     VRLVNIDAPEKKQDYGRWSEKIMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQGEARQQKRGLWADSAPVPPWVWRSRK"
     gene            9021..9404
                     /locus_tag="ACHKA1_004259"
     CDS             9021..9404
                     /locus_tag="ACHKA1_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681830.1"
                     /translation="MNYQAVSELITSSNHNVLIVGDSASEVDSFLNKLNITDYKYYDF
                     SQIYSCSDRTLNDYAVIFIRDALNASEHIIIFNCTGWPDLNNESAVMQFARVARKSGK
                     QLIVAVREQDMKKMEAEFGRIIKIH"
     gene            9684..9833
                     /locus_tag="ACHKA1_004260"
     CDS             9684..9833
                     /locus_tag="ACHKA1_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5681831.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            10117..10377
                     /locus_tag="ACHKA1_004261"
     CDS             10117..10377
                     /locus_tag="ACHKA1_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO5681832.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            10602..10676
                     /gene="tap"
                     /locus_tag="ACHKA1_004262"
     CDS             10602..10676
                     /gene="tap"
                     /locus_tag="ACHKA1_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO5681833.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            10669..11526
                     /gene="repA"
                     /locus_tag="ACHKA1_004263"
     CDS             10669..11526
                     /gene="repA"
                     /locus_tag="ACHKA1_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO5681834.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVVWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
     gene            12440..12709
                     /gene="yacA"
                     /locus_tag="ACHKA1_004264"
     CDS             12440..12709
                     /gene="yacA"
                     /locus_tag="ACHKA1_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFO5681835.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            12709..12987
                     /gene="yacB"
                     /locus_tag="ACHKA1_004265"
     CDS             12709..12987
                     /gene="yacB"
                     /locus_tag="ACHKA1_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFO5681836.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAG
                     QPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            13033..13881
                     /locus_tag="ACHKA1_004266"
     CDS             13033..13881
                     /locus_tag="ACHKA1_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO5681837.1"
                     /translation="MNNDELATRRAQAIAENRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKRPLTSKQLLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDASGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGDRPVIIFNSRFDIRILKQTAAAHSDPADWLEEMTVYCAMEL
                     AAGYYGATNRYGTISLACAASQAGLTWEGQAHSAIADARMAAGVVNAIAAYHLELLQE
                     QARLKI"
     gene            14022..14480
                     /locus_tag="ACHKA1_004267"
     CDS             14022..14480
                     /locus_tag="ACHKA1_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681838.1"
                     /translation="MRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQRAARLAASV
                     KRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVFGEKGRQHR
                     SADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKKEVG"
     gene            14782..15113
                     /locus_tag="ACHKA1_004268"
                     /pseudo
     CDS             14782..15113
                     /locus_tag="ACHKA1_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
BASE COUNT         3707 a         3636 c         4481 g         3289 t
ORIGIN      
        1 tgtctcctgc catcaacgat aagaagtcag atgcaggtgt gaatgttccg gcagggggga
       61 tcgagcagga gctgaaaatg aaaccggaag aagagatgga acagcaactg ccacccggga
      121 tcagtgaatc cggtgaagtg gtggatatgg ccgtttatga ggcatggcaa cgggagcaga
      181 atccggatat ccagcagcag atgcagcgcc gtgaagaggt gaatatcaac gtgcaccggg
      241 agcgcgggga ggatgttgag ccgggagatg atttctgatg ttgagtttca gcgttgttaa
      301 atcggcaggc agtgccggaa actattacac cgacaaggat aattactatg tgctgggtag
      361 catgggggaa cgctgggcgg gcaagggggc tgaacagctt gggctgcagg gcagtgttga
      421 taaggatgtc tttacccgtc ttctggaggg gaagctgccg gacggggcgg atttaagccg
      481 tatgcaggat ggcagtaata agcatcgtcc cggctatgac ctgaccttct ccgcccccaa
      541 aagtgtctcc atgatggcca tgttaggtgg cgataagcgc ctgattgatg cacataatca
      601 ggccgtggat tttgctgtcc gtcaggtgga ggcgctggct tccacacggg tgatgacgga
      661 cggacagtca gaaacggtac tgaccggtaa tctggtgatg gcactcttta accacgacac
      721 cagtcgcgat caggacccac agttacacac gcatgcggtg gtggctaatg tcacgcagca
      781 taacggcgaa tggaagacac tgagcagtga taaagtgggg aaaaccggtt tttccgagaa
      841 cgtgctcgcg aaccgtattg cgttcgggaa aatatatcag agtgagttac gtcagcgcgt
      901 tgaggcgctg ggctatgaaa ctgaagtggt gggtaagcac ggtatgtggg agatgccggg
      961 cgtaccggtg gaggcctttt ccggacgctc acaggctatc cgggaggccg tcggggaaga
     1021 cgcctcgctg aaatcccggg atgtggcggc actggatacg cgaaaatcca aacagcacgt
     1081 cgatcctgaa gtcagaatgg ctgaatggat gcagacgctg aaggaaaccg ggttcgacat
     1141 ccgggcgtat cgtgacgcgg cagaccaacg ggcggagacc cgcacgcaga cacccggacc
     1201 cgcttcacag gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag
     1261 tgaacgaaaa gtgcagttca cgtacacgga cgtgctggcc aggacggtcg gcatactgcc
     1321 gccggaagcc ggtgtgattg agcgggcgcg tgccggtatc gatgaggcca tcagccgtga
     1381 gcagcttatc cccctcgacc gtgagaaggg gctgttcacg tccgggattc atgtgctcga
     1441 tgagctgtcc gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgactgtaca
     1501 tccggagaaa agtgttcccc ggacggccgg ttacagcgat gcggtcagtg ttctggcaca
     1561 ggatcgcccg tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg
     1621 ggtggctgaa ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttattgccgc
     1681 tgaccgtcgc tcacagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac
     1741 cggacgtcgt cagctgctgg aaggcatggc cttcacgccg ggcagtaccg ttatcgtcga
     1801 ccagggcgaa aaactctccc tgaaagagac gttaaccctg ctggacggtg ccgcacgtca
     1861 taacgtacag gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcgctgat
     1921 ggccatgaag gatgccgggg tgaacatata tcgctggcag gggggagagc agcgaccggc
     1981 aaccatcatc agtgaaccgg accgtaatgt ccgctatgcc cggctggccg gagattttgc
     2041 ggccagcgtg aaagccggag aagagagcgt ggcacaggtc agcggagtac gggaacaggc
     2101 catactgaca caggccattc gcagtgaact gaaaacgcag ggcgtactcg ggcagaggga
     2161 ggtgaccatg acggtgctgt caccggtctg gctggacagc cggagtcgtt atctgcggga
     2221 tatgtaccgc ccggggatgg tgatggagca gtggaacccg gagacacgca gtcatgaccg
     2281 ctatgttatc gaccgggtga cagcgcagag tcacagcctg accctgcggg atgcgcaggg
     2341 cgaaacgcag gtggtgcgta tatcctccct ggacagcagc tggtcgttgt tccggccgga
     2401 aaaaataccg gtggcagacg gcgagcgtct gagggtgaca gggaaaattc ccggactccg
     2461 cgtctccggc ggtgaccgcc tgcaggtggc atccgtcagt gaagatgcga tgacggttgt
     2521 tgtgccgggg cgggctgaac cggccaccct gcctgtgagc gattcaccgt tcacggcact
     2581 gaagctggag agcggctggg tggaaacgcc cgggcattcc gtcagtgaca gtgcgaaggt
     2641 ttttgcctcc gtcacacaga tggcaatgga caacgccacc ctgaacggtc tggcccgcag
     2701 cggtcgtgat gtccggctgt attcctcact ggatgaaacc cgtactgcgg aaaaacttgc
     2761 ccgtcacccc tcctttacgg tggtttctga gcagataaag gcgcgtgccg gtgagacatt
     2821 gctggaaagt gccatcagtc atcagaagtc cgcacttcac actccggcac agcaggccat
     2881 tcatctggcc cttcctgtgc tggaaagtaa aaacctggcc ttcagcatgg tggacctgct
     2941 gacagaggcg aagtcgtttg ctgcagaagg aaccagtttt actgagctgg gaggggaaat
     3001 caatgcgcag ataaaacgcg gtgatttact gtatgtggat gtggcaaaag gctatggcac
     3061 aggcctgctg gtttcccgtg cgtcgtatga ggcagaaaag agcattcttc gccatattct
     3121 cgaaggtaag gaggcggtca cgccgctgat ggagagggta cccggcgaac tcatggagac
     3181 actgacgtcg gggcagcgtg ccgccacccg catgatactg gaaacgtccg accgtttcac
     3241 ggtggtgcag ggttatgccg gcgtgggtaa gaccacacag ttccgggcgg tgatgtcagc
     3301 cgtgaagatg ctgccggaga gtgagcgtcc ccgtgtcgtg gggctggggc ccacgcaccg
     3361 tgcggtcggg gagatgcgca gcgccggcgt ggatgcgcag acactggcgt cttttctgca
     3421 tgacacgcag ctgcagcagc gcagcggaga aacgccggat ttcagcaaca cgctgttcct
     3481 gctcgatgag agctctatgg tgggcaatac cgacatggca cgggcatacg ccctgattgc
     3541 ggccggtggt ggtcgtgccg tggccagcgg tgacacggac cagctgcagg ccatcgcgcc
     3601 cggtcagcct ttccgtctcc agcagacgcg cagtgctgcc gatgtggcca tcatgaaaga
     3661 gattgtgcgt cagacgccgg aactgcggga ggcggtatac agcctgatta accgggatgt
     3721 ggaaagggca ctgtccgggc ttgagagtgt gaaaccgtct caggtgccac gtctggaggg
     3781 tgcatgggca ccggagcact ccgtgacgga gttcagtcac agccaggaag cgaaactggc
     3841 agaagcgcag cagaaggcga tgctgaaagg cgaggctttc ccggatgtcc ccatgacact
     3901 gtatgaagcc attgtccgcg actataccgg caggacaccg gaagtacggg agcagacgct
     3961 gattgttacg cacctgaatg aagacaggcg cgtactgaac agcatgattc atgatgcacg
     4021 ggaaaaggcc ggtgagctgg ggaaagagca ggtcatggtg cctgtcctga gcacagcgaa
     4081 tatacgtgac ggagagctgc gtcgtctctc cacctgggag aataacccgg atgcccttgc
     4141 cctggtggat aatgtgtatc accggattgc cggtatcagc aaggatgacg ggctgataac
     4201 cctggaggat gcggaaggta acacgcggct gatttcgccc cgggaggcgg tggctgaagg
     4261 cgtcacactg tacactccgg acaccatccg ggtggggagc ggtgaccgga tgcgcttcac
     4321 gaagagtgac cgggagcgcg gctatgtggc caacagcgtc tggacggtga cagcggtttc
     4381 cggtgacagt gtcacgctgt cggacggaca gcagacccgg gtgattcgcc ccggcctgga
     4441 gcaggcagag caacatattg acctggccta tgccatcacc gctcacggtg cgcagggggc
     4501 gagtgaaacc tttgccatcg cgcttgaagg cacggaaggt aaccggaaac tgatggccgg
     4561 ctttgagtca gcctacgtgg ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga
     4621 taaccgtcag ggctggacgg atgccatcaa caatgccgta cagaaaggaa ctgcccacga
     4681 tgtgctggag ccgaaaccgg accgggaggt catgaatgca gagcggctgt tcagtacggc
     4741 acgggagctg cgggatgtgg cagttggccg tgctgttctc cgccaggcgg gactggccgg
     4801 gggagacagt cctgcacggt ttattgcacc ggggcgcaaa tatccgcagc cgtatgtggc
     4861 actgccggct tttgaccgta acggcaagtc tgccggtatc tggctgaacc cgctgaccac
     4921 ggatgacgga aacggactgc ggggattcag tggtgaaggc cgggtgaaag gcagcgggga
     4981 tgcgcagttt gtggccctgc agggcagccg taacggcgag agcctgctgg ctgataatat
     5041 gcaggatggt gtccggattg cccgtgataa ccccgacagc ggtgtggtgg tgagaatcgc
     5101 cggtgaaggt cgtccgtgga atcccggtgc catcaccggt ggccgcgtgt ggggggatat
     5161 cccggacaac agcgtccagc cgggggccgg aaacggtgag ccggtcacgg cagaggtgct
     5221 ggcacagcgg caggctgaag aggccatccg tcgtgaaacg gaacgccgtg cggatgagat
     5281 tgtcagaaaa atggcagagg aaaaacctga ccagccggat ggtaaaacag agcaggctgt
     5341 cagggctatt gccgggcagg agcaggacag ggcttcgtca tcagaacggg aaactgcgct
     5401 gccggaaagt gtgctgcgtg agtctcaacg ggtgcaggac gctgtccggg aggttgcccg
     5461 ggaaaatctg ctgcaggagc gacttcagca gatggagcgg gatatggtcc gtgacctgca
     5521 gaaagagaaa accctgggcg gagactgata caggaagaca aacgctgatg acaacggata
     5581 acacgaacac gacacgtaac gattcactgg ctgcccggac cgatacctgg ctgcagtcgt
     5641 tgctggtctg gtcacccgga cagcgggata ccatcaaaac ggtggcactg gtgctgatgg
     5701 tgctggacca tgcaaaccgc attctgcatc tggagcagac gtggatgttt ctggccgggc
     5761 ggggcgcgtt tccgctgttt gcgctggtct ggggactgaa tctgtcccgt catgcgcata
     5821 tccgtcaggc gtccgtaaac cgcctgtggg gatgggctgt tgtggcgcag ttcgggtact
     5881 ttctggcggg atatccgtgg tatgagggca atattctgtt tgcctttgcg gtggcagcac
     5941 agatgctgaa atggtgtgaa tatcactcgc tggtatggtc agtcagtgca cttacacttc
     6001 tggttctctg gttcccgtta tcttccacca gttacggtgc tgtcgggatc atattactga
     6061 tgctctgtca caggatatac aggacgacgc acctttctgt acggagcatg ctggcgcttt
     6121 gtcttttccc ggtcgttgct ctcgttaacc tgcaggacag cgtgactgcc gctgtcagtg
     6181 gtgtgttagt gacatacatg gtcgtcagga tggtatccgg tatccggtgg cctgctgccc
     6241 gtttctggcc ggcagacttt ttcccggtat tttatgtctg tcatctcgct gtgctgggag
     6301 tgatggccct gtagcaggaa gcgcaagaaa gcggatgaac tgtgaaagtc tttttgttta
     6361 aatcagagcg cattcaggaa aaaacaggag cagatgatga aaaaaatatt actggtggca
     6421 gccattacgg gaatgttcgt tcagggggta tgggcaaaat cagatcagca aatccgggat
     6481 gaggctaaaa ataatgtgat cactgtttct caggtgagtg acatgacgga ggggaccggt
     6541 gtgacgctga atggtcagat agtgaagcaa ctggcagccg aagaggatca ttttgaactc
     6601 agtgatgcat caggaagtat tgtcattgat gtcgatgatg aagtatggcg cccgctggcc
     6661 ctgaaagccg gagataaagt tcaggttgtg ggagaagtgg atatttctga tgagggaaac
     6721 cggaaagcca cggtggatgt actgactctt gagcgtcttc gctgatatga acaacggata
     6781 ccggtgaggg caggtgagtt atgacagagc agaaacggcc agtactgaca ctgaaacgaa
     6841 gaaccgacgt ggaaacaccg acccggagcc ggaaaaccat catcaatgtc accacgccac
     6901 caaaatggaa ggtgaaaaag cagaaactgg cggagaaggc tgcccgggaa gcagagctgg
     6961 cggcgaagaa agcacaggcc agacaggcgc tgtccattta tctgacgctg ccctcgctgg
     7021 atgaggcggt gaacactctg aaaccctggt ggccgggatt gtttgacggt aacacgccca
     7081 ggcttcttgc ctgcggtatc cgggaggttt tactggatga ggtgtctcag cgaaatatcc
     7141 cgctctctca taaaaaactc agccgggcgc tgaaagccat cacccgttcg gaaagttatc
     7201 tgtgcgcaat gaaagccggc gcctgccgct atgatacgga gggctatgtg acagaacata
     7261 tcacacagga agaagaacag tatgcgcagg cgcgcctgga aaaggtgcgc agacagaacc
     7321 ggatcaagga tgagctgcgg gcgatcctgg ctgaataata aaaacgccct ctgtatgaca
     7381 gagggcaaca gcgaaaaaat cgggaaaaca gacatccaga gttttgatgg agaaaaacac
     7441 ccaaccgttc agggatgttt cataatgttt ctgacataat ccgggcgccg ggtcaagggg
     7501 gggataaaaa atggctgtat ccagtggatt atctgagcac agcggtattt atcgctatca
     7561 gcgcgtgggg cgtcatgccg tccgtcagga gattcgtggc tttttcaggg aagccgctga
     7621 ggcctggcag aatgcggccc ggctggcacc ctgtgaagca tggcgggatt ttgccctgaa
     7681 gagggcatct gcatgccggt gtaagtgccg ataaaaataa cccccgtcgc ggagctccgg
     7741 gggatcaggc ttatatggca gatcgttatt attattatag tggtgtaaga aggagttgcc
     7801 ctgtctttca aacggacatc acagaattaa catatttcac ctcccgggta agccagcagg
     7861 gataaaaaat gtgatgaatt tcaagaagca ggaggtgaca gtggcatgca taacagcagg
     7921 gttatccggt gaatctgaca gacagccggg ctgtattcgc ggcaggataa agaaaaaccg
     7981 ggagatgact ctcccggtcc gtcttatcgc taatcccctg agcagggcat gtatgactta
     8041 tctatgacca ttatattcag cgccacatcc ggtagcctgc gatataacac agttatcctg
     8101 tcagtaagag caggtatgat gaatacacgg agattaaaaa tataatctcc gtcacatgtt
     8161 acccccggaa accggtaacg ccgtcacggg aaagacgtga cagaaaaagc agcctccgtt
     8221 actccaggta tcagaaggct gaataatgca tgagactttc tttcttatgt gtctggtcag
     8281 gctggctgac cagcgttttt agtaacacat agccatatgt gtattaagcc acctcatctt
     8341 attttgttga attgatcaca attcaacggt ttatttttgg cgtgatgtgt gagccgttat
     8401 tcatcccggc atggaattca tgcaataacg aaaagtaatc agtgacggag agtaaaatga
     8461 agaaatatat accactggta ttatttgttt tttcatgtca ggtattttct gctgatattc
     8521 atggacgggt tgtgcgtgtt ctggatggcg ataccattga cgttatgtta tctcagcatc
     8581 cggtgcgtgt ccgtcttgtt aatattgatg cacctgaaaa gaaacaggac tatggccggt
     8641 ggtctgaaaa aataatgaaa tctctggttg cgggaaaaac ggttactgtc acatattttc
     8701 agcgagatcg ttatggtcgt attctcgggc aggtttatgc accagacgga atgaatgtta
     8761 atcagtttat ggtccgtgca ggcgctgcat gggtttatga gcagtacaac accgatcctg
     8821 ttcttcccgt cctgcaggga gaagcacgac agcagaaacg tggtctgtgg gcagacagcg
     8881 ctcctgttcc gccctgggta tggcgctcac ggaaataatg atatttctgt ggctattgag
     8941 ttctcattga aatgcgatat ttaaaagtat gaagcatatt acattattaa cttatttctc
     9001 tgttaatgaa aggtgaacaa atgaactatc aggcagtatc agaattaatc acatcgtcta
     9061 atcataatgt tcttattgtg ggggactcag cttctgaagt tgatagcttt ttaaataaac
     9121 tgaatattac tgattataag tattatgatt tcagccagat ttacagttgt tctgacagaa
     9181 cattaaatga ttatgcggtt atttttatca gggatgcact gaacgccagt gagcatatta
     9241 ttatttttaa ttgtacagga tggcctgatt tgaataatga atctgccgtc atgcagtttg
     9301 ccagagtggc acgaaaatca ggaaaacaac tgatagtcgc agtaagagaa caggatatga
     9361 aaaaaatgga agcagagttt ggaaggatca taaaaatcca ctgacgcctt cgcgttgctc
     9421 agttgtccaa ccccggggac gggaaaaagc aagttttccc cgcccccggc gtttctgtag
     9481 tgaaaagcgg gaaatggagg gaatggtcag taacagggaa tgtggtatta tcgcggcggg
     9541 tgtctgagcc tttctggttc aggcaagacg caggtaccag aaatgcgaag accccacttg
     9601 ttaatccatt aactcgtgag gcctgcatga agtacttgaa cactactgat tgtagcctct
     9661 tccttgcaga gaggtcaaag tttatgacga aatatgccct tatcgggttg ctcgccgtgt
     9721 gcgccacggt gttgtgtttt tcactgatat tcagggaacg gttatgtgag ctgaatattc
     9781 acaggggaaa tacagtggtg caggtaactc tggcctacga agcacggaag taagctgccg
     9841 ggcggggacg gaagtccccg ctttccggaa gtgtgaggta tttcaggggc agacacccga
     9901 catgccagaa accgccggtc ccgcccgggg ccggcacaca ggttaaggca tttcctgttt
     9961 ttcagccatt tcgcaacaaa aatcacatta aacaacagta atcaccgttg atttgtgcgc
    10021 caacacagat cttcgtcaca attctcaagt cgctgatttc aaaaaactgt agtatcctct
    10081 gcgaaacgat ccctgtttga gaattgagga ggcgagatgt cgcagacaga aaatgcagtg
    10141 acttcctcat tgagtcaaaa gcggtttgtg cgcagaggta agcctatgac tgactctgag
    10201 aaacaaatgg ccgctgttgc aagaaaacgt cttacacaca aagagataaa agtttttgtc
    10261 aaaaatcctc tgaaagatct catggttgag tactgcgaga gagaggggat aacacaggct
    10321 cagttcgttg agaaaatcat caaagatgaa ctgcagagac tggatatact aaagtaaaga
    10381 ctttactttg tggcgtagca tgctagatta ctgatcgttt aaggaatttt gtggctggcc
    10441 acgccataag gtggcaggga actggttctg atgtggatgt acaggagcca gaaaagtgaa
    10501 aaccccgata atcttcttta actttggcga gtgagaaaga ttaccggggc catctaaaaa
    10561 ccgtatagcc aacaattcag ctatgcgggg agtatagtta tatgcccgga aaagttcaag
    10621 acttctttct gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt
    10681 caacaaacgt attaccgcca ggtaaagaac ccgaatccgg tgtttacacc ccgtaaaggt
    10741 gccggaacgc tgaagttctg cgaaaaactg atggaaaagg cggtgggctt cacctcccgt
    10801 tttgatttcg ccattcatgt ggcgcatgcc cgttcgcgtg gtctgcgtcg acgcatgcca
    10861 ccggtgctgc gtcgacgggc aattgatgcg ctgctgcagg ggctgtgttt ccactatgac
    10921 ccgctggcca accgcgtcca gtgctccatc accacactgg ccattgagtg cggactggcg
    10981 acagagtccg gtgcaggaaa actctccatc acccgggcca cccgggccct gacgttcctg
    11041 tcagagctgg gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt
    11101 ccgaccgata tcacgttcac accggcgctg tttgccgccc ttgatgtgtc tgaggatgca
    11161 gtggcagctg cgcgccgcag ccgtgtggta tgggaaaaca gacagcgcaa aaagcagggg
    11221 ctggataccc tgggtatgga tgagctgata gcgaaagcct ggcgttttgt ccgtgagcgt
    11281 ttccgcagtt accagacaga gcttaagtcc cacggcatga aacgtgcccg tgcgcgccgc
    11341 gatgcaggca gaacccgcca ggacattgtc accctggtga aacggcagct gacgcgtgaa
    11401 atcgcggaag ggcgtttccg gggaagcctg gaggccgtga agcgcgaaat cgaccgtcgt
    11461 gtgaaggaac gcatgataat gtcgcggaac aacaactaca cacggctggc cactgcctcc
    11521 ccctgagttt tacctcatct gaataatccg gcctgcgccg gaggcatctg cacgcctgaa
    11581 gcctgtcagc gaacaaaaaa acagcaccac atacaaaaaa caatctcatc atcccgcttc
    11641 tggtgcatcc ggcccccctg ttttcgatac aaaacatgcc tcacagacgt ggaattttgc
    11701 ttatccacat ttaactgcaa gggacttccc cataaggtta caaccgttca tgtcataaag
    11761 cgccagccgt cagcgttaca gggtgcaatg tatcttttaa acacctgttt atatctcctt
    11821 taaactactt aattacattc atttaaaaag aaaacctatt cattgcctgt cctgtggaca
    11881 gacagatatg cacctcccac cgcaagcggc gggccccgac cggagccatt ttagttacaa
    11941 cactcaaata caagcaccgg gaaaaccccg gtccagcgca gaaccgaaac cgcaaagccc
    12001 ctccctcata acttaaaagc ggccccgccc cggcccaaag ggccggaaca gagtcgcttt
    12061 taattatgaa tgttgtaact atactccatc atggctgtca gtcttctggc gggaagttct
    12121 cagtacacgc tcgtaagcgg ccctgacggc ccgctaacgc ggagatacgc cccgacttcg
    12181 ggtaaaccct cgtcgggacc actccgaccg cgcacataag ctctatcatg gctgacagcg
    12241 ggtatggctt tgcagggtgg ggatgggtaa ggtgaaatct atcaatcagt accggcttac
    12301 gccgggcttc ggcggtttta ctccggtatc atatgaaaca atggagtgcc gccttccatg
    12361 ccgctggcgc ggcatatcct ggtgacgata tctgaatcgt tatatactgt gtatatacgt
    12421 agtaatgacg aggtgataaa tggcacaggt taatatgagt ttaagaatcg acgctgaact
    12481 gaaggatgct tttatggctg ctgcaaaaag catggaccgt aatggctctc agttaatccg
    12541 ggattttatg cgccagaccg ttgaacggca gcataatacc tggttccgtg accaagttgc
    12601 ggcaggacgt cagcaactcg aacgcggcga tgtgcttccc catgacatgg tcgaatcttc
    12661 tgctgctgca tggcgggatg aaatgagcag gaaggttgcc ggtaaatgat ggagatattc
    12721 tggaccatgc tggccagcca ggacaggaag cgcattcgtg agtacattgc ggagcagaac
    12781 ctgatggccg ccattgaact ggacgaacgg attggttatt cagccagcag cctggccggg
    12841 cagccgtata aaggccgcaa cggtcgtgtg gaaggtaccc gggaactggt tattcacccg
    12901 cattttgttc tggtttatga ggtcgatagc cagtggggaa aagtgtatat cctgcgtgta
    12961 ttgcatactg cacagaagtg gccatagagg gcctgtccgg atataaaaac gtctgacgga
    13021 gatgcaagaa ccatgaataa tgatgagctg gccaccagac gtgcgcaggc gattgccgaa
    13081 aacagatgtt tctcgaaagg acgtctgcgc gatgaattca gaatgaagcc tgcccccggt
    13141 gctgagccag ttaagtggta taaaaacact tatggtggca ggttcgctgt ataccgaatt
    13201 gcagactgtg ttccgatgcg tgagaaacgc ccactgacga gtaaacaact tctggctggc
    13261 cagcgacttt ccgttttatc cagacttaac agcaccagtg gccggatggc acggcaggca
    13321 tatgactggc tgagtctggc accgctcttt ctggatactg aaacgacagg actggacaat
    13381 accgcggaag ccctggaaat cggcctgacc gatgcgtccg gtcaggtggt atttgaaacc
    13441 agactgaagc ccacggtcgc catcggggcg caggccgccg ctgtgcatgg tatcagtgag
    13501 caggctctct gcggcgcacc atcatggaca gatgtggcgc ggcaactgcg gcatgcaatc
    13561 ggagatcgac cagtaattat ctttaattcc cggttcgaca tccgcattct gaaacagact
    13621 gcggccgcac atagcgatcc ggctgactgg ctggaagaaa tgacggtata ttgtgcgatg
    13681 gagctggctg caggatatta tggggccacc aaccgctatg gcacgatttc actggcctgt
    13741 gctgccagcc aggccggact gacctgggaa gggcaggcac actcagcgat cgccgacgca
    13801 cggatggcgg caggggtggt aaacgctatt gctgcatatc atctggaact gctgcaggaa
    13861 caggcccggc tgaaaatctg accgactggc ctgtataccg caatcattta cgttatccag
    13921 acgtaaatcc gccgcttgtt actgtcagta tgttaatgct aatgtatgcg tacattagca
    13981 ttaatgaggg atgatatgtg agaaataaaa ccagggaggc catgcggctt tttttaggcg
    14041 gcaggtgcta cacggctgaa aaacttgaaa aggattatct ggctgaagtg gctaattaca
    14101 gtaacgaccg ctgggaagca ccgcaacgtg ccgcacgcct ggctgccagc gtaaagcgtt
    14161 acaagacatc agaaatgctg cgctttattt ttgctaccat tgcctatgat cccgatccag
    14221 atttaacccc gctgactgtg agacgtttgt gcaaggcatt gttcggcaga accggaagcc
    14281 agtggctggt tgttgaagtt tttggggaga aggggcgtca gcaccgcagc gcggacagca
    14341 atccggagat ggtggaaaaa atggctgcca gatatcgtca tgccgccgaa ctacactggt
    14401 ccgcaacgct ggcagaaatt gaacgggtaa agcggcttta tcagacgaaa attaagaaat
    14461 ccaaaaaaga agtgggttga ctgccattgt tattcttttc cttcatcatt aatgctaatg
    14521 tatgcataca ttagcattaa tgtatggtgc gttaaacgta caataatttc gaatatccaa
    14581 actgatctca tacacgcttg taagcggccc tcacggcccg cttacgcgga ttaagataat
    14641 atttcctgtt catcagccgg cctcctgttc tccctttctt ttgtcagtgt accggtgcat
    14701 gacaacataa tcacagcatg ataataataa tcaataacaa taagctgtgt cacgtttaca
    14761 tcatggaacg gaggtgctcc tgtgaccttt gagcagaaaa aagcccgggc cattgccctg
    14821 atggacagca aaaagatgtg gcggagcaat tatgcccctc ctcttctgcg tattctctgg
    14881 cggctgggta tccggcttcc gccattaccc tttatgccgt tctggcaggt cacggtgctg
    14941 acggggggct tatggggcat ttcctgggga tgtgcaatgt ggtttatcta ggggttctgc
    15001 gcccatttcc tccaaaagtc tcactcttag cataaaaacc attatttatc atgttgttaa
    15061 ccgtcatatt cggactcaat atctccgata tcacttagtg ggtctactcc ctc
//
LOCUS       DAZFKF010000112        15021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFKF010000112 DAZFKF010000000
VERSION     DAZFKF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.114"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1550)
                     /locus_tag="ACHKA1_004269"
     CDS             complement(<1..1550)
                     /locus_tag="ACHKA1_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681839.1"
                     /translation="MLQIVGALILLITGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEAS"
     gene            complement(1671..4793)
                     /gene="agn43"
                     /locus_tag="ACHKA1_004270"
                     /gene_synonym="flu"
     CDS             complement(1671..4793)
                     /gene="agn43"
                     /locus_tag="ACHKA1_004270"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO5681840.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGIARDTLIYAGGDQSVHGRALNTTLNGGYQYVHKDGLALNTVINEGGW
                     QVVKAGGAVGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVTGINRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNVGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNNTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQSN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGGIVRGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVY
                     GAAGHSSVDVKDDDSSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNHDFRARGRGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGKDNAGYVKFGHG
                     SAQHVRAGFRLGSHNDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYVHSVSGSSAEGY
                     NGQATLNVTF"
     gene            complement(5166..6038)
                     /locus_tag="ACHKA1_004271"
     CDS             complement(5166..6038)
                     /locus_tag="ACHKA1_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO5681841.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHTGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDS
                     VVSVARAVWNWIFF"
     gene            7268..8752
                     /locus_tag="ACHKA1_004272"
     CDS             7268..8752
                     /locus_tag="ACHKA1_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO5681842.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PNESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(9074..9676)
                     /locus_tag="ACHKA1_004273"
     CDS             complement(9074..9676)
                     /locus_tag="ACHKA1_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681843.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLMYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(9763..9969)
                     /locus_tag="ACHKA1_004274"
     CDS             complement(9763..9969)
                     /locus_tag="ACHKA1_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5681844.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            10728..10877
                     /locus_tag="ACHKA1_004275"
     CDS             10728..10877
                     /locus_tag="ACHKA1_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO5681845.1"
                     /translation="MEKVISHTRYKLTSAELEAFNSVVDHWLAELTMNKLYDRVPASV
                     WKYVI"
     gene            complement(11292..11492)
                     /locus_tag="ACHKA1_004276"
     CDS             complement(11292..11492)
                     /locus_tag="ACHKA1_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681846.1"
                     /translation="MVIRKKKCWDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
     gene            11697..12266
                     /locus_tag="ACHKA1_004277"
     CDS             11697..12266
                     /locus_tag="ACHKA1_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO5681847.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGIIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERSGYLAKEHTKARGTGERNFGCSRS"
     gene            12527..12904
                     /locus_tag="ACHKA1_004278"
                     /pseudo
     CDS             12527..12904
                     /locus_tag="ACHKA1_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
     gene            12908..13333
                     /locus_tag="ACHKA1_004279"
     CDS             12908..13333
                     /locus_tag="ACHKA1_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO5681848.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLSVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            13580..13777
                     /locus_tag="ACHKA1_004280"
     CDS             13580..13777
                     /locus_tag="ACHKA1_004280"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO5681849.1"
                     /translation="MPQDDFCIVNPGGCIVHFPANGRDIQDINAPDFPEQYQRLLARL
                     DYLTKPDAKASGQRNSGYSGF"
     gene            13805..14371
                     /locus_tag="ACHKA1_004281"
     CDS             13805..14371
                     /locus_tag="ACHKA1_004281"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO5681850.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDQSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLSEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESYGAIERHLGCSDF"
     gene            14427..14691
                     /locus_tag="ACHKA1_004282"
                     /pseudo
     CDS             14427..14691
                     /locus_tag="ACHKA1_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991605.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14757..14954
                     /locus_tag="ACHKA1_004283"
     CDS             14757..14954
                     /locus_tag="ACHKA1_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5681851.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
BASE COUNT         3554 a         3711 c         3932 g         3824 t
ORIGIN      
        1 gacgcttctg cgggatcggt tacaccgggt actttacctt cgcggatctt attcctcatg
       61 gtttcaacag cgccggcata ctgatcacgg gggacttcca gttgcatggg gccattattt
      121 cccatgtaac gatactgtcc gttttgtccg tcgaatgccg caccgacgct acgggctgca
      181 gttgaacaat atttcgtctg gatttctgtt ccgctgacca gtctgtctgc tccatttctg
      241 gcattattat caccgaggat ccgtgcgttt ttcagacgga ctctgtcgac aatatggttt
      301 ccccgttcag cggcaaaccc atgtccctgc cctccggaag caaacatttt ctcatttgta
      361 ctccaggttt ccggcagttt catgagaatc agaccattcg ccgttgcatc ggaaataaag
      421 ctgttgtaag ggcgttcata aatttcctga cacagtgttt ctgtttcgcg acaaagagag
      481 gagcagtacc attccggatc cggcttatga ttttccgggg catgatgtac ttctgtcagc
      541 ccgcaggagg cacagatacg aagctcatcc acgtgatgga atgatacaaa acggcgacct
      601 tctgaaaagg gataccagtc accgccgtcc aggcaacgat cgagaaaatc gtcattcgct
      661 tccggtgata aattcaggcg gttccggaag agcgttttca gttcatcagg tgtgaatgac
      721 aggttctctt tcaactgttc gttaaaccct gccatgtttc tttcatacca ggcattgttc
      781 agtaaccagt ttccgtctgc cagttcaaat tccctcagct ctccccggga aatccgttga
      841 gaaatgagcg tcagccagat gcgctccgtc agtgagtcag gaaggctgta atcgttttgc
      901 aaactggtca gccacgattt actgttgagc accggttgtt cttccagtgt ctggctgatg
      961 gcacaggtca gttttttcag cgcctggatc tggctggccg cccatagcag cgtcactaat
     1021 gcagccagcc agtacaacaa cccgccccac cacatcagtg acggtgaaat atcgtactgt
     1081 aacgcgatca atggtgcgca tgcagaacac gccataagga aaaaggcagt ataaaaacag
     1141 cagtaatacc cgttaatttc cttgctgata tcttctggtg tgatcccgtt ctgctgaatg
     1201 tttttgatct gcaggggaac agccagcaga ccaccggcga gagcagtggt cagaataaat
     1261 ttcacagagt caaaggtgtg agcctccagc gcgatatgtt taccatcaag tccccacatg
     1321 aagctgatga tcattccggc aatcgtcaga aatacgcctg ccagccagcg aatatggaac
     1381 aaccgggtta tgcgttcggt gatatagcca gccagtaagg ccgggccgaa cagacacagc
     1441 aatatgagcc ctgccagcgc agaagccgtg ctgattaatg ctctgaacaa aaggcgaaga
     1501 atggcaaatc ctgtgatcag caggatcagc gcgccgacta tctgtaacat agtcactccg
     1561 tgatgaatat catgttattg ttaatgccag cgaatgtggc gttgaatatc cgttgcgccg
     1621 gtccgggtcc cggtcatgat gaccgggacc acagagaggc gatggttctg tcagaaggtc
     1681 acattcagcg tggcctgacc gttatagcct tcagcgctgc tgccgctgac gctgtggaca
     1741 taaccagcct gaacgcccag ggtgatgttt tcccggacac gggcttccag tccggcctgc
     1801 aggtccagtg acgtaccatt ctgtgacggt gagaacgtca tgttactgcc ggctgcggct
     1861 gtacccatgc tcatgtcccc ccgggaactg aaggtgcgga taacagaagg ctgtacccac
     1921 cagttcaccg gcagttcacg cacactgtgt ttggcactgc cgcgcagggt gtcacgggat
     1981 gaggtgcctt taccaaagtt catatcgttg tggctgccca gacgaaatcc ggcacgcaca
     2041 tgttgtgcac tgccatgccc gaacttcaca taaccggcgt tgtccttacc gtcatccagg
     2101 gaaagtccct gccaggtgta ctgcagttgt ggctccagca tcaggttgtc agtgatactg
     2161 aagggcagac cggtttccag tgagcccagc cagccccggc cccgtgcgcg gaagtcgtga
     2221 ttgtccgatg aggctttcat gctgtggcgg gttccctggg ccacaatgtc agcccacagt
     2281 ccggaggcgt tgtgtatcag attcaggtat ccgcccaggc tgccggcatc atcccggacc
     2341 gtgccggcac gggagctgtc atcatcctta acatcaacgg aagaatggcc tgcagcacca
     2401 tacacccctg tcgtcagaga cataccggcg acctctgttc tgagcaggtc accctcaaga
     2461 cggacgaagc catagctgcc gctgctttcc ggcgtggccc cacggacaat accgccgttg
     2521 ttatcgtgac cgagatggcc gccctgaatg ctgagacgga cgctgttgtt ttcaccattt
     2581 acaccggtct gatggctgcg ggagcctgcc agaatccggt catagtccat tgcctgtgtc
     2641 agcatggagg catacagggg gacttctgca cgataagcat tttcactgcg cagataccag
     2701 ctctcatcac tgtcccggtt gagggagtag ttaaaggccc cggcctgcag cctgttcccc
     2761 tggacaaagg ccccttcctc cgtggtggca ccgttaatgg cttccaccac ctgaataccc
     2821 ttaccgctgg tcgccagccc cgacgcactg ttgccggcgt tcaccaggtt caggatggtt
     2881 tttccggttg ccctgccgcc gtcaatgacc agtctgtcag cattgctctg tgccatatcc
     2941 gggcgtacac gcaggctgat ggtgccattc tgcccgttca ggtttttcac tttcagggtt
     3001 gccggtacga acttccctgt gcgggtggag gtgaaatgaa tctgtccggc atggctgagg
     3061 tcatccacca ccgactgcac cgtggcgtta tcagggatat tccaggtggc accggaggcg
     3121 agagtgacat tcgtggggtc aatggcaccg ttcagcacag tgctgccggt cagcttcagg
     3181 gctgtaccgc gctgagcaat gacatccgtg gtgacggtac tgtcgttcag cgtcagcgtg
     3241 ccttcattca ggttgacggc tttctgggtg agtgtggtgt tgctgacagt gagtgtgcca
     3301 cttcctgatt tttccacgct gccgttaccg gtgagagagc ctccctgcag gagtgcatcg
     3361 ccttcacgga tggtcagggt attgttgttc accgtcttcc cggaaagggt aagtatggcg
     3421 ccgttattca gcgtggtggt gcccgccagt gtgccgcccc tggcggtgaa cagtccgcca
     3481 tttaccgtgg tatccgtggc cgtatcaccg acgttcagcg tgaatgaact gcctttttcc
     3541 agcatcaggg catccgcctg accgcctccg atactgaatg cctttccgtc gctccgtgta
     3601 ccactgacag cggcaccgga atcggccagc agtacaccgc catttcccat ggatacggtg
     3661 gtggcggtac caccgttgcg gacatccagc gttccgccat catccacgcg ggtattagtg
     3721 gctgtgtgtc cggtcagcac atccaggcgt ccgccatttt ccagtacgac attatcagct
     3781 ttaccctcca caaccgagaa tgctccaagg cggtttatgc cggtaacggt tgcagccgta
     3841 ctggtaacca gtgcaccgcc cgtgttctgg gtgactgcag tggcttcccc gccggcatgt
     3901 accctcagtt caccgttctg atttatggtg gtgttaccga cagcaccacc tgccttaaca
     3961 acctgccagc ccccctcgtt aattaccgtg ttcagcgcaa gtccgtcctt gtgcacatac
     4021 tggtaaccgc cattcagtgt ggtattcagg gcccttccgt gtaccgactg gtcaccacct
     4081 gcgtaaatga gagtgtcacg ggctatcccg gaaaataaga taatctgccg tccatttttg
     4141 ttgatggtgg tggattctgc tgttccctgg accttctgac ccgtgtacga gttgtcagaa
     4201 tccgggccgc cttctgcgcc ggtgttgatg atggttcctg ttgccagccc gccactttta
     4261 acgctctggt aaccgtcgcg gttgataatt gtaccggtgg caaccccgcc ctcatgaacc
     4321 cactgctcgc ctctgttatt cagcgtggtg ttcaccgcca gcccgttcag gctctgtccc
     4381 ccgccgtcac gaataaccgt atcactggct gttcccccct ccagcacgac ctgacgacca
     4441 tttgtggtga cagtggtgtt tccggctatc ccgccattct gtatccattg cccaccggtg
     4501 ttttcttcac tgtccggccc cagttccagc ccggtactga tggtcattcc gttggccgta
     4561 ccgaagacaa tctggttgtc atgatttgtc agtgttccgt cgttcacggt ttctcccgcc
     4621 tgtacaaccg tgtcagcagc cagtgccggg actgatgtga cagcagcaag agacagcgca
     4681 accgccacac cggcgcgttt tccccgtgag cgggccagtt cggaggccac caccagggtg
     4741 cccgtaatgt gattccatac cagcctgtag ctggtgttca gatgtcgttt catcagcttt
     4801 tccttacaga gggtgaataa aaaagccggt acccacaaat gtggataccg gcaggtacaa
     4861 aacagcatgg tcagataaag tggtttttcc cggacataaa tgcttcccct ctctcatccg
     4921 ccagagaagt atcagtccgt tgtgtgcaat tccgttctgc atgatgaaga gattcagctg
     4981 ccttttcgca gaccattaac cactataaac gatcgatagt aacgatcgat acctgtttta
     5041 tcgatcgttt tattctattg acagcagagc ggtcacgcaa gagaaacagg agggggatga
     5101 ggggtttgtg tcatgttttc agttggttaa cgcaatgtaa tcaggcattt cccctggcag
     5161 agtgttcaga agaaaatcca gttccataca gcgcgggcaa cagagaccac cgagtcacgg
     5221 acggcacgca gaaccgtgcg tgcaacagag gcaatacaga cgctctccgc cgtgtcaaat
     5281 atccggtcca ccgcaccggt aaactgttca cgggcctgag accggacaga ttccgtgcgc
     5341 agctcgtcct gcagtcgggt catcagggga ctggcggcat ggtcgggaag cgctgtcatg
     5401 agcgcactga ccagcgtatc cagttcccag ccggtgcggg ccgatacggc cacaaccgga
     5461 tgtacgggcc ggaacagacg gaataccgcc tccgtttttt cgcgaatatt ctgtgcctgt
     5521 gcaggagaag gctgaatacc ggccatatcc cattcatggc agggctccgt tttgtcggcc
     5581 tgcgtcacca caaacagtac ccgctgatgt ccccggtgca ggatgtgtcg ccagaaatac
     5641 tcatccacag acagggcacg gtcatcggct ttaatcagcc acagtaccag gtccagttca
     5701 ggcagaatgt cacggtacag ggcctcatac tctgcatccc tgtcccggct ctcgcccacc
     5761 ccgggcaggt cagtgataac catgctgtga ccgtggccac tcagacggaa gcgccgtact
     5821 tcccgggtgc cggtgtgaac atcactgacc ggggtgacct ccccctgaaa cagtgcatta
     5881 cagagtgagg atttaccggc cccgctttta cccataatgc caatcactgg ttcgtgacgg
     5941 gtgagtttgc gcagatgttc caggatgtga cgggaaagcg agtaaggcag ggaggagagc
     6001 ggtttttcaa ttgcctcaat ggcatcagac ggattcatac atgtattccc ggtgaaaaaa
     6061 gacaaaaccc cgcgcaccgg aaaggtggcg ggggattcac ggagataata tctgtctgaa
     6121 attgttttga atcgatggga ttgttgcgta ttaacatgaa ggagatcagg acttatccgt
     6181 tactgggtga tctccttcca tgaaaatacg ccgttattgt taaacagcag gctgatttct
     6241 ccgtaaaggg gaaaatattt atcggctgca tgattatcag gtgtgtctgt gtttactgct
     6301 gtgagtattt ttcagtgtat tgtgggggag atgtcagtgg gagatattaa atttacgttt
     6361 ataattatct tacttcttct gtgtgttttt tataagagtg cacacgtaat gatatctgtt
     6421 gataccatgt ggttaagtgt ttcccttcct ggtcatgcgt aatgattaaa tgcttttgtg
     6481 acaccattct tccggtctga atatttatcc agttcttttt tatttgtgca gtgatactgt
     6541 cggaagagaa ttcagtaatg tgaggtgtcc tggtcaggat aatatctgct gagatattat
     6601 tagttgagtg tgtttttact gagcaaaagc tggcttttaa ttcctgtact gaaacgctgt
     6661 cgctcgtccg gggttaactg aaactggatg atgatgtaaa atgaaggctg tttttgtgtt
     6721 tgtcgaagaa gtgttatttt aatccattct catcattggc tggcgattat acagaagtat
     6781 ttgaagaaaa aatggtgcat tttatctgcg tcagcaggga ggaagtattt tattgatgta
     6841 tcagtattaa atggtttttg ttatttataa atatgtcatc ctgtccatag aatattgtac
     6901 tcagcattca gtgcaggaac tgtagtcttc tttattttat tcttcttcag gaggggggga
     6961 ttcctgaagg cgttattccc ggtgttctgt ttatgattct tcccggcagg aaaccggtat
     7021 ctgcaatacc cgggatgatg ctttttgttc gtacgtggtt gtgtaaaatt tgaatagtgg
     7081 atgtgctctg aagggaagga ggggacacac agattcattt tgtctttctt cagcagcaaa
     7141 acacactgtg gttattattt aaccattaaa gcaacaaaaa tttttacctc atattgattt
     7201 gactgctatt tttgctatta atagccagaa ttgcagtact tttatatctg tgtgtagtga
     7261 ggttactatg tcattcggtc agccttgtga tgagtttccc ctgagttcgc tgcctccact
     7321 cattcgtgat gccgttattg aagctcagca aattacgcag gctccccttg ggcttgtggc
     7381 agcgtctgcc ctgggcgctg tttcacttgt ctgccagaat ctgattgatg tctgcagact
     7441 gaatacactg cgggggccgg tctctctttt tttcctgacg cttgccgagt ccggagaacg
     7501 aaaaaccgca gtggacaaat tattaatgaa gcccctgtac cagcaggaga tgcagttata
     7561 ttccagatac aaaagtgaac tggctgtctg gaaaaacaag gaagagcttt taaaggctca
     7621 gaaaaaagca ttgttgtcaa aactgaataa agaactccgc aaaggcgcgg atgagtcaga
     7681 aaccctcaga caactggaag tacttcagaa aaacagtgcg gaagaacctg tgaggtataa
     7741 attcattttt aatgatgcca cgactgcggc aattaagaat cagctttgtg gtaaatggcg
     7801 ttcagtgggt attatgtcag atgaagccgg tattatattt gatggatata ccctgtctga
     7861 gttgcctttt attaacaaaa tgtgggatgg ctctgtattg tctgtggaca gaaagaatga
     7921 acccgaacag atgattgaaa atgccagaat gacgttatcc ctcatggtgc aacccggtct
     7981 ttttgaccga tatatggagc gaaaaggttc tgttgccagg gacagtggtt ttcttgcccg
     8041 ttgtcttatc agtaaacctg caaccacgca ggggaaacgc tttattaatg gagctgtgat
     8101 tccgggagga tcattaacgg catttcatga acgattaatg gaactggcca gaggcagtat
     8161 tgaaaaatcc agtgaggatg aacggtattg tctgcatttt tcgcctgaag cgcaaaaaat
     8221 atttattgag cactataatg ttctggagca ggatttatcc ccttccggac ccttaagccc
     8281 gtttcgggga catgtttcaa aaaaaacaga aaacattgca agaattgcag cgttatttca
     8341 gtatttcagc tatggtgaag ggaaaatctc tgccgatatc atgacgtctg ctgtcgtaat
     8401 aagttcgtgg tacacagatg aatataaaaa attatttgcg ttacctaatg aatctgaact
     8461 gcagcagaag gacgccgagg agctgttcga ctggctcatt gaggagtgcc gcggagaatg
     8521 cccgccccgt gtcaggaaaa attatattct ccagtgtggc ccgggccggt tcagaaacag
     8581 gaaaaaactg aacgcattgc tgaacattct tgaatctcag ttcaggcttt ctgtcgtgcc
     8641 ggaaggaaaa accatgtatg ttctgcttcc ccagatagcc agtttaaagt tgtctgatgt
     8701 ctctggtatt tttacctcgg gttatcacta caataagctg agagcgaagt aaccggtgag
     8761 cagtttttct ccattatgct tcagacttag atgattgatt attatgggga tttcatcacc
     8821 gatattcaga tgtatgaata tcggtgatga actaaagctc aggaactgcg tggtggtgat
     8881 gaatcaacag gcatataact gatacccgcc ggtcagaagt tccagcctgc gtctttcctg
     8941 tctgcgtgcc cgcagtccgg cgttcaggtg cagcgtgtga cagaacgcac ggagagcgaa
     9001 acggcaggca gacaggccgt tcagtcagcc ctggcaccct gcccggatac cagcttccct
     9061 gaaacaactg gcatcaggtg cgcttatcgt caaccgttgt gcggtatgcc aggagcacgt
     9121 actgatccat cagatccgtc agtgctgcgc tctgcttctg acggttgatg atccattcct
     9181 ccagctcttt ttctgatttc cgtttcagat tctccgtcca gtcgtcaagg cagtttccgg
     9241 tcattaaatc aagccagcaa agccagacca gtttgaggcg caactccgtg ccaccaaact
     9301 gacggaagag gctgatcagt ttatttgcca gcgaacgctg cctcagacgt tcacattcag
     9361 acaggagaat gccttccagc atcggatgat gaaccagcag atgttcactg gtgcataaat
     9421 tcactatcag atcatcatcc aggggcggcg tggtccgttt gtccggacca tacatcagcc
     9481 ggtgcagact ctggggtacg atggcaatct ccggtttttc accattgaat gacacccacc
     9541 cgggcgtcag attatggaag tagtagatat tcggatgaca cacgccccag gatacggcgt
     9601 gccgggtgaa gccggtcagc catttctggt agctttcgtg gagcagctcg taacgggtct
     9661 gatactgtgg tttcattttc atattcctca cgggggataa caggcagcag aacgccgccg
     9721 gcaggccgga gactgcgtct gctgcagatg aacggttaag aattacggac gggactgtgc
     9781 aatacgcgcc tgcagccagg cttccacttc gcttttcagc catcgggagc tgcggcccat
     9841 tttgatgggc gcaggaaagt caccatcctt gatgagtttg taaaaccact tatcggtcag
     9901 gccggtcagt tgagtgataa atgccatgtc gaccatctgg tcatccatca gtgaaactgg
     9961 ggtgatcatg agaatgttcc tccggatgtt gttaaaccag ccggtgtggt catgaaaaca
    10021 acgcaccacc ttcaccggca taacaaccgg ggagaaacgt aaaaaataat cgggaatgtg
    10081 accgccagga gataaacggg gaggttacgg tggctgagcc gtaccggtat gccgggtggt
    10141 tgttacgaaa cgatgcttcc gcacgcgcgc gcgataaaga agggatgatt ttcgtgagga
    10201 agaggggaaa actggcgttc tgataattgc agagttattt acggggcggt tacgatgggg
    10261 cagaccgtgt ccggtgggca ggtgacaggt atcaccgatg ggataagact gtctgattgg
    10321 tattataaat atataagaga gatcaaacac atgagatcaa atacatgagt tcatatatgt
    10381 gaaataaaca cggtgagtgt gatcagtggg catgatgaga acgcagtagc aacagactgc
    10441 gctatccctg atagctggcg tataacccgc gtatataaaa cgtatctgca atgtacgtga
    10501 acagagtgca ttaagcatcc gtctgagcgg taaacatatc cgacctggtg agtgacagtc
    10561 tgagagaccg gttaagtggt ggctcgggag taatgagttt ggggtgggta ccagaatgcc
    10621 agtctggcgg tatgctgaca gtatcacgtt aattgcagca taaagtgatg agaggctaat
    10681 ggaagttaaa accaaagcag ttccgtcgtt gttcatcccg ggagacgctg gaaaaagtga
    10741 tttcccacac gcgttataaa cttacctctg cggaactgga agccttcaac tctgtggttg
    10801 atcactggct ggcagaactg acaatgaaca agctttacga tcgtgttccc gcttcagtct
    10861 ggaaatatgt catctgagcg ttcggtaatc acaactgcat cgcgccaggt gttatccgct
    10921 ttgtgattct tattggctct tctcaggtta ttaaaagctg aatatgttcc tgttgtcggg
    10981 aaccaggata ataccgggaa cgttaagcgg tatagcgaca acaaagaatt cgaaatctaa
    11041 agggaatggt gccggaggcg tgggcggtaa tgggctgatt ttcaggtatt accggcttac
    11101 agcgtgtggt tgatattgag gggctgtgag tttcacgtat ttgtcagaaa cccagacgag
    11161 ttttcctgta tgagacgaaa taaggcagcg gcaatacctg gaaaaattta atatggtatg
    11221 aaggagaacc tgtcggttac gcttaatata cccggtacac tgtcagctca ggggatacat
    11281 gaaccgttat ctcaccagct acacagtata tacacactga cgctgacagt agtcagcagg
    11341 accacaataa tcagtgccag cagtatagta acgatacggt ctgtattccg gtaatagccg
    11401 aaggggtcga cagaaccaca atacgggcaa cacacagtat tatgagtaat cgcgtttcca
    11461 cagtcccagc acttcttttt gcgtatcacc ataactccct cctcacagcc ttatccgtaa
    11521 tcggtttttt acattaaaaa aacaacgctg aaaattcaac cgtcagtccg gagacgaccg
    11581 ttcgggttat ctcagagtgc ctgagatatt gcctggttgg agatcacttc agggactctg
    11641 cgcatttttt acggcgtggt taatccgtat gaacgaaacc atacggagat ttaaccatga
    11701 cctacaaata caaccccttc tggcagcaac gtattcgtga gacggtgcgg cacgcactgg
    11761 atgttcatcc ccgtctgacg gcattgcggg ttgatctgcg tctcccggat gtaccggcag
    11821 caacggacgc ggctgtgata tcccgcttca tcaatgccct gaaagcccga atcgacgctt
    11881 accagaaacg taagcatcgg gaaggtaaac gcgtgcattc ctcgaccctg cattacgcct
    11941 gggcccggga gttcggggag cttaaaggca aaaaacacta tcacctgctg ctgctggtca
    12001 accgggatac ctggtgtcgt gccggcgatt accgcgctcc ggggtcactg gccgggatta
    12061 ttaaacaggc ctggtgcagt gccctgggag tggatgccgg acgttatgac acgctggcac
    12121 attttcctga caagccagcg gtgtggctgg aacgcgatga tgaaaccggc tttcagcagg
    12181 tgctggaacg ttctggctat ctggcgaagg agcataccaa agctcgcggc accggtgagc
    12241 gcaattttgg ctgtagccgg agttgaacac gacaggcgca ccaatacccg gcaaaccaaa
    12301 agtatgggtt gttgtctggc ggcaacgatg tatccactcg cccactgact gttttttacg
    12361 tcagtgggcc attgtgtgac aaccacagtg ctgtaacagc aaccagcctg ctttacccaa
    12421 actccagtcc agattttaac tgaaccgcca ttcacaccct gaccacgctg cccgtacctg
    12481 gacggctgtt gcctgcgtgt cgtctggcgg ctgaggaata tatactatgt acgcaaaatc
    12541 ctttctcgct cttgatggca acggacgtct gacgggggcc cgtaccgcac agactgcgcc
    12601 ttacgaccgc tacacctgcc acctttgcgg cagtgcactc agataccacc cgcaatacga
    12661 cactgaacgg ccctggtttg aacacgctga cgaagggctg acggagcacg cgcagcaatg
    12721 tccttatgtc aggccggaac gcagagaaat acagttgatt aaacgtctgc agcaattcgt
    12781 cccggatgcc ttacccgtgg tgcgtaaagc cagctggcac tgcagacaat gtcaccacga
    12841 ttattatggg gagcggtact gcacacactg ccagaccgga cgcttcagtg aggagggggg
    12901 tagcagaatg aacggtataa aaacggggct ggggatcacg ccgggtgaac acattatcag
    12961 tgccgactct gcgctcagca gaaatatccg gcattgtttc tgcctttcct gccatggccg
    13021 gttgattctg cagacagatg ctcagggggc atggtttgag catgatttac atgccctgag
    13081 tgagcaacag aaggcggact gtgtggtgct gaatccggaa aagtcccacc cttacattga
    13141 ggatatggcg atgtttttat cgccattatc ggtcgtgctg gagtggcatt gtgtgatgtg
    13201 tgagcagttt tttcatggga agaagttctg tgaggcctgt ggtacgggga tttattgcag
    13261 agcggtatgt acgaagtctg tttacagcta ccagccagac ttgtttgagg actgcggagg
    13321 tgcgagtact taatccgaaa tccccgctct gcgcagggcg ttcagaggta ttgccaacct
    13381 gtattttatc cggtgtttcg cacccacccc gttcgtgaca caacggcgcg acaccggata
    13441 tccgcagtcc tgcttacctg aataaaaccc ggggtattga ttggtagtgt ggtgcggaat
    13501 cgtaccgtga tctcgcatcc gccgaattcg cactgtggtg tcaatcaacc atacgcgaga
    13561 gtcctctccg tatttatcag tgcctcagga tgatttctgc atagttaatc cagggggctg
    13621 cattgttcat ttccctgcta atggacgtga catccaggat attaatgctc ctgatttccc
    13681 tgagcagtat cagcgcttat tggcacgtct ggattatttg acgaagcccg atgctaaagc
    13741 atcaggccag cgtaactctg gctacagcgg tttttgacct gttgtaactg taaggtgatt
    13801 tattatgtat acagaatcaa atgttaagct ttcctgtaac gccctctggc aaaagtgtgt
    13861 ccttgcaact ctgtatcggg ctgcagccac acatcctgaa attaaattat tacaatttaa
    13921 attaatacca gggcagggta ttaataaggc cattccctct ctgatggctg attttactgg
    13981 tgaactgcat agactcattt ctgaatattt gcagtgtaag cgacgtaaag atcaatctgt
    14041 tgagcccacc cctttatatt atctctggag tagggaagct gtggctaacg ataccactcc
    14101 ttatcttatg gtgctgctac ttaataacga tgtctggtgt aagagtcgtg aggtggtggt
    14161 tctaagaaat ctgattgagc aggcgtgggg aaaagtattg agtgaagata actccattac
    14221 acatctggtt gtgtatcctg aagtgttgtc gttggtattt gatgcagcta acacatacaa
    14281 cgctgagcct ggtgtactgg aacagacggc ccgggcttac attgaatcct atggtgccat
    14341 tgaacgtcat cttgggtgta gtgactttta acgtcaggtg acgcattgtc acttcagacc
    14401 tgctacctgt acgccggaat ataatggtca gagaggcata tttcgacgcc cctaatctgg
    14461 ggcgtttgat tcaggatgtc tggaacaatc cgggctttgt gtcaccgggc gcatcaaagc
    14521 tgtaccaccc gggatgtggc gctggatcgt catttaccgg actgcgaaga catacttaaa
    14581 gaggtgatat gggcgttcag tgattttgtc cgcgataatc gtggtgtgta tgatccggaa
    14641 gcccgttacc ccgccggtaa tccctggtat cccgtaacag gccagttctg aaagcacagc
    14701 tcagcagatt ccccgcattc gcacaatctt cataaatctc acaggacatt ctgaccatgt
    14761 tgactacaac aagccacgac agcgtatttc tgcgtgccga taattccctg atcgacatga
    14821 actatatcac cagtttcacc ggtatgaccg acaaatggtt ttacaagttg atcagtgaag
    14881 gccatttccc taaacccatc aaactggggc gcagcagccg ctggtacaaa agtgaagtgg
    14941 agcagtggaa gtgaagtaga gcagtggatg tagcaacgaa tcgaggaatc ctgggggcag
    15001 cagcatgaaa cgtgttgtaa t
//
LOCUS       DAZFKF010000113        14385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFKF010000113 DAZFKF010000000
VERSION     DAZFKF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.115"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            62..418
                     /locus_tag="ACHKA1_004284"
     CDS             62..418
                     /locus_tag="ACHKA1_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5681852.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(469..1365)
                     /gene="yhaJ"
                     /locus_tag="ACHKA1_004285"
     CDS             complement(469..1365)
                     /gene="yhaJ"
                     /locus_tag="ACHKA1_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO5681853.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFNGK"
     gene            1470..2171
                     /locus_tag="ACHKA1_004286"
     CDS             1470..2171
                     /locus_tag="ACHKA1_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO5681854.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLRKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2194..2358
                     /gene="yhaL"
                     /locus_tag="ACHKA1_004287"
     CDS             2194..2358
                     /gene="yhaL"
                     /locus_tag="ACHKA1_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO5681855.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2492..3802)
                     /gene="cyuA"
                     /locus_tag="ACHKA1_004288"
     CDS             complement(2492..3802)
                     /gene="cyuA"
                     /locus_tag="ACHKA1_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO5681856.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVVEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3830..5161)
                     /locus_tag="ACHKA1_004289"
     CDS             complement(3830..5161)
                     /locus_tag="ACHKA1_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5681857.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5436..6800)
                     /gene="tdcG"
                     /locus_tag="ACHKA1_004290"
     CDS             complement(5436..6800)
                     /gene="tdcG"
                     /locus_tag="ACHKA1_004290"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO5681858.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6872..7261)
                     /locus_tag="ACHKA1_004291"
     CDS             complement(6872..7261)
                     /locus_tag="ACHKA1_004291"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO5681859.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7275..9569)
                     /gene="tdcE"
                     /locus_tag="ACHKA1_004292"
     CDS             complement(7275..9569)
                     /gene="tdcE"
                     /locus_tag="ACHKA1_004292"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO5681860.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9603..10811)
                     /gene="tdcD"
                     /locus_tag="ACHKA1_004293"
     CDS             complement(9603..10811)
                     /gene="tdcD"
                     /locus_tag="ACHKA1_004293"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO5681861.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10837..12168)
                     /gene="tdcC"
                     /locus_tag="ACHKA1_004294"
     CDS             complement(10837..12168)
                     /gene="tdcC"
                     /locus_tag="ACHKA1_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO5681862.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12190..13179)
                     /gene="tdcB"
                     /locus_tag="ACHKA1_004295"
     CDS             complement(12190..13179)
                     /gene="tdcB"
                     /locus_tag="ACHKA1_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO5681863.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVARPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13278..14216)
                     /gene="tdcA"
                     /locus_tag="ACHKA1_004296"
     CDS             complement(13278..14216)
                     /gene="tdcA"
                     /locus_tag="ACHKA1_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO5681864.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3723 a         3854 c         3355 g         3453 t
ORIGIN      
        1 aatagcattt acacaatacc cggaatagac tttcgtcact tttaattaaa gggatgtttt
       61 tatgcagtgg tatttatccg tattaaaaaa ttatgttggt ttctctggtc gcgcacgtcg
      121 taaagagtac tggatgttta ctctgattaa cgccatcgtc ggcgctatta tcaatgtcat
      181 tcaattgatt ttaggtctgg agcttccata tctgtctatg ctttatttgc tggcaacctt
      241 ccttcccgtc cttgcgcttg ctatccgtcg cttacacgat accgaccgtt caggtgcctg
      301 ggccttgctc ttttttgttc cgtttattgg ctggctcgtg cttctggttt ttttctgcac
      361 agaaggtact tctggcagca atcgttacgg aaacgatccg aagtttggtt caaattaatc
      421 ttagaattgg ggcgatattt cgcccctttt tattaacaat aataaatatt attttccgtt
      481 aaaaagtttg ggaatttccc gcagacacca ggatttcgct tcccccatac tgtcacgacg
      541 ccaggccata ataatatcga tctcgctggt cgattccggg ctgacgacac gcaaccgccc
      601 ttccgcaata tctttttcga ccatcggata cggcatcgtc gccacgccaa gccccgccag
      661 taatgcctga cgtttatctt caatcgtgct caccgttaag cgcggctgtt tgtccagcag
      721 ctgtacggtc aacaccgggc gctcacgagc ggtatccgcc accgcaattc cacgatattt
      781 cacacgcgtc acttcagata acggttccgg ctcctgatga atcgggtgat caggcgcggc
      841 aacgtagacg ttcattaacg tatagagctt gcgcgagttg atctccgacg aggaacgaaa
      901 atgcatatcc ggcgcgataa caatatccgc ccgcccctgc tccagccgtt cccacgcccc
      961 cgccagcact tctgtgatga ttgccagttg ggtattggct tttgccgcca gtttgtcgat
     1021 taacgggaaa aaggcaggtg tcggtaccag cgcttcggtc acaatggtga gatgcgtttc
     1081 ccaaccgcgc gcgagagctt ccgcatcagt agtcagttta tctgcggctt ccagcaaaac
     1141 gcgccctcgc tccaacaaca tcctcccgac attggtgaat ttggtgcgat ggcccgagcg
     1201 gtcaaacagc accacatcca gctcttcttc cagtttttgc atggtgtagc taagtgcgga
     1261 aggcacgcgt cccagctcat ccgctgccgc cgcaaaactg ccccggcgat cgatcgcatc
     1321 cataacccgt agtgcttcca gcgttaatgc cctttctttg gccatttcgt tctcattcag
     1381 gaaatttgaa cataccgggc agaatatctg gctaacaatg tagcgtccag ccccttacca
     1441 taaaaggaag taaagagagg tcaagaatta tgattactac ccgaactgcc aggcagtgtg
     1501 gacaagcaga ctacggatgg ttgcaggccc ggtatacttt ttcctttgga cactacttcg
     1561 acccgaaatt gttaggctat gcctccctgc gtgtgcttaa ccaggaagtg ctggccccag
     1621 gtgccgcctt tcagccgcga acctatccca aagtcgatat tttaaatgtg attctggatg
     1681 gagaagcaga gtatcgcgac agcgaaggca atcatgttca ggccagcgcc ggtgaggcgt
     1741 tgctgctctc tacccagccg ggtgtcagct acagcgaaca caatctccgc aaagacaaac
     1801 cgttaacacg aatgcagctt tggctggacg cctgcccgca gcgagagaat ccgctgattc
     1861 aaaaactggc gcttaatatg ggcaagcagc aattaatcgc ctcgccagag ggtgcgatgg
     1921 gaagcctgca attacgccag caagtgtggc tgcaccatat cgtgctcgac aaaggcgaaa
     1981 gtgcgaattt ccagttgcat gggccacgcg cgtatttgca atcgattcac gggaaatttc
     2041 atgcgcttac gcatcatgaa gagaaagcgg cgctgacctg cggtgatggg gcgtttattc
     2101 gtgacgaggc taacattacg ctggttgccg attccccact gcgcgctttg ctgatagatt
     2161 tgcctgtcta gttgttttta cagggagatg atgatgagta aaaaatcggc caaaaagcgc
     2221 cagccagtga agcccgtggt ggcgaaagaa cctgctcgca ccgccaaaaa ttttggctat
     2281 gaagagatgt tgagcgagct ggaagccatc gtcgcggatg ctgaaacgcg tttagccgag
     2341 gatgaagcta ccgcgtaaag acaggttgcc ggatgcggcg aaaacgcctt atccggccta
     2401 caaaatccaa caacattaac gaattaaaca acccgtaggc cggataagat gcgccagcat
     2461 cgcatccggc acgataccca aaacctggcg tttatctggc cttgctcgcc ataatctcga
     2521 taatctgccg atccgtttgc tgcatcgaat ggcttgctaa tgcacacagg ttggcaatcg
     2581 actgctcaac atcatgcgcc acaatccctt cattgccggt cacggcggta tcatccagcg
     2641 ccattaacac cgctttccac gcagccgaag cactggtcga aaccttcatc gcgcagctgt
     2701 tcgacgcacc atcgcaaatc atgccgctga catcgccgat catactgctg atcgccatcg
     2761 agatggtttc ataacgccca tccaccagcc atgccatccc ggcggcggcc cccattgctg
     2821 cggtcgttgc ggcacacagc gcagacaaac gcggtaactg gttatggatg taaattgcgc
     2881 tcaaatgaga aagcatcagc gcacgcgcca gccgttcatc atccgctccg aagtgttctg
     2941 ctaccaccac cacaggcatt gttgcggtga tcccctggtt acccgagccg gagttactca
     3001 tagccggaag cgtagcgccg cccatacgcg catcggatgc cgcgctggta cgaatcacaa
     3061 tggatgaaga gagatctttc gccagcaaac cgcgcgcgca ctgtttttcc agcgtcgcgc
     3121 caatatgcag cccccactta ccgctcaaac cttcctgcga taacgcgcaa tttagcttcg
     3181 cggaatcgag aataaagcgg atcgccgcaa acgggacttc attgacgaac ttcaggatct
     3241 cagccagcgt cgttctggaa agcaccgtca gcggagactc ttgctcgccc tctaccacac
     3301 acgcctgctg ggtaaacacc acaccatcgt gcgtctcaat atgcacaatg ttggtatgcc
     3361 cgccgacgat ggtgacacac gcccacttct caccgttcca gactttggcg cgtgagaaga
     3421 ggatttcatt gcaaggttcc tggatcttaa cggagacttt ccccgccgcc agcagtgctt
     3481 tggcatcggc aattgcctgc gcagttgcgt ctttcagcac ttccagcccg gcgttggcat
     3541 ttccacctaa cgcccccagc gccgccgcaa tcggcagccc caccattccc gtgccgggaa
     3601 cggtgacgcc cagaccgttc ttcatcagat ttggcgaaac ccaggcttct acacgttcaa
     3661 ccggaccttc cagttctgcc gcagcaaccg ccgccgccag cgccagtgaa atcggttcag
     3721 tacatcccag cgccggtttt acttcctcct gaacggcgag gatgtaacgc tgccataacg
     3781 gatttaaagt cgaatcaaac atattaaaaa ccttaaaatt tcaggtaaat caagaaaatg
     3841 ccaggaacgg agaaacacaa agcaacaaac cagtgacgat aatcaggtac agagacatcc
     3901 ctttgtattt gtgcaatgcc ggtactttgt aaaccagcca cgccgggatc aggcacccta
     3961 ccatgccgaa aatcgggcta cagatagagg tgaagctcaa caccggtgcg ttcagtacga
     4021 tggcgctcca ggccagcaaa atggcgaaaa tcatgatgcc gcgctgaaca agattttcgt
     4081 taatcttctc ggcaggcatc ttgcgacgca ggatgttcat tacgatccct tgcgttgctt
     4141 cgcgaaagcc taaatagacg ccaaagaatg cagtcatgac agcaaagata ttgagaatga
     4201 cgctgaccac tttcacccac gctgcgccgt caccgctaat aaactgtgcg gcaatcgcca
     4261 gcgcggaaat attctgctcg taggctttta ccgcttcgtc atgtcccatc gccagcgtga
     4321 acgacacggc gtagaaaaag acggtgacaa acaaaatgcc aaacgcgata ttcattgccc
     4381 gcaatgcttt atgccgcgcc acttcaattg atttttcccg cgagcgataa gagatcacca
     4441 tcgggcttaa cgtctggata aacagaatcg acgtcagggt aaacggcagc gtaataatgg
     4501 cgtttttcac cagcagcccc agcggcggta gcgaaccgac gttatacaga tgccacattc
     4561 ccaccatcga cacgcccagc gccgcgacca ccagcagctt tgtcagcacc atgccagtcg
     4621 aaattttgaa taacaatttc tcgccgcgtg aagagatcgc caccagaatg caaatcagta
     4681 ccagaccata aaagggactg tctgacagca acccttccgt cacgccgaag gtatgcaggt
     4741 aggaagcact atcgttggtg atggcggtgg aatagacgaa catccaaatc accagcatta
     4801 cgaaatagag tgcacctaac aggatgcccc agtttttacc taaataaccg ctaatgacgc
     4861 tcgggtaatc tttacattct ggtgattctg ccagcgtatt aataaacaac cgctgaaaca
     4921 gatacattgc cgggtaacca atcaccgatg agagcaaaaa tacccacaat cccatcaaac
     4981 cgacctgcac cgggagaaaa acaatccccg cgccaatcgc catcccgata ctcataatca
     5041 cccagccggt gtcagtactg tcgaatttga tcgcttctcg ccactcgctc tcactcattc
     5101 cagcacgacc cgccggggtc gaagcgtctg caatgacgcc tttattcgat gcaatttcca
     5161 taatttctcg ctcaatattt tgtagggctt attatttttt tccgagccgc atcaaggcga
     5221 tatgcggtct acgtttttgc aggcgagtta ttagaataaa gaaatgatac gcgcgaggtc
     5281 agagaaaatc ttcacaatca agactttgaa ataacggaga tggataagaa ttttctacta
     5341 aattaatcgc aagagaatat tcattctcta tttgcggcag atcacaaaaa aaaggtgcac
     5401 atttgtgcac ccaaggatga aagctgacag caatgtcagc cgcagaccac tttaatggcc
     5461 agtcctccgc gtgatgtttc gcggtattta tcgttcatat ctttgccggt ttcatacatc
     5521 gtctcgatca ctttatcgag tgaaacacgc ggtgccgagg tgcggcgcat cgccatccgc
     5581 gcggcgttta ctgctttcac ggcattaatg gcattacgtt caatgcacgg gatttgtacc
     5641 tgtccggcaa ccggatcgca ggtcagtcca aggttatgct ccatcgcgat ttccgccgca
     5701 ttgcatacct gcgccggact gccgcccagt agttcagtta accctgccgc cgccattgaa
     5761 caggccacgc caatctcccc ctgacagccg acctccgcgc cagagatgga ggcgttcatt
     5821 ttatacagcg cgccaatagc cccggctgca agaaaatagc gggcaattga ccgctcgttt
     5881 accggacgac ggaacttatc gtaataagcc agcactgccg gaataatgcc gcacgcaccg
     5941 ttagtcggtg ccgttaccac gcgcccgcca gctgcgtttt cttcactaac cgccagcgcg
     6001 tacatgttga tccagtcgat gacattcatc ggatcgttag agatgttatc gctggaaacc
     6061 agttgacgac gcagcgctac ggcacggcgc ggcacattga gcggaccagg cagcacgcct
     6121 tcagtgttca tcccgcgttc aataccgtca tgcatcactt gccagatacg ggcaaaaccg
     6181 gcgtcaattt ccgctttact gcgcagcgct agctcgttgt gcatcatcag gccagaaata
     6241 gacaggccgt tgtaatcaca cattttcagc agttcacctg ctgagtggaa atcgtaaggt
     6301 acggacgttt cgacatcgtg agacaggccg aagtgttctt cttcgacaat aaacccgccg
     6361 ccgacagagt aataggtttt acttaatagc tcttcctgtg ccttccaggc agtgatccgc
     6421 attccgttct catggcgagg caacatttcg ggatggaaga taatgttctt tgctacagga
     6481 aaatcaacaa tatgcgcacc agatgccact ggcagccgcc cgctgcgcgt tactaactct
     6541 ataaatgcag ggatctcatc aatgacaaca tcctgcggac tgtttcctgc cagtcccatg
     6601 atgatggcga catccgtggc atggcctttg cccgtcagtg acaacgaccc gtacagatcg
     6661 accacaatat ggctcgtcgc ggttaataag ccgctacttt ccagccgatc aataaaactt
     6721 tttccggcat tcattggccc cacggtatgc gaactggagg gaccaatccc aattttgaaa
     6781 atatcgaatg cactaatcat atccacaccc tcggattgcc gttcagtgaa gtggagcgga
     6841 acgaccttac gaccgtcccg ctcacgaggc tttacgcact acgtactgcg atagcttcaa
     6901 tttccagctt cacatctttc ggcaaacgcg cgacctgtac acagctccgg gtcgggtagg
     6961 tcgcctgatg ctcatcgaag aactgcttat agacttcgtt gatggtggca aaatcattca
     7021 gatcggtgat gaacacggtc atcttgatga tatcgcccac gctcagcccg gcagcaacca
     7081 cgatcgcttt gacgttttcg aggcttaaac gcgcctgatc ttgcacatca gccgggatct
     7141 caccggtctg tgggcaaacc ggaatttgac cggaggtgaa gaccatgctg cctaaatcaa
     7201 cgccttgaac ataagggccg attgcgcctg gggcacgttg cgtttcgata atctttttca
     7261 tacatcctcc ggcgtcagag cgcctgggta aaggtacgtg aaataacatc ctgctgctgt
     7321 tcacgggtca gtgcgttgaa gcgcacggcg tagccagaga cacggattgt caggttagga
     7381 tatttttccg ggtgctcgat ggcatccagc agcatttccc gattcattac gttgacgttg
     7441 aggtgttgac cgccttcgac atccgcttcg tggtggaaat acccatccag caggccgaca
     7501 aggttggttt tacgtactgg atcttctttg cccagcgccg cagggacgat tgagaaggtg
     7561 tacgagatcc cgtctttagc gtaggtgaac ggcagtttcg ccaccgacgt caatgaggcc
     7621 acggcacctt tgcggtcacg accatgcatc gggttagcgc ccggcgcgaa cggtgttccg
     7681 gcgcgacgac cgtccggcgt attaccggtt ttctggccgt acaccacgtt agaagtgata
     7741 gtcagaatcg actgggtagg gacggcgttg cgataggttg gcagcgcttt aattttcttc
     7801 ataaaacgtt caaccaggtc gcaggcaatg ctgtctacgc gctcgtcgtt gttgccgtac
     7861 tgcggatatt caccgtcgat ttcaaagtcc accgccaggc cgttttcgtc acggattggt
     7921 ttcacgcggg catatttgat cgcagacagg gagtccgttg ccaccgacag gcccgcgatg
     7981 ccgcatgcca tggtgcgata gacatcacga tcgtgcagcg ccatcagcga agcttcgtag
     8041 ctgtacttgt cgtgcatata gtgaatgata ttcagcgcac tgatgtactg caccgccagc
     8101 cagtccatga agtgatcgag gctgtccatc actttgtcgt aatccagcac atcgtccatc
     8161 aacggtgctg ttttcggccc gacctgaatc ttcagcttct cgtccacccc gccgttaatt
     8221 gcgtagagca gcgttttcgc caggttagcg cgtgcaccaa agaactgcat ttgcttacca
     8281 atcaccattg ggctgacgca gcaggcaatc gcgtaatcgt cgctgttgaa gtcagtacgc
     8341 atcagatcgt cgttttcata ttgcaaggaa gaggtgacga tcgacacctg cgcggcatat
     8401 tttttgaagg caatcggtaa ttcttccgac caaagaatgg tcaggttagg ttccggtgca
     8461 ggccccatag tgtgcagggt gtgcaaatag cggaaggagt ttttggtcac cagcgtacga
     8521 ccgtccagcc ccatcccgcc gatcacttcc gtcgcccaga ttgggtcgcc ggagaacagc
     8581 gaatcaaatt ccggtgtacg caggaagcgc accatacgga tcttcatgat gaagtgatcg
     8641 atcagttcct gtgcctgctg ctcattgagt acgccagctt taaagtcgcg ctcaatgtag
     8701 atatccagga acgatgccgt gcgacccagc gacatcgcgc cgccgttctg tgatttcact
     8761 gccgccagat aagcgaagta gagccactgc accgcttcct gcgcattctg cgccgggcga
     8821 gagatatcaa agccatattt cgccgccatt tcctgaatct gcaacagcgc atgacgatgc
     8881 tctgccagct cctcacgcag acggatggtg gcttccagat cctcgccttt ttccagacga
     8941 gactggagat cggcaaattg cagttcgcgt tcacgcacca gataacgaat gccatacagc
     9001 gctacgcggc gatagtcacc gataatgcgc ccacgtccat aaccatctgg taaaccggtc
     9061 agtacgccag atttacggca gcgcagcata tccggtgagt aaacatcaaa tacgccctgg
     9121 ttatgggttt tacgcagatc ggtaaacaga tattcaaatt cactgtccat ttcacggcca
     9181 taggcgtgga atgaactttt aatcatatta atgccaccga acgggtgtag cgcacgtttc
     9241 aacggcgcat ccgtttgcag gccaacaatt ttttccagcg gctggttaat atatcccgca
     9301 tcatgagcgg taattgtggt ggcaatattg gtatcgaaat caaccggcgc gtgggttgcg
     9361 ttttcgatac ggatgccttc catcaccttt tcccacaatt ccgtggtggc aggcgtcgct
     9421 tcggcgagaa aagattcatc accttcatac ggtgtatagt tatgttgaat aaaatcgcgg
     9481 acattaattt cgcttttcca gtccgtacct ttaaagccaa gccatgcgtc ggcgtacagc
     9541 ttatcgctgg tatcaatatc taccttcatg aaaaataatc tctctacaat acttcaacta
     9601 aattatgcaa attctgcggg cgcgttaact ttgcctaaat gaatggcatc caaagcaatc
     9661 attttttctt cgttagtcgg aataacggcg caaatgacac gcgcattttc actggaaaca
     9721 attcgctcgc caaaagagtt agatcgatta ttcatttctg tatcaatctc tacgcctaat
     9781 acagccaaat gttccatgac cagacgacga attaaacttg aattctctcc tattccgccg
     9841 gtgaatataa ttccatccag gcgacgtaat gaagctgcgt gtccggcaat atgacgggca
     9901 attcggtgaa caaaggtttt aattgccagc tgcgcgcgtt cgtgaccttc atgccaggct
     9961 ttttccagaa cacgtaaatc cgaagaaaga ccggaaatac ctaataatcc cgactcttta
    10021 ttcactacgc gttccaggtc acccaggctc tggttggttt ggctggcgac ccaggacatc
    10081 gcaccaaagt cgacatcgcc actgcgggta cccatcatca agccttccag cggcgtcatt
    10141 cccattgagg tatcaacact ctgaccgttg cgaaccgcgc agattgacgc gccattgcca
    10201 agatgcgcca caaccaggcc ggagtcatct tccgccagat tcagcagcga atgtgcgcgc
    10261 tgggaaacat agcggtgcga cgtgccgtgg aaaccataac ggcgtacacc taactcttca
    10321 taatatttcc acggcaggcc gtataaataa gcttccggag ccatcgtctg gtggaaactg
    10381 gtatcaaata ccgccacctg agttacgccc ggaaataatt gctgcgccga ttcaatacca
    10441 cttaaattgg cgtaattatg caggggtgcc agtggagaaa cgcgacggat attatcaatg
    10501 acttcatcgg taataatggc ggactcggta aaaatactgc cgccgtgagc gatgcggtgg
    10561 ccaattaagg ccacgctgtc atttaaattc cgtttttcca gttcaaatgc aattgccttc
    10621 aatgcacctt cgtagctgtg gtgagccagc ggtgctggct ctcccccatt tacggataag
    10681 aatgcatttt ccgagttaat accgtcggca atacctgaca ttaatacttc acagtcgctg
    10741 gcatcgagta cggaaaactt aatcgaagac gaaccacagt taataaccaa aacaaccgga
    10801 aattcattca tctcttttct catcctgagt tacggattaa aacagtttgt atacgatgtt
    10861 caggatggtc agcagaccaa tcacggtaac aaacacgtta tccagacgac cacggtattt
    10921 cgccagagac ggcgctttac ggatggcata catcggcaac aggcacagca gggatgcgat
    10981 aatcggtgcg cccatggctt caatcaggtc gaggatgttc gggttggcgt aggcaacaac
    11041 ccaagtggag cccatgatga agatcatgct gagagtattc agtttaccca gcgacacttt
    11101 ggttttgtca cctttataac cgaacttcag aatcagacca ttcaagcctt ccagcgtccc
    11161 cagatagtga ccgaagaaag atttgaagat agccacgagt gcgatgatgg aagccgcata
    11221 ttccagtgta attgcgaacg ttgttttcgt accggtcatg gacgcaaagt ggttagccag
    11281 ataagaaagc actggaatat tctgcgcttt ggcttccgcc atgttggccg gagacagcgt
    11341 aaacaggcag ctaaaggcaa agaacatcac cactgcaacc atcagcatgc tggcacgaga
    11401 aatgatttgg gaacatttac gttcggtgaa gtcgcgaccg aagtctttct catactcttc
    11461 acgtttagaa accacgaagg aagagacgat tggcgagaag ttaaaggaga aaaccatgat
    11521 ggaaatcccc agccagacag tgatcaggat accgtcatga ccggttaacg acagcgaacc
    11581 gaggtcaacc tggtcgataa ctgcagagtt ccagtaaggg atcagcgaca aagaaatcag
    11641 caccaggctg gcgataaacg gccataccag gtagctcatc actttaacca tcagatcctt
    11701 accaaaccag atgacgaaag ccatcagcag caacaggaac agcgccacaa agccgcgatt
    11761 cagcggtgca aagccgagct ggttttccca gaacgtcata aaggtattgg taatagtaac
    11821 gccataaatc cacagcagtg ggcaaatcgc gaagaagtac aggaacgtga taaccacgcc
    11881 gccagtttta ccaaaatgct cttccaccgt ttccgtaatg ttgccggaag gattagagcc
    11941 agaaagacac agacgcgcca gcgcccggtg gcaataaaac gcgatggggt atgccaatac
    12001 caacatcaga agaatcggga tcagtccgcc aaaacctgcg cggataggga agaacagcac
    12061 cccggcgccg attgccgtac caaacaagcc taacgtccat gtggtatctg atttacgcca
    12121 ggacgattgt tttgtctggc tggatacaat gctatctgaa gtactcatat cctatcctca
    12181 acgaattaat taagcgtcaa cgaaaccggt gatttgagag acgcgagaaa gatcgatatt
    12241 gccgccggaa ataatactga cggtttttct gttttgaata tattggtcta atttaccgct
    12301 taataatgca gcacatgcca gagcgcctgc gccttcggtg acgactttat tgcgctgaat
    12361 taaggcaatc atactgtttc tgatttcgtc ttcgctgacc agcacgatgt catcgactaa
    12421 ttcacgaacg atttcgtaag ttaaattacc cgggcgggcg acatcacaac catccgccag
    12481 ggtgccggta gttcggtgcg tggttatttc tccggagtgg aaagaagccg ccatgccgtg
    12541 aacgttttct gactgtacgc caataacacg aatggtcggg ttaatagatt taattgccac
    12601 cgcaatacca gcaattaaac cgccaccacc aattggcaca atcacgttat cgacatcata
    12661 gagatcttcc ataatttcca ggccaatcgt tccctgacca gcaatcactt tcggatcatc
    12721 gtaaggtggg ataaaaatac ggccttccat ttcgacaatt tcgctcactt tagcgatagt
    12781 gtcgttgaag ttatcaccat gcagaacgac ttctgcggag tagtcgcacg ttgccgctac
    12841 tttggatttt ggcgcacctt ttggcatcac cactttaccg tcgataccca gcatcgcgca
    12901 ggagagggaa accccttgcg catggttgcc cgcagaacag gccaccacgc ctttgcgttt
    12961 ttccgcatcg gtcagtgaac ttaatttatt aaatgcgcca cgaattttaa atgaacccgt
    13021 acgctgcata ttttcaaact tcaggaatat ttcacctttg caacgttcac taaaatagtt
    13081 ggagcgaggc atacctgttt tataaattcg cccagccagt cgttgtttcg cttcaataat
    13141 gtcatcaata gcaaccggca gatcgtatgt aatatgcatt ataaaacctc ttcgcctgaa
    13201 ttaaatatgt aggtaaccgt aaccgacacc tgcaagacag acaggtggat tatttatgtt
    13261 taaataaaaa caaataacta accaacttct attaattgcc ttcgtctaca cccattataa
    13321 gatgaatact ctttggctaa ttccaccaaa accgatgctg cttttttaat acgataattt
    13381 ttcgaccata ccgcggcata ttgtgccaca ggtaatgttt cttcaaccgg aatagtaata
    13441 aattgattag aaccaaaagg tgacgtcata tcacaaggaa ttacagttaa gaaatcagca
    13501 ttgagaacaa gattataaat tgtcacgact gagtcggttt taacgatgtt ttcaatactg
    13561 atgccatttc tttgtaacgt agtaagcagt tcgctgtagt accccatatt ggtttgtggc
    13621 aacacccact gttcgttctt caacgactcc agcgtggtgg tgccggtgca tgttcgggac
    13681 ttactggcta ccagcacaaa ctcggactcg aacagcggct caacatgtaa atcctgaagc
    13741 ttcatttctg cacttaacgt accaatcgca aaatccagtc gaccgtcgcg gattgccggt
    13801 aagaacgaag acagttgcgc ttcatacata gaaacctgcg cttttgggaa cacctctttg
    13861 aacttattga tcatccctga cataaaagta aaaccaatca atgaaggaaa accaaatgag
    13921 acttccacca ccgcctcaga agacataccg cttatctcat taaccatatt tttcatttca
    13981 cgggtaatgg attcggaacg ggagagtaac aattgaccgg caggtgttaa tgttacaccg
    14041 gtatttttcc gcaccactaa ttccacacca aaataatctt caatatcgtt aatgatttta
    14101 ctgacggctg gttgagttaa ccctaattct tttgcagccg agccgataga gccacttcta
    14161 atgacttcct gaaagactac caggtgctgc gttttcggaa gaagaatagt gctcataacg
    14221 acctacgtta attacctcat tgacggcatg aagtgtatca aaatgaaatg aacaggatat
    14281 gtgcgaccac tcacaaatta actttcaata ctttccaggg tatcgtcatg tcaattaaat
    14341 acctttaaat atcaataagt taaagtataa aaatcagcat aaacc
//
LOCUS       DAZFKF010000114        14163 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFKF010000114 DAZFKF010000000
VERSION     DAZFKF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.116"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            190..1248
                     /gene="citC"
                     /locus_tag="ACHKA1_004297"
     CDS             190..1248
                     /gene="citC"
                     /locus_tag="ACHKA1_004297"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO5681865.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTKG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1263..1559
                     /gene="citD"
                     /locus_tag="ACHKA1_004298"
     CDS             1263..1559
                     /gene="citD"
                     /locus_tag="ACHKA1_004298"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO5681866.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1556..2464
                     /gene="citE"
                     /locus_tag="ACHKA1_004299"
     CDS             1556..2464
                     /gene="citE"
                     /locus_tag="ACHKA1_004299"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO5681867.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2475..4007
                     /gene="citF"
                     /locus_tag="ACHKA1_004300"
     CDS             2475..4007
                     /gene="citF"
                     /locus_tag="ACHKA1_004300"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO5681868.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4011..4562
                     /gene="citX"
                     /locus_tag="ACHKA1_004301"
     CDS             4011..4562
                     /gene="citX"
                     /locus_tag="ACHKA1_004301"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO5681869.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALCALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCAQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4537..5415
                     /gene="citG"
                     /locus_tag="ACHKA1_004302"
     CDS             4537..5415
                     /gene="citG"
                     /locus_tag="ACHKA1_004302"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO5681870.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5466..6929
                     /gene="citT"
                     /locus_tag="ACHKA1_004303"
     CDS             5466..6929
                     /gene="citT"
                     /locus_tag="ACHKA1_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO5681871.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7043..7849
                     /gene="rna"
                     /locus_tag="ACHKA1_004304"
     CDS             7043..7849
                     /gene="rna"
                     /locus_tag="ACHKA1_004304"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO5681872.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEMQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8079..8489
                     /gene="rnk"
                     /locus_tag="ACHKA1_004305"
     CDS             8079..8489
                     /gene="rnk"
                     /locus_tag="ACHKA1_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO5681873.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8627..8716
                     /gene="yldA"
                     /locus_tag="ACHKA1_004306"
     CDS             8627..8716
                     /gene="yldA"
                     /locus_tag="ACHKA1_004306"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO5681874.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8720..9958)
                     /gene="ybdR"
                     /locus_tag="ACHKA1_004307"
     CDS             complement(8720..9958)
                     /gene="ybdR"
                     /locus_tag="ACHKA1_004307"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFO5681875.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10178..10606
                     /gene="uspG"
                     /locus_tag="ACHKA1_004308"
     CDS             10178..10606
                     /gene="uspG"
                     /locus_tag="ACHKA1_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO5681876.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10727..12292)
                     /gene="ahpF"
                     /locus_tag="ACHKA1_004309"
     CDS             complement(10727..12292)
                     /gene="ahpF"
                     /locus_tag="ACHKA1_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO5681877.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12421..12984)
                     /gene="ahpC"
                     /locus_tag="ACHKA1_004310"
     CDS             complement(12421..12984)
                     /gene="ahpC"
                     /locus_tag="ACHKA1_004310"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO5681878.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13356..14102
                     /gene="dsbG"
                     /locus_tag="ACHKA1_004311"
     CDS             13356..14102
                     /gene="dsbG"
                     /locus_tag="ACHKA1_004311"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO5681879.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLKVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3360 a         3759 c         3566 g         3478 t
ORIGIN      
        1 taactctatg tgaaataaat caaaatttca cgccaaaata ctccttagga tgtatagcga
       61 aaggagaaaa agatatacct cgatcacccc cctttctccc aagtgaaaat aaaaggttat
      121 cagtttgcaa cattgaacaa cattcgttgc aaatcgataa caacatgcac cttcaggata
      181 ctatttatta tgttcggcaa tgatattttc acccgcgtaa aacgttcaga aaataaaaaa
      241 atggcggaaa tcgcccaatt cctgcatgaa aatgatttga gcgttgacac cacagtcgaa
      301 gtatttatta ccgtaacccg cgatgaaaag cttatcgcgt gcggtggaat tgccggaaat
      361 attattaaat gcgttgctat cagtgaatcc gttcgcggtg aaggactggc gctgacatta
      421 gccactgaac tgataaacct cgcctatgag cggcacagca cgcatctgtt tatttatacc
      481 aaaaccgaat acgaggcgct gttccgccag tgcggttttt ccacgttgac cagcgtacca
      541 ggcgtgatgg tgctgatgga aaacagcgcc acgcgattga aacgctatgc cgaatcgctg
      601 aaaaaatttc gtcatcccgg gaacaagatt ggctgcattg tgatgaacgc caatcccttt
      661 acgaagggtc accgttatct gattcaacag gctgcggcac agtgcgactg gttgcatctg
      721 tttttagtca aagaagattc ttcacgcttc ccctatgaag accgactgga tctggtgtta
      781 aaaggcaccg ccgatattcc acgcctgact gtgcatcgcg gctccgaata catcatctcc
      841 cgcgctacgt tcccttgcta cttcattaaa gaacagagcg tcattaacca ttgttacacc
      901 gaaattgatt tgaagatttt ccgtcagtac ctcgctcccg cgctgggtgt aactcaccgc
      961 tttgtcggta ctgaaccctt ttgtcgcgtt accgcccagt acaaccagga tatgcgctac
     1021 tggctggaaa cgccgactat ctccgcaccg cccatcgaac tggttgaaat tgagcggctg
     1081 cgttaccagg agatgccgat atccgcttcc cgggtacgtc aactgctggc gaaaaacgat
     1141 ctcacggcta tcgcgccgct ggtccctgca gtcacgctgc attatttgca gaacctgctt
     1201 gagcactccc gccaggacgc ggcagctcgt caaaagaccc ccgcatgaga aacaggtgaa
     1261 aaatgaaaat aaaccagccc gccgttgcag gcacccttga gtctggggat gtgatgatac
     1321 gcatcgcccc actcgatacg caggatatcg acctgcaaat caatagcagc gttgagaaac
     1381 agtttggcga tgcaattcgc accaccattc tggacgttct cgcccgctac aacgtgcgcg
     1441 gcgtacagct gaatgtcgat gacaaaggcg cactggactg cattttacgt gcacgactgg
     1501 aagccctgct ggcacgcgcc agcggtatcc cggctctgcc atgggaggat tgccaatgat
     1561 ttccgcttcg ctgcaacaac gtaaaactcg cacccgccgc agcatgttgt ttgtgcctgg
     1621 tgccaatgcc gcgatggtca gcaactcctt catctacccg gctgatgccc tgatgtttga
     1681 cctcgaagac tccgtagcat tgcgtgaaaa agacaccgcc cgccgcatgg tttaccacgc
     1741 gctgcaacat ccgctgtatc gcgatattga aaccattgtg cgtgtcaacg cgctggattc
     1801 cgaatggggt gtcaacgacc tggaagccgt cgttcgcggt ggtgcggacg ttgtgcgtct
     1861 gccgaaaacc gataccgctc aggatgttct ggatatcgaa aaagagatcc tgcgtatcga
     1921 aaaagcctgt ggtcgtgaac ccggcagcac cggcctgctg gcggcgattg aatctccgct
     1981 ggggattacc cgcgcagtgg aaatcgctca cgcttccgag cgtttgatcg gtatcgccct
     2041 cggtgcagaa gactatgtgc gcaacctgcg tacagaacgc tccccggaag gaactgaact
     2101 gctgttcgca cgctgttcca ttttgcaggc cgcgcgctct gcgggtattc aggcgttcga
     2161 taccgtctat tccgacgcta acaacgaagc cggattcctg caagaagccg cccacatcaa
     2221 gcagctgggc tttgacggca aatcgctgat caacccgcgt cagattgatc tgctgcacaa
     2281 cctctacgca ccgacccaga aagaagtgga tcacgcccgc cgcgtcgtag aagccgctga
     2341 agccgccgct cgcgaaggcc tcggcgtggt ttccctgaac ggcaagatgg tggacggtcc
     2401 ggttatcgat cgcgcccgtc tggtgctctc ccgtgcagaa ctttccggca tccgcgaaga
     2461 ataaggcaat caaaatgacg cagaaaattg aacaatctca acgacaagaa cgggtagcgg
     2521 cctggaatcg tcgcgctgaa tgcgatcttg ccgctttcca gaactcgcca aagcaaacct
     2581 accaggctga aaaagcgcgc gatcgcaaac tgtgcgccaa cctggaagaa gcgattcgtc
     2641 gatcaggttt acaggacggc atgacggttt ccttccatca cgctttccgt ggcggtgacc
     2701 tgaccgtcaa tatggtgatg gacgtcatcg cgaagatggg ctttaaaaac ctgaccctgg
     2761 cgtccagctc cctgagtgat tgccatgcgc cgctggtaga acacattcgc cagggcgtgg
     2821 ttacccgcat ttatacctcc ggcctgcgcg gtccactggc agaagaaatt tcccgtggtc
     2881 tgcttgcgga accggtgcaa atccactctc acggcggtcg cgtgcatctg gtacagagtg
     2941 gcgaactgaa tatcgacgtg gctttcctcg gcgtcccgtc ctgcgatgaa ttcggtaatg
     3001 ccaacggcta caccggtaaa gcctgctgcg gctccctcgg ctatgcaatg gtcgatgccg
     3061 acaacgcaaa acaggtcgtg atgctcaccg aagaactgct gccttatccg cataatccgg
     3121 caagcattga acaagatcag gttgatctga tcgtcaaagt tgatcgcgtt ggcgatgctg
     3181 caaaaatcgg cgctggtgcg acccgtatga ccactaaccc gcgcgaactg cttattgccc
     3241 gtagcgctgc ggatgtgatt gtcaactctg gctacttcaa agaaggtttc tccatgcaaa
     3301 ccggcaccgg cggcgcatcg ctggcggtaa cccgtttcct ggaagacaaa atgcgtagcc
     3361 gcgatattcg cgccgacttc gcccttggcg gtattaccgc gacgatggtt gacctgcacg
     3421 aaaaaggtct gatccgcaaa ctgctggatg tgcagagctt tgacagccat gctgcgcagt
     3481 cgctggcccg taaccccaat cacatcgaaa tcagcgccaa ccagtacgct aactggggtt
     3541 cgaaaggcgc atcggttgat cgtctcgacg tggtggtact gagcgcgctg gaaattgaca
     3601 cccagttcaa cgttaacgtg ctgaccggct ctgacggcgt actgcgtggt gcttccggtg
     3661 gtcactgcga taccgcaatt gcctctgcgc tttccatcat cgtcgcgccg ctggtacgcg
     3721 gtcgtattcc gactctggtg gataacgtgc tgacctgcat caccccgggc tccagtgtcg
     3781 atattctggt cacagaccac ggtatcgcag ttaacccggc acgtccggaa ctggcagaac
     3841 gtctgcagga agcgggcatt aaagtggttt ccattgagtg gctgcgcgaa cgcgcgcgtc
     3901 tgctgaccgg tgaaccacag ccgattgaat tcaccgaccg cgtcgttgcc gttgtgcgtt
     3961 accgcgatgg ctcggtgatc gatgttgtgc atcaggtgaa ggaataagcc atgcacctgc
     4021 ttcctgaact cgccagccac catgcggtgt caattcccga gctgctcgtc agccgggatg
     4081 aaaggcaagc acggcaacac gcctggctca agcgccatcc tgttccactg gtctccttta
     4141 ccgtggttgc gcctgggccg attaaagaca gcgaggtcac acgccgaatt tttaatcatg
     4201 gcgtgaccgc cttgtgtgcc ttagccgcaa aacagggctg gcaaattcag gagcaggctg
     4261 cactggtttc cgccagcggg ccggagggca tgttgagcat tgccgccccg gctcgcgacc
     4321 tcaagctcgc caccattgag cttgaacata gtcatcctct cgggcggtta tgggatatcg
     4381 atgtcctgac gcccgaaggc gaaattctct cccgccgcga ctattcactg ccgcctcgcc
     4441 gctgcctgtt gtgcgcgcaa agcgcagccg tctgcgcgcg tggaaaaacc catcaactga
     4501 ccgatttact caaccgcatg gaggcactgc tgaacgatgt cgatgcctgc aacgtcaact
     4561 aaaaccacaa agcttgcgac gtcattaatc gatgagtacg ccctgctggg ctggcgcgcc
     4621 atgctgactg aagtcaatct gtcaccgaaa ccaggcctcg tggatcgcat taactgcggt
     4681 gcgcataaag atatggcgct ggaagatttc caccgcagcg cgctggcgat tcagggctgg
     4741 ttaccccgtt tcattgaatt tggtgcctgt agtgcagaaa tggcaccgga agcggtactc
     4801 cacggattac gcccaattgg tatggcttgc gaaggtgata tgttccgcgc cactgcgggc
     4861 gtaaacacgc ataaaggcag cattttttct ttagggctgc tatgtgcggc aattggacgt
     4921 ttgcttcagc tcaaccagcc ggttacgcca acaaccgttt gttctacggc ggcaagtttc
     4981 tgccgtggcc tgaccgatcg cgaactgcgt accaataatt cacaactgac ggcaggtcaa
     5041 cggttgtacc agcagcttgg ccttaccggc gcacgcggtg aagccgaagc gggttatcca
     5101 ctggtgatca accacgcctt gccgcattac ctcactctgc tggatcaggg gttagatcct
     5161 gaactggcat tgctcgatac cttgctccta ctgatggcga tcaacggcga taccaacgtt
     5221 gcgtcgcgcg gtggcgaggg gggcctgcgc tggctacagc gcgaggcgca aacattattg
     5281 caaaaagggg gcattcgaac ccccgccgat ctcgattatc tccggcagtt cgacagggag
     5341 tgtatcgaac gaaatctcag tccaggcggc agtgccgacc tgctgatcct tacctggttt
     5401 ttagcacaga tttaattatt taagcacttg ataaattttg aaatattaat tttcggagaa
     5461 cccgtatgtc tttagcaaaa gataatatat ggaaactatt ggccccactg gtggtgatgg
     5521 gcgtcatgtt tcttatccct gtccccgacg gtatgccgcc gcaggcatgg cattacttcg
     5581 ctgtgtttgt ggcaatgatt gtcggcatga tcctcgagcc aattccggca acagcgatca
     5641 gttttattgc ggttactatt tgcgttattg gcagtaatta cctgctcttt gatgccaaag
     5701 aattagctga cccagcgttt aatgcgcaaa aacaggcgct gaaatggggt ctggctggtt
     5761 tttccagcac cacggtatgg ctggtatttg gcgcatttat ttttgcatta gggtatgaag
     5821 tttccgggtt aggtcgtcgc attgcccttt tcctggtgaa attcatgggc aaacgcacgc
     5881 tgacgttggg ttatgcgatt gtcattatcg acattctgct ggcaccgttt acaccgtcca
     5941 acaccgcgcg taccgggggt acggtttttc cggtcattaa aaacctgccg ccgctgttta
     6001 aatcattccc gaacgatccg tccgcgcgtc gtattggcgg ctatttgatg tggatgatgg
     6061 tcattagtac cagtctgagt tcgtccatgt ttgtcaccgg tgcggcacca aacgtgctgg
     6121 gtctggagtt cgtcagcaaa attgccggta tccagattag ctggttgcag tggttcctct
     6181 gcttcctgcc ggttggggtt atcttgctta tcattgcgcc gtggctttcc tacgtgctgt
     6241 acaaaccgga aatcacacac agtgaagaag tggcaacctg ggcgggtgat gaactgaaaa
     6301 ccatgggtgc gctgacacgc agagagtgga cactgattgg ccttgtattg ctcagcttag
     6361 gtttgtgggt atttggcagt gaagtcatta atgctactgc ggtaggtctg ctggcagttt
     6421 cgctaatgct ggccctgcac gttgtgccgt ggaaagacat tacccgctat aacagcgcat
     6481 ggaacacgct ggtcaacctg gcaactctgg ttgtgatggc taacggcctg actcgttctg
     6541 gctttattga ctggttcgcc ggtaccatga gtacgcacct ggaaggattc tcaccaaacg
     6601 caacggtgat tgtactggtt ctggtgttct actttgcaca ctacctgttt gccagcctgt
     6661 ctgcgcacac cgcaaccatg ctgccggtta ttctggccgt cggtaaaggt attccgggcg
     6721 taccaatgga acaactgtgt atcctgctgg tgctgtctat tggtatcatg ggctgtctga
     6781 cgccgtatgc aaccggtcct ggggtgatta tttacggctg tggctatgtg aaatcaaaag
     6841 attactggcg tcttggcgca atcttcgggg tgatttacat ctctatgttg ctgttggttg
     6901 gctggccgat tctcgccatg tggaactaat acgttcgcca aacgctgaat aaatcatcac
     6961 gcccgccagg taagcccacc tggcgggctt ttttatgatt taatagatag tattcagtgt
     7021 atgtatgagt tccacaccca ttatgaaagc attctggcgt aacgccgcgt tgctcgcggt
     7081 ttctctgctt cccttctctt ctgccaacgc cgtagcgttg caggcaaaac agtatggcga
     7141 ttttgatcgc tatgtcctgg ccctctcctg gcaaaccgga ttttgccaga gtcaacacga
     7201 tcgaaatcgt aacgaacgag atgaatgtcg cctgcaaacc gaaacgacca acaaagctga
     7261 ttttctgacc gtacatggtc tgtggccagg attgcctaaa tcggtcgctg cccgtggtgt
     7321 tgatgaacgc cgctggatgc gcttcggttg cgctactcgc ccaatcccga atctaccaga
     7381 agcgcgcgcc agccgaatgt gttcatcgcc ggaaaccggg ttatcactgg aaacggccgc
     7441 taaactaagt gaagtcatgc caggagctgg cggacgttcc tgcctggaac gctacgaata
     7501 tgccaaacac ggtgcctgct ttggttttga tccggacgca tacttcggta cgatggtacg
     7561 cctgaatcaa gaaattaaag agagcgaagc cggaaaattc cttgcggata attacggtaa
     7621 aacagtgagc cgccgtgact ttgacgccgc ctttgccaaa agctggggaa aagagaacgt
     7681 gaaagcagtt aagctaacgt gccagggtaa ccctgcgtat ttgactgaaa tgcagatctc
     7741 gatcaaagct gacgccatca acgctccgct ttctgccaac tcattcttgc cacaaccaca
     7801 cccaggcaac tgtggcaaaa cctttgtgat tgataaagcg ggttattaat cctctgattt
     7861 acaaaacacg ctaattcgtc ttccataaac caaggcgatt aagatttata cataatgcgc
     7921 tgcattgagg aattccctga atgtgacgca aatcacttca aagagtgagc aacagtcagt
     7981 atcatcatgt gagttaaacc ctcgccgcct gacggtgagg gttttctttt gggtatgttt
     8041 atctgcgaca ctcgcagtac cgacgacatg gagtaaaaat gtccagacca actatcatca
     8101 ttaacgacct ggatgccgaa cgcatcgata ttctgctgga gcaacccgcc tatgctggtt
     8161 tgccaatcgc cgacgcgtta aacgcagagt tggatcgcgc ccaaatgtgt tcgccagaag
     8221 agatgccaca cgacgtggtg acaatgaaca gccgggttaa attccgcaat cttagcgatg
     8281 gcgaagtgcg tgtgcgcacg ctggtgtatc cggcaaaaat gaccgatagc aatactcagc
     8341 tttccgttat ggctccggta ggtgccgcac tgctggggct gcgcgttggc gattccattc
     8401 actgggaact tccgggcggc gttgcaaccc accttgaagt gctggaactc gaataccagc
     8461 cagaagctgc tggcgactac ctgctttaat cccatctgca caagcgataa ctcgctttgc
     8521 ccagaggatg cactcgcatc ctctttacgc aaaatcagca atttttcctg gttctcactg
     8581 ttcacttttg ctgcaaaatt attccgtttc gtttgtggga ataaccatgg cagaagcatt
     8641 ttacatcctg ataggatttc tgattatggc ggcgattatc gtcatggctg tcctttacct
     8701 cgaaaaccac tcctgacgat catattgttc cccccggcat cgcattcacc agacctgaaa
     8761 ccgccttctg cgccgcctct gcgctttgtg caccgggtac cagaatcacc ttacggcact
     8821 cctcttcacg tttttcgaaa atctcatatc cccgggcggc ctcttcaaac ggcatatagt
     8881 gggtaacaat ttcttctggt ttcagtaatc ctttctcaat taacggtaat aattctccca
     8941 gccatgcgtg aacgtgggtc tgtcccattt taaacgtcaa ccctttatca aaggcgtcgc
     9001 caaacaggaa accgtgaata aatccagcgt agacgcccgg tacgctaaca atgccgccac
     9061 gcctgaccgc cgcaatacac tggcgcaacg ctttaccgct gctgccctcc agtttcaggt
     9121 tagtcagcac cgtttccgtg gtgctgcctt tcgcttcaaa accgacggcg tctattactg
     9181 catccacgcc ccggtgacct gccgtttgtt caataattga ctgtgccgga tcgctatctt
     9241 catcgaaatt aatcgggatc gcgccgtagc ggtcagcggc gaaacgcaag cggtagggat
     9301 gatgatcaac aacaaaaatc tgttccgcac cgagcaaccg tgcacaggcg attgtcaaca
     9361 atcccacagg accggcacca tagactgcaa cgcttgaacc ttgttggatc tgcgcatttt
     9421 ttgctgcctg ccatgccgtt ggcagaatat cagaaaggaa aagcgcttta tcatctgaaa
     9481 gcaaaggcgg tactttaaac ggccccacat tccctttagg gacgcggaca tattccgcct
     9541 gcccgccagg aacgccacca tacaggtgac tataaccaaa caatgccgcg ggagcaggta
     9601 tctgtttttt attgagcgca gcgcctttac ccgcattggt attttcgcag gcggcatatt
     9661 gctgcattcg acagaaaaaa cagtcgccac aagcaatgac gaacggaatt accacccggt
     9721 cgcctttttg caaatttttt acgtccttcc cggtttcaac tacttccccc ataaattcat
     9781 gaccaaaaat atcgccatgt ttaacctggg gtattttgcc tcgataaaga tggaggtcag
     9841 agccacagat cgccgttgcc gtaatacgca gaataatatc atctgcctgt tcaatgcccg
     9901 gatcgggaac attttctacc tgaacgtgat gtgggccgtg ataagtcaat gctttcatgc
     9961 aacctccaga gacagttcaa aaaaagtcag tctttaaggg tagctatcac agcgggaaaa
    10021 cgggcggtac cgcaggtttt gggagaattc ctggtgttca gtgagaatat attaatcaca
    10081 gaaaccgttc gtaaggaaaa agaaccagac aaatatcagg gataaatatt ataatcatgg
    10141 cgaatgattt gttcatgatt aacagggaga aagggttatg tataagacaa tcattatgcc
    10201 agttgatgta tttgaaatgg aattgagcga caaagctgtt cgccacgctg aattcctcgc
    10261 ccaggatgac ggagttattc atctacttca cgtactaccc gggtcagcca gcctgagcct
    10321 gcaccgtttt gccgctgatg tgcgtcgttt tgaagagcat ctgcaacatg aagcagaaga
    10381 acgtctgcaa acgatggtca gccacttcac catcgatcct tcccgcatta aacaacatgt
    10441 ccgttttggt agcgtgcggg atgaagtcaa tgagttggca gaagaactgg gggctgatgt
    10501 tgtagttatc ggttctcgca acccatcgat ttcgacccat ctgttaggtt ctaacgcctc
    10561 gagcgtgatc cgccacgcca atctgcctgt actggtcgtt cgctaacaga taaaaatatt
    10621 tatgccgccc tgcctggaac atcccggcag ggctttttaa tgccataaaa aagccgccag
    10681 gtttgacccc ggcggctaag caattgcagg tgaatcttac ttcttcttat gcagttttgg
    10741 tgcgaatcag gtagtcaaaa gcactcagag aggctttggc accttcgcca gtggcgatga
    10801 tgatctgctt gtacggaacc gtcgtacagt cacccgctgc gaacacgcct ttgacgttgg
    10861 tttcgcattt cgcatcaatg ataatctcgc ccatgcggtt acgttcgact gcgccttcca
    10921 gccagttggt gttcggcagc agaccaatct ggacaaaaat accggccagt tcgatgttgt
    10981 gaatatcgcc gctgacacga tctcgatatt ccagaccaac gactttgctg ccgtcgcctt
    11041 tcacttccgt ggtttgcgca ttcagaataa tgtcgacgtt tttcaggctg cgcagtttgt
    11101 cctgcagaac ctggtcggct ttcatttctg gcgcaaattc cagcagcgtt acgtgctcaa
    11161 cgatacccgc caggtcgatt gccgcttcca cgccggagtt accgccgccg ataaccgcta
    11221 cgcgtttgcc tttaaacagc gggccgtcgc agtgcgggca gtaggtcacg cctttagtgc
    11281 gatactgatc ttcgcccgga acgttcatgt tgcgccattt tgcaccggtc gccacgataa
    11341 tgctgcgtgc tttcagtacc gcgccagaag ctgtttcaat ctggtgtaga ccaccttcaa
    11401 ctgctgccgg gatcagtttg ctggcgctct ggctgtcgat cacatcaacg tcgtattcat
    11461 caacgtgaac tttcagcgca cctgccagtt tctggccttc ggtcttcggt acagaaatgt
    11521 agttttcgat atcaacggta tcgaggatct gaccaccaaa acgttcgccc atcagaccgg
    11581 tacggatgcc tttacgtgcg gagtaaattg ctgccgctgc acccgccggg ccggaaccaa
    11641 cgattaatac gtcataagca tcacgcttgt tcagctcttc tgccgcacgt ttttccgcgc
    11701 cagtatcaat tttggcaacg atttcagtca acgtcatgcg gccctgacca aactctttcc
    11761 cgtttacgaa cactgccgga acgcccatca cgttgcgatc ggtgatttcg ttctggaagg
    11821 tgccgccgtc aattgcagtg tgcttgatgc gcgggttcaa tacgctcatc aggttcagcg
    11881 cctgcaccac gtccgggcag ttatggcaag agagcgagta ataggtttcg aattcaaaat
    11941 caccgtcaat atggcgaatc tgctccagca gagactgcgc ttctttcgac ggatgaccac
    12001 cggtccacag taacgccagt accagcgagg tgaactcgtg gcccagcggg gagcctgcaa
    12061 aacgtggtcc ctggttagaa cctgggttgg tgatcaggaa agacggctta cgcaccggca
    12121 agctgttatc ttctttaaag gtgactttgt ctgacagttc tgcgatttca gccaacagtt
    12181 ccttgatttc tgccgattta gcgctgtcat ccagcgtggc aattaactca acaggcttgg
    12241 tcaatttctc aaggtaagcc ttgagttgag ttttcatatt tgtgtcgagc atgtttatct
    12301 cctgggcttt aaacatcatc atgcaagcgg cctctgcggc tacctggatg cagcttgcat
    12361 catggtgcgg ggaagaaaat cgggtgccgc agcacccgat taattacgcg attgcggaac
    12421 ttagatttta ccaaccaggt ccagagacgg agccagagtt gcttcacctt ctttccattt
    12481 agccgggcaa acttcacctg ggtgagaagc tacgtactgt gctgctttga ttttacgcag
    12541 caggtcagac gcgtcacggc caatgccttc agcggtaact tcgattgcct ggatgatacc
    12601 ctgcgggtca acaacgaagg tcgcacggtc agccagacct tcatcttcac gcatgttgtc
    12661 gaagttacgg gtcagggcgc cagtcgggtc gccgatcatc gcatatttga ttttagcgat
    12721 ggtttcagag ctgctgtgcc atgctttgtg ggtgaagtga gtatcggtag atactgcgta
    12781 tacgtctacg cccagtttct gcagttcttc gtagtggtca gcaacgtcac ccagttcggt
    12841 cgggcatacg aaagtaaagt cagccgggta gaagaagaag acgctccagc ggccttcggt
    12901 atctttttcg gtgatttcga tgaattcgcc gtttttgaat gcctggtttt taaaaggttt
    12961 aattttggtg ttaatcaagg acatctatac ttcctccgtg ttttcgatga gatgtaaggt
    13021 aaccaatttt taccgattcg gctaatgcgt ttccattatc aaatcgataa gttttacctt
    13081 acaaccttcg taagacaact ttgtgaactt tgcaccgcca gcggcgataa aaagtaaaat
    13141 ggcccccttt ccaggtgacc gtgtaatctg aactaccctt tggtatcttc agcgcctgtc
    13201 tgcaggctgc ggtgttgctc ttacaaatgc aacaacgaca tggattacaa caccctcata
    13261 aacaaagggc aatcacctga tctaagctct tacctatgac aatgataggt tatgcctttt
    13321 actcgacttt tgcactgact gaaaaggaca aattaatgtt aaaaaagata cttttactgg
    13381 ctctgcttcc tgcaatcgcc ttcgcagagg aacttcctgc tccagtaaaa gcgattgaaa
    13441 aacagggtat tacgatcatc aaaacgttcg atgcccccgg aggaatgaaa ggttatctcg
    13501 gaaagtatca ggatatgggc gtcaccatct acctgactcc agatggtaag cacgctatct
    13561 ctggttacat gtacaacgag aaaggtgaaa acctgagtaa cacacttatc gaaaaagaaa
    13621 tttacgcacc agccggacgc gaaatgtggc aacggatgga acaatcccac tggctcctcg
    13681 acggtaaaaa agacgcgccg gtcattgtct acgtcttcgc cgatccgttc tgcccatatt
    13741 gtaaacagtt ctggcagcag gcgcgcccgt gggtagattc tggcaaagta cagttgagaa
    13801 cattgttggt tggggttatc aaaccagaaa gtccggcgac agccgcggca attcttgcct
    13861 ccaaagatcc cgcaaaaacc tggcaagaat atgaagcctc tggtggcaag cttaagctaa
    13921 aggtgcctgc aaacgtaagt acagagcaaa tgaaagtgtt aagcgacaac gagaaactca
    13981 tggacgatct gggggcaaat gtcactccgg ctatctatta catgagtaag gaaaatacgc
    14041 tgcaacaggc cgtggggttg cccgatcaga aaacgcttaa tatcattatg ggaaataaat
    14101 aaaaggtaat tcatggatgg ttgaactata tccctgcttt tcaaccatcc taaaaaagca
    14161 tga
//
LOCUS       DAZFKF010000115        14044 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFKF010000115 DAZFKF010000000
VERSION     DAZFKF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.117"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..78)
                     /gene="ykgR"
                     /locus_tag="ACHKA1_004312"
     CDS             complement(<1..78)
                     /gene="ykgR"
                     /locus_tag="ACHKA1_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO5681880.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYA"
     gene            complement(552..1694)
                     /locus_tag="ACHKA1_004313"
     CDS             complement(552..1694)
                     /locus_tag="ACHKA1_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFO5681881.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            complement(1929..2849)
                     /locus_tag="ACHKA1_004314"
     CDS             complement(1929..2849)
                     /locus_tag="ACHKA1_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5681882.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGYAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
     gene            3006..3932
                     /locus_tag="ACHKA1_004315"
     CDS             3006..3932
                     /locus_tag="ACHKA1_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5681883.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            4133..5026
                     /locus_tag="ACHKA1_004316"
     CDS             4133..5026
                     /locus_tag="ACHKA1_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5681884.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQQNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLRKYPEISVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINIRLPTAGGLYHWEFEREGKALRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYITSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(5057..6046)
                     /locus_tag="ACHKA1_004317"
     CDS             complement(5057..6046)
                     /locus_tag="ACHKA1_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO5681885.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGSIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(6073..6924)
                     /locus_tag="ACHKA1_004318"
     CDS             complement(6073..6924)
                     /locus_tag="ACHKA1_004318"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO5681886.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINTGYRLID
                     TAASYQNETQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLHAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            7751..8308
                     /locus_tag="ACHKA1_004319"
                     /pseudo
     CDS             7751..8308
                     /locus_tag="ACHKA1_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635811.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8448..9298)
                     /locus_tag="ACHKA1_004320"
                     /pseudo
     CDS             complement(8448..9298)
                     /locus_tag="ACHKA1_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            9556..10425
                     /locus_tag="ACHKA1_004321"
     CDS             9556..10425
                     /locus_tag="ACHKA1_004321"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO5681887.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLFHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(10585..11178)
                     /gene="rclC"
                     /locus_tag="ACHKA1_004322"
     CDS             complement(10585..11178)
                     /gene="rclC"
                     /locus_tag="ACHKA1_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO5681888.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(11190..11426)
                     /gene="rclB"
                     /locus_tag="ACHKA1_004323"
     CDS             complement(11190..11426)
                     /gene="rclB"
                     /locus_tag="ACHKA1_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO5681889.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(11535..12860)
                     /gene="rclA"
                     /locus_tag="ACHKA1_004324"
     CDS             complement(11535..12860)
                     /gene="rclA"
                     /locus_tag="ACHKA1_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO5681890.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVINGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGVAVNERGGIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLIK"
     gene            13086..13940
                     /gene="rclR"
                     /locus_tag="ACHKA1_004325"
     CDS             13086..13940
                     /gene="rclR"
                     /locus_tag="ACHKA1_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO5681891.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGMDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQFAP"
BASE COUNT         3779 a         3081 c         3223 g         3961 t
ORIGIN      
        1 ggcgtattct acaattgcga caaaaacaac gatattaatc agtttatgac tgatttgttg
       61 tactttattc tctttcattg gtacttcctc gctttaaaaa agagcgcact tcgtaagtgc
      121 ccttatataa ataacgagtt tggtcaacca attttttgac atgtatcaca aatttgaata
      181 gatgtattac atcggctatc ttttattgca ccaacatcat tgatatatgt cgcctgaagt
      241 cagttccggg aatgagtctg atctcaagac tggcccagtc ctggcgttga ttggcgctga
      301 ggagcatatc gcatctcatc ataatgtcgt atctcctggg gtgttataca agatatcgtt
      361 gttggtaacc tgggcgagga attgagctct attaaaccgt caactatgcc ggatacatac
      421 tggattacac tgcaggcacg ccttatgaga gaacgtgccg cagtgacggg ttaattatct
      481 gaaagaattt gtgaggctgt atcggttact cattgatttg atagttttac tctcgggaga
      541 gtaatagata tttaatccat taacggaaac cagccagttc ctttcgatgc ctgaatttga
      601 tcccatagtt tatccggaat ggttaattca ggaacaagtt gtggattaag ggttgtttga
      661 atttcatggc ctctacctga ttcagctaaa gtcacccatt ctggatttat caccagccct
      721 ttaccgatcg caacaagtgg caggccagtg gaaattgctt cttgagcctg agacggtgtg
      781 cgtattttac ctgcagcgat taccggcact ctgcctgcaa tatgatttaa aatcaattca
      841 atagttcggg ggccatttgg cgactcaaca gggtagctat cgttaatact gaccagcgag
      901 gtatgtatat acgaaatacc ggatgaaatg agccgatcaa ggagtttata agtatcctct
      961 atccgcagcc cccctgttgc agattcttca ggagatattc tgtaaccgat ggcaaagggc
     1021 tttgtcgcat attcatagac tacatttttt acttcctgta atacggccag cgggaagcgc
     1081 atacgtcctt cgagatctcc tccccatcgg tcattacgct ggttaaatag aggggagaag
     1141 aaattctgta gaaggaatcc gtgcgcacca tgaagttcaa tgccatcaaa acctgcttta
     1201 atcgcccttt ttgtgacatc tccgaatgct ctaatggttt cctgaatttc gttttcagtc
     1261 atttccctgc tttgtaccac tctcttcata aagtcaccag attttacgct tgatgcgctg
     1321 gcactaataa catcgttatt tggtacaagc tccgggatgg ctttattacc ggcgtggaaa
     1381 atctgcaaaa tagccggagc acccccactt ttggctgctg ctgctaactt ttcaaggctg
     1441 ttaatgaaac ggtcgtcata cgcggcaaat tcatgagtaa acccaatgcc gcttggcgtg
     1501 acataggtac atcctgttat tactaaacca acattttgtg agcgacgttt ataaaattcg
     1561 agttcttgct cagagatggt tccatcagga ttcgctgacc aggtcgtcat aggagccata
     1621 acgatacgat tacgtagctt tattttttct gtcagcataa aaggagaaaa caatgatggg
     1681 tgtttatttg tcataacaat acctcaaatg tttatctgat tttgatgcgt atgttttaac
     1741 tggttagttg ttgccagctt tggaaccaag agttctggct ttatttataa tccctttaaa
     1801 gggtttctaa taattgagtt gctgcttttt tgttttttaa tgaaatttct ttttgagtaa
     1861 ttttattatt gttgtgtagt caataaagga taaatgtcag ggagataagg aatctccctg
     1921 taagattatt acaaatgtgt tttataaaaa ggaattaatt tatcgagtgc ctgtttcaca
     1981 ggttctggct tatcatacag gtcgtaatgt gaataacctt caaccacgac cagctcttta
     2041 tgttttgaag cagcgcggcc aataatttcg cagccgtcac ggtaggcacc aaatgcgccc
     2101 actctatcgc ccaccacaac cattattggt tgagttaaca ggacttctgc aagatgaaat
     2161 gcatcccaac ccacggcaac agtctgatgt gagaatagcg aacgatttac cccattaggt
     2221 gcatagcctc gtggcgaacg ataatattct gtggcttcaa aaaggtctat ttctgttaat
     2281 cccgcttcaa gagctgcatc cggagaagag gggagtaaat catctacgcg aagttttgcg
     2341 ccgttagctt cgtctgttct ttgttgcgcc attgcttcga gtgctccgat aggattaaac
     2401 gctgtaaatc cctcacgcat aagacgcccg tagtttgcac ccgtgacagt accaatagct
     2461 ttaattcgac gttctgtcat tgcggcatta atggcatatc cacctccacc acaaataccc
     2521 agtacaccaa tacgcccggc atcaacgtaa ggtaagctgg taaggtaatc aacgacaatg
     2581 ctgaagtctt ttactcgcat tgttggatct tccagataac gcggttcccc accgctgctt
     2641 ccctgaaaac tggcatcaaa agcaatgacg ataaagccag cttttgccag ggcggcacca
     2701 tacacactac ccgatgtctg ttctttgcag cttccaattg gatgggcgct gataatggca
     2761 ggatactttt tttcactatt aaaatcgtca gggaaataga tatcagcagc tatatcccag
     2821 taagtgtgtt ttatcgaaac agttttcatt tcaagctccg gggatttgcc taaacagatt
     2881 taaatatcat ttctattatc cgcaactggc attcgtgttc atatcgagaa cggcgtaata
     2941 gtatgacttg tattggttat catcaatggt ctaatatcta tcacagtgtt aagttaaggt
     3001 ttacaatgat gaaaatagag ccttcaattt tgccttctct tgcctggttt gcgctgattg
     3061 ttcgtgccgg cagtttttcc agagccgctt ctgaaatggg tattacgcgg gccgcattgt
     3121 cccagaattt aaaatctctt gaggaacgtc ttaacaccaa gctgatatac cgtacgaccc
     3181 gcaatatgtc gcttactgag gaaggacagc gtctgtatga agtgctggtg tcggctttgg
     3241 ggcaaattga tgatgctttg aaagatgttg gtgataccca acttgaacct acaggcctgt
     3301 tgagaataaa ttcctcccgt gttgcagcac ggatgcttgt tgagcctcat attggcgagt
     3361 ttcttactcg ttaccccaaa acaaagatag aactcattat ggatgacggt ttatctaaca
     3421 ttattgcgga cggctgtgat gtgggcatac gtcttgagca aggactggat gagcatatga
     3481 ctgccgttcc tgtttctccg cttattaaac tggttactgt ggcatcgcca gattatttaa
     3541 aagaacacgg tattcctgaa acgcctcagg aactgagtaa ccataattgc cttcggttgc
     3601 ggcataaaag tagcggtgca ctctctgcat gggagttttc taatgttgtg gggggtaatg
     3661 aagagttcga aatagaggtt tcaggtaagt atatttctaa tgatgacgaa agcatgatac
     3721 gaatggcgct gaatggaacg ggtatcattc aacatctgga ttttgcaatc gctgaacata
     3781 tcaatgcggg caagttacag ccgattcttg aggattgggc tgtttcattt cctggatttt
     3841 atatttatgt ttcatcacgg gtgagaatgc cctctaaagt tcgtgctttt attgatttta
     3901 tggtggagaa aagagtaaaa ctcgaaaatt gatgatatgt gtcagaatat cggtaagaag
     3961 aatggcacaa tcgtgaggat acgtttattc ataataaatc ttctgaaagt ttagaattat
     4021 tttcgataca gccagattat cattgttact aataatgtgg ctaaaataat agcatctatg
     4081 cccccccttc atgagtgtct gtgaagagga tgtttctaca aggaaatcgc caatgctcaa
     4141 agaaaacttc aacgaactgc aaatctttct tgtggtggca agagagcgaa gttttaccaa
     4201 agcagcgggc aaactcggcg tctctcagtc tgcacttagc catgcaatga aggctctgga
     4261 ggaaaggctc aatatccgcc tcctgacacg gactacccgc agtgtggctc ccacagaagc
     4321 gggggagaga atcattgcct gccttgaacc gcgtattgac gagcttgaac aggaactgga
     4381 gtcacttatt caacagaacg gcacgccctc cgggaatatt cgtttatctg ccggggaaca
     4441 tgccgcgcga agtctggtct ggccgaagct aaaacctttt ctcagaaagt atcccgaaat
     4501 cagtgtcgaa ctggtggtgg ataacggttt tgtcgatatt gttgaaggcc gttttgatgc
     4561 cgggatacga ctgggtgaaa atgttgataa agatatggtt gcggtcagaa ttggaccaga
     4621 tatgcggatg gctgtggtgg gggcaccggc gtattttgct gcaaaccctg cgcctgaaac
     4681 gcctcacgag ctacaaaacc atcgctgcat caacatacgt ttgccaactg ccggtggact
     4741 ttaccactgg gaattcgagc gagaagggaa agcgcttcgc gttaaggttg atgggcagct
     4801 gacgtgtagt ccattgccag aaagaattga tgccgcgtta tcagggtttg ggatcgcttg
     4861 tgtccctgaa gacatggttc aggagtatat aacgtcaggc aagcttattc aggtgttgca
     4921 ggagtggtgt ccaacttttc ccggatatta tctctactac cccagccgca agcagcatcc
     4981 gcctgctttt gcgctgttga tcgatgcgct tcgctacacc gaataacggg tcctttgtcc
     5041 aggacccgtg tgggtattag cggcctacgc gtgcctggtg ttcaggcgag taacgttcac
     5101 cgacgatttt aattgtttcc agcgcctcgg ttatctgctg cgtatctttc tgcgaaagaa
     5161 caatgtcagc agcggccagg ttttcttcca gccggtgcag tttagtggtg ccagggatgg
     5221 gaacaatcca cggcttttgt gctaacagcc atgccagtgc gatttgtgca gacgttaccc
     5281 ctttctccgc agccagttca cctaacaagg tgaccagttt ttcattagct tcaatggctt
     5341 gtgctgcgaa acgcggtacg gtgctgcggt aatcatcttt gccgaaagtg gttcccggct
     5401 taatcgaccc ggtcaggaaa cctttaccta gtgggctgaa ggggacaaaa ccaatcccca
     5461 gttcctccag caacggcaga atttcctgtt caggctcacg ccaccacatg gaatattcgc
     5521 tttgcagggc ggtgacaggt tgtaccgcat gcgcacgacg aatggtttgt gcgccagcct
     5581 cggacagacc gaaatgtttg accttacctt ctgcgatgag gtctttcacc gttcctgcaa
     5641 catcttcaat aggaacatcc ggatcaacac ggtgttggta cagcaggtca ataacatctg
     5701 tcttcagacg acgtaatgat ccttcaacgg cttcacggat atgttccgga cggctgttta
     5761 aaatctgctg cttattatca tcgccgaagg taaaaccgaa tttagtggcg atcaccacgc
     5821 ggtcacgaaa cggttttaag gcttcgccga ccacttcttc attcagatac ggaccataca
     5881 cttcggcggt atcgaagaag gtgacgccac gttcaaccgc agcacgaata agttcgatag
     5941 cctgacgggt atcggtcgcc gggccgtagc cgtggcttaa gcccatgcaa ccgagcccaa
     6001 gggcggaaac ttcgagtccg gatttaccca gataacgttt ttgcattgaa tgaatacctc
     6061 ttttatcgcg gcttaaacat caagtttgcg gccagtcagc cattccacca ttgcgggatc
     6121 gcggtgagag aagaacgcgc tggttgcggt atccagggcg gtaatttgca gcatatcttc
     6181 aggactgagt tcaaaatcga gaatgttgat gttctcttcc atgcgttctt tgcgtactga
     6241 ttttgcaagc gaaacaatgc ctcgttggta gatccagcgc agaacaacct ggcctacgct
     6301 tttgccatac ttctcgccaa ttgccgtgag aacaggatgc tggaacagac catttttccc
     6361 ttccgcaaac ggtgcccagg cttctggctg aataccacgg ctttgcatcc acggaacggc
     6421 gtgtagctgc tgattgaaag ggttaacttc aacctggttt accgctggaa cgacgttatt
     6481 aaaggcgata aggtcagcca gtcggtcagg atggaagtta ctgacgccaa tagcgcgaat
     6541 tttaccggcc tgttgcagct cctccatggc acgccaggca ccgtggacat caccgtaagg
     6601 ctgatgaatc aaatacaggt caacgtaatc aagttgcagt cgattcagag agcgttcgaa
     6661 ctgggctttg gccccttcgt aactggtatc ctgcaaccag agtttcgtgg tgacaaagag
     6721 ttcgttacgg gcgataccgc tttgtttcag tgcgttcccg acctgggttt cattttgata
     6781 ggaggcggcg gtgtcaatca gtcgataacc ggtgttaata gcgtcaatga ccgctctttc
     6841 gcattcagcg gcatcggtca tctgaaatac accaaagccc aacaggggca tttcaatacc
     6901 gttgttcagt tttacttttt gcatggtgtt atccttccgt tctcgttcat gagaagcata
     6961 acgtaaaggg aaaagctcga ttagacggca ggatttgtca ggggttatga acgaaattca
     7021 taaatctgtt tgagtgttgc gatgggtagt gcaagttcga tatctccgca atttacagtc
     7081 cgatgaagga aaatgaatat ccataaaaaa tatatcggtt tatcctggcg tatataccta
     7141 cttcgacgta tttccaatag ttgctaatta aaggtaggtc attattattc actctgaata
     7201 atgaattatt cactgtccac agagtaagaa atataactta agtatctatt taatgacttg
     7261 cacaaaaagc taaattttca cccataaata aaaatataat cccgcgccca accacctgat
     7321 gagtggctac aggcactgga tatattaggt ggcggtgtac tttcttacat aaaggtattt
     7381 ccttttctgc ggaaaaggaa accgggaaat ccccggtttt tctgacaagc agacgccatt
     7441 atttgtgtct gcctatgttc gttaattcgt tcatcaggaa attatctcaa tgtcacgtta
     7501 taaaacaggt cataaacaac cacgatttcg ttattcagtt ctggcccgct gcgtggcgtg
     7561 ggcaaatatc tctgttcagg ttctttttcc actcgctgtc acctttaccc cagtaatggc
     7621 ggcacgtgcg cagcatgcgg ttcagccacg gttgagcatg ggaaatacta cggtaactgc
     7681 tgataataac gtggagaaaa atgtcgcgtc gtttgccgca aatgccggga catttttaag
     7741 cagtcagcca gatagcgacg tgacgctgac tgccagctcg gaaaatttag ttctggatcc
     7801 taaagggacg gcgaaaacta atgagcaagg acaggctgtt ttcaccggct ctaccactat
     7861 cgcagcgaca tatacactca cggcgaaagt ggaacaggcc aacggtcagg tatcgacgaa
     7921 aactgctgaa tctaaattcg tcgcggatga taaaaacgcg gtgctcgccg catctccaga
     7981 acgtgtagat tctctggtgg cggacgggaa gactactgca acaatgacgg ttaccctgat
     8041 ggcgggagtc aatcccgtag gaggaagtat gtgggtcgac attgaggctc cggaaggagt
     8101 gacggagaag gattatcaat tcctgccgtc gaaggctgac catttctcag gtgggaaaat
     8161 cacgcgtaca tttagtacca gcaagccagg tgtctatact ttcacattca acgcactgac
     8221 gtatggcggg tacgaaatga cgcctgtgaa ggtgacaatt aacgccgttg ctgcagagac
     8281 tgaaaatggc gaggaggaga tgccataatt tgaatagtat gaaagttacg atctaataaa
     8341 gaataatggt gttgaaatat tcaacgccat tattcactaa ataccttgcc tgatactgat
     8401 taaatagtat caggcaagga taaaactcaa taagagatag tgaccaacta aatcggacga
     8461 acgggaataa aaatttcgca aacaacatca ttgccatcct tttctacgaa atcttcattg
     8521 taataaaagc gctcaatatc gctgccgttc actctggcta accccctggc aggcaactct
     8581 atcagataga tgttacgcac ccagtcagga taattttccc agtttccttc ataacggaaa
     8641 gaggcataaa gtcctcgggt cgttgtccac tcttttggca tgtcagcata ttcacatacc
     8701 gtaccagcaa aagtttcaac cgtgagcaaa tcgaccgatt ttgttgacgg ttcaaacgtg
     8761 gagacgaaat agatatccgt cttaaaatta ttcatacatc tggctaaatg atgacgtcgc
     8821 agtttgactt cttcatccgg tgatttattc gacacttcaa tatcatattt aaatgaattg
     8881 ccaaaatagg tgtctcagga aagttgacta atctacactc cgtcttttgc tgttgacgta
     8941 ttaaaaaaga agggaagata tttgcgagat cccaataatc acggtggcgg tatacacggg
     9001 gagagcagcc gaataacttt ttaaattcgc ggctgaatga ctgctgtgaa tcaaaatgca
     9061 aagagagtgc aatatcaagc atagatttcg cggtgagccg gacaagaatg gcggcacgac
     9121 aaagccttcg tttgcgaatg tattctccca aaggcacatg catgaaattt cggaacagaa
     9181 gctggatgtt gcgtctgctg tagccagatt tctgtgcgat atcttcgatt gaaatagggt
     9241 gctcaagatt gcactcaatc cactccagga gctgctgtag aatcttctgc ctgatcatca
     9301 cattctccgt taattctttg tcatatcgca cattttcgtt atgccctggg ccctaagtgt
     9361 atcaaaaaca gcaagggcga aggcaaaagc actggtagcg tctgcttcgt tgtccccgaa
     9421 aatagagagg tttcagtcct acattattaa tgaatttttt gcataagtga tatcaaaatc
     9481 cacatactaa tttgaggtta cgttttaacg tagactcatt gttcatgcct aatgtgccta
     9541 atggagggac tgacagtgga attttccgta ttaagtaaca atctgaaaat gccgatgatg
     9601 ggatttggtg tttttcaggt taccgataaa aacgtgtgca aacagtcagt gttgaacgct
     9661 atccgcacgg gttatcgact catcgacact gcagcggtat acggtaatga agatgctgtc
     9721 ggtgaagccg ttcgtgaagc catatccgaa ggtttatgta cccgagaaga gttatttata
     9781 acgtctaaat tgtgggtgca ggatatgttg aatcaggata cggcagcagc aggtattgaa
     9841 gcatcattaa aaaaatcggg actagaatat ttcgacctct atttatttca tcaggctatg
     9901 cgcgattatt tcagtgcgtg gcgtgcactc gaagatgcct atgaagaggg caaattaaaa
     9961 gcgattgggg tttccaattt ctatcctcat gttctggcga acttttgtga aacggtaaga
    10021 gttaaaccga tggtcaacca ggtcgagttg catccctatt ttgcccaacc agaggcgctg
    10081 gcaaccatga agtattataa cgtgcagcct gaagcatggg ctccgttagg tggtggacga
    10141 cataaaccct ttgaaaataa tctgcttcag agtattgcag atgcccatca aaaatcgatt
    10201 tctcaagtca ttctgcgttg gaatattcaa cggggagtgg tcgttattcc gaaatcgaca
    10261 catcaacagc gtatcgaaga aaattttgct atctgggatt tctcactgac agagaaagaa
    10321 atggcacaaa ttagttcgct tgatttgggt tatgttgggg agtcggtaaa acattttaat
    10381 cctgaatttg ttcgtggttg tcttgctgta aaaatacatg attgatatta atcatgtatt
    10441 ttacctgaga cgacaagaat cttttaacag gggagtgata ttgatcttca ctctgtcata
    10501 tctccggtaa tatggcgtca ggcttcatca tcagaaaggg ggcctgacgc ctgaaaaagt
    10561 gaacaacaga cagtgttcgg attatcaata ttcagttttt aacgttgaac tggattcatt
    10621 actgcgttgt ttaagaattt cccgcgccga atctgccatt atcatgacag cacctgccag
    10681 catcagagta tctttcaata ccaagcgacc agcaccggat aaataaggga aaccatgatg
    10741 agcgtcaccc aatgcgggta cccatgcctc cggggtggtg attaaaaatg agagtgttac
    10801 caacggtgtg gtaaatgcca tcagccctcc caataaacct aaccagcgat tgacaggatt
    10861 agccaaaacc agcagagcaa taatcacctc cacgacgcca agaccgttgg aaaaaccata
    10921 ggtattattg gccgattgcc atgcccttgc ttctggtttg tattcgcctt cgtgagtcag
    10981 atactgttta tagtcttccg ggtgttcata aaagaacgac attagtggac tgtttgcgac
    11041 gaatggtgta atgctgtctg cctcgtaagg gacaaacttt aataacccaa tccacataaa
    11101 aacaatcgca atactcagac gaatcaatgt caggccaatt ttatctcccc gacttaacag
    11161 gtgcaggtat ttttccattg tgaacatcct tatttataca atactgctgt tgcggttgaa
    11221 ttattatcaa ttcgcatcga tgtgactttc caggatgaag cattatattt tctggctata
    11281 tcagcaatgg catcctccag aactgtcggt gatgctgata ctgacgagac gctgatatgt
    11341 ttcagaatta ttttatcatc tgcattggtg gaaaatgcca taacgccaga taatagtgtt
    11401 gcaaataaaa cggatttttt aaacatataa aataccatca atgaagtgat taagaagtta
    11461 tagagtaaca gggaattaat aagattcttt tctctgggac gccagaatat ttgttctggc
    11521 gtctgatttt gagtttattt gattaatgaa aatagatcat tgagtgattc gctcatcgac
    11581 ggatgagtaa atatctgatc gcgtaatatg ctataaggca gcccggcatc catcaccatt
    11641 ttcactatat taatcatctc gtgggagtca acacacagca gtgatgcccc taatatacgt
    11701 tgggttttat tatcaacaat cgcttttaat accccacggg tatcgttcat cactctggcg
    11761 cgcggaattg cagctacagg caatgtcacc acctgaatat cagcaccact ctctctggct
    11821 tgttcttctg tcataccaac cctggacaag ggcggtgtca taaatacgga ataaggcaca
    11881 tttttccgat catcagtact acgtttgcct tcacccagta actcatcacg tacaatgcgg
    11941 taatcatcca gtgatatgta agtaaattgc agcccgccgg taacatctcc catcgcccaa
    12001 atattgtctg cggtggtatg taatcgcttg tcaacgacaa ttcccccgcg ctcgtttact
    12061 gcgacaccgg cattttctgg atgtaacgaa gcggtagccg gttgacgacc ggaagctatt
    12121 aacagtgcat ccaccgccag ttgggcgtgc tcgctatgca cttgcacttg attttcatgg
    12181 tgactgattc gctccacatg ggcattgagg ataatatcga cgccctgatc gcgtaaaatc
    12241 gtcgcgatat tatcagcaat atcccgatct tcccgaggca aaaacagcga agctgcttct
    12301 aaaatggtta ccttgctgcc aaaattagcg aacatagagg cgaactcaac gccaatatat
    12361 ccgccgccca aaatacctaa atgcccaggc aattctttta gattaagtaa tccggtgctg
    12421 tcatatacac caggcgtggt ggtaattcca ggaattggcg gaaccacggt ttgtgcaccg
    12481 gtattaataa aaattttctc gccatgaatt tccagatttc cctcaggccg atgaacacgc
    12541 aggctatgat tattgataaa ctccgcctgg ccgttgatca cgtcgatatt gggcatatct
    12601 gcaagattat gaaaattctt attacgtaaa aaattaacca cttcattttt acgctgtatg
    12661 gcacggacaa aatctgtgtg ctgctgtgcg tcatgaacca atgttttggt tgggatgcag
    12721 ccgatattaa tacaggtccc gccatacatt gcatttgatt gttcgatgag agccacacgc
    12781 caacctgctt ttgccagcgt gacggctaat gtttttccag ccttgccaaa accaataatc
    12841 actgcctgat atttattcat gatgatttcc tgtcgaaacg cgttcatttg acatcatgac
    12901 tatagacgca aaagagcaaa taacatttct tcacaacgct tgatattttg tcttatcgtc
    12961 tggtattttc tttttcttaa tcagaatcac aaaacaaaaa tcatgattaa ctatttgatg
    13021 gtattagatt agttgtttac taagattgtt ggtgtttgta atcaaaaacc actcaggagt
    13081 ctgatatgga tgcccttagc cgtttgttga tgcttaacgc tccacaagga acgatcgata
    13141 agaattgcgt gttaggaagt gactggcagc ttccacatgg tgccggggaa ttgtcggtta
    13201 ttcgttggca tgcgttaacg caaggagcgg cgaagctgga aatgccgacg ggagagattt
    13261 ttacattacg cccgggaaat gtggtcctgc taccacaaaa ttcagctcat cgcctgagtc
    13321 atgtcgataa tgaatcgacc tgtattgtct gcggcactct tcggctgcaa cattcggcgc
    13381 gttatttttt aacgtctttg ccggaaacgc tgtttttagc accggttaac cacagcgtgg
    13441 aatataactg gctgagggag gcgatcccgt ttttacaaca ggaatccaga tcggcaatgc
    13501 cgggaatgga tgctctgtgt agccagatct gcgctacatt ctttaccctc gcggtgcgtg
    13561 agtggattgc acaggttaat actgagaaaa acattctcag tttgcttctg catccacgtc
    13621 ttggtgcggt aatacagcaa atgctggaaa tgccaggaca cgcctggacc gtcgaatcgc
    13681 tggccagcat cgcccacatg tcccgggcaa gttttgccca gcttttccgt gatgtttccg
    13741 gaaccacgcc gctggctgta ttaacaaagt tgcgtctaca aatagcggcc cagatgtttt
    13801 cccgggaaat gctccctgtt gtggtgatcg ctgagtcagt aggctatgcc agtgaatcat
    13861 cttttcacaa ggcgtttgtc cgcgagtttg gttgtactcc gggagaatat cgggaaaggg
    13921 tcagacagtt tgcaccctga ataaaaccgc cagaaatcag ggcaaagata atccgcattc
    13981 cgggagttgt gagcctttca actatttcta tttccaacgg tggtttgggc tttatatatt
    14041 tttt
//
LOCUS       DAZFKF010000116        14008 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFKF010000116 DAZFKF010000000
VERSION     DAZFKF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.118"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            144..653
                     /locus_tag="ACHKA1_004326"
     CDS             144..653
                     /locus_tag="ACHKA1_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO5681892.1"
                     /translation="MNLQRFDDSTLIRIFALQELHRLKERGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQNLDDYYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            650..2068
                     /gene="phrB"
                     /locus_tag="ACHKA1_004327"
     CDS             650..2068
                     /gene="phrB"
                     /locus_tag="ACHKA1_004327"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO5681893.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVELVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLITYHPSLCKHCPFIAWTERVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRNVPGKSVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(2218..3699)
                     /gene="dtpD"
                     /locus_tag="ACHKA1_004328"
     CDS             complement(2218..3699)
                     /gene="dtpD"
                     /locus_tag="ACHKA1_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO5681894.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            3970..4713
                     /gene="ybgI"
                     /locus_tag="ACHKA1_004329"
     CDS             3970..4713
                     /gene="ybgI"
                     /locus_tag="ACHKA1_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO5681895.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4736..5392
                     /gene="pxpB"
                     /locus_tag="ACHKA1_004330"
     CDS             4736..5392
                     /gene="pxpB"
                     /locus_tag="ACHKA1_004330"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO5681896.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5386..6318
                     /gene="pxpC"
                     /locus_tag="ACHKA1_004331"
     CDS             5386..6318
                     /gene="pxpC"
                     /locus_tag="ACHKA1_004331"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO5681897.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDQLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6308..7042
                     /gene="pxpA"
                     /locus_tag="ACHKA1_004332"
     CDS             6308..7042
                     /gene="pxpA"
                     /locus_tag="ACHKA1_004332"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO5681898.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            7078..7869
                     /gene="nei"
                     /locus_tag="ACHKA1_004333"
     CDS             7078..7869
                     /gene="nei"
                     /locus_tag="ACHKA1_004333"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO5681899.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEESQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7866..8912)
                     /locus_tag="ACHKA1_004334"
     CDS             complement(7866..8912)
                     /locus_tag="ACHKA1_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO5681900.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     VNLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(9061..10122)
                     /locus_tag="ACHKA1_004335"
     CDS             complement(9061..10122)
                     /locus_tag="ACHKA1_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5681901.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AVPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     PLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     APIDVGPFDTVVLFKINYN"
     gene            complement(10119..10850)
                     /locus_tag="ACHKA1_004336"
     CDS             complement(10119..10850)
                     /locus_tag="ACHKA1_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5681902.1"
                     /translation="MTFVKGFPLILLVASMCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALPPIQRLEPKAASQVRIMKQAATAKLPTDRE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSQQN
                     NQLTLKNPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            complement(10865..13324)
                     /locus_tag="ACHKA1_004337"
     CDS             complement(10865..13324)
                     /locus_tag="ACHKA1_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5681903.1"
                     /translation="MDAVNIYRLSVLSCLAMVTPPALAAEFNLNVLDKSIRDSVDISL
                     LNQKGVVAPGDYFVSVTVNNNKISNGQQIRWQKSGDKIIPCINESLIELFGLKSDFRK
                     KLPAIKECVDFSVFPEIIFTFDQANQQLNITIPQAWLAWHSENWTPPSTWNNGIPGFL
                     MDYNLFASTYRPQSGSSSNNLNAYGTTGLNAGAWRLRSDYQLSQSDSGDNREQSGAIS
                     RTYLFRPLPQIGSRLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVSEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGRPRSSMSHHTEDETFISHEVSWGMLSNTSLYGGMLLAGDD
                     YRSGALGIGQNMLWLGALSFDVTWADSHFDTQQDEQGYSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYIDHKYNDADAQDEKQTISLSFGQPITPLNLNLYANILHQSWWN
                     ADTSTTANITAGFNVDIGDWKDISVSTSFNTTHYEDKDRDNQIYFSISLPIGESGRLG
                     YDMQNNSNSTTHRMSWNDTLDERNSWGMSAGIQSDRPDNGAQVSGNYQHLSSTGEWDL
                     TGTYAANDYTSASASWSGSFTATQHGAAFHRRSSTNEPRLMVSTDGVGDIPIQGNIDY
                     TNRFGIAVVPFVSSYQPTTVAVNMNDLPDGVTVSENVVKETWTEGAIGFKSLASRAGK
                     DLNVIISDANGHFPPLGADVRQAEGGVSVGMVGENGHAWLSGVDENQQFTVHWGDQKT
                     CAIHLPEHLEDVTKRLILPCH"
     gene            complement(13374..13940)
                     /locus_tag="ACHKA1_004338"
     CDS             complement(13374..13940)
                     /locus_tag="ACHKA1_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5681904.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGNGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVVLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
BASE COUNT         3396 a         3472 c         3818 g         3322 t
ORIGIN      
        1 gtcaagtatt cgctgctttg atgatgatgt aaaatgattg tttttgaact ggatataaca
       61 gcttggtagg gaagaaagca taaccccaca aaattcattt accatcgtct acacttactc
      121 ctgtaaaccg ttcaggagca gtaatgaatc tacaacgatt tgatgacagc acccttatcc
      181 gcatttttgc tttgcaggag ttacaccgtc ttaaagagcg aggcttaacg cgcggggcgc
      241 ttctcgatta tcacagccgc tataaactcg tctttctggc gcattcccag ccggaatacc
      301 gcaaacttgg cccgtttgtg gctgatattc atcggtggca aaatctggat gactattaca
      361 accagtacta ccaacgcgta attgttttgc tttctcatcc tgccaacccg cgagatcaca
      421 ccaacgtttt gatgcacgtt cagggttatt ttcgcccgca tattgattcc acagaacgcc
      481 agcagctggc agcgcttatc gacagttatc gccgtggcga gcaaccgctt cttgcgccgc
      541 tgatgcgtat caaacactat atggcgcttt atcctgacgc ctggctttca gggcagcgtt
      601 atttcgaact ttggccgcgt gtgattaact tgcgccattc aggagtttta tgactaccca
      661 tctggtctgg tttcgccagg atttacgtct gcacgataat ctcgcgctgg ctgccgcctg
      721 ccgcaattcg tctgcacgcg tgctggcgtt gtatatcgct acaccacgcc agtgggcggc
      781 gcataatatg tcgccgcgtc aggctgaact tatcaatgct caactgaacg ggctacaaat
      841 cgcgctggcg gaaaaaggta ttcctttatt gttccgtgaa gtggatgact ttgtcgccag
      901 cgtcgaatta gttaaacagg tgtgcgcgga aaacagcgtt actcatcttt tttataacta
      961 tcagtatgag gtgaatgagc gggcgcggga tgtggacgtt gaaagagcgc tgcgtaacgt
     1021 ggtgtgtgaa ggatttgatg acagcgtgat cctgccgccg ggggcggtga tgactggcaa
     1081 tcacgagatg tacaaagtct ttacgccttt taagaatgcc tggctgaaac ggctgcggga
     1141 agggatgccg gagtgcgtcg ctgcaccaaa agttcgtagt agcggatcga taaagcccgc
     1201 gccatccatt acgctgaatt atcctcgtca gtctttcgat actgcgcatt tcccggtgga
     1261 agaaaaagcg gcgattgcgc aattacgcca gttttgccag aacggtgccg gagagtatga
     1321 gcaacaacga gattttccgg cagtggaagg caccagccgt ttgtccgcca gcctggcaac
     1381 gggcgggtta tcacctcgcc agtgtttgca tcgcctgttg gcggaacagc cgcaggcgct
     1441 ggacggtggg gccggtagtg tctggcttaa tgagctgatc tggcgcgagt tttaccgtca
     1501 cctgataacg tatcacccct cgttgtgtaa acattgtccg tttattgcct ggacggagcg
     1561 tgtgcagtgg cagagcaatc ccgcacattt acaggcctgg caggaaggca aaacgggata
     1621 cccgattgtc gatgctgcca tgcgtcagct taacagcact ggctggatgc ataacaggct
     1681 acggatgatt acagccagtt ttctggtgaa agatttgttg atcgactggc gcgaaggcga
     1741 gcgatatttc atgtcgcagc tgattgatgg tgatttagca gccaataacg gtggctggca
     1801 gtgggcggct tctacgggta ctgatgcagc gccgtatttt cgtattttca acccgacaac
     1861 ccagggcgag aaatttgacc gcgaaggcga gtttatccgc cagtggctac cggaactgcg
     1921 caatgtaccg gggaaatcgg tgcatgagcc gtggaagtgg gctgagaaag caggtgtgaa
     1981 gctggattat ccgcaaccga tagtcgagca caaagaggcg agagtacaaa cgttggcagc
     2041 gtatgaggcg gcgcggaagg ggaaataaca gacgcgtcag gcaatcgagc ccagatgccg
     2101 gatgcggcgt gaacgcctta tccagcctac aaatttgtgc aaattcaata aattgcagga
     2161 aatacgtagg cctgataaga cgcgtcaagc gtcgcatcag gcatcggtgc tggcctgtta
     2221 agactccagc gccagcgcgc ggtttctgaa tttcagcgcc tgatacagcc aaatcatcag
     2281 taccactcct acacacgcca gtgcgcccca ggtaatttga tcaaatactt caatatatgc
     2341 attgatggag tagttgatcg ccccggaagc atcaaacgaa gcctgcgatg tctgatcggc
     2401 aatcacgccc gccagatagt tcgcaatcgc gccggaaagc agcatataaa tgccggttaa
     2461 tacgccggtc acaccgggga tttcaatgcg cgtaatctgc gacatagcaa ccgggtcgat
     2521 aaacagttcc gcaaagccca tcaccgccag gcctaatacc atcagtggca gagaagagtg
     2581 accatacatt gcggaccagc gggcgcttaa ggtcagaatg cagaatccgg cgctcatcag
     2641 gccaaggcca agagcaaatt ttccccagat acgcacggta cgattacccg cgacactctc
     2701 ttttaccacc cacgccagga acacaccgca cagcataact gcgaaggcat taatcgactg
     2761 gaacatcgcg gtcggaacgg tataaccaaa catatcgcgg ttaacgaagc ggtcgatata
     2821 aaggctaatc gagctaccgc cctgttgtgc gaaggcccag aacaacatac tgaagaaggt
     2881 gagcgtcaca atcagcccca gctccttacg ctgtttctgg ttttctgctt tgcgataaat
     2941 ttttgccagt acacccaggc caatgatagt cgcgacaatc aaggcgtata ccgaccattc
     3001 tttccagaac agtacggtaa tcagcgcagg cgttgccacc agcagaacca gcagccatcc
     3061 ccagttcggc agcagaaagt ttgtcgcacg cagtactttt ttgttaacgc cgcgggtatg
     3121 tgtgaaatga cgattgccac ataagaaaat aaccagaccc gcgatcatac ccaccgccgc
     3181 caggccaaag cccatcgccc aactgtactc ttcctgggcg tagccacagg caataggtgc
     3241 gataatagac cccacgttac ccgccgcata catcagcgag aaaccgccat cacgacgcgg
     3301 atcggttggc tcatacagct cgccgagcag gcaactgaca ttggatttaa acagaccata
     3361 gccacaaacg attatcgcca gggacagata gaggaatgac ggatggatct cacttgcacc
     3421 caacaccaca tgaccgatcg ccatcaataa cgcccccagc atcaccgcca tgcgattgcc
     3481 gagaacttta tccgccaaaa agccaccgag gattggcgtg acatacacca gcgaacagta
     3541 ggcgctaaat aactcgtagg cgtgagtatc gttgtatttt agttgattgg tgagatagag
     3601 aatcagcagg gcacgcatgc cgtaaaagct aaaatattcc cagatttgca gcgcaacgac
     3661 atagtaaata gcgcgcggtt gtgatgcgtg tttattcata ataattccac aattgacggc
     3721 aacgtcacgt aatagttgcg cggcgtgctt gtgtttcctt aaagttaaaa ctttgataca
     3781 gatctgatta tttatgcaat atgctgtctg attgcataaa tatacattag ctgaagcgtg
     3841 atgatataaa caatttgcgt cttttggcgt cgggtttgtt tcttaatatg tcaaaagatt
     3901 ggctatcgac gaacaggcga ggtaacgcta tgttaacggt gtagctatca gaatacggaa
     3961 ggcgcgaaga tgaaaaacac cgaactggaa caactgatta acgaaaaact gaacagcgcg
     4021 gcgattagtg attacgcgcc gaatggtttg caggtggaag gcaaagagac ggtgcaaaaa
     4081 attgttaccg gtgtcactgc cagccaggct ttgctcgatg aggcagtgcg tctgggcgcg
     4141 gatgcggtca ttgtgcatca tggctacttc tggaaaggcg aatctccggt cattcgcggc
     4201 atgaagcgta accgtttaaa aacgttgctg gcgaatgata tcaacctgta tggctggcat
     4261 ttgccgcttg acgcacatcc tgagctgggc aataacgcac agctggcggc gttactgggg
     4321 atcacggtca tgggcgaaat tgagccgttg gtgccgtggg gcgaactgac catgcctgtg
     4381 ccgggactgg agctggcttc ctggattgaa gcgcgtctgg gacgtaagcc gttatggtgt
     4441 ggcgataccg gacctgaggt ggtccagcgc gtcgcctggt gcacgggcgg cgggcaaagt
     4501 tttatcgata gcgccgcgcg ttttggcgtg gatgctttta ttactggcga agtttctgaa
     4561 cagaccattc attcagcccg cgagcaggga ttgcattttt atgctgcagg tcaccatgcc
     4621 actgaacgtg gtggtattcg cgcattgagc gagtggctga atgaaaatac cgatcttgat
     4681 gtgaccttta ttgatattcc taatcctgca taacgaataa tcagagggat cgaaagtgca
     4741 acgagcgcgt tgttatctga taggtgaaac ggcggtagtg ctggaactgg aaccgccggt
     4801 gacgctggct agccagaaac ggatctggcg actggcgcag cgtctggtgg atatgccgaa
     4861 tgtggttgaa gccattcccg gcatgaacaa tatcacggtg attttgcgta atcctgagtc
     4921 gctggcgctg gatgccatag agcgtttgca acgctggtgg gaggagagcg aggccctgga
     4981 acctgagtct cgctttattg aaattccggt ggtttacggt ggtgcaggcg gaccggattt
     5041 ggcggtggtc gcggcgcatt gcgggttgag cgaaaaacag gttgttgaat tgcactcctc
     5101 cgtggaatac gtggtctggt ttttaggttt tcaaccgggc ttcccgtatc tcgggagttt
     5161 gccggaacaa ctacacacgc cacggcgcgc tgaaccgcgc ttgctcgttc cggcaggttc
     5221 tgtcgggatc ggcgggccgc agactggtgt ttatccgctg gcaacgccgg gtggctggca
     5281 gttgattggt catacctcac tcagcctgtt tgatccggcg cgtgacgaac ccatcttatt
     5341 acgtccggga gacagcgtgc gctttgtacc gcaaaaggag ggagtatgct gaagattatt
     5401 cgtgcgggca tgtataccac tgtgcaagat ggcggtcgtc acggttttcg ccagtcgggt
     5461 atcagccact gcggcgcact ggatatgcct gcgttacgca ttgctaacct gctggtgggt
     5521 aatgatgcca atgcccccgc gctggagatc acgctcggtc agttaacggt tgagttcgaa
     5581 actgatgggt ggtttgctct gacgggtgcc ggttgcgaag cgcggctgga tgataatgcc
     5641 gtctggaccg gctggcgatt gccgatgaaa gcaggccagc gtttaacgct taaacgcccg
     5701 cagcacggga tgcgcagtta tctggcggtc gcgggtggta ttgatgttcc gccggtaatg
     5761 gggtcatgca gcaccgatct caaagtgggg attggcgggc tggaaggccg tttactgaag
     5821 gatggtgacc aactcccgat tggcaaatcg aagcgtgatt ttatggaagc gcagggcgtt
     5881 aaacagctgc tgtggggcaa ccgcattcgc gccttgccgg ggccggaata tcatgagttc
     5941 gatcgcgcct cgcaggatgc attctggcgt tcgccctggc agcttagccc gcaaagtaac
     6001 cgcatgggct atcgcttaca ggggcaaatt ttaaaacgca ccaccgatcg cgaactgtta
     6061 tctcacggtt tgttaccggg tgtggtgcag gtgccgcata acgggcagcc cattgtgttg
     6121 atgaacgacg cacagaccac cggtggttac ccgcgtattg cctgtatcat tgaggctgat
     6181 atgtaccatc tggcgcaaat tccgctcggt cagccgattc attttgtcca gtgttcactg
     6241 gaagaggcac tgaaagcgcg gcaagatcag caacgttatt tcgaacaatt agcgtggcgg
     6301 ctgcacaatg aaaattgacc tgaacgccga tctgggcgaa ggctgcgcca gcgacgcaga
     6361 gctattaacg ctggtttcct ctgccaatat tgcctgtgga tttcatgcag gcgatgcgca
     6421 aaccatgcag gcttgcgtgc gtgaagcaat aaaaaatggt gtcgcgattg gcgctcatcc
     6481 gagttttccc gacagggaaa attttggtcg cagcgctatg cagctgccgc cagaaaccgt
     6541 ttacgcccag acgctgtatc aaattggcgc gctggcaacg attgcccgtg cgcaaggcgg
     6601 cgtaatgcgt catgtcaaac cgcacggcat gttgtacaac caggcggcga aagaagcaca
     6661 actggcagac gccatcgcca gagcggtata cgcttgcgat ccagcattgg ttctcgttgg
     6721 gctggcagga agcgagctga ttcgtgcagg caagcaatat ggcctgacaa cgcgcgagga
     6781 agtgtttgcc gatcgcggtt atcaggctga cggctcgctg gtgccgcgaa gccagtcagg
     6841 cgcgttgatt gaaaacgaag aacaggcgct ggcgcaaacg ttggagatgg tgcaacacgg
     6901 cagagtcaaa agtatcaccg gcgaatgggc aacggtcacg gcgcaaacgg tctgcctgca
     6961 tggcgacggc gagcacgcac tggcgttcgc ccgccgacta cgctctgcat ttgccgaaaa
     7021 ggggattgtt gtcgcagcat aaccccgatt aataaagaat gaaaaaagga tatcaccatg
     7081 cctgaaggcc cggagatccg ccgtgcagcg gataacctgg aggcggcgat caaaggcaaa
     7141 ccactaactg atgtctggtt tgccttcccg cagttaaaaa cttatcaatc acaacttatc
     7201 ggtcaacacg ttacccatgt ggaaacgcgt ggtaaggcgt tgttaactca tttttccaac
     7261 gacttaacgc tctacagcca taatcagctt tatggcgtct ggcgcgtggt tgataccggc
     7321 gaagagtcgc agaccacgcg agtattgcgg gtaaaactgc aaacggctga caaaaccatt
     7381 ctgctttata gcgcctcgga tattgagatg ttgaccccgg aacaactgac cacgcatccg
     7441 tttttacaac gcgttggtcc cgatgtgctg gatccgaatc tgacgccgga ggtggtgaaa
     7501 gaacggttat tgtcgccgcg ctttcgtaac cgtcagtttg ctggattact gctcgatcag
     7561 gcgtttctgg ctgggcttgg caattatttg cgtgtggaga tcctctggca ggttgggttg
     7621 actggaaatc ataaagcgaa agacctcaat gcggcgcaac tggatgcact cgcacacgcg
     7681 ttactggata ttcctcggtt ttcttacgct acgcgggggc aggtggatga gaataagcat
     7741 catggggcgc tgtttcgctt taaggttttt catcgtgatg gcgaactgtg cgaacgctgt
     7801 ggcggcatca ttgagaaaac cacgctgtca tctcgcccgt tttactggtg tccgggctgc
     7861 cagcactagg ccgtacgttt cggcgcatag gttgaaataa accgcgcaat ggcaggccct
     7921 gtcagcaaaa tactgaacag gcgcagggtt tgcatcgcca taatgagcgc catatcggca
     7981 ttgctccctg cggcgatgac cgccacggta tcaagcccgc cggggctggt ggcgaggtag
     8041 gcagtcataa aatcaatgtg cataaaccgg gtcagccccc acgccatacc cgcacaaata
     8101 gccagcagag caaaaatcga cagcaggatt tgtggtagcg ggcgtagtgc ccgcagtaag
     8161 atttgtttat cgaaaccaag accaatccgc cagccaattg ccatatacgc catcgccagc
     8221 agccattccg gtagttcgat ggtgatgagc tgaccagact ggagcactgc gcccgccagc
     8281 atcgggatga gcatcgtgcc ggaaggaaga cgcagcaggc atcccactgt accggcaacg
     8341 accgcgagca aaatagtaag caggagatta atgctcactg gcgggaacca gacgatatgc
     8401 tggttaaccg cttcagcgtt atcgcccagc atcattcgcg tgaccagaac tgcggccccc
     8461 gcgacaaaca gcactcgcag atactgcata aacgccacca ggcggatatc tgcgccgtaa
     8521 tcctgcgcca tcgcgaccat tgccgccgcc ccgccaggcg aggagcccca ggcaccagta
     8581 tttccgggca gtgagctata gcgcaccagt agccatccca cgatagcgct ggaaagcaac
     8641 gtcaccagta aaatagctaa cacaatcggc cagttaaccg ccagcgtggt caggatcgaa
     8701 ccggtcaagt tttgcgcaat catgcagcca agaatggcct gcgcggcgag aaaagcggag
     8761 cgggggagtt gcaggttaac cccgcgcata ctgaagatga tcccggcgat catcggccca
     8821 agtaataatg ccgccgggag atgtaccgcg aggaaaccaa tagaaaggag aagtgatagc
     8881 acacataaca ttccccactg caaaactggc atcctttgct ccctcattat ttactctttg
     8941 gtgtatcgat acagcataaa gaatgccggg catggaaaaa ataacggtaa tcaataaaag
     9001 ccggaaaatt ggcaattaac cgaagaagcc ctctccggct ggaaagggca ataggctaaa
     9061 tcagttgtag ttaattttga aaagtaccac tgtatcaaag ggaccaacgt cgatgggcgc
     9121 acccggcgtg gatgacaatt ctgccgtgta ggttttttca tacactaaca ggtcgctggt
     9181 aaaatcggca tactcactgt atttgttgaa agtatagggt gaggcatttt catccaacaa
     9241 tcgaagcttc aggccattgc caatgagcag tgatttatct tcgtcgataa ggggttcatc
     9301 ggtataaaac gaagagctga ctttaaaacc gtcggtacac tggtcgtttt gtgatttggt
     9361 tgtagttatt ttaaattgct gcgacatcgt atggcgacga atatccagca cgttaaatcg
     9421 gccaaaatca acgacctggg tttcaggtga gatagcaaag ttgacgctgc aatccagcac
     9481 gcggatattt tccagaccgg taatgtgata tttcagattc ttcgcggtag ggtcttcgtt
     9541 cacgctgcct gcgccatcaa actgtacgac gatgtaatcg ctgagtttgc tctgataatc
     9601 atggggcggc atggcgcgaa ctttaacgta aagccgcata cgcgccacaa acgtccgcga
     9661 tttgtgcata acggttggat cgccggagca aagtttattt tgccagccca tcgcaatgat
     9721 ctgctccggg gtgtaaatat caatattctt actgtcgatg cactgattcg tgtcgatgcg
     9781 gcttttccca aggcttgcgt catagtccac gccttcatag gttacgccca attgataata
     9841 gggatcctga ataccggggt aagggtttac ccaggcgtag acgtgctcgc tttcaaaatt
     9901 accgtttgtg ttgttgtcac agtagaccgg aatttttata tcgtcagatt cccagatttt
     9961 atcgccgggt ttggaattgg ccggaacggc gaagggcata atagcttccg atttttctac
    10021 agcgccgttt gcggtaccaa aatagcagtt cagcgccagc gccgctttta gcggcaaaaa
    10081 aatcagacag atggcgagca acacgccttt ggcgaatctc attttttatt ctccaccggc
    10141 tgcaggcggc attgccgctg gcaggagagc gtgttcatac gcaatgcgcc gtagtcgtcc
    10201 atgtagccaa gataaaactg cttgccgcta tagctgcccg tgctggtggt gaccgaactg
    10261 aacggtgcga ccatcgtgct tttaaagcca ggcagcacgc ctttctcatc ccgcccaaga
    10321 taggcaatag tcagatagta tccggtcggg tttttcaggg taagctggtt attctgctgg
    10381 ctaacctgta gctgttgctc aacatgatcg cccatttttt tgcgtaatgc ggcgggtcgc
    10441 cagaatagct taatgcgact ttggacggcg acttgcagga tcgcatggcc ctcgctgcct
    10501 tccgggacag gcggaatttc acgcaggtta tagtaaaaga gagattcgcg atcggtgggt
    10561 aatttcgcgg tggcggcttg tttcatgatc cgaacctgcg aagccgcttt tggctccagt
    10621 cgctggatag gcggcagggc gacgagaaat tcatcgcttt tctctccctt ttcattttct
    10681 atccatgaat aggcgaggta ggggagttta tcgctgcggt tttccacgcg caggctggtt
    10741 gctttatctt tgctattaaa gatgatgcgc gtgcggtcag gctgcactgc gccgtggctg
    10801 cacatactgg caaccagaag aatcaaaggg aatcctttaa caaatgtcat cgtcttttcc
    10861 ttaattaatg acagggtaaa atcaggcgct tagtcacatc ttccagatgt tccggcagat
    10921 gaatggcaca cgttttctgg tcgccccagt gcacggtaaa ttgttgattt tcatcgaccc
    10981 cactcagcca tgcgtgaccg ttttcgccaa ccatcccgac gctgacaccg ccttcagcct
    11041 ggcgaacatc ggcaccgagc ggcgggaaat gcccgttagc atcgctgata atgacgttga
    11101 gatctttccc ggcgcgggac gccagcgatt taaagccgat ggcgccttcg gtccaggttt
    11161 ctttcaccac gttttcggaa acggtaacgc cgtcaggcaa gtcgttcata ttgaccgcca
    11221 ccgtcgtagg ctggtagctg gaaacaaacg gtaccacggc aataccaaag cggttggtgt
    11281 agtcaatatt gccctgaatc ggaatatcgc ccacgccgtc ggtgctgacc atcaggcgtg
    11341 gttcattggt ggaactgcgg cgatggaatg ccgcgccatg ttgagtcgcg gtaaacgagc
    11401 cgctccaact ggcactggcg gaagtgtaat cattggcggc ataggttccg gtcaaatccc
    11461 attcccccgt cgaactgagg tgttggtaat tgccgctgac ctgcgcaccg ttatccgggc
    11521 gatcggattg tattccagcc gacattcccc aactgttgcg ttcatccagg gtatcgttcc
    11581 acgacatgcg gtgggtggta ctattactgt tgttctgcat gtcataaccc agtcgaccac
    11641 tttccccaat cggtaaggag atcgaaaagt aaatctggtt gtcgcgatct ttatcttcat
    11701 aatgggtggt gttgaacgag gtcgagacag aaatatcttt ccagtcgcca atgtcgacgt
    11761 taaaaccggc ggtaatgttg gccgtggtgg aggtatccgc attccaccag ctctgatgca
    11821 ggatgttggc gtagagatta agattcagcg gagtaatagg ctgaccgaag gagaggctga
    11881 tggtttgctt ttcatcctgc gcgtcggcgt cattgtattt gtgatcaatg tagttagcgt
    11941 agctgtgaaa atggcgatcg gagaagcgat aggctgccaa tgagatggtg ctgttggtcg
    12001 catcaacctg tttgctgtag ttaaagcggt agctgtaacc ctgctcatcc tgttgagtat
    12061 caaaatggct gtcggcccag gtcacgtcaa acgacagcgc ccccagccag agcatatttt
    12121 gcccaatacc cagcgcgccg gaacgatagt catcgcctgc gagaagcatg ccgccatata
    12181 gtgaagtgtt ggagagcatc ccccaggaga cttcatggct aatgaaggtt tcatcctcgg
    12241 tgtggtgcga catagaggaa cgcgggcgac cggcagccag tttatagcgc acctggccct
    12301 gacgcgtcag gaacggggta gaggccgctg aaacctggaa gttattcacc cgtccatctt
    12361 cttcgctcac tttgacatcc agtgtacctt gcactgactg attcaggtca tcaataataa
    12421 atgggcctgg cggaactttt ttctggtaaa tcacgcgacc agaatggctt atcgtgaccg
    12481 tggcgttagt ctgtgcgatg ccgctgattt gcggcgcgta gccgcgtaac tcccagggga
    12541 gcattcgatc gtcgctggct aacgccgcac cggtatagga gaaaccatca aaaatgttag
    12601 agctaaaatc ggtttcgccc agggttaatc gggaacctat ttgcggtaac gggcggaaca
    12661 ggtaggtccg tgaaatcgcc ccggattgtt cacggttgtc gccgctatcg gactggctaa
    12721 gctgataatc gctgcgcaga cgccatgccc cggcgttgag gcccgtggtg ccataggcgt
    12781 tcaggttgtt gctgctgctg ccgctttgtg ggcgataagt gctggcaaac aggttgtaat
    12841 ccatcaggaa gccaggaata ccgttattcc acgttgaggg tggcgtccag ttttctgaat
    12901 gccacgccag ccatgcctgc gggattgtaa tattgagctg ttgatttgcc tgatcgaaag
    12961 taaagatgat ttcagggaag acactaaaat cgacacattc ttttattgcc ggtaattttt
    13021 tacgaaagtc agatttaagt ccgaagagtt cgatcagcga ttcattgata caaggaataa
    13081 ttttatcgcc agatttttgc cagcgaattt gttgcccatt actgatttta ttattattaa
    13141 ccgtaacact aacaaagtaa tcgccgggag ctacgacgcc tttttgattg agcaacgata
    13201 tatcaacgct atcccgaatc gacttatcga gaacgttaag gttaaattca gctgccagcg
    13261 caggaggggt taccattgcc agacaggaaa gaacagagag tcgataaata ttcacggcat
    13321 ccatacctga acaatattcg caaaaggcgg ctaatcagcc gccttaaaaa ggattattgg
    13381 taatccagaa cgtaagttgc gttagcagtc acggcccccg cggtaaccga aactgcgtta
    13441 tccatctgtt ccatccaggc aaagaagttt aacgtttgtt ctgttgaact tgccaataag
    13501 tcaattgttg gcgcttctgt tcccaataca acaaatgatt catctttgtt catgagacgt
    13561 acgccaacgc cggtagcctc gccagcactg atgttactaa ggagcggcgt tgcacctgtc
    13621 gttttagctg tgctgtcgaa ggtcacgcct acctttgaga ccacgccacc gttgccatca
    13681 tcccagcctg caatatcaca gttgattaag cgaatattga caggtttttt ctcgctatga
    13741 tgttctcgat ttatgtggga tgtggttact tcacccaggt caatgttcat atcaaggtca
    13801 tcagggtgaa tttcacaagg tgcttcaata acttcacctt taaagtggat ttcaccagat
    13861 ccttcatctg cagcaaaaac aggtgaagcg aatagtagag atatggccag ggcggccagt
    13921 gttttttgtc ctttaaacat acatgaatcc ttcatgggct atgataaagt atactaatta
    13981 ttccgacaga atagtggttt tttgttca
//
LOCUS       DAZFKF010000117        13947 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFKF010000117 DAZFKF010000000
VERSION     DAZFKF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.119"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1550)
                     /locus_tag="ACHKA1_004339"
     CDS             complement(<1..1550)
                     /locus_tag="ACHKA1_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681905.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFVLLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRSIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAIRQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLDNAWYERNMAGFNEKLRES
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEAS"
     gene            complement(1671..4793)
                     /gene="agn43"
                     /locus_tag="ACHKA1_004340"
                     /gene_synonym="flu"
     CDS             complement(1671..4793)
                     /gene="agn43"
                     /locus_tag="ACHKA1_004340"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO5681906.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRTGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGIARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHKYGLALNTVINEGGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGEASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADS
                     GAAVSGTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGSLA
                     GTTTLNNGATLTLSGKTVNNDTLTIREGDALLQGGALTGNGRVEKSGSGTLTVSNTTL
                     TQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVLGNML
                     QAGAFNYTLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASS
                     GNNDFRARGWGWLGSLETGLPFSITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHG
                     SAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            complement(5127..5999)
                     /locus_tag="ACHKA1_004341"
     CDS             complement(5127..5999)
                     /locus_tag="ACHKA1_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO5681907.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILLCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTTESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            7340..8830
                     /locus_tag="ACHKA1_004342"
     CDS             7340..8830
                     /locus_tag="ACHKA1_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO5681908.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTSWKNKEEILKAQKKALLSKLNKELRKGADESETLRQLEALQKNREEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGGSLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLDKLNACYSQKLIRWHEQSFGL"
     gene            complement(8952..9524)
                     /locus_tag="ACHKA1_004343"
     CDS             complement(8952..9524)
                     /locus_tag="ACHKA1_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681909.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(9610..9816)
                     /locus_tag="ACHKA1_004344"
     CDS             complement(9610..9816)
                     /locus_tag="ACHKA1_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5681910.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
     gene            10673..10897
                     /gene="hha"
                     /locus_tag="ACHKA1_004345"
     CDS             10673..10897
                     /gene="hha"
                     /locus_tag="ACHKA1_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO5681911.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVSSHTRYKLTPAELEA
                     FNSAVDHRLAELIMNKLYDRVPASVWKYVI"
     gene            11532..12101
                     /locus_tag="ACHKA1_004346"
     CDS             11532..12101
                     /locus_tag="ACHKA1_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO5681912.1"
                     /translation="MTYKYNPFWQQRIRETVLRALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKRREGKRVHPTILHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGCYATLANFPAWPVVWLERGDDTGFQ
                     QVLERADYLAKESTKAYGTGERNFGCSRG"
     gene            12268..12651
                     /locus_tag="ACHKA1_004347"
     CDS             12268..12651
                     /locus_tag="ACHKA1_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO5681913.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHAQQCPYVRPDRREVQLVKRLRQFVPDALPVVRKASWYCRQC
                     HHDYYGERYCTHCQTGRFSEEGGAE"
     gene            12648..13073
                     /locus_tag="ACHKA1_004348"
     CDS             12648..13073
                     /locus_tag="ACHKA1_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO5681914.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCGRLF
                     PGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGSAST"
BASE COUNT         3280 a         3521 c         3683 g         3463 t
ORIGIN      
        1 gacgcttctg cgggatcggt tacaccgggt actttacctt cgcggatctt attcttcatg
       61 gtttccacag cgccggcata ctgatcacgg gggacttcca gttgcatggg accatgattt
      121 cccatgtaac gatactgtcc gttttgtccg tcgaatgccg caccgacgct acgggctgca
      181 gttgaacaat atttcgtctg gatttctgtt ccgctgacca gtctgtctgc tccatttctg
      241 gcattattat caccgaggat ccgtgcgttt ttcagacgga ctctgtcgac aatatggttt
      301 ccccgttcag cggcaaaccc atgtccctgc cctccggaag caaacatttt ctcatttgta
      361 ctccaggttt ccggcagttt catgagaatc agaccattcg ccgttgcatc ggaaataaaa
      421 ccggtgtaag aacgttcata aatgtcctga cacagtgttt ctgtttcgcg acaaagagag
      481 gagcagtacc attccggatc cggcttatga ttttccgggg catgatgtac ttctgtcagc
      541 ccgcaggagg cacagatacg aagctcatcc acgtggtgga atgatacaaa acggcggcct
      601 tctgaaaagg ggtaccagtc accgccgtcc aggcaacgat cgagaaaatc gtcattcgct
      661 tccggtgata aattcaggcg gttccggaag agggttttca gttcatcagg ggtaaatgac
      721 aggctctctc tcagcttttc gttgaaaccc gccatgtttc tttcatacca ggcattgtcc
      781 agtagccagt ttccgtctgc cagttcaaat tccctcagtt ctccccggga aatccgttgt
      841 gaaatgagtg tcagccagat gcgctccgtc agcgtttcag gaaggctgta atcgttttgc
      901 aaactgctca gccacgattt actgttgagc accggttgtt cttccagtgt ctggcggatg
      961 gcactggtca gtcttttcag cgcctggatc tggctggccg cccatagcag cgtcactaat
     1021 gcagccagcc agtacaacaa cccgccccac cacatcagtg agggtgaaat atcgaactgc
     1081 aatgcgatca atggtgcgta tgcagaacac gccataagga aaaaagcagt ataaaaacag
     1141 cagtaatacc cgttaatttc cttgctgata tcttcaggtg tgagcccgtt ctgctgaatg
     1201 cttcttatct gcacgggaag agccagcaga ccagcggcga gagcggtggt cagaataaat
     1261 tttacagagt caaaagtatg agcctccagt gcgatatgtt taccatcaag tccccacatg
     1321 aagctgatga tcattccggc aatcgtcaga aatacgcctg ccagccagcg aatatggaat
     1381 aaccgggtta tgcgttcagt gatatagcca gccagtaaag ccgggccgaa cagacacagc
     1441 aatacgaatc ctgccagcgc agacgccgtg ctggttaatg ctctgaacaa aaggcgaaga
     1501 atggcaaatc ctgcgatcag caggatcagc gcgccgacta tctgtaacat agtcactccg
     1561 tgatgaatat catgttattg tgaatgccag tgaatgtggc actgaaacgt tttcccccgt
     1621 ccgggtcccg gtcatgatga ccgggaccac agagaggcaa tgcagaatta tcagaaggtc
     1681 acattcagcg tggcctgact gttataacct tcagcgctgc tgccgttgat gctgtgggca
     1741 taaccggcct gaacgcccag ggtgatattt tcccggacac gggcttccag tccggcctgc
     1801 agatccagtg aagtgccatt ctgtgaggga gagaacgtca tcccgctgcc tgcggtggag
     1861 gtccccacac gcatatcacc ccgggagctg aaggtgcgga taacggaagg ctgtacccac
     1921 cagttcaccg gcagttcacg cacactgtgt tttgcactgt cacgcagggg ggcacgggat
     1981 gaggtgcctt caccaaaggt catatcgttg tggctgccca gacggaaacc ggcacgcaca
     2041 tgctgtgcac tgccatgccc gaacttcaca taaccggcgt tgtccttacc gtcatccagc
     2101 gaaagcccct gccaggtgta ctgcagccgt ggctccagca tcaggttgtc agtgatactg
     2161 aagggcagac cggtttccag tgagcccagc cacccccagc cccgtgcgcg gaagtcgtta
     2221 ttgcccgatg acgctttcat actgtggcgg gttccctgtg ccacaatgtc agcccacagg
     2281 ccggaggagg tgtgtgtcag gttcatatat ccgcccaggc tgccggcatc atcccggacc
     2341 gtgccggcgc gggagccgtc atcatcctta acatcaacgg aagaatggcc tgcagcacca
     2401 tacactccag tcgtcagtga cataccggcg acctctgttc tgagcaggtc accctccaga
     2461 cggacgaagc catagctgcc gctgctttcc ggcgtggccc cacgggcaat accgccgttg
     2521 ttatcgtgac cgagatggcc gccctgaatg ctgagacgga cgctgttatt ttcaccgttt
     2581 acaccggtct gatggctgcg ggagcctgcc agaatccggt catagtccat tgcctgtgtc
     2641 agcatggagg cgtacagggg gacttcagca cgataacgtt cttcactgcg cagataccag
     2701 ctctcatcac tgtcccggtt gagggtgtag ttaaaggccc cggcctgcag catattcccc
     2761 aggacaaaag ccccttcctc cgtggtggca ccgttaatgg cctcaaccac ctgaataccc
     2821 ttaccggtgg tcgccagccc cgtaccactg ttgccggcgt tcaccagatt caggatggtt
     2881 tttccggttg ccctgccacc gtcaatgacc agtctgtcag cattgttctg cgccatatcc
     2941 gggcgtacac gcaggctgat ggtgccattc tgcccgttca ggtttttcac ctgcagggtt
     3001 gtcggaacga acttccctgt gcgggcggag gtgaaatgaa tctgtccggc atggctgagg
     3061 tcatccacca ccgactgaac cgtggcgtta tcggggatat tccaggtggc accggatgtc
     3121 agggtgacat tcgtggggtc aatggcaccg ttcagcactg tgctgccggt cagcttcagg
     3181 gccgtgccgc gctgagcgat gacatccgtg gtgacggtac tgtcgttcag cgtcagcgtg
     3241 ccttcattca ggttgacggt tttctgggtg agtgtggtgt tgctgacagt gagtgtgcca
     3301 cttccggatt tttccaccct gccgttaccg gtaagagcgc ctccctgcag gagtgcatca
     3361 ccttcacgga tggtcagggt atcgttattc accgttttcc cggaaagggt aagcgtggca
     3421 ccgttattca gcgtggtggt gcccgccagc gagccccctc tggcggtgaa cagtccgcca
     3481 tttaccgtgg tatccgtggc cgtatcaccg gcgttcagtg tgaaggaact gcctttttcc
     3541 agcatcaggg catccgcctg accgcctccg atacggaatg ccgttccgtc gctccgggta
     3601 ccactgacag cggcaccgga atcggccagc agtaccccgc cattccccat ggatacggtg
     3661 gtggcggcac caccgttgcg gacatccagc gttccgccat catccacacg ggtattagtg
     3721 gctgtgtgtc cgctcagcac atccagacgt ccgccatttt ccagtacgac attatcagcc
     3781 ttacccgcca caacggagaa tgctcccagg cggtttgtac cggtgacggt tgcagctgta
     3841 ctggtaacca gtgcaccgcc cgtgttctgg gtgacatcag acgcttcccc gccggcattc
     3901 acctgcagct ttcctttctg gttgatggtg gtatgcgcgg cagttcctcc ttccttaatc
     3961 acctgccagc ccccctcgtt aattaccgtg ttcagcgcaa gtccgtattt gtgcacatac
     4021 tggtttcctc cctccagccg ggtgttatgt gcctctccgt gtaccgtctg gtcaccgccg
     4081 gcgtaaatga gggtgtcccg tgctatcccc gaagaccaga taacctgccg gccatttttg
     4141 ttgatggtgg tggattcagc cgtccctccg accatctgac cgctggacac attttcagac
     4201 tccggaccac cttctgcacc ggtgttgacg atggttccgg ttgccagtcc gccatgtttt
     4261 atggtctggt aaccatcctg gttaataatt gtaccggcag ctttccctcc cccgtgtacc
     4321 cactgctcac ctctgttatc cagcgtggtg ttcaccgcca gtccgttaag gctctgccct
     4381 ccgccatcac gaataaccgt atcactggca gttcctcctg cctgcacaat ctgacgacca
     4441 tttgcggtga cagtggtgtt tctgcctgtg ccacccgctt ttatccattg cccgccggtg
     4501 ttttcgtcac tgtccggccc cagctcaagc ccggtactga cagtcacgcc atcagctgtt
     4561 ccggatacaa actggttgtc atggtttacc agtgttccgc cattcactgt ttcacccggg
     4621 tgcacaacga tgtcagcagc cagcaccggg agtgacgtga ccgcggcaag agacagtgca
     4681 accgccacac cggtgcgttt cccccgtgcg cgggccagtt cggaggcaac cacgaaagcg
     4741 cccgtaatgt gattccatac cagcctgtag caggtattca gatgtcgttt catcagcatt
     4801 tccttaatca gagtgagggt gaataaaaaa gccggtatcc acagatgtgg ataccggcag
     4861 gtaaagggtt acagtgccgg aggtccggct gacggcagtt tctgacgtgt ggctctgttg
     4921 ttccctgaat ctcatgtctg tgtgtatctg cgcacgttgc taacaggagt tattattagc
     4981 ataacgatcg atattaacga tcggtgaaat aggtatcgat cgttttattc tattatccgg
     5041 ctattgcacg gacaagagag acagtaacga aaacagcgct tttgtgagca gactcagagg
     5101 cagggaggag atcatccaca acaggttcag aagaagatcc agttccatac cgcgcgggca
     5161 acagagacca cggtgtcacg aacagcgcgc agggccgtac ggacaacaga ggcaacacag
     5221 acgctctccg ttgtgtcaaa tatccggtcc accgcaccgg taaactgttc acgggcctgc
     5281 gagcggacag actccgtgcg cagctcgtcc tgcaggcggg tcatcagggg actggcggca
     5341 tggtcgggaa gtgctgtcat gagcgcactg accagcgtat ccagttccca gccggtgcgg
     5401 gccgatacgg ccacaaccgg atgtacgggc cggaacagcc ggaataccgc ctctgttttc
     5461 tcacgaatgt tctgttcctg tgcgggagaa ggctgaatac cggccatatc ccattcatgg
     5521 cagggttccg ttttgtcggc ctgcgtcacc acaaacagta cctgctgatg tccgcatagc
     5581 aggatgtgtc gccagaaata ctcatccaca gacagggcac ggtcatcggc tttaatcagc
     5641 cacagtacca ggtccagttc aggcagaatg tcacggtaca gggcttcata ctctgcatcc
     5701 ctgtcccggc tctcgcccac cccgggcagg tcagtgataa ccatgctgtg accatggcca
     5761 ctcagacgga agcgctgcac ttcccgggtg ccggcgtgaa catcactgac cggtgtgacc
     5821 tccccctgaa acagggcatt acagagtgag gatttaccgg ccccgctttt acccataatg
     5881 ccaatcacgg gttcgtgact ggtgagttta cgcagatgtt ccaggatgtg acgtgaaagc
     5941 gagtaaggca gggaggaaag cggtttttca attgcctcaa tggcatcaga cggattcata
     6001 catgtattcc cggtgaaaaa agacaaaacc ccgcgcaccg gaaaggtggc gggggatcca
     6061 cggagataat atctgtctga aattgttttg aatcgatggg attgttgcgt attaacatga
     6121 aggagatcag gacttatccg ttactgggtg atctccttcc atgaaaatac gcggttattg
     6181 ttaaacagca ggctgattac tccgtaaagg agaaaaatat tcatcttgca gcctgattat
     6241 caggtgtgtt tatgtttact gaggtgagta tttttcagta tattgtcggg gcagtcagtg
     6301 ggagatatta aatttacgtt tagagttatc ttgcttctgc tgtgtatttt tataagagtg
     6361 cgcacgtaat gatatctgtt gataccatgt tgttaagtgt ttcccttccg ggtcatgcgt
     6421 acttgcaaaa tggcatctga atcgatcacg gtttggccaa accgtgatcg aatagagagg
     6481 aatacgcatg taatgattaa atgcttttgt tacatcattc ttccggcatg aatatttatc
     6541 cagttccctt ttttatttgt gtagtgatac tgtcggaata gcgttcagta atgtaaagtg
     6601 tcctgttaaa aatctttaat aaatgtgtca ccctggtcag gaaatatctg ctgagatatt
     6661 attagttggt ggtgttttta ctgagtaata gctggctttt tattcctgaa ctgaaatact
     6721 gtcgctcgtc agggtttaat taaaactgga tgatgatgtg aaaatgaagg ctggtttttg
     6781 tgtttgtcga ggaagtgttg tttttatcca ttctcatcat tggctggctg cgattataca
     6841 gaagtatttg aagaaaaaat ggtgcatttt atctgtgccg gcagggaggg ggattttcct
     6901 gatatgtcag tattaaatgg tttttgttat ttataaaaat gtcatcctgt ccatagaata
     6961 ttgtactgag tactcagcat tcagcgcagg aactgtcatc ttctttattt tattctcctt
     7021 caggagggca gttttctgaa agcgttactc cggggaaact gtttatgttt attcccggca
     7081 ggaaacccgt acctgcaata ccctggatga tgctttttgc tggtacgttg ttgtgtgaaa
     7141 attaatcaac atgtgtgctc cggagagcgc ccgtagacgc acagattcat tctgacattc
     7201 tttagcgaca aaataccgag tggttattat ttaacctgaa aatcgacaga aatttttatc
     7261 ctgtattgat tctgctgcta tttttgctat tagtagcaat agttgcggta ctttatgttc
     7321 tgtgtgttgt gaggtcatta tgccatactt tcagtatcct gatgagtttc ccctgagctc
     7381 gctgcctcca ctcattcgtg atgccgttat tgaagctcag caaatcacgc aggctcccct
     7441 ggggcttgtg gcagcatctg ccctgggcgc tgtttcactt gtctgccaga atctgattga
     7501 tgtctgcaga ctgaatacac tacgggggcc ggtttctctt ttcttactga cgcttgctga
     7561 gtccggagaa cgaaaaaccg cagtggacaa attattaatg gagcccctgt accagcagga
     7621 gatgctgtta tattccagac acaaaaatga actgacgtca tggaaaaata aggaagagat
     7681 tttaaaggcg cagaaaaaag cattgttgtc aaaactgaat aaagaactcc gcaaaggcgc
     7741 ggatgagtca gaaacgctca gacaactgga agcgcttcag aaaaacagag aggaaaaacc
     7801 tgtaagatat aaattcattt ttaatgatgc cacgacagca gcaattaaag atcagctttg
     7861 tggtcagtgg cgttcagtgg gtatcatgtc tgatgaagcc ggtataatat ttgatgggta
     7921 taccctgtct gagctgcctt ttattaacaa aatgtgggat ggctccgtat tgtcggtgga
     7981 cagaaagaat gaacccgaac agatgattga aaatgccaga atgacattat ccctcatggt
     8041 acaacccggt ctttttgacc gatatatgga gcgaaaaggt tctgttgcca gggacagtgg
     8101 ttttcttgcc cgttgtctaa tcagtaaacc tgccaccacg caggggaaac gctttattaa
     8161 cggagctgtg accccgggag gatcattaac ggcatttcat gaacgattaa tggaactggc
     8221 cagaggcagt attgaaaaat ccagtaagga tgaacgttat tgtctgcatt tttctcctga
     8281 agcgcaaaaa atatttattg atcactataa tgttctggag caggatttgt ctccttccgg
     8341 acccttaagc cagtttcggg ggcatgtttc aaaaaaaaca gaaaacattg cgagaattgc
     8401 tgcgttattg cagtatttca gtcagggtga agggaaaatc tctgccgata tcatgacgtc
     8461 tgctgtcgta ataagttcgt ggtacacaga tgaatataaa aaattatttg cgttacctga
     8521 tgaatctgaa ctgcagctgc aggacgcccg ggagctgctc gactggctca ttgaggagtg
     8581 ccgcggagaa tgcccgcccc gtgtcaggaa aaattatatt ctccagtgtg gcccgggccg
     8641 gttcaggaac aggaaaaagc tgaacgtcct gctgaacatt cttgcatctc aactcaggct
     8701 ctctgtcgtg caggaaggaa aaaccaccta tgtcctgctt tccgaaataa acaatatcac
     8761 gctggataaa ctgaatgcgt gctacagtca gaaactgata agatggcatg agcaatcctt
     8821 tggcttataa tgttccctgt tgctgagacg gattattcag tggtggtatc gcagtatgcc
     8881 cgtcaccccc cgcttctgca tgagggtgac ggaggaaaag taagtgtgcg gaggcccggg
     8941 agcgttatga atcaacaggc gtataacagg tactcgtcaa tcatcagttc cagctcgcgc
     9001 ttttcccgtt tgcgcgccac agcccagttt tcaagctctg catgtgattt atgctttaac
     9061 tcttcagtcc agggctgcat ggagtgggca cacatcagat cataccagca cagccatatc
     9121 agcgtgtggt agagatcacg atcgtcgaaa cgccggagca ggctgttcag cctgtttgcc
     9181 agccggtttt cacccagacg atgtagctca ctgagtaaaa cgccgcccag attaaaatgt
     9241 gtaagcagca tgatttcatg ggtctgaatg ctggtcatta acccttcctg aatggcagga
     9301 atgaacggtg gtgttcccgg gttgacgacc tgatagagtc cctggggggc caccgaaatg
     9361 atggagcctt tttccggcag aaaaaagtgt gccagggtca gacggtgata accatgaaga
     9421 tgcggagacc ccattccatg agcgacgata agcctcgtca cgcttgtcat ccatttctga
     9481 tgctggatga gcagcagctc acaccggttt tcaagttttt gcataatatt ctctccttta
     9541 gcgcgcagca aaaggcctcc gggggccgga tcatgccttt gctgcggttg aacggataac
     9601 ggtcaggaat tacggacggg actgtgcaat acgcgcctgc agccaggttt ccacttcact
     9661 tttcagccag cgggaactgc ggcccagttt gatgggggca ggaaaggcac catccttgat
     9721 gagcttgtaa aaccatttat cggtcaggcc ggtcagttga gtgataaatg ccatgtcgac
     9781 catctggtca tccatcagtg aaactggggt attcatgaaa atgttcctcc ggatgttgtt
     9841 aatctcaacc ggcgttggtg atgaaaacaa cgcaccacct tcaccggcat aacaaccggg
     9901 gagaaacgta aaaaataatc gggaatgtga ccgtcaggag ataaacgggg ggattgcggt
     9961 ggctgagccg taccggtatg tcgggtgatt gttgcgaaac gatgcttccg cacgcgcacg
    10021 ggaggacttt cgtgagacaa ggggaaaact ggcgttctga taattgcaga gttgtttttg
    10081 aaggcggttg tggtgcatca gcctgtgacc tgtgggcagg cgacaggtat cactggtggg
    10141 ataagactgt ctgattggta ttataaatat ataagagagc tcaaacacat gagttcatat
    10201 atgtgaaata aatctgtgag tgtgatcagc agaccagtct acccagtagc aacagattgc
    10261 gttgtactca cagggttatc cctgataact ggcgtataac ctgcgtacat aaaacgtacc
    10321 tgcaatgcac ctgaacagag tgcattaagc atccgcctga gcggtaaacg tatccgatct
    10381 ggtgagtgac agtctgagag cattctcagt aatcgccccc ttgtatcctg gaaattggtt
    10441 aagagcgatt agatttggca aggtgtatgc agccagcagc ttaacacctc agctaactgg
    10501 ctggttacct tcaggttgtc gcaatggcat cggatatccg gtgtcggata actaaacgta
    10561 gcgttaaggc gatattgctg agagaccggt taagtggtgg cacgggagta atgagtttgg
    10621 ggagggtacc agaatgccag tcaggtgcgg tatgctggca gtatcacgtt gattgcagca
    10681 taaagtaacg agagacgaat ggaagttaaa accagagcag ttccgtcgtt gttcatcccg
    10741 ggagacgctg gaaaaagtga gttcccacac acgttataaa cttacccctg cggagctgga
    10801 agcctttaac tctgcggtcg atcaccggct ggcagaactg atcatgaaca aactttacga
    10861 tcgcgttccg gcttctgtct ggaaatatgt catctgaacg ttcggtaatc acagcggtat
    10921 tgcgccaggt gttatctgct ctgtgattct tttgctcttc tctggttgct gcaaagctga
    10981 atatgttccc gttgttggaa gccgaggtaa taccgggaac gttaagcggt atagcgacaa
    11041 caaagaattc gaaatcaaaa gggaatggtg tacggaggtg cgggcggtaa tgggccgatt
    11101 ttcgggcatt accgacctct gctgaggttt tctcgtattt accggggggc cggtgcgtta
    11161 ctgtctggca caacaaacgc agcgggtcgc tctgctccgc cgattcctga taatccctcc
    11221 ccagtaacct acggcaacag tacaaataca ctgacgcctg aaacggtaac cagaatcagc
    11281 accagcagta acgtcagtag acagaacaga cgatcggtct tccggtaata accggaagga
    11341 tcgacagctc cacaatccgt aaccagtttt tacattaaaa aaacaacgcc ggtaattcaa
    11401 tcgtcagtcc ggagacgacc gttcgggtta tcacagagtg catgagacat tgcctgctgg
    11461 agataacttc aggggctctt cgcatttttt acagcgtggt tattccgtat gaaccatacg
    11521 gagatttaac catgacctac aaatacaacc ccttctggca acaacgtatc cgtgagacgg
    11581 tgctgcgcgc actggatgtt catccccgcc tgacggcact tcgggttgat ctgcgtctcc
    11641 cggatgtacc ggcagcaacg gacgcagctg tgatatcccg cttcatcaat gccctgaaag
    11701 cacgaatcga cgcttaccag aagcgtaagc gccgtgaagg taaacgcgta catcccacga
    11761 tcctgcatta cgcctgggcc cgggaattcg gggagcttaa aggcaaaaaa cactatcacc
    11821 tcctgctgct ggtcaaccgg gatacctggt gtcgtgccgg cgattaccgc gctccgggat
    11881 cactggccgg gatgattaaa caggcctggt gcagtgccct gggggtggat gccggatgct
    11941 atgccacgct ggcgaatttt ccggcctggc cggtggtgtg gctggaacgc ggtgatgaca
    12001 ccggttttca gcaggtgctg gagcgtgctg actatctggc gaaggagagt accaaagctt
    12061 acggcaccgg tgagcgcaac tttggctgca gtcgtggctg agcccgacag acgacttatc
    12121 tgaccttagt gtgatgatgc gttcacagcg accacactga ttttcccttc tgagactgaa
    12181 ctgaaaaccg ctattcacac ctgaccacgc tgcccgtacc gggacggctg ttgcctgcgt
    12241 gtcgtctggc ggctgaggaa tatgactatg tacgcaaaat cctttctcgc tcttgatggc
    12301 aacgggcgtc tgaccggtgc ccgtactgca cagactgcgc catacgatcg ttatacctgc
    12361 catctttgcg gcagtgctct cagataccat ccgcaatacg acactgagcg tccctggttt
    12421 gaacacaccg acgacgggct gactgaacac gctcagcagt gtccttacgt caggcctgat
    12481 cgcagggaag tgcaactggt taaacgttta cggcaattcg taccggatgc cttacccgta
    12541 gtacgtaaag ccagctggta ctgcagacaa tgtcaccacg attattatgg ggagcggtac
    12601 tgcacacact gccagaccgg gcgcttcagt gaagaggggg gagcagaatg aacggtataa
    12661 aaacggggct ggggatcacg ccgggtgagc acattatcag tgccgactct gcgctcagca
    12721 gaaatatccg gcattgtttc tgcctttcct gtcatggccg gttgattctg cagacagatg
    12781 ctcagggtgc gtggtttgag catgatttac atgccctgag tgagcaacag aaggcggact
    12841 gtgtggtgct gaatccggaa aagtcccacc cttacattga ggatatggcg atgtttttat
    12901 cgccattacc ggtcgtgctg gagtggcatt gtgtgatgtg cgggaggctt tttcccggga
    12961 agaagttctg tgaggcctgt ggtacgggga tttattgcag agcggtatgt acgaagtctg
    13021 tttacagcta ccagccagac ttgtttgagg actgcggaag tgcgagtact taatccgaaa
    13081 tccccgctct gcgcagggcg ttcagaggta ttgccaacct gtattttatc cggtgtttcg
    13141 cacccacccc gttcgtgaca caaaggcgcg acaccggata tccgcagtcc tgcttacctg
    13201 aataaaaccc ggggtattga ttggtagtgt ggtgcggaat cgtaccgtga tctcgcattc
    13261 gccgaattcg cactgtggtg tcaatcaacc acacgggggc gtcgtctcag cctttgcctg
    13321 cctcaggagg gtgaaacctg gggggcattg attaatatcc tgcccgatct gatgaccggt
    13381 acttcagacc tgctaccgat acgccggaat ataacggtca gagaggcata tttcgatgcc
    13441 cctgttctgg agcagttgat gatggatgcc ataggcaatg taagcgcttt gtatcaccgg
    13501 acgaagcacg cagtaccaga agccccccgg ggttcattgg tgacctgaca gcgaagatag
    13561 attcaaagag cgttcagtga tttttcctgc gctcgtcgtg gtgtacgcaa tcaggaatcc
    13621 cgttatctca ttggcatact tgcgatacca caccagcagg acgacgctga aatcacgccg
    13681 acattctcct gtatggcatc aatctcacag gggacattct gagcatgttg acctcaatca
    13741 cagatcacga cagcgtgctc attttactgt cttatatcat tggtgctgac gcaccggccc
    13801 gacaaagggt tttacacgct gattcagtga agaccatttc tctaaaccca tcaaactggg
    13861 gtgaagcaac cgcaagtaca aaagtgaagt gccacagtag atacagcaac gaatcatgga
    13921 ggtcagaagt atgaaacgtg ttgtgat
//
LOCUS       DAZFKF010000118        13892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFKF010000118 DAZFKF010000000
VERSION     DAZFKF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.120"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(607..846)
                     /locus_tag="ACHKA1_004349"
                     /pseudo
     CDS             complement(607..846)
                     /locus_tag="ACHKA1_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(1386..1748)
                     /locus_tag="ACHKA1_004350"
     CDS             complement(1386..1748)
                     /locus_tag="ACHKA1_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681915.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            complement(1739..2275)
                     /gene="yfdR"
                     /locus_tag="ACHKA1_004351"
     CDS             complement(1739..2275)
                     /gene="yfdR"
                     /locus_tag="ACHKA1_004351"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO5681916.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YNVAQHAVLCSKLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNDLSELRKCA"
     gene            complement(2403..3227)
                     /gene="yfdQ"
                     /locus_tag="ACHKA1_004352"
     CDS             complement(2403..3227)
                     /gene="yfdQ"
                     /locus_tag="ACHKA1_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO5681917.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAQFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            complement(3293..3655)
                     /gene="yfdP"
                     /locus_tag="ACHKA1_004353"
     CDS             complement(3293..3655)
                     /gene="yfdP"
                     /locus_tag="ACHKA1_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO5681918.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            complement(4324..4998)
                     /gene="ymfK"
                     /locus_tag="ACHKA1_004354"
     CDS             complement(4324..4998)
                     /gene="ymfK"
                     /locus_tag="ACHKA1_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO5681919.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYTIKA"
     gene            5089..5289
                     /gene="ymfT"
                     /locus_tag="ACHKA1_004355"
     CDS             5089..5289
                     /gene="ymfT"
                     /locus_tag="ACHKA1_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO5681920.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            5333..5884
                     /gene="ymfL"
                     /locus_tag="ACHKA1_004356"
     CDS             5333..5884
                     /gene="ymfL"
                     /locus_tag="ACHKA1_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO5681921.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAAGTHYIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEVIEQIGNYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            6030..6209
                     /locus_tag="ACHKA1_004357"
     CDS             6030..6209
                     /locus_tag="ACHKA1_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5681922.1"
                     /translation="MRELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAH"
     gene            6172..6717
                     /locus_tag="ACHKA1_004358"
     CDS             6172..6717
                     /locus_tag="ACHKA1_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO5681923.1"
                     /translation="MILLSVKDHQHIDSAIPGRYTVQAPYKAGAGIGVLELIKAIYDA
                     PASFLSSAFAHIKVMVGWAGASKDAPVSLITGYANPVQFTTSEIGVSGGGSISPIKEA
                     AIMATVPALTRLNDEDLHKLSYVTTALRALRKVTLSDPQAHQVLVETLINLQAERIRL
                     ADKANFHIHRLLNISGGHRHA"
     gene            6710..6946
                     /locus_tag="ACHKA1_004359"
     CDS             6710..6946
                     /locus_tag="ACHKA1_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681924.1"
                     /translation="MLNPLILNIYRLFQRKKTSIPTVEQWYTTPAGHVLRVSLVDREC
                     QKVICEPLGRNYRVSMPLITFRSGKNMKHLGGAA"
     gene            6943..7761
                     /locus_tag="ACHKA1_004360"
     CDS             6943..7761
                     /locus_tag="ACHKA1_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5681925.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASVDESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARREDPYFKSSYDNVDYSQIPAGFRW"
     gene            7758..8252
                     /gene="yfdN"
                     /locus_tag="ACHKA1_004361"
     CDS             7758..8252
                     /gene="yfdN"
                     /locus_tag="ACHKA1_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5681926.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMEAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            8252..8905
                     /locus_tag="ACHKA1_004362"
     CDS             8252..8905
                     /locus_tag="ACHKA1_004362"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO5681927.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGTAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            8902..9228
                     /locus_tag="ACHKA1_004363"
     CDS             8902..9228
                     /locus_tag="ACHKA1_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO5681928.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRAAHWLH
                     ERGLKA"
     gene            9225..9614
                     /locus_tag="ACHKA1_004364"
     CDS             9225..9614
                     /locus_tag="ACHKA1_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5681929.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            9634..10443
                     /locus_tag="ACHKA1_004365"
     CDS             9634..10443
                     /locus_tag="ACHKA1_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO5681930.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVQELTDTQNLGVGPVNTVRGGPKQGSYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVVSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAHDGSSRPTLSLSALLRQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            10451..11440
                     /locus_tag="ACHKA1_004366"
     CDS             10451..11440
                     /locus_tag="ACHKA1_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5681931.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLVLKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVTFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            11454..12206
                     /gene="ydfT"
                     /locus_tag="ACHKA1_004367"
     CDS             11454..12206
                     /gene="ydfT"
                     /locus_tag="ACHKA1_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5681932.1"
                     /translation="MNLEALPKYYSPKSPKLSDAAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKAGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFAD
                     YSRSAASKARCEPCAGTGFHNVLREVVKYSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGAPVYKICGRCNGNRFSRLPTTLARCHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEVQLRKVTR"
     gene            complement(12478..12567)
                     /locus_tag="ACHKA1_004368"
     CDS             complement(12478..12567)
                     /locus_tag="ACHKA1_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5681933.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLTVIALLIM"
     gene            complement(12622..12834)
                     /gene="cspF"
                     /locus_tag="ACHKA1_004369"
     CDS             complement(12622..12834)
                     /gene="cspF"
                     /locus_tag="ACHKA1_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HFO5681934.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            13135..13350
                     /gene="cspB"
                     /locus_tag="ACHKA1_004370"
     CDS             13135..13350
                     /gene="cspB"
                     /locus_tag="ACHKA1_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFO5681935.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNFRT
                     LFEGQKVTFSVESGAKGPAAANVIITD"
     gene            complement(13714..13884)
                     /gene="ynfU"
                     /locus_tag="ACHKA1_004371"
     CDS             complement(13714..13884)
                     /gene="ynfU"
                     /locus_tag="ACHKA1_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein YnfU"
                     /protein_id="HFO5681936.1"
                     /translation="MSERKDSKSRRNYLVKCSCPNCTQESEHSFSRVQKGALLICPHC
                     NKVFQTNLKAVA"
BASE COUNT         3571 a         3134 c         3526 g         3661 t
ORIGIN      
        1 atatgattca ctgcgtgaag tcattaaaga aaaacaagat aaattaattg ataaaatata
       61 cgctgaaaag attcatactc aaacaatgga gtattggttg agccgagcag gaaaagaaac
      121 agagatttaa tttgttcagt atataaaagc taatttttaa ggagtaattt tcagatgatt
      181 aaagctgatt acattgcgat aggtactcta cgactaactt ttcaacaaga gtaagggggt
      241 aatcaatttt atttgtatca atggtatgac atttcttggc gataatgttt tgatcttatc
      301 gattcatgtc atatggtcat tggtaacgtc cgctcttggt aaagagtgga cgctcagatt
      361 aggtttggca tctaagcata gatgtgtcag ctcacatcta agctaataca tattactcaa
      421 tcactcccgc aaatctgtaa atcttgtgtg atgcccattt atttgggcag gatttaatat
      481 caggatctgg aaggtcaggc ctgtatttct gaccagttct cctgtttacg ctgttccagc
      541 gaagaactgt cgatactgaa acaccacaga aatcggcgac ttgtttagtt gtcattaagt
      601 tgttcattac ttcacctcct gcggcggctc cggtagtggc atccagtggg ttactttcga
      661 tgcctgttct tcctcattgt cagtaactgc ccaccatttg tttctcgacc aatcgtaata
      721 cccttcgaag gtatcgcact cagtccagcc gtaagacttc ccccaacacc aaacatactg
      781 tttatcattc ggcattcgct cactacagct tatccaacca cccggactta ccggagagtt
      841 gccagacagc gcgttctgca gccgttccag cttaacgtat tcctgaaccc tgtttccgtc
      901 gcacacctga agccattgcg cagccttttg cgcatcagtg tgaaaggcac aagtgcgacc
      961 gtcatcaaat tgcatttcgt agaggtcagc aacttgttca aactgcgtgt gtggtgactt
     1021 gtaagtttgg tttacaggtt cggcacaatg aagcatggcg gcgcggcagg cgttccagcc
     1081 ttcatcaaaa ccgactatgc cattatttaa agacggacga gcatctggca ctaccggcac
     1141 tggcttggct atatatagcg gctgaacata ccagcccttt gataaccaac tgtcagcaat
     1201 gtttttactc ctcgttattg ccggaatacc taagccattt tctgaatgca gccatgccac
     1261 cggctcctct tctagcgatg ccagagcaat ttcataagca cggcgctcaa tatcgtctcg
     1321 aacctctagg ctgctgattc gttctttgat ttctttaatc agttctttat tggtaaatgt
     1381 ggtcattatg ctccagcctc cggtgctttt ggcattactg cccagtgagt gatattgacg
     1441 ttttcaaggt ccccgacctg aaatgtccac tgccattctc cggtttcttt ttgtccccag
     1501 gtgtaccaga gagaacgcca gccaatcagc cagccttctc cattagcatc aaataacaga
     1561 acactttcat ttgctggtgg cagttcagct gacactggta ttattttgtt ttccagtgcc
     1621 gcacatttag cttcaagcgc gtcgaattta cgtaccaggt actcagcatt tgtttcgttc
     1681 actttcagat ctcgcggtac acatttcccg cgaagaaacc cttccatttc gaaaacattc
     1741 atgcgcattt gcgtaactcc gataaatcgt taaaacgttc cataaacatc ccgtaggcat
     1801 ggcccggtgc cagtggaatc acgttgaaca tctctgttgc cgggatgcct tccagtacag
     1861 gccagaaaga gccatcatca agcccgagat cgcggcgttc ggttgccagc atgatgagat
     1921 cggcatattt cacgggtgta ctcataaccg ggggtaaccc gtatttctca cggattacgg
     1981 cgtctatttt ttcttccatc cgtttatagt caggaagaag gcgtttcagt ggtgcgggaa
     2041 tgtcctggca atacgcttct gttgcatcat gcattaacgc ttcaaaagca aattcctgcg
     2101 gcaccagttt gctgcaaagc accgcatgtt gggcgacgtt gtagaagtgc gaaagatgac
     2161 cggcaaagcg acagatattt gaaagggaaa ccgcgatatc gttaatatcg atgtcgtctt
     2221 tatttatcct gtcataataa aaatgcttcc cagaaaaagt tttgataaat gacattttgt
     2281 tctccacgta tatgcgctgc accgcgctga attctggtaa aaagaatcct tcaccatccg
     2341 gcgattattg agtaaattac gtttccataa atgcccccgc aggggcattt gcagtaatga
     2401 aatcaggcgg tgaaagtacc aataaaggtt tctactttgc tgtccttgaa tttctcaaca
     2461 agcagatcac gaaattcgtt agccatttcc tcctgcaccg cctccagctg aataatgcgc
     2521 agaaccagta caggacgatc gccagtgata atgctgaggc gtaatttaaa cggacgttct
     2581 ttcagacctt caaacggaac gcatttaaat tcaaatgcca ctggcataat gtctttggtc
     2641 ttcgcttcga cagactccat cagggagcgt ttgccgctga agtcattatc ttcaaaatca
     2701 gcggtctggt ttgcttcaat cgtgatttta cggaccgccg cagccgcttt tgttgcctga
     2761 atggcgtcac cattagcatc aaagcccaca aggtagtcgg cccagtcttc gatccattct
     2821 gccagtgact tctgggagtt acgctcgccg ttaacagaca acagagcaga gaactgtgct
     2881 gtctttttca gtttgagagt ggcagtgtta tctgcgtgac ctggctcatc aatggtaccc
     2941 aggttaagca cactgacggc acgcatatta tcggcatcga taaagcagcg ggtgccttca
     3001 tctgcaagat ctttagaata acgggtaaag tcatcgatgc tggcagtgga aagcgcacca
     3061 cggaaacgga agcgatttaa attaaatttt tccagatcat gaatgcggaa attctcaggc
     3121 aatgccacag catcggcacc aatcttactg ataatttcat taacaccctg agcagaaata
     3181 agggcatgga tttgattaat tgcggttgcg tctaagttct gagacataat aagtcctcac
     3241 tatataaaga tattcagtga tgagataaat aatcagttaa ttaagaacga tattaatgac
     3301 ctgctgcgcg tagttttccg tcaggttcac cggcaagagt cagtaattgt ccctggtctt
     3361 cctgcagaat agtcaggcga ccaccgcgat tgacatacat cggcgtttcg gtggtgtctt
     3421 cttcggaaat tttcccgcgg ttagtcgggc gaacatatga gagtttgtgt ttgattttca
     3481 cacggttctc atcaaatggt tcgatttcca ggttgagtga gaccttacct ttggttttcg
     3541 tgttcatcac accggaagcg acttcactga gaactgcgcc gattttggtt tcaaatacgc
     3601 cgccgtccag ctccccgata aatgcctgca catcagtact gcgttcgcta gccattttgc
     3661 tgctcctcat catatcgacc ctgcaaggcc ggttggtttc tccacaaaac agagaagaac
     3721 acctgcggtg gcagccgccc ggatggattg ggttatgagc ccgtcgtccg gtgatgctct
     3781 tctctgtttt gtaaaaagag cggtaccagc cggaagcaag tgtacaaact ggtaccgcca
     3841 aagcagtggc tgttgtggtg ggggtgtcac tcaggcgtat ggtcaacctg acaatccggt
     3901 gtcctcaacg gggaaagagt aacccagcca tacttaccgc cgcgccattt cgcggatgac
     3961 cacaacgctg agagcactta gccagttacg gcaccacact ttgtcgcggc tccataaatg
     4021 ccctcatcgt tgcaccctgg tctcttccca ggcgtcaaac cggatcgcca cgctggttag
     4081 gcgtcttatc agcatcatca ttgacttgca cattccggct acctggtttg tttgcccgag
     4141 caaggagtgg attgtcccct ttaacgtccc cagaccgcta acgacgcatg tgccatacgc
     4201 cgtgttacaa ccaaattttg tttgaatctt gcctgcctca tgtttctttt ggatacatta
     4261 tgtatctcat gggtacattg tcaagtataa aaaaacctgc cgaagcaggt tcataaatat
     4321 tgattaggcc tttattgtgt atcttcttgg ttttcccgag aaaatcactg taccaattat
     4381 agagcaatta ccgttaatct taatgtaagg ctcaggccag tttgggttta atgctttgag
     4441 ataacgctgt gttccatctt ctatcaatcg cttgaaggtg gtttcgcctg tatcgtgcat
     4501 caatgcaata acgtcgtcac cgtggcaggc agggacttcg ggatcaacaa aaatcatgtc
     4561 tccagggcgg tactcatcaa tcattgaatc accaatcacc cgcaagatat aagtcatttc
     4621 gccacagggt acagggcagg gatacgtttc tgctgtgctc aaatcaacct cagaatagcc
     4681 aacttctttc catgctccgg cctgtaccca tgatatgaca gggactaacg ttatttgttt
     4741 gttagtgatt gaaacatcag gtttttttgt gatgttcgtt gtctggtgtt cttgatcaag
     4801 ccatccgaca ggcaggtcga aacatttttc gatgtgccgt gccatgctgt caccgatatt
     4861 tttagtagcg ccatccccca taaacctgct ggtctgggtt ggctcgcgat caatcatggt
     4921 ggcaaaggat gaatttccgc caacaccatc tctcagtttt ctggcgttag accgccggat
     4981 gtcatggatt gttttcataa cgaaattaaa acctttgtac cgataaggta caagtatctt
     5041 gaaggttcat ttcaatcatg taatatgtat atcggaggta catattgtat gaaagcgtat
     5101 tgggactctt taaccaaaga acagcagggc gagttggccg gaaaagttgg ctcaacacct
     5161 ggctacttac ggctggtttt caatggttat aaaaaagcca gttttgtgct ggcgaaaaaa
     5221 cttgagcaat gcacgtcagg tgcaattacg aaatctgact taagaccgga tatctatccg
     5281 aaagattaac agaacacctt caatttttaa ccacagaacg atgaggctaa ccgtgggtaa
     5341 gtatcactgg aaagtagaaa aacagcctga gtggtacgtg aaagctgtca gaaaaactat
     5401 cgcagcgttg ccgggtggtt acgctgaagc agctgactgg ctggatgtaa cagagaacgc
     5461 attatttaac cgccttcgtg ccgatggcga tcagattttc ccgctgggat gggcaatggt
     5521 tttacagcgc gcggctggca ctcactacat tgcggatgct gtcgcacagt ctgctggtgg
     5581 ggtgttcgta tcgcttcctg aaattgagga agtagagaac gccgatataa accagcgcct
     5641 gctggaagtc atcgaacaga tcgggaatta ctcaaagcag attcgttcgg caatcgaaga
     5701 tggggtcgtg gagccacacg agcagacagc aattaatgat gagttgtatc tgtcaatttc
     5761 gaagctccaa gagcatgcag cactggtcta caaaatcttt tgcgctccag aaaagagtga
     5821 cgcccgcgag tgtgcagctc cgggcgtcgt ggcgttttgt gtctgtggag aaactaacgc
     5881 atgaacagtt taacggcaaa taaccgtttg tcgcaacagc tggtggtcag tgtcgctgaa
     5941 cacctgttgt tacggcatga atgcagatta ccaaatcacc tggctgtaag taaccacaga
     6001 gaactttacc tgactgtggg gggcgagttg tgcgggaact taaccgctgg tttcgtgacg
     6061 gaagagggct ttatgtccat gttattcgtt gggaaccaga aacacagcgc gttatctatc
     6121 ttcgcaaaga ctacccgcat gagtgcttta gtcctttgtg gaaattcagg cgtgattttg
     6181 ttgagtgtga aggaccacca gcacattgat tctgccattc cgggacgtta cactgttcag
     6241 gcaccttata aagcgggtgc cgggattggc gtcctggaat tgatcaaggc gatatatgac
     6301 gcgccagcgt cttttttatc gtccgcattt gctcacatca aagttatggt gggctgggcg
     6361 ggggcatcga aagatgcgcc ggtttccttg atcaccggtt acgccaaccc cgttcagttc
     6421 accaccagcg aaattggcgt ttccggtggt ggaagtattt caccgatcaa ggaggctgcc
     6481 atcatggcta ctgtcccagc cctcactcgt ctgaatgatg aagacttaca taaactcagt
     6541 tatgtaacaa ctgcactacg tgctctgcgc aaggtaactc tttcggatcc gcaggcgcat
     6601 caggttctgg tagaaaccct tattaacttg caggctgaac gtattcgtct ggcggataag
     6661 gctaattttc atattcaccg tctcctgaat atcagcggag ggcatcgtca tgcttaatcc
     6721 gttgatcctc aacatttacc gtttatttca gcgtaaaaaa acatcaattc ctacagttga
     6781 gcagtggtac accacgcctg cagggcatgt tctacgtgtt agcctggttg accgtgaatg
     6841 tcagaaggtg atttgtgaac cgctgggccg taattaccgc gtcagtatgc cgcttataac
     6901 ctttcgctcc ggaaaaaaca tgaagcatct cggaggtgca gcatgagtat ggagctgatg
     6961 gttaaagcga tgaaaattcg agtgggtaat ccattgcgaa aactggttct gatcaagctg
     7021 gctgataatg ccagcgatca gggtgagtgc tggcccagct accagcatat tgctgaccag
     7081 tgcgagatta gcaaacgttc tgtgatgaat catattgcgg ccctttgtga gtccgggctg
     7141 gtaaaaaaag tcacccggaa aggtgaaaaa ggtaactcaa gtaatatcta tctccttcat
     7201 ctggatggtg caggagattc actagggggt agtgcaaata attcactatc tggtgcagca
     7261 aattcaccag gtagtgcagg agttgcacca gggggtagtg caggagattc acccagaacc
     7321 agtcactctt ttgaaccagt caaagaacca gtcaatgaac caatagctgt tggtgcatca
     7381 gttgatgagt ccgtgcgagt tcgttcaaac cgaccggaat attctccgga gtttgagcag
     7441 gcatggctgg catatcccaa acgagctggt ggcaattcaa aatctgcagc cttcaaagcc
     7501 tggaaagccc gtttgaatga gggggtaaac cccgaaacca tgctggaagg tgtgaaacgc
     7561 tatgcgggct gggtatctgc gatgggtaac agcggcacac aatttgtgaa acaggctgtc
     7621 acgttctttg gtccggatcg tcatttcgaa gaatcctggg aagttcctgc ggtatctgca
     7681 gccagacgtg aggacccgta cttcaaatcc agttacgaca acgtggacta cagccagatc
     7741 ccggcaggat tcaggtggtg atcatgagtc ttttgaatga cgttcagaaa ttcattgaag
     7801 cccatccggg gtgtacttcc ggagacattg cggatgcttt tgcaggttac tcacggcagc
     7861 gcgtactgca gtcagcaagc aagttacgtc agagtgggcg tgtggctcac cgttgtgaag
     7921 gggatacacg cagacatttc ccgcgcctga ctgagagagc gcaggagccg gaaccacaac
     7981 cagttcgtga aaccagacct gtgcgcaatt tctatgtcgg cactaacgat ccccgggtga
     8041 ttttgtgcct gacccgccag gctgaagaac tggagtcaag aggcttatac cgtcgtgctg
     8101 caaccgtgtg gatggaggca ttccgtgaaa gccactccca gccagaacga aacaattttc
     8161 tggcgcgtcg tgaacggtgt ttacggaaaa gcagcaagcg ggctgcatca ggtgaagagt
     8221 ggtatctgtc agggaattac gtgggggctt aatgagtaat aaatattgcc aggcgctggt
     8281 ggaactgcgg aacaaaccag cccatgaact gaaggaagtg ggcgatcagt ggcgcacgcc
     8341 ggacaacatt ttctggggaa ttaacaccct gtttggcccg tttgttctgg atctgttcac
     8401 cgacggtgat aacgccaaat gtgccgcgta ttacacggcg gaagacaacg cgctggcgca
     8461 tgactggtca gaacgtcttg cggagcttaa aggtgctgcc tttggtaatc ccccatacag
     8521 ccgcgccagt cagcatgagg ggcaatacat caccggcatg cgttacatca tgaaacatgc
     8581 cagtgccatg cgtgataaag gcgggcgcta tgttttcctg atcaaagctg ccaccagcga
     8641 agtgtggtgg ccggaagatg cagaccatat tgcttttatt cgcgggcgta ttggttttga
     8701 actgcctgcc tggtttatcc cgaaagacga gaagcaggtg ccgacaggcg ctttcttcgc
     8761 tggtgctatt gctgttttcg ataagacctg gaagggaacg gcaatcagct acatcgggcg
     8821 cgatgaactt gaggcatgtg gtgaggcgtt tctggcgcag gttcgccagc aggcggaaaa
     8881 actggtcagg gagatggcgg catgacgaca ttaactcaat gccagcagca ggtgctggat
     8941 atgctgattt cttaccagaa agaacgtggc ttcccgccaa ccaatcagga ggtggcaacc
     9001 atgctgggat accgttcagt gaatgcagcg gtggagcatc ttcgcgcact ggagaaaaaa
     9061 ggcgtcatca cgataaagcg tggcgtggcc cgggggataa cgcttcatac cgcggtgaag
     9121 gacgacgaca gcgaggcggt cgggattatc cgctcactgc ttgccggtga ggaaaacgca
     9181 aggctgcgtg cagcccactg gttacatgag aggggcctga aagcatgaag ctgatcctgc
     9241 ctttcccgcc cagcgtgaac acttactggc gacaccctaa caaaggggcg tttgcaggta
     9301 agagcctgat aagcgcggcg gggcgaaaat tccagagcgc ggcgtgcgca gcaatagttg
     9361 agcagttacg tcgtctgccg aaaccaacgt cggcacctgc ttcagtggag atcgtgttgt
     9421 ttcctccgga taacaggatc cgcgatctgg ataactataa caaggcgctg tttgacgccc
     9481 tgacccacgc aggtgtgtgg gaagacgaca gccaggtgaa aagaatgctg gtggagtggg
     9541 gaccggttat cccggaaggg aaggtcgaga tcactatcag taagtacgag aaaacggcgg
     9601 gtgcagccgc ctgattaaga ggagaaacga agtatgaata atctgatggt cattgatggt
     9661 attgaagttc gtcgtgatgc ttatggacgt tacagcctga acgatctgca tcgtgccgca
     9721 gtagcatctg gtgcaaatgc cagaacgaag gagccgggaa agtttctttc cagccaacag
     9781 actgttgaac ttgttcagga attgaccgat acccaaaatt tgggtgtcgg tccggttaat
     9841 acagttcgtg gtggtcctaa gcaggggagt tatgtctgca aggaactggt gtatgcctat
     9901 gcaatgtgga tcagtccgtc attccatctg aaggtgatcc gtactttcga tatggtagtc
     9961 agtgcaccgg aaaaattatc cgggcaggct gctgacaaga tgcaggcagg tgtgattctg
    10021 ctggacttta tgcgtcggga gttaaacctg tctaactcat ctgtgcttgg ggcctgtcag
    10081 aaactccagg aggctgttgg cttaccgaat ctggcaccgc gctatgccat tgatgctcct
    10141 gctgatgcac acgatggctc aagtcgcccc acgctgtcgc tgagtgcact gctgaggcag
    10201 tatggtatcc gcctgacggc taatcaggca tatcaccaga tggtgaagct ggggatcgtt
    10261 gaacaacgcg aacgatacag ccgtaccgcg attaacaaca tcaaaaaatt ctggtcgctg
    10321 acggcgaaag gctgcatgtt cggcaagaac atcaccagtc ctgcaaatcc gcgcgagacg
    10381 cagccgcatt tcttcgaatc ccgattccct gagctgttaa agctgctcga taccgttcat
    10441 tgaggtgacc gtgagagcac tactgacccc tgaaattgcc ccgcgtatgg ggatcgtatt
    10501 gttcaggcca ggttcagagc tgatgcccct gtttatgcag gggcgtgtcc tgctggagcc
    10561 tgagccggaa cgttattcat ctttcgccag tggtgccgtt ccggcggcat cacaaccgct
    10621 ggcggatgat cctgccgttc gggccgtgtt ccgcaatgag gcagtgatcc gtcgtgctgg
    10681 tggcgtggaa tgtcttgaaa gctggttact tcgtgaaaag ggctgtcagt ggcctcattc
    10741 cgactggcac agcgagaaca tgaccacaat gcggcacgct ccgggagcaa ttcgtctgtg
    10801 ctggcactgc gataaccagc tgcgtgacca gttcacggaa cggctggaat caatggcaac
    10861 ggataactgt gcccgctggg tgttgtctgt cgtgcgtcgg gatctcggtt ttgatgacag
    10921 tcacgttgtg acaatgccgg aactgtgctg gtggctggtt cgtaatgatt tggcggatgc
    10981 cttaccggaa agtgcagccc gtaaggcact gagattaccg aagcctgttg tgccatctgt
    11041 cacccgggaa agtgaccttg tgccttcggt tcctgccacc agcatcatcc aggataaagc
    11101 gaaaaaggtg ctggtgctga aagtggatcc ggagtcgccg gagtctttta tgttacgccc
    11161 caaacgtcgc cgctgggtta atgaaaagta cacgcgctgg gttaagacgc agccgtgcgc
    11221 atgttgtgga aagccagctg atgatcccca ccacctgata ggccacggtc agggtggaat
    11281 gggtacaaaa gcgcatgatc tctttgtgtt gcctttgtgc agaaagcatc acgacgagct
    11341 gcatgcggat accgtgacat ttgaagagaa gtatggctcc caactggagc tgatatttcg
    11401 ttttatcgat cgtgcgctgg caattggcgt actggcgtaa gtggagaacg agcatgaacc
    11461 ttgaagcctt accaaaatat tactccccaa aatctccaaa attgagtgat gccgcaccgg
    11521 cgacaggctc aggtggttta acgattacgg atgtgatggc tgcgcagggg atggtgcagt
    11581 cgaaagcacc gcttgggttt gccttattcc tggcaaaagc tggcgttcag gatcctcagt
    11641 ttgcgattga aggtctgctc aattacgcga tggcactgga taacccgaca ttgaacaaat
    11701 tgagtgaaga aatccggtta cagatcatcc cttaccttgt gagttttgcc tttgctgatt
    11761 attccaggtc tgcggcaagt aaggctcgct gtgagccttg tgctggtact ggatttcata
    11821 atgtattgcg cgaagtggtg aaatactcca gaagcgggga gtctgttatc aaggaagagt
    11881 gggtgaagga actatgtcag cattgtcatg gtaagggaga agtcagcaca gcgtgcagag
    11941 ggtgtaaggg taaaggtatt gtcctggatg aaaaaaggac ccggcttcat ggcgcgcctg
    12001 tttataagat ttgtgggcgt tgcaatggaa accggtttag tcgtttacca acgacactgg
    12061 cgcggtgtca tgtccagaag ctggtaccag acctgactga ttatcagtgg tacaaaggat
    12121 atgcagatgt cattgataaa ctggttacaa agtgctggca ggaagaagca tatgctgagg
    12181 tgcaattaag aaaagtgacg agataaatga ttttcgccga agatggcgac gtaatgcttg
    12241 catttttcaa aaaatatgga taaaattctt tcaacgatgg gctttatata cccgacgtta
    12301 agaaaaagta gaaaacccgc tgatgagcgg gtttttgtgc tttaaatagg gtaatagaga
    12361 tgttgaatct cattccggga ttcatgtttg tttacttatt atttatcggg tgactttgcc
    12421 tgatgtttaa aatgttttct tccagtgcta tgtccctaga tacaatgagt ctgcatatta
    12481 cattattagc agagctatta cggtcaaagt acagcataag cttttaaagc caatcaacca
    12541 gtcatcaaga cagacggggt tattcatgaa aactctccat gtttgattcg atggggcctg
    12601 aaattaaagc tttaatatag ctcatgaaag gtaaacattg gcagctgaag ggccacgcag
    12661 accatttatc cggcaaaatt ccacgcgtaa tccggtggta atttcttctg catcgcggag
    12721 attgagcgct gaaacatgaa gctggacatc gatacgacca tcggatgggg tgataagacc
    12781 cttgccgctt ttgccgtcaa aggttttgac aattcctgtc attttacggg acaaaaaaat
    12841 tccttaatac tgataactgg gcgcactata cacacgttcc tgaagaaagc tatagttttt
    12901 tgatggggtt gaagatggct ggatgtctaa aataaacatt gcttcatatg ttcaactatg
    12961 cgttaatgat tgcgtcggtt tgaagaacag acgatatacg aagtagttta ctaaagcagt
    13021 tctcatttca ggtgttattc acttattcct tctttgagtc tctccaatta agtacgaagt
    13081 cgtttctgtt atacaagcca tttatgccga aaggctcaag ttaaggaatg tagaatgtca
    13141 aataaaatga ctggtttagt aaaatggttt aacgctgata aaggttttgg ctttatttct
    13201 cctgttgatg gtagtaaaga tgtgtttgtg catttttctg cgattcagaa tgataatttt
    13261 cgaaccttat ttgaaggtca aaaggttacc ttctctgtag agagtggtgc taaaggtcct
    13321 gcagcagcaa atgtcataat tactgattaa aattcatagt ttgtctgtat acgataacga
    13381 agaaggctga tgcctgagtg gagatacaga cagagtggtg aatattggat ctctttaata
    13441 aatagtaagg aggtccaata catgaaacaa tggccagcat atttggcaat aacttaatca
    13501 ggaaaagtat gctaaccatt gtggtgaagt gcaggtttgc tgcatgaata gttttacagc
    13561 agaagctaac tgctggcata gcaaaacaaa gtgcgtaagt ggatgactcc cacaaaaagt
    13621 accacaatat taaacccgct caggcgggtt ttttattatc tgctttaaat atgttattaa
    13681 aatataaaaa atacttgtta cgaataaaat caatcaagct acagctttaa gatttgtctg
    13741 gaatactttg ttgcaatgag ggcagatcaa aagggcacct ttttgtactc ttgaaaaact
    13801 gtgttctgac tcttgggtgc agtttgggca ggaacattta acgagataat tacggcgtga
    13861 ttttgagtct ttacgttctg acataggctt tt
//
LOCUS       DAZFKF010000119        13742 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFKF010000119 DAZFKF010000000
VERSION     DAZFKF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.121"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(306..608)
                     /locus_tag="ACHKA1_004372"
     CDS             complement(306..608)
                     /locus_tag="ACHKA1_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO5681937.1"
                     /translation="MNNPNPCMTCGACCAFFRVSFYWAEADDADGTIPVTMTEQISPY
                     HRCMPGTNQKNPRCVALKGEPGVNACCSIYEHRSSTCREFQMSGENGIVNEACNRA"
     gene            complement(773..1636)
                     /gene="potB"
                     /locus_tag="ACHKA1_004373"
     CDS             complement(773..1636)
                     /gene="potB"
                     /locus_tag="ACHKA1_004373"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO5681938.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDD"
     gene            complement(1620..2756)
                     /gene="potA"
                     /locus_tag="ACHKA1_004374"
     CDS             complement(1620..2756)
                     /gene="potA"
                     /locus_tag="ACHKA1_004374"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO5681939.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTAFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            3006..4232
                     /gene="pepT"
                     /locus_tag="ACHKA1_004375"
     CDS             3006..4232
                     /gene="pepT"
                     /locus_tag="ACHKA1_004375"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO5681940.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(4281..5402)
                     /gene="roxA"
                     /locus_tag="ACHKA1_004376"
                     /gene_synonym="ycfD"
     CDS             complement(4281..5402)
                     /gene="roxA"
                     /locus_tag="ACHKA1_004376"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO5681941.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(5478..6938)
                     /gene="phoQ"
                     /locus_tag="ACHKA1_004377"
     CDS             complement(5478..6938)
                     /gene="phoQ"
                     /locus_tag="ACHKA1_004377"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO5681942.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGINISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            complement(6938..7609)
                     /gene="phoP"
                     /locus_tag="ACHKA1_004378"
     CDS             complement(6938..7609)
                     /gene="phoP"
                     /locus_tag="ACHKA1_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO5681943.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(7777..9147)
                     /gene="purB"
                     /locus_tag="ACHKA1_004379"
     CDS             complement(7777..9147)
                     /gene="purB"
                     /locus_tag="ACHKA1_004379"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO5681944.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(9151..9792)
                     /gene="hflD"
                     /locus_tag="ACHKA1_004380"
     CDS             complement(9151..9792)
                     /gene="hflD"
                     /locus_tag="ACHKA1_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO5681945.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(9828..10934)
                     /gene="mnmA"
                     /locus_tag="ACHKA1_004381"
                     /gene_synonym="trmU"
     CDS             complement(9828..10934)
                     /gene="mnmA"
                     /locus_tag="ACHKA1_004381"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO5681946.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRVDVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(10988..11449)
                     /gene="nudJ"
                     /locus_tag="ACHKA1_004382"
     CDS             complement(10988..11449)
                     /gene="nudJ"
                     /locus_tag="ACHKA1_004382"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO5681947.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(11459..12112)
                     /gene="rluE"
                     /locus_tag="ACHKA1_004383"
     CDS             complement(11459..12112)
                     /gene="rluE"
                     /locus_tag="ACHKA1_004383"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO5681948.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            12284..13534
                     /gene="icd"
                     /locus_tag="ACHKA1_004384"
     CDS             12284..13534
                     /gene="icd"
                     /locus_tag="ACHKA1_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO5681949.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(13637..>13742)
                     /locus_tag="ACHKA1_004385"
     CDS             complement(13637..>13742)
                     /locus_tag="ACHKA1_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO5681950.1"
                     /translation="LLKSSQECPPGESEITNKCLHKSCIIKICPYGLK"
BASE COUNT         3436 a         3428 c         3487 g         3391 t
ORIGIN      
        1 ataactgaag catccgacga cgtggtgtct acccaaaaag cgacctgtga atattaggct
       61 ggttaagtgc gtattttcat ggaaggagat cacccaataa ccccgatctc ctttatgtta
      121 atacgcatta agcgggtaaa acaggaagat taagaaaggg agagtttttc gactccccaa
      181 ccaggagaag cagcttataa ctaccagata gttgttatgt catgcgggac ggataatgtc
      241 ataaaaaata tgtcattgcc gtgattatga tcgcgttatt ggagtgggtt tagcccatat
      301 tttgctcatg cgcggttgca cgcttcattc acgatgccgt tttctccgga catctgaaat
      361 tctctgcagg tggatgagcg atgttcatag atgctgcagc aggcattgac tccaggttca
      421 cccttcaggg cgacacagcg aggatttttc tggttagtgc ccggcatgca tcgatggtat
      481 ggagagattt gttctgtcat cgttacgggg atggtgccgt cagcatcatc tgcttcagcc
      541 cagtaaaagg atacgcggaa aaatgcacaa caggcaccac acgtcatgca tggattcgga
      601 ttgttcattt ttatactatt tgttgttttt tcagcgatca gatcaaaggg cgctgaaaaa
      661 aacattctcc aaaattagat tataactatt tgattttatg gatagaggca tgctttttac
      721 gcaaaagtaa aaagcatgcc aaatttgcta ttaacttttt gaatattcag cactaatcat
      781 ctatttcggt tgccttctta ttcagcagac gagaagcgcg ccagtaaacc agcaacatca
      841 ggcccattac gatagtcagc gtaatgctgg tggctgcacc aaacggccag tcacgaatat
      901 taaggaactg gaccttgatg acgttaccga tcagcaggtt tttcgcaccg cccatcaggt
      961 cggacacata gaacaggccc atcgctggca gcatcaccag cagacatccg gcaataatcc
     1021 ccggcatcgt cagtggaata atgatacgga taaaagtctg taacttgctg gcaccgagat
     1081 cgcgcgccgc ctcaagcaac ggcttatcca gtttttcgat actggagtac agcggcatca
     1141 ccataaacgg cagcagaatg taaaccagac cgataatcac cgcactgggc gtgaacatga
     1201 tacggattgg tgtgtcgata acgcccagcc agagcaaaaa ctcgttgagg tagcctttgg
     1261 tgctgaggaa aattttcagc ccgtagatgc gaatcagtga gttggtccag aagggaacaa
     1321 tcagcagaaa cagcagcagc ggacgcacct tgtgtggcag cttcgccaga aaccaggcaa
     1381 acggatagcc cagcaccagg caggcgaggg tggcgatcag cgccatattc agcgagtgca
     1441 atagcacttc aaaatagagc ggatcgagca gacgtgtgta gttatccagc gtaaagacca
     1501 ttttgacgaa actggcgtcg tcgcgggtca aaaagctggt gccaatgatc atcaggttgg
     1561 gcagaaagac aaacaacaca agccaaccga caatagtgac aatcactaca ttctggaact
     1621 tacttgtgtt cttcatcagc cagtacgacc tcccagcttt ctacccaatt aatggccatt
     1681 ttttggtcga gagagtggtc aaagtcagga tcgtcttcat tgaagaattc gctgaccatc
     1741 accatcttgc cattttccag ttcaacaacc gactccagcg tcatgccttt gtagttacgc
     1801 tcgcgaacgt aaccaatcag cccttcagcg tggttgtcgt cgttaatctc ttcaacacgt
     1861 aagtcttccg ggcgcagcag aacatgcagt ttttgccccg gttcaacggc gaagttaacg
     1921 tagatattac attcgcggcc ttcaacgttg gcgcgtacgc gctgctcgtc gagacgttcg
     1981 atgacagtgg cgttaaacat attgatttcg ccaatgaacc cggcaacaaa caggtttttc
     2041 ggctcttcgt agatttcacg cggcgtgccg tcttgctcaa tgcgaccatc gcgcatcacc
     2101 acaatcctgt ccgacatggt gagtgcttct tcctggtcgt gagtcacgaa gacgaatgta
     2161 atgccaagct tacgctgtaa cgctttcagc tcgttctgca tttgcttgcg cagtttgtaa
     2221 tccagcgctg agagcgactc atccagcaac aacagacgag gcttgttaac caccgcgcga
     2281 gcaatggcga cgcgttgctg ttgaccacca gagagctgat gcggtttacg ttgagcgaag
     2341 gtttccagct gcaccatccg cagggcttcc atcacgcggg gcgtaatttc agcagcgggg
     2401 gttttttgca tgcgcaaccc aaaggccaca ttttcgaacg cggtcatgtg ggggaaaagt
     2461 gcgtagcttt ggaaaacagt gttcacatag cggttttccg ccggaacgtg ggtgatgtcc
     2521 tcgttatcca acatgatgcg tccggaatca acagtttcca gacctgcaat caggcgaaga
     2581 acggttgttt taccgcagcc agaagggcca agcagcgtga ggaactcgcc attgttgata
     2641 gtcagatcca gctggggaat gacctcttta ccatcaaagc atttgcgaat tcccgccaat
     2701 tgcaccagcg gtgaaagcga actcggttgt ttattcaatt ttttactctg tcccatgtaa
     2761 acgcaacgga tggcttaccg atgcggggtt tgtggttaac caccttggtg actcttaatg
     2821 agggcggtaa ttctacggca aaccgcttga atcgccaatc tttgttgtga attactggct
     2881 tagctttata ttcattaagg taatgctgat aaatattccc gcttgcaggg gtaaaagtga
     2941 cctgacgcaa tatttgtctt ttcttgcttc ttaataatgt tgtcacaaaa agtgagggtg
     3001 actacatgga taaactactt gagcgatttt tgaactacgt gtctctggat acccaatcaa
     3061 aagcaggggt gagacaggtt cccagcacgg aaggccaatg gaagttattg catctgctga
     3121 aagagcagct cgaagagatg ggccttatca atgtgacctt aagtgagaag ggcactttga
     3181 tggcgacgtt accggctaac gtccctggcg atatcccggc gattggcttt atttctcatg
     3241 tggatacctc accggattgc agcggcaaaa atgtgaatcc gcaaattgtt gaaaactatc
     3301 gcggtggcga tattgcgctg ggtatcggcg atgaagtttt atcaccggtt atgttcccgg
     3361 tgctgcatca gctactgggt cagacgctga ttaccaccga tggtaaaacc ttgttaggtg
     3421 ccgatgacaa agcaggtatt gcagaaatca tgaccgcgct ggcggtattg caacagaaaa
     3481 acattccgca tggtgatatt cgcgtcgcct ttaccccgga tgaagaagtg ggcaaagggg
     3541 cgaaacattt tgatgttgat gccttcgatg cccgctgggc ttacactgtt gatggtggtg
     3601 gcgtaggcga actggagttt gaaaacttca acgccgcgtc ggtcaatatc aaaattgtcg
     3661 gtaacaatgt tcacccgggc acggcgaaag gagtgatggt aaatgcgctg tcgctggcgg
     3721 cacgtattca tgcggaagtt ccggcggatg aaagcccgga aatgacagaa ggctatgaag
     3781 gtttctatca cctggcgagc atgaaaggca ccgttgaacg ggccgatatg cactacatca
     3841 tccgtgattt cgaccgtaaa cagtttgaag cgcgtaaacg taaaatgatg gagatcgcca
     3901 aaaaagtggg caaagggtta catcctgatt gctacattga attggtgatt gaagacagtt
     3961 actacaatat gcgcgagaaa gtggttgagc atccgcatat tctcgatatc gcccagcagg
     4021 cgatgcgtga ctgcgatatt gaaccggaac tgaaaccgat ccgcggcggt accgacggcg
     4081 cgcagttgtc gtttatggga ttaccgtgcc cgaacctgtt cactggcggt tacaactatc
     4141 atggtaagca tgagtttgtg actctggaag gtatggaaaa agcggtgcag gtgatcgtcc
     4201 gtattgccga gttaacggcg caacggaagt aagcgaaaag ggatgcggca tgtgatgccg
     4261 catccggctt aaatccaaac ttacccttcg aagaaccaat acccgctatt gaccagcgcc
     4321 gcgagcatcg cgaggaatga cggatcttcc agcgcatcgc caaaattctc cgcggtcagc
     4381 gcaatgttgc tggcgagtgc atccagtgcc ggacggtgcg gggaatcgat cttctcacca
     4441 ttggcataca cgtcgtcgcc aatgcgcaat acgcgcagac cacccaggcg caccagcaca
     4501 tcaccttgtt tcagcgcatc gtagatttca tccggctgat aaggcggctc tggcggcgcg
     4561 atatccagtt catgacgtga ctgggatata aactccccaa accattgctt aaagtgttcc
     4621 ggctggttga tcaattcgag catcatctca cgcagtttat ccatctcttg cggcagaaca
     4681 tccgcaggat gagcgcgagg tggaacatca ggatcgctgt agtagttgcc gcccagttca
     4741 cgttgcagca cataatcggc aaatccacta atcagttccc gcgtatttgg cgcgcgaaaa
     4801 cccacggaat agttcatcgc attttccagc gcgtagcctt catgcgggaa tcctggcgga
     4861 atataaagaa tatcgccagg ctccagctct tcatcgatga tggcttcgaa cggatcgacc
     4921 tgtaacagat ccgggtgcgg acagtgctgt ttcatttgca gcttttcgcc cactcgccag
     4981 cgacgacgac cggtaccctg aatgataaac acgtcgtact gatcgagatg cgggccgacg
     5041 ccgccgccag gtacagaaaa agagatcatc agatcatcaa tacgccagtc cggtaattca
     5101 cggaacggtc gcatcagcgc ggcggtcggc tcatgccagt ggttcactgc ttgcactaac
     5161 aatgaccagt tagtttcacc gagatgatcg tagctttcga acggaccgtg gctgacctgc
     5221 cattttccat cctggtgact gaccagtcga ctgtcgactt cgctttccat cgccagaccc
     5281 gccaactcgt ctggagagat cgggtcaata aaattattaa agccgcgttt taacaccacc
     5341 gggcgtttct gccagtgacg ttcaagaaaa tcgggccagt taagagtgag ttggtattcc
     5401 atgttaagca tccacaggct ggtatctgca accgattata acggatgctt aacgtaatgc
     5461 gtgaagtatg gacatattta ttcatctttc ggcgtagaat gctggcgacc aaaaatcacc
     5521 tccatccgcg caccgccaag catgctctct ccggcgacga ttttaccctc atattgctcg
     5581 gtgatttcgc gggctaccgc cagccccaca ccttgcccag ggcgtaaagt atcaacccgt
     5641 tgaccacggt cgaaaatgac ctctcgcttg cttaatggaa taccgggtcc atcatcctcg
     5701 accacaatat agagatgctc gtcggtttgc cttgcagaaa tttcgacaaa ctcgaggcaa
     5761 tatttacagg cattatccag cacattgccc atcacctcga caaaatcgtt ctgctcaccg
     5821 acaaagctga tctctggcga aatatcgaga gagatattga tccctttgcg ttgatacact
     5881 ttgttcagcg ccgaggtgag attgtccagc agtggggcga ccggatgcag ctcgcggctg
     5941 agcaatgtcc cgccgcgcat actggcacga tgcaggtagt agccaatttg ctgtgaaatg
     6001 cggctgattt gctccagcat taccggctca gcatcactga cgctcatttt ctcactacgc
     6061 agagaacgca gcgtactttg cagcaccgcc agtggcgttt tcagactatg ggtcaggtcg
     6121 gtgagcgttg tacgatattt gtcgtaacgt tcgcgttcac tttttaacaa tcggttcagg
     6181 tttcgtacca gactggtcag ttctcgcgtt gtggctggat tgagcaattc gcggttatgt
     6241 tcttccagtt cgcggacttc ttttgccagg gcttcgatgg ggcgtaaact ccaccaggcg
     6301 gcgacccaca gcagcgggat cactaacagc agattggctg agagcacata gataaaccag
     6361 ctccagacca tataggaact ttttagctcc accggaatgg tatccaccac cacaatggtt
     6421 aactttggca tccgcgatgt tgccgggtag acgtttaccg ccaccgagtg ggtcatctcc
     6481 gcgtcgtcat catcttcccg cacttcctgc aactgttgct gtatcgaatg atctccactc
     6541 agcaagaggc tggtatcgtt aacatccgct tcaatttcat gaaaaccatt cgatttcagc
     6601 cagtcaggct ggatcatctt catcagccag ggcacgtcac gttgcgccca taaaagctgc
     6661 ccgttctcat cataaattag cgtcatggtg gggctttgct tgtcgatatt ttcgggtaac
     6721 tcgacatgca acttattgtt ttcccacttc gcaagggtat agaacagatt gctctcgcca
     6781 cgtaacagcc gaaacgtagt tttatcgaaa ctgacgctat aaccgatcag cgcgaccatt
     6841 ccgtaggcaa gcgaaagcac cagtactacc gctgccgttg ccaacagaaa acgtacccgc
     6901 agcgagagcg ggaaaaaaag acgcagtaat tttttcatca gcgcaattcg aacagatagc
     6961 cctggccgcg aacggtggta atcacttctt ggggatattg tgcctgaatt tttttgcgca
     7021 gacgtcccat cagtacatca atggtatggc tttcccgcag ctccgcatcc ggatagagtt
     7081 ggagcattaa cgaatctttg ctcaccactt tgccattatt gcgtatcaac gtttccataa
     7141 tggtgtattc gaacgcggtc agtttgatca cttcgtcatt aatagataat tcacggcgag
     7201 agagatcaac ctgaaacggt gggagcgaaa tgacctgtga agccaggccg ctattacgcc
     7261 gcattaatgc ctgcattcgc gccatcacct cttcaatatg aaacggttta gtcacgtaat
     7321 catcagcacc ggcacttaat acttcgactt tgtcctgcca gctttcacgg gcggttaata
     7381 ccagaatcgg cagtgaaaca tcattgctac gccagcggcg aatcagtgac agaccgtcct
     7441 cgtctggcaa tccgagatcg acaatcgcaa tatccggtaa atgttcattg agataataat
     7501 cggcttcttt ggcatcttct gcatcatcga cctgatgacc agcatcctga atctgaactt
     7561 taaggtggtg acgtaacaac gcattgtctt caacaaccag tacgcgcatt tttatttctc
     7621 cctgtcttaa ttattaaaat agtgtaacgc gattatgtgg ttatgggggt aaacattaaa
     7681 taaaccagcg gggaggggag gtaaagtgaa aaaataaaaa gcggataatc ttaataagca
     7741 ggccggacag catcgccatc cggcactgat acgagtttat ttcagctcat caaccatggt
     7801 gatggcgcga ccaatgtagt ttgccggcgt catcgctttc aggcgggctt tctcttcttc
     7861 tggcaacgcc agaccgtcga taaactgctt catgccttcg gcgtcaacgc gcttaccacg
     7921 agtcagctct ttcaatttct cgtacggttt ttcgatgcca tagcgacgca taactgtctg
     7981 gattggttct gccagcactt cccagttgtg atccagctca tccagcagat ggtcacggtt
     8041 cacttccagt ttgctcacgc ctttcagggt ggattgatac gcaatcagcg cataaccgat
     8101 acccacgccg aggttacgca acacggtgga gtcggtcagg tcacgctgcc agcgggaaac
     8161 cggcagtttg cttgccagat gctgtaatac cgcgttggaa aggcccagat tcccttcgga
     8221 gttctcgaag tcgatcgggt taactttatg cggcatggtg gaagaaccaa tctcaccggc
     8281 gatggttttc tgtttgaagt ggttaagggc gatataaccc cagacgtcac ggtcaaagtc
     8341 gatcagaatg gtgttgaagc gcgcaacgca atcaaacagt tcggcaatgt agtcgtgcgg
     8401 ttcaatctgg gtagtgtacg ggttccattg aatacccagc gaggtgacga actcttcgct
     8461 gaactgatgc cagtcaactt ccgggtaagc ggcgatgtgg gcgttatagt taccgaccgc
     8521 gccgttgatt ttgccgagga tctccacctg gttaagctgg cggtactggc gctccatacg
     8581 gtaggcgacg tttgccatct ctttaccgat ggttgacggc gtggctggct gaccgtgggt
     8641 acgagacagc agcgggatat cgcggtactg aacggcgaga tctttaatgc catcaattag
     8701 ttgacgccag tacggcagga tcacttcatc acgcgcggtt ttcagcatta atgcgtggga
     8761 gaggttattg atatcttccg aagtacaggc aaagtggatg aattcagaaa ccgcgtgcag
     8821 ttccgggatc tccgccactt tttctttcag gaaatactca accgctttaa cgtcgtggtt
     8881 agtagtacgc tcgatggttt tgatgcgtgc ggcatcttct tcgctgaaat tggcgacaat
     8941 tgcatcaagg taaccgattg cgtcggcagc aaaagcagga acttccttga tcgctgcgtg
     9001 cgcggccagt ttttgcagcc aacgtacttc aacttgtaca cggaatttca gcaaaccata
     9061 ttcgctgaaa atcccgcgca gcgcgctgac tttatcgccg tagcgtccat cgacagggga
     9121 aacggcggtc agtgaggata attccataga tcacaactcc ggggttaaat gagcaagaat
     9181 ttgttttgcc tgagtggtca ggcgattacg agaaaacatc agttgcagac gtccgccgcc
     9241 gacctggtgc cagagcacgg cggcgcgaat gcctgccagc agggttgcgc gaactttcgc
     9301 ctgcacttgt gggctttgca gtacagcagg ggagccggtg acctgaatgc gcgggccaag
     9361 cgggctaatc acatcaacat agatagcagc catcgcgctc atcagcgttt cggactgtaa
     9421 atcgaagtgt tcgagctggc gttgcaggcc gttgatccgg ttgcccagag tgtcgagcgc
     9481 acctttcgct gaggagagtt tgcgctcaag caccatcagg ctgagtgtgt agcgggttaa
     9541 ttcggcgttt aagccctggc gactgctggc attgagcacg ccgagcaggg tttccagccc
     9601 gacgcgcagg ttggcttcgc taccgccaaa aaccgccagc gtcgagctgg ggttcatatc
     9661 aataatactg ttgagtgaga cgtgtagcgc atcggcatca caatgcccct ggtgagcgag
     9721 ttgttgcacc aggcgtgccg actgacaaat accggccagg gcgagggtga tgtcatagta
     9781 attctttgcc acgttcactg cttccttgtt ttaagtaaag ataataatca gaccggcagc
     9841 ggcagacgct gctcaataat accgccaccg aggcacactt caccgttata gaagacggca
     9901 gactggcccg gcgtcacggc ggcaacgggc tcatcgaaaa tcacttcaat gcgatcatcg
     9961 tccagcgcct tgacggtgca ggggatatcg gtctggcgat agcgggtttt taccgtgcaa
    10021 cgcatagtac cggtgaatgg ttcacgatcg acccagtgca gctgctgggc aatcagaccg
    10081 acagacatca gacgcgggtg ttcatggccc tgcgcaacaa tcagaatgtt gttttcgacg
    10141 tctttgtcca ccacatacca cggttcttcg gtgccttctt tggtgccacc gatacccaga
    10201 cctttacgct gaccgagggt gtgatacatc agcccctggt gctcaccaat ttcatcgccg
    10261 tcaacagtaa tgattttgcc cggttgtgcc gggagataac ggcccaggaa ctcgcggaat
    10321 ttacgttcgc caatgaagca aatgccggta gagtcttttt tcttcgcggt gaccagacca
    10381 agatcttcag caatcttacg cacctgcggt ttttccagtt cgccgaccgg gaacaggctt
    10441 tgcgcaatct gctcatggct gagcgtataa aggaagtagc tctggtcttt attgctgtcc
    10501 agaccacgca gcaggcggct cttgccatcg acatcgaccc gacgaacgta atgaccggta
    10561 gcgatataat cggcacctaa atcttcggcg gcaaattcga ggaaggcttt aaatttgatc
    10621 tctttgttgc agagaatatc cggattcggc gtgcgaccgg ctttatattc ggcaaggaac
    10681 agttcgaaga cgttgtccca gtactcggca gcaaagttaa cggtgtgcag ttcaatgccg
    10741 agcttgtcgc agacagcctg ggcatcagcc agatccgctg ccgctgtgca atattcctca
    10801 ccgtcgtctt cttcccagtt cttcataaac aggccttcga cctgatatcc ctgttgttgc
    10861 aacagccagg cagaaacgga ggaatcaaca ccgccggaca tgccgacgat tacttttttt
    10921 gcggtttcag acattggatc actcacgaca ttgaacttca aggcggcgta ttctatcacg
    10981 cagcctttta gatgacaccc tttgtaaaag gccagttaaa atcgccaatc atctccagcg
    11041 gatagcgttg cccgctttga taacaacgta tactttccgc caccagtggc gaacgaagat
    11101 ttgacgcctg taaaatttct tcggcgctaa cccaacggca gcagtcgata tcgctgtcat
    11161 gaggctgggt cgggcatatt tgctcaagct caatggcaaa aaggaaacgc aaaaacggcg
    11221 ttttatctgg cgcaatccac tgatgcatac gaataaagtg ttgcggctgc gcgctgatgc
    11281 cggtttcttc ccacagctca cgggcggcgg cttccactaa ggtttcatcg gcctccagat
    11341 gcccggcagg ttggttccat aaggctttac cattaatcgt ctcttcaacg actaaaaatt
    11401 tgccttctgc gtgcaccacg caagcaacgg taacgtgcgg tttaaacatt ttgcatcctt
    11461 aatctgtcac ttctcgccat tcgccattag caagattatc caaagagtaa tcacccatcg
    11521 catagcgaat cagtcgcagc gtggggaaac caacatgggc ggtcatgcgg cgcacctggc
    11581 gattacgtcc ttcatataag gtgatcttca gccagctggt gggaatactt ttgcgttcac
    11641 gaattggtgg attccgcggc cataaccacg caggttcgtc aaccagctcc gcgccggcgg
    11701 gcagggtagg gccatcattt aaggttacgc cattgcgcaa ggcttcaagt gcgtcttgtg
    11761 tgggaatgcc ttccacctgc acataataga tttttccggt gcgtttgcct ggctgggtta
    11821 aacgcgcctg tagtgcacca ttattggtca gcaccaataa cccttcgcta tcgcggtcaa
    11881 gacgacctgc tgcataaacg ccctgaaccg ggatgaattc ttttaatgtt ttgcgtccgg
    11941 cttcatcggt gaactgcgga agaacatcgt agggtttatt gaacaggatc acacgcgtgg
    12001 gctggttttc aggtttacgc ctggtagaac gttgcgagct gaatcgctta acctggtgat
    12061 ttctaaaaga agttttttgc atggtatttt cagagattat gaattgccgc attatagcct
    12121 aataacgcgc atctttcatg acggcaaaca atagggtagt attgacaagc caattacaaa
    12181 tcattaacaa aaaattgctc taaagcatcc gtatcgcagg acgcaaacgc atatgcaacg
    12241 tggtggcaga cgagcaaacc agtagcgctc gaaggagagg tgaatggaaa gtaaagtagt
    12301 tgttccggca caaggcaaga agatcaccct gcaaaacggc aaactcaacg ttcctgaaaa
    12361 tccgattatc ccttacattg aaggtgatgg aatcggtgta gacgtaaccc cagccatgct
    12421 gaaagtggtc gacgctgcag tcgagaaagc ctataaaggt gagcgtaaaa tctcctggat
    12481 ggaaatttac accggtgaaa aatccacaca ggtttatggt caggacgtct ggttgcctgc
    12541 tgaaaccctt gatctgattc gtgaatatcg cgttgctatt aaaggcccgc tgaccactcc
    12601 ggttggtggc ggtattcgtt ctctcaacgt tgccctgcgc caggaactgg atctctacat
    12661 ctgcctgcgt ccggtacgtt actatcaggg cactccaagc ccggttaaac accctgaact
    12721 gaccgatatg gttatcttcc gtgaaaactc ggaagacatt tatgcgggta tcgaatggaa
    12781 agctgactct gccgacgccg agaaagtgat taaattcctg cgtgaagaga tgggcgtgaa
    12841 gaaaattcgc ttcccggaac attgcggtat cggtattaag ccgtgttctg aagaaggcac
    12901 caaacgtctg gttcgtgcag cgatcgaata cgcaattgct aacgatcgtg actctgtgac
    12961 cctggtgcac aaaggcaaca tcatgaagtt caccgaaggc gcgtttaaag actggggcta
    13021 ccagctggcg cgtgaagaat ttggcggtga actgatcgac ggcggcccgt ggctgaaagt
    13081 taaaaacccg aataccggca aagagatcgt cattaaagac gtgattgctg atgcattcct
    13141 gcaacagatc ctgctgcgtc cggctgaata tgatgttatc gcctgtatga acctgaacgg
    13201 tgactacatt tctgacgccc tggcagcgca ggttggcggt atcggtatcg cccctggtgc
    13261 aaacatcggt gacgaatgcg ccctgtttga agccacccac ggtactgcgc cgaaatatgc
    13321 cggtcaggac aaagtaaacc caggctctat tatactctct gctgagatga tgttgcgcca
    13381 catgggttgg accgaagccg cagacctgat tgttaaaggt atggaaggcg caatcaatgc
    13441 caagaccgta acctatgact tcgaacgtct gatggaaggc gctaaactgc tgaaatgttc
    13501 agagtttggt gatgcgatca tcaagaacat gtaatctcta catgtgttaa atattgaaac
    13561 gggcgtataa cacgcccgtt gttttattta tgtggatatt attaatagca tatcgagcat
    13621 atttatataa aacctattac ttgagcccat atgggcatat ttttataata caactcttat
    13681 gcaaacattt atttgttatt tcgctttctc ctggaggaca ctcttgactg cttttaagta
    13741 aa
//
LOCUS       DAZFKF010000120        13645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFKF010000120 DAZFKF010000000
VERSION     DAZFKF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.122"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(502..1593)
                     /gene="ychF"
                     /locus_tag="ACHKA1_004386"
     CDS             complement(502..1593)
                     /gene="ychF"
                     /locus_tag="ACHKA1_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO5681951.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1710..2294)
                     /gene="pth"
                     /locus_tag="ACHKA1_004387"
     CDS             complement(1710..2294)
                     /gene="pth"
                     /locus_tag="ACHKA1_004387"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO5681952.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2572..2850
                     /gene="ychH"
                     /locus_tag="ACHKA1_004388"
     CDS             2572..2850
                     /gene="ychH"
                     /locus_tag="ACHKA1_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO5681953.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2905..4584)
                     /gene="dauA"
                     /locus_tag="ACHKA1_004389"
     CDS             complement(2905..4584)
                     /gene="dauA"
                     /locus_tag="ACHKA1_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO5681954.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4709..5656)
                     /gene="prs"
                     /locus_tag="ACHKA1_004390"
     CDS             complement(4709..5656)
                     /gene="prs"
                     /locus_tag="ACHKA1_004390"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO5681955.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5807..6658)
                     /gene="ispE"
                     /locus_tag="ACHKA1_004391"
     CDS             complement(5807..6658)
                     /gene="ispE"
                     /locus_tag="ACHKA1_004391"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO5681956.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVAPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            complement(6658..7281)
                     /gene="lolB"
                     /locus_tag="ACHKA1_004392"
     CDS             complement(6658..7281)
                     /gene="lolB"
                     /locus_tag="ACHKA1_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO5681957.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7495..8751
                     /gene="hemA"
                     /locus_tag="ACHKA1_004393"
     CDS             7495..8751
                     /gene="hemA"
                     /locus_tag="ACHKA1_004393"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO5681958.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8793..9875
                     /gene="prfA"
                     /locus_tag="ACHKA1_004394"
     CDS             8793..9875
                     /gene="prfA"
                     /locus_tag="ACHKA1_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO5681959.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9875..10708
                     /gene="prmC"
                     /locus_tag="ACHKA1_004395"
     CDS             9875..10708
                     /gene="prmC"
                     /locus_tag="ACHKA1_004395"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO5681960.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10705..11097
                     /gene="sirB2"
                     /locus_tag="ACHKA1_004396"
     CDS             10705..11097
                     /gene="sirB2"
                     /locus_tag="ACHKA1_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO5681961.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11101..11910
                     /gene="sirB1"
                     /locus_tag="ACHKA1_004397"
     CDS             11101..11910
                     /gene="sirB1"
                     /locus_tag="ACHKA1_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO5681962.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11946..12800
                     /gene="kdsA"
                     /locus_tag="ACHKA1_004398"
     CDS             11946..12800
                     /gene="kdsA"
                     /locus_tag="ACHKA1_004398"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO5681963.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12949..13056)
                     /gene="ldrA"
                     /locus_tag="ACHKA1_004399"
     CDS             complement(12949..13056)
                     /gene="ldrA"
                     /locus_tag="ACHKA1_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5681964.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13485..13592)
                     /locus_tag="ACHKA1_004400"
     CDS             complement(13485..13592)
                     /locus_tag="ACHKA1_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5681965.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3386 a         3455 c         3515 g         3289 t
ORIGIN      
        1 tcgatgtatc tgcataacaa aaacaatatg cgccattttt gcattactaa taagaaaaag
       61 caaaaaacat cgatattcat ttttgatcaa tgatttttct ggcatatcgt tattatcgtg
      121 ctattggtta tcattttcat cttgccagga tcgcacgtaa tctcattatt cggggacttt
      181 atctgcggga tattaccctt caggtaagtc cttttctcgc cagcatattg gagcgtgatc
      241 aatattgatc agctgtgaag ggggtgatgt gtagtggaac aaaaacttac gttatatcac
      301 tactaatgag atatccgtca tctgaacact gatgtcttta attattctgg tggttcactg
      361 taggcaataa gcacaaaagt gtaggatgtt acaagaatga ttaggactcg gtgaaatgaa
      421 aaatccacgc aattgcgtgg attttatata cttttgcgct cttcatgaga tttagcgaaa
      481 cctcatgaga caacaaatta attagacgtt gaaaaggaag ttcatcacat cgccatcttt
      541 aacgatgtaa tctttacctt ctgcacgcat tttgcctgct tctttcgcgc cttgttcacc
      601 tttgtaagtg atgaaatctt caaacgagat ggtttgtgca cggataaagc ctttttcaaa
      661 atcagtatgg attttgcccg ctgcctgcgg cgcggttgct ccaactggaa tggtccatgc
      721 acgcacttct ttcaccccag cggtgaagta agtttgcagg ttcagcagct tataaccggc
      781 acggatcaca cggttcaggc ccggctcttc cagcccaagc tcctgcataa actcgtcacg
      841 ttcttcgtcg tccagttcgg caatgtctgc ttcaacagcg gcacaaaccg gaaccacaac
      901 agaaccttct ttcgccgcga tttcacgcac ctggtcaaga tatgggttgt tttcaaaacc
      961 gtcttcgttg acgttggcga tgtacattgt tggttttagc gtcaggaagc tcaggtaacg
     1021 aatagccgct ttctcttcag cgcttaaatc cagcgcgcgc agcatacctg cgttttccag
     1081 ctggggcagg catttttcca ggaccgccag ctcagctttc gcgtctttat cgccaccttt
     1141 ggctttcttc tgtacgcgat gaatcgcacg ttcgcaggtg tcgaggtctg ccagcgccag
     1201 ttcggtgttg ataacttcaa tatcgtcagc cgggttaact ttgccggaaa cgtgaatgat
     1261 gttgtcattt tcaaagcagc gaacaacgtg accgatcgct tcggtttcac ggatgttggt
     1321 caggaactgg ttacccagac cttcgccttt cgatgcgcct tttaccagac cggcgatatc
     1381 gacaaattcc atggtcgtgg gaagcgtacg ttgcggtttt acgatttcag ccagttgatc
     1441 caggcgagga tcaggcattg gtacgacgcc tgtgttcggc tcaatggtgc agaatggaaa
     1501 gttggccgct tcaataccgg ctttggtcag cgcgttgaac agggtagatt tcccgacgtt
     1561 gggcaaaccg acgataccgc atttgaatcc catgatttaa atcaccttaa tatcttaata
     1621 atcaacctgt tattgctaac agattgcaga aatggaaata actttgccta ttatacacgg
     1681 cactcgacaa aaatgccgca gacaacgact tattgcgctt taaaggcgtg caatcggttc
     1741 gttgctttgg tcaagccatc tgtaaaccac atttcagtac aacgcgccgc ttcgtcgatg
     1801 gcttcatcaa ttaacttctg ttcactaaca ggcggtttgc ctaacacaaa accgacaact
     1861 ttatttttat cgcccggatg accgattccg atgcgtaaac ggtgaaagtt agggttatta
     1921 cccaatttac tgatgatgtc tttcagtcca ttgtgaccac catggccacc gcccaactta
     1981 aatttggcga cgccaggagg cagatccagt tcgtcgtggg ccaccagaat ttcgtccgga
     2041 ttaatgcgga aaaaactggc catcgccgca acggctttgc cgctgagatt cataaatgta
     2101 gtcgggacta acaggcggac atcttcgcct ccaagggtga ctcgcgaagt ataaccaaag
     2161 aatttagcct cttcgcgcag cggagcgcgc aaacgctctg ccagtaagtc aacgaaccag
     2221 gcaccagcat tatgtcgcgt tgcggcgtat tcagcaccgg ggttcgccag gccgacaatc
     2281 aatttaatcg tcacgttttt ttgtcctgag tgtgtacata actggcgcgt agtttactgg
     2341 ttgcggcccc gcttgacaaa aaactgcgta tcaaatgcag ataacgtaat aattgcctga
     2401 gtggactatt agaaagtcag ggtgttcagg cgtttatttg taaagttttg ttgaaataag
     2461 ggttgtaatt gtgatcacgc ccgcacataa cccacagggt gttgtctata ctttacacat
     2521 aaggaagagg ggtattccct gttacaaccc agaaagttcc ggaggtgaca tatgaaacgc
     2581 aaaaacgctt cgttactcgg taacgtgctc atggggttgg gtctggtggt aatggtggtc
     2641 ggcgtggggt attcaatcct caaccagtta ccacagttta atatgcccca gtatttcgca
     2701 catggtgcag tgctaagtat tttcgtcggt gccattctct ggctggcggg tgcccgtgtt
     2761 ggcgggcatg aacaggtgtg cgaccgttac tggtgggttc gccactatga caaacgttgc
     2821 cgccgtagcg ataatcgccg tcatagctaa caaatactgc agtttgctga ccagtcagtt
     2881 tgcgctgact ggtcaatctc gaacttataa atccgccatc gccgctcgac gattcgggaa
     2941 gaacgcgagg cgtcccggga tcggttgaat gcctgcgcga gccatagtgc gcagtggctg
     3001 gaattccacg ttgcacacgc gcagttcaca tccttcgggc agacgcttca caaaacgctg
     3061 gaacgcatca agaccaccag catcaagtac cggaacggca tcccacttca gaatcacaat
     3121 ccgtttgcct tcaagacgtg actccaggtc cgtgaataag ccttcagcag cagcaaaaaa
     3181 cagcgggcca ataacgcgca gaaccaggac atcgtctgga acatctacga ctaccggtgc
     3241 caggcgagtc atacgtgcga tacgacgcat aaacagcagc gatgccagca cgatccccac
     3301 gctgatggca ataaccatat caaacagcac ggtcagcgac atgcacagca gcatgacaat
     3361 gatgtcatct ttcggtgcat gacgcagcaa gtcgaccact ttatgcgctt cactcatgtt
     3421 ccacgccacc atcaacagca gggctgccat agcggaaagc ggcagccagg agagcagcgg
     3481 tgccagtacc agcagggcaa gaataaccag aatagagtgg atcaccgccg agatagggga
     3541 agttgccccg gcacggacgt tagcggcaga acgcgcgatg gcagctgtag cggtaatacc
     3601 accaaagaac ggagcgatga tattccccag tccctgtcca accagttcgc tattcgcctt
     3661 gtgtttcgtc ccggtcatac catccagcac cacggcgcag agcagagatt cgattgcgcc
     3721 gagcattgcc attgagaatg ccgcaggcag cagtgtgcga atagaatccc aggttaacgt
     3781 gaattctgaa ttaggcagat cccacggcag caccagttgc ggcagcagtt gcggaatacc
     3841 gttaccctga gaaccatcgg ccaggacgta gtggaattgc gaaccgatgg tagcaacatg
     3901 tccgccgagc aggttaacaa tccccatcac cgcgcaacca gccagcaagg ccggaaggtg
     3961 accgggtaaa cgaatgccca gacgcggcca gaaaacaaga atacctagcg tcacaatgcc
     4021 aatggcagca tcacccacat taatggtcgg cagcgccata aataatgcgc cgactttttg
     4081 tagataatgt tccgggacat gggccatttg cagaccgaga aaatctttaa tctgcatggt
     4141 accgatggtg atcccgatac ccgaggtgaa acctaaggtg acggaaaccg gaatatactc
     4201 aatcaggcga ccaaagcgtg ccagacccat cagaatcaaa aagatccccg acagcaaggt
     4261 cgcaaccagc agtcctgcca gcccaaactg ttgcgaaacg ggatagagaa ttaccacaaa
     4321 tgccgcagtc ggaccggaaa cgctaaagcg tgacccaccc gtcagagcaa tgacaatccc
     4381 cgcaacagct gcggtatata aaccgtactg gggtgccaca ccactaccaa tagccaacgc
     4441 catcgccagc gggatagcaa taatcccgac ggttatcccg gcaatcaggt cacgggtaaa
     4501 ccgtgcggca gtatattttt ctttccagca agcgtcgatc agagcgcgga aaggcatcac
     4561 atgtgaggaa aatattttgt tcacaataat gtttcatccg tgagcgcatc atctgtcaac
     4621 taaatggcag gtgaaggagg cataggtcat acaaatggat attacagaca aaaaaacccg
     4681 ccgcagcggg tctttgagcc gggttcgatt agtgttcgaa catggcagag atcgattctt
     4741 cgttgctgat acgacgaatc gcttcggcca gcatacctga cagggtcaga gtacgcacgt
     4801 tcggcagtga tttgatttca tcgctcagcg gaatggtatc gcagacaacg acttcatcaa
     4861 ttacagagtt acgcaggttg ttcgccgcgt tgccagagaa gatcgggtga gtcgcatacg
     4921 caaatacacg tttagcacca cgttctttca gagcttcagc agctttacac agcgtaccgc
     4981 cagtgtcgat catatcatcg accagtacgc agtcacgacc tgcaacgtca ccgatgatat
     5041 gcatcacctg ggaaacgttc gcacgcggac gacgtttgtc gatgattgcc atatcggtat
     5101 cgttcagcag cttagcgata gcgcgggcac gcacaacgcc gccgatgtcc ggagaaacca
     5161 caattgggtt atccagattc agctgcagca tgtcttccag caggatcggg ctaccaaata
     5221 cgttatcgac cggaacgtcg aagaaaccct gaatctgttc agcgtgcaga tccactgtca
     5281 gcacacggtc aacaccgacg ctggagagaa agtctgcaac cactttcgca gtgattggta
     5341 cacgagcgga acggacgcga cggtcctggc gcgcatagcc aaagtagggg ataacagcgg
     5401 tgatacgacc tgcggaagca cgacgcaggg catcaaccat aacgactaat tccatcaggt
     5461 tgtcgttagt aggggcacaa gtggactgga tgatgaaaat atcaccaccg cgtacatttt
     5521 cattaatttg tacgctgact tcgccatcgc taaagcgacc tacagcggcg tcgccgagtg
     5581 aagtgtacag gcggttggca atacgttgtg ctagttccgg ggtggcgtta ccagcaaaaa
     5641 gcttcatatc aggcacgaga agaacctcag gcatgcgtcc attggtggaa agaatctgcc
     5701 gaaaactgtg cgggccaggc atgatccttt ccaggcggtg tattaaagag cgcgatgcaa
     5761 cgtctggaac aaggtgacgt tgtcaccgaa actcagcttg cccggcttaa agcatggctc
     5821 tgtgcaatgg ggaaagatta gcgcctttcg ccacaaagcc attgagccat tccggggctt
     5881 gctctagcac ctggcgggct tcagactctg tatcaaattc agcaaagaca caggcccctg
     5941 tcccagtcag gcgcgacggg gcgtattcta acagccagga aagcaccgca tcaacctcgc
     6001 gaaaacgttt tcttgcgata acctcgcaat cattgctgaa ttcacatttt agcaacgttt
     6061 ctattgacct ttttggcgta ttgcgcggga gttcaggatc tttaaaaatc accggagtcg
     6121 gaatacttac accagggtgc gccaccagat accacttctc tggcggagcc accggcgtta
     6181 gtatttcacc aacgccttcg gcaaacgcgg catgcccccg aacaaagaca ggaacatctg
     6241 cgcccagcgt cagccccatt tccgccagct catccatgct tagcccgcat tgccagagat
     6301 gatttaatgc caccaggacc gtcgcggcat tggatgaacc accgccgaga ccgccgccca
     6361 tcggcaaacg cttgtcaatg ctgatattcg caccgcttcc cgtcggaaga cgcccgctgt
     6421 ctgccgcagt tttcatcaat aaccgcgctg cgcgaacgat caggttatct tcatgttcca
     6481 cgccttcaac gggcgttaac agacgaatat ccccatcgtc acgaagctca atgctgatgg
     6541 tgtcgccgta atcaagaaac tgaaacagcg tttgcagcgt gtggtaacca tccgcacgct
     6601 gaccggtaat gtataaaaac agattaagtt ttgccggaga gggccactgt gtccgcatta
     6661 tttcactatc cagttatcca tttttaactt gatgcgttga ccaccgtcgg tgagttccat
     6721 attggctggc atcgcaggtt gcgttttggt gtcataacca ccataaacaa ccttccagtt
     6781 tttgccattc tggctgtagg taatttcgct caggcggtac tggtcgtcca gtttgtagtc
     6841 ggttgcatca cccggtaaac ctaaaatcca ctggcgcaag ctgttgagcg gaattggcat
     6901 tccagtcaat ttgccaatca tctcttcggc gtcatcggcg gtataacgct gacctttatt
     6961 gtcgactaac tgcacgttac ccggttgagc attcagctcc agttccgtgc tgcccaatgg
     7021 gttagtgagc agcagacggt agcgatcctg gccggtttgc tgccagaaaa agcgggcgta
     7081 cactttttgt tggtcagaaa tataagcgaa cgcgccgcga gtctgatact gattaagatt
     7141 gcgcacgtct tgctgatgct gacgccattg tggcgaatcc gggcttttgc caggaccttt
     7201 gggcgtggta acggaacagg cagtgagcac aagagcagcc agcggtagca ggcggataag
     7261 acgaaaatcg ggcaggggca tagtgatgac aagtccttga gatacgttgc agttataacc
     7321 cttaatgcta gcgttaccgt ccgctatcgt ctatgttcaa gttgtcttaa ttgccagaat
     7381 ctaacggctt tcggcaatta ctccaaaagg gggcgctctc ttttattgat cttacgcatc
     7441 ctgtatgatg caagcagact aaccctatca acgttggtat tatttcccgc agacatgacc
     7501 cttttagcac tcggtatcaa ccataaaacg gcacctgtat cgctgcgaga acgtgtatcg
     7561 ttttcgccgg ataagctcga tcaggcgctt gacagcctgc ttgcgcagcc gatggtgcag
     7621 ggcggcgtgg tgctgtcgac gtgcaaccgc acggaacttt atcttagcgt tgaagagcag
     7681 gataacctgc aagaggcgtt aatccgctgg ctttgcgatt atcacaatct taatgaagaa
     7741 gatctgcgta aaagcctcta ctggcatcag gataacgacg cggttagcca tttaatgcgt
     7801 gttgccagcg gcctggattc actggttctt ggggagccgc agatcctcgg tcaggttaaa
     7861 aaagcgtttg ccgattcgca aaaaggccat atgaaggcca gcgaactgga acgcatgttc
     7921 cagaaatctt tctctgtagc gaaacgcgtt cgcactgaaa cagatatcgg tgccagcgct
     7981 gtgtctgtcg cttttgcggc ttgtacgctg gcgcggcaga tctttgaatc gctctctacg
     8041 gtcacagtgt tgctggtagg cgcgggcgaa accatcgagc tggtagcgcg tcatctgcgc
     8101 gaacataaag tacagaagat gattatcgcc aaccgcactc gcgaacgtgc ccaaattctg
     8161 gcagatgaag tcggcgcgga agtgattgcc ctgagtgata tcgacgaacg tctgcgcgaa
     8221 gccgatatca tcatcagttc caccgccagc ccgttaccga ttatcgggaa aggcatggtg
     8281 gagcgcgcat taaaaagccg tcgcaaccaa ccaatgctgt tggtggatat tgccgttccg
     8341 cgcgatgttg agccggaagt tggcaaactg gcgaatgctt atctttatag cgttgatgat
     8401 ctgcaaagca tcatttcgca caacctggcg cagcgtaaag ccgcagcggt tgaggcggaa
     8461 actattgtcg ctcaggaaac cagcgaattt atggcgtggc tgcgagcaca aagcgccagc
     8521 gaaaccattc gcgagtatcg cagccaggca gagcaagttc gcgatgagtt aaccgccaaa
     8581 gcgttagcgg cccttgagca gggcggcgac gcgcaagcca ttatgcagga tctggcatgg
     8641 aaactgacta accgcttgat ccatgcgcca acgaaatcac ttcaacaggc cgcccgtgac
     8701 ggggataacg aacgcctgaa tattctgcgc gacagcctcg ggctggagta gcagtacatc
     8761 attttctttt tttacagggt gcatttacgc ctatgaagcc ttctatcgtt gccaaactgg
     8821 aagccctgca tgaacgccat gaagaagttc aggcgttgct gggtgacgcg caaactatcg
     8881 ccgaccagga acgttttcgc gcattatcac gcgaatatgc gcagttaagt gatgtttcgc
     8941 gctgttttac cgactggcaa caggttcagg aagatatcga aaccgcacag atgatgctcg
     9001 acgatcctga aatgcgtgag atggcgcagg atgaactgcg cgaagctaaa gaaaaaagcg
     9061 agcaactgga acagcaatta caggttctgt tactgccaaa agatcctgat gacgaacgta
     9121 acgccttcct cgaagtccga gccggaaccg gcggcgacga agcggcgctg ttcgcaggcg
     9181 atctgttccg tatgtacagc cgttacgccg aagcccgccg ctggcgggta gaaatcatga
     9241 gcgccagcga gggtgaacat ggtggttata aagagatcat cgccaaaatt agcggtgatg
     9301 gtgtgtatgg tcgtctgaaa tttgaatccg gcggtcatcg cgtgcagcgt gttcctgcta
     9361 cggaatcgca gggtcgtatt catacttctg cttgtaccgt tgcggtaatg ccagaactac
     9421 ctgacgcaga actgccggac atcaacccag cagatttacg cattgatact ttccgctcgt
     9481 caggggcggg tggtcagcac gttaacacca ccgattcggc aattcgtatt actcacttgc
     9541 cgaccgggat tgttgttgaa tgtcaggacg aacgttcaca acataaaaac aaagctaaag
     9601 cactttctgt tctcggtgct cgcatccacg ctgctgaaat ggcaaaacgc caacaggccg
     9661 aagcgtctac ccgtcgtaac ctgctgggga gtggcgatcg cagcgaccgt aaccgtactt
     9721 acaacttccc gcaggggcgc gttaccgatc accgcatcaa cctgacgctc taccgcctgg
     9781 atgaagtgat ggaaggtaag ctggatatgc tgattgaacc gattatccag gaacatcagg
     9841 ccgaccaact ggcggcgttg tccgagcagg aataatggaa tatcaacact ggttacgtga
     9901 agcaataagc caacttcagg cgagcgaaag cccgcggcgt gatgctgaaa tcctgctgga
     9961 gcatgttacc ggcagagggc gtacttttat tctcgccttt ggtgaaacgc agctgactga
    10021 cgaacaatgt cagcaacttg atgcgctact gacacgtcgt cgcgatggtg aacccattgc
    10081 tcatttaacc ggggtgcgag aattctggtc gttgccgtta tttgtttcgc cagcgacctt
    10141 aattccgcgc ccggatacgg agtgtctggt ggagcaggca ctggcgcggt tgcctgaaca
    10201 accttgccgt attctcgatc tcgggacggg taccggggcg attgcgcttg cgctggctag
    10261 cgagcgcccg gactgcgaaa ttatcgctgt agatcgtatg cctgatgctg tctccctggc
    10321 acaacgtaat gcccagcatc tggcgatcaa aaatatccac attctgcaaa gcgactggtt
    10381 tagcgcgcta gccgggcagc agtttgcgat gattgtcagc aatccgccgt atattgacga
    10441 gcaggaccct catcttcaac aaggcgatgt ccgctttgag ccgctcactg cgctggttgc
    10501 ggcagacagt ggaatggcag acatcgtgca tatcatcgaa cagtcgcgta acgcgctggt
    10561 atccggcggc tttctgcttc tggaacatgg ctggcagcag ggcgaagcgg tgcgacaggc
    10621 atttatcctc gcggggtatc atgacgtcga aacctgccgt gactatggtg ataacgagcg
    10681 cgtaacgctc ggccgctatt atcaatgaca agtttttcta cactgcttag tgttcatctt
    10741 attagtatcg cgctttctgt tgggctatta accttacgtt tctggctacg ttatcagaag
    10801 catccacagg catttgctcg ctggacgcgc attgtgccgc cggttgtcga tacgttgtta
    10861 ttgttaagcg gcattgcgtt gatggctaaa gcgcacatcc tgccattttc cgggcaggca
    10921 cagtggctga ctgaaaagct gtttggagtt atcatttata tcgttttggg ttttattgca
    10981 ctcgattatc gtcgtatgca cagtcagcag gcgcgcatta ttgccttccc gctggcgttg
    11041 gtggtgctgt acatcatcat taaactcgcc accacaaaag taccgttact ggggtaagtc
    11101 atgagatcgt tagctgattt cgaatttaat aaagcgccat tgtgcgaagg catgatcctg
    11161 gcttgcgaag caatccgccg cgattttccc tcgcaagatg tttacgacga actggagcgt
    11221 ctcgttagtc tggcgaagga agaaatcagc cagcttctgc ctttagaaga gcagctggaa
    11281 aaactgatcg cgctgtttta cggcgactgg ggatttaaag cctcacgcgg cgtttatcgt
    11341 ctttccgatg cattatggct ggaccaggtg ttaaagaatc gacagggcag tgcggtatca
    11401 ttaggtgcgg ttttattatg ggtcgcgaat cgtctcgatt tgccgctgct gccggtgatt
    11461 ttccctacgc agctgatatt gcgcattgaa tgtccggatg gcgaaatttg gctgattaat
    11521 ccttttaacg gtgaatcgtt aagcgaacat atgctggacg tatggttaaa gggaaatatc
    11581 agcccgtcgg cggaactgtt ttatgaagac cttgatgaag ctgataacat tgaggtaatc
    11641 cgcaaattgc tggatacact caaagcctcg ttgatggaag aaaatcagat ggagctggcg
    11701 ttacgcacca gcgaagcttt attacaattc aacccggaag atccctatga aattcgcgat
    11761 cgcgggttga tttatgcgca actggattgc gaacacgttg cgttgaacga tttaagttat
    11821 ttcgttgaac agtgtccgga agacccgatc agcgaaatga tccgtgcgca aataaataac
    11881 atcgcgcata aacatattgt gctgcattaa ttaatcgaca ttttactcaa ggttaaggcg
    11941 atcctatgaa acaaaaagtg gttagcattg gcgacatcaa cgtagcaaat gacctgccgt
    12001 tcgtactgtt tggcggtatg aacgtgctgg aatctcgcga tctggcgatg cgcatttgcg
    12061 agcactacgt aaccgtgacc cagaaactgg gtatccctta cgtgttcaaa gcctcttttg
    12121 acaaagccaa ccgctcctcc atccactctt atcgtggacc gggcctggaa gaagggatga
    12181 aaatcttcca ggagttgaag cagacttttg gcgtgaaaat tatcaccgac gttcacgaac
    12241 caagtcaggc acagcccgtt gctgatgtcg tggatgtgat tcagttgccg gcgtttcttg
    12301 ctcgccagac tgacctggtt gaagccatgg cgaaaaccgg tgcggtaatt aacgtcaaga
    12361 aaccacagtt tgtcagcccg ggacagatgg gtaatatcgt tgataaattc aaagaaggcg
    12421 gcaacgaaaa agtgattctt tgcgatcgtg gtgctaactt cggctatgac aacctggttg
    12481 tcgatatgct gggcttcagc attatgaaga aagtgtctgg taactcgccg gtgattttcg
    12541 acgtgaccca cgcattgcaa tgccgcgatc cttttggcgc agcttccggc ggtcgtcgtg
    12601 ctcaggtggc tgagctggca cgagccggta tggcggtagg tctggcgggg ctgtttattg
    12661 aagcgcatcc ggatccggaa catgcgaaat gtgatggtcc atccgcgctg ccgctggcta
    12721 aactggaacc gttcctcaag cagatgaaag cgattgatga tctggtgaaa ggtttcgaag
    12781 aactggatac cagcaagtaa tcttttttgc ttgaaaaata aagtcttagc gttctgcgtt
    12841 aagacttttt tcatgggtgc cggatacaaa aaaggccgca ggctgttatc cctgcggccg
    12901 gtttcgggcg catattgcca tcacggcagc ctgacgcccg ttttcgcctt acttccggtt
    12961 acgccaccag ccgacaatcg ctgcggtaat aattcccgcc aggatcggtg ctgccaggtc
    13021 gtgccagaaa atcatggcaa actgcgcgag cgtcatatag ccgccttgtt gtaatgacaa
    13081 cattttgcgg ctattcttgc attgttgatg gttcaagatt agcccccgtt ctgttgtcag
    13141 gttttacctc tcaacgtgcg ggggttttct ctttccagca accaatgcca ccagggataa
    13201 agcccccgca acattgcgcc tcaccggata acgccggctt ggtgtggata ctacgactca
    13261 attcatcttc acttcatccc tgaaatgttt gcaatgaaga gtgcattccg gtttttcaac
    13321 agctgttaca gtcgttttat gagtgctctg gatgatgctt ccagctcggg ttgccaatat
    13381 ttacttgtgg aagtgataaa gacaaaaatg gccgcaggct gttacccctg cggccggttt
    13441 cgggcgcata ttgccatcac ggcagcctga cgcccgtttt caccttactt ccggttacgc
    13501 caccagccaa caatcgctgc ggtaataatt cccgccagga tcggtgccgc caggtcgtgc
    13561 cagaaagtca tggcaaactg cgcgagcgtc atataaccgc cttgttgtaa tgacaacatt
    13621 ttgcggctat tcttgaagtg tctgg
//
LOCUS       DAZFKF010000121        13532 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFKF010000121 DAZFKF010000000
VERSION     DAZFKF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.123"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            7..837
                     /gene="yjiC"
                     /locus_tag="ACHKA1_004401"
     CDS             7..837
                     /gene="yjiC"
                     /locus_tag="ACHKA1_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO5681966.1"
                     /translation="MSMPLSNTLQSQIITDNHFLHHPKVDSELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESEGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGIANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(978..1751)
                     /gene="uxuR"
                     /locus_tag="ACHKA1_004402"
     CDS             complement(978..1751)
                     /gene="uxuR"
                     /locus_tag="ACHKA1_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO5681967.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1966..3426)
                     /gene="uxuB"
                     /locus_tag="ACHKA1_004403"
     CDS             complement(1966..3426)
                     /gene="uxuB"
                     /locus_tag="ACHKA1_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO5681968.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3507..4691)
                     /gene="uxuA"
                     /locus_tag="ACHKA1_004404"
     CDS             complement(3507..4691)
                     /gene="uxuA"
                     /locus_tag="ACHKA1_004404"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO5681969.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5031..6374
                     /gene="gntP"
                     /locus_tag="ACHKA1_004405"
     CDS             5031..6374
                     /gene="gntP"
                     /locus_tag="ACHKA1_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO5681970.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLIGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6548..7450)
                     /gene="fimH"
                     /locus_tag="ACHKA1_004406"
     CDS             complement(6548..7450)
                     /gene="fimH"
                     /locus_tag="ACHKA1_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO5681971.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPNYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7470..7973)
                     /gene="fimG"
                     /locus_tag="ACHKA1_004407"
     CDS             complement(7470..7973)
                     /gene="fimG"
                     /locus_tag="ACHKA1_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO5681972.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7986..8516)
                     /gene="fimF"
                     /locus_tag="ACHKA1_004408"
     CDS             complement(7986..8516)
                     /gene="fimF"
                     /locus_tag="ACHKA1_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO5681973.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8526..11162)
                     /gene="fimD"
                     /locus_tag="ACHKA1_004409"
     CDS             complement(8526..11162)
                     /gene="fimD"
                     /locus_tag="ACHKA1_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO5681974.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVVCAFAAQTPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTSWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLSQLSAECR"
     gene            complement(11229..11954)
                     /gene="fimC"
                     /locus_tag="ACHKA1_004410"
     CDS             complement(11229..11954)
                     /gene="fimC"
                     /locus_tag="ACHKA1_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO5681975.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11991..12530)
                     /gene="fimI"
                     /locus_tag="ACHKA1_004411"
     CDS             complement(11991..12530)
                     /gene="fimI"
                     /locus_tag="ACHKA1_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5681976.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12595..13149)
                     /gene="fimA"
                     /locus_tag="ACHKA1_004412"
     CDS             complement(12595..13149)
                     /gene="fimA"
                     /locus_tag="ACHKA1_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5681977.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALAETTPTTVNGGTVHFKGEVVNA
                     ACAVDAGSVDQTVQLGQVRTASLKQTGATSSAVGFNIQLNDCDTSVATKAAVAFLGTA
                     IDSAHPKVLALQSSAAGSATNVGVQILDRTGNELTLDGATFSAQTTLNNGTNTIPFQA
                     RYYAIGEATPGAANADATFKVQYQ"
BASE COUNT         3290 a         3481 c         3253 g         3508 t
ORIGIN      
        1 actattatgt ctatgccatt aagcaatacc cttcagagcc aaattattac agataatcac
       61 ttccttcatc atccaaaagt cgacagtgag ttaacacgta aatatgaacg tgcacgcctg
      121 gatacggaaa atatctatct tctgcctctt gctcgtggca ataaccataa ttacgatgga
      181 aaaagcgttg tcgaaattag aaaacttgat attagtaaag agtcctggcc atttaattat
      241 gtgacagaag catgcagaga atccgaaggt attacaacaa caggacgtat gctctatcga
      301 aacttgaaaa tcacctcagc tttagacgaa atatatggtg gcatatgtaa aaaggcgcat
      361 gctgctacag aattagcaga aggtttacgg ctaaatttat ttatgaagag ccctttcgat
      421 cctgttgagg actatacagt tcatgaaata acattaggcc ctggttgcaa tgttccaggt
      481 tacgccggaa ccacgatagg atatatttca acccttccag tttctcaggc aaaaagatgg
      541 accaatgagc agccaagaat tgatatctat attgatcaga taataactgt ctcagggata
      601 gcaaattctt ccggattcgc ccttgccgca ttacttaatg ctaatattga gatgggaaat
      661 gacccaatca ttggtataga agcctatcct ggtaccgcgg agatccatgc aaaaatggga
      721 tataacgtta tccccggtga tgaagatgcg ccactcaaac gcatgactct gcaaccgtca
      781 tctcttccag aactgtttga attaaaaaat ggtgaatgga attatattgg taaatagcca
      841 ctgagaagaa tgagtactaa tattcaggtt atacagatat ttaaacaaca ccctgcaatt
      901 tacaaagtaa tactcattgc gacttcttaa tttaagaaag cgcaaggaac gtttaccctt
      961 gcgcttatta taataagtca ggcgtcgact ttatccagcg gccatgaatc aaacagatag
     1021 ccgtcaaaat aaatatcgtc aacgttcgag aattccagca gacgttgctt aacgttttcc
     1081 agatgctgcc acattgccag cttcgccgct cgggcatctt ttttgattaa cgcggcgagg
     1141 atctgtttgt gatcgcccaa ccactctttg cgatacaggc tgtcatccag atggctgtgc
     1201 aactggatcc acattggatt gttttcccgc cactgccagg actgacggaa taactccacc
     1261 agcatgctgt tatgcgttgc ttcggcaatg gcgagatgga actgcatatc accgctttcg
     1321 ctgctacccg gcgcgctgga agccagttca cgctcttcca gttgcaatgc ctgacgcatt
     1381 ttgacgatat cttcgcgggt agcctgcagg gcggcaaact cggcgatgtt actttccagt
     1441 aactgacgcg cctgtaacag ctcaaaagga cccgcatcat tgcagacgtt ggcatccgga
     1501 ctgtctgtgt tgtggctgcc tgagctatca agaacataga taccggcacc ccggcgcact
     1561 tccaccagcc ctttgatctc cagcatgatc agcgcttcac gtaccaccgt ccgcgtgaca
     1621 tcaagcattt ctgcaatttc acgctccggc ggtagccgtt caccaggatt gtacggcgtc
     1681 tttatgatca gatcgcggat catcgccccg acttcctggt aaggtctttg cgcagaggtg
     1741 gcagatttca tggtgttgtg tccgttaggt gcggttaatc aaatttttgc cggatgcggc
     1801 gtgaacgcct tatccggcct acaacgggca attttacggc ccccgttaat ctcaaactat
     1861 accattagca ctggaagttg gcacgtacag gtgggcaaaa aaacgaaaac tggtcaacca
     1921 aatatcagtt tgattgacca gtaagtctgt agggtaatca gttagttaca gcgcagccac
     1981 acactcgcgc gcaccgcgtt cgcacagctg ctgatatgcc gccgtcaccg cgccaacaaa
     2041 gtcggcattc tgcggcagat catcggcaaa aataccgctc aggcccagca gcgctttcac
     2101 gcggtctgcg ccctgatact gcgcgttaat cttctggaac tcggccagca tcgggtcgac
     2161 cacgtcaatg gcattaccct gctcatccac gccctgggtg taacgcatcc agccagccac
     2221 gcccagcgcc aggtgacgcc agctgccgcc gttttgcagg tgcagacgca ccggatccag
     2281 cagacgctgc ggtaacttct ggctgccgtc catcgcaatc tgccaggtac ggtgacgcag
     2341 agacgggttg ctgaaacgct cgatcagcag cgtcgcatag gcgttcaggt ccgtaccttc
     2401 cggcatcgac agcgttggcg cttgttcctg catcatcagg gcaaaggccg ctttgcgata
     2461 atccgggtta gtcatggtgt cggcaatggt ttcatagccg ccgaggtaac cgaggtacgc
     2521 caggaaagag tggctgccgt tcagcatacg cagtttcatc atttcgaacg gcacaacgtc
     2581 tgcaacgaac tgtgcgccca ctttatccca gtccggacga ccattaacga agttatcttc
     2641 aatcacccac tgacggaacg gttcgcaggc aatggcgcac gggtcgtaaa cacccagctg
     2701 gtcagcaatt tcctgtaagg tttctggcgt cgccgccgga acgatgcggt caaccatggt
     2761 gcacgggaag gtgacgttct cttcaatcca tgccgccagc tgcgggtcac gcgcctgagc
     2821 cagccccaga accgcgacct tcgccacatg accgttttca cgcacgttgt cgcaggacat
     2881 caccgtaaac gctttcaacc ctttctcacg acgcagacgc aacgcttcga cgatgtaacc
     2941 aatcgcggac ttcggcgcag tcgggttttc cagatcgtgc ttgatcagtg ggttattgag
     3001 atccagttga ccgcttgccg catcagcgca gtagcctttt tccgtgaccg ttagagagac
     3061 aatcgccgtt tgcggacgcg ccatcgcgtt gagaatacct tcgcagccgt cgatttccgg
     3121 atgcagcgct tctttcatcg aaccgataat tttcagctcg gtgctttctg cgcctttttc
     3181 cgctacggtg tacagcagtt gctgtttttt caggttttcg atcagcacgc ggtcgttgcc
     3241 tggcatcagg ttaacttcgc agatgcccca gtcgctgtcg gtgctttcca gcagatgatg
     3301 ggtatacagc gcctggtgcg cgcggtgaaa cgccccgcaa ccgagatgca caatgcgtga
     3361 ttccagacga gaatgatccc atgacgggcg ggcaaccggc agattgctgt caacaatagt
     3421 agtcatcgta aactccagaa tccgtcttca gagagtgacg gcaaggaaga aggaaccggc
     3481 acggcgcgca gccatgccgg tggatattaa cggctaaaga aagcgcgctg gatcgccagt
     3541 tcgacaccgc gaacttcggc caggcctttc agacgaccaa ttgcggagta acctgggttg
     3601 gttttcttct tcaggtcgtc cagcatctga tgaccgtggt ccggacgcat cgggatcagg
     3661 tcttctttgc cttccgcttt acgacggtgt tcttcttcaa caatcgcttt caccacttcg
     3721 tacatatcaa cgtcaccgtt caggtgcgcc gcttcgtgga aggttttcgg gttatcttca
     3781 cgcatggtgg agcgcagatg ggtgaagtaa atacgcggac cgaactgctt gatcatatca
     3841 accagatcgt tgtcagcacg cacgccgtag gaaccggtgc acatggtgaa accgttcgcc
     3901 atgctgttta cggtatcaac catccactgc atatcttcaa tggtggaaac aatgcgcggc
     3961 aggccgagga tcgggcgcgg cggatcgtcc gggtgaaccg ccatacgcac gccaacttct
     4021 tcagcaactg gaataatcgc tttcaggaag acggcaaagt tttcgcgcag cttcgcttta
     4081 tcgatatctt tgtacagctc caggtgctta cggaactggt cgagggtata cccttcttcc
     4141 gcgcccggca gaccggcaat gatgttacgc gtcagacgcg ctttgtcttc gtcgctcata
     4201 gtggcgaagc gttcagctgc ctgagcaatt tcttcttcgg tgtaatccgc ttccgcgcct
     4261 ggacgtttca ggatatgcat ttcgaatgca gcgaattcga tctggtcgaa gcgcagagct
     4321 ttggagccgt ctggcagcac gtattcgagg tcagtacggg tccagtcgag caccggcatg
     4381 aagttgtagc acacggtgcg aataccgcac tgcgccaggt tgcgcagagt ctgctgatag
     4441 ttagcgatcc actgctcata gttgccagtg tgggttttga tatcttcgtg aattggtacg
     4501 ctttctacga cagaccacac caggcctgcg tcttcaacga tcgccttgcg tttgaggatc
     4561 tcttctacgg accatacttc gccgttcggg atatggtgca gcgcggtaac cacgccagtt
     4621 gcgcccgcct gacggacatc agctaaagaa accggatcgt ttgggccgta ccagcgccag
     4681 gtctgttcca tgatttttcc tctttcacat agtgggttcc acagcaaaga gacgcggaac
     4741 cctggagttg tttaggtatc cggatgaaca accagaccgc agccgtggcg ttgcatcgga
     4801 tgagatggcg tataagttct accttttggt aagacatgtc aacccgaatt ctaaaattgg
     4861 ttaaccacat cacaagaatt tcactctcat ggttgttgct gcaaattgca gcaaagggta
     4921 attgatggtt attgatcaga ggatgtgaca ttcatcgcaa caatggttga ccaatttaca
     4981 taacatatcg cccaaaataa caacggttca accaggtaag gtaaaacgtt atgcatgtgc
     5041 ttaacattct ctgggtggta ttcggcattg gtctgatgct ggtactgaat ttgaagttca
     5101 aaatcaattc aatggtggct ttgttggtgg cggcgctgtc cgtcgggatg ctggcgggca
     5161 tggatttgat gtcgctgctg cacaccatga aagcgggctt cggcaacacg ctgggggaac
     5221 tggctatcat cgtggtgttc ggtgcggtca tcggtaaatt gatggtcgac tccggcgcgg
     5281 ctcaccagat agcgcatacg ctgctggcgc gtctcggtct gcgctatgta cagctgtcag
     5341 tgattatcat cggcctgatt tttggtctgg cgatgttcta tgaagtggcc tttatcatgt
     5401 tagcgccgct ggttattgtt attgccgccg aagctaaaat tccgttcctg aaactggcga
     5461 tcccggcagt agcagctgcc actaccgcac attcactgtt cccaccgcag ccgggtccgg
     5521 tggcgctggt gaatgcttat ggcgcagata tggggatggt ttatatctat ggcgtactgg
     5581 tgacgatccc aagtgtaatc tgcgcaggtc tgatcctgcc gaagttcctc ggcaatcttg
     5641 agcgcccaac gccatcattc ctgaaagcag atcaaccggt agatatgaac aatctgccct
     5701 ctttcggcgt ttcgattctg gtgccgctga tcccggcgat cattatgatc tctaccacca
     5761 tcgccaatat ctggctggta aaagataccc ctgcctggga agtggttaac tttatcggtt
     5821 cctcgccgat tgcaatgttt attgcgatgg tggttgcatt cgtactcttt ggcaccgcac
     5881 gtggtcatga catgcagtgg gtgatgaacg cttttgaaag tgcggtgaaa agcattgcaa
     5941 tggtgattct gatcatcggt gcgggtggcg tgctgaagca aactatcatc gacaccggca
     6001 ttggcgacac catcggcatg ttgatgtccc acggcaatat ctcgccctac attatggcat
     6061 ggctgatcac tgtgctaatt cgtctggcga cgggtcaggg tgtcgtttcg gcgatgaccg
     6121 ccgccgggat tatcagtgct gcaatccttg atccagcaac tggtcagctg attggcgtga
     6181 atccggcgct gctggtgctg gcgacggctg cgggttccaa caccctcacc cacattaatg
     6241 atgcctcatt ctggctgttc aaaggttact ttgacctgtc ggtaaaagac acgttgaaaa
     6301 cctggggact gctggagctg gtcaactccg tggttgggct gattattgtg ttgattatta
     6361 gcatggtagc gtaaaagaac accagagcct gccaatggca ggctcagttc ggattgtcgg
     6421 caaagtgcgc tttgtttatg tcagatgcga cgctgacgcg tcttatctgg cctacaaagg
     6481 gctaacgtgc aggtttttag cttcaggtaa tattgcgtac ctgcattagc aatgccctgt
     6541 gatttcttta ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca
     6601 cctgccctcc ggtacgtgcg taatttgccg ttaatcccag acttaccgcc gaagttccta
     6661 ctgctcctaa cgataccgtg ttattcgctg gaataatcgt accgttgcgc gtcaactgta
     6721 cgccgacgcc ctgcgctggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat
     6781 ctgcggttgt gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa
     6841 gaggaattgg cactgaacca gggtagttcg gcagagtaac ggtgacatca cgagcagaaa
     6901 catcgcagcc gccagtaggc accaccacat cattattggc gtaaatattc cacacaaact
     6961 ggaaatcatc gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgagc
     7021 cagctttaat cgccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggcc
     7081 acggcttatc cgttctcgaa ttataaacaa cccgcggcgt ttcgctggtg gtcggaaatg
     7141 gatagctact gccactatat tttacggtcc cggaaaaatt agataacacg ccgccataag
     7201 ccgagcctcg ttgcagtgtg acatagtctg taatggtttc cggataatcg ttatggcaaa
     7261 agatttgcgt cgaaagatct acgaccaggt tttgccccac attcacggca ggcgcaaggt
     7321 ttacataaac attagcgctg ccaccaccaa tagggatagc ggtaccattg gcggttttac
     7381 aggcgaatga ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaataa
     7441 ctcgtttcat tacaatcatc tcttcgggtt cagctgtagg tataggtgat gctaatcact
     7501 gcctgaatgg ttccctgagt ggctccgcca tttaccgtta atgctctgac ctgtaacggg
     7561 aagtgcgctg attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat
     7621 gtgttgccac tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctggttttta
     7681 taatatccgg tactgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc
     7741 ggacaattag tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacatc
     7801 agactgaaag aataaagatc gccgagatca accgtggcat tggtggtgga aactgtgcac
     7861 ggtttggcga cgaccttacc gttcaccgtg atggtgacat cggctgcctg tatcgtcgca
     7921 cttgcgagcg ccaatattgc cgccaataaa tacccacgtt tgcgccattt catgagcatc
     7981 tccagttact gatattcaag agtgaaggta gccgtggcat tgatatgccc cgcagtgaca
     8041 ggcacctgtg tcgccattag ccgggcgtaa aaattcaacg tatttggttt acccggcgtc
     8101 agggtcgtcc acgaaagcgc ggacgatgga gcattaaggg gtatttgatt ttgctgctca
     8161 ttcagaagct gtattcccag tcccgaagcc gctgacaccg tattttcaag tgcaagcagg
     8221 ttggcattgt ggctatctgc aacaccggta aacccaactt ttacggcaga aacggcatta
     8281 ccacagggtg acagcaaaat acgaaatgga acgacaggag tcgtcgcgcc aatgttgtta
     8341 aattgcttcg ccgcgttttc catcaaatca acagtaaaat tggttgattc agcggccaca
     8401 ctacagccgt tatctctgac atagccgcgg atagtaatcg tgctatccgc agccaaagcg
     8461 tgactcaccg ccagccacaa aaaagcgcac agaagataaa aaggtttgtt tctcatcacg
     8521 cccccttaac gacattcagc tgatagctgg cttaataact gctgctgact ctctggtggc
     8581 agttgataat tggcgacaca gtgagcattt tcctcttctc cccatttcac ctgaactttt
     8641 cccgctaaag gcattccgct gaggtaaacc tgaccattat ccgcaacaat gccgctactc
     8701 tggctactct ctgatgtcac catcgcccca aacggcagcg gcttattatt gtgggttagc
     8761 gtcatgagca gttttatccc aacgcgcgct ttaaactctg ctcgcacgat cgccccacga
     8821 gtgggaacaa cgttagcgac cgcgttatct aaatcgacgt tatcagccag ggtattggta
     8881 tccagcgcca ctctgttttc ccgatattcg gtggcataag gcagcacggc ataaccgcgc
     8941 cagtcggtac gcacccccgt ctggttttcg acttttgcat cttttgcgcc aggcgcttta
     9001 acaagcacca ccgtatcgtt taacggctgc cccagcgtta cgccattggc atgagccagt
     9061 accccaccgc tgactccgta atagagctgc ttaatatcat cgctatggct gtaaccgata
     9121 ttggcattgc cgtaaccacc gcgataattc agcgtggcgt agcctgtgct tccgctatta
     9181 ccatcgcctc ccccggcata gccggtttgc acgctatagc tgaggttgtt gtcttccagc
     9241 aacgtaccgt atacaccagc cagattggtc atccgaccgt tgagatcgtg tgacatgctg
     9301 tagctggcac tggcatgtcg ccactgagat ttgctgtcag aacgcagcca gtggctgaaa
     9361 ggaatattga cgttaagcgc taacatctga tcccgtcctt tttgccaggc gtttttcgtc
     9421 aggctatagc tgagcgtcca gttgatatct tcgaacgcag tatttaatcc agcctggaat
     9481 tgctcatcga cattactcgt tccccaataa gtttgatggc taccactcaa atacagtgtt
     9541 gatgtgcgcc cgagttgctg agtaacggtg agctgtaatt tcccgcgttt gttataagcg
     9601 aggttgtaat agtcggtgaa tttcggctta acctgaataa ctccatcctg tgtttcgatg
     9661 ttgtagccat tcattcgact gtatgttgta tcagcgaaat taaaatatcc gctggtcgaa
     9721 taacggtaac ccactaactg aatattcgtg cctgactcat tgagcgattt gttatagaga
     9781 aaacgcaccg attgtccgtc atgctgactg tcatcgggaa gtgtggaatt agcctgcgtc
     9841 atatccacag acagagcgcc cagtgccccc atgtttttcc cgataccgaa attaaaagca
     9901 cgataacgat ccgccagttg cgttccacca tatattgtcc agccagccgg aaggccgtgg
     9961 agtaatgtac tctggaaaaa gcggggtttt tcctgctgcg catttccact acggtattct
    10021 cctgccgtaa tggaataacg agtatgccct tcacgttgca aaagcgggac tgacgaatag
    10081 ggtacggtaa aaatctgcgt gctgccgtca gcctctttga tcgttacctg caagtcacca
    10141 ctattacctg cggcatagat atcgttgatg gtaaaaggcc ccggtggcac cgtactatta
    10201 taaatgtcat acccattttg tttaatagtg acctgtgcag taccacgagc aataccgtgg
    10261 atcaccgggg caaatcctct ttggctatcg ggtaacatat tgtcatctga ggccaattgt
    10321 gcgccgcgaa agttaatacc atcgaaaata tcgccctggg tataaccatc acccagcgtc
    10381 agccgggaac gtaacggtat tatgtctcgc tcaagccagg tattgatatg ctgccattta
    10441 tttttgctac ctgatgaact gtcgctactg ttataactcc agctggtatt gtcgcgtaaa
    10501 cgccacgcac caatatttaa cccactctgt aggtttaaat atgcataatg gctgttaccc
    10561 ccaatccgat tctgtacact atttccgctg aaattataat tgagcaatcc ggcattaata
    10621 ccgggatccc ataactcagg aggaatataa ccacgcgcgc gattactcat aaatgcctga
    10681 gggatcgtca ggttcagtcg ctgctgacca acatctaaat gcgcagtagc gtcctggacc
    10741 attgtggtta atggcacgca ggcatcatcc gccagcagat tcataccgga gacagaagcc
    10801 gtattcagcc ccatactggc aagttgcgcg cgtgtcaggc agggaacaat cccttgttcg
    10861 ctgtcgcccg tattaaatgt gacatcacgc gttgccatat aaccattatt caaatagata
    10921 tcgacacgat acgtccctgg cggtaattct tgcccatttt caaaacgcga taaatcagcc
    10981 acagcctggg gatcatccgc taaaaagcgc ggattaaaat agagttcggc agatgacaaa
    11041 ggtgtctgtg cggcaaaagc acagacgaca acgagccgga caaaaaaacc agccaaacga
    11101 tgcttacgaa tatgcaagca ttgtgtgttt cgctggtaaa gtcttaaatt cagatatgac
    11161 attattcctc cggtttaaaa tcacccggca gtcaattctt tttattcagg cgaaaaattc
    11221 cccctgcgtt attccattac gcccgtcatt tttggggtaa gtgcaccata atcatttatt
    11281 gttcggtaag taatattgct tcctgcatca gaaggcaatt taaccgtgct ttcgcccatt
    11341 ggaggcacca atgcattttc aagaacccgg gttccggcat tcaactctgt taccgtcagg
    11401 taatagggtg tcgggttaat cagcgtcaga gaattcgcgc tacgacgaaa tcttaatttt
    11461 tctgcggcct gatcgggtgg caacgctaat ttagccggac gatagtacag tttaatgcgg
    11521 ctgataattg cgagctgtag cgtattctca gtcaattttg atttatccat tgacggaatc
    11581 gctttaacgt tcatccagaa taaactttcc cggtcctgtg gcaattggtt atttgttgca
    11641 tcaagaatac gcaaggtatt ctcttttttt cccttcatcg caaacagagg aggcgtcacg
    11701 ataaaacgac catcctttac accatcggca ttttccaccc atgattgaat taaataggta
    11761 ctattttcat cattatttgt cacggcaagt tgcacttgtt tttgccctgc cggataaatt
    11821 acgcgagtcg cacctaaggc cactcccgct tcagcgcgtc cggcaaccat cattgccatg
    11881 aacatcagga tacctgccag caagcagaat gttatttcct gcgatttcct tacattgacg
    11941 tttttattac tcactgtcct gttcctgtta tcacattatc tgctgaacaa ttactgatag
    12001 gttaaagaga accaggcctg tgcattggcg atgccgccag taacccgacg cccggtagca
    12061 cgatatttgg cgatgaaatg tagcgaagta gagcctgagt aaagccgttt ccagtttgct
    12121 ggaggacgat taatcggtac gaggtttcct tcatcatcaa acaacgctac gccaatattg
    12181 gtggctatcc ctggcccctc tcccacggaa agcacatccg gatttttacc atccgcgaca
    12241 ccgtgaaacg ccacacctac acgttcactc accaccgtgc tacattcccg taaatgaata
    12301 acaaaaggca ccggtgcgct atcttcccct accgcatgaa accggttact gctgatttgc
    12361 cccatattga ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc cgcaataatg
    12421 acgccctgaa attgcatatt tccgccgggc agcgtggtat tccatttatt tccggccaga
    12481 gcaaacattg gcagcaacga cgctaataga aataatctct tccttttcat cgttattttt
    12541 atcgcacaag ggtgggcatc cctgcccgta atgacgtccc tgaacctggg taggttattg
    12601 atactgaacc ttgaaggtcg catccgcatt agcagcaccc ggggttgcct cgccgattgc
    12661 ataataacgc gcctggaacg gaatggtgtt ggtaccgtta ttcaaggttg tttgtgcact
    12721 aaatgtcgca ccgtccagcg tcagctcatt tcctgttctg tccagtatct gcacaccaac
    12781 atttgttgcg ctacccgcag ctgaactctg cagagccagt actttaggat gagcactgtc
    12841 aatcgcagtc cccaagaagg caacagcggc ttttgtggca acactggtat cgcaatcatt
    12901 cagctgaata ttaaaaccga cagcagagct ggttgctcca gtctgcttca gactagcggt
    12961 acgaacctgg cccaactgaa cggtttgatc aacagagcct gcatcaactg cgcaagcggc
    13021 gttaacaact tcccctttaa agtgaacggt cccaccattt accgtcgtgg gtgtagtttc
    13081 agccagagcc gctgcggaac tgagggacag agccgacaga acaacgattg ccagagtttt
    13141 aattttcatg ctgctttcct ttcaaaaaac tatttctaaa tcgacatggg cagtcgttct
    13201 gtacactttg ttttgtcaac gagtttgact gccaacactg cacagttttc ccccaaaaga
    13261 tgaaacattt ggggccattt tgactcatag aggaaagcat tgcgcacaaa ctttttcagt
    13321 taatttgttg gcttaatatt ctattgttat ctttatttat agatttttat attgcatgag
    13381 gtggtttttg gagagaagaa tgaggaagag gcgtcgagcc acagaaacgt tagctttaca
    13441 tatagcggag gtgatgtgaa attaatttac aagagaaata atttacatat caaacagtta
    13501 gatgcttttt gtcgtttttt aatattttta tg
//
LOCUS       DAZFKF010000122        13284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFKF010000122 DAZFKF010000000
VERSION     DAZFKF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.124"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..581
                     /locus_tag="ACHKA1_004413"
     CDS             <1..581
                     /locus_tag="ACHKA1_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO5681978.1"
                     /translation="IKNTFLDYSNFYMAYMAEVNLYKVIAPYINLFRADLSFSKLDLI
                     NFEHADLSRVNLNKATLQNINLIDSKLFFTRLTNTFLEMVICTGSNMANVNFNNANLS
                     NCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFFE
                     EDCTSHTGMSQTEDNINAVAMKITADIMQDAD"
     gene            complement(735..2384)
                     /locus_tag="ACHKA1_004414"
     CDS             complement(735..2384)
                     /locus_tag="ACHKA1_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO5681979.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2535..3884)
                     /gene="ghxP"
                     /locus_tag="ACHKA1_004415"
                     /gene_synonym="yjcD"
     CDS             complement(2535..3884)
                     /gene="ghxP"
                     /locus_tag="ACHKA1_004415"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO5681980.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4430..4894)
                     /gene="soxR"
                     /locus_tag="ACHKA1_004416"
     CDS             complement(4430..4894)
                     /gene="soxR"
                     /locus_tag="ACHKA1_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO5681981.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSVRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4980..5303
                     /gene="soxS"
                     /locus_tag="ACHKA1_004417"
     CDS             4980..5303
                     /gene="soxS"
                     /locus_tag="ACHKA1_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO5681982.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5306..6892)
                     /gene="pdeC"
                     /locus_tag="ACHKA1_004418"
     CDS             complement(5306..6892)
                     /gene="pdeC"
                     /locus_tag="ACHKA1_004418"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO5681983.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCSPAHQKRMLNIIRGYLYINELIYAHDN
                     HFLCSSLIAPVNGYTIAPADYKRDPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7321..7602
                     /locus_tag="ACHKA1_004419"
     CDS             7321..7602
                     /locus_tag="ACHKA1_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO5681984.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(7701..8237)
                     /gene="ssb1"
                     /locus_tag="ACHKA1_004420"
     CDS             complement(7701..8237)
                     /gene="ssb1"
                     /locus_tag="ACHKA1_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO5681985.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8492..11314
                     /gene="uvrA"
                     /locus_tag="ACHKA1_004421"
     CDS             8492..11314
                     /gene="uvrA"
                     /locus_tag="ACHKA1_004421"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO5681986.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11349..11705)
                     /locus_tag="ACHKA1_004422"
     CDS             complement(11349..11705)
                     /locus_tag="ACHKA1_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO5681987.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11709..12125)
                     /locus_tag="ACHKA1_004423"
     CDS             complement(11709..12125)
                     /locus_tag="ACHKA1_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO5681988.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12236..12949)
                     /gene="aphA"
                     /locus_tag="ACHKA1_004424"
     CDS             complement(12236..12949)
                     /gene="aphA"
                     /locus_tag="ACHKA1_004424"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO5681989.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3458 a         3451 c         3238 g         3137 t
ORIGIN      
        1 atattaaaaa taccttcctt gattattcaa atttttatat ggcatacatg gctgaggtaa
       61 atctttataa agtaatagcg ccatatatta atttatttag agccgacctt agcttctcta
      121 aacttgattt aattaacttt gaacatgctg atctgtctcg tgtcaacctg aataaagcaa
      181 ccctccagaa tataaactta attgatagca aactcttttt tacgcggtta acaaatacgt
      241 tcctcgaaat ggttatatgt accggctcta atatggctaa tgttaatttt aataatgcca
      301 atttaagcaa ttgccatttc aactgttcta ttttaacaaa agcctgtatg tttaataccc
      361 gtctctatcg ggttaatttc gatgaggcta gcgtccaggg aatgggcatt tccattctcc
      421 gtggggagga aaatatcccc attgatagtg ataccctggt aacactacag aaattctttg
      481 aagaagattg tacctctcat actggcatgt cacaaactga agataatatt aatgcagtcg
      541 ctatgaagat tactgcagat attatgcaag atgcagattg aggttaagcc gaagaacagc
      601 caatgaattg actttacaag agagataact tatttgtttt agaatcatga atgttagccc
      661 gagctaacct gttaaaggtg caaaagattg cacctttttc agattacctc gggcctggtt
      721 acgtcgcttt cggcttactg attttcctca atcaacaacg cttcaagcaa atcgagatcg
      781 tgcagtaatt tttgcagcgt ttcgttactg atctcccgcg tggcgcgtag gtggtacagt
      841 tcagcacgtt cagaacgcaa tgccgccaga cggaagcgac gctcaaggtt ctcttcctgc
      901 acggaacttt caacgtcgtt acgtccatcg gcgcgacgac gcaggttacc aatgacgcga
      961 gaactgacct ccgtaagcag ctggttatcg atattctctt cggtatccgc cgccagacgc
     1021 tcctccattt tctggatcgc cacaatcgcc acttctgccg ttgccgctcg cgcaatacgt
     1081 tcttctttca attgttgcga atggtccgcg acttcaatgt gttgtagcag aataggcaac
     1141 atcaccacgc cgacaaacag cgagaagaga atgacaccag ccgccagaaa caccagctca
     1201 tagcgcgccg ggaagacgtt accatccggc aagagcagcg ggatagagag cacaccggcc
     1261 aaagtgatcg ccccacgcac cccggcgaaa gacgcgatca ggatttctcg tgtggtccac
     1321 gaaccaaact ccatcggctt cttcttcagg aagcggttgc tgaacttttt catcgtccac
     1381 agccagccga aacggaccag catcagcgcc gcatatatca gaataatatc ggtaaacagc
     1441 atccagattt cgacgttagg gtcgatttct gctgccatca gcgacgtctc cagaataccc
     1501 ggcagctgca gacctaacag caggaatacc atgccgttaa agacaaattc cagcatcgcc
     1561 caggtgctgt ttgcacgcag gcgcattgcc agcggcgcac ggcgcatcac accggagcgg
     1621 gtgatggtca tcccggcggc aaccgcagcg aggatgccgg aaacgccaat atgttccgca
     1681 atcagataag aagcgaatgg cagcaggaac aacaggacga tctgcgtcgc gggttcatca
     1741 ccgccccagc ggctgaggaa tcgcagcgag cgaccgtaca gccagctaac cacaaaaccg
     1801 gcgagaatac cgccaatggc tactttcata aattcgaccg tcgcaccgcc gacggtgaag
     1861 atcatcgtcc ccatcgccac tgctacggca aacttcaacg acaccaggcc ggaagcgtca
     1921 ttcatcaacg cttcgccctg caaaatgccc atgatttttt tcgggatgcg cccttcgcca
     1981 acaatcccgg agagcgccac agcatcggtc ggagaaagta ccgccgccag cgcaaaggcg
     2041 gggatcagcg gaatgcccgg caccacccag taaataagga agccaatgcc gaccacggtg
     2101 accaccacca gcgccagtgc gaggccgaaa atctctcgac catgttcgag aaattcacgg
     2161 gtcggcgttt tccagccatc agcgaacagc aacggcggga taaacaagac taaaaagagt
     2221 tcaggatcaa actccacatg caaaccaaac gtcggccacg ccagtagcgc accgatggcg
     2281 atttgcataa gcggaagcgg gatctgaaag ggcatgacac gagtgaccac cccggacagc
     2341 gagaccacga gggtcattat cagtatggtg aagaagattt ccatgcgttc cctgtttgcg
     2401 atgtttttta tgatctgaga aagacgttat gcctgaattc tcattttttc agaatatcgc
     2461 aaaagctcac acaatgtgcg gagaaaataa aaacgggcag ccattgccac ccgttttcag
     2521 aatccgatga aagattagat agcccagcca cccgcataga aggtcaccag cgccacggcg
     2581 ataacgaccg taccgatgtt caacttgcgc cattcgccgg aaaccagacg accaatcacc
     2641 agggtcgcga agccgatcat gatgcctgtt acgatgttac aggtcagcac gatgaatacc
     2701 gccgtaacca gacccgccat cgcatcaaca aaatcagcaa agtcgatttt cgccacgttg
     2761 ctcagcatta gcaggccaac gtacatcagc gccggagccg ttgcataccc cggaacgagg
     2821 taagaaagcg gagagaggaa cagaatcagc aggaacagca cgccaacggt gatagccgtc
     2881 aacccggttt taccgcccgc cgccgtaccc gccgcagact cgatgtatac cgctgccgga
     2941 gctgcaccca ccaggccaga gaaaacgctg ctcatggagt cagtggtcag tgctttcccg
     3001 ccgtcgatga tctgcccatc tttatccagc aggttcgcct ggccggcgac ggcacggata
     3061 gttccggtgg catcaaatac tgccgtcatc accagcgcca gaacgcttgg caggactaca
     3121 ggattcagcg cgcccataat gtccaggctg ccaatcagtg aattgccgtt ttcatcgctc
     3181 aatgaaggca tggcgaaaac gccggagaaa tggacgttag gatcgaagat caaaccgaca
     3241 attgagatac cgataatggt cagcagaatg ccaccaggga ctttcagttt ttccaggccg
     3301 atgatcaccg ccagacctac cagtgacata atcaccggga aggtcgcgaa atcacccagc
     3361 gcaacgggca gaccatcaag cgggttttta atcaccagac cgacaccgtt agcggcaatg
     3421 agcagcagga acagaccgat accgatcccc gtgccgtgcg ccacaccgtg cggcaagttg
     3481 cgcaaaatcc agctacggat acccgtggca gaaattaccg taaacagcac acccatcagg
     3541 aacacggcac ccagcgcgac aggtacgcta atatgttgcc ccagcaccag gctgaatgcg
     3601 gtaaacgccg tcagggagat ggcgcaacca atcgccaacg gcagattagc ccacagaccc
     3661 atcacgatag aaccgagtcc ggcaaccaga caggtcgcaa cgaaaactgc cgcaggcggg
     3721 aagcccgctt tacccaacat gcctggaacg acgataaccg agtagaccat cgccagaaac
     3781 gttgttaacc cggcaaccac ttcctgacgg acagtgcttc cacgttgtga aattttaaac
     3841 caggcgtcga gtgaaccgcc ggtacgcgct gatggcgtag acataagaga acatcccctg
     3901 agagtttaat tttcgtcaag atgcgtggcg gacaatcccc tgccagcgaa acgttatctt
     3961 cctgctccag gtttgcgaca atgtgctgcg tcgcaaaaaa gcaaacgttt agctccgcgc
     4021 atacaaaacg ggtggtaaaa aaagcaaacg attatccggc gatcatagcg gctttttcaa
     4081 ccgaagtttg ctgctttttt ctcttttatt gtcttgacga tgaaaaacca ggcaattgat
     4141 gcgcaaacgt tatcgtcgat gcgcaagatt gcagcgttat cgagccgtat accgttctgg
     4201 attgcgataa aaaatcgtct gagatgacaa aaatattgcg gctaaaaagt atacttttac
     4261 actggcgaag aaacgccggt gacatacaat taaagcatca acaccaaccg gaacctccac
     4321 cacgtgctcg aatgaggtgt gttgacgtcg ggggaaaccc tcctgtgtac cagcgggata
     4381 gagagaaaga caaagaccgg aaaacaaact aaagcgccct tgtggcgctt tagttttgtt
     4441 catcttccag taagcgtgcg ccggtacctt cttctcctaa gcggtcgccc gggttacgca
     4501 acgggcaatc actgcgcgaa aggcagccac aaccaataca tccatccagt tcgtcacgca
     4561 gcgccactaa ggtatgaatg cgccgatcca actcttctcg ccattgggac gaaagctgtt
     4621 tccactcttt cgcacttaac gtatgccctt cgggcaacac gccaaacgct tcaccaatgg
     4681 tcgccagcgg aatgccaata cgctgagcaa ttttgataat tgcgacatat cgcaacacat
     4741 cacgtttata tcggcgctga ttgccgctat tacggacact ggtaatcaac cctttgcttt
     4801 catagaaatg cagcgccgat accgccacac cgctgcgttt cgccacttcg ccgggggtta
     4861 gcagcgcttt aatgcggggt aatttctttt ccataaatcg ctttacctca agttaacttg
     4921 aggaattata ctccccaaca gatgaattaa cgaactgaac actgaaaaga ggcagattta
     4981 tgtcccatca gaaaattatt caggatctta tcgcatggat tgacgagcat attgaccagc
     5041 cgcttaacat tgatgtcgtc gcaaaaaaat caggctattc aaagtggtac ttacagcgga
     5101 tgttccgcac ggtgacgcat caaacgcttg gcgattacat tcgccaacgc cgcctgttac
     5161 tggccgccgt tgagttgcgc accaccgagc gtccgatttt tgatatcgca atggacctgg
     5221 gttatgtctc gcagcagacc ttctcccgcg ttttccgtcg gcagtttgat cgcactccca
     5281 gcgattatcg ccaccgcctg tagttttatt gcccgcgcgt taactcccgc gcgggtaatt
     5341 gctccatcca ttgcataaac acctgcggcg gcatcgcctt cgcaaaaaac catccctggc
     5401 aatagcgcac gccgcgtttg cgcagccagt taacctgctc ctccgtttcg acgccttcag
     5461 cgatcgtttt taaccccagg ctgtgcgcca gctcgatgat atgttccgca atcaaatgac
     5521 tggttttatg ggtggtcagc gtctcaacaa acgatttgtc gattttcaaa atatcgacat
     5581 tcaatgattt aaggttatgc aagttagagt agccaatacc aaaatcatca attgccactt
     5641 cgtaaccggc ctggcggaaa gccagaataa tcggcgtcat tttgtcgaca tcaagaaatg
     5701 catgttcagt cacttcaaat ttaatttgct gtggacgcac cgcgtattgc tctgttttct
     5761 gattgattcg cgctatcaac cgtgacgtat gaaaatcgga ggccgacaga ttaatagaaa
     5821 catagcgatc agcatgtgtt gcaaggtacg cgcccagatc gcggaagaca ttatcaataa
     5881 cataatcagt tacctgttct atcatcccct ccttttctgc cagcggaata aactctgccg
     5941 gattcattac ttgcccctgc tcaccaggcc aacgtaacaa cgcttcagcg ccgatacatt
     6001 tttctgtttt gatatcgatt attggctggt aataaagaca aagttgatgt ttttctaggg
     6061 cgcgttgcaa tttgcgtttg ggagacaaat agttttgtcg aatacgtagc cagagtaata
     6121 gcagaacaag actccccaaa ataccggcgg gtaacgcaaa aataagatga ttataaaaat
     6181 gggctataag acgttgatat gaagtcgcaa caatggctgc aattgggcgt tttgtcgaat
     6241 aaactgtcgc atataaatag ccatttcttt gtacggttaa atccttcaaa tgaatcagcg
     6301 gagaaaacgt tgctgccgag gcctcgttgc ttaacgagaa aaaggttttc gtcaccgtat
     6361 catacacacc ccattgcaat gtcgggtcat cagacatcac ttcactccag aagagagggt
     6421 tgataaccgc cacataattt ccccgctgca tataggtcat tttatagcca gagaaaaaag
     6481 gcgtatcgcg gtaataatag atagaaacgt tagggtcacg cttataatcg gccggtgcaa
     6541 tcgtatagcc gtttacaggc gctatcagcg atgagcataa aaaatggtta tcatgggcat
     6601 agatcaattc attaatataa agatagccac gaataatatt taacattcgc ttttgatggg
     6661 ctggagagca aacttgcccc tgatagcgtt ctgctgcatc gctcactaaa tctgcctggc
     6721 gaatgaccag ctcggattta tccagtgcca gttgagcaaa ggttcggagc tgattattca
     6781 cttctgattt tgcccaaagg aaggcaatcc atagcgaaag aatgatggga aagagaacta
     6841 aaaagataat gcccggcaac gccagtaatt ggtgtcgtgc acgatgactc attgctatca
     6901 atttcctgcc tgtttatacc cggccattgc aaggtgaaac cctggctacc gacgtcttta
     6961 gataaatctt acggtataaa tagcactttt gcctgatttg aaattgatac ctttttaatc
     7021 gtcacctgga tgaaaataat tcattgatat tcaaaattaa ttttctacag gaatacagag
     7081 gcggcgggaa gatataccct taatgccact gaaaaaccac ataatctgta cagcaataaa
     7141 agtcacgacc taatctggct aacaaacaat caattgtgat atagttcaca aaattaatga
     7201 aacaaacaga gtgtttcatt tttgtgttct gcaagccccg cgatagccag agtgtaccac
     7261 gcctcccgtg aacaacgccg cgctgtccag ggcatcggct cttttcagag gaattgtttg
     7321 atggctaccc tcaccactgg cgtggttctt cttcgctggc aacttcttag tgccgtaatg
     7381 atgtttctgg ccagcacgct caacatccgt tttcgtcggt ctgattatgt cgggcttgca
     7441 gtgatcagca gcggtctggg cgtggtttcc gcctgctggt tcgcgatggg gttgcttggc
     7501 atcacaatgg cggatatcac cgccatctgg cacaacatcg agtcggtgat gatagaagag
     7561 atgaatcaga caccgccaca atggccaatg attttgacct gatacgtaga agcctccata
     7621 acggaggctt cttttttatg gctggcagat gctttaatca tccaccttaa aacaatataa
     7681 cctattgttt taatgacaaa tcagaacgga atgtcatcat caaagtccat cggcggctcg
     7741 ttagacggcg ctgccggagc ggactgctgc gggcgagact gcgcgccgcc gctgaactga
     7801 ttgccaccct gcggctgctg aggctgaccc caaccgccct gcggctgacc accaccgata
     7861 ttgccacctg caggagcgcc accaccctga cgaccaccca gcatctgcat agtgccgcca
     7921 acgttcacca caacttctgt ggtgtagcga tcctgaccag attgatcggt ccatttacgg
     7981 gtacgcagct gaccttcgat ataaacctga gaacctttac gcagatattc gctggccact
     8041 tctgccagtt tgccgaacag cacaacgcgg tgccattcag tctgctcttt catctcgccg
     8101 gtcgctttat cacgccagga ttcggaagta gccagcgtaa tgttggcaac tgcgccacca
     8161 tttggcatgt agcgtacttc cgggtcctga cccagattac caacgagaat aaccttgttt
     8221 acgcctctgc tggccatgtt cgtgtctcct gaaaaaaaat cgttctgaat aagtgtaaac
     8281 gtgcgattgt accattacca atagcgcttt tactatgttg tgacctcggt tccgggaaac
     8341 aaacctggcc agacattgtt acacaacact ccgggtaatg cattccaata ctgtatattc
     8401 attcaggtca atttgtgtca taattaaccg tttgtgatcg ccggtagcac catgccaccg
     8461 ggcaaaaaag cgtttaatcc gggaaaggtg aatggataag atcgaagttc ggggcgcccg
     8521 cacccataat ctcaaaaaca tcaacctcgt tatcccccgc gacaagctca ttgtcgtgac
     8581 cgggctttcg ggttctggca aatcctcgct cgctttcgac accttatatg ccgaagggca
     8641 gcgccgttac gttgaatccc tttccgccta cgcgcggcag tttctgtcac tgatggaaaa
     8701 gccggacgtc gatcatattg aggggctttc tcctgccatc tcaattgagc agaaatcgac
     8761 gtctcataac ccgcgttcta cggtggggac aatcaccgaa atccacgact atttgcgttt
     8821 gttgttcgcc cgcgtcggcg aaccgcgctg tccggaccac gacgtcccgc tggcggcgca
     8881 aaccgtcagc cagatggtgg ataacgtgct gtcgcagccg gaaggcaagc gtctgatgct
     8941 gctcgcgcca atcattaaag agcgcaaagg cgaacacacc aaaacgctgg agaacctggc
     9001 aagccagggt tacatccgtg ctcgtattga tggcgaagtc tgcgatcttt ccgatccgcc
     9061 gaaactggaa ctgcaaaaga aacataccat tgaagtggtg gttgatcgct tcaaggtgcg
     9121 tgacgatctt acccaacgtc ttgccgagtc gtttgaaacc gcgctggagc tttccggtgg
     9181 taccgcggta gtggcggata tggacgaccc gaaagcggaa gagctgctgt tctccgccaa
     9241 cttcgcctgc ccaatttgcg gctacagtat gcgcgagctg gagccgcgac tgttttcgtt
     9301 taacaacccg gcaggtgcct gcccgacctg tgacggcctt ggtgtacaac aatatttcga
     9361 tcctgaccgc gtgatccaaa accccgagct atcgctggct ggcggtgcga tccgtggctg
     9421 ggatcgccgc aacttctatt atttccagat gctgaaatcg ctggcagatc actataagtt
     9481 cgacgtcgaa gcgccgtggg gcagcctgag cgcgaacgtg cataaagtgg tgttgtacgg
     9541 ttctggcaaa gaaaacattg aattcaaata catgaacgat cgtggcgata cctccattcg
     9601 tcgtcatccg ttcgaaggcg tgctgcacaa tatggagcgc cgttataaag agacggagtc
     9661 cagcgcggta cgcgaagaat tagccaagtt tatcagtaat cgtccgtgtg ccagctgcga
     9721 agggacgcgt ctgcgtcggg aagcgcgcca cgtgtatgtc gagaatacgc cgctgcccgc
     9781 catctccgac atgagcattg gtcatgcgat ggaattcttc aacaatctca aactcgcagg
     9841 tcagcgggcg aagattgcgg aaaaaattct taaagagatc ggcgatcgtt tgaaattcct
     9901 cgttaacgtc ggcctgaatt acctgacact ttcccgctcg gcagaaacgc tttctggcgg
     9961 tgaagcacag cgtattcgtc tggcgagcca gattggtgcg ggcctggttg gtgttatgta
    10021 tgtactggac gagccgtcta tcggcctgca ccagcgcgat aacgagcgcc tgttgggtac
    10081 gcttatccat ctgcgcgatc tcggtaatac cgtgattgtg gtggagcacg acgaagacgc
    10141 gattcgcgcc gctgaccatg tgatcgacat tggcccgggc gcaggtgtac acggcggtga
    10201 agtggtcgcg gaaggtccgc tggaagcgat tatggcggtg ccggagtcgt tgaccgggca
    10261 gtacatgagc ggtaaacgca agattgaagt accgaagaaa cgcgttccgg cgaatccgga
    10321 aaaagtgctg aagctgacag gcgcacgcgg taataacctg aaagacgtga cgctgacgct
    10381 gccagtcggt ctgtttacct gcatcacagg ggtttcaggt tccggtaaat cgacgctgat
    10441 taacgacaca ctgttcccga ttgcccaacg ccagttgaat ggtgcgacca tcgccgaacc
    10501 ggcaccgtat cgcgatattc agggactgga gcatttcgac aaagtgatcg atatcgacca
    10561 aagcccaatt ggtcgtactc cgcgttctaa cccggcgacc tataccggcg tgtttacgcc
    10621 tgtgcgcgaa ctgtttgcgg gcgtaccgga atcccgtgcg cgcggctata cgccgggacg
    10681 tttcagcttt aacgtccgtg gcggacgctg cgaggcctgt cagggcgatg gcgtgatcaa
    10741 agtggagatg cacttcctgc cggatatcta cgtgccgtgc gaccagtgca aaggtaaacg
    10801 ctataaccgt gaaacgctgg agattaagta caaaggcaaa accatccacg aagtgctgga
    10861 tatgaccatc gaagaggcgc gtgagttctt tgatgcggtg ccagctctgg cgcgtaagct
    10921 gcaaacgttg atggacgttg gtctgacgta cattcgcctg gggcagtccg caaccacact
    10981 ttctggtggt gaagcccagc gcgtgaagct ggcgcgtgag ctgtcaaaac gcggcaccgg
    11041 gcagacgctg tatattctcg acgagccgac caccggtctg cacttcgccg atattcagca
    11101 actgctcgac gtactgcata aactgcgcga tcagggcaac accattgtgg tgattgagca
    11161 caatctcgac gtgatcaaaa ccgctgactg gattgtcgac ctgggaccag aaggcggcag
    11221 tggtggcggc gagatcctcg tctccggtac gccagaaacc gtcgcggagt gcgaagcatc
    11281 acacacggca cgcttcctta agccgatgct gtaatcgtta aggccgcttt ctgagcggcc
    11341 ttttcctttc agagttgcac cagcaattta cgtttttctt ccggcagtaa attcaccgcc
    11401 tgctgataag acgcatccac cagataatag atttgcgaat ctggcagcga accgtcgaga
    11461 tacacggtgc tccagtgcgc tttattcaga tggcggcttg gacgcacatc gctgtgctgc
    11521 tgacgtagca gctccgccag ctccgggctg gttttcagcg aaacagctgg gcgattttct
    11581 acttctttca ccatcgcaaa cagtacatct tcaactttga tctgcgtcgc tttccagtcg
    11641 ttatgcacgc tctgttctgc gcctggtttt gccatgcaat attgtagcaa ctccgaaatg
    11701 gtcatttttt actccccttg tagtgtcgcg atgatgcgac gcgatccgcc gtggatgcga
    11761 tgttccccca gccaaatgcc ttgccaggtg ccggtctgaa tacgcccttt atgcaccggc
    11821 aatacaagcg atgttcccag cattgaggat ttgatatgag aaggcatatc gtctgctccc
    11881 tcatagtcat gctcatagtt tccattatcg ggaacggtgc ggaggaaaaa acgctccatg
    11941 tcgtggcgta cggtgggatc gcagttctca ttaagtgtca gagaggcgga ggtatgttgc
    12001 agcaacagat gcagtaagcc gatgttaacg cgcggcatat cagccagctg attcagaatt
    12061 tcatccgtta ccagatgaaa cccacgagat ttggcgctaa gcgtgagcgt cttttgatac
    12121 cacatgtgct gctccttgat aaaactctct taatcagttt gcagcaagac agcgaaagga
    12181 taaaggtgtg attaaaaaaa cagcattcgg gagagtgtca cgctctcccg ttctgtcagt
    12241 attctgaatt gacgatcacc tcttcaccaa acgcacccgc ttgtggcaag ggtttgtagg
    12301 tagagttgga ggcgcgcaga atgcggatac cacgagcgcc gacatcgcgt gcagcggtaa
    12361 tatcgttatc ggaatcgcca taaaaaattc ggatattttt atcctgcagc cattgcgatt
    12421 ttgtattttg ccctggttta tcgcccgcaa agatcaccgg attcatgttg gtggcaggaa
    12481 tatgaaaatt atccgccagc gtttttgaaa ccgtttctgt tttcgtcggg ctacgaccag
    12541 tcacaaagaa gatcgcgtca ccacggcgta catgcatatc aatcagctgg cgagcgacct
    12601 cttttggaat gctgaattca tcccagccgt tgttcatttt ttcccagaat acagggtttt
    12661 tcagataatc ttcgctttct ggcgagaagg tttttttgcc gcgccagaag cccgggctgg
    12721 aaaaaagtac cgtgtcatcg atatcaaacc ccaccgccat tggcggacgc cctgcgaggc
    12781 tattttcaat ttgtgcgacc gaaacccaat gaattggtgc ctgttcagca agcctggcaa
    12841 cgttagtccc agggttaagc ggtgaaggag atgaggccag ggcaacagcg gaactgttta
    12901 gagcgaacaa taagcaaacg gcactgattg cctgtgtgat cttgcgcata tttttcccta
    12961 aatagtcagt atgttgaaac tttttattgt gagatttgtt gcaaaaacca tctgaccata
    13021 acgccagctg caggggaaag gaaggattat ctgcggtttt tgtgatgaaa agcaggatga
    13081 ttatgtttaa cccagtaaat acgttcctgt ctttttttgt ataaaaaatg tttattactc
    13141 gatgtaagga tacattaatt ttacgtttat tatgatgaat cctctgcaaa tgagcgaaat
    13201 ttgatagaaa atttatctgc tgtacgattt aatgaactta atgatacagc attacaaaaa
    13261 caatcgaagt ttataaaata acat
//
LOCUS       DAZFKF010000123        13280 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFKF010000123 DAZFKF010000000
VERSION     DAZFKF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.125"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..514
                     /locus_tag="ACHKA1_004425"
     CDS             <1..514
                     /locus_tag="ACHKA1_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO5681990.1"
                     /translation="NCKISNANFSNAKFYNTNCTGANCSDILFDYAWFDNTIFIKTLF
                     KNTCFYNVRVKNVYLEGAYLNNDNIVKKANNSTEKQSIDSTDKQANDSTVQQSIDSTD
                     KQANDSTEKQANNSTEKQANNSTDKQAKNSTEQQDSNSSNQARLKKEVNIRFSIPGLT
                     SYQPTYIVEE"
     gene            780..1622
                     /locus_tag="ACHKA1_004426"
     CDS             780..1622
                     /locus_tag="ACHKA1_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO5681991.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNANSEVSILKKIKIPLQETPTNCGFEGLAYSPQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKALQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVRGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            1690..2868
                     /gene="yjiJ"
                     /locus_tag="ACHKA1_004427"
     CDS             1690..2868
                     /gene="yjiJ"
                     /locus_tag="ACHKA1_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5681992.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
     gene            3079..8318
                     /gene="iatD"
                     /locus_tag="ACHKA1_004428"
                     /pseudo
     CDS             3079..8318
                     /gene="iatD"
                     /locus_tag="ACHKA1_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
     gene            complement(8442..9026)
                     /gene="kptA"
                     /locus_tag="ACHKA1_004429"
     CDS             complement(8442..9026)
                     /gene="kptA"
                     /locus_tag="ACHKA1_004429"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO5681993.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            9276..9959
                     /gene="yjiH"
                     /locus_tag="ACHKA1_004430"
     CDS             9276..9959
                     /gene="yjiH"
                     /locus_tag="ACHKA1_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO5681994.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            9956..10417
                     /gene="yjiG"
                     /locus_tag="ACHKA1_004431"
     CDS             9956..10417
                     /gene="yjiG"
                     /locus_tag="ACHKA1_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO5681995.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLTTAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            10430..11602
                     /gene="iadA"
                     /locus_tag="ACHKA1_004432"
     CDS             10430..11602
                     /gene="iadA"
                     /locus_tag="ACHKA1_004432"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO5681996.1"
                     /translation="MIDYNAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGSKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11667..12578
                     /gene="hypT"
                     /locus_tag="ACHKA1_004433"
                     /gene_synonym="qseD"
     CDS             11667..12578
                     /gene="hypT"
                     /locus_tag="ACHKA1_004433"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO5681997.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(12571..12963)
                     /gene="iraD"
                     /locus_tag="ACHKA1_004434"
     CDS             complement(12571..12963)
                     /gene="iraD"
                     /locus_tag="ACHKA1_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO5681998.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLVISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12960..13043)
                     /gene="idlP"
                     /locus_tag="ACHKA1_004435"
     CDS             complement(12960..13043)
                     /gene="idlP"
                     /locus_tag="ACHKA1_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO5681999.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(13159..>13280)
                     /locus_tag="ACHKA1_004436"
     CDS             complement(13159..>13280)
                     /locus_tag="ACHKA1_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682000.1"
                     /translation="SFPPELSSGGDKYFHLRNYSEYSEYTSGFFLSLMIFIKS"
BASE COUNT         3496 a         3310 c         3204 g         3270 t
ORIGIN      
        1 aaattgtaaa atatctaatg caaacttttc gaatgcaaaa ttctacaata ctaattgtac
       61 tggtgcaaat tgttcggata tcctatttga ctacgcatgg tttgacaata caatatttat
      121 aaaaacgctt tttaaaaata cctgttttta caatgtcaga gtgaaaaatg tctatcttga
      181 gggagcatat ctgaacaatg ataatatcgt gaaaaaagcc aataacagta ccgagaaaca
      241 atccattgac agtaccgata aacaagccaa tgacagtacg gtgcaacaat ccattgacag
      301 taccgataaa caggccaatg acagtaccga gaaacaagcc aataacagta ccgagaaaca
      361 agccaataac agtaccgata aacaagccaa gaacagtacc gagcaacagg acagtaacag
      421 ttctaatcaa gcccgtttaa agaaagaagt aaatataaga ttttccattc cgggtttaac
      481 gtcttatcag ccaacgtata tagttgaaga atagttcaat aatcacccaa acagagcagg
      541 tatggcagag gatataaatt aacatttaaa cgttaaccgc ctcatctatc attcatggat
      601 gaggcgcatt tatcagtcga tgtaagctga aaatgttctg ccatccagag acaaccgccg
      661 ccccctgcgg cgtttactat ttcatcgccc gcgttaagaa ttcattaagc ttcatccgta
      721 tactccttat ctgtcgcact gaatttgacg agaaaaaagc aataataagg agcaataagt
      781 tgaaaaagcg catcaccttt atcgtttttc tttgcgccat cgtggcggca agcctgtttt
      841 ttgtgcaatc ctgcgtccgt aaaagccagc atgtcgcagg gtttcagaat tatcaggcca
      901 ctatcgatgg taaagagatt acaggagtaa ctaaaaatat ctcctcactg acctggtcag
      961 cgcagagcaa taccttattt agcactatca acaaacctgc aaccatcgtt gaaatgacga
     1021 ccgagggcga cctgatccgc accattccgc tcgattttgt taaggatctc gagacgattg
     1081 aatacattgg tgataataaa tttgtcatca gtgatgaacg tgattacgcc atttatgtga
     1141 tttcacttaa cgctaactcg gaagtgagca tcctcaaaaa aattaaaatc ccattgcagg
     1201 aaacgcctac taactgtggc tttgaaggac tggcttactc ccctcaggac cataccttct
     1261 ggttttttaa agagaaaaat ccgatagagg tctacaaagt aaccgggcta ttgcgtagcg
     1321 atgagttgca tatcagcaaa gataaagcgt tgcagcgtca gtttacttta gacgatgttt
     1381 ctggcgcgga atttaaccca cagaaaaata cgttgctggt gttgtcacat gagtcacgcg
     1441 ccctgcagga agtgaccgtg agaggtgacg tgatcggcga aatgtcgtta accaaaggta
     1501 agtatggcct ttcacacaac atcaagcagg cggaagggat agcgatggat gatagcggca
     1561 acatctatat cgttggcgaa ccgaatcttt tttatcgctt tacgtcgaca aaatcacgat
     1621 aacagtctgg caggttctcc ttttttgtta ttaacttgtt aactcaaaat aatacaaacc
     1681 aggcagaaca tgaattcgac atcgcatccc gtagaacgtt tttctttcag caccgcgtta
     1741 ttcgggatgc tggttctgac cttaggtatg ggtttaggcc gctttctcta tacgccgatg
     1801 ctgccagtca tgctggcgga aggcgagttt tcctttagcg aactctcatg gatcgccagt
     1861 ggtaactatg ccgggtatct ggcggggagc ctgctgtttt cattcggcgc gtttcattta
     1921 ccctcacgcc tgcgcccgtt cctgttagct tccgccctcg caaccggatt attaatcctc
     1981 gcgatggcgt ggctgccgcc gtttcttctg gtttttatca ttcgctttct ggcgggagtc
     2041 gccagcgccg gaatgttgat tttcggctca acgctcatca tgcaacatac ccgccatccc
     2101 tttgtccttg cggcgctatt ttctggtgtt ggcgtcggga ttgctctggg caatgaatat
     2161 gtgctggcag gcctgcattt tgccctctct tcacaaacgt tgtggcaagg tgccggagca
     2221 ctttctgcca ttatattgct tgctctggcg ctgctcatcc cgtcgaataa acacgttatc
     2281 ccgccagcgc cattggcaaa aatcgcgcaa caacccatga gctggtggtt actggcgatt
     2341 ctgtatggtc tggcgggttt tggttatatc atcgtcgcca cctacctgcc gctcatggcg
     2401 aaagacgcgg gccagcctgt gttgacggct cacctctgga cactggttgg cttgtcgatt
     2461 gtcccaggtt gctttggctg gctgtgggca gccaaacggt ggggagcatt accttgcctg
     2521 accgcgaatt tgctggtgca ggcgatctgc gtgctgttaa ccctcgccag cagctctcct
     2581 ttattactca tcatcagcag tattggtttt ggcggcacct ttatgggaac gacctcgctg
     2641 gtgatgacca tcgcccgcca gcttagcgtg ccgggaaatc ttaacctttt gggctttgtg
     2701 acactcattt atggtatcgg gcaaattctt ggcccggcgc tgaccagtat gctcggcaac
     2761 ggaacgtcgg cgctcgccag tgccacactc tgcggcgcag cggcgctatt tatcgcagca
     2821 ttaatctgcg ggatgcaaat attcaaattg catacgaatt attcttaagt aatttaacga
     2881 atttaacttt tcagtacatc acgtaaaata attaaaacat cctttttctt tggaggtact
     2941 cagccaccgg cgttattagt cctggtttag caattttacg gctaattcat tatttctggt
     3001 cacattatag tcaggatcgc tttatatctc cgtgctagaa aaaacaagtt taattctgtt
     3061 ttttcacgga aggtcactat ggcttcgaca aatgcgcatc aaataaaaaa taatgatcaa
     3121 aactctttat gtggtctggg tgacaaaata cgccgcctga ccgccggagt ttgtttattt
     3181 acacaaattt ttttccccgt catggcgacg gcacaaaatg tggtacatgc taaacctcag
     3241 acgacagttt catctgcccc cctctaatag aaaataatac ggtgccttac acgcttggtg
     3301 cgctggaatc agcgcaaagc gttgctgatc gattcggtat ttcactggag gagcttcgtc
     3361 gtcttaatca gttccgtact tttgctcgcg gctttgataa cgtgcgccag ggtgaggaac
     3421 tggatgttcc ggcaacaacc tcgcagaaaa gccatgagca acaaaatgcc ataccacctg
     3481 cgaatggcga aaacacgctg gagaatcaaa tagccagcac ctcgcagcga gttggcactc
     3541 tgctttcaca agatatgaat agtgagcagg ccagcggcat ggcgcgtggt tgggcgtctt
     3601 cagaagcctc aggcgcgatg actgattggt taaataactt tggtactgcg agaatctctc
     3661 tgggtgtgga tgaagatttt agcctgaaaa attcgcaatt cgacttcctg catccgtggt
     3721 atgacacacc tgattatctg ctcttcagcc agcataccct tcaccgaaca gacgatcgta
     3781 cccagatcaa caccggtttg ggctggcgtc atttcacctc cagctggatg tcaggcatca
     3841 acctgttttt tgaccacgac ctgagccgct atcactcccg cgcagggctt ggcgcagaat
     3901 actggcgtga ttatctgaag ttgagcagca acgcttatat cggcctgacc ggctggcgta
     3961 gcgcaccaga attggataac gacttcgaag cccgcccggc caacggctgg gatttacgcg
     4021 cggaaggctg gttacctgcc tggccacaac tggggggaaa actggtctat gaacaatact
     4081 atggcgatga agtggcgctg tttgacaaga atgatcgtca aagtaacccc catgctatta
     4141 ccgcaggcct caactatacc cccttcccgc ttctgactct cagtgcggaa cagcgtcagg
     4201 ggaagcaagg tgaaaatgac acacgttttg ccgttgatct gacctggcaa cccagcagtt
     4261 caatgcagaa acagcttaac ccggacgaag tggccggacg gcgcagtctg gccggtagcc
     4321 gttatgacct gattgatcgc aacaacaaca tcgttctgga atatcgcaag aaagagctga
     4381 ttcgcctgag tctgctggat ccagtgaaag ggaagtctgg agaaataaaa ccgctggttt
     4441 cctcgctaca gaccaaatat gccctgaaag gctataacat cgaagccgct gcgctggaag
     4501 ctgccggagg taaagtcagc acgtctggaa aagatatcac ggtcacgctg ccaggttacc
     4561 gcttcactaa caccccagaa accgataata catggtcgat agacgttacc gccgaggatg
     4621 taaaaggtaa cctgtctcgg catgaacaaa gcatggtagt tattcaggct ccgacattaa
     4681 gccagaaaga ttctctatta tccgttaatc cgctaaccgt ggctgcagat aaaaaatcga
     4741 cgaccacatt gaccgttact gcgcacgatt ccgacggaac tccggtgccg gggctggcgc
     4801 tgcaaacccg cagtgaaggc gttcaggata tcaccctgtc tgactggaca gataacggtg
     4861 atggtagtta catacagatg ctgaccgccg gaacgacatc aggttcagta acactgacgc
     4921 cgcaaattaa cggtgagagt gcggtaaaag aatccatcgt cgttaatatc gtccctgttg
     4981 tctcatcccg cgaccattca tcaataacaa ttgataacgt atcgtattat gccggagacg
     5041 acatcaaggt tagggtggaa ctgaaagacg aaagtaatca accggttgca tatcaaaaag
     5101 aggaattggt aaaagccgtt actgtcgaaa acagcaaacc tggcaccacg attgtctggc
     5161 acgaagagca gccgggcgtt tataccgcga attatccggc ccataagcaa gggactgcac
     5221 taagggcaca acttagcctt cacaactgga atgctccact gcaatcgcat atttataaca
     5281 ttgaggcaaa ccagaataag gctcgcgtcg ccacattatc agcgacaaat aatgacgttt
     5341 acgccgataa aaagacattt aataccctca ctatcaacgt cactgatgag agtgataatc
     5401 ccctgacaaa tcatcaggtc acctttaaga atgaaaaagg aagcgcggaa tttgtcgaac
     5461 cgccgcagca aaatacggat gcatatggtg ttgccacaat caacatggta agtcaggttg
     5521 cggaagaaaa tacgattagc gccacgctgc caaatggttt ctcacaacgg ataattgcga
     5581 aatttgttag cgattcgagt acgccaaaat tcaaacaact ggttgccgat ccagatacca
     5641 ttattgctgg caacagccag ggcagtactc tgaccgccac cgtcacagac tttcataaca
     5701 acccgttaaa agatatgaaa gtgaattttg tggcacctgg tggctcgcaa ctggacaaca
     5761 cgtccgccac aacagaccag tccggtattg tgcgggtgca cctgaccagt tcaaaagctg
     5821 gtagctattc cgtcgatgcc tcgcttgagg tggataaaaa tattcaccag tcggtcacga
     5881 tcaccgtggt cccaaacagg gaacaatcgg taatgacctt gaatgccggg tcgggcagtg
     5941 cgatcgctaa caatacaaat accgttaccc tgactgccag tgtgaaagat gtttatggac
     6001 acccgttgcc ggatgaggat gtgaaattta ccttgccagc ctccatgacc gggaacttca
     6061 cgctaagtag tgaaaccgcc cgcaccgatg caaacggtga tgccgtggtc acattgcgag
     6121 gcacaaaagc gggtgagttt acagttacgg cgacgctgac cagaaataat accgttgctt
     6181 atcagcaagt cacttttatt ggggatacaa acagtgcgca gctccagccg ctgactgcct
     6241 cattaaattc cattgttgcg ggtaacagta cggggagtac cctgacggca acgatcctgg
     6301 acgcttacca aaatccgctt aaagaccagt aggtcacatt ccagagtaac gatgtcactc
     6361 taagcggaac agaagtcacc accaatacgc taggtcaggc gacggtaaca atgaccagca
     6421 atattgccgg gcaacataac gtcgtggtga gccggaaagc gcaagcctcc gataataaaa
     6481 cgtttagttt atcagtgctc ccggatgaaa gttcggcgaa ggtaataagt ataaccggag
     6541 ccgaaaaaac gataacggtt ggcgaaaaca tcacgctacg gatactcgtc caggacgcgt
     6601 ttaacaatgt aatagcgggt caacgcgtca gattaagtgc gcagccaaca gctaacatta
     6661 cgataggcga tacggcttac accgataata acggttatgc gtacgttaac cttattagca
     6721 cccagcctgg ggtttatcag gtgacggcaa cgctggacaa taacagtagt agtaaggttg
     6781 acgtgaatgt ggcaaatggc aaactcgagt taacatcatc gaaaccagaa accacggtcc
     6841 ataatagtga gggtattacg cttaccgcaa cggcgagaaa tgcgcggggt gaattgatgc
     6901 cagggcaaat tatcaccttt agcgtaacgc ctgaaggtgc aacgctaagc aatacagggg
     6961 aagtccttac tgaccagtca ggtcaggcca aagtgacgct gaccagtgac aaagtgaatg
     7021 tctataccgt tacggccata atgggcaaag atgttcccgt tcagagccag gtaacggttg
     7081 cggttaaggc agatgctaaa acggcacatg ttgtgagcgt cgtggcttct cctgacacca
     7141 tcaccgccga cggcatcgat agcagcacca tcacttcacg agtagaagat gattacggat
     7201 tcccggttga aggtgtcgat gttagtcatg gcttagacac caaaggcagc ccggtagtta
     7261 atattccaac tacgcgtacc gatcagtccg ggcaagtcac ggcgacaata accagtacat
     7321 tggcagaaac cttaacagtc aatgtgcaag ttcctggtac agccaaccaa tccgcaacca
     7381 ttacattggt tgccggcacg gccgatgaaa gtaagtcaat tttgaaatcc gatgttggca
     7441 ctctgaaggc tgactaccag cagagcgcaa aacttacgct aacattgcaa gacaagtacg
     7501 gtaacccgat agtgacgtct gatcatctgg aatttgtcca gtcaggcccc ttcgtgaact
     7561 ttctcaagtt gagcgatatt gattacagcc aaagaaatta tggcgagtac accgtgactg
     7621 tcactggcgg aaaagagggg acagcgaccc tcattcccat gctgaacggg gttcatcagg
     7681 caaacttaag catatcgctg aatctcatcc aatcgataaa agaaatgtcc ggtcatgtca
     7741 ctgcaaacaa ccataccttc tccacggcta aattcccgag cgaaggcttt gcaggggcgt
     7801 attacacact caacaatgat aactttgaag cgggtaaaac cgttgatgat tatatgtttt
     7861 caagttcaca gggttgggtg tctgtcgatg cttcaggtaa agtttctttc gcaaatatcg
     7921 gcgatcaaac gtcagtcaca ataagcgctg ttccccgaca aggaggtaca acctaccaga
     7981 ccttaattaa gctgaaaggc tggtgggtga ataatggaaa tcataccaat atctggctag
     8041 ctgccaatgc gctctgtcat gctaaaaatg atggatataa tcttcctggc atcacacatt
     8101 tgacgtctgg cgaaaacaaa cgcacgcagg gatcactgta tggtgaatgg gggaacgttg
     8161 gagcgttttc cagtaattcg caatttacac cgggagctta ctggacaagt gaacctgatg
     8221 attacagtcg gcactactat gtgcagatgc taaccggtat gaccggaagc gacgctgatt
     8281 ccagccccca actgaccgcc tgccgtaaat cactttaatt tcagcagttg ttattaaagg
     8341 ataccctttt acgagggtat cctttcagac tgataacaaa cgctacattg ccggatgtgc
     8401 tgcgcttatc aggcctatat agcccctgca atatattgaa tttattgttt ttgtacccca
     8461 gtcggcataa acgaccactc aagaaactca acagcaaccg ttgatgtcag ccagaccccg
     8521 ttttccgctt gccagaaagg gatgcctcgt ttcgccatct cctgtgcttt gacggttaag
     8581 ataaccggcg atccgtgtct tgctcccact ttgcgtgccg ttgcttcatc agcagagaga
     8641 tgcacgtaat gacgatcccc tgcaatcagc ccctgttttt ttatctcgtc aaggaaacgg
     8701 cttgccgtac cgtggtagag aaactgtggc ggtgtttttt cggcaaagga aatagcgacc
     8761 tgcgaggtcg aatgcccctg aaccgcgcgg atgcacctac catcactgga ataactaaaa
     8821 cgctttttat cgctggtggc gaccacggta tccagcaatg cacgggttaa tcgcttgccc
     8881 gctttttggg cacaaagtat gagcttatcg atatccgccc atccttcacg gtccagtacg
     8941 ataccaatcg cctccggttt gtgtcggagg acaaaactta aaaacttgct cgtatccgcc
     9001 agctcttttt cgttgtactt tgccatcgcg ttttcgtccc tggattttct gtttttattt
     9061 accaatgtca ttgtcactgg gtttatgaat tttctgcaac tcaatcctgc gtagaacctt
     9121 ttccacacag cgtaattttt ccgctatgca aaaaacgcat agccagaaac atacttgcat
     9181 tttccccata cccacatggc tgataaggtt tttccgctcc aggaattttc ctggctcaag
     9241 caacacacaa acacaaaaaa acaatgggga ttgttatgac acaacaaggg gatgctgtag
     9301 ccggggagct ggcgacagaa aaagtgggca ttaaagggta tctggctttt tttctcacca
     9361 taatcttttt ttccggtgtt ttttccggaa cagacagctg gtggcgcgtt tttgattttt
     9421 ccgttctgaa cggatcgttt gggcaactgc ctggtgcgaa cggagccacc acctcatttc
     9481 gcggcgcggg tggtgcgggg gctaaagatg gctttctctt tgcgctggag ctggcaccgt
     9541 cagtcattct ctctctgggg attatttcaa ttaccgatgg tcttggtgga ttacgcgccg
     9601 cgcagcagtt aatgacacct gtgctaaaac cgttgttggg cattccaggg atctgctcgc
     9661 tggcgctgat cgctaacttg caaaacactg atgccgccgc cggtatgacc aaagagctgg
     9721 cgcaggaagg tgaaattacc gaacgcgata aagtgatttt cgccgcatat caaaccagcg
     9781 gtagcgccat catcaccaac tacttctctt ccggcgtcgc ggtgtttgcc tttctgggca
     9841 cgtccgtaat cgtaccttta gccgtcatcc tggtgtttaa atttgtcgga gccaacattt
     9901 tgcgcgtctg gcttaatttc gaagaacgtc gcaacccaac gcaaggagcg caagcatgac
     9961 aactcaggtc cgcaaaaacg tcatggatat gtttatcgat ggcgcgcgcc gtggctttac
    10021 tatcgccacg accaacctgc tgccaaacgt ggtgatggcg tttgtcatca ttcaggcgct
    10081 gaaaataacc ggcctgctcg actgggtggg gcatatctgt gaacccgtaa tggcgctgtg
    10141 gggattaccc ggtgaagcgg caaccgtcct tttagccgca ttaatgagta tgggcggcgc
    10201 agtcggtgtt gccgccagtc tgacaaccgc tggcgcatta accggtcacg atgtcaccgt
    10261 tttactgccc gcgatgtatt taatgggcaa cccggtgcaa aatgtcggtc gctgcctggg
    10321 gacagccgaa gtgaatgcca aatattaccc gcatatcatt acggtctgcg tgattaacgc
    10381 attgctgtcg atctgggtta tgcagcttat tgtttaacaa ggagttacca tgattgatta
    10441 taacgcagcc ggttttaccc tgctgcaggg agcgcatttg tatgcgccgg aagatcgggg
    10501 aatttgcgat gtcctcgtcg ctaacggcaa aattatcgcc gttgccagca atatcccttc
    10561 tgacattgta ccgaactgca cggttgtcga tctcagtggg cagatcctct gcccaggttt
    10621 tattgatcaa cacgtccatt tgattggcgg tggcggcgaa gcaggtccca cgacgcgcac
    10681 gccggaagtg gcgctaagtc gcctgacgga agcgggcgtc acgtcagtgg ttggtctgct
    10741 gggcaccgac tctatctctc gccacccgga atccctgctc gccaagaccc gtgcgctcaa
    10801 tgaagaaggc atcagcgcct ggatgctgac cggcgcttat catgtccctt cccgcactat
    10861 tacgggttcc gtggaaaaag acgtggcgat tatcgatcgt gtgattggcg tgaaatgcgc
    10921 catctctgat caccgttctg ccgcaccgga cgtttatcac ctggctaata tggcggcaga
    10981 atcccgcgtt ggcggtttgc tcggcagtaa acctggcgtc accgtgttcc acatgggcga
    11041 cagtaaaaag gcgttacagc ctatctatga cctgctggaa aactgcgatg tgccgatcag
    11101 caagctgctg ccgacccacg ttaaccgcaa cgtaccgttg tttgagcagg cgctggagtt
    11161 cgcgcgcaaa ggcggcacca tcgatatcac cagcagcatt gacgaaccgg tcgcccctgc
    11221 cgaaggtatt gcccgcgccg ttcaggcggg tattccgctg gcacgcgtca ccctcagctc
    11281 cgacggcaac ggtagccagc cgttcttcga tgatgaaggg aatttaaccc atatcggcgt
    11341 tgcaggtttt gaaacgttgc tggaaaccgt gcaggtgctg gttaaagact atgatttcag
    11401 tatcagcgat gccctgcgcc cgctcaccag tagcgtagcc ggtttcctta acctgagcgg
    11461 gaaaggcgaa attctgccag gcaatgacgc agacttactg gtcatgacgc cagaattgcg
    11521 cattgagcag gtatacgctc gcggcaaact gatggtcaaa gacggcaaag cctgcgtgaa
    11581 aggaacgttt gaaacggctt aaacggttgt cagccgccct aatggacgta gaatgcccgc
    11641 aagggcggct gacagagtaa aacgtaatgg atgactgtgg tgcggttttg cataatattg
    11701 aaaccaaatg gctttatgat tttctgaccc tggaaaaatg ccgcaatttt tcccaagcgg
    11761 cagtcagtcg taacgtctcg caaccggcat tcagccgccg catccgcgcg ctggaacagg
    11821 cgattggcgt tgaattgttt aaccgccagg tgacgccgct gcaactctca gaacaaggga
    11881 aaatcttcca ttcgcagatc cgccatctgt tgcaacagtt agaaagtaac cttgcagagc
    11941 tgcgtggcgg cagcgattac gcgcaacgta aaatcaagat agccgctgca cactctcttt
    12001 ccctcggtct gttaccgtcc attatcagcc agatgccgcc gctctttacc tgggcaattg
    12061 aagccattga tgtcgatgaa gcagtcgata aactgcgtga agggcaaagt gactgtattt
    12121 tttcctttca cgacgaagat ttgctggaag cgccgtttga ccacattcgc ttatttgaat
    12181 ctcaattgtt ccccgtttgc gccagtgacg aacacggaga agcacttttt aacctcgcgc
    12241 agccacactt tccgttactg aattacagcc gcaactccta catggggcga ttgattaatc
    12301 gcaccctgac gcgccacagt gagttaagtt tcagcacctt ttttgtctct tcgatgagcg
    12361 agcttttaaa gcaggttgcc ctcgacggct gtgggattgc ctggctgccg gaatacgcca
    12421 tccaacaaga aattcgcagc gggcagctcg ttgtgctgaa tcgggacgaa ctggtgatcc
    12481 cgattcaggc ctacgcatac cggatgaata cccgcatgaa tcccgttgcc gaacgcttct
    12541 ggcgtgaact gcgcgagctg gagattgtgc ttagctgaca ttctcaagcg tcgcactgcg
    12601 ccaaccgcca ttatcccaga ccagatcaaa cgttagcggt tcatcgtcgt ggtacagcat
    12661 tatctcaagg ctgatcacca gcgtgtggca gtacgcttct ttcaaattaa cctgcaacgc
    12721 cgcaatccgt ggctcatgaa ataacagtgc agaacgcagc tgacggcaat accagctctg
    12781 gagatgtttg tcgctcgggt tatgcacttg ccactcgggc atgcctgaaa caagtaatga
    12841 cggtaatgcc gaatgaggag aattaaaaag agtgaggaga tcgctgcata ccgatttacg
    12901 cagcaatgac gttttattgc ctgaaatttc aggtaaaaca gcctgaagtg attggcgcat
    12961 cattttgcgc actcctgacg tttagcaaca tatgcggctt gccctgaaca ccgggcacca
    13021 ctgtattgat gctcattttc catagccatt cccttttgtt cattacatcc atgtcagtct
    13081 gagacaggcc tgaatcctgt cagcaaagta taaaacctta cttgcctatg aatatctact
    13141 gacttttcag gaaaaaagtc atgatttgat aaaaatcatc aaactcaaaa aaaaaccgct
    13201 agtatattct gaatattccg aatagttacg tagatgaaaa tacttgtcac cgccgctact
    13261 taattccggt gggaaactat
//
LOCUS       DAZFKF010000124        13239 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFKF010000124 DAZFKF010000000
VERSION     DAZFKF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.126"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..226)
                     /locus_tag="ACHKA1_004437"
     CDS             complement(<1..226)
                     /locus_tag="ACHKA1_004437"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682001.1"
                     /translation="MPEQVITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEIIKT"
     gene            163..1662
                     /gene="pitB"
                     /locus_tag="ACHKA1_004438"
     CDS             163..1662
                     /gene="pitB"
                     /locus_tag="ACHKA1_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO5682002.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLITMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1711..2403)
                     /gene="yghT"
                     /locus_tag="ACHKA1_004439"
     CDS             complement(1711..2403)
                     /gene="yghT"
                     /locus_tag="ACHKA1_004439"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5682003.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVVKLPLMGKSLNKSIERNKVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACMRKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2577..3290
                     /gene="yghS"
                     /locus_tag="ACHKA1_004440"
     CDS             2577..3290
                     /gene="yghS"
                     /locus_tag="ACHKA1_004440"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5682004.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPIIGASFGRYLRGKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3322..4080
                     /locus_tag="ACHKA1_004441"
     CDS             3322..4080
                     /locus_tag="ACHKA1_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682005.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4126..5475
                     /locus_tag="ACHKA1_004442"
     CDS             4126..5475
                     /locus_tag="ACHKA1_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO5682006.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAAHELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5475..6299
                     /locus_tag="ACHKA1_004443"
     CDS             5475..6299
                     /locus_tag="ACHKA1_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682007.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLIRKQAKGDELTTDERKQFKYMRARYKHLRFAQRLYL
                     KKHQARFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            6311..6871
                     /locus_tag="ACHKA1_004444"
     CDS             6311..6871
                     /locus_tag="ACHKA1_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO5682008.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLDKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6902..7972
                     /gene="lptF"
                     /locus_tag="ACHKA1_004445"
     CDS             6902..7972
                     /gene="lptF"
                     /locus_tag="ACHKA1_004445"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO5682009.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTDNRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWIVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7969..9048
                     /gene="lptG"
                     /locus_tag="ACHKA1_004446"
     CDS             7969..9048
                     /gene="lptG"
                     /locus_tag="ACHKA1_004446"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO5682010.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSLGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQAMQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKMWILHGVNHKKWLNGKETL
                     DTSDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPIAFVLVALSLVYWYDRQY"
     gene            complement(9083..10255)
                     /locus_tag="ACHKA1_004447"
     CDS             complement(9083..10255)
                     /locus_tag="ACHKA1_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO5682011.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIASQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     DGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10255..10503)
                     /locus_tag="ACHKA1_004448"
     CDS             complement(10255..10503)
                     /locus_tag="ACHKA1_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5682012.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10535..11449)
                     /locus_tag="ACHKA1_004449"
     CDS             complement(10535..11449)
                     /locus_tag="ACHKA1_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO5682013.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTHCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGIAGGYDW
                     QRVQQLAAEARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11446..13134)
                     /locus_tag="ACHKA1_004450"
     CDS             complement(11446..13134)
                     /locus_tag="ACHKA1_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO5682014.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKAR
                     AEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHTWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQESLA"
BASE COUNT         3180 a         3267 c         3443 g         3349 t
ORIGIN      
        1 gtgttttgat tatttctaaa ggttttgaat gaatgcttat tgtctgatac acaagaaata
       61 acactctttt tatcgttaaa aaatgatatt tcactttgcc catgccgtta aagtcactga
      121 taatgcgtcc gttcgtaaat tcaaaatggc gtaatctaat atatgctaaa tttatttgtt
      181 ggccttgata tatacacagg gcttttgtta ttacttgctc tggcatttgt gttgttctac
      241 gaagcaatca atggttttca tgacacggcg aatgcggtgg caaccgttat ttatactcgt
      301 gccatgcaac cacaacttgc tgtggtgatg gcggcatttt ttaacttttt tggcgtgtta
      361 ttgggcggac ttagcgttgc ctatgccatt gtccatatgt taccaaccga tttgttgctg
      421 aatatggggt caacccacgg tctggcgatg gtcttttcca tgctgctagc ggcgattatc
      481 tggaacctgg gaacgtggtt ctttggttta ccggcctcca gttcgcatac cttgattggc
      541 gcgattatcg gcatcggttt aaccaacgcg ctgttaaccg gctcatcggt gatggatgcg
      601 ttaaacctgc gtgaagtgac caaaattttc tcctcgctga ttgtttcccc tatcgtcggc
      661 ctggtcattg cgggaggcct gatattcctg ctgcgacgct actggagcgg aacgaaaaag
      721 cgtgaccgta ttcaccgcat tccggaagat cgcaaaaaga aaaaaggcaa acgtaagccg
      781 ccattctgga cgcgtattgc gctgattgtt tccgctgcgg gcgtggcgtt ttcacacggc
      841 gctaacgacg ggcagaaagg gatcggcctg gtgatgctgg tactggtggg gattgcccct
      901 gctggcttcg tcgtcaatat gaacgcgtcc ggctatgaaa ttacccgtac ccgcgatgcc
      961 gtcaccaact tcgaacacta cctgcaacag catcctgaac tgccgcagaa gttgattacg
     1021 atggaacctc cattgcctgc ggcatctact gatggcgcgc aagtgacgga gtttcactgt
     1081 catccggcaa atacctttga tgcgattgcg cgcgttaaaa cgatgctgcc aggcaatatg
     1141 gaaagttacg agccgttaag cgtgagtcag cgcagccagt tgcgccgcat tatgctgtgc
     1201 atctctgata cctccgcgaa gctggcgaaa ctgccaggcg tcagtaaaga agaccagaac
     1261 ctgctgaaaa aacttcgcag cgatatgtta agcaccattg agtacgctcc ggtgtggatc
     1321 atcatggcgg tagcactggc gctcggcatt ggcaccatga ttggctggcg tcgtgtagcg
     1381 atgaccatcg gtgagaagat tggtaagcgc ggcatgacgt atgcgcaagg catggcggca
     1441 caaatgacgg cggcagtgtc tatcggtctt gccagttata ttgggatgcc cgtctccaca
     1501 acacacgtcc tctcgtctgc agttgcaggg acgatggtgg tggacggcgg tgggttacag
     1561 cgtaaaacgg taaccagcat cctgatggcg tgggtattta ctttaccggc ggcgattttt
     1621 ctttctggtg ggctgtactg gatagcattg cagttgattt aaccttcctg aaaatgcccg
     1681 gtcctggcga tcgggcattt ccatttttgg ctagtgataa ccacgcgcgg tcataaaatc
     1741 cgtaatcgct ttttctgcat caaccagcac ctgttccagt ggctgattgg catcgatatc
     1801 aaccagttgt gcaccaccaa aggttaactg tggcgttatg gcaatcttcc tcgccagcga
     1861 ttcccgttta tggtcgggtt tacgcatgca ggcaacttca aggtcaacat tgagtttgat
     1921 gaccagatca ggcttatggc tcgccatcca gtgaaacgct ttacgttcct ggcttgccag
     1981 ccatgagaca aaacgaccac cttcaacgtt aggtgggaac accgtaccat cgtaagcgcc
     2041 aggaatttgg tcctgaggat aacggtcggt tagaacaatt aacccgcgac gacgacaggc
     2101 aagcatatga cgaaagcgca gtaagcgacg ggcgacaaac gctgtaatta ccagcgccgg
     2161 aactggtcct ggcaattttt ttgctgtttt cactttattg cgttcaatag atttattaag
     2221 cgattttccc attaagggca gttttaccac tgcacgaccg acatttccgg cttgttttcc
     2281 gagatgaact ctttctgcgg cgccatattt ttcgacaacg gtaatgagat gttcacacac
     2341 cgttgacttg cctgaaccat cgctaccaat aacggctatt aatggaggtg ttattgactg
     2401 catgaatata tccttaaatt taaatatcca tccaaaatat ttatctggtt aatatgtttt
     2461 tatgaaagcg taattcaggt caatgtcaca attaaccatt gtcacaataa ggttgaacgg
     2521 atattctgag cacggaccac gtaatattca atacattatt tgctgccggg tttttcgtga
     2581 atcaacgtaa tatgtcaata attaattcca cgccagtgcg tgttattgcc attgtaggat
     2641 gtgatggttc aggtaaatcg accctcacgg caagcctggt aaatgaactg gcagcaagaa
     2701 tgccaacaga acacatttat ctcgggcaat cgtccgggcg aattggcgaa tggatttcac
     2761 agctgcccat tattggcgca tcttttgggc gttatctgcg gggtaaggca gctcatgtgc
     2821 atgaaaaacc ctcaacaccg cctggcaata ttactgctct ggttatctat ctgctttcct
     2881 gctggcgggc gtacaagttt cgcaaaatgt tgtgtaaaag ccagcaaggc tttctgctca
     2941 tcaccgaccg ctacccgcag gttgaagtgc cgggttttcg ctttgatggc ccgcaattgg
     3001 caaaaaccac gggcggtaac ggttggataa aaatgttaag gcagcgcgag ctgaagctgt
     3061 accaatggat ggcgtcttat ttgcccgtat tgttgattcg tcttggcatt gatgaacaaa
     3121 ccgcgtttgc gcgtaaacct gaccaccaac tggcagcgtt acaggaaaaa atcgccgtta
     3181 cgccgcaact gacattcaat ggcgcaaaga tccttgaact ggatggtcgg caaccagccg
     3241 atgaaattct gcaagcgtca ctacgcgcaa ttcacgccgc gctttcctga tcaccaaaat
     3301 ggatagggga tgatttaacg aatggacgca ctacaaacac aaactgttaa tagtacaacc
     3361 gcgccgcagc ccaactacat tccggggctg attgcggtgg tcgggtgtga tggcaccggt
     3421 aaatccacac tgaccaccga cctggtgaaa tcgctgcaac aacactggca aaccgagcgg
     3481 cgctatctgg ggctgctctc cggcgaagac ggcgacaaaa tcaaacgatt gccgttggtt
     3541 ggcgtctggc tggaacggcg actggcggcc aaatcctcga aaacccaaag catgaaaacc
     3601 aaatctccgg cgctatgggc ggcggtgatt atgtactgct tctcgctgcg aagaatggcg
     3661 aatctacgca aggttcagcg actggcgcaa agtggcgttc tggtggtcag cgatcgcttc
     3721 ccgcaggctg aaatttcggg cttttattat gacggaccgg ggattggcgt cgaacgtgcg
     3781 accgggaaaa tcagcatgtt tctggcgcag cgcgaacggc gtttatacca acaaatggcg
     3841 caatatcgcc cggaattaat tattcgcctg ggcattgata ttgagactgc catctcccgc
     3901 aagcctgacc atgactatgc cgagctgcag gacaaaatcg gcgtcatgtc gacgattggc
     3961 tataacggca caaaaattct tgagattgat tccagagcgc cctacagcga agtgctggaa
     4021 caggcacaga aggcggtttc tctggttgcc atcgtttctg accgccgaag tttaacttaa
     4081 ggctggcggg atacttttta ctatcaacga taaggtcagg ctgaattggc gggttttaac
     4141 atcaaacatt ggtttgcaga tggcgcgttt cgcaccatta ttcgcaatag cgcctggtta
     4201 ggctccagta atgtcgtgag cgccttgctg ggtctgttgg cgctctcgtg tgccggtaaa
     4261 gggatgacgc ccgccatgtt tggcgtactg gtgattgtac aatcgtacgc caagtcgatc
     4321 agcgatttta ttaagtttca gacatggcag ctggtggttc agtacggaac accagcatta
     4381 accaacaata atccgcagca atttcgcaat gtcgtctcat tttccttctc gctggatatc
     4441 gtcagcggcg cggtggcgat tgtcggcggc attgccttac tgccattcct ttcccattca
     4501 ttaggtctgg atgaccaaag cttttggctg gcggcgctgt attgcacgct cattccttca
     4561 atggcttcct ccacgccgac cggcattctg cgtgcggtag atcgctttga tttgattgct
     4621 gtacagcagg cgacgaaacc ttttctgcgc gcagcgggga gcgtcgcagc ctggtatttt
     4681 gactttggtt ttgcgggttt tgttattgcc tggtacgtgt cgaatctggt tggcggcacc
     4741 atgtactggt ggtttgccgc gcacgaatta cgccgcagaa atatccataa cgccttcaaa
     4801 ttgaatctgt ttgagtctgc ccgacacatt aaaggcgcgt ggagttttgt ctggtcaacc
     4861 aacattgccc actccatctg gtcggcgcgt aactcgtgca gcacagtgtt ggtggggatc
     4921 gtgttaggac ccgctgccgc cgggttattt aaaatcgcca tgacattctt cgacgccgcc
     4981 ggaacgccag cgggtttact gggtaaaagt ttttacccag aggtgatgcg tttagatccg
     5041 cgcaccacca gaccgtggtt gttaggcgtg aagtccggtt tactggcggg cggaatcggt
     5101 attctggtgg cgctcgcggt gttgatcgtc ggcaagccgc taatttcgct ggtgtttggc
     5161 gttaagtatc tcgaagcgta tgacctgatt caggtgatgc tgggcgcaat tgtgatctcg
     5221 atgctgggct tccctcagga gtcattgcta ttgatggcgg gaaaacagcg cgcttttctc
     5281 gtggcgcaaa ccatcgcctc aatcggctac atcgtattgc tgttcatgtt ctgtcatctg
     5341 tttggcgtgc tgggagcagc gtttgcttat ttcggcggtc aatgtctgga tgtggtgctt
     5401 tcgctaattc cgaccttaaa ggcatttttt cagcgccatt ccttgcttta taacgcagcc
     5461 ggagagaaat cctgatgatg agaaaatatt tcccgcttga ggcgagcgag cgtctatttg
     5521 ttgcgattga agaagatgat gtcgttgatg cacaggtcag tttgccgccg actatcgcac
     5581 taagttgtac caccgaaatc atccatgata attacgcgtt atgcctgcaa ttctggctca
     5641 atggcgttga tcgtcaggaa ttacttcgtt taattcgcaa acaagccaaa ggcgatgagc
     5701 tgacgacaga cgagcgtaaa cagtttaaat acatgcgagc gcgctacaag catctacgtt
     5761 ttgcacaacg gctgtattta aaaaagcatc aggccagatt tttattcggc aaaacgaccg
     5821 tttttctggg acgttttcag gacggatttc gtaacggtaa gaaaaatatt gtgtcgtatt
     5881 acggcaacct gttacgaatt tatttaagct ctccggtctg gtcgttggtg aactattcat
     5941 tacgccatag ccagttagaa agcgtaagcg gttttattgc ctatcggcaa aagcaaatgc
     6001 ataccctgaa agagattatt gccaaaccgc gactaaccgg cagggaattt catgatgtgc
     6061 gaaaaatcat cagccagcag gtttcttatt acgacacctt aagatcgctt gatcctgaaa
     6121 ataaagaggc gttacagatt tcccgtttcc tcgccgccat taatggcctg atgggggata
     6181 aacacgacga catggtggcg gatgatatgg aaaaccgcca gtcgtatgat gcaccggtgg
     6241 cgctggatag cgatattcgc cagcgcttag agctgctgat ttcgcgtttt ccgctataac
     6301 cggagcaatg atgtcatctc gtttgattat tgcgctgatt atcatgctct tagccccggg
     6361 tgtgcaggca cacaatttcg tcactggcaa aacggttacc cctgtttata ttcaggaagg
     6421 cggcgagctg cttctgaata gcgacgacga aattcactac caaaagtgga acagcacaca
     6481 attggcggga aaagtccgca ttatccagta tatcgccggg cgtaagtcag cgaagaagaa
     6541 gaactcactg ttaattaaag cggttgaagc ggcaaacttt ccacaggatc gttttcagcc
     6601 caccaccatt gtgaacactg atgatgccat ctttggtact ggctattttg ttgttggcaa
     6661 aatcgaaaaa aataaacgcc gctatccgtg ggcgcaattc gttattgacg gcaatggaca
     6721 gggacgcgtt gcctggcgct tgccggagca gagttcaacc attctggtgc tggataaagc
     6781 cgggcaaatt cagtgggcaa aggacggcag cctgacgccg gaagaggtag atcatgtcat
     6841 tgcgctggcg cagaaactga tcaatgaata aaaaattcaa aggattgagg gcgttagtga
     6901 gatgaagctg gttgaacact acatcatgcg tggtacgcgc cgtctggtgc tgattatcgt
     6961 cggttttctg atctttattt tcgccagcta ctccgcacag cgttatctga ccgaagcggc
     7021 aaatgggaca ttagcacttg atgttgtgct ggatatcgtt ttctacaaag tgctgattgc
     7081 actggagatg ttgttacctg ttggactgta tgtgtcagtt ggcgtgacgc tagggcagat
     7141 gtataccgat tcggaaatta ccgctatctc tgcggcgggg ggcagtccgg gacgcttgta
     7201 caaagccgtt ctttatctgg cgataccgct aagtattttt gtcacccttc tgtcgatgta
     7261 tggtcgtccg tgggcttatg cgcagattta tcaactggag caacagtcgc agtcggagtt
     7321 ggatgttcgc cagttgcggg caaagaaatt taacactaac gataacggac gaatgatcct
     7381 ttcgcagacg gttgatcagg ataataatcg cctgactgac gcgctgattt atacttctac
     7441 tgacaatcga acccgcattt tccgcgcccg ttcggttgat gtggttgacc catctcccga
     7501 gaaaccgacc gttatgttgc acaacggcac tgcctatctt ctcgatcatc aggggcgtga
     7561 cgacaacgaa cagatctacc gtaatctgca attacatctg aatccgctgg atcaaagccc
     7621 taacgtcaaa cgcaaagcaa aatcggtcac ggagctggcg cgctccgcct ttcctgccga
     7681 tcatgccgaa ctgcaatggc gacaaagccg tggcctgacc gcattgttga tggcgctgct
     7741 ggccatttca ttaagtcggg taaaaccgcg gcaagggcga ttttcaacgt tattgccact
     7801 gacgttgctg tttgttgcca ttttttatgg cggcgacgtc tgccgtacgc tggtggctaa
     7861 cggtgcgatt cccctcattc ctggtttgtg gatagtaccc ggactcatgc taatgggcct
     7921 gctgatgctg gtcgcacgcg acttctcttt gctgcagaaa ttttcccgat gaatgttttc
     7981 agtcgctatt taatccgtca tctctttctc ggttttgccg ccgccgcagg gttattgctg
     8041 ccgcttttta ctaccttcaa cctgattaac gaactggatg atgtcagcct gggcggttat
     8101 cgctggactc aggcggtgct ggtggtgctg atgaccttac cgcgcacact ggtggagctt
     8161 tcgccgttta tcgccttatt gggagggatt gtcggcctgg ggcagctatc gaaaaacagt
     8221 gagcttaccg ccattcgcag cacggggttt tctatcttcc gtattgcact ggtggcgctg
     8281 gttgccggga tattgtggac tgtttcgtta ggcgcgatag atgagtgggt agcgtcgcca
     8341 ttgcagcaac aggcgatgca aatcaaatcg accgccactg cgttggggga ggacgatgat
     8401 attaccggca atatgctgtg ggccaggcgc ggtaatgaat ttgtgacggt gaaatcgctg
     8461 aacgagcagg gccagccggt gggcgtggag atatttcatt atcgcgacga tctctcgctc
     8521 gaatcctaca tttatgcacg cagtgccacc attaaagacg acaaaatgtg gatcctgcat
     8581 ggtgtgaatc ataaaaaatg gcttaacggt aaagaaacgc tggacacatc agataatctt
     8641 gcctggcaat cggccttcac cagtatggat cttgaagagc tatcgatgcc ggggaatact
     8701 ttttctgtcc gtcagcttaa tcattacatc cattatttgc aggaaaccgg gcaacccagc
     8761 agcgaatacc gccttgcact gtgggaaaaa ctggggcaac ccatcctgac cctggcgatg
     8821 attttgctgg ctgtgccgtt tacctttagc gccccgcgct cgccagggat gggtagccgt
     8881 ctcgctgtag gcgtcatcgt tggcttactc acctggatca gctatcaaat catggtcaat
     8941 ctgggattgc tatttgcgtt aagcgcacct gttaccgcgc tcggtttacc gatagcgttt
     9001 gtgctggtgg cgttgagcct ggtgtattgg tatgacagac aatattaaac ccgccgtggc
     9061 gcaacaccac ggcgggctca cattactgct tcagagtagc aaatgcctga atgatggcat
     9121 cgatttgctc acgactgtgg gcagcgttta cgctacagcg cagcagcgtg atgcctgcag
     9181 gcgcggcagg tggcaggata agattcacat aaacgcccag cgaaataagc tcgcgccaga
     9241 tgcgtaaacc gtcctctttc gaaccgatta ttaccggcac taccgggcta atatgtgaac
     9301 ttaactcata acccagtttt tgcagaccgt catacagatg gttggcatta tccatcagct
     9361 tttgccgcag ctctggttgt gaggcaatgg ttcttaacga cgagcgcacg gtcgcaatgc
     9421 aggacggaga aggagaggcg gtaaaaatat acgggcggct gccgtagcgc agcacttcca
     9481 tcgcttccga gcctacagca aaaccgccaa tcgaggccag acttttactg aaggtgccaa
     9541 cgataatatc cacatcatct tcaacgccga cagcttcagc cagtccgcgc cccgttgcgc
     9601 ctaacacgcc aaacgaatgc gcttcatcga caatcaaata accaccgaga cgacgtttga
     9661 tatcaacaat ttccgccagc ggcgcaacgt caccgagcat actgtagatg ccctcaacga
     9721 tgataatcgc ctctttcgcc cgttcaccga ggcgaaccat gcgccgttcc agatctttag
     9781 catcgttatg gcggaaacga ataatttccg cgccaccgag agaacaggcg tcataaatac
     9841 tggcgtggct gtcggcatcc agtaatacca cggcgttatg gtcggcaagt gcgctgatga
     9901 cgcctaaatt agcggtataa ccggttgaaa aaacgatagc ggtagggcga ttgaaaaact
     9961 cggcaatctc tttctctaac gccaggtgcg gcgcataact gccgtttgcc atacgagaac
    10021 cggtggtgcc ggtgccttgg gccgctaacg ctgcctgacc ttcggcgatg gcatcatgat
    10081 taaaggttaa accgaggtaa ttattggtcc cggccagaat cactttttta ttgccgatcc
    10141 ggccttcggt ggcggaatac acttcatcaa tacaggtgcc aaacggtgtt aacccgttgt
    10201 cactaaattg cagtcgctcg ccagcaaggc gcgcatattt atcgtatagc cccattattt
    10261 attctccagc catggaagta gagtttccat taattgcgca ggggttttga catccagcag
    10321 aatattaata ggaatggaaa tatcgaatct gtcttccagc atcattaaga gatccatcac
    10381 tttgatcgac tccaggccaa gatcgttgac gagatcgctg tcgggcttaa tttccacacc
    10441 gttttcgacc aaatcctgta aacaggaaag gatgtaatcc attactattt cacgatttac
    10501 catagaaacg ttaacacacc tttaaacaag acaatcaaaa tagcccgttg cgtaatgcgt
    10561 gttccaggct aatcccggga aaccaattaa tatcttcaga aatacggtta ttacttgccg
    10621 accagtcggc gtgggttaat tcacgaattt tcgagcgggt cagcataggt tctttacctg
    10681 ccaggcgact caacgcggta ctgatatccg caaggcaggt gagtaccggc agaggaatgc
    10741 caaccatccg cacagaacca caacgggctt cggcggcaag ttgctgtacg cgttgccagt
    10801 catagccgcc agcgatgcca tcgcataatt cataggtttg cgtctgtaca gtttcggcgc
    10861 ttaaccactg acccactgct tgcgcaaaat cggtgacatg caggaaagag agctgtgtat
    10921 ctggtgcacc aagtcgtggc agcaggccgc gcagcatcca gtcaaacagc ggttttaact
    10981 ctttatcgcc gggaccatac acggctgtcg ggcgaaaaac gcccagcgta atttcgtcag
    11041 ccattgccgt cagccgttgt tcggcgacgt gtttggaatt tgcgtaccag gagagctcgg
    11101 gatggcgcgc cgccagcgaa gagataaaca gaaaacgttg gcaaaagccg ctctcttttg
    11161 ctgcttgcat caggcgcagg ctgccgtcaa cgttacagtg ggtaaaaatc tcttctttgt
    11221 gtccgcgcac ttgcccggcg caatggacca ccgcgctggc tccggcaacc agttcgctaa
    11281 gcgaatgtgt atcttccagc gaaccgcgca cccaggtgag attgtcattg acgtgagcgc
    11341 gggcagtacg cgtcaatgcg cgaacgtgaa agccgcgggc gagcaggtta tcgataatat
    11401 atttaccgat aaacccggta gcgcccgtca ccgcgacagt ttgattcatg ccagggattc
    11461 ctgcacatga agactggcag cataagcctt ctgataacgt tttttcgctt ccgcacgggc
    11521 aggcttgccg gaggacgttc ggggaatact gtggggcggc aacagatcga tagccgcggt
    11581 cacgccaaat tcgctttgga tccgtgctgc cagcgcgtgg ataagctgcc cgcgacgttc
    11641 ttcgtcgctg atccgacact ggatctgcaa aatgattttt tcctgggcgg taacaaaagc
    11701 aatcgcatcg ccagaatgaa tttccggttc ctgttccgct atatattcaa tatcctgcgg
    11761 ccagatatta cggccacgaa taataatcag atctttaatg cgtccggtga catacagata
    11821 accgtccagc agataaccga ggtcgccggt gtctaaccag cccgtcgcgg caatctcgtc
    11881 ttgcgaagcc tggtcgccaa agtaaccgct catcagactg ggaccggaga tgcaaatatg
    11941 gcctaccaca cgttccgcga ccggcatacc tgcttcattg cggatttcaa tgccatgttc
    12001 tggcaacgct ttgccgcagt tgacgaaagt cgatacggcg cgtgtctctg cacccggcgc
    12061 gacggcttta ccctgatatt cgaggatgtc gcgatccact tcgttaacca caaccccgga
    12121 ggcttcatca gagaagctga cagccagcgc attttctgcc agtccgtagc acggcatgaa
    12181 agttttattg tcaaagttaa cctgacggaa acattcagcg aattgatgga gttgttctgc
    12241 ggagatgggt tctgcaccaa taccagcgac gcgccagcag gaaagatcca gttcagcgag
    12301 atctttttca ttcacgcggc gctggcacaa ttcatagcca aacggcggcg caacggaaac
    12361 ggtgccgcga tttttactga tcaatttaag ccattgcaga ggacgcatgg caaaatcctg
    12421 agtgcgcaaa taatctactg aaagttgcgt ggcgacgggg gtcaggagaa agcctaccag
    12481 tcccatatca tggtagaaag gcagccagga gacgcagcgg tcgccagggc gtaatttaat
    12541 gccgtcgtgg cttatagcac gtagattagc catcacttcg cgatgggtga taatgacgcc
    12601 acggggaaaa cgggtgctgc cggaggtgta ctggaggtag gcgatatcgt tcggaactgg
    12661 acgctggagc gcaacatcgg cttccggtaa tgccttaaac caggtgtggc ttaatacatg
    12721 caattcgggg ttgttatgcg tggcggcatt gaccagcggc aaccactcat caccagtgat
    12781 aatggcagcg ggctggcaac ttgccagtaa accctgcagt ttggcgctcc aggaatcccg
    12841 ctgaccgacg cccattggaa tcgccaacgg gacggcgact aagccggcat actggcaggc
    12901 aaaaaaagcc tctacgaacc cgctactggt ttcggcaatc agcgccacgc gatcgccttt
    12961 tttcaggttc agcgataaca accgcttcgc accagcttcg gcacgtgctt ttaacgtctg
    13021 atattccagt tgatcttcaa gttggcaacg tctgtcataa aaattcattc cagcgctact
    13081 cagagcggcg tagtccaaag catcaaccag cgttggaaaa tcggcatagc gcataggtag
    13141 ggaatgcgta aagattttat tagacatata caccacgaaa aaattggata tcaaaaataa
    13201 aatgacaata atgcggtgga ttatataggt ataaaagat
//
LOCUS       DAZFKF010000125        13154 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFKF010000125 DAZFKF010000000
VERSION     DAZFKF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.127"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            294..2531
                     /gene="nrfB"
                     /locus_tag="ACHKA1_004451"
     CDS             294..2531
                     /gene="nrfB"
                     /locus_tag="ACHKA1_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO5682015.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2518..5490
                     /gene="nfrA"
                     /locus_tag="ACHKA1_004452"
     CDS             2518..5490
                     /gene="nfrA"
                     /locus_tag="ACHKA1_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO5682016.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARISQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5491..6381
                     /locus_tag="ACHKA1_004453"
     CDS             5491..6381
                     /locus_tag="ACHKA1_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO5682017.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQRSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6564..7325
                     /gene="envY"
                     /locus_tag="ACHKA1_004454"
     CDS             6564..7325
                     /gene="envY"
                     /locus_tag="ACHKA1_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO5682018.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7371..7447)
                     /locus_tag="ACHKA1_004455"
     tRNA            complement(7371..7447)
                     /locus_tag="ACHKA1_004455"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7411..7413),aa:Arg,seq:tct)
     gene            7690..8322
                     /gene="fimZ"
                     /locus_tag="ACHKA1_004456"
     CDS             7690..8322
                     /gene="fimZ"
                     /locus_tag="ACHKA1_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO5682019.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTITVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(8325..8840)
                     /gene="sfmF"
                     /locus_tag="ACHKA1_004457"
     CDS             complement(8325..8840)
                     /gene="sfmF"
                     /locus_tag="ACHKA1_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO5682020.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADKDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVA
                     KADAIFMINYN"
     gene            complement(8851..9858)
                     /gene="fimH"
                     /locus_tag="ACHKA1_004458"
     CDS             complement(8851..9858)
                     /gene="fimH"
                     /locus_tag="ACHKA1_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO5682021.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(9871..12480)
                     /locus_tag="ACHKA1_004459"
     CDS             complement(9871..12480)
                     /locus_tag="ACHKA1_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO5682022.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGKNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(12511..>13154)
                     /gene="fimC"
                     /locus_tag="ACHKA1_004460"
     CDS             complement(12511..>13154)
                     /gene="fimC"
                     /locus_tag="ACHKA1_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO5682023.1"
                     /translation="SASAHAAGGIALGATRIIYPADAKQTAVWIRNSHTNERFLVNSW
                     IENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAADRESLFWMNVKTIPSVDK
                     NALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSRSGNYINVHNPSPFYVTL
                     VNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDYGSVTPVREVNLN"
BASE COUNT         3402 a         3344 c         3167 g         3241 t
ORIGIN      
        1 agtccttgcg gggagcgggc tttcgtaatc ttgccctgcc tgctcacctc caggcacttc
       61 cactacatca tgacctgatc ggtgccacca ctcctcttca ctatgacgtt aaccccccct
      121 ccttgacacc acgcttttgt ctacattcta cgcaaacggc aacttttata accagtgtaa
      181 aaataacgtg ccgcaatagt catacctatt aatggtaaaa agctgtcaca attcataaaa
      241 aaccttaata tacgccaccc taaacataac cagcgttaat gtaaggtttt tgtgtggact
      301 ggcttcttga tgtttttgct acctggctct acggcttaaa agtaatcgcg ataacgttag
      361 cggtcatcat gttcatcagc gggctggacg atttttttat tgatgtcgtc tactgggtac
      421 gccgcattaa acgcaagttg agtgtttatc gccgctaccc gcgaatgagt taccgcgaac
      481 tgtataaacc agatgaaaaa ccgttagcga ttatggttcc ggcgtggaat gaaacgggcg
      541 tcatcggcaa tatggccgag ctggcggcga ccacgctcga ctacgaaaac tatcatatct
      601 ttgttggcac ctaccccaac gaccccgata ctcagcgtga tgttgacgaa gtgtgcgctc
      661 gctttccgaa cgtgcataag gtagtctgcg cgcgtcctgg ccccaccagc aaagccgact
      721 gtctgaacaa cgtgctggac gccatcaccc agtttgagcg tagcgccaat ttcgcttttg
      781 ctggttttat tctgcatgac gccgaagatg tgatttcacc gatggaattg cgtctgttca
      841 actatctggt cgagcgtaaa gatctgattc agatcccggt gtatccgttt gaacgcgaat
      901 ggacgcactt caccagcatg acttacattg atgagttttc agagctgcat ggcaaagatg
      961 ttccggtgcg tgaagccctc gccggacaag tgcccagcgc aggcgtcggc acctgtttca
     1021 gccgccgcgc cgtgaccgct ctgttagctg acggtgacgg tattgctttc gacgtgcaga
     1081 gtctgactga agattacgac attggcttcc gcctgaaaga aaaaggtatg acggaaattt
     1141 ttgtccgttt tccggtggtg gacgaagcca aagaacgcga gcagcgtaaa tttttacagc
     1201 acgcacggac gtcaaacatg atctgcgtgc gcgaatattt ccccgatacc ttttcgactg
     1261 cggttcgaca aaaatcccgc tggatcatcg gcattgtttt ccagggcttt aaaacccata
     1321 aatggacctc cagcctgacg ctgaactact ttctctggcg cgaccgcaaa ggggcaatca
     1381 gtaactttgt cagcttcctc gcgatgctgg tgatgatcca gcttttgctg ttgctggcgt
     1441 atgaaagttt gtggcccgat gcctggcatt tcctttctat tttcagcggc agcgcatggt
     1501 taatgaccct gctgtggcta aactttggtt tgatggttaa ccgcatcgtg cagcgggtga
     1561 ttttcgttac tggctactac ggcctgacgc aggggctgct ttccgtcctg cgtcttttct
     1621 ggggcaacct gattaacttc atggccaact ggcgcgcgtt aaaacaggta cttcaacacg
     1681 gcgatccacg tcgcgtggcg tgggataaaa caacgcatga cttccccagc gtcactggcg
     1741 atacccgctc gttgcgcccg ttaggtcaaa ttctgctgga aaatcaggtc atcactgaag
     1801 aacaactcga tacagcactg cgtaatcgcg tcgaaggtct acgcctgggc ggttcaatgc
     1861 tgatgcaggg gctgattagc gccgagcaac tggcacaggc gctggcagag caaaacggcg
     1921 tggcgtggga atccatcgat gcctggcaga tcccttcctc gctgattgcc gaaatgccgg
     1981 cctccgtggc gctgcattat gcggtactgc cgctgcgtct ggaaaatgat gagttaattg
     2041 tcggcagtga agatggtatt gacccggttt cgctggcggc cctgacgcgt aaagtcggac
     2101 gcaaagtgcg ttacgtcatt gttctgcggg gacaaattgt cacggggtta cgtcactggt
     2161 atgcacgccg acgcggtcac gatccgcggg caatgttgta caatgcggtt cagcatcagt
     2221 ggctcacgga acagcaggcc ggtgaaatct ggcggcaata tgtgccgcat cagttcctgt
     2281 tcgccgaaat actgaccacg ctcggtcata ttaatcgttc agcaattaac gtgttgttat
     2341 tgcgccatga acgcagttct ctgccgctcg gcaagttttt ggtcaccgaa ggcgttatca
     2401 gccaggaaac gttggatcgc gtcctgacaa ttcaacgcga attacaagtt tcgatgcaat
     2461 cactattact caaagcaggt ttaaacacag aacaggttgc gcaactggag tccgaaaatg
     2521 aaggagaata accttaatcg cgtcatcgga tggtctggtt tactgctgac gtctttattg
     2581 agtaccagcg cactcgcaga caatatcggc accagcgcag aagagctggg gctgagcgat
     2641 tatcgccatt ttgttattta tccccgtctc gataaggcgc tgaaggcaca gaaaaataac
     2701 gacgaagcaa ccgccatccg cgaatttgaa tatatacacc agcaggtgcc ggataatatt
     2761 ccgctgactt tataccttgc ggaagcctat cgccattttg gtcatgatga ccgggcgcgg
     2821 ctgttgcttg aggatcaact gaaacgtcac ccaggagatg cccgacttga gcgcagtctg
     2881 gcggctattc cagttgaagt gaaaagcgtt acgacagttg aagaactgct tgcccagcaa
     2941 aaagcgtgcg atgctgcgcc gaccctgcgt tgtcgcagtg aagtcgggca gaatgccctg
     3001 cggctggcac agttacctgt cgccagagcg caactgaacg atgcgacgtt tgctgcatcg
     3061 ccggaaggaa aaacgctgcg aaccgatctg ctgcaacggg caatctacct gaaacaatgg
     3121 tcccaggcag atacgctata caatgaagca cgccagcaga acacattaag cgcggcagaa
     3181 cgccgtcagt ggtttgacgt gcttcttgcc gggcagctgg acgatcggat cctggcactg
     3241 caatcacagg ggatcttcac cgatcctcag tcatatatta cttacgcgac cgcgctggct
     3301 tatcgtggcg aaaaagcacg cctccagcat tatctcattg aaaataagcc gctgtttacc
     3361 acggacgcac aagagaaaag ttggctctat ctgttatcta aatacagcgc caaccccgtt
     3421 caggcattgg cgaattatac ggtgcagttt gccgataacc gccagtatgt tgttggcgcg
     3481 acgctaccgg tgctgttaaa agaaggtcag tacgacgcag cgcaaaaact gctcgccacc
     3541 ctccccgcca atgaaatgct tgaggagcgt tatgctgtca gcgtggcgac ccgtaacaag
     3601 gctgaagctc tgcgtctggc acgcttgctg tatcagcaag aaccggcaaa tcttacccgc
     3661 ctggatcaac taacctggca actgatgcag aacgagcagt cacgggaagc tgccgattta
     3721 ttgctgcaac gctatccttt ccagggcgat gcgcgtatca gccagacttt aatggcgcga
     3781 ctggcgtctc tgctggaaag tcatccttac ctggcaacgc cggcgaaggt ggcgatttta
     3841 tcgaaaccct taccgctggc ggagcaacgt cagtggcaaa gtcagttgcc gggtattgca
     3901 gataattgcc cggcaatagt tcgcttgctg ggcgatatgt cgccttccta cgatgccgcc
     3961 gcctggaacc gtctggcaaa gtgttatcgg gacacgctac ccggtgtggc gttgtatgca
     4021 tggcttcagg ccgaacaacg acaaccgaac gcctggcaac atcgtgcggt agcctatcag
     4081 gcgtatcagg ttgaggacta cgccaccgca ctggcggcct ggcagaaaat cagtcttcac
     4141 gacatgagca atgaggatct gcttgctgct gccaataccg cccaggcggc aggaaatggt
     4201 gcggctcgcg atcgctggct acaacaggca gaacaacgtg gactgggaag caatgccctc
     4261 tactggtggc tgcatgcgca acgttacatt cctggtcagc cggaactcgc actgaacgat
     4321 ctcacgcgct caatcaatat tgcgccttct gccaacgctt acgttgcgcg ggcgacaatt
     4381 tatcgccaac gtcataatgt cccggccgcg gtgagtgatt tgcgcgccgc gctggaactg
     4441 gaaccgaata atagcaacac ccaggcagcg cttggttacg ccttgtggga tagcggtgat
     4501 atcgcacagt cgcgggaaat gctcgaacag gcgcataaag ggcttccgga cgatccggca
     4561 ctgatccgac aactggccta tgtgaaccag cgtctggatg acatgcctgc gacgcagcac
     4621 tacgcccggc tggtgattga tgacattgat aatcaggcgc tgataacccc attgacgcca
     4681 gagcaaaatc aacaacgctt caatttccgc cgtttgcatg aggaggtcgg tcgccgctgg
     4741 acgttcagtt tcgattcttc tatcggcttg cgttccggcg caatgagtac cgctaacaat
     4801 aatgtcggcg gcgcagcgcc agggaaaagc tatcgtagct acgggcaact ggaagccgaa
     4861 taccgcatcg gacgcaatat gctgctggaa ggcgacctgc tctcagttta tagccgcgtc
     4921 tttgccgata ccggagaaaa cggggtgatg atgccggtga aaaatccgat gtccggcacc
     4981 ggtctacgct ggaagccgct gcgcgatcag atctttttcc tcgccgtcga acagcagttg
     5041 ccgctgaacg ggcaaaatgg cgcatccgat accatgctgc gcgccagcgc ctcattcttt
     5101 aatggcggca aatacagcga cgaatggcac ccgaacggtt ccggctggtt tgcccaaaac
     5161 ctgtacctcg atgcggcgca atatatccgc caggatattc aggcgtggac ggcagattat
     5221 cgcgtcagct ggcatcagaa ggtagctaac ggacagacta ttgagcctta cgctcacgtt
     5281 caggacaacg gctatcgtga taaaggcact cagggcgcgc agcttggcgg tgtcggggtc
     5341 cgctggaata tttggaccgg cgagacgcac tacgacgcct ggccgcacaa agtcagtctc
     5401 ggcgtcgagt atcaacatac ctttaaggcg attaatcaac gtaacggaga gcgcaacaac
     5461 gcgtttctca ccattggagt gcactggtaa atgcgtaagt tcattttcgt attgctgaca
     5521 ctgcttttgg tcagcccttt ttcctttgcg atgaaaggta ttatctggca accacaaaac
     5581 cgagatagtc aggttaccga tacccagtgg caggggctga tgagtcagtt acgtttgcaa
     5641 ggtttcgata ccctggtttt gcaatggacc cgttacggcg atgcatttac ccagccagaa
     5701 cagcgcacgt tattgtttaa gcgtgccgca gctgcgcaac aggctggcct gaagctgatt
     5761 gtcgggctga acgccgatcc ggaatttttt atgcaccaga aacagtcgtc cgcagcgctg
     5821 gaaagctatc ttaatcgcct gctggctgcc gatctccagc aagccagatt atggagcgcc
     5881 gcgcctggca taacgccgga tggctggtac atcagcgcgg aaattgacga cctgaactgg
     5941 cgcagcgaag ccgcccgtca gcctttgcta acatggttaa acaacgcgca gcggctgatt
     6001 agcgatgttt cagcaaaacc ggtttatatc agtagttttt tcgccggaaa catgtcgccc
     6061 gatggctatc gccaactgct ggaacacgtt aaagcaaccg gcgttaatgt ctgggtacag
     6121 gatggcagcg gcgtggataa actgaccgct gaacagcgtg aacgttatct acaggccagc
     6181 gccgattgcc aaagatccgc cccagcaagc ggcattgttt atgaactgtt tgtcgccggc
     6241 aaaggcaaaa cctttacagc gaaaccgaaa ccggacgcag aaattgcctc gctgttagcg
     6301 aaacgttcct cttgcggtaa agacactctc tatttctctc tgcgctattt gcccgtcgcg
     6361 cacggcattc tcgagtatta aatctcctcc aggtaagtcg ggtacgacct ggcttacctc
     6421 tttcgctctc gtcagaattc catcaagatt aattgctaaa aagcgtgcta aagaaagaag
     6481 atcgttatcg gcgaacgggc cgatcactca tgcaggaagc atagatcctt tgagcggagt
     6541 ttctgatatc tgaggagtgc gaaatgcaat tgagcagcag tgaaccttgc gtggtgatcc
     6601 tgaccgaaaa agaggtagag gtaagcgtca ataaccatgc tacgtttacc cttccgaaaa
     6661 actacctggc cgccttcgcg tgcaacaata acgtcattga actctcaacg ttaaatcacg
     6721 tattaatcac ccacatcaac cgtaacatca tcaacgatta tctgttgttt ttaaataaga
     6781 acttaacctg tgtaaagccc tggtcgcggc tggctacccc ggttatcgct tgtcatagcc
     6841 gtacaccgga agtgttccgg ctagccgcca accacagcaa gcagcaaccc agcaaaccct
     6901 gcgaagcgga gttgacgcgc gcattgcttt ttaccgtatt gtctaacttt cttgagcaat
     6961 cgcggtttat tgccctactg atgtatatct tacgcagcag cgtccgcgac agcgtctgcc
     7021 gcattattca aagcgatatt cagcattact ggaatctgcg aattgtcgcc agttcgctat
     7081 gtttaagccc cagcctgctc aaaaagaaat taaaaaacga aaataccagc tatagccaga
     7141 ttgtcacaga gtgtcgtatg cgttacgccg tacagatgtt attgatggat aacaaaaata
     7201 tcactcaggt ggcgcaatta tgtggctata gcagcacgtc gtactttatc tctgttttta
     7261 aggcgtttta cggcctgaca ccgttgaatt atctcgccaa acagcggcaa aaagtgatgt
     7321 ggtgaagggc aaaccggaaa cggataagac gggcataaat gaagaagaaa tggcgcgccc
     7381 tgcaggattc gaacctgcgg cccacgactt agaaggtcgt tgctctatcc aactgagcta
     7441 agggcgcgtt gataccgcaa tgcggtgtaa tcgcgtgaat tatacggtca acccttgctg
     7501 agtcaatggc ttttgatctg gttgctgaac aagtgaacga ccgcgtctga ttttctgatt
     7561 tatttcgcta tagcggcaaa caaatgcaca ccgctgcgcg tctgaatcaa gaaaacccgt
     7621 attttcatgt atcaaagtac aatttaccgc cctaacggaa aattgtccgc tcctatgaga
     7681 ctggtaacta tgaaaccaac gtcggtgatc attatggata ctcatcctat catcagaatg
     7741 tctattgaag ttctgttgca aaaaaacagt gaattgcaga ttgtcctgaa aacggatgat
     7801 taccgcataa ccatcgatta tctccgaacc cgtcctgttg atttaatcat tatggatata
     7861 gacttgcccg gaacagacgg ttttaccttc ctgaaaagga tcaaacaaat ccagagcaca
     7921 gtgaaagtgt tatttttatc atcgaaatca gaatgctttt atgctggcag agcgatacaa
     7981 gctggtgcta acggttttgt cagtaaatgc aatgatcaga atgatatttt tcatgccgtt
     8041 cagatgatcc tctccggata cacttttttt cccagcgaaa cgctcaacta tataaaaagc
     8101 aataaatgta gtacgaatag ttcaacgatc actgtgctat ccaatcgtga agtgaccata
     8161 ttacgttatc tggttagcgg attatctaat aaagaaattg ccgataagtt attacttagc
     8221 aataaaacag ttagtgcgca taaatctaat atttatggca agctaggttt gcattcaatt
     8281 gtagagctta tcgactacgc caaattatac gaattaatat aatattaatt ataattgatc
     8341 ataaatatcg catccgcttt cgccacacct ggctgaacac cgctggctaa cgctcgataa
     8401 ttggcaaaaa agtttagtgt gacattgcca tttgcatcta cttcctcagt cgggctcgcc
     8461 tcccccagtg cgagccggga gcgatcgcta ttacgtaatt cgatggcaac ggtttgtgcc
     8521 attgcgggat catccagagc cagcaggttg gtatcggatg ccggcgttcc cgtaaataaa
     8581 atcgcaactg aacccggagg acatccctcc agccgcaggc taaaagggac gagtgccgtg
     8641 gtatcgccag cgttcagtaa ttgtgtcgta ggccatctgc ctaaatctac cgtcttatcc
     8701 ttatccgctg tgtttacggt acaagagaaa tcaaccacgt taccgtgcaa attgatatta
     8761 atcgttccta aagggtcagc tgcccatcca ctggaactcc acagtaaccc gcagaaacag
     8821 ctaaagaata ctcttctcat tctccgtacc tcattgataa tcgacgcgta aataccccag
     8881 cgcgctaaac ggcccctcgg tcggtttttg accggtaata ctgataggcc aggcgcgaag
     8941 tgtgacattg gctgccgcag ctgcatccag acggaaagga ataacgctat tgagatcgtt
     9001 aggcgtgatc ggcgtatcgt tctgatcggc gacaataaaa cctaaatcct gattgtccga
     9061 caccatcgcc tgaccagaaa cggcactggc ttccagacgc attgttaaat aagcctgcgc
     9121 agcaacattc gtacatttga ccgcaatgct cttggtttgc ggcatgacac cagcaggtcg
     9181 attacccggc cctgccgcac taaataacga tgcgccgata tcaccaaaat caaattcaac
     9241 aatctgcccg gcatttaatt cgcagttttg cggtacttca acccggccac caaaactaat
     9301 ggtataaaca ggtgtaacca acggatcgta aggcgttgat gtcacataca cggtaaacat
     9361 cacctgtctg gggatctcca ccatgttaat aaagggccga ataaccttta aacgaaatat
     9421 caaacgagaa tccatcaccc cgaacggaag gttttgcgaa acgttaggat ctgttcccat
     9481 acgaatataa ttttgcgggg gaaattgaat atccatcacg ctatcaacca gactcatcgc
     9541 acctaataga taatcattta attgcatata tttataatta tcgatagttt cctgaacaat
     9601 aaagttggtg acataactac ggtatgtata attcaccagc gtgccgggtg ggcaaattgc
     9661 tgacacacct acccagccgg atttttccgg taaaacaaca atctgccctg gctggttatt
     9721 ggtgctatta aagacatttg agagatcata gtgttcatct tctgctacgc cagtactatt
     9781 tgcacaaaca gtagcccagg atatgtttgc cagacacaaa catgccattg ccaataataa
     9841 tctgcagatt atcttcattg ataatttcct ttatgagcca ggatgtgtgc aaactgccga
     9901 aataacagtg acggcttgct gtaagctttg cttcggcaat acatagtgcg caatgcagcg
     9961 tgagttcgcg ccgtctcccc actgaaccag taattcacca gacaatggcg caccacttaa
    10021 ataaacttgc ccgtcatcac ccaccatact ggttattccg gtactgtttt cccgtaccag
    10081 tgatccaaac ggcaccggtt ttccgccctg ggtaacggta atgagcgccc gcacgcctat
    10141 gcgggtatca aaattggcac gaaccaacgc gccttgcgtc ggcacaacgc tgctaatatt
    10201 tttttcaaca tcgatggaat tccccatcgt attggtatca agcgcgatac ggttataccg
    10261 ataaaccgtg gcatacggca tcaccgcata gccgcgccag tcggttaaaa tgccagtttg
    10321 attttcaatg cgtacaccgc ctgcgccagg cgctttaatc aaaacattgg tatcccctaa
    10381 aggctggctc agcgttatgc cattttcatg cccgaccact ccgccagaca gctgccagtt
    10441 aacgtcatgt tgatcgcggt cgtagttata cccgactccc agcgtaccgt aagcggcctg
    10501 ccagtttgcc gtggcgctgc ccgtataccc attattcgag gtatcaccct ggcttacgtg
    10561 ataactcagg ttgtggcctt ccagtaaggt cccacctaca cctgccagcc agctattttt
    10621 cccgttgctg ttacggttgg cgttaaagga ggcataagcg cggtcgagcg cattttcccg
    10681 ggtgtagcga cgtttggtca aaacattgaa aggcactgaa acattaagtc cgacaatacg
    10741 ttcgttatcg gggatcccta cagattcatt ccacgaaaat gagagcgaat aactgatgcc
    10801 aacccagctg ctggtatacc ccacctgata ccacgtatct gaatccgaag tattccagta
    10861 tttttgatgg gtaccagaaa tataaagcga gccaaagtca ttaagtgatt gtgaaatatt
    10921 taactgcaaa cggtctttac ggctaaagcg taaattgtgg taattcacga ttatcggttc
    10981 atcccgatgc tcaccgtcgt aatcgtaatc atattcgtac ccctccattc gacgatacgc
    11041 aacatcatca agggtataaa aaccttgtgt cgaatagcgg taacccatta actgaaaatt
    11101 ggtgccgaag gtgttcatcg atttcgcata gaggaagcga atggaatccc cctcatgacg
    11161 actgtcgtcg gctaactggc tgcgcgcatg ggtcacatcc agcgacactg cgccccagtt
    11221 cccgaggttg cgccccagcc ctaataaaaa ggcggtgtaa ttggcagata attgcgtccc
    11281 gccgtaggca gtaaattcct gtggtaaacc gccgagtgcc gtgccctgaa aaaagaaagg
    11341 cgatgattgc tgactattac cgctacgaaa atcgcccgcc gtcaggtcaa atttgaaacg
    11401 cccttcgcgt tgtaaaattg gcactgttga atacggaatt gtgtaattct gctgattacc
    11461 atcgcgctcg tcgatggtga cgtccagatc gccatttgaa gatgtcgggt gcaaatctgt
    11521 aatttcaaaa gcgccggggg aaacatagct ttgatagata ataaaaccat tttgccgaat
    11581 cgttagctgg gccgccgtac gggcaatccc acgtaccgtt ggggcaaacc cttgctggct
    11641 atcaggatac atattatcgg aagaataaag ccgtacacca cgaaatccaa caccatcgaa
    11701 aatatcactt cctgtattgc cgtctcccat taccagttca cttttcagcg gaataatggc
    11761 gcgctgtacc caggtgccaa tattattcca ctgttctgaa tgatatccat ttccgcgaaa
    11821 atagttccag gaaccattgt tgcgtaaacg ccacgggcca atattaatcc cgctgagctc
    11881 actaaaaaaa taactatcgt taccattacc tctgttacca gtgaaattat aattcaggag
    11941 taaagcagga ataccttcat cccactcttc aggcggaatg taaccgtgag cgctactcaa
    12001 caacgctatc tgaggaatag tgatgttcag gcgcatcgct gcaaaatcaa agttaattgt
    12061 ggcatcaggt acagctttca gtaaattgat gcatttattg ttttgctgct gggctaactc
    12121 gggaaatgca ctgctattaa ggccaattcg ttcaaggagt acctggttaa aacagggcat
    12181 taacccacct gatttatcac ctgtattttc tgttgtcgat tcaaatacga tatcctgtga
    12241 accaatgaac tcatcattac gccagagatc tacccgatac acgcccgcag gttgatgatt
    12301 acctctctca aagcgcgata aatcagcaac ggatgtgcca ttctctaata aaaatgccgg
    12361 gttgaaataa ctttccgcat agcttaaaaa ggagggtaaa atggcaagag atgaataaca
    12421 aattccggcc agacaccagg tatacctttg cggaatatcc gtagtagtgg gtattttcat
    12481 aattagctct gatattgtca gatgattcgg ttagtttaag ttcacttctc tgaccggggt
    12541 tactgaacca taatcattaa cggtctgaaa tttcagcttt ccctgcactc ctgagggtaa
    12601 gggaatttgt gaattaactc tgggtgcagc catagcattc cccaactttt ggctgcccac
    12661 ttgtaagtta accagggtga cataaaaagg tgatggatta tgaacattga tatagttacc
    12721 ggatcgcgaa aacttgagtg tgtccggcgc ttctgcgggt aattcttgta attgaattgg
    12781 acggagaaat aatttcatgc gcgataaaat cgccagttgc aaaacattcc tgccgttcaa
    12841 tgcattttta tctaccgaag ggatcgtctt aacattcatc cagaacagag actcacgatc
    12901 tgctgccagc ggtggaccgg tgtaaataat acgcaaagta ttttcgcttt tgggttcact
    12961 aacaaatagt ggcggtgtaa tgatgaatga cttttctttt acaccgctgc tgttttcaat
    13021 ccacgaattg accagaaaac gctcattggt atggctattt ctaatccata ccgcagtctg
    13081 tttagcatca gcgggataaa taatacgcgt ggcacctaat gcgatccctc cggcagcatg
    13141 ggcgctggcc gaaa
//
LOCUS       DAZFKF010000126        12982 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFKF010000126 DAZFKF010000000
VERSION     DAZFKF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.128"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..843
                     /locus_tag="ACHKA1_004461"
     CDS             <1..843
                     /locus_tag="ACHKA1_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFO5682024.1"
                     /translation="AVSSQSTALPPRPLLQATQLPALVMTGKPGQSSVVYLREARQRS
                     VSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHRALNTLLTGQQLYGHMDWN
                     RKILTVTTTAAPPVDLTAPQGSQKTADTPRNPFRSTTGTAADQAQVPPAVSASRAQST
                     TGTSGTSAPRTTTPAPVVTKPVAPPPQKVTPPRQWKMEKGTTLKDGLARWASSEKCPH
                     GTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPVEKPLYARTSTAQCLLRVTD
                     RPQE"
     gene            848..1285
                     /gene="pilM"
                     /locus_tag="ACHKA1_004462"
     CDS             848..1285
                     /gene="pilM"
                     /locus_tag="ACHKA1_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFO5682025.1"
                     /translation="MGWFIVFIMVITTTGNFWFTSQLSRSEHRHQAAENTQQAATFIR
                     YMNAINDYLHQHPERRTAGGRLTSAQLGIPGTDTVSHIISQQRVFVWATETPGLMAAL
                     REQSNDSALLGRVENGRLLDTAGRALSITLPSVIPDHVILWMN"
     gene            1316..2935
                     /locus_tag="ACHKA1_004463"
     CDS             1316..2935
                     /locus_tag="ACHKA1_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFO5682026.1"
                     /translation="MNKKILAIMIAAALLQGCASYRQLESTRKEAVAEQQRAREMMSD
                     LRTRTPVVKESSRQWINPRPVMDKNTGKTVPGCPIVIHTRQSITLQQVVQRITESCHI
                     PVRITPDVLTYLNTSTTGSTQQIQGALPPPDASGMVPLAAIGASDTPPSRLQGNTAAL
                     SELNASGLTTLLNAVSSRLGISWRYDGSHIIFYYLETQTFPITYMDSQVAYNATVVSG
                     TMSSNGSSGGSASDAISGDASNTQTTTVDMKSALYQDVKNAVTSMLTPGIGRMFMSTG
                     FITVTDNPQVLETVRAFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDAITGGVSIVDGKLTGSKAFLKALSSQGDVSVVTQNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            2956..4251
                     /gene="pilO2"
                     /locus_tag="ACHKA1_004464"
     CDS             2956..4251
                     /gene="pilO2"
                     /locus_tag="ACHKA1_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFO5682027.1"
                     /translation="MTDISPTGRQVFPLTAGRNVFLAGLEWKTLPSQYRHARDFARAQ
                     KADLFLACQYLSNEDADTHTMVATVSRRILPGKPRQCFSLALLILPLLEHGGYAITEL
                     TLPGETPLYSFVSVVDGVLVSDLVGSGEEVREARDTFLSINTEPTQGWIHYEPAAFSA
                     GAQNQHLPLASLTGSGKHPAAARLNPVSRGPQFITVSLIIALLGTAWYGWQYYERWQT
                     EKAAQAAVARQATEERITPPWPGLPETDAFIRGCADIWTSLPLSAAGWRYSLAECSTD
                     GGSGNLRASYTNTAGATVTDFIRRISDMTDSRPFIVMPEGKTGGIGLPVTFRLSENAV
                     TVDALPETAVLQERLTSLAQSMQLKLDWQEVNNSLTDENGQIIQPPWKEYDLQMQTTL
                     PAHHLAERLKEPSVRFISVTRQLENGRFHYQFTGKYYAR"
     gene            4241..4693
                     /gene="pilP"
                     /locus_tag="ACHKA1_004465"
     CDS             4241..4693
                     /gene="pilP"
                     /locus_tag="ACHKA1_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO5682028.1"
                     /translation="MPDKAKTLLLSALLLPATAMATTGNAEQQNTQQIRIEQIQEETA
                     LYEAMLAREKIRHELRTLEQGVPAEGGRQQESISAVTTNAQALPSPRLTAIFGVQPQL
                     TARIELPGGSISDVKAGQNIPGTTYHVEKITPDSVVLEQSGTRKTLRP"
     gene            4778..6286
                     /locus_tag="ACHKA1_004466"
     CDS             4778..6286
                     /locus_tag="ACHKA1_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO5682029.1"
                     /translation="MLDPADLPPDDILDYVAIDTDNTGQLRIRVVEGKKHLRAVQEYL
                     TRLRARYQGRVGDFEFTTLDVIARLRQDATTTGDENAINPIQQKMLGYIRHSASLGAS
                     DLHMTPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQQIPTFSMLGYHPDQE
                     CTLRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTTRGWVDSIKSALRLDPDSILNGEIRDHDSAITAIKAAMTG
                     HLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRHPWNDV
                     ASRRTEDERHLVETFCNADAVYLRNHEGCPHCWRGVTGRTVIAEVISPDARFFQLYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGLVDPLAAHYISPIDEDSYTLLR"
     gene            6288..7388
                     /locus_tag="ACHKA1_004467"
     CDS             6288..7388
                     /locus_tag="ACHKA1_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO5682030.1"
                     /translation="MKFLEKLNSTELDLSALNSWTARKTFGLKRAEFYESLGGMIRDG
                     TPSLRALEFICNVETDFGTRKGTSGMYFLARECIASLKASGMLSGALPTWVPPEEAAL
                     IRNGEERGNIAEALFQVAKTVKARREMVSSLITLCLYPLLLLSLCVVNMYNAHTRIIP
                     IVSAFAPEEKWSFQMKVLAAMSELVIGYGLWLSGAIITLVIIIRFSLSRYTGPGRRLL
                     DKLPPWSLYQTFHGVNYLFSISSMLQINIPLSQALLRIEKSAEHNKWLKERIQAIRKN
                     VLSGQNLATAMRNSDYDFPSKICINKLLLNSEREDSVSAMNTYAGQWLEDAKKSVRKS
                     GLIITGISGGFVFFFILNMISAIYSISDMLKQ"
     gene            7462..7998
                     /locus_tag="ACHKA1_004468"
     CDS             7462..7998
                     /locus_tag="ACHKA1_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO5682031.1"
                     /translation="MNFRARLSSYFSLKKDKEDDKGLTLLEIAGAMIIIAIVVALAMS
                     QMSSTMASSDNQRELKNLQTIATKMKQYKFQGRYTGTDYIKTLTESGALPADMIAGGN
                     KAKNAWGGDVTIAATADKYGYIITSNNVPKTNCVELINSLRSSSIFTKIMNTAPNTVD
                     PVTVCSSDKNNIKLETNS"
     gene            8052..8525
                     /locus_tag="ACHKA1_004469"
     CDS             8052..8525
                     /locus_tag="ACHKA1_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO5682032.1"
                     /translation="MKWFYLSSLFLFCLPATAAVNSNTLDSCFIQAGKRYSIDPGLLK
                     VIARKESSLNPRAINHNRNASGKIISTDYGLMQINSTHIPGLKRMGIIRSENELLSNV
                     CLNIQTGAWILARHFQQCGVNWECLGSYNAGFNKNNTHRRMKYARLIYAAYMQGR"
     gene            8544..9179
                     /locus_tag="ACHKA1_004470"
     CDS             8544..9179
                     /locus_tag="ACHKA1_004470"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO5682033.1"
                     /translation="MFLIILLVISATLTIQLLAIRLAGHYLMQVHTLRRPRHLSLLCA
                     VLALTATITHIIRGTDITGLCFLLLMAGCMTLMTLTDAASCRLPRPFTLTFLMLGVAF
                     RLWQGDAAIALACALFWFLLLSLLRRYTSYRKGVETLGLGDVFLIAGVAMWSSANSTP
                     WIIGIAAASTMICMSLFPRSNTGAAIPFGPFICASLYIFTFFPGAIFHTGG"
     gene            9184..10548
                     /gene="pilV"
                     /locus_tag="ACHKA1_004471"
     CDS             9184..10548
                     /gene="pilV"
                     /locus_tag="ACHKA1_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFO5682034.1"
                     /translation="MQKDDDKGFVLFEILAGLIVIGIATPMIYSEIENWLNEQLYQSA
                     AYHADAYNTAARNYIADNNARLHSGSLPANFTADDLIRQGYLKQGFNHSPFGQSYITG
                     IRRNQTTGRLEALTCSTGGQTIKEEGLRSVAGQLPGLGGFISKNGTATGAFGAWTDKP
                     GDYGLTCSTGHIAVVMSGDDLQESDRLYRFQVAGRPELNQMHTAINMGGNNINNTGNI
                     NGQSATLKGDITSEDGWLITRNNKGWMNITHGGGFTMTDSQWIRAVNGKGITTTGEIK
                     GGKVSGGTVRSDGRLSTGEYLQLDKTATAGTKCSPDGLVGRTSTGAILSCQSGMWAGF
                     ESYPVGSPIPWPSATPPQGYLVMNGQSFSCSRYPQLARAYPGCKLPDLRGVFIRGWDN
                     GRGLDGDRNRHLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYI
                     VKAS"
     gene            11163..12329
                     /locus_tag="ACHKA1_004472"
     CDS             11163..12329
                     /locus_tag="ACHKA1_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO5682035.1"
                     /translation="MFQKKTRKMSLSRALDKYLKTVSVHKKGHLQEFYRVNVIKRHPM
                     ADRYMDEITTIDIAGYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGTC
                     RMNPVELVRKPKISNGRDRRLTSGEERRLSRYFRDKNPQLYVIFHLALETAMRQGEIL
                     SLRWEHLDLQHGVAHLPETKNGSPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFKS
                     AWRTALLDLKIENLHFHDLRHEAISRLFELGTLNVMEIAAISGHRSLNMLKRYTHLRA
                     YHLVSKLDARRKQTSKISPYFVPYPATVRNRNGVFIVTLHDFDLESRAETRELAISHA
                     SVLLLRALAQAAQRGERVPTPGELPANIDERVMICPLTNSQFMTKANSIFISSS"
     gene            12371..12661
                     /locus_tag="ACHKA1_004473"
     CDS             12371..12661
                     /locus_tag="ACHKA1_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682036.1"
                     /translation="MKHLSSRAATADNNRIVTIEDVYTIAFSQPIHTESALGQRWMLI
                     HTGEYESASDAKRFLDTLVTGHCACMVTPSPVLDHDTWLRIRLLMGEIRDAK"
     gene            complement(12753..>12982)
                     /locus_tag="ACHKA1_004474"
     CDS             complement(12753..>12982)
                     /locus_tag="ACHKA1_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682037.1"
                     /translation="AMVPVVLVKPWDSMTFIFMGAVCVSFAVILFWLVYIVCLYIGTL
                     VKNIFIRIFAFSIWALSAEGAIIYNMIKILS"
BASE COUNT         3388 a         3564 c         3135 g         2895 t
ORIGIN      
        1 gcagtcagca gccagagtac cgcactgcct ccccgcccgc tccttcaggc cacacagctg
       61 cccgcactgg ttatgaccgg taaacccggt cagtcatccg tcgtttattt acgtgaggca
      121 cgtcagcgct ccgtcagtga ctggatacgc cagttgctgc cacccggctg gcagttccgg
      181 caacagaacg ctgccatacc ggctctgaac aaacgcctcc ccgtctggtc cgttaatgac
      241 cagtggcacc gggcactgaa tactctgctg accgggcagc aactgtacgg acatatggac
      301 tggaacagga aaatactgac tgtcacgacc actgcagcac cgcctgttga cctcacggca
      361 ccgcagggtt cacagaaaac agcggacaca ccacgtaatc cgttcaggag cacaaccgga
      421 acggcagcag accaggcaca ggtaccacct gccgtcagcg cttcccgggc tcagagcaca
      481 acagggacat ccggtacatc tgcgccccgc acaaccacgc cagccccggt ggtcacgaaa
      541 ccggtagcgc caccaccaca gaaggtcaca ccgccacggc agtggaaaat ggaaaaaggg
      601 accacgctga aggacggact ggcccgctgg gcgtcgtccg aaaaatgccc ccacggcacc
      661 tggacgctac gctgggaaac gcccgttaac tacagtatcg atgcccctct ggttttcacc
      721 ggcagtttcc gccaggcgct ggaacagctc ttcgcactct acaccccggt ggaaaaaccg
      781 ctgtatgccc ggacaagcac agcacaatgt ctgctgcggg tgacagatcg tccacaggaa
      841 taaccttatg ggctggttca tcgtgttcat catggtcatc acgaccacag gcaatttctg
      901 gttcaccagt cagctctccc gcagcgaaca ccgtcatcag gccgcagaaa acacacaaca
      961 ggcagcaacg ttcattcgtt atatgaacgc catcaatgat tacctgcatc agcatccgga
     1021 acgacggaca gccggagggc ggctgacatc cgctcagctc gggattcccg ggacggatac
     1081 cgtcagtcat atcatcagcc agcaacgtgt ttttgtctgg gccacagaaa ccccgggcct
     1141 gatggccgcg ctcagggaac agagtaatga ctctgctctg ctcggcaggg tggaaaacgg
     1201 ccgactgctg gacactgccg gcagagcgct ttccatcaca cttccgtcag tgatccccga
     1261 tcacgtcatc ctctggatga attagtcacg aatctgaata aatcacggat ctcccatgaa
     1321 taaaaaaatt cttgccatta tgattgccgc cgctctgtta cagggctgtg ccagttacag
     1381 acagcttgag agcacaagaa aggaagccgt tgcggaacaa caacgggcac gggaaatgat
     1441 gtctgatctg cgaacccgga cgccggttgt taaggaatcc tcacgacaat ggatcaatcc
     1501 ccgtccggtc atggataaaa acaccggcaa aaccgttccc ggttgtccca ttgtcattca
     1561 tacccgtcag agcatcaccc ttcagcaggt ggttcagcgg atcacggaaa gctgccatat
     1621 tccggtacgg atcacacctg atgtactgac atacctgaat acatccacaa ccggcagcac
     1681 acaacaaatc cagggcgcgt tacccccacc ggatgcgagc ggcatggttc cccttgccgc
     1741 cattggcgca tctgacacgc caccatcccg tctgcaggga aataccgcag cactgtctga
     1801 actgaatgcc tccggcctga cgaccctgct gaatgcggtc tcctcccgtc tcggtatatc
     1861 ctggcgctat gacggctcgc atatcatctt ctattatctc gagacacaga ccttccccat
     1921 cacctatatg gacagtcagg tggcctataa cgccactgtg gtcagcggca ccatgtcctc
     1981 aaacggctca tcaggaggca gtgcatcaga tgccatttcc ggtgatgcca gcaataccca
     2041 gaccaccacc gtggatatga agtctgcgct gtatcaggat gtgaaaaatg cggtcacatc
     2101 aatgctgacc ccgggtatcg gacgcatgtt catgtccacg ggctttatca ccgtcacaga
     2161 taatccacag gttctggaaa cggtccgggc attcattgaa aaacgcaatg aagagatgaa
     2221 acgtcaggtg gtgctgaatg tggaaattct gagtatccgg aaaacccgca atgagcaggc
     2281 cggtatcgac tggaacgcag tcttcagcga cagaacgctg ggactgagtc tcggcagcac
     2341 attcaccagt gcagccagtg atgccatcac aggtggtgtt tccattgtgg acggtaaact
     2401 caccggttca aaagccttcc tgaaagcgtt gtcttcccag ggggatgtca gtgtggtgac
     2461 tcagaactct gccgtcacca aaaacctgac gccggtgcca atgcagatcg ccaatcagca
     2521 gagttacatc gagagtgtga cgacggatac cactgccaat gtcggctcat ccacctccct
     2581 gaatgcggcc accatcacca ccggttttaa catgaccctt ctgccgttca ttctcccgga
     2641 ctcacagaca ctgcagttgc tctattccat gagtctctcc gacaaaccgg ttattgagaa
     2701 ttatgagagc ggtggaagta aagcccagct gccgaatgtg gatctgaaaa ccatcaatca
     2761 gaccgtggat ctgaaatccg ggcagacggt cattatcagt ggcttccagc agtcaggacg
     2821 tcgcagcgga aaacagggtg taggcacgcc gggattcttc ggtcttggcg gtgggatcaa
     2881 cagtgaaaac gacgacacca ttctcgtggt gctgatcacg cccaatatca tctgatttgc
     2941 acaaggagga cgatcgtgac ggatatttca ccgacagggc ggcaggtgtt tccgctgacg
     3001 gcagggcgca atgtgtttct ggccggtctg gaatggaaaa cactgccctc acagtaccgg
     3061 catgcccgcg actttgcccg ggcacagaaa gccgaccttt tcctggcctg tcagtatctc
     3121 agtaatgaag atgccgatac ccacaccatg gtagccactg tcagtcgccg catactgccc
     3181 ggaaaacccc gtcagtgctt ctccctcgcc ctgctgatcc tgccactact tgaacatggc
     3241 ggatatgcca tcacggaact gacgttaccc ggagaaacac cgctctatag ctttgtttcc
     3301 gttgtcgacg gtgttctcgt cagtgacctg gtgggaagcg gggaagaggt tcgggaagcc
     3361 cgggatacct ttctttccat caacaccgaa ccgactcagg gctggataca ctatgaacca
     3421 gcggcatttt cagcaggcgc tcagaatcag cacctgccgc tggcctccct gactggctct
     3481 ggtaaacatc cggctgccgc acgcctgaat ccggtatccc ggggaccgca gttcatcact
     3541 gtctctctga tcatcgccct tctcggcact gcatggtacg gctggcagta ttatgaacgc
     3601 tggcagacag aaaaagccgc gcaggcagcg gttgcccgac aggccacaga ggaacgaatc
     3661 actcccccct ggcccggact ccctgaaaca gacgcgttca tccggggatg tgctgatata
     3721 tggacttcac tgccactgtc tgctgccgga tggcgttatt cgctggcaga atgctcgact
     3781 gacggtggca gcggcaatct tcgcgcatcg tacaccaata ctgcgggcgc gacagtgact
     3841 gacttcatcc ggcgcatcag tgatatgaca gacagccgac cgtttattgt gatgccggaa
     3901 ggaaagaccg gcggcattgg cctgccggtc accttccggc tgtctgaaaa cgccgtcaca
     3961 gttgatgcac taccggaaac cgctgtcctg caggaacgac tgacctcgct ggctcagtca
     4021 atgcaactga aactggactg gcaggaagtt aataacagcc tcactgatga gaacggacag
     4081 atcattcagc ccccatggaa agagtatgac cttcagatgc agaccacact ccctgcacac
     4141 catctggctg aacgcctgaa agagccttct gtccgtttta tctctgtcac acggcaactg
     4201 gagaacggtc gtttccacta tcaatttaca ggtaaatatt atgcccgata aagccaaaac
     4261 ccttctgctt tcagccctgc tgttaccggc aacggcaatg gccacaaccg gaaatgcaga
     4321 gcaacagaac acacagcaaa taaggattga acagatacag gaagagacag ccctgtatga
     4381 agccatgctg gcgagagaga agatccgcca tgaactgcga acacttgagc agggcgttcc
     4441 cgcagagggc ggacgtcagc aggaaagcat ttccgcagtt acaacaaatg cacaggcatt
     4501 accatccccc cgcctgacag caatattcgg tgttcagcct caactgactg ccaggataga
     4561 acttccgggg ggaagtatct ccgatgtgaa ggcgggccag aacatccccg gaaccactta
     4621 tcacgtggaa aaaatcacgc cggacagtgt agtgctggaa cagtctggca ccagaaaaac
     4681 cctgcgacca tgagactggc cgccgtaact ggcggctgtc aaaaataaaa ggatgaaaga
     4741 catattttcc gggcaccgct gcccgtaagg aggctttatg ctggatccag cagatctgcc
     4801 gccggatgat attctggatt atgtggccat cgacaccgac aacaccggac aactgcggat
     4861 cagagttgtg gaggggaaaa aacatctgcg tgccgtccag gaatatctga cccgccttcg
     4921 cgcacgctat caggggcgtg tgggagactt tgagttcacc acgctggatg tgatagcccg
     4981 cctcagacag gacgccacaa cgacaggcga tgaaaacgct attaacccga tacagcagaa
     5041 aatgctcggc tatatccgcc attctgcctc ccttggtgcc tccgaccttc atatgacgcc
     5101 agggcgggac aacacagact tcacctatgt ggaagcccgg gttcatggcg aactggaagt
     5161 gctcgatatc ctccggaaag aagaaggtct ggaattactg ggggctacct attccggcat
     5221 gacggatgtc atcaagggca cacaatttga tcccggggtt ccacaggacg cacgtctctc
     5281 tgaacagtac ctgaaactgg ctggcctgtt tggcgcacgc tactcccact acccctgcgt
     5341 gggaggcctc tatgctgttc tccgtctgat caaggatgac agccagcaaa tcccgacctt
     5401 cagtatgctg ggttatcacc cggatcagga gtgcaccctg cgccgtatcc tgcaacgccc
     5461 tgaaggcatt atcaccctct ctggccccac cggctcggga aaatccacca cgctgagaac
     5521 cgcctctgcg gcataccttg aacaatacgg cttcaacaac accggcggta ttcttctgcc
     5581 acggcgcagg ttgttcacga ttgaatcccc gccggaaggc cgtatccccg gagcaattca
     5641 gacagccgtt atggatacca cccggggctg ggtggactcc atcaaatcag cactgcgtct
     5701 tgatcctgac agcatcctga acggtgaaat ccgcgatcac gattccgcca ttacggcaat
     5761 aaaagccgcc atgaccgggc atctgatgct gaccaccatc catgcgaacg acccgattaa
     5821 catcctcgaa cgccttgaga tggagggtgt tcaggccagg atgattgcag acccgcagtt
     5881 gttcatcgga ctgctcagtc agcgtctggt tcagctgata tgtccccact gtcgtcatcc
     5941 atggaatgac gtagcatccc gtcgtactga ggatgagcga catctggtgg aaacattctg
     6001 taatgctgat gcggtatatc tgagaaacca tgaaggctgt ccacactgct ggcgtggtgt
     6061 cacagggcgt acagtgattg cagaagtcat ctctcctgac gccagatttt ttcagttata
     6121 ccgggaaaaa ggccgtatcg aagctaaaac ctactggcac agggaactgg gcgggatgac
     6181 gcgtaatcag catctgctgg gaaaaatcaa cagcggactg gttgaccctc tggcggccca
     6241 ttacatctct cccattgatg aggacagcta tacgctgctg cgctgatatg aaatttctgg
     6301 aaaaactcaa cagtactgaa ctggatctgt ctgccctgaa cagctggaca gccagaaaaa
     6361 ccttcggtct taagcgtgcc gagttctatg agtccctcgg cggaatgata cgtgacggaa
     6421 cgccgtcact gcgcgccctg gagtttatct gtaatgtcga aactgacttt ggcacccgga
     6481 aaggaacatc cggcatgtac tttctggcca gagaatgtat tgcctccctg aaagccagcg
     6541 gtatgctgtc gggggccctg cccacatggg ttcccccgga agaagcggca ctgatcagga
     6601 acggtgaaga acgcgggaac atcgccgaag ccctgttcca ggtcgcaaaa accgtgaaag
     6661 cacggcggga aatggtgagc agtctcatca cactctgtct ttatcccctt ctgctgcttt
     6721 cactctgtgt ggtgaatatg tacaacgcac atacccgcat catccccatt gtcagcgctt
     6781 ttgcaccgga ggagaaatgg tcattccaga tgaaagtcct tgcagccatg tcagagcttg
     6841 tcatcggcta tggtctgtgg ctcagtggtg ccatcatcac gcttgtcatc atcatccgct
     6901 tctcactcag tcgctatacc ggcccgggac gcaggcttct tgacaaactg ccaccatggt
     6961 cgctctatca gacttttcat ggtgtgaatt atctgttcag catatcgtca atgctgcaga
     7021 taaatattcc gctttcacag gcgctgctca gaattgaaaa aagtgcagag cataataaat
     7081 ggttaaagga gagaattcag gcaataagaa aaaatgtatt atccggccag aatctggcca
     7141 ccgccatgcg caattctgac tatgattttc cttcaaaaat atgcatcaat aaacttctct
     7201 taaattctga aagggaagac agcgtatctg caatgaatac ctatgccgga cagtggctgg
     7261 aagatgcaaa aaagagcgtc agaaaaagtg gattaattat caccggtatt tcaggaggtt
     7321 ttgtattctt ttttattctt aacatgatta gcgcgatata cagtatcagc gatatgctca
     7381 agcaataata cctgccgtaa cactaaaacg cccttcaata tactttcatt aacgtaacaa
     7441 ccattaatac aggagtcata tatgaacttc agagcacgtc tgtcttctta tttttcttta
     7501 aaaaaagata aagaagatga taaaggttta acattattag aaattgccgg cgcaatgatt
     7561 atcatcgcaa tagttgtcgc gctcgcgatg agccagatgt ccagcacaat ggcctccagt
     7621 gacaaccagc gcgaactgaa aaacctgcag acaattgcaa ccaaaatgaa gcaatacaag
     7681 ttccagggaa gatataccgg aacggattat atcaaaaccc tgacggaaag cggtgcattg
     7741 cctgctgata tgattgcagg aggcaacaaa gccaaaaacg catggggtgg tgatgtcacg
     7801 attgctgcca cagcagacaa atatggctac attatcacca gtaataatgt tcctaaaaca
     7861 aactgtgttg agctgattaa cagcctgcgc agttcgtcca tatttaccaa aattatgaac
     7921 acagccccga acacggtgga tcccgttacc gtctgcagta gcgataaaaa caatatcaag
     7981 ctggaaacca acagctaatg ccctgccaca cagcactccc tccggggtgc tgtttcccgg
     8041 agcacataat catgaaatgg ttttatctct catctctttt cttattctgt ctcccggcaa
     8101 ctgccgccgt aaacagtaac acactggata gctgctttat tcaggcagga aaacgttact
     8161 ccatcgatcc cggactactg aaagtaattg cccgtaaaga aagcagcctg aatccccggg
     8221 ccatcaatca taacagaaat gccagcggaa aaattatcag cacagattat ggcctgatgc
     8281 agattaattc cacacatatt cccggactga aaagaatggg aattatccga agtgaaaatg
     8341 aactgctcag taatgtttgc ctgaatatac agacaggtgc atggatcctt gcccggcatt
     8401 ttcagcagtg tggtgtgaac tgggagtgtc tcggtagcta caacgccgga tttaataaaa
     8461 ataacactca ccgcagaatg aaatatgccc ggctgattta tgccgcatat atgcagggac
     8521 gttaataacg gagaatacga gccatgttcc tcatcatctt actcgtgatc tccgctacgc
     8581 tgaccataca gttacttgcc atcagactgg ccggacatta tctcatgcag gtgcataccc
     8641 tccggcgtcc acgtcacctc tccctgctct gtgctgttct ggcactcacc gccaccatta
     8701 cgcacatcat acgggggaca gacattaccg gactttgctt tctgttgctg atggccggct
     8761 gcatgacact gatgacactg actgatgcag cctcctgccg tcttccccgt cccttcaccc
     8821 tcacattcct gatgctgggt gtggcgtttc gtttatggca gggcgatgct gctattgcgc
     8881 ttgcctgtgc tctgttctgg tttctcctgt tatctctcct gaggcgctac acctcatacc
     8941 gcaagggagt ggagacgctg ggactggggg acgtgtttct gattgcaggt gtggccatgt
     9001 ggtcatctgc caacagcact ccgtggatca tcggtattgc cgcagccagc acaatgatct
     9061 gtatgtcgct gttcccgcgc agtaatactg gggccgctat tccgttcggt ccgtttatct
     9121 gtgcatcact ctacattttc actttttttc ctggtgccat ttttcacacc ggagggtaac
     9181 accatgcaaa aagacgacga taaaggtttt gttctgtttg aaattctggc cggactgatt
     9241 gtgatcggta tcgccacacc aatgatttat tcagaaattg aaaactggct gaatgagcag
     9301 ctttaccaga gtgctgcata tcatgcagat gcctacaata ccgcagccag aaactatatt
     9361 gccgacaaca atgcccgtct gcatagcggc agccttccgg caaatttcac ggctgatgac
     9421 ctgattcgtc agggatatct gaaacagggg tttaaccatt ctccctttgg tcagagttat
     9481 atcaccggta tccgccgtaa ccagaccacc gggcgtctgg aagccctgac ctgctcaact
     9541 ggagggcaga caatcaaaga agaagggctg cgtagtgtgg cgggtcagtt gccggggctg
     9601 ggtggattta tcagcaaaaa tggaacggca acaggcgcat tcggagcctg gacagataaa
     9661 ccgggagact acggacttac ctgttcgaca ggtcacatcg ccgtcgtcat gagcggtgat
     9721 gacctgcagg aaagtgaccg tctctaccgt tttcaggtag caggtcgtcc tgaactgaat
     9781 cagatgcata ccgcaatcaa tatgggcggg aataacataa acaacaccgg caatatcaat
     9841 ggtcagagtg ccacactgaa aggtgatatt acgagtgaag acggctggct aatcaccaga
     9901 aacaataagg gatggatgaa tatcacacat ggtggtggat tcaccatgac ggacagccag
     9961 tggatacgcg cggttaatgg caaaggcatc acaaccaccg gggaaatcaa aggggggaaa
    10021 gtcagtggtg gtacagtccg gtctgacggt cgtctttcaa ccggggagta tttacagctt
    10081 gataaaacag ctacggcagg cacaaagtgt tcaccggacg gacttgtagg ccgcaccagt
    10141 acaggggcca tactttcgtg ccaatccggt atgtgggcag gttttgaatc ctaccctgtc
    10201 ggaagcccta taccgtggcc atcagccacc cctccacaag gctacctggt gatgaacggg
    10261 cagtcatttt catgttcccg gtatcctcag cttgcccgtg cttatcccgg gtgtaagctg
    10321 ccggatttgc gcggtgtttt tatccgtggc tgggacaacg gacgtgggct ggatggagat
    10381 cggaacagac atctcctgag ttatcaggcc gatcaatccg gtgtctatca tgagcgtggg
    10441 ggatggctga aagggcatca cagcggtatg ccttactggg cccagggaag cacaacggaa
    10501 atgcgcccca aaaacattgc ctttaactac atcgtcaaag caagctgatg catttaaggc
    10561 gtattaactt ttaacttaca gcaacgtatt ttgacccaga ccatgcttcc cgaatacccc
    10621 ggtttacctc tgtacgctct gaatcctgcc gcaccatagc ctgctttgcc gtcacagacg
    10681 gcaaagccgt ttgagccact accccagttc gcataaaccg gttttctgga gtcttcttct
    10741 gcgtcataac aggccgaata atcaaaaacc agtgcatcgc tgatattatt cttccagaca
    10801 ccggattggc acgctggcta ctgcctgacg cttaattaaa taccggcccc aggtatattg
    10861 agacacgtaa cagatatcgt tccacctgac tcataattac cccacgcata attattgaca
    10921 taccagacat tatttttatc ggaataaacc tgctgaaaac caccgaaagg agaaccatta
    10981 agatacgtcc atgcacaata tgcatggaca ccaagtctga aattacgaat attttttccg
    11041 atgttatagg tctgcgttgt gaatttaatt tcagttgacc gccagacacc ggattggcac
    11101 gaaagtattg catttctgta tccagcctcc taccttcccc gtttttatcg tggaaggcag
    11161 atatgtttca aaaaaaaact cgcaaaatgt ccctcagccg ggcactggac aaatacctga
    11221 agactgtttc cgtccataaa aaaggtcatc tgcaggagtt ttatcgcgtc aatgtgatta
    11281 aacgtcaccc tatggccgat cgctatatgg atgaaatcac taccattgat atcgccggtt
    11341 accgggatca gcgtttagct caaattaacc cacgaacagg gcgtcaaatc accggaaata
    11401 ccgtccgtct tgaacttgcc cttttatcat cattattcaa tatcgcccgt gtggaatggg
    11461 gaacatgccg tatgaatcct gttgaactgg tccgaaaacc gaaaatcagt aatggccgcg
    11521 accgccgact gacttctggt gaagaacgcc gcctttcccg ctatttcagg gacaaaaatc
    11581 cgcagctcta cgttatcttc catctggctc tggaaacagc aatgcgccag ggagaaattc
    11641 tctcattacg ctgggaacac ctcgatttgc agcacggcgt ggcgcatctt cctgaaacga
    11701 aaaatggctc accacgcgac gtaccactct cccggaaagc acgtaactac ctgcaaattc
    11761 tgccacaaca gataaacggc aatgtgttca gttacacctc gtccggtttc aaaagcgcct
    11821 ggcgtaccgc tcttctggat ctgaaaatag aaaatctgca ttttcacgat ctccgccatg
    11881 aggccatcag ccgtctgttc gagctcggca cactgaacgt tatggagatt gcagccattt
    11941 ccggtcaccg ctccctgaac atgttgaaac ggtacacaca tctccgggca tatcacctgg
    12001 tcagtaaact cgatgcaagg cgtaaacaga ccagcaaaat ttccccttat tttgttccct
    12061 accctgcaac tgtcagaaac agaaacggag tgttcattgt gacactgcac gatttcgacc
    12121 ttgaaagccg tgctgaaacc cgggaactgg caatatctca cgccagcgta ttgttgctgc
    12181 gcgcactggc ccaggctgct cagcggggtg aacgcgtccc cacaccaggc gagctacccg
    12241 caaacattga tgaacgggtc atgatttgtc cgttaacaaa cagccagttc atgacaaaag
    12301 cgaacagtat tttcatatcc tcctcctgaa caaataattt ttgttcaaaa ttaacgctat
    12361 gagacccttt atgaaacact tatcctcccg tgcagccact gcggataata atcgtatcgt
    12421 tacaattgaa gacgtttaca caatcgcatt ctcgcaacca attcacaccg aatcagcgtt
    12481 ggggcaaagg tggatgttga tccatactgg tgaatatgaa agtgccagcg atgcaaaacg
    12541 cttccttgat acgctcgtca ccgggcactg tgcttgcatg gtaacaccat cacctgttct
    12601 ggatcatgac acctggctgc gtatccgcct gctgatgggc gagataaggg atgccaaata
    12661 aacattcatg gccccaacac ataaaaataa cgccggggac tctggcagag gtaccattct
    12721 ttttacatag agccaatata taaacaactt ttctatgata atatctttat catgttataa
    12781 ataattgccc cctcagcaga aagggcccaa atagaaaaag caaaaatcct tataaaaata
    12841 ttctttacaa gcgttcctat atacaaacaa actatataaa ccagccagaa cagaatgact
    12901 gcaaaagaaa cacacacggc ccccataaaa ataaatgtca tcgaatccca tggtttcacc
    12961 agtacaaccg gaaccatggc tg
//
LOCUS       DAZFKF010000127        12922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFKF010000127 DAZFKF010000000
VERSION     DAZFKF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.129"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..234)
                     /locus_tag="ACHKA1_004475"
     CDS             complement(<1..234)
                     /locus_tag="ACHKA1_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO5682038.1"
                     /translation="MRYNGLNNMFFPLCLINDNHSVTSPSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKMDDHEKVIGLIQKMKRIYDSLPSG"
     gene            complement(412..2061)
                     /gene="actP"
                     /locus_tag="ACHKA1_004476"
     CDS             complement(412..2061)
                     /gene="actP"
                     /locus_tag="ACHKA1_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO5682039.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGCWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(2058..2372)
                     /gene="yjcH"
                     /locus_tag="ACHKA1_004477"
     CDS             complement(2058..2372)
                     /gene="yjcH"
                     /locus_tag="ACHKA1_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO5682040.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2505..4463)
                     /gene="acs"
                     /locus_tag="ACHKA1_004478"
     CDS             complement(2505..4463)
                     /gene="acs"
                     /locus_tag="ACHKA1_004478"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO5682041.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4856..6292
                     /gene="nrfA"
                     /locus_tag="ACHKA1_004479"
     CDS             4856..6292
                     /gene="nrfA"
                     /locus_tag="ACHKA1_004479"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO5682042.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6337..6903
                     /gene="nrfB"
                     /locus_tag="ACHKA1_004480"
     CDS             6337..6903
                     /gene="nrfB"
                     /locus_tag="ACHKA1_004480"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO5682043.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6900..7571
                     /gene="nrfC"
                     /locus_tag="ACHKA1_004481"
     CDS             6900..7571
                     /gene="nrfC"
                     /locus_tag="ACHKA1_004481"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO5682044.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7568..8524
                     /gene="nrfD"
                     /locus_tag="ACHKA1_004482"
     CDS             7568..8524
                     /gene="nrfD"
                     /locus_tag="ACHKA1_004482"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO5682045.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8544..10262
                     /gene="nrfE"
                     /locus_tag="ACHKA1_004483"
     CDS             8544..10262
                     /gene="nrfE"
                     /locus_tag="ACHKA1_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO5682046.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFVLISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGVVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGPDRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10255..10638
                     /gene="nrfF"
                     /locus_tag="ACHKA1_004484"
     CDS             10255..10638
                     /gene="nrfF"
                     /locus_tag="ACHKA1_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO5682047.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10635..11231
                     /gene="nrfG"
                     /locus_tag="ACHKA1_004485"
     CDS             10635..11231
                     /gene="nrfG"
                     /locus_tag="ACHKA1_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO5682048.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11573..12886
                     /gene="gltP"
                     /locus_tag="ACHKA1_004486"
     CDS             11573..12886
                     /gene="gltP"
                     /locus_tag="ACHKA1_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO5682049.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
BASE COUNT         2905 a         3318 c         3574 g         3125 t
ORIGIN      
        1 tcctgatggt aaactatcat aaattctttt cattttctga atcaagccta tcactttttc
       61 atgatcatcc attttgaaaa gagagagggc tttattgtca attaacgtgt ttttatgatg
      121 tttgctgtaa ttatctgatt ttgttttctt tgtatgtgat ggacttgtga cagagtggtt
      181 atcgttaatc aggcaaagag ggaaaaacat attatttaaa ccattatagc gcataaaata
      241 tccttataat taacaagaaa gaaaaggcat attctctgca ttatcatttt ctacgactgt
      301 caaaaatcgc tcatttttta atgagtttat ttgtttaata ttatgggaaa aggtgatgca
      361 tttgggagag gaagagtatt ccccggtcag acgaccgggg aaggggagag attaatgcgc
      421 gcggccttgc tcaacgccaa agccggtctg ggagcggata aactgcgcac ggaacagttc
      481 acgctcgcgc gcaccttccg ctgagttatc ggttgccgag aagaaccaga tgccgaggaa
      541 tgccacgctg atagagaaca gcgccgggta ttcatacggg aagatggctt tttcgtgacc
      601 gaggatctgt acccaaatcg tcgggccgag gatcatcagt accactgcgg taattagccc
      661 cagccagcag cccatcattg cgccacgcgt agtcagtttc gaccagtaca tagaaagcag
      721 aatgatcggg aagttacagc tcgccgcgat ggcaaacgcc agccccacca taaaggcgat
      781 gttctgattc tcaaacagca cgccgaggat aatcgcaatc acacccaaaa tcaggacggt
      841 gattttcgat acccgcagct cttcacgttc ggtcgcgcct tttttgaaga cgttagcgta
      901 caggtcatgc gaaaccgctg atgcgcccgc cagcgtcaga cccgcaacca ccgcgaggat
      961 agtggcgaaa gcaaccgctg aaataaaacc gaggaacagg ttgccgccca ctgcattcgc
     1021 caggtgaacg gccgccatgt tgttaccacc aatcagatgg cccgccgcgt ctttatattc
     1081 cggatttgca ccgaccagca tgatcgcgcc gaagccgata ataaaggtca ggatatagaa
     1141 gtagcccata aatccggtag cgtagaacac gctcttacgc gcttcgcggg catcgctgac
     1201 cgtaaagaag cgcatcagaa tgtgcggcaa gccagccgta ccaaacatca atccaagccc
     1261 cagcgacagc gcggagatcg gatctttcac cagccctccc ggcttcatga tgtcgacacc
     1321 tttcgggtgt accgccatcg cttcgctgaa cagattgttg aagctaaagc cgacgtgttt
     1381 catcaccata aaggccataa aactggcacc gaacagcaac agcacagctt tgataatttg
     1441 tacccaagtg gtcgccagca tgccgccgaa caggacgtac atcatcatca gcacgccgac
     1501 cagcaccact gcaatgtgat agttaaggcc aaacagcagc tcgatcagtt tacctgcgcc
     1561 caccatctgg gcgataaggt aaagcgccac caccaccaga gaaccacagg ccgaaagaat
     1621 acgaatcggc ccttgtttca gacgataaga ggctacatcg gcaaaggtgt aacgccccag
     1681 gttacgcaga cgttcggcaa tcaggaacaa aatgatcggc cagcccacca ggaagcccag
     1741 cgagtaaatt aagccgtcat agccggaggt aaacaccagt gcggagatcc ccaaaaatga
     1801 ggcggcggac atatagtcac cggcaatcgc cagcccgttc tgaaaaccgg tgatattgcc
     1861 gcctgcggtg taatagtcat tacgcgaacg tacgcgtttt gacgcccagt aggtaatgcc
     1921 gagcgtaaac acgacgaaaa tcaggaacat aataatcgcc tgccagttcg ttggctggcg
     1981 ctctacggcc ccgctaatag catccgcggc gttagctgcg aaagggagtg tggcggcaag
     2041 cgccgtcaga actcttttca tgatgcttgt acctcatgca ggacttcgtt attaagacgg
     2101 tcgaattcgc cgttcgcccg ccagatgtag ataccagtga gaacaaagga gatcactatt
     2161 accccaacac cgactggaat accgcgtgtg acgctggtgt tcggattcag cggggtgccc
     2221 agccagccgg gcgcgaaggc gatcagtaaa ataaagccga tataaactgc cagcataata
     2281 atcgacagga tggtggcaaa ccgttgccgt ttttcgacta actccctgaa atgcgcattg
     2341 tcttctatcc gctgataaat cgtaccattc atcacagatt ctccagaggt aatgtaggga
     2401 ttgttttaat tcccgctccc ttatgggaga aggttaacgc tcgggtaacc cttgccgaat
     2461 gtaggccgga taaggcgtta tacgccgcat ccggcaattg tgggttacga tggcatcgcg
     2521 atagcctgct tctcttcaag cagcttctcg actacgccag gatcggcaag cgtcgaggta
     2581 tcgcccaggt tgctggtatc gcccgccgca attttgcgca gaatacggcg cataattttg
     2641 ccggagcggg ttttaggcag ggagtcggtc cagtgcagca cgtctggcgt cgccagcggg
     2701 ccaatctctt tacgcaccca gttgcggact tctgcgtaca gttctggtga cggttcctcc
     2761 ccgtgattaa gcgtgacgta ggcgtagatc gcctgacctt taatattgtg cggaatacct
     2821 actacggcgg cttcggcaat cttcggatgt gccaccagcg ccgactctat ctccgccgtc
     2881 cccagacggt gaccagagac gttcagcacg tcgtccacgc gcccggttat ccagtaatag
     2941 ccatcttcat cgcgacgcgc gccgtcgccg ctgaaataca tatttttgaa ggtggaaaaa
     3001 taggtctgct caaaacgttc gtgatcgcca aacagcgtac gcgcctgacc cggccaggag
     3061 tcggtgatca ccagactgcc ttcggtggcc ccttccagcg ggttaccttc gttatcgacc
     3121 agcgccggtt gcacgccgaa gaacggacgt gttgccgaac cggctttcag ctcggtagcg
     3181 ccaggcagcg gggtgatcat gaaaccgccg gtttcggtct gccaccaggt atcgaccacc
     3241 ggacatttct cgttgccgat ttttttccag taccactccc acgcttccgg gttaattggc
     3301 tcgcccacgg aaccgagaat gcgcagcgac gaacggtcgg tgccttcgat cgctttatcg
     3361 ccttccgcca tcagcgcgcg aatcgccgtg ggcgcggtat agagaatatt gacctgatgc
     3421 ttgtccacca cctgtgccat acgggcaggc gtcggccagt tcggtacgcc ttcaaacatc
     3481 agcgtggtcg cgccgcaggc cagcgggccg tacagcaaat aactgtgtcc ggtaacccaa
     3541 cccacatcgg cggtgcacca gtagatatcg cccggatgat aatcaaagac atatttaaag
     3601 gtcagcgccg cgtacaccag ataaccgccg gtagtgtgca gtacgccttt tggttttccg
     3661 gtagaaccgg aggtatagag aataaacagc ggatcttcgg cgttcatctc ttccgcctgg
     3721 tgctgatcgc tggcttgctc aaccaggtcg tgccaccaca ggtcgcgccc ttcctgccag
     3781 tcaatttttc cgccagtacg cttcagtacc accacatgct ctacgctggt gacgttcggg
     3841 ttttttagtg cgtcatcaac gtttttcttc agcggaatac tacgcccggc gcgcacgcct
     3901 tcgtcggaag tgatcaccag tcgtgagttg gaatcgataa tgcgcccggc aaccgcttcc
     3961 ggcgagaagc caccgaaaat taccgaatgc accgcgccaa tacgggcgca ggccagcatc
     4021 gcaaccgcgg cttccggcac catcggcata taaatcgcca ccacatcacc ttttttaatg
     4081 cccagctcga gcagggtatt agcgaagcgg cagacgtcgc ggtgcagctc tttatagctg
     4141 atatgtttgc tctggctggc gtcgtcgcct tcccagatga tggcggtacg atcgccgttt
     4201 tcttgcagat ggcggtcaag gcagtttgcc gccagattca gcgtgccgtc ctcgtaccat
     4261 ttaatggaca cattaccggg ggcaaaggag gtgtttttca ccttctggta cggtttgatc
     4321 cagtcgagaa tttttccctg ttcgccccag aaggtatcag gtgcgttaat agattgttga
     4381 tacatcgcct cgtactgctg agggtttatc aggcaacggt ctgcgatgtt ggcaggaatg
     4441 gtgtgtttgt gaatttgact catgcttttg ttctccttgt aggatgttaa taatatgtgg
     4501 cataagcgtt aaatgtaggg gtattggcag ttttgtttag tatttgggcg acagatcacg
     4561 caaaagtaga attgtgcaaa taaacggcag ggtaattttt gaaggtcagc aacaaaagtt
     4621 gattaattct ttgaggaaca tgcagttatg catgctgttg aaaaagagga agatactgac
     4681 taactctaaa gtggtatttt acatgcactt acaattgatt aaagacaaca ttcacagtgt
     4741 ggttatttgt tacacatagg ggcgagcaat gtcatgacag tgtaggtgcg gttactgtcg
     4801 tgaaaaacaa taaaaaccgc cattgcaaca atggcgcaat tcggatgaag cccctatgac
     4861 aaggataaaa ataaacgcac gccgtatctt cagcttattg attccttttt tctttttcac
     4921 ttctgttcac gctgaacaaa cggctgctcc cgcaaaacct gtaactgtgg aagcgaagaa
     4981 tgaaaccttt gccccgcagc atcccgatca atatctctcc tggaaagcca cctcggaaca
     5041 gtcagagcgt gttgacgccc tggcggaaga tccacggctg gtgatcctgt gggcggggta
     5101 tcccttctcg cgcgattaca acaagccgcg tggacatgct tttgctgtga ccgatgtgcg
     5161 tgaaaccctg cgtaccggtg cgccgaaaaa cgctgaagat ggtccgctac cgatggcgtg
     5221 ctggagttgt aaaagcccgg atgtggcgcg tctgatccag aaagacggcg aagatggcta
     5281 cttccacggt aaatgggcgc gcggcggccc ggaaattgtc aacaacttag gttgtgccga
     5341 ttgccataac accgcctcac cagagttcgc caaaggcaaa ccggagttaa ccctttcccg
     5401 tccgtatgcg gctcgcgcga tggaagccat tggtaaacct tttgagaaag ccggacgttt
     5461 cgaccagcaa tcgatggttt gcggtcagtg ccatgtggag tattacttcg acggcaaaaa
     5521 caaagcggtt aaattcccgt gggatgacgg catgaaagtc gaaaatatgg agcagtatta
     5581 cgacaaaatt gccttctctg actggactaa ctccctgtcg aaaacgccaa tgctgaaagc
     5641 gcagcacccg gaatatgaaa cctggacagc gggcattcac ggtaaaaaca acgtgacctg
     5701 tatcgactgc catatgccaa aagtgcagaa cgccgaaggc aaactctaca ccgaccataa
     5761 aattggtaat ccgtttgata acttcgccca gacttgtgcg aactgccata cccaggacaa
     5821 agctgccttg caaaaagtgg tcgcggaacg taagcagtcg attaacgacc tgaaaatcaa
     5881 ggttgaagat caactggttc acgctcactt cgaagcgaaa gcagcgctgg atgcaggcgc
     5941 gacggaagcc gaaatgaagc caattcagga cgatatccgt catgcccagt ggcgctggga
     6001 tctggcgatc gcttcccacg gcattcatat gcacgcaccg gaagaaggtt tacggatgct
     6061 cggtacggcg atggataaag cggcggatgc acgcaccaaa ctggcgcgcc tgctggcgac
     6121 caaaggcatc acccatgaaa tccagatccc ggatatctcg accaaagaga aagcccagca
     6181 ggccattggc ctgaacatgg aacaaatcaa ggccgagaag caggacttca tcaaaacggt
     6241 gatcccgcag tgggaagagc aggcacgtaa aaacggtctg ttaagccaat aaccccgttc
     6301 cgcctcgcaa ggggcggaaa acacaatgga gtgaatatga gcgtattacg ttcgttatta
     6361 actgccgggg tgctggcgtc gggcctgttg tggagcctga acggaattac cgctacccct
     6421 gcggcgcagg catctgatga tcgctacgaa gttacccagc agcgtaaccc ggatgccgcc
     6481 tgtctggact gtcataagcc agataccgaa ggtatgcatg gcaaacatgc ttccgttatc
     6541 aacccgaata acaaactgcc ggtcacctgc accaactgtc acggccagcc atcaccgcaa
     6601 caccgcgaag gggtgaaaga tgtgatgcgc tttaacgagc cgatgtacaa ggttggagag
     6661 cagaacagcg tctgtatgtc ctgccatctg ccggaacagt tgcaaaaagc gttctggccg
     6721 cacgatgtcc acgtaaccaa agtggcgtgc gccagctgcc attccctgca tccacagcaa
     6781 gatacgatgc agacgttaag tgacaaagga cggattaaga tttgcgtcga ttgccacagc
     6841 gatcagcgca ccaatccgaa ctttaacccg gcgtctgttc cgttgcttaa ggagcagcca
     6901 tgacctggtc tcgtcgccag tttctcaccg gcgtcggcgt gctggcagcc gtcagcggca
     6961 ccgcagggcg cgtggtggcg aaaacgttga atatcaatgg ggtgcgttac ggcatggtgc
     7021 atgacgagtc gttatgcatc ggctgtaccg cctgtatgga tgcttgtcgg gaagtgaaca
     7081 aggtgccgga aggcgtctcg cgcctgacga ttattcgtag cgagccgcag ggcgaatttc
     7141 ctgatgtgaa atatcgcttc ttccgtaagt cttgccagca ctgcgatcat gcgccgtgcg
     7201 ttgacgtctg cccgaccggt gcgtcttttc gtgatgccgc tagcgggatt gtcgatgtta
     7261 atcctgatct ctgcgtcggt tgtcagtact gcatcgccgc ctgtccgtac cgcgtgcgct
     7321 ttatccatcc ggtcacgaag acggcggaca aatgcgattt ctgccgtaag accaatttgc
     7381 aggccggtaa gctgcccgcg tgcgttgagg cttgcccgac gaaggcgctg acgtttggca
     7441 atctggacga tcctaacagt gagatttcgc aactgctgcg ccagaagcct acttaccgct
     7501 acaagctggc gctgggaacc aaaccgaaac tgtaccgcgt accgtttaaa tacggggagg
     7561 tgagccaatg acgcagactt ccgcatttca ttttgaatcg ctggtgtggg actggccgat
     7621 tgccatctac ctgtttttga ttggtatttc tgctggtttg gtgacgctgg ctgtgctgtt
     7681 acgtcgcttc tacccgcagg cgggcggtgc agacagcacg ttgctgcgca ccacgctgat
     7741 tgtcggacct ggcgcggtga tcctcggtct gttgatcctc gtcttccacc tgacaagacc
     7801 gtggaccttc tggaagctga tgttccacta cagttttacc tcggtgatgt cgatgggggt
     7861 gatgctgttt cagctttaca tggtggtgct ggtgctgtgg ctggcgaaaa tctttgaaca
     7921 tgatttgctt gccctgcaac aacgctggtt gccgaagctg gggatcgtgc aaaaggttct
     7981 gagcctgctg acgcccgttc atcgcggact ggaaacattg atgctggtgc tggcggtgtt
     8041 gttgggggct tataccggtt ttctgctgtc ggcgctgaaa tcgtatccgt tccttaataa
     8101 cccgatcctg ccggtgctgt tcctcttctc cggcatctcg tccggtgcgg cggtggcact
     8161 gatcgccatg gcgatacgcc aacgcagtaa cccgcattcc acggaagcgc agtttgtaca
     8221 ccgtatggaa atccccgtgg tatggggtga aatcttcctg ctggtggcgt tttttgtcgg
     8281 tctggccttg ggcgatgacg gtaaagtgcg tgcgctggtg gcggcattgg gtggcggttt
     8341 ctggacgtgg tggttctggc ttggtgtcgc cgggctgggg ctgattgtgc caatgttgct
     8401 caaaccgtgg gtcaatcgca gttccggcat tcctgccgtg ctggcggcgt gtggagccag
     8461 cctggtcggc gtgttgatgt tgcgcttttt cattctctac gccgggcagt taacggtggc
     8521 gtaagccaga acagaggtgg tttctggacg tattccttcc tgaagtcggt tttctggcgc
     8581 tgttgttaag tctcggggtc aacgtgttga ccccgttgac ggcctttgcg ggagtgcggt
     8641 tgcgctggcc tgccatgatg cgactcactt gcatcggcat tctggcacag ttcgcgctcc
     8701 tgctgctcgc ctttggcgta ctgacgtatt gttttctcat cagcgatttc tcggtcattt
     8761 atgtcgccca acatagctac agcctgctgt cgtgggaact caaactggcg gcggtgtggg
     8821 gcggtcatga aggttcgctg ctgctttggg tgctgctgct ttccgcctgg agcgcgctgt
     8881 ttgcctggca ttatcggcag caaaccgatc cgctatttcc gctgacgtta gccgttttat
     8941 ctctcatgct cgccgcactg ctactgtttg tagtgctgtg gtccgatccc ttcgtgcgga
     9001 tatttccacc agcaatcgaa ggccgcgatc tcaatccgat gctgcaacat cccggtctta
     9061 tctttcatcc accgctgctt taccttggct atggcggttt gatggtagcg gcgagcgtgg
     9121 cgctggcgag tctactgcgc ggcgagtttg atggtgcctg cgcccgaatt tgctggcgct
     9181 gggcgttacc tggctggagt gcattaactg cggggatcat cctcggttcc tggtgggcct
     9241 attgcgaact gggctggggc ggctggtggt tctgggatcc ggtggaaaac gcctctttac
     9301 taccctggct ttctgccact gcgttgctgc acagtttatc cctgacacgc cagcggggga
     9361 tttttcgcca ctggtcgctg ttactggcga tagttactct gatgctgtcg ctgctgggca
     9421 ccttaattgt ccgttctggc attctggttt cggttcatgc gttcgcgctg gataacgtcc
     9481 gcgccgtgcc gttgttcagt ctgtttgtac tgattagcct tgcgtctctg gctctgtatg
     9541 gctggcgagc gcgggacggt ggcccggcgg tgcgtttttc ggggttatcg cgggaaatgt
     9601 taatcctcgc tacgctgttg ctgttttgcg cagtgctact gatcgtgctg gtgggaacgc
     9661 tttatccgat gatttacggc ctgctgggct ggggacgcct ctccgttggc gcaccgtatt
     9721 ttaaccgcgc gacgttaccg tttggtctgt tgatgttggt ggtgattgtg ctggcgacgt
     9781 ttgtctctgg caaacgcgtg cagcttccgg cgctggtagc tcatgcgggc gtgctgttat
     9841 ttgccgctgg ggtcgtggtt tccagcgtca gccgtcagga aatcagcctg aatttacagc
     9901 cgggtcagca ggtgacgctg gcaggataca ccttccgttt tgagcgcctc gatctgcaag
     9961 ccagaggcaa ttacaccagc gaaaaagcga tagtggcact gtttgaccat cagcaacgta
    10021 ttggtgaact gacgccggag cggcgttttt atgaagcacg ccgtcagcaa atgatggaac
    10081 cgtcaattcg ctggaacggc atccatgact ggtatgcggt catgggggag aaaactgggc
    10141 cggatcgtta cgcttttcgt ttgtatgtac aaagcggtgt gcgctggatc tgggggggag
    10201 gattgttgat gattgcgggc gcattgttaa gcggatggcg ggggaggaag cgcgatgaat
    10261 aaaggacttc tcacgctact gctgttattt acctgttttg cgcgcgctca ggttgtagac
    10321 acctggcaat tcgccaatcc gcaacaacag caacaggcgt taaatattgc cagccagtta
    10381 cgttgtccgc agtgccagaa tcaaaactta ctggaatcca acgcgccggt ggcagtcagt
    10441 atgcgccatc aggtttacag catggtggcg gaggggaaaa gtgaagtcga aatcattggc
    10501 tggatgaccg aacgctacgg agattttgtt cgctataacc caccgttaac gggtcagacg
    10561 ctggtgttgt gggcgctgcc agtggtgttg ttactgctga tggcactgat cctctggcga
    10621 gtgagggcga agcgatgaaa cagcctcaaa taccggtgaa aatgctgaca acactcacga
    10681 tattgatggt attcctctgt gtcggcagtt atctgttaag cccaaaatgg caggcggtac
    10741 gtgcggagta tcagcgtcag cgcgatccgc tacatcagtt tgccagccag caaaccccgg
    10801 aagcgcagct tcaggcattg caggataaaa tccgcgctaa tccgcaaaac agcgaacagt
    10861 gggcgttact gggcgagtac tatctgtggc aaaacgatta cagcaattcg ctgctggcgt
    10921 accgtcaggc gttgcaacta cgtggtgaga acgctgaact gtatgcggcg ctggcgacgg
    10981 tgctttatta ccaggccagc cagcatatga ccgcccagac tcgcgcaatg atcgacaaag
    11041 ccctcgcgct ggactctaac gaaatcaccg ctctgatgct gctggcttcc gatgcgttta
    11101 tgcaggcgaa ttacgcgcaa gccatcgaac tatggcaaaa agtgatggat ctcaactcac
    11161 cccggatcaa ccggacacag ctggtcgagt cgattaatat ggcgaaattg ttgcagcgga
    11221 gatcggatta atcatctggt gatgaagatt atgttattga gctgcataaa aaataatcga
    11281 atgaacatat accaaaaata atcaccaatc agtattattg cagattaaac aaataaaaat
    11341 ctttccataa caaatggtta ttcattaatc ctgccataaa ctcgcgttat tatgcattaa
    11401 tgcagcgaaa agctctgttg ttaaagggtt gcgcaacata ccgcgcaaat gatactgatc
    11461 ataagcgtta aaaaaatcta caaaccaacg caacacaatt catgccctgg cagtatgtca
    11521 cgttctcgcg tttctgaacg gggaacggcg ctccattgag gaagttattc tgatgaaaaa
    11581 tataaaattc agcctggcct ggcagattct gtttgctatg gtgctgggca ttctcctggg
    11641 aagctacctg cactaccata gcgacagccg cgactggctg gtcgtcaatt tgctctctcc
    11701 ggcgggtgat atcttcatcc atctgatcaa aatgattgtt gtgccgattg tgatctccac
    11761 gctggtggtg ggtatcgcgg gtgttggtga tgccaaacag ctcgggcgta ttggcgcgaa
    11821 aaccattatc tacttcgagg tgatcaccac cgtcgccatc attttgggga tcactctggc
    11881 gaacgtcttc cagcccggtg ccggggtgga tatgtcgcag ttggcgaccg tcgatatctc
    11941 gaaatatcag agcactacgg aagcggtaca aagcagttcc cacggcatta tgggcacgat
    12001 tttgtcgctg gtgccgacga atattgtggc gtcgatggcg aaaggcgaaa tgctgccgat
    12061 catcttcttc tcggtgctgt ttggtctggg gctttcctcc ctgcccgcga cgcatcgtga
    12121 accgctggtg accgtgttcc gctccatctc tgaaaccatg tttaaagtga ctcacatggt
    12181 gatgcgttat gcgccggtgg gtgtgtttgc gctgattgcg gtgacggtgg ctaactttgg
    12241 tttctcttct ctgtggccac tggcgaaact ggtgctgctg gtgcatttcg ccattctgtt
    12301 cttcgcgctg gtagtgctgg gaattgtggc gcgcctgtgc gggttaagcg tctggatcct
    12361 gattcgtatt ctgaaagatg agctgattct ggcgtactcc actgccagct ctgaaagcgt
    12421 actgccgcga attattgaga agatggaagc ctacggagca ccggcgtcga tcaccagctt
    12481 cgtggtgccg accggttact cttttaacct tgatggttcg acgctgtatc aaagtattgc
    12541 cgctattttc atcgctcagc tgtatggcat tgacctgtcc atctggcagg aaatcattct
    12601 ggtgctgacg ctgatggtga cctcgaaagg gattgctggc gtgccgggcg tgtcgtttgt
    12661 ggtgttgctg gcaacgctgg gtagcgtagg tattccgctg gaaggtctgg cgtttattgc
    12721 tggtgttgac cgtatcctcg acatggcgcg tactgcgctg aacgtggtgg gtaatgcgct
    12781 ggcggtgctg gtgattgcca agtgggaaca caaatttgac cgcaaaaagg cgctggctta
    12841 tgagcgtgaa gtgctgggca aatttgataa aactgcggat caataattga agattgccgg
    12901 ggatatcccc ccggcaatgt gt
//
LOCUS       DAZFKF010000128        12806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFKF010000128 DAZFKF010000000
VERSION     DAZFKF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.130"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(99..564)
                     /locus_tag="ACHKA1_004487"
                     /pseudo
     CDS             complement(99..564)
                     /locus_tag="ACHKA1_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545887.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(1223..1405)
                     /locus_tag="ACHKA1_004488"
     CDS             complement(1223..1405)
                     /locus_tag="ACHKA1_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682050.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICLLQKIKIIDKTKYK"
     gene            1344..3434
                     /gene="iha"
                     /locus_tag="ACHKA1_004489"
     CDS             1344..3434
                     /gene="iha"
                     /locus_tag="ACHKA1_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO5682051.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGMNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLFSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(3652..3802)
                     /locus_tag="ACHKA1_004490"
                     /pseudo
     CDS             complement(3652..3802)
                     /locus_tag="ACHKA1_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            4295..4537
                     /locus_tag="ACHKA1_004491"
     CDS             4295..4537
                     /locus_tag="ACHKA1_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682052.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     VTCDIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            4828..5196
                     /locus_tag="ACHKA1_004492"
     CDS             4828..5196
                     /locus_tag="ACHKA1_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682053.1"
                     /translation="MKDNNTTATPEVWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIPPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHKKDEV"
     gene            5200..5415
                     /locus_tag="ACHKA1_004493"
     CDS             5200..5415
                     /locus_tag="ACHKA1_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682054.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            5412..5684
                     /locus_tag="ACHKA1_004494"
     CDS             5412..5684
                     /locus_tag="ACHKA1_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neurotensin receptor R8"
                     /protein_id="HFO5682055.1"
                     /translation="MTDITDRHTLRRMSWSELFTAAQEAEFQRDYERARIVWSFALHV
                     ATTTINKNLAIAHIRRCDTLLHKSKTVPGNNTGGRSVCLRPQHPRR"
     gene            complement(5993..6824)
                     /locus_tag="ACHKA1_004495"
                     /pseudo
     CDS             complement(5993..6824)
                     /locus_tag="ACHKA1_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            6882..7067
                     /locus_tag="ACHKA1_004496"
                     /pseudo
     CDS             6882..7067
                     /locus_tag="ACHKA1_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(7531..7683)
                     /locus_tag="ACHKA1_004497"
                     /pseudo
     CDS             complement(7531..7683)
                     /locus_tag="ACHKA1_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
     gene            8020..9576
                     /locus_tag="ACHKA1_004498"
     CDS             8020..9576
                     /locus_tag="ACHKA1_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO5682056.1"
                     /translation="MHDYLLFLLGLTPLLAMIILILKVKMPIHYSVLSTLLLTLILGE
                     VFWQTPVMDMGSAVVYGAIKGLWPIVIVILAAIYSYNLMNATKSMDILRNVLASVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLALFNILLPFVIVTIIGGGMKAIRGVGLITL
                     MCGISTLIPQYLVAVNLGAELPAFAGSLVSLIVIVFMAKNRKEKTEPEFLIESSADSA
                     SALSYSRGALLRACSIYILIFLFILLCSPLFPTIKSAVSQIASSLTFSLSNGSNLSMK
                     IEWIATPGVLIIIASVIGGLIQKASLSCLLNVFVSTVKQLKNSIIAISAIVAMATVMD
                     VCGLIGTLAQSLVDLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHV
                     DPLWLAAANTSGATGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLK
                     VGLVYHLFMA"
     gene            9626..10345
                     /locus_tag="ACHKA1_004499"
     CDS             9626..10345
                     /locus_tag="ACHKA1_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO5682057.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQKSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNLISSLEENDWPVVSPAGSCTYTIKNYARYLSEEPEWAARAE
                     KVAERLHDLTSFIVNHLGITDVGAKLNGRAVYHPSCSLSRKLGVTREPLLLLENVQGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVGHIMDVKPDFLIGADASCLLNIGG
                     RLNREGHPVKVMHIAEVLMHR"
     gene            10356..11771
                     /locus_tag="ACHKA1_004500"
     CDS             10356..11771
                     /locus_tag="ACHKA1_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO5682058.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSRAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREDATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNAVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLHEHLGYDGPETPEAMTLFIREKIRQDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFQEVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEILGSEFSDVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFKDLPYACSLCTACNGVCPVKIPLATLIQKHRQVMAES
                     EITPLTERRITRAFNYINSHPALWKVGMVAGAKTARLFIRKGKTPISVGAIKEWTEAR
                     DLPSPDGESFRSWFKRHKKEG"
     gene            11775..12473
                     /locus_tag="ACHKA1_004501"
     CDS             11775..12473
                     /locus_tag="ACHKA1_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO5682059.1"
                     /translation="MENREEFLVAIARKLGRPVRHIPEAMPEPVNTLATTRLTELTSD
                     QRCEAFIKFASEVMLAECVLVSPENAPSTALDICWKFGSGPVIISNDQRLVDTGITPL
                     LQKEMDAALWDPEKGDENIHLAEKAKTGVVFAEYGLTESGGIVLFSAPERGRSVSLLP
                     EASLFVLRKSTILPRVAQLAQKLHDMAQEGQRMPSCINLIGGPSSTADIELIKVVGVH
                     GPVHATYLIIDDCD"
BASE COUNT         3493 a         2875 c         3043 g         3395 t
ORIGIN      
        1 ttcataattt agagaatgtt ttgcaataat atttggcagt attcagaaat acctgaaaaa
       61 tcatactata cagccccagg gaatggatga agattctaac aaagcatttc aacaatatat
      121 gcttgttaaa aatccatatc gaaatgccgt tgcagcattc atatatgcct cattcataaa
      181 atgtaaaaga gcaagctcat accagtaggg ggaaaattca ttgacatgtc ctgtcaatac
      241 gtacagagcc ctgtcatgct tgccagcacg taacaattcc gcccccagta accagcgtgg
      301 cccctgatta ttctcagggt tatatgttag tattttatca atgagtataa cggcatcctg
      361 atgacgctgt aaatgaacat tcgccagatg agcgtgaata tcaatgatgt caggagcatg
      421 tcaggcaaga cgcctgagtt tgttgacgta atttgtttcg tttatttcat cactatcgta
      481 tgcgtcaaga gttttttcaa actcatcccg aaatgggcca aaccaggttt caggaaataa
      541 gaaatacccc tggttattgt tcacttaaaa gtataatttg taattcaaat cctgagcctg
      601 taaaacgggg agtaacatat tctattctga agagaataaa agtcgtgatg cgatgtatca
      661 agcccggatt gtaatcccag attaacatag atcacaaaac aacttattct tcactaacgt
      721 caagataaat atcgttgtat gctctatcac gactacgaat accagcaaga tacaactgac
      781 atacggaaag ataccacgtt ttttactccc gaaaataacg ctaaaaagct acttccccat
      841 cgtttgtcct tagtattgtc agcgccaaca atgtgggcag acatgataaa gctgtctagg
      901 aaatagttcg cctcctcagc ggacaatcca aatggtgatt gtctctgtta aacgtttatt
      961 ttgaaggtcg actgaataag gtgatgacgc tgtagaattt ttcacgtgcc acagaatttt
     1021 gaacgctttc tcttacaata tttcaatgtt tctatcagta ttcgccggaa gaagtcatcg
     1081 accaaatcat cccagtcgtc tcgcaccact gaccattcat ggttgacatg tggtggaaat
     1141 ccgcttctac agtaaccatt ttttactcgc aaaaccgaac acgccatacg gatagctgtt
     1201 aactggcatg ccgaggcagt cgttatttat atttagtttt gtcaataatc tttatttttt
     1261 gtaaaaggca aatataaatt attctcatta ttgtttgtat ttgtgtattg tcttgccggt
     1321 taacatgatc ggagattagt aatatgcgaa taaccactct ggcttccgta gtcattccct
     1381 gtctcggatt ttcagccagc agcatagctg ctgcagagga tgtgatgatt gtctcggcat
     1441 ccggctatga gaaaaagctg actaacgcag ccgccagtgt ttctgtgatt agccaggagg
     1501 aattgcagtc cagccagtac cacgatctgg cggaggctct gagatcagta gagggtgtgg
     1561 atgttgaaag tggtacgggt aaaaccggtg ggctggaaat cagcatccga ggaatgccag
     1621 ccagttacac gctgatactg attgatggtg ttcgtcaggg cggaagcagt gacgtgactc
     1681 ccaacggttt ttctgccatg aataccgggt tcatgccccc tctggccgcc attgagcgta
     1741 ttgaggttat cagggggccg atgtccacac tgtatggctc tgatgcgatg ggcggtgtgg
     1801 tgaatatcat taccagaaag aatgcagaca aatggctctc ttccgtcaat gcagggctga
     1861 atctgcagga aagcaacaaa tggggtaaca gcagccagtt taatttctgg agcagtggtc
     1921 cccttgtgga tgattctgtc agcctgcagg tacgcggtag cacacaacag cgtcagggct
     1981 catcggtcac atcactgagc gatacagcag ccacgcgtat tccttatccc acggagtcac
     2041 agaattataa tcttggtgca cgtcttgact ggaaggcgtc ggagcaggat gtgctctggt
     2101 ttgatatgga taccacccgg cagcgttatg ataaccggga tgggcaactg gggagtctga
     2161 cggggggata tgaccggacc ctgcgctatg agcgaaacaa aatttcagct ggctatgatc
     2221 atactttcac cttcggaaca tggaaatcgt atctgaactg gaacgagaca gaaaataaag
     2281 gtcgtgagct cgtacgcagt gtactgaagc gcgacaaatg ggggcttgcc ggtcagccgc
     2341 gggagcttaa ggaatcgaac cttatcctga attcattact gcttacccct ctgggagaat
     2401 ctcatctggt tacggtgggg ggcgagtttc agagctcgtc catgaaagac ggagttgtcc
     2461 ttgccagcac aggtgaaact ttccggcaga aaagctggtc ggtatttgct gaggatgagt
     2521 ggcatctcac ggatgcactt gcgctgactg cgggcagccg ctatgaacat catgagcaat
     2581 tcgggggaca cttcagtccg cgtgcatatc tggtctggga tgtggcagat gcctggacgc
     2641 tgaaaggcgg tgtgaccacg ggatataagg cacccagaat ggggcagcta cataaaggga
     2701 ttagtggtgt gtccgggcag ggaaaaacaa atctacttgg taaccccgac ctgaagccgg
     2761 aagagagcgt cagttatgag gctggggtgt attacgataa ccccgccggt atgaatgcca
     2821 atgtcacagg ttttatgact gacttctcca acaagattgt ctcttattcc ataaatgata
     2881 acaccaatag ctatgtaaac agcggaaagg cccggttgca cggtgtggaa tttgccggca
     2941 cattgccgct gtggtcagag gatgtcacgc tgtcactgaa ttacacctgg acccgaagtg
     3001 aacaacgtga tggtgataac aaaggtgcgc cgctgagtta tacccctgaa cacatggtga
     3061 atgcgaaact gaactggcag atcaccgaag aggtggcatc atggctgggt gcccgttatc
     3121 gcgggaaaac accacgtttc acccagaatt attcgtcact gagcgctgta cagaagaaag
     3181 tgtatgatga gaaaggagaa tacctgaaag cctggacggt ggtggatgca ggtctgtcgt
     3241 ggaagatgac ggatgccctg acgctgaatg ctgcggtgaa taacctgctc aacaaggatt
     3301 acagtgacgt gagcctgttc agtgccggta agagtacgct gtatgccggt gattacttcc
     3361 agacgggatc atcaacaaca ggatatgtga tacctgagcg aaattactgg atgtcgctga
     3421 actatcagtt ctgataataa caaaacgcta tcactgacgg tagaatacgt tgccactgca
     3481 actcctggcg gaacagtggc aacgtattag gttaagtgca tttccgatcc gctaatgaga
     3541 tttcgttacc aacaactaat atcgtcacag gaaatgcacg gattattttt aacttatcat
     3601 ttacatactt gtccagagtg taagcgcacc gtgaaggacg aggggtaaaa attagtttac
     3661 agagagagtg acgttccagg gcaacaactc tttcatgcgg ttggcaggcc aggtgttgat
     3721 tacactgatc acgtggcgta gccacgtttc cggctcgatt ccgttaagtt ttgcagctac
     3781 cgatcaggct gtacatcact gccgcactat cgctcgtcat ctcaaagtcc ggtccgtcag
     3841 caggaaggta tcattctctc ccgccatttt tccgggggcc cggtcagata agtccctttg
     3901 tctatcgctg actcctggct cataacccgg ttagcagaat gcagggtcac cacctcgcca
     3961 cgaccgagtc cggataagtc aattgcacct tctcaatcgc cattttgtca caattaagtt
     4021 tgataaagca ggtcacctct gcactgaatc tgctccagtg cctcattggt ctccgtcccc
     4081 ccctttaaac tgccggataa gactgctgca cagcgccttc cccggaatca aatcactttc
     4141 cctgtccgcc acaatccgct tcatgccacc tcgtattttg tttcagagac cggaatctgc
     4201 cagaaagtga ctacgcgtgc acatatcggt tttctgggtt acatgcatcg tgttattttc
     4261 tgaaatgcaa aataataaaa aacaaggaca cataatgaag gatgaaatac acagtaccag
     4321 catcaacccg gatgttgttg agcagtttct ggcccttatg aacacacatt gtaaatcctc
     4381 agaagaggcg ctcgccatgt tgagtacgct ttgttacaac ctgatggtga catgtgatat
     4441 ccattccatg gacagccaga tgctcaatgg gcatgggata tccatgcaac ttcaggggat
     4501 tgatattgcc gaaaggccag gtaagaatat tcactaacct gaacagggtc tgaaaacgct
     4561 gtcttttcag gcctctgcag gcaaaaactg atgacaacaa aatttctgca tttgtacccc
     4621 accgatgaaa aagacaaaaa ctcacaccgg acttctagcc agtaaagata aaacccgacg
     4681 tgtcagcctc tacgaaacgc caacggcatg gtgtatcagg gggcaggagt gctacagcaa
     4741 aagtacagga cgccgctgtg gcagccacga cagtctgagc agactgcgcc tggacagcat
     4801 taagcctgtt gaatgagtgg tggcaccatg aaagataaca acacaaccgc caccccagaa
     4861 gtctggaaaa tgcgttttct ggccaacggg acatccggca catggtttta cactgacgac
     4921 attgcagtca ctgacgcgtg gcttcgcgcg cacccggatg atgtggaggt tatccctctc
     4981 tgccggatac caccccgtca gcattcccat gaacaacata ttgaagaact ccgcgcagcc
     5041 ctggcccgga tgctccatat gcatcatatg atgctggaaa ccacgcatta cagggaaaac
     5101 atcaccgatg cccgttgtat ccgtgaaatg catgaagctc cattacaggc gctcaatatt
     5161 ctggccagat acccgcataa aaaggacgag gtgtgacgca tgtggctact gattttctgg
     5221 ctttcatctc cctttaatgt ctcttccggt gatgcccggc agcccacact ggcggtgcac
     5281 agccaggcat ttcataccga acgagcctgt cgcgatgcat tgcgcgccat taaaaaagag
     5341 aatgttggcg aattttctct gcggggcgtt tgtgtttctg acggaaatac gaaggaggaa
     5401 gctgagagct catgacggat attactgaca gacatacact tcgccgtatg tcctggtcgg
     5461 aactcttcac ggcagcacag gaagcggaat ttcagcgtga ttacgagcgt gccagaatag
     5521 tgtggtcatt cgccttacat gttgcaacaa ccacgattaa caaaaatctg gccatagctc
     5581 atattcgacg ctgcgatact ttgttgcata aatcaaaaac agtacccgga aacaacacgg
     5641 ggggccggtc agtgtgcctg agacctcaac atccccggag atgactgaca ttccggactg
     5701 acgcagttgc tgcatatctg tggcaatacg ttgtatccag ccagacaggc tatcccgcgt
     5761 agtgacaatt aacccgcttt cctgcaccag cttcagccgg gccgtcgggg attttaacag
     5821 ccggaaatat cagtaaaaca acatttcatt atgccatggt gtacgcggcc agtgtacgct
     5881 tacttaagga atgagggata aattggatat tattacttca ctttgctttt ttatgagatt
     5941 catcacataa gtggggtttt actgttaaat aatctgaatt tatttgatat ccgtaaagca
     6001 gatgtctcag ttcactgttt ccaacttttg ttattcctct ggggcgaatg ctgtttccgc
     6061 tggaatattc acctggcacc agtcccagaa aagccatgag ctgttcagga ttggaaaatc
     6121 gcgaaaaatc acccagttca gcaaccaggg ttattgcgat tagctgacca acacctttaa
     6181 gagcctgcaa ctggcagacc agattgcaca gagatcactc aggtaacagg cgagatattt
     6241 cctgatcgag ttgaagtatg cgatcttcaa tctgctccag attctgacaa taatgttgaa
     6301 agcaatctgt tgagatagca gaccaaatga ttgattaact aaccaggttc gatgtttgcc
     6361 ggttcagtgc ttcctggcat aacgtttgtc tgttcgtgtc acgttttgca gcggcacggg
     6421 ctctgattaa atcccgcata gcttcatgag tcagctcagg gagccagact gggggaacct
     6481 caccatccct cagtaacctg gcgagagaaa ttacgtcccg atggtcattt ttgatcctgt
     6541 ctgccggtga tttgggtata cgggaagggg gcaataacca gacattttat attcatcact
     6601 gtgagttgat gatatagccc aaacccacag ggaccacctt catagcagga aagaatgttt
     6661 ccgtaaggtc cctgcaattt ctgacataat cgatgtacag ccagggcttc gttgttaatc
     6721 gtaccataat aacgaatttc gccaagccat tcaggtgtag caatcgccaa ggtttcttta
     6781 tgaacaacca gcccgacgta agctgcaata ttttccgaat ccatgatgac ctctggaata
     6841 tcccaccaat gctatagtga gtatataaag gtcatatcat ctacgtgttc ttcggcaacg
     6901 atcacagtgg agagcgtgga gcgctactgt acgggctgat tggcacctgc cgactgaaca
     6961 gtatcgaccc agaagcgtat ttgcgccata tcctgggcgt actactggaa tggccttcca
     7021 accgtgttga cgaacgctcg ccatggaatg tagtatatta ccaataaata accgtcaata
     7081 cggcactcgg ttaacgctta caaataagaa tagacaggac aaatgtaaac tacatttgca
     7141 tattctgtat aacattatca cacaagagaa tgacaccaac atttctctgg tgtataattc
     7201 ttttttgtgc tcgtaaaatt aatgttgcgg agtaatatac ctcctttgtt atatttaaat
     7261 cattcaataa aacgcctaat tttgcagggc tattactgga taaataatta ataatcttct
     7321 gttccattta atatagaata acctgttcag ataataatac tttatatcca actatataac
     7381 atattttttg tcatgttttt tataaaactt taaattaatt gcaaaatggc atctgaatcg
     7441 atcatagttt ggcattcgaa tcgatcatga tttggctctc ttgctccggc tttatccact
     7501 tcctctcaga cgacagtcag tgtcctgctc tgactgaacc tctgcaccgt tgttacgtac
     7561 cagcgtttgt ttatctatta gagtcacttc agtaggcaca tcagatgcca acgcaacatt
     7621 ccctgccata agcgcagcca gaacgcctgt tacaactaaa ctattcctgg tgatattgct
     7681 cattctcttt ctccatttgt cgaggatttg ggcgttgatt ttcgacgagc tccagttgca
     7741 actccagtct taaggttata tcgggatagc acacaagtgt tacacacata aacaaaaaat
     7801 acccaaaaag tattattcca taacatttat tcatggaaga taacaatgaa acgattcagc
     7861 tatatttttt aaactaaaaa atactttata agaacatccc aattttggag gcgatactca
     7921 ttctccccct gataactaaa cactctcgtc cgacagggcg tggaaactat caataaccac
     7981 agtaatgatg ctgactttgt tttcgtttaa ggaaatgaca tgcacgatta cctgcttttt
     8041 ttactgggac ttacccccct tctggcgatg atcattttga ttctgaaagt caaaatgccc
     8101 atacactatt ctgtgcttag cactctcctg ctcacgctaa tccttggaga ggttttctgg
     8161 cagacgccag ttatggatat gggatctgct gtggtatacg gcgcaatcaa aggtttgtgg
     8221 ccgattgtga ttgtcatcct tgctgccatt tacagctata acctgatgaa tgcgacaaaa
     8281 agcatggata ttctgagaaa tgttctggcg agcgttagtg acgataaacg catccaggtt
     8341 cttcttatct catggtgttt tggcggattt cttgaggcgg ccgccggata cggtacagcg
     8401 gtagcaatcc ccataggtat actcattgca ctgggcttta acccactaaa agcagcgatt
     8461 gcatcactgg ttgctaatac agtccctaca gcgtttgggg cagtcggtat acctgtctcc
     8521 gtgcttgctg aacaagttaa tttacctgta accacactca gcgggacgat tattcttcag
     8581 ctggcactgt tcaacattct gctccccttc gttatcgtca cgattatcgg tggaggtatg
     8641 aaagctatac gaggcgtagg gttaattacc ctgatgtgcg gtattagcac attgatacct
     8701 cagtatctgg tggctgttaa tcttggcgca gagctcccgg ctttcgcagg aagtcttgtc
     8761 agtctgatcg ttatagtctt tatggcaaaa aaccgtaaag aaaaaacaga acctgaattt
     8821 cttattgaga gttcagcaga tagcgccagc gcactttcat actctcgtgg ggcgttgtta
     8881 cgtgcgtgct caatctacat tctgatcttt ctgttcattc tgctttgctc tccactgttc
     8941 ccgacgataa aaagtgcagt gtctcagatt gcctcgtcac tcaccttctc tctgagtaac
     9001 ggcagcaatc tgtcgatgaa aattgaatgg atcgcaaccc ccggtgttct gattatcatt
     9061 gccagtgtta ttggcggctt gatacagaaa gcatccctgt catgcctgtt aaacgtattt
     9121 gtcagcacgg taaaacaact taaaaactcc attattgcca tttctgcgat tgttgccatg
     9181 gcaacggtaa tggatgtctg tgggttgatt ggcactctgg cacagagcct tgtggatctg
     9241 actggtgggg cctacctctt cattgctccg gttatcggcg ctcttggtac atttgttact
     9301 ggtagtgaca caaactccaa cattctgttt ggtaaattgc agaccactgc ggctgaaaaa
     9361 ttgcatgtgg acccgctctg gctggctgcc gcaaatacat ctggcgcaac aggcggcaaa
     9421 atgatttctc ctcagagtat tgctatcgct gtttcagcca cccgtatgga tggtcagggc
     9481 agcgtaatca tgtcaggaac actgaagtac tgctttgctt atattgttat tcttggactg
     9541 aaagtaggcc ttgtctacca cctgttcatg gcctgagaaa aaacaacaaa aggacgtgga
     9601 atattaatca acaggagaaa tttctgtgaa tgttaacttt ttcgtcacct gcattggtga
     9661 tgcgtttaaa tcccggatgg cacaaaagag tgtgctgtta ctggagcaat tgggttgcaa
     9721 tgtccatttc cccgaaaagc agggatgttg tggtcagcca gcgataaatg gcggttatgt
     9781 tcgtgatgcc atcccgggca tgaaaaatct catatcttcg ctggaagaga atgactggcc
     9841 ggtagtatct cctgccgggt catgcactta cacgataaaa aattacgctc gctatctcag
     9901 tgaagaacct gagtgggcgg cacgagctga aaaagtggct gaacgcctgc atgatttgac
     9961 atcatttata gtaaatcatc ttggcatcac agatgttggg gcaaaactca atggtcgcgc
    10021 tgtgtaccat ccctcctgca gcctttcccg taagctgggt gtaaccagag agccgctttt
    10081 gctacttgag aatgtacagg ggcttgagct actgccgttt cacgctcagg aaacgtgctg
    10141 cgggtttggc ggcacattct ctgtaaaaat ggcggagatc tctggtgaaa tggtgaaaga
    10201 gaaagtcggg catattatgg atgttaaacc tgattttctt atcggggctg acgccagttg
    10261 cctgctaaac atcggcggtc ggcttaaccg tgaagggcat cctgtaaaag tgatgcatat
    10321 tgctgaagtc ctgatgcaca gatgaggtaa acagaatgtc tttgaaaaca agtgatatca
    10381 ggtttaaaga gcgtatccgt attcagattc aggacccgat catgcgaaaa gctgtggcta
    10441 acgcacagga gcgcataggg actaatcgac agaaaatggt tgatgaactg ggacactggg
    10501 aggaatggcg ttcacgagct gaacagattc gtcggcatgt tctggaaaat cttgatgctt
    10561 atttgtatca attgtccgaa aaggttactg cgaatggcgg gcatgtatat tttgcccgta
    10621 ccagagagga tgcaaccagt tacatactga aggtggcgaa agaaaaaaac gccaggaagg
    10681 ttgttaaatc caaatcaatg gtgactgagg aaattggcat gaatgccgtt cttcagaaag
    10741 ccgggatacc cgtgattgaa acggaccttg gggaatatat tcttcagcta gcacatgagc
    10801 caccatcgca tgtggttgtg ccggcaatac ataaagatcg ccatcaaatc cgcagagttt
    10861 tacatgaaca tctggggtat gacgggcctg aaactccaga agcaatgaca ttgttcatcc
    10921 gtgagaaaat cagacaagat tttctgagtg ctgaagttgg cgtaacgggg tgtaactttg
    10981 ccgttgcaga aacagggtca gtctgccttg ttacaaacga aggcaacgca cggatgtgca
    11041 ccacattacc taaaacacat attgctgtta tgggtatgga gcgtattgct ccgacattcc
    11101 aggaggtgga tgttctgatt accatgcttg caaggagtgc tgtcggggcg agactgacag
    11161 gttataacac ctggcttaca ggccccagag aagaaggaca tgttgatggt ccggaagagt
    11221 tccatctcgt tattgttgac aatgggcgtt cagagattct cggctcagaa ttcagcgatg
    11281 tattgcgatg tatccgctgc ggtgcctgca ttaatacctg cccggcttat cgtcagattg
    11341 gtgggcatgg ttatggttca atctacccgg ggccaatcgg cgcggttatt tccccattgc
    11401 ttggtggata tgaagacttt aaggatttac cctatgcctg ctcattgtgc actgcctgca
    11461 atggggtatg tcctgtaaaa atacctctgg caacgctaat acaaaaacat cgccaggtta
    11521 tggcagagtc tgaaatcaca ccattaacgg aacgccgtat aaccagagca ttcaattaca
    11581 taaacagcca tccggcgttg tggaaggtag gtatggttgc tggggccaaa acagcccgtc
    11641 tgttcatcag gaaaggaaaa acgccgatta gtgttggagc gataaaagaa tggactgaag
    11701 ccagagacct gccatcacct gatggagaaa gcttccgtag ttggttcaag cgacataaga
    11761 aggaggggta atttatggaa aacagagagg aatttctcgt tgcgatcgcc agaaagcttg
    11821 gcagaccagt aagacatatt cccgaagcaa tgccggagcc tgtaaatacg ctggctacga
    11881 cacgtttaac agagttgaca tcagaccaac gctgtgaggc gtttattaaa tttgcctcgg
    11941 aagtgatgct tgccgagtgt gttctggtgt cgcctgagaa cgcaccatca acagccttag
    12001 acatctgttg gaagttcggt tccgggccag tcattatcag caacgatcaa cgactggtcg
    12061 atacagggat tacgccattg ctacaaaaag aaatggacgc tgctctgtgg gatccggaga
    12121 agggcgatga aaatatccat cttgctgaaa aggcaaaaac tggtgtcgtt ttcgctgagt
    12181 atgggctgac agaatcagga ggcattgtct tattctccgc accagaacgt gggcgctcag
    12241 taagcctttt gcctgaagca tcattatttg ttttacgtaa aagcaccatt ctccccagag
    12301 tggcgcagct tgcgcaaaaa cttcatgata tggctcagga aggacaacgg atgccgtcct
    12361 gtataaatct gattggaggc ccaagttcta ctgcagatat agaactgatc aaagttgttg
    12421 gcgtacatgg tcctgtacat gcaacctatt tgatcattga tgattgcgat taacgattgc
    12481 taataaaaaa atggccttaa ggtgatattt cgtcttgtga aaaggcaaca atagactaaa
    12541 ggttaggcat aattgtgttt ctttatgcct ccgcagcaat ttgtggaggc ttttttgctt
    12601 actccaaaca gtttggcttc ttgaaaaagt catatcccaa acctattgac gcttataaaa
    12661 aagtaaagaa tggtgagtaa gcgtaaactg accaccgtat gtagccatga tgccagtatt
    12721 ggtaacgtag gtgcacccct atctattttt gtagaaccgg agtaaacaga ccagatagtt
    12781 taaatgagct ggttatgcgt aacata
//
LOCUS       DAZFKF010000129        12732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFKF010000129 DAZFKF010000000
VERSION     DAZFKF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.131"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            54..1178
                     /locus_tag="ACHKA1_004502"
     CDS             54..1178
                     /locus_tag="ACHKA1_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO5682060.1"
                     /translation="MFQKIKIRKMTISRALDKYLKTVSIHKKGHLQEFYRVNVIKRHP
                     IAERYMDDITTVDIANYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGT
                     CRMNPVELVRKPKISSGRDRRLTSGEERRLSRYFKEKNQALYVIFHLALETAMRQGEI
                     LSLRWEHVDLQHGVAHLPTTKNGAPRDVPLSRKARSYLQMLPTQLNGNIFSYTSSGFK
                     SAWRTALHELKIENLHFHDLRHEAISRFFELGTLNVIEVAAISGHRSLNMLKRYTHLR
                     AYQLVSKLDARRKQTSKIAPYFVPYPATVENRNGQVVVTLSDFDLETSAATKEQAIFH
                     ASVLLLRTLAQAAQRGERVPTPGELPTNIDERVMICPLTN"
     gene            1190..1843
                     /locus_tag="ACHKA1_004503"
     CDS             1190..1843
                     /locus_tag="ACHKA1_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682061.1"
                     /translation="MIEQLQSDFLFWIFIYFLIVGLLIRNIHLTRKIKSIEKNKMFPA
                     EEYAVLDIEQHMSNKYIGRLVTRFMKRTNGAPLLIVFPEYDKGDNNEDKNSPPFQLVK
                     LTSDINNIKEIYSNDDLTCFLNNISAQKNLYTTHSGPSQAWIEHAYPLQQVTILLQGT
                     RHSEREDIISQLEIILSRLKNGENTGFEHDDDFGYSFQYDAAAIGKSSFFDEAAGRK"
     gene            1873..2408
                     /locus_tag="ACHKA1_004504"
                     /pseudo
     CDS             1873..2408
                     /locus_tag="ACHKA1_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542050.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            2424..2828
                     /locus_tag="ACHKA1_004505"
     CDS             2424..2828
                     /locus_tag="ACHKA1_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO5682062.1"
                     /translation="MALSPKEKKELQQRFEKQLLPDLDMSFELNGEVYKFNFDKNESG
                     DYIDSFTQAVWGGYQDAEEWEKYGKHQETELPECPEPVEGWCDCDKCKSMIFHKEKNI
                     YVCLSCHKQTEPFLPIDWNSNDYDWLPYEDEY"
     gene            3155..3484
                     /locus_tag="ACHKA1_004506"
     CDS             3155..3484
                     /locus_tag="ACHKA1_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682063.1"
                     /translation="MVTIIIGVSRVIDYKTAEMSVDIPPFPEWNSGHYIEIPGEGIVF
                     TGEKSDDGSRCYSGVIESHYSTDDWAFILSEYKNWLKDKERKFSYLFVLRNGELVHHE
                     VNKSELL"
     gene            3510..4097
                     /locus_tag="ACHKA1_004507"
     CDS             3510..4097
                     /locus_tag="ACHKA1_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6012 family protein"
                     /protein_id="HFO5682064.1"
                     /translation="MYLHIVPKLFHLMANKCTLKSISIPELDLIIDGESLSVGRPWPN
                     KCVWVGMRKSRKSVNGLILQTDKKLRWFTTRYTWDIENMGLIHHQVNTYIEDNEFDMV
                     SQEILLNGSFDKWSDRVHSAYENKPPARIQPKMESLLNKPGENSHDVWEEFEWGDFLL
                     SREESLLLYTIQSERLSTDCSLFKRQPSIESALVI"
     gene            4122..4697
                     /locus_tag="ACHKA1_004508"
     CDS             4122..4697
                     /locus_tag="ACHKA1_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO5682065.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESTHPF
                     HVCYGGYLLNPGVSDSDISRKIKDQTGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGH
                     QNIVPFAALIKADECIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCERR
                     FKQPVSEDNWQQAVSEERAIRCAKRLIQCKG"
     gene            4702..6858
                     /locus_tag="ACHKA1_004509"
     CDS             4702..6858
                     /locus_tag="ACHKA1_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFO5682066.1"
                     /translation="MKLFIAEKPAVANDIVKALGGNFTRHDGWFESDNTIVTNCFGHI
                     IESQPPENYNPEYKEWKIETLPLRLYPVKYQPVESAAKQVKTVIELIRRADVTEIIHA
                     GDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANPKNNRDFRGLYLKAL
                     ARSVADAIYGLSMTRAYTIPAKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFY
                     YTMTGHFQRGADVIRANWKPGEFAPLTDRKLLDKTWANGTATSLAGKPATVEAAATDD
                     KKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQF
                     SEAPQIIDALKSVFDQPMDIDTTRKSKAFNSAKVTAHTAIIPTVSVPDVNALSTDERN
                     VYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVENAGDDE
                     AEVENDDSSFDLLCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALVRVADFVTD
                     PVIKKLLKDKDRDKKDEHGGIGTPATRASILETLKKRNYITLEKGKLIPTDTGYALID
                     ALPDIAVNPDMTALWAEKQTLIENGEMTIEQFVDELYNDLIPMISNANSAEIKVSPSA
                     PSGQSQRLSSPCPSCGKQIVIRPKSYSCTGCEFKIWNEFSGKKITQAQAEKLIKSGKT
                     DLIKGFKKKSGGTYDAVLVLEDKKTGKLGFPARAKK"
     gene            6892..7314
                     /locus_tag="ACHKA1_004510"
     CDS             6892..7314
                     /locus_tag="ACHKA1_004510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682067.1"
                     /translation="MLQLIAKSGRGRAPKPIAEALAGGRSLDEFLIEKLFYSPPFLWR
                     ALSFEISVYFDDNSHVSFLGVGSTDFMVNDLLLFSVSVLVFLVIILMLHLTLKTDSIL
                     SECSEYRTKCDNLYHLCQMQEKELEHLRALIDAHNIDY"
     gene            7339..7659
                     /locus_tag="ACHKA1_004511"
     CDS             7339..7659
                     /locus_tag="ACHKA1_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682068.1"
                     /translation="MKFIILKKDKDSTIARNDSVFPVNNFFVVLTLVSSLANSFGIFF
                     PTISHSGNVIEMCFFVMFSTLLAAFSFVSTMGLANAVPPIKNVFPLLLLVLVLITNYN
                     IYFR"
     gene            7778..7984
                     /gene="hha"
                     /locus_tag="ACHKA1_004512"
     CDS             7778..7984
                     /gene="hha"
                     /locus_tag="ACHKA1_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO5682069.1"
                     /translation="MKTKQDWLFQLRRCSTLDTLEKIIEKNQNSLPPSELESFNAAAD
                     HRLAELTMNKLYDKIPPSVWKYVY"
     gene            8556..8996
                     /locus_tag="ACHKA1_004513"
     CDS             8556..8996
                     /locus_tag="ACHKA1_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682070.1"
                     /translation="MKEYEKAALIYSLKESNTLVLRIILISILVVGVISGAIYLYNLA
                     ISLFDSNPTSVQFTFCGVGCFIALLLQWISNVDANYRERIGDQPGTIKNIVQYLFAAI
                     ITISFITMALTAILVLKDSSNIWLNITFIALLLSIFVAIISVWG"
     gene            complement(9039..>12732)
                     /locus_tag="ACHKA1_004514"
     CDS             complement(9039..>12732)
                     /locus_tag="ACHKA1_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFO5682071.1"
                     /translation="YNLDSPKDYPRKDADYGKIISRVKGDLYKEHHSELKGELLSRIE
                     NEKIENYSVFKDIVAEYGELRIRNAGKTNEYLAVKLPGDKKYTNLKSPLFRQNYIETR
                     TLTLEKPTHKEIEKRLNTWLNKTSYEIKHIFNQAEKTRELYKTLSPSQQIDFLQERIK
                     EYDSREKLNERNSQQTSGRAGGYKSCPKKFARIRQSEATVGLSRMPQRGMVYGINGFT
                     RPDSVSVLSDISQRDLAEQLSQREHPGQDVRRDYDRQFTESGIKSLERSSFLCETMFQ
                     TLNEAAEKNEITTMAEIRRNIDPVRFLSSAAERFNIIPAQHKIRTAKDGSPRFSVGNR
                     NMNASDFLTKHINLAWKDAKSFLLEVYSQQLENTPYTRYPTYRRLTHHEARERLNSLN
                     LSEKTLRNTIRFERGKLYNDLREMRRELKLIPREQRDIAVGVIVYKKLTTLERLSELD
                     TEGRHIIRQYHADWHKDKDEMKALERLKSYLNFDEINAISADEPELSLQKAVDSQRRL
                     EEAKMVNSKLKDLVMDKQDSRIVYRDQESEKPVFTDKGNFVVAGKNPSKEEIGIMLEY
                     SREKFGGVLKLTGSEDFKKMCAEVAAEQDMKIILRPEQYQQMMLELKAELQGNKFEQV
                     ETQENSQESESRIEKGDALKEQATEQAQATEQAQATEQAQATEQAQATEQAQVAEQAT
                     FSYDPGVITRANTLDSQMLSKGTNGEFGYLKSLDSDENEIWEVLGHVPGDSDDIFSVA
                     SFDNENDAKEFCNIVNELGIDRTQALIQEQLTSQHDQATAQEHNKQEIYCVNFSHLHD
                     ENEGVVFHSKDAAIDFYEGNKSSAIEKYESNYLNGDGFETIVLMSKTVSTDELSSYPE
                     GGIVTLDKPFEVIANSYEEYHTPVYAVSFSKDEFSEDIKTFESLHDATEYKNNILQEH
                     GLNQDDILITPVTREEIAFKGIKDAVNDANMAVMEQAGDSSRESPEEILASISANEHM
                     ISGLENFLVKDRSQFSSCNGDIVVEAEITRGEGGLYHLAVAGKYGLERGDAVARVDVT
                     EQQFAAITGKTPSEVLTGDQTSARVPVITGIHFSTRAIENVNKLEQQKDYVYFSTHEG
                     LNAEIKDFSSLKDAIEWGRVECELHDLNKRDTVIYRVESEHISQGIDAVMKNAERVER
                     HEIEKAQGRDCTPEDGKILEAIDRFEDKFRGEGLKFEREKAESDLLNHGFTREMAEDA
                     LGKQFVQAREDHQESQQQRDDSQDMH"
BASE COUNT         3681 a         2689 c         2556 g         3806 t
ORIGIN      
        1 tgcatttcga agcaagaatc tctaccttcc cccctttttt gtctggaggg gatatgtttc
       61 agaaaatcaa aattcgcaaa atgacaatca gccgggcgct ggacaaatac ctgaagacag
      121 tatccatcca caaaaaaggt catctacagg agttttatcg cgtaaatgtg attaaacgtc
      181 accctattgc tgaacgctat atggatgaca ttaccactgt tgatattgcc aattatcggg
      241 atcaacggtt ggcgcaaatt aacccccgaa cggggcgtca aataaccgga aacaccgtcc
      301 gtcttgaact tgccctgttg tcatcattat tcaatatcgc ccgtgtggaa tggggaacat
      361 gccgcatgaa tcctgtcgaa ctggtcagaa aacccaaaat cagtagcggg cgcgatcgtc
      421 gcctgacttc aggagaagaa cgccgacttt cgcggtattt caaagaaaaa aaccaggctc
      481 tttacgtcat ttttcatctg gcactggaaa cagcaatgcg acaaggtgaa atcctgtcat
      541 tgcgttggga acacgtcgat ctgcaacatg gagtagctca tcttccaaca accaaaaacg
      601 gtgctccacg cgatgttccg ctatccagga aagcccgtag ttacctgcaa atgctgccga
      661 cacagcttaa cggtaatatt ttttcttata cctcttcggg attcaaaagt gcctggcgaa
      721 cagcacttca cgaactgaag atagaaaatt tgcattttca tgacctccgt catgaggcta
      781 tcagtcgatt tttcgaactt ggtacattga acgtcattga ggttgccgct atttcaggcc
      841 atcgctccct gaacatgttg aaacgataca cacaccttcg ggcttatcaa cttgtcagta
      901 aactcgatgc ccgacgcaag caaaccagca aaatcgctcc ctattttgtt ccctacccgg
      961 caacggtcga gaacagaaac gggcaagtcg ttgtgactct gagtgatttc gacctggaaa
     1021 caagcgcagc cacaaaagaa caggccatat ttcatgccag cgtgttgctg ctacgaacac
     1081 tggcacaagc cgcccagcga ggagaacgcg tccctactcc tggcgaactt cctacaaaca
     1141 ttgatgaacg ggtaatgatt tgtccattaa caaattagag gattcactta tgatagaaca
     1201 actacagagt gattttttat tctggatatt tatttacttc ctgatagtag gtttgcttat
     1261 cagaaatatt catcttacca gaaaaattaa atccatcgaa aaaaacaaaa tgttcccagc
     1321 ggaggaatat gctgtcctgg acattgaaca gcatatgtcc aataaatata ttgggcggct
     1381 agtaacccgt tttatgaaga gaactaatgg tgcacctttg ttaattgtgt ttccagaata
     1441 cgataaagga gacaacaatg aagacaaaaa ttctccacca ttccaacttg taaaactaac
     1501 atccgacatt aacaatatta aagaaattta ttccaatgat gatttaacct gttttctgaa
     1561 taatatttcg gcacaaaaaa atttatatac aacgcactct ggacctagcc aggcatggat
     1621 tgaacatgca tatcctttgc aacaagtaac tattctctta caaggcactc gtcatagtga
     1681 aagggaagat ataatcagtc aacttgagat tattctttcc agactaaaaa atggagaaaa
     1741 tacaggtttt gagcatgacg atgattttgg atattcattt cagtatgatg ccgcagcaat
     1801 aggaaaatca tcatttttcg atgaagcggc aggaagaaaa taagaataac gctctatcca
     1861 ttgcacagac aaattaacgt taatctttta tgaaaatctt attaataata tttatttatc
     1921 tgcttaccgc ttttcagtct gcaaatgcag gtggaatacc ttatacattt aaccagaaaa
     1981 ttcttcaggg gaaagttatt cgcgttctcg atggcgacac catagaaatt aaaacactac
     2041 ccgcaaaaat tgttgtgtat gaagttccga tccgagtccg attgataaac atcgatgccc
     2101 ctgaaaagaa acaacctttt ggacgctggt caactagtca gcttaaaact ctggttgcag
     2161 gtaaacaggt gacagtctct tattcacata aagaccgcta cggacgcatt atcggtcatg
     2221 tgttcacgac gaacggcacg gatgccagcc gctttatggt tcagtctggc gctgcatggg
     2281 tatatgaacg ttacaacgtg gatgagtcat taccggctct gcaacgggaa gcgcaggaac
     2341 aaaaacgcgg tttgtgggcc gatgcaaatc cggttccacc gtgggagtgg cgatacaaac
     2401 ataactaact gatagaggtt tttatggctt tatcacccaa agaaaagaaa gagttacaac
     2461 aacgcttcga aaagcaatta ctacctgacc ttgatatgtc atttgaacta aatggagaag
     2521 tatataaatt taattttgat aaaaatgagt caggtgacta tatcgatagt tttacacaag
     2581 cagtatgggg aggttatcag gatgccgagg aatgggaaaa atatggtaaa catcaggaga
     2641 cagaattacc tgaatgccct gaaccagtcg agggttggtg cgactgtgat aaatgtaagt
     2701 cgatgatttt tcataaagag aaaaatattt atgtttgcct ctcctgccat aaacaaacag
     2761 agccatttct tccaattgac tggaactcaa acgattacga ttggctacct tatgaagatg
     2821 aatactaatg ctgaatactt caaaatcata ataactgaaa tggcgacatg aaaattaacg
     2881 gggcaaaaaa tgcattcttc ttttggtttg ccatatccgg ctggtcactg gatgtattcg
     2941 ttatacgatt tactcgataa ttcagtgttc gtggtttgtt tctttgcctt ctgggtggca
     3001 acgggtcaat ttttattaag aactgtagac agaaaattta atatatctga aacagttgag
     3061 atggttatca tcgctttatt agggatacta atgactctgt ctttttatct ttgtgcaata
     3121 ttaaagactt acctttaaat cagagggtaa aaatatggta acgataataa taggtgtgtc
     3181 gagagttatt gactataaaa cggcagaaat gtcggttgat atccccccat tccctgaatg
     3241 gaacagtggt cattatatcg aaattccagg agaaggtatt gtattcacag gtgaaaagag
     3301 tgatgacggt agccgttgtt attctggtgt catcgaatct cattacagca ctgatgactg
     3361 ggcgtttatt ctttctgaat acaaaaactg gctgaaagat aaggaaagaa agttttctta
     3421 tctgtttgtc ttacgcaatg gtgaactggt tcaccatgag gtcaataaat cagagcttct
     3481 ttaactacgc taaaaaaagg ataatcgata tgtacctgca tattgtccct aaactttttc
     3541 atcttatggc gaataaatgc actcttaaat ccatctccat acctgaactt gatttaataa
     3601 ttgatggcga atcattgtct gtggggaggc cgtggcctaa taaatgtgta tgggttggaa
     3661 tgagaaaaag cagaaaatca gttaatggtc ttattttaca aacagataaa aaattaagat
     3721 ggtttactac cagatacacc tgggatattg aaaatatggg gttgattcat catcaggtta
     3781 atacctatat agaagataat gagtttgata tggtgtcaca ggaaattctt ttaaatggtt
     3841 cttttgataa gtggagtgac agggttcatt cagcttatga aaataagcct ccagccagaa
     3901 tccagcccaa aatggagtcc ttattaaata agcctggtga aaacagtcat gatgtgtggg
     3961 aagagttcga atggggtgac ttccttctgt ccagagaaga aagcctttta ctttacacga
     4021 ttcagtctga acgattgagt acagattgct ctttattcaa aagacaaccg tctattgaaa
     4081 gtgcattagt tatataaatc agatactttt tgagggttat tatgcaatat gcattgtttg
     4141 atggatttga acgaaagttt ttgctggatg ctcttgaatt tggtgttctg aaggactgga
     4201 aagaaaatcc ggtaaaagaa cttcctgata ttgatgaatc tactcacccc ttccatgtct
     4261 gttatggtgg atatttatta aatcctggtg tttcagattc agatattagc agaaaaataa
     4321 aagatcagac aggattctgg ttggcagcta ttgatgatac ccgcatggat tgtcattcaa
     4381 tagcttatta tgatattcac acactccctc tgatttcgtg tggtcatcaa aatatagttc
     4441 cttttgccgc gttaataaaa gctgatgaat gcatcatttc aaaaattgct tcgtattctg
     4501 gttttgctgt aacagccttt ttgagaatta aagagtggga tattgcaact aatatactta
     4561 accgtgaggg aatttttgcc tttaatggct gtgagcgcag attcaaacaa cctgtaagtg
     4621 aagataactg gcaacaggca gtatcagaag aacgcgctat ccgttgtgcc aaaagattaa
     4681 ttcaatgtaa aggataacaa aatgaaactt tttatcgcag aaaaacccgc agtagcaaat
     4741 gatattgtta aggcgcttgg gggcaatttt acccgtcatg atggctggtt tgaaagtgat
     4801 aatactattg tgactaactg ttttggtcat attatcgaat cacagccccc cgaaaactat
     4861 aatcctgaat acaaagagtg gaagattgaa acactccctt tgcgtcttta tcccgtaaaa
     4921 taccagcctg tagaaagcgc cgcaaaacaa gttaaaacgg ttattgagct tatcaggcgt
     4981 gctgatgtga ccgaaattat tcatgctggc gatcctgatg acgaaggaca gctacttgtc
     5041 gatgaggtcc tggaatatgc aggcaacaca aaacccgtta agcgcgttct gatcaacgac
     5101 aacacccttc cggcagtgaa aaaggcgctg gcaaatccca aaaataaccg tgacttcaga
     5161 ggactctatc tcaaggctct ggcgcgttct gtagccgacg cgatatacgg tttatccatg
     5221 acgcgtgctt acacaatccc ggcaaaaact aaaggctata aaggtgtttt gtctgtcgga
     5281 cgggtccaga cacccgtgct tggcctgatt gtaaatcgca cccgtgccaa taaaaatcat
     5341 aaatccagct tctactacac catgaccgga cattttcaac gcggcgctga tgttatcagg
     5401 gcgaactgga aacctggtga gttcgcaccg ctgacagacc gcaaattgct tgataaaacc
     5461 tgggcgaatg gaacagcaac atcacttgca ggaaaaccag ccacagttga agcggcagca
     5521 acagatgata aaaaaacggc tgcaccgttg ccctttaacc tggtacgact tcagcagtac
     5581 atgaacaaaa aattcaaaat gactgctcaa aaaacgctgg atatcacaca gcagctacgc
     5641 gaaaaataca aagcaattac ttacaaccgt tcagattgtt catatctttc agacgaacaa
     5701 ttcagtgaag caccacaaat aatcgatgcc ctgaagtcgg tgtttgatca gccaatggat
     5761 attgatacga cacgtaaaag caaggcattt aacagcgcaa aagtaacagc gcatacagcc
     5821 atcatcccaa ccgttagcgt accggacgta aacgcactga gcaccgacga acgcaatgtt
     5881 tacctggcaa ttgcacaaca ctatcttgtc cagttcatgc ctgaaaaggc atatcaggaa
     5941 gtatccgtcg ccattcagtg tggtgatgag tcgttttatg cccgtgccag gaaaactaca
     6001 gacagcggat ttgaagcgtt tcttggtgtg gaaaatgccg gggacgatga agcagaagtc
     6061 gaaaatgatg attcctcttt tgatcttctc tgtaaaattc gcacaggaga aacagtgacg
     6121 acaaaagaag tggttgtgaa tgagaagaaa acaacgccac cgccattatt caccgaagcc
     6181 actttacttg ccgcgcttgt tcgtgtcgca gattttgtca ctgatccagt gattaaaaag
     6241 ttactgaagg ataaagatcg ggataaaaaa gacgaacatg gcggtattgg tacgccagct
     6301 acgcgggctt ccatattgga aacactaaaa aaacggaact atatcaccct ggaaaaagga
     6361 aaacttattc cgacagatac cgggtatgcg cttattgatg ctctgccgga tatagctgtt
     6421 aatccagata tgacggcatt atgggcggaa aaacagacac tcattgaaaa cggagaaatg
     6481 accatcgaac agttcgttga cgaactgtac aatgatctga taccgatgat cagtaatgcg
     6541 aacagtgctg aaataaaagt ttcccccagc gcgccgtctg gtcagtcaca acgcctgtcc
     6601 tctccttgtc cttcctgtgg taaacagatt gttatcaggc caaaaagcta ttcctgtaca
     6661 ggatgtgaat ttaaaatctg gaatgagttt tctggcaaga aaatcaccca ggcacaggcc
     6721 gaaaaactta ttaaatcagg aaaaaccgat ttgattaagg gatttaaaaa gaaaagcggt
     6781 ggaacgtatg atgcagttct tgtccttgag gataagaaaa cagggaagct gggttttccg
     6841 gcaagggcta agaagtgaaa gcaaagcaga aatggctttt caggtcagga attgcttcag
     6901 ttaatcgcca agtctggtcg tgggcgagcg ccaaaaccga ttgctgaagc actggcagga
     6961 ggtcgttccc ttgatgagtt tctcatcgag aaattattct acagccctcc ttttctctgg
     7021 agggcgttat cctttgaaat atccgtttat tttgatgata attctcatgt ttcgtttctc
     7081 ggcgtgggga gtacagattt tatggttaat gatttgttgc ttttctctgt tagtgtatta
     7141 gtttttttag tgataatcct gatgttacat ttgaccctaa aaacagacag tattctttct
     7201 gaatgctctg aatatcgcac gaaatgtgat aatctctatc atctatgcca gatgcaagag
     7261 aaagagttag aacatctacg tgcactcatt gatgcgcata acatagatta ttagacgttg
     7321 ctttaggggg atggataaat gaaatttatt attttaaaaa aggataaaga tagcacaatt
     7381 gccagaaacg actctgtttt ccctgttaac aatttctttg tcgttttaac tcttgtgtct
     7441 tccctggcta actctttcgg aatatttttt cccacgatat ctcattctgg caatgttatt
     7501 gagatgtgtt ttttcgttat gttttcaaca ttactggctg ctttcagttt tgtaagtaca
     7561 atggggctgg cgaacgcagt cccccccatt aaaaatgtgt ttccgctttt gttgttggtt
     7621 cttgtcttaa ttactaatta taatatttac ttccgataag aacgggggcg cggcagcggc
     7681 ttttcttctg cccgctctcc cgtcaataaa cccagcagta gaaatcaaac atcgttgtag
     7741 ctattatagg gcaaggatgt tcttcaggaa ttgtacaatg aaaacaaaac aagactggct
     7801 ctttcagtta agacgttgct caacactcga cacacttgaa aagattattg aaaaaaacca
     7861 aaacagcctc cccccttctg agcttgagtc gtttaatgcc gccgccgatc acaggcttgc
     7921 agaactcaca atgaataaac tctacgataa aatcccccca tccgtctgga aatatgtata
     7981 ttagttctca tctattaata ttggattttt gatcgacaca aacatcagca atccctatac
     8041 acctttcata gaatgaattg aaccctgtat atagcctgtc tgaaatggat atctggcagg
     8101 ctatcttaat agtgttatag gtgatgtgaa tgtttttcca catgatcact ctttttctgt
     8161 tcttatcact attgatactc ctgataagga tattctccgc cagactaata attccggctg
     8221 tgggcgtgtt tctgccagta tcatcactct ggatgggatt tcatatgctc acacaaaatg
     8281 aatcctgtct ggataacatc gcctgtgttg ctcccccatt tggtcagttc agtaccctta
     8341 taggatggtt tttgattgcg gaatcaatat taaccgcatg tgctggcgta tattttctcc
     8401 gtaaaaataa aaaacgctgg tcacgtcatg atacagtcat gtttcttttt gcaagtggcc
     8461 tggcaatttt cagtacgtgg atgctggaac accaatgggt ttgaacttcc cttgacagtt
     8521 ttgttggttc ttatacttat agactggatt aaatcatgaa agaatacgaa aaagcggctt
     8581 taatatattc cttaaaggaa agcaatacat tagttcttcg gattatactc atctctattt
     8641 tagttgtcgg ggtgatttca ggcgcgatat acttatataa cttagcgatc tcattgtttg
     8701 attcaaaccc gacgtctgtg caattcacct tttgcggagt aggctgtttt attgctctat
     8761 tacttcaatg gatatctaat gttgatgcaa attacagaga aagaattggg gatcaaccag
     8821 gtacaataaa aaacattgta caatatttat ttgcagccat tattaccatt agtttcatta
     8881 caatggcatt aacagccata ttagtcttaa aagactcgtc caacatatgg ctcaacatta
     8941 cttttattgc attattactg tccatttttg tagctattat ttctgtttgg ggttgacttt
     9001 ttttcttaaa aagcccgaaa accgggcttt tttcttaatt agtgcatgtc ctgtgaatcg
     9061 tcacgctgct gctgcgattc ctgatgatct tctctggcct ggacaaattg ttttccaaga
     9121 gcatcttctg ccatttcacg agtgaatccg tgattaagca aatcggattc ggccttctcc
     9181 ctttcgaact tcagtccttc ccctctgaac ttgtcctcaa agcgatcaat tgcctcaaga
     9241 atcttgccgt cttccggcgt gcaatcccgt ccctgtgctt tctcaatttc atgacgctcc
     9301 acgcgttcag catttttcat tacagcgtca atcccctgag aaatatgttc agactcgacg
     9361 cgataaatga cagtatcgcg cttattgaga tcatgtagct cgcattcaac ccggccccac
     9421 tcaatagcat ccttgagaga gctgaaatct tttatttcag cattcagacc ttcatgtgtt
     9481 gagaaataga cataatcctt ctgttgttcc agtttattca cattttcaat agcccttgta
     9541 ctgaaatgga tacctgtaat taccggaaca cgagccgatg tttggtcccc cgttaaaact
     9601 tcagaaggtg tttttccagt gatggcggcg aattgctgct ctgtaacatc cactctggca
     9661 actgcatcac cacgctctaa gccatactta ccagcaacag caaggtgata aagaccacct
     9721 tcccccctgg taatttccgc ctccacaacg atatccccat tgcaggaact gaactgagaa
     9781 cgatctttaa ccaggaagtt ttcaagcccg gatatcatat gctcgttagc tgaaatactg
     9841 gcgagtatct cttctggtga ttctctggac gaatcccccg cctgttccat cactgccata
     9901 ttagcgtcat taacagcgtc cttaattcct ttaaaggcga tttcttctct tgttacaggt
     9961 gtgattaaga tatcatcctg gttaagcccg tgctcctgaa gtatattatt tttgtattca
    10021 gttgcgtcgt gcaatgactc aaaagtctta atatcttcac tgaattcatc cttcgagaaa
    10081 gacaccgcat acactggagt atggtattct tcatatgaat tggcaattac ttcaaatggc
    10141 ttatcaagcg ttactatccc cccctctgga taagaggata attcatcggt tgagacagtt
    10201 ttagacataa gcacaatagt ttcaaaacca tcaccattta aataattgct ttcatatttt
    10261 tctattgcag aagatttgtt tccctcataa aagtcaatgg cagcatcttt agaatgaaat
    10321 acaacgccct cattctcatc atggaggtga ctaaagttta cacagtaaat ttcctgttta
    10381 ttatgctcct gcgcggtagc ctgatcgtgt tgagaagtaa gctgctcctg aatcaaagcc
    10441 tgtgttctgt caattcctaa ttcattaaca atattgcaaa attcttttgc atcgttttca
    10501 ttatcaaaac tggcaacact aaagatatca tcgctgtctc caggtacatg ccccagcact
    10561 tcccaaatct cattttcatc gctatccaga gatttcaaat agccaaattc accatttgtg
    10621 cctttactaa gcatctgtga atccagggta tttgccctgg taataacacc aggatcatat
    10681 gaaaatgtgg cctgctctgc gacctgtgct tgctctgtgg cctgcgcctg ctctgtagcc
    10741 tgagcctgtt ctgtggcctg tgcctgttct gtagcctgcg cctgttcggt ggcctgttcc
    10801 ttcaatgcat cacctttctc tattctggat tcggactcct ggctattttc ctgtgtttcc
    10861 acctgctcaa acttattacc ctgaagttct gcttttaatt ccagcatcat ctgttgatac
    10921 tgctccgggc gtaaaataat cttcatgtcc tgctctgcgg caacttcagc gcacattttt
    10981 ttgaaatctt cagagccggt aagtttgagt acaccaccaa acttttccct ggaatactca
    11041 agcattattc cgatctcttc ttttgaggga tttttacccg caacgacaaa gttccctttg
    11101 tcggtaaaga caggcttttc cgattcctga tcgcgataaa caatcctgga atcctgctta
    11161 tccatcacaa gatcttttag tttggagtta accattttcg cctcttctaa gcggcgctgg
    11221 gaatccaccg ctttctgaag cgaaagttcg ggttcgtcag cagaaatggc gttgatttca
    11281 tcgaagttga gatagctttt gagacgttca agggctttca tttcatcttt atccttatgc
    11341 cagtcagcat gatattgacg aattatgtgt cgcccctctg tgtcgagttc agaaagacgc
    11401 tcaagtgtgg ttagtttttt gtaaacaatc acacccacgg caatatcacg ctgttcacgt
    11461 ggtattaact tcaactcgcg tctcatctct cgcagatcat tatacagctt gccacgctca
    11521 aacctgatag tatttcgtaa tgttttttcc gataaattca gtgagttaag acgctcacgg
    11581 gcttcatgat gcgtaaggcg tctgtaggtt ggatagcgcg tatatggcgt gttttctaac
    11641 tgctgtgaat aaacttcaag cagaaaggat ttcgcatctt tccaggcaag attaatatgc
    11701 tttgtcagaa aatcagatgc gttcatatta cgattcccca cagaaaaccg gggggagccg
    11761 tcctttgctg ttctgatttt atgctgcgct ggaataatat taaaacgctc ggcggcagaa
    11821 gacagaaacc tgacaggatc aatatttctg cgaatttctg ccatcgttgt aatttcgttt
    11881 ttctctgccg cctcattcag agtctgaaac atcgtttcac acaaaaaaga agagcgttca
    11941 agacttttta ttcctgactc tgtgaattgt ctatcataat cccgtcgcac atcctgacca
    12001 ggatgttctc tttgcgataa ttgctctgcc agatcacgct gcgaaatatc tgacaataca
    12061 ctgacagagt cgggtcttgt gaatccattg attccgtaaa ccataccgcg ttggggcata
    12121 cgtgacaatc cgactgttgc ttcagattga cggattctgg caaacttttt aggacacgac
    12181 ttgtaacctc ccgctcgtcc tgacgtttgc tgagaatttc tttcgttaag tttttctctt
    12241 gagtcatatt cttttatcct ctcttgcagg aaatcaattt gttgtgaagg gcttaatgtc
    12301 ttgtaaagtt cccttgtttt ctccgcctgg ttaaaaatat gcttaatctc atagcttgtt
    12361 ttatttaacc aggtattaag tctcttttct atctctttgt gtgttggttt ctcaagtgtt
    12421 aatgtccttg tctcaatgta attctgacga aaaagaggac tttttaagtt tgtgtatttt
    12481 ttatctcccg gcaattttac agccagatac tcattggttt taccagcatt tcttatacga
    12541 agttcaccat actctgcaac aatatctttg aatacagagt aattttcaat tttctcgttc
    12601 tctatacgcg aaagcaactc acctttcaat tcagagtgat gctctttgta aagatctccc
    12661 ttaactctac ttataatttt tccgtaatcc gcatctttac gcggatagtc tttagggcta
    12721 tcaagattat at
//
LOCUS       DAZFKF010000130        12452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFKF010000130 DAZFKF010000000
VERSION     DAZFKF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.132"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(119..1402)
                     /gene="gltA"
                     /locus_tag="ACHKA1_004515"
     CDS             complement(119..1402)
                     /gene="gltA"
                     /locus_tag="ACHKA1_004515"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO5682072.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
     gene            complement(1608..1715)
                     /locus_tag="ACHKA1_004516"
     CDS             complement(1608..1715)
                     /locus_tag="ACHKA1_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682073.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2096..2500
                     /gene="sdhC"
                     /locus_tag="ACHKA1_004517"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2096..2500
                     /gene="sdhC"
                     /locus_tag="ACHKA1_004517"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO5682074.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2494..2841
                     /gene="sdhD"
                     /locus_tag="ACHKA1_004518"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2494..2841
                     /gene="sdhD"
                     /locus_tag="ACHKA1_004518"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO5682075.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2841..4607
                     /gene="sdhA"
                     /locus_tag="ACHKA1_004519"
     CDS             2841..4607
                     /gene="sdhA"
                     /locus_tag="ACHKA1_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO5682076.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4623..5339
                     /gene="sdhB"
                     /locus_tag="ACHKA1_004520"
     CDS             4623..5339
                     /gene="sdhB"
                     /locus_tag="ACHKA1_004520"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO5682077.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5640..8441
                     /gene="sucA"
                     /locus_tag="ACHKA1_004521"
     CDS             5640..8441
                     /gene="sucA"
                     /locus_tag="ACHKA1_004521"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO5682078.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8456..9673
                     /gene="odhB"
                     /locus_tag="ACHKA1_004522"
     CDS             8456..9673
                     /gene="odhB"
                     /locus_tag="ACHKA1_004522"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO5682079.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9949..11115
                     /gene="sucC"
                     /locus_tag="ACHKA1_004523"
     CDS             9949..11115
                     /gene="sucC"
                     /locus_tag="ACHKA1_004523"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO5682080.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11115..11984
                     /gene="sucD"
                     /locus_tag="ACHKA1_004524"
     CDS             11115..11984
                     /gene="sucD"
                     /locus_tag="ACHKA1_004524"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO5682081.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
BASE COUNT         3015 a         3166 c         3393 g         2878 t
ORIGIN      
        1 gtgtaattat gtatttgtgt ccgattacgt ttttttgtcg atagacataa ctaacgaaca
       61 aaaaaaagcc cgccagatat tcaggcgggc taaacgatat atggttcaaa tcagataatt
      121 aatgtttaac atctgattta aagtctcgtt tttcatagcc ggtgtacaac tggcgcggac
      181 gggcgatctt catgccgtca ctgtgcattt cgctccagtg agcgatccag ccaacggtac
      241 gggccatagc gaagatgacg gtgaacatgg aagacggaat acccatcgct ttcaggatga
      301 taccagagta gaaatcgacg ttcgggtaca gtttcttctc gataaagtac gggtcgttca
      361 gcgcgatgtt ttccagctcc atagccactt ccagcaggtc atctttggtg cccagctctt
      421 tcagaacttc atggcaggtt tcacgcatta cggtggcgcg cgggtcgtaa tttttgtaca
      481 cgcggtgacc gaagcccatc aggcggaaag aatcattttt atctttcgca cgacgaacaa
      541 attccggaat gtgtttaacg gagctaattt cttccagcat tttcagcgcc gcttcgttag
      601 caccaccgtg cgcaggtccc cacagtgaag caatacctgc tgcgatacag gcaaacgggt
      661 tcgcacccga agagccagcg gtacgcacgg tggaggtaga ggcgttctgt tcatggtcag
      721 cgtgcaggat cagaatacgg tccatagcac gttccagaat cggattaact tcatacggtt
      781 cgcacggcgt ggagaacatc atattcagga agttaccggc ataggagaga tcgttgcgcg
      841 gataaacaaa tggctgacca atggaatact tgtaacacat cgctgccatg gtcggcattt
      901 tcgacagcag gcggaacgcg gcaatttcac gatgacgagg attgttaaca tccagcgagt
      961 cgtgatagaa cgccgccagc gcgccggtaa taccacacat gactgccatt ggatgtgagt
     1021 cgcgacggaa agcgtggaac agacgggtaa tctgctcgtg gatcatggta tgacgggtca
     1081 ccgtagtttt aaattcgtca tactgttcct gagtcggttt ttcaccattc agcaggatgt
     1141 aacaaacttc caggtagtta gaatcggtcg ccagctgatc gatcgggaaa ccgcggtgca
     1201 gcaaaatacc ttcatcacca tcaataaaag taattttaga ttcgcaggat gcggttgaag
     1261 tgaagccagg gtcaaaggtg aacacacctt ttgaaccgag agtacggata tcaataacat
     1321 cttgacccag cgtgcctttc agcacatcca gttcaacagc tgtgtccccg ttgagggtga
     1381 gttttgcttt tgtatcagcc atttaaggtc tccttagcgc cttattgcgt aagactgccg
     1441 gaacttaaat ttgccttcgc acatcaacct ggctttaccc gtttgttatt tggctcgccg
     1501 ctctgtgaaa gaggggaaaa cctgggtaca gagctctggg cgcttgcagg taaaggatcc
     1561 attgatgacg aataaatggc gaatcaagta cttagcaatc cgaattatta aacttgtcta
     1621 ccactaataa ctgtcccgaa tgaattggtc aatacttcca cactgttaca taagttaatc
     1681 ttaggtgaaa taccgacttc ataactttta cgcattatat gcttttcctg gtaatgtttg
     1741 taacaacttt gttgaatgat tgtcaaatta gatgattaaa aattaaataa atgttgttat
     1801 cgtgacctgg atcactgttc aggataaaac ccgacaaact atatgtaggt taattgtaat
     1861 gattttgtga acggcctata ctgccgccag gtctccggaa caccctgcaa tcccgagcca
     1921 cccagcgttg taacgtgtcg ttttcgcatc tggaagcagt gttttgcatg acgcgcagtt
     1981 atagaaagga cgctgtctga cccgcaagca gaccggagga aggaaatccc gacgtctcca
     2041 ggtaacagaa agttaacctc tgtgcccgta gtccccaggg aataataaga acagcatgtg
     2101 ggcgttattc atgataagaa atgtgaaaaa acaaagacct gttaatctgg acctacagac
     2161 catccggttc cccatcacgg cgatagcgtc cattctccat cgcgtttccg gtgtgatcac
     2221 ctttgttgca gtgggcatcc tgctgtggct tctgggtacc agcctctctt cccctgaagg
     2281 tttcgagcaa gcttccgcga ttatgggcag cttcttcgtc aaatttatca tgtggggcat
     2341 ccttaccgct ctggcgtatc acgtcgtcgt aggtattcgc cacatgatga tggattttgg
     2401 ctatctggaa gaaacattcg aagcgggtaa acgctccgcc aaaatctcct ttgttattac
     2461 tgtcgtgctt tcacttctcg caggagtcct cgtatggtaa gcaacgcctc cgcattagga
     2521 cgcaatggcg tacatgattt catcctcgtt cgcgctaccg ctatcgtcct gacgctctac
     2581 atcatttata tggtcggttt tttcgctacc agtggcgagc tgacatatga agtctggatc
     2641 ggtttcttcg cctctgcgtt caccaaagtg ttcaccctgc tggcgctgtt ttctatcttg
     2701 atccatgcct ggatcggcat gtggcaggtg ttgaccgact acgttaaacc gctggctttg
     2761 cgcctgatgc tgcaactggt gattgtcgtt gcactggtgg tttacgtgat ttatggattc
     2821 gttgtggtgt ggggtgtgtg atgaaattgc cagtcagaga atttgatgca gttgtgattg
     2881 gtgccggcgg cgcaggtatg cgcgcggcgc tgcaaatttc ccagagcggc cagacctgtg
     2941 cgctgctctc taaagtcttc ccgacccgtt cccataccgt ttctgcgcaa ggcggcatta
     3001 ccgttgcgct gggtaatacc catgaagata actgggaatg gcatatgtac gacaccgtga
     3061 aagggtcgga ctatatcggt gaccaggacg cgattgaata tatgtgtaaa accgggccgg
     3121 aagcgattct ggaactcgaa cacatgggcc tgccgttctc gcgtctcgat gatggtcgta
     3181 tctatcaacg tccgtttggc ggtcagtcga aaaacttcgg cggcgagcag gcggcacgca
     3241 ctgcggcagc ggctgaccgt accggtcacg cactgttgca cacgctttat cagcagaacc
     3301 tgaaaaacca caccaccatt ttctccgagt ggtatgcgct ggatctggtg aaaaaccagg
     3361 atggcgcggt ggtgggttgt accgcactgt gcatcgaaac cggtgaagtg gtttatttta
     3421 aagcccgcgc taccgtgctg gcgactggcg gagcagggcg tatttatcag tccaccacta
     3481 acgcccacat taacactggc gacggtgtcg gcatggctat ccgcgcaggc gtaccggtgc
     3541 aggatatgga aatgtggcaa ttccacccga ccggcattgc cggtgcgggc gtactggtca
     3601 ccgaaggttg ccgtggtgaa ggcggttatc tgctgaacaa acatggcgaa cgctttatgg
     3661 agcgttatgc gccgaacgcc aaagacctgg cgggccgtga tgtggtggcg cgttccatca
     3721 tgattgaaat ccgtgaaggt cgcggctgtg atggtccgtg ggggccacac gcgaaactga
     3781 aactcgatca cttgggtaaa gaagttctcg aatcccgtct gccgggtatc ctggagcttt
     3841 cccgtacctt cgctcacgtt gatccggtga aagagccgat tccggttatc ccaacctgtc
     3901 actacatgat gggcggtatt ccgaccaaag ttaccggtca ggcgctgact gtgaatgaga
     3961 aaggcgaaga tgtggttgtt ccggggctgt tcgcggtagg tgaaattgcc tgcgtatcgg
     4021 tacacggtgc taaccgtctg ggcggcaact cgctgctgga cctggtggtc tttggtcgcg
     4081 cagcgggtct gcatctgcaa gagtctatcg ccgagcaggg cgcattgcgc gatgccagcg
     4141 agtctgatgt tgaagcatct ctggatcgtc tgaaccgctg gaacaataat cgtaacggtg
     4201 aagatccggt ggcgattcgt aaagcgctgc aagaatgtat gcagcataac ttctcggtct
     4261 tccgtgaagg tgatgcgatg gcgaaagggc ttgagcagtt gaaagttatc cgcgagcgtc
     4321 tgaaaaatgc ccgtctggat gacacttcca gcgagttcaa cacccagcgc gttgagtgtc
     4381 tggaactgga taacctgatg gaaacggcgt atgcaacggc tgtttctgcc aacttccgta
     4441 ccgaaagccg tggcgcgcat agccgcttcg acttcccgga tcgtgatgat gaaaactggc
     4501 tgtgccactc cctgtatctg ccagagtcgg aatccatgac gcgccgaagc gtcaacatgg
     4561 aaccgaaact gcgcccggca ttcccgccga agattcgtac ttactaatgc ggagacagga
     4621 aaatgagact cgagttttca atttatcgct ataacccgga tgttgatgat gctccgcgta
     4681 tgcaggatta caccctggaa gcggaagaag gtcgcgacat gatgctgctg gatgcgctta
     4741 tccagctgaa agagaaagat cccagcctgt cgttccgccg ctcctgccgt gaaggtgtgt
     4801 gcggttccga cggtctgaac atgaacggca agaatggtct ggcctgtatt accccgattt
     4861 cggcactcaa ccagccgggc aagaagattg tgattcgccc gctgccaggt ttaccggtga
     4921 tccgcgattt ggtggtagac atgggacaat tctatgcgca atatgagaaa attaagcctt
     4981 acctgttgaa taatggacaa aatccgccag ctcgcgagca tttacagatg ccagagcagc
     5041 gcgaaaaact cgacgggctg tatgaatgta ttctctgcgc atgttgttca acctcttgtc
     5101 cgtctttctg gtggaatccc gataagttta tcggcccggc aggcttgtta gcggcatatc
     5161 gtttcctgat cgatagccgt gataccgaga ctgacagccg cctcgacggt ttgagcgatg
     5221 cattcagtgt attccgctgt cacagcatca tgaactgcgt cagtgtatgt ccgaagggac
     5281 tgaacccgac gcgcgccatc ggtcatatca agtcgatgtt gttgcaacgt aatgcgtaaa
     5341 ccgtaggcct gataagacgc gcaagcgtcg catcaggcaa ccagtgccgg atgcggcgtg
     5401 aacgccttat ccggcctaca agtcattacc cgtaggcctg ataagcgcag cgcatcaggc
     5461 gtaacaaaga aatgcaggaa atctttaaaa actgcccctg acactaagac agtttttaaa
     5521 ggttccttcg cgagccacta cgtagacaag agctcgcaag tgaaccccgg cacgcacatc
     5581 actgtgcgtg gtagtatcca cggcgaagta agcataaaaa agatgcttaa gggatcacga
     5641 tgcagaacag cgctttgaaa gcctggttgg actcttctta cctctctggc gcaaaccaga
     5701 gctggataga acagctctat gaagacttct taaccgatcc tgactcggtt gacgctaact
     5761 ggcgttcgac gttccagcag ttacctggta cgggagtcaa accggatcaa ttccactctc
     5821 aaacgcgtga atatttccgc cgcctggcga aagacgcttc acgttactct tcaacgatct
     5881 ccgaccctga caccaatgtg aagcaggtta aagtcctgca gctcattaac gcataccgct
     5941 tccgtggtca ccagcatgcg aatctcgatc cgctgggact gtggcagcaa gataaagtgg
     6001 ccgatctgga tccgtctttc cacgatctga ccgaagcaga cttccaggag accttcaacg
     6061 tcggttcatt tgccagcggc aaagaaacca tgaaactcgg cgagctgctg gaagccctca
     6121 agcaaaccta ctgtggcccg attggtgccg agtatatgca catcaccagc accgaagaaa
     6181 aacgctggat ccaacagcgt atcgagtctg gtcgcgcgac tttcaatagc gaagagaaaa
     6241 aacgcttctt aagcgaactg accgccgctg aaggccttga acgttacctc ggtgcaaaat
     6301 tccctggtgc aaaacgcttc tcgctggaag gcggtgacgc gttaatcccg atgcttaaag
     6361 agatgatccg ccacgctggc aacagcggca cccgggaagt ggttctcggg atggcgcacc
     6421 gtggtcgtct gaacgtgctg gtgaacgtgc tgggtaaaaa accgcaagac ttgttcgacg
     6481 agtttgccgg taaacataaa gaacacctcg gcacgggtga cgtgaaatac cacatgggct
     6541 tctcgtctga cttccagacc gatggcggcc tggtgcacct ggcgctggcg tttaacccgt
     6601 ctcaccttga gattgtaagc ccggtagtta tcggctctgt tcgtgcccgt ctggacagac
     6661 ttgatgagcc gagcagcaac aaagtgctgc caatcaccat ccacggtgac gccgcagtga
     6721 ccgggcaggg cgtggttcag gaaactctga acatgtcgaa agcgcgtggt tatgaagttg
     6781 gcggtacggt acgtatcgtt atcaacaacc aggttggttt caccacctct aatccgctgg
     6841 atgcccgttc tacaccgtac tgtactgata tcggtaagat ggttcaggca ccgattttcc
     6901 acgttaacgc ggatgatccg gaagccgttg cctttgtgac ccgtctggcg ctcgatttcc
     6961 gtaacacctt taaacgtgat gtcttcatcg acctggtgtg ctaccgccgt cacggccaca
     7021 acgaagccga cgagccgagc gcaacccagc cgctgatgta tcagaaaatc aaaaaacatc
     7081 cgacaccgcg caaaatctac gctgacaagc tggagcagga aaaagtggcg acgctggaag
     7141 atgccaccga gatggttaac ctgtaccgcg atgcgctgga tgctggcgat tgcgtagtgg
     7201 cagagtggcg tccgatgaac atgcactctt tcacctggtc gccgtatctc aaccacgaat
     7261 gggacgaaga gtacccgaac aaagttgaga tgaagcgcct gcaggagctg gcgaaacgca
     7321 tcagcacggt gccggaagcg gttgaaatgc agtctcgcgt tgccaaaatt tatggcgatc
     7381 gccaggcgat ggctgccggt gagaaactgt tcgactgggg cggcgcggaa aacctcgctt
     7441 acgccacgct ggttgacgaa ggcattccgg ttcgcctgtc gggtgaagac tccggtcgcg
     7501 gtacattctt ccaccgccac gcggtgatcc acaaccagtc taacggttcc acttacacgc
     7561 cgctgcaaca catccataac ggccagggcg cgttccgtgt ctgggactcc gtactttctg
     7621 aagaagcagt gctggcgttt gaatacggtt atgccaccgc agaaccacgc actctgacta
     7681 tctgggaagc gcagttcggt gacttcgcca acggtgcgca ggtggttatc gaccagttca
     7741 tctcctctgg cgaacagaaa tggggccgga tgtgtgggct ggtgatgttg ctgccgcacg
     7801 gttacgaagg gcaggggccg gagcactcct ccgcgcgtct ggaacgttat ctgcaacttt
     7861 gtgctgagca aaacatgcag gtgtgcgtac cgtctacccc ggcacaggtt taccacatgc
     7921 tgcgtcgtca ggcgctgcgc gggatgcgtc gtccgctggt cgtgatgtcg ccgaaatccc
     7981 tgctgcgtca tccgctggcg gtttccagcc tcgaagaact ggcgaacggc accttcctgc
     8041 cagccatcgg tgaaatcgac gagcttgatc cgaagggcgt gaagcgcgta gtgatgtgtt
     8101 ctggtaaggt ttattacgac ctgctggaac aacgtcgtaa gaacaatcaa cacgatgtcg
     8161 ccattgtgcg tatcgagcaa ctctacccgt tcccgcataa agcgatgcag gaagtgttgc
     8221 agcagtttgc tcacgtcaag gattttgtct ggtgccagga agagccgctc aaccagggcg
     8281 catggtactg cagccagcat catttccgtg aagtgattcc gtttggggct tctctgcgtt
     8341 atgcaggccg cccggcctcc gcctctccgg cggtagggta tatgtccgtt caccagaaac
     8401 agcaacaaga tctggttaat gacgcgctga acgtcgaata aataaaggat acacaatgag
     8461 tagcgtagat attctggtcc ctgacctgcc tgaatccgta gccgatgcca ccgtcgcaac
     8521 ctggcataaa aaacccggcg acgcagtcgt acgtgatgaa gtgctggtag aaatcgaaac
     8581 tgacaaagtg gtactggaag taccggcatc agcagacggc attctggatg cggttctgga
     8641 agatgaaggt acaacggtaa cgtctcgtca gatccttggt cgcctgcgtg aaggcaacag
     8701 cgccggtaaa gaaaccagcg ccaaatctga agagaaagcg tccactccgg cgcaacgcca
     8761 gcaggcgtct ctggaagagc aaaacaacga tgcgttaagc ccagcgatcc gtcgcctgct
     8821 ggctgaacac aacctcgacg ccagcgccat taaaggcacc ggtgtgggtg gtcgtctgac
     8881 tcgtgaagat gtggaaaaac atctggcgaa agccccggcg aaagagtctg ctccggcagc
     8941 ggctgctccg gcggcgcaac cggctctggc tgcacgtagc gaaaaacgtg tcccgatgac
     9001 tcgcctgcgt aagcgtgtgg cagagcgtct gctggaagcg aaaaactcca ccgccatgct
     9061 gaccacgttc aacgaagtca acatgaagcc gattatggat ctgcgtaagc agtacggtga
     9121 agcgtttgaa aaacgccacg gcatccgtct gggctttatg tccttctacg tgaaagcggt
     9181 ggttgaagcc ctgaaacgtt acccggaagt gaatgcttct atcgacggcg atgacgtggt
     9241 ttaccacaac tatttcgacg tcagcatggc ggtttctacg ccgcgcggcc tggtgacgcc
     9301 ggttctgcgt gatgtcgata ccctcggcat ggcagacatc gagaagaaaa tcaaagagct
     9361 ggcagttaaa ggccgtgacg gcaagctgac ggttgaagat ctgaccggtg gtaacttcac
     9421 catcaccaac ggtggtgtgt tcggttccct gatgtctacg ccgatcatca acccgccgca
     9481 gagcgcaatt ctgggtatgc acgctatcaa agatcgtccg atggcggtga atggtcaggt
     9541 tgagatcctg ccgatgatgt acctggcgct gtcctacgat caccgtctga tcgatggtcg
     9601 cgaatccgtg ggcttcctgg taacgatcaa agagttgctg gaagatccga cgcgtctgct
     9661 actggacgtg tagtagttta agtttcacct gcactgtaga ccggataaga cattatcgcc
     9721 ttctccggca attgaagcct gatgcgacgc ttaacgcgtc ttatcaggcc tacgggaagc
     9781 accgatgtag gtcggataag gcgctcgcgc cgcatccgac aagcgatgcc agatgcgacg
     9841 cttacacgtc ttatcaggcc tacgggtgac cgacaatacc cgggagcgat acgaaatatt
     9901 cggtctacgg tttaaaagat aacgattact gaaggatgga cagaacacat gaacttacat
     9961 gaatatcagg caaaacaact gtttgcccgc tatggcttac cagcaccggt gggttatgcc
    10021 tgtactactc cgcgcgaagc agaagaagcc gcttcaaaaa tcggtgccgg tccgtgggta
    10081 gtgaaatgtc aggttcacgc tggtggccgc ggtaaagcgg gcggtgtgaa agttgtaaac
    10141 agcaaagaag acatccgtgc ttttgcagaa aactggctcg gcaagcgtct ggtaacgtat
    10201 caaacagatg ctaatggcca accggttaac cagattctgg ttgaagcagc gaccgatatc
    10261 gctaaagagc tgtatctcgg tgccgttgta gaccgtagtt cccgtcgcgt ggtctttatg
    10321 gcctctaccg aaggcggcgt ggaaatcgaa aaagtggcgg aagaaactcc gcacctgatc
    10381 cacaaagttg cgcttgatcc gctgactggc ccgatgccgt atcagggacg cgagctggcg
    10441 ttcaaactgg gtctggaagg taaactggtt cagcagttca ccaaaatctt catgggcctg
    10501 gcgaccattt tcctggagcg cgacctggcg ttgatcgaaa tcaacccgct ggtcatcacc
    10561 aaacagggtg atctgatttg cctcgacggc aaactgggcg ctgatggcaa cgccctgttc
    10621 cgccagcctg atctgcgcga aatgcgtgac cagtcgcagg aagatccacg tgaagcacag
    10681 gctgcacagt gggaactgaa ctacgttgcg ctggacggta acatcggttg tatggttaac
    10741 ggcgcaggtc tggcgatggg tacgatggac atcgttaaac tgcacggcgg agaaccggct
    10801 aacttcctgg acgttggcgg cggcgcaacc aaagaacgtg taaccgaagc gttcaaaatc
    10861 atcctctctg acgacaaagt gaaagccgtt ctggttaaca tcttcggcgg tatcgttcgt
    10921 tgcgatctga tcgctgacgg tatcatcggc gcggtagcgg aagtgggtgt taacgtaccg
    10981 gtcgtggtac gtctggaagg taacaacgcc gaactcggcg cgaagaaact ggctgacagc
    11041 ggcctgaata ttattgcagc aaaaggtctg acggatgcag ctcagcaggt tgttgccgca
    11101 gtggagggga aataatgtcc attttaattg ataaaaacac caaggttatc tgccagggct
    11161 ttaccggtag ccaggggact ttccactctg aacaggccat tgcatacggc accaaaatgg
    11221 ttggtggcgt aactccaggt aaaggcggta ctacccacct cggcctgccg gtgttcaaca
    11281 ccgtgcgtga agctgttgct gctactggcg ctaccgcttc tgttatctac gtaccagcac
    11341 cgttctgcaa agactccatt ctggaagcca tcgacgcagg catcaaactg attatcacca
    11401 tcactgaagg catcccgacg ctggatatgc tgactgtgaa agtgaaactg gatgaagccg
    11461 gcgttcgtat gatcggcccg aactgcccag gcgttatcac cccgggtgaa tgcaaaattg
    11521 gtatccaacc gggtcacatt cacaaaccgg gtaaagtggg tatcgtttcc cgttccggta
    11581 cactgaccta tgaagcggtt aaacagacca cggattacgg tttcggtcag tcgacctgtg
    11641 tcggtatcgg cggtgacccg atccctggct ctaactttat cgacattctc gaaatgttcg
    11701 aaaaagatcc gcagaccgaa gcgatcgtga tgatcggtga gatcggcggt agcgctgaag
    11761 aagaagctgc tgcgtacatc aaagagcacg ttaccaagcc agttgtgggt tacatcgctg
    11821 gtgtgactgc gccgaaaggc aaacgtatgg gccacgcggg tgccatcatt gccggtggga
    11881 aagggactgc ggatgagaaa ttcgctgctc tggaagccgc aggcgtgaaa accgttcgca
    11941 gcctggcgga tatcggtgaa gcactgaaaa ctgttctgaa ataaatactt gtaataagaa
    12001 atagccctcg ccgcttccct ctacaggaat ggcgaagggc tgtcggtttc gacatggttg
    12061 gccatcgtat gatggccttt tttgtgcctt aaacgttaac cacggtctaa atctgactgc
    12121 aggcttgact gcatgaccac agtcactgtg gattgccgca accgaccgca agccacgaat
    12181 aagaacgacg gcgaatttcg aatcgttacc tgatgcactg gcttcccagc gtttgaccgt
    12241 agcgttaagt aaatcaacaa aagcgatgtt caccgctttt cgtcaagtcg tgccatttgt
    12301 cagccaggat cgggtcttta ccccggatat tgcagcatcg caaaaattct tagagggata
    12361 tgctataaat caaaaataaa atctttgttg attgtttatc ataatatcca cagatatgat
    12421 aaatgatatc aatttaataa ttttcactta at
//
LOCUS       DAZFKF010000131        12329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFKF010000131 DAZFKF010000000
VERSION     DAZFKF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.133"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            357..1745
                     /gene="narU"
                     /locus_tag="ACHKA1_004525"
     CDS             357..1745
                     /gene="narU"
                     /locus_tag="ACHKA1_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO5682082.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASITDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1840..5580
                     /gene="narZ"
                     /locus_tag="ACHKA1_004526"
     CDS             1840..5580
                     /gene="narZ"
                     /locus_tag="ACHKA1_004526"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO5682083.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGELRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     KELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5577..7121
                     /gene="narH"
                     /locus_tag="ACHKA1_004527"
     CDS             5577..7121
                     /gene="narH"
                     /locus_tag="ACHKA1_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO5682084.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7121..7816
                     /gene="narW"
                     /locus_tag="ACHKA1_004528"
     CDS             7121..7816
                     /gene="narW"
                     /locus_tag="ACHKA1_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO5682085.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSILPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGEK"
     gene            7813..8493
                     /gene="narI"
                     /locus_tag="ACHKA1_004529"
     CDS             7813..8493
                     /gene="narI"
                     /locus_tag="ACHKA1_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO5682086.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLVQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8573..9466
                     /locus_tag="ACHKA1_004530"
     CDS             8573..9466
                     /locus_tag="ACHKA1_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO5682087.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDDYRISLEQGTPGFEPPLEDETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9562..10407)
                     /gene="nhoA"
                     /locus_tag="ACHKA1_004531"
     CDS             complement(9562..10407)
                     /gene="nhoA"
                     /locus_tag="ACHKA1_004531"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO5682088.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVPDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10580..11149
                     /locus_tag="ACHKA1_004532"
     CDS             10580..11149
                     /locus_tag="ACHKA1_004532"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO5682089.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIEHNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEQYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11153..11380)
                     /gene="pptA"
                     /locus_tag="ACHKA1_004533"
     CDS             complement(11153..11380)
                     /gene="pptA"
                     /locus_tag="ACHKA1_004533"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFO5682090.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISISL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11975..12328)
                     /locus_tag="ACHKA1_004534"
     CDS             complement(11975..12328)
                     /locus_tag="ACHKA1_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682091.1"
                     /translation="MISCDYYNAGMDYQDNIALEQVVTWDSDNIFTPNIFEHNGRCFM
                     KKNETWMLLNGDATPNIRTMSNKKIECLEKSEVEWASRVCDSITDKERENLKALICSK
                     PAKSVNTDKRPALGY"
BASE COUNT         2914 a         3187 c         3169 g         3059 t
ORIGIN      
        1 cagatcgttt tcctccagct catggccttt tgagtgaata aattattgct aattctataa
       61 gtgtcttgtc ttgttaagaa tatagttaat atacaaccct ttggattcat catctgaagg
      121 gttgaatcat gacatgcgta catagctgaa ccccgtttaa tggttagctt gttagttaca
      181 tttagtaaca cataactggc tgcttaatcc ccattctgcc cccgcggtta cgctttctta
      241 tctattcttt caatcagcac ttcacccaat tcatccttaa ttctaataac tggctattgc
      301 gccttacctc ctgttttgtc ttttttcatt accaatgtgt gcatgtgagg aacaatatgg
      361 cactgcaaaa tgagaaaaat agtcgttatc ttttacgcga ctggaaacca gaaaatccgg
      421 ccttctggga aaataaagga aaacatattg ctcgaagaaa cctctggata tcagtcagtt
      481 gtctacttct tgccttctgt gtctggatgc tatttagcgc agttaccgtt aatctcaata
      541 aaatcggttt taattttact accgatcaac tctttttatt aaccgcatta ccctccgttt
      601 ctggcgcatt attgcgtgtt ccctactcct ttatggtacc tatattcggt ggacgccgat
      661 ggacggtttt tagtactgca atcctgatta ttccttgcgt ctggctcgga attgccgtgc
      721 agaatccgaa tactcctttt gggatcttta tcgttatcgc tttgctatgc ggttttgcag
      781 gtgcgaactt tgcttcgagc atgggcaata tcagtttctt ctttccaaaa gccaggcaag
      841 ggagcgctct tgggattaat ggcggattag gaaacttagg tgtaagtgtg atgcagctgg
      901 ttgcaccgct ggtcattttt gtacccgtat tcgcctttct cggcgtcaat ggcgtcccgc
      961 aggccgacgg ttcggtgatg tcgctggcga atgccgcatg gatttgggta ccgctactgg
     1021 cgattgccac gatcgccgcc tggtcaggga tgaatgatat cgccagttca cgcgcgtcaa
     1081 ttaccgacca gctccctgtc ttacaacgcc tgcatctctg gctgctgagc ctgctttacc
     1141 ttgccacctt cggttcgttt atcggttttt ctgcgggttt tgccatgctg gcaaaaaccc
     1201 agttcccgga tgtgaatatt ctgcgcctgg cgttctttgg cccatttatc ggtgccatcg
     1261 cgcggtcggt tggtggtgct atttccgata aattcggcgg cgtgcgggtg acgttgatca
     1321 actttatttt tatggcgatt ttcagcgccc tgctgttcct taccttaccg ggcacaggct
     1381 ccggtaattt catcgcattt tacgccgtat ttatggggct gtttctgacc gcgggtctgg
     1441 gaagtggttc tactttccag atgatcgccg tcatctttcg ccagataacc atttatcggg
     1501 tgaagatgaa aggcggtagt gatgagcaag ctcagagaga agctatcacc gaaacggcgg
     1561 cggctctggg ctttatctca gccattggcg cagtgggcgg cttttttatt ccgcaggcat
     1621 ttggcatgtc gctcaatatg accggctctc cagtgggcgc gatgaaagtg tttttaatct
     1681 tctacatcgt ttgtgtgcta ctgacctggc tggtttatgg tcggcggaaa ttcagccaaa
     1741 aataagagat cgatgcctga cacgtcttat caggctacaa ccgtgtcgca tccgacaagg
     1801 catcagatcc caattcattc ctggagcagg agtcatgtca tgagtaaact tttggatcgc
     1861 tttcgctact tcaaacaaaa gggcgatacc tttgccgatg gtcacggaca ggtgatgcat
     1921 agcaaccgcg actgggagga cagctatcgc cagcgttggc agttcgacaa aatcgtgcgt
     1981 tccacccacg gtgttaactg tacaggctcc tgtagctgga aaatctacgt taaaaatggt
     2041 ctggtgacct gggaaatcca acagaccgac tacccgcgca ctcgccctga cctgcccaat
     2101 catgaacctc gcggctgccc gcgtggcgca agttactcct ggtatcttta cagcgctaac
     2161 cgcctgaaat acccgctcat tcgtaaacga ctgattgaac tgtggcgcga agccctcaaa
     2221 caacacagcg atccggtact tgcgtgggca tcgattatga acgatccgca aaagtgcctg
     2281 agctacaaac aagtgcgtgg gcgcggcggg tttatccgct ccaactggca ggaactaaac
     2341 cagctgattg ccgccgctaa cgtctggacc atcaaaacct acggcccgga tcgcgttgcc
     2401 ggtttctcgc cgatcccggc gatgtcgatg gtttcttacg ccgccggaac acgttatctg
     2461 tcactattag gtggcacctg tttaagcttc tacgactggt attgcgacct gccgcccgcc
     2521 tcgccgatga cctggggcga gcaaaccgac gtgccggaat ctgccgactg gtataactcc
     2581 agttacatca tcgcctgggg gtctaacgta ccgcagacac gtacgccgga cgcccatttc
     2641 tttaccgaag tacgctacaa aggcactaaa accatcgcca ttacccctga ctactctgaa
     2701 gtggccaaat tgtgcgacca gtggctggca ccaaaacaag gcactgatag cgccctggcg
     2761 atggcaatgg gccatgtgat tttaaaagag tttcatctcg ataatcccag cgactacttt
     2821 atcaactact gccgccgcta tagcgacatg ccgatgctgg taatgctgga acctcgcgac
     2881 gacggtagct acgtacccgg gcgcatgatc cgcgcgtctg acctggtaga tggactaggc
     2941 gaaagcaata atccgcagtg gaaaaccgtg gcggttaata ccgcaggtga attggtagtg
     3001 ccgaatggtt cgattggttt ccgctgggga gaaaaaggca aatggaatct ggaatccatt
     3061 gccgccggta aggaaaccga attgtcgtta accctgctcg gtcaacatga cgccgttgca
     3121 ggcgtggctt tcccctactt tggcggcatc gaaaatccgc attttcgcag cgtaaaacac
     3181 aatcctgtcc tggtgcgtca attacccgtt aaaaacctga cattagccga tggcagcacc
     3241 tgtccagtgg tcagcgttta tgatttggta ctggcgaatt acggcctcga tcgcgggctg
     3301 gaagatgaaa acagcgcgaa agattacgct gaaatcaaac cgtacacccc tgcctggggc
     3361 gagcaaatta ccggcgttcc gcgccagtac attgaaacca tcgcccgcga atttgccgat
     3421 accgctcata aaacgcatgg gcgctcgatg attatcctcg gtgctggtgt caaccactgg
     3481 tatcacatgg acatgaacta ccgcgggatg atcaacatgc tgatcttctg cggttgtgtc
     3541 ggacaaagcg gtggcggctg ggcacactat gtcggtcagg aaaaactgcg cccgcaaacc
     3601 ggctggttgc cgctggcctt tgcgctcgac tggaaccgac caccacgcca gatgaacagc
     3661 acctcgtttt tctacaatca ttccagccaa tggcgctatg aaaaagtctc tgcgcaggag
     3721 ttactttcac cgcttgccga tgccagtaag tacagcggtc atctgattga tttcaacgtt
     3781 cgcgccgaac gcatgggctg gctaccttct gcgccgcagc tggggcgtaa cccgctcggg
     3841 attaaagctg aagccgacaa ggccggatta tcccccacag aatttaccgc ccaggcgctg
     3901 aaatcgggcg agttgcgtat ggcctgcgaa caaccagata gcggcagcaa tcatccgcgt
     3961 aatttgtttg tctggcgttc taacctgctt ggctcctccg gcaaaggcca cgagtatatg
     4021 cagaagtatc tgctggggac cgaaagcggg attcagggca aggaactcgg tgccagcgac
     4081 gggatcaaac cggaagaagt cgagtggcaa actgcggcga ttgaaggcaa gctcgacctg
     4141 ctggtgacgc tcgacttccg catgtccagt acctgcctgt tctccgatat cgttctgccc
     4201 accgccacct ggtatgaaaa agacgatatg aacacctcgg atatgcatcc gtttattcat
     4261 ccgctatctg cggcggtcga tccggcctgg gaatcacgca gcgactggga aatctacaaa
     4321 ggtattgcca aagcattttc gcaagtgtgt gtgggtcatc ttggcaaaga aaccgacgtg
     4381 gtattgcaac cactgctgca cgactctccg gcagagctct cacagccgtg tgaagtgctc
     4441 gactggcgca aaggcgaatg cgatctgatt ccaggtaaaa ccgcgccgaa tattgtggcg
     4501 gtggagcgcg actaccctgc tacgtatgaa cgctttacct cgctcgggcc attgatggac
     4561 aaacttggca acggcggtaa agggatttcg tggaataccc aggatgaaat cgatttcctc
     4621 ggtaaactca attacaccaa acgtgatggc ccggcacagg ggcgtccgct gattgacacc
     4681 gccattgacg cttcagaagt gattctggca ctggcaccag aaaccaacgg tcatgttgca
     4741 gttaaagcgt ggcaggcgct gggcgagatc accggacgcg aacataccca tctggcgctg
     4801 cacaaagagg acgagaagat tcgctttcgc gatattcagg cgcagccgcg taaaattatc
     4861 tccagcccca catggtctgg tctggaaagc gatcacgtct cctataatgc gggatacacc
     4921 aacgttcatg agttaattcc gtggcgcacg ctgtcgggac gccagcagct ctatcaggat
     4981 catccgtgga tgcgtgcttt tggtgaaagc ctggtggctt atcgcccgcc tatcgacacc
     5041 cgtagcgtca gtgagatgcg ccagatcccg ccaaacggct tcccggaaaa agcacttaac
     5101 ttcctgacgc cgcaccagaa atggggcatt cactcaacct acagtgaaaa cctgctaatg
     5161 ctgacgctct ctcgcggtgg accgattgtc tggatcagcg aaaccgatgc ccgtgaacta
     5221 accattgtcg ataacgactg ggtggaagta tttaacgcca acggcgcgct gactgcccgc
     5281 gcggtggtca gccaacgtgt accgccgggc atgaccatga tgtatcacgc ccaggaacgc
     5341 attatgaata ttcctggttc ggaagtaact ggcatgcgcg gcggcattca taactcggtt
     5401 acccgcgttt gcccgaaacc aacgcatatg attggcggtt acgcgcagct ggcctggggc
     5461 tttaactact acggcaccgt cggctcgaac cgcgacgaat tcatcatgat ccgcaaaatg
     5521 aaaaacgtta actggctgga tgatgaaggt cgcgatcagg tacaggaggc gaaaaaatga
     5581 aaatccgttc gcaagtcggc atggtgctta atctcgataa atgtatcggc tgccatacct
     5641 gttcggtgac ctgtaaaaac gtctggaccg gtcgcgaagg tatggagtac gcatggttta
     5701 acaacgtcga aaccaaaccg ggcattggtt atccgaaaaa ctgggaagat caggaagagt
     5761 ggcaaggcgg ctgggtccgc gatgtgaatg gcaagatacg cccgcgtctg ggcagcaaga
     5821 tgggtgtaat aaccaaaatc ttcgccaacc cggtggtgcc gcagattgat gattactacg
     5881 aacctttcac cttcgactac gaacatttgc atagcgcacc ggaaggcaaa catatcccta
     5941 ctgctcgccc gcgttcgctg attgacggta agcggatgga caaagtgatc tgggggccaa
     6001 actgggaaga actgctgggc ggcgagtttg aaaaacgtgc ccgcgaccgc aacttcgagg
     6061 ccatgcaaaa ggagatgtac gggcagtttg aaaacacctt catgatgtac ctgccgcgcc
     6121 tgtgcgaaca ctgcctcaat cccagctgcg tggcgacctg cccaagcggc gctatctaca
     6181 aacgcgaaga agacggcatt gtgctgattg atcaggataa atgccgtggc tggcgtttgt
     6241 gcattagcgg ttgtccgtac aaaaaaatct acttcaactg gaaaagcggc aagtcagaaa
     6301 aatgtatctt ctgttaccca cgaattgagt ccggtcaacc gaccgtgtgc tcagaaacct
     6361 gcgtgggtcg catccgatat ctgggcgtgc tgctttacga cgccgaccgc attgaggaag
     6421 cggcaagcac cgagcgcgag gttgacctct atgaacgtca gtgcgaagtg ttcctcgatc
     6481 cacacgatcc ctcagtgatc gaagaagccc tgaaacaagg tattccacaa aacgtgattg
     6541 acgctgccca gcgttcgcct gtctacaaaa tggcgatgga ctggaaactg gcgctaccgt
     6601 tgcaccctga ataccgcacc ctgccgatgg tctggtacgt tcctccgctg tcaccgattc
     6661 agtcttacgc tgatgcgggc ggtttgccga aaagcgaagg cgtgctcccc gccatcgaaa
     6721 gcctgcgtat tccggtgcaa tatctcgcca atatgttgag tgccggtgat accggtccgg
     6781 tactgcgggc gctgaaacgg atgatggcga tgcgtcacta tatgcgttca caaaccgtgg
     6841 aaggcgttac tgatactcgt gccatcgacg aagtaggcct gagcgtcgcc caggtcgaag
     6901 agatgtatcg ctatctcgcc attgccaact atgaagatcg ttttgttatc ccgaccagcc
     6961 atcgggaaat ggcgggcgat gccttcgcag aacgcaacgg ctgcggtttt acctttggcg
     7021 acggttgcca cggttcggac agtaaattca acctgttcaa cagtagccgt atcgatgcca
     7081 tcaacatcac cgaagtgcgc gacaaagcgg agggcgaata atgcagatcc tcaaagtgat
     7141 cggtctgttg atggagtatc cggacgagct gttgtgggaa tgcaaggagg acgcgctggc
     7201 gttgatccgc cgcgacgcgc cgatgcttac ggatttcacc cacaacctgc ttaacgcgcc
     7261 gctgctggat aaacaggccg aatggtgcga agtgtttgac cgcgggcgca ctacgtcgct
     7321 gctgctgttc gaacatgttc atgccgagtc ccgcgatcgc ggccaggcaa tggtggacct
     7381 gctggcggag tatgaaaagg tcggcctgca gctggattgt cgggaactgc ccgattatct
     7441 accgctgtat ctggagtatt taagcatact gcctgacgat caggcgaaag aaggattgct
     7501 caatgttgcg ccgatcctcg ccctgcttgg cggtcgctta aaacaacgcg aggcaccgtg
     7561 gtacgcgttg tttgatgctc tgctgcaact ggcgggaagt acactttcaa gtgacagtgt
     7621 cacaaaacaa gtaaacagcg aagagcgcga tgacacccgc caggcgcttg atgcagtgtg
     7681 ggaagaggaa caggtgaagt ttattgaaga taacgccacg gcgtgtgaca gctcgccgct
     7741 taatcaatat cagcgacgct ttagccagga tgtcgcgccg cagtatgtcg acatcagtgc
     7801 gggaggtgag aaatgattca gtatctgaac gtcttttttt acgatatcta cccgtacatt
     7861 tgtgcgacgg tgtttttcct cggcagctgg ctgcgctacg actacgggca gtacacctgg
     7921 cgcgcctcct caagtcagat gctcgataaa cgcgggatgg tgatatggtc gaatctgttc
     7981 catatcggca ttttggggat cttcttcggg cacctgttcg gcatgttaac gccgcactgg
     8041 atgtacgcgt ggtttttgcc agtggcagcg aaacaactaa tggcgatggt gctcggtggt
     8101 atttgcggtg tgttgacgtt gattggcggc gcaggactgc tgtggcgcag gctgacaaat
     8161 cagcgcgtgc gtgccacttc cactacgccg gatatcatca tcatgagcat tttgctggtc
     8221 cagtgtctgt tggggctaag caccataccg ttttcagcac agtatcctga tggtagcgaa
     8281 atgatgaagc tggtgggttg ggcgcaaagc attgtcactt tccgtggtgg atcgtcagag
     8341 atgcttaacg gtgtagcgtt cgttttccgc ctgcatctgg tgttgggaat gaccatcttc
     8401 ctgctcttcc cgttcacccg actggtgcat gtgtggagcg cgccgtttga gtacttcacc
     8461 cgccggtatc agattgtgcg ctcgcggcga taattttcac cctggccgga tagcccttcg
     8521 ctctccggcc acttgcaggc tatgatagtt ctccagccac aggagaacgt taatgaaacc
     8581 gcaggtttac cacgtcgatg cttttacctc tcaaccattt cgcggcaatt ctgccggtgt
     8641 tgttttcccc gccgataatc ttagcgaagc gcaaatgcag cttatcgccc gcgaattagg
     8701 tcattcggaa actgcttttc tgctgcacag cgacgacagc gatgtgcgca tccgctactt
     8761 tacgccaacg gttgaagttc cgatttgtgg tcacgcgaca gtagctgcgc actatgtacg
     8821 tgcgaaggtg ttaggtttag gaaattgcac ggtctggcaa acatcgctgg caggaaaaca
     8881 tcgcgtgact atcgaaaagc accacgatga ttatcgtatt tcgctggaac aaggtacgcc
     8941 gggctttgag ccaccgctgg aagatgaaac acgtgcggcg attatcaacg cgcttcatct
     9001 cactgaggac gatattctgc caggcttgcc aattcaggtg gcaacaacag gacactcaaa
     9061 agtgatgatc ccgttgaaac cagaagtgga tatcgacgcc ctttcgcctg atcttgctgc
     9121 gctgagcgct atcagcaaac agattggctg caatggtttc ttcccgttcc agatccgccc
     9181 aggcaaaaac gaaaccgatg gtcgcatgtt ctcgcctgcg attggtattg tggaagatcc
     9241 ggtgactggc aatgccaatg gcccgatggg tgcatggttg gtacatcaca acgtattgcc
     9301 ccacgatggc aaggtgctgc gtgttaaagg ccatcagggg cgcgcattgg ggcgtgacgg
     9361 cgtgattgaa gtgacggtga caattcgtga taaccaaccg gaaaaagtca ccatttctgg
     9421 cgcggcggtg attttgtttc atgccgaatg ggcaattaaa ctctgaaacc gttgcctgat
     9481 gcgcttcgct tatcaggtct acaaccgagc taatgtatgc cggataaggc gtttacgccg
     9541 catccggcaa cctgacataa attattttcc cgcctccggg tgcgtatcaa acgccgccat
     9601 caccagcgct aactcatcca cggtaaagcc atgtttcgca tcatccacgc ccagaccaaa
     9661 ctgttcttgc atcacagcat ataatgacgc cacatccggt agatttcgct gctccaccgc
     9721 gtgcccattt tcataatggg taaaatgaaa attggtcagt gtcagcttgc cgccgtccgg
     9781 caaatggcgg cacatcagca aatgatggcg aaaatgcgac tgcggccagt gcgccgacca
     9841 gaaattgccc atcacataat cgctttgttg ctgctcgcag agatcaaaac ggtacatcga
     9901 ctgccaatgc tgatgatgat taaactgcaa cacccaatca tcaccctcct gcaacaaccg
     9961 atactctccg tgtggcgtgg tctgcacgag atcgggaact aaacgaatcg gcgcggttag
    10021 cgtctgccca ccgaaaccga catcagcaat ccatttttcc ccttccagtt caaccaacag
    10081 caaacgatgg gtgcgcggcg gtaatgctgg cggatttgat aacactaccc gccctaacaa
    10141 gctgcgaacg ttaaacccca gctcgcgtaa cacccgctca aacacgccat tctgctcaaa
    10201 acagtaaccg ccacgacggg ctatcaccag tttctcttcc agcgattgat catcaagctg
    10261 tatttccctc ggcagcaaaa cgtcgaggtt ttcaaacgga atggtgcaat tgtgtttcag
    10321 gtgcaatgca cgaagcgtat caatattgac cgcagcagct cccgaccagt taatacgggc
    10381 aaaatagtga ttcagaatgg gcgtcatcag gcgtttccct tagtggtttt ctcaacttat
    10441 aacctggaat agacgccgca ccggctattt tgtgcgatcg cggacatgca tcacgttcgc
    10501 aaatcgccaa tcccgttaat ttcgttactt attgcgttac ggactacgct taaaaaaacc
    10561 ttcttcgcga ggcgatacca tgagccgatt catccccata gaattacacc atgccagccg
    10621 tctgttaaat catggcccaa ctgtcttgat caccagtttc gatgagcaat cccagcggcg
    10681 taacattatg gctgcagcct ggtcaatgcc ggtggagttt gaacctccac gcgtggcgat
    10741 tgtggtagat aaatcgacat ggaccaggga gttgattgaa cataacggta aatttggcat
    10801 cgttatcccg ggcgttgcag caactaactg gacgtgggca gtgggaagtg tgtcggggcg
    10861 tgaagaagat aaatttaatt gctatggcat tccggttgtg agaggcccgg tatttggttt
    10921 gcctctggtc gaggaaaaat gtctggcgtg gatggagtgt cgcctgctac ctgcgacttc
    10981 tgcgcaagaa caatacgaca cactgtttgg cgaggtagta tcagcagcgg cagacgcacg
    11041 ggtatttgtc gaaggccgct ggcagtttga tgatgataag ctcaatacgt tgcatcattt
    11101 aggtgctggc acgtttgtta ctagcggcaa gcgtgttacg gcgggttaag cattacatgc
    11161 tgtaaccagg tttctttatc aaagcctcca tttggggcgc gatttcggca tcccagatag
    11221 cttgccatga ttctggttga atctgctgta gagaaatgct tatcgagcta tctttactgt
    11281 tcagatggcg aataataacg tcggtaatat ctgcagcaag tgctgctttt tgttgttcgt
    11341 ccagttcacg cggaaaacat ttaatgtcga tgtgcggcat gattgtgctc cttttgaaag
    11401 atgtcagtaa aattaacaca cattgtgaat gtcgtatgtc cggtgatgcg tttttacgac
    11461 aataatgcct acttgaaatt gtagatattt gcactacacc cttttaaata aagcaccaac
    11521 aaatcgtcaa cttgcagaac atattcttct gcctacatac cattaagaaa acatcaagtt
    11581 cccgacttgc caatttatta acgcccatta tagacgctga aacaagctac ggacaaagga
    11641 gatgccaaaa ttaccggcaa tgcagaaaaa ctcaacgaac catggctctt taccacagca
    11701 gccggggttg tttgggaccg acttccgaca gcagatcccg gtgggcctgc caccatctct
    11761 tcacaagctt atgcttatta catgcaaaac gtcccgtttt gcatctttgt gcctacggca
    11821 cacagtgatc tttccctggt ccctgtcccg gattgtagta ccggtgccag ttgtagtgca
    11881 gttcggattc gtcgtcatac tgctagcccg gatggcggag cagttgctct agtcactcag
    11941 aatattgtaa tagtgataga gtgcagtgcc ggtttcaata tcctaacgct ggtcttttgt
    12001 ctgtattaac tgatttagct ggtttgctac atattaatgc cttcagattt tccctttcct
    12061 tgtctgtgat actgtcgcaa accctactag cccattctac ttcactcttc tccaggcatt
    12121 ctattttctt attgctcatt gttctaatgt ttggtgttgc atctccattt aataacatcc
    12181 atgtttcatt tttcttcata aaacatcttc cattatgttc aaagatatta ggtgtgaata
    12241 tattatcaga atcccatgtt acgacttgct cgagggctat attatcttga tagtccatgc
    12301 cagcattata ataatcacaa gatataatt
//
LOCUS       DAZFKF010000132        12076 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFKF010000132 DAZFKF010000000
VERSION     DAZFKF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.134"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            91..996
                     /locus_tag="ACHKA1_004535"
     CDS             91..996
                     /locus_tag="ACHKA1_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5682092.1"
                     /translation="MRGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLRTPLFSHSKKRIFLNDAGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFDEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(1030..1632)
                     /gene="cobO"
                     /locus_tag="ACHKA1_004536"
     CDS             complement(1030..1632)
                     /gene="cobO"
                     /locus_tag="ACHKA1_004536"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO5682093.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(1642..3294)
                     /locus_tag="ACHKA1_004537"
     CDS             complement(1642..3294)
                     /locus_tag="ACHKA1_004537"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5682094.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDPQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIIASKGQQIWTGGNDAEALSKGIYTTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDICAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKIAKLASTKYYDNLPTSGNEQGQAFRDIELEKALL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMLDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGESMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYVDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(3402..4682)
                     /locus_tag="ACHKA1_004538"
     CDS             complement(3402..4682)
                     /locus_tag="ACHKA1_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFO5682095.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVAAEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(4843..5160)
                     /locus_tag="ACHKA1_004539"
     CDS             complement(4843..5160)
                     /locus_tag="ACHKA1_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HFO5682096.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(5157..6527)
                     /locus_tag="ACHKA1_004540"
     CDS             complement(5157..6527)
                     /locus_tag="ACHKA1_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HFO5682097.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AQEEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKEGAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAEIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(6531..7772)
                     /locus_tag="ACHKA1_004541"
     CDS             complement(6531..7772)
                     /locus_tag="ACHKA1_004541"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HFO5682098.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     TVRQAGECVSVQLILENGAVAVGDCAAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(7772..9217)
                     /locus_tag="ACHKA1_004542"
     CDS             complement(7772..9217)
                     /locus_tag="ACHKA1_004542"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HFO5682099.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNAGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(9236..10624)
                     /gene="glmL"
                     /locus_tag="ACHKA1_004543"
     CDS             complement(9236..10624)
                     /gene="glmL"
                     /locus_tag="ACHKA1_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFO5682100.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(10624..11073)
                     /gene="glmS"
                     /locus_tag="ACHKA1_004544"
     CDS             complement(10624..11073)
                     /gene="glmS"
                     /locus_tag="ACHKA1_004544"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFO5682101.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVENKFKEMGFDRVFAPSHDLEEVCQLMAHDINQRHDVDTRILEEAI"
     gene            complement(11621..12043)
                     /locus_tag="ACHKA1_004545"
     CDS             complement(11621..12043)
                     /locus_tag="ACHKA1_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682102.1"
                     /translation="MIPSQISFNTLPVNATYASEPTVDTQKFSDILYSAGCSLKDLAY
                     LSRCLASIHPTIAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEINSDT
                     VIFSILSSLIDSAISKFQHQIPVNGSVNRELLYEDYTD"
BASE COUNT         2925 a         2936 c         2993 g         3222 t
ORIGIN      
        1 tataaattat ttattgctgc tttggttacc attacttcgt aataagtaat gtatttttga
       61 tggtttaaac tatttttaag ggacggcatt atgcgaggga aaatacctaa aacggaactg
      121 ctggtgactt ttgaggtcgt cgcacgtcat gaaagttata ccagagcggc agaggagtta
      181 gcgttaaccc aaagtgcagt attcagacaa gtaagtgcgc tggaggaatt tttacgtact
      241 ccgttatttt cacactctaa aaaacgcatt tttcttaatg atgcagggaa gtattacctg
      301 gggatagtaa aagaaacatt aaataagctg gaacgcgata ccaataccat tatgacctgg
      361 cagcctacgg ttcaggttat tgaacttgcc gttaatccta cttttagtac ccactggttg
      421 atcccaaatt tacatgaatt cacaaagtta catcccgata ttattgttaa tattcattct
      481 ttagctaata acggcgattt tcttaatcgg gagtacgatg ctgtcattat gcgcgaaaat
      541 ttttgcgcgc cctgggcgga agtagaatat ctctttgatg aggagatttt gccggtatgc
      601 agcggcagtt tgcttgcaat gtccgatcaa aagctgtcag tggcagaact gcttactgag
      661 ttaccgcttc tgcatcaaag cacccgtatt actggctggg aagagtggtt tgctttatct
      721 ggcgttagca gtccgttggt caataacgga ccacgtttcg atttgctttc aatgctgatt
      781 gccgccgtgc gctctaatct cggcgtggcg ctgttaccac gtttcgctat tcaacatgat
      841 ttggatagtg gcgatatggt gatcccctgt gatgtgccta ttcgtaccgg taatcgtttt
      901 attatgacgt ggcaggaaga gaagtctgac tcaccgcact tgcagcaatt tcgtgaatgg
      961 ttgctggcga aatcggtcgt accgcaggag atgtaaaacc tgcgcctggc gggatgccag
     1021 gcgcagttat taccagtcga tacccggctg ggcctgtatg ccgttatcaa aagcatgttt
     1081 gacggggcgg atttcgctga ccgtatctgc catttttaat atctgactgt ggcaaccgcg
     1141 tccggtgaca atgacacttt gttgtgccgg gcggttttgc agagaagcga tcacttcttc
     1201 ggtatccaga taatggtaag ccagcatata ggtcagctca tcaagcacca ctaagtcgta
     1261 acgcttgtca gccagcatcc gtttgctttc cgaccagacc tctttcgccg cgtcgatatc
     1321 cgcttgccgg ttctgtgtct cccaggtaaa accggttccc ataatatgaa actcaacccc
     1381 cagcggctgg agaaggttgt attcgccgtt gtcccattgc cccttgatgt actgcgcaac
     1441 ccccaccgtt ttgccatgac ccaccgcgcg cgtcacagta ccaaacgcgg cagtggattt
     1501 ccctttgccg ttgccggtga aaacaatcaa gatccctttt ttctctatcg ccgctgcgac
     1561 gcgcgtatcg acctgttcct tcaatttctg ttggcgctgc acatgccgtt cagttgaaat
     1621 tcgcgcttcc attcattttc cttaatgtcc cgcagggcaa ctggcgcagc ggtgttgctg
     1681 ctcaaactga ctaaagaaat tattgccttt atcatctacc aggatgaatg cgggcagatt
     1741 ttccacttcc atcatccaca ccgcttccat acccagctcc gggtattcca ggcaacgcag
     1801 acttttgaca tattcttgcg ccagtaacgc agctgcgccg ccaatacttc ccagattgaa
     1861 gccgccatgt ttgtgacagg catcggtgac ctgctggctg cggttacctt tcgataacat
     1921 caccagactg ccgccggccg cctggaaggt atcgacataa ccgtccattc gaccgcccgt
     1981 ggttggaccc agtgaaccgc aggccatgtt ctccggtgtt ttcgccgggc cagcgtaata
     2041 gacaatatga tgcttcaggt actccggcat cgattcgccg ctatccagcc gcgcttttat
     2101 tttggcgtgg gcgatatcgc gcgccactac gatgggacca cttaacgaca cgcgggtgcc
     2161 taccggtaat ctggcgagat ccagcatcac gtcacgcaac ggacggttta gatccagttg
     2221 tacatgttgc gcgtgatttt cttcccgcaa tgaggctgga atatattgcc ccggattgtg
     2281 ttcgagtttt tccagccaga taccgtgttt gttaattttt gctttgatat tgcgatcggc
     2341 agaacaggag agcgccatag ctatcgggca agagccaccg tgacgcggca gacgaatgac
     2401 gcgaatatca tgagcgaaat atttcccccc aaattgtgca ccaataccaa attgctgact
     2461 ggcctcgagc aacgcttttt ccagctcaat atcacggaat gcttgtccct gctcgtttcc
     2521 gctggtgggc aaattgtcgt aatatttcgt tgaggccagc ttggcaattt ttaaggtttg
     2581 atcggctgaa agccctccga cgacaaaggc gatgtggtaa ggcgggcagg cagcggtgcc
     2641 cagcgatttc attttttcga ttaaaaatgc ggtcagtttt tctggttgca ataaggattt
     2701 ggtttcctga taaagcgcgg ctttattggc agagccgcct cctttattaa cacagaggaa
     2761 atggtactca tcaccagcga cggcgcagat atcaatctgc gccgggagat tggtttgcgt
     2821 attgacttcg gtatacatat ccagcggcgc attttgtgaa aagcgcagat tattttcctg
     2881 aaaagtggtg taaatgcctt tgcttaaggc ttcggcgtcg ttacctccgg tccatatctg
     2941 ttgcccttta ctggcaataa tagttgcggt gccggtatcc tgacagttgg gtaatacccc
     3001 ttttgctgag acttcagcat tacgtaataa ctgcaacgca acgtatttat cattactact
     3061 ggcctgcgga tcgttaagaa tgctggcgac ctgttgcaaa tgagcggaac gtaagaaaaa
     3121 agaggcttca taaaatgcct gccgggcaag cagcgttaat gcttcagggg cgatttttat
     3181 cacttcttcg ccgtctaatt ccgttactgt aatatgctga tcgctaatta atgtatattc
     3241 tgtaccgtct ttattttgta gaaacggttc ttgccagata aatggtttgg acatatttca
     3301 cttttcacct taaacgttag tgttacttaa ttgccgtcta acttcatgat ccggcttata
     3361 aaaatgcgta gtcattggct tcagattgct taagagcccc ctcagggcag agcaacgttc
     3421 gattcattgg gttttaatgc tttaccccga cagccctcgc cgaaatgttc gccttcccac
     3481 cgactcacca tcgcagcgcc cattgcgttg ctcatgacgt ttgtcgcgga acgccccata
     3541 tcaaggaacg gatcaacgcc catcagcaag atcagccccg cttccggaat attgaactgg
     3601 ttgagcgtgg cggcgataac caccattgag gcgcgtggta cgccagccat tcctttcgag
     3661 gtcaacatca ggatcaacag catggtgatt tgctcaccga tggataaatg gatattgcag
     3721 gcctgggcga tgaaaactgt ggcgaaggag cagtaggcca ttgatccaac gagattaaat
     3781 gagtagccaa tgggtaagac aaagctggca attttggggg aaacgccaaa ttgctccagt
     3841 ttttcaagcg ttcccggaaa agccgcttca gaactggatg tggtaaatgc cagcagggcg
     3901 ggttccgaaa gggcgcgggt caggcgtctg atgcaggggc cgacataaac gatggccaga
     3961 ccgataagca gcacccaaag taataacatg gtgaaataaa attcacccat aaagatcccg
     4021 gcgctcacca taactgccag tcctcgttca gcaatcaatg ctgaaatagc agcgaatacg
     4081 gtcaggggag cgaagagcat gacgtagcca gtgagcttta acatggcatg tgccagcgaa
     4141 tctaaggcgt gaacgatggc gctgcctttc tcgccaatcg ccgtcaggct acagccgagg
     4201 aaaattgaga acaccacgat ttgcaaaatt tcattgtgcg ccatggcatc gacaatgctg
     4261 gtggggaaag catgcgaaat aaatactttt agcgtgaagg gttctgccgc gaccactccg
     4321 gtggtttcgg caccgtgtgc aacaaaatta attcctgtac ctggcatgaa gaaatttact
     4381 gttatcaagc ctaaggcaat tgacagcaat gaggcgcaaa taaataaaaa gagtgtttta
     4441 gaaaaaatac gaccaagggc tttggcatcg cccattttag caatacctac caccagggta
     4501 gaaaccacta acggagcgat aatcattttt atcagtcgta agaaaatcgt cgtgaatatc
     4561 gatatttctt gcgcatagag ttttgctgtt tccggtgaag cagtgttatt gagcactacg
     4621 ccgataatca ttccaagtac cagcgccaaa agaatcatcg tggttaaact tattttcttc
     4681 attttttcct ccatcaggtg cacggcgata aatcagaatc aggagaagta gatccctgaa
     4741 tagagattca ggcatctacc tgctattaca accttacaaa tagctgaagt gttgaactgc
     4801 aataagtgac gggatgtcag ttggctaaaa gtattcggat ttttacaaat cgaaactcat
     4861 atttaataaa ggtgcatgtt gctgtgcacc atagacatca ttgtcaccta cattcccgga
     4921 gataatatct cgtggcatga cgatttttac tgcgttagaa ggatcaaacc agacaatacg
     4981 atgaataaag tcaggttcaa catgatataa gcgcgcaatc agttctgggg ttaattgccc
     5041 tgatgattta actcgctcat agttttcttt cgttttaaat aaaatatcta aaaccagttc
     5101 gtaaggtccg gcatttttcg aacgaatgac ttgcgccagt gagacaattg actgtttcat
     5161 gcctgaactc cttctggcgt cacctgttca atatggaaat caaaacgcag agcgtcgttg
     5221 gcttcaatca ggtgatatat ggaaaattcg tataccggcc cgccctggat atcagaaggg
     5281 gagaacggga acgccagatt gcctgcggta gcgatgcggt tttcatagcc gtagtgcagc
     5341 atggtagagc gcaccagcga gcaaacgcta ttggcaattt cctgggtcgg tgcaactaca
     5401 tcaaggacaa tccccagctc atgcccggca gtttgcatcg gttcatggtc tcccatcacc
     5461 ccgtttttgc cgtacaggtg gaaattgatg cgaatgctgt catcgtcgag cgacaggtta
     5521 cgcgaaacgc tggttttgac ttcatcgatg attttatcga tctcggcgat cataatcggg
     5581 tcgcgcgttc cggcgatggt cagacagcgg aagcccaccc gtcgtgcacc ttccagtttc
     5641 agggcatacg gcgtttcttc atgcttagaa ccgctgacgt agacttcgcc gtcattgact
     5701 gctttaaatg tgcagccttt caggttcaac acgccgccag gtcctggcag gaagtaggga
     5761 tcggatttct catacagtgt gtgtgcagct gctgacgttt cggtaaattt acgcttcgga
     5821 ttaaatgtct tcagcgtaaa gccgttgtca tcaatgatgc ccatcgcaca gtcagagcct
     5881 gagccgggcg ttgcggcgat tgccgcgcat tcaaggattt tcccacaatg cagcgccaga
     5941 ccttcatcaa agccttgcat gatcgggagc gcagcaaagc aggccgggtc gtaagcgcgc
     6001 ccgcccagca ccacttgcgc gccttctttc agcgcccgct ggaagggttc gatgcccatt
     6061 tgcgcgacga tatacgtact ttcgtcaatc gcgtcgtggg ttaacggcgg gacaaaatcc
     6121 agcgccgtga ttttgccgtt atccagcgcc tggtggacga tggctttgtc aacgtctgcc
     6181 gggatcaacg ccatcgagaa cgagaggtgc tcttcctggg caatctcatg aattatttct
     6241 cggcaccact ccaggtgtgg agcggcacca gaaccgccgg ccgttccgat caccaccggt
     6301 atattatttt gcacgcctgc ggtgatcata tagcgcagat cgcgcttgac ccctgcgcga
     6361 tcggtaaacg gtttgcctgc cccgaggtag tgggggcctg gatcggaaga gcctgcatca
     6421 acggcgatca gatcgggtga ttctgccatc gctttgcgga agctctcttc cgggaatcca
     6481 tacccgagga ttgctgttgg cgataagact ttaaaagaac gagccatttg ttaatcctta
     6541 gcctgcaaca gcgcgatagt acgattcatt tcgttgaaga cgatatcgag gccttcgtca
     6601 aagcccatcc ctggctttac cagcatacgc atagggcgag cggcaagggc gacgtggaca
     6661 caggtgcggg cactgacatc agtttcattg caggtgccgc cctggtacgc ttccatgctg
     6721 tggctgttgc agtaaagaac cgcatcgacg atgttgtgaa tactgcccag atccggggtt
     6781 ttgatttgca ccatgtgaca actggcggca tcagtgaaat caacaatatc ctggtaggtg
     6841 ttacaccatt catcggccac aattttcacg ccggaaccga ggcgcgtcag ctctttggta
     6901 atcgcggtca gcaggcgaat ttgatcgggc ttgttaccgg catcgaccgg cccttcaatg
     6961 tagagcggca ggccttgcgc ttctttttcc aggctggcga tgtattgcgc acagcgaagc
     7021 ggatccatat cgaagatcag accgatagtg ccgtatacat cgatgtgcag ggtagggtgg
     7081 tagcgtgcgc tggtgcgctt gcttagaatg cgatccgaca accagcggac atattcgcgc
     7141 agtttttcac ctttaaagcc cagcttctct tcgacgttat taatcagcgc atggggcagc
     7201 acgtcgatgc ccttgaggat catcttatcg acggcgatat atcgatcgtc gccgctctga
     7261 ccaaataatg gaatggattc cgccacgcgt ggcaactgcc attcatcaca gaccacttca
     7321 gttttcagac ggccggttgc cagcgcggta gcatcaagca gcgcctgtga taatccgtag
     7381 cgcacggcgg tatgcagcaa gttgccgtca atacgcaatt tgtcgaagaa acgggcattc
     7441 ggcaggaaag catccacatc gcggcctacc agtaatggct tgatatggtc gttgaggaac
     7501 ggaataaagt gctctgcgag gaacagtgga tcgcgaccac ctgccccaga atactgtacg
     7561 gcagcacagt cacctacagc gaccgcgccg ttttccagaa tcaactgtac cgatacgcac
     7621 tccccggcct gacgtacggt gttaaacccc tgtgttactg gctccccggt ataaaaaaag
     7681 ccgtcgtgac ccgctccgtt ttttatcgcc tgctggtcat cgaaatagaa tgaggaatag
     7741 ccagcggtga acagggcctg ttttattttc attatggtct tcctattaat ttactgtggg
     7801 atacagcatt gatgtcatct acaaccatct ggaatgtcgg ttggcgtcct tcacagcgtg
     7861 cgcgttccgc aacgtaatcg tggtggaggt cgagaatatc ttttggcaat ggaacggctc
     7921 cggcttccag tacgcgtatc gcgccggcgt tgtcacggac cggaagaatt ttgcccgcgt
     7981 tgcaggcggc tggcgcaaac ggcacatcca gtacgcccgc ttcaaatgcc agcaccgttc
     8041 cgcgcgcaat atcaccatta cccagttcga acactttatt cagtactgcg cgaacctcgc
     8101 tcttaatcag ttcaatttcc agttcaaccg cagggcacgg cgggaatttc tgctcattga
     8161 ccatgttgag catctggcgg gaggctttca gcccctgaat gttggcagcg gcagtcggga
     8221 tgccccaggc ttcgtgcgga cttttggtga tgactttggt ggcaccagac atccccgcga
     8281 cggctgcgcc ccaggagata atagcgaacg ctttcgattc gtcttccgga aagccgccca
     8341 tccactggtg aaacacggta ctcaattcat agtcggtata gccatagctc tgaaaatatt
     8401 cgtgtgcaag ttcacgcaga gactggatcg ccgcaacatc ctgcgtcagg ctgccgacct
     8461 ggccataacc aacggtgata gatttcacac cctgttccag tgccagcaag ccttcgatta
     8521 tcgcgataga gtgcgagata aacggcggga tcagcgtacc cgttagtggg ccaaacggtt
     8581 cgcggttgat gcggatcccg tgttcttcat acatccccat taaacggtcg cagtactgcc
     8641 agtcgcggat cgatttttcc agcgttacgc gtttggcata cggaatgttg taggaaatgc
     8701 caccgccttc ataactggta aagccactgg ccatcgagat ttcagccagc agacgcgcat
     8761 ctggcgtgcc gtggcggatc tgtagcggtt tttgcagggt ttcggtgagg cgacggcaag
     8821 ctgcgacacc atggttaact accggtaaac cgttcagttt agaggttccg gcttcgatgg
     8881 attttttaat ccctacagcc gcttcttcgt accgattcag acgggtatat gcatcgatgg
     8941 tgcttggcag cagatcgcac tcttcttgta gtgttttcag cagttcgatg tgttcgtcca
     9001 tcagcgccac acctgcgcgc ggttggctaa gggttttacc ttccttgtct gctttcagca
     9061 gcgcaagaga gaagcgttta tgctcaggga tggtttgttg gtatttaacg ccatcttcaa
     9121 agttttcaac atccttgccg gtttcccagg ttttcagtac ctgttgccgc tcggtcatga
     9181 attcgtcatg ggttaatttc ttatttcgaa gttccattga ttgcagcctt aattgttata
     9241 gggttaagca ttgaatactg gtgcgcgcgg cggccagcgg atcgactctg gctacgtttg
     9301 ccagcagtgg taatagcccc cgggaatcgc ggtaatattc aaattcggtc ggtaagagaa
     9361 tgcgtttacc gtcgtcgttt aactcgcggt atttcaacca gtgatgcata tcgaactgac
     9421 tggcacgcga gagccatcca ccggaaccca cgactttacg cacggttgtc aggtcacgtc
     9481 ccatctgtaa atcgacattg ccgacgcagg tgcagacctc ttttttggtg cccgcatgtc
     9541 tttcggtggc ataacccaca cacagtccgg ccaggacggt atcgaaatat ttctcttctg
     9601 catttgcggg taagtagtcg ggatgagcga ctagatgacg gaggtaatga taaaaagcgt
     9661 ccagctgtgc gggttgctga gcaaaattca cattgactaa ctctttcgca ctttcaccaa
     9721 ccaccactgc cgagacgcgc attcccagat cgccttccac cgtccgtttt acgaagggtt
     9781 ccggtacgcc gtgtaaaacg gtgtcgggcg agagcgtgtt ggcacaggcg gaataaacat
     9841 cggtggttgc gccgcccatg tcgatcagca tgaactcttt ccagctgtga tcgacgttac
     9901 tgattgcctt gaccagttca aagaccgtcc atggtgtcgg cattggttct tcgcctgttt
     9961 tatcaacaat gacatccagc cctttccctt taacgatgcg tttaaggaat atgtcgcaaa
    10021 tagcctggcg tgcggctaaa gggttcggat ggtcaagatc gggcagaacg ttgtcgacga
    10081 tggtcaggtc tttatgcccg aggatctcct gcacctcgtc ctgaatatcc cggtttccgg
    10141 cataaataat ggcgcagtcg agtttggatt ctgccagcac acgggcgtta ttcagtccgt
    10201 agctttcttc tccgccgtcg gtgccgccgg tgaacaagag aatatcgggc tgcgtttctt
    10261 ccagtgcctg gatatcacgg cggttcagct tgtaggcgta atattgcgca attttagcac
    10321 ccgcagagtg agcggttact ttcgcggttt ccagggtgat agaaggcacc aatcccattg
    10381 cggcaaccgc cagcccacct ttggcagagg aggaatactt caatgcaacc tcaccgctgt
    10441 ttaacagggg gagggcatta tctacgttta acacctggtt caggctggag aaaaacccct
    10501 tcgccagatg atgggtggtg gttggggtca gtacgtggtt aaccaacgtt aacgcgtccc
    10561 cctcccgggt aaagagggct gctttggtcc acgtcgatcc aatatcgaca gagacgattt
    10621 gcatcagata gcctcttcaa gaatacgagt atcaacgtca tgacgttggt tgatatcgtg
    10681 ggccatcagc tgacaaacct cttctaagtc atgacttggg gcaaatacgc gatcaaatcc
    10741 catctctttg aacttgtttt ccacgtcagc gaagtcatgt tttcctacca ccaggttacc
    10801 gcccacatag agcaggatgt cccccagtcc acgctcgatg cagcgttcgc gcatgcccag
    10861 acagtcgata tcgccatgcc catagataga ggagacgaca atcgcgtcag cgccagtttc
    10921 aatggcagca tcaatatatt cgtcctggct gaccattacg cccagattaa tgactctgaa
    10981 gtcgtggttg ctaaaaacgc ggtccagaac tttattgcct actgcatggc agtccgcgcc
    11041 aataacgcca atcacaagtg ttgctttctt catgggtaat cctttgtaag acagagagta
    11101 aaataagtta cacacgtcgt atttatggcc gttcataacg gcacaggttt aatatcacag
    11161 gagtgtcttt atctgaccgt aatgttgcaa ttgatctatt ttcaaaatgt ctttcgggaa
    11221 atgattgttt aaagtgtgtt tttaaatttc catgttaatt cagtgtgtta tgtcaactaa
    11281 tgtcatgagt gttaaatttt taaaattaac ttgatttaat gtattgagat aattgtgatc
    11341 tacttctaat ttgcattttt gtgacgtggt tttagccatg agtaaatgca tttcataaga
    11401 tttaagtcga atttcactgc aattcatctc tgcttaatgt aaaaataatg ttgaaaataa
    11461 caaatggtta atgattcatg cgaattatga ccttagttag ctcttttgat ttttcgtaaa
    11521 aaagagaggt acatgaaggt gctatcagta tgagtaacgt taatcaaagg ttttattacg
    11581 tctggcaata acgcaggcca tttttcgcga cagggtggat ttaatcagta taatcctcat
    11641 acaatagttc acgattaact gaaccgttaa cggggatttg gtgttgaaat ttagaaatgg
    11701 cggagtcaat taatgaagac aatatcgaaa aaatgactgt gtcagaatta atttcaatta
    11761 atccttctcg ttgttgacca aaaaagatat ttatcttgat ttcatttgta ctacggcaat
    11821 caatatgtag taatttttga tcgctgagat tttctgtttc ataaatgttt ttggcgatag
    11881 tcggatgtat tgatgccaga catctgctca gataagccag atcctttagg gaacaccccg
    11941 ctgagtagag gatgtcagag aatttttgtg tgtcaactgt tggctctgaa gcgtatgttg
    12001 cattgacagg aagagtattg aaagagatct gagatggtat cattatttaa atctttccgt
    12061 gagtaataaa aattaa
//
LOCUS       DAZFKF010000133        12068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFKF010000133 DAZFKF010000000
VERSION     DAZFKF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.135"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..275)
                     /locus_tag="ACHKA1_004546"
     CDS             complement(<1..275)
                     /locus_tag="ACHKA1_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5682103.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMA"
     gene            complement(485..1639)
                     /gene="ompC"
                     /locus_tag="ACHKA1_004547"
     CDS             complement(485..1639)
                     /gene="ompC"
                     /locus_tag="ACHKA1_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO5682104.1"
                     /translation="MKKMTVALSAVAVAVMFAAGAQAAEVYNKDGNKLDLYGRATALH
                     YFSDDKGNDGDQTYARLGFKGETQINDQLTGFGQWEYQFSGNKTESEGSAGNKTRLAF
                     AGLRFADVGTIDYGRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNT
                     DFFGLVDGLNFAAQYQGKNERDDLQKANGDGYGFSASYEFDGFGFVAAYTKSDRTDKQ
                     VQGLNGTADVLDPESGKKVGEKTVDSGSVAKGKHAEFWGTGLKYDANNLYLAAVYSET
                     QNMTTFGDQGVADKAQNIEAVVQYQFDFGLRPSLAYLQSRGQDVMVGGVNHGDQDLVK
                     YIDVGATYYFNKNMSTYVDYKINLIDESEFTRKAGVATDNIVAVGMTYQF"
     gene            complement(1838..2179)
                     /locus_tag="ACHKA1_004548"
     CDS             complement(1838..2179)
                     /locus_tag="ACHKA1_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5682105.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVVTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVR"
     gene            complement(2172..5414)
                     /locus_tag="ACHKA1_004549"
     CDS             complement(2172..5414)
                     /locus_tag="ACHKA1_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5682106.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASAAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGILRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGALNGVAGNIDTVATAAGVLVAVGVARYFGNLASGAMSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAVTQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEERTREALTEQNRLIDEQASRVKSLQEKIAGYQYVLSNP
                     GWTTGNGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEERR
                     VVLIRQQAAEQNKAYQSLLIMNGQHTEFNRLLGLGNELLQQRQGLASVPLRLPQATLD
                     DKQQGALNNTERQLALSRLKGEEKERARLGYAADDLGLVGDTYQEARQRYIRNSMEAW
                     RNNEANKPKSRAGKSETEKAEDSFSRLLKQQKEQLALAGKNTELAKLKYQTSQGELKT
                     LTEIQKQELLRNAALIDQKKIREQLRAREEALKNDNADERASNDAELLGYGQGERVRE
                     RMRELQQIRDGYRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQEAFYAES
                     DAQRADWQKGLQEGLSNWVDSASDYASQAAQLATDGISGMVNNITEMLNGNKVEWRSW
                     AASILQEISKVLMNAAIVNGIKMAANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGTLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            complement(5478..5891)
                     /locus_tag="ACHKA1_004550"
     CDS             complement(5478..5891)
                     /locus_tag="ACHKA1_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682107.1"
                     /translation="MKKFMLLIFSVSFFLTGCATIVGDKTQTVQVNSNPSGADFIIKD
                     ESGKTISSGKTPQNVTLEKSDGSYFGKKSYQITFSKENYQPVTLPIKASANGWYIGGN
                     FVFGGIVGWLLVDPFNGGMYTLSPESLNPGLVPKT"
     gene            complement(5955..6164)
                     /locus_tag="ACHKA1_004551"
     CDS             complement(5955..6164)
                     /locus_tag="ACHKA1_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO5682108.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEADPLAALEDALLFASEN"
     gene            complement(6260..6634)
                     /locus_tag="ACHKA1_004552"
     CDS             complement(6260..6634)
                     /locus_tag="ACHKA1_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO5682109.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QDVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(6649..7365)
                     /locus_tag="ACHKA1_004553"
     CDS             complement(6649..7365)
                     /locus_tag="ACHKA1_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO5682110.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(7431..7775)
                     /locus_tag="ACHKA1_004554"
     CDS             complement(7431..7775)
                     /locus_tag="ACHKA1_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO5682111.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVAVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(7772..8218)
                     /locus_tag="ACHKA1_004555"
     CDS             complement(7772..8218)
                     /locus_tag="ACHKA1_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO5682112.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGIESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(8215..8565)
                     /locus_tag="ACHKA1_004556"
     CDS             complement(8215..8565)
                     /locus_tag="ACHKA1_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO5682113.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(8575..8901)
                     /locus_tag="ACHKA1_004557"
     CDS             complement(8575..8901)
                     /locus_tag="ACHKA1_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO5682114.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(8898..11320)
                     /locus_tag="ACHKA1_004558"
                     /pseudo
     CDS             complement(8898..11320)
                     /locus_tag="ACHKA1_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(11266..11487)
                     /locus_tag="ACHKA1_004559"
     CDS             complement(11266..11487)
                     /locus_tag="ACHKA1_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682115.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVQRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(11532..>12068)
                     /locus_tag="ACHKA1_004560"
     CDS             complement(11532..>12068)
                     /locus_tag="ACHKA1_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5682116.1"
                     /translation="AVADVSPASITHDVKGTASSGNPDADAEAAFGQFVAANLQPTGA
                     VWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAPDIY
                     LADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWRRRR
                     TAAVAVITGVNYGSASGG"
BASE COUNT         2665 a         3826 c         2758 g         2819 t
ORIGIN      
        1 gccatcccgg tgaccagccc gaaaagattg gacactgtca gcgacgggcg ggcactgctg
       61 ccctttccgt tcatctcaaa gccgctgccc tcaatcgggt acgcctgata ttcacgcccc
      121 tgccaggtca ccggctcccc tttttcattc agctcattgc agaaaaaata ccgctcaccg
      181 ccctgcaccg tcaggtcaat ttcccagagc accacccgtg gtgactgctc tgacttaacc
      241 gactcgttca ggctttcttc gtgaatatcc tgcatatatc cgcccataaa aaaggggcgc
      301 agacgcgccc cgaacaataa aagtccaatt taaaataaaa ccaataaagg taataacaga
      361 aagttaaatc agcacatgcc agatggcaat tgctgatcgc agtgaccaat gaaaactgtg
      421 ttttcattga tacaggccgc ctgtaagggc agacggcccg tatttattaa cttatcagca
      481 ataatcagaa ctgataagtc atacccacag caacgatatt atctgtcgca acaccggctt
      541 tccgggtaaa ttcactttca tcaatcaggt taattttata atcaacatag gtggacatat
      601 tcttgttaaa gtaataagtc gcaccgacat caatatattt aaccagatcc tgatcgccat
      661 ggttcacacc accaaccata acatcctgtc cgcgggactg aaggtaggca agggacgggc
      721 gcagaccaaa atcaaactga tactgaacaa cggcttcgat attctgagct ttatccgcaa
      781 caccttgatc accaaaggtc gtcatattct gggtttcaga atacactgct gccagataca
      841 ggttattggc atcatattta agaccagttc cccagaattc agcatgttta ccttttgcca
      901 cactacctga gtcaactgtt ttttctccaa cctttttacc tgattcagga tcaagaacat
      961 ctgcagtacc gttcaacccc tgaacctgct tatcggtacg atctgacttg gtatacgcag
     1021 cgacaaaacc aaaaccatca aactcatagc tggccgagaa accatacccg tcgccattag
     1081 ccttctgaag gtcatcacgc tcatttttgc cctgatactg cgccgcaaaa ttcagaccat
     1141 caaccagtcc gaagaagtct gtattacggt aagtcaggac accggaagtt cgggccgtca
     1201 taaagttatc ggtctgcgtc cagccatcac caccaaactc aggcagtacg tcagtatatg
     1261 atccgacatc gtaagcaata ccatagttac gtccgtaatc aatggtacca acatctgcaa
     1321 atctcagacc tgcaaatgcc agacgggttt tatttcccgc ggaaccttca gattccgttt
     1381 tgttaccaga gaactggtat tcccactgac caaatccggt cagttgatca ttaatctgcg
     1441 tttcgccttt aaagccgaga cgagcataag tctggtctcc gtcattacct ttatcatccg
     1501 agaagtaatg cagagcggtt gcacgcccgt aaagatccag tttattacca tctttattat
     1561 aaacttctgc tgcctgcgcc cccgcagcaa acatcactgc cactgctaca gcagaaagtg
     1621 ccactgtcat ttttttcatg atttaatcct tatttaaact gaactattca tgcatttaga
     1681 tgtcatgaac aaaaattaaa atattttaat acaaattctt agttcaattt atattatgta
     1741 acaaaatgta aatacaagat cgatttcgca ctttctgaca agacagatta aaaataatta
     1801 acaactaaac aataaagagt gatttatagg ataattctca tctgactacc tgctctatcg
     1861 tgcaactgaa atcactgtac cgggcgttat ccgtaatact ccactcccgg cacaccaccc
     1921 tgaccgtccg gttatgtttc gggggcttcc acaaaaaagc ccggtaacca ccatgccatg
     1981 acagaaacgc ggacagtgcc tcccgctcag catccgttgt cacccgaaaa accacctgaa
     2041 aggtcttcag ttgcgcattc agtcctgccg gacgacgttg ctgataaccg tcgccaaact
     2101 tcaccgtcac caccgacggt ttctccgtca cctgcatccc ttcccgggga caccagtgaa
     2161 gggtcttaat ctcatccact cagcattcct ccgtcacgac gcatggataa catcaccgcc
     2221 tgtacccgct ggtcaatcag ttgcacaagg gtgcccgccg cttccggccc tatctgcccg
     2281 ttagcgccgt cattctgaat ggcaatgtgg tacaccgggg aatacaccag acccgcactg
     2341 ccgttcatac tgcccaccgc acgcacgccg agtgaaccat ccgctgcccg ggtcaggggc
     2401 atgatggctt caggtccggc ttcccccatc agccctgccc cttttgcaaa ggcaaagtac
     2461 gtgggcgtgt ccacaatact gttactgtat gcactcaggt ttgccgaggt ataaacgccg
     2521 ccttttgcat tggccaccgc gccacccagc cagttgccta tactgccgaa aaatcctcct
     2581 gcaccggaca tactgtttgc ggccatctta atgccgttga caatggccgc attcataaga
     2641 acttttgata tttcctgcag gattgatgca gcccagctgc gccattccac tttatttccg
     2701 ttcagcatct ccgtgatgtt attcaccatc cctgagatac cgtctgtcgc aagctgtgct
     2761 gcctgtgaag cgtaatctga tgcgctgtcc acccagttac ttagcccttc ctgcagccct
     2821 ttctgccagt cagcacgctg cgcatccgat tcggcataaa aagcctcctg gtctttaagg
     2881 cgttcactca gatactgcgc attctgcgcc agcgcctgtc tgtaaaaatc ctcactgata
     2941 tcccctgtct gatactgaga ctgcaggtcc gcatccttct ggcggtagcc gtcacgaatc
     3001 tgctgcagct cccgcatccg ttcacggacc cgctcaccct gcccgtaccc cagcagctca
     3061 gcgtcatttg atgccctttc gtctgcatta tcatttttca gagcctcctc ccgggcccgt
     3121 aactgctccc ggattttctt ctggtcaatc agggcagcat tgcgcagcaa ctcctgcttc
     3181 tgtatctccg tcagggtttt cagctctccc tgcgaggtct ggtacttcag cttcgccagt
     3241 tctgtattct tccctgccag tgccagttgt tccttctgct gcttcagcag acgggaaaaa
     3301 ctgtcttccg ctttttccgt ctcagatttt ccggcccggg atttgggttt attcgcctca
     3361 ttattgcgcc aggcttccat cgaattacgg atgtaacgct gcctcgcctc ctgatacgta
     3421 tcccccacca gaccaaggtc atccgccgca taccccagcc gggcacgctc tttttcttcc
     3481 cctttcagcc gggacagggc cagttgacgc tctgtgttat tcagggcgcc ctgctgttta
     3541 tcatccagag tggcctgtgg cagtcgcagc ggtacactcg ccagtccctg acgctgttgc
     3601 agcagttcat tacccagccc cagcaggcgg ttgaattccg tatgctgacc gttcatgatc
     3661 agcagtgact gatacgcttt attctgctct gccgcctgct gacgaattaa cacaacacgg
     3721 cgctcttcca gtccggcaag cacatcctga atggactgcg ctttttcctg catctgtgcc
     3781 agacgggact gctcaacagc aagctgctct gttgcctgag caagcccttc cgttacagtt
     3841 ttcaccgatg tcaggtggtt tatcatgaag ccgttaccgg tcgtccagcc cggattagac
     3901 agcacatact gatacccggc gattttttcc tgcagggatt tcacccggct ggcctgctca
     3961 tcaatcagcc ggttctgctc tgtcagcgcc tcccgtgttc gctcttcatt atctgaggct
     4021 tcaggcagag acattgatgg cgttttatgc gcgatttcat caatcgtcag tgcatactgg
     4081 cgcgcagact ccctggcctg ttcctgattc tggtacagcg tgtaccatgc ggcagctccc
     4141 agcatcacca gtccgggtac gcctccaacc agtcccagcg caccgctcat cagacgtgag
     4201 cccaccgccg ttgtactgtt cagcgcattc tgggcggcgg ttctggcagc aatatttctg
     4261 ttcaggcgtt cctgtgtgac cgccagacgg gcctctgccg caatctgcat ctcagtcccg
     4321 cgggctgccg ccacagcctg ctgagcccgg tacacggctg cccttgcccg cgccgtggca
     4381 atctgcgttc cccttaactg ggcctcagcc agtgccactt cattacgtgc agcggtcaca
     4441 agtcctgccg tggcagacat cgctccggaa gccagattac caaagtaccg ggccaccccg
     4501 acagcaacca gcacacccgc agctgttgcc acggtatcaa tatttccggc cacaccgttc
     4561 agtgcgccag agagcgtttt cgtcgctccg ctggcctcat tcgcgccgcc cacccaggcc
     4621 ataaaggcgt tttccacctt cgtgatcccg tcagacaccg tttccggcat ggctgcatat
     4681 tcatcacgca atatccccag ctggctgatt aacgcgggaa cgactttatc cgccgtcagt
     4741 tgaccatcgt ccgccatcgc cttcagatcc ttacgggcca cgcccatgcc tgcagccagt
     4801 gcgcgaatga tccggtcccc actttcattg accgaattaa attcctcgcc gcgcaacacc
     4861 ccctgtgcca gcgcctggct gaactgggtg atcaccgaac ccgcctctgc cgcactggca
     4921 ccggaaattt tcagccctgt cgaaatggcc tccgtcacct tcagcacatc atcagcactg
     4981 taaccatatt cacgcatcga ggcagccgaa cgggcaaaca gggccgcatt atccgaaaat
     5041 gcggtgcctg tccgctggct gatatccatc agcactttct gtgatgacga aaattcatcc
     5101 gatgactgcg acgcctgttt cagacgggca ttcacggagc tccattcatc cgccagcgaa
     5161 atcaggtgtc cggtggcaaa ggcaccagca aacgcaccgg tcattccgac agccgaagcg
     5221 cggatttccg tcaactggct gttcagctca gccagagccc ggcgctgctc cctggctgcc
     5281 gcagcagcct gacgtccgcc attctgcagg gtccggtaat attcactgcc catacgggac
     5341 gcccgctgga tctccgactg gaatgactgt gaatttgccg aaattttgat aatcagttca
     5401 cgtaacgtcg ccattcacct ttctccgggc gaaaaaaaac ctgccgcagc aggttttatt
     5461 catcagcaga ttattgatta agttttgggt accagcccgg gattcagact ttccggactc
     5521 agtgtataca taccgccatt gaatgggtcc acaagcaacc agccaacaat accaccaaag
     5581 acaaagttac caccaatgta ccatccatta gccgatgcct tgatcggcag cgttacgggc
     5641 tgataattct ctttactgaa tgtgatctga taactctttt tcccgaagta actgccgtca
     5701 gatttttcaa gtgttacatt ctgcggtgtt ttgcccgaag aaattgtttt ccctgactca
     5761 tctttaataa taaaatccgc accagacgga ttactgttta cctgtacagt ctgcgtttta
     5821 tctccgacaa tggttgcaca ccccgtcaga aaaaagctga cagagaaaat caacagcatg
     5881 aatttcttca taaaatggct ccgttcagtt aattttcccc gaaaatactg taataaagat
     5941 ctgcaaagtt aaaattaatt ttccgaagca aataacagag catcctccag agcggccaaa
     6001 ggatctgcct ccggcttatc ctcatcctcg ccccagcaga gcatggcgtc cttcagtgca
     6061 acattcatcc cctgtgcccc gaaaaccgct ttcacgatct gtgcattacg gatatccccg
     6121 cgctcatcac ccagcgggga taccctgtcg aactccatcc acatcatcgc ctcgctcgca
     6181 ctcaggctgt ggcgcagttc ggataaggtg cgccccagac ggagcgcaag tcgcatcaga
     6241 aagcgaattt ccgggcgggc tacttttttc tggccgactc tgcatcagcg atcagttcca
     6301 gtgcctgacg cagcaagcgg gcatgtaccg gaccatagac ggccagcacc tgctcacggt
     6361 cgtccggagt gaacacccgc tgcaggtccg tatcacacag gacatcgcag aacagcgtca
     6421 catccgcttc caggttacgg cgggttttcg ccaccaccga cagggtatcg tcatcctctc
     6481 catcaccatt gagcacgtcc tgccacagat accaggcctc tgccgaaggc tcccgcagca
     6541 ccacgctgac attaccccat tccggcacct tcaccgtttt atgacgaaac cctgacagtc
     6601 tggccagcgc cagcgttttc agatcctttt tcatgatgac ccatcccctt atccggcggc
     6661 tgcgctcact gtcactgtac attcaacaga cgtcacactc tgtgctttct ctgccgaatc
     6721 agtcaccacg caggtatatt tccccgcatc agcggactgc gcacctggct tactgaaggt
     6781 gtctgtcgtc tgcccgtcaa ccggctgacc atccttcttc caggcgtatt tatacggcgg
     6841 cgttcccccg ttggcactga ctgacattgt cagcagcgca ccggtattca cggtaagtgt
     6901 cttatccaga tttttcacaa acgccagcgg taccacaaag gacaccggtt tgcctttcag
     6961 acgcagtgag aacgttgcag ccaccacgcc gttggtaccg gatgaccagg tgtgctggcg
     7021 cacttccgcc aggaatttaa agcccttacc ggacggaaac tgcaccttaa acgcatacaa
     7081 cgcgtcattg tcataagcat cacgcagggc gttctgggcc tgattcagat aaaaattacc
     7141 cgacatggaa atctcggacg acgcccccag accattgata ttctcctgct ctgtggagca
     7201 gagcgtggtc acatcaatat cctgtttctg accggcggtg aactggactt ccttgatggt
     7261 gcagtccagg cgcagatatt ccgccttatc catagtttca gcagtcgccg gggcagatga
     7321 aatcatcacc tgcgtcagct gtgagcgttc atacaaagca gacattctgc ctcctgataa
     7381 taaaaaaccc gcacgcggcg gggtatgggt tttgtagaaa aaagaaaaag tcacaccgtg
     7441 acctgaaact ccagggttgc acggtaacag cggttttccg gaatatagtc ctgcatttca
     7501 ctgacggatc ccggggccag cagcattatg gcttcacggg cgtcctgacg tatctgacgc
     7561 gcctgcgtca cagtcccggc ataaacgtct atctgcaccg ccactgagga ctccgcctgc
     7621 ccgcccatca cgtccgcaga caccgatgaa atcaggctga aaaccaccca cggaagcgcc
     7681 accgacggcc tgccatccag cagggggacc acatacgggt acacctgccc gccggcaaga
     7741 tgcgccagat gaggatacaa atccgcctcc gtcatcgtct cagtacctca tcaatggccc
     7801 ggttcatccg cgcaatcgcc acctgcgctg cctgttcact gcgcacatca aacgccgggc
     7861 gcacaaacgg gtgcggtggc atattcacgg tccccatttc cacaaaccgc cagtagaaag
     7921 cattccgcgg gttatctgcc ttcatggtgt tatcgctgtt accggtgtcc ggattaacac
     7981 cacggatatg gacaccggat tctatcccgc catcgcggga gcgccgggaa aggaccacca
     8041 cattgcggcg cagttttccc ctgcgcaccg gtgcccgtga caccacttct tctttcagca
     8101 cattcgcacc cgcacgggtt gcctcacgca gcacccggtt attttccgca ccactcagaa
     8161 gctgcaaatc gcggctgatg tcctccagcc ccgaaaaatc cagcagggtt tcgatcattt
     8221 ttcccctccc agccgacaga gaatttccag acgcccgccg gtcgcatccg gcacgggcag
     8281 cccgacaacg ttcaggatcc ggtcacgcca tggaccactc agcacatgaa gtcgtgacgc
     8341 tgccgtgatt tcccgaccgg actgaccgcg cacccagatg cggatttccg cctgcgccat
     8401 ttccgcaccg gactgcatcc gctcccggct gctcctgcca cggatatccg catgaatttt
     8461 cccgcatgac acccattctt ccgtcatttc tccggcagca ttacgggtta acaccgggtt
     8521 cagaacactt atcatctgtg tcagacgacc tgcagatatt gccattcccc ctcctcataa
     8581 caccgtcgga caacgcaaat cgtaaatcag cacggaaaca gaaaacggca gctccccctg
     8641 aagcagttct tcccgctccg caagatccgg attccggtac agcatcccgg tcagtcgcat
     8701 ggcagccccc ttcatccggg ttaatgcctc gcccgggatc agttcaccgt cctcacgaat
     8761 caccttatcc cggctgccct gaatgtaggc cagcagcacg gcggtagcct gacgaacctt
     8821 gtccatcagc atgtcatcat ccgcgtcatg gtcgacacgc agatgtgcct tgatctcttc
     8881 cagtgtcagt aatgccgtca ttttccacct cctgcatccc gcccacgttt tgcagccagg
     8941 gtccaggctg atgaatgagc ttctccgggt ttatcaccgg tcatactgtt gcagtgccac
     9001 agcgagcccc cccatgtcac cgtatcgccg gggtggtagg tttcaccggc tctgaacaca
     9061 ccgcggtaga gcatcaccgg cagggaaaat gttttttccg tacgctggcc actgctctgc
     9121 cggaccacca cagagaacaa ccgttcaccc gtcatgctga cgtcaatatc cgccaccccg
     9181 tcaaccaggc attcccatcc ccgcatcccg tgcgtttttt catacgcccg ccagagtccg
     9241 ccctggtgtg tggcatacgt gccccgggga aaggattttt gatcgtcaat ggcagggagt
     9301 atttccagtg ccgtggcatc acgcccgtcc tgcggagccg gcagggcact caccgcatcc
     9361 agaaccgcct tctgcagaac atcgggatca tagtcacgac catcacgcgg aacaggaata
     9421 tggcttaccg cctccttcac catctgttca agcatcggac gcacatcatc cggggtgata
     9481 cttttaccgt ccgccggctg cggaatattt gcgaccgcat cattcaccgc cttctgcaga
     9541 acatcgggat catagtcacg accatctcgc ggaacaggga tatggcttac cgcctccttc
     9601 accatctgtt caagcatcgg acgcacatca tccggggtga gacttttacc gtccgccggc
     9661 tgcggaatat ttgcgaccgc gtcattcacc gccttctgca gtacttccgg atcgtagtca
     9721 cgaccgtcac gcggaacagg gatatggctt accgcctcct tcaccatctt ctcaagcatc
     9781 ggacgcacat catcgcccgt cacgcacttc tgtaatacca ctgacaggga cgccagtttt
     9841 tcttcaaacg tttgtgcctg cgcggctatt ttcccctcaa atgtgcgctg taaatctgcc
     9901 agcactgcgg aaaattcttc acccagcgca cggataatgg acagttcacg ttcattcatt
     9961 ttttcagaat ccccctgaac atcgccttca ccgcatcatg ctctgtttca gtgattgcct
    10021 tattaccgtc agaggcagcg accgcctgag gcacggaagc tgttttaccg gacgacgcga
    10081 acggatcttc acgggcatca cggcgggaca acgcttccag actgtagttc tgctgctgaa
    10141 gatacagtgc atcaccgccg gccaggggcg gcaggttctc ccgtttacgg gcctcattgg
    10201 gcgtgagaag cgtatttttc accgcatccc ccagcgtttt catgcgccgc tcactgtcca
    10261 ttctcagcag cgtggtgaca tcaaattccg tactctcgtt ttcccccgtt tccagcgcct
    10321 catccagtaa cagctcaatg gactcaatca gtgtctgcag gcactgggaa taatactgct
    10381 gctccagcgc ctccacgttg tcactggaag gcggttgtcc cacgccaatc ttgtaggccg
    10441 ggacacggaa caccgaacag acaatttcag cggtcatctt cagttgttcc accgtctgtg
    10501 catccaccgg tgaaaacgtc gtggggttgt attttgcccc gttgctcaga atggccgttt
    10561 tccccgcatt ttcgcctgta tacccgctgt cccagttgct cttcagtttt ttcgcatttt
    10621 cttccgtaat actgccgggg atctcaatca ccccggacgg cctgccgcca tttctgaaaa
    10681 aagacgtcga attttcctga atatgatgcc cctgcgtggc cgccagcccg gcggcataca
    10741 ccggcggcaa ccctataagc ggatgaaaaa aacagttaaa ccggtcgtgg atcacttccc
    10801 gggcaggcac cgtcaccgcc tcagggatcc cgcagttccg gtccggcgtg atgcggtaga
    10861 acacctcgcc gtcatccgcc accagaggtt caacccggtt ccagtccaga atacgcagtt
    10921 ctttgatctg cccccgggca ttacggattt tcagcaccac cgtattgccg tgacgcagtt
    10981 tggcgttcag ccacagttca aaaaactgga tgcggttctg ctgggcgttg ggacgacgac
    11041 agaggcgggc aatatccccc cggcgcgttt ccctgcgtat cccctgcgca tccgtctgca
    11101 tcaggcgcag ccgcattttg gcgatatcct gggatatcag cgaaatacat gcaaacaccg
    11161 catgaaagga gaggacggct tcaggatcgg ctttcacgcc ctgctgccag gcaccggaaa
    11221 agggctcagc caccgcctga aacaggctgg tccagcccgc ctctcttacg tcacgtcctg
    11281 atttctggtt ttttcgggtt cgccgtaaaa ggttccacat tcgccatgct ccgcatcacg
    11341 tttctttttc tgacctgccg gacgttgcac cgtgatgtac tccgcctttc ccaggcgaac
    11401 cagcacctcc gcacacggct gtgccacatc acggatatcc ccggcccggg catcatgcgt
    11461 gccctgcaga tatcggatct ttgccataac ctgttacggg aggcgcacgc ctcccgtcct
    11521 ccttatcaga ctcagccgcc ggacgcactg ccgtagttca ctccggtgat caccgccacc
    11581 gccgcggtac ggcgacgacg ccagttgatc cagcgctccg cacggatggc cacgctgcct
    11641 gtctggaaca tggaaaccag ctccaccggc gacggcgtgg tactgtcgcc ggtcggctca
    11701 gactgcattt ccagtgatgc ctcgcgggac atatccactg ccacgccgcc gtcatccgcc
    11761 agataaatat ccggggcatt caccagcacc agctggtcac ccacgtactg ggagacaatc
    11821 accggcagcc cctggaagga gccacccagc agggtcatgt ccgggtattc tttctgaccc
    11881 agcgcatttt tacgcatgga cagtgccagg gcattggtgc tggacatcag ccagaccgca
    11941 ccggtgggtt gcaggtttgc tgccacaaac tgtccaaacg cagcctctgc atccgcatcc
    12001 gggttaccgc ttgatgccgt gcccttcaca tcatgggtga tggacgccgg ggagacatct
    12061 gccaccgc
//
LOCUS       DAZFKF010000134        11556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFKF010000134 DAZFKF010000000
VERSION     DAZFKF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.136"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            203..973
                     /gene="yjjP"
                     /locus_tag="ACHKA1_004561"
     CDS             203..973
                     /gene="yjjP"
                     /locus_tag="ACHKA1_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO5682117.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            964..1437
                     /gene="yjjB"
                     /locus_tag="ACHKA1_004562"
     CDS             964..1437
                     /gene="yjjB"
                     /locus_tag="ACHKA1_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO5682118.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1544..2083
                     /gene="dnaT"
                     /locus_tag="ACHKA1_004563"
     CDS             1544..2083
                     /gene="dnaT"
                     /locus_tag="ACHKA1_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO5682119.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKTEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVDLDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2086..2823
                     /gene="dnaC"
                     /locus_tag="ACHKA1_004564"
     CDS             2086..2823
                     /gene="dnaC"
                     /locus_tag="ACHKA1_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO5682120.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2872..3366
                     /gene="yjjA"
                     /locus_tag="ACHKA1_004565"
     CDS             2872..3366
                     /gene="yjjA"
                     /locus_tag="ACHKA1_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO5682121.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3620..5911
                     /gene="opgB"
                     /locus_tag="ACHKA1_004566"
     CDS             3620..5911
                     /gene="opgB"
                     /locus_tag="ACHKA1_004566"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO5682122.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQTVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6049..7071)
                     /gene="lgoD"
                     /locus_tag="ACHKA1_004567"
     CDS             complement(6049..7071)
                     /gene="lgoD"
                     /locus_tag="ACHKA1_004567"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO5682123.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATLEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7210..8124
                     /gene="lgoR"
                     /locus_tag="ACHKA1_004568"
     CDS             7210..8124
                     /gene="lgoR"
                     /locus_tag="ACHKA1_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO5682124.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVMARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8339..9700
                     /gene="lgoT"
                     /locus_tag="ACHKA1_004569"
     CDS             8339..9700
                     /gene="lgoT"
                     /locus_tag="ACHKA1_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5682125.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9749..11413)
                     /gene="tsr"
                     /locus_tag="ACHKA1_004570"
     CDS             complement(9749..11413)
                     /gene="tsr"
                     /locus_tag="ACHKA1_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO5682126.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
BASE COUNT         2805 a         3050 c         2808 g         2893 t
ORIGIN      
        1 attcattata gtgataatat tcatgtgata atttgtttgc ggcgagagat aattcgcttt
       61 ttatcaccga gcttctcccg ataaccagga ttaatcagcg caattttgat cttaacctac
      121 cgcaaaacaa ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca
      181 cgtctatgac aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cacggctttg
      241 tatccagtgt ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct
      301 ttcctcacga ctgggtcggg cgctgggaat ggacagcgta gaaagttcta tctcttcgaa
      361 cgccatagtg ctgacaacta ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca
      421 cgatcgcggc attaatatgc atgtggtgac tgaagtccag cacattgtga ttcttgcgga
      481 gcatcatctg ctggattaca aaggcgtaga gaaacgattt agccaaattc agccattacg
      541 ttacccaaga tggctggtag ccttaatggt tggcctttct tgcgcctgtt tctgtaaact
      601 caataacggt ggctgggatg gtgccgtcat caccttcttt gccagtacgg ccgcgatgta
      661 tatccgccag ctgctggcac aacgtcatct tcatccacaa atcaactttt gcctcaccgc
      721 tttcgccgcc accaccattt ccggattgct tttgcaactc cccactttca gcaatacccc
      781 caccattgcg atggccgcca gcgttctgct gctggttccg ggctttccgt tgattaatgc
      841 cgtcgccgat atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag
      901 tctgctgaca ctggctacct gcgtcggcgt agtgatggca ctgacgattt gggggctacg
      961 cggatgggtg tgatcgaatt tctgttagcg ttggcgcagg atatgatcct cgccgccatt
     1021 cctgcggtcg gctttgcgat ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg
     1081 ctgcttggct cgataggtca tggttcccga atgatcttga tgaccagcgg gttgaatatt
     1141 gagtggtcaa cctttatggc ttcgatgctg gtcggtacca ttggtattca atggtcgcgc
     1201 tggtatctgg cgcatccgaa agtgtttacc gtggcggccg ttatccctat gttcccgggc
     1261 atatcggctt ataccgcaat gatttcggcg gtaaaaatca gccagttagg ttacagcgaa
     1321 ccgttgatga ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcgtta
     1381 tccatcggtc tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat
     1441 tgcctcgctt aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa
     1501 gttatctccc ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt
     1561 gaccccggac gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa
     1621 aactgaaggc ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac
     1681 gcctgcacgc ctggctgaac tgctggcgct ggaagaaaag ctggcgcgtc cgggaagcga
     1741 tgtcgatctg gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtacccat
     1801 ggggaaattc gccatgtatc cggactggca acccgatgcc gactttatcc gcctggcggc
     1861 gctatggggc gtggcgctaa gagagccggt gaccaccgaa gaactggcct cattcattgc
     1921 ctactggcag gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg
     1981 cagcctgcaa atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt
     2041 cagtgaacct gacagccaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc
     2101 gacctgatgc aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg
     2161 ggtgaagagc tactggcctg gcagaaagaa caaggggcga tccgctccgc cgctctcgaa
     2221 cgtgaaaatc gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcgtccactg
     2281 catcagaact gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta
     2341 agcaaagcgc gccagtatgt cgaagagttt gacggcaaca tcgccagctt catcttttct
     2401 ggtaagccag gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta
     2461 cgcggtaaat ccgtattgat catcaccgtg gccgatatta tgtcagcgat gaaagatacc
     2521 ttcaggaata gcggtaccag cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg
     2581 ctggtgatcg atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac
     2641 cagatcgtcg atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat
     2701 atggaagaga tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac
     2761 agtttgtggg tgatcttcaa ctgggatagc taccgtagcc gggtaacagg taaagagtat
     2821 taatccccgt ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa
     2881 actgttaaac atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg
     2941 catgctgcgt cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac
     3001 agcacgacac aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcgga
     3061 aaccaggcct taagcgcaga taacatgaac aatgccgcag gcattctgca atactgcgcg
     3121 aagcaaaagc tggcgtcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag
     3181 ctgggcctga acagtgaaga gcaaaaagaa gacaccaact atctggacgg cattcagggt
     3241 ttgctgaaaa ccaaagatgg tcagcaactc aatctggata acatcggaac aactccgctg
     3301 gcagaaaagg tgaaaaccaa agcctgcgat ctggtgttaa aacaggggct gaacttcatt
     3361 tcctgaaccg cgaaacgccg cgttttagct gttacccggc tgacgcggct ctctcctgcc
     3421 cttttccctg ctcctggacg gttttacccc tgttacacgg gaagtgaccg taatcaaaaa
     3481 agtctgttta taaactaatt cctaacaaca tcgcattttt ttgacactag aattgcagca
     3541 atatggcgac gccattacat tgtgcttctc aaaaagctta aacgttggct aaacggcccg
     3601 gtttagtgag gatcgcccgt tgtcagaact actctctttc gccctttttc tcgcctctgt
     3661 gctgatttac gcatggaaag cgggacgtaa cacctggtgg tttgcagcca cgttaacggt
     3721 gctggggcta tttgtcgttt taaatatcac cctgtttgcc agcgactatt ttactggcga
     3781 tggtattaac gacgcggttc tctatacctt aaccaacagc ctgaccggtg ctggcgtcag
     3841 caaatacatc ctgccgggta tcggcattgt gctggggctg acggcggtgt tcggtgcgct
     3901 gggctggatc ctgcgccgtc gtcgtcatca tccgcaccat tttggttaca gcctgctggc
     3961 actcttactg gcgctgggtt cggtggacgc cagcccggca tttcgtcaga taacggaact
     4021 ggtgaaatcc cagtcacgcg atggcgaccc ggactttgcg gcttattata aagagccgtc
     4081 gaaaactatc cctgacccga aactcaacct ggtttatatc tacggcgaaa gtctcgagcg
     4141 gacctatttt gataacgaag ctttcccgga tctcacgcct gaactgggcg cgttgaaaaa
     4201 tgaaggcctg gatttcagcc acacgcagca gctgccagga acggattaca cgattgcggg
     4261 catggtggct tctcagtgcg gcataccgct gtttgccccc tttgaaggca acgcctccgc
     4321 ttctgtttcc agcttcttcc cgcagaacat ctgtctgggc gatatcctga aaaactcggg
     4381 ttatcagaac tatttcgtgc agggcgcgaa tctgcgtttt gccggtaaag atgtgttcct
     4441 gaagtcgcat ggcttcgacc acttatacgg ctcagaagag ctgaaaagcg tggtagccga
     4501 cccgcactat cgcaacgact ggggattcta cgacgatacc gttctcgatg aagcgtggaa
     4561 aaagtttgaa gagctttccc gctcaggtca gcgattctca ctgtttaccc tgacagtcga
     4621 tacccatcac ccggatggtt ttatctcgcg cacctgtaac cgcaaaaaat atgattttga
     4681 cggtaagccg aatcagtcat tcagcgcggt aagttgcagc caggagaata tcgcgacgtt
     4741 tatcaacaaa atcaaagcgt caccgtggtt taaagatact gtcatcgtcg tctcttctga
     4801 ccatttagcg atgaacaaca cggcgtggaa atacctcaat aagcaggatc gcaataacct
     4861 gttttttgtc attcgtggcg acaagccgca gcaagagacg ctggcagtga agcgtaacac
     4921 gatggataac ggcgcgacgg tactggacat tctcggtggc gataactatc tcggacttgg
     4981 tcgtagcagt ttatccgggc agtcgatgtc ggaaatcttc ctcaatatca aagagaaaac
     5041 attggcgtgg aagccagata tcatccgcct gtggaaattc cctaaagaga tgaaagagtt
     5101 caccatcgac cagcagaaaa acatgattgc cttctcgggt agccatttcc gtttgccgct
     5161 gctgttgcgg gtttcagaca aacgcgtgga accgctgccg gaaagcgaat actcagcacc
     5221 gctgcgtttc cagctggccg atttcgctcc acgcgacaat ttcgtctggg ttgaccgttg
     5281 ctacaagatg gcacaactct gggctccgga actggcactt tccaccgact ggtgtgtctc
     5341 gcaggggcag cttggcggtc agcaaactgt tcagcatgtc gacaaaacaa catggaagag
     5401 caagacggca tttaaagata cggtcatcga catggcgcgt tacaaaggca atgtcgatac
     5461 gctgaagatt gttgataacg atattcgcta caaagccgac agtttcatct ttaacgtcgc
     5521 cggtgcgcca gaagaggtga aacagtttag cgggatttcc cgtccggagt cgtggggccg
     5581 ctggtccaac gcgcagctgg gcgatgaagt aaaaatcgag tacaagcatc cgctgccgaa
     5641 gaaatttgac ctggtgatta ccgccaaagc atacggcaat aacgccagcc gtcctattcc
     5701 ggtacgcgta ggcaatgaag aacaaactct tgtgctgggc aatgaagtga ccaccaccac
     5761 actgcatttc gataacccaa ccgatgccga cacactggta attgtgccgc cggaacctgt
     5821 ctcaaccaac gaagggaata tcctcggaca ctcgccgcgt aagctcggga tcggcatggt
     5881 ggaaattaaa gtggtagaac gtgaagggta attcctgact gatgtgtagg tcgcataagg
     5941 tgcgacctac acaccttctc cggcttacaa cacattatta agaaacaaaa aaaacagcgc
     6001 ccaacacttc cccggacgct gcatggcaac tttaataatc tcgccttatc agaaagtaat
     6061 tacgccttta attaactcac gattgttaat cacatcgcgc tcgtaggttt ctgccagcgt
     6121 ggcgaacgga tagcgatggg ttaacatcat gtcagcagtg atttttcctt ccgccatcag
     6181 tcgaccgact ttagcaaaat cttccagcgt ggcgttgcgg ctacccatca tcgtcgtttc
     6241 ttttttatgg aattcaggat cggagaactg caactctcct ttaaacaggc cgacaaatac
     6301 caccgtgccg ccgtgacgaa tcagattcac ggtgttattc atcgcatgtt gattgcctgt
     6361 cgcgtcgatc actttctgcg ccagcgaacc accaaactgc gcccgcagct gcgcgtcaaa
     6421 atcttcggct gacgggtcca gcacgggcaa ctccagacgc gttgccacat gttcacggcg
     6481 cgccggactg gtatccgcca ccaccacctg tgcgccatcg gctttagcga ttgccgccgc
     6541 gcccagaccg attggccccg ccccaaccac cagcacctgc tcgccgggag caattgctgc
     6601 gcgacgcacc gcatgagcgc taatagcgaa aggttcaatc aatgcggccg cctgcgggtc
     6661 aataccgtct gcgggcaaaa tgttcgccac cggcacgctc aaatactcac taaaaccgcc
     6721 atcctgatgc acgccaatga ctgaaatttt ttcacagcaa ttggtacgcc cgcttttaca
     6781 cgccgggcat tgctgacagg caacataagg gatcacagca acttgctgac catttttaag
     6841 atcagcaata tttttaccca gcccaacaat ctccccacat atttcatggc ctaaaacccg
     6901 tggataacta aaaaatggtt gatttccacc ccaggcatga atatcggtac cgcaaatccc
     6961 gacagactta atttttatta atgcttcatt gtcacccgga ataggtatct cgcgttgttt
     7021 ccagactaat tctttcggct gctggcaaat taaaacattc atcgtagaca tgattcggct
     7081 ctccattttt tgttgcctta gatatcaatg aaaatgaaaa atggcagcgg aacggaaaat
     7141 ctgttttgtg aaaacacacg cataaaaatg ttttaaatcg ggttttaatg gactcacaag
     7201 gaggaaaata tgagtcgttc acaaaattta cgccacaatg tgattaacca ggtcattgat
     7261 gatatggcgc ggggtcatat cccttcccca cttccatcgc aaagtgcgct ggcggaaatg
     7321 tacaacatca gccgcactac agtgcgtcat attctcagcc acttacgcga atgcggcgtc
     7381 ttaacgcagg ttggaaacga ctatgttatg gcgcgtaaac ctgaccatga tgacggtttc
     7441 gcctgtacta ccgcttcaat gagtgaacaa aacaaagtgt ttgagcaggc ttttttcacg
     7501 atgataaacc agcgtcagtt acgccctggg gaaacttttt ctgaactgca actggctcgg
     7561 gccgcgggtg tcagcccggt ggttgtaaga gaatatcttt taaaattcgg ccgttataat
     7621 cttattcaaa gcgaaaagcg cggccagtgg agcatgaagc aattcgatca atcctacgcc
     7681 gagcagctgt ttgagctacg cgaaatgctc gaaacacatt cacttcagca ctttctcaac
     7741 ctgcctgatc atgatccccg ctggctgcag gcgaaaacca tgctcgaacg tcatcggcta
     7801 ctgcgtgaca acatcggcaa tagctttcgc atgttctcgc aacttgacag agattttcac
     7861 tccttactgc tttcagctgc cgacaatatc ttttttgatc aatcgcttga gatcatctca
     7921 gtcatctttc attttcacta tcaatgggac gaaagcgatc ttaaacagcg caatatcatt
     7981 gccgtcgatg agcatatgac cattctcagc gctctgattt gccgcagtga ccttgatgcc
     8041 acccttgcgc tccgtaacca tttgaattca gctaaacaat caatgattcg atccattaat
     8101 gagaacacgc gttatgctca ttaagtacgg attaaaaaca gcaaactatt agcgggtgca
     8161 cggttttatc aaaagataaa taccgggaaa ttcccgctta cctatgctca caatcagtcg
     8221 actaaaaatg tttttgtgag cggtagtaaa gtcctaaaac tttaacctgc cgttaatgtc
     8281 aaccttgcgt aatgcacaat tacgtaaaca attaaaatta taaatacagg agtccggcgt
     8341 ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga
     8401 tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac
     8461 caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct
     8521 gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc
     8581 tttgctctcc gtgttttcac tcgcttacgg cattgcgcaa cttccttgcg gcccactatt
     8641 ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca
     8701 ggccatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta tcggtatggg
     8761 gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact ggttcaacat
     8821 caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgccgt
     8881 aagcccaccg attctggcgg cgatgatgct ggtgatgggc tggcgcggga tgtttattac
     8941 cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc gcaaccgcga
     9001 gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg
     9061 ccgagatccg ctcagttttg ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg
     9121 aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cttggcttcc
     9181 tggttacctg caaacagcct ataacctgga tttaaaaagt acagggttga tggcggctat
     9241 ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt
     9301 caaaggggga atggctccga ttaaaagccg taagatctgc attattgccg ggatgttctg
     9361 ttctgccgcc tttacgctga tagtaccgca agcgacaaca tccatgacag cggttctgct
     9421 gattggtatg gcactgttct gtattcactt tgccggaaca tcctgctggg gcttgatcca
     9481 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
     9541 catctgcgcc tctttcgcgc cgatcattac tggttttatt gttgatacaa cccactcatt
     9601 ccgtctggca ctaatcattt gcggttgcgt caccgcagcg ggagcactgg cgtacatctt
     9661 cctggttcgt cagccgatca acgacccgcg taaagattaa actccctatg cccgataaca
     9721 ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctcc ccgctatctg
     9781 ccactgccat tttacgcggc gtagctggcg tcacggtttt taccacagcc gatgtttcac
     9841 gctgctgttg ctgctgttgc tgaatccgga acactgccac agcttcggtc aggcgactgg
     9901 cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg gcgttctgtt
     9961 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
    10021 catcagaagc agaagctatt tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa
    10081 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
    10141 ccgagtcttc aatcaggctt ttaatttcac gagccgcctg ggcgctacgc tgggccagat
    10201 tacgcacttc tcccgcaacc accgcaaaac cgcgcccttg ctcacccgca cgcgcagcct
    10261 caaccgccgc gttcaaagcc agaatattgg tctggaaagc aatgccgtca attacgctga
    10321 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10381 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taacgccagg tggctggcct
    10441 ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg
    10501 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcgtta ttaccggtgg
    10561 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    10621 gcatcagctc tccctgcata tggcgcaaac tttctgccag ttgccccatc tcattagagc
    10681 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    10741 gacgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    10801 ccagtacgac gatcatcacg cccaccagaa tccacatcgc ctggctgtag gaggcattgt
    10861 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    10921 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    10981 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    11041 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatctgccc
    11101 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    11161 aaccgctacc aatattattc tgatccatca tgtagcggat acccgcacgg ttgagggtgt
    11221 tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg
    11281 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaagaac agaccgcctg
    11341 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11401 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctgaaatga
    11461 gaaaacttta taggtagcga cttgaaactg gttaatttta cctcactttt ttaatttttg
    11521 ttcaaaaata caataaccac ttattgatac attttc
//
LOCUS       DAZFKF010000135        11392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFKF010000135 DAZFKF010000000
VERSION     DAZFKF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.137"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..116)
                     /locus_tag="ACHKA1_004571"
     CDS             complement(<1..116)
                     /locus_tag="ACHKA1_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5682127.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKCRAMDAPGRRVCA"
     gene            complement(126..344)
                     /locus_tag="ACHKA1_004572"
     CDS             complement(126..344)
                     /locus_tag="ACHKA1_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682128.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            complement(328..678)
                     /locus_tag="ACHKA1_004573"
     CDS             complement(328..678)
                     /locus_tag="ACHKA1_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682129.1"
                     /translation="MAALGVMSVLAAVVWGMHQKTQRLEDNNHVLVRERDEARLVLAN
                     QQRTLQLISQISEAATNEKQQNIQHSEGQQSVVRRSLAAVPAASAPVPDDVADRVRRA
                     VCEIRACEAGADPR"
     gene            complement(791..1291)
                     /locus_tag="ACHKA1_004574"
     CDS             complement(791..1291)
                     /locus_tag="ACHKA1_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5682130.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTITTHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            complement(1363..1785)
                     /locus_tag="ACHKA1_004575"
     CDS             complement(1363..1785)
                     /locus_tag="ACHKA1_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO5682131.1"
                     /translation="MAETQMSMFGDSEQLHALIDRLDDIPDDELKKNWPGTLRDLVDV
                     ISAELHRQGIEPAQAGMLARKVAAAQAGYMGGRGYYLPVGESLFTELRNNEIFSRWSQ
                     GEKIETLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            complement(1891..2127)
                     /locus_tag="ACHKA1_004576"
     CDS             complement(1891..2127)
                     /locus_tag="ACHKA1_004576"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682132.1"
                     /translation="MDIFLYSGGTLSGRLKLFVFALEQAWCALRVGIIGFHVDETTIT
                     RNMHKLFDKKNSFRVCIFKNKNDLYHIVTPENDQ"
     gene            2126..2548
                     /locus_tag="ACHKA1_004577"
     CDS             2126..2548
                     /locus_tag="ACHKA1_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682133.1"
                     /translation="MKQPPSLLLSCPPELAHELNDLAKEYKLTNHNIEAATLSAAGSD
                     IVLIVLAAASCDGFWQALASMFQTWLHRKPTKQTPPEKKVIRSVIVRSWEHYQETTKQ
                     TEFYNFDADELAKLIALYEKPDETIGCIIPSETEIQEK"
     gene            complement(2575..3084)
                     /locus_tag="ACHKA1_004578"
     CDS             complement(2575..3084)
                     /locus_tag="ACHKA1_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO5682134.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSALLAATGKASCRDMSPDEL
                     SRVLDVFKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPELASDVLERLKRWHRRKMLAALDMSERAVMSYERVC
                     RLYEKSFPC"
     gene            complement(3081..3392)
                     /locus_tag="ACHKA1_004579"
     CDS             complement(3081..3392)
                     /locus_tag="ACHKA1_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682135.1"
                     /translation="MDEQITDNISLLFWCLYITSFFGAFVITRWLCRKIISLFDKRYL
                     LELAADALIQQAIVLYSGEFFCRITTRDGWHIMIIPPTHHIRWNEPEKAFHVRKKVNT
                     V"
     gene            complement(3385..3717)
                     /locus_tag="ACHKA1_004580"
     CDS             complement(3385..3717)
                     /locus_tag="ACHKA1_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682136.1"
                     /translation="MITTLFVESDEPLVCAAGMPVCGGTLTGVYFGDLRGYPWHSLND
                     AFPPDMEAVVLIVQYGHRQELRIGHIGYEGFFVDEETGACLEDEDGQVTHWCHISALP
                     ELREIHNG"
     gene            complement(3714..4265)
                     /locus_tag="ACHKA1_004581"
     CDS             complement(3714..4265)
                     /locus_tag="ACHKA1_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFO5682137.1"
                     /translation="MMILQPMGRKGQAPAHVRAWTPEEDALLIALYPSTPVKNIAVRV
                     KRSFWGVHNRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVHLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHRRTAGDAVLRELTP"
     gene            complement(4269..4784)
                     /locus_tag="ACHKA1_004582"
     CDS             complement(4269..4784)
                     /locus_tag="ACHKA1_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682138.1"
                     /translation="MFFKTSNPAALAAWQKYQQDCQTVKDEAKRLEAVLNVACRSVFE
                     FSISGFCFKGLRFMDDKYPFHRDLWRKPTASNGWSCTPRTSRIPKTLRVASDELNILW
                     REYSPVTYARTDALLFWLGIDFSAIMFGPVKWFCVDDVIYLECGVKPANQKMTEILSD
                     EFYAAEKRVRG"
     gene            complement(4784..5317)
                     /locus_tag="ACHKA1_004583"
     CDS             complement(4784..5317)
                     /locus_tag="ACHKA1_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682139.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCGILAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITEHVLRY
                     LIVDRTAAPAAISAEVE"
     gene            complement(5321..5863)
                     /locus_tag="ACHKA1_004584"
     CDS             complement(5321..5863)
                     /locus_tag="ACHKA1_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682140.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGSLPAGWRKRDDVAFIGDGPCFIARPDESCPEGPAIAAMIET
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNTDSWWTPSLSRDALCVVFKVGAYGREIKG
                     GIPEECQEIKHSEYVALTEE"
     gene            complement(5961..6491)
                     /locus_tag="ACHKA1_004585"
     CDS             complement(5961..6491)
                     /locus_tag="ACHKA1_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO5682141.1"
                     /translation="MVKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIHDWCKTNRNELTNGGETKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKQAVLAEPDAVKGVAGIKVNKGA
                     ESFHVEPFEQDAGLNK"
     gene            complement(6502..6795)
                     /locus_tag="ACHKA1_004586"
     CDS             complement(6502..6795)
                     /locus_tag="ACHKA1_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682142.1"
                     /translation="MSKVRVIFEFEHVSHDEKLAGNDCVEVHEKIGVDVKTERDTENS
                     PTSLCDVYASILQYHSPAIIQLLSAEFQASVQAFGADAIIKRHRVHKASGALQ"
     gene            complement(6800..7075)
                     /locus_tag="ACHKA1_004587"
     CDS             complement(6800..7075)
                     /locus_tag="ACHKA1_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682143.1"
                     /translation="MSKVVRIIFEYRENVIRKNADGSTHMGVSLDIRSTGIAKQKDGS
                     PAMIFGVVMLAESRNFAELVAMKASAFMKDRGMASGVINGNEFNQQG"
     gene            complement(7150..7380)
                     /locus_tag="ACHKA1_004588"
     CDS             complement(7150..7380)
                     /locus_tag="ACHKA1_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682144.1"
                     /translation="MNLFDSLNNARRLTELAGAVLERSKRYPQRFALKTTPPVNKVQG
                     GGKEIEITVKTNGLRRRVKATRISGCTVYWEV"
     gene            complement(7367..7786)
                     /locus_tag="ACHKA1_004589"
     CDS             complement(7367..7786)
                     /locus_tag="ACHKA1_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682145.1"
                     /translation="MNTQIQQEINVLHSFRAFNNNDCEVRDYIFELMNGDGVCFLFID
                     LYLWYADQQCESVRQRFYVGYFNHHVWESLRFGFLIQGDYRQGGRHGYSILRAIMRGE
                     TRKIPGMGEVDRSLLLELAASRMNPLLIQPEFTYEFV"
     gene            complement(7799..8020)
                     /locus_tag="ACHKA1_004590"
     CDS             complement(7799..8020)
                     /locus_tag="ACHKA1_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682146.1"
                     /translation="MAAFNIPDIYGRFYLVNVDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATDVYSGICRSVGAKQVR"
     gene            complement(8023..8955)
                     /locus_tag="ACHKA1_004591"
     CDS             complement(8023..8955)
                     /locus_tag="ACHKA1_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5682147.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELVVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            complement(9031..11118)
                     /locus_tag="ACHKA1_004592"
     CDS             complement(9031..11118)
                     /locus_tag="ACHKA1_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO5682148.1"
                     /translation="MFASVKELVGLPGMPGTEQGVRYSIRKYATSETSRRRQEGTKGF
                     EYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQL
                     MVERLNALTENQRQVADARIAIVSEVMKVAQQPGFSCAKAIRFIVDNLARSQLDERIV
                     AMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWLP
                     EFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVKQ
                     KGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVID
                     GGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGIL
                     PRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLRS
                     AFTALQQDKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPMKPNGKHYSP
                     AEFRKKRLAEEDTEIEWLSDVELRDMFRPMVKRPVRRCEIRWLNNIYYAPELRDEHGR
                     KVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRAE
                     EKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVAVQQPAVDDEPDEEYERNFQRGLQ
                     LLEAKLKENDPLA"
     gene            complement(11121..11351)
                     /locus_tag="ACHKA1_004593"
     CDS             complement(11121..11351)
                     /locus_tag="ACHKA1_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5682149.1"
                     /translation="MARNEVKPDWHPQRILGEVRAREGSMRALARKAGVGQNNLYNAV
                     YRHVPKYEGIIAECLGVPPEEIWPSRYIRKVA"
BASE COUNT         2712 a         3181 c         2634 g         2865 t
ORIGIN      
        1 gcgcagacac gtctgcccgg agcatccatt gcccggcact tacgtgtcag ccggttacaa
       61 agggcgcgtt ctgatccccg ctccatcaca gcctgagcac gatctgaatc atccatagct
      121 tttccttact tcgtctttcc tgccggaaca gcaggcgttc cggccatatc agtaaattca
      181 tgttgtcgcc gcgcctccag acgacgaata ctggccttgt ccacgttgca gttcttgatc
      241 accgccagca gttgcaggtt gtaagtgacc gatgccccaa acgtgaacgg ctccggcgca
      301 ggaggcacca ggcagtcagc cagccattca gcggggatcg gcaccggctt cacaggcacg
      361 tatttcacag acggcccggc gcacccgatc agccacatca tcaggaacag gggcagaggc
      421 cgcaggcact gctgccagcg accggcggac aacgctctgc tgcccctcgc tgtgctgaat
      481 gttctgttgt ttttcattcg tggccgcctc actgatttgt gaaatgagtt gcagggtgcg
      541 ttgctggttt gccagcacca gacgggcttc gtcccgttcg cgcacaagca catgattatt
      601 gtcctcaagg cgctgcgtct tctggtgcat tccccatacc acggcagcca gcacgctcat
      661 cacccccagc gccgccatac agcctccggc aatcagggcc ggtaagggat tattcaccgc
      721 agccacagca caccccgcag cagcacaacg gcacacagca gcgcaatcat gagcggtcgc
      781 cagaagcggt tcagactttc ggcaaatcgt agcgacataa ccattcatcc gccgaacggc
      841 gggcctcaag accggggagt ttcaccccct tcgaataaat ccagcggatg tactgcttac
      901 aggacgcggg catctgcccg gcattgatca ggcgcagaag cgtggagttg cggaagttcg
      961 tttcaccagc ccagaagatc cacgaagcca gcgccaccgt ctgaccacgg gtaagcggca
     1021 cctgaacacg ccgatcaatg gccgcaaagg cccatttcat gtccttttcc agtagttcca
     1081 ggcattcttc atccgtcagg gtcatacccg gtttcacatc cgggccggta tggccgtaac
     1141 agatggttgg tgtaccggtg ggatcgatat acgtggagtt ttctttccct tcccagtaac
     1201 cggtgtaatg cgtggtaatg gtgaacgtcc cggcaccggc cagaacaagg gcaatcagct
     1261 ttttacgcag tggtgcgggg agttttggca ttgttacgct gtctcatgat gttctctgag
     1321 tcagcgtaac ggtgaaggaa aggcggcagg ctttgcggaa tatcagaaaa gtggcggttg
     1381 cgttcttgcc agatgcagac ggcgctgttc gcgtatgacg gtataaatct gggtttctga
     1441 catcctgtaa tggcggcgca gggtttcaat tttttcgccc tggctccagc gggaaaaaat
     1501 ctcattattg cgcagttcgg tgaaaaggga ttcaccgacc gggagataat aaccacgccc
     1561 gcccatgtac ccggcctgtg cggccgcaac tttacgggca agcattcctg cctgagcagg
     1621 ttcaatcccc tgacggtgca gttcagcact gatcacatca accaggtccc gcagcgtgcc
     1681 gggccagttc tttttcagct catcatcagg aatgtcatcc agacggtcaa tcagcgcgtg
     1741 cagctgttcg ctgtcaccaa acatgctcat ctgtgtttca gccatacccg cctccggtca
     1801 tcattacaca gggcaagttt aaaataaaaa tcccgcgctg tggcgggatt tagaggcgct
     1861 gtaaatcata acccgaaatg agctgtgtat ttattgatca ttttccggtg taactatatg
     1921 ataaagatcg tttttatttt taaaaataca aaccctgaag gagtttttct tatcaaaaag
     1981 cttgtgcata ttgcgggtta ttgttgtttc atcaacatga aaaccaatta tcccaactct
     2041 taaagcacac caggcttgct ccagtgcaaa aacaaataat ttcagtctgc ctgacagcgt
     2101 tccaccggaa taaaggaaga tatccatgaa acaacctcca tctttgcttt tatcgtgccc
     2161 accagaactg gcccatgaac tcaatgatct ggcgaaagag tataaactta caaatcataa
     2221 tatagaagca gcaacactta gtgctgccgg aagcgatatt gtattgatcg ttctggccgc
     2281 agcttcatgc gatgggttct ggcaggcatt agccagtatg tttcagacgt ggttacatcg
     2341 taaaccaaca aaacaaacac ccccggaaaa gaaagttatt cgtagcgtca ttgttcgtag
     2401 ctgggaacac tatcaggaga caactaaaca aactgaattt tataactttg atgctgatga
     2461 gctggctaaa ttgatagcgt tatatgaaaa accagatgaa acaattggtt gcataatccc
     2521 gtcagaaacg gaaattcagg aaaaataaaa tcccgcactg tggcgggatt tgggttaaca
     2581 ggggaatgat ttttcataca gcctgcaaac gcgctcataa ctcatcactg cacgctccga
     2641 catatccagg gctgccagca ttttgcggcg gtgccagcgt ttgagacgct ccagcacatc
     2701 agaggccagc tcggggtgtt gctccagcca ctgccagtta gccacacctt cgccgccgtt
     2761 ctgcgcggcc gtctgcgatt tcacccagcg gttaagggcc gtttccgcac catcagaaat
     2821 aaagccctgc cggtgcatca ctttccagat ggcgcgaatt ttggcggtca ccgtacccgg
     2881 ctttaaggcc cggttaaccg gtttctgacg cactttaaaa ccgcgttttt tgaaaacatc
     2941 cagtacgcgg gataactcat ccggtgacat atcccggcaa ctggctttac cggtggctgc
     3001 cagcagtgcg gatgtatagg tttcatcatc cagagcaagc tcgcgttttg ccacatgaat
     3061 ggcagatatc agttttcctc tcataccgta tttacctttt tacggacatg gaaagccttt
     3121 tctggctcat tccaccggat atgatgcgtt ggcggaataa tcattatgtg ccagccatct
     3181 ctggttgtta ttcggcagaa aaactcaccg ctgtataaca caatggcctg ctgaataagc
     3241 gcatccgcag ccagctcaag cagatatctt ttatcaaaaa ggctgataat tttccggcat
     3301 aaccaccggg tgataacaaa tgcgccgaaa aacgatgtga tatataaaca ccagaacagc
     3361 agagaaatat tgtccgttat ttgctcatcc attgtgtatc tcccgtaatt ccggtaaagc
     3421 cgaaatatgg caccagtgcg tcacctgtcc atcctcatct tcaaggcagg ctccggtttc
     3481 ttcatcaaca aaaaaacctt catacccgat atggccgatg cgcagctcct gtcggtgacc
     3541 gtactgaaca atcagtacaa cagcctccat atcaggcggg aaagcatcat tcagtgaatg
     3601 ccagggataa ccgcgcaaat ccccgaaata aaccccggtc agggttccgc cacagaccgg
     3661 catcccggcg gcacatacca gaggttcgtc tgattcaaca aataaggtcg ttatcatggc
     3721 gttaactccc gtaatacagc gtccccggca gtccggcgat ggtgataaat aaaattcacc
     3781 gtactctcgg ataattcaaa tttctcgcct atttcccgga aggtaagacg tcgtggtgat
     3841 tcagcatccc gtaattcacg aataagatga acatcatcgt caggtatacg ggtgaagggc
     3901 agcaattctc cgtatttttt catgcttacg ccaatatatc gcgcccgatt gtggacagaa
     3961 tcccggtctc gccccagata ttctcctatt tgcttaccgg tcatcgttct ggcatttttt
     4021 cggataaatt tatcttcatc atgtttaaat cttggacgtt tgcatttgag caactccggg
     4081 taagtaccgc gtaataaaac aatccggtta tgtacacccc agaaacttct ttttaccctg
     4141 acagcaatat tcttaaccgg ggtggatgga taaagcgcaa tcagcagcgc atcttcttca
     4201 ggtgtccacg cacggacatg agcaggtgcc tgaccttttc gccccatggg ttgtaaaatc
     4261 atcatacatc accccctgac tcgcttttca gcagcataaa actcatcaga cagaatttcg
     4321 gtcattttct gatttgcggg ttttacgccg cactcaaggt aaatcacatc gtcaacgcag
     4381 aaccacttca cagggccaaa catgattgcc gagaaatcaa tacccagcca gaacaacagg
     4441 gcatcggttc tggcatacgt gacgggcgaa tattcacgcc acagaatgtt cagttcatca
     4501 gaggcaacac gcagggtttt agggatacgt gatgtgcgcg gcgtacagct ccagccattc
     4561 gacgcagttg gttttcgcca taagtcgcga tgaaaaggat atttgtcatc cataaagcgc
     4621 agccccttaa aacaaaagcc actgatactg aattcaaata ccgaccggca cgcaacattc
     4681 agcacggcct caaggcgttt cgcctcatct tttaccgtct ggcagtcctg ctggtatttt
     4741 tgccacgcag ccagcgcggc agggtttgat gttttaaaga acattactct acctccgcag
     4801 aaatcgcggc aggcgcagcc gtccggtcaa caatcaggta acgcagaaca tgttcggtga
     4861 tattccagcc gcttaaaccg cagataatga cgcccaggcg aatgtcgata tacgccatca
     4921 caaactgggg cacgccttct ttcatggcct gctcatcaac ttcagccacc acataaacgg
     4981 tttcacaggt cagaacgccc tgagcaaatt cccacgccat cgacggaata tcatgcccct
     5041 caatccacgg cagggatttt ctgaagctgc accactgaac atcatcagcc gcaccttcct
     5101 ggctgcattt gcgacgaacc ggctgcaccg ggcggggacg cggaatatca taaaaaccgt
     5161 tacattccgt cagcaccccg gcatcgaccg catcacgcag aaaataaacc atcgaggatg
     5221 gcggcatatt cattttttca gccagaatgc cgcaggtcag acgcccgtaa atactcagcc
     5281 agtttttaac cccttcaagc acttttgcat caatcatgaa ttattcctcc gtcagcgcca
     5341 catattcaga atgtttaatt tcctggcatt cttcaggaat accccctttt atttccctgc
     5401 cataagcgcc tactttaaac accacgcaca gggcatcacg ggagagcgac ggggtccacc
     5461 aggagtccgt gttaaacatg gaggggattc tcattacccc cagcttttca cagagccaga
     5521 caaggaaatc aggacgcttt cttaactcac gttcagcggt ttcaatcatt gcggcaatcg
     5581 ccggaccttc agggcatgac tcatcaggtc tggcaataaa gcacggtccg tccccgataa
     5641 aagccacatc atcgcgctta cgccagcccg caggcagaga accagaaaaa taaaactcac
     5701 tgattacgtc catggcgaac ggctttaagc gcaatccggc agcgcctgta tttttcagta
     5761 atgcccgaag aatactgttg cgtagcggta aaacagtctg ttgatatttg gtgaaatatt
     5821 tatttgtctg tacacggtct aatttgaaat aaaaatatgc catatttcct gttccttatt
     5881 ttcagtttac aggcgcaggc agtcccctga cgcgagcgcc ataattaaaa cgaagtgata
     5941 tttaaagatt taatgcggtg ttatttattc agtccggcgt cctgttcaaa aggctcgaca
     6001 tgaaaacttt cagcaccttt attcacctta ataccagcaa cccctttcac cgcatccggc
     6061 tccgccagga cagcttgctt attcacttcc tctttcgtgc ggatgaagcg ctcaagcccc
     6121 atacgacgca gcatttcaat cacaccttcc acatcacgac tgacgctgca tgatggattt
     6181 cccagccgcc atgacaccgt tccggtggtc agattggcag ttttggtttc gccgccgttc
     6241 gtcagctcat tgcggttggt tttacaccag tcatgaatcc ccttaaaaag cactttgatt
     6301 tcttttttaa ggttttcaat ctgcggcgta taacgggcgg tgatttctgc cacttcatca
     6361 ttcattgccg tttccaggcg cagcgcctct cgctgaatat cgcccagggt gcggatatcg
     6421 cggctgacct cttcgcgggt ctgtggtgcg gcctcggctg cggcctttaa ttttgtaaca
     6481 cgtttaacca ttttgttttc cttattgcag tgcgcctgat gctttatgca cacggtggcg
     6541 tttaatgatg gcatccgccc caaaagcctg tacagatgcc tgaaattccg ctgagagaag
     6601 ctgaataatt gcaggactgt gatactggag aatacttgca taaacgtcac agagtgacgt
     6661 cgggctgttc tccgtatcac gttctgtttt cacatccact ccaatctttt catgcacttc
     6721 aacacagtca ttgcctgcca gcttttcgtc atgcgaaaca tgctcaaatt caaaaataac
     6781 gcgtactttg ctcatggaat tacccctgct gattaaattc attaccgtta ataaccccgg
     6841 aagccatacc cctgtctttc atgaatgcac tggctttcat tgccacaagc tctgcgaagt
     6901 ttctgctttc tgcgagcata accaccccaa aaatcatggc ggggctacca tccttctgct
     6961 ttgctattcc ggttgaacgt atatccagac ttacccccat gtgcgtgctt ccatcagcgt
     7021 ttttacggat aacgttctcc ctgtattcga aaataatgcg aacaacttta ctcatcctct
     7081 ggtcctcgtt ttgttcggat aactgacccg gcgcctgacc tgatgacaga gccgaaccat
     7141 gaagtaactt catacttccc agtaaaccgt gcaaccgctg atgcgggtgg ccttcacacg
     7201 gcggcgcagg ccgttggtct taacggtgat ttcaatttcc tttccccccc cctgaacctt
     7261 atttaccggc ggcgtggttt tcagtgcaaa gcgttgcggg tagcgtttac tgcgctccag
     7321 taccgcaccg gcaagctcgg tcaggcgacg ggcgttattc agtgaatcaa acaaattcat
     7381 aggtgaactc cggttgtatc agtaatggat tcatgcgcga tgcagccagt tccagcagga
     7441 ggctccgatc aacctcaccc atccccggaa tttttcgggt ttcgccacgc atgattgccc
     7501 gaaggatgga gtaaccgtgt cgccccccct ggcgataatc cccctggatc agaaaaccaa
     7561 agcgcaggct ttcccagaca tgatggttaa agtatccaac ataaaaacgc tgccggacag
     7621 attcacactg ctggtctgca taccatagat acaggtcgat aaacagaaaa cacacaccgt
     7681 caccgttcat caattcaaag atataatcac gaacctcaca gtcattgttg ttaaacgcac
     7741 gaaaactgtg gagaacattg atttcctgct gtatctgcgt attcatataa cacctcgttt
     7801 atctgacttg ttttgcacca acagaacggc atattccgct gtaaacgtct gtcgcacctt
     7861 cgcgatcaag tccggcagat atcaccattc gtgtgcggtc attaaattca aaaagtaaat
     7921 caccacattc tttattttcg gccagtgaaa tcactttcac gttatcgaca ttcaccaggt
     7981 aaaagcgtcc gtaaatatca ggaatattaa atgcagccat aatcacacct gtgagagtaa
     8041 ttcagggttg gtataaacct ctttaaaggc cgcattaata tgtttttctg tcagcgccgc
     8101 gccttcaccg ctggctgtga gccacgcctg gttaagcgta tgtgtaagaa cacgtaacgc
     8161 accgggcttt tcagcgatag cctgcatcac gaccagctct gcctcaccac tgatacccca
     8221 tgccctggca atggccagca catctgcctt tttggccttg cgaagttgtt ttgtacgggc
     8281 aagacggctg aacaggcgcg ataaatcatc aaaggcgcgg cgtccacctt taaacagtcc
     8341 gcgcgggtta ccaataagca ccatcccgat ccccgtggcg tcctgaattg cccggagttg
     8401 ctccagaccg tcaataccaa gatgatccgc ctcatccaca atcaccagtc cacgcgttcc
     8461 catcagacgg cggcggatgg cgcgggataa tgcccctttg ttcgcgcggg tgtaatcaat
     8521 ccccagcgca tcggccagct ccagcaaaca ctccgtgacg ctggagtgcg cgggtgacag
     8581 ggtaatcatc caggtgtttg gttgctcctg gcagtaatta cgggcggtgg ccgttttacc
     8641 cacacccggc acgcccacaa tgacgttaat acagcccatc aggcgaaccg cctgaaacag
     8701 cgcgcgcagc tcctggactg tctgcgtttc cacaaactgc ggcggttccg gcagtgcgct
     8761 ttgtttattc cagttctcat accaggaacg cagggaagca gccacagcag cgttatcgcc
     8821 tttatatttt cccttacgga aagccgataa tgtgccgtcg gaaattcccg cctctctggc
     8881 gatggcatac tgtgtcagta cgccgccatc aataagttca tcaatggtct tgattacatc
     8941 gttaatatcg gtcattattc acctctcgtt tatgtgaata tctttgtttc gttaaataac
     9001 ctgagttgcc gctcgggtta tttttttatt tcaggccagc gggtcatttt cttttaattt
     9061 cgcttccagc agctgcaatc cccgctggaa attacgctcg tattcttcat caggttcatc
     9121 atcaacggca ggttgctgaa cggccaccgt attacctacc gggcgataga tgttttccag
     9181 ccagggctcc tgcggtttgt gctccagcac gttgaccacc tcatcctcgg catcacggat
     9241 tttttcctct gcgcgtttac gcatgccttt aaggcgttgc tgctgtttgt agtattccgc
     9301 gctgaccggg aaggcttcgc gtttattgcc gtcccatacc gcctcgcaaa tcacgctgcc
     9361 atccagtctg cgtacggtaa ttcgctcagc atcatgaatg tcatagctga taagcacctt
     9421 gcggccatgc tcgtcacgca gctccggcgc gtagtaaata ttattcagcc agcgtatttc
     9481 acagcgtctt acagggcgct tcaccatcgg ccggaacata tcccgcagct caacatcgga
     9541 cagccattca atttccgtgt cctcttccgc caggcgtttt tttctgaact ccgccgggct
     9601 gtaatgttta ccgttcggct tcatgggtaa ttcatcgtgc ggccggttgt tgtaccactc
     9661 aacaccatca cgaatggcat caatcagttc agaccaggac ggtaaatcac gcatcgctga
     9721 ctgctgccgg gcgttcagcc gtttgtcctg ttgcagggca gtgaatgccg agcgtaaatc
     9781 gcggttggtt ttgcgtaacg tctcgcgatc tgcacctttc ccgaaatagg tgcggtattt
     9841 acgggctatg cgcatcggta atgtgcggtt aagccgttcg ataatgcctc gcccctgcgg
     9901 attaccggca atcccggttg ggtgattaat ccccagtcgc ggcaggatcc ccacaacttc
     9961 tttatccagg atgtcggcgg tttccccgga accgttatcc gagtaataca gaaacggttt
    10021 gccgtgatgg cgaatgccgt gctgtatggc acccgctacg gcgaaaacat tttcagccag
    10081 gtcaaggctc cagcccacca caaagcgcgt gccgccatcg ataacaaagg ttacttccgg
    10141 tgcaaatggc cgcccgtgaa ccgggtgcgc gcatttcagc ttcatgccgt gaccgtcacc
    10201 aatccagaca taattcaccg gcattctgga ccagtcgcgg cgcgtgaatc cctcaagctg
    10261 acggtattca ctgccggtca cccggccttt ctgtttcacc acttccggca gtttcttcat
    10321 tgcgcggcga atggtgtcat aagagggcat gatatcgagc atataaggct catcagcgtg
    10381 ccggtgctgc cattcagcga caaaatcctc gtaagcctcg gtcattggtc gaccgtttga
    10441 ctggcgatac tgcgccagaa actcgggcag ccagttaata tcttcggctt ttatttcctg
    10501 gcgcttaccc ggtgccagca aaagcaggcg ttcggcggcg ttctgcgcct tgttaaaggc
    10561 tgcaatccag cgtttcagcg tgatttcact caacgcgcgg ctgtttccct ttttggcgtt
    10621 cgccgtctca accattgcca caatgcgctc gtccagttgc gaacgtgcca ggttgtcaac
    10681 gataaaccgg atagccttcg cgcagctgaa accgggttgt tgcgcgactt tcatcacttc
    10741 gctgacgatc gcgattcgtg catcagccac ctggcgctgg ttttcagtca gggcattaag
    10801 gcgttcgacc atcagttgtg gtgatccgcg atatgcctcc accgcatcaa ccactgcagg
    10861 cgagcgtctg gcctttgtca ccaccggagc cggtgattcg tcggcttttt gcgtcatcag
    10921 ttgcagggcg tagcgttcac gtaatgcctg ctgcgtcact tcaggtaaac agtcgatgct
    10981 gtattcaaaa cctttagtgc cttcctggcg gcgtctgctg gtttcgctgg ttgcatactt
    11041 cctgattgaa tagcggacgc cttgctccgt tcccggcatt cccggtaatc caaccagctc
    11101 tttaactgaa gcaaacataa ttacgcgacc ttacgaatgt agcggctggg ccaaatctcc
    11161 tcgggtggaa cacccaggca ttccgcaata attccctcat acttgggaac atggcggtat
    11221 accgcgttat acaggttgtt ctggcctaca ccagctttac gggccagcgc cctcatactt
    11281 ccttctctgg ctctgacttc ccccaagatt ctttgaggat gccaatcagg cttaacttca
    11341 tttctcgcca tgattcatct atcctaaaaa gttacgaaat ttgataacta aa
//
LOCUS       DAZFKF010000136        11316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFKF010000136 DAZFKF010000000
VERSION     DAZFKF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.138"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            178..720
                     /gene="ecpR"
                     /locus_tag="ACHKA1_004594"
                     /gene_synonym="matA"
     CDS             178..720
                     /gene="ecpR"
                     /locus_tag="ACHKA1_004594"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO5682150.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            795..1382
                     /gene="ecpA"
                     /locus_tag="ACHKA1_004595"
                     /gene_synonym="matB"
     CDS             795..1382
                     /gene="ecpA"
                     /locus_tag="ACHKA1_004595"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO5682151.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1440..2108
                     /gene="ecpB"
                     /locus_tag="ACHKA1_004596"
                     /gene_synonym="yagY"
     CDS             1440..2108
                     /gene="ecpB"
                     /locus_tag="ACHKA1_004596"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO5682152.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2134..4659
                     /gene="ecpC"
                     /locus_tag="ACHKA1_004597"
                     /gene_synonym="yagX"
     CDS             2134..4659
                     /gene="ecpC"
                     /locus_tag="ACHKA1_004597"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO5682153.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSMWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGPFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4649..6292
                     /gene="ecpD"
                     /locus_tag="ACHKA1_004598"
     CDS             4649..6292
                     /gene="ecpD"
                     /locus_tag="ACHKA1_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO5682154.1"
                     /translation="MRVNLLITMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6261..6971
                     /gene="ecpE"
                     /locus_tag="ACHKA1_004599"
     CDS             6261..6971
                     /gene="ecpE"
                     /locus_tag="ACHKA1_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO5682155.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7284..7613
                     /gene="ykgJ"
                     /locus_tag="ACHKA1_004600"
     CDS             7284..7613
                     /gene="ykgJ"
                     /locus_tag="ACHKA1_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO5682156.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7824..8438)
                     /gene="yagU"
                     /locus_tag="ACHKA1_004601"
     CDS             complement(7824..8438)
                     /gene="yagU"
                     /locus_tag="ACHKA1_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO5682157.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(8485..8694)
                     /locus_tag="ACHKA1_004602"
     CDS             complement(8485..8694)
                     /locus_tag="ACHKA1_004602"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682158.1"
                     /translation="MMMGAGLLLELYDAKTDKETCDCQLCITLQIYVTSNIKIGRNDG
                     AYILWRLDNHLVIQNNTVLYIYLVL"
     gene            8856..9545
                     /gene="paoA"
                     /locus_tag="ACHKA1_004603"
     CDS             8856..9545
                     /gene="paoA"
                     /locus_tag="ACHKA1_004603"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFO5682159.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDFSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9542..10498
                     /gene="paoB"
                     /locus_tag="ACHKA1_004604"
     CDS             9542..10498
                     /gene="paoB"
                     /locus_tag="ACHKA1_004604"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFO5682160.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPDAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10495..>11316
                     /gene="paoC"
                     /locus_tag="ACHKA1_004605"
     CDS             10495..>11316
                     /gene="paoC"
                     /locus_tag="ACHKA1_004605"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO5682161.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     SPNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALSKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPT"
BASE COUNT         2914 a         2980 c         2925 g         2497 t
ORIGIN      
        1 ttaaacctaa tcgaaataat taaaacttaa tctcgtttaa cctatattga tatgtgctac
       61 gtatcttatt tacttccgat ttactaaaga aactgaatgt acctgtaaaa attacaggtt
      121 tggaaagtag tgacatggca aaatgattac agcagggact atgaggttaa aaaccatatg
      181 gaatgtcaaa accgttctga taaatacatc tggtctcccc atgacgccta cttctataaa
      241 ggactatctg aactgattgt ggatatcgac agattaattt atctatcgct ggagaaaatc
      301 agaaaagatt tcgtgtttat caatctcaat acggattctt taactgaatt tataaaccgt
      361 gataatgagt ggttatccgc ggtaaagggg aaacaggtcg tattgattgc ggccagaaag
      421 tcagaagcct tagcaaatta ttggtattac aacagcaata ttagaggcgt ggtatacgct
      481 ggactgagtc gtgatattag aaaagaactg gcctatgtga ttaatggcag gttcctgaga
      541 aaagatatta agaaagataa aatcactgac cgggaaatgg aaattatccg catgacggcc
      601 cagggaatgc aacctaaatc gattgccaga attgaaaatt gtagtgtgaa gacagtgtat
      661 acccatcggc gtaatgctga ggccaagctg tactcaaaaa tatataagtt ggttcagtaa
      721 actccaggca agttagtttt aaaaaatgac tcactgggac atcacgtcct caattcaact
      781 cgggaagaaa tgcaatgaaa aaaaaggttc tggcaatagc tctggtaacg gtgtttaccg
      841 gcatgggtgt ggcgcaggct gctgacgtaa cagctcaggc tgtagcgacc tggtcggcaa
      901 cagccaaaaa agacaccacc agtaagctgg ttgtgacgcc actcggtagc ctggcgttcc
      961 agtatgccga aggcattaaa ggttttaact cacagaaagg tctatttgac gtggctatcg
     1021 agggtgactc aacggctacc gcctttaaac tgacctcacg tcttatcacc aacacattaa
     1081 cccagttgga tacctcaggt tccacactga atgtgggcgt ggattataac ggcgcggcag
     1141 tcgaaaaaac tggcgatacc gtgatgatcg ataccgccaa cggcgtactg ggcggcaacc
     1201 ttagcccgct ggctaacggt tacaatgcca gcaatcgtac caccgcacag gatggtttca
     1261 ccttcaccat catcagcggc accaccaatg gtaccaccgc agtaaccgac tacagcactc
     1321 taccggaagg catctggagc ggcgacgtta gcgtacagtt cgacgcgacc tggaccagtt
     1381 aatctctctg atgtaccagc aggggtagcc cccctgcttt attcctggac ggaattgtta
     1441 tgaaaaagca ccttctgcct ctcgctctgc tgttttccgg aatatctccg gcccaggcgc
     1501 tggatgtcgg cgatatatca tcgtttatga acagtgacag cagcacgctg agcaaaacga
     1561 tcaaaaacag taccgacagt ggtcgcctta tcaatatccg tctcgaacgg ctctcttcac
     1621 cgcttgacga cgggcaggtt atctcaatgg acaagccgga tgagttgcta ctcactcccg
     1681 ccagcttgct gctacccgcc caagccagcg aagtgatccg cttcttctat aagggacccg
     1741 cagatgaaaa agagcgctac taccgcattg tctggtttga tcaggccctc agtgatgcgc
     1801 agcgcgataa tgccaaccgc agcgctgtgg ccactgcttc cgcccgcatc ggcaccattc
     1861 tggtcgtcgc ccctcgccag gcgaactacc actttcagta cgccaacggc tccctgacaa
     1921 atacaggaaa tgcgacgctg cggatcctcg cctacggccc ttgcctgaaa gccgccaacg
     1981 gtaaggagtg taaagagaat tactacctga tgccgggcaa gtcgcgtcgt tttacccgcg
     2041 tggacactgc ggataacaaa ggacgggttg cactttggca gggtgataag ttcattcccg
     2101 tgaaatagat agctgtgcag atggataacg acaatgcctt tacgacggtt ctccccagga
     2161 ctgaaagccc agtttgcctt cggcatggtc tttttgttcg ttcagcccga tgccagcgct
     2221 gctgacataa gtgcgcagca aataggtggg gtgattattc cgcaggcctt cagtcaggcg
     2281 cttcaggacg gcatgagcgt cccgctctat attcatctcg ccggtagcca gggtcgccag
     2341 gacgatcagc gaatcggcag cgcttttatc tggctggatg atggacagct acgcatccgg
     2401 aaaatacagc tggaagagag tgaagataac gccagtgtca gcgaacaaac tcgacagcag
     2461 ctgatggctc tggcgaacgc cccgttcaat gaggccctta ccatccccct gactgacaac
     2521 gcgcagctgg atctcagctt gcgccaactg ctgctgcagc tggtggtcaa gcgcgaagcg
     2581 ctgggcaccg tactacgctc acgtagcgaa gacatcgggc agtccagtgt taacaccctc
     2641 agcagtaatc tgagctataa cttgggcgtc tataacaacc agttgcgtaa cggcgggagc
     2701 aacacatcca gctatctgtc gctgaataac gttactgcgc tgcgcgaaca tcatgtggtg
     2761 ctcgacggct cgctgtacgg gatcggtagc ggtcaacagg acagtgaatt atataaagcg
     2821 atgtatgaac gcgattttgc cggtcaccga tttgccggtg gaatgctcga cacctggaac
     2881 ttgcagtcct tagggccgat gaccgccatt tcagcaggga agatttacgg cctttcctgg
     2941 ggaaaccagg ccagctccac catcttcgac agcagccagt cagccacgcc agtgatcgcc
     3001 tttttaccgg cggcgggtga agtacatctc acccgtgatg ggcggctact aagcgttcag
     3061 aacttcacta tgggcaatca tgaagtggat acccggggtc taccgtacgg tatttacgat
     3121 gtggaagttg aggtgatcgt taacggtcgc gtgatcagca aacgcaccca gcgggtcaat
     3181 aagctgttta gccgggggcg cggcgttggt gcaccactgg cgtggcaggt atggggcggt
     3241 agctttcata tggatcgctg gtcggaaaac gggaaaaaga cgcgaccagc taaggagagc
     3301 tggctagcag gtgcctcgac ctccggctca ttgagtacgc ttagctgggc ggcaacggga
     3361 tatggatacg ataatcaggc ggtgggtgaa acccgtctga cgctgccgct tgggggagcg
     3421 atcaacgtta acctgcaaaa tatgctggcc agtgacagct catggagcaa catcgccagc
     3481 atcagcgcca ctctaccggg aggctttagt tcgatgtggg ttaaccagga aaaaacccgc
     3541 attggcaatc aattgcgacg tagcgatgcc gacaaccgtg caatcggcgg cacactcaac
     3601 ctgaactcac tgtggtcgaa gctgggcaca ttcagcatca gctacaatga tgaccgccgt
     3661 tacaacagcc attattacac agcagattac tatcaaaatg tctacagcgg tccctttggt
     3721 tcgcttggcc tgcgggccgg tattcagcgc tataacaacg gcgacagcaa cgccaataca
     3781 gggaaatata tcgctctcga tctctcgcta ccactgggca actggtttag cgcagggatg
     3841 actcatcaaa acggctacac catggcaaac ctgtcagcac gcaagcagtt tgatgaaggg
     3901 accattcgca ctgttggtgc caatctgtct cgagccatct ccggcgatac cggtgatgac
     3961 aaaactctca gtggtggggc gtatgcacag ttcgacgctc gctacgccag cggaacgctg
     4021 aacgtcaata gcgcggcgga cggctacgtc aataccaatt taaccgccaa tggcagcgtc
     4081 ggctggcagg gtaaaaacat tgctgccagc gggcggactg atggcaacgc tggggtgata
     4141 ttcaacaccg ggctggagga cgacggtcag atcagcgcca aaatcaacgg gcggattttc
     4201 ccacttaacg gcaagcgtaa ctatctccca ctctctccct atggaagata tgaggtggag
     4261 ttacagaaca gcaaaaactc actcgacagt tacgatatcg tcagcggccg caaaagccat
     4321 ctgactctct atccaggcaa tgtcgctgtc attgagccag aggtgaagca gatggttacc
     4381 gtctccggtc gtatccgtgc ggaagacggc acactgctgg ctaacgcacg gattaacaac
     4441 catatcggcc gaacccgaac cgatgaaaac ggcgagtttg tcatggacgt ggataagaaa
     4501 taccccacta tcgattttcg ctacagtggc aataaaacct gcgaagtggc tctggaactc
     4561 aaccaggcgc gcggcgccgt ctgggtcggt gatgtggtct gcagcggcct ctcatcgtgg
     4621 gcggcggtga cgcagacagg agaagagaat gagagttaac ctactaataa cgatgataat
     4681 ctttgcgcta atctggcctg caaccgcgct cagagctgca gtgagcaaaa caacctgggc
     4741 ggatgcaccg gcacgcgagt ttgtgtttgt cgaaaacaac tcagacgaca actttttcgt
     4801 cactcctggc ggggcgctgg atccgcgcct gaccggtgcc aaccgctgga ccggtttaaa
     4861 atacaatggt tcaggaacca tctatcagca aagcctcggc tacattgata acggttacaa
     4921 caccggcctc tataccaact ggaagtttga tatgtggctg gaaaattcgc cggtttcatc
     4981 tcctttaact ggcttacgct gcatcaactg gtacgcaggg tgtaatatga ccaccagtct
     5041 tatcctgccg caaaccaccg acgccagtgg attttatggc gcgacggtga ccagcggcgg
     5101 cgcgaagtgg atgcacggca tgttgtcaga cgcgttttac cagtatctgc aacaaatgcc
     5161 cgtcggcagc agctttacaa tgaccatcaa tgcctgccag acctctgtga actatgacgc
     5221 cagcagcggc gcacgctgta aggatcaggc ctccggcaac tggtatgttc gcaacgtcac
     5281 ccatacgaaa gcagcaaatc tacggttgat aaatacccac tcgctggcgg aagtatttat
     5341 caacagcgac ggagtaccga ctctgggcga agggaacgcc gactgccgga cgcaaaccat
     5401 cggcagccgt tcaggattaa gttgtaagat ggttaactat accctgcaaa caaacggact
     5461 cagcaacacc tcaatccata tattcccggc gatcgccaac tcgtcgttag cctcggccgt
     5521 cggggcgtac gatatgcagt tcagtctgaa tggcagttca tggaaaccgg tgagcaatac
     5581 cgcctattac tacaccttca acgagatgaa gagcgcagac tcgatctatg ttttcttctc
     5641 gagcaacttc tttaagcaga tggtgaacct cgggatcagc gatatcaaca ccaaagatct
     5701 attcaacttt cgctttcaga acaccacatc accggagtct ggctggtatg aattttctac
     5761 ctccaacacg ctgattatca aaccccgtga tttcagcatc agtattatct ccgatgaata
     5821 tactcagaca ccgtcgcggg agggatatgt tggcagcggc gagtcggcac tcgatttcgg
     5881 ctatatcgta accaccagcg gtaaaacagc tgccgacgaa gtgctgatca aggtgaccgg
     5941 acccgcgcag gtgattggcg ggcgctccta ttgtgtcttc agctccgatg acggtaaggc
     6001 gaaagtaccg ttcccggcga cactttcctt tattacccgc aacggagcta caaaaaccta
     6061 cgatgccggg tgcgatgata gctggcggga tatgaccgat gcgctgtggt tgaccacacc
     6121 gtggactgat atctctggcg aagtggggca gatggataag accacagtca aattttcgat
     6181 tccaatggat aacgccattt cgctgcgtac ggtagatgat aacggctggt ttggcgaagt
     6241 cagcgcttca ggagagattc atgttcaggc gacgtggcgt aacattaact aaggccctgc
     6301 tgacagcggt ctgtatgctg gcggcacctt tgacacaggc gatttcggtc ggcaatctga
     6361 cattttcgct gccgtccgag actgactttg tcagcaaacg tgtagtgaat aacaacaaaa
     6421 gcgcacggat ataccgtatt gccatcagtg ctattgatag cccgggcagc agtgaattgc
     6481 gcacccgacc ggtggatggt gaactgcttt tcgccccccg ccagctggcg ttgcaggctg
     6541 gtgagagcga gtattttaaa ttttactatc atggtccaca ggataaccgc gagcgctact
     6601 accgggtctc atttcgcgag gtccccactc gtaaccagac aagacgcagc ccaaccggcg
     6661 gcgaggtcag cacggagccg gtggtggtga tggataccat tctggtagta cgaccacgtc
     6721 aggttcagtt taaatggtcc ttcgatcagg tgaccggaac ggtaagtaac accggcaaca
     6781 cgtggtttaa gctactgatt aagccaggat gtgattcgac cgaagaggaa ggcgatgcct
     6841 ggtatctacg tccgggagac gtggttcatc agcctgagtt acgtcagccg gggaatcatt
     6901 atctggtcta taacgacaaa ttcattaaga ttagcgattc ctgtccggct aagccccctt
     6961 cggcggactg atacttatcc atcggtcgag ggaagaattc cacctcagag actcaaaaaa
     7021 cgtttaaatg atgtggaagc gatcgtcaat gtcacctgta acgatgggct ttctcatatg
     7081 cacaccatag tgttcaggag acacagaaag gtatcaggct gcctgctaac gagtggttta
     7141 ttcacgaaat agtacgtaaa atcggtaacg gctggaaatc attcaatact cacactatcg
     7201 aaagtacacc agccaaccgc ggtacgttct tacatacgat gtaccgctgt tctctttacg
     7261 atttatagct gtactggtga attatgagca atctgaatcc atgcatgacg tgtggtgcct
     7321 gttgtgcatt tttccgcgtc tctttttact gggccgaagc cgacgatgct ggcggtacca
     7381 ttcccgccag gctcactgaa caaatatccc cttttcaccg atgcatgagc ggtaccaatc
     7441 agaaaaaccc ccgatgtatt gcccttgcag gaaccccagg caaaaatgcc tgctgcacga
     7501 tatataaaaa tcgatcgtcc acgtgcagag aattcgccat gtctggtgag aacggagaag
     7561 tcaatgaggc ttgcaatcgt gcaagggcta aatacgggct gacaccgtta taaatataca
     7621 ataattaatt gcactgcccc gccagcgata atagcggggc ttcgttttca gggacaacaa
     7681 aacccgaaac ttcttgtatt tgccagcaac aatgcctctc ttctgtagcg ttcttcggat
     7741 ctacatcatc ctaaagtagc gcgatatact cactgactat ctgaatcaga atataaaaag
     7801 caatgttttt atcattcaat gcattatctg tttgagccta aggggatctc agggtctggc
     7861 tcatgagtaa ttctgtttcg taaatctctg cgaataattt caatggacca gaaccagact
     7921 aaatgtccaa aaatttcaga aacattctca taccacggga gatcaaaaag aggtggtgtc
     7981 agtcccatga gagggaatga aatcatatga acaaaaagtt gggctaaagc acctgccagt
     8041 aagccctgcc agagtttaat ttttggaaat acttcagcga ccacacaata accgacagca
     8101 aacactatcg aaaagataat gtgcgtaaca ccaacccagt taaagacatg cccggcaaag
     8161 gtataaacag ccgcattggg atctgtcagc cccagccagt ctcgaagaaa aatatacgga
     8221 ggattgagaa aattacgcga gcaatcaatt tgcccagcag ccctgattaa tgattccggg
     8281 ccacacgctg cattaaacat atccaccggg ctacgtggcg gcaatggaac ttcagccccc
     8341 cacttcacaa atgcggaaac aacgccagca atcagcccaa tgaatgcagc aagaccataa
     8401 cgtctgcggt tcggtggagt ttgttcaaat atattcatat ctaccctgct tgtaccatta
     8461 tgttatacac ctctttagga gtattcataa aacaaggtaa atgtaaagaa ctgtattgtt
     8521 ttgtataaca agatggttgt ctaatcgcca aagaatataa gcaccatcat ttctccctat
     8581 ttttatatta gaagtgacat agatttgcag ggtgatgcag agctgacaat cacaggtttc
     8641 cttatcggtt tttgcatcgt acaactcaag caataaacca gctcccatca tcatcaacat
     8701 cccggcgaac atgaatttat tagccgggct catagctgca ttcgcgtttt aagaataatc
     8761 ctcctgctgt cgccgactat gcttaacgtt taaaaaagca tcagcactct cgcaacgcac
     8821 tcttattttc ccctttagaa taccggaggc ctggtatgag caaccaaggc gaataccccg
     8881 aagataatcg ggttgggaag cacgagccgc acgattttag tttgacccgt cgcgatctga
     8941 ttaaagtgag cgccgcaacg gcggcgaccg ccgtggttta tcctcattct acgcttgcgg
     9001 caagcgttcc ggcagctaca cccgcgccag agataatgcc cctgacactt aaggtgaacg
     9061 gcaaaacgga gcagcttgag gtggataccc gaaccacgct actggacgct ttgcgtgaaa
     9121 atctgcattt gatcggtacc aagaaaggtt gcgatcacgg acagtgcgga gcctgtaccg
     9181 tgctggtcaa tggtcgcagg cttaatgcct gcctgacgct ggcagtcatg catcaggggg
     9241 ccgagatcac caccattgaa ggcctgggtt caccagataa tcttcacccc atgcaggcgg
     9301 cctttatcaa gcatgatggc ttccagtgcg gctactgcac ctccgggcaa atttgctcat
     9361 cagtagcggt gctaaaagag attcaggacg gcattcccag tcacgtcacg gtcgatttgg
     9421 tttcccctcc agaaagaact gccgatgaga tccgtgaacg tatgagcggc aacatctgtc
     9481 gctgtggtgc atacgctaac atccttgccg ccattgaaga cgctgcgggg gagataaaat
     9541 catgaaggcg tttacctatg aacgagtgaa caccccggcc gaggcggcac ttagcgctca
     9601 gcgcgtaccc gacgcaaaat ttatcgcggg cgggaccaat ctgctggacc tgatgaagct
     9661 ggaaattgaa acgcccaccc accttatcga tgtgaacggc ctcgggctcg ataagattga
     9721 agtgaccgac gcgggtgggc tgcgcatcgg cgcactggta cggaacaccg acctggcggc
     9781 tcacgagcgc gtgcgtcgtg attacgcggt actctcccgc gccctgctcg ctggcgcgtc
     9841 tggtcagtta cgcaatcagg caaccaccgc aggtaatctg ctccagcgca cgcgctgccc
     9901 ctatttttac gacaccaatc agccctgcaa taagcgcctg cccgggagcg gctgcgcggc
     9961 gcttgaaggc tttagccgtc agcacgcggt ggtaggcgta agcgaagcct gcattgccac
    10021 ccatccgagc gatatggcgg tcgcaatgcg gttgctggat gcggtggtgg aaaccatcac
    10081 gccggaggga aagactcgca gtatcacact ggctgatttt tatcacccac cggggaaaac
    10141 gccgcacatt gaaaccgccc tgcttcccgg tgagcttatc gttgcggtga cgttacctcc
    10201 gccgctcggc ggaaaacata tttaccgtaa ggtgcgcgat cgcgcctcct acgcctttgc
    10261 cctggtatcg gtcgcggcga ttattcagcc tgacggcagc gggcgcgtcg cgctgggcgg
    10321 agtagcacat aagccctggc gcattgaggc tgcggatgct cagctatccc agggggcaca
    10381 ggccgtgtat gacgcgctgt tcgccagcgc ccatcccacc gctgaaaaca cctttaaact
    10441 cctgttggcg aagcgaacgc ttgcctccgt actggctgaa gcgagggcac aggcatgaaa
    10501 tttgataaac ccgcagggga aaacccgatc gatcagctga aggttgtcgg tcgtccccat
    10561 gaccgcatcg acggaccgct gaaaactacc ggcacggcac gctacgccta cgaatggcat
    10621 gaagaatccc ccaacgccgc ctatggctat atcgtcggtt ccgccattgc caaaggacgc
    10681 ctcaccgccc ttgatacgga cgccgcgcaa aaagcgccgg gcgtactggc tgtcattacc
    10741 gccagtaacg ccggggcact cagtaaaggc gacaaaaaca ccgccaggct gttaggcggc
    10801 cccactattg agcactatca tcaggccatt gcgctggtag tggccgagac cttcgaacag
    10861 gcgcgagcgg cggcctcgct ggtgcaggcg cactatcgcc gtaataaagg agcttactcc
    10921 ctggcggacg aaaaacaggc cgtcagtcag ccgccggaag acacgcccga caaaaacgtc
    10981 ggtgactttg acggggcttt ctcctccgct gcggtgaaga ttgatgctac ctacacgacc
    11041 ccggatcaga gccatatggc gatggagccg catgcctcga tggccgtctg ggatggaaat
    11101 aagcttactc tctggacctc aaatcagatg attgactggt gccgcaccga tctggcaaaa
    11161 acgctgaaag tgcccgtgga gaatgtgcgt attatctccc cgtatatcgg cggagggttt
    11221 ggcggcaagc tgttcctgag aagcgatgcg ctgctggcag ccctcgccgc ccgagcggtg
    11281 aaacgtccgg ttaaagtgat gctcccccgc cccact
//
LOCUS       DAZFKF010000137        11180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFKF010000137 DAZFKF010000000
VERSION     DAZFKF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.139"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            29..571
                     /locus_tag="ACHKA1_004606"
                     /pseudo
     CDS             29..571
                     /locus_tag="ACHKA1_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            864..3014
                     /gene="btsT"
                     /locus_tag="ACHKA1_004607"
     CDS             864..3014
                     /gene="btsT"
                     /locus_tag="ACHKA1_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO5682162.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            3064..3267
                     /locus_tag="ACHKA1_004608"
     CDS             3064..3267
                     /locus_tag="ACHKA1_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO5682163.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            3278..4234
                     /gene="yjiA"
                     /locus_tag="ACHKA1_004609"
     CDS             3278..4234
                     /gene="yjiA"
                     /locus_tag="ACHKA1_004609"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO5682164.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILMTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(4280..5194)
                     /gene="mrr"
                     /locus_tag="ACHKA1_004610"
     CDS             complement(4280..5194)
                     /gene="mrr"
                     /locus_tag="ACHKA1_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine/cytosine-specific restriction
                     endonuclease Mrr"
                     /protein_id="HFO5682165.1"
                     /translation="MTVPTYDKFIEPVLRYLATKPEGAAARDVHEAAADALGLDDSQR
                     AKVITSGQLVYKNRAGWAHDRLKRAGLSQSLSRGKWCLTPAGFDWVASHPQPMTEQET
                     NHLAFDFVNVKLKSRPDAVDLDPKADSPDHEELAKSSPDDRLDQALKELRDAVADEVL
                     ENLLQVSPSRFEVIVLDVLHRLGYGGHRDDLQRVGGTGDGGIDGVISLDKLGLEKVYV
                     QAKRWQNTVGRPELQAFYGALAGQKAKRGVFITTSGFTSQARDFAQSVEGMVLVDGER
                     LVHLMIENEVGVSSRLLKVPKLDMDYFE"
     gene            5382..8894
                     /gene="hsdR"
                     /locus_tag="ACHKA1_004611"
     CDS             5382..8894
                     /gene="hsdR"
                     /locus_tag="ACHKA1_004611"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     endonuclease"
                     /protein_id="HFO5682166.1"
                     /translation="MMNKSNFEFLKGVNDFTYAIACAAENNYPDDPNTTLIKMRMFGE
                     ATAKHLGLLLNIPPCENQHDLLRELGKIAFVDDNILSVFHKLRRIGNQAVHEYHNDLD
                     DAQMCLRLGFRLAVWYYRLVTKDYDFPVPVFVLPERGENLYHQEVLTLKQQLEQQVRE
                     KAQSQAEVEAQQQKLVALNGYIAILEGKQQETEAQTQARLAALEAQLAEKNAELAKQT
                     EQERKAYHKEITDQAIKRTLNLSEEESRFLIDAQLRKAGWQADSKTLRFSKGARPEPG
                     VNKAIAEWPTGKDETGNQGFADYVLFVGLKPIAVVEAKRKNIDVPGKLNESYRYSKCF
                     DNGFLRETLLEHYSPDEVHEAVPEYETSWQDTSGQQRFKIPFCYSTNGREYRAAMKTK
                     SGIWYRDVRDTRNMSKALPEWHRPEELLEMLGSEPQKQNQWFADNPGMSELGLRYYQE
                     DAVRAVEKAIVKGQQEILLAMATGTGKTRTAIAMMFRLIQSQRFKRILFLVDRRSLGE
                     QALGAFEDTRINGDTFNSIFDIKGLTDKFPEDSTKIHVATVQSLVKRTLQSDEPMPVA
                     RYDCIVVDEAHRGYILDKEQTEGELQFRSQLDYVSAYRRILDHFDAVKIALTATPALH
                     TVQIFGEPVYRYTYRTAVIDGFLIDQDPPIQITTRNAQEGVYLSKGEQVERISPQGEV
                     INDTLEDDQDFEVADFNRGLVIPAFNRAVCNELTNYLDPTGSQKTLVFCVTNAHADMV
                     VEELRTAFKKKYPQLEHDAIIKITGDADKDARKVQTMITRFNKERLPNIVVTVDLLTT
                     GVDIPSICNIVFLRKVRSRILYEQMKGRATRLCPDVNKTSFKIFDCVDIYSTLESVDT
                     MRPVVVRPKVELQTLVNEITDSETYKITEADGRSFAEHSHEQLVAKLQRIIGLATFNR
                     DRSETIDKQVRRLDELCQDAAGVNFNGFASRLREKGPHWSAEVFNKLPGFIARLEKLK
                     TDINNLNDAPIFLDIDDEVVSVKSLYGDYDTPQDFLEAFDSLVQRSPNAQPALQAVIN
                     RPRDLTRKGLVELQEWFDRQHFEESSLRKAWKETRNEDIAARLIGHIRRAAVGDALKP
                     FEERVDHALTRIKGENDWSSEQLSWLDRLAQALKEKVVLDDDVFKTGNFHRRGGKAML
                     QRTFDDNLDSLLDKFSDYIWDELA"
     gene            8971..10560
                     /gene="hsdM"
                     /locus_tag="ACHKA1_004612"
     CDS             8971..10560
                     /gene="hsdM"
                     /locus_tag="ACHKA1_004612"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     methyltransferase"
                     /protein_id="HFO5682167.1"
                     /translation="MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCKETG
                     QEAEYLPVGYRWDDLKSRIGQEQLQFYRNLLVHLGADNQKLVQAVFQNVNTTITQPKQ
                     LTELVSNMDSLDWYSGAHGKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTII
                     HLLKPQPREVVQDPAAGTAGFLIEADRYVKSQTNDLDDLDGDTQDFQIHRAFIGLELV
                     PGTRRLALMNCLLHDIEGNLDHGGAIRLGNTLGSDGENLPKAHIVATNPPFGSAAGTN
                     ITRTFVHPTSNKQLCFMQHIIETLHPGGRAAVVVPDNVLFEGGKGTDIRRDLMDKCHL
                     HTILRLPTGIFYAQGVKTNVLFFTKGTVANPNQDKNCTDDVWVYDLRTNMPSFGKRTP
                     FTDEHLQPFERVYGEDPHGLSPRTEGEWSFNAEETEVADSEENKNTDQHLATSRWRKF
                     SREWIRTAKSDSLDISWLKDKDSIDADNLPEPDVLAAEAMGELVQALGELDALMRELG
                     ASDEADAQRQLLEEAFGGVKE"
     gene            10557..>11180
                     /locus_tag="ACHKA1_004613"
     CDS             10557..>11180
                     /locus_tag="ACHKA1_004613"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO5682168.1"
                     /translation="MSLKNIPTNWCVTTIGDINDYKSLTFNPKSYQEDSFELYSVPSF
                     ASGSPEILLGKDIASNKQYIQFKDILLCKINPRINRVWCVKEKKEHPQIGSSEWIVIR
                     QPLIHEGFLAYFLQTQDFRDLLCSDVSGVGGSLTRAQPKRVATYNVPIAPLPEQKIIA
                     EKLDTLLAQVESTKARLEQIPQILKRFRQAVLGAAVSGKLTQNWRENN"
BASE COUNT         2822 a         2946 c         2886 g         2526 t
ORIGIN      
        1 tgtggaattt taagaaagga catctgaaat gttaaaccgt gacttcgatc atctctcaac
       61 gctattacat ctcttttttg aacgtgagga tatttgccaa aagcttaata ttattgacca
      121 aaacaatatc accggttggg aagtgtggtt ccaggtagag tttgctaata tgctctgctc
      181 aacagaccat gaatggtggc gtgaacaagc actttcctgt gatatgcgca aaaaacctga
      241 gcgcccgaca ctacgcacag atttcttact acgtaaaaaa ggctgggcac aagatagcta
      301 tatcacgctt gaaataaaac aaaacagaga cgcgaccagt tgtgttaaaa atatgatcgc
      361 agacttagaa aaaagcgcaa aaataaaacg ttccgaactc gatttacgca gtttctgaac
      421 cttgggtatc acccctatga tagacaggga aaggctggac tctctcatcg attgctattt
      481 agatgagaaa ttttatctta ctaaatctcg taaaaaacac gttttactta aagaaataaa
      541 taacacacca tattgctata ttgttattta atatttatag catgaatata atttttaaat
      601 gagttacaca accgagggct aaaaatcgct tcggttgtga tcatcatcac ataaataacc
      661 ttcataaacc ttgctcaaaa ccgctcaacc gcaaaactga ccgcttacgt ccctaaaata
      721 accactcagt tatttacctt actttacgcg cgcgtaactc tggcagcatc actgcaggat
      781 agcggtcaat ttacctcctc aaacgcaacg caaacctaga acggcttcgg ccaactatta
      841 atcaatacat gccaggtttt actatggata cgaaaaaact attcaagcac ataccctggg
      901 tgattctcgg aatcatcggt gcattctgcc tcgcggtagt tgcattacgt cggggggagc
      961 acgtcagtgc cctgtggatc gtggtcgcct ctgtgtcggt gtatctggtg gcgtatcgct
     1021 actacagttt gtatatcgcc cagaaggtga tgaaactcga ccccacgcgc gcgacgcctg
     1081 cggttattaa caacgacggt ctgaactacg ttccgaccaa ccgttacgtg ttgtttggtc
     1141 accacttcgc cgctatcgcc ggtgctggtc cgctggttgg tccggttctc gccgcgcaga
     1201 tgggctacct gcctggcaca ctgtggctgc tggcgggggt agtgctggcc ggtgcggtac
     1261 aggactttat ggtgctgttt atctcctctc gccgtaacgg cgcatctctc ggtgagatga
     1321 tcaaagaaga gatgggacca gtaccgggga ctatcgcgct gtttggctgt ttcttaatca
     1381 tgatcatcat ccttgccgtc ctggcgctga ttgtggttaa agccctggcc gaaagtccgt
     1441 ggggtgtctt caccgtttgc tcaaccgtac caattgcgct gtttatgggt atctacatgc
     1501 gctttatccg tccggggcgt gtgggtgaag tctctgtcat tggtatcgtg ctgctggttg
     1561 cctctatcta cttcggtggc gtgattgctc acgatccgta ctggggtccg gcactgacct
     1621 ttaaagacac caccattacc ttcgcgctga ttggctatgc gtttgtttcc gcgctgctgc
     1681 cggtgtggct gatcctcgca ccacgcgact atctggcaac cttcctgaaa atcggcgtta
     1741 tcgtcggcct ggcgctgggt atcgtggtgc tgaacccgga actgaaaatg cctgcgatga
     1801 cccagtacat tgacggtact ggcccgctgt ggaaaggcgc tctgttcccg ttcctgttca
     1861 tcaccatcgc ctgtggtgcg gtatctggct tccacgcact gatctcttcc ggtactacgc
     1921 cgaaactgct ggctaatgaa accgacgcgc gtttcatcgg ttatggcgca atgctgatgg
     1981 agtccttcgt ggcgattatg gcgctggttg cagcgtccat catcgaaccg ggtctttact
     2041 tcgcgatgaa caccccgcct gctggccttg gcatcaccat gcctaacctg catgaaatgg
     2101 gtggcgagaa cgcgccgatc atcatggcgc agttaaaaga cgttaccgca cacgcggcgg
     2161 cgaccgtcag ctcctggggc ttcgtgattt cgccagagca gatcctgcaa accgcgaaag
     2221 acattggtga gccttctgtc ctgaaccgtg caggtggtgc gccgacgctg gcggtaggta
     2281 tcgctcacgt gttccacaaa gtgctgccga tggctgacat gggcttctgg tatcacttcg
     2341 gtattctgtt cgaagccctg ttcatcctga ccgcgctgga tgcgggtacc cgttctggcc
     2401 gctttatgct gcaagatctg ctgggtaact tcatcccgtt cctgaaaaaa accgactctc
     2461 tggttgccgg tatcatcggt actgcgggct gtgtgggtct gtggggctac ctgctgtatc
     2521 agggcgtggt cgatccgctg ggcggcgtta agagcctgtg gccgctgttc ggtatttcca
     2581 accagatgct ggcagccgta gcgctggtac tgggcaccgt tgtgctgatt aagatgaagc
     2641 gcacccaata catctgggta actgttgttc cggctgtatg gctgcttatc tgcaccacct
     2701 gggcgctggg tctgaaactg ttcagtacca acccgcagat ggaaggcttc ttctacatgg
     2761 caagccagta caaagagaag attgctaacg gtactgacct gacggcgcag cagattgcca
     2821 acatgaacca catcgttgtg aacaactaca ccaacgcagg cctgagtatt ctgttcctga
     2881 ttgtggtgta cagcatcatc ttctacggtt tcaaaacctg gcttgcggtg cgtaacagcg
     2941 acaaacgtac tgacaaagaa acaccgtacg ttccaatccc ggaaggcggc gtgaagatct
     3001 cttcgcacca ctaaccgtgt ttagccccgc ttcggcgggg ctttgttctt atcagagtga
     3061 actatgtttg gtaacttagg acaggcaaaa aaatatctcg gccaggcggc aaagatgttg
     3121 attggcattc cagactacga caactacgtc gagcatatga agaccaacca tcccgacaag
     3181 ccgtacatga gctatgaaga attcttccgc gaacgccaga acgcgcgcta cggcggcgat
     3241 ggtaaaggcg gtatgcgctg ttgttaatgg agagagcatg aacccgattg cagttaccct
     3301 actcaccggt tttttaggcg cgggaaaaac caccctgctg cgccatattc ttaacgaaca
     3361 acacggctac aagattgccg tgattgaaaa cgaattcggc gaagtctccg ttgatgatca
     3421 actgattggc gaccgcgcca cgcagatcaa aacgctgacc aacggctgca tctgctgttc
     3481 gcgctccaac gagctggaag acgcactact cgacctgctg gataatctcg acaagggcaa
     3541 tattcagttc gaccgtctgg tcattgaatg caccggcatg gccgatcccg gcccgattat
     3601 tcagaccttt ttctcccatg agattttatg ccagcgttac ctgctggacg gcgtgattgc
     3661 gctggtggat gcggtacatg ccgatgagca gatgaaccag ttcaccatcg cccagtccca
     3721 ggtgggctac gccgaccgta ttctgatgac caaaaccgac gtcgcaggcg aagcagaaaa
     3781 actgcgtgaa cgcctggcgc gcatcaacgc ccgtgcaccg gtctacaccg ttacccacgg
     3841 cgacatcgac ctgggcctgc tgttcaacac caacggtttt atgctggaag aaaacgtcgt
     3901 cagcaccaaa ccgcgtttcc actttatcgc ggataaacaa aacgatattt cgtcgattgt
     3961 ggtagaactg gattacccgg tagatatcag cgaagtttcc cgggtgatgg aaaacctgct
     4021 gctggaatcg gcggataaac tactgcgtta caaagggatg ctatggattg acggcgaacc
     4081 taaccgcctg ctgttccagg gcgtccagcg cctctacagc gccgactggg accgcccgtg
     4141 gggcgatgaa aaaccgcata gcacgatggt gtttatcggg attcagctgc cggaggaaga
     4201 gattcgggcg gcgtttgcgg ggttgaggaa gtaaggggtt atgggccgga taaggcgcag
     4261 ccgcatccgg cctgatattt cactcaaaat agtccatatc cagtttcggc accttcaaca
     4321 aacgtgaaga aacccctact tcgttttcga tcattaagtg caccaggcgt tccccatcaa
     4381 ccaacaccat accctcgacg gattgagcaa agtcacgcgc ctgagacgta aatccagaag
     4441 tggtaataaa caccccacgt ttcgcctttt gcccagccag tgcgccgtaa aatgcctgta
     4501 attctggcct gcctacagtg ttctgccaac gttttgcctg aacataaact ttctccaggc
     4561 caagtttatc aagcgatatc acaccatcga tgccaccatc tccagtaccg ccaacacgct
     4621 gcaaatcatc acggtggccg ccatacccca ggcgatgcaa aacatccaga acaatgactt
     4681 caaagcgcga aggagaaacc tgcaataagt tttccagaac ctcatcagcc accgcatcac
     4741 gaagctcttt tagcgcctga tctaaccgat cgtccgggct gctctttgca agttcttcat
     4801 gatcgggaga gtcggctttc ggatctaaat cgacggcatc cggccgtgac ttaagtttga
     4861 cattcacaaa atcgaaggcc agatggttcg tctcctgctc cgtcattggc tggggatgag
     4921 acgcaaccca gtcaaaaccc gcaggagtca ggcaccattt gccacgcgac aaactttgcg
     4981 acaacccggc acgttttaaa cggtcatgcg cccagcctgc acgattttta taaacaagtt
     5041 gtccgctggt aatgactttc gctcgctggc tgtcatccag tcctaatgca tccgcggcag
     5101 cctcatgaac atcacgcgcg gctgcacctt ccggttttgt cgccagataa cgcagaacag
     5161 gttcaataaa tttgtcatag gtaggaaccg tcatagtaca tccttgcaga atcaggtaga
     5221 tgtttttcgg ctactataac actaccaaaa tagacgaaca cgttagaaat gagtcagttg
     5281 ctgtgaccgt ggtcattgcc cggaaaggta cagaaagcta agataaggcg ttatgggcct
     5341 taaatatttg gacaggccca cacagcaatg gattaataac aatgatgaat aaatccaatt
     5401 ttgaattcct gaagggcgtc aacgacttta cctatgccat cgcctgtgcg gcggaaaata
     5461 actacccgga tgatcccaac acgacgctga ttaaaatgcg tatgtttggc gaagccacag
     5521 cgaaacatct tggtctgtta ctcaacatcc ccccttgtga gaatcaacac gatctcctgc
     5581 gcgaactcgg caaaatcgcc ttcgttgatg acaacattct ctctgtattc cacaaattac
     5641 gccgcattgg taaccaggcg gtgcacgaat atcataacga tctcgacgat gcccagatgt
     5701 gcctgcgact cggcttccgc ctggctgtct ggtactaccg tctggtcacc aaagattatg
     5761 acttcccggt accggtgttt gtgttgccgg aacgtggcga aaacctctat caccaggaag
     5821 tgctgacgct aaaacaacag cttgaacagc aggtgcgaga aaaagcgcag agtcaggcag
     5881 aagtcgaagc acaacagcag aagctggttg ccctgaacgg ctatatcgcc attctggaag
     5941 gcaagcagca ggaaaccgaa gcgcaaaccc aggctcgcct tgctgcactg gaagcacagc
     6001 tcgccgagaa gaacgcggaa ctggccaagc agaccgaaca ggaacgtaag gcttaccaca
     6061 aagaaattac cgatcaggcc atcaagcgta cactcaatct tagcgaagaa gagagccgct
     6121 tcctgattga cgcgcaactg cgtaaagcag gctggcaggc cgatagcaaa accctgcgct
     6181 tctccaaagg cgcgcgcccg gaacccggcg tcaataaagc cattgccgaa tggccgaccg
     6241 ggaaagatga aacgggtaat cagggctttg cggattatgt gctgtttgtc ggtctcaaac
     6301 ccatcgcggt ggtagaagcg aaacgtaaaa atatcgacgt tcccggcaag ctcaatgagt
     6361 cgtatcgcta cagtaaatgt ttcgataatg gcttcctgcg ggaaaccttg cttgagcact
     6421 actcaccgga tgaagtgcat gaagcggtgc cggagtatga aactagttgg caggacacca
     6481 gcggccaaca acggtttaaa atccccttct gctactcgac caacgggcgc gaataccgcg
     6541 cagcgatgaa gactaaaagt ggcatctggt atcgcgacgt gcgtgatacc cgcaatatgt
     6601 cgaaagcctt acccgagtgg catcgcccgg aagagttgct ggaaatgctc ggcagcgaac
     6661 cgcaaaaaca gaatcagtgg tttgccgata atcccggcat gagcgagctg ggcctgcgtt
     6721 attatcagga agatgccgtc cgcgcggttg aaaaggcaat cgtcaagggt caacaagaga
     6781 tcctgctggc gatggcgacc ggtaccggta aaacccgcac cgcaattgcc atgatgttcc
     6841 gcctgatcca gtcccagcgt tttaaacgca ttctcttcct tgtggatcgc cgttctcttg
     6901 gcgagcaggc gctgggggcg tttgaagata cgcgtattaa cggcgacact ttcaacagca
     6961 ttttcgacat taaagggctg acggataaat tccccgaaga cagcaccaaa attcacgttg
     7021 ccaccgtaca gtcgctggtg aaacgcaccc tgcaatcgga tgaaccgatg ccggtagccc
     7081 gttacgactg tatcgtcgtc gatgaagcgc atcgcggcta tattctcgat aaagagcaga
     7141 ccgaaggcga actgcagttc cgcagccaac tggattacgt ttctgcctac cgtcgtattc
     7201 tcgatcactt cgatgcggta aaaattgctc tcaccgccac cccggcgctg catacggtgc
     7261 agattttcgg cgagccggtt taccgttata cctaccgtac cgcggttatc gacggttttc
     7321 tgatcgacca agatccgccg attcaaatca ccacccgcaa cgcccaggaa ggggtttatc
     7381 tctccaaagg cgagcaggtt gagcgcatca gcccgcaggg ggaagtgatc aacgacaccc
     7441 tggaagacga tcaggatttt gaggtcgccg actttaaccg tggcctggtg atcccggcgt
     7501 ttaaccgcgc cgtctgtaac gaactcacca attaccttga cccgaccggg tcacaaaaaa
     7561 cgctggtctt ctgcgtcact aatgctcatg ccgatatggt ggtggaagag ctgcgtaccg
     7621 cattcaagaa aaagtatccg caactggagc acgacgcgat catcaagatc actggtgatg
     7681 ccgataaaga cgcgcgcaaa gtgcagacca tgatcacccg cttcaataaa gagcggctgc
     7741 ccaatattgt ggtaaccgtc gacctgctga cgaccggcgt cgatattccg tcgatctgta
     7801 acatcgtgtt cctgcgtaaa gtacgcagcc gtattctgta cgagcaaatg aaaggccgcg
     7861 ccacgcgctt atgcccggac gtgaataaaa ccagctttaa gattttcgac tgcgtcgata
     7921 tctacagcac gctggagagc gtcgacacca tgcgtccggt ggtggttcgc ccgaaggtgg
     7981 aactgcaaac gctggtcaac gaaattaccg attcagaaac ctataaaatc accgaagcgg
     8041 acggccgtag ctttgccgag cacagccatg aacaactggt ggcgaagctc cagcgcatca
     8101 tcggtctggc cacgtttaac cgtgaccgca gcgaaacgat agataaacag gtgcgtcgtc
     8161 tggatgagct atgccaggac gcggcgggcg tgaactttaa cggcttcgcc tcgcgcctgc
     8221 gggaaaaagg gccgcactgg agcgccgaag tctttaacaa actgcctggc tttatcgccc
     8281 gtctggaaaa gctgaaaacc gacatcaaca acctgaatga tgcgccgatc ttcctcgata
     8341 tcgacgatga agtggtgagt gtaaaatcgc tgtacggtga ttacgacacg ccgcaggatt
     8401 tcctcgaagc ctttgactcg ctggtgcaac gttccccgaa cgcgcaacca gcattgcagg
     8461 cggtgattaa tcgcccgcgc gatctcaccc gtaaagggct ggtcgagcta caggagtggt
     8521 ttgaccgcca gcactttgag gaatcttccc tgcgcaaagc gtggaaagag acgcgcaatg
     8581 aagatatcgc cgcccggctg attggacata ttcgccgcgc tgcggtgggc gatgcgctga
     8641 aaccgtttga ggaacgtgtc gatcacgcgc tgacgcgcat taagggcgaa aacgactgga
     8701 gcagcgagca attaagctgg ctcgatcgtt tagcgcaggc gctgaaagag aaagtggtgc
     8761 tcgacgacga tgtcttcaaa accggcaact tccaccgtcg cggcgggaag gcgatgctgc
     8821 aaagaacctt tgacgataat ctcgacagcc tgctcgataa attcagcgat tatatctggg
     8881 acgagctggc ctgacacggt atcattcccg gcaatttctt cttctgcggc ccaataaatt
     8941 tgggccgctg cttaatttac ggaacaccca atgaacaata acgatctggt cgcgaagctg
     9001 tggaaactgt gcgacaacct gcgcgatggc ggcgtttcct atcaaaacta cgtcaatgaa
     9061 ctcgcctcgc tgctgttttt gaaaatgtgt aaagagaccg gacaggaagc ggagtacctg
     9121 ccggtaggct accgctggga tgacctgaaa tcccgcatcg gccaggagca gttgcagttc
     9181 taccgtaacc tgctggtgca tctgggcgcc gacaatcaaa agctggtgca ggcggtgttc
     9241 cagaacgtca acaccaccat tacccagcca aaacagttga ccgaactggt cagcaatatg
     9301 gattcactgg actggtacag cggcgcgcac ggtaagtcgc gcgatgactt cggcgatatg
     9361 tacgaaggtc tgttgcagaa gaacgcgaac gaaaccaagt ctggcgcggg ccagtacttc
     9421 accccgcgtc cgctgataaa aaccattatt catctgctga aaccacagcc gcgtgaagtg
     9481 gtacaggacc cggcagcagg tacggcgggc tttttgattg aagccgaccg ctacgttaag
     9541 tcgcaaacca atgatctgga cgaccttgat ggtgacacgc aggatttcca gatccaccgc
     9601 gcgtttatcg gcctcgaact ggtgcctggc acccgtcgtc tggcgctgat gaactgcctg
     9661 ctgcacgata ttgaaggcaa cctcgaccac ggcggcgcaa tccgtctggg caacaccctg
     9721 ggtagcgacg gcgaaaacct gccgaaggcg catattgtcg ccactaaccc gccgtttggc
     9781 agcgccgcag gcaccaacat tacccgcacc tttgttcacc cgaccagcaa caaacagttg
     9841 tgctttatgc agcatattat cgaaacgctg caccccggcg gtcgtgcggc ggtggtggtg
     9901 ccggataacg tgctgtttga aggcggcaaa ggcaccgaca tccgtcgtga cctgatggat
     9961 aagtgtcatc tgcacactat tctgcgtctg ccgaccggta ttttttacgc tcagggcgtg
    10021 aagaccaacg tgctgttctt taccaaaggg acggtggcga acccgaatca ggataagaac
    10081 tgtaccgatg atgtgtgggt gtacgacctg cgtaccaata tgccgagctt tggtaaacgc
    10141 acgccgttta ccgacgagca tttgcagccg tttgaacggg tgtatggcga agacccgcac
    10201 ggcttaagcc cccgtactga aggtgaatgg agttttaacg ctgaagagac ggaagttgcc
    10261 gacagcgaag agaacaaaaa caccgaccag cacctggcca ccagccgctg gcgcaagttc
    10321 agccgtgagt ggatccgcac cgcgaaatcc gattcgctgg atatctcctg gctgaaagat
    10381 aaagacagca ttgatgccga caacctgccg gagccggatg tattagcggc agaagcgatg
    10441 ggcgaactgg tacaggcact gggtgaactg gatgcgctga tgcgtgaact gggggcgagc
    10501 gatgaggcgg atgcgcagcg tcagttgctg gaagaagcgt ttggtggggt gaaggaatga
    10561 gtttaaaaaa tattccaacg aattggtgtg taactaccat aggggatatt aatgactata
    10621 aatcattaac ttttaatccc aaatcatacc aggaggacag cttcgaattg tatagcgttc
    10681 ccagctttgc atctggctca ccagaaatat tactgggaaa agatattgcc tccaataaac
    10741 agtatattca gtttaaagat attttattat gcaaaattaa tccccgtatt aatcgcgttt
    10801 ggtgtgtaaa agaaaaaaaa gaacatcctc aaatcggttc atctgaatgg attgtaatca
    10861 ggcaaccgct cattcatgaa ggttttttgg cctatttttt gcaaacacaa gatttcagag
    10921 atctcctatg ttctgatgtt tctggtgtgg gtggttctct gacgagagcg caacccaaaa
    10981 gagtcgctac atataatgtc cctatagcac ctttgccaga acaaaaaatc atcgctgaaa
    11041 aactcgatac gctgctggcg caggtagaga gcaccaaagc acgtcttgag caaatcccac
    11101 aaatcctgaa acgttttcgt caggcggtgc tgggtgctgc tgtcagcgga aaacttacac
    11161 aaaattggag agaaaacaat
//
LOCUS       DAZFKF010000138        11134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFKF010000138 DAZFKF010000000
VERSION     DAZFKF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.140"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            129..3419
                     /gene="yjiT"
                     /locus_tag="ACHKA1_004614"
     CDS             129..3419
                     /gene="yjiT"
                     /locus_tag="ACHKA1_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048814575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjiT"
                     /protein_id="HFO5682169.1"
                     /translation="MGQSEYISWVKCTSWLSNFVNLRGLRKPDGRPLYEYHATNDEYN
                     QLTQLLRAVGQSQSNICNKDFAACFVLFCSEWYRRDYERQCGWTWDPIYKKIGISFTA
                     TELGTIVPKGMDDYWLRPIRFYESERRNFLGTLFSEGGLPFRLLKESDSRFLAVFSRI
                     LGQYEQAKQSGFSALSLARAVIEKSALPTVFSEDTSVELISHMADNLNSLVLTHNLIN
                     HKEPVQQLDKVHPTWRSEFPIPLDDETGTHFLNGLLCAASVEAKPRLQKNKSTRCQFY
                     WSEKHPDELRAIVSLPDEVSFPVTSEPSTTRFELAICEDGEEVSGLGPAYASLENRQA
                     TVRLRKSEVRFVRQNPSAGLSLVARAGGMIVGSIKLDDSEIAIGEVPLTFIVDADQWL
                     LQGQASCSVRSSDVLIVLPRDNSTVAGFDGQSRAVNVLGLKALPVKGCQDVTVTANET
                     YRIRTGREQISIGRFALNGKRASWVCHPDETFIGVPKVISTLPDIQSIDVTRYLSGIS
                     IEQCHIQEMLGAQYLSIRNSNNETLLRRKIGILPADFSIEIKGGMHANEGTIVITTQH
                     PCVATLKDKTLEATRKRTEGRTEIQLKAEGVPPAFITLQVLPNLAADTIDIELPFPAK
                     GCLALDVNGRPLDKNITLHDLLGSRAFLFSRNGEPTKYTLQLHLRSISGLQAWHEWSY
                     TALSERPVELNLYSLREHIENLISLEAGIDQVVEMRITGAGVVMAWQIRRYKYSLRYD
                     YEKELLLSQSVNHRAGQIPSPVIMLLSEPERKSIPLVSRMSEGVPVGEYELSSIVNKN
                     GPWLVVPKPGEEMAFRPCFIRGESSLPVEESNIRSLQKATQLFNPQAEVNAITLVLGQ
                     MANDPAHSGWQFMRSLYDQFGYLPLATFEVWRALVQHPQALAMSLFKFEMSAEYLSRI
                     ENEFPILWEFFPIFEIKAASERFKLFLSQKGAPEETQKLLVTNMFQRLGLVFPTYADE
                     IEKWLSNGYLPPSIPESCVHGWYQELLREHSEARWPEYGCKRLYKWMMSQKNPVIDIN
                     PDANHRYSVAWLPVFAAAVASGNASFESVFDRKPGAVFFLRQVRDFDSRWFKAIFQCS
                     LLRYVAKK"
     gene            3432..9776
                     /locus_tag="ACHKA1_004615"
     CDS             3432..9776
                     /locus_tag="ACHKA1_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO5682170.1"
                     /translation="MTTRYFSSLIEQSLSRSTEATLSIMGVTNPKLREHLAQQMGADC
                     GKPGSFLASPVFQQMFGWKESNHTMRSLTEGNALLSKAVVDSLDDQSNGRYRFGADWK
                     PFTHQLASWKALLEKKHSVVVTSGTGSGKTECFMVPVLEDLYRELHENGNNPLVGVRA
                     LFLYPLNALINSQRERLDAWTRGFGTGIRYCLYNGNTENLHASVKSEQAKRPNEVLSR
                     EKMREEPAPILVTNGTMLEYMMVRQVDAPIIQQSKAQKSLRWIVLDEAHTYVGSQAAE
                     LALQLRRVMTAFGVTPDDVRFVATSATIAGSDAEKQLKKFLSELSGIAQERIDVLDGS
                     RVIPELEPCKNVSVPLDEIEQIPDLDLKDKNDNKIKGISPERFYALTHSPEARYIREM
                     LVSQPNPMKLDEMTQRLNALTKQHYSQQEVLRWIDVCSGTQPNAKDPAFLKVRAHIFQ
                     RNTQGIWACVDKNCRLKHGTPLEKGWPFGYVYVNQRQNCDCGSPVYEVAFCNDCNEPH
                     LLAQDKKGKLVQWENKGGDEFSLQDEVPVEHDATEEKVEKENSFQPPLIIAAGETSEA
                     GYTLQRLDRQTRRIGVINNDSIPLIINDVEQVCSASGCGYRGMSGKQPFRRALLGGPF
                     YVTNIVPTVLEYCQDFTSDEGKEGIGPDSLPGRGRRLITFTDSRQGTARMAVRMQQEA
                     ERSLLRGSVVEILSWHQRTQTSTAPNANADLEKLAARAKQAREQAEEYRSWGMPDQAK
                     LSQAQAEQLEQAYQAATGGKAATTLVSRTWTEMVNELKDKADIRGPVLKYNHYLQPEV
                     FNKNGGPLKLSEMLLFREFMRRPKRTNSLETQGLVQVGYQGLEKIHKSPLHWQEKGLT
                     LDDWRDFLKVTLDLYVRESNFTQLDDELKNWIGSRFSSKFVRNPESKDPEDNQNRRWP
                     QIRNGNVSHRLAKLLMLGAGFKTVNTATIDIINTWLKEAWAQLTGPLAVLKPDGNRFY
                     LPKEHMTFSLITDAWICPVTNKILDTAFKGLTPYLPTHISFEHLTQAQYDTFVAQKVT
                     MPEIWKLDRSQEDYAEGLAKARDWVNNDPLIAQLRSENVWTDINDRVVEGGFYYRTAE
                     HSAQQSSERLQSYEKMFKNGQLNVLNCSTTMEMGVDIGGITAVVMNNVPPHPANYLQR
                     AGRAGRSKESRAISYTLCKGNPHDQQVFANPLWPFETMIPAPMVAMNSARLVQRHVNA
                     LLLSDFLCNVIGETDKEKTSLDSLWFFGEDDGQSKCERFKVWLERPVLDIDTALERLV
                     KGTALHGARAEHLRDKTINAITFLQQRWLSVYRDLVTQERESQPQTPYRKRIELEKKR
                     HCGEYLLRDLAARTFLPGYGFPTDVVTFDNFTMEDYIREKSQKSRDKKDREDNVSRYK
                     GLPSRNLGVAIREYAPGAEIILDGRVFRSAGVSLHWHNINADTNEAQRLDCAWRCHKC
                     GTIGYEEGMSSSGMLFCSNSACGEKITMDNRRQVLQPAGFVTDAHAPVTNNIETMKFV
                     PVVPAWVFVKAEPVPLPNPLMGYMASGADGHVFQQSLGEGGHGYALCLSCGRAESMLN
                     ENDAPKSMEAHYPPRPGKADRDSQNHRLICPGSTALMKNVTLGALARTDVFEMVLRKP
                     QNGEYLPDNTEEGRIVAMTLAVALRQALAGVLGISAAELGYSVRPVRLEDGQSVLAVQ
                     LYDVISGGAGFASSAPVHIEAILQGMVKQLGCRHCDTACSECLLDSQTRHDHDLLDRK
                     VALAWLGDDFTYYIGLPDEETFSLPDARYCPGAIGDTIRRAINEGAEKLTLWMTGAPN
                     EWDLYARQFRAAIQSYRLKDNVEVDLVIPAGVDDPDLLHELSQFTALGVRLCHVEQEL
                     QLPIVAQVTFADRVMTLASRSQQATIPGPEWHLNDELVVRSLGYQTVELNEFILPAKA
                     ANAVERVKDIQIHKQLNGPLSQFGQRFWDVLFNDHEEAQSLMKNTRITGVHYTDRYLQ
                     NPVALALLGSILKPLKTKLTDGAEVALDTLFKDKDRPGNRPFHDWMSIADFQDFADQW
                     FAAALGRPIELTVFDSPRDIPHHRKLTVTFEDGQVLKIRFDQGMGYWRINFASQWHYF
                     DFRDDVSFQLVKMAQACKEGNVANSEESWATDVLVEVTAS"
     gene            complement(9819..10160)
                     /gene="symE"
                     /locus_tag="ACHKA1_004616"
     CDS             complement(9819..10160)
                     /gene="symE"
                     /locus_tag="ACHKA1_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO5682171.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPVAEESELMQSLRQVCKLSARKQKQVQDF
                     IGVIAGKQKVA"
     gene            complement(10388..>11134)
                     /locus_tag="ACHKA1_004617"
     CDS             complement(10388..>11134)
                     /locus_tag="ACHKA1_004617"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010671752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO5682172.1"
                     /translation="PADCWEWVCFEQICETDNNALKAGPFGSALKKSDCVKSGHKVYG
                     QEQVISGQESLETYRITEDKYQQLKSCATKPGDILISLVGTIGKTLILSNLAETGIIN
                     PRLVKIALHSEIQRQYVKLYLDGPLAYDFFKGFSHGGTMDILNLGILKELPIPLPSLE
                     EQREIVRRVEQLFAYADTIEKQVNNALARVNNLTQSILAKAFRGELTAQWRAENPELI
                     SGENSAAALLEKIKAERAASGGKKASRKKS"
BASE COUNT         2896 a         2556 c         2858 g         2824 t
ORIGIN      
        1 tatggtgccg acctgaaaaa ttagctattg ccgcctgatt aattatacga tatttttaaa
       61 ggttttcttt caaattagaa atattctggg gttttgtttt tgtatctttt atctctaagg
      121 agcttgcttt gggtcaatca gaatacattt catgggtaaa atgtacttcc tggctaagta
      181 actttgtgaa tcttagaggg ttgagaaaac cggatggccg tcctctttat gaatatcatg
      241 caaccaatga tgaatataac caattaacgc agctactccg tgcagtcggt caatcacaat
      301 ctaatatatg taataaggac tttgctgcct gttttgttct tttttgctct gagtggtacc
      361 gcagggatta tgaacggcaa tgcggatgga cgtgggatcc tatatataaa aaaataggga
      421 tttcatttac cgccacagag cttggtacta tcgtaccgaa agggatggac gattactggc
      481 tgcgcccaat tcgattttat gaatctgaac gacgcaattt tctcggaaca ttattcagtg
      541 aaggtggttt acctttccgg ttactcaaag aatcagacag tcgctttctg gctgttttct
      601 ccaggatcct tggacaatat gagcaagcaa agcagtcagg tttttctgcc ctttcgctgg
      661 caagagccgt catagaaaaa tcagcgctgc caactgtttt tagcgaagat acctctgtag
      721 aacttatcag tcatatggct gataacctca attccttagt acttactcac aacctaataa
      781 atcacaaaga accagtacaa cagcttgata aggttcaccc tacctggcga agcgagtttc
      841 caataccgtt agatgatgaa acaggaacgc attttttgaa tggactgcta tgcgcagcct
      901 ctgtagaggc taagcctcga ctacaaaaaa ataagagtac gcgttgccaa ttctattggt
      961 cagaaaagca tccagatgag cttagggcga tagtatctct ccctgacgaa gtttcctttc
     1021 ctgtaacaag cgagccgtca actacgcgct ttgaacttgc catttgtgaa gatggtgagg
     1081 aagtctctgg ccttgggcca gcctatgctt ccctggaaaa cagacaggca acagttcgat
     1141 tacgtaaaag cgaagtgaga tttgtcaggc aaaatccatc ggcaggtttg tcgttagtgg
     1201 ctcgtgctgg agggatgatt gttgggagca ttaaacttga tgacagtgaa attgccattg
     1261 gtgaggtgcc gttaaccttc atcgttgatg cagatcaatg gctgttacag ggacaggcct
     1321 cctgcagtgt gcgaagcagc gatgttctga ttgtgctccc tcgggacaat agcactgttg
     1381 ctggttttga tggccaatcg agagcagtaa acgtattagg acttaaagcg ctacctgtta
     1441 agggatgtca ggacgttaca gttacagcaa atgaaactta tcggattcgc accggaagag
     1501 aacaaatcag catcggaagg tttgctctaa atggaaagcg tgcaagctgg gtttgtcatc
     1561 cagatgagac atttattggt gtaccaaaag tcatttctac actaccagat attcaaagca
     1621 ttgatgtaac gcgttacctg agcggaattt caattgagca atgtcatata caagagatgc
     1681 taggggctca atatctttct atccgtaata gtaataatga aacgctcttg cgtcgcaaaa
     1741 tcggtatctt gcctgctgat ttcagtattg agataaaagg tggaatgcac gcaaatgaag
     1801 gcactatcgt aatcaccaca cagcatcctt gtgtagcaac acttaaagat aaaacattgg
     1861 aggctacacg taagcgtact gaggggcgaa cagagataca actcaaagcg gaaggggtac
     1921 ctccggcatt tattacatta caagttctgc ctaatttggc ggctgatacc atcgacattg
     1981 agttaccttt cccagctaaa ggctgccttg ctcttgatgt aaacggacgt cctctggata
     2041 aaaacattac gctgcatgat ctgctgggtt ccagagcatt cctgttctcc agaaatggtg
     2101 agccgacaaa atatacattg cagttgcatt tgcgctccat tagtggatta caggcatggc
     2161 acgaatggag ttatacggca ttgagcgagc gtcctgttga attgaatctc tatagtctgc
     2221 gcgaacatat tgagaacctg atttcacttg aggcaggtat tgatcaggtt gttgagatgc
     2281 gtattacggg ggcgggtgtg gtcatggcct ggcaaattcg tcgctataaa tacagcctgc
     2341 gctatgatta tgaaaaagaa ctattactca gccagtcagt gaaccatcgt gctgggcaga
     2401 ttccttcccc cgttatcatg ctgttaagtg aacccgagag gaaatccatt cccttagtat
     2461 ctagaatgag cgagggagtg cctgtcggtg aatatgagtt gagctcaatc gttaacaaaa
     2521 atggaccatg gttggttgtt cctaaaccgg gtgaagagat ggcttttcgt ccctgcttca
     2581 ttcgtgggga atcttcgtta ccggtagaag aaagcaacat ccgctcattg cagaaagcga
     2641 cccagctttt taatccacag gcagaagtta atgccatcac cctggtgctc ggacagatgg
     2701 ctaacgatcc tgcccattcc ggctggcaat ttatgcgtag cctgtacgat caatttggtt
     2761 atttaccgct ggcaaccttt gaagtatggc gtgcactggt gcagcatccg caggcattgg
     2821 caatgtcgct cttcaaattt gaaatgtcag cagagtacct gagtcgtatt gagaatgagt
     2881 tccctatttt atgggagttt ttccccatct ttgagatcaa agctgcatct gagcgtttta
     2941 aattgttcct gagccaaaaa ggtgcaccgg aggaaactca gaagttactg gttactaata
     3001 tgtttcaacg gctggggctc gtgttcccga catatgccga tgaaattgag aaatggttaa
     3061 gtaatggata tctccccccg tctattcctg aatcttgtgt ccacgggtgg tatcaggagc
     3121 tcttacgtga gcacagtgag gctcgctggc ctgaatatgg ctgcaagcgt ctgtataaat
     3181 ggatgatgtc acaaaaaaat ccagtgattg atattaatcc tgacgcaaat caccgctatt
     3241 ccgtggcctg gttacccgtc ttcgccgcag cggtggcaag cggaaatgct tcttttgaat
     3301 ctgtatttga ccgtaaaccc ggtgccgtat ttttcctgag gcaagtcagg gatttcgatt
     3361 cccgctggtt taaggccatc ttccagtgca gcctgttgcg ttatgttgct aaaaaataaa
     3421 aaggaataag gatgactaca cgctattttt cgtcacttat tgaacaatcg ttgtctcgtt
     3481 caactgaagc cacgctcagt atcatgggcg ttacaaaccc gaagttgcgt gaacatctcg
     3541 cgcagcaaat gggggctgat tgtgggaaac cgggctcatt ccttgcttct ccggtattcc
     3601 agcaaatgtt tggctggaag gagtcaaacc acacgatgcg cagcctgacg gaaggcaacg
     3661 cattgttgag caaagcagtg gttgattcac tggatgatca gagtaatggc cggtaccggt
     3721 ttggtgctga ctggaagcct tttacgcacc agttagccag ttggaaagct ttgctggaaa
     3781 agaaacattc ggtggtggta accagtggta cgggttcagg taaaacggaa tgttttatgg
     3841 ttccggtgct ggaagatctt taccgggaac tgcatgaaaa tggcaacaat ccgttggtcg
     3901 gtgtccgagc cctttttctc tatccattga acgccctgat taattcacaa cgcgagcgtc
     3961 tggatgcctg gacgcgtggt tttggtaccg ggatccgcta ctgcctttac aacggcaaca
     4021 ccgaaaacct acacgcttcg gtaaaaagcg aacaggcaaa aaggccgaat gaggtgctct
     4081 cgcgcgaaaa aatgcgtgaa gaacctgcgc cgattctggt gaccaacggc acgatgctgg
     4141 aatatatgat ggttcgccag gtcgacgccc cgattatcca gcaatccaaa gcacaaaaat
     4201 cactacgctg gattgtactg gatgaagcgc atacctatgt cgggtcgcag gccgctgaac
     4261 tggcgctcca gctgcgtcgt gtgatgacag cgtttggcgt gacgccagat gacgtgcgct
     4321 ttgtggcgac ctctgcgacc attgccggca gcgacgctga aaagcagctg aaaaaattcc
     4381 tctctgaatt atccggcatt gcgcaggagc gcatcgatgt tctggacgga agtcgggtga
     4441 ttcctgaact ggaaccatgc aaaaacgttt ctgttccgtt agacgaaata gagcaaatac
     4501 ctgatctgga tctaaaagat aaaaatgata ataaaattaa agggataagt cctgagcgct
     4561 tttatgcgct tacgcactcc cccgaagcgc gttacatacg tgaaatgttg gttagccaac
     4621 ccaatccaat gaaactggat gagatgacgc agcggttaaa tgcgctgaca aaacagcatt
     4681 acagccagca ggaagtactg cgttggatcg atgtctgttc agggactcaa cctaatgcaa
     4741 aggatcccgc atttcttaaa gtcagggcgc atatcttcca gcgtaatacc cagggaatat
     4801 gggcttgtgt ggataaaaat tgcagattaa agcacggtac accgctcgaa aaaggctggc
     4861 cctttggcta tgtgtatgtg aaccagcgac aaaattgcga ctgtggaagc cctgtctacg
     4921 aagtggcgtt ctgtaatgat tgtaatgagc ctcatcttct ggcgcaggac aaaaagggca
     4981 aacttgtcca atgggaaaat aaaggtggcg atgagttctc cttgcaggat gaagtacctg
     5041 ttgaacatga cgctacagaa gaaaaagtcg aaaaagagaa cagtttccag cctccgttga
     5101 ttattgccgc aggagagacc agcgaggccg gttataccct acaacgcctc gaccgccaga
     5161 cgcgccgtat tggcgttatt aacaatgaca gcattccgct gattattaat gatgttgaac
     5221 aggtttgtag cgccagtggc tgtggctaca gaggcatgag tgggaaacag cccttccggc
     5281 gtgcactatt aggtgggcca ttttacgtta ctaatatcgt gcccaccgtt cttgagtatt
     5341 gtcaggactt taccagtgat gaaggcaaag agggcatcgg accagattcg ttgccagggc
     5401 gaggacgtcg actcattacc tttacggaca gtcgacaagg tacagctcga atggcggtgc
     5461 gtatgcagca agaagcagaa cgcagtctct tacgcggaag cgtagtcgaa attctgagct
     5521 ggcatcaacg gacgcaaacg tccacggcgc cgaatgccaa tgccgatctg gaaaaattag
     5581 cggccagggc gaagcaagcc cgtgagcagg cagaagaata tcgaagctgg ggaatgccag
     5641 accaggcgaa attgtcacaa gcgcaggctg aacagcttga acaggcttat caggctgcaa
     5701 ccggtggaaa agcagcaact accctggtat cccgcacctg gacagaaatg gttaacgagc
     5761 ttaaagataa agccgatatc cgtggcccgg tactgaaata taaccactat ttgcagcctg
     5821 aagtgtttaa taaaaacggc gggccactaa agctttcgga aatgttgttg ttccgggaat
     5881 tcatgcgtcg ccctaaacgg actaacagcc tggaaacaca ggggctggtt caggttggtt
     5941 accaggggct ggagaaaata cataagagcc ccttgcactg gcaggaaaaa ggattaacgc
     6001 tggatgactg gcgcgatttt ctcaaggtta cgttggatct ttatgttcgc gaaagcaact
     6061 tcacacagct ggatgatgag ctgaaaaact ggattggtag ccgtttttca tcaaaattcg
     6121 tccgtaaccc ggaatcaaaa gatcctgaag ataatcagaa cagacgctgg cctcaaattc
     6181 gtaatggcaa cgtatcccat cgtttagcga agttactgat gctgggggct ggattcaaaa
     6241 ccgtcaatac ggcgactatt gatattatca atacatggct gaaagaagca tgggcccaac
     6301 ttaccggacc acttgcagta ctgaaacccg atggcaaccg tttttattta ccgaaagagc
     6361 atatgacttt ttctttaatc acggatgcct ggatttgccc ggtaaccaat aaaatcctgg
     6421 atacggcttt taaaggctta accccttatc tgcccaccca tatttcgttc gagcatctta
     6481 cccaggcgca atatgacact tttgtcgcgc agaaagtcac catgccggaa atctggaagc
     6541 tggatcgttc acaggaagac tacgcagagg gtctggcaaa agcacgagac tgggtcaaca
     6601 atgatccgct gatcgcacaa ttgcggtcgg aaaacgtctg gacagacatc aacgatcgtg
     6661 tcgtggaagg cggtttctac tatcgcactg ccgagcactc tgcccagcag tcgtcagaac
     6721 gcctgcaaag ctatgaaaag atgttcaaga acgggcaact aaatgttctc aactgttcca
     6781 ccaccatgga aatgggggtt gatattgggg gcattactgc ggtggtaatg aacaacgttc
     6841 cgccacatcc ggccaactat ttacagcgcg ccgggcgagc cgggcgtagt aaagaatcac
     6901 gcgcgatctc ttatacccta tgcaaaggta acccacacga ccagcaggtt tttgccaacc
     6961 cactctggcc atttgaaacc atgatcccgg cacccatggt cgcgatgaac tccgcccgac
     7021 tggtgcaacg tcacgtcaat gcattattac tgtctgactt tttatgtaac gttattggcg
     7081 aaaccgataa agagaaaacc agcctcgata gcctctggtt tttcggtgaa gatgacgggc
     7141 agtcaaaatg cgaacgcttt aaagtctggc tggaacgccc ggttcttgat atcgacacgg
     7201 cgctggaacg gctggtgaaa ggtacggcgt tgcatggcgc aagagccgaa catctgcggg
     7261 ataaaacaat caatgcgatc accttcctgc aacagcggtg gctctccgtt tatcgcgatc
     7321 tcgtgaccca ggagcgtgag tcgcagccgc aaacgcctta tcgtaagcgc atagagctgg
     7381 agaagaaacg gcactgtggc gagtatctgc tgcgcgatct ggctgccaga accttcctgc
     7441 cgggatacgg tttcccgaca gatgtagtga cgtttgataa cttcacgatg gaagattata
     7501 tccgcgaaaa atcgcagaaa agtcgtgata aaaaggaccg tgaagataac gtctcccgtt
     7561 ataaaggatt accttcccgt aacctcggtg ttgccattcg tgaatatgca ccgggagcag
     7621 agatcattct tgatggccgt gtcttccgct ctgccggtgt ctcactgcac tggcacaaca
     7681 ttaatgctga taccaatgaa gcccagcgtc tggattgcgc ctggcgctgt cacaaatgcg
     7741 ggaccattgg ctatgaagag gggatgagca gttccgggat gctcttttgc tccaacagcg
     7801 cctgcggcga aaaaataacc atggataatc gccgtcaggt gttgcagcct gccgggtttg
     7861 tcaccgatgc acacgctccg gtaacgaaca atattgaaac catgaaattc gtcccggttg
     7921 tccccgcctg ggtctttgtt aaagcagagc ccgtaccgtt gcctaacccg ctaatgggtt
     7981 atatggcttc tggggctgat gggcatgttt tccagcaaag tctgggggaa ggtggacacg
     8041 gttatgcatt gtgtctgagc tgtggccgtg cggagtcgat gctgaatgaa aatgatgctc
     8101 cgaaaagtat ggaagctcac tatcctccgc gtccgggtaa agcggaccgc gacagccaga
     8161 atcatcgtct gatttgcccc ggttcgacag cactcatgaa gaacgtcacc ctgggcgcac
     8221 tggcgcgcac cgatgtcttt gaaatggttc tgcgcaaacc acaaaatggc gagtatctgc
     8281 cagataacac agaggagggg cggattgtcg ccatgactct ggcggttgct ctgcgtcagg
     8341 cgctggcagg cgttctgggt atctccgccg cagaactcgg ctattctgta cgtccggtca
     8401 gactggagga tgggcaatcg gtattagccg ttcagcttta tgatgtgatc agtggtggtg
     8461 caggttttgc ctccagcgca ccggtacaca ttgaagctat attgcaggga atggtgaaac
     8521 agttaggctg ccgccattgt gatactgcat gtagtgaatg cctgcttgat tcccagactc
     8581 gccacgatca cgatttgctg gatcgcaaag ttgcgctggc ctggcttggc gatgacttca
     8641 cgtattacat cggtttaccg gatgaagaga cattctcgct gccggatgcc cgctactgcc
     8701 ctggcgctat tggcgatacg attcgccggg cgattaacga aggtgctgaa aagcttaccc
     8761 tatggatgac cggcgcgccc aatgagtggg atttatacgc ccgtcagttc cgcgcagcta
     8821 ttcagagcta tcggctaaaa gacaacgtcg aggtcgatct tgttatccct gcgggagtgg
     8881 atgacccgga tctattgcat gaactttcgc aatttactgc gctgggtgtg cggttgtgcc
     8941 atgttgagca ggagctgcaa ttaccgattg ttgcccaggt cacctttgcc gatcgcgtga
     9001 tgacgctggc ctctcgcagc cagcaggcaa cgattccggg gccggagtgg cacctgaatg
     9061 acgaactggt ggtgcgtagt ctcggttatc aaacggtaga actgaacgag tttattctgc
     9121 ccgctaaagc cgccaacgct gtcgagcggg tcaaagacat tcagatccac aaacagttga
     9181 atggaccact ttcgcagttt gggcaacgct tctgggacgt gctgtttaac gaccatgaag
     9241 aagcgcagtc actgatgaaa aacacgcgaa ttaccggcgt tcactatacc gatcgctatc
     9301 tgcaaaaccc ggttgcgctg gcgttgttgg gatcaattct gaaaccgcta aaaacaaagc
     9361 tgaccgatgg cgctgaagtg gcgctggata cgctgtttaa agataaagat cgccccggta
     9421 accggccttt ccatgactgg atgagcatag cggattttca ggatttcgct gatcagtggt
     9481 ttgctgctgc gctgggcagg ccgatagaac tgacggtgtt cgattcgccg cgcgatatcc
     9541 ctcaccatcg taaactgacg gtgacttttg aggatggtca ggtattgaag atccgcttcg
     9601 atcaggggat gggctactgg cgcatcaact ttgcatcgca atggcattac tttgatttcc
     9661 gcgatgacgt ttctttccag ttagtcaaaa tggctcaggc ctgcaaggaa gggaatgtgg
     9721 ccaacagcga agagagctgg gcaacggatg tgctggtgga ggtgaccgcc tcctgatgat
     9781 gtgccgctcc cgacgtcgtg tcgggagcgt ttttcaattc aggcaacttt ctgtttaccc
     9841 gcaattaccc caataaaatc ctgcacctgc ttttgtttac gcgccgacag cttgcacacc
     9901 tggcgcagcg actgcatcag ttcgctctct tcggcgacgg gtggttgggc ggtgaggaca
     9961 atacagcctt ccattacttt gacatctacc gccgtgccgg tggcaaaacc ggcggcttcc
    10021 agccactgac ctttcagggt aatggcggga atacggctgt aatccgggta gcggctcgca
    10081 taactcacgg ttaattgacg gttatttgcc ggggagactt ctgcttcgaa cggttgtgca
    10141 atagaatgcg tgtcagtcat gattactatt ctccataaat agtgattgtg atgagcggtg
    10201 cgggtgtgtt ggcgcacatc cgcaccgcgc taaatacctg gatatatcat cagtaaatac
    10261 aggaaaagtc cagctaaaaa tagaataaaa tgggcaattt ctggaatgaa taaaatatat
    10321 ttgcgtgagt tatgattggc gtgaaataat aaaaagcgca ccggaaaggt gcgccagaaa
    10381 ataatgatca ggatttttta cgcgaggctt ttttaccccc gctggctgcg cgttcagctt
    10441 tgattttctc cagcaacgcg gcggcgctgt tttcaccgct gattaattcc gggttttcgg
    10501 cccgccactg ggcagtgagc tcaccacgga atgcttttgc caggatggat tgcgtcagat
    10561 tgttgacgcg ggctaaggcg ttgttgacct gtttttctat ggtgtcggcg taggcgaaga
    10621 gttgctcgac gcggcgaacg atttcgcgtt gttcctccag cgatgggaga ggaataggta
    10681 actcttttaa tataccaaga tttaggatat ccatagtccc gccatgactg aatcctttaa
    10741 aaaagtcata tgctaagggg ccatctaaat acagctttac atattggcgt tgaatttcac
    10801 tatgtaatgc tatttttact aggcgtggat ttattatacc tgtctctgca agattagata
    10861 gtattaatgt ttttccgata gtaccaacta aactaataag aatatctcct ggtttggtgg
    10921 cgcaggattt caattgttga tatttatctt ctgtaattct ataagtttca agtgattcct
    10981 gtcctgagat aacttgctct tgaccataaa ctttatgacc agatttaaca cagtcagatt
    11041 ttttgagtgc cgaaccaaat ggcccagctt ttaacgcatt gttatcagtc tcacaaatct
    11101 gttcaaaaca tacccactcc caacaatcgg cagg
//
LOCUS       DAZFKF010000139        11133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFKF010000139 DAZFKF010000000
VERSION     DAZFKF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.141"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            161..1303
                     /gene="yghO"
                     /locus_tag="ACHKA1_004618"
     CDS             161..1303
                     /gene="yghO"
                     /locus_tag="ACHKA1_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO5682173.1"
                     /translation="MERDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1310..2074)
                     /gene="glcC"
                     /locus_tag="ACHKA1_004619"
     CDS             complement(1310..2074)
                     /gene="glcC"
                     /locus_tag="ACHKA1_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO5682174.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLSASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2325..3824
                     /gene="glcD"
                     /locus_tag="ACHKA1_004620"
     CDS             2325..3824
                     /gene="glcD"
                     /locus_tag="ACHKA1_004620"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO5682175.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSGEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3824..4876
                     /gene="glcE"
                     /locus_tag="ACHKA1_004621"
     CDS             3824..4876
                     /gene="glcE"
                     /locus_tag="ACHKA1_004621"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO5682176.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4887..6110
                     /gene="glcF"
                     /locus_tag="ACHKA1_004622"
     CDS             4887..6110
                     /gene="glcF"
                     /locus_tag="ACHKA1_004622"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO5682177.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKSDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6115..6519
                     /locus_tag="ACHKA1_004623"
     CDS             6115..6519
                     /locus_tag="ACHKA1_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO5682178.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6541..8712
                     /gene="glcB"
                     /locus_tag="ACHKA1_004624"
     CDS             6541..8712
                     /gene="glcB"
                     /locus_tag="ACHKA1_004624"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO5682179.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMASGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9067..10749
                     /gene="glcA"
                     /locus_tag="ACHKA1_004625"
     CDS             9067..10749
                     /gene="glcA"
                     /locus_tag="ACHKA1_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO5682180.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2615 a         2785 c         3085 g         2648 t
ORIGIN      
        1 aaatccacaa tagtcatgtt gatcggctaa cggtggcgct ggtgtatcgt aattattcat
       61 aaatattatt ttatttttcc ttatattcat tgcgtggata gttaaggtac cgtccgttgc
      121 tgtttatttt cagcattaat tatttgttat ccactaagct atggaacgcg atctgctgat
      181 gattaaaatt gaaaaagtaa ttaataaaaa tgaccttaag gcatttatcg cttttccgtc
      241 atcactttat cccgacgatc caaactggat acccccttta ttcattgaac gcaacgagca
      301 tttgtctgcg aaaaatccgg ggacggacca tatcatctgg caggcgtggg tggcaaaaaa
      361 agcggggcag atagttgggc gtattaccgc gcaaatagat accttgcacc gcgagcgtta
      421 cggcaaagat accggtcatt tcggcatgat tgacgccatt gatgatccgc aggtttttgc
      481 cgcgttgttt ggcgcagcgg aagcgtggtt gaagtcacaa ggtgcaagta agatcagcgg
      541 tccattcagc ctgaatatca atcaggaaag cggattactg attgaaggtt tcgatacacc
      601 accctgtgcg atgatgccac acggcaaacc gtggtatgcc gcgcatattg aacaactggg
      661 ttatcacaaa ggcattgatt tactggcgtg gtggatgcag cgaaccgatc tcactttctc
      721 tccggcgcta aaaaaactga tggatcaggt gcgcaaaaag gtgaccattc gctgcatcaa
      781 tcgtcagcgg tttgccgaag agatgcagat cctgcgtgag attttcaact ctggctggca
      841 gcacaactgg ggatttgtgc cgtttaccga acatgaattc gcgaccatgg gcgaccaact
      901 taaatatctg gtgccggacg atatgatcta catcgctgag attgattctg caccctgcgc
      961 gtttattgtc ggcttgccga acatcaacga ggcgattgcc gatctgaacg gatcgctctt
     1021 tcccttcggc tgggcaaaat tgctgtggcg tttgaaagtc agcggtgtgc gaactgcgcg
     1081 agtaccgctg atgggcgtac gcgacgagta tcagttcagc cgcatcggcc cggtgattgc
     1141 gctgttattg attgaagcct tacgcgatcc gtttgcccgc cggaagatcg atgcgctgga
     1201 gatgtcatgg atcctcgaaa ccaatacagg catgaataac atgctggaac gcattggggc
     1261 ggagccgtac aagcgttacc gattgtatga aaagcagatt taataccagc taactcaggt
     1321 tcatctccag cggcaccgcc gagcgaatca aatggtggcc ttccagctcg atatcgtgga
     1381 gattcttttt cacggtccgc acatgatcgc gtgctgcgcg ctgggcgacg tgcggcagcc
     1441 gctgcaacac cgcgttgtag atccgcgcat gctggcgatc gatctgcttt ttttgctgtg
     1501 gtcgatggta gagattattt acgctggcaa acactgaatt aaacatcaga tcggtcaatg
     1561 attgcagcgt aaacaccagc acctgattgt gagaagcctg acaaatggcg agatggaaag
     1621 cgtgatccaa ctgcgcatgt tcgatcagcg aaatctcttt gttgttctca ctggcggaga
     1681 gcattttttc ataacagcga gttatcacaa caaaatcagc ctgcgttccc agcgttgccg
     1741 ccagccttgc cgattcgccc tccagtaatg cgcgaacatc gagcagatcg tacagcgttc
     1801 gcggttgcgt actgaacaga tggatcagcg gactggtgtc ctgcacccga ttaagccgtg
     1861 cgacacgaga atcgcgaccc tgcgccgttt caataatccc gcgcccgcgc agcacggtca
     1921 gcccttcacg cagtgcggag cgtgagaagc cgagcttttc acacagtcga cgttccgagg
     1981 gaagcggctg accgaccttc agtacgccgt cgataattaa ccgttcgata ctctctgcaa
     2041 ccacttcgca aatagggcga cgttcatctt tcattcccgg acctcgtgca caggtaggac
     2101 caatttttct gttcttaact cgcaaaacac gcacatcacg taagtgatta tgttacatca
     2161 atttaacatt aagttaacca aggcaaggtc acagagctgg aaaaaaaatg gtctgaccgg
     2221 tagctaaaga gatagacgaa aacgaaaagc ccgcttaata actgttcaca gaagcagcgc
     2281 gcaaaaatca gctgccacac aacataacaa agcgaagcct actcatgagc atcttgtacg
     2341 aagagcgtct tgatggcgct ttacccgatg tcgaccgcac atcggtactg atggcactgc
     2401 gtgagcatgt ccctggactg gagatcctgc ataccgatga ggagatcatt ccttacgagt
     2461 gtgacgggtt gagcgcgtat cgcacgcgtc cattactggt tgttctgcct aagcagatgg
     2521 aacaggtgac agcgattctg gctgtctgcc atcgcctgcg tgtaccggtg gtgactcgtg
     2581 gtgcaggcac cgggctttct ggtggcgcgc tgccgctgga aaaaggtgtg ttgttggtga
     2641 tggcgcgctt taaagagatc ctcgacatta accccgttgg tcgccgcgcg cgcgtgcagc
     2701 caggcgtgcg taacctggcg atctcccagg ccgttgcacc gcataatctc tactacgcac
     2761 cggacccttc ctcacaaatc gcctgttcca ttggcggcaa cgtggcagaa aatgccggcg
     2821 gcgtccactg cctgaaatat ggtctgaccg tacataacct gctgaaaatt gaagtgcaaa
     2881 cgctggatgg cgaggcactg acgctgggat cggatgcgct ggattcgcct ggttttgacc
     2941 tgctggcgtt gttcaccgga tcggaaggta tgctcggcgt gaccaccgaa gtgacggtaa
     3001 aactgctgcc gaagccgccc gtggcgcggg ttctgttagc aagctttgac tcggtagaaa
     3061 aagccggact tgcagttggt gacatcatcg ccaatggcat tatccccggc gggctggaga
     3121 tgatggataa cctgtcgatc cgcgcggcgg aagattttat tcatgccggt tatcccgtcg
     3181 acgccgaagc gattttgtta tgcgagctgg acggcgtgga gtctgacgta caggaagact
     3241 gcgagcgggt caacgacatc ttgttgaaag cgggcgcgac tgacgtccgt ctggcacagg
     3301 acgaagcaga gcgcgtacgt ttctgggccg gtcgcaaaaa tgcgttcccg gcggtaggac
     3361 gtatctcccc ggattactac tgcatggatg gcaccatccc gcgtcgcgcc ctgcctggcg
     3421 tactggaagg cattgcccgt ttatcgcagc aatatgattt acgtgttgcc aacgtctttc
     3481 atgccggaga tggcaacatg cacccgttaa tccttttcga tgccaacgaa cccggtgaat
     3541 ttgcccgcgc ggaagagctg ggcgggaaga tcctcgaact ctgcgttgaa gttggcggca
     3601 gcatcagtgg ggaacatggc atcgggcgag aaaaaatcaa tcaaatgtgc gcccagttca
     3661 acagcggtga aatcacgacc ttccatgcgg tcaaggcggc gtttgacccc gatggtttgc
     3721 tgaaccctgg gaaaaacatt cccacgctac accgctgtgc tgaatttggt gccatgcatg
     3781 tgcatcacgg tcatttacct ttccctgaac tggagcgttt ctgatgctac gcgagtgtga
     3841 ttacagccag gcgctgctgg agcaggtgaa tcaggcaatt agcgataaaa cgccgctggt
     3901 gattcagggc agcaatagca aagccttttt aggtcgccct gtcaccgggc aaacgctgga
     3961 tgtgcgttgt catcgcggca ttgttaatta cgacccgacc gagctggtga taaccgctcg
     4021 tgccggaacg ccgctggtgg cgattgaagc ggcgctggag agcgccgggc aaatgcttcc
     4081 ctgcgagccg ccgcattatg gtgaagaagc cacctggggc gggatggtcg cctgcgggct
     4141 ggcggggccg cgtcgcccgt ggagcggttc ggtccgcgat tttgtcctcg gcacgcgcat
     4201 cattaccggc gctggaaaac atctgcgttt tggtggcgaa gtgatgaaaa acgttgccgg
     4261 atacgatctc tcacggttaa tggccggaag ctacggttgt cttggcgtgc tcactgaaat
     4321 ctcaatgaaa gtgttaccgc gaccgcgcgc ctccctgagc ctgcgtcggg aaatcagcct
     4381 gcaagaagcc atgagtgaaa tcgccgagtg gcaactccag ccattaccca ttagtggctt
     4441 atgttacttc gacaatgcgt tgtggatccg ccttgagggc ggcgaaggat cggtaaaagc
     4501 agcgcgtgaa ctgctgggtg gcgaagaggt tgcgggtcag ttctggcagc aattgcgtga
     4561 acaacaactg ccgttcttct cgttaccagg taccttatgg cgcatttcat tacccagcga
     4621 tgcgccgatg atggatttac ccggcgagca actgatcgac tggggcgggg cgttacgctg
     4681 gctgaaatcg acagccgagg acaatcaaat ccatcgcatc gcccgcaacg ctggcggtca
     4741 tgcgacccgc tttagtgccg gagatggtgg ctttgccccg ctatcggctc ctttattccg
     4801 ctatcaccag cagcttaaac agcagctcga cccttgcggc gtgtttaacc ccggtcgcat
     4861 gtacgcggaa ctttgaggag caggctatgc aaacccaatt aactgaagag atgcggcaga
     4921 acgcgcgcgc gctggaagcc gacagcatcc tgcgcgcctg tgttcactgc ggattttgta
     4981 ccgcaacctg cccaacctat cagcttctgg gcgatgaact ggacgggccg cgcgggcgca
     5041 tctatctgat taaacaggtg ctggaaggca acgaagtcac gcttaaaaca caggagcatc
     5101 tcgatcgctg cctcacttgc cgcaattgtg aaaccacctg tccttctggt gtgcgctatc
     5161 acaatttgct ggatatcggg cgtgatattg tcgagcagaa agtgaaacgc ccactgccgg
     5221 agcgaatact gcgcgaagga ttgcgccagg tagtgccgcg tccggcggtc ttccgtgcgc
     5281 taacgcaggt agggctggtg ctgcgaccgt ttttaccgga acaggtcaga gcaaaactgc
     5341 ctgctgaaac ggtgaaagct aaaccgcgtc cgccgctgcg ccataagcgt cgggttttaa
     5401 tgctggaagg ctgcgcccag cctacgcttt cgcccaacac caacgcggca actgcgcgag
     5461 tgctggatcg tctggggatc agcgtcatgc cagctaacga agcaggctgt tgtggcgcgg
     5521 tggactatca tcttaatgcg caggagaaag ggctggcgcg ggcgcgcaat aatattgatg
     5581 cctggtggcc cgcgattgaa gcaggtgccg aggcaatttt gcaaaccgcc agcggctgcg
     5641 gtgcgtttgt caaagagtac gggcagatgc tgaaaagcga tgcgttatat gccgataaag
     5701 cgcgtcaggt cagtgaactg gcggtcgatt tagtcgagct tctgcgcgag gaaccgctgg
     5761 aaaaactggc aattcgcggc gataaaaaac tggccttcca ctgcccgtgt accctacaac
     5821 atgcgcaaaa gctgaacggc gaagtggaaa aagtgttgct tcgtcttgga tttaccttaa
     5881 cggacgttcc cgacagccat ctgtgctgcg gttcagcggg aacatatgcg ttaacgcatc
     5941 ccgatctggc acgccagctg cgggataaca aaatgaatgc gctggaaagc ggcaaaccgg
     6001 aaatgatcgt caccgccaac attggttgcc agacgcatct ggcgagcgcc ggtcgtacct
     6061 ctgtgcgtca ctggattgaa attgtagaac aagcccttga aaaggaataa caaaatgaaa
     6121 actaaagtca ttcttagcca gcaaatggcg agtgcaatta ttgccgcagg tcaggaagag
     6181 gcgcagaaaa acaactggtc tgtttccatt gctgttgccg atgacggcgg tcatctgctg
     6241 gcgttaagtc gcatggacga ttgcgcgccg attgcggctt acatctccca ggagaaagcg
     6301 cgtaccgccg cgctggggcg tcgtgaaact aagggctatg aagagatggt gaacaacgga
     6361 cgtaccgcgt tcgtgactgc gccgttatta acgtcgctgg aaggcggcgt accggttgtt
     6421 gttgatgggc aaattattgg tgccgtgggc gtttctggtt taaccggagc acaggatgcg
     6481 caggtcgcga aagcggcagc agcggtgttg gcgaaataag cgaaaacgag gagagaaaca
     6541 atgagtcaaa ccataaccca gggccgttta cgcattgacg ccaattttaa acgttttgtg
     6601 gatgaagaag ttttaccggg aacagggctg gacgctgcgg cgttctggcg caattttgat
     6661 gagatcgttc acgatctggc accagaaaat cgtcagttgc tggcagaacg cgatcgcatt
     6721 caggcggcgc ttgatgagtg gcatcgcagc aatccggggc cggtaaaaga taaagcggcc
     6781 tataaatctt tcctgcgtga actgggctac ctggtgccgc aaccggagcg cgtgacggtg
     6841 gaaaccacgg gcattgacag cgaaatcacc agtcaggcgg ggccacagct ggtggttccg
     6901 gcaatgaacg cccgctacgc gctgaacgcg gcgaacgctc gctggggctc actgtacgat
     6961 gcgttatacg gcagcgacat catcccgcag gaaggggcga tggccagcgg ctacgatccg
     7021 caacgcggtg agcaggttat cgcctgggtt cggcgtttcc tcgatgaatc tctaccgctg
     7081 gaaaacggca gctatcagga tgtggtggcg tttaaggtgg tcgataaaca attacgcatc
     7141 cagttgaaaa atggtaaaga aaccacgtta cgtaccccgg cgcagtttgt cggttaccgt
     7201 ggcgatgccg ctgcgccgac ctgcattttg ctgaaaaata acggcctgca tattgaactg
     7261 caaattgatg ccaacgggcg gattggcaaa gacgatccgg cgcacatcaa cgatgttatc
     7321 gtcgaagcgg ccatcagtac cattctcgac tgcgaagatt cggtcgcggc ggttgatgcg
     7381 gaagataaaa tcctgctgta ccgcaacctg ctgggcctga tgcaggggac tctgcaagag
     7441 aaaatggaga aaaacggtcg acaaatcgtg cgtaaactga atgacgatcg tcattacacc
     7501 gccgccgatg gctctgaaat ttctctgcac ggacgctcgc tgctgtttat ccgcaacgtg
     7561 ggtcatttga tgaccattcc tgtgatttgg gacagcgaag gcaatgaaat cccggaaggc
     7621 attcttgatg gcgtcatgac tggcgcgatt gccctctatg atttaaaagt gcagaaaaac
     7681 tcgcgcactg gcagcgtcta tattgtgaaa ccgaaaatgc acggtccgca ggaagtggcg
     7741 ttcgccaaca aactgtttac ccgcattgag acaatgctcg gtatggcacc gaataccctg
     7801 aaaatgggca ttatggatga agaacgtcgg acctcgctga acttgcgtag ctgtatcgct
     7861 caggcgcgca accgcgtggc gttcatcaat accggtttcc tcgaccgtac cggcgatgaa
     7921 atgcattcgg tgatggaagc tggcccgatg ctgcgtaaaa accagatgaa atcgacgccg
     7981 tggatcaaag cctacgaacg taataacgtg ctttccggtc tgttctgtgg gctgcgcggt
     8041 aaagcgcaaa ttggtaaagg catgtgggca atgccggacc tgatggcaga catgtacagc
     8101 cagaagggcg accaactgcg tgccggggca aacacagcct gggttccgtc accaaccgct
     8161 gctacgctcc atgcgctgca ctaccaccaa accaacgtac agagcgtgca agccaacatt
     8221 gcccagaccg agttcaatgc tgaatttgaa ccgctgctgg acgatctgct gactattccg
     8281 gttgctgaaa acgctaactg gtcggcgcaa gagatccaac aagagctgga taacaacgtg
     8341 caggggattc tggggtacgt ggtgcgctgg gtggagcagg ggattggttg ttcaaaagtg
     8401 ccggatattc acaatgtggc gctgatggaa gaccgcgcaa cgctgcgtat ctccagccag
     8461 catatcgcca actggttacg tcacggtatt ctgaccaaag aacaggtgca ggcgtcgctg
     8521 gagaatatgg cgaaagtggt tgatcagcaa aacgctggcg atccggctta tcgtccgatg
     8581 gcggggaatt tcgctaactc gtgtgctttt aaagctgcca gcgatttgat cttcctcggc
     8641 gtgaaacagc caaacggtta taccgaaccg ttattacacg cctggcgttt acgcgaaaaa
     8701 gaaagtcatt aatagcgtca aaaaagcccc ggcaagtttt attgtcgggg ctatgatagt
     8761 aactgcggta gtacatttat cccggaaata accggagcga ttatcagggt ttaacaatat
     8821 taaatacccg tagcgcatta ctagcgggcc taaaacaaca atatggacat cttaacgata
     8881 ctctgcgcct gcgtgtgcgg aacagaaacc cttacccgat atacttgtta tgccagaggt
     8941 agcaattgcg ttagcggcgt actgttgctg taaagtataa cgcctatatt ttacagttca
     9001 gtcctctttt gcttattaca accttaagta tatcaagcat ataaagataa taagagactg
     9061 aacaatatgg ttacctggac ccaaatgtat atgccgatgg gaggactggg gctatccgct
     9121 ctggtcgccc tgatcccgat aatattcttc ttcgttgcac tcgcggtatt acgtctgaaa
     9181 ggacatgtcg ctggagcaat aacccttata ttatctatcc tgattgcaat attcgccttt
     9241 aaaatgccga ttgatatggc atttgctgct gcgggctatg gctttattta tggattatgg
     9301 ccaattgcat ggattattgt cgcggcggta ttcctctata aattaaccgt tgccagcggg
     9361 cagttcgata ttatccgcag ctcggttatc tccatcaccg acgatcagcg tttgcaggtg
     9421 ttactgattg gtttctcctt tggtgcgttg ctggaaggag cggctggctt tggtgcgccg
     9481 gtggcgatta ccggtgcgct gctggtgggc ctgggcttca aaccgttata cgcggcgggg
     9541 ctgtgtctga ttgccaatac tgcgccggtg gcgtttggtg cgttgggcgt gccgattctg
     9601 gtcgccggtc aggtaacggg aatcgatccg ttccacattg gcgcaatggc gggacgtcag
     9661 ttaccgttcc tgtcggttct tgtgccgttc tggctggtag caatgatgga cggctggaaa
     9721 ggggtgaaag agacgtggcc agcggcgctg gttgctgggg gaagcttcgc tgtcactcag
     9781 ttctttacct ctaattatat tggtccggaa ctgccggata ttacttcggc gctggtgagt
     9841 atcgtctcac tcgctttatt ccttaaagtc tggcggccga aaaataccga aacggcaatc
     9901 agcatgggac aatccgcagg tgcgatggtg gtaaataagc catcttctgg cggtcccgtg
     9961 ccttcagaat atagtctggg gcaaatcatt cgagcgtggt caccgttttt aatcttaacg
    10021 gtgctggtca ccatctggac catgaagccg tttaaagcgt tatttgctcc gggcggcgcg
    10081 ttttattcac tggtgattaa tttccagatc cctcatttgc atcaacaagt gttgaaagcg
    10141 gcacccattg tcgcccaacc aacgccaatg gatgcggtgt ttaaattcga ccccctctcg
    10201 gctggcggca ccgctatttt tattgcggcg attatctcta tcttcatcct cggtgtgggg
    10261 atcaagaaag gtattggcgt ctttgccgaa acgctaatta gtctgaagtg gccgatactg
    10321 tcgattggca tggtgctggc gttcgccttc gtcaccaact attctggcat gtccaccacg
    10381 ctggcgctgg tactggcagg tacaggcgtg atgttcccgt tcttctcacc gtttctcggc
    10441 tggctgggcg tattccttac cggctcggac acctcctcta acgccctgtt tggttcactg
    10501 caatcgacca cggcgcagca aatcaacgtc tctgacactc tgctggtggc agcaaatacc
    10561 agcggcggcg taactggcaa gatgatctcc ccgcaatcta tcgccgtggc ctgcgccgcg
    10621 acgggcatgg tgggccgaga atctgaactg ttccgctaca ccgtgaagca cagtctgatt
    10681 tttgccagcg ttatcggcat tatcaccctg ctgcaggcgt atgtgtttac cgggatgtta
    10741 gtctcgtaaa tcaataaagc cggatgatcg tcaatatcat ccggcttcat ttacattatt
    10801 ctgtcttatt aattgaaaat catttctcgc tttattatcc atgcagcaat aatgttttgc
    10861 gttcagataa cctgctaaat aattaagagc gtgagaacat ctattttttt atcacgcaaa
    10921 acaagtctgt attaattatg ttcgcttgat gagcaaacac cgttctatct gttgcttttg
    10981 caatcttaat cgttaaaact accaacgaat ttatatagtt acgttgtgaa atgttgttaa
    11041 ttttttgttt attgaaattt cgtaaaaata gccagataga catcgtatag gggttattct
    11101 tataaatttc acctgttttc tatcaatgat gtc
//
LOCUS       DAZFKF010000140        10790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFKF010000140 DAZFKF010000000
VERSION     DAZFKF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.142"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            308..1129
                     /locus_tag="ACHKA1_004626"
     CDS             308..1129
                     /locus_tag="ACHKA1_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFO5682181.1"
                     /translation="MKPNSTGIAARMMLSIDRARISEALLNNRQLQSTPLQIRYPQGV
                     REALGIMSEQLSLSVSDLTRILVEDALTEMFLPAENIVHRLICRTEHIMQAHDVCVTT
                     MTTLLAPWNIRPAVFRDPDKLADYLTGEILTALADWFYLNPEWLNGRVRYPLSRAGEW
                     PATQDAFCQLISASDNVDIIIWHSFPFAGVHSQEYCGVLFRKKKEINKAIIYPVLSLS
                     PVRMNKEKENWFHTARNLYAKTSVRTVTLRPAQAEYLMTGKILPAELFSIPLLPW"
     gene            1219..2421
                     /locus_tag="ACHKA1_004627"
     CDS             1219..2421
                     /locus_tag="ACHKA1_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO5682182.1"
                     /translation="MMMNMHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGPEDDFSLLLPSVGGQLSDPGNISDNADKISDDWKAFDRAID
                     SQSNVSQTAARLKTRLQDFRHTHADAQVGTSAVATLPGDTLAAALMLKSYGTVSVDGK
                     VSDADLNYLESIANSNGQDVDKDRLTSQAFARAALVTDVGIALATELETAGQKWSLGF
                     TPKFQRVDLFNYNVLVKNYDSSAFKGDRYHNTRNGINADIGASMDLDDNWTLGLVAQN
                     LLPRSIETKEVNGVTETFRIRPQVTAGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AIGAEFNAWKWAQLRAGYRQNLASNDGSAFTAGLGISPFDVVHIDVAGIAGTDNNYGA
                     VAQLQFTF"
     gene            2561..2731
                     /locus_tag="ACHKA1_004628"
     CDS             2561..2731
                     /locus_tag="ACHKA1_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682183.1"
                     /translation="MFNKNKTHIENKAFMVEPFRDPTANECNVVIANDICIRVALSPS
                     EVFQPFSKNTNQ"
     gene            2780..3232
                     /locus_tag="ACHKA1_004629"
     CDS             2780..3232
                     /locus_tag="ACHKA1_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081199.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFO5682184.1"
                     /translation="MNRMIPAGCLAVLCLNGCHLPPDNTAPTRKYGAASDVAVARHAQ
                     YQLWGQGIYSAALPERQSGGSLKAHSDRVSFDWEGDALELLNELARLRGQQFSYSGVR
                     LPLPVSLHVRDMTFADVLRLTEAQTAWRATLSQSAGRLHLSFMTPEKK"
     gene            3229..4065
                     /locus_tag="ACHKA1_004630"
     CDS             3229..4065
                     /locus_tag="ACHKA1_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFO5682185.1"
                     /translation="MKTHLCLLLSALLLTGCAGQHHRSSAVMTENAPPPDIRAWLTPS
                     GNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIRPDQIRTASRVWTILRPERFVSNPPGWRDWLLKGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRLNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQMQTVTGNGQKLITGDRVRRLKTHAEFTLQKSRWRPLVSTEGEKNDI
                     TR"
     gene            4049..5197
                     /gene="traJ"
                     /locus_tag="ACHKA1_004631"
     CDS             4049..5197
                     /gene="traJ"
                     /locus_tag="ACHKA1_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFO5682186.1"
                     /translation="MISPDDFGTFPFSRFTAEEFRHFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSVNPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKAISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRHILKNFPDRKIVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVMTDELRNTLSGQS
                     HATWGHYIDEIIRQEKRRIRDQVLTMYQNDRIEKSEAALFIPAGEFPR"
     gene            5194..5484
                     /locus_tag="ACHKA1_004632"
     CDS             5194..5484
                     /locus_tag="ACHKA1_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO5682187.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFMLLGYSGWTVRTLCIRLYSLLQGKQASGRPWWYRHRTELPREWHGPE"
     gene            5548..9564
                     /locus_tag="ACHKA1_004633"
     CDS             5548..9564
                     /locus_tag="ACHKA1_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFO5682188.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAE
                     AGTDLSLLKRWLPRPQEMSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAQSGAYKAYLDGRPSGWYRDYRSADNSPTTWTFSRAENIDPLARLHLKASAQQNR
                     DEKERKLQQQYNKQAGYARSYLNGLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKGSEKSGNWFAFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTIDADNMINVAKNARKIWTDSPFVFCADNDHQRKINKGVSSAKKAAASTH
                     GEVIIPTFTEAEKAQGLTDFNDLDASRGRDAFQRAMNAQLEYIGIPTPNNDTTDRREA
                     IVIGNLIFTPVKDEKPQVSPENRQSTAPEPAVSEQNHPYDIPAEETDNQAYEDDYAAY
                     ASLQSVTAGTASPNEPQVNAVSAIDSNAKNTSAATTDDRNVLAQETNADATVTTTAHD
                     TGTAETDSPSEPAEIQPVQQASPEPSAKDKPQENVVSATDNDAENTSSTTTALRSYDT
                     PETSVSDATGTQHTATTQPEEPQQVSMGSADDWIAFAEDLNADAPGNATVHSTDNAET
                     KTLSEPAGMHPVQRATPEPTEENKPQEEGVMSATDTGEESAAAAIDDDLNTVAEDLNA
                     DVTVMTTALHTDTAETASPSEPAEIQPVQQATPEPTAEDKPKEERPVSATETSTENEA
                     ARPETPSLFSRLRRFLTGGKSRTAEPPVIQENTLTEAETATEAAAPPSPPETSPEDTS
                     SAEPDTIIFAPRRPNSPQRHNLDEIIKNLTYDELPDNTALCRVDGEPAFLDKLNRLEM
                     VDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIVEYDIKVRMKLPVQRA
                     ALEKLRQEKGIAHDAIVTHRPTPELNRLSPEETTMAQPAPPVSPKAETEQTAGSTAET
                     PHPEAPVTAKAEAPRAGTPEQPAASSSAPEKAPVSAQPQQPDTPEEEPPGKLRPGESV
                     TAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVKNHQKGNPVTLTLQAR
                     ETFRLSGETRDRIRNIWAMTPVSNGITVDNDHPHAGQHILEFPMKTFSQVMSQMQQHW
                     PQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAPNELIPAIASADKDNR
                     QLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINALTDNGLQFAGYGEAVN
                     HDENTHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSLGFEQTWKEWSDSQKA
                     EDRQEKTLQQAQSHSPGL"
     gene            9581..9931
                     /locus_tag="ACHKA1_004634"
     CDS             9581..9931
                     /locus_tag="ACHKA1_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO5682189.1"
                     /translation="MNMKKTLLVILTSISLASPAAVTATCRAGAAAEAGSKAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDVSITLPQFPSLDKILRDLENQVCDAVVDRVNE
                     HLPGNIDPWKDYNL"
     gene            9943..10638
                     /locus_tag="ACHKA1_004635"
     CDS             9943..10638
                     /locus_tag="ACHKA1_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFO5682190.1"
                     /translation="MSEAHPTSPVSPETDNSGDIYKQTLSTIALSEQRAALVPGLVRA
                     LLCSSAALVISLTGNIIQYWHNSNVTREYFATDNGRLVRLAPTSQPAWSQSDVMTFGS
                     MTLSEAFNLDFVHYRKQITTLAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTATIG
                     AGTLVNQGQMENGIWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAASGT"
     gene            10649..>10790
                     /locus_tag="ACHKA1_004636"
     CDS             10649..>10790
                     /locus_tag="ACHKA1_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFO5682191.1"
                     /translation="MQIHHLLGALLIVCGTVTAADNPGWQNARTPQAGGKTGQAPGNA
                     PAG"
BASE COUNT         2789 a         3116 c         2670 g         2215 t
ORIGIN      
        1 aatatccctt ataatctcca gtttttcttt atgctgttta attttcatac gaacctcgtt
       61 gatttcatcc cgaaaaggaa aagattatat aacgaaatac tttcaatctc ctgactccgt
      121 ctgctctgca gcataatgcc agaccagcgg catgctaaat cacgtcctgt ccgaatcctt
      181 ttcattcacg cttaaaacag agttaaaagc actcttgaaa cacactcgat cataacgatc
      241 gaaataactc gtttccgttc agtaaacacg ctctttagac ttaacatatg aaccgcacgg
      301 aggtgaaatg aaacccaaca gcacaggcat tgctgcccgc atgatgctct ccattgacag
      361 ggcccggatt agcgaagctc tgctcaataa ccgccagttg cagtccactc ccctgcagat
      421 ccgttatccg cagggcgtca gagaagcact cggaataatg agcgagcaac tctccctttc
      481 tgtatccgac ctgacgcgta tcctggtcga agatgcatta actgaaatgt ttctcccggc
      541 tgaaaacatc gttcaccgtc ttatctgccg gacagaacac atcatgcagg cacatgatgt
      601 ctgcgtaaca acgatgacaa cgttactggc tccctggaat atccgtcctg ccgttttccg
      661 tgacccggac aaactcgcag attatctgac aggagagatt ctgactgctc tggctgactg
      721 gttttatctc aacccggaat ggctgaacgg cagggtacgt tatcctttaa gccgtgccgg
      781 tgagtggcca gcaacacagg atgcgttctg ccagcttatt tcagccagcg ataatgtgga
      841 tattattatc tggcacagtt ttccttttgc cggtgttcat tcacaggaat attgcggtgt
      901 actgttccgc aagaaaaaag aaattaataa agccatcatt tatcctgtac tttctttatc
      961 acctgtaaga atgaataagg aaaaagaaaa ctggtttcac actgcccgca atttatatgc
     1021 aaagacatcc gtccggaccg taacgcttcg tccggcacag gcagaatatc tgatgacagg
     1081 aaaaatatta cctgctgagt tattcagtat accgttatta ccctggtaat aacgcagaga
     1141 aaaaaccaat aaatttttaa tggcacagtc gtgtcatacc gttgctgtac ccaaaacacc
     1201 tcaaaccagg aaactgaaat gatgatgaat atgcacatta cccgaactct catcgcatct
     1261 gctgttttat tttcctttaa ttcaacagcc gcaacttcat attttgaagc ccgcaatgat
     1321 gcaatggggg gaaccggggt agcctcctct cactacggtg tggcacctct ggcaaacccg
     1381 gccctgctga caaaagccgg tccggaagat gacttcagtc ttctgcttcc gtccgttggt
     1441 gggcaattgt ccgatccggg taatatcagt gacaacgcag acaaaatcag tgatgactgg
     1501 aaagcctttg atcgtgccat cgacagtcag agcaacgtgt cgcagactgc tgcacgtctg
     1561 aagacgcgac ttcaggactt ccgtcacacg catgctgacg cacaggtggg aacatctgct
     1621 gtggcgactc ttcccggtga caccctcgct gccgctctga tgctgaaaag ctacggtacg
     1681 gtcagtgtcg acggtaaagt cagtgatgcc gacctgaact atctggaaag cattgcaaac
     1741 agtaacggtc aggatgtgga taaagacaga ctgacctctc aggcttttgc ccgtgccgca
     1801 ctggtcaccg atgtgggtat tgctctggct actgaactgg aaacggccgg acaaaagtgg
     1861 tctctgggct tcacaccaaa attccagcgt gttgacctgt ttaactacaa cgtgctggtt
     1921 aaaaattacg acagcagcgc tttcaaaggc gaccgttacc acaacaccag aaacggcatt
     1981 aacgctgata tcggggccag catggacctg gatgacaact ggacactggg tctggtcgca
     2041 cagaacctgc tcccccgcag tattgagaca aaggaagtga acggtgtcac tgaaactttc
     2101 aggatccgcc cgcaggtgac tgccggtgtc tcctggcaca acgacatgtt cactacagca
     2161 ctggatgttg acctgacccc ggccagtggc ttcacatcag acagtaaacg tcagtttgct
     2221 gccattggtg ctgaatttaa tgcctggaaa tgggcacagt tacgtgcagg ctaccgccag
     2281 aatctggcca gcaatgatgg cagtgcgttc accgccgggc tgggtatctc accatttgat
     2341 gtcgtccata ttgatgtggc gggtatagca ggcacagaca acaactacgg tgccgttgca
     2401 caactgcagt tcacattctg atcatctctg ccccggtact gaccggggta tccagacctg
     2461 catcctcgcg ttttccgacc atcaaacgac acagccagac ctgtagtgct gcctctgatc
     2521 tggctgttat ctgacatatc attttcgcat gaggaaatgc atgtttaaca aaaataaaac
     2581 gcatatcgaa aacaaagcct ttatggtgga accgttccgg gatccgacag ctaacgaatg
     2641 taatgtcgtg atagcaaacg atatttgtat ccgggtagca ctgtcaccct ctgaggtgtt
     2701 ccagccattc agcaaaaaca cgaaccagta aatcctcccc acaacgcttt cttctttcac
     2761 tgctcaggaa aaatacacca tgaaccgtat gatacctgcg ggatgtctcg ccgtactctg
     2821 cctgaacggc tgccatctcc ccccggataa tacagctccg accagaaagt atggtgctgc
     2881 ctctgatgtc gccgtcgccc ggcatgcgca gtatcagcta tgggggcagg gaatttactc
     2941 tgcagccctt ccggagcgtc agtccggcgg cagtctgaag gctcacagcg accgtgtttc
     3001 ctttgactgg gaaggcgatg ccctggaatt gctcaacgaa ctggcccgtc tgcgcggcca
     3061 gcagttcagc tacagcggcg tccgtctccc cttacccgtc tcactgcacg tccgcgacat
     3121 gacctttgct gatgtccttc gtctcactga agcccagact gcctggcggg caacactctc
     3181 gcagtcagcc gggcgtttac atctgtcttt tatgacgccg gagaaaaaat gaaaacacac
     3241 ctgtgtctcc tgctgtctgc tctcctgctt acaggctgtg ccggacagca tcaccgttct
     3301 tctgccgtca tgacagaaaa cgcaccgccc ccggatatcc gtgcatggct gacgccctcc
     3361 ggaaaccagg atgatggtat ttcagatacc cgtcgtcaga tgctcacgga tgccggcaga
     3421 acgctgggat tccgtggagg aaaagcacag cgctcatggg aactgattca ggcactgaat
     3481 gcccgggaaa gtaccctgaa tgccctgtat gatttccggc ctctcatcag caaggaaggc
     3541 tggctgcctc ctgttatcga tgaagcgcag gatgttgcac acatcagacc ggaccagata
     3601 agaacagcct cccgtgtctg gaccatcctc cgcccggaac gctttgtcag taatccacct
     3661 ggatggcgtg actggctgct gaaaggactc tcaaccaccg ccacaccagg cactgaaggc
     3721 cgggttgtgc cggaagaccg ggcacaacgc agactatggg aaaacgcctt acgtcaggga
     3781 tggcaggaag ggcgcctcaa cgcagatctc acactggaag ctaatcagaa gcgcctgacc
     3841 cgtgattacc ggggcatgat gctttacgcc cttctgtggc gtcagggcat gatcacacgt
     3901 ccagatgtca cagagcagat gcagacggtt accggcaacg ggcaaaaact catcactggc
     3961 gatcgcgtcc gccgcctgaa aacacatgct gaatttacgc tgcagaaatc gcgctggcgc
     4021 cctcttgtca gcaccgaagg agagaaaaat gatatcacca gatgacttcg gtaccttccc
     4081 cttctcccgt tttacggcag aagagtttcg ccattttttt gcctggtgtg cccgccaccg
     4141 cgtcagtgat attgatctga ccggcggttc accggtatcc gtcagtcgct ttggacggcg
     4201 ggttcgctca tccgtaaatc cccttccgtc ctccatcatg atggcactga gtgatgagct
     4261 gtttgggcgg gaggttcttc cccgggttct gtcaggtcat cccgtggatc gtaccattca
     4321 ggtcagcgga gatgcctccg gtcgctatgg cctgcagcga ggtcagcgtg ttcgcctgcg
     4381 ctgccacctg atacagggaa catcggcaac cgaagaaaaa gccatttccg tcaccatgcg
     4441 ggtgatcccg tcagatattc cggacatcct cactatgaat attgaaccgg atctgctgga
     4501 tgccatgctc agaaaaaccg gcctgggctt catctgcgga gaaaccggct ccgggaaatc
     4561 cacactggcg gcggcactgt atcgccacat tctgaaaaat ttccccgatc gcaaaatagt
     4621 gacctatgaa gatccggtgg aatacattct gggcggagag aatgaccttc tgcctcccca
     4681 tcaggccgaa atcgggcgcg atgtggaaag ttttgctgca ggacttcgct ctgccgttcg
     4741 ccgtaatccg gacatcattg gtatcggtga aatcagggat tatgaaacag cagatgccgc
     4801 agtccaggcg ggtaataccg gacacttttg tatcggcacc atgcacacaa aatcacccgg
     4861 agaaaccttt gcccgcctgc tgggattgtt tcctcctgaa atcagggatt cgatggctgc
     4921 agccacgctt tccctcgtac agttcattct ggtgcaggta ctggtcagaa caaatgacgg
     4981 cggcaggcag gctgtccggg aatacatcgt catgactgac gaactgcgca atacgctctc
     5041 cggacaatcc catgctacat ggggacatta cattgacgaa atcattcgtc aggaaaaacg
     5101 gcggataagg gatcaggttc tgaccatgta tcagaacgac cgcattgaga aatcagaagc
     5161 agcgctcttc atcccggccg gagagttccc ccgatgaaac gcgaaggctt ctggcgggat
     5221 gtaacaccac cgctcaacat ttatggtgtc cccctgccgt ttaccatgat ttacctgctg
     5281 ttgtttccgt atccgtcaaa aacgacattc tgggtgtgca gcggcattct gctgtttttt
     5341 atgctcctgg gatattccgg ctggacagtc cgcaccctct gtatacgtct gtacagcctg
     5401 cttcagggta agcaggcatc gggacgtccc tggtggtacc gacaccgcac agaattaccc
     5461 cgtgaatggc acggaccaga atagccctct tttgtccatc atcccggcgt atcacaacgc
     5521 cctcatattt aatccccgga gaacgacatg aagtccagat atcgtacctg gcttgctgtc
     5581 ccgcctgagg agacagaagc cgtaaaaaat gcagtccccc cgttaaatgg ccgcaaagcg
     5641 gtagcatggg atccagagaa aaaactctgg tatgccgagg cgggtaccga cctttcactg
     5701 ctgaagcgct ggttaccccg tccacaggaa atgtcgatgg atgccggcga tccggtcaca
     5761 gaatttgctc aggtcctgga aaacgccggg ctggttattc agggattgcc acagatggat
     5821 ggcgccatac acagagtcgc cacccgggat gacaaaaaag gggcccaaag tggcgcatac
     5881 aaggcctatc ttgatggccg cccctccggc tggtaccgcg attaccggag cgctgataac
     5941 agtcccacaa cctggacatt ctcccgcgca gaaaatatcg atcctctcgc cagactgcat
     6001 ctgaaggcat ccgctcagca aaatcgcgat gagaaagaac gtaaactaca gcaacagtac
     6061 aacaaacagg ccggctatgc ccgctcctat cttaacgggt tgccccaggc aacagcacat
     6121 gaatacctga cacgcaaggg catccgggcc gcgcctggcg tgcggctgaa caataaaaat
     6181 gagctggtta ttcccttcag caacggccgg ggggaaatac gttcgtacca gcgtattccg
     6241 gtcaccggtg ggaaggatgc ccgcattctg aaaggctctg agaaaagcgg caactggttc
     6301 gccttcggaa ccccggaaaa tggccgtccc ctgttatttg cggaaggtta cgctactgcc
     6361 gcctccctgc acgaagcaac cggccttcct gttctgatga caattgatgc cgacaatatg
     6421 attaatgtgg caaaaaatgc ccgcaaaatc tggactgaca gtccctttgt cttctgtgcc
     6481 gacaacgacc atcaacggaa aattaataaa ggtgtttcaa gtgcaaaaaa agctgccgca
     6541 agcacgcatg gcgaagtcat tattccgacc tttacggagg cggaaaaagc acagggactg
     6601 accgatttca atgacctgga cgccagccgg ggacgagacg cctttcagcg agccatgaac
     6661 gcacagttag aatatatcgg tatccccacg cccaacaatg acactactga ccgccgtgaa
     6721 gctatcgtga tcggcaacct gatattcaca ccggtaaaag atgagaagcc acaagtgtcc
     6781 cctgaaaaca gacagagcac agcccctgaa ccggcagtgt cggaacagaa tcatccttat
     6841 gacatccctg cagaagagac agacaatcag gcatatgaag atgactatgc agcatatgca
     6901 tccctgcagt cggtaactgc agggacagcc tccccgaatg aaccgcaggt gaatgctgtt
     6961 tctgccattg acagcaacgc aaagaatacc tctgccgcca ccactgacga ccgaaatgta
     7021 ttagcacaag aaaccaatgc tgacgccacg gttacgacca cggctcacga caccggcaca
     7081 gcagaaacag attcaccttc tgaacctgca gagattcagc cagtgcagca ggccagtcct
     7141 gaaccatcgg ctaaagataa accacaggag aacgtagtgt ctgctactga taatgacgca
     7201 gagaacacct cttccaccac cactgccctc cggagttacg acacaccgga aacatccgtc
     7261 tccgacgcaa ccgggacaca gcacaccgcc acaacgcaac ctgaagaacc acagcaggtc
     7321 tccatgggca gcgctgacga ctggattgca tttgcggaag acctcaatgc tgatgctccg
     7381 gggaatgcca ctgttcacag caccgacaac gcggagacaa agacactttc tgaacctgcc
     7441 gggatgcacc ctgttcaacg ggcaacccct gaaccaacgg aagaaaataa acctcaggaa
     7501 gagggcgtta tgtctgccac tgatacaggt gaagagagtg ccgctgccgc tatcgatgac
     7561 gacctgaaca cagttgcgga agacctcaat gctgatgtta ctgtgatgac cacagctctc
     7621 cacaccgata cagcagaaac agcctcacct tctgaaccag cagaaattca gcccgtacag
     7681 caggcaaccc ctgagccaac agcagaagat aaaccgaagg aagagcgtcc tgtgtctgcc
     7741 actgagacca gcacagagaa tgaagcagcc cgccctgaaa caccatcgct tttcagccgc
     7801 ctgcgccgtt ttctgaccgg tggaaaatca cgtaccgccg aacctcccgt tattcaggag
     7861 aacacgctta cggaggccga aactgcgaca gaggccgcag caccaccgtc accacctgaa
     7921 accagcccgg aagacacatc gtcagcagaa ccggacacga ttatctttgc gccccgccgg
     7981 cccaacagtc cgcagcggca caacctggac gagattatta aaaatcttac atacgatgag
     8041 ttacccgaca atactgcgct ctgccgcgtc gacggcgaac cggcattcct ggataaactc
     8101 aaccgtctgg aaatggtgga tggtgccagt gctgatgaca aaaaggtcct ggctgccctt
     8161 gctgtggcca ccaacttcta tggcggcgtt atcgagctga ccggcagtga tgcctttaaa
     8221 caaaaagcca tgcagctgat tgtcgaatat gacatcaagg ttcgcatgaa actcccggtc
     8281 cagagggctg cgctggaaaa actccgccag gagaaaggga tagcgcatga tgccatcgtc
     8341 acccacagac ccacgccgga actcaaccgc ctgtcaccgg aagagacaac aatggcacaa
     8401 ccggcccccc ctgtgtcacc gaaagcagaa acagagcaaa ctgcaggcag tacggctgaa
     8461 acaccgcatc cggaagcgcc ggtcacggca aaagctgaag ccccccgagc cggcacaccg
     8521 gaacaacccg ccgcctcgtc gtctgcgcca gaaaaagccc ctgtcagtgc acagccgcag
     8581 caacccgaca caccggagga agagccgccc ggcaagctca gacccggtga aagtgtgact
     8641 gccgtcctga caaactacgg agaaaaagag tatgcgcccg gaaaagggcg ctgctttttt
     8701 gtggagctca ccaaccgcag tggcaaacgg gaatactggg gaaacggcct ggaagaactg
     8761 gtcaaaaacc accagaaagg caaccccgtc acactgacgc tgcaggcgag agaaacattc
     8821 cggctgtccg gtgaaacacg ggatcgcatc cgtaatatct gggccatgac tcctgtcagt
     8881 aacggcatta ccgtcgataa tgaccatcct catgctggtc agcatatcct ggaatttccg
     8941 atgaaaacat tcagtcaggt aatgtcacaa atgcaacaac actggccaca gataatgcag
     9001 gaagttcgct cccccaaaaa tcctcccaat aatctgtaca ttggtgaaga caggcaacct
     9061 gcatcagcac ctcaggatca atcaatgcga ctccccctcg ctggtaatgc ccccaatgaa
     9121 ctgataccag ccatcgcctc tgccgataag gacaatcgtc agttgaatct tctgctggtc
     9181 cacagcgcag atgatcatct gcagggcgtg gtccgcctga acggcaccct ttacccggca
     9241 ctggccaccc cgtcagctga caaccgtcag ctggtcatca atgccctgac ggataacggt
     9301 ctgcagtttg ccggttacgg tgaagctgtt aaccatgatg agaatactca tcaacgtccg
     9361 tccccacaaa taatgcagtt tcacctgaag caacaggact ccccgttgtt tgccgccatc
     9421 cacaaaccgg aagagcaacc ggacaagctt ttccgctctc tgggttttga gcagacctgg
     9481 aaagagtggt ctgacagcca aaaggcggag gacaggcagg aaaaaactct gcaacaggca
     9541 caatcccaca gccccggcct gtgagccggt aaggacgacc atgaacatga aaaaaacgct
     9601 gctggtcata ctgaccagca tctcgctggc ttctccggct gccgtgacag caacctgtcg
     9661 ggcaggtgct gccgcagaag ccggcagcaa agccgcctgg gagcgggcgc gcacagccag
     9721 ccaggcatgg tcacagcgag aaaacgacgt cgcctcctcc ctgcagtcct gcctgtcccg
     9781 cattaaggac gtcagcatca ctcttccaca attccccagc ctggacaaaa tccttcgcga
     9841 tctggaaaac caggtctgtg atgccgtcgt ggatcgggtg aatgaacacc tgccggggaa
     9901 tatcgacccc tggaaagact ataacctctg aacaggagcc ccatgagcga agcacacccc
     9961 acatcccctg tctctccgga aacagataac agcggagata tttataaaca aacgctcagt
    10021 accattgcac tgtcagaaca acgggccgca ctggttcccg gactggtcag ggccttgctg
    10081 tgtagcagcg cagctctcgt catcagtctg accggcaata tcatacagta ctggcataac
    10141 agcaacgtaa cgcgtgaata ctttgccacc gacaatggcc ggctggtccg cctggccccc
    10201 accagtcagc cagcctggag tcagagcgac gtcatgacgt ttggcagcat gacgctgagc
    10261 gaagctttta atctggattt tgtccactac aggaaacaaa tcacgacact ggccccccgc
    10321 ttttccgaag ccggttttgc cggctatgtg aatgctcttc aggcctccaa cattctggac
    10381 accattaaaa aggagaaatt taacctcacg gccaccatcg gggccggcac gctggtgaat
    10441 cagggccaga tggaaaacgg catctggttc tggaaattcc agtatccggt caggatgcgt
    10501 ctggtcggtc agaccagcac ccgccccgag caggccttca cctttgaaat cactgtccgg
    10561 cgcgttgacc cacgaatcaa acccggcggc atggaaatca gccagatggt ttcccgtaac
    10621 gccgcatcag gaacctgaga ctcatatcat gcaaatacat catcttcttg gcgcactgct
    10681 tattgtgtgc ggcactgtca cggctgcaga taacccgggc tggcagaacg cacgaacccc
    10741 acaggctggc ggaaagacag gacaggctcc cgggaacgcc cccgcaggcg
//
LOCUS       DAZFKF010000141        10731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFKF010000141 DAZFKF010000000
VERSION     DAZFKF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.143"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(273..1970)
                     /gene="treA"
                     /locus_tag="ACHKA1_004637"
     CDS             complement(273..1970)
                     /gene="treA"
                     /locus_tag="ACHKA1_004637"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO5682192.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPT
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHVPNGNRSYYLSRSQPPFFAMMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGIENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQREVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSELTTQPVKQKEAEPTP"
     gene            complement(2290..3708)
                     /gene="dhaM"
                     /locus_tag="ACHKA1_004638"
     CDS             complement(2290..3708)
                     /gene="dhaM"
                     /locus_tag="ACHKA1_004638"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO5682193.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSAVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNHQS"
     gene            complement(3719..4351)
                     /gene="dhaL"
                     /locus_tag="ACHKA1_004639"
     CDS             complement(3719..4351)
                     /gene="dhaL"
                     /locus_tag="ACHKA1_004639"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO5682194.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGNAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4362..5432)
                     /gene="dhaK"
                     /locus_tag="ACHKA1_004640"
     CDS             complement(4362..5432)
                     /gene="dhaK"
                     /locus_tag="ACHKA1_004640"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO5682195.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFVTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            5660..7579
                     /gene="dhaR"
                     /locus_tag="ACHKA1_004641"
     CDS             5660..7579
                     /gene="dhaR"
                     /locus_tag="ACHKA1_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO5682196.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     HDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAVL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(7679..10546)
                     /locus_tag="ACHKA1_004642"
     CDS             complement(7679..10546)
                     /locus_tag="ACHKA1_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5682197.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVASGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVVMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTIPEPT
                     PNPEPTPAPAQPPIVNPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVLDSQNNG
                     VNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGKSQLNIYVKTGAIHEFSGDTEYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
BASE COUNT         2566 a         2650 c         2690 g         2825 t
ORIGIN      
        1 tgtttgatgt caaaatttgt ctaaagagac agaatgattt gcttgttgct taaataacac
       61 tttgttccac aagaaaattg tcagggagaa tggcggagtg gcgaaaaata taaatagagg
      121 tgtactgatt aacaaattga taaaataaaa ttaaaatctt ctctgatata agaaaataaa
      181 ggcatagacc gtagaatggg gggcatttcg tggcgaaaaa agagcaatgc caattttatg
      241 cacataatgg cattgctcga tatacagcaa agttaaggcg taggttccgc ctctttttgt
      301 tttaccggct gtgttgttaa ttcactgagt ggacgcgtcg ccggaacatt gtcacacggt
      361 tgctctttcg ggcagatcaa atccagcatt ttcagcgtca cgccattggt ccagccaaag
      421 ccatcctgta atggatattc gccaccgccg ccccccgttc cggtggtgct gacatcatat
      481 ttttccacca gctttttctc ccggtcatag gtgtgctgaa cattggtcag gaagtgccag
      541 ctaatgtcca tcgccacctc tctttgcccg tagttttgta atccttctgt cgcgacccac
      601 tgtaacggtg cccagccatt tggcgcatcc cattgttgcc cacttttcac cgacgtggtg
      661 ttcaggccgc cgggttgcag cagatgtgtt ttcgtcgccg tcgccatttt gttggcgcga
      721 tctttcgctg ccgcattgac gtacagcggg aacagggcgg ccgcggttaa ctgattgcgc
      781 actttatgac ttttcaggtc gtaatcggca taccagcctt gttgatcgtt ccacaggtat
      841 ttttcgatcc ctttttgacg ggcatttgcc agcgtttcgt actggtttgc catcgcgtta
      901 tctccggcag ctttgctggc gcgggcgagg attttttcca ttttaaacat caggctgttc
      961 agatcgaccg gtacgatgct ggtggtgcgt aaggtattta actgctgcgg gttgtccatc
     1021 cagcgcgagc tgaaatccca gccagacgca gcggcagagc gcaggtcgcg gtaaatttca
     1081 gtggcaggtc gattcggatt gcttttggcg gtggcaatat cttccaccca tgactctggt
     1141 cgtggcgtat cgcgatcatc ccagtagcgg ttgagaaggg taccatcctg aagttttaca
     1201 acgcgttttt cctgttgtcc ggcttgcagg ttttcaatac cgtccatcca gtaagcatat
     1261 tctttttgca tttgcggcag gtattgcttc aatgcagcat cgccttcatg ctgcgccagt
     1321 aactctacca tcatggcaaa gaagggcggc tgcgagcggc ttaaatagta actgcggttg
     1381 ccgttgggaa catgaccgta agtgtctatt tcatgagcaa aattggccac catatccgcg
     1441 accttatccc agtgaccgct ttcggcaagt cctaacatgg tgaagtaact gtcccagtaa
     1501 tatacctcgc gaaaacgtcc gcctggcacg acataaggtt ccggcagcgg taacagagaa
     1561 tcccattttt cggtgttttc ggtagaacgc gttaataccg gccaaagtcc gtcaatatgt
     1621 tcgcgcagtg actgtccctc tggcggaaca tatttctcgc cctctgtcgg cagggtgaaa
     1681 ttgacgttaa cgaaatggcg cagatcaaat ccgctctggt tttgctgcat ccgataatca
     1741 gcaaggatca tcagcggatc gctgttcggc acggcatcgg caaaggtttt ttggtccgga
     1801 aaaagtttgg cgttttgcac atcattaaac agcggcccta ataaaatatc aggcggctgt
     1861 ggcgttaccg gtgtttcttc tgcctgcacc gatagcgcag cgaaacacaa aaagatgcag
     1921 gctggaatta acgccatttt ttgcgggcga gaaggtgcgg gggatttcat caatcattct
     1981 ccttaggcga aaccgaataa aacggtattc aagtatcaga aaaccttagt tcaggatcgc
     2041 actagctgca tgatcgaaat ctcattctgc gaactgtcag gcattttaga aaagggaatt
     2101 aaagggataa ataacggcga aatagagtga tcaaataacg tctgtttatt agccagccag
     2161 cggcgtttgg ttcgcgatta gcgaagaaaa atcatcataa attggtgaaa aatataacag
     2221 gtagttaaaa aacattagtg ctgagtaaat tgccggatga catcagaacg atgccatccg
     2281 aacggtggtt taactctgat ggttgaaacg ttgcgtttta acgtccagcg ttagcgtttc
     2341 ttctggttgt atcgcataca gtttctcacc ctgctggcaa atccagccaa tccccagttc
     2401 acgggcgatc agggcgctgt gggataccgg acttccggcg ctaaggcaga taccttttac
     2461 aaccgccgga tccagttgca gtactgcgga gggataaatg ttctccgcca gtagaatagt
     2521 tggcgagtta aactgcggga gttcttcttt cgtttgggtc aggtggacca gggtgcgatg
     2581 cagaagatcg tccacatcaa tatagcgagc ttgtagatat tcatcatcca gttgctggta
     2641 ttgctggcta agttctttaa gaacttgctg ccaggcatat tctgccgtgc aatgttcgtg
     2701 ctgaaggagt tcgcttgccg ccgccagcag ttccggatca tctaacagtg tatggtgacc
     2761 agaaaagatt gcggcaatat cgtcaagccc gctggcttct gctttcgctg ttaacgtcat
     2821 cagatctaat aacgtgaagt caatggcctg gcgtaatcga tcttgttcct cttccacggt
     2881 cagggttgat ttggcctgta ccgtacataa aactggttga taataaaatg ctttacccga
     2941 aacaggcgga acgggacgca gagtaggtgg agcaacttcc tccgtttcac caaagttatc
     3001 ttcagccagc tgacggaaag cgatcagtgc ctcttcagct tctggccctt tcgcaatcag
     3061 gcgcagcgta tcgttatagc gaacttgtag taacgcaatc tggttaatac tctccggtgt
     3121 gacgcatttg ccgtttttct ccagcaacat atcggcatta aatgtcgata aggtataaac
     3181 cagccgggag gctggacgta catgcaggcc gttacggttc tttatgacca ccgccagaga
     3241 acgggcttct tcatcgtacg gaggacatgt gtcagagatt tcagtgtcgg aggacggtaa
     3301 acccagttgt tcacgtttgg cttccagcgc atgcatggcg tcaaagataa ctttgtcgat
     3361 atccgccccc gaggccgcgc tgaccgttgc tgccagtgta ccttcgacca acggtgcagc
     3421 acacaaacgt acttttgcag cgatctcggg agccagcaat tccagcgcag tttcagcact
     3481 caataatgcg ctacccatat ccatcatgac cagcacatgg tcggcatcgg caacagattc
     3541 gatggcctcc atcactttga cggcatcggt accaatggga ttttgtggat cgtcaattcc
     3601 cgcggcaatg gcgattttac aactatcact cattaacatc tgacgggcta attcaccgac
     3661 accttctccc agtcggctgc tatgtgaaac tatgaccagg tttaccatca ccaattcctt
     3721 actcttttgc ggctaacgcc aacatttgca tcataaacat caccgaggtt gcgccgggat
     3781 cctggtgacc aatactgcgt tcaccgagat aactggcgcg gcctttgcgg gcttgcatcg
     3841 taatcgtact ttgtgcagcg gattcggcga tgctactggc agcttcgagt gccgccggaa
     3901 cagagagatt ttgctcgctg gactgacgta acgattccac caccggcacc cacacatcac
     3961 acatggtttt atcgccaggt tcggcgttcc cgcgactgat tacgccgtcc gcgccatcgc
     4021 ggaacatctg ataaagctct tccagcgtca ggctttgccg tgcctgggtc gcctgtgcgg
     4081 cgcgtataaa gaaggtaccg aatagcggac cactggcacc accgacgctg gaaagcagcg
     4141 tcataccggt attcttgaga atgaaaccga tatctttatc tgcgatagca gggagttttt
     4201 ccaccacttt gctaaagcct cggttcatat ttagcccgtg gtcagcgtcg ccaatctcgc
     4261 gatccagtcc ggtaagatac tcgctctcgg tgttgaaaat atcgccacaa cgagtgagcc
     4321 agttaacaat ttgagttctg ctcagtgaca ttgctttctc cttatttacc ccagttaagg
     4381 gccggggtgt ggaccggggc gtcccagagt gccagcgttt cgtcatcaac tttcagtaag
     4441 gtgattgaga aaccggtcat atccagtgag gtgcagtacg cgccaattaa attacgctcg
     4501 atagtcaatc ccgcttgctg gcaacgtgtg gtcaggcggt tatagacgcc gtacagctca
     4561 gaaagtggag ttgcgccaag attgttaacc agcgcaatca ccctgtcgcc agactggagc
     4621 ggttgtttgg tttgtggttc ttcctgccag ctgccttgtt gataatccca gaaacgcaaa
     4681 gtgcgatggt atgagccatt ttccagcagg gtgacgaaca tttcatcgac ggtttgatca
     4741 agggaagaga aggggcgacg gtcaataccc ggctcaccat gaatgccgac gccaaactcc
     4801 atctcattgt ccgccagggt aaaagaaggt ttgcccgcgg caggaacggt acaggcaccg
     4861 agagcgatac ctattgagtg gccttgatta ttcaacttac gccccagttc cgcacaggcg
     4921 tccagtgagt cgccacgctc cgccgctgcg cctacgagtt tttcaattaa tacggtgttg
     4981 gcaacgccgc gccgcccggc ggtataaaga ctgtctttta ctgcaacgtc gtcatcaatg
     5041 accacagtgg tcacttttac gccgctatcg tgcagtaact cggtcgctgt ttcaaagtta
     5101 agaatatcgc cggtgtaatt tttgataatc aacagtacac cttcgccgcc atcaacttgc
     5161 atggcgcatt caaagatttt atcgggcgtc ggtgaggtga aaatttcgcc cggacaggcc
     5221 cccgaaagca tcccctgacc gatataacca cagtgcatcg gctcgtgtcc gctgccgcca
     5281 cccgacagca gggcgacttt tcctgcaaca ggggcatcag ctcgggtgac atacaccgga
     5341 tcctgatgca gtgtcagcga tggatgcgct ttcgccagtc ctgccagttg ttcgtccagt
     5401 acgtcttgca catcattgat caattttttc attattttgc tccagcaatt acggtagggc
     5461 atggatgatg ttcaacgaca cggcgaccac tgactgccga tgaatccatt gtgcatcagg
     5521 caagggaaaa gaaaattagc ccaaattatg tttcatagtg aaacatatgc tttaatgaat
     5581 gttccatatt gaaactttta cgtgtattaa tacttaaaat tgagagccgg aacacctttt
     5641 gtcataaggg atgcgggata tgagtggcgc ttttaacaac gatggtcggg gcatatctcc
     5701 cttaattgca acctcctggg agcgatgcaa taagctgatg aaacgggaga catggaacgt
     5761 accacatcag gcccagggcg tgacatttgc ttctatttat cggcgtaaga aagcgatgct
     5821 gacgctcggg caggctgcgc tggaagatgc ctgggaatat atggcaccgc gagagtgtgc
     5881 gctgtttatt ctcgatgaaa ccgcctgcat tctcagccgt aatggcgatc cgcaaacctt
     5941 gcagcagcta agtgcacttg gattccatga cggcacgtat tgcgccgagg gaattattgg
     6001 tacttgtgcg ctatcgttag cggctatctc tggtcaggcc gtgaaaacga tggccgatca
     6061 acatttcaaa caggcactct ggaactgggc cttttgtgca acgccgttgt ttgacagcaa
     6121 gggccgattg acgggaacaa tagcgctggc gtgtccggtt gagcaaacta ccgcagctga
     6181 tttgccgttg acgttggcaa tcgcccgcga ggtcggtaat ttactgctga cggacagttt
     6241 gctcgctgaa actaaccgtc atttaaatca acttaatgcc ctgttagaaa gtatggatga
     6301 tggcgtgatt agctgggacg agcagggtaa tttgcaattt attaatgccc aggcggcgcg
     6361 ggtcttgcgc cttgacgcga cggcaagtca gggacgggca atcactgaac tcttaacgtt
     6421 acccgccgta ttgcaacaag caataaaaca ggcacatccg ctcaaacacg tagaagcaac
     6481 ctttgaaagc cagcaccagt ttattgatgc ggtgataacc cttaaaccga taatagaaac
     6541 gcagggaacc agctttattt tgttgctcca tcctgtggaa cagatgcggc agttaatgac
     6601 cagtcaatta ggaaaagtca gccatacctt cgctcatatg ccacaggacg atccgcaaac
     6661 ccgccgcttg attcattttg gtcgccaggc ggcgcgcagt agctttcctg tcctgctttg
     6721 tggagaagaa ggcgtgggca aggcactgct aagtcaggca attcataatg aaagcgagcg
     6781 tgctgcaggt ccttatatcg ccgtcaattg tgagttatat ggtgatgcgg tgctggcgga
     6841 agaatttatt ggcggcgatc gcacggacaa tgaaaatggc cgtctgagtc ggctggaact
     6901 ggcgcacggc ggcacgctgt ttcttgaaaa gattgaatat ctggcggtgg agttacagtc
     6961 tgctttgctt caggttatca agcagggtgt tatcacgcga ctggatgcgc ggcgtttaat
     7021 accaattgat gtcaaagtga ttgctacaac gaccgcggac ctcgcaatgc tggtggaaca
     7081 aaatcgtttt agtcgccagc tgtattacgc gctgcatgca tttgaaatta ccatcccgcc
     7141 tctgcgtatg cggcgtggca gcattccggc tctggtgaat aacaaattac gcagtcttga
     7201 aaaacgcttc tctacgcggc tgaaaattga tgacgatgcc ctcgctcgcc tggtttcctg
     7261 tgcatggcct ggcaacgatt ttgaacttta cagcgtcatc gagaatcttg ctctgagtag
     7321 tgataatggg cgcattcgcg tcagtgattt gccggaacat ctgtttaccg agcaggcaac
     7381 agatgatgtc agcgccacac gcctttccac cagtctgtca tttgcggaag ttgaaaaaga
     7441 ggcaattatt aacgcagccc aggtcacagg cggtcgcatt caggaaatgt cggctttact
     7501 tggaatcggc cgcactacgc tgtggcggaa aatgaagcaa catggcattg atgcagggca
     7561 gtttaagcgc cgggtatgaa agacagaaac gatttctgat acatcagagt gatctatatt
     7621 tcattccggc gcacgctaac atttttcagc atcgtttaag ggcttgtcta tcccgcactt
     7681 aaaagctgaa gcgatatcct ccgttgactt gcttctgatc aaagaggtta ccctgcgtgt
     7741 aatccgcttc gaggtagaat gtgtgctgtt tgttatactg tgcactgact cccacgccgt
     7801 tattccagcc attacctttg aagctgtact tctcccggga gttgttcaac agatattctg
     7861 tatcccctga aaactcgtgg atagcgccag tcttcacata gatattcagc tggcttttac
     7921 ctgcggtgat gtcatatccc agtatcatgc tggcacgccc cagcagcgat tcgtagtgat
     7981 tcagatgtat attgaggcca ttactcgcct tcatagccat ctcattctgg tggctgtatg
     8041 tgagctgggt ttgcggctct atatagaacc cataaccagt aggggtcagg ttgaacctct
     8101 gcccggcttc cagggagatg ctcaatccat tcgcagtgcc gttggcgtta acgccgttgt
     8161 tctgactgtc cagtacgtgg aaactatttt tctggcgcga tgcttttaca acgagatcgc
     8221 tgtaaaaacc gttgtgtgcc atgtaactgg cgtacattcc catgtagtct gaacgtgcgg
     8281 taccgtcgcc accgctatag tccggcgatg catgtgttga gccaatatac agaccgacat
     8341 acaacggcat tacatcagag agacgtttat ccccaccaaa ctggataccg ctgtaaccca
     8401 tgtcaaagcc gctcagtttg ccactggcga aggagtccag gcttccccca taactgcgaa
     8461 gccagatatt accgtcttta ctctgatttc gcaggtcacc cattcgttgc atcagcgtac
     8521 ggttttcaac atagtttaat aagtaaccga cattgagata attgccgcca gcatctgcgg
     8581 tcgtggtggg tttaggtgtg ggagtggggt taggcgttgg atcggggttg actatgggag
     8641 gctgagcggg agccggcgtt ggttcaggat ttggagtcgg ctccggaatt gtcccggaag
     8701 cataaagctc ccagttagtg ccatttttac gcacatcgta cagatatccc cccaactcaa
     8761 cctgagaaga cgcgctgaac gaggccgcac cgtcagtggt ttttaccact gtcagaactt
     8821 catttcccgt tgtggcctcg ctgccctggt tgcggatagc caatacatga ttaccagcac
     8881 tactcccgct gatattcaat aaatcccctt tattattaac gccattaccc tcgccaacaa
     8941 catcagcacg cataataaag gtactgttac cgctcaggtt ctctacgttt aatgtggtaa
     9001 atgtgccggc agttgacccg tggctggcaa aatcgacagt tgaatggctc agcgccagcg
     9061 tgtcgagatt agaattactt gttacgttcc agacgctgtt attcattaca acgtccagtt
     9121 tcccgccatt gacattatcg ctgagggagg aacctgtcca aaccgaccca gggtgcatat
     9181 ccagattgat tagcccacct ttggaaagaa cgctaccgtt gataagcata cgacccgagg
     9241 cgttgatgcg gctggcggca taaccatcgt catgttgcgt tgcgatggcc atctggtcgg
     9301 gtgtactcat atcaatgacc agatcgctcg tgaggtcgat ctggctgttg gtcatggcat
     9361 aaatccccct ggctcctgcc gcgccggtga tagccaaact gtctccggtt attctaccgc
     9421 cgctgagcgc ccacaaaccc agcgccagac tgccattacg atcaaccgta atatccgtat
     9481 tttgcatgtt gataacagcc gttgccgtct gggccgaggc accataagag ccgccggaaa
     9541 agatgctgtt tcgttgcgct gccgtgccag aaaaattgat tgtcgcgtct gaaccactgg
     9601 ctacgagacc accgccctgc gcggaagaaa tatggctatc tgcaccgata gtggttgtac
     9661 caccacgaac ttcgacgcca ttggccgcgg ctccagttac atcaacagtg agtgcattcg
     9721 cgctaacctg gccaaagctc cagaggccgt gagcattatc gccattggtt ttaatggtac
     9781 tgtttgttcc gagatcgaca acagagtttt tctgtacgtt tatccccatg gcgctgtagc
     9841 cctgaacatc gattgtcagg tctgtcgccg taaaacgcgc agcaccattg gcgttattgc
     9901 cgttgagacc accgatataa acacctgtac ttccatcggt cttgatttta cttccgcttc
     9961 caagatcgac actggtgcca tagtcattga tggttaggcc tataccgttc gagttttcaa
    10021 tggtgaattg agtggctgtt agtgttgagc tatgcccaat aatgatgccg tcatcgttac
    10081 tcttaatggt gctgcctgtg cctaagtcag catgggtata gtcaccaatt aaattaatgc
    10141 cgatggcaga ggtttgacca acaacatcta ctgtgagtcg gttagctgtc agggagctgt
    10201 ttttgccctg aataatgatt ccttttgcat atccagaggc gtcgttgaca ttgacggtga
    10261 catcattacc caggttgata ttgccaggcg tgtttcctgc tggggtcatt acaccataga
    10321 ggtttcctga tggatcggcg gtatctcctg taattctgtc accatcatta atagtaatat
    10381 tggtgccata ttgacttcct gtatcagcag ctatcagcag gggggaaaag ataattgcat
    10441 tcaaaccaat agcgccaaat tttatcagtt gaattgaggg cgaacggatt tttaattcag
    10501 cgagacgatc ggctttggta ttcccattgt gttgtttgat gcccatacca ctcctatata
    10561 gtacccaggc tatgggtcta tggattttgt cattttgttt aagtcaatga ataatatcct
    10621 cttatcatca tgatatttgt atggtgcaat catgatgagc tgctatgaga ataattctct
    10681 attaccgggc aaaatcagaa acacaggaat taatatatat ttaacatccc c
//
LOCUS       DAZFKF010000142        10617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFKF010000142 DAZFKF010000000
VERSION     DAZFKF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.144"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..79)
                     /gene="yojO"
                     /locus_tag="ACHKA1_004643"
     CDS             complement(<1..79)
                     /gene="yojO"
                     /locus_tag="ACHKA1_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO5682198.1"
                     /translation="MHTPIEVKPVAGSKEWREAWQKRAFA"
     gene            188..682
                     /gene="napF"
                     /locus_tag="ACHKA1_004644"
     CDS             188..682
                     /gene="napF"
                     /locus_tag="ACHKA1_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO5682199.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            672..935
                     /gene="napD"
                     /locus_tag="ACHKA1_004645"
     CDS             672..935
                     /gene="napD"
                     /locus_tag="ACHKA1_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO5682200.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNASPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            932..3418
                     /gene="napA"
                     /locus_tag="ACHKA1_004646"
     CDS             932..3418
                     /gene="napA"
                     /locus_tag="ACHKA1_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO5682201.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3425..4120
                     /gene="napG"
                     /locus_tag="ACHKA1_004647"
     CDS             3425..4120
                     /gene="napG"
                     /locus_tag="ACHKA1_004647"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO5682202.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4107..4970
                     /gene="napH"
                     /locus_tag="ACHKA1_004648"
     CDS             4107..4970
                     /gene="napH"
                     /locus_tag="ACHKA1_004648"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO5682203.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4967..5416
                     /gene="napB"
                     /locus_tag="ACHKA1_004649"
     CDS             4967..5416
                     /gene="napB"
                     /locus_tag="ACHKA1_004649"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO5682204.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5426..6028
                     /gene="napC"
                     /locus_tag="ACHKA1_004650"
     CDS             5426..6028
                     /gene="napC"
                     /locus_tag="ACHKA1_004650"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO5682205.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6041..6664
                     /gene="ccmA"
                     /locus_tag="ACHKA1_004651"
     CDS             6041..6664
                     /gene="ccmA"
                     /locus_tag="ACHKA1_004651"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO5682206.1"
                     /translation="MGMLEVRELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAV"
     gene            6661..7323
                     /gene="ccmB"
                     /locus_tag="ACHKA1_004652"
     CDS             6661..7323
                     /gene="ccmB"
                     /locus_tag="ACHKA1_004652"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO5682207.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7365..8102
                     /gene="ccmC"
                     /locus_tag="ACHKA1_004653"
     CDS             7365..8102
                     /gene="ccmC"
                     /locus_tag="ACHKA1_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO5682208.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8099..8308
                     /gene="ccmD"
                     /locus_tag="ACHKA1_004654"
     CDS             8099..8308
                     /gene="ccmD"
                     /locus_tag="ACHKA1_004654"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO5682209.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRSVAQQRAREARLRAAQQQEAA"
     gene            8305..8784
                     /gene="ccmE"
                     /locus_tag="ACHKA1_004655"
     CDS             8305..8784
                     /gene="ccmE"
                     /locus_tag="ACHKA1_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO5682210.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8781..>10617
                     /gene="ccmF"
                     /locus_tag="ACHKA1_004656"
     CDS             8781..>10617
                     /gene="ccmF"
                     /locus_tag="ACHKA1_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO5682211.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRW"
BASE COUNT         2165 a         2730 c         3194 g         2528 t
ORIGIN      
        1 gagcaaaagc ccgtttttgc cacgcttccc gccactcttt tgatcctgct acaggtttta
       61 cctcgatcgg ggtatgcatc tttgacacat cctttaatat cttagcggct ataaaaatgg
      121 cttattaatt atgcggttta tttggtcgct ctcaattttc agagcgcgtt aatgatggaa
      181 ggtcaatgtg aagattgatg catcccgtcg gggcatactc actggtcgct ggcgcaaagc
      241 cagtaacggt atccgtccgc cctggtccgg tgatgaatct cattttctga cccattgcac
      301 ccgctgcgac gcctgtatta atgcttgtga aaacaacatt ctgcaacgcg gcgcgggcgg
      361 ctatccgagc gttaatttca aaaataacga gtgcagcttc tgttacgcct gcgcgcaggc
      421 ttgccccgaa tcgctatttt ctccgcgcca caccagggcc tgggatttgc agttcaccat
      481 tggggacgcg tgcctggcgt atcagtcagt tgaatgccgc cgctgtcagg atagctgtga
      541 gccaatggca attatctttc gcccgacgct ctccgggatc taccagccgc aacttaatag
      601 ccaactttgc aacggatgtg gagcatgtgc agccagctgt ccggtatcag ccataactgc
      661 ggagtatctc catgcacact aactggcaag tttgcagcct ggtcgtgcag gccaaaagcg
      721 aacgaatttc agacatcagc acccaactga acgcctctcc cggctgtgaa gttgctgtca
      781 gcgacgcgcc gagcggtcag ttgattgtgg tggtggaagc agaagacagc gaaacgctga
      841 tccaaaccat tgagtcagta cgcaacgtag agggcgtgct ggcggtgtcg ctggtttatc
      901 accagcagga agagcaaggt gaggaaacac catgaaactc agtcgtcgta gctttatgaa
      961 agctaacgcc gttgcggccg ctgcggcggc tgccggtctc agcgtgccgg gcgttgcccg
     1021 cgccgttgtt ggtcagcagg aagccatcaa atgggataaa gcgccgtgcc gtttctgcgg
     1081 tactggttgc ggcgttctgg tcggaacgca gcaggggcgt gtggtggcct gtcagggcga
     1141 cccggacgca ccggttaacc gtggcctgaa ctgcattaag ggctatttcc tgcccaagat
     1201 catgtacggt aaagaccgtt tgacgcagcc gctgctgcgt atgaaaaacg gtaaatatga
     1261 caaagaaggc gaatttaccc caatcacctg ggatcaggcc ttcgatgtga tggaagagaa
     1321 attcaaaacc gccctgaaag aaaaagggcc ggaatcgatc ggtatgttcg gttctggtca
     1381 gtggactatc tgggaaggtt atgccgcatc caagctgttt aaagcgggct tccgttcgaa
     1441 caacatcgac ccgaacgcgc gtcactgtat ggcgtcggca gtagttggct ttatgcgtac
     1501 ctttggtatg gatgagccga tgggctgcta tgacgacatc gagcaggctg acgcgtttgt
     1561 gctgtggggc gcaaacatgg cggagatgca cccgatcctc tggtcacgca tcactaaccg
     1621 tcgtctctct aaccaggacg tcaccgtggc ggtgctttca acctaccagc atcgtagctt
     1681 cgagctggcg gataacggca tcatctttac gccgcaatct gacctggtga tcctgaacta
     1741 catcgccaac tatatcattc aaaacaatgc gataaatcag gacttcttta gcaagcacgt
     1801 taacctgcgc aaaggggcga cggacatcgg ctacggttta cgtccgaccc atccgctgga
     1861 aaaagcagcg aagaatccgg gttctgacgc ctccgaaccg atgagctttg aagattacaa
     1921 agccttcgtt gctgagtata cgctggaaaa aaccgctgaa atgactggcg taccaaaaga
     1981 tcagctggaa caactggcgc agctgtatgc cgatccgaac aagaaagtca tctcctactg
     2041 gacgatgggc ttcaaccagc atactcgtgg cgtgtgggcc aacaacctgg tctacaacct
     2101 gcacctgctg accggcaaaa tttcccagcc gggttgcggt ccgttctccc tgaccgggca
     2161 gccttccgcg tgtggtactg cgcgtgaagt gggcaccttt gctcaccgtc tgcctgcgga
     2221 catggtggta actaacgaga aacatcgtga tatctgcgag aagaagtgga atatcccgag
     2281 cggcaccatt ccggcgaaaa tcggtctgca tgcggtagca caagaccgtg cgctgaaaga
     2341 cggcaagctg aatgtttact ggaccatgtg taccaacaac atgcaggccg ggccgaacat
     2401 taatgaagag cgtatgccgg gctggcgcga tccgcgcaac ttcatcatcg tctccgatcc
     2461 gtatccgaca gtcagtgcgc tggccgccga cttgatcctg ccgaccgcaa tgtgggtaga
     2521 aaaagagggg gcttacggta acgccgaacg ccgtactcag ttctggcgtc agcaggtaca
     2581 ggcgccaggc gaagcgaaat cggacctctg gcagttagtc cagttctccc gccgcttcaa
     2641 aactgaagaa gtatggccgg aagagctgct ggcgaagaaa ccggaactgc gtggcaaaac
     2701 gctgtacgaa gttctgtatg ccacccccga agtgagcaaa ttcccggtat ccgaactggc
     2761 ggaagatcag ctgaacgatg aatcccgcga gctgggcttc tatctgcaaa aagggctgtt
     2821 cgaagagtac gcatggtttg gtcgcggtca cggtcacgat ctcgcgccgt tcgatgacta
     2881 ccacaaagcg cgcggtctgc gctggccggt ggtgaacggt aaagaaacgc agtggcgtta
     2941 cagcgaaggt aacgacccgt acgtgaaagc gggcgaaggc tacaagttct acggtaaacc
     3001 ggatggcaaa gcggtgatct tcgcactgcc gttcgaacca gcggcggaag caccggatga
     3061 agagtacgac ctgtggctct ctaccggacg cgtcctggag cactggcaca ccggcagtat
     3121 gactcgccgt gtgccggaac tgcaccgtgc cttcccggaa gcggtcctgt ttattcaccc
     3181 gctggatgcg aaagcgcgcg atctgcgccg tggcgacaaa gtgaaagtgg tttctcgccg
     3241 tggcgaagtg atctcgattg tcgaaacgcg cggtcgtaac cgtccgccgc agggcctggt
     3301 gtacatgccg ttcttcgacg ccgcacagct ggttaacaaa ctaacgctgg acgcgaccga
     3361 tccgctctcg aaagagacgg acttcaagaa gtgcgcggtc aaactggaga aggtgtaagc
     3421 cattatgtcc cggtcagcga aacctcaaaa tggtcgccgc cgctttctgc gcgatgtcgt
     3481 tcgcacagca ggcgggctgg ctgccgtggg tgtggcgctg gggttacaac agcaaaccgc
     3541 acgcgcatct ggcgtgcggt tgcgcccgcc cggagccata aacgagaacg cctttgccag
     3601 tgcttgtgtg cgttgcggtc agtgtgttca ggcttgccct tacgacacct taaaactggc
     3661 gacgctggcc tctggcctgt cggcgggcac gccatatttt gtcgcacggg atattccttg
     3721 cgaaatgtgt gaggacattc cgtgcgccaa agtgtgcccg agcggtgcgc tggatcgtga
     3781 gattgaatcg atcgacgacg cgcggatggg gctggcggta ctggtggacc aggaaaactg
     3841 tctcaacttt caggggctgc gctgcgatgt ttgttatcgc gaatgcccga aaattgatga
     3901 ggccatcacc ctggagctgg agcgcaacac gcgtaccggt aagcacgccc gctttctgcc
     3961 gacggttcac agcgacgcct gtaccggttg cggtaagtgc gaaaaagtgt gcgtgctgga
     4021 acaaccggca atcaaggtgt taccgctgtc actggcgaaa ggggagttag gccaccatta
     4081 ccgcttcggc tggctggagg ggaacaatgg caaatcgtaa acgtgacgcc gggcgcgagg
     4141 cgctggagaa aaaaggctgg tggcgcagtc accgttggct ggtgttacgt cgcctttgtc
     4201 agttcttcgt gctggggatg tttttgagtg gtccgtggtt tggtgtgtgg atcttgcacg
     4261 gcaactacag cagtagcctg ttattcgaca ccgttccgct gaccgatccg ctgatgacgc
     4321 tgcaaagtct tgccagtggt catctgcctg ccacggtggc gttgaccggt gcggtgatta
     4381 tcaccgtgct ctacgccctg gcaggaaagc gattattttg cagctgggtc tgcccgctga
     4441 acccgattac cgacttagcg aactggttac gcaggcgctt tgacctcaat cagtctgcga
     4501 cgatccctcg ccacattcgc tacgtgctgt tggtggtcat tctggtggga tcagccttaa
     4561 ctggcacgct catctgggaa tggatcaacc ccgtttccct gatggggcgt agcctggtaa
     4621 tgggcttcgg cagcggcgcg ctgcttattc tcgcgctgtt tttatttgat ctactggtcg
     4681 ttgaacacgg ctggtgcggg cacatttgtc cggttggcgc gctgtatggc gtgctgggta
     4741 gcaaaggtgt gattaccgtt gcggccagcg atcggcagaa atgtaaccgc tgtatggatt
     4801 gttttcatgt ttgcccggaa ccgcatgtgc tacgtgcccc ggtgctggat gaacaaagcc
     4861 cggtgcaggt caccagccgc gattgcatga cgtgcggtcg ctgcgtggat gtctgttctg
     4921 aggatgtatt tacaataact acacgatgga gttcgggagc gaaatcatga aaagccatga
     4981 cctgaagaaa gcgctgtgtc aatggacggc gatgctggcc ctggtggtaa gcggcgcggt
     5041 ttgggcggct aatggcgtcg attttagcca gtcgccggaa gtatccggga cgcaggaagg
     5101 ggccattcgg atgccaaaag agcaggatcg gatgccgctg aactatgtga atcagccgcc
     5161 aatgatcccg catagcgttg aaggttatca ggtaacgacc aataccaacc gctgcttgca
     5221 gtgccacggt gtcgaaagct atcgcaccac tggcgcgccg cgtatcagtc ctactcactt
     5281 tatggacagc gacggcaaag tgggcgcgga agtggctccg cgtcgttatt tctgtctgca
     5341 atgtcacgta ccgcaggccg ataccgcgcc aatcgtgggg aataccttta ccccatcaaa
     5401 aggttacggg aaataagagg ttattatggg aaattctgac cgtaagcctg gtctgattaa
     5461 gcgcctgtgg aaatggtggc gtacccccag ccgtctggcg ctggggacgc tgctgttgat
     5521 cggttttgtc ggcggcatcg tcttctgggg cggctttaac accgggatgg aaaaagccaa
     5581 taccgaagag ttctgcatta gctgccacga aatgcgtaac acggtgtatc aggaatacat
     5641 ggattccgtg cactacaaca accgtagcgg cgtccgtgcg acctgtccgg attgtcacgt
     5701 tccgcacgag tttgtgccga agatgatacg caagctcaaa gcaagtaaag agctgtatgg
     5761 taaaattttt ggcgttattg acacgccgca gaaatttgaa gctcatcgtc tgacgatggc
     5821 acagaatgag tggcggcgca tgaaggacaa taactcgcag gagtgccgta actgtcacaa
     5881 cttcgagtat atggatacaa ccgcccagaa atcggttgcc gcgaagatgc atgaccaggc
     5941 ggtgaaagat gggcaaacct gtattgattg ccataaaggg atagcgcaca agctccccga
     6001 tatgcgtgaa gtcgagccag gtttttaaca gggttattgc gtgggtatgc ttgaagtcag
     6061 agagttactt tgtgagcggg atgaacgaac cttatttagt ggcttgtcat ttacgctgaa
     6121 cgcaggagag tgggtacaaa tcaccggtag caacggcgcg gggaagacaa cgcttctccg
     6181 tttgctgacg gggttgtctc gccctgacgc aggcgaggtt ctctggcaag ggcagccctt
     6241 gcatcaggta cgcgacagct accatcaaaa cctgttatgg ataggccatc agccggggat
     6301 caaaacccgg ctgacggcgt tagaaaatct gcacttttat catcgcgatg gcgataccgc
     6361 acaatgtctg gaagccctgg cgcaggccgg gcttgccgga ttcgaagata ttccggtaaa
     6421 tcaactttct gccgggcaac aacgccgcgt cgctttagcg cgtctgtggc tgacccgtgc
     6481 cacgttatgg atcctcgacg agccttttac cgcgattgac gttaacggcg tcgatcgtct
     6541 gacccagcgt atggcgcagc atacagagca gggggggatt gtgattctca ctacccacca
     6601 gccgctcaac gttgctgaaa gtaaaattcg ccgcatttca ctgacgcaaa cgagggccgt
     6661 atgatgttct ggcgcatttt ccgtcttgag ctgcgcgtag cgtttcgcca tagcgctgaa
     6721 atcgccaacc cgctgtggtt cttcctgatt gtaattaccc tttttccgct cagtatcggt
     6781 ccggaaccgc aactgctggc acgtattgca ccgggcatta tctgggttgc cgcgctgctt
     6841 tcatccctgc tggcgctgga acgactgttc cgtgacgatt tgcaggacgg cagtcttgaa
     6901 caattgatgt tgttgccgtt acccttgccc gccgttgtgc tggcgaaggt gatggcgcac
     6961 tggatggtaa ccggtctgcc gttactcatc ctttcgcccc tggtagcaat gctactggga
     7021 atggatgttt atggctggca agtgatggcg ttaacgctac tgctgggaac ccctacgctt
     7081 ggctttcttg gtgcgccggg cgtggcgctg acagtgggac ttaagcgcgg tggtgtgctg
     7141 ctcagcatac tggtgttacc gctgactatc ccattactca tctttgccac cgccgcgatg
     7201 gacgcggctt ctatgcattt gcccgttgac gggtatctgg caattttagg cgcgttgctg
     7261 gcaggcaccg cgacattaag tccttttgcg acggcggcag cgttacgaat cagcattcaa
     7321 taacgctgga ttactgattt ttgttcgagt ctggtatcga aactatgtgg aaaacactgc
     7381 atcaactggc gatcccacca cggctgtatc aaatctgtgg ctggtttata ccgtggctgg
     7441 caattgccag tgtggtcgtg cttaccgtcg gctggatctg gggattcggc tttgctccgg
     7501 ctgattatca gcagggaaat agctaccgca ttatctacct gcatgtgcct gcggcgatct
     7561 ggtcgatggg catttatgca tcaatggcgg tggcggcttt tattggcctt gtctggcaga
     7621 tgaaaatggc caacctggcg gtggcggcga tggcccccat tggtgccgtg tttaccttta
     7681 ttgccctggt taccggctct gcatggggga aaccgatgtg gggcacctgg tgggtatggg
     7741 atgcacgtct gacttctgaa ctggtgctgc tgtttttgta tgtgggtgtg attgccctgt
     7801 ggcacgcctt cgacgaccgc cgtctggcgg gccgtgcggc aggtatcctg gtgctgattg
     7861 gcgtggtgaa tctgccgatt attcattact ccgtggagtg gtggaacacc ctgcatcagg
     7921 gatcaacgcg gatgcagcaa agtatcgatc ctgcgatgcg ttcgccgctg cgctggtcga
     7981 tttttggctt cctgctgctg tctgccacgc tgacgctgat gcggatgcgt aatttgattt
     8041 tgctgatgga aaaacgccgt ccgtgggtga gtgaactgat actgaaaaga ggccgtaaat
     8101 gacccctgca tttgcttcct ggaatgaatt tttcgcaatg ggcggttacg ccttttttgt
     8161 ctggctggcg gtggtgatga ccgttattcc gctggtggtt ttggtcgtgc actcggtgat
     8221 gcagcatcgc gcaattctgc gtagcgtggc gcaacagcgg gcgcgagagg cgcgtttacg
     8281 tgctgcacaa cagcaggagg ctgcatgaat attcgccgta aaaaccgctt gtggattgcc
     8341 tgtgccgtgt tggcagggct ggcgctgact atcggtctgg tgctatatgc gctgcgctcg
     8401 aatatcgatc tcttttatac gccgggggaa attctctacg gcaagcgtga aactcagcag
     8461 atgccggaag tcggtcagcg tctgcgcgtt ggcgggatgg tgatgccggg tagcgtgcag
     8521 cgcgatccca attcgctgaa agtaacgttc accatttacg atgctgaagg ctcagtggat
     8581 gtctcttacg aaggcatttt gccggatctg ttccgtgaag ggcagggcgt tgtggtgcag
     8641 ggcgagctgg aaaaaggcaa tcatatcctc gcgaaagaag tgctggcgaa acacgacgaa
     8701 aactacacgc cgccagaagt tgagaaagcg atggaagcta accaccgtcg cccggcgagt
     8761 gtttataagg acccagcatc atgatgccgg aaattggtaa cgggctgctg tgcctggcgc
     8821 taggaattgc gctgctgctg tctgtgtatc cgctatgggg cgtggcgcgc ggagatgcgc
     8881 gcatgatggc gtcttcccgc ttgtttgcct ggctgctgtt tatgtctgta gctggcgcat
     8941 ttctggtgct ggtcaatgct ttcgtggtca acgacttcac cgtcacctat gttgccagca
     9001 actccaatac ccagcttccg gtgtggtatc gcgtggcggc tacctggggt gcacatgaag
     9061 gctcactgct gctgtgggtg ctgctgatga gcggctggac ctttgcggtg gcgattttta
     9121 gtcagcgtat tccgctggat attgtggccc gcgtactggc gataatgggg atggtcagcg
     9181 tcggcttttt gctgttcatt ctctttacct ctaacccatt ctcacgcacg ctgccgaact
     9241 tcccgattga agggcgcgat cttaacccgc tgttgcagga tccggggctg atcttccatc
     9301 cgcctctgct ctatatgggg tacgtgggtt tctcggtggc gtttgctttt gccattgctt
     9361 ctttgttgag cgggcgtctg gacagcactt atgcgcgttt tactcgtccg tggacgctgg
     9421 cagcgtggat cttcctgacg ctcggcatcg tgctcggttc cgcatgggcc tattacgaac
     9481 tcggctgggg tggctggtgg ttctgggatc cggtagaaaa cgcctcgttt atgccgtggc
     9541 tggtggggac tgcgttgatg cactcgctgg cggtgactga acagcgtgcc agcttcaaag
     9601 cgtggacatt actgctggca atcagtgcct tctcgttgtg tctgctgggg actttcctcg
     9661 tgcgttccgg cgtgctggta tcggtacacg cgtttgcgtc tgatccggcg cgcggtatgt
     9721 ttatcctcgc ctttatggtg ctggtgattg gcggttcgct gctgctgttt gccgcgcgtg
     9781 gacacaaagt tcgctcacgc gtaaacaatg cgctgtggtc gcgggaatct ttgctgttag
     9841 cgaacaacgt tttgctggtc gccgcgatgc tggtggtgtt gctggggacg ctgctgccgc
     9901 tggtgcacaa acaactgggg ctgggcagta tttcgattgg cgaaccgttc ttcaacacca
     9961 tgtttacctg gctgatggtg ccgtttgcgc tactgcttgg tgtcggtcct ctggtgcgct
    10021 gggggcgcga tcgcccacgt aaaatccgca atttattgat tatcgccttc atctctacgc
    10081 tggtgctgtc tctgctgttg ccgtggctgt tcgaaagcaa agttgtggcg atgacggtgc
    10141 tcggcctggc aatggcctgc tggattgcgg tgctggcaat tgcagaagcg gcgctgcgta
    10201 tttcacgcgg cacgaaaacc accttcagct attgggggat ggtggcggct cacctggggc
    10261 tggcagtgac aattgttggt attgctttca gtcagaacta cagcgttgag cgtgatgtgc
    10321 gcatgaagtc cggcgatagc gtcgatattc atgaatatcg cttcaccttc cgtgatgtta
    10381 aagaggtgac tggtccgaac tggcgcggcg gtgtggcgac tatcggcgta acgcgagatg
    10441 gcaagccgga aacggtgctg tatgcggaaa aacgttatta caacactgcc gggtcgatga
    10501 tgaccgaagc ggcgattgac ggcggcatca cccgtgacct gtacgcggca ctcggtgaag
    10561 aactggaaaa cggtgcctgg gctgttcgtc tttactacaa accgtttgtt cgctgga
//
LOCUS       DAZFKF010000143        10394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFKF010000143 DAZFKF010000000
VERSION     DAZFKF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.145"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(325..1725)
                     /gene="asnS"
                     /locus_tag="ACHKA1_004657"
     CDS             complement(325..1725)
                     /gene="asnS"
                     /locus_tag="ACHKA1_004657"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO5682212.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1893..3095)
                     /gene="pncB"
                     /locus_tag="ACHKA1_004658"
     CDS             complement(1893..3095)
                     /gene="pncB"
                     /locus_tag="ACHKA1_004658"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO5682213.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLRNFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3361..5973
                     /gene="pepN"
                     /locus_tag="ACHKA1_004659"
     CDS             3361..5973
                     /gene="pepN"
                     /locus_tag="ACHKA1_004659"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO5682214.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(6016..6783)
                     /gene="ssuB"
                     /locus_tag="ACHKA1_004660"
     CDS             complement(6016..6783)
                     /gene="ssuB"
                     /locus_tag="ACHKA1_004660"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO5682215.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(6780..7571)
                     /gene="ssuC"
                     /locus_tag="ACHKA1_004661"
     CDS             complement(6780..7571)
                     /gene="ssuC"
                     /locus_tag="ACHKA1_004661"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO5682216.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(7583..8728)
                     /gene="ssuD"
                     /locus_tag="ACHKA1_004662"
     CDS             complement(7583..8728)
                     /gene="ssuD"
                     /locus_tag="ACHKA1_004662"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO5682217.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQDLAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(8725..9684)
                     /gene="ssuA"
                     /locus_tag="ACHKA1_004663"
     CDS             complement(8725..9684)
                     /gene="ssuA"
                     /locus_tag="ACHKA1_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO5682218.1"
                     /translation="MRKIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLATRPY
                     AVKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(9677..10252)
                     /gene="ssuE"
                     /locus_tag="ACHKA1_004664"
     CDS             complement(9677..10252)
                     /gene="ssuE"
                     /locus_tag="ACHKA1_004664"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO5682219.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALHTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
BASE COUNT         2600 a         2800 c         2652 g         2342 t
ORIGIN      
        1 tctatttata tgatttcctt atatttaaat taactaaacg gaaattttgt ttctgatgga
       61 aactttatca acctggcaca aaatcctctt tcagacatcc agaacgccaa aaatagtatg
      121 aaaattgtgc tatcagctaa gaaaaaacta atccgcaata agaatgcgga ttattttttg
      181 cgcataaatg ttgcaatgta atattccgta acaggatgat cgttatggcg gggttgcaaa
      241 tagattgctt gacttaacaa acagataact tgacagaaaa gataaaaaaa gagccagcgg
      301 gcgctggctc ttgaagacgc taaattagaa gctggcgtta cgcggagtac gtgggaacgg
      361 aatcacatca cgcacgtttt gcacaccagt tacgtaagca atcaggcgtt caaaaccaag
      421 accgaaacct gaatgcggaa cagtaccgta gcgacgcaga tcgcgatacc accagtaatc
      481 ttctttattc aggcccattt ccagcatacg ctcgtccagc acgtccagac gttcttcacg
      541 ctgggagcca ccaatgatct caccgatgcc cggagccaga acgtccatag ccgcaacggt
      601 tttaccgtct tcgttaagac gcatatagaa cgctttaata tctttcggat agtttttaac
      661 cactaccggt gctttaaagt gttcttccgc cagataacgt tcatgctcag aagagagatc
      721 aacgccccag taaaccgggt tttcaaactt cctgccgcag ttttcgagaa tggtcactgc
      781 gtcggtgtaa tccacctgcg caaaatcggc ttcaatgaag cgttccagac gtgaaacggc
      841 atctttatct acgcgttcag cgaagaattt catgtcgtca gcgcgttctt cgagaaccgc
      901 tttgaagaca tatttcagca tggcttcagc cagtcccgcg atatcgttca ggttagcaaa
      961 cgccacttcc ggttccagca tccagaattc cgccaggtga cggctggtgt tggagttttc
     1021 agcacggaaa gtcgggccga aggtataaat tttggacaat gcgcaagcgt aggtttcgcc
     1081 gttcaactgg ccagatacgg tcaggaaaga ctctttacca aagaagtctt tgtcgaaatc
     1141 cactttgccc tgatcgttac gcggcaggtt ttccagatcc agcgtagaaa cgcggaacat
     1201 ttcgccagca ccttcggtat cagatgcggt aatcagtggc gttgaaaccc agaagaatcc
     1261 ctgctcgtta aagaagcgat gcagcgcctg cgccagcgta tgacgaacgc gcgcgacggc
     1321 accaatcagg tttgtgcgcg gacgcaggtg agcgacttca cgcagatact caatgctgtg
     1381 gcgttttgcc gccatcgggt acgtgtctgg atcttcaacc caaccagcaa cttcaacctt
     1441 gctggcctga atttcaaatt gttgcccctg gcccggcgac gccacgactt tacccgtcac
     1501 aatgaccgag caaccggtgg tcagacgcag gacgtcttca ttgtaattgg gcagagaatt
     1561 attgatgaca gcctgtacag gatcaaagca ggaaccgtca taaacggcga ggaaggagat
     1621 gccagctttt gaatctcggc gggtacgtac ccatccgcgt acggtgactt cgctgtcaac
     1681 ggctacgcgg ccctggagta cgtcggctac aggcacaacg ctcataatat tctctctgtt
     1741 aatagtcgga aaaaataaac acttgtccac ccgtaaatgg ggggatacct atgttacctg
     1801 gcatctgcaa tcagacaagc agaattcgca aatgcagcga aagatttcgg aattaaaggt
     1861 aaaaaaggga gccgattagc tccctgatga tattaactgg cttttttaat atgcggaagg
     1921 tcgaacgctt tgcgcagcgc ccgaacaaac gctttatcgt ggcagatagt tttgccaggg
     1981 ctgtcagaaa gtttcgccac cggtttaccg ttacactcta ccaacttaat cacaatattc
     2041 aggggtttta cctgggggat atcgcaggtc aggcgagtcc caataccaaa acttaattgc
     2101 acgcgggaag agaagtggcg gtatagctca accgctttgc gtaaatccag attgtcagag
     2161 aaaaccagcg ttttactctg tggatcaatt cccagctttt cataatgtgc aatggctttt
     2221 tcaccccatt caaccgggtc gccagagtca tgacgcaggc cctgataccg actagcgaac
     2281 tcgacaccga aatcacgcag gaaagcatcc atagtgatgc agtcggttaa tgcaatgcca
     2341 agttggtcgg gatactcttc cagccaggca gcaagtgcag ctcgctggct gttggctaga
     2401 tccgggctga tttgctgatg tgcctggaac cattcgtgtg cctgtgttcc catcggcgtg
     2461 agggaaagcc gacgcgccag atcgtagttg ctggtgccca cgaaccaaga ttcctgttgc
     2521 agacgcttaa cgatggtttc ttgtacttcg cgagaaaaac ggcgacgggt gccaaaatcc
     2581 atcagatgga agcgcgacat atcaagaccg gcggttaacg ccgagaagtc gactaatttg
     2641 ctttccagcg tgtcgagggc ttgcgcaacg tcggcctgcg gtgagcgata gcgatgtacc
     2701 atttcactga taaccgccag caaaggaact tcccagagga tgacttcacg ccacgggccg
     2761 cttaaacgaa tatccagctt gccattatcg ttggacacgg tgacttgttc cgggttaaag
     2821 cggaaattac gcagccagtt gagatagtcg gccttaaaga aaggcagcgc agagagccat
     2881 tgatattcat catcctgcag gcgcaggtgc tgcatcgcct gaacctgttc acgaatagca
     2941 tcggcataaa tacccagcag atcgtcacct cggcaacgaa actccgccgc gacatgcaca
     3001 tcgtaatagt gatgaaacac ggcttgctgc atatgcaact tataagcatc tgtatccagc
     3061 aacgagtgca gaacaggaga agcgaattgt gtcataggtg cgcagtagcg tcctcgtaca
     3121 ggagcgttta gtacaataaa catcttcagg aactgctgga gtataccttg tttcgcaatt
     3181 tattgaaccc cgatcacacc atatgccacc tttctggtcg atggcattac gcggtgtatg
     3241 ttataaaaat gtagcaataa aggcgtttgt acctgaaaag atgaagattc tgcatagcgc
     3301 gatttacgca acaggaatag actgaacacc agactctata aaagatgcta aaggttattt
     3361 atgactcaac agccacaagc caaataccgt cacgattatc gtgcgccgga ttaccagatt
     3421 actgatattg acttgacctt tgacctcgac gcgcaaaaga cggtcgttac cgcggtcagc
     3481 caggctgtcc gtcatggtgc atcagatgct ccccttcgtc tcaacggcga agacctcaaa
     3541 ctggtttctg ttcatattaa tgatgagccg tggaccgcct ggaaagaaga agagggcgca
     3601 ctggtcatca gtaatttgcc ggagcgtttt acgcttaaga tcattaatga aataagcccg
     3661 gcggcgaata cggcgctgga agggctttat cagtcaggcg atgcgctttg cacccagtgt
     3721 gaagccgaag gtttccgcca tattacgtat tatctcgacc gcccggacgt gctggcgcgt
     3781 tttaccacca aaattattgc cgataaaatc aaatatccct tcctgctttc caatggtaac
     3841 cgcgttgcgc aaggcgaact ggaaaacgga cgccattggg tacagtggca ggacccgttc
     3901 ccgaaaccgt gctacctgtt tgcgctggtg gcaggcgact ttgatgtact gcgcgatacc
     3961 tttaccacgc gttctggtcg cgaagtagca ctggagctgt acgtcgatcg cggcaacctt
     4021 gatcgcgcgc cgtgggcgat gacctcgctg aaaaactcca tgaaatggga tgaagaacgc
     4081 tttggcctgg agtatgacct cgacatctat atgatcgtcg cggtggattt cttcaatatg
     4141 ggcgcaatgg agaataaggg gctgaatatc tttaactcca aatatgtgct ggcccgcacc
     4201 gataccgcca ccgacaaaga ttacctcgat attgaacgcg ttatcggcca tgaatatttc
     4261 cataactgga ccggtaaccg agtgacctgc cgcgactggt tccagctcag cctgaaagaa
     4321 ggtttaaccg tcttccgcga tcaggaattc agctctgacc tcggttcccg cgcggtaaac
     4381 cgtatcaata atgtgcgcac catgcgcgga ttgcagtttg cggaagacgc cagcccgatg
     4441 gcgcacccaa tccgcccgga tatggtcatt gagatgaaca acttctacac cctgaccgtt
     4501 tacgagaagg gcgcggaggt gattcgcatg atccacaccc tgcttggcga agaaaatttc
     4561 cagaaaggga tgcagctcta tttcgagcgt catgatggta gcgcagcgac ctgtgacgat
     4621 tttgtgcagg cgatggaaga tgcgtcgaat gtcgatctct cccatttccg ccgttggtac
     4681 agccagtccg gtacgccggt tgtgaccgtc aaagacgact acaatccgga aaccgagcag
     4741 tacaccctga ccatcagcca gcgcacgcca gccacgccgg atcaggcaga aaaacagccg
     4801 ctgcatattc cgtttgccat cgaactgtat gataacgaag gcaaagtgat cccgttgcag
     4861 aaaggcggtc atccggtgaa ttccgtgctg aacgtcaccc aggcggaaca gacctttgtt
     4921 tttgataatg tctacttcca gccggtgcct gcgctgctgt gcgaattctc tgcgccagtg
     4981 aaactggaat ataagtggag cgatcagcaa ctgaccttcc tgatgcgtca tgcgcgtaat
     5041 gatttctccc gttgggatgc ggcgcaaagt ttgctggcaa cctacatcaa gctgaacgtc
     5101 gcccgtcatc agcaagggca gccgctgtct ctgccggtgc acgttgctga tgctttccgc
     5161 gcagtgttgc tcgatgaaaa aatcgatccg gcgctggcgg cagaaattct gacgctgcct
     5221 tcggtaaatg aaatggcgga actgttcgat atcatcgacc cgattgccat tgccgaagtc
     5281 cgtgaagcac tcactcgtac tctggcgaca gaactggcag atgagctgct ggctatttac
     5341 aacgcgaatt accagagcga gtaccgcgtt gagcatgaag atattgcgaa acgcactctg
     5401 cgtaatgcct gcctgcgttt cctcgctttt ggtgaaacgc atctggctga tgtactggtg
     5461 agcaagcagt accacgaagc gaacaatatg actgatgcgt tggcggcgct ttctgcggcg
     5521 gttgctgcac agctgccatg ccgtgacgcg ctgatgcagg agtacgacga caagtggcat
     5581 caggacggtc tggtgatgga taaatggttt atcctgcaag ccaccagccc ggcggcgaat
     5641 gtgctggaga cggtgcgcgg cctgttgcag catcgctcat ttaccatgag caacccgaac
     5701 cgtattcgtt cgttgattgg tgcgtttgcg ggcagcaacc cggcagcgtt ccatgccgaa
     5761 gatgggagcg gttaccagtt cctggtggaa atgcttaccg acctcaacag ccgtaacccg
     5821 caggtggctt cgcgcctgat tgagccgctg attcgcctga aacgttatga tgccaaacgt
     5881 caggagaaaa tgcgcgcggc gctggaacag ttgaaagggc tggaaaatct ctctggcgat
     5941 ctgtacgaga agatcaccaa agcactggct taataaataa ccgaatggcg gcaatagcgc
     6001 cgccattcgg ggattttacc cctgttttct caggcgagtt tcagactcac cacgctgcat
     6061 tacccgttgc aacacttccg cttccagctc cgctaatcgc accgacccta accggcgcgg
     6121 acgggggata tccaccgtca aatccagacc aatttttccc tcttcaatta acaacacccg
     6181 gtcagccatt gctaccgctt cgctcacatc atgcgtcacc aacagtacgg taaagccgtg
     6241 ctcctgccaa agtgacacaa tcaaatcctg catctcgagt cgcgttaagg catccagcgc
     6301 cccgagtggt tcatcaagca acaataaacc cggtcgatga atcaacgctc ttgccagcgc
     6361 cactcgctgt ttctgcccgc cagaaagtgc ggcaggccat tccccggcgc gattctccag
     6421 tcctaccgca gccaacgccc gacgcgcggc atcgcgccac tggcctttaa gacctaaccc
     6481 aacgttatca atcaccgatt tccacggcag cagacgcgca tcctgaaaca tcattcgcgt
     6541 gtcttcctga atttcagcca gcggtgtggt gcccgctaac acatcgccag cggttggcgt
     6601 ttccagacct gccagcaggc gcagcagggt acttttgcca ccaccgctgc ggcccaccac
     6661 cgccacaaac tgacctgccg gaatatgtaa atccagttgg ttcaggacga tattttctgc
     6721 gtaatgtttg cttactgcat tgagcaacaa tggtgtgccc tggttcagac gagcagtatt
     6781 cataccgtgg cctccttcaa atgataagcc gggttccagc gcaaccagag gcgctctaac
     6841 agctgcgcgc tgacgtcagc cagtttgccg agcagggcgt aaagaataat ggcgaccacc
     6901 accacgtccg tttgcaaaaa ctcccgcgca ttcatcgcca gataaccaat gcctgaattg
     6961 gcagaaatgg tttcggcaac aatcagcgtc agccacatca ggcccaacgc aaaacgcacg
     7021 ccgaccataa ttgagggcag ggcaccaggc aggatcacat ggataaacag cggtatgccg
     7081 gataatccat agctacgcgc catctccacc aacccgcgat cgatattacg gatgccatgc
     7141 caggtgttga tataaatggg gaatagcgta ccgagcgcca ccagaaagat ttttgcggat
     7201 tcatcaatgc caaaccacaa aatcaccagc gggatcagcg ccagatgcgg cacgttgcgc
     7261 aacatctgaa ttgaggtatc cagcagccgt tctccccagc gagatagccc gctaatcagt
     7321 cccaaaatca accccagcga tccgccaatt gaaaagccaa tcagcgcccg ccaggagctg
     7381 atcgccagat gctgccacag ttcgccgctg gcggagagcg tccagaacgc cgtcaccact
     7441 ccttccggtg aaggcaaaat acgcgtcgac agccagccaa ccgaggaggc cagttgccac
     7501 accgccacga tgcccaccgg taaaaaccag ggggcaacgc gcaataacca cttcttcact
     7561 ggcgttgcca tcatcgtgtt ccttagcttt gcgcgacttt acgggggata aaatcattcg
     7621 ccaccgcttc gccttgcgga ttcagcggct gcggctgggg aatttccgga atggcgacat
     7681 ccagatgtgg gaacagcaac tcgccgaccc gatacgcttc ttccagatgc ggatagcccg
     7741 aaagcacaaa gctgtcgatg ccaagcgcgg catattcgtt gattcgcgca gcgaccgtag
     7801 gaccatcgcc caccagcgcc gtcccggcac cgccgcgcac taagccaacg cccgcccata
     7861 aattggggct gatctccaga ttgtcgcgct tgccattatg caacgccgcc attcgctgtt
     7921 gccccacgga atccgtgcgg gcaaacgcgg cctgtgcttt ggcgatagtc tcatcatcaa
     7981 gatgcgaaat taaccgttcg gcggcctgcc acgcttcgtc gttggtttcg cgaacaatca
     8041 catgcagacg aataccgaaa cgaattttgc gtccatgcgc ggccgctttc gcccgcactt
     8101 gttcgatttt ctcttttacc agctctggcg gttcgcccca ggtgagataa agatcaactt
     8161 gttcggcggc cagatcctga gcgacgtctg aagatccgcc aaagtaaagc gggggataag
     8221 gctgttgaat cggcggaaag agcagtttgg ctccacgcac atgaatatgt ttgccgttga
     8281 aatcgacggt ttcgcccagc aataaacgcc gccagacctg ggtaaattcc gccgaggctt
     8341 cgtagcgctc gctatgatca aggaacacgc catcgcctgc cagctcttgt ggatcgctgc
     8401 ctgtgaccag gttaaataac gcacgtccgt tagagagacg gtcaagcgtg gcggcctggc
     8461 gggcggcaac ggtaggagac gtgacactgg gacgcagagc gacaagaaac ttcagccgct
     8521 gcgtcaccgg gatcatcgac gctgcaacca gccacgcatc ttcgcaggag cgccccgttg
     8581 ggatcagcac accggtatag cccagacgat ccgccgcttg cgcaatttgt tgcagataac
     8641 cgtgatcaac cgggcgtgaa ccttcttccg ttcccagata atgcccgtca ccgtgggtcg
     8701 gtaaaaacca gaacatattc agactcataa ttgttttcct tccagttgag tgggctgcca
     8761 gatgcgctgg cgaatatcga ctttttttgg caccagacgg ttttcataaa acagatctgc
     8821 cgtttgctgt tgtaaggcag caacttctgc gctcaccggc ttgatagtgg tagggggacg
     8881 atgatctaaa taagaggcaa tcaccggcgc cggtaagccc atcgtttttg ccagtaaagc
     8941 gatactttgc tcgcgctgac tgcgggttaa cgcatcggct tcgctaaagg ttgccagtac
     9001 gccctgaata aatgcgccgt tttttactgc atacggacgg gttgccagat aaaacgatcc
     9061 ggtttgattg aggtcggtac cgtctttcag cacccgcacg ccgccctgta ataacgcggc
     9121 ggagtagtag ggatcccaga tagcccaggc atcgacgtta ccctgctgga atgcggcgcg
     9181 ggcatcagct ggcgtcagat aagtgggctg gatgtcagta aacttaagtc cggcctggcg
     9241 tagtgcgcgc agtaaaagat tgtgtgaact ggaacctttc tggaacgcaa ctttgtggcc
     9301 tttaagatcg gctacggttt tgatcgggct gttttctgcc accagaatca cctcggcttt
     9361 tggcttcggc ggctcgacgc ccacatatac caaatcagcc ccggcagcct gggcaaagat
     9421 tggcggaata tccccggtac tgccgagatc aatactgcca acgtttaacg cttctaacat
     9481 ttgcggaccc gcggggaact ccacccagga gatttttgat tgcggatagc gtttttccag
     9541 taactggtgg ctttttgcca ataccatacc aatactgcct ttctgatagc ctatacgtaa
     9601 cgcttcaggc gaggattctg cagcaaccac aatcgtagag acgctaagca atcccaccag
     9661 cgccagttta atgatcttac gcatgggcat tacctcgcag agaatgaaga tcaggaacct
     9721 gaacatcgcg gcggtgcaat gcctgccaga aagtttctag cgcggtgtca agacgggttt
     9781 gtaaattcgg cgtgaactgg ggtttgtgat ggtaatcaat tacttgtgag tcatcggcaa
     9841 acacgccgtg caggatctcc tgggctttca gcgcacttaa aactggctta agggcataat
     9901 cgaccgccag cagatgggcc accgtaccgc ccgtcgccag cggtagcacc actttgcctt
     9961 gcaacgcacg ttctggcagc aggtcgagca gggttttcag cgcaccggaa taagcggctt
    10021 tatacacagg cgtggcgaca atcagcccgt cagcctgttg caactgttcg gtgaaggtat
    10081 ggagtgccgg actatcgaaa cgagcataaa gcagatcttc cgcggcgaag ttttgcagat
    10141 tccagtgata aacctcaaca tccaggccat ttagtttttc ccgcgcatat tccagcaagg
    10201 agctggagcg agaaggaaag cgaggactac ccgccagggt gatgacacgc atactctctc
    10261 cttataacca attgttcttg ttttgttaac attgataaca atttgctcag tctgtcggag
    10321 agacaagaaa atgccaaata taaattttgt gtatcttttt ctgaaaataa actaaagaaa
    10381 gggctttata tatc
//
LOCUS       DAZFKF010000144        10056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFKF010000144 DAZFKF010000000
VERSION     DAZFKF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.146"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..147)
                     /locus_tag="ACHKA1_004665"
     CDS             complement(<1..147)
                     /locus_tag="ACHKA1_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008807599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO5682220.1"
                     /translation="MEFHKNKAKTPFIRLVLLWHAMRRWRRQMQTRRVLQQMSDERLR
                     DIGLR"
     gene            324..1736
                     /gene="yjiR"
                     /locus_tag="ACHKA1_004666"
     CDS             324..1736
                     /gene="yjiR"
                     /locus_tag="ACHKA1_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO5682221.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            complement(1978..2922)
                     /locus_tag="ACHKA1_004667"
     CDS             complement(1978..2922)
                     /locus_tag="ACHKA1_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO5682222.1"
                     /translation="MTNFTTSTPHDALFKSFLMHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRQLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVILLVTECANDSQITALLNYIL
                     LTGDEARFNEFISELTRRMPQQRERIMTIAERIHNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQRYTGLSAEQMQALEQPLPESKRDPWIEY"
     gene            3389..4621
                     /gene="mdtM"
                     /locus_tag="ACHKA1_004668"
     CDS             3389..4621
                     /gene="mdtM"
                     /locus_tag="ACHKA1_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO5682223.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSMLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            4662..5942
                     /gene="yjiN"
                     /locus_tag="ACHKA1_004669"
     CDS             4662..5942
                     /gene="yjiN"
                     /locus_tag="ACHKA1_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO5682224.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            6058..7209
                     /gene="yjiM"
                     /locus_tag="ACHKA1_004670"
     CDS             6058..7209
                     /gene="yjiM"
                     /locus_tag="ACHKA1_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO5682225.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQREEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            7219..7986
                     /gene="yjiL"
                     /locus_tag="ACHKA1_004671"
     CDS             7219..7986
                     /gene="yjiL"
                     /locus_tag="ACHKA1_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO5682226.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDCITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            7983..8249
                     /locus_tag="ACHKA1_004672"
     CDS             7983..8249
                     /locus_tag="ACHKA1_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO5682227.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
     gene            8562..>10056
                     /locus_tag="ACHKA1_004673"
     CDS             8562..>10056
                     /locus_tag="ACHKA1_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO5682228.1"
                     /translation="MGSLNIYQDIFPTLGMYSGLKSCHEKNNQPFDINTEIETIQKQI
                     NYDINHLNDGLIKRVLNLFIHLISNPDNLELTLNRYSSTTEQIIGRTKRNGLHEFDVG
                     DLKIIFNRQDDNESVLTVKYKDKDKDISHHCNVKTEQLQQFIKIMEQKAQLSIYIDKN
                     NLKESIFSVLHNDPQHVDKDQHLPCEKFLKHACKSSNSFEVKLDATHQYQHLNNFMIS
                     FDPVENQLTIRDNNNKTETFSFTNLQWENLLQYYKENHQQPNIAGSRNLTDNIDKIKN
                     TISTSEIIECASPEIGSSVLNDLYSIANFLPDNNLTPNESWKRFCDTCERFYVAQKSI
                     TGDKSERLTRKLSISDAGITMTFKIGDVVINTIITAIPEDATGQRCIEGLNLAEMDLT
                     GIDLSKMVLRNVNFNGSILRHAKFSGTICEGVDFTDCDLRNAKFKNASLENNDFRKVR
                     HLTYVNFKNANLRNSNFSGKVLTGVNFTGSNLSNAYLEYIDFTTVILY"
BASE COUNT         2471 a         2420 c         2509 g         2656 t
ORIGIN      
        1 gcgtaaccca atatccctca accgctcatc actcatctgc tgtaacactc gtcgggtctg
       61 catttgccgc cgccaacgcc tcatcgcatg ccagagtagc acaagtctga taaacggcgt
      121 tttggctttg tttttgtgaa attccatctg ctgcctcctc atctttcaga ggcttcatca
      181 tccgccttct ggcagttcac aatacagatg caaatattag ttttatttaa catacagaca
      241 ggttaaaaca gcatctgcat ccggtttttt agcctcatct gtaccggttt ttctctctgt
      301 atggtctaaa taaggaatac agcatgacgc gttatcaaca tctggcgact ctacttgccg
      361 agcggattga gcaggggctg tatcgtcacg gggagaaatt gccgtcggtg cgcagcttaa
      421 gtcaggagca cggcgtcagc atcagtaccg tgcagcaggc gtatcagacg ctggagacga
      481 tgaagctcat cactccgcag ccgcgttcgg ggtattttgt cgcacaacgt aaagcccagc
      541 cgccagtgcc gccgatgacg cgtccggtgc agcgcccggt ggaaattacc cagtgggatc
      601 aggtgctgga tatgctggag gcgcatagcg acagttccat tgttccgtta agcaaaagca
      661 cgccggatgt cgaaacgccc agcctgaaac cgttgtggcg tgagctaagc cgggtggtgc
      721 agcataatct gcaaaccgtt ctcggttatg acttgttagc cggtcagcgg gtattgcgag
      781 agcagattgc ccgcctgatg ctcgacagcg gctcggtggt caccgccgat gacatcatca
      841 tcaccagcgg ctgccataac tcgatgtcgc tggcgttaat ggcggtgtgt aaaccgggcg
      901 atattgtcgc ggtcgaatct ccctgttatt acggttcgat gcagatgctg cgcggcatgg
      961 gcgtgaaagt gattgaaatc ccaaccgatc cagaaactgg catcagcgtt gaagcgctgg
     1021 aactggcgct ggaacagtgg ccgattaaag gcatcattct ggtgccaaac tgtaataatc
     1081 cgctgggatt tattatgccg gacgcgcgca aacgggccgt tctctctctc gctcagcgtc
     1141 atgatattgt gatttttgaa gatgatgtct acggcgaact ggcaacggag tatccgcgcc
     1201 cgcggaccat tcattcatgg gatatcgacg ggcgagtgct gttgtgcagc tcgttcagta
     1261 aaagtattgc acctggcctg cgcgtgggct gggtcgcacc ggggcgttat cacgataaac
     1321 tgctgcatat gaaatacgcc atcagcagct ttaatgtacc gtctacgcaa atggcggcgg
     1381 cgacgtttgt gctggaaggt cactatcatc gccatatccg gcggatgcgg cagatctatc
     1441 agcgcaattt ggcgctttat acctgctgga tacgggaata ttttccctgc gaaatctgta
     1501 ttacgcgccc gaaaggcgga tttttactgt ggatcgaatt gcctgaacag gtcgatatgg
     1561 tctgcgtcgc gcggcagcta taccgcatga aaatccaggt ggcggcaggc tcgattttct
     1621 cggcttccgg caaataccgt aattgtctac gtatcaactg cgctttgccg ctcagcgaaa
     1681 cctatcgcga agcactgaag caaattggcg aggccgtgta tcaggcaatg gaatgaattc
     1741 gaattagtgg cgaggcccct gatttcctct cacgaatcgc ctgtgactcg ttttatcttt
     1801 gcaggggcat cgccacgttc cagcatggct taggcaaagt gtgctttgtt cataccggat
     1861 gcggcgtgaa cgccttatcc ggcttacaaa tcgcgcaaat tcaatagatt gcagaggtaa
     1921 tgtaggtctg ataagcgtag cacatcaggc agctttgcgt ttgtcatcag tctctgatta
     1981 gtactcgatc catggatcgc gcttgctttc aggcaagggt tgctctaatg cctgcatttg
     2041 ctctgccgaa agtccagtat atcgttgtat ccattcagga tccgcaccgt tctgcaaaag
     2101 caatctaagg aggcgttgtt ccccttcttc tttccccttt tccatcccta acagccatcc
     2161 atcattatga attcgctctg caatcgtcat tattcgctcc ctttgttgtg gcattcgacg
     2221 ggtaagttca ctgataaact cattaaaacg cgcttcatcg ccagtcagta aaatgtaatt
     2281 taacagcgca gttatctggc tgtcattagc acactctgta accagtaata ttaccagttg
     2341 atcgataagc cccatcagat cgcgctggcg aatatgcttt tggatcaact ccaacagggc
     2401 gactctgcgg tgctgcacaa tctcgtcgtc tggcacgaca gtgacatcca ccagcgggaa
     2461 cgctgcagta taaagtttcc gtgcagtagt cgggtcggca aattcgtcca gccagcacag
     2521 ggaccagggg taaggactac ggctaccgtg ataaaacagc atcgggatga ccagcggtag
     2581 ctgccggcgt ttatcatgct cgatatggcg ctgcatcacc gccatggaat atcgcatcag
     2641 gcgaaaggcc atatggatat cctcgcggct ctgatgttca atcactacgt aaatataacc
     2701 atcaccttca cgggtcttta ccgaccacag aatatcggag tgtagcgccc gcaatttttc
     2761 atcgacaaag ctggcggatt ccagttttaa gctgtcgaga tcgcacagtt cacgtaaatc
     2821 tttgggtaag tggatctcca taaaatcccg cgcggtgtca gggtgcatga gaaaggactt
     2881 aaataatgcg tcatgtggcg tgctggtcgt gaagtttgtc atggcgatcc gtcacctgta
     2941 gataagatgg cccgacttta gcgactaaca aaaccattct cacccggcaa tttctcgttc
     3001 cccgaggtgc tttccaggct atttttgttc gtctgcaaac gatacatttt tccggaaaca
     3061 ggctcgcgtt ttacatgtcc tgtacccggc agatattccc ctttccaccg cgctcttttg
     3121 attaaccgca aattttaatt acacttaagg tgtatatttt ctatgcaacc catcaattca
     3181 agaggtgtaa tgtgctgatg actatttgta atcgttatac attctgaccc gaagtcagaa
     3241 ggtatttctc tgtctgtgtg ttcacaggca gtgtgattga ttacatgaaa ttcagtacat
     3301 ttgtccttct cgcctgcctt tcgtcattac cgacggtatt gaatttcgtt ttccccattg
     3361 gggttctccg gacaaggagt tgtttgttat gccacgtttt tttgcccgcc atgccgctac
     3421 gctgtttttc ccgatggcgt tgattttgta tgactttgcc gcctatcttt cgacggatct
     3481 gatccagccg gggatcatta atgtggtccg tgattttaat gccgatgtca gtctggcccc
     3541 tgctgccgtc agtctctatc tcgctggcgg tatggcgtta cagtggctgc tggggccact
     3601 ttccgacagg attggccgca ggccagtgct gattaccggg gcgctcattt ttacccttgc
     3661 ctgcgctgcg acaatgttca caacgtctat gacacagttt cttatcgcgc gtgcaattca
     3721 gggcaccagt atctgtttta ttgctaccgt tggttatgtc acggtgcagg aggcgttcgg
     3781 acagacaaaa gggatcaagt tgatggcgat tatcacctcc atcgtactga ttgcgccgat
     3841 tattggcccg ctttccggag cagctctgat gcactttgtg cactggaaag tcctttttgc
     3901 catcattgcg gttatgggtt ttatctcatt tgttggctta ctgctggcga tgccagagac
     3961 ggtgaagcgc ggcgcggttc catttagcgc caaaagcgtc ttgcgcgatt ttcgtaatgt
     4021 cttttgcaat cggctgttcc tctttggcgc agcaaccatc tctttaagct atatccccat
     4081 gatgagttgg gtggctgtct cgccggtgat cctgattgac gcaggcggct taacgacctc
     4141 gcagttcgcc tggacacagg ttccggtgtt cggcgcggtg attgtcgcga atgccatcgt
     4201 ggcgcgtttc gttaaagatc cgaccgaacc gcgctttatc tggcgtgccg tacccattca
     4261 actggttggc ctcgcgctgt tgattatcgg caatctgctg tcaccgcacg tctggctgtg
     4321 gtcgatgctg ggcaccagtc tgtatgcttt cgggattggt ttgattttcc cgaccttatt
     4381 ccgctttacg ctgttttcca ataacttacc gaaagggacc gtctccgcat cgctgaatat
     4441 ggtgatcctg atggtgatgt ctgtctcggt cgaaatcggc cgctggctgt ggtttaacgg
     4501 cggtcgcttg ccgtttcatc tgttagccgt cgtggcgggc gttatcgtcg ttttcaccct
     4561 ggcaggattg ctcaatcgcg tgcgccaaca tcaggctgcc gagctggcgg aggagcagtg
     4621 atttttgcgc gatcctgccg tcaggctcta ttcttaacgt tatgaataaa ctcattgaac
     4681 tcagacgcgc caaaatgttg gcgctctctt tactgcttat cgccgctgct acctttgtcg
     4741 ttacgctgtt tttgccgccc aatttttggg tgagcggcgt gaaggcgatt gctgaagcgg
     4801 cgatggtcgg cgcgctggcg gactggtttg cggtggtggc gctgtttcgc cgcgtgccga
     4861 taccgattat ttctcgtcat acggcgatta tcccgcgtaa taaagaccgg attggcgaaa
     4921 atctcggcca gttcgtgcag gaaaaatttc ttgatactca atccctggtg gcattgattc
     4981 gacgccacga accggcgttg ttgattggca actggtttag ccagccagaa aacgcccgcc
     5041 gcgttggtca gcatctgttg cagatcatga gtggttttct cgaattaact gatgatgcgc
     5101 gtattcagcg cctgcttaag cgcgcggtcc atcgggcgat tgataaggtc gatctttccg
     5161 gcaccagtgc gttgatgctg gagagtatga ccaaaaacga tcgtcatcag gtgctgctgg
     5221 atacgctgat cgcacagttg attgctcttc tccagcgcga taaatcgcgc aagtttatcg
     5281 cccagcaaat tgttcgctgg ctggagagtg agcatccact gaaagccaaa attctcccca
     5341 ccgaatggct gggcgaacat agcgcggagt tggtttctga cgcggtgaat tctttgcttg
     5401 atgatatcag ccgcgatcgt gcgcatcaga tccgccatgc gtttgatcgc gccacttttg
     5461 ccctgatcga caagctgaaa aacgatccgg aaatggcagc gcgagccgat gccgtaaaaa
     5521 gttatctgaa agaagatgaa gcttttaacc gctatctcag tgaattgtgg ggggatttac
     5581 gggaatggct gaaagcggat atcaacagtg aagattctcg tgtgaaagaa cgtatcgcgc
     5641 gggcgggtca atggtttggc gaaacgttaa ttgccgatga tgccttgcgg gcgtcgttaa
     5701 atggtcatct ggaacaagcc gcgcaccgcg tcgcgcctga gttttccgca ttcctgacgc
     5761 gccacatcag cgatacggta aaaagctggg atgcccggga tatgtcgcgg caaatagagt
     5821 taaatatcgg caaagatctg cagtttattc gtgtcaacgg tacgctggtt ggcggttgta
     5881 ttgggctaat tttatatttg ctgtcgcagc tcccggcctt gttccccctc ggcaattttt
     5941 agaaatccat gaaaaactaa gtggctaacc gcgagagaga tcaaataaac catactaaaa
     6001 gaatggtaat aatgtggcct cttttcatta tctcccgtgg tacggggaag gaaaatcatg
     6061 tcacttgtca ccgatctacc cgccattttc gatcagttct ctgaagctcg ccagaaaggc
     6121 tttctcaccg tcatggatct caaggagcgc ggcattccgc tggttggcac ttactgcacc
     6181 tttatgccgc aagagatccc gatggcagcc ggtgcggttg tggtttcgct ctgttccacc
     6241 tctgatgaaa ccattgaaga agcggagaaa gatctgccgc gcaacctctg cccgctgatt
     6301 aaaagcagct acggcttcgg caaaaccgat aaatgcccct acttctactt ttcggatctg
     6361 gtggtcggtg aaaccacctg cgacggcaaa aagaaaatgt atgaatacat ggcggagttt
     6421 aagcctgttc atgtgatgca gctgccgaac agcgttaagg acgatgcctc gcgtgcattg
     6481 tggaaagccg agatgctgcg cttgcaaaaa acggtagaag aacgttttgg gcacgagatt
     6541 agcgaagatg ctctgcgcga tgctattgcg ctgaaaaacc gcgaacgtcg cgcactggcc
     6601 aatttttatc atcttgggca gttaaatcct ccggcgctta gcggcagcga cattctgaaa
     6661 gtggtttacg gcgcaacctt ccggttcgat aaagaggcgt tgatcaatga actggacgcg
     6721 atgaccgccc gcattcgtca gcagcgggaa gaaggccagc gactggaccc gcgtccgcgc
     6781 attttaatca ccggctgccc gattggcggc gcagcagaga aagtggtgcg cgcgattgaa
     6841 gagaatggcg gctgggttgt cggttatgaa aactgcaccg gggcgaaagc gaccgagcaa
     6901 tgcgtggcag aaacgggcga tgtctacgac gcgctggcgg ataaatatct ggcgattggc
     6961 tgctcctgtg tttcgccgaa cgatcagcgc ctgaaaatgc tcagccagat ggtggaagaa
     7021 tatcaggtcg atggcgtagt tgatgtgatt ttgcaggcgt gccataccta cgcggtggaa
     7081 tcgctggcaa ttaaacgtca tgtgcgtcag cagcacaaca ttccttatat cgctattgaa
     7141 acagactact ccacctcaga tgtcgggcag ctcagtaccc gtgtcgcggc ctttattgag
     7201 atgctgtaag gagtggcagt gacatattcg attggcattg attctggttc taccgccacc
     7261 aaagggatct tactggcaga cggcgtgatt acgcgccgtt tcctcgttcc aacccccttt
     7321 cgcccggcaa cagcaattac tgaagcctgg gaaactctgc gcgaagggtt agagacaacg
     7381 ccgtttctga cgctcaccgg ctacgggcgg caactggtag attttgccga taaacaagtg
     7441 acggaaatct cctgtcacgg gctgggcgca cggtttcttg cgccagcaac gcgcgcggta
     7501 atcgacatcg gtggtcagga cagcaaagtg attcagcttg atgatgacgg taacctgtgc
     7561 gatttcctga tgaatgacaa atgcgcggcg ggcaccgggc gtttcctgga ggtgatctcg
     7621 cgcacgcttg gcaccagcgt cgagcaactc gactgcatta ccgaaaatgt cacgccgcac
     7681 gccatcacga gtatgtgcac agtgtttgct gaatcagaag tgatcagcct gcgctcagcg
     7741 ggcgtcgcgc cagaagcgat tctcgccgga gtgattaacg cgatggcgcg gcggagtgcc
     7801 aatttcattg ctcgtctctc ctgtgaagcg ccgattctgt ttactggtgg cgttagtcat
     7861 tgccagacgt ttgcccggat gctggaatct cacctgcgaa tgccggtaaa tacccatcct
     7921 gatgcgcaat ttgctggcgc aattggcgcg gcggtaattg gtcaacgagt gaggacacgc
     7981 cgatgaaaga gtttttattt ttatttcact ccacggtcgg cgtcatacaa acccgcaagg
     8041 cgttgcaggc agcgggcatg acttttcgcg tcagcgatat tccccgtgat ttacgcggcg
     8101 gctgcgggtt atgcatttgg ctgacctgtc cccccggcga ggaaatacaa tgggtgatcc
     8161 ctgggcaaac cgaagcgata tattgccagc agggcagcga ctggcagtgc gtggtgcatt
     8221 acgatgcaga agcgtcaacc aggcaatagg atctttaaat ataccaaccg gataattggc
     8281 tgcgcataat cctgcatgaa ggaagttgta gtaaatatct cgcctgccgt gaaatatttc
     8341 ccatccacaa aatgagcgat ttttgatagc accttactat tttacgtcta aaatataaaa
     8401 tactttttat tatacttaaa cccataatta tgcatagaga aataaaatga ttaccgggtg
     8461 agataatgat aaaagataag tttctgtgcg aagcttatct ttacccatcc ccctgattat
     8521 tttagattta gaagaacatt tcacaattca aggataaaaa tatgggctcc ctaaacatat
     8581 accaggatat tttcccaaca ctcggcatgt attcgggact aaaatcttgt catgaaaaaa
     8641 acaaccaacc atttgatatt aacacggaaa ttgaaaccat acaaaaacaa attaattatg
     8701 atataaatca tttgaatgat ggtttgatta agcgtgtact gaatcttttt attcacctta
     8761 tctctaatcc cgacaatctt gaattaacct taaatagata ttcatcaaca acagaacaaa
     8821 tcatcggcag aaccaaaaga aatggtttac atgagtttga cgttggcgat ctaaaaataa
     8881 tatttaatcg acaagatgat aatgaaagcg tattaactgt taaatataaa gataaagata
     8941 aagatataag tcatcactgc aatgttaaaa ccgagcaact gcagcagttt attaaaataa
     9001 tggaacaaaa agcgcaacta tcaatctata ttgacaagaa caatttgaaa gagagtattt
     9061 tctctgtttt gcacaatgac ccacaacacg tagataaaga tcaacacctt ccctgtgaaa
     9121 agtttttaaa acatgcctgc aaaagttcaa attcatttga agtgaaatta gatgccactc
     9181 atcaatatca acacctgaat aacttcatga tttcttttga cccagtagaa aatcaattaa
     9241 ccatacggga taacaataac aagactgaaa ctttctcgtt tacaaactta caatgggaaa
     9301 atttgctgca atactacaaa gaaaaccacc agcagccaaa tatagcagga tcacgaaatc
     9361 tcacggataa tatagataaa attaaaaata caatatccac ctctgaaatt attgaatgcg
     9421 cctctcctga aataggaagt agcgtcctga acgatcttta tagcattgct aatttcctcc
     9481 cggacaataa tctgacccca aatgagagct ggaaaagatt ttgtgacaca tgcgagcgct
     9541 tttacgttgc tcagaagagt atcactggag ataagagtga acgtcttacg cgaaaactct
     9601 ctatctctga tgcaggaatt acaatgacct tcaagatagg tgatgttgtc atcaatacta
     9661 ttatcactgc tattcctgaa gatgcaacgg gtcaacggtg tatcgaaggg ttgaatttag
     9721 cagaaatgga tttaaccggc atagacttgt ctaaaatggt tctaaggaat gtcaatttta
     9781 atggcagcat tcttagacat gccaagttct ccggtacgat ctgtgaaggc gtggatttta
     9841 cagattgtga tctgcgtaat gcaaaattta aaaatgcctc attagaaaat aatgattttc
     9901 gtaaagttcg ccacttgact tatgtaaatt tcaaaaacgc aaatctacga aacagtaact
     9961 tcagcggaaa agttctcact ggcgttaatt ttactggaag taaccttagc aacgcttatc
    10021 ttgaatatat agatttcaca acagtgattc tatatg
//
LOCUS       DAZFKF010000145         9971 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFKF010000145 DAZFKF010000000
VERSION     DAZFKF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.147"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..105
                     /gene="rrf"
                     /locus_tag="ACHKA1_004674"
     rRNA            <1..105
                     /gene="rrf"
                     /locus_tag="ACHKA1_004674"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            120..195
                     /locus_tag="ACHKA1_004675"
     tRNA            120..195
                     /locus_tag="ACHKA1_004675"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:153..155,aa:Thr,seq:ggt)
     gene            235..350
                     /gene="rrf"
                     /locus_tag="ACHKA1_004676"
     rRNA            235..350
                     /gene="rrf"
                     /locus_tag="ACHKA1_004676"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(413..1171)
                     /gene="yhdZ"
                     /locus_tag="ACHKA1_004677"
     CDS             complement(413..1171)
                     /gene="yhdZ"
                     /locus_tag="ACHKA1_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO5682229.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1179..2282)
                     /gene="yhdY"
                     /locus_tag="ACHKA1_004678"
     CDS             complement(1179..2282)
                     /gene="yhdY"
                     /locus_tag="ACHKA1_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO5682230.1"
                     /translation="MTKVLLSHPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2292..3473)
                     /gene="yhdX"
                     /locus_tag="ACHKA1_004679"
     CDS             complement(2292..3473)
                     /gene="yhdX"
                     /locus_tag="ACHKA1_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO5682231.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3541..4566)
                     /locus_tag="ACHKA1_004680"
     CDS             complement(3541..4566)
                     /locus_tag="ACHKA1_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5682232.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDICRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4997..5218)
                     /locus_tag="ACHKA1_004681"
     CDS             complement(4997..5218)
                     /locus_tag="ACHKA1_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682233.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5471..8582)
                     /gene="acrF"
                     /locus_tag="ACHKA1_004682"
                     /pseudo
     CDS             complement(5471..8582)
                     /gene="acrF"
                     /locus_tag="ACHKA1_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(8594..9751)
                     /gene="acrE"
                     /locus_tag="ACHKA1_004683"
     CDS             complement(8594..9751)
                     /gene="acrE"
                     /locus_tag="ACHKA1_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO5682234.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2644 a         2577 c         2408 g         2342 t
ORIGIN      
        1 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga
       61 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa atttagcgtg
      121 ctgatatggc tcagttggta gagcgcaccc ttggtaaggg tgaggtcccc agttcgactc
      181 tgggtatcag caccactttt taggttaaag ttcggcagat tagaaaagaa tttgtctggc
      241 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
      301 gccgatggta gtgtggggac tccccatgcg agagtaggga actgccagac atcaaatcaa
      361 gcgaaaggcc atccgaaagg atggccttta tgcttttcga actaacattc aattaatgga
      421 ttacctgcga taaaaatgcc ctcgtacgct ctgacttagg atgcgcaaaa aactcatccg
      481 gtgcagcttg ctccactatt tcgccacgat ccataaaaat cacccggtca gcgacggttc
      541 gcgcaaaccc catctcatgg gttacacaca acattgtcat acccgattgc gccagcccaa
      601 tcatcgtatc cagcacctct ttcaccatct ccggatcgag cgccgatgtg ggctcatcaa
      661 acaacataat tttcggcttc atacacagcg agcgcgcaat ggcaacgcgt tgctgctgac
      721 cacctgaaat ctgtccggga aacttatgcg catgttcggc aattctcacc cgctcaaggt
      781 aatgcatcgc cagagcttca gcctctttct ttggcatctt gcgtacccaa atcggtgcca
      841 gggtacagtt ctgtagaacg gtcagatgag ggaagagatt gaaatgctga aagaccattc
      901 ccacttcctg cctgacgcgc tcaatattgc ggatatcttc attaagttcg atgccatcta
      961 ccacgatccg tccctgttga tgttcttcca gatgattaat acaacgaatg gttgtcgatt
     1021 taccggaacc tgaagggcca cacagaacga tccgttctcc cggttgcacg gttaaattta
     1081 tatttttcaa aacatggaat tgtccatacc atttattgac gttttccagc gtaatcatcg
     1141 cgttagcagg ttgcagtaaa atttggctca tagtgtcctc aatgcggtgt acgcccggtg
     1201 ttaaaacgtt tttccagatg ctggctatag cgcgacatgc tgaaacagaa gatccagtag
     1261 atcagcgcgg cgaaaacata cccttccgtc gacataccca accaggcggg atcaacggtt
     1321 gcctgctgaa cgctactgaa aagatcgaac aacccgataa tgatcaccag gctggtatct
     1381 ttgaagagtg cgatgatggt atttaccagc ccaggaatta ccagcttcaa cgcctgtggc
     1441 agaataacca gcccctgagt tttccagtaa cccaacgcca gcgactctgc cgcttcatat
     1501 tgccctttag gcagcgcctg taatccacct cgcacgactt ccgcaacata tgctgactga
     1561 aacaggatca cgccaaccag cgcgcggatc agtttgtcga tactggtgcc ttctgccata
     1621 aacaaaggca gcatgaccga agacataaac agaacggtaa tcagcggcac gccgcgccag
     1681 aattcgataa aaatgaccga taagatacgc acaatcggca tatgggagcg gcgacctaac
     1741 gccagtaaga tcccccacgg tagcgccccc gcaatcccaa ctgatgcaat aattaaagtt
     1801 agcgtcagcc cgccccattg ccgggtttca acccgctcaa gagcaaaaaa accgccatac
     1861 atcagccacc agacaatcag tgggtaaatc actgcccaga ccgcaatata gcgaccgcga
     1921 tgcgggagta ttttccagaa cattggtgcg attgacacaa ggccaatcag taatgccagg
     1981 ttaattcgcc agcgttggtc gtgtgggtaa agtccataca taaactgacc aaatcgttcg
     2041 tggatgaaga cccaacaggc accggctttt gtgcagtcgg cacgcgttga gccaacccag
     2101 ttagcctgca aaaatgccca gttcagcaac ggtgggatca gttcccacat caaccatatg
     2161 cagccaatag tcagcaggct attgctccag ctggagaaca gattttttcg cacccacacc
     2221 atcgcgcggc ttgagttatg gctcgccggg cgcgagggat gagacagcaa tacttttgtc
     2281 atcatgactc cttagcgttc cacgatcgcg atgcggcggt tatagatatt catcagcagc
     2341 gagatagtca ggctgataat cagatagacc gacatggtca tggctatcgt ctcgatggct
     2401 tgccccgtct ggttcagcac ggtgccagca aacagcgaaa ccatatcggg atagccaata
     2461 gcggcggcaa gagaggagtt tttgacgatg ttgagatact ggctggttaa cggtggaata
     2521 atcacccgca gtgcctgggg aataatgacc tggcgtagcg taaccgggtt gggtaatccc
     2581 agtgaccgag ccgcttcatg ttgaccataa ggcactgcct ggatccccgc gcggataatc
     2641 tcggcgataa atgcagaggt ataaaccgaa agtgccagcg ttaaggctgc cagttcagga
     2701 attaaaacca tcccgccgcg gaagttaaag ccttgtaggg ctgggacatc ccagtgtagt
     2761 gctgcgccaa aaagccattg cgccagtaaa ggcaaaccga tgatcaacac tgcggcgatg
     2821 ggccaggtgc ggcgcagctg tccggtcttt atctggtatg ttttattaaa gcggaatagc
     2881 ccaacagaaa ggactatagc cataacaaca gccaggataa acgcaataaa tccgtctccc
     2941 agctgcggtg acggaatata aagcccacga ttgctcaaaa aggccagatc aaacgcgctc
     3001 acagcctggc gtggtccggg caaattgcgc aacacggcaa agtaccagaa gaagatttgc
     3061 agcagcgggg gaatattacg gaagatctca atataaattg tggaaagctt tcgtagcagc
     3121 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     3181 cacaatgcag aaaccagtag cgtattaagt aagccgacaa taaaaacgcg tccgtaggtg
     3241 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     3301 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt gtgaaataac
     3361 caaccaacaa tgccgacaac agcaacaacg gcaaggatct ggaataacca ggcacgaacc
     3421 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3481 tcggaaagca taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac
     3541 ttaacgcacg ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3601 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3661 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3721 gccttcttta cccagcagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3781 gacgttctgg gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg
     3841 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3901 gataacctcc ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcagggcata
     3961 cagttgcgac tgatccgagg ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     4021 ttcgtcagag cgatcgaaag tcaccggtgt gtacttcata ttgtttgcct tgaaatagtc
     4081 ggcaacgttg agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc
     4141 tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt
     4201 gacgccagta aatgccattc ccatcccggc gtcgcgagat gaagtccagg tcgtattacg
     4261 ggagagcaaa tccacttccc ctgactgtaa agccgtgaag cgttcttttg ctgtgagtgg
     4321 ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaatatc
     4381 aacatcaata cctgaaaatt taccgtcagc atcggcataa gagaacccag gtaatccatc
     4441 actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg
     4501 cgcctgattt gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt
     4561 tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt
     4621 tacctgtctg tggccatcct cttataagca aaagtagtgc catgttttgc gcacgatttc
     4681 gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtgcg
     4741 aaaatgaaac atacgccaca ttttggcgca aaatatttac gcgtgtttta ctaagcagaa
     4801 taatgattgt tagcgtgagt atatttctgg tgggaagtgc gaaagttaaa ttgatgcgtt
     4861 atttcgaaag cagcgaaata acgcgatata acatgctaaa tcagcagaga attagccagc
     4921 agaatatagc aatattttat cttatgtaaa gatactgtat cgttaaacag agcgcgaaac
     4981 tccgcgccct gttcagttat ttcgacagag cgatgatccc catcgtcagg ccagcaacag
     5041 ctgctgctcc gccagcaata gccccagccg tttcccagtt atcctgagtt gtgcctttca
     5101 tacaggtatt ggtgtgaacc agacgcccct gatcgtcata aaccggaacg cagggcgaat
     5161 cgtgagcaca gcccgccagc aatgcggtga gcaatgccac aggaattaat cttttcatgg
     5221 tgttacctca gtaataaacg ctaacgctta ataagttaac tctcttatta acgagctgaa
     5281 tgccatttta tcaccggagg ttataaatct tacggaatga ataatgacaa attatgtgaa
     5341 ttgtaaaaat agtgaaggga gcgggaacta tagaaagttt ttaaagagtg agtggaagaa
     5401 tatcttatta atttttatgg gtttcactgg aaataataaa ggcgcccgaa agcgccttta
     5461 tgtttctgat ttatccttta aagcaacggc ggatcaccac aaagaacact ggtacgaaga
     5521 agattgccag caacgttgca gagaccattc cgcccattac cccgataccc actgcgttct
     5581 gcgcgccact gccggcaccg ttactgatag ccagcggtaa tacgccgaga ataaaggcga
     5641 gagaggtcat caggatagga cgcagacgca tacgtactgc cattagtgtc gcttcaacaa
     5701 cacctttacc ctctttctcc atgagatctt tagcgaactc aacaatcaaa atagcgtttt
     5761 tggccgacaa gccaattgtc gttagcaagc ccaccataaa gtagacgtca tttttttgat
     5821 taaagagtgt cgccgccagc agcacgccga caatccctaa cggcacgacc aacataaccg
     5881 agacaggaat tgaccagctt tcatagagtg cagcaaggca caggaaaaca accacaaagg
     5941 aaattgctac cagagcggga gcctggtttc ccgataagcg ttcctgatac gacatacccg
     6001 tccagtcata accaatgccc gcaggtaatt ttgacgcaag gttttccatc aacgccatgg
     6061 catcgccgga actggttcct ggcgcggctt ccccctgaat ctccattgac ggcagaccgt
     6121 tgtagcgttc cagtcgcgga gagccataca cccaatgtga agtggtaaag gcagagaatg
     6181 gcaccatttc gccgttggcg ctgcggacat aaagtttatc gacatcttct ggcagcatac
     6241 ggaatttggc atccgcctga acatacacct ttttcacgcg accacggtcg atgaagtcgt
     6301 taacgtaagt cccacccagc gccgttgaaa tggtctgatt gatgtcagaa agtgaaacac
     6361 ctaatgcctg tgccttttcc tggtcaactt ccagtttaaa ctgcgcggtg tcttccaggc
     6421 cattaggacg cacgctgact aagctggcag gatgttgcgc cgccatacca agcaactggt
     6481 tacgggcctg ggttagggca tcgtgaccca gcccagcctg atcaattaac tcaaagtcga
     6541 aacccgttgc cgtgcccagt tcaacaatgg ctggcatgtt gaatggaatg acaaaaccgt
     6601 cgcggatctt gcccaattcc attttggcac gatggattac cgcttccgca ctgttttcgt
     6661 caccattacg ctcttcccac ggtttcagac tgacgaaggc cataccggcg ttttgtgcct
     6721 ggccgctgaa gctaaagccg ttaaccgtaa agacactttc aacgttcgct ttctcgttct
     6781 tcagatagta atccgtcact tgatccaaca ctttttgcgt ccgctcttgc gtcgcgccag
     6841 cgggtaactg aatcatggtc agaaagacac cctgatcctc ttcaggtaag aaggaagacg
     6901 gaagacggaa gacgtaaaaa caacactacc attcctgcaa caatcagcgc atagatcagt
     6961 aaatatcgtc ctgtggaacc gaggattttg ccgacgctgt tggtgtagtg gttaacgcta
     7021 tgatcgaagg tggtattaaa ccagccgaag aaaccgccct tattttcgtg atgctcagca
     7081 gagacgggtt taagcagcgt tgcacataac gcaggggtaa gaatcaatgc caccagaaca
     7141 gaaagcgcca ttgccgaaac gatggtgata gagaactggc gataaattgc cccagtagaa
     7201 ccgccgaaga atgccatcgg aataaatacc gctgacagca ccatcgcgat acccaccagt
     7261 gcgccctgaa tttgcgacat tgatttttcc gtcgcttctt ttggcgggag cttatcctcc
     7321 atcatcacgc gctcgacgtt ctccaccacc actatcgcat catcgacgag cagcccgatg
     7381 gcaagcacca tcccgaacat cgttagtgtg ttgatggagt aaccaaaagc ggcgaggatg
     7441 gcaaacgtcc ctaacaacac cacgggtacc gcaatggtgg ggatcagcgt tgctcgcata
     7501 ttctgcaaga acagatacat caccaggaac accagcataa tagcttcgaa cagtgttttt
     7561 accacttcgt gaatagaaag ctggacgaat ggcgtggtgt cataagggta gagaaccttc
     7621 attccctgcg ggaagaatgg ctgtaattcc gccagttttg ccttaatggc tttcgcggta
     7681 tcgagagcgt tcgcaccggt tgccagctta atacccaggc ccgccgccgg ttttccgttg
     7741 atgcgagcga taacgttata gttttcaccg ccaagttcaa cccgtgcgac atctttcaga
     7801 cgcaccaccg agccgtcact gtttacgcgc agggtcactt tgccgaattc ttccggattt
     7861 tttaaccgcg tctgagcaat aatcgaggca ttcaactgtt gccctggtaa cgctggcgtt
     7921 ccgcccaact gtccggcagc gatctgatcg ttctgtacct tcaactggtt aatcacatca
     7981 accggtgtca gtttatattt gtttagcaga tcggcgtcca gccagatacg catcgcatac
     8041 tgtgcgccga aaagctgtac gtcaccgacg ccattcagac ggctaagcgt atctttaacg
     8101 ttagaggcca catagtccga gatatcgtcc tgtgtggtgt ctgggttatc agagacaaag
     8161 cccgccacca tcaaatagct gctactggac ttttcaacac tgatcccctg ctgctgaacc
     8221 tcctgcggca gcaacggcgt ggcgagctgg agtttgttct gcacctgcac ttgcgcgata
     8281 tcaggatcgg tcccggactg gaaggtaagg gtaattgtca cgctaccggc ggaatcgctg
     8341 gtggaggaca tatacatcag gttatcgata ccgttcatat tctgttcgat aacctgcgtc
     8401 accgtatcct gcacggtctg cgcatcagcg cccggatagt ttgctgaaac agaaaccgca
     8461 ggcggtgcaa tggttggata ctgagcgacg ggcaattgta ggatcgccag tgcgcccgcc
     8521 atcatcagaa taatggccag cacccatgca aatattggtc gtcgaataaa aaagtttgcc
     8581 atgtcagatt accttacttc gatgcagtat ctgcgggggt atcggtagtg gctttaacct
     8641 gctctcccgg acgcgctttt tgcaggccgc tgacaatgac ttgatcgcca gatttcagtc
     8701 cttcactaat caaccattta tcgccaatcg cctgactggc aacgacaggg cgcgcttcaa
     8761 cctgactttt atcgttaaca atcagcacgg ttgcatcacc acgcggtgtg cggctaacgc
     8821 cttgttgcgg gataagaatg gcgtcaggtt ggacgccttc atcaatccgt gcacgcacaa
     8881 acatacccgg caaaagcgta tgttgcgggt tagggaagac agcacgtagg gttatggagc
     8941 cggtgctttc atcaacggtc acatcggaga attgcagcgt acctttcagg ggataggttt
     9001 gaccgttttc catgaccaac tctacgttgc tggtggcgtt ttccttatgc aaatttcctt
     9061 gctctactga ttgcttcagc ctcataaaat cgttgctgga ttgggtcaca tcaacgtaga
     9121 taggatcgag ctgctggaca gtcgccagtt cagtcgtttg cccattagtg acaagagcgc
     9181 cttcggtcac agtcgatttg ccgatacgtc cgctaattgg cgcagtcact ttggtataag
     9241 caagattgat gcgagcgctt tcgactgtgg cttttgcggc aatcacggcg gcatcggcct
     9301 ggcgagcatc agcaatggcc tggtcgtact cctgctggct gatgtatttc gtacccacga
     9361 gcggaacgta acgttttacc gtcaaatgcg cgatggcggc ggcggcttca cttttcgcca
     9421 gttcgccttt cgcgctgtca taatttgcct gataggtcgc gggatcgatc tggtacaggg
     9481 actggcctgc ttgcacatcg ctgccttcag tgaaattgcg attcagtacg atcccgctaa
     9541 cctgtgggcg aacttcggct atacgataag cattggtgcg gcctggtaat tcagtcttaa
     9601 cttctaacgg ggccgttttt acaatatgaa cggtaacctg cggttcaccg acgtgagctt
     9661 tctcttctcc cttatcgtta caaccggcga ttaaagccgc ggagatcaga ataaaggagg
     9721 gcaggaggaa aaacctggca tgtttcgtca ttactattcc tcaaaaaacc aaaagcgcgt
     9781 tatttaccca aaaaggcaac gcgttaactc gcagaaagaa aaatacagtt cgctatccta
     9841 caaattatca ttcgtcgatg taaggaatag ttatgaatac aggcgtctca aggcacataa
     9901 acacaaaaaa agattaatat tctactgttt tattttgacg cgggttgaaa gaggcagaat
     9961 taaaacctcg t
//
LOCUS       DAZFKF010000146         9884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFKF010000146 DAZFKF010000000
VERSION     DAZFKF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.148"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..157)
                     /locus_tag="ACHKA1_004684"
     CDS             complement(<1..157)
                     /locus_tag="ACHKA1_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682235.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKISKMMNGKLIYSWLKWQCG
                     EFFFLIIS"
     gene            235..1329
                     /gene="ddlA"
                     /locus_tag="ACHKA1_004685"
     CDS             235..1329
                     /gene="ddlA"
                     /locus_tag="ACHKA1_004685"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO5682236.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1353..1565)
                     /gene="yaiZ"
                     /locus_tag="ACHKA1_004686"
     CDS             complement(1353..1565)
                     /gene="yaiZ"
                     /locus_tag="ACHKA1_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO5682237.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1825..2133
                     /gene="yaiY"
                     /locus_tag="ACHKA1_004687"
     CDS             1825..2133
                     /gene="yaiY"
                     /locus_tag="ACHKA1_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO5682238.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2192..3286)
                     /gene="yaiW"
                     /locus_tag="ACHKA1_004688"
     CDS             complement(2192..3286)
                     /gene="yaiW"
                     /locus_tag="ACHKA1_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO5682239.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSRAPQPLKKGEKEIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPPFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3299..4519)
                     /gene="sbmA"
                     /locus_tag="ACHKA1_004689"
     CDS             complement(3299..4519)
                     /gene="sbmA"
                     /locus_tag="ACHKA1_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO5682240.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4871..6028
                     /gene="ampH"
                     /locus_tag="ACHKA1_004690"
     CDS             4871..6028
                     /gene="ampH"
                     /locus_tag="ACHKA1_004690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO5682241.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6029..6652)
                     /gene="iprA"
                     /locus_tag="ACHKA1_004691"
     CDS             complement(6029..6652)
                     /gene="iprA"
                     /locus_tag="ACHKA1_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO5682242.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6740..9637)
                     /locus_tag="ACHKA1_004692"
     CDS             complement(6740..9637)
                     /locus_tag="ACHKA1_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5682243.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYVDTNKPIYN
                     IDTDYLTYSGYVYNTVAKDSGYYDTVFSGDTVNGVISTYYLNYDDYAAGTANTVSINN
                     STVHGMITSKYVPNFGDAAKAHDWIDGDTFTLNISNSTIDDDYEGFYFTDTYLDADGK
                     TVKSDNYEYSVAGMGTAVTLDVESNINISNNSHVAGITLEQNDTTNTAYKTGATQWDN
                     TITVNDSTVTSGSWTEDEQQDGHFGNSAEPSDYSGDGRVGYHSNDNALEFIDDSSSDY
                     RMVNNVTFNNSQLMGDVYFQSTWNNNFDYIGHPTDTDSNVYTHGGWIDDDQNVDHLTL
                     TLNHTKWVGGAYIDYAGDDIVTPDHFYDVAANSLDPNATNYDGWNRMDSAASFQSGVF
                     DVVLNDGSEWDVAKDSRIDTLAVNNSQVDVANGVELTSDTIGLNDGSVLNIGEGGQVN
                     TDHLTVNTFSTVTLANDISSDWALDALYANTITVTNGGVLDVQTSNTDAANVFTTDTL
                     ELTSGNVKDNHGNVYAGVFDINSNDYILNADLVNDRTNDTSKANYGYGVIAMNSDGHL
                     TVNGNGDINNVDTAATNQGGEADNSTADNVVAATGNYKVRIDNSTGAGAIADYAGKQL
                     IYIDDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNI
                     WNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKI
                     DGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFN
                     NDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSISGLFQSGDDYQL
                     SNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSI
                     DNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2564 a         2442 c         2339 g         2539 t
ORIGIN      
        1 aacttataat taagaaaaaa aactcaccgc attgccattt aagccaggaa taaattagct
       61 tcccgttcat catttttgag atcttacgca catctttgag ataacgcact ttcttcacca
      121 gtacgttgca cgctttgtca gcagaagatt gcaacaatct ttcagaaggc cgcgaatccc
      181 ccgttttgtc gtggtaatgt atgcacttta tgttagacgg gataggtttt taagatggaa
      241 aaactgcggg taggaatcgt ttttggtggt aaatcagcgg aacatgaagt gtctctgcaa
      301 tcggcaaaaa acattgtcga tgccattgat aaaagtcgct tcgacgttgt gctgctgggc
      361 attgataaac aagggcaatg gcacgtcagc gatgccagca attatctgct aaatgcagac
      421 gatcctgccc atattgcgtt gcgcccttcg gcgaccagcc tcgcccaggt gccaggtaaa
      481 catgagcatc agcttatcga cgcgcaaaac ggtcagccgt tgccgacggt ggatgtcatt
      541 ttcccgattg tccacggtac gctgggcgaa gatggttcct tgcagggaat gctgcgggtc
      601 gccaatttac cgtttgtagg ttctgatgtt ctggcttcag cagcctgtat ggataaagat
      661 gtcaccaaac gtctgctacg cgatgccggg ctgaacattg cgccatttat taccctgacg
      721 cgcgctaatc gtcacaacat cagttttgcc gaagtggagt ctaaactggg gttaccactg
      781 tttgtaaaac cggctaatca gggctcttct gttggtgtca gcaaagtaac cagtgaagaa
      841 cagtacgcaa ttgccgtcga tctggcgttc gagttcgatc ataaagtgat cgttgagcaa
      901 gggatcaaag gtcgtgagat cgaatgcgca gttctgggca acgacaatcc gcaagccagc
      961 acctgtggcg agatcgtact caccagcgat ttctatgcct acgacaccaa gtacattgac
     1021 gaagatggcg cgaaagtggt agttccggca gccattgcgc cagaaatcaa cgataagatc
     1081 cgggcgattg ccgttcaggc ttatcagacg ttgggatgcg caggcatggc gcgtgtagac
     1141 gtgtttttaa ccccagagaa cgaagtggtg atcaacgaga tcaacacact gcctggcttc
     1201 accaacatca gtatgtatcc gaagctgtgg caagccagcg gtctgggtta caccgatctg
     1261 atcacacgtt tgattgagct ggcgctggag cgtcacgctg cggataacgc attgaaaacc
     1321 acaatgtaat attttaacgc ccgataatat tcttattggg cgttttcagt ttcttcatcg
     1381 cgacgacgaa taatcaaccc cgccagccag aaactaatca cccacgtcac cagaccgacg
     1441 gcataggtct gccaaccttt tgcttcaaat cccagtaacc ccaccacgcc attaagaatg
     1501 aatataaggc caatggcgaa cgcatagtag tgccagtcac ggcggatttt tacaggcaga
     1561 ttcatggctg ctccgttaac gaaaaacaac atccttgcat attctgcaac aaccgtctgt
     1621 ggtgtagaca acattttcgc gatagttaat aaatttaact taaagttgct attttgtgac
     1681 tcgcaacgaa gattcacgct tcgggaatat tcccaacatc aatttaccat gattctgata
     1741 ttgacaatct aaatgacctg tcagacttgc cataagccgc tggtatcaca ccagaagaga
     1801 agcagatgac ttccggaggt ttttatggct gatttcaccc tgtcaaaatc gctgtttagc
     1861 ggaaaatatc gcaatgcctc ttcaacgcct ggcaacattg cctatgcgtt gtttgtgctg
     1921 ttttgctttt gggctggggc gcaattgctg aacctgttag tgcatgcgcc cggcgtctat
     1981 gagcgtttaa tgcaggtcca ggaaacaggt cgcccacggg tggaaattgg tttaggtgtc
     2041 ggcaccattt tcgggctgat cccgttttta gtaggctgcc tcatttttgc agtagtggcg
     2101 ctatggctgc actggcgaca tcgccgccag taagatttac gttgccagat acggcctaaa
     2161 acgccttatc tggcatgaca ttcctgtttg atcactgttt catacaacgc gcccgccgtt
     2221 cgtctacgcg cttcgcaaac caggccgtag tcaggttgcg ggtgattttc gggctttcca
     2281 gttgaatgcc aggcaacatt tcgcggggga gtgatttacc cgttttcgct tcggcaagtt
     2341 gataaacttt cttgtacagc gccgtctcct caaaagagaa gctgtcacct ttctctagct
     2401 gacgacgaat ttcgctgtcg ctcatcccca gttttgcagc cagtttgcgc gttgccagtt
     2461 cggttttccc gggttcttta ctgtcgtaac gaattaaatc accgtccagt gccagcttca
     2521 cgccgctggc cttactgacc gcgttctgaa acgcggcatt acggctggcg taccagccag
     2581 cgttaaaatc agcaaagcgg tatatcggtg cgctatagct ggcgggataa ttcagtaaat
     2641 ggtaagtacc aaaccacaac ccgccgcggc gactgaagac ttcctgacgg actgtaccgt
     2701 ccattttcca cggataccct ttggtatgct gttcggcaaa agcaatgctg acctgcatcg
     2761 gcccaccggt gcgcaccgga ttaagcgaac caaacagcgt ctgccccatt ggcaccatgc
     2821 tgatcagatc gtcaaaaatc gcgctcaatt gcttttctgt acgcaccgaa tctaaccgtt
     2881 cgctataact tttgccgttt ggcgatttga tttttagcgc ggtatgaacc agaaaaggag
     2941 gaatgtgcat ccgttcagca cgacggtcaa tctcttgcca ggcgattttg cttaagcccg
     3001 gaacggccgg atctgcctga taatttgact cctgctgcgc caccgccagc accgagcaga
     3061 cgttttccag cgtcggtgcc agcccctgac tttcaaaggt ggtggcgaga tctttcgccc
     3121 aggcatcgcg gtctttcacg ctcgcaggca tcttctgccg cacgacgctc gccacatcta
     3181 tctctttttc gccctttttc aacggttgcg gagcccgact actacacccc gctaaaacca
     3241 gaaccgccaa caatgacagc gaagatagag gattgacgcg cgacattact tctccttttt
     3301 agctcaaggt atgggttact tcctgaattt tgtcaccatc cagctcatgt tcaaagctgc
     3361 gcagacgttt gtagatagac atcaactcaa ccagtgtggt ccatgagtta atcaggtact
     3421 ggaaagcacc gcgaacctga ccaaaaacgt tggtaatctg cgtcatcagg ccgagcgtaa
     3481 tcgtaccggc aacaattgac ggaaacagca agaacaaacc gaaaacgtta tcgacctgca
     3541 aatagagaat gcgagcgatg ttgaaataca tatagtgaaa atagaggcgg aaatagtttt
     3601 tccgtacggc gctaaacagc tcgcgtaccg ttggcggcgt cgcgcgcgtg gcatcgtctt
     3661 caccataaac cagctcttta cggtaggcag cctctactcg ctggttttta aactccagcc
     3721 ccggcagttt gatccctacc actgccagca atccggtccc catcagcgac cagacgatag
     3781 cggcaatcac cagaccatac ggaatgtgcc cgataatcgg cagctccggc acatgcgcgg
     3841 agagcgttac cagcaccggc aggaaggcga tcaacgtcat gatggcgttg ataaaactga
     3901 cacccatatt ctccagcgtt gaagcaaaac gcatggtgtc ttcctgcaca cgctgtgcgg
     3961 cgccttcgat atgacgcagt tgttgccagt tcgccatgta atattcgttc atcgccgtac
     4021 gccagcggaa cacgtagtga ctgacaaaga agttattcag cacactgatc actaccgcga
     4081 tcagcgcaat ccccagaaag acgcccactt cgcggtaaaa ttgttcgata gtgactttat
     4141 gcggcgaact tagcgcggtt tgaatcagat catagaacgg tgcataccag gcgttgacgg
     4201 caaccccgac ttccaccaaa aaccaggtga cgaagatgat cagtgcagta ccgagtattg
     4261 accagtattg ccaacggtgc gggctgtaga taaaccagaa caatgcaaaa agtcctacgc
     4321 aaacaatgta gtaagcgtaa aaaatcagga aatccaacga ccagaaacgc gcggcgctaa
     4381 tcgggatctg cccggaagcg ccggtgatac gcgccaccca gtcaccccca ccggcttgcc
     4441 agaagataac ggcaatcaat gcccaaacaa aggccgagag aaaaaacgtt cccggctttg
     4501 ggaaaaaaga cttaaacata tgcactcctg ctaacttctt atcgttttaa caactgctgt
     4561 gtattaaccg catgaccgct acaaagcgtc agccaaatga aaccgcccat tgtgacccat
     4621 agcaaggcgc ttagttcgcc agcctggcca taagaattgt ttcgacggat ttcacaaacc
     4681 aaagattgtt tacataagcg gattaacacg cgcaggatca caaatgcagg cattggtgag
     4741 caggaaaaag atcgtgttta cgcgccgtta cgtttttaac actattattg cactaattcg
     4801 ccctttgcaa tccatcaatg agtagtataa atacgctcag ttaccttcat tcaatttatg
     4861 gacaccaccg ttgaaacgta gtctgctttt ttctgccgtg ctgtgtgcgg cgtcattgac
     4921 ctccgtccat gcggcgcagc cgatcactga accggagttt gcctctgata ttgtcgatcg
     4981 ttatgccgat catatttttt acggcagcgg tgccacgggg atggcgctgg ttgttatcga
     5041 cggtaaccag cgcgtctttc gcagttatgg cgaaacgcga cctggtaata acgttcgccc
     5101 gcagctggat tcggtcgtgc gtattgcttc cctcaccaag ctaatgacca gtgaaatgct
     5161 ggtgaaattg ctcgaccagg ggaccgtgaa gttaaacgat ccgctaagta aatatgctcc
     5221 gccaggcgca cgcgtgccaa cctacaatgg gacgcccatt acgctggtca atctggcaac
     5281 ccataccagc gccctgcccc gtgaacagcc cggtggcgcg gcacatcgtc cggtgtttgt
     5341 ctggccaacg cgcgagcaac gctggaaata cctttctacg gcgaagctga aagccgcgcc
     5401 aggcagtcag gcggcgtact ctaaccttgc gttcgacctg ctggcagatg cactggcgaa
     5461 tgcgtccggc aagccttata cccagttgtt tgaagagcag attacccgtc cattggggat
     5521 gaaagacacc acctacaccc cctcaccgga tcagtgccgc cgtttaatgg ttgcagagcg
     5581 cggtgccagt ccatgcaata acacgctggc ggcaattggc agcggcggcg tctattccac
     5641 gcctggcgat atgatgcgct ggatgcagca gtatttgtca tctgattttt atcagcgcag
     5701 taaccaggca gatcgcatgc agacgttgat ttatcagcgc gcgcagttta cgaaagtgat
     5761 tggcatggat gtccccggca aagcggatgc gcttggttta ggctgggtat atatggcccc
     5821 gaaagagggt cgtccgggga ttattcagaa gacaggcggt ggtggcggtt tcattaccta
     5881 tatggcgatg atcccgcaga agaatattgg cgcgtttgtg gtggtcactc gttcgccgtt
     5941 gacgcgcttt aaaaatatga gcgatggcat caatgatctg gtgaccgagc taagcgggaa
     6001 taaaccgttg gttatccccg catcctgatt aatactctga aggcaaacgg ttgatagcga
     6061 ccagtttgcc tttattcatt tcgatatagc cgcctttacg caaagcagcg agaacttctg
     6121 cgacgacaga acgcgatatg cgtgtacgtt gatggatata attcatcaca ccaatacgtg
     6181 atcgcaattc ttcattccag tcaatcattg ataataatgt tgcgcggatt tgttcgtagg
     6241 aattatgccc aatgagctgc acgtcgcgta attccagaat tctattttgc caggctaacc
     6301 agtaaaaagc gtctcgccag agttgatttt gttcaataag cgtaatggtt tgtttggctg
     6361 gtagatgata tcccgtacaa tttccttctg atattaattt gtatggtata tcgtttttca
     6421 ttaaaccatc agccagcccc ataatataag gagcctgggt aataccgata agtacgtttt
     6481 cttctctacg cagagagata acgccctcta aaataacgaa agtatcttcg ttattgacat
     6541 cactggaaaa tataacttgc ttttcattat taaactcgaa gcgcgtaccg tatctggaca
     6601 aacatttatc gagcttacca aattcctgaa gaggtttaac tacagataac atttgcgcgt
     6661 cctttgcagt aatgcccgtc aaatccttga cgggcattat ttagattaaa ttaccagaat
     6721 ttcttcggag tgaagaatat taccaggtat atttaacacc cacgttcgcg gaccaatctt
     6781 gatctacgtc accaccaccg aggtagttag catcggtata ggcgctgaag ttcttggtga
     6841 agctgaactg agtacccaga ccaacacgta ccgcagaccc ttcagtaccg ttatcgatgg
     6901 aatcaccgtt cacatcgtta tcgttgttag agtcgtcgta gacgtaagcc agtttgaagt
     6961 acggagtcag agcctggtct tcgctgtaag tgaaggtata acctgcatct acacccagtt
     7021 cataacgcat gctgtcgtaa gactgaccgt caactttcat gtcgttgctc agctggtagt
     7081 catcaccaga ctggaacaga ccagaaatgc tgccgtaagg agtcacgtaa ccagcatcac
     7141 ccagtttgaa gtcgtaaccg gctttcaaac cgaagcccca agcgtcggag ttggtgctac
     7201 cgtcaacgta agtaccgttg ctcatggttg cagacaggtc gttgttgaag tgagagtagc
     7261 tcaagctacc atcaacaaag acgttgttcg cgaagtgagc agaagagtag atgtaggcag
     7321 tctggctgtc ttgatccacc tgaccagaac ggtcattcat gtcaccttta gcgaagcctg
     7381 cagccgcacc gacgatccac ttagcgttgt taccgtcaat tttggtatca acaccgacca
     7441 tgatgccgtt aacatcctga tcatagttga tggtgccgtt gtcaccgttg aagttaccac
     7501 cgaagtagct tacccatgcg ccgccgttat cagccaggcc atgacgagag ttggtcaaac
     7561 gagtaccaac ggtgtcttgt tccaggttcc agatattggt gttcgcagac gggatgctca
     7621 gcgccatgtt agcgtagtca gtcagctcca tctgttgcag aacaacggtg ttaccgcgct
     7681 gttcagcctg ataggtgtat gcacccaggt cagctttgtt agcagcagag aaggtcgcgt
     7741 tggttttggt atcatcgata tagatgagct gcttacctgc gtaatcagca atcgcaccag
     7801 cgccagtgga gttgtcgata cgaactttgt agttaccggt tgcagctaca acgttgtctg
     7861 cagtgctgtt atcggcttca ccaccctggt tggttgcagc ggtatcaacg ttgttgatat
     7921 caccattacc attaacagtc aggtgaccgt cagagttcat tgcgattaca ccgtaaccgt
     7981 agttagcttt gctggtgtcg ttggtacgat cgttgaccag atccgcattc aagatgtagt
     8041 cgttgctgtt gatatcgaac acaccagcat aaacattacc atggttgtct ttcacgttac
     8101 cgctggtcag ttccagagta tcggtggtga agacgtttgc agcgtcagta ttactggtct
     8161 gcacatccaa tacaccgccg ttagttacgg tgatggtgtt ggcgtacagt gcgtcgagtg
     8221 cccaatcgct gctaatatca ttagctaagg tgacggtact gaaggtgtta acagtcaagt
     8281 gatctgtatt aacctgacca ccttcaccga tgttcaaaac agaaccgtca ttcaggccga
     8341 tagtgtcaga cgtcagctca acaccgttag caacatcaac ctgagagttg tttacagcca
     8401 gggtatcaat gcgagagtct ttcgccacat cccattctga accatcgttc agaacaacat
     8461 caaacacgcc gctctggaaa gaagctgcgc tgtccatacg gttccaacca tcatagttag
     8521 tagcgtttgg atccaagctg ttagccgcaa cgtcatagaa atgatccgga gttacgatgt
     8581 catcaccagc ataatcaata tacgcaccac cgacccattt agtatggttc agggtcaggg
     8641 tcagatgatc gacgttctga tcatcatcaa tccatccacc gtgggtatag acgttggaat
     8701 ctgtatcagt tggatgaccg atgtaatcaa agttattatt ccaggtactc tggaaataaa
     8761 catcacccat caactgagag ttgttgaagg taacattgtt gaccatgcgg taatcagaag
     8821 aactgtcatc aatgaactct aaagcgttat cattgctgtg gtaaccgaca cggccatcac
     8881 cagagtaatc actcggctca gcagaattac caaagtgacc atcctgctgc tcgtcttcgg
     8941 tccaagaacc agaagttaca gttgagtcat taacagtgat ggtgttgtcc cactgagttg
     9001 cgccagtttt gtacgcagtg ttagtggtat cgttctgttc cagcgtaata cctgcaacat
     9061 gagagttgtt gctgatatta atgttgcttt caacatccag cgttactgcg gtacccatgc
     9121 cagcaactga atattcatag ttgtctgact taacagtttt accatcagca tccaaatagg
     9181 tgtcggtgaa gtaaaaacct tcataatcat catcaatcgt tgaattgctg atgttcagag
     9241 tgaaggtgtc accgtcaatc cagtcgtgag cttttgcagc gtcgccgaag ttaggaacat
     9301 attttgacgt gatcataccg tgaacagttg agttattaat actcacagta tttgcagtac
     9361 ctgctgcata gtcatcatag ttcaggtaat aggtagaaat tacaccattt acagtgtcac
     9421 ctgagaaaac ggtgtcatag taaccgctat ctttagctac tgtgttataa acataaccac
     9481 tgtaggtcag gtaatcggta tcaatgttgt aaattggttt gttggtatca acataaccaa
     9541 aagtattata agtagcagca ttagcctgag aggtgatagc cagagtgcaa gccaatgcta
     9601 attgtgatac tacaagtttc tttttccagg agtgcatttg tcatccctcc tcagggacgt
     9661 aataagttga tccatcaatt atcaatgcat agaaaacatc ttatgtttcg cgtttacgat
     9721 tatgcggcgt tatagaggaa tggttcaatt attctttccc ctaagtccga ttgcggacat
     9781 atataaaaat atctatacgc acaaagaata agttgacaaa aacactgtat ttcaacacgt
     9841 taatgaaatc attaattttg aattatagat cttgaaaata ccaa
//
LOCUS       DAZFKF010000147         9867 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFKF010000147 DAZFKF010000000
VERSION     DAZFKF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.149"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            424..1254
                     /gene="efeU"
                     /locus_tag="ACHKA1_004693"
     CDS             424..1254
                     /gene="efeU"
                     /locus_tag="ACHKA1_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO5682244.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            1312..2439
                     /gene="efeO"
                     /locus_tag="ACHKA1_004694"
     CDS             1312..2439
                     /gene="efeO"
                     /locus_tag="ACHKA1_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO5682245.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTEADRNALKGPITALAEDLAQLRGVLGLD"
     gene            2445..3311
                     /locus_tag="ACHKA1_004695"
                     /pseudo
     CDS             2445..3311
                     /locus_tag="ACHKA1_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
     gene            complement(3670..4281)
                     /locus_tag="ACHKA1_004696"
     CDS             complement(3670..4281)
                     /locus_tag="ACHKA1_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5682246.1"
                     /translation="MDKAEKTVSSAERFIDMEGINRFSERLAMAMGNMTNVELATLCG
                     LSEATVRNYRKGKNFPPLDKLALIAKACNCSLDWLATGKNEDECGKIGKNCDSGSGID
                     QALSSLSPQEKQLILTFIRREGTNNLVRLAAMNTSTISQDAVESIIDALPLRPVLKNA
                     IKIGMAGSEETDKEILRVIEKYNSSNNATQESGAVTVKKNVVG"
     gene            4438..4704
                     /locus_tag="ACHKA1_004697"
     CDS             4438..4704
                     /locus_tag="ACHKA1_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5682247.1"
                     /translation="MRVSRQASNNLFVREGCDWSAKAVYAALENKGTDLPELERELGL
                     KSGSMRNVFYRQCRKYEAAIAEKIGVEPAVIWPSRYQVDAREVA"
     gene            4715..6805
                     /locus_tag="ACHKA1_004698"
     CDS             4715..6805
                     /locus_tag="ACHKA1_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO5682248.1"
                     /translation="MWVTAKECVGLPDLPMMEHNIRHRLTKAAAGNAAMLRKRKGTKA
                     IEFNIDCLPSGAQQVLRERYALQLMTQKADESPAPVVTKARRSSAVVDAVEAYRGSPQ
                     LMLERLNALTENQRQVADARIAIVSEVLKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADVLDKEIVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFSALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPMKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRPDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVADDEPDEEYERNFQRGL
                     QLLEAKLKESDPLA"
     gene            6888..7820
                     /locus_tag="ACHKA1_004699"
     CDS             6888..7820
                     /locus_tag="ACHKA1_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5682249.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLTGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            7823..8044
                     /locus_tag="ACHKA1_004700"
     CDS             7823..8044
                     /locus_tag="ACHKA1_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682250.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATDVYSGICRSVGAKQVS"
     gene            8057..8311
                     /locus_tag="ACHKA1_004701"
     CDS             8057..8311
                     /locus_tag="ACHKA1_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682251.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            8313..8594
                     /locus_tag="ACHKA1_004702"
     CDS             8313..8594
                     /locus_tag="ACHKA1_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFO5682252.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRARG"
     gene            8591..8863
                     /locus_tag="ACHKA1_004703"
     CDS             8591..8863
                     /locus_tag="ACHKA1_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682253.1"
                     /translation="MSKVVRIIFEYKEDVIRKNADGTVHMRVSLDIRSTGIKQKNDSP
                     AMIFGVVMLAESRNFAELVAMKASAFMKDRGMASGVINGNEFNQQG"
     gene            8868..9161
                     /locus_tag="ACHKA1_004704"
     CDS             8868..9161
                     /locus_tag="ACHKA1_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682254.1"
                     /translation="MSKVRVIFEFEHVSHDEKPAGNDSVEVHEKIGVDVKTERDTDNR
                     PTSLCDVYASILQYHSPEIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            9173..9703
                     /locus_tag="ACHKA1_004705"
     CDS             9173..9703
                     /locus_tag="ACHKA1_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO5682255.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRRFGDMQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIHDWCKTNRNELTNGGETKTANLTTGTVSWR
                     LGTPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKQAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
BASE COUNT         2595 a         2283 c         2643 g         2346 t
ORIGIN      
        1 cactatcaaa aatcgctcat tccagactta aatacacgtt ttctcctgtt taggttgtgg
       61 gcgaaaaatt cgatgaggag acgaggtata atttcattca caaccctatc actatctatt
      121 aataagcaaa aaacaacgtt cttttgtgat acgactaact gaaaattcaa cccttcgggg
      181 cacgctgcag cgagtttgtc gccaccaaca ttcgtacaag cttcatctgc cgggcgaaac
      241 gtcagctaaa taccttaaaa taatcgcttt tttctccgga tttgatctac tcatttttac
      301 attccaacat cattttataa acattccgct tgtatttttc tttagcgtaa tgataatcgc
      361 tatcactgcg atttactttt ctttgcatag attgactcag aaaaacgttt aagggtgggt
      421 ggcatgtttg ttccatttct cattatgttg cgcgaagggc ttgaagccgc gctgattgtc
      481 agtttgattg ccagctatct taagcgtacc cagcgaggcc gatggattgg tgtgatgtgg
      541 attggcgtgt tgcttgccgc tgcgttgtgc ctgggcttgg gtatcttcat taacgaaacc
      601 accggcgaat ttccgcaaaa agagcaggaa ctgtttgaag gtatcgtggc ggtgatcgcc
      661 gtggtgatcc ttacctggat ggttttctgg atgcgcaaag tgtcgcgcaa cgtcaaagtg
      721 caactggaac aggcagtcga tagcgcattg cagcgtggaa atcatcatgg ctgggcgctg
      781 gtgatgatgg tcttttttgc cgttgcaagg gaaggcctgg agtcggtctt tttcctgctg
      841 gcggcatttc aacaagatgt cgggatctgg ccgccgctgg gtgcaatgct cggtcttgct
      901 actgccgtgg tgctcggctt cctgctctac tggggcggta ttcgcctcaa tcttggtgca
      961 ttttttaaat ggaccagcct gtttattctc ttcgtcgccg cagggctggc tgcaggtgcc
     1021 attcgcgcat ttcatgaagc cggattgtgg aaccactttc aggaaatcgc cttcgatatg
     1081 agcgcggtgc tctcaactca ctcgctgttt ggcacgttga tggaagggat ttttggttat
     1141 caggaagcgc cgagcgtcag cgaagtcgcc gtctggttta tttatctcat cccggcgctg
     1201 gtggcatttg ctctgccacc acgcgcaggg gcgacagcgt ctcgctccgc gtagcaaata
     1261 cgacgcaaac tcttgcttag ttacaacata ctttaaaggg atagtctcgt catgaccatt
     1321 aacttccgcc gtaacgcatt gcagttgagc gtggctgcgc tgttctcttc tgcttttatg
     1381 gctaacgccg ctgatattcc gcaagtcaaa gtgaccgtga cggataaaca gtgcgaaccg
     1441 atgaccatta cggttaacgc cgggaaaaca cagttcatta ttcagaacca cagccagaag
     1501 gcgctggagt gggagatcct caaaggcgtg atggtggtgg aagagcggga aaatatcgcc
     1561 cctggtttta gccagaaaat gacggcgaat ttacagcctg gcgaatacga tatgacctgc
     1621 ggtctgctga ctaacccgaa agggaagttg atcgtcaaag gtgaggcaac ggcggatgcg
     1681 gcgcaaagtg atgcgctgtt aagtcttggt ggtgcaatta ctgcatataa agcgtatgtc
     1741 atggcggaaa ccacacagct ggtgaccgac accaaagcct ttaccgacgc gattaaagca
     1801 ggcgatatcg aaaaagcgaa agcactgtat gcgccgacgc gccagcacta tgagcgcatt
     1861 gagccgattg ctgaactgtt ctccgatctg gatggcagca ttgacgcccg tgaagatgat
     1921 tacgagcaaa aagccgctga tccaaaattc accggtttcc accgtctgga aaaagcattg
     1981 tttggcgaca acaccaccaa aggaatggat cagtacgctg agcagcttta taccgatgtg
     2041 gtcgatttac aaaaacgcat cagtgaactg gctttcccac cttcaaaagt ggttggcggt
     2101 gcagccggac tgattgagga agtggcggcc agcaaaatca gcggtgaaga agatcgctac
     2161 agccacaccg atctgtggga tttccaggct aacgttgaag gctcgcagaa aattgtcgat
     2221 ctgctgcgtc cacaactgca aaaagccaac ccggaactgc tggcaaaagt cgatgccaac
     2281 tttaaaaagg tcgataccat tctggcgaaa taccgtacta aagacggttt tgaaacctac
     2341 gacaaattga ccgaggccga ccggaacgcg ctgaaagggc cgattactgc gctggcggaa
     2401 gatctggcgc aacttcgtgg tgtgctggga ctggattaag cgttatgcag tatgaagatg
     2461 aaaacggcgt gaatgaactg tcacgccgac gtttactgaa aggaattggt gcgctggcgc
     2521 tggcgggaag ttgtccggtc gctcatgcac aaaaaacgca aagtgcaccg ggcacgcttt
     2581 caccggatgc acgcaatgag aaacagccgt tttatggtga gcatcaggca gggatcctga
     2641 cgccacaaca ggccgcaatg atgctggtgg cgtttgatgt gcttgccagc gataaagccg
     2701 atcttgagcg gttgtttcgc ttgttgactc agcgttttgc ttttctgact cagggcggag
     2761 cagcaccaga aacgccaaat ccgcgcctgc caccactcga ttccggcatt cttggcggct
     2821 acattgcgcc cgataatctc accatcacgt tatcggtggg tcactcattg tttgatgagc
     2881 gcttcggcct tgcgccacag atgccgaaaa agttgcagaa gatgacgcgt ttccccaacg
     2941 actcgctgga tgcggcgtta tgccatggtg atgtgttgct acaaatttgc gccaacaccc
     3001 aggacacggt gatccatgcg ctgcgcgata tcatcaaaca cacgccggat ttgctcagtg
     3061 tgcgctggaa gcgggaaggg tttatttccg atcacgcggc gcgtagtaaa ggcaaagaga
     3121 cgccgattaa tttgctgggt ttcaaagacg gcactgccaa tcccgatagc cagaatgata
     3181 agttgatgca aaaagtggtg tgggtaacgg cagatcagca ggagcctgcg tggacaatcg
     3241 gtggcagcta tcaggcagta cgcttgattc agtttcgagt ggaattttgg gacagaacgc
     3301 cgctgaaaga acatgtaacc aggtgaatca tttgataccg acaagggttt tagtgaaagg
     3361 aggtgaaatc acctgtaaga tgctgaaata aaacaggtga tgtatacttc ggacggaaaa
     3421 ctctttttga taccgactgc tcaacaaata gtcaaaagat caaaaaacgg cgacacattt
     3481 gatactgcta atttaccctt aaaatcttct tcaaaacatc ttcaaactgc caaaatgctc
     3541 acataatcga tctaactata atcagtatga taatcatcat gaattactat tcaatagcgt
     3601 gattatcttg ggatgagtca tatgatctct atatgtattt acagcaccac ctcatgatag
     3661 cgatggcgct taaccaacaa catttttctt tactgtcact gcccctgatt cctgggtggc
     3721 gttattgctc gagttgtact tctcaataac gcgcaaaatc tctttgtctg tttcttcact
     3781 tcccgccatt cctatcttta ttgcattttt tagtacggga cgtaatggca aggcatcaat
     3841 gatgctttct accgcatcct ggctgattgt agatgtattc attgctgcca gcctgacaag
     3901 gttgttagta ccttctcgcc tgatgaatgt aaggattaat tgcttctctt gggggcttag
     3961 agagctaagc gcctgatcaa tacccgaacc tgaatcgcaa ttttttccga tttttccgca
     4021 ttcatcttcg ttcttgcctg tggccaacca gtcgagtgaa caattacagg ctttagctat
     4081 taaggccagt ttatccaaag gagggaagtt tttccccttc ctgtaatttc ttactgtggc
     4141 ttcagatagt ccacaaaggg tcgccagttc gacgtttgtc atgtttccca tagccatagc
     4201 taagcgttct gaaaatcggt ttattccttc catatcaata aaccgttccg cactggatac
     4261 ggttttttct gccttatcca tataaccccc tataaaacaa agaaattatc gacatcgcat
     4321 ttaatttcga tatgctcgca aaaaaccgtt gatatcgaat tattttgcga ttatgattta
     4381 cctaaatcga taaccggttt tgattatcgg ttcaggtaaa catgagagga tcacacaatg
     4441 agggtatcca gacaagcatc gaataatttg tttgttcgag aggggtgtga ctggtctgct
     4501 aaagcggtat atgcagccct tgaaaacaaa ggcacagacc tccctgaact ggaaagggag
     4561 ttaggattaa aaagcggatc aatgcgcaat gtgttttacc gccagtgccg taaatacgaa
     4621 gccgccatcg ccgaaaaaat aggggttgaa cctgctgtaa tctggcccag cagataccag
     4681 gtggacgcca gagaagtggc ttgatggggg atgtatgtgg gtaacagcaa aagaatgtgt
     4741 tggtcttcct gatttaccaa tgatggaaca taacattcgc catcgactca caaaagcagc
     4801 tgcgggaaat gcagccatgt tacgcaaacg taaggggaca aaagcaatcg aatttaatat
     4861 cgattgtttg ccttctggcg cgcaacaagt attgcgtgaa cgttatgccc ttcaactgat
     4921 gacgcaaaaa gcagatgaat caccggctcc ggtggtgaca aaggccagac gctcatctgc
     4981 cgtggttgat gcggtggagg catatcgcgg atcaccacaa ctgatgctcg aacgcctcaa
     5041 tgccctgact gaaaaccagc gccaggtggc tgatgcacga atcgcgatcg tcagcgaagt
     5101 tctgaaagtc gcgcaacaac ctggtttcag ctgtgcgaag gctatccggt ttatcgttga
     5161 caacctggca cgttcgcaac tggacgagcg cattgtggca atggttgaga cggcgaacgc
     5221 caaaaaggga aacagccgtg cgttgagtga aatcacgctg aaacgctgga ttgcggcctt
     5281 taacaaggca cagaacgccg ctgaacgcct gcttttactg gcaccgggta aacgccagga
     5341 aataaaagcc gaagatatta actggctgcc cgaatttctg gcgcagtatc gccagtcaaa
     5401 cggccgacca atgaccgagg cttacgagga ttttgttgct gaatggcagc accggcacgc
     5461 tgatgagcct tatatgctcg atatcatgcc ctcttatgac accattcgcc gcgcaatgaa
     5521 gaaactgccg gaagtggtga aacaaaaagg ccgggtaact ggcagtgaat accgtcagct
     5581 tgagggattc acgcgccgcg actggtccag aatgccggtg aattatgtct ggattggtga
     5641 cggtcacggc atgaagctga aatgcgcgca cccggttcac gggcggccat tcgcaccgga
     5701 agtgaccttt gttatcgacg gtggcacgcg ctttgtggtg ggctggagcc ttgacctggc
     5761 tgaaaatgtt ttcgccgtag ccggtgccat acagcacggt attcgccatc atggcaaacc
     5821 gtttctgtat tactcggata atggctccgg ggaaaccgcc gacgtcctgg ataaggagat
     5881 tgtggggata ctgccacgac tggggattaa tcacccgaca gggattgccg gtaatccgca
     5941 gggacggggc attatcgaac ggcttaaccg cacattaccg atgcgcatag cccgtaaata
     6001 ccgcacctat ttcgggaaag gagcagatcg cgagacgtta cgtaaaacca accgcgattt
     6061 acgctcggca ttcagtgccc tgcaacaggg caaacggctg aacgcccggc agcagtcagc
     6121 gatgcgtgat ttaccgtcct ggtctgaact gattgatgcc attcgtgacg gtgttgagtg
     6181 gtacaacaac cggccgcacg atgaattacc catgaagccg aacggtaaac attacagccc
     6241 ggcggagttc agaaaaaaac gcctggcgga agaggacacg gaaattgaat ggctgtccga
     6301 tgttgaactg cgggatatgt tccggccgat ggtggagcgc cctgtaagac gctgtgaaat
     6361 acgctggctg aataatattt actacgcgcc cgagctgcgt gacgagcatg gccgtaaggt
     6421 acttatcagc tatgacattc atgatgccga acgaattacc gtgcgccgcc cggatggcag
     6481 cgtgatttgc gaggcggtat gggacggcaa taaacgcgaa gccttccctg tcagcgcgga
     6541 atactacaaa cagcagcagc gccttaaagg catgcgtaaa cgcgcagagg aaaaaatccg
     6601 tgatgccgag gatgaggttg tcaacgtgct ggagcacaag ccgcaggagc cctggctgga
     6661 aaacatatac cgccctgtgg gtaatacggt gaccgttcag caaccggtcg ctgacgatga
     6721 acctgatgaa gaatacgagc gtaatttcca gcggggattg caactgctcg aagcgaaatt
     6781 aaaagaaagt gatccgctgg cctgaaataa aaaaataacc cgaggggtga ctcaggttat
     6841 ttgaggtttc agtctcggag aatgctgtct caagcgaggt gaataatatg accgatatta
     6901 acgatgtaat caagaccatt gatgaactta ttgatggcgg cgtactgacg cagtatgcca
     6961 tcgccagaga ggcgggaatt tccgacggca cattatcggc tttccgtaag ggaaaatata
     7021 aaggcgataa cgctgctgtg gctgcttccc tgcgctcctg gtatgagaac tggaataaac
     7081 aaagcgcact gccggaaccg ccgcagtttg tggaaactca gacagtccag gagctgcgcg
     7141 cactgtttca ggcggttcgc ctgatgggct gtattaacgt tattgtgggc gtaccgggtg
     7201 tgggtaaaac ggccactgcc cgtaattact gccaggagca accaaacacc tggatgatca
     7261 ccctgtcacc cgcgcactcc agcgtcacgg agtgtctgct ggagctggcc gatgcgctgg
     7321 ggattgatta cacccgcgcg aacaaagggg cattatcccg cgccatccgc cgtcgcctga
     7381 cgggaacgcg tggactggtg attgtggatg aggcggatca tcttggtatt gacggtctgg
     7441 agcaactccg ggcaattcag gacgccacgg ggatcgggat ggtgcttatt ggtaacccgc
     7501 gcggattgtt taaaggtgga cgccgcgcct ttgatgattt atcgcgcctg ttcagccgtc
     7561 ttgcccgtac aaaacaactt cgcaaggcca aaaaggcgga tgtgctggcc attgccaggg
     7621 catggggaat cagtggtgag gccgagctgg ccgtcatgca ggctatcgct gaaaagccgg
     7681 gagcgttacg cgttctgaca catacgctta accaggcgtg gctcaccgcc agcggtgaag
     7741 gcgcggcgct gacagaaaaa catattaatg cggcctttaa agaggtttat accaaccctg
     7801 aattactctc acaggtgtga ttatggcggt atttaatatt cctgatattt acggacgctt
     7861 ttacctggtt aatttcgata acgtgaaggt gatttcactg gccgaaaata aagaatgtgg
     7921 cgatttactt tttgaattta atgaccgcac acgaatggtg atatctgccg gacttgatcg
     7981 cgaaggtgcg acagacgttt acagcggaat atgccgttct gttggtgcga aacaagtcag
     8041 ctaaatgagg tgttatatga atatgcaatc ctgcggtaac aaaatgaatt tattcgactc
     8101 cctgaacagc gcgcgccgtc tgaccgaact tgccggtgcg gtactggaac gcagtaagcg
     8161 ctatccgcaa cgttttgcac tgaaaaccac gccgccggta ggcaacgtgc agggaaccgg
     8221 tgaaattgaa atcaccatac agaccaacgg cctgcgccgc cgtgtgaagg ccacccgcat
     8281 cagcggctgc acggtttact gggaggtgtg aggtgaaaaa aaatctcatt gcatgggcgt
     8341 ggtcgagtgg tcttattgag tttggttacg tcctgccgga aggtgcattg ccgatagttg
     8401 ccggaaagcc tgccacggta cggcatgtga ttgaggttat ggcgcgtcat ggacgtgatg
     8461 aacaggagca gttactggtt ccggggatac cggaagcggt gacggaggaa gaagccttta
     8521 atgccatgat tcggttctgc cgtgaggtca gacgccgggt cagttatcca aacagaacga
     8581 gggccagagg atgagtaaag tcgtgcgcat tattttcgaa tacaaggagg acgttatccg
     8641 taaaaacgct gatggaacag tgcacatgag ggtaagtctg gatatacgtt caaccggaat
     8701 aaagcagaaa aatgatagcc ccgccatgat ttttggggtg gttatgctgg cagaaagcag
     8761 aaacttcgca gagcttgtgg caatgaaagc cagtgcattc atgaaagaca ggggcatggc
     8821 ttccggggtt attaacggta atgaatttaa tcagcagggg taattccatg agcaaagtac
     8881 gcgttatttt tgaatttgag catgtttcgc atgacgaaaa accggcaggc aatgacagtg
     8941 ttgaagtgca tgaaaagatt ggtgtggatg taaaaacaga acgtgatacg gataacaggc
     9001 cgacgtcact ctgtgacgtt tatgcaagta ttctccagta tcacagtcct gaaattattc
     9061 agtttctctc agcggaattt caggcatctg tacaggcttt tggggcggat gccatcatta
     9121 aacgccaccg cgtgcataaa gcatcaggca cactgcaata aggaaaaaca aaatggctaa
     9181 acgcgttaca aaattaaagg ccgcagccga ggcagcaccg cagacccgtg aagaggtcag
     9241 ccgcgatatc cgacgctttg gggatatgca gcgtgaggca ctacgcctgg aaacggcgat
     9301 gaatgacgaa gtggcagaaa tcaccgcccg ttatacgccg cagattgaaa accttaaaaa
     9361 agaaatcaaa gtgcttttta aggggattca tgactggtgt aaaaccaacc gcaatgagct
     9421 gacgaacggc ggcgaaacca aaacagccaa tctgaccacc ggaacggtgt catggcggct
     9481 gggaacgcca tcatgcagcg tcagccgtga tgtggaaggc gtgattgaaa tgctgcgccg
     9541 tatgggcctt gagcgcttca tccgcacgaa agaggaagtg aataagcaag ctgtcctggc
     9601 ggagccagat gcggtgaagg gcattgccgg tataaaggtg aataaaggcg ctgaaagttt
     9661 ttatgtcgag ccttttgaac aggacgccgg actgaataaa taacaccgca ttaaatcttt
     9721 aaatatcaca tcgtgttaat tatggcgctc gcgtcagggg actgcctgcg cctgtaaact
     9781 gaaaataagg aacaggaaat atggcatatt tttatttcaa attagacata gtgtacacaa
     9841 ataaatattt aaccgaatat cagcggg
//
LOCUS       DAZFKF010000148         9739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFKF010000148 DAZFKF010000000
VERSION     DAZFKF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.150"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            352..1071
                     /gene="ykgE"
                     /locus_tag="ACHKA1_004706"
     CDS             352..1071
                     /gene="ykgE"
                     /locus_tag="ACHKA1_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO5682256.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPMYLADEPEWASRAA
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            1082..2509
                     /gene="ykgF"
                     /locus_tag="ACHKA1_004707"
     CDS             1082..2509
                     /gene="ykgF"
                     /locus_tag="ACHKA1_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO5682257.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLIAMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            2502..3197
                     /gene="ykgG"
                     /locus_tag="ACHKA1_004708"
     CDS             2502..3197
                     /gene="ykgG"
                     /locus_tag="ACHKA1_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO5682258.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(3440..4108)
                     /gene="ykgH"
                     /locus_tag="ACHKA1_004709"
     CDS             complement(3440..4108)
                     /gene="ykgH"
                     /locus_tag="ACHKA1_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO5682259.1"
                     /translation="MSEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYINK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIINWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLILGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(4290..6587)
                     /gene="hyxB"
                     /locus_tag="ACHKA1_004710"
     CDS             complement(4290..6587)
                     /gene="hyxB"
                     /locus_tag="ACHKA1_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter HyxB"
                     /protein_id="HFO5682260.1"
                     /translation="MKNSKAFYRSALATAIVMALSAPAFAADNAVSTTPVTLDKDKTT
                     LDQDVVINGDKKITAVTIETSDSDKDLNVTFGGHDITAASTVNQDFVEGVKVSGNKNV
                     VINATDSTITAQGEGTYVRTAMVIDSTGDVVVNGGNFVAKNEKGSATGISLEGAKGNN
                     VTLNGTTINAQGNKSSSNASTAIFAQKGSLLNSFEGDATDNITLAGSNIINGRIETIV
                     TAGNKTGIHTVNLNIKDGSVIGAANNKQTIYASASAQGTGSATQNLNLSVADSTIYSD
                     VLALSESENSAATTTNVNMNVARSYWEGNAYTFNSGDKAGSNLDINLSDSSVWKGKVS
                     GAGNASVSLQNESVWNVTGSSTVDALAVKDSTVNITKATVNTGTFASQNGTLIVDASS
                     ENTLDISGKASGDLRVYSAGSLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQG
                     ADGNFYFVKNTHKASNASSVIQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWI
                     QYFGGKQKHTTAGNASYDLDVNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSK
                     GDTEGYSFHAYLSRQYNNGIFIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAM
                     VKGGYTWEDGNGLFIQPYAKLSALTLEGVDYQLNGVNVHSDSYNSVLGEAGTRVGYDF
                     AVGNATVKPYLNLAALNEFSDGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAY
                     ASLDYTKGDDIENPLQGVVGINVTW"
     gene            complement(6629..7390)
                     /gene="hyxA"
                     /locus_tag="ACHKA1_004711"
     CDS             complement(6629..7390)
                     /gene="hyxA"
                     /locus_tag="ACHKA1_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFO5682261.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVCNITYIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAENLPVMDSYSVFMNEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            complement(7544..8338)
                     /gene="hyxR"
                     /locus_tag="ACHKA1_004712"
     CDS             complement(7544..8338)
                     /gene="hyxR"
                     /locus_tag="ACHKA1_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HFO5682262.1"
                     /translation="MKKPLVIISACQFTRLALESLIPADRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHAKYVGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNMSKKRPGLL"
     gene            complement(8668..9231)
                     /gene="ipbA"
                     /locus_tag="ACHKA1_004713"
                     /gene_synonym="fimX"
     CDS             complement(8668..9231)
                     /gene="ipbA"
                     /locus_tag="ACHKA1_004713"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HFO5682263.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
     gene            9476..9610
                     /locus_tag="ACHKA1_004714"
     CDS             9476..9610
                     /locus_tag="ACHKA1_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682264.1"
                     /translation="MCVTSNLLVMFSSFEIIDVISYEVVFIIYLIDFICILSFHEVRV
                     "
BASE COUNT         2603 a         2140 c         2252 g         2744 t
ORIGIN      
        1 tattacttga tacgtgatta ttgtcggtga tttttttgta caccatcagt gtatatctca
       61 ataacccctg aataagtagc tctgaatagg tataagggat gtagccattt ttaaatggat
      121 ttcttatgcc aaaaatgatc gtcgatttcc ccataaaatg tgagcgatgc cgaaagaaat
      181 aaaattagtt attgcatttg aagtttggat gaaagattat catttgtcat acaaatgagg
      241 ggtggtatgt tgctagccaa ttaaaaaaga acgccatatt tattgatgat tgatcccccg
      301 gtggggggaa acatcgggca taaatgggca tgaagtaatg gagtattagt tgtgaatgtc
      361 aatttctttg tcacctgtat tggtgacgcc ctgaaatcaa gaatggcacg agactccgtg
      421 ctgctactgg aaaaactcgg ctgtcgcgta aatttcccgg agaaacaggg atgctgcggt
      481 cagcctgcga tcaatagcgg ttatatcaaa gaagcgattc cagggatgaa aaatctgatc
      541 gccgcactgg aggataacga tgatcccatt atttcaccgg ctggctcctg cacctatgcc
      601 gtaaaaagtt acccgatgta tctggcggat gaacctgaat gggcatcacg tgccgcaaag
      661 gttgccgcgc gtatgcagga tctcacctct tttattgtta ataaattagg ggtagtcgat
      721 gtaggtgcca gtttgcaagg gagagcggtg tatcacccat cttgtagcct ggcccgtaag
      781 ctgggagtga aggacgagcc acttacgctg ctgaaaaatg tgcgtggact ggagctgttg
      841 acctttgctg aacaggatac ctgctgcgga tttggcggca cgttctcggt caaaatggcc
      901 gaaatatccg gcgagatggt gaaagaaaag gttgcgcacc tgatggaagt ccgccctgag
      961 tatttaattg gtgctgacgt gagttgcctg ctaaacatca gtgggcgatt acaacgggaa
     1021 gggcagaaag tcaaagtgat gcatattgct gaagtgttga tgagccgctg aggatataaa
     1081 gatgtcgatc aaaaccagta atacagattt taagacacgc atccgtcagc aaattgaaga
     1141 tccgatcatg cgcaaagcgg tggcaaacgc gcagcagcgt attggggcaa atcggcaaaa
     1201 aatggtcgat gaattggggc actgggagga gtggcgcgat cgggccgccc agatacgtga
     1261 tcatgttctg agtaatctcg acgcttatct gtaccagctc tcagaaaaag tgacgcaaaa
     1321 cggcggtcac gtctattttg caaaaaccaa agaagacgct acccgctaca ttttacaggt
     1381 tgcccaacgc aaaaatgccc ggaaggtggt gaaatctaaa tcgatggtga ccgaagagat
     1441 tggtgtcaat catgtgttgc aggatgctgg cattcaggtg attgaaaccg atctgggtga
     1501 atatattctc cagctggatc aagatccgcc atctcatgtt gtggtcccgg caattcataa
     1561 agatcgccat cagatccgtc gagtgctcca cgaacgtctg ggctatgagg ggccggaaac
     1621 gcctgaagcg atgaccttat tcatccggca aaaaatccgc gaagatttcc tcagtgctga
     1681 aataggtatt accggctgta atttcgcggt ggcagagaca ggttcggtat gcctggtgac
     1741 caatgaaggt aatgcgcgaa tgtgtaccac gctgcctaaa acgcatattg cagtgatggg
     1801 aatggagcgt attgccccca cgtttgccga ggtagatgta ttgatcgcca tgctggcgcg
     1861 cagtgccgtt ggtgcacgtt tgacgggata caacacctgg ctgacaggac cgcgcgaagc
     1921 ggggcacgtt gatggtcctg aagagtttca tctggttatt gtcgataacg ggcgttctga
     1981 ggtgctggcc tctgaatttc gggatgtgct gcgctgtatt cgctgcgggg cttgtatgaa
     2041 tacttgtccg gcatatcgcc atattggcgg tcatggatat ggctctattt atccagggcc
     2101 aattggtgcg gtgatttctc cgctacttgg cggctataaa gattttaaag atttacccta
     2161 cgcctgctct ttatgcactg cttgtgacag cgtgtgtccg gtgcgtattc cgctgtcaaa
     2221 actgattttg cgtcatcgtc gggtgatggc tgaaaaaggg atcaccgcaa aagcagagca
     2281 acgggcgata aaaatgttcg cttatgccaa tagtcatcca ggattgtgga aagtcgggat
     2341 gatggccggc gctcatgcgg caagctggtt tatcaatggc ggcaaaacac cactcaaatt
     2401 tggcgcgatt agcgactgga tggaagcacg cgatcttcct gaagctgacg gagagagttt
     2461 ccgtagttgg tttaagaaac atcaggcgca ggagaaaaag aatggataat cgaggcgaat
     2521 ttttgaataa cgttgctcag gcactgggtc gcccgttgcg acttgaaccg caagcagaag
     2581 atgcgccgct taacaactat gctaacgagc ggcttaccca acttaaccaa cagcagcgct
     2641 gtgacgcgtt tattcagttt gccagcgatg ttatgttgac gcgctgtgag ctgaccagcg
     2701 aggcgaaggc ggcagaagct gcaatacgtc tgtgtaaaga gctgggagat cagtcggtcg
     2761 tgattagcgg tgacacgagg ctggaggaat tggggattag cgaacgtttg cagcaggaat
     2821 gcaatgccgt tgtttgggat ccggcgaaag gtgccgagaa tatctcgcag gcagagcagg
     2881 ctaaagtggg tgtcgtgtat gctgaatatg gtttaaccga atcgggaggc gtggttcttt
     2941 tttccgccgc cgagcgcggg cgttcattga gcctgctccc ggaatcttct ctttttatcc
     3001 tgcgtaaaag cacaatactg ccgcgtgtag cgcaactcgc agaaaaattg catcagaaag
     3061 cgcaggccgg tgaacgcatg ccgtcctgca ttaacatcat tagcggccct agttcaacgg
     3121 cggatattga gcttatcaaa gtcgtcgggg ttcatggccc ggtgaaagcg gtgtatctga
     3181 ttattgagga ttgctgagag agatatgaag tctggacata aacctgaagg cggcatacga
     3241 ggttgtgaat agcgttaagg acaacctgac tgaaccccgt tccgtcatgg ttatgctcct
     3301 cttctgtaaa tatcctggca ttagtaatgc caggatattt acagtgaaaa agacaggcat
     3361 ccattcctga aaacgggttc ccttcccggt cattgtcact tcagctttat acaggcactc
     3421 tctatcagaa tgctgtttat tactgcccag agttttgttg gtattcatct accatttttt
     3481 tgacaaggca aaacattaca gaaatcacaa tgcttagaaa aaatcccaga atcaggcaaa
     3541 tggcagcagg gtatttattt tttatttctg gtgagcttat agttacacag ccatcaccgt
     3601 tcggatacaa tgaccagttc ctggtcaggt atatcatttt tgtataaagc tcactgttaa
     3661 ttgctgaagg tgtttgtatc tcatcgagaa cttgcctgcc attatggtat tctgacgcat
     3721 accaattgat aatagacgcc tgcacgcgag atatttccgc ttcagcagtt ggtaaatctc
     3781 tcttatctcc tgaccattcc agctttattt ctctgttctt gacatctggg ctggtgtgaa
     3841 aaaatgtagt aattcgctga ataacatctt cagtcattgt ggtctgacag gcaaagttat
     3901 tattgctatc acagttaaca agtatgcctg gtgttgtctg atttattttt aggctgtaac
     3961 tcacttttat attatcttta ttgatataca gaaacaacag caccattgcc atacagatag
     4021 ctataataaa aaggataaca cgaatctttt tcttcagata aaataaaatc tcaattagat
     4081 cgatatcctg tttgatttgt tcactcataa tatatccaga aaataaaatc tgccgcagat
     4141 aaatgaatta atagtctgta tcaggaatgt tcgggttaaa tatcagcaaa aagccagcat
     4201 catgaatact ggatatttat agcctgcagg cagcatactg ctgcctgcag ggggtataac
     4261 agtcgacagg ggaaccgact gctttattct taccaggtca cattgatacc aactacaccc
     4321 tgtagcggat tctcaatgtc gtcacctttg gtgtagtcaa ggcttgcata tgctcccatg
     4381 tttttggtga tatcagcctg tacacctgca cccacgcgga atgctgcacc gtcaatgctg
     4441 gcattgacag actcatcacc cagacggact ttgttgccat cagagaattc gttcagtgcg
     4501 gccagattca gataaggttt aacggtcgcg ttgcccacag cgaagtcata acccacgcgc
     4561 gtaccggcct cacccagcac agagttatag ctgtcagaat gaacgttcac gccgttgagt
     4621 tgataatcca caccttccag cgtcagcgca gacagtttgg catatggctg aataaacagg
     4681 ccattaccgt cttcccaggt gtaaccgcct ttaaccatcg caccgaaacc attggtgtta
     4741 aagtcagctt tgatggtacc gccaccattc atcaggcgaa catctgccgt gttgctgtag
     4801 tgaccaaact gtgcagcagt atcaatgaag ataccgttgt tatactggcg gctcaggtaa
     4861 gcgtggaagc tgtaaccttc agtgtcgcct ttgctctgca tggtggtcat gtcacctttc
     4921 gcagaagaca tcgccacacc ggccagccag ctaccatctt cagtcatgaa gcgggtatca
     4981 ccacccagca ttacaccgtt tacatccagg tcataggatg catttcccgc ggtggtatgt
     5041 ttctgtttac cgccaaagta ctgaatccat acgccgccct tgtcattttc gctcagacgg
     5101 acagcatcct gacgggctga gagcgtgtca gcctgcagat tagcaacgtt agccggagct
     5161 gctgccattg cctgaatcac ggagctggcg ttggatgctt tatgcgtgtt ttttacgaaa
     5221 taaaagttac catcagctcc ctgtgtcagg tcatattgat acagaccacc ttccgttgtg
     5281 cctgtggctt ttagagtgct gtctttgccg gtagaaataa atgccgtttg ttcattgata
     5341 agatccaatg aacccgcact gtaaacacgc aagtcaccgc tcgctttacc gctgatatcc
     5401 agagtgtttt cagaagaggc atcaacaatc agagtgccgt tttgagaagc aaacgtgcca
     5461 gtattgactg tagccttcgt gatattaacc gtactgtctt ttactgccag agcatcaaca
     5521 gttgaggagc ccgtaacatt ccagacagac tcgttttgca gagatacact ggcatttcct
     5581 gcccctgaaa ctttgccttt ccagactgaa ctatcggaaa gatttatatc cagattacta
     5641 cccgctttat cgccgctatt gaaagtataa gcattacctt cccagtaaga gcgggcaacg
     5701 ttcatgttta catttgttgt ggtagcggct gaattctcgc tttcagaaag ggccaggaca
     5761 tcagagtaga tggttgaatc agcaacagac aaatttaaat tttgcgttgc tgaacctgtg
     5821 ccttgtgccg aagcagaggc ataaattgtt tgtttattat tagccgcccc aattactgag
     5881 ccatccttaa tattcaggtt gactgtatga attcccgtct tattcccggc agtaactatt
     5941 gtttcaatcc gaccattaat aatatttgag ccagcaaggg taatgttgtc ggttgcatca
     6001 ccttcaaaac tattcaataa gctacccttt tgagcaaaaa ttgccgtgga ggcgttgctg
     6061 gaactcttat taccttgagc atttatggtt gtgccattaa gcgtgacatt atttcccttg
     6121 gccccttcca gagatatccc tgtcgcacta cctttttcat tttttgcaac gaaattaccg
     6181 ccattaacaa caacatcgcc agttgaatca atgaccattg cagtccggac ataggtgcct
     6241 tcaccttgag ctgtgatggt ggagtctgta gcattaatca caacattttt gttaccacta
     6301 acttttacac cttcaacgaa atcttggttt accgttgatg cggcggtaat atcatgacca
     6361 ccaaaagtaa cattaaggtc tttatctgaa tctgacgttt caattgttac ggctgtaatc
     6421 tttttatcac cgttaataac aacatcttga tccagagttg tcttatcttt atccaatgta
     6481 accggtgtag ttgataccgc gttatcagca gcgaatgctg gtgcagaaag agccataaca
     6541 atagctgtcg ctaatgcgct gcggtaaaat gccttactgt ttttcatgaa gaaattctcc
     6601 attctggtaa ctaatttcaa tttcccactt atttatattt atgcagagat aaagcctgcg
     6661 tccctgcatt taccaccacg gaatacctgt tggtatttcc gttttcacgc aatgtcatgg
     6721 cattcgtttt ttctaatgca tttccatcac cgtgaaattt attgctgctc agcagcaggg
     6781 cttcccgcaa tgacatgctg tccggtattc cccaacgctt ttttagcgac tgctcggcaa
     6841 gccatatctg tcttatcccc gttttgagaa actgcgcact gtattcccaa ctatgtccgg
     6901 caagaagcaa cgacaacact ttcttttgct tttccgggaa gcgttctgac atgacgattg
     6961 tctttgtgcg gttattcacc tcattcataa agacagagta actgtccata actggcaaat
     7021 tttctgcatg tttcagtcct gaaaacccac cagaggcatt tgcaatactg atgacacgct
     7081 ctactgacat tcctggtttc agtaacctca ggtttttgag aacgccagaa atactcacat
     7141 ccggtggcag aaaaaagggc gcgacgcaca acaccaccac gaatgagtga tcccgataaa
     7201 atcggaacca catttcagcg cattcggcag gtcgccctgc cgatatcacg acactctgtg
     7261 gcctgtgccg caatttattg cgtaactcct cttctgttct gatgtaggta atattgcaga
     7321 cagaaccggt tgatagagct gcgaactgtg caggtacaat tcctgaggga cagaaaagaa
     7381 cgaaacgcat ccttccctca cagatgtcag tacgccagca cgctgtacag gctgtacagc
     7441 gtgcctccca atcctgcagc ccagagcagt agaaatattt tggcccctac actgtagcga
     7501 gtccgtacgc gccgcagtgg ggggtagtaa caacgctgct gtgtcataac aatcctggtc
     7561 tttttttaga catatttttt ctttgtccgg aaagtacttt tccggatatg gcgtcagaat
     7621 ttattgattc accaacggca actttctctg gataaagaat tccggatacc atgttctgca
     7681 catactgagt gagtcgatga gcggaatgtt gtccgacata ttttgcatgt agccgtatat
     7741 tccgtttcac tctgctgtca ccgcatcccg tatagagcag cctgaaatcg ctcagagttc
     7801 ccagtccaag tttcattaat gcccgtgtgg caatcgcatg cagggtcttt gaactgcgtt
     7861 gttcataacg ggcaatctca ctgaaactca tgccctgcat cagaatctta agcacgctca
     7921 actcccgtgc agtcaggagc cagaaatcgc tcatatcgcc gggcaaagga cgtagtaacc
     7981 agcggataat ttgattctga taacgactcg ctgtcagcat ggtgccgatg cgtgggaagc
     8041 cgttatacag acttgcagca ggagcattat cgaaccagtc ggagtcaccg gtcaggcaga
     8101 cctgccattg cccggcatca ctccgcaagc ggacaagaca agccagaaga actaactccc
     8161 ccgaaggaag cgaagggata tcagccaaca gatagccaca ggaggtcttt aaaacgaatt
     8221 caacctctgt agtgacgttg ctgtacaccc gtacgatata cctgtccgct gggatgagag
     8281 actccagtgc cagcctggtg aactgacagg cactgataat aaccagaggc ttcttcattc
     8341 agactcgtcc acgccattgc tacaaaacct gacattttga ttcaaaatgt caggtttggt
     8401 ttaattgcct gaatatttaa ttttcttgga atataaatct aatttgtttg atttttattg
     8461 tttttaattt tgtgttattt aacaatatga gcgaaagtta atcactggta tgacctttgt
     8521 ggttaatcat ttcaaatcat ggggttattt tactttagcg agaaatatca acatgttatg
     8581 tgttttattt aattgaatgg gttgtttttt tatggaagaa ttgaggaaaa cgtaggaatc
     8641 tgacattttg aatcagaagg tactgtttta acgaggcttt tttttccaca cccctttaaa
     8701 tcgagcagca ttactggctg tgtatctaac agtatgctgg atatttcgat gacccagata
     8761 atcctgaagt agtcgggtat cgacaccatt gtcagccaga gcaaaaccac aggcatgacg
     8821 taacatatga ggatgtgctt ttacagctaa tcctgcacgt tttccagcct cacgaatgat
     8881 agaaaaaaaa tgttgtctgc tgatagggcg gcgtttccgg gagagaaaaa gccagtctcc
     8941 ttcaactccg ttttcaatta acttcctctg cttaagccac aacttgatca aattatattc
     9001 atcaggaaga agaggatgtg tcgtcgagaa accattttta atacgccgaa tattaagttg
     9061 ttttcctgat gcatcaatat cggataaacg taaatcaaga agttcactgg ccctaaaacc
     9121 gtgaatgaaa gccatcataa ttagacagcg atttctttca ggaaaggaca tgctctgggc
     9181 tgcatccatc agcctgtaga cttcatcctg tgtgagatat tttctcgtca tacgccaccg
     9241 tgtacagttg taaaaaagag aggcacaccc agataagtat gcctgccatc agaacgttat
     9301 cgatgtaatc tcattatctc attaataaga ttttttgaat agtagttcta cgcaatagac
     9361 taaatatgaa ttatttattg tgcagctttt ctacaagcca tttttaatgc gtgtttttat
     9421 ctacagcgat gtggaaaaaa taaatataaa agctatgaat tacagggcgt gggggatgtg
     9481 tgttacgtct aatcttttag tgatgtttag tagttttgag ataattgacg ttatttctta
     9541 tgaggttgtt tttattattt atcttattga ttttatatgc attttgtcgt tccatgaagt
     9601 tagagtgtag aaaataaaaa tttaatatta ttcccgttaa cttacaataa cacattgttg
     9661 taagatattt tttagtcgta agtaaatcat tattgatggg ttctgctaat aaattaaaga
     9721 tgttctctta aatagtttt
//
LOCUS       DAZFKF010000149         9728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFKF010000149 DAZFKF010000000
VERSION     DAZFKF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.151"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..75
                     /locus_tag="ACHKA1_004715"
     CDS             <1..75
                     /locus_tag="ACHKA1_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682265.1"
                     /translation="SGEMLAVNPEQIDALIEFLKEIRD"
     gene            327..533
                     /locus_tag="ACHKA1_004716"
     CDS             327..533
                     /locus_tag="ACHKA1_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682266.1"
                     /translation="MAEEKKGGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            533..1588
                     /locus_tag="ACHKA1_004717"
     CDS             533..1588
                     /locus_tag="ACHKA1_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5682267.1"
                     /translation="MAVKAIALNTHQLFAYLNREDIAEFKFSPLFTALFFPNVATFNT
                     QDIMLDNLDIEEVTMSAFCSPMVGSQVQRDKGYETSIIRPGYMKPKHEIDPLKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPEKNIIEQADGKKWSEQDKDIHDPIYDIELYADQAGCPANVMIMGVDVWRMLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDLALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMEQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            1600..1935
                     /locus_tag="ACHKA1_004718"
     CDS             1600..1935
                     /locus_tag="ACHKA1_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO5682268.1"
                     /translation="MNTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGQESGKAVYLTSYPVDTSTQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            1948..2361
                     /locus_tag="ACHKA1_004719"
     CDS             1948..2361
                     /locus_tag="ACHKA1_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFO5682269.1"
                     /translation="MATNESIMTLPLASKFKAEARAMADRGLSTYEAVYQLNKLEEQD
                     KPRSDAIMALDESGDYQPLLRAMANVPCIDVGTAKSILSMTIEQERQKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRKPGDDRDIDRILKTI"
     gene            2567..3109
                     /locus_tag="ACHKA1_004720"
     CDS             2567..3109
                     /locus_tag="ACHKA1_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5682270.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IDNRVNNAISQLTNDKGEIDDDELLRVRIRLLTVQAEAQELKNERDRGNVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
     gene            3365..3646
                     /locus_tag="ACHKA1_004721"
     CDS             3365..3646
                     /locus_tag="ACHKA1_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682271.1"
                     /translation="MTEAELLGLIRRVAGISQQADEQTAQPDSVTAENYARVVAEVMR
                     RDGIQLNDVDMRNIRIRVLEMLAYRRRVQTYREKAKITYHWKKPERLRR"
     gene            complement(4460..7051)
                     /gene="yejO"
                     /locus_tag="ACHKA1_004722"
     CDS             complement(4460..7051)
                     /gene="yejO"
                     /locus_tag="ACHKA1_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFO5682272.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGAIKATTRVMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSVWTIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDIANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            7371..8018
                     /gene="narP"
                     /locus_tag="ACHKA1_004723"
     CDS             7371..8018
                     /gene="narP"
                     /locus_tag="ACHKA1_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO5682273.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(8053..9105)
                     /gene="ccmH"
                     /locus_tag="ACHKA1_004724"
     CDS             complement(8053..9105)
                     /gene="ccmH"
                     /locus_tag="ACHKA1_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO5682274.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVLEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(9102..9659)
                     /gene="dsbE"
                     /locus_tag="ACHKA1_004725"
     CDS             complement(9102..9659)
                     /gene="dsbE"
                     /locus_tag="ACHKA1_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO5682275.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
BASE COUNT         2593 a         2453 c         2295 g         2387 t
ORIGIN      
        1 tccggtgaaa tgctggcggt aaatcctgaa cagatagatg cattgataga atttctgaaa
       61 gagatcagag actgagccag cacacagcac acatagccgg agcaatccgg cttttttgcg
      121 cccaaaaaaa gcccgataag gtcagagggt tcttatcggg cttttgcata tgaggttttt
      181 ggatgcactg gcgttcgtga tcgggataat catttcataa tttgcaacat aactcaatat
      241 cattgcataa aatgcaattc tgattataat caggactgga taaacatcca gttataattt
      301 ttttagtcga agaggaattt cttactatgg ctgaagagaa aaaaggcggt gtttcggtgt
      361 acataagccc cgaaatcgtg gaggtgctca agcagcgcca caaaaaaaac tatgaagctg
      421 gcgttgcggc tggactggat ccgctgatga cgccggagcc gtcgataggt tcacttgtgc
      481 gatcttattt acttgcggcg cttgggatgc acaaaaatta tgggggcgaa taatggcagt
      541 caaagcaata gcacttaaca cacaccagct tttcgcgtac ctgaatcgcg aggatattgc
      601 ggaatttaaa tttagtccgc tgtttaccgc gctgtttttc ccgaacgtgg cgacattcaa
      661 cacgcaggac atcatgttag ataacctgga tattgaagaa gtcactatgt cggcgttttg
      721 ttcgcctatg gttggcagcc aggttcagcg cgataaaggg tacgaaacca gcattatccg
      781 ccctggctac atgaagccaa aacacgaaat cgatccatta aaaacaataa tgcgcatggc
      841 tggagaagat ccggcacagc ttaacgatcc tacctaccgc cgtatgcgcc tgattactgg
      901 caacatgcgc cgccagataa acgccattaa ggcacgcgtt gaatggctgg cggtgaatgc
      961 ggtaacgacc ggaaaaaaca tcattgaggg cgaaggcata gaacgctatg aaatcgactg
     1021 gaagataccg gaaaaaaaca tcatagagca ggccgacggt aaaaaatggt ctgagcagga
     1081 taaagacata cacgacccaa tatatgacat tgaactatac gcagatcagg caggttgccc
     1141 cgccaacgtc atgattatgg gcgttgatgt atggcgcatg ttacgcagct ttaaaaaatt
     1201 ccgtgaactg tacgatctct cccgtggttc agaatccgcc gcagagctgg catgtaaaaa
     1261 cctgggcgaa gtggtgagct ttaaaggcta tcttggtgat ctggccctta tcgtctattc
     1321 cggcaaatac actgacagcg acggcacaga aaaatatttc cttgagcctg atttgctggt
     1381 cctgggcaac accaacaata aagggctggt ggcctatggt gcgattatgg aacaggaagc
     1441 ggtaagaacg ggcgcaacgc aaaacatgtt ttacccgaaa aactggattg aagacggcga
     1501 tccggcgatt gagtacgtgc agacacacag cgcaccgcag cctgttccgg cagatattcg
     1561 aaaatttgtt accgtcaaaa ttggttaacg gtggattcta tgaacactcc atacattgag
     1621 ttatttgcag gcagtcagca ggtatcaacg acgctggtac attttgccgc tgatgctggc
     1681 gttattcagg aatttacccc gctgatgctg gcggacaatg gcgagtttaa gccgtgggat
     1741 ggtcaggaat ctggcaaggc tgtttatctg acctcgtacc ccgttgacac gtcgacgcag
     1801 aaatcagcac agtgctataa gacggggata tttaatatcg ccgccgttaa ctggcctgag
     1861 agcgtcgaca ccgatgcgaa aaaatgcgcc gcctttgcgg gttctggcgt atccgttcag
     1921 ccgctggcgc gataagcagg gggaacgatg gcaacgaatg aaagcatcat gacgctaccg
     1981 ctggcgagta aatttaaagc cgaagcgcgg gcaatggctg acagagggtt atcaacctac
     2041 gaggccgtat atcaactcaa caaactggaa gagcaggaca agccgcgctc tgatgcgatt
     2101 atggcgcttg atgaatctgg cgactatcag ccgctgttac gtgcaatggc aaacgtgcct
     2161 tgtatcgatg ttggtacggc taaaagcatc cttagcatga ccatagagca ggaacgccag
     2221 aaggttgcac cagagcttac cgcagccttt gaaaacttta tggacatgca cagcccgcaa
     2281 gccgtatcag caggcatggc atacgatggc agaaagccgg gcgatgaccg cgacatcgat
     2341 cgcatactga aaaccatctg agacaggccg gagaaatccg gcttttttta cgggtccttt
     2401 ccggcatatg gacccgttac ggggcggcga cctcgcggat tttcgctatt tatgagcctt
     2461 ttcgggttgg cggtggtggt tttgttgttt gctctatctt tatgaatgaa aagggaaaga
     2521 tgcaaacaat acaccaacct gaagcagtaa ttaagttggt gtattaatga aatcgcacct
     2581 gatgaacaaa aaaaacatgg cgaaaagctg ccgtgtaagt gcgacagcgt tcgacaagtg
     2641 gggagtgaca cccgttgaac gtaaaggccg tgaggcgttt tatgatgttg ccagcgtgat
     2701 agacaatagg gttaacaatg caattagcca gcttacaaac gacaaaggcg agattgatga
     2761 tgatgaactc ttacgagtca ggatcagatt actgacagta caggcggagg cgcaggaact
     2821 taaaaacgag cgcgatcgcg gtaacgtgat tgatactgag ttttgcctgt acgcgctttc
     2881 aaagctggcg agtcagattt catcaatcat ggacagcctc ccgcttacta tgcaaaggag
     2941 cttcccacag attacccccg ccatgctgga taggcttaaa agggaagtgg ttaaagcctg
     3001 taatgccagt gccagagttg ccgacaacct cccacagata ctggctgatt acttgaaaga
     3061 aacaaccgga aacgtaccgg aaaagttgct acagaagaaa ggcgagtaac agacgcgcaa
     3121 ttattgaaca aaaatgagaa aggatctgat aatggcattt tctggctaaa atgcctcgtg
     3181 taagatcctt tcttttttta atattttcat tgttaaacat tctgttacag agaagaagaa
     3241 cggatctgct ttttccctaa aaattttcat aaatagcgaa aacgcgcgag gtcgccgccc
     3301 cgtaacgggc cataattcca ggaaggaccc tacgacacca gactatcaga gcgatgtggg
     3361 cacaatgaca gaagccgaac tactgggatt aatccgccgc gtcgccggaa tcagccagca
     3421 ggctgacgaa cagaccgcgc agccggacag cgtgaccgcc gaaaattacg cgcgtgtggt
     3481 tgctgaggtg atgcgccgtg atggtatcca gcttaatgat gtggatatgc gcaacatacg
     3541 gatccgcgtt cttgaaatgc tggcctaccg tcgccgcgtg cagacgtaca gggaaaaagc
     3601 aaaaattacg taccactgga agaagccgga gcggttacgg cggtaactgg ttgatatttt
     3661 cgaaattcgc cagcggcgaa catcggggaa accagcctaa cccgttgata tttccggcaa
     3721 cctcaatttg aggagatcgg aaacatcacc cttcaaattc tgaatggcca taatgaccac
     3781 gcagaccatc aaggcaacca caacgaacat cgttacggtt ggagctttta ccccactggg
     3841 ggaaaggtat tacgataatc ataacaccta ccgaacaggt aaaacccacc agcctgatta
     3901 acagttaacc ggaaaaaatc cggcatgggg ggggagtggt tatccacgaa caacgaataa
     3961 gctggacacc gcgccccctc tcacgcagag aaaaaattac cgtttagtag tagttaacat
     4021 gttaactacc tgatgaagcg tttttattga ttttcccttt atgattcatc tggttgcgtg
     4081 caaaaaatgt taactacctt tttggtaaaa atgttaacag ggcagcggtt aacatttaac
     4141 ctggcaaaaa ttccggtagg tggggatccc catatcgggg aacatccacc agcttcgttt
     4201 ttataaatta aacacccggg aaaatccgac tctgaattgt gcattttttc agcaaatcat
     4261 tttagttata cgtttggtta tacggtttaa aagttgaatc aggaacattc ttttaaatca
     4321 ttcagttaca tgatcaatta aactactctc gtggaccaaa aatcccaaaa aaaaccaacc
     4381 cttacggttg gtttttttat atctgcaatt aattcgataa acagaccgtg acacatcaca
     4441 gcctgtttat tttctgttat cagaacgtcc agaccacacc cgcctgagta ttccacggcg
     4501 attctacccc tgcgccattg ccatagctga ctgacaaatg accgcttaac gtcggggtaa
     4561 acgatgacct tatcccagcc tgataaacgc cactggttcc agccacatca ttattaaatt
     4621 tgccatcgtc attaactttc acctggttag aatcggcgta ttcctgacgc acggcggctt
     4681 tcagccaggg ttccagcgtc gtaccgtttt gcaggtccat gtgatagctt accgccgttc
     4741 ccgcttcagc gcgtaatatc cgggtatttc ccacgtccgc gcgcatgccg tttgataacg
     4801 tgtagtcctg accatctgtg gtaaagccgg taaaggccag atagggtcta acactccaca
     4861 atccgtcaac ccaacggaac ccgctctcaa catgagcacc cgcgccgtta ctattgtaat
     4921 cgccaaacgc tgttgcccca ttactcatct tgccatggat ggtgttggca aaacggtcaa
     4981 ctttcaccac cccatcaaca taggcaccgt tctgatgctc ccaaccggca taagccccca
     5041 gggtatagct atcgatatta ccttttccgc cgcgatcaaa accaatatca gaatgagagt
     5101 aaccaaagaa caagccgcga attgtactgc tttcttcacg ggagaaacgg ctatcgatac
     5161 cgagcgtcag gcccgtcaat gtttgctcaa aaccagctcc cgcatcagtg gtcacgttgt
     5221 tgcgggtgtt aattgccgaa ctccacatcg ccgtatcgta actaacgcct tttacgctac
     5281 caagacgctc acgcacggtg tccagttctg catcaaatac cagcggttgt gctgccgcca
     5341 tatttagcac atcagtggtt gaaggggtaa tttgcgcgcg attctctgcc agactccagc
     5401 tatggttacc attatccagc aaggtatatt catacgtacc gatatcaaca acgcctccgg
     5461 cattgcccaa cgtaaatgca gcatcaccgc cgcccgttgt taccagtgta aggctatctc
     5521 ctgctgccgg gctggcaccg gtgtccgtca cgaatatttt gaaatcacct gttgcctgac
     5581 cggtgacgtt gagctgatcg ctctgatgat tagctatatc ggtacgcata taaaaattgc
     5641 cgtttcccga cagggtattg gtggtcaacg taatgaaatt ttccgcactt gccgtcactg
     5701 atgaacgagt cactggctca tccatcataa tcacatttgc gtcagacatc gcaaggttgg
     5761 ctaagttgag gagggtgttt tggttcgtcg tagtgatatc ggcaatgatg gtccatacgc
     5821 tattttcgag cgaaacgtcc gctttgctgg tatccacggc gccatgcgtt ctaaaagaag
     5881 cgccctcaga aaccactacc ttacccacca acattgcggg tgcataattt atttgcggct
     5941 tcatgacctc aagaatgcca tcattccctc tgactgaaac gttaaccatt gtgccagccc
     6001 agacgttagc gcggccattg ttgataacca tattttcaga gatggctttt gaggaatacg
     6061 tgatacttcc attctgatct gatcgaccga gatcatatac cgcatttgcc tgcactctcg
     6121 tcccagtatc ctttcccagc gattgcatag tggcatgctc atcaaggata gtgtcaatgg
     6181 cctgcgtgtc ctccataaca ataagcccgg aaccatcatc cagttcataa ttttttgaca
     6241 caccatcttt tatactaaat tggcctttac tgtttgtacc actcacttcc agcgcattcg
     6301 ttgagacaat taattttccg cctgcttttt tatcaacgcc agttgcagtc ccgccatcca
     6361 taacctccag taagccgcca ctatctacag tagtattata agcgaagtca ttttcttcaa
     6421 ctcgcaaact tccgccgttt tccaacagca tattgtttgc aattccattc ttgatatcga
     6481 actgaccgag acggtttgtt ccgaatacct ccatgacccg cgtggttgct ttaatagcac
     6541 cgcctgcttt ctgatctacc gcaatcgcta atcccccctc tttaacctcc agtaatcctc
     6601 ccttttcgat agcggcaagg acggtaatcc catctttttc aacgattaag cgcccgcctt
     6661 taatcgtggg atcattcgcc gcgccataaa cctgtagcac accattattg agggtagtct
     6721 ggtttgccgc gccacccatt tgaacaattt gataaccatc atttaatacg gagtattctg
     6781 cggtgccatt catttcaatg tactgatacc cgccgttaat ttcagtatta ctacttatac
     6841 caaattcttt aatatgctgt tctccgccag gattgatttt cgtattattg gcggtcccat
     6901 aaaccagctg gatatctttt tccaggacaa caccatcaac tgtctcacca tactcaactg
     6961 ttgatgccaa tgctatgggt gaatataacg ctgtagccac cagaacagat attgcggaac
     7021 gacaaagaga aacagaacca gattgatgca ttgagctttc atcctatgaa attaattgct
     7081 gttaaaagca ttgggtacag aaaataccca tagctccata cccggaatca gtttttaaaa
     7141 actgtttaaa gaaatgcaca agtattgtga ttgatttttt agttgttttt cttgatgaga
     7201 agctgatgca aaattccgtc tttataatga aaatgatgcc aaagcgaacg gcaaggttgt
     7261 agttttcact acatgtccat acataaaatg gggtaacatt cacgcgcctg gtagcgttac
     7321 caacgctacg ctcaaacata atgattctaa taaaacctca ggagactact atgcctgaag
     7381 caacgccttt tcaggtgatg attgtggatg atcatccact tatgcgacgc ggtgttcgtc
     7441 agttactgga gcttgatcct ggctttgaag tggtcgccga agcgggcgac ggcgcgagcg
     7501 cgatcgatct ggcgaataga ctggatatcg acgtgatctt gctggatctg aatatgaaag
     7561 gtatgagtgg cctggatacc ctcaatgcct tgcgcaggga tggcgttacc gcgcaaatta
     7621 ttatcttgac cgtatcagat gcctccagcg atgtctttgc actgatagac gcaggcgcag
     7681 acggttatct gttgaaagac agcgacccgg aagtgttgct ggaagcgatt cgtgccggag
     7741 cgaaaggcag caaagtcttt agcgaacgcg tcaatcagta cttacatgaa cgtgaaatgt
     7801 ttggcgcgga agaagatccc ttcagcgtgc tgacggagcg cgagctggat gttctgcacg
     7861 agctggcaca ggggctgtca aataaacaga ttgcctcggt gttgaatatt tcagagcaga
     7921 cagtaaaagt acatattcgc aatctgctgc gcaaactcaa tgtccgctca cgcgtggcag
     7981 ccaccattct gttcctgcaa caacgcgggg cacaataaaa atagcccgat ggcatcacca
     8041 tcgggcctct ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact
     8101 acgttcaatc accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca
     8161 cgcggcaacg gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttaa
     8221 cacacgaata ttgctatggt ccgttctcac caactggcgc agcagttcgc cgccgaggcg
     8281 gttgtcgttg ggatcagatg aacgtgtcaa cgcttcagca taacccagtg cggcatcgct
     8341 gtttttcgga tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc
     8401 cagcgccatg ccaacgcgac ccaacataat ccaaccttct atatctcccg gatttttttg
     8461 cagttgagta cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc
     8521 tttcggatcc agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat
     8581 tttcacctgc tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac
     8641 cacaataccc ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc
     8701 aggaaacgct tccagcacca cgcgtacccg acgccgcgag cgggcgtaaa tgacccaacc
     8761 gccaatgcca atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg
     8821 cggatcgtaa gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttctt
     8881 acttttacct tcctgcatca gttcatacac tttctggcgc aggtcggtgg caatcatcga
     8941 gttggaatcg gcaatgctgt tattctgaca tttcgggcag cgcagttctt cagtgagctg
     9001 acggaactgt tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc
     9061 tgagccggag atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta
     9121 ctgtatttct cccacagcgg cttgatctct tcttcccaga cgcgaggatt cagatcgccc
     9181 gcatggcgat agcgaatgat gccgttgccg tcaataagga acgtttctgg cgcgccatag
     9241 acaccgagat ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg
     9301 cccagctctt tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc
     9361 acgcggatgc cctgcgcaga aagctgattc agatattgat gttccgcacg gcaggtcgga
     9421 caccaggtcg cccagacgtt aagcagcact ggtttgccct gagtcagcac atccgcctga
     9481 taaaactgcc ccggattgtc cagtgattcg aggcgaaact tcggcacagg cttgccaatg
     9541 agcgccgatt ccagattggt cggatcatcc ccttcggcat tacgcgccaa ctgccacagc
     9601 agcgccgcgg caatcgccag gaagataatc aaaggaatta acaatacttt gcgcttcata
     9661 cagcctccgg cgcagttttc tgcgggttca cgcgcttacg atagcgagga tcaaacaggc
     9721 acagcagc
//
LOCUS       DAZFKF010000150         9722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFKF010000150 DAZFKF010000000
VERSION     DAZFKF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.152"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(421..3093)
                     /gene="rcsD"
                     /locus_tag="ACHKA1_004726"
     CDS             complement(421..3093)
                     /gene="rcsD"
                     /locus_tag="ACHKA1_004726"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO5682276.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVNIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            3832..4959
                     /gene="ompC"
                     /locus_tag="ACHKA1_004727"
     CDS             3832..4959
                     /gene="ompC"
                     /locus_tag="ACHKA1_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO5682277.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNAPESENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGYATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNGSYISNGVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLG
                     WANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYY
                     FNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            5071..6126
                     /gene="apbE"
                     /locus_tag="ACHKA1_004728"
     CDS             5071..6126
                     /gene="apbE"
                     /locus_tag="ACHKA1_004728"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO5682278.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            6200..7264
                     /gene="ada"
                     /locus_tag="ACHKA1_004729"
     CDS             6200..7264
                     /gene="ada"
                     /locus_tag="ACHKA1_004729"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO5682279.1"
                     /translation="MKNATRLTEDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYADASEALAAGFRPCKRCQPDKANAQQHRLDKITRACRLLEQEMPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRYGGENLAVRYALADCEQGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            7264..7914
                     /gene="alkB"
                     /locus_tag="ACHKA1_004730"
     CDS             7264..7914
                     /gene="alkB"
                     /locus_tag="ACHKA1_004730"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO5682280.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            7990..9633
                     /gene="yojI"
                     /locus_tag="ACHKA1_004731"
     CDS             7990..9633
                     /gene="yojI"
                     /locus_tag="ACHKA1_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO5682281.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         2383 a         2349 c         2513 g         2477 t
ORIGIN      
        1 ataactgaag catccgacga cgtagtgtct accagagtgt cagatatgca ggaaaatatg
       61 agttccgaca gaaatgtagt aaaaaaagga gtctcgcact cccctccata aaactccagg
      121 gctgtaagtt gtattaaaaa gctgattttt ctttagcgat acagacctca ataagtataa
      181 cacccttcag ttactgtggt tatgcacagg tgttattttt aacagtgtgg ataaacagtg
      241 tcgattattc agattggtta tggggcatgt atgggacatt tttaatcggc gaaatacgtc
      301 gaagttcgtc gatatggtaa acgaatcatc tatccaaccc ttgaaaaacg gcgctcctgg
      361 acgatcttcg tcgattttta aaatgcaaca agtacgttgt tcatgtaata ggctaccttg
      421 ctacagcaag ctcttgacat aactgtcaat gtcgctgatg tatttttcta ttcctggaac
      481 atccttctca cgaatcagat gttccagcgt ttcacataac tgcttgccgg gtaccagatt
      541 tagcatggca aatacgcctt taagacgatg agccgtttgg gctaacgcag caaagtcact
      601 ggttgctgct tcagtataca gcctcttaac atcatccggt actgtgtcta caaagagcgc
      661 ataatagccg ctggcatgga gttgcgcatt ttcatctccg cccagaggcg attctgtcac
      721 ctcttcctgc gccagttgca cttcaattaa ttgtaagacc gcttcctgca tagcgttgct
      781 catattgaag ttgacgcaca attgaccagg cccaatttcc cgtacgccag actcatcatc
      841 gcttaaaagc aagccagagg cagtaagatt agacggatta tccgttaaaa agatatcata
      901 atcttgacta attaatcttt catcgggtgt gatacaggtt gcaccccaat tttctaactg
      961 gcgagtgaca atattccgaa tttctgccga agtaacatcc accattacgc agacatcatc
     1021 cagtaaacgc tcttcttcct cttcaacttc cgggtcagct gcgagcattt tgatatgcac
     1081 agagtagcgt gtaccaagcc catcccgcgt tttgatgttt aaatgaccgc ccagtttacg
     1141 tgccagttga tcgctcagcc agaatgccag cgggtccgcc ttgccatagc gatcgttttg
     1201 ggtctggttg ataaacggga agtgcaaatt atccatttca tgaatgttta cgccttctcc
     1261 ggtgtccagg atgcggaacg tcaggcggtc ttcggaggac tcatcctgat caacctcaag
     1321 ggtgattttt cccaattgcg ttgaggtcac ggcatattgc atcagtagca gcaaaatacg
     1381 tcgtaaggca tcgcgatcgc cgcggcgcat atcgtgtgct ttcagatgat tgttaatcag
     1441 cagttgcaga cctttacgct tgatggcagg caacactgaa ggcacaactt catcaattaa
     1501 atcctgcacg gagaacagca ccgtctcact tttccagcta tcgtccgcaa gcatgttcgc
     1561 taactgtatt tcatcgacca gccgcaccag cacatctgcc tgattcgcca gttgtttgct
     1621 ttccggggcg ttgagtttag ccgcgctctc cgccagggac tgtgcaggtt ctttcagcgc
     1681 atcgccaatg tttttcataa aggtcatccg cccctgctgg tttttctcat acagacgctg
     1741 cgcctgcttg agtttcttgt ttaccagcac ttcgcgatcc tgatcgcgaa taatgaaaat
     1801 ttgtgtgcgc ggcgcgacct ggctgcggaa catgcggatc tcatacagct cgttattgat
     1861 cgtcgcctga ataatcccct gatgctgttc cgccatggtg gtgatgtttt gcagattcaa
     1921 atgcggcagc aaatgatcgg caattttgtt acttatgaca gtgcggttcg attcctgatc
     1981 gtgaaccagc aggccgagcg gcagcagtga gactatctct tcattgattg cccgtaaaat
     2041 gcgcaattcg ttattgaccg ccgtgttggg tagactttct gtactgcgac tggagaaatg
     2101 gcggaatgtg gtatagccaa ataacgccag cgccagcaaa ccgatgttca gcagcagtgg
     2161 cagcagaatg ttttgcaacg tatccagcaa taaggtgcca taaggaacct gccagaccag
     2221 gcgcatatcg gtagagttga gtgccgagga gatttcaatc ttcgtactgt taaagtggat
     2281 actgacgcta tccgtccctt ctttctcatt atcattgtct cccgttgccg tcgcgtctgg
     2341 ctcaaggcgg aaactgtcca gcggcatacc cggtgggatc aaatcattaa tcggcagatc
     2401 aaaagccacg accgttgcca gatgtcccgg ctggttaaag gtggtacgca aggtaaagta
     2461 atgaccgttc tgccaggcca ggcggcgcat gttagaaaag ctttcgcgtt catcgagagc
     2521 gttggcctgt tgcaacatct ctgcacgacg tgaatcaaca atgtcactga cggtcgattc
     2581 tttaaatccg gaggtgagat ctttgagggg cagggttgag atcagcacca gactgttatc
     2641 ctgaccattc aggtaataca ttgaccacgg tacattttct gcgccccaca atgtgtccag
     2701 ataggtggac atccgttgag tcatctcaag cgttgagttg tcgtgagagc caaagattaa
     2761 cgcttccgtt ttgcggcgcg gtttttccag atagtagaca tcctgtttca ggcgcgtctc
     2821 ttgtaaacct tcgccggagg agggggaggt cgtcgcggca atgttgtcgt agatctgcca
     2881 ggtcacgtaa cgccaggtat cgacgcgctt ttggatagcg tgggtaatgt cgacaatctg
     2941 gtaactttta tctttcagcc aggcgttaac ggcgctttgt accattacac ccatcgtcac
     3001 cagtaacaca atgatcaaca gtaaaaagaa gcgggtaatg ctccccggta ggagtgaaaa
     3061 gcgggtcgtg gccgttgtct ctttctgacg cattcgtgtt tatgacctgt taaaacttcg
     3121 cgaagtgata gggcagtata aagggtacag tgtgaaattc cagactctta cctgattgat
     3181 gattctctat tctcccgtga tatttatcat cctgcattgg gggaaataga tgtacaagcg
     3241 ccattttgct gtacaaataa cctacaaaaa gcccaacgaa aataattgca aataaacaaa
     3301 gattgctgga aattatgctg atgttaatta tttgtgaaat ggttaacaag cgttatagtt
     3361 tttctctggt agcacagaat aatgaaaagt gtgtaaagaa gggtaaaaaa aaccggatgc
     3421 gaggcatccg gttgaaatag gggtaaacag acattcagaa atgaatgacg gtaataaata
     3481 aagttaatga tgatagcggg agttattcta gttgcgagtg aaggttttgt tttgacattc
     3541 agtgctgtca aatacttaag aataagttat tgattttaac cttgaattat tattgcttga
     3601 tgttaggtgc ttatttcgcg attccgcaat aatcttaaaa agttcccttt tatttacatt
     3661 ttgaaacatc tatagcgata aatgaaacat cttaaaagtt ttagtatcat attcgtgttg
     3721 gattattctg catttttggg gagaatggac ttgccgactg attaatgagg gttaatcagt
     3781 atgcagtggc ataaaaaagc aaataaaggc atataacaga gggttaataa catgaaagtt
     3841 aaagtactgt ccctcctggt cccagctctg ctggtagcag gcgcagcaaa cgctgctgaa
     3901 gtttacaaca aagacggcaa caaattagat ctgtacggta aagtagacgg cctgcactat
     3961 ttctctgacg acaagtctgt agatggcgac cagacctaca tgcgtcttgg cttcaaaggt
     4021 gaaactcagg ttactgacca gctgaccggt tacggtcagt gggaatatca gatccagggc
     4081 aacgctccgg aaagcgaaaa caactcctgg acccgtgtgg catttgctgg tctgaaattc
     4141 caggatgtgg gttctttcga ctacggtcgt aactacggcg ttgtttatga cgtaacttcc
     4201 tggaccgacg tactgccaga atttggtggt gacacttacg gttctgacaa cttcatgcag
     4261 cagcgtggta atggctacgc aacctaccgt aacaccgact tcttcggtct ggttgacggt
     4321 ctgaactttg ctgttcagta ccagggcaaa aatggtagcg taagcggcga aggcatgacc
     4381 aacaatggtc gtggtgctct gcgtcagaat ggcgacggtg tcggcggatc tatcacttat
     4441 gattacgaag gcttcggtat cggtgctgca gtttccagct ccaaacgtac tgatgatcaa
     4501 aatggtagct acatcagcaa tggtgtagtt cgtaactaca tcggtactgg cgaccgtgct
     4561 gaaacctaca ccggtggtct gaaatacgat gctaacaaca tctacctggc tgctcagtac
     4621 acccagacct acaacgcaac tcgcgtaggc aacttgggtt gggcgaacaa agcacagaac
     4681 ttcgaagctg ttgctcagta ccagttcgac ttcggtctgc gtccgtccct ggcttacctg
     4741 cagtctaaag gtaaaaacct gggtgtcatc aatggtcgta actacgacga cgaagatatc
     4801 ctgaaatatg ttgatgttgg tgcgacctac tacttcaaca aaaacatgtc cacctacgtt
     4861 gactacaaaa tcaacctgct ggacgacaac cagttcactc gtgacgctgg catcaacact
     4921 gataacatcg tagctctggg tctggtttac cagttctaat ctcgattgat atcgaacaaa
     4981 gggcctgcgg gccctttttt cattgttttc agcgtacaaa ctcagttttt tggtgtactc
     5041 ttgcgaccgt tcgcatgagg ataatcacgt atggaaataa gctttacccg cgtggcactg
     5101 ctggctgccg cgctcttctt tgttggttgc gatcaaaaac cacaacccgc caaaacccac
     5161 gctactgaag ttaccgttct tgaaggcaaa accatgggta ccttctggcg tgccagcatc
     5221 ccgggcattg atgccaaacg cagtgccgaa cttaaagaaa agattcagac ccagctggac
     5281 gccgacgatc agctgctttc gacctataaa aaagattccg cgctgatgcg ctttaacgac
     5341 tcgcaaagtt tgtcgccgtg gccagtaagt gaagcgatgg ccgaaatcgt cactacctcg
     5401 ctgcgcattg gcgcgaagac cgatggtgcg atggatatta ccgtcgggcc gctggtgaat
     5461 ctgtggggct ttggcccgga acaacagccg gttcaaattc cgagccagga acagatcgat
     5521 gcgatgaaag ccaaaaccgg cttacagcac ctgacggtca ttaatcagtc gcatcagcaa
     5581 tatctgcaaa aagacctgcc ggatttatat gtcgatctct ctaccgtcgg cgaaggttat
     5641 gcggcggatc acctggcacg cttgatggag caggaaggga tttcccgcta tctggtgtcg
     5701 gtgggcggcg cgctgaacag ccgtggtatg aacggtgaag gccagccgtg gcgggtagcg
     5761 attcaaaagc caaccgataa agaaaatgcg gttcaggccg tggtggatat caacggtcat
     5821 ggtattagca cctctggcag ctaccgtaac tattacgaac tggacggcaa acgtctttcc
     5881 catgttatcg atccgcaaac cgggcgtccc atcgaacaca atctggtatc cgtgacggtg
     5941 attgctccga cggcgctgga agccgatgcc tgggatactg gcttgatggt actcgggccg
     6001 gagaaagcca aagaggttgt tcgccgggaa gggctggcgg tttatatgat caccaaagag
     6061 ggcgatagct ttaaaacctg gatgtcgccg cagtttaaaa gcttcctggt gagcgaaaaa
     6121 aattaaagcg caagattgtt ggtttttgcg tgatggtgac cgggcagact aaaggctatc
     6181 cttaaccagg gagctgatta tgaaaaacgc cacacgctta actgaagatc aacgctggca
     6241 atctgtctta gcccgcgacc cgaatgccga cggcgaattc gttttcgccg tgcgcaccac
     6301 aggtatcttt tgccgtccgt cttgccgcgc cagacatgcc ttgcgggaaa acgtttcttt
     6361 ctatgctgat gccagcgagg cgcttgccgc tggctttcgt ccctgcaaac gctgccaacc
     6421 tgataaagcc aacgcccagc aacatcggct ggataaaatc acccgcgctt gtcgactgct
     6481 ggaacaggaa atgcctgtaa cgctggaggc cttagctgac caggtggcga tgagtccgtt
     6541 ccatctgcat cgtttattta aagccacgac cggaatgacg ccaaaagcct ggcaacaggc
     6601 ctggcgcgct cgccgtttgc gcgaatcgct ggcgaaaggg gagagcgtga cgacgtctat
     6661 tcttaacgcc ggattccccg acagcagcag ttactaccgc aaagccgatg aaacgctggg
     6721 catgacggct aaacaattcc gttacggtgg cgaaaatctg gcggtgcgtt acgcgctggc
     6781 tgattgtgag cagggtcgtt gcctggtggc agaaagcgag cgggggattt gcgcgatatt
     6841 gctgggcgat gatgacgcga cactaatcag cgagttgcag cagatgtttc ccgctgccga
     6901 caacgcgcct gccgatctga cgtttcagca acatgtgcgt gaagtgatcg ccagcctcaa
     6961 tcaacgcgat acgccgctga cgttaccgct ggatattcgc ggcactgctt ttcagcaaca
     7021 agtctggcag gcactgcgca cgataccttg cggtgaaacc gtcagttatc agcaactggc
     7081 taacgccatc ggcaaaccga aagcggtacg ggccgttgcc agcgcctgtg ccgccaacaa
     7141 gctggctatc gtaataccct gtcatcgagt ggtccgtggt gatggcacac tttccggtta
     7201 ccgctggggc gtgtcgcgta aagcgcaact gctgcgccgc gaagctgaaa atgaggagag
     7261 gtaatgttgg atctgtttgc cgatgctgaa ccgtggcaag agccactggc ggctggtgcg
     7321 gtaattttac ggcgttttgc ttttaacgct gcggagcaac taatccgcga tattaatgac
     7381 gttgccagcc agtcgccgtt tcgccagatg gtcactccag gcggatatac catgtcggtg
     7441 gcgatgacca actgtgggca tctgggctgg acgagccatc ggcaaggtta tctctattcg
     7501 cccattgatc cgcaaacaaa taaaccgtgg cccgccatgc cgcagagttt tcataattta
     7561 tgtcaacgtg cggctacggc ggcgggctat ccagatttcc agccagatgc ttgtcttatc
     7621 aaccgctacg ctcctggcgc gaaactgtcg ctgcatcagg ataaagacga accggatctg
     7681 cgcgcgccaa ttgtttctgt ttctctgggc ttacccgcga tttttcaatt tggtggcctg
     7741 aaacgtaatg atccgctcaa acgtttgttg ttggaacatg gcgatgtggt ggtatggggc
     7801 ggtgaatcgc ggctgtttta tcacggtatt caaccgttga aagcggggtt tcatccactc
     7861 accaccgact gccgctacaa cctgacattc cgtcaggcag gtaaaaaaga ataaaaataa
     7921 gaattattat tgctgtgcgc gcgaagatgt ttaaactgcg ggctgtaatt cattgtccgg
     7981 gttttctgca tggaacttct tgtacttgtc tggcggcagt atcgctggcc atttatcagt
     8041 gtgatggcgc taagcctcgc cagtgcggca ttaggcattg gcttaattgc ttttatcaac
     8101 cagcgcctta tcgaaacggc ggataccagt ctgctggtgt tgccggagtt tctgggatta
     8161 ttgctgctgt tgatggcagt cactctcgga tcgcaactgg cgctcaccac tttggggcat
     8221 cacttcgttt accgactgcg tagtgaattt atcaagcgga ttctggatac ccacgtcgag
     8281 cgcattgaac aactcggtag cgcctcgttg ctggcgggat taaccagcga tgtgcgcaat
     8341 atcaccattg cttttgtgcg tctgccggaa ctggtgcagg ggatcattct cactatcggt
     8401 tcagcggcgt atctgtggat gctgtcgggc aaaatgttgc tggtaacggc tatctggatg
     8461 gcagtcacca tctggggcgg ttttgtgctg gtggcgcggg tgtacaaaca tatggcgacc
     8521 ctgcgtgaaa ccgaagacaa gctgtacacg gattttcaaa ctgtacttga agggcgcaaa
     8581 gagctgactc tgaaccggga acgcgccgag tatgtgttta acaacctcta cattcctgat
     8641 gcgcaagagt atcgccatca tattattcgc gcagacacct tccatcttag tgccgtgaac
     8701 tggtcaaaca tcatgatgct gggcgcaatc ggcctggtgt tctggatggc gaacagcctc
     8761 ggttgggctg ataccaacgt tgccgcgacc tattcgttga cgcttttatt cctgcgtacg
     8821 ccgctgcttt cggcggttgg cgcattgccg acgctgctga cggcgcaggt ggcgtttaac
     8881 aagctgaaca aattcgcgct cgcgcctttc aaagcagagt ttccgcgccc gcaggcgttt
     8941 cccaactggc aaacgctgga gctgcgtaac gtgacgtttg cttatcagga taacgcgttt
     9001 tccgttggtc cgattaatct caccatcaaa cgtggcgagc tgctgtttct gattggcggc
     9061 aacggtagcg gaaaatcgac gctggcgatg ttgttgacgg gcttgtatca gccacaaagc
     9121 ggcgaaatct tgctggatgg caaacctgtc agcggcgaac aaccggaaga ttatcgcaaa
     9181 ctgttttcgg cagtgtttac cgatgtctgg ctgtttgatc aactgctggg gccggagggt
     9241 aaacccgcta acccgcaact ggttgagaag tggctggcgc agctgaaaat ggctcataag
     9301 cttgagttaa gcaacgggcg tattgttaac ctgaagttat caaaagggca gaaaaaacgc
     9361 gtggcgctgt tgctggcgct ggcagaagaa cgcgatatta tcctgctgga tgaatgggcg
     9421 gcggatcagg atccacactt ccgtcgtgag ttttatcagg tgttgctgcc gctgatgcag
     9481 gagatgggta aaactatttt cgccatcagt catgatgatc attactttat ccacgccgac
     9541 cgcctgctgg agatgcgcaa cgggcaactt agcgagctga cgggcgaaga gcgcgatgcc
     9601 gcttcgcgtg atgccgttgc ccggacggca taatattacg acgctgacct ggcggactgc
     9661 tgccgccagg tcaatatgct aaaaatccct tcatttctaa taaccctata attaatcaac
     9721 ga
//
LOCUS       DAZFKF010000151         9596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFKF010000151 DAZFKF010000000
VERSION     DAZFKF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.153"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            255..329
                     /gene="tnaC"
                     /locus_tag="ACHKA1_004732"
     CDS             255..329
                     /gene="tnaC"
                     /locus_tag="ACHKA1_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO5682282.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            550..1965
                     /gene="tnaA"
                     /locus_tag="ACHKA1_004733"
     CDS             550..1965
                     /gene="tnaA"
                     /locus_tag="ACHKA1_004733"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO5682283.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            2056..3303
                     /gene="tnaB"
                     /locus_tag="ACHKA1_004734"
     CDS             2056..3303
                     /gene="tnaB"
                     /locus_tag="ACHKA1_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO5682284.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3435..4610
                     /gene="mdtL"
                     /locus_tag="ACHKA1_004735"
     CDS             3435..4610
                     /gene="mdtL"
                     /locus_tag="ACHKA1_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO5682285.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSQAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4585..5544
                     /gene="yidZ"
                     /locus_tag="ACHKA1_004736"
     CDS             4585..5544
                     /gene="yidZ"
                     /locus_tag="ACHKA1_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO5682286.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPSGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5701..6450
                     /gene="yieE"
                     /locus_tag="ACHKA1_004737"
     CDS             5701..6450
                     /gene="yieE"
                     /locus_tag="ACHKA1_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO5682287.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKAFSPN"
     gene            6472..7038
                     /gene="yieF"
                     /locus_tag="ACHKA1_004738"
                     /gene_synonym="chrR"
     CDS             6472..7038
                     /gene="yieF"
                     /locus_tag="ACHKA1_004738"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO5682288.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQEEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTNSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(7092..8429)
                     /gene="adeP"
                     /locus_tag="ACHKA1_004739"
     CDS             complement(7092..8429)
                     /gene="adeP"
                     /locus_tag="ACHKA1_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO5682289.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8596..9261
                     /gene="yieH"
                     /locus_tag="ACHKA1_004740"
     CDS             8596..9261
                     /gene="yieH"
                     /locus_tag="ACHKA1_004740"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO5682290.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGINVDLEEIFT
                     RFKGVKLYEIIDIISKEHGVTMVQADAEHVYRAEVARLFDTELADIAGANELLASVNV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWN
                     ITR"
     gene            complement(9398..>9596)
                     /locus_tag="ACHKA1_004741"
     CDS             complement(9398..>9596)
                     /locus_tag="ACHKA1_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682291.1"
                     /translation="VQDEVNALNAKIVDCDDKIRSKLTIIRNNRLENQYKDDKNISDA
                     MRNILDWFEQYSDIVKNITKA"
BASE COUNT         2385 a         2372 c         2359 g         2480 t
ORIGIN      
        1 aaaccgggac gtcaatgtgg gaaacagctc cttgtaatgg aacccctata atcaatgcca
       61 tcatcttcaa caataatgat aaaaattaaa aaatttatcc attcatggat ttttgctaat
      121 attttgtatg ggccagctat cacaaacaca gccaatatct aattgtgatt cgattcacat
      181 ttaaacaatt ttagaataga caaatttctt gagtattata ctgtagcctc gtgtctttcg
      241 aggataagtg cattatgaat atcttacata tatgtgtgac ctcaaaatgg ttcaatattg
      301 acaacaagat tgtcgatcac cgcccttgat ttgcccttct gtagccatca ccagagccaa
      361 accgattaga ttcaatgtga tctatttgtt tgctatatct taatttttcc ttttgcaaag
      421 gtcatctctc gtttatttac ttgtgttagt aaatgatggt gcttgcatat atacatggcg
      481 aattaatgga tattgcaaat gtaatattca cagggatcac tgtaattaaa ataaatgaag
      541 gattatgtaa tggaaaactt taaacatctc cctgaaccgt tccgcattcg tgttattgag
      601 ccagtaaaac gtaccactcg cgcttatcgt gaagaggcaa ttattaaatc cggtatgaac
      661 ccgttcctgc tggatagcga agatgtgttt atcgatttac tgaccgacag cggcaccggg
      721 gcggtgacgc agagcatgca ggctgcgatg atgcgcggcg acgaagctta cagcggcagc
      781 cgcagctact atgcgttagc cgagtcagtg aaaaatatct ttggttatca atacaccatt
      841 ccgactcacc agggccgtgg cgcagagcaa atctatattc cggtactgat taaaaaacgc
      901 gagcaggaaa aaggcctgga tcgcagcaaa atggtggcgt tctctaacta tttctttgat
      961 accacgcagg gccatagcca gatcaacggc tgtaccgtgc gtaacgtcta tatcaaagaa
     1021 gcattcgata cgggcgtgcg ttacgacttt aaaggcaact ttgaccttga gggattagaa
     1081 cgcggtattg aagaagttgg tccgaataac gtgccgtata tcgttgcaac catcaccagt
     1141 aactccgcag gcggtcagcc ggtttcactg gcaaacttaa aagcgatgta cagcatcgcg
     1201 aagaaatacg atattccggt ggtaatggac tccgcgcgct ttgctgaaaa cgcctatttc
     1261 atcaagcagc gtgaagcaga atacaaagac tggaccatcg agcagatcac ccgcgaaacc
     1321 tacaaatatg ccgatatgct ggcgatgtcc gccaagaaag atgcgatggt gccgatgggc
     1381 ggcttgctgt gcatgaaaga cgacagtttc tttgatgtgt acaccgagtg cagaaccctt
     1441 tgcgtagttc aggaaggctt cccgacatat ggcggcctgg aaggcggcgc gatggagcgt
     1501 ctggccgtag gtctgtatga cggcatgaat ctggactggc tggcttatcg tattgcgcag
     1561 gtgcagtatc tggtcgatgg tctggaagag attggcgttg tctgccagca ggcgggcggt
     1621 cacgcggcat tcgttgatgc cggtaaactg ttgccgcata tcccggctga tcaattcccg
     1681 gcacaggcgc tggcctgcga gctgtataaa gtcgccggta tccgcgcggt agaaattggc
     1741 tctttcctgt taggccgcga tccgaaaacc ggtaaacaac tgccatgccc ggcagaacta
     1801 ctgcgtttaa ccattccgcg cgcaacatat actcaaacac atatggactt cattattgaa
     1861 gcctttaaac atgtgaaaga gaacgcggcg aatattaaag ggttaacctt tacctacgaa
     1921 ccgaaagtat tgcgtcactt caccgcaaaa ctgaaagaag tttaattaat actacagagt
     1981 ggctataagg atgttagcca ctctcttacc ctacatcctc aataacaaaa atagccttcc
     2041 tctaaaggtg gcatcatgac tgatcaagct gaaaaaaagc actctgcatt ttggggtgtt
     2101 atggttatag caggtacagt aattggtgga ggtatgtttg ctttacctgt tgatcttgcc
     2161 ggtgcctggt ttttctgggg tgcctttatc cttatcattg cctggttttc aatgcttcat
     2221 tccgggttat tgttattaga agcaaattta aattatcccg tcggctccag ttttaacacc
     2281 atcaccaaag atttaattgg taacacctgg aacattatca gcggtattac cgttgccttc
     2341 gttctctata tcctcactta tgcctatatc tctgctaatg gtgcaatcat tagtgaaacg
     2401 atatcaatga atttgggcta tcacgctaat ccgcgtattg tcgggatctg cacagccatt
     2461 ttcgttgcca gcgtattgtg gataagttcg ttagccgcca gtcgtattac ctcattgttc
     2521 ctcgggctga agattatctc ctttgtgatc gtgtttggtt ctttcttctt ccaggtcgat
     2581 tactccattc tgcgcgatgc caccagcacc actgcgggaa cgtcttactt cccgtatatc
     2641 tttatggctt tgccggtgtg tctggcgtca tttggtttcc acggcaatat tcccagcctg
     2701 attatttgct atggcaaacg caaagataag ctaatcaaaa gcgtggtctt cggttcgctg
     2761 ctggcgctgg tgatttatct cttctggctc tattgcacca tggggaatat tccgcgagaa
     2821 agctttaagg cgattatttc ctcaggcggc aacgttgatt cactggtgaa atcgttcctc
     2881 ggcaccaaac agcacggcat tatcgagttt tgcctgctgg tgttctccaa cttagctgtc
     2941 gccagctcgt tttttggtgt cacgctgggg ttgttcgatt atctggcgga cctgtttaag
     3001 attgataact cccacggcgg gcgtttcaaa accgtgctgt taaccttcct gccacccgcg
     3061 ttgttgtatc tgatcttccc gaatggcttt atttacggga tcggcggtgc cgggctgtgc
     3121 gccactatct gggcggtcat tatccccgcg gtgctggcaa taaaagctcg caaaaagttt
     3181 cccaatcaga tgttcacggt ctggggcggc aatcttattc cggcgattgt cattctcttt
     3241 ggtataaccg taattttgtg ctggttcggc aacgtcttta acgtgttacc taaatttggc
     3301 taaatccttc aagaagccag tcattcgctg gcttcttgcc tctcaggaaa tcacttatgt
     3361 ccaaatggca actcgcctga tcctctttca ccacgtatgc tttgcgtcac cttactatca
     3421 ggacgcttta gcccatgtcc cgctttttga tttgtagttt tgccctggtt ttactttatc
     3481 ccgctgggat tgatatgtac ctcgttggtt taccgcgcat cgccgccgat ctcaatgcca
     3541 gcgaagcgca gttacatatt gcgttctccg tttatctggc aggtatggca gctgcgatgt
     3601 tatttgccgg taaagtggcc gatcgttcag ggagaaaacc ggtcgccatc cccggcgcgg
     3661 cgctatttat tatcgcttcg gtgttctgtt cacaggctga aaccagtacg ttatttcttg
     3721 cagggcgctt tctacagggg ttgggcgcgg gctgttgtta cgtggtggca ttcgccattt
     3781 tgcgcgacac gctggatgat cgacgtcggg caaaagtgct gtcgttactc aacggcatta
     3841 cctgcatcat tccggtgtta gcgccggtgc tcggtcatct gattatgctt aaattcccgt
     3901 ggcagagtct gttctggacg atggcaataa tgggcatcgc ggtactgatg ttgtctttgt
     3961 ttattttaaa agaaacgcgc cctgccgccc ccgcagcttc ggacaaatca cgagaaaata
     4021 gcgagtcgct gcttaatcgg tttttcctca gccgtgttgt tatcaccacc ctcagcgttt
     4081 cggtgatcct caccttcgtc aacacgtcgc cggtattgct gatggaaatc atgggttttg
     4141 agcgcggaga atacgccacc attatggcgt tgaccgctgg cgtcagcatg accgtttcat
     4201 tctcgacgcc atttgcgctg ggaattttta agccacgtac gttgatgatc acctcacagg
     4261 tgttattcct ggcggcgggg atcactcttg ccgtttcacc ttcccatgcg gtttctctgt
     4321 ttggtatcac gctgatttgc gccggtttct cggttggttt tggtgtggcg atgagtcagg
     4381 cgttagggcc gttttcatta cgcgcgggtg tagccagctc gaccttaggt attgcgcagg
     4441 tttgcggttc gtcactgtgg atttggctgg cggcggtggt tggtatcggc gcatggaata
     4501 tgctgatcgg gattctgatt gcatgtagca tagtgagcct gttgctgatt atgttcgtcg
     4561 cgcctggacg ccccgttgcc gctcatgaag aaatccatca ccacgcttga tctcaatctg
     4621 ctgctctgtc tgcaactgct gatgcaggag cgcagcgtga ccaaagcggc gaagcggatg
     4681 aacgtgaccc cttcggcggt gagtaagtcg ctggcaaagt taagagcgtg gtttgacgac
     4741 ccgctctttg tgaactcacc gctggggctg tcgcccacac cgctaatggt cagcatggag
     4801 caaaatctgg cggagtggat gcaaatgagc aacctgctgc tggataaacc gcatcaccag
     4861 acaccgagcg gcctaaagtt tgagctggcg gcagaatcac cgctaatgat gatcatgctt
     4921 aatgcgctgt cgaaacagat ctaccaacgt tacccgcagg cgaccatcaa attacgtaac
     4981 tgggattacg attccttaga tgccattact cgtggtgaag tggatatcgg tttttccggt
     5041 cgcgaaagcc atcctcgctc gcgggagctg ttaagctcgc taccgttagc cattgattat
     5101 gaagtgctgt ttagtgatgt gccttgcgtc tggttacgca aagatcatcc ggcactgcat
     5161 gaaacgtgga atctggacac ctttttacgt tatccgcata tcagcatttg ctgggaacag
     5221 agcgatacct gggcgctgga caatgtgtta caggagctgg ggcgcgaacg cacgattgct
     5281 atgagcctgc cggaattcga gcagtcgctg tttatggcag cgcagcccga caatctgcta
     5341 ctggcgaccg cgccgcgcta ctgtcagtac tacaatcaac tccaccaact gccgttggtt
     5401 gctcttcctc tcccgtttga cgaaagccag caaaaaaagc tggaagtccc ttttaccctg
     5461 ctgtggcaca aacggaacag ccataatccg aagatcgtct ggttacggga aaccattaaa
     5521 aatctttacg cgtcgatggc ataaccgaat cgtatgaaac gcgaccctat ttcacaataa
     5581 aatgtaaaaa atttgtaata aacttgtctg aatcgaactt ttagccgctt tagtctgtcc
     5641 atcattccag taaatgatta ctcttgtatt cataatggac cattaagcat ggagcgaaaa
     5701 atggcgactc actttgcccg agggatttta acggaaggac atctgatttc tgttcgtctc
     5761 ccctcccagt gtcatcaaga agcccgaaac attccccctc atcgtcaaag ccgttttctg
     5821 gcgtccagag gtttactcgc agaactgatg ttcatgctgt atggcattgg cgaattgccg
     5881 gaaatcgtca ccctgccgaa aggtaaaccg gttttcagcg ataaaaattt gccttcgttt
     5941 tctatttcct atgccgggaa tatggttggc gtggcgttaa caaccgaagg tgaatgtggc
     6001 ctcgatatgg aactgcagcg tgcgacacgc gggtttcata gcccacacgc gcccgataac
     6061 cacacctttt ccagcaatga atcgctatgg atcagtaaac aaaacgatcc taacgaagcg
     6121 cgggcgcagc tcatcacgct gcgccgaagc gtgctaaaac taaccggtga tgttttgaat
     6181 gacgatccgc gcgatctgca gctgctgccc attgccggac gcctgaaatg tgctcatgta
     6241 aatcatgtag aagcgttatg cgacgcggag gacgtgctgg tgtggtccgt ggcggtcacg
     6301 cccacgattg aaaagctcag tgtctgggag ttagatggca aacacggctg gaaaagcctg
     6361 ccggatattc acagccgcgc caacaatcct accagccgga tgatgcgttt tgcccagctc
     6421 tctaccatga aggctttttc gccaaattga tagacaacca caggagtcat tatgtctgaa
     6481 aaattgcagg tggttacgtt actggggagc ctgcgcaaag gctcatttaa tggcatggtt
     6541 gcacgtaccc tgccgaaaat tgctccggcg agcatggaag tcaatgcgtt accttccatt
     6601 gccgacattc ccctgtatga cgctgacgta caggaggaag aaggttttcc agcaacggtt
     6661 gaagctctgg cggaacagat ccgtcaggct gacggtgtgg tgatcgtcac gccggaatat
     6721 aactattcgg taccgggtgg gctgaaaaat gccatcgact ggctttcccg cctgccggat
     6781 caaccgctgg ccggtaaacc ggtgttgatt cagaccaact caatgggcgt gattggcggc
     6841 gcgcgctgtc agtatcacct gcgtcagatt ctggttttcc tcgatgcaat ggtgatgaac
     6901 aagccggaat ttatgggcgg cgtgattcag aacaaagttg atccgcaaac cggagaagtg
     6961 attgatcagg gtacgctgga ccacctgacc gggcaattga ccgcatttgg tgagtttatt
     7021 cagcgagtta agatctaaat aaaaaacccg ccagcaatca tgcatggcgg gtttttaatg
     7081 cgctatcgat tttaatgagc gtcgataaat acaatcttca ggataaacag cagcgcaacg
     7141 atgattacgc acgggctaag gtcacggaga cgcccggtac caatcttcat cacgcagtag
     7201 gagataaagc ccagcgcaat accttcggta atcgagaagc tgaacggcat catcacagcg
     7261 gtaataaacg ccggaacaga ttcagtaaga tcctgccagt tcacgcgagc aagacttgag
     7321 gtcatcaaca cgccaacgta aatcagcgcg ccagctgcag cgtagcctgg caccattccc
     7381 gccagcggcg acagaaagat aaccagcagg aacagcagac caacaaccac tgccgtcaga
     7441 ccggtacgac cgccaaccga tacgccggaa gaggactcaa tataagccgt aacggaagaa
     7501 gtaccgataa acgaaccggt cacggaagag atactgtcga catacagcgc ctgcttcatg
     7561 cgcgggaatt tccccttctc atctgccaga cctgctttat cggtcacgcc aatcaacgta
     7621 ccggaggagt caaacaggtt gaccagcatg aaagagaaaa tcacccctgc cagcccgagg
     7681 ttaaacgacc cggctaaatc tacatgaccc acgacagtca ttacgctcgg cggcgcagaa
     7741 acgatgccat tgtagtggac atcacccagc atccagccca gcagcgttgt caccacgata
     7801 gaaaccagca ccgctgcgtg aatgttgcgt gaggccagaa tagcaatgat gaagaagccg
     7861 aggatcccca gcagcacgct gtgagaagtc agattaccga tgctgaccag cgtttccggg
     7921 ttagcgacaa tcacacctgc gtttttcagc cccatcatgc caatgaacag accgataccg
     7981 ctggtaatgc ccacacgcag actcaccgga atgttggcaa tcatccagta gcgaacgcgg
     8041 aaaatcgtca gtaatagcag accaatcgcg ccccagaaga ttgcgcccat cccgacttgc
     8101 cacggcaagc ccatcgcctg cacaacgaca aaagcgaaga acgcattcag gcccatagcg
     8161 ggtgccagtg caactggcag gttagcaaac agccccatca taatactgcc gaatgcagca
     8221 atcagacagg tagtgacgaa gacggcgctg gtatccatgc cagcaacgcc aagaatttgc
     8281 gggttaacaa aaacgatgta aaccatcgtc aggaaggtgg taaaaccggc gatcacttcg
     8341 gtccgtgccg tcgtgccatg ttcgcgcagt ttaaacacgc gttccagcat cccctggcca
     8401 gaagcctggg ttgtgtgttg ttgactcatt atctatttcc gaacaaggag ggaaaatccg
     8461 tcgctatcgt ataccaaaat gagacaatat ggacggctga agataacttt ttttcattgt
     8521 atttgcttat acggcaacga ttgcgtcatg cgaattggca tgacgaaaac gttaaactaa
     8581 cgataaagga aagacatgtc ccagatagaa gcggtatttt tcgactgcga cggtacgctg
     8641 gtcgatagcg aggtcatttg ctctcgcgcc tatgtcacga tgttcaggga atttggcatt
     8701 aacgttgacc tggaggagat ttttacccgt tttaaaggcg taaaactcta cgaaatcatc
     8761 gacatcatca gtaaagaaca tggcgtgacg atggtccagg ccgatgccga gcacgtttac
     8821 cgcgcagaag tagcgcgtct gtttgatacc gaacttgcgg acatcgcagg cgcgaacgaa
     8881 ttgctggcga gcgttaacgt gccgatgtgc gtcgtctcta acggtccggt cagcaaaatg
     8941 cagcattcgc tgggtaagct caacatgctg cactacttcc cggaaaaact gttcagcggc
     9001 tacgatatcc agcgttggaa gcccgatccg gcattaatgt tccatgcggc aaaagcgatg
     9061 aatgtgaacg ttgaaaactg cattctggtc gatgactcca gcgctggcgc tcaatcaggg
     9121 atagatgccg gaatggaagt gttttacttc tgcgctgacc cgcacaataa gccgatcgtt
     9181 cacccgaaag tcaccacctt taccgagctg tcgcagctgc ctgcgctctg gaaggcacgc
     9241 ggctggaata taacgcgata atcgcctcct tacttcaacg cttacacatc agtcacgctg
     9301 gtttgagata aagaacacca ggtgtctgat gagtaagcgt ctattcccta tgaataaatt
     9361 atgtcgttat tcgtcctgtc atccaccctc tgcatagtta agccttggtt atatttttta
     9421 caatatcgga atactgttcg aaccaatcca gaatattacg catcgcgtca ctgatgtttt
     9481 tatcgtcttt atattgattc tcaagtctat tatttcgaat aatagtaagc ttagaacgta
     9541 ttttatcatc acaatcgaca attttcgcat tcaatgcatt tacttcatcc tgcact
//
LOCUS       DAZFKF010000152         9554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFKF010000152 DAZFKF010000000
VERSION     DAZFKF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.154"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..269
                     /locus_tag="ACHKA1_004742"
     CDS             <1..269
                     /locus_tag="ACHKA1_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFO5682292.1"
                     /translation="LALQYGFSVFPYTLDGQGDTAFPQALPAPPDVLKTFFPNIPVAT
                     PTTFLVNVNTLEALPLLQGATDAAGFMARMDTVLQMYGGKKGAK"
     gene            259..545
                     /gene="trbJ"
                     /locus_tag="ACHKA1_004743"
                     /pseudo
     CDS             259..545
                     /gene="trbJ"
                     /locus_tag="ACHKA1_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244900.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
     gene            526..918
                     /gene="trbF"
                     /locus_tag="ACHKA1_004744"
     CDS             526..918
                     /gene="trbF"
                     /locus_tag="ACHKA1_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFO5682293.1"
                     /translation="MKEKRNDAEQKNRKTKRDYDYERRVSDIYFDLFFVFVAAGTFLW
                     VIMHSIFDACIDSWKADPKLLNFHYMWNILMYVIPYTLWAFAGGFLIVYVRNPLNELI
                     NGGIRIFRLKRRMRRDNKLREGGNNASH"
     gene            905..2278
                     /gene="traH"
                     /locus_tag="ACHKA1_004745"
     CDS             905..2278
                     /gene="traH"
                     /locus_tag="ACHKA1_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFO5682294.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFATNTTQ
                     AGVWQGQAAGYAYGGSLYARNQVRNIQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAMGYFFDLALQTTVPEIKTAKDFLQKMANDINSMNLSSCQVAQGIVGG
                     LFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGF
                     ISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            2275..5118
                     /gene="traG"
                     /locus_tag="ACHKA1_004746"
     CDS             2275..5118
                     /gene="traG"
                     /locus_tag="ACHKA1_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO5682295.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVAGYEMVFAQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASSDPYTIIFSQPSPLRQVPNNNYSFLQSSEPFVSCK
                     DASVGLKDKLNFDTNTGGKTWNYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSAS
                     QIMRQNVTMNALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVLMRNLPM
                     VQTILVGITIGIFPLLVLAAVFNKLTLSVLKGYVFALMWFQTWPLLYAILNSAMTFYA
                     KQNGAPVVLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVRGLGAGFSSVYSHFASS
                     SITPTASAAGSVVDGNYSYGNMQTNNVNGFSWSTNSTTSFGQMTHQTGSGATATQTRD
                     GSMVMDASGAMSRLPVGINATRQIAAAQQEMARESMNKAESALHGFSSSIASAWNTLS
                     QFGSNRGSSDSVTSGADSTMSAQDSMMASRMRSAVESYAKAHNISDEQATRELASRST
                     RGSAGMYGDAHAEWGVKPKILGVGGGAGVRGGGRTSIDWNDEDAHQASSGSRASHDAR
                     HDIDARATQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTN
                     MTRSHEYAEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRR
                     AMAWSFVQEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQGIIEQRTQ
                     DSNIRNDVKQQVDNMVTEYRGNIGDTQNSIHGEENIVRGQYSELQNHHKTEALSQNNK
                     YNEEKSAQEGMPGADSPQELMNRAKEYQDKYKQ"
     gene            5134..5637
                     /locus_tag="ACHKA1_004747"
     CDS             5134..5637
                     /locus_tag="ACHKA1_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFO5682296.1"
                     /translation="MIMKNLAHVALITVIQLIACNLADWGGAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSKGVWYILMPVSSLFSPLLSLMTFIIGTLYELRRVCGCV
                     SVREWMQSQVNENTNEELHLDFDDVNFDYVDFYRTNPATGLAMHGGFDSAGNTFGTCW
                     QDYDDRR"
     gene            5669..6400
                     /gene="traT"
                     /locus_tag="ACHKA1_004748"
     CDS             5669..6400
                     /gene="traT"
                     /locus_tag="ACHKA1_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO5682297.1"
                     /translation="MKTKKLMMVTLVSSTLVLSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPSSERTVFLQIKNTSDKDMSGLQAKISDAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQTWLNSGYEGAIAGAALGTGITGYNSSSAGAALGVGIATGLIGMAADAMVE
                     DINYTMITDVQIAERTTSKVRTDNVAALRQGTSGAKVQTSTSTGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            6460..6720
                     /locus_tag="ACHKA1_004749"
     CDS             6460..6720
                     /locus_tag="ACHKA1_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682298.1"
                     /translation="MNRAESRLSQVLFLLGYDSEKYPEHVCRELLKELYRFADATGDS
                     ELAIACQRYLTALEPFSAYEIGVSATEYLPVSEYPTGRRRRK"
     gene            6888..7619
                     /locus_tag="ACHKA1_004750"
     CDS             6888..7619
                     /locus_tag="ACHKA1_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682299.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYSPVIQDNTATTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLVMYFINSLRHKELTEILTKISFPEKSGSNPLKPEEGRC
                     NLLSLCVLAPVFCTPKKLAEVSRQIIVTSAKEIAWTPGSSRIDGEQEENITWSLSSQT
                     HADKYDLINKTSRVSPDSGHFYEPGTALDYRERVRLFVQIQYSHESDWSEILRLTDIV
                     LEKVSSGRPAGGYHHRYTGYTWFVL"
     gene            7675..>9554
                     /gene="traD"
                     /locus_tag="ACHKA1_004751"
     CDS             7675..>9554
                     /gene="traD"
                     /locus_tag="ACHKA1_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO5682300.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDNILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDKKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLMAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKSAASLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PQ"
BASE COUNT         2452 a         2204 c         2543 g         2355 t
ORIGIN      
        1 aactggcgct gcagtacgga ttttccgtat ttccctatac cctggatggt cagggtgata
       61 cggcctttcc gcaggcgtta cctgcaccac cggatgtact gaaaacattc ttcccgaata
      121 tcccggtggc cacaccgacc accttcctgg tcaacgtcaa cacgcttgag gcattaccgc
      181 ttttacaggg ggcaacggat gccgccggtt ttatggcgcg gatggatact gttctgcaga
      241 tgtacggagg gaaaaaaggt gcgaaataag caggtggtgt tactcattgc cggaatatcc
      301 ggcgtcgcga cgggaataat cgtcagcctg aatatcccct ttatccgtca gggacttttt
      361 tatcccgcca gccctgcaga aattgtcgtt tcgctgtgtc ttaccttttc tgtttctgtt
      421 gtgtttttgt gggggcaatt gtgggatgga tttcagtatc tgaaatatat tacagccgca
      481 tgaacggtct gaatgaatca tcagaaatat cagaggaaac ttataatgaa agaaaaaaga
      541 aatgatgcgg aacaaaaaaa cagaaaaact aaacgggatt atgattatga acgcagggtt
      601 tctgatatat attttgatct gttttttgtg ttcgttgctg ccggaacttt tttatgggtg
      661 attatgcaca gtatttttga tgcctgtatt gattcgtgga aagcggaccc gaaattactt
      721 aatttccact atatgtggaa catcctgatg tatgtcattc cgtatacgtt atgggcgttt
      781 gcaggtggat ttcttatcgt atacgtcagg aaccctttaa atgaattaat aaacgggggg
      841 atcaggatat tccggctgaa acgccgtatg cgccgggata acaaattaag ggagggtggc
      901 aataatgctt cgcattaaac ctcttctggt tctttgtgcc gctttactga cggtcacacc
      961 tgcagcgtca gcggatgtga acagcgacat gaaccagttc ttcaacaagc tgggctttgc
     1021 caccaatacc acccaggccg gagtctggca ggggcaggct gccgggtatg cctatggcgg
     1081 ttctctgtat gcccgtaatc aggtcagaaa tatccagctt atctccatga cactgcccga
     1141 catcaatgca gggtgtggtg gcatcgatgc ctatctcggc tcattcagtt ttattaatgg
     1201 cgagcagctg cagcgttttg ttaagcagat tatgagtaac gcgatggggt acttttttga
     1261 ccttgccctg cagacaacgg tgcctgaaat caaaaccgcg aaagacttcc tgcagaagat
     1321 ggcgaatgac attaacagta tgaacctcag ctcctgtcag gtggcacagg ggattgtcgg
     1381 aggattattt cctcagactc aggtgtcaca gcagaaggtc tgtcaggata ttgcaggtga
     1441 gagcaatatt ttcgccgact gggccgcgtc ccgtcagggc tgcacggttg gggggcagtc
     1501 atcgtctgtt cgtgacaagg ccagtgacaa agataaggaa cgggtgctga agaacataaa
     1561 tatcatctgg gactcgctgt ccaaaaacag gatgcttgat ggtaacaaag agctgaaaga
     1621 gttcattatg acgctcacag gtacgctgat ttttggtgaa gacagcgaga ttacccccct
     1681 gccggcgcgt accactgacc gcgatatcct ccgggcgatt atggaggggg gaacggcaaa
     1741 aatttaccac tgcaacgatt cggacaagtg tctgaaggtg gtggcggatg caaatgtgac
     1801 catcgcccgg gataaagccc tgaaatcaca gattacaaaa ctgctcacca gtattcagaa
     1861 taaagccgtg agtgacactc cgctggatga cagggaaaaa ggcttcattt ccagcaccac
     1921 cgtccccgtc tttaaatatc ttattgaccc gcagatgctc ggtgtgtcca gcagcgtggt
     1981 ttaccagctg acggactata tcggctacga catcatgctc cagtacattc aggaactgct
     2041 tcagcaggcc cgggccatga ttgccaccgg gaactatcca cagaccgtgc tggacagtgt
     2101 gctggagaat ctgaatcagg cacagcaaca gattgcggtt ttccaggggc aggtaaaggt
     2161 ccagcaggat gcactgctgg ttgttgaccg tcagatgagc tacatgcgcc agcagctgtc
     2221 cgcccgtatg ctcagccgtt accagaacaa ctatcacttc ggagggagca cgctgtgaat
     2281 gaagtctatg tgattgccgg tggtgagtgg ttgcgcaata acctgaacgc cattgccgct
     2341 tttatgggga cccggacgtg ggattccatt gaaaaaattg cgctcacatt atctgttctc
     2401 gcagtggctg tgatgtgggt acaacggcac aacgtgatgg atttgctggg ctgggtggcc
     2461 gtgtttgtgc ttatcagcct gctggttaat gtccgcacat cggtgcagat tattgataac
     2521 agtgacctgg ttaaggttca ccgggtggac aatgtgccgg tcggtctggc gatgccactt
     2581 tcactgacga cccgtatcgg gcatgcgatg gtggccggct atgagatggt gttcgctcag
     2641 ccggacagcg ccacctacag caagacaggc atgctgttcg gggcgaacct gattgtgaaa
     2701 agcaccgatt tcctgtcccg gaatccggaa atcatcaatc tgtttcagga ctacgtccag
     2761 aactgcgtgc tgggtgatat ttacctgaac cataaataca cgctggaaga cctgatggcc
     2821 tcctctgacc cgtacaccat aatcttttct cagcccagcc ctttacgtca ggtgccgaat
     2881 aataattaca gttttctgca aagctcagag ccgtttgtgt cctgtaagga cgcatccgtc
     2941 ggactgaagg ataagctgaa ttttgatacg aataccggag gcaagacctg gaattattat
     3001 gtgcagcaga tatttggcgg caggccggac cctgacctgt tattcagaca actggtgagt
     3061 gacagttaca gttatttcta cggctccagc cagtctgcca gccagattat gcgccagaac
     3121 gtcaccatga atgccctgaa agagggtatc accagtaatg cggcccgtaa cggtgatacc
     3181 gccagcctgg tgaatctggc caccacctca tcgatggaga aacagcgtct ggcacatgtc
     3241 tccattggtc atgtgctgat gcgaaacctg ccgatggtac agaccattct ggtgggcatt
     3301 actattggta tcttcccgct cctggtgctg gctgcggtct tcaacaaact gacgctgtcc
     3361 gtcctgaaag gctatgtgtt tgccctgatg tggttccaga cctggccgtt gctgtatgcc
     3421 atcctgaaca gtgccatgac attctatgcg aagcagaacg gggctccggt cgtgctctct
     3481 gaactctctc agatacagct gaaatactcc gacctggcat caaccgcagg ttatctttca
     3541 gcaatgatcc cccctctgtc atggatgatg gtacgaggcc ttggtgccgg attttccagc
     3601 gtatacagcc actttgcgtc ttcttccatc acgccaaccg ccagtgcggc aggcagtgtg
     3661 gttgacggta attactccta cggcaacatg cagacgaata acgtgaacgg cttcagctgg
     3721 agcaccaaca gcaccacgtc atttggtcag atgacgcacc agaccggtag tggtgcaacc
     3781 gccacgcaga cccgtgacgg cagtatggtg atggacgcca gtggggcaat gtcccgtttg
     3841 ccggttggta ttaatgcaac gcgtcagatt gcggcggcac aacaggaaat ggcccgggag
     3901 tcgatgaata aggcggaaag tgccctgcat ggtttcagca gcagtattgc cagtgcctgg
     3961 aacacactca gccagtttgg ctctaaccgg ggaagcagtg attcagtcac cagtggcgct
     4021 gacagcacga tgagcgcgca ggactccatg atggccagcc gtatgcgcag tgcggtggaa
     4081 agctatgcga aggcgcataa tatcagcgat gagcaggcga caagagaact ggcatccaga
     4141 agtacgagag gttctgcggg gatgtatggt gatgctcatg ctgaatgggg ggttaaacct
     4201 aaaattcttg gtgttggtgg tggcgcaggt gtcagaggtg gtggtaggac aagtattgac
     4261 tggaatgatg aggatgcgca tcaggccagc agtggttcac gggccagcca tgatgcccgt
     4321 catgatattg atgccagagc tacacaagac tttaaagaag caagcgatta ctttaccagt
     4381 cgcaaggtca gtgaatccgg cagccatact gacaataatg ccgattccag ggtggaccag
     4441 ctttctgcag ccctgaactc agcgaaacag agttacgacc agtacacgac gaatatgacc
     4501 cgcagccatg aatatgctga aatggcctcc cgtactgaaa gcatgagtgg gcagatgagt
     4561 gaagacctgt cacaacagtt tgcacagtat gtgatgaaac acgcgccgca ggatgcagaa
     4621 gctattctga caaacaccag ttcaccggag attgcagaac gtcgtcgggc aatggcctgg
     4681 tcttttgttc aggaacaggt acagcctggt gttgataacg cctggcgtga atcccgtggc
     4741 gatatcggta aaggaatgga gagcgtacct tcgggcggtg gcagccagga tattattgca
     4801 gaccatcagg ggcatcaggg tattattgag caaagaacgc aggacagtaa tatccgtaac
     4861 gatgtgaagc agcaggttga taatatggtc acagaatatc ggggcaatat cggggatact
     4921 cagaacagca tccacggcga ggaaaatatt gtaagagggc agtattctga actgcaaaat
     4981 caccataaaa cagaggcatt atctcagaat aataaatata atgaagaaaa atcagctcag
     5041 gaaggaatgc ccggtgctga cagcccacag gaacttatga acagggcaaa agagtatcag
     5101 gataagtata agcaataatg ctgggtttgg gagatgataa tgaaaaattt agctcatgtt
     5161 gctctgataa ctgtgataca gctcattgcc tgtaatctgg ctgattgggg gggcgctgag
     5221 acagttttta tcctgtgctt tattgtacta tggcagggat tgtttatatg gttgttttct
     5281 caaatacgaa aaaaacggca tgtgtcggat gagtttaaat tcagtaaagg cgtttggtat
     5341 atactgatgc cagtatcttc tttattctct ccgttattaa gcctgatgac ttttatcatt
     5401 ggtacactat acgaattaag gcgagtttgt ggttgtgtaa gtgtaagaga atggatgcaa
     5461 tctcaggtaa atgaaaacac caatgaagaa ttgcatctgg attttgatga tgtgaatttt
     5521 gattatgtgg atttttatcg tactaatccg gcaacaggat tagctatgca tggaggattt
     5581 gattcagcag gaaatacttt tgggacttgt tggcaggact atgatgatag gcgataagaa
     5641 aactgtagct tgtaataaag gtaatttgat gaaaacaaaa aaattgatga tggttaccct
     5701 ggtcagttcc actctggtcc tttcagggtg tggtgcgatg agcacagcaa tcaagaagcg
     5761 taatcttgaa gtcaaaactc agatgagtga aaccatctgg ctggagcctt ccagtgaacg
     5821 caccgttttc ctgcaaatca aaaacacatc tgacaaggat atgagtggat tgcaggcaaa
     5881 aatcagtgat gccgttaaag ccaaaggcta ccaggttgtg acctcacctg acaaggcata
     5941 ctactggatc caggccaatg tcctgaaggc agacaaaatg gatttgcggg agtcccagac
     6001 ctggctgaac agcggttatg aaggtgccat tgccggtgca gcattgggca ccggcatcac
     6061 aggatataac tccagctctg ccggtgcggc gctgggtgta gggattgcca cagggttgat
     6121 tggtatggct gccgatgcaa tggtggagga tatcaactac accatgatca cggatgtgca
     6181 gattgcagag cggacaacat ctaaagtcag aactgataat gttgctgctc tccgccaggg
     6241 aacctccggt gcaaaagtgc agaccagcac atcaaccgga aatcaacata aataccagac
     6301 ccgtgtggta tctaatgcca acaaggttaa cctgaaattt gaagaagcga agcctgtact
     6361 ggaagaccag ctggctaaat ccatcgcgaa catcctgtaa attacccatc cggcccggac
     6421 agattgtacc gggccgatat tcctgccccg gatgaataca tgaaccgtgc agaatctcgt
     6481 ctctcgcagg tactctttct gctgggctat gacagtgaaa agtatcctga gcatgtatgt
     6541 cgggagttat taaaagaact ttaccggttt gctgatgcaa ccggagattc tgaactggcc
     6601 atagcctgcc agagatacct gacagcactg gaaccgttct ctgcatatga gatcggcgta
     6661 agcgcaacag aatatcttcc tgtgagtgaa tatccgaccg gacgtcgcag aagaaaatag
     6721 ctgacgcctc gcgttgctcg ttgtccaacc ccggaaagta acaaaataaa ttttgtcact
     6781 tcccggcgtt tccgaaataa atatgagaac actgaacggt tgtgcaaatt ctttgtgcga
     6841 ccgtgaagtg ttgccggcga agccggaagc ttccggagaa agtcattatg acatcagaca
     6901 atatcgctga atatgttggt gagaataatc aatttactga gaagcctgta actgaatact
     6961 ccccggtgat acaggataat acagcgacca caaaagatta tctgtacagc aataatattg
     7021 atactgacct tttagtggtt atcggagggc ttattgttat attgtttctc atgatgcttg
     7081 tcatgtactt tattaattct ctcaggcata aggaattaac tgaaatactg acaaaaatat
     7141 cttttcctga gaaatccggg agcaatccgc ttaagccgga agagggccgc tgtaatttgt
     7201 tgtccctgtg tgtgctggcc ccggtattct gtacccccaa aaaactggca gaagtctcca
     7261 gacagatcat cgtgaccagt gcgaaggaaa ttgcctggac gcccgggagt agccgtattg
     7321 atggtgagca ggaagaaaat attacctggt ctctgtcgtc acagactcat gccgataaat
     7381 acgatctcat caataaaaca tcccgtgttt ctcctgacag tggccacttt tatgaaccgg
     7441 gcacggctct ggattatcgt gagcgggtac gcctttttgt tcagatccag tattcacacg
     7501 agagtgactg gtctgaaata ttgcgcctga ctgatatcgt gctggaaaaa gtttcttccg
     7561 gcagaccagc aggcggctat caccaccggt atacagggta tacatggttt gttctgtgaa
     7621 tgtattccac accgctcttt tctgctctgt acctgacttt accggagttt catcatgagt
     7681 tttaacgcaa aggatatgac ccagggcggt cagattgcgt ccatgcgtat ccgcatgttc
     7741 agccagatcg ccaatatcat gctttactgc ctgtttattt ttttctggat cctcgttggt
     7801 ctggttttat gggtaaaaat aagctggcag acgtttgtga acggctgtat ttactggtgg
     7861 tgcaccacgc tggaaggcat gcgggattta atcaggtccc agccggtcta tgagatccag
     7921 tattacggca aaaccttccg gatgaacgcg gctcaggtac tgcatgataa atacatgatc
     7981 tggtgcggtg agcagctatg gtctgcattc gttctggcgt ctgtcgtggc cctggttatt
     8041 tgcctgatca ccttctttgt tgtctcctgg attctggggc gtcagggtaa acagcagagt
     8101 gaaaatgaag tcacaggtgg tcgtcagctg actgacaatc cgaaagaggt tgccaggatg
     8161 ctgaaaaaag acggcaagga ctccgatatc aggattggcg atctgccgat tatccgggat
     8221 tctgaaatcc agaatttctg tctgcacggc acggtcgggg ccggtaagtc ggaagttatc
     8281 cgtcgtctgg ccaactacgc ccgtcagcgc ggtgatatgg tggtgattta tgaccgatca
     8341 ggggaatttg ttaaaagtta ctatgacccg tccatcgata atatcctgaa tccgctggat
     8401 gcccgctgtg ccgcctggga tttgtggaag gagtgcctga cacagccgga ttttgataat
     8461 acggcaaata ccctgatccc gatgggcaca aaagaagacc cgttctggca gggctcagga
     8521 cgcaccattt ttgcggaagc ggcgtacctg atgcgtaatg accccaaccg cagctacagc
     8581 aaactggtgg atacactact ctccatcaaa attgaaaaac tgcgtacctt cctgcgtaac
     8641 tcaccggcgg ccaacctggt ggaagagaaa atcgagaaaa cggcgatttc catccgtgct
     8701 gtgctgacca actacgtgaa agccatccgt tacctgcagg ggattgagca taatggtgag
     8761 tccttcacca tccgtgactg gatgcggggt gtccgtgaag ataagaaaaa cggctggctg
     8821 tttatttcgt cgaatgccga cacccatgct tccctgaaac cggtgatctc catgtggctg
     8881 tccatcgcca ttcgtggcct gatggcgatg ggggagaacc gtaaccgtcg tgtgtggttc
     8941 ttctgtgatg agttacccac gttacacaaa ctgccggacc tggtggagat cctgccggaa
     9001 gcccgtaagt tcggtggctg ttatgtgttc ggtatccagt cctatgccca gctggaagat
     9061 atctacggtg agaaatcggc agcctcgctg tttgacgtca tgaacacccg tgcctttttc
     9121 cgttccccca gccataagat tgcagagttc gctgccggtg aaattggtga gaaagagcac
     9181 ctgaaagcca gcgagcagta ttcctacggt gctgatccgg tacgtgacgg ggtatcgacc
     9241 ggtaaggata tggagcgcca gacgctggtc agttattccg acattcagtc tctgccggat
     9301 ctgacctgtt atgtcaccct gcccggaccg tatccggcag taaaactctc cctgaaatat
     9361 caggcacgac cgaaggttgc tccggagttt attccgcgtg acatcaatcc tgaaatggag
     9421 aaccgtctga gtgccgtact tgccgcaagg gaagcagaag gtcgtcagat ggccagcctc
     9481 ttcgaaccgg atgtcccgga ggttgtttcc ggagaagacg tgactcaggc tgaacagccg
     9541 caacagccgc aaca
//
LOCUS       DAZFKF010000153         9546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFKF010000153 DAZFKF010000000
VERSION     DAZFKF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.155"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..107
                     /locus_tag="ACHKA1_004752"
     CDS             <1..107
                     /locus_tag="ACHKA1_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO5682301.1"
                     /translation="ISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
     gene            complement(91..729)
                     /gene="ytfB"
                     /locus_tag="ACHKA1_004753"
     CDS             complement(91..729)
                     /gene="ytfB"
                     /locus_tag="ACHKA1_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO5682302.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            948..1568
                     /gene="fklB"
                     /locus_tag="ACHKA1_004754"
     CDS             948..1568
                     /gene="fklB"
                     /locus_tag="ACHKA1_004754"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5682303.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            1877..3289
                     /gene="cycA"
                     /locus_tag="ACHKA1_004755"
                     /gene_synonym="dagA"
     CDS             1877..3289
                     /gene="cycA"
                     /locus_tag="ACHKA1_004755"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO5682304.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3334..3996)
                     /gene="ytfE"
                     /locus_tag="ACHKA1_004756"
     CDS             complement(3334..3996)
                     /gene="ytfE"
                     /locus_tag="ACHKA1_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO5682305.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHNTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4104..5069)
                     /gene="ytfF"
                     /locus_tag="ACHKA1_004757"
     CDS             complement(4104..5069)
                     /gene="ytfF"
                     /locus_tag="ACHKA1_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5682306.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGPLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5177..6037)
                     /gene="qorB"
                     /locus_tag="ACHKA1_004758"
     CDS             complement(5177..6037)
                     /gene="qorB"
                     /locus_tag="ACHKA1_004758"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO5682307.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHRFNVNN"
     gene            6126..6506
                     /gene="ytfH"
                     /locus_tag="ACHKA1_004759"
     CDS             6126..6506
                     /gene="ytfH"
                     /locus_tag="ACHKA1_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO5682308.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6624..8567)
                     /gene="cpdB"
                     /locus_tag="ACHKA1_004760"
     CDS             complement(6624..8567)
                     /gene="cpdB"
                     /locus_tag="ACHKA1_004760"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO5682309.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8757..9497
                     /gene="cysQ"
                     /locus_tag="ACHKA1_004761"
     CDS             8757..9497
                     /gene="cysQ"
                     /locus_tag="ACHKA1_004761"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO5682310.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2170 a         2444 c         2515 g         2417 t
ORIGIN      
        1 acatcagtat ggtttcaccc caggcactgg aagccagacg taaggtcatc aaagagacaa
       61 tccttagctg gcttctggtt gacccttcat ctaccgcgcg cgaataaaac tgccatccgg
      121 ctggcgggta aacaggacct gttgattatt ccccgtatca atggttaagc ctgtcaccac
      181 gccgctggcg ttctggcgga ttttcaccat ctgcccattt tgcaaattac tcagcggttt
      241 acccgcgcct tctacctgcg ccattgcata gacatcggta gcgggcagtc cgtggtcgcg
      301 gaacagctgc gccatcgttt taccgggttc gacacggtaa gagcgccatt gattatcaat
      361 tccgctatct ggctggaatg gttgtgtttg ggtagtttgc ggctgctctt ctggctggcc
      421 ttcctgtata ggttctggag cgaccggagc cacctgatca ggatcatttt gtggtgtgac
      481 taattgcgcc cgcagctgtt cttctgtcgg cggttgtgat tgtgactgaa tatctaactg
      541 cgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca gatggaagca gaaatccgac
      601 caccaataca atggcggcaa taatgatccc ccgacgatgc atcggcggca gcgggtccat
      661 gaagcgaaaa ttgtccggcg cgtgccagac tttctccagg gttggtttta gttcaaagcg
      721 cccgggcatg gtttcctcct gctccgcgtc ttgttcctca atcatagcct atgaataagc
      781 taacgctatg atgtccgtgg taaacccgcc tttattatat tcatacggga tattgctatt
      841 gtttcttttt ccccgggatt tgtcatcatt cctgcgacaa agttttaccc aaagaagtgt
      901 ggctgatatg ctgcccgcta cttttatacc ctaagaaagg aaatacgatg accaccccaa
      961 cttttgacac catcgaagcg caagcaagct acggcattgg tttgcaggta gggcaacaac
     1021 tgagtgaatc tggcctggaa gggctgctgc cagaagcact ggttgcaggt attgccgatg
     1081 cgctggaagg caaacatccg gctgttccgg ttgatgtggt gcatcgcgca ctgcgtgaaa
     1141 tccacgaacg cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg
     1201 tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc
     1261 aattccgcgt gatcaaccag ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg
     1321 ttcattacac cggtaaactg atcgacggca ccgtgtttga cagctccgtt gctcgtggtg
     1381 aacccgctga attcccggtt aatggcgtga tccctggctg gattgaagca ctgactctga
     1441 tgccggtagg ttctaaatgg gaactgacta tcccgcagga actggcatat ggcgagcgtg
     1501 gcgcaggcgc gtccatccct ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa
     1561 tcctctaagc agcgcattct gttcccctcg agccagaggg gagcaggcat tcagcaataa
     1621 ccccttcagt ttgccaaacg gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt
     1681 ttgtgagctg tttcgcgtta tcaccgtgat atgacactca ctttaaacat aaaattaaca
     1741 ttagatctaa atcttagtat tcatcccgcg tattgttacc taatatcgat gagtcccgat
     1801 acagattcgt cgtatcatag actgactaaa ggccgtagag cctgaacaac acagacaggt
     1861 acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc aggctccggc
     1921 tgaacagtcg ctacggcgca atctcacaaa ccgacatatt cagcttattg ccattggcgg
     1981 tgccattggt acggggttgt ttatggggtc tggcaaaacg attagccttg ccgggccgtc
     2041 gatcattttc gtttatatga tcattggttt tatgctcttt ttcgtgatgc gggcaatggg
     2101 ggaattgctg ctttcgaatc tggaatacaa atcttttagt gacttcgctt ccgatttact
     2161 cgggccgtgg gcaggatatt tcaccggctg gacttactgg ttctgctggg ttgtaaccgg
     2221 tatggcagac gtggtggcga tcacggctta tgctcagttc tggttccccg atctctccga
     2281 ctgggtcgcc tcgctggcgg tgatagtgct gctgctgacg cttaacctcg ccaccgtgaa
     2341 aatgttcggt gagatggagt tctggtttgc gatgatcaaa atcgtcgcca ttgtgtcgct
     2401 gattgtcgtc ggcctggtca tggtggcgat gcactttcag tcaccgactg gtgtggaagc
     2461 gtcattcgcg catttgtgga atgacggcgg ctggttcccg aaaggtttaa gtggcttctt
     2521 tgccggattc cagatagcgg ttttcgcttt cgtggggatt gagctggtag gtacaacagc
     2581 tgcggaaacc aaagatccag agaaatcact gccacgcgcg attaactcca ttccgatccg
     2641 tatcattatg ttctacgtct tcgcgctgat tgtgattatg tccgtgacgc cgtggagttc
     2701 ggtagtcccg gagaaaagcc cgtttgttga actgtttgtg ttggtaggtt tgcctgctgc
     2761 cgcaagcgtg atcaactttg tggtgctgac ctctgcggcg tcttccgcta acagcggcgt
     2821 cttctctacc agccgtatgc tgtttggtct ggcgcaggaa ggtgtggcac cgaaagcgtt
     2881 cgctaaactt tctaagcgcg cagtaccagc gaaagggctg acgttctcgt gtatctgtct
     2941 gctcggcggc gtggtgatgc tgtatgtgaa tcccagcgtg attggcgcgt tcacgatgat
     3001 cacgaccgtt tccgcgattc tgtttatgtt cgtctggacg attatccttt gctcgtacct
     3061 tgtgtatcgc aaacagcgtc ctcatctaca tgagaagtcg atctacaaga tgccgctcgg
     3121 caagctgatg tgctgggtat gtatggcgtt ctttgtgttc gtggtcgtgt tgctgacact
     3181 ggaagatgac actcgccagg cgctgttggt caccccgctg tggtttatcg cgctggggct
     3241 gggctggttg tttattggta agaagcgggc tgctgaactg cggaaataac cgcattatca
     3301 tgctggatgg cgcaatgcca tccagctttt agatcactca cccgccagcg cgcgtgggaa
     3361 cagcacatta ttttccaggc tgatgtgatc catcaggtca tcaatcagtt cattaatgcc
     3421 gttatacatc gctttccagg tggtgcaggc ttccggcggc ggtgtgacgt tattggtatt
     3481 gtgtttgatc acttccagca gttcacccgc ttcatcgtgc tcgctttcca ttacgctgat
     3541 tggccccatt gcctggctgc ccatgccttg tttgatcatc gggaagagga tctgctcttc
     3601 tttcatcatg tggctggaaa gctcttcatg cagcatggtc aggtattttg tcagcccttt
     3661 tggcacgctc ggtttgtcgg cgtgaacgcg ctcgacttta gtcgcttgca gaatcagctc
     3721 cggcagttgc tcgcggtgac gatcgtggta gcgcacgatg atatggtcga tgatttctgc
     3781 cagcggggcg ctacgccagt ctttctcaat cggttgttca gcaagctttg ccagctcagc
     3841 ttcaatgacc tcaacatcca gttctttacg tgccgccgca cgcgccagcg tttgcttacc
     3901 gccgcagcag taatccatat catatttacg aaacagagcc gaagcgcgag gaatagagag
     3961 cgccagttca cctaaaggtt ggtcgcgata agccatagct gatacctcat tcttaataag
     4021 ataagatgta ttttaaatgc atctttaagg caaaagctat aacccttacg tagcagaggc
     4081 ttatttaact cactgccagc gtgtcaactt tctgagacag attcagttaa aggtttttct
     4141 ggttttgctc tgaccgcgat gaccacgcca atcactaaca gcgcgatacc gctcagcgtc
     4201 attagcggcg gcatttgctg gcggagtaaa aaggtgtaca gcaaacctgc cagcgtttcg
     4261 aaaacaatca gcggcccgag aatcacggtc ggtaatcgct ggctggcgac gttccagcag
     4321 agtgcgccga cccatgagca aagcacggct atcgcaatca tcagactaat aaacaccagc
     4381 ggacgggggc caaagggtag ggagaagtcc ggcgtttgcg tattcagcca gtaacaagcg
     4441 acgagatagc cgatgagaga aaccggcagt gtgaccagcg cctgcgccgt tgcccacatc
     4501 atcggatgtt tgtcgggatt ttcccgcagc cagcgggcgt tgcgcagggc ataccacgcc
     4561 cagcagacca cggaaactaa cgccagcacg atgccagagg tataacgtgc ccagtcaaaa
     4621 tcggggagtc cgtggtttaa ctcagcaata ttcacacacg ccaggccgat gccaatacaa
     4681 atcagtgccg gggcgagttt tccccacgcg agtttgccgt cgcgctggct ataaagcaga
     4741 ttggcaaaga caggaatgac caccggcagg gggccgataa tcatcgtgga aacaggcgcg
     4801 ccagtacgtt gaatggcact ggcaaggcag aagtaataaa tgaggttgcc catcatagtg
     4861 agcatcaagg cggtaagcca gtcgcgacgc gccaactgac gcagacgcac gcgtcccagc
     4921 caggcaatgg gcagcgcaat taaccctaac gccagataac gccccatcga ctgcaacatc
     4981 gccgggtatt ccggcacgat caacggcccg acaaaaataa gcccccacat caaccctgct
     5041 aacagggcgt acagcacgcc gctaatcatt actggcatcc attgatctgt cagaagaaag
     5101 ttcaggaagc agacatgatt gctcccggca tcccgtcatt atcagagagg atgcgggagg
     5161 atgccacttt aattaactag ttattaacat taaaacgatg gcttacgctt tcggctaacg
     5221 ttgtcgttgg gcggccaatc aatttgctaa gcgttttgct gtcatcaaat agaccgcctt
     5281 tcgatgcgcc aacgtcagaa tccgccagca tatccgccag tccgtcgggc agtccgacgc
     5341 ttttcagtgc cgcggcgaaa tcggcttcgc tcagattttg ataggtaacc tgtttgccgc
     5401 tctgtttggt cagctctgcc gctaactgtg tcaacgtcca ggcactatcg cccgccagtt
     5461 cgtaaacctt gccttcgtga ccggcttcgc taatcacgcg tgccgcagct gccgcataat
     5521 ctgcccgcgt tgctgaggcg attttgccat cgcccgccgc accgataaat acgccgtgtt
     5581 ccagtgctgc cggggcgctg gcgaggtagt tttcgctgta ccagccgttg cgcagcaggg
     5641 tgtaaacgat gccagaatca gccaacattt tctccgtctc aatgtgctca tcggcgaggc
     5701 cgagcgggga ggtatctgca tgtagcaggc tggtataagc gataaatttc acgccagccg
     5761 cctttgcggc attaataaca ttacgatgct gcggggcacg ttgacccact tcgctggaag
     5821 agatcagcag tagtttttcc actccctgaa gtgcagatgt cagtgcggct tcatcgccgt
     5881 agtcagcctg acgcacggta atgccttgtg ctgccagggc ctgggctttt gccggattac
     5941 gaacgatagc cactatttgg ctggcaggaa ccgttttcat caaagattca ataacatagt
     6001 gaccaagttg gccagtggca ccagtaatag cgatcatggg aagtctccat cgttttgtct
     6061 tgagtttaaa tacgctaaca cctaaactta cttttagtaa gtacgtacaa aaaggtaagt
     6121 atgaaatgag tcaggttagc ctgtcgcaac aactgaaaga gggtaatctc tttgcggaac
     6181 agtgcccgtc gcgcgaggtg ttgaaacacg tcaccagccg ttggggggtg ttgattctgg
     6241 tggcgctacg cgaaggtact catcgcttta gcgacctgcg gcgcaaaatg ggcggggtga
     6301 gtgaaaagat gcttgcgcag tcgttacagg cgctagagca ggatggtttt cttaaccgta
     6361 tcgcgtatcc ggtggtgccg ccgcatgtgg aatatagcct cacgccgctg ggcgagcagg
     6421 tgagcgaaaa ggttgctgca ctggcggact ggattgagtt gaatttgccc gaggtgttag
     6481 cggtgcggga tgaacgtgcg gcataactgc cggatgcgcg gcctacggtg tatgacttgt
     6541 agggcggata agatgcgcca gcatcgcatc cgccaataaa tgccggatgc gattgatgat
     6601 ttataggccg aaaaagaagt gttttactta ctcaaatcca cctgataaat cgcaaacccg
     6661 atatcatcgg tcgcgacttt attcatcgga tactgccctt tctctttaat aaacgctgcg
     6721 gctttatctg acggggaagt ttcgaaacgg atatccagtt tcttatcgcc agctatcggt
     6781 gctaaacgcc agttgttatc tgccgccggg tgaatttccc ccgcacgttt cgactcatca
     6841 gcaatccacg ctgccagcac cgagcggttc tcatccggtg aagcaaaagc gatatggctg
     6901 tcgcccgtac cggcaaattt gccgccgtaa gcgcgatagt tattggtggc aacgaggaac
     6961 atagcgttcg gatcaatcgg cttgccatta aaggtcaggt tcttaatcct ttccgcattg
     7021 gcattaatca tctggcactc gccgtcataa cgggcgggct gggtaacatc aatctgataa
     7081 ttcacaccat caataacatc aaagttatag gtgcggaaac cgtcccagtt gatgagtgac
     7141 tgtggtttcg tgctgttggg atcaatctgg ttaaactgtc cggcggagca ctccagccac
     7201 tctttcacct ctttaccgct ggctttcacc acaatcagcg tattgggata gagataaaga
     7261 tcggcggcat tacggaaagt cagctgacct ttttccacct ctacatagct tgccgggtca
     7321 tttttgcgac caccaacttt aaacggtgcg gcagctgaaa gcaccggcag ttttgccaga
     7381 tccggatcgc cctgaatgta atgctcgaca tacgcttttt gcgcgttgtt caccacctgc
     7441 acggtcggat cgtcctgcac cagcgccaga tagctataca tattgtcggc agatttaccg
     7501 attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg ctttgagtgt ttctaccagc
     7561 ttgctgtctt ccgccgcgag ggatttttta ttggcgatgt cgtaaatcgg ccgtgcttcc
     7621 gctttcgcct gcgtcacctg ccatttaccg ctgtcattac tgagttgtaa gtcgaccacc
     7681 ccaagatgat cgccccacat gcccggcatt accgccggaa caccattcag cgtgcctttg
     7741 gcgatatcag ccccttcgat atcagcaaaa tctttacccg ggaaaacggc gtgagcatgg
     7801 ccaaacataa tggcgttaac gcccggaatt tcactgaggt aataaactga gttttccgcc
     7861 atcactttat acggatcggc agatagcccg gaatgcgcca gaacgacaac gacatcggca
     7921 cctttctcgc gcatttcagg cacgtatttg cgcacggttt cggtaatatc attcaccgtc
     7981 actttcccgg ataaattagc tttatcccag cccatgattt gtggcggcac gacgccaata
     8041 tagccaatct tcagcgtctg tttttttccg tctttatcga ccacttcggt gtctttaatt
     8101 aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa tgacgttggc atttacataa
     8161 gggaatttcg ctcctgccag cgcatttttc aggtaatcca gaccgtagtt aaactcgtgg
     8221 ttgccaagcg ttccgacggt atagtccagc gtatttaatg ccttatagac cgggtgaata
     8281 tcgcctgctt ttaatccttt cgccgacatg taatctgcca gcggactccc ctgaatcaag
     8341 tcgccgttat caaccagtac gctgtttttc acttcattgc gggcatcgtt aatcaggctt
     8401 gccgtacgta ccagtccgaa tttttccgtg gcggtgtctt tgtaataatc gaaatccatc
     8461 atgttgctat gcagatcagt ggtttccatg atacgcagat cgaccgtcgc tgcattcaca
     8521 ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact taatcatcag ggacatcctt
     8581 ttatcatcgg gaatacgaaa gaaaagggag aataaacgcc ttacttatag aacagtgaag
     8641 aatgccacaa ttttacgctt tgaaaatgat gacactatca cagttggcgc attcattaac
     8701 gatagggtat aagtaaaaca ataagttaac accgctcaca gagacgaggt ggagaaatgt
     8761 tagatcaagt atgccagctt gcacggaatg caggcgatgc cattatgcag gtctacgacg
     8821 ggacgaaacc gatggacgtc gtcagcaaag cggacaattc tccggtaacg gcagcggata
     8881 ttgccgcaca caccgttatc atggacggtt tacgtacgct gacaccggat attccggtcc
     8941 tttctgaaga agatcctccc ggttgggaag tccgtcagca ctggcagcgt tactggctgg
     9001 tagacccact ggatggtact aaagagttta ttaaacgtaa tggcgaattc accgttaaca
     9061 ttgcgctcat tgatcatggc aaaccgattt taggtgtggt gtatgcgccg gtaatgaacg
     9121 taatgtacag cgcggcagaa ggcaaggcct ggaaagaaga gtgcggtgtg cgcaagcaga
     9181 ttcaggtccg cgatgcgcgc ccgccgctgg tggttatcag ccgttcccat gcagatgcgg
     9241 agctgaaaga gtatctgcaa cagcttggcg aacatcagac cacgtccatc ggctcttcgc
     9301 tgaaattctg cctggtggcg gaaggacagg cgcagctgta cccgcgcttc ggaccaacga
     9361 atatttggga caccgccgct ggacatgctg tggctgcagc tgccggagcg cacgttcacg
     9421 actggcaggg taaaccgctg gattacactc cgcgcgagtc gttcctgaat ccggggttca
     9481 gagtgtctat ttactaaatt cagatggcag aaacagtgta tttcctgatt ctgccatcct
     9541 gattta
//
LOCUS       DAZFKF010000154         9441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFKF010000154 DAZFKF010000000
VERSION     DAZFKF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.156"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            98..3037
                     /locus_tag="ACHKA1_004762"
     CDS             98..3037
                     /locus_tag="ACHKA1_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5682311.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDLTAPGTLESLITALAERMNTAY
                     KNIGHKISFVFERDPERGRNEIEVMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRIRKLAEHAPPARFAQDPWRWTLSALKIRHDAFLD
                     TLEQALTHDSDGLLVRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGED
                     TSVFHAPSLNLQLFTTQPETHGSLIKAGDLWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPGGMKALGVKKTLLTYSSFISPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHGSGPVPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEQRKDEAVGIILSNDPEHTRNLFDVMYGAKKPATPEKNFMVSVLCALCVD
                     TGTGQPCNPGDTRQIISNLVDLAFREYGENNPRLYRAGTEPLVDLALEESGIAEQHDA
                     GWWNAATWFEIRDMLHMAGNIPAAQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDN
                     SAELLLDYIRRALDQGHSDYPMMSGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTG
                     IMYLLAGQIAGGDFVLPQYQEEIRKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAK
                     GINFIWEALDTQELEQRKFGIRTVLSTQLLQDYPPAVLKSANTLWLLRYRPDEIPFLR
                     DNFGVPEVTLRRFLKMPEGAAPDGSGVPVLAVFRVKNGTLARILKFTLGPLELWALNS
                     SPKDSALRRALTQELGSLRARQILAENFPRGSATSLIEHRARTHDSENVIHDLAAELI
                     RKQGYHL"
     gene            3037..3654
                     /locus_tag="ACHKA1_004763"
     CDS             3037..3654
                     /locus_tag="ACHKA1_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFO5682312.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGVRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     SPLTALMSDNTWLWQDSPGTPALTAGDWPVMETDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESHSHRVPDILYPLKTEKKECKDNQQPHCLHS"
     gene            3615..4820
                     /gene="traW"
                     /locus_tag="ACHKA1_004764"
     CDS             3615..4820
                     /gene="traW"
                     /locus_tag="ACHKA1_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFO5682313.1"
                     /translation="MQRQPATTLLALLIGCAVIPSTHAYSVTVVASQPVTTQIMPELA
                     AIQGVMAEMVRMQTATGTAINQSSEKLASVIAQDGQATRQQMIFSNETRRLEEARKSF
                     SVPDSICSESASGVAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPIREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGELPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            4817..5386
                     /gene="traX"
                     /locus_tag="ACHKA1_004765"
     CDS             4817..5386
                     /gene="traX"
                     /locus_tag="ACHKA1_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFO5682314.1"
                     /translation="MKDDNSTDKKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     GQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            5459..7618
                     /locus_tag="ACHKA1_004766"
     CDS             5459..7618
                     /locus_tag="ACHKA1_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFO5682315.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGNVFNN
                     GRDVVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMALCKHAMNAGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLTVKGNGSNNQSALDYFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     YYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSQGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGTTASSTEYRTAEAAEPKSIIMKVWSGVNFADKIATSLSGTGTTSD
                     QLNPLIKMKNIGDYTMVTTETIWAGYTTVRVLSTAGSNSIFGRLVNAVSGLSDAAKAF
                     LEAAAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWAAT
                     HLGTNQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALFGAALVNV
                     QADSLTGLFSIAGFLMIYARACTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNMVS
                     SVKDIIAGGNTNIRRTPGVREEKLKNINGDDKDGIKQ"
     gene            7700..8347
                     /gene="excA"
                     /locus_tag="ACHKA1_004767"
     CDS             7700..8347
                     /gene="excA"
                     /locus_tag="ACHKA1_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFO5682316.1"
                     /translation="MNTTKSRYPSWWAYYQLARNTYFIFGIPCLLFYAFFGTLIVHDS
                     SLSQTRDFIFAYGCWALIILPLLWLYLKTRAKKKKVQQVVQQIKESSPFAPTNDYEQL
                     SFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSLP
                     MIPLEISGISARTLANTIHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            complement(8661..8813)
                     /locus_tag="ACHKA1_004768"
     CDS             complement(8661..8813)
                     /locus_tag="ACHKA1_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5682317.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWMVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            9146..9388
                     /locus_tag="ACHKA1_004769"
     CDS             9146..9388
                     /locus_tag="ACHKA1_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682318.1"
                     /translation="MSNEYQSTSINPDVFEQFLTLLNTHCKSSEAALAMLSTLCYELM
                     RRSGIHSLDSLMLNGEGILIQLQGADIREKTGQHIH"
BASE COUNT         2311 a         2665 c         2420 g         2045 t
ORIGIN      
        1 tttattttct ctgctgtttt ttcatgccac atcccctgct gtggcatcaa aaaatatcag
       61 accgtgatga ttcatcctta ccggagtgct ccatgccatg aatatcaacc gcattatttt
      121 tgccattgaa gactgtatca gtaccctgtc acggtattcc gtttccgcat tcttccccga
      181 ttactgtgat ctgcacaccg tcatcggtct ggaccaacag gaccgcgaac gccgcccctg
      241 gctgaaagcc ccctacattg ccaccaccaa acaggggtat tacctttccg tctttgaaat
      301 cagcggtgcc ctgaaagaaa tggatgaaaa cccggacctg acagcccccg gcacactgga
      361 atcactgatt acagcgctgg cagagcgaat gaacactgcc tataaaaaca tcggtcacaa
      421 aatcagtttt gttttcgaac gcgatccgga gcgtggaagg aatgaaatcg aagtcatgct
      481 cttaccccag cgaaaatcca tcgcacgcac ggggatcctg cttcaggacg ttctggatga
      541 aaaagtcacc acgctcaccc cctggcttgt ccgggaacgc tgctggctga ccgtctggag
      601 ctctgaagaa ctgctgagcc ggaccgatcg caaggaccac cagacccgta tcaggaaact
      661 cgctgaacac gctccgccag cccgatttgc gcaggatcca tggcgctgga cactttccgc
      721 actcaaaata cggcatgacg cctttctgga cacgctggag caggccctga cacatgattc
      781 tgatggtctg ctggttcgcc tgatggatat tcatgaagtg ggacgggaaa tccgccgtca
      841 actggagcgc aacagcacac ctgcagtatg gcagcctcat ctgcctgaag atgcccgtcc
      901 tgccggctgg cgacagggcg aggatacctc tgtattccac gcaccgtcgc tgaatctgca
      961 gttattcacc acacagcctg aaacacacgg cagcctgata aaggccggtg acctctggca
     1021 cggtatggtc gccattaccc tgccccccca gaatctgcgg acattcaacc agctggtcag
     1081 ggatgtgccc cgcgcaatcc cctggcgtat ccggatggat ctgatgcccg gcggcatgaa
     1141 ggcgcttggg gtcaagaaaa ccctgctgac ctacagcagc tttatttccc ctctccggcc
     1201 catgtatgac tcagtgatga tgctgaatga catcaacaaa catgatccgg tctgtatcat
     1261 gactattgtt gccaccacat ggggaaacag ccgggaagtc tgtacccgca accaggcgct
     1321 cctgaaatct gcgctggaag gctggggagt atgcggcacc accaccacct tcggtgatcc
     1381 gagacgtgcc tgggtgaaca cggttctggc ggcctcccac ggttccggtc cagtccccct
     1441 ttacccgccc ctttcacacg ccctttccct gtttcccctg aacagggccg gctctgtctg
     1501 gcggggacag ggaaatctga tgatgcacac cgaagacggc tctgcatggg aggtcgcgct
     1561 ggcatcgtca caacagaaca agcacacgga actgacgccc ggcgcacccg gtctgggcaa
     1621 atcggtactc atcaacgcac tgtcggagca gagaaaggac gaagccgtgg ggatcattct
     1681 gagcaatgat ccggaacaca cccgtaacct gtttgatgtg atgtacggtg ctaaaaaacc
     1741 cgccaccccg gaaaagaact tcatggtatc tgtactctgc gcactgtgcg tggataccgg
     1801 caccggtcag ccctgtaatc ccggtgacac ccggcaaatc ataagcaacc tggtggatct
     1861 ggccttcagg gagtatggcg aaaacaaccc cagactgtac cgtgcaggaa cagagcccct
     1921 tgtggatctc gccctggaag agagtggcat cgcagaacaa catgacgctg gatggtggaa
     1981 tgctgccacc tggtttgaaa tcagggacat gctgcatatg gctggcaata tccccgcagc
     2041 ccagcgggca cactaccagg ccatgcccct gctggctgaa atgtccgccc tgctgggaca
     2101 accatccatc cgggatgtgt tcggcacagt tcagcgcgat aactcagcag aattgttact
     2161 ggactacatc cgccgtgccc tcgatcaggg acacagtgac tacccgatga tgtccggctg
     2221 tacccggttt atgctcagcc ccgacacccg cgttgtggcc gttgatctga acaatgtggc
     2281 cggtgacaag acacctgccg ggcgtctgcg cactggcatc atgtacctgc tggcaggaca
     2341 aatcgccggc ggggattttg tcctgccgca gtatcaggag gaaatacgga aaaatctcga
     2401 cccacgctac catgaggtga ttttccgccg cattgaacag cttgatcagg aggtcaagac
     2461 caaagtctat gacgaactgc acaatgcaaa agggatcaat ttcatctggg aagccctgga
     2521 cacccaggag ctggagcagc gtaagttcgg gatacgcaca gtgctgagca cccagctatt
     2581 gcaggactat ccaccggcag ttctcaaatc cgccaacacc ctctggttgc tgcgttaccg
     2641 ccctgacgaa attccgtttc tgcgggataa ttttggtgtc cctgaagtca ccctgcggcg
     2701 cttcctgaaa atgccggaag gggctgcacc ggatggcagc ggtgtcccgg tactggctgt
     2761 gttccgtgta aaaaacggta cgctggcccg gatcctgaaa ttcactctcg gtccactgga
     2821 gctgtgggca ctgaattcct cacccaaaga cagcgccctg agacgtgcgc tgacgcagga
     2881 gctcgggtca ctgcgggcac gccagatcct ggcagaaaac tttccccgtg gttcagccac
     2941 ctcactgata gaacaccgcg cccgtacaca tgacagtgaa aacgtcatcc atgatctggc
     3001 tgcagaactc atccgtaagc agggatatca cctctgatgt acagcataca catcactccc
     3061 cgctgccggc gtcctgcacg gggacaggtc ttcctgaaaa cgctgcgcgt ggacggtgac
     3121 agtttcagct tcaccctgcc gtccgcctgc ttcacgctgc gtccctcccc caatccccgg
     3181 gtcattttcg ccagtgacag acgggctgac ggggaaatga gtggcgttcg cctggctctg
     3241 cccggagaga caggcacgct gattacaacg gccacctggc aggtcaacgt gccggagcgg
     3301 cctccttttg atatacagca tgtcattcac tggctcctgc cctcgcctct cacggcgctg
     3361 atgtccgaca acacctggct gtggcaggat agccccggca caccggcact gacggcggga
     3421 gactggccgg taatggaaac cgataccccg acgctgaaac gcgtgaagcg tcgtaccttc
     3481 acgctcatcg ataaccgcaa ccgtcaggga tggatcaccc gtcgcttcca gtgcttcccg
     3541 ctggctccgc tgccggaatc acacagtcac cgcgtaccag acatcctgta tcccctgaaa
     3601 acggagaaaa aagaatgcaa agacaaccag caaccacact gcttgcactc ctgataggct
     3661 gtgcggttat cccgtccacc cacgcctatt ctgtgaccgt agtggccagc cagcctgtca
     3721 ccacacaaat catgccggag ctggccgcca ttcagggcgt gatggcagag atggtccgta
     3781 tgcagacagc caccggcaca gccatcaacc agagttcaga gaaactggcc tctgtcattg
     3841 cgcaggatgg gcaggccacc cgtcagcaga tgatattcag taatgaaacc cgccggcttg
     3901 aggaagcccg gaagagcttc agcgtgccgg actccatctg cagtgaatcc gcctccggag
     3961 ttgcagctga aagtcgccgt gcagcagcgt ctgcggctgc acgtctgagt cagggaggag
     4021 gcgtcagcag taaaccaatc cgtgaacgtc tctcccgggc agcagactca ccaatccgcg
     4081 aggcatatga cagtgcaggc attcatgccg gttactgcac ggaagctgaa tatgcccgct
     4141 ttggcggaac cgatgtctgc cctgccgtgg gcgagctgcc gggcggagac agccaggtgc
     4201 gatcgctcta tcagggtgca ggcacagcag atacccctgc cgccctcacc tgggatcaga
     4261 aacagattga tgctgccacg gcttacatga aaaacaccgc cagaccgtca gcgggtcgtg
     4321 ccccggggaa aggtgaagtg ggaacacaga ccgggcgcac gtatgtgggc ctgcagaatg
     4381 aatacaacgg catcatcgat gccgcttctc atccgcaact gtcactgata gctgacagta
     4441 cccctaatga ggccacccgt ggagcgctga ctgaagccct gcagtcacca tccgctgcgg
     4501 catattttga ccggaccgca tcatcagagg cccgcacccg tggtcacatg agccagcggg
     4561 agtttgaggc ctttgaagct ggcagacgct atgcaaacac cgactggcag caggatctgc
     4621 agggcatgga aggcgataac cttctccgtg aactgctgcg cactaccgcc ctgctgaact
     4681 ggcagatgaa cgacctgaaa gagcagatac gccagggtaa tgttatcgcc gggcagcagc
     4741 tggcactggc ggcacgacag tattacggac agcgtctcgg tgaactgtca caggccatga
     4801 gccaggggag cgtaagatga aagacgacaa cagcaccgac aaaaaaaaac cggccccccg
     4861 tctccgccgc atcggtgaca tcgggataaa cctgttcgca ccactggcag aaacccggtg
     4921 gatgctgcgc agctgccgcc atatgctggg ccagcataaa acgcgcctga agcgcctcat
     4981 gcccgaccgg caaccggaga gtccggtctg tatgacctgg gaagaagccg tcagggccag
     5041 cggccttacg gtcaacgaac tggatcggcg tctgcgacgt cgcaggatgt tctggcggac
     5101 aggattctgg ctgatgatga taccggtact tctgctgtgc ggcatggccc ttgctgccac
     5161 agcgcttccg gccatcaccc tgatacgtat actggcctgc ctgttactga tgagtgccat
     5221 tgcagcactg tgtgcctcaa aaaccctgat tgtgtcatac cgcctctggc aactgaatga
     5281 acggcgggtc agcgaggacg aacagggcac cttccccgat tacctgcagg cccggcacgg
     5341 ctggaaacag gccacatggc tggccattac ccaccggcag tactgaccgt ccggtctgca
     5401 attccttttc ctcattccgg aggctgatgc cctgcatccc ttcggttccg gagttatcgt
     5461 gaaaatcata cccagagcac tgagtgcggg tctggcgctg gctgccgtac ctgcacaggc
     5521 attcgtcacc tatcaggaca ttgtgagcgc cgccaccaat ccggatgacc tgtcgcgcca
     5581 ggcactggtc accatttttg gcgacgtggt cacaaatccc ctgacagcca gcagcaacac
     5641 cattatcggt aacctgttca tggtgtttaa cgcaattatc gccattatgg ggatagtctg
     5701 gttcctgttt attggaatcc gccatgttgt ccgcaccggc caccagggca atgtctttaa
     5761 caacggacgg gacgtcgtcg gaatgctcag tattgtgagc ggttttatga tgattgtgcc
     5821 caccgcttcc ggctggagcc tggcacaact ggtcatgctc tggggcgcat ccatcatggg
     5881 cattggttcc gccaatatca tggttcagac tgctgcagaa aacatcgctg aaggctattc
     5941 aatgaccgta cagccagttc atgtctccac ccgcacggca gcacggggaa tactggagat
     6001 ggcgctgtgc aaacacgcca tgaatgcagg cctcgatgac tttaaccgta ctgcccgttc
     6061 atcaacgccc cccatgagcg aatcctcacg gacagagaac ggcaggtata ccgtcaccgt
     6121 ttcaaatggc agtgccacct gcgggagtgt gagtctcacc gtgaaaggga atggcagcaa
     6181 caaccagtcc gcactggact attttttcaa tcccttcatt gaaacggagt atcagggtgt
     6241 cctgacggca caacgctccg ccatggatac catgctccgt gacctggaca gcgcagcaca
     6301 ggaatttgtg tctgcgtacc ggacccgacg cgacacgggt aacggcaacc tgccggatat
     6361 tgaaagccgt attcagaagg ctgccaaccg gtatgaagaa acggtgcaga aggcgttgcc
     6421 aacagataac gcagaaactg agcgcaggga gcagctgaaa tactacctga ccacctacgg
     6481 ctgggttgcc ctgggggcct ggtaccagac ctttgccacc gccaaccagc gactggcaga
     6541 actggccgac cgtgccccca ctgtgagtgg agtttcatcg cagggcgaag ccggagatac
     6601 tgaattcctg aaggaagtaa aaggagctta ccaggcacag ttgcaaaact caccatatac
     6661 acctgcactg ggaacaaccg cttcatcaac tgaatacaga acagcagaag ctgctgaacc
     6721 taaaagcata atcatgaaag tatggtcggg tgtaaatttt gcagacaaaa ttgcaacatc
     6781 cttatctgga acaggaacca cctcagacca actaaatcct ctgataaaaa tgaaaaatat
     6841 cggtgattac actatggtca caacagaaac catctgggcg ggttatacca ccgtcagggt
     6901 actttccact gccggcagca actctatttt tggtcgactg gtcaatgccg ttagcggctt
     6961 atctgatgcc gcaaaggctt ttctggaagc agctgcacca ccgctctact tcctgctgtt
     7021 tttgctgttc tgtgccggtt tttcgttatc catttacctg ccgtttattc cgtttatctt
     7081 ctggatgacc ggcatcggta actggatagt cagtgtactg attggatgcg ccgccggtcc
     7141 cctgtgggct gccacccatc tggggacaaa ccaggaccgg ggaagccggg ctgcttacgg
     7201 ttacatctac ctgatagaca gcatgatcag accactgttg atggtgttcg ggttcttttt
     7261 tgcatcggtc gccatcgtgg ctggcggcac catactgaat gcgttgtttg gcgcggctct
     7321 ggttaatgtg caggctgact cccttacagg gttattcagt atcgcgggat ttttgatgat
     7381 ttatgcccgt gcctgtacca cgctggttgc ggccgttttt gcgcttcagg cttatctgcc
     7441 tgaccatgta attaattttc tgggcggacg tgacggcgtt aatatgctgg ggaatatggt
     7501 ttcatccgtc aaagatatta ttgccggtgg caacaccaat atacgcagaa cacctggcgt
     7561 tcgtgaagaa aaactgaaaa acattaatgg agatgataaa gacggaataa aacaataaag
     7621 tacagacatc aaaaaaaaca gtattgccac attcacctga agagattaaa atacattcag
     7681 gtgaatatgg aggtttatta tgaacacaac aaaaagcaga tatccttcct ggtgggcata
     7741 ttatcaacta gcccgcaata cttactttat atttggaatt ccatgcttat tattctatgc
     7801 ttttttcgga acactaattg ttcatgactc aagcttatct caaacaagag attttatttt
     7861 tgcatatgga tgctgggcat taataattct cccattactc tggctatatc tgaaaaccag
     7921 agcgaagaaa aagaaagtcc agcaggttgt tcagcagata aaagaaagca gtccctttgc
     7981 accgaccaac gattacgagc aactgtcatt ctcgaagagt tgttatttcg ggatcgacat
     8041 taaaaacggc accatgctgt atgtgcgcat ttacccaaac aatgtcatgg atgtgattgg
     8101 cctggatatt cacaacttca cccgaacagt tgctgaagac ggcaagctgg aaatccacac
     8161 cacctatgtg agtctgccga tgatccctct tgaaatctcc ggcatctcag caaggacgct
     8221 ggccaacacc atacacacca tggctgcacg tggatatgag tacaaagagc gtttcccgca
     8281 gatgatccgg tatcgggtta aagagtggga aaaagttgcc ggtgttcctg ttgcagaagt
     8341 attttgaggt agctatcaac tgttagacga taatccgtca gccggttttc tctgaaccgg
     8401 ctttttcttt tcaggcagaa tgcctgtctg ccgtccctgt atcagactgt aattagcaca
     8461 atacggcaat ttgccgttca gtatattcag gaagtacaaa tcatgccatc tgcaacacaa
     8521 actgcccgac tggaagcaag gatcagcaca gaactgcatg ctatgctgaa acgcgctgca
     8581 gaaatccagg taaaaaaggg cgcattgagg tttgctttcg caacaatccg gcggcagatt
     8641 tcctgccgcc gtgttgccca ttaacgttta acttcgtagg ctaacgttgc cacaagcact
     8701 gtgtttccct gctcgacacg gaatccgcaa agcgaatccc tcaccatcca gacaaaacac
     8761 agtatcgtca cacagacgac aattaacatt gttaaaaacg ttcgttgtgg catcgcagcc
     8821 cctccattgc ctttcggccg tcatcgggct actataatgt tgtctaggca ttacagtggg
     8881 ccctgggttg attgataaaa aaatcgcctg gggctttctg ctgcatgccg tccacaaaac
     8941 gctttcagca tacagcctca agcgcccgcc gctattctaa tcgattttca gtctgccaca
     9001 atcgttttcc acctaatgac atgttatgca caacagtatt tttacgggaa actacacggc
     9061 aaggattccc ggaacacgtt cattatttga tggttacaga catcatgtta aatccggaag
     9121 taataatgac ctgaaaggaa atactatgag taacgaatat caaagcacca gtataaaccc
     9181 tgatgttttt gaacaatttc ttaccctcct gaatacacac tgtaaatcat cagaagctgc
     9241 acttgccatg ctgagtacac tctgctacga actgatgagg aggagtggga tccattcact
     9301 ggacagcctg atgctcaacg gagaaggtat attgatacaa ttacaggggg ctgatattcg
     9361 cgaaaaaaca ggtcagcata ttcactgatc cagggatgtg ctgaaaacag tgtcagcctg
     9421 aaataaacgt tcaatgcctc a
//
LOCUS       DAZFKF010000155         9438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFKF010000155 DAZFKF010000000
VERSION     DAZFKF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.157"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1312
                     /gene="traB"
                     /locus_tag="ACHKA1_004770"
     CDS             <1..1312
                     /gene="traB"
                     /locus_tag="ACHKA1_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFO5682319.1"
                     /translation="NTDAVAGQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQ
                     YEEIRREMDVLNKQRGDDQRRIEKLGQENAALAEQVKALGANPVTVTGEPVPQMPASP
                     PGPEGEPQPGNAPVAFPPQGAGAVPPPTAFYPGNGATPPPQVTYQSVPVPNRIQRKTF
                     SYDAGTKQTPSLPYIPSGSFAKAILIEGADANASVTGNESTVPMQLRITGLVEMPNSK
                     TYDATGCFVGLEAWGDVSSERAIVRTRNISCLKEGKTIDMPVKGHVSFRGKNGIKGEV
                     VMRNGKILGWAWGAGFVDGIGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAA
                     QTLSDYYIKRAEQYHPVIPIGAGNEVTVVFQDGFQLKTVEEMALEQAQGQVEESNPGS
                     PVPNPPSAESHLNGLNTDQMLKQLGDLNPQQFMAGGQEGGNDGK"
     gene            1302..1886
                     /gene="traP"
                     /locus_tag="ACHKA1_004771"
     CDS             1302..1886
                     /gene="traP"
                     /locus_tag="ACHKA1_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFO5682320.1"
                     /translation="MANNTSSSQACHTARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVQEIDFIRQTAPAGQFPVRECLPSLPDLPGAEEKICRYRAADA
                     ADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYLVRPGKMKCIRVVTADEHLKEV
                     YTEDASLPGKIRKCHAYFPDNRTNRNNGDKNEHA"
     gene            1873..2067
                     /locus_tag="ACHKA1_004772"
     CDS             1873..2067
                     /locus_tag="ACHKA1_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFO5682321.1"
                     /translation="MNMRNIHVITALSVPDKRISDDFMHAVLSNCTTGVLPAPEKFGS
                     ESLPDNFTMTAVGVMKNSEI"
     gene            2079..2330
                     /gene="trbG"
                     /locus_tag="ACHKA1_004773"
     CDS             2079..2330
                     /gene="trbG"
                     /locus_tag="ACHKA1_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HFO5682322.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRNTVFENPDIVKTCTVRDLKNDFVNCDEKGEGQ"
     gene            2327..2842
                     /gene="traV"
                     /locus_tag="ACHKA1_004774"
     CDS             2327..2842
                     /gene="traV"
                     /locus_tag="ACHKA1_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO5682323.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNSDFECDATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLQRLAEGNFRTMPVQTVTATSPSGSRPAVTALPEQKLLAPR
                     PLFTAAREVKTVVPVSSVAPVIPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            2977..3198
                     /gene="traR"
                     /locus_tag="ACHKA1_004775"
     CDS             2977..3198
                     /gene="traR"
                     /locus_tag="ACHKA1_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFO5682324.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            3191..3664
                     /locus_tag="ACHKA1_004776"
     CDS             3191..3664
                     /locus_tag="ACHKA1_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682325.1"
                     /translation="MHKSENEKIDSILDEHQQIYRRHNRIDNILAFFACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFTEGNKSE"
     gene            3744..3962
                     /locus_tag="ACHKA1_004777"
     CDS             3744..3962
                     /locus_tag="ACHKA1_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682326.1"
                     /translation="MHTFIFLVLMKNVGGGGSWNVTPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNNVMETLNGGGL"
     gene            3990..4337
                     /locus_tag="ACHKA1_004778"
     CDS             3990..4337
                     /locus_tag="ACHKA1_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682327.1"
                     /translation="MKSIYLKYFLSFVFVGVMAMIVCIPFYIIYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTFGKANKIASECRKRNEMEEARRVRENELNKIREKQ
                     LQALSDADSAKER"
     gene            4468..7098
                     /gene="traC"
                     /locus_tag="ACHKA1_004779"
     CDS             4468..7098
                     /gene="traC"
                     /locus_tag="ACHKA1_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO5682328.1"
                     /translation="MSNNPLEVVTQAVNSLLTALKLPDESAQANQILGEMNFPQFSRL
                     LPYRDYNQESGLFMNDSTMGFMLEAIPINGANETIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGERIEYGLREFSWSGEQAERFNAITRAYYMKAAETLFPLPEGLNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGAYITTQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLTRQNRARIDDVVDFLKNARDNDQYAESPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            7095..7481
                     /gene="trbI"
                     /locus_tag="ACHKA1_004780"
     CDS             7095..7481
                     /gene="trbI"
                     /locus_tag="ACHKA1_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO5682329.1"
                     /translation="MTTTQKTTDVTARRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNT
                     PVTVAFNMKQTVDAFFDSASQKKLSEAQSKALADRFNAALEASLQTWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            7478..8110
                     /gene="traW"
                     /locus_tag="ACHKA1_004781"
     CDS             7478..8110
                     /gene="traW"
                     /locus_tag="ACHKA1_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFO5682330.1"
                     /translation="MRCRGLIALLVWGQSVAAADLGTWGDLWPVKEADMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RIFARQGEVMNPLQYVPFNQTLYFINGDDPEQIEWMKRQTPPTLESKIILVQGSIPEM
                     QKALDSRVYFDQNGVICQRLGIDQVPARVSAVSGDRFLKVEFIPVGEGGK"
     gene            8107..9099
                     /gene="traU"
                     /locus_tag="ACHKA1_004782"
     CDS             8107..9099
                     /gene="traU"
                     /locus_tag="ACHKA1_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO5682331.1"
                     /translation="MKRCMWLCVVLLLTGHVPAASADAACEGRFVNPITDICWNCIFP
                     LSLGSTKVSQGKVPDTINPSMPIQFCPAPPPVFKRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGITIPELGKTGQGTAKKDDKQVNGAFYHVHWYKYPLTYWLSIITSAGCLMGGDMD
                     IGYISEIDPTWTDSSLTTILNPEAVIFANPIAQGACAVDAIASAFNKPLDILFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMNSIGVDVAVCYEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            9113..>9438
                     /locus_tag="ACHKA1_004783"
     CDS             9113..>9438
                     /locus_tag="ACHKA1_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682332.1"
                     /translation="MRNSKVQLVSILRQVSLSLNTEPLRQFISLREIAEETDHVAARL
                     SGGKRVTPAQIYELCAQLWMARMKAVEVYGRHSDVVMSLERQTDLLEAAGNVLKQRWF
                     YRPWGSS"
BASE COUNT         2371 a         2231 c         2664 g         2172 t
ORIGIN      
        1 gaacactgat gccgttgcag ggcaggagcc tgtgccggat atgaccggtg ttgtggatac
       61 gacctttgat gacaaggtgc gtcagcatgc caccacagag atgcaggtga cggcggcaca
      121 gatgcagaag cagtatgagg aaatccgtcg tgagatggat gttctgaaca aacagcgtgg
      181 tgatgaccag cgtcgcattg aaaagctggg gcaggagaat gctgcgctgg cagagcaggt
      241 gaaagccctg ggcgcgaatc ccgttacagt gaccggagag cctgtgccac agatgccggc
      301 atcccctccc ggtccggagg gggagcctca gcccggtaat gcgcccgttg cttttccacc
      361 gcagggggca ggtgcagtgc caccgcccac cgcgttctat ccgggcaacg gagccacgcc
      421 gccgccacag gtgacgtacc agtctgtgcc ggtgccgaac cggatacagc gtaaaacgtt
      481 cagctatgat gctggcacaa agcaaactcc gtccctgccg tacattccgt caggcagttt
      541 tgcgaaagcc attctgattg aaggggcgga tgccaatgct tctgtcaccg gtaatgaatc
      601 cacggtgccg atgcagctgc gtatcaccgg tctggtggaa atgccgaaca gcaaaaccta
      661 tgacgcaacg ggatgctttg ttgggctgga agcctggggg gatgtgtcca gtgagcgtgc
      721 catcgtgcgt acccgcaata tcagctgcct gaaggagggt aaaaccatcg atatgccggt
      781 taaagggcat gtcagcttcc ggggtaaaaa cggtatcaag ggtgaagtgg tgatgcgtaa
      841 cggcaaaatc ctcggctggg catggggcgc gggatttgtt gacggtatcg gtcagggaat
      901 ggagcgcgct tcccagccgg ctgtcgggct gggtgccact gccgcttacg gggccggcga
      961 tgtcctgaaa atgggtatcg gtggcggtgc atcgaaagcg gcacagacac tcagtgacta
     1021 ctacatcaaa cgcgctgagc agtatcaccc ggtgataccc atcggggcgg gtaatgaagt
     1081 gacggtggtg ttccaggacg gtttccagct gaaaaccgtg gaagagatgg cgctggagca
     1141 ggctcagggg caggtggaag agagtaaccc gggaagtccc gtgccgaatc cgccatccgc
     1201 agagagtcac ctgaatggtc tgaataccga ccagatgctg aagcagctgg gcgacctgaa
     1261 tccgcagcag tttatggccg gtggtcagga agggggcaac gatggcaaat aatacgtcgt
     1321 ccagtcaggc gtgccatacc gcccggtatg ttgtcgcccg ggttctgcgt ggactgttct
     1381 ggtgtctgaa atacaccgtc attctgccac tggccacaat ggccctgatg gcgttatttg
     1441 ttttatggaa ggacaacacc acaccaggga aattactggt tcaggaaatt gacttcataa
     1501 gacaaacagc ccctgccggg cagtttcctg taagagaatg tttgccttct ctgccagatc
     1561 ttccaggagc agaagagaaa atctgccgtt accgtgctgc tgatgctgca gattatgtca
     1621 gggagactga ccggtcactg atgcaactgg tcaccgcatt atgggcaaca ctggcactga
     1681 tgtatgtctc gctggcagca atcaccggga aatatctggt gcgacccgga aaaatgaaat
     1741 gtatccgggt ggtaaccgca gatgaacatc tgaaggaagt ttatacggaa gacgcttccc
     1801 tgccgggaaa aatcaggaaa tgccatgctt atttccctga taacagaaca aacagaaaca
     1861 acggagataa aaatgaacat gcgtaatatt catgttatta cggccctctc tgtaccggat
     1921 aaacgcatat cagatgattt tatgcatgct gttctcagta actgcaccac aggagtactt
     1981 cctgcaccgg agaaattcgg ttctgagtca ttgccggaca attttactat gactgctgtc
     2041 ggtgtgatga agaattcaga aatataagga acccttcgat gaataagctg gtttctgatg
     2101 gcagcgtcaa aaaaataaat tatccggtgc tgtatgagtc cggaataaca cctccgttat
     2161 gtgaagtcag tgcaccagaa cctgatgcgg gtgggaaacg cattgtggca tatgtttata
     2221 aatcatcacg gaatacggtt tttgaaaatc ctgacattgt aaaaacctgt acggtcaggg
     2281 atttgaagaa tgattttgtg aactgcgatg agaaaggtga gggacaatga aacagatttc
     2341 tttatttatt ccgctgctgg ggacgttatt actttccggc tgtgccggaa caaactccga
     2401 ctttgagtgt gatgccacca catccgatac ctgtatgacg atggagcagg ccaatgagaa
     2461 ggccaaaaaa ctggagcagt cctcagaagc aaagccggtt gcggcatcac tgcagcgcct
     2521 ggctgaaggg aacttccgga caatgccggt gcagaccgtc accgcgacct ctccgtccgg
     2581 cagcagacct gcggtgacgg cacttccgga gcagaaactg ctggcaccac gtccgctgtt
     2641 taccgctgcc cgggaagtga aaacggttgt tccggtcagt tcagttgcac cggtaatacc
     2701 tccccgtccg ctaagaacgg gtgagcagac ggcagcatta tggatagcgc cttatattga
     2761 taatcaggat gtttatcatc agccatccag tgtgtttttt gttattaaac cgtctgcgtg
     2821 gggaaaacca cgtattaatt aacaggccct gaagttgtat atcagccggc aatgccggcg
     2881 ggctttctgt attcaaaatc tgcctgtaaa acgagtgccc tgtgcgtgaa aagggatggg
     2941 cggtagcgtg tcagaacatg tcgggaggaa tattccgtga gtgatgaagc cgatgaagca
     3001 tattcagtaa cagaacaact gaccatgaca ggaataaacc ggatacgcca gaaaataaat
     3061 gttcatggta ttcctgttta tctctgtgaa gcatgcggaa atcctattcc ggaagcccgg
     3121 cggaaaatat ttcccggtgt gacgttgtgc gttgaatgtc aggcgtatca ggaaaggcag
     3181 aggaaacact atgcataaat cagaaaatga aaaaatagat tctattctgg atgaacatca
     3241 acagatatat cgcagacata accgaataga taacatcctg gcgttttttg cgtgtattgc
     3301 atggattccc tgtattattg ctgcgttcgg cgttgatggt atatatctga aaatcctgtt
     3361 tgcggtcctt gcctgctgtg gtgcggtatg cttttttatt ttcttcttca ctctcctgcc
     3421 ggaatcactc atggtcctca gccgtcagag cctgcttcag cttatgcgtc tgactgaaga
     3481 cgtaccggat gcgagacagg aactgcttaa ccgtctgctg tccggtaaaa aactgactgg
     3541 tcgggatgaa aaggatatcc ggcgtctctg gcaggagaaa gtggatgcaa tgcaggagtc
     3601 tgccacccgt cagcgtgaac aggataccat ccggaagttt actgaaggga ataaatccga
     3661 atgaaaatct gctgtgtttt ataacctcat aagttaactg aaggagtatt cagtgattaa
     3721 tatttcactg gaggtaataa agaatgcata cctttatttt tcttgtgctg atgaaaaatg
     3781 ttggcggtgg cggaagctgg aatgttaccc ccatgccaaa tacggacgta tgcgtgcagg
     3841 ttcttcggac aatcgaagca catggaggca ggggatttgg aaatacaggg gagtgtattg
     3901 aagttaaaac taatgaacct ttgaataacg tgatggaaac actgaatggt ggtgggctat
     3961 gagtgcttac tgcttcgaag gaaaatgaaa tgaagtcgat atatttaaaa tattttctgt
     4021 cttttgtatt tgttggtgtt atggctatga ttgtatgtat tcctttttac atcatttacc
     4081 ttgcgcaaca acctgcaaca ccagaacaac tgacagagat tttacaggaa actccctgtg
     4141 cagcagaagc atttcaggag acacttaatt atcagtctga acctttaaca tttggtaagg
     4201 caaataaaat cgcatcagaa tgcagaaaaa gaaatgaaat ggaagaggcg cgacgggtaa
     4261 gagaaaacga gctcaataag atacgcgaaa aacaacttca ggcattaagt gatgctgatt
     4321 cagcaaagga acgttaattg taatcctggt ttcctgttaa aagcctccat ctcttatttg
     4381 tgctgtggtt tcagaaatgg cactgcgtga tatatccacg ccacagactg atattttcct
     4441 tttttaaacg tactaaggaa tactcatatg agtaataacc cacttgaggt cgtcactcag
     4501 gcggtcaatt cactgctgac cgcacttaaa ttaccggatg aatctgctca ggctaatcag
     4561 atcctcggtg agatgaactt cccgcagttc agccgtctgc tgccataccg tgattacaac
     4621 caggaatccg gtctgttcat gaatgactcc acaatgggct ttatgctgga agccattccc
     4681 atcaatggtg cgaatgagac cattgtggag gctctcgacc atatgctgcg caccaaactg
     4741 ccgcgcggta ttccgctgtg tatccatctg atgtccagcc agctggttgg cgagcggatt
     4801 gagtacgggc tgcgtgagtt ctcctggtca ggtgaacagg cagaacggtt taacgccatt
     4861 acccgggcct attacatgaa agccgcagaa acactgtttc cgctgccgga agggttgaac
     4921 ctgcctctga ccctgcgcca ttaccgggtg tttatctcgt actgttctcc ctcgaagaaa
     4981 aaaagccggg ccgacattct ggaaatggaa aacctggtga aaatcatccg ggcgtctctg
     5041 cagggagcat atatcaccac gcagacggtg gatgcacagg ctttcatcga tattgtcggg
     5101 gagatgatta accataaccc ggactccctg tacccgaaaa gacgtcagct ggacccgtat
     5161 tctgatttga attatcagtg cgtggaggac agttttgacc tgaaggtccg ggcagattac
     5221 ctgacgctgg gactgcgtga gaacggcagg aacagcactg cccgcatcct gaatttccat
     5281 ctggcccgta acccggaaat cgccttcctg tggaacatgg ccgacaacta cagcaacctg
     5341 ctgaacccgg aactgtccat ctcctgcccg ttcattctga cgctgaccct ggtggtggaa
     5401 gaccaggtga aaacccacag cgaagccaac ctgaagtaca tggacctgga gaaaaagtcg
     5461 aagacctcct atgccaaatg gtttccgtcc gtggagaaag aagcgaagga gtggggggaa
     5521 ctgcgtcagc ggctgggctc cggtcagtct tctgtcgtgt cctacttcct caacatcaca
     5581 gcattctgca aggacaataa tgaaacggca ctggaagtgg agcaggatat cctgaacagc
     5641 ttccgtaaaa acggttttga actgatttca ccgcgcttta accacatgcg caatttcctg
     5701 acctgcctgc cctttatggc cgggaaaggg ttgtttaaac aactgaaaga ggccggagtg
     5761 gttcagcgcg cagagagctt taatgtggcc aacctgatgc cgttagtggc ggataacccc
     5821 ctgacgccgg caggtctgct ggcacccacc taccgtaacc agctggcgtt tatcgatatt
     5881 ttcttccggg ggatgaataa caccaactac aacatggcgg tctgtggcac ttccggggcc
     5941 ggtaaaaccg ggctgataca gccacttatc cgcagtgtgc tggactccgg gggttttgct
     6001 gtggtgttcg acatggggga tggctacaag tccctgtgtg aaaacatggg gggcgtatat
     6061 ctggacggtg aaaccctgcg ctttaacccg tttgcgaaca tcaccgatat tgaccagtca
     6121 gcggagcgtg tccgtgacca gctgtcggtg atggccagcc ccaacggtaa cctggatgaa
     6181 gtgcatgaag gtctgctgct gcaggcggtc agggcctcct ggcttaccag acagaaccgg
     6241 gcgcgtattg atgacgtggt ggatttcctg aaaaacgccc gggataacga ccagtatgcc
     6301 gagtccccga ccatccgcag ccgtctggat gaaatgatag tgctgctgga ccagtacaca
     6361 gccaacggta cttacggcca gtattttaac tctgatgaac cgtccctgcg ggatgacgca
     6421 aaaatggtgg tgctggagct gggtggactg gaagaccgtc cgtccctgct ggttgcggtg
     6481 atgttcagcc ttatcattta catcgagaac cggatgtacc gcacgccgcg taacctcaag
     6541 aaactgaacg tcattgatga ggggtggcgt ctgctggact tcaaaaatca caaggtcggt
     6601 gagtttattg agaaaggtta ccgtaccgcc cgccgtcata ctggtgccta tatcaccatc
     6661 acacagaaca tcgtcgactt tgactctgac aaggcctcca gtgcggcccg tgcggcatgg
     6721 ggtaactcct cctacaaaat tatcctcaaa cagagtgcga aggagttcgc gaaatataac
     6781 cagctgtatc cggaccagtt cctgccactg cagcgcgaca tgattggtaa gtttggtgcg
     6841 gccaaagacc agtggttcag ttccttcctg ctgcaggtgg aaaaccactc ctcctggcac
     6901 cgtctgtttg tggacccgct aagccgtgcc atgtacagct ctgacggccc ggattttgag
     6961 tttgtgcagc agaaacgtaa agaggggctg agtattcatg aggcggtgtg gcagctggcg
     7021 tggaagaagt cagggccgga gatggcttcg ctggaagcat ggctggaaga acatgagaaa
     7081 tacaggagtg ttgcatgacc acaacgcaaa aaacgactga tgtgacagcc cgtcgacgca
     7141 gccactggtg gtggacggtg ccgggatgcc tggcaatggt gttactgaac gcagccgtca
     7201 gttacggcat tgtcagactg aacacgccgg taaccgtcgc cttcaacatg aaacagacag
     7261 tggatgcgtt ttttgacagc gccagccaga aaaaactgtc agaagcgcag tcgaaggcgc
     7321 tggctgaccg ttttaacgcg gcactggagg ccagtctgca gacgtggcag cagaagcacc
     7381 acgctgtcat tctggtgtcg cctgccgtgg tacagggggc accggatatc acccgtgaaa
     7441 tccagcagga tattgcccgg cgaatgaggg cagaaccatg agatgccggg ggctgattgc
     7501 cctgctggta tgggggcaga gtgtggctgc agctgatctt ggcacctggg gggatttgtg
     7561 gccggtaaag gaggcggaca tgctgacggt gattatgcag cgtctgacag ctctggagca
     7621 gtccggagag atgggccgga aaatggatgc gtttaaggag cgggtgatcc gcaacagcct
     7681 gcggccccct gccgtgcccg gtatcggtcg gacggagaaa tacagcagcc gcctgtttga
     7741 cccgtctgtc agactggctg cggatatccg ggataacgaa ggtcgtatct tcgcccgcca
     7801 gggggaggtg atgaacccgc tgcagtatgt gccttttaac cagacactgt atttcatcaa
     7861 cggtgatgat cctgagcaga ttgaatggat gaagcgccag acgcctccca cactggagag
     7921 caaaattatc ctggtgcagg gcagtatccc ggagatgcag aaggctctgg acagtcgtgt
     7981 ttactttgat cagaacggcg tgatctgcca gcgtctcggc attgatcagg tgccggcacg
     8041 ggtgagtgct gtcagtggcg atcgtttcct gaaggtggag tttattccgg tcggggaggg
     8101 agggaaatga aacggtgtat gtggttgtgt gtggttttgc tgctgactgg ccatgttccc
     8161 gcagcctcag cggacgctgc ctgtgaaggg cggtttgtta acccgatcac ggatatctgc
     8221 tggaactgca tttttccact gtcgctgggc agcacaaaag tgagtcaggg gaaggtgccg
     8281 gatacaataa acccgtcaat gccgatccag ttctgtcctg cgccaccgcc tgtatttaaa
     8341 cgtatcgggc tggctattgg ttactgggag ccgatggcac tgacggatgt gacccgcagt
     8401 ccgggatgta tggtaaatct tggaataaca atcccggagc tggggaaaac cgggcaggga
     8461 acggccaaaa aagacgacaa gcaggtgaac ggggcatttt accatgttca ctggtacaaa
     8521 tacccgctga catactggct gagtattatc acctcagcag gatgtctgat gggcggcgat
     8581 atggatatcg ggtacatctc agaaattgac cctacctgga cagacagcag tctgaccacc
     8641 attctcaacc cggaagcggt catctttgca aatccgatag cacagggagc ctgtgcggtg
     8701 gatgccattg ccagtgcctt taacaagccg ctggatatcc tgttctggtg tgccggttcg
     8761 cagggcagta tgtatccgtt taatggctgg gtgagcaatg aatccagtcc gttgcagtca
     8821 tccctgctgg tcagtgaacg gatggcgttt aagctccacc gtcagggaat gataatgaac
     8881 agcatcggcg tggatgtggc tgtctgttat gaatatccgt cgcccatact tcccaaagag
     8941 cgctggcgtt atcagatggt gaatatgtac ccggacagcg gacagtgcca tccgttcgga
     9001 cgcagtgtga tgcgctggga gacgggaaaa aatccgccca acacaaagaa aaacttcggt
     9061 tacctgatgt ggcgtaaacg taactgtgtg ttcctgtaat cgggaaaatg tgatgcgcaa
     9121 cagtaaagtg caacttgtat caatcctcag acaggtcagt ctttcactga acacggagcc
     9181 attacgacag tttatctctc tgagggagat tgcagaagag acggatcacg tcgctgctcg
     9241 tttgtccgga ggaaaacggg taacacctgc acaaatttat gaattgtgtg cacagttatg
     9301 gatggccagg atgaaggcag ttgaagttta tgggcgacat tctgatgtgg tgatgtctct
     9361 ggaaagacag actgatttac ttgaggctgc aggcaacgta ttaaaacaac gctggtttta
     9421 ccgtccgtgg ggatcatc
//
LOCUS       DAZFKF010000156         9366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFKF010000156 DAZFKF010000000
VERSION     DAZFKF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.158"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            45..647
                     /gene="lpfA2"
                     /locus_tag="ACHKA1_004784"
     CDS             45..647
                     /gene="lpfA2"
                     /locus_tag="ACHKA1_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA2"
                     /protein_id="HFO5682333.1"
                     /translation="MKPNMIVGALALTSVFMAGHLQAADGTVHFRGEIIDSTCEVTPE
                     TKDQVVDLGKVNRTAFSGVDDVAAPTAFSIDLTQCPETFKSAAIRFDGNEDAHGNGNL
                     AIGTPLDNSNDAAAGISPSDNSGDYTGAGAVSAAKGVAIRLYNRADNTQVKLYENSAS
                     TPISNGNASMKFMARYIATETTIDPGTANADSQFTVEYIK"
     gene            756..1436
                     /locus_tag="ACHKA1_004785"
     CDS             756..1436
                     /locus_tag="ACHKA1_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO5682334.1"
                     /translation="MYLLRLFFAVTLITQMSMAMAGIVVGGTRVIFYGDKPDTTISIF
                     NKETNLPYLLQTWVDPFNKEDKSKPPFSVIPPVSRIEPQQEKILRILKTAGELPKDRE
                     SVFWLNVKNIPPSIDKKNTNVLEIAIKTRIKLFWRPAAVKIIPEQAIKNVKWSRQGNN
                     LIVENPNPIHINVMSVTVDDKDVSLNMIRPFETLTLPIPAGVAGKSLVWRFINDYGAI
                     SQPLKKNL"
     gene            1458..3992
                     /locus_tag="ACHKA1_004786"
     CDS             1458..3992
                     /locus_tag="ACHKA1_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5682335.1"
                     /translation="MASSHLFITLASGICLLCSISAFARDSLFNPRLLELDHPADNID
                     IHQFNRSNTLPAGTYKVDVMINGMLFERQEVKFVQDNPDAELHPCYVAIKNVLATYGI
                     KVDAIKSLANVDDKTCVNPVPLIDGATWLLDASKLALNITIPQIYLNNAVNGYISPSR
                     WDQGINAMMMNYDFSASHTIRSNYDDDDDSYYLNLRNGINLGAWRFRNYSTLNSYDGN
                     VDYHSVSNYIQRDIMALRSQIMIGDTWTASDVFDSTQVRGVRLYTDDDMLPSSQNGFA
                     PVVHGIAKTNATVIIKQNGYVIYQSAVPQGAFALTDLNTTSSGGDLDVTIKEEDGSEQ
                     HFIQPFTSLAILKREGQTDVDLSIGEVRDESGFTPEVLQLQAMHGFPLGITLYGGTQL
                     ANDYASAALGIGKDMGALGAISFDVTHARSQFDYGDNESGQSYRFLYSKRFEDTNTTF
                     RLVGYRYSTEGFYTLNEWVSRQDNDSDFWVTGNRRSRFEGTWTQSFTPGWGNIYLTFS
                     RQEYWQTDEVERLLQFGYNNNWRNISWNVSWNYTDSIKRSSSNYHDDNNDNFGKEQIF
                     MFSMSIPLSGWMEDSYVNYSLTQNNHHESTMQVGLNGTMLEGRNLSYNVQESWMHSPD
                     DSYSGNAGMTYDGTYGSVNGSYSWSRDSQHFDYGARGGVLVHSDGVTFSQELGETVAL
                     VKAPGAEGLSIENATGISTDWRGYTVKTQLSPYDENRVALNSDYFSKANIELENTVIN
                     LVPTRGAVVKAEFVTHVGYRVLFNVRQVNGKPIMFGAMATASLETGTVTGIVGDNGEL
                     YLSGMPEKGEFLLSWGQAADEKCKAAYHITHKPDDTSLIQMDAICR"
     gene            4004..5095
                     /locus_tag="ACHKA1_004787"
     CDS             4004..5095
                     /locus_tag="ACHKA1_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5682336.1"
                     /translation="MKKILSGLILLLCCPYGFAANGDGVCFNSDGATHMSNLSFGPLT
                     VAAANNHSGYNIFEALSNTTGTYPVRCHCDDTHGGPGQQTAFFPIFYTGDAAPGLVLE
                     RTLNGLNYYALNDYLSVGVTIFIINNQYAAIPFEHLSNQSTSPQHTCGAGNNGSTVNL
                     DSGRSAKLSFYVRHSITGTVTIPTTEVAWLYAGMSDHFPKTTPVSKVTIRGQLTAPQN
                     CELTPNQSIDVDFQKINSGEFSSTAGSIIAERKIKTEVTVSCTGMEDVRSTEVVSASM
                     IAANRSADATMIVTSNPDVGIKIFDKNDRPVNVDGGNLPADMGAISRLGKTDGSVTFY
                     SAPASLTGTKPAPDNGFTATATLVIEFTN"
     gene            5123..5881
                     /locus_tag="ACHKA1_004788"
                     /pseudo
     CDS             5123..5881
                     /locus_tag="ACHKA1_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(5940..6521)
                     /locus_tag="ACHKA1_004789"
     CDS             complement(5940..6521)
                     /locus_tag="ACHKA1_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5682337.1"
                     /translation="MALLGYARVSTSHQKLTHQISELKNAGVREDRIFTDMMTGATDE
                     REGLQRLLARAEKDDVIICTKMDRLGRNTADMIRVVDSCYKKGIAIRFLENGLSTEGT
                     MGKMVIQILAAVAEAERERILERTNDGRIAALAAGVKFGRKKHPRTPTALELISQGES
                     LGSVTEKTGISRSTYFRLKRTIKNDAKIATFYK"
     gene            6678..>9366
                     /locus_tag="ACHKA1_004790"
     CDS             6678..>9366
                     /locus_tag="ACHKA1_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFO5682338.1"
                     /translation="MQKYKKRISILTKSEINELYQVPSFNPVERDEFFSLDISLKREI
                     DKIINIESRVYLILITGYFRYKPVIPEFTLKDVKRDVKYIIQTYYKNESTNLNLNIPK
                     STKYRLVKRMLSTLGFEQLTPSIKNELSERLNDVATICMEPKYIFDELLAFLGQRRIA
                     LPGYSTVQNLVSDALNFERKRIINILSKRIYPATAQKITSIIHDDGILNSIRGYNYST
                     RDFSASEIENELRTHNVISSIYYEIKDIINSLGISQGNMEYYATIVKHSTTFRLKRHP
                     KWQGILYLCCYLYFRYREINDKIITAFRYLVKKHKDASVLFAKARATEEIDLINKKLK
                     FASKIFNFFVDSNIEDSIPFGYVREKVFSLISKDDMHLVSEFFDKNKIDVIEYQWQYI
                     DSHHLKIANSIRKLFLAIDIVYDNEKSVIGQQITAAKDDLRGKGKLDAKDLRVIHPSE
                     KNYIVKEGEINTQRFEFHMYSRVSNHLENGVFYAAESEQNKRLEDDLIERSYWHKNKS
                     ILIEKTGLERLITPITQTLNELENTFSDKLARITIGINSDANEFVKKQPHSNRLQWSL
                     ASKKWKTSIDNPIYNQIKNIGIVDVMKYVNNETNFLSVFNAISSRKNNLEADYNDLLA
                     CIFGNGANYGVHRMASASDRSIGILRDVNDKFIRPETTGMANTVISDAIANLSVYKYW
                     TINEDSPFGSVDGQKHTCRINTFKARYSAKYFRKGKGVSAMTLVSNHVPLSTVVISPN
                     EYEGHFAFDLLYNNTSEIQPKSLATDNHGINNVNFAILDIFGYQFSPRYAKFKKIFNE
                     LFDVILESDKLIIKLKKQFNFKLIEHEWENIQHIICSLSRKTTIQSTVVKKLSNTKRS
                     NKTLAALREYDRVIKCIYVLDYADNKTLRQFVQQALNRGE"
BASE COUNT         2830 a         1916 c         2004 g         2616 t
ORIGIN      
        1 tggataacac acaggaataa tatcaaatta atggagttat atttatgaaa ccaaatatga
       61 ttgtaggagc attagcgtta acttctgtgt ttatggcagg tcacctacag gcggctgatg
      121 gaacagtcca tttccgtggt gaaattattg acagtacttg cgaagtcact cctgaaacta
      181 aagatcaggt cgttgactta ggcaaagtaa accgtacagc ctttagtggc gtcgatgatg
      241 tggctgcccc gacggctttt tctatcgatc tgactcaatg cccggaaacc tttaagtccg
      301 ccgcaattcg tttcgatggt aatgaagatg ctcatggtaa tggcaacctg gcaatcggta
      361 ccccgcttga taactctaac gatgctgccg ctggtattag cccgagtgat aacagtgggg
      421 attatactgg tgcgggtgcc gttagtgcag cgaaaggcgt agctattcgt ttatataacc
      481 gtgcagataa cactcaggtc aagttatatg aaaattctgc atcaactccg atttctaatg
      541 gtaatgcatc catgaagttc atggctcgtt atattgctac ggaaacgact attgaccctg
      601 gtacagctaa cgccgactcg cagtttacag ttgaatatat aaaataaatc attcttaaca
      661 taatacctta aataaaatgg cgctcgccat tatggttctc ttttacttaa aagaagggcg
      721 tcattattat atttttatga gatacattta tgcctatgta tttacttaga ttgttttttg
      781 ctgttacttt gattacgcag atgtcgatgg ctatggctgg tattgttgtt gggggaactc
      841 gcgttatttt ttatggtgac aaacctgata ctacaatctc tatttttaat aaagaaacga
      901 accttcctta tctattgcag acatgggttg atccatttaa taaagaggat aaaagcaaac
      961 cgccattttc tgtgatacct cccgtttctc gcattgaacc gcagcaagag aaaatattac
     1021 gcattttaaa aacggctggc gagttaccaa aagatcgaga gtccgtattc tggttgaacg
     1081 tgaaaaacat ccctccttca attgataaaa aaaataccaa tgttcttgag atagccataa
     1141 aaacaagaat taagttattc tggcgaccag ccgcggtaaa gataatccct gagcaggcta
     1201 ttaaaaatgt taaatggtcg cggcagggta ataatctcat cgtcgaaaat ccaaatccga
     1261 tccatattaa tgtaatgagt gtgacggtgg acgataaaga cgtgtcgcta aatatgatcc
     1321 gcccttttga aacattgaca ttaccaattc cggctggcgt ggcaggaaaa tcgctcgttt
     1381 ggcgctttat taatgattac ggtgcaatta gtcaaccact aaagaaaaac ctttaagcgc
     1441 cggataagga tatatcgatg gcctcttcgc atcttttcat tacgcttgca tcgggcatat
     1501 gtctgctctg ttccatatct gcttttgccc gggatagctt gttcaacccc agattactgg
     1561 aactggatca tcctgcggat aatattgata ttcaccagtt caaccgttcg aataccttac
     1621 ctgcgggaac atacaaagtt gatgtgatga tcaacggcat gctcttcgaa cgccaggaag
     1681 ttaaatttgt ccaggataac cctgatgctg aactccaccc atgctacgtg gcgataaaaa
     1741 acgtactggc gacctatggt ataaaagttg atgcgataaa atctctggcg aatgttgatg
     1801 acaaaacatg cgtaaatcca gttccgctga tcgacggggc tacctggtta ctggacgcca
     1861 gtaaacttgc attgaatatt actattccgc aaatttatct caacaatgca gttaatggtt
     1921 atatcagccc ttcccgttgg gatcagggga tcaatgccat gatgatgaat tatgattttt
     1981 cggcatcgca taccatccgg tcaaattatg acgacgacga tgacagttat tatctgaatt
     2041 tgcgtaatgg tattaattta ggcgcatggc gttttcgtaa ttacagcacc ctgaattctt
     2101 atgacggtaa tgtggactac cattccgtca gtaattacat tcagcgcgac atcatggcat
     2161 tacgtagcca gattatgatt ggcgatacct ggacggcaag cgatgtattt gatagtacac
     2221 aggtgcgtgg cgtgcggctg tataccgatg acgatatgtt gccctccagc cagaatggct
     2281 ttgcgccagt ggtacatggg attgcgaaaa ctaacgccac ggtgatcatc aaacaaaacg
     2341 gctacgttat ttatcaatca gccgtaccac agggcgcatt tgccctcacc gacttaaaca
     2401 cgaccagtag cggcggcgat ctcgatgtca ctatcaaaga agaagatggc agcgagcagc
     2461 actttattca gccatttact tcactggcca ttctcaagcg tgaaggtcag accgatgtag
     2521 accttagcat tggagaagtg cgcgacgaaa gcggctttac gcctgaggtc ttgcagttac
     2581 aagcaatgca cggtttccct ttgggaataa ccttgtatgg cggaacacaa ctggcaaatg
     2641 attacgcttc tgccgcgctg ggtattggta aagatatggg ggcgctgggc gcgatttctt
     2701 ttgacgtgac tcatgcccgc tcgcagtttg actacggcga taatgagagt ggtcaatcgt
     2761 atcgttttct ctattccaaa cgttttgaag acaccaatac cacctttcgt ctggtgggtt
     2821 atcgctactc tacggagggg ttctacaccc tcaatgaatg ggtgtcgcga caggataacg
     2881 attctgattt ctgggtaacg ggcaaccgtc gcagccgctt cgaaggcacc tggacgcaat
     2941 ctttcacgcc aggctggggc aatatttatt taacattcag tcgacaggaa tactggcaga
     3001 ccgatgaggt cgagcgttta ttacagttcg gctataacaa caactggcga aacatctcct
     3061 ggaacgtttc ctggaactat acggactcga tcaagcgctc atcgagcaac tatcatgatg
     3121 ataacaatga taatttcggc aaagaacaga ttttcatgtt ctcaatgtcg ataccgctat
     3181 cgggctggat ggaagacagc tacgtcaact attcgttaac gcaaaacaac caccatgaaa
     3241 gcacgatgca ggtcggtctg aacggaacga tgctggaagg gcgtaacctg tcttataacg
     3301 tacaggaatc gtggatgcac tctcctgatg actcctacag cggcaatgcc ggaatgacct
     3361 atgacgggac ttatggctcg gtcaatggca gctattcctg gagccgtgac tcccaacatt
     3421 ttgattatgg cgccagaggc ggcgtgctgg tgcatagtga cggagtgacc ttctcgcagg
     3481 aactgggcga aacggtggca ttagtcaaag cgccgggcgc agaaggcctg tccattgaaa
     3541 acgccaccgg gatttctacc gactggcgtg gttataccgt aaaaacgcag cttagcccat
     3601 atgacgaaaa ccgcgtggca ttgaacagcg actatttctc caaagccaat attgaactgg
     3661 aaaacaccgt catcaacctg gtaccaacgc gcggtgcggt ggtgaaagcc gaatttgtca
     3721 cccatgtcgg ttatcgcgtg ctatttaacg tccgccaggt caacggtaaa ccaataatgt
     3781 ttggcgcgat ggcaaccgcc tctctcgaaa cgggcacagt caccgggatt gtcggtgata
     3841 acggcgaact gtatctctcc gggatgcctg aaaaaggcga gtttttattg agttggggac
     3901 aagctgcgga tgaaaaatgt aaggcggcct atcacatcac ccataaacct gatgatacca
     3961 gcctgattca aatggatgcg atttgtcgct aaaggaagca tgaatgaaaa aaatattatc
     4021 agggttgatt ctgctgcttt gctgtcctta tggtttcgcc gctaatggtg atggcgtttg
     4081 ttttaatagt gatggcgcaa cgcacatgtc aaatttatca tttggtccgc tgacggtggc
     4141 tgcggcgaat aaccactccg gatacaatat tttcgaggca ctgagcaaca cgactggaac
     4201 atacccggtg cgctgtcact gtgatgacac gcatggcgga ccgggccaac aaacagcatt
     4261 ttttcctatc ttctacacgg gggatgccgc accggggctt gtgcttgagc gcactcttaa
     4321 tgggttaaat tactatgctc tgaatgatta tttatcggtc ggcgtgacga tttttattat
     4381 taataaccaa tatgcggcca ttccttttga acacttatcc aaccaatcca cctcaccgca
     4441 acatacctgt ggagcaggta ataatgggag cactgtaaat ctggattcag ggcgctcggc
     4501 aaaattatct ttctatgttc ggcattctat tactggcacg gtgacaatac ccacaacgga
     4561 agtcgcctgg ttatacgcgg gcatgtccga tcattttccc aaaacgaccc ccgtttctaa
     4621 agtgacaatt cgcggacaat taacggcccc gcagaactgt gagttaacgc caaatcagag
     4681 catcgatgtc gattttcaaa aaattaatag cggtgagttc tcctcaacgg cgggttcaat
     4741 tattgcagaa cgaaagatta aaaccgaagt cacagtatcc tgtaccggga tggaagacgt
     4801 aaggtctacg gaggtggtga gtgcgtcgat gattgcggca aacagaagtg ccgatgccac
     4861 catgatcgtg acgagtaatc cggatgtggg aattaagatt tttgataaga acgaccgtcc
     4921 agtgaatgtg gatgggggca acttacccgc tgatatgggt gctattagtc gattaggaaa
     4981 aaccgatggt agcgtaacgt tttattcagc gcccgccagt ctgacgggca caaaaccagc
     5041 gcctgataat ggatttaccg ccacagccac gctggttatt gaatttacta actaatcttg
     5101 tgttagtaaa aggaataatt tcatgaaaaa atggcattat atattttgca taattctctt
     5161 tcatttaggg ttgccgtgcg ggtatgcggc aaatgatggc acgtgtgcaa caagaggcgg
     5221 cacacataca ttaagcctta attttcctct gacaacggtc agtgcagcaa acaatgtgcc
     5281 tggaaataca ttaatagata ttgctaatgc aacatcttct gaaaattata gcgttctgtg
     5341 taactgtgat tcaaaacata gcaatggcgc ttatcacgaa atatattata ccgcagaccc
     5401 tgctcccggt atggtttata gcaccaccgc aagtggtctt gctttttact atcttaacga
     5461 atatgtcgat gtgggaacaa aaatatctgt gctaaatgcg gggtatacgg cagttccttt
     5521 tgaacatgtt tccaaccagg caactacaac agatcacact tgtcagggaa acaaaactac
     5581 agcggttggc gtgagcctga aaactggagc agatgcgaag atttcatttc gtattaaacg
     5641 ttcaataaat ggaacggtag taatacctat caccgatatt gcattgctgt atgccaacat
     5701 atccagcacc acgacccgtg gtgaggcgat tgcaaaagtt cgaatttcag gcagtttgac
     5761 cgcaccacag tcttgtcaga taaatgcagg acaggtgatt tattttgatt ttgatactat
     5821 tcctgcgtcc gaattttcat ctaccgccgg gcaagccatt acttcacgaa aaatcactaa
     5881 aagggggttc gcgcccattt cctctaaaag tctcaccttg cttaggtaat taattgaatt
     5941 tatttataaa acgttgcaat tttagcatca ttttttattg ttctttttaa cctgaagtac
     6001 gtcgatcgag agatacctgt tttttctgtc acactgccca gagattctcc ctgtgatata
     6061 agctccaacg ctgtaggcgt tcgtggatgc ttcttcctgc caaattttac gcctgcggca
     6121 agtgccgcta ttcttccgtc gtttgtccgc tccagaatac gctcacgttc cgcttcagct
     6181 acagcagcaa ggatttggat aaccattttg cccattgtcc cttctgtact gagaccgttc
     6241 tccaggaacc ggatggcgat cccctttttg taacagctat caaccacacg aatcatgtca
     6301 gcagtgttac gccccagtcg gtccattttt gtacagatga ttacgtcgtc tttctctgca
     6361 cgggccaaca accgctgtag tccttcccgt tcatcagttg cgccagtcat catatccgta
     6421 aaaatacgat cttcccgtac cccggcattt tttagctccg agatttgatg tgttagcttc
     6481 tgatgactgg ttgatacccg tgcatacccc aataacgcca tttttagtct cacttatcgt
     6541 ttttgacact gcatggtatc ataatgtaca aaatacgatt tttgaaacct gcttttatgc
     6601 tgttttagac aggtatcaga tatcttaata tttgatacta gccatattta acaaatattt
     6661 agcattttgg taaatgtatg cagaagtaca agaaaagaat tagtatatta acaaaaagtg
     6721 aaataaatga attatatcaa gtcccctctt ttaatcctgt tgaaagggat gagttttttt
     6781 cgctggatat cagccttaaa agagaaattg ataaaataat aaatatagaa agcagggtgt
     6841 atctgatatt aataacaggg tatttcagat ataaacctgt gattcctgaa tttacattaa
     6901 aagacgtaaa aagggatgtg aaatatatta tccagacata ttataaaaac gaaagtacta
     6961 atctcaattt aaacatacct aaaagtacaa agtaccgcct tgttaaacga atgctttcta
     7021 ctctaggatt tgaacaactc accccatcta taaaaaatga gttaagtgaa cgtctgaatg
     7081 atgttgctac tatatgtatg gaaccaaaat atatttttga tgaattactt gcttttttag
     7141 gacagcggcg tatagctctt cctggataca gtactgtcca gaacctcgtt tcagatgctt
     7201 taaactttga aaggaaaagg ataattaata ttttgtcaaa gagaatttat cctgccactg
     7261 cacaaaagat tacatccatc atccatgatg atggaatatt aaattccatc aggggatata
     7321 attatagcac gagagacttc tcagcatcag aaatagaaaa tgaattaaga actcacaatg
     7381 tgataagtag tatttactat gaaataaaag atataattaa ttcattaggt atatcccagg
     7441 gaaacatgga gtactatgca actatcgtaa aacacagtac aacattcaga cttaagcgac
     7501 accctaaatg gcaaggaata ctttatttgt gctgctactt gtacttcaga tacagagaga
     7561 taaatgataa aataatcaca gccttcagat atttagtaaa aaaacataaa gacgcttcag
     7621 ttttgtttgc aaaagctcgg gcaacagagg aaatagatct aataaataaa aagcttaagt
     7681 ttgcgagtaa aatatttaac ttcttcgttg atagtaacat tgaagacagc atcccttttg
     7741 gatatgttcg tgaaaaggtt ttttcactta tatcgaaaga tgatatgcat cttgtgagtg
     7801 aattttttga caaaaataaa atcgatgtaa ttgaatatca atggcagtat attgacagcc
     7861 atcaccttaa gatcgccaat tcaatccgaa agctttttct ggctatagat atagtatatg
     7921 acaacgagaa gtctgttatt ggccagcaaa ttactgctgc caaggatgat ctcagaggaa
     7981 aaggaaagct ggatgctaag gatctgaggg taattcatcc atccgagaaa aattacattg
     8041 taaaagaagg agaaattaac actcaacgat tcgaatttca tatgtacagc agggtatcta
     8101 accatttgga aaacggtgtc ttttatgctg cggagagtga acaaaacaag agactggaag
     8161 atgatctcat agaacgaagt tactggcata agaataaatc gattttgata gagaaaacag
     8221 gccttgaacg acttatcaca ccaataacgc agactcttaa tgagctggag aataccttta
     8281 gcgataaact ggctcgtata accattggaa taaactcaga tgctaatgag tttgtgaaaa
     8341 aacaaccaca ctccaacaga ttacagtgga gtctggccag taaaaaatgg aagaccagca
     8401 tcgataatcc gatttacaat cagataaaaa atatcggaat agttgacgta atgaagtatg
     8461 taaacaatga gacgaatttt ttgtctgtgt ttaatgccat atcatccagg aaaaataatc
     8521 ttgaagctga ctataatgac cttcttgcct gtatatttgg taatggcgca aattatggtg
     8581 ttcacagaat ggcatctgca tcagaccgta gtattggaat actacgtgat gtgaatgaca
     8641 aattcattcg tccagaaaca acaggaatgg ccaacacagt aatatctgat gcaattgcaa
     8701 atctttctgt atataaatac tggacgataa atgaagattc tccatttgga agtgttgatg
     8761 ggcagaagca cacttgcaga ataaatacat ttaaagcccg ttattctgcc aagtatttca
     8821 gaaaggggaa aggggtttct gcgatgacac tggtatctaa tcatgtgcca ttatcaactg
     8881 tggtaatttc tccaaatgaa tatgaaggtc attttgcatt tgacttgtta tataacaaca
     8941 ccagtgaaat acaaccaaag tcattagcga ctgacaatca tggtataaac aacgttaatt
     9001 ttgcgattct tgacattttt ggctatcagt tttcaccacg ttatgcaaaa ttcaagaaaa
     9061 tatttaatga attatttgat gttatactgg agagtgataa actcattata aaacttaaaa
     9121 aacaatttaa ctttaagctc attgaacatg aatgggaaaa catccaacat attatttgtt
     9181 ctttaagcag aaaaacaacc attcaaagta cggttgttaa gaagttatct aatacaaagc
     9241 gtagcaacaa gacattagca gcacttcggg agtatgaccg agttataaaa tgcatatatg
     9301 tcctggatta tgctgacaat aaaaccttaa ggcaatttgt gcaacaagca ctgaacagag
     9361 gtgaag
//
LOCUS       DAZFKF010000157         9224 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFKF010000157 DAZFKF010000000
VERSION     DAZFKF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.159"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(164..955)
                     /gene="cpoB"
                     /locus_tag="ACHKA1_004791"
     CDS             complement(164..955)
                     /gene="cpoB"
                     /locus_tag="ACHKA1_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO5682339.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(965..1486)
                     /gene="pal"
                     /locus_tag="ACHKA1_004792"
     CDS             complement(965..1486)
                     /gene="pal"
                     /locus_tag="ACHKA1_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO5682340.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1521..2813)
                     /gene="tolB"
                     /locus_tag="ACHKA1_004793"
     CDS             complement(1521..2813)
                     /gene="tolB"
                     /locus_tag="ACHKA1_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO5682341.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVFQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(2946..4211)
                     /gene="tolA"
                     /locus_tag="ACHKA1_004794"
     CDS             complement(2946..4211)
                     /gene="tolA"
                     /locus_tag="ACHKA1_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO5682342.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4276..4704)
                     /gene="tolR"
                     /locus_tag="ACHKA1_004795"
     CDS             complement(4276..4704)
                     /gene="tolR"
                     /locus_tag="ACHKA1_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO5682343.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4708..5400)
                     /gene="tolQ"
                     /locus_tag="ACHKA1_004796"
     CDS             complement(4708..5400)
                     /gene="tolQ"
                     /locus_tag="ACHKA1_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO5682344.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5397..5801)
                     /gene="ybgC"
                     /locus_tag="ACHKA1_004797"
     CDS             complement(5397..5801)
                     /gene="ybgC"
                     /locus_tag="ACHKA1_004797"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO5682345.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(5951..6244)
                     /gene="ybgE"
                     /locus_tag="ACHKA1_004798"
     CDS             complement(5951..6244)
                     /gene="ybgE"
                     /locus_tag="ACHKA1_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO5682346.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     DLEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6244..6357)
                     /gene="cydX"
                     /locus_tag="ACHKA1_004799"
     CDS             complement(6244..6357)
                     /gene="cydX"
                     /locus_tag="ACHKA1_004799"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO5682347.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6372..7511)
                     /gene="cydB"
                     /locus_tag="ACHKA1_004800"
     CDS             complement(6372..7511)
                     /gene="cydB"
                     /locus_tag="ACHKA1_004800"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO5682348.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7527..9095)
                     /gene="cydA"
                     /locus_tag="ACHKA1_004801"
     CDS             complement(7527..9095)
                     /gene="cydA"
                     /locus_tag="ACHKA1_004801"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO5682349.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2245 a         2522 c         2219 g         2238 t
ORIGIN      
        1 ctttttcgtt gctttcggtt tgtttgatat cccgtggcaa cggcggcata tattactgat
       61 ttcagacttg agcgcaacaa aaatttcgat gtagatcact caactgctta tgattcgcac
      121 gacacgacca gaaataatgc gacttctggt cgtgtgttat gcattacatc gcgttcagac
      181 gtttttgcgc ctgtttagcg ccatcagtac cagggtattt actgataacc tgctggtata
      241 cggctttcgc ttttgcggtg tcacctttgt cctgcatgat gacgccgact ttaaacatcg
      301 catctgcagc ctttggtgac ttcggatagt ttttcactac cgaagcaaaa tagtacgccg
      361 catcatcttt tttacccttg ttgtagttta actgacccag ccaataattg gcgtttggca
      421 ggtaagttga atcagggtaa tttttgatga aattctgaaa tgccaccatt gcgtcatcct
      481 ggcgggattt atcctgcacc agcgcaatag ctgcattgta atccgtgttt gcatcaccgc
      541 ttttcaccgg cgcgccagca ttcgcagtac cagcatcagc tgtcggcgtc gttgatgccg
      601 ccgcaccgct ttgatcaccg ctggttgatt gcgccgctgc accaccgctg ctgaggctgt
      661 cgatctgcaa caggatctgc ttttgccgct ccacgacctg attcagttga tactgatttt
      721 cctgaatctg accacgcagg gaatcaatat cggattgatt atcagagagt tgttgctgga
      781 gttgggttaa aagctggctg tgagcattag aaatacgctc aagttgagtg acgcggtctt
      841 cgaccgagcc tgagccgaca ctactgattg gtgcctgagc aaaagcggcc cagggggccg
      901 ctataccaac cagtaacgac agactcaata gttgatgtct gaagttactg ctcatgcaat
      961 tctcttagta aaccagtacc gcacgacggt ttttggagta tgccgcttcg tcatgaccca
     1021 gtactgcagg tttttcttta ccgtaagaaa cgatggagat ctggtctgca gaaacgcctt
     1081 taccctgcag gtacatctta acggcgttcg cacgacgttc acccagggag atgttgtatt
     1141 ccggagtacc acgttcgtcc gcgtgacctt ctacggtgac tttgtaagac gggttgctac
     1201 gcaggaagtt tgcatgtgca tccagcattt gagcgaagtc agaacggata tcgtacttgt
     1261 ccagatcgaa gtaaacgatg ttgttctgct gcagctgttg catttgcaga cgagcctgct
     1321 cttcggaaga catgttgccg ttgccgccgt tcgcatccat accagtgccg gcacccagca
     1381 tgccttcgct gccgtcattg ctggcgttct tgttggaaga acatgccgca attgccataa
     1441 caggcagagc aatcatcagc cctttcagca ctttgttcag ttgcatttca atgattcctt
     1501 tactattcaa ttaattatta tcacagatac ggcgaccagg cagggaattt gacctgtcca
     1561 tcagttgccg gaagacgcgc tttgaaacgc ccatctgtag aaaccaaatt cagcacggat
     1621 cccatcccct gagaagagct gtagattacc atagtgccgt taggtgccag acttggcgtt
     1681 tcatccagga acgtggacga cagaacttgt acgcctcccg ttgccagatc ttgtttggca
     1741 atgtgctgct gcccaccgtt ggagctgacc attaccataa atttaccgtc gctgctgaca
     1801 tccgcatcct ggttctgcga accttcccag gtaatacgtt gtggcgcacc gccgttgata
     1861 ttcactttat aaacctgtgg acgaccggcc tggtcagaag taaatgccag gttctggctg
     1921 tccgggaacc aggtcggttc ggtattgtta ctgcgaccat cagtcacctg gcggatctga
     1981 ccagaagcca aatccattac gtacaggttc aggctaccgg ttttcgacaa ggcgaatgcc
     2041 agtttgctgc cgtctggcga gaatgcaggc gcaccgttgt gacgcgggaa tgaagccacc
     2101 tgacgtacag cgccgtttgc cagcgtttgg ataaccagcg cggaacgacc gctttcgaag
     2161 gtcacataag ccagtttaga accgtctggt gaccacgccg gggacatcag cggctgtggt
     2221 gaacggtgaa cgacaaactg gttgtaaccg tcatagtcag atacgcgcag ttcatacggg
     2281 aactgaccgc cgttggtctg aaaaacgtag gcaatacggg tacggaacgc acctttaata
     2341 ccggtcagtt tttcaaacac ttcatcactg gcggtatgac cagcataacg cagccactgc
     2401 ttgttcactt tgtacgagtt ctgagcaagt acagtacccg gtgcgccgcc agtgtcaaca
     2461 agttgataag caacattgta agaaccatcc ggattcggag tgacctgacc gacaactaca
     2521 gcgtcaatac ccagcgcgga ccatgcagct ggttgtactt cctgcgcact acccggctgc
     2581 tgtggcagac gagcgcgatc taacggatta aatttaccgc tgttacgcaa gtcagcagca
     2641 acgataccgc caatatcttc aggtgccgca ccaggacccg cccactggaa aggaacaaca
     2701 ccaataggac gaccggaatc tacaccgctg tcgatcacaa tgcggacttc agcatgcaga
     2761 actgatgccc acagtatgag aaaaccaaat gctactcgta atgcctgctt catcatatct
     2821 cccttatctg gaccgatggc ccacgataat ttagcagaat gttaacaaac taaaatacac
     2881 aaaactacca gaactccgtg gcaaccggtg cctgatgttg accgtcggaa cagtcaacat
     2941 cgcgattacg gtttgaagtc caatggcgcg tttttgaaca cttcatatac tgcctggctt
     3001 ggtggtttcg ggatcttcgc aagtttagct gctgccaacg cagcctgaca aagtgcggga
     3061 tcgccacctt caggtttgat atccagtaac atgccatcgg gtgccagttt tatgcgcagc
     3121 gtacaggttt tgcctgcata ggacgatgcg tcatagaact tactttcgat agcagattta
     3181 atctgcccgg catagttatt gatatcggcc cctgatgcgc cattgttttt agtattacca
     3241 ctcccggcag gcgaagcatt gttccctttc gcccctcccc ccgttttcgg tgcattctta
     3301 ccagagctta gctcaccgaa aatatcatct gcctctgcgg cagcttttgc tgcagcggct
     3361 ttttcagctg ctgcttttgc agctgccgct tttttgtctg cggcggcttt ttctgccgct
     3421 gctttcttat cagctgctgc cttttcagca gccgcttttt ctgctgcttt tttgtcggct
     3481 gcagctttct ctgctgccgc tttcttatca gctgcagcct tttcagcagc cgctttcttc
     3541 tcagcttctg ccttggcttt ttcagcagct tcagttgccg ctttctttct tgcttcagct
     3601 gcagctgctt ctgccgcttc cgctttcttc ttcagtgccg cagcggctgc ctcggctttt
     3661 ttctgcgctt cggctgcggc tttggcggct tctgcttctg cttttttctt cgcgtctgca
     3721 gccgctttct tcgctgcttc ttccgcagct ttagcatccg cttcggcctt cgctttagca
     3781 tctgccgctg ctttcgctgc cgcctcttcc gcttgcttct gctttaactc ggcctgtttt
     3841 gcggcttctt cagcctgctt tttctgttcc tgagcagcta accgctcttt ctcaagttgc
     3901 ttcaggcgtt cctgttcagc cgcttgtttc tcacgcagtt cttcagcagc ctgctgttcc
     3961 ttcatcttgc gctgctcatc agaacgcttc gcgcttgatt cctggctttg catgcgtttg
     4021 tactgctcaa ctaccgcacc tgaatcaacc atgacagcgt cgatggacga accaccgccg
     4081 cctccggctg aagcttctat attctcatcg aacgaactcc agatcagcgc cgcaaataag
     4141 atgacatgca gcactgctga aataattatc gcccgcttga gcttgtcgtt ttgttcggtt
     4201 gcctttgaca ctctcggttt ccaaaaactg ttcgcctgtt acccgctctc tttcaagcaa
     4261 gggaaacgca gatgtttaga taggctgcgt cattaaacca accgatttca cacccgcact
     4321 atgtaacaag ttcagtgctt taattatttc atcgtaaggc acatcttttg cgccaccgat
     4381 cagaaagacc gttttcgggt tggccttgaa acggctggac acttccgcca ccacctgctc
     4441 tggtggtaaa cgctcaaggc gatctttctc aaccaccacg gtgtactgac caataccaga
     4501 cacttcaaca atcactggcg gattatcgtt actgctcacc gcctgtgatt cagtagcgtc
     4561 tggcagatcg acctccacgc tctgggtgat gatgggcgct gtcgccataa agatcagcaa
     4621 cagcaccagc agtacgtcca gcaacggtac aatgttgatt tcggacttga gatcgcgacg
     4681 acctcgtcca cgcgctctgg ccatggctta ccccttgttg ctctcgctaa cggtaaacgc
     4741 ctggcggtgc agaatcgcgg taaactcttc cataaagttg tcgtaattca gttccagttt
     4801 gtttacgcgc tggttgagac ggttataagc cattaccgcc ggaatagcgg cgaacagacc
     4861 gattgctgta gcaatcaacg cttctgcgat gcctggcgca accatttgca gtgttgcttg
     4921 ttttaccgcc ccgagggcga taaaggcgtg catgatcccc cagaccgtac caaacagacc
     4981 aatatacggg ctgatggagc caaccgtacc gaggaacgga atgtgcgttt ccagattttc
     5041 aagttcacgg ttcatggaaa tacgcatcgc acgcgacgcc ccttccacta cggcttccgg
     5101 cgcatggcta ttggcacgat gcaggcgcac aaattctttg aacccgctgt aaaagatttg
     5161 ttccgaaccg gtcagattat cccgtttccc ctggctctct tgatagaggc gagagagttc
     5221 gattccagac cagaatttat cctcaaacgc ttcggcttcg cgcgccgctg cgttaagaat
     5281 acgggtccgc tggataataa tggcccagga tgcgattgaa aaaccaatca aaatcaacat
     5341 gataagttta accagaaggc tagccttcag gaacaaatca aggatattca tgtcagtcac
     5401 tgcttaaact ccgcgacaat agacttggga agcgcacgag gcttcatttt gagtgggtca
     5461 acgcaaacaa ccagaacctc tgcttcattc agcaaagtat tctcggcgtt gacaatacgt
     5521 tgcgtgaaaa ccaaagaggt gccacgcatt gatgttattt cagtctgtat ttcgagcata
     5581 tcgtcgagcc gcgcaggtgc gtaatattcc accgtcattt tacgtaccac aaaggcaacg
     5641 cgttcagcca tcagcgcctg ctgactgaag tgatgatgac gcagcatctc tgtgcgtgct
     5701 ctttcataaa aagcgacgta actggcgtgg tacaccacac cgccggcatc ggtatcttca
     5761 tagtagacgc gaaccggcca tcgaaacagc gttgtattca ctttacatcc cggtaatgca
     5821 acaaaagtta gagcttttaa acgctgctac tatacgcgcg cgattgaggt ttgggaatga
     5881 gggcaagtta agggagcgta aaaatttatg ggcctggcgc ggcccataat tttttaaaga
     5941 gaattctgat ttaaaagaag aagaaaatca gcccgacaat gagaacaata tcggcaagca
     6001 atgggcaaaa aatcccttgc caaagaacct tctgcggacg aaagcccacg ccgtgaatca
     6061 caccagcaca gacggcccac atcagcaata aaccatgcca gatttccaga tcactggtct
     6121 tcgcggcgaa acgtgatggg tcccaaaaca tacatcctgc taacagaagc gccatcacga
     6181 aggaaagcgc ccgcaggggg cgcttgtcca ttaccgcata caaagtcgcg ataatcttac
     6241 tcatcagatg tcttcttgac cggctttgcc tgattcgacg tgctcaagcg ccagcgcggt
     6301 gattacccca aacgaacagg caagaagcgt tcccagaatc catgcgaaat accacatttt
     6361 tagctcctta cttagtacag agagtgggtg ttacgttcaa tatcttcttt ggtgatacga
     6421 ccgaacattt tccagtaaca ccaggcggtg tagagcagaa tgatcggtac cagaaccacc
     6481 gcaacccagg tcatgacgtt aagcgtcagc tggctggaag ttgcatccca cattgtcagg
     6541 cttgcgttca tcatggtgct ggacggcatc acaaacggga acattgcaat accagcagtc
     6601 aggatgatgc aggccagcgt cagggaggag aacacaaacg cccatgcggc tttatccata
     6661 cgtgcagtca ggatggtcag cagcggcaga accacaccca gtgccggaat agcccacaga
     6721 attggcgtgt tgttgaagtt aaccagccat gcgccagctt cacgaaccac ttctttattc
     6781 agcgggttag aggctgcgta atggtccatt gtcgatttca cgacataacc atcgataccg
     6841 tacatcaccc atacgccagc cagtgcgaaa cagaccagtg tcaccagtgc cgccacctga
     6901 gccgttgcac gggtacgcag gtgcagttcg cccacggtac gcatttgcag ataggttgcg
     6961 ccctgagtaa tgatcatccc tacgctcacc acgcctgcca gcaggccgaa cgggttaagc
     7021 agctggaaga agttaccggt gtagtacaga cgcagatatt catcaacgtt gaacggtacg
     7081 ccctgcaaca ggttaccgaa cgctacacca atcaccaacg gcggaacgaa gctaccaatg
     7141 aagatgcccc agtcccacat gttacgccag cgggtttctt caatcttgga gcggtagtca
     7201 aaaccgaccg gacggaagaa caaagacgcc agcacgagga tcatcgccac atagaagccg
     7261 gaaaacgcag cggcatagac catcggccag gcagcaaaca gtgcgccgcc tgcggtgatc
     7321 agccaaacct ggttaccgtc ccagtgtggt gcgatggagt taatcataat tcgacgctcg
     7381 gtgtcgttac gaccgaggaa acgggtgagc atgcccaccc ccatgtcgaa accatcagtg
     7441 actgcaaaac caatcagcag aacgccaacc agcagccacc agataaaacg caatacttca
     7501 taatcgatca tttgacgact cctgtcttag cgtgccggct gagtagtcgt ggaagactgc
     7561 tcaaagtgat agcgaccggt tttcaggctg cttgggccga ggcgtgcaaa cttgaacatt
     7621 aagaacaatt ctgccaccag gaacagggta tacaggccgc aaatcagcac cattgagaag
     7681 atgagatcgc ctgcggtcag tgacgagttc gccacagctg tcggcagcac ttcaccgata
     7741 gcccacggtt ggcggccata ttcagccacg aaccagcccg cttctacagc aatccacggc
     7801 agcggaatac cgtacagcgc ggcgcgcagc agccattttt tctcgccaat gcggttgcgg
     7861 ataacactcc agaaagagag cgcgatgatt gccagcagca ggaagccaca cgccaccatg
     7921 atacggaacg cgaagtacag cggcgctaca cgcggaatgg agtctttggt tgcctgttga
     7981 atctgagctt cagtcgcatc agccacgttt ggcgtatagc gtttcagcag cagaccataa
     8041 ccgaggtctt tcttcatgct attgaactgg tcacgaaccg cctggtcggt agaaccagaa
     8101 cgcagctgtt cgagcagaga gtacgccttc atcccgttac gaatgcgttc ttcatgctgc
     8161 accatcagtt ctttcaggcc gataactgga gtatccacgg aacgcgttgc aatgatgccc
     8221 agcgcgtaag ggatctggat cgcaaatttg ttcgtctcct cttcctgatc aggaatgccg
     8281 aacagagtaa aggcagcagg cgcaggttgc gtttcccact cggcttcaat agcagccagt
     8341 ttggttttct gcacgtcgcc catttcgtag ccggattcat cacccagaac aataacagac
     8401 agaacggcag ccataccgaa gctggcagcg atagcaaagg agcgtttagc gaaggcgaag
     8461 tcacgacctt tcagcatata ccatgcgctg ataccgagga tgaacatcgc gccagtaaca
     8521 taaccagacg ctacagtgtg aacgaatttc acctgagcaa ccgggttaag caccagctcg
     8581 gagaagctca ccatctccat acgcatagtt tcaaagttga aatcggacgc gattgggttt
     8641 tgcatccagc cgtttgcaac cagaatccac agtgcggaca ggtttgaacc gagcgccacc
     8701 agccaggtga cacacatatg ctgaacttta cccagacgat cccaaccgaa gaagaacaga
     8761 cctacaaagg tggattcgag gaagaaggcc atcagacctt cgattgccag cggcgcaccg
     8821 aagatatccc ctacatagtg ggaatagtaa gaccagttag tcccgaactg gaactccatg
     8881 gtcagaccgg tagccacacc cagagcgaag ttgataccaa acaacttgcc ccagaacttg
     8941 gtcatatctt tataaatctg tttgccggaa aggacgtaga ccgtttccat aatggccagc
     9001 aggaacgcca taccgagcgt cagtggcaca aaaaggaagt ggtacatcgc ggtcaaggca
     9061 aactgtaagc gcgacagttc gactatatct aacatcatga ctccttgctc atcgcatgaa
     9121 gactccgaga gtgaaccccg ttagaaaggg tcacacgcat gccccaatac aaaattattt
     9181 gctcgcctct atcgttgcca tttcatctaa agaggaaaag acaa
//
LOCUS       DAZFKF010000158         9191 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFKF010000158 DAZFKF010000000
VERSION     DAZFKF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.160"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..429
                     /locus_tag="ACHKA1_004802"
     CDS             <1..429
                     /locus_tag="ACHKA1_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5682350.1"
                     /translation="PISNKRGQLDLDAWRDFYISYLRRSKNDVPDGDSEDDYEEKLLI
                     ARWELTAEQAVTQQLKNEVSKGKLIDTGFCIFALSKLAMALSSTLDSIPLSMQRQFPD
                     LTPRHLDHLKTLIAKGANQCARAGDKLPDLLDEYIRATTE"
     gene            401..2329
                     /locus_tag="ACHKA1_004803"
     CDS             401..2329
                     /locus_tag="ACHKA1_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5682351.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYTKMLLGVAGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVESTIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAAGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            2313..2519
                     /locus_tag="ACHKA1_004804"
     CDS             2313..2519
                     /locus_tag="ACHKA1_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO5682352.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2516..4108
                     /locus_tag="ACHKA1_004805"
     CDS             2516..4108
                     /locus_tag="ACHKA1_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5682353.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPSQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPSMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQQDNPLINILEKFSSWYDTNHVTLGGVKIPHLFPGD
                     GLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIDSGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            4098..5603
                     /locus_tag="ACHKA1_004806"
     CDS             4098..5603
                     /locus_tag="ACHKA1_004806"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO5682354.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSLPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTSVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPSEVRQDMQQRIDAAHRMFAEKVAMYTGLSVEAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAALNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPD
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKRAEAQAEKLAAFPGMTVEAARDILS
                     SSPDKAEPVSASTTAMFERIMANHSPAAVQGGVSQTSADGDADVKMLMAMP"
     gene            5640..5987
                     /locus_tag="ACHKA1_004807"
     CDS             5640..5987
                     /locus_tag="ACHKA1_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO5682355.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISQATPALTPLMLD
                     EASGKLVVWDGQKAGSAAGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFVGTALSHAALP"
     gene            6045..7073
                     /locus_tag="ACHKA1_004808"
     CDS             6045..7073
                     /locus_tag="ACHKA1_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5682356.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPTYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKN
                     NIIQGSGKEWSKQDRDTFDPTYDIDLFCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGSVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWMTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7126..7494
                     /gene="gpFI"
                     /locus_tag="ACHKA1_004809"
     CDS             7126..7494
                     /gene="gpFI"
                     /locus_tag="ACHKA1_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO5682357.1"
                     /translation="MATKEENQKRLRELAGLLGREADMSGSAADIAQRVAEWEEEVCA
                     SENEIADVDDTVYEQAYRNTGEDALGILERIRLLKCFYLCGVDDETGEPVEHVDAGRV
                     ILMPPSVAKDMVRSGMAVYA"
     gene            7487..7840
                     /locus_tag="ACHKA1_004810"
     CDS             7487..7840
                     /locus_tag="ACHKA1_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO5682358.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGIRAQFTSGAQRGGEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            7856..8434
                     /locus_tag="ACHKA1_004811"
     CDS             7856..8434
                     /locus_tag="ACHKA1_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5682359.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMAINRVATTAINQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQVRIKVNRGDLPVIRLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPEELAYALKQQLRIAIKR"
     gene            8431..8826
                     /locus_tag="ACHKA1_004812"
     CDS             8431..8826
                     /locus_tag="ACHKA1_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO5682360.1"
                     /translation="MKHTDIRAAVLDALEKHDTGATLFDGRPAVFEEADFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMAAIPALAGMITTM
                     VTQGYEYHRDDDMALWSSADLTYSITYEM"
     gene            8834..>9191
                     /locus_tag="ACHKA1_004813"
     CDS             8834..>9191
                     /locus_tag="ACHKA1_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO5682361.1"
                     /translation="MATPNPLAPVKGSGTTLWVYTGKGDAYANPLSDDEWTRLAKIKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDV"
BASE COUNT         2143 a         2172 c         2859 g         2017 t
ORIGIN      
        1 ccgatatcga ataagcgagg gcaactggat ctggatgcct ggcgcgattt ttacatatcg
       61 tatctgagga gaagtaaaaa cgatgtgcct gatggcgata gcgaagacga ctatgaggag
      121 aaattgctta ttgccagatg ggaactgaca gcggaacagg ctgttacaca gcagttaaaa
      181 aatgaggtgt caaaaggaaa acttattgac accgggttct gtatttttgc cctcagtaag
      241 ctggcaatgg cgttatccag tacgcttgat tccatccctt tatccatgca gcgacagttt
      301 cctgatttaa caccgcgcca tcttgaccat ctgaaaaccc ttattgcgaa gggggcaaat
      361 cagtgtgcgc gggcggggga taaattaccg gatttactcg atgaatatat cagagcaaca
      421 actgaataat atgatgagcg ctgtcacaac agcattacag cccctgataa gggcattgcc
      481 ggtgacgcca gttgaatggg ctgatcaaaa ttattatctg cctaaagaat cttcatatgg
      541 tgagggagaa tggaaaacgc tgccgttcca gatcgccatc atgaacagta tggggaatga
      601 tcagatccgc actgttaatc tgattaaatc tgcccgtgtt ggctatacaa agatgttgct
      661 gggagtcgcc gggtatttta ttgagcataa atcccgaaac agtctgcttt ttcagcctac
      721 ggattctgcc gctgaagatt ttatgaaatc tcacgtggaa tccacgattc gcgatgttcc
      781 ctgcctgaaa aaactttctc cctggctggg acgtaaacat cgtgataata ccctcacgct
      841 gaaacgcttt tcatcgggtg tgggtttctg gtgcctgggt ggtgcggcag caaaaaacta
      901 ccgtgaaaaa tctgtggatg tggtctgcta tgacgaactt tcctcgttcg agccggatgt
      961 tgaaaaagag ggctcgccaa ccctgctggg ggataaacgt attgagggct cggtatggcc
     1021 aaaatccatt cgcggctcga cgcctaaaat aaaaggttcc tgccagatcg aaaaagcggc
     1081 caacgagtcg gcgcatttca tgcgttttta tgtgccctgc ccgcactgtg gggaggagca
     1141 gtatctgaaa tttggcgatg agtccacgcc ttttgggctt aaatgggaga aggacagccc
     1201 cgaaagcgtt ttctacctct gtgaacatca tggctgcgtg atccatcagt ctgagcttga
     1261 ccagagcaac gggcggtgga tctgtgaaaa cacggggatg tggacccgtg atggtctgac
     1321 gtttttcagc gctgcgggta atgaaattcc gccgccgcgc tccatcacgt tccatatctg
     1381 gacggcgtac agtccgttca ccacctgggt acagatagtc tatgactggc tggatgcact
     1441 gaaagatccc aacggcgtga aaacctttgt gaacaccacg ctgggcgaga cctgggaaga
     1501 ggccgtgggc gaaaaactcg atcaccaggt actgatggat aaggttgtgc gttacacggc
     1561 ggcggtgcct gcccgggtgg tttatctgac ggcgggcatt gactcgcagc gaaaccgttt
     1621 tgagatgtat gtctggggat gggctccggg agaggaagcc tttctggtgg ataaaatcat
     1681 cattatgggg cgtcctgatg aggaagagac gctgttacgt gtggatgcgg cgatcaacaa
     1741 aaaataccgc catgcagacg gaaccgaaat gaccatttcc cgggtctgct gggacaccgg
     1801 ggggatcgat ggcgaaatcg tttatcagcg gtcaaaaaaa cacggtgttt tccgggtgct
     1861 gccggtaaaa ggcgcatctg tctatggcaa gccggtgatc accatgccaa aaacccgcaa
     1921 tcagcggggc gtgtatctgt gtgaagtggg gacggacact gcaaaagaaa ttctctatgc
     1981 ccgtatgaaa gccgatccca cgcctgcgga tgaggccacg tcgtatgcca tccgttttcc
     2041 tgatgatccg gagatttttt cgcagacaga ggcgcagcaa ctggtggcgg aagagctggt
     2101 ggagaagtgg gaaaaaggaa agatgcgtct gctgtgggat aacaaaaagc ggcgtaacga
     2161 agcgctggac tgcctggtgt atgcctacgc ggcattacgt gtgtccgtgc aacgctggca
     2221 gcttgatctg gctgtactgg caaaatcccg ggaagaagag acgacccggc caaccctgaa
     2281 agaactggca gcgaagctgt ccggaggagt gaatggttac agtcgctgaa ctgcaggcgc
     2341 tgcgtcaggc gcgccttgat ttattaaccg gtaaacgggt ggtgtctgtc cagaaagatg
     2401 gtcgcagaat tgaatatacg gcggcttctc tggatgagct taaccgtgcg atcaatgatg
     2461 cggagtcggt actggggaca acccgccgtc gccgtcgtcc gctgggagtg aggttatgaa
     2521 acgaacgcct gtcctgattg atgtgaacgg cgttccgctt cgggagagtc tcagctacaa
     2581 cgggggcggt gcaggatttg gcgggcaaat ggcggagtgg ttgccaccct cgcagagtgc
     2641 cgatgcggcc ctgctgcccg cgttgcgtct ggggaatgcc cgtgcagatg atctggtgcg
     2701 caataacggg atagcggcta atgcggtggc cctgcataaa gatcatattg tcgggcatat
     2761 gtttctgatc agctaccgtc cgaactggcg ctggctgggg atgcgggaga ctgcggcaaa
     2821 aagttttgtc gatgaggtgg aggcggcctg gtcggaatac gccgaaggga tgtctggcga
     2881 gatcgacgtg gaaggaaaac gcacgttcac ggaatttatc cgtgaaggtg tgggcgttca
     2941 tgcgtttaac ggcgaaatct ttgtgcagcc ggtctgggat acggagagca cgcagctgtt
     3001 tcgtacacgt tttaaagccg tgagtccgaa acgggtggac acgccaggac acggtatggg
     3061 gaaccgtttt ctgcgggccg gtgtggaggt cgatcgatat ggccgtgccg ttgcgtacca
     3121 tatctgtgag gatgattttc cgttctctgg tagtggacga tgggaacgga tcccgcgtga
     3181 acttcccacc gggcgtccgt ccatgctgca tattttcgag ccggtggagg acgggcagac
     3241 ccgtggggcc aatcagtttt acagtgtcat ggaacggctg aagatgcttg attccctgca
     3301 ggcaacacag cttcagtcgg ccattgttaa agccatgtat gcagcgacga ttgaaagtga
     3361 acttgatacc gaaaaggcct ttgaatatat cgcgggggca ccgcagggtc agcaggataa
     3421 tccgcttatt aatattctgg agaagttctc cagctggtat gacacgaatc atgtgacgct
     3481 gggcggtgtc aaaattccgc accttttccc cggtgatggt ctgaaacttc agaccgcgca
     3541 ggattcagac aatggatttt cggcgcttga acaggcgctg ctgcggtata tcgccgccgg
     3601 tcttggcgtt tcctacgaac agttgtcccg tgattactcg aaggtcagtt attcaagtgc
     3661 ccgcgcctcc gccaatgagt cgtggcgcta ttttatggga cgacgaaaat ttattgcggc
     3721 ccggctggcc acgcagatgt tttcctgctg gctggaagag gcacttctgc gggggattat
     3781 tcgtcctcca cgggcgcgtt ttgattttta tcaggcgcgt tcagcctggt cacgggcaga
     3841 gtggattggc gcaggaagaa tggccattga cgggctcaag gaggtccagg aatcggtgat
     3901 gcgtattgat tccggactga gcacgtatga gaaagagctg gcgctgatgg gcgaggatta
     3961 tcaggacatt ttccgccagc aggtcaggga atccgcagag cgggaaaaag ccggactctc
     4021 acgtccggtg tggatagcgc aggcgtatca gcagcagata gcggagagcc gcaggccgga
     4081 agaggagaca acaccacgtg agacgtaatc tttcacacat tattgccgca gcattcaatg
     4141 aaccgctgct tctggagcct gcctatgcgc gggttttctt ttgcgcgctc gggcgcgaga
     4201 tgggggcagc aagtctttcg ttaccacaac agcaggtaca gcttgatgct cccgggatgc
     4261 tggctgaaac ggacgagtac atggccggag gtaaacgacc ggcccgtgtt taccgggtgg
     4321 tgaacggtat tgcggtactg ccggtgaccg gcacgctggt gcaccggctg ggtggtatgc
     4381 ggccattttc cggaatgaca ggctatgacg gcattgttgc ctgtcttcag caggcaatgg
     4441 cagacacctc tgtccggggc gtactgctgg acattgacag tccgggcggg caggccgccg
     4501 gtgcgtttga ctgcgctgac atgatttacc gcctccggca gcagaagccg gtctgggcac
     4561 tgtgtaatga cacggcctgt tctgcggcca tgctgctggc gtcggcctgc tcccgacggc
     4621 tggttaccca gacatcccgt atcggctcca ttggcgtgat gatgagccat gtcagctatg
     4681 ccggtcatct ggcgcaggcc ggagtggata tcacgctgat ttatgccggg gcgcataagg
     4741 tggatggcaa tcagtttgaa gcgttgccgt cagaggttcg ccaggacatg cagcagcgga
     4801 ttgatgcagc gcaccggatg tttgccgaaa aagtggcgat gtatacgggg ctgtctgtgg
     4861 aagctgtcac ggggacagag gctgccgttt ttgaaggtca gtccggtatt gaggccgggc
     4921 tggcggatga attaatcaat gcgtcggatg ccatcagcgt gatggctgcg gcgctgaata
     4981 cacatgatac aggaggcact atgccgcaat taactgcaac ggaagccgcc gcgcaggaga
     5041 accagcgagt gatggggatc ctgacgtgtc aggaagcgaa aggacgtgaa cagcttgcca
     5101 cgatgctggc aggacaacag ggcatgagcg ttgaacaggc ccgggcgatt ctggccgcgg
     5161 cagcaccgca gcagccggtg gcatccgcgc agagtgaagc cgatcgcatt atggcgtgtg
     5221 aagaagctaa aggtcgtgaa caactggcgg caacgctggc ggcgatgccg gatatgacgg
     5281 tggaaaaagc ccgcccgatc ctggcggctt caccgcaggc ggatgccgga ccctcactcc
     5341 gtgatcagat tatggctctg gatgaggcaa aaagggctga ggcgcaggct gaaaaactgg
     5401 cggcgtttcc cggaatgacg gtggaggctg cccgcgacat tctgtcctca tcgccggata
     5461 aagcagaacc ggtttctgca tccacaaccg ccatgtttga acgcatcatg gcgaaccatt
     5521 caccggcagc cgtgcagggt ggcgtgtcac agacgtcagc agacggtgat gcggacgtga
     5581 aaatgctcat ggccatgcca tgaagtcagt gctgaccatc aatatgaggt ttttacaata
     5641 tggtaacgaa aaccatcact gaacaacgtg cagaagtacg tatttttgcc ggtaatgatc
     5701 cggctcatac cgccacaggc agcagcggga tttctcaggc aacaccggca ctgacgcccc
     5761 tgatgctgga tgaagccagc gggaaactgg tggtctggga cggacagaaa gccggtagtg
     5821 cggctggcat actggtactg ccgcttgaag gcacagagac ggtgctgacg tattacaagt
     5881 cggggacctt tgcgacggag gcaatccgct ggcctgaaag tgtggatgaa cacaaaaagg
     5941 ccaacgcctt tgtcggcaca gccctgagtc acgcggcgct gccgtaacac gttatcaggc
     6001 caccgcgttg gcctgactga tttctgaatg aaaggaactg atttatggga ttgtttacga
     6061 cccgccagtt actcggttat accgaacaaa aagtgaaatt tcgtgcgctg tttctggaac
     6121 tgtttttccg ccgtacggtg aatttccaca ccgaagaggt gatgctggac aaaattaccg
     6181 gaaaaacgcc ggtggcagcc tatgtctccc cggttgttga aggaaaagtg ctgcgtcatc
     6241 gtggtggtga aacccgcgtg ttgcgtccgg gctacgtcaa gccgaaacac gaatttaatt
     6301 accagcaggc ggtggagcgc cttcccggtg aagatccggc gcagctgaac gacccgacct
     6361 accgtcgtct gcgtatcatc accgataacc tcaaacagga agagcacgcc attgtccagg
     6421 tggaggaaat gcaggcggtg aatgccgtgc tgtatggcaa atacaccatg gaaggagacc
     6481 agttcgagaa aattgaggtt gattttggca gatcgacgaa gaataacatc atacagggta
     6541 gcggtaagga gtggtcaaaa caggaccgtg acacgttcga cccgacatat gatatcgacc
     6601 ttttctgtga tcaggccagc ggtcttgtga atattgccat catggacggt accgtctggc
     6661 gtctgctgaa tggctttaag ctgttccgcg aaaaactgga tacccgtcgc ggctcaaatt
     6721 cacaactcga aacggcagtg aaagacctgg ggtcggtggt gtctttcaag gggtattacg
     6781 gcgatctggc cattgtggtg gcgaaaacgt cttatgtggc agaggacggt accgaaaaac
     6841 gttatctgcc ggagggcacg ctggtcctgg gaaatacggc tgctgagggc atccgttgct
     6901 atggtgccat tcaggatgcg caggcgttgt ccgaaggtgt ggtggcctct tcccgttatc
     6961 cgaaacactg gatgaccgtg ggcgatccgg cccgtgaatt caccatgacg cagtccgctc
     7021 cgctgatggt gctgccggat ccggatgagt ttgtggtggt acaggtgaaa taatccgtga
     7081 gcgggggcga aatgcccccg tgtctttttt cacagggggc tggatatggc aacaaaagaa
     7141 gaaaatcaga aacgtcttcg tgaactggct ggcctgctgg ggcgcgaggc ggatatgtcg
     7201 gggagtgcag cggatatcgc acagcgtgtg gcagagtggg aagaggaggt ttgcgcatcg
     7261 gaaaatgaaa tcgcagatgt tgatgatacc gtttatgagc aggcatacag gaacaccggt
     7321 gaggatgcct taggtattct ggaacgtatc aggcttctga agtgttttta cctgtgcggc
     7381 gttgacgatg aaacaggtga gcctgttgag catgttgatg ctggcagagt aattctgatg
     7441 cccccctcag tggcaaaaga catggtcagg agtggaatgg ccgtttatgc gtgattttca
     7501 gaatgccttt gatgctgccc tcgccggggt ggacagtacg atcgttgaag tgatggggat
     7561 ccgtgcgcag ttcacctccg gtgcacagcg tggcggcgaa gttcaggggg tttttgacga
     7621 tccggagtcg ctgggttttg ccggtggcgg ggtccgtatt gaaggaagca gcccgtcatt
     7681 atttgtgcgg acggatacgg tgcgtgctgt gcggcgtggt gacacgctga ccattaacgg
     7741 cgagatgttc tgggtggatc gtgtttctcc ggatgacggg ggcagctgtt atctctggct
     7801 caaccgtggg caaccacccg ctgttaaccg gcgacgataa acgcagggtg aaattatggc
     7861 gataaaaggg cttgatcagg cgattgataa tctgagccgg gttcgtaaaa acgccattcc
     7921 tgctgcttca gcaatggcca ttaaccgcgt ggccacaacg gcaataaatc agtctgcgtc
     7981 acaggttgcc cgtgagacaa aggtacgccg gaaactggta aaggaaaggg ccaggctgaa
     8041 aagggccacg gtaaaaaatc cgcaggtcag aatcaaagtt aaccgggggg atttgcccgt
     8101 aatcaggctg ggtaatgcgc gggttgttct gtcccgacgc aggcgacgta aaaaggggca
     8161 gcgttcatcc ctgaaaggtg gcggcagtgt gcttgtggtg ggtaaccgtc gcattcccgg
     8221 cgcgtttatt cagcaactga aaaatgggcg gtggcatgtc atgcagcgtg tggccgggaa
     8281 aaaccgttac cccattgatg tggtaaaaat cccgatggcg gtgccgctga ccacagcatt
     8341 taaacagaat attgaacgta tccggcgtga acgcctgcca gaagaactgg catacgcgct
     8401 gaaacaacaa ctgaggattg cgataaaacg atgaaacata ctgatatccg tgcagccgta
     8461 ctggatgcac tggagaagca tgacaccggg gcgacgctgt ttgatggtcg ccccgctgtt
     8521 tttgaggagg cggattttcc ggcggtcgcg gtttatctga cggatgcaga gtataccggt
     8581 gaagagctgg atgctgatac ctggcgggcc acactgcata ttgaggtgtt tttaccggca
     8641 caggtaccgg attcagagct tgatcagtgg atggaaagcc ggatttaccc ggcgatggcg
     8701 gcgatccctg cactggcagg catgattacc acgatggtta cgcagggcta tgagtatcat
     8761 cgtgatgacg atatggcatt gtggagctct gcagatctga cttattccat tacatacgag
     8821 atgtgaggat gatatggcaa caccaaatcc cctggcgccg gtaaaaggtt ccggcaccac
     8881 gctctgggtt tacaccggta agggcgatgc ttatgcaaac ccgctgtcag atgatgagtg
     8941 gacgcgtctg gcaaaaataa aggatctgac ccccggcgag atgacggcgg aatcctacga
     9001 cgataactat ctggatgatg aggatgccga ctgggtatcc accgggcagg ggcagaaatc
     9061 cgccggtgac accagtttta cgctggcctg gaagcccggc gagaaagggc agcgtgattt
     9121 gattgcctgg tttgacagca gcgagacccg ggcctacaaa atccgcttcc cgaacggcac
     9181 ggtggatgtg t
//
LOCUS       DAZFKF010000159         8861 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFKF010000159 DAZFKF010000000
VERSION     DAZFKF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.161"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(206..1306)
                     /gene="chaA"
                     /locus_tag="ACHKA1_004814"
     CDS             complement(206..1306)
                     /gene="chaA"
                     /locus_tag="ACHKA1_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO5682362.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1576..1806
                     /gene="chaB"
                     /locus_tag="ACHKA1_004815"
     CDS             1576..1806
                     /gene="chaB"
                     /locus_tag="ACHKA1_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO5682363.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            1964..2659
                     /gene="chaC"
                     /locus_tag="ACHKA1_004816"
     CDS             1964..2659
                     /gene="chaC"
                     /locus_tag="ACHKA1_004816"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO5682364.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTALQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2703..3056)
                     /gene="ychN"
                     /locus_tag="ACHKA1_004817"
     CDS             complement(2703..3056)
                     /gene="ychN"
                     /locus_tag="ACHKA1_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO5682365.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3242..4636
                     /gene="ychO"
                     /locus_tag="ACHKA1_004818"
                     /gene_synonym="iatA"
     CDS             3242..4636
                     /gene="ychO"
                     /locus_tag="ACHKA1_004818"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO5682366.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4637..5287)
                     /gene="narL"
                     /locus_tag="ACHKA1_004819"
     CDS             complement(4637..5287)
                     /gene="narL"
                     /locus_tag="ACHKA1_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO5682367.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5280..7076)
                     /gene="narX"
                     /locus_tag="ACHKA1_004820"
     CDS             complement(5280..7076)
                     /gene="narX"
                     /locus_tag="ACHKA1_004820"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO5682368.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7415..8806
                     /gene="narK"
                     /locus_tag="ACHKA1_004821"
     CDS             7415..8806
                     /gene="narK"
                     /locus_tag="ACHKA1_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO5682369.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         2153 a         2162 c         2297 g         2249 t
ORIGIN      
        1 ctcaattcat cttcacttca tccctgaaat gtttgcaatg aagagtacat tccggctttt
       61 caacagctgt tgcagtggtt tcgtgcaggt tgtggtacag gctcgcaatt ctgattaacg
      121 acctgtccag gtagtatgaa gaaatacaat aagcaggaat ttatattatc tcccccgatt
      181 tccgtcatcg gaggagatat aactgtcagg caaatatcgt catcaaatag gcggcaaaca
      241 gtgccagatg cgctgcgcca ttgagcacgt tagtacgtcc ggtggagaag gagatatggc
      301 acagcactaa agaggccacc atcaccacca tttctggcgc accaagtgca aactgcaatt
      361 cgttacccgt cataaatgca attagcgtga cgacaggtac ggtaagcgaa attgttgcta
      421 acactgaacc aaagaacaga ttcatcgcgc gctggacctg gttgttcaac actgctttta
      481 atgcacctaa accttccggc gacagaatca acagtgccac caggaagcca gtaaaggcga
      541 caggggcatt catgctgtcg agcaaagtct ccagcgagct ggcgttcatt ttggtcaccg
      601 caataacggc aatcagatgg ataatcaacc agatagcatg ccacaggctg ctatgggcag
      661 acggtttacc gtggtgcggg tcgtcgtcat cactgtcatc ttcgtgctcg tagacaaaca
      721 aactttgatg cgttttggtc tggatcagca aaaatacgcc atacattgcc gcagaaatta
      781 atgctaccag taacgcctga ccggttgaaa aattcgccgc aggcagagcc attggaaata
      841 ccagtacgat tatcgccagg gggaacagcg caattaaata ctgcttgata ccaaacagat
      901 tcatatattg ggtggcaaac ttacgaccgc ccaacaataa tgaaaagcca accagcccac
      961 cggtaacaat cataatgatt gaatagagcg tatcacgcat tagcgttggc gcggcgtcgc
     1021 cggttgccat taaagctgaa atcaaactga cttcaagaat aaccactgaa aggctaagaa
     1081 taagcgaacc gtaaggttct cccaggcgat gggctaatac gtccgcatga cggacaacac
     1141 taaaggcgct acttaaaata ccaataagcg caagaagatt gatggcaatg accactggta
     1201 gtgtctggct gcttccccac aggaacagca ctaccagcgc cagaaccggg aaaataagcg
     1261 aagtctcctt gtggcgggtt tttaccgcct cttgagcatt tgacattatg gttatccctt
     1321 tgcagatgaa tttgtcgaaa atgtaaaaaa taggtaggaa aaataacaga aagtgtctgg
     1381 atatcggtaa cattttacga aattttaccc cgctgtcgat tttttactca ttggggcata
     1441 aaaataagta atacgtttag acaatgtttg ttttagcgtt tcttaagaac agtctgacat
     1501 gaaaattctt atgttttggc aagagtagat attgttgacc acacttaatg ttcaactttg
     1561 taaaaggagt caacgatgcc gtataaaacg aaaagcgacc tgccggaaag cgtaaagcac
     1621 gttctaccgt ctcatgccca ggatatctat aaagaagcgt tcaacagcgc atgggatcaa
     1681 tataaagata aagaagatcg gcgtgatgac gccagtcgcg aagaaaccgc gcataaagtg
     1741 gcctgggctg ctgtgaagca tgaatatgcc aaaggggatg atgataaatg gcataaaaaa
     1801 tcgtaaaacc ggtagcttag ttaaagctat tcgtgcggtg ttgccttgca agtggtccgc
     1861 ggattgcata ttgtcccgtt agtggtttca aaatgagcag taaaaatgtc cggaagacac
     1921 caaaaagttg tcgcagggaa gcatgcagtg gcggaggtgt aaggtgataa cgcgtgattt
     1981 cttgatgaat gccgattgta aaacggcatt tggtgccatt gaagaatcac tcttatggtc
     2041 agcagaacaa cgggcggctt cgctggcggc gacgctggct tgtcgacctg atgagggacc
     2101 agtgtggatc ttcggttatg gatcgttgat gtggaatccg gcactggagt ttaccgagtc
     2161 gtgcaccggt acactggttg gatggcatcg tgcgttttgc ctgcgtctga ccgccgggcg
     2221 cgggactgcg ctccagccgg gacggatgct tgcactgaaa gagggcggac gcaccacagg
     2281 cgtcgcctat cgactgccag aagagacgct ggagcaggaa ctaaccctgt tgtggaagcg
     2341 cgagatgatt accggctgtt atctgccaac ctggtgtcag cttgatcttg atgatggacg
     2401 cacagtaaac gccattgtgt ttattatgga cccgcgacat ccagaatatg aatctgacac
     2461 tcgcgctcag gtcatcgcgc cgttgattgc ggcggcgagc ggtccgctgg gaaccaacgc
     2521 acaatacctg ttttcactgg aacaggagct tatcaaactg ggaatgcagg atgatgggct
     2581 gaatgatttg ctggtatcgg taaaaaaact gctggcggag aattatccgg atggtgtgtt
     2641 gcggccggga ttcgcctgag taaacttccc tcatcgtggg gcgtcagacg ccccctcaaa
     2701 cattaaaatg tgagcacttt atcggctgac agcgtccatt gcgccagttc cacaagagta
     2761 ccgatttcca ccccatcaat cagaggaagt gtactaatcc cgcgcccgtc ggtacaggtt
     2821 ttgcacaatt tcaccggtac attctgagcg gtaaggatct ccagcatttg ctgaatgttg
     2881 tagccttccc ctggtttttg cccgcgcaac ccggctgtga ccgcatcaga catgaggaac
     2941 agacgcagat ccagattgct ctcctgctct cgtaacgcaa tggccagccg caagctgtta
     3001 aacaaggatt cgctcccgta aggtgcgcca ttggcaacga tcacaatttt ttgcattatt
     3061 tactcctgta ttcagggaat tagacactca tcttctatct tactgcttct gcagcgtctg
     3121 accaatcggt cacattttta aggattttcc tgaaagcgcg agaaaatacg acaaaagttg
     3181 ccagtaatcg ttattcttta aggctatggt ttttcatttt ttaccggaag ttaccgacgt
     3241 tttgagccgt ttcgttcctc gcattattcc gttttattta ctcttgctgg cggcaggcgg
     3301 tacagctaac gcacaatcta ccttcgagca aaaagcggca aatccctttg ataataacaa
     3361 tgatggtctg ccggatttag gcatggcacc tgaaaatcat gatggggaaa aacactttgc
     3421 tgaaattgtg aaagatttcg gcgaaaccag tatgaatgat aacgggctgg atactggcga
     3481 gcaggcaaaa gctttcgcat tgggaaaagt ccgcgacgcg cttagtcaac aggttaatca
     3541 gcacgtagag tcctggctat caccgtgggg aaatgccagt gttgacgtca aagtggataa
     3601 cgaaggacat ttcaccggca gtcgtggaag ctggtttgtg ccgttacaag ataatgatcg
     3661 ttatctcacc tggagccagc ttggtcttac tcagcaggat gatgggttgg tgagcaatgt
     3721 gggcgttggg caacgctggg cgcgcggcaa ctggctggtg ggttataaca ctttttatga
     3781 caacttgctg gacgaaaatc ttcagcgagc gggctttggt gccgaagcgt ggggcgaata
     3841 tttgcgacta tcggcaaact tttatcagcc atttgctgca tggcatgaac agacagccac
     3901 gcaggaacaa cggatggcgc gcgggtacga cctgacagcc cggatgcgca tgccgttcta
     3961 tcaacacctc aataccagtg tcagcgtaga acagtatttt ggtgatcgtg ttgatttgtt
     4021 taactctggt acgggttatc acaatcccgt cgcgttgagt ctgggattaa attacacccc
     4081 tgtgccatta gtcactgtga cggcccagca taaacagggt gaaagtggcg agaatcaaaa
     4141 taacctcggg ctgaatctta attaccgctt tggtgtaccg ctcaaaaaac aactttctgc
     4201 gggcgaggtt gccgaaagtc agtcgttacg tggtagtcgc tatgataatc cgcagcgaaa
     4261 taatctaccg actcttgagt accgacagcg aaaaacgtta acggtgtttc tggcgacacc
     4321 gccgtgggat ctaaaacctg gcgaaacagt gccgctgaaa ttacaaatcc gcagtcgtta
     4381 cggtattcgg caactgattt ggcagggcga tacgcagata ttaagtttga cgccgggcgc
     4441 acaagccaac agcgcggagg gctggacgct gatcatgcct gactggcaga acggggaagg
     4501 ggcgagcaat cactggcgat tgtcggtggt ggtggaagat aaccaggggc agcgtgtctc
     4561 ctccaatgag atcacgctaa cgcttgtcga accgttcgac gcattgtcaa acgacgaact
     4621 gcgctgggaa ccgtaatcag aaaatgcgct cctgatgcac ccataccgct gcttccacgc
     4681 gagacttgag cttcattttc ttcagcatgt gcttgacgtg cacttttact gtgctttcgg
     4741 tgatgtccag gcggcgggca atcatcttgt tcggcaaacc ctgggcaatc agcttgagaa
     4801 tatcgcgctc gcggggggtt aactggttaa catcgcgctc agtcgtggca cggttagcgc
     4861 gcaagctggc ggccagaaca ggcgttaatg cttcgcttaa taccatttcg ccagcagctg
     4921 cctgatgcaa tgctttcagc agatcttccg gttccatatc ttttaacaga tagccatccg
     4981 cgccgcgttt cagtgcggtg accacatctt cttcatggtt agagacactg aataccacaa
     5041 tgcgccctga gagggacttt tcgcgcagtt tatccagcgt ttccagaccg ttcatgccgg
     5101 gcatattgag atctaacagg atcagatcgg gatcaagaga ttccgccagt tcaataccct
     5161 gttcgccatt actcgcttcg ccaaccacgg tgatatctgg tgccatactg ataagctgtt
     5221 ttacgccagt tcgcagcatc gggtgatcgt caatcagcag gatagtagcc ggttcctgat
     5281 tactcatggg tatctccttg gacgtctgtg aaagtttttt cgggaataaa ggtgaccacc
     5341 acttcggtgc cacctgattc acgacggcgg acgcggcaat cgcctcgtaa actttgcgca
     5401 cggtcgcgca ttattatcat gccgtagtga ttgctgcgga tggcattttc aggcacgccg
     5461 cagccgttat cctggacggt cagtttgacc tgattatcgt tttgcgccac cgtcaccacg
     5521 acctcactcg cttgcgaatg tttgagggcg ttacttaatg cctcacgggc aatttgcaac
     5581 aagtggattg cctgatgcga aggcaccaga cgaggcggca attgataatc cagcttcacc
     5641 gggaagccaa atttggcgct gtactcttcg caactcgcct ccagcgccgg acgtaatcca
     5701 ggctcggtga gctgcaagcg gaatgtggtg agcaattcac gcaactgcgc ccaggatgca
     5761 ttcagttcgt tacggatctg acttaacagt tcgcggctgc tttctggcag cgcatcgccc
     5821 tgcatctgta aacaactcac ctgcatcttc atgcaagaga gagattgggc aatagaatca
     5881 tgcagttcgc gcgcaatggt ggcacgctct tccatcacga tcaactgttg ctgacgttcc
     5941 tgatgccgat ccagcgccag cgtggcggtg agttgttcaa ccagggtatc caccagttgt
     6001 tgttgatcat ggctaagatg acgcccctgc ggcagggtcg ccagcaaaat accgtactgc
     6061 gtatgagagt cagccagccg ccacttcagg gtcgtgccgc gatcaccaac gggtaatacg
     6121 ccgcgcgggc agagctggca gcctttatca tcacaagtca tatctggctg gcaggtaaac
     6181 tcctgatgat tctcttcatc atcagtgtca tacacccgca gttcgatatc acgtagcagg
     6241 gttaaattct gtaagccgtt gagtacaggt gacaggcgtt cacacagcgg ggcgcgggaa
     6301 tgcaaacggc ggttagcctg ccataaaaaa gagaggatct gatttttatg ctccagcccg
     6361 gcggttttct cctgaacccg ctgctcaagt acggcataac tttcggccag ttctgcagac
     6421 atattgttca acgcagttcc aagcatcgcc atttcgttgc gcccgctgat gtttgcgcgt
     6481 tgggtaaaat cgcgatggct gacggcactc gccattgcca gcagttgccg ccacggttgt
     6541 agcagtcgcg cccgcaacca gataatagtg aacaccagta aaagtgccat aaataccgcc
     6601 attacccgat ggaccagtac cactgtctca atacgcattt ccgtggtgcg gtcaaaacca
     6661 gataccagct gatcaagccc ggcaacaaac tggctgacat ccgctgatac cgtttctcgg
     6721 ttttgcgccc gcatcagcgc agggatcagt tcattacgcc agtaatcttg taaaccctgt
     6781 aattgcgcca gttgtccgtc tcgttctgct gctcgagtca actcggcgct aaatgccgtt
     6841 tgttccatct ctttaattaa gggcttgtct ttctcgctta atggcactgc tgccaacaga
     6901 cggtaacttt gcatgcgcag cgatcccgct ttgttgatcg catgggcgct gccctgaacg
     6961 ccttgcacca gccagccaga aaccgccatc cctgccagtc caatagcagt agaaagcaac
     7021 acaataagcg caacctgatt aaccagggtg agcggagaga gacaacgttt aagcatgtaa
     7081 acctcttcct tcaggcttta aatgagcaat aaccttaatg aatgtgacga tacattctgg
     7141 aatggcagta ttctcggcta ttggctgaag tatacccata cccggaaaga gttactcctt
     7201 atttgccgtg tggttagtcg ctttacatcg gtaagggtag ggattctaca gcaccgtgaa
     7261 aaatctcata atttttatga aatcactgta ctcactatgg gtaatgataa atatcaatga
     7321 tagataaagt tatcttatcg tttgatttac atcaaattgc ctttagctac agacactaag
     7381 gtggcagaca tcgaaacgag tatcagaggt gtctatgagt cactcatccg cccccgaaag
     7441 ggctactgga gctgtcatta cagattggcg accggaagat cctgcgttct ggcaacaacg
     7501 cggtcaacgt attgccagcc gcaacctgtg gatttccgtt ccctgtctgc tgctggcgtt
     7561 ttgcgtatgg atgttgttca gcgctgttgc ggtgaactta ccgaaagtcg gttttaattt
     7621 tacgaccgat cagctattta tgttgactgc gctgccttcg gtttctggcg cgttattacg
     7681 tgttccatac tcctttatgg ttcctatctt cggtggtcgt cgctggacgg cgttcagcac
     7741 cggtattctg attattcctt gtgtctggct gggttttgcc gtgcaggata cctccacgcc
     7801 ttatagcgtc ttcatcatca tctctctgct gtgcggcttt gctggcgcga acttcgcttc
     7861 cagtatggca aacatcagct tcttctttcc gaaacagaag cagggtggcg cgctgggcct
     7921 gaatggtggt ctgggcaaca tgggcgttag cgttatgcag ttggttgctc cgctggtggt
     7981 atcactgtcg attttcgcag tatttggtag ccagggcgtc aaacagccgg atgggactga
     8041 gctgtatctg gcgaatgcgt cctggatatg ggtgccgttc cttgccatct tcaccattgc
     8101 ggcgtggttt ggcatgaacg atcttgctac ctcgaaagcc tccatcaagg agcagttgcc
     8161 ggtactcaaa cggggtcatc tgtggattat gagcctgctg tatctggcaa ccttcggctc
     8221 ctttatcggc ttctccgcgg gctttgcgat gctgtcaaaa acgcagttcc cggatgttca
     8281 gattctgcaa tacgctttct tcgggccgtt tattggtgcg ctggcgcgtt ctgcaggtgg
     8341 tgcattatct gaccgtctgg gcggaactcg tgtcacgctg gtgaacttta ttctgatggc
     8401 gattttcagc ggcctgctgt tcctgacctt accgactgac gggcagggcg gaagcttcat
     8461 ggcgttcttc gcggtcttcc tggcgctgtt cctgacagct gggctgggta gtggttccac
     8521 tttccagatg atttcagtga tcttccgtaa actgacaatg gatcgcgtga aagcagaagg
     8581 gggttctgac gaacgtgcga tgcgtgaagc ggcaaccgac acggcggcgg cgctgggttt
     8641 catctctgcg attggcgcga ttggtggctt ctttatcccg aaagcgtttg gtagctcgct
     8701 ggcattaacg ggttcgccag tcggcgcaat gaaggtattt ttgattttct atatcgcctg
     8761 cgtagtgatt acctgggcgg tatatggtcg gcattctaaa aaataaatct taatatttga
     8821 ttatcctttg cgcggcatga tgtcgcgctt tttttatgcg t
//
LOCUS       DAZFKF010000160         8717 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFKF010000160 DAZFKF010000000
VERSION     DAZFKF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.162"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(6..761)
                     /gene="mlaA"
                     /locus_tag="ACHKA1_004822"
     CDS             complement(6..761)
                     /gene="mlaA"
                     /locus_tag="ACHKA1_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO5682370.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            1055..1987
                     /gene="yfdC"
                     /locus_tag="ACHKA1_004823"
     CDS             1055..1987
                     /gene="yfdC"
                     /locus_tag="ACHKA1_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO5682371.1"
                     /translation="MDNDKIDQHSDEIEVESEEKESGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2063..2137
                     /locus_tag="ACHKA1_004824"
     tRNA            2063..2137
                     /locus_tag="ACHKA1_004824"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2096..2098,aa:Arg,seq:cct)
     gene            2299..3456
                     /gene="intS"
                     /locus_tag="ACHKA1_004825"
     CDS             2299..3456
                     /gene="intS"
                     /locus_tag="ACHKA1_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFO5682372.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IISLIATKVLRYTALRTKELRSMQWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWIDEKVE"
     gene            3829..4782
                     /gene="ompT"
                     /locus_tag="ACHKA1_004826"
     CDS             3829..4782
                     /gene="ompT"
                     /locus_tag="ACHKA1_004826"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO5682373.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            4969..6453
                     /locus_tag="ACHKA1_004827"
     CDS             4969..6453
                     /locus_tag="ACHKA1_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5682374.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKPENIYCPGNSEAEIQ"
     gene            complement(6637..6849)
                     /locus_tag="ACHKA1_004828"
                     /pseudo
     CDS             complement(6637..6849)
                     /locus_tag="ACHKA1_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            6999..7667
                     /locus_tag="ACHKA1_004829"
     CDS             6999..7667
                     /locus_tag="ACHKA1_004829"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5682375.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(7612..7749)
                     /locus_tag="ACHKA1_004830"
     CDS             complement(7612..7749)
                     /locus_tag="ACHKA1_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFO5682376.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(7722..8306)
                     /locus_tag="ACHKA1_004831"
     CDS             complement(7722..8306)
                     /locus_tag="ACHKA1_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5682377.1"
                     /translation="MAFRMSEQPRTIKIYNLLSGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(8306..>8717)
                     /locus_tag="ACHKA1_004832"
     CDS             complement(8306..>8717)
                     /locus_tag="ACHKA1_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682378.1"
                     /translation="SSTDLGTKTTSSFDYGTKSTNNIGAHTHSVSGTAASAGNHTHSV
                     TGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHS
                     VAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT         2560 a         2015 c         1910 g         2232 t
ORIGIN      
        1 gtttcttatt cagaatcgat atcttttaaa tcatcctgaa tcgcttgcgc gttcggattt
       61 tcctgcggtt tgagttcgcc gccattagcg atgaaatcat gacgctggaa gtacgcttcg
      121 cgcaccataa tataaggatc ggacgactga cgcagcagac cgtcggaatc caacaactgc
      181 gcacgagttt ctatcccttc aagcgtccat ttacccacag acatcggcca ggtcagccag
      241 gatagaaccg ggtaaagacc atccgccata tcaccaccgt catcacgcag cgtgaagcta
      301 ccgtagaacg gtaactggac gtaaggccca taacccacgc cataatgacc aagcgtacta
      361 ccgaagcggt gaggttcagt ccgttgcagt ttcgggttcg ccatccctgc aacatcaata
      421 aaaccaccca tccccaaaat ggtgttcagg aaaaagcggg taaagtggac catcccctga
      481 taagggtcgc cctgcaagaa gtagttcacc atcaccgcgg gttcttcaag gttgccggta
      541 aagttgctca aaccgttacg cgccggttgc ggaacataat cacgccaggc gacagcgacc
      601 ggtcgaacaa tatacgggtc tagtacgttg aagttgaagt tgtacatggt gcggttgaac
      661 ccttctaacg ggtcagaacg cccttgctga tctgtaccgg aactcgcaca ccccaccaga
      721 agcgtagttc ccagagcaag cgccgacagg cgaagcttca taaatgtctc cctgtttttt
      781 tatggcttat gcagtttgcc atccatgacg gaacgatacc gtatccgcct gtttaggtgt
      841 gggcgattgt aacagcacgt caactgatgt ccagacgccc tgatttgctg atttgatcat
      901 agcctggtaa tcgccgccct gtaggctact tgattctata gaaacagaaa aaggcaaacg
      961 cggccttttc tacattttca gagtaactcc cgccagttgc gagcgaaaaa gccgctacgc
     1021 tttagctata cgtgctaatt caagagaaga gaccatggac aacgacaaaa ttgatcaaca
     1081 cagcgacgaa attgaagttg agagcgaaga aaaagagagt ggcaaaaaaa tagaaataga
     1141 tgaagatcga ctcccctccc gggcgatggc aattcatgag catatccgcc aggatggtga
     1201 aaaagagctg gaacgcgacg caatggcact actgtggtca gccattgcgg cgggtctgtc
     1261 gatgggcgct tcattactgg caaaagggat atttcatgtc gaactggaag gtgtgccggg
     1321 cagcttcttg ctggagaatc taggttatac ctttggtttt attatcgtca ttatggcccg
     1381 ccagcaatta tttaccgaaa acaccgtgac tgctgtacta cccgtcatgc aaaaaccgac
     1441 aatgagcaac gttggcttac ttatgcggtt atggggcgtc gtgctgctgg gtaatattct
     1501 cgggacaggt attgcggcgt gggcatttga atatatgcca atcttcaatg aagaaactcg
     1561 cgatgcattt gtcaaaatcg gcatggatgt gatgaagaac acccccagcg aaatgtttgc
     1621 caacgcgatc atttccggct ggctgatcgc cactatggta tggatgtttc ctgcagcggg
     1681 tgcggcaaag attgtggtga ttatattgat gacctggctt attgccctgg gtgacaccac
     1741 ccacatcgtt gtcggttctg ttgaaatcct ctatctggtg tttaacggca cgctgcactg
     1801 gagcgatttc atctggccct tcgcactacc tactttagcg gggaacatct gcggcggcac
     1861 ctttatcttc gcgttaatga gtcatgcaca gatccgtaac gacatgagca acaagcgcaa
     1921 agcagaagca cgccaaaaag cagaacgtgc ggaaaacatt aagaaaaatg ataaaaaccc
     1981 ggcataaatg gcgagggttt aagcaatcgc acggcagcgt acttaccccg cagtccatta
     2041 gcgggtatac tcattccgca ttgtcctctt agttaaatgg atataacgag cccctcctaa
     2101 gggctaattg caggttcgat tcctgcaggg gacaccattt atcagttcgc tcccatccgt
     2161 accagtccgc aaaatcccct gaatatcaag ccttccgtag attcacagtt cgtaatggtt
     2221 cgcgtcagat cgttgacagc cgcactccat gacgggtaaa aagtggataa aataatttta
     2281 cccaccggat ttttacccat gctcaccgtt aagcagattg aagcagcaaa gccgaaagaa
     2341 aaaccatacc gcctactcga tggtaatggc ctgtaccttt atgtccctgt gtcagggaaa
     2401 aaggtatggc agcttcgcta caagattgac ggtaaggaga aaatcctgac cgtaggaaaa
     2461 tatccgctaa tgactttgca ggaggcaaga gataaagcat ggaccgcgag gaaagacatc
     2521 tcggttggca tcgatccggt aaaagcgaaa aaggcttcgt ctaacaacaa ttcctttagt
     2581 gcgatttaca aggaatggta cgagcacaag aagcaagtct ggtcagtagg ctatgcaact
     2641 gaacttgcaa aaatgtttga tgacgacatt ttacctatca tcggcggcct tgaaattcag
     2701 gatattgagc cgatgcaact gctggaagta atccgcagat ttgaagatcg cggtgcaatg
     2761 gagcgagcca acaaagcccg cagaagatgc ggcgaggttt tccgttacgc tattgtcact
     2821 ggtagggcta aatataaccc ggcacctgac cttgctgacg ccatgaaggg ataccgcaag
     2881 aagaacttcc cgtttctccc agcagaccag atccctgcat tcaacaaagc actggcaaca
     2941 ttttcaggaa gcatcatatc gctcattgcg accaaagttt tacgctacac agccctaaga
     3001 acgaaagagc ttcgctccat gcaatggaag aacgtcgatt ttgaaaacag gattatcact
     3061 atcgacgcca gtgtgatgaa gggccgcaag attcatgttg ttcctatgtc agaccaggtg
     3121 gttgaacttc tcactacgct aagctccatc actaaaccag tatcagagtt tgtttttgcc
     3181 gggcgcaacg ataagaagaa gccaatctgc gagaacgcgg tattgcttgt gatcaaacaa
     3241 atcggctatg agggtctgga aagcggtcac ggattcaggc atgaattcag cacaattatg
     3301 aacgagcacg aatggcctgc tgacgctatt gaagtgcaac tggcacatgc caacggcgga
     3361 tctgtgcgtg ggatttacaa ccatgctcag tatctcgata aacgcagaga aatgatgcaa
     3421 tggtgggcgg actggattga tgaaaaggtg gagtgatcca ccttaaccac tatcgaagag
     3481 cacaaagcct tgcaatccag tacaaagcta tgtgtgtctc agttttgtcc ctcttttttg
     3541 tactaaaaaa catagcaatt gaggataaac ctcatgctat tttcgcttat atgcctttaa
     3601 aggcatggcg cttaaataga taaaaacacc acaaaagcat aaaaaaccac acagtaaaac
     3661 cgcaacataa aacaataaca gataattaaa ccaaaaacag atagcgcatt gtgataatca
     3721 ttcaatgcta aacaaaatat aaacagtgga gcaatatgtg attgactcat taagttagat
     3781 ataaaaaata catattcaat cattaaaacg attgaatgga gaacttttat gcgggcgaaa
     3841 cttctgggaa tagttctgac aacccctatt gcgatcagct cttttgcttc taccgagact
     3901 atatcgttta ctcctgacaa cataaatgcg gacattagtc ttggaactct gagcggaaaa
     3961 acaaaagagc gcgtttatct agctgaagaa ggaggccgaa aagtcagtca actcgactgg
     4021 aaattcaata acgctgcaat tattaaaggt gcaattaatt gggatttgat gccccagata
     4081 tctattgggg ctgcgggctg gactactctc ggcagccgag gtggcaatat ggtcgatcag
     4141 gactggatgg attccagtaa ccccggaacc tggacagatg aaagtaggca ccctgataca
     4201 caactcaatt atgccaacga atttgatctg aatatcaaag gctggctcct caacgaaccc
     4261 aattaccgcc tgggactcat ggccggatat caggaaagcc gttatagctt tacagccaga
     4321 ggtggttcct atatctacag ttctgaggag ggattcagag atgatatcgg ctccttcccg
     4381 aatggagaaa gagcaatcgg ctacaaacaa cgttttaaaa tgccctacat tggcttgact
     4441 ggaagttatc gttatgaaga ttttgagcta ggtggcacat ttaaatacag cggctgggtg
     4501 gaagcatctg ataacgatga gcactatgac ccaggaaaaa gaatcactta tcgcagtaaa
     4561 gtcaaagacc aaaattacta ttctgttgca gtcaatgcag gttattacgt aacacctaac
     4621 gcaaaggttt atgttgaagg cgcatggaat cgggttacga ataaaaaagg taatacttca
     4681 ctttatgatc acaatgataa cacttcagac tacagcaaaa atggtgcagg catagaaaac
     4741 tataacttca tcactactgc tggtcttaag tacacctttt aacgaagtta accagatttt
     4801 ctccccggtg gtattgtata accccgggga atttgtttac agttacaagt aggcaatatc
     4861 cactaatttt gtaagcattt aacctatgtc ataacgtgta atctcttacc gcaacacaag
     4921 acctcaatat attcaaatct tatctttatt ccgatattca gagacacaat gcgaaaaata
     4981 attactcatt tcaaagttgt tttaacgtta cttctaccag taaccgtatc tgcccagcag
     5041 atacagtggc aatcctgtat ggccagtcaa ttcaaccact ggtttggtga ggaaaaaccg
     5101 tctcctgact tactatgtgg ttatttgtct gttccattaa aatatacaga cacaggcgga
     5161 gatgcttctt atgaaaaaaa atcacaagtc aaactagcgt tgacaaaatt gccggcaaaa
     5221 agcaagcata aaggaagtat cctgataata agtggtggtc ccgggttacc aggcataaat
     5281 ccttatatta actttgactg gccagtcaca aatcttcgtg agtcatggga tattattgga
     5341 tttgatcctc gaggcgtcgg acagtccact ccgacaataa actgccggca atcagataca
     5401 gagactcagg aaaacataac cgaaaagcaa caagtattaa ataaaattaa tgcctgtatc
     5461 cataataccg gagccgaagt cattcgccat atcggctcta acgaggctgt atacgatatt
     5521 gatcgtatta ggcaagcctt gggggataaa caactgacag ccgtggcgta ttcgtatgga
     5581 actcaaattg cagccttata tgcagaacgt tttccctaca acgtaagatc tatcgttctt
     5641 gatggagtcg tcgatatcga tgacctggag gacaacttca catggcaact caaacaggca
     5701 cagagttatc aggaaacgtt tgatcgcttt gcatcctggt gtgcgcgtac aaaaagttgc
     5761 ccgctttctt cagacagaga taaggcaata actcagttcc atgagctatt atcaaaatta
     5821 catcacaaac ctttattaga cagtaaggga gaaaatatat cttcagatga actcatatca
     5881 ttaacaacag accttctgct atggcgttca tcatggccaa cccttgcaac tgccatacgc
     5941 cagttctctc aggggattgt cagtaatgaa attgaaactg cgctcagtgc tccgatagcc
     6001 tcagaagagt caagcgatgc ttcgggggta atcctctgtg tagatcaggg ggatgagcaa
     6061 ttaacaccag aagagcgaaa atcccgaaaa gacgctcttg cgaatgcctt cccggctatt
     6121 aactttgaca atggacgttc cgattcacct gatttttgtg aattatggcc aatacatagc
     6181 gacctgaaca aaactcgcct gaaaaatact gttctgccct ctggtttact gtttgtagca
     6241 cacaaatacg acccaacaac gccctggatt aatgcccgta agatggcaga gaaattttcc
     6301 agcccgttac taacaataaa tggtgatggg catacattag ctctcaccgg agttaattta
     6361 tgtgtagata aagcagttgt acatcacctg atcactccac aaaaaccaga aaatatatac
     6421 tgcccaggaa attctgaagc agaaatacaa taatttcaaa atattccgct atttgctccc
     6481 aatgcaaaac atatattgca ttgggagcat attcatattt ttgttattct tgtatgaaat
     6541 cgtctcatgt acacggtcct tttttcgccg aaaactgata ttactttaat caaaagttga
     6601 cgtgccagat tcgaagtaaa tttataaaat atcaaatcaa tatacatttt gcaccttcga
     6661 gatagtaatg gctgttactg taacatgagt tacaaacagc ttcataccca gagctactga
     6721 tgtatcaact cgattcaaca atacccgata tttattccat gcctccagca acgatctttc
     6781 ttcctccgtt gcgatttcca gatctacagc atcctgcagt ggcgcaatat actcactgaa
     6841 ttcctggatg tagaactgtg tggtgacggt cttccagcca ttcggctcct gctgtatcga
     6901 agcataccat gctatttcaa tatcgctatg ctgcggcagc atttaacccc ttgtaattca
     6961 tcgccataat tgatttaatt cacaaataaa actataacat ggtgaaatta atgaaaaaaa
     7021 acacagatga tggggctaaa atttacacac cacttaccct aaagctttat gactggtggg
     7081 ttttgggagt atcaaatcgg cttgcatggg gatgtcctac aaaggaacac cttcttccac
     7141 actttctgga acatttaggt aacaaccatc tggatattgg tgttggaact gggttttacc
     7201 ttactcacgt acctgagagt agtctgatat ctttaatgga tttgaacgaa gctagcctga
     7261 acgcggcatc tacaagggct ggggaatcaa aaattaaaca taaaattaac catgatgttt
     7321 ttgaacctta tcccgcggcg ttacatggtc aatttgattc catttccatg ttttaccttc
     7381 ttcactgcct gcctggaaat atatctacaa aaagctgtgt aatacgcaat gcggcgcagg
     7441 ccttaactga cgatggaact ctatacggag ccacaattct tggcgatgga gttgtgcaca
     7501 atagcttcgg tcaaaaactg atgcgcattt acaatcagaa aggcatcttt tcaaacacaa
     7561 aagattccga agaaggctta acacatatac tctcagagca tttcgagaat gttaaaacca
     7621 aggttcaagg tactgtagta atgttttccg cttcagggaa aaaatagcat ccaaccgcag
     7681 cacgttcttg cttaagacgt gctgcggcat aatcccaatg attactccct gacagggttc
     7741 gtaggccact caatatcagg tgcagttgat gtatcaacac ggttcagcaa cacccgatac
     7801 tttttccagg cttccagcaa caaggtttct ttctccgttg cgatctccag atccacagca
     7861 tcctgaagcg gcgctatatg ctcactggct acctgcatca ggctgttttt tgtttcttcc
     7921 gcctcccgga tccggaacag tttttctgct tccgtatcct tcacccaggc tgtgccgttc
     7981 cacttctgaa actccccttc cggggataac caggtgacat tttccggtaa tgagccgagt
     8041 tcagaaataa ataacgcgtc gccggaagcc acgtcataaa ccgttttacc ccgatgatct
     8101 tcaacgagat gccacgatga ctcatcactg ttgaaaacag ccacgaaacc agccggaata
     8161 tctggcggtg caatatcggt actgtttgct ggcagacctg tatgaggcgg aatatatgca
     8221 tcaccttcac caataaattc attagttccg gacagcagat tataaatttt tatggtccgt
     8281 ggttgttcac tcattctgaa tgccattatg caagcctcac aatatagtta aatgcgatgt
     8341 ttttgacggt gttttccgcg ttaccagcag cgttaacggt gatggtgtgt ccatgtgagc
     8401 caatcgcaac cgagtgcgta tgagcaccaa taccgacagt atgtgcgtga gcacctgcag
     8461 atgcagctgt gccgctgaca ctatgagtgt gcgctcctgc agcacttgta ttcacactgg
     8521 ccgcagacac taccttatgt actgacccat tttgtgacca ctggctgact gctgatgcgc
     8581 ctgtgacact atgagtatgg tttccggcac ttgcggctgt accgctcaca ctgtgcgtat
     8641 gcgccccgat gttattcgtg gatttagtgc cgtaatcaaa cgacgatgtg gttttcgtcc
     8701 ccaaatccgt actggat
//
LOCUS       DAZFKF010000161         8676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFKF010000161 DAZFKF010000000
VERSION     DAZFKF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.163"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            241..2664
                     /gene="pgaA"
                     /locus_tag="ACHKA1_004833"
     CDS             241..2664
                     /gene="pgaA"
                     /locus_tag="ACHKA1_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO5682379.1"
                     /translation="MYSSSRKRCPQTKWALKILTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALLWFAQKSALSNNQIADWLQIALWAGQDKQVITVYNRYRYQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWAQYWVASAYLKEHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQTRGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLNDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERFFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSIE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2673..4691
                     /gene="pgaB"
                     /locus_tag="ACHKA1_004834"
     CDS             2673..4691
                     /gene="pgaB"
                     /locus_tag="ACHKA1_004834"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO5682380.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSKTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFRWPAVWAPVGSWVDTPADEQVKFGDEMVD
                     REYFATWQQVREVARSPLIEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAKVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     AGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGLLFHDDALLSDYEDASAPAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVTQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVAEKSADQWLIQLINQIKSIPQAKDKSILELQAKNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            4684..6009
                     /gene="pgaC"
                     /locus_tag="ACHKA1_004835"
     CDS             4684..6009
                     /gene="pgaC"
                     /locus_tag="ACHKA1_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO5682381.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6011..6424
                     /gene="pgaD"
                     /locus_tag="ACHKA1_004836"
     CDS             6011..6424
                     /gene="pgaD"
                     /locus_tag="ACHKA1_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO5682382.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6474..7262)
                     /gene="phoH"
                     /locus_tag="ACHKA1_004837"
     CDS             complement(6474..7262)
                     /gene="phoH"
                     /locus_tag="ACHKA1_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO5682383.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7894..8301)
                     /locus_tag="ACHKA1_004838"
                     /pseudo
     CDS             complement(7894..8301)
                     /locus_tag="ACHKA1_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
     gene            complement(8429..>8676)
                     /locus_tag="ACHKA1_004839"
     CDS             complement(8429..>8676)
                     /locus_tag="ACHKA1_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682384.1"
                     /translation="IFYELDGEWYHEICRNAIKRGGQRGEHLRANIDRASVHKFRQFR
                     YIRFLNKRARKRLNTKLFKVQPYPKPQTVKTGLKESE"
BASE COUNT         2317 a         2033 c         2034 g         2292 t
ORIGIN      
        1 gaaaaaggca ttgggattta tgccgtattt ctgaagatcc tcatcattgg aatggatttt
       61 cgggcgagaa aaggatttta tatggacact ctgctcatca tttcttcttc tcatcatcaa
      121 caattcacgt ctctcttccg cgtttaataa cggattatga ggtgcaaaaa atatctttct
      181 tttcagttgc ctgtcattag atacagagag agattttggc aatacatgga gtaatacagg
      241 atgtattcaa gtagcagaaa aaggtgcccg caaaccaaat gggctttgaa aattcttact
      301 gccgcatttt tagcagcgag tcccgcggcg aagagtgccg ttaataacgc ctatgatgca
      361 ttgattattg aagctcgcaa gggaaatact cagccagctt tgttatggtt tgcacaaaaa
      421 tcagcactca gtaataacca aattgctgac tggttacaga ttgccttatg ggccgggcaa
      481 gataaacagg ttattaccgt ttacaaccgc taccgttatc agcaattacc agcgcgtggt
      541 tatgcagctg tcgccgtcgc ttatcgtaac ctgcaacaat ggcaaaactc gcttacactg
      601 tggcaaaagg cgctctctct ggagccgcaa aataaggatt atcaacgggg acaaatttta
      661 accctggcag atgcgggtca ctatgatact gcgctggtta aacttaagca gcttaactct
      721 ggagcaccgg acaaagccaa tttactcgca gaagcctata tctataaact ggcagggcgt
      781 catcaggatg aattacgggc gatgacagag tcattacctg aaaatgcctc tacgcaacaa
      841 tatcccacag aatacgtgca ggcattacgt aataatcaac ttgctgccgc gattgacgat
      901 gccaatttaa cgccagatat tcgcgctgat attcatgccg aactggtcag actgtcgttt
      961 atgcctacgc gcagtgaaag tgaacgttat gccattgccg atcgcgccct cgcccaatac
     1021 gctgcattag aaattctgtg gcacgataac ccagaccgca ctgcccagta ccagcgtatt
     1081 caggttgatc atcttggcgc gttattaact cgcgatcgtt ataaagacgt tatttctcac
     1141 tatcagcgtt taaaaaagac ggggcaaatt attccgccct gggcgcaata ttgggttgcg
     1201 tcggcttatc tcaaagaaca tcagccaaaa aaagcacagt caataatgac agagctcttt
     1261 tatcacaagg agaccattgc cccggattta tccgatgaag aacttgcgga tctcttttac
     1321 agccacctgg agagtgaaaa ttatcccggc gcgctaactg tcacccaaca taccattaat
     1381 acttcgccgc ctttccttcg gttaatgggc acgcctacga gcatcccgaa tgatacctgg
     1441 ttacaggggc attcgtttct ctcaaccgta gcaaaatata gtaatgatct tcctcaggct
     1501 gaaatgacag ccagagagct tgcttataac gcaccaggaa atcagggact gcgcattgat
     1561 tacgcgagtg tgttacaaac ccgcggttgg cctcgtgcag cagaaaatga attaaaaaaa
     1621 gcagaagtga tcgagccacg taatattaat ctggaggttg aacaagcctg gacagcatta
     1681 acgttacaag aatggcagca ggcagctgtc ttaacgcacg atgttgtcga acgtgaaccg
     1741 caagatcccg gcgttgtacg tttaaaacgt gcggttgatg tacataatct tgcagagctt
     1801 cgtatcgctg gctcaacagg aattgatgcc gaaggcccgg atagtggtaa acatgatgtc
     1861 gacttaacca ccatcgttta ttcaccaccg ctaaatgata actggcgcgg ttttgctgga
     1921 ttcggttatg ccgatggaca atttagcgaa ggaaaaggga ttgttcgcga ctggcttgcg
     1981 ggtgttgagt ggcggtcacg taatatctgg ctcgaggcgg agtacgctga acgctttttc
     2041 aatcatgagc ataaacccgg cgcgcgcctg tctggctggt atgattttaa tgataattgg
     2101 cgtattggtt cgcaactgga acgcctctct caccgcgttc cattacgggc aatgaaaaat
     2161 ggtgttacag gcaacagtgc tcaggcttat gttcgctggt atcaaaatga gcggcgtaag
     2221 tacggtgtct cctgggcttt cactgatttt tccgacagta accagcgtca tgaagtctca
     2281 attgagggtc aggaacgcat ctggtcttca ccatatttga ttgtcgattt cctacccagt
     2341 ctgtattacg aacaaaatac agaacacgat accccatact acaaccctat aaaaacgttc
     2401 gatattgttc cggcatttga ggcaagtcat ttgttatggc gaagctatga aaatagctgg
     2461 gagcaaatct tcagcgcagg tgttggtgcc tcctggcaaa aacattatgg cacggatgtc
     2521 gtcacccaac tcggctacgg gcaacgcatt agctggaatg acgtgattga tgctggcgca
     2581 acgctacgct gggaaaaacg accttatgac ggtgacagag aacacaactt atacgttgaa
     2641 ttcgatatga cattcagatt ttaaggataa atatgttacg taatggaaat aaatatctcc
     2701 tgatgctggt gagtataatt atgctcactg cgtgcattag ccagtcaaaa acatcattta
     2761 taccgccaca ggatcgcgag tctttactcg ccgagcaacc gtggccgcat aatggttttg
     2821 tggcgatctc atggcataac gttgaagacg aagctgccga ccagcgtttt atgtcagtgc
     2881 ggacatcagc actgcgtgaa caatttgcct ggctgcgcga gaacggttat caaccggtca
     2941 gtattgctca aattcgtgaa gcacatcgag gaggaaaacc gctaccggaa aaagcagtag
     3001 tgctgacttt tgatgacggc taccagagtt tttatacccg cgtcttccca attcttcagg
     3061 ccttccggtg gccagctgta tgggcccccg tcggcagttg ggtcgatacg ccagcggatg
     3121 aacaagtaaa atttggcgat gagatggtcg atcgagaata ttttgccacg tggcaacaag
     3181 tgcgagaagt tgcgcgttcc ccgctcattg aggtcgcttc tcatacatgg aattctcact
     3241 acggtattca ggctaatgcc accggcagct tattgcctgt atatgtaaat cgtgcatatt
     3301 ttactgacca cgcacggtat gaaaccgcag cagaataccg ggaaagaatt cgtctggatg
     3361 ctgtaaaaat gacggaatac ctgcgtacaa aggctaaggt aaatccacac gtttttgttt
     3421 ggccttatgg cgaagcgaat ggcatagcga tagaggaatt aaaaaaactc ggttatgaca
     3481 tgttcttcac ccttgaatca ggtttggcaa atgcgtcgca attggattcc attccgcgag
     3541 tattaatcgc caataatccc tcattaaaag agtttgccca gaaaattatt accgtacagg
     3601 aaaaatcacc acaacggata atgcatatcg atcttgatta cgtttatgac gaaaaccgcc
     3661 agcaaatgga tcgcaatatt gatgtgctaa ttcagcgggt gaaagatatg caaatatcaa
     3721 ccgtgtattt gcaggcattt gctgatcccg atggtgatgg gctggtcaaa gaggtctggt
     3781 ttccaaatcg tttgctacca atgaaagcag atatttttag tcgggttgcc tggcaattac
     3841 gtacccgcgc aggtgtaaac atctatgcgt ggatgccggt attaagctgg gatttagatc
     3901 ccacattaac gcgagtaaaa tacttaccaa ctggggagaa aaaagcacaa attcatcctg
     3961 aacaatatcg ccgtctctcg ccttttgatg acagggtcag agcacaagtt ggcatgttat
     4021 atgaagatct tgccggacat gctgcttttg atggcctatt gttccacgat gatgctttgc
     4081 tttcagatta tgaagatgcc agtgcaccgg ctatcgcggc ttatcagcaa gcaggcttta
     4141 gcgggagtct gagcgaaatt cgacaaaacc cggagcaatt taaacagtgg acccgcttta
     4201 aaagtcgtgc gttaactgac ttcactttag aacttagtgc gcgcgtaaaa gccattcgtg
     4261 gtccacatgt taaaactgca cgaaatattt tcgcacttcc ggtaacacaa cctgaaagtg
     4321 aagcctggtt tgcacagaat tatgctgatt tcctaaaaag ctatgactgg actgctatta
     4381 tggctatgcc ttatatggaa ggtgtcgcag aaaaatcggc tgatcaatgg ttaatacaac
     4441 tgatcaatca gattaaaagc atccctcagg ctaaagacaa atctatttta gaattacagg
     4501 caaaaaactg gcagaaaaat ggtcagcatc aggctatttc ttcgcaacaa ctcgctcact
     4561 ggatgagcct attacaactg aatggagtga aaaactatgg ttattatccc gacaattttc
     4621 tgcataacca acctgaaata gacgttattc gtcctgagtt ttcaacagcc tggtatccga
     4681 aaaatgatta accgcatcgt atcgtttttt atattatgtc tggtgttatg catcccccta
     4741 tgcgtagcgt actttcactc tggtgaactg atgatgaggt tcgttttctt ctggccgttt
     4801 tttatgtcca tcatgtggat tgttggcggc gtctatttct gggtctatcg tgaacgccac
     4861 tggccgtggg gggaaaacgc accagctccc cagttgaaag ataatccgtc tatctccatt
     4921 atcattccct gttttaatga ggagaaaaac gttgaggaaa ccatacacgc cgctttagca
     4981 cagcgttatg agaacattga agttattgcc gtaaatgacg gttcaacaga taaaacccgt
     5041 gccatcctgg atcgcatggc tgcacaaatt ccccatttgc gggtcattca tctggcgcaa
     5101 aaccagggga aagccattgc gcttaaaacc ggagctgccg cggcgaaaag tgaatatctg
     5161 gtgtgcattg atggcgacgc gttattagac cgcgatgcgg cggcatatat tgtggaaccg
     5221 atgttgtaca acccgcgtgt gggtgccgta accggtaatc ctcgtattcg cacacgttct
     5281 accctggtgg gtaaaattca ggttggcgag tattcctcaa ttattggttt gatcaagcga
     5341 acccagcgta tctatggaaa cgtatttacc gtttccggtg ttattgccgc atttcgtcgc
     5401 agcgccctgg cagaagtggg ttactggagt gacgatatga tcaccgaaga tattgatatt
     5461 agctggaagc tgcagttgaa tcagtggacg attttttacg agccacgggc gctgtgctgg
     5521 atattaatgc ctgaaacgtt aaaagggctg tggaaacagc gcctgcgctg ggctcagggc
     5581 ggtgcagaag tattcctcaa aaatatgaca aggttgtggc gcaaagaaaa ctttcgaatg
     5641 tggccactgt tttttgaata ctgcctgacg acaatatggg cctttacctg cctggtcggt
     5701 ttcattattt acgcagtcca acttgccggt gtaccgttaa atattgaatt gacacatatc
     5761 gctgcgacac atactgccgg aatattattg tgtacgttat gtttactgca atttattgtc
     5821 agcctgatga ttgagaatcg ctatgagcat aatctgactt catcgctttt ctggattatt
     5881 tggttcccgg ttattttctg gatgctgagc ctggcaacga cattggtatc atttacacga
     5941 gtcatgttga tgcctaaaaa gcaacgcgcc cgttgggtaa gtcccgatcg cgggattctg
     6001 agaggttaat atgaacaatt taattattac gacccgacaa tcgccagtac gtttactggt
     6061 tgattatgtt gccacaacca tcttgtggac attatttgct ttgttcatat tcttattcgc
     6121 catggatctg ctgacgggtt attactggca aagcgaggcc agaagccgac ttcagttcta
     6181 ttttttgctg gcagtggcga atgccgtcgt gttaattgtc tgggcgctgt acaataagct
     6241 gcgttttcaa aaacagcagc atcatgcagc ctaccaatat acgccgcaag aatatgcaga
     6301 gagcttagca atacctgatg agctctatca gcaactacaa aaaagccaca ggatgagcgt
     6361 acacttcacc agccaggggc aaataaaaat ggtggtttca gaaaaagcgc tagtccgggc
     6421 ataaacaccc aaaacaaagc ccggttcgcc cgggctctgc accgataata cacttaactg
     6481 tatgcatgca gcgtacgttg gcaaagtgcc gaacgtacgc aatcctcttt accgaaccgg
     6541 acgatcccga ccatttcatc ttcttcgaaa cgttccagcg cgtcacttaa tccggagcac
     6601 acgccgcgag gcaaatcgca ttgcgtgata tcaccgttga cgataaccgt cacgttctcc
     6661 ccgaggcggg ttaaaaacat tttcatttgc gcggcagtca cattctgcgc ctcgtcaaga
     6721 atgacgactg cattttcaaa ggtacgtcca cgcatatagg cgaacggcgc aatttccacc
     6781 ttccctattt ccggtcgcag gcagtactgc ataaaggaag cccctaagcg ccggaccagc
     6841 acgtcgtaga ccgggcgaaa atagggagca aacttttctg cgatatctcc aggtaagaag
     6901 ccaagatctt catcggcttg cagaactgga cgggtgacga taatcctgtc gacatcctta
     6961 tgtatcaggg cctctgccgc ttttgctgcg ctgatccagg tttttccgca cccggcttcg
     7021 cccgtggcga atatcagctg cttactctca atagccttca gatagtgcaa ttgcgcttca
     7081 tttcgcgcga ggatgggcga ggtatcgcga ctgtcgcggg ccataccaat ggcttctacg
     7141 ccgctcatct gcacaagcga ggtgaccgat tcttcttcac gctgcttatg gctgcgcgaa
     7201 tcccgtctca gcacacgttt tgcctcgcga cgagctttga tcactgcttt ttgtcttccc
     7261 atggagagca ccttgagttg tttgtattca tcacacgcgc cgttggcagc gcgattatgc
     7321 gcacgaacat cagagggttg gcttccttgt aagccatagt ttgcttttgg ataaaatgcc
     7381 gaaaaacggc tacgcgcacc gtttacggcg tcggtaacac atgaaaagaa aggatgaggt
     7441 tgaaaatgca aagtgacgag atgactaccg gaggagaaaa ctccgcgagt ggtggcgcgt
     7501 tgattatcta aaacatgtcc agtacaggac gttaccatcc gcgatctcca tagtgactga
     7561 ctatcactgc cgggaacttc cgctgctact taataagtac aacagatctc gcattcattg
     7621 caacaatata ttcacttata tttaactata aaacaccatt ttagtgacat tagtttctac
     7681 tggaaagatg acagagtgat gacagagtga tgacagagat gaaaaaagct gtgtgctttc
     7741 agcaggattc acatcaggca tcagtgcacg aacgtcggat gcggcgtgaa cgccttatcc
     7801 agcctaccaa acccgcaccg attgtgggca acataagcat cgcaagcgcc gcatcaggca
     7861 tctgcgcaca aatgccttat ccgcctaaaa acattaaacc cgcaataatg cgcttccgag
     7921 ataatcgttc gcgtccttca ctcccggcag cgcaaaaaaa taaccgccgc cgataggttt
     7981 aacgtattcc tccagcgctt cgccattgag ccttttttgt actgtcagga agccttttac
     8041 cagatcgtgc tggtagcaga caaacagcaa ccccatatcc agttgtccgg agttggtgac
     8101 gcccagtgaa taactgtagc cacgacgcag catcaggctg gactcactct ccgccgtgcg
     8161 gggattcgcc agccggatat ggctgtccag cgcgatcacc ttcccttccg ggtcgctggc
     8221 gtaatcaggc acatcatgct catgctgcat tcccagcggc gcaccggttt gcttatcacg
     8281 gccaaaaatc gtctgctgtt tatagcgcgg cgattttttt gataccgatt tgcatttcat
     8341 ttgataccga tgaaaaacgc cgatacgcaa cttatttgat actaaatcgg cgcttttctt
     8401 ttgagtttta gaatgagttt ttaagcgctc attcactctc tttcaaacca gttttaactg
     8461 tttgtggttt tgggtaaggc tggactttga atagctttgt gttgaggcgt tttctggccc
     8521 tcttgttcag aaaccggatg taacgaaact gtcggaactt atgcacactg gctctgtcga
     8581 tattagccct cagatgttca cctcgttgtc cgcctcgttt aatggcattc ctgcaaatct
     8641 cgtgatacca ctcgccatca agttcataga atattg
//
LOCUS       DAZFKF010000162         8591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFKF010000162 DAZFKF010000000
VERSION     DAZFKF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.164"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1852
                     /gene="espL4"
                     /locus_tag="ACHKA1_004840"
     CDS             <1..1852
                     /gene="espL4"
                     /locus_tag="ACHKA1_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFO5682385.1"
                     /translation="QRSDALDKQKLNLNSDRKEKEFPRVKLNGQCYFPGRPQNRIVCR
                     HIAAQYINDIYQNVNYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACG
                     DFGLVMKAYFDKMESNGISVMAAILLVDNHALTARLRIKNTTEGYTHYVVSVYDPNVT
                     NDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKG
                     SVDEITPPLSPATMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSINLPGLYL
                     AINYGNADIVETIFNSLSEPGYEGLLSKKNLMHILEAKDKNGFSGLFLAISRKDKNVV
                     TSILNALPKLAATHHLDNEQVYKFLSAKNSTSSHVLYHVMANGDADMLKIVLDALPLL
                     IRTCHLTKEQVLDLLKAKDFYGCPGLYLAMQNGHSDIVKVILEALPSLAQEINISASD
                     IVDLLTAKSLARDTGLFMAMQRGHMNVINTIFNALPTLFNTFKFDKKNMKPLLLANNS
                     NEYPGLFSAIQHKQQNVVETVYLALSDHARLFGFTAEDIMGFWQHKAPQKYSAFELAC
                     ELGHRVIAELIFNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(1821..3557)
                     /gene="aceK"
                     /locus_tag="ACHKA1_004841"
     CDS             complement(1821..3557)
                     /gene="aceK"
                     /locus_tag="ACHKA1_004841"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO5682386.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFYPEHGWEALLMRVLSDL
                     PLRLPWQNKSRDINYIIRHLTETLGPENFSKSHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDYGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRTLQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3741..5045)
                     /gene="aceA"
                     /locus_tag="ACHKA1_004842"
     CDS             complement(3741..5045)
                     /gene="aceA"
                     /locus_tag="ACHKA1_004842"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO5682387.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5075..6676)
                     /gene="aceB"
                     /locus_tag="ACHKA1_004843"
     CDS             complement(5075..6676)
                     /gene="aceB"
                     /locus_tag="ACHKA1_004843"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO5682388.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6945..7874)
                     /gene="metA"
                     /locus_tag="ACHKA1_004844"
     CDS             complement(6945..7874)
                     /gene="metA"
                     /locus_tag="ACHKA1_004844"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO5682389.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8031..8474
                     /locus_tag="ACHKA1_004845"
     CDS             8031..8474
                     /locus_tag="ACHKA1_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO5682390.1"
                     /translation="MVISIRRSQHEESEKLIAIWCRSVDATHDFLSAEYRTELEDLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TSNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2197 a         2095 c         2114 g         2185 t
ORIGIN      
        1 tcaacgctca gacgctcttg ataaacaaaa attaaatcta aactcagata gaaaagaaaa
       61 agaattccca agagttaaac ttaatggtca atgttatttt ccggggcgcc cacaaaaccg
      121 tatagtatgc cgacacattg ctgcacaata tattaatgat atttatcaga atgttaatta
      181 caaaccccat caagatgatt actcttcagc tgaaaaattt ctcacgcact tcaacaaaaa
      241 atgcaaaaac cagactttgg cgttgatttc cagccgtcct gaggggcgtt gtgttgctgc
      301 ctgcggtgat ttcgggctag ttatgaaggc atattttgac aagatggaat caaatggcat
      361 cagtgttatg gctgccatat tattggtaga taaccatgct ttgacggccc gactaagaat
      421 aaagaacaca actgaaggat atacccatta cgtggtttcg gtttatgatc caaatgtaac
      481 taacgataaa ataagaatca tgagcgaaag taaagaggat attaaacact attctctgat
      541 ggattttatg aatgtagatt atagcctcct gaaatggtca aatgatcatg ttattaacca
      601 atctgttgca ataattccag cacttccgaa agaacagcta ttgatgttaa aaggatctgt
      661 ggatgaaata acccctccat tatcacctgc aacgatgaat ttgctaatgg caattggtca
      721 gaatcaccaa cttacgcaac tgatgattca actccagaaa atgccagaac tacatagaac
      781 agaaatgttg actgcctata atagtattaa cctccccggt ttatatttgg ctataaatta
      841 tggtaatgcg gatatcgttg agactatttt taattcattg tcagagccag gatatgaagg
      901 attactctcg aaaaaaaatc tcatgcatat tctggaggca aaagataaaa atggtttttc
      961 tggattattt ttagcgatat cacgtaagga taaaaatgtt gtaacctcga ttctgaacgc
     1021 cttacctaaa ctggccgcaa cacatcattt agataacgaa caagtgtata aattcctgag
     1081 tgccaaaaat agtacgtcca gccatgtttt ataccatgtt atggcgaatg gtgatgccga
     1141 catgctgaaa attgttttgg acgcgttacc tttgttaatt cgcacatgtc atttgactaa
     1201 agaacaggta ctcgatctcc tgaaggcaaa ggatttttat ggttgcccag gactatacct
     1261 ggcgatgcaa aatggacata gcgatatcgt gaaagttatt cttgaagcat tgcccagcct
     1321 ggcccaggaa attaacattt cagcttccga tattgtagat cttctgaccg ctaaaagtct
     1381 tgcgcgcgac acgggtttgt ttatggccat gcagcgcgga catatgaacg ttattaatac
     1441 tatttttaac gcattaccca ctctgtttaa cacgtttaaa ttcgataaaa aaaatatgaa
     1501 gcccctcctc ctggcaaata attctaatga atatcccggt ttgttttcag cgatacagca
     1561 taaacaacaa aacgttgtag agacggttta tcttgcttta tctgaccatg cacgcctgtt
     1621 tggatttacc gctgaagata ttatgggttt ttggcaacac aaagcgccac aaaaatattc
     1681 tgcctttgag ttggcttgtg aattgggtca ccgggttatt gctgaattaa tctttaatac
     1741 attaaataag atggctgaaa gctttggctt tacggataac cctcgataca ttgcggagaa
     1801 aaattatatg gaagctttac tcaaaaaagc atctccccat accgtacgct aaatctttgc
     1861 ctgcgccgat acgcataaac atcttccaca tgtccttcac gaatgcggtt ttgtagtgtg
     1921 cgccagtaat cagcgcggaa caggtcggcg tgcatctctt caaagagtgg cccaatgcgg
     1981 gggtcggcgc atagccagtg gcggaactct tccgggaaaa catcgcccgg cgagacgctg
     2041 taccacggtt cgctggcaag ttcgtcttcc ggatagcgcg gcggcgggat gtcgcggaag
     2101 ttcacttccg tcatgtagca aatttcatcg taatcataaa aaaccacacg cccgtgacgg
     2161 gtgacaccga agtttttaaa cagcatgtcg ccagggaaaa tgttagcggc ggcaagctgg
     2221 cgaatagcgt taccgtattc ttcaatggcg tcacgcaact gctgaccttc cacttgttcc
     2281 agccagatat tgagcggcac catccgccgc tcaatataaa gatggcgaat cacaatttgt
     2341 tcgccgagat cggtgatttt ttccgctgct tcctggagca gtaattccat taatgccggg
     2401 gaaatatgcc gcttctccag cacaaagttt tcaaactcct gggtgtccgc cattcggccc
     2461 acgcgatcat gctctttcac cagttgatag caggcacgaa cgtgagcggc agacatctct
     2521 ttctgcggcg cgaacctgtc tttgatgact ttgaataccc gatcaaagcc cggtagcgta
     2581 aacaccaaca tcaccatgcc acgaataccc ggcgcttcaa tgaactgctc attacagccc
     2641 tgtagataaa cgagatattc gcggtagctc tcggttttgg catgcttctg gcagccgata
     2701 gccatataca attcagcggt ggttttacct ggcagaattt cccgtaacca ctcgaccagc
     2761 gctgcgggca gcggcgcata aaccataaaa taagaacgcg caaagccaaa aacaatgctc
     2821 gcttcggcgg tcgtcgtcag gcaggtatca ataaataact cgccgtagtc cgtctggtgg
     2881 atcggcagca aaaatggcaa tgtgccggaa ggcgtgatca gtttgcctac cagccaggcg
     2941 gctttattgc ggtaaaacag ttcgttcgcc acctgtaaat gacttttgga gaagttttct
     3001 ggccccaacg tttccgtcag atggcgaata atataattga tatcacggct tttattctgc
     3061 cagggcaggc gcagcggcag gtcactgagg actcgcatca gcagcgcttc ccagccatgc
     3121 tcggggtaga agtctttcgc cagtgggcgc ggaatggtac gaaagcggcg ctctggctga
     3181 gagctaaaga taaaaagccg ctcgggagta agcgagcggt ggtcaaataa ccgacagtac
     3241 acggagttaa aaaagctctc cgcaatctcg aagcgcgggt aatccggcaa cagccgggtg
     3301 taatgctctt tgacgcgcag taaaaattcc gcgtccgtgc tttggccgtt ggtaatgcag
     3361 cgcagttgct ccacgaccag accaacgtga tgatcgtaaa gatggatacg gtttttcatc
     3421 gcctgctgga cagcatgcca gtcggcctgt tcgaaacgct gctgcgcccc ggaagtcact
     3481 tcgaggaatc gaccatactg agcgtcgaag ccttgcaaaa tggtttgagc aatcagtaat
     3541 tccaggccac gcggcattta gcgccctcat caggagcaga gaattgccgg atgcggcgta
     3601 aacgccttat ccagcctacg ttcggcaacg gttgtaggca tgataagacg cgcaagcgtc
     3661 gcatcaggca tcgtgcactg attgccggat gcggcgtaaa tgccttatcc ggcctacagt
     3721 cagcaacgtt tgtaggttgc ttagaactgc gattcttcag tggagccggt cagcgcggtg
     3781 actgaagacg tgccgccctg aataatggtc gtcactttat cgaagtaacc tgtacccact
     3841 tcctgctggt gagatacgaa ggtgtagcca tctttcgcgg cggcgaattc cggttgctgc
     3901 actttctcaa cgtagtgctt cataccctcg ccctgggcat aggcgtttgc caggtcgaac
     3961 atgttgaacc acatgctgtg gatccccgcc agggtgatga actggaactt gtagcccata
     4021 tctgacagct gctgctggaa gctggcaata gttttgtcgt cgaggttttt ctgccagttg
     4081 aacgacggcg agcagttata agccagcagt ttgcccggat atttcgcgtg gatggcttgt
     4141 gcaaagcgac gcgccagttc cagatccggc gtggaggttt cacaccatac caggtcagca
     4201 tatggtgcat acgccaggcc acggctgatc gcttgctcaa tgcccgcatg agtacggaag
     4261 aagccttcac tggtacgctc gccggtaata aattcgctgt catacgggtc gcaatctgag
     4321 gtgatcagat ccgccgcatc agcatcggta cgtgcaacca gcagggttgg aacgcccgtc
     4381 acgtcagctg ccagacgcgc cgcgaccagt ttctgaatag cttcctgagt tggcaccaaa
     4441 actttgccgc ccatgtgacc gcatttcttc actgacgcca gctgatcttc gaagtgaact
     4501 gccgctgcac cggcttcaat catcgctttc ataagttcaa aggcattcag gacaccgcca
     4561 aaaccggctt ccgcgtcggc aacgatcggc aggaagtaat cgacatagcg cggatcgccc
     4621 ggctcaatgc ccgcggacca ttggatctga tccgcacggc ggaaagtgtt gttgatccgc
     4681 tccaccacag ctggcaccga gtttgccgga tagagcgact gatccggata catgctggcc
     4741 gccaggttag cgtccgccgc tacctgccat cccgacagat agactgcttc aatacccgct
     4801 ttcgcctgtt gcagcgcctg accgccagtc agtgcaccga ggctgttgat gtagcctttt
     4861 ttcgactcgc catgcagcag acgccacatt ttcgccgcgc ccagttgcgc cagcgtgcat
     4921 tcaggattga ctgaaccgcg taatttcacc acatcttccg cgctgtatgg gcgagtaatg
     4981 ccttcccaac gcggttgagt ccactctttc tgtaattctt caatttgttg tgtacgggtt
     5041 ttcatgtgca gatgctccat agttatgtgg tggtttacgc taacaggcgg tagcctggca
     5101 gggtcaggaa atcaattaac tcatcggaag tggtgatctg ttccatcaag cgtgcggcat
     5161 cgtcaaaacg cccctgggag aaacgttctt cgcccagttc gctggcaatg actttcatct
     5221 cttcgcccag catctggcgg aacaaggctt tggtcaccgg tttgccattg ctcaacgttt
     5281 tttgatgatg gatccactgc cagatcgagg tacgggaaat ttcagccgtc gccgcatctt
     5341 ccatcaggcc ataaatcggc acgcagccgt tgccggagat ccatgcttcg atgtactgca
     5401 cagccacgcg aatgttggcg cgcatacctt cttcggtgcg ttcaccatca caaggtgcca
     5461 gcagctgatc ggcagtaatc ggcgcgtctt gttcgcgcat cacttcaagc tgatttttac
     5521 gggagccgag aatgtcgtta aataccgcca ttgccgtatc cgcaaggcct gggtgagcga
     5581 tccatgtgcc atcgtgaccg ttattggctt ccagcgattt atccgctttt actttgttga
     5641 gcacttggtt attgcgctct tcatctttgc tcggaataaa cgccgccatg ccgcccatcg
     5701 caaaagcacc gcgtttatgg caggttttaa tcaacaggcg tgagtaagca ttcaggaatg
     5761 gtttatccat cgtcactgcc tgtctgtctg gcaggacgcg atcgggatag tttttcaacg
     5821 ttttgatata actgaagatg taatcccaac gaccgcagtt cagaccaaca atatggtcac
     5881 gcagtgcgtg aaggatttcg tccatctgga acacggcggg cagcgtttca atcagcaacg
     5941 tcgccttgat ggtgccgcgc ggcagattaa agcgatcttc tgcatagctg aagacttcgc
     6001 tccaccaggc cgcttcctgc caggactggg ttttcggcag atagaaatag ggaccgctgc
     6061 cctttgccaa cagtgcctga tagttgtgga agaaatagag cgcaaaatca aacaggctac
     6121 cggggattgc ctcaccacgc caggtgacat gtttttccgg caagtgcaga ccgcgtaccc
     6181 gacaaatcaa aaccgctgga ttgggcttga gctggtaaat tttgcctgct tcattcgtgt
     6241 aactgatggt gccgttaacc gcatcgcgca ggttaatttg cccgtcgatc actttgttcc
     6301 agtctggtgc cagtgaatct tcgaaatcgg ccataaagac tttcacattg gcgttcagtg
     6361 cgttgatcac catcttgcgc tctaccgggc cagttatctc tacgcggcgg tcttctaagt
     6421 ccgcaggaat cccgcgaatt ttccaatcag catcgcgaat ggaagctgtt tccgaaataa
     6481 aatcaggcaa cgttccgtta tcaatatctt gctgctgctg aatgcgcgct gccagaagtt
     6541 tattgcgttg tggcgtaaaa tgcgtcacca gctcagtcag aaattctacc gcttcggcag
     6601 taagaatttg cttctcctgc tcgccatacg gccttgtgaa agccagttca tcggttgttg
     6661 ttgcctgttc agtcatcgtg cagctcctcg tcatggatcc gaaaacttcc ccactgtgaa
     6721 cgaaggatcg ttgtgcgctt ttcgttcagc acaactaaga ctactcattt aaaatgcaaa
     6781 atcaaaaaca atttccattt taattaaaaa tacttgataa cttattgata acaatatgaa
     6841 taaaatttat tcacctactg aggtgtgttt cggaaataaa aaaggcaccc gaaggtgcct
     6901 gaggtaaggt gctgaatcgc ttaacgatcg actatcacag aagattaatc cagcgttgga
     6961 ttcatgtgcc gtagatcgta tggcgtgatc tggtagacgt aatagttgag ccagttggta
     7021 aacagcaaat taccgtgact acgccagctc gctcgcggtg tattttgcgg atcattgtgc
     7081 gggaaatagt tatacggtac atccgggtct agtccggctt ccacatcgcg gaaaaattcc
     7141 tgcgccagcg tttgcgcatc atattcggga tggcccgtca caaaggcaat gcgcttatct
     7201 ttgctggcaa acagatatgc atccccttct tccgtctctg ccagaatttc cagatcggta
     7261 taatcacgaa tcaacgctgc cggaaagtca gcatagcgcg aatgtggtgc caggaatgaa
     7321 tcatcaaagc cacgcgtcag aagcgcatga ggatggagaa tatgatgctc gtaaacgcca
     7381 gagagtttgt cggtgcgagt ttgcttaggt atgccgtaga ggatattgag cgcggcctgt
     7441 accgcccagc agacaaacag cgtcgaggtg acgtgatctt tcgaccactc cagcacctgt
     7501 ttgatctgcg gccagtaagc gacatcatta aactccacca ggcccagcgg cgcaccggtt
     7561 acaatcagac cgtcaaagtt ctgttcctga atatcttcaa agttacagta gaagttgttc
     7621 agatgctctg cgggcgtgtt gcgcgattca cgggaatcga tgcgcaacag ctgaatatcg
     7681 acctgcaaag gtgagtttga aagcaggcgc agaaactgat tttcagtttc aatcttcttc
     7741 ggcatcaggt taaggatcag aaccttaagt ggacgaattt cctgaccaga cgcacgagaa
     7801 gttgtcatca caaagacgtt ttcttcacgc aagaaattga cggcgggtag ctcgtccggc
     7861 acacgaatcg gcataacctg attacctcac tacatacgct tatacgttta gacatccaga
     7921 tagctgaaga taaccagaaa atttgccaat gtcgagcctg catgttgaag gtgagaaagt
     7981 ttcaaggaaa agactgcgtt agaatataag aatgactaaa aggagagaaa atggttatta
     8041 gtattcgccg ctcgcagcat gaagaaagtg aaaaactgat tgcgatttgg tgtcgttctg
     8101 tcgatgccac tcacgatttt ttatcagcag agtatcggac cgagctggag gacctagttc
     8161 gttcctttct gccggaagca ccgttgtggg tcgcggttaa tgaacgggat cagccggttg
     8221 ggtttatgtt gttaagtggg cagcatatgg atgcgctgtt tatcgatcct gatgtgcgcg
     8281 gctgcggcgt gggccggatg ctggtgaagc atgcgctctc aatggccccg gaactgacaa
     8341 gcaacgttaa tgagcaaaat gagcaggcgg ttgggttcta taagaaggtg ggttttaagg
     8401 ttacgggacg ctctgaggtg gacgatttgg ggaaaccgta tccgttgctg aatctggcgt
     8461 atgtggggga ataagctgcc ccctatcgtg ctatcgtgcc tgcgtaagct ggataagacg
     8521 cgccagcgtc gcatccggca tttttttgca ccaacggcaa aaaggccatc cgtcaggatg
     8581 gcctttcgct t
//
LOCUS       DAZFKF010000163         8425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFKF010000163 DAZFKF010000000
VERSION     DAZFKF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.165"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(187..263)
                     /locus_tag="ACHKA1_004846"
     tRNA            complement(187..263)
                     /locus_tag="ACHKA1_004846"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Asp,seq:gtc)
     gene            complement(396..1127)
                     /gene="dnaQ"
                     /locus_tag="ACHKA1_004847"
     CDS             complement(396..1127)
                     /gene="dnaQ"
                     /locus_tag="ACHKA1_004847"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO5682391.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1192..1659
                     /gene="rnhA"
                     /locus_tag="ACHKA1_004848"
     CDS             1192..1659
                     /gene="rnhA"
                     /locus_tag="ACHKA1_004848"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO5682392.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1656..2378)
                     /locus_tag="ACHKA1_004849"
     CDS             complement(1656..2378)
                     /locus_tag="ACHKA1_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5682393.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2412..3167
                     /gene="gloB"
                     /locus_tag="ACHKA1_004850"
     CDS             2412..3167
                     /gene="gloB"
                     /locus_tag="ACHKA1_004850"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO5682394.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3239..4597
                     /gene="mltD"
                     /locus_tag="ACHKA1_004851"
     CDS             3239..4597
                     /gene="mltD"
                     /locus_tag="ACHKA1_004851"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO5682395.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4645..5268)
                     /locus_tag="ACHKA1_004852"
     CDS             complement(4645..5268)
                     /locus_tag="ACHKA1_004852"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5682396.1"
                     /translation="MSGLPQGRPTFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKN
                     ITTRHGYAESLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQTVEALRDTSHVRNYASCEWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQNDGSFTSDIIMVEAHKAA"
     gene            complement(5272..6072)
                     /locus_tag="ACHKA1_004853"
     CDS             complement(5272..6072)
                     /locus_tag="ACHKA1_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO5682397.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6313..7227
                     /gene="yafC"
                     /locus_tag="ACHKA1_004854"
     CDS             6313..7227
                     /gene="yafC"
                     /locus_tag="ACHKA1_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO5682398.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGALTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7224..8027)
                     /gene="dkgB"
                     /locus_tag="ACHKA1_004855"
     CDS             complement(7224..8027)
                     /gene="dkgB"
                     /locus_tag="ACHKA1_004855"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO5682399.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVSRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8190..8266)
                     /locus_tag="ACHKA1_004856"
     tRNA            complement(8190..8266)
                     /locus_tag="ACHKA1_004856"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8230..8232),aa:Asp,seq:gtc)
     gene            complement(8321..>8425)
                     /gene="rrf"
                     /locus_tag="ACHKA1_004857"
     rRNA            complement(8321..>8425)
                     /gene="rrf"
                     /locus_tag="ACHKA1_004857"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2259 a         2021 c         2046 g         2099 t
ORIGIN      
        1 ggaaatatat ttaagctgaa tggttcgata tcattattga attttaatga atgagacaac
       61 aagcgagaat cacgcactta cgccagagta aaatcattga acagagaaat ggtggaattc
      121 aaatacaaaa aaaccgcaaa attaaaaatc ttgcggttct ctgaactcat tttcatgagt
      181 gaatagtggc ggaacggacg ggactcgaac ccgcgacccc ctgcgtgaca ggcaggtatt
      241 ctaaccgact gaactaccgc tccgcgttgt gttccgttgg gaacgaggcg aatattacgg
      301 attgcctcac ccttcgtcaa cggtttttct catcttttga atcatttgct gcaaaaatcg
      361 cccaagtcgc tatttttagc gccttttaca tgtatttatg cccgccagag gcagcttccg
      421 ccctttttct gcaccagatc gagacgggct tcatgtgctg caagctcttc atctgtcgca
      481 aaaacaacgc gtaacttact tgcctgacgt acaaggcgct ggattgttgc ttcaccttgt
      541 tgctgttgtg tctctccttc catcgcaaaa gccatcgacg tttgaccacc ggtcatcgcc
      601 agataaactt ccgcaaggat ctgggcatcg agtaatgccc cgtgcagcgt tcgtttactg
      661 ttatctattt cgtagcgagc acataacgca tcgaggctgt tgcgcttacc gggaaacatt
      721 ttcctcgcca ccgcaaggct atcggtgacc ttacagaagg tattggtctt cggaatatcg
      781 cgcttaagca acgaaaactc gtagtccata aagccgatat cgaacgctgc gttatggatc
      841 accaactccg cgccgcggat atagtccatg aactcatcgg ctacttcggc aaacgtgggc
      901 ttatcgagca aaaattcatc ggcaatacca tgtacaccaa aggcttccgg atccaccagc
      961 cgatcgggtt tgagataaac atggaagtta ttgcccgtca ggcgacggtt caccacttca
     1021 acggcaccaa tctcaatgat cttgtggcct tcatagtgcg caccaatctg gttcataccg
     1081 gtggtttcgg tatcgagaac gatctggcgt gtaattgcag tgctcatagc ggtcatttat
     1141 gtcagacttg tcgttttaca gttcgattca attacaggaa gtctaccaga gatgcttaaa
     1201 caggtagaaa ttttcaccga tggttcgtgt ctgggtaatc ctggacctgg gggttacggc
     1261 gctattttac gctatcgcgg acgcgagaaa acctttagcg caggctacac ccgcaccacc
     1321 aacaaccgta tggagttgat ggccgctatt gtcgcgctgg aggcgttaaa agaacattgc
     1381 gaagtcattt tgagtaccga cagccagtat gtccgccagg gtatcaccca gtggatccat
     1441 aactggaaaa aacgtggctg gaaaacagca gacaaaaaac cagttaaaaa tgtcgatctc
     1501 tggcaacgtc ttgatgctgc gctggggcag catcaaatca aatgggaatg ggttaaaggc
     1561 catgccggac acccggaaaa cgaacgctgt gatgaactag ctcgtgccgc ggcgatgaat
     1621 cccacactgg aagatacggg ctaccaagtc gaagtttaag cctgtggttt acgacattgc
     1681 cgggtggctc caaccgcctg gcgaattcgt ggcttgtttt tactctgttt catcggattt
     1741 agcgttaaag gaatagtccg tttccgggca acaataagtt gtaagcaacc aagcgcagga
     1801 atatgcgcat tcaaaagttt tcctccgtgt ttgttccacg ggagaacgtg gaaacggctg
     1861 gcgtgtagca cttcaaaatt caacaaagag agccagtcca gctgccgcat cagagtaaac
     1921 atccggctgt tatagggcga ggttttgcgc aataccggca caagtttgcg taatcccatt
     1981 aaactaatgg gattgaagcc actaatcacc agccagccat catcaatcaa tacccgatcg
     2041 gcttcacgca ataaacgatg cggatcggtg caccacggca atgtatgtgc cagtagacaa
     2101 acatcaacgg atttatcggc aaaaggaaga tgaagtggat ccgcctgcac ctggacgggc
     2161 attccttgcg cagaaacatt cacttgatga gaaaccgcgc acgcttcgca attgatttct
     2221 gcgcttaaat tgccaatctt aagcagatga aaaccataca ttttagtgaa ccacgggttg
     2281 agctggcgct ccagcgcctt gcgataaagc tctccccagg gcaaatcgcc ccagcgatca
     2341 ggagccacga cagtttgagg gactcttgcc ggtttcatca caaccttccg tttcacactg
     2401 agaggtaatc tatgaatctt aacagtattc ccgcctttga tgacaattac atctgggttt
     2461 tgaatgatga agcaggtcgc tgcctgattg tcgatcccgg agacgcagag ccagtattaa
     2521 acgccattgc cgccaataac tggcaaccgg aggccatatt tctcacccac catcatcacg
     2581 atcacgttgg cggcgtaaaa gaactggtgg aaaagtttcc gcaaattgtg gtgtatggtc
     2641 cacaagagac acaagataag ggaacaacac aggtagtcaa agatggcgaa actgccttcg
     2701 ttttggggca tgaatttagt gtaattgcca caccgggtca cactttagga catatctgtt
     2761 acttcagtaa accttatcta ttttgcggcg acacgctgtt ttctggtggg tgtggtcggt
     2821 tgtttgaagg gacagcatca caaatgtatc aatcacttaa aaagttaagt gcgttacctg
     2881 acgatacatt ggtatgttgt gctcatgaat atacgttatc aaatatgaag tttgctttga
     2941 gtattcttcc gcacgatttg tccataaatg attattatcg taaagttaag gagttacggg
     3001 caaaaaatca aataacacta cccgtaattc tgaaaaatga gcggcaaatt aatgtttttt
     3061 taagaacaga agatattgat ttaattaatg taattaatga agaaacatta ttgcaacaac
     3121 ctgaagaacg ttttgcatgg ttaaggtcaa agaaagatag gttctgataa aactttcttg
     3181 tcatcggctc cgttcgccgt tatgatcggt cgtcttttaa gcaactattg acacacacat
     3241 gaaggcaaaa gcgatattac tcgcctctgt cctgctcgtg ggttgccaga gtaccggcaa
     3301 cgttcaacag cacgcacaga gcctttctgc agctggtcaa ggggaagcag caaagtttac
     3361 aagtcaggca cgatggatgg acgatgggac gtctatcgcg ccagatggtg acttgtgggc
     3421 tttcattggc gacgagctaa agatgggaat tccggaaaat gaccggattc gcgaacagaa
     3481 acagaaatat ttacgcaata agagctatct ccacgatgta actttacggg cagagccgta
     3541 tatgtactgg atagccgggc aagttaaaaa acgtaacatg cctatggaac tggtactact
     3601 acccatagtg gagagcgctt ttgatcctca cgcaacgtct ggcgccaatg ccgcaggcat
     3661 ctggcagatc attccgagca cggggcgcaa ttatggtttg aaacagaccc gcaattatga
     3721 tgcgcgtcgc gatgttgttg cttcaacaac tgccgcgctg aatatgatgc agcgtctgaa
     3781 caaaatgttt gacggcgact ggcttctgac cgtagcggct tataacagcg gcgaaggtcg
     3841 ggtcatgaag gcaattaaaa cgaacaaagc gcgtgggaaa tccacggact tctggtcgtt
     3901 accgttgccg caggaaacga agcagtacgt gcctaaaatg ctggcattga gtgatattct
     3961 caaaaacagc aagcgttatg gcgtacgtct gccaacgacc gatgaaagcc gtgctctggc
     4021 gcgtgtgcac ctgagcagcc cggttgaaat ggcgaaggtt gcagatatgg cggggatttc
     4081 cgtcagcaag ctgaagacat tcaacgctgg cgtgaaaggc tccacgctgg gcgcaagtgg
     4141 tccgcaatac gtgatggtgc caaagaagca tgcagatcaa ctgcgtgaat ctctggcttc
     4201 aggcgaaatt gctgctgtac agtcgacgct ggttgccgac aatacgccgc ttaacagccg
     4261 tgtttacacc gtacgctctg gcgacacgct ttcaagtatc gcttcacgtc tcggcgtaag
     4321 caccaaagat ttgcagcagt ggaacaaact gcgcggatct aagctgaagc caggccaaag
     4381 tttgacgatt ggtgcaggca gtagcgcaca gcgactggca aacaacagcg atagcattac
     4441 gtatcgtgtg cgcaaaggcg attcgctttc aagcattgct aaacgccacg gcgtgaacat
     4501 caaagatgtg atgcgctgga acagcgacac tgcgaatctg caaccaggcg ataagctgac
     4561 gttgtttgtg aaaaacaaca acatgccaga ttcctgacaa accagataat aaaaaggcac
     4621 cgattccccc ggtgcctttt ttatttatgc cgctttatgt gcttctacca tgatgatatc
     4681 actggtgaaa gagccatcat tctgcaagga aaaatacgtt ctcacctctg tcgatgcgct
     4741 ctgttggtaa atgcgaatag cgtctactaa cgcttctggc gtacgcattc tcacgaccca
     4801 tgaagaaaat tccagcggta acttatctgt aattaaatta tcaactatca gattggcttc
     4861 attgattaac gtcaaccact cacagctggc gtagtttcgt acgtgagagg tatcgcgtaa
     4921 tgcttctacc gtctgtaacc agatgtcgcg cactgggtgg cccggagaca ttacgtccat
     4981 cacaatcagc ctaccgccag gtttcaatat cctattcact tctcgcagtg ctgcgccgac
     5041 atcatgccag tgatgggcag aataacggct gataacaata tcaaatgcgt tatcggcaaa
     5101 tggcagactt tcggcataac catgacgagt agtgatattt ttcagttgcc gggcttcggc
     5161 agcttgtgcc acgacatcca gcatttgggc agataagtca tacgccacca ccgcgctcac
     5221 gttttgcgca gcgacaaacg ttggtctgcc ctgtggcaga cctgacatac cttatttatc
     5281 aggcttgccg ggactgaatt caacgagtag cggattgtga tcggaagcgc gcgtaaccag
     5341 tacagaagct tcactgacgt tcagaccacg gtagaaaaca aaatcgagcg ggcgaccaaa
     5401 cgcccgacgg cgctgatcat cggtaaaacg cacctgacgc agcgacattt cccgcgcaaa
     5461 acgatataac gcgttcatcc ttctacggct ccaggcattg aaatctcccg ccataatgac
     5521 cgggccgctg tggtgagcta tctgatcacc aataggaagt aactgcttac tataaacatc
     5581 cacgcccaga ctgaagttga cggcgtgtat attaaccacc atcaacaggc gggtgtcagg
     5641 taatggatag accgtcacca gtgccgactt cgccagacgc aaaatgggtt ctcgttcacg
     5701 taacgggcag caatacactg gatgtgccgc cgaaagggtc attacgccag aaggatgttg
     5761 tggcagcacg aaagcgggta cctgatcggc ggcaagatag ttagcagtcg caaactgtac
     5821 caactctggc gttgtttgcg cttcctgcaa taacaccaga tgtgcgtctt tgccgtaatt
     5881 ctttaatacc gacaaccatt cagcgcgttg ctgtttgtat atgttccaca ccaggatccg
     5941 aatacgctct tcggtactta acggttctcc gggtggtaat gcctggccga tgctcgcaaa
     6001 agaccccggc ggtaagatcc tctccgcagg ttgtccggca acatagcgca tggcataggt
     6061 gttttttcgc acttttgact ttgaaacctc tattacgtca acccttccgc actcgggagg
     6121 gttctactgt tatagggatt ttagctcact ctttcaacga gcaattgtat attgttatgt
     6181 aagcaagtgc ttacaccggt cagaattttg ctcattattg agaacattaa atcggatgac
     6241 atatcacgca tcctgatggt ttaatgacaa ataaattcaa aatatctcat ctattttgca
     6301 acaggagcaa aaatgaaagc cacgtcggaa gaactcgcca tttttgtttc ggtcgtcgaa
     6361 agcggcagct ttagccgggc agcggaacaa ttagggcaag caaactcagc ggtaagccgg
     6421 gcggtgaaaa agctggagat gaaacttggc gttagcctgc ttaatcggac cacgcggcaa
     6481 cttagcctga cggaagaagg cgagcgttat tttcgtcgcg tacagtcaat tttgcaggag
     6541 atggcagcgg cagaatcaga aattatggag acgcgtaata caccgcgtgg actgttgcgc
     6601 atcgatgccg caactccagt ggtgctgcac tttctgatgc cgttgattaa gcctttccgt
     6661 gaacgctatc cggaagtcac tttatcgcta gtctcctccg aaacgattat taatttgatc
     6721 gaaagaaaag tggatgtcgc gatacgcgct ggtgcgttaa cggattccag cttacgtgcc
     6781 aggccgttat ttaacagtta tcgaaaaatt atcgcctccc ccgattatat ttcccgctac
     6841 gggaagccag aaacgatcga cgatttaaag caacatgttt gcctgggatt cactgaaccc
     6901 gcttccctca atacctggcc gatagcctgt agcgatggac aattacatga ggtgaagtac
     6961 ggtttgtcat ccaatagtgg ggaaacactg aaacagcttt gcctgagtgg caacgggatt
     7021 gcgtgtttgt ccgactatat gatcgacaga gaaatcgctc gcggagaatt ggtggagtta
     7081 atggcagata aagtgttgcc agtggaaatg ccattcagtg cagtctatta cagcgaccgt
     7141 gcggtaagta cgcgcatccg ggcttttatc gatttcctta gcgagcatgt aaaaacagct
     7201 cccggaggag ctgtcagaga ggcttaatcc cattcaggag ccagaccttc cgggctaacc
     7261 aggcggtcgt tgcaatccag tgcggcgatc gcttttttat cttcggcatc aagctgtaaa
     7321 ttttgtgcct taagattact ttccaggttt ttacgtttag tagaagaagg aattactgag
     7381 taaccttccc ccatagccca cgccagaatc acttgtgccg gagtcgcatt gtgtttagct
     7441 gcgatacgag caataacctc atctttcagg gccttaccat acgccagcgt catataggaa
     7501 gtaatatgga tgccgtgctg tttagcccag gcaaccactt tacggttttg cagataagga
     7561 gagagttcaa tctggttagt agcgatgttt tcagcgccaa cagcagcaat cgccttttcc
     7621 atcaatggga tcgtgaagtt ggaaataccg atctcacgcg tcaacccttc ttttttggct
     7681 tccagcagcg cctgcataaa ctcttcaaca gagacttcat cgtttggtga cggccagtgg
     7741 attagagtca gatcaacata atcagtacgc aatttttgca ggctctcttt cagactcggg
     7801 atcaatttgt ctttgctgag attttcaatc cagattttag tggtgatgta gagttcatga
     7861 cgtgacacgc cactttctgc aatcgcctga cctacggcag cttcgttatc atagatttgt
     7921 gcggtatcaa ttgcgcgata accaagttca agcgccgttt tcacagatga aataacaacg
     7981 tcgtctttca gacggaaagt acctaaacca aatgcaggga tagccataat atgcctcttt
     8041 taatgctatt ttcgttaact gaggtgatta tgggcttcag actgttgcgg aaaaagagcg
     8101 taaaacgcag aggatttttg cgattttagc aaaaatagaa atgtagacga aaaaaaacct
     8161 cgagcgataa ctcgaggctt cttaataagt ggcggaacgg acgggactcg aacccgcgac
     8221 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     8281 caaccaccgg ttttatgacc ggcttactgc ttaatttgat gcctggcagt tccctactct
     8341 cgcatgggga gaccccacac taccatcggc gctacggcgt ttcacttctg agttcggcat
     8401 ggggtcaggt gggaccaccg cgcta
//
LOCUS       DAZFKF010000164         8390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFKF010000164 DAZFKF010000000
VERSION     DAZFKF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.166"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..344
                     /locus_tag="ACHKA1_004858"
     CDS             <1..344
                     /locus_tag="ACHKA1_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682400.1"
                     /translation="RSDDVSPLFIEIEDNLRTFNTNELTRNDNIFILFSSVDDGPVMV
                     VSSQRLHDMLNPTKDTNWNSTYIYKSRHEILPVNLTPETLFGSKSQDKHALFPIFTAS
                     WRANRIKNKGI"
     gene            567..1064
                     /gene="ubiC"
                     /locus_tag="ACHKA1_004859"
     CDS             567..1064
                     /gene="ubiC"
                     /locus_tag="ACHKA1_004859"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO5682401.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1077..1949
                     /gene="ubiA"
                     /locus_tag="ACHKA1_004860"
     CDS             1077..1949
                     /gene="ubiA"
                     /locus_tag="ACHKA1_004860"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO5682402.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2104..4527)
                     /gene="plsB"
                     /locus_tag="ACHKA1_004861"
     CDS             complement(2104..4527)
                     /gene="plsB"
                     /locus_tag="ACHKA1_004861"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO5682403.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPTHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4698..5066
                     /gene="dgkA"
                     /locus_tag="ACHKA1_004862"
     CDS             4698..5066
                     /gene="dgkA"
                     /locus_tag="ACHKA1_004862"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO5682404.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5176..5784
                     /gene="lexA"
                     /locus_tag="ACHKA1_004863"
     CDS             5176..5784
                     /gene="lexA"
                     /locus_tag="ACHKA1_004863"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO5682405.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5857..7182
                     /gene="dinF"
                     /locus_tag="ACHKA1_004864"
     CDS             5857..7182
                     /gene="dinF"
                     /locus_tag="ACHKA1_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO5682406.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLFGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFSAT"
     gene            7298..7507
                     /gene="yjbJ"
                     /locus_tag="ACHKA1_004865"
     CDS             7298..7507
                     /gene="yjbJ"
                     /locus_tag="ACHKA1_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO5682407.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7549..8064)
                     /gene="zur"
                     /locus_tag="ACHKA1_004866"
     CDS             complement(7549..8064)
                     /gene="zur"
                     /locus_tag="ACHKA1_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO5682408.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1926 a         2033 c         2268 g         2163 t
ORIGIN      
        1 ttcgtagtga tgatgtttca cctttattta tagaaattga agataattta agaaccttta
       61 acacgaatga attaacacga aacgataata tctttatctt gttctcctct gtcgatgatg
      121 ggccagttat ggtggtaagc tcccagcgct tacatgatat gttgaatcct acaaaagata
      181 ccaattggaa ttccacgtat atctacaaat ccagacatga gatattgcct gttaatctta
      241 ctccggaaac acttttcggc tccaaatctc aggataaaca tgcacttttc cccattttta
      301 ctgcgagttg gcgagctaat cgtataaaga ataaaggtat ttaagtaaag gaaaacatca
      361 ccgttcctgg catcctgggc ggtgatgccc ctatggttgc cctcgccagc acgggcatcg
      421 gcaaagcgta aggttcaaca gcgttttacc acttcatgcg attgttgcgt ttttgttgcg
      481 cattagatca cttaatttgc tttacatctc ccgtaaacac ttttctgcga tacaatgcct
      541 ttacgttatg taacggagag ttcggcatgt cacaccccgc gttaacgcaa ctgcgtgcgc
      601 tgcgctattt taaagagatc cctgccctgg agccgcaact gctcgactgg ctgttgctgg
      661 aagattccat gacaaaacgt tttgaacagc agggaaaaac ggtgagcgtg acgatgatcc
      721 gcgaagggtt tgtcgagcag aatgaaattc ccgaagaact gccgctgctg ccgaaagagt
      781 ctcgttactg gttacgtgaa attttgttat gtgccgatgg tgaaccgtgg cttgccggtc
      841 gtaccgtcgt tcctgtgtca acgttaagcg ggccggagct ggcgttacaa aaattgggta
      901 aaacgccgtt aggacgctat ctgttcacat catcgacatt aacccgggac tttattgaga
      961 taggccgtga tgccggactg tgggggcgac gttcccgcct gcgattaagc ggtaaaccgc
     1021 tgttgctaac agaactgttt ttaccggcgt caccgttgta ctaagaggaa aaaaatatgg
     1081 agtggagtct gacgcagaat aagctgctgg cgtttcatcg cttaatgcgt acggataagc
     1141 caattggcgc gttactgctg ctctggccaa cattatgggc gttgtgggtg gcgacaccgg
     1201 gcgttcccca gctctggatc ctggcggtgt ttgtcgcggg tgtctggctg atgcgcgctg
     1261 ccggatgtgt ggtgaatgat tatgctgacc gcaagtttga tggtcatgtt aagcgcacgg
     1321 cgaaccgacc acttcccagc ggcgcggtaa cagagaaaga ggcgcgcgcg ctgtttgtag
     1381 tgctggtact gatttcgttt ttactggtgc tgacgctgaa tacgatgacc attctgttgt
     1441 cgattgccgc gctagcgctg gcgtgggtgt acccgtttat gaagcggtat acccatctac
     1501 cgcaagtggt gctgggcgcg gcgtttggct ggtcgattcc aatggctttt gctgctgtga
     1561 gtgagtcggt gccattgagt tgctggttaa tgttcctcgc caatattctc tgggcggtgg
     1621 cttacgacac gcagtatgcg atggttgacc gtgatgatga tgtgaagatt ggcattaaat
     1681 ccacggcaat cctgttcggc caatacgata aattgattat tggtattttg cagattggcg
     1741 tactggcact gatggcgatc atcggtgagt taaatggctt aggctgggga tattactggt
     1801 caattctggt ggctggcgcg ctgtttgttt atcaacaaaa actgattgcc aaccgcgagc
     1861 gtgaagcctg ctttaaagca tttatgaata ataactatgt tggtctggta ctgtttttag
     1921 ggctggcaat gagttactgg catttctgat gatgtaaaaa agccggatga tcatccggct
     1981 ttcttctggg ttgcctgatg cgctgcgctt ctcaggccta cacaacagat cgcaatttat
     2041 tgaatttgca gattatggaa ggccggataa ggcgtttgcg ccgcatccgg caattctctc
     2101 tgattaccct tcgccctgcg ttgcactctc aatcgtcaaa cgcacgtctg atgtaatcaa
     2161 ctccgccagc aactgataaa ccttcatcgt ttctgccggt tcggcatcgc cgctatcgct
     2221 gatataccct tcatcacgta gcgtcagcac cagagaactg aacaccgcct tgtcgaagaa
     2281 ctccggcgcg ttgataccgt gcagcacgga gagacgttgc gcgacggtgc ggctctcttt
     2341 ctccagcgta ccgcggttga tcgacgggtt ggcgctcaac agccagaagg tgatagcata
     2401 acgttgcagc gtttcgcgcg cgcctgcggc cagcagctgt agcgtgcgag aatgcgtcgg
     2461 gttgatatgc aactcatcat cttgcagggt aatcagcccc tgacgttgca tctcatttgc
     2521 cagcgcatca ataacgtccg gcaactcgtc gcgatcccag cgcaggaaca gctccgcttt
     2581 cagcattggg taaagcacat tgacgtgctc catcaatacg tcgcgggaga tgtggcgatg
     2641 ctgggtgaca attgccgcca tcagcgaagg cagcaccaac atatgcgcaa tgttgttgcg
     2701 atagtaggtc atcagcaccg cttgctcgcg cggcagaatg atgatgtcgc cgatagtgtc
     2761 tttctcaact tcaaacttgt tcatttgcag cgcgtgatcg ataagctcgc tggcgctggc
     2821 tgaaggaacg gtagagtccg tggagtaagg cacgttgcgc atcagatcca ggtagcagtt
     2881 gagttgctcg gttaactgct cgcgggtgag tgagcgctga cgtgatgcca gtagcgcagt
     2941 acagcacagg ttcatggcgt ttgccgcgcc tgcgttgtta atgcgtacca tcagatcggc
     3001 agcaatatta ttgaccgtcg gtgttaacca tgccggacgc accgcttcga tgggatcgat
     3061 agattcacgc cagtcaggta cgtgctggtt aaggtaggtc atcaacggca ttggttcacc
     3121 gaaattgacg taaccctgac cgagattacg cagcttgctt aaaccgcgca gcatctgcgg
     3181 caggctctct ttctctttcg tcgcgccgcg cagttctttg gcgtaagtac ccacttccat
     3241 gacgtgctca taaccgatat agatcggaat cagcgtaatc ggacgcgtgc cgccacgcag
     3301 catcgcctga atggtcatcg acagcgtacc ggttttcgga tccagcaaac gccccgtacg
     3361 ggaacgaccg ccttctacga agtactcgac ggaataacca cggctgaaca gttcgccgag
     3421 atactcacgg aaaacggtgg aatagagctt attgccttta aaagtacggc gaataaagaa
     3481 cgcacccaga cggcggaaaa tcggcccggc cggccagaag ttcaggttga tcccggcggc
     3541 gatatgcggc ggcaccagcc cctggtgata cagcacgtaa gaaagcagca ggtagtccat
     3601 gtgactgcgg tggcaaggca catacaccag ctcatgaccg tcgtgggcca actggcgaac
     3661 gcgctcggcg ttatggacgt tgataccctg gtaaagtcgg ttccaggtga agcccagaat
     3721 acggtcagtc aggcgaatca tctcgtaaga gaaattcgcc gcaatctctt ccatcagtgc
     3781 aatcgcgttc tgctgcgctt tttcatggga gatttttttg ctgcgcgctt catcttctac
     3841 cgctttcgca atggcgcggg aggcgagcag cttattaaac agatcctgac gagcaggcaa
     3901 acgtgggcct acggcagcca gacgttgacg ggcaaagtgc atacgcgcca cgcgcgccag
     3961 tttctgcgcg atagttttat ccgtgccgtg ttcatccgcc atacggcgca gcgaaacgga
     4021 cggtgagaaa cgcacaaaac tgtcgcgacc gagccacagt acagcgaaaa atttctgtac
     4081 gccgttaagc atacgcagcg gcgggttcac ttcgcctttt tcacgccccg gcgcgcgacc
     4141 aaacatcacc gacactggca ccatctgcac atccagattt gggttgctac ggtgcaaatc
     4201 gagatagtcg tggaacagct taatagactc ttctttcggc gtgtaatagg tgaatacacg
     4261 cggcccgccg tgaatgaaca catagcgcgg cagtagcgtg ccgtcgattt ccagcggctc
     4321 taacgggtca ggcaagtcat gtgccagaca ctgggcgcgc aacgtcagca agtctgcttt
     4381 cgagttgtac ggtaaaacgt acataattgg acgagaggta tccagcccca gttccggggc
     4441 aggatctgcc ggaatagact tgctttttac caggatgctt aatggtaaat tcagtaattt
     4501 gtagtaaatt cgtggccagc cggacataaa cgatgtaaag cctctggtta ataatgcaaa
     4561 tgcgcggcaa ggatagcaga aagtcatggg aaattctgtg gtatccgctc atgtttcgcg
     4621 cggcgttacg caaacccgaa tcatcggatt taacggtaca ctgatattga cgctcataat
     4681 gtaaaaaggt tctttcaatg gccaataata ccactggatt cacccgaata atcaaagctg
     4741 ctggctattc ctggaaaggt ttacgcgctg catggatcaa cgaagcggca ttccgtcagg
     4801 aaggcgtagc ggtattgttg gcggtggtca tcgcctgctg gctggatgtg gacgcgatta
     4861 cccgcgtgct gcttatcagc tccgtgatgc tggtgatgat tgtggaaatc ctcaatagcg
     4921 ccatcgaagc agtggttgac cgaattggct ctgaatacca tgagctttcc ggacgcgcta
     4981 aagatatggg atccgccgcg gtgctgattg cgattatcgt cgccgtgatt acctggtgca
     5041 tcctgttatg gtcgcatttt ggataaccct tccagaattc gataaatctc tggtttattg
     5101 tgcagtttat ggttccaaaa tcgccttttg ctgtatatac tcacagcata actgtatata
     5161 cacccagggg gcggaatgaa agcgttaacg gccaggcaac aagaggtgtt tgatctcatc
     5221 cgtgatcaca tcagccagac aggtatgccg ccgacgcgtg cggaaatcgc gcagcgtttg
     5281 gggttccgtt ccccaaacgc ggctgaagaa catctgaagg cgctggcacg caaaggcgta
     5341 attgaaattg tttccggcgc atcacgcggg attcgtctgt tgcaggaaga ggaagaaggg
     5401 ttgccactgg taggtcgtgt ggctgccggt gaaccgcttc tggcgcagca gcatattgaa
     5461 ggtcattatc aggtcgatcc ttccttgttc aagccgaatg ctgatttcct gctgcgcgtc
     5521 agcgggatgt cgatgaaaga tatcggcatt atggatggcg acttgctggc agtgcataaa
     5581 actcaggatg tacgtaacgg tcaggtcgtt gtcgcacgta ttgatgacga agttaccgtt
     5641 aagcgcctga aaaaacaggg caataaagtc gaactgttgc cagaaaatag cgagtttaaa
     5701 ccaattgtcg ttgaccttcg tcagcagagc ttcaccattg aagggctggc ggttggcgtt
     5761 attcgcaacg gcgactggct gtaacatatc tctgagaccg cgatgccgcc tggcgtcgcg
     5821 gtttgttttt catctctctt catcaggctt gtctgcatgg cattcctcac ttcatctgat
     5881 aaagcacttt ggcatctcgc cttacccatg attttctcca atatcaccgt tccgttgctg
     5941 ggactggtcg atacggcggt tattggtcat cttgatagcc cggtttattt gggcggcgtg
     6001 gcggttggtg caacggcaac cagctttctc tttatgctgt tgctgttttt acgcatgagc
     6061 accaccgggc tgactgcgca ggcttatggt gccaaaaatc ctcaggcatt agcccgtgcg
     6121 ctggtgcaac cgttgctgtt ggcgttgggg gctggggcgt taattgcgct gctgcgtaca
     6181 ccgattatcg atctggcgct gcatattgtt ggcggtagtg aagcagtcct ggaacaggcg
     6241 cggcgctttc ttgaaatccg ctggttaagc gcaccggcgt cgttggcgaa tctggtatta
     6301 ctcggttggt tactcggcgt gcaatatgcc cgtgcgccag taattttgtt agtggtcggc
     6361 aatatcctca acattgtgct ggatgtctgg ctggtaatgg ggctgcatat gaacgtgcag
     6421 ggtgcggcgc tggcgacggt tattgcggaa tatgcaacat tgctgtttgg tctgctaatg
     6481 gtgcgtaaaa tcctcaaact acgcggaatt tccggcgaaa tgctgaaaac tgcctggcga
     6541 ggaaacttcc gtcgcttgct ggcgcttaac cgcgatatca tgctgcgttc gctgttgttg
     6601 caactctgtt tcggcgcgat caccgtactt ggtgcgcgac tggggagtga cattatcgct
     6661 gttaacgcgg ttctgatgac gctactcacc tttaccgcct atgcgctgga tggttttgcc
     6721 tacgcggttg aagcacattc cggtcaggca tacggtgcgc gcgacggtag ccagttgctg
     6781 gatgtctggc gggcagcgtg ccgccagtcg gggatcgtag cgttactgtt ttcggtggtt
     6841 tatttgctgg caggggaaca catcattgcg ttgctgacgt cgttaaccca gattcagcag
     6901 ctggccgacc gctatcttat ctggcaggtg attttgccgt tggttggcgt ctggtgttat
     6961 ctgctggacg gcatgtttat aggcgcaacg cgtgccgccg aaatgcgtaa cagtatggcg
     7021 gtggccgccg caggttttgc gctgacgctc cttacgctgc cgtggctggg caatcatggt
     7081 ttgtggctgg cgttaaccgt ctttctggcg ttgcgcgggc tttctctggc ggctatctgg
     7141 cggcgtcact ggcgcaatgg tacctggttt tccgcaacgt gacggttaaa aattctgaat
     7201 aaataatcct aagccaaatt gctgactaca cttaatctca cgttcagaag aaaagtgaac
     7261 gtactctcat tcacaaccta acgatgaggt cttgattatg aataaagatg aagccggcgg
     7321 taactggaaa cagtttaaag gtaaagtgaa agagcaatgg ggcaaactga ccgatgatga
     7381 tatgacgatc attgaaggta aacgtgatca actggtcggt aaaatccagg aacgttatgg
     7441 ttatcagaaa gatcaggcag aaaaagaggt cgtggattgg gaaacccgca atgaatatcg
     7501 ctggtaatta atccctcctg cccgacgtgt acaaggatgt acgccctctt aacgcggttt
     7561 ctttttcacc tgcacagagt gatcatgctg gcactgttca ggatgacgac acgcttccac
     7621 ttccacgcat gccgcacaga gcccatgtgc ttcaatcaca ttatgccgca gggcaaaccc
     7681 catttttgcc gccagcgtat gcatgatgtc ttccacgcct tctgcacatt cttctttcac
     7741 cgcgccgcat cgatcgcaaa taaacatagc tgacgtatgg gtgggctggt cgaacagatg
     7801 acagagcaca taactgttgg tggattccac cttatgcaca aaaccttgct caagcagaaa
     7861 atccagcgcg cgataaaccg ttggcggctt ggcttgcggt tcagcttcgc gcagcaaatc
     7921 aagcagatcg taagcgctga tagcgccatc ctgcagactc atcaggcgca acacttccag
     7981 gcgctgtggg gtcaggcgca cattacgctg cgcgcagatt ttttcagcct gcgctaataa
     8041 ctcctgcgtt gtggtctttt ccatcgggca cctcaaagtg ggggttaaga atgtccttac
     8101 tttaccatgt tccaggaaaa acaccgagaa cccttattgt tgtcgtaatg ttgattttct
     8161 gttttgtacg taaggtgttg ttttgcctgg tcgtaccatt atcaacacga taataattaa
     8221 taattcattt ttaaataaat tgcttcaagt taacttgcta tttatttaac cgtggctaat
     8281 cgtatacccg aatttcagaa aattatgagc gatttttgat agtgaccggt tccaaaaaca
     8341 cttataagga ggacacggta cgacaaaaaa ggtagatgaa tcatgttaaa
//
LOCUS       DAZFKF010000165         8351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFKF010000165 DAZFKF010000000
VERSION     DAZFKF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.167"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..696)
                     /locus_tag="ACHKA1_004867"
     CDS             complement(<1..696)
                     /locus_tag="ACHKA1_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682409.1"
                     /translation="MPNTSIHLSRCNILQNNKLQPAEIYKESQQTAKLEFFCDEFLKI
                     SQSRYGLSTSTDSIANLLAFFTKASDAIDSIKTQKIDVHSYGFVPIRHFVEHVITYKN
                     EFAADYQPYTLTFTRGENNEGILSVESKEGFISQRTINLNEYETAINIINEHVIKENI
                     HNTVQSPTEKDISKINSSDKPYKTSSKESIKSQLYSDQKNIQTCYYILRKNTEWYKYA
                     SSEERYDKFKNSSK"
     gene            complement(1160..2038)
                     /locus_tag="ACHKA1_004868"
     CDS             complement(1160..2038)
                     /locus_tag="ACHKA1_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5682410.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VNILLRDRRKAIQLTTAGHRIVQHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPVVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIISKGRYELSIMALFKEKNITPQIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKTIADELCSVPLEPTFYRQISLLAKEKPVEGSPL
                     FLLQMCTEQLVVSGKI"
     gene            2190..3380
                     /locus_tag="ACHKA1_004869"
     CDS             2190..3380
                     /locus_tag="ACHKA1_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5682411.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLLFFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            3461..4111
                     /gene="inaA"
                     /locus_tag="ACHKA1_004870"
     CDS             3461..4111
                     /gene="inaA"
                     /locus_tag="ACHKA1_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO5682412.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIGGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(4165..4419)
                     /gene="yfaE"
                     /locus_tag="ACHKA1_004871"
     CDS             complement(4165..4419)
                     /gene="yfaE"
                     /locus_tag="ACHKA1_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO5682413.1"
                     /translation="MARVILRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4419..5549)
                     /gene="nrdB"
                     /locus_tag="ACHKA1_004872"
     CDS             complement(4419..5549)
                     /gene="nrdB"
                     /locus_tag="ACHKA1_004872"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO5682414.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(5783..8068)
                     /gene="nrdA"
                     /locus_tag="ACHKA1_004873"
     CDS             complement(5783..8068)
                     /gene="nrdA"
                     /locus_tag="ACHKA1_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO5682415.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            8196..8339
                     /gene="ypaB"
                     /locus_tag="ACHKA1_004874"
     CDS             8196..8339
                     /gene="ypaB"
                     /locus_tag="ACHKA1_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO5682416.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         2007 a         1918 c         2103 g         2323 t
ORIGIN      
        1 cttagaagaa ttcttgaatt tatcatagcg ttcctcggag gatgcgtatt tgtaccattc
       61 tgtatttttt ctcagaatgt agtagcaggt ctgtatattt ttttgatcgc tgtaaagttg
      121 acttttaata ctttccttac tgctggtttt ataaggttta tcagagctgt ttatttttga
      181 aatgtctttt tctgtcggtg actggaccgt gttgtggata ttttctttta taacatgttc
      241 attgatgatg ttaattgcag tttcatattc atttaagttt attgttcttt gtgaaataaa
      301 cccttctttg ctttcgacac tcaaaatacc ttcgttgttt tcacctcggg taaaggtaag
      361 ggtatagggt tgataatccg ctgcaaattc atttttatac gttatgacat gttcgacaaa
      421 atgtcttatt ggtacgaaac cataactgtg aacgtcaatt ttttgggtct tgatactatc
      481 aattgcatct gaggctttcg tgaaaaaagc caataaatta gctatgctgt ctgttgaggt
      541 tgataacccg tatctacttt gagatatttt tagaaactca tcgcaaaaaa actcaagttt
      601 ggcggtttgt tgtgattctt tgtaaatttc tgctggttgc aatttattat tttgtaaaat
      661 attacagcga gataagtgaa tagatgtatt tggcataaat gattccttgt attgtgagtt
      721 aacaagatac aatttaatct ttgcagaata tattcactat caaatttagc tcattttatt
      781 atgaagacct ttacgcatgt tagaaagtat cattattgtt aataaatacg gtaataagta
      841 acaaacagat attgataact tccctgacaa gttattaggt atcccacttg aagattgaga
      901 atggctccag agtaaactgt gtactgttcg ccggagtgtc attactctca acaatcggat
      961 ttgacagtga gtcgggcgct tcataccggt ttctacctgg agtcattgtc acttctccca
     1021 actggataaa tatatcgcca attcacaaag tgggccaaaa tcaggtgaaa acgaattatt
     1081 acgcctggta aaggctatag tacaactaag tgtggcgaat caggcagtct tgcatttgtc
     1141 atcaatctca tgtcttacat caaatttttc cactcaccac tagttgttcc gtacacattt
     1201 gtagtaaaaa cagtggactg ccttctaccg gcttttcttt agccagcaac gaaatctgtc
     1261 gatagaaggt tggttcgaga ggaacggaac acaattcatc tgcaatggtt ttcagcgtta
     1321 actctggtaa taaggcgatg cctaatccct gtcgaataaa actgatcgct gtatccgggt
     1381 gattaaattc atattttatc tgtggggtaa tattcttttc tttaaatagt gccataatgc
     1441 ttaattcata gcgtccttta ctaatgatta atggttcacc cattaattct tccactgtaa
     1501 tagtgctgtt ttttgccaga gcatggttgg gcggtaacac tacggtgaat ttatcctgat
     1561 aaatgggcac ggagtacatt ccgctcaccg gaaaatgaac aaaaccggca tcaatggatt
     1621 cattctgaag cgaatcaata atcgctgtgc tattttcctc aaaaggaata atttttatat
     1681 tcggatgatg aatttcaaag tagcgaatca caaccggtaa gatacaggcg cagacgctgg
     1741 ggaaacaacc aagtctcagc gttctttgag ggttctgctt ttcctgctcg gctatctctt
     1801 ttactgcgtt aacttcccga ttaatggcgc gcagatgttg cacaatgcgg tgacctgctg
     1861 tggtcagttg tatagcttta cggcgatcgc gtaacagtat attcacacca agttcttgtt
     1921 cgagggcagc aattgcctga cttaccgccg actgcgtcat gaacaatcgc ttgctggctt
     1981 cagtaaagcc accgtaatcc agcacggcga gtaaagaatt tatttgggta agagtcatta
     2041 gagtttgcta atcaataata ttaaaacgat taatttctct aatagcatgc cgccatttat
     2101 acttctgttc tgcaaagtac atcacaattt gacgcaaccg cagtaacggg agtgttgtct
     2161 gcgtgcgtca tcctgacagg cggtagggta tgaaagagaa gttatggacg aaggattttt
     2221 gggcaataac catcatcagc tttattattt tcttcgtctt ttatgtttta ctaacattgt
     2281 tgccaattta tatctctgac cgcttgcatg cctctcctga taaagcaggt ttgttggtga
     2341 cattattttt aattgcggcg attgttattc gaccctttgc cggacaatgg gtgggtaaat
     2401 attcgaataa aactattctg gtgctctctt ctctggcctt tttggtggtc actgcgctgt
     2461 atcctttttg ccactcaata gaatcactgc tttttattag ggtgcttcat ggtattacct
     2521 tcggggttat cacaacggta aagggaacga tttccgcgcg gctgatcccg gcctcccgac
     2581 gtggggaggg catcagtttt ttctctctgg caatggggct ggcaatggtg gtcgggccgt
     2641 ggattggcct gaatatggcg cgctgggagg cctttaatat ggctttctgg ttatgtactg
     2701 gcgttgcggc ggtggggatt atcctgtcgc tgattatgac cgtgccgccg gttatcagcc
     2761 atgccgatgg ttcaaagcca aagatgggct tcgccgccat gttcgatcgc gcggcattgc
     2821 cgtttgccat ggttacattc tttatgacct tttcgtatgc cggggtttct gcctttctgg
     2881 cgctttacgc ccgcgaactt aatctcatgt cggcggccag taatttcctg ctctgctacg
     2941 ccatcttcct gatgatctgc cgtaccttca ccggcaatgt ttgcgacaaa aaaggcccga
     3001 aatatgtggt ttacccctgc ctgctgttct ttacggttgg gctggtggtt ctcggctaca
     3061 cccagggcag cgtaatgatg gtcgtttctg gcgcgttgat tggtatcggg tatggttccg
     3121 tgacgccagt ttttcagacg cagattatca gttcagtgga accgcataaa atcggtgtcg
     3181 caaactccct cttcttcaat gcgatggatg caggcctggc gctgggagcc tgtgtgatgg
     3241 ggatgatggt tgcacatact ggctaccgaa tgatttatct gctgggcgca ctattagtgg
     3301 tagtggctgg tggagtctat gcgctgcaaa tgaagggtaa aagcggtgtc gcgctagtag
     3361 tggcaaaaga aattcattaa tacgacacgt ttcattaaga tttttcgcag gtaaccacct
     3421 atagtcattg tcgttaataa aaaatgacag gcgatagggt atggcagttt cagcaaagca
     3481 cgacgaattt aatcactggt gggcaacaga gggcgactgg gttgaagagc ctaactatcg
     3541 ccgtaatggt atgagcgggg tgcaatgcgt cgagcgcaac ggcaaaaagc tatatgtaaa
     3601 gcgcatgacg catcatctgt ttcattccgt acgttatcct ttcggccgac caacgattgt
     3661 ccgtgaggtt gcggtaatta aagaactgga acgggccggt gtcatcgtgc cgaagatcgt
     3721 ttttggtgaa gcggtgaaaa ttgggggtga atggcgcgcg ttgctggtga ctgaagatat
     3781 ggcggggttc atcagcattg ctgactggta tgcccagcat gcagtaacgc cctattctga
     3841 cgaagtacgg caagccatgt tgaaagcagt ggcgctggcg tttaagaaaa tgcatagcgt
     3901 taatcgtcag catggctgtt gttatgttcg ccatatctat gtgaaaacag aaggtaaggc
     3961 agaagctggt tttcttgatc tggagaaaag tcgtcgccgc ttgcgtcgcg ataaagcgat
     4021 taatcatgac ttccgccagc tggaaaaata tctggagccg atccccaaag cggactggga
     4081 gcaggtaaaa gcgtattact acgccatgta atcgttgtag gccagataat acgcataagc
     4141 gttgtatctg gcactacaac caattcacat ctcgatttca atatcgcctt ttgcccggca
     4201 acaacagggc aaaatttccc ccggctgaat aaaggctaac ggttcggcaa tccagtccac
     4261 ctggcctgca acaaggcgcg tgcgacagga gccgcagtaa ccttcgcggc actggtactc
     4321 aaccgccaca ttgtgggatt ccagcgccgc cagaagggaa gggtgttcat cctggcacag
     4381 cagttgtgtg ccagtgatgc gcagaataac gcgggccatc agagctggaa gttactcaaa
     4441 tcgtcggtgt ccacttccga gtcaatctgc ccgaccagat aagaactcac ctccacttcc
     4501 tgtggagcaa cctgcacgtt atcagacacc agccaggtgt tgatccacgg gatcgggttg
     4561 gagcgcgtct ggaacggcag atccaaacca accgcctgca tacggatatt ggtgatgtac
     4621 tccacgtact ggcagagaat gtctttgttc agaccaatca tcgaaccgtc acggaacaga
     4681 taatccgccc agtctttctc ctgttgtgcc gcctggacaa acaggtcata gcactcctgc
     4741 ttacattctt cggcaatttc cgccatctcc ggatcatccg cgccgctgcg cagcagattc
     4801 agcatatgct gggtgccggt caggtgcagg gcttcgtcgc gggcaatcag gcgaataatt
     4861 ttggcgttac cttccatcag ctcacgttca gcgaatgcga aagagcaagc aaagctgacg
     4921 tagaaacgaa tcgcttccag cgcgttaacg ctcatcaggc agagatacag ttttttcttc
     4981 aactcgcgca ggctaacggt tacggtttta ccgttaacgg tgtgggtgcc ttcgcccagc
     5041 aggtgccagt agctggtcat ttcgatcagc tcatcgtaat agctggagat cccttccgca
     5101 cgtttctgga tctgctcgtt ggtgacgata tcgtcaaaca caacagacgg atcgttaacg
     5161 atattacgaa tgatatgagt ataggaacgg gaatgaatcg tttctgagaa cgcccaggtt
     5221 tcgacccagg tttccagttc cggaatagaa ataagcggca atagcgccac gttcgggcta
     5281 cgaccctgaa tggagtccag cagcgtctga tatttcaggt tgctgataaa gatgtgtttt
     5341 tcgtgttcag gcagcgcctg gtaatcgata cggtcgcggg agacgtcaac ttcttccgga
     5401 cgccagaaga aagagagctg cttttcgatc agcttttcga agatgtcata tttttgctga
     5461 tcgtagcgag ccacgttgac cggctgacca aagaacatcg gttctttgag ctgatcattt
     5521 ttcgtctgtg aaaaggtggt atatgccatg agtgtgtcct gttgggagtt taatgccgga
     5581 taaggcattt tacgccgcat cctgtgcctg atgcgacgct ggcgcgtctt atcaggccta
     5641 caaatccgag ccgtaggccg gataaggcgt tcacgctgca tccggcaccc ggagcctgat
     5701 gcgacgctgg cgcgtcttat caggcctaca aaccgagccg taggccggat aaggcgttta
     5761 cgccgcatcc ggcatctcaa tatcagatct tacatgcgcc gctttcgcag ccatcgtcct
     5821 ggattgacgg caccagatcg tcctgcgcgt cttcagcgcc gtcacgggtg ttctgataat
     5881 acagcgtttt gaccccgaat ttgtaggcgg tgagcaggtc tttcagcaat tgctgcatcg
     5941 gcacttttcc tgacgggaag cgtgacggat cgtagttggt gttagcagag atcgactgat
     6001 cgataaattt ctgcatgata cccaccagtt gcaggtagcc atcgttaccc ggcatttccc
     6061 acagcagctc ataggcgtcg tgcagatgct cgtagtccgg cactacctgg cgcagaatac
     6121 cgtctttcga cgctttgatg ctgacgtaac cgcgcggcgg ttcaataccg ttagtggcgt
     6181 tagagatctg tgaagaagtc tcggacggca tcagggcaga aagcgtggag ttacgcagac
     6241 cgtgcgtttt gatcgattca cgcagtgctt cccagtcgta atgcagtggt tcattagcga
     6301 tggcatccag atctttctta taggtatcga tcggcaggat ccctttcgcg taagtggttt
     6361 cgttaaacca cgggcacgcg ccttgctctt tcgccagctc attagacgct ttcagcaggt
     6421 aatactgaat cgcttcgaag gttttatgcg tcaggttgtt ggcgctgccg tcggagtagc
     6481 gtttaccatg cttcgccagg tagtaagcga agttgatcac accaataccc agcgtacgac
     6541 gacccatcgc tccacgtttg gcggccggga tcgggtaatc ctgataatcc agcagcgcgt
     6601 caagtgcacg aaccgccaga attgccagct cttccagttc atccaggcta ttaattgcgc
     6661 ccaggttgaa agcagacagc gtacacagcg cgatttcacc gttctcgtcg ttgacgtcgt
     6721 tcagcggttt ggtcggcagg gctatctcca ggcacaggtt agactgacgc actggcgcga
     6781 tggccggatc aaacgggcta tgggtattgc agtggtcaac gttctgaata tagatacgac
     6841 cggtagatgc acgttcctgc atcatcagcg agaacagctc aacggctttc acacgctgct
     6901 tgcggatgct gtcgtctttc tcatatttgg tatacaggcg ttcaaactct tcctgatcgg
     6961 cgaagaatgc gtcgtacagc cccggtacgt cggacgggct gaacagggtg atatcttcac
     7021 ctttcagcag gcgggtatac atcagtttgt tgatctgtac cccgtagtcc atatggcgca
     7081 cgcggttgcc ttccacacca cggttgtttt tcaacaccag caggctttcc acttccagat
     7141 gccacatcgg gtagaacagc gttgccgcac ctccgcgcac gccgccctga gagcaggatt
     7201 tcaccgcagt ctggaaatgt ttgtagaacg gaatgcagcc ggtatggaac gcttcgccac
     7261 cgcgaatcgg gctacccagc gcacgaatac gcccggcgtt gatgccgatc ccggcacgct
     7321 gggaaacata tttcacaatc gcgctggagg tggcgttgat ggaatccagg ctgtcgccgc
     7381 actcgatcag tacgcaggag ctgaactgac gagtcggggt acgcacgccg gacatgattg
     7441 gcgtcggcag cgaaatttta aatgtggaaa ccgcgtcgta aaaacgcttc acgtattgca
     7501 ggcgcgtttc acgcgggtag ttcgagaaca ggcacgcagc aaccagaata taaaggaact
     7561 gggcgctttc gtagatttcg ccggtcacac ggttctgtac cagatatttg ccttccagct
     7621 gcttaacggc agcataagag aaggtcatat cacggtcgtg atcgataaac gtgtccatct
     7681 gcttgaactc ttcttccgtg tagtcttcca gcagatgatt atcgtatttg cccatctcga
     7741 ccattttcac cacatggtca tacagcgcag gtggctcaaa ctggccgtag gcttttttac
     7801 gcaggtggaa gatcgccagg cgcgcggcga gatactgata atccggcgca tcacgggaga
     7861 tcaggtctgc ggcagccttg ataatggttt cgtggatgtc agaggtcttg ataccgtcat
     7921 aaaactgaat gtgggagcgc agctcgacct gggaaatcga aacgttatgc agtccttctg
     7981 ccgcccaatc cagaacgcga tggattttgt cgagattgat gcgctctgtg ctaccgtcgc
     8041 gctttgtcac cagcagattc tgattcatgt atgtcgtacc tgtttttgga attctttccg
     8101 caatagtttc atgcagacta cctgtagtac gggaagctgc ttagaacact atatataggg
     8161 ggtatgtttg ggattcactg caagatagtg tgaaaatggc cctcttgcaa gtgcataact
     8221 ttgtggataa ctcaggaagg aaaaagtggc tttcgcgcac cttaggtcag acaaggtgtc
     8281 cgggaaagtc aatgggaaga gaaaaatttg ttaaaaataa ctgttcggcg atttcttgaa
     8341 cggcataata g
//
LOCUS       DAZFKF010000166         8218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFKF010000166 DAZFKF010000000
VERSION     DAZFKF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.168"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            185..1060
                     /gene="rluB"
                     /locus_tag="ACHKA1_004875"
     CDS             185..1060
                     /gene="rluB"
                     /locus_tag="ACHKA1_004875"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO5682417.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(1100..1690)
                     /gene="cobO"
                     /locus_tag="ACHKA1_004876"
     CDS             complement(1100..1690)
                     /gene="cobO"
                     /locus_tag="ACHKA1_004876"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO5682418.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVLQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(1687..2445)
                     /locus_tag="ACHKA1_004877"
     CDS             complement(1687..2445)
                     /locus_tag="ACHKA1_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO5682419.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2665..3714
                     /gene="sohB"
                     /locus_tag="ACHKA1_004878"
     CDS             2665..3714
                     /gene="sohB"
                     /locus_tag="ACHKA1_004878"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO5682420.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(3750..4001)
                     /locus_tag="ACHKA1_004879"
     CDS             complement(3750..4001)
                     /locus_tag="ACHKA1_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO5682421.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            4381..6978
                     /gene="topA"
                     /locus_tag="ACHKA1_004880"
     CDS             4381..6978
                     /gene="topA"
                     /locus_tag="ACHKA1_004880"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO5682422.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKHQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            7188..8162
                     /gene="cysB"
                     /locus_tag="ACHKA1_004881"
     CDS             7188..8162
                     /gene="cysB"
                     /locus_tag="ACHKA1_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO5682423.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         2097 a         1949 c         2185 g         1987 t
ORIGIN      
        1 taaccataaa gatagtgtag tatgttgcgc ctcaaagcta ggccagttaa aacgtcgagt
       61 cgtttactta aggcctgaag agttcaaaca gactttacta tcagtgagtc agatgtaagt
      121 tctgactcac tgttgcacaa aagcatatca gacgcctggg aaggcgacac caaaaggaag
      181 ctctatgagc gaaaagctac agaaagtgct ggcgcgtgcc ggccacggtt ctcgccgtga
      241 aatcgaatct attattgaag ccggtcgtgt gagtgttgat ggcaaaattg ctaaactcgg
      301 cgatcgcgtt gaagttaccc ctggattgaa aatccgtatc gatggtcacc tgatttcggt
      361 acgtgagtcc gctgaacaaa tttgtcgcgt tctggcctat tacaaaccgg aaggtgagtt
      421 gtgtacccgt aacgatccgg aaggacgtcc aacggtgttt gaccgtctgc caaaactgcg
      481 tggcgcacgc tggattgccg tgggtcgctt ggacgttaat acctgtggtc tgctgctgtt
      541 caccaccgat ggtgaactgg caaaccgttt aatgcaccca agccgtgaag ttgaacgtga
      601 atatgccgtg cgtgtatttg gtcaggttga cgacgcgaaa ctgcgtgatt tgagtcgtgg
      661 cgtgcagttg gaagatggtc cggcagcttt taaaaccatc aagttcagcg gcggcgaagg
      721 gatcaaccag tggtacaacg tgactctgac cgaaggccgt aaccgtgaag ttcgtcgtct
      781 gtgggaagcg gttggtgtgc aggtgagccg cctgatccgt gttcgttacg gtgatatccc
      841 actgccaaaa ggtctgcctc gcggtggctg gacggagctg gacctcgccc agactaacta
      901 tctgcgcgaa ctggtggagc taccgccaga aaccagctct aaagtcgctg tagaaaaaga
      961 ccgtcgtcgc atgaaggcga atcagattcg tcgtgcggtg aaacgtcaca gtcaggtgag
     1021 cggcggtcgt cgttctggcg gacgtaataa taacggttaa tcaaagtatg ccggacgtca
     1081 tatccggcat ttttacagat taataatcga tccctatctg cgcttttaca ccggcatcaa
     1141 acgcatgttt gatggggcgc aactcactca ccgtatccgc cagttcaaga atatcccgat
     1201 gacagcctcg acccgtgata atcaccgtct gttgatgtgg acgttcattt aacgcctgca
     1261 acacttcttc cagtggcaaa tagtcatacg ccaccatata tgtcagctca tcgagcaaaa
     1321 ccatatccag tgaggaatca gcaagcatcc gctttgcatg ttgccagact tcgcggcagg
     1381 cggcggtatc agactcgcgg ttttgtgtat cccaggtaaa gcccgttgcc atcacctgaa
     1441 actcaacgcc atgtggctcc agcagattgc gttcgccatt aggccaggtg cctttaataa
     1501 actgcacgac gcccactttt tttccgtgac caactgcgcg tgttgccgta ccaaatgccg
     1561 cggtggtttt gccttttcca ttgccggtaa agacgatgat aataccgcgt tcatcctggg
     1621 cctgggccac acgagcatct actttttctt tcactcgctg ctgacgctgt tggtagcgtt
     1681 catcactcat tgggaaattc ctggtttacg gcccggttgg gcgtcaaagg tcatgccggt
     1741 tttacggcgg ctgtcatcgc ccatcagcca gaggtagagc ggcatgatat cagcgggagt
     1801 tttaagtttc tgtggatctt cggtcgggaa ggcgctggca cgcattgcgg tgcgcgtacc
     1861 gcctgggtta atgcagttga cgcgcaggcg ctgctgatat tcatcggcca gtacctgcat
     1921 catcccttcg gtggcaaatt tcgacgctgc atatgcgccc cagttggctc gtccctgacg
     1981 tccaacgctt gatgaagtaa agaccagtga accggcgtcc gatttgagta ataaaggaag
     2041 cagtgcctgg gtgagcataa aggtggcatt aacgttgacc tgtatgacgt cctgccagac
     2101 ctgtggattt tgttcgctca ttggacaaac atcgccgagc aatccggcat tatgcaaaac
     2161 accatccaga cgcggataat taacggcaat gcgctgtgcc agttgttggc aatcttcgga
     2221 cctgcaggtc agcaaatcga gaataaacca ctgtggctga cgcccagttt cttcgtttat
     2281 gtggctggct acctgacgta atttttcttc attacggccc aacagaatca ctgtcgcacc
     2341 atagcgtgca tacgtcatcg cggcttcacg accaatacca tcactggctc ccgtcaccag
     2401 gataatgcga tcattgagta aatcttgttt tggctggtaa tgcatggcta ctcctcaacg
     2461 acgttgtctg tcgcgctgcc agtgtacttt atgactttgg ggctttatgc ctgaaacaga
     2521 agggcatttc aatcagcctg gggcgataac accgcaaaat ggatactttg tcatactttc
     2581 gctgcaataa catctctgcg agacggctta acatgcctgt tgtaaactgt gagccaaagc
     2641 gttgtttaac caaggtgggg actcgtggaa ttgttgtctg aatatggttt gtttttggcg
     2701 aaaatcgtta ccgttgtgct agcgattgcg gcgattgccg ccattattgt caatgttgct
     2761 caacgtaata aacgccagcg tggcgagtta cgggtcaaca atctcagcga acagtataag
     2821 gagatgaaag aagaactggc cgcggcgctg atggactcac atcagcaaaa acagtggcac
     2881 aaagcgcaga agaaaaagca taagcaagaa gcgaaagcag caaaagcgaa agccaaactg
     2941 ggcgaggtgg caactgacag taaaccccgc gtctgggtgc tggattttaa aggcagcatg
     3001 gacgcccatg aagtgaactc gctacgtgaa gagataacgg ctgtactcgc agcattcaaa
     3061 ccgcaggatc aggttgtgct acgtctggaa agccctggtg gcatggtgca tggttacggg
     3121 ttggcggctt cgcagctgca gcgtctgcgt gataaaaaca ttcctttaac tgttacggta
     3181 gacaaagtcg ctgccagcgg cggttacatg atggcctgtg tggcggacaa aattgtttcc
     3241 gcaccgtttg ctattgtggg ttccattggt gtggtggcgc aaatgcccaa ctttaaccgc
     3301 ttcctgaaaa gcaaagatat tgatatcgaa ctgcacaccg ccgggcagta taagcgcacg
     3361 ctgaccttgc tgggtgaaaa taccgaagaa gggcgggaga aattccgcga agagctgaac
     3421 gaaacgcatc agttatttaa agattttgtg aagcgtatgc gtccgtctct ggatattgaa
     3481 caggtggcaa cgggtgaaca ctggtacgga caacaggcgg tagagaaagg cctggttgat
     3541 gaaatcaaca ccagtgatga agttattctt agcctgatgg aaggccgtga agtggtcaat
     3601 gtacgctata tgcagcgtaa acgactcatt gaccgattca ccggcagcgc ggcagagagc
     3661 gccgatagat tattgctacg ctggtggcag cgtgggcaaa agccattgat gtaaaagaca
     3721 aacgcgaggg taagacctcg cgttttgctt taatcaacca gatgatattt ttctgaaagc
     3781 acatgggcca ggtgtttgaa catattaaac accgcggtgc ttttggctgt tggcaatcct
     3841 tgttcatcta aaaagtagtc gccggtaaat accagcacgc cattacgctg cgtgacgccg
     3901 gtggcttcaa tccctgcgag cgtatcctca tgctcacgaa tgattttgtt ggcctctttc
     3961 aacagcgttt cgcggtcgat gggttgtgtc tctttgttca ttatttactc cttaaacaag
     4021 gacattagtc tacgccaggc atggcttgca gacaaatata ccacgctggt ggcaagagcg
     4081 ccttactggc aactttggat tttgcatgct aataaagttg cgtatcggat tttatcaggt
     4141 acagtgtgac gctttcgtca atctggcaat agatttgctt gacattcgac caaaattccg
     4201 tcgtgctata gcgcctgtag gccaagacct gttaactcag tcacctgaat tttcgtgaac
     4261 agagtcacga caaggggttg atatccgcag agagcgagtc catatcggta actcgttgcc
     4321 agtggaaggt ttatcaacgt gcgacgcatt cctggaagaa tcaaattagg taaggtgaat
     4381 atgggtaaag ctcttgtcat cgttgagtcc ccggcaaaag ccaaaacgat caacaagtat
     4441 ctgggtagtg actacgtggt taaatccagc gtcggtcaca tccgcgattt gccgaccagt
     4501 ggctcagctg ccaaaaagag tgccgactct acctccacca agacggctaa aaagcctaaa
     4561 aaggatgaac gtggcgctct cgtcaaccgt atgggggttg acccgtggca caattgggag
     4621 gcgcactatg aagtgttgcc tggtaaagag aaggtcgtct ctgaactgaa acaactggct
     4681 gaaaaagccg accacatcta tctcgcaacc gaccttgacc gcgaagggga agccattgca
     4741 tggcacctgc gggaagtgat tggtggtgat gatgcgcgct atagccgagt ggtgtttaac
     4801 gaaattacta aaaacgcgat ccgccaggcg tttaacaaac cgggtgagct gaatattgat
     4861 cgtgttaatg cccagcaggc gcgtcgcttt atggaccgcg tggtggggta tatggtttcg
     4921 ccgctgctat ggaaaaagat cgctcgtggt ctgtctgccg gtcgtgtgca gtcggtggcg
     4981 gttcgcctgg tggtcgagcg tgagcgtgaa attaaagcgt tcgtgccgga agagttctgg
     5041 gaagtcgatg ccagcacgac cacgccatct ggtgaagcgt tggcgttgca ggtgactcat
     5101 cagaacgaca aaccgttccg tccggtcaac aaagaacaaa ctcaggctgc ggtaagtctg
     5161 ctggaaaaag cgcgctacag cgtgctggaa cgtgaagaca aaccgacaac cagtaaacct
     5221 ggcgctcctt ttattacctc tacgctgcaa caagctgcca gcacccgtct tggatttggc
     5281 gtgaaaaaaa ccatgatgat ggcgcagcgt ttgtatgaag caggctatat cacttacatg
     5341 cgtaccgact ccactaacct gagtcaggac gcggtaaata tggttcgcgg ttatatcagc
     5401 gataattttg gtaagaaata tctgccggaa agtccgaatc agtacgccag caaagaaaac
     5461 tcacaggaag cgcacgaagc gattcgtcct tctgacgtca atgtgatggc ggaatcgctg
     5521 aaggatatgg aagcagatgc gcagaaactg taccagttaa tctggcgtca gttcgttgcc
     5581 tgccagatga ccccagcgaa atatgactcc acgacgctga ccgttggtgc gggcgatttc
     5641 cgcctgaaag cacgcggtcg tattttgcgc tttgatggct ggacaaaagt gatgcctgca
     5701 ctgcgtaaag gcgatgaaga tcgtatctta ccggcagtcg ataaaggcga tgctctgacg
     5761 ctcgttgaac tgacaccagc ccagcacttt accaagccgc cagcccgttt cagtgaagca
     5821 tcgctggtta aagaactgga aaaacgcggt atcggtcgtc cgtctaccta tgcgtcgatc
     5881 atttcgacca ttcaggatcg tggctatgtg cgtgtagaaa accgtcgttt ctatgcggaa
     5941 aaaatgggcg aaatcgtcac cgatcgcctg gaagagaatt tccgcgagtt aatgaactac
     6001 gacttcaccg cgcagatgga aaacagcctc gaccaggtgg caaatcacga agcagagtgg
     6061 aaagctgtac tggatcactt cttctcggat ttcactcagc agttagataa agctgaaaaa
     6121 gatccggaag aggggggtat gcgtccgaac cagatggttc tgaccagcat cgactgcccg
     6181 acctgtggtc gcaaaatggg gattcgcaca gcgagcaccg gggtattcct tggctgttct
     6241 ggctatgcgc tgccgccgaa agagcgttgc aaaacaacca ttaacctggt gccggaaaac
     6301 gaagtgctga acgtgctgga aggcgaagac gctgaaacca acgcgctgcg cgcaaaacgt
     6361 cgttgcccga aatgcggcac ggcgatggac agctatctca tcgatccgaa acgtaagttg
     6421 catgtctgtg gtaataaccc aacctgcgac ggttacgaga tcgaagaggg cgaattccgc
     6481 attaaaggtt atgacggccc gatcgttgag tgtgaaaaat gtggttctga aatgcacctg
     6541 aaaatggggc gtttcggtaa atatatggcc tgcaccaacg aagagtgtaa aaacacgcgt
     6601 aagattttac gtaacggcga agtggctcca ccgaaagaag atccggtacc attaccggag
     6661 ctgccgtgcg aaaaatcaga tgcctatttc gtgctgcgtg acggtgctgc cggtgtgttc
     6721 ctggcggcca ataccttccc gaaatcgcgt gaaacgcgtg cgccgctggt ggaagagctg
     6781 tatcgcttcc gcgatcgtct gccggaaaaa ctgcgttatc tggccgatgc gccgcagcag
     6841 gatccggaag gtaataagac tatggttcgc tttagccgta aaaccaaaca tcaatatgtc
     6901 tcttcggaaa aagacggaaa ggcgactggc tggtcagcat tttatgttga tggcaaatgg
     6961 gttgaaggga aaaaataacc tttaattctg tcaggttttt ataaacaaag ggtcgcgaaa
     7021 gcggcccttt tttattgcat attatttttt cttcacacct atacactaag gctataaatg
     7081 atatagtggt tatagttagc acctttttta ttattaaatc gtattagtca cccgccaggt
     7141 gtgacgaaaa aacgatgttc tgatggcgtc taagtggatg gtttaacatg aaattacaac
     7201 aacttcgcta tattgttgag gtggtcaatc ataacctgaa tgtctcatca acagcggaag
     7261 gactttacac atcacaaccc gggatcagta aacaagtcag aatgctggaa gacgagctag
     7321 gcattcaaat tttttcccga agcggcaagc acctgacgca ggtaacgcca gcaggacaag
     7381 aaataattcg tatcgctcgc gaagtcctgt cgaaagttga tgccataaaa tcggttgccg
     7441 gtgagcacac ctggccggat aaaggctcgc tgtatatcgc caccacgcat acccaggcac
     7501 gctacgcgtt accaaacgtc atcaaaggct ttattgagcg ttatccccgc gtttctttgc
     7561 atatgcacca gggctcgccg acacaaattg ctgatgccgt ctctaaaggt aatgcagatt
     7621 tcgctatcgc cacagaagcg ctgcatctgt atgaagattt agtgatgtta ccgtgctacc
     7681 actggaatcg ggctattgta gtcactccgg atcacccgct ggcaggcaaa aaagccatta
     7741 ccattgaaga actggcgcaa tatccgttgg tgacatatac cttcggcttt accggacgtt
     7801 cagaactgga tactgcgttt aaccgcgcag ggttaacgcc gcgtatcgtc ttcacggcaa
     7861 cggatgctga cgtcattaaa acttacgtcc ggttagggct gggggtaggg gtcattgcca
     7921 gcatggcggt ggatccggtc gccgatcccg accttgtgcg cgttgatgct cacgatatct
     7981 tcagccacag tacaaccaaa attggttttc gccgtagtac ttttttgcgc agttatatgt
     8041 atgatttcat tcagcgtttt gcaccgcatt taacgcgtga tgtcgttgat gcggctgtcg
     8101 cattgcgctc caatgaagaa attgaggtca tgtttaaaga tataaaactg ccggaaaaat
     8161 aatttcttgc gttattttcg gcaccttttt tgtagcgaag gtgccgaaat atattttc
//
LOCUS       DAZFKF010000167         8160 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFKF010000167 DAZFKF010000000
VERSION     DAZFKF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.169"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            161..5581
                     /locus_tag="ACHKA1_004882"
     CDS             161..5581
                     /locus_tag="ACHKA1_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
                     /protein_id="HFO5682424.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNGQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGGNEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     ANSNGKVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATILEGGLITAENTIIGGNATGV
                     GTLNVQDQDSVITVRRLYNGYFGNGAVNISNNGLINSKDYSLVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSSEGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGTGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLN
                     VEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMG
                     SSDVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSSDKMFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDIENTTSVNVGEQSIGGLAMNGGTLIFDTDI
                     PAATLAEGYISVDTLVVGVSDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDNHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGV
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTG
                     QMGSTVLFKEGSLTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSVSPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDLTKLGTGILT
                     LNNDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDVNVMSGG
                     TLRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPT
                     DKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVY
                     TLAKGKGNDEKNWYLTSKWDGVTPADTPDPINNPPVVDPEGPSVYRPEAGSYISNIAA
                     ANSLFSHRLHDRLGEPQYTDSRLSQGSASSMWMRHVGGHERFRTGDGQLNTQANRYVL
                     QLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQ
                     NDSNKTGAYVDCWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEG
                     TLNTWYVQPQAQITWMGVKDSDHNRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDG
                     KQREFQPYIEANWINNSKVYAVKMNGQTVSRDGARNLGEVRTGVEAKVNNNLSLWGNV
                     GVQLGDKGYSDTQGMLGVKYSW"
     gene            5792..6058
                     /gene="hipB"
                     /locus_tag="ACHKA1_004883"
     CDS             5792..6058
                     /gene="hipB"
                     /locus_tag="ACHKA1_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO5682425.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTSLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            6058..7380
                     /gene="hipA"
                     /locus_tag="ACHKA1_004884"
     CDS             6058..7380
                     /gene="hipA"
                     /locus_tag="ACHKA1_004884"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO5682426.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK"
     gene            7651..7920
                     /locus_tag="ACHKA1_004885"
                     /pseudo
     CDS             7651..7920
                     /locus_tag="ACHKA1_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2233 a         1739 c         2212 g         1976 t
ORIGIN      
        1 aatctatcaa ttcgaaagca ttagcaaatt agccaaattg acaccttcgc acctccaact
       61 aacactcaat acagacctct gacaatttaa taagtttcat ttttaatgtc tgacgactca
      121 tctctttcgt ggctgtattg tttgtatatg gagcgtttat atgaatagaa tctatcgcgt
      181 gatatggaat tgcactctac aggtatttca ggcctgctcg gaattaactc gcagggcagg
      241 taaaacatcg acggttaatt tgcgtaaatc ctctggactg acaacgaaat tcagtagatt
      301 gacgctgggt gttttgctgg cactaagcgg ttcagcgtct ggtgcgagtc tggaagttga
      361 taatggtcag attaccaata ttgatactga tgttgcttat gatgcctacc tggttggctg
      421 gtatggcact ggagtgctta atattttggc tggcggtaat gcctccttaa ccactattac
      481 taccagcgtc attggcggta atgaggactc agagggcacc gttaatgttt tgggtggcac
      541 ctggcgattg tatgatagcg gaaataatgc aaggccttta aatgtgggtc aatccggaac
      601 ggggacgctg aatattaaac agaaaggtca cgttgatgga ggctatttaa gactaggttc
      661 ctcgacagga ggcgtcggga cggtcaatgt tgagggagag gactcggttc tgacgaccga
      721 attattcgaa atagggagtt acggtacggg ctcattaaat attacggata agggttacgt
      781 cacgagttca atagtcgcca ttttaggata tcaagcgaac agtaatggta aggttgtcgt
      841 tgaaaagggt ggcgagtggc taataaaaaa taatgattcc tcaattgaat ttcaaattgg
      901 taatcaagga actggggagg cgactattct cgagggtggg ctgattacgg ctgaaaatac
      961 gattatcggt ggcaatgcca ccggtgtcgg aaccctgaat gtgcaggatc aagactctgt
     1021 catcacggta cgcagactct ataatggata tttcggtaat ggcgcagtca atatttccaa
     1081 taatggacta attaatagca aagattattc attggtgggc gttcaggacg gttcccacgg
     1141 tgtcgtcaac gtgaccgata aagggcattg gaatttcctc ggaacaggcg aagctttccg
     1201 ctatatctat atcggtgatg ctggcgacgg tgaacttaat gtctcgagtg aaggcaaagt
     1261 agattcggga attatcactg cggggatgaa agaaacaggc acaggcaaca ttactgttaa
     1321 ggataagaac tccgttatca ctaatctcgg aactaatctt ggttatgacg gccacggcga
     1381 aatgaatatc agtaatgagg ggcttgttgt cagcaacgga ggaagttcac tcggttatgg
     1441 agaaaccggc gtcgggaatg tcagcatcac cacggggggg atgtgggagg tcaataagaa
     1501 tgtctatacc accattggtg ttgcgggtgt cggaaacctc aatattagcg atggcggtaa
     1561 gtttgtatcg caaaatatta cttttttggg cgataaagca agcggtatcg gcacactgaa
     1621 cctgatggat gcgacatcat cgttcgatac tgtgggtatc aatgtcggta attttggtag
     1681 cggtatcgta aatgtcagta atggtgccac ccttaattca acgggctatg gatttatcgg
     1741 aggaaatgcc tccggtaagg gaatagttaa tatttcaacg gatagtctct ggaatttaaa
     1801 gacgtcttct actaacgccc aattgctaca ggtcggtgta ttaggcacgg gtgaactgaa
     1861 tattaccacc ggaggtatag ttaaagcgcg tgatacacag atagctctca atgacaaaag
     1921 taagggcgac gtgagggtgg atgggcagaa ctctcttctt gaaacattca atatgtacgt
     1981 agggacatct ggtacgggta cgttaaccct gacgaatagc ggcacgctga atgtcgaagg
     2041 tggagaagtt tacttaggtg tttttgaacc tgctgtagga acgctaaaca ttggtgcagc
     2101 tcacggtgag gcggcggcag atgccgggta tataaccaac gcgacaaaag tagagtttgg
     2161 ctctggcgaa ggtgtttttg tctttaatca taccaataac agtgatgccg gctaccaggt
     2221 cgatatgctg attacaggtg acgataaaga cggaaaagtg atccatgatg caggccatac
     2281 ggtgttcaat gcagggaata cttatagcgg taaaacgctg gtcaatgacg gcctcctgac
     2341 cattgcgtct catacggcag atggggtaac gggcatgggt tcgagtgatg taaccattgc
     2401 aagccccggt acgctcgaca ttctcgcatc aacgaacagt gcaggagatt acacgctgac
     2461 caatgcgctc aaaggcgatg gcttgatgcg agttcagctg tcatcctccg acaagatgtt
     2521 tggctttacc catgcaacag ggactgaatt cgccggtgtt gcccaactga aagacagtac
     2581 cttcactctg gaacgcgaca acaccgctgc gcttactcac gcgatgttgc agtctgacat
     2641 tgaaaatacc acatcggtaa acgtgggaga gcaatccatt ggtggactgg ccatgaatgg
     2701 cggtacgctc attttcgata cggatattcc tgctgcgacg cttgcagagg gatatatcag
     2761 cgtcgatacg ctggttgtcg gcgtgagtga ctacacctgg aaaggccgta actatcaggt
     2821 aaacgggacg ggcgacgtgc ttatcgacgt gcctaaaccg tggaatgatc ctatggcgaa
     2881 taaccctctg acgacgctca atttgctgga acacgatgat aaccatgtcg gcgttcaact
     2941 ggtgaaggcg caaacggtta ttgggtcggg tggctcatta acgttacgtg atttacaggg
     3001 cgacgaggtg gaagcggaca aaacgttaca cattgcgcaa aacggaacgg tggtcgccga
     3061 gggtgattat ggagtccgcc tcacgaccgc accaggtgat ggtttgtacg ttaactatgg
     3121 gctgaaagcg ctgaacatcc atggagggca aaagctgacg ttagccgaac atggcggagc
     3181 ctatggcgca acggccgata tgtcggcaaa aatcggtggt gaaggggatc tggcaatcaa
     3241 tacggtgcga caggtttcgc tttccaacgg tcagaacgac tatcaggggg caacctacgt
     3301 tcagatgggg acattacgta ccgatgcgga tggcgcgctg ggcaacaccc gggaactgaa
     3361 catcagcaac gcagccatcg tcgatcttaa tggatcgacg cagacggtag agacattcac
     3421 cgggcagatg ggttcgactg ttttgttcaa agaagggtcg ctgacggtaa ataaaggtgg
     3481 gatcagtcag ggtgaactga caggtggcgg aaacctgaat gttacagggg gaacgctggc
     3541 tattgagggg cttaatgcac gctacaatgc gttaaccagc gttagcccaa atgcggaagt
     3601 cagcctcgat aatacgcagg ggttaggcag aggaaatatt gccaatgacg gtctgttaac
     3661 gctaaaaaac gtgactggcg aactgcgtaa tagcataagc gggaagggta tcgtgagcgc
     3721 aaccgccagg acagatgtag agctggatgg cgataatagc cgctttgtgg ggcaattcaa
     3781 cattgataca ggcagcgcgc tcagcgtcaa cgagcagaaa aacctgggtg atgcttccgt
     3841 tatcaataat ggcctgctca ccatctccac tgagcgtagc tgggcgatga cgcacagtat
     3901 cagcggtagc ggtgatttga caaaactggg taccgggatc ctgactctta acaacgattc
     3961 cgcggcgtat cagggtacga cggatatcgt gggtggtgaa attgctttcg gttccgactc
     4021 tgccattaat atggcaagtc agcacattaa tatccataac agcggtgtga tgtcgggaaa
     4081 tgtcaccact gcaggtgatg tgaacgttat gtctgggggg acactgcgtg tcgctaaaac
     4141 cacaatcggc ggcaacctgg agaatggcgg cacggttcaa atgaacagtg aaggaggaaa
     4201 accggggaat gtactgaccg ttaacggcaa ctataccgga aacaatggcc tgatgacgtt
     4261 caacgcgacg ctgggcggcg ataattcacc caccgataag atgaacgtga aaggcgatac
     4321 ccaagggaac actcgcgttc gggttgataa cattggcggc gtcggtgcgc aaacggtcaa
     4381 cggtattgaa ctcattgagg ttggcggtaa ttctgcaggt aacttcgcgt tgaccaccgg
     4441 aactgtcgaa gctggggctt acgtctacac gctggctaaa gggaagggga atgacgagaa
     4501 aaactggtat ctgaccagta aatgggacgg cgtaacgcca gcggatacac ccgatcccat
     4561 caataatccc cctgttgtgg atccggaagg cccatcagtt tatcgcccgg aggccggaag
     4621 ctatatcagc aacattgccg cagccaactc gctgtttagc catcgcttac acgaccgtct
     4681 gggtgagccg cagtatacag attcacggct ttctcagggt tcggcaagca gtatgtggat
     4741 gcgtcatgtc gggggacacg aacgtttcag gaccggtgac ggccagctaa atactcaggc
     4801 taaccgctat gtattgcagc taggcggcga tttggcgcag tggagtagca acgcgcagga
     4861 tcgctggcat cttggcgtga tggcaggcta cgccaatcag cacagtaata ctcagagtaa
     4921 tcgtgtgggt tataaatcgg atgggcgcat cagcggttac agcgctgggc tgtacgcgac
     4981 ctggtatcag aacgattcga ataagaccgg cgcttatgtt gactgctggg cgctgtataa
     5041 ctggtttgat aacagcgtca gttccgataa ccgttctgct gacgactatg attctcgcgg
     5101 tgttacggcc tctgttgagg gtgggtatac ctttgaagcg ggaacattta gcggcagcga
     5161 agggacgctg aatacctggt acgtccagcc acaggcgcaa atcacctgga tgggtgtgaa
     5221 agattctgac cataaccgga aagacggaac acgcattgaa acggaaggcg acggaaacgt
     5281 gcaaacgcga cttggggtga aaacctacct gaatagccat caccagcgtg acgatggtaa
     5341 acagcgtgag ttccagcctt acattgaagc gaactggatc aacaatagca aagtctacgc
     5401 cgtgaagatg aatggtcaaa ccgtaagccg tgatggtgcg cgtaatctcg gtgaagtacg
     5461 taccggggtt gaggcgaaag taaataacaa ccttagcctg tgggggaatg tcggtgtgca
     5521 actaggtgat aaaggctata gcgatactca gggcatgctg ggagtgaaat atagctggta
     5581 aacagtataa gccgcatgtc gagatggcat gcggcttaat attgccaact tcaaaaggct
     5641 tttaaagcct cccttttatc cgcgatcgcg gatatcgcag cgtttatccc gtacagcgga
     5701 taagatgtgt ttccagattg actttatcct cactaaagga taaaactgat aatatcccct
     5761 taagcggata aatttgctgt ggacgtatga catgatgagc tttcagaaga tctatagccc
     5821 aacgcaatta gcgaatgcaa tgaaactggt tcgccagcaa aatggctgga cgcagagcga
     5881 gctggcgaaa aaaatcggca ttaagcaggc gacgatttcc aatttcgaaa acaaccctga
     5941 caatacctcg ctcacgacat tttttaagat tttacagtcg cttgaactct caatgacgct
     6001 atgcgacacg aaaaatgcct cgccagaatc aacagaacag caagatctgg agtggtaatg
     6061 cccaaactag tcacttggat gaacaaccag cgggtaggcg agttaacgaa gttagccaac
     6121 ggcgcgcaca cctttaagta tgcaccggag tggttagcca gccgttatgc cagaccgttg
     6181 tcactttcgc tgccattgca gagggggaat atcacctctg atgccgtatt taacttcttc
     6241 gataacttgt tacccgatag cccgattgta cgtgaccgga tcgttaaacg ttatcatgct
     6301 aaatccagac aaccgtttga tttattgtca gaaatagggc gagacagcgt tggtgccgta
     6361 acgttattac ccgaaaatga aaccataacg cgcccgataa tggcatggga aaagcttact
     6421 gaagccagac ttgaagaagt attaacggct tataaagcag atatcccgct aggcatgatt
     6481 agagaagaaa atgactttcg catctcggtt gctggcgcac aggagaagac agcactgctc
     6541 agaataggca atgactggtg cattccgaaa ggaataacgc cgacgacgca catcattaaa
     6601 ttaccgattg gggaaatcag gcagcccaat gcgacgctcg atctcagcca aagcgttgat
     6661 aatgagtatt actgtctgct gctggcgaaa gaacttgggt tgaatgttcc ggacgcagaa
     6721 atcattaaag cgggaaatgt gcgcgcgtta gcggtcgaac gttttgacag gcgttggaat
     6781 gctgagcgaa cggttttact tcgcttgcca caggaggata tgtgtcagac attcggttta
     6841 ccttcatcgg tgaaatatga atcagatgga ggcccaggca tcgcgcggat catggctttt
     6901 ttgatggggt ccagcgaggc gctgaaagat cgctatgatt ttatgaaatt ccaggtcttt
     6961 cagtggttga ttggcgcaac ggatggccat gcaaaaaact tctccgtatt tattcaggct
     7021 ggcggcagtt atcgactcac gccattttac gacatcattt cagcatttcc ggtattgggt
     7081 ggcgcgggaa tacacatcag cgatctcaaa ctggcaatgg ggcttaacgc atccaaaggc
     7141 aaaaaaacgg caatcgataa aatttatccg cgacattttt tggcgacagc aaaggtgctg
     7201 agattcccgg aagtgcagat gcatgaaatc ctgagtgact ttgccagaat gattccggca
     7261 gcactggata acgtgaagac ttcattaccg acagattttc cagagaacgt ggtgacggca
     7321 gttgaaacca atgtgttgag gttgcacggt cggttaagcc gagaatacgg tattaagtaa
     7381 gagtaagata tggggtttgg tcgttgttaa tgagcaggac aatcatgacc gccaacgact
     7441 agattcttcc ttggtttatg ttggtgcgca taatgtatat tatgttaaat cgtgtctgag
     7501 tgatacgaag tgattttccg gtagtcgtta ttcgttcagg gttccagaac ttgagtctct
     7561 cctctttcat aattggcctg tgcatgccgg atataggctg gctggtatgc agatttgccg
     7621 tttgccgcat cttcccagcg ttgaagactc aagctggtaa accgcgtgca aatgagcacg
     7681 ccctctttcc atcaacactt caggcagctt gcgggaatga gtccactccg gtatcagaaa
     7741 tggctacgtc tcaatgaagt caggcgaacg atgctgaatg aacattacga tgtcaccaca
     7801 gcagcctatg ctgtcggtta cgaaagctta tcccatttca gccgggaata tttgcggatg
     7861 tttggagagt cacctaagag agatattacc aggttaagaa agtctgtcgg tcagctttaa
     7921 taaaccctgc acttatctgt ttagagcaat tcggtgttag ttacaccagg caaacattaa
     7981 ctatagctaa tgatttatag ccatataaac cattagtagc ccttgacctt tcctgacact
     8041 tcccttgttc gcaataatat tctgtttagt cacggtttat aaataacatg cataatgaaa
     8101 tcgtcatgtt atttagcgac ataaaatgtt tttgcaaata ctctcttcaa caagtgcagc
//
LOCUS       DAZFKF010000168         8034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFKF010000168 DAZFKF010000000
VERSION     DAZFKF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.170"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..700
                     /locus_tag="ACHKA1_004886"
     CDS             <1..700
                     /locus_tag="ACHKA1_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO5682427.1"
                     /translation="DRFSTIFGDKYILTLLNSMRNMEGNKDSRINKNVVVLAGNHEIN
                     FNGNYSARLANHKLSSGDTYNLIKTLDVCNYDSETKVLTSHHGIIRDEENKCYCLGAL
                     QVPFNQMKNPTDPEELAYIFNKKHKQHMDDHLFHLIRSNTMAPTPVYADYLNNTTDFR
                     PDPEDIFKCGQTLECGDPRKNIRQKYGHHGLGVNQNQQFDNGVMGLNSRMAVFDENKK
                     IIGSSSGLSCFQPR"
     gene            961..2577
                     /locus_tag="ACHKA1_004887"
     CDS             961..2577
                     /locus_tag="ACHKA1_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO5682428.1"
                     /translation="MKIGTVAGTNDSITTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRR
                     NNSYQFIVTINSKNYGCPLDDTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVSDIKFEKIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVIISILNRRDIILESLQPY
                     LVINKGAVTPCTFLGDQTGDRFSNICGDQFIIDLLKCIMSINDNVHVLAGNHETNCNG
                     NYMQNFTRMKPLDEDTYAGIKDYPVCFYDSKYKIMANHHGITFDDQRKRYIIGPITVS
                     IDEMTNALDPVELAAIINKKHHTIINSKKFKTSRAISCRSFNRYFSVSTDYRPKLEAL
                     LACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHTGRMFSMHNCQINPGAGP
                     EITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2767..2880
                     /gene="yhiY"
                     /locus_tag="ACHKA1_004888"
     CDS             2767..2880
                     /gene="yhiY"
                     /locus_tag="ACHKA1_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO5682429.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2943..4010
                     /gene="yhiI"
                     /locus_tag="ACHKA1_004889"
     CDS             2943..4010
                     /gene="yhiI"
                     /locus_tag="ACHKA1_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5682430.1"
                     /translation="MDKSKRHLAWWGVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            4007..6742
                     /gene="rbbA"
                     /locus_tag="ACHKA1_004890"
     CDS             4007..6742
                     /gene="rbbA"
                     /locus_tag="ACHKA1_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO5682431.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDATHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6742..7866
                     /gene="yhhJ"
                     /locus_tag="ACHKA1_004891"
     CDS             6742..7866
                     /gene="yhhJ"
                     /locus_tag="ACHKA1_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5682432.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
BASE COUNT         2052 a         1854 c         2092 g         2036 t
ORIGIN      
        1 cgatagattc agtactattt ttggtgataa atatatcctt actttgctaa attctatgcg
       61 aaatatggag ggtaataaag atagtcgtat aaataaaaat gtcgtcgttc tggctggaaa
      121 tcatgaaatc aattttaatg gtaactatag tgcacgacta gcaaatcata aactctcttc
      181 cggtgataca tataacttaa ttaaaacgct cgatgtttgt aattatgact cagaaacgaa
      241 agttttaacc agtcatcacg gaataataag ggatgaagaa aataaatgtt attgtctcgg
      301 tgctttgcag gttccattta atcagatgaa aaatcctact gatcctgagg aattagcata
      361 tatatttaat aaaaaacata aacaacatat ggatgatcat cttttccatc taattagaag
      421 caatactatg gcacccacac ctgtatatgc tgattattta aataacacta ctgatttcag
      481 accagatcct gaagatatct tcaaatgtgg tcagacgctc gaatgtggcg acccccgcaa
      541 aaacattaga caaaaatatg gccatcatgg gttaggggta aatcagaatc aacaatttga
      601 taatggtgtt atggggctaa attccaggat ggcagtgttt gatgaaaata aaaaaataat
      661 aggttcttcc tcagggctta gttgttttca acctcgttaa aactccatga gtgctgtggt
      721 atgtttttta atcctgccag tattccatag gttttcttaa tcgattgtag aggtatgtac
      781 tgatgacgcc tctacaatct ttagggactt tctctactat gagataagaa atttcatatt
      841 atgctttaag gctgaatgat ttgtgcaaaa tataattatt tttccatcga acatgaggtg
      901 tatgtcataa gattaaatga taactatgta ttcctcgcgg tgaattatat gaggtaaaag
      961 atgaaaattg gaactgtggc aggtactaac gacagtataa cgacaatcgc aacaaatgat
     1021 atggtgcaag agcatgttac caacttcact aaagaactgt ttgggtatat tgcaaatggt
     1081 ataggtgatg atatatcaag tattgccagg acaatgctcg gtgaggttgt ggaaaaaata
     1141 gatgattggc aaatagaacg gttccaacaa tctattcaag atgacaaaat atcatttacc
     1201 atacaaacgg atcattcaga aaaatatagt atgctatcgg gcatgcgtgc ccatattcta
     1261 cgtaggaata atagttatca gtttattgtg acaattaata gtaaaaatta cggttgccct
     1321 cttgatgata ccgatattaa ttggtgcagc attgtttatt tacttaataa tatgacagta
     1381 aatgataatg ctaatgatgt tgcagttact gagtcgtaca aacccatctg gaactggaaa
     1441 atatcgcaat acaatgtatc tgatattaaa ttcgaaaaaa ttataaaacc ccagttcgct
     1501 gacagaacct atttctccaa ttgttcacct gttgatccca caagtaccag accaacctat
     1561 tttggtgata ccgatggctc agttggtgca gttttgtacg cgctttttgc aacgggacat
     1621 ttgggaatca tggctgaagg agaaaatttc cttagccagt tgcttaatat agaagatgaa
     1681 gttctcaatg tattgctaag ggagaacttt aatgagcaac ttgatactaa tgtaataatc
     1741 agtatcctga acagaagaga cattatttta gaaagtcttc agccttattt agttattaat
     1801 aaaggcgcag tgacgccatg tacgtttttg ggtgaccaaa ccggcgatcg atttagtaat
     1861 atctgtggcg atcaatttat catcgatttg ttaaaatgca taatgagcat caatgataac
     1921 gttcatgtat tagctggaaa tcatgaaacg aattgcaatg gtaactatat gcagaacttc
     1981 acgcgtatga aaccacttga tgaggatacc tacgctggga taaaagatta tcctgtatgt
     2041 ttctatgact ccaaatacaa aataatggca aatcatcatg ggataacttt tgatgatcag
     2101 cgaaaacgtt acattatagg accgataacg gtctctattg atgaaatgac caatgctctc
     2161 gatccggtcg aattagcggc aattattaat aaaaagcatc atactataat taatagtaaa
     2221 aagtttaaaa ctagccgggc tatctcttgt cgatccttta atcgttattt ttctgtttcg
     2281 acagattaca gacctaaact tgaagcacta ttagcatgct cacaaatgtt aggtataaat
     2341 caagttgttg cacataacgg taatggtgga agagaacgca taggtgaaac ggggacagtt
     2401 ttaggtctta acgcacgtga cagtaaacat acgggaagaa tgttcagcat gcataattgc
     2461 caaattaacc ctggtgcggg gcctgagata accactccct ggaagtctta ccaacatgaa
     2521 aaaaacagaa atggacttat gcctttgatt agaaggcgca caatgttaca gctctgaacc
     2581 atagacaaca tgaatattaa tgttactatc tggttgagac tgttgataaa acgtaataag
     2641 gaatgctctg ttcctgcaac tgaacaggcg tgaaacgcta tccaacagga tggataccgt
     2701 tttgtcagcc agttttatag atcacactta tgagtatgcg tgtttcattt acggccagga
     2761 atgctgatga tgacaacatt actacccgtt ttcactaagc cttccccgtt ggcgctcaat
     2821 gctctgcgcg ctggtcgtat ttgtcgtttc cttcttatcc cggatggaag aatccgctga
     2881 acgtcgttcg gcggcagttg taacacgcgt cgtttgctta cagggattat ggcgggtcga
     2941 ttatggataa gagtaagcgc catctggcgt ggtggggtgt cgggttactg gcggtggctg
     3001 ctatcgtggc gtggtggctg ttgcgcccgg caggtgtgcc ggaaggcttt gccgtcagca
     3061 atgggcgcat tgaagcgacg gaagtggata tcgccagcaa aattgccggg cgtatcgaca
     3121 ccattctggt gaaagaaggc cagtttgttc gcgaaggcga agtgctggcg aagatggata
     3181 ctcgcgtgtt gcaggagcag cgactggaag ccatcgcgca aatcaaagag gcacaaagcg
     3241 ccgttgccgc cgcgcaggct ttgctggagc aacgacaaag cgaaactcgc gccgcacagt
     3301 cgctggttaa tcaacgccag gccgaactgg actccgttgc caaacgtcat acgcgttccc
     3361 gctcgctcgc ccaacgaggg gctatttctg cgcaacagct ggatgacgat cgcgccgccg
     3421 ctgagagcgc ccgagctgcg ctggaatcag caaaagccca ggtttcggct tctaaagccg
     3481 ctatagaagc ggcacgcacc aatatcattc aggcgcaaac ccgcgtcgaa gccgcacaag
     3541 ccactgaacg gcgcattgcc gcagatatcg atgacagcga actgaaagcc ccgcgtgacg
     3601 gacgcgtgca gtatcgggtt gccgagccag gggaagtgct ggcggcaggc ggtcgggtgc
     3661 tgaatatggt cgatctcagc gacgtctata tgaccttctt cctgccaacc gaacaggcgg
     3721 gcacgctgaa acttggcggt gaagcccggc tgatcctcga tgccgcgcca gatctgcgta
     3781 ttcctgcaac catcagtttt gtcgccagtg tcgcccagtt cacgccaaaa accgtcgaaa
     3841 ccagcgatga acggctgaaa ctgatgttcc gcgtcaaagc gcgtatccca ccggaattac
     3901 tccagcagca tctggaatat gtcaaaacgg gcttgccggg cgtagcgtgg gtgcgggtga
     3961 atgaagaact tccgtggcct gacgacctcg tggtgaggtt gccgcaatga cgcatctgga
     4021 actggttccc gtcccgcctg tcgcgcaact ggcgggcgtg agccagcatt atggaaaaac
     4081 cgttgcgctg aacaatatca cactcgatat tccggcccgc tgtatggtcg ggctgattgg
     4141 tccggatggc gtcgggaagt cgagtttatt gtcattgatt tccggtgctc gtgtcattga
     4201 gcagggcaat gtgatggtgc tgggcggcga tatgcgcgac ccgaagcatc gccgtgacgt
     4261 ttgcccgcgc atcgcctgga tgccgcaggg gctgggcaaa aatctctacc acaccttatc
     4321 ggtgtatgaa aacgtcgatt ttttcgctcg cctgttcggt cacgacaaag cggagcggga
     4381 agtccgaatt aatgagctgc tgaccagcac cgggttagca ccgtttcgcg atcgtccggc
     4441 agggaaactc tccggcggga tgaagcaaaa actggggctg tgctgtgcgt taatccacga
     4501 cccggaactg ttgattcttg atgagccaac aacgggggtt gacccgctct cccgcgccca
     4561 gttctgggat ctgatcgaca gtattcgcca gcggcagagc aatatgagcg tgctggtcgc
     4621 caccgcctac atggaagagg ccgaacgctt cgactggctg gtagcgatga atgccggaga
     4681 agtgctggca accggcagcg ccgaagagct gcggcagcaa acgcaaagtg ccacgctgga
     4741 agaggcgttt ataaatctgt taccgcaagc gcaacgccag gcgcatcagg cggtagtgat
     4801 cccaccatat caacctgaaa acgcagagat tgccatcgaa gcgcgcgatc tgaccatgcg
     4861 ttttggttcc ttcgttgccg ttgatcacgt taatttccgc attccacgcg gggagatttt
     4921 tggttttctt ggttcgaacg gctgcggtaa atccaccacc atgaaaatgc tcaccggact
     4981 gctgcccgcc agcgaaggtg aggcgtggct gtttgggcaa ccggttgatc caaaagatat
     5041 cgatacccgc cgtcgggtgg gctatatgtc gcaggcgttt tcgctctata acgaactcac
     5101 cgtgcggcaa aacctggagt tacatgcccg tttgtttcac atcccggaag cggaaattcc
     5161 cgcgcgagtg gctgaaatga gcgagcgttt taagctcaac gacgttgaag atgttctgcc
     5221 ggagtcattg ccgctcggca ttcgccagcg gctttcgctg gcggtggcgg tgattcatcg
     5281 cccggaaatg ttaatcctcg atgagcctac ttctggcgtc gatccggtgg cgagggatat
     5341 gttctggcag ttgatggtcg atctctcgcg ccaggacaaa gtgaccattt tcatctccac
     5401 ccactttatg aacgaagcgg aacgttgcga ccgtatctca ctgatgcacg ccggaaaagt
     5461 gctcgccagc ggtacaccgc aggaactggt tgagaaacgc ggagccgcca gtctggaaga
     5521 ggcatttatc gcctatttgc aggaagcggc aggacagagc aacgaagccg aagcgcctcc
     5581 cgtgatacac gatgctacac acgcgccgcg ccagggattt agcctgcgcc gtctgtttag
     5641 ctacagccgg cgcgaagcac tggaattgcg ccgcgatccg gtccgttcga cgctggcgct
     5701 gatgggaacg gtgatcctga tgctgataat gggttacggc atcagtatgg atgtggaaaa
     5761 cctgcgcttt gcggtgctcg accgcgacca gaccgtcagt agtcaggcgt ggacgctcaa
     5821 cctctccggt tcccgttact ttatcgaaca gccgccgctt accagttatg acgagcttga
     5881 tcgtcggatg cgtgcgggcg atatcacggt ggcaattgag atccctccaa atttcgggcg
     5941 tgatatcgcg cgtggtacgc ctgtggaact cggcgtctgg atcgacggag cgatgcccag
     6001 tcgtgccgaa acggtaaaag gttacgtgca ggctatgcac cagagctggc tacaggatgt
     6061 ggcgagccga caatccacac cctccagcca aagcgggctg atgaacattg agacgcgcta
     6121 tcgctataac ccggacgtaa aaagcctgcc agcgattgtt ccggcggtga tcccgcttct
     6181 gctgatgatg atcccgtcaa tgctaagcgc ccttagtgtg gtgcgggaaa aagaactagg
     6241 gtcgattatc aacctttacg tgactcccac cacccgtagc gaatttttgc ttggcaaaca
     6301 gctaccgtac atcgcgctgg ggatgctgaa ctttttcctg ctctgcggtc tgtcggtgtt
     6361 tgtgtttggc gtaccgcata aaggcagttt cctgacgctc accctggcgg cgctgctgta
     6421 tatcatcatc gccaccggaa tggggctgct gatctccacc tttatgaaaa gccagattgc
     6481 cgccattttc ggtacggcga ttatcacgtt aatcccggcg acacagtttt ccgggatgat
     6541 cgatccggta gcttcgctgg aaggtccagg acgttggatc ggcgaggttt acccgaccag
     6601 tcattttctg actatcgccc gcggaacgtt ctcgaaagcg ttggatctga ctgatttatg
     6661 gcaacttttt atcccgttgc tgatagccat accgctggtg atgggcttaa gcatcctgct
     6721 gctgaaaaaa caggagggat gatgcgccat ttacgcaata tttttaatct gggtatcaaa
     6781 gagttgcgca gtctactcgg tgataaagcg atgctgacat tgattgtctt ctcgtttacg
     6841 gtgtcggtgt attcgtcagc gaccgttacg ccaggatcgt tgaacctggc accgatcgcc
     6901 attgccgata tggatcaatc gcagttatcg aatcggatcg ttaacagctt ctatcgcccg
     6961 tggtttttgc caccggagat gatcaccgcc gatgagatgg atgccggact ggacgccgga
     7021 cgctatacct tcgcgataaa tattccgcct aattttcagc gtgatgtcct cgctggacgc
     7081 cagccagata ttcaggtgaa cgtcgatgcc acgcgcatga gccaggcatt taccggcaac
     7141 gggtatatcc agaatattat caacggtgaa gtgaacagct ttgtcgcgcg ctaccgtgat
     7201 aatagcgagc cgttggtatc gctggaaacc cggatgcgct ttaacccgaa cctcgatccc
     7261 gcgtggtttg gcggggtgat ggcgatcatc aacaacatta ctatgctggc gattgtattg
     7321 accggatcgg cgctgatccg cgagcgtgaa cacggcacgg tggaacactt actggtgatg
     7381 ccgataacgc cgtttgagat catgatggcg aaggtctggt cgatggggct agtggtgctg
     7441 gtggtatcgg gattatcgct ggtgctgatg gtgaaaggcg tgctgggcgt accgattgaa
     7501 ggctcgatcc cgctgtttat gctgggcgtg gcgctcagtc tgtttgccac cacgtcaatc
     7561 ggcattttta tggggacgat agcgcgttca atgccgcaac tggggctgct ggtgattctg
     7621 gtgctgctgc cgctgcaaat gctttccggt ggatccacgc cgcgcgaaag tatgccgcag
     7681 atggtgcagg acattatgct gaccatgccg acgacacact ttgttagcct cgcgcaggcc
     7741 attctctacc ggggtgccgg attcgaaatc gtctggccgc agttcctgac gctgatggca
     7801 attggcggcg cgtttttcac cattgcgctg ctgcgattca ggaagacgat tgggacaatg
     7861 gcgtaaatac aattgcccga tgcgtaagct tatagggact acgcatcggt gcaatttatt
     7921 gaattttagt tgtttttagg ccggataagg cgtttatgaa atgaccccca aaacctggac
     7981 agtttcagtt aaccggcttt ttgatactca gtccggtatt cagggcaaga ttac
//
LOCUS       DAZFKF010000169         7946 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFKF010000169 DAZFKF010000000
VERSION     DAZFKF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.171"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            316..1041
                     /locus_tag="ACHKA1_004892"
     CDS             316..1041
                     /locus_tag="ACHKA1_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5682433.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            999..1676
                     /gene="bglJ"
                     /locus_tag="ACHKA1_004893"
     CDS             999..1676
                     /gene="bglJ"
                     /locus_tag="ACHKA1_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO5682434.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1714..2502)
                     /gene="fhuF"
                     /locus_tag="ACHKA1_004894"
     CDS             complement(1714..2502)
                     /gene="fhuF"
                     /locus_tag="ACHKA1_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO5682435.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDPTLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNVLSSLLAVYSDHIYRNQPMMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATPHSPQHRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2643..2879
                     /locus_tag="ACHKA1_004895"
     CDS             2643..2879
                     /locus_tag="ACHKA1_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO5682436.1"
                     /translation="MLQRMLGSGWGVLLPGLLIAGLTYADLSPDKWRIVILMGLLLTP
                     LMLYHKQLRHYVLLPSCLALSAGIMLMLMNLNQG"
     gene            complement(2918..3004)
                     /locus_tag="ACHKA1_004896"
     tRNA            complement(2918..3004)
                     /locus_tag="ACHKA1_004896"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Leu,seq:cag)
     gene            complement(3272..4303)
                     /gene="rsmC"
                     /locus_tag="ACHKA1_004897"
     CDS             complement(3272..4303)
                     /gene="rsmC"
                     /locus_tag="ACHKA1_004897"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO5682437.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGIEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4406..4819
                     /gene="holD"
                     /locus_tag="ACHKA1_004898"
     CDS             4406..4819
                     /gene="holD"
                     /locus_tag="ACHKA1_004898"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO5682438.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4788..5234
                     /gene="rimI"
                     /locus_tag="ACHKA1_004899"
     CDS             4788..5234
                     /gene="rimI"
                     /locus_tag="ACHKA1_004899"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO5682439.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5249..5926
                     /gene="yjjG"
                     /locus_tag="ACHKA1_004900"
     CDS             5249..5926
                     /gene="yjjG"
                     /locus_tag="ACHKA1_004900"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO5682440.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            6312..7535
                     /locus_tag="ACHKA1_004901"
     CDS             6312..7535
                     /locus_tag="ACHKA1_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5682441.1"
                     /translation="MALSDVWLRSVVGKERDKVLVKSDRDGLSVRVSPKGRVVFQYRY
                     QWAGKGERLDIGTYPATGLKEAREEVIRLRGELESNRNPRLVKQAEKRKVTEAMTVES
                     VIRAWYEAYCVKNKKGSEQILRSFELHLFSKIGNIPHDAATLHDWLEVLEPLSTKTPA
                     IADRLLINAKQAHVWAYKRKLIETRPLSDITGKDMDIRKGQKKRFLTHDEIKILYAAI
                     DGSRMVPKYRAFIKLLLHFGCRSSELITARVDDFDFINKVWTVPPERHKTGEITGEPL
                     KRPIIEPVEELIKYAISMNNGSDMLFTKEGSREPVGRTSLQSLPYNLMQYAWRCLGYQ
                     FPHWSLHDLRRTARTNFSDLTAPHIAEIMLGHKLPGVWQVYDKSDYLEEQRKAYLAWW
                     KRVESIVNCTISDSN"
BASE COUNT         2098 a         1820 c         1982 g         2046 t
ORIGIN      
        1 tgtgattccg caatctgctg gaagggatat cacttaatta taaattaaat aaataaaatt
       61 tctgtttttc attaagtatt tctaacaact taatgtagat attttaaatg tctccaggct
      121 atttcaccag gttatcattt gatgctaacc ttctgacaca tgcagtggag ttgttgtgca
      181 gcaggagtat gctgatatga aagtgaatgc taaggataat ttattcgctt aatctattaa
      241 tttgctggaa acatttaagg aatgaatgtc ctgaataaac catcataatt tttagacagt
      301 ggatgtggag gaaatatgtt gccaggatgc tgcaaaaatg gaattgttat cagtaaaata
      361 cctgttatgc aagcagggtt aaaagaggtc atgaggactc acttccctga atatgaaata
      421 atatccagcg cctctgcgga ggaccttacc ttattacaat tacgtcgttc cggattagtc
      481 attgctgatt tagccggtga aagtgaagat ccacgttctg tttgtgaaca ttattattct
      541 ttaatctcac aatatcggga aattcactgg gttttcatgg tgtcacgctc ctggtattcc
      601 caggcagtag aactgctcat gtgccctacg gcgacgttat tgtctgatgt tgaacccatt
      661 gagaatctgg tcaagaccgt acgttccggc aatacgcacg cagagcgtat cagcgccatg
      721 ctgacctccc cggcaatgac tgaaactcat gattttagct atcgctccgt cattctcact
      781 ctttcagagc gcaaggtact gcggctatta ggtaaaggat ggggcatcaa ccagatagct
      841 tcattgctta agaaaagtaa taaaactatc agcgcccaaa aaaacagtgc gatgcgtcga
      901 ctggcaattc acagcaacgc tgaaatgtat gcatggataa atagcgcgca gggtgcaaga
      961 gaacttaact tgccttctgt ttatggagat gccgcagaat ggaacacagc cgaattaaga
     1021 agagaaatgt cgcactcata gaaaaatgcg tcatgagtag tatcggtatt gagagtttat
     1081 tcagaaagtt tgcgggcaac ccttataagc tccataccta taccagtcag gagtcatttc
     1141 aggatgccat gtcgcggatc tcgtttgcgg cggtcatttt ttctttttct gccatgagaa
     1201 gtgagcgcag agagggatta tcttgcctga ctgaactggc gattaagttt ccgcgtaccc
     1261 ggcgtttagt tattgcggat gatgatattg aagctcggct gattggttca ttgtcgccat
     1321 caccgctgga cggtgtatta agtaaagcgt caacgctgga gatttttcat caggaactct
     1381 ttttgtcatt aaatggtgta cgtcaggcga ccgaccgact gaacaatcag tggtacatta
     1441 accaaagccg gacgttaagc ccgacggaga gagaaatatt gcgctttatg tcgcgtggct
     1501 actcaatgac acaaattgcc gagcagctta aacgcaatat taaaacgatc cgtgcacata
     1561 aatttaatgt gatgtcgaaa ctgggcgtca gttctgacgc agggttgttg gaggccgcag
     1621 atattctgtt atgtatgcgg cattgcgaaa caagtaatgt gttgcatcca tattaatccg
     1681 catgatgccg ggtttacttc cccggcagtg ctttcatttc agcgtacaat cgccacattg
     1741 ctgcacatcc ggtaagcgat aacgctggca gcaagtgcgg cgcaccagca ggccgtcgcg
     1801 tagcaccacg gtacgccaga gtggattatc ttcaccggta gtaagcgttt tctcaaaaaa
     1861 gagggcatgg cgcagcgatt caaccgtcgc ctcgccgagc agttgcttca tctcagtaag
     1921 ataccagttg atcaaataac cggtattact ccagataagt ttgccgttga tctctccggt
     1981 cgcttctagt gcctgcacaa ccggaaccag cgcctggctg attaacgttt ccattcgatg
     2041 ctgcggcgaa tgtggtgttg cgtttttatc ttcacataca tcgacccaga aacaggcggc
     2101 gcgtccggtt tcgtgaaact caacgtggaa atgttccggc gacacatcta atgctttttc
     2161 ctgcgtcagc agcgccagca ttaatggcgg caccatcagg ccgatatacc actgcgccca
     2221 cagtgagatc agcggtttgt tctcgcggat catcatcggt tggttgcgat agatatgatc
     2281 ggaatagacc gccagcagag aacttagcac attcggtgat gaccattgcg ccagcgtcat
     2341 ggcgttaagt ggggcaggtt catccaggcg gataaactcg agcaaatgtt cacgatgttt
     2401 tgcgatcgtc gcccgcacgg cttgcgcaag cgtgggatcc tgcggctgga gatgcgttcg
     2461 ccagataaca tcttcataga gcggtgcgga acgataggcc ataatcggga tagtaatcta
     2521 aatgataatg attgctaatc ctagcgatag gtttacccga tagcaaggga tttctctggc
     2581 ttgcaaatga taaaaattat catatgatat tggttatcat tatcaatgaa agagataaaa
     2641 ccatgttgca acgtatgctg ggcagtggct ggggagtgtt gctgccggga ttgctgattg
     2701 caggactgac gtatgcggat ttatcgccgg ataagtggcg gattgtgatt ctgatgggat
     2761 tactgctgac accgctgatg ttgtatcaca aacagttacg gcattacgtt ttgctaccat
     2821 cgtgcctggc gcttagtgct ggcatcatgc tgatgctaat gaatttgaat cagggatgaa
     2881 aaatcaagga agaaacaaga aaggaagtaa agataattgg tgcgaggggg gggacttgaa
     2941 cccccacgtc cgtaaggaca ctaacacctg aagctagcgc gtctaccaat tccgccacct
     3001 tcgcatgcca tcaattctta aaaagaattg ctaccacgga ggcgcattct agtggttttc
     3061 agcttttcgt caatagttaa ttatcgccgg aggtgtaatt gctggaaaaa tgtccatcag
     3121 gaaactagcg tgcaggtttg gtaggcatgc gggggcagat gccagatgcg acgctggcgc
     3181 gtcttatctg gcctacgaag ggctaacgtg caggttttgt aggtcggata aggcgttcac
     3241 gccgcatccg acacagtatt cggcgggata cttaaccttt cttcgcctgg cgggtcataa
     3301 tggcgcgata caccttgaag cgccctgttt gcgcgataac ttcatggaag ccaaatgtct
     3361 catccagcac gtccggataa ggcaggaagg cgttcgctac aattcgcagc tcgccgccgc
     3421 tattaagatg acgcaccgcg ccgcgaatca gcgtttgcgc cgcatccagg ctggtttgca
     3481 tcccatcgtg gaacggcgga ttggagatga tcatatcaaa acgacctttt acctcggaaa
     3541 agacgttgct ggcaaagact tcaccttcaa taccgttggc ggcaagcgtc gcgcggctgg
     3601 cttcaactgc tggcgcagag acatcgcaca aggtgagacg aattttcggc gaatggcgcg
     3661 caaaggcaac tgaaagcaca cccgcgccac agccgacatc cagcacttta cctttcgtgt
     3721 gcggagttaa cgtcgagagc agcaactggc taccgacatc cagaccgtcg cggctaaaca
     3781 cgccaggcag cgttttgacc gtcaggccat cgacgctgta ttcgccccag aatttatccg
     3841 catcaaatac tggctgtttt tccagacgac caaaatagag tccacagcga cgagcgctgt
     3901 cgactttatt caacggcgca taatctgcca gcatctgctc ggcgctgcgc acgccgctgc
     3961 ggttctcgcc aacgacaaaa atatctgtcc ccactggcag cagagaaagt aaattcatca
     4021 actggaactg ggcttccggt ttgttcttcg gccagtagta aatcagcgta tcgcaatctg
     4081 cgacgtcatc cgccgtggcg accagactga aacgggcgtt atcccccatc tggcggctta
     4141 atacctgcca gtggtggaat tgctgggtat gagcacggct ggccgcggta tctaaacgcg
     4201 cgggcaggtc atcctgtaag tctccggcaa acagaatacg gctttgttcg aaatcatcac
     4261 tgtgacgcag caagacttca cttgccgggg taaatgcaga catggaatgc tcctcaattg
     4321 atactggcgg cgattatagc catatgttgg cgcggtatcg acgaatttgc tatatttgcg
     4381 cccctgacaa caggagcgat tcgctatgac atcccgacga gactggcagt tacagcaact
     4441 gggcattacc cagtggtcgc tgcgtcgccc tggcgcgttg cagggggaga ttgccattgc
     4501 gatcccggca cacgtccgtc tggtgatggt ggcaaacgat cttcccgccc tgactgatcc
     4561 tttagtgagc gatgttctgc gcgcattaac cgtcagcccc gatcaagtgc tgcaactgac
     4621 gccagaaaaa atcgcgatgc tgccgcaagg cagtcgctgc aacagctggc ggttgggtac
     4681 tgacgaaccg ctatcactgg aaggcgctca ggtggcatca ccggcgctca ccgaattacg
     4741 ggcaaaccca acggcacgcg ccgcgttatg gcaacaaatt tgcacatatg aacacgattt
     4801 cttccctcga aacgactgat ttaccagcgg cttaccacat tgaacaacgc gcccacgcct
     4861 ttccgtggag tgaaaaaacc tttgccagca accagggcga gcgttatctc aactttcagt
     4921 taacgcaaaa cggcaaaatg gcggcgtttg cgattacgca agtggtgctg gatgaagcta
     4981 cattgttcaa tattgcggtc gatcctgact atcagcgtca gggattggga agggcgctgc
     5041 tggaacatct gatcgacgaa ctggaaaaac gcggcgtggc gacgctctgg ctggaagtcc
     5101 gtgcctcaaa cgctgccgcc attgccctgt acgaaagttt aggctttaac gaggcgacga
     5161 ttcgccgcaa ttactacccc accacggacg gtcgcgaaga cgccatcatc atggcgttgc
     5221 caatcagtat gtaagacaag gtggaataat gaagtgggac tggattttct ttgatgccga
     5281 tgaaacgttg tttacctttg actcattcac cggcctgcag cggatgtttc ttgattacag
     5341 cgtcaccttt accgctgaag attttcagga ctatcaggcc gttaacaagc cactgtgggt
     5401 ggattatcaa aacggcgcga tcacttcatt acagcttcag cacgggcggt ttgagagctg
     5461 ggccgaacgg ctgaacgtcg agccgggtaa actcaacgaa gcctttatta atgcgatggc
     5521 ggaaatctgt acgccgctgc cgggcgcggt ttctctgctt aacgccattc gtggcaacgc
     5581 caaaatcggc atcatcacca acggctttag cgccttgcag caagtgcgtc tggaacgtac
     5641 gggcctgcgt gattacttcg atttgctggt gatttccgaa gaagttggcg ttgccaaacc
     5701 gaataagaaa attttcgatt atgcgctgga acaggcgggc aatcctgacc gttcacgcgt
     5761 gctgatggtt ggcgacactg ccgagtccga tattctcggt ggcatcaacg ccgggcttgc
     5821 gacctgctgg ctgaatgcgc acaatcgcga gcaaccagaa ggcatcgcgc ccacctggac
     5881 cgtttcttcg ttgcacgaac tggagcagct cctgtgtaaa cactgattgc ctcccccccg
     5941 ttgatgggta aaatagccgc aatttttcgt tttcaacaag cgcggcgcga tgccgcttac
     6001 tcaagaagaa agaattatga cgttgtctcc ttatttgcaa gaggtggcga agcgccgcac
     6061 ttttgccatt atttctcacc ccaataatta agcccaaatt aaagctcttt tactctttca
     6121 aaatcctttc agttaattta ggttgagtta tataactcat ttaaatacat ttggttatgt
     6181 agcttcttta attgaaaact ctttcatgta actttaaatc gatcagcttg ctttcatcaa
     6241 aaatctgtac atatgcttgt acataatgta caaaacagca gaggtgtttt gcgatttgta
     6301 caaggtgagt aatggcgctg tctgatgtgt ggttgcgttc agtcgttgga aaggaacgtg
     6361 ataaggtttt ggttaaatcc gatcgtgatg gtctgtctgt cagagtatca ccgaaaggtc
     6421 gcgtagtgtt ccaatatcgt tatcaatggg cagggaaagg tgagcgtctt gatatcggaa
     6481 cttacccggc aactggatta aaagaggcca gagaagaagt tatccgtctt cgtggtgaac
     6541 tcgagtcaaa ccgtaatcca cgattggtca agcaggctga aaaacgaaaa gttactgaag
     6601 ccatgacggt agagtctgtg atccgtgctt ggtatgaagc atattgtgta aaaaataaaa
     6661 aaggttctga acagatactt cgctcgtttg agctgcacct gttctctaaa atcgggaata
     6721 tccctcacga tgcagctaca ttgcatgatt ggttagaagt cttggagcct cttagcacta
     6781 agactccagc aatagcagac cgattgctaa ttaacgcaaa gcaggcccat gtctgggcgt
     6841 ataagagaaa gctcattgaa actcgcccgc tgtcggatat cacgggtaaa gatatggata
     6901 tccgtaaagg tcagaagaaa cggtttctga cacatgatga aattaaaatc ctttatgctg
     6961 cgatcgatgg ttctcgaatg gttcctaaat accgggcctt cattaaacta ttgctgcatt
     7021 ttggttgccg tagttcagag ctaattaccg cccgggtgga cgattttgat ttcattaata
     7081 aagtatggac tgtaccacca gaacgacata agactgggga gataacaggc gaaccgctaa
     7141 agcggcccat tattgaaccg gttgaagagc ttataaagta cgctatctct atgaacaatg
     7201 gttccgatat gctttttact aaggaaggaa gcagggagcc cgttggtcgg acatcattgc
     7261 agtcgctgcc ttacaattta atgcagtacg catggcggtg tttaggatat caattccctc
     7321 attggtctct tcatgacttg agacgtacag ctcgaacaaa tttttctgat cttactgcgc
     7381 ctcatatcgc agaaattatg cttggtcata agctgccagg ggtatggcaa gtttatgata
     7441 agagtgatta tctagaagaa cagcgtaaag cttacctggc atggtggaag agggttgaat
     7501 cgattgttaa ttgtactatc tctgattcaa actgacggtt tgtatcacct gaacataacc
     7561 aaatctccta gcacactttg agcgagatgt gagcgttaag tttacgccat tgtgttgaca
     7621 taagaggggg cgggtatcga tatcattact atccctatgc ccctaaatcg ctcctgccat
     7681 ttgataaatg cagatgttta ccaccaaatg tttttgatac tataggtcga ttcatgattt
     7741 acctagaaac ttagttgtaa cgaaaacttc ttaacggaca ctatctggat aaagttagtt
     7801 ttttatttaa gagggttaac atgtcaaagt taaaaaatga accattagta agggtatcag
     7861 aagaagacgg aatagaaata aggaagatcc aatatccaga taatacgata gaaagaattt
     7921 ataagcagaa aggtgtaata ttacca
//
LOCUS       DAZFKF010000170         7912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFKF010000170 DAZFKF010000000
VERSION     DAZFKF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.172"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(175..288)
                     /locus_tag="ACHKA1_004902"
                     /pseudo
     CDS             complement(175..288)
                     /locus_tag="ACHKA1_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012539895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            968..2203
                     /locus_tag="ACHKA1_004903"
     CDS             968..2203
                     /locus_tag="ACHKA1_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5682442.1"
                     /translation="MGKALNKLSDSTLKKLAAVQAEKERFYSDGGGLEIKHSKGGKLT
                     WYFRYRTGGREVAAERLKLGAYPELSLKAAREKRTQCRSWLAEGKNPRYELCATVQEA
                     LKPVTVKEAINYWLEEYAKDNRKDYIKLVQRMDKHIICHIGAIPLDKCDTRQWIACFD
                     RVRKKAPVAAGHVMQTCKQALKFCRRRRYAFSNALDDLIVSDVGKRAEIRERVHSNSE
                     LKEILRAIDGDVFAPYYSALMRLLIVFGCRTAEVRLSEIKEWDLKEMLWTVPKEHSKT
                     KVTIFRPIPDGILPFIQKLVEQNRHTGLLLGELKKDTTVAQYGRNAHKRLNQEHWSLH
                     DFRHTFTTMMNDLGVDPHIVEHITAHQMPGQQKTYNHSRYLQAKRDALNLWVDRLELL
                     QNNDEKIVVMIPRIYTQNS"
     gene            2612..2806
                     /locus_tag="ACHKA1_004904"
     CDS             2612..2806
                     /locus_tag="ACHKA1_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5682443.1"
                     /translation="MREINEDRVIREKECRELTGVCRTTRYEMEKQGRFPSRISLGGR
                     SVGWIKSEVVAWVKNRERVN"
     gene            complement(2965..3192)
                     /locus_tag="ACHKA1_004905"
     CDS             complement(2965..3192)
                     /locus_tag="ACHKA1_004905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682444.1"
                     /translation="MTNNTLSPIQDTQTQDDEIIRQRQSEACARLEEELTRTKIPPPA
                     PRLVPPEKFALEDFVEKYPRRLKSGKNRPPG"
     gene            complement(3250..3429)
                     /locus_tag="ACHKA1_004906"
     CDS             complement(3250..3429)
                     /locus_tag="ACHKA1_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5682445.1"
                     /translation="MERDHINNKSQKLQARAPHEVVEAMEQVKETGESTAQFIVTAMR
                     GEIKRRQRRKAKESE"
     gene            3626..3826
                     /locus_tag="ACHKA1_004907"
     CDS             3626..3826
                     /locus_tag="ACHKA1_004907"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5682446.1"
                     /translation="MRNHTTHPQGRDSHNLNKFTWRFMQCLTDSIAIFTVTAATEREA
                     RAQLPHAHLIFVARIRQGVCHA"
     gene            3819..4139
                     /locus_tag="ACHKA1_004908"
     CDS             3819..4139
                     /locus_tag="ACHKA1_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682447.1"
                     /translation="MPDMSNYQYLINPHFKCDHDIAKKVYSAADGATDNISMGIASIG
                     SLMFHASENEEYDEKAMRIDMGNIGLLLAMLGHFDISLRCTIENATDALNAIKKANTD
                     SNRG"
     gene            4146..4445
                     /locus_tag="ACHKA1_004909"
     CDS             4146..4445
                     /locus_tag="ACHKA1_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5682448.1"
                     /translation="MRTYLSGLTASGYAHPKIIPGAIYRDKNGNRVTVKSIMFDRVYF
                     IRDGYSFHSSLNVEIFICRFRREIPPSRNNHVSCMDVDKKLQELKNMIAAWREQK"
     gene            4442..6259
                     /locus_tag="ACHKA1_004910"
     CDS             4442..6259
                     /locus_tag="ACHKA1_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO5682449.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVWLGEQQLAELDNLQIVPDGRYRVRLYQAGLLRSGLVNTIGQKLAAAGVRDADYY
                     PEGMHSQKRENWREYLERERGDLTEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAINADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGDFREHDKNDWLLIASELPFTPPAEG
                     ETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDS
                     MLAEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCGYLM
                     ASVMCDGLLVGNAEIVPFSPRRYLYHAYLAYMKAHGFGKPVTLTRFGKDMPGAMAEYG
                     REYMKRKTKHGLRSNVTLTEESEDWMPSCVSVTNDDSKN"
     gene            6547..6792
                     /locus_tag="ACHKA1_004911"
     CDS             6547..6792
                     /locus_tag="ACHKA1_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682450.1"
                     /translation="MTAKHTKKSQSHALDLTEHWLRVSIKIIDRNAGEGYAKAHPELI
                     SAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            6789..7094
                     /locus_tag="ACHKA1_004912"
     CDS             6789..7094
                     /locus_tag="ACHKA1_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682451.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            7104..7514
                     /locus_tag="ACHKA1_004913"
     CDS             7104..7514
                     /locus_tag="ACHKA1_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5682452.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDGIPF"
     gene            7525..7797
                     /locus_tag="ACHKA1_004914"
     CDS             7525..7797
                     /locus_tag="ACHKA1_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5682453.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMLLVETDKERHQVTMRRL
                     ECSRKAQKPPEPPDNFGDLRKAVDRTYAEMGIDGVSDEIWRNYPDS"
     gene            7844..>7912
                     /locus_tag="ACHKA1_004915"
     CDS             7844..>7912
                     /locus_tag="ACHKA1_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707048.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682454.1"
                     /translation="MAHYRVFVTVRHSYYLKQTDTTE"
BASE COUNT         2212 a         1663 c         2049 g         1988 t
ORIGIN      
        1 tgaaagcgtt ctttgtcggt ttatccggca agataaactc cagtattaaa ccatgtttct
       61 cagtcccgtt cagacagcct cagaaagata cattccggac cattacccat gtgcatcaag
      121 agtcattttc tttttaatat aagaagccct catgccgcct aatctggact ttcagttgta
      181 gtagatggct tcagagatac cggcatcccg ggagacatct ttaacggttt gtccggattc
      241 aatcgatata atcacggcgt gatgttcagt aatacgggct ttatgcatgg cgctcccctt
      301 cattggcaga ttgattatgc ctgaggatct ctaaatgcga attgcacgat tatacgaagt
      361 acttaacaac gttttataga agcttacgct ccaacctctc tatctgtagt ctgttacaga
      421 ctaatgatgc agtaatctgc aaaaaatgac caaccaataa tggaatatca ctacaggtta
      481 atcactattg tcagatacaa actgcattta tggtataaag gtatgtgatc ttttctatcc
      541 atacttggtc cggcgtgata atactgaagc cttcttacaa aaggttgtat gatacaggct
      601 ggtcatattt atatgacccg attctctgct ggctacgcca gctaccacgt aagccgggaa
      661 aatgacttga gggtaaattt gcatctgcaa gccacttccc ctaatctagc acaaaatata
      721 gagattcaac caaacgactc ggggtgccct tctgcgtgaa ggctgagaaa tacccgtatt
      781 ttctcattta gtgttttttg cggttcgggg gtgttcgtgg ttgtttgtgt atgtatttga
      841 tattaaatga attattctat tttcttgttt gttgttgttc gtcgttgttc gtagagcttc
      901 aacaaaatgt gtggtcagtt gtgtggtcag ttttttgagg ctggaggact gaaaagagga
      961 tctgatagtg ggaaaagcac taaacaaact gagcgattcg acgttaaaaa aattggcggc
     1021 tgtccaggca gaaaaagagc gtttttactc cgacgggggc gggctggaga ttaaacactc
     1081 aaaaggcggc aaattaacct ggtattttcg gtaccgaacg ggaggccgtg aggtcgccgc
     1141 agagcggtta aagctggggg cttaccctga gctgtcgctg aaagctgcta gggaaaaacg
     1201 cacacagtgc cggtcatggc tggctgaggg taaaaatccc cgttatgagt tgtgcgccac
     1261 agtacaggaa gcattaaaac ccgttacggt gaaggaggcg attaactact ggctggagga
     1321 atacgcgaag gataatcgca aagactacat aaaactggtg caacgtatgg ataagcacat
     1381 catttgccat ataggggcaa tccctcttga taaatgcgat acaaggcagt ggatcgcatg
     1441 ttttgaccgt gtacgaaaaa aagcaccagt agcagcaggg cacgtcatgc aaacctgcaa
     1501 acaggcgcta aagttctgcc gcaggaggcg ctacgcgttc agtaatgccc tggacgattt
     1561 gatcgtcagc gatgtgggta agagagcaga aattcgcgag agggtacaca gtaacagcga
     1621 attaaaggaa attttacgcg ctatcgatgg tgatgtgttc gcgccttact acagcgcgtt
     1681 aatgcgcttg ctgattgtgt tcgggtgcag aacggcagag gtcagacttt cagagatcaa
     1741 agaatgggat ctgaaagaaa tgttgtggac agtgccaaaa gagcacagca aaacgaaggt
     1801 aacgatattc aggccaatcc ccgatggcat tttgccgttc attcagaagc tggtggagca
     1861 gaaccgacat acagggttat tgctcggaga actgaaaaaa gataccacgg tagcgcaata
     1921 tgggcgaaat gcgcataagc ggcttaacca ggaacactgg tcgctgcatg actttcgcca
     1981 cacgtttaca acgatgatga acgatttagg tgtcgatccg catatcgtgg agcacatcac
     2041 agcgcatcag atgcccggtc agcaaaaaac ctataaccat tcacgctatt tacaggcaaa
     2101 gcgtgacgcc cttaatctat gggtagaccg tctcgagctt ctccagaaca atgatgaaaa
     2161 aatcgttgtt atgatcccgc gaatttacac ccaaaattct tgacaaatta cggctgtttt
     2221 tcttgcgaaa aatggccctc taaaataaat cgtcgaatat cggcaaatat cgacgattta
     2281 tatgtgtcaa gtattgaaaa aatttgaaat gtcttcttct gagttcttat tctctgtttc
     2341 ttgattgttc caacacctga tggtttatct atttgttttt aatgaggtaa ctcaggtgtc
     2401 atgccaaatg acgacatttt ttgttgcgtc gcttaaaaac tcgtgacacg atttcatgaa
     2461 cagcagcaaa ctctagagaa ctttgacgaa cataaagttc agaaaggtta cgtattcaat
     2521 ttaagaggtg aaaaaagcta agaagtaatc ttttcccgta tatccacagc gaaacaaaaa
     2581 tttctatacg taattttatg tagggctttt tatgcgcgaa ataaatgaag atcgtgtaat
     2641 ccgcgaaaaa gaatgcagag agctaacagg cgtttgccgc acaactcgtt acgagatgga
     2701 aaaacaaggc cgctttccct cccgtatcag tcttggcggt cgttcggttg gctggattaa
     2761 gtctgaggtt gtcgcctggg tcaaaaaccg cgaacgcgtc aattaagaat attcattctt
     2821 taaattccgt ccgtgttttt gtttgtactt gttcgcggtt gttcgtgaag aaaagaagcg
     2881 gcgcgtatgc accgctaagg agtttatttt catgacttat gcagctttca atcttagcac
     2941 cgccgtagct ggtggacaag gctatcagcc aggcggtcgg tttttgccgg atttaagccg
     3001 ccgtgggtat ttctcgacaa aatcttcaag ggcaaatttt tctggtggca ctaagcgcgg
     3061 cgctggtggt ggtatttttg ttctggttag ttcttcctca agtctggcgc aggcttctga
     3121 ctgcctttgc cggatgattt catcatcttg cgtttgcgtg tcttgtattg gtgatagtgt
     3181 gttgttggtc atgatactgc cctgtaaagc aatgcgccgg agtacctcac accacggcgc
     3241 tgatagtgat tattctgatt ctttggcctt acggcgctgg cggcgtttga tctcgcctcg
     3301 catggctgta acgataaatt gcgcagtgct ctcgcctgtt tcttttactt gctccatagc
     3361 ctcaacaact tcatgcgggg cgcgagcctg taatttctgt gatttgttat tgatatggtc
     3421 tctttccatt tctggttccc tttgtgatta ctggaatcca gtatacacga aaaagaccaa
     3481 aacaaaacgc ttgaagtgga ttccacttag tgattatact ggaatccagt tgagtgttta
     3541 tcactcaatg caaacgaggc tgaaaaggat tgccgtcctt tcgcagcctc tgaccaccaa
     3601 cgatagcgaa agtatcgagg tagctatgag aaatcatacc acacacccgc aagggcggga
     3661 ctcgcacaac ctgaataaat tcacctggcg ctttatgcag tgcctgacag acagcattgc
     3721 aatattcacc gttaccgctg ccactgaacg cgaagcccgc gcccagttgc cgcacgcaca
     3781 tctgattttt gtcgcccgta ttcgtcaggg ggtgtgccat gcctgatatg tcaaattacc
     3841 agtacctgat taatccgcat tttaagtgtg atcatgatat tgctaagaag gtttattccg
     3901 ctgctgatgg ggctactgac aatatatcaa tgggtattgc gtcaattggt agccttatgt
     3961 ttcatgcgtc agaaaatgag gaatatgacg aaaaggccat gcgcattgat atgggtaata
     4021 tcggtttgtt actggcaatg cttggacatt ttgatatttc gttacggtgc accattgaaa
     4081 atgccacaga tgcattaaat gccataaaga aagcgaatac tgattcaaat cggggataaa
     4141 taatcatgag aacgtattta tctggcttga ctgccagcgg ttatgcacat ccaaaaatta
     4201 tccccggcgc tatttatcgg gataagaacg gcaacagagt aacagtaaaa tcgataatgt
     4261 ttgaccgtgt gtattttatt cgtgatggct attcatttca tagctcgctg aacgtggaga
     4321 tctttatttg cagattccgg cgggaaattc cgccttccag aaataaccat gtgtcatgta
     4381 tggatgttga taaaaaacta caggaactga aaaacatgat tgccgcgtgg agagagcaga
     4441 aatgaaaaaa gcgccaaatt taaaacacca gccacgtgac aaaatgacgg aagtcatcat
     4501 ttttgcgggt agtgatgcgt gggcacatgc gaagcagtgg caggaacagg acgggcgact
     4561 ggctggcgat aacgtgccac ctgtctggct tggagagcaa caacttgccg aactggacaa
     4621 cctgcaaatc gtaccggacg gacgctatcg cgtgcgtctc tatcaggcgg ggttattgcg
     4681 ttcggggctt gttaatacca tcgggcagaa actggcagcg gcaggtgtca gggatgctga
     4741 ttattaccct gaaggaatgc acagccagaa acgggagaac tggcgcgaat atctggaacg
     4801 tgagcgggga gatctgacgg aaaagaaaaa ggtagttgaa ctgcctgtaa agaaaaaaga
     4861 gcgggtaaaa gacgataacg cttcatcact ggcgcttaac cagatgggag caagtcaacg
     4921 cggcgaagtt ctcctggcac attatggcgg tgaactggcg attaatgctg actctgacac
     4981 tgttcaccat tacaacggcg ttgtatggga gccagtacag gataaagaat tacagcgagc
     5041 tatggcacag attttcattg atgcggagat cagctattcg cagaacgcca ttaaatcggc
     5101 ggtcgatacc atgaagttaa gtttgcctgt aatggggaat acagcccgta acctgattgg
     5161 attcagtaac ggggtatttg atacccgtac aggtgatttc cgtgaacacg ataaaaatga
     5221 ctggctgtta attgccagtg agttaccgtt cactccacca gcagaaggtg aaacgctggc
     5281 aacacatgcg ccgaatttct ggaagtggtt acgccgttcg gtggctgaga atgaccgcaa
     5341 ggcagatcgc gttctggcag cgttatttat ggtgctggcg aaccggtacg actggcagtt
     5401 attcattgag gtaacaggtc cagggggaag tggtaaaagc gtgatggcgg agatttgcac
     5461 catgctggcg ggtaaggcca acacagtatc ggcaagcatg aaggcgctgg aagatgcaag
     5521 ggaacgcgcg ttagtggttg gcttttcgct gattatcatg ccggatatga cccgctacgc
     5581 tggtgatggg gcaggaatta aggccattac aggcggtgac aaggtggcaa ttgacccgaa
     5641 acacaaagcc ccctactcaa cgcgtattcc ggcagtagtg ctggcggtta acaataacgc
     5701 catgtcattc agtgaccgca gcggggggat ctcgcgccgt cgggtgatat tcaatttttc
     5761 ggaagttgta ccggagaacg aacgcgattc aatgctggcg gaaaaaatag aaggtgagct
     5821 ggcggtagtg attcgtcatc tgcttacacg gtttgctgac caggacgaag ccagacgcct
     5881 tttatatgag cagcagaaat cagaagaagc actcgcgata aagcgtgaag gtgattcgct
     5941 ggtggacttc tgcggctatc tcatggcgtc ggtaatgtgt gatggcctgt tagtgggtaa
     6001 tgctgaaatt gtgccattca gcccgcgcag gtatctctat catgcctatc tggcttatat
     6061 gaaggcacat gggtttggta aacctgtaac actgacgcgc ttcggtaaag atatgccggg
     6121 ggcaatggcg gaatatggca gggagtatat gaaacggaaa acgaagcacg gtttgcgttc
     6181 aaacgtgaca ctgacggagg aatcagaaga ctggatgccg tcatgtgtat cggtcactaa
     6241 tgacgatagc aaaaattaaa cttatggaat aactgttcac cactgttcac cctgtcataa
     6301 atatctttta tatcagtata ttatagggtg aacagttatt tatgaactgt tcaccaaact
     6361 attcactgtt cacctttttg attgtttatt gagcttcaag ggtgaacagt ggtgaacagt
     6421 tggtgaatag tttttgtgaa actgttcacc ccttaacatt atgaattaaa agataaaata
     6481 tcaaaaggtg aacaggtgaa gggttaaaac gcaaaaattt taatttactg ctgtgagata
     6541 aagcctatga cagcgaagca cacaaaaaaa tcacaatcgc acgcccttga tttgacggaa
     6601 cactggttaa gggtgtcgat aaaaatcatc gaccgcaacg ccggggaagg atatgcgaaa
     6661 gcacatcccg aactgattag cgcattcatg acaacggcag ctgcaaactt tgccacgctg
     6721 acagaacggg agattgccga agcggaacag gtaacaacca tcaacgttaa aaccggagag
     6781 gtggaatcat gagcgaacca aaatttggtg aaaaatttta caagcacaac ggacggatca
     6841 cgattctgca aataagcgca gctacaccag gctggtgggt tgaaacagac gaaggatcct
     6901 cacctgtggc atcatgggcg ctttgtgtgc ttagttaccc tgaccgcgat gtgtatcagg
     6961 atattctgcc tgtgatatcc actgacaaag gcatgaaacc cgtagacatc aaaaacatgg
     7021 gttttcagtg tgtaatgctc actgaaaaaa tgatggaaga gatgaaaaag aacaacgccg
     7081 gatcgcttca ctgaggtggc aatatgacag cacaaattgc agcttacggg cggctggtgg
     7141 acgacccgca ggtaaaacag accagtaagg gcacaccgat gacgctggcg cgtatggcgg
     7201 tatcgttgcc atgtagtcag gcgcaggatg ggcaggcgac gttatggcta tcggtcatcg
     7261 catttggtaa gcaggccgac ttcctggcta aacatcaaaa aggcgacgtt gccagcgtat
     7321 ccggcacgat gcaggtcagc cagtggaccg gacagaacgg ggaaacgcgg cagggttatc
     7381 aggttattgc agacagcgta atcagtgccc gtgcggcacg tcctggcggg aacagacgca
     7441 aaaccacagg cacacagggt aatcagccac cagcgggagg cgatgaccct tacggtgacg
     7501 gtattccgtt ctgagggggt ggcgatggta catgaccgca tagcggaaga actcgaggcg
     7561 aaaggctttt accggagggc ggcggcgcga tggggtgaag tcatgctgct ggtggagaca
     7621 gacaaggaac ggcatcaggt tacgatgcga cggctggaat gttccaggaa ggcacagaag
     7681 ccaccggagc cgccggataa cttcggagac ctgagaaagg cagtagatcg cacttatgct
     7741 gaaatgggta tagatggtgt aagcgatgaa atatggcgta attacccaga cagctaatca
     7801 acagccggag aaatccggct ttttttgcac cagttgaaac ggtatggcgc attaccgggt
     7861 tttcgtcacg gtcaggcata gttactatct gaaacaaaca gacacaacag ag
//
LOCUS       DAZFKF010000171         7618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFKF010000171 DAZFKF010000000
VERSION     DAZFKF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.173"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..230
                     /gene="yafQ"
                     /locus_tag="ACHKA1_004916"
     CDS             <1..230
                     /gene="yafQ"
                     /locus_tag="ACHKA1_004916"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO5682455.1"
                     /translation="LAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRD
                     AHVEPDWLLIYKLTDKLLRFERTGTHAALFG"
     gene            386..1126
                     /gene="dpaA"
                     /locus_tag="ACHKA1_004917"
     CDS             386..1126
                     /gene="dpaA"
                     /locus_tag="ACHKA1_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO5682456.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1097..1864)
                     /gene="yafJ"
                     /locus_tag="ACHKA1_004918"
     CDS             complement(1097..1864)
                     /gene="yafJ"
                     /locus_tag="ACHKA1_004918"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO5682457.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETSNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2070..2648)
                     /gene="lpcA"
                     /locus_tag="ACHKA1_004919"
     CDS             complement(2070..2648)
                     /gene="lpcA"
                     /locus_tag="ACHKA1_004919"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO5682458.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2888..5332
                     /gene="fadE"
                     /locus_tag="ACHKA1_004920"
     CDS             2888..5332
                     /gene="fadE"
                     /locus_tag="ACHKA1_004920"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO5682459.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAHSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYKAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKDLGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5375..5821)
                     /gene="ivy"
                     /locus_tag="ACHKA1_004921"
     CDS             complement(5375..5821)
                     /gene="ivy"
                     /locus_tag="ACHKA1_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO5682460.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6002..6772
                     /gene="yafV"
                     /locus_tag="ACHKA1_004922"
     CDS             6002..6772
                     /gene="yafV"
                     /locus_tag="ACHKA1_004922"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO5682461.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLW"
     gene            complement(6830..7198)
                     /locus_tag="ACHKA1_004923"
                     /pseudo
     CDS             complement(6830..7198)
                     /locus_tag="ACHKA1_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(7244..>7618)
                     /locus_tag="ACHKA1_004924"
     CDS             complement(7244..>7618)
                     /locus_tag="ACHKA1_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO5682462.1"
                     /translation="KGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATA
                     NRNHWHVENKLHWRLDVVMNEDDCRIRRGNAAELFSGIRHIAINILTNDKVFKAGLRR
                     KMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         1799 a         1927 c         1983 g         1909 t
ORIGIN      
        1 aacttgcaca aaagcgtcat aaggatatga ataaattgaa atatcttatg acgcttctta
       61 tcaataatac tttaccgctt ccagctgttt ataaagacca cccgctgcaa ggttcatgga
      121 aaggttatcg cgatgctcat gtcgaaccgg actggctcct gatttacaaa cttaccgata
      181 aacttttacg atttgagaga actggaactc acgcggcgct ctttgggtaa agcccgctgc
      241 cgatgaaatt tgtaaaccct gcgccgacag gcttgcgtaa aactcttttg gtgataatgt
      301 gagccttcca aatccagact attccgattt gggatccatt ttgccgtcct ggcgtgtgta
      361 accgttttat caaggaataa gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt
      421 gatcccgtgc gtttcgtttg ccggtttgct gggcagcagt agctcgacca cgccagtgag
      481 caaagagtat aagcagcagt tgatgggatc ccctgtctac atccagatct tcaaggaaga
      541 acgtacgctc gatctctacg tcaaaatggg tgagcaatat caactgctcg acagctataa
      601 aatctgtaaa tattccggcg gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc
      661 ggaagggttt tatagcgtcc agcgtaatca gttaaaacca gacagccgtt actacaaagc
      721 gattaatatt ggtttcccca atgcctatga ccgtgcgcat ggttatgaag ggaaatacct
      781 gatgatccac ggcgattgtg tttccatcgg ctgctacgca atgaccaatc agggtattga
      841 tgagatcttc cagttcgtta ctggtgcgct ggtgtttggt cagccgagcg tgcaagtgag
      901 tatttacccg ttccgcatga ccgacgctaa tatgaagcgc cacaaatatt ccaactttaa
      961 ggacttctgg gagcaactga agccaggcta cgactacttt gagcaaaccc gtaagccacc
     1021 gaccgtttct gtggtcaatg gccgttacgt ggtcagcaag ccgttaagcc acgaagttgt
     1081 gcaaccacaa ctggcatcaa actacacgct ccccgaggca aaataagcgc cattcgcctg
     1141 gcataatctt ttgccaggtt tcattgcccg tcagcggctg tgtcgcaatc accgtgacca
     1201 catcattcgg tgtggtctgc gagctgaagt cgatttctac atcctgatcc agcaacgttg
     1261 ccacgccaaa cggcgcgcgg cgggtgatcc agtgtaaatt agtcgagcaa tacgccatta
     1321 catagcgacc gtccgaaagc agcatgttga aaacgccctt cgcccgcagt tcatccgcca
     1381 gtgaggcgat atatttaaac actgccgcca tgttgcccgg cgtgcgcggg taacgctgcg
     1441 ttaatttatg caggagccag caaaaggctt tttcgctgtc ggtttcaccg accgggcgga
     1501 agttgctggt ttccagtgat ttgtagcccg tcagttgccc gttatgggca taagtccagt
     1561 tacgccccca taactcgcgg gtaaacgggt gagtattttc cagcgccacc tcgccccgat
     1621 tagcctggcg aatatgagcc accaccgaac aggattttat cgggtagtcc tggaccagtt
     1681 tggcgatagg tgagttaaag ctgggttgcg gatctttaaa tgtgcgacag cctttacctt
     1741 cgtaaaaggt aatgccccag ccatctttat gtggcccggt tccaccacca cgctgcacca
     1801 gaccggtgaa actaaagcag atatcggtag ggacgttggc gctcatcccg agcaattcgc
     1861 acatgggtaa cctccacaac agcgggagcg catggccccc ggcgaagatt gatttaagaa
     1921 ttttcaggtc ggatgcggcg tgaacgcatt attcggccta cggttcgcgt acagatttgt
     1981 aggcatgata agacgcgtca gcgtcgcatc aggcatcggt gcacaattgc cggatgcggc
     2041 gtaaacgtct tatccggcct acgccagact tacttaacca tctctttttc aatcaactgg
     2101 atcaggatat ggatcacttt aatgtgaatc tcctgaatgc ggtcggcata accaaagtgc
     2161 ggtacgcgaa tttcgatatc cgccgtgcca gccattttgc cgccgtcttt accggtcagg
     2221 gtgatcactt tcattccctt ctcacgcgcc gctgcgatcg ctttgatcac gtttgcagag
     2281 ttaccggagg tggagatccc cagcagtaca tcgccttcgc gacctaccgc ttcaacgtaa
     2341 cgggagaaaa tatcattgaa accgaaatcg ttaccgacgc aggaaatatg actaacgtca
     2401 gaaatagcaa tcgccgggta gcccggacgg ttttcacggt agcgaccggt caactcttcg
     2461 gcaaagtgca tagcgtcgca atgggaaccg ccgttgccgc aggaaagcac tttgccaccg
     2521 gctttaaagc tgtctgctaa caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca
     2581 tcttttaaaa agttagccag cgtttccgcc gcttcgttca gttcgttacg aataagatcc
     2641 tggtacatga ggatatcctt cagcataaat gtaatagaca aaatgcagtg taccggatac
     2701 cgccaaaagc gagaagtacg ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg
     2761 ataaaaacag caacaatgtg agctttgttg taattatatt gtaaacgcat tgctaaatgt
     2821 ttttacatcc actacaacca tatcatcaca agtggtcaga cctcctacaa gtaaggggct
     2881 tttcgttatg atgattttga gtattctcgc tacggttgtc ctgctcggcg cgttgttcta
     2941 tcaccgcgtg agcttattta tcagcagtct gattttgctc gcctggacag ccgccctcgg
     3001 cgttgctggt ctgtggtcgg cgtgggtact ggtgcctctg gccattatcc tcgtgccatt
     3061 taactttgcg cctatgcgta agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa
     3121 ggtgatgccg ccgatgtcgc gcactgagaa agaagcgatt gatgcgggca ccacctggtg
     3181 ggagggcgac ttgttccagg gcaagccgga ctggaaaaag ctgcataact atccgcagcc
     3241 gcgcctgacg gctgaagagc aagcgtttct cgacggcccg gtagaagaag cctgccggat
     3301 ggcgaatgat ttccagatca cccatgaact ggcggatctg ccgccagagt tgtgggcgta
     3361 ccttaaagag catcgcttct tcgcgatgat catcaaaaaa gagtacggcg ggctggagtt
     3421 ctcggcttat gcccactctc gtgtgctgca aaaactctcc ggcgtgagcg ggatcctggc
     3481 gattaccgtc ggcgtgccga actcattagg cccgggcgag ctgctgcaac attacggcac
     3541 tgacgagcag aaagatcact atctgccgcg tctggcgcgt ggtcaggaaa tcccttgttt
     3601 tgcgctaacc agcccggaag cgggttccga tgcgggcgcg attccggaca ccgggattgt
     3661 ctgcatgggt gaatggcagg gccagcaggt gctgggcatg cgactgacct ggaacaaacg
     3721 ctacattacg ctggcaccga ttgcgaccgt gctgggtctg gcgtttaaac tctccgaccc
     3781 ggaaaaatta ctcggcggtg cagaagattt aggcattacc tgtgcgctga tcccgaccac
     3841 cacgccgggc gtggaaattg gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg
     3901 accgacgcgc ggtaaagatg tcttcgtacc gatcgattac attatcggcg gaccaaaaat
     3961 ggccgggcaa ggctggcgga tgctggtgga gtgcctctcg gtaggccgcg gcatcacact
     4021 gccttccaac tcaaccggcg gcgtgaaatc ggtagcgctg gcaaccggcg cgtatgctca
     4081 cattcgccgt cagttcaaaa tctctatcgg taagatggaa gggattgaag agccgctggc
     4141 gcgtattgcc gggaacgctt atgtaatgga tgctgcggca tcgctgatta cctacggcat
     4201 tatgctcggc gaaaaacctg ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg
     4261 cgggcagcag tcgattattg atgcgatgga tattaccggc ggtaaaggca ttatgctcgg
     4321 gcaaagcaac ttcctggcgc gtgcttacca gggcgcaccg attgccatca ccgttgaagg
     4381 ggctaacatt ctgacccgca gcatgatgat cttcggacaa ggagcgattc gttgccatcc
     4441 gtacgtgctg gaagagatgg aagcggcgaa gaacaatgac gtcaacgcgt tcgataaact
     4501 gttgttcaaa catatcggtc acgtcggtag caacaaagtt cgcagcttct ggctgggcct
     4561 gacgcgcggt ttaaccagca gcacgccaac cggcgatgcc actaaacgct actatcagca
     4621 cctgaaccgc ctgagcgcca acctcgccct gctttctgat gtctcgatgg cagtgctggg
     4681 cggcagcctg aaacgtcgtg agcgtatctc ggcacgtctg ggtgatattt taagccagct
     4741 ttacctcgcc tctgccgttc tgaagcgtta tgacgacgaa ggccgtaatg aagccgatct
     4801 gccgctggtg cactggggcg tacaggatgc gctgtataag gctgaacagg cgatggacga
     4861 tttactgcaa aacttcccga accgcgtggt tgccgggctg ctgaatgtag tgatcttccc
     4921 gaccggacgt cattatctgg caccgtctga caagctggat cataaagtgg cgaagatttt
     4981 acaagtgccg aacgccactc gctcacgtat tggtcgcggt cagtacctga cgccgagcga
     5041 gcataatccg gttggtttac tggaagaggc gttggtggat gtgattgccg ccgacccgat
     5101 tcatcagcgg atctgtaaag atctgggtaa aaacctgcca tttacccgtc tggatgaact
     5161 ggcgcacaac gcgctggcga aggggctgat tgataaagat gaagccgcta ttctggtgaa
     5221 agcggaagaa agccgtctgc gcagtattaa cgttgatgac tttgatccgg aagagctggc
     5281 gacgaagccg gtaaagttgc cggagaaagt gcggaaagtt gaagccgcgt aattgaataa
     5341 cggagccgaa aggctccgtt tctttatccg ctaattattt aaaattaaag ccatccggat
     5401 ggttttccag gctgccggtc aacgccgcga acagcaccgt tttaccatca atcgaaagcg
     5461 catcgttcac attcaaccag gtgagtttct cttgcgacgt tttctcatca atagtcgaga
     5521 acagccccgt catctgatta gatttctcgg accacatcac agcgatacgt tgcgagccac
     5581 agtcatgcgg tttgcacgcg ctcatcacct gatacgtctc atctcccaat gtcacggttt
     5641 gtgcgggagt ataagtaccg cctttcatca cccatgcagg tagcttatgc ccttgcacca
     5701 tctgattaaa cgcagctttg gtggtttcgc cctttgccag gctgctaatg gttaaatcat
     5761 cctgcgccat tgcactggtg gcgatgacca gagcggcgac tgtcgttatt gccttaaaca
     5821 tcattcctcc cgagcttatc ctgcccatgt tattaacctc ctaaagcctg gcgataacac
     5881 gataatttgc aaatcagata tttccaatat cagcatttgt tatcaattta ttctgcgcta
     5941 aggcaactgc ctgataaggt gttaaagtta taaaaccctt cgcataaagg agttgtacac
     6001 cgtgcctggt ttgaagatta cgcttttgca gcaaccgttg gtgtggatgg atggtcctgc
     6061 caacctgcgt cattttgatc gtcaactgga aggtattacc gggcgcgatg tgatcgttct
     6121 accggagatg tttaccagcg gctttgccat ggaagcggca gcttcgtcgc tagcgcaaga
     6181 tgacgtagtg aactggatga cagctaaagc gcagcagtgc aatgcgctga ttgcaggcag
     6241 tgttgcatta caaacggagt ctggttcagt taaccgcttt ttgctggttg agccgggcgg
     6301 cacggtacat ttttatgata agcgtcatct gttccgaatg gctgatgagc atctgcatta
     6361 taaagcgggc aatgcgcgag tgattgtgga atggcgcggc tggcgtattt tgccgctggt
     6421 gtgctacgac ttacgttttc ctgtgtggtc gcgcaatctc aacgattatg acctcgccct
     6481 gtacgtcgcc aactggcctg ctccgcgctc tctgcactgg caggcattgc tgacggcccg
     6541 cgcgattgag aatcaggcgt atgtggcggg atgcaatcgc gtcggcagcg atggcaacgg
     6601 ctgccattat cgcggtgaca gccgggtgat taatccacaa ggcgagatta tcgctactgc
     6661 cgacgcgcat caggcaacgc gcattgatgc ggagctgtcg atgatggcat tgcgggaata
     6721 tagagaaaag tttccggcat ggcaggatgc ggatgagttt aggttgtggt gagtgggggt
     6781 tgtgtttagg gccggggaga gtcggggtat gactacgaaa gcccgctctc ctgcgaccac
     6841 ttttatcata ggagtgtgga agtgcttttc catcaatcgc gatgacgtct ttgtcatttg
     6901 atgaatggca gtcacgcatc cagttaataa agcactcgtg aaattttgca ggactgatac
     6961 aggatacaac tctggcaatg gtatcgtgaa caggaatacc attttcaaaa tcaccatatt
     7021 gcttcaaaaa atcgagatgt gtttccccaa aatcctctat atcttcccaa ccttctgcac
     7081 cagaaataac ggcacaaata gtcaacagta gaatatccga taatttatgt tccacgttcc
     7141 aggcttgtct gtaatcgggg ataacagaaa tatgttccat caatttttta agttccattt
     7201 tgttctcctt aattatgtaa ggagtatttg atccagggca agattacgaa agcccgctcc
     7261 ccgcaaggac tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta
     7321 accctgcctt gaatacctta tcattcgtca aaatattaat agcaatgtgc cgtatccctg
     7381 aaaataattc tgctgcattt cctcttctta ttctgcagtc gtcttcattc attaccacgt
     7441 ccaggcgcca gtgcagctta ttttccacgt gccagtgatt tcggttcgct gtggcgaact
     7501 tctctgcagt taaatcagca gagctgatat aatatctgac cgtcatttcg ggttctttct
     7561 tttgttctgc tattattgac cgaaaggaga ctgccacgca taatttcttc agcccttt
//
LOCUS       DAZFKF010000172         7485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFKF010000172 DAZFKF010000000
VERSION     DAZFKF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.174"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..279)
                     /locus_tag="ACHKA1_004925"
     CDS             complement(<1..279)
                     /locus_tag="ACHKA1_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFO5682463.1"
                     /translation="MFPVKSLSVVLLFLASCTQASTRAEIERLWHPEGMAARTTQPAA
                     DAQVRQEKAATRWFRLSNGTQVNLADWKVVLFMQGRCPYCHQFDPVLKQ"
     gene            complement(266..556)
                     /gene="traQ"
                     /locus_tag="ACHKA1_004926"
     CDS             complement(266..556)
                     /gene="traQ"
                     /locus_tag="ACHKA1_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFO5682464.1"
                     /translation="MISKHRFSLPRLDITGMWVFSLGVWFHIVARLVYRKPWMAFFLA
                     ELIAAILVLFGAYQILDTWIARVSQEEREALEVRQQLLLRERKDNDDEHVSR"
     gene            638..973
                     /locus_tag="ACHKA1_004927"
     CDS             638..973
                     /locus_tag="ACHKA1_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFO5682465.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSFPIKTFA
                     DLFVSILLFIAIIAVVIWLFLIIYVYFCELFRSHWIAVWFILWSVVINLTIGFVIYDK
                     LAPVISLMK"
     gene            complement(954..1295)
                     /gene="trbA"
                     /locus_tag="ACHKA1_004928"
     CDS             complement(954..1295)
                     /gene="trbA"
                     /locus_tag="ACHKA1_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO5682466.1"
                     /translation="MHTGDLCFFTGASLFIVGGVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCVLPDNFLFLCGAVMNQCAVPVVSMAVGTIPVITKFERFRRIFVCIMLSSAWSG
                     VMWFFIISLVK"
     gene            complement(1309..2052)
                     /gene="traF"
                     /locus_tag="ACHKA1_004929"
     CDS             complement(1309..2052)
                     /gene="traF"
                     /locus_tag="ACHKA1_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFO5682467.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEQYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(2045..2302)
                     /locus_tag="ACHKA1_004930"
     CDS             complement(2045..2302)
                     /locus_tag="ACHKA1_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFO5682468.1"
                     /translation="MKVIFTSNRLVDFLIRLVITAIVISPVIIWSWDTVKETTAGGVL
                     AAAFIILYSGVLLFILYGCFSALADLQKPDERKSDERNEDE"
     gene            complement(2316..4220)
                     /gene="traN"
                     /locus_tag="ACHKA1_004931"
     CDS             complement(2316..4220)
                     /gene="traN"
                     /locus_tag="ACHKA1_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFO5682469.1"
                     /translation="MKEKINTFFRSLNVNDSGRPRNICAIVFFLSFSVFADSNSDYRA
                     GSDFARQIQGQGIGSIQSFNPQESIPGYNANPDETQYYGGVTAGGDGGLKNNGTTEWA
                     TGETGKTITESFMNKPKDILSPDAPFIQTGRDVESRADSIVGNTGQQCSAQQINRSEF
                     TNYTCERDTMVEEYCSRTASIIGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTVPV
                     AGTVESASLSAHAGLYFLNSRYDFMNTAFNVQLVTGQSGTFTIPVPSGFSVTQGQVLT
                     GSGCTANGNCLPHGNGDRMVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNK
                     ADGVLKGTECSEPGGTKTGVMEGKTWSITQACWAYRDKYVTQSADNGTCQKYVDNPAC
                     TLASRQCAFYSDEGACLHEYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDF
                     GEAVSQLAALAAAGKDVAALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLA
                     KCNSEEKALGKAKDNKLTVSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNG
                     QLRIGFGSAKSPDCRGITVDELQRIKFDQLDFTNFYEDLMNNQKIPDNGALTEKVKEQ
                     IAGQLKQVGQ"
     gene            complement(4314..4694)
                     /locus_tag="ACHKA1_004932"
     CDS             complement(4314..4694)
                     /locus_tag="ACHKA1_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682470.1"
                     /translation="MSLPAGYYRIEPDIRALVAAMNVHGFRTYASCQGHGFPVTKLLP
                     YIAFACPVKMTALLEQRLRQDAESAIPRLTWGWSVKGAFNSDLQLCFRLQPEGPHCWY
                     HRYCRRSLRADFRTLILLLKSLSE"
     gene            complement(4691..5329)
                     /gene="trbC"
                     /locus_tag="ACHKA1_004933"
     CDS             complement(4691..5329)
                     /gene="trbC"
                     /locus_tag="ACHKA1_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFO5682471.1"
                     /translation="MNKKIYILFALTAGLFLSAHASENVNTPENRQFLKQQETLSQQL
                     REKPDTQLQAWAEQQVRANPLQHADNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGHDIIRGNLRVRQALEKVAATGDCRQVAQGLLDGTGDKLQ"
     gene            complement(5558..5890)
                     /locus_tag="ACHKA1_004934"
     CDS             complement(5558..5890)
                     /locus_tag="ACHKA1_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682472.1"
                     /translation="MEKKLSSKYIHIYMSISCVFIRLTGKALKLFPFALVMLFTVLFY
                     FMPENVICDIISEWQRSDVSDRIRVIRLYLGTTFLCTWAAVFLEFLCSDEFLPGKRLN
                     KNEEENNG"
     gene            complement(5917..6042)
                     /locus_tag="ACHKA1_004935"
                     /pseudo
     CDS             complement(5917..6042)
                     /locus_tag="ACHKA1_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
     gene            complement(6091..6537)
                     /locus_tag="ACHKA1_004936"
     CDS             complement(6091..6537)
                     /locus_tag="ACHKA1_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682473.1"
                     /translation="MSEGTRVNDFAYVKQALLAVFHRMNTRPLTRLCEKDDIQRGIAD
                     IQWMLHHHYYPSPGQVGFIIFLLRDEKFRVVREDGRQSFLAVEIQSLIDVLKDIRKYL
                     QFVTRYDCDGCIIRLHASAERKYLWIFLECVIVFILCAVLFFLIIC"
     gene            complement(7266..>7485)
                     /locus_tag="ACHKA1_004937"
     CDS             complement(7266..>7485)
                     /locus_tag="ACHKA1_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682474.1"
                     /translation="SKASVMLTGILVIPVFLVLSGLLSAGYPGLLCITVSGCYFSGIA
                     AFSLRAKDPVGLCWSVFSFILLYLLLKK"
BASE COUNT         1984 a         1970 c         1548 g         1983 t
ORIGIN      
        1 ttgtttcagt accgggtcaa actggtgaca gtacggacat cgcccttgca tgaacagcac
       61 cactttccag tcagcaagat tgacctgagt tccgttgctg aggcggaacc agcgggttgc
      121 tgctttctcc tgtcgcacct gtgcgtctgc tgccggctga gttgtccgtg ctgccattcc
      181 ctccggatgc cagagtcgct cgatttccgc acgtgtggat gcctgtgtac agctggccag
      241 aaaaagcagg acaacggaca gcgatttaac gggaaacatg ttcatcatca ttatccttcc
      301 gttctctcag taacaactgc tgtctgactt ccagtgcctc acgctcttcc tgactgacgc
      361 gggctatcca tgtatccagt atctggtatg cgccaaacag aacgagaatg gctgcaataa
      421 gctctgccag aaagaaagcc atccagggtt ttctgtaaac cagacgggca acaatatgga
      481 accagacacc cagggaaaat acccacatcc ccgtaatatc cagtcggggt aaagagaatc
      541 tgtgtttact tatcatttga atatcctgtt gtggtttaca tgaatttatt cacgcatata
      601 acctgcttaa ctttcgtgaa attaatcagt gtgttatatg aaaaagttat ctttcaaaga
      661 aaaactggaa atagtgagaa acatcataag agagtcatta tcaggtaatg cggcttttgc
      721 tttactggtg tattcattat tgcattcctt ccccattaaa acctttgctg atttatttgt
      781 gtcgatactg ttatttatag caatcatcgc tgttgtgata tggctatttc taatcatcta
      841 tgtttatttt tgtgaattat tcagaagtca ctggatagca gtctggttta tcctctggtc
      901 tgtggtaatc aacctcacta ttggatttgt catttatgac aaattagcac cagtcatttc
      961 actaatgaaa taataaaaaa ccacattact cctgaccatg ctgatgacaa cataatacag
     1021 acaaatattc ttctgaacct ctcgaacttc gtgataaccg gaatggtacc gactgccatt
     1081 gaaacaacgg gtacggcaca ctgattcata accgcaccac aaagaaacaa gaaattatct
     1141 ggcagcacac atacacttat gacatttact acaaagaaga caaacgagct cagcagataa
     1201 actcctttat tatttggtcc tgtaaagaaa taaccaacac cccccacgat aaacaggctg
     1261 gcacctgtaa aaaaacacaa atcccctgta tgcatatata tcctttggtt aaaaattcgg
     1321 tttaaaatct tcagatacgt tcaggaactg ttttgccagg tcatcctgcg aaataaagcc
     1381 gtatgataat ggacgaacac tgccctgttt cgggtctacc agcatcatgg ccgggaaata
     1441 tttcacgccg aggcgctgcg cctgcccctg gtcagtccgg gaatccggca atagtggatt
     1501 aatcacgcca tccacggaaa cgggaataac actcagacca tacgtatccc ggaagccatt
     1561 aatgacctgc gccagctgcc cgtcgatggg gtcctgcccc cggtaaaaaa acataatgcc
     1621 gtattgttca gccagtttcg caatggcctg tcgctgctgt gcctggtctg ccgccagctg
     1681 gttccgtacc gtgccgttgt aatggctgta ctgcaggtta tagtccagtt caggatgtgc
     1741 cagcatggcc tttttggcgc tcatggtgaa aagcccggcc tgctgagtcc agtaattctg
     1801 cagccggaaa tatttcacaa aattatccac gccgggatac agaatggctt cgtacagcgc
     1861 acgctttgtt gccgtctgca gtgcggccag tttctgcata atatccggct cctgacgggg
     1921 agctgctggt actggtttat tttctttttc cttcggattt attttctcgt tataccactg
     1981 ccagcctgca tctttccctg acgccggaaa aataaagcag cagagtaaca gcggcagtaa
     2041 tgctttattc atcttcattc ctttcatcac tttttctttc atcaggtttc tgcaggtcag
     2101 ccagtgcaga aaaacaacca tacagaataa acaggagcac accggagtaa aggataatga
     2161 aagcagcagc aagcaccccg cctgccgtgg tttcttttac cgtatcccac gaccagataa
     2221 ttaccgggga aatgacaatt gctgtaataa caagccggat aagaaaatca acaagacgat
     2281 tcgacgtgaa tatgactttc attattttcc cgaccttact gccccacctg tttcagctga
     2341 ccggcaatct gctctttcac tttttcggtc agcgcaccat tatccgggat tttctggtta
     2401 ttcatcaggt cttcgtagaa gttagtgaag tccagctggt cgaacttaat acgctgcagt
     2461 tcatccacgg ttattccccg gcagtcagga ctttttgcgc tgccaaaccc gatacgcaac
     2521 tgtccgttgc gcccctgctg ctggactatc tgcgccagct tcgaatcaaa ctggcagtaa
     2581 ctgcgctttt tctgcagaca gacacccagc actttccggg agcagaactc acccacactc
     2641 accgtcagtt tattgtcctt cgcctttccc agcgcctttt cttcactgtt gcacttcgcc
     2701 agacccacat cctgacccca gccaccatcc ttacagcagt tactgaagcc ggctgcggcc
     2761 ttacggcaga atttcgcctc gcccgtgaaa gccctgacat ccacaccatt cagggcggca
     2821 acatccttac ctgcggcggc cagtgccgcc agctgtgata ctgcctcacc aaaatcactg
     2881 ttttttccgc tctgcgcctt gtcgcattca ccgtcaaggc agaagacatc accaccacag
     2941 accatcactt ttcctgatgt tctggactcg caggagtacg tggcatactc atgcagacaa
     3001 gctccctcat cggaataaaa cgcgcactga cgtgaggcca gcgtacaggc cggattatcg
     3061 acatatttct gacaggtgcc gttatctgcg gactgtgtga catatttatc ccggtatgcc
     3121 cagcacgcct gtgtgatgct ccaggtttta ccttccatca cacccgtttt cgtaccaccc
     3181 ggttctgaac actcagtgcc tttcagcacg ccatccgctt tattaaacgg acaactctcc
     3241 acccactcca cccgtggaac ccattcctta tcacggacct tcatcctgag tttcagcgta
     3301 aaggtggaag caccactgac aagcgattca tagaccatcc tgtcaccatt cccgtgcggg
     3361 aggcaattac cgtttgcagt acagccacta ccggtcagaa cctgcccctg tgtgacagaa
     3421 aacccggagg gcacaggtat ggtgaaagtc ccgctctgtc ctgtaaccag ttgcacatta
     3481 aaggctgtgt tcataaagtc gtaacgggag ttaaggaaat atagccctgc atgagccgac
     3541 agcgaggcac tttccactgt cccggcaaca ggtacggtaa cgctgaacac cagtttcaga
     3601 ccgttcatac tgaaccggaa ctgtgagtgc ggtatcgtca cttcacgata ttcatccgtg
     3661 tacttccagt ctccgatgat actggcagtc cgggagcagt attcctccac catcgtgtcc
     3721 cgctcacagg tatagtttgt gaactcactg cggtttatct gctgtgcact gcactgctgc
     3781 ccggtgtttc ccacaatgct gtcagcccgg ctctccacat cccggccagt ctgaataaat
     3841 ggcgcatccg gtgacagaat gtctttcggc ttgttcataa atgactctgt gatggttttg
     3901 ccggtttcac cggtcgccca ttccgtggtg ccgttattct tcagaccgcc atccccgccg
     3961 gcagtcaccc caccgtaata ctgcgtctca tccgggttcg cgttataccc ggggatactc
     4021 tcctgcggat taaaactctg aatactgcca ataccctgcc cctggatctg acgggcaaaa
     4081 tcggaacctg cccggtaatc actgttgctg tcggcaaata cagaaaagct caggaaaaaa
     4141 acaattgcac aaatattgcg cggtcttccg gagtcattaa cattcagaga cctgaaaaag
     4201 gtatttatct tttctttcat aacctgatcc atactggaga atgaagtgat gtcatctcaa
     4261 cttcagaacg gtgttgattt tggtgtatgg agcttcctgg cgtaccggga ttactactca
     4321 gataacgact tcagtaacaa tatcagcgtc ctgaagtctg cacgcagtga acgacgacag
     4381 taccggtgat accagcaatg aggaccttcc ggctgaagcc ggaaacagag ctgaaggtca
     4441 ctgttaaaag cccccttcac cgaccatccc catgtcagac gtggtattgc tgattcagca
     4501 tcctgccgca gtcgctgctc aagaagggca gtcattttta ccggacaggc aaaggcaata
     4561 taaggcagca gctttgtgac cgggaaacca tgtccctgac aggaggcata tgtacggaat
     4621 ccgtgaacgt tcatcgcggc caccagtgca cggatgtccg gttcaatccg gtaatacccg
     4681 gcaggtaaac tcattgcagc ttatccccgg tgccgtccag cagtccctga gccacctgcc
     4741 ggcagtcccc cgtggcggcc actttttcca gcgcctgcct gacgcgcaga tttccccgga
     4801 tgatgtcgtg cccctggctg cagaacacca ccagggccgg cacgctgcgg atgccgtact
     4861 gtgaaaacaa cgtcgggtcg atctgaacac catcggtcgc gccatctttt accagggaca
     4921 acacggcttc agcggtagtc ttcaggtcat tgttcaccat accccgcagt gtggccggaa
     4981 tgccgtagtg cctggtttct cccagcatcc gtttcagtcc ctcttcggga atggagaacg
     5041 acacaaaata cacggccccc tgctgtggtt tgtctttctg agcagcctgc tgtttacgga
     5101 ccagttcatc gagaaactgg ttatcggcat gctgaagggg attcgccctg acctgctgtt
     5161 ctgcccatgc ctgcagttgt gtgtccggct tttcacgcaa ctgctgactc agtgtttcct
     5221 gctgcttcag gaactggcga ttttcgggcg tattcacatt ttccgaagca tgggcagaaa
     5281 gaaacaatcc tgctgtcagg gcgaataaaa tgtatatttt tttgttcata taattctcct
     5341 gcaaacagac agtaattaat cgtcataatg aggtgctcaa tagagcaagc ctggagataa
     5401 ctgacgccat gttaaacaca acgaagatcg ttgattaact tctttctcca tttatctttt
     5461 ttctgagact ggcagcaatt agataaacga taccggatat tcactctgtt aaatcattaa
     5521 ttcatttctg accgatgcac ataaaatgag tcagatatta cccgttattc tcttcttcat
     5581 ttttgtttaa tctctttccc ggcagaaact catcagagca gagaaactcc aaaaaaacag
     5641 ccgcccaggt gcataaaaaa gttgttccca gatatagtct gataactctg atcctgtctg
     5701 acacatcact tctctgccat tcactgataa tgtcacagat aacattttca ggcataaaat
     5761 aaaacaacac cgtgaatagc atcaccagtg caaaaggaaa caattttaat gctttgcctg
     5821 tcaaacgaat gaacacacag gatatactca tataaatatg aatgtattta ctgcttaatt
     5881 tcttttccat ttaaaatatt cctctttttg tttaagttac agaaatacac agttacgttt
     5941 acgccacatc aggtaaccga agtttttctt tgtgtcaggc ggattttttt ccgtctcccg
     6001 gcgcatcaca ctgcgcccga acggatggca ctgtccgctg tccgctgtcc gctgtccgct
     6061 gtccgggtac atgttcacca ccaggtgttt ttaacagata atcagaaaaa acagcacagc
     6121 acacaaaata aacacaatta cacactcaag gaaaatccac aggtatttac gttcagcact
     6181 ggcatgcagc ctgataatac aaccatcaca gtcataccgt gtcacaaact gcaggtattt
     6241 ccgtatatct ttcagaacat caattaacga ctgaatttca actgcaagga atgactgacg
     6301 cccatcttcc cgcactaccc ggaacttctc atcccgcaat aaaaatatta taaaaccaac
     6361 ctgtccgggt gatggataat agtgatgatg taacatccat tgaatatctg caattccacg
     6421 ctgaatatca tctttttcac atagcctggt gagcggccgg gtattcatcc gatgaaacac
     6481 cgccaaaagg gcctgtttga cataagcaaa atcatttact cttgtccctt cactcattta
     6541 atatcaccct taaacatctg ttcatgtatc agtttcggag tgcaaatggt cataatactg
     6601 atattcttat cgctgttgtt aacggtcctg cttcgttttc gcttcataaa taacagtctc
     6661 tttttcagag ttctgagaag accattcccg gctggcttta ttttgttttt actgtcatcc
     6721 atcatacatc tgatgataca aaaagattat tcacctttca gcggaaacat atactctgta
     6781 attatcaggt cagggatatc tgtaattaca ctatggtttt ttctgttctt tttctcatcg
     6841 tccatgctca gaacgcctgt tacgggtctt tatcttaccc cctttatatc cagcctgaga
     6901 atcgatatac atccacaata tattaccaga ccatctgtca aagtatcctc aagaatcaac
     6961 gcagacaagc ttgtcggaaa tattgaacat ttcatattat gctatccgtc ttataacacg
     7021 atcacattac agtctcacat cctgcgccac ggaatccgaa acagaatagc taataacctg
     7081 aaaaaaaaag gtatactata taaagaaaag gcaaggaaga cgccactctg tgaaactatt
     7141 gtcctgaatc tgaggtatgg gggaaaaacc cgctacagac tgttctcctg caggaggcat
     7201 cccaatggat tcagagtaca tcgcaatggc ggagcattca ttattcagac gcagaataat
     7261 taatctcatt ttttcaggag cagatatagc agaataaaac tgaacacaga ccagcaaagc
     7321 ccgactggat ctttagcccg caaagaaaaa gcggcaatcc cggaaaaata acagccggat
     7381 acggtaatac ataataaccc gggataccct gcactcaaca atccggataa aaccagaaat
     7441 acggggatca ccagtatacc tgtcagcatc acagatgctt ttgat
//
LOCUS       DAZFKF010000173         7457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFKF010000173 DAZFKF010000000
VERSION     DAZFKF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.175"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            69..275
                     /locus_tag="ACHKA1_004938"
                     /pseudo
     CDS             69..275
                     /locus_tag="ACHKA1_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(364..1500)
                     /gene="yibH"
                     /locus_tag="ACHKA1_004939"
     CDS             complement(364..1500)
                     /gene="yibH"
                     /locus_tag="ACHKA1_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5682475.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1503..1865)
                     /gene="yibI"
                     /locus_tag="ACHKA1_004940"
     CDS             complement(1503..1865)
                     /gene="yibI"
                     /locus_tag="ACHKA1_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO5682476.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2402..4315
                     /gene="mtlA"
                     /locus_tag="ACHKA1_004941"
     CDS             2402..4315
                     /gene="mtlA"
                     /locus_tag="ACHKA1_004941"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO5682477.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMFLAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGHK"
     gene            4545..5693
                     /gene="mtlD"
                     /locus_tag="ACHKA1_004942"
     CDS             4545..5693
                     /gene="mtlD"
                     /locus_tag="ACHKA1_004942"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO5682478.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDANAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5693..6280
                     /gene="mtlR"
                     /locus_tag="ACHKA1_004943"
     CDS             5693..6280
                     /gene="mtlR"
                     /locus_tag="ACHKA1_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO5682479.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPHFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(6292..6501)
                     /gene="yibT"
                     /locus_tag="ACHKA1_004944"
     CDS             complement(6292..6501)
                     /gene="yibT"
                     /locus_tag="ACHKA1_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO5682480.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6786..7148
                     /gene="yibL"
                     /locus_tag="ACHKA1_004945"
     CDS             6786..7148
                     /gene="yibL"
                     /locus_tag="ACHKA1_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO5682481.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVDAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTAF"
BASE COUNT         1912 a         1844 c         1859 g         1842 t
ORIGIN      
        1 aaaatagttg tatacatata ttccatgacc ctacattgtg atctatagat taaatatttt
       61 ttaaatgtga ctgtcatcct tcagataata aagccatcat cgcaatagat ggaaaagcac
      121 tcctgctctt ttttgattag agccgtcgca ggaggaacaa ctcatatcat cattgcgatc
      181 tcgacaatgt atagtctgat ccttcggcag atcaagacgg ataaaaaatc caatgagttc
      241 acactcatcc cgaacttctt aacataatgg atattaacga aaaatcatca cagccgatga
      301 aataatatgc aaaaataatt agtccttaca gggagcaaag ttccataatc tcccccttcc
      361 ccctcaatga tccaaataaa gataatgcat ccagctggtc attcttagca gcacttttcg
      421 catcaccgaa acatggctga aatggtcgga gtaaactgcc acctgggcgt aaatgccgtc
      481 gggtaaggca tcgatatcat cgttagggtc cagttcaatg gttcccagca caccgtccgt
      541 gccgggcacg accgttaatg attgcaatac cccctgcgcc tgataagaac cgcctggcac
      601 gacaggtaaa atactggtca gtttgccgtg gaacacctgc ccaggtagcg cgttaaacac
      661 cacttccgca tcatcgccag gtttcagacg taacagcgag ttttgccgaa attgggcgac
      721 aatttgccgt ttttgctcgg ggatgaaaac catcaccgga cgcaacggca aggcagctgc
      781 gtatgtgcct gggcggatca gtacctgagt gacgtagcca ttgcttggtg cgcgaatgac
      841 agtctgctca agattatatt ttgcttcagt aagttgcgct cttaagctca caatctgcga
      901 ttgctcgccg ttgaccatac tgtcgagctg actctggatc tgcgcctgct ccgccaccga
      961 gcctttcact aacgcatcct gcgcgaggaa attttgccgc gcatcgtcga tgtcacgttc
     1021 cgagaacgga ttcaccgccg cctggctgcc tttcaggtaa cgttgataat ttttaaacag
     1081 acggtcgcgc tccgctgaaa cctgggtggt gttggcctgc gcttcagtga gctgggcgcg
     1141 cagcgttttt atattatgcg tcgccgtcat caggtcagcc tgaagtctgt caactcgcgc
     1201 ctggtaacga accgggtcga gcttaaaaag cacctcgccc ttttgaataa gctgattatt
     1261 cttgtcagtg acttcagtaa caattcccgt cacctgtggc gtgataggga tcgctatcac
     1321 tgccttttgc gcggtaaaag tgtaagggtg gttgtagttc atcaacaaaa tcaaaccact
     1381 caccagaaac acgcctccca gcgccgccgt cgccagcgtc cactgattca ccggaatacg
     1441 aaagatttta aagaccgccc acgccagcgc cacgtaagtt aaaacaatca atagatccat
     1501 aattagatct ccgggaacgt ggaatagtca gtctttttct cggcggcgag ttgatgctcc
     1561 agccgggaaa ttcggtcaga aagtgcggcg atttccgggt cagtcgcttt ctcctgcgac
     1621 gcaacatgtg actgcatacc ccagccacgc tccggttgat agagcgtcgc ccagatccac
     1681 agaaacggcc agataacatg cagagtaaac aggctcaccc agcccgccgt atgaatagcg
     1741 tcggcatggg gatggttgcg ctttttggca atcagataag ggatgtcatg tatcgcgatg
     1801 attccataaa aaatcaccag aaagacgaag atcagcactc ccaacgcgaa atagtttaga
     1861 aacatatctg cctcggattc acgtttatca gtgttgtttt ttggctggca gccagaaggg
     1921 agtcaggctg atattttgac aataatccgg gttcgcgatt ctcgccataa caccaaagaa
     1981 taatttttag aggtgatgag ttgcttagtt acataacgat tgtatgacga aggcataaca
     2041 tgctgtagat cacatcaggt gaacgccgta agaaaatatc ttgtgattca gatcacaaag
     2101 attcaacaaa ccatcaaaac aaaaatgtga cattactcac atttaaatgc catttttagc
     2161 gaaaatcgcc tccttgttgc ttttttacac aagcgttttg tgatgaacgt cacgtcaatt
     2221 acctctctac cccctatatt tatgtgattg atatcacaca aaaggccgtc gactggacag
     2281 ttaaccgatt cagtgccaga tttcgcagta tctacaaggt ccggctacct ctgccgccac
     2341 attaacaaaa aacctcgggc ttccagcctg cgcgacagca aacataagaa ggggtgtttt
     2401 tatgtcatcc gatattaaga tcaaagtgca aagctttggt cgtttcctca gcaacatggt
     2461 gatgccaaat atcggcgcgt ttatcgcgtg gggtatcatc accgcgttat ttattccaac
     2521 agggtggtta ccgaacgaga cgctggcgaa gctggtcggg ccgatgatca cttatctcct
     2581 gccgctgctg atcggttata ccggtggtaa gctggtaggc ggcgaacgtg gcggcgtagt
     2641 cggtgccatc accaccatgg gcgttatcgt cggcgcagac atgccgatgt tcctcggttc
     2701 tatgattgca ggtccgctgg gcggctggtg cattaagcac ttcgaccgct gggtagacgg
     2761 taagatcaaa tccggttttg agatgctggt gaataacttc tccgcaggca tcatcgggat
     2821 gttcctcgcc attctggcat ttctcggcat tggcccgatt gttgaagccc tgtccaaaat
     2881 gctggctgcg ggcgttaact tcatggttgt ccatgacatg ctgccgctgg cgtctatctt
     2941 tgttgaaccg gcgaaaatcc tgttcctcaa caacgccatt aaccacggta tcttctcgcc
     3001 gctgggtatt cagcagtccc atgaactggg taaatccata ttcttcctga ttgaagctaa
     3061 cccaggtccg ggtatgggcg tgctgctggc gtacatgttc tttggtcgtg gtagcgctaa
     3121 acagtctgcg ggcggtgcgg caattatcca cttcctgggt ggtatccacg aaatttactt
     3181 cccgtatgtg ttgatgaatc cgcgtctgat cctggccgtt atcctcggcg gtatgactgg
     3241 cgtgttcacg ctgactatcc tgggcggtgg tctggtttct ccggcatctc cgggttctat
     3301 ccttgctgta ctggcgatga caccaaaagg tgcttatttc gctaacatcg cgggtgtgtg
     3361 tgcggcgatg gctgtctcct ttgttgtctc tgctattttg ctgaaaacca gcaaagtgaa
     3421 agaagaagat gatattgaag cagcaactcg tcgtatgcag gacatgaaag ctgagtctaa
     3481 aggtgcatct ccgctgtctg cgggcgatgt gactaacgac ctgagccacg tacgtaaaat
     3541 catcgttgcc tgtgacgccg gtatgggttc aagtgcgatg ggcgcaggcg tgctgcgtaa
     3601 gaaaattcag gatgcaggtc tgtcgcagat ttcggtcact aacagtgcga tcaacaacct
     3661 gccgccagat gtggacctcg tcatcactca ccgtgacctg accgaacgcg ctatgcgcca
     3721 ggttccgcag gcacagcata tttcgctgac caacttcctc gacagcggcc tgtacaccag
     3781 cctgaccgaa cgtctggttg ctgcccagcg ccatactgaa aacgaagtga aagtgaaaga
     3841 cagtctgaaa gacagctttg acgattccag tgctaacctg ttcaaactgg gcgcggagaa
     3901 catcttcctc ggtcgcaaag cggccaccaa agaagaagcg attcgttttg ctggcgagca
     3961 gctggtgaaa ggcggttacg ttgagccgga atacgttcag gcgatgctgg atcgtgaaaa
     4021 actgaccccg acttatctgg gtgagtctat cgcggtgcca cacggtacgg ttgaagcgaa
     4081 agatcgcgta ctgaaaacgg gcgtggtgtt ctgccagtac ccggaaggcg tgcgcttcgg
     4141 tgaagaagaa gatgacattg cccgtctggt gattggtatt gctgcccgta acaacgagca
     4201 cattcaggtt atcaccagcc tgaccaatgc actggatgat gagtccgtca tcgagcgtct
     4261 ggcacacacc accagcgtgg atgaagtgct ggaactgctg gcaggtcata agtaatccaa
     4321 tcccaccctc tccacatgga gaaggtgggg ctaattgcct gatgcgctac gcttatcagg
     4381 cctacaggat gcatcacaat ttgttgaatt tgcacgttct tgtaggccgg ataaggcgtt
     4441 tacgccgcat ccggcgcggc ccctctccac atggagaggg tttgggtgag ggaaaagcct
     4501 caccccagcc ctctcgggta aaaacattga tgaaggttaa tactatgaaa gcattacatt
     4561 ttggcgcagg taatatcggt cgtggcttta tcggtaaact gctggcagac gcgggtatac
     4621 aactgacgtt tgccgatgtc aatcaggtgg tacttgatgc cctgaatgcc cgtcatagct
     4681 atcaggttca tgtggtaggt gaaaccgagc aggtagatac cgtttccggc gtcaatgctg
     4741 tcagcagcat tggtgatgat gtcgttgatc tcattgctca ggttgattta gtcactaccg
     4801 ccgttggccc ggtagtgctg gaacgtattg ctccggctat cgccaaaggg ctggtgaaac
     4861 gtaaagaaca aggtaatgaa tccccgctga acatcatcgc ctgtgaaaac atggtacgcg
     4921 gtaccacgca gctgaaaggc catgtgatga acgccctgcc ggaagacgcc aacgcgtggg
     4981 tagaagaaca cgttggcttt gtcgattccg ccgttgaccg catcgtaccg ccttcggctt
     5041 cggcaactaa cgatccgctg gaagtgacgg tagaaacctt cagcgaatgg attgtcgata
     5101 aaacgcagtt caaaggtgca ctgccgaaca tcccaggcat ggagttaacc gacaacctga
     5161 tggcatttgt cgaacgtaaa ctcttcaccc tgaacacggg tcatgctata accgcgtacc
     5221 tcggaaaact ggccggtcat cagaccattc gtgacgctat tctcgacgag aaaatccgcg
     5281 cggtggtaaa aggtgcgatg gaagaaagtg gcgcggtact gatcaagcgt tacggctttg
     5341 acgctgacaa gcatgcggcg tacatccaga aaatcctcgg ccgttttgag aacccgtatc
     5401 tgaaagatga tgtagagcgc gtaggccgtc agccgctgcg taaactgagc gcaggcgacc
     5461 gtctgatcaa gccactgctc ggtacgctgg aatatggtct gccacataaa aacctgattg
     5521 aaggtattgc cgctgcaatg cacttccgca gtgaagatga tccgcaggct caggaactgg
     5581 cagcactgat cgctgacaaa ggtccgcagg cggcgctggc acagatttcc ggtcttgatg
     5641 ccaacagcga ggttgtatct gaggcggtaa ccgcttataa agcaatgcaa taatggtgga
     5701 ccaggcgcag gacaccctgc gcccgaataa cagattgtca gatatgcagg caacaatgga
     5761 acaaacccag gcctttgaaa accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg
     5821 aagctttctg atcaccgccg tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca
     5881 ggtattccgc aaagacgatt acgcggtgaa gtatgctgta gaaccgttac tcgacggcga
     5941 cggtccgctg ggcgatcttt ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa
     6001 ccgccaggaa tacgaagatg cggaactgct gatggcattg cgtgaagagc taaatcacga
     6061 cggcaacgag tacgcgttta ccgacgacga aatccttgga ccctttggtg aactgcactg
     6121 cgtggcggcg ttaccaccgc cgccacattt tgaaccagca gactccagtt tgtatgcaat
     6181 gcaaattcag cgctatcaac aggctgtgcg atcaacaatg gtcctttcac tgacagagct
     6241 gatttccaaa atcagcttaa aaaaagcctt tcaaaagtaa gcaacgtctg cttactgccc
     6301 ctctacctgc ttcggccgat aaagccgacg ataatactcc agacgttgta gatataacgg
     6361 cacgctttca tcgggtattc cggacggaat cgcgttacgg ggaggaagtt ttttcagata
     6421 ctcccggaac gcctggcttg atgccatgaa atctacggct ttatcgataa gaagcggaac
     6481 gttttcacct aatttgccca tgatgatatc tccgtattac cccgcgccgg gaatgcgcgg
     6541 ccgccaattt tagtttaggt tcccggtaaa gatgacatta aggaaaacgt gctgaatcct
     6601 caataatcat ccgcttctgt ttattatctt ttttctatag atttcttaat taatcaataa
     6661 attgcgtagt ttttaagcat attaatgatt acgttgcgga actattgcaa tgtcatcagt
     6721 tgtgaacaaa gcacctggtc gcgcatacta ggggctataa atttatcttt atcagaagcc
     6781 atcacatgaa agaagtcgaa aaaaacgaaa tcaaacgtct cagcgatcgt ctggacgcca
     6841 tccgccacca gcaggccgat ctgtcgctgg ttgatgccgc agacaaatat gccgagctgg
     6901 aaaaagagaa agccacgctg gaagcagaaa ttgcccgcct gcgtgaagtt cacagccaga
     6961 aactgagtaa agaagcacag aagctgatga agatgccgtt tcagcgcgca attaccaaaa
     7021 aagagcaggc tgatatgggc aagctgaaga aaagtgttcg cggactggtg gttgttcacc
     7081 caatgaccgc gctgggccgc gaaatgggcc tggaagagat gactggattc tctaagactg
     7141 cgttttaagc ccctcagacg attggtacga tcgtatcaac ccgcatctgg cttgcgttag
     7201 ctggtcaatg cggggactcc cctgtctgct cgcctaatta ctgaacaatc cactccctct
     7261 acatcaattt accgctggtt gatattctga aaatatattt ttaccgaaat agcaacctat
     7321 aaacaataaa aatatataga agttatcatt tgttaattaa caagcattta cgtctctttt
     7381 ttcggattta attgtgaaat tttaatcatc atattcataa aatcaactat ccgtaaaaat
     7441 ttttccagat atggttt
//
LOCUS       DAZFKF010000174         7456 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFKF010000174 DAZFKF010000000
VERSION     DAZFKF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.176"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..156)
                     /locus_tag="ACHKA1_004946"
     CDS             complement(<1..156)
                     /locus_tag="ACHKA1_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFO5682482.1"
                     /translation="MQKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQK"
     gene            complement(313..648)
                     /locus_tag="ACHKA1_004947"
     CDS             complement(313..648)
                     /locus_tag="ACHKA1_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682483.1"
                     /translation="MRTITTREQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESFEKIAEGKLPVTCPVCFAIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GNVSITGKK"
     gene            complement(765..1007)
                     /locus_tag="ACHKA1_004948"
     CDS             complement(765..1007)
                     /locus_tag="ACHKA1_004948"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFO5682484.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            complement(1088..2782)
                     /locus_tag="ACHKA1_004949"
     CDS             complement(1088..2782)
                     /locus_tag="ACHKA1_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO5682485.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTAREHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIEGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            complement(2809..3429)
                     /locus_tag="ACHKA1_004950"
     CDS             complement(2809..3429)
                     /locus_tag="ACHKA1_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO5682486.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAVFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            complement(3695..4360)
                     /locus_tag="ACHKA1_004951"
     CDS             complement(3695..4360)
                     /locus_tag="ACHKA1_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFO5682487.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(4501..5007)
                     /locus_tag="ACHKA1_004952"
     CDS             complement(4501..5007)
                     /locus_tag="ACHKA1_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005082529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO5682488.1"
                     /translation="MNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILK
                     KVRRTDKVCGYRRHIHAVFPGYFFLKADPERHSFTHLRRHSAFLNFVKMAGEIKTVRE
                     DIVQSLMKVYPDPALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHL
                     VSDDGALT"
     gene            5845..6717
                     /gene="repA"
                     /locus_tag="ACHKA1_004953"
     CDS             5845..6717
                     /gene="repA"
                     /locus_tag="ACHKA1_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO5682489.1"
                     /translation="MAALNQTSSTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAVYVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALF
                     AALDISEEAVASARRSRVEWENRQRKKQGLDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
BASE COUNT         1795 a         1850 c         1971 g         1840 t
ORIGIN      
        1 tttttgcgta tgggcgataa tggcagcgct ttcgcgaatt tgtccgtcga cgaacaccag
       61 agccggaggt gtggtgtcag gaacaggcgt ggcattaaat gccgtacagc cgctgagtaa
      121 tgacggcaga aggagtgtaa gtgatttttt ttgcatagaa taaaatccgg tatattacgg
      181 gttacggtat aagacaggtg gcgggtaact ggccttcaga tgaatatcat ttcatcgggt
      241 tattgataat gaacgttcat gttcgtgggg ggattataag agtgaattgt attttagaac
      301 gggagcatta ttttatttct tgcctgttat tgaaacattt ccggttatga tctccgaatg
      361 gcaggtatca ataaaatcac ttttccccga ttgtgtgtca aagtcaacgt gggtgaacct
      421 gtgtacatcc tgccatatgg cgaaacagac aggacaggta acaggtagtt tgccttcagc
      481 tattttttcg aagctttcaa ccagttgctg cgccttgttg tatctgacgt tatagccatt
      541 tgtgcaaagc gtttcatacc catgagctcc tgtaactata tgtgtggcga ttctttcgtg
      601 gactctccca ttaattaata attgttcacg cgttgtgatt gttctcatgc ctgtttctct
      661 gttggtcata acataaaata atctgcattc ctgaaaaata tcgcggttgt gaaatattta
      721 atttttatcc cccttttatg ggggataaaa agcaggctgt caggctataa ctgaacgcgg
      781 tttctggatt ttggtgctaa tgtcacatct ttccattcac agctgcttcc gggtatacgc
      841 tctttgcgtc gtattatacg ggagttctga cacacaataa ttaagccatt gtctgccgtc
      901 tgaaatggca ttatagcgat gtttatatct tctgttgcag gaatataact gactttcttt
      961 tcacatgtat tggtgtatat gacaactctg aatttttctg cggacatttt caatcctctt
     1021 ttaaaccaga atatcctgtg agggcattct ttgtgaaaaa gatgccggaa aaattccggc
     1081 acggggatta aacctgttgt ggttctgagt tatccggagc ggttaccggt tcgctgacat
     1141 ccggaacctt ttccggggct ggcatgacca gtggacgtgt cagggctgta agtccttcac
     1201 cggataaccc cacctccttc agcagggagt cgatgagcgg agcctgtgta cggtaagcca
     1261 gagcggcggt taccgcctgt tcagcgagat ttccgccact tatgtcggtt gttgctgttt
     1321 taccacgggt aacaccgcca ttcaggccat ccacctgaat gattttaatg ccttcaatgg
     1381 atttcattgg ttctgtggac ttctctatga cggcaggtaa tgcatccagc agggcaagac
     1441 ggaaccggag gctggtctgc tcatctgaaa gcacgttaat ggcatcgttc agtgcgcgct
     1501 gtgcttccgc ttctgccaga cctttctgac gggtggcctc cgccagttca ataattgctg
     1561 ctgcctggag ttcggctgct tctttttcag cctgggcgcg aaccgtcagt tcaaccgctt
     1621 tcgtttcagc ttcctgagca gcagcaatca gggcaacctg tttggtacgg tcggcctccg
     1681 cagtctggcg ggtggtttcc acatcctgct gggcctgtac ggcttcagcc agtgctttac
     1741 ttgcccgggc ttctgcctgt gactgctctt ccgattttgc cgccacggca atggctctgg
     1801 cctggctggc gatttcggtt gcctgctgtt gctcaatttc ccgtatggcc acttcccgtt
     1861 cggcttcgac ctggcgggta cgcactatct gctgtcgttc gatttcggct tcctctatct
     1921 tacgttcagc cagaattcgc gcggattctg cttcacgacg tcgttcagct tcaatagcag
     1981 caatactggc actctgctct gccgtacggg tttttacccg ttgttgttgc tccagagtca
     2041 tgaaggcttc ctgctgttca atctccagac gtcgggagag tgcatcccgg tttttttccc
     2101 tgatggccac ttcaacatcc tgctcaactt cgttacgttc gcggcgacga cgttctgttt
     2161 cctgggtcag aagggtcagg ccttcagcat caaaggcatt gtcaggatta aaatgttccc
     2221 gtgcagtctg gttgaaactt gtcagcgaca cactttcaag ctccagaccg tttttggata
     2281 agtcttccgc aacggtgttc tgcacaccct gaacaaagtt ctcgcgggca tcctgtaaat
     2341 cctgcatgct cattctggcc gccgtggccc gcagggcatc aacaaattta tcctcgacca
     2401 gactacgcag atcttcaggt gtgagcgtgc gttgtcccag cgtctgggct gcagtggata
     2461 ttccttcggc tgatggtttt acccgaagaa aaaaggcaac ggcaacatca acacgcatgc
     2521 ggtcgcgggt aatgagactt tcagccgctg cacgactgac ttccagcttc agggtattca
     2581 tgttgacggg aattgtttcg tgaaaaatgg gcataacaat tgcaccgcca ctcattacca
     2641 ctttttggcc tcccagacct gtgcggacaa aggcctgctc tgccgatgcc cgacggtaca
     2701 ggcgggcaaa tatcagcccg ataacgaaca gggtgaggac cagcgcaata acggtaaaca
     2761 accatcccgg aagattaaac atagtgagaa cctctgtata tgtcgggatt aaagaacatc
     2821 cggccaggga ttcagttcac ccagccagcg ggaaccggaa agacggcaga caataatcac
     2881 tttgtctcct cgtctgaatt ctttcccggc ctccggctcc agcaacaggt aatgaacctg
     2941 cccgaagcgg tctgtgaagc gaccttcaca gggggaaccc gccactgcgt gataaccggt
     3001 gatggtggcc atacagccga tgaatgcctc ctcggtgagc gccgttgttt catcacgggg
     3061 taaccagggg gcaatcagtc tgccggtgcg gtgtacgaga ggacatgtca gcagcaggca
     3121 aagtggtgca aggagaatgt tcggggcagg agcctgcagc agatgaataa taagaccctg
     3181 caggagaatg ccggatatac caaaaaaaac tgccagcata cagaggacga tcagcagggg
     3241 gatccgtccg atgttcagcc agtccagcgc ctctcctgtc agaccctccg gtaagtcagg
     3301 tgtatccata gctgaagaga gggagtgtcc gcagagcagg gcgataagtt cagcgatacc
     3361 agtaagcaga acaaaagtca gggctgccag atagggtgcg ttatacgcgg taagtaacgt
     3421 cagtgtcatt tttcctcctg tggcaggtca gtcaaacaac attatgcgtg aagcttccgc
     3481 ttctttaatt gtcagaagaa gcatatcgtc agtcatcaga agacaagggg atgtctgcag
     3541 gaggggatga taacaacaga agcctcacgc cggaatgtgc tttatgacaa caggagagga
     3601 cggataataa aaaatgggac atccacatca gcaaaccgcg atttgcgaaa aaaagtcagc
     3661 ggagagtggc tgccgttgtg gctgtcccgg tatttcagtg taacaggtga ttgctgtgat
     3721 ctatgcccca ttcggctttc ggtaaacctg aatgccccac atgaagaatg cgtgacagca
     3781 tgtttctgcc gctgccgttc aggctgcatt cgcgtccgtg cgcgagtata aaaccttcat
     3841 cccggaatat ctcttccact tctgaagatt cgagggatgc cggatgaatt aatgaaaatg
     3901 ccaccggtct gagcattctg cccgtggtgg aaaaggtgtc tctggtactg ttcagctgca
     3961 ggatatgacg tttacctgtt tgcgcacggg aaagtgtctc actcagcagc tggtcgttcc
     4021 atgcgcacgt gccaatctgc catcccagac ttctgcacgc ggaaagagcc gagcggaagc
     4081 ggtacaggtc gtataatgct gcgttattgc cactttccag cagtgatttc agggtgtttg
     4141 ctaccggtat gtctgaattg gtggccagca gttttcgcag ggtagtgaac tcttcagcca
     4201 ttcctctgaa ctcgcgcatc tgacgggcag tgtcaagcca gttcagcagg taacgccggc
     4261 gacgccgttc gttgtttatg ttgtccgggg cggtggagag tgcttcgagc atattctcca
     4321 gtaacagaat gacagggttt tcctgggata gaatggtcat aaaggcgtcc tgaaaaaatg
     4381 cccgacagcc gctctccgta caccctcagg tgtcaccggt gccgggcatt gtggaaacag
     4441 aagggaaaaa aatcccggcc ataaaggccg ggaaaagtcg tcaaattaag acagcaattt
     4501 tcatgtcaac gctccgtcat cagataccag gtgcaggagc agggcgatcc tcgactccgg
     4561 cagaggctgt gcatcaagcc ggaggaggta ctggtaacgc gcttttgtca gccagagggt
     4621 ggaggcagca tccagctcct ccctggctgc cggattgagc gcaggatccg ggtacacttt
     4681 catcaaagac tgaacgatat cctccctgac ggttttgatt tcaccggcca ttttcacgaa
     4741 gttcagaaag gcactgtgac ggcgaaggtg agtgaagcta tggcgttcgg ggtctgcttt
     4801 caggaaaaag taaccgggaa acacggcgtg tatatgtcga cggtaaccgc agactttatc
     4861 cgtgcggcgg acttttttca gtatcagggg tgtccaggga aggacgtgtt gttcggaaag
     4921 ccaggaaaag agatgttcac ggtttttgcc ggcagggatg tactgagcca gataccagtt
     4981 gcgttgatgt aattcactga tattcaaaat ccggaacgct ccgcgaaaat cgatccccgc
     5041 agaggggctt cgatgttgtt gtgaacggcg aatacataaa gcatgtgtcg gaaacagcgg
     5101 ctgaccctcg ttcagcccgg cctgcagttc tgacaggcac atgatttacg cactcttgta
     5161 agccttgtgg acggtttcga ggcgggcgaa aaccgacaac ttctgcaaac aggattaatg
     5221 gctggcaaat atattacata aggatgtatc tgtggcaaga gtgaagataa gcagttaaat
     5281 agatcgttat atttaataaa tcagcatatt atgctgattt gtgtgcggtg tctggaggat
     5341 tagatcgttt aaaaatttta ggcgatctct tctactaaaa atgatgaaaa acacagcata
     5401 aaagatcgtt tgtggaattg tctatttttt tgacatagtt caaaaaaacg acgtttttta
     5461 ggtggttaat gagaggcctg gtaaatcaga gaaatcgtgt cggcttttca aagcggtgga
     5521 aaaggggtat attgcggatc gttatgcagt ggcttttggg atcgtcagga tccgggaagt
     5581 cagaaaacgg caggatgcgc cataaggcat tcaggatgta tggcagaaac gacggcagtt
     5641 tgccggtgcc ggaaggctga aaaaagtttc agaagaccat aaaggaaaac ccccataatc
     5701 ttcttatcga acttggcgga acgtagagaa gattatgggg gcctcacaga atacgggata
     5761 agtatatatg aaaccgtacc agagattcaa cccttcgcag tgcattaacg cacggcattc
     5821 tgtttccgcc ataagcgaca gcttgtggcg gccctgaacc agacctcatc tactgaaagc
     5881 acataccgtc aggtaaagaa cccgaatccg gtcttcacac cccgtgaaga tgccgggacg
     5941 ctgaagttct gcgaaaaact gatggaaaag gcggtgggtt tcacttcccg tttcgatttc
     6001 gccgtgtatg tggcgtatgc ccgttcccgg gggctgcgtc ggcgcatgcc accggtactg
     6061 cgccgacgtg ccattgatgc gctgctgcag gggctgtgtt tccactatga cccactggcc
     6121 aaccgcgtcc agtgttccat caccacgctg gccattgagt gcggactggc gacggagtct
     6181 gctgccggaa aactctccat cacccgtgcc acccgggccc tgacgttcct gtcagagctg
     6241 gggctaatta cctaccagac ggaatatgac ccgcttattg gctgttatat tccgaccgac
     6301 atcacgttca caccggcact gtttgccgcc ctcgatattt ccgaagaggc ggtggcctcc
     6361 gcacgccgca gccgcgtgga atgggaaaac aggcagcgca aaaagcaggg gctggatgcg
     6421 ctgggtatgg atgaactgat agcgagggcc tggcgttttg tgcgcgagcg tttccgtagt
     6481 tatcaggcag agcttaagtc ccacggcatg aaacgtgccc gtgcgcgccg tgatgcaggc
     6541 agaacccggc aggacattgt caccctggtg aaacggcagc tgacgcgtga aatcgcggaa
     6601 gggcgtttcc ggggaagcct ggaggctgtg aagcgcgaaa tcgaccgtcg cgtgaaggaa
     6661 cgcatgataa tgtcgcggaa caacaactac acacggctgg ccacagcctc accctgagtt
     6721 ttacctcatc tgaataatcc ggcccgcgcc ggaggcttcc gcacgtctga aggccggcga
     6781 cgcacaaaaa agcagcgcag cacacaaaaa acagcctcat catctgcctt ccggctcgcc
     6841 ttgctccccc tgtttttcac gcaaaactcg tctcacagac gggggattct gcttatccac
     6901 atttaactgc aagggacttt tccgtaaggt tacaaccgct cattccataa agcgccagcc
     6961 gcaggcgtta cagggtgcaa tgtttctttt aaaccccagt actgtatttc ctttaaacta
     7021 cttaattagt tccatttaaa aagaaaacct attcaatgcc tgtcctgtgg acaggcaaat
     7081 atgtacctcc cactctaacg cgcgggcccc gaacctcacc gttctgaaac cacagcaaaa
     7141 aaacatcagg aataaaaaca ctacacaaac gcagcaccgt gcccacccct cataactgaa
     7201 aagcgaggcc gcccccgccc aaagggcggg aacaacatcg cttttaatta tgaatgttgt
     7261 aactaaacaa caccatcgct gtcagtcttc tggctggacg tactgaatac acgctcgtaa
     7321 gcggccctga cggcccgcta acgcggagat acgccccgac ttcgggtaaa ccctcgtcgg
     7381 gaccactccg accgcgcaca gaagctctct catggctgaa agcggatatg gcttagcagg
     7441 gtgggaatgg gatagg
//
LOCUS       DAZFKF010000175         7426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFKF010000175 DAZFKF010000000
VERSION     DAZFKF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.177"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            198..716
                     /locus_tag="ACHKA1_004954"
     CDS             198..716
                     /locus_tag="ACHKA1_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO5682490.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            926..3067
                     /locus_tag="ACHKA1_004955"
     CDS             926..3067
                     /locus_tag="ACHKA1_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO5682491.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLALSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3143..7351
                     /locus_tag="ACHKA1_004956"
     CDS             3143..7351
                     /locus_tag="ACHKA1_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
                     /protein_id="HFO5682492.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESLWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSA
                     SSSSFSSAFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGEL
                     RAVYDRSGTQVRGFAYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRF
                     EYGQDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAA
                     GRRTEYSLHMASGAVTAVTYPDGLRSSREYDEKGRLAAETSRSGETTSYSYDDPASEL
                     PTGIEDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEYDRYGQRTAVHQEEGLSIYSLY
                     DSRGRLTGQRDAQGRETRLEYSAAGDLTVIIAPDGSRSETQYDAWGKAISTTQGGLTR
                     SMEYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGRTQRYHYDLTGKLTQSEDE
                     GLITLWHYDASDRITHRTVNSDPAEQWQYDEHGWLTTLSHTSEGHRVSVHYGYDDKGR
                     LTDERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGLPPVEWLTYGSGYLAGMKL
                     GGTPLVEYTRDRLHRETVRSFGSMAGSNAAYELTSTYTLTGQLQSQHLNLPQLDRDYD
                     WNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELH
                     PDSTLTAWPDNRIAEDAHYVYRHDEYGRLAEKTDRIPEGVIRMHDERTHHYHYDSQHR
                     LVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGD
                     RLTTIQTGTTRIQTVYEPGSFTPLIRIETENGEQAKARHRSLAEVLQEDTGVTLPAEL
                     SVMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLHLYHCDHRGL
                     PQALISPEGETAWQGEYDEWGNLLGETSAQHLQQPYRLPGQQYDEESGLYYNRNRYYD
                     PLQGRYITQDPIDIKGGWNLYSYALNPVSWIDPLGLTQCDSEGCNNDILFTGGSGPDN
                     KILNELGPRDGIDGLGSQNMKMYSGLLGGDILKPGILGGLTLGSVTQRPSRTAEEEVQ
                     AQIEYLAYKKRCEQKPDSNLSRCAAAIFQKSRKEDCLRMRQEWDDKWWPGKHADEIKN
                     VKASLKNTLIDVKKRCMPTSGIPR"
BASE COUNT         1774 a         1891 c         2371 g         1390 t
ORIGIN      
        1 ttattaagtt attgctgaat aagagattta atgattttaa aaatagtttt tacgacaacc
       61 gtaaattcta gctcggtttg tatgtaaaaa atactgaaaa atgatgcggt tccgtgaagt
      121 gagctgcatc tgcctgtctg ggattaacgg caggtcattg taagtagtaa ttaaccacgt
      181 taacaaggag tttacaaatg ccaacaccgt gttatatctc catcactggt cagacccagg
      241 gaaatattac tgcaggtgca tttaccgctg attctgtcgg caacatctat gtgcaggggc
      301 acgaagacga gatgctggtg caggaattcc tgcataacgt tactgtcccg accgatcctc
      361 agtccggtca gcctgcgggt cagcgtgctc acaagccttt catttttacc gtggcgctga
      421 acaaagcggt tccgctgatg tacaacgcac tggcctccgg cgaaatgctg ccaaccactg
      481 aacttcactg gtggcgtacc tctgtggaag gcaaacagga gcattatttc actacccgcc
      541 tgactgattc gacgattgtc gatatgaaac tgcatatgcc gcattgccag gacccggcga
      601 aacgcgagtt cacccagttg ctcgaagttt cactggccta ccgcaaaatt gaatgggaac
      661 acgtcaaatc tggcacctcc ggtgccgacg actggcgcgc accgctggaa gcataagtta
      721 agccaacagc atccggctat caggccggat gctgtttttg tattacgccg tagaaagaaa
      781 ggttgtcagt aaggggttac gttcacggga gatggcggct gccggaaact tcggcggtag
      841 cgtgcctgaa cgcaagcgta acactgtact gtcacagccg ctttaacagc gaactgtctg
      901 tttttcctta accccggagg ttgctatgtc aaccggatta cgtttcacgc tggaagtgga
      961 cggcctgcca ccggatgctt ttgcggtggt ctcctttcat ctgaaccagt cactctcttc
     1021 gcttttttcc ctcgctctct ctctggtcag ccagcagttt ctctcccttg aattccagca
     1081 gatcctcgac aaaatggcct acctgacgat atggcagggc gatgacgtac agcgccgggt
     1141 gaaaggtgtg gtgacctggt ttgaactggg ggagaacgac aaaaaccaga agctgtacag
     1201 catgaaggta tgcccgccgc tgtggcgcac agggctgcgc cagaacttcc gtatcttcca
     1261 gaatgaggac atcgaaagca tcctcggcac gatattgcag gaaaacgggg tgaccgagtg
     1321 gagcccgctg ttcagcgagc cacatccttc ccgtgagttt tgtgtccagt acggtgagac
     1381 tgattacgat ttcctgtgcc ggatggcggc ggaggaaggc atcttctttt atgaggagca
     1441 cgcgcaaaaa agtaccgacc agagcctggt cctgtgcgat accgtgcgtt atctgccgga
     1501 gtcctttgag atcccctgga atccgaacac ccgtaccgag gtgagcaccc tctgcatcag
     1561 ccagtttcgc tacagcgcac aaatccgccc ttcttccgtg gtgaccaaag actacacctt
     1621 taaacgaccc ggctgggcag ggcgttttga tcaggaaggc cagtaccagg attaccagcg
     1681 cacacagtat gaagtgtatg actaccccgg acgtttcaag ggtgcccacg ggcagaactt
     1741 tgcccgctgg cagatggatg gctggcgcaa caacgcagaa gtggcgcgcg gaacaagccg
     1801 ttcgccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc aggcgaacct
     1861 gaaccgggaa tggcaggtgg tggcgagcga tcttcacggc gaacagccgc aggcggtacc
     1921 gggacgcagg ggttcaggca ccacgctgga taaccacttt gcggtaatcc cggcagaccg
     1981 gacatggcgg ccacagccgt tgctgaaacc gctggtggac ggcccgcaga gcgccgtcgt
     2041 gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc gggtgaaatt
     2101 taactgggac cgctataacc cgtcaaacca ggaaagttca tgctggatcc gtgtggcaca
     2161 ggcgtgggca ggcaccggct ttggtaacct ggcgataccg cgtgtgggtc aggaggtcat
     2221 tgtggacttc ctcaacggcg atccggacca gccgatcatt atggggcgta cctaccacca
     2281 ggaaaaccgc acccccggca gcctgccggg gacgaagacg cagatgacga tccgctcgaa
     2341 aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag ggaaagaaca
     2401 ggtctacatc cacgcgcaga agaacatgaa caccgaggtg ctgaataacc gcaccactga
     2461 tgtgataaac aaccatgccg aaaaaatagg taacaaccag gcgatcaccg ttaccaataa
     2521 ccagatccag aacattggcg ttaatcagat acagacggtt ggtgtcaacc aggtggaaac
     2581 ggtgggcagt aaccagatta tcaaagtggg atcaaaccag gttgaaaagg tggggatcat
     2641 tcgtgcgctg acggtgggtg tggcttacca gacgacggta ggcggcatta tgaatacctc
     2701 ggtggcgttg ttgcagtcct cacaggtagg gctgcataaa tcactgatgg tgggaatggg
     2761 ctacagcgtc aatgtgggga ataacgtcac cttctcggtg ggcaagacaa tgaaggaaaa
     2821 caccggacaa acagcagttt attctgccgg tgagcatctt gaactctgct gtggtaaggc
     2881 aaggctggtg ctgacgaagg acggaagcat atttctcaat ggtacgcaca ttcatctgga
     2941 aggggagtcg gatgtgaacg gtgatgcgcc agtgattaac tggaactgtg gtgccacaca
     3001 acctgtaccg gatgcgcctg tgccgaaaga tttacccccc ggaatgccgg atatgcggca
     3061 attttgagtc aggtgcaaaa aattaaaacg gaactggtgc agtgaccaga taaaccatat
     3121 taaaagcaga aggaattgat tcatgggcgg aaaaccggca gcgcggcagg gtgatatgac
     3181 ccgcaaggga ctggatattg tgcagggttc agcgggggtg ctgataggtg cgccgacggg
     3241 agtggcctgt tcggtatgtc cgggagggat tacctatgct aacccggtga acccgctgct
     3301 gggtgcgaag gtgctgccgg gcgagacgga ccttgcgctg cccggcccgc tgccgtttat
     3361 tctttcccgc gcctacagca gctaccggac cagaacaccc gcgccggtgg gggtgtttgg
     3421 tcccggctgg aaagcgccct tcgatatccg cttacagata cgcgatgaag gcctgatact
     3481 caacgacaac ggcggcagga gcattcactt tgagccgctg tttcccggcg agataagcta
     3541 cagccgcagc gagtcactct ggctggcgcg gggcggggtg gcggagcagc acagttcgca
     3601 gccgctaagc gcactctggc aggtactgcc ggaagatgtt cgcctgagtc cgcatgtgta
     3661 cctggcgaca aacagccttc aggggccgtg gtggatactc agctggccgg agcgggtgcc
     3721 gggggcggac gaggtgctgc cgccggagcc gcccgcatac cgggtgctga cgggcgtggt
     3781 ggacggcttc gggcggacgc tggcctttca ccgggcggcg gagggtgatg tggcgggcgc
     3841 ggtgacgggg gtgacggacg gcgcgggccg ccgttttcac ctggtgctga ccacacaggc
     3901 acagcgggcg gaagtattcc gtaaacagcg cgccacgtct ttatcttccc ctgccggtcc
     3961 ccgttctgct tcctcttctt ccttttcttc agctttcccc gacacgctgc ccgccggtac
     4021 agagtacggt gccgataacg gtatccggct ggaggcggtg tggctgacac acgacccggc
     4081 atacccggat gaacagccga ctgcgccgct ggcgcgctac acgtacacgg ccagcggaga
     4141 actgcgggcg gtgtatgacc gcagcgggac gcaggtgcgc gggtttgctt atgatgcgga
     4201 gcacgccggg cggatggtgg cgcaccatta tgcggggagg ccggagagcc gctaccggta
     4261 tgatgatacc ggccgggtga cggagcaggt caacccggag gggctggact accgctttga
     4321 gtacgggcag gaccgtgtga ccatcacgga cagcctgaac cggcgggagg tgctgtacac
     4381 ggagggcgag ggcggcctga agcgggtggt gaagaaggag catgcggacg ggagcattac
     4441 ccgcagcgag tatgatgagg cggggaggct gaaggcacag acggatgcgg cggggcggcg
     4501 gacggagtac agcctgcata tggcgtcggg tgcggtgacg gcggtgacgt acccggacgg
     4561 gcttcgcagc agccgggagt atgatgagaa gggaaggctg gcggcggaga cctcacgcag
     4621 cggtgagacg acgagttaca gctatgatga cccggcgagt gagctgccga cagggataga
     4681 ggatgcgacg ggcagtacaa aacagatggc atggagccgt tacggtcagc tgctggcctt
     4741 cacggactgc tcggggtaca caacgcggta tgaatacgac cggtacggtc agaggacggc
     4801 ggttcaccag gaagaaggcc tcagtattta cagtctctat gacagccgcg gcaggctgac
     4861 agggcagagg gatgcgcagg ggcgggagac gcggctggag tacagtgccg caggcgacct
     4921 caccgttatc attgccccgg acggcagccg cagtgagaca cagtatgatg cgtggggaaa
     4981 ggccatcagc accacgcagg gcgggctgac gcgcagcatg gagtatgacg ctgccgggcg
     5041 catcaccgtg ctgaccaacg agaacggcag ccagtccacg ttccggtatg acccggtgga
     5101 caggctgact gaacagcgcg gttttgacgg ccggacgcaa cgttaccact atgacctgac
     5161 cggaaaactc acgcagagtg aagacgaggg gcttatcacc ctctggcact acgatgcgtc
     5221 ggaccgcatc acgcaccgga cggtgaacag cgacccggca gagcagtggc agtatgatga
     5281 gcacgggtgg ttaaccaccc tcagccatac cagtgaaggc caccgcgtgt cggtccacta
     5341 cggctatgac gataaaggcc gcctgacgga tgaacggcag acggtggaga acccggagac
     5401 gggggagatg ctgtgggagc atgagacggg gcatgcgtac agcgaacagg ggctggcgac
     5461 ccgtcaggag ccggacggtc tgccgccggt ggagtggctg acgtatggca gcggttatct
     5521 tgcggggatg aagctgggcg gaacaccgct ggtcgagtac acgcgggacc ggctgcaccg
     5581 tgagacggtg cgcagcttcg gcagcatggc aggcagtaat gcagcatatg aactgaccag
     5641 cacatacacc ctcacaggcc agctccagag ccagcacctg aacctcccgc agcttgaccg
     5701 tgactacgac tggaacgaca acggacagct aatccgcatc agcggcccgc aggagagccg
     5761 ggagtaccgt tacagtgaca cgggaaggct gacgggcgtc cacaccacgg cagcgaacct
     5821 ggatatcgat atcccgtatg cgacggaccc ggcaggaaac cggctgccgg acccggaact
     5881 gcacccggac agcacgctca cggcgtggcc ggataaccgc atcgcggaag atgcgcacta
     5941 tgtctaccgc cacgatgaat acggcaggct ggcggagaag acggaccgca tcccggaagg
     6001 ggtgatacgg atgcacgacg agcggaccca ccactaccac tacgacagcc agcaccgtct
     6061 ggtgttctac acgcggatac agcacggtga gccgcaggtg gagagccgct acctctatga
     6121 cccgctgggc cgccggacgg gaaaacgggt gtggcggcgg gagcgtgacc tgacggggtg
     6181 gatgtcgctg tcgcgtaaac cggaggtgac atggtacggg tgggacggtg acaggctgac
     6241 gacgatacag accggcacca cgcgtatcca gacggtatac gagccgggga gcttcacacc
     6301 gctcatcaga atcgaaacag agaatggtga acaggcgaag gcgcggcacc gtagcctggc
     6361 ggaggtgttg caggaggaca cgggtgtgac gctaccggcg gagctgtcgg tgatgctggg
     6421 gaggctggag cgggaactgc ggcagggcag cgtgagtgaa gaaagccagc agtggcttgc
     6481 gcagtgcggg ctgacggcgg aacagatggc cgcgcagctg gaggcggaat atatcccgga
     6541 gcggaaactt catctttacc actgcgacca ccggggactg ccgcaggcgc tcatcagccc
     6601 ggaaggcgaa acggcgtggc agggggagta tgacgagtgg ggaaacctgc tgggcgaaac
     6661 cagcgcgcag cacctgcaac agccgtaccg tctgccggga cagcagtatg atgaggagtc
     6721 ggggctgtat tacaaccgca accggtacta tgacccgcta caggggaggt atatcaccca
     6781 ggatccaata gatataaaag gaggatggaa tttatattct tatgcgctta atccggtaag
     6841 ttggattgac ccattaggat tgacgcagtg cgattccgaa gggtgtaata atgatatatt
     6901 atttacagga ggaagtggtc ccgataataa aatacttaat gaattaggtc cgagagatgg
     6961 cattgacggt ttgggttcac aaaacatgaa aatgtatagt gggctattag ggggcgatat
     7021 tttaaaaccg gggattctgg gcggtttgac gcttggtagt gtgacacaac gaccatcgcg
     7081 aacggctgag gaggaggtgc aagctcaaat agaatattta gcatataaga agcgttgtga
     7141 gcaaaaacct gatagtaatt taagtcgttg tgctgccgcg atctttcaaa agagcagaaa
     7201 agaagattgt ttaagaatga gacaagaatg ggatgataaa tggtggcctg gcaaacatgc
     7261 ggatgaaata aaaaatgtga aagcatcgtt gaaaaacaca cttattgatg ttaaaaaaag
     7321 atgcatgcca actagtggca ttccgagata ggtatcctta aaatataatg atgttaatta
     7381 tgtccattga ttggtgaaaa tgctataata tattttctta tcttta
//
LOCUS       DAZFKF010000176         7212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFKF010000176 DAZFKF010000000
VERSION     DAZFKF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.178"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            45..176
                     /locus_tag="ACHKA1_004957"
     ncRNA           45..176
                     /ncRNA_class="other"
                     /locus_tag="ACHKA1_004957"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(360..1037)
                     /gene="narI"
                     /locus_tag="ACHKA1_004958"
     CDS             complement(360..1037)
                     /gene="narI"
                     /locus_tag="ACHKA1_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO5682493.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(1037..1747)
                     /gene="narJ"
                     /locus_tag="ACHKA1_004959"
     CDS             complement(1037..1747)
                     /gene="narJ"
                     /locus_tag="ACHKA1_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO5682494.1"
                     /translation="MIELVIVSRLLEYPDAALWQYQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LMKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1744..3282)
                     /gene="narH"
                     /locus_tag="ACHKA1_004960"
     CDS             complement(1744..3282)
                     /gene="narH"
                     /locus_tag="ACHKA1_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO5682495.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMV
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3279..7022)
                     /locus_tag="ACHKA1_004961"
     CDS             complement(3279..7022)
                     /locus_tag="ACHKA1_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO5682496.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         1598 a         2047 c         1879 g         1688 t
ORIGIN      
        1 actttcaaaa gtccctgaac ttcccaacga atccgcaatt aaatattctg cccatgcggg
       61 gaaggatgag aagcttcgac caaggttcga ctcgagcgcc aatgagaaag cgttgccgca
      121 ggcaactacc cgaagggcga agcgcgcagc gctgagtaat ccttccccca ccaccatttc
      181 ttcctgaaat actcaaaacc tgttactccg agctaattct gcccgatggc accgcgttta
      241 ccggacctac aatatcctcc cctgtaggtc ggataagcga agcgcatccg acaactcacc
      301 ccgcaccgtg cccataaaaa aaccccgccg aagcagggtc tatgtgaact aaaattcgct
      361 tagtgacgag cgcgcaccag ctggtactta cgtgtcagat actccaccgg tacgctccag
      421 atgtgtacca gacgcgagaa cgggaacagc aggaataacg tcatccccag taccaggtgt
      481 agacggaaga taaacgccac accatcgagg tgttgagaag cgccaccgtg gaaggtcacc
      541 accgactgcg cccagccaac cagtttcatc atctcgctac cgtccatatg ttgggcggag
      601 aacggaatgg tcaacaggcc cagcgcgcac tgaataacga gcagcgacag gatcaggata
      661 tccgctccgg tagtggttgc acgcacgcgt gggctgaaca gacgacgctt aagcaacagc
      721 acgccgccaa tcagacacag cacgccgctg gcaccaccag caaacatcgc cattttctgt
      781 ttcacttcaa tcggcagcca ggcttcatac atccagtgcg gcgtcaacat accgaagaag
      841 tgaccgacaa aaatccccag aataccgata tggaacaggt tcgacgccag gttcatccct
      901 ttgcgatcca gcatctggct ggacgccgcg cgccaggtgt actgcccgta gtcataacgc
      961 agccagctac caatcaggaa gaccgccccg gctatgtacg ggtagatatc aaagaagaac
     1021 atattcagga attgcattag tgctgtcctc cggtggtgat attcagatat tgcggcgcga
     1081 cggcaccggc aaagcgacgc tgatgagcag tgattgctga atcaccgcag cctttgtcag
     1141 caaagaattt aacctgctct tcttcccaga cagcatccag cgcctgcggc gtatcgtcgc
     1201 gcgcttcgtc ggcaattttt tccgccactt tgtcgctgtc gatagcggta ttcgccagtt
     1261 tcatcagcag atcaaacagc acggcataac ggctttcacg ctgttgcaga cgcgcgctca
     1321 gcaatgccag aatcggcgcg atatctttca aaccttccac ggcttcgctt tgcggtagct
     1381 gcgccaggta ctccagatac agcggcagat ggtccggcaa ttcgcggctg tttaactgca
     1441 agccgtgctg ctcgtactgc gccagcaggt ccaccatcgc ctggccgcgg tcgcgggatt
     1501 cgccgtgcac atgttcaaac agcaacagtg acgtggcgcg gccacggtcg aacagttcgc
     1561 tgtactgcgc ctgggcatcg agcggatcca tcgtcgttaa atcgcgcagg aaaatgccca
     1621 gcgcatgggc atcctctttt ggcagatttt tcgacgcggc aatcgcctca aacatctctt
     1681 gttgatactg ccataaggca gcatccggat attcaaggag acgcgataca atcacgagtt
     1741 cgatcatggg tgcggctccg ttttgctggt cacatcgatg gcatcgatac gacggctgtt
     1801 gaacagattg aatttggtat ctgaaccgtg gcagccatca ccaaaggtaa agccgcagcc
     1861 atttttctcc gggaaggctt cccgtgccag ttcacgatga ctactcggca ccacaaagcg
     1921 atcttcgtag ttagcaatcg ccagataacg gtacatctcc tgtgcctggg cttcggtcag
     1981 accgacctct tccagcgcac gggtatccac tttcccgtca acggtttcag cacgtttgta
     2041 atgacgcatc gccagcatac gtttcagcgc gcgcagtacc ggtttggtat caccggcggt
     2101 cagcagattc gccagatact gtaccggaat acgtaggctt tcgacgtctg gcagaatgcc
     2161 gttgctaccc agctcacccg cgtctgctgc agactgaatc ggagacagag gcggcacgta
     2221 ccagaccatc ggcagtgtgc gatattctgg atgcagcggc agcgccagtt tccattccat
     2281 taccatttta taaaccggcg actgctgtgc agcttcaata acgctcagcg gaataccgtc
     2341 tttaatcgcc tgctcgatga ctttcggatc gttcggatcg aggaacacct ccagctgacg
     2401 ctggtagaga tctttctcgt tctcggtgct ggctgcacgt tcaatcgcgt cggcatcgta
     2461 cagcagcacg ccaagataac ggatacgacc gacacaggtt tctgagcaca cggtcggctg
     2521 acccgcttca atacgcggat agcagaagat gcacttctca gacttaccgc tcttccagtt
     2581 gaagtagatt tttttgtatg ggcatccggt gatgcacata cgccagccac ggcatttatc
     2641 ctggtcgatc aggacgatgc catcttcttc acgcttgtaa atcgcaccgc tcgggcaggt
     2701 cgccacacat gccgggttca ggcagtgttc gcacaggcgc ggcaaataca tcatgaaggt
     2761 gttttcgaac tggctataca tcgccttctg gatgttgtcg aagttcttgt ctttcgccag
     2821 tttgtcaaac tcaccgccca ggtcatcttc ccagttcggc cctttttcga ttttcgccat
     2881 ccgttccccg gtaatcagcg aacgcggacg ggcaatcggc tgcgatttgc tgccttccgg
     2941 cgcggtatgc aggttctgat agtcaaaatc gaacggctcg taataatcgt cgatccccgg
     3001 cagatgcggg ttagcgaaga ttttacccag cagcatggca cggttaccca tgcgcggctg
     3061 cagtttgccg ttgattttac ggatccagcc gcctttgtat ttttcctggt tttcccagtc
     3121 agtcgggaag ccctggcccg gcttggtttc cacgttgttg aaccacgcgt attccacgcc
     3181 ttcacggctg gtccagacgt ttttacaggt aactgaacag gtgtggcagc cgatgcactt
     3241 atcgagattc agcaccatgc cgacttgtga acgaattttc attttacgct ctcctgtacc
     3301 tggtcattgc cttcgccatc taaccagtca atgttcttca tcttacgcac tacaacaaac
     3361 tcatcgcggt tagaacctac ggtgccgtag tagttaaagc cgtatgccag atgggcatag
     3421 ccgccgatca tatgcgtcgg tttcggcgtg atacgggtga ccgagttatg gataccacca
     3481 cgctgttggg taatttccga accaggcagg ttaacgatac gttcctgcgc gtggtacatc
     3541 atggtcatcc ctgccggaac acgctggctg acaaccgcac gggcagtcag agcaccgttg
     3601 ctgttgaata cttcaatcca gtcgttatcg gcgataccca gatctttggc atcggcttca
     3661 ctcaaccaga ccaccggacc accgcgacct aaagtcagca tcagcaggtt gtcgctgtag
     3721 gtggagtgga taccccactt ctggtgcggc gtcaggaagt tgagcgcttt ttccgggttg
     3781 ccgttggatt tctggcctat cacttctttc accgaacggg tgtcgatcgg cggacgataa
     3841 accagcaggc tttcaccgaa atcacgcatc cactggtgat cctgatacag ttgctgacga
     3901 ccagagagcg tacgccatgg gatcagctcg tgaacgttgg tgtaaccggc gttgtaagaa
     3961 acgtgttcat cttccagacc agaccaggtc gggctggaga taattttgcg cggctgtgcc
     4021 tgaatatcgc ggaagcggat cttctcgtct tctttattca gcgccagatg cgtatggtca
     4081 cgaccggtaa actcgctcag ggcagcccag gctttcacgg ctacctgacc gttggtttcc
     4141 ggtgccagcg tcaggatcat ctctgccgca tcaattgcgg tgttcagcat cggctggcct
     4201 ttcgccggac cttccgcttt ggtgtagttg agcttacgca gcagatccat ctcgctctgg
     4261 gtgttccagg caatcccttt accgccgtta ccgattttct ccatcagcgg gccgatagag
     4321 gtaaagcgtt cgtaagttgc cggataatcg cgctctacga ccataatgtg cggtgcagtt
     4381 ttgcctggga tcaggtcgca ttcgcctttt ttccagtctt tcacatccag cggctgcgcc
     4441 agttcagcgg cagagtcgtg ctggataggc agcgtgacga tgtcggtttc tttacccaga
     4501 tggccgacgc acacttcgga gaatttcttc gcgatggctt tgtagatttc ccagtcgctt
     4561 ttcgcttccc aggccggatc gaccgccgca gacagcgggt gaataaacgg atgcatatcc
     4621 gaagtattca tgtcgtcttt ttcgtaccag gtcgccgtcg gcaaaatgat gtcggaatag
     4681 agacaggtgc tcgacagacg gaagtccagc gtaaccacca gatccagctt gccttccaga
     4741 ccattgtcct gccagtccac ttcttccggc ttcacgccgc cctgctgccc cagatcttta
     4801 ccctggatac cgtgctccgt ccccagcagg tacttgagca taaactcatg acctttaccg
     4861 gaagaaccga gcaggttaga acgccagatg aacaggttgc gcgggtggtt tttaccgttt
     4921 tctggttgtt ctgccgcaaa acggatggaa ccctctttca gggatttcac cgtatagtcc
     4981 accggattca tcccggcttt ttccgcttcg ccagcgatag tcagcgggtt agtgcctaac
     5041 tgcggtgcag acggcagcca gcccatgcgt tccgcacgga cgttaaagtc gatcaggtgt
     5101 ccggtataac gggatttatc cgccatcggt gacagcaact cttccgccgt gacggtttca
     5161 taacgccact ggctggagtg gttatagaaa taagaagtgc tgttcatgtg acgcgccgga
     5221 cgctgccagt caagggcaaa cgccagcggc tgccagccgg tttgcggacg cagtttttcc
     5281 tgacctacat agtgcgccca gccgcccccg ctctgaccga cacagccgca gaaaatcagc
     5341 atgttgatca gaccacgata gttcatatcg aggtgatacc agtggttcag ccccgcaccg
     5401 acgataatca tcgaacgacc gtgcgtttta tcagcgttat cggcaaattc acgggcgatg
     5461 cgaataatct ggctgcgaga aacgccggta atctgctcgg cccaggccgg ggtataagct
     5521 ttcacatcgt catagctggt tgcacagtta acgtcgttca ggccacgttc cagaccgtag
     5581 tttgccagcg tcaaatcata aacggtggtc accagggcgg tgctgccatc agccagttgc
     5641 aggcgtttca ccggcagttt gtgcagcagc acgttttcca gttccacttt gttgaagtgt
     5701 tccgtgccgt cgccaccaaa gtacgggaag cctacctcag cgatctcatc ctgactaccc
     5761 agcaggctca gttgcagctc ggtttcttcg ccagttttgc cgtcgcgctg ctcaagattc
     5821 catttgccct tctcgcccca gcggaagcca atagaaccgt tcggcgcaac catttcgcca
     5881 ttggtattaa aggcgacagt tttccattcc ggattgtttt cctggcccag cgcatcaacc
     5941 agatcagcag cgcgcagcat acgacctgca gcgtaataac cgtcgcgttc ttccagcatc
     6001 accagcatcg gcatgtcggt gtagcgacgc acatagtcgg tgaaatactg gcttgggttg
     6061 tcgaggtgga attcacgcag cattacgtgg cccatcgcca gcgccattgc cgcatcggtg
     6121 ccctgtttcg gtgccagcca cagatcgcac agtttggcga tttcagcgta gtctggtgtg
     6181 acggcaacag ttttggtgcc tttgtaacgc acttcagtaa agaagtgagc atccggggta
     6241 cgcgtctgcg gcacgtttga accccaggcg atgatgtagc tggagttgta ccagtcagca
     6301 gattccggta cgtcagtttg ctcgccccag gtttgcggag acgcaggagg caggtcgcag
     6361 taccagtcgt agaagcttaa gcaagtaccg ccaatcagcg agagatagcg tgcacccgat
     6421 gcgtaagaaa ccatcgacat tgccggaatt ggcgagaaac cagcaacacg gtccgggccg
     6481 tagtttttga tggtgtaaac gttagatgcg gcgatcagtt cgttcacctc ctgccaggaa
     6541 gaacgaacaa aaccaccgcg tccacgcgcc tgcttaaagc ttttcgcttt atcggcgtct
     6601 tcaatgatag aagcccatgc ctcaaccgga tcgctatgca gcgccttcgc ttcacgccac
     6661 attttcatca ggcgtttgcg catcatcggg tatttcaggc ggttggcact gtaaagatac
     6721 caggagtagc tggcaccgcg cgggcagccg cgaggttcat ggtttggcag atccggacgg
     6781 gtacgcggat agtcagtctg ctgggtttcc caggtgacca gaccgttttt gacgtagatt
     6841 ttccagctgc aggagccggt gcagtttacc ccgtgggtag agcggacgat tttgtcatgc
     6901 tgccaacgct ggcgatatcc atcctcccag tcacggttgg tattgagaag ctggccatgc
     6961 ccatcggcaa aggtttcacc cttctgtttg aagtagcgaa accggtccag gaatttactc
     7021 atcggttttc tcctgtggga gcctgtcggc cctctgataa atcgacattg ctaatgttta
     7081 agggcaaggt aacgctctga aacagcgtgg gaattgataa cgatcaagag tgatggggat
     7141 gaaaaataaa gtaaattcct aagatacccc tttaggataa ttaacagtta cagcataaca
     7201 tactgtaaat ta
//
LOCUS       DAZFKF010000177         7207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFKF010000177 DAZFKF010000000
VERSION     DAZFKF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.179"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(183..1376)
                     /gene="tyrB"
                     /locus_tag="ACHKA1_004962"
     CDS             complement(183..1376)
                     /gene="tyrB"
                     /locus_tag="ACHKA1_004962"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO5682497.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNALPHGASLYLPMEGLNSYRHAIAPLLFGADHSVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLAMLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1629..2708)
                     /gene="alr"
                     /locus_tag="ACHKA1_004963"
     CDS             complement(1629..2708)
                     /gene="alr"
                     /locus_tag="ACHKA1_004963"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO5682498.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWISERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2761..4176)
                     /gene="dnaB"
                     /locus_tag="ACHKA1_004964"
     CDS             complement(2761..4176)
                     /gene="dnaB"
                     /locus_tag="ACHKA1_004964"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO5682499.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4259..5242
                     /locus_tag="ACHKA1_004965"
     CDS             4259..5242
                     /locus_tag="ACHKA1_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO5682500.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNII
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5408..5650)
                     /gene="pspG"
                     /locus_tag="ACHKA1_004966"
     CDS             complement(5408..5650)
                     /gene="pspG"
                     /locus_tag="ACHKA1_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO5682501.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5784..6821)
                     /gene="dusA"
                     /locus_tag="ACHKA1_004967"
     CDS             complement(5784..6821)
                     /gene="dusA"
                     /locus_tag="ACHKA1_004967"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO5682502.1"
                     /translation="MYGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
BASE COUNT         1834 a         1917 c         1767 g         1689 t
ORIGIN      
        1 cacaaaaaaa caggataagg aattccagat aattgggata agcttaacaa taaaagaact
       61 aacaacgttt attatctctt acgtagaacg atggcatcaa aaaatcatct ataaagaaag
      121 agtgaaagcg acgacagttt aatttcactg caggctgggt aactccagcc tgctttcctg
      181 cattacatca ccgcagcaaa cgcctttgcc acacgttgta catttgccgt atttaacccg
      241 gcgacacaca tgcgaccgct ggcgatgaga tagacaccaa attcttcacg tagtcggtca
      301 acctgagcgg cacttaaacc ggtataactg aacatgccgc gctgattaag cagataatcg
      361 aaattgcgtt ctggcatctc tgtgctcaat accttcacca gttcctgacg cattgccaga
      421 atgcgagtac gcatctcttc tacttccgcc agccagctgg ctttcaatgc ctcgtcattc
      481 agcaccgcag ccaccacctg cgcaccaaaa ttcggcgggc tggagtagtt gcggcgaact
      541 gttgctttca attgccccag tacgcggcct gcagcttcgg catcttcaca cagaacagaa
      601 agtccgccga cgcgctcgcc gtaaagggag aaaattttcg agaacgaatt gctcaccaga
      661 gcgggtaatc cagcgctggc aatggcgcga atggcgtagg catcctcttc cataccggca
      721 ccaaatcctt gataggcaat atcaaggaat gggataagct cgcgggcttt gagaatttca
      781 atcaccgcat cccactggtc attagtgaga tcggcacccg ttgggttgtg gcaacatgga
      841 tgcagcaaca caatactgcg ggcaggtaat gttttcagca tcgccaacag gtcattaaag
      901 cgcacgccgt tagtcgcttc gtcataccag gggtaagtac ttacttcgaa tccggccccg
      961 gcgaatattg ctacgtggtt ttcccaggta ggatcgctga cccagacgcc tgattccgga
     1021 aagtagcgtt tcaggaaatc cgcgcccact ttcaatgccc ctgagccgcc aagggtttga
     1081 atggttgcta cgcgctgttg ttgcagtacc gaatggtcgg caccaaacag cagcggcgca
     1141 atggcatggc gatagctgtt aagcccttcc atcggtaaat aaagcgaagc gccatgaggc
     1201 agcgcattca ggcgcgcttc tgcatcagcc acggctttca attgtggaat aattccgtct
     1261 tcgttgtagt acagaccgat acttaaattc actttgtcgc tgcgagggtc ttctttaaaa
     1321 cgctccataa gcgtaagaat cgggtcgcca gcgtaggcgt caactttttg aaacacgcga
     1381 tggttctcca ggtttacggg caggtggtta aaacacaata aaccggaaga aggcgaagat
     1441 cgagtggatg ttcaggggcg accggcaatt agcaacagag tgagactcat gacaaacgta
     1501 catccgccag aggcacgacc tttataagag catgaaagaa tatcaactta ttgaattggt
     1561 aggattttag tggccggata aggcattcac gccgcatccg gcacagacaa tcaaatatta
     1621 cagaacgatt aatccacgta tttcatcgcg acccttgaag tcaggcgcgt aataagttcg
     1681 taagcgctta ctttcgtcat ttcagcgata cgttctacgg gcaaaccttc accccacaaa
     1741 atgaccgggt ccccggcttt atcctgtgcc tgtggaccta agtctacgca gatcatatcc
     1801 atcgcgactc gcccgacaat cggcacttcg cgaccgttca ccagcactgg cgtaccagac
     1861 ggcgcggcgc gcggataacc atcgccatag cccatcgcaa ctacaccaag acgagtatca
     1921 cgttcgctta tccaggttcc accataaccg acaggctctc cggctttatg ctcacgcacg
     1981 gcaatcaggc tggaggttag tgacatcact ggctgacagc caaaatcggc accggtggag
     2041 cgatcttcca gcggcgagac gccataaaga atgatgcccg ggcgcaccca gtcaaaatgc
     2101 gactgtggcc acagcagaat gccacccgac gcggcaatgg aacgttgacc tggtttgcct
     2161 tcgcaaaagg tattaaagat agcgagttgt ttctcggttg cgccacattt tggttcatcc
     2221 gcgcgcgcaa aatggctgac gatattcacc ggctgacgaa cgtttttgca ctgggtcagg
     2281 cgatgataaa acgcctcagc ctgttccggc cttacgccca gacggtgcat accggtatcg
     2341 agtttcatcc agacggtaac cggctcgtcc aggctagcct cttccagcgc agccagctgt
     2401 tcttcgttat gcacggcggt atgaaaatgt tgcgcagaaa tcgtcggtag atctctggca
     2461 tcaaaaaagc cttcgagtaa cagtacaggt ttggtgattc cccccgcacg cagtcgcaga
     2521 gcttcttcga gacgggctac gccaaaggcg tcagcatcgg ggagcgttcg cgcggtctca
     2581 agaagaccgt gaccataagc gttcgctttc accaccgcaa ccattttact ggcaggagcc
     2641 agttcacgaa gacgttgcag gttgtgtcgc agagcgcggc ggttaatcac aacagttgcc
     2701 gcttgcattt gtgttccttg ataagtgttt gctttaatta cctaattcat aaaataatta
     2761 ttattcgtcg tcgtactgcg gccccgcata gttgtcgaag cgcgaccatt gaccgttaaa
     2821 ggtcaggcgt accgtcccga ttgggccgtt acgttgttta ccgataataa tttccgcgat
     2881 gccttttaaa tcactgtttt cgtgatacac ctcatcacga tagataaaca tgatcaagtc
     2941 cgcatcctgc tcgatagagc cagattcacg caggtcggag ttgaccgggc gtttgtcggc
     3001 acgttgttcc agagaacggt tcaactggga cagcgccacc accggcacgt tcagttcttt
     3061 cgccagtgct ttcagcgagc gggagatttc tgcaatttcc agcgtacggt tatcggaaag
     3121 cgccggtacg cgcatcagtt gcaggtagtc gatcatgata agcccgatgc cgccgtgttc
     3181 acgggcaata cggcgtgcgc gggaacgcac ttccgttggc gtcaagccgg aggagtcatc
     3241 gatatagata ttgcgttttt cgagcaaaat acccatggtg ccggaaatgc gtgcccaatc
     3301 ttcatcatcg agctgcccgg tacggatttt agtctggtca acgcgcgaca gcgacgccag
     3361 agaacgcatc ataatttgtt ctgatggcat ctccagcgag aagataagta ccggtttatc
     3421 ctgcaacatc gccgcgtttt cgacgaggtt catcgcaaat gtcgttttac ccatcgacgg
     3481 acgcgcggcg acgatgatca aatccgacgg ctgcaagcca gcggtttttt tgttgagatc
     3541 gtcataaccg gtgtttaccc cggtaacgcc atcgtgtggc tgctgaaaca actgctcaat
     3601 acgcgccacg gtggcgtcga gcacatcggc gatgttcttc ggcccttcgt ctttgttcgc
     3661 acgactttcg gcaattttaa agacgcggga ttcagccagg tccagcagat cttcgctggt
     3721 acgcccctgc ggatcaaaac cggcttcggc aatctcattc gcaaccgaga tcatctcacg
     3781 gacaacggca cgttcacgca cgatgtccgc ataggcactg atgttcgccg cacttggcgt
     3841 attttttgac agctctgcca gataagcaaa accaccgacg ctatcgagtt gcccctggcg
     3901 ttccagcgat tccgcaaggg taatcagatc gataggacta ccgctttcct gcaaacgcgc
     3961 catttcagta aagatatgac ggtgtgggcg ggtgtaaaaa tcgtctgcta ccacacgctc
     4021 ggctacatca tcccagcgtt cgttatccag cattaaaccg cccaacaccg actgctccgc
     4081 ttcgatcgag tgcggaggca ctttcagccc ggcaacttgt ggatcgcgtt cgcggggttc
     4141 agcctgctgt ttgttgaagg gtttatttcc tgccatagtg aatggagtta ccgagataaa
     4201 gaatgggtcg aaactttacc atatgaagca gaccctgacg atacgttctg gaggacacat
     4261 ggcaacacga attgaatttc acaagcacgg tggcccggaa gtacttcaag ccgtagagtt
     4321 cactcctgcc gatccggcgg agaatgaaat ccaggtcgaa aataaagcca tcggcatcaa
     4381 ttttatcgac acatatatcc gcagcggcct ttacccgccg ccatcgctac ccagcggatt
     4441 aggcaccgaa gcagcaggca tcgtgagtaa agtcggcagt ggtgtaaagc atattaaggc
     4501 aggcgatcgt gtagtctatg cgcagtcggc gttaggcgct tacagctctg tgcataacat
     4561 tattgcggat aaagcggcga ttctgcctgc ggcaatttct tttgagcaag ctgcggcatc
     4621 cttcctgaaa ggcttaacgg tttattatct gctgcgcaaa acctatgaaa ttaaacccga
     4681 tgaacagttc ctgttccacg cagcggctgg cggcgttggc ttgattgcct gccagtgggc
     4741 aaaagccctg ggcgcgaaac ttatcggcac cgtaggaacc gcgcaaaaag cgcagagcgc
     4801 gctaaaagcg ggcgcgtggc aggttattaa ctatcgtgaa gagaatctgg tcgagcggtt
     4861 aaaagagatc accggtggta aaaaagtgcg cgtggtgtac gattccgtgg gcagagacac
     4921 ctgggaacgg tcgctggatt gcctgcaacg ccgcggctta atggtgagtt ttggcaactc
     4981 atcaggtgcg gttaccggtg tgaacttagg cattctcaat cagaaaggct cgttgtatgt
     5041 gacacgccct tccctgcaag gctatatcac cacgcgggag gaattaaccg aggccagtaa
     5101 tgaactgttc tctttgattg ccagcggtgt gattaaggtc gatgtcgccg agcagcagaa
     5161 atatccgctg aaggatgcgc agcgtgcgca tgagattctg gaaagccggg cgacgcaagg
     5221 ttccagcctg ttgattccat aaaagaaata gggcttccac ctgggaagcc ctttcttttt
     5281 atagttcggc tgtatgtagg gtacagcacg atgaatctgt tagaggcgca atagtgacag
     5341 atttgattat caattcctat tttgttctaa ggataaaacc ttaggttgtg atcatccgca
     5401 caatccctta gtaacgccag cggtcataac gctgatattt cggcactttt ggtgctttaa
     5461 tcgccttaat aacccacacc accgcaatcg ccaggagtaa ccacggcagc aacttaatca
     5521 tcaatgccag cataccgccg aggaacataa tggccgtcgc cacaaccagc gcggcgataa
     5581 tgcccagcaa cgaaacgccg gtgaccatca acatgacaaa aaagccaatc acaaaaagta
     5641 gttccagcat gatgctctcc caaatatgaa atctcttgct ggcattacaa gaatcatgcc
     5701 aaaaataatc tattgattta acagcaaaac gccccgcgaa tttgcgcagg gcgtggtgaa
     5761 tttgactact ttttggtgaa aagttaacgc ttatccgcca ccagtttgag cgcgtgttcc
     5821 agcacgttaa tgtcagcacc cgctttatgg gcattttcac ttaaataacg ccgccactgc
     5881 cgcgcgccag gaataccctg gaacaagccc agcatatgcc gggtaatatg gccgagatac
     5941 gtcccctggc tgagttcacg ctcaatgtac ggatacatgg cgcgcactac cgccaccgga
     6001 tcggcatcgg tatccgagga accaaagatc tctcggtcta ccgccgccag aatacccgga
     6061 ttctgatacg cctcgcgccc gaccatcacg ccatccatat gttgcaaatg cgctttagcc
     6121 tcttccagcg acttgatacc accgttaatc gacatcgtca gatgcggaaa gtcacgcttc
     6181 agttgataca cacgcggata atcgagcggc gggatttcac ggttttcttt cgggcttaac
     6241 cccgaaagcc aggctttacg tgcgtggatg atgaacatct cacactcgcc tttgccggaa
     6301 acggtgttga tgaaatcgca gagaaattca tagctgtcct ggtcatcgat gccaatacgc
     6361 gttttcaccg tcaccggaat cgacaccaca tcgcgcatcg ctttcacgca gtcggcaacc
     6421 agctgcgcat tacccatcag acacgcacca aacatgccgt tctgcacccg gtcagacggg
     6481 cagccgacat tcaggttgat ctcatcatat ccacgcgctt ctgccagctt cgcacactgc
     6541 gccagcgccg ccggatcgct accgccgagt tgcaacgcta ccggatgttc ttcttcactg
     6601 tacgccaggt aatcaccttt accgtgaata atcgcccctg tagtcaccat ttcggtatac
     6661 agcaacgtat tgcgggaaag cagacgcaag aaatagcggc aatgtctgtc cgtccagtcg
     6721 agcattggtg caacgctaaa ccgaccactc cagtgaacgt cagttttttc aggcattgcg
     6781 ctggtttggt tgattttttg catttcagaa ttaccgtaca ttttcaaatg tagaaattat
     6841 tttatcgata tatcatgggg ttatgttatt cagcatcact gttcaggagg ctcaatagcg
     6901 ggatactata ccataacaac aggaagcgcc tgtctcattg caaaagaaaa ttgagatcct
     6961 ctcaaggcat gaagctctca cgaagtgatg gaaataatct tattagccgt tagctttgtt
     7021 aaggccaatg ataaacaatc caggttcgac gataaataaa aatcacacat taaactccgg
     7081 tgatatctct ccctgctaat agcactgata gagaaaaaaa gaacccaata agcattgggt
     7141 tcttttatgt aatgccttcc ataccatcga agaacttcac atattatttc tccgatttaa
     7201 ccccgaa
//
LOCUS       DAZFKF010000178         7139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFKF010000178 DAZFKF010000000
VERSION     DAZFKF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.180"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(269..754)
                     /gene="sixA"
                     /locus_tag="ACHKA1_004968"
     CDS             complement(269..754)
                     /gene="sixA"
                     /locus_tag="ACHKA1_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO5682503.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVTS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGEFNWQMSPCNLKMAKA
                     I"
     gene            complement(957..3101)
                     /gene="fadJ"
                     /locus_tag="ACHKA1_004969"
     CDS             complement(957..3101)
                     /gene="fadJ"
                     /locus_tag="ACHKA1_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO5682504.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCESLVEMGARGESFWKTTATDLQ"
     gene            complement(3101..4411)
                     /gene="fadI"
                     /locus_tag="ACHKA1_004970"
     CDS             complement(3101..4411)
                     /gene="fadI"
                     /locus_tag="ACHKA1_004970"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO5682505.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SNLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE"
     gene            complement(4592..4876)
                     /locus_tag="ACHKA1_004971"
     CDS             complement(4592..4876)
                     /locus_tag="ACHKA1_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO5682506.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5248..6588
                     /gene="fadL"
                     /locus_tag="ACHKA1_004972"
     CDS             5248..6588
                     /gene="fadL"
                     /locus_tag="ACHKA1_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO5682507.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVDISGRSDLTGQRADADNIAPT
                     AWVPNLHFVAPINDQFGWGASITSNYGLATEFNDNYAAGIYGGKTDLQTINLNLSGAY
                     RLNNSWSFGLGFDAVYAKAKIERTAGSLGPLLSQQLIAQGVPQSWTNGINTPDSQIAH
                     LKGDEWGFGWNAGILYELDKDNRWGLTYRSEVKIDFEGDYKSSINPNLNNILGSMGLP
                     YGTMGATQNGSLTLNLPEMWEISGYNRVAPQWAIHYSLAYTSWSQFQELKAKGDSGQT
                     LFYKDEGFRDAYRIALGTTYYHDKNWTFRTGIAFDDSPVPADKRSISIPDQDRLWLSA
                     GATYAFNEDASIDVGASYMHGQNVKFTEGEGPAAYTFKSEGKAWLFGTNFNYAF"
     gene            6953..>7139
                     /locus_tag="ACHKA1_004973"
     CDS             6953..>7139
                     /locus_tag="ACHKA1_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682508.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEVNGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVND"
BASE COUNT         1855 a         1851 c         1646 g         1787 t
ORIGIN      
        1 tattaacaca tcacaaataa tattaaatta agacacgaaa atatcatttc ttttttatta
       61 ttaactgttc tattagtttg atgctttgta aaaatacatt acaacttagt ctcatttcgt
      121 cagaaattaa tagggaagca gaggaaaaag tgcgatgaat tctgtatcga agcgtatcaa
      181 ctccgtgaaa ggttttgagt gaatatctac tattgcaaaa agacaaatgc ccgatgtaag
      241 ctgtacatcg ggcatttttt caaacgaatc agatagcttt cgccatcttc aaattacatg
      301 ggctcatctg ccagttgaac tcgccttttc cgctttcatc aagcgttacg ctggcaatgg
      361 ccgaggtggt aaacataggc ggcgtttcgc ccggacataa ctcggcgacc agatacccaa
      421 ctaacggtaa gtgggagata accaacaccg aagtaacacc ttcattggtc aacgcctgca
      481 aataggcact gaccagacca acatcgccgc agggcgttaa ctccggcaga acttctgcac
      541 tggaaggcag gttcagacaa tcccctactt cctccagtgt ttgctcggct cgcaggaacg
      601 ggctcaccag aacgcgttcg atttccactt tttgaccttt cagccagttc gccatcaggc
      661 gagattcgtc acaaccatta gtagtcagag gacgaaccga atcactggcg gcatcgaggg
      721 ctgcgtcgcc gtgacgcatg ataaaaactt gcatattgca ccgcttttgt taaccagatt
      781 cgcccgtttt ctttcatctg aaaccagaga aagaaaacgg tggccggcat tgtgccttat
      841 ccattcaccg aatgaaacgc tgttttttac ctcaatggcg taagtatagt caatatgcgt
      901 ttacattttg accaacaatc cgccattcag cacggattca tatagctttg accttcttat
      961 tgcaggtcag ttgcagttgt tttccaaaaa ctttccccac gcgcacccat ctcgactaaa
     1021 ctctcgcaag gggtaaaacg tgaaccatac tgcgtggcca gtcgttgcat tattgcaacc
     1081 acttcgcccg cgccgagaga atcgatatag cggaacggtc caccgagaaa tggcggaaaa
     1141 ccaatgccaa ataccgcacc aatatccccg tcacgcacgc tacggataac ctgctcatca
     1201 acacaacgta ctgcttcatt cagcatcaac atcacacacc gttcagcaac ctgcggtgcg
     1261 gagagtcgcc cctgcccttg tgcaccaatg agcgggtaaa tggcaggatc gacctgtttt
     1321 ttgcttttac gccctttctg accataaaga tagaaacccc gaccattttt tctgcctttg
     1381 cgatcgtcgt tcaaaattga agaaacaaca tttgcaggcg cgctaaaacg ttctccataa
     1441 gcggcttcca gtacaggaat aattttagtc ccggtgtcga ttcctacctc atccaaaagt
     1501 tggattggac ctaccggaaa accaaatttc actagcgcgg catcaatgtg ctctacccgt
     1561 tcaccttcgg tcagcatgcg gatagcttca ttaatgtaag gcgctaagat gcgattgacg
     1621 taaaacccgg ctttgtcacg cacgacaatg ggcgttttgc cctgtttttt cgccagtttt
     1681 accgtagtgg cgatggtttg cgctgatgta cccgcatgag gaataatctc caccagcggc
     1741 attttttcca ccggactgaa gaaatgcagg ccgataactt gctcaggtcg cgtggcgtga
     1801 gcggcgatat caccaatcgg taaagatgac gtgttcgaag caaagatggt atgagcggcg
     1861 caattttgct caacttccgc caccatctgt tgttttaatt cgagattttc aaacaccgct
     1921 tcaataatca gatcgcgatg ggcaaagccg cgatagtccg tcgttccgga gattaatgcc
     1981 agctgtttgt cacgttcgct ggctttgaga tgacgacggc gaactttgcc ctccagctga
     2041 tcccaactgt acttcagcgc atgatttatg ccctgcgggt tgatatcttt aattctgacc
     2101 ggaagccccg ctttacaagc agtgacataa gcaataccgc cgcccatcaa gccaccacct
     2161 aaaatcccca cgctgtttaa tggcgcaggc ggcgcatcac tgccgggatc tttcttcact
     2221 tccgtactgg caaaaaagat actacgcaaa gcttgcgatt gtggcgtcat cgccagttcg
     2281 ccaaacgccc gtgcttcagc gtcatagcca ctgctggtgc cctgcgctaa tcccgtttca
     2341 acaacctcca ggatgcgttc tgtcgccgga taattgcctt gagttttgtg ttctgttttc
     2401 ttgccgacca ttttgaacag cagcgcacga cctaacggcc ccgccagaat acgctcgcgt
     2461 acaggtagag ggcgggaaga tgggcgatcc ttctttgcca gctcaacagc ggcttccagc
     2521 agaatggagt gcggaacaac gtcatccacc agccccagct ttaatgcctg tttcgcccga
     2581 agttgttttc cggtgaggat catctctaat gctgtgctga cgcctatcag acgcggtaaa
     2641 cgctgggtgc cgcctgaacc gggtaacaat ccaagttgta cttcaggcaa accgagcacc
     2701 gttttaggat cgtcagtaca aacgcgaccg tggcacgcga gcgccagctc cagcccaccg
     2761 cccaggcaag cgccatgaat agccgcgata accggaatgg gcaaagcatg aatctccgcc
     2821 atcaactgtt ggccctgccg cgccagagct tccgcttctt gcgccgtttt gcagttgccg
     2881 atcatgttga tgtcagctcc ggcgataaag ttgtctggtt tcgcggaaat aaataccacg
     2941 ccgcgcaact ctttgttttc gcggagttgc ttaataatgg cgcgcacctg cgaggcaaat
     3001 tccgccttca gggtattcat tttctcgccc ggtacgtcga tggtgatgat agcaatgttg
     3061 tccagacgaa cattaagagt aaacgctgat gccatttcca ttattccgcc tccacaacca
     3121 ttgccgcgcc aagcccaccg gcagcacagg cggtcactaa accaaatcca ccgccgcggc
     3181 ggcgaagttc atgcaatgtc tgggttatca ttcgcgcgcc ggtcgccgca aagggatgcc
     3241 cgtatgcaat cgagccacca agcacgttaa atttgctatc gtccacttca ccggtggcat
     3301 gcgcacgccc cagtacgtcg cgagcaaaac gttcgctccc cagtagctga atattcgcca
     3361 gtgtctgagc tgcaaaggct tcgtgcatat cgatcaatgt cagattgctc atcgtcaaac
     3421 cggcacgctc cagcgccagc ggtgttgacc aggctggacc gagcaacatg tcctgccaga
     3481 catcgatagc ggtaaatgcg tagctgcgca gatatcccag cggtaccagc cctaattctt
     3541 tcgcccggga ttcagtcatc aggatcaccg ccgctgcgcc atcggtcagc ggcgtactgt
     3601 tcgccgccgt taccgttccg tgtttacgat caaacgccgg acgcagcttt gcgtaatcag
     3661 caagcgagga attaccgcga atattgttgt cttccgcgag cagttgttta taaggaggga
     3721 taaaggcagt catcacctct tctttgagtt ttccgtctga ccatgcctga gcggcacgct
     3781 gatgcgaacg gtgcgctaat gcatcttgct gttctcgggt gatgccgtag gttttcgcca
     3841 tttgctctgc agtgtcgccc atccgcaagc cggtagaata ttctgctacc gcaggtggta
     3901 cgggcattaa gtcgcgcaaa cgcaggcgag agaagagttt cagacgctgg ctcattgtgc
     3961 gggctttgtt gacatcaacc agcacgcgcg ccagtttttt actgacgcca attggcaata
     4021 ccgaagagga atctgcccca ccggcaatcc ccgctcgaat agttcccgcc atcaggcttt
     4081 ctgcgacgtt tgcaaccgcc tggaaactgg tagcgcaagc gcggctgacg ctgtaagcat
     4141 cggtatgcac attcattccg gtaccgagaa caatttcacg cgcaatgttg ggggcttcag
     4201 gcatttgtac gacctgacca aagaccagtt gttcaatcac ttcagcgggg atctcgctgc
     4261 gtgccagcag ttcgcctacc accatcttcc ccaaatcaac agcaggaatg ccatgaaaag
     4321 ccgtcgcctg gcgggcaaaa ggcgtacgta aaccgctaac aatggcgata cgatcgccct
     4381 ggcgggtaac cagcggtaaa acctgaccca taacactccc ctgtaaaaaa ataaataaag
     4441 tggtctgacc tgatcatagt cttaaccatt tttttacatt tagccaagcg gagaaaaggg
     4501 aaagtgggag ctatgacaca gagagaaaag aagaagagaa aagaaaacgc ccctgccagc
     4561 aattgacagg ggcgtacgta cccgagagga attaacgcag acccagctgg aagatcagca
     4621 tttcagcttc gcaggcaaaa gtgaaatcga tatccaagcg cacaccatca gactcttcag
     4681 tgaaagtcgg ggagattttg cacggttcgg attccacgct acgggctttc tcagtcagcg
     4741 ccgccagcgt ttgttctgct tctgcgcggt ttgcaaacac gcggctgtaa gacgcggtgc
     4801 agtcggagtt gtccataatg gtgccaacat ccatacagca gcaaaccggg gtttcatcag
     4861 cactacattt actcatcgtt gatttcctct gtatgtgcac ccaaggtgcc agataaacgt
     4921 tgtggatatt ttacgcttcc ggaaagtgct gctccagttg ttaattctgc aaaatcggat
     4981 aagtgaccga aatcacactt aaaaatgatc taaaacaaaa ttcacccgaa tccatgagtg
     5041 cgccacctcc aaattttgcc agctggatcg cgtttcttag atcatatttg aaaaaagata
     5101 gaaacatact tgcaacattc cagctggtcc gacctatact ctcgccactg gtctgatttc
     5161 taagatgtac ctcagaccct acacttcgcg ctcctgttac agcacgtaac atagtttgta
     5221 taaaaataaa tcattgaggt tatggtcatg agccagaaaa ccctgtttac aaagtctgct
     5281 ctcgcagtcg cagtggcact tatctccacc caggcctggt cggcaggctt tcagttaaac
     5341 gaattttctt cctctggcct gggccgggct tattcagggg aaggcgcaat tgccgatgat
     5401 gcaggtaacg tcagccgtaa ccccgcattg attaccatgt ttgaccgccc gacattttct
     5461 gcgggtgcgg tttatattga tccggatgta gatatttctg ggcgttccga tttaacgggc
     5521 cagcgtgctg atgcagataa tattgcacca actgcgtggg tgcctaacct acatttcgta
     5581 gcacctatta acgatcagtt tggttggggg gcttctatta cctcgaacta tggtctggcc
     5641 acggaattta atgataacta cgctgctgga atctatggcg gtaaaaccga tctgcagacg
     5701 atcaacctta accttagcgg agcctaccgc cttaacaata gctggagttt tggtcttggt
     5761 tttgatgctg tatacgctaa ggcaaaaatt gagcgtactg ccggttcact cggtccattg
     5821 ctttcccaac aactgatagc gcaaggcgtg ccacaaagtt ggacaaacgg catcaataca
     5881 ccggattcgc aaattgctca tctcaagggt gatgaatggg gattcggctg gaacgccggt
     5941 attctttatg aactggataa agataaccgt tggggcctaa cctatcgttc agaagtgaaa
     6001 attgactttg aaggagatta caaaagctcc atcaacccaa atctgaacaa cattttgggt
     6061 agcatgggtt taccatatgg cacaatgggt gcaacccaaa atgggtcctt aacgctgaat
     6121 ttacctgaga tgtgggaaat ttctggctat aaccgtgtcg caccacaatg ggctatccac
     6181 tatagtctgg cgtataccag ttggagtcaa ttccaggaac tgaaagcgaa aggagactct
     6241 ggtcaaacgc tgttctataa agacgaaggt ttccgggatg cttaccgtat cgcgttaggt
     6301 acaacctact accacgataa aaactggacc ttccgtacgg gtattgcatt cgatgacagc
     6361 ccggttccgg cagataaacg ttctatctct atcccagatc aggatcgtct gtggttaagt
     6421 gcaggtgcga cttatgcatt taatgaagat gcatccattg acgttggtgc atcttatatg
     6481 cacggtcaga atgtgaagtt tactgaaggt gaagggccag cagcatatac ctttaaatct
     6541 gaaggtaaag cctggctgtt cggtactaac tttaactacg cgttctgata acgcgttcgc
     6601 ctggataaag tcacctgcta tgcaggtgac tttaactccc ccacttcacc gaaagtagtt
     6661 tcctcattgc ttgcctcctg agttttgcaa acagcctgat ggcagcttgc cctttcaaat
     6721 cagtaagtgg tatccacaga caaacctgaa gggaaacgca tattttcagg cgttctgctc
     6781 gtccttctca aagagtttac ttttctgaat ttccaggata ctcaaccccc tggctattgt
     6841 gcgctcatac actcaaatta aagataggtt ctaaataaat gagcgttttt tgatagtcta
     6901 tttcattagg taatatatat ttgtaacaaa tcaatcaaaa tggaataaaa tcatgctacc
     6961 atctatttca atcaacaata ccagtgcagc ttacccagaa tccatcaatg aaaataacaa
     7021 tgatgaagtt aatggattag tacaagagtt caaaaacctt tttaatggta aggaaggaat
     7081 aagcacctgt attaaacatc tgcttgagct tataaaaaac gccatacgag taaacgaca
//
LOCUS       DAZFKF010000179         7125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFKF010000179 DAZFKF010000000
VERSION     DAZFKF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.181"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..182
                     /locus_tag="ACHKA1_004974"
     CDS             <1..182
                     /locus_tag="ACHKA1_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682509.1"
                     /translation="KRILHTLLKKDVKYISHYKISVFNKFEIACDSDGFYIFKALIQL
                     RRFTGFKNVRLYQLH"
     gene            225..785
                     /locus_tag="ACHKA1_004975"
     CDS             225..785
                     /locus_tag="ACHKA1_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5682510.1"
                     /translation="MFSTFKKAALLAAIALPFSTMAAPTVTFQGEVTDQTCSVNINGQ
                     TNSVVLLPTVAMADFGATLADGQSAGQTPFTVSVSNCQAPTGADQAINTTFLGYDVDA
                     STGVMGNRDTSSDAAKGFGIQLMDSSTSGNPVTLAGATNVPGLTLKVGDTEASYDFGA
                     RYFVIDSAAATAGKITAVAEYTLSYL"
     gene            871..1572
                     /locus_tag="ACHKA1_004976"
     CDS             871..1572
                     /locus_tag="ACHKA1_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309306.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5682511.1"
                     /translation="MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQL
                     RNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRMEAAAGQALRLLYTGNNLPQDRES
                     VFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNR
                     IEVTNPTGYYVVIRAAELLSNGKKVPLANSVMIAPQSTTEWTLRSGISVARGALIHLV
                     TVNDYGVNVTSEHAL"
     gene            1625..4147
                     /locus_tag="ACHKA1_004977"
     CDS             1625..4147
                     /locus_tag="ACHKA1_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5682512.1"
                     /translation="MNKSVVSISAAMLVLLCQPVMGSEISPATPSDEDNYTFDPQLFR
                     GSRFSQSSLAKLTTRESVAPGNYKMDIYTNNKLSGSWNVTFKEAADGRVLPCLTPEVA
                     DAIGLKTGEDKGEKDPVCTFAKELAPGITSQTQLSQLRLDLSVPQSQLISRPRGYVPP
                     SELDTGASLAFMNYIANYYNVAYSGQNAHSQRSLWASFNGGINLGAWQYRQLSNMTWD
                     NDKGNQWNNIRSYLQRPLPAINSQLMMGQLITSGRFFSGLSYHGVSLATDERMLPDSM
                     RGYAPTIRGVATTNARVSVMQNGHEIYQTTVAPGPFEINDLYPTSYSGDLDVTVTEAN
                     GAVSRFSVPFSAVPESMRPGTSRYNVEVGKTQDSGDDSMFGDFTWQHGLTNTLTFNSG
                     SRIADGYQALMLGGVYGSSLGAFGANLTWSHARVPESEAQSGWMSQLTWSKTFQPTST
                     TVSLAGYRYSTSGYRDLADVLGERHAASNKQSWDSSQWRQQSRFDLTLSQSLANYGNL
                     FVSGSTQNYRGGKSRDTQLQLGYSNSFSHGISMNLSVGRQRMGGYKDNSDDMQTVTSL
                     SFSFPLGGNGPRVPSLSNSWTHSTDGSSQLQSSLTGMLDEAQTTNYSLNVMRDQQYKQ
                     TTLSGNMQKRFSQTTVGLNASKGQDYWQASGNVQGAMAVHGGGVTFGPYLGETFALVE
                     AKGAEGAKVYNSSQLEINDSGYALVPAVTPYRYNRISLDPQGMDGDAELVDSEKQVAP
                     VAGAAVKVVFRTRPGKALLIKSRMADGSELPMGADVLDENNTVVGIAGQGGQIYLRTE
                     QTKGHLSVRWGEGANDSCQLPFDISGKDSNSPIIRLNETCQS"
     gene            4163..5497
                     /locus_tag="ACHKA1_004978"
     CDS             4163..5497
                     /locus_tag="ACHKA1_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5682513.1"
                     /translation="MSKSTFLHILISSIILVALIQSSAWATCSNTRVGQTEDGRSALI
                     EFGKINLTDTYFAPVGSLLATTVVPSTNYTSGGASGSSVLWECDATDLPNIYFLVATN
                     GDDRVGGFHNAGGPDGLSDVYATWFAFVGLKQTMAGVTIGRYWKKVPITSYATQGTKI
                     QIRLQDIPPLHAELYRISTLPDTAGATSYCGNTNTDGDGVGYAKPSGTPYNCTQPNAY
                     IQLSGTSGIPFAHDEVGEDSADHYDFWGADNGFGYGMRSANRLYNNATCVARSATPLV
                     LLPTIAEAQLNAGMESTGNFNVRVECSNSVQSGISDTQTALGIQVSEGAYTAAQKLGI
                     INSNGGVSALVSDNYDAAEMAKGVGIYISNSAYPDTAMTLVGQPGIAKLTPGGNAAGW
                     YPVFEGATLEGATHPGYSSYSYSFIARLKKLPNQTVSAGKVRATAYILVKMQ"
     gene            5494..6183
                     /locus_tag="ACHKA1_004979"
     CDS             5494..6183
                     /locus_tag="ACHKA1_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5682514.1"
                     /translation="MKSVLLLLTVLFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTN
                     DYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAF
                     WLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLGNDGSSS
                     YIELNNPTPLNISFTSISIGATKVQQESDMMIKPFSQRRYWLASNNVSGVVAATYLDD
                     NGAQIKQQLAQ"
     gene            complement(6329..7111)
                     /locus_tag="ACHKA1_004980"
     CDS             complement(6329..7111)
                     /locus_tag="ACHKA1_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5682515.1"
                     /translation="MSFSIEDFSLDFFSLHNKVAIVTGAAGELGRGLCSALAKAGANL
                     LLVDIKEPDNKYLKHLTHEGVEVEFMTIDITKPDAACTIINRCLERFGQLDILVNNAG
                     ICNINRPIDFNRNDWDPMINLNLNAAFDMSQAALNIFVPQRKGKVINMCSVLSFHGGR
                     WSPGYAATKHALAGLTKAYADDFAEYNIQINGIAPGYYVSEMTAIIYNNPKIKELIKG
                     RIPAQRWGRAQDLMGAMVFLASAASDYVNGQLLVIDGGYSIR"
BASE COUNT         1910 a         1757 c         1663 g         1795 t
ORIGIN      
        1 acaaaagaat attacacacc ctgctaaaaa aggatgtcaa gtacattagt cactataaaa
       61 tatcagtttt caacaagttt gaaattgctt gcgatagtga tggcttttac attttcaagg
      121 cacttataca gttaaggaga tttaccggtt ttaaaaacgt cagattgtat caattgcatt
      181 aattatctcg ttctatatta actctaaatt tcaaggatat taatatgttt tctactttca
      241 aaaaagcagc tctgctggca gctatcgcat tacctttttc taccatggct gcaccgacag
      301 tcacttttca gggtgaagta actgatcaga cctgttccgt taatatcaac ggtcaaacca
      361 attcagtagt attgctgccg accgtagcca tggctgactt cggtgcaact ttagctgatg
      421 gccagagcgc aggccagacg ccgtttacag tttctgtgtc taactgccag gctccgactg
      481 gtgcagatca ggcaatcaac accactttcc tgggctacga cgttgacgct agcacgggtg
      541 ttatgggaaa ccgtgatacc agcagcgatg cggcgaaagg ctttggcatt cagttaatgg
      601 attccagcac ttctggtaac ccagtaactc tggctggcgc gactaacgta ccgggtttga
      661 ccctgaaagt tggcgatact gaagccagct acgacttcgg tgcgcgttac ttcgttatcg
      721 atagcgcagc tgccactgcc ggtaaaatta ccgctgtcgc agaatacacc ctgagctacc
      781 tctaattaat ctactggctg gcagtaaatg ccagccagta tttcttcgca caattaatag
      841 tgatttttca tggacatgga aaagataaga atgaacattt ttgcatattt actggtactt
      901 gtattttcca tgagcatgag cagcagcgcg tttgccagcg tggtaatgac cggaacccgt
      961 attattttcc ctggtgacgc aaaggaaaaa accatccagt tgcgaaatac cagcgatcag
     1021 ccctatatca ttaatatcca tgttgaggat gaacgtggtt ctgacaagaa tgtaccgttt
     1081 atgccaaccc cgcagacatt tcgcatggaa gctgccgcag gtcaggcgtt acgcctgctc
     1141 tacactggta ataatttacc gcaggatcgc gagtctgttt tctggtttag tttcagtcaa
     1201 ctaccttatc tgaataagaa tgataaaagt cagaaccagc tcatcctggc cctgactaat
     1261 cgagtcaaaa ttttctatcg tcccagctcg attgtcggta aatccagtga cgcacccaaa
     1321 aacctgactt accaggtaaa acagaaccgc attgaagtga cgaatcccac gggctattac
     1381 gtcgtgattc gcgccgctga actgcttagt aatggtaaaa aagttcccct cgcgaattcc
     1441 gtcatgattg ctcctcaaag cacaactgaa tggacactac gctctggcat cagtgtcgct
     1501 cgcggtgcgc tgattcattt agtgaccgtc aacgactatg gcgtaaatgt tacgtctgag
     1561 catgccttat aacctctttc tccttttttg agcggtatcc tttttcacgc acgagtacgt
     1621 cgagatgaat aaatccgttg tgtcaatttc tgcggcaatg ttggttttac tttgccaacc
     1681 ggtcatgggg agcgaaatct cacccgcaac accgtcagat gaagacaact acacctttga
     1741 cccgcaactc ttccgcggca gcagatttag tcagtcgtca ttagcaaaac tgacaacacg
     1801 tgagtctgtt gcaccgggca attataaaat ggatatctac accaacaata agttgtcagg
     1861 cagttggaat gtcacgttta aagaagccgc tgatggtcgc gttctgccct gcctgacgcc
     1921 tgaagtcgcg gacgcgatag gcctcaaaac aggggaagat aagggggaaa aagatcctgt
     1981 ctgtacgttt gctaaggaac tcgctcccgg catcaccagc cagacacagt tgtcacaatt
     2041 gcgcctggac ttgtcggtgc ctcagagtca attgattagt cgccctcgcg gctatgttcc
     2101 ccctagcgag ctggataccg gagcatcgct ggcgttcatg aattatattg ccaactatta
     2161 caacgttgcc tattcagggc agaatgctca tagccagcgt tcgctatggg catcatttaa
     2221 tggcggcatt aaccttggtg cctggcaata tcgtcagtta tccaacatga cctgggataa
     2281 cgacaaaggg aatcagtgga acaatattcg tagctatttg caacgcccgc tgcccgccat
     2341 aaatagtcaa ttaatgatgg ggcaacttat caccagcgga agatttttct ctggactcag
     2401 ttatcacggc gttagtctcg cgaccgatga acgtatgctg ccggactcca tgcgcggcta
     2461 tgcgccgact attcgcggcg tggctacaac aaacgccaga gtctcggtaa tgcaaaacgg
     2521 tcatgaaatt tatcagacta ccgtggctcc aggccctttc gaaataaacg acctataccc
     2581 caccagctac agcggcgatc tggatgtcac cgttacggaa gctaatggcg cagtcagtcg
     2641 tttcagtgtc cccttttcag ccgtaccaga atcgatgcgt ccaggaactt cccgttataa
     2701 cgttgaagta ggtaaaacgc aggatagcgg tgatgactcg atgtttggtg acttcacctg
     2761 gcagcacggg ctgactaata cgctaacatt taacagtggc tcgcgtattg ctgatggcta
     2821 ccaggcgctg atgcttggcg gagtctatgg cagttcgctg ggggcatttg gggcaaacct
     2881 cacttggtcc catgcgcgtg ttcccgaaag cgaagcacag agtggttgga tgtcgcaatt
     2941 aacctggagt aaaactttcc agcctacttc aaccaccgtc tccctggcag gttatcgata
     3001 ctctaccagc ggctatcgtg atctggctga tgtgctggga gagcgtcatg ctgccagtaa
     3061 taaacagtca tgggactcca gccagtggcg tcaacagtcg cgcttcgatc ttacgttaag
     3121 tcagagcctt gcgaattacg gcaacctgtt tgtgtcaggt tcaacacaga actaccgtgg
     3181 cggcaagagc cgtgatacac agcttcagtt aggttacagc aatagcttta gccacggcat
     3241 cagtatgaac ctttccgtcg gacgccaaag aatgggcggc tataaagaca attctgatga
     3301 tatgcagacg gtaacgtccc tttcattctc attcccactt ggcggcaatg gacctcgtgt
     3361 accaagtctt agcaacagct ggacccattc aactgacggt agctcgcaat tacaaagctc
     3421 gctaaccgga atgcttgatg aagcacagac caccaactac agcctgaacg tcatgcgcga
     3481 tcaacaatat aagcagacga cgcttagcgg aaacatgcaa aaacgttttt cacaaactac
     3541 cgtcggattg aacgcatcga agggccagga ttactggcag gcgtccggaa acgtacaagg
     3601 cgcgatggcc gtccatggcg gcggcgtcac tttcggacct tatctgggtg aaacgttcgc
     3661 cctggtcgaa gcaaaaggcg cagaaggtgc aaaagtctat aactccagtc agctggaaat
     3721 taatgacagt ggctatgcgc ttgttccggc agtaacgccc tatcgctaca accgtatatc
     3781 tctcgatcca caaggaatgg atggcgatgc cgaactggtc gacagtgaaa aacaggtagc
     3841 accggttgcg ggtgcggcgg tgaaagtagt cttccgtacc cgtcctggta aagcgctgct
     3901 gattaaatcc cgcatggcag atggttcgga actgccaatg ggagccgatg tgctggatga
     3961 gaataatact gttgtcggta tagccggtca gggggggcaa atttacctcc gcacagaaca
     4021 gacaaaaggc catttgtcag ttcgctgggg tgaaggcgct aacgatagct gccagttgcc
     4081 ctttgatatc agcgggaaag acagcaatag ccctatcatc cgcctgaatg aaacctgtca
     4141 gtcttgatac agagaaaacc cgatgtctaa atcgactttt ttacacattc tgattagctc
     4201 catcatattg gtggcactaa tacaatcatc cgcctgggcc acttgttcta atacacgtgt
     4261 cggacaaacg gaggacggaa gatcggcact cattgagttc ggaaaaatta acctgaccga
     4321 cacctatttt gcgccagtag gctcactact cgcgacaaca gtcgtacctt ccactaacta
     4381 cacatcaggt ggcgcgtcag gaagctccgt actgtgggaa tgtgacgcaa cagatttgcc
     4441 aaacatctat tttctggtcg caaccaatgg cgacgaccgc gtgggtggct ttcacaatgc
     4501 aggcgggcct gatggtctga gtgatgtcta tgccacctgg tttgcttttg tcggtctcaa
     4561 gcagactatg gcgggcgtga cgattggtcg ttactggaaa aaagtgccca tcactagtta
     4621 tgccactcag ggaactaaaa tccagatccg cttacaggat atccctcctc ttcatgctga
     4681 gctttaccgt attagtacgc tgcctgatac cgctggcgca accagttact gcggtaatac
     4741 caatactgat ggcgacggtg tggggtatgc taaaccctcc ggcacacctt ataactgcac
     4801 tcagcctaat gcctatattc agctttccgg taccagtggt attccatttg ctcatgatga
     4861 agtgggcgag gacagtgcgg atcactatga tttttggggt gctgataatg gttttggtta
     4921 cggaatgcgt tcggccaatc gactctacaa caatgccacc tgcgttgccc gcagcgccac
     4981 gccgttagta ttgctgccga caattgcaga agcacaactg aatgcgggca tggaaagtac
     5041 cggtaatttt aatgtccgcg tcgagtgtag taactcggtt caatcaggga ttagcgatac
     5101 tcagactgca ttaggaatcc aggtgtctga aggtgcatat acagcggcgc aaaaactggg
     5161 gattatcaat agcaacggcg gcgtcagcgc cctagtctct gataattatg acgcagcaga
     5221 gatggcaaag ggcgttggga tctacatctc aaacagtgct taccccgata cggcgatgac
     5281 gctggttggt caaccgggca tcgcgaagtt aacccccgga ggaaatgcag cggggtggta
     5341 tcctgtattt gaaggggcaa cattagaagg tgcgactcac cccggatact ccagctatag
     5401 ttactctttt atcgcccggt tgaagaaact gccaaatcag acagtcagtg cgggaaaagt
     5461 gcgggcaacg gcgtatatat tggtgaaaat gcaatgaaat ccgttttgct gctcttaacc
     5521 gtcttgttcg tacagtcagt ggaagccggc atcatgccgg cttcttcacg gattatttat
     5581 cgcgatggcg atcgggaaaa gacactgatg ctggtcaata ccaatgacta tccggtactg
     5641 gtgcaaagtt gggtagataa tggtgaaggt tccccagaag ctgcttctcc cccctttgtg
     5701 gtgctgccac cggttttccg cctggctgcg agacgaaccc aggggttgcg gattatctat
     5761 aatcatgacc cacttcccca ggatcgcgaa tctgcattct ggttaaatct ttatgaaatg
     5821 ccgccagaaa ctccccatca agatcccaca catttaacgc tggcaatgaa tacgcaactt
     5881 aaaatctttt acaggccaaa atctgtaact atgacgcccc aagaagctat gcgtaaaata
     5941 agttggcatc tgggtaacga cggcagcagt agctatattg agcttaataa cccaacacca
     6001 ctgaatatct ctttcacctc catcagcatt ggggctacaa aggtgcagca agaaagcgat
     6061 atgatgataa aacccttcag tcaacgacgg tactggttag caagtaataa cgttagtggt
     6121 gtggttgcgg cgacatatct cgatgataat ggtgcacaga taaaacaaca attagcgcaa
     6181 taatgctctg agtggtatgt aaactcatcg cgatggttca acgctaacga ataaccacta
     6241 tacccacctg aagtaccgga agtggagaaa tgacaaaatc tctccactcc attttccagt
     6301 caaaacacct caacgcagcc aaaaatcgtt aacgaattga atatccacca tcaatgacta
     6361 atagttgtcc attgacataa tctgaagcgg cactggcgag gaacaccatt gcgcccataa
     6421 gatcctgtgc ccttccccag cgttgagccg gtatacgccc ttttattaac tcttttattt
     6481 tagggttatt ataaataata gcggtcattt cactcacata atatcctggg gcgataccat
     6541 taatctgaat attatattcc gcaaaatcat cggcatacgc tttggtcagc cccgctaaag
     6601 catgtttagt cgccgcatac cctggcgacc aacgtccccc atgaaatgac aatacggaac
     6661 acatattaat aactttccct tttctttgcg ggacaaatat attcaatgcg gcctggctca
     6721 tatcgaatgc agcattcagg ttgagattaa tcatcggatc ccagtcgttt cgattgaaat
     6781 cgataggacg attaatgtta caaatccccg cgttattgac cagaatatct aactgaccga
     6841 aacgctccag acacctatta ataatggtgc atgctgcatc aggttttgtt atgtctattg
     6901 tcataaactc aacttcaacg ccttcatgag ttaaatgctt taaatattta ttatcaggct
     6961 ctttgatgtc taccaacaac agattagctc ctgctttggc cagagcagag cataagcctc
     7021 tccccagttc acccgcggcc ccagttacga tggccacctt gttatgtaaa gaaaaaaaat
     7081 ccaatgaaaa atcttcaata gaaaagctca tatcacacat tgctc
//
LOCUS       DAZFKF010000180         7082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFKF010000180 DAZFKF010000000
VERSION     DAZFKF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.182"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(100..795)
                     /gene="aqpZ"
                     /locus_tag="ACHKA1_004981"
     CDS             complement(100..795)
                     /gene="aqpZ"
                     /locus_tag="ACHKA1_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO5682516.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1221..2879
                     /locus_tag="ACHKA1_004982"
     CDS             1221..2879
                     /locus_tag="ACHKA1_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO5682517.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CADGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2876..3832)
                     /locus_tag="ACHKA1_004983"
     CDS             complement(2876..3832)
                     /locus_tag="ACHKA1_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO5682518.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3983..5098
                     /gene="macA"
                     /locus_tag="ACHKA1_004984"
     CDS             3983..5098
                     /gene="macA"
                     /locus_tag="ACHKA1_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO5682519.1"
                     /translation="MKKRKTVKKRYVIALVIAIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5095..7041
                     /gene="macB"
                     /locus_tag="ACHKA1_004985"
     CDS             5095..7041
                     /gene="macB"
                     /locus_tag="ACHKA1_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO5682520.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTVPVVNMASGWRQFASGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
BASE COUNT         1729 a         1723 c         1919 g         1711 t
ORIGIN      
        1 cagtgactat atgtggctta acacatcaag gagcgcaaca ggaagatgaa tcaatatatt
       61 taaaaaaggc ctgacattgc gccaggcctt ctgcgttaat taattacgtt tttccagcag
      121 ggtccggtaa atcagaccac cgataatgcc gccgacaatt ggcaccaccc agaagaacca
      181 cagttgttct aatgcccagc cgccctggaa gatggcaacc gcggtgctgc gcgccgggtt
      241 aacagaagtg ttagtcaccg gaatactgat taagtgaatc agggttaagg ccagaccaat
      301 agcgatcggc gcaaaacctg ccggagcgaa tttgtcggtt gcgccgtgga tcaccaacag
      361 gaaacctgca ctcaatacca gttcaactac cagcgcggaa agcatggaat aaccgcctgg
      421 tgaatgctcg ccataaccgt tagaagcaaa accgctggct gccgcgtcaa aacccgtttt
      481 accactggca attaaataca gcagcgccgc tgcaacaata ccgccgacaa cctgggcaat
      541 tacgtagcca acgacttctt ttgccggaaa acgtccgcca gcccataaac caatagtgac
      601 cgccgggtta aaatgaccac cagaaatatg accaacagcg aaggccatcg tcagaacggt
      661 caaaccgaat gccagcgcca cgccggcaaa accaatgcct aattccggga agcctgcggc
      721 cagtacagca ctaccacagc caccaaaaac aagccagaaa gtaccaaaac attcagctgc
      781 taattttctg aacatatcca cctcaattaa aaattgaccc tgtgaaaaat atggtcgttt
      841 tatagggtcg tcgtaaaaaa gtgacgacgg aaataatgcg cggctatttt aaaaacgaag
      901 gcgagtcatt caccagataa ataaatccag taaatttgat ttagggcaac agcgggttgc
      961 cccatatagt catttgtctg attgacagtg tagtgcaagc aaaagattta atcctttagg
     1021 cgtaataaaa aataatttat catgctaatt atttgatttt gttgtttttg cagacttatc
     1081 agcaagaggg agtataacgc gattattcgc tcatttttca gacatttgcc atgcttaaat
     1141 gtgatgtcat cacgtattag caaggccttt cccgttatac tgccagcgta aaggataagt
     1201 cacatatttc tggaggggat atgattcttg agcgcgttga aattgtgggt tttcgcggta
     1261 tcaaccgttt gtcgttgatg ctggaacaaa acaacgtcct gattggggag aacgcgtggg
     1321 gtaaatccag cttgctggac gccttaaccc tgctgctatc gccagaatca gatctctacc
     1381 attttgagcg cgacgatttc tggttcccgc cgggagatat caacgggcga gaacatcatc
     1441 tgcatattat tttgaccttc cgcgaatcgc tgccaggccg acatcgggtt cgccgttatc
     1501 ggccgctgga agcgtgctgg acgccatgcg ccgatggcta tcaccgtatt ttttatcgtc
     1561 tggaagggga gagtgcggaa gacggcagcg tgatgacact gcgcagtttt ctcgataaag
     1621 acggacatcc gattgatgtt gaggatatta acgatcaggc acgccatctg gtgcgtttaa
     1681 tgccggtgct gcgcttgcgt gatgcccgtt ttatgcgccg tattcgtaac ggcacggtgc
     1741 caaatgtccc caatgtggaa gtcaccgcgc gccagctcga tttcctcgcc cgtgagttgt
     1801 cctcacatcc gcaaaatctc tctgatggac agattcgtca gggactttcc gcaatggtgc
     1861 agctgcttga gcattatttc tctgagcagg gggctggaca ggcgcgatat cgtttaatgc
     1921 ggcggcgggc cagcaatgag caacgaagct ggcgctatct ggacatcatc aaccggatga
     1981 ttgaccgacc tggagggcgc tcgtatcggg ttattttgct cggcctgttt gctactttat
     2041 tgcaggcaaa aggcacattg cgactggata aagacgcccg tccattgttg ctgatcgaag
     2101 atccagaaac gcgtttacac cccattatgc tttcagttgc ctggcatctg ttgaatcttt
     2161 tgcctttaca acgtattgct accaccaact ctggcgagct gctttcgtta actccagtgg
     2221 aacatgtttg ccgcctggtg cgtgaatctt ctcgtgttgc cgcctggcgt ctggggccaa
     2281 gtggcttgag caccgaagac agccgccgca tctcttttca cattcgtttt aatcgtccgt
     2341 catcgctgtt tgctcgctgc tggttgctgg tggaagggga aacggaaacc tgggttatca
     2401 atgaactggc gcgtcagtgc ggacatcatt ttgatgccga agggatcaag gtcattgagt
     2461 ttgcccagtc cgggctaaag ccactggtta aatttgcccg ccgaatgggg attgaatggc
     2521 atgtgctggt cgatggcgat gaagcaggga agaaatatgc cgctacggta cgcagcctgt
     2581 tgaataatga tcgggaagcc gaacgagaac atttaacggc gttaccggcg ctggatatgg
     2641 aacattttat gtatcgccag ggattttccg atgtgttcca ccgcgtggcg caaatcccgg
     2701 aaaatgtacc gatgaatctg cgcaaaatta tctcgaaagc gatccatcgc tcttccaaac
     2761 ccgatcttgc cattgaagtg gcaatggagg ccggacgtcg tggtgtggac tccgtaccga
     2821 cgctgctgaa aaaaatgttc tcacgcgtgc tgtggctggc gcgcggtcgc gcggattaac
     2881 cgcgaaacat cgtggccatt tgtggctgaa tagcgtcgag catctcatag cgccgacggt
     2941 attcagcccg ttttttactg gcgatttcgg caatctcttt tcgtgctatc tgtgctggaa
     3001 ggcggtaatg gcgttcagca tcacatacgc cgccaaccga ttcccagaaa gcgttgtaat
     3061 cggcatggat cttgccttct ttatcgcgat aacgcaggct gcggtaaata tgcgtttcat
     3121 tgctgacggc aataatctgc tctacctgca aacgttgggc aaacagacag gccgcttcca
     3181 tcacgaggcg tttgggaaat agcccgtggc aggctttcgt cgcattctgg atttcctgat
     3241 gtgggatttc ccattttgcg ccttgcagtc cgccaataaa catcgttctt ttcccctgat
     3301 attcacacag ggtaaacgtg atctctgcca gaggaatacc ttcgctgttg cggaacagga
     3361 ttgtgctgtc accttcttta tccattgaga tcatcatggt cagctcaagc gtgaactgct
     3421 cgccgttttt gccttccagc ttcgccagtt gcagcccggg ggtattcaaa tataagctga
     3481 attcttccgc cgacatacac ccgcggagta acgcataatg gtaacgtaac gcctccagca
     3541 attgcttacg gctaagattc gccgcaaggt aagggcgatg cagacgtaca ggcagtcgcg
     3601 gctggcgcgt taacaataca ttgagattag gccagtggga aagttcgttc atccactcaa
     3661 cgcttaaacg cggcataatc aacgagcgca gcaaaaattt ctggcgaaaa ctacggcgat
     3721 gccagaattt acccggccga cactgtccac gtgccagact aagaaaaagt gacaggctgc
     3781 tgagagattc agatggcgta aaggtccgtt cagttagctg cgacatattc atgaaatcaa
     3841 tggttataca tgacgtcgat ttcaccattg cgtatcttaa ccaaacatca atagtgtgat
     3901 tactaacgta aattttaggg ttttgttgat atttcgttga agttaatgac ccggattggc
     3961 atatggagta ttcagaatat ttatgaaaaa gcggaaaacc gtgaagaagc gttacgttat
     4021 tgcgctggtg atagccatcg ccggactgat tacgttatgg agaattctta acgcacccgt
     4081 gccgacttat cagacactga ttgtgcgccc cggtgattta cagcaaagcg tgctggcgac
     4141 cggaaagctg gacgcgctgc gtaaggttga tgtgggcgcg caggtcagcg gtcagttgaa
     4201 aacactgtcg gtggcgattg gcgataaagt aaaaaaagac cagcttttag gggtcattga
     4261 tcctgaacag gctgaaaacc agatcaagga ggtcgaagca acgctgatgg agctacgcgc
     4321 gcagcggcag caggcggaag cggagctgaa actggcgcgg gtgacgtatt cccgtcagca
     4381 acgtctggca caaacgcagg cggtttcaca gcaggatctc gacaccgccg cgacggagat
     4441 ggctgtgaaa caggcgcaaa ttggcaccat tgacgcgcaa atcaagcgca atcaggcttc
     4501 tctcgatacg gctaaaacca atctcgatta cactcgcatc gttgccccga tggccgggga
     4561 agtcacgcaa atcaccactc tgcaaggcca gacggtgatt gccgcacaac aagcaccgaa
     4621 cattctgacg ctggcagata tgagcaccat gctggtaaaa gcgcaggttt ctgaagcgga
     4681 tgtaatccac ctgaagccgg ggcaaaaagc ctggtttacg gtacttggcg atccactgac
     4741 gcgctacgag gggcaaatca aggatgtact accgacgccg gaaaaggtta acgacgctat
     4801 tttctattac gcccgttttg aagtccccaa ccccaatggt ttactgcggc tggatatgac
     4861 tgcgcaagtg catattcagc tcaccgatgt gaaaaatgtg ctgacgatcc ctctgtcggc
     4921 gttaggcgat ccggttggcg ataatcgtta taaagtcaaa ttgttgcgta atggtgaaac
     4981 acgcgagcgt gaagtgacga ttggcgcacg taacgatacc gatgttgaga ttgtcaaagg
     5041 gctggaagcg ggcgatgaag tggtgattgg tgaggccaaa ccaggagctg cacaatgacg
     5101 cctttgctcg aattaaagga tattcgtcgc agctatcctg ccggtgatga gcaggttgag
     5161 gtgttgaagg gcatcaccct cgatatttat gctggtgaga tggtggcgat tgttggcgct
     5221 tcgggttccg gtaaatcgac cctgatgaat attctcggct gtctggataa ggccaccagc
     5281 ggcacctatc gcgtcgccgg tcaggatgtt gccacgctgg acgccgatgc gctggcgcaa
     5341 ctgcgccgcg agcatttcgg ctttattttc cagcgttacc atttgctttc gcatttaacc
     5401 gccgagcaga acgttgaagt acccgccgtc tatgctggtc ttgagcggaa acagcggttg
     5461 cttcgcgccc aggagttgct gcaacgactg gggctggaag accgtacaga gtattatccg
     5521 gcacagcttt cgggtggtca gcaacagcgc gtcagcatcg cgcgggcatt gatgaacggc
     5581 ggtcaggtaa ttcttgccga tgaaccgacc ggcgcactgg acagccattc tggcgaagag
     5641 gtgatggcga tcctgcatca gctgcgcgac cgcgggcata cagtgattat cgtcacccac
     5701 gatccgcagg tcgctgctca ggccgagcgg gtgatcgaaa ttcgcgacgg cgaaattgtg
     5761 cgcaatcctc ccgccattga aaaagtgaat gttgctggcg ggacggtacc tgttgtcaac
     5821 atggcgtctg gctggcgtca gtttgccagc ggttttaacg aggcgctgac gatggcatgg
     5881 cgggcgctgg cagcgaataa aatgcgtact ttactgacca tgctggggat tattatcggt
     5941 attgcgtcgg tggtttccat tgtcgtagta ggtgacgccg ccaaacaaat ggtgctggcg
     6001 gatattcgtt ctattggtac gaatactatt gacgtctacc ccgggaaaga ttttggcgat
     6061 gacgatccgc aatatcagca ggcgctgaag tacgacgact taatcgccat ccaaaaacaa
     6121 ccgtgggtcg cttcagccac acctgccgtc tcgcaaaacc tgcgcctgcg ttacaataat
     6181 gttgatgttg ctgccagtgc caatggcgtg agcggcgatt attttaatgt ctatggcatg
     6241 accttcagtg aaggaaacac ctttaaccag gaacagctga acggtcgtgc gcaggtcgtg
     6301 gttctcgaca gtaatactcg ccgccagctt ttcccccata aagcagatgt ggttggcgag
     6361 gtgattctgg tcggcaatat gcccgccaga gtcattggtg tggcggaaga aaaacagtcg
     6421 atgtttggta gcagtaaagt gctgcgtgtc tggctacctt acagcacgat gtccgggcga
     6481 gttatgggcc agtcgtggct gaactccatt actgtcaggg tgaaagaagg atttgacagc
     6541 gccgaggcgg aacagcaact cacgcgttta ctttcactgc gccacggtaa gaaggatttc
     6601 tttacctgga acatggacgg cgtcttgaaa accgttgaaa agaccacacg tactttacaa
     6661 ctgtttctga cgctggtggc ggtgatttcg ctggtggtgg gcggtattgg tgtaatgaat
     6721 attatgctgg tgtcagtgac cgagcggacg cgggaaattg gcattcgcat ggctgtaggt
     6781 gcgcgagcaa gcgatgtttt gcaacagttc ctgatcgaag ccgtactggt ttgcctggtc
     6841 ggtggcgcgt tgggaataac actgtcactg ttaattgctt tcaccttgca gcttttctta
     6901 cccggttggg agattggttt ttcaccgttg gcactgctgc tggcgtttct ctgctcgacg
     6961 gccaccggga ttttatttgg ctggttaccc gcacgaaatg cggcacgact ggatccagta
     7021 gatgctctgg cacgagagta atttttgaga taaaaatgcc agccgatcgg gctggcattt
     7081 tg
//
LOCUS       DAZFKF010000181         7053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFKF010000181 DAZFKF010000000
VERSION     DAZFKF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.183"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..892
                     /gene="hlyE"
                     /locus_tag="ACHKA1_004986"
     CDS             <1..892
                     /gene="hlyE"
                     /locus_tag="ACHKA1_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFO5682521.1"
                     /translation="KTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELSRFK
                     QEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNEKKA
                     SAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSSYFQ
                     SQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSFFTTL
                     SNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAKKMIN
                     TCNEYQKRHGKKTLFEVPEV"
     gene            complement(1099..1545)
                     /locus_tag="ACHKA1_004987"
     CDS             complement(1099..1545)
                     /locus_tag="ACHKA1_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO5682522.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1637..2296)
                     /locus_tag="ACHKA1_004988"
     CDS             complement(1637..2296)
                     /locus_tag="ACHKA1_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO5682523.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(2368..2661)
                     /locus_tag="ACHKA1_004989"
     CDS             complement(2368..2661)
                     /locus_tag="ACHKA1_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO5682524.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            complement(2673..2831)
                     /locus_tag="ACHKA1_004990"
     CDS             complement(2673..2831)
                     /locus_tag="ACHKA1_004990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682525.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            2902..3303
                     /gene="pliG"
                     /locus_tag="ACHKA1_004991"
     CDS             2902..3303
                     /gene="pliG"
                     /locus_tag="ACHKA1_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO5682526.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3423..3791)
                     /locus_tag="ACHKA1_004992"
     CDS             complement(3423..3791)
                     /locus_tag="ACHKA1_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO5682527.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            4311..5006
                     /gene="minC"
                     /locus_tag="ACHKA1_004993"
     CDS             4311..5006
                     /gene="minC"
                     /locus_tag="ACHKA1_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO5682528.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            5030..5842
                     /gene="minD"
                     /locus_tag="ACHKA1_004994"
     CDS             5030..5842
                     /gene="minD"
                     /locus_tag="ACHKA1_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO5682529.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5846..6112
                     /gene="minE"
                     /locus_tag="ACHKA1_004995"
     CDS             5846..6112
                     /gene="minE"
                     /locus_tag="ACHKA1_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO5682530.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6484..>7053)
                     /locus_tag="ACHKA1_004996"
     CDS             complement(6484..>7053)
                     /locus_tag="ACHKA1_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5682531.1"
                     /translation="ASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYHSNGLG
                     GHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLSY
                     NMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNSDGNFVNDLSGRRGIYQAGIKASFSST
                     LSGHLGVGYSNGAGVESPWNAVAGVNWSF"
BASE COUNT         1983 a         1696 c         1567 g         1807 t
ORIGIN      
        1 taaaacggta gaggtagtta aaaacgcaat cgaaaccgca gatggagcat tggatcttta
       61 taataaatat ctcgatcagg tcatcccctg gcagaccttt gatgaaacca taaaagagtt
      121 aagtcgcttt aaacaggagt attcacaggc agcctccgtt ttagttggcg atattaaaac
      181 cttacttatg gatagccagg ataagtattt tgaagcaacc caaacagtgt atgaatggtg
      241 tggtgttgcg acgcaattgc tcgcagcgta tattttgcta tttgatgagt acaatgagaa
      301 gaaagcatcc gcccagaaag acattctcat taaggtactg gatgacggca tcacgaagct
      361 gaatgaagcg caaaaatccc tgctggtaag ctcacaaagt ttcaacaacg cttccgggaa
      421 actgctggcg ttagatagcc agttaaccaa tgatttttca gaaaaaagca gctatttcca
      481 gtcacaggta gataaaatca ggaaggaagc gtatgccggt gccgcagccg gtgtcgtcgc
      541 cggtccattt ggattaatca tttcctattc tattgccgcg ggcgtagttg aagggaaact
      601 gattccagaa ttgaagaaca agttaaaatc tgtgcagagt ttctttacca ccctgtctaa
      661 cacggttaaa caagcgaata aagatatcga tgccgccaaa ttgaaattaa ccaccgaaat
      721 agccgccatc ggggagataa aaacggaaac tgaaacaacc agattctatg ttgattatga
      781 tgatttaatg ctttctttgc taaaagaagc ggcaaaaaaa atgattaaca cctgtaatga
      841 gtatcagaaa agacacggta aaaagacact ctttgaggta cctgaagtct gatcagcgat
      901 tattctctcc atgtactcaa ggtataaagt ttatcacatt taaaaataca gacgtttttt
      961 aaagattgag agttacctgt tgataataag gtatcgggta actctcatta tgacagagcg
     1021 ctttcaaaaa cagggctacg aaatggcgag catccagacg tatattaaga aagcccccat
     1081 caacatctat taaatttatc actgcgccca gtcaggttta tttaagatat gatcctgcca
     1141 gtcaatcact tctgattctt tcactgcgat atgacgcaca gagatacgtt caccatgcat
     1201 tgccgctttc gaaccagtaa gtagcggatg ccacgcaggt aaatctttac cttccgccag
     1261 caaacgataa gcgcaggtca ttggcagcca ttcgaatgtt ggcagatttt cacgggttaa
     1321 tttaatgcag tcgggttcaa actcgaaacg acgttcgtag ttccgacatt gacaggtttt
     1381 aatattgagc tggcgacagg cgacgttagt gaagtagatt tcgtcggtgt cttcatccat
     1441 cagtttatgc aggcaacact gaccgcaacc atcacacaac gactcccatt ccgcatcgct
     1501 catttcgtcc agggttttac tttgccagaa aggtacatcg ctcatcaggt gttccgccat
     1561 tacgttaaaa cgcaccttat aaccagtctg gcacagcgat gcaagttttg ccgccgcttt
     1621 caggcggcaa aaagtattac aaaacgcgag ttgtcaaaga atgcccatcg aaagtgactg
     1681 tcagctcatc accgctttgc aacgggccga cgccatcagg cgtgcctgtc agcacaacgt
     1741 caccggcctt gagggtaaaa aacttgctca tataagcgat cagcggaacg attttatgga
     1801 tcatgtccgc agtcgtacct tgctggcgtt gttcgccgtt tacgctcagg cccagcgttg
     1861 tattttgcgg gtcgccggta aattccgccg cgggaataaa cccggaaagc ggacaagagt
     1921 tatcaaacgc tttagccttt tcccacggct gcccggcttt cttcattttt ccctgaacat
     1981 cacgcaatgt cagatcgagc gccacgccat aaccggcaat ggctttgcgg acatgctctt
     2041 ccgtagcctg acgcagcgtc gcgccaatca acaccgccag ttcgacttca tgatgaactg
     2101 aaccgaaatc ggatgggatc gccaatggct gccgcagatc gcacagtgcc gtttctggtt
     2161 taataaacag cactggctct tcgggcactg cgctgcccat ctctttaata tgtttggcat
     2221 agttactgcc aacacagact actttactca ccggataatc cagcagcgca ccttgccagt
     2281 tgtgatgttg atacataacg ttcccctcac cttatcgtgg atatgaaaac ccgcgccgac
     2341 aaagataatg ccaccgccgg atatcggtta tttgttagtg tcgtctgttt tctgccccat
     2401 gacggaaaga tgttgcttca gcaaatcttc gggtggtggc ggtaactgca aatagtaacc
     2461 ttgctcggtt aatgcctgtt taaccttttc aatatcggca ttgaccagtt tcttccgccc
     2521 atccagcggc agaatcatcg ctaactgagg ctgaccaaaa cctttcatca gttcctcagg
     2581 aacacgcgaa aaatcgtctt ttttttcgac atataaatag gtctggtcac gcttgctgct
     2641 tcgataaatc acacaaaaca tacttttact ctttaagatg ccaggttttg gcataagcta
     2701 acatcaatga aacccgcgcc acaagccagc cagctgtaga tttctacggt ttgttgagtc
     2761 catgcccctt tataagtaat ccccccggca aaaatgccgc gacgggattt taccgaacgc
     2821 aggaaagcca ctggaattct cccgtaaggg attgttatat tataacagtt catcgtactc
     2881 attctgaaca ggagactacc aatgaaaatc aagagcatca gtaaggctgt attgctgctt
     2941 gcactgctaa cctccacgag ctttgctgcg ggtaagaatg tcaatgttga gttcagaaaa
     3001 ggccatagca gcgctcagta ctccggcgaa ataaagggat acgattacga tacatatacc
     3061 ttctatgcca aaaaaggtca gaaagtgcat gtgagtatct ctaatgaagg tgctgatact
     3121 tacctcttcg ggccaggaat cgatgattct gttgatttat ccagatattc cccagaactc
     3181 gatagtcacg gacaatactc actccctgct tctggcaaat atgagctgcg agtgctgcaa
     3241 acccgaaacg acgcccgtaa aaacaaaact aaaaaatata acgtcgatat tcaaattaag
     3301 taaatgtcaa ccgacgggga ctctccccgt cataaacgtt aacgccccac tcctctctgt
     3361 cgctcaagag gttaatgacg aatagcagac aatgccgaat agaacataat gtaaacaagg
     3421 catcactcac gacacataaa taacatcttt gtggtggttt gattgatttt tccactccgc
     3481 ttaccatcca caccaaaata acgatcatca cgacataatt tttctttaac atcgcaaaat
     3541 acaccgttag cgaatgtaaa ttcgctggga tcgtagcctt gtaatgattg taagttttca
     3601 gcggcttttt cgccgagata tttccttgtt aactctgggg aaatgccatc tgagttcaca
     3661 caaatatacg ggtcgcaaac aacgccgcac gccggagatt gaagtccgcc agcgtcatgt
     3721 ccagacgcca ttgatgagaa catcatcccc acacctgaca atccgatata aaaaatacgc
     3781 atcatattca tagcacatcc tgataatcca accgtacaac acttatgctc atttcatcat
     3841 caatccgtaa tcaaaaccgc tgacctggac gagttacgat gacctgacag aagtatcaga
     3901 cgtcttctgc ggtttctatg atagagcaat tctgtggcgg atattaagat aacgggtatc
     3961 tgttctttaa tgatgctgca aaaaatggaa agttaagaaa gcctggcatg gtgaaaaaat
     4021 acacttgcct caatactgga tatttaaaca ggtctaaaga tgctgtcacg attagggtgc
     4081 ggaaaaagga agcgtaaata agaacaatca aaagtacgac ggcaatgggt tgattgacaa
     4141 gggtattttt taagctatga atcagcgcca tttatcacag aatagacttt tactctgaat
     4201 aaatgggagg gtgacttgcc tcaatataat ccagactata acatgcctta tagtcttcgg
     4261 aacatcatcg cgcgctggcg atgattaata gctaattgag taaggccagg atgtcaaaca
     4321 cgccaatcga gcttaaaggc agtagcttca ctttatctgt ggttcatctg catgaggcag
     4381 aacctaaggt tatccatcag gcgctggaag acaaaatcgc tcaggccccc gcatttttaa
     4441 aacatgcccc cgttgtactc aacgtcagtg cactggaaga cccggtaaac tggtcagcga
     4501 tgcataaggc ggtttcggca accggtttgc gggttattgg cgtaagtggc tgcaaagatg
     4561 cgcaacttaa agccgaaatt gaaaaaatgg ggctgcctat cctgacggaa ggaaaggaaa
     4621 aagcgccacg tccagctccc gcaccgcagg ctccagcgca aaatacaacg ccggtcacaa
     4681 aaacgcgttt aatagatacc ccggtgcgtt ccggtcagcg tatttatgct ccacaatgtg
     4741 atctgattgt tacaagccac gttagcgctg gggccgaatt aattgccgat ggtaacattc
     4801 atgtctatgg catgatgcgc ggtcgtgcgc tggcaggggc aagtggtgac cgggaaacgc
     4861 aaatattttg tacgaacctg atggcggaac tggtgtccat cgcaggtgaa tactggctga
     4921 gtgatcaaat cccagcagaa ttttatggca aagcggcgcg actgcagtta gtcgaaaacg
     4981 ctttgaccgt tcaaccgtta aattgatccc tttttaacaa ggaatttcta tggcacgcat
     5041 tattgttgtt acttcgggca aagggggtgt tggtaagaca acctccagcg cggccatcgc
     5101 cactggtttg gcccagaagg gaaagaaaac tgtcgtgata gattttgata tcggcctgcg
     5161 taatctcgac ctgattatgg gttgcgaacg ccgggtcgtt tacgatttcg tcaacgtcat
     5221 tcagggcgat gcaacgctaa atcaggcgtt aattaaagat aagcgtactg aaaatctcta
     5281 tattctgccg gcatcgcaaa cacgcgataa agatgccctc acccgtgaag gggtcgctaa
     5341 agttcttgat gatctgaaag cgatggattt tgaatttatc gtttgtgact ccccggcagg
     5401 gattgaaacc ggtgcgttaa tggcactcta ttttgcagac gaagccatta ttaccaccaa
     5461 cccggaagtc tcctcagtac gcgactctga ccgtatttta ggcattctgg cgtcaaaatc
     5521 acgccgcgca gaaaatggcg aagagcctat taaagagcac ctgctgttaa cgcgctataa
     5581 cccaggccgc gtaagcagag gtgacatgct gagcatggaa gatgtgctgg agatcctgcg
     5641 catcaaactc gtcggcgtga tcccagagga tcaatcagta ttgcgcgcct ctaaccaggg
     5701 tgaaccggtc attctcgaca ttaacgccga tgcgggtaaa gcctacgcag ataccgtaga
     5761 acgtctgttg ggagaagaac gtcctttccg cttcattgaa gaagagaaga aaggcttcct
     5821 caaacgcttg ttcggaggat aagttatggc attactcgat ttctttctct cgcggaagaa
     5881 aaacacagcc aacattgcaa aagaacggct gcagattatt gttgctgaac gccgtcgcag
     5941 cgatgcagaa ccgcattatc tgccgcagtt gcgtaaagat attcttgagg tcatttgtaa
     6001 atacgtacaa attgatcctg agatggtaac cgtacagctt gagcaaaaag atggcgatat
     6061 ttctattctt gagctgaacg tgaccttgcc ggaagcagaa gagctgaaat aggcccgctg
     6121 taaaagcgca tttatcttca aggcagagtc atctctgcct tgagtttttc atccctctca
     6181 tcgacgttgt ggtaaagcgg cgagtattct tgctgatact cctcattgct atttccacct
     6241 ccctctttac accttaaggc tgttcccacg cacagaacaa aatgtttatg cctggttgag
     6301 taaataacct tattgttagt gtggatatac tgggagatga taaccccgca atttcattat
     6361 tcattgttct cccataggat gaggtttccc gccgtaactg gcgggctttt tttgccccag
     6421 aattttctct ttcaacatcc tgtaaacgaa aactgcgccg aagcgcagtt taatcgttgg
     6481 tggtcagaac gaccagttca caccagccac cgcgttccac ggggattcca caccggcacc
     6541 gttgctatac cccaccccga gatgcccgct taacgtactg ctgaatgagg ctttaatacc
     6601 tgcctggtat attccacgtc tgcccgacaa atcattgaca aaattaccgt cactattcac
     6661 tttcacccga ttatcatcga caaattcttt gcgcacagcc gccttcagcc acggctcaac
     6721 ttccataccg tttcccagac gcatgttgta actcagcgtt gcgcccagtt cacgatatat
     6781 actgcgggta tcgactgatt tcgattccat gccattggat aaatgatatt cggggttatc
     6841 agcggtgaac cccgttaacg atgcatacgg cgtcaggttc cagttaccat cggtaaatcg
     6901 catcccggtt tcaatgtgac cgcccagccc gttgctgtgg taactgccat tggcggctcc
     6961 accgctgctc attttaccgg ctacgttact ttcaaaacgg ttcagcttca cgacaccgtc
     7021 cagatagaaa ccactttcat gttcccaact ggc
//
LOCUS       DAZFKF010000182         6992 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFKF010000182 DAZFKF010000000
VERSION     DAZFKF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.184"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(9..2792)
                     /locus_tag="ACHKA1_004997"
     CDS             complement(9..2792)
                     /locus_tag="ACHKA1_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO5682532.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLMALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2849..5221)
                     /locus_tag="ACHKA1_004998"
     CDS             complement(2849..5221)
                     /locus_tag="ACHKA1_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO5682533.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSNVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDLFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDTWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5259..6944)
                     /locus_tag="ACHKA1_004999"
     CDS             complement(5259..6944)
                     /locus_tag="ACHKA1_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO5682534.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVPP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
BASE COUNT         1803 a         1602 c         1579 g         2008 t
ORIGIN      
        1 ggctgttatt atttaggcaa taatactccg gtataagtat ttaccggatg agaaagatat
       61 tgtttaacgg cagtgttaac attctctacc gtcatttgtt tcaacaattg ctcctgctca
      121 gtccatgctg caggatcgtc atattgaata agactattta caatagtgtt cgctaattgt
      181 tgaacgctac gctgttggat atcgaggctg cgctgaacgt tttgctggta ttcgttcagt
      241 tcttgctcac tgatcccttt agccagacgc ttaaccatca cttcattcgc taacgttaac
      301 agttcatcat gtcgttctgg ttgacaagta aaagccagca aatgactgat atctttggcc
      361 tgaggatcaa ccgagaggcg agaagaaacg ctgtatgccc cagatgcctg ttcacgaata
      421 ttaacgcgta gatcttttgc cagtgcgacg ttaaaagcat cgagcgccat acgcgtcgcc
      481 agattaacag gtgtccggga atcataacgc ttccactgtg aaacctgtgc cacgggttca
      541 ttttgttcct ttacagtaac cgatgcgttg tccgtcgcgc gagttaatgg tttacctgcg
      601 gctaatggcg aatcagagtg tttgattgag cctaagtaac gcgtaattaa cgccatgagt
      661 ttgtcttctg cgacattacc gacaatgaca aacgtgatat ccgctggaga tgaaaacaac
      721 tggcgatcgg cagccagcgc atctgcggca gtaaactgtg caatctgatt ttcttgcagt
      781 aatttcgtgc ggtcatcagc atagcgcgtc tcatacatct gctgggcgaa tttctccgct
      841 ggacgctggt cgagcgtttt caacgcctga atttgagcat tttgtagcga tgcccaaata
      901 ttgtcgttaa tcgtgctgtg ggtgattcgc tggttaatca actggaaacc aggttcaggg
      961 ttattagtcc gcgcgctaac ggagagcaac gtattcatgc cactgacttt actgctcatg
     1021 gttaccgagt tttccgcact ccagcgtttc aggctggaag aggagagttc gccaacgccg
     1081 cttccgctaa ctgctttatt tgccagcgcg ataagtgatt tttgctgcgc agggaaactt
     1141 aaatcgcctt tattcgatac ggcaataatt tgtagctttt gctcttcacc cgcggatttt
     1201 gccagaataa ccctggcacc attggaaagt gttaatgatg tcagattctc agccagcgtt
     1261 tctttgctgc taatttccgc ctgtgggtca gcgtctactg ttaacgataa atttctgcct
     1321 gggaagatgt aagccgccag ctttttgttg gcgtactctt tttccagcgc caggatagcc
     1381 gcaggagaca atgctttttt tgcggcaacc tcattgttta ccatttgctc ccaaaatgcg
     1441 tcctggttct ttcttaactg ctgccatttt tccgccagac tttgcacggt aatttgctgc
     1501 cacagacgtt tcgaaagttg atatgtctct tccggcgaca agaacggcgt attatttaat
     1561 gagctggatg ccaggcgact ggtcagcata cgtaaatcac gttcagcttg ctgatcaacc
     1621 gcatttttca gccaggtgag gcgggtagat ttgacatcat cgagttcttc agcagaaaaa
     1681 ccatgctgat caatggttgc caactctgcc attaatgcat tcgcagcatc ctgcatatta
     1741 tcgtctcgtg catttacacg gaaaaacagc gactgataat cgggtgcaat tttaacgctg
     1801 cgcgcagtgc cgccagaaat agtcttcaac tcgcccgact gtatacgttc ctgcagacgt
     1861 tgattgaaca gctgaactaa catgctccat tcagcttgtt cgataaagct ttgctcatcg
     1921 ttcacttgta ccattggcag gcgataatag agtgcgatgc cgttcacccg gttttcttta
     1981 tcattaatga tattaaagcg caggtggttt tcggcttttg tcggccagac gcgattttca
     2041 gctgctttgt tagccggaag cttacttaaa ttatccttta tcagcgccag cgcttcttta
     2101 ctgtcgatat cgccaaccac gataaaggtc atattatttg gttgatacca gcgttgataa
     2161 aattggcgca attgtgccgg tgtgaccgtg gcgacagtat ccatcaggcc gataggttca
     2221 cggtctaaat tacgggtatt agccagcagg aaagggcggc gcgcctgaga ggtgcgccat
     2281 ttcgcatcct gatgggcacg ccattcctca gtaattacgc cgcgttcagc gtctacttcg
     2341 agtttttcaa aggttgcggc attactccat tcactgaaga ttgccatcac ttgttgcaga
     2401 ttttgtttct gcgtagttgg caaactcacc tgatacaccg tttcgtcata gctggtatag
     2461 gcattaacat cgcgaccaaa acgcaggccc attgactcaa atgtttcgat gactttatta
     2521 cccggccatg tttttgtgcc gttaaacatc atatgttcta caaaatgagc cacgccgcgc
     2581 tcattgtctt cttcctgcaa tgaaccggta tgaatttgca gccataaatt tacctgatcc
     2641 tttggatgag catgcggata aatcatatat cgcaagccat tgtccaattg cccggtaatt
     2701 aacttttcat cctgcggtaa ggcggcggca atcagccgcc cggggagcaa cagagttgcc
     2761 actaacgtca gtaagaaaca gaggtttctc ataattatct ccatgcgaaa accgggcgaa
     2821 tttacccggt taagtaaaat ccgaactatt aaaatttcat gctgacatca agccagaaag
     2881 tacggccact ggcgtaggtc gccacaccgg tattcgttgt atcaaccgcg gttttgctat
     2941 ccagtacatt gagaatatcg gcactgatcg tcaggttttg ttgtttcaga aattgtggcg
     3001 tccaggacaa gcgggtgtcc caggtcaggc tgctatccag tttttcgtca acatactgct
     3061 tgtaatctga atattcgctg atgtattgcg cattcgtctt acccaggata atgcgagctt
     3121 tacgcgcttc ttgccaggcc agcgtattcg cccacaccaa cccgctcggt tgatgcgtga
     3181 aatccatgtt taaggagatc tttaatgggt tattaaaatc tgccactgga acgctatcgt
     3241 aagagacaag attaccgtta taaaccacct ggttatcacc ggtattgctc tcctcataac
     3301 cattgtttaa cgacaagttg cccttgctct tgatatagct aaagacaatt tgtgggttaa
     3361 tatctacctg gcggatatgc aggggttcgg ccagctcaaa actgaggttg aacgaatgcg
     3421 ttttggtttt gccatcgttg ttatattcag taatagtggt tttagtcgcg ctgtcggtac
     3481 gactgctttt gctgatttga tcatgcgctt cacggtaaac atagtttgca cgcgcaataa
     3541 cgttcttacc gattttctgc tgcaatccca ttgccagttc atcgttataa ggcgttttca
     3601 aatcctgata acgcgtcagg gttttattgc ctgataccga ttccgtccag ctattgcgga
     3661 tatcacgtaa tcccatatca agaatattcc caccatagta acggttataa cctgcggtaa
     3721 tcattgaggt ttgatcagca aaaatatccc attccgtcat aaagcgcggg gagatattgt
     3781 ggtttgacag atagttgtca tagtcatacc gcacgccggg cattaatgac acattacgcc
     3841 agctaatgcg atccgccata tacagtgtgt agttgtcaat tcccaggctg cctttacctt
     3901 tatggtaaat ggtatggtta gttttctttc cggcagcatt aatcacatag gattcagact
     3961 ggttatggcg ttcagtccat gcatcggaat agatgtattc cgcgccgaag tagggttgat
     4021 gcgaaacatt acccacggcg aatttttgcc agtccaggcg tgttttgaag gtgtaattat
     4081 ctacagcctg ggaaatgtgt cctaatccgc cacgggtgca acgccctgta atatcaccat
     4141 atgtacatga aagttcggtg taccaggtgt catgatcgtg acgggtataa tcactaatat
     4201 gatcccaacc aacggtggta cgtagtttgg cccatgcgag ctgcgtatcc atatcccatg
     4261 ccagaccata ggatttatta cccatttcgc gatcagactg cgggaaggtg ctggtattat
     4321 aatcacggct ggagccggta tattttaagg ttaaatcgtg ggtaaagagg tcgctggcaa
     4381 accaggtaaa tttgctcaat gcagtatcga taacgttttt atactgtgcc cgaccggcga
     4441 caatgccgtc atttgaaaca taatccgcgc gggtgatatc agactggcgg cgcgataaac
     4501 cggcggtaac gccaaagtta tcagcgagtt cctgattaaa cgacaaggta taaaagttct
     4561 ttttaaaatc tggagagtaa taagtacttc ctgaagaacc ttgattaaat gcgctcttgt
     4621 tattctcatc gatatgcgat gttaaccagt ccgaacgcgt agtgcgataa cccagtttca
     4681 cgttgctatc atcagcgttg aagcgtttga tctttgcatc aattaccccg ccattgaagc
     4741 gaccgaattc aaccggcaca aaactgtcat aaagcgtcac attgtccagt aagctgacat
     4801 caagataata cccctgtgac atcccgctaa tattggttgc actactggca tcggactcat
     4861 tcgctgggtt caggttatta gtggcactaa taccatcaat caaataggca ttctggtagg
     4921 gcgacgcacc gtgaatagag attttctccg ggcgaatatc tccctggttc aacgaggtac
     4981 tttgcgttga atccatgcgt accgcagggt tggttctcag cagatcgctg atattgccat
     5041 tcccggtcgg taaacgctcg atgctttcac tggtgtaatg cgtgttgccg tttacgggta
     5101 cagggacagg agcatagacc gtcatctctt caaaaaaatg catgtacatg tttttatcat
     5161 cgacggcctg cgccacatca gcgccacata aaatgacgcc aggaataaga actcgcttca
     5221 tttatttata tctccgtaca gcagggcttt tattgttttt ataaaaccgc gctaatgtca
     5281 caaatatcat cggccaggtt ccagacaccg ggttgatgtg taaccataat gacgccgctt
     5341 gtgggtagtt tttcacgcac taaacgcagt aagcggatag cctcttgttc ctcaagatga
     5401 gaggtagttt cgtcaagaaa tatccatttc ggacgtcgta aaattaatcg tgccagggcg
     5461 atacgttgtt tttcgccgct ggaaagaata tctccccagc gatcgtggtc atgaatacgc
     5521 gcagccaatt tcccaagacc aacctgatgc agtacttcgc tcaacgattt atcgtctacg
     5581 ggcaggggaa gtgctttaca aataatctct ttcagtaagc cggttttgat taacggtgtt
     5641 tgtgacacat accaactgtc agcaggagaa gaaatatcac ctttaaacca cggccagcag
     5701 tgggataatg ttttaagcag tgtggttttt cccgcaccag agtaaccttt cagtaatagc
     5761 catttgcctg gcggaacatg aaagttcagg ttctctaata tgatcttatt atcaggggta
     5821 cgaatactcg cgtcagccac ttgtaccgca tgttggcaat ttttaggctt attcgtaggg
     5881 cgctgttcgg tgagttgatg gaactcatac aagcgatcga taaccgcagc cagttcagca
     5941 agttcgtcat atttataaat aaaccagctt aaattgttcg ataccagcat aaatgcctgg
     6001 cgcgatttca tcagtccgcc cagattgatc tgtccgctaa taaactgcgg caataacaga
     6061 aagtacggaa gaacgctaag cgaacgcgaa taaatattct gccagtaatc aagccaccgc
     6121 tgacgattca ttaaacgatg ccagttctct ttaatggtat gaaaattgtc gctcaactcc
     6181 tggcgttgta gagactcagc attgcttagc gcaatcagtt ctgcctgttt attatgctgc
     6241 acaagattag ttcgaaatgt cgcttcgcta cgttgttttt ccacattaag tggacgaata
     6301 cgtttaccca ccttatgggt aaataaagtt ccaccgatca caatgagcac gacggtatag
     6361 accatatacc cctggatatt ccattctgtt ccaccaacag tgaatgagag cgtacccgcg
     6421 ctttgccaaa gaataacggt aaaggtgatc agcatgctaa gtgactggat gaagccaaat
     6481 gaaagactga gcgttttgct gatcagtaag agaatgtctt cagcgatacg ttggtcaggg
     6541 ttatctgtat ttttatgctc gccgtagatc tgcgtgaagt agtagttttt atctgcaaac
     6601 caccgattaa ggtagtaatc tgttaaccat tcgcgccagc gaatagtcag cagtttaatt
     6661 aaccatgttt tgtttacgga tattaataca aagatcccca gcagggcagg gaaccaaagg
     6721 acaagctgcc aaagcttatc ggtttctttc tggcttagcg cattgaaaaa atcattattc
     6781 cagtcgttta accagacctg aattttaacc acgccaagga tcatggcgag gataataatg
     6841 attaacaaca ctgaagtttt attattctta cgcagccaaa agggcttcag cagacagaga
     6901 tactttgcta tcaacataca aaacgtaatg ggaatggtta tcattagcga aaattgattg
     6961 tgccatccaa tgattatcta tggcaatgat tt
//
LOCUS       DAZFKF010000183         6908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFKF010000183 DAZFKF010000000
VERSION     DAZFKF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.185"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(327..893)
                     /locus_tag="ACHKA1_005000"
                     /pseudo
     CDS             complement(327..893)
                     /locus_tag="ACHKA1_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
     gene            958..1626
                     /locus_tag="ACHKA1_005001"
                     /pseudo
     CDS             958..1626
                     /locus_tag="ACHKA1_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224853.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
     gene            2041..2922
                     /locus_tag="ACHKA1_005002"
     CDS             2041..2922
                     /locus_tag="ACHKA1_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003826116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbaSI family restriction endonuclease"
                     /protein_id="HFO5682535.1"
                     /translation="MDKTDYILRSLTKTSKKRWEHYAVTRIYHLLNDPDIEFVCQQCV
                     RKENGKIYLADLFFPQLELYLEIDEGHHNSEQSQIDDAKRRMDITEVTGFLEKRIPAS
                     EISLEIFDRYINEFIELIRNRKNRMIKDKKFKEWDYDNKYTARPHLKAGRISVGPDAV
                     FRYHRDALNCFGYNKGHHQSGGWSIPSHISEAMGLSGKCMVWFPKLYGNSDWGNELTD
                     DGNKIIERNIKDDEGYTERWDKRIVMAHSRDELNRTLYRFLGVFEAIPGHHTGNILEF
                     HRVSTSVNVYPPNDAAP"
     gene            complement(3233..6571)
                     /gene="ypjA"
                     /locus_tag="ACHKA1_005003"
     CDS             complement(3233..6571)
                     /gene="ypjA"
                     /locus_tag="ACHKA1_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFO5682536.1"
                     /translation="MNRISRYYFHRSVLSLLIAAMIYAPPGMTAFTSNVIGVVNDETV
                     DGSQRVDERGTTNNAHIINHGNQEVYGGISNGSVIDTGGHQEVSGHGSYQGQANNTVI
                     NGGSQTISEGGISTGTIINDKGTMSVLTNAKADATRIDNGGAMDVAGNATNTIINGGT
                     QNIYNHGIATGTNINSGTQNIKSGGKADTTNISSGSKQVVEKGGTATGSNIRAGGTLI
                     VDTGGIAHGVYLDTGSALVANTGAGTDIDGYQRSSHFTITGGRAEHVVLENTGELTVV
                     AQTSAVDTIVDAGGKLIVHEEAVAYTTRLNNGGTLDVREKGSATGILQSTQGALVATT
                     RATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTTSAKTQVNTGGREIVKTKA
                     TATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTLTVNDNGKATD
                     IVQNSGAALQTSTANGIEISGTHQYGTFSIAGNLATNMLLENGGNLLVLAGTEAHDST
                     VGKGGAMQNLGQDSATKVNSGGQYTLGRSKDEFQPLARAEDLQVAGGTAIVYAGTLAD
                     ASVSGATGSLSLITPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLADLTAASRGSVW
                     LNSNNSCAGTSNCEYRVNSLLLNDGNVYLSAQTAAPATTNGIYNTLTTNELSGSGNFY
                     LHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGGDASFTLGNTG
                     GFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPNPNPKPDPKPDPKPDPKPDPTP
                     DPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASPHNNNVWGATY
                     NTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSHIGFDRGGHGS
                     VGSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYHSNGLGGHIET
                     GMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMKSKSVDTRSIYRELGATLSYNMRLG
                     NGMEVEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKASFSSTLSGHL
                     GVGYSNGADVESPWNAVAGVNWSF"
     gene            complement(6636..6755)
                     /locus_tag="ACHKA1_005004"
     CDS             complement(6636..6755)
                     /locus_tag="ACHKA1_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO5682537.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(6716..6901)
                     /gene="ymgJ"
                     /locus_tag="ACHKA1_005005"
     CDS             complement(6716..6901)
                     /gene="ymgJ"
                     /locus_tag="ACHKA1_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO5682538.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
BASE COUNT         1752 a         1767 c         1603 g         1786 t
ORIGIN      
        1 atcacaatcc aaaaattaaa atacattttt acaaacgcac cattcttttc gtcccagtat
       61 tagtgaaact agctacgtac tgcatacctt aagtttatgt gacatacaaa aaataaaaca
      121 cccactccgc gcgcaatgct ctccccgcca cgcctgcccg cttaagaggt cgcttttaat
      181 gcaggtgcat gaaccggctc aggccgcgcc ggtactggcg cggtgaggaa aataaaatac
      241 ggggatttgc atgaaaaacc atgcacctta tggatgcatg gcttttttcg gaaaaaatag
      301 cggaattttg cggatttttt tgcgtgctac cgtgcggcca gttctgcacg tcggcgggtg
      361 taaatcaggt tctgtgccgg ggtgaatttc tggcgattat catcccgcga ggccgcatca
      421 ggcctgaatc cgatggccgt taaaatatcg ctgtcctgcg cggaataatt aattttttca
      481 cctgcagcca gccataccga cagggcttca cgcagataat caaccgagtg ctgcatggcg
      541 ctctgtttta ctgcgggaag ctgttcgtta taccccatca gctcaggtgc cagcgtggcg
      601 gccagctccg cgccgtgcta ctgcataaag tcatggagcc ggttgcggat gctgatgtgc
      661 tgcacttcct catgtgagcg gatataacga ccggcggcct gattaatctc ccattttttc
      721 acgtcgatat tgtcgcgcaa atcctgcatt ctgcgcggga tttgttcgtc accggcaagg
      781 agctgttcac ggtattcccg ttcaaggtct gccagttcgg ctttacgttt cagccaggta
      841 tttttgttcg tctgacaggc ctcaaaggcc tgctgtatgg tcacagtggt cacgagtctc
      901 tctcctgatt aatgccggaa cggcgagctg tagcagccct gtactttacg cggcggcggt
      961 gacggcgcag ggcttaaggc catcaccggc ggcgatgcgg tctcggttga cccgaaatac
     1021 cagaacccgt actcaacaca cattccggcg gtgattctgg cagtgaacaa taaccccatg
     1081 cgcttcaccg accgcagcgg tggtgtgtca cgtcgccggg tgattattca cttcccggag
     1141 caaattgcgc cggaggaacg cgacccgcag ctcagggata aaattgcgcg cgagctggcc
     1201 gtgattgtgc gccagctcat gcagaagttc agcgacccga tggcagcctg cgcactgctc
     1261 cagtcacagc agaattccga cgaggcgctc agcatcaagc gcgatgctga ccctacgttt
     1321 gatttttgcg gctatctgga aatgctcccg cagaccaacg ggatgtttat ggggaatgcc
     1381 agcatcatcc cgcgtaatta tcgtaaatat ctctatcacg cgtatctggc ctatatggag
     1441 gctaacgggt ataggaacgt actcagcctg aaaatgttcg ggctggggct gcccatgatg
     1501 ctgaaagagt acggcctgaa ttatgagaag cggcacacta agcaggggat acaaaccaat
     1561 ctgtcgctga aagaggaaag ctacggcgac tggctaccga aatgcgacga acccgtagca
     1621 acataacctc actcagaccg gcaacagccg gtcttttcct ttctggccat tgccacaagg
     1681 tgaacaatcc actgttcacc cttcaccgta tcttcaccct gtatcaccat gaaattatta
     1741 ataaaaaacc agaggtgaac agtgtgaaca gtaaaaccta aaaaaatttt ttaccccccc
     1801 caccacatcg cctgaccggg cagatccaga acgagcaaaa atcacaaagg ggaagattcg
     1861 acttttcact cctcaccaac tcaccaccgc ttaaccacat gatataaaat agaaaatcat
     1921 cgaagttaac agtataaaca gttagattaa aaaaatacta acgcatctta tggcgatagg
     1981 taacactatc gccataagaa aaagcacatt acatgaagaa caaagaagga aggcatataa
     2041 atggataaaa ctgattacat cctacgttca cttacgaaaa cctcaaaaaa acgatgggaa
     2101 cattatgcag taactcgcat ttatcatctt ttaaatgatc ccgacattga atttgtctgc
     2161 cagcaatgtg tcagaaaaga aaatggaaaa atctatttgg cagatttatt tttcccacaa
     2221 ctggaacttt atcttgaaat tgatgaaggg catcacaaca gtgagcaatc acaaatagat
     2281 gatgcgaaac gtcgtatgga tattactgaa gttacaggat ttttagagaa aagaattccc
     2341 gccagcgaaa taagtcttga aattttcgat cgttatatca atgaatttat tgagttgata
     2401 cgcaatcgta aaaatagaat gattaaagat aaaaaattca aagaatggga ttatgataat
     2461 aaatatacag ctcgtcctca tcttaaggca ggaaggatat ctgtaggtcc agatgccgtc
     2521 ttcagatacc accgggatgc tttgaactgt ttcggttaca acaaaggaca tcatcagtct
     2581 ggcggttgga gcattccttc tcacataagt gaagcaatgg gtctatcagg aaaatgcatg
     2641 gtttggtttc caaaactata tggaaattca gattggggaa atgaattgac tgatgatggt
     2701 aacaaaatca tagagcggaa tataaaggat gatgagggat acacagaacg ctgggataaa
     2761 cgaattgtta tggcacattc tcgtgatgag ttgaacagga cactgtaccg atttcttggg
     2821 gtgtttgaag ctatccctgg tcaccatact ggcaacatac tagagtttca tcgtgtaagt
     2881 acatcggtca atgtataccc tcctaatgat gctgccccat gattaacagc atatttagtt
     2941 ccctgtttat acgagataca acactgctac agtgagaaaa ttggtacacg tttaggtaca
     3001 caactgaaag ttgaatacga aaaaatctct tacttttaat aggttgggaa atttattcag
     3061 actccgccag ccctcatgat tggacggtgt aaggacaaca ccaacaaaaa caggaagtta
     3121 gaagtctcag caggacaccg accagacggt gaggagacat aaaaggatac gcaaaggagc
     3181 cgcggctcct ggtgacatga aagcccacag atgtgggctt tttcgttgat ggtcagaacg
     3241 accagttcac accagccacc gcgttccacg gggattccac atcggcaccg ttgctatacc
     3301 ccaccccgag atgcccgctt aacgtactgc tgaatgaggc tttaatacct gcctggtata
     3361 ttccacgtct gcccgacaaa tcattgacga aattaccgtc attattcacc ttcacccggt
     3421 tatcatcgac aaattctttg cgcacagccg ccttcagcca cggctcaact tccataccgt
     3481 tccccagacg catgttgtaa ctcagcgttg cccccagttc acgatatata ctgcgggtat
     3541 cgactgattt cgatttcatg ccattggata aatgatattc ggggttatca gcggtgaacc
     3601 ccgttaacga tgcatacggc gtcaggttcc agttaccatc ggtaaatcgc atcccggttt
     3661 caatgtgacc gcccagcccg ttgctgtggt aactgccatt ggcggctcca ccgctgctca
     3721 ttttaccggc tacgttactt tcaaaacggt tcagcttcac gacaccgtcc agatagaaac
     3781 cactttcatg ttcccaactg gcatagccgc ccagagaata actgcccaca ctgccatgtc
     3841 ctccgcgatc aaaaccgata tgtgaatggg aataacccat aaaagcgccc agcgtggcaa
     3901 ttccttcagg aatatcatta cggctgtcga tccccactgt cattccggtc agcgtctgct
     3961 caaacccggc acccgcatcg gtggtgacat tattacgggt gttatacgtc gccccccaga
     4021 cattattgtt gtgtggactc gctttcatta tgttcaaccg ctcgcgaatg ctgtttagct
     4081 cagcatcaaa taccaacggt aatgttgcag ccatattgag tacggctgcc gtagaaggcg
     4141 tgatgcgttt ctccggaacg ggtgtcggcg ttggatcggg agtcgggtct ggtttcggat
     4201 ccggttttgg gtctggtttt ggatccggct tcggatttgg gttgggattt gggttgggat
     4261 ccgggttggg tttgacatca ttggtcaggt tccagttgct gttgccatcg cttttcagga
     4321 catactcata ggtcccaaga tcaacgaaac cgccagtatt gcccagcgta aacgaagcat
     4381 cccctccccc tgttttcacc agcgtcatcg catcgtcaga ctgtgggctg acgccggtat
     4441 cctgaacaaa gattttaaaa ttaccagtgg cgttgttgtt gacgaccagt tgatcgcccc
     4501 gggagccagc aacgttggta tgcaggtaga aattaccgct accggaaagt tcattggttg
     4561 tcagcgtatt gtagataccg ttagttgtgg caggcgctgc tgtttgtgct gataaataaa
     4621 cattaccgtc gttaagtagc aaactgttta ctctatactc gcagttgctg gtgcctgcac
     4681 aggaattatt gctgttaagc cagacactgc cccggctggc agccgtcagg tcggcaagcg
     4741 tggtatcgac gccatttccc agagtcaatg tcgcgctatc ggtaatccgg accgcccctt
     4801 caagttttac tggcgtaaca ttatcccgtg gcgtgattaa cgacaggctt cctgtcgcgc
     4861 cactgaccga tgcatccgcc agcgtacctg cgtagacgat tgccgtcccg ccagcgacct
     4921 ggagatcttc tgcccgggcc agaggctgaa actcatcttt tgaccgccca agggtatatt
     4981 gcccgccaga gttaaccttt gtggcggagt cctgacccag gttttgcatt gcaccaccct
     5041 tgccaaccgt tgagtcatgg gcttcggtac ctgctaatac caataaatta ccaccatttt
     5101 ccagcaacat attggtcgct aaatttccgg caatggaaaa ggtgccgtac tggtgagtac
     5161 cgctgatttc aataccgtta gccgtgctcg tctggagagc ggcaccgctg ttctggacga
     5221 tatctgtcgc tttgccatta tcgttgactg tcagcgtacc gccttcattg atcgttgttt
     5281 ttattgcctc accgttagcc gaaactgttt gtattccgcc gtcgttaatc gttgtctcat
     5341 tcgccacacc ctcgacgatt tgttcgccgc cggtgagcgt cgtgcctgtc gcagtggctt
     5401 ttgttttgac gatctcccgt ccgcccgtat tgacctgtgt tttggcggaa gtggtgtctg
     5461 actccacggt taacacgccg ccatttgcca gcaggatatt gttcgccgca ccctgctcga
     5521 tgctgaacgc gacgccatca gcgcgtgtcc ctgtgacacg cgtcgccctg gtggttgcaa
     5581 ccaacgcgcc ctgagtactc tgcagtatcc ccgttgcgct gcctttctcc cgcacatcga
     5641 gtgtgccgcc attgttaagt ctggtggtat atgctacggc ctcctcgtgg acaatcagct
     5701 taccgccagc atcaacgatg gtatctaccg ccgaggtttg agctacgacc gtcagttcgc
     5761 cggtattttc cagcacaaca tgctcagccc tgcctccggt aatagtgaag tgagatgacc
     5821 tctggtatcc gtcaatatca gtccccgcgc ccgtgttggc aactaaagca ctccccgtgt
     5881 ccaggtaaac cccatgtgcg ataccgccgg tatcgacaat cagcgtgcct ccggcgcgaa
     5941 tattgctgcc agttgccgta cctcctttct caacaacctg cttgctcccg gaagagatat
     6001 ttgttgtgtc agctttcccg ccgctcttga tattttgcgt gccactgttg atgttggttc
     6061 ccgtagcaat accatgatta taaatattct gtgtgccgcc attaattatg gtattagtcg
     6121 cgttcccggc aacatccatg gccccgccgt tatctattcg ggtcgcatca gctttagcat
     6181 tcgttaaaac tgacattgtt cctttatcgt taattatcgt ccctgtagaa attcctccct
     6241 cactaattgt ctggctgcca ccgttaatga ctgtattatt agcctgcccc tgataagagc
     6301 catgaccaga tacctcttga tgtccaccag tatcaataac acttccatta gaaatcccgc
     6361 catatacctc ctgattgccg tgattgatga tatgagcatt atttgttgta cctcgttcat
     6421 ctactctttg gctgccgtct acagtctcat catttacaac gccaataaca ttagaagtga
     6481 aggccgtcat cccaggcggg gcatatatca tggcagctat caataaggaa agtactgagc
     6541 ggtgaaaata atagcgactg attctgttca taaatttcat cctctgaaaa atgaatactg
     6601 agtagcgtct aagtaaccta agctcggctg caacatcagc ccgcaggcac cagaccaggg
     6661 gattcatcct gaagagacag cgcaggtgta ttgtgttcgc cgctcaccaa agacatcatg
     6721 atgaaatgat gatatcccgc ataagaaaga ggcatttttt aaacgcagtg cgctgaagtg
     6781 tagttggata aaaaatcact cttttcagat aatactgtcg tacgcttatc tttcgacaat
     6841 gaggccgtcg gcaaaataaa atgactaatg taatcgttgc tgatgaatat cttctgctca
     6901 cataaaaa
//
LOCUS       DAZFKF010000184         6767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFKF010000184 DAZFKF010000000
VERSION     DAZFKF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.186"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(97..369)
                     /gene="rcnR"
                     /locus_tag="ACHKA1_005006"
     CDS             complement(97..369)
                     /gene="rcnR"
                     /locus_tag="ACHKA1_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO5682539.1"
                     /translation="MFHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            490..1314
                     /gene="rcnA"
                     /locus_tag="ACHKA1_005007"
     CDS             490..1314
                     /gene="rcnA"
                     /locus_tag="ACHKA1_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO5682540.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1533..1871
                     /gene="rcnB"
                     /locus_tag="ACHKA1_005008"
     CDS             1533..1871
                     /gene="rcnB"
                     /locus_tag="ACHKA1_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO5682541.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1953..2987)
                     /locus_tag="ACHKA1_005009"
     CDS             complement(1953..2987)
                     /locus_tag="ACHKA1_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO5682542.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYGELSAQHNLVVQGDFALTQNQ
                     TATHTYTFNDTSCVSTNTVAQIDPSDIVIGLYNDTIKLKLHFEWEKKGSITLSNNQTS
                     FTSGYNVTVTSAASDAIVNLSGGGSGSLMINSVATLSSASSSTRLAAFLQFAGCFLGG
                     TSWDICVQNYKNALSKNAGVYSFNLALSYNPITTTCKPDDLLITLESIPILQLPTTGN
                     KATINSKQGDIILRCKNLLGQQNQTSRRMQVYLSSSDLLAGSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKIKSGALE
                     ATALINVKYD"
     gene            complement(3001..5481)
                     /gene="yehB"
                     /locus_tag="ACHKA1_005010"
     CDS             complement(3001..5481)
                     /gene="yehB"
                     /locus_tag="ACHKA1_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO5682543.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREIIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGAWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     STPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAASYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(5497..6171)
                     /gene="yehC"
                     /locus_tag="ACHKA1_005011"
     CDS             complement(5497..6171)
                     /gene="yehC"
                     /locus_tag="ACHKA1_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5682544.1"
                     /translation="MKGLSSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(6251..>6767)
                     /gene="yehD"
                     /locus_tag="ACHKA1_005012"
     CDS             complement(6251..>6767)
                     /gene="yehD"
                     /locus_tag="ACHKA1_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5682545.1"
                     /translation="VFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSVSIN
                     MPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAIA
                     SSNDTVGYYLYLGDNSEVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDSSVNSGDV
                     LSSLVMRVAQD"
BASE COUNT         1855 a         1616 c         1335 g         1961 t
ORIGIN      
        1 tgcatactaa taggtaaaat caatatgctt agatatactt tcaacgaata acactatctg
       61 tataatgctc tgattggtga tagttataac tcctatttat ttgatatatg aatccagcac
      121 cttcagaacg acatccagat cttcttcacg ttttagctca tccccctggt gaacgatgtg
      181 ttccgtcaga tgacctttaa tcacttcccg catcagaccg tttaccgcgc cacggatagc
      241 agcaatctgt tgtaaaactg cagcgcattc gtgcggctcg tcgagcattt tcttgagcgc
      301 cacgacctgg ccctgaatct tactggcacg cgctttcagt ttctgtttat cacggattgt
      361 atgaaacatg gcaacacctg gttaacaaga atatgaaaaa tcatagcact attaatctac
      421 tggggggtag tatcaggtac tgggggggag tagaatcaga ttgccgaatt aatactaaga
      481 attattatca tgaccgaatt tacaactctt cttcagcaag gaaacgcctg gttcttcatc
      541 cccagcgcca tcttacttgg tgcgcttcat ggcctggaac cagggcactc aaaaacgatg
      601 atggcggcgt ttatcatcgc catcaaaggc accattaaac aggcggtaat gctcggactg
      661 gcagcaacca tttcgcatac cgcagtggtc tggttgattg cctttggcgg gatggtgatc
      721 agcaagcgct ttactgctca atcagcagaa ccgtggctcc agctgatttc cgcagtgatc
      781 attattggca ccgcgttctg gatgttctgg cgtacctggc gcggcgaacg caactggctg
      841 gagaatatgc acgagcatga tcatgaacat catcaccacg atcacgaaga tcaccacgac
      901 catggacatc atcaccatca cgaacatggc gagtatcagg atgcccatgc acgagcccat
      961 gccaatgaca ttaaacgacg ctttgatggt agagaggtca ccaactggca aattttgtta
     1021 tttggcttaa ccggtggcct tatcccctgc ccggcagcaa ttaccgtgct gttgatatgc
     1081 attcagttga aagccctgac actgggcgca acactggtcg tcagtttcag cattggcctg
     1141 gcgttaacgc ttgtcaccgt aggcgttggc gcagcaatca gcgttcaaca ggtcgcaaaa
     1201 cgctggagcg gatttaacac tctcgctaaa cgcgccccct atttttccag tctgttgatt
     1261 ggcttagttg gtgtgtatat gggcgtacat ggcttcatgg gcataatgcg ataactcaat
     1321 cctttaggct tgcatgttat cttcagccca ggatgattct taaatcagct atttccgctg
     1381 actctctgct cgataaggat gcttttaggg catcctttca tttacacttt ttacgaaatc
     1441 atgggatcac taacaaaata tcgcttgtca gttatattgt atggcaggaa agatatgcga
     1501 ctgatattac agatccccaa agtggagagt ttatgaccat taaaaataag atgttgctgg
     1561 gtgcgctttt gctggttacc agtgccgcct gggccgcacc agccaccgcg ggttcgacca
     1621 atacctcggg aatttctaag tatgagttaa gcagtttcat tgctgacttt aagcatttca
     1681 aaccagggga caccgtacca gaaatgtacc gtaccgatga gtacaacatt aagcagtggc
     1741 agttgcgtaa cctgcccgca cctgacgccg gaacccactg gacctatatg ggtggcgcgt
     1801 acgtgttgat cagcgacacc gacggtaaaa tcattaaagc ctacgacggt gagatttttt
     1861 atcatcgcta aaaaaagccc cctcatcatg agggggaaat gcagacacct tgttattttt
     1921 tattattagc cacttgctcg tcttgcttgt tattagtcgt atttcacgtt gattaatgcg
     1981 gttgcctcca gtgcgccaga tttaatttta tttgtatcgt agacgtagta actggcagtt
     2041 atcggaattg aaaccgttgt atctgtaagt tttgactttg ccgcaatttc ttttaaattt
     2101 gtgtatcctt tactgctgtt agtgatattt aaaagtgtga ctggcgaacc attactttca
     2161 agaataaatc cgactccatt atcttctgca cctttcagga tcgtgttact gccggctaac
     2221 aaatcagaac ttgataaata cacctgcatt ctccgtgatg tttgattctg ctgacctaat
     2281 aaatttttac agcgcagaat aatatcccct tgtttactat ttattgttgc tttgttacct
     2341 gtggttggta attgtaaaat tggaatactc tctaaagtaa ttaataaatc atcaggtttg
     2401 caggtcgttg ttatcgggtt atatgacaat gccagattaa aggaatagac acctgcattt
     2461 tttgataatg catttttata gttttgcaca cagatatccc atgaggtgcc gcctagaaaa
     2521 caacctgcaa attgtaaaaa agcagccaaa cgagtagatg atgaagcact gcttaatgtc
     2581 gcgacactgt taatcatgag agatccactt cccccaccag acaaattcac tatggcatcg
     2641 ctcgctgccg atgtcacagt gacattatat ccactcgtaa aactagtttg gttatttgaa
     2701 agcgtgatgc tacctttttt ctcccattca aaatgcaact taagtttgat ggtgtcatta
     2761 tagagtccaa tcacaatatc cgaggggtct atctgcgcta ctgtattagt actgacgcaa
     2821 gaagtatcat taaaagtata tgtatgagtt gctgtttgat tttgagttag cgcaaaatcc
     2881 ccctgaacaa ccaaattatg ctgtgctgac aactcgccat aacatgcagc ataactcaca
     2941 ggcataacca tcatcattac tataaataac attatacgaa tattcataac cacctgacta
     3001 ttactggcaa atataattcc tactttcatc aatctcttta ccgaaggtga ttgtgcatga
     3061 aagtccttgt tgcttatcaa ttgctacatt aaccgatggc ggtacttcat tggtgcgaat
     3121 aaataactga cttccctggc cgacaacgcc aatattatga ccatggatat cattgacttc
     3181 ataaccaaac gttaatggtt gcccatccgc tcttaacgct tttataaacc agggcttgcg
     3241 ctgatcggta tcaaaattaa ccagtacaac cgcgccgcga taagaggcgg caattttccg
     3301 gttaccgcgc aattctgctt cgctatcgct ttgcgacaca tccagcatca ggtgattttc
     3361 ccgataaggt gtcattccgt cgtataccac cactccatta cggtttgttg tgcgatattt
     3421 ttgcccattg acataagcat ctttaattcc tggcgcattc atcacagcaa acgtttcgga
     3481 aagacggttc gccagattaa cgccacccga ccaggcgaca atgccccctg aaacactggc
     3541 tccagcctgt cgataagtac tcgactgact ataactgcca ttcactgtcg caaccggcgc
     3601 gttccaggtt aaattcgctc cagctgtcgt ttcatttccc tgatgttgat gactcaggtt
     3661 gacaccataa ttgaactgat cccgactccc tactgttcct gataatcccg tattatttga
     3721 ggcaaacccc tgatcatcaa acgtcgttga gttagacata tatatttgcc gacgaggcgt
     3781 cgaaacgtca tcaccccaat caaagggaat cgatataaaa atattaaaac gtttctcttc
     3841 atgatgattc tcgtcataag cctggcttgc cgcgagggta tagcttatcc gtcgcaggtt
     3901 gttggaataa ctcaactgat aatccttact actgccgcta cgcccccagt aatctcgcca
     3961 taacgtactt aatgacacag aaccccagcc ttctggcaat gactggctca tattggcaga
     4021 aaagctattt ttacggccaa aatcgttctg gtaataatcg gcaatgtcat agacatcgtt
     4081 ttcatcacgg cgataattat ctttattgtt agcccaaacg tgatcattaa atgtccgata
     4141 atcacgcgac gaataacgcc aggccgccag accaaaacgc gtcgacgttt ggctcacaaa
     4201 tttgttgtag gcaatttgat aactttgccc gtcaaacaca tcgccgttgt cttgtttact
     4261 atgcgacttc gtggcatcga cggaaatggc accaatgcgt gtattccagc cagtccccaa
     4321 agtgaacgcg taataattat tcgcgaccat cgagccacca tacagcgtca ataagttatt
     4381 aaaaccatac tgataacccg cctggacaaa atcactttgt ttgctcgccc cttcaatatg
     4441 gctacggccc gccgcaaaat catatttcga cacgccgggt tgcagcatat ttggcacggc
     4501 tgcataaggc accagatagg tggttaccga accgtccgcc tctttcacgc tgacatcaag
     4561 atctgctcca ccaccggcca actgcaaatc tgtaatcgca aacgggccag gaggaacctc
     4621 tttctgataa accacaaaac cattctgttc aatagttacc agcgcgttac tctgagcaat
     4681 cccctgcacc cgtggcgtaa aattttgttt cgagttaggc aacatctgca tatcacgaaa
     4741 caaccgcaca cctctgaagc gaacagaatc aaaaatatcg cttgatgtgt acatatcacc
     4801 cacgcgaagc gtgccgagaa gttgggcaaa tccacgttcc agatacaggg tattgctttt
     4861 ccacgcccct ggattgttat ttgttttact gaaactggca tcagaatgca gttgccaccc
     4921 cagtaaattt aacccgctgt taaaacgtac atatgtactc ttactattac ccgacgcttt
     4981 atagtcactg taatactgac tcacataata agaggtataa aacgcattaa taccccgctc
     5041 ccagttttcc ggtggaacat agccactttc cagttcttcc acccaggcct gcgggacact
     5101 gaaatcgaga cgaaaaaccc cgatatccca ggtatagctc ccaccctgaa caagttgctc
     5161 aaatgttaaa cattgcttac cgctggcgaa gttatcgcta ttaatgccta accgcttgat
     5221 aatttccctt gataaacatg tttcttgcgg gttgtcttta acaatgatct catatttccc
     5281 gcgccattgc ttattgacat aaatatcgat gtcatactgc cccggcaagg gttgattatc
     5341 atcaagacga atatttgcaa cctgctggtc tttcattcca cctatcataa aatgggtatc
     5401 aaaggtttct tcagctgcat aagcttcaat gccgagcaat aacgctacga ttgctgatgc
     5461 aagtggggtc attctcaaca taggcagctc ctgcaattaa attttgtcac taatatagtt
     5521 gccatggtca tcgataatgg tcagatgcca gttatttgca ttattacttt tgacattaac
     5581 actctgactt tctaaggggg caatcataat agtttcataa ttgactttga cattattagc
     5641 tttgacatcc gaaatcgtca cccaattagc tgagtcattt tttatcacca atccattgcc
     5701 actgcgattt accagtaact ttttaaaggt cgctttattt accggagcaa tacccgccgg
     5761 ccggtaaaac aatttgattc tgttttgcat cgcaaacttc agtgcattct taccttcttg
     5821 ttctggacta tttggtggaa tatccagaac attcagataa aaaatgcttt ctttattagt
     5881 gggcagttta ttcggcataa ttttgatttt tacttgctgc ccggaatttg cccctatttt
     5941 tgccactggt ggcgttaaca tgaaaggaac ctgaattttt tctggtggta atgacgtatc
     6001 gccatcatca atccacgcct gcagcagcga agaacggttt ccctggttca tcaactgcac
     6061 catcacttct ttattttctg ccgggtaaat aatgcgcgtc ccgtagataa caattccggc
     6121 atgtgcagga aggaccatag aaaaaatgag taaagatgat aatcctttca ttttaatgcc
     6181 tcttaaatta aaaggccgcc atgggatagc ggccatgttt attacctgga gaaacttaca
     6241 attttattta ttaatcctgc gccacacgca taaccagaga agataacaca tcacctgaat
     6301 tcactgagct atctgtcagt ttcaggtatt ttgctttaaa cggaatagca tattgacctt
     6361 ccgctgtttt ataactctca atattaaaag gtgcagaaac atccaggact tcgctgttat
     6421 cacctaaata gagatagtaa cccacagtat cgttgctact ggctatcgcc ttatcgttac
     6481 cagttatttt atcgttggta atcaacgaga gttttacacc agcaacttct ttcgggcagc
     6541 taatttttaa catgctgctg gttacaccga ctgcatcatc ataggtagaa tatttagctt
     6601 taccttcaaa gacagtggtt ggtacagttg gcatattaat acttactgaa tcaccacccg
     6661 cttcgaattt acacggagat tctgcaatat ttcctttaat ggttaatgtt ccggtatcaa
     6721 cgtctgcagc aaatactcca gcgctcataa aaaaagaaga aaagaca
//
LOCUS       DAZFKF010000185         6727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFKF010000185 DAZFKF010000000
VERSION     DAZFKF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.187"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            191..1375
                     /locus_tag="ACHKA1_005013"
     CDS             191..1375
                     /locus_tag="ACHKA1_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFO5682546.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNVPVVTQPVEILPSIHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            1486..2409
                     /locus_tag="ACHKA1_005014"
     CDS             1486..2409
                     /locus_tag="ACHKA1_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO5682547.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPSALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(2413..3231)
                     /gene="nlpA"
                     /locus_tag="ACHKA1_005015"
     CDS             complement(2413..3231)
                     /gene="nlpA"
                     /locus_tag="ACHKA1_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO5682548.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            3440..3733
                     /gene="yicS"
                     /locus_tag="ACHKA1_005016"
     CDS             3440..3733
                     /gene="yicS"
                     /locus_tag="ACHKA1_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO5682549.1"
                     /translation="MKLTMLLMITVFLTFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDESNKQHIREAIVAIERNNQNNYWEALGKVECPDM"
     gene            4035..4532
                     /locus_tag="ACHKA1_005017"
     CDS             4035..4532
                     /locus_tag="ACHKA1_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone"
                     /protein_id="HFO5682550.1"
                     /translation="MESANLDLEENKEIASKFERALGMGATLAELHGITPDTLEGVYA
                     YAYNFYEKGRLDEAELFFKFLCIYDFQNYNYLKGYAAVCQLKKDYQKAFDMYHICLML
                     SPDNDFSLVYYMGQCQMGLKNIKMATELFNTVVTYSQNEKVKEMATAYLELLTANTEE
                     EVQTE"
     gene            4542..4724
                     /locus_tag="ACHKA1_005018"
     CDS             4542..4724
                     /locus_tag="ACHKA1_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682551.1"
                     /translation="MKINGKNASFTPEELARKYHEEAPVDLKNNHGDKINEKNNNKHI
                     TYFPVDAILDGNKHLP"
     gene            4780..6561
                     /locus_tag="ACHKA1_005019"
     CDS             4780..6561
                     /locus_tag="ACHKA1_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopB/SseC family type III secretion system
                     translocon subunit"
                     /protein_id="HFO5682552.1"
                     /translation="MSAPITGQTITFEQISETLRTQYSDAEKRLQDSSKTQVDPMRLN
                     KNPKSLDNDIRARLENKPMLAPPAIQVSDSDNATTAKTNDARLTMILGNLTGIADQDI
                     ATRLHNNLESTLLRHEMAHNKFRELSDAYSSSLDDAQKADDIMHQANNNYNAVDKKVQ
                     SLEKKVNTLNQELSQLQPGDPQYNKVLTQKNAAEKTLTLSLQKKSLAEKSLNTAIMDA
                     DAAIGQSMEIFDEIQQQEQINNFTTNICLTQENQKNRNATATFILLITSVMEVIGDTN
                     CDSIKNQSEVMKEINHVRENKLNETARKYTTTTKVLKIVNECVTVATIAMSAILIVVG
                     LLAAVPSGGSSIAGALALIGGIAGAVVLGVDITCQIALGTTATGWILGKVVEGLSAAI
                     KTVDPTLLAITALLDVIGVDQDTIELVKSIYASAAASIVMATVMIGAAVICSVAIGAV
                     VSALSKTAAEEVTKEITSTIKSTIESIINSVSKNIIKVLDSVCSMIQTSVVVLKLIAK
                     ISNGLEKIGLLICETATSIMNYCVAENSADMAILQQDMSNLSKTREQMLSVLQRVDKT
                     VEQEVSQMVRVLQHRTEALKFASHSIV"
BASE COUNT         1966 a         1433 c         1429 g         1899 t
ORIGIN      
        1 ttaatatata aattcggtga ttaattctta acatgctttt gctaataatc tcaattgctg
       61 gccctataat attgcgctag cattgttttc tggttgtatc agcgatactc aaaaattctt
      121 aatacaatac tcattcgact ggtacttatt tgtaactcag ttatattttg tcgcccggtg
      181 attcagaaga atgcaaaaaa cggctaccac tccatcaaaa atacttgatc tcactgccgc
      241 ggcattttta cttgtcgcct ttctgacggg tattgcgggc gctcttcaga ctcctaccct
      301 aagtatattc ctcgcagatg aactgaaagc ccgtcctata atggtaggtt ttttcttcac
      361 cggtagcgct attatgggaa ttctggtcag tcaatttctg gcaaggcact ccgataaaca
      421 aggcgaccgt aaattactga tcctgctatg ttgcttattt ggagtgctgg cctgcacgct
      481 ttttgcgtgg aatcgcaact acttcattct gctctcaacg ggcgtacttc tgagtagttt
      541 tgcttccacc gcaaacccgc aaatgttcgc cctcgcccgt gaacacgccg atagaacagg
      601 ccgtgagacg gtcatgttca gtacattttt acgtgctcag atctcgcttg cctgggttat
      661 cgggccaccg ctcgcttatg aactggcaat gggatttagt tttaaagtga tgtatctcac
      721 cgctgccatc gcatttgttg tttgcgggct gatagtctgg ttgtttttgc catcaataca
      781 aagaaatgtt cctgtcgtta cccaacccgt agaaatttta ccctccatcc atagaaagcg
      841 agatacgcgg ctactttttg tggtctgttc aatgatgtgg gcggcgaata atctctacat
      901 gataaatatg ccgctattta ttattgatga actgcatcta accgataaac tggctggaga
      961 aatgattggt atcgctgccg gtctggaaat tccgatgatg ttaatcgcag gctattacat
     1021 gaaacgtatt ggcaagcgac tattaatgct cattgctatc gtgagtggaa tgtgttttta
     1081 cgccagcgta ctcatggcga cgactccggc agttgagctg gaattgcaaa ttctaaatgc
     1141 catcttcctt ggtattctct gtggtatcgg catgctttat tttcaggacc tgatgcctga
     1201 aaaaataggc tctgcgacaa cgttatatgc aaatacttca cgcgtcggct ggattatcgc
     1261 cggctctgtt gacggaatta tggttgaaat ctggagctac catgcgttgt tctggctggc
     1321 gatagggatg ttgggtattg cgatgatttg cttgctgttt attaaagata tttagctcac
     1381 cagtctgggc taagaaaaat cggcgtggcg caaacagagc catcctcgcc atttttgcta
     1441 tcatgcctgc atacataaac gacaaaacag tatgcagagg gaaaaatggg ttccaccaga
     1501 aaggggatgc tgaacgttct gattgccgcc gtgttgtggg gaagttcagg ggtctgtgcg
     1561 caatacatca tggagcaaag ccagatgtca tcgcagtttt tgactatgac gcgtttgata
     1621 ttcgccggtt tgattctgct gacgctgtca tttgttcatg gcgataaaat cttttctatt
     1681 attaacaatc ataaagatgc cattagtctg ctgatttttt ccgtggttgg cgcgctaacc
     1741 gtacagctca cttttttgct aaccatcgaa aaatcgaatg ctgccacggc aacggtgctg
     1801 caattcctct caccgacgat tatcgtcgcc tggttctcac tggtgcgtaa atcccgcccg
     1861 ggcattctgg ttttctgcgc tattttgaca tcgctggtcg gaactttttt attggtgaca
     1921 cacggtaatc cgacgtcatt atcgatctct ccttccgcgt tgttctgggg cattgcctcg
     1981 gcatttgctg ctgcattcta taccacctat ccctcaacgc tgattgcccg ctatggcacg
     2041 ttaccagtcg tcggctggag tatgctgatt ggcggtctga ttctgttgcc tttttatgcc
     2101 agacaaggga caaactttgt cgttaacggc agtttgattc tggcgttttt ttatttggtg
     2161 gtcattggta catctctgac atttagcctg tacctgaaag gagcacaatt aattggcggt
     2221 ccaaaagcca gcattttgag ctgtgcagaa ccattaagta gcgcgctact ctctttgctg
     2281 ttgctgggga tcacgttcac attaccggac tggctgggaa cgctgctgat tctgtcatcg
     2341 gtaattttga tttcaatgga ttcccgtcgc cgcgccagaa aaataaatcg tccggcgcgg
     2401 gatgagtgag aattaccagc caggcaccgc gccaccgtta aagatggttt ctgccgcttt
     2461 agcgacttcg gatgattgat aagattgcag aaattccttc acgttttctg cattcttatt
     2521 atcttcccgt gccaccaaaa tattcacata cggcgaattt ttatcttcaa taaatacgct
     2581 gtcgtgcacc ggagaaagcc cggtctgctg aatgtaagtg gtgctgataa tcgctacatc
     2641 aactttggga tcatccagta cgcgcgggag ttgcgccccc tcgagttcca taatctgcaa
     2701 atgacgagga ttatcggtta tatccagcgc ggtaggtaat aacccttttc cctctttcag
     2761 agtaattagc ttctcttttt gcaacagtaa aagtgcgcga ccaaggttgg tcgggtcgtt
     2821 ggggatcgcc actgtcgcgc cttcttttat ttgcgccacg gttttgattt ttttggaata
     2881 acccgccata gggaagacaa acgtatttcc caccgctacc agtttataac catgcgcctg
     2941 attatcttgt tcaagaaagg gtcgatgctg gaagacgttc gcatcaagtt cgccatgatt
     3001 tgtcgcatcg ttcggtagca acgaaccgct aaaacctacc agttcaacat cgaggccata
     3061 tttctctttt gccacctttt tagcgacttc cgcgacatct tgttctgcgc catttataac
     3121 gccaacttta atgtgttttg catcgctgct gctctggtcg caacctgcca gcagaattcc
     3181 tgccagcaat aatgcggccc ccgcccgtag atgatgtgtt gtcagtttca ttttttatcc
     3241 tttttttaat cattaactga ctataatggc aagatcacta cgatttaaaa aacgaaaagc
     3301 tatcgaatag aagaaaaagg tataaaaaac tggcacttct tactgaccac gcaaacacct
     3361 ttcgtacagc atgatgagag cggcaaaagc aaactacact tatgcttcat tcgatgcaca
     3421 tttgcagaag gttgccgcta tgaagctaac gatgctactc atgattaccg tattcctgac
     3481 ttttccagcc atttctcagg ccgagtcgcc attcagttcg ctacagtcgg caaaagagaa
     3541 aaccacggta ttgcaagatt tacgtaaaat ttgcacgcca caggcgtcat tatcagacga
     3601 agcgtgggaa aaattaatgt tgtctgatga gagtaacaaa caacatatcc gcgaagctat
     3661 tgtggcaata gagcgtaata atcagaataa ctactgggaa gcgctgggta aggtagaatg
     3721 cccggatatg tgaatttcgc acacctccgc gctcaggcca gcagccgctg acttaagctc
     3781 gcacatcagt aaatcacctg ctttttcaaa ttccaatcaa aattgaacca gttcatcagg
     3841 attgtggata aatttccctc tacattattt tcatttcatc acatagaaaa tcttatctca
     3901 ttgaaacaat acttgttttt aatgcagaaa acgaagccca acggatgggc ataaaaatga
     3961 gcgaattttg atatcaccac cccctaaaaa atggtgaagt ctgagcaaag aatgttcgaa
     4021 aggagagagt agagatggaa agcgcaaatc tggatttaga agaaaataaa gaaattgcca
     4081 gtaagttcga acgtgcacta ggtatgggag caacacttgc agagctgcat ggcattactc
     4141 cggataccct tgaaggcgtt tatgcttacg cttataactt ttatgaaaaa ggaagacttg
     4201 acgaagctga actctttttt aaatttctct gtatttatga tttccaaaat tataactact
     4261 taaaaggata tgccgccgtt tgtcagttga agaaagatta tcaaaaggca tttgacatgt
     4321 atcatatctg tttaatgcta tctcccgata acgatttctc tttggtttat tatatgggtc
     4381 aatgccagat gggcttaaaa aacattaaaa tggccacgga gttatttaat acagtcgtaa
     4441 cctacagtca aaatgaaaaa gttaaagaga tggctactgc ttatcttgaa cttttgacag
     4501 ccaacaccga agaggaagtt caaacagagt gagtcagcaa aatgaagata aacggaaaaa
     4561 acgcttcttt tacgcctgaa gagctagcca ggaaatatca tgaggaagcc ccggttgatt
     4621 taaaaaataa ccatggcgat aaaataaatg agaagaataa caataaacac attacttact
     4681 ttcccgttga tgccatttta gatgggaata aacatttgcc atgagttaaa taaaaaagaa
     4741 ttaattacac ccggtgatag cttaaaaagg aggtgaatta tgtcggcacc gattacggga
     4801 caaacgatca cgtttgaaca aatcagcgaa acattgcgta cacagtatag cgatgcggaa
     4861 aaaagactgc aggacagcag caaaacgcaa gttgatccca tgcggcttaa taaaaacccg
     4921 aaatcccttg ataacgatat tcgcgctcgt cttgaaaaca aaccgatgct tgctccgcct
     4981 gcaatacagg tttcagattc agacaacgcc actacagcaa agacgaatga tgcccgcctg
     5041 acaatgattt tgggtaattt aacaggcatt gccgatcagg atattgcaac acgattgcat
     5101 aataaccttg aaagcaccct gcttcgacac gaaatggcac ataataagtt tcgtgaatta
     5161 agtgacgctt actcctcatc attagatgat gcgcaaaaag ccgacgatat catgcatcag
     5221 gccaataata attacaatgc ggtcgataaa aaggtgcaat cgctggagaa aaaagtcaac
     5281 acattgaatc aagagttgtc acaactgcaa cctggcgatc cgcaatataa taaagtactg
     5341 acgcaaaaaa atgcggctga aaaaacactc acgctgtcat tacaaaaaaa atcgttagct
     5401 gagaaatcgt taaatacagc cattatggat gctgatgctg cgatcggcca aagtatggaa
     5461 atttttgatg aaattcaaca gcaagaacag attaataact tcaccaccaa tatttgcctg
     5521 acccaagaaa accagaaaaa tagaaacgcg acagccacat ttatcctttt gattacctca
     5581 gtaatggaag ttattggtga taccaattgc gactctatta aaaatcaatc tgaggtaatg
     5641 aaagagatta accatgtcag ggaaaataaa ctcaatgaaa cagcgcgtaa atatactacc
     5701 acgactaaag ttttaaaaat cgttaatgag tgtgtaactg tagctaccat tgccatgagt
     5761 gcaatcttaa ttgttgttgg tcttcttgct gcagttccta gtggtggttc aagtattgcg
     5821 ggtgcactgg cgcttattgg tggaatagcg ggcgcagtgg tattaggtgt tgatatcact
     5881 tgtcaaatcg ctctaggcac cacggctacc ggctggattt tagggaaagt tgtagaaggt
     5941 ctttcggctg ctattaaaac agttgatccc actctgctcg caatcaccgc gcttctggac
     6001 gttattggtg tcgatcaaga tacgattgaa ctggttaaaa gtatttacgc aagtgcagcc
     6061 gcttctattg tcatggcgac agtaatgatt ggtgcagctg taatatgttc tgtagcaata
     6121 ggggccgttg tttctgcatt atcaaaaaca gccgcagaag aagtcaccaa agaaataaca
     6181 agcactataa aatcaaccat tgaatctatt attaattccg tttcaaaaaa tattataaag
     6241 gtattagaca gcgtatgcag tatgatacaa acatcagtcg tagttttgaa gttgattgcc
     6301 aagataagca atggcctgga gaaaataggc ttacttatct gtgaaacagc aacatcgata
     6361 atgaattact gtgttgctga aaactctgcc gacatggcaa ttttacaaca ggacatgagt
     6421 aatctatcaa aaacgcgtga acaaatgctt tcagtattgc aaagggtgga taaaaccgtc
     6481 gaacaagagg taagccaaat ggtaagagta ttacaacacc gaactgaagc cttaaaattt
     6541 gcttcccatt ctatcgtata agagggttct aacatggcca cctatgaaat taaaaatgcg
     6601 accagcacac ttaatattaa caatctcgat aaaacaatct ttgatagaga gcatagcaaa
     6661 gaaaacaaca taccaaatga taataaaatt gtgcaatact ataaccttaa agatattaag
     6721 catgaca
//
LOCUS       DAZFKF010000186         6631 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFKF010000186 DAZFKF010000000
VERSION     DAZFKF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.188"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..380)
                     /locus_tag="ACHKA1_005020"
     CDS             complement(<1..380)
                     /locus_tag="ACHKA1_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFO5682553.1"
                     /translation="MIVRYGGGNDGIVDYLINGRKAERQYTRDELDHRVVLDGDLQTT
                     DKIIDSIENKSQERYLHITLSFHESHVSNEVLMAIVDDYKKLLMNAYHPDEYSFYAEA
                     HLPKIRHIQDNSTGELVERKPHIH"
     gene            complement(377..718)
                     /gene="mobC"
                     /locus_tag="ACHKA1_005021"
     CDS             complement(377..718)
                     /gene="mobC"
                     /locus_tag="ACHKA1_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFO5682554.1"
                     /translation="MKKKTNKNVHVTFRLTEEEYAPFDRAIKELNISKSEFFRLLTIG
                     KINTYASDKRNIPEYKRCLSQLSWAGNNINQIAHRLNSDHLKGIISESLYKKVLNGLI
                     GIRDRLQEIAK"
     gene            1048..1527
                     /locus_tag="ACHKA1_005022"
     CDS             1048..1527
                     /locus_tag="ACHKA1_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682555.1"
                     /translation="MNNGENKLLGSLLAQKVKRSKTGRIRERFAEIEEAQQQGIRNID
                     IVNALNDEGFDLTLKTFENILHRIRKERAEKKDVSHLLSNKEKTYQKAITIEDKNRKT
                     KQDNDILNAYLPVCFNNAKIAQQAIDNNVSIETIKSWNCANFVQVSNTLGNYIRNKR"
     gene            1533..2234
                     /gene="stbB"
                     /locus_tag="ACHKA1_005023"
     CDS             1533..2234
                     /gene="stbB"
                     /locus_tag="ACHKA1_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StbB family protein"
                     /protein_id="HFO5682556.1"
                     /translation="MYLKLAIVNNSGNVGKSTICQTLLKPRLEGSEIIKVETLNTDGT
                     NDEKLSAKEFDEIIKRIDDSECTIIDVGSSNIEQFLVQMNEYQGSHDLIDYFIVPVVR
                     EAKQQIDSIQTITTLMAMGVEQDRFKVIFNLAEKDTPINKQYSVFLADDVCKEIVGEN
                     PVVVYHNNIFNILNKSGLQYDDVYNDNRDFRSLIRSASSKEERQDLSNLRAVKMLMNG
                     FNSDLDVAFKNLNLV"
     gene            2281..2880
                     /locus_tag="ACHKA1_005024"
     CDS             2281..2880
                     /locus_tag="ACHKA1_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682557.1"
                     /translation="MSRSISEEFKDQKINDLLEDYIALEKQIEKTANLIINAIDETPA
                     RLDAVLSDKVNKIIQSSIDINKDINIAKSDIRQISDECQSEITKQAINTFSGIKQNFD
                     QIASDHKKQLEILTKSAKPFSIKTAIAICALCTLCLSAAFSGATWYVAQHEKEASLRF
                     YARAFLDMKKITEESMRKLPKSDQQNIKLKLDEIDSRKP"
     gene            2900..3226
                     /locus_tag="ACHKA1_005025"
     CDS             2900..3226
                     /locus_tag="ACHKA1_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682558.1"
                     /translation="MTTFTDDDKKLIKEIRERIGSLDVRDNIERRAYEIALASLEAEA
                     PNLPGGFTIEEAKELHENLVKSHVSKALSGEKMKKENRDADLRWIHGVIVQAAWFVKA
                     SLGQNA"
     gene            complement(3357..3794)
                     /locus_tag="ACHKA1_005026"
     CDS             complement(3357..3794)
                     /locus_tag="ACHKA1_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO5682559.1"
                     /translation="MFFSVGVETPKDDHTAYGITVPVFDCFDFGCVSAADSQAEIPAM
                     AREAILAIVEEMVISGAHSVDDIHDEGCLTYSANPNYNHCDSWFVIDVDLSEIEGKQQ
                     RINISLPDVLIRRIDGYVRESGGVYKDRSHFLAQAARHELAYK"
     gene            complement(3819..4001)
                     /locus_tag="ACHKA1_005027"
     CDS             complement(3819..4001)
                     /locus_tag="ACHKA1_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO5682560.1"
                     /translation="MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKK
                     DLPLGTVRSIKKMAGI"
     gene            4298..4717
                     /locus_tag="ACHKA1_005028"
     CDS             4298..4717
                     /locus_tag="ACHKA1_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682561.1"
                     /translation="MAQPTPEKILAIIDDHDRAQKKQRNKNVFIYLCLCLAIIATFRL
                     DGVAVLTILLGVSAIAFFLHYVESGKGQILSSDRILSILAQTKDYPTIRRKVVNRLLS
                     GSKLTGKDEEYIFSELKKAEQSNEREKRLQAIKEYTS"
     gene            complement(4824..5090)
                     /locus_tag="ACHKA1_005029"
     CDS             complement(4824..5090)
                     /locus_tag="ACHKA1_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO5682562.1"
                     /translation="MKHLKYKGYLGTVEPDFENNILHGKLAFIRDLVTFEAETLADLE
                     REFKTSVDLYLQSCVEDGKEPDAPFKGVFNVRLDPELGTGKRSH"
     gene            complement(5510..5788)
                     /locus_tag="ACHKA1_005030"
     CDS             complement(5510..5788)
                     /locus_tag="ACHKA1_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFO5682563.1"
                     /translation="MHFTTFLKKHFDIEKVVGTSDSGNDTESIYVYEKGNDCEPLFIL
                     HESWLNAEIKKCGVWTIGDIYSTLEHGKEYSEQELIKMIKEGKVISKY"
     gene            complement(6338..6589)
                     /locus_tag="ACHKA1_005031"
     CDS             complement(6338..6589)
                     /locus_tag="ACHKA1_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5682564.1"
                     /translation="MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGT
                     ASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDSGSN"
BASE COUNT         2186 a         1383 c         1217 g         1845 t
ORIGIN      
        1 atatgaatat gtggttttct ttcaacaagt tctccggtgc tattatcctg gatatgacga
       61 attttaggca aatgagcttc cgcatagaaa gagtattcat caggatgata agcattcatc
      121 aataactttt tataatcatc aacaatagcc attaacactt cattcgacac atgactttca
      181 tgaaaagaca gagtaatgtg caaataacgt tcctgacttt tgttttcaat agaatcaata
      241 atcttatctg tagtctgtaa atcaccatca agaacgacac gatgatcaag ttcatcacgc
      301 gtgtactggc gttctgcttt acgaccattt atcagataat caacgatacc atcattacca
      361 ccaccgtatc tgacaatcat ttagctatct cctgaaggcg atcacgaata ccgattagtc
      421 catttaaaac ctttttataa agcgattctg atataatacc ttttaaatga tctgaattta
      481 atcggtgcgc tatttgattt atgttgtttc ctgcccaact caactgagaa agacaacgtt
      541 tgtattctgg gatgttacgt ttatcagatg catatgtgtt tattttacca atagtaagca
      601 gcctgaaaaa ttctgactta ctaatattaa gctcttttat agccctatcg aacggagcat
      661 attcttcttc ggtaagtctg aatgttacat gaacgttttt gttggttttt ttcttcatat
      721 gtcctcaagg ttcgtagaac cgcataagaa cggctgcgtc gttcggggtt tcaaaggggc
      781 atcccccttt ggcacacgat tgtaacatga ccggaacggt cttgtgtaca atcggtatcg
      841 tgcctgtgtt tgagaaaatt atttgattcc atagcattca cacatcaaat gcacaccata
      901 aacatagcta atacatagca aaaacacacc ataaaaacca atgacatagc atatacatag
      961 caaacacata gcatatacat agcaaaaatt gcttgacgtt catgatagaa ttgctcatca
     1021 tgatttcact gtcctggaga ctgacaaatg aacaatggag aaaataaact acttggttcg
     1081 ctcttagctc agaaggtcaa gcgaagtaaa actggaagaa taagagagcg ttttgctgaa
     1141 atagaagaag cgcagcaaca aggcataaga aacattgata ttgtcaatgc tcttaatgat
     1201 gaaggttttg atctaacgtt aaagacgttt gaaaacatat tgcatcgcat acgaaaagaa
     1261 agggccgaga aaaaagatgt ttctcatctt ctttcaaata aagagaaaac gtatcaaaaa
     1321 gcaataacca ttgaggacaa aaatagaaaa acgaagcaag ataatgatat acttaatgca
     1381 tatttgccag tttgcttcaa caatgcaaag atagctcaac aagctataga taacaatgta
     1441 tctatagaaa caataaaatc ttggaattgt gctaactttg tacaagttag caatactctt
     1501 ggaaattata tacgaaataa aaggtaagaa aaatgtactt aaaactagca attgtaaata
     1561 atagcggaaa cgtagggaaa tcgaccattt gtcaaacttt attgaaacct cgtctggaag
     1621 ggtcagaaat tataaaagta gaaacattaa acactgatgg tacaaatgac gagaaattat
     1681 cagcaaagga gtttgacgaa attatcaaac gtattgatga tagtgaatgc accatcattg
     1741 acgtaggctc atcaaatatt gagcaattcc ttgtacagat gaatgaatat cagggaagtc
     1801 atgaccttat tgattatttc atcgttcctg ttgtgcgaga agcaaaacaa caaatagact
     1861 caattcaaac cattacaaca ctaatggcta tgggtgttga acaggatcgt tttaaagtaa
     1921 ttttcaatct tgctgaaaag gacaccccta ttaacaaaca atattcagtt ttcttagctg
     1981 acgacgtatg taaagaaatt gtaggtgaaa atcctgttgt tgtttaccat aacaacattt
     2041 tcaatatttt aaataaaagc ggcctacagt atgatgatgt ctataatgac aatcgtgatt
     2101 ttcgttcatt aattcgttca gcgtcttcaa aggaagagcg gcaagatttg tcaaatttaa
     2161 gagcagtaaa aatgttgatg aatggattca acagcgatct tgatgttgcc tttaaaaacc
     2221 tcaatctcgt ctgatacaat atggccggga tgtacccggt cataaaattg gagaaaaaca
     2281 atgtctcgta gtatctctga agagtttaaa gatcaaaaaa taaacgatct tcttgaagat
     2341 tatattgctt tagaaaaaca gatcgaaaaa acagcaaact taattatcaa cgctatagac
     2401 gagacacctg ctcgtctgga tgccgttctg tcagacaaag taaataaaat aatacaaagc
     2461 agcattgata ttaataaaga tattaatatt gcaaaaagcg atataagaca aatatctgat
     2521 gagtgtcaga gcgagataac caaacaagca ataaatactt tttctggaat caaacaaaat
     2581 tttgaccaaa ttgcttccga ccataaaaaa caactagaga tactaactaa atcagcaaaa
     2641 ccattcagta tcaaaacagc cattgcaatt tgtgccctgt gcacattatg cctgtctgcg
     2701 gcattttctg gcgcaacctg gtatgttgca caacatgaaa aagaggcaag tctgcgcttc
     2761 tatgcaagag catttcttga tatgaaaaaa ataactgaag agagcatgag aaaattacca
     2821 aaatcagacc agcaaaatat aaaactcaaa ctagacgaaa ttgattcaag aaaaccatag
     2881 atatgaaaag gagtcactaa tgacaacttt taccgacgac gacaagaaac taattaaaga
     2941 aatcagggag cgaatcggca gcctggacgt gcgagacaat attgagcgcc gggcttatga
     3001 aattgctctg gcatcgctgg aagcagaagc gccgaatttg cctggtggtt tcacaattga
     3061 agaagcaaaa gaattacacg aaaatctggt aaaaagtcac gtaagcaagg ctttaagtgg
     3121 cgaaaaaatg aaaaaagaaa atcgtgatgc cgatttgcgc tggattcatg gcgtaatagt
     3181 tcaggccgcg tggtttgtga aagcatcact agggcagaat gcatagtgcg tacctgcttt
     3241 tctcctggca acggtggatc ttccgcttcc tcgtcactcg ctcgctatgc tcggtcgctg
     3301 gactgcggcg agcggttagc tatcaaaaaa aatccccgca caacgcgggg atagttttat
     3361 ttatatgcaa gttcatgacg tgccgcctga gccagaaaat ggctacgatc tttgtatacg
     3421 ccgccacttt ctctcacata tccatcaata cgacgaatca atacatcagg caatgaaata
     3481 ttaatacgtt gttgtttgcc ctcaatttct gacaaatcaa catcaattac aaaccagcta
     3541 tcacagtggt tataatttgg gttggcggaa taagtcaaac acccttcatc atgaatatca
     3601 tcaacagaat gagcaccgct tattaccatc tcttccacga ttgctaatat cgcttcacgc
     3661 gccattgcgg gtatttctgc ctgagaatca gcagcagaaa cacatccaaa atcaaagcaa
     3721 tcaaaaaccg gaacggtgat accgtatgct gtatgatcat ctttcggtgt ttcgacacca
     3781 acagaaaaga acatggctac ctccaaagtc ggcggggatt aaatccccgc catttttttg
     3841 atcgatctga cagtacctag cggcaagtcc tttttaggat gaggaacagg aaaagttttt
     3901 cctgttattg gcgaccacca tatctggtga ctgcttgcct tatgccgttt caactcgcaa
     3961 ccagcagcta tcagttcttt caacaggtca gcagatttca tatgtcctcc ctaacctgaa
     4021 aacaattata cacacgcata cacacaaagg caaattattt cattcgtatg acttatgaaa
     4081 cacactgtaa agacagttta atctttacag tgtaaatcat ttatccagtt ttatcgctat
     4141 gcatgtgata catagaaaat catatagtgt tttactatat gatacctaat tgacactttt
     4201 aactgcacat ctcatcattt acatcgtaaa gacaaacata gctttacaat gtaaagctat
     4261 ataattaaac actccgtgtc cctgataagg aaaaaaaatg gctcaaccaa ccccagaaaa
     4321 gatactggca atcatagatg atcatgatcg cgcacaaaaa aaacaacgca acaaaaacgt
     4381 cttcatttat ttatgtctct gcttggcaat tattgcaacg tttcgtcttg atggtgtagc
     4441 cgtccttaca attcttttgg gcgtgtcagc aatcgcattt tttctacatt atgtagaatc
     4501 agggaaaggc cagattctta gcagtgaccg catattgtcc atactcgctc aaaccaaaga
     4561 ttaccccaca attcgacgta aagttgttaa tcgcttgctc tccggttcaa aactcacggg
     4621 taaagatgaa gagtacatat tcagtgaact gaaaaaggct gaacaaagca acgaacgtga
     4681 aaagagattg caagcaatta aagagtatac atcgtaatac aacgtaaagc atcacttgta
     4741 aatacaacac actacatcgt aataccagaa aacaccaacg ccagcgagca acaaaaaggg
     4801 gcgattaagc ccctgcgcga tgattaatga cttctttttc cagtgcctag ctctggatca
     4861 agcctgacgt taaacacacc tttaaagggc gcgtcaggct cctttccgtc ctccacacag
     4921 gactgtaaat acaaatcaac cgatgtttta aattcccgtt ccaggtcagc taatgtttct
     4981 gcctcaaaag tcaccagatc tcgaataaat gccagctttc catgcaggat attattttca
     5041 aaatccggct caactgtacc taaatatcct ttatatttta aatgtttcat aaaaccccag
     5101 cctctttcaa gaaaagttat cagatagaag ggtgaaagaa aataccaggg agcagcgagg
     5161 ccacataccc ctaagcgcag cgcaaccgcc ggaaaacgca ccacactgta aagacaataa
     5221 aatctttaca acgtaaagct aactatgtaa tacaacgcgc tacacaataa gaaagggaga
     5281 gcatgacact ctccccatat atgactcaaa ctaaagcatt atcccggtta tgcgcccacc
     5341 aaatgatttc gctaataatc catgcttcta tcgcgggtgt ttttacaggt tccccattta
     5401 caatgcggtt tgcaagctct atcatttcac atactgacat ttgagaatct ccaaagatgc
     5461 atctgtcatt tggtaacagg ctgccccaca gcctgttacc aaccccaaat tagtatttgc
     5521 tgattacttt accctcctta atcattttta ttagttcctg ttcagaatat tcttttccgt
     5581 gttccaatgt ggaatatata tcgccaatag tccatacccc acacttttta atttctgcat
     5641 ttagccagga ttcatgcaga ataaacaacg gttcgcaatc atttcctttt tcataaacat
     5701 aaatagattc ggtatcattt ccagaatcag aagtaccaac gactttttct atatcgaaat
     5761 gtttttttag aaaagttgtg aaatgcattt tcatgctcct gccgctgagc ggctttggtg
     5821 gttggggacc atcccctccc gatgtgttta taataggtca gaactgacct attatcaagt
     5881 attttcccct ccattcttca aaaaaaacac catcaaatac agacagttac attcatgtaa
     5941 tgcccgacag ggcatggaag gggcgaaaga aaataccagg gagcagcggg gccacatcgc
     6001 cccgaagcgc ggcgcagccg ctgacgaaca taccaggatg cacagggcca tcccttcgcc
     6061 ctgcaaagcc agaacagacc aacaggccat gatcatgctg atttgcgccg cagaatgccg
     6121 cagttagcgc gagcgacagc gagtttacgc cgtcctggtg cattgcagca gggcggggcg
     6181 ctgtactgcg cgtgtgagcg tcagcgagct acgggcgcaa ttgctcattg ttcaatgcaa
     6241 ctggggctat taagtccagc cataaattca gttatggttt ttgtagctta ttccgtgatt
     6301 tcgtcatacg ttaattctat tttcatctta atattcctta attacttcca gaatcagaat
     6361 tctttttatt ccttttacaa agtacataat cagggtgttc tccagctaaa agcagaagaa
     6421 atttaaactc tactggtgta agtgctgtac cgcttgcagt tccgacaggt tcttctttgc
     6481 gacgccaggt acgcggactc atgctaaata tttcggcaca ttctttctgt gtcaggccaa
     6541 ttttttgccg cagacgttta atgttttctg gtgtgttttc atttggcatt ttttccctca
     6601 ctcatttcct gaatcatcat aaaaataatt t
//
LOCUS       DAZFKF010000187         6619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFKF010000187 DAZFKF010000000
VERSION     DAZFKF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.189"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            475..1503
                     /gene="repA"
                     /locus_tag="ACHKA1_005032"
     CDS             475..1503
                     /gene="repA"
                     /locus_tag="ACHKA1_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFO5682565.1"
                     /translation="MQLAVISKRHWSELSPEEQIRFWQDYEAGIESSFLVPQENKGGT
                     TKRRRGEHSTKPKCENPVWFRPDSYKALGGQLGHAYNRLVKKDPVTGQYTLRMHMSLH
                     PFYVLKRQSVGRKYKFRPEKQRLLDAIWVVLVSFCDRGLHTVGMSVSRLAEEISPKDS
                     KGNVIPETAVTVSRLSRLLAEQVSFGTLGTSEKTIWDRESRQRLPKYVWITETGWKML
                     GVDLVKLQEQQRKRLAESEIRLQLIKEGVIREGEEISVHSARKRWYAQRSLDAIKFRR
                     EKAAKRKRANRLAKLPYDEQRNEIARFILKRMPPDEAYWCTKERLEQLVARDLRQLEL
                     ALTASPPH"
     gene            2737..3006
                     /locus_tag="ACHKA1_005033"
     CDS             2737..3006
                     /locus_tag="ACHKA1_005033"
                     /inference="COORDINATES: protein motif:HMM:NF036604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
                     /protein_id="HFO5682566.1"
                     /translation="MLLALEAFCLMAKRWLYLVVRQKEESPVVNFSLTHEAIPMLEQH
                     QDSLLPRVTQGEEGHETAEKRPDLVFINRVLHIVNIHVSDPESFV"
     gene            2951..3073
                     /locus_tag="ACHKA1_005034"
     CDS             2951..3073
                     /locus_tag="ACHKA1_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5682567.1"
                     /translation="MVCCTLLIFTYLTRNRLCEVRLKDGDREVTASLAYESNGK"
     gene            3223..3819
                     /locus_tag="ACHKA1_005035"
     CDS             3223..3819
                     /locus_tag="ACHKA1_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO5682568.1"
                     /translation="MTAIIEASMQPKKTPVIVVKKRRVLVMPENPVVNEKSQEVQKSA
                     VNENKKVQKKDAVAEKTRKKQPQPWYLKKQITFPPKYPKEYFEKCFNKVRAVFPELWT
                     DEKKNLPLKSGILQDVEKYLADNPDVDLTIEEWNCAVQVMTFRWQYLQNCTVPGATRY
                     DLYGKPAGTVKKAHATYAQLVLDARKKASEKKQLKRKG"
     gene            complement(3837..4121)
                     /locus_tag="ACHKA1_005036"
     CDS             complement(3837..4121)
                     /locus_tag="ACHKA1_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO5682569.1"
                     /translation="MALDLKTPVIPGQKTEQTNADTARFINEATRKKSGATKVTNVRL
                     GDVYEDILEREALRTGQTKTAIMKAALAAYEHGLDENGKNHWILQSARLK"
     gene            complement(4200..4865)
                     /locus_tag="ACHKA1_005037"
     CDS             complement(4200..4865)
                     /locus_tag="ACHKA1_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HFO5682570.1"
                     /translation="MGRALLVASDKGGVGKSTTATNLASYLVNNSRTVIILKTDKNND
                     ILSWNEKRKQNGLLPVPVYEEYGDVSEVIKRLKKICEVLIIDCPGHDSKEFRSALTVA
                     DILLTLVKPSSDFEAETLTHVTEKVRTAQQINPDLQPWVLFTRINTTKPRHRKAAIDL
                     DKLLRENPVWIQPLRTRISDLDIFEAACNEGAGVHDVRRASSLSTAKAQIELLAQELG
                     INP"
     gene            5463..5792
                     /locus_tag="ACHKA1_005038"
     CDS             5463..5792
                     /locus_tag="ACHKA1_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682571.1"
                     /translation="MKTLTFNNGTVSVGDVFVSSWGYEQTNVNFYQVISVHGKKTVTV
                     QEIRASVHRTHSMSGYKTPLLNDFCGEPLKRRVRDYYSKPAIEIESFETAYKGSPEEK
                     HEFTSYY"
     gene            6373..>6619
                     /locus_tag="ACHKA1_005039"
     CDS             6373..>6619
                     /locus_tag="ACHKA1_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020314677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682572.1"
                     /translation="MENHAKFVATEILNQLGGNRFIAMTGAKNFACFDENGECGLCFR
                     LPSNFAMKGINLVKIKLTFSDTYLVTFSRVRGATVKEI"
BASE COUNT         1779 a         1490 c         1583 g         1767 t
ORIGIN      
        1 tttgtgtggt ttattcgatc gtgtgagtgg gtgtatgcag tcaaaaaaac ttttttgaat
       61 atacaaaatt ctgaatttct gattttgtaa ggttttgtat gaggaacgtc agtaaagaag
      121 gggcttcagt agctgttctg aaaacgattt tgaagctgtc agagatgtgt gctgaatcaa
      181 aaaggaaacg agccaggctt gaacatcgtt gatcgattgc gcccatgctg ctaaatttgc
      241 aggcaagttt tccgcgattt gtaaatgcag aaaacgaggt gctgaaattt tgttcaagaa
      301 cgtaaatttc agataaagaa aacccccgta atctcgtgtt ttccaggcag cgtagattag
      361 cgggggttgc cctcaaggcc gagccttgag cttatttatt tcaggtatgc ttgttacctg
      421 aaatggagtt taattccatg agaaggaaat atcaactctt acagtgcaag ctaagtgcag
      481 cttgctgtga ttagcaaacg tcactggtct gaactatcac cggaagagca gatccgtttc
      541 tggcaggact acgaagcggg aattgaaagc tcattcctgg tcccccagga aaacaaaggc
      601 ggaacaacaa aacgtcgccg gggtgaacat tccactaagc caaagtgtga gaatccagtc
      661 tggttccgtc ccgacagcta taaggcgctg ggcggacagt tgggccacgc ttacaatcgt
      721 ctcgttaaaa aagacccggt cactggtcag tacacactca gaatgcatat gtcgttgcat
      781 ccattttatg tactgaaacg tcagagtgtc ggtcgcaaat ataaattccg accggagaaa
      841 cagcggctgc ttgacgcaat atgggttgta cttgtgagtt tttgtgatcg tggattgcac
      901 actgtcggca tgtctgtctc ccgcctggca gaagaaataa gcccaaaaga cagtaaggga
      961 aacgttattc ctgaaaccgc agtcacagta tcgcgccttt cccgtctgct ggcagagcag
     1021 gtttcctttg gcacgttggg aacgtcagaa aagacgatat gggaccgtga atcccgccag
     1081 cgactgccga aatacgtctg gatcaccgaa actggctgga aaatgctggg agttgatctg
     1141 gtgaaacttc aggaacagca acgtaagcgc cttgcagaaa gtgaaatccg tctgcaactg
     1201 attaaagaag gcgttatccg cgagggcgag gaaatctctg ttcactcggc tcgtaaacgc
     1261 tggtatgcgc agcgttctct cgatgccatc aagttccgac gagagaaagc cgcaaagcgc
     1321 aaacgtgcca atcgccttgc aaaactgccg tatgatgaac agcgaaacga aattgcacgg
     1381 tttattctga agcgtatgcc gccggacgaa gcgtactggt gcactaaaga aagattagag
     1441 caactggtag ccagggatct acgtcagctt gaactggccc tgacagcttc gccaccccac
     1501 tagtttagct gcctgataca atcggcactc tcttcagccc cttcggggct tttcgtgttg
     1561 cctgcatttc atgaaattcc tcatttttga cgaccgtttt tcattttgtc ccgctctgac
     1621 agttttttga tggccacaat cgggtatcgg agctaaaccg ccttcggtta ttcacaaaaa
     1681 cccgcaaacg aataacttca gcaagcagca aacgtagaag atcaaacccg caatatattc
     1741 ttaacccctg ttctttaatc ccctgcgttg cttcgccgca gggaaaatat ttatctttga
     1801 aacaactgtg gataatcaca aaaatgcctt cgcttgcagc ggctaaagcc gcgccgctca
     1861 gaatataaaa gtacctccca ccgcttcgcg gcgggcatat gaacttctgc ccgatgaaaa
     1921 acaaagcccc tccaaccctc ctacaaaaaa acgctgcaaa tactcagcca cagcgcccaa
     1981 cccatcactg aaaagcgccg ccgcccccgc ccaaagggcg ggaacaacat cgctttcaat
     2041 aatggatgtt gtaactaaga aattacatgg ctgtcagtct tctggctgga agtaccgagt
     2101 acacgctcgt aagcggccct aacggcccgc taacgcggag atacgccccg actgcgggta
     2161 aacccttgtc gggaccactc cgaccgcgca caaaagctat ttcatggctg aaagcgggta
     2221 tggcttagca ggatggggat gggtaaggtg aaacctatca atcagtaccg gcttacgccg
     2281 ggcttcggcg gttttaatcc agtatcatat gtaacaacgg agtgccgcct tccatgccgc
     2341 tggcgcggca tcaaaaaaga aagcccccca atgacggggg gaaatagcgt tttgatcaaa
     2401 ataataatgc tagtgtgctt cttgcgtgcc attccaggca caaactaaat ttaacgtttc
     2461 attaactaca aagaaatccg atgtgttaat cacttgcgaa ataaaaatca tatagtaaat
     2521 tactatatga ttttgcgttg tttcggagaa ttaaaaaatg cctttgattg aatacatcaa
     2581 caagttctat cgggggaatc aggcatcgtt cgccaggctg accggagttc aacctgcgca
     2641 ggtcacacag tggatcaaca aaggattcat cgtggtgaat cacacactgt atagtccacg
     2701 ccgaaagctg ggaatttaat tcctggtcga tatataatgc tgttggcgct tgaggctttc
     2761 tgcctcatgg cgaaaaggtg gttgtatctt gttgtgcggc agaaagaaga aagccccgta
     2821 gttaattttt cattaaccca cgaggcgatc ccaatgcttg aacaacatca ggatagcctc
     2881 ttaccgcgcg ttacgcaagg agaagaaggc catgaaacag ccgaaaaacg ccctgacctg
     2941 gtgtttatta atcgtgtgct gcacattgtt aatattcacg tatctgaccc ggaatcgttt
     3001 gtgtgaggtc cggctgaaag acggagacag ggaggttacg gcaagtctgg cttacgaatc
     3061 caacggtaag tagcaacccg gaggcgggcg aaagcccgcc tttttgggga tgatgtggtt
     3121 ctggcattaa gcgcctttgc aaagggggta ttctgtacgg ataccccctt tttccatgta
     3181 ttttcaggat attaactgct gaaaatggta taattagtca atatgacagc aattattgag
     3241 gcgagtatgc agccaaaaaa aacacccgtg attgtggtca aaaaacgccg cgttctggtc
     3301 atgccagaaa atcctgtggt gaatgaaaag tcgcaggaag tacaaaaatc agcagttaat
     3361 gaaaacaaga aagtacagaa gaaagatgct gtagcagaga aaacacgtaa aaagcagcct
     3421 cagccctggt atttgaaaaa acaaatcact tttcccccaa aatatccgaa agaatatttt
     3481 gaaaagtgtt tcaataaagt tcgtgctgtt tttcctgaac tatggacaga cgaaaaaaag
     3541 aacttgcccc tgaaaagcgg gattctccag gacgttgaga aatacctggc ggataacccg
     3601 gatgtggatc tgacgattga agagtggaat tgtgcggttc aggtgatgac gttccggtgg
     3661 caatatcttc agaattgtac tgtaccaggc gcaacacgtt acgaccttta cgggaaacct
     3721 gctggtacag taaaaaaagc acacgcaact tatgcgcaac tggttctcga tgcccgtaag
     3781 aaagccagcg agaagaaaca attaaaacgt aagggataaa gccccgaagg gctttatcat
     3841 ttcagcctgg ctgactgtag aatccagtga ttttttccgt tttcgtccag gccgtgttcg
     3901 taagctgcaa gagccgcttt cattattgct gtttttgtct ggcctgttcg cagtgcttcc
     3961 cgttccagaa tatcttcgta aacatcccca agacggacat tggtcacttt agttgctccc
     4021 gattttttgc gggtagcttc attgatgaat cgggctgtgt cagcattggt ttgttctgtt
     4081 ttttgtcctg gtattaccgg agttttcaag tcaagagcca ttatatcacc tttcgtgtgt
     4141 tacattaacc ttacattgta aagctaatga tgtaaagcta tcgtattaca tccattaact
     4201 cagggattga tgcctaactc ttgcgccaaa agctctattt gtgctttagc tgtagaaagg
     4261 ctggatgcgc gacgtacatc atgaacgcca gcgccttcat tacaggccgc ttcaaaaata
     4321 tctaaatctg aaatacgtgt acgaagtggc tgtatccaca ccgggttctc ccggagtaac
     4381 ttgtcgagat caatcgctgc ttttctgtgg cgtggttttg tcgtgttaat tctcgtgaaa
     4441 agtacccagg gttgcaggtc tggattgatc tgttgagctg tgcgaacttt ttctgttaca
     4501 tgagtcagcg tttctgcttc gaaatctgaa gacggcttaa cgagagtaag cagaatgtca
     4561 gctacagtta gggcactgcg aaattccttg ctgtcatgcc ctggacagtc aatgatcagc
     4621 acttcacata tttttttcag gcgcttaatg acttctgata catcgccata ctcttcataa
     4681 acaggaaccg gtaataagcc attctgcttt cttttttcgt tccagctaag aatatcattg
     4741 tttttatcgg tcttcagaat gatgaccgtt cgtgaattat ttaccagata agaggccagg
     4801 tttgttgctg tggtgctttt tcctacaccg cctttatctg atgcgacaag taatgctctt
     4861 cccatagaaa cctcggattt acagtgtaaa ggtttatttg tctttacatt gtattcatca
     4921 tgattacgtt gtaaagatgt ttgtgtcttt acgttgtaat tatcgtttgt gctttacatt
     4981 gtgtgtgtgt tacagggtaa cttttcagga tgtgatttcg cctggtgtgt tgttgcgccc
     5041 gtagctcgct gacgctcaca ctcgcagtac agcgccccgc cctgctgcaa tgcaccagga
     5101 cggcgtaaac tcgctggcgc tcgcgctaac tgcggcattc tgcggcgcaa atcagcatga
     5161 tcatggcctg tcagtccgtt gggctttgca gggtgaaggg atggccctgt gcatcctggt
     5221 atgttcgtca gcggctgcgc cgcgcttcgg ggcgatgtgg ccccgctgca ccctggtatt
     5281 ttctttcgcc ccttttatgc cctgccgggc attatatgaa tgtaactatc tgtatttgat
     5341 ggtatttatt ttaaaggaaa gaggaaaaaa tacttgataa taggtcagtt ctgacctatt
     5401 ataaacacat cgggagggga tggcccccaa tcaccagagc cgctcagcgg caggagataa
     5461 aaatgaaaac attaactttt aataacggta ctgtttctgt tggcgatgtg tttgtatctt
     5521 cctggggata tgagcaaacg aacgttaatt tctaccaggt tatttctgtt catggcaaaa
     5581 aaactgtaac tgttcaggag attcgcgctt cagttcatcg gacccattca atgagcggat
     5641 ataagacccc attacttaat gatttctgtg gtgagccatt aaaacggcga gtgcgtgatt
     5701 attacagtaa gccagcaatt gaaattgagt cattcgaaac agcatataaa ggatcaccgg
     5761 aagaaaaaca cgagttcaca tcatactact aagaaaatag ttgcaggggg tatcccctgc
     5821 aaccatgtta aattgttaat agtgaataca ggggcttaat cgcccctttt ttatttggtg
     5881 cgcagtaaaa ctccgtcctt caaggcgtgg agaatgccag gcgaatgcag caatgtaatg
     5941 ttgcgcccgt agctcgctga cgctcacacg cgcagtacag cgccccgccc tgctgcaatg
     6001 caccaggacg gcgtaaactc gctggcgctc gcgctaactg cggcattctg cggcgcaaat
     6061 cagcatgatc ataggctgtt ggtctgtcct ggctttgcag ggcgaaggga tggccctgtg
     6121 catcctggta tgttcgtcag cggctgcgcc gcgcttcggg gcgatgtggc cccgctgcac
     6181 cctggtattt tctttcgccc cttctatgcc ctgccgggca ttgcatgagt ataactatat
     6241 gtatttgatg gtatttattt taaagaaagg agaaaaaaat acttgataat aggtcagttc
     6301 tgacctatta taaacacatc gagaggggat ggtccccaat caccaaagcc gctcagcggc
     6361 aggagcttaa aaatggaaaa ccacgccaaa tttgttgcta ctgaaattct taatcagctt
     6421 ggtggaaacc gctttattgc tatgacaggc gcaaagaact ttgcctgttt tgatgaaaat
     6481 ggtgagtgtg gtctttgctt tcgtttgcct tcaaattttg caatgaaggg aattaatttg
     6541 gttaaaatca aattaacctt ctctgataca tacctggtga cattttcccg tgtccgtgga
     6601 gctacagtaa aagaaatat
//
LOCUS       DAZFKF010000188         6551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFKF010000188 DAZFKF010000000
VERSION     DAZFKF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.190"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(346..771)
                     /gene="nudI"
                     /locus_tag="ACHKA1_005040"
     CDS             complement(346..771)
                     /gene="nudI"
                     /locus_tag="ACHKA1_005040"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO5682573.1"
                     /translation="MRQRVIVCPVIQNEGEFLLCKMASDRGVFPGQWALPGGGMEPGE
                     TMETALRREIKEELGKELHITDVKPWAFRDDIRTKTYPDGTHEDIYMIYLIFDCISTN
                     RTVTFNEEFQDIVWASPDTLKYMDLNEATRITFSQKGIL"
     gene            complement(773..1480)
                     /locus_tag="ACHKA1_005041"
     CDS             complement(773..1480)
                     /locus_tag="ACHKA1_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014727861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFO5682574.1"
                     /translation="MRYNYLYMDPWSADKKEKNKTRNPLYICLSGNSGVGKSTLLRRM
                     SKELFKVDPDTVAIDEKSVHHTLLPYLFDDTSNYGYLIQLNFMIQRALLIKSWLDKGF
                     NLIMERSHIEDYIFINFMLKSEYINKAQYDAYMVLWSRINELLTEPDIIAFINYPIAH
                     SLMHLNDDEIHGVRPEEFPDEEMKIKWITGWHAEYQHFTDNLPLHLRERIMVCAHPDD
                     IDDFSDKVIQKAMMLRT"
     gene            complement(1477..2343)
                     /locus_tag="ACHKA1_005042"
     CDS             complement(1477..2343)
                     /locus_tag="ACHKA1_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO5682575.1"
                     /translation="MDISTLLLDFRITSHCNLGCDLCFRTPGIQDSSLASARNVIERM
                     YKMGFRRIGFTGGEPTSRSDFIDLIEYAKQLGFMTYLSTVGHRFIMDHARLNPVLDWV
                     GIPIDGIDSLVNSEIRSDKMSNQHRVIKNILTWLPQNNNRINIKLTTVVSQSNINHLS
                     DIVCWVDSLPYKIQAWRFYQFCPLGVGKEKRDKLEINTDIFKDKMVMLKKQHPNMPIS
                     WATFEERDKANVVMEPNFDIIIPDGENYTLLGNMLTDSQDHIISAIFGNNEIMQKCKN
                     NRFWLDKMEFTV"
     gene            2828..3079
                     /locus_tag="ACHKA1_005043"
     CDS             2828..3079
                     /locus_tag="ACHKA1_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO5682576.1"
                     /translation="MPNIILSDISASVSELKKNPMATVGAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSSQPLHDVDLDSYS"
     gene            3076..3363
                     /locus_tag="ACHKA1_005044"
     CDS             3076..3363
                     /locus_tag="ACHKA1_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5682577.1"
                     /translation="MTYTIKFRDDALKEWLKLDKTIQQQFVKKLKKCSKNPHILSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYYLASERMR"
     gene            3531..3935
                     /locus_tag="ACHKA1_005045"
     CDS             3531..3935
                     /locus_tag="ACHKA1_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFO5682578.1"
                     /translation="MTILVTAQAQLIVGEAMAIKSLAPDGMLAAVFEDDGNTGYFYAL
                     DESVEGNPVRDAVHIYNVEDISDAHIPSDVKIGWSEDCLKCVLLINGYPHGVFDFEGK
                     NGYCRSGFPPPVNHEWSVQGHSWDDYVDNFFR"
     gene            4244..5728
                     /locus_tag="ACHKA1_005046"
     CDS             4244..5728
                     /locus_tag="ACHKA1_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5682579.1"
                     /translation="MRKIIAHFKIVLTLLLPATVSAQSIEWQSCMTTPFSNWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDSSSVKRQQVKLALTKLPAKSRHKGSIIIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSIPAINCQQSDTGIQENITEKQRVLNKI
                     NSCIHNTGTEVIRHIGSNEAVYDIDRIRQALGDEQLTAVAYSYGTQIAALYAERFPSS
                     IRSIVLDGVVDIDDLGDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSVRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELILLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSTPIASEESSDALGVILCVDQGGEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSNLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVRHLITPMKMGNISCPGNAEIDIK"
     gene            complement(5949..>6551)
                     /locus_tag="ACHKA1_005047"
     CDS             complement(5949..>6551)
                     /locus_tag="ACHKA1_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO5682580.1"
                     /translation="KDLEEKKNPVIVVKKRRSFSPPSLAEKKDVIAPALTEQATESVS
                     SGISSPAVETHIPEAPARKKKKKRQRFPRPSHWTSEFTHDCLEKVKALFPQLRAEGGG
                     FLPLKTGITRDITTYLAAHPETALTLDEWNCAVSCITSRRIYLQRTSVAGTPRYNLDG
                     QPEGEVSECDAQNSRRWLAAKEQRWLSMKARQENISEKSK"
BASE COUNT         1867 a         1364 c         1391 g         1929 t
ORIGIN      
        1 tccttcatca ttaatgctaa tgtatgcata cattagcatt aatgatggtg cgttgaacgt
       61 acaataaggg gtccgcttgg aattcggaaa ttttcccacg ctaagagaat tgagttgaac
      121 gagtggttac atccaggcca gctaactcac tgaacgtaaa gactgtacga aatgttccga
      181 ccgtccgata accatttggc ttacttccgt acatttcctt gcgtagatgt acagaattgt
      241 acgttttcgt acggttggcc ctcaaacccc atccttgagt agtggcataa gaaaacggct
      301 ccctgttgtg gaagccgtta tcagttgcac gattagcatt ggagtttaaa ggataccttt
      361 ctgagaaaat gtgatccggg ttgcttcatt taaatccata tattttaggg tgtcaggaga
      421 tgcccagact atatcctgaa actcctcatt aaatgtaact gtacggtttg tactgataca
      481 gtcgaatatt aggtaaatca tgtaaatgtc ctcgtgggta ccatccgggt acgtttttgt
      541 acggatatca tcacgaaagg cccagggttt tacgtcagtg atatgcagtt cttttcccag
      601 ttcttccttt atttcacgcc tcaatgctgt ttccatagtc tctccgggtt ccattcctcc
      661 accgggcagt gcccattggc ccgggaatac tccgcgatca gaggccattt tacaaagtag
      721 aaactcacct tcattttgta tcacagggca cactattact ctctggcgca ttttatgtcc
      781 tcaacatcat ggctttttgg ataaccttat cagaaaaatc atcaatgtca tccgggtgag
      841 cacataccat aatccgctca cgaaggtgta acggaagatt atcagtaaaa tgttgatatt
      901 ccgcatgcca tcctgtgatc cattttatct tcatttcctc atctggaaat tcttcaggcc
      961 gtacaccatg aatttcatca tcattgagat gcatgagtga atgtgcgatg ggataattaa
     1021 tgaatgctat aatgtctggc tctgtaagta attcatttat tctactccag agcaccatgt
     1081 atgcatcata ttgtgcttta tttatatatt cggacttcag catgaagttg ataaaaatat
     1141 aatcctcaat atgggatctc tccataataa ggttaaaccc tttatccagc caagatttta
     1201 tcagtaatgc tcgttgtatc ataaaattaa gctggattag ataaccgtag ttactggtat
     1261 cgtcaaaaag gtaaggaagg agagtgtggt ggacactttt ttcatctatc gcgacagtgt
     1321 ctggatctac cttaaataac tcctttgaca tgcgtctcag aagagtactt ttacctacac
     1381 cggaattacc cgacagacat atatacagag gatttcgagt tttattcttc tcttttttat
     1441 ctgctgacca aggatccata tacaagtaat tatatctcat actgtaaact ccattttgtc
     1501 caaccaaaag cggttattct tacatttttg catgatttca ttatttccga aaattgcact
     1561 gattatatga tcttgtgaat ctgtcagcat atttcccaac agagtatagt tttctccatc
     1621 agggataata atgtcaaaat tgggttccat aacaacattg gctttgtccc gttcttcgaa
     1681 agttgcccaa ctaatgggca tgttagggtg ttgttttttt agcattacca ttttatcttt
     1741 aaatatatcc gtattgattt ccagtttatc tcttttttct tttccgactc ctagaggaca
     1801 gaactggtaa aatcgccaag cttggatctt atagggcaga gaatcaaccc agcaaacaat
     1861 atcactgaga tgattaatgt tagactgaga tacgacggtc gtgagtttta tattaattcg
     1921 attattattt tggggtaacc atgttaatat atttttgata accctgtgtt ggttactcat
     1981 tttatcactg cgaatttctg aattgacaag actgtcaatt ccatcaattg gtattcctac
     2041 ccagtctaac accggattta accgagcatg atccataata aatcgatgcc cgaccgtgga
     2101 gagatatgtc ataaaaccca gttgcttagc gtattcaatc agatcaatga aatcactccg
     2161 ggatgtaggt tcccctccgg taaaaccgat acgacggaat cccattttat acatccgctc
     2221 aattacatta cgagcactag caagagaaga gtcctgaatt ccaggtgtac gaaaacacag
     2281 atcacatcct aggttacaat gtgaagttat tctgaaatct aaaagaaggg tgctaatatc
     2341 cataacaagt cctctgacta tgatacatca ataaccatta aaacataatg gttattgcag
     2401 accatgacaa agcgcaacct tcggcttacc agggatataa tatctgccag taatcagagc
     2461 agtgggttgc gcaaggaact catgaatgat gagttccggt aatcgcatta ctggaagccc
     2521 atgatcggag ggacgctatc agtcacacca gtgaaaattg tgcacagaag cattttcatc
     2581 actgacaggc cgtatgcctc acaagacggg agataactgg agaatattta gttcttctac
     2641 cggcttacgc cgattttact cctgtgttat atgtaaccat tgtgtgccac cttccatgcg
     2701 ctggtgcggc atcagagtaa aaaatcagca ttaaatttat ttgcaaatgt atgagtaata
     2761 cagacttgtt aacgggtcct ttcagatgta tctttaagac ttattaaaca gtcttaaaag
     2821 gtgcattatg cctaacatca ttctgtctga catcagtgca agtgtcagtg aactgaaaaa
     2881 aaatccgatg gcgacagtcg gtgctggtga tggttatccg gtcgccattc tgaatcgcaa
     2941 tcagccggca ttctactgtg ttcctgctga actgtatgaa aggatgcttg atgctctgga
     3001 tgaccaggag ctggtgaaac tggttactga acgtagcagt caacctctgc atgatgttga
     3061 cctggacagt tattcatgac ttatacgata aaattcaggg atgatgcgct taaagaatgg
     3121 ctgaaactgg ataagaccat tcaacaacag tttgtgaaaa aactaaaaaa atgtagcaaa
     3181 aatcctcata tactgtctgc aaaactacgt gggctaaagg actgctacaa aattaaactt
     3241 cgtgcatcgg gatttcgtct ggtttatcag gtgattgatg atatgttaat tattgcggtt
     3301 gttgcggtag gtaaacgaga acgtagtaac gtttattatc tcgccagtga gagaatgaga
     3361 tagtaacata aatagccttt cccgcagata actctcagaa ttgttattct ctctgtggcc
     3421 tcgcctgagg cggaaaaaga aaagttttgt tgagagaatg attttacctg ccgggatgag
     3481 tctggggtta tttatattac cagacaaatt gaaacggagg gacatgtttg atgacaatac
     3541 ttgtgacggc acaggctcaa ctcatagttg gtgaggctat ggcgataaaa agtttagctc
     3601 ctgatggaat gttggccgct gtatttgaag acgacgggaa taccggatat ttctatgcac
     3661 ttgatgagtc cgttgaaggt aaccctgtca gggatgctgt gcatatatat aacgttgaag
     3721 acatttcaga tgctcacatc ccctctgatg tgaagattgg ctggtctgag gattgcctga
     3781 agtgtgttct gctgataaac ggatatccac atggggtatt cgattttgag gggaagaacg
     3841 gctactgtcg aagtggattc ccgccaccgg ttaatcatga atggtctgtt caggggcatt
     3901 catgggatga ttacgtcgat aatttcttcc ggtgattgaa ccgaaaaatt acctgacgga
     3961 caagtctggg gccgaatcgt gatgattcat catggccgtt tatagttcag cagtcttgtt
     4021 cttttgctgt cagtgggata ccaggaaaat aaccgattgt taccagacag aataaccatc
     4081 ataagacgtc acaagacggt gacgcttaca tagttccggg gagttaaaca atataccctg
     4141 actttgtaat catttgaatt atatccgtaa cgtgtaactt cctgccgtaa caccggattt
     4201 aagatattca aactttatct ttataccgat attaagagac acaatgcgaa aaataattgc
     4261 tcatttcaag atagttttaa ccttacttct gcccgcaaca gtatctgccc agtcgataga
     4321 gtggcaatcc tgtatgacca ctccattcag taactggttt ggtgaggaaa aaccgtctcc
     4381 tgacttacta tgtggttatt tgtctgttcc attaaaatat acagacacag gcggagatag
     4441 ttcttctgta aaaagacagc aggtaaaact ggcgctgaca aaattgccag caaaaagcag
     4501 gcataaaggg agtatcatta taataagtgg tggaccagga ttacctggca ttaatcctta
     4561 tattaacttt gactggccgg taacaaatct tcgcgaatca tgggatatta ttggctttga
     4621 tccacgggga gtcggccagt ccattcctgc aataaactgc cagcaatcag atacagggat
     4681 tcaggaaaac ataactgaaa agcaacgggt attaaataaa attaacagct gtatccataa
     4741 taccggaact gaagttattc gccatattgg ttctaacgag gcggtatatg atattgatcg
     4801 tatcaggcaa gccctgggtg acgagcaact gacagctgtg gcatattcgt atggaacaca
     4861 aattgcagcc ttatatgcag aacgtttccc atccagcatc agatctatcg ttcttgatgg
     4921 ggtcgtcgat attgatgacc tgggggacaa cttcacatgg caactcaaac aagcacagag
     4981 ttatcaggaa acgttcgatc gctttgcatc ctggtgtgca cgtacaaaaa gttgcccact
     5041 ttcttcagtc agagataagg caataactca gttccatgag ctgttatcaa aattgcatca
     5101 caagccttta ttagacagca agggagaaaa tatatcttca gatgaactca tattattaac
     5161 aacagacctt ttgttatggc gttcatcatg gccaaccctt gcaactgcca tacgtcagtt
     5221 ctctcagggg attgtcagta atgaaattga aactgcgctc agtactccta tagcctcaga
     5281 agagtcaagt gatgctctgg gggtaatcct ttgtgttgat cagggggggg agcaattaac
     5341 cccagaagag cgaaaatccc gaaaagatgc tcttgcgaat gccttcccgg ctattaactt
     5401 tgacaatgga cgttccgatt cacctgattt ttgtgaatta tggccaatac atagcaacct
     5461 gaataaaacc cggctgaaaa atacagttct gccctctggt ttactgtttg tagcacacaa
     5521 atacgatcca acaacaccat ggattaatgc tcgtaagatg gcagagaaat tttccagccc
     5581 gttgctgaca ataaatggtg atgggcatac attagctctt accggagtca atttatgtgt
     5641 agataaggcc gttgttcgtc atcttatcac ccctatgaaa atgggaaata tatcctgtcc
     5701 aggaaatgct gaaatagata taaaatagtt taaaaattgc ccgccatttg ctcctgatac
     5761 cggacatatt ttgcattggg agcaaaatgc gttcagtgcg atcacggcac cagaaacgtc
     5821 aaactggata cacgtaaaaa atgtcgcgat cgcgcaaaat caagccatta ctctcgggag
     5881 tgcttaaata atacgcacaa tatacaacgc tctcttctct acagtctgtt tcctgtaata
     5941 tcctgcggtt attttgattt ctcagaaata ttttcctgcc gggccttcat actcagccac
     6001 ctctgctctt tcgccgccag ccagcgcctg gaattttgag catcacattc cgatacttcc
     6061 ccctctggtt gaccatccag gttataacgg ggagttcctg caacagaggt tctctgcagg
     6121 tagatccggc gactggtaat gcaggatact gcacaattcc attcgtccag ggtcagcgct
     6181 gtttccggat gtgctgccag atatgtagta atgtcacggg ttatgcctgt tttcagaggc
     6241 agaaaaccgc ccccttcggc acgaagttga ggaaacagcg ctttcacctt ctccagacaa
     6301 tcgtgagtga actcactggt ccagtgggat ggtcttggaa agcgttgtct tttctttttc
     6361 ttcttcctgg ctggcgcttc ggggatatga gtttcgacgg caggtgaact gattccggag
     6421 gagactgatt ctgtcgcctg ttcagtgagt gcaggcgcga taacatcttt tttttcggct
     6481 aaggaaggcg gtgaaaaaga gcgacgtttt ttcaccacaa tgacgggatt tttcttttct
     6541 tccagatctt t
//
LOCUS       DAZFKF010000189         6451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFKF010000189 DAZFKF010000000
VERSION     DAZFKF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.191"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            116..1396
                     /gene="wcaK"
                     /locus_tag="ACHKA1_005048"
     CDS             116..1396
                     /gene="wcaK"
                     /locus_tag="ACHKA1_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO5682581.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FHDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAHQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSCERQTGMQMVQSVLERIGEVK"
     gene            1393..2613
                     /gene="wcaL"
                     /locus_tag="ACHKA1_005049"
     CDS             1393..2613
                     /gene="wcaL"
                     /locus_tag="ACHKA1_005049"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO5682582.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVDIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVVTPLHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISNLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            2624..4018
                     /gene="wcaM"
                     /locus_tag="ACHKA1_005050"
     CDS             2624..4018
                     /gene="wcaM"
                     /locus_tag="ACHKA1_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO5682583.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAASFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            4176..5171
                     /locus_tag="ACHKA1_005051"
     CDS             4176..5171
                     /locus_tag="ACHKA1_005051"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFO5682584.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKSPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            5414..6301
                     /gene="galF"
                     /locus_tag="ACHKA1_005052"
     CDS             5414..6301
                     /gene="galF"
                     /locus_tag="ACHKA1_005052"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO5682585.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGIKFREKILKLL"
BASE COUNT         1717 a         1586 c         1620 g         1528 t
ORIGIN      
        1 gttctgtgct cgggtcttgt aggcctgata agacgcgcca gcgtcgcatc aggcaaaaaa
       61 cggcaacgaa cacaacaaac acagcctcac ctggcatcaa atttatgagg aaaaaatgaa
      121 attacttatt ctgggcaacc acacttgcgg caatcgtggc gacagcgcca tcctgcgcgg
      181 cttacttgat gccatcaaca tcctcaatcc acacgccgaa gtggacgtca tgagccgcta
      241 tccggtcagt tcttcctggc tgctcaaccg cccggtaatg ggcgacccgc tgttcctgca
      301 aatgaaacaa cacaacagcg cggcgggcgt tgtcgggcgc gttaaaaaag tcctccgtcg
      361 ccgctaccag catcaggtat tgctctcacg cgtcaccgac actggcaagc tgcgtaatat
      421 cgccatcgcc cagggattca ccgacttcgt gcgcctgctg tcaggttacg acgccattat
      481 ccaggtcggc ggatcgtttt ttgtcgatct ctacggcgtg ccgcagtttg aacatgcgct
      541 ttgcacattt atggcgaaaa agccgctgtt tatgattggt cacagcgtcg gcccgttcca
      601 tgatgagcaa tttaaccaac tggcgaacta cgttttcggt cactgtgacg cgctgatcct
      661 gcgcgaatcg gtcagcctcg atctaatgaa acgcagcaat atcactaccg caaaagtgga
      721 acatggcgtc gatactgcgt ggctggtcga tcaccacacc gaagatttca ccgccagcta
      781 tgccgtccaa cactggctgg acgttgccgc acaccagaaa acggtggcaa ttaccctgcg
      841 cgaactggca ccgttcgaca aacgcctcgg caccactcag caagcgtatg aaaaagcctt
      901 tgccggggtg gtcaatcgca ttctcgacga aggctatcag gtcattgcgc tctccacctg
      961 tacgggtatc gacagctata acaaagacga ccgcatggtg gcgctcaacc tgcgccagca
     1021 catcagcgat cctgcccgtt accacgtagt gatggatgaa ctcaacgatc tggaaatggg
     1081 caaaattctc ggtgcctgtg aactcaccgt cggtacgcgt ctgcactccg ctattatctc
     1141 aatgaacttt gctactccgg cgattgccat caactatgaa cataaatccg ccgggatcat
     1201 gcaacaactg gggctgccag agatggcgat tgatatccgt catttactgg acggcagctt
     1261 gcaagcgatg gttgcggata ccttaggcca acttccggcg ctgaacgcgc gacttaacga
     1321 agccgtcagt tgcgaacgtc agacgggaat gcagatggtg cagtctgtac tggaacgcat
     1381 cggggaggtg aaatgaaggt cggcttcttc ttattgaaat ttccgctgtc gtcggaaacc
     1441 ttcgtcctca atcaaattac cgcgtttatt gatatgggat ttgaggtgga tattgttgcg
     1501 ctgcaaaaag gcgacacaca aaacacccac gcggcatgga cgaaatataa ccttgccgcc
     1561 agaacccgct ggttacagga cgaacctacg ggcaaagtgg cgaaactgcg ccaccgcgcc
     1621 agccagacgt tacgcggcat tcatcgtaaa aatacctggc aggcgctcaa cctcaaacgc
     1681 tatggtgccg agtcgcggaa cctgattttg tctgccattt gcggccaggt cgtaacaccg
     1741 cttcatgccg atgtctttat cgctcacttt ggtccagcag gggtaaccgc agcaaaactc
     1801 cgcgaactgg gtgtcattcg cggcaaaatt gctactatct tccacggcat tgatatctca
     1861 agtcgggaag tgctcaacca ctacaccccc gaatatcaac aactgtttcg ccgtggcgac
     1921 ctgatgttac cgataagtaa tctgtgggcc ggaaggctgc aaaaaatggg ctgcccgagg
     1981 gaaaaaatcg ccgtatcgcg tatgggtgtg gacatgacgc gctttagccc gcgtccggtg
     2041 aaagcgcccg caacaccgct ggaaatcatt tccgtcgcac gcttaaccga gaaaaaaggc
     2101 ctgcatgtgg cgatcgaagc ctgccgtcag ttgaaagagc agggcgtggc atttcgctat
     2161 cgcatccttg gcatcggccc gtgggaacga cgcctgcgca ccctcatcga acaatatcaa
     2221 ctggaagatg tggtagagat gccgggcttt aaaccgagcc acgaagtgaa agcgatgctc
     2281 gacgacgcgg atgtcttcct gttgccatcg gtaacgggcg cggatggcga tatggaaggc
     2341 attccggtag cgctgatgga ggcgatggcg gtcggcattc cggtggtttc tactctgcat
     2401 agcggaatac cggaactggt ggaggctgac aaatccggct ggctggtgcc tgagaacgat
     2461 gctcgcgcac tggcgcaacg actggcggcg tttagccaac tggacaccga cgaactggct
     2521 ccggtcgtca aacgtgcgcg cgaaaaagtc gaacatgatt ttaaccagca ggtgatcaat
     2581 cgagaactcg caagcttgct gcaagcttta tagaggagaa cgcatgccat ttaaaaaact
     2641 ctcccgacgc accttcctga cggcaagctc ggcgcttgcc ttcctccata cccctttcgc
     2701 tcgcgcactt cccgcccgac aaagcgttaa cattaacgac tacaacccac acgactggat
     2761 cgcctcattt aaacaagcct tcagcgaagg gcaaacggtc gtcgtgcctg ctggattcgt
     2821 ttgtgacaat atcaacaccg gcatcttcat tcctcctggc aaaacgttac acatccttgg
     2881 aagcctgcgc ggcaacggca gagggcgatt tgtcttacag gacggcagcc aggtgacagg
     2941 ggaggagggc ggcagtatgc ataacatcac cctggatgtg cgtggttctg actgcaccat
     3001 caaagggctg gcgatgagcg gctttggtcc ggtaacgcag atttatatcg gcggcaaaaa
     3061 caaacgggtc atgcgcaacc tgaccatcga taacctgacc gttagccacg ctaattacgc
     3121 catcttacgc cagggatttc ataaccagat tatcggtgcc aacatcacca attgtaagtt
     3181 cagcgactta cagggcgacg ccattgaatg gaacgtggca attaacgaca gtgatatttt
     3241 gatctccgac catgtcatcg agcgcatcaa ctgtactaac ggcaaaatca actggggcat
     3301 tggcataggc cttgcgggaa gcacttacga taataattac ccggaagatc aggcagtgaa
     3361 aaactttgtc gtggcgaata tcacgggatc ggattgtcgg cagttgatcc atgttgaaaa
     3421 tggcaaacat tttgttatcc gtaatatcaa agcccgcaat atcacgccgg atttcagtaa
     3481 gaaagcgggc attgataacg cgacagtcgc tatttacggt tgtgacaatt tcgtgattga
     3541 taatattgaa atgattaata gcgccgggat gttaatcggc tatggggtaa ttaaaggcaa
     3601 atatctctcg ataccgcaaa atttccgagt gaataatatt caactggata acacccatct
     3661 tgcttataaa ttgcgcggca tccaaatctc cgccgggaat gctgcctcct ttgtggcact
     3721 gactaacatt gagatgaagc gtgcctcgct ggagcttcac aataaaccgc aacatctttt
     3781 tatgcgtaat atcaaagtga tgcaggaatc ctcagttggt cccgcattga gcatgaactt
     3841 cgacatgcgc aaagacgttc gcggcgtgtt tatggcgaaa aaagaaacac tgctgtctct
     3901 tgcaaatgtt catgcggtga atgaaagagg gcaaagctcc gtcgatatcg acaggattaa
     3961 tcaccatatt gttaatgtgg aaaagattaa ctttagattg ccggaacgga gagagtagat
     4021 ttgcgaccat tcataaactt actggatata atgttctaaa agattataat cctcacacta
     4081 actgttaaag ctggggctgg gcagcattga ctactcaggg aaggtactca tctttatctt
     4141 gtgtttctta cgtaacaacc agactaaagt gatttatgaa cgataacgtt ttgctcatag
     4201 gagcttccgg attcgtagga acccgactac ttgaaacggc aattgctgac tttaatatca
     4261 agaacctgga caaacagcag agccactttt atccagaaat cacacagatt ggcgatgttc
     4321 gtgatcaaca ggcactcgac caggcgttag ccggttttga cactgttgta ctactggcag
     4381 cggaacaccg cgatgacgtc agccctactt ctctctatta tgatgtcaac gttcagggta
     4441 cccgcaatgt gttggctgcc atggaaaaaa atggcgttaa aaatatcatc tttaccagtt
     4501 ccgttgctgt ttatggtttg aacaaacaca accctgacga aaaccatcca cacgaccctt
     4561 tcaaccacta cggcaaaagt aagtggcagg cagaggaagt gctgcgtgaa tggtataaca
     4621 aatcaccaac agaacgttca ttaaccatca tccgtcctac cgttatcttc ggtgaacgca
     4681 accgcggtaa cgtctataac ttgctgaaac agatcgctgg cggcaagttt atgatggtgg
     4741 gtgcagggac taactataag tccatggctt atgttggaaa cattgttgag tttatcaagt
     4801 acaaactgaa gaatgttgcc gcaggttatg aggtttataa ctacgttgat aagccagacc
     4861 tgaacatgaa ccagttggtt gctgaagttg aacaaagcct gaacaaaaag atcccttcta
     4921 tgcacttgcc ttacccacta ggaatgctgg gtggatattg ttttgatatc cttagcaaaa
     4981 ttacgggcaa aaaatacgct gtcagctcag tgcgcgtgaa aaaattctgc gcaacaacac
     5041 agtttgacgc aacgaaagtg cattcttcag gttttgtggc accgtatacg ctgtcgcaag
     5101 gtctggatcg aactctgcag tatgaattcg ttcatgccaa aaaagacgac ataacgtttg
     5161 tttctgagta atgcagacga gtcagagaaa aaaggaccag gttggactac gattattcgg
     5221 aaagaggtac tgattaaatt atcagtatgt tgatttccga gtaatccgag taacccagat
     5281 gctgatggtg tgtagtgtta tcgtaaggtt taatcaaagt tatgcggaac taagctttgg
     5341 tatatagcca taacgtttgg ctaacctttg gcaataattc acgcactatc agccacttag
     5401 aggtaggaaa agaatgacga atttaaaagc agttataccg gtagcggggc ttgggatgca
     5461 tatgttgcct gccaccaagg cgattcctaa agagatgcta ccgatcgtcg acaagcctat
     5521 gattcagtac attgttgacg agattgtggc tgcagggatc aaagaaatcc tcctggtaac
     5581 gcatgcgtcc aagaacgcgg tcgaaaacca cttcgacact tcttatgaat tagaatcact
     5641 ccttgaacag cgtgtgaagc gccagctgct ggcggaagta cagtccatct gcccgccggg
     5701 cgtgacaatt atgaacgtgc gtcagggtga acctttggga ttaggtcact ccattttgtg
     5761 tgcacgaccc gccattggtg acaatccgtt tgtcgtggtg ctgccagacg ttgtgatcga
     5821 cgacgccagc gctgatccgc tgcgctacaa ccttgctgcc atgattgcgc gcttcaatga
     5881 aacgggccgt agccaggtgc tggcaaaacg tatgccgggt gacctctctg aatactctgt
     5941 catccagacc aaagagccgc tggaccgcga aggtaaagtc agccgcattg ttgaattcat
     6001 cgaaaaaccg gatcagccgc agacgctgga ctcagacatc atggccgttg gtcgctatgt
     6061 gctttctgcc gatatttggc cggaactaga acgtactcag cctggtgcat ggggacgtat
     6121 tcagctgact gatgccattg ccgagctggc gaaaaaacag tccgttgatg caatgctgat
     6181 gaccggcgac agctacgact gcggtaaaaa aatgggctat atgcaggcgt ttgtgaagta
     6241 tgggctgcgc aacctgaaag aaggtattaa gtttagagaa aaaattttaa agttacttta
     6301 attttcatct tatctacggg ggtattgata tcctcgtact tttttataaa cgtttataga
     6361 tagcgctttg gttgctgtga gccaagggcg gtagcgttct atttttaatg aaatattaca
     6421 gaggttaata gagtagagaa atgagcttta a
//
LOCUS       DAZFKF010000190         6362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFKF010000190 DAZFKF010000000
VERSION     DAZFKF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.192"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..66
                     /locus_tag="ACHKA1_005053"
     CDS             <1..66
                     /locus_tag="ACHKA1_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682586.1"
                     /translation="PLKSVMAGDTELLTVGEITWL"
     gene            57..617
                     /locus_tag="ACHKA1_005054"
     CDS             57..617
                     /locus_tag="ACHKA1_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO5682587.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHKEENI"
     gene            617..1528
                     /locus_tag="ACHKA1_005055"
     CDS             617..1528
                     /locus_tag="ACHKA1_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5682588.1"
                     /translation="MFHVDNNSGVANMPELAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENAETARNNLDVYSRSEGDNRYLRR
                     DQNGADIQDKGTFIDNLGLRETVNKAADALPSAGTAVAANRLATPRNINGVPFDGTLD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEMSWNYSAPSGCMLSGINVQETGSR
                     SADNIGGVYYRPVQIYINNTWRTVSSV"
     gene            complement(1563..2084)
                     /locus_tag="ACHKA1_005056"
     CDS             complement(1563..2084)
                     /locus_tag="ACHKA1_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5682589.1"
                     /translation="MQLINVKRYYPKDKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            2441..2626
                     /locus_tag="ACHKA1_005057"
     CDS             2441..2626
                     /locus_tag="ACHKA1_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682590.1"
                     /translation="MFISCVPSKATPLILRAFASLLAATAVPFDGRAFAALFTVSRKP
                     RFQGCRISTLKGFSTSF"
     gene            2640..3200
                     /locus_tag="ACHKA1_005058"
     CDS             2640..3200
                     /locus_tag="ACHKA1_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5682591.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            3300..5339
                     /locus_tag="ACHKA1_005059"
     CDS             3300..5339
                     /locus_tag="ACHKA1_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5682592.1"
                     /translation="MTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMTFAD
                     CLISFNTRDCLWVRPVSGDPSVEITGAGIGAVIPLSADVAGTAEPSDWDNAETHTRPS
                     GNETASSSPSWYYVVVLAGQSNGMAYGEGLPLPETYDRPEPRIMQLARRSTVTPGGKA
                     CQYNDIILADHCLHDVQDMSGKNHPKADVAKGQYGTVGQGLHIAKKLLPFIPADAGIL
                     LVPCCRGGSAFTAGADGTYSDSTGASEDSARWGVDKPLYKDLISRTKAALAKNPKNRL
                     LAVVWMQGEFDIDAKPTEHSALFLAMVEKFRADLAEQAEQCTGGSAAGVPWICGDTTY
                     FWKQKNEPAYQAIYGGYKNKTDKNIHFVPLMTDENGVNVPTNNPAEDPDIESIGYYGS
                     TWRNSAATWTSADRASHFSAWARRGIISDRLATAVLTHAGRTTVKADVPSSETEAPVP
                     SPSETEAVTTTLLSYRVSESEGNLKAQGWEPAGGKAEIISDAGGTGGKAMKLTKETGK
                     SSWYLDHDAGTGAELLKNGGLISCRFKVPGDLVANQYVMALYWPVSSLPQGVTLTGDA
                     GNNLLASFYIQTDAKDLNVMYHNAKVATNNQKLGTFGAFDNEWHTLAFRFAGNNSLQV
                     IPVIDGQDGAAFTLTQSPVGTFPVDKLRVTDITKNATYPVLIDSIVVEVKKAVTE"
     gene            5486..5668
                     /locus_tag="ACHKA1_005060"
     CDS             5486..5668
                     /locus_tag="ACHKA1_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5682593.1"
                     /translation="MTFVHTMLLYFCAVVSALYLVSGGYKVIRNYIRRKIDDAAAEKL
                     SKTAQAPSSPNDPTPL"
     gene            5704..5949
                     /locus_tag="ACHKA1_005061"
     CDS             5704..5949
                     /locus_tag="ACHKA1_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5682594.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENDPQPVEQPEPVQPGTDGTMM"
     gene            complement(5988..6362)
                     /locus_tag="ACHKA1_005062"
                     /pseudo
     CDS             complement(5988..6362)
                     /locus_tag="ACHKA1_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1672 a         1487 c         1624 g         1579 t
ORIGIN      
        1 ccgctgaagt ccgtgatggc cggagataca gagttgctga ccgtggggga aatcacatgg
       61 ctgtaaccct gaccccgcat cagcgcgccc tgttgcagtt gctgcctgac gggctggcat
      121 gggataagcg gccgtcatcc gttcttgcgg ctttgtgcct gggcctcagt cattccacgg
      181 agcgtgtttc ctggaccggt aaccagatgc tggcagaacg ttttcctgat tcatcccgtc
      241 tgctgctgga agactgggag cgttatctgg ggttaccgga atgtgatatg accggcgcaa
      301 ccattcagga acgtcagcgt tatgcaggga ataaataccg gatgaaaccc tctcttaacc
      361 gtgaatttta tatccggttt gcggcagagt ttggttatga aatagatatt cagccatcac
      421 cggattcaca gtgggtcagt attgtcacga taaacagtga aaccggctac cggaatatga
      481 atgtgctgga tgatattctc accccgctgc gtatttatga gggtggtgcg ctggaatgta
      541 ttctgaaccg ttataagcct gcatggcaga cgtttattta cgtgtatgca aacagtcaca
      601 aagaggagaa tatttaatgt tccatgttga taataattcc ggcgtggcga atatgcctga
      661 actggctccg gcgcagagta ataccaccac ctggtttacc gaaggtgacg gacaaaaagg
      721 tatcagctgg attggtcagg actggctgaa tattctccag gccgaactgc tgaatattct
      781 ggctgaagcc agtattcagc cggataaggc gcagttaaac cagcttacgc tgtccattaa
      841 agccattatc gctgcgaatg ccttttcccg gaaaaacaac ctgaaagaaa ttgctgatgc
      901 cggtgcggaa gcccagcgtc tggcccgtgg ttatcttggt ctggggacgc ttgccacaaa
      961 aaacagtctt ggtcccgctg acgttaatgc cctggcgaag gatcagaacc ttgccgacct
     1021 ggagaatgcg gaaaccgccc gtaataatct ggatgtttac agcaggagcg aaggtgataa
     1081 ccgttacctg cgcagggacc agaatggcgc ggatatccag gacaaaggaa cctttatcga
     1141 taacctcggt ttacgggaaa cggtaaataa ggctgctgat gccctgccat cggctggaac
     1201 cgccgtggca gcaaacagac tcgccacccc aagaaatatt aatggtgttc cttttgacgg
     1261 aacgctggat atcaacatca cgtcaggaat gacgcagtca accgcagatg gccggtatgt
     1321 acagaatgtt cagcttggag cacagagcta tcattcaccc ggaggcaatg aaatgtcatg
     1381 gaattacagc gcaccttccg gttgtatgct ttccggtatt aacgtgcagg aaaccggctc
     1441 ccggtctgcg gacaatatcg gtggggtcta ttatcgcccg gttcagattt atattaataa
     1501 tacctggaga acggtaagtt cagtgtaaac cacagaaaag ggtgctgaat gcaccctctg
     1561 aattattccg gtttttccgg ccagggaatc gcctggtaat gcccctcact ggcaatatca
     1621 ttaaagtgca tttctttaag ctgtcggata tacgccatcc agcgcgtcag ctgtaattta
     1681 ttttcatcgc taatcatgtc cagttttaat tctgttttcc agtcatccac ggtttcgttg
     1741 gcttcctgga gcagcgatga gcgacgcttt tcagcaaccg tcttccagtt taccggcacc
     1801 tttgatatca ggccatcaga aaactgccag ttaccgtcaa tattcacacc ttccggcagt
     1861 tcgtccactt caacaacagt aaaacctgcc ggatataaag cagagacatc ttcagcaaca
     1921 gagcagataa tacccgttac aggcgaaata cagagtttgt atttctttgt aaaaagcggg
     1981 atagattcat aaaaatccct tccgtcctca ctctggaaat actgaacatc ctcaccatag
     2041 ggtttgtctt tcgggtaata tcgtttgaca ttaatcagtt gcataaacat caccatgtaa
     2101 atcaatgata aaaacaggaa taatactatc gccatataca ggcaatgctt gtctgaaata
     2161 acgcatattt cagatgctgg ctgctgtgtg ctatgccccc gttaattaaa tacttgcagc
     2221 ggatacccaa tgcccattaa tacaatattg tatctgtctg taataaacac ggtcatcacc
     2281 accacgcatt tcccagtctc cgtcagtctt aaaaccggta atcacatttc ctgatgacag
     2341 ggagtaatta tattccagtc cgccgcttaa tgcctgcgct cccagtctta cccctgtaac
     2401 ataacgctga ttggccgttg attccggcat tcctgacgta atgtttatat cctgcgtgcc
     2461 gtcaaaggcg acaccattga ttctgcgggc atttgccagc ctgcttgctg caacagccgt
     2521 accgtttgat ggcagggcat tcgcggccct gtttaccgta tcccgtaaac cgaggtttca
     2581 gggatgccgt atatctactt taaaaggctt ttccacctct ttttaaggaa ggtaaataca
     2641 tgctgattgg ctatatacgc gtatcaacaa atgaccagaa caccggttta caaagaaatg
     2701 cgctggaatg tgcaggatgt gagcagattt ttgaagacaa aatgagtggt aaaacgacac
     2761 agcggccggg actgaagcgg gcactgaaga aactaaaacc cggtgacacg ctgatggtct
     2821 ggaaactgga caggctgggg cgcagtatgc gccaccttgt tgcgctgaca gatgaattac
     2881 agcaacaggg gataaatttt cgtagtctta ctgacaacat tgatacgtca acccccatgg
     2941 ggcgcttctt ttttcacata atgggtgcgc tggcagaaat ggagcgtgaa ttaattgtcg
     3001 aacggacacg ggctggtctt gcagctgccc gtgaaagggg acgaaccggc ggcagacccc
     3061 gccgtctcac gcaggaacaa tacgagcagg tcgggcgttt actggccggg ggtgtcagtc
     3121 gaagacaggt ggcacttatt tttgatatcg gtctttcaac cctttatcgt tattatcctg
     3181 cgagcgtttc ggaaaaataa gacgctctct ttgttgggcc tttactgaat taacgcaaaa
     3241 tggcgtttat cggtctgtgg atatgataat tcagtttatt caggaacagg ctgattatta
     3301 tgacgtccaa atgggttcaa ctatcatcaa tgcccggaaa ctttactgtt aaagtttccg
     3361 gtggtacagc ggcatttctt gaggctccct ttcctccggc cgaaacaaaa ggaggaatga
     3421 catttgctga ctgtctcatc agttttaata cacgggattg cctgtgggta aggccagtat
     3481 ccggtgatcc gagtgtggaa attaccggag cgggtattgg tgcggtcatt ccgttaagtg
     3541 ctgatgttgc cggtactgct gaaccgtcag actgggataa tgctgaaaca catacccgtc
     3601 cgtcaggaaa tgaaactgct tccagctccc cttcctggta ttacgtggtg gttcttgctg
     3661 gtcagtcaaa cggtatggct tacggtgaag gtctgccact gccggagaca tatgaccgcc
     3721 ccgaaccgcg tattatgcag ttagcccgtc gcagtacagt cacgccggga ggtaaagcct
     3781 gtcagtataa cgacatcatt ctggccgacc actgtctgca tgatgtgcag gatatgagtg
     3841 gaaaaaatca cccgaaagcg gatgtggcta aagggcaata tggcaccgtg ggtcaggggc
     3901 ttcacattgc caaaaaactg ctgccgttta ttccggcaga tgcggggatt ctgctggttc
     3961 cctgctgccg tggtggttct gcattcacgg cgggtgctga cggcacgtac agtgacagca
     4021 cgggtgcttc agaggactca gcccgctggg gtgtggataa gccgctgtat aaggacttaa
     4081 tcagccggac aaaagcggca ctggcgaaga acccgaaaaa ccgcctgctt gctgtggtgt
     4141 ggatgcaggg cgagtttgat attgatgcga agccgacgga gcattctgcg ctgtttctgg
     4201 cgatggtgga aaaattccgc gcagacctgg ctgaacaggc ggaacagtgt accggtggca
     4261 gtgctgctgg cgttccgtgg atttgtggcg ataccacgta tttctggaaa cagaaaaatg
     4321 aaccagcata ccaggcaatc tatggcggct acaaaaacaa aacagataaa aacattcatt
     4381 tcgttccatt aatgacagat gaaaatggcg tgaatgtacc caccaacaat ccggcagaag
     4441 acccggatat tgaatccatt ggttattacg gctcaacgtg gcgtaacagt gccgctacct
     4501 ggacatctgc ggaccgggcc agccatttca gtgcctgggc gcgccggggt attatttctg
     4561 accgtctggc cacggctgtt ctgactcatg cagggcgaac cacagtaaaa gccgatgttc
     4621 catcttctga aactgaagca ccagtgccgt caccttcaga aactgaagca gtaaccacaa
     4681 cactgctgtc ttaccgtgtc agtgagtcag aaggaaatct gaaagcccag ggctgggagc
     4741 cggctggcgg taaagctgaa attatcagtg atgcaggagg cacaggtggt aaggcaatga
     4801 aactgaccaa ggaaacaggt aaatcatcct ggtatctgga tcatgatgcc ggtactggtg
     4861 ctgaactgct gaaaaatggt ggtttaatca gttgtcgttt taaagtacct ggcgacctgg
     4921 tggcaaacca gtatgtcatg gcgctttact ggccggtttc ctctctgccg cagggtgtca
     4981 ccctgacagg ggatgcaggg aataatctgc tggcatcgtt ctacatccag acagacgcaa
     5041 aagacctgaa tgtgatgtac cacaatgcaa aagtggcaac aaataaccag aaactgggaa
     5101 cctttggtgc atttgataat gaatggcata cgctggcctt ccgttttgca ggaaataaca
     5161 gccttcaggt tattccggtt attgatggtc aggatggtgc cgcgtttacc ctgacgcagt
     5221 caccagtagg aactttcccg gtggacaaac tccgcgtaac ggatatcacc aaaaatgcaa
     5281 cttacccggt gctgattgac agcattgtgg tggaagtgaa aaaagcggta acagaataaa
     5341 aaaatcccgc cggtcatgat atggctggcg ggagctacct aacccataaa ggaaaataaa
     5401 aatactcccg aaggagtggt tatcagtcgc ttcgatgttt ttacccgata acgaattatg
     5461 tagtcaatat caaaggtaag gttatatgac attcgttcat acaatgctgc tttatttctg
     5521 cgctgtggtc agtgcgttat atctggtgag cggcggttat aaggtcatca gaaattatat
     5581 tcgccgtaaa attgatgatg cggcagctga aaaactcagt aaaacagcac aggcaccgtc
     5641 atccccaaac gacccgaccc cgctctgata acggggcaat atacctgcaa aaggagaata
     5701 ttcatgccag agattaaagg cacggttact gaagagcttg tcaaacaggc actttattct
     5761 gaagaagtga accgcgtgct gaaggcgcag gttcgcaagg attttgaggc acaaatcgac
     5821 gcatatgttg atgaagtgct ggcccgaatg gttggccgtt ctccggctga aaacagcacg
     5881 gaaaatgatc ctcaacccgt agagcaacca gagccggttc agcccggaac tgacggtact
     5941 atgatgtaac aataccgacc ggcaggtttc cctgccgggt gagtttgtta atgcacgttt
     6001 tgtgtatttt ccctcctggg agaacaggca ggacatatca gcatcctgaa acaggaagca
     6061 tttttgggtt tgtatgtctt gccattttga gatcgagaac ggcgatagat gagctgtaag
     6121 ggagtcttac aggcagggca aacaggctct ttcatccatg ataaagatct cctttttatg
     6181 catctctgct ttacaggaga ctggggaata tcataggata cgccacgagc tcgaagtaaa
     6241 gcattgatgc aaaattgtct aaatcggccc agaatagttc gcgctatctg ggtcgctctt
     6301 tatgtatgta gtatcccatt gatttgaaac atactacata tacgcgcgat cctaccttca
     6361 ct
//
LOCUS       DAZFKF010000191         6291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFKF010000191 DAZFKF010000000
VERSION     DAZFKF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.193"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            86..1015
                     /gene="pfkB"
                     /locus_tag="ACHKA1_005063"
     CDS             86..1015
                     /gene="pfkB"
                     /locus_tag="ACHKA1_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO5682595.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1116..1406
                     /gene="ghoS"
                     /locus_tag="ACHKA1_005064"
     CDS             1116..1406
                     /gene="ghoS"
                     /locus_tag="ACHKA1_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO5682596.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            1512..2372
                     /gene="yniA"
                     /locus_tag="ACHKA1_005065"
     CDS             1512..2372
                     /gene="yniA"
                     /locus_tag="ACHKA1_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO5682597.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2413..2949)
                     /gene="yniB"
                     /locus_tag="ACHKA1_005066"
     CDS             complement(2413..2949)
                     /gene="yniB"
                     /locus_tag="ACHKA1_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO5682598.1"
                     /translation="MTYQQAGRIAVLKRIMGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3096..3764
                     /gene="hxpB"
                     /locus_tag="ACHKA1_005067"
     CDS             3096..3764
                     /gene="hxpB"
                     /locus_tag="ACHKA1_005067"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO5682599.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3927..4517
                     /gene="ydjM"
                     /locus_tag="ACHKA1_005068"
     CDS             3927..4517
                     /gene="ydjM"
                     /locus_tag="ACHKA1_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5682600.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4650..6041
                     /gene="tcyP"
                     /locus_tag="ACHKA1_005069"
     CDS             4650..6041
                     /gene="tcyP"
                     /locus_tag="ACHKA1_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO5682601.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQILKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(6045..>6291)
                     /gene="ydjO"
                     /locus_tag="ACHKA1_005070"
     CDS             complement(6045..>6291)
                     /gene="ydjO"
                     /locus_tag="ACHKA1_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFO5682602.1"
                     /translation="GMGSGILEDIMSIPDSSLRKRLGYEVLSFSLQAHSLSQECIDKL
                     DIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         1546 a         1558 c         1572 g         1615 t
ORIGIN      
        1 ataaagctcc aataaatcat attgttaatt tcttcacttt ccgctgattc ggtgccagac
       61 tgaaatcagc ctataggagg aaatgatggt acgtatctat acgttgacac ttgcgccctc
      121 tctcgatagc gcaacaatta ccccgcaaat ttatcccgaa ggaaaactgc gctgtaccgc
      181 accggtgttc gaacccgggg gcggcggcat caacgtcgcc cgcgccattg cccatcttgg
      241 aggcagtgcc acagcgatct tcccggcggg tggcgcgacc ggcgaacacc tggtttcact
      301 gttggcggat gaaaatgtcc ccgtcgctac tgtagaagcc aaagactgga cccggcagaa
      361 tttacacgta catgtggaag caagcggtga gcagtatcgt tttgttatgc caggcgcggc
      421 attaaatgaa gatgagtttc gccagcttga agagcaagtt ctggaaattg agtccggggc
      481 catcctggtc ataagcggaa gcctgccgcc aggtgtgaag ctggaaaaat taacccaact
      541 gatttccgct gcgcaaaacc aagggatccg ctgcatcatc gacagttctg gcgaagcgtt
      601 aagtgcagca ctggcgattg gtaacatcga gttggttaag cctaaccaaa aagaactcag
      661 tgcgctggtg aatcgcgaac tcacccagcc agacgatgtc cgcaaagccg cgcaagaaat
      721 cgttaatagc ggcaaggcca aacgggttgt cgtttccctg ggtccacaag gagcgctggg
      781 tgttgatagt gaaaactgca ttcaggtggt gccaccaccg gtgaaaagcc agagtaccgt
      841 tggcgctggt gacagcatgg tcggcgcgat gacactgaaa ctggcagaaa atgcctctct
      901 tgaagagatg gttcgttttg gcgtagctgc ggggagtgca gccacactca atcagggaac
      961 acgtctgtgc tcccatgacg atacgcaaaa aatttacgct tacctttccc gctaacaaaa
     1021 acattccccc agcattgggg gaatcatcac caacctgtcg gcaacgcgtt tctccgacta
     1081 tgctcaaaag tcatgtgata acaaaggggt gaactatggc cagtggcgat cttgtccgtt
     1141 atgtcataac cgtcatgttg catgaggata cattgactga aattaacgag ttgaataatt
     1201 acctgactcg cgacggtttt ttgctcacca tgacggatga tgagggaaat atccatgagc
     1261 tggggactaa cacttttgga cttatcagta cccaaagtga agaagaaatt agagaactgg
     1321 tttcggggct tacccaaagt gcaaccggca aagatcctga aatcaccatc acgacctggg
     1381 aggaatggaa tagcaacaga aaataaatgg tttttgggca ataatcagtc tgtggtgtgc
     1441 gttagctcgt gtttttacac cgcattcttg cgctaacctt atgatctggc agacaacatg
     1501 ggagagacat catgtggcag gcaatcagtc gtcttttgag cgagcagtta ggtgaaggcg
     1561 aaatcgaact gcgtaatgaa ctgcctggcg gagaagtcca tgccgcatgg catttgcgct
     1621 atgcaggaca tgactttttc gtcaaatgtg atgaaaggga actgcttccc ggttttaccg
     1681 ccgaagccga ccaactggag ttactgtcgc gtagtaaaac cgtcaccgtg cctaaggttt
     1741 gggcagtagg cgctgaccgt gactacagtt ttctggtgat ggattatctc ccacctcgtc
     1801 cgctggatgc gcatagcgca tttattcttg gtcagcaaat tgcgcgttta catcaatgga
     1861 gtgaccaacc acaatttggc ctcgatttcg ataacgcgct ctccacaact ccacagccca
     1921 acacctggca acgtcgctgg tcaacgtttt ttgctgaaca acggattggc tggcagttgg
     1981 aactggcagc agaaaaaggg atcgctttcg gcaatatcga cgccatcgtc gagcatattc
     2041 agcagcgtct cgcctcacat caaccgcagc cttctctgtt gcacggcgat ttatggtccg
     2101 gcaactgtgc gctgggtccg gatggcccgt acattttcga cccggcatgc tactggggtg
     2161 accgagagtg cgacctggcg atgttaccgc tgcatactga acagccgcca caaatttatg
     2221 acggctatca gtcagtatcc ccgctacctg ccgatttcct tgaacgtcaa ccggtttacc
     2281 aactctacac gctgttaaat cgagcaaggt tatttggcgg tcagcatttg gttattgctc
     2341 agcagtcatt ggatagatta ttagcagcat gatatgggtt gaggataatg gccgctccgt
     2401 gcggcctttt gattaaataa acccaagcag agaaaagaac acataacctg cagcaataca
     2461 aataaccggc aggatataca gtgagaaaaa ctgcaggaaa atagtatggt gcggaacaac
     2521 aatacgggac tcaatttgtt cacgcgttag cccctcttcc cctttggctt tttccagaat
     2581 gagttgatct tcaacacctt cccgcaagaa gcgcgcctgg cgactcatcc gggcaccgga
     2641 atcctgcaac gccagtccga caaaaatcag gatgaaaatc acccagaaca taacgttcac
     2701 gccaccatta aaattgggcg tcggggagtt ataccagaat agattcaaaa aaggcgtatt
     2761 cgcctgcatc atatcgatca tgacatgagt aaagtcgagc atgaccgcat taatgccttc
     2821 ctggttttcc tgtcgggtat tcataaattt cagcaaagaa atcaatgtag agatcaatgc
     2881 aggtataaaa atcacccaac ccataatcct tttcaaaaca gcaatgcgtc cagcttgttg
     2941 atacgtcatg agttctcctt gattaagacg cgtcgtttca cttagtttac ctgtagatat
     3001 ctgttttcgc ccattcttta aaggcgatat gataggcgct taatcataag cacagcttaa
     3061 taccttacac ataatgctct aaaggagagg ttgtaatgtc aaccccgcgt cagattcttg
     3121 ctgcaatttt tgatatggat ggattactta tcgactcaga acctttatgg gatcgagccg
     3181 aactggatgt gatggcaagc ctgggggtgg atatctcccg tcgtaacgag ctgccggaca
     3241 ccttaggttt acgcatcgat atggtggtcg atctttggta cgcccggcaa ccgtggaatg
     3301 ggccaagccg tcaggaagta gtagaacggg ttattgcccg tgccatttca ctggttgaag
     3361 agacacgtcc attattacca ggcgtgcgcg aagccgttgc gttatgcaaa gaacaaggtt
     3421 tattggtggg actggcctcc gcgtcaccac tacatatgct ggaaaaagtg ttgaccatgt
     3481 ttgacttacg cgacagtttc gatgccctcg cctcggccga aaaactgcct tacagcaagc
     3541 cgcatccgca agtatatctc gactgcgcag caaaactggg cgttgaccca ctgacctgcg
     3601 tagcgctaga ggattcggta aatggcatga tcgcctctaa agcagcccgc atgcgttcca
     3661 tcgtcgttcc tgcgccagaa gcgcaaaatg atccacgttt tgtattagca aacgtcaaac
     3721 tctcatcgct gacagaactc accgcaaaag accttctcgg ttaatgacca ggggcagtga
     3781 tcacgctgcc cctggttctt tatctgaatt tgcattcaac tgacggatta atcgtcaatt
     3841 taagagaaag agttacaccg tcaccacttc cgtgcactgt ataaaaatcc tatactgtac
     3901 gtatcgacag tttagtgagt tttatcatga cggcggaagg tcaccttctc ttttctattg
     3961 cttgtgcggt atttgccaaa aatgccgagc tgacgcccgt gctggcacag ggtgactggt
     4021 ggcatattgt cccttccgca atcctgacgt gtttgttacc ggacatcgat cacccaaagt
     4081 cgtttcttgg gcagcgatta aagtggatat caaaaccgat cgcccgcgct tttgggcatc
     4141 gtggttttac ccacagtctg ctggcggtat ttgcgctgct ggcaaccttt taccttaagg
     4201 ttcctgaaag ctggttcatt ccggctgatg cgctacaagg aatggtgctg ggttatttaa
     4261 gccacatact tgccgatatg ctgacacccg ccggtgttcc cctgctctgg ccatgccgct
     4321 ggcgtttccg cttgcctatc ctggttcccc aaaagggtaa ccaactggaa cgttttatct
     4381 gcatggcatt atttgtctgg tcggtatgga tgccccattc attacccgag aacagcgctg
     4441 ttcgttggtc atcgcaaatg atcaatacct tgcagatcca gtttcatcgg cttattaagc
     4501 atcaggttga atactaaaaa ggcaaaaatc acctttctgg aataagcaat tccatttgaa
     4561 tataagagcc agctcacagt tctgttaatc ttgcgccaac actatgactg ctacgcagtg
     4621 atagaaataa taagatcagg agaacgggga tgaactttcc attaattgcg aacatcgtgg
     4681 tgttcgttgt actgctgttt gcgctggctc agacccgcca taaacagtgg agtctggcga
     4741 aaaaagtgct ggtgggtctg gtgatgggtg tggtttttgg ccttgccctg cataccattt
     4801 atggttctga cagccagata cttaaagatt ctgtacagtg gtttaacatc gttggtaacg
     4861 gctatgttca actgctgcaa atgatcgtta tgccgttagt cttcgcctct attctgagcg
     4921 cggttgcccg tctgcataac gcatctcagt taggcaaaat cagttttctg accatcggta
     4981 cgcttttgtt taccacgctg attgcggcgc tggtcggtgt gctggtcacc aacctgtttg
     5041 gtttgacggc tgaaggtctg gttcagggtg gtgcagaaac tgcacgtctg aacgccatcg
     5101 aaagtaacta tgttggtaaa gtctctgacc tgagcgttcc gcagctggtc ttgtccttta
     5161 tcccgaaaaa cccgtttgcc gatctgaccg gagccaatcc gacgtcaatt atcagcgtgg
     5221 taatttttgc cgcattcctc ggtgtagctg cgctgaaact gctgaaggat gatgcgccga
     5281 aaggtgaacg cgtcttaacc gctatcgata ccctgcaaag ctgggtgatg aaactggttc
     5341 gcctggtcat gcagttgacc ccttacggcg ttctggcact aatgaccaaa gtggttgcag
     5401 gttctaacct gcaagacatc atcaaactgg gaagtttcgt tgtcgcgtcc tacctcggtc
     5461 tgctgattat gtttgcagtg catggcattc tgctgggcat taatggcgtg agtccgctga
     5521 agtacttccg taaggtatgg cctgtgctga cgtttgcctt taccagccgt tccagtgctg
     5581 cgtctatccc actgaatgtg gaagcacaaa cgcgtcgtct gggcgttcct gaatccatcg
     5641 ccagtttcgc cgcctctttc ggtgcgacca ttggtcagaa cggctgcgcc ggtttgtatc
     5701 cggcaatgct ggcggtgatg gttgcgccta cggttggcat taacccgctg gacccgatgt
     5761 ggattgcgac gctggtcggt attgttaccg ttagctccgc aggcgttgcc ggtgtcggtg
     5821 gtggtgcaac tttcgccgca ctgattgtgc tgcctgcgat gggcctgcca gtaaccctgg
     5881 tggcgctgtt aatctccgtt gaaccgctta tcgacatggg ccgtacggcg ctaaacgtta
     5941 gtggctcgat gacagctggc acgctgacca gccagtggct gaagcaaacc gataaagcca
     6001 ttctggatag cgaagacgac gccgaactgg cacaccatta attcttatgc tggcaacggt
     6061 ccgttttgta taggggccgt tgccttactt tttaaatgtt ccatcgcggc gatgcatact
     6121 gactcatatt taaacagatc gtcagcgaaa aaaatgtcca acttatcaat acattcctgg
     6181 ctcaatgaat gtgcctgtag cgagaagctt aaaacctcat acccgagacg ttttctaagt
     6241 gaactatctg gaatactcat aatatcttca agtataccac ttcccattcc c
//
LOCUS       DAZFKF010000192         6286 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFKF010000192 DAZFKF010000000
VERSION     DAZFKF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.194"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            200..415
                     /locus_tag="ACHKA1_005071"
     CDS             200..415
                     /locus_tag="ACHKA1_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5682603.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
     gene            828..1019
                     /locus_tag="ACHKA1_005072"
     CDS             828..1019
                     /locus_tag="ACHKA1_005072"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5682604.1"
                     /translation="MCKRLLAIPGFSPLVSQAIKSWMGDGKQFRRGRDASATLGLVPR
                     QFSTGGKQVLLGITKFGEM"
     gene            1161..2180
                     /locus_tag="ACHKA1_005073"
     CDS             1161..2180
                     /locus_tag="ACHKA1_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO5682605.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWVRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKIRLLGISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
     gene            2937..3116
                     /locus_tag="ACHKA1_005074"
                     /pseudo
     CDS             2937..3116
                     /locus_tag="ACHKA1_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            3291..5261
                     /locus_tag="ACHKA1_005075"
     CDS             3291..5261
                     /locus_tag="ACHKA1_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HFO5682606.1"
                     /translation="MYMNVIRTVICTLIILPVGLQTATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVITNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLEPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGVSLWGNVASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVSITDSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDV
                     QFWYQYAPADNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGSRYDR
                     YKSFNPRAGELKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            5280..6071
                     /locus_tag="ACHKA1_005076"
     CDS             5280..6071
                     /locus_tag="ACHKA1_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFO5682607.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAGNGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT         1876 a         1391 c         1518 g         1501 t
ORIGIN      
        1 ggtctgtctt ctttttaata ttaacttatt aattggtgct attcatataa ttctttaaac
       61 agcgaactta tagccaggat tcaatagata tagtaacagg aacatcagta agcgatgaga
      121 tggtcttccc cctccttcac gacaaccttt aattacgtca tgatggataa actttaacca
      181 caaaagtaag agggatacca tgtctcaaca gaaaatcacc ggcatcgatc tggctaaaac
      241 gaacttttat cttttcagta tcaatgctca tggcaaaccc gcaggaagga tcaaactttc
      301 tcgcaatcaa ctgcttaact ggctggtgca acagcccaaa atgactgttg cgatggaggc
      361 tcgcgggcat cccatcactg ggccagggaa atcaggaaac tcgaccatga tgtaatattt
      421 cttccggcgc aacatgttaa agcttatcag cgacgccaga aaaatgatta tgacgacgct
      481 caggcaatag cagaggcctg ccaacatgga actatacgcc ccgtaccaat aaaaactctg
      541 gagtaacagg acgtccagac ttttttgaaa atgcgccggt tggtttcgat ggaaaggaca
      601 caactgataa atcacgttcg tggacttctt gctgaatatg gcattgtgtt cagtaaaggc
      661 gcaacagaat tacgccaaaa attaccggca ttacttgagg atgcggaaaa cgaactcact
      721 gataccatga ggacgttatt gcatcggcag tatatacggt tgattacact agataatgaa
      781 ctggagtggt atgattctga actgaaaaat atgtccgcca agatcctatg tgcaaacgct
      841 tgttagccat tcctggattc agccctctgg ttagtcaggc aataaaaagc tggatggggg
      901 atggaaaaca gtttagacga ggaagagatg catctgcgac tctgggactg gttccccgac
      961 agttcagcac aggtgggaag caggttcttc tgggtataac aaaatttgga gaaatgtaat
     1021 gaacacatcc caccccatta agtcaggtgc tctgtgatgg atgaagggaa atactggaag
     1081 gtgtggagcc tcgcagattt ccggcatcat agtgcccaat acaggatgca taaagctccc
     1141 tcagactgga gactccacaa atgaaatata caccggttgg cgttgatatc gcaaaacatc
     1201 tgattcaggt tcatttcatc gacgagcaca caggtgaagt gattgataag caactacgtc
     1261 gtcaggattt tctgatgttc ttctgtaatc gggaaccctg cctgattggt atggaggcct
     1321 gtagaggctc tcagcactgg gtgcgtgaac tgacaaagct ggggcataaa gtccggttgt
     1381 tacaggccca ctttgttaag gcattcgtca tgggaaataa gaatgatgtg atggatgccc
     1441 gggctatctg gatggctgtt cagcagccag gtaaggacat cgcggttaaa acagaagaac
     1501 aacagtcagt gctggttttg caccgttccc gcagacaatt ggttaagttc cgcaccatgc
     1561 agattaattc tctgcacggc acgctcctag aatttggtaa aaccattcac aaaggtcggg
     1621 cagcgatgga gcgagagctc cctgaagcac tggagcggat gaaagagaga ctgccgccgt
     1681 atctcattac agtgctggaa aaccagtaca accggctgaa tgagctggac tcgctgatag
     1741 aggatattga aaaaaagctt accagcgtgg cgagacagaa tgaaacctgt aagcggttgc
     1801 tggatattcc cggcgttggg ccacttattg caacggcagc ggtggccacc atgggggaag
     1861 catcagcgtt taaatcaggg cgagagttcg cggcttatgt tggtctggtg cccaaacaga
     1921 ctggctcagg agggaaaatc cgcctgctgg ggataagcaa acgtggtaat acatatctgc
     1981 gagccttgtt tattcatagc gcaagggcgg cagcattact gcccaaagaa cctggtccgt
     2041 ggataaccga actgaaaaaa cgtcgtccaa ccagcgtggc aatcgtcgcc atggcaaaca
     2101 aactggcacg aacagtatgg gcgatagcag ctcatggaag taaatacgac aagaatcatg
     2161 tcagtatcag atcatattaa tctctgatac cgataaatat taacttttaa tgaaaggatg
     2221 aatactgaaa ggtaactctg gcggccatag tgatgacaac gacaggtaag accgggactc
     2281 actaaacctg aatattgtgt tgagctaaaa gctcgctgga gaaatgaggt gtgagtcagc
     2341 gaattacata ggggctcgca gcttcatggc tgcaaaaaag ccggatataa agctgcaacc
     2401 tacccgtcac tgtcaaggca atggatgcct tgcaacggga tgtgttcata taatcaatat
     2461 gtcagatcta tgctgataca gggtgccaga gcggtattat tcagagcctc agagaaaact
     2521 gattcacttc gtgtgtgggt taattgtatt cgggaaaagc gtggcttcaa ccgggcagtt
     2581 gttgctcttg caaataaact ccttcggatc gcctgggcaa ttatcgcccg caatgagatg
     2641 tattttcccg cagcaaaaca aaatcaataa tctacgtaaa atcagtagga gtaaagctta
     2701 ccctgaaatt gcaaagaatg cagaatgatg aaaaacaggt cagaccgacg ttacgaaaac
     2761 ctgcaagatg agccaggcca gtaaggccga gtaagcgata aggtcggagc gcgcgaatgt
     2821 ctcatcaagg ccagaagcaa taacttcatg aacaaaccgg atatacgaaa gtaaacctgt
     2881 ccctatcata gttgctgtac tcttgcaata cgggggaaga tcatctcatc acttacgaac
     2941 gattcgtgaa aacgatgaag aaagactaca tcgcgtgcat gccgaaatct aatgtaagaa
     3001 cggcattgca taatcttgca gtggagatcg ggtattacaa tgaaaaccat ccgcatagtg
     3061 cgttaggtta tctctcacca agggaatatc gacgtcagca agtaacgtca ccttagatgc
     3121 agtagttgtc aggaaatagt gactcaaggg caggcttacc atatacactc ttagtggttc
     3181 gactgggtat tagaatagct attaacagat tagcactgaa cactgcgtaa tagtggttgc
     3241 acaaacaaaa tgttatgtta taacattgct gtattgacat atattttcga atgtatatga
     3301 acgtaatcag aactgtcatt tgtacattaa tcatacttcc ggtggggcta cagacagcga
     3361 caagtcattc ttctatggct aaagatacaa tcaccgttgt cgcgacagga aatcagaaca
     3421 cggtatttga aacgccgtcg atggtcagtg tcatcacgaa tgacacgcca tggagtcaga
     3481 atgcggtcac atcagccggt atgctgaaag gtgttgcggg tctcagccag actggtgcag
     3541 gacggaccaa tgggcagacc tttaatctac gcggctatga caaaagcggg gtacttgttc
     3601 ttgttgacgg cgtccgtcaa ctcagtgaca tggcaaaaag cagtggcact tatctcgagc
     3661 cggcactcat caaacgcatc gaagttgtcc gcggaccaaa ctccagtctg tacggcagtg
     3721 gcgggctggg gggggtggtg gacttcagaa ctgccgatgc tgcagatttt cttccccccg
     3781 gagagacaaa cggtgtaagt ctgtggggaa atgtcgccag tggtgaccac agcacaggct
     3841 cggggctcac ctggttcggt aaaaccggaa aaacagatgc gcttctttct gtcattatgc
     3901 gtaaaagagg aaatatctat cagagtgatg gtgagcgtgc acctaacaag gaaaaacctg
     3961 cagccctgtt tgcgaaaggc tctgtcagta taacagacag taacaaagca ggtgccagct
     4021 tgcgtctcta ccagaataac accactgaac cgggcaatcc cactctgaca catggtgaca
     4081 acggcctgcg tgacagaaaa acagcacaaa atgacgtgca gttctggtac cagtacgccc
     4141 ctgcggataa cagcctcatc aatgtaaagt caacgttata tctcagtgat atcactatca
     4201 agacaaacgg tcacaacaaa acagcagaat ggcgaaacaa cagaacctcc ggtgttaatg
     4261 ttgtcaacag gagccatact ctgatttttc cgggagccca tcagttaagt tatggcgctg
     4321 aatattaccg tcagcagcag aagccggaag gctctgccac actatatccg gaaggaaaca
     4381 ttgactttac atcgttgtat ttccaggatg aaatgacaat gaaaagcaac ccggttaaca
     4441 ttatcgtcgg ttcccgttat gaccggtaca agagcttcaa tccccgagcc ggagaactga
     4501 aagccgaacg cctgtccccc agggcggcga tttcagtctc accgacagac tggctgatga
     4561 tgtacggctc catatcctct gcattccgtg cccccacaat ggcagaaatg tacagggatg
     4621 atgtacattt ttaccgcaag ggtaaaccca attactgggt acctaacctt aatctgaaac
     4681 cagaaaataa catcacccgt gagattggcg caggtattca actggacggc ctgcttacag
     4741 acaatgatcg gctgcagttg aaaggcggat atttcggaac ggatgccaga aactatattg
     4801 ccacgcgcgt ggatatgaaa cggatgcgtt cttattctta taatgtatcc cgggcccgta
     4861 tctggggatg ggatatgcag ggtaattacc agtctgatta ttttgactgg atgctttctt
     4921 ataaccgcac ggaaagtatg gatgccagta gcagggaatg gctgggctcc ggcaatcctg
     4981 acacactcat cagtgacatc agtatacctg ttggtcatgg aggagtttct gccggatggc
     5041 gcgctgaact ttcggcagca gccacgcatg tgaaaaaagg cgatccccat caggctggct
     5101 atgccgtaca ttccttttca ctgtcttata agcctgtaag tgttaaaggc tttgaggcat
     5161 cagtgactct ggataatgcc ttcaacaagc ttgccatgaa tggcaaaggt gtgccgcttt
     5221 caggcagaac tgtcagtctt tatacccgtt atcagtggta acctcgcagg aaataaatca
     5281 tgatgaaaaa tacaggctat atcttagctc tttgtctgac agcatcggag catgccctgg
     5341 cccacgatgt ctggattacc ggtaaacagg cagagaacaa cgttaccgca gagattggtt
     5401 atggtcataa tttcccctca aagggcacaa ttcctgacag aaggaatttc tttgaaaatc
     5461 cccggcttta taacgggaaa gagataataa cgctgaagcc aacgtccacg gattatgtct
     5521 ataaaactga gtctgcaggc aacggtaata gctacgttct gtcaacgaaa atgaaaccgg
     5581 gatactggtc gagaacctcg tcaggatgga agcctgtcag tcgggagggc agaaatgatg
     5641 tggcatactg tgagtttgtg actaaatatg caaaatcttt tattcctggt gaacagcaga
     5701 tgccagcaca actctatcag tctccaacag gggatgagct tgaaatcatt ccattatccg
     5761 atatcagtcg tttcggtgaa gatgtgaagc tgaaagttct gtataaaaca tccccgcttg
     5821 ccggagctac tctggagctt gactctgtca gttatctgaa atcatcccgt catacccatg
     5881 cagctgagca taaacattcg gctcataaag cagaacttac ctttgtaagt aatgaggacg
     5941 gtatcatcac tgtaccttcc cttcatgtcg ggcaatggct ggctaaagtc aaaaataaga
     6001 aagtatttcc ggacaagagt ctgtgtgatg aaactgtcga tgtggcaacc ttaagcttct
     6061 cccgaaatta ataatactat tcctgccctg atttatgggc agggtattat caatacgaga
     6121 atagcctacc gacatccgtg taaaaaacca cataatgatt tttcatatcc atttccagat
     6181 atacttatgt aaagatcacc tccaaaaaag cgtaatagta ataaaaatcc ctgaagcacc
     6241 ctgttcaaac cagaaggaat acataacaag acaataaaat gtgaaa
//
LOCUS       DAZFKF010000193         6186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFKF010000193 DAZFKF010000000
VERSION     DAZFKF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.195"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..147
                     /locus_tag="ACHKA1_005077"
     CDS             <1..147
                     /locus_tag="ACHKA1_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO5682608.1"
                     /translation="ECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSG
                     KNVN"
     gene            182..310
                     /locus_tag="ACHKA1_005078"
                     /pseudo
     CDS             182..310
                     /locus_tag="ACHKA1_005078"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(266..454)
                     /locus_tag="ACHKA1_005079"
     CDS             complement(266..454)
                     /locus_tag="ACHKA1_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682609.1"
                     /translation="MSEKPSTGEPRIKLFSGISFRVPAASASLTFRVADNPTEMHSRV
                     ILDKHIAVSGYDSNRLPV"
     gene            558..1379
                     /locus_tag="ACHKA1_005080"
     CDS             558..1379
                     /locus_tag="ACHKA1_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5682610.1"
                     /translation="MRLVSRFGYAANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            1379..1624
                     /locus_tag="ACHKA1_005081"
     CDS             1379..1624
                     /locus_tag="ACHKA1_005081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682611.1"
                     /translation="MKHIPEGVIQAPESPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNGYAYGCPEFQPNIIHP"
     gene            1718..2191
                     /locus_tag="ACHKA1_005082"
     CDS             1718..2191
                     /locus_tag="ACHKA1_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5682612.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFSLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            2207..2683
                     /locus_tag="ACHKA1_005083"
     CDS             2207..2683
                     /locus_tag="ACHKA1_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO5682613.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            2752..2973
                     /locus_tag="ACHKA1_005084"
     CDS             2752..2973
                     /locus_tag="ACHKA1_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO5682614.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3136..3504
                     /locus_tag="ACHKA1_005085"
     CDS             3136..3504
                     /locus_tag="ACHKA1_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO5682615.1"
                     /translation="MSDTHHETNYPDDYNNRPWWGLPCTITPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELILTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            3594..3968
                     /gene="cbtA"
                     /locus_tag="ACHKA1_005086"
     CDS             3594..3968
                     /gene="cbtA"
                     /locus_tag="ACHKA1_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HFO5682616.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQTLLSRLLDQHYGLTLNDTPFS
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAK"
     gene            3965..4456
                     /locus_tag="ACHKA1_005087"
     CDS             3965..4456
                     /locus_tag="ACHKA1_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5682617.1"
                     /translation="MMKLALTLEADSVNVRALNMGRIVVDIDGVNLAELINNVAENGY
                     SLRVVEEFDQQSTCTPPPFTALAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGLPT
                     FDW"
     gene            4476..4673
                     /locus_tag="ACHKA1_005088"
     CDS             4476..4673
                     /locus_tag="ACHKA1_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5682618.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAKEDIRILRQLQFLQQNR"
     gene            4758..5603
                     /locus_tag="ACHKA1_005089"
     CDS             4758..5603
                     /locus_tag="ACHKA1_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5682619.1"
                     /translation="MSAITVSRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAHARDTWYRSLEYDNFPEISKANILSTFEQLHRSKDEVFERGVINVFRGLN
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGLHLITGQQADRLADLERMLHLFSGKPIPDSR
                     ENIIIRLDDHIRSVQGKASYEDEMFNIRYFKKGSAHITFRKPELVDRLNDIIARHYPE
                     MLPSL"
     gene            5838..5999
                     /gene="rhuM"
                     /locus_tag="ACHKA1_005090"
                     /pseudo
     CDS             5838..5999
                     /gene="rhuM"
                     /locus_tag="ACHKA1_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT         1510 a         1672 c         1535 g         1469 t
ORIGIN      
        1 gagtgcgtgg aaaatgtcgc cacaaccgaa atcattaaag caactgaaga aagtaacggc
       61 catcgtgtca gtctgccgtt atcggttttc gatccccagg actaccatcc actgctgatt
      121 accgtttccg gaaaaaatgt taactgatcc ctgaagtcag tttcatgctt cagcctctgc
      181 catacgttgt ggcagaggct atttatcagg agaaaccaga tatgccgggt tacacacaga
      241 gtatgtcctg gctgaaggat ctttctcata cgggcaggcg gttgctgtca taaccgctta
      301 ccgcaatgtg tttatccagg atgacccggg aatgcatttc cgtcgggtta tccgcaacgc
      361 ggaaggtcag cgacgctgac gctgccggaa ctctgaagct gatgccggaa aacagcttaa
      421 tgcgtggctc gccagtggag ggcttctcag acaataaacc tcattaattt cacccatcag
      481 gccgcgtctt ctccgggaga cgcggctttt ttcatttatt acaccagtaa atcttaacca
      541 ccgataagga gcaaagtatg cgattagtca gtcgttttgg ttatgctgca aaccagatac
      601 gccgtgaccg tccgctgaca catgaagagc tgatgcacca tgtgccgggg attttcgggg
      661 aagagaaaca cacgtcccga agccagaact atacgtacat ccccaccatc accgtactgg
      721 aaagtctgca gcgggaaggc ttccagccct ttttcgcctg ccagacccgt gtgcgcgacc
      781 cgggccgccg gggatacaca aaacacatgc tgcgtctgcg gcgggccgga gagataaacg
      841 gagaacatgt ccctgaaatt attctgctca actcccatga cggtacctcc agctaccaga
      901 tgctgccggg ttacttcagg ttcgtctgcc agaacggatg cgtctgtggt cagtctctgg
      961 gggaagtgcg tgttccgcac cggggaaatg tggtggacag agtcattgaa ggggcttacg
     1021 aggtggtggg cgtgtttgac cggatagagg agaagcgtga tgccatgcag tcgctggtcc
     1081 tgccgccacc ggcacgccag gcgctggcac aggcggcact gacttaccgt tatggtgacg
     1141 aacatcagcc cgtcactacc gccgacattc tgacaccgcg acgccgggag gattacggta
     1201 aggacctgtg gagtgcttat cagaccatcc aggagaatat gctgaaaggc gggatttccg
     1261 gtcgcagtgc aaaaggaaaa cgtatccaca cccgtgccat tcacagcatc gacaccgata
     1321 ttaagctcaa ccgcgcattg tgggtgatgg cagaaacgct gctggagagc atgcgctgat
     1381 gaaacatatc cctgaaggcg tgattcaggc accagaatca cccgacgccc cggaagcgct
     1441 gccggggtgt cgttccattt ctgatgatga cctgtgcgcc tggtgtaccc gtctgcttta
     1501 ccgtcccgga gaactcagtc tgtgcagact gagtacaggc aatggcatct ggccatccgt
     1561 ctgtgaccgg aacggttacg cgtacggctg tccggaattt cagccgaata tcattcatcc
     1621 ctgatattgc cgggtctcac ggtcagtgat gaacgtgatg ttgtcagccc ccggccttta
     1681 tttcctctca tttattcaca ttacaaggat ttcacacatg aaaacattgt ctcagaacac
     1741 cacctcttca gcctgtgcac cggagactgg cctgcaacaa ctggttgcca ccatagtccc
     1801 tgatgaacag cgcatcagct tctggccgca gcattttagc ctcattccac agtgggtgac
     1861 cctggagccc cgtgtcttcg gctggatgga ccgtctgtgc gaagactact gcgggggcat
     1921 ctggaatctg tacaccctga acaacggcgg ggcatttatg gcccctgaac cggatgacga
     1981 tgatgacgaa acatgggtgc tgtttaacgc catgaacggc aaccgcgctg aaatgagccc
     2041 ggaagccgcc ggcattgctg cctgtctgat gacgtacagt catcatgcct gtcgcacgga
     2101 gtgttatgcc atgacggttc attattaccg tctgcgggac tacgccctgc agcatccgga
     2161 atgcagcgcc attatgcgca tcattgactg aaagggggcc ggaacaatgc aacaactttc
     2221 ctttctgccc ggagagatga cgcccggcga gcgcagtctc attctgcggg ccctgaaaac
     2281 cctggaccgc catcttcatg aacccggcgt ggccttcacc tccacccgtg cggcacggga
     2341 atggctgatt ctgaacatgg cgggactgga gcgtgaagaa ttccgggtgc tgtatctgaa
     2401 taaccagaat cagctgattg ccggtgaaac cctcttcacc ggcaccatca accgcacaga
     2461 agtccatccc cgggaagtga ttaaacgcgc cctgtatcac aatgccgcag ccgtggtgct
     2521 ggcacacaat cacccgtccg gtgaagtcac tcccagtaag gcagaccgcc ttatcacgga
     2581 acgcctggta caggcactgg gcctggtgga tatccgggtg ccggaccatc tgatagtcgg
     2641 tggcagccag gttttctcct ttgcggaaca cggtctgctt taacccgtca ccgccacaat
     2701 cacatcacac ctgttttcac ttttatcttc cgtcttcaga ggtatcctat tatgaaaatt
     2761 atcacccgtg gtgaagccat gcgtattcac caacaacatc cgacatcccg tctttttccg
     2821 ttctgtaccg gtaaataccg ctggcacggc agtgctgaag cgtataccgg tcgtgaagtg
     2881 caggatattc ccggtgtgct ggccgtgttt gctgaacgcc gtaaggacag ttttggcccg
     2941 tatgtccggc tgatgagcgt cactctgaac taatgttccg tcacccggaa aacgagtacg
     3001 accagatgac ctcagacgcc gctgtggatg aggcccttct catcaatgaa tacatcttca
     3061 ccgaagccgg tctccacacc ggctgacctg tcatcgttaa ccccttctta accgattttc
     3121 tgtgaggact ttatcgtgtc agacacgcac catgagacaa attatcccga cgactataac
     3181 aaccgcccct ggtgggggct gccctgcacc ataacaccct gttttggcgc ccgtctggtg
     3241 caggagggta accggttgta ttaccttgcc gaccgcgccg gtatcagagg ccggttcagc
     3301 gacgcggatg cgtaccatct ggaccaagcc tttccgctgc tgatgaaaca actggaactc
     3361 atactcacca gcggtgaact gaatccccgc catcagcata ccgtcacgct gtatgccaga
     3421 ggactgacct gcgaagccga caccctcggc tcctgtggtt acgtttatat ggctgtttat
     3481 ccgacgcccg aaacgaaaaa gtaaatcatc agaataacct tctgctccgg cctggtgttt
     3541 tcaccacgcc actttcccat ttttcatctc tgcatatcag gaaaatcttc agtatgaaaa
     3601 cattacctgt attacccggg caggcggcca gttcgcgtcc gtctcctgtt gaaatctggc
     3661 agacactgct gtcccgcctg ctggaccagc attacggcct tacgctgaat gacacaccgt
     3721 tctccgatga acgtgtgatt gagcagcata ttgaggcagg catttcactg tgtgacgcgg
     3781 tgaactttct cgttgaaaaa tacgcgctgg tgcgtaccga ccagccccgg ttcagcactt
     3841 gtccccgctc tcagttaata aacagtattg atatcctccg ggctcgccgg gcaaccggcc
     3901 tgatgacccg cgacaattac agaacggtaa ataacattac ccggggtaag catccggagg
     3961 cgaaatgatg aaactggccc tgacgctgga agctgacagc gttaacgtgc gggcgctgaa
     4021 catggggcgc attgtcgttg acattgatgg tgttaatctc gctgaactga ttaacaatgt
     4081 cgctgaaaac ggttattcac tccgcgtggt ggaggaattc gaccaacagt caacctgcac
     4141 accaccaccg tttacggccc ttgccggcat acgctgcagt accgcacata tcacggaaaa
     4201 ggacaacgcc tggctgtact cgctgtcgca tcagaccagt gacgttggtg aatcagaatg
     4261 gattcatttc acaggtagcg gatatctgtt acgtaccgat gcgtggtcat acccggttct
     4321 gcggcttaaa cgcctgggcc tgtcaagaac gttccgtcgt ctggtcgtca ctctcacccg
     4381 gcgttatggc gtcagtctaa ttcatctgga cgccggcgca gaatgtctgc cgggtttacc
     4441 cactttcgac tggtaatcag gacaaaagac actggatgaa attattaacc acagaaacgg
     4501 cgctggatat tctgattgcg tggctgcagg acaatattga ctgcgaatca gggattatct
     4561 tcgacaacga tgaggataaa acggattcgg cagcactgtt gccctgtatt gaacaggcca
     4621 aggaagatat ccgtatcctg cgccaactgc agtttctgca acaaaaccgg tgagtctcac
     4681 tcaccatact tcattccact gacgccagcc tgaacagggc tggcgttttc atttatctgc
     4741 gaaaaggaat atcgattatg tctgcaatca cagtctcccg tccggaagtg gtcaccgggc
     4801 atacggacgt tatttgctca accagtatcc gccacattct ggctgtacga aagagtacac
     4861 tgctgcaaat cgacacactt atccggcaac tggctgaaat ctcagcaatg acagaaagta
     4921 ttggcggtaa aaccgcactg gactgggcga tgaaacagga ttttcgctgt ggctgctggc
     4981 tcatggaaaa acctgaaacg gcaatgaaag ccatcacacg caatctcgat cgcgaaatct
     5041 ggcgtgacct gatgcaacgt tccgggatgc tgagtctgat ggatgcgcat gcccgggaca
     5101 catggtaccg gtcactggaa tacgataact ttccggaaat cagcaaggcg aatatcctga
     5161 gtacattcga acaactgcac cggagtaagg atgaggtgtt tgagcgcggg gtgatcaacg
     5221 tcttcagagg gctgaactgg aattacaaaa ccaattcccc ctgtaaattt ggcagtaaaa
     5281 ttatcgtcaa caatctggtg aggtgggacc gatggggatt gcatcttatc accgggcaac
     5341 aggcagatcg actcgccgac ctggaaagaa tgctgcatct gttcagcggc aaaccgatcc
     5401 ccgacagccg ggaaaatatc atcattcggc tggatgatca catccgctcc gttcagggca
     5461 aagcgagcta tgaagatgag atgttcaaca tcagatactt taagaagggc tccgcacaca
     5521 tcacgttcag gaaaccagaa ctggttgatc ggctaaatga tattattgcc aggcactatc
     5581 cggaaatgtt accttcatta taaagctcta agaatgaatg caattatgta ttttagcttc
     5641 attccctcac gcaaatttat ttgggggttc ttatgggggt tattcattta tccaacacag
     5701 attaatcatt taaaatcaat atattaatac tcatattcga ctcctgtgat cttccgccaa
     5761 aatgcctctt catacgttct ccaacccctc gtaatactgt caatccagat attttatact
     5821 gctcaatatt caatcggctt caggaatacc tggtcaaagg ttttgtgatg gacgatgagc
     5881 agctgaagaa tccgcctgtc ggttcttctg tcctcgaacg catccgcgat attcgcgcca
     5941 gcatgcgccg tgtttattta caggtgattt tcccccctgc tgactctcag ccaactgtat
     6001 cgccattaag gtgaagatgt tgttttacac gctccagaac accctttttt tatacgactg
     6061 ttctattcct ttaacagctg gcaaaaaaac tgacctataa acttcaccaa taatcagact
     6121 gccatcaaga ttaatttgtt catttaatat atcaaatcaa cttgttttat ttttactatc
     6181 aaaaat
//
LOCUS       DAZFKF010000194         6114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFKF010000194 DAZFKF010000000
VERSION     DAZFKF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.196"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..303
                     /locus_tag="ACHKA1_005091"
     CDS             <1..303
                     /locus_tag="ACHKA1_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO5682620.1"
                     /translation="SKKMWRSNYAPPLLRILWRLGIRLPPLPFMPFWQVTLLMGSLWG
                     ISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLTASFHWWRRKVNRLPPWDDV"
     gene            complement(321..911)
                     /locus_tag="ACHKA1_005092"
     CDS             complement(321..911)
                     /locus_tag="ACHKA1_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682621.1"
                     /translation="MTQSRQPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLIPATGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQVNESITP"
     gene            complement(911..1168)
                     /locus_tag="ACHKA1_005093"
     CDS             complement(911..1168)
                     /locus_tag="ACHKA1_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682622.1"
                     /translation="MDEERVFSLSYEQLTRLAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            1765..2178
                     /locus_tag="ACHKA1_005094"
     CDS             1765..2178
                     /locus_tag="ACHKA1_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682623.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLDGRDALELIFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            2180..2959
                     /locus_tag="ACHKA1_005095"
     CDS             2180..2959
                     /locus_tag="ACHKA1_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO5682624.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            3139..3783
                     /locus_tag="ACHKA1_005096"
     CDS             3139..3783
                     /locus_tag="ACHKA1_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO5682625.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            3870..4178
                     /locus_tag="ACHKA1_005097"
     CDS             3870..4178
                     /locus_tag="ACHKA1_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFO5682626.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            4591..5571
                     /locus_tag="ACHKA1_005098"
     CDS             4591..5571
                     /locus_tag="ACHKA1_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO5682627.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGADIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            5564..5980
                     /locus_tag="ACHKA1_005099"
     CDS             5564..5980
                     /locus_tag="ACHKA1_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO5682628.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(5982..>6114)
                     /locus_tag="ACHKA1_005100"
     CDS             complement(5982..>6114)
                     /locus_tag="ACHKA1_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HFO5682629.1"
                     /translation="NQSGKGKVWFAGRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
BASE COUNT         1480 a         1426 c         1559 g         1649 t
ORIGIN      
        1 agcaaaaaga tgtggcggag caattatgcc cctcctcttc tgcgtattct ctggcggctg
       61 ggtatccggc ttccgccatt accctttatg ccgttctggc aggtcacgct gttgatgggg
      121 agcttatggg gcatttcctg gggatgtgca atgtggttta tctactgggg gccgtcagga
      181 atggtggcag gtgaggccat tattataagt atcacgggtg gttttttgtt cggactgctc
      241 acggcctctt tccactggtg gcgcaggaaa gttaaccggc tgccaccatg ggatgatgtg
      301 taaaccgtgc aggcatgttt ttacggggtg atgctttcat taacctgcac acggacctcc
      361 attcctgcat cgtgacaggc ctgcagccac tgcgccactt ccagcggatc gccctcccgg
      421 cgtaccactc tgccttcttt attccataac tgcagacagg tgctgccgtc gagacgcacc
      481 acaaaatccc cacggcaggc ctgatacgtt atgccgttca gctccaccgc cagatcagtg
      541 gcagaagtgc cgtctgcggg cagacctgtt gccgggatca gcggcagtac gacgacttct
      601 gccgcacgct gttctgcccg gaggcggtcc tgttctgcca gcagcagagg ctgtgccacg
      661 gcccggccgg cgtccgtcag ctccactgcc agctgcaggt tcggagcgcg cagggtacgc
      721 agccagccgg catcctccag acgacggcag gaggcgcgca ggtttggccc gtagaccggg
      781 gcttccccgc ttcgctccag cacccgttct atgtcccgcg tcagcacggg gcctggccgt
      841 ttttcatcaa gggcagccag cacaatcagc actctccgct gcagtggcga cggttggcgg
      901 ctctgggtca ttacctgtca tcctccggcc agatgaggtt tctgaggcgc tgaaagtcgg
      961 cgtcaatgag ctcctgtctc tttatggcat tcattgaggc atagccattt atggccagtt
     1021 catgccagaa gatcagcacg gctccggctt tggctgactc gcagagataa atcgcgccct
     1081 ggctgtccag attgcattcc cggatgcgtt tttccgcaag ccgggtcagt tgttcatagc
     1141 tgagtgaaaa caccctttct tcatccatcc tgcgcgctcc atctgataat gttctgacag
     1201 gtaaaaatca aattctatat gatttttgtg ttattgattt ctgataatat cagatattgt
     1261 ttcattacgg atgattaaaa tcatgtctga tatgaattta tgtgtgttgc ttaattatca
     1321 tatcgcctgc ccgtttcccg ccgcgcagat ccggtttccg gcttttctgc cgcgcagatc
     1381 cgaaaaccct gagtcacgga tcttcctctc cctcacacaa cgccatctcc tgtcagtaca
     1441 acatgtcgtg ctgcattcag ctgctcatga cactatatat tgtgttattt ccctgacctg
     1501 tgatgcgtcg cgcaggggcg gaaaacagcg atatgattat ttccccagcg tggtacactt
     1561 ccggaaagtt gtgatattca ggaaagtcgg atctgacgga aacggctctc cggtaattta
     1621 acggcgtggt tatatggatg cttgttatca tggtgatgat gataacggca tgatgttatc
     1681 agacggcgtg acggtaaggg cagtgatgat ggatgacgtt atcgcatgac cgtccctgcc
     1741 cggaaaagaa aaaaggagtc acccatgttt tttattgaga atgaaggtca ggctgtcgcc
     1801 agaacggatt actggcagtc tgtacaggcg caggccggat atgtctacct cagctggaat
     1861 gccggcgcag ccaggctgct tgtcccggat gcggcaaaac atttactcag ggagatgcgg
     1921 ggggctgagt acgtcatcat cagtaaggga acgctggatg gccgcgatgc gctggaactg
     1981 atatttgaag acggcagcga tgcgccgttt gtgatccaca tgctgagtga gcagtgcgat
     2041 cgcctgctcc ccgaaaacaa ccagggaggg ggttttgttg tcaccgtctg gacgcgtggc
     2101 ggtaaccagc tccgttatcc gggaaaatac cgggttgtgg aaaacctgcc cgacgtttcc
     2161 ccgtggagtg agcactgata tgcagcacct gccggcaccg atccaccatg cccgggatgc
     2221 cgctcagctt cctgttgcca tcgattatcc ggcagcgctg gcactccgcc agatgtcgat
     2281 ggttcatgat gaactgccaa aatacctgct ggcccctgaa gtgagcgccc tgctccatta
     2341 cgtgccggat ctgcgccgca agatgctgct ggccacactg tggaacaccg gtgcgcgcat
     2401 taatgaagca ctggcgctga cgcgggggga tttttcgctt gcgcctccgt atccgtttgt
     2461 gcagcttgcg accctgaaac aacggacgga aaaagccgcc aggacggcag gaagaatgcc
     2521 cgccggtcag cagactcacc ggctggttcc gctctccgac gcctggtacg tcagccagct
     2581 gcagacgatg gtggccacac tgaaaatccc catggaacgg cgtaataaac gaacaggcag
     2641 gacagagaaa gcgcggatct gggaagtgac ggacagaacg gtcaggacct ggattgggga
     2701 ggcggttgcc gccgctgctg ctgacggtgt gacgttctct gtcccggtca cgccacatac
     2761 gttccgccat tcctatgcga tgcacatgct gtatgccggt ataccgctga aggtcctgca
     2821 gagcctgatg gggcataagt ccatcagctc aacagaggtc tacacgaagg tgtttgcact
     2881 ggatgtggct gcacggcacc gggtgcagtt ttcgatgcct gagtccgatg cggtgtctat
     2941 gctcaaaaga attccttaag ctctgacttt tttaaatccc tttcggatat ctcatggata
     3001 tattatcggt atttcatatt ctgttgacat gcaggaccgg tatagagtaa aaaaacatct
     3061 attgagtacc tcttggatat ccgaaaggtg tcttaggggg tctttatggg tattacatgt
     3121 ggattctgga gatacactat gcctgttatt gctaatacgc atcccaaagg tggtgtgggt
     3181 aagacaactt catccgtaaa cattgttggt gaaatgaagt ctgatacagt tgatctggat
     3241 actcataccg ggctttctat cattctgggg ctgagacctg aaggaaaaga aatttccgtg
     3301 aaagtaccca aaacagtgga tgagttaatc gaaattatga ctccctacaa gaacagcgat
     3361 aagacgttac ttattgactg tgggggattt gactcagatc ttacccgtac tgcgattgcc
     3421 tttgcagact gcgttattgt cccttcaaaa gactccctga ctgaacgtat tggtctgatg
     3481 cattttgatg gagtactgga cgaaatcagc tctatcatgg ggaccgatat aactgctcac
     3541 ctgtatctct gtaaagttaa tcctaacaag aagaaattcc ctaagcttga tgcaatcctg
     3601 ccatcgttca aacatctgaa gctgatgaag agccgcattt ctgctcgtgc agaatttgag
     3661 gatgtcattg agactgggat gggaatcact gagtctgttc atgggcgcta ttcggcagga
     3721 ggtaaggaag ttattgccct gatcgaagaa atcaatcatc tgattgaaaa taacaaacag
     3781 taaggtgtat ctattgggta ttcatatagt atcctttggg cttttaatgg atatctttta
     3841 agtatctaac aaacatcctg ggttatctca tgagcaaagc gaaatttagt gcacaggctg
     3901 aaaaactgaa aaacctggtg gattctgcca gattacaaac ttcttcttct gccgtaacag
     3961 caacagcccc gtctcagcca aatgaaggtg cagaggaagt ttcaggtcgt cccgtggctg
     4021 ccaggacgaa ggctaagaat cttaaggcca taccgctgtc gtattttgaa gctcatgcgg
     4081 aacttaaagc gaccagtaaa acatctctgg attttagctc atatattatt gaggctataa
     4141 gagagaagct tgaacgtgat ggtgcaatag gtcaataaac tatcggatac cttaagggta
     4201 tctgatgtgt gtcttttaaa tgcgttatgg ggattcttca cgtacctaaa gagtccccat
     4261 tagacatctt tctgtttatg gtgacatgat tcgtcaccgc tttcctttcc tctcagtttc
     4321 accatttcat tcctttccct taacctgaac catgatgtcc acgatgtttt tatattgcat
     4381 ttatgtatca catataacct tcataatgtt tttgtatcta tgatttcctc tggtcttaaa
     4441 gttgtgcgta caatgtacat gcaatttatc gtgtttttat gttgcatttg tcctttcaga
     4501 aagaccgttt tttcattata attgaatata cattgcatgt atgaagcatg tttgtttttt
     4561 ctatttgttt atgagcaacg gagactggtt atgaaaatct ttattgatga tggttctacc
     4621 aacattaaac ttgcctggtt ggaggatggt gacgtgaaaa cgttaatcag ccctaacagc
     4681 tttaaaccgg aatggtcttt tagtttactg gatgatgctg ctccggctaa ttacgagatc
     4741 gacggcgaga agttttcatt tgatccatta agtgctgatg ctgtcgtaac gactgaaaca
     4801 cgctatcagt acagtgatgt gaatgttgtg gccattcagc atgcgctaca gcagaccggc
     4861 ctgaaagcgc agcctgttga tgtcatcgtc acattgccga tctctgaata ccttgacgct
     4921 aacaatcaga aaaataagca aaacattgaa agaaagaaaa aaaatgtgat gcgtgaagta
     4981 cgggtgcagg ggagtgatgc atttgttatt cgcagtgttt ccgttcttcc tgaaagtatt
     5041 cccgctggtt tcagtgttct tgcggggctt gaggatgatg aatccctttt gatcgtagat
     5101 ctcggtggta caactcttga tgtgtcgcat gttcgtagca aaatgacagg gattacaaaa
     5161 acctggtgtg atccgaatat cggcgtgtcc ctgattacat ccggggttaa ggaacagatg
     5221 gctgtacatg ctaatacaag agtgagctca ttccaggctg acaatatcat tgtgcaccgt
     5281 aatgaacctg actacctttc ccgtcgcatt tataatgctg aacagcgtga gtctatcatt
     5341 aatgttatca atgaacggca gaagttgctt attaaacgag ttaatgatgt catcagccgt
     5401 ttcaccgact atacacatgt aatgtgtgtt ggtggtgggg ctgacattgt ggccgaggct
     5461 gtgaagaacc tgactaaggt tcctgatgag cgcttttatc tgagctcatc cccgcagttt
     5521 gatttagtca tgggaatgat aaaaatgaaa ggtggtgtga ctaatgagtg atgaaaataa
     5581 atccagacgc tgctcatttg aacttttccc ggatgaacgg acaggagata agatagcaga
     5641 tgagctcatt gcaaatgaaa aactgaagga gcggggacgt ttcatgcgcg cgatgttagt
     5701 tactggtgcg gcgtttgcag ctatagataa acgtcttcct ttgctgatat cggagttact
     5761 gacagaaaat acgactctgg atgatattaa taaggtgatt tccagcgtaa taccaggtgc
     5821 tttttcggtt gaaaagaaat tgcttgaatt actggaaaaa caatctggtc tccatacgtc
     5881 tgtggattgc agtactcctc ttacagagca gagtctgtcg agaaatgacg gtgaagacca
     5941 gaccagaaga aacgctgaaa atatgttcgg agatgactga actaacccag cctggcaagg
     6001 ggaatatctc gccatttagt tgtataacac tgactcaaca tttcccgttt catttgccat
     6061 tcaggggcgg ttcctcgccc cgcaaaccac actttccctt ttcccgactg gttc
//
LOCUS       DAZFKF010000195         6100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFKF010000195 DAZFKF010000000
VERSION     DAZFKF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.197"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..2151)
                     /locus_tag="ACHKA1_005101"
     CDS             complement(<1..2151)
                     /locus_tag="ACHKA1_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO5682630.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASVVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSVAATSAGAAKTSETNAAASEQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASLSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSAKAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTDCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSTGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVTALTDNTQGA"
     gene            complement(2216..2815)
                     /locus_tag="ACHKA1_005102"
     CDS             complement(2216..2815)
                     /locus_tag="ACHKA1_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5682631.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDILGLVTSFSYAGDKNRQLSRYSDTRWHEDSVRNRWFSVMVG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIMGVGYKF"
     gene            complement(2882..>6100)
                     /locus_tag="ACHKA1_005103"
     CDS             complement(2882..>6100)
                     /locus_tag="ACHKA1_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5682632.1"
                     /translation="IAGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITRTI
                     TSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTTSQ
                     YLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALVGV
                     QVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCLWD
                     MLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRKAW
                     DVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSALKD
                     RHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLWLI
                     KTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLDRE
                     ITLPSSGTTLISLVDGEGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLRQ
                     RLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTA
                     EVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFRQLA
                     LGNYSLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTV
                     QFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKSAF
                     VEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDANDK
                     WNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMF
                     VAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGTLN
                     NVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQVMI
                     PPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMPAG
                     QGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIN"
BASE COUNT         1163 a         1829 c         1583 g         1525 t
ORIGIN      
        1 tgccccctgc gtattgtctg tcagcgcggt tacccgacct tttgaagcca gattaactga
       61 tgatactgct gttccacctg acggtaacgc cccgatctct gatgccgttg gcttatttct
      121 ggagttatag tcccttcgcc agccaggtga ataatctgtt ccgtgattaa tataggtaaa
      181 ctgggcgtta gttgttccac caccagtgga ggtggtcggt gtggtaatgc ggatcgtcat
      241 cgctgacttt atccccatta cttcaatgac agctccggcg agatgaatat taccgcagtc
      301 agtatcagta atgattttat tattgccata agaccaggaa cccttgcaca tccagtaggg
      361 atggttaaat gctccctgag aatccagcca ctcgataaac tgtgcagtcg tccagtttcc
      421 tgttgttgtg cttactgacc caccgaaggc acggcaggca ccaatatttt tcgtaaaggt
      481 gtctttgcca gggatatccg caccgttctg atctttctgc agacgtttct cagcattgtc
      541 attggctgct tttactgcct ttggcgttgc cgccagcgtt tcagacgtgc tgttggtcgc
      601 gctgcttagc tggattatcc ctttctgtgc tgtcgttgca tcctgtgcgg tgtatttccc
      661 gttagccagg tcatacgcgg ccttaacggc ttttggtgtt gccgccagtg actcggaagt
      721 gctgttagtc gcactactga gctgtactat cccctttttc gtcgtgctcg catcctcaag
      781 cgccacggcg gatgcaatat cctctgcccg ttttgccgct gtctcagcgc gcgttgctgc
      841 agattccgcc gtacttttgc tctgagctgc tgccttcgca ctaccagcag cctctgtcgc
      901 cttcgtggat gccgtcgtgg cgctgccctt cgctgctgac gcctgtctgg tcgcctcatc
      961 ttttgaagca gacgccgatg atgccgatga cgccgccgaa ctggcggacg atgccgctgc
     1021 cgtttttgag gattctgcac gggtttccga cgctttcgcg ttcgtttcgg atgtcttcgc
     1081 tgcggaagct gacctcgctg ctgccgtggc ctgctcagtg gcttcgccag ccttcgttgt
     1141 ggctgttgaa gcggatgatg cggcgctttc tgccgatttt ccggcggcgg tggcactggc
     1201 tgaggcctgc ccggcacttg ttgacgcggc actggcagac gacgcagccg ctgtttttga
     1261 gcctgccgca gctgaggcgc tctgtcccgc tgccgtctca gaagacctgg cgttcgtctc
     1321 ggacgttttt gccgccttcg cggaatttcc tgccgccgtt gccgaggaag ctgcgctact
     1381 ggcgctcaag gatgcgttcg tttctgatga tttcgctgcc tcttttgagg ccgccgcatc
     1441 ccgggctgag gtggcagctt ctgacgcttt cgtggtcgcg gtggatgcag aagtggcggc
     1501 tgattgttct gacgctgcgg cattcgtttc tgacgttttc gccgcaccgg cactggtagc
     1561 tgccacgctt tttgaggact ctgcagcggc agcacttttt gaggcttcag tggcctttgt
     1621 tgatgccgtt cctgcgctgg aagacgctga ctgagccgac gaagcggcct gtccggctga
     1681 cgtgctggct gcacgtgctg aatctgcagc atcagtcgca tgggttgccg cctcacgggc
     1741 agatgtgctg gcatcactgg ctgacttctt cgcggctgcc gtgttctgtg ccaccacgga
     1801 cgcgttacgc gccacctctt ccaccatcag ttcaaaacgg cgcagtgcct caggacgggc
     1861 atcatcctcc gtcatggcac cgagaaaatc attcagcgta ccgggtcggg aatcttcata
     1921 cacggtgatg gtcccggcat gtgacggcgg gaatccttcc accaacagaa taacgctgta
     1981 ctgaccgtac tcaacgtcca tgctgtaacg cccggcttca tccggatttt ctgaggccag
     2041 cgtgttcacc accaccgtgg tgctgttacg ttttgctttc agctggattg tgcagttctg
     2101 taccggtttt cctgtgccgt ctttcagtac acctgaaatc tttactgcca tattcacccc
     2161 acaaaaaagc ccgcctgaac cggcgggctg tcataacact gtgttacctg gctaatcaga
     2221 acttataacc gacacccatg atgaaaccgt cagtgcgcca gtcgccactg ccggagcctt
     2281 cataagcaat atcaatggcc acggattcgg tcgggttaaa ctgcacgcca gctccccacg
     2341 ccagagacgt gttgctgtgg cgaccgtcat cacttccggt cagcacgtcg tgcgttttcc
     2401 ccttgttgtc agttacgcgg agataatccc cggagaaagt cgacacacgg ctgtaagcca
     2461 cacccgccat cgcatacgcg ctgaaccatt cattcacgcg cacagacggc cccaccatca
     2521 cgctgaacca gcggttacga acggaatctt catgccagcg ggtatcgctg taacggctca
     2581 gctggcgatt cttgtctcct gcatagctga atgacgtcac cagccccagt atgtccgtaa
     2641 actcataacg gtatttcacg ttaatcccgt tcagatcatc gctgccggga acgttcgtcg
     2701 aggcatgaag ataccccgcg ctcagcgtgg actgatgttc agacgcccat gcaggcgcac
     2761 cggatacggc cagacaaatg gctgcggaca aaatggcggc ataaagttta cgcataatta
     2821 cctctcgctt ttctgcaata aaaaaggcgt catttctgac gcccgttatg ggttataaaa
     2881 ttcagttgat actgatacct gctgtggatt tcttcatcac cacaaccagc agatcgctga
     2941 tactggttgt tggtgtccag ttattcgctc ctgatgaaga tacggtgaat gtcagtgtca
     3001 gtgtcccctg tccggcaggc atatctataa ctgaggaaaa tacgccctga gcatccgtcg
     3061 tggactgatt aaaaatctcc tgaccattgc gggtcactct taaccggcag gttgaatacc
     3121 agtatgactg ttggttatta ctgttgaaat tctcatgctt accaccgcgg aataacactg
     3181 gcggtatcat gacctgccgg tcaaatttct gatcatcact gattcttacc gtgatggtgc
     3241 cactggcata actgttcgtg cgggggaaag acttgctgac cgttttgaca atatcacctt
     3301 caatctggtt ggctgacagt ttccccttaa tctgacagtt ctcattaatc gtgacgttgt
     3361 tgagcgtccc tgagttcgca ttcacactgc cactgatatc cgcattttta gcggtcagct
     3421 ttccgtctga tgtcagggaa aataccggag gactgccacc gctggtaatg gtgggtgccg
     3481 tcaggcgttt caggaacacg tcgttcatga atatctggtt gccctgcgcc acaaacatcg
     3541 gcgtttcatt cccgtttgcc gggtcaataa acgcgatacg gttagcggca accagaaact
     3601 ggctcagctt gccttcctcc gtatcctcca tactgaggcc aatacccgcg acataatgtt
     3661 tgccgtcttt ggtctgctca attttgacag cccacatggc attccattta tcgttggcat
     3721 ccttccactc tttcgaaaac tcatccagtc tgctggcgtt atcctccgtc agatctactt
     3781 tttccagcag ctctttaccg agatgggatt cggttatctt gcctttgaaa aaatccaggt
     3841 aaccttccgc atcatcgctc gcccgaccga cggcctccac aaatgccgat ttgccaacgg
     3901 tgttcacact gcggatataa aagtaataat catggcccgg tttgatattg atactggcgg
     3961 ctatccagta cagccccgtg ccaaggtagc gggctgtggt ttcaacctgc ctgatatcgg
     4021 taatccgcgt ttccgagaac cagaactcaa actgtacagt cggatcataa accgcaagat
     4081 gcggcgtggc ggttatctga aaatagcccg gcgtcagctc aatccgcgat ggcgctgccg
     4141 gtgcggcaat ccggaacgat accgacgccg gatcgccctg ctgcccccag gcatttaccg
     4201 cccggactgt cagactgtaa tttcccagag ccagctgcct gaagcggtat gtggtttccg
     4261 tcgtccgggc cgtgctgacc agccgctcac tgccgtcatc cgctgccacg gtcaggcgaa
     4321 gcataaagct cacgcccttc accaccttcg gcgtatccca gcgcgccagc acctggtatt
     4381 ccccgctgtc tgcggtgact tctgcggtca ggtgttgcac tgctggcgga gtgacaccat
     4441 tcaccgtgcc gctctggtcg ccgtcaaagt gcgccccgtt atccacgatg gcctctttct
     4501 ccggtacatg ctgcacggca gtgatggcat acgtgccgtc gtcgttctca cggatactca
     4561 cgcagcggaa caggcgctgg cgcaacgtcg gcaacttcag cccccacacg ctgtattcag
     4621 caacgccgtc gggaacacgg ctcactttca ccttcacgcc gtcggtgacg gactggacct
     4681 ccacgctgac cggattaccc tcaccgtcaa ccaggcttat cagcgtggtg ccggaggatg
     4741 gcagcgtgat ttcacggtcg agcgtcagcg tccgggtctg gctgtttacc gccagcacgc
     4801 gcccgccggt gctgataccg gcatagtcat catcacagat ttcaatgaca tcgcccggca
     4861 catggcgaag cccttctgcg cccacgctga agtccacggt ctgcgtttcc agcagttctg
     4921 ttttaatcag ccacagcccg gcgcggtgtg cctgcccccg gctggtacag ccaaaagcat
     4981 ccatcttcgt gacgttacga ccgtaacggg caatggcctg cgtatcctcc acaagctctg
     5041 tcgccgtctc ccagccgttg ttcgggtcaa tccagttcac ctcaacggca ttatggcggt
     5101 ctttcagggc gctgaagctg tagcggaacg gcgcgccatc atccggcatc accacattac
     5161 tgcggttata ggtccacacc ttatccgatg gccggtcctg cacaaacgtc agcgtctgcc
     5221 cgttccatac cggcatacag cgcatcgccg agcagaaatc actgagcaca tcccacgcct
     5281 tgcgctgcgt ggtcaggtac gcattacagg taatacgcgg ctccgtgcca ccaaagccat
     5341 ccggcaccga ctggtcgcaa ttctggccga tgacatacag cgcccattta tccacatccg
     5401 ccgcaccaag acgtttcccc atgccgtagc gcggatgggt cagcatatcc cacagacacc
     5461 aggccatgtt gttgctgtat gctggcttaa acgttccgtc ccagataccg ctgtattgcc
     5521 gcgtctgcgg gttatagttc gacggcacct gcagaatgcg tccgcgaaga tgataattac
     5581 ggctcacctg ctggctgccg aactgctccg agtccacctg cacgccgacc agtgccgtgt
     5641 tcgggtagca ctgtttcaca tcgatgattt cggtgtatga cgaccagagc gttttgttct
     5701 gcagctggtc tgtggtactg tccggcgtca tcctgcgcat ccggatatta aacgggcgcg
     5761 gcggcaggtt atccaccacc accgaggcca gatactgcga ggtggttttg cccttaatgg
     5821 tgatgtcttt ttccgtcacc cagccaccat tacgctgtat ctgaaccagc aggcgaactt
     5881 ccgacggatt ccggtccccc tttgaggtgg tttccaccag tgcctgcaca ccgaaggtaa
     5941 agcgcagacg gtcgatgttt gcagacgtga tggtccgggt gatcggcgtg tcgtatttca
     6001 cttccgtacc cagcaccgtc tcggatccgg aggattcaaa tccctccggc ggtgtctgct
     6061 cctgctcacc ggcccggaac accaccgtga caccggcgat
//
LOCUS       DAZFKF010000196         6047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFKF010000196 DAZFKF010000000
VERSION     DAZFKF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.198"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..282
                     /locus_tag="ACHKA1_005104"
     CDS             <1..282
                     /locus_tag="ACHKA1_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFO5682633.1"
                     /translation="EPACGAGSMILAMADTLNRSGYPAYHRMWVSATDIDPLAAGMAY
                     IQLSLCGVAGEVVIGNSLCNERRRVLLTPGHYLGNWDHRLRHLQEQNVA"
     gene            complement(516..1068)
                     /locus_tag="ACHKA1_005105"
                     /pseudo
     CDS             complement(516..1068)
                     /locus_tag="ACHKA1_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1185..2090
                     /locus_tag="ACHKA1_005106"
     CDS             1185..2090
                     /locus_tag="ACHKA1_005106"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO5682634.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGILNTPVCRSPFSP
                     VKEKNLDVLSKLAERLLEEHDRNKK"
     gene            2135..3010
                     /locus_tag="ACHKA1_005107"
     CDS             2135..3010
                     /locus_tag="ACHKA1_005107"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009384 - N-acylmannosamine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO5682635.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNELAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYIQKVSKHLSIYSEICNVMLFPAYFRSD
                     SGLIGATLWDRDCIT"
     gene            3059..4531
                     /locus_tag="ACHKA1_005108"
     CDS             3059..4531
                     /locus_tag="ACHKA1_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5682636.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAVLITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            4535..5365
                     /locus_tag="ACHKA1_005109"
     CDS             4535..5365
                     /locus_tag="ACHKA1_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5682637.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDSSLFENTDDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEESSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
     gene            5411..>6047
                     /locus_tag="ACHKA1_005110"
     CDS             5411..>6047
                     /locus_tag="ACHKA1_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFO5682638.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNTIHNNENELFALNYNKIDVNKTYKIDDKWALIPGIQTQWMS
                     AGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKIN
                     DSWLFAWQTTVYTKVNNFKYGNHKKTATENAFVLQYKMSPVFTPYIEYDYLD"
BASE COUNT         1659 a         1030 c         1485 g         1873 t
ORIGIN      
        1 gaacctgcct gtggtgccgg cagtatgatt ctggcaatgg ctgacaccct gaaccgttcg
       61 gggtatccgg cataccacag gatgtgggtg tcggcaacgg atattgaccc gctggcagcc
      121 ggcatggcgt atatacaact gtcgctgtgt ggtgtggccg gagaagtggt gattggtaac
      181 tcgctctgta atgagcgacg ccgcgtactg ctgactcccg ggcattacct gggaaactgg
      241 gaccatcgtc tgcgtcatct tcaggagcag aatgtggcat gatgcagaat gttttatcac
      301 tgcgccagtc ttcggactgg cgttttgttc aactgccagt tccctgaatg tttgatttgt
      361 tcaataaagc ctgtttatca ataaattttc tgacgacagg gaatggtatg tttttattag
      421 tttttatgtg ttggacaaca agacagaata atgatgtctg ttttatgtat acgatatagc
      481 cactgattca acgcattatc ggctatattg ccagtcttat ttagtgccag attcattgta
      541 tcgaaggccg ctgccgtcag tagatggctg gtcagttcta tcagacagac catttttacc
      601 tgaggataaa gtgtctggtt tccaccatgt atttggcgag tgaagcttta tcgtgttctt
      661 gcgtgtcctg tgttcgtcag aacacgccat caatggtcag cagattcaga tcgcaccaga
      721 gcggatatgg cgtggtgtta tgccagagct gcgctgtttg agtgaacacg cagcgtatgg
      781 ctccgcttcc tggccgctgg cgggcatgga gcacggcgct actgcctggc agtatgatgt
      841 ccagccggtt cacaatctga tgaaggttcc ttaagctcat gggccatact gataattcac
      901 cagaccatca tttccagagg aagacgccgt ttgtgtagcg tgacagtgtc ggactcttca
      961 gggctgtggg taatcagttc cagggcaaga tagtcgcaga gggttacgca gagaatagta
     1021 actggacact ggatcgatca accgatcctt aactgagcgg catgacagct ttctgatgga
     1081 tttttttgat aatcttcaca ttgtgcttct tttctgttgg ataatttttg cacttggtgg
     1141 tgtactaggt atcccattat gcagttaagt gaaggtgatt aaaaatgcaa tgtgaattta
     1201 aaggggtcat atcagcatta cccactcctt atgaccaatc ccagcaaatc gatatggaga
     1261 gtcttcgaaa actgatccgt tttaatatcg aacaaaatat taagggattg tatgttggag
     1321 gctctactgg agaagcattt cttcagaatg ttgcagagcg agaaaaaatt cttgaaacag
     1381 tagccgatga atcagatgga agactgacac ttatagcgca tgtgggcgga atcagtacgg
     1441 cagaaagcga ggtacttgca aaagctgcaa aaaaatacgg atatcatgct atatctgctg
     1501 ttactccatt ttattatcct ttttcatttg aagagcattg tatccattac aggaagatta
     1561 ttgattctgc agatggactt ccgatggtag tttataatat accggctctt agcggagtgc
     1621 gtttttcttt agatcagata aatgagcttg tcacaatacc cggggtttgt gcattaaaac
     1681 agacatccgg tgatttattc cagatggaac aaatcaaaag gaatcatcca gagcttgtgt
     1741 tatataatgg ttacgatgaa atatttgcat cagggttaat tgcaggtgca gatgggggaa
     1801 tcggtagtac atataatata atgggatggc gttatcttga aatatttgaa gcagtcaaaa
     1861 acaatgatgt gattaaggcg aaggaaatgc aggtggcctg taaccaggtt attgacactc
     1921 ttattcagag tggtgtcctg gcaggtataa aaactcttct ttactatatg gggattctaa
     1981 atactcctgt atgcagaagt cctttttctc cggtaaagga aaagaatctg gatgtattat
     2041 caaaacttgc agagcgactg ctcgaagaac atgacagaaa taaaaaatga aaataatctg
     2101 aataattgca agattgttat ataggtgaaa atgaatgata acactggctg ttgatatagg
     2161 aggaacgaaa atttcagcgg cgttgatatc tgatgacggt tcatttttgc tgaaaaaaca
     2221 gatatcaaca cctcatgaaa gatgtcctga cgaaatgaca ggggctttac gtctactggt
     2281 ttctgaaatg aagggaacag cggagcgatt tgcagtagca tcaacaggga ttattaataa
     2341 tggagtattg acagctctga atccagacaa tcttggaggg ctgaaagagt atcctctgaa
     2401 aaatataatg gaagatataa ctggtttaaa tggaagtgtg ataaatgatg cccaggcagc
     2461 cgcatgggca gaatatacag ttttgccaaa agaaatttgt gatatggttt ttataaccgt
     2521 ttctacgggg gttggagggg gaattgtagt taacagaaaa ttattaacag gtgtttctgg
     2581 attagccgga catgttgggc atatattgtc cggagtaacg gatacagaat gtggatgtgg
     2641 tcgtcgggga tgcgttgaag cggtttcatc tggtcgggcc atcatggggg cagcaaaaaa
     2701 tgaacttgca ggatatagca ccaaatatat ttttgaatta gcgagacagg gatataagga
     2761 agcagaattt ttgacagagc gttctgccag tactattgct gaattaatag taagtttaaa
     2821 attactgctt gattgtcagg ttgttgttgt cggaggaagt gttggacttg ctgatgggta
     2881 tattcagaaa gtgtcgaaac atctttccat atattctgaa atttgtaatg taatgctgtt
     2941 tcccgcatat ttcagaagtg actctggtct tattggtgca actctttggg accgggattg
     3001 tattacttaa agtaatactc cagtgtatta taaatccgct attattgtga ggtttaatat
     3061 ggactcctgt attcagaaaa agcaggtttg gtataagcat ctttcaccca gacagtggaa
     3121 gatattcggt gcggcatgga caggttattt acttgatggt tttgatttcg ttctgatatc
     3181 tctggttctt acagaggttc agcatgaatt cggcttgacg acaatagaag cggcaagcct
     3241 tatttccgct gcttttattt cccgatggtt tggggggctt gctattggcg ctttatcgga
     3301 caagatgggg cgcaggatgg caatggtttt aagtatagtg ctattctctc tcggaaccct
     3361 tgcctgtggt ctggctcctg gatatgcagt gatgtttatt gctcgcattg ttattgggct
     3421 tggtatggca ggtgaatatg gctcaagtgt aacatatgtt atcgaaagct ggcctgttca
     3481 tctcagaaac aaggcaagtg gttttttaat ttctggattt tctattgggg gaggtttagc
     3541 tgcacaggtt tatagcattg tagtaccact atggggctgg cgttctcttt tctttgtcgg
     3601 catgcttcca atattatttg ctttttatct tcgcaagaat cttccggagt ctgatgactg
     3661 gcagaaaagg caacaagaga ataaacccgt cagaacaatg gtggatattc tgtacagaga
     3721 aaagaataaa tacataaata ttcttctgtc ttgtattgca tttgcatgtc tatatgtttg
     3781 tttttcaggc gtcactgcaa acgctgtttt aatcacagtt atggcattgt gctgtgcggc
     3841 tgtttttatc agttttatat atcagggtat ggggaaaaga tggccaacag gtattatgtt
     3901 gatgcttgtg gttatgttct gtttcctcta cggatggcca ctgcaggctt ttcttcctac
     3961 atggcttaag gttgatatgc agtattcacc agaaactgtt gctctcatat ttatgctggc
     4021 aggttttggc tctgcagctg gaagttgtat aggtggtttt atgggggact ggcttggaac
     4081 ccgaaaagca tatgttatat cacttcttat cggtcagctt gttattatcc ccgtttttct
     4141 tgtggaccgg gactatgtat ggttacttgg ccttctgatc tttactcagc aggtttttgg
     4201 tcaggggatt ggtgctctgg tgccgaaaat aatcagtggt tattttaatg tggagcagcg
     4261 tgcggcagga ctggggttta tttataatgt aggttcactt ggtggagcat gtgcgcctat
     4321 tcttggtgca gttgttgcgt ctcatacaag tcttggtaca gcaatgtgtt ctcttgcttt
     4381 tatcttaaca tttgttgttc ttgtccttat tggttttgat atgccatcac gtgtacagag
     4441 atggatccat ccagaagctg cgcttgaata tgatactgtc gatggaaaac ctttttatgg
     4501 agcaagaaaa aagaatgttg caggagaata acggatgcta tcagggaata aaaaaatgat
     4561 tcattacagg gagaatatat attctcttga taattatgag cgttacataa ctgacagttc
     4621 gctatttgag aatactgacg atggcataat atatacaggg attgtgcccc ttattacagt
     4681 tgttgagcct gttgtatcta atcaacatag tgtgctgatt atacctggag gtggatacag
     4741 gaggattgtt tatgaaagag agggacttga tgtaggcaga tggatagcgg gtcagggata
     4801 tacggcattt attctttctt acagaatgcc ctccgttaat ggttattatt cctttgcgga
     4861 tgcgaagaat gccctgaact ttatttctta ctgggccgga atgtcggaga gaacatttaa
     4921 gagtgttgga tgtatggggt tttcggcagg tggtcatctt gctggatata ttactcatct
     4981 ccattatgag gaagagagtt ctgtgcgtcc tgactggatg gctctgatat atccagttat
     5041 atctcttgac aggagtattg ctcatcaggg atgccggaaa cgtcttctgg atgcaggtga
     5101 aagtgaatat tcagccagtc ttgaaaatag tgttacagag tatatgccac cagtctttat
     5161 tgtgcatgca acagatgata aggatgttcc agttattcat tctctgagat attttaactc
     5221 tatccgggat aagggacatc atgcagaagc tcattttttt gataagggag gtcatggatt
     5281 tggtgtatcc ggaaaaaatg gatatccgat agaaaaatgg caaagcctgc ttctggaatg
     5341 gcttgcggaa cataagtttc attgagaaat cagattacaa gtgataaata tatttaatga
     5401 tgaggcattt atgaaaaaat attatccact attaactgta attcttcttg gtttaccttt
     5461 gtgctctcag gcattaactc ttaatctacg tggaggatac agaagcgcca gtcacgctta
     5521 tgaaacgttg attcaggtta gtcaggggtg gaataatggt tggtgggcaa gttttgagtc
     5581 aaacacctgg aatacaattc acaacaatga aaatgaattg tttgctctca attataataa
     5641 aatagatgta aataaaacat ataaaataga tgacaaatgg gctctaatcc cagggattca
     5701 gacacaatgg atgtcagcag gaagtcagta taacccttat ctgaaaattt cgtatcaggc
     5761 aacgccagat cttaatctgg ccttacgcta tcgctatgac tggaaagctt tccgacagac
     5821 ggatttagat ggcaacaggg cgcgtaatga tcagcatcag tttgatggtt atgtgacata
     5881 caaaattaat gattcatggt tgtttgcatg gcagaccacg gtatatacga aggttaataa
     5941 tttcaaatat gggaatcata aaaaaacagc aactgaaaat gcatttgttc tgcaatataa
     6001 aatgtcaccg gtgtttaccc catatataga atatgattac ctggata
//
LOCUS       DAZFKF010000197         5961 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFKF010000197 DAZFKF010000000
VERSION     DAZFKF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.199"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..344
                     /locus_tag="ACHKA1_005111"
     CDS             <1..344
                     /locus_tag="ACHKA1_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFO5682639.1"
                     /translation="QLRRAIASVNGNQFRGGNDYQIDQWNDCARLIANCVIYYNSAIL
                     SRLVEKFEKENNKKAIDVLANLSPVAWRHILLGGNYSFEDQVAITNLDSLLEGVDPLS
                     DIGDIESEYDG"
     gene            349..738
                     /locus_tag="ACHKA1_005112"
     CDS             349..738
                     /locus_tag="ACHKA1_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5682640.1"
                     /translation="MINNGFYAKSETFGGNGRRTPKTVSIECTGMGDERTQKVDASFT
                     GTNRSSDDTMVATDNADVGIKIYNKSNAEVSVNNGKLPADMGNTTIIGRKNGSVTFSA
                     APASFTGARPQPGVFNATATLTIEFVN"
     gene            950..1990
                     /gene="pstS"
                     /locus_tag="ACHKA1_005113"
     CDS             950..1990
                     /gene="pstS"
                     /locus_tag="ACHKA1_005113"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO5682641.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            2077..3036
                     /gene="pstC"
                     /locus_tag="ACHKA1_005114"
     CDS             2077..3036
                     /gene="pstC"
                     /locus_tag="ACHKA1_005114"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO5682642.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            3036..3926
                     /gene="pstA"
                     /locus_tag="ACHKA1_005115"
     CDS             3036..3926
                     /gene="pstA"
                     /locus_tag="ACHKA1_005115"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO5682643.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            4097..4870
                     /gene="pstB"
                     /locus_tag="ACHKA1_005116"
     CDS             4097..4870
                     /gene="pstB"
                     /locus_tag="ACHKA1_005116"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO5682644.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            4885..5610
                     /gene="phoU"
                     /locus_tag="ACHKA1_005117"
     CDS             4885..5610
                     /gene="phoU"
                     /locus_tag="ACHKA1_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO5682645.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
     gene            complement(5757..>5961)
                     /locus_tag="ACHKA1_005118"
     CDS             complement(5757..>5961)
                     /locus_tag="ACHKA1_005118"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682646.1"
                     /translation="GCWFETIMAKPPMNTVKNRSLCMFRYRAFTLFFVFFSILCAGSD
                     INEKNNKNVFIAWHYIKSKIIQM"
BASE COUNT         1470 a         1438 c         1564 g         1489 t
ORIGIN      
        1 atcaactaag aagagcaata gcatcagtaa atggaaatca atttcgcggt ggtaatgact
       61 accaaataga tcaatggaac gactgtgcga ggttaatagc taattgtgta atctattata
      121 attcagcaat attatccagg ctggtcgaga aatttgaaaa agaaaataat aaaaaagcga
      181 tagatgttct ggctaactta tccccagtag catggagaca cattctcctt ggagggaatt
      241 attcttttga agatcaagtt gcgatcacaa atttggacag tcttttagag ggagtagacc
      301 cactaagtga tatcggagat attgagtccg aatatgacgg ttaacaacat gataaataat
      361 ggtttttatg ctaagagtga gacttttgga ggaaatgggc gcagaacccc taaaacagtg
      421 agtattgagt gtacggggat gggggatgag cgtacgcaga aagtcgatgc ttcttttacg
      481 gggacgaacc gaagcagtga cgatacgatg gtggcgacag acaatgctga tgtcgggatc
      541 aaaatttaca ataaatcgaa tgctgaagtt agcgtcaaca acggcaagtt acccgcagac
      601 atgggcaaca cgaccattat tggtcgtaaa aatggttcgg taactttttc ggcagcacct
      661 gccagcttta ccggtgcccg gcctcagccc ggcgttttta acgctaccgc gaccttaacc
      721 attgaatttg taaactaacc ttttgcggct gttttatgac agccgttcat atttcctctc
      781 ctttcatgaa aaaatgctga tatttttccc cttcaatatc aataagtaat aactttcctt
      841 tgggtgtcat caaactgtca cattccttac atataactgt cacctgtttg tcctattttg
      901 cttctcgtag ccaacaaaca atgctttatg aatcctccca ggagacatta tgaaagttat
      961 gcgtaccacc gtcgcaactg ttgtcgccgc gaccttatcg atgagtgctt tctctgtgtt
     1021 tgcagaagca agcctgacag gtgcaggtgc aaccttccct gcgccggtgt atgccaaatg
     1081 ggctgacact taccagaaag aaaccggtaa taaagttaat taccagggta tcggttcttc
     1141 cggtggcgta aaacagatta tcgccaatac cgttgatttt ggtgcttctg acgcgccgct
     1201 gtctgacgaa aaactggctc aggaaggtct gttccagttc ccgaccgtga ttggcggcgt
     1261 ggtgctggcg gttaacattc ctgggttgaa gtctggcgag ctggtgctgg atggtaaaac
     1321 cctcggcgac atctacctcg gcaaaatcaa gaagtgggat gatgaagcca tcgccaaact
     1381 gaatccgggt ctgaaactgc cttcacagaa cattgctgta gtacgccgcg cagatggctc
     1441 cgggacttcc ttcgtcttca ccagctacct ggcgaaagtg aatgaagagt ggaaaaacaa
     1501 cgttggtact ggctctaccg taaaatggcc gatcggtctg ggtggtaaag gtaacgacgg
     1561 tatcgctgcg ttcgttcagc gtctgccggg tgcaattggt tacgttgaat atgcttatgc
     1621 gaagcaaaac aacctggcgt acaccaaact gatctccgct gatggtaaac ctgttttgcc
     1681 gactgaagaa aacttcgcta atgcagcaaa aggtgcagac tggagcaaaa ccttcgctca
     1741 ggatctgacc aaccagaaag gcgaagatgc gtggcctatt acctctacca cgttcattct
     1801 gatccacaaa gatcagaaga aaccagaaca aggcacagaa gtgctgaaat tcttcgactg
     1861 ggcgtacaaa accggggcta aacaggcgaa cgacctggat tacgccagcc tgccggatag
     1921 cgtagttgaa caggttcgcg ctgcgtggaa gaccaatatt aaagacagta gcggcaagcc
     1981 gctgtactaa taaaactcca ggccggataa ggcattaaac ggcttatccg gcattacaaa
     2041 atgactttgt aaacgcgttt aactgaagag taacttatgg ctgcaaccaa gcctgctttt
     2101 aacccaccgg gtaaaaaggg cgacataatt ttcagcgtgc tggtaaaact ggcggcgctg
     2161 attgtgctat tgatgttggg tggcattatt gtctctctga tcatctcctc atggccgagc
     2221 attcagaaat ttggtctggc tttcctgtgg accaaagagt gggatgcgcc gaacgatatc
     2281 tacggggcgc tggtgccgat ctacggtacg ttggtgactt cgtttatcgc gctgctgatc
     2341 gccgtcccgg tgagtttcgg tatcgccctg ttcctgactg agcttgcgcc tggctggctg
     2401 aaacgcccgc tgggtatcgc cattgagctg ctggcagcca ttccaagtat cgtttacggc
     2461 atgtggggcc tgtttatctt tgcgccgctg ttcgccgttt acttccagga gccggtcggc
     2521 aatatcatgt cgaatatccc gattgtcggc gcgctgttct ctggcccggc atttggtatc
     2581 ggtatcctcg cggcaggcgt gatcctcgcc atcatgatta ttccgtacat tgcggcggta
     2641 atgcgcgatg tgttcgaaca aaccccggtg atgatgaaag agtcggccta cggtattggc
     2701 tgcaccacct gggaggttat ctggcgtatc gttcttccgt tcaccaaaaa tggtgttatc
     2761 ggcggcatta tgctggggct gggccgcgcg cttggtgaaa ccatggcggt gacctttatc
     2821 atcggtaaca cctaccagct cgacagcgcc tcgctgtaca tgccgggcaa cagtatcacc
     2881 tctgcgctgg cgaacgaatt tgcggaagcg gaatccggtc tgcacgttgc cgcactgatg
     2941 gaactgggcc tgatcctgtt tgtgattacc ttcatcgtcc tcgccgcatc gaagtttatg
     3001 attatgcgcc tggctaagaa tgagggggca cgctaatggc tatggttgaa atgcaaacca
     3061 ctgcggcgct tgctgaatcc cgccgcaaaa tgcaggcgcg tcgccgcctc aagaaccgta
     3121 ttgcactgac gctctcaatg gcgacgatgg ccttcggtct gttctggctg atctggattt
     3181 taatgtccac catcactcgc ggtatcgatg gtatgtcgct ggctctgttc actgaaatga
     3241 cgccgccgcc caatacggaa ggtggtggtc tggcgaacgc tctggcgggt agcgggctgt
     3301 taattttgtg ggccacggta ttcggtacgc cgctgggcat tatggcgggg atttatctgg
     3361 ctgaatatgg tcgtaaatcc tggctggcag aagtgattcg cttcattaac gacattctgc
     3421 tctctgcgcc gtcgattgtg gttggtctgt ttgtttacac catcgtggtg gcgcagatgg
     3481 agcacttctc cggttgggcg ggtgtgattg cgctggcgct gttgcaggta ccgattgtta
     3541 tccgcaccac cgagaacatg ctgaaactgg tgccggacag cctgcgtgaa gcggcttatg
     3601 cactgggtac gccgaagtgg aagatgatct ccgcgattac gctgaaagcg tcggtgtccg
     3661 gaattatgac cggtatcctg ctggcgattg cccgtattgc cggtgaaacc gcgccgctgc
     3721 tgtttaccgc gctctccaac cagttctgga gcacggacat gatgcagccg atcgccaacc
     3781 tgccggtgac gatctttaag tttgcgatga gcccgtttgc ggaatggcag caactggcct
     3841 gggccggggt attgatcatt accctgtgcg tactgctgct gaacattctg gcgcgcgttg
     3901 ttttcgcgaa gaataaacac ggttgatatt gctgacacgg ttttcccctc tccccagagg
     3961 ggcgagggga ccgaccgagc gcctttttga ctctgtacac ggttaacact ttgccggatg
     4021 cggcgtaaac gcctgatccg gcctacggta agcctgatta gcgaagtgca tcaggcacga
     4081 tgaggaaaag attgcaatga gtatggttga aactgccccg agtaaaattc aggttcgtaa
     4141 tttgaacttc tactacggca aattccatgc cctgaaaaac atcaacctgg atatcgctaa
     4201 aaaccaggta acggcgttta tcgggccgtc cggctgcggt aaatcgacgc tgctgcgtac
     4261 cttcaacaaa atgtttgaac tgtatccgga acagcgtgcg gaaggtgaaa ttctgcttga
     4321 tggcgacaac atcctgacca actctcagga tatcgccctg ctgcgtgcga aagtgggcat
     4381 ggtgttccag aagcctacgc cgttcccgat gtccatttac gacaacatcg cctttggtgt
     4441 ccgtctgttt gagaagctct cccgtgccga catggacgag cgcgtacagt gggcattgac
     4501 caaagccgca ttgtggaacg aaaccaaaga taaattgcac cagagcggtt actctctctc
     4561 tggtggtcag caacaacgtc tgtgcattgc gcgtggtatc gccattcgcc cggaagtgct
     4621 gctgctcgac gaaccgtgtt cggcgctcga ccctatctct accgggcgta ttgaagagct
     4681 gatcaccgaa ctgaagcagg attacaccgt ggtgatcgtc acccacaaca tgcagcaggc
     4741 tgcgcgttgt tccgaccaca cggcgtttat gtacctgggc gaattgattg agttcagcaa
     4801 cacggacgat ctgttcacca agccagcgaa gaaacaaaca gaagactaca tcaccggtcg
     4861 ttacggttga ttcaggagtg cgttatggac agtctcaatc ttaataaaca tatttccggc
     4921 cagttcaacg ccgaactgga aagtatccgc acgcaggtga tgaccatggg cggcatggtg
     4981 gagcagcagc tttctgatgc aatcaccgcg atgcacaacc aggacagcga tctggcgaag
     5041 cgcgtcatcg aaggcgacaa gaacgtcaac atgatggaag tggcgatcga tgaagcctgc
     5101 gtgcgcatta ttgccaaacg tcagccgaca gcgagcgacc tgcgtctggt tatggtgatc
     5161 agtaaaacca ttgctgaact ggagcgtatt ggcgacgtgg cggacaaaat ctgccgtact
     5221 gcgctggaga aattctccca gcagcatcag ccgttgctgg taagtctgga gtcgctgggc
     5281 cgtcatacca tccagatgct gcacgacgtg ctggacgcgt tcgcgcggat ggacattgac
     5341 gaagcggtac gtatttatcg tgaagataaa aaagtcgatc aggagtatga aggtattgtt
     5401 cgtcaactga tgacctacat gatggaagat tcgcgtacca ttccgagcgt acttaccgcg
     5461 ctattctgtg cgcgttctat cgaacgtatt ggcgaccgct gccagaatat ttgtgagttt
     5521 atcttctact acgtgaaggg gcaggatttc cgtcacgtcg gtggtgatga gctggataaa
     5581 ctgctggcgg gaaaagaccc caaagagtag tgctaactgt aggccagata aagctttttg
     5641 ccgcatctgg caatcagccc ggtcatacat agttaccggg ctgataaact ttctcactga
     5701 aatattgcca cacaaactca cctcgaaact ctccccagta cattgcatat acattattac
     5761 atttgaatta ttttactttt aatgtagtgc cacgctataa aaacattttt gttattcttt
     5821 tcatttatat cgctacctgc acagagaatg gaaaagaaaa caaaaaacag ggtgaaagcc
     5881 ctatatcgaa acatacataa tgagcgattt tttacagtat tcataggtgg tttagccata
     5941 atcgtctcaa accaacaccc a
//
LOCUS       DAZFKF010000198         5906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFKF010000198 DAZFKF010000000
VERSION     DAZFKF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.200"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            245..940
                     /locus_tag="ACHKA1_005119"
     CDS             245..940
                     /locus_tag="ACHKA1_005119"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4868 domain-containing protein"
                     /protein_id="HFO5682647.1"
                     /translation="MNKKHFYTEGVEFSGGYITSGDEFFVIPDFDDVIAVLDAINNPT
                     SIPPWEPEEISAFNIIALFSGYPEEDGKPVTALIQSFDKRQVIDNRRTILQKMFQASN
                     TFCQSTEHGIVIDNKLSAILAGTELKFKSFHMLRRIFDVDAYFREATNEELTSFSSHE
                     KFSVVPGFDLTTIADSVIRKKVSLINKSRILEDYSVTELRISASEIGVALEAEKIGEI
                     EKIKMPQIRKDVN"
     gene            1469..2512
                     /gene="subA"
                     /locus_tag="ACHKA1_005120"
     CDS             1469..2512
                     /gene="subA"
                     /locus_tag="ACHKA1_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFO5682648.1"
                     /translation="MLKILWPHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKESEALYIHGTAMASLIASRHR
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWTELLSRMGRNNERLIVAAVGNDGADIRKLSAQQRIWPAAYHPVSSVNKKQDPVIRV
                     AALAQYRKGETPVLHGGGVTGSRFGNGWVDIAAPGQNITFLRPDGKTGIGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYSSLADKVTEGRVLNAEKAISMFCKKN
                     YIPVRQGRMNEEL"
     gene            2529..2951
                     /gene="subB"
                     /locus_tag="ACHKA1_005121"
     CDS             2529..2951
                     /gene="subB"
                     /locus_tag="ACHKA1_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFO5682649.1"
                     /translation="MTIKKFFVCAGVMGCLSLNPAMAEWTGDARDGMFSGVVIDQFHT
                     GQIDNNPYFCIEGKQPGGSSIRACSMKNSSVWGPSFSTLYNQALYFYTTGQLVRIYYE
                     PGVWTYPPFVKALTSNALVGLSTCATSTECFGPDRKKN"
     gene            complement(3267..4253)
                     /locus_tag="ACHKA1_005122"
     CDS             complement(3267..4253)
                     /locus_tag="ACHKA1_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO5682650.1"
                     /translation="MYRHDFNEKIDFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRV
                     KQLARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHI
                     AKKLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLV
                     ARVKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCV
                     GNSSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEP
                     EEDADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            4353..5087
                     /gene="nanR"
                     /locus_tag="ACHKA1_005123"
     CDS             4353..5087
                     /gene="nanR"
                     /locus_tag="ACHKA1_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO5682651.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILADIPDNKVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEIL
                     TAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(5123..>5906)
                     /locus_tag="ACHKA1_005124"
     CDS             complement(5123..>5906)
                     /locus_tag="ACHKA1_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5682652.1"
                     /translation="FELDRVLTDIYAPQLIIFLPDKPNGTGILIIPGGGYQAVMLDKE
                     GTALAPEFLSQGYTLFVLSYRLPSPAEEENINKTFIDAISAMLYIRKNADKWHLQRHR
                     IGVLGFSAGGHIASTFVNQICDFKRSSDSKEQEIFAIPDFVGLIYPVISMKDDVTHPG
                     SRKQLLGDNSTSENIIQYSADLNVTSCFPPVFLLHCCDDDLVSIENSLLMYRGLIEKG
                     VSAEMHLYEKGGHGFSVQWATDLPVGGWIKLFMDWMYSHGFK"
BASE COUNT         1813 a         1210 c         1201 g         1682 t
ORIGIN      
        1 ttggggatat aaatattatg aagctgaaga cgatacaggt gtaagctttg tcatattatc
       61 taaaaaacca ctgctaaaag catcaggtaa tcgtgttaat ttgattaagc ttactgatta
      121 cacatatata gtaatacagg agtaagtatg gaattatttg caataacaga taatacagtt
      181 ggcacacgta ttgtcagaat tgttactgac agaccgactc aaaatgtagt cacacagtta
      241 ttcagtgaac aaaaaacatt tctacacaga aggtgtggaa ttttcaggtg gatacatcac
      301 tagtggagat gagttttttg tcattcctga ctttgatgat gttatcgctg tattagatgc
      361 cattaataat cccacatcta tccctccatg ggagccggaa gaaatatcag ccttcaatat
      421 aatcgctctt ttttcaggct atcctgaaga ggatggaaaa cctgtaactg cattaattca
      481 aagcttcgat aaaagacaag ttattgataa cagaaggaca attttacaaa aaatgtttca
      541 ggcaagcaat actttttgcc agtctactga gcatggtata gtcattgaca ataagctaag
      601 cgctattctc gcaggtacag aattaaaatt taaaagtttc catatgctcc gcagaatatt
      661 tgatgtagat gcttatttca gagaggcgac taatgaagaa ttaacaagtt tttcttctca
      721 tgaaaaattt tcagtagttc cagggtttga tttaacaaca attgctgact ctgttattag
      781 aaagaaagtt tccttaatta ataaatcaag aatacttgaa gactactctg tgactgagtt
      841 aagaatttca gcgtctgaaa ttggtgttgc tctagaagct gaaaaaatag gtgaaattga
      901 aaaaataaaa atgccacaaa taagaaaaga tgtaaattaa ggaaacaaca tgttgggtgg
      961 agcggacaat ccaaatggcg aattaccgtc ttatatcact ggcgctgaca cctgatatcc
     1021 ggggcttact gcacacgtaa taatgagcca gggtaaacga cacaaccatg cgctgatcgc
     1081 tccggtaaga cggcactgta atttcctgtt cgccatgatg catgacggta ctctttattg
     1141 tcataaacat gccaggcaac atagttgccc cctcttattt tttctggcta atttttaccc
     1201 atcatagctg gatttacttc tgctctgaag gagtaggaag agcatctcac cgattaccta
     1261 ttgcagaaat cctggtgcat attaccgtga aggagttcag tgagctattg aatatgcaaa
     1321 taaataaatt aatatttttg agaatgactt cactcgcttt attgattgtg attgtttatc
     1381 ttttgtcgaa aacaggatat ttcagatgtg atgttcctgt aagatattga cctgcaatac
     1441 tgtttatttg tactgactgg aggatattgt gcttaagatt ttatggccgc atattctgtt
     1501 cctgcttttt atatctgtgt ctgtcagggc agaaaaaccc tggtattttg atgcaattgg
     1561 cctgacagaa acaaccatgt cgctcacaga caagaatacc ccggttgtcg tatctgttgt
     1621 tgattccgga gtggcatttg taggaggcct cagcgacagc gaatttgcca aattcagttt
     1681 tactcaggat ggttcgccat tcccggtaaa agagtctgaa gctttatata ttcatggtac
     1741 tgccatggct tcactcattg cctcacgtca tagggtttat ggggtttatc ctcatgcact
     1801 gatatccagc agaagggtta ttcctgacgg tgtacaggac tcatggatta gagcaactga
     1861 aagcattatg tcaaatgttt ttcttgcacc aggagaagag aaaatcatta atatatcggg
     1921 aggccagaag gggatatctt ccgcatcggt ctggaccgaa ctgctttccc gtatgggccg
     1981 gaataatgag cgattaattg ttgcggcagt gggtaatgat ggcgcagata tacgaaaact
     2041 gagtgctcag cagagaatat ggccagcggc ttaccatccg gtcagcagtg tgaataaaaa
     2101 gcaggatcct gtgataagag tggcagctct ggcacagtac cggaaaggag aaacacctgt
     2161 attgcatggt ggaggggtca ccggaagtcg gttcggaaac ggctgggttg atattgccgc
     2221 accgggacag aatattacat tcctcagacc agatggaaaa acggggatcg gtagtggaac
     2281 ctcggaagca acagctatag tttccggcgt tctggcagcg atggtctcat gtaatccccg
     2341 tgcaacagcg acagaactga agcgtacact gctggagtct gcagacaaat actcctcact
     2401 tgcagataag gtgacggaag ggagggttct taatgcagaa aaagcaatta gcatgttctg
     2461 caagaaaaat tatattcctg tccgtcaggg aaggatgaat gaagaactgt aaaacttcgg
     2521 aggtgaatat gactattaag aagttttttg tatgtgcagg tgtcatggga tgtttatccc
     2581 ttaatcctgc catggcagag tggactggcg atgcccggga tggcatgttt tcaggcgttg
     2641 ttattgatca gtttcataca gggcaaatag ataataaccc ttatttttgt attgaaggga
     2701 aacaacctgg tggttcttcc ataagagcct gctcgatgaa gaattcatct gtctgggggc
     2761 cttcgttttc cacattatat aatcaggcac tatattttta cacaacaggt cagttggtca
     2821 ggatttatta tgaacccggg gtatggacgt atccaccctt tgtgaaggca ttaacgtcca
     2881 atgcccttgt tgggttatcg acctgcgcca catcgactga atgttttggc cccgacagaa
     2941 aaaagaacta ataggtgatg tttccgtcca gagatactct ggatggagtg tcggcgtcag
     3001 tgatataaga ctcgagtgaa cactgagccg acctcgaaat gacgttgtag gtgcagacat
     3061 gccataatgg gactgtcgat gatattacag ggagtcacgt cccctggtgc gtgccttcgt
     3121 gacacactac cgtccacata gtatcagcac cagtgatatc aaacggtaac gaccagctat
     3181 cctggtgttg ataccgacaa ctccatacca ctccgtcatg tctctcagag aatcatgtca
     3241 gagctgtaga tatatcctga acttgtctac aaataatttc ttattttttg cgcataacat
     3301 tcagcaataa ctccccttct ggccattgaa ttaaagtgtg tacttctctg tgacgttgtc
     3361 cagcttaatt ctgtcctcca tgatgacccc agatacccga cagataaatc atcggcatct
     3421 tcttctggct cattagttaa ttcatgtcgg ttactatcct gaaagctgag aaaatgtatt
     3481 tcatcatcag tccgttgtct gtaatgtcca tatataaaat catattcttt ttgataagat
     3541 tccttccagt accaggttgt atcaccacac aaccatggaa ctctttctga cgaattacca
     3601 acacactgag atgagtattc agataactca gacctgaaag aagttaccat ctgataaaac
     3661 aagtcaggat gcttttcata atctggtgac ataagatcaa actccccctg catccagcag
     3721 acagatagca gtttattctt tctgttatat tccagagcta ccttaacccg ggctactaag
     3781 tcctcataga gtgctgttcc ggtgccccac cgcattgcat cagctgttgc ccctgtatca
     3841 ggtacataca taccttcagc tccaacagta aaggcggcac ctcctctgca acaaggaacc
     3901 agtaatatcc ctgcttgctc aggtatgtat ggcagtaact ttttagctat atgcaatccc
     3961 tgtccgacac aaccatattc ccctttatgc aaatcagcag ccggatggtg atatccactc
     4021 atatcctgaa catcatgcag gcagtgatct gccggaataa tctcattgaa tttacactct
     4081 tttcctcctg gtgtgattgt ttttcgacga gccagttgtt ttacacggga ctcgggtttg
     4141 tcgagtgtat ctggcagggg aataccttca ccgtaggcca taccattaga ctgtcctgcg
     4201 agggctatca cataccagaa atctattttt tcattaaaat catgacgata catcactccc
     4261 cctttacata atagatttaa ttaaatattt tcattattaa aagaaagtgt taaattaaaa
     4321 actcatttgc tctaatccat ataaatcaat atatgtccga caacaaaaaa accgtgttac
     4381 ccgggctaac aacgaccaga aagcccattg ttacaaaaaa actttcagaa atcgttgccg
     4441 aagagcttga aagaatgatc cgccaggagg agataaggga aggtgaatac ctgccttctg
     4501 aacgtgaact tatggcattc tttggcgttg gaagaccatc tgtcagagag gctttatcat
     4561 ctctcagaca taaagggcta atccggtata acaagggaga aaaagccagt gtctgccgtc
     4621 catcaccaga aaccatcatt aatgggttat caggcattgt taaagatttt ttatccacag
     4681 ctgatggcat caaggatttt gaaagactac gcatcttttt tgaaaccaac cttgtaagat
     4741 atgcagcaaa aaatgcaacg cctgaggata ttcacaggct tgagaaggtg ctcagtatcc
     4801 atcataactc actgagaacc cccgaagtgt atgctcaata tgatattgaa tttcatagta
     4861 tacttgctga tatacctgac aataaagtat taaaaacaat acactcagca ttttctgaat
     4921 ggctgataat ggccaaacct ctggaaaaga ataaaatgac atatgaaaat aacaaaaaaa
     4981 cccatcagca gcatattgaa atacttacgg ccataaaaga gcatgattgt gataaagctg
     5041 aacagctaat gtataatcac ctttataaac gaattgaagc atcatagtta ctcgacgctt
     5101 caccaggtag tcaccataaa tattatttaa atccatgaga atacatccag tccataaaca
     5161 gttttatcca tcctccaaca ggaagatctg tggcccactg aacactaaag ccatgccctc
     5221 ctttctcata gagatgcatt tcagcagaaa cgcctttctc tattaggcca cggtacatga
     5281 gcagactgtt ttcaattgac acaagatcat catcacaaca atggagtaaa aacacaggag
     5341 gaaaacatga tgtgacatta agatccgcac tatattgtat tatattttct gaggtactat
     5401 tgtcccccag taattgcttc ctggagcctg gatgtgtcac atcatctttc attgatatta
     5461 caggataaat taacccgaca aaatcgggaa tagcgaaaat ctcttgttct ttactgtcag
     5521 aagaacgttt gaaatcacaa atctgattca caaacgttga ggcgatatgc cccccagcag
     5581 aaaatcctaa tacaccaata cgatgacgct gtagatgcca tttatcagca ttcttacgaa
     5641 tataaagcat tgcagatatt gcatcaataa aagttttgtt tatattttct tcctcagctg
     5701 gagatggtaa tctgtaacta agaacaaata atgtatagcc ctgagataaa aactctggag
     5761 caagagctgt accttcctta tctaacataa cagcctggta gccccccccg ggaataatca
     5821 atattcctgt tccatttggt ttatctggta aaaaaataat taactgagga gcataaatat
     5881 ctgtcaatac acgatcaagt tcaaat
//
LOCUS       DAZFKF010000199         5778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFKF010000199 DAZFKF010000000
VERSION     DAZFKF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.201"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..116)
                     /locus_tag="ACHKA1_005125"
     CDS             complement(<1..116)
                     /locus_tag="ACHKA1_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5682653.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRRAMDAPGRRVCA"
     gene            complement(127..345)
                     /locus_tag="ACHKA1_005126"
     CDS             complement(127..345)
                     /locus_tag="ACHKA1_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682654.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            complement(329..757)
                     /locus_tag="ACHKA1_005127"
     CDS             complement(329..757)
                     /locus_tag="ACHKA1_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682655.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAVTNEKQQNIQHSEEQQSV
                     VRRSLAAVPAASVLVPDDVADRVRRAVCEIRACEAGADPR"
     gene            complement(792..1292)
                     /locus_tag="ACHKA1_005128"
     CDS             complement(792..1292)
                     /locus_tag="ACHKA1_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5682656.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLKLLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            complement(1364..1789)
                     /locus_tag="ACHKA1_005129"
     CDS             complement(1364..1789)
                     /locus_tag="ACHKA1_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO5682657.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            complement(1859..2368)
                     /locus_tag="ACHKA1_005130"
     CDS             complement(1859..2368)
                     /locus_tag="ACHKA1_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO5682658.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSALLAATGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQNPVNRALKPGTVTAKIRAIWKVMHRQGFITDGAETALNRWVK
                     SQTAAQNGSEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            complement(2365..2661)
                     /locus_tag="ACHKA1_005131"
     CDS             complement(2365..2661)
                     /locus_tag="ACHKA1_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682659.1"
                     /translation="MDNSSLLFWCLYITSFFGAFVITRWLCRKIICFFDKRHPVERAA
                     DALIQQAIVLYSGEFFCRITTRDGWHIMIIPPTHHARWDEAEKAFHVRKKVNTV"
     gene            complement(2651..2848)
                     /locus_tag="ACHKA1_005132"
     CDS             complement(2651..2848)
                     /locus_tag="ACHKA1_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682660.1"
                     /translation="MNSLPAGWARPLMARKHHFFKTGENISICGRWLYLAHNREPDTF
                     ESPDDCAECRRRVNKEKDNGQ"
     gene            complement(2841..3173)
                     /locus_tag="ACHKA1_005133"
     CDS             complement(2841..3173)
                     /locus_tag="ACHKA1_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682661.1"
                     /translation="MSIELRSSYEYRKILIAGGMKPEDAEKIVSFMDKECDKRDVSEI
                     IMDDMILHSAVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIHMILS
                     SMEDKANE"
     gene            complement(3189..3539)
                     /locus_tag="ACHKA1_005134"
     CDS             complement(3189..3539)
                     /locus_tag="ACHKA1_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682662.1"
                     /translation="MRSYIDNEKLKTISDCLSLLAKIKETIEEIKFQLEYEPCGDDTW
                     RNSARKALAVFQKQRRTVEYRLAVLRQEEKERNIRRHELVNDFLVRELKERVPESVFF
                     ECEAVARSKALETD"
     gene            complement(3554..3865)
                     /locus_tag="ACHKA1_005135"
     CDS             complement(3554..3865)
                     /locus_tag="ACHKA1_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682663.1"
                     /translation="MITTLFVESDEPLVCAAGMSVCGGTLTGVYFGDLRGYPWHSLDD
                     AFPPDMEAVVLIVQYGDQQELRIGHMGYEGFFIDEETGACLEDEDGQVTHWCHISALP
                     E"
     gene            complement(3862..4413)
                     /locus_tag="ACHKA1_005136"
     CDS             complement(3862..4413)
                     /locus_tag="ACHKA1_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFO5682664.1"
                     /translation="MMILQPMGRKGRAPAHVRAWTPEEDALLIALYPSTPVKDIAVRI
                     KRSFWGVHHRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVRLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHRRTAEDVVLRELMP"
     gene            complement(4417..4932)
                     /locus_tag="ACHKA1_005137"
     CDS             complement(4417..4932)
                     /locus_tag="ACHKA1_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682665.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFCFKGLRFMDDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILHGPVKWFCVDDVIYLQCEDDSAKRKMTEILSD
                     EFYAAEKRVRG"
     gene            complement(4932..5465)
                     /locus_tag="ACHKA1_005138"
     CDS             complement(4932..5465)
                     /locus_tag="ACHKA1_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682666.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCSILAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITSRVLRY
                     LIVDRTAAPAGISAEVA"
     gene            complement(5469..>5778)
                     /locus_tag="ACHKA1_005139"
     CDS             complement(5469..>5778)
                     /locus_tag="ACHKA1_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682667.1"
                     /translation="GPCFIARPDESCPEGPAIAAMVEAAERELRKRPDFLVWLCEKLG
                     VMRIPSMFNTDSWWTPSLSRDALCVVFKVGAYGREINGCIPEECQEIKHSEYVALTEE
                     "
BASE COUNT         1403 a         1619 c         1336 g         1420 t
ORIGIN      
        1 gcgcagacac gtctgcccgg agcatccatt gcccggcgct tacgtgtcag ccggttacaa
       61 agggcgcgtt ctgatccccg ctccatcaca gcctgagcac gatctgaatc atccatacct
      121 ttttccttac ttcgtctttc ctgccggaac agcaggcgtt ccggccatat cagtaaattc
      181 atgttgtcgc cgcgcctcca gacgacgaat actggcctta tccacgttgc agttcttgat
      241 caccgccagc agttgcaggt tgtaagtgac cgatgcccca aacgtgaacg gctccggcgc
      301 aggagggacc aggcagtcag ccagccattc agcggggatc ggcaccggct tcacaggcac
      361 gtatttcaca gacggcccgg cgcacccggt cagccacatc atcaggaaca aggacagagg
      421 ccgcaggcac tgctgccagc gaccggcgga caacgctctg ctgctcctcg ctgtgctgaa
      481 tgttctgttg cttttcattc gtgaccgcct cactgatttg tgaaatgagt tgcagggtat
      541 gctgctgatt tgccagcacc tgacgcgcct catcccgctc acgcacaagc acctgattat
      601 tgtcttcaag gcgctgcgtc ttctggtgca ttccccatat cgcggcaacc agtacagcga
      661 taatccccag cgccgccatg cacgccccgg caatcagggc cggtaaggca ttattcaccg
      721 cagccacagg acaccccgta acagcagtac cgcacacagc agcgctatca tgagcggtcg
      781 ccagaagcgg ttcagacttt cggcaagtcg tagcgacata accattcatc cgccgaacgg
      841 cgggcctcaa gaccgggaag tttcaccccc tttgaataaa tccagcggat atactgctta
      901 caggacgcgg gcatctgtcc ggcattgatc aggcgcagaa gcgtggagtt gcgaaagttc
      961 gtttcaccgg cccagaagat ccacgaagcc agcgccaccg tctgaccacg ggtaagcggc
     1021 acctgaacac gccgatcaat ggccgcaaag gcccatttca tgtccttttc cagcagtttc
     1081 agacattctt catccgtcag ggtcataccc ggtttcacat ccgggccggt atggccgtaa
     1141 cagatggtgg gtgtaccggt gggatcgata tacgtggagt tttctttccc ttcccagtaa
     1201 ccggtgtaat gcgtggcaat cgtgaacgtc ccggcaccgg ccagaacaag ggcaatcagc
     1261 tttttacgca gtggtgcggg gagttttggc attgttacgc tgtctcatga tgttctctga
     1321 gtcagcgtaa cggtgaagga aaggcggcag gctttgcgga atatcagaaa agtggcggtt
     1381 gcgttcttgc cagatgcaga cggcgctgtt cacgtatgac ggtataaatc tgggtttctg
     1441 acatccggta atggcggcga agggattcaa ttttctcgcc ccggtcccag cgcgaaaata
     1501 tctcattgtt tcgcagctcg gcaaagagag attccccgac cggcaggtaa taaccccgtc
     1561 cgcccatata ggctgcctga gccgcagcca ccttgcgtgc cagtttaccg gccagtaccg
     1621 gctcaatccc ctgacgctga agttctgcgc cggtgacttc aaccagttct gacagggtcc
     1681 gcggccagtt ctttttgaga acatcatcag ggatgtcatc aaggcggtcg ataagggcgt
     1741 gcagttgctc actgtcacca ccaaacatgc tcatctgagt ttctgccata tcagcctccg
     1801 gttctcgcgt tcccggtaag tttaaaataa aaatcccgcg ttgtggcggg atttggagtt
     1861 aacggggaag tgatttttca tactgcctgc aaacggcgtc ataccccatc agcgtgcgtt
     1921 cgggcattcc catcgcggcc agcattttgc ggcggtgcca tcgcttgaga cgctccagca
     1981 catctgaggc cagggcgggg tgttgctcca gccactgcca gtttgccaca ccttcgctgc
     2041 cgttctgcgc ggccgtctgc gatttcaccc agcggttaag ggcggtttcc gcaccatcgg
     2101 tgataaagcc ctgccggtgc atcaccttcc agatggcgcg aattttggcg gtcaccgtac
     2161 ccggttttaa ggcccggtta accgggttct gacgcacttt aaaaccgcgt tttttgaaaa
     2221 catccagcac gcgggataac tcatccggtg acatatcccg gcagctggtt ttgccggtgg
     2281 ctgccagcag cgcggatgta taagtctcat catccagggc aagctcgcgc tttgccacat
     2341 gaatggcgga tatcagtttt cctctcatac cgtatttacc tttttacgga catggaaagc
     2401 cttttctgcc tcatcccacc gggcatggtg tgttggtgga ataatcatta tgtgccagcc
     2461 atctctggtt gttatccggc agaaaaattc accgctatat aacacaatgg cctgctgaat
     2521 gagcgcatca gccgcccgtt caaccggatg tcttttatca aaaaagcaga tgatttttcg
     2581 acataaccac cgggtaataa caaatgcgcc gaaaaaggat gtgatatata agcaccagaa
     2641 caacagagaa ctattgtcca ttatctttct ccttattcac tcttctgcgg cattcggcac
     2701 agtcatcagg gctttcaaat gtatccggct cgcgattatg agccagatat aaccaccgtc
     2761 cacatatact gatattttcg cctgttttaa aaaaatgatg cttcctggcc attagtggcc
     2821 gcgcccatcc agcgggtaga ctattcattt gctttatcct ccatactgga gagaatcata
     2881 tgtattaatg tcgggcgtgg agatatacgc atttcattaa gcgtctgaag ggcagcaaca
     2941 gctgcctgtt tatcggtgcc tttggcaatt tcagcaagag aatgcacaat ccataacggg
     3001 cttaacgcta cggctgaatg cagaatcatg tcatccataa taatttctga cacatcccgc
     3061 ttatcacatt ccttatccat aaaagaaacg attttttctg catcttccgg tttcatgcct
     3121 ccggcgatga gaattttacg atattcataa gacgatctta attcaatact catcattcac
     3181 ctgcctgttt aatcagtttc cagtgctttg ctacgggcaa cagcctcaca ctcaaaaaat
     3241 accgactccg gcacacgctc ctttaattca cgaacaagaa aatcattaac gagttcatga
     3301 cgtcttatat ttctttcctt ttcctcctgg cgcaatacag caaggcggta ttcaacagtc
     3361 cggcgttgtt tctgaaagac agccagtgct tttctggcag agttacgcca tgtatcatcg
     3421 ccacagggtt cgtattcaag ctgaaattta atctcttcaa tggtttcttt tatttttgcc
     3481 agcaagctaa gacagtcgct gattgtcttc agtttttcat tatcaatata acttctcacg
     3541 cattcacctc ccgtcattcc ggtaaagctg aaatatggca ccagtgcgtc acctgtccgt
     3601 cctcatcttc aaggcaggct ccggtttctt catcaataaa aaagccttca taccccatat
     3661 ggccgatgcg cagttcctgt tggtcaccat actgaacaat cagcacgaca gcctccatat
     3721 caggcggaaa tgcgtcatcc agtgaatgcc agggataacc gcgcaaatcc ccgaaataaa
     3781 caccggtcag cgttccgcca caaaccgaca ttccggcggc acataccaga ggctcgtctg
     3841 attcaacaaa taatgtcgtt atcatggcat taactcccgt aatacaacat cttcagcagt
     3901 ccggcgatgg tgataaataa aattcaccgt actctcggat aattcaaatt tctcgcctat
     3961 ttcccggaag gtaagacgtc gtggtgattc agcatcccgt aattcacgaa taagacgaac
     4021 atcatcatca ggtatacggg tgaagggcag caattctccg tattttttca tgcttacgcc
     4081 aatatatcgc gcccgattgt ggacagaatc ccggtctcgc cccagatatt ctcctatttg
     4141 cttaccggtc attgttctgg cattttttcg gataaattta tcttcatcat gtttaaatct
     4201 tggacgtttg catttgagca actccgggta agtaccgcgt aataaaacaa tccggtgatg
     4261 tacaccccag aaacttcttt ttatcctgac agcaatatcc ttaaccgggg tggatggata
     4321 aagcgcaatc agcagtgcat cttcttcagg tgtccacgca cggacatgag cgggtgcacg
     4381 gccttttcgc cccatgggtt gtaaaatcat catacatcac cccctgactc gcttttcagc
     4441 agcataaaac tcatcagaca gaatttcggt cattttccgt tttgcggaat catcttcgca
     4501 ctgaaggtaa ataacatcgt caacgcagaa ccacttcacg ggaccatgca atattgctga
     4561 aaagtcaatg cccagccaga acaacagggc atcggttctg gcatacgtga cgggcgaata
     4621 ttcacgccac agactgttaa gttcgtcaga ggcaacgcgc agggctttgg gaatacgtga
     4681 tgtgcgcggt gtgcagctcc agccattcga cgcagtcggt tttcgccata agtcgcgatg
     4741 aaaaggatat ttgtcatcca taaagcgcag ccctttaaaa caaaagccac tgataccgga
     4801 tacaaatacc gaccggcacg caacattcag cacggcctca aggcgttttg cctcatcctt
     4861 aactttctgg cagtcctgct gatatttttg ccacgcggcc agcgcggaag ggtttgatgt
     4921 tttaaagaac attacgccac ctccgcagat atcccggcag gcgcggccgt ccggtcaaca
     4981 atcaggtaac gcagaacgcg cgaggtgata ttccagccgc ttaaaccgca gataatgacg
     5041 cccagacgga tgtcgatata cgccataaca aactggggca caccttcttt catggcctgc
     5101 tcatcaactt cagccaccac ataaacggtt tcacaggtca gaacgccctg agcaaattcc
     5161 cacgccattg acggaatatc atgcccctca atccacggca gggattttct gaagctgcac
     5221 cactgaacat catcagccgc accttcctgg ctgcatttgc gacgaaccgg ctgcaccggg
     5281 cggggacgcg gaatatcata aaaaccgtta cattccgtca gcaccccggc atcgaccgca
     5341 tcacgcagaa aataaaccat cgaggatggc ggcatattca ttttttcagc cagaatgctg
     5401 caggtcagac gcccgtaaat actcagccag tttttaaccc cttcaagtac ttttgcatca
     5461 atcatgaatt attcctccgt cagcgccaca tattcagaat gtttaatttc ctggcattct
     5521 tcaggaatac acccgtttat ttccctgcca taagcgccta ctttaaacac cacgcacagg
     5581 gcatcacggg agagtgacgg ggtccaccag gagtccgtgt taaacatgga ggggattctc
     5641 attaccccca gcttttcaca gagccagaca aggaaatcag gacgctttct taactcgcgt
     5701 tcagcggctt caaccattgc ggcaatcgcc ggaccttcag ggcatgactc atcaggtctg
     5761 gcaataaagc acggtccg
//
LOCUS       DAZFKF010000200         5732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFKF010000200 DAZFKF010000000
VERSION     DAZFKF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.202"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            36..1385
                     /locus_tag="ACHKA1_005140"
     CDS             36..1385
                     /locus_tag="ACHKA1_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFO5682668.1"
                     /translation="MLVSKSNELNTSAFFASRNYNGNNSSNPMELLAHSIIKLICKEA
                     ASATYCGALETLQKMMSECIYQEGNAFVIMGAGEQLKRIKYDVDENNLKVFNVHFDNN
                     EVLVTDGEPDVVCLSKQVWENLLIKLKPEIKENAASEVHKSANKGEIEQLVECSKRNE
                     QTLFDNIRKSDFHVGSLKPGSMNNVILEMSPNVCMEPRNSYENKIDEVSSLSEPKEHT
                     IDIQEKTDAFVKKFKGVLFDKNTRSSELLFNFYECCYVFLPRAQPQDKIDSYNSALQA
                     FSIFCSSTLIHNNIGFDFKLFPEVKLSGEHLETVFKYKNGDDVREIAKINITLQKEEG
                     GLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGTSLFDVDTPCIFNTPANHESYEKSLKP
                     VSENGLNGVLSDRNKKIKMITGVAPFDDILFMDDDFDDNSPEDAPIENSPIVNSPLV"
     gene            1754..1912
                     /locus_tag="ACHKA1_005141"
                     /pseudo
     CDS             1754..1912
                     /locus_tag="ACHKA1_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            2136..2255
                     /locus_tag="ACHKA1_005142"
     CDS             2136..2255
                     /locus_tag="ACHKA1_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO5682669.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            2320..>5732
                     /gene="ypjA"
                     /locus_tag="ACHKA1_005143"
     CDS             2320..>5732
                     /gene="ypjA"
                     /locus_tag="ACHKA1_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFO5682670.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPGMAAFTSDVIGVVNDETV
                     DGTQRVDERGTTNNTHIINHGQQNVHGGVSNGSLIESGGYQDVGSHNNFVGQANNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYKGGISNGTTVKGGASRVEGGSANGTLIDGGSQ
                     IVKVQGHADGTTINKSGSQDVVQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIVDNTSTSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGK
                     LIVQKEAKTDGTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGSVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQVVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNT
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTKINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISGNLATNMLLENGGNLLV
                     LAGTEARDSTVGKGGAMQNLGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAQTAAPATTNGIYNTLTT
                     NELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDA"
BASE COUNT         1756 a         1126 c         1413 g         1437 t
ORIGIN      
        1 aaatagccat gtagacatta attgaggaat agacaatgtt agttagtaaa agcaacgaac
       61 ttaacactag cgcatttttt gctagcagga attataatgg aaacaattct tccaacccca
      121 tggagctact agctcatagc attataaaat taatttgtaa ggaagctgcc tcagcgacgt
      181 attgcggtgc tcttgaaact ttacaaaaaa tgatgtctga atgtatatat caagaaggca
      241 acgcctttgt cattatggga gctggagaac aattaaaacg tattaaatat gatgttgatg
      301 aaaataactt aaaggtattc aacgtacact ttgataataa tgaagtgtta gttactgatg
      361 gtgagcctga cgtagtatgt ttaagcaagc aggtctggga aaatcttctc attaaattaa
      421 aaccggagat caaggaaaat gcggcttctg aagttcataa atcagcgaat aaaggtgaga
      481 ttgagcaatt agttgaatgc tctaaaagaa atgaacagac ccttttcgat aatataagaa
      541 aaagtgattt tcatgttggt tcacttaagc caggtagtat gaataatgtt attttagaaa
      601 tgtcaccaaa tgtctgtatg gaaccacgta attcatatga aaacaaaata gatgaggttt
      661 catctttgtc agagccaaag gaacacacca tagatattca agaaaaaaca gatgcgtttg
      721 tgaagaagtt caagggggta ttatttgata aaaatacaag gtcttcagag cttctgttta
      781 atttttatga atgttgctat gtgtttttac caagagcgca gcctcaggat aaaatcgata
      841 gctataattc agcactgcaa gctttttcca tcttttgttc atctacgttg atacataata
      901 atataggctt tgatttcaaa ttatttccag aagtcaagct gtctggagaa catcttgaaa
      961 cggtattcaa atacaaaaat ggcgatgatg tccgggagat agccaaaatt aacattactc
     1021 tccaaaaaga agagggtggc ttatataatc tacgtggatt ggattttaag gggtgcttct
     1081 tttctggaca gaacttcagt aactatgata ttcaatatgt gaactgggga acgtcattgt
     1141 ttgatgttga tactccgtgt atttttaata cgcctgctaa ccatgagagt tatgaaaaat
     1201 cattaaaacc tgtaagcgaa aacggtttaa atggagtctt atctgatcgt aataaaaaaa
     1261 taaaaatgat cacgggtgtg gcaccattcg atgatatttt atttatggat gatgactttg
     1321 atgataactc ccctgaggat gctcccattg agaatagtcc tattgtgaat agtccccttg
     1381 tatgaagttc taaaagtagt tttcaatgat aaataaatgg ctatttttac gaagagataa
     1441 taacgtagat tgaaacaata tgtattattg attgagtaaa tgggattatt cttcacacta
     1501 catccatttt agtgtcacta ccaaagaaaa aggggctacg ttttcatgta accccttgat
     1561 ttatttggtg ggctagcagt gactgaattg gtgctgtaat tggttgaaat atttcatcta
     1621 atgtctggtt caacttcctt agctgcctga aaattttttg agcttaccca gcaataacag
     1681 cgttacggtg caccacgcct gactgaagaa ctgcgtgctc agggttacca cttcaacgta
     1741 aaaccgtggc aggaggtccg gactgaaaaa actgctcagg acatcatcgg cagggaacgc
     1801 actgctagtc tgtaaactga accgactggg gcgcagtatg tggcatcttg tcgtgctgtt
     1861 ggaggagctg tgcgaacgtg gtattaactt tcgtgctctg gcccaatcta tatttgccca
     1921 acaatggggg gacgaatgct gtaaaagtaa aacaatctgc gatctcaaag ttattgtgtg
     1981 atttttatgt gagcagaaga tattcatcag aaacgattat gtaaatcatt ttattttgcc
     2041 aacggcctca ttgtcgaaag aaaagaatac gcccgtgttt cctgaataga ttgacttttt
     2101 tatccaacca cacttcagcg cactgcgttt aaaaaatgcc tcattcttat gcggaatatc
     2161 atcatttcat catgatgtct ttgatgagcg gtgaacacaa tacacttgcg ctgtctcttc
     2221 aggatgaatc ccctggtctg gtgcctgtgg gctgatgttg cagcaaagct aaggtcgctt
     2281 aaacgctact cagtattcac ttttcagagg atgaaattta tgaacaggac cagtccccat
     2341 tattgtcgcc gctcagtact ttccttattg atatctgcct tgatatatgc cccgcccggg
     2401 atggcggcct tcacttctga tgttattggt gtggtaaacg atgagactgt agatggcacc
     2461 caaagagtag atgaacgagg tacaacaaat aacactcata ttatcaacca tggccagcag
     2521 aatgttcatg gcggggtatc taatggaagt cttattgaat ctggtggata tcaagatgta
     2581 ggaagtcata acaattttgt ggggcaggct aataatacaa ccattaacgg gggcagacag
     2641 tcaattcatg acgggggtat ttccacaggt acgataatcg agagtggcaa tcaggacgtt
     2701 tataaagggg gtatcagcaa tggaacgaca gttaagggcg gtgcttcacg cgtagaggga
     2761 gggagtgcga atggaacact cattgatggt ggtagccaga tagtaaaagt tcaagggcat
     2821 gctgatggta caacgataaa taagtctggc tctcaggacg tagtacaagg aagtctggca
     2881 acgaacacaa ccataaatgg tggtcgacag tatgttgaac agagcacagt agaaacaacc
     2941 accatcaaaa atggcggtga gcaaagagta tatgagagcc gtgcgctgga cacgacgatt
     3001 gaaggcggaa ctcagtctct gaatagtaag tcaacggcaa aaaatacgca gatctattct
     3061 ggtggcacgc aaattgttga taacaccagc acctcggatg ttattgaagt ttattccggt
     3121 ggcgtgcttg atgttagtgg tggtacggca acaaatgtta cccagcacga tggtgcaatt
     3181 ttaaaaacta acactaacgg tacgacggtg agcggtacga atagtgaagg tgcattctcc
     3241 atccacaatc acgtggcaga caatgtgttg ctggaaaacg gtggtcattt agacataaac
     3301 gcatatggtt cggcaaacaa gacgattatt aaagataaag gaacaatgtc agttttaacc
     3361 aatgctaaag ctgatgcgac ccgaatagat aatggcgggg ttatggatgt tgcaggaaac
     3421 gcgacaaata ccataattaa tggtggcaca cagaatatta ataattatgg catagccaca
     3481 ggcaccaata tcaacagcgg aacgcaaaat atcaaaagcg gcgggaaagc tgacacaaca
     3541 attatatcct ccgggagccg gcaggttgtt gagaaagatg gtacggcaat tggcagcaat
     3601 attagcgccg gaggctcgct gattgtctat accggcggta ttgcacatgg ggttaaccag
     3661 gagacgggca gtgctttagt tgccaacacg ggtgcaggga ctgatatcga aggatacaac
     3721 aagctctctc acttcactat taccggaggg gaggctaatt atgttgtgct ggaaaatacc
     3781 ggcgaactga cggtagtggc taaaacctcg gcgaaaaata ctaccattga tgctggcggt
     3841 aagctgattg tccagaagga ggctaaaaca gatggcacca gacttaataa tggcggcgtt
     3901 ctggaggttc aggacggtgg tgaggctaag catgttgagc aacaatccgg cggcgcatta
     3961 attgcttcca cgacctccgg aacacttatc gaaggaacca acagttatgg tgatgctttc
     4021 tacatcagga attcagaagc taaaaatgta gtgctggaaa acgctggctc attaacagtc
     4081 gtcactggtt cccgggcagt tgacacgatt attaatgcca acggcaaaat ggatgtttat
     4141 ggaaaagatg ttggctctgt actcaatagt gctggcaccc aaacaatata tgccagtgcc
     4201 acttctgata aagcaaatat caaaggtggc aagcaaacgg tatatggttt agccactgaa
     4261 gcaaatatcg aaagtggtga acaaattgtt gatggtgggt caacagagaa aacacacatc
     4321 aatggtggca cgcaaaccgt tcagaattat ggtaaggcga tcaataccga tatcgtctct
     4381 ggcctacaac aaattatggc aaacgggaca gcggaaggtt ccattattaa tggcggttca
     4441 caggtagtta atgagggcgg tctggctgaa aactcggtgc ttaatgacgg cggcacactc
     4501 gatgtgcggg agaaaggcag cgcaacgggg atacagcaga gtagccaggg cgcgttggtt
     4561 gcaaccacca gggcgacgcg ggtcacagga acacgcgcgg atggcgtcgc gttcagcatc
     4621 gagcagggtg cggcgaacaa tatcctgctg gcaaatggcg gcgtgttaac cgtggagtca
     4681 gacacctctt ctgacaaaac acaggtcaat acgggcggac gggagatcgt caaaacaaaa
     4741 gccactgcga caggcacgac gctcaccggc ggtgaacaaa ttgtcgaggg tgtggcgaat
     4801 gagacaacaa ttaacgacgg cggaatacaa acagtttcag ctaacggaga ggcaataaaa
     4861 acaaagatca atgaaggcgg tacgctgaca gtcaacgata atggcaaagc gacagatatc
     4921 gtccagaaca gcggtgccgc tctccagacg agcacggcta acggtattga aatcagcggt
     4981 actcaccagt acggcacttt ttccatttcc ggcaatttag cgaccaatat gttgctggaa
     5041 aatggcggta atttattggt attagcaggt accgaagctc gcgactccac ggttggcaag
     5101 ggtggggcaa tgcaaaacct gggtcaggac tccgccacaa aggttaactc tggcgggcaa
     5161 tatacccttg ggcggtcaaa agatgagttt caggctctgg cccgggcaga agatctccag
     5221 gtcgctggcg ggacggcaat cgtctacgca ggtacgctgg cggatgcatc ggtcagtggc
     5281 gcgacaggaa gcctgtcgtt aatgacgcca cgggataatg ttacgccagt taaactcgaa
     5341 ggggcggtcc ggattaccga tagcgcgaca ttaactctgg gaaatggcgt cgatacaacg
     5401 cttgccgacc tgacggctgc cagccggggc agtgtctggc ttaacagcaa taattcctgt
     5461 gcaggtacca gcaactgcga atatagagta aacagtttgc tactcaacga cggtgatgtt
     5521 tatttatcag cacaaacagc agcgcctgcc acaactaacg gtatctacaa tacgctgaca
     5581 accaatgaac tttccggtag cggtaatttc tacctgcata ccaacgttgc aggctcccgg
     5641 ggcgatcaac tggtcgtcaa caacaacgcc actggtaatt ttaaaatctt tgttcaggat
     5701 accggcgtca gcccacagtc tgacgacgcg at
//
LOCUS       DAZFKF010000201         5724 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFKF010000201 DAZFKF010000000
VERSION     DAZFKF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.203"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..612
                     /locus_tag="ACHKA1_005144"
     CDS             <1..612
                     /locus_tag="ACHKA1_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO5682671.1"
                     /translation="PGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGNEQLYMHAQKN
                     MDTEVLNNRTTDVKVNHTETVGGNQAISVTGVQSTSVKSAQFTHVESLRQVAVQQGQL
                     IVTGGDHEILVQETLHLAAGKAIHLQCGESELVMLANGTVALSCTNFNLYAAQRGQIT
                     AAQILDLNMDGAKSGIEQPGPLTPSAISERVTAEFPPQQPENK"
     gene            612..1040
                     /locus_tag="ACHKA1_005145"
     CDS             612..1040
                     /locus_tag="ACHKA1_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO5682672.1"
                     /translation="MPDYHFNEGQITLPASGRDCTTHVLRLPELQAVLTISRDILSGT
                     MTLEEYVADQIAKIKRDARHVQIPEPQFTDMKNGKHVCQFSCQAEISGLVHYQQQHII
                     LSGQSLLAFAWTLGHPFTEQDHQTWRNILASFVPRETVTE"
     gene            1045..5685
                     /locus_tag="ACHKA1_005146"
     CDS             1045..5685
                     /locus_tag="ACHKA1_005146"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5682673.1"
                     /translation="MGTDYLAAKMEDPLEHTSVLGDFMGGLVEGVCYGVLFCASANPV
                     GLAVGLTAGFIFSDVISEIGDTVSSWFPPDVAGTITSGSKNVNVKSKPAARAAGAVPD
                     EILLALLEAEAANPPDSKGMQLVKGIVAKSIMIAKLPLLPAELGRMAGEKLAAYLKGE
                     STPSQEDEQGFWSRVWESISNPVVDGPDPALHVETRLLDTIDCHKHRPQPIQYLAEGS
                     ETVRINSQPACRNGDRSTCEAVIAHTQASAKVRIGGRSLVVKEIHTGKNMLAYYAGLI
                     AGGFLVSSVQALFCRTTAREFAGKLPCLMLGSVLGNTLGIVAGGTLSTSLSSLSSGLQ
                     ASSQTARPVHIASGVKILGGEDELDFACDGLIPLAWQRIYSSLNDNEGILGRGWCLPF
                     EVFLHQEPAVPGAQDSAQIFFHDMSGRELGLGDVPVGGAMFYVDEGFRLYHPLAGMFV
                     LESAQGQYQIFEADPLHDGRFRLIQECDRHLNGIRYYYNDRGLPVRIEDDLQAICLHL
                     DYHPQHSRLTAVYQQYRQEDTLPGVLRIRYEYNEQVQLTAVVDADGVVMRRFAWDEEH
                     GRLAMHQTADGLQAHYRWQPTSGLPGHFHVARHWLQEGERRSGEILFDYDPAARRATL
                     HYGDGRPDSLHEWDEQYNITRWQPAPDICWLYEWGDNRELLRVVDPAQCEYGFKYDER
                     GNLIQVVDPVGQEYRFDWDRDFAFPLKEEYPEGLVLRHVYNVSGDRIKTIDQAGLITR
                     YNHDASGLVESVQDARGNLYRYAWNNRGQLTRQTDCSGYTTHLYYDEYGRLAAVTDAQ
                     GNCTRQTFSAAGRLQKQIRPDGGETTCHYNARGQLAGVTDPARQSSRFDYNLRGQVIR
                     HTDPQQRQVRFEYDAGGQLIALYNQKNEAYRFSRDSLSRLEESCDLTGCREVYRYDSR
                     SHVSEITRYPAPAVSGQETFLPQVTRLAYDAAGRVQYRENGDGAVCYRYGKNTLSLER
                     FAPDDWQKKQRGYENIAAQDTLHLVTDAYGSLLEEHNPAGIFTHRYDELGNRTETQMP
                     DGRRLKRLYYGSGHLQQLNLEDNDGQQTVLAEFERNSLHQEISRTQGSLTLRTEYDAA
                     GRITARLAQMAQQRHGTPLLERRYSWDQRGFLMSRYHRTQDAQALLSPEERSGSTWYQ
                     YDCAGQILSAQQHFTVRQYCDLAGNLMEERSPAIKNNQLMQYRGWHYQYDAFGRMSRR
                     TDEQAQRQDYRYNSDNRMVEVRFADRTHSRWQRVEYDYDLLGRRTGKRLYRWHDEGEA
                     EPAPERVRFIWDGMRLRGEDSPGKATEVLYVYNGDSYEPLARVDRFTRQVIEPQDTRP
                     PKTTVYYYHTALNGLPEAMTSESGQLVYEARYELWGKETEAASAHTVLTVGQNLRFAG
                     QYFDAETGLHYNTFRFYAPECGRFTQPDPIGLRGGLNLYSYVPNPLSWIDPLGLKCWS
                     TEQKNYWKDVGAKELTNPSGLYSPRNILEMIAGRAPKFTARVKVIKTGKIETRDIPYE
                     LHHKNIPQRQGGPGVHNKSNLDEVDPWQHQDTDKYRHSGIELIEVITGVNSW"
BASE COUNT         1354 a         1444 c         1731 g         1195 t
ORIGIN      
        1 cccggcagcc tgccggggac aaagacgcag atgaccatcc gttcgaagac gtatatgggc
       61 agcgggttta atgagctgaa gttcgatgat gcaacgggta atgagcagtt gtacatgcat
      121 gcccagaaga acatggatac cgaggtgctg aataaccgga ccacggatgt gaaggtaaac
      181 cataccgaaa cggtgggagg caaccaggct atctcagtga ctggggtgca gagtaccagc
      241 gttaaatctg ctcagttcac gcatgtggaa agtttacgtc aggttgcggt gcagcagggg
      301 cagttgatag tgaccggcgg ggatcacgaa attctcgtac aggagacgct gcatcttgcc
      361 gcaggaaaag ccattcatct gcaatgcggt gaatctgagc tggtcatgct ggccaacggt
      421 acggttgccc tgtcctgtac caatttcaat ctttatgccg cccagagagg acaaattacc
      481 gcagcccaga tactggatct gaatatggac ggcgcaaagt caggtatcga acagcccggt
      541 ccactgacgc cgtcggctat ttctgagcgt gtcaccgccg agtttccccc gcagcaaccg
      601 gagaataaat aatgccggat tatcacttta atgaaggaca gattacgtta ccggcttcag
      661 ggcgggactg taccacccat gttttgcgac tgccggaatt acaggctgtg ctgaccatct
      721 cgcgggacat tctgtcagga accatgacgc tggaagagta cgtcgccgat cagattgcaa
      781 aaattaagcg cgatgcacgc catgtgcaaa tcccggagcc gcagtttact gatatgaaga
      841 atggaaaaca cgtctgccag ttttcctgcc aggcagagat ttccgggctg gttcattacc
      901 agcaacaaca cattatcctg tccggacaat cgctgctggc cttcgcatgg acgctcggtc
      961 atccgtttac tgagcaggac caccagacct ggcggaatat cctggccagt tttgtcccgc
     1021 gtgagacggt aacggagtaa tgtcatggga acagattatc tggcggccaa aatggaagat
     1081 cctcttgagc acacttctgt cctgggggat ttcatggggg gcctggtcga aggggtatgc
     1141 tatggggtac ttttttgtgc ctctgccaac cctgttggac tggcggtagg gctgactgcc
     1201 ggctttattt tcagcgatgt tatttctgaa atcggcgata cggtcagtag ctggtttccc
     1261 ccggatgttg caggtacgat aacgtcaggc tcaaaaaacg ttaatgtgaa atccaagcca
     1321 gccgcccgtg cggcaggtgc agtgccggat gaaatattac tggcactcct ggaggcggaa
     1381 gcggccaatc caccggacag taaggggatg caactcgtta aagggattgt ggcaaaaagt
     1441 attatgattg ccaaactacc tcttttgccg gcagaactgg gcaggatggc aggcgagaaa
     1501 cttgccgctt acctcaaagg ggaaagtact ccgtctcagg aagatgagca gggattctgg
     1561 agtcgggtct gggaatccat tagcaacccg gtggtggacg gccccgatcc ggctttgcat
     1621 gtcgagacac ggctgctgga cacgattgac tgtcataaac atcgcccgca accgatacag
     1681 tatctggcgg aaggttcaga aaccgtacgt attaacagcc agcccgcctg ccgtaacggt
     1741 gatcgcagta cctgcgaggc ggtgattgcc catacccagg ccagtgcgaa agtccgtatt
     1801 ggcgggcgca gcctggtggt gaaggaaata cacaccggca aaaatatgct cgcttactat
     1861 gcagggctta ttgccggagg atttctggtc agctcagtgc aggctctgtt ttgcaggaca
     1921 acggccaggg agtttgcggg gaagttgccc tgcctgatgc tgggttcggt cctgggcaat
     1981 acgctgggga tagtggccgg aggaacactt tccacttcat tatcgtccct gtcttcaggg
     2041 ctgcaagcgt catcacagac cgccaggccg gtacacatcg cctccggtgt aaaaattctg
     2101 ggcggagagg atgagctgga ttttgcctgt gacgggttaa tcccgctggc atggcagcgg
     2161 atttacagca gccttaatga caatgaaggc attcttggcc ggggctggtg cctgccgttt
     2221 gaagtgttcc tgcatcagga gcctgctgta ccgggagcgc aggacagcgc gcagatattt
     2281 tttcatgaca tgtccggcag ggagctgggg ctgggagatg ttccggtcgg cggagccatg
     2341 ttctatgtgg acgaaggttt ccggctttat catccactgg ctggtatgtt cgtgctggaa
     2401 agcgcacagg ggcagtacca gatttttgag gcagatcccc tgcacgatgg tcggttccgc
     2461 ctgattcagg aatgcgatcg ccatctgaac ggtatccgtt attactacaa tgaccgggga
     2521 ctgccggtgc ggatcgagga tgatcttcag gccatctgcc tgcatctgga ctatcacccg
     2581 cagcattccc gtctgacggc cgtataccag cagtaccggc aggaggatac cctgccgggc
     2641 gtactgcgta ttcgttatga atataatgaa caggtgcagc tcaccgccgt cgtggatgct
     2701 gacggggtcg tgatgcgccg ttttgcctgg gatgaagagc acgggcgcct ggccatgcac
     2761 cagacggcgg atggcctgca ggcgcattac cgctggcagc ccacttccgg tcttcccggc
     2821 cattttcatg ttgcccggca ctggctacag gaaggcgagc ggcgcagcgg ggaaatcctg
     2881 tttgattacg atccggcggc gcgccgggcc acgctgcatt atggtgatgg aaggccggac
     2941 agcctgcatg aatgggatga gcagtacaac attacccgct ggcagcctgc cccggatatc
     3001 tgctggctgt atgaatgggg ggataaccgg gaactcctgc gggtggttga tcccgcacag
     3061 tgtgagtacg gcttcaagta cgatgagcgc ggcaacctga tccaggtggt tgatcctgtc
     3121 gggcaggaat accgatttga ctgggaccgg gattttgcct ttccgctgaa agaggaatat
     3181 cccgaagggc tggtcttacg ccatgtttac aatgtctctg gggatcgcat caaaaccatt
     3241 gaccaggcag gcctcatcac ccgctataac catgatgctt ccgggctggt tgagagcgta
     3301 caggatgcac ggggcaacct gtaccgctac gcctggaata atcgtgggca gttaacccgg
     3361 cagacggact gttccgggta taccacccac ctgtattatg atgagtatgg acggctggct
     3421 gccgtgacgg atgcacaggg taactgcacc cgccagacgt tttcagctgc gggccgcctg
     3481 caaaagcaaa tccgccctga tggcggggaa accacctgcc attacaatgc ccgtggacag
     3541 cttgccggtg tgaccgatcc ggcacgtcag agcagccggt ttgactataa cctgcgcggg
     3601 caggttatcc gccacactga tcctcagcag cgccaggtgc gcttcgaata tgatgccggc
     3661 gggcagctta ttgcgttgta taaccagaaa aatgaagcgt accgtttcag ccgggacagc
     3721 ctgtcacggc tggaagagtc ctgcgatctg acggggtgcc gcgaggtcta ccggtatgac
     3781 agccgcagtc atgtcagcga aataacccgt tatcccgccc ccgccgtatc cggtcaggag
     3841 acgtttttac cgcaggtcac ccggctggcg tatgatgccg ccggacgggt gcagtaccgg
     3901 gagaacggag atggcgctgt ctgttaccgg tatggaaaaa acacgctgtc acttgagcgg
     3961 tttgcgccgg atgactggca gaagaaacag cgcgggtatg aaaatatcgc tgcgcaggac
     4021 acgctgcatc tggtcacgga tgcgtatggc agcctgctgg aagaacataa cccggcgggc
     4081 atctttaccc accgctatga cgaactgggc aaccgtacag aaacgcagat gccggatggt
     4141 cgccgcctga agcggctgta ctacggcagc ggtcacctgc aacagctcaa cctggaagac
     4201 aacgacgggc agcagacggt actggctgag tttgaacgta acagcctgca ccaggaaatc
     4261 agccggaccc agggcagcct gacactgcgc acggaatatg atgccgccgg gcggattact
     4321 gcccgtctgg cgcagatggc gcagcagcga catggaacac cgttgctgga gcggcgttac
     4381 agctgggatc agcggggctt cctgatgagc cgttaccacc ggacacagga tgcgcaggca
     4441 ctgctttccc cggaggaacg cagtggcagc acctggtacc agtatgactg cgccgggcaa
     4501 atactgagcg cacagcagca cttcacggtg cggcagtact gcgatctggc cggaaacctg
     4561 atggaggagc gcagtccggc gatcaagaat aaccagttaa tgcagtaccg gggctggcac
     4621 taccagtatg atgccttcgg gcggatgagc aggcgcacgg atgaacaggc gcaacggcag
     4681 gattaccgct ataacagcga taaccggatg gtggaagtgc ggtttgcgga caggacgcac
     4741 tcccggtggc agcgggtgga atacgactac gatctgctcg ggcgacggac ggggaagcgg
     4801 ctgtaccggt ggcatgatga gggcgaagcg gaaccggctc cggaaagggt gcgttttatc
     4861 tgggacggga tgcggctgcg gggtgaagac agcccgggta aggccacaga agtcctgtat
     4921 gtctataacg gcgacagtta tgagccgctg gccagggtgg atcgttttac ccggcaggtg
     4981 atagaaccgc aggacacgcg cccgccgaaa accacggtat attactacca cacggcgctg
     5041 aacggcctgc cggaagcgat gaccagtgaa tcagggcaac tggtctatga agcgcgttat
     5101 gaactgtggg ggaaagaaac ggaggctgca agcgcacata cggtcctgac agtggggcaa
     5161 aacctgcggt tcgcggggca gtattttgac gcggaaacgg ggcttcacta taatacgttc
     5221 agattttatg cgccggaatg tgggcggttt acccagcctg acccgatagg gctaagaggc
     5281 gggctgaacc tctatagtta tgtaccgaat ccgctgtcat ggatcgatcc acttggtctt
     5341 aagtgttggt caacagaaca aaaaaattat tggaaagatg ttggtgcaaa agaattaacc
     5401 aatccatcag gattatattc accaagaaat attttagaaa tgatcgctgg gcgtgcacct
     5461 aaattcacag ctagagttaa agtaattaaa acgggtaaaa tagagacaag agatatacca
     5521 tatgagttac accataaaaa cattcctcaa aggcaaggtg ggcctggtgt tcataataag
     5581 agtaatttgg atgaagtcga tccttggcaa catcaggata cggataaata cagacactca
     5641 ggaattgaac ttattgaggt tataacagga gttaactcat ggtaattgat aataactata
     5701 ttttattcgg tgcggcattg tccg
//
LOCUS       DAZFKF010000202         5680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFKF010000202 DAZFKF010000000
VERSION     DAZFKF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.204"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..531
                     /locus_tag="ACHKA1_005147"
     CDS             <1..531
                     /locus_tag="ACHKA1_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682674.1"
                     /translation="ENAYFICTPFFLLEERLKNSNLKQFNQGFDIIIEDTTFQMYSPN
                     RSGQINHVKRLLNNDGIFIFTEKHSSEHDEYQKREIQKDYSFKQRYFSKDEINTKKEI
                     VLNTMNLNEVSVDDMLSAIKQHFKYASIYWNSGNFYSIAASNSLKHISTFINLLPKAA
                     IPMEYVYEKLPRNISW"
     gene            538..1110
                     /locus_tag="ACHKA1_005148"
     CDS             538..1110
                     /locus_tag="ACHKA1_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HFO5682675.1"
                     /translation="MEYSTLLSFAIVTLSQTISIGPGVALVINNAFLHGLKSSIKTSI
                     YIRIGETIVMAISLFALSSTSSTEQHFHIIKIFGGGYLIYIGLMGLINKKNKQRKEQK
                     PFLIPLLNPKAYLFFAALIPTFIDNNTNITLNFFILGVLFIFISFLTDLIYIAISLTI
                     RDKLTPSFSRYISICSSIFILGTGIYFIFT"
     gene            complement(1168..1884)
                     /locus_tag="ACHKA1_005149"
     CDS             complement(1168..1884)
                     /locus_tag="ACHKA1_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682676.1"
                     /translation="MQLDEHSRKIAALVAYNDKQIKEMNRLITLLNERVNDLNTFTST
                     LEREGKVVSGISYSFRDSVSNVKFYINKGMDEASCKLQKSVSDTLTSLLREKLSFTLN
                     DVKKEWVDFEEQTSKEIKESLKQTTREAEVATGIIRKTMEKYTVTRIVEKVIVLIVLF
                     LLLMIAPVFYAEYLKMNISSLMEEQKKHESMLKEWKEKAPLAEFSKCGDYRKACVLVD
                     DESGYYFNEQSPNKKYAILK"
     gene            complement(1905..2990)
                     /locus_tag="ACHKA1_005150"
     CDS             complement(1905..2990)
                     /locus_tag="ACHKA1_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFO5682677.1"
                     /translation="MASYHCTVKTGAKGVALKHADYISRSGEYKNYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRAYSKSPERGGAKK
                     ARFGETPQERKSALVALRSHWADVQNEHLARHGHQSRVDHRSLKEQGIKRTPEVHLGP
                     VRVAVMNADQVCAILSRRNAEREKLEAEQERDSIIDLKTTLKEALAERDNIPPSLDEL
                     GAELKAFVESSFDELNEVMQLNSLINGALDSMMKQFQVEEERKLQQERLIEAEKQRDL
                     ERERERVRQRAYSSPAPSRSRDYGMSR"
     gene            3161..3607
                     /locus_tag="ACHKA1_005151"
     CDS             3161..3607
                     /locus_tag="ACHKA1_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFO5682678.1"
                     /translation="MTNLEEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAILAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFNAAELVGALAGVAELPRNHPKWQEWEKRGKELLTKDGA"
     gene            complement(3812..4717)
                     /locus_tag="ACHKA1_005152"
     CDS             complement(3812..4717)
                     /locus_tag="ACHKA1_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO5682679.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFTYGVRILPKDIAILARFIQQNQ
                     PHAQHWLVFDVDRTGAAIDWSDRNCPAPNITVKNPRNGHAHLLYALTLPVRTAPDASA
                     SALKYAAAIERALCEKLGADVNYSGLICKNPFHQEWQVVEWRLELYTLDELADYLDLS
                     ASARRRVDKDYGMGRNWYLFEKSRKWSYRAIRQGWPAFSQWLDAVIQRVEMYNASLLV
                     PLSPAECRAIGKSIAKYTYRKFSPEGFSAVQAARGRKGGKIGGAKSKRVAVPTSARSL
                     KPWEALGISRATYYRKLKCDPDLAK"
     gene            5270..>5680
                     /locus_tag="ACHKA1_005153"
     CDS             5270..>5680
                     /locus_tag="ACHKA1_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682680.1"
                     /translation="MNNFINECISAPRINGMAEFFSSTASGEKHKPFRLVEIDDRLFH
                     SYDYIKFAKHLTKNSGPFVNHYFCSIPYSKEEECRLGTTVIKYAITLNKKLNTWCLGM
                     AEGAMARTISELSKGNIQTLTTSPTKENEHAFFKY"
BASE COUNT         1717 a         1294 c         1090 g         1579 t
ORIGIN      
        1 gaaaacgctt attttatttg cactccattt ttcctgttag aggaaagact aaaaaattca
       61 aatcttaaac aatttaatca aggattcgat attataatag aagacaccac atttcaaatg
      121 tactcaccta acagatctgg gcaaataaac cacgtaaaaa ggctattaaa taatgatggt
      181 attttcatat ttacagaaaa acatagttca gaacatgatg aatatcaaaa aagagaaatc
      241 caaaaagatt attcattcaa acaaaggtat ttctcgaaag atgagattaa caccaaaaag
      301 gaaatcgtac taaacacaat gaatttgaat gaagttagtg ttgacgatat gctttccgca
      361 attaaacaac attttaaata cgcatcaata tattggaata gcggaaattt ttactcaatc
      421 gccgccagca attcactaaa acacatatca acatttataa atttattacc gaaagctgca
      481 attcctatgg aatatgtata tgaaaagctc ccacgaaata tatcatggta agtaaacatg
      541 gaatattcaa cattattatc atttgctatc gtaaccttat cgcaaactat atcaataggg
      601 cctggcgttg cattggttat aaacaatgcc tttttacatg gattaaagtc atcaataaaa
      661 acaagtatat acataagaat tggcgagact atcgtcatgg caatatctct tttcgcgtta
      721 tcctcaacat ccagcacaga acaacatttt cacattataa aaatattcgg tggaggctat
      781 ttaatataca taggattaat gggtttaatt aataaaaaaa acaaacaaag aaaagagcaa
      841 aaaccttttt tgataccatt attaaacccc aaggcgtatc ttttctttgc tgcgttaata
      901 ccaaccttta tagataataa tacaaatatc acattaaact tttttatatt gggtgtttta
      961 tttatattta tatcattcct aacagatctt atttacatag ctattagctt aacgataagg
     1021 gataaattga cgccatcatt ctcaagatac ataagcatat gtagttccat ttttatatta
     1081 ggtacaggta tttattttat ttttacgtga aacgccgttt ttatactcat gcagccttag
     1141 tcataagacg gcatgagtta cttaacacta tttcagtatg gcatattttt tatttggtga
     1201 ttgttcatta aaataatatc cgctttcgtc atcaactaac acgcatgctt tacgataatc
     1261 tccacacttc gagaactcgg ccaatggagc tttttctttc cattctttta acatggattc
     1321 atgttttttc tgttcttcca ttagcgaact aatattcatt ttgagatatt cagcatagaa
     1381 aacaggagcg atcattagca ataaaaataa tacaatcaac acaattacct tttcgacaat
     1441 ccttgttact gtatacttct ccattgtttt tctaattata cctgtcgcaa cttcggcctc
     1501 tctggtcgtc tgctttaatg attcctttat ctctttactt gtttgctcct cgaaatcaac
     1561 ccactctttc tttacatcat tgagagtgaa agataatttt tcgcggagta atgacgttag
     1621 tgtatctgag actgatttct gtaatttaca agatgcctca tccattcctt tattaatata
     1681 aaatttaaca ttagacacac tatccctaaa ggaataagaa attccagaaa caactttacc
     1741 ttcacgttca agagtacttg taaaggtatt taagtcattc actctttcat taagcagagt
     1801 aattagcctg ttcatttctt ttatttgttt atcattatac gcaacaagag cagcaatttt
     1861 tcttgaatgt tcatccaatt gcataaaata aaaacctcca gtgcttaacg gctcatacca
     1921 taatcacgtg aacgtgatgg tgctggtgag gaatacgcac gctggcgcac tctttctctt
     1981 tccctctcca ggtctctttg cttttcggct tcaattagtc gttcctgttg caacttccgc
     2041 tcctcttcca cctggaattg cttcatcatt gagtccagcg caccgttaat caatgaatta
     2101 agctgcatta cctcattgag ttcatcaaac gagctttcaa caaacgcctt caattctgcc
     2161 ccaagctcat ccaggcttgg tggtatgtta tcccgttcag ctaacgcttc cttcaaggtc
     2221 gttttaaggt caatgatgct gtcccgttcc tgctctgctt ctagcttctc ccgttccgcg
     2281 tttctccgtg acagaattgc acaaacctga tcagcattca taacagcaac acgaacggga
     2341 ccaagatgaa cttccggtgt gcgcttgata ccctgttctt tcagtgaacg atgatcaacg
     2401 cgcgactgat ggccgtgccg cgcaaggtgt tcattctgca catcagccca gtgagaacgt
     2461 agcgcaacca aggctgattt tcgctcttgt ggtgtctcac caaagcgtgc cttttttgca
     2521 cctccccgct ccggtgattt gctgtaggca cgtttaaaaa actgctgcgg atcgcgttca
     2581 ataccatcat taatccgctc gcaaaacatg atatgtgcgt gtggttgttc ctttccgtcg
     2641 attgcacctg gcggattgtg gatcgcgtac tgataggcat ggcgatcacc aagctccttt
     2701 tgcacgaaat cttcaacaag ttctatccgt tgttcgcgtg tcagttctcg cggtaatgcg
     2761 atctcaattt cacggtatgc cgtcccgttt tttcgttcaa attcatctgc tgctttccat
     2821 agctctgcgg gatttttctt cgcccatgat ggcatgttac cggatgacga aaattccaga
     2881 tcctcccgcg atttgtagtt cttatattcg ccggatctag aaatataatc agcatgtttt
     2941 aaggctacgc cttttgctcc agttttaaca gtacaatggt agcttgccat ctgccgttaa
     3001 gccctccgca ggagcgcact gtttctcgac gaaggagaga aacgtctaag tgcgcatccc
     3061 tgagtttatc agggatgatt ttacggtcac gtactcgggc atacaaggtg atattatgcg
     3121 caataccaaa acaataaacg gcaaaagtgc ggagataaga atgacaaatc ttgaagagtg
     3181 gttaagtgca aaaattgcct acatcaacgg tctgaaatca ccatctgagc agcagcgatt
     3241 acttgtatta ctggcagaga aaaaaaaccg aacaacgacg gatgaaaaaa cgttatccgc
     3301 actaatacga gcagaaaaaa ccgcggagaa agccgccgct gcaaaagcta gggtaacagc
     3361 aatccttgcg gcagaacgga aggctgccgc aagggcagaa cgaaaggcgc gggatcacga
     3421 actctacaaa gccgcaggcc tcatgattgt tgctggacta gtggacagca aaaccggaaa
     3481 accaaaattt aatgctgccg aactggtagg cgcactagca ggagtagcag agctaccacg
     3541 caaccatcca aaatggcagg aatgggaaaa acgcgggaaa gagctactca ccaaagacgg
     3601 tgcgtaagag tctccgttcg cgttctggta ccctggtgct tcctcgctca ttcgctcgct
     3661 acgctcggtc atgagactgc ggcgagcact accacctcaa tcatgcggaa agccgcacca
     3721 ttgcgggggt gagcagagaa atacacagca aaaccaacag ctcaaaaaaa cgccaacaga
     3781 gcgctgttat ctgataaggc tttatctggt ctcattttgc gagatctgga tcacatttta
     3841 attttcggta gtacgtagct cgactgatcc ctaatgcctc ccacggcttc agagaacgcg
     3901 ctgatgtagg aactgctaca cgcttggatt tagcaccgcc tatttttcca cctttgcgac
     3961 cacgcgcagc ctgtaccgca gaaaaacctt caggagagaa ttttcggtag gtgtatttag
     4021 caatactctt gccaatagcc cgacattcag ccggtgaaag cggaacgaga agcgatgcgt
     4081 tgtacatttc gacacgctga atcacggcat caagccactg tgagaatgct ggccaaccct
     4141 gacgaatcgc cctgtaagac catttacggc tcttttcgaa cagataccag tttcgcccca
     4201 tcccgtaatc tttatcgacg cgacggcgcg ctgaggcgct caaatcgaga taatccgcca
     4261 gttcatcgag ggtatagagt tccagacgcc attccacaac ctgccattcc tgatggaacg
     4321 gatttttgca gatcaggccg ctgtagttca catccgcgcc taatttttca cacagtgcac
     4381 gctcaatcgc agcagcgtat ttgagcgccg aagccgatgc atccggcgcg gttctcaccg
     4441 gaagagtgag tgcgtagagc agatgcgcgt gcccgttacg gggatttttt acggtgatat
     4501 tcggggccgg acagttccgg tcgctccagt ctatcgctgc gcccgtccgg tccacgtcaa
     4561 aaacaagcca gtgctgcgca tgtggctggt tctgctggat gaaacgggca aggatggcga
     4621 tatctttcgg cagaatgcga acgccgtacg tgaaatcgtt cgtacagtac ggtttgtgcg
     4681 gtaatttttc cctgaagcgt tgaagtacgg cgctcatgtg ttaggatttc ctctgtgtgt
     4741 tacaacccac acgccccttg aattttcgtg acagtcttgg cggatcggca cgaatttcag
     4801 gggcgtttgt ttttcagctg cttacactac acccaacgac aaaccctgtc gatccatcac
     4861 gaaaacccga tccatttacc ataactcccc ttgtacgtac cctctaaaac gctctcaatc
     4921 gctttctgac gcaaaaaact caaaaagaca agataaacct agggaaaaca caaaaaacgc
     4981 gtcagaatcg tttttagggg cctgcataac tatgcacaaa agcgattaat gatcggtttc
     5041 accccttcag aagcgcgaac tggtcaaaac agcaaacttt ctcatccaga aagccagctg
     5101 aactaacccg catgaaaaca aatttccaaa catatccagt ctggacaacg aaatatcgac
     5161 aatataaaac atcataatcg tgtttatggc gtgtaaaaaa gcttacaaga gaattagcat
     5221 tgaagagtca taacaacaaa ttccaaaaat caaaccagag gataggaaaa tgaataattt
     5281 tataaatgaa tgtatttccg ctccgagaat aaacggcatg gcagaatttt tctcatcaac
     5341 tgcatcaggg gaaaaacaca aaccttttag actagtagaa atagatgatc ggctttttca
     5401 ttcttatgat tacattaagt ttgccaaaca cctcacaaaa aacagcggcc ctttcgttaa
     5461 tcattatttt tgcagtatac catacagcaa ggaagaagag tgcagattag gaacaacagt
     5521 aataaaatac gcaatcacgc taaacaaaaa attaaacaca tggtgtcttg gtatggctga
     5581 gggcgcaatg gcaagaacca tttcagaatt atcaaaagga aacatacaaa cattaacaac
     5641 cagtccaaca aaagaaaatg aacatgcatt ttttaagtat
//
LOCUS       DAZFKF010000203         5654 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFKF010000203 DAZFKF010000000
VERSION     DAZFKF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.205"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            74..769
                     /locus_tag="ACHKA1_005154"
     CDS             74..769
                     /locus_tag="ACHKA1_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO5682681.1"
                     /translation="MTDLIAYKSNALVEASYKLTLQEQRLLLLCISRLNSSNATPESQ
                     KTMMITAEDFFNSFPDMGRNHAEEKLQEAIDRLWDRSIILKDDEKREEMRWLQYRAHY
                     LKGEAKVQITFADAVMPYLTQLKGQFTKVVIKNISRLSRSYSIRIYEILQQFRSTGER
                     VIALDDFRSMLQIDKKYKDFKTLNRSLIKPSIAELNQKSDLAVTVEPIKKGRTVVALR
                     FLFKEDKQIKAPI"
     gene            1140..2255
                     /locus_tag="ACHKA1_005155"
     CDS             1140..2255
                     /locus_tag="ACHKA1_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5682682.1"
                     /translation="MTKIYIYLVLAASLSALSTDMIVPALPELQKAFNADYSLIQLSI
                     SGFLIVFAFSQLISGTIGDHFGKVRVMTICLIIFLIGSIVCFMANNLNMLILGRILQA
                     IGAGAGPVISKAIAKESFPPLKLKRALSDISSTSATIPLIAPLAGALILDYYNWNVIF
                     IVMGSFSILTMLLSPKEANKQEKKTHQDDIKFVTKDFICGTILVSLMLSSLFCYISIS
                     PALFMNDYGLNTFNYSVVFSLSVLFFIIGNQLSKIEKISDPWVLFPLNAISVIPFLIL
                     NDNFVICVIGAMIFNLTLGAYYPIANFISLQINGSRTGLAASITGFTQTVSAGVISFL
                     SVKISDNGVSLGTTLSISCLILALLSIFVTIIGNNKK"
     gene            complement(2522..4741)
                     /gene="mobQ"
                     /locus_tag="ACHKA1_005156"
     CDS             complement(2522..4741)
                     /gene="mobQ"
                     /locus_tag="ACHKA1_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFO5682683.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTR
                     KRGVDDAVILTPENAPSWCADRSVLWNAVEKAEQRRDSQLAREIELAIPREISRDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKSACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTQLALRKVQETQRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFVAMNGKANDIKREGNALVKERDILEKEMTGLRKA
                     RPVASILSELPLMTWAEPEYRKRQLRFWKLGKQIESLSRTYRAVKERDIPARRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGA
                     VMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSDDRRPVASPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVG
                     FVRREGGFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
     gene            4920..5198
                     /locus_tag="ACHKA1_005157"
     CDS             4920..5198
                     /locus_tag="ACHKA1_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HFO5682684.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPRPPVKPQKND"
BASE COUNT         1480 a         1319 c         1307 g         1548 t
ORIGIN      
        1 aaaaaaaaaa tattgcactt atggtaaagg ttaattactt ttttttttgt taacctatca
       61 attggggaac gtaatgacgg atttgattgc ttacaaatcg aacgcattgg ttgaagctag
      121 ctacaaacta accttgcagg agcaacgctt gcttttgctt tgtattagcc gattgaactc
      181 cagcaatgcg acgccggaat cgcagaaaac tatgatgatt acggcagagg atttttttaa
      241 ttcatttcct gacatgggtc ggaatcacgc cgaagaaaaa ttacaagaag cgatagatcg
      301 tttatgggat aggtcgatta ttctcaaaga tgacgagaag cgagaagaaa tgcgttggct
      361 ccagtatcga gcacactacc tcaagggcga agctaaagtt cagataacct tcgcagatgc
      421 tgtaatgccc tatctgacac aattaaaagg tcaattcacg aaagtagtaa ttaaaaatat
      481 atcaagatta tcaaggagct acagtattcg gatctacgaa attctccagc aattccgtag
      541 cacaggagag agggttattg ctcttgatga ttttcgcagc atgctacaaa ttgataaaaa
      601 gtataaggat ttcaaaacat taaatagatc tttgataaaa ccatcgatag cggaattgaa
      661 tcaaaaaagc gatctggctg tgacggtaga gccaatcaag aaggggcgaa cggtggtcgc
      721 tctgcgtttt ctgttcaagg aggacaaaca gatcaaagct cctatctaaa tgcacttaaa
      781 acgctctcag acgcgttctg acgcaaaaaa cgcaaaaaga caaggaaaca ccaggccaaa
      841 ccgaaaaaac gcgtcagaat cgtttttagg tagatgcata actatgcacg aaagctatgc
      901 gcgatcaatt tcgaccgctc cgaagggcga acaggtcaaa atgacaagtt ttctccctcc
      961 agatggaagg ttcagcacgg ggatttttta cctccttcca tcaacctgaa tatgcatctg
     1021 gaagtaccca ttagtttctg ctcaaacact atcgcagtgc tatctcaaaa tataaaatat
     1081 accgatggtg taagtatttt aaatatgata aataagaata tttttatcag gggttaatta
     1141 tgacaaagat atatatttac cttgttcttg ctgcatcatt atcggcatta tcaactgaca
     1201 tgattgtgcc tgcgttacca gaactacaga aagcatttaa tgctgactat tcattaattc
     1261 aactaagtat tagtggattt cttattgtat ttgcatttag tcaactgata tcaggtacta
     1321 taggtgatca ttttggaaag gtacgcgtga tgactatatg cttaattata tttcttattg
     1381 gcagcattgt atgctttatg gccaataatt taaatatgct tattttgggt agaattctgc
     1441 aggcaatagg tgcaggtgct ggacctgtaa tatcaaaagc aattgctaaa gagtcttttc
     1501 caccactaaa gctaaaaaga gcactttctg acatatcatc gacaagtgcg acaataccat
     1561 taatagcacc tctggctgga gcattaatac ttgattatta taactggaat gtaatattta
     1621 ttgtaatggg gtcatttagc attctaacca tgctcctgtc acctaaagag gctaataaac
     1681 aggagaaaaa aacacatcaa gacgatataa aatttgtgac taaagatttc atctgcggga
     1741 caatactcgt ttcactaatg ctaagctcac tattttgcta catatcaata tcacccgcac
     1801 tatttatgaa tgactatgga ttaaatacat ttaactactc tgttgttttt tccctatcag
     1861 ttttattttt catcattgga aaccaattat caaaaatcga aaaaatatca gatccatggg
     1921 ttttatttcc tctaaatgca atatcagtca taccctttct catattaaat gataattttg
     1981 taatatgtgt tattggagca atgattttca atttaactct tggcgcatac tatccaatag
     2041 ctaattttat ctcattacag ataaacggtt cacgaactgg cttggccgca tcaattacag
     2101 gatttactca aacagtaagc gcaggggtaa tcagtttcct ttcagtaaaa ataagcgata
     2161 atggagtttc tttgggaact actttaagta tcagctgtct tattcttgca ttattgtcaa
     2221 tattcgtaac tattataggg aataataaaa aatgaattgg caataaagaa aaagctatca
     2281 caatatgcaa aaaaatgcat gatcgtggtt ttgctacaac cgttgctatg tatcctacag
     2341 tggaagaggg aaaggcgatt ctacgttgtg caatatctgc tattcatacc agagaaaata
     2401 tagatagcct acataaaaac tttatggaat gtattaacga accgggttag taatatcatg
     2461 gcctctttat atagaggccc tctatttcat tttttatggc ccgtggcgca gccctcgaat
     2521 atcacatact catgccccgg aagtgcccgg ggcggcgttg ttgttgcctg tgctgttcgt
     2581 cccgcttcca ctcatgccag gtctgattaa aaccgagttg acggaagaga taatccggca
     2641 tctcttccgg tttgtggaca gcagccaaaa gcctgtcatg atggcctttg agccggaact
     2701 gcatcaggtg cggttccggt gggtacggag tatcctcatc atggtttata gcttctccgt
     2761 aacctgccag gcataccccg ttctccgtga tggtgctgac tatcagatga ctgtaatccg
     2821 gcgacggaag cacaagaacg gggcaaaacc ctccttcccg gcgaacaaac ccgacaagca
     2881 tttcctccgg ccctttgcat aacaacagat tcagcgtcct gctgtttccc tctctcgaaa
     2941 atacaacagg catgagttgt tccggtacag catcagcggt gatatccacc cggtgcgctg
     3001 acggcagcgg agaggctacg ggcctgcggt cgtcgctgaa ataaaaatta tccggccagt
     3061 gatgcggcag cgccggaaaa ccgtaagagg gccacccctg gcgcatctgt tccagtacat
     3121 gccggaccgt cgcaccggaa tacattctca cacgctcagg ccgcgccatc gtctcatgag
     3181 attctggcat tccctcgcgt atatccgaga ccatcacagc cccgcctggc gacaggtaaa
     3241 cattcaccgg gtcatgaaca ctctgggggt aatgcgccag ctcatcaccc cataccgtga
     3301 attctccggc tctgttactc aacatcatgt agcgggttat cctgcccgtt cccggctctc
     3361 tggcacgccc cagctccgta ataaccccga ctaatgccgt ttcacggctc agacggtggt
     3421 tatcatgacg tttcagccgc gcctcatgct cctgttcctt ccggcgcgct tccgcttcgg
     3481 cttcctccct gcgccgctcc gcttcccgtg ctgacagttt ttctttcagc tctgccattc
     3541 ccggcgcaat ccacgtattc cactgcgttt caaaggcctg acgacgggcg ggaatgtccc
     3601 gttctttcac ggccctgtaa gtgcggctga gagattcaat ctgtttcccg agtttccaga
     3661 aacggagttg tcttttgcgg tattccggtt cagcccatgt catcagggga agctctgaca
     3721 ggatggacgc taccggacgg gcttttctga gtccggtcat ctctttttcg agaatatccc
     3781 gctctttcac cagcgcattc ccctcacgtt tgatgtcgtt tgccttcccg ttcatcgcca
     3841 cgaactcagg ggtcatctcc catgcgttcc cgcattcctg cggcagtgtg gcaatgaatt
     3901 cccgcagggt atccgctgag gcattcgcgg cgggaatggt cagattaagg gcttccgctg
     3961 cgcgacgtgc ggcatcgctg agttcccgct tacgctgcgt ttcctgaacc ttcctgagcg
     4021 ccagctgcgt gcgggaaacc tgtatttcca ggttaaggct gttaatcagg cggttctgct
     4081 caccgcgttc tgtttccatg ccccgttttt ccatcgcaca ggcagacttt ccgaggtgaa
     4141 ggcccggcgc tttctccagt ccctgacgct cgtatgaacg atggtctatc tcttcctggt
     4201 agccggcgtt agcaagcgct ctgttcgcat ggtcagccca tgatgcgcgc cacgtctcgg
     4261 cgagtgcccg gtcgttccag tccctgacct ttcctgcgaa tcccgtttcc ccgacagctc
     4321 ttgtggtcag catgatgtgc gcatggggat tagtccggtc catgtgatgg aacgccacat
     4381 cggcaatcat gccctggctg acaaagtttt ccctgacaaa agcgagaacg gtttcccgtg
     4441 cggcatcgcg ggaaatctct cggggaatgg caagttcaat ctcccttgcc agctgggagt
     4501 cccggcgctg ttcggctttc tcgaccgcat tccagagaac ggaacggtca gcacaccatg
     4561 acggtgcatt ctcaggggtg agaatgaccg catcatcaac gccgcgttta cgggtgtaat
     4621 cgtgggttaa tccggtacgg tcatcgggta tgacagagcc ggaacggtag gcagcagaag
     4681 caacggcact gtagccgttt tttcgcgaaa tgattttcat gctgagatga taaatcgcca
     4741 tttctgccgt aatgcctttt tgccgttttc tgtcagcccc gcagggcgca cacaatcgaa
     4801 acggagtgga gattgtataa gtgcgcactt cgtgatgtgc cgtgcattat gccagttcag
     4861 ttttgcaact accagtgacg cgctaaaatg cgatttttca ccgcgcacgg agtcacaaaa
     4921 tgagcagaac actcgaacag aagattgccg aagctgaggc cagattacag cgcctgaagg
     4981 cgaagagccg gagcctggac acggcgcaaa aagtcattgt gggggctgcc atgctggcga
     5041 gggtacggag gccggaagaa gcgcaactgc gtgcgttcct gctccagttt ctgcgaaagg
     5101 aagtcacaag acaggcggat gtgaaccgga tacaaccgct gattaacgag ctggagaagc
     5161 tacccagacc acctgtgaaa ccgcaaaaga acgattgatc gccttccgct cgccgcagcc
     5221 ccagcgaccg agcgttagcg agtaagtggg cgaggaagcg gcagaagtgc cgttgctgaa
     5281 tgtggcacgt acgcactgtt ttcagaaagg tgatcctctc tgaacctgca tgaatactgg
     5341 atttccacag gtttgcatgt tgtttttgca gaacgcggca aactgatttt ttataacatg
     5401 ttgtttttta gtgttttttt gttttcccgc tttgagtttt caacaggcac gccgttaagt
     5461 ttttagatct tgtttttata tacttgtttt tatacttgtt ttagggttga gatttctttt
     5521 ttaaatcaat atgttgcaat gacataggtg aacattttgg gggtataggt gaacattttg
     5581 ggggtggtta ggtgaacatt ttgggggtat aggtgaacat tttgggggtg gttatccaca
     5641 gaaaaaggtg aatc
//
LOCUS       DAZFKF010000204         5598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFKF010000204 DAZFKF010000000
VERSION     DAZFKF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.206"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..785
                     /locus_tag="ACHKA1_005158"
     CDS             <1..785
                     /locus_tag="ACHKA1_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFO5682685.1"
                     /translation="PLVQEAASLDSPLNAGEIRELRGRMADNERAISAPVTSVVPRIS
                     SLTVNLSPGASLPLVRTALHNVSVVTFTDINGSPWPQSDPPYNAAPGHFDVQFNGNMV
                     SVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTNTLSQEIDSRLDLRIPRQGPASP
                     VVAVPSEKIALHDATLQAFLDGVPPDDPTVRRLKFSGNVPGTTIWQHGDDLLVRSRAM
                     LRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSIHVTPELE"
     gene            789..2120
                     /gene="traO"
                     /locus_tag="ACHKA1_005159"
     CDS             789..2120
                     /gene="traO"
                     /locus_tag="ACHKA1_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFO5682686.1"
                     /translation="MSAEQDTAAKSGKKLSALLVLGGVIIIGGGYLLLTWLSPENSAS
                     LSSVNINSTTTGSGRTVVESQHYRDLLRADNERGAAEALRNNQTFIASLPRGLDTDHG
                     NNQRRDTQPADQAEGSGQPSTPPQADQTAREKQREALQKLLARINTQHPDARPPVVAA
                     AMWGTTGAPGISSHASPPGTGTSPQPRLQEASLATPAARTGIQLIPALTRVPAFIDTE
                     VDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGKGMDIHFDRMMWRGMFLKVNAYALN
                     EHTGMSSVASDVNHRWFKHIVLPSVLGGVGSVGSLYKDANTQVIQGNYGSITGRVGMP
                     DGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDISPPS
                     SGTAPSVPPPSGRPLTQEEAERQASDRIQAAVMRKQNEMRQRYGIQQESHP"
     gene            2117..2830
                     /gene="traP"
                     /locus_tag="ACHKA1_005160"
     CDS             2117..2830
                     /gene="traP"
                     /locus_tag="ACHKA1_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFO5682687.1"
                     /translation="MKNEHEDPGSFGETTTPASGKSFLKRQYMGYSLPVWAGCLLIIL
                     AAVWYKFLRAPSEPPADAGFAVTPPAAESWAEPMPMTETGSGNTMSAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKESITTLGDTIRKDEAWAQETRQLLDETRKQVHAL
                     QMQGPLQIPTGNNGQSTKTPPSRLQGMQIMSVEKGMAWIRWQGSAWAVREGDRLGNIT
                     ITRIEPSSRSVITSAGTLR"
     gene            2827..3357
                     /gene="traQ"
                     /locus_tag="ACHKA1_005161"
     CDS             2827..3357
                     /gene="traQ"
                     /locus_tag="ACHKA1_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFO5682688.1"
                     /translation="MMRIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIVICGMLVALPSFINAGGTQMGFRADSFGPIAYVQPTTFGAA
                     AGAANAMLSLVKLAGVGFAMNGISIWRKSLLDGHTALSSSESISKGNVKFVAGVLLVF
                     IDRVIDASLASLGLAF"
     gene            3404..3802
                     /locus_tag="ACHKA1_005162"
     CDS             3404..3802
                     /locus_tag="ACHKA1_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFO5682689.1"
                     /translation="MSRFRAMAARLCAAFIYVQLCLVACRDRLIRLLLLLPVILLPHT
                     AMANTLFDMIKTVGDGAKGSSSSFVAIAQVIGLVLFIAGLLGLKQIGKKPGAGLACIL
                     SIVVGACLAVVPEMMSSGQKTLGLSPVSIS"
     gene            3859..4110
                     /locus_tag="ACHKA1_005163"
     CDS             3859..4110
                     /locus_tag="ACHKA1_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFO5682690.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKTYEQNTATHRPGPGTLAFPAPATGHGIQR"
     gene            4025..4843
                     /locus_tag="ACHKA1_005164"
     CDS             4025..4843
                     /locus_tag="ACHKA1_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFO5682691.1"
                     /translation="MNKTPPLIAPARERWLFPLPQPDTVFSGESLLPPPVLSTPGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTVVLLEKNLTRVMRLKKHPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESELLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALKEIFSDERKRIRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILMPDSFPDKKYVM"
     gene            4898..5257
                     /locus_tag="ACHKA1_005165"
     CDS             4898..5257
                     /locus_tag="ACHKA1_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682692.1"
                     /translation="MDTLTAGYQFSADAFRRFIIRTLTDDIPPDGDEKEIARFILRHP
                     AFNGGWLLFLAAEIHERSRTHNSITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIR
                     RQQFIYPGVPPTKSGEE"
     gene            5257..>5598
                     /locus_tag="ACHKA1_005166"
     CDS             5257..>5598
                     /locus_tag="ACHKA1_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682693.1"
                     /translation="MKIHRISPETLITLILAHLAGKADSTAKEEHRLLRRFLRDDDGR
                     LAGILLNIAGILQFNRELSARHNYPATPLTEFSLRKRGKQLHLCLCSLRFFYIPPVFI
                     RNERRKCIVVHI"
BASE COUNT         1276 a         1657 c         1505 g         1160 t
ORIGIN      
        1 ctccccttgt tcaggaagcc gcatcactgg attcccccct gaatgcaggt gaaattcggg
       61 aactgcgggg acgaatggcc gataacgaac gggccatcag cgcacctgtc accagtgtgg
      121 tgccccgtat cagctccctc acggtaaatc tgtcacccgg ggccagtctg ccgcttgtcc
      181 gaaccgcact gcataacgtc agcgtggtaa cgttcacgga catcaacggc tccccgtggc
      241 ctcagtcgga tccgccttat aacgcggcac ccggtcactt tgatgtgcag ttcaacggca
      301 atatggtctc cgtcactccc ctgcgcgcct gggctgcagg gaatgtaacg gtctacctca
      361 aaggactgtc tgtaccggtc attctgaata tcaccagtgg tgaaaccgac accaataccc
      421 tgagtcagga gatcgacagc cgtctggatc tgcgcattcc ccgtcaggga cctgccagtc
      481 ctgtggtagc cgtaccatca gagaagattg ccctgcatga tgccaccctg caggcctttc
      541 tggacggtgt tcctcccgat gatcccaccg tgcgtcggct gaaattctcc ggaaatgttc
      601 cgggcaccac catctggcag cacggcgatg atctgctcgt gcgatcgcgg gccatgctgc
      661 gggatgaatt tgaacagacg ctggcatctg ctgacggtac gcgtctgtgg aaactgccgg
      721 tgactccctt actcaccttc tccgtgaatg gtcagtccat tcacgtcacc cctgaactgg
      781 agtaaatcat gagcgcagaa caggacacag ccgcaaagtc cggcaaaaaa ctgtcagccc
      841 tgctggttct cgggggcgtg attatcatcg ggggcggata cctgctgctc acctggcttt
      901 cccccgaaaa cagcgcatcg ctctcgtcag tcaatatcaa cagcacgaca acaggcagcg
      961 ggcggaccgt ggtggagtca cagcactacc gggatttact gcgtgctgac aatgagcgcg
     1021 gggccgcaga agcgttacgt aataaccaga cgttcatcgc cagtctgccc aggggactgg
     1081 atactgatca cggaaataat caaagaaggg atactcagcc tgctgaccag gccgagggtt
     1141 ctggtcaacc atcaacgccc cctcaggcag atcaaacagc ccgggaaaaa cagcgggaag
     1201 cgttacaaaa actgcttgcc cgcatcaaca cacagcatcc tgacgcccgc cccccggtag
     1261 tggcggctgc aatgtggggt accactggtg ctcccgggat aagctcacac gcctcaccac
     1321 ccggtaccgg cacatcacca caaccacgtc tgcaggaggc cagtctggca accccggcag
     1381 ctcgtacagg gatacagctg ataccggcac tgacccgcgt acctgccttc attgatacgg
     1441 aagtggactc tgacaatgct gtctcccgcg tggtggcctc cgtacctgcc ggcccatggg
     1501 ccggggccgt cctctacagc caggacgcca gactcgccgg caagggaatg gatatccact
     1561 ttgaccggat gatgtggcgg ggcatgtttc tgaaagtcaa tgcatatgcc ctgaatgaac
     1621 ataccggcat gtcatccgtg gccagcgacg tgaaccaccg ctggtttaag catatcgttc
     1681 tgccgtctgt gctcggtggg gtgggcagtg tcggaagtct ttacaaagac gccaacacac
     1741 aggtcattca gggaaactac ggcagcatta ccggcagggt gggaatgcct gatggcgagg
     1801 cgctggctgg cgtcattgca ggcggcatgg ccgaacgggg cagtcagttg ctgacccgcc
     1861 aggcggagag cgaaccttac cggcaggtga cagtgtcacg cggagaggtt gtctccattc
     1921 tgtttgttga gccggtcatg agcaacgata tcagtccccc ctcctccggc actgcccctt
     1981 ctgtgccacc gccctccgga cgcccgctca cccaggaaga agcagagcgt caggcgtctg
     2041 acaggatcca ggcagctgtc atgcgtaaac agaacgaaat gcggcaacgt tacggaatac
     2101 aacaggaaag tcatccatga aaaacgaaca tgaagatccc ggcagttttg gcgaaacaac
     2161 gacaccggca tccggcaaat ccttcctgaa acgccagtat atgggataca gcctgccagt
     2221 ctgggccggc tgtctcctca tcatcctggc cgcagtctgg tacaagtttc tgcgggcacc
     2281 ctcagagccc cctgcagatg caggcttcgc tgtcacaccg cccgcagccg aaagctgggc
     2341 agagcccatg cccatgacgg agaccgggag cggaaacacc atgtccgcgc ctcctgtcac
     2401 cccacaggca gacatgcagc agcttgcccg tgacctgaaa gcagagctgg acaggcggga
     2461 tgaggaaatc cgacgtaccc tgaccatgct gaaagagagc ataacaaccc ttggcgacac
     2521 catacggaaa gatgaggcct gggcacagga aacccgtcag ttgcttgatg aaacccgtaa
     2581 gcaggtgcat gccctgcaga tgcagggacc actgcaaatc ccgaccggga ataacggcca
     2641 gtcaacgaaa accccgcctt cccgcctgca gggcatgcaa atcatgtcgg tggaaaaagg
     2701 aatggcctgg atccgctggc agggtagcgc ctgggcagta cgggaaggtg acaggctggg
     2761 gaatatcacc atcacccgga ttgaaccgtc cagccgctca gtcatcacct ctgccggtac
     2821 attacgatga tgagaattga tgccctgaca gccatagaga actttgccag cggtatattc
     2881 agtgcaggca tggagtttct cttcacctgg ggagagctgc tggggattat cggcctgatt
     2941 ggtcatctga tgcgtgcccg ggctgaaggg cgtcacagta tgggaccggg aaaatttatc
     3001 gccggtatcg tcatctgcgg catgctggtc gccctgccat ccttcatcaa tgccgggggg
     3061 acccagatgg gattcagggc agattccttc ggccctatcg cttatgtaca accaaccacc
     3121 tttggtgccg ctgccggtgc cgccaatgcc atgctctcac tggtaaaact ggccggagtg
     3181 gggtttgcca tgaacggcat ttccatctgg cgtaaatccc tgctggacgg gcataccgcc
     3241 ctgagcagca gtgagtccat cagcaaagga aacgtcaagt ttgttgccgg cgttctgctg
     3301 gtttttatcg atcgcgtcat cgatgcctct ctggcgagtc ttggtctcgc cttctgatcc
     3361 acattcattt tcagtaattc agtctgttag ttaaggagac tatatgtcac gattccgtgc
     3421 catggcggcg cgtctgtgcg ctgcatttat ttatgttcag ttatgcctgg tggcctgccg
     3481 tgaccggctg atacgcctgt tacttctgct tcccgtgata ctgctgcccc acacagcgat
     3541 ggcaaacacc ctattcgata tgatcaaaac cgttggcgat ggtgcaaaag gctccagcag
     3601 ttcatttgtg gccatcgctc aggttattgg cctggtgctc tttattgccg gcctgcttgg
     3661 cctcaaacaa atcggtaaaa aacccggagc agggctggcc tgcatactgt ccattgtggt
     3721 cggtgcctgt cttgccgtcg taccggaaat gatgagcagt ggacagaaaa cgctcggtct
     3781 ttctccggtc tccatcagtt aatccacaac ggcaccggtt tcccggtgcc gtcttcacac
     3841 aatgaagagg accagaccat gtcctgtgag ctgccccctt tcaccctgct cagaacagag
     3901 cacggcaacg tgattgcccg cgacaaccgg gataatcagg agtttgtgtt tgcagaatgt
     3961 tattccaccg ccatcgcagc actgattctg gctgccgtca ccctgtatac agagagaaaa
     4021 acgtatgaac aaaacaccgc cactcatcgc cccggcccgg gaacgctggc ttttcccgct
     4081 cccgcaaccg gacacggtat tcagcggtga atccctgctg ccaccacccg tgctctcaac
     4141 acccggacgc tgtccctgct gtcgcaggac cgtcactcac cgcttcattc tggaggattc
     4201 atggccatta cagcagatgg ctgacacctg ccgggatacg gtcgtcctgc tggaaaaaaa
     4261 cctcacccgg gtgatgcgcc tgaaaaaaca tccggtaccg gaaaacgctg acgaaaaaaa
     4321 gaaacacacc cgaaccctgc aggatgcgga gcggtccctg gcacaggccc gcctgtcggc
     4381 caggcgactg gccctgcgtc atgtggaaaa gtcacaaatc gtcacaacag atgcactcag
     4441 cgaaaatgaa agtgaactgc tgcagcctga aggcccgccc tttcatctct gtgccttctg
     4501 ccacgcctgg cactgcctga acggctacgc tgccgcacag ggggtgatgg tctggctccc
     4561 tgatttgcat ccggcctcgg tagttgctct caatgcacgg gcactgaagg aaattttcag
     4621 cgatgagcgt aagcgaatca gacagggacg ggccgtactg aatgccctgg tacagaaccg
     4681 cctggccgtt gaggagaaat ttcgcacctg gcgtccggcg gattttgctg atgcactgcg
     4741 ccgctggccg ccggcacagc gtaaaacact gcgggagaaa atggacgggg tggcgctcat
     4801 tctgatgcca gattcgtttc ctgacaaaaa gtacgtcatg tgacacacac cgctgccggt
     4861 gacgttctgc cggccccaac tgacagagga acaaaaaatg gataccctca ctgccggata
     4921 tcagttttcc gcagatgcat tccggcgctt tattatccgg acgctgactg acgatattcc
     4981 gcctgacgga gatgaaaaag agattgcccg gttcattctc cgccacccgg catttaacgg
     5041 cggctggctg ctctttctgg ctgcagaaat tcatgaaaga tcccgtaccc ataacagcat
     5101 caccgtcaca gtgagcgtcg ataactatct ctcatggatg gatattgccg tgtccggtct
     5161 cccggaatta ccggatatgc ccttctgtga ttttttcatt cgccgccagc agtttatcta
     5221 tccgggtgta cccccgacaa aatcaggaga agaatgatga aaattcaccg catatccccg
     5281 gagacgctta taacactcat cctcgcacac ctggccggaa aagctgacag taccgccaaa
     5341 gaagagcacc ggctactccg tcggttcctt cgggatgatg acggcaggct ggccggcata
     5401 ctgctgaata ttgccggaat acttcagttc aacagggaat taagcgcccg gcataattat
     5461 ccggccacac ccctgactga attttcatta aggaaacggg gaaaacaact acatctctgt
     5521 ctctgcagtc tccgtttctt ttatatcccc cctgtattta tacgaaatga aagaagaaaa
     5581 tgtattgttg tacatatt
//
LOCUS       DAZFKF010000205         5442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFKF010000205 DAZFKF010000000
VERSION     DAZFKF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.207"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..185
                     /locus_tag="ACHKA1_005167"
     CDS             <1..185
                     /locus_tag="ACHKA1_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682694.1"
                     /translation="ADDAGSVAGVVHAVAGTDLAEAEAEAEAEAEAEAEAEAEAEAGQ
                     DAPWKKTGNKAKQARA"
     gene            182..601
                     /locus_tag="ACHKA1_005168"
     CDS             182..601
                     /locus_tag="ACHKA1_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFO5682695.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            598..1158
                     /locus_tag="ACHKA1_005169"
     CDS             598..1158
                     /locus_tag="ACHKA1_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFO5682696.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWNDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            1159..1404
                     /locus_tag="ACHKA1_005170"
     CDS             1159..1404
                     /locus_tag="ACHKA1_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO5682697.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            1401..2900
                     /locus_tag="ACHKA1_005171"
     CDS             1401..2900
                     /locus_tag="ACHKA1_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO5682698.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATEFWGRGSMIALMVAEFIAINPDAELYAIAQGSGT
                     GQAQACLMNIIGTASQDGVLHIYIGGRRYLLAVTKGQKGKELIDALVSVINADADAPF
                     SAYAGQVADNASGLKGSMGINARFTGECSVHDIRVNYHDGETTPGGLTIELGYPKTKA
                     TNPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWMAHTGT
                     FGEVQAFGAKRNDFLATCTFIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALPS
                     RMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLDV
                     ETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWVE
                     LGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            2909..3274
                     /locus_tag="ACHKA1_005172"
     CDS             2909..3274
                     /locus_tag="ACHKA1_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO5682699.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASATSTRVQ"
     gene            3289..3765
                     /locus_tag="ACHKA1_005173"
     CDS             3289..3765
                     /locus_tag="ACHKA1_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO5682700.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            3892..>5442
                     /locus_tag="ACHKA1_005174"
     CDS             3892..>5442
                     /locus_tag="ACHKA1_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO5682701.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGTGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRPVTPPLP
                     PSPVPPSPS"
BASE COUNT         1225 a         1407 c         1664 g         1146 t
ORIGIN      
        1 ctgctgatga tgcggggagc gtggcgggtg tggttcatgc agtggccggt acagatttag
       61 ccgaagccga agccgaagcc gaagccgaag ccgaagccga agccgaagcc gaagccgaag
      121 ccgaagccgg acaggatgct ccctggaaaa agaccggtaa caaagcgaaa caggcccgtg
      181 catgaattac gccactgaaa ccgatatgcg ggcgcgttac cgcgaggatt tgctcaggcc
      241 gttactggct gtgccccgtt cggatgagcc ggatacgcgc aagctgaacc gggcgctgac
      301 agatgcgtca gcccttatcg acagctatct gtccgcccgt tacacgctgc cgctggaggt
      361 tattccggcc gttcttgttc agcactgttg cgcgattgcc ttttattacc tgtgcgatca
      421 gcgagcctca gatcaggcgc gtgaccgtta ccgtgaggcg ctggcctggc tgaaagatgt
      481 catgaacggc aatgtgccgg taggtgtgga tacgaacgga gcggcccctg aatccgggga
      541 tttaccacag gttcagtctg atgcagcggt attcgggcgc aaccagaagg gcttcatatg
      601 attacggaaa ccgaacaggc atacatcgcc cgtatccgtg agtatttcgg gaatgaactg
      661 gtgtctgttg acacgcatcc cggcgactgg aacgacagcg tactgcgcac catgctgatt
      721 aacgccccgg cgatctacgt tgcctggctg ggtgccggtg aaggccgtac ccgtggtcgc
      781 ctggtcagtc actgggtgtt ctacgtcatc ggcgacatgc tcaacgggcg tgaggccagc
      841 cgtcccggat tgtatcagat tgtggcccgg ctgattgccg tgcttaacgg cttcagaaca
      901 gaaaaaacct caccgcttta ctttgaaaag gcggtcaacg gttacaccga aacccaggcg
      961 gacagtggtg cggtgatgta tgcgctttat ttctcctgcg aggaaatgat cgcaccgctg
     1021 accgatatca gctcgctgga cgacttcctg cgtcattacg aaacctttgt ggagccgccc
     1081 ggtacgccgc cgtttgaggc acatattgat ttgccgggga acacgacaac ggcggtcgaa
     1141 ccgccggagg aactgtaaat gaaaaccatc tttatcaaac ccgcgccggg acgcctgatt
     1201 cgtgatcccg acacaatgcg cccgctggca caggaggggg aggaaaagcc ctttacgccg
     1261 ttctggtgtc ggcgtcttga tgacggcgat gtcattcagg ctgaaaaagc cgctgaagag
     1321 gctccggctg tatctgcgga tgcaacgact gcgacagaaa aaccggtcgc ccaaccggca
     1381 tccgataagg agaaaccgca atgattaatt ttgacgggat cggtaatgac cgccgtatac
     1441 cgctgattga ggttgagttt aataactcca tggctgtcac cggcactccg gcacagcgtc
     1501 agtgtgtgct gctgtttggt caggcgagaa tgaaagagaa tgccgtggac ggtgccggtg
     1561 ttctggatgt tccggtcagg atcacccgtg cgtctcaggc aacagaattc tgggggcgcg
     1621 gctccatgat tgccctgatg gtggcggaat ttatcgccat caatcctgat gctgaactgt
     1681 atgccattgc tcaggggagc ggcaccggtc aggctcaggc atgtctcatg aatataattg
     1741 gcaccgcctc tcaggatggt gttctgcaca tttatatcgg cggtcggcgt tatctgctgg
     1801 ctgtcacgaa ggggcagaaa ggtaaggagc ttattgacgc tctggtctct gttattaatg
     1861 ccgatgctga tgcgcccttc agtgcttatg caggtcaggt agcagataac gccagcggac
     1921 tcaagggaag tatgggaatt aatgcccgtt ttaccggtga atgttccgta catgatatcc
     1981 gtgttaatta tcatgacgga gaaaccacac cgggtggact gaccattgaa cttgggtatc
     2041 ccaaaacaaa agccactaac ccggatatca cccgaagcgt ggcgggtatg ggcgatcgcc
     2101 agtacaacta tgtcgtcatg ccctataagg atgatgcaaa cctgaaaatc atcagtgatg
     2161 agcttctgaa acgctgggga ccggccaaaa tgtctgacgg tgtgctgtgg atggcgcata
     2221 ccggcacgtt tggtgaggtg caggcattcg gggcaaaacg caatgatttt ctggcgacct
     2281 gcacattcat tcccaaagcc cctgaaccgg attacctctg ggcagcggcg gtctgcgcca
     2341 cctgtgcgcc gtctctgggg gcagatcctg cgcgtccgct tcagacgctg gcgcttccgt
     2401 cacgcatggc cccggccccg gccgatcgtc tgacgcgtga agagcgtaac agtctgctgt
     2461 ggggagggat tgccacggtc agtgtggctg ccggtgatgt ggttcagatc gaacgtcagg
     2521 tgaccatgta tcgccagaac gtgtacggcg aaagtgatcc cagctatctg gatgtggaaa
     2581 ccatctacac cctctcctgg ctgcgttatt ccctgcgtac ttttatcaca caacgttttc
     2641 cgcgccataa actggctgat gacgggacgc ccgttcgtgc cggtcagaat atcgtgacgc
     2701 cggagatcat gaagcttcag ttaatttcgc tgggtgaaga gtgggtggaa ctcgggctgg
     2761 tcgaaaatct ggacaccttt aaaaagaatc tgcttgtgga gcgtaatgcg tcagatcgaa
     2821 accgtcttga cgtgttatgc acgccggatc tggttaacca gttccgcttc ctggcagcac
     2881 agatccgttt cattttgtga ggtaaggcat gagcggaaaa cagtatcagg gcacggccac
     2941 tatccgtgtg aacggtcagg aatacgagac gcttgagggg gctacatttt ctccgtcagg
     3001 ttttgaacgt gaagtggtga aaggcgcgaa agtctatggt taccgccaga aaccacgtga
     3061 agcgacactg gactgcaaat ttccggcagg cggagaaggt tcaccagccg ccgatgaaat
     3121 caacaactgg actgctgtca ctatcgagtt tgtggcggat accggcgaag tccacatgat
     3181 gacgaaggcc tggagcagtg aaccggcctc gcttgacggt ggcggggaga tctccgtgaa
     3241 gttcgccagc gccaccagta cccgtgttca gtgatcagga aaaataaaat gaccacacgt
     3301 aagaaaaaaa cggcagtttc cgaggcagcc gtgatggagg caatccggga agcacttgag
     3361 ggggctgatc cacgcactgc cgggctgaca gagcaactgg caaagggata tgtggatctg
     3421 cttgatggtc tgccgtttgg tgagacccgt gaatatcgtg tcacgttccg ggaactgaca
     3481 gcgaaagaca gtattgacgc agaagcagaa gccgagcgcg tggtggagac aaacaatggc
     3541 ccgatgctga tagcgtctcc gtcgttacgc ggtgttgcac tgctgcgccg tcagattgcc
     3601 gcagtgggag acattgaagg tccgttgtca ccccgtcaga ttggacagtt aagcgagcgc
     3661 gatctctccc gcctgatggc agcggtcagc ctgctggata ccgcgcttgc cggaaagctg
     3721 gcagctgacc gggggcgatc aggcgcagtg tcgggatcag attgaagaag cggcaattat
     3781 cctggggatg gtgacaaaaa gcggtccgga atgggcgctt aacctccccc tgtcgcaact
     3841 ttaccggcac tgccgacaga cagaaaaaat catcagaacg aagcagtaag tatggcccga
     3901 aatctcagag catctctgat agttgatttg ctcggcaata tctcagccaa atcccgccag
     3961 tggtcacagg agctgggggc attctcacgc tccggccagg cggggctggg tggcctggga
     4021 aatgctgccc gccgtgccgg gcaggaaaca gatgtactcg gaagcaggat gcagcgcacg
     4081 ctggccgggg tgcggggcag tatccgccat gtcacttctg attttgaccg tcttcagggc
     4141 agtattaccg gcaccatcgg gcggatcagc aatctttacg gaatgctggc cggtggtgct
     4201 gctgtatatg gctttaaccg gggatttatt cgcccggcag cggagatgga aacctatatg
     4261 attcgcctga actcccttta caaaggggat cgggcgaaaa ctgatgatgt ccgcaggtgg
     4321 gcaatacaga atgcgaagga aaccacctgg gggctggctg gcgtcatgca ggaatatacg
     4381 tccagtctgg gctttggtat gagtgacagg gaggcccgaa attttgtcac catgcttcag
     4441 gatcagggtg ccgtgggcgg ctggtcactg tccgatgcac agggggcatc cctgcaactg
     4501 aaacagatgt atgcaagagg cagtattcag gcagcggatg caaatattct tgccacctac
     4561 ggcattaatg cctaccgcgt gctggctgat cgtctgggtg tggaccagaa agtggtcaga
     4621 aaacttggtg aaaaagggct gcttgggccg gacagtattc gcctgttatt tcagacgctg
     4681 gcggaacagg ccaggggagc acaaaaggac gcaatgaatt cctggagtgg cctgacggcc
     4741 atgatgggtg acgtctggga tcagtttgcc cgggatgtga tggacagtgg gccatttgaa
     4801 aaactgaagg gtaatctcag gggatttctt tcatgggttg acagtgccaa atctgatggc
     4861 agttaccatt ccctggcgga aagtacggca tcagccatga accagggctt tgagtatgcc
     4921 cgtgatgcag tgacagggtt ttatcaggcg atcagaaagg tacgcgacac gcttcaggct
     4981 ctgcgggatg cgggctatgg tgatgcgctg gatcgcattg gtcagggggc acagaccgcc
     5041 gcgaaatacc tgctgtacat gtatctcgcc acccgtgccc tgaaaatggc gcgggctgtc
     5101 ggaacgggga ttatacgtcc gggtgctgca ttgctgggat acggattatc agcggcggcc
     5161 ttccttacct cacctttccg gcgctctcct ccggccggaa caccaccagg cacatctcag
     5221 ggacgagggc aacgttttat aaactttctt accggggtaa atccggcagc cgttcagcca
     5281 gtacttgtaa caaactggcc cacaggagga ctgtccggca ccggcgcgac cgatacatcc
     5341 tcaggcggac gtcaggggcg gggccgcagg aaacgcggac cggggcgcgg aagaccggtc
     5401 acgcctcccc tgccaccgtc cccagtgcca ccgtctccct ct
//
LOCUS       DAZFKF010000206         5410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFKF010000206 DAZFKF010000000
VERSION     DAZFKF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.208"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..266
                     /locus_tag="ACHKA1_005175"
     CDS             <1..266
                     /locus_tag="ACHKA1_005175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682702.1"
                     /translation="KNNTSKPPDQQVSHRLSEQQNRFAGGWSHCVTPTTITSVFSRTV
                     VNHKIGTHAGDALQNSCAFLVFTNQGMVCRSIAVSPETRSEHL"
     gene            complement(552..1550)
                     /locus_tag="ACHKA1_005176"
     CDS             complement(552..1550)
                     /locus_tag="ACHKA1_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFO5682703.1"
                     /translation="MKNPELKNLTDYSPEDKPWDERRSFSDDVGGIYLRAAGFEHYGE
                     RVGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPK
                     IVTEHPGARWLFLTLTVRNCSIDALGETLTAMNAGFQRLKDRKEFRHVLGWVRTTEVT
                     RGKDGSAHPHFHTLLMVPPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRK
                     PKDDESLPCVVAEQLRGAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGAL
                     KNVLQLERETNEDLIMADGMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRRLSSDD"
     gene            2392..2586
                     /locus_tag="ACHKA1_005177"
     CDS             2392..2586
                     /locus_tag="ACHKA1_005177"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO5682704.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAK
                     GENIDWCIARAEYCKKTGRF"
     gene            complement(4067..5083)
                     /locus_tag="ACHKA1_005178"
     CDS             complement(4067..5083)
                     /locus_tag="ACHKA1_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFO5682705.1"
                     /translation="MKNPDSPVLSLRDYSTRDTKWDSDRVMADRVAQIYERDSMFSSR
                     GERMFDCSRRLLFAPKVSRLTGEMKLALRKGEFCHVPFCPVCSRRRSLRWMRRLWEAL
                     PKLLAERPAARWLFMTLTVKNPPVEDTRETLIRMNAAWNRLVRRKEFRSVLGWLRTME
                     ITYGKVPGCCHPHFHVLMMVPPSMLSGNGYVKHARWVEIWSECLRVDYEAGVDIRVVK
                     PKQGWKRPDGVTLPDMHRAALESGVIETMKYTVKSSEVVRDPAWFLELARQTYGLRMV
                     ATGGRLKEVLKVDKPETDEDLIGADIPAELDEFEEQAFWLAFDWGRTEKRYKRNPEAD
                     RMKD"
BASE COUNT         1471 a         1482 c         1161 g         1296 t
ORIGIN      
        1 aaaaaaacaa cacatccaaa ccacctgatc agcaggtttc tcaccgactt tctgaacaac
       61 aaaaccgctt tgcaggtggt tggtcccact gcgtgacacc aaccaccatc accagtgttt
      121 tttctcgtac cgtcgtcaat cacaaaatcg gaacccacgc cggagacgcg ctacaaaact
      181 cctgcgcgtt tctggtattt actaaccagg ggatggtttg ccgtagcatc gctgtgtcac
      241 cagaaacgcg ctcagaacac ctctgacggc ctgaaaacaa aaaaacgagc aaaaccttgc
      301 ccgcttcctg aaatcgcgtt acaaagcgtc tgagcgcgtt tcttcctcgt tcaatctgtt
      361 cagttcagtc attccattgc ctagaaaacc tctggaaacg cgctcaaaac gtctctgacg
      421 gcctgaacca accaaaacat acaaaaccat tgcccaatgc aaaaaactgc gttagaaagc
      481 gtctgagcgc gtttctggtg agcaaaaagc tgtcagccgt ggatttccat tgcagagacc
      541 cagaaacgcg ctcaatcatc gctgcttagc cgacgtttat ctttcgcccg atcacgctgg
      601 tattttttga caaccggcac ccacgaaaac gccagacggg aaccgtcatc tgtaccctct
      661 gccatgccat ccgccatgat taaatcctcg ttcgtttccc gctccagttg cagaacgttc
      721 ttaagcgctc cccccgtagc aacaaaacga cgcttgtgta cctgccgtgt taattcgagg
      781 aaccattcag gatcagccag catatcagca ggctttgtgg aatatttcag agtttcaacc
      841 accgcgccgc gcagctgttc cgcaacaaca caaggtaaag attcatcgtc ttttggtttc
      901 cgtgacttca ccactctgat atccacattc gggtcatacg ccacccgcag acaatcacgc
      961 cacagttcca cccaacgggc atgtttcaca tactcacggc cggacaacat cgagggggga
     1021 accatcagca gagtgtgaaa atggggatgt gcgctaccgt ccttgccgcg cgttacttcc
     1081 gtggtacgca cccatcccag cacatgacga aattccttgc gatctttcag ccgctgaaaa
     1141 ccagcattca tcgcggtcag cgtctcacca agcgcgtcaa tggaacagtt gcgcaccgtc
     1201 agcgtgagaa acaaccaacg cgcgccggga tgttccgtca caatctttgg cagggactga
     1261 tagaaacggg cttgccacat catggagcgc ctccactggc agatcggaca gtgccggaca
     1321 cgacagaacc gcgcatcacg caaccgcagg cgcgtttctc cggtctctgt gtttgttgac
     1381 cacccaaacc gcaaaatacc actacaagcc ccgacacgtt cgccgtagtg ctcaaatccg
     1441 gcagcacgca gataaatacc gcccacatcg tcggaaaagc tccggcgctc atcccacggt
     1501 ttatcctccg gggaataatc cgtcagattt ttaagttcag gatttttcat gtctgccgtt
     1561 ccctagtgag cgacagacga aattacgtgt tatcattcct gtatcacctg tgttgtcgtg
     1621 tcaccaattc gaattcctac aggtggtcac acccccgaac gacgcccatc gttgcggggg
     1681 tcgcttttaa tgcgaccact tccgacaaaa tactctcaaa cctatttcca cacaaatccg
     1741 cgctcagccc agatgtgaca agggctagag ctaaattaca atcccagaga tatcccttaa
     1801 gtatatatac aaggatcgcg cgcacgatca cccaccaaaa atgcgcgttt tttgagcgct
     1861 tttccgcaca acaaaacaac acacaaaaac cactctcccg acaatactcc agtaggtttt
     1921 ctcaccgatt ttccgaacaa caaaaccagc tttgcaggtg gttggtccca ctgcgtgaca
     1981 ccaaccacca tcaccagtgt ttttttctcg tgccgtcgtc aatcacaaaa tcggaaccca
     2041 cgccggaggc gcgggacccc acgattttct gtttgacgag tcagaaaacg gcgaacgctg
     2101 acagatttca atctggctgc gtgagacgtt ggcggggtgt cggggcgcag ccctgaccag
     2161 gtggcatttg tctgattgcg tgtgcgcggt ccgacaaata cacatcctgt cccgtttttc
     2221 gcacggtttt tattgattac cggacagatg aacccggatt gccatgttgc gaaaatcacg
     2281 ttaaaacgat tacgagcgtg cagttggact gatatatcaa cagtcatgcc aatatgtatt
     2341 aacccgacag gccgctcttt tttaattcaa ataaaaatat aaattacgag tatgaatcag
     2401 gacaaagcaa tttatttaga aaataaatat ttttcacccg caaacgaagc tgtcagactg
     2461 gagcgtgaag gcgattacat tggagcatgg aaaaaatgga atgatgcgct tcattatgcc
     2521 aaaggagaaa atatagactg gtgtatagcc agagctgaat attgtaaaaa gacaggccgg
     2581 ttttaattct gacggggttt caaaggggcg tcccctttgg cacgtcgacg tatcaaaatg
     2641 cgccagcatt ttgtatacag tcggtaacgt gcctccagga tttaccatca aaaacgcccc
     2701 tgtagttaat cctgaatcaa gatataacta atatataact aatgtataac taacggttca
     2761 ccaacggatc gcatgtagta gacgctacat aaaaaatcct gaaatggagg attcgtcata
     2821 aagagattcc tgtttttagc gttctgatgc aggatagggc aaacaccgca aaatgacgtc
     2881 tttggtatgt tttgtgatgt ttgcttctgg catctgttat ctgttttatt gggtgttttt
     2941 atgagtgttg atgtaattaa acaaattcag agtatacagg acaaaggaat tattatttat
     3001 tcaaaataca gagctaacga atttaacagg gatgatgtat acagagagag ttacagcctg
     3061 attattgagt ttaatcatct tattgctgaa catgccgttc atgatgaaaa actgattaac
     3121 catactgtct gtattctgaa tgaattacga agcatagcga taaattaaaa aatccccctg
     3181 gattacaggg ggaagccatc agagtttgag taatgagaaa tattattttg gagagagcac
     3241 cacaaatgcg ctcttttcca atctgccgca accatcagca aaagacaatc agaacaggcg
     3301 aaaaaatccc cctatatggc aagggggtgt caatgaggac atggcaattg tgagttttgt
     3361 gtgttttatg tgttgcagtt tgcaacgtca gaaatttgcc gcagttatgc gcgaaaaaca
     3421 atcctgattc cgggacagga tgtgtaatcg tataaccgcg cacgcacggc tatacgatta
     3481 ccacctggtc agggctttgc ccgacaccct cgacaccgtt cccgcaatac gatagaacat
     3541 cagggcgcgt ttccgggtaa acgcatgtta atgaccggga attcattgat tagggaccgg
     3601 agacgcgcta caaaactcct gcgcgtttct ggcatcaact gaccatgggg atggtttggc
     3661 agtagcagca agcgccagaa acgcgctcaa aatcatcaga tgtacagcga acaaaaaatt
     3721 gtcatctgca aaaaatcacg ttaaaacaat cgcgagcacc ttcctgtctc cttcaattac
     3781 ggtcaaacat tttgcatctg tcgggaagtt gctcacctcc ataattcaat ttctcattga
     3841 caataattcc ggtgatatcc cctcttttga ggggcttttt ttacccacac accacaaaca
     3901 gcagccatac catcgtcaaa aacgcgctca aaacgtctct gacgccctga accaaccaaa
     3961 acatacaaaa ccattgccca atgcaaaaaa gtgcgttaga aagcgtctga gcgcgtttct
     4021 gacgtctttt ttgttgctgt gtcgctactc gttgtcagat tcgcgctcaa tccttcatcc
     4081 tgtcagcttc aggattccgc ttgtaacgtt tttccgtacg tccccagtca aacgccagcc
     4141 agaaggcttg ctcctcgaac tcgtccagtt ctgccggaat atctgccccg atcaaatcct
     4201 cgtccgtctc cggtttatcg actttcagaa cctctttgag acgaccgccg gttgccacca
     4261 tgcgaagccc ataggtctgc cgcgccagct ccagaaacca cgccggatca cgcacaactt
     4321 ccgaagattt caccgtgtat ttcatcgtct caatgacgcc tgactccagc gccgcacgat
     4381 gcatatcagg cagggttaca ccatccggcc gtttccagcc ctgtttcggt tttaccaccc
     4441 gaatatccac acccgcctca taatcgacac gaaggcattc actccagatc tccacccagc
     4501 gagcatgttt cacataacca ttaccggaca acatcgaagg ggggaccatc atcagaacgt
     4561 gaaaatgcgg atggcaacat cctggcacct tcccgtaagt aatttccatc gtacgtaacc
     4621 agccaagaac tgacctgaat tctttacgcc gaaccaacct gttccacgcc gcattcatcc
     4681 gaatgagtgt ttcgcgggta tcttccacag ggggattttt gacagtcagt gtcatgaaca
     4741 accagcgggc cgcaggacgt tcggccagca gctttggcaa ggcctcccag aggcgacgca
     4801 tccagcggag agagcgacga cgactacaca ccggacaaaa cggaacatga caaaactccc
     4861 ccttccgaag ggccagcttc atttcgcccg tcaggcggga caccttcggc gcaaacagca
     4921 accgacggga acaatcaaac atccgttctc cacgagagga aaacatcgaa tcgcgttcgt
     4981 aaatctgcgc caccctgtca gccatcactc gatcactgtc ccattttgta tcccgcgtgc
     5041 tgtaatcgcg taacgagagt accggactgt ctggattttt catttgcacc ccccaagaac
     5101 tgggcagatg cggaaatttt gtgttagcat ttttctgtca cctgtgactc tgttgccgaa
     5161 caacgaattc caaactggtg atcgtcccct ggagtgttac cgcactcgca ggggaacccc
     5221 cctcaaaatc gttcattttt taaccagaag tttttatctc ttttccaccc atttttaccg
     5281 ctcaaagccg cgccaccact gggctaagcc aaaataacac atccttgagt tgttgcctat
     5341 agtataaata caaggatcgc gcgcgcgatc gcccacccaa aatgcgcgtt ttttgagcgt
     5401 tcccgagaac
//
LOCUS       DAZFKF010000207         5373 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFKF010000207 DAZFKF010000000
VERSION     DAZFKF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.209"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            304..681
                     /locus_tag="ACHKA1_005179"
     CDS             304..681
                     /locus_tag="ACHKA1_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682706.1"
                     /translation="MYSPLIAHYSALQTQSALLAHISQMEGELMRLRAWQRLGITPEP
                     DDETEPSLVYVYLWDTGEALGWLLYDLEQEEISAPGIQARQALDSLMALGREINHEIW
                     ADSISTGKMVAGADEYFARHDMM"
     gene            763..1392
                     /locus_tag="ACHKA1_005180"
     CDS             763..1392
                     /locus_tag="ACHKA1_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682707.1"
                     /translation="MSELSSRPAREPVVYTLEQVSTIPEKQWHAFVLAVTETFWQLPE
                     ALRPQNAYFGSLTRASELFPVTDTLAFYCRSADGLWSVNVTIEREHSRNILALNELNF
                     GRQPGDFFARTVFVLLHNLCPDCFRIHSTVGGASWSLPLKWIKRYLGHENFSAPESVL
                     TPPVRGDAFDSLLLQFLSGQGRQLSPDDWAALEEAEYQLYWLRALAGGR"
     gene            1508..1891
                     /locus_tag="ACHKA1_005181"
     CDS             1508..1891
                     /locus_tag="ACHKA1_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682708.1"
                     /translation="MGLDIYFLARDNHHSGAVVGTQEHQEVGAFRKVKPLLTWFREHT
                     GASDEQHAFPVTRSSLAALLRDLSMLTPGNCHRLFPYVTGSCLRGEDYAGDYWYDVDD
                     VKAWVSNTLKTFDFERRSLVMLALW"
     gene            1984..2961
                     /locus_tag="ACHKA1_005182"
     CDS             1984..2961
                     /locus_tag="ACHKA1_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFO5682709.1"
                     /translation="MKTKKHTSRTATKTPERDLYQLVTDRVVTAIENGVPPWKRPWRS
                     ARNGVGLPEIMPANALTGKPYTGVNVLLLWLAADEAGYVSNDWLTYRQAQQLGGHVRK
                     GEVATLAVIYKDWKKQAVDEHGNRLTDSEGEPLMETVPVLKSNPLFNVGQCDGLPEHL
                     MVKAEGSADALPADTFSERVLQVLNGSGVSVSSLPQDRAFYRPDLDRIVMPLSSQFDQ
                     VADYQATLLHELVHSTGHEKRLRRVGISTFTGRKSPEYAFEELIAEMGSAFLCAHLGI
                     AGDVQHESYIDGWLKVLKSDKKALFRACRQAREASEYLLTRRTSQECAA"
     gene            3071..3505
                     /locus_tag="ACHKA1_005183"
     CDS             3071..3505
                     /locus_tag="ACHKA1_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD29 domain-containing protein"
                     /protein_id="HFO5682710.1"
                     /translation="MNDNALVDVDDAVVARIRDVLAYTFPGQKFSVRKCDPSFPKVFN
                     NVWVSWLDGPTEAEVRSVTEGFENKEGLQSVLESRYFSDELVQECIDRLRKEYGQGNV
                     PLSVTVERYRKNELEKIETARFPGNISVAINEIGINTSKYRR"
     gene            3509..3823
                     /locus_tag="ACHKA1_005184"
     CDS             3509..3823
                     /locus_tag="ACHKA1_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682711.1"
                     /translation="MAGKCLVWFCNGISEPCLIAGNISELLADVTITSNYRGDVTSEI
                     RDVVQEWLRTGRGNLADLKAELWYYNLFINPSADELMDVNRRYGPERTAWLQGFIRNN
                     GA"
     gene            3907..4854
                     /locus_tag="ACHKA1_005185"
     CDS             3907..4854
                     /locus_tag="ACHKA1_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO5682712.1"
                     /translation="MSEWCKNRFEITGKSVCLDVLTQWIEGRETPRYRHAIQQSIQLF
                     LAGCAGLVKPTRTTQYPPYPALVRQGTGLSSPANQAFEQWLGLLVKNAVLDGETIKTI
                     ERLYHQSCIGAVRWGNLPDNAREIITTLMSRQYSDWFGLAGLSEHIDAEACWSWLSDY
                     PEQAQPCDMLMVIPSRLATELNGSGGLLQGISTTTSLYSRIYGVEWPSGNNVRHARDE
                     MSSLVLLIDTPWYPPSGELVGEISKVFDCEVRHWYSEPVHGIQGYNCYDGGEHTDSDL
                     QAEWPGRETLPQPRLYLVEECAEEQTKAAPLPVPLVSAQ"
     gene            4966..>5373
                     /locus_tag="ACHKA1_005186"
     CDS             4966..>5373
                     /locus_tag="ACHKA1_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFO5682713.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSVASMMAQMQMGNVKALFESKPFITLSE"
BASE COUNT         1207 a         1236 c         1591 g         1339 t
ORIGIN      
        1 tgcaatccat gggagcgtgg atggaacgat ggctggacgg aatgtctgaa tcaacgtata
       61 gccgggctcc tgaagcaggc ctgtattgaa ttttccgacg cagactgacg ggcaggatgc
      121 cagtcagtca caggaaggat gtcaaatcgt gagcgatgca actgccattt tgcaatgatg
      181 gctgttttgc tcccgtaacg gtttttcttt tacttactgc accgtgtttt tgcgccggtg
      241 ttttccctga cgggagtttt gttccgtcag ggacaggact tcctttaaga gacggaggtc
      301 atcatgtatt cacctttaat tgctcattat tcagcgcttc agacgcagtc tgctctgctg
      361 gctcatatca gtcagatgga aggcgagctg atgcggttac gtgcctggca acggctgggt
      421 attaccccgg agccggatga tgagacggag ccttcactgg tatacgtgta tctgtgggat
      481 accggcgagg cgctgggctg gctgttgtat gacctggagc aggaggagat ttccgcaccg
      541 ggaattcagg cccgtcaggc actcgacagt ctgatggctc tggggcgtga aattaatcat
      601 gaaatctggg ctgacagtat cagtaccgga aagatggtgg ccggagcgga tgaatatttt
      661 gcccggcacg acatgatgta attcatgacc tgaccctgac gggggagttc tccgtccggg
      721 agacattccc cgttgtttag ttttaaccat gaggaatgtt ccatgtctga gttatcttca
      781 cgtccggccc gtgagccggt ggtatatacc cttgagcagg tttccacaat accggaaaag
      841 cagtggcatg cttttgttct ggctgtcact gaaaccttct ggcagttgcc ggaggcgtta
      901 cggccgcaga atgcgtattt cggctccctg acccgggcgt cagagttgtt tccggtaacg
      961 gacacgctgg cgttttactg ccggtctgcc gacgggctgt ggtcggtgaa tgtcactatt
     1021 gagcgggagc acagccggaa tatcctggct cttaatgagc tgaatttcgg acgtcagccg
     1081 ggagatttct ttgcccgtac agtctttgtg ctgttgcaca atctgtgtcc ggactgtttt
     1141 cgtattcatt ccactgtcgg tggtgccagc tggtcgctgc cgctgaaatg gataaaacgg
     1201 tacctggggc atgagaattt ttctgctccg gagtctgtat taaccccccc ggtaagggga
     1261 gatgcgtttg acagcttact gctgcagttt ttgtccggtc aggggcgaca gttatccccg
     1321 gatgactggg cagcactgga agaggccgaa tatcagcttt actggctgcg ggctcttgcc
     1381 ggtgggcggt aacagaacgc cgtagtttct ttatccggaa taacgtcatc acgtattccg
     1441 tcattatatc acccggcagg gacatatccc tgtggggaga gtcccggctt tattcaggag
     1501 aattgttatg ggactcgata tttactttct ggctcgtgat aaccatcaca gtggtgccgt
     1561 tgtgggaaca caggaacatc aggaggtggg ggctttccgt aaggtgaaac cgcttctgac
     1621 atggtttcgt gaacataccg gtgcttcaga tgagcagcat gctttccctg taacccgcag
     1681 ttcactggct gcattattgc gcgacctcag tatgctgaca ccggggaact gccacaggct
     1741 gtttccttat gtcacaggct cgtgtttacg gggggaagat tatgccggtg attactggta
     1801 tgacgtggat gacgtaaaag cgtgggtcag caatacgctg aagacgtttg attttgagcg
     1861 tcgttcactg gtgatgctgg cgttgtggta attctggcca gtctgatttt cgttattaat
     1921 ttaactgtac ggtgcactct caccgtacag ggagcgtgca tctttttcag ggggcatgct
     1981 cttatgaaaa cgaaaaaaca tacttcacgt accgcgacta aaactccgga acgtgacctg
     2041 tatcagcttg tgaccgacag ggttgttacg gccattgaaa atggtgtgcc accctggaaa
     2101 cgtccctggc gctctgcgcg taatggcgtg gggcttccgg agataatgcc ggcaaatgcc
     2161 ctgaccggga agccttatac aggggtgaac gtcctgttgc tgtggctggc cgcggatgaa
     2221 gccgggtatg tcagcaatga ctggctgacg taccgtcagg cgcaacaact gggtggccac
     2281 gtccgtaaag gggaggtggc cacgctggcg gtgatttaca aagactggaa aaaacaggct
     2341 gtggatgaac acggtaaccg gctgacggac agtgaggggg agcctctcat ggaaaccgta
     2401 ccggtactga aatcgaatcc tctgtttaat gtggggcagt gtgacggttt acctgaacat
     2461 ctgatggtga aggcggaagg cagcgcggat gcactgccgg cagatacctt cagtgagcgg
     2521 gtgctgcagg tactgaatgg cagcggcgtc agcgtctcgt cacttccaca ggacagggca
     2581 ttctaccggc ctgatcttga ccgtatcgtg atgccactgt cgtcgcagtt tgaccaggtg
     2641 gctgattacc aggccaccct gctgcatgag ctggtacaca gtaccggtca tgaaaagcgc
     2701 ctgcggcggg tggggataag tacctttacc ggtcgcaaaa gcccggaata tgcgtttgaa
     2761 gagctgattg ccgagatggg cagtgctttt ctgtgtgcac atctcgggat tgccggtgac
     2821 gtacagcacg aaagttatat tgacggatgg cttaaggttc tgaagtctga caaaaaagcg
     2881 ctgtttcgcg cctgccggca ggcacgtgaa gcatcagagt atttactgac gcggcgaact
     2941 tcgcaggagt gcgcagcata atttttacta ccggtggcgg ttataacctg accgccattt
     3001 atcccgtgag ggcattgtcc ctcacggggc agtgctctct gttatttcag gattacagag
     3061 gaactgagtg atgaacgata acgcactggt tgatgttgac gatgctgttg tggcccgtat
     3121 cagggatgtg ctggcgtata catttccggg acagaaattt tctgtgcgga aatgcgatcc
     3181 atctttcccg aaagttttca acaatgtgtg ggtgagctgg ctggatggtc caacggaggc
     3241 cgaagtacgc tctgtcacag aggggtttga gaataaagag gggctgcagt ctgtgcttga
     3301 atcccgttat ttcagcgatg aactggtaca ggagtgtatt gatcgccttc gtaaagagta
     3361 cggacagggg aacgtcccgt tgagtgtgac cgtggagcgt taccggaaaa acgagctgga
     3421 gaagattgaa acagcgcgtt ttccgggcaa catatccgta gccataaacg agatcgggat
     3481 aaataccagc aaatacagga ggtaaggtat ggctggtaaa tgccttgtct ggttctgtaa
     3541 cgggatctct gaaccgtgcc tgatagcagg aaatatcagt gaattactgg cagatgttac
     3601 gatcacgtca aattaccgtg gagatgtcac atctgaaatc agggatgttg tacaggagtg
     3661 gctcaggacc ggcaggggga acctggcgga tttaaaggca gagctctggt actacaacct
     3721 tttcatcaat ccgtctgcag acgaactgat ggatgtcaac aggcggtatg gccctgagcg
     3781 aactgcctgg ctgcagggtt ttatccgcaa caacggggcg tgataccccg ttttcccgtg
     3841 agggcattgt acctcacggg acaatgccct ctttttacat ctgtataaaa aagaggagcg
     3901 taaacaatgt ctgaatggtg caaaaaccgt tttgaaatta ccggtaaatc agtctgtctt
     3961 gatgtactta cacagtggat tgaagggcgt gagaccccgc gctaccgtca tgccatacag
     4021 cagagtatac agctgtttct ggccggttgt gcagggctgg taaaacccac acggacgacg
     4081 cagtatccgc catatccggc gctggttcgt caaggaacgg ggttgtcatc tccggctaac
     4141 caggcgtttg agcagtggct ggggttgctg gtaaaaaatg ccgttctgga tggtgaaacg
     4201 ataaagacca ttgagcgtct ttatcaccag tcatgtatcg gcgctgttcg ctggggtaat
     4261 ctgccggata atgccaggga gatcatcacc acgctgatga gccgtcagta ttctgactgg
     4321 tttggtctgg ccgggctgag tgaacacatt gatgcagaag cctgctggag ctggttgtcg
     4381 gattacccgg aacaggcgca gccctgtgac atgctgatgg tcataccctc ccgtctggct
     4441 acagagctga atggttcagg tggactgctg cagggtattt ccacaacgac cagcctttac
     4501 agccggatat acggtgtgga gtggccgtca ggaaataatg ttcgccatgc gcgtgatgag
     4561 atgagcagtc tggtgctgct gatcgacacg ccctggtatc cgccttcagg cgaactggtt
     4621 ggtgagatct cgaaggtgtt tgactgcgag gtccgacact ggtacagcga gccggtacac
     4681 ggtatacagg gttacaactg ttacgatggc ggagagcata cggacagtga tctgcaggcc
     4741 gaatggccgg gcagggaaac acttccacag ccccgactgt acctggtgga ggagtgcgct
     4801 gaagaacaga cgaaagccgc acctttaccg gttcctctgg tctcagccca gtaattaacc
     4861 tccgggtatc atgtccgtga tgcccgtttt atcaacattt caacccgaag gggaacacct
     4921 gttcccctgt ccgggccatg tgttccctgt ttttacggag aaaacatggc caccatgact
     4981 gaccatcaga aggagttcat ttcgttgttt aaccggacag cctgttatca caaacgtcat
     5041 aaggtgtttg aagactttgt cagctgtagt gtgattgccc tggaaaaccg gcttcagttc
     5101 agtgaggccc gggaacagaa gtatctgcgg atcgtcagtg gttatgaaaa gcaggatgtt
     5161 atccacattg cgcatttgct tgctcatgtc attgagggac tggagcaggg attctgtgat
     5221 tttctgggca gtgtgttcat gcaactggaa ctgggggata aataccgtag tcagtttttc
     5281 acgccatgga gtgtggccag catgatggca cagatgcaga tggggaatgt gaaggcgttg
     5341 tttgagagta aaccctttat cacgctcagt gaa
//
LOCUS       DAZFKF010000208         5057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFKF010000208 DAZFKF010000000
VERSION     DAZFKF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.210"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(183..338)
                     /locus_tag="ACHKA1_005187"
     CDS             complement(183..338)
                     /locus_tag="ACHKA1_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5682714.1"
                     /translation="MKQQKAMLIALIVICLTVILTVLVARKDLCDVRIRTGQTEVAVF
                     VDYESEK"
     gene            complement(538..963)
                     /locus_tag="ACHKA1_005188"
     CDS             complement(538..963)
                     /locus_tag="ACHKA1_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5682715.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIMQGIKLEMDRYVERERSGAQVSQFSGHTGN"
     gene            complement(1042..1905)
                     /locus_tag="ACHKA1_005189"
     CDS             complement(1042..1905)
                     /locus_tag="ACHKA1_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5682716.1"
                     /translation="MRTDNQVHKALFTIPTAAYSAVPANIKPLPEQRRITGHKQTDAY
                     LWILEVIHLNEAVHLDAAEAALEKLKITPEEASERYGRYLQEINVDPFQIAFATIGID
                     NPAQAIRNARENIKKAASVRATFGSYEAALDDVEAERIIRTSPKFIDDYYWGWTAAEK
                     KAGSIDGVRSNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFIYWDWLYEMRQTAGRET
                     GDKYGFTGEHHESVYDRQFWLENLLGKIKPVTRDEAVEVCRWFLASGKDEYMEDNGSA
                     VILNLVGECEQ"
     gene            complement(1905..2873)
                     /locus_tag="ACHKA1_005190"
     CDS             complement(1905..2873)
                     /locus_tag="ACHKA1_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO5682717.1"
                     /translation="MKTAEAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRI
                     DDRDGTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSES
                     LRSRIQRRFSALTSLQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALAT
                     DDKGELCYLHQTLLDGAKKADIGISARRLKSLQDDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYKVNTWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACEC
                     AHANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
     gene            complement(2875..4533)
                     /locus_tag="ACHKA1_005191"
     CDS             complement(2875..4533)
                     /locus_tag="ACHKA1_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO5682718.1"
                     /translation="MLNIKPNFAQERALNMLRHDWKTYQSFMMYMPTGSGKTGLAAFI
                     TAGLVSRGMRVLFVVPYTILINQTAQRFVQYGLSGDEISFIWRDHPNNDPSRLIQIAS
                     ADTLIRRDFPQNIDLLIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRY
                     YQRLLKPTTIGELIQRGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCG
                     ADLVGDIVDNWLCNGRDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQL
                     MISRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKES
                     CLIFDHSGTVHRLGFPENIEYDELPGKNDGMQVSSSRGNEIREEKLPKECPGCHFMKP
                     AGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYTKSDKQAWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEITPEVSNYIRHKFIRFARGRSRAEKMT
                     EKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQLQKNRNSLSQ"
     gene            complement(4567..4935)
                     /locus_tag="ACHKA1_005192"
     CDS             complement(4567..4935)
                     /locus_tag="ACHKA1_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682719.1"
                     /translation="MRAELKQVLINLLEEEFGHGMDKVTFDYVLQKKIKSLGCELQRC
                     FTVRLKGDRRGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIRALPEGISGFVFLRDG
                     KHPQRYIADGVDVIRATRFK"
BASE COUNT         1200 a         1398 c         1106 g         1353 t
ORIGIN      
        1 ggcagcataa aatcgtcctt ttatgaaaat caacgttttt tgagcactcg cacacaaaaa
       61 attaccgcac gggttgcaac gacaacctgc attttgcgga gcatatgaca actaaagtac
      121 gggtgcgttg aggatgcctg acacatcaga gggggcggga gattactccc ccgccaggtc
      181 tcttacttct cagattcgta gtctacgaag acagcgacct ccgtctggcc ggttcggatt
      241 cgtacatcgc agaggtcttt cctcgctacc agtaccgtca gaatgacggt taaacagatg
      301 acgatcaggg cgattaacat cgccttttgc tgcttcatag cctgcttctc cttgcctttc
      361 ggcacgtaag aggctaacct acatgtgttc agcatgaaat tggcctcaga ttaatgttaa
      421 gcgtcttgca ggacgcgtaa tgttaactgg ggcttttctc cgtctgcctt acggcagcat
      481 gcccgaggca gacaccctca agcacccgca gtcattctac agtaaaaccc cgcgctttca
      541 atttcccgta tgtccggaaa actgactgac ctgtgcccct gatcgttcac gctcaacgta
      601 tctgtccatt tccagtttta ttccctgcat aatcaacatc ccctcaataa caccttcagc
      661 tttttgcagt acccgcccgg cccagcaatc agagcgctca tgcttacggc ccagtcccat
      721 aagcgtcatc ccgaacacat aataatcata cagaaaatca tgcaaatcgc tgttgttgac
      781 gttcagttgc gccatgcaat tgctgataat cagcgcatca tcatcggtac actgaggccg
      841 tgatttaacc tttgatggga tcactccctt aaatccggca gcgacgccgg accatgccac
      901 actctcatga ttatttacca cccatgcacc ccatcgggac agaacctgtt ggatatcacg
      961 cattattctg tactccgctg taaccatact ttttctgatt tgaatgctca gggggaaaat
     1021 gtctactgga tatctccggc ttcattgctc acactcccca accagattga gaatgaccgc
     1081 tgaaccattg tcttccatgt actcatcttt tccgcttgcc agaaaccagc gacacacttc
     1141 aaccgcttca tcacgcgtca caggctttat ttttcccagc aaattttcga gccagaactg
     1201 ccgatcatat actgactcat gatgttcacc tgtgaaaccg tatttgtccc ctgtttctct
     1261 ccccgctgtc tggcgcatct cataaagcca gtcccagtaa ataaactcac gaacaacgtc
     1321 tgacaatgta tgaggctctg gcagtacatc acgatagcca tcaacatatg cacgacgctg
     1381 atcatcaatt tcatttgagc gaacaccgtc aatacttccg gcttttttct ctgccgcagt
     1441 ccagccccag tagtaatcgt cgataaattt cggggaggtg cgaattatcc gctcagcctc
     1501 cacatcatcg agtgctgcct catagctgcc aaatgtggcc ctgactgatg cggctttttt
     1561 gatattctca cgggcattcc tgatcgcctg cgccgggtta tctatgccta tggttgcgaa
     1621 agcaatctgg aaaggatcaa cattaatctc ctgcaaataa cgtccatacc gttcactcgc
     1681 ttcttccggc gttattttga gtttctccag tgctgcttcg gctgcgtcca gatgtacggc
     1741 ctcgttcagg tgaataacct ccagaatcca gagataagca tctgtctgtt tatgtccggt
     1801 gatcctcctt tgttctggca gaggtttgat atttgccgga acggcgctgt acgctgccgt
     1861 cgggatagtg aataaagctt tatgtacctg attgtccgta cgcatcacgc aaccgccttt
     1921 tttctgtaaa aaaccagttc ccgaacctga tcgccgttca tgagcatatt gttgaaatca
     1981 tcatgatcag gccaccggac acttacccgc tgaatgtcat ttcgggcctg gagatttgca
     2041 tgtgcgcatt cacaggccgc agccattccg gtagcactgt attcatcgcg atcggcaaaa
     2101 ataatcagat gggtgactcc cgccggtacg cggaactttt tcataaaacc actgttaatg
     2161 gttgcccagg tatttacttt gtaaatctga tgtgcggaca acgcggtttc gataccttca
     2221 gctataccca gtgtactggc taccggaaac atcctgatag caactgaccg ggcatggtcc
     2281 agatagttat cgtcctgtaa agatttgagc cttctggcgc tgataccaat gtctgctttt
     2341 ttagctccat caagcaatgt ctgatgcagg taacacagtt ctcccttgtc gtcggtcgcc
     2401 agggcataaa gagactggaa catgcgcccg ttataatttt gctggctgtt gaagcgaact
     2461 ccctcaaccg gaagactgaa tatgccacgg gaacgaagat attcagcacc agatgttccc
     2521 tgtagcgacg tgagcgctga aaatcgacgc tgaatgcgcg atctcaggct ttccgacgta
     2581 cagatcacag gcggtttatc tctccggtat tcattccccg tgatgcggtc tatttcagcg
     2641 caaatttcat gaaacgctct tttttgtgtc agagcgatca gtttcatgcc gtcaccgcta
     2701 ccacaaacac agatccacgt tccggtgcca tcacggtcat caatacggaa cttacctctg
     2761 gcaccacaaa ccggacattc ccctttgaag tgctttttac ccgtgactgg cggcagtccg
     2821 taatgtttta aaatttcagg ccagtggcct ttcgctgctt ctgctgtttt catcttactg
     2881 actcagactg tttctgtttt tctgaagttg ttgctttgcc tgcatgatct gccacgactc
     2941 actgccttcc ggcaccccag accggacacc atgaagcagg gacagagaaa gcgcttcggg
     3001 ggatttttcc gtcatcttct ctgcccggct acgccctctg gcaaaccgga tgaatttgtg
     3061 tctgatgtaa ttactgacct ccggggtgat ttccatcgga aaactgctca gcccgtcagg
     3121 ccattcgccg aacttctccc ggaaagtgtg agcgcaccat ccatcgctga ccggtcgccc
     3181 cagagaggca cgctgacgct gatagaattt gatctgactc caccaggcct gtttgtcgct
     3241 tttggtgtaa attttttcgt ccttgctgag tttcctgata ttgcggcggg tatctgtgtt
     3301 cacatcctca ccagccagcg gtttaaatcc gcatttcgga cagacataaa cacctgccgg
     3361 tttcatgaaa tgacaccccg gacattcttt cggcagtttt tcttcccgaa tttcgttacc
     3421 acgtgatgat gacacctgca tcccgtcgtt tttgccgggc agttcgtcat actcaatatt
     3481 ttcaggaaat cccaggcgat gaaccgtacc ggaatggtcg aaaatcaggc atgactcctt
     3541 gcctggcgct gtacgcaatc ctctcccaat cgcctgaagc cagcgaatct ctgattttgt
     3601 cgggcgtgcg taaatgatgc accggacatc actgtcaaat cccgccacca gaacgccaac
     3661 gctgacaatg attttcgtcg ctcccgtttc aaaacgactg atcattaact gacgctcatc
     3721 atgtggcgtt tcagcaacca tcacctctgc gttaattccg gctttgttga actgcatggt
     3781 cacgaagctg gcatgcgttt tattgacgca gaacgccact gtcggtaaat cccttccgtt
     3841 acacagccag ttatcgacaa tatcgccaac cagatccgcg ccgcacataa tctctgccag
     3901 ttgtgattcg tcgtaatccc tgccgtactc cattgagact ttcgttttta caccttccag
     3961 atccggtttc gtgggcgcat aaaattcgta attgctcagt tcgccacgct ggatcagttc
     4021 ccctatagtg gtcggcttga gaagacgctg gtaataacgc cccaggaacg atgaaaaggg
     4081 tgtaccggac aacccaatca ccttcacatt tccctctgca accagtcgtt cgatttccgt
     4141 gaggatgcgg cgcttacgga gatgagcttc atcaataatc agcaggtcga tattctgagg
     4201 aaaatcacgg cgaatcagcg tatccgcact ggcaatctga atcagtcgtg acgggtcgtt
     4261 attgggatga tcacgccaga taaagctgat ttcatccccg gaaagcccgt actgaacaaa
     4321 gcgctgtgct gtctggttga tcaaaatggt ataaggaacg acaaacagaa cgcgcatgcc
     4381 acggcttacc agaccagcag tgatgaacgc cgccaggccg gttttaccac tccctgtagg
     4441 catgtacatc atgaaggact gatatgtttt ccagtcatga cgcaacatat tcagcgcacg
     4501 ttcctgagca aaattcggct tgatattcag catggtattc tcccgatgac agggctgata
     4561 cagtgattac ttaaaccggg tcgcccggat gacatccaca ccatcggcga tataacgctg
     4621 tggatgtttg ccatcacgga gaaaaacaaa tcccgaaatg ccttcaggaa gcgctcgaat
     4681 tttcatcagt gaacgctgac ggggcgatcg gttatccacc tcaactgcgc aacgctgacc
     4741 atcgggagaa ataaccagaa gatcgataaa tcccctgcga tcacctttca gcctgacagt
     4801 gaaacagcgt tgcaactcac agcccagaga tttgattttc ttttgcagaa cgtagtcgaa
     4861 tgtgaccttg tccattccat gtccgaattc ctcttccaga aggttgatta gtacctgttt
     4921 cagttcagct ctcatatttt aaccatgatg tactagtttg ctgatacgtc cgttttttca
     4981 gggatgcccc tttagatcga gatctaccta acctctgtac ccgtctgttg gaagatcccg
     5041 ttccagtgct tcgcact
//
LOCUS       DAZFKF010000209         4987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFKF010000209 DAZFKF010000000
VERSION     DAZFKF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.211"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            110..826
                     /gene="nanC"
                     /locus_tag="ACHKA1_005193"
     CDS             110..826
                     /gene="nanC"
                     /locus_tag="ACHKA1_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO5682720.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            846..1952
                     /gene="nanM"
                     /locus_tag="ACHKA1_005194"
     CDS             846..1952
                     /gene="nanM"
                     /locus_tag="ACHKA1_005194"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO5682721.1"
                     /translation="MNKTITALAIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLETQAKDKKWTALASFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRDNYQNGKNYAHEGLKKSYSADIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2017..2997
                     /locus_tag="ACHKA1_005195"
     CDS             2017..2997
                     /locus_tag="ACHKA1_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO5682722.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDGEDALHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFIAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALTKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWRER"
     gene            complement(3005..3655)
                     /locus_tag="ACHKA1_005196"
     CDS             complement(3005..3655)
                     /locus_tag="ACHKA1_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5682723.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIDQGFHIHQDKAAAAPIADVLFPD
                     ELPSQGTYVEGAAIKVTVNSYERDKKAREKAVEYHGCQCNVCGVDLVKIYGDIAEGFI
                     HMHHLVPLSAIKEDYLLDPVNDLLPVCPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            3792..4664
                     /locus_tag="ACHKA1_005197"
     CDS             3792..4664
                     /locus_tag="ACHKA1_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFO5682724.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIVLPQVRKNYPY
                     EGFIEWHRKEVFRG"
     gene            4709..4888
                     /locus_tag="ACHKA1_005198"
                     /pseudo
     CDS             4709..4888
                     /locus_tag="ACHKA1_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT         1426 a         1097 c         1180 g         1284 t
ORIGIN      
        1 cggttgccaa agacactata agcctggcaa aaaatattac acaacataaa tgctaattgt
       61 ttatgcgggc tttgtattgc tttctgtatc ctacaaatga gtgaaattta tgaaaaaggc
      121 taaaatactt tctggcgtat tattactgtg cttttcgtca ccattaattt ctcaggctgc
      181 gacacttgac gtacgtggtg gatatcgtag tggaagccac gcctatgaga ctcgactcaa
      241 agtcagtgag ggatggcaaa atggatggtg ggcaagcatg gaaagtaata cctggaatac
      301 cattcatgat aataaaaagg aaaatgccgc actcaatgat gttcaggttg aagttaatta
      361 cgcgattaaa cttgatgatc aatggacggt gcgcccggga atgttaacgc attttagcag
      421 caacggcaca cgctacggac cctacgtaaa actgtcctgg gacgcgacaa aagatcttaa
      481 ttttggcatt cgctatcgtt acgactggaa agcttaccga caacaagact tatccggtga
      541 tatgtctcgt gataacgttc atcgttggga tggatatgtc acttaccata ttaatagtga
      601 tttcaccttc gcatggcaaa cgacgctata cagcaaacag aacgattatc gctatgcaaa
      661 ccataagaaa tgggcgacgg aaaatgcatt tgttctacaa taccatatga cgcccgatat
      721 tacgccatac atagaatatg actacctgga ccgtcagggt gtttacaacg gcagagataa
      781 tttatcggaa aacagttatc gcattggtgt gtcatttaaa ctgtagtaga caggagacag
      841 tcacaatgaa taaaacaata acggcgcttg ctatcataat ggcttcattt gccgcaaacg
      901 cgtctgtatt accggaaact cctgtgccat ttaaaagtgg taccggagca attgataacg
      961 acactgtcta cattggttta ggtagcgcag gtacggcatg gtacaagctg gagacacagg
     1021 ccaaagataa aaaatggaca gcgttagctt cattccctgg cggaccaaga gatcaagcaa
     1081 cctcggcatt tattgatggc aatctgtatg tgtttggcgg cattggcaaa aacagcgagg
     1141 gcttgactca ggtatttaat gacgtacaca aatacaaccc caaaaccaat agctgggtta
     1201 aattgatgtc gcacgcaccg atgggcatgg cgggtcatgt gacttttgta cacaacggca
     1261 aggcttatgt tactggcggt gttaaccaga atatcttcaa tggctatttt gaagatctca
     1321 acgaggctgg aaaagattca accgctatag ataaaatcaa cgctcactat tttgacaaaa
     1381 aagcagaaga ttatttcttc aataagtttc tgttgtcttt tgatccctca acccagcaat
     1441 ggagttacgc tggcgaatcg ccctggtacg gaacggctgg tgcggcggtt gtgaataaag
     1501 gtgataaaac ctggcttatt aatggtgaag ccaaaccagg attgcgaacg gatgccgtat
     1561 ttgaacttga tttcaccggt aataatttaa aatggaatag gcttgctccc gtctcatcac
     1621 cagatggcgt cgctggcggt tttgcgggga taagcaatga ttctcttata tttgccggag
     1681 gggccggatt caaaggttca cgagataatt accagaacgg taagaactat gcgcatgaag
     1741 ggctgaaaaa atcatatagc gctgatattc atctttggca taacgggaaa tgggataaat
     1801 cgggtgaatt atcgcaaggt cgggcctacg gagtatcatt gccctggaat aatagtctat
     1861 tgattattgg cggtgaaact gcaggcggca aagcggtgac ggattcagtt ttgatctctg
     1921 tgaaggataa caaagttact gtgcaaaact aacgcttcag ggccccagta aaggggccct
     1981 gctatcaatt tgtcatttca attacgcgaa aattatatga acgcaataat atcgccggat
     2041 tattactatg ttcttaccgt tgctggtcag tctaatgcca tggcgtatgg cgaaggactg
     2101 ccattaccgg acggggaaga tgcgcttcat cccagaatta aacaattagc gagatttgcg
     2161 catacgcatc ccggaggccc gtcatgtcac tttaacgaca ttattccact gactcactgc
     2221 ccacacgatg ttcaggatat gcagggttat caccatcctc tggcaacgaa tcatcaaaca
     2281 cagtacggca ccgttggcca ggcactgcat attgcacgga aattactgcc cttcattcct
     2341 gataatgcag gggttctcat cgttccatgt tgtcgtggcg gctcggcttt tatcgcaggc
     2401 agcgaaggga catattcaga acggcacgga gccagccatg atgcttgtcg ttggggaacg
     2461 gatactccgc tataccagga tttagtcagc agaacgcgag ccgcactgac aaaaaatccg
     2521 cagaacaaat tcctcggcgt atgctggatg caaggcgaat ttgacttaat gaccagtgac
     2581 tacgcgtcac accctcaaca ctttaatcat atggttgaag cctttcgtag ggatctaaaa
     2641 caataccatt ctcagcttaa taatattact gacgcgccgt ggttttgcgg cgataccacc
     2701 tggtactgga aagaaaattt ccctcatgcg tatgaagcta tttatggcaa ttatcaaaat
     2761 aatgttttag ccaatattat tttcgtcgac ttccagcaac aaggtgaaag aggactgacg
     2821 aacgcgcctg atgaagatcc ggacgattta agcacgggat attacggttc agcgtaccgc
     2881 tcaccggaga actggacgac ggcactgcga agcagtcatt tcagcgcggc agcccgtcgg
     2941 gggattattt ctgacaggtt tgtagaagca attttgcagt tttggcgcga aaggtgagcg
     3001 ttcattaatt tgatttattc gcatccatca acgcttttaa ctcctccggc gtaaacggtg
     3061 gtttgcgtcg gtgtaacatc gcatggcaat tagggcacac aggaagtaaa tcattaactg
     3121 gatccagcag gtaatcctct ttaatggctg aaagcggcac caggtgatgc atatggataa
     3181 agccctcagc gatatcgcca tagatcttca ccagatcaac gccgcatacg ttgcactggc
     3241 atccgtgata ttcgactgct ttctcgcgag cttttttatc gcgttcatag ctgtttaccg
     3301 tcacctttat cgccgcacct tcaacgtagg tcccctgcga cggcaactca tccgggaaca
     3361 gaacatcggc tatgggagcc gcagcggctt tatcctgatg aatatgaaat ccctgatcta
     3421 taagtagctt gacgcattta gattttattc cgccggtgaa atccgcaggg gtaaattgcg
     3481 tccccgtcat caatgtcgct gcgatgccag caatcgcctt cgaggggtaa cgtcggccat
     3541 gatattcaat ctcatacaac cgtgcaggct ggaactgatg agtttctccc gcatcccata
     3601 aatgaatagc ttcgataata taccgccgcg gaagatgatc ggggagttta ttcatagaga
     3661 atgtccttat acgttctcag aacaagcagc aaaaaaacaa atgagatcaa agtaatatat
     3721 cttcacttgc aggcttgaac agcagaaaat ttgcgagcat gatcgcaatt atttcactgc
     3781 cagggatatc aatggcttcc agtaaatcgc tacagcaggc aattgccaac ataaaaatct
     3841 ggcacaaggg tgaacagcgc gcgccgcata aaccattgtt actgctatac gtattagcgg
     3901 gatacctgaa tggacatcca cgccttttcg attatggatc ggaaatctac gaacctctgc
     3961 acagtttact ggaacgtttt gggccacagc gttcacagta ccgacctgac atgccgttct
     4021 ggcgattaca aggcgacgga ttctggcaat tacacaatgc cgagctttgc tcaacagcag
     4081 ggagcagccg acaaccgcca gtgaaggaac tgaacgaata ccacgttgca ggcggtttcg
     4141 atgaacagca ctacgccctg gtaaccggca ataaaaaact catcaatact ctggcgcaac
     4201 agatactcga agcgcacttc accgagagta ttcaggaaga aatcgctgac gagctgggtt
     4261 ttgatctcca gcaaatccgt aaacagcgcg acccgttatt tcgcaaaaat gtattacgcg
     4321 cttataacta ccagtgtgcg atatgtggct ttaatatgcg ccacgacgac accaccgtcg
     4381 cgctggaagc ggcgcatata aaatggaaac agcacggcgg tccttgtgaa attcctaatg
     4441 gcctggcgct ttgcgcaata caccataaag cctttgataa aggatcgatt ggcctggacg
     4501 aaaatatgcg ggtactggta tcagatgcgg taaacggcgg agggatcgtt gagagattat
     4561 tctgggattt tgacgggaaa actattgtgt tgccccaggt gcgtaaaaat tacccttatg
     4621 aggggtttat agagtggcat cggaaagagg tttttagggg gtgaggttga taactatcgc
     4681 agtatgttca atattgctga cataatcagt gcaggatctt gccgctagtt tacaattcgc
     4741 cttcgatagc ggcgcaaaac gagttctgtt gccgatatcc tcggctatgg atattccaac
     4801 agttccggca gagttgttta ccaagtttca ggtaagtttt tactcagaac cggttgatgc
     4861 ggtttataag gcgctggatg tgaattaaca cattcctcag cttatttata aaaggattat
     4921 tcccatttga tataatatca gtgccaattt acttttttca atggaatata tctcggctaa
     4981 ataacaa
//
LOCUS       DAZFKF010000210         4807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFKF010000210 DAZFKF010000000
VERSION     DAZFKF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.212"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..473
                     /locus_tag="ACHKA1_005199"
     CDS             <1..473
                     /locus_tag="ACHKA1_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFO5682725.1"
                     /translation="SSVGSRMGLGRFSGFFRSAGGLAGRLGGGALWAGAMAAPVLLDG
                     SASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNYLGGWLTEAWQKLR
                     GSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNVWNGGNYGLW"
     gene            460..1809
                     /locus_tag="ACHKA1_005200"
     CDS             460..1809
                     /locus_tag="ACHKA1_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFO5682726.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLTEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGQVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAVSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG"
     gene            1793..2917
                     /locus_tag="ACHKA1_005201"
     CDS             1793..2917
                     /locus_tag="ACHKA1_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO5682727.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            2907..3521
                     /locus_tag="ACHKA1_005202"
     CDS             2907..3521
                     /locus_tag="ACHKA1_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO5682728.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNITVDKNLHVKGNV
                     SIDGTGRSQGAFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            3514..3951
                     /locus_tag="ACHKA1_005203"
     CDS             3514..3951
                     /locus_tag="ACHKA1_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO5682729.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPIGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHDRLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            3951..>4807
                     /locus_tag="ACHKA1_005204"
     CDS             3951..>4807
                     /locus_tag="ACHKA1_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO5682730.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFE"
BASE COUNT         1000 a         1255 c         1532 g         1020 t
ORIGIN      
        1 tttcgtctgt cggtagccgc atggggctgg ggcgtttctc cggctttttc cgcagtgccg
       61 gtggactggc cggccggctg ggtggaggtg ccctgtgggc tggcgcgatg gcagccccgg
      121 ttctgctgga tggcagcgcc agtgccacag acaagggaga agcggtcggc tctcttgcag
      181 gcagtattgc cggtggtgcg ctgggggcgg cagccggacc tgtggggatt gccattggtt
      241 ctacggtggg cagctatctc ggtaactatc tggggggatg gctgactgaa gcctggcaga
      301 aattacgggg cagtgatgat gaaagcagtg gacaggcggt acaaaaagcc tctgcccgtg
      361 tggagctggt tgccccggaa gggtggcagg ctcgcagtat tgatattgat gacacgtccg
      421 gtcacggact ggatgtgaat gtctggaacg gagggaatta tggcctctgg tgacggtcgt
      481 ggtgcgttcc gtggtgtgcc gttcctcgtc tggcgtgagc agcgcgaacg tggtgggcga
      541 aacattgtcc gtcgggaata tccgttacgg gaaaccggtg gggcagacga tctgggacct
      601 aaactgactg aatttacgtt cagtgtactt gtgatggggg atgatgttca gacacagcga
      661 aatcgtctgc gtgatgccct tcgtgctccc ggtgccgggg agctgcttca cccggattac
      721 ggcacgttaa atgtgctgat aaacagcttt gaaagtcgtt ataacgccgc tgaacagggc
      781 gtggttgagt tcaccattaa tgtcacgccg gtaagtgatg ataccgcacc tgctgtcaca
      841 caggacacgg ccgccattct ggagcagaag agtaccaccg cgctcgggca ggtttttgaa
      901 accctggaag cgggctggac ggtgatctct gacggaatgc acgatgtcca ggcaatgacc
      961 gaaaccatca gtgataaggt gtccgccctg gaaaatgccg tttccggcat ggggattgta
     1021 caggatatca gtgcctttac tgccacgttc acggcgctga aagggaatgc cacggcatta
     1081 ctgacagcac cttcccgcat ggcctcatct tttgcggggc ttttcagtgc cctgatcacc
     1141 ctgccatcgc tgccgtcact gtcttcagga ggcctgaata cccggcccgg tggcagtatc
     1201 ggccggacga catccgccgt ctctcaggga atgccgcaac tgtacaggac attatcttcc
     1261 ctgcgttacg tgctggatga gcaggataat ccgcagactc tcatcggtct gacaccggca
     1321 gcacaaaaga atattcgtct gatacgggcg gtaatgcaga gtgctgccgt ggtggcccag
     1381 gcacagaccg tgggaaaact gctggatcag gttctcagtc aggaaaccct gccggacagt
     1441 gacgcagccc accgtacctg gcctgtctgg ctggaaagtt cagttgatct tcagcgcatt
     1501 aaccgtgact taagcgaagc gctcgaacgg caggtgatga cactgtccgg gcaggggtat
     1561 accgccacgg cgctgacgct tcgtgatgcc agactggcac tgacagagga tctgaataca
     1621 cggggagttc agctacccgg tgcaacagta gtgactgtac gtaccaccga gcctgcactg
     1681 gtgaccctgt accgtgccac cgggaacagt accggctggc aacgttttgt gcgccgtaac
     1741 ggtattgttg atccgctgtt cattcccgga ggccattcag tggaggtgat tagtgagcag
     1801 cagggttgaa ctgtatctgg gcggtgagat tttttccggc tggctgacgg tgagtgttcg
     1861 tcgctctctt gaacatctgg cgggctcctt tgaactgggg gtaatgatgc ccggtgtacg
     1921 ccttccgtca tccgtccgtg ccggtcagtc tctggaattg cgcattgacg gtcagcctgt
     1981 gatcactggc tggctggatc aggtccggca gcgcatcagc gccacgcgtt ttcagatcac
     2041 gctcagcgga cgggataaaa ccggtgacct ggtggactgt tcagccattc atccgggcag
     2101 ccagtggagg aaccgcacgc tggagcacat tgcttcagat ttgtgtgctc cgttcggggt
     2161 cacggtgcgc tggcaggtaa atgatgcaac ggcagcccgg cccttttcca ccttcacact
     2221 ggaaaattca gaaaccgtgg cagatgcgct gacacgggcc gcgagacaca gaggcgtcct
     2281 ggtaaccagt aatgccgccg gtgaactggt tttcactcag gccggcagtc agcgtggcga
     2341 cacgctgacg ctgggcgaaa atctgctgga tttggatcac aacgtggatc accgtctgcg
     2401 ccacagtgaa taccgtgtac gggggcacgg gcgtggtggt ggtcatgccg gggatgcact
     2461 gacagccgga acgctggccg caccagttgg tacggtgaca gacagtgcca tccaccgtta
     2521 cagaccgaaa attgtgctgg cggatcatgc tgttgatgca gacggtgcac gccagagggc
     2581 tgtccgggaa atgcgccggg cggttgcccg ctctgtgcgc ctgacagcca ccgtgcggca
     2641 ctggtttcgg gagaacggcc agttgtggga tatcaacctg ctgacggctg tcacggctcc
     2701 ccgcaccgga gtggaagagc gtgatcttct tgtctgtcag gtggagtttt cgctggatgc
     2761 aaatcacggc gaaaccaccc gtctgattct ggcaccccgt gacggcttta ttgttccggc
     2821 agagccggga aacagcggaa gcggaaatgc gggtgacgtg gacgccttcg tgcgggcaca
     2881 gatgaaaaaa caggggatta aattcaatga tgaatgacga agtcatcagc cgccttctgg
     2941 cccccgtgat gcggggtgtt cgtctgctgt tcgggcgtgg tgtactgacc ggcacaacgg
     3001 acacgctgaa aatccagaat gtgcaaatca ccggtatgga tggtgaaacc tttgatgacg
     3061 ttgaacgccc ccagcagtac gggcaaatca gcgtccccct gcctggtgcg gaagtttttc
     3121 tggcctgtgc tggcggacaa cgggatcagg ccgtggtgct tgtggtggaa gaccgccgca
     3181 gtcgcccgac cggacttacc gccggagata ccggcgtgta tcaccatgag gggcaccgta
     3241 tacgtctgac aaagaacggc cggatcattg tgacctgtaa gacactggag atttacgccg
     3301 atgaaggtgt tcaggtggat acaccggagg ctcactttac cggtaatatc acagtggata
     3361 agaacctgca tgtcaaaggc aatgtgtcca ttgacggcac cggaagatca cagggggcgt
     3421 tcacgatgtc cgaagcggtt attgccggga tcacctattc aggtcatgtg catcacgata
     3481 acggtgaagg cagcaaaacg ggaggaccag agaatggctg atattgctgt cgtctgggat
     3541 cagggttgtg gttccctgca actgaacggc gcagatcttc tgacggataa cagcctgctg
     3601 actgcggtca ttatttcact gtttacggac aggcgggcgc tggattccga tgaaatccct
     3661 gacggcaccc gtgaccgtcg gggatggtgg ggagacagtt tccgggagcg ccccattggc
     3721 tcccgtctct ggctgttaag ccgtgaaaag acgctgtcct ccgtggtcag ccgtgcacag
     3781 gcctatgctg atgaagcgct ggcgtggctg cataaaagcg gtgctgccac atccgtggta
     3841 tgtcatgcca tgcgtgtggg gcatgatcgc ctttcgcttt ccgtgaaaat caccctgccg
     3901 gacggaagca gacatccgat gattttttat gctgatatga agggggaatg atgccttatc
     3961 agcccttacc actggcgcaa ctgatcacac agacacaaca ggatatcagc cagcgcctgc
     4021 ccggttcgca gccgggcgtg aatgaaacca ccctgaatgc cattgcgtat gcactggcgg
     4081 gtctgtcagc acaggagcat gaacatctgg cctggatctc ccggcagata attccgacag
     4141 aagctgatga agccgaactc ctgaaacact gcgcattctg gggtgtcatc cgtaaaccgg
     4201 cttcccgcgc tgacggaccg gtacaactga tgctgaccac ggatgcaggg atcacggaag
     4261 gcgtactcct tcagcgaagc gatggtgttg tgtaccgcat caccggctct gcgaccggaa
     4321 aagccggaac actgaatgtt aatgtggagg cggaaagtgc ggggcgcgcc ggaaataccc
     4381 cgaccggaac ccgcctgtcc tttatcacgc cacaggcggg catcaaccag acagccacgg
     4441 ttaccggcac gggactcacc ggtggtgcgg atgtggaaac ggtgccggag ttgctgtcca
     4501 ggctggtatt ccgggtacag aacccgccat cagggggaac acagtatgat tttgaacgct
     4561 gggcacggga agtaccgggc gtgacgcggg catggtgtaa gcctgaatgg cctgaggcgg
     4621 gtagtgttgg tgtgactttt gttcaggata ataaccctga cattttcccc ggagaaggtg
     4681 atgtgaagcg ggtggcggat tatatccgca gtcatgatga tccggcgacg ggccagcccg
     4741 ttggtcagcc acttgggccg acaatcagcg tgtttaagct gaccaataag ccggtggcgt
     4801 ttgagat
//
LOCUS       DAZFKF010000211         4783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFKF010000211 DAZFKF010000000
VERSION     DAZFKF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.213"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(90..1787)
                     /locus_tag="ACHKA1_005205"
     CDS             complement(90..1787)
                     /locus_tag="ACHKA1_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFO5682731.1"
                     /translation="MEHQLNKKLLNSSISLILASGLMMSTANAADDVKLKATKTNVAF
                     ADFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAI
                     EAAKKSTPTLAALMDEGVKFTNGYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGI
                     PLEETFLSELLQNHGYYTAAIGKWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQ
                     NRGFNYFMGYHASGVSLYNSPSLFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPF
                     MIYLAYSAPHLPNDQPAPKNYQEKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYDKL
                     VSSMDFYPTALDAADIKIPASLKLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSH
                     WWDEENTAFWNGYHKFVRKESDDYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLAL
                     YDINDLQQTKDLSASHPDVVKNMKAVMIDFITSSQKPISDINQAKFENIKKSLTNVK"
     gene            complement(1883..3019)
                     /locus_tag="ACHKA1_005206"
     CDS             complement(1883..3019)
                     /locus_tag="ACHKA1_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO5682732.1"
                     /translation="MRKLSMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFTKKAGVATDNILAVGMMYQF"
     gene            3695..3903
                     /locus_tag="ACHKA1_005207"
                     /pseudo
     CDS             3695..3903
                     /locus_tag="ACHKA1_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            3890..4079
                     /gene="tnpB"
                     /locus_tag="ACHKA1_005208"
                     /pseudo
     CDS             3890..4079
                     /gene="tnpB"
                     /locus_tag="ACHKA1_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(4082..4270)
                     /locus_tag="ACHKA1_005209"
     CDS             complement(4082..4270)
                     /locus_tag="ACHKA1_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO5682733.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
BASE COUNT         1309 a         1063 c          948 g         1463 t
ORIGIN      
        1 tttattttcg tgtttgttca ttatttattg ctctaactga tttatttccc tatatccagg
       61 gagtagatat atctactccc tgcttattat catttcacgt tagttaaaga tttttttata
      121 ttctcaaatt tggcttggtt gatatcactg atcggttttt gacttgacgt gatgaagtca
      181 atcataacag ccttcatatt cttaactaca tcaggatggg atgcagagag atctttagtt
      241 tgttgcagat cgttgatatc gtacagagct agtttattct ctgcataggt gtaaaccaga
      301 gaataatcgt tgcttcttac agtgtatgca aactggctta agtcttctgt atgtgggttg
      361 tgcggataat catcagactc ttttctcacg aatttatgat atccgttcca gaatgcagtg
      421 ttctcttcgt cccaccagtg agaataagag gtaatccagg acagatattt gtgaggttcg
      481 ccagctgcag atttcttatc agctcctttc tgtacatatg gcaataatga tacaccatcc
      541 agtttcagag aagcagggat cttgatatct gcggcatcta aagcggtcgg atagaagtcc
      601 attgatgaaa ccagtttgtc ataatcaccc gttttcagct tgccattcca ccacatgaac
      661 ataggtgtat gcataccacc cggatacggt tggcctttgt aaccagcctg cgcaccattc
      721 agtggcagtg gaccatcaat tacagcaccg ttatcagagg tgaacagaat gatggtgttg
      781 tcgtactgac ctttttcctt aagtgtattc aggatctttt taaccccctg gtcaacggag
      841 taaattgacg cataatagtt atcagcagtc ttgctaccag tgttgaattt ctcctgatag
      901 tttttaggcg caggctgatc gttaggcagg tgtggtgcac tgtatgccag ataaatcata
      961 aatggctgtt ccagtttgct tgcacggtca attacaccaa ttgcttcatc agttaattga
     1021 tcactaatgt acccttcagc tttaatgttt tcacggttac ggaacaatga cggggagtta
     1081 taaagtgaaa cgccggatgc gtgatagccc atgaagtagt tgaagccacg attctgaggt
     1141 tgccattctt ctgcggagaa ggtggtgaag ttatcgtggt agtcacgggt ttgtttttct
     1201 tcaggcacac ctacgttgct gatttttgat aagtgccact taccgattgc tgcagtgtaa
     1261 taaccatgat tctgaagtaa ttcagacagg aatgtttctt ccagagggat accatcctgt
     1321 gcatcagaat ttgaatatac accgaagcga gcaggagctc gcgccgtcag gattgcggca
     1381 cgtgaagggc cagagacacc gtgtgcaaca tagccgttag tgaattttac accttcgtcc
     1441 atcagggccg ctaatgttgg agtggatttt tttgcagctt caattgcttt atcaatacct
     1501 attttgtatg tatcaacaac atttcggtta tccatcgttt tagggtcaaa tgaactatca
     1561 tcaaaaggca attgtccata ccctaaatca tccattgtta aaacaatgat atttggctta
     1621 cctttagtgc tgtattccgt cggcgtgaag tccgcaaagg caacattggt ttttgttgct
     1681 ttgagcttaa catcatctgc tgcatttgca gtactcatca tcagaccaga tgctaatata
     1741 agtgatattg atgagttaag cagtttttta tttaactgat gttccatatg gtattccttc
     1801 tgagttgcat cacgaacatt tattaataat aaaagcctct gatataaata aaaactatgt
     1861 aaatcgttta aatggatacc tgttaaaact gatacatcat accaacagca agtatattat
     1921 cggttgcaac tccagccttt ttggtaaatt cgctttcatc aataaggtta attttgtaat
     1981 caataagaag tgacatgttt ttgttcagaa agtagtttgc gccaacatca atatactcaa
     2041 caagagactg atcgccatag tttactattc cacctttaac atccatccct ttggactgaa
     2101 cgtaagccag tgaaggacgc aggccgaaat caaacatata ctgagccaca acttcaatat
     2161 tccgtgcttt atctgcgatg cctgcgctac caaatggagt catatttcgg gtttcagagt
     2221 agtcagctgc cagatacagg tcattatcat tatacttgac cccaacacct gcaaactccg
     2281 catgttttcc cccggcaatc cagcgaggcg taataacttc ttttccttct gaatctttcg
     2341 atgtcatgaa acgtttacgg ccttcttttt cctgcagact ggttctgtcg gcattagtgt
     2401 aagcggcaac gattccaaaa ccttcatagt catagcttac ggagaaacca tatccatcac
     2461 cattagcttc aagtaaatta tcacggtcat ttttaccctg atattgagca gaaaaagtaa
     2521 gtccatcaac aagcccgaag aaatctgtat tgcgccaggt cagaactccg gttgttctgc
     2581 cggtcatata gttatcagag taagtccagc tgtcaccacc aaagtccgga agtacgtcag
     2641 tcagagcccc cacatcatag gcaacaccat agtttcgccc ataatcaagt gaaccgagta
     2701 tattgtggcg aatacctgca aatgccagac gatttttatc gttgcgtact tcactttctg
     2761 tcttaaaccc caggaactgg tattcccact gaccaaagcc agtcaggctc tcattaattt
     2821 gtgtttcgcc tttgaaacca agacgtgcaa atgttctgtc gccatccata gaaccatcag
     2881 agatgtaatg acgggcttta acgcgaccat aaaaatccag tttattaccg tctttattat
     2941 agatttctgc tgcctgtgtt gcctcacaca gcattaatgc agaaacgaca gagcacacaa
     3001 ccatacttaa ttttcgcata aaaaaatccc ctgtgcacat ttttagtatg gttttaaaaa
     3061 ttaaactatg gcgaaaaaat tatcactatg caaagatgta acaaaatatt gctcagtgat
     3121 ctatgttgtt gtttttattt gaggttgtat attttctagt gtgtttgact tttctttctc
     3181 aagtgttatt tcttgacctt tatcacagaa ccatctccct ggatatcccc atcacattgc
     3241 atgaaaagca agttttacca atcttgattt tcagtgttaa ataagattaa taactgtaat
     3301 tctgcaaaaa aaagaacacc atatgtagta tgactacgta ttccactctg ttcgttcacg
     3361 gactggcatc gaagtctgag gtgtactggc aatagcggat acagtcattt gttctttttt
     3421 taagcattca tccgatgata cttttcctga atgctaccgg agtggtattc ccctgccgtg
     3481 agggagggcg ctgacggtta taaaaatcct gatatatacc cattcccgta tgacagcgat
     3541 ggcgtcacat ctctgttcta ccatcttcac agaaatattt cgttcattag cattccggta
     3601 ttcgtataac caccttcggt ggagaaagaa taaacaatcc ctggaatgcg atgagcgctt
     3661 gaccagaaaa gcatcatatt gcttcatggc gcatcagcga ccacaggatg ccgcaaaagc
     3721 cgaaaaacgg accagagcgc ctgcaatcat tcctcgcctc agtatctgtt gcccatggag
     3781 tggccgataa cagatcattg tcggatgagc cttatcccct tccttcccgg tggtatccgg
     3841 atgtgctgcc agccttctca gttaaccgat gttttccggg ctttttgcca tactgaggac
     3901 tgataacgta ttcctggtcg taaaacccgt caatatgcgc cggggcatcg acaaaactga
     3961 cacagtacgt ccggaaagaa cttaacgctg catggcacga caatgctgcc tttgtcttta
     4021 ccaacaaggc tcgttcccgc ataaaagttc tacgccggga taaacatggc gtatggctgc
     4081 gtcaggcctt cactacttca caccggaagt cttccaccct gacacgaata taccgtccgg
     4141 gctctttcgc aggtgcaaac agtttttcac attcggcgaa tgtcatttct gaatcctggt
     4201 aacgcaacca ctgaaccggc atattaccgg gtttgattat tgtcgcagtt attttatatc
     4261 gtttccccat atcgcctctc atcctgacaa caaaattacc ggctgagcca tcactccggt
     4321 aaagcatgcg aaaaaaagtg agcgtctcag caatggctca tgcgatgatt ctgtacgggc
     4381 accgatggca cctcagaata caacaaagat atcagagtaa catgtctctc tcgttatttt
     4441 tcagagttct tgacaccctc cttctgccac attgtcaacg ccagtggaga cttctgctgt
     4501 cagttttttc atccatacag agtgacatta attatcctga acaaaacgaa atatcctgaa
     4561 tatatttatt ttctcctggt gccggagaca tgatgatgta caggctcagg tgcggtgtga
     4621 atgtaaaatg gtatgttaat tattcatttc tgaatgcaaa ctgaatgcaa acttattccg
     4681 aattgaattc tcctgacacc acatgataaa aatgaggata taataaaata cttctcttct
     4741 ggatgtgcca ttacctgccg ttgctgtaat acggcgggta ttt
//
LOCUS       DAZFKF010000212         4745 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFKF010000212 DAZFKF010000000
VERSION     DAZFKF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.214"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..617)
                     /locus_tag="ACHKA1_005210"
     CDS             complement(<1..617)
                     /locus_tag="ACHKA1_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO5682734.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERA"
     gene            complement(732..807)
                     /locus_tag="ACHKA1_005211"
     tRNA            complement(732..807)
                     /locus_tag="ACHKA1_005211"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(772..774),aa:Thr,seq:ggt)
     gene            complement(814..888)
                     /locus_tag="ACHKA1_005212"
     tRNA            complement(814..888)
                     /locus_tag="ACHKA1_005212"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(853..855),aa:Gly,seq:tcc)
     gene            complement(1005..1089)
                     /locus_tag="ACHKA1_005213"
     tRNA            complement(1005..1089)
                     /locus_tag="ACHKA1_005213"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1053..1055),aa:Tyr,seq:gta)
     gene            complement(1098..1173)
                     /locus_tag="ACHKA1_005214"
     tRNA            complement(1098..1173)
                     /locus_tag="ACHKA1_005214"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1138..1140),aa:Thr,seq:tgt)
     gene            1535..2485
                     /gene="coaA"
                     /locus_tag="ACHKA1_005215"
     CDS             1535..2485
                     /gene="coaA"
                     /locus_tag="ACHKA1_005215"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO5682735.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2514..3479)
                     /gene="birA"
                     /locus_tag="ACHKA1_005216"
     CDS             complement(2514..3479)
                     /gene="birA"
                     /locus_tag="ACHKA1_005216"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO5682736.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3476..4504)
                     /gene="murB"
                     /locus_tag="ACHKA1_005217"
     CDS             complement(3476..4504)
                     /gene="murB"
                     /locus_tag="ACHKA1_005217"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO5682737.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGRVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4641..>4745)
                     /gene="rrf"
                     /locus_tag="ACHKA1_005218"
     rRNA            complement(4641..>4745)
                     /gene="rrf"
                     /locus_tag="ACHKA1_005218"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1187 a         1205 c         1091 g         1262 t
ORIGIN      
        1 gcacgctctg gttccggaat gtaagaatcc aggaagccag ccagttccag gattttcgct
       61 tcccactctg cgtcgccttc cagcgctttc agagcagaac cacgaacgat cggagtgtcg
      121 tcgcccggga agtcgtactg agacagaagt tcacgaactt ccatttcaac cagttccagc
      181 agctcttcgt catcaaccat gtcacatttg ttcaggaaca cgatgatgta cggaacgcct
      241 acctgacgac ccagcaggat gtgctcacga gtctgcggca tcgggccgtc agtcgcagca
      301 actaccagga tcgcgccgtc catctgagca gcaccggtga tcatgttttt aacatagtcg
      361 gcgtgccccg ggcagtctac gtgtgcgtag tgacgggtcg gggtgtcgta ttcaacgtga
      421 gaagtgttga tggtgatacc acgagctttt tcttccggcg cgttatcgat ctggtcgaat
      481 gcgcgagcag caccgccgta ggttttagcc agtacggtag taattgcagc ggtcagcgtt
      541 gttttaccat ggtcaacgtg gccgatagta ccgacgttaa cgtgcggttt tgtacgttca
      601 aacttttctt tagacatcga ttgtccctct aagacacgga taaatcggtg atatcaccac
      661 atcaaccagg caacatgccc gacttgttga atgcaataaa cagaaggaaa aaacagggag
      721 gagaaaagaa gtggtgctga taggcagatt cgaactgccg acctcaccct taccaagggt
      781 gcgctctacc aactgagcta tatcagcaca tcttggagcg ggcagcggga atcgaacccg
      841 catcatcagc ttggaaggct gaggtaatag ccattatacg atgcccgcat cctggaactc
      901 ggctacctga ttttcattct gcactgaata tcgagagaag ctctctttat tcgagccggt
      961 aagcgaactt atcgtctcgg gctacgccat cgcgtggccg aaattggtgg tgggggaagg
     1021 attcgaacct tcgaagtctg tgacggcaga tttacagtct gctccctttg gccgctcggg
     1081 aaccccaccg gacttgatgg tgccgactac cggaatcgaa ctggtgacct actgattaca
     1141 agtcagttgc tctacctact gagctaagtc ggcatcaagt agcgcgcatt ctatggagac
     1201 atgcgagttc atgcaactaa aaaattgcat aatttgtttt attggtcaca ttttatgcga
     1261 cacgatgaag aaacagccga atagagggca gattagcaca ctttttcaac atcattgtgc
     1321 tcaacaatgc gctcctgcta aaccataatt ctttttatca gatggaatat ctgtcacatt
     1381 gcttttcaac gatagcttcc tgggagagat tttttcttat tattcctccc catctggtgt
     1441 taccctcctg cccattaacc cattcaacag aactgtgacg cgccatggca aatatcgctt
     1501 tgccgataga gctatgaccg ccagaaacat gcttatgagt ataaaagagc aaacgttaat
     1561 gacgccttac ctacagtttg accgcaacca gtgggcagct ctgcgtgatt ccgtacctat
     1621 gacgttatcg gaagatgaga tcgcccgtct caaaggtatt aatgaagatc tctcgttaga
     1681 agaagttgcc gagatctatt tacctctgtc acgtttgctg aacttctata taagctcgaa
     1741 tctgcgccgt caggcagttc tggaacagtt tcttggtacc aacgggcaac gcattcctta
     1801 cattatcagt attgctggta gtgtcgcggt ggggaaaagt acaaccgccc gtgtattgca
     1861 ggcactatta agccgttggc cggaacatcg tcgtgttgaa ctgatcacta cagatggctt
     1921 ccttcaccct aatcaggttc tgaaagaacg tggtctgatg aagaagaaag gcttcccgga
     1981 atcgtatgat atgcatcgcc tggtgaagtt tgtttccgat ctcaagtccg gcgtgccaaa
     2041 cgttacagcc cctgtttact cgcatcttat ttatgatgtg atcccggatg gagataaaac
     2101 tgttgttcag cctgatattt taattcttga agggttaaat gtcttacaga gcgggatgga
     2161 ttatccacac gatccacatc atgtatttgt ttctgatttt gtcgattttt cgatatatgt
     2221 tgatgctccg gaagacttac ttcagacgtg gtatatcaac cgttttctga aattccgcga
     2281 aggggctttt acagacccgg attcctattt tcataactac gcgaaattaa ctaaagaaga
     2341 agctattaag actgccatga cgttgtggaa agagatcaac tggctgaact taaagcaaaa
     2401 tattctacct actcgcgagc gcgccagttt aatcctgacg aaaagtgcta atcatgcggt
     2461 agaagaggtc agactacgca aataatttgc aggggagcta atgctcccct ttcttatttt
     2521 tctgcactac gcagggatat ttcaccgccc atccagggtt ttattattcc gtcctgctca
     2581 agcaataaag cgccttgttt gtctattccg cgtgaaatgc caaatatttc tttatcacca
     2641 atgataagtt tcactgggcg attaataaaa ttatccagct tttcccagcg cgaaagataa
     2701 ggtgccaatc cttcttgttc gaagagttcc agcgccgcgc gtaattcacg tattagcatg
     2761 gccgccaacg tattacgatc gagattgatc cccgcttcct gcagcgtgat ccacccctga
     2821 ttaacgacac tctcttcaac acgacgcatt gccatgttga tcccggctcc aatgactatt
     2881 tgcgccgcat cgccggtttt gccagtcagc tccacaagaa tgcctgccag cttgcgatcc
     2941 tgcagataga ggtcattagg ccatttgaca cgaactttat ctgctcccag cttgcgtaat
     3001 acttccgcca tcacgatacc gataaccaga cttaaaccaa tcgccgccgc cgggccttgt
     3061 tccagacgcc agaacatcga caaatataag tttgcgccaa aaggcgaaaa ccatttccgc
     3121 ccccggcgac cacggccagc ctgctggtat tctgcaacgc aggcatcgcc cgatttaagc
     3181 tctccgatac gatcaagaag gtactgattc gtggagtcaa taactggcag cacggctaca
     3241 ctaccgccat ccagctgacc caatatctgt tcagcattaa gtaactggat gggctcaggc
     3301 aggctgtatc ctttacccgg aacggtaaag acatcaacgc cccagtcacg cagtgtctga
     3361 atgtgtttat taatagccgc ccggctcatt cctaacgttt cacccaactg ctcacctgag
     3421 tgaaattcgc cattcgctaa cagcgcgatt aatttcagtg gcacggtgtt atccttcatg
     3481 aaattgtctc cactgcgctc acttcacctg atgcaccaat aaagcggact tcaggctcaa
     3541 gccagacatt aaatttttca cccacttttt gtcttacatg gtgtgccagt tgcaccacat
     3601 cttcgctttt tgcattgtct tcattaatga gaactaacgc ctgttgacgg tgcaccgcag
     3661 ccccacccat ttgcatccct tttagctggc actgatcgat aagccaacct gctgccagtt
     3721 ttactgaacc atccgcctgg gggtaatttg gtgctgttgg aaattgtgac agtaatgctt
     3781 tagccgtttc agcagataca acagggtttt tgaagaaact accggcattg ccattcactt
     3841 ttggatcagg aagtttggtg gtgcgcatat gacacaccgc atcaaatact tgctgtggcg
     3901 ttactgttgt aggatccaga cgagttaagt caccatacgt tagtacaggt tgccactctt
     3961 ttggcagacg cagacctacg gctacaatgg cgaagcggtc ctggtattca tgtttaaaaa
     4021 tactgtcgcg atagccaaaa cggcactctt tggcagttaa gcgcacttgc ttgcctgtcg
     4081 ccagttcaac acaatcaaca taagcgcaaa ctcgctgtaa ttctacgcca taagcaccaa
     4141 tattctggat aggtgatgag ccgacacgac caggaattaa tgccagattt tccagaccag
     4201 gcataccttc ctgcaaagtg tattttacca ggcgatgcca gttttctccg gctcctacat
     4261 gtaaatacca cgcatcaggt tcatcatgaa tttcgatacc tttgatccgg ttgatgatca
     4321 ccgtgccgcg atagtcttcc agaaaaagta cattacttcc ttcacccaga ataagaacgg
     4381 gttgcccttc tgcggttgca tgctgccagg cattgagtag ttgttgttcg tcttcggcac
     4441 atacaatgtg ctgagcatta tgatcaatgc caaatgtgtt ccagggtttt aaggagtggt
     4501 tcatagctgc tttcctgatg caaaaacgag gctagtttac cgtatctgtg gggggatggc
     4561 ttgtagttat gacgacaggg agagtttgta gaaacgcaaa aaggcccagt cttccgaccg
     4621 ggccttctgc ttaatttgat gcctggcagt tccctactct cgcatgggga gaccccacac
     4681 taccatcggc gctacggcgt ttcacttctg agttcggcat ggggtcaggt gggaccaccg
     4741 cgcta
//
LOCUS       DAZFKF010000213         4605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFKF010000213 DAZFKF010000000
VERSION     DAZFKF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.215"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..266
                     /locus_tag="ACHKA1_005219"
     CDS             <1..266
                     /locus_tag="ACHKA1_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936711.1"
                     /note="participates in the regulation of osmotic pressure
                     changes within the cel; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFO5682738.1"
                     /translation="EERVLKNREITVRLNEPGESSMNFVVRVWSRRDDLQSVYWDVLE
                     RIKREFDSEGISFPYPQMDIHLVRSGKQAESVTHNSRSVSSRQ"
     gene            392..1612
                     /locus_tag="ACHKA1_005220"
     CDS             392..1612
                     /locus_tag="ACHKA1_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFO5682739.1"
                     /translation="MPKKIMTGIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWPDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRKEWGIL"
     gene            1843..3588
                     /locus_tag="ACHKA1_005221"
     CDS             1843..3588
                     /locus_tag="ACHKA1_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO5682740.1"
                     /translation="MTAGEKRVASRLEAFMSNECLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLLQVRRYACDTVNALPADSRL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIFPSAQTICQDDMTQS
                     VLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMAVRQHSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIESRGL
                     KSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVNSGTITDTGYDAVLV
                     DEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEGTEPEILRCASESDE
                     AVQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPYATCFDSEGKKKYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALYVAFTRATRELLVT
                     CYRENSISRHLEDFV"
     gene            complement(3770..4399)
                     /locus_tag="ACHKA1_005222"
     CDS             complement(3770..4399)
                     /locus_tag="ACHKA1_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO5682741.1"
                     /translation="MSIEQIINEINVYLNSLKKEDERIDAINKIKIELHRSSPFYAEP
                     VDCVIWIKNNLIEANDYNPNVMAPSEKRLLTHSLLVDGFTQPVVTLAQKGKYTVIDGF
                     HRQMLGKSTTKLEKKLKGYLPVTCIKHDNNFKAYSIASTIRHNRARGKHQIDALSDIV
                     RDLSRMGWDDLRIGSELGMDTDEVLRLKQISGLTELFEEENFSPAWTIK"
BASE COUNT         1173 a          866 c         1148 g         1418 t
ORIGIN      
        1 cagaagagag ggttctgaag aacagggaaa tcactgttcg cctgaatgag cctggggagt
       61 catccatgaa ttttgtagta cgggtatgga gcaggcgtga tgaccttcag tctgtatact
      121 gggatgtact ggagagaatt aagcgggagt ttgattcaga agggataagt tttccatatc
      181 cacaaatgga tatccatctt gttcgctccg ggaagcaggc agagtcggta acccataaca
      241 gtcgctcagt cagttccaga cagtaaaatg aaaagcgcat tttatttgtg aaagatgaac
      301 agcctgaatc tgtcaggctg ttttcattgt gtttttatgg tcatttttgg tatatgtact
      361 gtatataatg tcatctgtac aaggatataa aatgccaaag aaaataatga cagggattga
      421 tgtttttgac gctgccatgg agcgtattga atgggtgttt gatacgtttt catcagtttg
      481 tctttctttt tccggcggaa aagactccac tgtcctgttt cacctgatgg cgaatattgc
      541 ccgccgaaaa aaacgccgtt tttctgtact gtttgttgac tgggaggctc agtatcagtg
      601 taccattgag catgtcacca ggttgaggga aatgtacgat gatatcacag atactttttt
      661 ctgggtggcg ctgcctctga cgaccgttaa tggagtgtct caggtacagc ctgagtgggt
      721 atgttgggag gacggggctg aatgggttcg ccagccacca gaagatgcca taaccagagc
      781 gtcttttttt cctttttatc agaatgcgat gacatttgaa gagtttattc cggcatttaa
      841 tcattggttt gcccgggaaa aagggatggt tgctgcgatt cttactggtg ttcgcgctga
      901 tgagtcactg aatcgtttta tgggactggt atccggacgt aagctccgtt atgcggagga
      961 taaaccctgg accacggcat cggctgaggg attttactac accatgtatc ctttgtacga
     1021 ctggaaaaca agagatgtat ggatttacca tgcgagaaca cgtgctgcct ataactctct
     1081 ttatgacctg atgtacaggg ctggagttcc tcttcgcaat atgcgggtgt gtgagccttt
     1141 tggaccggag caacgtaagg ggttgtggct ttatcatgta ctggagccgg atacatgggg
     1201 cagaatgtgt gctcgcgtat caggtgctgt agggggagcg ctctatgcaa atgaaagtgg
     1261 tgcttatttt gctttgagga aacagataac aaaaccagtt caccatacat ggcagagtta
     1321 tgcaatgttt ctgctggagt caatgcctga gaaaacggcg gagcattaca agaataagat
     1381 tgctgtttat cttcggtggt ggcagtctca tggatggccg gatggcatcc ctgatgagca
     1441 ggataatgac cagggatgta aagatattcc ctcctggcgt cgcatctgta agacgttact
     1501 gaaaaatgat ttctggtgtc ggatgctggc gtttagtccg aataagcccc gacattatga
     1561 gcgctacctg gccagaatgc gtgaaaaaag gaaagagtgg ggaatattgt aaattatatt
     1621 taaacatcag aaatatgacg gttaatccag gatagtagct aaaaaaataa gattccttgc
     1681 tgatttatcc ctgtttgata tgtaaaatct gaggggctta taagcaacgg atatacgagt
     1741 gctacaatat atgataagtt atatatatta aatgttcatc atctgtcagg gggctgtttt
     1801 atggctaaac tgataaatat atcaggtgtt accgaggagc ggatgactgc cggggaaaag
     1861 cgggttgcat cccgtcttga ggcatttatg agtaatgagt gtctggtctg gtacgatatt
     1921 ccggtgggac ggaaaaaccg acatcctgat tttgtcatta ttgacccgga gaatggtctg
     1981 gtatttcttg aagtcaagga ctggaccgtt cacacgttgc atcaggtaaa tcacgaacag
     2041 gtcattcttg aaactaacgg tattcttaaa tcagaaataa atccgctgct acaggttcgt
     2101 cgttatgcct gtgataccgt aaatgcttta cctgcagact cacgtttgcg acagaatgat
     2161 ggtcagtata aggggcgtct gaatctggcc tgggcgtatg gtgttgtgtt tacccgtatt
     2221 acccgccagc aactgaaggc tctggctggg aatgatgaaa atgctgtgga aaggattttc
     2281 ccttcagcgc agacaatctg tcaggatgat atgactcagt ctgttttacc tgatatattc
     2341 cgccaaaaaa ttgccgggat gtttacgatg ggattcagaa cccgggtgac accacaggtt
     2401 agagatattc tgcgtgctca tctgtttcct gaaatggccg tcagacagca ctcacaaatc
     2461 aaaataatgg atattcagca ggagattctg gccagaaata ttggtgatgg gcatcgtgtt
     2521 attcatgggg tagccggctc tggaaaaacc atgattcttt tgttccgttg tctttacctg
     2581 gctgaaacta cccctggtaa gattctggtg ctatgttaca acattactct ggccagctac
     2641 ctgcgggaat gtattgagtc caggggactg aagtcacggg ttacggtatt ccattttcat
     2701 agttggtgcg cctcaatgat aaagcgccat ggaattcagg tgactgctgg aggaaaggat
     2761 tacccggaga aatgcttcag tgcgctggaa gatgccgtga acagcggaac aattactgat
     2821 actggatacg atgccgtact tgtggatgag gggcatgatt ttgagagtcg ctggctggct
     2881 ctgatagccc gcctgtttga taacgcgtcc cgttcactgc tcctgatgta cgatgacgca
     2941 cagtccattt acagacggga acgggctctg aacttttcgc ttgccagtgt ggggattcag
     3001 gcgcagggac gtacttccgt cctgccggta aattaccgta atacgaaacg cattctgcac
     3061 tttgcttatg cattctcccg tgagtatttt gagaagcatc agaaccgtga aataccgttt
     3121 gttcagccac aggcgggcgg tgaagagggc acagagccgg aaattctgcg ttgtgcttct
     3181 gagagtgatg aggcagtgca ggttgtgggc tggctggaga aacgctatac actgtgcggt
     3241 cactggagtg atatggctgt tttgtgtcct gcagagtttt cagtgaaaca tctgaaagag
     3301 gtcatgacac aacgcgggat accttatgcc acctgctttg acagtgaagg taagaagaaa
     3361 tattcgcgac gcaaggatgt tgtgcattta ctgacctacc agagcagtaa gggactggag
     3421 tttccgtatg ttgcggttat caatgcttct ttcgttcact caggtgctgc ggatgagtct
     3481 gaagttattc cggcgcttta tgttgcgttc acccgggcta cacgggaatt actggtgacc
     3541 tgttatcgtg aaaacagtat cagtcgtcat cttgaggatt ttgtataagt tgtgttttat
     3601 tattgctgat atgtaatcat tcgttttttg ataatctaag atgggtattt agcgagtatt
     3661 cagagttgaa ttaatattcc ccatgttttt gatctgtcaa tagctgtcca ttacatgggg
     3721 tgatcatata tcagtcggta attactggat aaagtcaccc cctaaatgat cactttattg
     3781 tccatgccgg actaaagttt tcctcctcaa atagttctgt taacccactt atttgtttta
     3841 atctcagtac ctcatctgta tccatgccca gttcactacc tattcggaga tcatcccatc
     3901 ccatccggga aagatctcgg acgatatcgg agagcgcgtc tatctggtgc tttcctctgg
     3961 ccctgttatg cctgatggtc gaggcgatag agtaagcttt aaagttatta tcatgcttaa
     4021 tgcatgtgac aggtaaatat ccttttagtt ttttctctag ttttgttgtg cttttcccaa
     4081 gcatttgtct gtgaaatccg tcaataacgg tgtatttccc cttttgagct aaggtgacaa
     4141 caggttgagt gaatccatca accaacaggg agtgtgttaa taaccttttt tcggagggag
     4201 ccatgacgtt aggattatag tcgtttgctt caattagatt gttttttatc catataacac
     4261 aatcaacagg ttctgcgtaa aaaggactac tcctatgtaa ctcaatcttt attttattaa
     4321 ttgcatcaat gcgttcatct tctttcttaa gagaattaag gtatacattt atttcattta
     4381 tgatttgttc aatgctcatg tgtttctatt tcttttaatt tgtatatttt tcttgtttta
     4441 agatgagttg ttcttctttc tctactttgt gttaatatat aagttgtaag gttgttttgt
     4501 gtttttattt ttggtgctgg aggagataat atgctcctca tgataaacga tgtgattttc
     4561 aaataaataa tgctgggtat aatattttct tttgaatatt aaaga
//
LOCUS       DAZFKF010000214         4553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFKF010000214 DAZFKF010000000
VERSION     DAZFKF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.216"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1202
                     /locus_tag="ACHKA1_005223"
     CDS             <1..1202
                     /locus_tag="ACHKA1_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5682742.1"
                     /translation="SYQVAYNKYLLQTATHFSVAAWRYASQDYRTFSDHLYENDKINH
                     QSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNAKDYQFSYSNNW
                     QHISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLSNSTSFSKDGYS
                     SNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATIDGSYSHSKNAWQ
                     SGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLM
                     IPHRENHLVLDTANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGS
                     PLTFGYDVLDLQENNIGVVGQGSLLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDE
                     NKTYICQ"
     gene            1215..2174
                     /locus_tag="ACHKA1_005224"
     CDS             1215..2174
                     /locus_tag="ACHKA1_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO5682743.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCGLTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLLETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNFNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(2212..3111)
                     /gene="nhaR"
                     /locus_tag="ACHKA1_005225"
     CDS             complement(2212..3111)
                     /gene="nhaR"
                     /locus_tag="ACHKA1_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO5682744.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(3177..4343)
                     /gene="nhaA"
                     /locus_tag="ACHKA1_005226"
     CDS             complement(3177..4343)
                     /gene="nhaA"
                     /locus_tag="ACHKA1_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO5682745.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1365 a         1059 c          955 g         1174 t
ORIGIN      
        1 cgagctatca ggtcgcctat aataaatatc tattgcaaac ggccactcac tttagcgtgg
       61 ccgcctggcg ttatgcttcg caggattaca ggacattcag cgaccatctt tacgaaaatg
      121 ataaaataaa tcatcagagt gactatgatg atttttatga tattggcaga aaaaatagcc
      181 tttctgccaa tataatgcaa cctttatcca ataatctggg aaatgtatca ttaagtgcac
      241 tttggcggaa ttactggggg cgaagtggaa atgctaaaga ttaccaattc agttactcca
      301 ataactggca acacattagt tatactttct ctgccagcca atcctatgat gaaaacgata
      361 aagaagagga gcgttttaat ctgtttatct ccattccttt ctactggggt gatgatattg
      421 ccaaaacacg tcaccaaatt aacttatcga attctacctc attttccaaa gatggctatt
      481 cctccaacaa tactggaatt actggcatag ccggtgaaca tgatcagtta aattatggta
      541 tatatgttaa tcagcaacaa caaaataatg atacctcgct tggtacgaat ttaagctgga
      601 gaactccaat cgccacaata gatggcagct atagccattc taaaaacgcc tggcaaagtg
      661 gtggaagtat tagtagtgga ttagttgtct ggcccggtgg tattaatatc actaaccagc
      721 tatccgatac atttgcaatt ctggatgcgc ctgggttaga aggcgcgcat attaatggac
      781 aaaaatacaa ccgaacaaac agcaaaggcc aggttgttta cgacctgatg atacctcatc
      841 gtgaaaacca tctggtactt gatacagcaa acagtgaaag tgaaacagaa ttgcagggca
      901 atcgtcaaat tattgcgcct taccgtggag cagtttctta tgtgcagttt acaactgacc
      961 aacgtaagcc ctggtatata caggcactgc gtcccgatgg ttcgccatta acttttggct
     1021 atgacgtact ggatctccag gaaaacaata ttggagtcgt tggccagggt agtctccttt
     1081 ttattcgcgt agatgaaatt cccactggca taaaagttgc tctcaatgat gaacagaatt
     1141 tattctgtac tattactttt caacacgtta tcgatgaaaa caaaacatat atatgccagt
     1201 aaaaggagtt tactatgaag tggcttttat taatcacctt gagtctatat tcttttattg
     1261 tgcaaagtgc tccctgtgga ttgaccaatg tcggggaaca acgtggaaca tatatactca
     1321 agccactgag tatgaaaggt aatctcaccg ccgaaaaaat tgaaaacacc ggaaatatca
     1381 ttgagtgtgc agacttgcta gagacgacta acgccattaa agttcatttt ttaacacaga
     1441 acgctttaag gtttaaagtc ggaagtcgtc attatattat taatttcccc tttgaatctg
     1501 acggtaaaaa aatagagctt ccagggaagt catcttataa tttagaagag atgctatctg
     1561 cgataaattt caccattcca tactctatcg catctgtatc agatagttcc aacaccgcag
     1621 atatttttcc aggaactcct tttcctttgt tgttcgaaat tgaagtgagt tcatgtacag
     1681 ggaataatac taattgtaat agcgtccgct ttaattttaa tttaaatact attttacagg
     1741 taaatttaat gacctgtggt tttgaagatc aatccatcga cactggacga tttgtactgt
     1801 ctcgtatcaa taatgaaaat tatcagtttc atcatataag atttgactgc atccagggcg
     1861 agcaaggtga attagttttt gaacccagtg acgttgaata ttactttgaa ccagtgacga
     1921 tacaggagtc aggaaccagc atattaaaaa atgagttaga aaacagtgag gacggtgcgg
     1981 gtcaaatagg ctttcaactc tccaatgatg gtacgaatga aatccagtat ggtaaaagca
     2041 attactactc ttttcagcat ccccatgaag gaagtaatca gattcctcta tttattcgcc
     2101 cgcgtacata tggaaacaat gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg
     2161 taatgtacaa ctaatctatc tcccccacct taactttggg ggagctgccg attaacgcac
     2221 cgctggacta aaaagcgcag aataatccgt attgcagatt cgctgtaccg ccgggtgctg
     2281 aatcatccgc tcagcaaaaa tagcatggta ctcttccatc acattctcga cgcgaccaat
     2341 ttctacgaca gttttatcgg cataaaagtc atatgcataa agcgttgggg caacgaagat
     2401 tgcattgtgc atcgcaccaa aagctttcat caaagcggca tcatcaaact cgccaaggat
     2461 ttctacgttt aatccctggg agttaaacca gttaagtaat ttgcgcccta acattgaacg
     2521 tcgcccagga atcaaaagcc gccgttcttc aagacaagct gggaacggtt tttccggcgg
     2581 tggatttgta caccagaaac tcacgccaca ttcgccaatt ctcacggaga acaggccttc
     2641 ctgctgcgta gaatctatcg gacagtcgga aatgatcata tccagtttat gctggcttaa
     2701 ttgctccagc agcatttcgt gggtggattc gaagcagcga agatgaatcg gctcgccttc
     2761 tactactgca gcattcagca cgctactgac cagacgtttg gaaagtgcat cagccacgcc
     2821 aacgtcaaac aataaattgg attctttgcg atagttcacg atatccagca tttcctggct
     2881 caaggtgaac attttatcgg catagcgata gaccagctcc cccagctcgc tgggctcgag
     2941 accacgtccc ttgcgcttaa ataatttgcc ttgcaggcgc tcttccagcg cccgaatctg
     3001 tccggtaatg gtttgtggtg ttaaataaag cgcctccgct gcgccaacca cagaaccttc
     3061 tttataaaca tgccagaagt aatacaagtg gttgtaattg atatgagaca tgctcatttc
     3121 tctccctgat aacaatgaaa agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa
     3181 actgatggac gcaaacgaac gcgtaaccag ctgtatccaa ttaccgccga agagatagaa
     3241 ccgaccagga taccgagttt cgcccagtta atcagttctg gatctacgct accaaaggcc
     3301 aggctggcaa taaagataga catagtaaaa ccgataccgc acaggatccc caccgccata
     3361 atttgctgat aagtcgttcc ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag
     3421 cagaacagac taatccccag cggtttgcca atcagcaagc cagcgatgat ccccaatggc
     3481 agaatggagg tcaagccatc cagcgtgacg ccttgcagtg aaacgccagc attagcaaat
     3541 gcaaacagtg gtaaaatcag atacgccacc cacgggtgca acacatgctc cagtcgcttc
     3601 gctggagaac gcccatgctt ctctttcaaa ggaataaaga agccgacaat tacccccgcc
     3661 agggttgcgt gaacccccga tttcaacact gcagtccaca acactacgcc aaccagaata
     3721 tagacgcccg tgcggcgtac accacacaga ttcaataccg cgagtaccgc aattgctaca
     3781 gccgcgacgc caagagaggc catcgataag tcattagtgt agaacaatgc gatgataatg
     3841 atggccccaa gatcgtcgat aatagccaga gccatcaaaa agatcttcag cgctaacgga
     3901 acacgacttc ccaatagcgc cagcacacca agtgcaaagg caatgtcagt agccgccggg
     3961 atcgcccacc cttcgcgggt aatcggatcg gcatagttaa aggccagata gagtaatgcc
     4021 ggcacaatca tcccaccaat agcggcgata actggaaatg cggcctggcg taagctggct
     4081 agcgatcctt gcatcagttc acgtttaact tccagaccga ctaacaggaa aaataccgcc
     4141 atcagcgcgt catttatcca taacagcatg tttttgttga tttcgagtga accaacccgg
     4201 agctgaaccg gcgtctccag aaagtcgtga taccatccac tggttgcgcc gctgttggcc
     4261 atcatcatcg ccaggatagc ggcaatgata agaataatgc ctcccgaggc atcactgcta
     4321 aagaatcgat gcagatgttt cactttttat ttctctttca ggtgaataga tcgtttttac
     4381 tattttaccc cgcacgaatc atcgttaaaa tcagattata gtgtaggaat aattcgtttt
     4441 ttacgatcta tagaagaatt tgcgccatca gcggtgagat gcgtttttga cggctgtaag
     4501 cgctgacggg tgaccctaag ccctgataat catagcttaa ggtgaggatt tct
//
LOCUS       DAZFKF010000215         4509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFKF010000215 DAZFKF010000000
VERSION     DAZFKF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.217"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(355..636)
                     /locus_tag="ACHKA1_005227"
     CDS             complement(355..636)
                     /locus_tag="ACHKA1_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682746.1"
                     /translation="MKTNKKGTKWHIFYRENSGAEVLLEIPSFRECLSVSKELMTPSN
                     YMICIEKNGERIKRWDREIIAGSNKWINCPPDNFEILGELITINRIIKK"
     gene            complement(694..1086)
                     /locus_tag="ACHKA1_005228"
     CDS             complement(694..1086)
                     /locus_tag="ACHKA1_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682747.1"
                     /translation="MELTVDLLKSLNNIRQNATIYVHAYHYTDDEKCAMVPRISLPSL
                     KLTPLQSEIIIRLLLNGVDYIRHDGYVQGYREKTVATFTIQGEGRHRFKADLSQNGES
                     DIASNVLNHWLHRIHKTDIIVLSVVLTE"
     gene            1703..1930
                     /locus_tag="ACHKA1_005229"
     CDS             1703..1930
                     /locus_tag="ACHKA1_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HFO5682748.1"
                     /translation="MFPEYRELISALKGNHKRFDSLFEKHNKLDHEIKRIEDEFGSAY
                     STKVVRLKLEKLEIKREIQKILEDESEKGER"
     gene            2308..2883
                     /gene="dolP"
                     /locus_tag="ACHKA1_005230"
     CDS             2308..2883
                     /gene="dolP"
                     /locus_tag="ACHKA1_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO5682749.1"
                     /translation="MRLLYSAVVLISALLLQGCVAATVVGTAAVGTKAATDPRSVGTQ
                     VDDEVLELRLSSALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADVASRVSGVKRVTTAFSYIR"
     gene            complement(3313..4497)
                     /locus_tag="ACHKA1_005231"
     CDS             complement(3313..4497)
                     /locus_tag="ACHKA1_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO5682750.1"
                     /translation="MRKISVSYICKSLFLYCSASLPVLSANGISDKMIVELGDNSAAK
                     ANFAVAVGRYSQANAEKSISLGASARAIENNSTALGANATTLKPFSVALGAGSVADRG
                     AGIEAYMPETKRWNPGDVVKSRMSDKQLNRLMEIDKIISAYNSQGTLSAEETKQLEEV
                     KKERADMFATYMATHDAVSVGVGATTTGRHAVTRQITSVAAGSVDTDAVNVAQLRDLQ
                     TVTEKADAALDKRITNNTSAITKNLQVVNSNISENSKQISTNTLGIRENTQRIRDEGQ
                     ARIRGDRETLRAATGYTDMRVNGLERNTNKRFAQLKEDIDKNRKRADAGIAGAMAITG
                     IPSVPGKKVSFGMAMAGYRSEGAVAAGFHFNTSENSAVKVNAAWDTENGAGVSAGMAL
                     GW"
BASE COUNT         1196 a          977 c          990 g         1346 t
ORIGIN      
        1 ttatcctcct tatctgcgct gatacctgta tataaaaaca gtgcaacatc aatttaagaa
       61 ttaaagaaga taataaaagt caattaaatc agcatgatgt ctgtgttttg aatgtgtggg
      121 gtttttgggg cgagtaatgt acgcgcgtat tctctagagg tttaatttaa aaggctacgt
      181 atgtagcttt atgtatttaa gagtactgaa ctcagtctcc ggcaataaac cagtttggcg
      241 tccagctaac aaactcttgc ggatgaaagg tccgggcaca gcgatgactt acaagcattc
      301 tcccgcaaaa aatgaactat agcgccttgc ttaatgcatt gtctgaatcc ggagttactt
      361 tttaataatt ctgttaatgg ttattagctc acctaatatt tcgaagttat caggtgggca
      421 gttaatccat ttgttagagc cagcgatgat ttccctgtcc cagcgtttta ttctttcgcc
      481 atttttttct atgcatatca tgtaatttga tggggtcatt aattctttag atactgaaag
      541 acattcccga aatgatggta tttcaagcaa aacttccgcg cctgagtttt cacggtaaaa
      601 aatgtgccat tttgtgcctt ttttattagt tttcacgtgc gtatgctcat aggataaata
      661 taaaaccttg taacattcag gaggtaacag gatttattct gtaagaacaa cagacagaac
      721 aatgatatct gttttatgta tacgatgtag ccagtgattt agtacattgc tggctatatc
      781 actttcccca ttttgtgata agtctgcttt gaaacgatgg cgtccctccc cctgtattgt
      841 gaaagttgcc acagtttttt ctctataccc ctgaacatat ccgtcgtgcc ttatataatc
      901 gactccattg agaagcagtc gaataattat ttctgattgc aacggagtta atttcagtga
      961 tggtaaagat atccgtggca ccatagcgca tttttcatca tcagtatagt ggtatgcgtg
     1021 aacatatatg gtggcatttt gcctgatgtt gttaaggctt ttcagcaggt ctacagtcaa
     1081 ctccatcgcg gtatccttca ggttgttatc attcgttgat gatgcgatct gttgaaggcg
     1141 ttggcgacaa ttaattcttt ttctcttctg aaaaatttac ggaatacaaa aaagatctgt
     1201 ttttaactga ttgttacttt ctgaagatat tctctgctcc atttgctttc cgggtaagac
     1261 gtatggctgg ttatggtatg gtcggactgc agtcacaggg ggatgactgt aatacctctg
     1321 atcaccacag tcagtcatct gtaaatgtta aatacctcac agttcagtca gtggattatt
     1381 gtcggtctta cgggatattt gttaaaaaaa tactgcatgg tgaatccccc tgagcggcgg
     1441 ggcatatcag tatcgtaggt gtttggtcac atcccttatc tgtgcgggtt caggtgctga
     1501 tactgaactc accgggaggc acccggcacc atgctacata acactccatt acgctacact
     1561 cgcccctctc cggaggggtt ttctctgcag gatgtgaata tgagatgctg catcattcac
     1621 tgcgaatatt gatggtgaga atctttaata atattaatgt ttatatgtac tgttacagtg
     1681 ttgatgaaag gagataaaaa caatgttccc ggagtacaga gaactgattt cagcattgaa
     1741 aggaaatcat aaacgctttg attctttatt tgaaaagcat aataaacttg atcacgaaat
     1801 aaaaagaatt gaagatgaat ttggttcagc gtattcgacg aaagtggtca ggctgaagct
     1861 tgaaaaactt gagatcaaaa gagaaatcca gaaaatcctt gaagatgagt cagaaaaggg
     1921 cgaacggtaa tcataaacgg tatattcaaa gacgtgtttt ctctgtttta tgaaaaaggc
     1981 accggggact ccacggtgcc ttttagcctg tgtataacga aaaggagatc ctgttcagcg
     2041 aaccagatag ttaaaaaaag aatctttacg acggtatcaa agttttcgtg caaaaaacat
     2101 gatcaatatt aaattccagt acatccatat ccgatataaa tctttccgtc tgacagatac
     2161 agatatgcca cgtaacgcat ccgctgtttc cggctgtgcc ggaggataat aacagtctgt
     2221 agcttgtttt gtttcactga aggtaaactt tttgtacgat aaattccggg aatatgaagt
     2281 aaaaatcaaa acaattccgg gggagatatg cgtttattat attctgctgt tgtcctgatt
     2341 tcagccttat tgttacaggg atgcgttgct gcaactgtgg tgggtacagc cgctgtcggg
     2401 acaaaggctg caaccgaccc acgcagtgtc ggaacgcagg tggatgatga agtgctggag
     2461 ctgaggctca gcagtgcact ttcaaaagat gaacaaatca gaaaagaagc acgcatcagt
     2521 gagattgcat atcagggacg cgtgctgctg atcggtcagt ctccggaccg tgaattatct
     2581 gcccgtgccc gtcagatagc actcggtgtc gatggtgttc aggaggtcta taatgaaatc
     2641 cggcagggga cacccgtcag tccggtcata atggcaggtg atgcctggct tacgacaaaa
     2701 attcgttctc agttactcac aagtgcccgt gtcaaatcat ccagtgtcaa ggtggtgaca
     2761 gaaaacagcg aagtcttttt actgggcctg gttacagagc aggaagggcg agcggcagca
     2821 gatgttgcca gtagggtgag cggagtgaag cgggtcacga cggccttctc ctatatccgg
     2881 tgaaggcagt atcagagttg cctttagtgt aaatttacgt taaaaatcag aagagcacgt
     2941 ttccgggccg ttcatgccac cacgtgaaca ttcgtcgcgg aataccggag gcgtgctcat
     3001 tataataacc aatatacata attcctcttt gacacccccc tgaattcggg ggattttttt
     3061 gcccgggtat gttcactcac agataaaatc ccggtgcgct ccggatgtcg ctgctggtaa
     3121 ataatctgag cttttatcca tacaggtcaa agagcgtggc gttgactcgt gtacgtctgg
     3181 ctccggtgca ggagccagat tgtccgtatt aattacatcc tgagccgatg cgataaactg
     3241 aatcgcttcc tgaatggtgg tgcagtgtcc cctggataac aatatctgcg aacggcttct
     3301 ggcccggaag cattaccatc ccagagccat cccggctgaa acgccagctc cattttcagt
     3361 gtcccaggct gcattcactt taacagcact gttttcactt gtattaaaat ggaagccggc
     3421 agcaacagca ccttcactcc ggtatccggc catggccata ccaaatgaga cctttttccc
     3481 ggggacggac ggtatccccg ttattgccat cgcacctgca atacctgcat ctgcacgttt
     3541 tctgtttttg tctatatcct ctttaagctg cgcaaaacgt ttattcgtat tacgctccag
     3601 cccgtttacc cgcatgtcgg tataaccggt cgctgcacgc agtgtttccc tgtctccccg
     3661 gattcgagcc tgtccttcat cccggatccg ttgtgtattc tctctgatac caagagtgtt
     3721 ggtactgatt tgtttgctgt tctccgaaat attactgtta accacctgca gatttttagt
     3781 tatcgcactg gtgttgtttg ttatgcgttt atcaagcgct gcatctgcct tttctgtcac
     3841 agtctgcaaa tccctgagct gtgcaacgtt aacagcatcc gtatcgacag aacctgctgc
     3901 aacgctagtt atctgtcttg taacagcatg tcgtcctgtt gtcgttgcac caacacctac
     3961 agatacagca tcgtgcgttg ccatatatgt cgcaaacata tcagcccgct cttttttcac
     4021 ttcctccagt tgtttagtct cttcagcaga cagcgtccct tgtgaattat aagcggatat
     4081 tatcttatcg atttccataa gcctgttaag ctgcttgtca gacatcctgg attttactac
     4141 atcccctgga ttccagcgct ttgtctctgg catatatgct tcaataccag cgccacgatc
     4201 agcaacactt cctgcgccca gagcgacaga gaatggtttc aatgttgttg cgtttgctcc
     4261 taacgcggta gagttatttt caatagctct tgctgatgca cctaaagaaa tacttttttc
     4321 tgcatttgcc tgtgaatatc gaccaacagc aacagcgaaa ttagcttttg ccgcactgtt
     4381 atcaccaagt tccacgatca ttttatcaga aataccattt gcagataaga caggtaaaga
     4441 agctgagcag tagagaaata atgatttgca tatataactg acactgattt tacgcatttg
     4501 aattcacca
//
LOCUS       DAZFKF010000216         4427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFKF010000216 DAZFKF010000000
VERSION     DAZFKF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.218"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            102..665
                     /gene="fimA"
                     /locus_tag="ACHKA1_005232"
     CDS             102..665
                     /gene="fimA"
                     /locus_tag="ACHKA1_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5682751.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            1026..1100
                     /locus_tag="ACHKA1_005233"
                     /pseudo
     CDS             1026..1100
                     /locus_tag="ACHKA1_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1091..3334
                     /locus_tag="ACHKA1_005234"
                     /pseudo
     CDS             1091..3334
                     /locus_tag="ACHKA1_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            3348..3878
                     /gene="ydeS"
                     /locus_tag="ACHKA1_005235"
     CDS             3348..3878
                     /gene="ydeS"
                     /locus_tag="ACHKA1_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5682752.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            3891..4394
                     /gene="ydeR"
                     /locus_tag="ACHKA1_005236"
     CDS             3891..4394
                     /gene="ydeR"
                     /locus_tag="ACHKA1_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5682753.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPAETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
BASE COUNT         1261 a          939 c          997 g         1230 t
ORIGIN      
        1 aacaaccaat caagaatgat tcttgtttta taagcaataa cagaatgact taattgttaa
       61 ctctctttat tcagctactt aaatataaat tttggagaat tatgaaactc aaacatgttg
      121 gtatgattgt cgtttctgtg ttggcgatgt cgtctgctgc ggtaagcgca gccgagggtg
      181 atgaatcagt aacgaccact gttaatggcg gtgttattca ttttaaaggt gaagtggtaa
      241 atgccgcttg tgcgattgat tccgaatcaa tgaaccaaac ggttgagctg ggtcaggttc
      301 gttcttctcg cctggctaaa gcgggtgacc tcagctccgc cgttggcttc aatatcaagc
      361 tgaatgattg tgataccaat gtttccagta atgcagctgt tgcattcctg ggtactactg
      421 tcaccagtaa tgacgatacg ttagcgctgc agagttcagc ggcaggctct gcccaaaatg
      481 tcggtatcca aattttggac cgtacgggtg aggtattaat acttgatggt gccactttta
      541 gcgctaaaac tgacttgatt gatggcacga atatactgcc gttccaggct cgttatattg
      601 cactcgggca gtccgtagct ggtactgcaa acgcagatgc gaccttcaaa gttcaatatc
      661 tataattcaa tcttaaggtg gctatacgtc atctgaaatt gaaacagggt gtgtgtggca
      721 tggctacgca cacccgagca attcaccaat gtgaatctca ttattaatga ggagtggttt
      781 gtatttggag cgctatgaaa aatcacataa tatctaaact atcaataaac aacagtagtt
      841 aaacttactg ttatttaaaa tatttacttt gtagcaaggt tttcctatta aaagagaacc
      901 aacatttatt acaaacgcat taagtctgga aacccggcga ctcgccaatg tattagcaac
      961 atttaccgat tgtctgatgt aacggttttc ccgcttatcc tcttaacaca tggagaatgc
     1021 atcagatgca aactacgcgt accccctact ctatttcctt tatggcgaca gtgttacttt
     1081 tattgctctt tgcacaccac ttcaggatcg tttggctgac gcatcaactg agtttgatgt
     1141 tggtcagcaa catttgtcac tctccgttcc gcaaatttat gttggcagaa tggctcgcgg
     1201 ctatgtttct cctgatctgt gggaagaagg aataaatgca gggcttctaa actatagttt
     1261 taatggtaat tctattaata atcgtagtaa ccataatgca ggaaaatcca actatgcata
     1321 tttgaattta cagagtggca tcaacattgg tagttggcga ctgcgcgata actcaacgtg
     1381 gagttataac agtgggagta gcaattcatc tgacagcaat aaatggcagc atatcaatac
     1441 gtcggctgaa cgtgacatta ttcccttacg ctcacggtta acggtaggtg atagttatac
     1501 caatggcgat atttttgata gtgtgaactt tcgtggcctc aaaataaatt caacagaagc
     1561 gatgttgccc gatagccaac atggtttcgc tccggtgatt catggtattg cccgtggcac
     1621 cgcacaagtg agtgtaaaac agaacggata cgatatttat cagactactg ttccacccgg
     1681 cccttttact attgatgata tcaactctgc ggccaatggt ggtgatctgc aagtaaccat
     1741 aaaagaggca gacggcagta ttcagacatt atatgttcct tattcgtctg ttccggttct
     1801 ccaacgtgct ggatatacgc gttatgcgct tgccatgggg gaatatcgta gtggaaataa
     1861 cctgcaaagc tcccccaggt tcatacaagg tagcttgatg catggactgg aaggaaactg
     1921 gacaccttat ggcggaatgc aaattgcaga agattatcag gccttcaacc ttggtattgg
     1981 taaagattta agactttttg gtgccttttc tttcgatatc acgcaggcca atacgacact
     2041 tgcagatggc acccgtcaca gcgggcaatc ggttaaatcc gtctacagca aatccttcta
     2101 ccagacagga accaatatcc aggtcgcagg atatcgctat tctacgcaag gtttttataa
     2161 cttatccgac agtgcctaca gtcgaatgag tggttacacc gtcaagcctc ctaccggaga
     2221 cagcaatgag cagacacaat ttattgatta ttttaatctg ttctacagta agcgtggtca
     2281 ggaacaaata agcatctctc agcagcttgg aaattacggt acgacatttt tcagtgccag
     2341 tcgccaaagt tactggaaca cgtcacgcag cgaccagcaa atatcatttg gattaaatgt
     2401 gccgtttggt gatattacga cttcactgaa ttacagctat tccaataata tatggcaaaa
     2461 cgatcgggat catttactcg cttttacgct taatgttccc ttcagtcatt ggatgcgtac
     2521 agacagtcag tcggcatttc gtaattcaaa cgccagttac agtatgtcaa acgatttgaa
     2581 aggcggcatg accaatctat cgggggttta tggcactctg ctgccggata ataacctgaa
     2641 ttatagcgtt caggtcggta acacccacgg aggtaataca tcgtctggca ccagtggtta
     2701 cagttctctt aattatcgtg gagcttacgg taatacgaat gtcggttaca gtcggagtgg
     2761 tgacagcagc cagatctatt acggaatgag tggtgggatt attgctcatg ctgatggcat
     2821 cacctttgga cagccgctgg gcgacacaat ggttctggtt aaggctcctg gcgctgataa
     2881 tgtcaaaata gagaaccaga ccggaattca taccgactgg cgtggctatg caatattacc
     2941 atttgcgaca gaatatagag aaaatcgtgt cgctcttaac gcgaattccc ttgcagataa
     3001 tgttgaactg gatgaaaccg tagtcactgt catcccaact cacggtgcta ttgccagagc
     3061 aacatttaat gcacaaatcg gcgggaaagt attaatgacg ttgaagtacg gtaataaaag
     3121 cgttccattc ggtgcaattg tcactcacgg agagaataaa aatggcagca ttgtcgcgga
     3181 aaatggtcag gtttatctga ctggacttcc acagtcaggg aaattacagg tttcatgggg
     3241 caatgataaa aactcaaact gtattgtcga ttacaagctt cctgaagtct ctcctggaac
     3301 cttgctgaac cagcagacag caatctgtcg ctaaggaaca atagattatg aaatacaata
     3361 acgttatttt cctcggttta tgtctggggt taaccaccta ttctgcttta tccgcagata
     3421 gcgttattaa aattagcggg cgcgtcctcg attatggctg cacagtctca tcggattcgc
     3481 ttaattttac cgtagatctc caaaaaaaca gtgccagaca atttccaacg accggtagca
     3541 caagtccagc cgtccctttt cagattacgt taagtgaatg cagcaaaggg acaacggggg
     3601 ttcgggttgc atttaacggt attgaggatg cagaaaataa tactctgttg aaactggatg
     3661 agggaagcaa tacggcctcc ggtttaggta tagaaatact ggacggaaat atgcgtccgg
     3721 tgaaactgaa tgaccttcat gccgggatgc agtggatccc actggtacca gaacagaaca
     3781 atattttgcc ttactccgct cgtctgaagt caactcagaa gtccgtcaat ccgggactgg
     3841 tgagggcttc ggcaaccttt acccttgaat ttcaataatc agagtcagat atgaaaagac
     3901 ttcacaagag gttcctgtta gctacgtttt gcgcgttatt cacagcaact ctccaggccg
     3961 ccgatgtcac tatcactgtt aatggtcggg tagtcgctaa accctgcact attcaaacca
     4021 aagaagctaa cgttaatctc ggggatcttt atacgcgcaa tctgcaacaa cctggttctg
     4081 catctggctg gcacaatatt actttgtcat taaccgattg tccggctgaa acaagtgcag
     4141 tgacggcaat cgtgacaggt tcaactgaca atacgggtta ttacaaaaat gaaggtactg
     4201 ccgaaaatat tcagatagag cttagggatg accaggatgc gacgttaaaa aatggcgata
     4261 gcaaaacggt tattgttgat gagatcactc gtaatgcaca gtttccactt aaggcaagag
     4321 ctatcacggt gaatggaaac gcaagccagg gaacgatcga ggcgctaatc aatgtgatct
     4381 acacctggca ataacgtcga ttcgattttc ttagccttct ttttaaa
//
LOCUS       DAZFKF010000217         4405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFKF010000217 DAZFKF010000000
VERSION     DAZFKF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.219"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..982
                     /locus_tag="ACHKA1_005237"
     CDS             <1..982
                     /locus_tag="ACHKA1_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO5682754.1"
                     /translation="DYEFNLAPDKYCQHIAKTNTLIFSKLKSNEVINKSLEQMSQTLF
                     KSWFVDFDPVIYNALDAGNPIPEALQSRAELRQKVRNSTDFKPLPAEIRSLFPSEFEE
                     TELGWVPKGWSIVRTEDIALKIGMGPFGSNIKVSTFVNAGVPIISGQHLKALLLIDGD
                     NNFITPEHAEKLKNSAVYRKDIIFTHAGNIGQVSLIPEDSEYDRYIISQRQFFLRVNE
                     SKSSPYYLIHYFRSEKGQHALLSNASQVGVPSIAQPSTHLKNISFLNPPMVLLKEFEK
                     FSTPLFHRFSKNRKCGVSLTALRNTLLPKLISGELSLEDLPDLSTDTEAA"
     gene            1077..4331
                     /locus_tag="ACHKA1_005238"
     CDS             1077..4331
                     /locus_tag="ACHKA1_005238"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFO5682755.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVLLQQAAELPPSYINPPLNLIKIVNQLKISGKD
                     NQSRIPDGILYINGLPLVVFEFKSAVREQDASIGNAWRQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKARHEVKICCRYPQYYAARKLYYSIKQARKPFGNGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTILPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERSNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVKGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVFLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVTEVKNHLDLLGQVFYDFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDHIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFRKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKKNHMS"
BASE COUNT         1330 a          971 c          958 g         1146 t
ORIGIN      
        1 agattatgaa tttaatcttg caccagataa atattgccaa catatcgcaa aaacaaacac
       61 tcttatattt agtaagttaa aatccaatga agtaataaat aagtcattag aacaaatgtc
      121 ccaaactctc ttcaaatcct ggtttgtgga ttttgatccg gtgatttata acgctctgga
      181 tgcaggaaat ccaatcccgg aagctctgca atctcgtgcc gaattacgtc aaaaagtacg
      241 taatagtaca gattttaaac cgcttccggc ggaaatccgt tcgcttttcc caagtgaatt
      301 tgaagaaacg gagttgggtt gggtgccgaa aggatggagt attgttcgaa ctgaagatat
      361 tgcattgaaa ataggaatgg gaccatttgg ttccaatatt aaagtatcca catttgttaa
      421 tgctggtgta ccaattataa gcggccaaca tctgaaagcc ctccttctta tcgatgggga
      481 taataatttc attactccag agcatgctga aaagctcaaa aactctgctg tatatagaaa
      541 agacataatt tttacacatg caggtaatat tggccaagtt tctttaattc ctgaagattc
      601 tgaatatgac agatatataa tttcccaacg tcaatttttc ttacgcgtaa atgaatcaaa
      661 atcatcgccg tactatttga ttcattattt taggtcagaa aaaggacaac atgctctgct
      721 ttctaacgcc tctcaggttg gtgttccttc aattgctcag ccttcaacac atttgaaaaa
      781 tatatcattc ctaaatcccc caatggtttt gcttaaagag tttgaaaaat ttagcacccc
      841 tttattccat cgctttagta aaaatagaaa atgtggagtc tcactaacag ccctccgcaa
      901 caccctgctc ccgaaactta tctccggtga gctatccctg gaagatcttc cggatctcag
      961 caccgataca gaagccgcat aacgcatttt gcccctgtaa aatcaggggc tttctggtaa
     1021 ggttttctac tgatacagga atgcttacca gaaattagcc agggttggag cgcgatatga
     1081 gtctctcttt cagtgaagca aaattagaac aagcgatcat tgaactgtta caggatcagg
     1141 ggtatcaaca tctgatcggc gataatgtcc cacgttcgag tctcgatcag gtcattatcg
     1201 aagacgatct ccgtcattat ttagcggcac gctaccagcc tgatggcatt actgaagaag
     1261 agattcagcg actgatcaaa cagttcacca cgcttccggc ttccgatctt tatgaaagca
     1321 acaaaacatt ttgtcgctgg ctggcaaatg gttttctgtt caaacgcgac gatcggcaac
     1381 aaaaagatct ctacattgaa ttgctcgaca cccggcatct acctgccgca ctgcgccaga
     1441 tatttgacgc cgaagatgtc ctgttgcaac aggctgcgga actcccgccc tcctatatta
     1501 atccgccgct taacctgatt aagattgtta atcagcttaa aatctccggc aaagataatc
     1561 agagtcgtat tcctgacggc attctctata tcaacggtct gccactggtc gtctttgaat
     1621 ttaaaagtgc ggtgcgcgag caggatgcta gtattggcaa tgcctggaga caactctgca
     1681 aacgctatcg ccgggatatt ccgcaactgt ttatctacaa cgcgctctgc attattagcg
     1741 atggagttaa taaccggatg ggcaacctgt ttgcgcccta tgaatatttt tactcatggc
     1801 gaaaagtcac cggtaatgaa aaccgtgaac aggatggaat tccatcattg cactcaatga
     1861 ttcaggggct gtttcatccg gtacgtctgc tggatgtaat taaaaacttt atctgcttcc
     1921 cggataaagc caggcacgaa gtaaaaattt gctgccgata tccgcagtac tatgccgccc
     1981 gcaaactcta ttacagcatc aagcaagcgc gtaaaccttt cggtaacggt aaaggcggca
     2041 cttactttgg cgcaacgggc tgtggcaaaa gttacaccat gcaattttta acgcgtcttt
     2101 tgatgaagag cgtagagttt gccagcccga ccattgtttt gatcaccgac cgcaccgatc
     2161 tggacgatca gctttctgcg caaatgtgca acgccaaaaa ttacattggt gacgacacca
     2221 tccttcccgt taccagccgt gaagatttgc gtaatcaact ggcgggacgc aatagtggcg
     2281 gtgtattcct gacaacgatc cataaattca ccgaagacac cgaactcctt tcggaacgca
     2341 gcaatatcat ttgcatctcg gacgaagcac atcgcagcca ggttaacctc gaccagaaag
     2401 tcatcgtcga taaagaaagc ggaaaagtgc gcaaaacgta tggctttgcg aaatacctgc
     2461 acgattcact gccaaacgcc acctatgttg gctttaccgg cacaccgatt gacgcgacgc
     2521 tcgatgtctt cggtgaggtg atcgacagtt acaccatgac cgaatccgtt caggatgaaa
     2581 tcactgtacg catcgtgtac gaaggccgtg cggctaaagt gatcctggac tccagcaaac
     2641 tggaagaagt cgaaaagtat tacgaagagt gcgcaaacgc aggcaccaat gagtggcaaa
     2701 tcgacgaaag caaaaaagcc accgcaacca tgaatgcggt tctgggtgat gaagatcgat
     2761 taaaagccct cgcggaagat tttgccaaac attatgaaaa acgcgtagcc gaaggttcca
     2821 ccgtaaaagg caaagccatg tttgtttgtg ccagccgtga aattgcctgg gatttctacc
     2881 gccagcttaa agctattcgc cctgcctggt ttgaagtgaa gcaagccccc gatggcgtct
     2941 tcctgacaga acaggagcaa aaagagttac cgccttctga aatggtgaag atggtcatga
     3001 cgcgcggtaa agatgacgac gaggcgcttt atgatttact gggcacaaaa gaatatcgca
     3061 aagagctgga taagcagttc aaaaacgcta aatcgaattt caaaattgcc attgttgttg
     3121 atatgtggct gaccggtttt gatgttcctg aactggatac catctatatt gataagccct
     3181 tacaaaaaca taaccttatc cagactattt ctcgcgttaa ccgtaaactg gaaggcaaaa
     3241 gcaaagggtt agtggtggac tacatcggca ttaaaagtca gatgaaccag gcactggcaa
     3301 tgtattcccg cattgatgcc accaactttg aagatattca gcaatcggtg actgaagtta
     3361 aaaaccatct cgatttgttg gggcaagtct tttacgactt tgacagtcgg gattatttta
     3421 gtggtgagcc acaagcgcaa ttatcctgcc tcaaccgcgc ggcggaattc gttctgcgta
     3481 cccagaaagt tgaacgtcgt tttatgggac tggttaaacg catgaaagcc gcctacgacg
     3541 tctgctgcgg cagtgaagca ctatcacaga cagaacgtga tcatattcac tattatcttg
     3601 ctgttcgttc aattgttttc aaactgacga aaggtgacgc accggatgtt acccagatga
     3661 atgcacgcgt tcgtgaaatg attgcagaag cgctaaaagc tgatggcgta gaagaaattt
     3721 attttcttgg cgataaaaaa gcggaatcca tcgatatttt tgacgaagat tatctggcgc
     3781 gaattaacaa gatcaaactt ccggcaacga agatccagct attacaaaaa ttactggaaa
     3841 aagcgatcag cgacttcagg aaagtgaacc agttgcaagg gattaacttc acccgccgct
     3901 tccaggctat tatagatcgt tataatgagc ggcgagaaga tgatgtactc aacggtgaag
     3961 aattcgatac attcagtcag gaaatgaccg atattatcta tgatattaaa acagaaatgg
     4021 gcacctgggc cgatttaggt attgatattg aagaaaaagc gttcttcgac attcttgctc
     4081 atatgcgcga taaatatcag ttcacctatg acgatgaaaa aatgctgtcg ctggcaaaag
     4141 agatgaaaag cgtggttgac aacacatcga aatatcctga ctggagtaaa cgcgatgata
     4201 ttaaagcgaa actgaaagtt gaacttattc tgcttctaca caagcataag ttcccgccag
     4261 tagcgaatga tgatgtttat atgggggtac tggcgcaagc agagaacttt aagaaaaatc
     4321 acatgagttg agtctgtcat aatggagtat ctcatcagat actccttctt tatctatttt
     4381 gtaagagcca aaatagataa attat
//
LOCUS       DAZFKF010000218         4314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFKF010000218 DAZFKF010000000
VERSION     DAZFKF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.220"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            360..968
                     /locus_tag="ACHKA1_005239"
     CDS             360..968
                     /locus_tag="ACHKA1_005239"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO5682756.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1066..2457
                     /gene="selA"
                     /locus_tag="ACHKA1_005240"
     CDS             1066..2457
                     /gene="selA"
                     /locus_tag="ACHKA1_005240"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO5682757.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2454..4298
                     /gene="selB"
                     /locus_tag="ACHKA1_005241"
     CDS             2454..4298
                     /gene="selB"
                     /locus_tag="ACHKA1_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO5682758.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDIHSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          964 a         1098 c         1275 g          977 t
ORIGIN      
        1 gtcactccgc cggggcacac cgaacaggcc acgccggtgg gggcaccgat gcgcaccccg
       61 gctgaaccct ggacaatgct accgccatac tgcgtcatgt cgccctgacg cgccgccggt
      121 tttccgctca tctgctcatc cttgtattat cgggttattt gtccgctgac aggcaaaacc
      181 ctacagattt ataagaaagc cttaagtgag atatctgtca gataagaaca attaggaaaa
      241 tatttatatc accatgataa atatgtgatt tattgggagg tgacaaacca tcaacatttt
      301 ctgccctttt tcccatcacc aggccatact gatatttttc ctcgcacaaa ggactcggca
      361 tgaaactcgt cggtagctac accagcccgt ttgtacgcaa actttctatt ctgttgttag
      421 aaaagggcat tactttcgaa tttattaatg aactgcccta taacgcggac aatggcgtgg
      481 cgcaatttaa cccgttaggg aaagtgccag tgctggtgac cgaagagggc gaatgctggt
      541 ttgattcgcc gatcatcgcc gaatatattg aattaatgaa tgtcgctccg gcgatgttgc
      601 cgcgcgatcc tttggagtcg ttgcgggtgc gcaaaattga ggcgctggcc gacggcatta
      661 tggatgctgg gctggtgtcg gtgcgtgaac aggcgcgtcc ggcggcgcag cagtctgaag
      721 atgaattgtt acgccagcgg gagaaaatca accgcagtct ggatgtgctg gaaggatatc
      781 tggtcgatgg cacacttaaa accgatacgg tcaatctggc gactatcgcc attgcctgtg
      841 ccgtcggata tctcaatttc cgccgcgttg cgccaggctg gtgtgtcgac cgcccacact
      901 tagtcaaact ggtcgaaaac ctgtttagcc gcgaaagttt tgctcgcacc gaaccgccaa
      961 aggcttgatg cgcgatatgt cctcctgacc catctcacgt tacaatccgt ggttatgtta
     1021 aacgcccttc tccgtgtgag agggccttga tcagccaggt ttcctatgac aaccgaaacg
     1081 cgttccctct atagtcaact tccggctatt gatcgcttat tgcgcgatag ctccttcctt
     1141 tctttgcgtg atacttatgg tcacacccgc gtggtggaat tgctgcgtca gatgcttgac
     1201 gaagcgcgag aagtgattcg tggcagccag acgctgcctg cgtggtgtga aaactgggcg
     1261 caagaagtcg atgcccggtt gacgaaagag gcgcagagcg cgctgcgtcc ggtgataaac
     1321 ctgacgggaa ccgtgctgca taccaatctt gggcgagctt tacaggcgga agctgcggtg
     1381 gaagccgttg cgcaggctat gcgttcgcca gtgaccctcg agtacgatct ggacgacgcc
     1441 ggacgcggac atcgcgatcg ggcgctggcg cagctgctgt gccgcattac cggcgcggaa
     1501 gatgcctgta tcgttaataa caatgcggcg gcggtgttat tgatgttggc ggccactgcc
     1561 agcgggaaag aggtggtggt ttctcgcggc gaattggtgg agattggcgg cgcgtttcgt
     1621 attcccgatg ttatgcgtca ggcaggctgc acactacacg aagtagggac caccaaccgc
     1681 acgcacgcga atgattatcg tcaggcggtg aatgaaaata ccgcactgtt gatgaaagta
     1741 cataccagta actacagcat tcaggggttc accaaagcga tagacgaagc tgaactggtg
     1801 gcgctcggca aagagctgga tgttccagtg gtgactgatt taggcagtgg ctcgctggtc
     1861 gatcttagcc agtacggttt gccgaaagag ccaatgccgc aggagttgat tgcggcgggc
     1921 gtcagtctgg tgagtttctc cggcgacaag ttgttaggcg ggccgcaggc aggaattatt
     1981 gttggtaaaa aagagatgat cgcccgcctg caaagccacc cgctgaagcg tgcattacgc
     2041 gcggataaaa tgacccttgc ggcgctggaa gccacgttgc gtctttattt acaccctgaa
     2101 gctctgagtg aaaaattacc gaccctgcgc ctgcttaccc gcagcgcaga ggtcattcaa
     2161 atccaggcac aacgtttaca ggctcccctt gccgcacatt acggcgcgga gtttgcggta
     2221 caggttatgc catgtctttc gcagattggc agtggttcgc tgccggttga tcgcctgccg
     2281 agcgcggcat taacgtttac accccatgat ggacgcggta gccaccttga gtcattagcc
     2341 gcccgctggc gtgaattgcc agtgccggtg attggtcgta tttatgacgg acgattgtgg
     2401 ctggatttac gctgccttga agatgagcaa cggtttttgg agatgttgtt gaaatgatta
     2461 ttgcgactgc cggacacgtt gaccacggca aaacaacctt attgcaggcg attactggcg
     2521 taaatgccga tcgtctgccg gaagaaaaaa agcgcggcat gaccatcgat ctcggctatg
     2581 cctactggcc gcagccggat ggtcgtgtgc ctggttttat cgacgtaccc ggtcatgaaa
     2641 agtttctttc caacatgctg gcgggcgttg gtggtatcga tcacgcgctg ttggtggtgg
     2701 cgtgcgatga cggcgtgatg gcacagaccc gtgagcatct ggcgattttg cagctgaccg
     2761 gtaacccgat gctgacagtg gcgctgacca aagccgatcg cgtggacgaa gcgcgtgtta
     2821 atgaggttga acgccaggta aaggaggtgc tgcgggaata cggttttgct gaggcaaaac
     2881 tgtttatcac cgcagcaacc gaaggtcggg gaattgatgc cctgcgcgag catctgcttc
     2941 agttaccaga acgtgaacac gccagccaac atagtttccg cctcgcgatc gaccgcgcat
     3001 ttaccgtaaa aggtgccggg ctggtcgtca ccggtacggc gttaagcggg gaagtgaagg
     3061 taggcgattc actatggctg actggtgtaa ataaaccgat gcgcgtgcgt gcgctgcatg
     3121 cgcaaaacca gccaacagaa accgctcatg ccgggcagcg tatcgcgctt aacatcgcgg
     3181 gggatgcgga aaaagagcag attaaccgtg gcgactggct gcttgccgat gtgccgccag
     3241 agccgttcac gcgggtgatt gtcgagcttc aaacccatac gccgctgacc cagtggcagc
     3301 cgctgcatat tcaccacgcc gccagccacg ttacgggacg cgtttcgctg ctggaagata
     3361 accttgccga gctggtgttc gacacgccgc tgtggctggc ggataacgat cgcttagtgt
     3421 tgcgcgatat ctcggcccgc aatacgctgg cgggggcgcg tgtggtgatg cttaacccgc
     3481 cgcgtcgcgg taaacgtaag ccggaatatc tgcaatggct ggcgtctctt gcacgggcgc
     3541 agagcgatgc cgatgcgtta tctgttcatc tggaacgcgg agcggttaac cttgcggatt
     3601 tcgcctgggc gcgccagctc aacggcgaag ggatgcgcga actgctgcaa cagccgggct
     3661 atattcaggc tggttatagc ttgttgaatg cgccggttgc cgcccgctgg cagcggaaaa
     3721 ttcttgacac attagcgact tatcatgagc aacatcgcga tgaacctggt cctgggcgcg
     3781 aacgtctgcg acgtatggcg ttgccaatgg aagatgaagc gctggtactg ttgctgattg
     3841 aaaagatgcg cgaaagcggc gacatccaca gccatcacgg ctggttgcat ctgccagatc
     3901 acaaagcggg cttcagcgaa gagcagcagg ccatctggca aaaagcagag ccgctgtttg
     3961 gtgacgagcc gtggtgggtg cgtgacctgg caaaagagac gggaaccgac gagcaggcaa
     4021 tgcgtctgac gctacgccag gcggcccaac aggggataat taccgcgatc gtcaaagatc
     4081 gttattaccg taacgatcgg attgtcgagt ttgccaatat gatccgcgat ctcgatcagg
     4141 aatgtggttc aacctgcgcg gcggatttcc gcgatcgctt aggggttggg cgaaaactgg
     4201 cgattcaaat cctggaatat tttgaccgca ttggctttac gcgtcgtcgt ggaaatgatc
     4261 atttattgcg cgacgcatta ttatttccgg aaaaataagg aaatgattaa ttaa
//
LOCUS       DAZFKF010000219         4205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFKF010000219 DAZFKF010000000
VERSION     DAZFKF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.221"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            136..462
                     /locus_tag="ACHKA1_005242"
     CDS             136..462
                     /locus_tag="ACHKA1_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO5682759.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(594..794)
                     /locus_tag="ACHKA1_005243"
     CDS             complement(594..794)
                     /locus_tag="ACHKA1_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5682760.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            836..1201
                     /locus_tag="ACHKA1_005244"
     CDS             836..1201
                     /locus_tag="ACHKA1_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5682761.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            1484..2041
                     /locus_tag="ACHKA1_005245"
     CDS             1484..2041
                     /locus_tag="ACHKA1_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5682762.1"
                     /translation="MLTTQKRKFALALMSGKNRTAAAIEAGYSEKSARCKGSQLAKDP
                     EVLAFIARKQGETVEVDEVPVYRQKKTQACHREVPPVEKKNQVSVPPDDGQSFSPLSG
                     IDYMEDGLPDPVKAMGRILVENISIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGDAA
                     KKAANRFAVPPPPRLVVNNDNEGNG"
     gene            2038..3699
                     /locus_tag="ACHKA1_005246"
     CDS             2038..3699
                     /locus_tag="ACHKA1_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO5682763.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKINDPHFLPVIFEHPPEMVERGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVASGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQSWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            3763..>4205
                     /locus_tag="ACHKA1_005247"
     CDS             3763..>4205
                     /locus_tag="ACHKA1_005247"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO5682764.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGVHFLRWELMEVSAVTVPANAECT"
BASE COUNT          933 a          997 c         1280 g          995 t
ORIGIN      
        1 gctaaatgcc gcatggtatc cgctgcatta cagccagtgt gttgagcgac cttattatcc
       61 atgcgcggta ttgtgatggc cgacagggtg gcattgttag aataagactt attcttatct
      121 gtgccgggaa tgaaaatgaa aagaaatctt ccgttaatta ttttgttgtc ttctctggtt
      181 atgggctgta cgcaacataa aacagatatg ccccgacagt tggttaaggc attaccacaa
      241 tatccggcct atgcagcggc aaattatata aagggacggg ttgatgtgaa gtttgatatt
      301 ggtgctgatg gtactgtcac ccgaattgag tttatccgtt cagagccgca ccatctgttt
      361 gatgagcagg ttgtaaaggc gatggcaaaa tggcgatttg agaaggacag gccgcgtaaa
      421 ggcgtgaaga aaacgtttat ctttagtcct tctgcaccct gattatttca tcagaaatta
      481 attatcactc tgttgttatt ctgtacatcc cggctgggta agtcttgttc cgccgggtat
      541 gaagatgaaa tattgttgga ggacagtggg tacctgctcc tgtaaccgaa cgttcatttc
      601 tcgttatttg tcatgctggc cgggcgcagg tgcgttgcat ctgttgccag ccttctccgg
      661 caggcttcaa taacccacgc tgaaaagtta ccggaccctt tatgctcaag ggcgatgttg
      721 atctgttcaa tcatgtgatt ggggaaacgg atattgcggg ttgtggttct gcgggtccgg
      781 tttttcgatg acatatttat ttcctttact gattgcctta tgacggggat tttacatggc
      841 tgagcttcgt acactccaga gcagaatcaa aacactgaat acccgacggg tgaatattct
      901 gaagggtgaa cagcgtcgtg tcagtggcag tgcacgtgtt tccctcaagc gtcatatctg
      961 gctcagggat gccgggcagt gctgtctctg tggtcgtgtg gttgacctct gtgacagtga
     1021 actcgatcac cgaattgcac ttcagttcgg tggtggtaat gaggagacga atctctggac
     1081 gctctgtacc gaatgccatc gacaaaagtc tgctcgtgaa gcggcgggtg gtatgccgga
     1141 cccgacactg ccggaggtgt ccggaggtag tggcagagcg gacgacatca tcggactgta
     1201 acccgacccg gggggtatca tccggcgtaa aaaacgatcg ctccggacac cgcgccccct
     1261 ctcacgcaga gaaaaaattc cccttcagaa gttataaaca tgtttataac ctactgatgg
     1321 atttttaccg tttttttctt tttattcata acgttgtggg aggtaaatgt ttataccttt
     1381 ttttcgcaaa atgtttacag ggcagcggta aacattttcc ctgccgcctc cattcttacc
     1441 gggaggattg ctgacgtttt tctgtttttc cccggaggaa agaatgctga cgacgcaaaa
     1501 aagaaaattt gcgctggcgc tcatgtcggg caaaaacaga acggctgctg ctattgaggc
     1561 tggttactcg gaaaagtccg ccagatgtaa aggctcacaa ctggcaaaag atccggaagt
     1621 gctcgcgttt atagcccgta agcagggcga gacggtggag gtggatgagg ttcctgttta
     1681 ccggcagaaa aagacgcagg cgtgccaccg ggaagttcct ccggtggaaa agaaaaacca
     1741 ggtttcagtg ccaccggatg acgggcaaag tttctctcca ctatccggta ttgattacat
     1801 ggaggacggc cttcctgatc ctgtgaaagc catgggtcgg atcctggtgg aaaacatcag
     1861 cattgatccg aaactggctc tggatgcagc ctggcgactg gcgcagttca cgcaccacaa
     1921 aaaaggggat gccggtaaaa aatcggcaaa aggtgacgcg gcgaaaaaag cggctaaccg
     1981 ttttgcggtg ccaccaccac cccgcctggt ggtgaataat gataatgagg gcaacggatg
     2041 atacctgtgt ggagcacggc ctgcccggac tgggcagagc gtctgaaaaa ggggctgtcg
     2101 attattccgg ctccgattta cccggaccag gccgcacatg ccctggcgat ttttaaacaa
     2161 ctgcggattg tggatgcacc gggcagcccg acgttcgggg agtcctgtgc accgtgggtg
     2221 tttgatctgg tggctgccct gtttggctcc tacgatgcgc agaccggtgt acgccatata
     2281 aaggaagtgt tcattctgat acccaaaaaa aacagcaagt ccacgctggc tgccgggatc
     2341 atgatgacgg cgctgttact gaactggcgg caggcggcgg gctacaccat tctggccccg
     2401 accgtggagg tggcggctaa cgccttcaac cctgccaggg atatggtacg acgggacgat
     2461 gatctggatg acctctgtca ggtgcagaca catatccgga ccatcaccca tcgggtgacg
     2521 gacaccaccc ttaaggtggt ggctgccgat ccgaatacgg tgtccggtat caagtccgtg
     2581 gggacgctga ttgatgaact gtggttattt ggcaagcagt acaaagcgga ggacatgtta
     2641 cgtgaagcca tcggcggcct tgcctcccgc ccggaagggt ttgtggtgta cacaaccacc
     2701 cagtcgaatg agccgcccgc cggggtgttc agacagaaac tgcagtacgc ccgggatgtg
     2761 cgcgacggca aaattaatga tccgcacttt ctgccggtga tatttgagca tccgccggag
     2821 atggtggagc gcggtgagca cctgctgatg gaaaacctcg ccatggttaa cccgaatctc
     2881 ggttattcgg tggatgaggc ttttctgtac cgggagtacc gtaaagcccg ggaggctggt
     2941 gaggaggcat ttcgtggctt catgtcaaaa cacgccaatg tggaaattgg tcttgccctg
     3001 cgttctgacc gctgggcggg tgcggatttc tgggagcagc agggcaggcg cgtcagcctg
     3061 gacgatatcc tgcagcgcgc tgatgtggtg acggtgggga ttgacggcgg gggcctggat
     3121 gatctgctgg gaatgtacgt gattggtcgt gacagggaaa cccgcgaatg gctgggctgg
     3181 ggccatgcct gggcgcatga aaccgcggtg gtcagacgga agagtgaggc atcccggttt
     3241 caggattttg tggccagtgg agacatgacg attgtccgtc gggtcgggga tgacacggcg
     3301 gaagtggcgg agtatgtgcg tcgtattcat gaggctgagt tactggacca tatcggtatt
     3361 gacccgtcag gtgtggggca gattctggat tcactggcag aagccgggat ccccgacgga
     3421 attgttgtgg ggataagcca gagctggaag ctgggcgggg ccatcaaaac caccgagcgc
     3481 aaactggctg agggagtgct ggtgcatggt ggtcagccac tgatggcctg gtgcgttggc
     3541 aatgcccggg tggagcctaa aggtaacgcc atccttatta ccaaacaggc cagcggacgg
     3601 gggaaaattg acccgctgat ggcgctgttc aatgcggtat ccctgatgtc cctgaatccg
     3661 gagcccaaaa agaaagaata tgcggttttt ttcatataac cctgttcacc ctgtaaccat
     3721 catggaccgc tgcggcggtt tttttatttt caggaggctg atgtgactct taaacgggcc
     3781 tgctccctgc tgacggtgaa atccttcagt gaggatgagc gggtgatcac cgggattgcg
     3841 tcaacgcctt ctccggatcg ggatggtgac atcctggagc cggagggcgc ggagtttggc
     3901 agtgcgatcc cgtttctctg gcagcatgac cattcccgcc cggtggggca gtgtacggtg
     3961 cgccgggtca gcgaagggct ggaaatcacg gcaacactgg tgaaacccgt accggatatg
     4021 ccgtcgcaac tggctgcccg gctggatgag gtctgggcgg ccattaagac cgggctggtc
     4081 agggggctgt ccgtgggctt ccgtcctcat gaatacacct ttctggacgg aggcggagtg
     4141 cattttctgc gctgggagct gatggaggtg tctgccgtca ccgtgcccgc gaatgcggaa
     4201 tgcac
//
LOCUS       DAZFKF010000220         4119 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFKF010000220 DAZFKF010000000
VERSION     DAZFKF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.222"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1093
                     /locus_tag="ACHKA1_005248"
     CDS             <1..1093
                     /locus_tag="ACHKA1_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO5682765.1"
                     /translation="KLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKHYF
                     SSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAG
                     LKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDF
                     FGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRTDT
                     QVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVDYK
                     INLLDKNDFTKALGVSTDDIVAVGLVYQF"
     gene            complement(1283..1666)
                     /gene="quuD"
                     /locus_tag="ACHKA1_005249"
     CDS             complement(1283..1666)
                     /gene="quuD"
                     /locus_tag="ACHKA1_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO5682766.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDVVVNNSN"
     gene            complement(1752..1892)
                     /locus_tag="ACHKA1_005250"
     CDS             complement(1752..1892)
                     /locus_tag="ACHKA1_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO5682767.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGYVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(1889..2251)
                     /gene="rusA"
                     /locus_tag="ACHKA1_005251"
     CDS             complement(1889..2251)
                     /gene="rusA"
                     /locus_tag="ACHKA1_005251"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO5682768.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2248..2538)
                     /locus_tag="ACHKA1_005252"
     CDS             complement(2248..2538)
                     /locus_tag="ACHKA1_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO5682769.1"
                     /translation="MVDLRKAARGRECQVRIPGICNGNPETSVLAHIRLAGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAGYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2531..2701)
                     /locus_tag="ACHKA1_005253"
     CDS             complement(2531..2701)
                     /locus_tag="ACHKA1_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO5682770.1"
                     /translation="MTTPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2701..3156)
                     /gene="ybcN"
                     /locus_tag="ACHKA1_005254"
     CDS             complement(2701..3156)
                     /gene="ybcN"
                     /locus_tag="ACHKA1_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO5682771.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQIQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3153..3251)
                     /locus_tag="ACHKA1_005255"
     CDS             complement(3153..3251)
                     /locus_tag="ACHKA1_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682772.1"
                     /translation="MRPQVELLALPQMQKCTCVFLHPTFDLCGGEA"
     gene            complement(3327..3578)
                     /locus_tag="ACHKA1_005256"
     CDS             complement(3327..3578)
                     /locus_tag="ACHKA1_005256"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682773.1"
                     /translation="MMSFTKCKLMHSHKTSVNAALLDDTKLVVERDLHFLRELYISSP
                     EEFVGHWVYANEPIFHEHPLSVGLSGIVYHKVIDEIEKS"
     gene            complement(3638..3967)
                     /locus_tag="ACHKA1_005257"
     CDS             complement(3638..3967)
                     /locus_tag="ACHKA1_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFO5682774.1"
                     /translation="MDAGVLVLAVQQSPITKQFTDNELCTLAWLWRAGNVMLIAYQNV
                     THLLQDAEHGEAGHFTSIEQEYPRILNRARAILVRETAHVKLQPWQDDKWSRVLPHLR
                     QNVIKQR"
BASE COUNT         1093 a          983 c          900 g         1143 t
ORIGIN      
        1 aaaattaaca gtggcaattt ctgctgtagc tgcatcagta ctgatggcga tgtctgctca
       61 ggcagctgaa atttataata aagacagtaa caagctggat ctgtacggga aagttaatgc
      121 caagcactac ttttcctcta acgatgcaga tgatggtgat actacttatg ttcgtctggg
      181 cttcaaaggc gaaacccaaa tcaacgatca gctgactggt ttcggtcagt gggaatatga
      241 attcaaaggc aaccgcgctg aatctcaagg ttcctccaaa gacaaaaccc gtcttgcatt
      301 tgcaggcctg aaattcggtg actacggctc aatcgattac ggccgtaact acggtgtagc
      361 atacgacatc ggtgcgtgga ctgacgttct gccagaattc ggtggcgata cctggaccca
      421 aacagatgtg ttcatgactg gtcgcaccac tggtgttgca acctatcgta acaacgactt
      481 ctttggtctg gttgatggtc tgaactttgc tgctcagtac caaggcaaaa acgatcgtag
      541 cgatttcgat aactacaccg aaggtaacgg tgatggcttc ggtttctctg ctacctatga
      601 atacgaaggg ttcggtatcg gtgcaactta tgcgaaatct gatcgtaccg acactcaagt
      661 taatgcaggg aaagttcttc ctgaagtatt tgcttccggt aaaaatgcag aagtttgggc
      721 cgcaggtctg aaatatgacg ctaacaacat ttacctggcc actacctatt ctgaaaccca
      781 gaatatgact gtatttgctg atcacttcgt tgctaataaa gctcaaaact tcgaagctgt
      841 tgcacaatat cagttcgatt tcggtctgcg tccgtccgtt gcttacctgc aatctaaagg
      901 taaggatctt ggagtatggg gcgatcagga cttagtcaaa tatgttgatg taggtgcaac
      961 ctattacttc aacaaaaata tgtctacttt cgttgattac aaaatcaacc tgcttgacaa
     1021 aaatgacttc actaaagcac tcggtgtaag cactgatgac atcgttgctg taggtctggt
     1081 ttaccagttc taatctgatt acgaaaaaga tatgttgcgg gaggctttgc ctccccaaca
     1141 tataagtggc tccctcaagc cacttccttt aggagcacaa ccttgcttct aactatataa
     1201 accttctgtt atatattacc ctttattttt gggggcgttg caacgcccca tttttaataa
     1261 tttttagtaa acaattggca tattaattag agttattaac aacgacatcc atctctaacc
     1321 ggatatctaa tgccattaac atcccttcaa ttatgccctc agccttctgt aaccttttcc
     1381 cgatataacc atcagagcag caatgcttac ctgccagtga catgaatgtc ataccgacta
     1441 cataataatc tactaataaa tcgtgcaaat cgctgttgtt ctttttcaga cgggccatgc
     1501 acccgcaaat gatcatcgcg tcatcgtcac aacattgcgg gcgagatttt acttttgaag
     1561 gaattaatcc cttaaaaccg gcggcaatgg acgaccaggt cacatcttca tgattattag
     1621 ccgcccacgc tccccaacgc tcaagaacca tctgaatatc acgcatcaac ttactccaca
     1681 aaactcagac cagaacgcca attacaagca aaaatcaaca aaacagtatt agttgattgt
     1741 tatctctgac ttcatactcc tgctcctgtc agtgttttgg cgtaattctt cagtattcgg
     1801 taatcggtca aaacataacc ggggaaacga tataagcgca gacgccccca gcggtggcga
     1861 agaagttctg ccatattaaa ctcaaacatc attcattccc catttcggtg atggtcagtt
     1921 ccagcctccc acctttggta acaggcatct tcacaacgcg gtaatcaacg acctgagcat
     1981 catccagcca gaaacctgct ttagtgagtg cgtcaaaagc ggctttttgc agattatcca
     2041 ggtcacggcg acggcgatcc ggcatgtggc actcaatgcg gattttcaca ggcatagcca
     2101 ggccgatatc cagcattgcg tttttaatga ttcgggcgac gttatcgcgg tatgcctgcc
     2161 cctctgcgct gatgtgcgtg cgcccgcgat tatggcggta atagcgatta ttgctcggag
     2221 gccagggtaa tgtgatgctg taggtattca cgccttaata accccctctt tcagccagat
     2281 aacctgtgtt ctcgccatac cttccagcgc gcattctttt gcatatccag cgtcaacaaa
     2341 atgcgtgcga cggtcgatct catcgtgaca ggcagaacat gcaatggtgg caatcaggtc
     2401 tggcggtttg gtaccggtgc cacacaatcc agccagccgg atatgtgcca gtacagacgt
     2461 ttcagggttg ccattacata tgccagggat tcttacctgg cattcccgac cacgcgctgc
     2521 ttttctcaaa tcaaccatga ttcctccttg ctgccagtcg caaccatttt ttatcaacca
     2581 ggctggcggt ataccctagc agtgttggta tttcggatgg cttcagctca ggcttacgct
     2641 tacgacgatt tggtactctg tagatgtgtc cgttcatgac acgaataagc ggtgtagtca
     2701 ttacgcctcc tgcttgtcgc gcagcagctg gaactcgcag ctctgtggaa tagtcaggtg
     2761 gcaaccaata ttcatcgccc aggcttcaac cttacacagg aagacataca tctctccggt
     2821 atcaagatcg gaggtatggc gtaacgactg gatcgtagtg atttcgccgg ttacgacatc
     2881 aaccaggtcc ttggtttcat aaccgaggta tgtgtgtttg agagcatctt ttacccatgc
     2941 tgcagtagcg aacgatttcc ccctgctgat gaggtattca ctgatttcgc tgtaccacat
     3001 gtggctgagt gcattctggg aaagactgcg tctttcgcgc cacggtttaa gcaccatgcg
     3061 aaagcatttg ccgtcctcca gataaggctg gatctgctga ccgatagcgg tgaagttacc
     3121 gcgatgcaat ttgataccgt cttgtgggag gttcacgctt cacctccgca gaggtcaaac
     3181 gttggatgca aaaaaacgca ggtgcatttc tgcatctgtg gcaaggcgag gagttcaact
     3241 tgtggtcgca tataatttcc caatcatatg cgcagaagtc accgcagggt gttcaggcca
     3301 acggtgacgg tattattgct tattgattat gatttttcaa tttcatcaat aaccttgtga
     3361 tagacaatac cagaaaggcc aacagacagt ggatgttcat gaaatattgg ctcatttgca
     3421 taaacccaat gcccgacaaa ttcttctggg cttgatatgt ataactcacg aagaaaatgg
     3481 agatccctct ctacaactaa tttcgtatcg tccaagagcg ctgcatttac gctagtttta
     3541 tggctgtgca ttaatttaca tttggtgaag gacatcatgg caaaagctcc tttttcttat
     3601 gaatccatat gttagctaaa ttggagagac acagtagcta acgttgttta ataacattct
     3661 gacgcaaatg tggcaatact cgactccact tatcatcctg ccacggctga agttttacat
     3721 gtgccgtttc tcggacgagg attgctcgcg ctctgttgag tatccgggga tattcttgct
     3781 cgatggaagt gaagtgacca gcttcaccat gctccgcatc ctgaagaaga tgagtaacgt
     3841 tctggtaggc aattaacatc acattccctg ctcgccataa ccaggcgagt gtgcaaagtt
     3901 cgttatcagt gaattgtttt gtgattgggg attgttgaac tgctagaacg agaacgccag
     3961 catccattgg cagtccctat agtaaaacca tagctcagga cgcttcgttc aggatagata
     4021 attttattgt acttacctaa ctttcttact atagcacggt tgaaaaagtg attattactc
     4081 aaaaataaac ctcaccatca accatatatt tgagagtac
//
LOCUS       DAZFKF010000221         4097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFKF010000221 DAZFKF010000000
VERSION     DAZFKF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.223"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..948)
                     /locus_tag="ACHKA1_005258"
     CDS             complement(<1..948)
                     /locus_tag="ACHKA1_005258"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO5682775.1"
                     /translation="MTNSRIPDERQFLTSDNTELFYRHWPAQEASSTPKVVVLFHRGH
                     EHSGRLQHIVDELAMPDTAFYAMDARGHGRSPGLRGYSPSLVRSVQDVDEFVRFAAAD
                     SQAGMTDVVVVAQSVGAVLAATWAHDYAPDIRGLVLASPAFKVKLYVPFARQGLELMY
                     RLRGLFFVNSYVKGKYLTHDPERVASFNKDPRITRPIAVNILLDLYKTAERVVSDASA
                     ITLPVQLLVSGEDYVVHRQPQLDFYQNLRNPQKELHVLPGFYHDTLGEKERHLAFDRM
                     RSFIDTLYARPVQRFDYSQEDRWSPSADTWRILSGGPALY"
     gene            complement(941..1582)
                     /locus_tag="ACHKA1_005259"
     CDS             complement(941..1582)
                     /locus_tag="ACHKA1_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO5682776.1"
                     /translation="MMTRLLRTLFTLCIVWPVIWLWLGLRVRHRERLPKHGPSIIVAN
                     HNSHMDVFALLSLFPLRYQASVHPVAAADYFLRDKRMAWFALKILNIIPISRKGGYTD
                     PLAQCEQALREGKTLILFPEGTRGEPGVLAPLKSGVWHLSQRMPEVPIIPVGLRGTER
                     VMAKGNRIPLPLFIDVNIGDALSTHPEKKTFMDDLEQRLTALLQPSQGNHPHD"
     gene            complement(1586..2503)
                     /locus_tag="ACHKA1_005260"
     CDS             complement(1586..2503)
                     /locus_tag="ACHKA1_005260"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO5682777.1"
                     /translation="MTLLEKSLIVIFSLLLIATVINGLLVWLRPSKDWRELTLRIRTW
                     WVIIVLFSFALLSPSWLALTFFALVSFMALKEFLTLVPSRQSDRMPLLWMFIAIPLNY
                     WLIGIGWYGMFVVFIPVYVFLFLPARMVIAGDTQGFLRTVSQLHWSLMTTVFAFSHVA
                     FLLVLPADGMQTSALLVLFLVGLTEFNDIAQYLWGKSLGRIKVIPKVSPNKTLEGLLG
                     GVATTALAATVLGPLLTPLSWPMALLAGVIIGVTGFCGDVVMSAIKRDIGVKDSGTLL
                     PGHGGILDRLDSLIFTAPVFFHFVRYFCY"
     gene            complement(2500..3105)
                     /locus_tag="ACHKA1_005261"
     CDS             complement(2500..3105)
                     /locus_tag="ACHKA1_005261"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO5682778.1"
                     /translation="MTLYQIKPSFQALLRPLMFWLNRRGVTANHITLCAMVLSLITGV
                     VLIIHPQPGLFVLLPVVLFVRMALNALDGMLARECNQRSRLGAILNETGDVISDISLY
                     LPFMLLPQSSIPLVLIVVFCALMTEFCGVLAQTINGVRSYAGPLGKSDRALVFGAWGL
                     SLSIWPQIVAWNNVIWGVATLLLLWTIFNRCRSALREEGVK"
     gene            complement(3163..3348)
                     /locus_tag="ACHKA1_005262"
     CDS             complement(3163..3348)
                     /locus_tag="ACHKA1_005262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682779.1"
                     /translation="MLTQLHRVLYRCPGHYVYGSATQSTGTGTGSLASRNTSQSPLPA
                     QQAGFPRPELYSLSVCQ"
     gene            complement(3589..>4097)
                     /locus_tag="ACHKA1_005263"
     CDS             complement(3589..>4097)
                     /locus_tag="ACHKA1_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5682780.1"
                     /translation="HFILLLTGAMAGSIMVALPDINDRIDNITGYILIVAGVLSASFY
                     VVISSRLIVNFEPVTLLTGQLTFSFILACFFIVLTGTSLQLPIESVFLVFLSGILQYF
                     LAFCFYLYSLRWIPVHIAGAMLYFIPLVALLLSWFFLQEDISFIQGLGVFITILCVYI
                     LNKKYAIE"
BASE COUNT         1158 a         1085 c          991 g          863 t
ORIGIN      
        1 atacagcgca gggccaccgc tcagtatgcg ccaggtatcg gcgctggggc tccagcgatc
       61 ttcctggctg taatcaaaac gctgcacggg acgggcatac aaggtatcaa taaaactgcg
      121 catcctgtca aatgccagat gccgctcttt ttcgcctaag gtgtcatgat aaaatcccgg
      181 cagtacatgc agttctttct gcggatttcg caggttctga taaaaatcaa gctgcggctg
      241 gcggtgcaca acgtaatctt ccccggagac cagcaactgc acaggtagcg taatggcgct
      301 ggcatcgctg acgactcgct cagccgtttt gtagagatca agcagaatat tcacagcaat
      361 tggtcgggta atccgcggat ccttattgaa gcttgctacc cgctcgggat cgtgcgtcag
      421 atacttgcct ttcacatacg agttgacgaa gaaaagaccg cgcaggcgat acatcaattc
      481 cagcccctgg cgagcaaacg gcacatacaa tttgacctta aacgccggtg atgccaacac
      541 caggccacga atgtccgggg cataatcatg cgcccaggtt gccgccagaa ccgccccgac
      601 gctttgcgcc acgacgacca catccgtcat cccagcttgg ctatctgcgg cggcgaaacg
      661 cacaaactcg tcaacatcct gcaccgaacg cacgagcgac ggactgtaac cacgcagacc
      721 cggtgagcga ccatgacctc gggcatccat ggcataaaac gctgtatccg gcatcgccag
      781 ctcatcaaca atgtgttgca aacgcccgga gtgctcatgc ccacgatgaa acaggaccac
      841 cacttttggc gttgaactgg cttcttgtgc tggccagtgg cgataaaaca gctctgtatt
      901 atcactggtc agaaactgcc gttcatcggg tatgcgggaa ttagtcatgg ggatgatttc
      961 cttgcgaggg ttgcaaaagc gccgttagtc tttgctccag gtcgtccata aacgttttct
     1021 tctccggatg cgtacttagc gcatcgccaa tatttacatc aataaacagc ggcaaaggga
     1081 tacgattacc tttcgccatc acacgctcag taccgcgcaa cccaacgggg ataatgggca
     1141 cctccggcat gcgctgactc aaatgccaca ctccggattt caatggcgca agtacgcctg
     1201 gctcaccgcg cgtcccttcc ggaaacagga tcagtgtttt accttcacgc agcgcctgtt
     1261 cacattgcgc cagcggatcg gtataaccgc cctttcttga aataggaatg atgttaagga
     1321 tcttcagcgc aaaccaagcc atgcgtttat cccgcagaaa ataatccgcg gcagcaaccg
     1381 gatgaacaga agcctggtaa cgcagcggaa aaagcgaaag caaggcgaaa acatccatat
     1441 ggctgttgtg atttgccacg attatcgaag gcccgtgctt cggcagtcgc tcgcggtggc
     1501 ggacacgcag accaagccac agccagatga ccggccagac gatacacagc gtaaacaggg
     1561 tacggagtag tcgtgtcatc ataggttagt agcagaaata gcgaacaaaa tggaagaaga
     1621 ccggtgcggt aaaaatcagc gaatccagcc gatcaaggat gcctccatgg ccgggtagca
     1681 gcgtaccact gtctttgacg ccgatatcac gcttgattgc cgacatcacc acgtcgccgc
     1741 aaaagccagt aacgccgata ataaccccgg ctaacagtgc catcggccag ctcaggggag
     1801 tcaacagtgg ccccaggact gttgccgcca gcgccgtcgt tgccacccca cccagcaatc
     1861 cctccagggt tttgtttggg ctgaccttcg gtatgacttt tatacgacca agcgacttgc
     1921 cccacaggta ttgcgcgatg tcgttaaatt cggtcaggcc gaccaaaaac agcaccaaca
     1981 gcgcgctggt ttgcattcca tccgccggta acaccagtaa aaacgcgaca tggctgaagg
     2041 caaatacggt ggtcatcaga ctccagtgca actgggaaac ggtacgtaaa aagccctgtg
     2101 tatccccagc aatcaccatt cgcgccggta aaaaaaggaa cacgtagacc gggataaaca
     2161 ccacaaacat gccgtaccag ccgataccta tcagccagta gttaagggga attgcaataa
     2221 acatccataa cagcggcatg cggtcggact ggcgggaagg caccagcgtc aaaaactctt
     2281 ttagcgccat aaagctgacc agcgcgaaga acgtcagtgc cagccagcta ggacttaata
     2341 gtgcgaagga aaacaggacg ataatcaccc accaggtgcg aatacgtaat gtcagctcgc
     2401 gccagtcttt acttggacgc agccacacca gcaacccgtt gattacggtg gcaatcaaca
     2461 gcagggaaaa aataacgatc agtgattttt caagtagcgt catttcacac cctcttcacg
     2521 caatgcgctg cgacagcgat taaaaatcgt ccataacaac aacagtgttg cgacacccca
     2581 tatgacatta ttccaggcga caatctgcgg ccagatcgac agtgacagcc cccatgcacc
     2641 aaacaccagc gctctgtcgc ttttgcccag cggaccggca tagctgcgca cgccgttaat
     2701 cgtttgcgcc agcacgccgc aaaactcggt catcagcgca caaaacacca cgatcagcac
     2761 cagcggtata ctgctctgcg ggagtagcat aaagggcaga taaagcgaga tgtcggaaat
     2821 tacatcaccc gtctcattga ggatggcccc cagacgcgat ctttgattac attcacgcgc
     2881 cagcatacca tcgagcgcat tgagcgccat tcgtacaaac aacaccacgg gcagaaggac
     2941 gaataatccg ggctgaggat gaataatgag caccacgccg gtgataaggg aaagcaccat
     3001 cgcgcacagt gtaatgtgat tagcggtgac gccgcgcctg ttcagccaga acatcagcgg
     3061 tcttaacaga gcctgaaacg agggctttat ctggtaaagg gtcatcatca ctccttaata
     3121 agcatatttg acggggttga gtctggcctt ttattggggc tgtcactggc acactgaaag
     3181 tgaatacagt tccgggcgcg gaaatcctgc ctgctgcgcc ggtaaaggcg actgactggt
     3241 attacgggag gccagagagc cagtacctgt gccggtagac tgtgtggctg aaccataaac
     3301 atagtgtcct ggacaacggt acagcactct gtgcagctgt gtcagcatgc gcttttcttt
     3361 aattatgccc agtcatgtgc ggaggcaagg atttcaaaat atccgttaca tttgttgcat
     3421 gatgccttat cgctggtccg ggctattgct ttacttcttt taaatcagct ctgatgaaca
     3481 ttttttacca ctttatcgca ttaaggttat gtcattcatc gtctggcatc acgtttttgg
     3541 ttatggtgtc agggtgtacc ctaccgcgat aacgcaaccc taataaagtc attcgatggc
     3601 atatttctta ttgagaatat atacgcataa aatagttata aaaacgccta gtccctgaat
     3661 aaatgatata tcttcctgca ggaagaacca ggacaacagc aatgctacca gaggaataaa
     3721 atagagcatt gctcctgcta tgtgaacggg aatccagcgt agggaataaa gatagaaaca
     3781 aaaagccaaa aaatattgta aaattcctga caaaaacaca agaaatacag attctattgg
     3841 cagttgcagg gatgtgccag ttagaacaat aaaaaaacaa gcaagaataa aagagaaagt
     3901 caactgcccg gttaacaacg taacaggctc aaaattcaca ataagtcgtg atgagataac
     3961 aacataaaag gatgctgaga gtacacctgc aacaataagt atatatcctg taatattatc
     4021 aatgcggtca ttgatatcag gtagggcaac cattatactg cctgccattg ctccagtaag
     4081 caataatata aagtgta
//
LOCUS       DAZFKF010000222         4080 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFKF010000222 DAZFKF010000000
VERSION     DAZFKF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.224"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(18..710)
                     /locus_tag="ACHKA1_005264"
     CDS             complement(18..710)
                     /locus_tag="ACHKA1_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO5682781.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQLGKPTANHPKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
     gene            821..1048
                     /locus_tag="ACHKA1_005265"
     CDS             821..1048
                     /locus_tag="ACHKA1_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5682782.1"
                     /translation="MTNQTIQLAISITGSQKRLADLCGVAQPTVWRWLHGGGIDARYV
                     MKIVSATGGKIKPADIRPDLAPLFNASNSAA"
     gene            1079..1618
                     /locus_tag="ACHKA1_005266"
     CDS             1079..1618
                     /locus_tag="ACHKA1_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO5682783.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQAPGHEKIRDAVRAWSAADN
                     QDVVAALIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGHLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEMSEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            1615..2634
                     /locus_tag="ACHKA1_005267"
     CDS             1615..2634
                     /locus_tag="ACHKA1_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5682784.1"
                     /translation="MTGSEMAKAGLREQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     VAQKERRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGILIQSGRNIGINNVISEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSDESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            2631..3332
                     /locus_tag="ACHKA1_005268"
     CDS             2631..3332
                     /locus_tag="ACHKA1_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5682785.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGIATMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGARV"
     gene            3329..3631
                     /locus_tag="ACHKA1_005269"
     CDS             3329..3631
                     /locus_tag="ACHKA1_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO5682786.1"
                     /translation="MTGKEAIIHYLGTHKKFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            3699..4031
                     /gene="emrE"
                     /locus_tag="ACHKA1_005270"
     CDS             3699..4031
                     /gene="emrE"
                     /locus_tag="ACHKA1_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO5682787.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
BASE COUNT         1120 a          926 c         1031 g         1003 t
ORIGIN      
        1 ataattttta attggtacta tccatgcttc ctatacgtct gcggcatgct cccaataact
       61 ttcccgaaga taaatacccg gttcatctca tctttctcga tcgggtccca cggtgagtag
      121 cttttgttat cagagataac cagcagctta tccttcatca tttgcaggcg ctttacatgg
      181 gctgtgtcgt cgtacagaaa cgcatagata ccatcaccgt cgaaagattt aaccgtgata
      241 tcaacgaata gaagatcacc aggttcaatc gttcctgaca tgctgtcacc gcgtacgtta
      301 atgatgcgga tattttctgc cttcctacca tcgaacatgt gacgagcatc gtcaaacgag
      361 tactcaaccg agcgtagaac ttctacaaac tcacggttga tgactcccgg cccggcactc
      421 acttctatat caagaacgtc aatcttgaag tatttgggat ggttggcagt aggcttccct
      481 aattgttgac cgtcatttct catcgggcct atgcctgatg agagccactc tgttcgaaca
      541 cccaatgcat tagctatttc aacaattttt gttgagccgc gcgcgttgcc gcttgtcagt
      601 ctccagattg tgggttgagc tacgccagac gcctttgcaa gagcgccttg agacattcca
      661 gattgttcca tcgctaggtt taagcgatca gcaagagttt cttttttcat aagttttaat
      721 ttatacgctt gcgtattgat ggtcaaaaca cgttttgcta ttgttatgat taatacgtat
      781 tgctattatt tattcattgt aataccaata ggaattgata atgacaaatc aaaccattca
      841 actcgcaatc agtattacag gtagtcaaaa acgactggca gatctatgcg gtgtagccca
      901 gcccactgtt tggcgttggc tacacggtgg cggaattgat gcccgttatg taatgaaaat
      961 tgtctcagcc actggtggaa agattaaacc agcagatatt cgtcccgacc tcgcaccatt
     1021 gtttaacgcg agtaattctg ccgcctaatc tgcggcgtta actgataagg caatgactat
     1081 gcaaccactt acataccaac agactagcgg atttagcccg actgcggtga taaatcgttc
     1141 tcaaacaaaa caagctccag gccacgaaaa aatccgtgat gccgtccgcg cctggtcggc
     1201 tgcagataat caggatgttg ttgccgcact cattgtgaat gagtatcggg agcagggcgg
     1261 cggcaccatc gatttccctg atgatgtcag ccgtgcacgc cagaagctgt tccgcttcct
     1321 cgataacaaa ttcgattctg aaaaataccg aaataacgtg cgtgaactga ccccggcaat
     1381 tctggcggta ctaccgctgg aatatcgcgg ccacctggtt gagcaggata gcttcatggc
     1441 tcggctggct gaaatggaaa aggaactcag tgaggcaaaa caggctgtca ttctcaacgc
     1501 accacgccac cagaaactga aggagatgag tgaaggcatt gtgtcgatgt ttcgtgtgga
     1561 cccggatctg gctggtccac tgatggcgat ggtcaccacc atgctggggg caatatgaca
     1621 ggttcggaaa tggcgaaagc cggtctgcgc gaacagaacc gactttcagg tgcaaatcgt
     1681 aacacactca ttgcgggagg aattatggca aacactgctg agatattcaa ttttccagtg
     1741 ccggatgtgg cacaaaagga gcggcgcgtg gcagatctcg atgatggtta tacgcgcatt
     1801 gcaaatgagt tgctggaagc tgtgatgctg gccggattaa cacagcacca gcttctggtc
     1861 tttctggctg tcatgcgcaa aacatatggc tttaataaaa aactggattg ggttagcaac
     1921 gagcaacttt ccgaattaac cgggatattg ccgcacaagt gttctgctgc aaaaagtgtt
     1981 ctggtaaagc gtgggatttt gattcagagc gggcggaata taggcattaa taatgtgatc
     2041 agtgaatggt caacattacc cgaatcaggt aagaaaaata aagtttacct gaaagaggta
     2101 aatttacctg aatcaggtaa gaaaagttta cccaaatcag gtaaaggcgt ttacccgaat
     2161 caggtaaaca caaaagacaa actaacaaaa gacaatataa aacctttttc gtccgagaat
     2221 tctgacgaat cctctgacca gccagaaaac gatcttcctg tggtgaaacc ggatgctgca
     2281 attcagagcg gcagcaagtg ggggacagca gaagacctga ccgccgcaga gtggatgttt
     2341 gacatggtga agaccatcgc gccatcagcc agaaaaccga attttgcagg gtgggctaac
     2401 gatatccgcc tgatgcgtga acgtgatgga cgtaaccacc gcgacatgtg cgtgctgttc
     2461 cgctgggcat gccaggacaa cttctggtcc ggtaacgtgc taagtccggc caaactccgc
     2521 gacaagtgga cccagctcga aatcaaccgt aacaagcaac aggcaggcgt gacagccagc
     2581 aaaccaaaac tcgacctgac aaacacagac tggatttacg gggttgatct atgaaaaaca
     2641 tcgccgcaca gatgattaac tttgaccgtg agcagatgcg tcggatcgcc aacaacatgc
     2701 cggaacagta cgacgaaaag ccgcaggtac agcaggtagc gcagattatc aatggtgtgt
     2761 tcagccagtt actggcaact ttcccggcga gcctggctaa ccgggaccag aacgaactga
     2821 acgaaatccg ccgccagtgg gtgctggctt tccgggaaaa cgggatcgcc acaatggaac
     2881 aggttaacgc aggaatgcgc gtagcccgtc ggcagaatcg accattcctg ccatcacccg
     2941 ggcagtttgt cgcctggtgc cgggaagaag catccgttaa cgccgggctg ccaaacgtca
     3001 gcgagctggt tgatatggtt tacgagtatt gccggaagcg tggcctgtat ccggatgcag
     3061 agtcttatcc gtggaaatcg aacgcgcatt actggttggt taccaacttg taccagaaca
     3121 tgcgggccaa tgcgttgact gacgcggaat tacggcgcaa ggctgccgat gaactgtcct
     3181 gtatgaccgc acgaattaac cgtggtgagg ctatacctga accagtaaaa caacttcctg
     3241 tcatgggcgg tagacctcta aatcgtgcac aggctctggc gaagatcgca gaaatcaaag
     3301 ctaagtttgg gctgaaagga gcaagggtat gacgggcaaa gaggcaatta ttcattacct
     3361 ggggactcat aagaaattct gtgcgcagga cgttgccgcg gtaacaggcg caacggtaac
     3421 cagcataaat caggctgcgg ctaaaatggc gcgggcagga atcctggtcg ttgatggaaa
     3481 ggtctggcga acggtgtatt accggttcgc taccagagaa gaacgggaag gaaaggtgag
     3541 cacgaatctg atttttaagg agtgtcgcca gagtgccgcg atgaaacggg tattgagggt
     3601 atataaaaga acatcaatgg gtacacaatg atgaaacagg tgagttgagt tcaaactgta
     3661 gtacaattct ctccagtttg aacaggaaag aatatgctat gaacccttat atttatcttg
     3721 gtggtgcaat acttgcagag gtcattggta caaccttaat gaagttttca gaaggtttta
     3781 cacggttatg gccatctgtt ggtacaatta tttgttattg tgcatcattc tggttattag
     3841 ctcagacgct ggcttatatt cctacaggga ttgcttatgc tatctggtca ggagtcggta
     3901 ttgtcctgat tagcttactg tcatgggggt ttttcggcca acggctggac ctgccagcca
     3961 ttataggcat gatgttgatt tgtgccggtg tgttggttat taatttattg tcacgaagca
     4021 caccacatta aaagtaattt gtttctaaac gactaaaata tggaggctcg tatatttata
//
LOCUS       DAZFKF010000223         4009 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFKF010000223 DAZFKF010000000
VERSION     DAZFKF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.225"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            138..2231
                     /gene="cda"
                     /locus_tag="ACHKA1_005271"
     CDS             138..2231
                     /gene="cda"
                     /locus_tag="ACHKA1_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="HFO5682788.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAA
                     RTVLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPA
                     AGNNVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPV
                     SPNLPDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPEGS
                     GVSPLYISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGV
                     NQPPVYTQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFS
                     NALEKVTIPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGV
                     VIEPGATSVNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITL
                     PAVVGAPSRTILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDV
                     GGLRDFIYWRPDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNR
                     ETLTKFRDAIEEHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKIN
                     PDADNGRIYLETGEL"
     gene            2228..2491
                     /locus_tag="ACHKA1_005272"
     CDS             2228..2491
                     /locus_tag="ACHKA1_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="HFO5682789.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNI
                     LNCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
     gene            2561..2707
                     /locus_tag="ACHKA1_005273"
     CDS             2561..2707
                     /locus_tag="ACHKA1_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFO5682790.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIG
                     VAVQ"
     gene            3382..3600
                     /locus_tag="ACHKA1_005274"
     CDS             3382..3600
                     /locus_tag="ACHKA1_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682791.1"
                     /translation="MFFPALSPDSVDNTITAFEWADTARRSRTTERSESVSEEAEKRL
                     DEHFLLTHLCGISHPAWCTFHTILTDAA"
     gene            complement(3752..3943)
                     /locus_tag="ACHKA1_005275"
     CDS             complement(3752..3943)
                     /locus_tag="ACHKA1_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO5682792.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
BASE COUNT         1033 a          814 c         1077 g         1085 t
ORIGIN      
        1 ttttcacaaa aacgtaaatc cagtgggttg acaggattta cgaggtgacg taacttttta
       61 ctgtacataa aaccagtggt tttatgtaca gtttgttgtt tttaacgcat tgttttaaaa
      121 gtcaaagagg tgtttttatg agtgattacg aaggtagtgg tccgacagaa ggtattgatt
      181 atgggcactc gatggtcgtg tggccgtcaa caggactgat ttcgggtggt gatgtgaaac
      241 caggaggctc atcaggtatc gctccatcca tgcctccggg atggggggat tacagcccac
      301 agggtatcgc acttgtacaa agtgttcttt ttccgggaat tattcgccgg attattctgg
      361 ataaggaact tgaagaggga gactggtcgg gatggtctgt cagtgtgcat agcccttggg
      421 gaaacgagaa agtttccgct gcacgaacag ttcttgagaa tggtttacgt ggtggtttgc
      481 cagaaccgtc tcgcccggct gctgtttctt ttgcccgtct ggagcctgct tccggaaatg
      541 agcaaaaaat tattcgtctt atggttacac agcaactgga gcaggtaacg gatattccgg
      601 ccagccagtt accagcagcg ggtaataatg taccggtgaa atatcgtctg atggacctta
      661 tgcagaacgg tacgcaatat atggctatta tcggaggtat tccgatgaca gtgccggtag
      721 ttgatgccgt tccagttccg gaccggagtc gtccgggaac aaatattaaa gatgtttaca
      781 gtgcccctgt atcaccaaat ctaccggacc tggtattaag tgtgggtcag atgaatactc
      841 cggttctgtc taatcccgaa attcaggaag aaggagttat tgctgagaca ggtaattatg
      901 ttgaggctgg ttatacgatg tccagtaata atcatgatgt cattgttcgt tttcctgaag
      961 gcagtggtgt ttctccgtta tatatttcaa ctgtagagat tctggacagt aatggtctga
     1021 gtcagcgtca ggaagccgaa aataaagcaa aggatgattt cagagtcaag aaagaagaag
     1081 cggtagcccg ggctgaagct gaaaaagcaa aagccgagtt atttagtaaa gcaggtgtga
     1141 accagcctcc tgtatataca caggaaatga tggaaagggc taattcagta atgaatgagc
     1201 agggagctct tgttctgaat aacactgcca gttctgtaca actggcgatg accggaactg
     1261 gtgtctggac tgctgctggt gatatagcag gcaacataag caagtttttc agtaatgctc
     1321 tggagaaagt aaccataccc gaagtgagtc ccctgcttat gcggatttct cttggtgctc
     1381 tgtggtttca ttcagaagaa gctggagcag gaagtgatat cgtgccgggg cgaaatctgg
     1441 aggcgatgtt ttcgctgagt gctcagatgt tggctggaca gggcgtggtc attgaacctg
     1501 gtgcgacgag tgtaaatctg cctgttcgtg gacaattgat aaacagtaac gggcaattag
     1561 ctctggattt actgaaaaca gggaatgaaa gtatcccagc tgcggttcct gttcttaatg
     1621 ctgtccgtga tacagcaaca ggactggata aaatcacgtt accagcagta gtaggcgcac
     1681 cttcccggac gattctggtt aatccggtac cacaaccttc ggtgccaaca gatacaggta
     1741 atcatcaacc cgttccggtt acaccagtgc atacaggaac ggaagtaaaa ccggtcgaaa
     1801 tgccagtaac gacgattact cctgtttctg atgttggtgg actacgagat tttatttact
     1861 ggcgtcctga tgctgctggg actggtgttg aagctgttta tgtgatgctt aatgatcctc
     1921 tggattcagg gcgattttcg cgtaaacaac ttgataaaaa atataaacat gctggtgatt
     1981 ttggtattag tgatacaaaa aagaatcgtg aaactcttac taaattcagg gatgctattg
     2041 aggagcattt atcggataag gatacagtag agaaaggaac ataccgaaga gaaaaaggtt
     2101 caaaagttta ttttaatcct aatacgatga atgtggttat aattaagtca aatggtgagt
     2161 tcttatctgg gtggaaaata aatccagatg cggataatgg tcgaatttat ttagagacag
     2221 gtgaactatg aacaagatgg caatgatcga tttggcgaaa ttatttttag cttcgaaaat
     2281 tacagcaatt gagttttcag agcgaatttg tgttgaacgg agaagattgt atggtgttaa
     2341 ggatttgtct ccgaatatat taaattgtgg ggaagagttg tttatggctg ctgagcgatt
     2401 tgagcctgat gcagataggg ctgattatga aattgatgat aatggactta aggttgaggt
     2461 ccgatctatc ttggaaaaat ttaaattata atcatgagtg attttaatat ctggcattta
     2521 agtataaatg tcagatattc actcatgaca aggagatgtc atgaaaaaaa agataggggg
     2581 atctatgatt ctcgttcttg ctgttttgtg tctgactgct tgccaagcta actatgtaag
     2641 ggatgttcag ggtggaacaa ttgctccatc atcatcttcg aaactaatcg gggtggcggt
     2701 tcagtgaaaa atattaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2761 ctgtaaacga aaaaacccgc cttgtggcgg gtccttccgt ccgatcagaa gtcttcgacc
     2821 tcttctaatc ggcggtggta actggcttgg aggagcacag tcaccaaaac tgtcttttca
     2881 gtttagcctt aactggttca taacttcaag agcaactcct ctaaatcagt taccaatggc
     2941 tgctgccagt ggcgttaagt cgtgtctttt ccgggttggc cccaagagaa cagttaccgg
     3001 agaaggtgca gcagtcgggc tgaacggggg gttcctgcat acagcccagc ccggagcgaa
     3061 ctgcctaagt cgccccgaga taccagaaga cgtgagctat gagaaagcgc cacgtgtccc
     3121 gcaagggaga aaggcggaaa aggtatccgg taatcgatat ggcgggaacg tgggggcgca
     3181 ggagggagcg attcaccgga aacggtgatg atctttaagc cacgtcgggt ttcgccaccg
     3241 ctggcggatt tcatggttga gcctcatggc tcccacagat gcacagaaaa agcgtctgtt
     3301 ttgtgaagtc cgtcaggggg gcggagccta tggaaaaacg ccagcggcgc gacttttttt
     3361 gccctttgcc tttttgctca catgttcttt ccggccttat cccctgattc tgtggataac
     3421 actatcaccg cttttgagtg ggcagatacc gctcgccgca gccgaacgac cgagcgtagc
     3481 gagtcagtga gcgaggaagc ggaaaagcgc ctggacgagc attttctcct tacgcatctg
     3541 tgcggcattt cacacccggc atggtgcact tttcatacca ttctcactga tgccgcataa
     3601 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     3661 cgctaaagcc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gctcacaggc
     3721 cagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tccccgaagc
     3781 gtgcgaggca gctgcggtaa agctcgtcag cgtggtcgtg aagcgcttcg cagatgtcag
     3841 cctggtcgtc ggcgtccagt tcattcagtt tttccagcag ggtcagcgtc tggcttctga
     3901 tgaagcgggc catgttaagg gcggtttttt cctgtttagt cacagttctg tttctccatg
     3961 tgtggggagt ctgtcggagg tttttaatga taccggtgaa acgcgaaag
//
LOCUS       DAZFKF010000224         3975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFKF010000224 DAZFKF010000000
VERSION     DAZFKF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.226"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..560
                     /locus_tag="ACHKA1_005276"
     CDS             <1..560
                     /locus_tag="ACHKA1_005276"
                     /inference="COORDINATES: protein motif:HMM:NF018697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5682793.1"
                     /translation="NMRQEQDFSFVEEGKESIQDRIRQLIGSRSMRQAAIDWGLSYST
                     LNNYFEKGTTPGLKVVSNICKVENVSLEWLILGTKTIQPQESTRPTIASSDERDYQTI
                     SAIWSSLEPTELDELSRKLGRKGADFLTILLDNDIQELHSLHGQRRALALSLNDLSDE
                     QIREICEFCKTKVDHFNVTYKKTSA"
     gene            complement(834..2933)
                     /locus_tag="ACHKA1_005277"
                     /pseudo
     CDS             complement(834..2933)
                     /locus_tag="ACHKA1_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
     gene            complement(2953..3414)
                     /locus_tag="ACHKA1_005278"
     CDS             complement(2953..3414)
                     /locus_tag="ACHKA1_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO5682794.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(3442..>3975)
                     /locus_tag="ACHKA1_005279"
     CDS             complement(3442..>3975)
                     /locus_tag="ACHKA1_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682795.1"
                     /translation="PGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGKEQVYIHAQKD
                     MDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRLLMVEKGDKITGVRKGSLSENVFQ
                     DRGTIAGSVHVDAVDNGGEGNGIQAYTAIKEIMLAVEESKIALTPDGIQLQVGESTVI
                     RLSKDGITIVGGSVFIN"
BASE COUNT          859 a         1093 c         1009 g         1014 t
ORIGIN      
        1 aaaacatgag acaggaacaa gatttttctt ttgtcgaaga gggaaaagaa agtatccagg
       61 acaggatcag gcagctaatc ggcagcagaa gtatgcggca agcagctatc gattgggggt
      121 tgtcatactc aacattgaat aactactttg agaaagggac cacacctggc ctaaaagtag
      181 ttagcaatat ttgcaaggta gaaaacgtat cgttggaatg gctcatattg ggcacgaaaa
      241 caatacagcc acaagaaagt actcggccga caattgcgtc ttcagatgaa cgagattacc
      301 agacgattag cgctatatgg agctctctag aaccaacaga acttgatgaa ttatctagga
      361 aattaggccg caaaggcgca gattttttaa ccatactttt agataatgat attcaggaat
      421 tacatagctt acacggtcaa cgtagagcac tagccttaag cttaaatgat ttatctgatg
      481 aacagattag agagatttgc gagttttgta agactaaggt tgatcacttc aatgtaacct
      541 ataaaaagac cagcgcgtaa gggagtggtg caaattttgt gctgtggtac actcctttaa
      601 agacaatctg gagaaaagcg ttttaagcct ttttaaagcc attttaagcg tcaatccgta
      661 gtatcaaaag tgttgccgac tttagaataa acgactaatt attgcacaat cggtatcaaa
      721 tagactttcc gcgctcgcat tagcatcacc tgttttattt cagtatctta caggtgattt
      781 cacctccttt cactaaaacc ctcgttggta tcaaatgatt cacctggtta cacccattca
      841 tacgtggtgg tgctgccgtc cggcgcttct tcccgcagaa tattgccgtc aggatcatag
      901 taataacggt gagcgccctt gccgtccgtc acggtggtgc agccgtcccc atagtccagc
      961 gtggcgttgt aatagccctc cgccccggtg acggtcacgc accggccctg ttcatcgtag
     1021 gtgaaacggc tccacgtctg gtcgttatcg gaccagcgga tgatccggtc cgcagcgtcg
     1081 tactcataaa acaggtggta gtccagccgc gcatccgctt ctgtcagccg cccccgcgca
     1141 tcctgttcgt aggtcgccag cgtctgtatg ccgttatccg tgcggtgaat ggctttcagg
     1201 tagccgctgg cgtggtacgt cagtttaagc cggatcccgt cggtgtgact gacacccgtg
     1261 atccggtact ggctgtcgcg ctcaaacgtg atgcggttgc tggcggtgtc gctcatcccg
     1321 cccagcatgt acatccggtc tgcggggctg tgtgccaggc ggtcatcgct ggtatcgtca
     1381 gtcaccgggt ggcgcagacg cattcccgga aaggcgtcat aaaagaagcg gctgctcagt
     1441 gcatccctgt cgaacagctc cagataatca tcacggcggc gcagaatgta ctgggcgtga
     1501 tacgggttaa ccgccgtatc actttcaccg tcgaaggcga agttcagcgt ggctcccagg
     1561 ctgatgatat ccacccggtt tccctgttca cgcacccagg cgtattcgct ccaggagtca
     1621 ctccagccca cgcccgtcag gccatgcagt ggcagtaccg aactgtggaa acgggtgaag
     1681 gagaggggga gggtctggcc cagggtgaag tcggtacgtt cgtcgcagta cgcgccggtt
     1741 accggatcga caggatctgt tacccatctg atgactttat tggcaatact ttttaacgac
     1801 ttacctgttt tccccagcaa cgcgccgagt tttgcgccca gcagctttcc gcttttcccc
     1861 agcaggtttc ccagtttgcc cagtttcgcc agattcccca gttttcccag cagtcctgct
     1921 ggcggcagaa gatagctggc tatcgttatt cctcccagga tcattctctg ccatggcggg
     1981 aactccggct ggatatccag ataggtgacc tgctcacccc caataaagac gtccggcgaa
     2041 ccttccacga tcgccgcact gcactccagt ttgtccccct ggcgcgcggc aggctgattg
     2101 ttgataaaga cgttgctgct gccctgggct atcctcggtg gtttgttctc cttgtcgcag
     2161 agcgccgtgt cctcaccggc ttccgggaaa tccacatctt ttttgatttc gccacgcccg
     2221 cccaccatcg tggcaaattc cattaatacc gggccgctgg gaaacagaag cgatgcgcct
     2281 gcactgacgc gatccataaa actggcattg ctgtccgtca gggtgataac agcgttcccc
     2341 caggccttcg ccagtggtac tgctttttcc gctgccgatt caagcaggtc agtggcaata
     2401 tcaccccatg agggttcccc ctgtgggact tcaggtattg ctccaggtgg gggaggaggg
     2461 ggaagggtga cggctgcacg ggctgctggc tttccgttaa tttttacgtt ttcagaaccg
     2521 gtattgatgg cgccgtccgt gtcacaaaag ctgtttatcc atgattcaag ctggttctgt
     2581 ttctcctcca cataatcact cgccttatag ctggcataca tcagcgcagg caccagaacc
     2641 agcgcataag ggccggtggc acaggcggcc ataatcaggc aaccttctgc gatggtcact
     2701 atcgccccca gcacggcccc tgccagcgcc cccaaaaaac tcttgtgctg aatttcatcg
     2761 cccagcctgg cggcggggga gccagacttt tttgatgcca acatctgtgc aatggctttg
     2821 tcgttggctg cccgtttcat ctggctggcg accatcgcct gctgtgacag cattcgcatt
     2881 gccagcgtac cgcctgcggt cgctccctgt ggcccgcctg gtccttcact catgtgttat
     2941 ccctcgttta tgtcagcgcg gctggtcctt ttggatgtca ttctccggtg gcaacggggt
     3001 gaagctcttt atcacctcca gcagcatcgt cttttgactg ttcttcaggg ataaggaggt
     3061 gctgaaagtg aaggtcagca gcgtatctcc acgccgctgg aatacggcta tctggctaat
     3121 ccgcccctga ttcccttcat cttccggacc gttccaggaa taatccagca gccaggccgg
     3181 ggcctctgcc agagtgattt cccggcaggc atgttcggtg tacccatcaa gtttgccctg
     3241 gtacagcgcc agcgtttcct ggtacagctc tggtgtcagg gttccctgtg gaacggcttc
     3301 gcgggtaatg accaggttga tgccactgtt atctgtggac accaggacct gcattgactg
     3361 atcctgccat tgagacggca gttgaatgtg gccttcatta aaagtgaact gcataaaata
     3421 ctccctgata atctgagtga atcagttgat aaaaacagaa ccgcccacga tggtgatgcc
     3481 atccttcgac agcctgatta ccgttgattc cccgacctgt agctgaatgc catccggcgt
     3541 cagcgcaatt ttgctttcct ccacggccag cataatttcc ttaattgccg tataagcctg
     3601 tataccgttg ccttcgccac cattgtctac agcgtcaaca tgcaccgaac cggcaatcgt
     3661 gcctctgtcc tgaaagacat tctcagataa agaacctttt cgtactcctg tgatcttgtc
     3721 gcctttttcc accataagaa ggcgattacc ttcctgtatc gtgaccgtct ggtcattttt
     3781 tacggtttcg cgatgatcgt gttttacggt ggtggtacga tcgttgagta cctcggtatc
     3841 catgtccttc tgcgcgtgga tgtagacctg ttctttccct gtcgcatcgt caaacttcag
     3901 ctcattaaac ccgctgccca tatacgtttt ggaacggatg gtcatctgcg tcttcgtccc
     3961 cggcaggctg ccggg
//
LOCUS       DAZFKF010000225         3936 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFKF010000225 DAZFKF010000000
VERSION     DAZFKF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.227"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..3219
                     /locus_tag="ACHKA1_005280"
     CDS             <1..3219
                     /locus_tag="ACHKA1_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5682796.1"
                     /translation="ISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITRTI
                     TSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTTSQ
                     YLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALFGV
                     QVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCLWD
                     MLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRKAW
                     DVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSALKD
                     RHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLWLI
                     KTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLDRE
                     ITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTLRQ
                     RLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTA
                     EVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQLA
                     LGNYRLTVRAANAWGQQGDPASASFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTV
                     QFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAF
                     VEAVGRASDDAEGYLDFFKGQITESHLGKELLEKVDLTEDNASKLEQFSKEWKDANDK
                     WNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMF
                     VAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGTLN
                     NVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQVMI
                     PPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMPAG
                     QGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            3286..3885
                     /locus_tag="ACHKA1_005281"
     CDS             3286..3885
                     /locus_tag="ACHKA1_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5682797.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDVAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT          929 a         1002 c         1200 g          805 t
ORIGIN      
        1 atatccggcg tcacggtggt gttccgggcc ggtgagcagg agcagacacc gccggaggga
       61 tttgaatcct ccggctccga gacggtgctc ggtacagaag tgaaatatga cacgccgatc
      121 acccggacca tcacgtcggc aaacattgac cgtctgcgtt ttactttcgg cgtgcaggca
      181 ctggtggaaa ccacctcaaa gggggacagg aatccatcgg aagtccgcct gctggttcag
      241 atacaacgta acggtggctg ggtgacggaa aaagacatca ccattaaggg taaaaccact
      301 tcacagtatc tggcctcggt ggtggtggat aacctgccgc cgcgcccgtt cagtatccgg
      361 atgcgcagga tgacgccgga cagcaccaca gaccagctgc agaacaaaac gctctggtcg
      421 tcatacaccg aaattatcga tgtgaaacag tgctacccga acacggcact gttcggcgtg
      481 caggtggact cggagcagtt cggcagccag caggtgagcc gtaattatca tctgcgcggg
      541 cgtattctgc aggtgccgtc gaattataac ccgcagacgc ggcaatacag cggtatctgg
      601 gacggaacgt ttaaaccggc atacagcaac aacatggcct ggtgtctgtg ggatatgctg
      661 acccatccgc gctacggcat ggggaaacgt cttggtgcag cggatgtgga taaatgggcg
      721 ctgtatgtca tcggccagta ctgcgaccag tcggtgccgg acggctttgg cggcacggag
      781 ccgcgcatca cctgtaatgc gtacctgacc acacagcgca aggcgtggga tgtgctcagt
      841 gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct gacgttcgtg
      901 caggaccgac catcagataa ggtgtggacc tataaccgca gtaatgtggt gatgccggat
      961 gatggcgcgc cgttccgcta cagcttcagc gccctgaagg accgccataa tgccgttgag
     1021 gtgaactgga ttgacccgaa caacggctgg gagacggcga cagagcttgt tgaagatacg
     1081 caggccattg cccgttacgg tcgtaacgtc acgaagatgg atgcctttgg ctgtaccagc
     1141 cgggggcagg cgcaccgcgc cgggctgtgg ctgattaaaa cggaactgct ggaaacgcag
     1201 accgtggact tcagcgtggg tgcggaaggg cttcgccatg taccggggga tgtcattgaa
     1261 atctgcgatg atgactatgc gggtatcagc atcggcgggc gcgtgctggc ggtgaacagc
     1321 cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg taccacgctg
     1381 ataagcctgg ttgacggaag tggcaatccg gtcagcgtgg aggttcagtc cgtcaccgac
     1441 ggcgtgaagg tgaaagtgag ccgtgttcct gacggcgttg ccggatacag cgtgtggggg
     1501 ctgaagctgc cgacgttgcg ccagcgcctg ttccgctgtg tgagtatccg tgagaacgat
     1561 gacggcacgt atgccatcac tgccgtgcag catgtaccgg agaaagaagc catcgtggat
     1621 aacggggcgc actttgacgg cgaccagagc ggaacggtaa acggtgtcac gccgccagca
     1681 gtgcagcacc tgaccgcaga agtcaccgca gacagcggtg aataccaggt gctggcgcgc
     1741 tgggacacgc cgaaggtggt gaagggtgtg agctttatgc ttcgcctgac cgtggcagcg
     1801 gatgacggca gtgagcggct ggtcagcacg gcccggacga cggaaaccac ataccgcttc
     1861 acgcagctgg cgctgggaaa ctacaggctg acagtccggg cggcaaatgc ctggggacag
     1921 cagggcgatc ctgcatcggc atcgttccgg atcgccgcac cggcagcgcc gtcgcggatt
     1981 gagctgacgc caggctattt tcagataacc gccacgccgc atcttgccgt ttatgatccg
     2041 acggtacagt ttgagttctg gttttcggaa aagcggattg cggatatcag gcaggttgaa
     2101 accacagccc gctatcttgg tacggcgctg tactggatag ccgccagtat caatatcaaa
     2161 ccgggccatg attattactt ttatatccgc agtgtgaaca ccgttggcaa atcggcattt
     2221 gtggaggccg tcggtcgggc gagcgatgat gcggaaggtt acctggattt tttcaaaggc
     2281 cagataaccg aatcccatct cggcaaggag ctgctggaaa aagtcgacct gacggaggat
     2341 aacgccagca aactggagca gttttcgaaa gagtggaagg atgctaacga taagtggaat
     2401 gccatgtggg gcgtcaaaat tgagcagacc aaagacggca aacattatgt cgcgggtatt
     2461 ggcctcagca tggaggacac ggaggaaggc aaactgagcc agtttctggt tgccgccaat
     2521 cgtatcgcgt ttattgaccc ggcaaacggg aatgaaacgc cgatgtttgt ggcgcagggc
     2581 aaccagatat tcatgaacga tgtgttcctg aagcgcctga cggcccccac cattaccagc
     2641 ggtggaaatc caccggcatt ttccctgaca ccggacggaa agctgaccgc taaaaatgcg
     2701 gatatcagtg gcagtgtgaa tgcgaactcc gggacgctca acaacgtcac gattaatgag
     2761 aactgtcaga ttaaggggaa actgtcagcc aaccagattg aaggcgatat tgtcaaaacg
     2821 gtcagcaagt ctttcccccg cacgagcact tatgccagcg gcaccatcac ggtaagaatc
     2881 agtgatgatc agaaatttga ccggcaggtc atgataccgc cagtgttatt ccgcggtggt
     2941 aagcatgaga atttcaacag taataaccaa cagtcatact ggtattcaac ctgccggtta
     3001 agagtgaccc gcaatggtca ggagattttt aatcagtcca cgacggatgc tcagggcgta
     3061 ttttcctcag ttatagatat gcctgccgga caggggacgc tgacactgac attcaccgta
     3121 tcttcatcag gagcgaataa ctggacacca acaaccagta tcagcgatct gctggttgtg
     3181 gtgatgaaaa aatccacagc aggtatcagt atcagctgaa ttttataacc cagaacgggc
     3241 gtcagaaatg acgccttttt tattgcagaa aagcgagagg taattatgcg taaactttat
     3301 gccgccattt tgtccgcagc catttgtctg gccgtatccg gtgcgcctgc atgggcatct
     3361 gaacatcagt ccacgctgag cgcggggtat cttcatgccc ggacgaacgt tcccggcagt
     3421 gatgatctga acgggattaa cgtgaaatac cgttatgagt ttacggacac gctgggaatg
     3481 gtgacgtcat tcagctatgc aggagacaag aatcgccagc ttacccgtta cagcgatacc
     3541 cgctggcatg aagattccgt gcgtaaccgc tggttcagcg tgatggcggg gccgtctgta
     3601 cgcgtgaatg aatggttcag cgcgtatgcg atggcgggtg tggcttacag ccgtgtgtcg
     3661 actttctccg gggattatct ccgcgtaact gacaacaagg ggaaaacgca cgatgtgctg
     3721 accggaagtg atgacggtcg ccacagcaac acgtctctgg cgtggggggc tggcgtgcag
     3781 tttaacccga ccgaatccgt ggccattgat gtcgcttatg aaggttccgg cagtggtgac
     3841 tggcgcactg acggtttcat cgtgggtgtc ggttataaat tctgattagc caggtaacac
     3901 agcgttatga cagcccgccg gttcaggcgg gctttt
//
LOCUS       DAZFKF010000226         3897 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFKF010000226 DAZFKF010000000
VERSION     DAZFKF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.228"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..123
                     /locus_tag="ACHKA1_005282"
     CDS             <1..123
                     /locus_tag="ACHKA1_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5682798.1"
                     /translation="LSGGYRAVRDCWRRQIDKRAAEKISASQSAGSKPEEPITP"
     gene            149..394
                     /locus_tag="ACHKA1_005283"
     CDS             149..394
                     /locus_tag="ACHKA1_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5682799.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLEARLDAEVDA
                     ILDELLGAPAVPEPEGIAGEGSASDGGDPTPDSDMMM"
     gene            471..686
                     /locus_tag="ACHKA1_005284"
     CDS             471..686
                     /locus_tag="ACHKA1_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5682800.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            690..917
                     /locus_tag="ACHKA1_005285"
                     /pseudo
     CDS             690..917
                     /locus_tag="ACHKA1_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
     gene            1570..1857
                     /locus_tag="ACHKA1_005286"
     CDS             1570..1857
                     /locus_tag="ACHKA1_005286"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682801.1"
                     /translation="MQPNTGAHSSTFGVPENMQSVLSNADELLLIHLTKEKRSESLVC
                     LNIQQNPQGVSRSVPGVYESHNIDRDDIYEWLFYPMVVLCVPVNGGLPLDR"
     gene            1992..2525
                     /locus_tag="ACHKA1_005287"
     CDS             1992..2525
                     /locus_tag="ACHKA1_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5682802.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKKKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     ISRRDQESALACWGIDR"
     gene            2799..3494
                     /locus_tag="ACHKA1_005288"
     CDS             2799..3494
                     /locus_tag="ACHKA1_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO5682803.1"
                     /translation="MKEMISVDREISMSSLDFLNNIINPARAEAGEVPHEPRKFLAKI
                     EDELELDGTGKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     IPQTLPEALRFAAKLAEQKAELENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFRLFLLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKFTPKGVQW
                     IAGLLAAWRATAA"
     gene            3589..3720
                     /locus_tag="ACHKA1_005289"
     CDS             3589..3720
                     /locus_tag="ACHKA1_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682804.1"
                     /translation="MSDKLIMLAKGLCVIVGISFSLMLVALFLSITWMALTSAGLVG"
     gene            3732..>3897
                     /locus_tag="ACHKA1_005290"
     CDS             3732..>3897
                     /locus_tag="ACHKA1_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5682805.1"
                     /translation="MLSAFTVILLVVCGALSLGLNHYRDSAITYKAQRDKKARELELA
                     NATITDMQVRQ"
BASE COUNT         1048 a          830 c         1068 g          951 t
ORIGIN      
        1 ctttcgggtg ggtacagggc agtgcgcgat tgctggcgca ggcagattga taaaagggcc
       61 gcagagaaaa tcagcgccag tcagtcagcc ggaagcaaac ccgaagagcc cattactcct
      121 taataacccc tttcaacgag aaaatcccat gtcagaaatt acatccctgg tcactgcaga
      181 ggcagtgaag gaagtcctgc gctctgaaga agtcctgagc gcactgaaac agaaactccg
      241 ccagaacctt gaggcgcgtc ttgatgcaga agtggatgcc attctggatg aactgctggg
      301 cgcaccggca gttccggagc cggaaggtat cgcgggtgag gggagtgctt cagatggcgg
      361 tgaccccaca cctgacagcg acatgatgat gtaagcatgc gcaagggacc atcggtgtgt
      421 gccggtggtc tttatattgt tgtgagcttc cggattgcgg gagacggggt atgtaccaga
      481 tggaaaaaat cacaacaggt gtgtcataca ccacgtcagc ggtgggaacg ggctactggt
      541 tcctgcagtt gctggacagg gtttccccgt ctcagtgggc ggcaataggt gtgctgggga
      601 gtctgctgtt tggtctgctg acatatctga cgaacctgta tttcaaaatc agagaggacc
      661 gtcgtaaggc ggcgcgggga gagtagacga tgaataaaaa atatgaactg gttgtaaaag
      721 ggataaatca ttactcggat aagataactg ttactgtgac actggaaatt gccgggcagc
      781 cgtcgttgtt gtcgccatat gtggcgatta gtcttgaccg tactgaaggt gccacgctgg
      841 aattttacga agctgaggcg aaaaaacagg cgaagcagtt tttcatggat attgctgccg
      901 ctttatgtga aggggatgaa cagtcacagg aaaagcgcct ctgttcagaa gagcggtata
      961 ccatacaaat taacaatgca tataacacga tacagtcaga aaaagatgat atcgaatcac
     1021 gcattgaaaa actggagaac agcgtcgttg aactgaataa aaaactgtcg gtgcagatcc
     1081 cttccgaagg tgaaaaaaaa cgccgtgatg agcagttcgc ggcgttttac gattattgcc
     1141 ggaaagtgat gagcagaaat ctcgcagagt gtttccgtat tcaaaaaaac agagagcgct
     1201 ctgcaaatag tgagattctg gataattttt taaaggggac gcatctcgcc agtgattgtt
     1261 tcattgctca ttcttttggt ggctttcatg aggccatcaa acatgatgtt gagagaagtc
     1321 caggtatcct ctgttccatt cccgtaagca tcgaaataga cacaaatacg ataaccggtg
     1381 aactcgttac cgccggagtc ggtatattcc gtcatgaggg gaacgatgcc aaaggttcct
     1441 ttacaaaaat cgggccgttc tttctgaagg tagagcgata gtttgttaag tacccatccg
     1501 acagctctct cattttggtt tattttcgta tcgcgaaaca aaaattcgaa gcggccagat
     1561 gacgcatggg tgcaaccaaa taccggggcg cattcatcaa cctttggtgt tcctgaaaac
     1621 atgcagtccg tgctctcgaa tgcagatgaa ttgctgttga tccacttaac aaaagaaaaa
     1681 aggtctgaat cgcttgtctg tctgaatatt caacaaaatc ctcaaggtgt gtctcgatca
     1741 gttccgggtg tttacgaaag tcataatatt gaccgggacg atatttatga gtggttgttt
     1801 tatcccatgg ttgtgctctg cgtccctgta aacggaggtc tgccgctgga tagataagca
     1861 taagtaatct cctggtggaa tgtaatccac gatcatccgg ggaaattaag aacccgccag
     1921 tgcccaccac tggtggggtg aaggcttaac atatccgggt attcggaacc gataaatcct
     1981 gataaatatc catgaacgca aaaatcagat acggcctgtc ggctgccgtt ctggcgctga
     2041 ttgccgcagg ggcgcctgcg cctgatattc tcgaccagtt tctggatgaa aaagaaggta
     2101 accacaccac ggcataccgt gatggtgcgg gtatctggac catttgtcgt ggtgccacca
     2161 gggtggatgg caaacctgtc gtcccgggca tgaagttgtc gaagaaaaaa tgcgaccagg
     2221 ttaacgccat tgagcgtgat aaggcgctgg catgggtgga gaaaaacatc cgggtgccac
     2281 tgaccgaacc ccagaaagcg gggatcgcgt cattctgccc gtataacatt ggtcccggta
     2341 agtgtttccc gtcgacgttt tataaacgaa ttaatgcagg cgatcgcagg ggagcgtgtg
     2401 aggcgattcg ctggtggatt aaggacggtg gcagagactg ccgtattcgc tcaaataatt
     2461 gctacggtca gatatcccgt cgtgaccagg agagtgcgct ggcgtgctgg ggaatcgaca
     2521 gataagcaga atattttgct gaaaaatgac gttggtcaac gcgggtggat aacacgaaat
     2581 cctgcgaact ggcaaaacct aagtgaataa agtcaggaac attgtttcac gcagaggcac
     2641 cgtaatggtg cctttgtcat ttctgcgctt cgcacaagcg taaataaacc aaagaacctt
     2701 tcaggatgag ccctggtgga taaccggcag tggtctggtt aaccctcttt gggctggtta
     2761 ttcctgtgcg cagggttcat cactaaaagg aatcaaccat gaaagagatg atttctgtcg
     2821 atcgtgaaat atccatgagt agtctggatt ttctgaataa cattattaat ccagcccggg
     2881 cagaagccgg agaagtccct catgaaccgc gtaagtttct tgcaaaaatt gaggatgagc
     2941 tagagcttga tggaaccgga aaaaaattcc ggttaaacaa taaccagaca agaacggcat
     3001 actatgatct ggattttgac cagatgatgc tcgttggcat gagagagtca aaggccgttc
     3061 gtcgttctgt gctggcaagg ctcaaagcga tgcatggtat tcagatcccc cagactttac
     3121 ctgaggcgtt gcgatttgcg gcaaaactgg cagaacagaa agcagagctg gaaaatcaac
     3181 tggcaatagc agcaccgaag gctgaatttg ttgataacta tgttgaagca tctggtctga
     3241 tgggattccg ggaagttgct aagttactcg gtatcaaaga aaccgatttc cggctgtttt
     3301 tgttggagaa cggaataatg tatcgcctcg ctggaaaaat gacgccttac tcgcatcacc
     3361 tggatgcggg gcggtttagc gtgaaaacgg gcgaggcggg caatggtcat gcttttacgc
     3421 aggttaaatt caccccaaaa ggtgttcagt ggattgctgg tctgcttgct gcatggagag
     3481 ctaccgcagc atgaagatga taaaaatgga ctggaaattt ttgctggtct ggctgattcc
     3541 gtttttatgg gtggttgccc ggttaattac tgctattaag aggtaaagat gtcagacaaa
     3601 ctcataatgc tggcgaaggg cctttgtgta atcgtcggta tttcattttc actaatgctg
     3661 gttgctcttt ttctttccat aacctggatg gcgttgactt cggcagggct ggtggggtga
     3721 gcataaaccg aatgctttcc gcgtttaccg ttattctgct ggtggtctgt ggtgcgctta
     3781 gtctggggct gaatcattac cgcgatagcg ccatcaccta caaagcgcag cgcgataaaa
     3841 aagccagaga gctggagctg gcaaacgcaa ccattactga tatgcaggtg cgccagc
//
LOCUS       DAZFKF010000227         3876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFKF010000227 DAZFKF010000000
VERSION     DAZFKF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.229"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            49..1434
                     /gene="dcuC"
                     /locus_tag="ACHKA1_005291"
     CDS             49..1434
                     /gene="dcuC"
                     /locus_tag="ACHKA1_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO5682806.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1474..2154)
                     /gene="dpiA"
                     /locus_tag="ACHKA1_005292"
                     /gene_synonym="citB"
     CDS             complement(1474..2154)
                     /gene="dpiA"
                     /locus_tag="ACHKA1_005292"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO5682807.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2123..3781)
                     /gene="dpiB"
                     /locus_tag="ACHKA1_005293"
     CDS             complement(2123..3781)
                     /gene="dpiB"
                     /locus_tag="ACHKA1_005293"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO5682808.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGHPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          895 a         1002 c          935 g         1044 t
ORIGIN      
        1 ttaggaattt tttattattt actttggggc ctggagacag gaaaaattat gctgacattc
       61 attgaactcc ttattggggt tgtggttatt gtgggtgtag ctcgctacat cattaaaggg
      121 tattccgcca ctggcgtgtt atttgtcggt ggcctgttat tgctgattat tagtgccatt
      181 atggggcaca aagtgttacc gtccagccag gcttcaacag gctacagcgc cacggatatc
      241 gttgaatacg ttaaaatatt gctaatgagc cgcggcggcg acctcggcat gatgattatg
      301 atgctgtgtg gctttgccgc ttacatgacc catatcggcg cgaatgatat ggtggtcaag
      361 ctggcgtcaa aaccattaca gtatattaac tccccttacc tgctgatgat tgccgcctat
      421 tttgtcgcct gtctgatgtc tctggccgtc tcttccgcaa ccggtctggg tgttttgctg
      481 atggcaaccc tgtttccggt gatggtaaac gttggtatca gtcgtggagc agctgctgcc
      541 atttgtgcct ccccggcggc gattattctc gcaccgactt caggggatgt ggtgctggcg
      601 gcgcaggctt ccgaaatgtc gctgattgac ttcgccttca aaacgacgct gcctatctca
      661 attgctgcaa ttatcggcat ggcgatcgcc cacttcttct ggcaacgtta tctggataaa
      721 aaagagcaca tatctcatga aatgttagat gtcagtgaaa taaccaccac tgcccctgcg
      781 ttttatgcca ttttgccgtt cacgccgatc atcggggtgc tgatttttga cggcaaatgg
      841 ggtccgcaat tacacatcat cactattctg gtgatttgta tgctgattgc ctccattctg
      901 gagttcctcc gcagctttaa tacccagaaa gttttctctg gtctggaagt ggcttatcgc
      961 gggatggcag atgcatttgc aaacgtggtg atgctgctgg ttgccgctgg ggtattcgct
     1021 caggggctta gcaccatcgg ctttattcaa agtctgattt ctatcgctac ctcgtttggt
     1081 tcggcgagta tcatcctgat gctggtattg gtgattctga caatgctggc ggcagtcacg
     1141 accggttcag gcaatgcgcc gttttatgcg tttgttgaga tgatcccgaa actggcgcac
     1201 tcttccggca ttaacccggc gtatttgact atcccgatgc tgcaggcgtc aaacctcggt
     1261 cgtaccctgt cacccgtttc tggcgtagtc gttgcggttg ccgggatggc gaaaatctca
     1321 ccatttgaag tcgtaaaacg cacctcggtg ccagtgcttg ttgggctggt gattgttatc
     1381 gttgctacag agctgatggt gccaggaacg gcagcagcgg tcacaggcaa gtaaatagtc
     1441 atgccggagg cagtttcctc cggctttatc atgtcaccca ctgtggtata tgcgttgcgg
     1501 tctgccaact ttgccgtgaa caatttcagc aataatcaga tggcggctgg cgcaatattc
     1561 aagataacgc ctggcagtgg tgcggctgat ggtcagtgcc tgcgccaccg tttctgccgt
     1621 atgttgcaca cccggctctt taaacagttt tcgcaccgcg tttagcgtca aggggtcaat
     1681 gccggtcggt agctcgtcct taggttcacc gcgagcataa gcattaaaca tctcatcaat
     1741 ttgcttctgg ctggcgctat caatactttc cagcatatgt ttacgctggc ggaaacgggt
     1801 tagcgtttgc cccagccgtt cataggcaat tggcttaatg agataatcaa atacaccaca
     1861 acgtacggct tcagacaccg tttccatatc gctggctgca gtggtaaaca ccacgtcgcc
     1921 gggataatgc gcctgcacca gttcatgcag taaattaatc cctctaccgt caggaagata
     1981 gttatcgagc aagattagcc ccggcttaaa acgctcgatc atcattcggg cctgcgccag
     2041 gtttcccgcc agtaatatct gactgaatcc gggaatgtga cgaatatatt ccgcatgcat
     2101 ctctgccagc ggcgtttcgt cctcaacgat caatagggtt aatggagctg tcattaggtt
     2161 tcactttcgg aatatagatt gaaaataagg taccgcaggg atcattatct tcgagagtga
     2221 taacaccacc gcagcgcgtt acgtagctgg caatcaagta caacccaatg ccatgttcac
     2281 cgggctcgtc agcacgcgta ctgaccccct gctcaaatat tttgtctcgt agagactctg
     2341 gaacgccgca gccctgatcg gcgacttcaa tcaccacatc atcgccttca tcgctgagga
     2401 ataattcaac gatcttgttt ccttcatcgc tacgcaggct ggcttcgaag gcgttatcaa
     2461 gtaaattgcc aacaatggcg gcaaactcgg tgctatccag tcctggcggc agttgcgaaa
     2521 gctggctacc ggggacgatg atcattttta gccccagttc gcgggcgcgc tgcactttac
     2581 caaaaagcag ccccgccacc tggcgatcgg caaacgcctc gcgcaggctg tcaataagct
     2641 gttgctgggc ctgagactcc ccctgcacca tcgccagcac gcgatcatac tctttcatct
     2701 gcaacagacc attgagcgtc gacatccaat tgagatgctc gtgtcgcaat gtacgaaggc
     2761 tctcgacgta ttgttttatt tgcgtcagtt gcgcattgag ggtggaaatt tcgtctttac
     2821 tacgaaagct gataatggcc cccagcaaat catcacctga acgaatagct tcccggttgg
     2881 caataacgct cagaccgtta aagttcgcca ccacatcctg acgtttttca tcaatctgtt
     2941 cggtaaagaa atcggcaggc ctgaccactt ctgcaatggg tttacccaac cattgccgtc
     3001 caggggaact cagccccagc atctttcttg cgttacgatt gatggcggta atgtaaccgt
     3061 gcggatccac cgcaatcagc ccttcataaa ccgaactaaa cagcgcctct tgctgacgga
     3121 ccacgcgtgc gatttgcttt ggctccatgc ccatcatctg ccgacggata tgcgcggcca
     3181 ggaaccacga cagcaacatc agaatcccta acagcacgac aaacacgcct gccatcggta
     3241 ataaaaactc agcccgccag ctatcgattt tactcaccag gtagccaatc gacaccacgc
     3301 cgatgacttt tccatcgtca tcaaagattg gcgttttggc gcgcatcgcc atacccattg
     3361 accctttccc ggtaatgaag tagctctccc ctttctccag cgcgccctgt ttggtgaact
     3421 gcataggatg accaattttc tccggattag gatggtaaag gcggatcgag tgccggtcgc
     3481 caatcaccac ataatcaaaa tcggtatctc tttgtaattt gttagcgatg gtcgccagcc
     3541 gtttgtagtc acgcgttttt actgcgctga tgatactgtc attagaagca ataattttcg
     3601 cctgattcat tgccatgtcg cgtacatgaa gcgttaaata gtcctcaaaa ctggccgtaa
     3661 aatattgcgc cagggctgca atgacaaata ttgagaacac cagaatcagc aaaaagatac
     3721 gcagcggaaa tgccagtctt tggaaaaatg caaactgttt attctcgtta agctgcaaca
     3781 ttgttttcct tgcgggttgt cccgagcctc tttgacggat gtaattaaaa tgttgaatca
     3841 aaatacatag atattaatta taatcgtggc aaattt
//
LOCUS       DAZFKF010000228         3767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFKF010000228 DAZFKF010000000
VERSION     DAZFKF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.230"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..982
                     /locus_tag="ACHKA1_005294"
     CDS             <1..982
                     /locus_tag="ACHKA1_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO5682809.1"
                     /translation="NSVDLIVTDPPYFKVKPEGWDNQWTGDEDYLKWLDQCLAQFWRV
                     LKPAGSLYLFCGHRLASDIEIMMRERFSVLNHIIWAKPSGRWNGCNKESLRAYFPATE
                     RILFAEHYQGPYRPKDAGYEAKGRALKQHVMAPLIAYFRDARAALGITAKQIVDATGK
                     KNMVSHWFSASQWQLPNESDYLKLQSLFARVAEEKHQRGVLEKPHHQLLETYTSLNRQ
                     YAELQSEYKHLRRYFGVTAQVPYTDVWTHKPVQYYPGKHPCEKPAEMLQQIISASSRP
                     GDLVADFFMGSGSTVKAAMALGRRATGVELETERFEQTVREVQDLASQNG"
     gene            1023..1098
                     /locus_tag="ACHKA1_005295"
     tRNA            1023..1098
                     /locus_tag="ACHKA1_005295"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1056..1058,aa:Ile,seq:cat)
     gene            1355..1786
                     /locus_tag="ACHKA1_005296"
     CDS             1355..1786
                     /locus_tag="ACHKA1_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HFO5682810.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATITGQLDTNFKIGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            1783..1947
                     /locus_tag="ACHKA1_005297"
     CDS             1783..1947
                     /locus_tag="ACHKA1_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO5682811.1"
                     /translation="MTVKLRLAAVALLLFLVVMVDFTSRIMSVLADGVLVVGIVVVLF
                     PLVKKDMPGS"
     gene            2936..>3767
                     /locus_tag="ACHKA1_005298"
     CDS             2936..>3767
                     /locus_tag="ACHKA1_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO5682812.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDSRIKQLARRSTMTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGASAFTTGADGTYSESAGASENSLRWGVGK
                     PLYQDLVSRTKAALAKNPKNRLLAVVWMQGEGDAAVGTHAQHPGLFSAMVNQFRTELA
                     G"
BASE COUNT          878 a          907 c         1124 g          858 t
ORIGIN      
        1 aaactctgtc gatctgatag tcacagaccc gccatacttt aaagtgaagc ccgagggctg
       61 ggataaccag tggacggggg atgaggatta cctgaaatgg ctggaccagt gtcttgcgca
      121 gttctggcgg gtgctgaaac ctgccggaag tctttacctg ttctgtggtc atcgcctggc
      181 gtctgacatt gaaatcatga tgcgtgaacg cttcagtgtg ctgaaccata tcatctgggc
      241 aaagccgtcc ggacgctgga acgggtgcaa caaggaaagc ctgagggcgt atttccccgc
      301 cacagagcgc attctgttcg cggaacatta tcaggggccg tatcgtccga aagatgccgg
      361 gtatgaggcg aagggcaggg cactgaaaca gcatgtgatg gctccgctga ttgcttactt
      421 tcgtgatgcg cgtgctgccc tggggataac ggcaaaacag atagtggatg ccacaggaaa
      481 gaaaaacatg gtgtcgcact ggttcagtgc cagtcagtgg cagctaccga acgaaagcga
      541 ttatctgaaa ttacagtcgc tgtttgcccg ggtggcagaa gagaagcatc agcgcggtgt
      601 actggaaaag ccccaccacc agctgctgga gacgtatact tcactgaacc ggcagtatgc
      661 ggaactgcag agtgaatata agcatctgcg gcggtatttt ggcgtgacgg cgcaggtgcc
      721 gtacaccgat gtgtggacgc ataaaccggt gcagtactat cccgggaaac atccgtgcga
      781 aaaaccggca gaaatgctgc agcagataat cagcgcgagc agtcgtccgg gggacctggt
      841 tgcagatttt ttcatggggt cgggttcgac agtcaaagcc gcgatggcac tggggcgtcg
      901 tgcaactggt gttgagctgg agactgaacg ttttgagcag acggtcaggg aagttcagga
      961 tttagccagt cagaacggat gatattgcag gattagttac gtaccgttat tatcctgcac
     1021 ccggcccttt agctcagtgg tgagagcgag cgactcataa tcgccaggtc gctggttcaa
     1081 atccagcaag ggccaccata tcacataccg ccattagctc agccggacag agcaattgcc
     1141 ttctaagcaa tcggtcactg gttcgaatcc agtacaacgc gccagactta tttttcccgg
     1201 ctcgcttttg cgggcctttt ttgtatccgc gccacgcccg gcgcatatca accacagagc
     1261 ctttcggggg tgagcttacg gagtggtcag tgtgactttc tctgtgggca gatcgctccc
     1321 gggcgttggc tcacccaccc aaaggaacgt cacgatgttt ggtattttca aaaagaaaac
     1381 ccgcagagca gcagcggaaa ttaaaaagtt tgagaaacgt gatctggcac aggcggttat
     1441 taatgctgcc tatctggtgg cctatgcaga tggtgaatgt gaggcttcag agaaagcgaa
     1501 gatcgaacag gtattacgta accagcctgc gttgtccgcg tttacgtcag aaattaatgc
     1561 catcagtgcc acgatcacag gtcagcttga caccaacttt aaaattggtc gtcgagcggc
     1621 gttgcgtgaa attgaagatg tgaaacacga tacgcgtgaa gcggaagatg tgctggatgt
     1681 ggcggtggct attgctgaag cagatggtga aattgagccg gaagagcgta aggttctgga
     1741 agagattgct ggtgttcttg gcctgcgact ggagaaccac ctgtgacggt aaaactgcgt
     1801 ctggccgctg tggcactcct gctgtttctg gtggtgatgg tggatttcac cagcaggatc
     1861 atgtcggtgc tggcggatgg agtgctggtg gtcggtattg tggtggtgct ttttcctttg
     1921 gtgaaaaagg acatgccagg tagttagccg ggtatcagtc atgccccgaa aattttaaat
     1981 gtctcacaat tcagtcggtt gacagttgtc tggtttgcgg ggagtttgtt aaaagaaaca
     2041 ggcatggtga atccccctga gcggaggggc atatcagcga caggtgtttg gttacatccc
     2101 ttatcctttc tgtgcgggtt caggtgctga tactgaactc accgggaggc acccggcacc
     2161 atgcgcatga tgatacagat acgcagcgtc agcccctctc cggaggggct tttctgtgcc
     2221 ggatacatca cagtttctgg aaccttgggt actacagtat cagtcagggt gctatatttt
     2281 cagatgtgat gaaagcctgt cagcaggcag ggcgtatcgg aaatgaccca gtagagaaaa
     2341 cgttgactca gataccggtg ctgagttacc gggaaaccgg catcacatga ccgctatcct
     2401 tccaggcccg tccgctccgg tgggcctttt tactgcagaa aacaggttcc ccgttaaatg
     2461 ctatgttgct cacaattcag tcagttgaag gttgtcttcc cgactgagaa tttgttaaaa
     2521 aatttctgca tgatgaatcc ccctgtgcgg aggggcggct ggtgtacgga atggtctctg
     2581 atgatcgtta gcgagaatga cgcgggttcg gtggcaccgg gctgaactca ccgggaggca
     2641 cccggcatca tgtaatgaac ggtacatagc gctactccag cccctctccg gaggggcttt
     2701 cttatggata aaaaaagccc gcatggtgag atgcgggcag caaggaataa acaacaaaac
     2761 gtgaagtaat caatttttca gctggcgaat aatacccgac agtaatcact ctgcgcaact
     2821 gcgcggcctt tttcgtattg cgggctgtag tcttcctcct gccattgtcc tgtaacttcc
     2881 ggacttcagc ccgcccctca tctgactcac aacattatcc cgaccgggag gattcatggc
     2941 atttaaacac tatgacgtgg tcagggcggc gtcgccgtca gaccttgcgg agcgactgac
     3001 tcaaaaactg aaggaggggt ggcagccatt tggcagccct gtcgccatca cgccttatac
     3061 cctgatgcag gccattgcgg cggaaggtga tgtcaccaca cctgtgttgg tgaagccgtc
     3121 ggatggagaa ggcacagtaa tcagcgccac cagcgacccg gagtattact ttgttgtggt
     3181 tctggcaggg cagtcaaacg gcatgtcgta tggtgaaggc cttccgctgc cggagacata
     3241 tgaccgtccg gactcgcgta ttaagcagct ggcgcgccgc agtacgatga caccgggcgg
     3301 tgccgcctgt aagtataacg acattattcc ggcggaccat tgtctgcatg atgtgcagga
     3361 catgagccgt cttaaccatc cgaaagcgga cctgtcaaag gggcagtacg gaaccgtggg
     3421 gcaggggctg catatcgcca aaaaactgct gccgtttata ccggcgaatg cgggcattct
     3481 gctggttccg tgctgccgtg gtgcttcggc attcacaacg ggtgcagacg gcacatacag
     3541 cgaatcagcc ggtgcatcgg aaaattcact gcgctggggt gtgggtaagc cgctgtatca
     3601 ggatttggtg agccggacta aagccgcact ggcgaagaac ccgaaaaacc gtctgcttgc
     3661 ggtggtgtgg atgcagggag aaggtgatgc ggcagtgggg acgcatgcgc agcatccggg
     3721 gctgtttagt gcgatggtga atcagttcag aacggagctc gccggtc
//
LOCUS       DAZFKF010000229         3742 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFKF010000229 DAZFKF010000000
VERSION     DAZFKF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.231"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..130
                     /gene="ymgI"
                     /locus_tag="ACHKA1_005299"
     CDS             <1..130
                     /gene="ymgI"
                     /locus_tag="ACHKA1_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO5682813.1"
                     /translation="IIPIITATLILNYLNNSERSLVKQILIEDEIIVCATYLIPDI"
     gene            132..476
                     /gene="ymgG"
                     /locus_tag="ACHKA1_005300"
     CDS             132..476
                     /gene="ymgG"
                     /locus_tag="ACHKA1_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO5682814.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            480..815
                     /gene="ymgD"
                     /locus_tag="ACHKA1_005301"
     CDS             480..815
                     /gene="ymgD"
                     /locus_tag="ACHKA1_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO5682815.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(871..3519)
                     /locus_tag="ACHKA1_005302"
     CDS             complement(871..3519)
                     /locus_tag="ACHKA1_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECIAI39_1904 family autotransporter adhesin"
                     /protein_id="HFO5682816.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYADQFIHHDEYKHAVEIKDGEVK
                     DIDNALVITTGNGSYGVSVAGEGSNLAINNGVIVTTGGLYPSDNSSLSTALTASAIVS
                     EFGGAVILQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFSDGDNTFI
                     VTAGNHAVGVLACASPGSARACVSSLDEESTADTGNNENSAKAILDMAKGEITTHGTE
                     SYAAYANGTVVKSGDKLDYTNASVTLTNVDITTHGDNAHAIAARQGTVSFNQGEIYTT
                     GPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDVLSGSSLRSANEIL
                     YHKNETSNVTITDSEVSSAADVFINNIKGHLTVDTTNSKITGSANLSTDDNTHTCLSL
                     SDNSTWDIKADSTVSKLTVDNSTVYISRADGKDFEPTRLTITENYVGNNGVLHLRTEL
                     GDDNSATDKVVINGNTSGTTRVNVTNAGGSGAYTLNGIEIISVEGESNGEFIKDSRIF
                     AGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRPEAGSYVA
                     NLAAANTLFVMRLNDRAGETRYIDPVTEQVRSSRLWLRQIGGHNAWRDSNGQLRTTSH
                     RYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRGYSAGLYA
                     TWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYGFALNKSF
                     GLEAAKYTWIFQQQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRTFIRTQEK
                     NSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGARNLGEIKLGVNGNLNPAASV
                     WGNVGVQLGDNGYNDTAVMVGLKYKF"
BASE COUNT         1000 a          885 c          813 g         1044 t
ORIGIN      
        1 cataattcct atcatcacag ccacgctcat tttgaattat ctgaataata gcgagcgttc
       61 tttagttaag cagatcttaa ttgaagacga aattattgtg tgtgcaactt acttaatacc
      121 ggacatctaa gttgaagaaa aaaatacttg ccttcgggct tatctcagca ttgttctgct
      181 ccacgccagc gatggcggat atgaaccgca ccacgaaagg tgcattgcta ggcgcaggcg
      241 ttggtttact tactggcaat ggtgttaatg gcgtactgaa aggtgcagct gttggcgctg
      301 gtgttggtgc agtaacagaa aaaggccgcg acggtaaaaa tgcgcgtaaa ggtgcaaaag
      361 tgggtgccgc cgttggtgct gtcactggcg ttctgaccgg caatggcctc gaaggggcga
      421 ttaaaggtgc cgtgattggc ggtacgggtg gtgcaattct gggtaaaatg aaataaggaa
      481 tgatgatgaa aaaatttgct ttactggcgg gtctgtttgt tttcgcacca atgacttggg
      541 cgcaggacta caatattaaa aacggtttgc cgtcagaaac ctatattact tgcgcagaag
      601 ccaacgaaat ggcaaaaact gacagcgcac aggttgcaga aattgttgcg gtaatgggta
      661 atgccagcgt tgccagccgt gatttaaaaa ttgagcaatc tccggaactg agcgccaaag
      721 tggtcgaaaa actgaatcag gtttgcgcta aagacccgca aatgctgttg atcacggcaa
      781 ttgatgacac catgcgtgct atcggcaaaa aataatattt aatatgttac cgcgtcgatg
      841 aattcgacgc ggtattttag ctaacgggga tcagaactta tatttcaggc ccaccatcac
      901 tgcggtgtca ttgtagccat tatcacccag ctgcacgccc acattacccc agacgctggc
      961 cgccggattc agattgccat ttacgccaag tttaatttcc cccagattac gcgccccgtc
     1021 ttgttcgact ttcacaccgt tcattgagac agcaaaatct ttactgttat ggatccagtt
     1081 catttcaaca aaaggttcaa agtcgtcacc gtgcggaccg ctgtttttct cctgagtacg
     1141 aataaaggtg cggaaaccaa gtcgggtctg gatgttgtta tttgcgtcat tctcaatacg
     1201 tgagccattg gcttccgtat gcgcattatg atcgacgccc atccagatag cctgtgcctg
     1261 ttgctggaag atccacgtat atttcgccgc ttccagacca aaggatttat tcagggcaaa
     1321 gccgtaaccc gcttccagcg agacggttgc gcctttcgcg ctataggatt cataggctaa
     1381 ttcatcccct ttcaccgagt ttttaaacca gctatattgc gcccaggcgt caatgtatgc
     1441 gcctttttta ctaatgtcat cggcaaacca ggtggcatac agccctgcgc tatagcctct
     1501 gacactccct ttcgaacgat aatccgacac gctggaatga gttgagttgt agtcgcgggc
     1561 ataaccagcc atcactccca aacgccaact gtcactgtcg gtaaaaccac cggttaacag
     1621 atcggcccct aactgcgaga cgtagcgatg cgaggtcgtt ctcaactgtc cgttgctgtc
     1681 acgccaggca ttatgcccgc caatttgacg tagccaaagt cggcttgaac gcacctgttc
     1741 agttacagga tcgatgtagc gcgtttcacc cgcacggtcg ttcagacgca taacaaacag
     1801 agtgttagcg gctgccaggt tggcgacgta acttccagct tcggggcgca ggacgggggt
     1861 gggcgcaaca gtaggcgctg aacttcctcc ggagtttgtt tcaccgccgc tcgttgcctg
     1921 gaagttagtc agataccagt ttttattggt cgcttcggta ttacctcggg ttaatgaata
     1981 ttcgtaggca ccggcgaaaa tcctcgaatc cttaataaat tccccatttg attccccctc
     2041 aacgctgata atctctatcc catttaacgt ataagccccg ctgccgccag cattggtaac
     2101 gttaactcgg gttgttccag aggtatttcc attaataacg actttatccg tagctgaatt
     2161 atcatcaccc aattcagttc tgagatgcaa tacgccatta ttaccaacgt aattttcagt
     2221 tattgttaat cttgttggtt cgaaatcctt tccatctgcc cgggaaatat agaccgtact
     2281 attgtcaacc gtgagcttgc tcaccgttga gtcagcttta atatcccagg tactattatc
     2341 tgaaagcgac agacaggtat gagtgttatc atctgttgaa agattagctg aacccgttat
     2401 ttttgaatta gttgtatcga cggtcaaatg acctttaata ttattgataa aaacatctgc
     2461 agccgatgat acctcactat ccgtaatggt cacgttactc gtctcatttt tatggtagag
     2521 gatttcattt gctgaccgta gtgagctacc agataaaaca tctacagttg cttcctgacc
     2581 atttatggaa gactccagca caattccaga cccctgatgc gcgactgcag atgtattttt
     2641 cagcgtcacc gtgccgccat tataaatttt agcaatcgcg gcgtcaggac ccgttgtata
     2701 aatttctccc tggttaaatg aaactgtccc ctggcgagca gcgatggcat gagcattgtc
     2761 cccatgcgtg gtgatatcaa cattggttaa tgtaacgctg gcattggtat aatcaagctt
     2821 gtcaccagac ttgacgacag tgccattagc gtaagcggca taactttctg ttccgtgggt
     2881 tgttatctcc ccttttgcca tatccagtat tgctttggcg ctattttcat tatttcctgt
     2941 atcggcagta ctttcttcat caagagaaga tacacacgca cgagcgcttc cgggagatgc
     3001 acacgcaaga acgccgactg cgtgattacc cgcagtaaca ataaaagtat tatctccgtc
     3061 agaaaaagag ggggtaaccg aaccgtcaga gttgactctt attatggtat cagtattcag
     3121 gtttccactc gtttgactta gcaaaccaat tgagtaatcc cccgttgtga caatcgtgtt
     3181 tttaccctgc aaaataacag caccgccaaa ttcacttacg attgcgctag ctgttaatgc
     3241 tgtgcttaga gaactattgt cagaaggata taaaccgccc gttgttacaa tgacgccatt
     3301 gttaatggct aaattgctac cctctcctgc aacagataca ccgtaacttc cattacccgt
     3361 tgttataact agcgcattat caatatcttt tacttctcca tcttttattt ccacggcatg
     3421 tttatactca tcgtgatgaa taaactgatc agcatatgca ttacccaccg ccagagcaat
     3481 aagtcctaaa ctaaacccgg ggagtttttt cagtttcata gattaaccat ccattcattg
     3541 gattaacaaa cattcctggt caaataacct atgagttcag attaccaaaa cggagggtaa
     3601 agacaaaaac aaaagcacaa gaaatttaaa tagataaaca gcgacaatat ataaatgata
     3661 aataagtcgg cattatcatt tttcacctta ttttcatgac attgatcact ttgaggtgac
     3721 gattttgttt aacaaataac tc
//
LOCUS       DAZFKF010000230         3632 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFKF010000230 DAZFKF010000000
VERSION     DAZFKF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.232"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1181)
                     /gene="rhsD"
                     /locus_tag="ACHKA1_005303"
     CDS             complement(<1..1181)
                     /gene="rhsD"
                     /locus_tag="ACHKA1_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO5682817.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKATQPDGHTLARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLTYRREAAGDLAGGITGVTDDAGREFRLVLTTQAQRAEEARTSSLSSSDRSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPAAPLVRYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQDRIT
                     VT"
     gene            complement(1248..3356)
                     /locus_tag="ACHKA1_005304"
     CDS             complement(1248..3356)
                     /locus_tag="ACHKA1_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO5682818.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTCTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQIIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT          734 a         1108 c          982 g          808 t
ORIGIN      
        1 gtgacggtga tgcggtcctg ctcatactgg tagcggtaac tcaggcctgc cgggttaagc
       61 tgctccacca cccgccccgc atcgtcgtag cggtagcgca tctccggcct tcccgcgtaa
      121 cggtgtgcca ccatccgccc ggggtgctgc gcgtcatatg tgaaagcgcg cacctgcgta
      181 ttgctgcggt catataccgc cagcagttca ccggcttccg tatacgtgta ccgcaccagt
      241 ggcgcagcgg gcaggctctc cgggtatgcc gggtcgtgca tcagccacac cgccgaaagg
      301 cggatgcccc tgtcggggcc gtattcggta ccgggcagtg tgtcggggaa cgctgaggct
      361 gagagagggc gggaacggtc agaagaagat agcgaagagg tgcgggcctc ttccgcacgc
      421 tgcgcctgcg tggtcagcac cagacggaac tcccgcccgg catcgtccgt cacgccggtg
      481 attcccccgg ccaggtcacc ggcggcctca cgccggtacg tcagcgtccg cccgaagcgg
      541 tccgccatcc cggtaagcac ccggtacggc ggcagcggcg ctggcagtac gtcctcagca
      601 cccggcaccc gctcagacca ccccagtatc caccacggcc cctgtgcgct gttggtcgcc
      661 aggtaaagat gcgggcttaa ccggatatcc ggcggcagcg acgcccacag ccgtgccagc
      721 gtatggccgt ccggctgcgt tgccttacca ccgcgcacca gccacattga ctcgctgcgg
      781 ctgtacaccg cctcccccgg cagcagcggc tcaaagtgaa tgctccgccc gccgttgtcg
      841 ttgagtatca gtccgtcatc acgtagctgt aagcggatat cagaaggcgc tttccagccg
      901 gggccgaaaa cgcccaccgg tgcaggcgtc cgggtccggt agctgctgta ggtgcgggag
      961 agaatgaacg gcagcgggcc gggcagcgca aggtccgtct cgccgggcag caccttcgcc
     1021 cccagcagcg gatttaccgg gttgcccgaa gtcatcccgc ccggacacac cgagcacgcc
     1081 acgccagttg gcgcgccaat tcttacacct gccgaaccct ggacaatggg accgccatac
     1141 tgggtcatat ctccctgacg cgccgctggt tttccgctca tgattcaatt ccttcttctg
     1201 tttattttta atgcttgttt gttactgaat accgaacagg tacgggatca gtactctctc
     1261 atatccggcg tatcctgctt ttcatcaggg gtcttcggtg gggatttcga ggctgcacag
     1321 ttccagttaa tcaacagtga gtcaccatta acctgctcct taccctgcaa atgaattttt
     1381 gtgccgttga gaaaaatttg tccgtccttc gtcagcacca gccttgcctt accacagcag
     1441 agctccagat gctcaccggc ggagtaaatc gcggtctgcc cggtatcatc ctttttcgtt
     1501 ttaccaacgg tgaaggtaac gttatttccg actttgacgt cataacccag cccgaccctc
     1561 aacgatttat gcaaaccgat ctgcgaggac tgcatcagag ccaccgaggt gttcataatg
     1621 ccacctaccg tcgtctgata cgccacgccc acggtcagcg cacgaacaag tccaatcgtt
     1681 tccacctgaa cagaacccac gttaatgatc tggttactgc ccaccgtctc tatctggtta
     1741 acgcccaccg tctgtatctg attgttggta accgcaatta tctgattgtt gccgatggtc
     1801 tcagcatggt tatttatcac atccgtggtg cggttattca gcacctcggt gttcatgttc
     1861 ttctgcgcgt ggatgtagac ctgttctttc cctgtcgcat cgtcaaactt cagttcatta
     1921 aacccgctgc ccttataggt tttcgaacga atggtcatct gcgtctttgt tcccggcagg
     1981 ctgccgggtg tgcggttttc ctggtggtag gtgcgcccca taatgatcgg ctggtccgga
     2041 tcgccgttga ggaagtccac aatcacctcc tgacccacgc gcggtatcgc aaggttaccg
     2101 aatccggtgc ctgcccacgc ctgtgccaca cggatccagc atgaactgtc ctggtttgac
     2161 gggttataac ggtcccagtt aaatttcacc cgcacgcggc catgctcatc acagaagatt
     2221 tcctcgcctg ccggtcccgt cacgacggcg ctctgcgggc cgtccaccag cggtttcagc
     2281 aacggctgtg gtcgccatgt tctgtctgcc ggtattaccg caaaatggtt atccagcgtg
     2341 gtgcctgaac ccctgcgtcc cggtaccgcc tgcggctgtt cgccgtgaag atcgctcgcc
     2401 accacctgcc attcccggtt caggttcgcc tgcggatgcc ccgtcagcac aattcgccgt
     2461 cccggccata tctccggcga acggcttgtt ccgcgcgcca cttctgcgtt gttgcgccag
     2521 ccatccatct gccagcgggc aaagttctgc ccgtgggcac ccttgaaacg tccggggtag
     2581 tcatacactt catactgtgt gcgctggtaa tcctggtact ggccttcctg atcaaaacgc
     2641 cctgcccagc cgggtcgttt aaaggtgtag tccttggtca ccacggaaga agggcggatt
     2701 tgtgcgctgt agcggaactg gctgatgcag agggtgctta cctcggtaca ggtgttcgga
     2761 ttccagggga tctcaaagga ctccggcaga taacgcacgg tgtcgcacag gaccaggctc
     2821 tggtcggtac ttttttgcgc gtgctcctca taaaagaaga tgccttcctc cgccgccatc
     2881 cggcacagga aatcgtaatc agtctcaccg tactggacac aaaactcacg ggaaggatgt
     2941 ggctcgctga acagcgggct ccactcggtc accccgtttt cctgcaatat cgtgccgagg
     3001 atgctttcga tgtcctcatt ctggaagata cggaagttct ggcgcagccc tgtgcgccac
     3061 agcggtgggc acaccttcat gctgtacagc atctggtttt tgtcgttctc ccccagttca
     3121 aaccaggtca ccacaccttt cacccggcgc tgtacgtcat cgccctgcca taccgtcagg
     3181 taggccattt tgtccagcac ctgcgcaaat tcaagggaaa gaaactgctg actgaccaga
     3241 gaaaggtcga gggaaaaaag tgaagagagt gactgggtga gatgaaagga aaccaccgca
     3301 aaagcatccg gtggcaggcc gtccacttcc agcgtgaaac gtaatccggt tgacatagca
     3361 acctccgtga aaatgaataa taatccgcac atattgcatg tacaacatgt cgggcggatg
     3421 agataagcga gtcgacatct ggcgttaccg gggaatatca gaaaaatgtc atggttatgt
     3481 gatgatttag tctgggattt tacttcagag aaacgggtgt ctgtttagtc aacaaaataa
     3541 tcacaaacgc aacggatggg ttctcgacgt tattcacgac aggctcgtac attacagcga
     3601 gtaataaaaa acctgatatg caaagtcaaa cc
//
LOCUS       DAZFKF010000231         3459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFKF010000231 DAZFKF010000000
VERSION     DAZFKF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.233"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..3143
                     /locus_tag="ACHKA1_005305"
     CDS             <1..3143
                     /locus_tag="ACHKA1_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5682819.1"
                     /translation="YDAAKGVGRIQQDDQQFVMHGNLNGNLNAGKNLYFTGANGIIDL
                     KDNVNQGAGYLQFADDYTVTTSNASSWFGGGIIVNSGTTVKWGINGVSGDDLHKVGDG
                     TLIINGTGKNEGGLKIGAGTVILEQKAKNNDSTAFSSINISGGNSRVKLSGDNQIIPD
                     NVSWGFRGGYLDINGKNTEFSRLQAVDYGAAIINSSTEKSLLTLNLSPLKKDEIAVSV
                     KALDMNAIFHGGYGTTGDLYKTTFYGPTQYYLLKKPKFGSVLMGSLKNTSEWQFAGTD
                     LNQAVDMAKNNKLTSSAQASYLYHGKLLGNMDIVIPELTGNDILTLDGSVSISGDMSK
                     QDGALIFQGHPVIHAGQTVSASQSDWENREFSLNNLNLNNADFSLSRNAFMNGNIRAV
                     NQSTVIIGGDTVFTDKNDGTGNDVISVEGKSAAAGTSSYTGHITLEQKSALDIRDNFR
                     GGVTSEDSHINVSSSSVLFSDASSFINSSLNIHKGGALTAQGGLFTSGSIDIGDASLL
                     LTGTPVNSDDAAFLPTINMADGGFKLMSDSSVLKARDQASVVGDIISDKQATISFGTE
                     SGKEGILSEKASRGVAVGLLSGFNTAYRGAIHAPSASATVNNTWWQLTGDSSLRSLKN
                     TGSMTYFTGSAANKAFHTLTVDELTTNGTAYAMRTDLKNADKLVVNKKLSGKDNILLV
                     DFLNKPTGEKLDIELVSAPGNSSKDVFKGSEQAIGFSNVTPVITAIDAGDKTTWNLTG
                     YRMAENPAATQSASGLASVGYKSFLSEVNNLNKRMGDLRDINGEAGAWARIMSGTGSA
                     GGGFSDNHTHVQVGVDKKHELDGLDLFTGFTVTHTDSSASADAFKGKTKSVGAGLYAS
                     AMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYSTHSWYAGAEAGYRCHVTEDTWIE
                     PQAELVYGAVSGKQFAWKDQGMHLSMKDRDYNPLIGRTGVDVGKSFSGKDWKVTARAG
                     LGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSVGLNAEIRDNVRFGLEFEKSAFG
                     KYNVDNAVNANFRYSF"
BASE COUNT          988 a          722 c          876 g          873 t
ORIGIN      
        1 catatgatgc agccaaaggc gtgggccgta ttcagcagga tgaccaacag tttgtgatgc
       61 atggtaacct gaatggtaat ctgaatgcag gtaaaaatct ttattttaca ggcgcaaatg
      121 gcattattga tttaaaagat aatgttaatc agggagccgg atatcttcag tttgcggatg
      181 attatacagt aacaacgagc aatgccagtt catggttcgg aggaggcatt attgtcaatt
      241 cgggtacaac tgtaaaatgg ggtataaacg gggtttccgg agatgacctt cataaagttg
      301 gggacggaac gcttattatt aatggcacag ggaaaaatga agggggactg aagattgggg
      361 ctggtactgt aatccttgaa caaaaagcga aaaacaacga ttcaacggct ttttcttcca
      421 ttaacatcag tgggggaaac tcgcgcgtta aactctcagg agataatcag attatacctg
      481 ataatgtttc ctggggattc cggggtggat atttagatat aaacggaaaa aacacagagt
      541 tctcccgctt acaggctgtt gattatgggg ctgcaattat taacagcagc acagagaagt
      601 cattattgac gttaaatctg tcaccattga aaaaagatga aatagctgtg agcgtgaagg
      661 cgttagatat gaacgccatc tttcatggag gatacgggac aaccggcgat ttgtataaaa
      721 caacttttta cggtccgaca cagtattatc tgctgaaaaa gcccaaattt ggcagtgtgc
      781 tgatggggtc actgaaaaac accagcgaat ggcaatttgc aggaacagac ctgaatcagg
      841 ccgttgatat ggcaaagaat aataaactga catcaagtgc acaggcttca tatctttatc
      901 acggtaagct cctggggaat atggatattg tcattccgga gcttacaggc aatgatattt
      961 taacgcttga tggctctgtc agtatatcag gagatatgtc aaaacaggac ggtgctctta
     1021 tcttccaggg acacccggtt attcatgcag ggcaaactgt ttctgcatcg cagagtgact
     1081 gggagaacag ggagttctca ctcaacaatc tgaatcttaa taatgcggac ttcagtctgt
     1141 cccgtaatgc atttatgaac gggaatatca gggccgttaa ccagagcact gttattatcg
     1201 gcggagatac agtctttact gataaaaatg acggaacagg taatgatgtc atcagtgttg
     1261 aagggaaatc tgctgccgca ggaacatcct cctatacagg gcatatcact ctggagcaaa
     1321 aatcagcact ggatatccgc gataattttc gtggcggggt tacgtctgaa gacagtcata
     1381 tcaatgtttc ttcatcttca gtcctgttct cagatgcatc gtcatttata aacagctccc
     1441 tgaatattca taaaggaggt gcgctgaccg ctcagggagg gctgtttaca agtggaagca
     1501 ttgatattgg tgacgcttcc cttctgctta ccggtacacc agtgaattca gatgatgctg
     1561 cttttttacc gaccatcaat atggctgatg gcggatttaa actgatgtct gattcatcag
     1621 tactgaaagc cagagaccag gcatctgttg ttggtgatat tatttctgat aaacaggcca
     1681 caatcagctt cggaactgaa tcaggtaaag agggcatatt gtctgagaag gcatcccggg
     1741 gagttgcggt cggattactg agtggtttta atacggcata ccgcggtgca attcatgccc
     1801 cgtctgcatc tgccactgtg aacaatacct ggtggcaact gacaggagac tcctcacttc
     1861 gctcgttaaa aaataccgga agcatgacat attttacagg aagtgcagcg aataaagcat
     1921 tccatacact gacggttgat gagctgacga cgaatggcac tgcgtatgcc atgcgtacgg
     1981 acctgaaaaa tgcggataag ctggtagtaa ataaaaagct gtcaggtaag gacaatattc
     2041 tgctggttga ttttctgaac aaacccaccg gagaaaaact ggatattgaa ctggtgagtg
     2101 caccggggaa cagcagtaag gatgttttta aaggaagtga acaggcaata ggtttcagca
     2161 atgtcacacc tgttattaca gctatagacg ccggagataa aacaacatgg aacctgaccg
     2221 ggtacaggat ggcagaaaat cctgccgcaa cccaaagtgc ctcaggcctt gcatctgtgg
     2281 ggtacaaatc atttttgagt gaggtcaaca acctgaataa acgtatgggt gacctgcgtg
     2341 acatcaatgg tgaagctggc gcatgggcac gtatcatgag cggaaccggc tctgccggtg
     2401 gtggtttcag tgacaaccac acacatgttc aggtcggtgt cgacaaaaaa catgagctgg
     2461 acggactgga tttgtttacc ggcttcactg tcacacacac tgacagcagt gcctccgctg
     2521 atgctttcaa aggtaaaacg aagtctgtgg gggccggact ctatgcttct gccatgtttg
     2581 attccggtgc ctatatcgac ctgattggta agtatgttca tcatgataat gagtacacgg
     2641 caacctttgc cggactcgga acccgtgatt acagtacgca ttcatggtat gccggtgctg
     2701 aagcaggcta ccgctgtcat gtcactgagg atacctggat tgagccacag gcagaactgg
     2761 tttacggtgc tgtatccggt aaacagtttg catggaagga ccaggggatg catctgtcca
     2821 tgaaggacag ggactacaat ccgctgattg gtcgtaccgg tgtggatgtg ggtaaatcct
     2881 tctcaggtaa ggactggaaa gtgacagccc gtgccggtct gggctaccag ttcgacctgc
     2941 tggctaacgg cgaaactgta ttgcgggacg catcaggtga aaaacgtatc aaaggtgaaa
     3001 aggacagccg tatgctgatg tccgttggcc tgaatgcaga aatcagggat aacgtccgct
     3061 ttggactgga gtttgagaaa tccgcctttg gtaagtacaa cgttgataat gctgtcaacg
     3121 ctaacttccg ttactcgttc tgatactccg tattcaaaag ggcctgaggg ccctttatgc
     3181 tcaggacctg ccgcccgggt atacatttcc ggggtatcag tcgatttgtt tacttaaccg
     3241 ccatgaccaa agccggcagc aatgtcggct ttttacgtcc atatattcca cttatccgga
     3301 actgtgacac agaacctgaa tacaggattg atcaccggtg cccacaaatg gcacattttc
     3361 agtaagattc tgaatgttac attccccctt catgtgtgac acagctgtca cagagaacga
     3421 aacggaaagc acatgaaaaa cataaaaact cacaccgga
//
LOCUS       DAZFKF010000232         3434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFKF010000232 DAZFKF010000000
VERSION     DAZFKF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.234"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1186
                     /locus_tag="ACHKA1_005306"
     CDS             <1..1186
                     /locus_tag="ACHKA1_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFO5682820.1"
                     /translation="VARLDVLTTLQAEVQRAVDEGISQKAFISTLTPRLQKLGWWGKQ
                     IVVDSAGNAEEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDS
                     RTRPAHSALNGLVFRYDDPFWNTHYPPNGWNCRCRVRPLSQVRLDAMGLSVSSGQDHL
                     STRNVEAGVDKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIE
                     VKSPALREMVVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESR
                     TGTPPARLLAMSGKNVLHADSMKHQNDGIALTPEDFGRLPAMLARPKAVLWDKRHNNL
                     MYIVENQDGSVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGD
                     ID"
     gene            complement(1188..1267)
                     /locus_tag="ACHKA1_005307"
     tRNA            complement(1188..1267)
                     /locus_tag="ACHKA1_005307"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            1308..1781
                     /locus_tag="ACHKA1_005308"
     CDS             1308..1781
                     /locus_tag="ACHKA1_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO5682821.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKGLARSVASSLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            1958..3082
                     /locus_tag="ACHKA1_005309"
     CDS             1958..3082
                     /locus_tag="ACHKA1_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO5682822.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIEGHPAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDESTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQLLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTGLQTD
                     TVTVPDKKTATAVLSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            3082..>3434
                     /locus_tag="ACHKA1_005310"
     CDS             3082..>3434
                     /locus_tag="ACHKA1_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO5682823.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAINRDDFEDDNLGIYSP
                     LFQEMGRAAAAQPDEL"
BASE COUNT          807 a          935 c          995 g          697 t
ORIGIN      
        1 agtggcacgc ctggacgtac tgaccacact acaggcggaa gtgcagcgtg ctgttgatga
       61 ggggatttca cagaaagcat ttatcagcac gctgacaccc cgcctgcaaa agctgggatg
      121 gtggggaaaa cagattgtgg tggacagcgc cggtaacgca gaagaagtgc agctgggcag
      181 tccccgccgt ctggcgctga tttacaacgt gaatacccgt gtggcttaca atgccgggcg
      241 ttacacgcag atgatgaaca acacggacac gcatccgttc tggcagtatg tggcggtgat
      301 ggacagccgc acccgcccgg cgcattccgc tcttaacggt ctggtattcc gctatgacga
      361 tccgttctgg aatacacact acccacccaa tggctggaac tgtcgctgcc gtgtccggcc
      421 attgtctcag gtccgtctgg atgcaatggg gttatccgtt tcatccggtc aggatcatct
      481 ctccacccgc aatgttgagg ctggcgtgga taagcagacc ggggaagtca gagaaatgcc
      541 ggtgaccaca tattcagatg gcaccagaac catgacaccg gatgtgggct ggtcatataa
      601 ccccggttcg gcggcgttcg gcacagacca ggcgctgatc cgtaaactga tcgaggtgaa
      661 aagcccggcg ctgcgggaaa tggtggttca ggagatgaac aacagcccgg agcggcagct
      721 ggcgttccgc atctgggcaa aaaacatcat gaaaacccgg cgaggcggta acgatatccg
      781 cacgctgggc tttatgaccg aaagcattgc gcaggcggtg gaaagccgga cgggaacgcc
      841 accggcccgc ctgctggcga tgagcggtaa aaatgtgctc catgcggaca gcatgaaaca
      901 tcagaatgac ggtatcgccc tgacgccgga ggatttcggg cgcttaccgg caatgctggc
      961 aaggccaaaa gcggtactgt gggacaaacg gcacaacaat ctgatgtata tcgtggaaaa
     1021 tcaggatggc agtgtgcaga ttgccgttaa tgcaccgtac agcctgaagc gtcagccgga
     1081 taaactggat gtgattgtta atgcttaccg ggttatcaat atggataagc tgaaatcgga
     1141 tatccggggc ggaatgcttg aggtactgga aggagatatt gattaacggt cagtggcggg
     1201 ggtcgaaccc gcatacatga tccacccgga ggcaggcacc gactttacca gttaagcgta
     1261 cactgaccgc ctgtttattt tatattcatc ctttatgcgg aggcaatatg tcatccattg
     1321 atgccgctgt cgttgttgac gttacgcgtc tccagcgggt ctttgcccgg cttcagtttg
     1381 tgggtggcgg gaaaggcctg gcccgcagtg tggcgtccag ccttctgtca tcgtcagaaa
     1441 tggcgtttga acaggaaaaa gagccggacg gcgaacgctg gcatgactgg tcagatcctt
     1501 accgcaagtg gcgtacccgt aagggataca tgcccggcaa aatcctgaca ctgaacggcg
     1561 atctggcccg acgcctgacc acggattatg gcgacacctg ggcgctgatt ggatcaaatg
     1621 agccttatgc ggccattcat cagtggggtg gcctgcccgg tatgccgccg ggaccggcgg
     1681 ccattggtgc acgtccgtat atgggctttg atcaggtggc agagcaggag atcatggacg
     1741 aaatcagaaa acgctttaaa aaggccacag aaacgcctta attgtttaag gtatgcaaat
     1801 gcattacctt acccccttca ggcgcgtgtc gtgattttct aacctgaatt taacgggctt
     1861 taaaatctgc gtgaagcgcc tttgtatttt tcccctgaaa gcacttcctg aaatcccgca
     1921 aaacccgccc tgaaaattcc cggcctatgc tgccggaatg aagacgaaaa acacgcccaa
     1981 acttgcttat gccattctga atgccatcag cctgtccgca gacggggacg gtgactggtg
     2041 tcagatcatg ccagccggtc gggtaaaggc ccgtgacggt cgcccggaaa aaccggccga
     2101 gggctggctg atcaaccatg cagccgttga gcgcatggtc tcccgtgttg tggcgctcaa
     2161 tcagccggtg aaaattgact acaaccacca gaccctgatt gagggacatc cggcaccggc
     2221 tgccggtttt gtgatggcct caccggagaa ttttcgcttc agtgaagagc gaggtttcga
     2281 ggtgcgcccg aagtggaacc ctccggccct tgaacatctg cgtaataacg aattcccctg
     2341 gttttcaccg gtgattggct atgacgagag caccggcgaa cctgtcgaac ttcggatgct
     2401 ggctattacc ggtgaccccg gtctgaccgg catgaatcct gtcgccgcac tgtcggcgga
     2461 tgacctttat aacgccttaa accctccttt aaaggatacc tccatgaatg agcaattacg
     2521 ccagttgctg acggcgctcg gtctgaccgt ggctgacggt gacgaattta cgccggagct
     2581 gggcacggca gcgctgtctg ccctcaccgg gatcaaaacc cgtgcggatg cacacgacaa
     2641 cctgaaaaca caggtcgcca gtctgtcggc agagctggaa actgcaaaag gcacaccggc
     2701 tggcggcagt attgacctga cgaaatacgt gcccgttgaa acgtataacg ccctgcgtac
     2761 cgaatacgtt gcgctgtcgg cgcagcacgg cagcaccacg ctggagcagt tgctggacaa
     2821 ggccgagtcc gaaggacgca tcttcaaaag tgaacgcggc tatctggaac aactgggcgg
     2881 gcagattggc gtggcggcac tctctgcaca gcttgacgcc cgccagccgg ttgcggccct
     2941 gaccggcctt cagaccgaca ccgtgaccgt gccggataaa aagactgcca ccgctgtgct
     3001 gtcggctgaa gatatcgcgg ccgccaaact gctgggtaaa accgaagccg agttcctgaa
     3061 aatgaaagag gaaatgcaat aatgcctacc ccgattacac cggcgatgat caccgccctg
     3121 atgacgggct accgttctga ttttcaggcc gggatgtcca tggccccgtc gcagtacaaa
     3181 aaaattgcga tgaccgtgcc ctccacatcg aaatccaaca cctacggctg gctggggcag
     3241 ttcccgcagt tccgtgagtg gatcggctcc cgcgtcatcg agaagatgaa ggcctatggc
     3301 tatgccatcg tgaacaaaac ctttgaaggc accgtcgcca ttaaccgcga tgacttcgaa
     3361 gacgataacc tcggtattta ttccccgttg ttccaggaga tggggcgcgc tgctgcggca
     3421 caaccggatg aact
//
LOCUS       DAZFKF010000233         3425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFKF010000233 DAZFKF010000000
VERSION     DAZFKF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.235"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1180
                     /locus_tag="ACHKA1_005311"
     CDS             <1..1180
                     /locus_tag="ACHKA1_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFO5682824.1"
                     /translation="VARLDVLTTLQVEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQ
                     IVVDSAGNAEEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDS
                     RTRPAHSALNGLVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHL
                     STRNVEAGVDKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIE
                     VKSPALREMVVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESR
                     TGTPPARLLAMSGKNVLHADSVKHQNDGIALTPEDFGRLPAMLAAPDAVLWDNVHQNL
                     LYITETRDGTAKIAVNAPYGVKRQPDKLDVVINAYRVNKFDIEKAIEGGKLELLEGKL
                     "
     gene            complement(1180..1260)
                     /locus_tag="ACHKA1_005312"
     tRNA            complement(1180..1260)
                     /locus_tag="ACHKA1_005312"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            1299..1772
                     /locus_tag="ACHKA1_005313"
     CDS             1299..1772
                     /locus_tag="ACHKA1_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO5682825.1"
                     /translation="MSSIDAAVVVDVARLQRVFARLQFVGGGKDLARSVASSLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMAEIRKRFKKATETP"
     gene            1949..3073
                     /locus_tag="ACHKA1_005314"
     CDS             1949..3073
                     /locus_tag="ACHKA1_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO5682826.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINRATVERMVSRVVALNQPVKIDYNHQTLIKGHQAPAAGFVTASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDENTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPIETYNALRTEYVALSAQ
                     HGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVTALSAQLDARQPVAALTGLQTD
                     TVTVPDKKTATASLSAEDIAAAHLLGKTEAEFLKMKEEMQ"
     gene            3073..>3425
                     /locus_tag="ACHKA1_005315"
     CDS             3073..>3425
                     /locus_tag="ACHKA1_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO5682827.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAINRDDFEDDNLGIYSP
                     LFQEMGRAAAAQPDEL"
BASE COUNT          810 a          933 c          985 g          697 t
ORIGIN      
        1 agtggcacgc ctggacgtac tgaccacact acaggtggaa gtgcagcgtg ctgttgatga
       61 ggggatttca cagaaagcat ttatccgcac gctgacaccc cgcctgcaaa agctgggatg
      121 gtggggaaag cagattgtgg tggacagcgc agggaacgcg gaagaagtgc aactgggcag
      181 tccccgtcgt ctggcgctga tttacaacgt gaacacccgc gtggcttaca atgccgggcg
      241 ttacacgcag atgatgaaca acacggacac gcatccgttc tggcagtatg tggcggtgat
      301 ggacagccgc acccgcccgg cgcattccgc ccttaacggg ctggtattcc gctatgacga
      361 tccgttctgg aatacacact acccgcccaa tggctggaac tgtcgctgcc gtgtccggcc
      421 attgtctcag gcccgtctgg atgcaatggg actctctgtt tcatccggtc aggatcatct
      481 ctccacccgc aatgttgagg ctggcgtgga taagcagacc ggggaagtca gagaaatgcc
      541 ggtgaccaca tattcagatg gcaccagaac catgacaccg gatgtgggct ggtcatataa
      601 ccccggttcg gcggcgttcg gcacagacca ggcgctgatc cgtaaactga tcgaggtgaa
      661 aagcccggcg ttacgggaaa tggtggttca ggagatgaac aacagcccgg agcggcagct
      721 ggcgttccgc atctgggcaa aaaacatcat gaaaacccgg cgaggcggta acgatatccg
      781 cacgctgggc tttatgaccg aaagtattgc gcaggcggtg gaaagccgga cgggaacgcc
      841 accggcccgc ctgctggcga tgagcggtaa aaatgtgctc catgcggaca gcgtgaaaca
      901 tcagaatgac ggcattgccc tgacgccgga ggatttcggg cgattaccgg caatgctggc
      961 cgcccccgat gccgtgctgt gggataatgt ccaccagaac ctgctttaca tcactgaaac
     1021 ccgtgacgga acggcaaaga ttgccgtcaa tgcgccttat ggtgtgaagc gccagccgga
     1081 taagctggat gtggtaatta atgcttaccg ggtaaataaa tttgatattg aaaaagcaat
     1141 agagggcgga aagctggaac tactggaagg taaattgtaa gccggtgacg ggggtcgaac
     1201 ccgcataaac atatgacccc tgagggttta tgccgcttta ccagttaaga gtacaccggc
     1261 ctgtatttat tttaatcaga tagttttaag gaggcaacat gtcatccatc gatgcagccg
     1321 tggtggtgga cgttgcgcgt ctccagcggg tctttgcccg gcttcagttt gtgggtggtg
     1381 gaaaagacct ggcccgcagt gtggcgtcca gccttctgtc atcctcagaa atggcgtttg
     1441 aacaggaaaa agagccggac ggcgaacgct ggcatgactg gtcagatcct taccgcaagt
     1501 ggcgtacccg taagggatac atgcccggca aaatcctgac gctgaacggc gatctggccc
     1561 gacgcctgac cacggattat ggcgatacct gggcgctgat tggatcaaat gagccttatg
     1621 cggccattca tcagtggggt ggcctgccag gtatgccgcc tggaccggcg gccattggtg
     1681 cacgtccgta tatgggcttt gatcaggtgg cagagcagga gatcatggcc gaaatcagaa
     1741 aacgctttaa aaaggccaca gaaacgcctt aattgtttaa ggtatgcaaa tgcattacct
     1801 tacccccttc aggcgcgtgt cgtgattttc taacctgtat ttaacgggct ttaaaatctg
     1861 cgtgaagcgc ctttgtattt ttcccctgga agcacttcct gaaatcccgc aaaacccgcc
     1921 ctgaaaattc ccggcttatg ctgccggaat gaagacgaaa aacacgccca aacttgctta
     1981 tgccattctg aatgccatca gcctgtccgc agacggggac ggtgactggt gtcagatcat
     2041 gccagccggt cgggtaaagg cccgtgacgg tcgcccggaa aaaccggctg aaggctggct
     2101 gattaaccgt gcgaccgttg agcgcatggt atcccgggtt gtggcgctca atcagccggt
     2161 aaaaattgac tacaaccacc agaccctgat taaggggcat caggcaccgg ctgccggttt
     2221 tgtgacggct tcaccggaaa acttccgctt cagtgaagag cgtggcttcg aggtgcgccc
     2281 gaagtggaac cctccggccc ttgaacatct gcgtaataac gaattcccct ggttttcacc
     2341 ggtgattggc tatgacgaga acaccggcga acctgtcgaa cttcggatgc tggctattac
     2401 cggtgacccc ggtctgaccg gcatgaatcc tgtcgccgcg ctgtcggcgg atgaccttta
     2461 taacgcctta aaccctcctt taaaggatac ctccatgaat gagcaattac gccagttgct
     2521 gacggcgctc ggtctgaccg tggctgacgg tgacgaattt acgccggagc tgggcacggc
     2581 ggcgctgtct gccctcaccg ggatcaaaac ccgtgcggat gcacacgaca acctgaaaac
     2641 acaggtcgcc agtctgtcgg cagagctgga aaccgcaaaa ggtacaccgg ctggcggcag
     2701 tattgatctg acgaaatatg tgcccattga aacgtataac gccctgcgta ccgaatacgt
     2761 tgcgctgtca gcacagcacg gcagcaccac gctggagcag gtgctggaca aggccgagtc
     2821 cgaagggcgc atcttcaaaa gcgaacgcgg ctatctggaa cagctgggtg ggcagattgg
     2881 tgttactgca ctttctgcac agcttgacgc ccgacagccg gttgcagccc tgaccggcct
     2941 tcagaccgac accgtgaccg tgccggataa aaagaccgcc accgcctcgc tgtcggctga
     3001 agatatcgcc gccgcccatc ttctgggtaa aaccgaagcc gaattcctga aaatgaaaga
     3061 ggaaatgcaa taatgcctac cccgattaca ccggcgatga tcaccgccct gatgacgggc
     3121 taccgttctg attttcaggc cgggatgtcc atggccccgt cgcagtacaa aaaaattgcg
     3181 atgaccgtgc cctccacctc gaaatccaac acctacggct ggctggggca gttcccgcag
     3241 ttccgtgagt ggatcggctc ccgcgtcatc gagaagatga aggcctatgg ctatgccatc
     3301 gtgaacaaaa cctttgaagg caccgtcgcc attaaccgcg atgacttcga agacgataac
     3361 ctcggtattt attccccgtt gttccaggag atggggcgcg ctgctgcggc acaaccggat
     3421 gaact
//
LOCUS       DAZFKF010000234         3421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFKF010000234 DAZFKF010000000
VERSION     DAZFKF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.236"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            127..1278
                     /gene="yiaY"
                     /locus_tag="ACHKA1_005316"
     CDS             127..1278
                     /gene="yiaY"
                     /locus_tag="ACHKA1_005316"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO5682828.1"
                     /translation="MAASTFFIPSVNVIGADSLADAMNMMAEYGFRRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSIIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1443..2981
                     /gene="aldB"
                     /locus_tag="ACHKA1_005317"
     CDS             1443..2981
                     /gene="aldB"
                     /locus_tag="ACHKA1_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO5682829.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAYTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
BASE COUNT          854 a          831 c          909 g          827 t
ORIGIN      
        1 cattgtttaa tgaatacaat gtgcttttta ttagattaat tttgctaaac aataatactg
       61 atcatatttt cgaatgaaaa tcctcagtaa gctgcccgcc cttttttaca ctttcaggag
      121 tgtgttatgg cagcttcaac cttttttatt ccttctgtga atgtcatcgg cgctgattca
      181 ttggctgatg caatgaatat gatggcagaa tatgggtttc gccgtacatt gattgtcact
      241 gacagtatgt taacgaaatt aggtatggcg ggcgatgtgc aaaaagcact ggaagaacgc
      301 aatattttta gcattattta tgatggtacc caacctaacc ccaccacgga aaacgtcgcc
      361 gcaggtttga aattacttaa agaaaataat tgcgatagcg tgatctcctt aggcggtggt
      421 tctccgcacg actgcgcaaa aggtattgcg ctggtggcag ccaatggcgg cgatattcgc
      481 gattacgaag gcgttgaccg ctctgcaaaa ccgcagctgc cgatgatcgc catcaatacc
      541 acggcgggta cggcctctga aatgacccgt ttctgcatca tcactgacga agcgcgtcat
      601 atcaaaatgg cgattgttga taaacatgtc actccgctgc tttctgtcaa tgactcctcg
      661 ctgatgatcg gtatgccgaa gtcactgacc gccgcaacgg gtatggatgc cttaacgcac
      721 gctatcgaag cgtatgtttc tattgccgcc acgccgatca ctgacgcttg tgcactgaaa
      781 gccgtgacca tgattgccga aaacctgccg ttagccgttg aagatggcag taatgcgaaa
      841 gcgcgtgaag caatggctta tgcccagttc cttgccggta tggcgttcaa taatgcttct
      901 ctgggttatg ttcatgcgat ggcgcaccag ctgggcggtt tctacaacct gccacacggt
      961 gtatgtaacg ccgttctgct gccgcatgtt caggtattca acagcaaagt cgccgctgca
     1021 cgtctgcgtg actgcgccgc tgcaatgggc gtgaatgtga caggtaaaaa cgacgcggaa
     1081 ggtgctgaag cctgcattaa cgccatccgt gaactggcga agaaagtgga tatcccggca
     1141 ggcctacgcg acctgaacgt gaaagaagaa gatttcgcgg tactggcgac taatgccctg
     1201 aaagatgcct gtggctttac taacccgatc caggcaactc acgaagaaat tgtggcgatt
     1261 tatcgcgccg caatgtaatc atcatttcca caacggctgg caaattgtca gccatttttt
     1321 caactatctc tgtaatcctt gcccgtaaat tcgtgatagc tgtcgtaaag ctgttaccga
     1381 ctggcgaaga tttcgccagt cacgtctacc cttgttatac ctcacaccgc aaggagacga
     1441 tcatgaccaa taatccccct tcagcacaga ttaagcccgg cgagtatggt ttccccctca
     1501 agttaaaaaa ccgctatgac aactttattg gcggcgaatg ggtagcccct gccgatggtg
     1561 agtattacca gaacctgacg ccggtgaccg ggcagctgct gtgcgaagtg gcgtcttcgg
     1621 gcaaacgaga catcgatctg gcgctggatg ccgcgcataa agtgaaagat aaatgggcat
     1681 atacctcggt gcaggatcgc gcggcaattc tgtttaagat tgccgatcga atggaacaaa
     1741 acctcgagct gttagcgaca gctgaaacct gggataacgg caaacccatt cgcgaaacca
     1801 gtgctgccga cgtgccgctg gcgattgacc atttccgcta tttcgcctcg tgtattcggg
     1861 cgcaggaagg tgggatcagt gaagttgata gcgaaaccgt ggcctatcat ttccatgaac
     1921 cgttaggcgt ggtggggcag attatcccgt ggaacttccc gctgctgatg gcgagctgga
     1981 aaatggctcc cgcgctggcg gcgggcaact gtgtggtgct gaaacccgca cgtcttaccc
     2041 cgctttctgt actgctgcta atggaaattg tcggtgattt actgccgccg ggcgtggtga
     2101 acgtggtcaa tggcgcaggt ggggaaattg gcgaatatct ggcgacctcg aaacgcatcg
     2161 ccaaagtggc gtttaccggc tcaacggaag tgggccaaca aattatgcaa tacgcaacgc
     2221 aaaacattat tccggtgacg ctggagctgg gcggtaagtc gccaaatatc ttctttgctg
     2281 atgtgatgga tgaagaagat gcctttttcg ataaagcgct ggaaggcttt gcactgtttg
     2341 cctttaacca gggcgaagtt tgcacctgtc cgagtcgtgc cttagtgcag gagtcaatct
     2401 acgaacgctt tatggaacgc gccatccgcc gtgtcgaaag cattcgcagc ggtaacccac
     2461 tcgacagcgt gacgcaaatg ggggcgcagg tttctcacgg gcaactggaa accatcctca
     2521 actacattga tatcggtaaa aaagagggcg cagacgtgct caccggcggg cggcgcaagc
     2581 tgctggaagg cgaactgaaa gacggctact accttgaacc gacgattctg tttggtcaga
     2641 acaatatgcg ggtgttccag gaagagattt ttggcccggt gctggcggtg accaccttca
     2701 aaacgatgga agaagcgctg gagctggcga acgatacgca atatggcctg ggcgcgggcg
     2761 tctggagccg caacggtaat cttgcctata agatggggcg cggcatacag gcagggcgcg
     2821 tgtggaccaa ctgttatcac gcttacccgg cacatgcggc gtttggtggc tacaaacaat
     2881 caggcattgg acgcgaaacc cacaagatga tgcttgagca ttaccagcaa accaagtgcc
     2941 tgctggtgag ctactcggat aaaccgttgg ggctgttctg atgtaagaag ctggtcgcat
     3001 cgggtattaa ttgcctgatg cgacgcttac gcgtcttatc atgcctacgg ggacctgacc
     3061 gtaggccgga taaggcgtac acgccgcatc cggcataaac aaagcgcacg ttgttacaat
     3121 ttacgtttta aacctacgtg cgcaataacc cagactacgc caacagaagt gcgtttctgg
     3181 ggcgtgtttt gccaggagtt taatctcgcg tttcgatggt gagcgggcaa accagaccgt
     3241 aaatcatcat tggtattccg aggacaatca gcaataaacg gtagattatt gaaggtttaa
     3301 tacgcttaat ctgcattgtg gatgttttac ggtttttaat cataaatttt gccaatagtt
     3361 ccattatttc aatatgataa gcctcgacct gttaaaaagc caggcagcac acttcaggaa
     3421 g
//
LOCUS       DAZFKF010000235         3371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFKF010000235 DAZFKF010000000
VERSION     DAZFKF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.237"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..180
                     /locus_tag="ACHKA1_005318"
     CDS             <1..180
                     /locus_tag="ACHKA1_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO5682830.1"
                     /translation="HHVSRDTVHTHIAWLAEQGLVRKRVLINGYFIAELTGRGQDVAE
                     GRASVPGVKKPRARG"
     gene            183..764
                     /locus_tag="ACHKA1_005319"
     CDS             183..764
                     /locus_tag="ACHKA1_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFO5682831.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANALTDELRGVDGMSEQLESRIKGILLGKV"
     gene            764..2428
                     /locus_tag="ACHKA1_005320"
     CDS             764..2428
                     /locus_tag="ACHKA1_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682832.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAANARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            2428..>3371
                     /locus_tag="ACHKA1_005321"
     CDS             2428..>3371
                     /locus_tag="ACHKA1_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFO5682833.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSTALDIPQIASRYVEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGAR"
BASE COUNT          739 a          907 c         1033 g          692 t
ORIGIN      
        1 caccacgtgt cccgcgacac cgttcacacc cacattgcct ggcttgccga acagggactg
       61 gtgcgcaaac gcgttctgat taacggttat ttcatcgcag agctgaccgg ccgggggcag
      121 gatgtggcgg aaggccgcgc cagtgttccg ggtgtgaaga aaccacgcgc aagggggtaa
      181 cgatggacaa gccgacgcgt ggccgcgtgc gcaaggtgga tttgctcccc gacagcatcc
      241 gtaagccgct gctggaaatg ctgcgtgaaa aacgtctgac gcaggtccag atccgcgagg
      301 aaatcaaccg tctgatccgc gaggcaggcc tgccggaaga gcagcagctt tcaccggcgg
      361 ccatcagccg ggaagcctcc cgtaatgagc tgattgcccg caatctgcgt gatttgcgcg
      421 agcagacaaa agccatgatg gctgaactgg gggataagcc gaccggtgaa actacggcgc
      481 tgattctgga gatgtcccgt gccctgatgt accgccgtct gcgtgccgcc accgaatccc
      541 tgaacagcga cagcgacgtg gatatgcgcc tcatcaagga catcctcctg tcggcacagc
      601 gtgcggaaag tgccgccgag cgcagcatta agcgtgaaaa agaaatccgg gccgcatttg
      661 ctgaagagat ggcgaacgcg ctcaccgacg aactgcgcgg cgtggacggg atgagtgaac
      721 agcttgagtc ccgtattaag gggattctgt tgggtaaagt ctgatgaata acgccgaaga
      781 actctccccc ttactgacga ataccgtctc cacccgcaaa attgatctgg ccggtgaaaa
      841 agccctgctg ggcgtggatg tgccggacag ccttgatttg cccggtgata tgccggtatt
      901 tctggattat caggcccgct ggtttgagga tgaaagcgag gtctgcatcg cggaaaaatc
      961 ccgccgtacc gggcttacct gggcggaagc cgggcgtaac gtcattaccg ctgcaaaacc
     1021 gaagcggcgc ggtggccgca atgtgtttta tgtggggtca aagcaggaga tggcgctgga
     1081 atatatttct gcctgcgccc tgttctcccg tgcctttaac cagctggcag atgctgatgt
     1141 gtatgagcag accttctggg atcgggataa aaaagaagaa attctgacct acatgatccg
     1201 ctttccgaac agtggattca aaattcaggc actgtcttcc cgtccgtcaa acctgcgcgg
     1261 ccttcaggga gatgtggtga ttgatgaagc cgcgttccac gagtcactgg atgagcttct
     1321 caaggcggca atggcgctta ccatgtgggg cgcgcgcgtg cgtattatct ccacgcataa
     1381 cggcgtcgat aatctgttta atcagtacat tcaggaagcc cgcgagggac gcaaggatta
     1441 cagcgtccac cgcataaccc tggatgatgc cattgcggac gggctgtacc gtcgtatctg
     1501 ttatgtcacc ggccgcgaat ggtcaccgga aagcgagcag aagtggcgtg atgatctcta
     1561 caaaaacgcc ccgacccgtg aggatgccga cgaggaatac ggctgtatcc cgaaaaaatc
     1621 cggcggtgcc tatattcctc acgcacttat tgaaatggcg atgatccgcg acatcccgat
     1681 tctgacgttt gaagccccgg acgacttcat cagccgtgcc gcatggctgc gtgaatcgga
     1741 agttttaacc tggtgtgaag aacatttaaa accgctttta gaggcgttaa atccccgttc
     1801 ccgcttcagc ttcggggaag actttgcccg caccggtgac ctctcctgct ttgtgctgct
     1861 ggaaatcacc gaatccctgg caaaacgtga ggtgtttcgc gtggagctgc gcaacttgcc
     1921 gtatgcccag caggagcagg tgatgatgta catcctgacc cgcgttccgg ccctggttgg
     1981 tgcagcgttt gacgccaccg gtaacggcgg ctatctggca gaagccgcgc tgctggcctt
     2041 tggcccggac atcatcgact gcgtgatgtt gtcgccgaag tggtacggcg agtggatgcc
     2101 aaaactgaaa gccgagtttg aagatcagaa tatcctcgtc gcccgccatc agaccacgct
     2161 tgatgatttg cgccatgtga aggtggtgaa tggtatcccg caaatcgaca aggggcgcac
     2221 gaaggaccag aacgcaacgg ctgcgaacgc ccgccgccat ggtgattttg ccgtggcact
     2281 ctgtatggca aaccgggcgt catacatgga gggtttcatc ctggatgaat cagcctgtca
     2341 ggcgctgccg gagcggtcgc gggcaatgga gggcggttat cgtgatgacg atgaggcata
     2401 tcatgaattt gatcgggggt gctggtagtg ggacgcataa ttgatcttga cggaaaacct
     2461 ttctcctttg acccggagat gcaaagtacc gcgctggata ttccgcagat tgccagccgt
     2521 tatgttgagc atcccgcctc gggtatcacc ccgaaccggg cggcgcagtg ccttcgcggg
     2581 gctgaacgtg gcgatctgat tgcccagtcc gatctggcgg ctgacattga agaaaaggac
     2641 acccaccttt ttgcggagct gggcaagcga cgtcttgcca ttcagggggt gccatggagc
     2701 attgagccgc caccgaacgc cagcgcgaat gagaaaaagg acgcggaaat gctcgacgaa
     2761 tatctgcatt ccgcagactg gtttgatgcc atgctgtttg acgccacgga tgccatcctg
     2821 aagggctatt cctgcatgga aattgagcac gggatgctcg gtaaaatgca catcatccgc
     2881 gccatccgct ggcgtgacag cgggcatttc tgccttaacc cggatgattt gagcgaactg
     2941 cgactgcgtg acggcagcca tgccggggtg gcgtttcagc cctttggctg gatagtgcat
     3001 cagtcgcgtt cacgcaccgg ctacggtggt gcgacggggc ttgtcagaac gcttatctgg
     3061 ccgttcattt tcaaaaacta ttccgtgcgc gatctggcgg aatttctgga ggtgtacggc
     3121 ctgccgatga aggtcgggaa atacccgtcc ggggcaacac cggagcagaa aagtgccctg
     3181 atgcgggcgg tgatggatat cgggcgacgt acaggcggga tcatcccggc cgggatgtcg
     3241 ctggagtttc aggcggcagc gaacggtcag gccgatccgt ttgaaaccat gatttcgtgg
     3301 ggggagcgtt ccatctccaa agcaatactc ggcggcacgc tgaccacgga agccggagac
     3361 aaaggcgcgc g
//
LOCUS       DAZFKF010000236         3371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFKF010000236 DAZFKF010000000
VERSION     DAZFKF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.238"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..180
                     /locus_tag="ACHKA1_005322"
     CDS             <1..180
                     /locus_tag="ACHKA1_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO5682834.1"
                     /translation="HRVSRDTVHTHIAWLAEQGLVRKRVLINGYFIAELTGRGQDVAE
                     GRASVPGVKKPRARG"
     gene            183..764
                     /locus_tag="ACHKA1_005323"
     CDS             183..764
                     /locus_tag="ACHKA1_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFO5682835.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKA"
     gene            764..2428
                     /locus_tag="ACHKA1_005324"
     CDS             764..2428
                     /locus_tag="ACHKA1_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682836.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMLRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            2428..>3371
                     /locus_tag="ACHKA1_005325"
     CDS             2428..>3371
                     /locus_tag="ACHKA1_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFO5682837.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAALDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVLWSIEPPPNANANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGTTGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGAR"
BASE COUNT          730 a          914 c         1031 g          696 t
ORIGIN      
        1 caccgcgtgt cccgcgacac cgttcacacc cacattgcct ggcttgccga gcagggactg
       61 gtgcgcaaac gcgttctgat taacggttat ttcatcgcag agctgaccgg ccgggggcag
      121 gatgtggcgg aaggccgcgc cagtgttccg ggtgtgaaga aaccacgcgc aagggggtaa
      181 cgatggacaa gccgacgcgt ggtcgtgtac gcaaggtgga tttgctcccc gacagcatcc
      241 gtaagccgct gctggaaatg ctgcgtgaaa aacgtctgac gcaggtccag atccgcgagg
      301 aaatcaaccg tctgatccgc gaggcaggcc tgccggaaga gcagcagctt tcaccggcgg
      361 ccatcagccg ggaagcctcc cgtaatgagc tgattgcccg taacctgcgt gatttacgcg
      421 agcagacaaa agccatgatg gccgaactgg gagacaagcc aaccggtgaa accacagcgc
      481 tgattctgga gatgtcccgc gccctgatgt accgccgtct gcgtgccgcg accgaatccc
      541 tgaacagtga cagcgacgtg gatatgcgtc ttattaagga catcctcctg tcagcacagc
      601 gtgcggaaag tgccgccgag cgcagcatta agcgtgaaaa agaaatccgg gccgcatttg
      661 ctgaagagat ggcaaacgcc gtcaccgacg agctgcgcgg cgtggacggg atgagtgaac
      721 agcttgagtc ccgtattaag gggattctgt tgggtaaagc ctgatgaata acgccgaaga
      781 actctccccc ttactgacga ataccgtctc cacccgcaaa attgatctgg ccggtgaaaa
      841 agccctgctg ggcgtggatg tgccggacag ccttgatttg cccggtgata tgccggtatt
      901 tctggattat caggcccgct ggtttgagga tgaaagcgag gtctgcatcg cggaaaaatc
      961 ccgccgcacc gggcttacct gggcggaagc cgggcgtaac gtcattaccg ccgcaaagcc
     1021 taagcggcgt ggtggccgca atgtgtttta tgtggggtca aagcaggaga tggcgctgga
     1081 atatatttct gcctgcgcac tgttctcccg tgcctttaac cagctggcag atgctgatgt
     1141 gtatgagcag accttctggg atcgggataa aaaagaagaa attctgacct acatgatccg
     1201 ctttccgaac agtggattca aaattcaggc actgtcttcc cgtccgtcaa acctgcgcgg
     1261 ccttcagggg gatgtggtga ttgatgaagc cgcgttccac gagtcactgg atgagcttct
     1321 caaggcggca atggcgctca ccatgtgggg cgcgcgcgtg cgtattatct ccacgcataa
     1381 cggcgtcgat aatctgttta atcagtacat tcaggaagcc cgcgaggggc gcaaggatta
     1441 cagcgtccac cgcattaccc tggatgatgc cattgcggac gggctgtacc gtcgtatctg
     1501 ttatgtcacc ggccgcgaat ggtcaccgga aagcgagcag aagtggcgtg atgatctcta
     1561 caaaaacgcc ccgacccgtg aggatgccga cgaggaatac ggctgtatcc cgaaaaaatc
     1621 cggcggtgcc tatattcctc acgcgcttat tgaaatggcg atgctccgcg acatcccgat
     1681 tctgacgttt gaagccccgg acgacttcat cagccgtgcc gcatggctgc gtgaatcgga
     1741 ggttttaacc tggtgtgaag aacatttaaa accgctttta gaggcgttaa atccccgttc
     1801 ccgcttcagc ttcggggaag actttgcccg caccggtgac ctctcctgct ttgtgctgct
     1861 ggaaatcact gaatccctgg caaaacgcga ggtgtttcgc gtggagctgc gcaacctgcc
     1921 gtatgcccag caggagcagg tgatgatgta catcctgacc cgcgttccgg cgctggtcgg
     1981 tgcggcgttc gacgccaccg gtaacggcgg ctatctggcc gaagccgcgc tgctggcctt
     2041 tggcccggac atcatcgact gcgtgatgtt atcgccgaag tggtacggcg agtggatgcc
     2101 aaaactgaag gccgagtttg aggatcagaa tatcctcgtc gcccgccatc agaccacgct
     2161 tgatgatttg cgccatgtga aggtggtgaa cggtatcccg caaattgaca aggggcgtac
     2221 gaaggaccag aacgcaacgg ctgtgaacgc ccgccgccat ggcgattttg ccgtggcgct
     2281 ctgtatggca aaccgggcgt catacatgga gggcttcatc ctggatgaat cagcctgtca
     2341 ggcgctgccg gagcggtcgc gggccatgga gggcggttat cgtgatgacg atgaggcata
     2401 tcatgaattt gatcgggggt gctggtagtg ggacgtataa ttgatcttga cggaaaacct
     2461 ttctcctttg acccggagat gcagagcgcc gcgctggata ttccgcagat tgccagccgt
     2521 tatattgaac atccggcctc gggtatcacc ccgaaccggg cggcacagtg ccttcgcggg
     2581 gctgaacgtg gcgatctgat tgcccagtcc gatctggccg ctgacattga agaaaaggat
     2641 acccaccttt ttgcagagct gggcaagcga cgccttgcca ttcagggggt gctctggagt
     2701 attgagccgc caccgaacgc caacgcgaat gagaaaaagg acgcggaaat gctcgacgaa
     2761 tatcttcatt ccgccgactg gtttgatgcc atgctgtttg atgccaccga tgccatcctg
     2821 aagggctatt cctgtatgga gattgagcac gggatgctcg gtaaaatgca catcatccgc
     2881 gccatccgct ggcgtgacag cgggcatttc tgccttaacc cggatgattt gagcgaactg
     2941 cggctgcgtg acggcagcca tgccggggtg gcgtttcagc cctttggctg gatagtgcat
     3001 cagtcacgtt cacgcaccgg ttacggcggc acgacggggc ttgtcagaac gcttatctgg
     3061 ccgttcattt tcaaaaacta ttccgtgcgc gatctggctg aatttctgga ggtgtacggc
     3121 ctgccgatga aggtcggtaa atacccgtcc ggggcaacgc cggagcagaa aagcgccctg
     3181 atgcgggcgg tgatggatat cgggcgacgt acaggcggga tcatcccggc cgggatgtcg
     3241 ctggagtttc aggcagccgc gaacggtcag gccgatccgt ttgaaaccat gatttcgtgg
     3301 ggggagcgtt ccatctccaa agctatcctc ggcggcacgc tgaccacgga agccggagac
     3361 aagggcgcac g
//
LOCUS       DAZFKF010000237         3317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFKF010000237 DAZFKF010000000
VERSION     DAZFKF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.239"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..694
                     /locus_tag="ACHKA1_005326"
     CDS             <1..694
                     /locus_tag="ACHKA1_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO5682838.1"
                     /translation="PKNYHFKSKLNYYPITQAAFLGETEVVSNLIKLGVDLDARGDLG
                     RTALHEAVSNGYFDIVKLLVESGADLTIKDEFGKTALELAEVHQNYKIVEWLSHYRDH
                     NPIPDFSVLINIYGERYFGGEIIDTDARTELGEGVLHIAVRKRRQLDVRCFIQHGADI
                     NAKANNGFDFTPLHYSIGIGDFDMMKLLLKLGADIYLESEMGYSPMDLAILMGDKRII
                     FYLYGYISHVSE"
     gene            900..2666
                     /locus_tag="ACHKA1_005327"
                     /pseudo
     CDS             900..2666
                     /locus_tag="ACHKA1_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521871.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            2666..3043
                     /locus_tag="ACHKA1_005328"
     CDS             2666..3043
                     /locus_tag="ACHKA1_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682839.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DVSKFLGDKSKSHALVWKNFGQE"
BASE COUNT          924 a          608 c          960 g          825 t
ORIGIN      
        1 gccgaaaaat taccacttta agtcaaaatt aaattattat cctataactc aggctgcatt
       61 tttaggtgag actgaggttg taagtaattt aattaaattg ggtgtggatc tggatgctcg
      121 tggggatttg gggcgtaccg cacttcatga ggccgtaagt aatgggtatt ttgatattgt
      181 taagctttta gtagaatcag gagccgatct tactataaag gatgaatttg ggaaaacggc
      241 attagaattg gctgaggtac accagaatta taaaatagtt gaatggctat cacattatag
      301 agatcataat cccatacccg atttttctgt acttattaat atctacggtg agcgatattt
      361 tggtggagag attatagata cagatgcaag gacagaactt ggcgaggggg ttcttcatat
      421 agctgttagg aaaagaagac aattagatgt cagatgtttt attcaacatg gtgccgatat
      481 aaatgctaag gcaaataatg gctttgattt cactcctttg cattattcga tagggatagg
      541 ggattttgat atgatgaagc ttttgttgaa attaggtgct gatatttatt tggagagtga
      601 gatgggatac tcccctatgg atttggctat tttgatgggg gataagagaa ttatttttta
      661 tttatatggt tatatttctc atgtttctga ataagcctgg gtgtaatgca atcaaaagat
      721 tgtaacgaat gaaataatct gtaagtgagg tggcgttaat taggacgata atcgcatgaa
      781 aaatcacccc gttaaaattt tttatgaatc attaactagt attgttgctc cttgtatgac
      841 aaaaccacag catgtgcagg aaatatgcac atgttgtgga cgccctgcaa gggaatttcc
      901 cgtatgcaac agacccggcg ggaaaccggc tgccggaccc ggaactgcat ccggacagca
      961 cgctcacggc gtggccggat aaccgcatcg cggaagatgc gcactatgtc taccgctacg
     1021 atgaatacgg caggctggcg gagaagacgg accgtatccc ggaaggggtt atccggatgc
     1081 acgacgagcg cacccaccac tatcactacg acagccagca ccgcctggtg ttctacacgc
     1141 ggatacagca cggcgaacca caggtggaga gccggtacct ctatgacccg ctgggccgcc
     1201 ggacgggaaa acgggtgtgg cggcgggagc gtgacctgac ggggtggatg tcgctgtcgc
     1261 gtaaaccgga ggagacgtgg tacgggtggg acggcgacag gctgacgacg gtacagaccg
     1321 acaccacgcg tatccagacg gtataccagc cggggagctt cacaccactc atcagaatcg
     1381 aaacagagaa ttgcgaacag gcgaaggcgc ggcaccgtag cctggcggag gtgttgcagg
     1441 aggacacggg ggtgacgcta ccggcggagc tggcggtgat gctgggaagg ctggagcggg
     1501 aactgcgcgc gggagcggtg agtgcggaga gtgaggcatg gcttgcgcag tgcgggctga
     1561 cggcggagca gatggccgca cagctggagg cggaatacat cccggagagg aaacttcatc
     1621 tttaccactg cgaccaccgg ggactgccgc tggcgctcat cagcccggaa ggggaaacgg
     1681 cgtggcaggg ggagtatgac gagtggggaa acctgctggg cgaaaccagc gcgcagcacc
     1741 ttcaacagtc actccgtctg ccggggcagc agtatgatga ggagtcgggg ctgtactata
     1801 accggaaccg gtactacgat ccgttgcagg gacgctatat cacccaggat cccattgggc
     1861 tgaagggggg atggaacctg tatgcgtatc cgctgaaccc ggtgcagcgg ggggatcctc
     1921 ttgggttgtc tggtgagaca ctggttcttg gttctgccac aacagtaaca ggaacagaat
     1981 ttgggggggc gttactttcg ggtggggcgg cgacttttga tgcagtcacg gcggctatgg
     2041 cgtctgctgc ccctgctctt ggagcaatgg ctgtggtcgc tgggccaatg ggatatgacc
     2101 ttctcgctaa tgatgggcgt gccgggaagg ctctgagcga tgggcagtta ataaattctc
     2161 aggctgaaat gtgttactgg accagtagtt gtcctttaga tagcccacat gagaatgata
     2221 attcgtcagg gagtcagccg aatgttggga aggatttgac ggatgaagag aagaactgcc
     2281 tggccggcgg cagtacggga agcccgggag gatggggacc agaagatgag aatagcgcaa
     2341 gggatcgtat gcagcatgaa cggtataaag atgaattgcg acagaaaatg ggaaaacccg
     2401 acgttacaga ttcaaatttg aaaaatatta tagacgatct ttatcggcca aatgcaaaag
     2461 taggaagtgg aagcacagca gatgctgttc gttatgaatt ggccacaggt gaaaaagttg
     2521 gtggtagagg acatgtattg aaagcacagg attatacaag ggctttacag gattggttgg
     2581 ataagaatcc aacagcatct ccaggggatc gagcagcagc agaaaacgtt cttcaggatt
     2641 tacagaatgc tttgggaggc aagtaatgaa ccaatatatt gctagttata tagaaaaaat
     2701 ggcatcattt tccactgttt tgtctgagac agtctcatta gctaatgaat actggcatcc
     2761 agaaacgcca ccggtggtta tgttgttctc tcaggttgga aaaacgattg taaaaaattt
     2821 ttatgaaatg ggggatattg aaagagaaaa aatatttaaa catgtcgaag atggtatggt
     2881 ctcgcttgat gatgaattgg ccacagttgt agctacaggg cttatagagg caatagtcac
     2941 tgcgaccgat agcaattctt tactttggca agatgtaagt aaatttctgg gtgacaaatc
     3001 aaagtctcat gcattagtgt ggaaaaattt tggtcaggag tgatattatt caagaagtat
     3061 taagctcgct attgagatct taatgcttct tgagattaaa ttaaagttag gacgaagtaa
     3121 ttgcaggtgg aaataaatcc atggtgacgt gtggcaatgg ctattagatg ttatggtgtg
     3181 atgggttttg gtgatttaag agctaagtta gattgcctta tgaaaaagta tggttatata
     3241 tttaatgatg tgtatcatat atttaaataa aaaacggttc tctcttgtca agatagtaat
     3301 aggatagatt attgtgt
//
LOCUS       DAZFKF010000238         3234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFKF010000238 DAZFKF010000000
VERSION     DAZFKF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.240"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..734)
                     /gene="sctW"
                     /locus_tag="ACHKA1_005329"
     CDS             complement(<1..734)
                     /gene="sctW"
                     /locus_tag="ACHKA1_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA];
                     GO_process: GO:0050709 - negative regulation of protein
                     secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     SctW"
                     /protein_id="HFO5682840.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMGDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRMLVT
                     KFIKETLFSDINALDASCSSLEFGMFL"
     gene            complement(739..2442)
                     /gene="sctC"
                     /locus_tag="ACHKA1_005330"
     CDS             complement(739..2442)
                     /gene="sctC"
                     /locus_tag="ACHKA1_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HFO5682841.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGGGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVSRMPPFPRAEEDDDLQVEKIISTAGAPETDDIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKI
                     GSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRT
                     FYTKLVGERTAELDEVTYGTMISVLPRFAARNQIELLLNIEDGNEINSDKTNVDDLPQ
                     VGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNI
                     VRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVG
                     G"
     gene            complement(2439..3188)
                     /locus_tag="ACHKA1_005331"
     CDS             complement(2439..3188)
                     /locus_tag="ACHKA1_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5682842.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
BASE COUNT          911 a          717 c          531 g         1075 t
ORIGIN      
        1 agaaacatac caaattccaa tgatgaacaa ctcgcatcaa gtgcatttat atctgagaaa
       61 agcgtctctt tgataaattt tgtcaccaac attctatttt gatagccata actaccaatc
      121 cattcgacat aagtatctac agcatcgtct tctgccatta aaaattgccg ataactcgcc
      181 cttagtaatt ttggatttaa tgacagcgcc tggccaaata actttgcttt taatgccgag
      241 ttaatgccgc tattaatgaa ttttttagta gtctcttgat taacttttct aagtaatgct
      301 tcagcgttta gccggacatt ttctgatatt tgttttttac gtaaaattgc acgtaacaca
      361 actaccagat cgctttcatc tgggaataat tttttagcaa agctatacac ttcatagtca
      421 tgggtagttg cagccttaag cgcctccata actttgtcgg ttttttctat tggctggtct
      481 tccaggacat attcttctcc gtccagaaaa ttggccagtt gttcttcgta gatttttttc
      541 ttcataaact gcgttaacgc tgctgacatc tcgtcagtag cttgtaagaa ccgagcttct
      601 ttacctgcgg gatcatcccc cattttaggc atttcagctt caatatcttc attatcttct
      661 gtgataatgt cctctaagcg agaaacttcc ctggctcttt ctaatacacc gacatgctct
      721 acatgaatcg ccatttcact atcctccgac gacttcacga ttcaggtaag tttttatcca
      781 tttctgcagt aattcatcgt taatttcttt attttttgcc atttgctgtg taatcgctct
      841 ggcatcaacc gcagcctcat tcgcattatt catcatgcgc tcatcgatct ctcttggctc
      901 aataagaaag acacgaacga tgttattcgc atttgttcct tcataaccaa ataatttacc
      961 aataaatgga atgctgccta atattgggat cttgcgactt tcataggtat tcgtatcgcg
     1021 tgtatatccc ccaatcaaaa gacttttccc ttggggcacg cgtgcaatag tacttataag
     1081 tgttctacca acctgaggca gatcatcaac attggtcttg tcggaattga tttcatttcc
     1141 atcttcaata ttcaagagta attcaatttg atttcgcgca gcaaatcgtg gtagcacgct
     1201 aatcatcgta ccataggtta cttcatccaa ttccgcagta cgctcgccga ctaatttagt
     1261 atagaaagtg cggttattat caaatatagc aggaatattc tcctgtgtta aaacgacggg
     1321 acgagcaaca acggcggctc ttcttttttg tgcaagtgcc tgtattgttg caataaactg
     1381 tgtcccatca agggtactaa ttgaacctga attattaaat gatgcactta agctggatcc
     1441 aatttttatc gtgcccgacc agtcagcacc tagctgttct agatcagtct tatcaatatc
     1501 tattatccat aaagataatt caatgtgtcg ctttggaata tcaagcgtag ctactaactt
     1561 ctcgataaaa tcaacctgag atactgttcc tttaacaagt aaactgttgg tatcaggata
     1621 tgcaataatt tgtatatcgt cagtctctgg cgctcctgct gtcgatatta ttttctctac
     1681 ttgtaggtca tcatcttctt cagccctagg aaaaggaggc atacgtgata cgttttttag
     1741 ttgttgacga cttgaaattt cagaaagtac gttcacccct gtgctttgct taatgttatt
     1801 atttaacaac gtcgatagta cttttgccat gccaggaata actatctttt cgccacgtaa
     1861 ctcataagtc cggtcattaa cgaatgtgtt gaccagatga attattccta ctttatttcg
     1921 accgatctca atgccatcgc tgttttgctc cattaatttc gcagcattaa cgaccaggtc
     1981 aacataaacc ggtggccccg atacatagaa agtaccattg ccgccccctt taatttcata
     2041 acgagagtta tacagacccg atttttttaa gaaattatta aactcattcg tcgatacctt
     2101 tctcagatta atcaatgcat tacgcatctc tgatgagtca tagatataaa tagctttgcc
     2161 atcatcatac caaataagcc ccatctgtgc cgacaggcgt tcaatcaagg catagggttc
     2221 tgttaaatca aaatttccgc tgatctgttt cttcatggca agcttactaa ctattgtagg
     2281 cttacccgca tatgatgaaa ccgtttcaaa aaaactacgt agattttctt tatttgctac
     2341 gtatccattc gctgctgcgg catatgcacc aggagtcaat aatccattaa aaatgcataa
     2401 gactgaaatt aatataatag taatgcgtaa ttttattttc ataacttttt acatagttct
     2461 ctcggtgttt ttcctaaccg gctttttact tcggctgaaa agtgtgatga ggacgagtaa
     2521 ccatatttat gcgcaatagt tgtcatatca ctgtttcctt caatgatttc caacacggct
     2581 ctggcaactc tccagccaca taattccgtt ttaactttac cacccaatgc ataactacat
     2641 aaacggcgaa aatgtgaata cgatacgccg taaagctcac caagatcata gagtgaagtc
     2701 tgtatgagcg attgcgataa taaataacga accagtttat agcattctgt ttttcttaga
     2761 tgctgaaaca ggatttcatt aatattatcg ctgctcagat aagtggctaa aaaccaggct
     2821 tcacttgcaa ctttactgaa tagcacaact tctgatgcaa agggcaaggc atcagcaaac
     2881 aattcatgat gttccctttc cacactcccc gttgattcat ctataaaagc taataattta
     2941 ctcaggatat caatgcgaag tggatgataa attactttcc ctgcagttac ctcaatttgg
     3001 cttcggtcaa tgagcagtaa tatatcttcc actaccatta atttttgatg gttattacca
     3061 atcgtaataa caagggttac atccttgttt tcatttcccc gtaagaacca catttcaggg
     3121 tccagtaggg ttaacttctg tccttgaagc aaaacattca ttggaagtaa cagcccttct
     3181 tcaatcatgt ttcatctcct tattccatat tctcattctc cataaacttg ctat
//
LOCUS       DAZFKF010000239         3227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFKF010000239 DAZFKF010000000
VERSION     DAZFKF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.241"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(87..614)
                     /gene="mobB"
                     /locus_tag="ACHKA1_005332"
     CDS             complement(87..614)
                     /gene="mobB"
                     /locus_tag="ACHKA1_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO5682843.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
     gene            complement(596..1180)
                     /gene="mobA"
                     /locus_tag="ACHKA1_005333"
     CDS             complement(596..1180)
                     /gene="mobA"
                     /locus_tag="ACHKA1_005333"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO5682844.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1250..1519
                     /locus_tag="ACHKA1_005334"
     CDS             1250..1519
                     /locus_tag="ACHKA1_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO5682845.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1596..2582
                     /gene="srkA"
                     /locus_tag="ACHKA1_005335"
     CDS             1596..2582
                     /gene="srkA"
                     /locus_tag="ACHKA1_005335"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO5682846.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFNHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2599..3225
                     /gene="dsbA"
                     /locus_tag="ACHKA1_005336"
     CDS             2599..3225
                     /gene="dsbA"
                     /locus_tag="ACHKA1_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO5682847.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          817 a          782 c          790 g          838 t
ORIGIN      
        1 aggccatcct gacggatggc ctttttgcgt tggtgcaaaa aaatgccgga tgcgacgctg
       61 gcgcgtctgc tccagcctac tcttgcttat ccgtcctgat tttgcatcca ctccaccaca
      121 aaatcagcca gcccctcaac atcattaata tccagtaatg cgacatcaag attaagcggc
      181 acatcactgg ctacagcaat aacatgcctg tctatcacta attcttcagg tcgatgtccg
      241 gctccatcgc gaaacagcac aatctttgcg atctcttcat gcttaaaccc ttcgaccaga
      301 atcaaatcca gctttgaggt atccattcga cttgcgagaa actgtagatc cagctcttct
      361 tcgtctggtg tttccgtcat caaggcccat cgttgctggc tggcaacgat ggtttgtgcc
      421 gcgccagcct tgcgcagctc atagctatct ttgcctggct tatcaacatc catatcatgg
      481 tgcgtatgct taatcagccc tggacggatc cctctggcgc ataatgccgg gatcagtttt
      541 ttcaaaagcg tagtttttcc ggtaccactc cacgcggcaa aggcgagtaa cggtatcatc
      601 gtttttcctg ccatcgggca agctcctctg gcgtattcac gttaacaaat gcatctttat
      661 gatcgctgaa atcaaccgca tgaccgccag ccagacgcat aaatgccatt acccggcgtt
      721 ctcctgcttg cagatattct agtaataaag gctcaatagc gcggtttacc agagcaatag
      781 tcgggtgatc gcgttcaccg tcatggaccc acacgacagg cgcatctttg cgctgatgat
      841 taagccgggc ggctaaatca tggggaatgt aaggcgtatc gcacggacaa aacaaaaacc
      901 actcaccagc ttcctgctgc attactgaaa gcatccctgc cagagggcct gggtaatccg
      961 ccagtgaatc ttcaatcact ttcagaccgc ctgcctgata gatttcctga tgacgattag
     1021 cattaaccac gacgtgagag agctgcgtca taagcgcgtc agcgacatgt tgccataatg
     1081 gtttgccgtt taattcaagc aatcctttat ctacgccgcc cattcgtctg gctttaccgc
     1141 ctgccagcac aacgcctgtt atcgtcgtca tcagattcac cgatatcgcc tcttttattg
     1201 tgggattgac cctgctaacg tgtctgtctc aagagtaagg agcatcacta tgaaatgtaa
     1261 acgtctgaat gaagttattg aactcctcca gccagcctgg caaaaagagc cagaccttaa
     1321 cctgctgcaa tttttgcaga aactggcgaa agagtcaggt tttgacggcg aactggcgga
     1381 tttgacggat gacattctga tctatcacct gaaaatgcgt gattccgcga aagatgcggt
     1441 gatcccgggt ttgcagaaag attatgaaga agacttcaaa acggcgctgt tacgcgctcg
     1501 cggcgtaatt aaagagtaaa agcttgtaag cggcgccacc aaaatcatcg tgaaatgata
     1561 tccttcatca ttcgtaatgt tttccggatg atgggatgaa caacagcgct tttactttcc
     1621 agacactaca cccggatacc atcatggatg ctctgtttga gcaagggatc cgggtggatt
     1681 ccggtcttac cccgcttaac agctatgaaa accgtgtcta tcaatttcag gacgaagatc
     1741 gtcgacgttt tgtcgtcaaa ttttatcgcc ctgaacgttg gacagccgat caaatcctcg
     1801 aagaacatca atttgcgttg cagctggtaa atgatgaagt cccggtcgca gcacctgtgg
     1861 cctttaacgg tcagacttta ttgaatcatc agggatttta tttcgctgtt tttccaagcg
     1921 tcggtggtcg ccagttcgaa gctgataata tcgatcagat ggaagcggtt gggcgttatt
     1981 tagggcgtat gcaccagacg gggcgcaaac agctttttaa ccatcgcccg accatcggtc
     2041 tgaatgaata tctcattgag ccacgcaagc tgtttgagga cgctacactg ataccttccg
     2101 ggttgaaagc ggcattcctg aaagcgacag atgagctgat cgccgccgtt acagcacact
     2161 ggcgggaaga tttcaccgtt ctgcggctac atggagactg ccacgccggg aatattctct
     2221 ggcgcgatgg cccaatgttt gttgatctgg atgatgcacg taatggtcca gctattcagg
     2281 atttgtggat gttgctcaat ggcgataaag ccgagcagcg gatgcaactg gaaactatta
     2341 ttgaagctta tgaagaattt agcgagttcg acaccgctga aatcggactg attgaacctt
     2401 tacgcgccat gcgtttggtt tattatcttg cctggctaat gcggcgttgg gctgatcccg
     2461 cgttcccgaa aaatttcccg tggttaaccg gggaagatta ctggctacga cagacggcga
     2521 cttttataga acaggcaaaa gttctacaag aacccccttt gcaattaaca cctatgtatt
     2581 aatcggagag agtagatcat gaaaaagatt tggctggcgc tggctggttt agttttagcg
     2641 tttagcgcat cggcggcgca gtatgaagat ggtaaacagt acactaccct ggaaaaaccg
     2701 gtagctggcg cgccgcaagt gctggagttt ttctctttct tctgcccgca ctgctatcag
     2761 tttgaagaag tactgcatat ttctgataat gtgaagaaaa aactgccgga aggcgtgaag
     2821 atgactaaat accacgtcaa cttcatgggt ggtgacctgg gcaaagatct gactcaggca
     2881 tgggctgtgg cgatggcgct gggcgtggaa gacaaagtga ctgttccgct gtttgaaggc
     2941 gtacagaaaa cccagaccat tcgttctgct tctgatatcc gcgatgtatt tatcaacgca
     3001 ggtattaaag gtgaagagta cgacgcggcg tggaacagct tcgtggtgaa atctctggtc
     3061 gctcagcagg aaaaagctgc agctgacgtg caattgcgtg gtgttccggc gatgtttgtt
     3121 aacggtaaat atcagctgaa tccgcagggt atggatacca gcaatatgga tgtttttgtt
     3181 cagcagtatg ctgatacagt gaaatatctg tccgagaaaa aataatt
//
LOCUS       DAZFKF010000240         3208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFKF010000240 DAZFKF010000000
VERSION     DAZFKF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.242"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            162..1844
                     /gene="ydeN"
                     /locus_tag="ACHKA1_005337"
     CDS             162..1844
                     /gene="ydeN"
                     /locus_tag="ACHKA1_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5682848.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1896..3053
                     /gene="ydeM"
                     /locus_tag="ACHKA1_005338"
     CDS             1896..3053
                     /gene="ydeM"
                     /locus_tag="ACHKA1_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO5682849.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFTKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEVFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCLKCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3019..3141)
                     /locus_tag="ACHKA1_005339"
     CDS             complement(3019..3141)
                     /locus_tag="ACHKA1_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682850.1"
                     /translation="MTTHPTGIYVGCFVKVIRRRLRLELRGRVINYSPFVLQHP"
BASE COUNT          975 a          693 c          647 g          893 t
ORIGIN      
        1 tacttagtag aacagaaagt aatgtcaacg ctttatggac tgttatttcc ctttttttag
       61 ctaaatctgc tatctcttta tgtgactaac ttcacttaca tccacttatt tctcttcgta
      121 aaattacttt ggaattaagt acaataagaa gaggaacatt tatgaagtct gcattaaaga
      181 aaagtgtcgt aagtacctcg atatctttga tactggcatc tggtatggct gcatttgctg
      241 ctcatgcggc agatgatgta aagctgaaag caaccaaaac aaacgttgct ttctcagact
      301 ttacgccgac tgaatacagt accaaaggaa agccaaatat tatcgtactg accatggatg
      361 atcttggtta tggacaactt ccttttgata agggatcttt tgacccaaaa acaatggaaa
      421 atcgtgaagt tgtcgatacc tacaaaatag ggatagataa agccattgaa gctgcgcaaa
      481 aatcaacgcc gacgctcctt tcattaatgg atgaaggcgt acgttttact aacggctatg
      541 tggcgcacgg tgtttccggc ccctcccgcg ccgcaataat gaccggtcga gcgcccgccc
      601 gctttggtgt ctattccaat accgatgctc aggatggtat tccgctaaca gaaactttct
      661 tgcctgaatt attccaaaat catggttatt acactgcagc agtaggtaaa tggcacttgt
      721 caaaaatcag taatgtgccg gtaccggaag acaaacaaac acgtgactat catgacaact
      781 tcaccacatt ttctgcggaa gaatggcaac ctcaaaaccg tggctttgat tactttatgg
      841 gattccacgc tgcaggaacg gcatattaca actccccttc actgttcaaa aatcgtgaac
      901 gcgtccccgc aaaaggttat atcagcgatc agttaaccga tgaggcaatt ggcgttgttg
      961 atcgtgccaa aacacttgac cagcctttta tgctttacct ggcttataat gctccgcacc
     1021 tgccaaatga taatcctgca ccggagcaat atcagaagca atttaatacc ggtagtcaaa
     1081 cggcagataa ctactacgct tccgtttatt ctgttgatca gggtgtaaaa cgcattctcg
     1141 aacaactgaa gaaaaacgga cagtatgaca atactattat tctctttacc tccgataatg
     1201 gtgcggttat cgatggtcct ctgccgctga acggggcgca aaaaggctat aagagtcaga
     1261 cctatcctgg cggtactcac accccaatgt ttatgtggtg gaaaggaaaa cttcaacccg
     1321 gtaattatga caagctgatt tccgcaatgg atttctaccc gacagctctt gatgcagccg
     1381 atatcagcat tccaaaagac cttaagctgg atggcgtttc cttgctgccc tggttgcaag
     1441 ataagaaaca aggcgagcca cataaaaatc tgacctggat aacctcttat tctcactggt
     1501 ttgatgagga aaatattcca ttctgggata actaccacaa atttgtccgt catcagtcag
     1561 acgattaccc gcataacccc aacactgagg acttaagcca attctcttat acggtgagaa
     1621 ataacgatta ttcgcttgtc tatacagtag aaaacaatca gttaggtctc tacaaactga
     1681 cggatctaca gcaaaaagat aaccttgccg ccgccaatcc gcaggtcgtt aaagagatgc
     1741 aaggcgtggt aagagagttt atcgacagca gccagccacc gcttagcgaa gtaaatcagg
     1801 agaagtttaa caatatcaag aaagcactaa gcgaagcgaa ataactaaac cttcatgcgg
     1861 cggaattttc cgccgcctta ttgagcgaga tagcgatgca cgttacagcc aagccctcca
     1921 gttttcaatg taatctcaaa tgtgattact gtttttacct tgaaaaagag tcgcagttta
     1981 ctcatgaaaa atggatggat gacagcactc tgaaagagtt catcaaacaa tatatcgcag
     2041 cgtctggcaa tcaggtctat tttacctggc aaggcggtga acccactctg gctggcctgg
     2101 attttttccg taaagttatt cactatcaac aacgctatgc aggccaaaaa cgtattttta
     2161 atgcattaca aacgaatggc attttattga ataatgaatg gtgtgccttt ctcaaagaac
     2221 atgaatttct ggttggtatc tcgatcgatg gcccccagga gttacatgac cgttacagac
     2281 gcagtaattc aggtaacggt acttttacaa aagtgatagc agccatcgag cgtctgaaat
     2341 catatcaagt agagtttaat acgttaaccg tcattaataa cgttaatgtc cattaccctc
     2401 ttgaggttta tcatttttta aaatctatcg gcagtaaaca tatgcaattt atcgaattgc
     2461 tagaaaccgg gacgccgaat attgatttca gtggtcatag tgagaacaca ttccgtatca
     2521 ttgatttttc tgtgcctccc acggcttatg gcaagtttat gtcaaccatt tttatgcaat
     2581 gggttaaaaa cgatgtgggt gaagttttca tccgtcagtt tgaaagcttt gtcagccgtt
     2641 ttttggggaa tgggcatacc agttgtattt tccaggagtc ctgcaaggat aatctggttg
     2701 ttgaaagtaa tggagacatt tacgaatgcg accattttgt ctatccacag tacaaaattg
     2761 gaaacattaa taaatctgaa ctcaaaacga tgaacagtgt acaactgaca gcgcaaaaaa
     2821 aacggatttc agcgaaatgt ctgaaatgtg catataaacc tatctgcaat ggcggttgtc
     2881 ctaagcatcg tattactaaa gtaaacaatg agactgtttc ttatttttgc gaaggttata
     2941 aaatcctttt ttcaaccatg gtaccttata tgaacgccat ggtagagtta gctaagaaca
     3001 gagtaccgct ttatcacatt atggatgttg caaaacaaat ggagaataat taataacacg
     3061 ccctcttagc tccagtctta gcctacgtct tatgacctta acaaagcacc cgacataaat
     3121 tcctgtcggg tgtgtcgtca tcatcatctt accttgcgaa aatcctggca tacgtggtat
     3181 gcgtcagggc attagcaata cccttgat
//
LOCUS       DAZFKF010000241         3142 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFKF010000241 DAZFKF010000000
VERSION     DAZFKF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.243"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..919)
                     /gene="rfaC"
                     /locus_tag="ACHKA1_005340"
     CDS             complement(<1..919)
                     /gene="rfaC"
                     /locus_tag="ACHKA1_005340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO5682851.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLAD"
     gene            complement(923..1969)
                     /gene="rfaF"
                     /locus_tag="ACHKA1_005341"
     CDS             complement(923..1969)
                     /gene="rfaF"
                     /locus_tag="ACHKA1_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO5682852.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(1979..2911)
                     /gene="rfaD"
                     /locus_tag="ACHKA1_005342"
     CDS             complement(1979..2911)
                     /gene="rfaD"
                     /locus_tag="ACHKA1_005342"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO5682853.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
BASE COUNT          750 a          846 c          767 g          779 t
ORIGIN      
        1 aatctgcaag agacatactt gtagaacgac actctacttg attcttccca tatccgccaa
       61 ttaatcccgg atcggttggt ccataaaccg tgatattggg tctatccagt gccgccgtta
      121 aatggcttaa ccccgtatcc accgacacta caaatttagc cccggccagc acgcgggcaa
      181 cgccttccag actcatcttc ggcaatactt caacataagc aaatccttcc gccagtcgtt
      241 tcgcccgttc ttcctcatgc ggcgcgcccc acggaagttt aatccgtatt cctgaatcag
      301 ccagtaaacc aatcaattct cgccagtgtt cttccggcca gtgtttatca tcacgggtcg
      361 tcgcatgaag aaatacggca tattcgccag catctgtagg caggttcgtc agaaaatgct
      421 gtgcgatagc ataatcgccc tgggtttgcg gtttgctata gcccaaactt ttggcaaata
      481 gttcgcgggt gcgttctacg gcgtgctgct gttttgcaat atgatgctta cgattgtaaa
      541 acaggctggc taaaggttcg cgagcggttt gccagtccat gccatgcttt acgccatgcg
      601 ccagacgcgt caccagcgcc gcgcttttta ccagcccctg agcgtcgata actgcgtcat
      661 agttctctgc ttgtagcgct tcacgaaacg ctttgcgttc cgcttttatg ggggccgaga
      721 accaggcttt acgccagcga cgtattgcca caggaataac tcgctcaacg gcagcgtgcc
      781 aggaaggaat ctgtgcgaac ccttcttcca ccacccagtc aaacttaatc cctgggattg
      841 cctgctgggc atcagtgagt gcgggcaacg tatggagaac atcgcccatc gacgatgttt
      901 taacgatcaa aacccgcatc cgtcaggctt cctcttgtaa caatagcgcg ttgagttctt
      961 ccagtacgcg ctggggagta atgtcgatta agctctggtg ataaccctcc gcagcgtcac
     1021 ctttacgcac tttgtgatag ccggtaatca aacggatcac gcgcgcttta tgggatagcg
     1081 gcggtgtgaa gtccgggcta ctcggaccat acagggcaac cagcggacga ttgagcgccg
     1141 ccgcaacatg catcaggcca gaatcgttag tgacaatggc tttacaggct gcaatcagga
     1201 taaccgcttg atcaagctgt gtttcccccg ccaggttccg acaccatgcc tgctgctcgg
     1261 tattcaaagc ggcaagaatc tcattgcccg cttcatgatc tttcgccgag ccaaacagaa
     1321 ccacctgata accttcatca atcagctgct ttgccagctc cgcatagtgg tagtgtggcc
     1381 agcgttttgc cggaccaaac tccgcacccg ggcaaaaacc aatcatcgga cgttctgatg
     1441 aaagcgaaaa ttgattacag gtatatgatt tttcaccttc gctcacctgc aactgcggcc
     1501 ataacaatgg ctgcggcaga tcttgtgctg tgcgcataat gcctttgtca taggccagcg
     1561 ctatatagcg ttccaccatt agcggccagg cttctttatc gagcacgcgt acatcgttga
     1621 gtaaaccgta gcgcatctcg ccgcgccagc cggtgcgatg aggaataccc gcgaagaaag
     1681 gcactaatgc agatttgaag gagttgggta agacgtaggc gcggtcgtag cgcttttcac
     1741 gcagactatg acccagtttg cggcgttcgc cgatttccag cgctccgtga ccgagaggca
     1801 taggaatagc ttcgttaact tccggcatcc gcgataataa tggacggcac catgccggtg
     1861 ccatcacatc gattatcgcc tggggatagc gcgcctggag cgtgcgatag agactttgcg
     1921 acatcatcat gtcgccaacc caagacgggc cgatcaccag tattttcatg cagagctctt
     1981 atgcgtcgcg attcagccag gccatgtatt ccgttacacc ttcagcaacg gttttgaacg
     2041 gtttgtcgta acccgccgcg cgcagatttg tcagatctgc ctgagtgaac gcctgatagc
     2101 ggcctttcag tttatccggg aacggaatgt attcgatctg gcctttcttg tgataagcca
     2161 gcgtagcatc agctacagcc tggaaggatt ccgcacgacc agtaccgaga ttgaagatgc
     2221 cggaaacgcc attttccagg aaccacagat tcacatcagc cacgtcgccc acatagacga
     2281 agtcgcgttt gaagttctcg ctaccttcaa acagcttcgg tgattcaccg ttgttaagct
     2341 gggtgttgag atggaaagcg acgctcgcca tgctgccttt atggccttca cgcggtccat
     2401 aaacgttgaa ataacggaag ccaacaatct gcgagttcgc ttcaggcagg atttgacgaa
     2461 catattcatc aaacaggaat tttgagtaac cgtagacgtt caacggtttt tcgtactcgc
     2521 gggattcaat aaagtcggag gtgcgtccgc cgtaggtggc cgcggaagat gcatacagga
     2581 acgggatttc gcgctccaga cagtagtgca gcagctcttt ggagtattga tagttgttat
     2641 ccatcatata cttgccatcc cactcggtgg tggaagagca tgcgccttcg tggaaaatcg
     2701 cttcgacatc gccgaactct tcgccagcca taatctggat caggaagtct tccttatcca
     2761 tatagtccgc gatatccaga tccaccaggt tcacaaactt ggtgccgtct ttcaggttgt
     2821 ccaccaccag aatatcggtg atgcctttat cattcagggc cttaacgatg ttgctgccga
     2881 taaagcccgc gccgccggta acgatgatca taactgtaac cttcgaatta tggagtcaga
     2941 gacaatctca gacatgaatg tctgtaatca taccatcaca tccgaatccc ttcagccctt
     3001 tacatagaga tgcaaggatg ctaattgcaa caaactagtt tgggttttag ctagtccttc
     3061 atggcggatg ttggttttgc acgtgaatag tgctttagta tcaatattcc gatgtatggt
     3121 aaataacgga agcatttgaa tg
//
LOCUS       DAZFKF010000242         3096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFKF010000242 DAZFKF010000000
VERSION     DAZFKF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.244"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..248
                     /locus_tag="ACHKA1_005343"
     CDS             <1..248
                     /locus_tag="ACHKA1_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682854.1"
                     /translation="IFYELDGEWYHEICRNAIKRGGQRGEHLRANIDRANVHKFRQFR
                     YIRFLNKRAKKRLNTKLFKVQPYPKPQTVKTGLKESE"
     gene            377..3096
                     /locus_tag="ACHKA1_005344"
                     /pseudo
     CDS             377..3096
                     /locus_tag="ACHKA1_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          807 a          798 c          930 g          561 t
ORIGIN      
        1 caatattcta tgaacttgat ggcgagtggt atcacgagat ttgcaggaat gccattaaac
       61 gaggcggaca acgaggtgaa catctgaggg ctaatattga cagagccaat gttcataagt
      121 tccgacagtt tcgttacatc cggtttctga acaagagggc caaaaaacgc ctcaacacaa
      181 agctattcaa agtccagcct tacccaaaac cacaaacagt taaaactggt ttgaaagaga
      241 gtgaatgagc gcttaaaaac tcattctaaa actcaaaaga aaagcgccga tttagtatca
      301 aataagttgc gtatcggcgt ttttcagcag tatcaaatga aatgcaaatc ggtatcaaaa
      361 aaatcgccgc gctatatggg atgaatacca tcacctgctg gcccgccact cccctgccgg
      421 gcgggtggag aaatttgaat acaacgccgc acatggtcag ttaagctgtt atacggcggc
      481 agacggcgcg gagtggcagt actgctatga tgagcgcggc ctgctcagca acatcaccga
      541 ccctgccggg cagacgtgga cgcagcagtg tgatgaacgc ggcctgccgg tgagtctggt
      601 gtcgccacag ggcgaagaga cccggctggc atacaccgca caggggctgc tgtcgggaat
      661 attccgccag gatgaacggc acctgggcat agagtacgac caccacaacc ggccggaaac
      721 actcaccgac gtgatgggcc gtgaacacca caccgaatac agtggtcacg acctgccggt
      781 gaagatgcgc ggccccggcg gttagtcagt gcggttgcag tggcagcagc accataaact
      841 gagcggcctt gagcgggcag gaaccggcgc agaaggattc cgctatgacc gccacggcaa
      901 cctgctggcg tacacggacg gtaacggcgt tgtctggaca atggaatacg gcccgtttga
      961 tttgccggtg gcgcgaacgg acggtgaagg ccaccgctgg cagtaccgct acgataaaga
     1021 cacgctgcaa ctgacagaag tcattaaccc gcagggcgag tcttatcttt atattctgga
     1081 caactgtggc cgggtgacgg aagaacgtga ctggggcggc gtggtctgtc gttaccgcta
     1141 tgacgccgat ggcctgtgta ccgccagggt taacggcctg gaggaaacta tcctctacag
     1201 ccgggacgcc gcaggccgcc tggcagaaat catcacgccg gaaggcaaaa cgcagtatgc
     1261 gtatgacaaa tccggcaggc tgacgggtat cttcagcccg gacggcacat cacagcgcac
     1321 cggctatgat gaacgcgggc gggtgaatgt caccactcag ggccgacggg ccattgaata
     1381 ccactacccc gacgaacaca ccgtcatccg ctgtatcctg ccaccggaag atgaacgcga
     1441 cagacacccc gatgaatccc tgctgaaaac cacgtaccgt tataacgccg ccggagaact
     1501 gacggaggtc attctgccgg gggatgagac gctgacgttc agccgtgatg aggcgggacg
     1561 tgaagtgctc cggcacagta accggggttt tgcctgtgag cagggctgga atgcagccag
     1621 ccagcttgtc acccagcgcg ccggattttt cccggaggaa accacatggg gcgggctgct
     1681 cccctcactg gtacgggagt accgttacga cagcgcgggc aatgtgtcgg ctgtcaccag
     1741 ccgggaagat tacggacggg aaacacggcg ggagtaccgg ctggaccgga acggtcaggt
     1801 cacggcggtg acagcctcag gcaccgggct gggctatggc gaaggcgatg agtcctatgg
     1861 ctatgacagt tgtggctacc tgaaggcgca gtctgcgggc aggcaccgga taagtgaaga
     1921 gactgaccag tatgccggag gccaccggct gaaacaggcc ggaaacacgc agtatgacta
     1981 tgacgccgca ggccggatgg tcagccgcac aaaacaccgt gacggctacc gcccggaaac
     2041 agagcggttc cggtgggaca gccgggacca gctgaccggg tattgcagcg cacagggtga
     2101 acagtgggaa taccgccacg acgccagcgg cagacgaaca gagaaacgct gtgacaggaa
     2161 gaaaatccgc ttcacgtacc tgtgggatgg cgacagtatt gcggaaatcc gggaataccg
     2221 cgatgatgaa ctgtacagcg tacggcacct ggtgtttaac ggctttgagc tgataagcca
     2281 gcagtgcagc cgggtacggc agccgcaccc gtccgtggcc ccgcagtggg tgacgcgaac
     2341 gaatcatgcg gtgagcgacc tgacgggccg cccgctgatg ctctttaaca gtgaaggtaa
     2401 aaccgtctgg cggccggggc agaccagcct gtgggggctg gcactcagcc tgcccgcaga
     2461 caccggctac ccggacccgc gcggggagcg ggacccggaa gcggaccccg gcctgctgta
     2521 tgcgggacag tggcaggatg cagaatcggg gctgtgctat aaccggttcc ggtactacga
     2581 gccggaaacc gggatgtacc tggtgagtga tccgctgggg ttgcagggag gggagcagac
     2641 ttaccggtat gtgccgaatc cttgtgggta tatcgatcct ttggggctgg ctatatgtca
     2701 gttagcccgc tggacgaaat gggggagtga gcaaagcaac atatctgatg ttttgaactc
     2761 attagggaat agagcactta aatatgctaa tggtgattgg ataaaatcag aggctgcatt
     2821 caataaatac ataaacatga taaataaaag actagaatta acaggtagta aatttagagt
     2881 tgagattcaa ccagccataa aaaatggaga gcgagttcct gcgacaacga atggaccatt
     2941 taaagtaaat ggtaagtgga catccggcac tcattataca ggtggttcca aacgtctaga
     3001 tgccggtatt attgatatca catctcctac aaaccaatat ggattacatc cagttattga
     3061 aggatttgat ataacactta ataaaacaaa accatc
//
LOCUS       DAZFKF010000243         3068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFKF010000243 DAZFKF010000000
VERSION     DAZFKF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.245"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(237..815)
                     /locus_tag="ACHKA1_005345"
     CDS             complement(237..815)
                     /locus_tag="ACHKA1_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HFO5682855.1"
                     /translation="MSDGRYKTNPFIPDMIIPIKDKQVRLSVLGREQNVLVNQATGEI
                     HGTHVTTYKRVDGEQFVKLFTANIALTFDLSAQGIKAFNVLMWAIQNKSLAKDEVYLD
                     THTREDFLAAHNDTDKPIKLSQPTFARGLAELTKAQIIAKTVRQGVYWINPNFAFNGD
                     RIAFTTLIERKRKNENETNAIQDGANAANETY"
     gene            complement(1015..1194)
                     /locus_tag="ACHKA1_005346"
     CDS             complement(1015..1194)
                     /locus_tag="ACHKA1_005346"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682856.1"
                     /translation="MGYHYQHQKIAFWCSVGLVKRIFEQNRNGLADNMREEVPLAAAP
                     ATERSERVGEEAEES"
     gene            1240..1347
                     /locus_tag="ACHKA1_005347"
     CDS             1240..1347
                     /locus_tag="ACHKA1_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rep protein"
                     /protein_id="HFO5682857.1"
                     /translation="MPTDARQGMEGAKNAYRAFQEVRRADTPNQGLSGR"
     gene            2736..>3068
                     /locus_tag="ACHKA1_005348"
     CDS             2736..>3068
                     /locus_tag="ACHKA1_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682858.1"
                     /translation="MIYRLATPEDYEYIPEINLWELSFDKRPVRGVRCEDPVIGSQIY
                     NKTRQKFILHKQTEKRRKQKFFRLKNISWDGIFDWCLSKGTPEECDLIIQLYYAKDKD
                     EHYSILNKL"
BASE COUNT          832 a          650 c          731 g          855 t
ORIGIN      
        1 aaaaaacacg aaggtttttt ccatgagtca gacttatatt tatggatatg tattttcttt
       61 atgagtggga ataaaatcgc atcctgcgat aaattaaacc tagtaaaaac aaaatgttaa
      121 tacatattga cacccccccc accatctaaa gaacgccgag gatatcaata tgtccgaaaa
      181 tgatgactga aatgttcact gtcgtgagtt ttttgattct gtttctttat atcttactaa
      241 taggtttcat ttgcggcgtt tgctccatcc tgtatcgcat tggtttcgtt ctcatttttt
      301 ctctttcgtt caatgagagt tgtaaatgct attctgtcac cattgaaagc aaagttagga
      361 tttatccaat acacaccttg tcgtacggtt ttagcgataa tttgagcctt cgtaagttca
      421 gctaatcctc ttgcaaatgt cggttgactt aacttaattg gtttatctgt atcgttgtga
      481 gcggctagaa aatcttctct cgtgtgagtg tctaaataaa cttcatcctt tgccaaagat
      541 ttattttgta ttgcccacat caaaacatta aacgccttta taccttgtgc tgataaatca
      601 aaggttaatg ctatatttgc cgtgaatagt tttacaaact gctcaccatc aacccgttta
      661 tatgttgtta cgtgcgttcc gtggatctca ccagtagcct gattgaccag tacattttgc
      721 tctcttccta aaacagacaa ccgaacttgt ttatctttta tcggaatgat catgtctggt
      781 ataaatgggt tcgttttgta tcgaccgtcg ctcataaaaa gcactcattt tgatagacct
      841 ctttcaatct tagatacccg gtctatcaaa atcaactgaa attttgatag accgtctgcg
      901 gacgtctgat agaccgtcta acacgatctg aaaattggat ccccatttca tgcgggttac
      961 gagaaattcc cttcttagtt cttatacggt ggtgcgtaag tgctcatttt cgcattagct
     1021 ctcttccgct tcctcgccca ctcgctcact acgttcggtc gctggggctg cggcgagcgg
     1081 tacctcctct cgcatgttgt cggcaagtcc gttgcgattc tgttcgaaaa tgcgttttac
     1141 gaggcctaca gagcaccaga acgcgatttt ctggtgctgg taatggtatc ccacgcctta
     1201 gcccttaaaa cgcgttcaga ggctctctga ggcgctaaaa tgccaacaga tgcccgtcag
     1261 ggcatggaag gcgcgaaaaa cgcttacaga gcgtttcagg aggtcaggcg cgccgatacc
     1321 cctaaccagg ggctttcagg tcgctaggaa ctgcgctagc cccctgcaaa gctcatttgc
     1381 acggaacagg cagccacacg actggtaggt ggtcgtagac cgttgatgtt gcagcgatgg
     1441 gggtctgggg ttctccccgg acggtttccg gttgcgctca aggcgcagcc ggaaccgtgg
     1501 cggcatccga tgatgtagta accgctgtat aagcagatgc tctaaatggg ggcacgttaa
     1561 aaagagattc cactttttcg gggttgtaag gccttgagtg gttgttttat gctcgctttt
     1621 gcctggttgg tgcggcctta aggctccagg catttgcacc tgtaacatga cagaacgtac
     1681 tttgcagact gccaatctgt gaggtgtttt gtctgagtcc atagctgaaa ctgaaacccc
     1741 ttattgccag taagggggag ttaacgaaac tgccaacttc gttaacagcg actgccgaag
     1801 ccgtatgcct gtaattgggc tggtacggct acgacgggcg ttcgggggtg aaactccccc
     1861 gtccgaccca atccgcgcag aagcgtgagg ctgggcgaca cggaaacggg aacgtaggtg
     1921 actacgtgat ccctgttgat cttgaacggg tgaaaatccc gaatatgggg tgcttgagag
     1981 ggtacaacat attcccgaca ggtgggctgc tgagggcatc gtgaggtgtc agggcgttga
     2041 ggggaaacct ggaaacgtcg gtactgcgag gggcaacctt tgcagatcgc tgtaaagctc
     2101 aagcctgtaa ttgcgtccga agaggtcgca ttcgggtaag tgataaccag ccatgactta
     2161 agtgtctggc tatggtgctg aaggcaaatt atgagtaagg gaggtgcttt ggaatctcgg
     2221 ccccctgaaa gccagaaatg gtaaggcgta gcctagtagt ttgtctgagc cagtggcggg
     2281 ttatcccgac aagatcagct aacgtgggaa gcactctgga aaatttcggg tggcaccgaa
     2341 attagattcc tgagtgtggg agagtgaacg agtaggcgca aaaatctaca ggcgatatag
     2401 ccgaagtctg tgggtgaatc tgagcatact ggcggcatcc ggtacagata agtagctgaa
     2461 agttcacagc agttgcaagg taggcaattt tttagcctgt tcagcgtatt ctgtctgctg
     2521 ataccagaca gaggactcaa acaaaaaacg tagacactat cttgattgtt accataacta
     2581 attgatgtga aagtacatat tcacaaaaaa cgcactctct ggtacttacc agatatgaat
     2641 actcatcacg tttgttgtga tcataaatat ggtactggca ctaccaatga taaacagctc
     2701 tcgaatgaga gctgtttcct atgaggggat gaaaagtgat ataccgactg gcaactccag
     2761 aagattatga atatatacct gaaattaatt tatgggagct ttcttttgat aaaagacctg
     2821 ttagaggggt acgttgtgaa gatcctgtga ttggctcaca aatatacaac aaaacccgac
     2881 aaaaattcat tttgcataaa caaacagaaa aaagaagaaa acagaaattt ttcagattaa
     2941 aaaatatatc atgggatggc atcttcgact ggtgtttatc aaaaggaaca ccagaagaat
     3001 gtgatttgat aattcagtta tattacgcaa aagataaaga cgaacattac tcaatcttaa
     3061 ataagttg
//
LOCUS       DAZFKF010000244         3068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFKF010000244 DAZFKF010000000
VERSION     DAZFKF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.246"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1853)
                     /locus_tag="ACHKA1_005349"
     CDS             complement(<1..1853)
                     /locus_tag="ACHKA1_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO5682859.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISD
                     SFGGPLSLLTLLKEEFLGIRDASESSEDSLSDTANALAENARNAGELGRFMSVARVAA
                     GGGVAVLAALAAAAWQAEQADRALLRSLTLTGGAAATTTAELWKMAGVISDEAGGGIR
                     QAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALAS
                     LNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQLDKAATPVEKIW
                     DDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWETT
                     KNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDARIRQGEMGYIRRSRATGV
                     SKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEAELRTLEKHSGA
                     NEKISRQRRDLWKAESQYAVLKEAATKRQLSGQEKSLLAHEKETLEYKRQLAELGDKV
                     EYQKRLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEEQRLRDV"
     gene            complement(1834..2247)
                     /locus_tag="ACHKA1_005350"
     CDS             complement(1834..2247)
                     /locus_tag="ACHKA1_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5682860.1"
                     /translation="MGRADWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGDGGRD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
     gene            complement(2274..2690)
                     /locus_tag="ACHKA1_005351"
     CDS             complement(2274..2690)
                     /locus_tag="ACHKA1_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5682861.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYIKFVSDAEQQETTKHD
                     AVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNDADPEQTGKAEATEPVTSKKHSKAS"
     gene            complement(2707..>3068)
                     /locus_tag="ACHKA1_005352"
     CDS             complement(2707..>3068)
                     /locus_tag="ACHKA1_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO5682862.1"
                     /translation="VTAKEVITRTVKITNIGRPSLAEDRGEITPVTGITVTPLTGNVA
                     KGQSTTLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKGVAAGKAQIPVVTGN
                     GEFAAVAEITVTDGAAG"
BASE COUNT          601 a          990 c          726 g          751 t
ORIGIN      
        1 tacacgtcac gaagacgctg ctcttcagac tcccgctgtg cctgacggtc agtgagacca
       61 cgggcttttg cgctgatggc ggcctgcttc gcgctctgct gctcttcaaa ccgcaccgcc
      121 tgctgtgcca gctcattcag ccgtttctgg tattcaacct tgtcgcccag ctcagccagc
      181 tgacgtttgt actccagcgt ctctttctca tgggccagca gggatttttc ctgcccggat
      241 aactgtcgtt tcgtggcagc ctctttcagg accgcatact gactttccgc cttccataaa
      301 tcacggcgct gccggctgat tttctcattc gcaccgctgt gtttttccag cgtcctgagc
      361 tcagcttcaa gggcaagcag agcctctctt gcctgctcct cttccctctc cccggcagag
      421 cgcgttttcg gtgatgtatg ctttttacct gtcagctctt cagccagacg gctgacggct
      481 tcctgctgcc ccggaccttt gctgacgcct gttgcacgcg aacgcctgat ataccccatt
      541 tccccctggc gtatacgcgc atcccgttcc gcaatggatt ttctcagcgc cagttcatcg
      601 cgttttgttt tctcaataaa tacgcggttc tcttctgcca gttcaccaaa caacgcacca
      661 acgccgggca cattctttgt cgtttcccag gctgactgaa taaattcagc cagcgccaga
      721 tccccctgca caagcagcag cttcacctgt tcaacggttc cggccaccac gtcagtgatc
      781 agactgagtg cccccagagt atgatcacct atccatgccc atgcgtcaga agtccaggtt
      841 ttaacatcgt cccagatttt ttccaccggc gtggccgctt tatcaagttg ctccagacgt
      901 gcattcatga catccgcaaa cagggacatc gcctccgtca ccgcagcctg tttacctttc
      961 gtgcgctcaa gctcatcaat atggcgtaac tgggaaacgc tcaggaagtt atactgctga
     1021 ttcagggagg ccagcgcctt caccggatct gctgcaatcc cttcaaaggc tttttccacc
     1081 ttcccggcat cgtcccccac cgtctgcagc catctctgag aggtttcccc catgatccgt
     1141 agctgcccgg cggtatattt cccgctttct gccagacggg ccagattttc tgccgcctgt
     1201 ctgataccac caccggcttc atcgctgatc accccggcca ttttccacaa ttctgccgtt
     1261 gtggtggcag ccgcccctcc ggtcagggtc agtgaacgta ataaggcccg gtcagcctgc
     1321 tctgcctgcc aggcggcggc agcaagcgcg gccagtacgg caaccccgcc acctgccgcc
     1381 acacgggcca ccgacataaa tcgccccagt tcaccggcat tccgggcatt ttcagccaga
     1441 gcatttgccg tatctgacag cgaatcctct gatgattcag aggcatccct gatcccgaga
     1501 aattcctctt tcagcagggt aagcaggctg agcggtccac cgaatgaatc gctgatctgc
     1561 cccccctgct gcagcatgat aaggaaggga ttctgaccac cggcaagctg agtgacaata
     1621 tccgtgaact gtgcgggcag tgtgcgcatg gcagccttat actgtccgac tgatatcccg
     1681 gctttttgtg cagccagcgc ctgtcggctc agcccctgtt caacagtact ggcggttttt
     1741 ctggcatccg cctccagact ggaaaaatga cggcgtaccc gggccatctg ttcatcaaac
     1801 cgggccgcat ccagactcaa atcaataacc agatcacccg ctggctggga catatctcac
     1861 acctccggaa atccccgctg aagccatcat taatgcaaca tcatcctcgc tgacatccac
     1921 cacatccgca gaaggtgaaa tatcgcgtcc cccgtcccct ccgaaccgga cgcctccggc
     1981 aactcctgtc gctttctgca tcagcatttc ttcctcgtcc ggcatctccg tctgctcttc
     2041 ctcacacgct ggcgcaagca gactgaaatc cgccggatgc atatccggat cgccaaaaaa
     2101 caggctgagt acggcgtaca tcagcccgga aaaatgagcg tccagttggg tatcctgaaa
     2161 ataatgcgtg cagtaaaaac gtcgccagtc ggcatattcg gtggatgtca tcccggcaag
     2221 catggcgcgc cagtcggctc tccccatctc acgcgccagt ttcaggacaa agttcagctc
     2281 gccttcgaat gcttttttga tgttaccggc tcagtcgctt ctgctttccc ggtttgttca
     2341 ggatcggcat cgttatccag catacctgaa agataaagca cccggttcgt tgcctgattc
     2401 agtgcatcag caggccatcc cagcatcact tcacggcgga tctgctgcat ctctgtctcc
     2461 ggagaggcca gagtgccttt gagggaatgg gaatgccata gcgacatcgc cacaagcagg
     2521 gatgccgttt ccagatatcg ctggttaatg tgcacggcat catgcttcgt tgtctcctgt
     2581 tgttctgcgt ctgaaacaaa ctttatataa tcaaaccgct gcagcgcgga cagttccgac
     2641 agcgtgacgg acacaccgtt atattcaaat tgttctgttt tcaggaacat gcttgatctc
     2701 ctcccctcag cccgcagcgc catccgtgac ggtgatctcc gccaccgccg caaactcacc
     2761 gttgccggtg acaacaggga tctgcgcttt acctgccgca acacctttca cggtgatcgt
     2821 gttccctttc acggtaatgg tggcgaaatt ctgatttgcc gacgttgcgc ggaatgtttt
     2881 atccgttgcg ccttccggct gaacggccac agtcagggtg gtgctctgac cttttgccac
     2941 attccccgtt agtggcgtca cggtaatacc ggtgaccggt gtgatttctc cccgatcctc
     3001 cgccagcgac ggacgaccaa tattggtgat ttttaccgta cgggtaatca cttctttggc
     3061 agtcaccg
//
LOCUS       DAZFKF010000245         3067 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFKF010000245 DAZFKF010000000
VERSION     DAZFKF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.247"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(44..367)
                     /locus_tag="ACHKA1_005353"
     CDS             complement(44..367)
                     /locus_tag="ACHKA1_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682863.1"
                     /translation="MDCTILLKIISVKEITKNSPLWSHYLARYVDSGYQAEFECEGTA
                     DKWDIYDEWDIYESIADYFDVVGDFNCDVRWSGRKILIKGTCVFPLGEPVKGIHNIPL
                     WIDVE"
     gene            complement(400..1302)
                     /locus_tag="ACHKA1_005354"
     CDS             complement(400..1302)
                     /locus_tag="ACHKA1_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO5682864.1"
                     /translation="MSRREKLLKQDLVAHIRKDKGSFKKLSDYTRIMHRFAESLAKMN
                     VQISSAAQVKVRHIELYMRSRNNVSGRTRENEMSAIRVMLRQAGKYKMADPEHPRLSN
                     KALGISGSSRKGTKKLITDERFREILQRVEAKDKGVAAVVKLSRYLGLRNEEAIQSAK
                     SLRTWKKALQRGDEKIRVIFGTKGGRERMTTVVDRDNVLDAVNYALKYASEHNGRLID
                     RDNLKSAMDYYLNTLRRYGELKGRDETAHSFRYSYAASAMKYHIMNGYSYKEALALTS
                     IDLGHGDGRGRYIEQVYCQLLGYE"
     gene            2617..>3067
                     /locus_tag="ACHKA1_005355"
     CDS             2617..>3067
                     /locus_tag="ACHKA1_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682865.1"
                     /translation="MCDFIKSCYAAPRINKMADFFSSTASGVKHKPLKHVKIDNRLFP
                     SPEYIIFSDYLTKNSGPFVNHYFCSIPYSKEEECRLGVTIMKYASHRNKPLSLWCLGM
                     AEGAMARTISQLSKGQVLSLTTSPTKENEPVFFSHGSHIHSYFICSPF"
BASE COUNT          915 a          718 c          556 g          878 t
ORIGIN      
        1 tataagcctg tatattttta aatcacaaaa gatgaatgtc aagttattca acatcaatcc
       61 aaagaggaat attatgtatt cccttaacgg gttctcccag aggaaaaaca caggtccctt
      121 taatcagtat ttttcttccc gaccatctga catcacagtt aaaatctccc accacatcaa
      181 aataatccgc aatggattca tagatgtccc attcatcata gatatcccat ttatctgctg
      241 ttccttcaca ttcaaactcg gcctgatatc cggaatcaac atatcgcgcc agatagtgtg
      301 accacaaagg cgaatttttt gtaatctctt ttacagagat tattttcagc aaaatagtac
      361 aatccataat atttacccgt acgaaatcgc catgagttat tactcatagc ctaacaactg
      421 acaataaacc tgttcaatat atcgtcctct gccatcacca tgaccaagat caattgatgt
      481 cagtgcaagt gcctccttat aactgtaacc gttcataata tgatatttca tggcacttgc
      541 agcgtaactg taccgaaaac tgtgtgcagt ctcatctctg cctttcagtt ctccataacg
      601 acgtaatgta ttaagataat aatccattgc cgacttaaga ttatctctgt ctataagacg
      661 tccattatgt tcactggcat atttgagtgc ataattaaca gcatcaagaa cgttatcccg
      721 gtcaacaacc gttgtcattc tttcccgtcc ccctttcgta ccaaaaatca ctcttatttt
      781 ttcatctccc cgttgtaacg cttttttcca ggttcgtaat gatttggccg actggatagc
      841 ttcttcattt ctcagaccga ggtaacgtga cagtttaaca acagcagcca cccctttatc
      901 ttttgcttcc acacgctgaa gaatctcccg aaaacgctca tccgtgatta atttttttgt
      961 tcccttacga ctggagccgg aaatgcctaa cgctttattg ctgagacgag gatgttcggg
     1021 atcagccatc ttatatttac ctgcctgacg taacataacc cgtattgcag acatttcatt
     1081 ttcccttgtt cgcccagaaa cattattccg gcttctcata tatagctcaa tatgccggac
     1141 ttttacctgc gcggcagaac tgatctgtac attcattttt gccagacttt cagcaaagcg
     1201 gtgcatgatc ctggtgtaat cactcagctt cttgaagctg cctttgtctt ttcgaatgtg
     1261 agcaaccaga tcctgtttca gtaatttttc acgtctggac atcaacttct cctgcacata
     1321 ataattttta ccttaaacag ctgtacgttc ggcagatgag tttttctgtt gtgccgtgaa
     1381 cacaacagaa aaactcatct gcatttctcc ctgacgacac ggttaaacgt tgcgatcctt
     1441 cgggtagtga ctgcctgtgc gccagggtag cgcagattgt ttaggtattg aggggtaaca
     1501 aatcggtgtt gcacaccgac tgcagcattt gctgtacctg tctccaggtc gcccccgaca
     1561 ccacctctaa taagtggtat cgccgattcg gcatctcact acataaaact ccttaaaaaa
     1621 acaattgatt ctgcaaaaaa ccatttatgg acgttattca caaaatcgtc cgtaaatttg
     1681 cagaagaggt tgaaaaaaca ctctcacgcc gggtcacttc ttcaaatgtt aaaaaacatc
     1741 acgtgaaacg gcagttcgtg cgggttcgtt aagcagacgc gcgtgcgtca ctttgtcatc
     1801 gtgacacgct gacgaaagtg acgccgcgca gcttcagacg ccagatacac gcgtaagcga
     1861 ccaaatttca aatggtctta tacagcgcgc acagtcgcag gaagtgctgg caaacaaatg
     1921 aaaagaataa aagaagtcaa taccgccatt acgcagtaag aacattccgg catcaccgga
     1981 tggataagga tcacacaacc actgctgtgt attgcccgta ataaagggcg cggttaaaag
     2041 aaaccttcat cgtcatgact gacgatatct gctctcaaag gtgggcagat ttaccaccac
     2101 cagaaacatc ctggctgcac atcccctttc aggttacggg ggggttgatc acccgaaggc
     2161 gttcctctca gatcaacgga acattggtgc ccaccgaagc gggatgtggt cacccgaagg
     2221 cgctcctctc agaccactta tggagcattg gtggtcgccg aagcgacaga taatgcattc
     2281 tgagcatatc agagcgcata aaaggcgtca attatccatg ctccctcacg aaacagaagc
     2341 tgaactctta acacctgcta tcggaactca ctgaataaac aactaaaaag gacattgtga
     2401 aaattaacat caacagtatg taaagtgatt tgttttgtct taaatgggtg aatgtaaaaa
     2461 aatattctat acaatccaca catccttgta tcatatatca agtaaacatc cacagagata
     2521 acagcttatt tcaaataagc agattctgtt tgatacttca gaaaaatagt atatgctcct
     2581 gatttactga tgttataagt tatacaggat agttaaatgt gtgattttat aaaatcatgt
     2641 tatgcagcac caagaattaa caaaatggct gattttttct catcaacagc atctggagta
     2701 aaacataaac ctttgaagca tgtaaaaatt gataacaggc ttttcccatc tcctgagtat
     2761 ataatttttt ctgactatct tacgaaaaac agtggtccat ttgttaacca ctacttttgc
     2821 agtattccat acagcaaaga agaagaatgc aggctgggtg tgactattat gaaatatgca
     2881 agccatcgaa ataagccatt gagcttatgg tgtcttggaa tggcagaagg agccatggcc
     2941 agaacaattt ctcaactatc aaaaggacag gttttatcat taacaaccag cccaacaaaa
     3001 gaaaatgaac ctgtattctt tagtcatggt tctcatattc actcttactt tatttgctcc
     3061 ccattct
//
LOCUS       DAZFKF010000246         3064 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFKF010000246 DAZFKF010000000
VERSION     DAZFKF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.248"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1886)
                     /locus_tag="ACHKA1_005356"
     CDS             complement(<1..1886)
                     /locus_tag="ACHKA1_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO5682866.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFL
                     IMLQQGGQISDSFGGPLSLLTLLKEELLGIRDASESSEDSLSDTANALAENARNAGEL
                     GRFMSVARVAAGGGVAVLAALAAAAWQAEQADRALLRSLTLTGGAAATTTAELWKMAG
                     VISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEG
                     IAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQL
                     DKAATPVEKIWDDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLAL
                     AEFIQSAWETTKDVPGVSALFGELAEENREFIEKTKRDELALRKSIAERDARIRQGEM
                     GYINRSRATGVSKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEA
                     ELRTLEKHSGANEKISQQRRDLWKAESQYAVLKEAATKRQLSGQEKSLLAHEKETLEY
                     KRQLAELGDKVEYQKRLNELAQQAARFEEQQSAKQAAISAKARGLTDRQAQRESEEQR
                     LRDV"
     gene            complement(1936..2238)
                     /locus_tag="ACHKA1_005357"
                     /pseudo
     CDS             complement(1936..2238)
                     /locus_tag="ACHKA1_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(2265..2687)
                     /locus_tag="ACHKA1_005358"
     CDS             complement(2265..2687)
                     /locus_tag="ACHKA1_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5682867.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAAQQETTEHD
                     AVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEPTGKAEATEPVTSKKHSKAS"
     gene            complement(2703..>3064)
                     /locus_tag="ACHKA1_005359"
     CDS             complement(2703..>3064)
                     /locus_tag="ACHKA1_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO5682868.1"
                     /translation="VTAKEVITRTVKITNIGRPSLAEDRGEITPVTGITVTPLTGNVA
                     KGQNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKGVAAGKAQIPVVTGN
                     GEFAAVAEITVTDGAAG"
BASE COUNT          587 a          985 c          740 g          752 t
ORIGIN      
        1 tacacgtcac gaagacgctg ctcttcagac tcccgctgcg cctgacggtc agtgagacca
       61 cgggcttttg cgctgatggc ggcctgcttc gcgctctgct gctcttcaaa ccgtgccgcc
      121 tgctgtgcca gctcattcag gcgtttctgg tattcaacct tgtcgcccag ctcagccagc
      181 tggcgtttgt actccagcgt ctctttctca tgggccagca gggatttttc ctgcccggat
      241 aactgccgtt tcgtggcggc ctctttcagg accgcatact gactttccgc tttccataaa
      301 tcacggcgct gctggctgat tttctcattc gcaccgctgt gcttctccag cgtcctgagc
      361 tcagcttcaa gggcaagcag agcctctctt gcctgctcct cttccctctc cccggcagag
      421 cgcgttttcg gtgatgtatg ctttttacct gtcagctctt cagccagacg gctgacggct
      481 tcctgctgcc ccggaccttt gctgacgcct gttgcacgcg agcggttgat gtaccccatt
      541 tccccctggc gtatacgcgc atcccgttcc gcaatggatt ttctcagcgc cagttcatcg
      601 cgttttgttt tctcaataaa ttcccggttc tcttctgcca gctcgccaaa cagcgcactg
      661 acgccgggga catcttttgt cgtttcccag gctgactgaa taaattcagc cagcgccaga
      721 tccccctgca caagcagcag cttcacctgt tcaacggttc ctgccaccac gtcggtgatc
      781 agactgaggg cccccagagt atgatcacct atccatgccc atgcgtcaga agtccaggtt
      841 ttaacatcgt cccagatttt ttccaccggc gtggccgctt tatcaagttg ctccagacgt
      901 gcattcatga catccgcaaa cagggacatc gcctccgtta ccgcagcctg tttacctttc
      961 gtgcgctcaa gctcatcaat atggcgtaac tgggaaacgc tcaggaagtt atactgctga
     1021 ttcagggagg ccagcgcctt caccggatct gctgcaatcc cttcaaaggc tttttccacc
     1081 ttcccggcat cgtcccccac cgtctgcagc catctctgag aggtttcccc catgatccgt
     1141 agctgcccgg cggtatattt cccgctttct gccagacggg ccagattttc tgccgcctgt
     1201 ctgataccac caccggcttc atcgctgatc accccggcca ttttccacaa ttctgccgtt
     1261 gtggtggcag ccgcccctcc ggtcagggtc agtgaacgca acaaggcccg gtcagcctgc
     1321 tctgcctgcc aggcggcggc agcaagcgcg gccagtacgg caaccccgcc acctgccgcc
     1381 acacgggcca ccgacataaa tcgtcccagt tcaccggcat tccgggcatt ttcagccagt
     1441 gcatttgccg tatctgacag cgaatcctct gatgattcag aggcatccct gatcccgaga
     1501 agttcctcct tcagcagggt aagcaggctg agcggtccac cgaatgaatc gctgatctgc
     1561 cccccctgct gcagcatgat aaggaaggga ttctgaccac cggcaagctg agtgacaata
     1621 tccgtgaact gtgcgggcag tgtgcgcatg gcagccttat actgtccgac tgatatcccg
     1681 gctttttgtg cagccagcgc ctgtcggctc agactctgct cagcagcatc agcctgtttt
     1741 ctgaaagact gactgacttt cccggacatc aaatcagcaa gcgcactggt ttcacccagc
     1801 tcttttttta cccgcgctgc ctcttcagaa aaacgggcag aatccagtgt cagcacggct
     1861 gtcagatcgg caaaattacc tgccatcgtg gtcacctcct gaaatgtcct ctgatgccat
     1921 caataactgt cacaacctcc ttccctcctc cccgaaacgg actccaccgg cgaggcccgc
     1981 cgccttctgc atcagtacat cattttcgtc cggcgtctcc gcctgccctt cctctgcctc
     2041 cggagcgaac aggctgaaat ccgccggatg catatccgga tcgcaaaaaa acaggctgag
     2101 tacggcgtac gtcagcccgg aaaaatgcat atccagctgg gtatcctgaa aataatgcgt
     2161 gcagtaaaaa cgtcgccagt cggcatattc ggtggatgtc atcccggcaa gcatggcgcg
     2221 ccagtcggct ctccccatct cacgcgccag tttcaggaca aagttcagct cgccttcgaa
     2281 tgcttttttg atgttaccgg ctcagtcgct tctgctttcc cggttggttc aggatcggca
     2341 tcgtgccggt tatccagcat acctgaaaga taaagcaccc ggttcgttgc ctgattcagt
     2401 gcatcagcag gccatcccag catcacttca cggcggatct gctgcatctc tgtctccgga
     2461 gaggccagag tgcctttgag agaatgggaa tgccatagcg acatcgccac aagcagggat
     2521 gccgtttcca gatatcgctg gttaatgtgc acggcatcat gctccgttgt ctcctgttgt
     2581 gctgcgtctg aaacaaactt catataatca aaccgctgca gcgcagacag ctccgacagc
     2641 gtgacggaca ccccgttata ttcaaattgt tctgttttca gaaacatgct ttatctcccc
     2701 cctcagcccg cagcgccatc cgtgacggtg atctccgcca ccgccgcaaa ctcaccattg
     2761 ccggtgacaa cagggatctg cgctttacct gccgcaacac ctttcacggt gatcgtgttc
     2821 cccttcacgg taatggtggc gaaattctga ttcgctgagg ttgcgcggaa cgttttatcc
     2881 gttgcgcctt ccggctgaac ggccacggtc agggtgatat tctgaccttt tgccacattc
     2941 cccgttagtg gcgtcacggt aataccggtg accggtgtga tttctccccg atcctcagcc
     3001 agcgacggac gaccaatatt ggtgattttc acagtgcggg tgatcacttc tttggcggtc
     3061 acgg
//
LOCUS       DAZFKF010000247         3009 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFKF010000247 DAZFKF010000000
VERSION     DAZFKF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.249"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            125..763
                     /locus_tag="ACHKA1_005360"
     CDS             125..763
                     /locus_tag="ACHKA1_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5682869.1"
                     /translation="MRTRIDYLADKYSFTELNERPHLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLIRTPQLIAALREEWDIGQKNVLFNDKRFGCVYSLKS
                     SLSGVPDTYRYHLSHRIRRVVANENTSLPYQQIAREVKAPRERLKYALETGLQVTALD
                     GLFWFGSQRIAADIQRLRKSGMRITTSAISVYDSITVTRRLCPVYRIVANNK"
     gene            1100..2719
                     /locus_tag="ACHKA1_005361"
     CDS             1100..2719
                     /locus_tag="ACHKA1_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO5682870.1"
                     /translation="MARIPTKKAKAKKGFEETLWDAANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFETRRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPANTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTAEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRQNLEALGYGE"
     gene            2709..>3009
                     /locus_tag="ACHKA1_005362"
     CDS             2709..>3009
                     /locus_tag="ACHKA1_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO5682871.1"
                     /translation="MGSKWEKIKLKEVVDIITTKVDVSQISLCDYISTENMLTNFGGI
                     SIANSKPSTGKITKFHSGDILFSNIRTYFKKLWLADRTGGCSNDVIVFRPKKHINS"
BASE COUNT          859 a          668 c          753 g          729 t
ORIGIN      
        1 tttatgtcgc aggttattgc aacaatcatc cggtgattgt cgggaggaat atcaatgctt
       61 ccgttaattt ttactggctg ataaagtttt gtctgctgaa tctttatacg gaattctttt
      121 cagtatgcgt acacgaattg actatcttgc agataaatac agttttactg aactgaatga
      181 acgtccacac cttcgccggc agtggcagga tgttctggag gagtgtcggc agacagaggc
      241 cgggccagaa gaacggctgc ggattgccct gttgaatgtg gattacgtca ccagttttga
      301 acttccattt cggttgctgc ttatccgtac accacaactg attgctgcgt tgcgtgaaga
      361 atgggacatc ggccagaaaa atgtgctgtt taacgataaa cggtttggtt gcgtgtacag
      421 cctgaagtcc agtctttctg gtgtaccgga tacataccgg tatcatctgt ctcaccgtat
      481 tcgtcgtgtg gttgcgaatg aaaacacatc attgccatat cagcagattg cccgggaagt
      541 gaaagccccc cgggaacgac tgaagtatgc gctggagacg ggattgcagg tgaccgcact
      601 ggacggcttg ttctggttcg gtagccagcg cattgctgca gatatacaga gactgagaaa
      661 atctggtatg aggattacca cttctgccat aagcgtgtat gactctatta ccgtaacccg
      721 gagattgtgc ccggtatacc ggattgtcgc gaataataaa taaacggtaa tgctgagttt
      781 ctccattacc attgcaaatg actcaccaga gcagactgaa cagcgcagaa gtgggattgt
      841 ggatacgtga agtgagagta aggggaaaat ccacaataat catctatcga acagggaggc
      901 gaactttaca cgatggtttt ccgggagtgc ttacccgggg ttcctcacct ctggctaatc
      961 tctggattga gtcgcgatac tccaacaaaa gcaacaagct aacgcagcaa gaagttaacg
     1021 ctcatcgaga gtaaaatgca cacttttatg gcttactcgt tacaataaca gccagtttgt
     1081 tcagaaaacc ggattcagta tggccagaat accaaccaaa aaagctaaag caaaaaaagg
     1141 gtttgaagaa acattatggg atgccgcaaa tcagcttcgc ggcagcgttg agtcctccga
     1201 atacaagcac gtggtgttga gcctcgtgtt cctgaaattc atcagcgata agtttgaaac
     1261 acgccgcaaa aaaatgattg ccgatgggca ggcagatttc cttgagatgg aagtgttcta
     1321 ccagcaggac aacattttct acctgccgga agaggcgcgt tggtcattta tcaaacaaaa
     1381 tgcaaaacag gacgatattg cggttcgtat tgacaccgcc ctctcgacca ttgagaaacg
     1441 taacccaacc ctgaaaggtg cgctgccaga caactacttc agccgtcaga atctggaaac
     1501 caaaaaactg gcatcactga ttgataccat cgacaacatc gaaacgctgg cacacgagac
     1561 tgacgttgaa acgttatcga aagaagacct ggtcggacgc gtttatgaat acttcctcgg
     1621 taagtttgcc gccactgaag gcaaaggcgg tggtgagttc tacacgccaa aatgtgtggt
     1681 cacgctgtta actgaaatgc tcgaaccctt ccagggcaaa atttatgacc cgtgctgcgg
     1741 ctcggcagga atgttcgtgc agtcggtgaa gtttgtcgag agccatcagg gtaaaagccg
     1801 tgatatcgcg ctgtatggtc aggagctgac agccacgacg tataaactgg caaaaatgaa
     1861 cctcgctatt cgcggtcttt cagctaacct cggcgaacgc ccggcaaaca ctttctttag
     1921 cgaccagcac ccggacctga aagctgacta tattctggcg aacccgccgt tcaacctgaa
     1981 agactggcgt aacgaagcag aattaaccaa agacccacgt tttgccggtt atcgtatgcc
     2041 gccaaccggt aacgccaact acggctggat tttgcatatg ctctccaagc tgtcggctaa
     2101 cggcacagcg ggttttgtgc tggcaaacgg ttcgatgagt tctaacacca gcggtgaagg
     2161 cgagatccgt gcacagatga tcgaaaatga tctgatcgac tgcatgattg ctctgccagg
     2221 tcagttgttt tacaccacgc agatcccggt gtgtttatgg tttatgacca aatcgaaggc
     2281 tgccgatccg gccaaaggtt atcgtgatcg tcagggcgag acgctgttta ttgatgcgcg
     2341 taacctcggc accatgatta gccgcacaac taaagagtta acagcggaag atattgccac
     2401 aatcgccgat acttaccatg cctggcgtag cacgccagaa gaactggctg cacggattgc
     2461 gcgtggtgac agcaagctgg aaaaatatga agaccaggca ggcttctgca aagttgcgac
     2521 cctgcaagat attaaagata acgactacgt tctgacaccg ggccgctatg tgggtgcagc
     2581 cgagcaggaa gaagacggcg tggcatttga gaccaaaatg cgtgaattgt cgaagacgtt
     2641 gtttgagcag atgaagcagg cggaagaact ggatcgtgcg attcgccaga atctggaggc
     2701 gctgggttat ggggagtaaa tgggagaaaa taaaacttaa agaagttgta gatattatca
     2761 ctactaaagt tgatgtatcg caaattagtc tttgcgatta catatcaact gaaaatatgc
     2821 ttaccaattt tggaggtata tcaatagcaa atagtaaacc tagcacaggg aaaataacaa
     2881 aatttcattc tggagatatt ttattctcga atatcagaac atattttaaa aaactatggc
     2941 ttgcagatcg aactggtggc tgttctaacg atgtaattgt attccgtccc aaaaaacata
     3001 ttaattcta
//
LOCUS       DAZFKF010000248         3004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFKF010000248 DAZFKF010000000
VERSION     DAZFKF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.250"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            173..1384
                     /gene="bluF"
                     /locus_tag="ACHKA1_005363"
     CDS             173..1384
                     /gene="bluF"
                     /locus_tag="ACHKA1_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO5682872.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMAHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANGLVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            1579..2310
                     /gene="bluR"
                     /locus_tag="ACHKA1_005364"
     CDS             1579..2310
                     /gene="bluR"
                     /locus_tag="ACHKA1_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO5682873.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2531..2935
                     /locus_tag="ACHKA1_005365"
     CDS             2531..2935
                     /locus_tag="ACHKA1_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO5682874.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
BASE COUNT          883 a          632 c          644 g          845 t
ORIGIN      
        1 attgcatcat ttaactgaca acctatacaa gatttttcgc caactcgtgt tcaacttttt
       61 ccattttcta tcaatgtaaa gaaacagtta tgttttggta ttaaaagaaa gacaccggaa
      121 gatatgtctg ttaccgtctt actctcgcct ctcccattac cctggacagt taatgcttac
      181 cacccttatt tatcgtagcc atatacgtga cgacgaacct gtcaaaaaaa tcgaagaaat
      241 ggtttcgata gcaaatcgca ggaacatgca gtctgacgta acagggatct tactgtttaa
      301 tggttctcat tttttccagc ttctggaagg tccggaagaa caggttaaaa tgatatatcg
      361 ggctatatgc caggatccac ggcactataa tattgttgag ctgctgtgcg attacgcgcc
      421 tgctcgccgt tttggcaaag cgggaatgga attatttgat ttgcgcctgc acgagcgaga
      481 tgacgtttta caggccgtat tcgacaaagg cacatcaaaa tttcagctaa cttatgatga
      541 cagagcgcta caattttttc gtacttttgt ccttgcaacc gaacaatcaa cctatttcga
      601 gatccctgcc gaagactcct ggctttttat cgctgacgga tctgataaag aacttgattc
      661 ctgtaccctt tcaccaacta taaacgacca ctttgccttt catcctattg tcgatccctt
      721 atcgcggcgg ataatcgctt ttgaagccat tgtgcaaaaa aatgaagaca gcccgtcagc
      781 tatagcggtt ggtcagcgta aagacgggga aatctacaca gcggatctta aaagtaaggc
      841 gcttgcattc gcgatggcac acgcacttga gctcggtgat aaaatgatat caatcaatct
      901 attacctatg accctggtta acgagcctga cgcagtctct tttttacttg atgaaataaa
      961 ggccaatggt ctggtgcctg aacaaatcat cgttgaattt actgaaagtg aagtcatatc
     1021 tcggtttgat gagtttgccg aagcggttaa atcgctaaag gctgccggta tcagtgtagc
     1081 aattgatcat tttggcgcag gttttgctgg tttgttactc ctgtcacgct tccagcctga
     1141 cagaattaaa atcagtcagg aattgattac caatgttcat aaaagcggac cacggcaggc
     1201 aattattcag gcgatcatga aatgctgtac atcacttgaa attcaagtct ctgctatggg
     1261 cgtggcaaca ccagaagagt ggatgtggct tgaatccgca ggaattgaga tgtttcaggg
     1321 ggatctgttt gctaaagcta aattgaatgg tatcccttcc atagcgtggc cagaaaaaaa
     1381 ataattttca gcacataatt tcagtaaatc atcgtgaaaa tgaatacgtt atgtttaaat
     1441 ttaatccaca ttaaaaataa tgtttcatcc aaattagctg aaatcttatt tatactttgt
     1501 acaattttag cgtatattgt acaaagtatt attgggtcgt ttacaggcga cggagatttg
     1561 tgaccgcaag gaggaattgt ggcttattac agcattggtg atgttgctga acgttgcggg
     1621 attaatcctg tcactctccg ggcctggcaa cgccgctacg gtttgttaaa accacagcgc
     1681 agtgaaggcg gacaccgact ctttgatgaa gaagacatac aacgtatcga agagatcaag
     1741 cgttggataa gtaatggcgt ccctgtaggc aaagttaaag cattactgga aaccaccagc
     1801 caggatacgg aagatgactg gagccgcctg caagaagaga tgatgtcaat tcttcgtatg
     1861 gctaatcctg ccaaactacg cgcgagaatt atttcactgg gtcgagagta cccagtagat
     1921 caattgatta atcatgttta ccttcctgtt cgccagcgtc tcgtgcttga tcacaacacc
     1981 tcccgcatta tgagcagtat gtttgacggc gcattaattg aatatgcagc agcctcgctt
     2041 ttcgaaatgc gccgtaagcc cggtaaagaa gccattctga tggcgtggaa tgttgaagag
     2101 agagctcgat tgtggctgga agcatggcgt ttatcattgt caggatggca tatttctgtt
     2161 cttgctgatc ccattgaatc accgcgcccg gaactgttcc cgacgcaaac attgattgtc
     2221 tggacaggca tggcaccaac gagaaggcaa aacgaacttt tacaacactg gggtgagcaa
     2281 ggttataaag tcatctttca tgccccctaa tttcaccttt atcccgcccc ttagacagaa
     2341 tgttataatc tgaacccaca acagcgtttg taccggagct ggtccattac aaaatgctca
     2401 ccgggagaat tcaggtaatc cccctttcac actttgtaca tatcacagcc aaaggacaga
     2461 ccagattgaa gtggtaaaac cgatcgtgca cactacgcag tctcacaact aacaagaggt
     2521 tattatcctg atggatcagg tcgttgtttt tcaaaagatg tttgagcaag tgagaaaaga
     2581 gcaaaacttc tcctggtttt attcagaatt aaaacatcac cgtattgcac attacattta
     2641 ttatctggct acggataaca tcagaattat tactcacgat gacacggttt tgttattaag
     2701 aggaaccagg aacctgttaa aagttagtac gaccaagaac cctgctaaaa taaaagaggc
     2761 cgcattgctt catatttgcg gaaaatctac atttcgggaa tactgttcaa cactggcagg
     2821 cgcgggcgtt ttccgttggg ttactgatgt taatcataac aaacgcagtt actacgccat
     2881 tgataatacg cttttatata ttgaagatgt agaaaataat aaaccattaa tctagctaaa
     2941 gttggatgct taagaaatgc ttcataattc agtaaggcat tagcataatg ggaataaaag
     3001 taca
//
LOCUS       DAZFKF010000249         2967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFKF010000249 DAZFKF010000000
VERSION     DAZFKF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.251"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2047
                     /locus_tag="ACHKA1_005366"
     CDS             <1..2047
                     /locus_tag="ACHKA1_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO5682875.1"
                     /translation="KSRKSVKSVEPDIADRANNWLKSYGLDYKLEQAPLNNEIDNALD
                     AYFSKNGGTGGNRPDVKLLVQDKYGKQYPVLIEYKGYKDRLIRLGSNGIIENKDARKE
                     WNFKNINGYAVNGAVHYANALLQFTNYPDIIAIGMTGWRDDDTGELHHEIGVWYVSKN
                     NLGAGQKVGEFTDLSFLADKNVDGFLNKIKLLNLPPEELEKIKASKEEEIDTRLSRLN
                     NDIYQNEKGLGESDRVYLVVATVIATLGIPGKLAPLDKKELTSSTEEDLRDGDIIFRK
                     IRNFLRLKAVPETKREMILRSLQNTLWTENINKPVNGESQLKRVFVKVVDDLGEYYKI
                     GLTTDFTGKLFNEMYRWLGFTQDKLNDVVLTPPYVATLLARLARVNKDSYVWDFATGS
                     AGLLVAAMNEMLIDARENIHSPNELQLKEAQIKAEQLLGLEVLSSIYMLAILNMILMG
                     DGSSNILNKDSLADFDGKYGFGKTGEKFPADAFILNPPYSAKGNGMIFVQKALSMMDK
                     GYAAVIIQSSAGTGKATEYNKKILKENTLLASIKMPADLFIGKSSVQTYIYVFQVKIP
                     HNAKQAVKFIDFSNDGYARSNRKKARNNLVDADRAKERYQEVVDLVHFGKGCLNIFTE
                     DEYFEGTIDPDSGEDWNQTRPVDTRPTLEDFKKTVGDYLAWEVSKLLQGRDASEKKQ"
     gene            2080..>2967
                     /locus_tag="ACHKA1_005367"
     CDS             2080..>2967
                     /locus_tag="ACHKA1_005367"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO5682876.1"
                     /translation="MKSGGKWEEYTIERLFSIQTPKKKFNANKLTFNGNYPYVARGSN
                     NNGIKGYIDEDELYLNPGNTISFGQDTATMFYQENAYFTGDKIKIFSLRHGELNSRIA
                     FFLISAMKKSFSTFSWGSSSFNENVLNKVKINIPTFNNVPAYNYMVKYIEELEAAHIE
                     ELEAYLIAAGLSDYQLTEKDYLILEHFKNIEFSGFPVTELFSVCNTGNILSRDIIENS
                     GDVPYLCASRENNSVSSYIAYDNSLLEKGNCIFIGGKTFVVTYQERDFFSNDSHNLRL
                     YVNNEDGRTKFAYLGIISCI"
BASE COUNT          934 a          531 c          685 g          817 t
ORIGIN      
        1 taaatcaaga aagtctgtaa aatctgttga accagacatt gccgaccggg ctaacaactg
       61 gctcaaatct tatggacttg attacaaact ggagcaggca ccacttaaca acgagatcga
      121 taacgcactg gatgcttatt tttcgaaaaa tggcggcaca ggcggtaatc gtcccgacgt
      181 aaaactcctt gttcaggaca aatacggcaa gcagtatccc gttctcatcg agtataaagg
      241 ctacaaggac aggcttatca gacttggcag caacggtatt attgagaaca aagatgccag
      301 aaaagaatgg aattttaaaa atattaatgg atacgcggtg aatggcgcag tccattatgc
      361 caacgcactt cttcagttca ccaattaccc tgatattatt gctattggta tgaccggctg
      421 gcgcgatgat gatacaggcg aactgcatca tgaaatcggc gtgtggtacg tatcaaagaa
      481 taatttagga gccgggcaga aggttggtga attcaccgac ttatcattcc ttgctgacaa
      541 aaatgttgat ggatttctca acaagataaa gctgcttaat cttcctcccg aagaactgga
      601 aaaaatcaag gccagcaaag aggaagagat agacacgcga ctttcccgtc tgaataacga
      661 tatttaccag aatgaaaagg ggcttggtga aagcgatcgt gtttaccttg ttgtggctac
      721 cgttattgca acgttgggga ttccgggaaa gttagcaccg ctggataaaa aagaactgac
      781 ctcttcaacg gaggaggact tgcgtgatgg tgatattatt ttcaggaaaa taaggaattt
      841 tttaaggctg aaagctgttc ctgaaacaaa aagagaaatg attttgcgct cgttgcaaaa
      901 tacgctgtgg actgaaaaca ttaacaaacc agtcaacggt gaaagccagt taaagcgcgt
      961 gtttgttaaa gttgttgatg atcttgggga gtattacaaa atcggactga ccaccgattt
     1021 tacgggcaaa ctgtttaatg agatgtatcg ctggcttgga tttacgcagg acaaactcaa
     1081 tgacgtcgtt ctgacaccac cttatgttgc cacattgctg gcacgactcg ccagagtaaa
     1141 caaagattcc tatgtgtggg attttgctac aggttctgcg ggattgcttg tggctgcaat
     1201 gaatgaaatg ctcattgacg ccagagaaaa tattcactca ccgaatgaac tacaacttaa
     1261 ggaagcgcaa atcaaggctg aacagcttct tggccttgag gtgttatcca gtatttatat
     1321 gctggccatc ctgaatatga ttttgatggg ggatggtagc tcgaatatcc tcaataaaga
     1381 ctcgctagcc gattttgacg gcaagtatgg atttgggaaa acaggagaaa aattccctgc
     1441 ggacgcattt attctcaatc ctccgtattc agctaaaggt aacggcatga ttttcgtgca
     1501 gaaggcgctg tcgatgatgg ataaaggcta tgctgcagtg attatccaga gttcagccgg
     1561 cacaggaaag gctacggagt acaacaaaaa aatactcaag gaaaatacct tgctggcaag
     1621 catcaaaatg cctgcggatc tgtttattgg taaatcaagc gttcagacgt acatttacgt
     1681 ttttcaggta aaaattccgc ataacgcgaa acaggctgtt aagtttatcg acttttccaa
     1741 tgacggctat gctcgctcta atcgcaaaaa ggcaagaaac aatcttgttg atgccgatcg
     1801 tgcaaaagag cgttatcagg aggttgtgga tctggttcac ttcgggaaag gttgccttaa
     1861 tatctttacg gaggatgaat attttgaggg aacaatcgat cctgatagtg gtgaggactg
     1921 gaatcagact aggccagtag ataccagacc gacactggag gattttaaaa agacggttgg
     1981 cgactatctt gcgtgggagg tgtcaaaact tcttcagggg cgtgatgcgt cagaaaaaaa
     2041 gcagtaaact cccgtcttgc agagcttgag cgggagttta ttaaatctgg tggtaaatgg
     2101 gaagaataca caatcgaaag attgttcagt attcagacac ctaaaaagaa atttaatgcc
     2161 aataaattaa catttaatgg caactatcct tatgttgcca gaggaagtaa caataacggc
     2221 ataaaaggat atatagatga agatgaatta tatctcaacc cggggaatac aattagcttt
     2281 ggtcaggata ctgcgactat gttctatcag gaaaacgcat atttcacagg tgataaaata
     2341 aagatttttt ctctcaggca tggagagtta aattcgcgaa ttgctttttt tcttatatca
     2401 gcaatgaaaa aatctttttc aacgtttagt tgggggagta gctcttttaa tgagaatgtt
     2461 ctcaataagg taaagataaa tatacctacg tttaataatg ttcctgcata taattatatg
     2521 gtaaagtata tagaagagct ggaagcggcc catatagaag agctggaagc atacctgata
     2581 gctgctgggc tgagtgatta ccaactaacg gaaaaagatt atcttatcct ggaacatttt
     2641 aaaaatattg aattttcagg ctttcctgtt actgagttat tctcagtgtg caatacagga
     2701 aacatattgt ctagagacat catagaaaat agtggggatg ttccttatct ttgtgctagc
     2761 cgtgagaata actctgttag ttcttatatc gcctatgata actcactctt ggaaaaaggg
     2821 aactgtattt ttattggtgg taagacattc gttgtaactt atcaggagcg agactttttt
     2881 tcaaacgaca gtcataattt gcgactctat gttaacaatg aggatggaag gacaaagttt
     2941 gcttatttgg gcattatttc atgtatt
//
LOCUS       DAZFKF010000250         2915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFKF010000250 DAZFKF010000000
VERSION     DAZFKF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.252"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(202..342)
                     /locus_tag="ACHKA1_005368"
     CDS             complement(202..342)
                     /locus_tag="ACHKA1_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5682877.1"
                     /translation="MSQKPLKTTVICITVVLIIWITHTSLCEFRFRIAGAEIAAFLQC
                     KQ"
     gene            complement(540..>2915)
                     /locus_tag="ACHKA1_005369"
     CDS             complement(540..>2915)
                     /locus_tag="ACHKA1_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5682878.1"
                     /translation="VVWPYTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGW
                     QTSTELVEDPDAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGS
                     QGLRHTPGDIIEICDNDYAGTLTGGRILSIDAASRTLTLDREVILPEAGTSTVNLING
                     SGKPVRVDITAHPAPDRIQVSVLPDGMETYGVWGLSLPSLRRRLFRCVSIRENTDGTF
                     AITAVQHVPEKEAIVDNGARFEPMSGSLNSVIPPAVQHLTVEVSASDGQYLALAKWDT
                     PRVVKGVRFSLRLTSGSGENSRLVTSAITADTEHRFSGLPPGEYTLTVRAINSYGQQG
                     EPATTTFRINAPAAPASIELTPGYYQITAVPHLTVYDPTVQFEFWFSEAKIADTSQVE
                     TSARYLGTGRQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLDFF
                     RGEIGKTHLAQGMWELIDNSQLDDEMAEMKTTITETRNEITQTVSKTLEDQSATIQQI
                     QRVQTDTNNDLAALYMLKVQKTKNGIPYVAGIGAGIEDADGQPLSNILLQADRIAMIN
                     PENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPEGRLTARNADISG
                     AVTANSGTLNNVTINQNCRILGKLSANQIEGDIVKTVGKAFPRDSRAPERWPSGTITV
                     RIYDDQPFDRQIVIPAVAFSGAKHEREHNDIYSSCRLMVKCNGRVLFERTALAESQVY
                     SGVIDMPAGRGHMTLEFSVSAWLVNGWYPTASISDLLVVVMKKSTAGISIS"
BASE COUNT          569 a          915 c          751 g          680 t
ORIGIN      
        1 catcccggta ccgcttttcc cgtaccatcc ttcagcacac ccgatattct gactgtcatg
       61 gatttccccc ataaaaaaac cgcagtaccg gtttcccggc ctgcggtaaa atttgtggtt
      121 tgttggtgtt aaaacggtgc catccggctg accaccctca gcaaccggtc ggcgggggat
      181 attctccccc gccacggttt cttactgctt acactgtaag aacgccgcaa tctccgcgcc
      241 cgctatccgg aaccggaact cgcacagtga agtgtgggtg atccagataa tgagcactac
      301 cgtgatacaa atcacggtgg tttttaacgg tttttgcgac ataaacgctt gctcctttta
      361 cggagaggcg ctaacctttc acttgcttag gatgaatggt caggcctcgg gttaaacatg
      421 aatgtttgtc cggggccttt tcacatccgg ccttcaggtg ttccctccgg ccatcagcca
      481 aaggcacccg cgcatactgt acggtttttg tctccttccg gcaatccggc tcctcatgat
      541 cacgaaatac tgatacccgc cgtggacttc ttcatcacga caaccagcag atcgctgata
      601 ctcgctgtcg ggtaccatcc attcaccagc caggcagaca cagaaaactc cagcgtcata
      661 tgcccccttc ctgcaggcat atcaatcacc cctgaataca cctgagactc agccagcgcc
      721 gtcctttcaa aaagcaccct gccgttacat ttcaccatca gccggcatga tgaataaata
      781 tcgttatgct ctctctcatg tttagcgccg ctgaaagcca ctgccgggat cactatttgc
      841 cggtcaaaag gctgatcgtc ataaatcctg acggtaatgg tccccgatgg ccaccgctcc
      901 ggtgcgcggg agtcccgcgg gaaagccttc cccacggttt taacaatatc gccttcaatc
      961 tggttggcag acagttttcc aagtatccgg cagttctggt taatggtcac attattgagc
     1021 gtgccgctat tcgctgtgac agctccactg atgtccgcat tgcgggccgt cagcctgccc
     1081 tccggcgtca gggagaacgt cgggggattg ccggacgagg tgatgctcac tgcaaacagt
     1141 cgcttcagga acacatcgtt catgaacagc tgattcccct gcgccacaaa cagcggcgtg
     1201 gtgttgccgt tctccgggtt aatcatcgcg atacggtccg cctgcagcag aatattgctc
     1261 aggggctggc catcagcatc ctcaatcccc gcacctatac cggcaacata cggaatgccg
     1321 tttttcgttt tctgcacctt cagcatgtac agcgcagcca ggtcgttatt tgtgtctgtc
     1381 tgcacccgct gtatctgctg tatggtggca ctctggtctt ccagggtttt actgaccgtc
     1441 tgcgtgattt cattgcgggt ttcggtgatg gtggtcttca tctccgccat ctcatcatca
     1501 agctggctgt tatcaatcag ctcccacatc ccctgtgcca gatgtgtctt cccgatttct
     1561 cctctgaaaa agtccagata cccttccgca tcattgctcg cccggccact ggcttccaca
     1621 aacgcagatt tccccaccag gttgacgctg cgcacataaa accagaaatc cttccccggc
     1681 ttaatgtgcg ggccggagac actccactgg cggcctgtcc ccagataacg ggcagaggtt
     1741 tccacctgag atgtgtctgc gatttttgcc tccgaaaacc agaactcaaa ttgtaccgtc
     1801 gggtcataca ccgtaagatg cgggaccgcc gttatctgat aataccccgg cgtcagttca
     1861 atgctggccg gtgctgccgg tgcgttaatc cggaacgtgg tggtggcagg ttcgccctgc
     1921 tggccgtagc tgtttatcgc cctgaccgtc agggtgtatt ccccgggcgg caggccactg
     1981 aaacggtgct ccgtgtccgc cgtgatggcg ctggtcacca ggcggctgtt ttcaccactg
     2041 ccactggtca ggcgcagact gaagcgcacg cccttcacca cccgcggcgt gtcccatttc
     2101 gccagtgcca gatactggcc gtctgaggca ctcacctcca ccgtaaggtg ctgcaccgcc
     2161 ggcgggatga cgctgttcag tgagccggac atcggctcaa agcgggcccc gttatccaca
     2221 atggcttctt tttccggcac atgctgcacc gccgtgatgg caaaggtacc gtccgtgttt
     2281 tcccggatgg aaacacagcg gaacaggcga cgacgcagtg acggcaggga gagtccccac
     2341 acaccgtatg tctccatgcc atccggcagg acgctgacct gtatccggtc aggggcgggg
     2401 tgtgcagtga tgtccacgcg caccggctta ccgctgccgt taatcaggtt caccgtcgat
     2461 gtccctgctt ccggcagtat cacctcacgg tccagcgtca gggtacggct ggcggcatcg
     2521 atggacagga tgcgtccgcc ggtcagggtc ccggcatagt cgttatcaca gatttcaatg
     2581 atgtcaccgg gcgtgtgccg cagcccctgt gacccgagcg tgaaatccac cgtctgcgtt
     2641 tccagcagtt ctgtctttat cacccacagt ccggcacggt gggcctgacc gcggctggta
     2701 cagccgaacg catccatctt cagcagattg cgcccgtagc gcaggatggc gtccgggtct
     2761 tccaccagtt ccgtggaagt ctgccagccg ttctgcgggt cggtgtaatt cacctccacc
     2821 gccgtgtgcc ggtccttcag ggcactgaag ctgtagcgga accccacgcc gttatcatcc
     2881 accaccacat cgctgttggt gtacggccac accac
//
LOCUS       DAZFKF010000251         2874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFKF010000251 DAZFKF010000000
VERSION     DAZFKF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.253"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            76..2355
                     /gene="ydeP"
                     /locus_tag="ACHKA1_005370"
     CDS             76..2355
                     /gene="ydeP"
                     /locus_tag="ACHKA1_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO5682879.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICSGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTQSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2603..2800
                     /gene="safA"
                     /locus_tag="ACHKA1_005371"
     CDS             2603..2800
                     /gene="safA"
                     /locus_tag="ACHKA1_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO5682880.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
BASE COUNT          789 a          656 c          694 g          735 t
ORIGIN      
        1 gataatccct ttgtatgtac tgtgcatcat cgctattaca aatcctaata attcatttcc
       61 acacaggata agtagatgaa gaaaaaaatt gaatcctacc agggagctgc cggtggttgg
      121 ggtgctgtta aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccaggatgtt
      181 attgccatgt ttgacatgaa taagccagag ggctttgact gtccgggttg tgcatggcca
      241 gatcctaagc acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg
      301 gaagtcacgg ataagcaggt aaacgcctct ttctttgctg agaatacggt tcaatcatta
      361 cttacctggg gagaccacga gcttgaggct gcggggcgac tcactcagcc tttgaaatat
      421 gatgccgtca gcgactgtta caagccatta agctggcaac aagctttcga tgaaattggc
      481 gcacgccttc aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact
      541 tccaatgagg ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt
      601 cccgactgct ccaacatgtg ccatgaaccg acaagcgtgg gtttggcagc gagtatcggt
      661 gtaggtaaag ggaccgtgtt gctggaagac tttgagaagt gcgatttagt catttgcatt
      721 gggcataacc ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa
      781 cggggagcga aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc
      841 gcaccgcaaa acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac
      901 tacaacgtgc gcattggtgg cgatatggcg ttgctcaagg ggatgatgcg cctgttaatt
      961 gagcgcgatg atgctgcaag tgccgcaggt cggccctcat tgctggatga cgaatttatt
     1021 caaacgcata ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa
     1081 gatatcgaac gtatttctgg actaagtcag acacaaatcg ccgaactggc tgatgcatat
     1141 gccgctgccg aacgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt
     1201 acccagaatg tacagcaact ggtcaatctg ctgttgatga aaggtaacat tggcaagcct
     1261 ggtgcgggta tctgcccact acgtggccac tctaatgtac agggcgaccg aaccgtcggt
     1321 atcaccgaga aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggcttcaca
     1381 ccacctcaag cacctggaca tgctgcaatt gccagcatgc aagcaatatg ttcagggcag
     1441 gctcgagcat tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg
     1501 agcgctgtac cgttaacgca attagatctg gcggtacacg tagccactaa gcttaaccgc
     1561 tctcatctgc tgaccgcacg gcatagctat attctgccgg ttctgggacg tagcgagatt
     1621 gacatgcaaa aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat
     1681 gcctcgcgtg gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc
     1741 gcgggaatcg cgcaggcagc actaccccag agcgtggtag cctgggagta tctggtggaa
     1801 gattatgatc gcattcgcaa tgacattgaa gctgtgctgc cagagttcgc cgattataac
     1861 cagcgcatcc gacatcctgg tggttttcac ctgataaacg ctgctgctga aaggcgctgg
     1921 atgactcagt caggtaaggc taatttcatt accagcaaag ggctgttaga agatccctct
     1981 tcagcgttta acagtaagct ggtcatggcg acagtacgca gccacgatca gtacaacacg
     2041 acgatttatg gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt
     2101 atgagtgcta aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt
     2161 gcgcttacgc cagacggtaa gcgcagctca cgccgcatgg atagattaaa agtggtcatt
     2221 taccctatgg ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaaca
     2281 cttgataacc acgatccatt aagtggcatt cctggctata aaagtattcc ggttgaatta
     2341 gaaccatcaa attaatgcct cttctcattt cttctgctgt catccgcaca gcagaagaat
     2401 acctcattga ctattatcgc gcaatttgct cacatggatt aaattaaact acatactata
     2461 agatataaac ttctgcctac agctgtaaga aactccgctc agtactgaag taccagtcct
     2521 atttcctctt ttctcaagcc tgttatatta agcatactga ttaacgattt tcaacgttat
     2581 ccgctaaata aacatatttg aaatgcatgc gaccacagtg aaaaacaaaa tcacgcaaag
     2641 agacaactat aaagaaatca tgtctgtaat tgtggttgtc ttattactga cccttacgtt
     2701 gatagccatt ttttcggcaa ttgatcagct gggtatttca gaaatgggtc gcattgcaag
     2761 agatcttaca catttcatta tcaatagttt gcaagactga aaataaacag cgaattataa
     2821 atatgaaatg ttaaaaaagt atcgataaaa ctttattgtt ttaaggagat aaaa
//
LOCUS       DAZFKF010000252         2867 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFKF010000252 DAZFKF010000000
VERSION     DAZFKF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.254"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..443)
                     /locus_tag="ACHKA1_005372"
     CDS             complement(<1..443)
                     /locus_tag="ACHKA1_005372"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO5682881.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRGSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECT"
     gene            complement(507..2168)
                     /locus_tag="ACHKA1_005373"
     CDS             complement(507..2168)
                     /locus_tag="ACHKA1_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO5682882.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPDPIYPDEAAHALAIFKQLRI
                     VDAPGSPTFGESCARWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQCKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGTHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEE
                     QGRCISLDDILRRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            complement(2165..2728)
                     /locus_tag="ACHKA1_005374"
     CDS             complement(2165..2728)
                     /locus_tag="ACHKA1_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5682883.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKTRRREAAAIPQPDENNPEMPPSAVMS
                     PGIEYMEDGLPDPVKAMGRILVENLCIDPKLALDAAWRLAQFTHHKKGDTGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
BASE COUNT          596 a          930 c          714 g          627 t
ORIGIN      
        1 gtgcattccg cattcgcggg cacggtgacg gcagacacct ccatcagttc ccagcgcaga
       61 aaatgcagtc cgcctccgtc cagaaaggtg tattcatggg gacggaagcc cacggacagc
      121 cccctgacca gcccggtctt aatggccgcc cagacctcat ccagccgggc agccagttgc
      181 gacggcatat ccggcacggg cttcaccagt gttgccgtga tttccagccc ttcgctgccc
      241 cggcgcaccg tacactgccc caccgggcgg gaatggtcat gctgccagag aaacgggatc
      301 gcactgccaa actccgcgcc ctccggctcc aggatgtcac catcccgatc cggagaaggc
      361 gttgacgcaa tcccggtgat cacccgttca tcctcactga aggatttcac cgtcagcagg
      421 gaacaggccc gtttaagagt cacatcagcc tcctgaaaat aaaaaaaccg ccggagcggt
      481 tcgtgatggt tacagtgtga acagggttat atgaaaaaaa ccgcatattc tttctttttc
      541 ggttccgggt taagggacat cagggagacc gcattgaaca gcgccatcag cgggtcaatt
      601 tttccccgtc cactggcctg tttggtgata agaatggcgt tacctttagg ctccacccgg
      661 gcattgccga cacaccaggc catcaggggc tggtcaccat gcaccagcac cccttcagcc
      721 agtttgcgct cggtggtttt aatggccccg cccagtttcc agccctggct tatccccacc
      781 acaattccgt cggggatccc ggcttccgcc agtgaatcca gaatctgccc cacccctgac
      841 gggtcaatac cgatatggtc cagtaactca gcctcatgaa tgcgacgcac atattccgcc
      901 acttccgccg tgtcatcccc gacacgccgg acaatggtca tatctccaca ggcaacaaga
      961 tcctgaaacc gggacgcctc gctcttccgt cggaccaccg cggtttcatg cgcccaggca
     1021 tggccccagc ccagccattc gcgggtctcc cggtcacgcc caatcacata catccccagc
     1081 agatcatcca gccctccgcc gtcaatcccc accgtcacca catcagcacg acgcaggata
     1141 tcgtccaggc tgatacaacg gccctgctct tcccagaaat cagcccccgc ccagcggtca
     1201 gagcgcaggg caagaccaat ttccacattg gcgtgttttg acatgaaccc ccggaatgtc
     1261 tcttcaccgg cttcccgggc tttacggtac tcccggtaca gaaaggcctc atccactgaa
     1321 tagccgagat tcggattgac catggcgagg ttttccatca gcaggtgagt cccgctttcc
     1381 accatttcag gagggtgttc aaatatcacc ggcagaaagt gcggatcatg aattttgccg
     1441 tcgcgcacat cccgggcgta ctgcagtttc tgtctgaaca ccccggcggg cggttcattc
     1501 gactgggtgg tcgtatacac cacaaaccct tccgggcggg aggcaaggcc gcctatggct
     1561 tcacgtaaca tgtcctccgc cttgcactgc ttgccaaaca gccacaactc atcaatcagc
     1621 gtacccacgg acttgatacc ggacaccgta ttcggatcgg ctgccaccac cttcagggtg
     1681 gtgtctgtca ccctgtgggt gatggtccgg atatgggtct gtacctggca gaggtcatcc
     1741 agatcatcgt cacgtcgtac catatcccgg gcagggttga aggcgttggc cgccacctcc
     1801 acagtcgggg ccagaatcgt gtaacccgcc gcctgccgcc agttcagtaa cagcgccgtc
     1861 atcatgatcc ccgcggccag cgtggacttc gagtttttct tggggataag gataaaaact
     1921 tccttgatat ggcgtacacc ggtctgcgca tcgtaggagc caaacagggc cgccaccagg
     1981 tcaaacaccc accgtgcaca ggactccccg aacgtcgggc taccaggtgc atccacaatc
     2041 cgcagttgtt taaaaatcgc cagggcatgt gcggcctcgt ccggataaat cggatccgga
     2101 ataatcgaca gccccttttt caggcgctct gcccagtccg ggcaggctgt gctccataca
     2161 ggtatcatcc gttgccctca ttatcgttat tcaccaccag tcggggtggc ggtggcaccg
     2221 caaaacggtt agccgctttt ttcgcagcat caccttttgc cgattttttc ccggtatccc
     2281 cttttttatg gtgcgtgaac tgcgccagac gccaggccgc atccagtgcc agtttcggat
     2341 caatgcagag gttttccacc aggatccgcc ccatggcttt caccggatcg ggaagaccat
     2401 cctccatata ttcaatacca ggagacatca ccgcggacgg tggcatctcc ggattgtttt
     2461 cgtccggctg tggtattgca gccgcctcac ggcgacgggt tttatcctcc tgctctgatt
     2521 ttttctgccg gtaaacagga acctcatcca cctccaccgt ctcgcattgt ttacgggcta
     2581 taaacgcaag cacctccgga tcttttgcca gctgcgagcc tttaaccctg gcggtcttcg
     2641 ccgaataacc ggcggcaatg gctgacgctg ttttgttttt cccggacatg agcgccagcg
     2701 caaattttcg tttttgcgtt gtcagcacag cctcctcccg ggtccagaac gcactcagcc
     2761 gggtatggtt cagcccattt ttcccggcgt ctcatgccgc aaatgttaac tgctgcctgg
     2821 ttaacatttg ctgaaaaagc cagttaacat tttttccgca caacaaa
//
LOCUS       DAZFKF010000253         2852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFKF010000253 DAZFKF010000000
VERSION     DAZFKF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.255"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..123
                     /locus_tag="ACHKA1_005375"
     CDS             <1..123
                     /locus_tag="ACHKA1_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5682884.1"
                     /translation="LSGGYRAVRDCWRRQIDKRAAEKISASQSAGSKPEEPITP"
     gene            149..394
                     /locus_tag="ACHKA1_005376"
     CDS             149..394
                     /locus_tag="ACHKA1_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5682885.1"
                     /translation="MSEVKPLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            472..687
                     /locus_tag="ACHKA1_005377"
     CDS             472..687
                     /locus_tag="ACHKA1_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5682886.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            691..1248
                     /locus_tag="ACHKA1_005378"
     CDS             691..1248
                     /locus_tag="ACHKA1_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682887.1"
                     /translation="MNHEEMNQRFSRLENEIAELNKKLSALMPSEDEKKRRDEQFAAF
                     DDYCRKVMSRNLAECFSIHNDNFSELEWECNRPSFVVSGDGVKITISENGKVTPPSHQ
                     HSEELIEFAIDYLKNNKKQGLMQRIGRCMGYLQVAAEIEALASGADKDSVVREALLRD
                     FDHPPFKKVPAYWLHPGLTYLKVRI"
     gene            1285..1818
                     /locus_tag="ACHKA1_005379"
     CDS             1285..1818
                     /locus_tag="ACHKA1_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5682888.1"
                     /translation="MNTKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GVGIWTICRGATRVDGNPVIPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFTSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            2337..2522
                     /locus_tag="ACHKA1_005380"
     CDS             2337..2522
                     /locus_tag="ACHKA1_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO5682889.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPVQAPCVKPPPPPAWIMQPA
                     PDWQTQLNGIISPSESG"
BASE COUNT          728 a          641 c          834 g          649 t
ORIGIN      
        1 ctttcgggtg ggtacagggc agtgcgcgat tgctggcgca ggcagattga taaaagggcc
       61 gctgagaaaa tcagcgccag tcagtcagcc ggaagcaaac ccgaagagcc cattactcct
      121 taacaacccc tttcaacgag aaaaccctat gtcagaagta aaacccctgg tcactgctga
      181 agcagtgaag gaagtcctgc gctctgaaga agtccggagc gcactgaaac agaaacttcg
      241 ccataacctg gaagcgcgtc ttgatgcaga agtggatgcc attctggatg aactgctggg
      301 cgcaccggca gctccggagc cggaaggcat cgcgggagag gggagtgctt cagatagcgg
      361 tgaccccaca cctgacagcg acatgatgat gtaagcatgc gtcagggacc atcggtgtgt
      421 gccggtggtc ttttttattg ttgtgagctt ccggattgcg ggagacgggg tatgtaccag
      481 atggaaaaaa tcacaacagg ggtgtcatac accacgtcag cggtgggaac gggctactgg
      541 ttcctgcagt tgctggacag ggtttccccg tctcagtggg cggcaatagg cgtgctgggg
      601 agtctgctgt ttgggctgct gacatatctg acgaacctgt atttcaaaat cagagaggac
      661 cggcgtaagg cggcacgggg agagtaggtg atgaaccatg aagaaatgaa tcagcgcttc
      721 agtcgtctgg aaaatgaaat tgctgaactg aataaaaaac tgtcggcgct gatgccttct
      781 gaagatgaaa aaaaacgccg cgatgagcag tttgctgcgt ttgacgatta ttgtcggaaa
      841 gtgatgagca gaaatctcgc agagtgtttc agtattcata atgataattt cagtgagctg
      901 gaatgggagt gtaaccggcc atcctttgtt gtatccggtg atggtgtgaa aataaccatc
      961 tcagaaaatg ggaaagtaac acctccatcg caccagcaca gtgaggagct cattgaattt
     1021 gccattgatt acctgaagaa caataaaaaa caggggctga tgcagcgcat tggtcgttgc
     1081 atggggtatc tgcaggtagc cgctgagatt gaagcgctgg ccagtggtgc ggacaaggat
     1141 tcagttgtgc gggaggcact tcttcgtgat tttgatcatc cgccctttaa aaaagtgccg
     1201 gcttactggc ttcatcccgg actgacttat ctgaaagtgc gtatttagtg ggcctgggac
     1261 agcggctgaa tatttaatat atccatgaac acaaaaatca aatacggcct gtcggctgcc
     1321 gttctggcgt tgattgccgc aggggcttct gcgcctgaaa tcctcgacca gtttctggat
     1381 gaaaaagaag gtaaccacac cacggcatac cgtgatggtg tgggtatctg gaccatctgc
     1441 cgtggagcca cccgggtgga tggtaatcct gtgattcctg gcatgaagct gtcgaaggaa
     1501 aaatgcgacc aggttaacgc cattgaacgg gataaggcgc tggcatgggt ggagaaaaac
     1561 atcagagtgc cactgactga accccagaaa gcgggtattg cgtcattctg tccttacaac
     1621 attggcccgg gtaagtgttt cacgtcgacg ttttataaac gaattaatgc aggagatcgc
     1681 aggggagcgt gtgaagcgat tcgctggtgg attaaggacg gtggcagaga ctgccgtatt
     1741 cgctcaaata actgttatgg tcaggtatcc cgtcgtgacc aggagagcgc gctggcgtgc
     1801 tggggaatcg acagataagc agaatatttt gctgaaaaat gacgttggtc aacgcggacg
     1861 gataacacga aatcctgcga actggcaaaa cctaagtgaa taaaagtaaa aaacccgttt
     1921 gttagcagca ggtggggttt tgtgtttcct gactccggaa aagtcaaagg agaaagtgtg
     1981 tttgatttta gcaaactgat tcgggagatt tgagtgatgg ctgaaaaatt atccacctgg
     2041 aagttcatcc ttatctggct ggtgtttgtg attatggcct ccggttattt catcggtcag
     2101 atacgctggt ggtgaaatga accgcgttct gtgtgtggtc atcattgccc tgctggtggc
     2161 ctgtggtgcg cttagtctgg ggctgaatca ttaccgcgat aacgccatca cctacaaaga
     2221 gcagcgcgat aaaaaagcca gagagctgga gctggcaaac gcaaccatta ctgatatgca
     2281 gcagcgccag cgcgatgttg ctgcgctcga tgcaaaatac tcgagggaat tagccgatgc
     2341 gagagctgaa aatgaaactc tgcgtgctga tgttgccgct ggtcgtaagc gcctgcggat
     2401 caacgccacc tgtccaggct ccgtgcgtga agcccccacc acctccggcg tggataatgc
     2461 aaccggcccc cgactggcag acacagttga acgggattat ttcaccctca gagagcggtt
     2521 gatgatgatg cagaagcagc tggaaggggc gcaggaatat atccgcactc agtgcctgaa
     2581 ataagttttg ctgatgcgcc gtatcgtcgc cgtattcccg tattaacaga gaccgcagcc
     2641 cgacagggag actcctctgc gagagtgtgc ggggataatc aaaaacgata cacaccgggg
     2701 tttaccgcgt taacggagcg cggcgttgtc ccctcatagt cgcctgtccg gtgcgatggt
     2761 ggaagaaact ggatttgttg caactgataa acattatcat tttctcgggt cctttccggc
     2821 atatggaccc gttacggggc ggcgacctcg cg
//
LOCUS       DAZFKF010000254         2822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFKF010000254 DAZFKF010000000
VERSION     DAZFKF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.256"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2697
                     /locus_tag="ACHKA1_005381"
     CDS             <1..2697
                     /locus_tag="ACHKA1_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5682890.1"
                     /translation="TGDGTVILAQKADAAGRVRAFSEVSIVSGRPVVVLQDSHQIEGD
                     RIRWGYRGGTLDINGNDMTFHRLAAADEGAVLTSRAGSATVRLDFSPSGQKAVMWHGH
                     FTGNLSVLNNTSSAVDFIMDGGADMSGSFTQQGGGLYIQGHPVVHAVSSETVAAALRK
                     QGDNSVLTQPVSFTQKDWESRTFSIGQLKLKEASFSLSRNATLTGDIDADNATMVLGS
                     DSLYLDMKDGTGSSSAPVKGTSAAGGASGTSTFRGNVNMRHSSLTVRDHFTGSITASD
                     SRIAVSSENVRLEGDSRLTSSALTVSDGGRLHVKGGLETDGGVTLDGGTLLVDGGSVR
                     NDVYERLLAWSEVRGGLNGSGEYDFMTGAAGLLRGYVRGSAGNVNLQNAAWMMTGNSS
                     VKHLESSGSALYFSRPGGEFHTLTAGSMDISDSVLVMRTDLHHSDQLRVTESLRGKNN
                     LLLVDFTERSDGQKALNIPLVTAPAGTGADVFSVKTRDTGFSHITPVVRAEQGTGGTA
                     WQLNVVQPETAAEPVVDEVSRPSLPVVMRQDAKMPNPVEAVSPLPSPVSAPSPAPEAS
                     VTDVPTGENTGESGEYRDETRWLLTGYRSTVNVSAVRDAGMLMSMGHRNFINEVNNLN
                     KRMGDLRDINGEAGAWARIMSGTGSAGGGFSDNYTHVQVGADKKHELDGLDLFTGVTM
                     TYTDSNAGSDTFSGKTKSVGAGLYASALFDSGAYIDLIGKYVHHDNEYTATFAGLGTK
                     DYGSHSWYAGAEVGYRYHVTEDAWIEPQAELVYGAVSGKQFSWKDQGMSLSMKDKDYN
                     PLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDGRM
                     LMSVGLNAEVRDNIRFGLEFEKSAFGKYNVDNAVNAGFRYSF"
BASE COUNT          620 a          644 c          936 g          622 t
ORIGIN      
        1 acgggggacg gaacggtcat tctggcacag aaggctgatg ctgcaggcag ggtccgggca
       61 ttcagtgaag tcagtattgt cagcggacgg ccggttgtgg tgctgcagga cagtcatcag
      121 attgaggggg accgcatccg ctggggatac cggggaggca cgcttgatat caacggcaat
      181 gatatgacct tccaccggct ggctgcggct gatgaggggg cagtactcac aagccgggcg
      241 ggttctgcca cggttcgtct ggatttcagc ccgtcagggc agaaggccgt gatgtggcat
      301 ggtcatttta ccggcaacct cagtgtcctg aacaacacgt catcagcggt ggatttcatc
      361 atggatggcg gagcggatat gtccggcagt tttacacagc agggcggggg actgtatatc
      421 caggggcatc cggttgttca tgccgtgagc agtgagacgg ttgcggctgc attgcgaaag
      481 cagggtgata attctgttct gacgcagccg gtatcattta cccagaagga ctgggagagc
      541 aggacgttca gcatcgggca actgaaactg aaggaggcgt cattcagcct ttcccgcaat
      601 gcgacactga cgggggatat tgatgctgat aatgccacca tggtgctggg aagtgactcg
      661 ctgtatctgg atatgaagga cggcacgggg agcagcagtg cgccggttaa ggggacgtct
      721 gctgcgggcg gtgcatccgg taccagtaca ttccgcggga atgtgaatat gcgtcattct
      781 tccctgacgg tccgcgacca ttttaccggc agtataacgg catcggacag ccggatagcc
      841 gtgagctcag agaatgtgcg tctggaaggg gattcccgcc tgacgtcttc tgcacttact
      901 gtcagtgacg gcgggcgttt acatgtgaag gggggactgg agacagatgg tggtgtgaca
      961 ctggatggag gaacccttct ggttgacggg ggaagtgtca ggaatgatgt gtatgaacgt
     1021 ctgctggcct ggtcagaggt gcgtggcggg ctgaacggaa gcggtgagta tgattttatg
     1081 acgggggcag ctggtctcct gcggggttat gtcagaggga gtgcaggtaa tgtgaatctg
     1141 cagaatgcgg cctggatgat gacgggaaac tcatccgtga agcatcttga gtcatcggga
     1201 agtgctctgt atttcagcag gccgggcggg gagttccata ccctgacggc aggcagtatg
     1261 gatatcagcg acagtgttct ggtgatgcgg acggaccttc accactcaga ccagcttcgg
     1321 gtgacagaat cgctgcgggg taagaataat ctgttgttgg ttgattttac tgagcgttct
     1381 gacgggcaga aggcgctgaa tatccctctg gtgacagcgc cggccggtac cggcgcggat
     1441 gtgttcagtg tgaaaacgcg tgataccggc ttcagtcata tcacacctgt tgtcagggct
     1501 gaacagggga cgggcggcac ggcctggcag ctgaatgttg tgcagccgga aactgccgca
     1561 gagcctgtgg tggatgaggt gagcaggccg tcgttgccgg tggtgatgcg gcaggatgca
     1621 aagatgccga atcctgtgga agccgtatca ccgctccctt caccggtttc tgctccgtca
     1681 ccggctccgg aggcgtcagt gacggatgta ccgaccggag agaatacggg ggaatcaggt
     1741 gagtaccgtg atgagacccg gtggctgctg acaggatacc ggagtacggt taatgtctct
     1801 gcggtcaggg atgccgggat gctgatgtca atggggcacc gtaacttcat taatgaggtc
     1861 aacaacctga acaaacgtat gggtgacctg cgggacatca acggcgaggc cggtgcatgg
     1921 gcccgtatca tgagcggaac cggctctgcc ggtggtggct tcagtgacaa ctacacacac
     1981 gttcaggtgg gtgcggataa aaaacacgaa ctggacggtc tggacctctt caccggggtg
     2041 accatgacat ataccgacag caacgccggc agcgacacct tcagtggtaa aacgaagtca
     2101 gtgggggcgg gtctgtatgc ttcagccctg tttgattccg gggcctatat cgacctgatt
     2161 ggtaagtatg ttcaccatga taatgagtat acggcgacct ttgccggact tggtacaaaa
     2221 gactacggct ctcattcctg gtatgccggt gctgaagtgg gttaccgcta tcatgtcaca
     2281 gaggatgcct ggattgagcc acaggcggaa cttgtttacg gtgctgtctc cgggaaacag
     2341 ttcagctgga aggaccaggg gatgagcctg tccatgaagg acaaggacta caatccgctg
     2401 attggccgta ccggtgtgga tgtgggtaaa tcgttctccg gtaaggactg gaaagtgacg
     2461 gcacgtgccg gtcttggcta ccagtttgac ctgctggcga acggagaaac ggtactgcgt
     2521 gatgcatccg gagagaagcg tatcaagggc gagaaagacg gccgtatgct gatgtctgtg
     2581 ggtctgaacg cagaggtccg tgacaacatc cgttttggtc tggagtttga gaaatcagcc
     2641 tttggtaagt acaacgttga taatgctgtc aacgccgggt tccgttattc attctgacgg
     2701 ttgttactga aggggattat ggcctgacgg ctccctgtaa ataaagacag gcacagaaga
     2761 ttgtgagtta ctcagtgttc caccaaaaag taaggtcgca actctgtgcc tgcttcgtaa
     2821 gt
//
LOCUS       DAZFKF010000255         2801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFKF010000255 DAZFKF010000000
VERSION     DAZFKF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.257"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            176..1570
                     /locus_tag="ACHKA1_005382"
     CDS             176..1570
                     /locus_tag="ACHKA1_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO5682891.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAEGQVKQLQTQQAAEIREIKAAQGNTPVNGQTTAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            complement(1611..1925)
                     /gene="rhaM"
                     /locus_tag="ACHKA1_005383"
     CDS             complement(1611..1925)
                     /gene="rhaM"
                     /locus_tag="ACHKA1_005383"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO5682892.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(1935..2759)
                     /gene="rhaD"
                     /locus_tag="ACHKA1_005384"
     CDS             complement(1935..2759)
                     /gene="rhaD"
                     /locus_tag="ACHKA1_005384"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO5682893.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAHEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAEVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
BASE COUNT          712 a          637 c          731 g          721 t
ORIGIN      
        1 ttatattcct cacgttccag gaaatcagcg gatttactca tattatgcgg cgggatacta
       61 tccatcggcc tgctgaagtt ggattaaaaa ttcgaaagtg cacactttat atttctctat
      121 atacgtattc cttttggaaa gcgtagggcc tcctttattt cagggaacgt aaataatgaa
      181 aaaatcgaca ttatctttag ccatcggttt attattggca tgtagtaccg gtatggcaaa
      241 aacacagcat ttaacgctgg aacaacggat ggcattgctg gaagaacgcc tggaagcggc
      301 agaaatgcgg gcagcaaaag cagaagggca ggtaaaacag ctgcagacac aacaagccgc
      361 tgagatccgc gaaattaagg ccgcccaggg caatacgccg gtaaatggac agacaacggc
      421 ggaatctgca aagaaaaatg ccacctcacc taatcttttg ctttcaggtt atggcgattt
      481 aaaaatctac ggcgacgtag aatttaatat ggatgcggaa agtaatcatg gcctgctggc
      541 aatgaccaac gctgatgtga atagcgatcc cactaatgaa cagtggaatc tcaatggtcg
      601 tattctgtta ggttttgacg gtatgcgaaa gatggacaac ggttatttcg ccgggttctc
      661 cgcacaaccg ctgggtgata tgcacggttc agtaaatatc gatgatgcgg tattcttctt
      721 tggcaaagaa aacgactgga aggtcaaagt aggccgtttt gaagcctacg atatgttccc
      781 gctgaatcag gatacctttg ttgaacattc cggtaatact gcgaacgatc tttatgacga
      841 tggcagcggt tatatctata tgatgaaaga gggccgcggg cgctctaacg ctggcggtaa
      901 tttcctcgtc agcaaacaac tcgataactg gtattttgaa ttaaacacgt tacttgaaga
      961 cggaacatct ttatataatg acggtaatta tcatggacgc gatatggagc agcagaaaaa
     1021 tgttgcttat ctgcgcccag taattgcctg gtcgccgacg gaagaattca ccgtttccgc
     1081 agcgatggaa gcgaacgtgg taaataatgc ttatggttat accgatagca agggtaattt
     1141 tgtcgatcag tccgatcgta ccggctatgg tatgagcatg acctggaacg gcctgaaaac
     1201 ggatccggac aatggcattg tggttaatct taataccgcc tatttagatg ctaataatga
     1261 gaaagatttc actgccggga ttaacgcgct gtggaaacgt ttcgagctcg gttatatcta
     1321 cgcgcacaat aagattgatg aattcagcgg tgtagtttgt gataacgact gctggattga
     1381 tgatgaaggg acgtacacca ttcacaccat tcatgcgtct tatcagttcg ctaatgtgat
     1441 ggatatggag aactttaata tttacctcgg gacgtattac tccattctgg atagcgacgg
     1501 cgataaaaaa cacggtgatg atactgatga ccgttacggc gcacgcgttc gctttaaata
     1561 cttcttctga tgaagtcgcg gtgctggata ttttccggca ccgcaactca ttaaggcagg
     1621 taaaacactt cttgcagctc gctactcacc gggctgttat ccgggttagc gggcataacg
     1681 tcggtcatat atttccacca acgttggcag atttcagtgc tggctaccgc attccaccgt
     1741 tcttcagatt caatctctac catggcaaac agcagattac gcgctttgtc gaggtaaatg
     1801 gcgtagttat gcgcaccgtg agatttcaac actgcttcca gttccggcca gatggggtta
     1861 tgccgacgct gatactcttc gtgggcgtcg gggttaacct gcatgacaaa ggctttgcgg
     1921 atcatgtttg ctccttacag cgccagcgca ctggctagtg gtgtaacgcc gaagcgcttg
     1981 ccgagcgcta tcaactcttc acggctgatg gtctgtttca tgccgcccat cgaatacacc
     2041 ttcactaaca cttccgctga tttttctgcg gtgtcgatta aaccgaatgt ttcatccagc
     2101 gtcggtccgg aaccgaacac accgtggaag ggccacaaca ccagcgaatg tttttgcatc
     2161 tcgtgggcag ttgcctggcc gatttcgtcc gtgccgggca ccatccacgg cagaatgcca
     2221 acgccatccg ggaataccac cagacactcg gtgctgcctt cccacagttg gcgagtgaag
     2281 accgcggtgt cgttttccag tacataggtg agggcgatca ggttggtggc atggcagtgc
     2341 atgatcaccc gatctttacc gttggtggct ttaatacgct cgcagtggga gagaaagtga
     2401 gccggaagtt cagaagttgg aacggcttcg ttggttaacc cccagagaat atggtagccc
     2461 gcgccgtcgc tgtcgacttt tacgacgcct aagttagctg caggatcaag ctggacgtta
     2521 cggaagaatt tgccggagcc ggtgacaata aacggcgtat ttgccagtaa aggcatgggc
     2581 tggctgagcg gaatatagcg cggttgtggg tggaagttgt cgtgataaag cgcgatatcg
     2641 gcgtcatcca ggcgcagcgt caggttgccg ccgttgcgct cgtcccagcc ttttagccag
     2701 gcgtcggtgg tggctttgat cattccctgg acaaaccagg actgggtaat attttgcatg
     2761 ttctgtgttc ctgtaaattc ggtgttgtcg gatgcacgac c
//
LOCUS       DAZFKF010000256         2777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFKF010000256 DAZFKF010000000
VERSION     DAZFKF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.258"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            130..822
                     /locus_tag="ACHKA1_005385"
     CDS             130..822
                     /locus_tag="ACHKA1_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO5682894.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            845..2272
                     /gene="mdtD"
                     /locus_tag="ACHKA1_005386"
     CDS             845..2272
                     /gene="mdtD"
                     /locus_tag="ACHKA1_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO5682895.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(2238..>2777)
                     /locus_tag="ACHKA1_005387"
     CDS             complement(2238..>2777)
                     /locus_tag="ACHKA1_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO5682896.1"
                     /translation="GDSDLIQDNSLLGGDLATQYLIDKGHTRIACITGPLDKTPARLR
                     LEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGV
                     YQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELAIDVLIHRITQP
                     TLQQQRLQLTPILMERGSA"
BASE COUNT          668 a          683 c          689 g          737 t
ORIGIN      
        1 gaccgcgcat tttttcagca aaagccccgt taatgtgcca taaaacaagc aatttgccgt
       61 ttttgcaacc gtaatcacac ttcctggtgg catactaagg aggaataaaa agaaaaagga
      121 agattagtaa tgcctttaag cgcacaacag ttagctgcac agaaaaacct ttcgtatgtt
      181 ctggctgaga agctggcgca acggatctta aaaggtgaat atgaacccgg caccattttg
      241 cccggtgaga ttgagctggg cgagcaattt ggagtgagtc gtacagcggt acgcgaagcg
      301 gtcaaaacgt taacggcaaa agggatggtt ttaccgcgac cgcgaattgg tacccgggta
      361 atgccacaat cgaactggaa ttttcttgat caggaattgc ttacctggtg gatgacagaa
      421 gagaactttc atcaggtcat cgatcacttt ctggttatgc gtatctgtct ggagccgcaa
      481 gcctgcctac tggcagcaac ggttggcacc gcagaacaga aagcgcatct caatacgtta
      541 atggccgaaa tggcggcatt aaaagagaac tttcgccgtg aacgctggat cgaagtcgat
      601 atggcctggc atgagcatat ctatgaaatg agcgccaatc cgtttttgac ctcattcgcc
      661 tcgctattcc attcggttta tcacacttac ttcacgtcaa ttaccagcga cacagtgata
      721 aagctggatc tgcatcaggc gattgtcgat gcgattatcc aaagcgatgg cgacgcggca
      781 tttaaagctt gtcaggcgct gctacgctca cctgataagt gataacccga taacaggaca
      841 ttgaatgagc gataaaaaga agcgcagtat ggcgggtttg ccgtggatcg cggcgatggc
      901 cttcttcatg caggcacttg atgccactat tctgaatacc gccttacccg caatcgctca
      961 tagccttaat cgttctcctc tcgcgatgca atcagccatc atcagttata cgctgacggt
     1021 ggcgatgctt attccggtaa gcggatggct agccgatcgc ttcggtacgc gtcgcatttt
     1081 tacccttgcc gtgagtctgt tcacgttggg ttctctggcc tgcgcacttt ccaactcgct
     1141 accacagctg gttgtcttcc gggttattca ggggataggc ggcgcaatga tgatgcctgt
     1201 tgctcggctg gccttactgc gcgcttatcc tcgtaatgaa cttcttccag tattgaattt
     1261 tgtcgccatg ccgggtctgg tggggccaat tttaggcccc gttcttggcg gcgtgctggt
     1321 cacctgggca acctggcact ggatattttt aatcaatatc cccataggta ttgcgggcct
     1381 tctttacgcg cgcaaacata tgcccaattt caccaccgca cgacgcagat tcgatatcac
     1441 tggctttttg ctgtttggcc tcagccttgt tctcttctca agcggaatag agctattcgg
     1501 ggaaaagatt gtcgccagct ggattgcctt gacggtaatt gtcaccagca tcgggttact
     1561 gcttctctat attctccatg cacgacgcac gccaaaccca ttaatttcat tagatttatt
     1621 taaaacccgc actttctcga tcggtatcgt aggcaatatt gcaacccgtc tggggaccgg
     1681 ctgtgtaccg ttccttatgc cattgatgtt acaggtagga tttggttatc aggcgtttat
     1741 tgccggctgt atgatggcac cgacagcgtt aggttccatt attgcaaaat cgatggttac
     1801 ccaagtctta cgtcgtctgg gctatcgcca tacattagtg gggatcacgg tgattattgg
     1861 gctaatgatc gctcagttct ctttgcaatc accggcaatg gcgatatgga tgctgatctt
     1921 gccgttgttt atattaggga tggctatgtc gacgcagttt accgcgatga ataccatcac
     1981 acttgccgat ctgaccgatg acaacgccag cagcggtaac agtgttctgg cggtcacgca
     2041 gcaactgtct atcagtttag gcgttgctgt aagtgcggcc gtccttcgcg tttatgaagg
     2101 gatggaaggc acaacgactg tcgaacaatt ccactatacg tttatcacga tgggcattat
     2161 tactgttgct tcagcagcaa tgttcatgct tctgaaaaca accgatggta ataatttgat
     2221 caaaagacag cgtaaatcta agccgaaccg cgttccatca gaatcggagt aagttgtaat
     2281 cgttgttgct gaagggtcgg ctgggttatc cgatggatga gtacatcaat cgccagctcc
     2341 cccagttcat cttttggttg atggatagtg gttaatggtg gcgtcataaa gcttgccagt
     2401 tcgatatcgt catagccaat caccgcgata tcctgcggaa cctgtaactc tgcctgatat
     2461 aacgcctggt aaacgccaac agccatagcg tcatttccgg taaagacggc ctgaggacgc
     2521 agcggatgtg atagcagttg gcgcatagcg tcaaacccgc cgttaaattc aaaatcacca
     2581 gtgacttcat agccatcagg aatgttgaga cccgcacgtt tcatcgccgc ccgataacct
     2641 tccaaccgca ggcgcgccgg agttttatcc agcgggccgg taatacaggc gatacgggta
     2701 tgacctttat cgatcagata ttgcgttgct aagtctccgc ccagcaacga gttatcctga
     2761 ataagatcgc tgtcgcc
//
LOCUS       DAZFKF010000257         2740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFKF010000257 DAZFKF010000000
VERSION     DAZFKF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.259"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            165..653
                     /gene="eco"
                     /locus_tag="ACHKA1_005388"
     CDS             165..653
                     /gene="eco"
                     /locus_tag="ACHKA1_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO5682897.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(1029..2675)
                     /gene="mqo"
                     /locus_tag="ACHKA1_005389"
     CDS             complement(1029..2675)
                     /gene="mqo"
                     /locus_tag="ACHKA1_005389"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO5682898.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          644 a          757 c          687 g          652 t
ORIGIN      
        1 tctgctggtt tgttctctca tcagaattta acactaaaag agcaggtaaa attgtctgaa
       61 tattctttaa gttattcata aagcaaatta ataaatctga tgaatatgtt aaccttcagc
      121 gacatcatcg gtgcaaacct ataaatgaag aaggaaagca aaaaatgaag accattctac
      181 ctgcagtatt gtttgccgct ttcgccacca cttccgcctg ggcggcagag agcgtccagc
      241 cactggaaaa aatcgcgcct tatccacaag ctgaaaaagg catgaagcgt caggtgattc
      301 agttaacccc gcaagaagat gaatctaccc tgaaagtaga actgttaatc ggtcagacgc
      361 tggaagtcga ttgcaatttg catcgtctcg gcgggaagct ggaaagcaaa acgctggaag
      421 gctggggcta tgattattat gtctttgata aagttagctc gccggtttcc acgatgatgg
      481 cctgcccgga tggcaagaaa gagaagaaat ttgtcaccgc gtatctgggc gatgctggaa
      541 tgctgcgtta caacagcaag ctgccgatcg tggtgtatac gccagacaat gtagatgtga
      601 agtaccgcgt ctggaaggcg gaagagaaaa ttgacaacgc ggtagttcgc taaactgccg
      661 tgaagtgcgg caccccatag gttggataag gcggttacgc cgcatccaac atctaacgcc
      721 cgagccggtt gcctgatgcg acgctgacgc gtgttatcag gcctacaccg ctgtgaagtg
      781 ctccaccccg taggtcggat aaggcgggaa cgccgcatcc gacatccaac gtccgagccg
      841 gttgcctgat gcgacgctga cgcgtcttat caggcctaca ccgctgtgaa gtgctccacc
      901 ccgtaggtcg gataaggcgg ttacgccgca tccaacatct aacgcccgag ccggttgcct
      961 gatgcgacgc tggcgcgtct tatcagcata cgccacatcc ggcataccat gccggatgtg
     1021 gcacatcatt acaacgcaat atccgccact tctttttgaa cgggttgcgg tttcaactgc
     1081 ggtttcggcg tactatctgc tgcttgcggt ttgtcgtatt tcagccccag cacttcgctg
     1141 gtgtactgca actcgcgctc tgttgcggct acatcaccgt tcagcttgcg tccataagac
     1201 ggaacgatcg ctttcaacgt agcctgccat tgcgggctgg aaacgcgatc gccaaatact
     1261 ttttccagca gatccaacat aatcggcgcg gcggttgacg cccctggcga tgcccccagg
     1321 agcgcggcaa tggtcccttg ctggtcactg accacttcag tacccagacg cagtacacca
     1381 cctttatcgg catcacgctt gataatctgc acacgctgcc ccgcttgcca caaacgccag
     1441 tcctcttttt tcgcttgcgg atagtactct ttcaacgctt caaaacgatc ctcttcactc
     1501 aacatcacct gactcaccag atatttcacc agatcgaaat tatccagccc gacgtgcatc
     1561 atcggcatca cgttagaggt ggtggtggaa ctcattagat cccacaatga accgtttttg
     1621 aggaatttgg ttgagaaggt ggcaaatggc ccaaacagca ctacgcgttt accgtccaga
     1681 acgcgggtat cgatatgcgg aaccgacatc ggtggtgcgc caacggatgc tttaccgtaa
     1741 accttcgcca gatggtgatt aaccacgtcc gggttttccg aaacaaggaa ctgaccgccc
     1801 accgggaaac cggcgtagtc tttcgcttcc ggaatccccg attcctgtaa cagcttcagt
     1861 gccgcaccgc ccgcgccgat aaagacaaat ttggcacgga tgttctgtgc agtgccattt
     1921 ttcagatcgg caacggtaac ggtccaggtg ttatcgtcat tacgctttag ggcgcggact
     1981 tcgctgctga gttgcagcga gaagttagat ttcttctgca aggaagcaat taactggcgg
     2041 gtgatctcgc cgtagttcac atcggtacca atttccgtac gcgtggctgc cactttctgt
     2101 tgcggatcgc gcccttccat cactaacggt gcccactctt tgatctgcgc gtgatcttca
     2161 gagtaacgca taccgcgaaa tagcgagctt tgttgcaacg cggcgtaacg ggcgcgcagg
     2221 aaattgacgt tatcctcgcc ccagacaaag ctcatatgcg gaacggtatt gataaatgaa
     2281 cgcggagtac gcagcacgcc gcgctcgacc tggtgcgccc agaactggcg ggaaatctga
     2341 aatgcttcgt taatggcgac tgctttttca atactgatgc tgccatcggc gttttgcggg
     2401 gtgtagttca gttccatcag tgcagaatgc ccggttccgg cgttgttcca gccgttcgaa
     2461 ctctcctgcg cgaccccctc caggcgctcc accatggtca tcgaccattc aggctccagc
     2521 tcgcgtaaat aggtccccaa cgtggcgctc ataatgccgc cgccaatcaa cagtacatca
     2581 gtttcctgct cctcggacgc tttcgctttt gccgccatcg aaacggcatt aagccccacg
     2641 gccatcgaga agagcatggc agtcactttt ttcatcttgt taatgcctta cttttagtcg
     2701 ctttattgca ggtgagattt gcgcggcatc aacggtaaca
//
LOCUS       DAZFKF010000258         2719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFKF010000258 DAZFKF010000000
VERSION     DAZFKF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.260"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            33..107
                     /locus_tag="ACHKA1_005390"
     CDS             33..107
                     /locus_tag="ACHKA1_005390"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682899.1"
                     /translation="MSQQVQRLAGQVDRLSRLFITNSR"
     gene            complement(715..1014)
                     /locus_tag="ACHKA1_005391"
     CDS             complement(715..1014)
                     /locus_tag="ACHKA1_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin"
                     /protein_id="HFO5682900.1"
                     /translation="MREISQRDLYQVLGAGNTDPNTQLLKDLANNMAWGAALGAKGGL
                     GTAAVGAGGAAVQTVVQGLINHGPVSVPVPVLIGPSWNGSGSGYNSATSSANGGS"
     gene            complement(1605..1749)
                     /locus_tag="ACHKA1_005392"
                     /pseudo
     CDS             complement(1605..1749)
                     /locus_tag="ACHKA1_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314450.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            2122..2310
                     /locus_tag="ACHKA1_005393"
     CDS             2122..2310
                     /locus_tag="ACHKA1_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682901.1"
                     /translation="MDNRVSVFEKGESARRSRTTERSESVSEEAEKRLDEHFLLTHLC
                     GISHPAWCTFHTIRTVAA"
     gene            complement(2462..2653)
                     /locus_tag="ACHKA1_005394"
     CDS             complement(2462..2653)
                     /locus_tag="ACHKA1_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO5682902.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
BASE COUNT          648 a          637 c          731 g          703 t
ORIGIN      
        1 agaacgggca actgagggaa cagtgcgacg acttgagtca gcaggtgcag agattagccg
       61 ggcaggtgga ccgcttgagt cggttattta tcacgaacag cagataaggg atgaacagga
      121 aaggcaggca cgcctggaac gggagcgtgc gcggtcctgg catgaagagc aggaacggga
      181 agctcgggta tggcgtggcc cgtcaatggg gatgtgacgg tgagttgttc cgggaggttg
      241 ccagatggta ccggaacgcg ttaaaaacgc atccgggcgc gattctggcg tgtcgttttt
      301 gttgctgtgt tttgcctctt tgccagatcg cgctcattcc tcagcctcat cccgagatgt
      361 ctgcgtatgt catcaactga aaagatggaa aaaaaacagt gtcatgaggt ttacgcaggc
      421 aaaaccagcg ttattcacat ggctgaatag ctatgcatac ggttaaaatt tatcaggtgc
      481 gatcgcggca gcttttcggg tggtttgttg ccatttttac ctgtcagcag ccgtgatcgc
      541 gctgaacgca tttcagcggt gcgtacaata agggattatg gtaaatccct tccctgggtt
      601 ccgtcccttc ttccggtttt aagctgcaat ttctttgctg ttaggttaac agcgctctta
      661 cgtaccagac cgctttgttg tcaaaggaat tacggtttgg agctccaaat aagattaact
      721 gccaccattg gcgctagatg ttgcgctgtt ataaccacta ccactaccat tccagcttgg
      781 tccaatgagt acggggacag ggacacttac aggtccatgg ttaattagtc cttgtactac
      841 tgtttgtacc gctgctccac cagcacccac agctgccgtg ccaagccctc cttttgcacc
      901 taaagctgca ccccatgcca tgttattagc aagatctttt agcagttgag tatttgggtc
      961 ggtgttgcct gcgccaagaa cctgatataa gtctctttga ctaatctctc tcattcttta
     1021 ctcctataac tgtaagtatt cctatgttta ttgcaattgc tactaatgaa gacaaaaaac
     1081 aaaagaggta gaaattgtca aatatattgt aacttccagt taagtacacc tttgttaatg
     1141 gatataaaaa acaaatgaaa aaacacatta atatcaacac gataactctg ttgtgtgttt
     1201 tttggggata tgagcggctg cgcaaaataa tacagaacgc catgactgac aagaaaacag
     1261 gggaaaatgc aaatgaaagt aatgtcatgc gtattatcct taatatccaa aacagatgca
     1321 ttagtatgat tattaaccga atttttgttc ttatctatcg cttttttagg agggggagga
     1381 cggggctatt tggaaaaaga tcaaaggagc tcttgagatc ctttttttct atgcgtaatc
     1441 tgctgcctgt aaacgaaaaa acccgccttg tggcgggttc ttccgtccga tcagaagtct
     1501 tcgacctctt ctaatcggcg gtggtaactg gctttagtgg agcacaggta cctaaatggt
     1561 ccttctagtg tagtcaaaat cagactgata cttcaagaac tctgttacca acttataaat
     1621 ctcgctctgc taacccggtt accaatggct gctgccagtg gcgttaagtc gtgtcttttc
     1681 cgggttggac ctaagagaac agttaccgga gaaggcgcag cagtcgggct gaacgggggg
     1741 ttcctgcata cagcccggcc cggagcgaac tgcctaagtc gccccgagat accagaagac
     1801 gtgagctatg agaaagcgcc acgtgtcccg caagggagaa aggcggaaag gtatccggta
     1861 atcgatatgg cgggaacgtg ggggcgcagg agggagcgat tcaccggaaa cggtgatgat
     1921 ctttaagcca cgtcgggttt cgccaccgct ggcggatttc atggttgagc cgtaaggctc
     1981 ccacagatgc accgaaaaag cgtctgtttt gtgaagtccg tcaggggggc ggagcctatg
     2041 gaaaaagcgc ccccggcgcg gccttttccg gggttttcgt tttttgcgca catgttcttt
     2101 cttggtttat ccccgggttc tgtggataac agggttagcg tttttgagaa aggagagtcc
     2161 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaaaagcgc
     2221 ctggacgagc attttctcct tacgcatctg tgcggcattt cacacccggc atggtgcact
     2281 tttcatacaa tccgcactgt tgccgcatga ttaagccagt atacactccg ctatcgctac
     2341 gtgactgggt catggctgcg ccccgacacc cgctaaaacc cgctgatgcg ccctgacggg
     2401 cttgtctgct cccggcatcc gctcacaggc cagctgtgac cgtctccggg agctgcatgt
     2461 gtcagaggtt ttcaccgtca tccccgaagc gtgcgaggca gctgcggtaa agctcgtcgg
     2521 cgtggtcgtg aagcgcttcg cagatgtcag cctggtcgtc ggcgtcaagt tcattcagtt
     2581 tttccagcag ggtcagcgtc tggcttctga tgaagcgggc catgttaagg gcggtttttt
     2641 cctgtttagt cacagttctg tttctccatg tgtggggagt ctgtcggagg tttttaatga
     2701 taccggtgaa acgcgaaag
//
LOCUS       DAZFKF010000259         2689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFKF010000259 DAZFKF010000000
VERSION     DAZFKF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.261"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..875
                     /locus_tag="ACHKA1_005395"
     CDS             <1..875
                     /locus_tag="ACHKA1_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO5682903.1"
                     /translation="YWMMKASLPASMNKVITDVGPGKLNLGGCVIEVMGTYEAAIVRV
                     TIGEYGATGFINGTVCTCTVYGDTQYFHWRVDYSTKNKPDTVSQRDASTTQKGVVQLS
                     SDTGSNDETKAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGEKGDPGAQGPKGE
                     TGATGPAGPRGAQGPKGEKGDPGPRGERGETGPMGPPGASGDDGKSRVMGFRLGNPVT
                     YELQEGDHGWSVNLADGSVITGLSGNTQGGVSRVTTIYYRPLQMTFDGSHWRTVGVGE
                     YVSSGAPGREYFSL"
     gene            917..1588
                     /locus_tag="ACHKA1_005396"
     CDS             917..1588
                     /locus_tag="ACHKA1_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO5682904.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGIGRVASVLWPVGQSVVEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNEMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     GWAQA"
     gene            1664..1846
                     /locus_tag="ACHKA1_005397"
     CDS             1664..1846
                     /locus_tag="ACHKA1_005397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682905.1"
                     /translation="MTMLVYGVKTATEMLILYVMLILFPEHPVDVNNFQIFGSESLDI
                     SIYATVDVFCGAWQCD"
BASE COUNT          732 a          532 c          810 g          615 t
ORIGIN      
        1 cttactggat gatgaaggca tcactgcctg cgtcaatgaa taaagtcatc acggatgtcg
       61 gaccgggaaa actcaatctg ggtggctgtg tcattgaggt gatgggaacg tatgaggctg
      121 ccatagtccg ggtcaccatt ggcgaatacg gtgcaacggg gtttattaat ggcacggtct
      181 gtacctgtac ggtttacggc gacacacagt atttccactg gcgggtggat tacagcacaa
      241 aaaacaaacc ggatacggtc agccagcggg atgccagcac gacgcagaaa ggtgtggtgc
      301 agttaagcag tgatactggc agtaatgacg aaacaaaggc agccacgccg aaggccgtga
      361 aggcggcaat ggatgtggca aatgaagcga aaacaaaggc agaagaggct gcagcaggag
      421 gtggtgttcc cggtccgaaa ggagagaaag gtgaccccgg agcacagggt ccgaaaggtg
      481 aaacgggagc aacaggcccc gctggtccgc ggggagcgca gggaccgaaa ggagagaaag
      541 gagaccccgg tcccagaggt gagcgtggag aaaccgggcc gatggggccg ccgggggcat
      601 caggagatga cgggaagagt cgtgtcatgg gcttcaggct ggggaatccg gtaacgtatg
      661 agttgcagga gggggaccat ggctggtcag ttaatctggc tgatggttca gtcattacag
      721 ggctttcggg gaacactcag ggcggcgtca gccgtgttac aacgatttac tacaggccgc
      781 tgcagatgac gtttgatggc tctcactggc gcaccgttgg tgttggtgag tatgtgtcgt
      841 caggagcgcc ggggcgtgag tatttttcat tataaagcgc atcatctttc agacatgtaa
      901 ccgggaggac ttacagatgc acataaagaa tttcagacaa tacacgccgg aaaatccgga
      961 tgtgccgggt gcgatgtacc tgaaatcaga ggatggtcag gactggtatg agtgtcagtc
     1021 gttattttca gcagagacgc tgaaggtggt ttataacagt gccggagtca ttaccggcat
     1081 cggcagggtg gcgtcagttc tgtggccagt gggccagagt gtggtggagg tggcggatac
     1141 ggaagaaaac cgcaaagctg acatctcggg gcgctggggt tttgacgggg agaaaatcac
     1201 ggacctgctg accgcggaga aagcgcgcgg gatgaagggc gatgaaatta acgcctggcg
     1261 taatgagatg gaagcggcga actacacgtt tgagcacaat gggcgtaaat gggactacgg
     1321 gaagtcaacg cagacgcgtc ttgagccgtc ggtggcggca gcgaaagcgg ggaaactgcc
     1381 ggaggcgttt ttctggacgg atgcggaaaa taatgatgtg gaggtgacag cagaagagct
     1441 tatagcgctg agtgaagcgg cagagcaggc gatgttcacc aaagggatgg aaatccacgt
     1501 tcgccagcgc accatgaaga aggagctgga aaagctgacc agtgcggatg agatactggc
     1561 atacagagtt ggctgggcac aggcgtaata aaaaaggggg gctgataaac agcccctgac
     1621 caaagaaaag tatcagcgga aacatttaga aggatctaaa taaatgacca tgcttgtata
     1681 cggcgtaaag actgccacgg aaatgttaat tctgtacgtg atgctgatct tgtttccgga
     1741 gcatcctgtt gatgtgaata attttcagat tttcggaagt gagagtttgg atatttccat
     1801 atatgccaca gttgatgtgt tttgtggcgc ctggcagtgt gattaaggga acagggataa
     1861 cgaaaaaagc gggggtatca ccaccccccg gtacaaggaa gtattaacta taatggaaac
     1921 attaatgaag acattcagtg ctatgttata tggcaaataa agactgccac agggaagttt
     1981 gcagaaacag tgatgccgat cgtgtttctg tgctcttctc tgataagagt aatttttcag
     2041 atttccggaa gtccgtattt tgcacgggcc cggttgtatg cttcagactg cggtggatat
     2101 caaggtcacg gagttggtcg gttgcaagga atagattgag aattgagcgt gtttctggca
     2161 catgcctgac tgtaatcatc cctacaaact acattgatgc cagaaacgca ctggtaacag
     2221 ggcatcgtgt atttcagtat gtccagtttt tgttgcggtg attgcataaa aaatccccct
     2281 tgagggttga aatatcaccg gaattttcgg caagtatttt tatcgggttg agtgtatttc
     2341 tggggctctg ctgaggcaat tcactgaatg tggcttttgt gctcgctgga aatgcactca
     2401 tacacttttt aacgcgattt ttttcaggca atagttttta atttgttagc gttctcgctg
     2461 tcaggcacgt tttgagcacg tttccgactc tcagctacag caatccacga ctagggacag
     2521 cacttacgtt ttacttagcg caccgtaaaa ctccgctctt cagggtaagg gcttacatga
     2581 agaaaaaaaa taaacttgtg aatatgaaag taaatactat aaaaagatgg ttcctctttt
     2641 ttctcacgtg ctgaataata taaacaatag tgcatgatac aaaagacac
//
LOCUS       DAZFKF010000260         2688 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFKF010000260 DAZFKF010000000
VERSION     DAZFKF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.262"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1183)
                     /locus_tag="ACHKA1_005398"
     CDS             complement(join(<1..859,859..1183))
                     /locus_tag="ACHKA1_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HFO5682906.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRAKKLDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRS
                     PESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LR"
     gene            complement(1274..2527)
                     /locus_tag="ACHKA1_005399"
                     /pseudo
     CDS             complement(1274..2527)
                     /locus_tag="ACHKA1_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(2540..>2688)
                     /locus_tag="ACHKA1_005400"
     CDS             complement(2540..>2688)
                     /locus_tag="ACHKA1_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5682907.1"
                     /translation="NVKVNNESILLPPFSNNDITLKNNHASEYELTVVDDYGNNIHSK
                     IAAR"
BASE COUNT          667 a          666 c          592 g          763 t
ORIGIN      
        1 tgccgtagat attcccgtgg cgagcgataa cccagtgcac tatgcggatg ccattcgtta
       61 taatgctcga acgcctctgc aaggttcttt gctgccgtta acccgtctgg tttgggcatg
      121 acactgatgt agtcacgctt tatcgttttc acgaagctct ctgctatgcc gttactctcc
      181 ggactccgca ccgccgtgct cttcggttca agccccaaca tccgggcaaa ctgccgtgtt
      241 tcattagccc ggtagcatga accattatcc gtcagccact ctactggaga cgccggaagc
      301 tcgttgccga agcggcgttc caccgctccc agcatgacgt cctgtactgt ttcactgttg
      361 aagccgcccg tagtgaccgc ccagtgcagt gcctcacggt cacagcagtc cagcgcgaac
      421 gtgactcgca gtttttctcc gttatcacag cggaactcga acccgtcaga gcaccatcgc
      481 tgattgcttt ctttcacggc cactctgcct gtatgtgccc gtttcgatgg cggtacagca
      541 ggttttcgct caagcaacag cgcattctgg cgcatgatcc ggtaaacacg tttggcattg
      601 atcgcaggca taccatcaag ttctgcctgt ctgcgaagca gcgcccatac ccgacgataa
      661 ccatacgtgg gcagctctcc gataacatgg tgtatacgga gaagcacatc cgtatcatca
      721 gtgtgacgac tgcggcggcc atccatccag tcatcggttc gtctgagaat gacgtgcaac
      781 tgcgcacgcg acacccggag acaacggctg actaagctta ctccccatcc ccgggcaata
      841 agggcgcgtg cgctatccac ttttttgccc gtccatattc aacggcttct ttgaggagtt
      901 cattttccat cgttttcttg ccgagcaggc gctggagttc tttaatctgc ttcatggcgg
      961 cagcaagttc agaggcagga acaacctgtt ctccggcggc gacagcagta agacttcctt
     1021 cctggtattg cttacgccag agaaataact ggctggctgc tacaccatgt tgccgggcaa
     1081 cgagggagac cgtcatcccc ggttcaaagc tctgctgaac aattgcgatc ttttcctgtg
     1141 tggtacgccg tctgcgtttc tccggtccta agacatcaat catctgctct ccaatgacta
     1201 gtctaaaaac tagtattaag actatcactt aaataagtga tactggttgt ctggagattc
     1261 aggggggcag tctactcgta ccttcgtgtc tggtatcatt atttaactta ctactcgatc
     1321 gggtagcgtc aaaagagatg gcacccagcg gtgtattcca accatttccc agagttatag
     1381 cattatagtt atcggataaa atcgtaccgc cataaagtgt caaaagattg ttgaggccat
     1441 atatataatt tgcctcgaga aagtcctcct gattcttgac accatatatc tgactacgtc
     1501 cggcgataaa atcgaaattt gaaacaccag gttgcagcat attgggcacg gaagagtagg
     1561 gcaccaaaaa ggaacgaaca ctaccatctg cttccttaat gctgacatcg agatcagagc
     1621 cgctgcccga aagttgcaaa tctgcgatag taaatggtcc tgggggaacc tctttttggt
     1681 aaatggtata gccattttgc gaaaccgtta tcaaggcgtt actttgcgca acaccttgta
     1741 ctaaaggtgt aaagctttgc atagaatcag gtagcatttg catatcgcga aataagcgta
     1801 ttccgctaaa tcgcagagag tcgaatatga gtgaggatgt gtaattttct cctatctgaa
     1861 ctgtgcttaa tatctgcgac cagccacgtt ccaggtaaag agtattactt tgccatttgc
     1921 ctttcatatc atcaggtttg ctataactgg catcagagtg taactgccaa ctaaataaat
     1981 ttaatccgct gttaaaccgg ccataactgg ctgtattact gttattattg ctgtcataag
     2041 agcgatactg acttaaatta taggaggtat aaaaagcatc aatgccgcga tcccaacttt
     2101 ccggaggaac gtaacctctt tcaagttcat tgatatacgc ctgtggtact gttaaattta
     2161 gtcggtgttc actgatatcc cactgatatt gtccaccatg aaccgcttta tccagtaaaa
     2221 tacaattatc ttctgtattg aggttaccgg tctttacccc cagtttggtt agtagcacct
     2281 taggcagaca tggtttgact ggtgactctg ggatcgtgat atcttgtttt ccacgccact
     2341 ggttatttac ataaaaatca agttcgtagt ttcctggaag cggttgttta ttatcgaaat
     2401 gatattcgga aactttttct cctttcatgc cgccgatcat aaagtgcgta tcaaaagttt
     2461 cttcagcata acccggaaaa gaatacaaaa aaataatagc agcgacccat cgtgtcgtcc
     2521 ttaacatgac tttttcctgt tatctcgcgg cgattttact atggatgtta ttcccgtaat
     2581 catcaactac cgttagttca tattctgatg cgtggttatt ttttaatgta atgtcgttat
     2641 tcgaaaatgg aggcagtaag atactttcat tattaacttt cacgtttt
//
LOCUS       DAZFKF010000261         2657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFKF010000261 DAZFKF010000000
VERSION     DAZFKF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.263"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            33..710
                     /gene="tnpA"
                     /locus_tag="ACHKA1_005401"
     CDS             33..710
                     /gene="tnpA"
                     /locus_tag="ACHKA1_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO5682908.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            710..1057
                     /gene="tnpB"
                     /locus_tag="ACHKA1_005402"
     CDS             710..1057
                     /gene="tnpB"
                     /locus_tag="ACHKA1_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5682909.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            1077..2648
                     /locus_tag="ACHKA1_005403"
     CDS             1077..2648
                     /locus_tag="ACHKA1_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="HFO5682910.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWIGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLSSQ"
BASE COUNT          639 a          727 c          739 g          552 t
ORIGIN      
        1 tctggtctgt ttcctccggc tctacaaaaa taatgtccat catttttaat ggacactatc
       61 gtatgaaaca ccggacctgg atcactgaag ctttacgtct tcactttgaa gaacatttac
      121 cccgggttgt ggccgggcgt cgcctgggtg taccaaaatc aacagtttgt agtatgttcg
      181 tgcgctttcg gagagctggc ctttcgtggc ctttgcccgc aggcatgtcg gagcaggaac
      241 ttgatgcctg cctttacgga caattttcca cggtaccagt cgtacgtcct gaaagcaccg
      301 ttatatccga agcccccgtg gtaaaaaaac gtccccggcg gcccaacttc ccttatgagt
      361 ttaaaatcgc cttagtggag cagtcactgc agcccggagc ctgtgtggcg cagatcgccc
      421 gggaaaacgg aatcaacgat aacctgctct tcaactggcg ccatcaatac cggaaaggtg
      481 gcctgctgcc ttccggaaaa aatatgccgg cactgcttcc cgtgacgtta acgccggagc
      541 cggataataa aatcccggcc cccgcacagg aaccagagca gataaataca ccgtccgaca
      601 gtctgtgttg tgagctggtt ctgccggccg gaactctcag gcttaaaggt aaactgacgc
      661 cggcgttatt acagacactt atccgcgaaa taaaagggag cagccactga tgatatctct
      721 ccctgcaggt tcgcgtatct ggctggttgc aggtatcacc gatatgcgaa atggctttaa
      781 cggcctggca tcaaaagttc agaacgtcct gaaggatgac ccgttctccg gacacctgtt
      841 catcttccgc ggacgccggg gtgaccagat aaaagtgttg tgggctgaca gtgacggact
      901 gtgcctcttc accaaacgcc tggagcgggg ccgcttcgtc tggccagtca cccgtgacgg
      961 caaggtgcac cttactccgg ctcagttatc catgcttctt gaaggtatca actggaagca
     1021 cccgaaacga acggaacgcg ctggaatccg catataaccc gttgtaaagt gaggatatgg
     1081 acacctcact tgctcatgag aacgcccgcc tgcgggcact gttgcagacg caacaggaca
     1141 ccatccgcca gatggctgaa tacaaccgcc tgctctcaca gcgggtggcg gcttatgctt
     1201 ccgaaatcaa ccggctgaag gcgctggttg cgaaactgca acgtatgcag ttcggtaaaa
     1261 gctcagaaaa acttcgtgca aaaaccgaac ggcagataca ggaagcacag gagcgaatca
     1321 gcgcacttca ggaagaaatg gcggaaacgc tgggtgagca atatgacccg gtactgccat
     1381 ccgccctgcg ccagtcttca gcccgtaaac cgttaccggc ctcacttccc cgtgaaaccc
     1441 gggttatccg gccggaagag gaatgctgtc ctgcctgtgg tggtgaactc agttctctgg
     1501 gatgtgatgt gtcagagcaa ctggagctta tcagcagcgc ctttaaggtt atcgaaacac
     1561 aacgtccgaa acaggcctgt tgccggtgcg accatatcgt gcaggcacca gtaccttcaa
     1621 aacccattgc acgcagttat gccggagcgg ggcttctggc ccatgttgtc accgggaaat
     1681 atgcagacca tctgccgtta taccgccagt cagaaatata ccgtcgtcag ggagtggagc
     1741 tgagccgtgc cacactgggg cgctggatag gtgctgttgc tgaactgctg gagccgctgt
     1801 atgacgtcct gcgccagtat gtgctgatgc ccggtaaagt ccatgctgat gatatccccg
     1861 tcccggtcca ggagccgggc agcggtaaaa cccggacagc ccggctgtgg gtctacgtcc
     1921 gtgatgaccg taacgccggt tcacagatgc ccccggcggt ctggttcgcg tacagtccgg
     1981 accggaaagg tatccatcca caaaatcacc tggccggtta cagcggtgtg cttcaggccg
     2041 atgcttacgg tggttaccgg gcgttatacg aatccggcag aataacggaa gccgcgtgta
     2101 tggctcatgc ccggagaaaa atccacgatg tgcatgcaag agcgcccacc tacatcacca
     2161 cggaagccct gcagcgtatc ggtgaactgt atgccatcga ggcagaggtc cggggctgtt
     2221 cagcagaaca gcgtctggcg gcaagaaaag ccagagccgc gccactgatg cagtcactgt
     2281 atgactggat acagcaacag atgaaaacac tgtcgcgtca ctcagatacg gcaaaagcgt
     2341 tcgcatacct gctgaaacag tgggatgcac tgaacgtgta ctgcagtaat ggctgggtgg
     2401 aaatcgacaa caacatcgca gagaacgcct tacggggagt ggccgtaggc cggaaaaact
     2461 ggatgttcgc gggttccgac agcggtggtg aacatgcggc ggtgttgtac tcgctgatcg
     2521 gcacatgccg tctgaacaat gtggagccag aaaagtggct gcgttacgtc attgaacata
     2581 tccaggactg gccggcaaac cgggtacgcg atctgttgcc ctggaaagtt gatctgagct
     2641 ctcagtaaat atcaata
//
LOCUS       DAZFKF010000262         2629 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFKF010000262 DAZFKF010000000
VERSION     DAZFKF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.264"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            20..1378
                     /gene="dgcT"
                     /locus_tag="ACHKA1_005404"
     CDS             20..1378
                     /gene="dgcT"
                     /locus_tag="ACHKA1_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO5682911.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQMIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVLG
                     GPIVAHILSSHYESYDLHIAELTNENGQVVWKTSYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNVSLLFMSRYSVSIWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKDIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMLMDIDHFKKVNDTWGHPIG
                     DQVIKTVVNIIGKSIRPDDLFARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQGNALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
     gene            1476..>2629
                     /locus_tag="ACHKA1_005405"
     CDS             1476..>2629
                     /locus_tag="ACHKA1_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5682912.1"
                     /translation="MLIIDQSAITRFYWQLYYNFLGCITLMRGETCMVFNKKMFVLII
                     IPGILGVLLSFAMSVFQMNRDTTITAGILLKQLDNVTQIVKHTTKLTSILVMKPCKDI
                     LEQLIANGALTPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKNKESRIVSI
                     SGTSFVPGKTAIVFLMPIGNDMTAFSIVESRYIYDLMDVLDDENDDSFSLRFTEGPAI
                     ISGVNNNDRLYMLKKDFNSAISQASLTVTTPMISLYPYVISNVFYILPLSILLSFILY
                     FLWQRWISRKMSLAEEIKKGMSSGEFSVHYQPVCDTTTKACLGVEALMRWQREDGKNI
                     SPVVFIRAAEEENLIIPLTKHLFELIIQDVQSWKVKKPFHLGINIAAEHLA"
BASE COUNT          762 a          437 c          566 g          864 t
ORIGIN      
        1 tacaggttgg tgtagcttta tggaaaaaga ctatttgaga attagtagta ctgtcttagt
       61 gagcttattg tttgggcttg ctttggtttt ggtgaatagt tggtttaatc agccaggcgt
      121 tgaggaagtt gttccacgat caacctatct gatggtaatg attgctttat tttttatcga
      181 tactgttgca tttattttta tgcagttgta tttcatttat gaccgtaggc aattttcaaa
      241 ctgtatactt agtttggctt ttctgagttg tttgatttac tttgttaaaa ccgtcattat
      301 cattcagcaa atgattgagg ggcgtttgac aagcagtgtt gttcaaaatg acattgcaat
      361 ttattatttg tttcgtcaga tgagtttgtg catattaata tttctggcat tggtgaataa
      421 agttagtgaa aacacaaaac agcgcaattt attttccaaa aaaatgactt tgtgcataag
      481 tttgtttttt gttcttgggg ggccgatagt tgctcacata ctttctagcc attatgagag
      541 ctacgattta cacattgccg agttaaccaa tgaaaatggt caagtggttt ggaaaacttc
      601 atatgttacc ataatgattt tcatgtggtt aaccttgctt tcggttaatc tatactttaa
      661 tggtttacgc tatgatatct ggaatggagt gacggtgatt gctttttgtg ccgtactgta
      721 caatgtctct cttttattta tgagcaggta tagcgtgtcc atttggtata ttagtcgcac
      781 tattgaagta gtcagtaagt taaccgttat ggtaatattt atgtgccata tattcagtgc
      841 gctacgagta acaaaggaca ttgcacatcg cgatccctta accaatatat ttaacagaaa
      901 ttattttttt aatgaactga cggttcaatc agcatcggcc aaaaaaacgc cttattgcgt
      961 catgcttatg gatatcgacc acttcaaaaa agtcaacgac acctgggggc atccgattgg
     1021 cgatcaggtg ataaaaacag tggtgaatat cattgggaaa agcatacggc cagatgacct
     1081 ttttgcgcgc gtcggcggcg aagagtttgg cgtcttgctg acggacatcg atactgaacg
     1141 tgcgaaagct ttagcggaaa ggattcggga aaatgttgag cgtttaactg gtgataatcc
     1201 tgaatacgcc ataccacaaa aagtgacgat tagtattggc gctgttgtga ctcagggaaa
     1261 tgcgttaaac ccgaatgaga tctatcgact ggctgataac gcactatacg aggcgaaaga
     1321 gaccgggcgt aataaggtgg ttgtgaggga tgtggtgaat ttttgtgagt caccataaag
     1381 cggcatttta gcgggtgatt ttggtaagcg ctaaatagct attcgcagac atgcgtaaca
     1441 attttcagaa atatcatagt actatatgat cttaaatgct tatcattgat cagtctgcta
     1501 tcacccgctt ttactggcaa ttgtactata atttcttagg ttgcatcacc ctgatgcgtg
     1561 gggaaacatg tatggtattc aacaaaaaaa tgtttgtact cattataata ccaggcattc
     1621 tgggggtgtt actgtcgttt gctatgtcag tatttcaaat gaatcgagat acgacaatta
     1681 ccgccggtat tctgttaaaa cagcttgata atgttacgca aattgtcaaa catacgacaa
     1741 agcttacttc tattctggtg atgaaaccct gcaaagatat cctggaacaa ttgattgcta
     1801 acggagcatt gaccccctat gttcgaacaa cgggtcttat agaaaataat ttccaaatat
     1861 gttcttcagt tagcggtttc aaaaaaatga atgttaacga tgtttacggt acgagctttc
     1921 acaataaaaa taaagaatca cgcatagtat caattagcgg tacaagtttt gtaccaggta
     1981 aaacagccat agtgttcctt atgccaatag ggaatgatat gacagctttt agtattgtag
     2041 agtcacgata tatctatgat ctgatggatg ttcttgatga tgagaatgat gattcatttt
     2101 ctcttcgctt tactgaaggg cctgcaatta ttagtggtgt aaataataat gacaggttat
     2161 atatgttaaa aaaagatttt aattctgcga tcagtcaggc tagtcttaca gttactacgc
     2221 cgatgatttc attatatccc tatgtcatta gtaatgtatt ttacattctt ccattatcta
     2281 ttcttctttc ttttatcctg tacttcttat ggcagcgctg gatatctcgg aaaatgtccc
     2341 tggcagaaga aattaagaaa ggaatgtcat ccggagaatt ttctgtgcat tatcaaccag
     2401 tatgtgacac gacaaccaaa gcgtgtctgg gggttgaggc gttaatgcgt tggcaacgcg
     2461 aagatggtaa aaatatatct ccggtagttt ttatccgcgc tgcagaagag gaaaatttaa
     2521 ttataccgtt aactaagcat ctctttgaac tgattattca ggatgtacaa agctggaaag
     2581 tgaaaaagcc gtttcacctt gggataaata ttgccgcgga gcatcttgc
//
LOCUS       DAZFKF010000263         2587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFKF010000263 DAZFKF010000000
VERSION     DAZFKF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.265"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(136..354)
                     /gene="ymgF"
                     /locus_tag="ACHKA1_005406"
     CDS             complement(136..354)
                     /gene="ymgF"
                     /locus_tag="ACHKA1_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO5682913.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(486..2009)
                     /locus_tag="ACHKA1_005407"
     CDS             complement(486..2009)
                     /locus_tag="ACHKA1_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5682914.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSTTPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(2341..>2587)
                     /gene="ymgC"
                     /locus_tag="ACHKA1_005408"
     CDS             complement(2341..>2587)
                     /gene="ymgC"
                     /locus_tag="ACHKA1_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO5682915.1"
                     /translation="NNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLKD
                     ETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT          791 a          563 c          537 g          696 t
ORIGIN      
        1 caatgatata taaaaacaac tgtgataaca ccagggttga caactatttg cgcaataatt
       61 cctttccttc aatgatccag gcgttcactg tcagcacccc attcagccgc atactgacag
      121 tgagaaactg aaagattatt gctgatccct gtggctgacg agtctttccc ttaatcgatt
      181 aaccagattg atcgccagcc aaacaagggc attaagcaaa tttatcgtca caagcacttc
      241 tcccagcccg gtactgggtt ttgcaataag gatgaccagg agagtgatca gcataaatgc
      301 aatggaaaag atgatataga gagtgaaata atccagatta ttactattgt tcatgctact
      361 aatggcaatc ctgtcaaaac tttcaaacaa ttatagcaaa caaaataatg aacaatagta
      421 acaagaggca acgagctaat tagctgtcta taattacacc gcactatggc cttactgatc
      481 gattttcact caaccacaac cttcaccttc ggtttagaaa ggataatttt gaccaggtcg
      541 atgtatgtaa caggtttata gaaataataa ccctgctgaa atgtgatatt attttggttc
      601 agatagtcaa gttgttcttt cgtttcgaca ccttcagcga cgatactgat cgaaagttta
      661 cgcgccagat ccaatacaca atccagaatt cgggttgatt ctgggtctgc gttgacgcgg
      721 ccaacgaaac tgtgatcaat tttgatataa tcaatatgca ggtcatgcag ataagaaagc
      781 cctgagtagc cagtaccgaa atcatccagc gcgatgacaa aaccattttc atgcagaatg
      841 ttcaaccgct gaaccagact ttcatcaaca ttcaatggct cacgctcggt gacttccaga
      901 acaaggttta aatcgcggcg ggtaaaactg tcacggaaat ttaaacactc gtcgacaaac
      961 gtcggcgaaa taatatgcga agcgctaaaa ttaattccaa tatgaaaacc ttccggcagt
     1021 ttactcgcga tagcgttcat ctgtctggca acctgattca tcaggctttg tgtaagcgga
     1081 acgattaatc cggatttttc agcaagtgga ataaatgccg cgggtgaaat atatccaccg
     1141 tgaggttgtt tccagcgggc taacacctca actccccgca atgtcccttc ccgaccattt
     1201 accacaggtt ggtaaaaagg gacgatctcc ccattattta ttgcccgtcg caggatctct
     1261 tcaggcgttg tgcttttatt gaaatatcta tcaagcagga aggcagcggc gcaggcgatt
     1321 aacagaataa atattaaaat cccaaagcct ttatcgataa gtctgtagaa gctaaagagt
     1381 ggcggcgggt taaagataat agtgaaatga tattttgtgg agtgaacctt ctcggtatag
     1441 ggataatgtc cattaagcgt catcacatca cccgttggtc caataatgtc atccgccacg
     1501 cgtaatacat accttacccc tttcaagggt acattaagcg ccccgcgaat atgttgatca
     1561 cttatcgtaa ccaaaatgcg cgtatctgca aattggctct gatagagcag gataggtaat
     1621 ctattaacgg tgtcgattgc cggagccaac agtaaattac tgtccgggac aacaggaata
     1681 cgagacagca ggacccgatt cccaggcagg gatgtacacc agactattcc ctgtttgaga
     1741 atgatgattg tgcgcaggtg aggtttcaga gctgcttcag tgccaagctg atattgctcc
     1801 tcaaggtcgc attccttacc ggcaatgtta accgctgttt gagtcgcctg tgacgcttct
     1861 tcaagtatat gattgatatt gttggcggca tatcttgctc ccgccaggta ctctgctttt
     1921 gcggaatacc agagctggat gttcaaaata gcgacaccgg taataaacaa gcatatcgca
     1981 acgaggatgg gtatgagtgt attgcgcatt tgcttgtccc ggccaaaggg tttaatacat
     2041 gaaaatgcca ttcattcgct cgcgctggca tctacagaga gcatggtaga gagcataaga
     2101 aatatccacg ccgaagataa aattttctcc atcttgatct aacccattaa ttattgagca
     2161 taatgtaggc agtataaaat aaattaggcg ggatatcagg cgtcaagaat ggagagcgag
     2221 aactctccat tcttgacgcc tgatattgcg gacatattaa gaaagcataa cgcctgaaat
     2281 gctcactttg catcagcatg gtgatacagc tgatgtttat tctaaaacct tactcaagtt
     2341 ctaagagagc acggattccc tgtcattaat aatgatatcc gttagtgtag tcttatgcat
     2401 ttcaatgtac tgcaatattt gcagatagag atcagaaaga attacactgg tttcatcttt
     2461 taaacgcaag cgtaaagcct gatcaataat atgactatca acatacccat gcgtcatgaa
     2521 cacctccctc tcgagatttt taaacaaagc acattgaaaa ataaatctct ctgggattga
     2581 attattc
//
LOCUS       DAZFKF010000264         2571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFKF010000264 DAZFKF010000000
VERSION     DAZFKF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.266"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            247..684
                     /locus_tag="ACHKA1_005409"
     CDS             247..684
                     /locus_tag="ACHKA1_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repA"
                     /protein_id="HFO5682916.1"
                     /translation="MNTQNLIAEHREKHDRDNLGFTQVYPLGWKRLMALAKGNQGAFG
                     LYTFLAQHIDGACGAVVADQAFLADQFDVTTRTVRNWISYLEEQKAIVKIPVAGRVCA
                     YALNPHEVWKGYDNNKPTAAFLTKTLVSKDGEIKRRIMAMFSN"
     gene            1023..1340
                     /locus_tag="ACHKA1_005410"
     CDS             1023..1340
                     /locus_tag="ACHKA1_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V family bacteriocin"
                     /protein_id="HFO5682917.1"
                     /translation="MREITLNEMNNVSGAGDVNWVDVGKTVATNGAGVVGGAFGAGLC
                     GPVCAGVFAVGSSAAVGALYDAAGNSKPTKQKPEGLPPEAWNYAEGRMCNWSPNNLND
                     VCL"
     gene            1360..1572
                     /locus_tag="ACHKA1_005411"
     CDS             1360..1572
                     /locus_tag="ACHKA1_005411"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682918.1"
                     /translation="MRPLTENELKNVSGALYESVNTNCVLASGAMIVSAFGGPIGLIS
                     TGLAAIGACATSSSSSSASASAGNGS"
BASE COUNT          738 a          515 c          626 g          692 t
ORIGIN      
        1 aaaaaaacca aaaccaaacg atcgtgcatt aggaaacata gtgctcttaa gataacaatt
       61 agcggaacat gtaagttatt gtttttattt atagaaatgt agcctgatag gaaacacatg
      121 tgtttccgca tgataggaaa cacatgtgtt tcctatcact tctgaaagta ggaatgaaat
      181 aagcattgat gtggaaaata aatttcctac tataggaatg ataatttccg gttgatagga
      241 atcgctatga atacccagaa tctaatcgca gaacatcgag aaaaacatga cagagataac
      301 ctcggattca ctcaagtgta tccactagga tggaagcggc tgatggctct tgccaaagga
      361 aatcagggcg catttggctt gtatactttt ttggcacagc atatcgatgg tgcgtgcggt
      421 gctgttgtag cggatcaggc ttttctggct gatcagtttg atgtgaccac tcgaactgta
      481 cgcaattgga tctcgtacct agaagagcag aaagccatag tcaaaattcc cgttgcggga
      541 agagtttgcg cctatgctct taacccacac gaggtgtgga aaggctatga caataacaag
      601 ccaacagctg cattcttaac aaagacctta gtcagcaaag atggggaaat taagcgccgg
      661 attatggcca tgttttcaaa ttgagccagc ggcaggcaga caatcagggc tacgtgttaa
      721 cgttttgacc atgattgtct gcctgcattt atccaatggt aataaaattg ttacccaaat
      781 catattttta tatatgcaac ttttaatatg aaatgaacga tcgtattgtg aacgttacta
      841 taaattaagc ataggaatat aaatgaaaac gtggcaagta ttcttcatta tattacccat
      901 aagcattatt attagtcttg ttgcgaaaca gctaaatgct agcaaccttg ttcagtctat
      961 agttagcggt atcgctattg catttatgat atctattttt gttaatagga gataagtgac
     1021 gtatgagaga aataacgcta aatgaaatga ataatgtgtc tggtgctggt gatgtcaatt
     1081 gggttgatgt cggaaaaact gtagctacaa acggagctgg tgtggtcggc ggtgcgttcg
     1141 gagcggggct atgcggccct gtttgtgccg gtgtgttcgc tgtcggatct tctgctgctg
     1201 ttggtgcgtt gtatgatgca gcaggaaatt ccaaaccaac gaaacaaaaa ccagaaggac
     1261 tgccaccaga agcatggaac tatgctgaag gtagaatgtg taattggagt ccaaataatc
     1321 ttaatgatgt ttgtttataa tatgaaaagg aattatttaa tgagaccatt aacagaaaat
     1381 gaattgaaaa atgtatctgg tgctttgtat gaaagcgtta atactaactg tgttttagct
     1441 tctggagcaa tgatagtatc tgctttcggt ggacctatag gtttaatatc tactggatta
     1501 gcagccatag gtgcttgcgc cacttcatca tcgtcatctt cggctagtgc ttctgcaggg
     1561 aacggatctt aactattagg agtattcatg ttcgacgaat tgattaagtt agaacttaaa
     1621 tctatatttt tctcgaaaga atacgtgaat atcatagtta caactattat ttctgtaatg
     1681 ttgatgttca ttttatatca gctacctaat aaagactggt taatattctt tacaggaata
     1741 ttaattatat gggagagaat tcttcttttg aaaataagac gaattcagtc agctaaataa
     1801 ctttcacggt tccggcagcg ccttgagcgc aaccggaaac cgtctgggga gaaccccaga
     1861 cccccatcgc tgcaacatcg acggtctacg accacctacc agccgagtgg ctgtctgttc
     1921 cgtgcaaatg ggctttgcag ggggctggcg cggttcctgg cgacctgaaa gcccctggtt
     1981 aggggtatcg gcgcgcctga cctcctgaaa cgctctgtaa gcgtttttcg cgccttccat
     2041 gccctggcgg gcatttaaac cccgtttcgt gcgtctggga gcttctgagc gcgttttaag
     2101 ggccaatatg tacccaacca ttacagacac tagaaaatcg cgttctggtg ctctggatgg
     2161 cttacagaac gcattttcga acagaacagc aacagaatcg ctgacaacat gcgagaggtg
     2221 acaccgctcg ccgcagcccc agcgaccgaa cgtagtgagc gagtgggcga ggaagcggaa
     2281 gagaaccgga agccacatag agcacttacg cactgatgcg gggtgtcggg gtgaagccct
     2341 gaccagatgg ggaatgtgtg agtgcgcgtg cgcggtccga cattcccaca tcctgtcccg
     2401 atttctgagc cgttttagcg gtgaacggac ggcaaaaaca tgatggagct gttgaacgtg
     2461 gagagcacga aatcggacaa aaatcacggc tgacgaggct tgcagaggag taagccagta
     2521 tacactccgc tatcgctgcg tgactggttc agggcttcgc cccgaaaccc c
//
LOCUS       DAZFKF010000265         2499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFKF010000265 DAZFKF010000000
VERSION     DAZFKF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.267"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            144..371
                     /gene="traY"
                     /locus_tag="ACHKA1_005412"
     CDS             144..371
                     /gene="traY"
                     /locus_tag="ACHKA1_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFO5682919.1"
                     /translation="MIKGALRAAPSRKVLLTLDEATNKLLLGARERSGRTKTNEALVR
                     LQDHLQRYPDFYNEEIFREVTEERESTFKEL"
     gene            406..759
                     /gene="traA"
                     /locus_tag="ACHKA1_005413"
     CDS             406..759
                     /gene="traA"
                     /locus_tag="ACHKA1_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFO5682920.1"
                     /translation="MNTSVSVPGAFTPVKKKSSFFSKLNLLRLVRAVIPAAVLMMFFP
                     QMALAAGGQDLMQSGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLAGFA
                     IISVFIAVGMTVVGL"
     gene            774..1085
                     /gene="traL"
                     /locus_tag="ACHKA1_005414"
     CDS             774..1085
                     /gene="traL"
                     /locus_tag="ACHKA1_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFO5682921.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSFWLRDLIYWYMPTVLLRGIFHNVPDSCFRQWI
                     K"
     gene            1107..1673
                     /gene="traE"
                     /locus_tag="ACHKA1_005415"
     CDS             1107..1673
                     /gene="traE"
                     /locus_tag="ACHKA1_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO5682922.1"
                     /translation="MEHGARLSTSRVLAIAFIFMSLLIVLSLTVNVIQGVNNYRLQNE
                     QRTAVTPMGFNMPFAVSQNSADASFLQQMALAFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNKMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            1660..2388
                     /gene="traK"
                     /locus_tag="ACHKA1_005416"
     CDS             1660..2388
                     /gene="traK"
                     /locus_tag="ACHKA1_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO5682923.1"
                     /translation="MRKNKTVIISACLLFSGSVLAGNGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGETAGAWETSTPYESLLVSISQAVRAGQLPEGWYQI
                     PVTKETLQAPAGMSAKAEAVWTGNHLKMVRFTVENKTLSALNIRESDFWQPGTRAVMF
                     SQPVSQLLVAARMDVYVIRDGEGN"
     gene            2388..>2499
                     /locus_tag="ACHKA1_005417"
     CDS             2388..>2499
                     /locus_tag="ACHKA1_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HFO5682924.1"
                     /translation="MASINTIVKRKQFLWLGIVIVGSVSAIGGALYLSDMD"
BASE COUNT          619 a          548 c          654 g          678 t
ORIGIN      
        1 caaccttcac cgcaaagaaa tgtaagtatt cccggcacta aaagtgccgg tttttgttaa
       61 cgtaatttac tcttttgttg tttgttttgt gtgcgtctta tgtaaatatt ttgactaaat
      121 ctcttttatg tacgggaggt gtggtgatca aaggtgcttt aagagctgct ccaagtagaa
      181 aggttttact gacccttgat gaggcaacca ataaattatt acttggagcc agagagcgca
      241 gtggaagaac gaaaacaaat gaagcattgg tgaggttgca ggatcatttg caacgttatc
      301 cagattttta taacgaagag atattcaggg aggtgacaga agagagagaa tcaactttta
      361 aagaacttta gttttaatct ttttacatac aatccgaggt aattgatgaa tacttctgtt
      421 agtgttccgg gggcttttac gcctgttaaa aagaaatcat cttttttctc taaactgaac
      481 ctgcttcgcc tggttcgtgc tgtgatcccg gcagctgtgc tgatgatgtt cttccctcag
      541 atggccctgg ctgctggcgg tcaggacctc atgcagtccg ggaatgacac agtcaaagcc
      601 acctttggta aggactccag tattgttaaa tgggtcgttc tggctgaagt gctggtcggg
      661 gcggtcatgt acatgatgac caaaaacgtc aagttcctgg ccggttttgc catcatctct
      721 gtatttattg ctgtaggtat gaccgtcgtt ggcctctgac aggaaataaa acgatgtcgg
      781 gagacgagaa taaacttaag aaatatcgtt tcccggaaac actgaccaac cagagccgct
      841 ggtttggcct gccactggat gaactgatcc ccgcagcgat ctgtattggc tggggtatca
      901 caacatcgaa atatctgttc ggtattggtg cggcggttct ggtttatttc gggataaaaa
      961 agctgaaaaa agggcggggc agcttctggt tacgggatct tatttactgg tatatgccaa
     1021 cagtcctgct gcgcggtatt tttcataatg ttcctgattc gtgtttccgg cagtggatta
     1081 aatagaactg atacctggat tgttatatgg aacacggtgc ccgcttaagt accagtcgtg
     1141 tactggccat tgcttttata tttatgtccc tgctcattgt tctcagcctg actgttaacg
     1201 tgattcaggg ggtgaataat taccgccttc agaatgaaca gcgtactgcc gtgacaccga
     1261 tgggatttaa tatgcccttt gcggtatcac agaacagtgc tgatgcctct tttttgcagc
     1321 agatggcgct ggcgtttatt gccctgcgcc tgaatgtctc accggaaatc gtcgatgctt
     1381 ctcaccaggc acttctgcag tatatccgtc cgggcgcgca gaataagatg aaagtgattc
     1441 tggccgaaga agcaaagctc atcaaaaaag ataatgtgaa ttctgctttt ttccagacca
     1501 gtgttcgtgt ctggccacaa tatggtcgtg tggaaatccg tgggattcgg aaaacatgga
     1561 ttggtaactc cgaacctttc actgatatca aacattacat tcttattctg aaacgggaaa
     1621 acggggtgac ctggctggat aatttcgggg aaacagacga tgagaaaaaa taagacagta
     1681 ataatatccg cctgcctgct tttttccggc agcgtgctgg caggaaacgg cacactggca
     1741 cctacagtgg ttccgatggt caatggcggt caggcaagta ttgccatcag taataccagc
     1801 ccgaatctgt ttaccgtccc cggtgaccgg attatcgccg tgaacagtct ggatggtgcc
     1861 ctgaccaata atgagcagac tgcgtccggt ggtgtggtgg tggccaccgt caacaaaaag
     1921 ccttttacgt tcattctgga aacagaacgt ggtcttaatc tttccattca ggccgttccc
     1981 cgtgaaggcg ccgggcgtac cattcagctg gtcagcgatt tgcgcggaac aggagaaact
     2041 gccggtgcat gggaaacatc cacgccttac gaatccctgc tggtttccat cagtcaggcg
     2101 gttcgtgccg gacaactgcc agaaggctgg tatcagatcc ccgtgacaaa ggaaaccctg
     2161 caggcccctg ccggaatgtc cgcaaaagct gaagccgtat ggacggggaa tcacctgaag
     2221 atggtgcgct ttaccgtgga aaataaaacg ctgtctgcgc tgaatatccg ggaaagtgac
     2281 ttctggcagc cgggaacccg ggcggtgatg ttcagccagc ctgtcagcca gttactggtc
     2341 gctgcccgta tggatgtgta cgtcattcgt gacggggagg gcaactgatg gccagtatca
     2401 acaccattgt gaaacgcaag cagttcctgt ggctggggat tgtgattgtc ggatcggtat
     2461 cagcaattgg tggtgcgctt tatctgtctg atatggata
//
LOCUS       DAZFKF010000266         2480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFKF010000266 DAZFKF010000000
VERSION     DAZFKF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.268"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..215
                     /gene="yhhH"
                     /locus_tag="ACHKA1_005418"
     CDS             <1..215
                     /gene="yhhH"
                     /locus_tag="ACHKA1_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HFO5682925.1"
                     /translation="KAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHS
                     DQIAGGVFIIEIGKNDGRILNFGHGK"
     gene            320..1161
                     /gene="yibA"
                     /locus_tag="ACHKA1_005419"
                     /pseudo
     CDS             320..1161
                     /gene="yibA"
                     /locus_tag="ACHKA1_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
     gene            1189..2110
                     /locus_tag="ACHKA1_005420"
                     /pseudo
     CDS             1189..2110
                     /locus_tag="ACHKA1_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            2110..2436
                     /gene="ybfB"
                     /locus_tag="ACHKA1_005421"
     CDS             2110..2436
                     /gene="ybfB"
                     /locus_tag="ACHKA1_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HFO5682926.1"
                     /translation="MKYIIFSFRAIWLALSLLMLFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVADIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
BASE COUNT          787 a          410 c          590 g          693 t
ORIGIN      
        1 ataaagctat agcattggaa ttagctgaga tatatgttaa gaatcgttat ggacaggatg
       61 ccgcagaaga agaaaaacca tacgaaatca ctgagttaac aacaagttgg gttgttgaag
      121 gtaccattca ctcagaccaa attgctggtg gggtttttat tatagaaata ggcaaaaatg
      181 atgggagaat tctgaatttt ggccacggaa aataagtatg taaggataat caaacatcgt
      241 gtgcgctgat ggcagagtag gtggaggact ccagacagtc aaacgataga aaaagatagc
      301 ctttatggag gttcctgcaa tgtcaaatac ataccagaaa agaaaggcaa gtaaagagta
      361 tggtttatat aataatgtaa gaaactaaat gatgatgaat tatttcgctt acttgatgat
      421 cgcaattcct tgaaaaggat ttcatctgcc agagtattac agttaagagg ggggcaagac
      481 gctgttagat tggcaattga attctgcact gacaaaaatt atatccgtag agatatcgga
      541 gcatttatac tcgggcaaat agaaatttgc aaaaaatgcg aagataatgt ttttaatatt
      601 ttgaacaata tggcattgaa tgataagagc gcttgcgttc gagctacggc aatcgagtca
      661 acggctcagc gatgcaagaa aaacccaatt tattcaccta aaatagtaga acaatctcaa
      721 attactgctt ttgataaatc ggctaatgtc agacgtgcta cggcatttgc tatttctgtt
      781 atcaatgata aagaaacaat tccactattg attaatctgt taaaagatcc aaatggagat
      841 gtcagaaact gggccgcatt tgcaataaat atcaataaat atgataatag tgatattagg
      901 gattattttg tggagatgct tcaggataaa aatgaagaag tccgtattga cgcaataatc
      961 ggactttcct acagaaaaga taaaagggtt ttatctgttt tatgcgatga gttaaaaaaa
     1021 aatactgttt atgatgatat cattgaagct gcgggtgaat taggtgataa aacgctactt
     1081 cctgttttag atactatgtt gtacaagttt gatgacaatg aaattataac ttccgctatt
     1141 gataagctga agcgttcata gcgtgagttg cctatgcaca gtgggggatt cccgccggca
     1201 cggtgcagat gctcgaccgg ctggaaagtg aaatcctggc tgagcgggtg agtgaggaaa
     1261 gccgccgctg gctggcatcg tgcggcctga cggtggagca gatgcaaaac cagatggacc
     1321 cggtgtacac gccggcgcga aaaatccacc tgtaccactg cgaccatcgc ggcctgccgc
     1381 tggcgcttat cagtgcggaa ggggctacag aatggtgcgc agaatacgat gaatggggca
     1441 acctgctgaa tgaagagaac ccgcaccagc tgcagcagct tatccgcctg ccggggcagc
     1501 agtatgatga ggagtccggc ctgtattaca accgccaccg ctattatgac ccgctgcagg
     1561 ggcggtatat cactcaggat ccgattgggc tgaagggggg atggaatttt tatcagtatc
     1621 cgttgaatcc gatctcaaat atagatccat taggattaga aacactaaaa tgcattaaac
     1681 cactgcattc aatgggcgga actggtgaaa gaagcggtcc agatatatgg gggaatccgt
     1741 tctatcatca atatctttgt gtcccagatg gtaaagggga ctatacttgt ggtggccaag
     1801 accaacgggg agaatcaaaa ggagatggtc tatgggggcc aggtaaagca agtaatgata
     1861 caaaagaagc tgctggccgt tgtgacctcg ttgaaaccga taatagttgt gtggagaact
     1921 gtttaaaagg gaagtttaaa gaggtaaggc gcgttattct gtattgcctg atatattcac
     1981 acctataaat ttagggctat ttaaaaactg ccaagactgg tctaatgatt ctttagaaac
     2041 atgtaagatg aagtgctccg gaaataacat tggacgcttt attagatttg tattcaccgg
     2101 agtgatgtaa tgaaatatat tattttttca ttcagagcta tatggctggc gttatcactt
     2161 ttaatgctat tcttttccat gcatagactt tcactattag actccactcg tgatgtgagc
     2221 gagttaatta gtctcatgtc ttatggaatg atggtaatat gttttccaac aggcatagta
     2281 ttctttattg ctctaatatt tatagggact gttgcagaca ttattggcgt aaggattgat
     2341 agtaagtata ttatggcgat aataatatgg ctttattttc tgtcaggagg gtatattcaa
     2401 tggtttgtat taagtaagcg cattataaac aaataaatta agttaattgc tcttattatt
     2461 atatgtaacc tggacattga
//
LOCUS       DAZFKF010000267         2475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFKF010000267 DAZFKF010000000
VERSION     DAZFKF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.269"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(185..808)
                     /locus_tag="ACHKA1_005422"
     CDS             complement(185..808)
                     /locus_tag="ACHKA1_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5682927.1"
                     /translation="MQERIDYHIEKYQFRARNESPRLMRQWADVMRECRESGAGARER
                     LRTALLNVDYVTSFELPFRLLLTRTPQMIDTLRREWLLSQKKALFNGRQRGCVYSLQN
                     DLSGVPDCFSYSLATRIRRMDCYGMTTRCFTDIAGQVKAPTARLKLALESGLLVTALD
                     GLFWLGTQRIAADVQRLRQSGMVIVTREVEVSDSLTGTTRLVSAYTL"
     gene            957..1442
                     /locus_tag="ACHKA1_005423"
     CDS             957..1442
                     /locus_tag="ACHKA1_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO5682928.1"
                     /translation="MIKYIFALTLLLSGSVCADQIQGLVIRVLDGDTLEILQARHPVR
                     IRLVNIDAPEKKQPFGRWSMNQLKSLVVNTPVTVTYTQTDRYGRILGRVYTEDGMEVN
                     RWLVQHGAAWVYPDFNTDYTLPAYQREARTMKRGLWADKKPVPPWLWRKENKNSHNNY
                     K"
     gene            2086..2450
                     /locus_tag="ACHKA1_005424"
                     /pseudo
     CDS             2086..2450
                     /locus_tag="ACHKA1_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          711 a          643 c          537 g          584 t
ORIGIN      
        1 ttatcaatat ataaataact gataatagta taatataaag aatcagacca aatctgtttt
       61 tctgcgctcc ggttaatact tctgatttat ctcgcataaa atatcctgtc tgtaatctgt
      121 cccggtaaac aatggtaaat ttcagaatca gtgcgaatac ggtaacaaag attcctcctg
      181 tgctttaaag tgtgtaggca gaaaccagtc tggtggttcc ggtcagggag tctgatacct
      241 caacttcccg ggtgactata accatacctg actgacgcag tctctgcaca tcagctgcaa
      301 ttcgctgagt tcccagccag aacaggccat ccagtgccgt taccagcaac ccgctttcca
      361 gcgccagttt cagccgtgcc gtcggggctt taacctgccc ggcgatatca gtaaaacaac
      421 gcgtcgtcat gccatagcag tccatccgcc gtattcgcgt cgcaagactg tagctgaaac
      481 aatccggcac gccggataag tcattctgca gactgtatac acatccccgc tgccgcccgt
      541 tgaacagggc ttttttctga ctgagcagcc attcccgacg cagagtgtca atcatctgcg
      601 gtgtccgggt taacagcaga cgaaacggca gctcaaaact ggtcacataa tcaacattca
      661 gtaatgctgt ccgcagtcgc tccctggcac ctgccccgct ctcccggcat tcacgcatga
      721 catcagccca ctgacgcatc agtcgtggag actcattcct ggccctgaac tgatatttct
      781 caatatgata atcaatacgt tcctgcatat cgccaccgga tatctgttgt ttacccagac
      841 acagtgatta gcctacagga ataaatatcc tccgtgaagc caggtgcctc agaagataaa
      901 aacatgtact tttttcaacg tgtaatatta acaccacctc cacaggaaaa tataaaatga
      961 taaaatatat attcgcactc acacttctgc tttccgggtc tgtctgtgcc gatcaaattc
     1021 agggactcgt tattcgtgtg ctggacggag atactctgga gatattacag gcccggcatc
     1081 ctgtgaggat tcgtcttgtt aatattgatg caccggaaaa gaaacagcct tttgggcgct
     1141 ggtcaatgaa tcaacttaaa agccttgtgg taaatacgcc cgtaaccgtg acatacacac
     1201 agacagaccg ctacgggcgt attctgggac gggtatatac cgaagacggc atggaagtta
     1261 accgctggct ggtacagcat ggtgcagcct gggtatatcc tgatttcaac acagattaca
     1321 ccctgccggc ataccagaga gaagccagaa caatgaaacg gggtctgtgg gcagacaaaa
     1381 aacctgttcc accatggctg tggcgaaaag aaaataaaaa tagccacaat aattataaat
     1441 aagacatctc acagaaatat atttcctgcc gttttatttc ccctgccagt ccccaacggg
     1501 aaataaccca cagaaattac acagatgtga ataataaacc tgttcgatgt atccgtcaca
     1561 aaagagttga tgtttgactc agaagaaaat acgcctgaag ttaaggaagg cttcagcttc
     1621 atgattgttc tgacatgaac atatatccct ctgtagcgcc ctgcctttca taacatttct
     1681 attacagcaa caacgaaaga caacagcaat gtcggccttt tacgtccaca tgaatattcc
     1741 acttatccgg aattgtgaca cagcccctga atacaggatt gattgccagt gcccacaaat
     1801 ggcacatttt cagtaagatt ctgaatgtta cattcccccg tcatgtgtgg cacaactgtc
     1861 accgggaaag aaacggaaag cacatgaaaa acatcaaaac tcacaccgga ctccttatcc
     1921 acaaagaaca aacgcggcgc gttcgtcttc atgaaactcc ggccgcctgg tgccacacgc
     1981 accgggaatg ctacagcaaa acgaccggtc gtcgctgcgg cagccctgag agtctcagca
     2041 ggctgattct gagcagcatt cagcccatag aatgagatga cccgtatgaa tgatggcaga
     2101 cagacgcctc cgtcccctga ggcatggaaa atccggtttc tggcgaacgg tacacccggc
     2161 acatggtttt acacggatga gcagccgtca caaaagagtg gctgcgggca cattgtgacg
     2221 atgtggaagt gatccccctg tgccgtatgc atgacggtcc cgttttccgg gaacaccata
     2281 ttaatgagct cagcgccgca ctggccagaa tgctccatat gcaccacata ctgctggaac
     2341 gaacccggta cagagaaaat atccatgatg cagaatgtat tcgtgaaatt cacgaagctc
     2401 ccctgcaggc tatcggcatc ctggaaaaat acaaacgcac aaaatcataa ggcagttcta
     2461 tgtggatact gattt
//
LOCUS       DAZFKF010000268         2464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFKF010000268 DAZFKF010000000
VERSION     DAZFKF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.270"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..213
                     /locus_tag="ACHKA1_005425"
     CDS             <1..213
                     /locus_tag="ACHKA1_005425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682929.1"
                     /translation="KKMMLHSILFTQILKIIVCYLINNAELIDVFVVCRLWIISGIKI
                     RSSWLYNHLPVSDTECVQVTGSGRAR"
     gene            1242..1787
                     /locus_tag="ACHKA1_005426"
     CDS             1242..1787
                     /locus_tag="ACHKA1_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HFO5682930.1"
                     /translation="MSKSKALNINKRFSENPFIKDLIIPVKNKQVRISRLGEDNNIMI
                     NQSTGEVLGGTSVVTYKKVDSDNFIKLFTQNIKLAFELTQAGQKALFVLFWAVQNKTN
                     NDLVLLDQYTLNDFINENKGLSMSLPTFKRGLGELVKSQILAMNKRKGFYYINPNFVF
                     NGDRVAFTTVLEMNKSSHKDK"
     gene            complement(1867..2292)
                     /gene="mobA"
                     /locus_tag="ACHKA1_005427"
     CDS             complement(1867..2292)
                     /gene="mobA"
                     /locus_tag="ACHKA1_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO5682931.1"
                     /translation="MAKNKRSGSEVRQRTKVITLRVNNRVASEIRRRAKNEGLTISEH
                     IRTASLNNEIKQRVPSRYLDELIRLGRMQKKLFDKGKRPKDKEYLEVMHKIILLCDEM
                     KIVTKRISDVYNEMDLLKQEIKIIKRHHKNKYPGSNIFS"
BASE COUNT          727 a          491 c          501 g          745 t
ORIGIN      
        1 aaaaaaatga tgcttcattc aatattattt acacagattt taaaaattat tgtttgttat
       61 ttaataaata acgccgaatt aattgatgtc tttgttgtct gtcgtttgtg gattattagt
      121 ggaataaaga ttagatcttc ctggttatat aatcatttgc ctgttagtga tacggagtgt
      181 gtccaagtaa caggaagcgg aagagcaaga tagtagcatg tacataaaat ataaatacgt
      241 gagttattga tatcgcgttt atatattaaa aatataactt aacattgctc ttaatattta
      301 ttttgtcctg acaatgggag cattaatccc cgtgcagtgc tccgccagct gctgacaacc
      361 tcgacatata accatcgata cgatagaaca tcagggcgcg tttccggcaa gaactaacca
      421 tggggatggt ttgcgtgcca acgaacgccg gaaacgcgct tcaaacctac cgagtcgcca
      481 cggttccggc agcgccttga gcgcaaccgg aaaccgtctg gggagagccc cagaccccca
      541 ccgctgcaac accgacggtc tatgaccacc caccaaccga gtagctgcct gtaccgtgca
      601 aatgggcttt gcagggtgct ggcgcggttc ctggcgacct gaaagcccct ggttaggggt
      661 atcggcgcgc ctggcctcct gaaaccctct gtaagcgttt ttcgcgcctt ccatgccctg
      721 gcgggcattt aaaacccgtt tcgtgcgtct gagagcttct gagagcgttt taggggctat
      781 ctggcaactc atccccgaaa gagcaaccgc ggaatcaccg acaacatgcg agaggtaatt
      841 ccgctcgccg caagtcgatg accgagcgta gcgagcgaat cgacgaggaa gcggaagaga
      901 accggaagct acattgagca cttacgcaca gaatttggcc tacgcccccg cgtccgtgca
      961 cgcgtttaat aagaattaag aagggaaatc gcgataaacc gcgccaatcc tagaatctaa
     1021 tgatcaggat attgatctaa cgtatcagaa tagagcatct aacatatcaa ttagttgata
     1081 tttaaagtat tgattgtttg ttttttgctc atcgcgagtg tgatttattt gatctttaag
     1141 gtatcaaaac tatgatacta gtcggatcgg tttattgaca tttgtagatt ttttcttgat
     1201 agtcgaggta tcagtttatt gatacctgag agtgtaaaat aatgagcaag agcaaggctt
     1261 taaacataaa caaacgattt agtgaaaatc ctttcatcaa agatttgata attcctgtaa
     1321 aaaacaaaca agtaagaata tcgcgattag gagaagataa taacataatg attaaccaat
     1381 caactggaga ggttttaggg ggaacctcgg tagtaactta taaaaaagtt gatagtgata
     1441 attttattaa attgtttacc caaaatataa aattggcttt cgagttaaca caagctggtc
     1501 aaaaagcact atttgtttta ttttgggcag tacaaaataa aacaaataat gatctagtct
     1561 tattagatca atacacgcta aatgatttta taaatgaaaa caaagggtta agcatgtccc
     1621 ttccaacatt caaacgtggg cttggggagt tggtaaaatc acaaatatta gcaatgaata
     1681 agcgaaaggg attttattat ataaatccta attttgtttt taatggcgat agagttgcat
     1741 tcacaacagt tcttgaaatg aataaatcga gccataaaga caaatgacaa tgtattaagc
     1801 ccgtcaaagg aaaatttcaa atctattggc gggttttaat attcgtaacg gctagcgccg
     1861 aagattttag ctaaatatat ttgagccagg gtatttattt ttatgatgcc gttttattat
     1921 ttttatttcc tgttttaata gatccatttc attatataca tcagaaattc gcttcgttac
     1981 tattttcatt tcatcgcaaa gtaaaattat tttatgcatc acctcgaggt actccttatc
     2041 cttcggccgc tttcccttat caaataattt cttctgcatc cgaccaagtc ggattaattc
     2101 atctaaatac ctggaaggta cgcgctgttt tatttcatta tttaaacttg ccgtcctgat
     2161 atgttctgaa attgtcagtc cttcgttctt cgctcgccgt ctgatttcgc ttgcgactct
     2221 gttatttact ctaagggtga taaccttagt tctctgccgt acttctgaac cactacgttt
     2281 atttttagcc atcgcaatga ctctccattt tgcacgtttg gggtgtcggg gcttgccctg
     2341 ataggaggta ttctcatgca agcgaatgta ttcgcggtat gacgaataca catcctgtcc
     2401 cgttttccgc tataactttt cgacgaaaat aaaataccgc gtcgtaacct ggtatttctg
     2461 atac
//
LOCUS       DAZFKF010000269         2458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFKF010000269 DAZFKF010000000
VERSION     DAZFKF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.271"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            19..342
                     /gene="yiaW"
                     /locus_tag="ACHKA1_005428"
     CDS             19..342
                     /gene="yiaW"
                     /locus_tag="ACHKA1_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO5682932.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            348..1484
                     /gene="yiaV"
                     /locus_tag="ACHKA1_005429"
     CDS             348..1484
                     /gene="yiaV"
                     /locus_tag="ACHKA1_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5682933.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELNEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1481..2455)
                     /gene="yiaU"
                     /locus_tag="ACHKA1_005430"
     CDS             complement(1481..2455)
                     /gene="yiaU"
                     /locus_tag="ACHKA1_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5682934.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDISDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT          600 a          575 c          629 g          654 t
ORIGIN      
        1 atattctctg agggaaatat gttcctggac tattttgcac tgggagtgct tatttttgta
       61 tttctggtga tattctatgg gattattatt ttacatgata ttccctacct gattgctaaa
      121 aaacgtaatc atcctcatgc cgacgccatt catgttgctg gttgggtgag tctttttacg
      181 ctgcatgtta tctggccatt tttgtggatt tgggccacgc tttaccgccc ggagcgggga
      241 tggggaatgc aaaaccatga ctcctccgtt gtgcaactgc aacagcgcat tgccgggctg
      301 gagaaacagc tcgccgacat caaatcctct tctgccgagt aatacttatg gatttactga
      361 ttattttgac ctatgtggct ttcgcatggg caatgtttaa gatcttcaaa attcctgtaa
      421 ataaatggac cattcccaca gcggccctgg ggggcatatt tattgtcagc ggtttaattc
      481 tgttaatgaa ctataaccat ccgtatacct ttaaagcgca aaaagcggtt atttctattc
      541 ctgttgtccc gcaggtgaca ggcgtggtga tcgaagtgac ggacaagaaa aatacgctaa
      601 ttaaaaaagg tgaggtgcta tttcgactgg acccgacgcg ttatcaggcg cgggtggatc
      661 ggctgatggc ggatatcgtt accgcagaac ataaacagcg ggcgttgggc gcagagttag
      721 atgagatggc ggcgaatact cagcaggcaa aggccacgcg ggataaattc gctaaagagt
      781 accagcgtta cgcacgcggc agccaggcga aagtaaaccc gttttcagaa cgcgatattg
      841 atgtggcacg gcaaaattat ctggcgcagg aagcctccgt aaagtcatcg gcggcggaac
      901 aaaaacagat ccagagccag ctggatagcc tggtgttggg tgaacattct caaatcgcca
      961 gcctgaaagc gcagctcgcg gaagcaaaat ataaccttga gcagacgata gtgcgtgcgc
     1021 cgagcgatgg ttatgtgacc caagtgctga ttcgtccggg tacctatgcc gcgtcgctgc
     1081 cgctacgtcc ggtgatggtg tttatacccg atcagaaacg acaaatcgtg gcgcagttcc
     1141 gtcagaactc cttgctgcgc ctggctcccg gcgatgatgc ggaagtggta tttaacgctc
     1201 tgccaggcaa agtgttcagc ggtaagctgg cagccattag tccagctgtt cccggcggag
     1261 cttatcagtc gaccggcacc ttacagacgt taaacacagc gccgggttca gatggcgtta
     1321 tcgcgaccat tgaactgaat gagcacactg atttgagcgc tttaccagac ggtatttacg
     1381 cccaggtggc ggtctactct gatcatttca gccatgtctc ggtgatgcgc aaagtgttat
     1441 tacgcatgac cagctgggtg cattaccttt atctcgatca ttaatcttcg ctttccgccg
     1501 cttgttcagg ccgcagttgc tgcgactgag caagctggtg gcgaatttca cgcagcattt
     1561 catccagcac atactggacc cgccagggct ggtattcccg tttacggaag atagccacca
     1621 gatccagcca ccattcatac gggtgctgaa agcagggcac cagcgtgccg tcttgcaggt
     1681 cgctctgcac acttttgtct ggcgcaaaca caatccccag gtgatttcgt gccagctcca
     1741 gcgcagattg cgtgttgtcg caaacgtaat ttcccttcac gcgataatcc cgcacctctt
     1801 tactcccggc gacgttgaag cgccagatat tcgcgtcgtc gatcatcatc gagtcgatca
     1861 aaatacagga gtgatgttcg agttcgtcag gacggctaat gggatgtttc tcaagatagc
     1921 gctggctggc ataagcggtt acggcatatt gggtaataaa actggcaacc agcgattcat
     1981 cttttggtgg ggcatagctg attaaaacat cacaatcatc aggaaattcg acgccttcat
     2041 aaaaggcatt acgctcaaga ttgcaggttt ttagcgataa ggtgatatca ctgatatctt
     2101 taattttatc gattacatgt ttggataaat aggtaattat gccggttggg gcgtagatgg
     2161 tgacccgacc acgtttctca tgcttataat ctgcaataaa attattaagc tgctcgtttc
     2221 tgtccagcat gtcgttgatg tacggtaata gcgcggtgcc aaaaggtgtg agcatcagtt
     2281 gtcgggtggt tcggtcaaaa acttttaaac ccacttttga ttcaaaatca gcaaggtatt
     2341 tgctgacgtt ggcctgtgcg atgccaagta cagtcgcggc atggctgata ttttcactgg
     2401 cagcgattac cgagataatt tttaactccc ggtacttaag ttgtaatttt gtcatcgc
//
LOCUS       DAZFKF010000270         2401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFKF010000270 DAZFKF010000000
VERSION     DAZFKF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.273"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(23..1231)
                     /locus_tag="ACHKA1_005431"
     CDS             complement(23..1231)
                     /locus_tag="ACHKA1_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO5682935.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQACGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            1239..1666
                     /gene="tnpA"
                     /locus_tag="ACHKA1_005432"
                     /pseudo
     CDS             1239..1666
                     /gene="tnpA"
                     /locus_tag="ACHKA1_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
     gene            complement(1759..>2401)
                     /locus_tag="ACHKA1_005433"
     CDS             complement(1759..>2401)
                     /locus_tag="ACHKA1_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO5682936.1"
                     /translation="AALCGLLALSGCASKITQPDKYSGFLNNYSDLKETTSATSKPVL
                     RWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIAQRKPV
                     VTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRLYFEGE
                     LIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK"
BASE COUNT          568 a          603 c          570 g          660 t
ORIGIN      
        1 aggacgtgga tccctgctac gttcaggctg tcgcctgaat catttcggtg ggttcctgct
       61 tcaacgggcg gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta
      121 aaatgttacg agcgccattt acatcagcgt tcgctgtata tccacatgcc tggcatctga
      181 atttactttg tgacaggcga ttttctttcg ctgtatgacc acagcacgcg caacgctggc
      241 ttgtgtacgc tggcggaaca gcaagcacct gaccgccacg ccagagctgc ttgtactcaa
      301 gctggcggcg catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg
      361 cccggacatt gcgccccggc tggctgaccg tacccgctgc tgactttgac atgttgctga
      421 ccttcaaatc ctcaatgact atcattgcgt ggtttttgct gacggtcgtt gtgactttgt
      481 gaaggtagtc cctgcggata tttgcgatac aggaatgcag tcgctgtatt ttgcgtttct
      541 gcttctgcca gttgttgctg aatttgacct tgcggcttaa ctgtcgctga agtctcgcca
      601 gcgtcttctg gtttttctga aaactgttta caggcccaaa gactgtgcca tctgacagcg
      661 tggcgagttt agccacgcca gcatccagtc cgaccattaa tgctgaaggg tgaaccggag
      721 ttgatacttc attttcagtc tgaatactga tgtaccactt accgcaggac tggctgacag
      781 tgacattttt cacaacaccc gtgacctgac ggctattccg gtagcgcatc cagccaagtt
      841 tcggcagaaa aatacgactg ttttcctgat cgagcttaac gccctgcggg tagcggaatg
      901 catcattctg tccccgcttt ttgaatcggg gaaaagcagc ccgcttccgg aagaagtttt
      961 tgtaagcccg ctcaaggtct ttcagtgact gttgcaatgg ctgtgacggg gcatctttaa
     1021 gccattgcgt ttcagtggca tttttccact caaccagcca ggaagccatt ttcccgtaag
     1081 ggatgtattt attaccggcc tcatgattct cattctgaag cgccagagca cgattgaaaa
     1141 cgaaacgaca tgcgcctgcg aagcgcctca tttcacgctc ctgttgacca ccgggtctta
     1201 actggaattt aaatgcctgt agtcgtttca caggccaaat gatactttgg tctgtgaaaa
     1261 aagaaaccga tatccggcgt ggaaggcatt gtgttttcct gatgcatgtt cacctggtct
     1321 ttgtcaccag ataccgacgc cagatttttg accatgacgc gacagaaaaa ctacgcactt
     1381 acttttcaaa ggtatgtgct gattttgaag ctgaactggt tgaaatggat ggcgaaccag
     1441 atcacgtttg ttaattaact atcctcccaa actggcgata tccagtctgg taaacagcct
     1501 caaaggcgta tcgggtaggt tactgcgacg agatcgacca gatattgcag tcaggtatta
     1561 ctacaaaggc gttttgtgga gtcctggcta ttttgccagt agctgcggag gtgcgccaat
     1621 atccgccatc cgtcaataca ttgaacaaca gcaaacacct ggttaggtgg aaaaccgcgc
     1681 cgtatatccc cgccctgaag gacggggttt tacggcgcac cggataaaaa gcgccatcta
     1741 acgatggcgc gtctggaact actttttgtt aacgtcaaac atggtggcat cggtcgccat
     1801 gtcatcaata acttgtttaa tattttcgaa agccattggc gtactttcgt tattcaggtc
     1861 tttaccttcg ccctgacgaa cgactttgat aaccggttta ttagtcgctg catcaatcag
     1921 ctcaccttca aaatagaggc gagtatccat ggtacggtgg cctgtagcca tttgcgtccc
     1981 cgcaaccact aatgcaacag gaaccacttc atagaattgc agcccttctt tgctggtatc
     2041 tacaccggta atggccccac ggaaaatcag actacgcggc ccagcggtgg taaccactgg
     2101 tttacgctgc gctatcgctt ctttcatttc ggtgttggta tagttcaaaa ttttatccag
     2161 aactttctgc cctacctggg tagaaggttt tggtaccgga taataagtga ttgggttcca
     2221 gacgatgctg tcatatttgc tttgatcaaa actcgggtct acccaacgta aaacaggttt
     2281 acttgttgct gaggttgttt cttttaaatc agagtaattg tttaaaaaac cagaatattt
     2341 atctggctgg gtgattttag aggcacaacc agacagagcc aacaagccgc ataaagctgc
     2401 a
//
LOCUS       DAZFKF010000271         2397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFKF010000271 DAZFKF010000000
VERSION     DAZFKF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.274"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..221)
                     /locus_tag="ACHKA1_005434"
     CDS             complement(<1..221)
                     /locus_tag="ACHKA1_005434"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682937.1"
                     /translation="MTLPPFRDFLCSVYYMQLVDESFKTNAMQDRQSTRNCMQCSVDC
                     PPAAGSMVIFFDIYLAQLQVLEFFSLLFF"
     gene            complement(959..1222)
                     /locus_tag="ACHKA1_005435"
     CDS             complement(959..1222)
                     /locus_tag="ACHKA1_005435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682938.1"
                     /translation="MSDRARALRHSPPGQGFTPTPRISAYVLNVTSGSLPLPRPLAHS
                     RSVAGAAASGTTSRMLSAILLLFCSKMRSVSHPEHQNAIFWCR"
     gene            1631..2170
                     /locus_tag="ACHKA1_005436"
     CDS             1631..2170
                     /locus_tag="ACHKA1_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HFO5682939.1"
                     /translation="MLEQQFEDFEQQKRRDHQVQDQSKNAKQKRDRLNPPDWSQVTKA
                     GWNMLDAISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDL
                     ESIGAIVKIPVGIYYAYALDPYQVWRGYNNTKKYAAFNAKTLTQKDGVIDRKLRLMLS
                     RREEFESSLKYSNHDSEQG"
     gene            2177..>2397
                     /locus_tag="ACHKA1_005437"
     CDS             2177..>2397
                     /locus_tag="ACHKA1_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682940.1"
                     /translation="MRIKYESRWRLEELKLEICSSIDELINKYDIEYATGINLYMNCE
                     DKEFNPVDVINEDGNKIMFVVGNNEKIQKP"
BASE COUNT          747 a          496 c          564 g          590 t
ORIGIN      
        1 aaaaaaaata gcaaagaaaa aaactccagc acctgtaatt gagctagata aatatcgaaa
       61 aaaatgacca ttgagccagc ggcaggtgga caatccacag aacattgcat gcaatttcta
      121 gtggattgtc tatcctgcat cgcattggtt ttgaacgact catcaacgag ctgcatgtag
      181 taaacgctac ataaaaaatc cctgaatgga gggagcgtca taatgagatt cctgtttttc
      241 tttcgatgca gaaggcgcaa aaaaatcccc tgatgtattt caggggacgc aagaggaata
      301 gcgagataat tatcatagga ctaacttcga acatcattga cctgttacgt gctggagaga
      361 acacataaca agcttgaatt tgccggaaaa agtcgcacaa aacaaccctg gatgtttgtg
      421 accacgcgac acaaccagac aaacatcatc cgggcataat ttcatctcga caccgtttct
      481 gcgatgcgat agaacatcaa ggcgcatttc cggcaaccaa ctgaccgtgg gtatggtttg
      541 cgttagcagc aagcgccgga aatgcgcgcg attcgctgag ttttactccg cacattaaaa
      601 aaatccccgt gtcgaacaat ctacaatttc ccctattcct tcacggttcc ggcagcgcct
      661 tgagcgcaac cggaaaccgt ctggggagaa ccccagaccc ccatcgctgt aacaccgacg
      721 gtctacgacc accaaccagc cgaatagttg cctatgccgt gcaaattggg ctttgcaggg
      781 ggctgacgcg gttcctggcg acctgaaagc ccctggttag gggtatcggc gcgcctgacc
      841 tcctgaaacg ctctgtaagc gtttttcgcg ccttccatgc cctgacgggc atttaaaacc
      901 cgtttcgtgc gtctgagagc ttctgagcgc gttttaaggg ccgatacgta cccaatcatt
      961 accgacacca gaaaatcgcg ttctggtgct ctggatggct tacagaacgc attttcgaac
     1021 agaacagcaa cagaatcgcc gacaacatgc gagaggtggt accgctcgcc gcagccccag
     1081 cgaccgaacg cgagtgagcg agtgggcgag gaagcggaag agaaccggaa gttacattga
     1141 gcacgtacgc actgatgcgg ggtgtcgggg tgaagccctg accaggtggg gaatgtctga
     1201 gtgcgcgtgc gcggtccgac attcccacat cctgtcccga tttctgaggc gttttagcgg
     1261 tgaacggacg gcaaaaccat gatggagctg ttgaacgttg agagcgtgaa atctggcaaa
     1321 aatcacagct ggtaaggctt gcagaggagt aagccagtat acactccgct atcgctacgt
     1381 gactgggtca gggcttcgcc ccgacacccc aaaagcagtt aggtagtgtt aaatcatgag
     1441 cagtaaaaaa acgctttgat agatctttag agcaaaaatc gacatttcta taaaaatcat
     1501 tagcttaaga caccatcata cgaaacgtta tttgcatgtg tatacgcaaa catgtttgca
     1561 tgtgttgttt cgtactgtac gatataaatt tcgtaaggta cgaaatatca ttgcaggtaa
     1621 ggatgcaagt atgttggaac agcaatttga agactttgaa cagcaaaaac ggcgagatca
     1681 tcaggtacaa gatcaatcca agaacgctaa gcaaaaacga gatcggttga atcctccaga
     1741 ctggtcgcag gttactaagg ctggttggaa tatgttggac gctatatcag aaaaagttcc
     1801 tagagcattt cggttatatt ctttttttgc ccagaatata gataaatcat gtggggctgt
     1861 tgtatgtgat caaaaattct tagctgatta tttcggtgtt agtttaagaa cgatccaata
     1921 ttggattgga gatttagaaa gtattggcgc aatagttaaa ataccagtag gtatatatta
     1981 tgcctatgca ttagatcctt atcaggtatg gcgtggatat aacaatacta aaaaatatgc
     2041 agcatttaac gcaaaaacat taactcaaaa agatggggtt atagatcgta aattgcggct
     2101 tatgctttct agacgtgaag agtttgaaag ttccttaaag tactctaatc atgactcaga
     2161 gcaggggtga tgcactatga gaattaaata tgaatcacgt tggagattag aagaactaaa
     2221 attagaaata tgcagttcaa tagatgagtt aattaataaa tatgatattg aatatgcaac
     2281 aggaataaat ctttatatga attgcgaaga taaagaattt aatcctgttg atgttatcaa
     2341 tgaagatgga aataaaataa tgtttgtcgt tggcaacaat gaaaaaatac aaaagcc
//
LOCUS       DAZFKF010000272         2392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFKF010000272 DAZFKF010000000
VERSION     DAZFKF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.275"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            64..444
                     /locus_tag="ACHKA1_005438"
     CDS             64..444
                     /locus_tag="ACHKA1_005438"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5682941.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            441..788
                     /gene="tnpB"
                     /locus_tag="ACHKA1_005439"
     CDS             441..788
                     /gene="tnpB"
                     /locus_tag="ACHKA1_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5682942.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            838..2376
                     /locus_tag="ACHKA1_005440"
     CDS             838..2376
                     /locus_tag="ACHKA1_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFO5682943.1"
                     /translation="MNDISSDDILLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGRITEAACWAHA
                     RRKIHDVHVRIPSALTEAALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKN
                     FLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIA
                     LPAE"
BASE COUNT          553 a          665 c          696 g          478 t
ORIGIN      
        1 gaatctgacg aattagacaa agtggtgtcc accaaataag tagtgggaac caaagtgtca
       61 gatatgcaga aaaatgtgac tcccggcagg cgaaaaggct gccctaatta tcctcccgaa
      121 tttaaacagc agctcgttgc tgcctcctgt gaacccggga tatccatctc aaaacttgct
      181 cttgaaaatg gcattaacgc caatctgttg ttcaaatggc gacaacaatg gcgcgaggga
      241 aagctgctat taccttcttc agagagcccc cagctacttc ctgtgactct cgatgcagct
      301 gccgaacagc cagaatcgct cgcagaggac ccggaaaccc tcagtatcag ctgtgaggta
      361 acgttccggc acgggacgct ccgcttcaat ggcaatgtca gcgaaaagct cctgactctg
      421 ctgatacagg aactgaagcg atgatcccgt taccttccgg gaccaaaatt tggctggttg
      481 ccggtatcac cgatatgaga aatggcttca acggcctggc tgcgaaagta cagacggcgc
      541 tgaaagacga tcccatgtcc ggccatgttt tcattttccg gggccgcagc ggcagtcagg
      601 ttaaactgct gtggtccacc ggtgacgggc tgtgcctcct gaccaaacgg ctggagcgtg
      661 ggcgcttcgc ctggccgtca gcccgtgatg gcaaagtgtt ccttacgcag gcgcagctgg
      721 cgatgctgct ggaaggtatc gactggcgac agcctaagcg gctgctgacc tccctgacca
      781 tgctgtaaat ctctttatcc tggttgtcac agaataagcc cggtaaaata cgggcttatg
      841 aacgacatct cttctgacga catcctcctg ctgaaacagc gcctggccga acaggaagcg
      901 ctgatccacg ccctgcagga aaagctgagc aaccgggagc gcgaaataga ccatctgcag
      961 gcgcagctgg ataaactccg ccggatgaac ttcggcagtc gttccgaaaa agtctcccgc
     1021 cgtatcgcac aaatggaagc cgatctgaac cggcttcaga aagagagcga tacgctgact
     1081 ggtagggtgt atgacccggc agtacagcgt ccgttgcgtc agacccgcac ccgtaagccg
     1141 ttccctgaat cactaccccg tgacgaaaag cgactgttgc ctgcagcgcc gtgctgcccg
     1201 aactgcggcg gttcactgag ctatctgggc gaggataccg ccgaacagct ggagttgatg
     1261 cgtagcgcct tccgggttat ccggacggta cgggaaaaac atgcctgtac tcagtgcgat
     1321 gccatcgtgc aggcacctgc accttcgcgg cccatcgagc ggggtatcgc cggaccgggg
     1381 ctgctggccc gcgtgctgac ctcgaagtat gcagagcaca ccccgctgta tcgccagtca
     1441 gaaatatacg gccggcaagg tgtggagctg agccgttcac tgctgtcggg ctgggtggat
     1501 gcatgctgcc ggctgctgtc tccgctggaa gaggcgcttc atggctatgt catgactgac
     1561 ggcaaactcc atgccgatga taccccggtc caggtactgc tgccgggtaa taagaagacg
     1621 aagaccgggc ggttgtgggc gtatgttcgt gatgaccgca atgccgggtc agcgttggca
     1681 cctgcagtgt ggttcgctta cagcccggac agaaaaggca tccatccgca gactcatctt
     1741 gcttgcttca gcggtgtgct gcaagcggat gcgtacgccg ggttcaacga gctgtatcgc
     1801 aatggtcgga taacggaagc tgcctgctgg gctcatgccc gccgaaagat ccacgatgtg
     1861 cacgtccgca tcccgtcagc actgacggaa gcagccctgg agcagatcgg tcagttgtac
     1921 gccatagagg cggatataag gggaatgccg gcagagcagc ggcttgctga acgtcagcga
     1981 aaaacgaaac cgctgttgaa atccctggaa agctggttgc gtgaaaagat gaagaccctg
     2041 tcgcgacact cagagttggc gaaggcgttc gcgtacgcac ttaaccagtg gccggcactg
     2101 acgtactatg cgaacgatgg ctgggtggaa atcgacaaca acatcgctga aaatgccctg
     2161 cgggcggtca gtctgggtcg taaaaacttc ctgttcttcg gctctgacca tggtggtgag
     2221 cggggagcgc tactgtacag cctgatcggg acgtgcaaac tgaatgacgt ggatccagaa
     2281 agctaccttc gccatgtgct tggcgtcata gcagactggc cggtcaaccg ggtcagcgaa
     2341 ctgcttccgt ggcgcatagc actgccagct gaataacaca tccccgtcaa ta
//
LOCUS       DAZFKF010000273         2293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFKF010000273 DAZFKF010000000
VERSION     DAZFKF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.276"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            141..356
                     /gene="essQ"
                     /locus_tag="ACHKA1_005441"
     CDS             141..356
                     /gene="essQ"
                     /locus_tag="ACHKA1_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5682944.1"
                     /translation="MKSMDKLTTGIAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKVARRE"
     gene            361..696
                     /locus_tag="ACHKA1_005442"
                     /pseudo
     CDS             361..696
                     /locus_tag="ACHKA1_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
     gene            complement(860..1120)
                     /locus_tag="ACHKA1_005443"
     CDS             complement(860..1120)
                     /locus_tag="ACHKA1_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682945.1"
                     /translation="MDYTNLPKQTFADLIALRQAVVALINLLPEKEKELVKALLNRTA
                     ADFSSYPLTDDLADLPELIAASAIKLTEEIYPPQKSSQNSCE"
     gene            1234..1767
                     /locus_tag="ACHKA1_005444"
     CDS             1234..1767
                     /locus_tag="ACHKA1_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5682946.1"
                     /translation="MNVKIRYSLSAAVLALIAASAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCAQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEH"
     gene            1764..2261
                     /gene="rzpQ"
                     /locus_tag="ACHKA1_005445"
     CDS             1764..2261
                     /gene="rzpQ"
                     /locus_tag="ACHKA1_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 family protein"
                     /protein_id="HFO5682947.1"
                     /translation="MNQIFTVILLVLVGFVVGNVWSDRGWQKKWAERDAAELSQEVNV
                     QFAARIIEQGRTISRDEAVKDAQQKAAEISARAADLSDSVNQLRAEATKYAIRLDAAQ
                     HTANLAAAVRGKTTKAAEGMLTNMLGDIAAEAQLYAEIADKRYIAGVTCQRIYESLRD
                     KKYQM"
BASE COUNT          641 a          461 c          594 g          597 t
ORIGIN      
        1 tttcttttaa aataattacc cacattttta atgtgtctgt tttttagcgc cgttgagaac
       61 aacgtttgct ataaaaacta acccatagac tccgatcttt tcaaacatat tgcaccatct
      121 gtgtacatcg gggtgaggat atgaaatcaa tggataagtt aacaacaggt atcgcctatg
      181 gcacatcggc tggtaatgct ggtttctggg cattgcagtt actcgataaa gtaactccgt
      241 cacagtgggc tgcaatcggt gtgctgggta gtctggtttt tggcctgctg acgtatctga
      301 caaaccttta tttcaagatt aaagaagaca ggcgtaaggt tgcgagaaga gagtaatcca
      361 atgacacaag actatgaact ggttgtgaaa ggagtccgca attttgagaa taaagtggcg
      421 gtaactgtag ccttacggga caaaaaacgc tttgacggtg aaatttttga cctggacatc
      481 tcgctggacc gtgttgaagg tgccgcgctg gagttttatg aggcagcagc cagaatgaga
      541 atcagacagg tattcctgga tgttgctgcc gggttatgtg taggggatga gcagtcgccg
      601 gaaaagcgcc ccataatttt agaggcgcag aatgtgtgga taacctacaa aggaaagcta
      661 ccgggaagaa ttactggttc tctgaagacg ccaccgacgg cattgcggtc agaaaaagat
      721 gatattgaat cgcccattga aaaactggag ggcagcgtcg ctgatctgaa taaaaaattg
      781 tcggtgctga tcccttctga agatgaaaaa aaacgccgcg atgagcagtt tgcggcgttt
      841 tacgattatt gcattgaagt tactcgcagg aattttgtga agatttttga ggagggtaaa
      901 tctcttcagt aagcttaatg gcggacgctg caattaattc aggaaggtcc gcaaggtcat
      961 ctgtcagtgg atatgatgaa aaatcggcgg cagttctgtt aagaagcgct ttaactaatt
     1021 ccttttcctt ctccggcaac aagttgatta gagctacgac tgcttgcctg agtgcgatta
     1081 aatcagcaaa agtttgtttt ggtagatttg tgtaatccat agtcacctct gtgtttatca
     1141 gattgacatc ctcctcccgc cagtgcccat cactggtgag gtaagattta acatatccgg
     1201 ggatttgaag ccgataaatc ctgataaata tccatgaacg taaaaatcag atacagcctg
     1261 tcggctgctg ttctggcact gattgccgcc agtgctcctg cccctgacat tctcgatcag
     1321 tttctggatg aaaaagaggg gaatcacacc actgcatacc gcgatggctc cggcatatgg
     1381 accatctgtc ggggagcaac gatggtggac ggtaaacctg ttattccggg aatgaaactg
     1441 tcgaaggaaa aatgcgccca ggttaacgcc attgagcggg ataaggcgct ggcatgggtg
     1501 gagcgcaata taaaagttcc actgaccgag ccacagaaag ccggtatcgc gtcattctgt
     1561 ccctataaca ttggccctgg taagtgtttt ccgtcgacgt tttataagcg gcttaatgcc
     1621 ggtgatcgta aaggcgcatg cgaggcgatt cgctggtgga taaaggacgg tggacgtgat
     1681 tgccgcattc gttcaaataa ttgttacggt caggttattc gtcgtgacca ggagagtgca
     1741 ttaacctgct gggggataga acattgaatc agatattcac ggtgattttg ctcgtgttgg
     1801 taggatttgt cgtaggtaat gtctggagcg acagaggatg gcaaaaaaaa tgggcggagc
     1861 gtgatgctgc cgaattatct caagaggtaa atgtccaatt tgctgctcga ataattgaac
     1921 aggggcgaac tatatcccgt gatgaggctg ttaaagatgc acaacagaaa gccgctgaaa
     1981 tttctgccag ggctgctgat ctgtctgata gtgttaacca gctgcgtgcc gaagcaacaa
     2041 aatatgccat acgccttgac gcagcgcagc ataccgcaaa tcttgccgct gccgtcagag
     2101 gcaaaacaac caaagccgcc gaaggaatgc tcaccaacat gctcggagat attgcagcag
     2161 aagctcagct ttatgctgaa attgctgaca aacgctacat cgcaggagtg acttgtcaac
     2221 ggatttatga atctttaaga gataaaaaat atcagatgta gattaatatt aaatcggatt
     2281 atttttagcg ctg
//
LOCUS       DAZFKF010000274         2243 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFKF010000274 DAZFKF010000000
VERSION     DAZFKF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.277"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            230..>2243
                     /gene="fadH"
                     /locus_tag="ACHKA1_005446"
     CDS             230..>2243
                     /gene="fadH"
                     /locus_tag="ACHKA1_005446"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO5682948.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAMQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ANQLQEFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALE"
BASE COUNT          516 a          623 c          649 g          455 t
ORIGIN      
        1 tctgcaccgc gccactggcg gatgcggcgt gaacgcctta tccgccctac atgtgcaatc
       61 ccccaggtcg gataggaccc ggcaagcgtc gcatccggcg ttaccaccgg gcgtattctt
      121 tttgaatccc atcacaaacc ccgcactccc cttttccctt ttctccggcg acggctaaat
      181 tagaactcat ccgaccacat aacaattatt ttacatactg gacaatttta tgagctaccc
      241 gtcgctgttc gccccgctgg atttaggttt taccacgtta aaaaaccgcg tgttgatggg
      301 ttcaatgcac accgggctgg aggaataccc ggacggcgct gaacggctgg ctgcgtttta
      361 tgccgaacgc gcccgtcacg gcgtggcgct gattgttagc ggcggcattg caccagattt
      421 aacaggcgtt ggcatggaag gcggcgcaat gctcaatgac gccagccaga tcccacacca
      481 tcgcaccatt accgaagccg tacatcagga aggcggcaaa atagccctgc aaattttgca
      541 taccgggcgc tacagctacc aaccgcatct ggtcgcaccg tccgcattgc aggcccccat
      601 caaccgtttc gttcctcatg aactcaccca tgaagagatc ctgcaactga tcgacgattt
      661 cgcccgctgc gcgcaactgg cgcgggaggc aggatacgac ggtgtagagg tgatgggttc
      721 cgaagggtat ttgatcaacg aatttctgac gctgcgcacc aatcagcgta gtgaccagtg
      781 gggcggcgat taccacaacc ggatgcggtt tgccgttgaa gtggtgagtg cggtacgcga
      841 acgcgttggc aacgacttca ttattatcta ccgactgtcg atgctcgacc tggtcgaaga
      901 cggcgggact tttgccgaaa cggtagagct ggcgcaggcc attgaagcgg cgggcgcaac
      961 cattatcaac accggcattg gctggcatga agcacgtatt ccgaccattg ccacgcccgt
     1021 gccgcgcggc gcatttagct gggtcacgcg caaactgaaa ggccacgttt cgctgccgct
     1081 ggtgaccacc aaccgaatta acgatccgca ggttgccgac gacattctct cgcgcggcga
     1141 tgccgatatg gtgtcgatgg cacgaccgtt tcttgctgat gcggagctgc tgtcaaaagc
     1201 gcaatcaggg cgagccgatg agatcaacac ctgcattggc tgcaatcagg cctgcctcga
     1261 tcaaatcttc gttggcaaag tcacctcgtg cctggtgaat cctcgtgcct gccatgaaac
     1321 caaaatgccg atccttcccg ccatgcagaa aaaaaatctg gcggtggtcg gtgcgggacc
     1381 agctgggctg gcgtttgcca ttaacgcggc ggcgcgtggg catcaggtaa cattgtttga
     1441 tgcccacagc gagatcggcg ggcagtttaa tatcgccaaa cagatccccg gcaaagagga
     1501 gttttacgaa acgctgcgct actaccggcg gatgatcgaa gtaacgggcg tgacgctgaa
     1561 actcaatcac accgtaacag cgaatcagtt acaggaattc gatgaaacga tcctcgccag
     1621 tgggatcgtg ccgcgcactc cgcccatcga cgggatcgat catccgaagg tattgagtta
     1681 tctcgatgta ctgcgcgaca aagcgccggt gggcaacaaa gttgccatca tcggttgtgg
     1741 cgggattggt tttgatacgg cgatgtattt aagtcagccg ggcgaatcca ccagccagaa
     1801 tatcgctgag ttttgtaatg agtgggggat cgacagtagc ctacaacagg ctggtggctt
     1861 aagcccgcag ggaatgcaga tcccgcgtag cccacggcag attgtgatgc tccagcgcaa
     1921 agccagcaaa ccgggacagg ggttaggcaa aaccaccggc tggatccatc gcaccaccct
     1981 gctctcgcgg ggtgtgaaaa tgatcccagg cgtaagttat cagaagattg acgatgacgg
     2041 gctgcatgtg gtgatcaacg gcgaaacgca ggtattagca gtggacaatg tggtgatctg
     2101 cgcagggcaa gagccaaacc gcgcgctggc gcagccgctg attgatagcg ggaaaacggt
     2161 gcatttaatt ggcggctgcg atgtggctat ggagctggac gcacgacggg caattgccca
     2221 gggaacacgg ctggcgctgg aga
//
LOCUS       DAZFKF010000275         2173 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFKF010000275 DAZFKF010000000
VERSION     DAZFKF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.278"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(115..>2173)
                     /locus_tag="ACHKA1_005447"
     CDS             complement(115..>2173)
                     /locus_tag="ACHKA1_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5682949.1"
                     /translation="AEQWQYDGHGWLTDISHLSEGHQVAVHYGYDDKGRLAGERQTVH
                     NPETGELLWQHETEHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGGTPLLEFT
                     RDRLHRETVRSFGNNAYELTSTYTPTGQLQSQHLNSLVYDRDYGWNDNGDLVRISGPR
                     QTREYGYSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPELHPDSTLTVWPDNRIT
                     EDAHYLYRYDEYGRLTEKTDRIRAGVIRTDDERTHHYHYDSQHRLVFYTRIQHGEPLV
                     ESRYLCDPLGRRMAKRVWRRERDLTGWMSLSRKPEETWYGWDGDRLTTVQTDTTRIQT
                     VYEPGSFTPLIRVETENGERKKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLDRLEE
                     EIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHLYHCDHRGLPLALISEDG
                     NTAWRGEYDEWGNQLNEENPHHVYQPYRLPGQQYDEESGLYYNRNRYYDPLQGRYITQ
                     DPIGPVGGWNPYSYVLNNPAQFIDPTGLTQEDNWTYGEVGNSCVTSQRDFTPPPGLAD
                     MKASIGAACSRNNASRNCHPVDGSNASAPSDKAAWENIQNATNGKDLSGGGNMFCVGN
                     EDCAVVHSCREIVNARHPAGRVRERVTPLTTSGTATLNGTTVYFYNDPRNGNCPAHEY
                     IPQ"
BASE COUNT          396 a          686 c          561 g          530 t
ORIGIN      
        1 ctgctttaca tttaacaaat aaaaataatg tcataacgca acatgttata aaaaaccttt
       61 tcatgaaaag gatctccaaa aaaggtggct agagcttagt ctggccacct ttctttattg
      121 aggaatatat tcgtgagcag gacaattacc attccgggga tcattataaa agtaaactgt
      181 tgttccattg agcgttgctg ttcctgacgt tgttaacgga gttacacgtt cacgcactcg
      241 tcctgccggg tgtcgagcat taactatttc acgacagcta tgaacaaccg cacagtcctc
      301 atttcctaca caaaacatat ttccaccacc agataaatct ttaccatttg tcgcattttg
      361 aatattttcc catgcggctt tatccgatgg tgcagaagca ttactaccat ctacggggtg
      421 gcagtttctg gaagcattgt ttcttgagca ggcagcgcca atgctggctt tcatatccgc
      481 aagtccgggc gggggagtaa aatcgcgttg actcgtaaca cagctattac ctacctcacc
      541 ataggtccaa ttgtcctctt gagttaaacc tgttgggtca ataaactggg ctggattatt
      601 aagcacataa ctatagggat tccacccccc caccggccca atcggatcct gggtaatata
      661 cctcccctgc aacggatcat agtaccggtt gcggttatag tacagccccg actcctcatc
      721 atactgctgc cccggcaggc ggtatggctg atacacatga tgcgggttct cctcattaag
      781 ctggttgccc cattcatcat actccccgcg ccacgccgta ttgccgtctt cgctgatgag
      841 cgccagcggc aggccccggt ggtcgcagtg ataaagatga acttttcgcg ccggtgtgta
      901 ttccggctcc acctgtctgg ccagttgctc caccgtcagc ccgcactgcg caagccacgc
      961 ccggctttca ctgctcacgc ggtctgcccg gatttcttcc tccagcctgt ccagcagccg
     1021 caccagttca gccggaaata ccacgccgtg cccgtcctcg ctcccttcct gctgcagctt
     1081 ctccgccagg ctgcggcgct gcgctttttt ccgttcgccg ttctctgtct cgacccggat
     1141 gagcggcgtg aagcttcccg gctcgtatac cgtctggata cgtgtggtgt cagtctgcac
     1201 cgtcgtcagc ctgtcgccgt cccatccgta ccatgtctcc tccggtttac gcgacagcga
     1261 catccacccc gtcaggtcac gctcccgccg ccagacccgt tttgccattc gccgtcccag
     1321 cgggtcacag aggtagcggc tctcgaccag tggctcacca tgctgtatcc gagtataaaa
     1381 caccaggcgg tgctgactgt cgtagtggta gtggtgggtc cgctcgtcgt ccgtccggat
     1441 aacccctgcc cggatgcggt ccgtcttctc cgtcagcctg ccgtactcat cgtagcggta
     1501 caggtagtgc gcgtcttcgg tgatgcggtt atccggccac accgtgagcg tgctgtcagg
     1561 atgcagctcc gggtccggca ggcggttgcc cgccgggtcg gtggcatacg ggatgcggat
     1621 gtccaggtcc ggtgcgaggg tgcgcacgct ctccagcctg cccgtggcac tgtagccgta
     1681 ttcccgcgtc tgtcgcgggc cgctgatgcg caccaggtcg ccgttgtcat tccacccgta
     1741 gtcacggtca tacaccaggc tgttcaggtg ctggctctgt aactggcctg tgggggtgta
     1801 agtgctggtc agttcatatg cattattgcc gaagctgcgc accgtctccc ggtgcaggcg
     1861 gtcgcgcgtg aactccagca gcggcgtccc gcccagcttc atgcccgcca ggtaaccgct
     1921 gccatacgtc agccactcca ccggcggcag gctgtccggc gtgacgcggt ttgccagccc
     1981 ctgttcgttg tatgcgtgct ctgtctcatg ctgccacagc agttcccccg tctccgggtt
     2041 atgcaccgtc tggcgctccc cggccaggcg gcccttatca tcataaccgt aatgcaccgc
     2101 cacctgatgg ccttcgctca ggtggctgat gtctgtcagc cagccgtggc cgtcatactg
     2161 ccactgctct gcc
//
LOCUS       DAZFKF010000276         2162 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFKF010000276 DAZFKF010000000
VERSION     DAZFKF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.279"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>2162
                     /locus_tag="ACHKA1_005448"
     CDS             <1..>2162
                     /locus_tag="ACHKA1_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5682950.1"
                     /translation="VVWPYTNSDVVVDDNGVGFRYSFSAMKDRHTAVEVNYTDPQNGW
                     QTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGS
                     QGLRHTPGDIIEICDNDYAGTLTGGRILSIDAASRTLTLDREVTLPETGTSTVNLING
                     SGKPVRVDITAHPAPDRIQVSVLPDGVATYGVWGLSLPSLRRRLFRCVSIRENTDGTF
                     AITAVQHVPEKEAIVDNGARFEPMSGSLNSVIPPAVQHLTVEVSASDGQYLAQAKWDT
                     PRVVKGVRFSLRLTSGSGENSRLVTSAITADTEHRFSGLPPGEYTLTVRAINSYGQQG
                     EPATTTFRINAPAAPASIELTPGYYQITAVPVLAVYDPTVQYEFWFSEKRITDMAQVE
                     TFAHYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSVFVEASGRASNDAEGYLGLF
                     REKIGKLHLAQGMWELIDNSQLDDEMAEMKTTITETRNEITQTVSKTLEDQSATIQQI
                     QRVQTDTNNDLAALYMLKVQKTKNGIPYVAGIGAGIEDADGRPLSNILLQADRIAMIN
                     PENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPEGRLTARNADISG
                     HISANSGTLNNVVIAENCTINGTLKAENIIGDLVKCAGVAFPVDGSYLANGTRTLTVY
                     DDHSFDRQIIIPPIIYVGSKQESRTSNDIWTECFLHVD"
BASE COUNT          507 a          576 c          672 g          407 t
ORIGIN      
        1 gtggtgtggc cgtacaccaa cagcgatgtg gtggtggatg ataacggcgt ggggttccgc
       61 tacagcttca gtgccatgaa ggaccggcac acggcggtgg aggtgaatta caccgacccg
      121 cagaacggct ggcagacctc cacggaactg gtggaagacc ctgaagccat actgcgctac
      181 ggacgcaacc tgctgaagat ggatgcgttc ggctgtacca gccgcggtca ggcccaccgt
      241 gccggactgt gggtgataaa gaccgaactg ctggaaacgc agacggtgga tttcacgctc
      301 gggtctcagg ggctgcggca cacacccggt gacatcattg aaatctgtga taacgactat
      361 gccgggaccc tgaccggcgg acgcatcctg tccatcgatg ccgccagccg cacactgacg
      421 ctggaccgtg aggtgaccct gccggagaca ggtacatcga cggtgaacct gattaacggc
      481 agcggtaagc cggtgcgcgt ggacatcact gcacaccccg ccccggaccg gatacaggtc
      541 agcgtcctgc ctgatggcgt ggcgacatac ggtgtgtggg gactctccct gccgtcactg
      601 cgtcgtcgcc tgttccgctg tgtttccatc cgggaaaaca cggacggcac ctttgccatc
      661 acggcggtgc agcatgtgcc ggaaaaagaa gctattgtgg ataacggggc ccgctttgag
      721 ccgatgtccg gctcactgaa cagcgtcatc ccgccggcag tgcagcacct cacggtggag
      781 gtgagtgcct cagacggcca gtatctggca caggcgaaat gggacacgcc gcgggtggtg
      841 aagggcgtgc gcttcagtct gcgcctgacc agtggcagtg gtgaaaacag ccgcctggtg
      901 accagcgcca tcactgccga cacggagcac cgtttcagtg gcctgccgcc cggggaatac
      961 accctgacgg tcagggcgat aaacagctac ggccagcagg gcgaacctgc caccactaca
     1021 ttccggatta atgcgcctgc agcaccggcc agcattgagc tgacgccggg gtattatcag
     1081 ataacggcag taccggtgct ggcggtgtat gacccgacgg tacagtatga attctggttc
     1141 tcagaaaaac gcatcacgga catggcacag gtggaaacct ttgcccatta tctggggaca
     1201 ggcagccagt ggagtgtctc cggcccgcac attaagccgg ggaaggattt ctggttttat
     1261 gtgcgcagcg tcaacctggt ggggaaatct gtgtttgtgg aagccagtgg ccgggccagc
     1321 aatgatgcag aagggtatct ggggctgttt cgggaaaaga taggaaaact gcatctggca
     1381 caggggatgt gggagctgat tgataacagc cagcttgatg atgagatggc agagatgaag
     1441 accaccatca cagaaacccg caatgaaatc acacagacgg tcagtaaaac cctggaagac
     1501 cagagtgcca ccatacagca gatacagcgg gtgcagacag acacaaataa cgacctggct
     1561 gcgctgtaca tgctgaaggt gcagaaaacg aaaaacggca ttccgtatgt tgccggtata
     1621 ggtgcgggga ttgaggatgc tgatggccgg cccctgagca atatactgct gcaggcggac
     1681 cgtatcgcga tgattaaccc ggagaacggc aacaccacgc cgctgtttgt ggcgcagggg
     1741 aatcagctgt tcatgaacga tgtgttcctg aagcgactgt ttgcagtgag catcacctcg
     1801 tccggcaatc ccccgacgtt ctccctgacg ccggagggca ggctgacggc ccgcaatgct
     1861 gacatcagcg gacatatcag tgcgaactcg ggcacgctca ataatgtcgt gatagcggag
     1921 aactgtacga taaatggcac gctgaaagcg gagaacatta ttggtgatct tgtgaaatgt
     1981 gcaggggtgg cttttccggt ggatggtagt taccttgcga acggtacacg aacgctgacg
     2041 gtgtatgacg atcacagctt tgaccggcag attataatcc cgccgataat ctatgtcggg
     2101 tcaaaacagg aatcccgtac cagtaatgac atctggacag agtgcttcct gcatgttgat
     2161 ca
//
LOCUS       DAZFKF010000277         2161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFKF010000277 DAZFKF010000000
VERSION     DAZFKF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.280"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            11..>2161
                     /locus_tag="ACHKA1_005449"
     CDS             11..>2161
                     /locus_tag="ACHKA1_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO5682951.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQQSA
                     ATSASTATTKASEAASSARDASASKEAAKSSETSAASSASSSASSATAAGNSAKAAKT
                     SETNAKSSETAAEQSASAAADSKTAAASSASAASTSAGQASASATAAGKSSESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIVQLSSATNSTSETLAATPKAVKTANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGA"
BASE COUNT          605 a          590 c          621 g          345 t
ORIGIN      
        1 tggggtgaat atggcagtaa agatttcagg tgtactgaaa gacggcacag gaaaaccggt
       61 acagaactgc acaatccagc tgaaagcaaa acgtaacagc accacggtgg tggtgaacac
      121 gctggcctca gaaaatccgg atgaagccgg gcgttacagc atggacgttg agtacggtca
      181 gtacagcgtt attctgttgg tggaagggtt cccgccgtca catgccggga ccatcaccgt
      241 gtatgaagat tctcaacccg gtacgctgaa tgattttctt ggtgccatga ctgaggatga
      301 tgtccgtccg gaggcactgc gccgttttga gctgatggtg gaagaggtgg cgcgtaacgc
      361 gtccgcggtg gcacagaaca cggcagccgc gaagaagtca gccagcgatg ccagcacatc
      421 agcccgtgag gcggcaaccc atgcgactga tgctgcggac tcagcacgcg cagccagcac
      481 gtcagccgga caggccgcgt cgtcggctca gtcagcgtct tccagcgcag gaacggcatc
      541 aacaaaggct actgaagcat caaaaagtgc tgccgctgca gagtcctcaa aaagcgcggc
      601 ggctaccagt gccggtgcgg cgaaaacgtc agaaacgaat gcggcagcgt cacaacaatc
      661 agccgccact tctgcatcca ccgcgaccac gaaagcgtca gaagctgcct cctcagccag
      721 ggatgcgtcg gcttcaaaag aggcggcaaa atcatcagaa acgagtgcag cctcgagcgc
      781 cagtagttcc gcttcctcgg caacggcggc aggcaattcc gcgaaagcgg caaaaacgtc
      841 cgagacgaac gctaagtctt ctgaaacggc agcggaacag agtgcctccg cagcagcaga
      901 ctcaaaaaca gcggctgcat catctgccag cgccgcgtca acaagtgccg ggcaggcctc
      961 agccagtgcc accgctgccg gaaagtcgtc agaaagtgcc gcatcgtctg cttcaacagc
     1021 cacaacgaag gctggcgaag ccactgaaca ggccagcgca gcagcgagtt ctgcttccgc
     1081 agcgaagaca tccgaaacga acgcgaaagc gtcggaaacc agcgcagaat cctcaaaaac
     1141 ggctgccgca tcgtcagcca gttcggcggc gtcatcggca tcatctgcgt ctgcttcaaa
     1201 agatgaggcg accagacaag cgtcagcagc gaagagcagc gccacgacgg catccacgaa
     1261 ggcgacagag gcagctggta gtgcgacggc agcagctcag agcaaaagta cggcggaatc
     1321 tgcagcaacg cgcgctgaga cagcggcaaa acgggcagag gatattgcat ccgccgtggc
     1381 gcttgaggat gcgagcacga cgaaaaaggg gatagtacag ctcagcagtg cgaccaacag
     1441 cacttccgag tcactggcgg caacgccaaa agcagttaag gccgcgtatg acctggctaa
     1501 cgggaaatac acagcacagg atgcaacgac agcacagaaa gggatagtcc aactaagcag
     1561 cgcgaccaac agcacgtctg aaacgctggc ggcaacgcca aaggcagtaa aaacagccaa
     1621 tgacaatgct gagaaacgtc tgcagaaaga tcagaacggt gcggatatcc ctggcaaaga
     1681 cacctttacg aaaaatattg gtgcctgccg tgccttcggt gggtcagtaa gcacaacaac
     1741 aggaaattgg acgactgcac agtttatcga gtggctggat tctcagggag catttaacca
     1801 tccatactgg atgtgcaagg gttcctggtc ttatggcaat aataaaatca ttactgatac
     1861 tggctgcggt aatattcatc tcgccggagc tgtcattgaa gtaatgggga taaagtcagc
     1921 gatgacgatc cgcattacca ctccgaccac atccagcggt ggcggaacaa ccaacgccca
     1981 gtttacctat attaatcacg gaacagatta ttcacctggc tggcgaaggg actataactc
     2041 cagaaataag ccaacggcat cagagatcgg ggcgctaccg tcaggtggaa cagcagtatc
     2101 atcagttaat ctggcttcaa aaggtcgggt agccgcgctg acagacaata cgcagggggc
     2161 a
//
LOCUS       DAZFKF010000278         2154 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFKF010000278 DAZFKF010000000
VERSION     DAZFKF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.281"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..117)
                     /locus_tag="ACHKA1_005450"
     CDS             complement(<1..117)
                     /locus_tag="ACHKA1_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682952.1"
                     /translation="MLAISSNISKMVIFIFAIIIVVFLCVTTYLYLHKDESLV"
     gene            complement(98..>2154)
                     /locus_tag="ACHKA1_005451"
     CDS             complement(98..>2154)
                     /locus_tag="ACHKA1_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5682953.1"
                     /translation="EPAEQWQYDGHGWLTDISHLSEGHQVAVHYGYDDKGRLAGERQT
                     VHNPETGELLWQHETEHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGGTPLVE
                     YTRDRLHRETVRSFGSMAGSNAAYELTSTYTPTGQLQSQHLNSLVYDRDYGWSDNGDL
                     VRISAPRQTREYGYSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPELHPDSTLTA
                     WPDNRIAEDAHYVYRHDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFYTRI
                     QHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEVTWYDWDGDRLTTVQT
                     DTTRIQTVYEPGSFTPLIRVETENGEREKVQRRSLAEKLQQEGSEDGHGVVFPAELVR
                     LLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHLYHCDHRGLPL
                     ALISEDGNTAWRGEYDEWGNQLNEENPHHLHQPYRLPGQQYDEESGLYYNRNRYYNPL
                     LGRYITQDPIGLAGGWSLYAYPLNPVNGIDPLGLSPADVALMRKKEQLNHQRAWDILS
                     DTYDDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNMFGMY
                     GRKVKLSHSEMIEDNKKDLAVNEHGLTCPLTQDCSNRCIDYINPEHKKTIKALQDAGY
                     LK"
BASE COUNT          402 a          683 c          520 g          549 t
ORIGIN      
        1 tacaagagat tcatctttgt gtaaataaag ataagtagtc acgcataaaa aaacaacgat
       61 tataatagca aaaataaata ttaccatctt tgagatgtta cttgagatag ccagcatctt
      121 gtaaagcctt tatcgttttt ttatgctctg gattaatata atcaatacat ctatttgagc
      181 aatcttgtgt taatggacat gtcaacccat gttcatttac agccaagtct tttttattat
      241 cttcaatcat ctcagaatgg gataacttta cttttctgcc gtacataccg aacatattta
      301 acccgtaatc tctaatctct ttttcataac ccagcccttt cgctgatcgg ctaacaccag
      361 cgtcatttaa tttagacact cgacaaaatg ccatacaatg gaaaaattga tcagtcccac
      421 ctaaatttaa tctcttcata tcatcataag tatcagataa tatatcccat gctctttgat
      481 ggtttagttg ttcttttttt cgcattaacg ctacatctgc aggacttaac cctaatggat
      541 caataccatt caccggattc agcggatacg catacagact ccatcccccc gccagcccaa
      601 tcgggtcctg agtaatatat cgtcccagca acggattgta gtaccggttc cggttataat
      661 acagccccga ctcctcatca tactgctgcc ccggcagccg gtacggctgg tgcaggtgat
      721 gcgggttctc ctcattcagc tggttgcccc attcatcata ctccccgcgc cacgccgtat
      781 tgccgtcttc gctgatgagc gccagcggca ggccccggtg gtcgcagtga taaagatgaa
      841 cttttcgcgc cggtgtgtat tccggctcca cctgtctggc cagttgctcc accgtcagcc
      901 cgcactgcgc aagccacgcc cggctttcac tgctcacgcg gtctgcccgg atttcttcct
      961 ccagcctgtc cagcagccgc accagttcag ccggaaatac cacgccgtgc ccgtcctcgc
     1021 tcccttcctg ctgcagcttc tccgccaggc tgcggcgctg cactttctcc cgctcaccgt
     1081 tctctgtctc gacccggatg agcggcgtga agcttcccgg ctcgtatacc gtctggatac
     1141 gtgtggtgtc agtctgcacc gtcgtcagcc tgtctccgtc ccagtcatac cacgtcacct
     1201 ccggtttacg cgacagcgac atccaccccg tcaggtcacg ctcccgccgc cagacccgtt
     1261 ttgccattcg ccgtcccagc gggtcgtaga ggtagcggct ctcgaccagt ggctcgccat
     1321 gctgtatccg cgtgtagaac accaggcggt gctggctgtc gtagtggtag tggtgggtcc
     1381 gctcgtcgtc cgtccgtatc acacccgccg ggatgcggtc cgtcttctcc gtcagcctgc
     1441 cgtattcatc gtgtcggtag acatagtgcg catcctccgc gatgcggtta tccggccacg
     1501 ccgtgagcgt gctgtccggg tgtagctccg ggtccggcag ccggtttccc gccgggtcgg
     1561 tcgcatacgg gatgcggatg tccaggtccg gtgcgagggt gcgcacgctc tccagcctgc
     1621 ccgtggcgct gtagccgtat tcccgcgtct gccggggggc gctgatgcgc accaggtcgc
     1681 cgttgtcact ccacccgtag tcacggtcat ataccaggct gttcaggtgc tggctctgta
     1741 actggcctgt gggggtgtaa gtgctggtca gttcatatgc ggcattactg cctgccatgc
     1801 tgccgaagct gcgcaccgtc tcacggtgca gcctgtcccg cgtatactcg accagcggcg
     1861 tcccgcccag cttcatgccc gccaggtaac cgctaccata cgtcagccac tccaccggcg
     1921 gcaggctgtc cggcgtgacg cggtttgcca gcccctgttc gttgtatgcg tgctctgtct
     1981 catgctgcca cagcagttcc cccgtctccg ggttatgcac cgtctggcgc tccccggcca
     2041 ggcggccctt atcatcataa ccgtaatgca ccgccacctg atggccttcg ctcaggtggc
     2101 tgatgtctgt cagccagccg tggccgtcat actgccactg ctctgccggt tcac
//
LOCUS       DAZFKF010000279         2096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFKF010000279 DAZFKF010000000
VERSION     DAZFKF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.282"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1189
                     /gene="rfaL"
                     /locus_tag="ACHKA1_005452"
     CDS             <1..1189
                     /gene="rfaL"
                     /locus_tag="ACHKA1_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO5682954.1"
                     /translation="SLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVILMTITTI
                     VYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAIIENTLLCT
                     IAIPVILRDEKRENIEKIVFFSFISALSLRCFSELIAYYKDYQQGIMPFADYRHRSIS
                     DSMVFLFPALLNLWLIKSAKYRISFVVLSFIFIFLILGTLSRGAWLSVLVIGLIWILM
                     FKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGSALDLILE
                     NPVIGYGYGNVAYKDVYNKRVVDYPEWTFRQSIGPHNFALFIWFGTGLLGLVSLMMLY
                     CAILKECIKNGVQNKYRSPCNAYYIILLSFIGYFVIRGNVEQIEPNLLGVYAGLLLAM
                     KNK"
     gene            complement(1225..>2096)
                     /locus_tag="ACHKA1_005453"
     CDS             complement(1225..>2096)
                     /locus_tag="ACHKA1_005453"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO5682955.1"
                     /translation="AHDFPITKESVIVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPK
                     LLEQNVKMIVPSLYLKKYYQSYLANADIEIVPNGIDLETYQSTFQPITRSELNISPEE
                     KIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREI
                     AKRLKDRCIMLGSIPPEKMHCYYPLADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTR
                     GGMTEFVKENTTGFHLKEPMTADSISSDILKTLANPELTAVAKQGQDFVFDHYSWDGV
                     TQRFEEVIHNWFE"
BASE COUNT          636 a          392 c          362 g          706 t
ORIGIN      
        1 ctctctcgag aaaaaaaact ggatagcgta ctggaacaga gctctcgtat tcttatttat
       61 taccacctat tttttgggtg ggattacaag gtataaacat cttattgtta ttcttatgac
      121 aataacgaca atcgtctatc tctgcaaacg gccaaaacac tatctatcac tatttaaaac
      181 atttcttttt ggtagtgttg ccatattaac tattgctgca ttgctgtcac ttcttcaatc
      241 tccagatgca ggtgctagca tgaaggaaat tttcaaagct atcattgaga atactttact
      301 atgcacaata gcaataccgg tcatattgag agacgaaaaa agagaaaata tcgaaaaaat
      361 cgttttcttc tcatttatta gtgcgttgag cttacgctgt ttttctgaat tgattgccta
      421 ttataaggac tatcaacaag ggataatgcc attcgcagat tatagacacc gtagcatttc
      481 tgactcgatg gtctttttat tccctgcatt gttaaatctc tggcttatta aatcagcaaa
      541 ataccgcatt tcttttgtgg ttctaagctt tatttttatt tttctgatat taggaacttt
      601 atccagaggg gcctggcttt ccgtgttagt cattggatta atatggattc tgatgtttaa
      661 acaatggaag ttactattag taggagtaat ggtttccatc attgcattgt cggttatttt
      721 cacacataag gagatgaccg caaagctaac gtataaactt caacaaacta atagttctta
      781 tcgctatgca aatggtactc aaggcagcgc actcgatcta atattagaaa atcctgttat
      841 tggttatggt tacggtaacg ttgcatataa agatgtctat aataaacgtg tcgttgatta
      901 tccagaatgg acctttagac aatcaatagg gccacataat tttgcgctat tcatctggtt
      961 tggcactggt ttattagggc tggtaagtct tatgatgcta tactgtgcaa tattgaaaga
     1021 gtgtataaaa aatggcgtcc agaataaata ccgctcacca tgtaatgcat attatataat
     1081 cttactatct tttataggtt attttgttat ccgtggaaac gtagaacaaa ttgaaccaaa
     1141 tttattaggc gtttacgccg gcttattatt agcgatgaaa aataagtaaa aaataaaaaa
     1201 ggctgcataa tgcggccttt ttatttattc aaaccaatta tgaataacct cttcgaatct
     1261 ttgagttacg ccatcccaac tgtagtgatc aaacacaaaa tcctgacctt gctttgcaac
     1321 tgctgttaat tcaggattcg ctaacgtttt taaaatatcg ctgcttatgg agtctgcagt
     1381 cataggttct tttaaatgga atcccgtagt attttctttg acaaattccg tcatcccccc
     1441 acgcgtactc actaaaacag gcttaccagc acccatagct tcaattgcca ccatgcaaaa
     1501 tggttcctgg aactgggaag gaatgacgac taaatctgcc agtgggtaat aacaatgcat
     1561 cttttcaggc ggtatgcttc ctaacataat gcaacgatcc ttcaaacgtt ttgcaatttc
     1621 cctgacattg cgttgatatg cacctttgtc acttttactc atttctgtat aatcaccaat
     1681 cacaactaat tttaaatttt tgtgagcagc ggccaatttt tcgaatgatt gcatcaacag
     1741 caatatgcct ttgtcaggga cgatacgccc agcataaaaa ataattttct cttcagggga
     1801 gatattaagt tcgcttctag tgatcggctg aaaagtagat tgataggttt ctaaatcaat
     1861 tccatttggt acaatctcaa tatctgcatt tgctaaataa gattggtaat actttttcaa
     1921 gtataaacta ggcactatca ttttaacatt ttgctcaagc aattttggtt caaatgcatt
     1981 atgcatatgt ataaccactc ttgcctgagg agctcgctta cgaatttgag tgtatagctt
     2041 catactatta tgaactacaa taacactttc tttcgttata ggaaaatcat gtgcaa
//
LOCUS       DAZFKF010000280         2067 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFKF010000280 DAZFKF010000000
VERSION     DAZFKF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.283"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1279
                     /gene="sctN"
                     /locus_tag="ACHKA1_005454"
     CDS             <1..1279
                     /gene="sctN"
                     /locus_tag="ACHKA1_005454"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HFO5682956.1"
                     /translation="LLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASWQANERIARA
                     QVVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQIMERFV
                     PDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFASAGCG
                     KTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKQSVNAAKCVLVYATSDFSSVD
                     RCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYPASVFD
                     SLPRLLERPGPTLKGSITAFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLAGQGHY
                     PAIDVLKSVSRVFGQVTDEKHRDNAARVRKILTTLEDLQVFIDLGEYRAGQNAENDFA
                     MNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            1269..1727
                     /locus_tag="ACHKA1_005455"
     CDS             1269..1727
                     /locus_tag="ACHKA1_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFO5682957.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVAVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYERLKTRIKKQLLDQMLYQDELEQEEKYNGRSQEN"
     gene            1705..1935
                     /locus_tag="ACHKA1_005456"
                     /pseudo
     CDS             1705..1935
                     /locus_tag="ACHKA1_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
BASE COUNT          685 a          377 c          479 g          526 t
ORIGIN      
        1 attattaaat aaatacagct acttacatag cataaacggc tctctgattg aagcagaact
       61 tgacgacgtt tctgtgggtg aagtatgcga aatttatgcg agctggcaag caaatgaacg
      121 tatagccagg gctcaggtcg ttggctttcg caatggaaag acattattaa accttatagg
      181 cagctcagtg gggcttacac gtactgcagt tttgaaacca acgggagagc agttaaccat
      241 acagatcagt gatgcctttc ttggttcggt tttgaatgcc tctggtcaaa ttatggaaag
      301 gtttgtcccg gacactccag gcgatagagg aaatttacgc ctgattgatg aactgccacc
      361 gtcgtatcag gaacgtcgag ttattaatac accactagag acacgcataa gagtcattga
      421 tggcgtactg acatgtggca ttggtcaacg tgttgggatt ttcgcatcgg ctggctgtgg
      481 caaaacggtt ttaatgcata tgctcgtaaa caatactgaa gctgatgtgt ttgttattgg
      541 cttgattggc gaacgtggta gggaagttac ggaatgcgcg gaatcgctga aacaatcagt
      601 aaatgcggca aaatgcgtat tggtttatgc cacctctgat ttttcgtcag tagatcggtg
      661 taatgccgcc ctgatggcga caaccgtggc agagtatttt agagatcgag gaaagagagt
      721 tgtacttttt attgactcaa tgacgcggta tgcacgagca ctacgtgata tgaaactggc
      781 agcgggtgaa ccacctgcca gacgtggtta tcctgcttct gtttttgaca gtttgcctcg
      841 cttactggag aggccgggac caacactaaa aggaagtatc accgcgtttt ataccgtgtt
      901 actggaaggg gaagatgaat cagacccact gggtgatgaa attcgctcaa tccttgatgg
      961 ccatatctat ctaagccgca aactcgcggg tcaaggtcac tatccggcaa tagatgttct
     1021 aaaaagtgtg agtcgagtat ttggtcaggt cactgatgaa aaacacagag ataatgctgc
     1081 acgggtaagg aagatcctca caactttgga agatctacag gtttttattg acttagggga
     1141 atatcgcgct gggcaaaatg ctgaaaatga ttttgctatg aatgcacggc ctaaactcac
     1201 aaattggttg aaacagtcag ttaatgaaaa aatgccaatg agtgaaactc taaaagagct
     1261 tgaacgaatt gttaagtaaa gttaatcgac ttataagaag aacagcgcag agcctggctg
     1321 catgtgaagc atcattacaa aagttaaatg cagaaaaaga aaagttagcc gaaaaggaac
     1381 gcctgtatga tatgcaactt aaaaacctgc agtcactgtt agatatgaaa gagttactcg
     1441 gcgaagttgt atttcgccag gatatatttt actcgttacg taaagttgca gtcattcagc
     1501 agcagatcgc tgagataaat cttgaaaagc aaaaaatagc agagcggaga aaaatactta
     1561 acaaggaaat agttcaacag caagcacaaa gaaaacactg gtggctaaaa ggagaaaaat
     1621 acgaacgact aaaaacgcgc atcaaaaaac aacttttaga tcagatgctt tatcaagacg
     1681 agcttgaaca ggaggaaaaa tataatggac gaagtcaaga aaattgaaca cggtaaaatt
     1741 ttaaacacag acacacccga taattctgct tcggatttag acagtttctt aaagaaaaac
     1801 aaaaaaaata aagaaatgag tgagattgtc gctggaacca ttctccagtt attacgtcag
     1861 actcaggata gccaattttc tacgttgggg aaaaaaaatg ggcaaatgaa gtctattgac
     1921 aacaaatcat ctctccaggt tgacaggaat ccctctcttt ctttgcaaac aaatggaaca
     1981 gagcaatttt ctacaaagaa aattctcaat atgacttctc gaggtcaggg tattagttct
     2041 gaactatctg ataacacgat aaaaatt
//
LOCUS       DAZFKF010000281         2066 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFKF010000281 DAZFKF010000000
VERSION     DAZFKF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.284"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(254..349)
                     /gene="dqlB"
                     /locus_tag="ACHKA1_005457"
     CDS             complement(254..349)
                     /gene="dqlB"
                     /locus_tag="ACHKA1_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFO5682958.1"
                     /translation="MINLLIIVLRAVVAVANAVIAILELIRELID"
     gene            848..>2066
                     /locus_tag="ACHKA1_005458"
     CDS             848..>2066
                     /locus_tag="ACHKA1_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO5682959.1"
                     /translation="MSYQHSPTTEDPMNIWYIVGTICLLFAFLIWLFLPDIVFATCLI
                     LHTMWGMTDWGPFHHFAAPRYNLLANTANNAATITFSQWLDVMSQTVGILWLILLPMT
                     LGFLWMWFQHPAQPRFTRRPLNIHTLPHLFSSLSPAIAPVLADGDSNRLFHGQKRPER
                     RVALTPEVFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTGE"
BASE COUNT          500 a          591 c          490 g          485 t
ORIGIN      
        1 atgcgtgaac aaatgagcgg gataatccgg aagactggca cagacgaaat actcttccgt
       61 ctgagtaagc accatcattt atatgagaaa aagaaaacaa aagagggatg acaatcccat
      121 gatatgggaa aaacttgcca tgcttactgc aggtgcattt ttgatactgg cagtcctgtg
      181 ggggtaataa tatcctgttt attaaagggc agaaggcggt gcccctctgc ccttaagtac
      241 gtttccggga ttatcagtca ataagttccc ggatcagttc caggattgct atcactgcgt
      301 tagccacagc aacaaccgcc ctgagaacga tgattaacag gttaatcatc cgtttctccg
      361 tcaatggagg aaatcactgt tgtttactct tacattgcct gtcacaacag ctctttcatt
      421 gttagatgta aagtgatttc tctccagcca gagcactgaa cacagcacca cctgttcgat
      481 cagccaggac ttaaaatctc gcgttcatcg tcccattgga gatcacggca gaagcatcac
      541 cagacagagc taacggagcg attatagtca tttttctgat tgcaaaaaag agaagcgcag
      601 cctactatga tggtgttaga tgtagagtga tttccctttc ggtcagagtc accaactccg
      661 ccccggaata gcaaaaaaac cgacctcacc agtcggtttt tttgtgcctg tctctcactg
      721 ccttctgctg ttcctgtgaa aataccctgg ttatttcatc cctgcccggt cattaccggc
      781 gattgttcgc attaattcca ctcagcctca tcccgaaatt tatccattta tcgatggagg
      841 aatactcgtg tcttaccaac actcacccac aactgaagac ccgatgaata tatggtatat
      901 cgttggcacc atatgcctgc tctttgcgtt tctcatctgg ctctttttac ccgacatcgt
      961 ctttgccacc tgcctgatcc tgcacacaat gtgggggatg actgactggg ggccttttca
     1021 ccattttgcc gccccccgtt ataacctgct ggccaacacc gccaacaacg ctgcaacgat
     1081 cactttcagc cagtggctgg atgtcatgtc acagaccgtg gggatcctct ggctgattct
     1141 gcttcccatg acgctcggct ttctgtggat gtggtttcag catccggccc agccccgctt
     1201 cacccgcaga ccactgaata tccacacgct gccacacctg ttttcatcac tgtcaccggc
     1261 gatcgccccc gttctggctg acggagacag caacagactc ttccacggac aaaaacgtcc
     1321 cgagcgccgt gtcgcactaa cccccgaagt ctttgtggag cagaacaacc ttatccgcaa
     1381 catgcagctc gatgttgcgt ccacacgcca gtgcttcatg gcgcaactgg ggcaaccact
     1441 gaccagctgg aaagacatgg ccccacatga aaaagcgctc tttgccatct ttggcctgca
     1501 gttcttcctc ggtgaccgga aagcagcggt agccctgatg aacaacctca atctctcctg
     1561 caggctcaaa agtaaacggg accaggggcg tttcagcacg ccggtgtatt cactggccag
     1621 aaacgccttt atccgggtca tcaaaacaga aggcgcacaa cagtggctca ggcagcaccg
     1681 ttacgttcgc agcggtctgg tctggcttta cgcacatgat ttgcgcctga cgcctccgaa
     1741 ctggctgtgg ctcaagggcg ttgaccggac gctcttctat gcccttcacc gggcaaacac
     1801 cacaaaaggc ttcatcgaag gggccggcgt ggtggccgtt gcccgggcag aaaatgaagc
     1861 cagccgtctc ggcctcccct gcccggaacc ctgtgtggaa gaggccattg aagggcttcg
     1921 acgggacatg ctggggctgg gcctgatatg ggacgaacct cagccagatc gcgatcgtaa
     1981 acgtcagatc aggacccgct ggtcactcac ggatgacgtg ataccccgcc gccatgataa
     2041 cgacgaagac actgacactg gtgaaa
//
LOCUS       DAZFKF010000282         2055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFKF010000282 DAZFKF010000000
VERSION     DAZFKF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.285"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            381..971
                     /locus_tag="ACHKA1_005459"
     CDS             381..971
                     /locus_tag="ACHKA1_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5682960.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
     gene            complement(1069..1644)
                     /locus_tag="ACHKA1_005460"
     CDS             complement(1069..1644)
                     /locus_tag="ACHKA1_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5682961.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAADLEIATEEE
                     ISLLEAWKKYRVLLNRVDTSTSQDIEWPALP"
     gene            complement(1644..>2055)
                     /locus_tag="ACHKA1_005461"
     CDS             complement(1644..>2055)
                     /locus_tag="ACHKA1_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682962.1"
                     /translation="SSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTHSV
                     TGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHS
                     VAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT          571 a          488 c          424 g          572 t
ORIGIN      
        1 tacccccccc tgaaaaataa tttttcaatg cattaaaaaa atatcaacaa ctacgttatt
       61 ccgcaacaac ctactttttt taccattttt ccactttaac aacattttgc tccacttttc
      121 cacgaccaaa caacttaaaa tctggttgaa ataacataca acactattat tcgtcttcct
      181 cgagtccgcc cggagttcga aaaacaaacc gagttaaagc catttttcaa aaaatcgatt
      241 tttggttaca ccaaaattac gggactgcat ccgaattcgt ttattttcga acgtatacat
      301 acaaatatgt acaataataa gcgaaataat ttttggatat gcattatgat atgtacacca
      361 atttcgtata gagtctcact atgtctcgaa tttttgctta ctgtcggata tcaacgctgg
      421 atcagaccac cgaaaatcaa cgccgggaaa tcgaaagtgc aggttttaaa atcaaacctc
      481 agcaaataat cgaagaacac attagcggct cagcagcaac cagtgagcgt cctggtttta
      541 accggttgct tgctcgtctg aaatgtggtg atcaattgat tgtgacaaaa ctggatcgcc
      601 ttggttgtaa tgcaatggat atcaggaaaa cagtggaaca actgaccgaa acaggtatca
      661 gagtgcattg cttagcattg gggggcattg acctgaccag tccaacaggg aaaatgatga
      721 tgcaagtaat ttcagcagtc gctgaatttg aacgagacct tttacttgaa cgcactcatt
      781 ccgggatagt aagagcccgg ggcgcaggga aacgttttgg tcgaccacct gtgttaaatg
      841 aagaacagaa acaggtggta ttcgaacgaa ttaagtcagg tgtaagtata agtgccattg
      901 cccgggaatt caaaacctcg cggcaaacca ttttaagagc caaagcaaaa cttcagacac
      961 ctgacatata aaaaataatc ttggtgtgag atgctttacg tcttccaagc ccccttcctt
     1021 gccgtaaatg gaaagataca tctaattata gaatttatat gttttaccct acggcagtgc
     1081 tggccattca atatcctgtg aagttgacgt atcaacacgg ttcagcaata cccgatactt
     1141 tttccatgct tccagcaacg agatttcttc ctccgttgca atttccagat ctgcagcatc
     1201 ctgaagcggc gcaatatgct cactggctac ctgcatcagg ctgttttttg tttcttccgc
     1261 ctctcggatc cggaacagtt tttctgcttc tgcatctttc acccaggctg tgccgttcca
     1321 cttctgaaac tccccttccg gcgataacca ggtaacattt tccggtaacg gaccgagttc
     1381 agaaataaat aacgcgtccc ctgacgctac gtcataaacc gttttacccc gatggtcttc
     1441 aacgagatgc cacgatgcct catcactgtt gaaaacagcc acaaagccag caggaatatc
     1501 tggtggtgca atatcggtac tgtttgctgg cagacctgta tgaggcggaa tatatgcgtc
     1561 accttcacca ataaattcat tagttccggc cagcagatta taaattttta tggtccgtgg
     1621 ttgttcactc attctgaatg ccattatgca agcctcacaa tgtagttaaa tgcgatgttt
     1681 ttgacggtgt tttccgcgtt accagcagcg ttaacggtga tagtgtgtcc atgtgagcca
     1741 atcgcaacag agtgcgtatg agcaccaata ccgacagtat gtgcgtgagc acctgcagat
     1801 gcagcggtgc cgctgacact atgagtgtgt gctcctgcag cacttgtatt cacactggcc
     1861 gcagacacta ccttatgtac tgacccattt tgtgaccact ggctgactgc tgatgcgcct
     1921 gtgacactat gagtatggtt tccggcactt gcggctgtac cgctcacact gtgcgtatgc
     1981 gccccggtgt tattcgtgga tttagtgccg taatcaaacg acgatgtggt tttcgtcccc
     2041 aaatccgtac tggat
//
LOCUS       DAZFKF010000283         2007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFKF010000283 DAZFKF010000000
VERSION     DAZFKF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.286"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..144
                     /locus_tag="ACHKA1_005462"
     CDS             <1..144
                     /locus_tag="ACHKA1_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO5682963.1"
                     /translation="LFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGS
                     GLS"
     gene            154..1569
                     /locus_tag="ACHKA1_005463"
                     /pseudo
     CDS             154..1569
                     /locus_tag="ACHKA1_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1573..>2007
                     /locus_tag="ACHKA1_005464"
     CDS             1573..>2007
                     /locus_tag="ACHKA1_005464"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO5682964.1"
                     /translation="MIIGNGPVPDNPFGHAAAANRYGLMSSGTGDEMGASVSDYFKKI
                     QSRRDTWIWIIDTTDEEEQCMMNKAIDLNKKLKTINLGPLPVLNNCFSRTNMIFEACG
                     FNNPSMNTNAPISLQVLGELYGYQRYIMPKGPFTGFPFEFQEV"
BASE COUNT          503 a          502 c          625 g          377 t
ORIGIN      
        1 ttattttcag ggatacggca cattgctatt aatattttga cgaatgataa ggtattcaag
       61 gcagggttaa gacgtaagat gcgaaaagca gccatggaca gaaactatct ggcgtcagtc
      121 cttgcgggga gcgggctttc gtaatcttgc cctgggctgg cgacccgtca ggagccggac
      181 ggtctgccgc cggtggagtg gctgacgtat ggcagcggtt atcttgcggg gatgaagctg
      241 ggcggaacgc cgctggtcga gtacacgcgg gaccggctgc accgtgagac ggcccgcagc
      301 ttcggcgggg aggcatatga acttgccacc gcctggaata ccagcggcca gctccagagc
      361 aggcacctga accttccgca gcttgaccgt gactacgact ggaacgacaa cggacagcta
      421 atccgcatca gcggcccgca ggagagccgg gagtaccgtt acagtgacac gggaaggctg
      481 acgggcgtcc acaccacggc ggcgaacctg gatatcgata tcccgtatgc aacagacccg
      541 gcgggaaacc ggctgccgga cccggaactg cacccggaca gtaccttcac ggcgtggccg
      601 gataaccgca tcgcggaaga tgcgcactat gtctatcgct acgatgaata cggcaggctg
      661 gcggagaaga cggaccgtat cccggaaggg gttatccgga tgcacgacga gcgcacccac
      721 cactatcact acgacagcca gcaccggctg gtgttctaca cgcggataca gcatggcgag
      781 ccactggtcg agagccgcta cctctacgac ccgctgggac ggcgaatggc gaaacgggtg
      841 tggcgtcgcg ggcgtgacct gacgggatgg atgtcgctgt cgcgtaaacc ggaggagaca
      901 tggtacgggt gggacggcga caggctgacg acggtgcaga ctgacaccac acgtatccag
      961 acggtatacg agccgggaag cttcacgccg ctcatccggg tcgagacaga gaacggcgag
     1021 cgggagaaag cgcagcgccg cagcctggcg gagaagatgc agcaggaagg gagcgaggac
     1081 gggcacggcg tggtatttcc ggctgaactg gtgcggctgc tggacagact ggaggaagaa
     1141 atccgggcag accgcgtgag cagtgaaagc cgggcgtggc ttgcgcagtg cgggctgacg
     1201 gtggagcaac tggccagaca ggtggagccg gaatacacac cggcgcgaaa agttcatttt
     1261 taccactgcg accaccgggg cctgccgctg gcgctcatca gcgaagacgg caatactgcg
     1321 tgaagcgggg agtatgatga gtggggaaac ctgctgaatg aggaaaaccc gcatcacctg
     1381 caccagccgt accgtctgcc agggcagcaa cacgatgagg agtcggggct gtattataac
     1441 cgtcaccggt actacgatcc gttgcagggg cggtatatca tccaggaccc gattgggctg
     1501 aaggggggat ggaatctgta ccaatatccg ctgaatccag tgcaacacgt tgatccatta
     1561 gggcttagta ctatgataat aggtaacggc cccgttcctg ataatccttt tgggcatgct
     1621 gcggcggcaa atcgatatgg attaatgagt tcgggaactg gggatgagat gggagcctct
     1681 gtctctgatt attttaaaaa gatacaatcc cgcagagata cctggatttg gataatagat
     1741 acaacagatg aagaagaaca atgtatgatg aataaggcaa tagaccttaa taaaaaacta
     1801 aaaactatta acctcgggcc tcttcctgtt ttaaataatt gttttagccg aacgaacatg
     1861 atttttgagg cgtgcgggtt taacaacccc tctatgaata caaatgcgcc tataagccta
     1921 caagtgctcg gtgagcttta tggatatcaa agatatatta tgcctaaagg tcctttcact
     1981 ggattcccat ttgaatttca agaggta
//
LOCUS       DAZFKF010000284         1933 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFKF010000284 DAZFKF010000000
VERSION     DAZFKF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.287"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(809..1546)
                     /locus_tag="ACHKA1_005465"
     CDS             complement(809..1546)
                     /locus_tag="ACHKA1_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5682965.1"
                     /translation="MIVCYVILVNRPFETEIAGKKIFFQKNDVLLTDNISQSFFLRHP
                     GNVTELRIDECIVRKYMESADRIQHSDSAVSVPFIKTTFSHIALLHNVIQNLSLQKTL
                     PQQFSEQMFFACLSIFSTHRGFMFLLFRCINSVALKVRYIICSSPSRVWRVKDISNSL
                     YMSESLLKRKLQEEKVSFSQILLDIRMHNARQLISSKLSVNQIAIRCGYASTPYFISV
                     FREYFGITPHQMMINQSGFIEGRIAIK"
BASE COUNT          602 a          392 c          385 g          554 t
ORIGIN      
        1 acctgtccat acttaagatt tttcctgtgg aaaacaaaca ttaatatgtt ttatgtattt
       61 cgcatggtga tgatttatac acctgcactg aatttttctg tatttccaga actgaatcga
      121 taaacagaat tggtaataat gaaagttaat cacataaaaa agaggttacc gccttataat
      181 gataagagtt cattatccat tccatcccga caaaacctgc ctgtgtataa aaaacaagaa
      241 ccatgtcaca aaaacattac attccggcaa gttatgacag aattactgaa aacagtcatc
      301 tgtagtaatt gtcgtttctg atatatgctc atccatccgg aaatacgaca tggtcctgac
      361 tggcgttcac ttcaaagtgt aatttattat tcacacactg agactgtaaa acggatggcg
      421 tggatgagta cacagtctga agagtataaa aggaacagct ccggattgtt acccgggatt
      481 actatagttc accacacaac cgtaaacctg gttgtgtggt gaaacagtaa catattaaga
      541 atttgtgtta ccacctgttt tctcaggaaa cggattgcag gagaggtata ctcattcatc
      601 ttcccggagt tttgttaatc actgcataat caaacggacc aatacattct ttgctcgcat
      661 ccgtaaaaat gtcgccacaa tatcatcagt cagtcaactc attaccatct gttttccggg
      721 tccgtagtta ctacagtgct ccggagtgat aaacagaaac tgataatgaa gcataagaat
      781 acttttatta tgcgctgact gacatacttt acttaattgc gattctgccc tcaataaatc
      841 cggactgatt aatcatcatc tgatgaggtg ttatgccaaa atattctctg aaaacgctga
      901 taaaatacgg tgtgctggca taaccgcaac gtattgcgat ttgattcact gacagtttac
      961 tggaaataag ttgtcgggcg ttatgcatgc gtatatccag caggatttgg gagaaagaaa
     1021 ctttttcctc ctgaagtttt cttttcagta aactctcact catgtacaga gaattactga
     1081 tatctttaac tctccatacc cgtgatggac tggaacagat aatatacctg actttaagcg
     1141 caacagagtt tatgcatctg aatagcagaa acatgaaacc tctgtgtgtg gaaaaaatgg
     1201 aaaggcaggc aaaaaacatc tgctcagaaa attgttgtgg caatgtcttt tgcagactga
     1261 gattctgtat gacattatgt aacagagcga tgtgagagaa ggttgtttta atgaacggaa
     1321 cagaaacagc actatcagag tgctggatcc tgtcggcact ttccatatat tttctgacaa
     1381 tgcattcgtc aatccggagc tcagtaacat taccgggatg gcgcaggaaa aaggactggc
     1441 tgatgttatc tgtcaacagg acgtcatttt tctgaaaaaa tatctttttc ccggcaatct
     1501 ctgtttcaaa cggacggtta acaagaataa cgtaacagac aatcatcaca catactccct
     1561 gattagcata aaaacaacga tatcaaaaga ctagacgctt tttcaggaaa aatcttattt
     1621 gtccggagat tattcttacg gggccgggcg aatgctatct gaatcgtacg gttcatagaa
     1681 ataaatattt accgacatag ccggagggtt ttcagatgca atccctgact cactgcgaaa
     1741 tcgcatcgta tccgatcgcg gactcgccga ttctcagaac aggcagtccc tgagaatcgc
     1801 agaacgctag tgcacgatcg ttgaacacat cagctacggt gatcgatgtt ttactcgtat
     1861 tatagtttgc agtgtgccac tttcggggat gtagctacga acgtctgctg gcttcccgat
     1921 atgtcagtaa aaa
//
LOCUS       DAZFKF010000285         1921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFKF010000285 DAZFKF010000000
VERSION     DAZFKF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.288"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..1921
                     /locus_tag="ACHKA1_005466"
                     /pseudo
     CDS             1..1921
                     /locus_tag="ACHKA1_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          415 a          614 c          599 g          293 t
ORIGIN      
        1 ctggctggtg cgcggcggcg tggcgaaact ggatgaaggt caccggctgg ccgcactctg
       61 gcaggcgctg ccggaagagc ttcgcctgag tccgcatcgt tatctggcga caaacagtcc
      121 gcaggggccg tggtggctgc tcggctggtg tgagcgggtg ccggaagcgg atgaggtgct
      181 gcctgcgccg ctgccgccgt accgggtact gaccgggctg gtggaccgct tcgggcgcac
      241 acagacgttc caccgcgaag ccgccggtga attcagcggc gaaatcaccg gcgtgacgga
      301 tggtgccggg cgtcacttcc ggctggtact gaccacgcag gcgcagcggg cagaagaagc
      361 ccggcagcag gcctcttccg gcgggacgga accgtccgct tttcctgata ccctgccggg
      421 ttacaccgaa tatggccggg acaacggcat ccgtctgtct gccgtgtggc tgacgcacga
      481 cccggaatac ccggagaatt tacctgccgc gccgctggtg cgctatggct ggacgccacg
      541 cggcgaactg gcggcggtgt atgaccgcag caatacacag gtgcgcagct ttacttacga
      601 tgataaatac cggggccgga tggtggcgca ccgtcacacg ggccgaccgg aaatccgtta
      661 ccgttacgac agcgacgggc gggtgacaga gcagcttaac ccggcgggct taagctacac
      721 ctactgttat gagcaaaacc gcatcaccat caccgacagc ctggaccgcc gtgaagtgct
      781 gcacacgcag ggcgaaggcg ggctgaagcg ggacacagta cgatgcgtgg ggaaaggcca
      841 tccgtaccac gcagggcggg ctgacgcgca gtatggaata cgatgctgcc ggacgggtca
      901 tccgcctgac cagtgaaaac ggcagccata ccaccttccg ttacgatgta ctcgaccggc
      961 tgatacagga aaccggcttt gacggccgca cacagcgtta tcaccacgac ctgaccggca
     1021 aacttatccg cagcgaggat gaggggctgg tcacccactg gcactatgac gaagcagacc
     1081 gcctcacgca ccgcaccgtg aatggcgaaa ccgcagagcg gtggcagtat gacgaacgcg
     1141 gctggctgac agacatcagc catatcagcg aagggcaccg ggtgacggtg cattacgggt
     1201 atgatgagaa aggccggctg accggtgagc gtcagacggt gcatcacccg cagacggaag
     1261 cactgctctg gcagcatgag accagacacg cttacaacgc gcaggggctg gcgaaccgct
     1321 gtataccgga cagcctgccc gccgtggaat ggctgaccta cggcagcggc tggctgtcag
     1381 gcatgaagct cggcgacaca ccgctggtgg atttcacccg cgaccgcctg caccgggaaa
     1441 cgctgcgcag cttcggccat tatgaactca ccaccgctta tacccctgcc gggcagttac
     1501 agagccagca cctgaacagc ctgcagtatg accgcgatta cacctggaac gacaacggcg
     1561 aactcatccg catcagcagc ccgcgccata cccggagtta cagctacagc aactccggca
     1621 ggctgaccgg cgttcacacc accacctcaa ctctggatat ccgcatcccg tatgccacgg
     1681 acccggcagg taaccgcctg cccgacccgg agctgcaccc ggacagcacc ctcagcatgt
     1741 ggccggataa ccgtatcgcc cgtgacgcgc actatcttta ccggtatgac cgtcacggca
     1801 gactgacaga gaaaaccgac ctcatcccgg aaggggttat ccgcacggat gatgagcgca
     1861 cccaccggta ccattacgac agtcagcacc ggctggtgca ctacacgcgg acacaatatg
     1921 c
//
LOCUS       DAZFKF010000286         1920 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFKF010000286 DAZFKF010000000
VERSION     DAZFKF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.289"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            103..345
                     /locus_tag="ACHKA1_005467"
     CDS             103..345
                     /locus_tag="ACHKA1_005467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682966.1"
                     /translation="MMMARDITAYHLNSVHISARRFPEIFQWATGMQTKNLPLTNSLL
                     QNDYAESIFVFNGERDVSEMPLPEEMLLTGNAESER"
     gene            complement(1271..>1920)
                     /locus_tag="ACHKA1_005468"
     CDS             complement(1271..>1920)
                     /locus_tag="ACHKA1_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO5682967.1"
                     /translation="SANTYADQKETAINNRTDGLLMTERQARTAGDAATLAVARTHSD
                     ENNKRTLISANTYADQKETAINNRTDGLLTTERQARIAGDAATLATAATYSDAGDSLT
                     LKQATAYSDISTRRALSEANRYTDNKFRNLSNKIERAEKRLNAGIAGVTAISSIPYVA
                     ENSFSYGIGVGNYQNANAVAAGIQYKTSRNTNIRLNISWDSSHNTAIGAGVAGGW"
BASE COUNT          441 a          401 c          482 g          596 t
ORIGIN      
        1 agcagtaact ggctatcgtg tataattatc cagaagtcct gctgagcctt ccgtctgttc
       61 atcatgtgcc aggtttgcgt tcgggatatg atgtgccatc ttatgatgat ggcaagagat
      121 attactgcgt accacctcaa ttctgtacac atcagtgccc gtcgtttccc cgagattttt
      181 cagtgggcca ccggtatgca aacaaaaaat ctgccgttaa ctaattcttt gttacagaat
      241 gattacgcag aaagtatttt tgtatttaat ggcgaacgtg acgtttcgga aatgccttta
      301 ccggaagaaa tgctactgac tggaaacgct gagtcagaaa gataattcgg tgtattcatg
      361 actgttatca atgtgttatg tgtatgatgt caacgacgga tgaaaagtta tccacttata
      421 tgaggcatta taggattatt tctggctgac gttgtttcag ttcagaccag gggcagtgaa
      481 tatgctgacc tgctttttcg ctgatatcag tccccctgta attacataga taatagatat
      541 acctctccgt tgatcaggct gtccaggtaa ttgataccgc acttcctcat cagttcgtag
      601 cagagtgtac ttagcatggc tagtgcagtt tctgatgatt tacagtgtgt attcaggaga
      661 gtaagaaatt gctcaaaaca tcctggttta tactggtgct ttgatattca ttgtgtccct
      721 tttccgttcg ttattatttc catatttaat atgatgcctg tacatgtaaa ataatggaag
      781 atttcagggg agcattgcat aaagtccgcc tggagaataa ggctcaggtc agcgaatcgc
      841 atcgtggcca gactgcactg actgcaataa gggcggatac agagttgcag ctacttgtcg
      901 atttcagaac aacagaatcc ttgcaaacag ggatgtgttc attcaggggt tatgatgcta
      961 cgacgaacgc ttttagtgat tattaatcga cgatactgtg ttttgtctgg atggtgaggg
     1021 actcgctttg aggattcacc agggaaatac ggggcttgtg gcaataatgt tagcctgcga
     1081 acttaaatgt ttacgggcaa catggcggca ggaaatctgc cgccggattg ttgtgaaggc
     1141 tgaccacact gcgcgccctt tttccttgat tatcctgctc agtccagtgg tctgttccgg
     1201 atggggctgt gttgcgtact gaattaatgt gattatggaa taccataatg ccgtatgtac
     1261 gcaagattat ttaccagccg ccggcaaccc ctgcaccaat cgcggtgtta tgagatgagt
     1321 cccaggagat gttgaggcgt atattggtat tacgtgatgt tttatactga atgcctgcgg
     1381 ccacagcatt agcgttctga tagttaccca cgccgatccc gtatgaaaaa gaattttcag
     1441 ccacataggg gatggaggat attgctgtta ctccggcgat gcctgcatta agtctttttt
     1501 ctgcccgttc aattttattg ctcaggtttc tgaatttgtt gtcggtataa cgattggcct
     1561 ctgacagtgc ccggcgggta ctgatatcgc tgtaggctgt tgcctgtttc agtgtcagtg
     1621 aatccccggc gtcactgtat gtcgctgctg ttgccagagt tgcggcatca cctgcgatgc
     1681 gtgcctggcg ttcggtcgtc agtaatccgt cagtacggtt attgatggcg gtttctttct
     1741 ggtcagcgta ggtattcgca ctgattagtg ttcttttgtt gttttcatcg ctgtgcgttc
     1801 tggcaactgc cagcgtcgca gcatcacccg cggtgcgtgc ctggcgttcg gtcatcagta
     1861 atccgtcagt acggttattg atggcggttt ctttctggtc agcataggta ttcgcactga
//
LOCUS       DAZFKF010000287         1905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFKF010000287 DAZFKF010000000
VERSION     DAZFKF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.290"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            328..1863
                     /locus_tag="ACHKA1_005469"
     CDS             328..1863
                     /locus_tag="ACHKA1_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5682968.1"
                     /translation="MSCIKEKSKYAFERAKQFYFMLWCKESLIYLLFSVLVTWCCGYT
                     VKNALGNVLILFLMMLLGRRNVISATCVCIFLLLSAFYAPVGMIYGKINNSFIASVMQ
                     TNINEAGEFITGIPVLYFIVSLVIAAFTFWFWRKRDKSHRGFIFLLFFIVLSVNAWPK
                     RMVTNVMTGIAETREEMMRYESLKNNQKDSWDIVSVEKKYKTIIIVIGESVRRDYLSV
                     YGYPLPTTPWLNSAPGIFINGYFSAAPNTIGSLSRTLTLDYTETGNPGNNIVTLARKA
                     GYETWWISNQGSLGQHDTLISVIAANADKKHFLKEGSSSSLRIDDEDMLQYVREAVNS
                     SSPKVIFIHMLGSHTNPCDRLFNDAQPFRELFGHKVSCYLSTLLKLDRFLQAIYNMTT
                     EHGDDFAMLYFSDHGQSVSSDSTPVHHAPGLRSGYDVPLMMMASDITEHHLNHVHISA
                     RRFPEIFQWITGIQTKNLPLSFSLLQNDFAESVSVFNGERDVPVMSLPEEPILTGNAE
                     SEK"
BASE COUNT          513 a          330 c          445 g          617 t
ORIGIN      
        1 atgttgtcgg tgctgagtgt atggcaaccg gaaagattga gcatattttg attgatatta
       61 ttatagccat gctttctctt tcgtatgttt gcctttccgg gagggcaaga catatttttg
      121 ttaactaaat gtattctgag tgtttgatgg ttatgaggcc ttcatcgggt tatgcgaata
      181 atctggttcg gggagcagtt gctgtaaaca cagaacctga taatatgtaa accctgctgg
      241 acagggtagg gtatttttgt gaactgtttc atataaatat ccatgcgttg aaatgatcgt
      301 ctgtgtttgt acaggaaaat gactggcgtg tcttgtatta aagaaaagtc gaaatatgct
      361 tttgagagag caaaacagtt ttattttatg ttgtggtgta aagagtcact gatttatctg
      421 ttgttttctg tacttgttac atggtgctgc ggatatacag tgaaaaacgc cctggggaat
      481 gtgcttatat tgttcctgat gatgttgttg gggcggcgga atgttatttc agcaacgtgt
      541 gtctgtatct ttcttttgtt aagtgcattc tatgctccgg ttgggatgat ttacgggaaa
      601 ataaacaaca gttttattgc gtctgtaatg cagacaaata taaatgaggc aggtgagttt
      661 atcacaggca tacctgttct ttattttatt gtttcgctgg tcattgctgc ttttacattc
      721 tggttctggc gtaagcgtga taaaagccac cggggattta tctttctgtt gttctttatc
      781 gtattgtcag taaatgcatg gccaaaacgt atggttacga atgtaatgac agggatagct
      841 gaaaccagag aagaaatgat gcgatatgaa tcgctgaaaa acaatcagaa agactcatgg
      901 gatattgtga gtgtggagaa gaaatataaa accataatta tcgtaattgg tgaaagtgtc
      961 agacgggatt atctttctgt ttatgggtat cctttgccga caacaccatg gctgaattct
     1021 gctccgggta tatttattaa tgggtatttc tcagcagcgc cgaatacgat aggttcactc
     1081 agcagaacac tgacgcttga ttacactgaa acaggtaatc cgggtaataa tattgttacg
     1141 cttgccagaa aagccgggta tgagacgtgg tggatatcaa atcagggctc tttagggcaa
     1201 catgatacgc tgatttctgt catcgccgcc aatgcggata aaaaacattt tcttaaggaa
     1261 gggagctctt cttcccttcg cattgatgat gaggatatgc ttcagtatgt cagggaagct
     1321 gttaactcgt cttctccaaa agtgattttt attcatatgc tggggtctca cactaatccc
     1381 tgtgaccgac tttttaatga tgcacagcca ttcagggaat tattcggaca taaagtatcc
     1441 tgttatttat caacactgct taaactggat cggtttcttc aggcaattta taacatgacg
     1501 acagagcatg gtgatgattt tgccatgctg tatttttctg atcatgggca atccgtcagt
     1561 tcagacagca cccctgtgca tcatgcgcca ggtttgcgtt cgggatacga tgtacctctc
     1621 atgatgatgg ccagtgatat tactgagcac cacctcaatc atgtgcatat cagtgcacgt
     1681 cgttttcccg agatttttca atggatcaca gggatacaaa ccaaaaatct gccgttaagt
     1741 ttttctttgt tacagaatga tttcgctgaa agtgtttctg tattcaatgg tgaacgtgac
     1801 gttccggtaa tgtctttgcc agaagagccg atactgaccg gaaatgctga atcagaaaaa
     1861 taataaagat tattgatgac cgtgctcaat gcctcatgtg tatga
//
LOCUS       DAZFKF010000288         1868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFKF010000288 DAZFKF010000000
VERSION     DAZFKF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.291"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            119..373
                     /gene="bssS"
                     /locus_tag="ACHKA1_005470"
     CDS             119..373
                     /gene="bssS"
                     /locus_tag="ACHKA1_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO5682969.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            488..1606
                     /gene="solA"
                     /locus_tag="ACHKA1_005471"
     CDS             488..1606
                     /gene="solA"
                     /locus_tag="ACHKA1_005471"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO5682970.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1654..1767
                     /locus_tag="ACHKA1_005472"
     CDS             1654..1767
                     /locus_tag="ACHKA1_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO5682971.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          483 a          454 c          442 g          489 t
ORIGIN      
        1 tttccccctt taaaaattcg gtgaataccc ttacttattg gtatactgaa gttgcttcag
       61 ataaaaacgc attgaacctc gaataacgtt gtctagtaac acgaattagg gggccatgat
      121 ggaaaaaaat aatgaagtca ttcagactca tccgctcgta gggtgggaca tcagcaccgt
      181 tgatagctat gatgcgctga tgttgcgttt gcactaccag accccaaata agtccgagca
      241 ggaagggact gaagttggtc agacgctctg gttaaccact gatgttgcca gacaatttat
      301 ttcgatatta gaagcaggaa tcgccaaaat tgaatccggt gatttcccgg taaacgagta
      361 tcggcgtcat taacatgaca tgaccatcca cccaaaaggc acctcaccgg tgcctttacc
      421 atttctgggt tgtatatctg cattcagtta attactctgc ttaaaccatg agagcagaga
      481 gaaaaaaatg aaatacgatc tcatcattat tggcagcggt tccgtaggcg ctgccgccgg
      541 gtattatgca acccgcgccg gtttaaacgt gcttatgacc gacgcccata tgccaccaca
      601 tcaacacggc agccaccacg gcgatacgcg attaattcgc catgcttatg gtgaaggcga
      661 aaagtatgtc ccactggtcc tccgcgcgca aacactgtgg gatgagctct cccgccacaa
      721 cgaagatgat cccatttttg tacgctctgg tgtcattaac ctcggcccgg ctgactccgc
      781 atttctcgcc aacgtcgccc acagcgccga acagtggcaa ctcaacgttg aaaagctcga
      841 tgcgcaaggg attatggccc gctggccaga aatacgcgtc ccggacaatt acatcggctt
      901 atttgagact gattccggtt ttttgcgcag cgaactggcg attaaaacct ggatccaact
      961 ggcgaaggaa gcgggctgtg cgcaactgtt caactgcccg gtcaccgcaa ttcgtcatga
     1021 cgatgatggc gtaactattg aaacggctga cggtgagtat caggcgaaaa aagcgattgt
     1081 ctgcgcggga acatgggtaa aagacctgct cccggagctg cctgtccagc ccgtacgtaa
     1141 agtatttgcc tggtatcagg ccgatggccg ctatagcgtg aagaataaat tcccggcgtt
     1201 taccggtgaa ctgcccaatg gcgatcaata ttatggtttt ccggcagaaa acgacgcgtt
     1261 gaagattggc aaacataacg gaggccaggt tatccattca gcggatgaac gtgttccgtt
     1321 tgcggaagtg gccagtgatg ggtcagaagc cttcccgttc ttgcgcaatg tattgccggg
     1381 tatcggttgc tgcctgtacg gcgctgcctg cacctatgat aattcgcctg acgaagattt
     1441 tattatcgat accctacccg gccacgataa tacactgctc attaccggcc tgagtgggca
     1501 cggttttaaa tttgcgtcag ttttagggga aatagctgcc gattttgcgc aagacaaaaa
     1561 aagcgatttt gatttgacgc cattcaggct ttcccgcttc caataatcat cattacggcc
     1621 tccagaatga ggccgttttt acaggagtat ttaatgcgtc ggctgttgca ctatctcatc
     1681 aataatattc gcgagcatct gatgctttat ctttttctct ggggattgtt ggcaattatg
     1741 gatctgatct atgtgtttta tttttagttt tccgcactga cataaactga caaatattta
     1801 atctcccttt catttagcgt gaaaaattga atgagattat tcctgcttct acagcactca
     1861 ccagacat
//
LOCUS       DAZFKF010000289         1838 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFKF010000289 DAZFKF010000000
VERSION     DAZFKF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.292"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            366..803
                     /locus_tag="ACHKA1_005473"
     CDS             366..803
                     /locus_tag="ACHKA1_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUKH-3 domain-containing protein"
                     /protein_id="HFO5682972.1"
                     /translation="MCELINEASKYGKVNVRKMDIDLCCKKIEEEGYSVFVAFYDFIK
                     KYGGLYGFHSAYKDTTISEAFFIDPERASNRIFIERVNDYIERAGYNMIPVGECSNGH
                     ITLLYGENNVIYGGLDDYLWKYGDNLSSSIDILINGKEAVEIP"
     gene            865..1086
                     /locus_tag="ACHKA1_005474"
     CDS             865..1086
                     /locus_tag="ACHKA1_005474"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HFO5682973.1"
                     /translation="MSDTSSNRITFERDSQYRITGVSHTDGIRLKLTYHASGYLKAIH
                     RTGNGIQTQGKIIRSTLFVLNNSHFMFKN"
     gene            1165..>1838
                     /locus_tag="ACHKA1_005475"
     CDS             1165..>1838
                     /locus_tag="ACHKA1_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO5682974.1"
                     /translation="MELKKLMEHISVIPDYRQPWKVEHKLSDILLWTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVYCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPGLLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYA"
BASE COUNT          584 a          319 c          411 g          524 t
ORIGIN      
        1 attcttagaa tggattgtaa aagatggtta tttagaggaa ataattttgt ctggttcata
       61 aattcatctt ctttgttgag aagattaact gcctaggtga aattatcctc tatgaccggg
      121 acgctgcagg ccacctggca gaagtcatca cgcaggaaga caagtcgctg tatccctttg
      181 aaaaatccgg caggctaatg ggcatcttca gcccaggcgg cacatcacag cgaatttgaa
      241 ggcaaatgcg aaaattagtg atcttgaagt tcatacagta ttggtaaaaa caggcgaagc
      301 ttttccaatg tgtaagaatt ttcaaatttc aatatttggc gctgttgtta caagcgggtg
      361 attatatgtg tgaattaatt aatgaagcat caaaatatgg gaaagtcaat gtaagaaaga
      421 tggacattga tttgtgttgt aaaaaaatag aggaagaagg gtattctgtt ttcgttgcgt
      481 tctatgattt tataaaaaaa tatggcggtt tatatggctt tcattcagct tacaaagaca
      541 ccactataag tgaggctttt tttatagatc cagaaagggc ctctaatcgc atttttatag
      601 aaagagtcaa tgattatatt gagagagctg gttacaatat gattcctgtt ggcgagtgca
      661 gtaatggtca tatcacatta ttatatgggg aaaataatgt gatatatggc ggcttggatg
      721 attatctttg gaagtatggg gataatttat cttcatcaat tgatatattg attaatggca
      781 aggaggcagt tgaaattccg taaggccaac ttgatatgac acctggcaca caaccccgac
      841 gaccggatgt atatgctggg cgggatgagt gacacctcca gcaaccgcat cacgtttgag
      901 cgcgacagcc agtaccggat cacaggtgtc agtcacaccg acgggatccg gcttaaactg
      961 acgtaccacg ccagcggcta cctgaaagcc attcaccgca cgggtaacgg catacagacg
     1021 cagggaaaga tcatcagaag tacacttttt gtactaaata attcgcattt tatgtttaaa
     1081 aattgagata ttcctcatta cctgaagctg ttttttattg cttatacatg atcaaatact
     1141 ccttacataa ttaaggagaa caaaatggaa cttaaaaaat tgatggaaca tatttctgtt
     1201 attcccgatt acagacaacc ctggaaagtg gaacataaat tatcggatat tctactgtgg
     1261 actatttgtg ccgttatttc tggtgcagaa ggttgggaag atatagagga ttttggggaa
     1321 acacatctcg attttttgaa gcaatatggt gattttgaaa atggcattcc tgttcacgat
     1381 accattgcca gagttgtata ctgtatcagt cccgcaaaat ttcacgagtg ctttattaac
     1441 tggatgcgtg actgccattc ttcagatgat aaagacgtca tcgcaattga tggaaaaaca
     1501 ctccggcatt cttatgacaa gagtcgccgc aagggagcga ttcatgtcat tagtgcgttc
     1561 tcaacaatgc acagtctggt catcggacag atcaggacgg atgagaaatc caatgagatt
     1621 acagctatcc ctggacttct taacatgctg gatattaaag gaaaaattat cacaactgat
     1681 gctatgggtt gccagaaaga tatcgcagag aagatacaaa aacagggcgg tgattattta
     1741 ttcgctgtaa aaggaaatca ggggcggctt aataaagcct ttgaggaaaa atttccactg
     1801 aaagaattaa ataatccaga gcatgacagt tacgcaat
//
LOCUS       DAZFKF010000290         1836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFKF010000290 DAZFKF010000000
VERSION     DAZFKF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.293"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1110
                     /locus_tag="ACHKA1_005476"
     CDS             <1..1110
                     /locus_tag="ACHKA1_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5682975.1"
                     /translation="GDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELARTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVKQMQNQ
                     MDPVYTPARKIHLYHCDHRGLPLALISTEGTTAWYAEYDEWGNQLNEENPHQLQQLIR
                     LPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLA
                     SKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSP
                     YDYTLYCRKWVKPNLTCTPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYL
                     FPDINKPDGPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            1107..1676
                     /gene="ybfC"
                     /locus_tag="ACHKA1_005477"
     CDS             1107..1676
                     /gene="ybfC"
                     /locus_tag="ACHKA1_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO5682976.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWKWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKDESYECLTKIQHELLRSEEK"
BASE COUNT          567 a          398 c          445 g          426 t
ORIGIN      
        1 ggcgaccgcc tgaccacgat acagaacgac agaacccgca tccagacgat ttatcagccg
       61 gggagcttca caccactcat cagagttgaa actgccaccg gtgaactggc cagaacgcag
      121 cgccgcagcc tggcggatgc ccttcagcag tccggcggtg aagacggtgg cagtgtggtg
      181 ttcccgccgg tgctggtgca gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg
      241 gtgagtgagg aaagccgccg ctggctggca tcgtgcggcc tgacggtgaa gcagatgcaa
      301 aaccagatgg acccggtgta cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat
      361 cgcggcctgc cgctggcgct tatcagcacg gaagggacaa cagcgtggta cgcagaatac
      421 gatgaatggg gtaaccagct gaatgaagag aacccgcacc agctgcagca gctcatccgc
      481 ctgccgggtc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca ccgctattat
      541 gacccgctgc aggggaggta tatcactcag gatccgattg gactgaaggg gggatggaat
      601 ttttatcagt atccgttgaa tccggttcag tatatagatt caatgggact ggcatcaaaa
      661 tatggacact taaataatgg cggatatgga gcgagaccca acaaaccgcc tacgcccgat
      721 ccaagtaaat tgccagacat agcgaaacaa ttaagactgc catatcctat tgaccaggcc
      781 agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta cgactacaca
      841 ctgtattgca ggaagtgggt aaaaccaaat ctgacttgta cgccacagga tgatccccag
      901 tatccaggga tggatacaaa gacagcaagt gattacctgc cacagacaaa ttggccaaca
      961 actcaattac caccaggata tacttgtgca gaaccctatt tattcccaga cattaataaa
     1021 cccgatgggc cagcaacagc agggatagat gatttgggtg aaattttagc taaaatgaaa
     1081 cagagaacat cgagaggaat aagaaaatga aaagagtttt gttctttttg ctgatgatat
     1141 ttgttagttt tggtgttata gctgattgcg aaatacaagc taaagatcat gattgtttta
     1201 ctattttcgc taagggtact attttttccg cgtttcctgt cttaaataat aaagccatgt
     1261 ggaaatggta tcagaatgag gacatcggtg agtattattg gcaaacagaa ctgggtacat
     1321 gtaaaaataa taaattcaca ccaagcgggg cgagactctt aattcgcgtc gggtcactac
     1381 gccttaatga aaatcatgcc atcaaaggaa cgttgcagga gttgataaat acagcagaaa
     1441 aaacggcgtt tctcggcgat cggtttagaa gttatataag agcgggtatc tatcaaaaaa
     1501 agagcagtga tccagctcag ttgttggcag tccttgataa ttcaatcatg gttaaatatt
     1561 ttaaagatga gaagccaact tatgctcgaa tgacagcaca tctcccgaac aaagatgagt
     1621 cttacgaatg tttaacaaaa atacagcatg aactgcttcg tagtgaagaa aaataaaaca
     1681 tcttcctgag agaataaatt aaccattcgg aaaaacatgt tcttaatgga tatccaggta
     1741 caccagaatg tcgatttgga aaaatggatt ccgatggtaa gaatatcata tattctgaat
     1801 gtaatgtttt acccacagct tttgagattc gatagg
//
LOCUS       DAZFKF010000291         1826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFKF010000291 DAZFKF010000000
VERSION     DAZFKF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.294"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            41..673
                     /gene="yqeH"
                     /locus_tag="ACHKA1_005478"
     CDS             41..673
                     /gene="yqeH"
                     /locus_tag="ACHKA1_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5682977.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            1007..1816
                     /gene="yqeI"
                     /locus_tag="ACHKA1_005479"
     CDS             1007..1816
                     /gene="yqeI"
                     /locus_tag="ACHKA1_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5682978.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAGCLTLSYREVNRD"
BASE COUNT          602 a          328 c          348 g          548 t
ORIGIN      
        1 tgaaattatg caattatata cggataggga ggttcttaac atggggcttt gtagtcgtta
       61 taaaagtctt acatgcaata gttgctcaat gcattgccaa ataatgccag aagagtcacc
      121 gcgtttgcag tattgcgcaa actcgtgttt ttgtatgtgg cccgaagaaa gttcatattt
      181 taatcgtggt gtggtggaag gtatcttaac aaaaaaccac aatgcgagat taagcggata
      241 tatttttgtc gatttttcag taagtttttt acgtctattc cttgaaaaag actggattga
      301 ctatcttgca agtactgaca tggggattgt tttagtcagc gaccgcaata tgcaatcgtt
      361 agctaactac tggcgaaagc acaactctgc aatatctgct gtcatatata atgatgatgg
      421 tcttgatgtt gccaatgaaa agatcagaca actatttatt ggtcgttatt tatcatttac
      481 cagaggtaac acattaactc agatggaatt caccataatg gggtatatgg tttctggtta
      541 taacccatat cagattgctg aagttctgga tatggatatc cgtagcatct acgcgtacaa
      601 gcaacgaatc gaaaagagaa tgggtggtaa aataaacgag ttatttattc gttcacattc
      661 ggtccaacat taatactcaa cgatctgcca gaatcatact ctgcttatac gggcatctat
      721 tggaatgccc gtattttata aataagcagt aaccccaaga gtaaacaaca tagtatttaa
      781 aaccatcaca cgaaaaattc agaagcatta cgaaatatga atttatcaac aatagtggca
      841 taaatgttaa ccatgttaat ttatataaag ttttacgttg cgccattaaa gcctcatttc
      901 aatcatcatg ataaatataa aattaatata tatttatgcc gtaaatacca atatacttag
      961 caaactatgt gatttccatt tcgattgatt tagtgtttat tgacgtatgt actggattat
     1021 taacgataat atcgagttct ggcctgagca ccgaaaatta atatcggtac ataacgccga
     1081 tcttaacgtc gttctgacaa cgccagccag tcgttgttta tcccttctac ttgaagcttt
     1141 tcctgatgtg gttgcacaac aagatttttt caccagagtc tgggaagaag aaggtatgcg
     1201 tgtgcctact aacacgttat atcagaacat atccattatc agacgcggat ttcgcgctgt
     1261 tggtgatact acccactcgc ttattgcaac cgtgccgaga agaggattca agatccataa
     1321 tgacattaac atacaaaatc atgtaataaa ctcgtcaaca gacgcacata cacacaatgc
     1381 cccaccggcc ataaaagtta atgcgggata caaagagagc attggtggcg caaagaattt
     1441 caataacaaa atcctcaaac atataaaaag ttatctaatt atgttgagcg catttgtcat
     1501 aggcgcatat tctgcatatt ggctatggaa taataatcaa ccgaagccat ttttcaagga
     1561 ttacaagact gttgcggaaa ttaatggctg ccattttaat gtaacagaag atacaattga
     1621 tgggttgaaa gagttcgata aatataaaac acggatactg gattcgggga ttaactgtaa
     1681 aaaatatccg tggttatact tccctcttgc aaaatcctca ccggggatga ttgttatggc
     1741 atgcaataaa aactataacc aacatgaagt ggcaggctgt ttaaccctgt cttaccgtga
     1801 ggttaatcgt gattgattat aaaaaa
//
LOCUS       DAZFKF010000292         1790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFKF010000292 DAZFKF010000000
VERSION     DAZFKF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.295"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(168..1355)
                     /locus_tag="ACHKA1_005480"
     CDS             complement(168..1355)
                     /locus_tag="ACHKA1_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFO5682979.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLADMSRIAADVIQEICRQADVVPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTPDHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVIPDALAVEGNSGR
                     DWNRFLDSHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEDVVYVITE
                     TVRKTAMQIRWRGMYQRLLKVDPLKCILCGCQMRFTGLKRGYRLTELVLMHEPLAQQR
                     VCG"
     gene            complement(1355..1651)
                     /locus_tag="ACHKA1_005481"
     CDS             complement(1355..1651)
                     /locus_tag="ACHKA1_005481"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682980.1"
                     /translation="MKLHDDDFFDEEDAEALRFDNFDDAVECCADLNIPFFVDAGNKK
                     LVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMYCPECGTVHWPDGVPPPF"
BASE COUNT          430 a          529 c          422 g          409 t
ORIGIN      
        1 caaaagatta aaaaaacacc aaaaaaacgg atgtttattc aacactgcct gcgtgccatg
       61 agtgcaccgc catcaattaa atggatctta tcgattgata ctgtatatca ctgatggttt
      121 tttactccat tcccctgaaa atggacgcaa cttccccgat gcggctctca gccgcacacc
      181 cgctgttgcg ccagtggctc atgcatcagg accagctctg tcagacggta gccccgcttc
      241 agccccgtaa aacgcatctg acatccgcac aggatgcact tcagcgggtc aaccttcagt
      301 aaccgctgat acatccctct ccacctgatt tgcatcgccg tctttctcac cgtctccgtt
      361 atgacgtaca caacatcttc cagtaaccgg cgcttcaccg gactcaggaa accgtagtac
      421 ctcaccatac ggaacccctt atccgccaca tgccaggaga acctttccat aaactcatca
      481 ccactcatca ccaggtattc ttcccgtttt gtccggtgac tgttgtaacg cagaccaatt
      541 tcatcctgac cagcatagtg ctccagacgg ctcatcggca ccggcggttt tttcaggtaa
      601 gagccgaagt acaccgccac atgtgtggcg ttatccatca cccgggatac gttgacattc
      661 cagccccgcc ggtaatgact gtccaggaag cgattccagt cccgtccgct gtttccttca
      721 actgccagcg catccggtat caccaggtca gggtatttcc gtgacagcaa ccgtgttatc
      781 cggtaacgcc acatgctcat caccttacgg gcgtaaaaat gaaggttttt ccaggtgtgg
      841 tctggtgtca cgccgccggc cgttgtcgac aggtgaatgt gcggatgcca ctgctggtca
      901 cgtccccatg tgtggatcac cgtgaatatc cccggcacca catctgcctg gcggcagatt
      961 tcctgtatca catccgcagc aatgcggctc atatctgcca gtaaccaccg gttgtggaac
     1021 accagggacc agtactggca gggaagtgtg aacacaatat gctgccacgg acagtcggga
     1081 accagactca gcagatactg tatccactgt gcgccagcct tcactccgca gtgcgggcag
     1141 gagcgacttt tacaccggaa gcagaccttt tttgtgtggc aacagtccgg agatgaacag
     1201 caccactgtg tgtaccccat cagcgtggtt ccgcacgcca tgatttttgt caccgactca
     1261 atgaccaccg gacgcactga accttccggt tgtttctcca gccagttaag ccagcggttt
     1321 ccctgctgaa aaatgtcggc aaaacgggga agcatcagaa gggcgggggg actccgtccg
     1381 gccagtgaac tgtgccacac tccgggcagt acatgccgcc ggcgctgata ccggcaagaa
     1441 tggtcgcaaa ctcccgctcc gtgcagcggg ctatttcagg atacccttcg tcatcaacac
     1501 gaacaaacca gaagaccagc tttttgtttc cggcatccac aaagaaggga atattcaggt
     1561 ctgcgcagca ctcaacggca tcgtcaaaat tatcaaagcg cagagcttct gcatcttctt
     1621 cgtcaaagaa gtcatcgtcg tgaagcttca cgacatagcg gggaagtttg cttctttgag
     1681 gggcaggttt tcgtttacgg ggtttagctg aacgggccat ataaccaact gaaagacaat
     1741 gatattgtct gtttttataa cggtaagctc agaccatgac aagccgcagc
//
LOCUS       DAZFKF010000293         1719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFKF010000293 DAZFKF010000000
VERSION     DAZFKF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.296"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            665..>1719
                     /locus_tag="ACHKA1_005482"
     CDS             665..>1719
                     /locus_tag="ACHKA1_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase domain-containing protein"
                     /protein_id="HFO5682981.1"
                     /translation="MIPMKNYLFQTPEDICVQLKKMTHPVTIRTTDIANFWHYLESAT
                     LPVITKSTTTENREVTFLWRSEKALQGVYLRLNRVTDKKDVKKGLMTHIPSTDIWMLT
                     LVLPASYRGSYSFIEIPTDMTQKDIFQLGSRFSPLPGKSDPFNKTAEINIRGFGESVL
                     SLDMAPEQKEWDDTSHKCTGILSTLHSFVAGYQRRIRLYFPLNPTSVPLGLLVLPDAE
                     TWFDRIDITRALDMAITTGRITPMAIMGIDNINESDRMNILGGNKELIFDIAENLIPQ
                     LYRDYPDIVWAGRSNTVLAGQSLGGVTALMAAIYASTTFGTIISHSPSMWWNPDQGSP
                     ILFTENNTSWVSEQILSA"
BASE COUNT          555 a          339 c          334 g          491 t
ORIGIN      
        1 atgataaaca agaatatgtg tctgtcttaa gaggggatat tttaacaaaa ataaaactga
       61 atgatattct gaattctgtg ataataaatg gttgcaacac caaattaata tctgaacata
      121 aatgagcagg aggtctttaa aatgagagaa atatcagatc acatgcttga tttcgtgaaa
      181 ggagggatga atcttaatgg attacctagt tctactaatg taatagatct acgaggaaaa
      241 gatatgggaa catatattga tgctaatgga gcatgctggg ctccgggtac tccatccatc
      301 atcatgtatc cggggggaag tggaccttct tatagtatga gtagttccac atccagtgca
      361 aacagcggca gttaaataaa taccctccgg catggccgga gggtatttat gatgtgcctg
      421 taagactcaa cttctgtcgt ctctttacaa aaggcatatg atctgtcatt ttgtaccaga
      481 catcttaacg agagcctttt catatattac ttacatacgt gaaatctaca gtataaagca
      541 aaaattttat taaacctgtt ttatatttat catcaataca acagatttaa ttccggaaag
      601 tcactcatca tcccttgaat gaacgtcatt tgatgtatta ttcttacact tggattaatt
      661 taagatgata cctatgaaaa actatctttt ccagacaccc gaagatattt gtgtacagtt
      721 aaaaaaaatg acacatcctg tcacaataag aacaacagat attgctaatt tctggcacta
      781 tcttgagtca gcaacacttc cagtgatcac aaaaagcacc actacagaaa atcgggaggt
      841 tacatttctg tggcgctcag agaaagcact gcaaggcgta tatcttcgcc tgaatcgtgt
      901 aacagataaa aaagacgtca aaaaaggact aatgactcat atcccttcga cagatatctg
      961 gatgctgaca ctggtgttac cagcttcata tcggggctca tactcattta tagaaattcc
     1021 cacagatatg acacaaaaag acatatttca actaggaagt cgcttctctc cattacccgg
     1081 taaatctgat ccatttaaca aaacagcaga aataaatata cgaggattcg gagaatcagt
     1141 cctttctctt gatatggctc ctgaacaaaa ggaatgggat gatacttccc ataaatgtac
     1201 aggtattctt tcaacattac attcctttgt tgcaggatat caacgccgga ttcgtttata
     1261 ttttcccctg aatccaacat cagtacctct tggattactt gtgttacctg atgctgaaac
     1321 atggtttgac cggatagata ttacccgggc attagatatg gccatcacca ctggtcgtat
     1381 cacgccaatg gcaattatgg ggatagacaa tattaatgaa tctgatcgta tgaatatact
     1441 gggaggcaat aaagaactca tctttgatat agcggaaaat ctgatacccc agttatacag
     1501 agactatccg gatatcgtat gggctggtcg ctctaatact gtactggccg ggcagagcct
     1561 cggtggagtg acagcactga tggcagctat atatgcgtcg acaacatttg gtacaatcat
     1621 tagtcactca ccttcaatgt ggtggaaccc tgaccagggc agcccgatct tgtttactga
     1681 gaataatacc tcctgggtaa gtgagcagat actttcagc
//
LOCUS       DAZFKF010000294         1716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFKF010000294 DAZFKF010000000
VERSION     DAZFKF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.297"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            35..1456
                     /locus_tag="ACHKA1_005483"
                     /pseudo
     CDS             35..1456
                     /locus_tag="ACHKA1_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease fold toxin"
     gene            1458..>1716
                     /locus_tag="ACHKA1_005484"
     CDS             1458..>1716
                     /locus_tag="ACHKA1_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004014665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682982.1"
                     /translation="MSRYILIRGWFECDFEQVKKIKEANEEFFKHSEKYLLPENTKRL
                     YLNGWCYPEYPINWVSVISLGVNINQIAFDYIKDMIVNVSRT"
BASE COUNT          492 a          366 c          503 g          355 t
ORIGIN      
        1 agatatttct tggtaaggaa aatattcccc gaaagaggcc acatggggaa tactgctccc
       61 ctcactggta cgggagtacc gttacgacag cgcgggtaac gtatcaggcg tcaccagccg
      121 ggaagattac ggacgggaaa cacagcggga gtaccggctt gaccggaacg gccaggtcac
      181 ggcggtgaca gcctcaggca ccgggctggg ctatggcgaa ggcgatgagt cctatggcta
      241 tgacagttgt ggctacctga aggcgcagtc tgcgggcggg caccggataa gtgaagagac
      301 tgaccagtat gccggaggcc accggctgaa acaggccgga aacacgcagt atgactatga
      361 cgccgcaggc cggatggtca gccgcaccag acaccgtgac ggctatcgcc cggaaacaga
      421 gcggttccgg tgggacagcc gggaccagct gaccgggtat tgcagcgcac agggtgagca
      481 gtgggaatac cgccacgacg ccagcggcag acggacggaa aaacgctgtg accggaagaa
      541 aatccgtttt acgtacctgt gggacggcga cagtattgcg gaaatccggg aataccgcga
      601 tgatgaactg tacagcgtac ggcacctggt gtttaacggc tttgagctga taagccagca
      661 gtgcagccgg gtacggcagc cgcacccgtc cgtggccccg cagtgggtga cacgaacgaa
      721 tcatgcggta agcgacctga cgggccgccc gctgatgctc tttaacagtg aaggcaagac
      781 ggtctggcga ccgggacaga ccagcctgtg ggggctggca ctcagcctgc ccgcagacac
      841 agactacccg gacccgcgcg gggagctgga cgcggaagcc gaccccggcc tgctgtatgc
      901 aggacagtgg caggatgcag aatcggggtt gtgctataac cggttccgat attacgagcc
      961 ggaaaccggg atgtacctgg tgagtgatcc actggggttg ctggggggta agcagactta
     1021 ccggtatgtg ccgaatccac tggggtatat tgatccgctg gggctggtaa aaacttctgt
     1081 tcccgcagaa aaaataagcc tttcggataa agcaagagat ctttttagac aaggaaaagt
     1141 aagagaagca ttagatgttc attatgagga tcttgtacga agaaagcttg gtggtatatc
     1201 tcaagaaatt gccgggcgtg aatatgatgt tgttactgac aagatcatag cgcaggtcaa
     1261 gaggacttat agctctatag ataatccaaa aaacttctta agtaagtcta ctcggacaca
     1321 aattaagaag acaattgagc tggctgaaga acaggggaaa gaggctcagt tttggtttaa
     1381 atatggagta agccctaaag taagagaata tatagaaagc aaaggtggga aagttatatt
     1441 gggtatgggg aattaacatg tcaagatata tacttattag aggttggttt gagtgcgact
     1501 ttgaacaagt aaaaaaaata aaagaagcta atgaagaatt tttcaaacat tctgaaaaat
     1561 atttattacc agaaaacacg aaacgacttt atttaaatgg gtggtgttac ccagaatatc
     1621 ctatcaactg ggttagcgtt atttccttag gtgttaatat aaatcaaatt gcatttgatt
     1681 atataaaaga catgattgta aatgtttcaa gaactg
//
LOCUS       DAZFKF010000295         1686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFKF010000295 DAZFKF010000000
VERSION     DAZFKF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.298"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1077
                     /gene="cba"
                     /locus_tag="ACHKA1_005485"
     CDS             <1..1077
                     /gene="cba"
                     /locus_tag="ACHKA1_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin B"
                     /protein_id="HFO5682983.1"
                     /translation="PASQLPAAGNNVPVKYRLTDLMQNGTQYMAIIGGIPMTVPVVDA
                     VPVPDRSRPGTNIKDVYSAPVSPNLPDLVLSVGQMNTPVRSNPEIQEDGVISETGNYV
                     EAGYTMSSNNHDVIVRFPEGSGVSPLYISAVEILDSNSLSQRQEAENNAKDDFRVKKE
                     QENDEKTVLTKTSEVIISVGDKVGEYLGDKYKALSREIAENINNFQGKTIRSYDDAMS
                     SINKLMANPSLKINATDKEAIVNAWKAFNAEDMGNKFAALGKTFKAADYAIKANNIRE
                     KSIEGYQTGNWGPLMLEVESWVISGMASAVALSLFSLTLGSALIAFGLSATVVGFVGV
                     VIAGAIGAFIDDKFVDELNHKIIK"
     gene            complement(1095..1622)
                     /gene="cbi"
                     /locus_tag="ACHKA1_005486"
     CDS             complement(1095..1622)
                     /gene="cbi"
                     /locus_tag="ACHKA1_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin B immunity protein"
                     /protein_id="HFO5682984.1"
                     /translation="MTSNKDKNKKANEILYAFSIIGIIPLMAILILRINDPYSQVLYY
                     LYNKVAFLPSITSLHDPVMTTLMSNYNKTAPVMGILVFLCTYKTREIIKPVTRKLVVQ
                     SCFWGPVFYAILIYITLFYNLELTTAGGFFKLLSHNVITLFILYCSIYFTVLTMTYAI
                     LLMPLLVIKYFKGRQ"
BASE COUNT          532 a          278 c          381 g          495 t
ORIGIN      
        1 cctgccagcc agttaccagc agcgggtaat aatgtaccgg taaaatatcg tctgacggac
       61 cttatgcaga atggtacaca atatatggct attatcggag gtattccgat gacagtgcca
      121 gtagtggatg ccgttccagt tccggaccgg agtcgtccgg gaaccaatat taaagatgtt
      181 tacagtgccc ctgtatcacc aaatctaccg gacctggtat taagtgtggg tcagatgaat
      241 actccagttc ggtctaatcc cgaaatccag gaagatggcg ttatttctga gacagggaat
      301 tatgttgagg ctggttatac gatgtccagt aataatcatg atgtcattgt ccgttttcct
      361 gaaggcagtg gagtttctcc gctatatatt tcagccgtgg agattctgga cagtaatagt
      421 ttaagccagc gccaggaagc cgaaaataac gcaaaggatg acttcagagt caagaaagaa
      481 caagaaaatg acgagaagac ggtcctgaca aaaaccagcg aggtcatcat tagtgtcggt
      541 gacaaagtcg gggaatatct tggagataaa tacaaggcgc tttcccgtga aattgcagag
      601 aatataaata attttcaggg aaaaacgatt cgtagttatg atgatgcaat gtcttccatt
      661 aataagttaa tggctaaccc cagccttaaa ataaatgcaa cggacaaaga agccattgtg
      721 aatgcgtgga aagcatttaa tgctgaggat atggggaata aatttgctgc gttgggtaaa
      781 acgttcaaag cagcagatta tgcaataaag gcaaacaaca tcagggagaa gagtattgag
      841 ggttaccaga ctggtaactg ggggccatta atgctggaag tcgagtcctg ggttatcagt
      901 gggatggcat ctgctgtagc tcttagtttg ttttctttga cattaggctc ggcccttata
      961 gcctttggtc tttcggccac agttgttggt tttgttggcg tagttattgc aggtgctatt
     1021 ggtgcattta tcgatgataa atttgttgat gagttgaatc acaagatcat aaaataatta
     1081 aaaggaaggc agtgttactg cctcccttta aaatatttaa tgacaagtaa tggcatcagt
     1141 aaaatcgcat atgtcatggt taaaacagta aagtaaatgg agcaatataa aataaacaga
     1201 gtgatgacat tatgagataa taatttaaaa aaaccacctg ctgttgttag ttccagatta
     1261 tagaacagtg tgatataaat cagaatggca taaaaaacgg gcccccagaa acaggattgc
     1321 acaacaagtt ttcttgttac tggctttatg atttctcttg tcttatatgt gcaaagaaaa
     1381 acgagaatac ccataactgg cgctgtcttg ttgtagtttg acataagtgt tgtcatgacg
     1441 ggatcatgca atgatgtaat agaagggaga aatgccacct tattatataa gtagtacagc
     1501 acttgagaat atggatcatt tattcgaagt attaatatag ccattaatgg aataatcccg
     1561 attatggaaa atgcatataa tatttcgttt gctttcttgt tcttatcttt attgctggtc
     1621 atatatatac ctttttgtat tttatatata gattatatat cagagttctc aatatatttc
     1681 cagtat
//
LOCUS       DAZFKF010000296         1612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFKF010000296 DAZFKF010000000
VERSION     DAZFKF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.299"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..171)
                     /locus_tag="ACHKA1_005487"
     CDS             complement(<1..171)
                     /locus_tag="ACHKA1_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFO5682985.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFL"
     gene            complement(197..>1612)
                     /locus_tag="ACHKA1_005488"
     CDS             complement(197..>1612)
                     /locus_tag="ACHKA1_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
                     /protein_id="HFO5682986.1"
                     /translation="IIDPGSTASAKVFHAERYGGSGIQRNGGGARCGFDGNYQVKGIG
                     SNPLVGEGTDERHSNGALGAVHAIYEALWGEVLAQILPYSAVRVRAVLLTDLYTEKAF
                     ERSGRKSRRALLVREPVVRPAHFERAPYFQVKPEYSSLLIHDACRVRSVIHKLPGYLP
                     VPPEEIDAEARTDPRIYCIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMD
                     FNGLSCLFPGDSPADFGYKLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARL
                     KVEEIFQKTFHEACYYCYLELLGIPGEFITQKEIPDILKELVNSFVALLNQYCEKSHA
                     QDIVNQDGSPLQKLVVTLIHHRHNQKQALNSSIKNDVHFTVAQQCFSQAIHWLTQGST
                     RRQINASLLLKEIEHHTMKRLQPREELRKENMCEKIAILLDNHGDDPLFLQEAISDMK
                     NFMLKFSRDAFGYLEPIRNTV"
BASE COUNT          461 a          377 c          303 g          471 t
ORIGIN      
        1 caggaattta atctgattat gctgtatcgc atattcagtc cagtattgaa tggttaacac
       61 cggatacaga ctgtaattac gattgtagag catacaactg accgcaaaac cgatctgttc
      121 ataaacagtc cattcatctt ttcctctgat ggtttccctt atataagaca tgattactat
      181 ttcccttata tattttttac actgtgtttc ttatcggttc aagatatcca aatgcatctc
      241 tggaaaactt aagcataaaa tttttcatat cagaaattgc ttcttgtaaa aaaaggggat
      301 catcgccatg attatccagc aggatggcaa ttttttcgca catattttct ttcctcagct
      361 cttccctggg ttgcagcctt ttcatggtat gatgttcaat ttctttcagg agtaatgagg
      421 catttatctg acgtctggta ctgccttgcg tcagccagtg gatggcctgg gaaaaacact
      481 gttgtgcaac ggtgaaatga acatcattct tgatgctact attcagtgcc tgcttttgat
      541 tatgcctatg atggattagc gtcacaacca acttttgcaa tggtgaacca tcctgattga
      601 caatatcttg ggcatgtgat ttctcgcagt attgattgag taatgcaaca aaactgttaa
      661 ccagttcttt caatatatca ggtatctctt tttgtgttat aaattctcca ggaataccca
      721 acagttctag ataacagtaa taacatgctt catgaaaagt tttctgaaat atctcctcaa
      781 ccttcaaacg ggctgcaaga gtgaagtcag ggtcaaacat atattttccg atatacaagc
      841 agagatcaga cagcccttgc ataagtacca tcggttcttt tgccagttca gctaatctta
      901 gtttataccc aaaatcagct ggggaatctc ccggaaacag acaactgagt ccgttaaaat
      961 ccattaatct gccatccatt gcaatattag aaggagaagt tgtcaatctc aggaaacgtg
     1021 ttcgacaaaa tgccatttgc caggcctcac gacgtgccag ttcacataac ccctcaatgc
     1081 aataaatccg gggatcagtt cgtgcttcag catcaatttc ttccggtggt acaggtagat
     1141 atcctggcag cttgtggatc acagatctaa cccgacaggc atcgtgaatt aacagactgg
     1201 aatactccgg ttttacttgg aagtaaggtg cccgttcaaa atgcgccgga cgaacaacag
     1261 gctcacgtac caacagggct cttcgtgatt tcctaccgga gcgctcaaat gccttttcag
     1321 tatagagatc tgtaagtaaa accgcccgaa cccgcacagc actataaggt aatatttgag
     1381 ccagtacttc tccccacaaa gcctcatata ttgcatgaac agcgccgagt gcaccattag
     1441 aatgacgttc gtcagtacct tcgccaacca acggattact tcctattcct ttaacctggt
     1501 agttaccatc aaacccacaa cgtgcaccac ctccattacg ctgaatacca ctgccaccat
     1561 agcgctctgc atggaacact ttggcagaag ctgtactgcc aggatcgata at
//
LOCUS       DAZFKF010000297         1602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFKF010000297 DAZFKF010000000
VERSION     DAZFKF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.300"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..571
                     /locus_tag="ACHKA1_005489"
     CDS             <1..571
                     /locus_tag="ACHKA1_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO5682987.1"
                     /translation="DEAVWVKQALEKNQPGKPVRVINPDDESIRIFSWLADNFPDLQY
                     FKLQLLDASNPRLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISIAVLDDNVLESQA
                     IETLSAIGLSYEKYKTANNVYFNIIGTLSDSELNKINNYVDEYYKQWGKQYVRFNVNL
                     KNQDTNNSSFSYGDNRFEKSQGSKWTFQE"
     gene            585..824
                     /locus_tag="ACHKA1_005490"
     CDS             585..824
                     /locus_tag="ACHKA1_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HFO5682988.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            844..1176
                     /gene="sctI"
                     /locus_tag="ACHKA1_005491"
     CDS             844..1176
                     /gene="sctI"
                     /locus_tag="ACHKA1_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO5682989.1"
                     /translation="MSVSNMPPIDRVEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            1173..>1602
                     /gene="sctJ"
                     /locus_tag="ACHKA1_005492"
     CDS             1173..>1602
                     /gene="sctJ"
                     /locus_tag="ACHKA1_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HFO5682990.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALP"
BASE COUNT          542 a          267 c          332 g          461 t
ORIGIN      
        1 ggatgaagca gtctgggtca aacaagcact ggaaaaaaat caaccaggaa agccagtacg
       61 ggtgataaat cctgatgatg aatcgatacg aattttttca tggcttgctg ataatttccc
      121 tgatttacaa tattttaaac ttcaactatt agatgccagt aatccaagat tgacagtgag
      181 taagcaacgg aacgctatca cacagcaact aatcgataat cttattaaag gattactgca
      241 aactatgcca tatgccagca atataagtat tgcggtatta gatgataatg tattggaaag
      301 tcaggctatt gaaacgttgt cggcgatagg tctttcttat gaaaaatata aaacagctaa
      361 caatgtgtat ttcaatatca ttggtacgtt aagtgacagt gaattaaata aaattaataa
      421 ctatgttgac gaatattata aacaatgggg taaacaatat gtaagattta atgtgaattt
      481 gaaaaatcag gacacaaata atagttcatt tagctacgga gataaccgat tcgagaagtc
      541 tcaaggtagc aagtggacat ttcaggaata attgaggagg acttatggca gattggaatg
      601 gttatattat ggatatcagc aaacaatttg atcagggcgt tgatgatctg aaccagcaag
      661 ttgaaaaagc gttggaggat ttagcaacca atccttccga cccgaagttc cttgccgaat
      721 atcaaagtgc attagctgag tatacattat atcgaaatgc gcaatctaac gttgtaaaag
      781 cgtataaaga tctcgattct gcaatcatac aaaacttccg ctaaactgga taaggatttt
      841 gttatgtcag taagtaatat gccacctatt gatagagttg agcaatcaac tgctcatgaa
      901 atacagcaag cgaaagtgat tgatttaaat gaccgggttc tcaatcttga caacccggat
      961 gataaaatga tctccgcttt tgccaactat gctgtacaaa ctgaaaattg gcagcaaaat
     1021 gcgttacaag cattaaggtc tgataaaaag gggttaactc ctgaaaaatt attagtcttg
     1081 caggatcatg tgcttaatta taatgtcgag gtttcattgg tgggaacatt agcaagaaag
     1141 attgtcgctg cagttgagac gttgacccgc tcatgaaata tatatcatta ttattgttta
     1201 tcctgctgtt gtgcggctgc aagcagcagg aattattgaa ccatcttgat caacagcaag
     1261 ctaatgatgt ccttgctgtt ttacaaagac ataatattaa tgctgaaaag aaggatcaag
     1321 gcaaaacagg attttcaata tttgtcgaac caacggattt tgcttctgcg gtggattggc
     1381 taaaaatcta caatctcccg ggtaagcctg atattcaaat atcgcagatg ttccctgcag
     1441 atgcactcgt ttcatctcca cgagcggaaa aagcaaggct atattctgct attgagcagc
     1501 ggttagagca atctctcaaa attatggatg gcattgtttc ttcgagagta catgttagtt
     1561 acgatgttga tactggcgat agtggtaaaa ctgctttacc aa
//
LOCUS       DAZFKF010000298         1594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFKF010000298 DAZFKF010000000
VERSION     DAZFKF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.301"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1218
                     /gene="mchF"
                     /locus_tag="ACHKA1_005493"
     CDS             <1..1218
                     /gene="mchF"
                     /locus_tag="ACHKA1_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HFO5682991.1"
                     /translation="MDSIMVVGVFVMMLLYGGYLTWIVLGFTMVYVLIRLVTYGYYRQ
                     ISEETLVRGARASSYFMESLYGIATVKIQGMAGIRGTHWLNLKIDAINSGIKLTRMDL
                     LFGGINTFVAACDQVAILWLGASLVIDNQMTIGMFVAFGSFRGQFSDRVASLTSFLLQ
                     LRIMSLHNERIADIALHEKEEKKPEIEIVADMSPVSLETTDLSYRYDSQSAQVFSGLN
                     LSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGKVLVNGTDIRQLGINNYHRMIAC
                     VMQDDRLFSGSIRENICGFAEETDDEWMTECAKASHIHDVIMKMPMGYETLIGELGEG
                     LSGGQKQRIFIARALYRKPGILFMDEATSSLDTESERFVNAAIKKMNITRVIIAHRET
                     TLRTVDRIFRITN"
     gene            complement(1279..1593)
                     /locus_tag="ACHKA1_005494"
     CDS             complement(1279..1593)
                     /locus_tag="ACHKA1_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682992.1"
                     /translation="MTTDELALIHGAGLDKDASVAIGTAAGGFLGKATKIPGADIAGS
                     AIGGYIGGVIADGSNRTITIPSNGINYNPGIGLGSFNPNYNGGLLNSGFNSSTSSSSS
                     GS"
BASE COUNT          461 a          284 c          375 g          474 t
ORIGIN      
        1 atggacagta ttatggttgt gggtgttttt gtgatgatgc tgttatatgg aggatatctt
       61 acctggatag tgctcggttt taccatggtt tacgttctta ttcgtctggt gacatacggc
      121 tattaccggc aaatatcgga agaaactctt gtcagggggg cccgggccag ctcctatttt
      181 atggaaagcc tgtatggtat tgccacggta aaaatccagg gtatggctgg gattcgggga
      241 acacactggc ttaacctgaa aatagatgcg atcaattcag gtattaagtt aaccaggatg
      301 gatttgctct tcggggggat aaatactttt gttgccgcct gtgatcaggt ggcgatttta
      361 tggctgggtg caagccttgt gatcgataat cagatgacaa tagggatgtt tgtggcattt
      421 ggttcttttc gtgggcagtt ttcggatcgg gttgcttcgc tgaccagttt tcttcttcaa
      481 ctgagaataa tgagtctgca taatgagcgc attgcagata ttgcactaca tgaaaaggaa
      541 gaaaagaaac cggaaattga aatcgttgct gacatgagcc cggtttcact ggaaaccact
      601 gatttaagct accggtatga cagccagtca gcacaggtat tcagtggtct gaatttgtct
      661 gtggctccgg gagaaagtgt ggctataact ggtgcctccg gtgccggaaa aaccacatta
      721 atgaaagtat tatgtggact gtttgaacca gatagtggaa aagtactggt taatggcacg
      781 gatatacgtc aacttggaat aaataattat caccgtatga tagcctgtgt tatgcaggac
      841 gaccggctat tttcaggatc aattcgtgaa aatatctgtg ggtttgcaga agaaacagac
      901 gacgaatgga tgacagaatg tgccaaagca agtcatattc atgatgtgat aatgaaaatg
      961 ccaatggggt atgaaacgtt aataggtgaa ctgggggaag gtctttccgg cggtcaaaaa
     1021 cagcgtatat tcattgcccg agctttatac cggaaacctg gaatattatt tatggatgag
     1081 gctacaagtt ctcttgatac agaaagtgaa cgtttcgtga atgctgctat aaaaaagatg
     1141 aatatcaccc gggtgattat tgcacataga gagacaactt taagaacagt tgatcggatt
     1201 tttagaatta caaattagga taatatatca ctaaaagcag cattgctgca taataagcta
     1261 tgctgcttta gaaattaatc aacttccact actagaagag gatgttgatg agttaaatcc
     1321 tgagtttaat aaacctccat tgtagtttgg attaaaactt ccaagtccta tacccggatt
     1381 atagttaata ccgtttgatg gtattgttat tgtcctattt gaaccatcag caataactcc
     1441 tccaatatat cctccaattg cacttcctgc tatatccgca ccagggattt ttgttgcttt
     1501 acctaaaaaa ccaccagccg cagtacctat tgcaacggat gcatctttat ctaatcctgc
     1561 accatgtatt aaagcaagtt catcggttgt tata
//
LOCUS       DAZFKF010000299         1588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFKF010000299 DAZFKF010000000
VERSION     DAZFKF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.302"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(213..1562)
                     /locus_tag="ACHKA1_005495"
     CDS             complement(213..1562)
                     /locus_tag="ACHKA1_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IpaD/SipD/SspD family type III secretion system
                     needle tip protein"
                     /protein_id="HFO5682993.1"
                     /translation="MINKDNNLNIDILRQQNANIPLKEASSQSEEPHQLVNSKSEGNL
                     HNSASKDNNIGAMLKKDVEKLSTLVSERSPRNEEWKCQHDIQLNFVKNMLRNFPTENL
                     SEKGSALLHESYLLIKNVLQLTENVNTIDGKLKDPAKILTLHNAVRKTLANVAHNTTG
                     PGAMATTKGTLTHVIASLDSFAPEMRSSATSSTLTASSSSEFKSDKYICSDIADLMSV
                     LGSDYLEIYANSVEIMSAYWQDFSEHIQSNMGKWTHSNKKGDAIVFDVNAFQKALMHF
                     YYIDKYPNGDFHYHYNPDYVLYPPAPADKIGVPLEEAEKWCAALGLPVIPPDPKHRTP
                     SPIVEVEPQGSGLYVIIPNPQIIDSMSQSSDSMVHRDDKGKEKNISKEFTGYEISTAE
                     YQAWLAGYNGQAENMKTDVQVITTKYSTANSTYDTIIKLLSSTITALFDSAKDYLRF"
BASE COUNT          426 a          305 c          314 g          543 t
ORIGIN      
        1 gcagttgcat agaacgctcc ttccattttc tctattcata gtattaattt gatctgtcta
       61 aaataaagta ttggcagaat tgctctatca aaaaaaactc attttgtgag attgtgatga
      121 agaaatgaat gaaagctgat gcttaataat atgaaacact ggtcccattt tacgggacca
      181 gatagtggac gataattgta ggtgttactt aatcagaaac gtaaataatc tttcgctgaa
      241 tcaaacaacg cagtaatggt tgaagataat aattttataa ttgtatcgta ggtggagttg
      301 gctgtactgt atttagtcgt tataacctgc acgtcggttt tcatgttttc tgcctggcca
      361 ttataacccg ccaaccatgc ctggtattca gccgtgctta tttcgtagcc ggtgaattct
      421 ttagatatat ttttttcctt ccctttatca tccctgtgaa ccattgagtc tgaagactgg
      481 ctcatactgt caataatctg agggttggga ataataacat acagaccgct accttgcggt
      541 tcaacttcga ctataggaga aggtgtgcgg tgttttgggt cgggtggaat aaccggaagt
      601 cctaaagcgg cgcaccattt ttctgcttct tccagtggaa caccaatttt gtcagcaggt
      661 gccggagggt ataaaacata gtctggattg taatgataat gaaaatcacc attaggatat
      721 ttatcgatat aataaaaatg catcaaggct ttttggaatg cattgacatc aaaaactatc
      781 gcatcgcctt ttttattaga gtgagtccat tttcccatat tgctttggat atgttcactg
      841 aagtcctgcc agtatgcgct cattatctca acgctatttg catagatttc gaggtagtca
      901 ctaccgagca cactcatcaa atcagcaata tcactacata tatatttatc acttttaaat
      961 tcgcttgaac tactggccgt taaagtactg ctggttgctg aacttctcat ttcaggcgca
     1021 aaactatcta aagaagcgat aacgtgagtt aaggttcctt tggtcgttgc cattgcacct
     1081 ggcccggttg tgttgtgagc aacgttagcc agggtttttc tcacagcgtt atgcagggtc
     1141 aatattttag cgggatcctt taattttccg tcaatagtat taacattttc tgtaagctgc
     1201 agaacgtttt ttatcagcaa ataactttcg tgtaagagcg cagatccttt ctctgacagg
     1261 ttctcagttg ggaaatttct gagcatgttt ttaacgaagt taagttgtat gtcgtgctgg
     1321 catttccatt cttcatttcg cgggctacgt tcactgacca aagttgatag tttctcaaca
     1381 tctttcttga gcatagcgcc aatattatta tccttactgg cgctgttatg caaatttccc
     1441 tcgcttttgc tgtttaccag ttgatgaggc tcctcacttt gcgaggatgc ctctttgaga
     1501 ggaatgtttg cattctgctg acgtagaata tctatattta aattattatc tttattaatc
     1561 atgagtcatt ccttcttaaa tataatta
//
LOCUS       DAZFKF010000300         1563 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFKF010000300 DAZFKF010000000
VERSION     DAZFKF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.304"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            79..1485
                     /gene="gndA"
                     /locus_tag="ACHKA1_005496"
     CDS             79..1485
                     /gene="gndA"
                     /locus_tag="ACHKA1_005496"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO5682994.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     DNPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGQPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAGNPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLN"
BASE COUNT          395 a          371 c          390 g          407 t
ORIGIN      
        1 gttattgtac attatagtta atttctttaa tatctagcga tatatcaatt aatgactaac
       61 ccctgacagg agtaaacaat gtcaaagcaa cagattggcg tcgtcggtat ggcagtgatg
      121 gggcgcaacc ttgcgctcaa tatcgaaagc cgtggttata ccgtctctat tttcaaccgt
      181 tcccgtgaaa agacggaaga agtgattgcc gacaatccag gcaagaaact ggttccttac
      241 tatacggtga aagagtttgt tgaatctctg gaaacgcctc gtcgcatcct gttaatggtg
      301 aaagcaggtg caggcacgga tgctgctatt gattctctca agccatacct cgataaaggc
      361 gacatcatca ttgatggtgg taacaccttc ttccaggaca ccatccgtcg taaccgtgag
      421 ctttctgcag aaggctttaa cttcatcggt accggtgttt ccggaggcga agaaggtgcg
      481 ctgaaaggtc cttccattat gcctggcggg cagaaagaag cctatgaact ggttgctccg
      541 atcctgacca aaatcgccgc cgttgctgaa gatggccaac cgtgtgttac ctatattggt
      601 gccgatggcg cgggtcacta tgtgaagatg gttcacaacg gtattgaata cggtgatatg
      661 caactgattg ctgaagccta ttctctcctg aaaggcggcc tgaatctctc taacgaagaa
      721 ctggcacaga cctttaccga gtggaataac ggtgaactga gtagctacct gattgacatc
      781 accaaagata tcttcactaa aaaagatgaa gatggtaact atctggttga tgtgattctg
      841 gatgaagcag ctaacaaagg caccggtaaa tggaccagcc agagtgcgct ggatctcggc
      901 gagccgctgt cgttgattac cgagtctgtg tttgctcgtt atatctcttc tctgaaagag
      961 cagcgtgttg ccgcgtctaa agttctgtct ggtccgcaag cgcagccagc aggcgacaag
     1021 gctgagttca tcgaaaaagt tcgtcgtgcg ctgtatctgg gcaaaatcgt ttcttacgct
     1081 cagggcttct cgcagctgcg tgctgcgtct gaagagtaca actgggatct gaactacggc
     1141 gaaatcgcga agattttccg tgctggttgc atcatccgtg cgcagttcct gcagaaaatc
     1201 accgatgcat atgccggaaa tccgcagatc gctaacctgc tgctggctcc gtacttcaaa
     1261 caaattgccg atgactacca gcaggcgctg cgtgacgtcg ttgcttatgc agtacagaac
     1321 ggtatcccgg ttccgacctt cgccgctgcg gttgcctatt acgatagcta ccgtgccgct
     1381 gttcttcctg cgaacttgat ccaggcacag cgtgactatt tcggtgcaca tacttataag
     1441 cgcattgata aagaaggtgt gttccatacc gaatggctga actaatctta atttagatat
     1501 ctcgcccgga ttacaccggg cttttcaatg cctatagaaa accatcagat aggcttgtag
     1561 ttt
//
LOCUS       DAZFKF010000301         1536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFKF010000301 DAZFKF010000000
VERSION     DAZFKF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.306"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..89
                     /locus_tag="ACHKA1_005497"
     CDS             <1..89
                     /locus_tag="ACHKA1_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="effector protein"
                     /protein_id="HFO5682995.1"
                     /translation="TFAAELFDTQNCNKKQNKKPWKKVMFNV"
     gene            82..>1536
                     /locus_tag="ACHKA1_005498"
     CDS             82..>1536
                     /locus_tag="ACHKA1_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO5682996.1"
                     /translation="MFNKVLVGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLS
                     NSFVIIVTCVLALSIGLLPGFPLLVFLCLAVILGIYFYFKFKKKGTEETVVEGDITVG
                     LEPYNQDDDISLGIINKLDQVITETVPLVLIMNSVQAKKYTEINLADRIRSQFFIEYG
                     IRIPGIVIREGEGLNDEDVILMLNEVRASQFKIYHDLVLLVEYSDEVVSTLIKKPVIV
                     NSNGEQYYWVTKSDAQKLTKIGCYTRTAMDEMYNH"
BASE COUNT          438 a          238 c          330 g          530 t
ORIGIN      
        1 taacatttgc agcagagctc tttgatacac aaaactgtaa caaaaaacaa aataaaaaac
       61 catggaagaa ggtgatgttc aatgtttaac aaagttttgg tagggttaag gagtcatcca
      121 gaattaataa ttcttggatt aatggtgatg attatcgcca tgctcataat tccattgccc
      181 acctatttaa tcgacttttt gattgggctt aacttaacat tggcaatact tgtattttta
      241 ggatcattct atgttgatag gatcttaagc ttttcttcat ttccatcaat cctccttatc
      301 acaacgttat ttcgtttggc gctggcgatc agtaccagtc gacttatact tcttgaggcg
      361 gatgctggag aaattataac gagttttggt gagttcgtta ttggagatag cctggtagtt
      421 ggttttgtta ttttctccat tgttactatt gttcagttta tcgtgattac taaaggttcg
      481 gaacgtgtgg ctgaagttgc tgcccgcttt tcgcttgacg gtatgccagg taaacaaatg
      541 agcattgatg ccgatctacg cgctgggatc attgatgcag atttagcaaa agaacgtcgt
      601 agcgtattag aacgagagag tcagctgtat ggttcctttg atggtgcgat gaaatttatc
      661 aaaggtgatg ctattgcaaa cattattatt atctttgtta atattatcgg tgggctatca
      721 gtaggagtag gccaaaatgg aatggatttc tcaacagcgt tgactgtcta cacaattctt
      781 actgtcggtg atggtcttgt ttctcaaatt ccagctttat taattgctat tagcgcagga
      841 tttatcgtta cgcgcgtaaa tggcgatagt gataatatgg ggcaaaatat aatgtcccag
      901 ttattaagta attcttttgt cattattgtg acatgtgtcc tcgcgctaag tattgggtta
      961 cttccaggtt ttccccttct tgtgttttta tgtttggcag tgatcttagg aatttacttt
     1021 tactttaaat tcaaaaagaa agggactgaa gaaacggttg ttgagggaga tattaccgtt
     1081 gggttggaac cctataatca agatgatgac atctcattag gtatcattaa taaacttgat
     1141 caggttatta ccgaaactgt acccttggtc ttgattatga atagtgtaca ggctaaaaaa
     1201 tacactgaaa tcaatcttgc tgataggatt cgtagtcagt tttttattga gtatggtatc
     1261 cgcattcccg gtattgtcat tcgtgagggg gaaggtctta atgatgaaga tgtcatatta
     1321 atgctaaatg aagtcagagc ttcgcaattt aaaatttatc atgatcttgt cttgttggtt
     1381 gaatattcag atgaagtggt ttcgactttg atcaagaaac ctgtcattgt aaatagtaat
     1441 ggtgaacaat attattgggt tacgaaaagt gatgctcaaa aattaacaaa aattggctgt
     1501 tatactcgca cggccatgga tgaaatgtat aatcat
//
LOCUS       DAZFKF010000302         1530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFKF010000302 DAZFKF010000000
VERSION     DAZFKF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.307"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            557..748
                     /locus_tag="ACHKA1_005499"
     CDS             557..748
                     /locus_tag="ACHKA1_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5682997.1"
                     /translation="MQAASQREETEWRVQSKRGLMPAYRGEAGQQVNINIMEYSERNV
                     RQLTLNEQEDTSPGKLMLV"
     gene            complement(818..958)
                     /gene="ykgO"
                     /locus_tag="ACHKA1_005500"
     CDS             complement(818..958)
                     /gene="ykgO"
                     /locus_tag="ACHKA1_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO5682998.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(958..1221)
                     /gene="ykgM"
                     /locus_tag="ACHKA1_005501"
     CDS             complement(958..1221)
                     /gene="ykgM"
                     /locus_tag="ACHKA1_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO5682999.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
BASE COUNT          454 a          304 c          328 g          444 t
ORIGIN      
        1 ttttttgata agaagaaaaa gacattcagt ttcataaggt tattggcaat ataacgttca
       61 attcttactt gcattttata ttagccagga actttgccct gaggtgttag gaatagtctt
      121 aatgtgttgt gtaatggact agtaaaaaaa caagtttaaa tgacaagaaa tagaatataa
      181 aactatcatg agtcataatt aaagatgatt tgccaatcag taaggtgttt taatggatta
      241 acatgccgca caaaatggtt atgggtgttc atcccaataa gtgatgaaaa attaaacagg
      301 tgtaagtacg tcatctgtcc ggtaagcccc ctaagagcca aattaagtga agttaaacga
      361 agactgccag ttgtcacagg aacgatggcc tctttgccag ggcactgcat tcagtcatct
      421 gtgcgcgtta ctctaactgg cactcgaacg tcggcgacgg ggttaccggt ggatcaggcc
      481 gttcctgtgg cgtaaacgtt tttatgtcag taccaacaga tttaccacat ttatcatctt
      541 aacagtttga gtgtaaatgc aggctgcgtc gcaaagggag gaaactgagt ggcgtgttca
      601 gtctaaaaga gggttgatgc cagcatacag gggcgaagct ggtcaacaag tcaacattaa
      661 tataatggaa tattcggaga gaaacgtcag gcagttgacc ttaaatgaac aggaagatac
      721 atccccagga aaattaatgc tggtgtaata aatacaccaa cattaatccg gtctgattat
      781 taatcagaca aatatcgtta atccgtcgaa ttttgaatca acgttttttc ttacgaccct
      841 gaacggcctt aaaacgtgga ttagatttac aaatcacata tagccgtcct tttcgcttca
      901 caatctgaca gtctggatgg cgttcttttg cggtacgcag agagttaagg actttcatca
      961 cgcccccttt ttcgtgctaa caaaacgacc aaaacgttgg gtgaatcgtg caacatttcc
     1021 ttctgatgcc actgttctca gcttccctgt atagaacggg tgcgatttcg aagagacatc
     1081 aattgttacg tacggatatg ttacgccatc cagctcaatc tcacggtctg ttttgatagt
     1141 cgagccgatt ttaaagtact catcaacact ggtgtcgtgg aacaccacag tacgatactc
     1201 aggatggata ttgggcttca tcatttttac ctgttatgtt ataacataac cataaagata
     1261 catgctcatc cggatcattt caacacccaa tccctgtgac ccttagtggt atgtggaatt
     1321 tcctcaacgg ggaagtctgg cattgttaac agccattggc gacgctccca taaacgcctg
     1381 gatgagtgat tgatgatagt ttcgctgaga gtatggattt ttcgagaatg gaggttatct
     1441 ccactttact ggccgatgat ttatcgcagt atgattgatt acctgacagc gtgtattcct
     1501 ctcccacgga tataataatt acttacaaaa
//
LOCUS       DAZFKF010000303         1502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFKF010000303 DAZFKF010000000
VERSION     DAZFKF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.308"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..439)
                     /locus_tag="ACHKA1_005502"
     CDS             complement(<1..439)
                     /locus_tag="ACHKA1_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5683000.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAM"
     gene            complement(439..768)
                     /locus_tag="ACHKA1_005503"
     CDS             complement(439..768)
                     /locus_tag="ACHKA1_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5683001.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(765..>1502)
                     /locus_tag="ACHKA1_005504"
     CDS             complement(765..>1502)
                     /locus_tag="ACHKA1_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5683002.1"
                     /translation="AQVTGALKQTWADEDMLRGDWLAGLKSGWGEWTESATDSFSQVK
                     SAATQTFDGIAQNMAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGG
                     AFGGGASASTGTAIEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGV
                     GNLYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGINGQAGPQLMKAV
                     YDMARKGAQDELRLQLRDGGMLSGSGR"
BASE COUNT          304 a          556 c          329 g          313 t
ORIGIN      
        1 tcatggccgt cagcgatgac atctgctcca ccacccaccg gtccgtcagc tcctgctccg
       61 ggtcggcctc aggattgcct gccacaaaat tcaccgcatc cagaaaacgc gcatacaccc
      121 ggcgacggac caccgtggca cccaccaggc tctgcaaatc ctctgccatc ccggtgacca
      181 gcccgaaaag attggacact gtcagcgacg ggcgggcact gctgcccttt ccgttcatct
      241 caaagccgct gccctcaatc gggtacgcct gatattcacg cccctgccag gtcactggct
      301 cccctttttc attcagctca ttgcagaaaa aataccgctc accgccctgc accgtcaggt
      361 cgatttccca gagcaccacc cgcggtgact gctctgattt aaccgactcg ttcaggcttt
      421 cttcgtgaat atcctgcatc agttcaccac ctgcttaaac tccgcgctga actcaacgcg
      481 caacatcccg acccgcgcag accacccggc acaggtcacc tttatctgcc ggtatgcata
      541 gggtggcttc cacaaaaatg ccttccagcc cccgtgctct gccaggaacg cttccagatg
      601 ccgggcctcc tcccgggtca cggaaagcgt caccctgtat gttttcaggt cagcattcag
      661 ccctgccgcc atacgctgtg agtacccgtc accaaaacgc acttcacgca ccgacggctg
      721 cgagttcacc tccatatccg gcttcacttt ccagcgaaag gttttcatcg cccgctccct
      781 gataacatac cgccatcacg caactgcagc cggagctcat cctgtgcccc tttacgggcc
      841 atgtcataca ccgccttcat cagctgcggc cccgcctgtc cgttgatacc gtcgttctga
      901 atcaccacgt gattgttctg attaaaatta ataccttccg cccgccgcat ctgcgccgga
      961 cttccggcac cgccgacata accaccttcc gcatagccgc gcatcaggcg gtaaagattc
     1021 cccacaccta tccggctggt tgcctctttc gtgaaaacaa actccccgcg gtgaactatc
     1081 cccgcaggct catatttgcc gcccgtcccc gtaaatccgc cggtcgcaaa atgaaagttc
     1141 gccgccgcag cctcaatggc cgttcccgtg gaagcggatg cgccaccacc gaaagcacca
     1201 ccaatggcgc tgccgatacg cccgacaatt cccaccatgg cctgtttaag caggatttct
     1261 gtcatcatgg acagcaccga acgggtgaat ccccgccagt ctgcctctgc accggtcagc
     1321 atcgccgcca tattctgtgc aataccgtca aaggtctgcg tggcagcact tttaacctgc
     1381 gaaaaactgt ccgtcgcact ttccgtccac tccccccagc cggacttcag cccggccagc
     1441 cagtcaccgc gcagcatgtc ttcatccgcc catgtctgtt tcagtgcccc ggtgacctgt
     1501 gc
//
LOCUS       DAZFKF010000304         1501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFKF010000304 DAZFKF010000000
VERSION     DAZFKF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.309"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            141..1244
                     /locus_tag="ACHKA1_005505"
     CDS             141..1244
                     /locus_tag="ACHKA1_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683003.1"
                     /translation="MNSYIKIEQTGSNKFKVTDMYREFNDETKCFDKKCRDMDFSGRN
                     VKEFIRLLSDHDQFEKDQISELTVAANALKLEVAKNNYNMKYSFDRQTERRMIELIRE
                     QKDLIPEKYLHQSSIKNLKLHEDEFSSLLADTERQVLEGSSFVLCCGEKINGTISELL
                     RKKITDSTHSIKSLTLSESPSYEVYEEIFKKAVFTPDECHLVRDSLVQDGIKPEKIAK
                     IISCLSSRTGIGNLFYACSQAFHVANDNAALDVRRAATSVGDTIDVCPSLVALNGRSY
                     HQHVDFSEYGFTQTLCMLGAVVNNANDTFDVANYIYTTKGENIIDNDAEDDSPYVHSD
                     ESYRHKDDYWARDNDVRNLRRNFSGNITIATYK"
     gene            1406..>1501
                     /locus_tag="ACHKA1_005506"
     CDS             1406..>1501
                     /locus_tag="ACHKA1_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="response regulator in two-component regulatory
                     system wtih UhpB; phosphorylated UhpA is a positive
                     activator uhpT, a hexose phosphates transporter; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO5683004.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAE"
BASE COUNT          481 a          272 c          289 g          459 t
ORIGIN      
        1 tttttcgaag ttttttatta tatcttttag attcatttca tatctatcgg catgatatta
       61 gtgataaatt ggttccttat tttcatgtta cgcctgaatc ggagaatgcg agttataaga
      121 catggattgc cgttacaggc atgaatagct atataaaaat tgagcagacc ggaagtaata
      181 aatttaaagt cactgatatg tatcgtgagt ttaatgacga aacaaagtgt tttgataaaa
      241 agtgtaggga tatggatttt agtggtcgaa acgtaaaaga gtttataagg ttattatcag
      301 atcacgatca atttgaaaaa gatcaaatct cagaattaac agttgctgca aatgctctta
      361 agctggaagt tgctaaaaat aactataaca tgaaatactc ttttgatagg caaaccgaac
      421 gccgaatgat agagcttata agggaacaaa aagatttaat tccagaaaag tatttacatc
      481 aatcatctat aaaaaacctt aagcttcatg aggatgaatt tagctcactc cttgcagata
      541 cagaaagaca agttctggag ggaagttcat ttgttttatg ttgtggtgaa aaaataaacg
      601 gcactataag tgaattactt cgcaaaaaaa ttacagattc aacacattcc atcaaatctc
      661 tcactctctc tgaatctcct tcttatgaag tttatgagga aatattcaag aaagctgttt
      721 tcacacctga tgaatgtcat ctggtgagag attctttagt gcaggatggt ataaagccag
      781 aaaaaatagc aaaaattatt tcttgtttat ccagtcgtac cgggattgga aatttgtttt
      841 atgcctgttc acaagcattt catgttgcta atgataacgc tgcacttgat gtgcgtcgcg
      901 ccgctacatc tgttggcgat acaatagatg tttgtccgag tttggttgct ttgaatggta
      961 gaagttacca tcagcatgtc gatttctcag aatacggatt cacacaaacc ctttgcatgt
     1021 taggagcagt cgtaaataac gcgaatgaca cctttgatgt tgctaattat atctatacca
     1081 ctaagggcga aaatattatc gataacgacg ctgaagatga ttctccctat gtacattccg
     1141 atgaaagtta tagacacaaa gatgattatt gggcaaggga taatgatgtc cgcaatctaa
     1201 ggagaaattt ctcaggcaat attacgattg caacctacaa ataattcgct ttttctcaat
     1261 aacacgcacc aaacgcttac ctttcaggta agcgttttga cgataagccc aacatcattc
     1321 agccaataca taccccatat ccaacttgaa caacatcgcg cttatcgtta aggtatgcgc
     1381 gttttttatc cgccaggacc caatcatgat caccgttgcc cttatagacg atcaccttat
     1441 cgtccgctcc ggctttgcgc agttgctggg gctggagcct gatttgcaag tagttgccga
     1501 g
//
LOCUS       DAZFKF010000305         1495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFKF010000305 DAZFKF010000000
VERSION     DAZFKF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.310"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            147..403
                     /locus_tag="ACHKA1_005507"
                     /pseudo
     CDS             147..403
                     /locus_tag="ACHKA1_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(425..696)
                     /locus_tag="ACHKA1_005508"
                     /pseudo
     CDS             complement(425..696)
                     /locus_tag="ACHKA1_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206187.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(693..>1495)
                     /locus_tag="ACHKA1_005509"
     CDS             complement(693..>1495)
                     /locus_tag="ACHKA1_005509"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HFO5683005.1"
                     /translation="ISYRCLRYGMKVLGTQSTGVRLGFETGFDSGSTLDYIYRNQPQG
                     STSLGRLIDKNYLNSVGWKGIRQRKIHLQMLIQQAVARLTEQGSSVRIVDIAAGHGRY
                     VLDALQGETAVSEILLRDYSELNVAKGQEMIASRGMAGRARFEQGDAFNRNELAALEP
                     RPTLGIVSGLYELFPENGPVRDSLAGLAAAIEPGGILIYTCQPWHPQLKTIAWSLTSH
                     KNGKAWVMRVRTQGEMDALVRDAGFEKCTQLIDEMGIFTVSMAVRRQP"
BASE COUNT          386 a          391 c          364 g          354 t
ORIGIN      
        1 acagaagtaa attaatttga agtaaataaa gcaaaatatc agaagaatat atcggggcgc
       61 acagtctatc tctgcctttg cttcatggga tgttctttgt atattttact tgctgtttaa
      121 gtatgtatat aaggagtgca ttcattatgc caatgaagct tgtaaagaag aatccccgga
      181 gcaaagtgat tcgggagctt agctgtgagc aagagatggc agtaaaggcg gtctgcggac
      241 tgaaaaaaca gcaactacgg cactacatgc cttggccaat gatcttctcc gggaaatgcg
      301 tgagtatgat gtatggttac agtgtgactg tattccggga gattcgccga ccatgaattt
      361 tgctgctctt aaaaacaaca cgggaacttt ttatctgtcc agtttggata tagataatta
      421 ttgtactaat aagggaagcc aacaccagcc gacagaccag acgccgctgc tcattcagtg
      481 aaacgcagat aaacagtaaa gcaaatccag actccagtac ggaataatcg tcagcggtat
      541 taacgggatc cggtgctccc agtcaaacac caggctgctg atgttttctc tcccggcggt
      601 aaactgattc gtcatgccat aagtaagaaa gaagaatggg gccagcacca ttagccagcc
      661 gattccctga ataatcaccg cacgccgcgt tgtcatggtt gacgtcgtac cgccatagag
      721 accgtgaaga tgcccatttc atcaattagt tgcgtgcatt tctcaaaacc ggcgtcacgg
      781 accagcgcgt ccatctcgcc ctgagtacgt acgcgcatta cccaggcttt gccatttttg
      841 tgactggtga gtgaccaggc gatggttttt aactgagggt gccagggctg acaggtgtag
      901 atcagaatgc ctcccggttc aatcgctgca gccagcccgg caagcgaatc tcgtaccggc
      961 ccgttttccg ggaacagttc atacagaccg gacacgatcc ccagcgtcgg acgcggttcc
     1021 agtgccgcca gttcattacg attaaaggca tccccctgct caaatcgcgc acggcccgcc
     1081 ataccgcgag aggctatcat ctcctgacct ttcgccacgt ttaattcgct gtaatcgcgc
     1141 agaaggatct cgcttaccgc cgtttcgcct tgcagggcat caagcacata gcgtccatgc
     1201 ccggcggcaa tatccacgat ccggaccgag ctaccctgct ctgttaaccg agcaacagcc
     1261 tgctgaatta acatctgcag atgaatttta cgctggcgga tccccttcca gcccacgcta
     1321 ttcaggtagt ttttatcaat gaggcgtccc aacgaggtgc tgccctgagg ctggttgcga
     1381 tagatgtagt ctagggtact gccggaatcg aaaccagtct caaatcccag acgcaccccc
     1441 gtggactgcg tgcccagcac tttcatcccg tagcgcaaac agcggtaaga gatat
//
LOCUS       DAZFKF010000306         1465 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFKF010000306 DAZFKF010000000
VERSION     DAZFKF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.311"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1181)
                     /gene="rhsD"
                     /locus_tag="ACHKA1_005510"
     CDS             complement(<1..1181)
                     /gene="rhsD"
                     /locus_tag="ACHKA1_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO5683006.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGIFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQVQRAEEARTSSLSSSDRSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLVRYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYCYQYEQDRIT
                     VT"
BASE COUNT          306 a          457 c          395 g          307 t
ORIGIN      
        1 gtgacggtga tgcggtcctg ctcatactgg tagcagtaac tcaggcctgc cgggtttagt
       61 tgctccacca cccgccccgc atcgtcgtag cggtagcgca tctccggcct tcccgcgtaa
      121 cggtgtgcca ccatccgccc ggggtgctgc gcgtcatatg tgaaagcgcg cacctgcgta
      181 ttgctgcggt catataccgc cagcagttca ccggcctccg tatacgtgta ccgcaccagg
      241 ggcgcaccgg gcaggctctc cgggtacgcc gggtcgtgca tcagccacac cgccgaaagg
      301 cggatacccc tgtcggggcc gtattcggta ccgggcagtg tgtcggggaa cgctgaggct
      361 gagagagggc gggaacggtc agaagaagat agcgaagagg tgcgggcctc ttccgcacgc
      421 tgcacctgcg tggtcagcac cagacggaac tcccgcccgg caccgtccgt cacgccggtg
      481 atttccccgg ccaggtcacc ggcggcctca cgccggtacg tcagcgtccg cccgaagcgg
      541 tccgccatcc cggtaagcac ccggtacggc ggcagcggcg ctggcagtac gtcctcagca
      601 cccggcaccc gctcagacca ccccagtatc caccacggcc cctgtgcgct gttggtcgcc
      661 aggtaaagat gcgggcttaa ccggatatcc ggcggcagcg acgcccacag ccgtgccagc
      721 gtgtggccgt ccggctgtgc tgccttacca ccgcgcacca gccacattga ctcgctgcgg
      781 ctgtacaccg cctcacccgg cagcagcggc tcaaagtgaa tgctccgtcc gccgttgtcg
      841 ttgagtacca gtgcatcatc gcgtagctgt aagcggatat cagaaggcgc tttccagccg
      901 gggccaaaaa tccccaccgg tgcaggcgtc ttcgtccggt agctgctgta ggtgcgggag
      961 agaatgaacg gcagcgggcc gggcagcgca aggtccgtct cgccgggcag caccttcgcc
     1021 cccagcagcg gatttaccgg gttgcccgag gtcatcccgc ccggacacac cgagcacgcc
     1081 acgccagttg gcgcgccaat tcttacacct gccgaaccct ggacaatggg accgccatac
     1141 tgagtcatat ctccctgacg cgccgctggt tttccgctca tataaagtgc ccttttagtt
     1201 attgttcata tccctgtgtt agcaacaaaa aaaataaaat gacacacaag accagctaat
     1261 gtatttatat atttttcaca taaacttatt tgttctcaat caacaacaac tgttattaca
     1321 ggtcactttc aatattttat taaactaaaa tttttagcac agatgaattc atcatcaaca
     1381 aattcactat atggataata tccatttaac gcatatgatt tatgatttta ttagtctgag
     1441 ttatttttgt agggctatat ttaaa
//
LOCUS       DAZFKF010000307         1463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFKF010000307 DAZFKF010000000
VERSION     DAZFKF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.312"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..75)
                     /locus_tag="ACHKA1_005511"
     CDS             complement(<1..75)
                     /locus_tag="ACHKA1_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683007.1"
                     /translation="MNHEEMNQRFSRLENEIAELNKKLS"
     gene            complement(79..294)
                     /locus_tag="ACHKA1_005512"
     CDS             complement(79..294)
                     /locus_tag="ACHKA1_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5683008.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            complement(370..642)
                     /locus_tag="ACHKA1_005513"
     CDS             complement(370..642)
                     /locus_tag="ACHKA1_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5683009.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLEACLDAEVDA
                     ILDELLGVPAEPPTEAGDTTAESGELQPESPVSDATEPQPESVMML"
     gene            complement(668..850)
                     /locus_tag="ACHKA1_005514"
     CDS             complement(668..850)
                     /locus_tag="ACHKA1_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5683010.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            complement(990..>1463)
                     /locus_tag="ACHKA1_005515"
     CDS             complement(990..>1463)
                     /locus_tag="ACHKA1_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5683011.1"
                     /translation="GQGAELLKKGGRVSVRFKVPGALVPNQFALGIYWQLSSLPEGVT
                     LSEEGNDMLMSFFLQTDATNLNAMYHKKQNAKLDTFGVFDNGWHTLAFEFAGNNSIQV
                     TPVLDEKRGTPFTLVKSPASGAADKLQLTDISKAAAYTLLIDSVKVEVNNADVAA"
BASE COUNT          313 a          441 c          329 g          380 t
ORIGIN      
        1 cgacagtttt ttattcagtt cagcaatttc attttccagg cgactgaagc gctgattcat
       61 ttcttcatgg ttcattattc actctccccg ggcggcctta cgccggtctt ctttaatttt
      121 gaaatacagg ttcgtcaggt acgtcagcag cccaaacagc agactcccca gcacacctat
      181 tgccgcccac tgagacgggg aaaccctgtc cagcaactgc aggaaccagt agcccgttcc
      241 caccgctgac gtggtgtatg acacacctgt tgtgattttt tccatctggt acataccccg
      301 tctcccgcac acggaagctc acaacatgaa aaaggccagc agcagtttac tgatggcccc
      361 gacaccccgt tacagcatca tgaccgattc gggttgcggt tcagtcgcat cggagaccgg
      421 tgattcaggc tgaagttcac cgctctctgc ggtggtatct cccgcttcag tcggtggctc
      481 tgccggtacg ccaagcagct catccagaat ggcatcaact tctgcatcaa gacacgcctc
      541 aaggttctgg cggagtttct gtttcagtgc gctcaggact tcttcagagc gcaggacttc
      601 cttcactgcc tctgcagtga ccagggatgt gatttctgac atgggatttt ctcgctgaaa
      661 ggggttgtta aggagtaacg ggctcttcgg gtttgcttcc ggctgactga ctggcgctga
      721 ttttctctgc ggccctttta tcaatctgcc tgcgccagaa atcgcgcact gccctgtacc
      781 cacccgaaag gagatacagc acacagaccg ccgtacagaa gtacaacatc acctgatgaa
      841 taaatgtcat aatttcttac cgttatggtt gacaatgaga actgttttca ttaaaaaaaa
      901 caaccgatgt acgaaagcat cttttcttta cattctccat tgggattacc tccgccagct
      961 tccattcctg ccgctggcgg ctttttttat catgccgcga cgtccgcgtt gttcacttcc
     1021 accttcacac tgtcaatcag cagcgtatat gccgccgcct ttgatatgtc tgtcagttgc
     1081 agtttgtccg ccgcccctga tgccggagat ttcaccagtg tgaacggtgt accccgtttc
     1141 tcatccagta ccggcgtcac ctgaatgctg ttgtttccgg caaactcaaa agccagtgtg
     1201 tgccatccgt tatcaaaaac cccgaacgta tccagcttcg cattctgctt cttgtggtac
     1261 atcgcgttca ggttcgtcgc atccgtctgc aggaagaagg acatcagcat gtcgttgcct
     1321 tcctctgaca gcgtcactcc ctccggcagg gacgacaact gccagtaaat acccagggca
     1381 aactgattcg gcaccagtgc gcccggcacc ttaaaccgca cgctcacacg tccgcctttc
     1441 ttcagcaact ctgccccctg ccc
//
LOCUS       DAZFKF010000308         1428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFKF010000308 DAZFKF010000000
VERSION     DAZFKF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.313"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            24..>1428
                     /locus_tag="ACHKA1_005516"
     CDS             24..>1428
                     /locus_tag="ACHKA1_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5683012.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNDPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFIPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKRDLVDYNIINELLFNCSKIQPVENKKDANETLELKIIELQMKINNLI
                     AALSVAPEVTAIAEKIRVLDKELRRASVSLKTLKSKTVSSLGDFHAIDLTSKNGRELC
                     RTLAYKTFEKIIINTDNK"
BASE COUNT          493 a          212 c          307 g          416 t
ORIGIN      
        1 agcattcgtt agcaggtgtc atcatgaaaa aagccatagc atatatgcga ttttcatcac
       61 caggtcagat gtctggcgac tcattaaacc gacagagaag acttattgct gaatggttaa
      121 aggtaaatag tgattattat cttgatacca taacatatga agatttagga ttaagtgcat
      181 tcaaaggaaa gcatgcacaa tcaggagctt tttcggaatt tttagatgct atagagcatg
      241 gttatatatt gccaggaact acattgttag ttgaaagtct ggacagactt tcaagagaaa
      301 aagtcggtga agcgattgag cgtctgaaat tgattttgaa tcacggtatt gatgttataa
      361 ctctttgcga taatacagtc tataatattg actctttgaa tgatccatat tcattaataa
      421 aagccatact tatagcacaa agggcaaatg aagaaagcga gataaagtca agtcgggtta
      481 aattatcatg gaagaaaaaa cggcaggatg cactggagtc aggcacgatt atgacggcgt
      541 cttgtccgag atggctctca ttggatgaca aaagaacagc ttttattcca gaccccgaca
      601 gggtgaaaac gattgagcta atttttaaac tcaggatgga aaggcgatca ttgaatgcaa
      661 tagccaagta tttaaatgat catgctgtaa aaaatttctc aggaaaagaa agtgcatggg
      721 ggccttctgt aattgaaaaa ttattagcga ataaagctct gataggtata tgcgtacctt
      781 catatcgtgc aagaggtaaa ggaataagtg aaatcgctgg ctattatccc agagtcatat
      841 cagatgattt gttttacgct gtgcaggaaa ttcggttggc accttttggt attagcaata
      901 gtagcaaaaa tcctatgttg ataaatctac ttcgaacagt tatgaagtgc gaggcttgtg
      961 gtaataccat gattgttcat gcggtatctg gaagtttgca tggctattat gtttgtccga
     1021 tgagaagact gcatcgatgt gacaggccat caataaagag agatttggtt gattataata
     1081 tcattaatga gttgcttttt aattgtagta aaatccaacc agttgaaaac aagaaagatg
     1141 ctaatgaaac tttagagttg aaaattattg agctccagat gaaaattaat aatttaattg
     1201 ctgcattatc tgttgcgcct gaagttaccg ctatagcaga aaaaatcaga gtattagata
     1261 aggaattacg aagggcttct gtatcattaa aaactttgaa gagtaaaacg gtgagctcac
     1321 ttggtgattt tcatgctatt gacttaacca gtaaaaatgg gcgagagcta tgtcgtacac
     1381 ttgcctataa aacattcgaa aaaatcataa tcaatacaga taataaaa
//
LOCUS       DAZFKF010000309         1404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFKF010000309 DAZFKF010000000
VERSION     DAZFKF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.314"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..758
                     /locus_tag="ACHKA1_005517"
     CDS             <1..758
                     /locus_tag="ACHKA1_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5683013.1"
                     /translation="AATPKAVKAAMDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYG
                     RDIHTGAGEWKTREFVAWLKEKGAFDQPYWMMKASLLASMNKVITDVGPGKLNLGGCV
                     IEVMGTYEAAIVRVTIGEYGATGFINGTVCTCTVYGDTQRFHWRVDYSTKNKPTTASL
                     TVNGWERDEVTGRLRQWGSIEVSEDDGKLMTQHSIRFPVAFPVAALNAQVSAVGDPHA
                     IKMYTLSNPSLVSVTLITVRGCYGKFSWEAIGY"
     gene            768..992
                     /locus_tag="ACHKA1_005518"
     CDS             768..992
                     /locus_tag="ACHKA1_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5683014.1"
                     /translation="MEQYVFSPSENMFYPLSLRPVYEAAGRWPEDGIVVDYVVYKVFA
                     ADAAPAGMKRGVGAEKMPVWVPVSEEGTET"
     gene            992..>1404
                     /locus_tag="ACHKA1_005519"
     CDS             992..>1404
                     /locus_tag="ACHKA1_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5683015.1"
                     /translation="MHIRNFSYYTPAEPDVAGAMYLKSEDGQDWYECQALFSPETLKV
                     VYDSRGVITGYGKDTALLWPVNQSVAEVPDTPESRKIDLSGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKST"
BASE COUNT          352 a          287 c          461 g          304 t
ORIGIN      
        1 aggcagccac cccgaaagcg gtgaaagcgg caatggacaa agcggacgga tgcctggaga
       61 aagcgaaaaa cggtgacgat atcccggata aggtgaagtt tctgaacacc gtgggagcag
      121 ccagagtata cgggcgggac attcatacgg gggccggtga atggaaaaca cgcgagtttg
      181 ttgcctggct gaaagaaaaa ggggcatttg accagcctta ctggatgatg aaggcatcac
      241 tgcttgcgtc aatgaataaa gtcatcacgg atgtcggacc gggaaaactc aatctgggtg
      301 gctgtgtcat tgaggtgatg ggaacgtatg aggctgccat agtccgggtc accattggcg
      361 aatacggtgc aacggggttt attaatggca cggtctgtac ctgtacagtt tacggagaca
      421 cacaacgttt tcactggcgg gtggattaca gcacaaaaaa caagcccaca acagcaagcc
      481 tgacagtaaa tggctgggag cgtgatgaag taaccggtcg tttgcggcaa tggggcagta
      541 ttgaggtaag tgaggatgat ggtaagctta tgacacagca ctctatccgt tttcctgtcg
      601 cttttccggt tgctgctctg aatgctcagg tgtctgcagt gggagacccc cacgccatta
      661 agatgtatac cctgtcgaat ccgtcattag tgtctgtgac tctgataaca gtcaggggat
      721 gctacggcaa gttttcatgg gaagcgatcg gatactgagg gcattgtatg gaacagtatg
      781 ttttttcacc gtcagagaat atgttctatc ccctgtccct gcggccggtt tatgaggcag
      841 cagggcgctg gccggaagac ggtattgtgg tggattatgt ggtgtataaa gtctttgcag
      901 cggatgctgc accggcaggt atgaagcggg gcgtcggtgc ggaaaaaatg ccggtctggg
      961 tgccggtatc ggaggagggc acggaaacat gatgcatatc agaaattttt cgtattacac
     1021 cccggcggag ccggatgttg caggcgcgat gtacctgaag tctgaggatg gtcaggactg
     1081 gtatgagtgt caggcgttgt tttcgccgga gacgctgaag gtggtgtatg acagccgggg
     1141 tgtgattacg ggatacggta aggacactgc gttactgtgg ccggtgaacc agagtgtggc
     1201 agaagtgccg gataccccgg agagccggaa aattgacctg agcgggcgct ggggttttga
     1261 cggggagaaa atcacggacc tgctgaccgc ggagaaagcg cgagggatga agggcgatga
     1321 aattaacgcc tggcgtaatg cgatggaagc ggctaactac acgtttgagc acaatgggcg
     1381 taaatgggac tacgggaagt caac
//
LOCUS       DAZFKF010000310         1347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFKF010000310 DAZFKF010000000
VERSION     DAZFKF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.315"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1181
                     /locus_tag="ACHKA1_005520"
     CDS             <1..1181
                     /locus_tag="ACHKA1_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO5683016.1"
                     /translation="ALLMEASTWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFR
                     RGLGNGVFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIA
                     VMTLFNSLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSC
                     IGITFLAYAGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALE
                     LEKYADTAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSERELP
                     KLMNKSLWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRH
                     DIHASLPILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV
                     "
BASE COUNT          257 a          323 c          364 g          403 t
ORIGIN      
        1 ctgcgttgct aatggaagcc tcgacctggg tcgcctttgc ttttggcggt attgtggcga
       61 tgttttccgg ttatgcctat gcgcgtctgg gggcgagcta tcccagcaat ggcggcatta
      121 tcgacttctt tcgtcgcgga ttaggcaacg gcgtcttttc gctggcgctc tcgttactgt
      181 acctgttgac gctggcggtg agcatcgcca tggtcgcccg tgcttttggc gcttatgccg
      241 tgcagttttt gcatgaaggc agccaggagg agcaccttat tttgctctac gcgttgggga
      301 tcattgcggt gatgacgctt ttcaactcct taagcaacca tgcggtaggg cggctggagg
      361 tgatcctcgt cggcattaaa atgatgatcc tgttattgct gattattgcc ggtgtctggt
      421 cgctgcaacc ggcgcatatt tccgtctctg cgccccccag ctccggtgcg ttcttctcct
      481 gtatcgggat aaccttcctt gcctatgcgg gctttggcat gatggcgaac gcggcggata
      541 aagtgaaaga tccgcaggtc attatgccac gggcgtttct ggtggcaatt ggcgttacca
      601 cgttgcttta tatctcgctg gcgctggttt tgcttagcga tgtatcggca ttagagttag
      661 aaaaatatgc cgataccgcc gtagcgcagg cagcttctcc gctgctcggg catgtgggtt
      721 atgtgatcgt cgtcatcggc gctttactgg cgacggcttc agccatcaac gcgaacctgt
      781 tcgccgtgtt taacatcatg gacaacatgg gcagcgaacg cgaactgccg aagctaatga
      841 ataaatccct gtggcggcag agtacctggg gcaacatcat tgtcgtggtg ttgattatgc
      901 tgatgacggc ggcactaaat ttaggctcac tcgccagcgt tgccagcgcc acctttttga
      961 tttgctatct ggcggtgttt gtggtggcaa tccgcctgcg tcatgatatt cacgcctcgt
     1021 tgccgattct tatcgtaggt acgttggtga tgttgttggt gatcgttggc tttatctaca
     1081 gcctgtggtc ccagggcagc cgtgcgttga tatggattat tggtgcaatc ttactcagcc
     1141 ttattgtggc aatggtcatg aagcgcaata aaaccgtata acacctctct gcgcgcagta
     1201 ctgcctgtac tgcgcctttg tctttaattc aaattacctt tcttattcct ttaataaata
     1261 agtcacaaaa aaatgatata tacgcgataa atctgtgatg ctaatcactt attaccgtgg
     1321 attattctta atgtgtatga tggtaat
//
LOCUS       DAZFKF010000311         1314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFKF010000311 DAZFKF010000000
VERSION     DAZFKF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.316"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(142..1242)
                     /gene="ompC"
                     /locus_tag="ACHKA1_005521"
     CDS             complement(142..1242)
                     /gene="ompC"
                     /locus_tag="ACHKA1_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO5683017.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGKDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT          373 a          289 c          278 g          374 t
ORIGIN      
        1 gatgtccccg gggggtatgg aataatatta attgaggtga atgaccagaa atatcaaaaa
       61 ttgctcattt ttacttaagg agtcatacag cagcacttaa atcaaaaacc agcccagaga
      121 agggctggtc aggagaggat gttagaactg ataggtaatg cccgtagcga cgatgttatc
      181 ggtgcgaata tctacggcac gggtaaagtc gctttcatca atcaggttga ttttataatc
      241 aacataggtc gacatatttt tgttgaagaa atacgtcgca ccaacgtcga tatactcaat
      301 catgtcctgg tcgccgtagc ggcccagatc tttgcctttc gatttcaggt aagcgagtga
      361 tgggcgcaag ccgaagtcga attgatattg cgccaccact tcaaaacttt gtgctttgtt
      421 agagatataa ccatccccat aggtggtcat gtttaatgtt tcagcgtaat tagctgcaaa
      481 gtagatgttg ttggcatcat actttatacc tgtggcccac agttctgcat tcttaccgga
      541 ggcattgaga ggattagcag cggctctttc ctgcgcattt gtccgatcgg atttggtata
      601 taccgcaccg ataccaaagc catcataaac ataagttgag gagataccga aaccgtcacc
      661 gttggcacgc gtgtagtcag caccataaag atgactgttg tcagtacgtt catttttgcc
      721 ttgatattgc gcggcaaagt tcagcccttc aattaagcca aagaaatcgt agttgcgata
      781 ggttgctaca ccggtggtac ggtatgtcat gaagacatct ttctgactcc aggtatcgcc
      841 accaaattct ggcaacatat cagtccatga accaatgtca tagccgacac cgtagttacg
      901 gccatagtcc agagagccaa attcactaat ttttaaacca gcataggcca gacgggtctt
      961 gttgccgcgc gagccgttgg cttcatcgtt atagccttta aaatcatatt cccagtgacc
     1021 aaaaccaatc atttgatcat tgatttgcgt ttctccttta aagccgagac gggcataagt
     1081 tttatcacca tcgtcacgct tatcatcagt aaaataacgt agagcggtaa ccttgccata
     1141 aaggtcgagt ttattaccat ccttgttata gacttcggca gcgtgtgcta cgttcgcagc
     1201 taacaaacca gttacaacca ccgcaactat ttttaatttc atttaacaga atccttttaa
     1261 ttatcgttaa acgtattttc taaacgaatt ttaaacggcg tcatttataa atga
//
LOCUS       DAZFKF010000312         1313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFKF010000312 DAZFKF010000000
VERSION     DAZFKF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.317"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>1313)
                     /locus_tag="ACHKA1_005522"
     CDS             complement(<1..>1313)
                     /locus_tag="ACHKA1_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683018.1"
                     /translation="VFTKLDNNIYSLEVDSFCLYRDYEIARNRLMMRASHLYSEVHEF
                     SNKHFHLNSWVKDHMPSYLNSDGVFSSFHLSELENISPDDLHEEYGNISLFNWVHAYQ
                     CLVELSKEEMSKRFSSTKPIPLQLDRWLIIKSRESWLSFFQRKGIAADAAKKLIDYFT
                     FNSKSHDLNDCPFIPCMDGLCLMPALIANSSVTRSLMSLFGSKKISQASKGRFHEQQF
                     IKQVRDAGIKASPIDAHANYQCDCVILLDDCLIFTELKSNGQPIYYGKYYQQVCNIVG
                     DSSLIHDHNNKFMRSYFQQINRISEHYLNHLDVIIKEFELPSTWQPKGVYKLIVTTTM
                     LGGKYHVDDTYVADKYALSSFFQRIPGVIYQTNENGKMAKNIIDGFECCEGEITIDKF
                     IDYLSSLPSINAVRKNINKLTYSVRFNEKLVHHPYYDSWAFGPYIR"
BASE COUNT          437 a          233 c          206 g          437 t
ORIGIN      
        1 cgaatatatg ggccaaaagc ccaagaatca taataagggt gatggactag tttttcatta
       61 aatcgaacac tataagttaa tttatttata tttttacgta cagcattaat actgggcaaa
      121 gaggaaagat aatctataaa cttatcgata gtgatttccc cttcgcaaca ctcaaaccca
      181 tcaatgatat ttttcgccat ttttccattt tcatttgttt gatatatcac tccaggtatt
      241 cgttgaaaaa aactagataa tgcatatttg tcagctacat atgtatcatc cacatgatat
      301 ttcccaccga gcatagttgt agtaacaata agcttataca cacctttggg ttgccatgtt
      361 gatggtagtt cgaattcttt aatgatcaca tcaagatgat taagataatg ttcagaaatt
      421 cgatttattt gttgaaaata agaacgcata aatttattat tatgatcatg tattaatgag
      481 ctatctccaa cgatgttaca cacttgctga tagtacttac cataatatat tggttgacca
      541 tttgatttta gttctgtaaa aatcaaacaa tcatccagga gaatcacgca atcgcactga
      601 taattagcat gagcatcaat tggagaagct ttaatccccg catcacgaac ttgttttata
      661 aattgttgct catgaaatcg ccctttactt gcttgagaaa tttttttgga accaaataat
      721 gacattagag agcgagtcac tgagctattt gctattaatg caggcattaa gcataaaccg
      781 tccatacagg gaataaatgg gcaatcatta agatcatgtg attttgaatt gaaggtaaaa
      841 taatctatca gtttttttgc tgcatctgct gcaatcccct tacgttgaaa gaatgataac
      901 caactctcac ggctttttat gatcagccag cgatccaact gtaggggtat aggttttgtc
      961 gatgaaaatc ttttgctcat ttcttcttta gataactcga ctaaacattg ataagcgtgc
     1021 acccaattaa ataatgatat attcccatat tcttcatgaa ggtcatcagg agatatattt
     1081 tccaactcgc ttagatgaaa tgagctaaaa acgccatcag aatttaagta agatggcata
     1141 tgatccttta cccaactatt tagatgaaaa tgtttgttac taaattcatg aacttcgcta
     1201 tatagatgtg acgctctcat cattagtcta tttcttgcaa tctcataatc tcggtataaa
     1261 caaaaactat caacctcaag agagtaaatg ttattatcca attttgtaaa tac
//
LOCUS       DAZFKF010000313         1270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFKF010000313 DAZFKF010000000
VERSION     DAZFKF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.318"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..590)
                     /gene="vgrG"
                     /locus_tag="ACHKA1_005523"
     CDS             complement(<1..590)
                     /gene="vgrG"
                     /locus_tag="ACHKA1_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO5683019.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIP"
     gene            complement(773..1081)
                     /locus_tag="ACHKA1_005524"
     CDS             complement(773..1081)
                     /locus_tag="ACHKA1_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683020.1"
                     /translation="MSAEFILSFPDDSWYLRNRNNIVNKILFLSTYRKNDGDMYYLSC
                     DEGEWDYCVRLCIENCSVFIEIFSHPASIEKDLISLFTWIRSQTIISICDNDGEESGW
                     "
     gene            complement(1132..>1270)
                     /locus_tag="ACHKA1_005525"
     CDS             complement(1132..>1270)
                     /locus_tag="ACHKA1_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO5683021.1"
                     /translation="SGIRHIAINILTNDTVFKAGLRRKMRKAAMDRNHLASVLAGCGL
                     S"
BASE COUNT          375 a          301 c          259 g          335 t
ORIGIN      
        1 gggatttcaa aagattcggg cagatggcgg accgtgtcgc acagcaccag gctctggtcg
       61 gtacttttgt aagcatgctc ctcataaaag aagatgcctt cctccgccgc catccgacac
      121 aggaaatcgt aatcagtctc accgtactgg acacaaaact cacgggaagg atgcggttca
      181 ctgaacagcg gactccactc ggtcaccccg ttttcctgca atatcgtgcc gaggatgctt
      241 ttgatgtcct cgttctggaa gatacggaag ttctggcgca gaccggcacg ccacagcggc
      301 gggtgcacct tcatgctgta cagcatctgg tttttgtcgt tctccccgag ttcaaaccag
      361 gtcaccacac ctttcacccg gcgttgaaca tcatcaccct gccataccgt caggtaggcc
      421 attttgtcca gtacctgcgc aaattcaagg gagagaaact gctggctgac cagggagaga
      481 tcgagggaaa agagtgaaga gagtgactgg gtgagatgaa aggaaaccac cgcaaaagcg
      541 tccggtggca ggccgtccac ttccagtgtg aaacgtaatc cggttgacat agcaacctcc
      601 tgtataagaa aaactaacct gcactctgtt gtggaacagc gtgtcaggga ataatattaa
      661 tgagctgatg cccgatatta ccggtaatat atcatgtacg tttctgcgat tttattaata
      721 aaaattcaat tcacatggct catagtattt ctaagagttt acaaatataa tttcaccaac
      781 cagattcttc tccatcatta tcacagattg atattattgt ttgacttcta atccaggtaa
      841 ataaagaaat taaatctttt tcaatagaag caggatgaga aaaaatctca ataaataccg
      901 aacaattttc tatacacagt ctcacacagt agtcccattc cccctcgtca caagataaat
      961 aatacatatc tccatcattt tttctgtatg tagacaaaaa taatatttta ttaacaatgt
     1021 tattcctatt tctcaaatac caagagtcat caggaaatga aaggataaat tcagctgaca
     1081 tttttaatct ccataatttc gacctgaaat agttggaaga ccagggcaag attacgaaag
     1141 cccgcacccc gcaaggactg acgcgaggtg gtttctgtcc atggctgctt ttcgcatctt
     1201 acgtcttaac cctgccttga ataccgtatc attcgtcaaa atattaatag caatgtgccg
     1261 tatccctgaa
//
LOCUS       DAZFKF010000314         1262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFKF010000314 DAZFKF010000000
VERSION     DAZFKF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.319"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            304..1248
                     /locus_tag="ACHKA1_005526"
     CDS             304..1248
                     /locus_tag="ACHKA1_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683022.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDPLKTDEESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNRDEEGHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQCLVIQDDETNLFNLIKIKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPTYFPLKTNEPVTQEDILLLYRFFKMDGDFNILTSDE
                     YMSIMTPLMKCERSIHDHNRYVTGKDTLLLDYPPSGNQIYFHIFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLTDANQIL"
BASE COUNT          393 a          248 c          223 g          398 t
ORIGIN      
        1 tttttataat accaattgat tagagtagcc aatttgttgc tattttacat aacatatttt
       61 tatcaaagtt aattgattgt tgcctttatg agcaccgacc gtaagcccgt tatgttattg
      121 tttcattagt aataatcact taaatgtatc gatgaaacag gtatgactta ccgggagtga
      181 ttgtttctga gtgaacagag gccactttcg cctgataaat gaaaaatgag cgatttttgc
      241 aagttagttt ttctatagcc gagtacgctc agcgcctcgc ttgattacaa tgaggttgct
      301 gctatgagtc ctgataatat cggttcggct gtcaatcgct tctcaggttg gccatctaat
      361 gatcctctaa aaacagatga agaatcaatg acatcacccg tgactgtgca agtaataaag
      421 atgattgccg ccatccttga tccacatcgg atagtcgttg gaattgatac actttataca
      481 acgcagcgcg aaacatttac ctcagagagt caacagctca ttgaatcagc attgcaaaca
      541 cttcatgtga tgaatttaaa taacctggaa acaaatcgcg atgaagaagg tcactatctg
      601 gcttttgaat ataacgttga cacctctgac agaataaccg tttctgctta taatgaaaaa
      661 aaacctggct gctatggcca atgtcttgta atacaggatg acgaaacaaa cttatttaac
      721 ctgataaaaa ttaaaatttt cgaagccagg cataattatg tcgctcaatt gatgagcgat
      781 ccggaattta tgttcaaatt tagttatagc gctcaacaaa atagattaga acctcatctt
      841 ctggttccca cttatttccc tctaaaaaca aacgaaccgg ttacgcaaga ggatatactg
      901 ttactttatc gtttctttaa aatggatggt gattttaata tattaacatc agatgaatac
      961 atgagtatca tgacacctct gatgaagtgt gagcgcagca tccacgacca caacaggtat
     1021 gtgacgggca aagataccct tcttctggac tatcctcctt ctggtaacca gatatatttc
     1081 catatttttc ctgacgaatc agcaacacta gtcctttatt tttcaaacaa tgacattgaa
     1141 tgtttcgtct ttgaaagaga tattccgagc cagtatcgct ttttcaaaat gtttaccaat
     1201 ctggcactgg ttattgatca gttaaccgat gccaatcaaa tactttaatt tttatatatc
     1261 cc
//
LOCUS       DAZFKF010000315         1254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFKF010000315 DAZFKF010000000
VERSION     DAZFKF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.320"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..93
                     /locus_tag="ACHKA1_005527"
     CDS             <1..93
                     /locus_tag="ACHKA1_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO5683023.1"
                     /translation="LGRRATGVELETERFEQTVREVQDLASQNG"
     gene            134..209
                     /locus_tag="ACHKA1_005528"
     tRNA            134..209
                     /locus_tag="ACHKA1_005528"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:167..169,aa:Ile,seq:cat)
     gene            219..295
                     /locus_tag="ACHKA1_005529"
     tRNA            219..295
                     /locus_tag="ACHKA1_005529"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:253..255,aa:Arg,seq:tcg)
     gene            309..385
                     /locus_tag="ACHKA1_005530"
     tRNA            309..385
                     /locus_tag="ACHKA1_005530"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:343..345,aa:Arg,seq:tct)
     gene            475..699
                     /locus_tag="ACHKA1_005531"
     CDS             475..699
                     /locus_tag="ACHKA1_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683024.1"
                     /translation="MKKLMVTIGPFETEVSFRVVQGESVLVEDVFHGKSTGPYVKEYL
                     IDATDENIEVVYDSVNHPDLIIKAKLKPLY"
     gene            752..961
                     /locus_tag="ACHKA1_005532"
     CDS             752..961
                     /locus_tag="ACHKA1_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683025.1"
                     /translation="MFVSEVLMEDKDNKGWVKGWAVVRSSPWHLVGVFATEEDAETEA
                     RKMGDKYEVHYGSHRTGSDDFVWGE"
     gene            1100..>1254
                     /locus_tag="ACHKA1_005533"
     CDS             1100..>1254
                     /locus_tag="ACHKA1_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HFO5683026.1"
                     /translation="MGRSLPGVGSPTQRNVTMFGIFKKKTRRAATEIKKFEKRDLAQA
                     VINAAYLV"
BASE COUNT          326 a          261 c          341 g          326 t
ORIGIN      
        1 ctggggcgtc gtgcaactgg cgttgagctg gagactgaac gttttgagca gacggtcagg
       61 gaagttcagg atttagccag tcagaacgga tgatattgca ggattagtta cgtaccgtta
      121 ttatcctgca cccggccctt tagctcagtg gtgagagcga gcgactcata atcgccaggt
      181 cgctggttca aatccagcaa gggccaccat atcacatacc gccattagct catcgggata
      241 gagcgtcagc cttcgaagct ggttgcgcgg ggttcgagtc ctcgatggcg gtccattatc
      301 ggtattcagc gttgttagct cagccggaca gagcaattgc cttctaagca atcggtcact
      361 ggttcgaatc cagtacaacg cgccatactt atttttctgg ctcgcttttg cgggcctttt
      421 ttgtatctgc gttacaccat taactaataa atcgagtgct tatcaggagg ctatgtgaaa
      481 aaactgatgg tgacgattgg tccgttcgaa acagaagtta gttttcgtgt tgttcagggg
      541 gagagtgtac ttgttgaaga tgtatttcat ggaaaatcaa caggtcctta tgtaaaagaa
      601 tatcttatcg acgccacgga tgaaaatatt gaggtggtgt acgattccgt caatcaccct
      661 gatttgatca ttaaggcaaa attgaagcca ctttattgat ctgaccggga gcaatcataa
      721 aatatctctg ggtacccaca aggagataaa tatgtttgtt tctgaagtgt taatggaaga
      781 taaggacaac aaaggatggg ttaaaggttg ggctgtggta agaagctcgc cctggcatct
      841 tgttggggtt tttgcgacag aggaagacgc agaaacggaa gcaagaaaga tgggagataa
      901 gtacgaggtt cactatggct cgcatcgaac aggaagtgat gattttgtct ggggggagta
      961 acagtcgttt aaccccagaa taatcccgta actgaggtcg ctattggcgg ccttttttgt
     1021 atccgcgcca cgcccggcgc atatcaacca cagagccttt cgggggtgag cttacggagt
     1081 ggtcagtgtg actttctctg tgggcagatc gctcccgggc gttggctcac ccacccaaag
     1141 gaacgtcacg atgtttggta ttttcaaaaa gaaaacccgc agagcagcaa cggaaattaa
     1201 aaagtttgag aaacgcgatc tggcacaggc ggttattaat gctgcctacc tggt
//
LOCUS       DAZFKF010000316         1242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFKF010000316 DAZFKF010000000
VERSION     DAZFKF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.321"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            216..323
                     /gene="yniD"
                     /locus_tag="ACHKA1_005534"
     CDS             216..323
                     /gene="yniD"
                     /locus_tag="ACHKA1_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO5683027.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(376..1134)
                     /locus_tag="ACHKA1_005535"
     CDS             complement(376..1134)
                     /locus_tag="ACHKA1_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO5683028.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          353 a          293 c          284 g          312 t
ORIGIN      
        1 gcatgagttt tagtgctgat gaattaaaga cgtatttata accagctatt tacgatattt
       61 atatatcgat acataatttt attttttcat aaaacgcaga aacagtgatc aggtgtacaa
      121 cgccacccga cggctgtaat aacaaaaaat cccttgatgc ctgtcccttt tgttacactc
      181 cgttatcacg cacaagagat atgcaggaca ctggtatgcc gactaaacgc tttgataaaa
      241 aacactggaa gatggtggtg gtgctactgg caatctgtgg cgctatgttg ttgctacgtt
      301 gggcagcaat gatttggggc tgagcagtga caaacgaaag cagccagacc gcataacacg
      361 atccggcaga taattttaca tgctgtagcc cagcgataag gttgtccgac gatcggtatg
      421 ttccggtgcg gactctggag gttcagagtt ccatgtgacg ttgtaagcca ccttcagtcc
      481 aaaatgttca ttaatagcaa catttaatgc actttcggag ttcagcgttg tgtcttccgc
      541 gccaaagacc gaaacaccct gcgtaaattt agcgttgtca gtcaactgcc atgcataagc
      601 cccggaggca tagcccagcg gctgagtttc actggcattg tcggtatatt tgtcgtaacg
      661 cacacctgga ccgaattcaa agcggaaact gtgtaccggg ccattcaaaa actgacgacc
      721 ataacccgcg gtcaacacat cgcgctctcg atagccgtta taacggtctg tcagccagct
      781 tgcctgacca aataaatagt cataatcagt taaattaaaa cggctacgcc cgcccgccgc
      841 atatttttct gaagaacgct catcattaga agaagtatta ctggcgttcc cccacagcga
      901 ccaggcagtg gtttgtccat accaggtcat ggtggtgtca gccgtaagtg aggagctttt
      961 cgtattgcct gattgtgcaa gatatcctgc gttcagatta ccttcgaaag gttttttagc
     1021 gctggcagga tcgtccatga cagtaaaaac ggaatcatcg gcagctgcat tcagtgacgc
     1081 aaacatgccc cccgccaaca taacgatggc aggaactgtc ttcaaaagct tcatttatca
     1141 agagtccgta caacaaaaaa agagaccatc gcggtcccgg aaactttctt aaggatcaaa
     1201 gattagcgtc cctggaaagg taacgaatta taaaaaggcg cg
//
LOCUS       DAZFKF010000317         1197 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFKF010000317 DAZFKF010000000
VERSION     DAZFKF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.322"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..452)
                     /locus_tag="ACHKA1_005536"
     CDS             complement(<1..452)
                     /locus_tag="ACHKA1_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683029.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVA"
     gene            910..>1197
                     /locus_tag="ACHKA1_005537"
     CDS             910..>1197
                     /locus_tag="ACHKA1_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683030.1"
                     /translation="MKIYWTRKSIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILFRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYY"
BASE COUNT          378 a          270 c          223 g          326 t
ORIGIN      
        1 gccacgtatt gccctgatga accaggccgg gcaacccgaa aacatctttc cacccggcat
       61 tgtccgccag cacttatcac cgaccaggtc tgttgcagta aatccaccag ttgcgcacat
      121 tcttcatcag ccatgacgcc gactcgcgga tggcaaatac tcgtctggct gagcagcaac
      181 agacatcgta tctgtgctcc ttcatgttgt tcccgcaacc agcgtaatac caccggcgct
      241 gatgggctga ccgtgtgatt atgctccgta atacgttcgt gtacccgtcg gtgaaaggaa
      301 ctacaaaatg tctgaatctg ttgaccatag attccttctg ccgaatagag cgtgaagtga
      361 aatgccaaca gatttggcca gtggtccacc gcctgctgaa caaaacgccg gatttccccc
      421 ggctctgaaa ttaaggcttc ggttatttgc acgaaatgtt ccttttcaaa ccctgttgaa
      481 ttttgtcaag ccggtgcaga cacagtaaag cgcgcatcaa cacctgaaag gtacgataca
      541 aacacaagtt acaaacaata agaacggaag gacaattaat cagtgaggaa taataactaa
      601 attaaccaaa agaatgttac tgacaatacc accaatactt aaatagcaat atattaatta
      661 atgtaagaac agatgcccgg taagacatat acacaataca tccacaaaca cactcttttc
      721 attaaatcaa tcacctgatt aaaccatgaa agcgtgatgt aagtcaaaat tactgacaca
      781 atcaattgat aatgattatc tctatcatta tcgtgaaatt cgagaatgaa aggagaagta
      841 gtcattataa gccttttata aatcgtcatc atcatagtaa agtatgaaca cttttatatg
      901 agttgccata tgaaaatata ctggacaaga aaaagcattc ctgaactttc agcacttcca
      961 ccttcattaa gaaagaaaaa ttttactgac gcatataatg ctgcatcttc ccatatcgaa
     1021 tactggatag gtgctggcgt ctcatttatc tcgatgatga ttttattcag agtatatgac
     1081 tttcttttac ccgcccaaga tactttcccg ggggatatca tcagaagttt atgtgttgtg
     1141 tgtccatcca tattaatctg gtttcagttt tcggtatatg tcatgaggaa atattac
//
LOCUS       DAZFKF010000318         1195 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFKF010000318 DAZFKF010000000
VERSION     DAZFKF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.323"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            60..347
                     /locus_tag="ACHKA1_005538"
                     /pseudo
     CDS             60..347
                     /locus_tag="ACHKA1_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            791..>1195
                     /gene="espR1"
                     /locus_tag="ACHKA1_005539"
     CDS             791..>1195
                     /gene="espR1"
                     /locus_tag="ACHKA1_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR1"
                     /protein_id="HFO5683031.1"
                     /translation="MKFPSIFNKIKPQSIQQHPEKNQLNWMLELNKWKEERILTGEIH
                     RPECRNEAAKRINCAFLSKQNDIDLSGLNLTTQPPGLQNFTSINLDNNQLTHFDATNY
                     DRLVKLSLNSNALESINFPQGRNVSITHISMNN"
BASE COUNT          444 a          245 c          178 g          328 t
ORIGIN      
        1 tagcatcgct ggatgtctcc cggcaagaat ctctgattga gttaagtgcc catcacaata
       61 tgatcactga ccttatatta cacaatcatc ccaggatgaa aacaatcact ttaaacgaca
      121 accatattgc acatttaaac accaaaacca ctacaaaact tgaatattta aacttaagca
      181 ataacaattt attgccaacc aatgacattg atcaactaat atcatcaaaa catctttggc
      241 atgtattagt taacggcatc aacaatgatt cacttgcaca aatgcagtac tggactgcag
      301 taagaaatat aattgatgac actaatgaag tgaccattga tttatcttat aacctggcaa
      361 tcacaaatat cgataccagt gatgaacatc ttgcagaaat aagcgataat tcagaaggta
      421 attatataaa agacaatgac tcaatgtcta ttcgttatag atcaaaatat tattccagag
      481 attccgcctt aatagaagaa gaaacaatat tttctgacgc agaactaaaa gctattctgc
      541 ctatgcgtcg catgtacggg gttggtgact ataagtcaaa ttcctcttct ctatcctcac
      601 tctcggggct caaggaccca acgggcacac ccgtctgtta ttatattcat aatgagagta
      661 aaccttcctt aggttatggt ccaacatgca ataattggtt aagccaatcc tttacaacag
      721 agttataaaa cacatcctga tttatcaagg gtgataaatc agaaaaacat tgtattacta
      781 cggagcagtt atgaaattcc cttcaatatt taacaaaata aaaccacaat ccatacagca
      841 acatccagaa aaaaatcaac ttaactggat gctcgaatta aataaatgga aagaagaacg
      901 tatacttaca ggtgaaatcc atcgtccgga atgtcgaaac gaagccgcta aaaggataaa
      961 ctgtgctttt ttgtcgaaac agaatgacat tgatttatca ggacttaatt taactactca
     1021 accaccaggg ctgcaaaact tcacctctat caatcttgat aataaccaac tcacacattt
     1081 tgatgcaacc aactacgata gactcgtaaa acttagtctg aatagtaacg ctcttgagtc
     1141 aataaatttt cctcaaggca gaaatgtaag cattacacat atatctatga ataat
//
LOCUS       DAZFKF010000319         1176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFKF010000319 DAZFKF010000000
VERSION     DAZFKF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.324"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            222..>1176
                     /gene="espX2"
                     /locus_tag="ACHKA1_005540"
     CDS             222..>1176
                     /gene="espX2"
                     /locus_tag="ACHKA1_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFO5683032.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISINMNDAIPRDEKGITVR
                     IDNINGKKNNSNSSDVFIPYVNTFPDLKNKILRMKIELTEGSGFSKSLSDSQIEMHIL
                     RNVNSLNVGEKLNDDNLAYHSIFTNEFSVIIPPSYYDATSVVNANNGVREKLFESDSK
                     VKDIVDDMSNHDVESEKDIFVIGGMIEKLDSLVDESFNENTDNIQTVKDLLTQLTDGM
                     ELFALRDIVAFPSSIIAKLLKSTLNSDHELVMRALDTYLCYF"
BASE COUNT          397 a          190 c          228 g          361 t
ORIGIN      
        1 acaatgaagt aatttaaatt aactatcaaa aatcgctcat ttctgcttat tgatagaaag
       61 attgatcgcc agtctgttta gagcggtgca gcagcaaact ttcataacca ggttaatcct
      121 gagtttgcag tacgaaaagt taaaaatgag cgatttctgt aagattcccc attttgccag
      181 gcttatgatt cgcagatcat tcaagtgcaa aggagcgatg tatggattgt tcaaaatgca
      241 atggttatgc gacgatgtta ttaaatatgg tgcagggttc tgacccggtt aatttactgg
      301 aattacatgg tttccttgaa catttcgcat attatgtttc atttggcaaa tttaatgctg
      361 gccaccagcg ctataatgca tttaaaaaat tcgtcagtga gatttcggag atcagcgcaa
      421 acgatatcaa tatgacgatt aaaaccgggc aatccagaca tgaaaatgtt atctctataa
      481 atatgaatga tgcaattcct cgtgatgaga aagggatcac cgtcagaata gataatatta
      541 atggaaagaa aaataactct aattcatcag atgtttttat tccttatgta aatacatttc
      601 ccgacctcaa aaataaaata ctcaggatga aaattgaact gactgaaggt agtggttttt
      661 ctaaaagtct ttctgacagt cagattgaga tgcatattct gaggaatgtt aattcgctaa
      721 acgttggcga gaaattgaat gatgataatc ttgcatatca ttctatattc actaacgaat
      781 tctctgttat tattccgcca tcctattatg atgcgacttc tgtagtaaac gccaataatg
      841 gtgtgagaga aaaactattt gaaagtgata gtaaagttaa agatatcgtc gatgatatgt
      901 ctaatcatga tgtggagagc gaaaaagata tatttgttat cggaggaatg atagaaaaac
      961 tggattctct ggtagacgaa tctttcaatg aaaatactga taacatacaa acagtaaaag
     1021 atttgttaac gcaattaact gatggcatgg aactcttcgc gctgcgggac atagtggcat
     1081 tcccatcgtc cataatagca aaattattaa aatccacact taatagcgat catgaacttg
     1141 ttatgcgcgc attggatacg tatctgtgct acttca
//
LOCUS       DAZFKF010000320         1143 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFKF010000320 DAZFKF010000000
VERSION     DAZFKF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.325"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(126..>1143)
                     /locus_tag="ACHKA1_005541"
     CDS             complement(126..>1143)
                     /locus_tag="ACHKA1_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO5683033.1"
                     /translation="AEGWEDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPT
                     KFHECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQ
                     IKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQG
                     RLNKAFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLK
                     KLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVV
                     MNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAG
                     SGLS"
BASE COUNT          283 a          296 c          215 g          349 t
ORIGIN      
        1 accacactgc caccgtcttc gccgccggac tgctgaagcg catccgccag gctgcggcgc
       61 tgcgttttcg ccagctcacc ggtggcggtt tcaaccctga tgagtggcgt gaagccaggg
      121 caagattacg aaagcccgct ccccgcaagg actgacgcca gatagtttct gtccatggct
      181 gcttttcgca tcttacgtct taaccctgcc ttgaatacct tatcattcgt caaaatatta
      241 atagcaatgt gccgtatccc tgaaaataat tctgctgcat ttcctcttct tattttgcag
      301 tcgtcttcat tcattaccac gtccagacgc cagtgcagct tattctccac gtgccagtga
      361 tttcggatcg ctgtggcgaa tttctcagcg gttaaatcag cagaactgat ataatatctg
      421 accgtcattt ctggctcttt cttttgctct gctattatgg accgaaagga gactgccacg
      481 cataatttct tcagcccttt ccattcaaac gtgaaatcaa taagttcatc agggacatcg
      541 caaacaatat gaagacggat ttcttctctg ccgtgactct tttcactaat tgcgtaactg
      601 tcatgctctg gattatttaa ttctttcagc ggaaattttt cctcaaaggc tttattaagc
      661 cgcccctggt taccttttac cgcgaataaa taatcaccgc cctgtttttg tatcttctct
      721 gcgatatctt tctggcaacc catcgcatca gttgtgataa ttttcccttt aatatccagc
      781 atgttaagaa gttcagggat agctgtaatc tcattagatt tctcatccgt cttgatctgt
      841 ccgatgacca gactgtgcat tgttgagaac gcactaatga catgaatcgc tcccctgcgg
      901 cgactcttgt cataagaatg ccggagcgtt tttccatcaa ttgcaatgac gtctttatca
      961 tctgaagaat gacaatcacg catccagtta ataaagcact catgaaattt tgtagggctg
     1021 atacaggata caactctggc aatggtatcg tgaacaggaa taccattttc aaaatcacca
     1081 tattgcttca aaaaatcgag atgtgtttcc ccaaaatcct ctatatcttc ccaaccttct
     1141 gca
//
LOCUS       DAZFKF010000321         1125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFKF010000321 DAZFKF010000000
VERSION     DAZFKF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.326"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..842)
                     /gene="mchF"
                     /locus_tag="ACHKA1_005542"
     CDS             complement(<1..842)
                     /gene="mchF"
                     /locus_tag="ACHKA1_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HFO5683034.1"
                     /translation="MTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLAMICGHF
                     GKNIDLISLRRKFNLSARGANLAGINGIAEQLGMVTRALSLELDELGALKMPCILHWD
                     FSHFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGIALEVWPGSEFLAETQQTRI
                     SLRSLINSIYGIKRTLAKIFCLSVVIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISA
                     GLMFFILLRAAVSMLRAWSSLVMSTLINIQWQSGLFNHLLRLPLAFFERRKLGDIQSR
                     FGSL"
     gene            complement(835..>1125)
                     /locus_tag="ACHKA1_005543"
     CDS             complement(835..>1125)
                     /locus_tag="ACHKA1_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V secretion protein CvaA"
                     /protein_id="HFO5683035.1"
                     /translation="GQFSATVKTISRTPASTQEMLTYKGAPQNTPGDSVPWYKVIAMP
                     EKQIIRYDEKYLPLENGMKAESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
BASE COUNT          298 a          294 c          253 g          280 t
ORIGIN      
        1 tcaagggagc caaaacgcga ctggatatca cctaatttac ggcgttcaaa aaacgccagc
       61 ggcagtctga gaagatggtt aaacagaccc gactgccact gtatattgat gagcgtgctc
      121 ataaccagtg aggaccatgc acgcagcata ctcaccgcgg ccctgagcaa tataaagaac
      181 atcaggccag cagaaataag cgtcagcagc cctctgtccc ccgccggaat cgcatgatcc
      241 ataaccagct gagttcccac cggcattacc agattgattg cttcaattac aactgacaga
      301 cagaaaattt tcgccagtgt tcttttaata ccgtaaatac tgttaatcag tgaacggaga
      361 cttatgcggg tctgctgggt ttccgccagg aattcacttc cgggccagac ctcaagtgca
      421 atgcccgtaa aataccggct catttcctcc cgaccgagat atcttctgcc tctggccgga
      481 tcatgcagta cataacggtt acgctttacg ctgaccagca cgacgaagtg actgaaatcc
      541 cagtggagaa tacacggcat tttgagggca ccaagttcat ccagctccag tgaaagagcc
      601 cgggtgacca tccccagctg ctccgctatt ccattgattc ctgcaaggtt tgctccacgg
      661 gccgagagat taaacttccg gcgaagagat atcaggtcaa tattcttacc aaaatgaccg
      721 catatcattg ccaggcaggc cagtccacat tcagcggtct ccgtctgatg aataaccgga
      781 acacgacgtc gccagcgcat atcaagctga tttataattt gtctgaaatt cccgttagtc
      841 attgagcggt cctgttgcac tgtgtttcat gtcatagaaa ggagaaagca tccactggta
      901 aatacgcctt ttttccagaa atagtgtact ttcggctttc attccatttt ccagagggag
      961 gtatttttcg tcatatctga taatctgctt ttcaggcatc gcaatgactt tataccaggg
     1021 aacagagtcg cccggcgtat tctgtggtgc tcccttatag gtcaacattt cctgtgttga
     1081 cgcaggagtc ctggatatag ttttaaccgt agcagagaac tgccc
//
LOCUS       DAZFKF010000322         1116 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFKF010000322 DAZFKF010000000
VERSION     DAZFKF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.327"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..209
                     /locus_tag="ACHKA1_005544"
     CDS             <1..209
                     /locus_tag="ACHKA1_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin"
                     /protein_id="HFO5683036.1"
                     /translation="WRALGGKLKVTQLSSTGYLGQFDFCAIARMGNAEDSHYCQVVES
                     PSGSRKWYKYEHKTGCIASCVTLN"
     gene            604..864
                     /locus_tag="ACHKA1_005545"
     CDS             604..864
                     /locus_tag="ACHKA1_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFO5683037.1"
                     /translation="MIAKLLPLQLLRGVGLEQRQHVHLARKSLVEAVNADQIPDLYGS
                     HVPCLQATMHGLPVATRPKIKMVQLQFTPFASDDTYHWQTTM"
     gene            complement(843..>1116)
                     /locus_tag="ACHKA1_005546"
     CDS             complement(843..>1116)
                     /locus_tag="ACHKA1_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shufflon protein D'"
                     /protein_id="HFO5683038.1"
                     /translation="RASGSTVLTGKIANGQQIPLPSGFSASQCTWSVSNAENPHGWKP
                     NYFAGSVATYDANRIVKCGFYDEYNFYGGTHRTDLSGKCSYIVVCQ"
BASE COUNT          297 a          271 c          266 g          282 t
ORIGIN      
        1 tgtggagggc attaggtgga aagctcaagg ttactcaact ttccagcact ggttacttgg
       61 ggcaatttga cttttgtgcc attgccagaa tgggaaatgc tgaggattct cattactgcc
      121 aggtagtcga gagtccatct ggttcacgaa aatggtacaa atacgagcac aagacaggtt
      181 gtattgcgtc ctgtgtaact ctcaattaag tttggtatcc aaatactgaa aatacccctg
      241 agccacaaga gtagttttgg gccggataag aggtgatctg ataagttgcg ccagcaggga
      301 cagcaaaaga gatgaatgct gtcttcccat aagatgggtt gttgctcgca tttgtgctaa
      361 tcaatgcccc tgcaacgtat ccctgcaatc tggatgtgtt agcgcaatct cccccggcgg
      421 aaccaccatt ccctccagag gcgtaaacaa acagcgtacc acttcctgta ttgcgaccag
      481 taaatgatcc tctgtgtgag ccaagattac tgtaactacc gttagaggaa ccggaagttc
      541 tccacacacc ggattggcac tggcctccgt gccaatccgg tacgtggaaa aaaattggcg
      601 caggtgatag ccaaattgtt accgcttcag ctactgcgtg gcgttggcct ggagcaacgg
      661 caacatgtcc atctggcaag aaagtcattg gtggaggcgg tcaatgcaga tcaaataccg
      721 gatttatatg gctcacacgt tccatgcctt caggcaacaa tgcatggact gccagttgcg
      781 acacgaccga agatcaaaat ggttcaatta cagtttacgc catttgccag tgacgatact
      841 tatcactggc aaaccacgat gtaactacat ttccccgaca ggtcagttcg gtgagtaccg
      901 ccataaaagt tgtattcgtc atagaagccg catttcacaa ttcgattggc atcataggtt
      961 gcaactgacc ccgcaaaata attcggcttc cacccgtgcg gattttctgc attactgaca
     1021 ctccaggtac actggctggc agaaaaacca gatggcaatg ggatctgttg cccatttgct
     1081 atttttcctg tcagaacagt gctacctgat gccctt
//
LOCUS       DAZFKF010000323         1112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFKF010000323 DAZFKF010000000
VERSION     DAZFKF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.328"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..549
                     /locus_tag="ACHKA1_005547"
     CDS             <1..549
                     /locus_tag="ACHKA1_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO5683039.1"
                     /translation="PLGLDLLSLPSPRQKPVSQSCTIDPINQQPVGRFIGDSKGNIMV
                     EPVGGRTIPYPPSNPGSADTHTLYQNNSNAYRLNPIGHANNSTPHAHAHLPGTGSGMK
                     GQGPSLDVNGRVVPWNSSAAHIGIRSLGTLSFFSSVLHMFTLRSCREGTFGQCYCAMY
                     VEMSTGSNQDEADIMCGMPEYF"
     gene            653..964
                     /locus_tag="ACHKA1_005548"
     CDS             653..964
                     /locus_tag="ACHKA1_005548"
                     /inference="COORDINATES: protein motif:HMM:NF040040.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6258 family protein"
                     /protein_id="HFO5683040.1"
                     /translation="MSRIAYGTTEWNFYDNEDLEDGYLVFSDVSFFNITPNGSIPDDY
                     IISIITDNVNVEYFESTIVAVGQIPNTNSGDIDNIGECQIFIRYKHSWIENKFKEKII
                     E"
BASE COUNT          366 a          179 c          230 g          337 t
ORIGIN      
        1 cctttgggat tagatctatt atcattacct tctcccagac aaaaacctgt aagtcaatcc
       61 tgtactattg atcctataaa tcaacaacct gttggtaggt ttataggtga ttctaaaggc
      121 aatattatgg ttgaaccggt aggtggcaga acaatcccgt atccccccag taatccagga
      181 agtgcagata ctcacactct ttatcagaat aattcaaatg cttataggct taatccaata
      241 gggcatgcaa acaattcaac accacatgca catgcacatt taccaggaac aggtagcggt
      301 atgaaaggcc aaggtccatc ccttgatgtt aatggtcgcg ttgtaccatg gaatagtagt
      361 gccgcacata tcggtattag atcactcggt acactaagct tttttagttc tgttttacat
      421 atgtttacac ttagatcatg tagagaggga acgtttggac aatgttattg tgctatgtat
      481 gttgaaatgt caacgggaag caatcaagat gaggctgata taatgtgcgg aatgcctgag
      541 tatttttaga ataggggggt aatatgtttg atagaatata tcttggtgat cgagcaataa
      601 aaaaattgaa tttgatttat ggagtaaaca aattagaatt caggttaatt taatctcaag
      661 gatagcctat ggtacaacag aatggaattt ttatgataat gaagacttag aagatggtta
      721 tttggtattt tcagatgtta gtttcttcaa tataactcct aatggctcta ttccagatga
      781 ttatataatt tcaataatta cagataatgt caatgttgaa tattttgaat caactatagt
      841 tgcagttgga caaattccaa atacaaattc tggagacatt gataatattg gtgagtgtca
      901 aattttcatc aggtataaac acagctggat tgagaataaa ttcaaagaaa aaataattga
      961 ataaccatgt tatgtataga cattttagat ggaacgaacc agaggaaggc atgcctccga
     1021 cttgggttat atgggcacgg agttttagtg tagtgaactc aacgacgcaa accgaataac
     1081 ctgccgctgg cgcttatcag cacggaaggg gc
//
LOCUS       DAZFKF010000324         1109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFKF010000324 DAZFKF010000000
VERSION     DAZFKF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.329"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1109
                     /gene="eilA"
                     /locus_tag="ACHKA1_005549"
     CDS             <1..>1109
                     /gene="eilA"
                     /locus_tag="ACHKA1_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /protein_id="HFO5683041.1"
                     /translation="GVIILLLNADGEIVSKEEIIDKVWSASVASDESLTRCIYALRKL
                     LHENKQCKYIETVYGRGYRFTVPIVIVTDNEPVKSTTTLAVFPFRTEGSINVLKLHYE
                     LVQGLSKYAFCGLDILPASVTNEASDFSTIHQFINKTGPEYYIMGQVVHYGKNWRLFI
                     ELIHARTHKLIDHQSIDFNPDNPLSVLLSQVINILIEKIPNINFKSINTQQMPSLDSA
                     VMYMNGRMEMYRYTPESLKRALVIFRDCVSTQPQNTLPYCCLAECHISLALLGISEQK
                     QAITTAVTSIEKALEINPSNSQALGLLGLISGLKDEHSVSNVLFKQAHLLKPNSPDVY
                     YYQSLLYFLNGDLARAFNLIEKSIALEPNKMGISI"
BASE COUNT          347 a          212 c          203 g          347 t
ORIGIN      
        1 ttggcgttat cattctatta cttaatgctg acggtgaaat tgttagtaaa gaagagatta
       61 tcgataaggt ctggagcgcg agtgttgcca gtgatgaatc tttaacacgg tgtatttatg
      121 cattgcgtaa acttttgcat gagaataaac agtgtaagta tatcgaaacg gtctatggtc
      181 gtggataccg ttttactgtc cccattgtga ttgtaaccga taatgaaccg gtaaagtcga
      241 ccaccactct tgccgttttt ccctttcgta ctgaaggttc gatcaatgtt ctaaaactcc
      301 attacgaact ggtccagggc ctttcaaaat atgctttttg tggtttagat attttacccg
      361 cttcggtaac gaatgaagcc agtgatttct ccactatcca tcagtttatc aataaaacag
      421 gcccagaata ttatattatg ggtcaggtcg ttcactatgg taaaaactgg cgtttattta
      481 ttgaattaat ccatgccaga acgcataaat tgattgatca tcaaagtatt gatttcaacc
      541 ccgacaatcc tctttccgtg ctattatcac aagtaattaa tattcttatt gaaaaaatac
      601 ccaacattaa ttttaaatcc attaatacac aacaaatgcc atctctggat tctgctgtta
      661 tgtatatgaa tggcagaatg gaaatgtacc gttatacacc agaaagtttg aaacgagcat
      721 tagtaatatt tagagattgt gtgagtacgc aaccgcaaaa taccttgccc tactgttgcc
      781 tggcggaatg ccatatatca ttagcactcc ttggaatcag tgaacaaaaa caagcaataa
      841 ctacggcagt aacctccatt gaaaaggctc tggaaattaa cccttcaaat tctcaggcat
      901 tgggtttact gggtttaatc agtgggctaa aagatgaaca ttcagtgtct aatgtgctat
      961 ttaaacaagc acatttactc aaacctaatt cgccagacgt ttattattat cagtcgctgc
     1021 tatatttttt gaacggcgac cttgcaagag catttaattt aatagaaaaa agcattgctc
     1081 ttgaacccaa taaaatgggc ataagcatt
//
LOCUS       DAZFKF010000325         1094 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFKF010000325 DAZFKF010000000
VERSION     DAZFKF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.330"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1082
                     /locus_tag="ACHKA1_005550"
     CDS             <1..1082
                     /locus_tag="ACHKA1_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO5683042.1"
                     /translation="AATPKAVKAAMDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYG
                     RDIHTGAGEWKTREFVAWLKEKGAFDQPYWMMKASLPASMNKVITDVGPGKLNLGGCV
                     IEVMGTYEAAIVRVTIGEYGATGFINGTVCTCTVYGDTRYFHWRVDYSTKNKPDTVSQ
                     RDASTTQKGVVQLSSDTGSNDETKAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPK
                     GEKGDPGAQGPKGETGATGPAGPQGPKGEKGDPGPRGERGETGPMGPPGASGDDGKSR
                     VMGFRLGNPVTYPLQEGENGWSVDLADGSVITGLSGNTQGGVSRVTTIYYRPLQMTFD
                     GSHWRTVGVGEYVSSGASGREYFPL"
BASE COUNT          287 a          235 c          375 g          197 t
ORIGIN      
        1 aggcagccac cccgaaagcg gtgaaagcgg caatggacaa agcggacgga tgcctggaga
       61 aagcgaaaaa cggtgacgat atcccggata aggtgaagtt tctgaacacc gtgggagcag
      121 ccagagtata cgggcgggac attcatacgg gggccggtga atggaaaaca cgcgagtttg
      181 ttgcctggct gaaagaaaaa ggggcatttg accagcctta ctggatgatg aaggcatcac
      241 tgcctgcgtc aatgaataaa gtcatcacgg atgtcggacc gggaaaactc aatctgggtg
      301 gctgtgtcat tgaggtgatg ggaacgtatg aggctgccat agtccgggtc accattggcg
      361 aatacggtgc aacggggttt attaatggca cggtctgtac ctgtacggtt tacggcgaca
      421 cacggtattt ccactggcgg gtggattaca gcacaaaaaa caaaccggat acggtcagcc
      481 agcgggatgc cagcacgacg cagaaaggtg tggtgcagtt aagcagtgat actggcagta
      541 atgacgaaac aaaggcagcc acgccgaagg ccgtgaaggc ggcaatggat gtggcaaatg
      601 aagcgaaaac aaaggcagaa gaggctgcag caggaggtgg tgttcccggt ccgaaaggag
      661 agaaaggcga ccccggagca cagggtccga aaggtgaaac gggagcaaca ggccccgctg
      721 gtccgcaggg accgaaaggg gagaaaggcg accccggtcc cagaggtgag cgtggagaaa
      781 ccgggccgat ggggccgccg ggggcatcag gagatgacgg gaagagtcgt gtcatgggct
      841 tcaggctggg gaatccggta acgtatccgt tgcaggaggg tgaaaatggc tggtcagttg
      901 atctggcaga tggttcagtc attacagggc tttcggggaa cactcagggc ggcgtcagcc
      961 gtgttacaac gatttactac aggccgttgc agatgacgtt tgatggctct cactggcgca
     1021 ccgttggtgt tggtgagtat gtgtcgtcag gagcttcggg gcgtgagtat tttccattat
     1081 aaagcgcatc atct
//
LOCUS       DAZFKF010000326         1041 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFKF010000326 DAZFKF010000000
VERSION     DAZFKF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.331"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..159
                     /gene="yacA"
                     /locus_tag="ACHKA1_005551"
     CDS             <1..159
                     /gene="yacA"
                     /locus_tag="ACHKA1_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFO5683043.1"
                     /translation="RQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDK
                     MSRKVAGK"
     gene            159..437
                     /gene="yacB"
                     /locus_tag="ACHKA1_005552"
     CDS             159..437
                     /gene="yacB"
                     /locus_tag="ACHKA1_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFO5683044.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAG
                     QPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            483..>1041
                     /locus_tag="ACHKA1_005553"
     CDS             483..>1041
                     /locus_tag="ACHKA1_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO5683045.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKRPLTSKQLLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGGRPVIIFNA"
BASE COUNT          252 a          256 c          314 g          219 t
ORIGIN      
        1 cgccagaccg ttgaacggca gcataatacc tggttccgcg accaggttgc ggcaggacgt
       61 cagcaactcg agcacggcga tgtgcttccc catgacatgg tcgaatcctc tgctgcagca
      121 tggcgggata aaatgagcag gaaggtcgcc ggtaaatgat ggagatattc tggactatgc
      181 tggccagcca ggacaggaag cgcattcgtg agtacattgc ggagcagaac ctgatggccg
      241 ccattgaact ggacgaacgg attggttatt cagccagcag cctggccggg cagccgtata
      301 aaggccgcaa cggtcgtgtg gaaggtaccc gggaactggt tattcacccg cattttgttc
      361 tggtttatga ggtcgacagc cagtggggaa aagtgtatat cctgcgtgta ttgcatactg
      421 cacagaagtg gccatagaga ggctgtccgg atataaaaac gtctgacgga gatgcaagaa
      481 ccatgaataa tgatgagctg gccaccagac gtgcgcaggc gattgctgaa gacagatgtt
      541 tctcgaaagg acgtctgcgc gatgaattca gaatgaagcc tgcccccggt gctgagccgg
      601 ttaagtggta taaaaacact tatggtggca ggttcgctgt ataccgaatt gcagattgtg
      661 ttccgatgcg tgagaaacgc ccactgacga gtaaacaact tctggctggc cagcgacttt
      721 ccgttttatc cagacttaac agcaccagtg gccggatggc acggcaggca tatgactggc
      781 tgagtctggc accgctcttt ctggatactg aaacgacagg actggataat accgcggaag
      841 ccctggaaat cggccttact gatgcggccg gtcaggtggt atttgaaacc agactgaagc
      901 ccacggtcgc catcggggcg caggccgccg ctgtgcatgg tatcagtgag caggctctct
      961 gcggcgcacc atcatggaca gatgtggcgc ggcaactgcg tcatgctatc gggggccgac
     1021 cagtaattat ctttaatgcc c
//
LOCUS       DAZFKF010000327         1034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFKF010000327 DAZFKF010000000
VERSION     DAZFKF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.332"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            40..573
                     /locus_tag="ACHKA1_005554"
     CDS             40..573
                     /locus_tag="ACHKA1_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5683046.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            623..>1034
                     /locus_tag="ACHKA1_005555"
     CDS             623..>1034
                     /locus_tag="ACHKA1_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5683047.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVPFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAGKNKIKLALQNRIKLLWR"
BASE COUNT          345 a          202 c          196 g          291 t
ORIGIN      
        1 aacgagtgac cttccatacc agttaattaa ggatgattta tgaaaaagta tattattccc
       61 ttcgttgcga caacgttgat gtttagcgca acaaccttcg ccaaggatgt cgatcaagga
      121 acactaacca ttagaggaga agttgttgat acaacgtgtt actttgttaa tggtgattcg
      181 acaacagata tcgttgtcga agatgttgta accagtgcaa tgaatcaact tatcaataat
      241 gaaatctata ctttaaaaac cggttcaacc tctcatgact tagaaattca gtgtgacgga
      301 aatacagccc caagtatcga agtactagca tcagagtttg atgctaataa ctttacgctg
      361 aataaaggaa ctgctgagaa cattggcttt gccattttcc tcgatgatgg gactttgacc
      421 agaatgagac cagggttaaa aacaccgctg aaagataatg gctctggtca atattttctt
      481 aatttgaatg cacaatatgc ccgcacaagt ggcaatccag ttacaaaagg ctccgtagag
      541 tcaaccgtaa cgattaaaat cagtgcagat taacgttttt tgccggtagc tctgctaccg
      601 gtattattta cggagtgatt acgtgagaat aataacagca ttactgatgt cattttttat
      661 tttacctgtt catgccggtg tggtcatcta tggtacgcgc attatttatc ctgaaaaaaa
      721 cagagaagtt ctcgtacagt taatgaatca aagtaaaaat gcctctttaa ttcaggcatg
      781 gatcgatgat gggaacacca ctatcgcgcc agaaaaaatt caggtcccat ttattctcac
      841 accgccagta agtcgtgtag caggtggttc tggccaacaa cttaaaataa gaaaaatgcc
      901 aaacaacttg cctcacaata aagaaagttt attttatcta aatgttctgg atatccctcc
      961 aaacaaccca caaaatgcag gaaaaaacaa aattaaactt gcattgcaaa accgtataaa
     1021 gttactttgg cgtc
//
LOCUS       DAZFKF010000328         1020 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFKF010000328 DAZFKF010000000
VERSION     DAZFKF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.333"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..77
                     /locus_tag="ACHKA1_005556"
     CDS             <1..77
                     /locus_tag="ACHKA1_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO5683048.1"
                     /translation="DFTYGYDEDIKEFIKDKTYPYGNW"
     gene            109..375
                     /locus_tag="ACHKA1_005557"
     CDS             109..375
                     /locus_tag="ACHKA1_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO5683049.1"
                     /translation="MSKGFVLLGDNTTHGGKVISASSTMIVDGKKVALVGDMVSCPVS
                     GHGTNPIVEGSPEWISDGRAVVIDGCKCLCGCKVISSAPECAIG"
     gene            379..>1020
                     /locus_tag="ACHKA1_005558"
     CDS             379..>1020
                     /locus_tag="ACHKA1_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO5683050.1"
                     /translation="MGWSRTKSLSTELPHKPSMMLWFMTGAFMAVVGTLLFIIRASGY
                     VKVLNDFSIWWLALTPSGCWFFLFCLRHWQWSNQMDEHLFLKKEGEYAQKQWESWAER
                     YLVITASCVYLPDKITVATLCDELPLQYGLVKKIDYLSDSGHKVEASLRVLLREITDK
                     FCQLPAALPVNVTLITDQPDSEIRSAFVSAWEALFPQRVVPDNIEVTPDFSMGW"
BASE COUNT          267 a          169 c          251 g          333 t
ORIGIN      
        1 gtgatttcac atatggatat gatgaagata ttaaggaatt tatcaaagat aaaacttatc
       61 cttacggtaa ctggtgactc aaagtgaaaa tttatttttg agagttttat gtctaaaggt
      121 tttgtattgc ttggtgataa taccacacat ggtggtaaag ttatttcggc atcttcaacc
      181 atgattgtcg atggtaaaaa agtagctctt gttggagata tggttagttg ccctgtttcc
      241 ggacatggta ccaatcctat tgtggagggc tctcctgaat ggatatctga tggaagagct
      301 gttgtgattg atggatgtaa atgtctgtgt gggtgtaagg ttatatccag tgcaccggaa
      361 tgtgcaatag gataaaccat gggatggagc agaacaaaat ctttatcaac agaattacct
      421 cataaaccat caatgatgtt gtggtttatg acaggggcat ttatggctgt tgttggtact
      481 ctgttgttta ttatccgggc atcaggatat gtgaaagtat taaatgattt cagtatctgg
      541 tggcttgcat tgactccttc ggggtgctgg tttttcctgt tttgcctgcg acactggcag
      601 tggagcaacc agatggatga acatttgttt cttaaaaaag agggggagta tgctcagaaa
      661 cagtgggaat cctgggccga aaggtatctt gttattactg ccagttgtgt ttatttgcct
      721 gataaaataa cggtagctac tctttgtgat gaattgcctc tgcagtacgg ccttgtaaag
      781 aaaatagatt atctttctga cagtgggcat aaggttgaag cctctttacg tgtcttgcta
      841 agggagataa cggataaatt ttgtcagtta cctgccgctc tccctgtaaa tgtgacgctg
      901 ataaccgatc agcctgattc tgaaatcaga tctgcttttg tttcagcatg ggaagcgtta
      961 tttccccaac gcgtcgttcc tgacaatatc gaggttactc ccgacttttc catggggtgg
//
LOCUS       DAZFKF010000329         1002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFKF010000329 DAZFKF010000000
VERSION     DAZFKF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.334"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(100..>1002)
                     /locus_tag="ACHKA1_005559"
     CDS             complement(100..>1002)
                     /locus_tag="ACHKA1_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5683051.1"
                     /translation="ISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYVNLDP
                     VIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNVTYP
                     FPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLGSGN
                     PRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLSFYLS
                     GATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENISLGTVNKSKVPLGLSAT
                     YGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT          279 a          231 c          216 g          276 t
ORIGIN      
        1 gcgcggattt tcttacaggt gtaggataat agccttcaca aaataattat ccgcgcaatt
       61 ttcgtgaatg gagcgcatag accaatgtca aaatcacaat tattcataga taaaagtgac
      121 accaatgact gactggacgg ttccggctga aaccttgttt cctgtttgtc cataggttgc
      181 gcttaaaccc agaggtactt tgcttttatt tactgtcccc agggagatat tttctcccgt
      241 agccagtatt tttccattac gcatcagaga gacacccact cccgatgctt ttgttgcatc
      301 aggtgctgtg ttagcaaaca cttgtcgggc actgtcagtg gtggcacccg aaagataaaa
      361 tgagagcttt tgttcgcttg agcaatatac gccaaggggg atttctgcgc ttccaggaaa
      421 gtccgggaga ttcacggtga cattacgact atcaacagtg cagccgcctg ttggcataac
      481 aacactgtta tttgaaataa tattccacgt aaaattacgc ggatttccgc ttcctaacgt
      541 ggcaatttta tacatatgaa tacgggcaat aacttccccg gcttttatga caactccacc
      601 ggccgccccg actggggtaa tatagagttt taacggtaat ggcatcggtg tcttatcacc
      661 aatatccaat acgttggtat ttgtcgtcaa cgggaagggg taggtcacat tattccagta
      721 cagcgatcct ttatatgact gtagagagcc tgcgaaagct gagccttgta ccaggtttat
      781 atgatcagtg tcgtaccagc cgccgtaatc attccagcaa cttatatgct gagacaagtc
      841 tacaaccaga ttctggcccg gctgtattac agggtcgaga ttaacataaa cagaggttgt
      901 accagctcca atacttgacc cgccatcgac attacaactg aaggcaaaaa ctttgcctgc
      961 cattaataga agataaatac cgaacaggac ctttatactg at
//
LOCUS       DAZFKF010000330          991 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFKF010000330 DAZFKF010000000
VERSION     DAZFKF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.335"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>991
                     /locus_tag="ACHKA1_005560"
     rRNA            <1..>991
                     /locus_tag="ACHKA1_005560"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          270 a          215 c          315 g          191 t
ORIGIN      
        1 ctggaggacc gaaccgacta atgttgaaaa attagcggat gacttgtggc tgggggtgaa
       61 aggccaatca aaccgggaga tagctggttc tccccgaaag ctatttaggt agcgcctcgt
      121 gaattcatct ccgggggtag agcactgttt cggcaagggg gtcatcccga cttaccaacc
      181 cgatgcaaac tgcgaatacc ggagaatgtt atcacgggag acacacggcg ggtgctaacg
      241 tccgtcgtga agagggaaac aacccagacc gccagctaag gtcccaaagt catggttaag
      301 tgggaaacga tgtgggaagg cccagacagc caggatgttg gcttagaagc agccatcatt
      361 taaagaaagc gtaatagctc actggtcgag tcggcctgcg cggaagatgt aacggggcta
      421 aaccatgcac cgaagctgcg gcagcgacac tatgtgttgt tgggtagggg agcgttctgt
      481 aagcctgtga aggtgtactg tgaggtatgc tggaggtatc agaagtgcga atgctgacat
      541 aagtaacgat aaagcgggtg aaaagcccgc tcgccggaag accaagggtt cctgtccaac
      601 gttaatcggg gcagggtgag tcgaccccta aggcgaggcc gaaaggcgta gtcgatggga
      661 aacaggttaa tattcctgta cttggtgtta ctgcgaaggg gggacggaga aggctatgtt
      721 ggccgggcga cggttgtccc ggtttaagcg tgtaggctgg ttttccaggc aaatccggaa
      781 aatcaaggct gaggcgtgat gacgaggcac tacggtgctg aagcaacaaa tgccctgctt
      841 ccaggaaaag cctctaagca tcaggtaaca tcaaatcgta ccccaaaccg acacaggtgg
      901 tcaggtagag aataccaagg cgcttgagag aactcgggtg aaggaactag gcaaaatggt
      961 gccgtaactt cgggagaagg cacgctgata t
//
LOCUS       DAZFKF010000331          988 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFKF010000331 DAZFKF010000000
VERSION     DAZFKF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.336"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            48..>988
                     /locus_tag="ACHKA1_005561"
     CDS             48..>988
                     /locus_tag="ACHKA1_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5683052.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAG"
BASE COUNT          313 a          178 c          239 g          258 t
ORIGIN      
        1 taaccagtga aggtattaca ggaaaagtgt gagtaagagg agctattttg tttcgtcagg
       61 atgctttaga aaacagaaaa atgaagtggc agggacgggc aatattactt cccggaatac
      121 cactatggtt aatcatgctg ggaagcatag tgtttattac ggcatttctg atgttcatta
      181 ttgttggtac ctatagccgc cgtgttaatg tcagtggtga ggtcacaacc tggccaagag
      241 ctgtcaatat atattcaggt gtacagggat ttgttgtcag gcagtttgtt catgaaggac
      301 agttgataaa aaaaggggat cctgtttatc tgattgacat cagtaaaagt acacgtagtg
      361 gtattgtcac tgataatcat cgtcgggata tagaaaacca gctggttcgt gtggacaaca
      421 ttatttcccg tctggaagaa agtaaaaaaa taacgctgga taccctggaa aaacaacgcc
      481 tgcaatacac agatgcgttt cgtcgctcat cagatattat acagcgtgca gaggaaggga
      541 taaaaataat gaaaaacaat atggagaatt acagaaacta tcaggcaaaa gggctgatta
      601 ataaagatca gttaactaac caggtggcat tatattatca gcaacaaaac aatcttctca
      661 gcctgagcgg acagaacgaa cagaatgccc tgcagataac cactctggag agtcagattc
      721 agactcaggc tgcagatttt gataaccgta tctaccagat ggaactgcaa cggtacgagt
      781 tacagaaaga actggttaac actgatgtgg agggcgaaat cattatccgg gcgttgactg
      841 acgggaaagt tgactccctg agtgtcactg tcgggcaaat ggtcaatacc ggagacagcc
      901 ttctgcaggt tattcctgag aacattgaaa actattatct tattctctgg gtcccgaatg
      961 atgctgttcc ttatatttcg gctggtga
//
LOCUS       DAZFKF010000332          959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFKF010000332 DAZFKF010000000
VERSION     DAZFKF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.337"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            33..107
                     /locus_tag="ACHKA1_005562"
     CDS             33..107
                     /locus_tag="ACHKA1_005562"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683053.1"
                     /translation="MSQQVQRLAGQVDRLSRLFITNSR"
     gene            complement(597..842)
                     /locus_tag="ACHKA1_005563"
     CDS             complement(597..842)
                     /locus_tag="ACHKA1_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683054.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRAR
                     ILKAILDERTASKNREATLTAGQDPDDTLTWQPLRRR"
BASE COUNT          191 a          216 c          287 g          265 t
ORIGIN      
        1 agaacgggca actgagggaa cagtgcgacg acttgagtca gcaggtgcag agattagccg
       61 ggcaggtgga ccgcttgagt cggttattta tcacgaacag cagataaggg atgaacagga
      121 aaggcaggca cgcctggaac gggagcgtgc gcggtcctgg catgaagagc aggaacggga
      181 agcacgggta tggcgtggcc cgtcaatggg gatgtgacgg tgagttgttc cgggaggttg
      241 ccagatggta ccggaacgcg ttaaaaacga atccgggcgc gattctggcg tgtcgttttt
      301 gttgctgtgt tttgcctctt tgccagatcg cgctcattcc tcagcctcat cccgcgatgt
      361 ctgcgtatgt catcaactga aaagatggaa aaaaaacagt gtcatgaggt ttacgcaggc
      421 aaaaccagcg ttattcacat ggctgaatag ctatgcatac ggttaaaatt tatcaggtgc
      481 gatcgcggca gcttttcggg tggtttgttg ccatttttac ctgtcagcag ccgtgatcgc
      541 gctgaacgca tttcagcggt gcgtacaata aggggttatg ttaaataacc cttagcttag
      601 cggcggcgca gtggttgcca ggtgagggtg tcatccgggt cctgtccggc agtcagtgtg
      661 gcttctcgat ttttgctggc cgttcgttca tcaagaatgg ctttaagtat ccgtgcccgg
      721 aggcgtcgct ttgtttcctg cggcattttg tcctttttcg tcagccccat ggcacgagct
      781 tcagcggcaa tggcttctgc cagtcggcgc tcttcggctt cggtcagtct atggcgtttc
      841 atggtggtct ccgggtgttt ctctggtgtc ataacagttt aactgttggc tctctcctta
      901 tgctaatcag ccttctttca tgcctgttgc cattgagttt aagaatgatt ggttgtttt
//
LOCUS       DAZFKF010000333          959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFKF010000333 DAZFKF010000000
VERSION     DAZFKF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.338"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..355)
                     /locus_tag="ACHKA1_005564"
     CDS             complement(<1..355)
                     /locus_tag="ACHKA1_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683055.1"
                     /translation="MHSLLILLYVVVWRACIILRVIAPVSAVRAWCEYQLEGMTILRD
                     AYSSLDKAAGMQNDAFEYTESIARHLGYKSGGDQLKSGLFRPFLWHIRVKGHRLGAVM
                     EMEAWKTWKNGCNDVC"
     gene            complement(438..830)
                     /locus_tag="ACHKA1_005565"
     CDS             complement(438..830)
                     /locus_tag="ACHKA1_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683056.1"
                     /translation="MSTFILCPEPGISSFFRRMTAQLRVFFSRNNLSYYSRWYTNPAG
                     NLSVRVCMFRRGGVWTPCLYYRQGMYRNEYTGWYQPLPREVIYLNRFTGRISRRRTWH
                     AACRMAEQLTTLRYRFGLQGDGIVAQPC"
BASE COUNT          276 a          269 c          220 g          194 t
ORIGIN      
        1 tgcagacatc gttacagcca ttcttccatg tcttccatgc ctccatctcc atgacagccc
       61 ccagcctgtg gcctttcaca cgtatatgcc acagaaacgg ccggaaaagt cctgatttca
      121 gttggtcacc accgcttttg tagcccagat gacgtgcaat actttcagta tattcaaatg
      181 catcattctg cattccagcc gccttatcca gcgagctgta cgcatcacga agtatcgtca
      241 taccttccag ttgatactca caccacgcac ggacagccga cactggtgcg attacccgaa
      301 gaatgatgca ggcacgccat acaacaacgt aaaggagtat taacagacta tgcatgtgtt
      361 cacctcagat acaaaaaata acgggaacac acctgccctg cgggaagcgt gttcccgaca
      421 gggtaataaa tgataaatca gcagggctgt gcgacgattc catcaccctg cagaccaaac
      481 cggtaacgca gggtggtcag ttgctccgcc atacggcagg cagcatgcca tgtcctgcga
      541 cgggatatcc gtccggtaaa ccggttcaga taaatgacct cacgcggtaa cggctgatac
      601 catcctgtgt actcgttgcg atacattccc tgccggtaat acaggcaggg tgtccagaca
      661 ccgccacgac ggaacataca gacccgcacg ctcagattgc cggccggatt agtgtaccag
      721 cgactgtaat atgacaggtt attgcgggaa aaaaacaccc gcaactgagc cgtcattcgc
      781 cggaaaaaag aagagatgcc cggttcaggg cacaaaataa acgttgacat aaagactcct
      841 gtaaaaaaac gggagccttc gccccgacgg acaaaaactc ccgtcagggt taaaacacca
      901 ccagtacagc atggtgttgc tgtcagaaaa agcccgttaa taaagggcgc agttataaa
//
LOCUS       DAZFKF010000334          951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFKF010000334 DAZFKF010000000
VERSION     DAZFKF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.339"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(680..>951)
                     /locus_tag="ACHKA1_005566"
     CDS             complement(680..>951)
                     /locus_tag="ACHKA1_005566"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683057.1"
                     /translation="KINIKNSAKFNIRSTSRLTYDIISQLHLPQNGYFMERSNFLVAI
                     IMLILRSLAIIPGWFVSLGHEIVTFLFIMEIWGKAGNDFFIYIRW"
BASE COUNT          285 a          169 c          188 g          309 t
ORIGIN      
        1 cctgagtata aaatctcttc tgatgtttaa ttgattttaa tgttcgtaag ctatatcact
       61 tactcaatcc attttaccca gagtcataaa atagagtgtc tgttgatctc attttcatat
      121 tcctttcaga ctgaagtgcg ttttcatcac ccgatgagaa agaactgaag tgatgtttgc
      181 catagattgc aagatgccct gtctcattgt ctattgtggt gcacctttat acctgttata
      241 ccagatcaaa aatcacgcaa tccatacaac aaaaccagat ttgcaattcg tgtcacaaaa
      301 tatgtcgatc tttttctaag aagaagatgc catgtgaagc cagacgaaca cttgcggtgg
      361 tctttaaaaa ctaaagatct tcatttaact atttgtttta taaataattt atttagagtc
      421 taaacaaggg aagctttgca agctaactca gtgggcgtgg tgaaaataag tgtttacccg
      481 ccatcaggct gagcataatt ctcatcatga aatatgtttc ctggtttgtg gcttgtaact
      541 ggtcacttct gaagtcgatc tggagaggct tgttgatgtt ggtgttttca ggatgatgtt
      601 tcagatagtt tgtttgccgt atcgcccggc gaatggctgt gattgaggaa ggtttaagtc
      661 gtagtgacca aagctatatt taccaacgaa tgtagatgaa aaaatcgttt cctgctttcc
      721 cccatatctc catgataaaa aggaatgtaa caatctcatg gcctaaactg acgaaccagc
      781 caggaataat cgctagggac ctaagaatta gcatgataat agccactaag aaattactgc
      841 gttccatgaa atagccattt tgtggcaaat ggagttgact aataatgtca tatgtgagac
      901 ggctagttga acgaatatta aattttgctg aattttttat gttgatttta c
//
LOCUS       DAZFKF010000335          945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFKF010000335 DAZFKF010000000
VERSION     DAZFKF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.340"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..470
                     /locus_tag="ACHKA1_005567"
     CDS             <1..470
                     /locus_tag="ACHKA1_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HFO5683058.1"
                     /translation="QKNSNNKDREKKYRDGDKINGPQAHSLDSTNERRFADNRTMFTQ
                     HIEKQRNVHTLNQNDINNSVNSVNVPENELTYQFQRWGQNHTVRILESSEGIRLKPSD
                     ALVSDRLHEAQNNDVTAQRWVLTEQDERQGQRHQPHEEQENEGKFENDQKDES"
     gene            430..945
                     /locus_tag="ACHKA1_005568"
                     /pseudo
     CDS             430..945
                     /locus_tag="ACHKA1_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT          341 a          164 c          207 g          233 t
ORIGIN      
        1 atcaaaaaaa cagtaataat aaagatcgtg aaaaaaaata ccgcgatgga gacaaaataa
       61 atggaccgca ggctcattca ttagacagta ctaacgaaag aaggtttgct gataacagaa
      121 ccatgtttac tcaacatatc gaaaagcaaa ggaatgttca tacgctcaat cagaatgata
      181 taaataattc cgttaatagt gtcaatgtgc cagaaaatga attgacatat cagtttcagc
      241 gttggggaca gaatcataca gtccggattc tggaaagtag tgaagggata cgactaaaac
      301 catcagacgc gcttgtctcc gacagactcc atgaagccca aaacaatgat gttacagcac
      361 aacgttgggt attgactgag caagatgaac gacaaggaca acgacatcag cctcatgagg
      421 agcaagaaaa tgaaggcaaa ttcgaaaacg atcagaagga tgaaagttaa tgttcggtct
      481 gaggaaagta aatcgaaaca ctcatacgtt tgaaaccact tttcaaaatt ggaaagaaaa
      541 tggcgaagat gttgcattat taatgcccga atttagtgct aaatggctac caatagcaga
      601 agagagtggc agctggtctg gatgggtatt gcttcgagaa atatttccct taatttcttc
      661 tgagctcgct gggatggctt taatgccaga aacggaaagg ttaatagggg aatggctcag
      721 tttatccagt tccccgttaa atctaaagca tcctgaacta aaatataatc gtttgtgcgt
      781 aggtaaagta tttgatggag tattgagccc agctcaacca ctaataagaa tatggacagg
      841 ggaattgaat ctttggttag ataaagtcac agtctgccaa tacggaaacg ctccagcgtt
      901 agacaaaaaa tcattatatt ggcccattca ttttgtaatt ggatt
//
LOCUS       DAZFKF010000336          933 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFKF010000336 DAZFKF010000000
VERSION     DAZFKF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.341"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            81..881
                     /locus_tag="ACHKA1_005569"
     CDS             81..881
                     /locus_tag="ACHKA1_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683059.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVARAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKTSNKELSNDHVTEAQLFNLKQKAYVSMVKDLVSRFQ"
BASE COUNT          294 a          178 c          214 g          247 t
ORIGIN      
        1 tttaagtggg gtatataatt agagtccgtt atcaatgcta aatattctaa tcattatgac
       61 aggcgaggga gtgtccaatt atgaattcaa aaaagctttg ttgcatatgt gtgttattct
      121 cgctgcttgc aggatgtgcc tctgaatctt ctattgatga aaagaagaaa aaagctcaag
      181 tcacacaaag taatattaat aaaaacactc cccagcaact gacagacaaa gatttattcg
      241 gtaatgaaac cactctggcc gtatccgaag aggatattca agcagcgctt gatggagatg
      301 agttccgcgt tccccttaat tctcctgtaa tacttgttca atccggcaac cgcgcaccgg
      361 aaaccattat gcaggaggag atgcgaaaat attatactgt ttccacattc tctggtatcc
      421 cggacaggca aaagcctctg acttgtaaca aaaacaaaga taaaaatgag aacgaggatg
      481 ttgcccgtgc tgagaatatg aactggatgc aggcactgcg ttttgtggct gcaaaaggac
      541 atcagaaagc gattattgtt taccaggata tgttgcagac aggaaaatat gactctgcgc
      601 tgaaatcaac agtatggtcc gactataaaa atgaaaaact cactgacgct atctctctgc
      661 gctacctggt acgtttcacg ctggtggatg tggcaacagg tgagtgggct acctggtcgc
      721 cggtgaatta tgaatcgaga gtaatttttc cgccggctgg aattacaaaa acgagcaata
      781 aagaactatc aaatgaccat gttacggaag cgcaactgtt taatttgaaa caaaaagcat
      841 atgtatcgat ggtgaaagac ttggtgagtc gattccagta atcgttattg ttatgctgcc
      901 gcctgtagtt tagggtggca gcaaacagga gtt
//
LOCUS       DAZFKF010000337          920 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFKF010000337 DAZFKF010000000
VERSION     DAZFKF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.342"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..392
                     /locus_tag="ACHKA1_005570"
     CDS             <1..392
                     /locus_tag="ACHKA1_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO5683060.1"
                     /translation="CIENGINSTPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSFNGL
                     GISVHDISAQKISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWF
                     FLQRHKDFAFKPFFTNFNTIERIENYL"
     gene            389..880
                     /locus_tag="ACHKA1_005571"
     CDS             389..880
                     /locus_tag="ACHKA1_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO5683061.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGSSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNLHDFKK
                     DNF"
BASE COUNT          323 a          162 c          143 g          292 t
ORIGIN      
        1 aatgtatcga aaatggcatt aattcaactc cctcgacagg ataccaacct cttatcctgc
       61 aaagcattaa aacaaaatta ctttcatcaa gactaaataa atttaatgac ttcgaggaca
      121 gttttaatgg cttaggaatc agtgttcatg atatttcagc tcaaaaaata tctcttttaa
      181 gttttcaaaa atacgctata ggatggagtg cgacaattca ttttgtagca caggaccact
      241 tcggtcttga tgtaactgat atcaaaaaca agacttacag caaatataga ttttttcgca
      301 tatggttctt tttacaacgg cataaagact ttgctttcaa accttttttc accaatttta
      361 acactatcga aagaatcgaa aattatctat gaaaagcaaa ctccagaaaa ttatattatg
      421 tttatttctt ctatgctgca tctataattt atggacattg cgtcctgtac agattttgta
      481 tacatactct gatgctggca gttcagtctt tctggtcgtt gatcatctgc catggactga
      541 cagcgataaa ataaattggt atctgaagca tcagaatgaa attaaaaatc aacatcccct
      601 accagaaggt agttggcata cctggtatgt cattgatatc ggtaatggct tcactgacta
      661 taaaaaatat atcgaagggc cgtatgaaga tctctactgt ttcccgacaa ttaagagcaa
      721 tgataattgc atcgtgaaaa attacttaat ggttattaat gaataccctt atcgtaacac
      781 tcatattggt attaacgatt ttactgagta tcaattaaca caagaaaata aaatagagcg
      841 agttttcaat ctgcatgact ttaaaaaaga caatttttag aaataagccg gataaaaaaa
      901 tagctttatc cggcactcat
//
LOCUS       DAZFKF010000338          914 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFKF010000338 DAZFKF010000000
VERSION     DAZFKF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.343"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..488)
                     /gene="sctT"
                     /locus_tag="ACHKA1_005572"
     CDS             complement(<1..488)
                     /gene="sctT"
                     /locus_tag="ACHKA1_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFO5683062.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLGIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGMNFILETLWQSYNLWPSG
                     NF"
     gene            complement(490..750)
                     /locus_tag="ACHKA1_005573"
     CDS             complement(490..750)
                     /locus_tag="ACHKA1_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO5683063.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(759..>914)
                     /locus_tag="ACHKA1_005574"
     CDS             complement(759..>914)
                     /locus_tag="ACHKA1_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO5683064.1"
                     /translation="ISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQY
                     FDLSINP"
BASE COUNT          319 a          195 c          157 g          243 t
ORIGIN      
        1 ttaaaattac ctgaaggcca taaattataa ctttgccaaa gagtttccag aataaagttc
       61 attccaccgt tagtaagata aaccgcagca gagaaaaggt taaataatct tgctaattcc
      121 gaggtatcga cacctgtagc cggatccaaa gtactactta atgttgcacc gcgttggtta
      181 tcgataaagc tacctatacc aaggaaaatc caaaatgggg atgctaataa gcatgcaata
      241 aacagcccta tgataatttc tctggcagcg aacaaagcaa tatcgatttc atttaagttc
      301 aataattcat agtggtaatg ctgaactaat gcacatgata ccactattat tattggatga
      361 cggactactg ttggtatatt cccactggcg aaaaatggta acaaatagaa agtgggtgcc
      421 aatcggcaaa aaccgagcgc tgtagcagcc agcagactat gcaactgata gagtatcgcc
      481 tcacccatgc tatcctctcg cgaatgctaa attcagcatt tcaataccaa aagaatataa
      541 tttttcgcca taccatccgc tcattaaaaa aaagcaaata cttacgcata ataatttcac
      601 gccaaatggc aacgtctgtt cttgcaactg tgttaccgtt tgaaaaagcc ctactaataa
      661 accaacaaat gtcgcaactg cgataggtcc tgcagacata actaaaatca gatacagcgc
      721 cctattccct gcaaaaacaa tatcgtccat attttttctc agggattaat ggataggtca
      781 aaatattgta aaatgagtcc ttttgataac attgtccaac cgtccatagc gacaaacaga
      841 attaatttta tcggtgtaga tatggttacc gggctcatca tcatcatacc taacgttaac
      901 aaaacgctgg atat
//
LOCUS       DAZFKF010000339          907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFKF010000339 DAZFKF010000000
VERSION     DAZFKF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.344"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..265
                     /locus_tag="ACHKA1_005575"
     CDS             <1..265
                     /locus_tag="ACHKA1_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO5683065.1"
                     /translation="MHIPMLKKRGIIQDEQDLLDNPCLNIKIGTEILYNHFSRCGVTW
                     QCLGTYNAGFAMDNKKKRLQYAKKIYIVYTRLNELDNRKALAK"
     gene            complement(283..906)
                     /locus_tag="ACHKA1_005576"
     CDS             complement(283..906)
                     /locus_tag="ACHKA1_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO5683066.1"
                     /translation="MKIVVSDQQPFMIDGIIGFLGHYPDLYEVVGGYKDLKKAIAECN
                     KSAAQIFILGEFAGGMMGSELVKWVKSHKIDAHIITFVAKMPYIDSIKLLEAGAKGCV
                     WKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIADGK
                     TNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
BASE COUNT          291 a          178 c          146 g          292 t
ORIGIN      
        1 gatgcacatt cccatgttaa aaaaacgtgg aataatacaa gatgaacaag atttgttaga
       61 taacccctgt ctgaacatta aaataggaac cgagatttta tataaccatt tttcacgttg
      121 tggagtaacg tggcaatgcc tggggactta taatgcgggt tttgcaatgg ataacaaaaa
      181 aaagagactg caatatgcca aaaaaatata cattgtatat accaggctta atgaactaga
      241 caatcgtaag gctttagcaa aatgagccta aagcctcttt ttttatatag tgcaaacacc
      301 catacgtaaa gctgttttga tcaactcgat accactgtga acatctaatt tcttcatgat
      361 attgagtcta tgagtttcga ctgttttcct gcttaactgc aagaaattag cgatttcttt
      421 gtttgtttta ccatcagcta tgagttgtaa tatttctgac tcacgatttg taagttgatt
      481 atcagaggaa tacctggaag atattttttc acaatccata tgtacactat caaaataagt
      541 gtagccatta ctaatcgaat caatagcgcg atttagtttc gccgggtgac tggttttcca
      601 tacgcatcct ttcgcacctg cttcaagcaa ttttattgaa tcaatatacg gcatctttgc
      661 tacaaatgta ataatatgag catctatttt atgcgattta acccatttta cgagttcgga
      721 ccccatcatt ccacctgcaa attctcctaa aataaatatt tgggctgcag atttgttaca
      781 ctccgctata gctttcttca gatctttata gcccccaaca acctcatata aatcaggata
      841 atgtccgagg aatccaatta tcccatcaat cataaacggc tgctgatctg aaactacaat
      901 tttaatt
//
LOCUS       DAZFKF010000340          905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFKF010000340 DAZFKF010000000
VERSION     DAZFKF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.345"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>905
                     /locus_tag="ACHKA1_005577"
     CDS             <1..>905
                     /locus_tag="ACHKA1_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO5683067.1"
                     /translation="TEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEY
                     GLNVVSGDITDITTPDGRETKFYYNDGNLVTAVVYPDGLESRRAYDERDRLVTETSRS
                     GETVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQM
                     TAVHREEGISLYRRYDNRGRLTSVKDAQGRETRYEYNAAGDLTAVITPDGNRSETQYD
                     AWGKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQR
                     YHYDLTGKLTQSEDEGLVTLWHYDE"
BASE COUNT          217 a          249 c          313 g          126 t
ORIGIN      
        1 tacagaaggc ggggccgggc tgaagcgggt ggtgaaaaaa gaactggcgg acggcagcgt
       61 cacgcacagc gggtatgacg cggcaggaag gctcacggcg cagacggacg cggcgggacg
      121 gcggacagag tacggtctga atgtggtgtc aggcgacatc acggacatca ccacaccgga
      181 cgggcgggag acgaaatttt actataacga cgggaacctg gtgacggcgg tggtgtaccc
      241 ggacgggctg gagagccgcc gggcgtatga tgagcgggac aggctggtaa cggagacatc
      301 gcgcagcggg gagacagtac gctaccgcta tgatgatgcg cacagtgaat taccggccac
      361 aacaacagat gcgacgggca gcacccggca gatgacgtgg agccgctacg ggcagttgct
      421 ggcgttcacc gactgctcgg gctaccagac ccgctatgaa tacgaccgct tcggccagat
      481 gacggcggtc caccgcgagg aaggcatcag cctttaccgc cgctatgaca accgtggccg
      541 gttaacctcg gtgaaagacg cacagggccg tgaaacgcgg tatgaataca acgccgcagg
      601 cgacctgact gccgttatca ccccggacgg caaccggagc gagacacagt acgatgcgtg
      661 gggaaaggcg gtcagcacca cgcagggcgg gctgacgcgc agtatggagt acgatgctgc
      721 cggacgtgtc atcagcctga ccaacgagaa cggcagtcac agcgacttca gctacgatgc
      781 gctggaccgc ctggtacagc agggcggctt tgacgggcgg acgcagcgtt atcattatga
      841 cctgaccgga aaactcacac agagtgagga tgagggactt gtcaccctct ggcactacga
      901 cgaat
//
LOCUS       DAZFKF010000341          893 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFKF010000341 DAZFKF010000000
VERSION     DAZFKF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.346"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            194..796
                     /gene="fimB"
                     /locus_tag="ACHKA1_005578"
     CDS             194..796
                     /gene="fimB"
                     /locus_tag="ACHKA1_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO5683068.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          264 a          164 c          194 g          271 t
ORIGIN      
        1 agcatagcgt ttgtatggca acgttattat aattaactgt tgctaatcca tttaagttca
       61 ctcagaagaa ctggtccact tacgttagtt attaagcaaa cgttcgcttt tataaacata
      121 atcaggataa aaatgttgga ttattgctaa cccagcacag ctagtgcgcg tctgtaatta
      181 taagggaaaa acgatgaaga ataaggctga taacaaaaaa aggaacttcc tgacccatag
      241 tgaaatcgaa tcactcctta aagcagcaaa taccgggcct catgcagcac gtaattattg
      301 tctgactttg ctttgtttta ttcatggttt ccgggcgagt gaaatttgtc gattgaggat
      361 ttcggatatt gatcttaagg caaagtgtat atatatccat cgattaaaaa aaggcttttc
      421 aacaacgcac ccgctattga acaaagaagt tcaggcttta aaaaactggt tgagtatccg
      481 tacttcgtac ccgcatgctg agagcgagtg ggtattttta tcgcgtaagg ggaatccgct
      541 ttctcggcaa cagttttacc atattatctc gacttccggt ggtaatgccg ggttgtcact
      601 ggagattcat ccgcacatgt tacgccattc gtgtggtttt gctttggcga atatgggaat
      661 agatacgcga cttatccagg attatcttgg gcatcgcaat attcgtcata ctgtctggta
      721 taccgccagc aatgcagggc gtttttacgg catctgggat agagccagag gacgacagcg
      781 tcacgctgtt ttatagagat agttgagata ccagggatgg tgttaaaaac tacattattc
      841 ttacgatata tcaaaaatga ttaaccattt attaattgat gttattgtaa gta
//
LOCUS       DAZFKF010000342          881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFKF010000342 DAZFKF010000000
VERSION     DAZFKF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.347"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..590)
                     /gene="vgrG"
                     /locus_tag="ACHKA1_005579"
     CDS             complement(<1..590)
                     /gene="vgrG"
                     /locus_tag="ACHKA1_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO5683069.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIP"
BASE COUNT          258 a          213 c          209 g          201 t
ORIGIN      
        1 gggatttcaa aagattcggg cagatggcgg accgtgtcgc acagcaccag gctctggtcg
       61 gtacttttgt aagcatgctc ctcataaaag aagatgcctt cctccgccgc catccgacac
      121 aggaaatcgt aatcagtctc accgtactgg acacaaaact cacgggaagg atgcggttca
      181 ctgaacagcg gactccactc ggtcaccccg ttttcctgca atatcgtgcc gaggatgctt
      241 ttgatgtcct cgttctggaa gatacggaag ttctggcgca gaccggcacg ccacagcggc
      301 gggtgcacct tcatgctgta cagcatctgg tttttgtcgt tctcccccag ttcaaaccag
      361 gtcaccacac ctttcacccg gcgctgtaca tcatcaccct gccatattgt caggtaggcc
      421 attttgtcca gcacctgcgc aaattcaagg gagagaaact gctggctgac cagggagaga
      481 tcgagggaaa agagcgaaga gagtgactgg gtgagatgaa aggaaaccac cgcaaaagcg
      541 tccggtggca ggccgtccac ttccagtgtg aaacgtaatc cggttgacat agcaacctcc
      601 ggtaaaatga ataataatcc gcaccgtaca actgcggata atgagataag cgggacgact
      661 taagacgtta ttagggaagg aacaaaaaat gctatgaata tgtgagaatt ttgtttaaat
      721 attactttac agaaacgggt gtctgttcag tcaaaaaaat aatcacaagc tcaacttatt
      781 gtttcctgac gttattcagg acaggtgcgc acatcacagc gagtaacaaa aacgcaaagt
      841 aaaaccaata ttcataatat gtttttattc acaactaaat a
//
LOCUS       DAZFKF010000343          861 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFKF010000343 DAZFKF010000000
VERSION     DAZFKF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.348"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(8..235)
                     /gene="mchB"
                     /locus_tag="ACHKA1_005580"
     CDS             complement(8..235)
                     /gene="mchB"
                     /locus_tag="ACHKA1_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HFO5683070.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALAGIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
     gene            complement(263..472)
                     /gene="mchI"
                     /locus_tag="ACHKA1_005581"
     CDS             complement(263..472)
                     /gene="mchI"
                     /locus_tag="ACHKA1_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HFO5683071.1"
                     /translation="MSYKKLSQLTAIFSLPITILLVSLSSLRIVGEGNSYVDVFLSFI
                     IFLGFIELIHGIRRILVWSGWKNGS"
BASE COUNT          283 a          203 c          152 g          223 t
ORIGIN      
        1 aatataatta gctaccgcca ccagcagaag aactggcact accacttccc acggtcgagc
       61 caattgctgt tgtcaaaccg gcagatacgg ctccaactac aaccccaagt ggaccaccag
      121 gtattccggc gagagctcca acaatagctg cagcacctgc agcattagct gaagtcgctg
      181 gcgcacctcc cgccccggaa atatatctta actgtgattc tgttatttct cgcatataaa
      241 ctgcatataa actccatcat atttaacttc cgtttttcca gcctgaccag accagaatcc
      301 ttcgaatccc atgaatcagc tcaataaaac caagaaatat tataaagctt agaaaaacgt
      361 caacataaga attcccttcg ccaacaatcc gaagggatga aagtgaaacc aataagatag
      421 taataggtaa actgaatata gccgtcaatt gggacagttt tttataactc attaatccct
      481 acctgttgct ttattttcat tatgaaaagt taccaaatct tagcacctca gtcaggagag
      541 tcaggtcatc gataaagaat attccagtag agtggcgctc atgatcaaag ataacagcaa
      601 agttgatgac agtgctgacc gactgtcatt tacagaaacc ctgtttgtag cacattccat
      661 cttacctgac ctcacccaga caaagacaaa agagaatgat tgttatttac ttttcaaaaa
      721 aaagttgtcc atataaagtc aagaggtatt ttacgttatg tcactgatgc aattgttagt
      781 caggatctca tggttgtcgc tcatgtataa cctgagactt gaacgcacac taataacact
      841 gacggtcata catcgggaaa t
//
LOCUS       DAZFKF010000344          851 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFKF010000344 DAZFKF010000000
VERSION     DAZFKF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.349"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(189..731)
                     /gene="fimA"
                     /locus_tag="ACHKA1_005582"
     CDS             complement(189..731)
                     /gene="fimA"
                     /locus_tag="ACHKA1_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5683072.1"
                     /translation="MKLRFISSALAAALFTATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          264 a          172 c          163 g          252 t
ORIGIN      
        1 aaaataagaa atataaagaa atgattgctt tggtttctta caacacatca ttcccttttc
       61 atttaataaa gcggcatgat acaaagtgaa catgatttcg atttacattg ccaccatacc
      121 gcttcagaca accggtacat tactttgtta taagacgtga tatataattc aaaacaacgt
      181 ggttttgatt attcgtatct cataataaaa gtcgcgtcag catttgcttg ccctgctgat
      241 gcacttgtac cggtgccttt ataacgtgca gaaaaatgaa gaacgttggt cccggggatc
      301 aagttctggt tggttgagaa gctattccca tcaggtttga gaattgcgga tgtattatca
      361 agtatttcaa taccgacacc cgttgctgtt gttgtattgg tactggatgc aatggccaat
      421 aaattatcat tgattgcatc agcctgacca gaaaatgcaa cggcagcatt agcggcaaca
      481 acaggatcgc agtcgttcaa ctgtatagtg aatggaatta atgcagaggt attaccaaca
      541 gcattgaaaa tatcggtacg atattgaccg agtgtgacaa cctggtctgc cgagtcagta
      601 ttcactgaac aggcagcatt caccagttcg ccttcaaagt gaattgtacc gccatctaca
      661 acggcagcat aactacccgt agcggtgaat agtgcggcag ccagcgcaga cgaaataaat
      721 cttaatttca tatatattcc ttcaatctca tttatcgact ccacatccgt atataaccga
      781 ttactttatt taagacactg atagtagtaa attccttttt atcctctaag aatgtcttaa
      841 ttgaaaatat g
//
LOCUS       DAZFKF010000345          843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFKF010000345 DAZFKF010000000
VERSION     DAZFKF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.350"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..445)
                     /locus_tag="ACHKA1_005583"
     CDS             complement(<1..445)
                     /locus_tag="ACHKA1_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683073.1"
                     /translation="MRDKTREAMRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQR
                     ASRLAASVKRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVF
                     GEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLY"
     gene            complement(562..>843)
                     /locus_tag="ACHKA1_005584"
     CDS             complement(562..>843)
                     /locus_tag="ACHKA1_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO5683074.1"
                     /translation="IRILKQTAAAHSDPAYWLEEMTVYCAMELAAGYYGATNRYGTIS
                     LASAASQAGLTWEGLAHSAIADARMTAGVVNAIAAYHLELLQEQARLEI"
BASE COUNT          187 a          244 c          201 g          211 t
ORIGIN      
        1 gataaagccg ctttacccgt tcaatttctg ccagcgttgc ggaccagtgt agttcggcgg
       61 catgacgata tctggcagcc attttttcca ccatctccgg attgctgtcc gcgctgcggt
      121 gctgacgccc cttctcccca aaaacttcaa caaccagcca ctggcttccg gttctgccga
      181 acaatgcctt gcacaaacgt ctcacagtca gcggggttaa atctggatcg ggatcgtagg
      241 caatggtagc aaaaataaag cgcagcattt ctgatgtctt gtaacgcttt acgctggcag
      301 ccaggcgtga ggcacgttgc ggtgcttccc agcggtcgtt actgtaatta gccacttcag
      361 ccagataatc cttttcaagt ttttcagccg tatagcacct gccgcctaaa aaaagccgca
      421 tggcctccct ggttttgtct ctcacatatc atccctcatt aatgctaatg tacgcataca
      481 ttagcattaa catgctgaca gcaacaagcg gcagatttac gtccgaataa cgtaaatgat
      541 tgcggtatac aggccaggcg gtcagatttc cagccgtgcc tgttcctgca gcagttccag
      601 atgatatgca gcaatagcgt ttaccacccc tgccgtcatc cgtgcgtcgg cgatcgctga
      661 gtgtgccagc ccttcccagg tcagtccggc ctggctggca gcactggcca gtgaaatcgt
      721 gccatagcgg ttggtggccc cataatatcc tgctgccagc tccatcgcac aatataccgt
      781 catttcttcc agccagtaag ccggatcgct atgtgcggca gcagtctgtt tcagaatgcg
      841 gat
//
LOCUS       DAZFKF010000346          823 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFKF010000346 DAZFKF010000000
VERSION     DAZFKF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.351"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            229..>823
                     /locus_tag="ACHKA1_005585"
     CDS             229..>823
                     /locus_tag="ACHKA1_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO5683075.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEAC"
BASE COUNT          239 a          189 c          182 g          213 t
ORIGIN      
        1 taattcagga ggcacagaat gcgcataaaa ataacagcat aaaacacctt accaccaccc
       61 aagagctctc tactatattg tttttcaatg aaaaaatatt attcacgcaa tatctcacca
      121 taaataacat taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg
      181 ataaatccta cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag
      241 caagtaacgg atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc
      301 actatcgcag aatcaaaacg ttttccgttg cacgaaatgc gcgacgatgt cgcatttcag
      361 attatcaatg acgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc
      421 cagacctggg acgacgaaaa tgtccacaag ttgatggatt tatccattaa caaaaactgg
      481 atcgacaaag aagaatatcc gcaatccgca gccatcgacc tgcgttgcgt aaatatggtt
      541 gccgatctgt ggcatgcgcc tgcgccgaaa aatggtcagg ccgttggcac caacaccatt
      601 ggttcttccg aggcctgtat gctcggcggg atggcgatga aatggcgttg gcgcaagcgt
      661 atggaagctg caggcaaacc aacggataaa ccaaacctgg tgtgcggtcc ggtacaaatc
      721 tgctggcata aattcgcccg ctactgggat gtggagctgc gtgagatccc tatgcgcccc
      781 ggtcagttgt ttatggaccc gaaacgcatg attgaagcct gcg
//
LOCUS       DAZFKF010000347          819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFKF010000347 DAZFKF010000000
VERSION     DAZFKF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.352"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..455)
                     /gene="yhaB"
                     /locus_tag="ACHKA1_005586"
     CDS             complement(<1..455)
                     /gene="yhaB"
                     /locus_tag="ACHKA1_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO5683076.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEKCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPA"
     gene            complement(711..>819)
                     /locus_tag="ACHKA1_005587"
     CDS             complement(711..>819)
                     /locus_tag="ACHKA1_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFO5683077.1"
                     /translation="YFKISNTGIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          238 a          154 c          158 g          269 t
ORIGIN      
        1 gcagggggtg ttaagatggc ttcttttcgg gtgaattttg cacaatactt agaaatcgtc
       61 tccttaatac attccagctc ttttttattg acggctgagc aagtacattt ttcgccaaaa
      121 ggtgcaaaag tgcctacatc taatgtttgt atgatataat cgtcatgttc tcctgtagaa
      181 atatctatga tgaatggact catattttcc ggttggattc ggatcatgac agaaggaaca
      241 tcgccttgat agaaattcaa tgcagtaatt gcagataata ctctccgcag ttcattactt
      301 atactgtata ttttattaac gtcattggtg gagacaattt ctgcaaactt tctttttacc
      361 gtccaataat caatatttct attgtgattg ttaaccactg cgtgcattgg tgggattgaa
      421 gggtgaaaaa tatgtgcaat tgggaagcct ttcattgatg cctgccttgt catcatgaca
      481 aacaataata gatgcttatt ttcctcgcag ggttctttcg cactatcaaa aaacgctcat
      541 ttacccggcg ttacagtaaa cgaagcaaca ttctgtggcc tacgccgatc aaaagctctg
      601 tgaaaatttc ccttgttgtc tttgtctgac cttatcgcgg cgatcagcaa ttattcgttt
      661 tgtgatcctc aacacaaaat gctattcgtc gcctatgaag ggagaagaat ttatggaaaa
      721 cgattgcatt cacaaaatga cgtcctgtca gaagagaaaa agttgacgtt aatcaattgt
      781 tcgagatgat caatacctgt attcgaaatt ttaaaatat
//
LOCUS       DAZFKF010000348          812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFKF010000348 DAZFKF010000000
VERSION     DAZFKF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.353"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            108..641
                     /locus_tag="ACHKA1_005588"
     CDS             108..641
                     /locus_tag="ACHKA1_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5683078.1"
                     /translation="MNTKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGNPVIPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRTRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            638..>812
                     /locus_tag="ACHKA1_005589"
     CDS             638..>812
                     /locus_tag="ACHKA1_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5683079.1"
                     /translation="MRRVTAIIYALVICIIVCLLWAVNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQ"
BASE COUNT          209 a          188 c          229 g          186 t
ORIGIN      
        1 atccgccctt taaaaaagtg ccggcttact ggcttcatcc cggactgact tatctgaaag
       61 tgcgtattta gtgggcctgg gacagcggct gaatatttaa tatatccatg aacacaaaaa
      121 taaaatacgg cctgtcggct gccgttctgg cgttgattgc cgcaggggct tctgcgcctg
      181 aaatcctcga ccagtttctg gatgaaaagg aaggcaacca caccacggca taccgtgatg
      241 gtgcgggtat ctggaccatc tgccgtggag ccacccgggt ggatggtaat cctgtgattc
      301 ctggcatgaa gctgtcgaag gaaaaatgcg accaggttaa cgccattgaa cgggataagg
      361 cgctggcatg ggtggagaaa aacatcagag tgccactgac tgaaccccag aaagcgggga
      421 tcgcgtcatt ctgtccttac aacattggcc cgggtaagtg tttcccgtcg acgttttata
      481 aacgaattaa tgcaggcgat cgcaggggag cgtgtgaagc gattcgctgg tggattaagg
      541 acggtgggcg tgattgccgc acacgttcaa ataactgcta cggacaggtt attcgtcgtg
      601 accaggaaag cgcattagct tgttggggga tagatcagtg agaagagtaa ccgcgattat
      661 ttacgctctg gttatctgca tcatcgtctg tctgttatgg gctgttaatc attaccgcga
      721 taacgccatc acctacaaag cgcagcgcga taaaaaagcc agagagctgg agctggcaaa
      781 cgcaaccatt actgatatgc aggtgcgcca gc
//
LOCUS       DAZFKF010000349          805 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFKF010000349 DAZFKF010000000
VERSION     DAZFKF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.354"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..246
                     /locus_tag="ACHKA1_005590"
     CDS             <1..246
                     /locus_tag="ACHKA1_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5683080.1"
                     /translation="ASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGN
                     PMPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          250 a          148 c          229 g          178 t
ORIGIN      
        1 gccagcatga tactgggtgg tgtggcccag atgctggccc cgaaggcaaa gacgccggag
       61 tacaaaagta cggataacgg taaacagaac acgtattttt cgtcactgga caacatgatt
      121 gcccagggta acccgatgcc ggtgccttac ggtgaaatgc tggttggttc acgacggata
      181 tcccaggaca tcagcacccg tgatgagggc ggagacggga aagtggtggt tatcgggcgg
      241 ggataaaaat aaaaaaaatc ccgcagagtt agcggagctg cgggaaataa cgatgaagat
      301 taacgttatg gagttatttt tttgggcaga aaaacattaa cgcagcgaaa ttataagcgc
      361 tacatggatt ttgcgaaaat gtgaagaaat tcagaaattt tttgctgcag aggtgcaggg
      421 agcttgccgg gtatttgtgg cagaagacag aaaacatcat gccggggtta agggataaaa
      481 aaatcccgca gagtccggag actgcgggac agaaccgatg aagattaacg ttcaaggagt
      541 tttgttttta ttgacccgaa aaaactgtaa cgccccggaa tgatatctgc cacggggacg
      601 tacagaaaat gtgaagaaat tcagactttt tctgtcgcag gtttacagaa cggatgaccg
      661 ccagggtacc agtggcgtaa atgagcagga tgttgtggtg tggctgaagc aaaaaaatct
      721 cccgtaatat cgccggatac tgacgggaga cgaatagtca ccttaaggag gtctgataat
      781 tattgctttt attgagcaaa aaaac
//
LOCUS       DAZFKF010000350          791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFKF010000350 DAZFKF010000000
VERSION     DAZFKF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.355"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>791)
                     /locus_tag="ACHKA1_005591"
     CDS             complement(<1..>791)
                     /locus_tag="ACHKA1_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683081.1"
                     /translation="SIGNDISSDLIRRKMNDLPESPTGNNLEALAPGIEKLKQTSIEM
                     VTLLNALQPGGKCVITGDFQKELAYLQNVILYNDSSLRMDFFGYNARIIQRSDNTCEL
                     TINEPLKNQEISTGNINVNCPLKDIYNEIRRLNVVFSCGTGGIVDLSSLDLRNVDLEL
                     YDFTDKHMANAILNPFKLDDTDFTNANMFQVNFVSSKQNTTISWDYLLKITPVLTSIS
                     DMYSEEKIKLVESCLNELGDITEEQLKIMRFAIIESIPRATLTDQ"
BASE COUNT          242 a          132 c          130 g          287 t
ORIGIN      
        1 aattgatctg ttagagttgc tcttggtata gattctataa ttgcaaatct catgattttt
       61 aattgttctt cggtaatatc tccaagctca tttaaacaac tttcgacaag cttaattttt
      121 tcttcagaat acatatcgct aatgcttgtt agaacaggcg ttatttttag taaataatcc
      181 caggagattg tggtgttttg ttttgaacta acgaaattta cctgaaacat gttggcatta
      241 gtaaaatctg tgtcatccaa tttaaaagga tttaaaattg cattggccat atgtttatct
      301 gtaaaatcat aaagttctaa atcgacattc cgtaagtcca gagaggatag atcaactata
      361 cctccagttc cacaactaaa aactacgttt aacctcctga tttcattgta aatatctttc
      421 aatggacaat taacattgat attgcctgtg gatatttcct ggttttttaa cggttcatta
      481 attgtaagtt cgcaggtatt gtctgatcgt tgaataattc gggcgttata accaaaaaaa
      541 tccatacgaa gagacgaatc attataaaga attacatttt gtaagtacgc taattctttt
      601 tgaaaatcac cagtgataac acatttccca cctggttgta acgcgttaag taaagtgacc
      661 atttcaatag aggtctgttt tagtttttct atacctggtg ctagtgcttc gaggttattt
      721 cctgttgggc tttcaggaag gtcattcatt tttcgacgga ttaaatcact actaatatca
      781 ttaccaattg a
//
LOCUS       DAZFKF010000351          772 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFKF010000351 DAZFKF010000000
VERSION     DAZFKF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.356"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            224..299
                     /locus_tag="ACHKA1_005592"
     tRNA            224..299
                     /locus_tag="ACHKA1_005592"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:257..259,aa:Ile,seq:cat)
BASE COUNT          228 a          141 c          175 g          228 t
ORIGIN      
        1 attacaaagt atcattgaat cgatgataac tatagatagt gttaattatt aacatttgct
       61 tgttgactgc gagtatcgac tttgcgaatg gaggcgatgt cgggttttat gtcgcatgta
      121 ttgacgaccc aggatggatg agaacgcata aaaatctgtt atttacatca taagatcatc
      181 atgtttattg cgtggtttat aagtattgtt attgcatact taaggccctt tagctcagtg
      241 gttagagcag gcgactcata atcgcttggt cgctggttca aatccagcag gggccaccaa
      301 atcatcattc atgtatcagg aagagttaag ggtacaaggg gtactcgatg gcggtctata
      361 tttcatgact aacgccattt aaaggaagaa gtgcggtggg tggtttcgaa tgagggctgg
      421 tgttttggta cgtggcacgc cactgcaatg tatttatcca tcagtactca ttcaaaagta
      481 cctggaaaaa atgcccgcta tgccaagcgg gcaaattgct tacatgtgga attaagtaat
      541 taacgataaa gccagagtat tatcatttat tatacccatt catttaatca ggttaattac
      601 tcaaatcact gaactattct ctgttcgctc actgatgatg agtgagtacc acaaccaatg
      661 tatgtagaac aatgccgtag agtaaggatg aaatgtctct gccacgcatt aaacatctac
      721 tactgaccag aacacccgcc gcaggtagat gagttttgtg agcgttacag ga
//
LOCUS       DAZFKF010000352          749 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFKF010000352 DAZFKF010000000
VERSION     DAZFKF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.357"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            147..743
                     /gene="fimE"
                     /locus_tag="ACHKA1_005593"
     CDS             147..743
                     /gene="fimE"
                     /locus_tag="ACHKA1_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO5683082.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          192 a          143 c          183 g          231 t
ORIGIN      
        1 tatttttgta tgatatttaa tgtaattgac tgatagccac atcacttcgt gtgtggttat
       61 ctttttatct attgggctaa ttttgaccga ttgaggtttc ctataggtat tcattcaaat
      121 atatctcagt taggagtact actattgtga gtaaacgtcg ttatcttacc ggtaaagaag
      181 ttcaggccat gatgcaggcg gtttgttacg gggcaacggg agccagagat tattgtctta
      241 ttctgttggc atatcggcat gggatgcgta ttagtgaact gcttgatctg cattatcagg
      301 atcttgacct taatgaaggt cgaataaata ttcgccgact gaagaacgga ttttctaccg
      361 ttcacccgtt acgttttgat gagcgtgaag ctgtggaacg ctggaccctg gaacgtgcta
      421 actggaaagg cgctgaccgg accgacgcca tatttatttc tcgccgcggg agtcggcttt
      481 ctcgccagca ggcctatcgc attattcgtg atgccggtat tgaagctgga accgtaacgc
      541 agactcatcc tcatatgtta aggcatgctt gcggttatga actggcggag cgtggtgcag
      601 atactcgttt aattcaggat tatctcgggc atcgaaatat tcgccatact gtacgttata
      661 ccgccagtaa tgctgctcgt tttgccggat tatgggaaag aaataatctc ataaacgaaa
      721 aattaaaaag agaagaggtt tgatttaac
//
LOCUS       DAZFKF010000353          739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFKF010000353 DAZFKF010000000
VERSION     DAZFKF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.358"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..92
                     /gene="yjbL"
                     /locus_tag="ACHKA1_005594"
     CDS             <1..92
                     /gene="yjbL"
                     /locus_tag="ACHKA1_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFO5683083.1"
                     /translation="HRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            83..>739
                     /locus_tag="ACHKA1_005595"
     CDS             83..>739
                     /locus_tag="ACHKA1_005595"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO5683084.1"
                     /translation="MFLIEQITNLKEWVNKYIGDCTDEELNDRDFIASVVDRAIFHFA
                     INSICNPEDNKDATPIERYTFDVETKNGLPSTVQLFYEESKDNEPLANIHLQAIGSGF
                     LTFVNACQKYDDNSLKLFASLLISLSYSSAYTDLAGAEKVNINDDNENYLTAQFEELS
                     QRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGKLAPDILSKMRDAAPKLIDFAK
                     S"
BASE COUNT          248 a          109 c          153 g          229 t
ORIGIN      
        1 gtcatcggga tataattatt gcaaaaatag atttgtttat tcaaagaatc actcataatt
       61 tatggcatgc acgtaaaaaa atgtgttttt aattgagcaa ataactaatc tcaaagagtg
      121 ggttaataag tatattggag attgcactga tgaggaatta aacgatcgtg actttattgc
      181 atcagttgtt gaccgggcta tttttcattt cgcgattaat agtatatgta atcctgagga
      241 taataaagat gcaacgccca tagaacgata tacttttgat gtagaaacca agaatggcct
      301 tccctccacg gttcagttat tttatgaaga atctaaagat aatgaacctt tagcaaatat
      361 acatttacaa gcaataggtt ctggtttttt aacgtttgtt aatgcatgtc agaaatatga
      421 tgacaacagc ttaaaattat tcgcttcgct gttgatttca ctttcatact ctagtgccta
      481 cacagattta gcaggagcag aaaaagtgaa tattaatgat gataatgaga actatctgac
      541 agctcagttt gaagagttat ctcaacgtga tatgaagaag tacctgggag agatgaagcg
      601 tctggcagat gggggagaaa tgaattttga tggctatctg gataagatgt cacatctggt
      661 gaatgaagga aagctcgccc ctgatatttt aagcaaaatg cgagatgctg caccgaaatt
      721 aattgacttt gctaagtcg
//
LOCUS       DAZFKF010000354          732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFKF010000354 DAZFKF010000000
VERSION     DAZFKF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.359"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     repeat_region   196..590
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=196..224
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT          190 a          163 c          184 g          195 t
ORIGIN      
        1 ttcgctggag tgaaaattat tgcggtaaag tttggtagat tttagtttgt atagagttat
       61 tttaaatatt taccttttta atcaattggt taagtgctct ttaacataat ggatgtgttg
      121 tttgtgtgat actgtaaagt tggtagattg tgactggctt aaaaaatcat taattaataa
      181 taggttatgt ttagagagtt ccccgcgcca gcggggataa accgggcgct tcggtgtcga
      241 tctgggcgtt gatggcgagt tccccgcgcc agcggggata aaccgatgtc ggctgcaatc
      301 tgccgcgccg ggcgtgtgag ttccccgcgc cagcggggat aaaccggctg gatacagtct
      361 ggcagggttc tgtgatgaga gttccccgcg ccagcgggga taaaccgatg atgcagccgt
      421 ctcaggacga ccttcgcacg agttccccgc gccagcgggg ataaaccgaa cggctacggc
      481 actgaggtga caaaccagat gagttccccg cgccagcggg gataaaccgg tttctggcat
      541 cggaaaacat tgcatatcgt cgagttcccc gcgccagcgg ggataaacca ccatataacc
      601 cgttatctct ttctcaagtt tttatattag ctgtacttgt aataagcaac atatccacat
      661 aacacctcat gttcaaaata gttccccatg ccagataagt tcacaattat cgatacaaaa
      721 aatcaaattt aa
//
LOCUS       DAZFKF010000355          731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFKF010000355 DAZFKF010000000
VERSION     DAZFKF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.360"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..624
                     /locus_tag="ACHKA1_005596"
     CDS             <1..624
                     /locus_tag="ACHKA1_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFO5683085.1"
                     /translation="EVRREIRDSDLRQLAATLNRDLVYPLYALNTAHAIDIRRLPRIC
                     FQTKEPGDITKITSAVMQLSTGMDISDPWIREQTGIPQPTPGEAIFRVRQSGNEPAQT
                     DKEMPPEKQEKTEQTALAARLPEAKSSPRDELDDMGDAVPARRLQEAIDPVLEPVIDA
                     IRTRGLAEALADLPALYREMDDSRLMTLLSDAMFAAEMKGMLDGTGD"
     gene            608..>731
                     /locus_tag="ACHKA1_005597"
     CDS             608..>731
                     /locus_tag="ACHKA1_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683086.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGVHISWNWFETDADVH"
BASE COUNT          170 a          200 c          226 g          135 t
ORIGIN      
        1 gaggtgcgcc gggaaatcag ggattctgat ttacgccagc tggccgccac gctgaaccgc
       61 gatctggtgt atccgctgta tgccctgaac accgcccacg ccattgatat ccgtcgcctg
      121 ccgcgtatct gtttccagac aaaagagccg ggggatatca ccaaaatcac cagtgcggtg
      181 atgcagctca gtacggggat ggatatttct gatccctgga taagggagca gaccggtatt
      241 ccgcagccca cgcccggtga agccatcttc cgtgtccgtc agagtggcaa tgaacctgct
      301 cagacagaca aagagatgcc accggaaaaa caggagaaga cagagcagac agcgctggct
      361 gccagactgc cggaagcgaa aagcagcccc cgtgatgaac tggacgacat gggggatgcg
      421 gtgcctgccc gccggttaca ggaggccatc gacccggtac tggagccggt cattgatgcc
      481 atcagaacgc gggggctggc ggaggcactg gcagacctgc ccgcccttta ccgtgaaatg
      541 gatgattccc gcctgatgac gctgctcagt gatgccatgt ttgctgcgga aatgaagggg
      601 atgctggatg gcacagggga ttgatttagg ttatgccgcc acccttccct caaaagaggc
      661 ggtggcatac ttccgcgcca aaggggtgca tatcagctgg aactggttcg aaacagacgc
      721 ggatgttcat g
//
LOCUS       DAZFKF010000356          727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFKF010000356 DAZFKF010000000
VERSION     DAZFKF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.361"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..268
                     /locus_tag="ACHKA1_005598"
     CDS             <1..268
                     /locus_tag="ACHKA1_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683087.1"
                     /translation="LNNKDNKDKKDVARINEIKEILKTIDGKLPNISDEILHLKDETE
                     RLEEEYQQENTLNDVVQNIRRWLKENENMVKAIKKIDTNNMANK"
BASE COUNT          268 a          113 c          151 g          195 t
ORIGIN      
        1 attaaataat aaagacaata aagataaaaa agatgtagcc agaattaatg agattaagga
       61 aatactaaag acaatagatg gaaaactacc aaatataagt gatgagattc tacatcttaa
      121 ggatgaaaca gagagattgg aggaagagta tcaacaagaa aatacactta atgatgtagt
      181 tcaaaatatc aggcgttggc taaaagaaaa tgaaaatatg gtgaaagcta ttaagaaaat
      241 tgatactaat aacatggcga ataaataaac ggatcaagtc gttcctgtga caatgaggtt
      301 atacgaaaaa gacgcttacg cattagaaat ctggtgtact ttaattaaac cagtgtcgct
      361 gatgtgtaag cgtcgacgtt gggagacgat taaattgtta tatcaccgct acgccctttc
      421 cagacggcgt ataagagtgc aatatgttga gttttccgag tgaatggtgc attttattaa
      481 ccgagccatt tgaataagcc aacgcaataa aacagactgg aataataagt ctgtatttat
      541 tgttcatcac aatcgcactc tacggtttta tcctgctgaa atgagacagc ctccagcgtt
      601 tgtggatgat ccttgaacga gagtcatggt ctggtgtcag gattcacaaa tgacctgttt
      661 cctgacatgg atataaaccc aacatagagt gcggtagtta atcttaaaaa tctatagtcg
      721 gctactc
//
LOCUS       DAZFKF010000357          727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFKF010000357 DAZFKF010000000
VERSION     DAZFKF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.362"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..178)
                     /locus_tag="ACHKA1_005599"
     CDS             complement(<1..178)
                     /locus_tag="ACHKA1_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO5683088.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAE"
     gene            complement(195..>727)
                     /locus_tag="ACHKA1_005600"
     CDS             complement(195..>727)
                     /locus_tag="ACHKA1_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tlde1 domain-containing protein"
                     /protein_id="HFO5683089.1"
                     /translation="YITQDPIGLKGGWNFYQYPLNPITDIDPLGLATCLYSITLSMLS
                     CVSDTQNDDNSYDVLTIPVASGNNGNNMQCKNNPGCTHLQNRGPIPQGVWSWNVNGPG
                     ATNRKPNGIRLVPSANTETYNRDGFLTHSCLNAFGPSLGPRFCSEGCITGSSNDMQKL
                     NELIFSEPDSTLTVTD"
BASE COUNT          221 a          153 c          123 g          230 t
ORIGIN      
        1 gttcagcctc tgcagcctta caggcaaaat agctaagtga acacgctttt cgtccctctt
       61 tagtactggc tgatattagt gcccataata ttgctttgtc tgccaaatct aactctctct
      121 gaaattgtct tgcagtaact gcactcgcag aaatagagaa aaaaatacaa ttgttaataa
      181 ccttgccata ttttttagtc cgtaactgtt aatgtgctat ctggctctga aaatattaac
      241 tcatttaatt tttgcatgtc attagatgaa ccagttatac aaccctcgga acagaatcga
      301 ggtcctaaag acggaccgaa agcgtttaaa caactatgtg ttaaaaatcc atcacgatta
      361 tatgtttctg tatttgctga cggtactaaa cgtataccat taggttttct attggttgct
      421 ccaggaccat taacattcca gctccatacc ccttgaggta ttggacctct attttgtaag
      481 tgtgtacaac caggattatt tttacattgc atattattac cattattacc agaggcgaca
      541 ggaatagtaa gcacatcata agagttatca tcattttgcg tatctgaaac gcatgaaagc
      601 atacttaaag ttatgctgta taaacaggtc gctaatccca acgggtcaat atcagttatc
      661 ggattcaacg gatactgata aaaattccat ccccctttca gcccaatcgg atcctgagtg
      721 atatatc
//
LOCUS       DAZFKF010000358          723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFKF010000358 DAZFKF010000000
VERSION     DAZFKF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.363"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..649
                     /locus_tag="ACHKA1_005601"
     CDS             <1..649
                     /locus_tag="ACHKA1_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5683090.1"
                     /translation="AKQDQGYKNHYLSDEMLAGKKELYEFTPESIYRAMTIFDGLQNK
                     SDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSNITNVDGKILAIMG
                     LITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVNFHNEKIEEARICIDKSLQLEP
                     RRRKAVVIKECVDMYVPNPLKNNMKLYYKETESESHRVIIDNILKLKQLTRICMR"
BASE COUNT          273 a          118 c          131 g          201 t
ORIGIN      
        1 agctaagcag gaccaaggat ataagaatca ttatttatca gatgaaatgt tagccggaaa
       61 gaaagaactt tacgagttca cccctgaaag catttacagg gccatgacta tatttgatgg
      121 attacaaaat aaaagtgata tacagacgct aaaaacagaa tgttattgcc ttctagcgga
      181 atgccatatg tctttggcac ttcatggaaa aagtgaactt gaacttgctg ctcaaaaagc
      241 attagagctt ttagattatg tatcaaacat aaccaatgtc gatggaaaaa ttttagctat
      301 tatgggactg ataactggcc tgtctggaca agcaaaagta tctcatatct tatttgaaca
      361 ggctaagata cactcaactg atatagcctc tctctactac tatagggcac ttgtcaactt
      421 tcataatgaa aaaattgaag aggcaaggat ttgtatagac aaatcactac aactcgaacc
      481 cagaagacga aaagcagttg tgataaaaga atgtgtagat atgtatgtgc ctaacccgct
      541 caaaaacaac atgaaactct actataaaga aactgagagt gaaagccatc gagttataat
      601 tgacaacatt ttgaaactaa agcagctgac gagaatttgt atgcgataaa tttttattga
      661 cgcaatttta ttgtttagat gtatgcaact tgttatttaa acaacagaga taaaaatatc
      721 taa
//
LOCUS       DAZFKF010000359          720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFKF010000359 DAZFKF010000000
VERSION     DAZFKF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.364"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..720
                     /locus_tag="ACHKA1_005602"
                     /pseudo
     CDS             1..720
                     /locus_tag="ACHKA1_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5503 domain-containing protein"
BASE COUNT          236 a          126 c          132 g          226 t
ORIGIN      
        1 caaattattt aatatgctgt cagaatttaa gttattgcac tcagtatatt tcgaatgggg
       61 tgattatagt ctctggtttc aagatttctc gatttataat aagataggtt ttatcatgat
      121 agaaaaaaat cagggaactg gtaacccacc cattcggcat aaattagagt ttatatcgac
      181 taatattgca gagtttctag acaactttac gaaaattaca gattccagac tttgcaaagg
      241 tttcagtgac tgggctaatt cagtcaaaga aggcgcatcg aatgacttca aaaaaaatgt
      301 ggatatagca ttggtgagat tgtttaaatg tgttgaactc cacaatagta aacttgactt
      361 aacagatctt cacttggggt ctttgccacc tcttccatcc tggattgagg tgctttatct
      421 tcgtcataat ggccttgcca caatacaggt tcccaaattc tgcaaagaat tagagctcga
      481 tttcaataat tatatggtat ttccaaaagt atccgatgga attacccagg tctccgtaga
      541 taataatctg atatcgcgtg ttgactcatc tccgtcaaag gctatgacaa tttctattta
      601 tcgcaataaa atatggtaaa taccagcaat gcctgagact actaagctgt ttaattgtgg
      661 cgataataag ataaaagaaa ttcaatactt cccaaatcat ctggagaaag cacgtatgga
//
LOCUS       DAZFKF010000360          684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFKF010000360 DAZFKF010000000
VERSION     DAZFKF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.365"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..602)
                     /locus_tag="ACHKA1_005603"
     CDS             complement(<1..602)
                     /locus_tag="ACHKA1_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO5683091.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDE"
BASE COUNT          167 a          170 c          164 g          183 t
ORIGIN      
        1 ttttcgtcgc aggcttcaat catgcgtttc ggatccataa acaactgacc ggggcgcata
       61 gggatctcac gcagctccac atcccagtag cgggcgaatt tatgccagca gatttgtacc
      121 ggaccgcaca ccaggtttgg tttatccgtt ggtttgcctg cagcttccat acgcttgcgc
      181 caacgccatt tcatcgccat cccgccgagc atacaggcct cggaagaacc aatggtgttg
      241 gtgccaacgg cctgaccatt tttcggcgca ggcgcatgcc acagatcggc aaccatgttt
      301 acgcaacgca ggtcgatggc tgcggattgc ggatattctt ctttgtcgat ccagttttta
      361 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
      421 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      481 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      541 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      601 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      661 agcaaggcat tacaggctta attt
//
LOCUS       DAZFKF010000361          677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFKF010000361 DAZFKF010000000
VERSION     DAZFKF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.366"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..672
                     /locus_tag="ACHKA1_005604"
     rRNA            <1..672
                     /locus_tag="ACHKA1_005604"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          159 a          151 c          222 g          145 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gttcggtccc tatctgccgt gggcgctgga gaactgaggg gggctgctcc
      421 tagtacgaga ggaccggagt ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac
      481 tgcccggtag ctaaatgcgg aagagataag tgctgaaagc atctaagcac gaaacttgcc
      541 ccgagatgag ttctccctga ctccttgaga gtcctgaagg aacgttgaag acgacgacgt
      601 tgataggccg ggtgtgtaag cgcagcgatg cgttgagcta accggtacta atgaaccgtg
      661 aggcttaacc ttacaac
//
LOCUS       DAZFKF010000362          671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFKF010000362 DAZFKF010000000
VERSION     DAZFKF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.367"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            232..456
                     /locus_tag="ACHKA1_005605"
     CDS             232..456
                     /locus_tag="ACHKA1_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683092.1"
                     /translation="MKQSTFPVIVSTTGHVFSVVRVTLCTICLKHEKTGEAYVVIFTD
                     CHNIRDYKKGVVPVLGELYQEDVDLITGKS"
BASE COUNT          220 a          103 c          146 g          202 t
ORIGIN      
        1 acaggagcat tcagtttaca tgtgaaatat cagcaagtat cgaaattatg gaggatgatt
       61 tcgtaaataa agctggtata ttaaagatgt tagatgaatt tattgaaaat gatttttcaa
      121 taaagctcgt ttagaaaata cttgataata ggtcatttat gacctattat taatgcattg
      181 agaggggatg gtccccaatc gccaaagccg ctcagcggca ggagattaaa tatgaaacaa
      241 tctacttttc ctgttatcgt ctctacaact ggtcatgtgt tttctgttgt gcgcgtgacg
      301 ctttgcacaa tttgccttaa acatgaaaaa acaggcgagg cttatgtcgt catttttaca
      361 gactgccata atatccgtga ttataaaaag ggcgttgttc cggttcttgg ggagctttat
      421 caggaagatg tcgatttgat taccggaaaa agttaaaaga aaggggctat atgccccttt
      481 actgttaaag gggagactat ctcaatatga agataagaaa aggtgacaga caatattatc
      541 tgaataaaga aggtgataca tttcatctgg tgaagcgagt aaaaactttt tctaaatctg
      601 cgactttagg aaaaacgaaa gcaacggtga aaactgttgc cgatcttgtt tttcatgaga
      661 aggcattcga c
//
LOCUS       DAZFKF010000363          670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFKF010000363 DAZFKF010000000
VERSION     DAZFKF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.368"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(89..547)
                     /gene="tnpA"
                     /locus_tag="ACHKA1_005606"
     CDS             complement(89..547)
                     /gene="tnpA"
                     /locus_tag="ACHKA1_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HFO5683093.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
BASE COUNT          155 a          187 c          126 g          202 t
ORIGIN      
        1 ccggaggttt ttcaaatgcg cctataaggc tctcttacca gccgcgccct aacaggcgca
       61 cacgatctga catttgcatc caacttcgtt acttacggcc cgtaaacggg ctgcccggat
      121 agggaatcga taactgctct cccattttat cctcttcaag ctggtgcttt atgtaatcct
      181 gtatcttcgc cgtgttctta cccaccgtat cgacgtagta ccctctgcac cagaactctc
      241 tgttcctgta tttgaatttc aaatcaccaa actgctcgta aagcatcaga ctgcttttcc
      301 ctttcagata tcccataaag ccggatacgc tcattttggg cgggatctcc acaagcatat
      361 ggatatgatc tgcacagcat tcagcttcaa gaatccgtac gcttttccac tcgcacagct
      421 ttctcaaaat actgcctatt gctctacgtt tctctctgta gaacacctgt cttcggtatt
      481 ttggcgcaaa aactatgtga tatttacagt tccatcgggt gtgcgctaag ctcttttcgt
      541 tccccattgg gacccccttt tgatttcttg tttgacactt gcagttgcca gaccgcaagg
      601 tgttttaaca aatcaaaagg ggttttaata actggctcaa agctgaaagc tttccggaac
      661 ccccagccta
//
LOCUS       DAZFKF010000364          670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFKF010000364 DAZFKF010000000
VERSION     DAZFKF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.369"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..531
                     /locus_tag="ACHKA1_005607"
     CDS             <1..531
                     /locus_tag="ACHKA1_005607"
                     /inference="COORDINATES: protein motif:HMM:NF018510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5683094.1"
                     /translation="AAEAIRALSLQVPGFRQKLYEGWYQLRIAGRDVSEQELHSRVCE
                     PLNDGDVIHLVPRTEGAKNGGVLQVVAGAVLAVVGYVFSWTGIGAVIGNLGVAMMVGG
                     ITQMLTPRAKTPSATTTDNGKRNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDIS
                     TRDEGGDGKVVVIGRG"
BASE COUNT          164 a          138 c          219 g          149 t
ORIGIN      
        1 gcagcggagg ccatccgtgc cctgtcgctg caggtgccgg gattccggca gaaactgtat
       61 gagggatggt atcagttacg cattgcgggc agggatgtat ctgaacagga gcttcattcc
      121 agagtatgtg agccgttgaa tgacggggat gttatccatc tggtgccccg gacggagggg
      181 gcaaaaaacg gcggtgttct tcaggtggtg gccggtgctg ttctggctgt tgtgggatat
      241 gtgttcagct ggaccggtat tggtgctgtc atcggtaatc ttggggtggc catgatggtg
      301 gggggtatca cgcagatgct cacccccaga gcgaaaacac cgtctgccac gacaacagat
      361 aatggtaaac ggaacacgta tttttcgtca ctggacaaca tgattgccca gggtaacccg
      421 atgccggtgc cttacggtga aatgctggtt ggttcacgac ggatatccca ggacatcagc
      481 acccgtgatg agggcggaga cgggaaagtg gtggttatcg ggcggggata aaaataaaaa
      541 aatcccgcag agttagcgga gctgcgggag agaacgatga agattaacgt tatggagtta
      601 tttttcaggc atcaaaaaag taacgcagcg tcattattgc ggctacaggc aattgccgga
      661 aatgtgaaga
//
LOCUS       DAZFKF010000365          665 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFKF010000365 DAZFKF010000000
VERSION     DAZFKF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.370"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..558
                     /gene="ydeO"
                     /locus_tag="ACHKA1_005608"
     CDS             <1..558
                     /gene="ydeO"
                     /locus_tag="ACHKA1_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO5683095.1"
                     /translation="TVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLF
                     SCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKK
                     LKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPK
                     RVSKEYRCQRHTGMNTDNTMSALAI"
BASE COUNT          201 a          132 c          138 g          194 t
ORIGIN      
        1 acagtgacgg tgcctgttcc tgcgaagttt ttacgtgctt caaatgtacc gactggattg
       61 cttaatgaaa tgattgctta tctcaactcg gaagaacgca atcatcataa tttttcagaa
      121 cttttgcttt tttcttgcct gtctattttt gccgcatgca aaggtttcat tacactatta
      181 actaacggtg tgctatccgt ttccgggaaa gtgagaaata ttgtcaacat gaagctggcg
      241 cacccatgga agctgaaaga tatttgtgac tgcctgtaca tcagtgaaag cctgttgaag
      301 aaaaaactaa agcaagagca aacgacattc tcacagattc tattagatgc cagaatgcag
      361 cacgcaaaaa atttgatacg cgtagaaggt tcagtcaata aaattgccga acaatgtggt
      421 tatgccagta catcttattt tatttatgcg ttccgcaaac atttcggcaa cagtccgaag
      481 agagtttcta aggagtaccg ttgtcaaagg cacaccggta tgaacacgga caacacgatg
      541 agtgctttag ctatttgatt atttgctaac gggtagtcaa ccacacacgc tacgtaagaa
      601 ttaaatgggg cagccattcc ttgccccgcg ttgtttttag gcgatatatt tattgaaata
      661 aataa
//
LOCUS       DAZFKF010000366          665 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFKF010000366 DAZFKF010000000
VERSION     DAZFKF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.371"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>665
                     /locus_tag="ACHKA1_005609"
     CDS             <1..>665
                     /locus_tag="ACHKA1_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO5683096.1"
                     /translation="QYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSNDK
                     DVIASDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPELLNM
                     LDIKGKIITIDAIGCQKDIAEKIQKQGGDYLFAVKENQGRLNKAFEEKFPLKELNNSK
                     HDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEP
                     EMTV"
BASE COUNT          223 a          116 c          151 g          175 t
ORIGIN      
        1 caatatggtg attttgaaaa tggtattcct gttcacgata ccattgccag agttgtatcc
       61 tgtatcagtc ctgcaaaatt tcacgagtgc tttattaact ggatgcgtga ctgccattca
      121 tcaaatgata aagacgtcat tgcaagtgat ggaaaaacgc tccggcattc ttatgacaag
      181 agtcgccgca ggggagcgat tcatgtcatt agtgcgttct caacaatgca cagtctggtc
      241 atcggacaga tcaggacaga tgagaaatct aatgagatca cagctatccc tgaacttctt
      301 aacatgttgg atattaaagg aaaaattatc acaattgatg cgattggttg ccagaaagat
      361 attgcagaga agatacaaaa acagggaggt gattatttat tcgctgtaaa agaaaaccag
      421 gggcggctaa ataaagcctt tgaggaaaaa tttccactga aagaattaaa taattcaaag
      481 catgacagtt acgcaattag tgaaaagagt cacggcagag aagagatccg tcttcatatt
      541 gtttgcgatg tccctgatga acttattgat ttcacgtttg aatggaaagg gctgaagaaa
      601 ttatgcgtgg cagtctcctt tcggtccata atagcagaac aaaagaaaga gccagaaatg
      661 acggt
//
LOCUS       DAZFKF010000367          652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFKF010000367 DAZFKF010000000
VERSION     DAZFKF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.372"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..256
                     /locus_tag="ACHKA1_005610"
     CDS             <1..256
                     /locus_tag="ACHKA1_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683097.1"
                     /translation="SFSSPSGLDNQGNIFVGHAKYVNEMDFTINSFSQKMYVAMVKNV
                     DNEVKLQCARYITTGSVDEQNSISDKIAFTFYGAATTADL"
     gene            471..>652
                     /locus_tag="ACHKA1_005611"
     CDS             471..>652
                     /locus_tag="ACHKA1_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683098.1"
                     /translation="MLTIDASNNVKICKVSYCEVTMVSVTSYTYPTETENDNSQFQSQ
                     SLRESIDKSLEDNLHK"
BASE COUNT          216 a           91 c          134 g          211 t
ORIGIN      
        1 aagttttagt tcacccagtg gtttggacaa ccagggaaat atttttgtgg gtcatgctaa
       61 atatgttaat gaaatggatt ttactataaa tagttttagc caaaagatgt atgtagcgat
      121 ggtgaaaaac gttgataatg aagttaagct tcagtgcgct cgttatatta ctaccggtag
      181 tgtcgacgaa caaaactcca tcagcgataa aatcgctttt acattttacg gtgccgcaac
      241 aactgctgat ttatgatttt tgcctgataa atcgcgttta tcattaagtt gatcgcgtat
      301 tcttaaccgt ctgtgtgtaa acagtctttt taaaaaaatg agcgattttt gatagtgaat
      361 aagtagtgtt agtgatgcaa ttgcattggg gaaatcgata aaaattaact gcactatagt
      421 cttattattg aaataaatga aatatagcgg ttagtattga gatatttatc atgctaacca
      481 ttgatgcttc taacaatgtc aaaatatgca aagtgtcata ttgcgaggtt actatggtta
      541 gtgtgacgag ttatacatat ccaacggaaa cagagaatga taatagccag tttcagagtc
      601 agagtctaag ggagagtatc gacaaaagtc tcgaggataa tcttcataaa tt
//
LOCUS       DAZFKF010000368          649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFKF010000368 DAZFKF010000000
VERSION     DAZFKF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.373"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            39..134
                     /locus_tag="ACHKA1_005612"
     CDS             39..134
                     /locus_tag="ACHKA1_005612"
                     /inference="COORDINATES: protein motif:HMM:NF026967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HFO5683099.1"
                     /translation="MEGKQPKTLGGNFTILIAKKDGQVLNVIHTK"
BASE COUNT          216 a          127 c          134 g          172 t
ORIGIN      
        1 aaccatatag tattacagat gacaataatt gttggaaaat cgagggaaaa caaccgaaga
       61 ctttaggagg aaattttaca atactaatag cgaaaaaaga tgggcaagtg ttgaatgtaa
      121 tacacacaaa ataatccact gtacagtcga ttcaaatatt ttaccaaggt gtgtttcgta
      181 acaattttgt gtacaatatg actctacata tcgacgaaaa aaccacttga tttgacagga
      241 acacattcaa aaaaatttct tttttcattt caggagcgat tttcatctgt tacttgaagt
      301 atctgtgaga ttaagtgact ccccctaacg tcgtactaaa tagtaattac tggccatacc
      361 gggagcagta ttcggcgctc cccacatgca gcagaataac cgcatcagcg aagatgtatt
      421 gtatttttac tatcacaatg tgtacggacg gtttatggaa aagaatgaac attagatacg
      481 cgatgcggcg gcaggttaca cggttatcac tacgatagcc aatactggtt ggtgcattac
      541 cgccgtgaac aataggacgc cgtactgctg gaaatttctt acctttatga ctccccgcgc
      601 cgccgtatcg ggaaacggga agcaatcaca gggaaagatc atcagaagt
//
LOCUS       DAZFKF010000369          643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFKF010000369 DAZFKF010000000
VERSION     DAZFKF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.374"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..590
                     /locus_tag="ACHKA1_005613"
     CDS             <1..590
                     /locus_tag="ACHKA1_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFO5683100.1"
                     /translation="YITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTN
                     GLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPN
                     RGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSF
                     YLGTPSGNFKAIDNHGKEITNGKGLPNVCRVHGNM"
     gene            562..643
                     /locus_tag="ACHKA1_005614"
                     /pseudo
     CDS             562..643
                     /locus_tag="ACHKA1_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          221 a          100 c          141 g          181 t
ORIGIN      
        1 ggtatatcac tcaggatccg attgggctga aggggggatg gaacctgtat ggatatcaat
       61 tgaatccgat atcagacatc gaccccctgg gtttatctat gtgggaggat gcaaaatcgg
      121 gggcatgtac taatggtctt tgcggcacac tatccgctat gataggtcca gataaatttg
      181 attctataga tagcaccgca tatgacgcct taaataaaat aaatagccaa tctatttgcg
      241 aagataaaga gttcgctggt ttaatatgta aggataatag tggcagatat ttctcaacag
      301 cacctaaccg aggagaaaga aaaggatcat atccattcaa tagcccttgc cctaatggta
      361 ctgagaaagt atcagcttat catactcatg gtgcagatag tcatggagaa tattgggacg
      421 aaatattttc aggtaaagat gagaaaatag ttaaaagtaa agataacaat atcaagtcat
      481 tttatttagg tacgcccagt ggtaatttta aagcaataga taaccacggg aaggaaataa
      541 caaacggaaa aggattacct aatgtctgca gagttcatgg taatatgtaa aaaatattgt
      601 ttaggaactg tgtcattgta tctttgtttg tttttacata caa
//
LOCUS       DAZFKF010000370          636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFKF010000370 DAZFKF010000000
VERSION     DAZFKF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.375"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..123
                     /locus_tag="ACHKA1_005615"
     CDS             <1..123
                     /locus_tag="ACHKA1_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683101.1"
                     /translation="CTQLHSRTFQVNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT          151 a          145 c          177 g          163 t
ORIGIN      
        1 tgtacgcaat tacactcccg gacctttcag gtgaatgcca cgaatgaaga gctaaccgtt
       61 gagtgtacga tgaatgcagc taaatgccgc atggtatcgg ctgcattaca gccagtgtgt
      121 tgagcgacct tattatccat gcgcggtatt gtcgccgtat tcctgcatta acagagaccg
      181 cagcccgaca gggagactcc tctgcgcgag tgtgcgggga taatcaaaaa cgatacacac
      241 cggggtttac cgcgttaacg gagcgcggag ttgtcccctc atggtcgctg gtccggtgcg
      301 atggtggaag aaactggatt ttgttgcaac tgataaccat tatcatttta gcgggtcctt
      361 tccggcgatc cgggccgtta cggggcggcg acctcgcgcg ttttcactat ttatgaaaat
      421 tttccgggat ccatgtccgg tttctctgca agttaactat atgaaaaata taaaaacagg
      481 tcttctgtga accggacatg cgcaaaaaat gggcatgtga accggacatg acctgttttg
      541 ttgtgattgt gaggtgagag tttttgcgag gtgaggagtg gctacgcaga ctgaagttgc
      601 caggcattta agtctgaccg atcgccagct tcgcag
//
LOCUS       DAZFKF010000371          605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFKF010000371 DAZFKF010000000
VERSION     DAZFKF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.377"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>605
                     /locus_tag="ACHKA1_005616"
     CDS             <1..>605
                     /locus_tag="ACHKA1_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683102.1"
                     /translation="FFNVDEWKEKISTEYKQYCQKMISLLPDGAMNYRYVQVPYEKNN
                     SKHSCDLISDIISEINKIDSKLILIEAPAGFGKTCTSYEILNSLISIDGAPIPFFTEF
                     SRDRQAKVFSHVFVREVDRSFRTVKSDLVIDETKNGKIVLVLDGFDELLNDTTSNKTN
                     DIDFEKAQPMLETIGELLTKSAKVILTSRRAAIFDGEIFQE"
BASE COUNT          216 a          102 c          106 g          181 t
ORIGIN      
        1 gtttttttaa cgtagatgaa tggaaagaga aaatatcaac agaatacaag caatattgcc
       61 aaaaaatgat ttcattgcta cctgacggag caatgaacta tcgttatgta caagtgcctt
      121 atgagaaaaa caattcaaaa cattcttgtg acttaatctc tgatatcata tcagagatta
      181 acaaaatcga ttcaaaatta atccttattg aagctcccgc gggattcggc aaaacttgta
      241 catcctatga gatattgaat tcccttatat caattgatgg tgctcccatc ccatttttta
      301 ctgaattttc tcgcgataga caagcgaaag tttttagcca cgttttcgtt cgcgaagtag
      361 atcgaagttt ccgaacagtt aaaagtgatc tcgtaataga cgaaaccaaa aatgggaaaa
      421 tagttttagt acttgatggt tttgacgagt tgctaaatga tacaacatca aataaaacaa
      481 acgatattga ttttgaaaaa gctcaaccaa tgctagaaac aattggtgaa ttattaacaa
      541 agtctgcaaa agtaatcctc acgtctcgta gagctgctat ctttgatggt gagatatttc
      601 aagag
//
LOCUS       DAZFKF010000372          593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFKF010000372 DAZFKF010000000
VERSION     DAZFKF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.378"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>593
                     /gene="tssI"
                     /locus_tag="ACHKA1_005617"
     CDS             <1..>593
                     /gene="tssI"
                     /locus_tag="ACHKA1_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO5683103.1"
                     /translation="PGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQME
                     GWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGRRG
                     AGTALENHFAVIPADRTWRPQPRLKPLVDGPQSAVVTGPEGEEIFCDEHGRVRVKFNW
                     DRYNPADQDSSCWIRVAQAWAGTGFGNLAIPRVGQEV"
BASE COUNT          125 a          153 c          226 g           89 t
ORIGIN      
        1 cccggctggg caggacgttt tgatcaggaa ggccagcacc aggattacca gcgcacgcag
       61 tatgaggtgt atgactaccc cggacgtttc aagggcgccc acgggcagaa ctttgcccgc
      121 tggcagatgg agggctggcg aaacaatgca gaaaccgcgc ggggaatgag tcgctcgccg
      181 gagatatggc cgggacggcg aattgtgctg acggggcatc cgcaggcgaa cctgaaccgg
      241 gaatggcagg tggtggcaag tgaactgcac ggcgagcagc cgcaggcggt gccgggaagg
      301 cggggagcag ggacggcgct ggagaaccat tttgcggtga tcccggcaga ccggacatgg
      361 cgaccccagc cgcggctaaa accgctggtg gacggtccgc agagcgccgt cgtgacgggg
      421 ccggagggtg aggaaatctt ctgtgatgaa catggccgag tgcgggtgaa attcaactgg
      481 gaccgttata acccggcaga ccaggacagc tcgtgctgga tccgtgtggc acaggcgtgg
      541 gcaggcaccg gttttggcaa cctggcgata ccgcgtgtgg gtcaggaggt gat
//
LOCUS       DAZFKF010000373          582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFKF010000373 DAZFKF010000000
VERSION     DAZFKF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.379"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..376)
                     /locus_tag="ACHKA1_005618"
     CDS             complement(<1..376)
                     /locus_tag="ACHKA1_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001450959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed virulence protein BigA"
                     /protein_id="HFO5683104.1"
                     /translation="MQRKKLLSVCVAMALSSQTWAADTSIPDSTEKTRKSSKITCPAN
                     IHSLSKEQLENLSAECRENKDSAVLPWAAAGLAAIATGIAIYTLSDDDNHHHNNSPVP
                     DDGGDTPVPPDDGGDTPVPPDDG"
BASE COUNT          182 a          122 c          112 g          166 t
ORIGIN      
        1 cgccgtcatc tggcggaacc ggcgtatcac cgccgtcgtc tggcggaaca ggcgtatcac
       61 cgccatcatc cggaactggg gagttattat ggtggtgatt gtcatcgtca cttaaggtat
      121 atatagcaat tcccgtagct attgcagcaa gacctgcagc tgcccatggt aaaactgcac
      181 tatctttatt ttctctgcac tccgcggata aattttctag ctgctctttg ctcagggaat
      241 gaatatttgc cggacatgtt atcttactgg acttacgtgt tttttcggtt gagtcgggta
      301 ttgaagtatc cgcagcccat gtctggctac ttaacgccat ggcaacacaa acagacaata
      361 attttttcct ttgcatataa aattccttag atctagaccg tgcgtaacaa gccatgcaga
      421 atttgaagat aaatcgtagt aaatattcat agtgaagtaa gcaataaaga aatgcttata
      481 taattcacaa aatcaatatt aaaatcaggc aagtaaaagc atacaaaaaa aacagttgat
      541 ggaattatat tccacaaaaa actcacttca atctaaaaat ta
//
LOCUS       DAZFKF010000374          555 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFKF010000374 DAZFKF010000000
VERSION     DAZFKF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.380"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            44..>555
                     /locus_tag="ACHKA1_005619"
     CDS             44..>555
                     /locus_tag="ACHKA1_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5683105.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAITPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNAV"
BASE COUNT          166 a          103 c          133 g          153 t
ORIGIN      
        1 ttaaaggcat tatcatattt acgaagagat caggatgata acgatgaaaa aaagtgtatt
       61 gacggcgttt ataactgtgg tatgtgcaac gtccagtgtt atggcagctg atgataatgc
      121 tatcacggat ggttcagtaa catttaatgg taaagttatt gctccagctt gtaccctggt
      181 cgctgcgacg aaagattccg tggtgacttt gccagatgtt agtgccacga agttacaaac
      241 caatggtcag gtttctggcg tgcaaactga tgtgccaatt gaattaaaag attgtgatac
      301 taccgtaaca aaaaatgcaa cgttcacctt taatggcact gcggatacta ctcagattac
      361 agcgtttgct aaccaggcct catctgatgc tgctacaaac gtggccctgc aaatgtatat
      421 gaatgatggt acaacggcca tcacgccaga cacagaaacc gggaatattt tgttgcaaga
      481 tggagatcag acgttgactt ttaaagttga ttatatcgct acggggaaag cgacttctgg
      541 taatgtgaat gcggt
//
LOCUS       DAZFKF010000375          554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFKF010000375 DAZFKF010000000
VERSION     DAZFKF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.381"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>554
                     /locus_tag="ACHKA1_005620"
     CDS             <1..>554
                     /locus_tag="ACHKA1_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFO5683106.1"
                     /translation="LQYCIDSMNEHTGKVPLKAYYSSPEDIQKHIPFELEQQFDNLQK
                     NPPPGTCVVACDKFGEALSVFFHRMEKEKLTHMAAIVQSQTHAMAVRLRIKKTPAGET
                     EYVVSFYDPNATNTAVRYKANNCDSFGSLRSFMNIKQAQKKWVITEICSDCVGITPYL
                     PREQAHLLSGINNELQPPLSPSAL"
BASE COUNT          191 a          114 c           98 g          151 t
ORIGIN      
        1 ctacaatatt gtatagattc aatgaatgaa catacaggga aagttccatt aaaggcttac
       61 tattcatctc cggaagatat acaaaaacat attccattcg agcttgagca gcaatttgac
      121 aatctacaaa aaaatccacc acccggtaca tgcgttgttg cctgtgataa gtttggggag
      181 gcattatcag tcttttttca cagaatggaa aaagagaagt taacgcatat ggcggcaatc
      241 gttcaatctc aaacacacgc tatggccgtc cgcttgagaa tcaaaaaaac gcctgctggt
      301 gaaacagaat atgttgtatc cttttatgat cctaatgcaa ccaatactgc agtacgctat
      361 aaagcaaaca actgtgattc ttttgggtca ttgcgatcct ttatgaatat taaacaggca
      421 caaaaaaaat gggtaatcac agaaatttgc tccgactgtg taggaataac tccttatctc
      481 cctcgagaac aagcccattt attaagtggt ataaataatg agttgcaacc accattatca
      541 ccatcagcat taca
//
LOCUS       DAZFKF010000376          551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFKF010000376 DAZFKF010000000
VERSION     DAZFKF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.382"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>551
                     /locus_tag="ACHKA1_005621"
     CDS             <1..>551
                     /locus_tag="ACHKA1_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO5683107.1"
                     /translation="LGDTPLVDFTRDRLHRETLRRFGRYELTTAYTPVGQLQRQHLNS
                     LQYDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGN
                     RLPDPELHPDSTLSMWPDNRIACDAHYLYRYDRHGRLTEKTDLIPEGVIRTDDERTHR
                     YHYDSQHRLVHYTRTQYAEPLVE"
BASE COUNT          130 a          194 c          144 g           83 t
ORIGIN      
        1 ctcggcgaca caccgctggt ggatttcacc cgcgaccgcc tgcaccggga aacgctgcgc
       61 agattcggcc gttatgaact caccaccgct tatacccctg tcgggcagtt acagcgtcag
      121 cacctgaaca gcctgcagta tgaccgcgat tacacctgga acgacaacgg cgaactcatc
      181 cgcatcagca gcccgcgcca gacccggagt tacagctaca gcaccaccgg caggctgacc
      241 ggcgttcaca ccaccgcagc gaatctggat atccgcatcc cgtatgccac agacccggca
      301 ggtaaccgcc tgcccgaccc ggagctgcac ccggacagca ccctcagcat gtggccggat
      361 aaccgtatcg cctgtgacgc gcactatctt taccggtatg accgtcacgg cagactgaca
      421 gagaaaaccg acctcatccc ggaaggggtt atccgcacgg atgatgagcg cacccaccgg
      481 taccattacg acagtcagca ccggctggtg cactacacgc ggacacaata tgcagagccg
      541 ctggtcgaaa g
//
LOCUS       DAZFKF010000377          509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFKF010000377 DAZFKF010000000
VERSION     DAZFKF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.384"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>509
                     /locus_tag="ACHKA1_005622"
     CDS             <1..>509
                     /locus_tag="ACHKA1_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO5683108.1"
                     /translation="PIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCT
                     GVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKD
                     EGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGL
                     RFAIREGGRT"
BASE COUNT          125 a          125 c          141 g          118 t
ORIGIN      
        1 ccgatcgaag acgtattctc catctccggt cgtggtaccg ttgttaccgg tcgtgtagaa
       61 cgcggtatca tcaaagttgg tgaagaagtt gaaatcgttg gtatcaaaga gactcagaag
      121 tctacctgta ctggcgttga aatgttccgc aaactgctgg acgaaggccg tgctggtgag
      181 aacgtaggtg ttctgctgcg tggtatcaaa cgtgaagaaa tcgaacgtgg tcaggtactg
      241 gctaagccgg gcaccatcaa gccgcacacc aagttcgaat ctgaagtgta cattctgtcc
      301 aaagatgaag gcggccgtca tactccgttc ttcaaaggct accgtccgca gttctacttc
      361 cgtactactg acgtgactgg taccatcgaa ctgccggaag gcgtagagat ggtaatgccg
      421 ggcgacaaca tcaaaatggt tgttaccctg atccacccga tcgcgatgga cgacggtctg
      481 cgtttcgcaa tccgtgaagg cggccgtac
//
LOCUS       DAZFKF010000378          507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFKF010000378 DAZFKF010000000
VERSION     DAZFKF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.385"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..251
                     /locus_tag="ACHKA1_005623"
     CDS             <1..251
                     /locus_tag="ACHKA1_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683109.1"
                     /translation="WQKNGGGTVQMVTATASDWNQPIATATCPSGKKVTGGGGMCSFS
                     NAILKRSSPSGNSAWVAGCSQNTNQNQYYSVTTYALCQ"
BASE COUNT          128 a          112 c          129 g          138 t
ORIGIN      
        1 cgtggcagaa aaatggcggc ggtacagtac agatggttac tgcgacagca agtgactgga
       61 atcagccaat tgccaccgcg acttgccctt caggaaagaa agttacagga ggtggtggaa
      121 tgtgcagctt ttcaaatgcc attctcaagc gttcttcccc ctccggtaac agtgcatggg
      181 tagctggttg cagccaaaac accaaccaga atcagtatta ttcagtcact acttatgcat
      241 tgtgtcagtg agtattctgg tatgaattag cggaagcagt gaacagtgat gtagtagctg
      301 ccatataacg taccgttata cgcactccag ttcatctcgc cgtttgcatc tggattgtct
      361 gtaggccaga cacgcgtcat cgcggtttcc tttccatcta tgcgatatgt attaacgcaa
      421 agtttgaatt ttcctagagc cttcgttgtg gttgggtaca aggtgaagga tgttactgat
      481 gtccacggcg cagatgagcc tattgat
//
LOCUS       DAZFKF010000379          503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFKF010000379 DAZFKF010000000
VERSION     DAZFKF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.386"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            31..>503
                     /locus_tag="ACHKA1_005624"
     CDS             31..>503
                     /locus_tag="ACHKA1_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFO5683110.1"
                     /translation="MTIHYTATLNNKHLSLTALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRP"
BASE COUNT          137 a          149 c          118 g           99 t
ORIGIN      
        1 acagcatccc gccgacaagg aaaaagaaca atgaccatcc actacactgc aacactgaat
       61 aataaacacc tgtccctgac cgccctgtac cctgacggta atcgggaggt gattgccatc
      121 ctgccagtca cagaactggg cgcactgatt aaccgtaata acaggctgat gcacctgtcc
      181 ttcagttccc cttttcgcga aataaccgaa acggtgcttg ttgataccgt tgatgcagaa
      241 cgtcccggac atcacatcgg cagaatgctc tcagcgattg atgcctgcct ggatgccgaa
      301 tatgccggaa aaggaaaaca tccctcccgg aaaaatggct tctggcggac actgatgtgt
      361 ggctttatcg ccggaattgc tctgccggcg acagttgcag gttaccactg gatgaaggca
      421 gagccccctt ccacgctggc ttaccctaca tctcaggaat ggagagctga accagtaacc
      481 gacacagaga caccacgccc aca
//
LOCUS       DAZFKF010000380          502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFKF010000380 DAZFKF010000000
VERSION     DAZFKF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.387"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>502
                     /locus_tag="ACHKA1_005625"
     CDS             <1..>502
                     /locus_tag="ACHKA1_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683111.1"
                     /translation="ILDMLDNLTMRQQVVMEEFLRVFTSMYKASAMSEAIMSNVFEQL
                     VQYQRKETERATAASSVGVIIGSGVGAAGAAASVVANYRSHPEKMVTLTPDQEHTINR
                     TDDDVQRASKREVREQQASLKQIDEKIKNTTKDTSLNEEQKEEALKSYDKEKETTLEK
                     IRDTNE"
BASE COUNT          190 a           89 c          112 g          111 t
ORIGIN      
        1 atatccttga tatgctagat aatttgacca tgcgacaaca ggttgtaatg gaggaatttt
       61 taagggtatt cacatcaatg tataaagcct ctgctatgtc tgaggctatt atgagtaatg
      121 tttttgaaca acttgtccag tatcagagaa aagaaaccga acgcgccaca gcagcaagtt
      181 cggtaggtgt tattatcggg tcaggagttg gcgcagccgg ggccgcggca tctgtcgtag
      241 caaattacag aagccatccc gaaaagatgg taacccttac acctgaccaa gagcatacta
      301 tcaatagaac tgatgatgat gtacagcgcg cgtcaaaaag ggaagttcga gagcaacaag
      361 cttctctcaa acaaatagat gaaaaaataa aaaatacaac gaaagatacg agtcttaatg
      421 aagaacaaaa agaagaggcg ctgaaatctt atgataaaga aaaagaaact actctagaaa
      481 agattagaga tacaaacgag aa
//
LOCUS       DAZFKF010000381          474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFKF010000381 DAZFKF010000000
VERSION     DAZFKF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.388"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>474
                     /locus_tag="ACHKA1_005626"
     CDS             <1..>474
                     /locus_tag="ACHKA1_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4225 domain-containing protein"
                     /protein_id="HFO5683112.1"
                     /translation="ELKMESETARKEYQLFRQGNYDKYITTDIFEEHGLIKYVNLSFD
                     IVASVGQVVGGVGALKFGKVVHSNRIKGIGVTLVAHGANNFYESLSPLFFNEYDSGPI
                     RELYRVIAKKMGGDINSADYAYSIVDFSITAYGGYSGMKIVPKYNRLIRPSLGNR"
BASE COUNT          163 a           64 c          102 g          145 t
ORIGIN      
        1 atgaattaaa aatggagtca gaaacagcaa gaaaagaata tcaacttttc agacaaggaa
       61 attatgataa atatatcact actgatattt tcgaagaaca tggtttgata aaatatgtga
      121 acttaagttt cgatattgtt gcatctgtag gacaggtcgt aggcggggtt ggcgcgttga
      181 aatttggtaa ggtagtgcat tcaaaccgaa taaaaggtat aggcgtcaca ttagttgctc
      241 atggggcgaa taatttttat gaaagcttat cacctttatt ttttaacgag tatgattcag
      301 gtccaatacg tgaactctac agagtaatag caaaaaaaat gggaggtgat attaattctg
      361 ctgattatgc atacagcatc gtggactttt cgattactgc atatggtgga tattcaggca
      421 tgaaaatcgt acctaagtat aatagattga ttcgtccttc attaggaaat agac
//
LOCUS       DAZFKF010000382          472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFKF010000382 DAZFKF010000000
VERSION     DAZFKF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.389"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>472
                     /locus_tag="ACHKA1_005627"
     rRNA            <1..>472
                     /locus_tag="ACHKA1_005627"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          118 a          113 c          142 g           99 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg ga
//
LOCUS       DAZFKF010000383          470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFKF010000383 DAZFKF010000000
VERSION     DAZFKF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.390"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..273)
                     /locus_tag="ACHKA1_005628"
     CDS             complement(<1..273)
                     /locus_tag="ACHKA1_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO5683113.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADN"
BASE COUNT          153 a           95 c           77 g          145 t
ORIGIN      
        1 gttatctgcc atttcatcgc taaaaaaaga gaaatgacca ataatattac tttttttcct
       61 caactcttcc tgatggcgat atccagcctc tgaatctaaa cctggaactg catcaaaaac
      121 aaccagtcca gcttctgctg ctttattcgc gaatttgcca gcgctactaa ttactacatc
      181 atgtccaatt acacgaaagg cctgagccag agaaatcaat gggtataaca aaccgtacag
      241 cggtgggcca ataaaaagaa tacgttttcg cattaaaaaa gtccgtcttc tgcttacctg
      301 tttatttttc tatctctaag aaaacataat taatattatt tttatgtgag cttatgcaca
      361 aaaataacca agtatattat cttctggtag agatacagga tacagtttgt aggagctttc
      421 ttttttgaac aatgatttgc aggacagtta tgctaaacta gcggaatcag
//
LOCUS       DAZFKF010000384          464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFKF010000384 DAZFKF010000000
VERSION     DAZFKF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.391"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>464
                     /locus_tag="ACHKA1_005629"
     CDS             <1..>464
                     /locus_tag="ACHKA1_005629"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683114.1"
                     /translation="SIQYGYFESEKLPCRMSVIVGRNGCGKSTTLARLSRVAYSSTQD
                     RKKEQIAKIGEILPEGLGFPKIINLSYSAFDSFQIPGCTFKEKKQLRQDILDGKGRYI
                     YCGVRDVGAELDYVLANVDENNMDIEFITLDRQERTILKPLEVLSEEFYGVL"
BASE COUNT          161 a           59 c          107 g          137 t
ORIGIN      
        1 attcaataca atatggatat tttgaaagtg aaaaactacc atgtagaatg tccgtaattg
       61 taggccggaa cggttgtggg aagagtacaa cattggcaag actttctagg gttgcttatt
      121 cttcaactca ggacagaaaa aaagaacaga tagccaaaat tggtgaaatt cttccagagg
      181 gattaggttt tccgaaaata atcaaccttt catacagtgc ttttgatagt tttcaaatac
      241 ccgggtgtac ttttaaagaa aagaaacagc taaggcaaga catacttgat gggaaaggta
      301 ggtatatata ttgcggagtc agggatgttg gtgctgaatt ggattatgta ttggctaatg
      361 tagatgaaaa caatatggat atagagttta ttacattaga taggcaagag agaaccatat
      421 taaaacctct tgaagtactt agtgaagagt tttatggtgt cttg
//
LOCUS       DAZFKF010000385          464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFKF010000385 DAZFKF010000000
VERSION     DAZFKF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.392"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..90
                     /locus_tag="ACHKA1_005630"
     CDS             <1..90
                     /locus_tag="ACHKA1_005630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HFO5683115.1"
                     /translation="AARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            71..379
                     /locus_tag="ACHKA1_005631"
     CDS             71..379
                     /locus_tag="ACHKA1_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFO5683116.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            376..>464
                     /locus_tag="ACHKA1_005632"
     CDS             376..>464
                     /locus_tag="ACHKA1_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO5683117.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVL"
BASE COUNT          106 a          123 c          132 g          103 t
ORIGIN      
        1 gccgcacgtc ttgttgctgt gccggatgcc atccgttgtg tgaactgcca gaacatcatg
       61 gaggcccgtc atgtgggtca gcatcgttaa agattatgtt gtgccgatcc tttcggcgac
      121 ggcaaccgct ggcgggatat tcatggcgct gatgcgcaaa acgtttgtcc cccgcgaggc
      181 ctttgaaaaa ctctccgacc gcgttgagaa ggtggaaacc cgcctgtcgt cactgccgac
      241 agaagccgag gtcaaccgtc tgaatgtgga aatcgtgacc ctgcggggtg aactgaaaac
      301 cacgaacgcc acgctccgct ctgtctccta tcagaacgaa ctgctgctgg agcaggctgt
      361 aaggaaaaaa acgcaatgag tgatttcatt actgaagatc agcgtctggt cattctgcgg
      421 tcgctggcag attacaacgg tgaactgggt gaatccgtgc tcca
//
LOCUS       DAZFKF010000386          458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFKF010000386 DAZFKF010000000
VERSION     DAZFKF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.393"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>458
                     /locus_tag="ACHKA1_005633"
     CDS             <1..>458
                     /locus_tag="ACHKA1_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO5683118.1"
                     /translation="FGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAP
                     FVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQI
                     GTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLG"
BASE COUNT           90 a          135 c          128 g          105 t
ORIGIN      
        1 ttcggcgtga cctacaccgg taactatgag ttcccgcaac cgctgcacga tgcgctggat
       61 aaattccagg ccgacaccgg tatcgacatc gacatgcaca tcgacgccgc cagcggtggc
      121 ttcctggcac cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa
      181 tcgatcagtg cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc
      241 tggcgtgatg aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctcggtggt
      301 cagattggta ctttcgccat caacttctcc cgcccggcgg gtcaggtaat tgcacagtac
      361 tatgaattcc tgcgcctcgg tcgtgaaggc tataccaaag tacagaacgc ctcttaccag
      421 gttgccgctt atctggcgga tgaaatcgcc aaactggg
//
LOCUS       DAZFKF010000387          444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFKF010000387 DAZFKF010000000
VERSION     DAZFKF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.394"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..126
                     /locus_tag="ACHKA1_005634"
     CDS             <1..126
                     /locus_tag="ACHKA1_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFO5683119.1"
                     /translation="LYDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
     gene            complement(268..>444)
                     /locus_tag="ACHKA1_005635"
     CDS             complement(268..>444)
                     /locus_tag="ACHKA1_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO5683120.1"
                     /translation="EGTTVSHVQRLHHSLIAAGLESNLTVYTGGHDYAWWRGAIIDEL
                     ANYNCRKVSNNNSV"
BASE COUNT          152 a           83 c           84 g          125 t
ORIGIN      
        1 ctttatgatg attcactacg cttctgttca gatcaggtag ccgctgaaat ggctgaacaa
       61 cccagtcctg cagagattgc agaggttttg atgagaaaat taaaaaacaa cgggaaacaa
      121 ttgtagcgta ttgctgaaat tttcaacgag aaatgttatg agtaatataa tgtccttcat
      181 cacataaatg aaggacatca ctaaataaat gagatagtct cccataataa tattgcagaa
      241 agagttatcg gacgataaaa agatatttta cacagagtta ttatttgata ccttcctgca
      301 attataattt gctaattcat caataattgc tccgcgccac caagcataat cgtgaccacc
      361 ggtatagaca gtgaggttac tttccaaacc agctgcgatt aacgaatgat gcaatcgctg
      421 aacatgtgag acggttgtac cttc
//
LOCUS       DAZFKF010000388          434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFKF010000388 DAZFKF010000000
VERSION     DAZFKF010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.395"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            50..>434
                     /locus_tag="ACHKA1_005636"
     CDS             50..>434
                     /locus_tag="ACHKA1_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFO5683121.1"
                     /translation="MVDNVTVGGVCAQSPSFMPDLDGGKNKAQLIIDDIVAYLKNPSV
                     YSLEKEGPLNHFVNNCSEIEPWVLIGGGLIIIVSTSEQDPEGLIVTVGDIEGKCATQI
                     PVPPALTTSLKDIFIRPHTNAKYESF"
BASE COUNT          130 a           73 c           92 g          139 t
ORIGIN      
        1 tagtaacctt attattacta caactttcat atggatgagg ggaaagaaaa tggttgataa
       61 cgtaactgtt ggcggtgtat gtgcacaatc tccttctttc atgcctgatt tggatggagg
      121 aaaaaataaa gctcaattga tcattgatga tatagtggcg tatcttaaaa atccttcagt
      181 ttattcgctt gaaaaagaag gtccgttaaa tcattttgtt aataattgta gcgagattga
      241 accatgggtt ttaatcggtg gtggacttat tattattgtc tccacatctg agcaagaccc
      301 tgaaggtctg attgtaactg tgggggatat tgaaggaaag tgtgctacac agattcccgt
      361 accacctgcg cttacaacat ccctcaaaga tatttttatt cgccctcata cgaacgcaaa
      421 gtatgagagt ttta
//
LOCUS       DAZFKF010000389          431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFKF010000389 DAZFKF010000000
VERSION     DAZFKF010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.396"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT          103 a           98 c           94 g          136 t
ORIGIN      
        1 aaatgccagc gctggtattg tggaacccgg ccctgccctt ggtgctggcg atattcccgt
       61 tcatcttcct gctgatacct tccgttcagc agcgggttgt tatcccttcc tgactgattc
      121 gtcattccat tcttattgat tagaactggc attacaccgg tgccggcgtg ctttcctgcg
      181 tgtctgcgcc ggttcagtaa attagaaaag aaacatttgt cagcgccagt gatataaggc
      241 gtaacggcta acaatcttgg cgttaataca ttccgagcaa ataaccgaag cgttactttt
      301 agtgacgggg ttatccaata cttttctagt cagtgtttag tatttatgca agcgatataa
      361 gtagtcaccg cttagactat tgacattcat gatgcctgtt aattcaaatt tcaggtatga
      421 acagtcattt a
//
LOCUS       DAZFKF010000390          431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFKF010000390 DAZFKF010000000
VERSION     DAZFKF010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.397"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>431
                     /locus_tag="ACHKA1_005637"
     CDS             <1..>431
                     /locus_tag="ACHKA1_005637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO5683122.1"
                     /translation="TSGVLSFAEQTLPPGERERVMESFERVLMPGLEKNQYSILWVEH
                     QDKGRLELNFVIPNMELASGKRLQPYYDRADRPRINAWQTLVNHHYGLHDPNAPENRR
                     TLVTPNNLPKAKQEAAEAITRGLEALYHAGALKTRQDVTEAL"
BASE COUNT          108 a          112 c          136 g           75 t
ORIGIN      
        1 acctcggggg ttctgtcttt tgcagagcag accctgccac cgggagagcg tgaaagggtg
       61 atggagagct ttgagcgggt actgatgccc ggtcttgaaa aaaatcagta cagcatcctg
      121 tgggtggagc accaggacaa ggggcgtctt gagctgaatt ttgtcatccc gaatatggag
      181 ctggcgagcg gaaaacggct ccagccctat tacgaccggg cggaccgtcc gcgaatcaat
      241 gcctggcaga cactggtgaa tcaccattac gggctgcacg acccgaacgc accggagaac
      301 aggcggacac ttgtcacccc gaataatctg ccgaaagcga aacaggaggc cgcagaagcg
      361 attacgaggg gactggaggc tctttaccat gccggagcgc taaaaacgcg tcaggatgtc
      421 actgaggcgc t
//
LOCUS       DAZFKF010000391          429 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFKF010000391 DAZFKF010000000
VERSION     DAZFKF010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.398"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            213..>429
                     /locus_tag="ACHKA1_005638"
     CDS             213..>429
                     /locus_tag="ACHKA1_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFO5683123.1"
                     /translation="MAYHSPDITLHYPYLRRMSWAQITAAAEDAEIHHDYARALILWQ
                     HAYHAATLTLNKNLAVAKIDFCIKRIRM"
BASE COUNT          108 a          119 c          104 g           98 t
ORIGIN      
        1 ttttctggct ctcatccccc tttaatgtct cctccggcga tgcccggcaa cccacactgg
       61 ccgttcacag tcaggagttc cgtactgaaa aagcctgtcg ggatgccttc accgcgctga
      121 aaaaagaaaa cgtgggagaa ttcgctctcc ggggcgtctg cgtccccggc ggagaataaa
      181 ccggagatga tgattagcca caggagtatt caatggcata ccattccccg gatatcacac
      241 tgcattatcc gtatctgcgc cggatgtcct gggcgcagat aacggcagca gctgaagatg
      301 cggaaataca tcatgattat gcccgggcat taatattgtg gcagcatgca tatcatgctg
      361 ccacgctgac cctcaacaaa aacctggcag ttgctaaaat cgacttctgt attaaacgta
      421 tccggatgc
//
LOCUS       DAZFKF010000392          420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFKF010000392 DAZFKF010000000
VERSION     DAZFKF010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.denovo.399"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>420)
                     /locus_tag="ACHKA1_005639"
     CDS             complement(<1..>420)
                     /locus_tag="ACHKA1_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5683124.1"
                     /translation="YNNGYPTTYGNIIHLKGMTAVGEGELLIGWSGTSGAHAPAFIRS
                     RRDTTDANWSPWAQLYTSAHPPAEFYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPK
                     LAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIK"
BASE COUNT           99 a          113 c          110 g           98 t
ORIGIN      
        1 atttaatgcc gtcctgttcc tgagacaata cggcccgacc actggcaggt ttgcccttaa
       61 tcgtccagcc acgcatatca gggatcacgc ctgacggata agcggctgca agtttcgggt
      121 atgcagattt gtcaaaagtc tgcccctgca tcagggcata accagacgga acggtatctg
      181 atggccacgg gattggtgca ccgactggat aaaactctgc aggaggatga gccgaggtgt
      241 aaagctgcgc ccacggcgac cagtttgcgt cggtcgtatc ccgtcgtgaa cgaataaatg
      301 ccggagcatg agcaccgctt gtaccactcc agccgatgag taactcacct tcgccaacgg
      361 ctgtcatccc tttcaggtga atgatatttc catacgttgt tggatatccg ttgttataca
//
LOCUS       DAZFKF010000393        15367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.guided.110, whole genome shotgun sequence.
ACCESSION   DAZFKF010000393 DAZFKF010000000
VERSION     DAZFKF010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.guided.110"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(110..1822)
                     /locus_tag="ACHKA1_005640"
                     /pseudo
     CDS             complement(110..1822)
                     /locus_tag="ACHKA1_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            1794..2579
                     /gene="lafU"
                     /locus_tag="ACHKA1_005641"
     CDS             1794..2579
                     /gene="lafU"
                     /locus_tag="ACHKA1_005641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO5683125.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            2650..3705
                     /gene="dinB"
                     /locus_tag="ACHKA1_005642"
     CDS             2650..3705
                     /gene="dinB"
                     /locus_tag="ACHKA1_005642"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO5683126.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            3757..4050
                     /gene="yafN"
                     /locus_tag="ACHKA1_005643"
     CDS             3757..4050
                     /gene="yafN"
                     /locus_tag="ACHKA1_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFO5683127.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            4053..4451
                     /gene="yafO"
                     /locus_tag="ACHKA1_005644"
     CDS             4053..4451
                     /gene="yafO"
                     /locus_tag="ACHKA1_005644"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFO5683128.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            4461..4913
                     /locus_tag="ACHKA1_005645"
     CDS             4461..4913
                     /locus_tag="ACHKA1_005645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5683129.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
     gene            5104..5312
                     /locus_tag="ACHKA1_005646"
                     /pseudo
     CDS             5104..5312
                     /locus_tag="ACHKA1_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            5332..5640
                     /locus_tag="ACHKA1_005647"
                     /pseudo
     CDS             5332..5640
                     /locus_tag="ACHKA1_005647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
     gene            complement(5697..7154)
                     /gene="pepD"
                     /locus_tag="ACHKA1_005648"
     CDS             complement(5697..7154)
                     /gene="pepD"
                     /locus_tag="ACHKA1_005648"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO5683130.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREEVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            7415..7873
                     /gene="gpt"
                     /locus_tag="ACHKA1_005649"
     CDS             7415..7873
                     /gene="gpt"
                     /locus_tag="ACHKA1_005649"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO5683131.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            7965..9209
                     /gene="frsA"
                     /locus_tag="ACHKA1_005650"
     CDS             7965..9209
                     /gene="frsA"
                     /locus_tag="ACHKA1_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO5683132.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            9267..9668
                     /gene="crl"
                     /locus_tag="ACHKA1_005651"
     CDS             9267..9668
                     /gene="crl"
                     /locus_tag="ACHKA1_005651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO5683133.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(9707..10762)
                     /gene="phoE"
                     /locus_tag="ACHKA1_005652"
     CDS             complement(9707..10762)
                     /gene="phoE"
                     /locus_tag="ACHKA1_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO5683134.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            11050..12153
                     /gene="proB"
                     /locus_tag="ACHKA1_005653"
     CDS             11050..12153
                     /gene="proB"
                     /locus_tag="ACHKA1_005653"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO5683135.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            12165..13418
                     /gene="proA"
                     /locus_tag="ACHKA1_005654"
     CDS             12165..13418
                     /gene="proA"
                     /locus_tag="ACHKA1_005654"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO5683136.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLDKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCSLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRV"
     gene            13533..13608
                     /locus_tag="ACHKA1_005655"
     tRNA            13533..13608
                     /locus_tag="ACHKA1_005655"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13566..13568,aa:Thr,seq:cgt)
     gene            complement(13735..14142)
                     /locus_tag="ACHKA1_005656"
     CDS             complement(13735..14142)
                     /locus_tag="ACHKA1_005656"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5683137.1"
                     /translation="MVVASPSYLAANSTPVHPRELVNHKCIGWRQSPDVAPYRWPFEE
                     NRRAFDLAIEPQITTNDLRLMLRLALAGGGITIATQETFRPYIESGRLVSLLDDFLPQ
                     FPGFYLYFPQRRNIAPKLRALIDHVKEWRQQLA"
     gene            14148..14414
                     /locus_tag="ACHKA1_005657"
                     /pseudo
     CDS             14148..14414
                     /locus_tag="ACHKA1_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            14580..14858
                     /locus_tag="ACHKA1_005658"
     CDS             14580..14858
                     /locus_tag="ACHKA1_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683138.1"
                     /translation="MPMYIKLILSIVRRISVNTICSLIVVVTLSLLSFSSVAKTITAV
                     GSTIDSTEKEISLQAEKQGKSYKILGAFFKNRVYMIAKLTPVSKNDAS"
     gene            complement(15262..15324)
                     /locus_tag="ACHKA1_005659"
     CDS             complement(15262..15324)
                     /locus_tag="ACHKA1_005659"
                     /inference="COORDINATES: protein motif:HMM:NF041474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5683139.1"
                     /translation="MNIVVFVATVEYAYLSLHFY"
BASE COUNT         3834 a         3803 c         3930 g         3800 t
ORIGIN      
        1 cctgatgcga cgctggcgcg tcttatcatg cctacaaacc tgtgccggat cggtaggccg
       61 gataaggcgt tcacgccgca tccggcggtt gtgcaccgat gccggatacc tacatcaacg
      121 cccccataat cttcaactcc agctcatccg gcacttcgtt atacgacagc acatgcagcc
      181 ccggcgcaaa caatctcgca tagcgcgcca gcaaaggacg caactgcggc ggcaccagca
      241 gcaccgggtc tttccccgcc gctttcatct gctcttttac ctgcggcatc gtactctgga
      301 actggttgag catattcggg tcaaccggca cgctgtcgag catcactttg ccgccctgtt
      361 gcgcctgatt caccacattg gtgagcagat tttccagctc attattcagc gtatacaccg
      421 tcagctcctg cttgcgaacg aacggatggg taatgctgcg ccgcagcgcc aggcgtacat
      481 cggccgccag cagaatatga tctttagtca ccgcgctact ggcgaccagc acggtggcga
      541 tggtgacgat atcgcgcagg gaaacgcctt cggtcagtag tgtgcggtac actttcagca
      601 actggctgta attcagcgcc gcgcttaagt cttctgccag gcgcggtgcc atcgacgaca
      661 aacggttatg caactgtgtg atgtcgtcat agttaaacaa atcaggaata tagctgcgta
      721 caatcttatt cacatgggtg gcaatcacgc tggcgctgtc gatcacctga taccccatat
      781 tcagcgcctt cgctttctgc gcaggctgga tccaggtcac cggcatcccg tacgccgggt
      841 cgttccccag cacgccgtca atctcgccgt aggtttcgct ggagggcagg gccatcagtt
      901 tatccgccgg aatatccgct tcatcagcct taatgccgtt gatgaaaata gcgtactggc
      961 tgggcttaag gcggaagttt tcccgaatgc ggatctccgg cagcagcacg ccgttgccgt
     1021 cggatatcac ctgtcgcacg ccgcgaatac gctgggtgag cgggttgccc tgggctttgt
     1081 ccaccagcgc caccagcttg taaccgaggc ttaaactgat aggctcgatc agcggtatcg
     1141 tttcccaact gacctgttgc tcgctggttt cggtgatggt gcgggtcagc gtttcgagac
     1201 ttttctcctc cgcctccgct gcctgcgggc gtttgctcat ccgccagccg gtaaagccaa
     1261 gcagggcgct gaacagcaaa aacggcagat gcggcattcc cggcaccacc gccagcacaa
     1321 acataatccc ggtggcggtg tagagcaccg acgggcttgc cagcagctgg tggcgcacgt
     1381 catgggcgat atcgccgttg tcgctgacgc gggtgacgat aatcgccgcc gcggtggaga
     1441 gcagcaggga agggatctgc gccaccagcc cgtcgccgat ggtcatcagc acatattgct
     1501 ggaaggcagc atcggcgctc aggttgtatt tgaagatccc gatacagaca ccgccgatca
     1561 ggttgatcgc cagaatcatc atcccggcga tggcgtcccc gcgcacaaac tttgatgccc
     1621 cgtccatcgc gccgtagaaa tcggcctcgc tggcgacatc tttacgccgg gtttgcgcct
     1681 gcgcctggtt gatcaacccg gcgttaaggt cggcgtcaat cgccatctgt ttgccgggca
     1741 tcgcgtccag ggtaaagcgg gcagaaacct cggaaatacg ctcggcccct ttggtgacga
     1801 cgataaagtt gattgtcaac agcgtcagta aatccgagcg tgaaagcatt atcgccgcgc
     1861 tgcacgggca gtcgattttt aacggtggtg ggctgtcgcc gttaaataaa atcagcccgt
     1921 ctcatccgcc aaaacctgcc accgtggcgg tgccggaaga gacggagaaa aaagcgcgtg
     1981 atgtgaatga gaaaacggcg ctgctgaaga agaaatccgc caccgagctt ggcgagttgg
     2041 caaccagtat caacaccatt gcccgtgatg cgcatatgga agcgaatctg gagatggaga
     2101 ttgtcccgca gggattacgc gtgctgatta aagacgacca gaatcgcaat atgttcgaac
     2161 gcggcagcgc gcagattatg ccgttcttta aaactttgct ggtggagctc gcgccagtgt
     2221 tcgactcgct cgataacaaa attattatta ccgggcatac cgatgcgatg gcctacaaaa
     2281 acaatatcta caacaactgg aacctttcgg gcgatcgcgc gctttcagct cgtcgggtgc
     2341 tggaagaggc cggaatgccg gaagataaag tgatgcaggt aagcgcaatg gcggaccaga
     2401 tgctgctgga tgccaaaaat ccgcaaagcg cgggcaatcg gcgtattgag attatggtgc
     2461 tgaccaaaag tgcgtccgat acgctgtatc aatactttgg tcagcatggg gataaagtgg
     2521 tgcagccgct ggtgcaaaag ctggataagc agcaggtgct ttcgcagcga acgcgttaaa
     2581 tgctgaatct ttacgcattt ctcaaaccct gaaatcactg tatactttac cagtgttgag
     2641 aggtgagtaa tgcgtaaaat cattcatgtg gatatggact gctttttcgc cgcagtggag
     2701 atgcgcgaca atcccgccct gcgcgatatc cctattgcta ttggcggcag ccgcgaacgt
     2761 cggggggtga tcagcaccgc caattatccc gcgcgtaaat ttggcgtacg cagcgctatg
     2821 ccgacaggga tggcgctcaa attatgccca catctcacct tgcttccggg gcgctttgac
     2881 gcctacaaag aagcctcaaa tcatatccgt gaaatcttct cgcgctacac ctctcgcatt
     2941 gagccgttgt cactggatga ggcttatctc gatgtcaccg acagcgtcca ttgccacggt
     3001 tctgcgaccc tcattgccca ggaaatccgc cagacgattt tcaacgagct gcaactgacg
     3061 gcgtctgcgg gcgttgcgcc ggtaaagttt ctcgccaaaa tcgcctccga catgaataaa
     3121 cccaacggcc agtttgtgat tactccggca gaagttccgg catttctgca aaccttaccg
     3181 ctggcaaaaa tccccggcgt cggcaaagtc tcggcggcaa aactggaagc gatggggctg
     3241 cggacctgcg gtgatgtgca aaagtgtgat ctggtgatgc tgcttaaacg ctttggcaaa
     3301 tttggccgca ttttgtggga gcgtagtcag gggattgacg aacgcgatgt taacagcgaa
     3361 cggttgcgaa aatccgtcgg cgtggaacgc acgatggcgg aagatattca tcactggtct
     3421 gaatgtgaag cgattatcga gcggctgtat ccggaacttg aacgccgtct ggcaaaggta
     3481 aaacctgatt tactgattgc tcgccagggg gtgaaattaa agttcgacga ttttcagcaa
     3541 accacccagg agcacgtctg gccgcggctg aataaagctg atctaatcgc caccgcgcgt
     3601 aaaacctggg atgaacgccg cggtgggcgc ggtgtgcgac tggtggggct gcatgtgacg
     3661 ttgcttgacc ctcaaatgga aagacaactg gtgctgggat tatgatgtat actattatgt
     3721 ctattctggt gtgcattatt atgaggggat cactgtatgc atcgaattct cgctgaaaaa
     3781 tcggtcaata tcactgagtt acgtaaaaac ccagctaaat actttattga tcaaccggtt
     3841 gcggttcttt ctaataatcg ccccgcagga tatctcttaa gtgccagcgc attcgaagcg
     3901 ttaatggaca tgcttgctga acaagaggag aaaaagccca taaaggcgcg cttccgtcca
     3961 agtgctgcaa gattagagga aattacacgc cgcgctgaac aatatcttaa tgatatgacg
     4021 gatgatgatt tcaatgactt taaggaataa ggatgcgggt attcaaaaca aaacttattc
     4081 gcctgcaact tacagcagag gaacttgatg cgttaacggc ggattttatt tcctataagc
     4141 gtgacggtgt tttgccagat atatttggtc gcgatgcact ctacgacgac tcgtttacct
     4201 ggccattaat caaatttgag cgagttgctc atattcatct ggcaaatgtg aataatccat
     4261 ttccgccaca gttgcgccaa ttcagcagaa cgaatgacga agcgcatttg gtatattgtc
     4321 agggtgcgtt tgatgagcaa gcatggttgc tcattgccat tctgaaacct gaacctcata
     4381 aactggctcg agataacaac caaatgcata aaattgggaa aatggcagaa gcgtttcgca
     4441 tgcgtttttg aatttatatt atgaataaca tacaaataag aaactatcag cctggcgatt
     4501 ttcagcaact atgcgctatt ttcattagag cggttacgat gacagccagt cagcattatt
     4561 caccacaaca aattgctgcc tgggcgcaga ttgacgaatc tcgctggaag gagaaactcg
     4621 cgaaatcaca agtgcgggtt gcagtcatta atgcacaacc ggttggtttt atttcccgca
     4681 ttgaacatta tatcgatatg ttatttgttg accctgaata cacccgccgt ggggttgcca
     4741 gtgctttgtt aaaaccttgg attaagtctg aatccgaact tacggtggac gccagcataa
     4801 ccgcaaaacc cttttttgaa cgttatggtt ttcagacagt taagcagcag cgcgttgaat
     4861 gccggggagc gtggtttact aatttttata tgcgatataa accgcaacat taaatccagc
     4921 ttgcaatgaa aataacgccc gcctggtatg tgcgcgtttt ctgattccac aaactgcaag
     4981 gaggtaaatc atgacaaatc ctttatcaat gactctttgc agacctttcc aggattaatt
     5041 ctttttttct tgccctggat tcgtctgcca tttcctgatt tttattcata aatggaatgg
     5101 tacatgggca aatatattcg tcccttatcc gatgcggtat ttaccatcgc atctgatgac
     5161 ctgtggatcg agagtttagc gatccaacaa ttacacacca cggcaaattt acccaacatg
     5221 cagcgcgtag tggggatgcc agatttacac accccggacg cggctacccg attggcgcag
     5281 cgttcttctc tgttggtcgt ttttacccgg caagacgtcg cggtaacggt gctggaaacc
     5341 gaagccgggc gttatcctga cacgctgcgt tcgtcggggc cgggcggtca aaatgtcaat
     5401 aaaaccgact tggcggtacg cgccacgcat ctggcatccg gtattagcgt gaaggttcag
     5461 tcagagcgca gccagcatgc taacaaacgg ctggcgcgat tgctgattgc ctggaggctg
     5521 gagcagcagc aacaggaaaa tagcgcggcg ctgaaatcac agcggcgaat gttccatcac
     5581 cagattgaac gtggcaaccc gcgacgaacg tttacaggga tggcttttat cgaaggataa
     5641 tgaaggatga aactgccgga aggcgattaa acgccatccg gcagcaaatc aaataattac
     5701 ttcgccggaa tttctttcag cagttcagtc agcagtgtcc agtaatgacc tacgctttcg
     5761 atgtgaactt gctcatccgg agagtgtgga ccggtgattg ttggcccgat agaaaccatg
     5821 tccatttccg gatacggttt tttgaacaga ccacattcca ggcccgcgtg gataatctgg
     5881 atgttcggcg tcttgttgaa cagacgctga taggtttcac gtaccagatg catcaccgga
     5941 gagtttgcgt ccggctgcca gccaggatat gcacctttcg cttcggtttt cgcgccagcc
     6001 agtttaccca gcgaatccag catgctcacc acgtagtctt taccgctgtc gatcagagaa
     6061 cggatcagac agtgaatttc tacattattg tcagtcatgg tcaccacacc gacgttcagg
     6121 gaggtttcga ccacaccttt tgccacgtcg gaattgcgga tcacaccgtt cggggtggcg
     6181 ttcagcagac gaataaaggt atcgcgagat ttcgcaatca gggcagcttt atcgttcgca
     6241 acagagtcca gcaacaaggc cagatttttc tctttctctg ccagctcgtt tttcaggatc
     6301 tcctgatagg tattcaccag agatttcagg gcgtcgactt tatcagccgc gacagcaatg
     6361 gtcgcaaagg cttcacgcgg gatggcgtta cgcagtgtgc cgccgttgaa atcaataaga
     6421 cgcagatcca gttcttctgc atgacccgcc aggaagcgca ccagcagttt gttggcatta
     6481 ccgaggccaa cgtggatttc accgccggag tgaccgcctt tcagaccttt taaggttaac
     6541 ttgaaggttt caaaaccagc tggaacctct tcacgatcta aatgcaggtt ggaggtgaag
     6601 tcgatacccc ccgcgcaacc catgtagatt tcaccttctt cttcggagtc ggtgttaatc
     6661 agaatatcag cctgcaacca gttgctctgt aagccgaacg caccgtccat accggcttct
     6721 tcggtcatgg tcagcagcac ttccagcggg ccgtgaacca cgttttcgtc agccagaacc
     6781 gccagcgcag aggccatacc aatgccgtta tccgcaccca gcgtggtgcc gcgcgcttta
     6841 acccattcgc catcaatata aggctggata ggatctttcg tgaagtcatg cacggtgtcg
     6901 ttatttttct gcggcaccat atcgaggtgg gcctgcaaga cgaccggttt acgattttcc
     6961 atacctgctg tagcaggttt acgaatcaag atattaccta cctgatcgcg ttcgacatgg
     7021 aaacctttct cttttgccca accaacaatg tattcagcga gttgctcttc atgataggac
     7081 gggtgaggaa tagaacagat tttggcaaaa atatcccaca gcggctgtgg agataattga
     7141 gacagttcag acacgttaag tctccttgtc gatcacccgc aaaacagtat tgcaggtcac
     7201 agggttagca gaaaatgttg tcaacacaag acaggcttgc gagatatgtt tgagaatacc
     7261 actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact
     7321 ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt
     7381 ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg
     7441 ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg
     7501 gaaaggcatt attgccgtaa gccgtggcgg tctggtaccg ggtgcgttac tggcgcgtga
     7561 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
     7621 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
     7681 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
     7741 tgtcaccatc tttgcaaaac cggctggtcg tccgctggtt gatgattatg ttgttgatat
     7801 cccgcaagat acctggatcg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
     7861 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
     7921 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
     7981 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
     8041 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
     8101 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
     8161 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
     8221 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc acccaggcga
     8281 tggtgtggca acaaaaagcc tgtgcggaag aagatccgca actcagtggt cgtcactggc
     8341 tgcatgcggc tacgttgtac aacattgccg cctatcctca tctgaaagga gatgacctgg
     8401 ccgagcaagc gcaggctttg tcaaaccgcg cctatgaaga ggccgctcag cgtctgccgg
     8461 gcacgatgcg gcagatggag tttaccgtac ccggcggtgc gcccatcacc ggctttttgc
     8521 atatgccgaa aggcgatggc ccgttcccga cagtattaat gtgtggtggt ctggatgcga
     8581 tgcagacgga ctattacagc ctgtatgaac gttattttgc gccgcgcggc attgcgatgc
     8641 tgactattga tatgccgtcg gtgggctttt catcaaaatg gaagctcacc caggactcca
     8701 gcctgttgca tcagcacgtc ttaaaggcgc tgcctaacgt accgtgggtg gatcacactc
     8761 gcgtcgcggc ctttggtttc cgtttcggcg ctaacgttgc cgtgcgtctg gcatatcttg
     8821 aatcgccgcg tctgaaagcg gttgcctgtc ttggtccggt agttcatacc ctgttgagtg
     8881 attttaagtg ccagcaacag gtgccggaaa tgtatcttga cgttctggcg agtcgtttgg
     8941 ggatgcatga tgcttccgat gaagcgttgc gcgtggagct gaatcgctat tcattaaaag
     9001 tgcaaggatt gctgggacgt cgctgcccaa cgccaatgtt atcaggctac tggaagaacg
     9061 atccgttcag cccggaagag gactcacgct taatcacctc atcatctgct gacggtaaat
     9121 tattagagat cccatttaac ccggtgtatc ggaattttga caaaggtctt caggaaatca
     9181 ccgactggat cgaaaaacgc ttgtgttaaa aatttgctaa attttgccaa tttggtaaaa
     9241 cagttgcatc acaacaggag atagcaatga cgttaccgag tggacacccg aagagcagat
     9301 tgatcaaaaa atttaccgca ctaggcccgt atattcgtga aggtaagtgc gaagataatc
     9361 gattcttttt cgattgtctg gctgtatgcg tcaacgtgaa accggcaccg gaagtgcgtg
     9421 agttctgggg ctggtggatg gagcttgaag cgcaggaatc ccgttttacc tacagttacc
     9481 agtttggtct gttcgataaa gcaggcgact ggaagagcgt tccggtaaaa gacactgaag
     9541 tggttgaacg actggagcac accctgcgtg agtttcacga gaaactgcgt gaactgctga
     9601 cgacgctgaa tctgaagctg gaaccggcgg atgattttcg tgacgagccg gtgaagttaa
     9661 cggcgtgagt gaaatgtgcc ggatgcatca catccggcaa taatcattaa aactgatagg
     9721 tcatgccaac cgcgacaata tcatcattat taatattcaa tttgttatcg ctatccagtt
     9781 ggttgatttt ataatcaaca aacgctgaca tatttttgtt gaaataatat gtagccccga
     9841 catcgatata attgaccaga tcttcatcac cgataccttc aatatctttc cctttcgata
     9901 agacataacc cagcgatgga cgcagaccaa aatcaaactg gtattgagcg accgcttcaa
     9961 agttctgtgt cttattggca aagccgccag ttattggcgt cattttgcgt gtttcagaat
    10021 aaaaagttgc cagataaata ttattggcat cgtatttcag acctgttgcc cacgcttctg
    10081 cacgcttgcc tgtgccacgg ctttgcaggt tctgctcgtt ggtgcgatct gagttggtat
    10141 aggccccact aatggcgaaa tcgctgccgc caaagtcata tgtcaatgac gtgccgaagc
    10201 catcgccgtt ttgcttttta acgtcgcggt tttcgttttt cccttgatat tgcagggtta
    10261 agttcaggcc atcgataacg ccgaagaagt cggtgttccg atacgtcgcc agaccgctgg
    10321 cgcgtttggt cataaagttg tcggtctgcg ccgaggagtc gccaccaaat tccgggaaca
    10381 tatcggtcca ggcttccacg tcatacaacg cgcccaggtt acgaccataa tcgaaagaac
    10441 ccaaatcttt atatttcaat ccggcaaaag cgagacgcgt tttttgctgt gcagtatcac
    10501 tctctgcttt attaccggca aactctgctt cccaacgacc ataaccagtc agttgatcgt
    10561 taatttgtgt ttcgccttta aaaccaaaac ggatataact ctggtcgcca tctttactgt
    10621 cgttatcact catataatgc atggctttaa ctttgccata gacatccagt ttattaccgt
    10681 ctttgttata tatttctgcg gcctgtacgg atgcagatgc cacaatgccc atcaccacta
    10741 atgccagagt gctctttttc attttcattc ctgattttaa ttaacgcgcg aatattctgc
    10801 gggagagccc cgttgaaaac aggaaagttt ttaacctgag attgttaaag atatattaca
    10861 gattaatgat attcttaaaa tgtggtaatt tagtaaatct gtaataaaag cgtaaacaac
    10921 tgccgctacg cttgctgatc ccgcgcaaca aaacaccatg ctttgctcgc agatggttgg
    10981 caaccgacga cagtcctgct aaaacgttcg tttgatatca tttttcctaa aattgaatgg
    11041 cagagaatca tgagtgacag ccagacgctg gtggtaaaac tcggcaccag tgtgctaaca
    11101 ggcggatcgc gccgtctgaa ccgtgcccat atcgttgaac ttgttcgcca gtgcgcgcag
    11161 ttacatgccg ccgggcatcg gattgttatt gtgacgtcgg gcgcgatcgc cgccggacgt
    11221 gagcacctgg gttacccgga actgccagcg actatcgcct cgaaacaact gctggcggcg
    11281 gtagggcaga gtcgactgat tcaactgtgg gaacagctgt tttcgattta tggcattcac
    11341 gtcgggcaaa tgctgctgac tcgtgctgat atggaagacc gtgaacgctt cctgaacgcc
    11401 cgcgacaccc tgcgtgcgtt gctcgataac aatatcgttc cggtaatcaa tgagaacgat
    11461 gctgtcgcta cggcagagat taaagtcggc gataacgaca acctttctgc actggcggcg
    11521 attctggcgg gtgccgataa actgttgtta ctgaccgatc aaaaaggttt gtataccgct
    11581 gacccgcgca gtaatccgca ggcagaattg ataaaagatg tttacggtat tgatgacgca
    11641 ctgcgcgcga ttgccggtga cagcgtttca ggcctcggaa ctggcggcat gagtaccaaa
    11701 ttgcaggccg cggacgtggc ttgccgtgcg ggtatcgaca ccattattgc cgcgggcagc
    11761 aagccgggcg tcattggtga tgtgatggaa ggcatttccg tcggtacgct gttccatgcc
    11821 caggcgactc cgcttgaaaa ccgtaaacgc tggattttcg gtgcgccgcc tgcgggtgaa
    11881 atcacggtag atgaaggggc aaccgccgcc attcttgaac gcggcagctc cctgttgccg
    11941 aaaggcatta aaagcgtgac tggcaacttc tcgcgtggtg aagtcatccg catttgtaac
    12001 ctcgaaggtc gcgatatcgc ccacggcgtc agtcgttaca acagcgatgc attacgccgt
    12061 attgccggac accactcgca agaaattgat gcaatactgg gatatgaata cggcccggtt
    12121 gccgttcacc gtgatgacat gattacccgt taaggagcag gctgatgctg gaacaaatgg
    12181 gcattgccgc gaagcaagcc tcgtataaat tagcgcaact ctccagccgc gaaaaaaatc
    12241 gcgtgctgga taaaatcgcc gatgaactgg aagcacaaag cgaaatcatc ctcaacgcta
    12301 acgcccagga tgttgctgac gcgcgtgcca atggcctcag cgaagcgatg cttgaccgtc
    12361 tggcactgac gcccgcacgg ctgaaaggca ttgccgatga tgtgcgccag gtgtgcaacc
    12421 tcgccgatcc ggtggggcag gtaatcgatg gcggcgtact ggacagcggc ctgcgtcttg
    12481 agcgtcgtcg cgtaccgctg ggggttattg gcgtgattta tgaagcgcgc ccgaacgtga
    12541 cggttgatgt cgcttcgctg tgcctgaaaa caggtaacgc ggtgatcctg cgcggtggca
    12601 aagaaacctg tcgcactaac gctgcaacgg tggcggtgat tcaggacgcc ctgaaatcct
    12661 gtagcttacc ggctggtgcc gtgcaggcga ttgataatcc tgaccgtgcg ctggtcagtg
    12721 aaatgctgcg tatggataaa tacatcgaca tgctgatccc gcgcggcggg gctggtttgc
    12781 ataaactgtg ccgcgagcag tcgacgatcc cggtgatcac aggtggtata ggcgtatgcc
    12841 atatttacgt tgatgaaagt gcagagatcg ctgaagcatt aaaagtgatc gtcaacgcga
    12901 aaactcagcg tccgagcaca tgtaatacgg tagaaacgtt gctggtgaat aaaaacatcg
    12961 ccgatagctt cctgcccgca ttaagcaaac aaatggcgga aagcggcgtg acattacacg
    13021 cagatgcagc tgcgctgaca cagttgcagg caggccccgc gaaggtggtg gcggttaaag
    13081 ccgaagagta tgacgatgag tttctgtcat tagatttgaa cgtcaaaatc gtcagcgatc
    13141 ttgacgatgc catcgcccat attcgtgaac acggcacaca acactccgat gcgatcctga
    13201 cccgcgatat gcgcaacgcc cagcgttttg ttaacgaagt ggattcgtcc gctgtttacg
    13261 ttaacgcctc tacgcgtttt accgacggcg gccagtttgg actgggtgcg gaagtggcgg
    13321 taagcacaca aaaactccac gcgcgtggcc caatggggct ggaagcactg accacttaca
    13381 agtggatcgg cattggtgat tacaccattc gtgtgtaaat aaaaccgggt gatgcaaaag
    13441 tagccatttg attcacaagg ccattgacgc atcgcccggt tagttttaac cttgtccacc
    13501 gtgattcacg ttcgtgaaca tgtcctttca gggccgatat agctcagttg gtagagcagc
    13561 gcattcgtaa tgcgaaggtc gtaggttcga ctcctattat cggcaccatt taaatcaagt
    13621 agttacccca tattcaaata caccacgttt cctcctgtgc cgtatttatg ccattgtaac
    13681 cctggcaatt aatcaaaata ctgttctgac atcaggcaat gcaggtgcag acatttaagc
    13741 caattgctgc cgccattcct tgacgtggtc aatcagggcg cggagctttg gtgcaatatt
    13801 gcgacgctgt gggaaataca gatagaagcc cggaaattgt ggaagaaagt catcaagcag
    13861 agatacaagc ctaccgcttt caatatatgg cctgaaagtt tcctgagtgg caattgttat
    13921 tcctccgccg gcaagagcca gcctcaacat cagacgcaga tcattagtcg taatctgcgg
    13981 ttcaatcgca aggtcgaaag ctctcctgtt ttcttcaaat ggccagcgat aaggcgcaac
    14041 atccggggat tgacgccagc cgatacactt atgattcaca agttcacgcg gatgaacagg
    14101 tgtgctgttg gctgcgagat aggagggaga ggcaacgacc atttcacgct gcctgatttg
    14161 cagtacgggc tatttctgat ggcttacggc aggaaagctc gaaaatccgt gtgacctgcg
    14221 taaatcctgg cgttgtcgaa agtgaactgg cttcaaccat tacccatgtg gaaaccatga
    14281 aggcaatgga cgcctaccgt tctgttgcac ttcaacctgc ggatattgcc cgtgcggtaa
    14341 ggcatattat tgaatctccc gaaagcgttg atacaaccga aattaccatc agacccactg
    14401 catctgcaaa ctgaagaata acttttacat tcatgtttta ttaaataggt cagaaggaag
    14461 atttatatat ccctgtgttt ttcatagcaa gatgcattat gtattgctca ccccgaagga
    14521 gatgaaggtt taattacaga aggagtattt tctcttgggg taaatatgac tcatgtttaa
    14581 tacctatgta tataaaactc atcctctcaa ttgtcaggag aattagcgtg aatactatct
    14641 gttctttaat tgttgttgtt acactgtctt tattatcatt cagtagcgtc gccaaaacga
    14701 ttactgccgt cggttcaacc attgacagca ctgaaaaaga aatttcttta caggcagaaa
    14761 aacaagggaa atcatataaa attctgggcg cgttttttaa gaacagagtt tatatgatag
    14821 caaaattaac accagtcagt aaaaatgatg cttcataagg ctcatggtat aatttttaag
    14881 acatgatcaa gaagcacgtc tattgactca aaattgtcat ttgtcaggca atggaatacc
    14941 tgacaaatga cgctatccca atctattgta ccagtcgttc tgctaattct accgatgctt
    15001 cggtctggag tggtagagta atattggaca cagttttatt ttctctcttt ttcgagggct
    15061 gtatatcaac aggaagaata catccctaag cggttaatgc tggcgtaatc aatagaccag
    15121 cattaatcac agatgattac caatcagaca gaaatggtta aatcgtcgaa ttcatattta
    15181 atacgttttc ttatgccgct gaacagcttg aaaaggatgc atattttaaa tatcagtgaa
    15241 atgaacagtt tattgccgtt attaatagaa atggagggat aaatacgcgt attctactgt
    15301 tgcaacaaaa acaacgatat tgatcggttt tcataatgaa gtaacctgac agatatgaaa
    15361 atgttga
//
LOCUS       DAZFKF010000394        15255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.guided.112, whole genome shotgun sequence.
ACCESSION   DAZFKF010000394 DAZFKF010000000
VERSION     DAZFKF010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.guided.112"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHKA1_005660"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHKA1_005660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO5683140.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="ACHKA1_005661"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="ACHKA1_005661"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO5683141.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="asd"
                     /locus_tag="ACHKA1_005662"
     CDS             2040..3008
                     /gene="asd"
                     /locus_tag="ACHKA1_005662"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO5683142.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="ACHKA1_005663"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="ACHKA1_005663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO5683143.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VIEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAGLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="ACHKA1_005664"
     CDS             complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="ACHKA1_005664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO5683144.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="ACHKA1_005665"
     CDS             complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="ACHKA1_005665"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO5683145.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9527..11335
                     /gene="frdA"
                     /locus_tag="ACHKA1_005666"
     CDS             9527..11335
                     /gene="frdA"
                     /locus_tag="ACHKA1_005666"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO5683146.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11328..12062
                     /gene="frdB"
                     /locus_tag="ACHKA1_005667"
     CDS             11328..12062
                     /gene="frdB"
                     /locus_tag="ACHKA1_005667"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO5683147.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12073..12468
                     /gene="frdC"
                     /locus_tag="ACHKA1_005668"
     CDS             12073..12468
                     /gene="frdC"
                     /locus_tag="ACHKA1_005668"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO5683148.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12479..12838
                     /gene="frdD"
                     /locus_tag="ACHKA1_005669"
     CDS             12479..12838
                     /gene="frdD"
                     /locus_tag="ACHKA1_005669"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO5683149.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12901..14034
                     /locus_tag="ACHKA1_005670"
     CDS             12901..14034
                     /locus_tag="ACHKA1_005670"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO5683150.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14123..14656
                     /gene="blc"
                     /locus_tag="ACHKA1_005671"
     CDS             14123..14656
                     /gene="blc"
                     /locus_tag="ACHKA1_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO5683151.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14653..14970)
                     /gene="sugE"
                     /locus_tag="ACHKA1_005672"
     CDS             complement(14653..14970)
                     /gene="sugE"
                     /locus_tag="ACHKA1_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO5683152.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15146..15253)
                     /locus_tag="ACHKA1_005673"
     CDS             complement(15146..15253)
                     /locus_tag="ACHKA1_005673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HFO5683153.1"
                     /translation="MLSTVLTACNTTRGVGEDISDGGNAISGAATKAQQ"
BASE COUNT         3755 a         4053 c         3975 g         3472 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc ctaaaaaatt ttttttgagt ttttatctca agtgctgaaa ttatcagcaa
      181 ggcgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccgacgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaagcgacg atcctggccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcacg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactgg tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg tgaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcggtgtt gacgcgtccg gatttctacg acggcgtgaa acctattgcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc actcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgaacagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctaaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggcaaa tccagcctgc
     1561 tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggcttggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt cctggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca ccgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca cggctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatctgc ctgttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggtgcgac gattgtaggc agcattgaga ctgtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggcat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcgc
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aacttggctc taccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaacca gccccacttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcacccagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cgcagccaga agtcatagag gcactccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaag caatatcaag
     3241 aagttattga taattatccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggga tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagtcagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaacga gatcgagcgc cgcctgggaa cgcttaccgg caatactccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactctg cacgtcttaa ggcgctcgtt gatgaactgg
     3601 agctggcgca gctgtctgcc aataaccgcc aggaattagc gcgcttgcgc tctgagctgg
     3661 ctgaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttgcgtaat caattgaaca
     3721 gccaacgcca gcttgaggca gagcgggcgc tggaaagtac cgaattacta gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcag
     3841 cgctgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa tctgctcggt gaagcgttgc gggcgcaggt ggcacggctg ccggaaatgc
     4021 cgaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaattca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacaactacg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacactac tgctggaact gaccaagctg aaagtctcta
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc gacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctattttacc cgttttctcg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttacgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggtgtaacgg ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccactga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgcga attttccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatacc gctgtatctc aacaaagagg gcagcggcga caatattacc aaccatatgt
     5041 tgtggaacat gatgattggc gcgccactgg ttgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc acaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcatgtt atccgccgct ggatgctgat ccagcgccgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca acgtgcacgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaaggag caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat tagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttcggt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcact tccacggtac agggcgtgga aagtctggag ccgattaccc
     5521 tcggtgcggg gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaatc
     5581 tgcccgcgct gctggaactg gcgattttgc agcacctgga tttaacgccg ggtaccggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 caatgattgg tattgagtgg tcgaaattgc agtggctggt cgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggcctgatc atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttactggt agcgtgacaa
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg taaagagata atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gattccggcc cctgccgatg ccaacagtga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcaccggaa gtcttcctgg
     6121 tggatctgca acaggggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagatccacc agctgattct ggctggcttc catgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctcaac ggtaaacaaa
     6301 cggggagaac gctgacgtct gcgggcaaag gtcgtcaggc gggaagtttg taatgcatga
     6361 agctgccaaa tgatgtcaac gtggctcatt tagcagctta acatcatctt cagttatcac
     6421 cggccctgga gagtatcatc catacgcatt acttcctgac aactcatcgc cggagagacg
     6481 atgagttgca ggatttcata tgatcagtcg agagctaact caggtttatt cgccatgcga
     6541 ctgcaatagt tctggtaaat cgccatcgct aacagcgaga agaagacagg gccgccaatc
     6601 atccacaatg tgctgtccca gtctccggct tcaaccacag gttgaatgat ggtgaagacg
     6661 ttcgcaaatg tcaccaccag tacgacaacc actgtcgcaa tcattgccga catacgcgtt
     6721 ttgaaaatca caaatggtct gtcgagatcc tgacgtgctt taaagaatgg gaaagccagc
     6781 gcgaggaaca ggtaaggaag cgtcatagac acgttcgcca tcagcgtcag cttgttaaag
     6841 aacgccgatg cggtaccgcc accaaacgaa accagcagga tgaagacagt aaccaacccg
     6901 cactgcatcc acatggcgat agacggcatc cccatcgcat tcaggcgcgt catcggttcc
     6961 ggccacaacg ctttcggcgt cccctggatg atggctttca gcggtgaata gcaaagcgta
     7021 aagaacgcac cggtataagc gaggaacatc gaaagtccgg taatacgcgc aaaccatacg
     7081 cccagcgaca atgacgcttc aggtgacaaa tgcagtgcgt tacccagcgt caccccgagg
     7141 ctcttcatca gcacataggt aatattgcca aggttaacgg aaccattact taatacctgc
     7201 tgccagtttg tgctgacgcc ccataaaaat attgccagcg aataaccgat tgaaataaca
     7261 atagcggcaa aaacaatacc tttggcaaag ttcttttctg gattttccgt tttatcgacc
     7321 agaccaccaa ccgcttcaat tccgccatag gcaaaaatag caaataccac aaacgatagc
     7381 attgccagac cggactgata acctgggttc ggtgatgcaa ggaaattaat atcctgcgcg
     7441 aaatgcccac catttaataa caaaatagta atgcttacta acagcaatac taaattcaga
     7501 cacattactg caataccccc caccgcagta atgcgggcaa ttttattaat cccctttgaa
     7561 gcaacgacgg tgaccagaat catccatgcc actgccagca gaccaaccac ctgcgtaggc
     7621 tccagtccgg caatacgcca gtgctgggtc atgtcgctac catagaggaa tgttgagaac
     7681 ggtacccaaa ctttcgcgga ggtgctcacc atccagatga tataagagga aaaccacata
     7741 aacgtaccaa tgaaggcaaa acgtggtccg acactattat tcatccagga atagataccg
     7801 ccttcttctt tgcgataagc agctcccatt tcagccatca ttaaggcgaa tggaataaag
     7861 aataacaatg cagaaaatat ataaaaggga atcgcactat aacccattaa gtaataagcc
     7921 gatgggctat tggcaaatcc aaatacggaa gtaaagatca tcaatatgag tcctatcaga
     7981 ctcatctttt ttatcgtgtg aggcatgaaa ccatccttca tttcagcgcg tgtataaaac
     8041 gcgcccactg aactgcactg cccaacattg agcgggcagc gtaaaaacca atacagaatt
     8101 gatacctgga tgatatcaaa tatccagtct taaattgtgg cctttagcgg aaaaatgaaa
     8161 acatatgcta cagaatggcg cttatcacgc cattcttcgc tgttaattca gtaatttttc
     8221 agaattatgc ccggtcaacg ctaaaggcga tgacttcagc cagtgtctcc gcacccagcg
     8281 ccaacatcac cagacgatca acgcctaatg ccacgccgga acagtcaggc ataccgactt
     8341 tcaaggcttc aatcagattc tggtcaatgg ggtgctgcgg caaaccgcgc gccgcgcgct
     8401 tacggttatc ttgttcaaag cgttgttgct gctcacgggc atccgtcaat tcatggaaac
     8461 cattcgccag ctcaatacct ttataataaa cctcaaagcg ttcagcgacc cgatgatctt
     8521 cggtactgat ttgcgccagt gatgcctggc tggctggaaa gtggtacaca aaggtcggtt
     8581 tttctttacc aatatttggt tctacgccaa aggtaaacag caattgtagc aacgtgtcgc
     8641 ggtcttcttc ggtatcagca acattgctca aatccagttt cgctgccact tcccgcagtt
     8701 gcgttttgtc ggcagagagc gggtcaattt ccagataacg caagaaagct tgttgataag
     8761 aaaggctttc tgctgccggg cagtccagca cctgttgtaa gagatcgtcc acctcgttca
     8821 tcaaccggta catatcatag tgcggtcgat accactcaag catagtgaac tcagggttgt
     8881 gataacgccc catctcttca ttacggaaac tgcggcacag ctggaatacc ggcccgcaac
     8941 cggcaaccag caggcgtttc atatggtatt ccgggctggt cattaaccag agattcatcc
     9001 cctgcgaatg cccggggcca acgaaacgtg tctcaaacgg gaccaaatga atatcggtta
     9061 ccgtcgcctg gctcatacaa ggcgtctcca cctccagcac tccacgatcg gcaaagaaac
     9121 gacggatctc cgccataatc gccgcgcgtt ttaataagtt aggaatggat gcgctcggct
     9181 gccaggatgc cgtttcgctc atagttaaat ctccagtttt tgacaagggc acgaagtcta
     9241 ctcgcaacgc gacggcgaga caaattttac gcaggaataa aacaacggtg ggcagtgact
     9301 aaaaaaagca cgatctgatg gtttagtaat taaattaatc atcttcagtg ataatttagc
     9361 cctcttgcgc actaaaaaaa tcgatctcgt caaatttcag acttatccat cagactatac
     9421 tgttgtacct ataaaggagc agtggaatag cgttcgcaga ccgtaacttt caggcactta
     9481 ccctgaagta cggggctgtg ggataaaaac aatctggagg aatgtcgtgc aaacctttca
     9541 agccgatctt gccattgtag gcgccggtgg cgcgggatta cgtgctgcaa ttgctgccgc
     9601 gcaggcaaat ccaaatgcaa aaatcgcact aatctcaaaa gtatacccga tgcgtagcca
     9661 taccgttgct gcagaagggg gctccgccgc tgtcgcgcag gatcatgaca gcttcgaata
     9721 tcactttcac gatacagtag cgggtggcga ctggttgtgt gagcaggatg tcgtggatta
     9781 tttcgtccac cactgcccaa ccgaaatgac ccaactggaa ctgtgggggt gcccatggag
     9841 ccgtcgcccg gatggtagcg tcaacgtacg tcgcttcggc ggcatgaaaa tcgagcgtac
     9901 ctggttcgcc gccgataaga ccggcttcca tatgctgcac acgctgttcc agacctctct
     9961 gcaattcccg cagatccagc gttttgacga acatttcgtg ctggatattc tggttgatga
    10021 tggtcatgtt cgcggcctgg tagcaatgaa catgatggaa ggcacgctgg tgcagatccg
    10081 tgctaacgcg gtcgttatgg ctactggcgg tgcgggtcgc gtttatcgtt acaacaccaa
    10141 cggcggcatc gttaccggtg acggtatggg tatggcgcta agccacggcg ttccgctgcg
    10201 tgacatggaa ttcgttcagt atcacccaac cggtctgcca ggttccggta tcctgatgac
    10261 cgaaggttgc cgtggcgaag gcggtattct ggtcaacaaa aatggctacc gttatctgca
    10321 agattacggc atgggcccgg aaactccgct gggcgagccg aaaaacaaat atatggaact
    10381 gggtccacgc gacaaagtct ctcaggcctt ctggcacgaa tggcgtaaag gcaacaccat
    10441 ctccacgccg cgtggcgatg tggtttatct cgacctgcgt cacctcggcg agaaaaaact
    10501 gcatgaacgt ctgccgttca tctgcgaact ggcgaaagcg tacgttggcg tcgatccggt
    10561 taaagaaccg attccggtac gtccgaccgc acactacacc atgggcggta tcgaaaccga
    10621 tcagaactgt gaaacccgca ttaaaggtct gttcgccgtg ggtgaatgtt cctctgttgg
    10681 gctgcacggt gcaaaccgtc tgggctccaa ctccctggcg gaactggtgg tcttcggccg
    10741 tctggccggt gaacaagcga cagagcgtgc agcaactgcc ggtaatggca acgaagcggc
    10801 aattgaagcg caggcagctg gcgttgaaca acgtctgaaa gatctggtta accaggatgg
    10861 cggcgaaaac tgggcgaaga tccgcgacga aatgggcctg gctatggaag aaggttgcgg
    10921 tatctaccgt acgccggaac tgatgcagaa aaccatcgac aagctggctg agctgcagga
    10981 acgcttcaag cgcgtgcgca tcaccgacac ctccagcgtg ttcaacaccg acctgctcta
    11041 caccattgaa ctgggccacg gtctgaacgt tgctgaatgt atggcgcact ccgcaatggc
    11101 acgtaaagag tcccgcggcg cacaccagcg tctggacgaa ggttgcaccg agcgtgacga
    11161 cgtcaacttc ctcaaacaca ccctcgcctt ccgcgatgct gatggcacga ctcgcctgga
    11221 gtacagcgac gtgaagatta ctacgctacc gccagctaaa cgcgtttacg gtggcgaagc
    11281 ggatgcagcc gataaggcgg aagcagccaa taagaaggag aaggcgaatg gctgagatga
    11341 aaaacctgaa aattgaggtg gtgcgctata acccggaagt cgataccgca ccgcatagcg
    11401 cattctatga agtgccttat gacgcaacta cctcattact ggatgcgctg ggctacatca
    11461 aagacaacct ggcaccggac ctgagctacc gctggtcctg ccgtatggcg atttgtggtt
    11521 cctgcggcat gatggttaac aacgtgccaa aactggcatg taaaaccttc ctgcgtgatt
    11581 acaccgacgg tatgaaggtt gaagcgttag ctaacttccc gattgaacgc gatctggtgg
    11641 tcgatatgac ccacttcatc gaaagtctgg aagcgatcaa accgtacatc atcggcaact
    11701 cccgcaccgc ggatcagggt actaacatcc agaccccggc gcagatggcg aagtatcacc
    11761 agttctccgg ttgcatcaac tgtggtctgt gctacgccgc gtgcccgcag tttggcctga
    11821 acccagagtt catcggtccg gctgccatta cgctggcgca tcgttataac gaagatagcc
    11881 gcgaccacgg taagaaggag cgtatggcgc agttgaacag ccagaacggc gtatggagct
    11941 gtactttcgt gggctactgc tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca
    12001 ttcagcaggg caaagtagaa agttcgaaag actttcttat cgcgaccctg aaaccacgct
    12061 aaggagtgca acatgacgac taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg
    12121 tggaaaaaat tgccgtttta tcgcttttac atgctgcgcg aaggcacggc ggttccggct
    12181 gtgtggttca gcattgaact gattttcggg ctgtttgccc tgaaaaatgg cccggaagcc
    12241 tgggcgggat tcgtcgactt tttacaaaac ccggttattg tgatcattaa cctgatcact
    12301 ctggcggcag ctctgctgca caccaaaacc tggtttgaac tggcaccgaa agcggccaat
    12361 atcattgtaa aagacgaaaa aatgggacca gagccaatta tcaaaagtct ctgggcggta
    12421 actgtagttg ccaccatcgt aatcctgttt gttgccctgt actggtaagg agcctgagat
    12481 gattaatcca aatccaaagc gttctgacga accggtattc tggggcctct tcggggccgg
    12541 tggtatgtgg agcgcaatta ttgcgccggt gatgatcctg ctggttggta ttctgctgcc
    12601 gctggggctg tttccgggtg atgcactgag ctacgagcgc gttctggcgt tcgcgcagag
    12661 cttcattggt cgcgtattcc tgttcctgat gatcgttctg ccgctgtggt gtggtttaca
    12721 ccgtatgcac cacgcgatgc acgatctgaa aatccacgta cctgcgggca aatgggtttt
    12781 ctacggtctg gctgctatcc tgacagttgt cacgctgatt ggtgtcgtta caatctaacg
    12841 catcgccaat gtaaatccgg cccgcctatg gcgggccgtt ttgtatggaa accagaccct
    12901 atgttcaaaa cgacgctctg cgccttatta attaccgcct cttgctccac atttgctgcc
    12961 cctcaacaaa tcaacgatat tgtgcatcgc acaattaccc cgcttataga gcaacaaaag
    13021 atcccgggta tggcggtggc ggtaatttat cagggtaaac cttattactt tacctggggc
    13081 tatgcggaca tcgccaaaaa gcagcccgtc acacagcaaa cgttgtttga gttaggttcg
    13141 gtcagcaaaa catttacggg cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag
    13201 ttaagcgatc ccgcaacaaa atactggcct gaacttaccg ctaaacagtg gaacgggatc
    13261 acgctattac atctcgcaac ctacaccgct ggcggcctgc cattgcaggt gccggatgag
    13321 gtgaaatcct caagcgactt gctgcgcttc tatcaaaact ggcagcctgc atgggcgcca
    13381 ggaacacaac gtctgtatgc caactccagt atcggtttgt tcggcgcact ggctgtgaag
    13441 ccgtctggtt tgagttttga gcaggcgatg caaactcgtg tcttccagcc actcaaactc
    13501 aaccatacgt ggattaatgt accgcccgca gaagaaaaga attacgcctg gggatatcgc
    13561 gaaggtaagg cagtgcatgt ttcgccaggg gcgttagatg ctgaaactta tggtgtgaag
    13621 tcgaccattg aagatatggc ctgctgggta cgaagcaata tgaatccccg tgatatcaac
    13681 gacaaaacac ttcagcaagg gatacaactg gcacaatctc gctactggca aaccggcgat
    13741 atgtatcagg gcctgggctg ggaaatgctg gactggccgg taaatcctga cagcatcatt
    13801 aacggcagtg acaataaaat tgcactggca gcacgccccg taaaagcgat tacgcctcca
    13861 actcctgcag tacgcgcatc atgggtacat aaaacggggg cgaccggcgg atttggtagc
    13921 tatgtcgcgt ttattccaga aaaagagctg ggtatcgtga tgctggctaa caaaaactat
    13981 cccaatccag cgagagtcgc cgccgcctgg cagattctca acgctctgca gtaaaatttc
    14041 atcgggtccg aattttcgga ccttttctcc gcttttcctt gctgtcatct acacttagaa
    14101 aaaaaccagt aaggaaacat ttatgcgcct gctccctctc gttgccgcag cgacagctgc
    14161 atttctggtc gttgcctgca gttctcctac gccgccgcgt ggcgtgaccg tagtgaataa
    14221 tttcgacgcc aaacgttatc tcggtacctg gtatgagatt gcccgttttg atcaccgctt
    14281 tgagcgtgga ctggaaaaag tcaccgcaac atacagcctg cgtgatgacg gcggcctgaa
    14341 tgtcattaat aaaggctata accctgacag aggaatgtgg cagcagagtg aagggaaagc
    14401 gtactttacc ggcgcaccaa ctcgcgctgc gctgaaagtg tcattctttg gtcctttcta
    14461 tggcggttat aacgttattg cactcgatcg ggaataccgc catgcgctgg tttgcggccc
    14521 ggaccgcgac tacctgtgga tactctcccg cacgccaacc atttctgacg aagtgaaaca
    14581 ggagatgctg gcagtcgcga cccgggaagg gtttgatgtc agtaaattta tttgggtaca
    14641 gcagcccggc agttagtgag tgctgagttt cagaccaata atccccaata cgattaacgc
    14701 cagactcgcc aggcgcatcg ggttagcgga ctcaccgagc agcacaatgc cggtgatggc
    14761 cgcgccgact gcgccaatac ccgtccacac ggcataagcc gtccctactg gtaacgattt
    14821 catcgcccag gcaagtagcg ccatactgac aatcatcgcc gtcacggtaa taacactcgg
    14881 cgtcaaacga ctaaagccgt gggtatattt caggccaacg gcccatacca cttccagcag
    14941 accagcaata actaagataa tccaggacat atcaggctcc ggagaattgg ggccgtcccc
    15001 ggtgaaaaga cgcgtttgca ggtcgtcctg caaagctgat ggttgaacgg atattttgcc
    15061 aggtggcagg ggaatttcaa ctattacctt tgtgactatc gcagagaaaa caaacggcac
    15121 gacacagctg tcgtaccgtt attgcttatt gctgcgcttt cgttgctgca ccagaaatcg
    15181 cgttaccgcc atcagaaatg tcttcaccaa cgccacgcgt ggtgttgcag gcagttaata
    15241 ctgttgaaag cacca
//
LOCUS       DAZFKF010000395         2429 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.guided.272, whole genome shotgun sequence.
ACCESSION   DAZFKF010000395 DAZFKF010000000
VERSION     DAZFKF010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.guided.272"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..345
                     /locus_tag="ACHKA1_005674"
     CDS             <1..345
                     /locus_tag="ACHKA1_005674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease subunit R"
                     /protein_id="HFO5683154.1"
                     /translation="QERDFFSNDSHNLRLYVNNEDGRTKFAYLGIISCIYRSISHKYS
                     WGDSVSNRKIKNDVVWLPVKNKSPDFCYINDLISVVHKLVIKDVVQYAERKMNAYRQA
                     ITSRGYDDSGVS"
     gene            870..1817
                     /gene="stxA1a"
                     /locus_tag="ACHKA1_005675"
     CDS             870..1817
                     /gene="stxA1a"
                     /locus_tag="ACHKA1_005675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO5683155.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1827..2096
                     /gene="stxB1a"
                     /locus_tag="ACHKA1_005676"
     CDS             1827..2096
                     /gene="stxB1a"
                     /locus_tag="ACHKA1_005676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO5683156.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          689 a          421 c          568 g          751 t
ORIGIN      
        1 caggagcgag actttttttc aaacgacagt cataatttgc gactctatgt taacaatgag
       61 gatggaagga caaagtttgc ttatttgggc attatttcat gtatttatag atctatttca
      121 cataaatatt catgggggga tagtgttagt aatagaaaaa ttaagaacga tgttgtttgg
      181 ttgccagtaa aaaataagtc acctgacttt tgttatataa atgaccttat ttcagttgtt
      241 cataagcttg tcattaaaga tgttgtccag tacgcagaac gcaaaatgaa cgcatacagg
      301 caagctataa caagtcgcgg ttatgatgat tctggtgtca gttaaaaaac attttacaat
      361 cgtaaaaatc ctcatatcat gataagaatg gttacattgc cacgctgctt aacccgccga
      421 tgcgcgggtt ttttgtatcc ggaatcctgt tagttatacg gaaagtacac agaaaggaag
      481 gtgcgaccac aattaataac aaaatcttaa aaatcgcaca tggcactatt agttttctaa
      541 atattgtgta ttttttgtat tgcaggatga ccctgtaacg aagtttgcgt aacagcattt
      601 tgctctacga gtttgccagc ccccccagtg gctggctttt ttatgtccgt aacatcctgt
      661 gtatcaataa atgttgttgt ctacgtacgt caagtagtcg catgagatct gaccagatat
      721 gttaaggttg cagctctctt tgaatataat tatcattttc attacgttat tgttacgttt
      781 atccggtgcg ccgtaaaacg ccgtccttca gggggtggag gatgtcaaga atatagttat
      841 cgtatggtgc tcaaggagta ttgtgtaata tgaaaataat tatttttaga gtgctaactt
      901 ttttctttgt tatcttttca gttaatgtgg ttgcgaagga atttacctta gacttctcga
      961 ctgcaaagac gtatgtagat tcgctgaatg tcattcgctc tgcaataggt actccattac
     1021 agactatttc atcaggaggt acgtctttac tgatgattga tagtggcaca ggggataatt
     1081 tgtttgcagt tgatgtcaga gggatagatc cagaggaagg gcggtttaat aatctacggc
     1141 ttattgttga acgaaataat ttatatgtga caggatttgt taacaggaca aataatgttt
     1201 tttatcgctt tgctgatttt tcacatgtta cctttccagg tacaacagcg gttacattgt
     1261 ctggtgacag tagctatacc acgttacagc gtgttgcagg gatcagtcgt acggggatgc
     1321 agataaatcg ccattcgttg actacttctt atctggattt aatgtcgcat agtggaacct
     1381 cactgacgca gtctgtggca agagcgatgt tacggtttgt tactgtgaca gctgaagctt
     1441 tacgttttcg gcaaatacag aggggatttc gtacaacact ggatgatctc agtgggcgtt
     1501 cttatgtaat gactgctgaa gatgttgatc ttacattgaa ctggggaagg ttgagtagtg
     1561 tcctgcctga ctatcatgga caagactctg ttcgtgtagg aagaatttct tttggaagca
     1621 ttaatgcaat tctgggaagc gtggcattaa tactgaattg tcatcatcat gcatcgcgag
     1681 ttgccagaat ggcatctgat gagtttcctt ctatgtgtcc ggcagatgga agagtccgtg
     1741 ggattacgca caataaaata ttgtgggatt catccactct gggggcaatt ctgatgcgca
     1801 gaactattag cagttgaggg ggtaaaatga aaaaaacatt attaatagct gcatcgcttt
     1861 catttttttc agcaagtgcg ctggcgacgc ctgattgtgt aactggaaag gtggagtata
     1921 caaaatataa tgatgacgat acctttacag ttaaagtggg tgataaagaa ttatttacca
     1981 acagatggaa tcttcagtct cttcttctca gtgcgcaaat tacggggatg actgtaacca
     2041 ttaaaactaa tgcctgtcat aatggagggg gattcagcga agttattttt cgttgactca
     2101 gaatagctca gtgaaaatag caggcggaga ttcataaatg ttaaatacat ctcaattcag
     2161 tcagttgttg ccggtctgat aatagatgtg ttagaaaatt tctgcatggt gaatccccct
     2221 gtgcggaggg gcgactggtg aacggtatga tctctttgat gatcgtaagc gagaatacgc
     2281 gggtttggtg gcaccagacc gaactcaccg ggaggcaccc ggcaccatgc agtattcaga
     2341 gattaggcat atatccaggc tcctcatcgc aggagccttt ttacatgcaa aaaaaaaacg
     2401 ctcctgggaa gagcggctgg caagaagaa
//
LOCUS       DAZFKF010000396         1584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1808T20880
            SAMN43251631-rid23065733.guided.303, whole genome shotgun sequence.
ACCESSION   DAZFKF010000396 DAZFKF010000000
VERSION     DAZFKF010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251631
            Sequence Read Archive: SRR30293434
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350257.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350257.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:39:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,438
            CDSs (with protein)               :: 5,438
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 60 of 154
            Pseudo Genes (incomplete)         :: 103 of 154
            Pseudo Genes (internal stop)      :: 27 of 154
            Pseudo Genes (multiple problems)  :: 30 of 154
            Pseudo Genes (short protein)      :: 1 of 154
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251631-rid23065733.guided.303"
                     /serotype="O 27:H30"
                     /isolate="1808T20880"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            130..1089
                     /gene="stxA2b"
                     /locus_tag="ACHKA1_005677"
     CDS             130..1089
                     /gene="stxA2b"
                     /locus_tag="ACHKA1_005677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO5683157.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1102..1365
                     /gene="stxB2b"
                     /locus_tag="ACHKA1_005678"
     CDS             1102..1365
                     /gene="stxB2b"
                     /locus_tag="ACHKA1_005678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO5683158.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          427 a          322 c          380 g          455 t
ORIGIN      
        1 gcaatcggtc actggttcga atccagtaca acgcgccata tttatttacc aggctcgctt
       61 ttgcgggcct tttttatatc tgcgccgggt ctggtgccgt attacttcag ccaaaaggaa
      121 tacctgtata tgaagtgtat attgttaaaa tgggtactgt gcctgttact gggcttttct
      181 tcggtatcct attcccggga atttacgata gacttttcga ctcaacaaag ttatgtctct
      241 tcgttaaata gtatacggac agaaatatcg actcctcttg agcatatatc tcaggggacc
      301 acatcggtgt ctgttattaa ccacacccca ccgggcagtt attttgctgt ggatatacga
      361 gggcttgatg tctatcaggc gcgttttgac catcttcgtc tgattattga gcaaaataat
      421 ttatatgtgg ccggatttgt taatacggca acaaatactt tctacagatt ttcagatttt
      481 gcacatatat cagtgcccgg tgtgacaact gtttccatga caacggacag cagttatacc
      541 actctgcaac gtgtcgcagc gctggaacgt tccggaatgc aaatcagtcg tcactcactg
      601 gtttcatcat atctggcgtt aatggagttt agtggaaatg ccatgaccag agatgcatcc
      661 agagcagttc tgcgttttgt cactgtcaca gcagaagcct tacggttcag gcaaatacag
      721 agagaatttc gtctggcact gtctgaaact gctcctgttt atacgatgac accggaagaa
      781 gtggacctca cactgaactg ggggagaatc agcaatgtgc ttccggagtt tcggggagag
      841 ggtggtgtca gagtggggcg aatatccttt aataatatat cagcgatact gggcacagtg
      901 gcggttatac tgaattgcca tcatcagggg gcgcgttccg ttcgcgccgt gaatgaagag
      961 atacaaccag aatgtcagat aactggcgac aggccagtta taaggataaa caatacttta
     1021 tgggaaagta ataccgcagc tgcttttctg aatcgcaggg ctcactcttt aaatacatcc
     1081 ggagaataac aggagttaaa tatgaagaag atatttgtag cggctttatt tgcttttgtt
     1141 tctgttaatg caatggcagc tgattgtgca aaaggtaaaa ttgagttctc taagtataat
     1201 gagaatgata cattcacagt aaaagtggcc gggaaagagt actggactaa ccgctggaat
     1261 ctgcaaccgc tactgcaaag cgcacagtta acaggaatga cggtaacaat caaatcaaat
     1321 acctgtgcgt caggttcagg atttgctgaa gtgcagttta attaatatca gaagcattgc
     1381 tggtttcgtg gtgtgcagca atgtagttac agtgtaatca atgtcacaat tcagtcagtt
     1441 gaaggttgtc tgcccgactg agaatttgtt aaaaaaaatc ctgcatggtg aatccccctg
     1501 agcggcgggg catatcagcg tcacaggtgt ttctgtttta cctctatcct ttctgtgcgg
     1561 gttcaggtgc tgatactgaa ctca
//
